LOCUS       DBNHQR010000001         8343 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_380213, whole genome shotgun sequence.
ACCESSION   DBNHQR010000001 DBNHQR010000000
VERSION     DBNHQR010000001.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8343)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8343)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8343
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_380213"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /gene="yghU"
                     /locus_tag="AC16VR_00005"
     CDS             <1..830
                     /gene="yghU"
                     /locus_tag="AC16VR_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020800808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HIR6009557.1"
                     /translation="VPPKVWKWDTESGGRFANINRPTAGATHDKELPVGEHPLQLYSL
                     ATPNGVKVTVMLEELLDLGKTDAEYDAWLINIGDGEQFGSGFVAINPNSKIPALLDRS
                     TETPTRVFESAAILLYLAEKFGAFLPEDLSSRGECFSWLFWQMGSAPYLGGGFGHFYA
                     YAPEKFEYPINRYTMEVKRQLDVLDQNLAQRRYLIGDEYTIADMATYPWYGAVVLHNI
                     YDAAEFLDVTSYEHVIRWAKEIEERPAVQRGRRVNKVWGSEETQMPERHSASDFGSV"
     gene            859..2205
                     /gene="gdhA"
                     /locus_tag="AC16VR_00010"
     CDS             859..2205
                     /gene="gdhA"
                     /locus_tag="AC16VR_00010"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014755289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HIR6009558.1"
                     /translation="MTITAPDLDQFMRGLRRRNPGESEFHQAVQEVAIDILPYIADKP
                     AYTAKRIFERMTEPDRIVSFRVVWEDDAGNVRVNRGYRVQCNNAIGPYKGGIRFDPSV
                     NQSILKFLAFEQTFKNSLTGLPLGGAKGGADFDVKGKSDSEVMRFCQAFMTELYRHIG
                     PFTDVPAGDIGVGSREDGYLFGQYKRLANRFEGVLTGKALEFGGSHIRTEATGYGVVY
                     MMENMLAHRGESLEGRTCAVSGSGNVAIYTVEKLNELGARVVTMSDSSGFIHDPDGID
                     ADKLAWILDLKTVRRGRVSEYADEFGVEFQEGKRPWAVPCELAFPCATQNEIGKEDAE
                     TLVANGCCAVVEGANMPTVPEALEVFTSARTLLAPSKAANAGGVGVSGLEMSQNSVRL
                     GWTREEVDTRLRQIIKEIHGHCVEHGDEGDYVNYLKGANIAGFRKVAAAMLAYGVM"
     gene            complement(2214..4268)
                     /locus_tag="AC16VR_00015"
     CDS             complement(2214..4268)
                     /locus_tag="AC16VR_00015"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6009559.1"
                     /translation="MQRLFFGLCSCFAAASGQAQPSSSEPIEEVVVIAHPLSGEGLAQ
                     AATVLEGRELDRKRASTVGETLGREAGIHSAQFGKAVGRPVIHGLGGARVRVMEDRID
                     AMDVSVTSADHAVTVEPFVADRVEVLKGSSALLYGSGAIGGVVDVHTGRIPHEVPDRP
                     LTGGIETRFDSNNDGNATSGKLDGAVGDFAWHVDGTWKDGDDYEIPGFAESEGLRALK
                     SAATSGSLGEEDEAHDRLPGSAFDSESYAVGLSRVGEWGFLGGSVSRLEADYGLPGGH
                     GHAEDGDAGGGLGDDHGDTPTLALKQTRTDFELGILDPFGSFTSANLRLGINDYEHAE
                     IEPDGAVATEFANEAWELRAELVYELEAWRGALGLQHSDRDFSAIGEEAYVPPVQTTD
                     SGLFWVAERDFGGFDLEAGARIGRLEHEPLGAPSERFTTYGLSLGAVVPLGAGWRLGL
                     LGDLSSRAPVAEELYSDGPHLATRAFEIGDPALDSEKAFNLSATLEFEGERWSLAGTV
                     YYTGFSDFIYQQATGGVEDGLPVYVYRQEDARFVGVDASVEWRAAAWEGGDLRLRGLF
                     DLVDAKLDVSGNDNLPRIPPMRYGVGAQMSFGPVTASLDYLRVEDQDDAAPGELPTQG
                     YDDLQAYLGVELPFAADHRLTMFVSGRNLTDDEQRLHTSFIKDFAPAPGRTIEAGLRY
                     LF"
     gene            complement(4344..4622)
                     /locus_tag="AC16VR_00020"
     CDS             complement(4344..4622)
                     /locus_tag="AC16VR_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009560.1"
                     /translation="MPMTRLRSVLLVLTLLVFSSQSIAHDHLSDDLEMDPSCLVCSYS
                     DSVPLTGPVSLTPLWADPAGAKAWPPALGPTPRDCPKDYLSRGPPFSR"
     gene            4765..5646
                     /locus_tag="AC16VR_00025"
     CDS             4765..5646
                     /locus_tag="AC16VR_00025"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6009561.1"
                     /translation="MQTWQVGDVKVTQIVELVFTGLDAFLPDATPEAVLPIEWLRPHF
                     ITPEGVLRFSIHALVIDTGARKIIVDTCVGNDKPRDVFPDWHMLQTGFLEDLASAGYP
                     RESIDTVLCTHLHLDHVGWNTMLEGGQWVPTFPNARYLLERSEFAYVAQEAEAEDVEP
                     WLRDMNRAVMADSIRPVVDAGLIDLVDADHRLCEEVRLIPTPGHTIGHVSVHIASRGE
                     SALITGDFIHHPCQLAHPEWSVTTDYDPSDSVSTRERVFAELADGPTLVIGTHWPEPT
                     AGKIASWGDTYQLKTDP"
     gene            complement(5702..7150)
                     /locus_tag="AC16VR_00030"
     CDS             complement(5702..7150)
                     /locus_tag="AC16VR_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009562.1"
                     /translation="MQSAQPDGQVLVADARAASEVLHEVLADTAAAFPGALGPMGFPA
                     DPKAFRSTYPEVMPRFEAARLASGDRLAMARHAVGALQRRLVWQDAGGSRPLADALAE
                     PAAPLELLQTDFPGEPGWRPSVVYRGERWEADRLADFGAELLGRGVVTAQASQALAWL
                     ADNVLDAGVLRLTGRKIAVLGGGAEMAPTRLWLEAGADVLWLDVAPPPPSWQDASAMS
                     GRLYWPAGNADLLAQPREILATLIEFAAGRPLDLGLYAYAPGRARELRLTGVMNELVN
                     AMPPELVGSITVLVSPTTPTGLAEPDLAAMRARREARPGWEAALDNLGLLGRGGGCAT
                     MGEVAATRTVVGIQGASYQAAQYLGKVLVAECWAEEGPLGSAHPAPFRVSANTAAITR
                     TRSLGHPVFAAAFGGAAAFGVETLTPRQSRRINGLLALCDWLRPDRPVPGTVRVHGGI
                     HTLPYPLESALRIAAAIGFARSPRLLRGLFGK"
     gene            complement(7160..7981)
                     /locus_tag="AC16VR_00035"
     CDS             complement(7160..7981)
                     /locus_tag="AC16VR_00035"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6009563.1"
                     /translation="MDLGLEGKKVIITGGSRGLGRAIGETFLREGAVVSFCSRTGRIE
                     PEDEDPRLGGEVRTDGVEQAAEKLAALGEVYGSVVDAADREQLARWVNEAAETMGGID
                     VVVSNTSALGGQPRSPEGWQRQFDVDIMSAVTLWDNAYPFLKASGAGAFVQVSTITAV
                     DYHAYGQACQSYGAFKGALVNYVCQLAYEYMSEGIRANCVSPGPIFVTGGSWDKIQRA
                     LPEYYEENVARQPAGRLGRPEEVADVVAFLASPRASWVTGENIVVDGAFTKYIKY"
BASE COUNT         1546 a         2849 c         2641 g         1307 t
ORIGIN      
        1 acgtcccgcc gaaagtctgg aagtgggaca ccgagagcgg tggacgcttc gccaacatca
       61 accggccaac tgctggcgcg acgcacgaca aggagctgcc ggtcggggag catccgctgc
      121 agctctactc cctggcgaca ccgaacggcg tgaaggtcac cgtgatgctg gaggaactcc
      181 tcgatctcgg caaaaccgac gcggagtacg atgcctggct gatcaacatc ggcgacgggg
      241 aacagttcgg aagcgggttc gtggcgatca accccaattc caagatcccc gccctgctcg
      301 atcgcagcac cgagacgccc acacgggtat tcgagtctgc cgccatcctg ctctacctgg
      361 cagaaaagtt cggcgctttc ctgcccgagg acctctcctc ccggggcgag tgcttctcct
      421 ggctgttctg gcagatgggc agcgcgccct acctgggcgg aggtttcggc cacttctacg
      481 cctacgcgcc ggagaaattc gagtacccga tcaaccgcta caccatggag gtcaagcgcc
      541 agctggacgt gctcgatcag aacctggcgc agcgccgcta tctgatcggc gacgagtaca
      601 ccatcgccga catggccacc tatccctggt acggcgccgt ggtgctgcac aacatctacg
      661 acgccgccga gtttctcgac gtcacgtcct acgagcacgt gattcgctgg gccaaggaga
      721 tcgaggagcg ccccgccgtt cagcgcggcc gccgggtcaa caaggtctgg gggtcggagg
      781 agacccagat gccagagcgt cacagcgcca gcgatttcgg gtcggtttga tgggcttgtc
      841 gaggtcagaa gaagcgccat gacaatcacc gctcccgatc tggaccagtt catgaggggc
      901 ctgcgccgcc gcaacccggg cgagtccgag tttcaccagg cagtgcagga agtggccata
      961 gacatcctgc cgtatattgc tgacaagccc gcctacacag caaagcggat cttcgagcgc
     1021 atgacggaac cggatcgcat cgtctccttc agagtcgtct gggaggacga cgccgggaac
     1081 gtgagggtga accgcggcta tcgggttcag tgcaacaacg ccattggacc ttacaagggc
     1141 ggcatccgct tcgacccgtc ggtcaaccag agcatcctca agtttctcgc cttcgagcaa
     1201 accttcaaga acagcctcac cggtctgccc ctggggggtg ccaagggcgg cgcggacttc
     1261 gacgtaaagg gaaagtccga ctcggaagtc atgcggttct gtcaggcgtt catgacggag
     1321 ctgtaccgac acatcgggcc ttttaccgac gtgcctgccg gagacatcgg ggtcgggtcc
     1381 cgggaggacg gctacctgtt cggtcagtac aagcggctgg ccaaccgctt cgagggcgtg
     1441 ctgaccggca aggcgctcga gttcggtggg agccacatcc gcaccgaagc caccggctac
     1501 ggcgtggtct acatgatgga gaacatgctc gcccatcgag gggagagcct cgagggcaga
     1561 acgtgtgccg tatccggctc cggcaacgtg gccatctaca ccgtggagaa gctaaacgag
     1621 ctcggcgcca gggtggtcac catgtccgac tcatcaggat tcattcacga cccggacggg
     1681 atcgatgcgg acaagctcgc ctggattctg gatctcaaaa ccgtccgccg cggtcgcgtg
     1741 agcgagtacg cggacgagtt cggcgtcgag ttccaagaag gcaaacggcc ctgggccgtg
     1801 ccctgcgagc tcgcatttcc ctgcgcgacc cagaacgaga tcggcaaaga ggatgccgag
     1861 accctcgttg ccaacggctg ctgcgcggtg gtcgagggcg ccaacatgcc taccgtgccc
     1921 gaagcgctgg aggtcttcac cagcgcccga actctgctcg cgccctccaa ggcggcgaac
     1981 gcgggcggag tcggcgtgtc cgggctggag atgagtcaga acagcgtgcg tctgggctgg
     2041 acgcgcgagg aagtcgacac gcgcctgcgc cagatcatca aagaaattca cggtcactgc
     2101 gtggagcatg gcgacgaagg cgactacgtg aactacctca agggcgcgaa cattgccggc
     2161 ttccggaagg tggcggcggc catgctggct tatggggtga tgtgagaagc ccgtcagaac
     2221 aggtaacgca gcccggcttc gatggtacgg ccgggcgccg gggcgaagtc tttgatgaac
     2281 gaggtgtgca gacgctgctc gtcatcggtc aggtttctgc cggagacgaa catcgtcagg
     2341 cgatgatcgg ccgcgaaagg caactccaca cctaggtatg cctgaaggtc gtcgtacccc
     2401 tgggttggca gctcccccgg cgccgcgtcg tcctgatcct cgaccctgag ataatccagc
     2461 gaggccgtga caggcccgaa cgacatctgg gcgccgacgc cgtaacgcat cgggggtatg
     2521 cggggcaggt tgtcgttgcc gctgacgtcg agcttggcat cgacgaggtc gaacagcccc
     2581 cgaaggcgga gatcgcctcc ttcccaggca gcggcccgcc actcaaccga ggcgtccacg
     2641 cccacgaagc gcgcatcctc ctgccggtag acgtagaccg gcaggccgtc ctccacgcct
     2701 cccgtcgcct gctgatagat gaagtccgaa aaaccggtgt agtaaacggt tccggccaac
     2761 gaccagcgct cgccttcgaa ttccagggtc gccgacaggt tgaaagcctt ctcggagtcc
     2821 agtgccgggt ccccgatctc gaaagcgcgg gttgcaaggt gaggcccatc ggaatagagc
     2881 tcctcggcaa cgggcgcccg ggaagagagg tcacccagaa gaccgaggcg ccatcccgcc
     2941 ccgaggggca ccacggcacc aagggagagc ccgtaagtag tgaaccgctc gctcggcgcg
     3001 cccagcggct catgctccag ccggccgatg cgcgcgcccg cttccaggtc gaaacctcca
     3061 aagtcccgct cagcaaccca gaacaggccg ctgtcggtcg tctgcaccgg aggcacgtag
     3121 gcctcttccc cgatggcgga gaaatcacgg tctgagtgct gcagcccgag cgccccgcgc
     3181 caggcctcga gctcgtatac cagctcggcc cgaagctccc aggcctcgtt ggcgaactcc
     3241 gttgccaccg cgccgtccgg ttcgatctct gcgtgctcgt agtcgttgat tcccagccgc
     3301 aaattggcgc tggtaaacga cccgaacgga tccaggatgc caagctcgaa gtcggtacgc
     3361 gtctgcttca gggccagggt gggcgtgtcc ccgtgatcgt ccccgagacc cccgcccgca
     3421 tcgccatctt cggcgtggcc gtgcccccca ggcaggccgt aatcggcttc gagccggctc
     3481 acggacccgc cgaggaagcc ccactcaccc acccgggaaa gaccaaccgc ataggattcc
     3541 gaatcgaacg cgctgcccgg cagcctgtca tgggcctcgt cctcttcccc cagcgacccg
     3601 ctcgtcgccg ccgatttcag cgcacgcaga ccttcggact ccgcaaagcc cgggatctcg
     3661 tagtcgtcgc cgtccttcca ggtaccatcg acgtgccagg cgaaatcacc cacggcgccg
     3721 tcgagcttgc cgctggtggc gttgccgtcg ttgttgctgt cgaagcgggt ttcgatcccg
     3781 ccggtcaacg gccggtccgg cacctcgtgg ggaatgcggc ccgtgtgaac gtccaccacg
     3841 ccgccaatgg cgccagagcc gtagagcagc gcgctggatc ccttcagaac ctccacgcgg
     3901 tcagcaacga acggttccac cgtgaccgcg tgatcggcgc tggtcaccga cacgtccatg
     3961 gcgtctatgc ggtcctccat cacgcgaacc cgcgcgccgc ccagcccgtg aatgaccgga
     4021 cgacccacgg ctttgccaaa ctgagcggag tgaatgcctg cctcccggcc gagcgtctcg
     4081 ccgacggtgg aagcgcgctt gcggtcgagc tcgcgccctt ccagcacggt tgcagcctga
     4141 gcgaggccct caccggagag agggtgagcg atcacgacga cctcttcgat cggctccgat
     4201 gatgatggct gtgcctgccc cgacgcggcg gcgaagcaac tacacagccc gaaaaacaat
     4261 ctctgcatgt cctgttccca tcgattggcg ctcgcccgac gaaagcaggc tgcggcatca
     4321 actcatctga atgcgtgctg gaatcagcgg gaaaagggcg ggccgcggct caggtagtcc
     4381 ttggggcaat cgcggggcgt cgggcccaaa gccggaggcc acgccttggc ccccgccggg
     4441 tctgcccaaa gcggtgtcag ggagaccgga cccgtcaggg gcaccgagtc tgagtagctg
     4501 cagacgaggc acgagggatc catctcgaga tcatcggaca aatgatcgtg cgcgatgctc
     4561 tggctcgaga agacgagcag cgtcagcacc agcagaaccg atcgtaggcg cgtcatcggc
     4621 aagaaagccg ttcctgacat caacgggagt gcggggcgga aactatcagc gatgcaggca
     4681 cggcgcaagc cgagcaccgg ccaaccagcc atgttctaat ctctccgtct gaagcgcgat
     4741 ggccacggat caaggaaacg cccagtgcag acgtggcagg tcggagacgt caaggtcacc
     4801 cagatcgttg agctggtgtt caccgggttg gacgctttcc tgcccgatgc gacgccggaa
     4861 gccgtcctgc ccatcgagtg gctgcggccg catttcatta cccctgaggg cgttctgaga
     4921 ttcagcattc acgcgctggt catcgatacc ggagcaagga agatcatcgt cgacacctgt
     4981 gtcggcaacg acaaaccgag ggacgtgttc cccgactggc acatgctgca gaccggcttc
     5041 ctggaagatc tggcctcggc aggctacccg cgagagtcga tcgatacggt gctctgcacg
     5101 catcttcacc tggatcacgt gggttggaac accatgctcg aagggggcca gtgggtgccg
     5161 accttcccca acgcccgcta cctgctcgaa cgcagcgagt tcgcctacgt ggcccaggaa
     5221 gcagaagcgg aagacgtcga gccctggctt cgcgacatga accgagccgt gatggcggac
     5281 tccatccgcc cggtggtgga cgccggcctg atagatctgg tggatgccga ccatcgcctg
     5341 tgcgaagagg tgcggttgat tccgacgccc ggccatacca tcggccacgt cagcgtgcac
     5401 atcgcctccc gcggcgagtc cgccctgatc accggcgatt tcatccacca cccctgtcag
     5461 ctcgcccatc cggaatggtc cgtgacgacc gactacgacc cttcagattc cgtgagcacg
     5521 agagagcggg tctttgccga gctggccgac ggccctaccc tggtgatcgg cacccactgg
     5581 ccggaaccca ccgccgggaa aatcgccagc tggggcgaca cctaccagct caagacggat
     5641 ccctgagctg ccgctccccg accccaacgg cccgagaccg gaacatcgag ccccgagccg
     5701 atcacttccc gaacaggcct cgcagcagcc gaggcgagcg cgcaaagccg atggcggccg
     5761 cgatgcgcag cgccgactcc aacggatacg gcagcgtgtg gatgccgccg tgcacccgaa
     5821 cggtgccggg cacgggccgg tccgggcgca gccagtcgca aagggccaga agcccgttga
     5881 tgcgccgcga ctggcgggga gtcagcgtct ccacgccgaa tgccgccgcc ccgccaaacg
     5941 ccgcggcaaa gaccgggtga cccagggagc gcgtgcgcgt gatcgccgcg gtgttggcgg
     6001 acacccgaaa cggtgcgggg tgagcgctgc ccagcgggcc ttcctcggcc cagcattccg
     6061 ccacgagcac cttgcccagg tactgggccg cctgatagct ggctccctga atgcccacca
     6121 ccgtgcgggt tgccgcaact tctcccatgg tggcgcagcc tccccctctt cccagcaggc
     6181 cgagattgtc gagcgcggct tcccagccgg ggcgggcttc cctgcgcgcg cgcatggcgg
     6241 ctaggtctgg ctcggcgagg ccggtcggcg tggtcggcga gaccagaacg gtgatgctgc
     6301 ccaccagctc cggcggcatc gcgttcacca gctcgttcat gacccccgtg agtcgcagtt
     6361 cccgcgcccg ccccggggca taggcgtaca ggccgaggtc gaggggcctg ccggcggcaa
     6421 actcgatcag cgtagcgagg atttcacgag gttgagcgag cagatcggcg tttccggccg
     6481 gccagtacag tcgaccggac atggccgacg cgtcctgcca gctcggcggc gggggcgcca
     6541 cgtcgagcca gagcacgtcg gcccctgcct cgagccaaag ccgcgtgggc gccatctcag
     6601 cgccgccgcc gagaaccgcg atcttgcgtc ctgtcagcct cagcactccg gcatccagca
     6661 cgttgtcagc cagccaggcg agggcttggg acgcctgcgc ggtcacgacg ccgcggccaa
     6721 gcagctccgc gccgaaatcg gcgagccgat cggcttccca acgctctccc cgatacacca
     6781 cgctcggccg ccagccgggc tcccccggaa agtcggtctg caggagctcg agcggcgcag
     6841 ccggctccgc cagcgcgtcg gccaggggcc ggctaccgcc ggcatcctgc cagaccagcc
     6901 gccgttgcag ggcgccgacc gcgtggcgtg ccatggcaag gcgatcgccg ctggccagac
     6961 gggccgcctc gaaacggggc atcacctcgg gatacgtgga acggaatgcc ttgggatccg
     7021 cgggaaaccc catcggaccc agggcgccgg gaaaggccgc ggcggtgtcg gcaagcacct
     7081 cgtggagcac ctcggacgca gcgcgcgcgt ctgcgaccag gacctgccca tcgggttgtg
     7141 cggactgcac tgcgaacggc taatacttga tgtacttggt gaaggcaccg tccacgacga
     7201 tgttctcccc cgtgacccag gacgccctgg ggctggccag gaaggccacc acgtcggcca
     7261 cctcctccgg cctgcccagc cgacccgcgg gctgacgcgc cacgttctcc tcgtaatact
     7321 cgggcagggc ccgctgaatc ttgtcccagg agccgccggt gacgaagatc ggccccgggg
     7381 atacgcagtt ggcgcggatg ccttcgctca tgtactcgta ggcaagctgg cacacgtaat
     7441 tgaccagcgc tcccttgaaa gcgccgtagc tctgacacgc ctgcccgtaa gcgtggtagt
     7501 ccacggcggt aatcgtcgag acctgcacga acgcgcccgc tcctgaagcc ttgaggaacg
     7561 ggtaggcgtt gtcccacagc gttaccgcgg acatgatgtc cacgtcgaac tgccgctgcc
     7621 atccttccgg cgaacgcggt tgccccccga gcgcgctggt gttcgaaacc accacgtcga
     7681 tgccgcccat ggtctcggcc gcttcgttca cccagcgcgc gagctgctcg cggtcggcgg
     7741 cgtccacgac ggacccgtac acctcgccaa gggcagccaa tttctctgcc gcctgctcga
     7801 cgccgtccgt ccggacctcc ccgccaaggc gcggatcttc atcctcgggt tcgatacgcc
     7861 cggtgcggga acagaacgac acgacggctc cttcgcgcag gaacgtctcc ccaatggcgc
     7921 gacccagacc ccgcgagccg ccggtgatga tgactttctt tccttcgagc ccaagatcca
     7981 tgactcacac ccccaaaacc cgatcaggca agtatagcga acccggccag cgccgctgcc
     8041 gtgcacccga ctctcaccgg ttggagtgtt ccccaaagtc atccggcgaa cacgccgaac
     8101 tcacaggcga tctccggatg cgtctggtcc gggccagtga tactgttggg tgttgggggc
     8161 aagtcgtgcc gaggctcatc acccatcggc cgttgcaccg accatctccg ccacgggagg
     8221 gacaacatgc cacgagttct gtacagcaat gcgaacctgc tcgacggtga gcacgccgcc
     8281 agacccggat cctcggtcat cgtggaagac gggcacatcc ttgccgtcgg caccccggag
     8341 tcc
//
LOCUS       DBNHQR010000002         4547 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_633707, whole genome shotgun sequence.
ACCESSION   DBNHQR010000002 DBNHQR010000000
VERSION     DBNHQR010000002.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4547)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4547)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4547
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_633707"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="AC16VR_00040"
     CDS             complement(<1..470)
                     /locus_tag="AC16VR_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017520572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HIR6009564.1"
                     /translation="MTRSEHTRSDLLGHRSGMTFAGELSFLRRKYTRDIEGADLVVTG
                     LPFDMATTFRPGARLGPQAIRAASVQLAGIAGRAFPYGEDPFATLAVADCGDCFIDSG
                     YPQHGAAAIEAHARDIIARGCKLLSFGGDHFVTYPLLRAHAEKHGPMSLIHFDA"
     gene            complement(472..1704)
                     /locus_tag="AC16VR_00045"
     CDS             complement(472..1704)
                     /locus_tag="AC16VR_00045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6009565.1"
                     /translation="MRTYPGLDGSCEADVCIVGGGYTGLSAALELGRRNYDVVVLEAH
                     RVGSGASGRNGGVLGMGQRKDQLELESWLGADAARKLWRIACDANDLVRDRIAEFGID
                     CDLKSGELTAAHRERYEKTLWSYAEHLERDYGYSDRRIVSREEVAKMLGADTLYGGYV
                     DLRAGHVHPLNLAIGLARAASDHGATLHEQTPALGYRQEGDRVLVRTASGDVRARFLI
                     IACNGYLGRFEKGAERYQMPINNFILATEPLGAEMGRAINRDDLAVVDTRFVVNYFHN
                     SPDHRLIFGGGENYSSRFPKDIKAFVRRRMLQIYPQLESARIDFGWGGTLAITMRRMP
                     QFGRKGANVYWAQGYSGHGIAMANMGGKLVADAIMGQAEEFDTFAGLKHLPFPGGRYL
                     RWPALVSGMLYYAFLDRL"
     gene            complement(1776..3089)
                     /locus_tag="AC16VR_00050"
     CDS             complement(1776..3089)
                     /locus_tag="AC16VR_00050"
                     /inference="COORDINATES: protein motif:HMM:NF012348.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HIR6009566.1"
                     /translation="MGKPSEMPDELELLLSDMNGVPRGKTLRGISYSEAHPPHLAEAI
                     FFQTITGGYAAAMETYDPNDRDLLLRPDWSTYVPTPWKPDTGQVICESLDQAGERLPY
                     DPRNVLRRVLDAYESMGLRPVVAPEVEFYLLEAVSSHDLVLTPATGRSGAAEFGGESF
                     SPDALEKFMPIVAEIQQACRQTGLELSAMVHEMGPAQIELNVAHGDALSRADQLFQLK
                     RLIKGCARKHGALASFMAKPLADLPGNGLHLHCSMEDETGRNIFALNRRRAPAVLQHF
                     IAGLQAHLPQAFALVAPNVNSYKRFVRDLSAPINLEWGYDNRTTGFRVPFSDARAGRV
                     ENRVAGADANPYLIIAATLACGLLGMRAAIKPTKPIADDAYGLKPALPEDLGEALRIL
                     EQSEGLIDLLGEAFVEVFVSVKRGELDHFGKTITQWEVGYLGSTL"
     gene            complement(3124..3876)
                     /locus_tag="AC16VR_00055"
     CDS             complement(3124..3876)
                     /locus_tag="AC16VR_00055"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011536407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HIR6009567.1"
                     /translation="MSRPVVLIPCDVKRYGEYPFHCVGEKYINAVAHGAGAMPLLMPA
                     WGRGAELRELTDLFDLDAILDRVHGVFLPGSTSNVHPHRYGGADLDMLHDEQRDAVVF
                     PLIERALSRGLPLLAVCRGLQELNVALGGTLHPRIHEVPGYLDHRENDELPRDAQYGL
                     VHAVDAVHGGMLADLVGAKRFLVNSLHAQGIAELGAGLRVEARAEDGLIEAVSMVGGW
                     VLGVQWHPEWHFTDDTASAKLFAGFGEALRAA"
     gene            3954..>4547
                     /locus_tag="AC16VR_00060"
     CDS             3954..>4547
                     /locus_tag="AC16VR_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009568.1"
                     /translation="MSNLALISRDVAEILPAAGTDEFAPSVVAMLRDQVNIDEACMIV
                     YPRDESPRIAHRETQKPGDEPNLDTFIKGPFLLDPYYVAAAREGRFGFFPLSHLAPTG
                     FKTSEYYRTYYRFSGIFDECGYLTPIGDGGFANLSLARADGNQPFESSTLRCLADLAP
                     LVTALTQHHWRLLPANPSTEQISLRTQLESALESFGES"
BASE COUNT          841 a         1540 c         1376 g          790 t
ORIGIN      
        1 tgagcatcga aatgaatcag cgacatgggc ccatgctttt cagcgtgcgc ccggagcagc
       61 gggtaggtca cgaaatgatc gcccccgaac gacagaagtt tgcagcctcg agcaatgatg
      121 tcccgggcat gggcttcgat ggccgcagcc ccatgctggg ggtaaccgga atcgatgaag
      181 cagtcaccac agtcggcaac agctagcgtg gcgaacggat cctccccgta aggaaacgcc
      241 cgcccggcaa ttcccgccag ctgcacgctg gccgcgcgga tggcctgagg gcccagtcgc
      301 gcgcccggcc tgaaagtcgt cgccatgtcg aagggcaaac cggtcaccac cagatctgcg
      361 ccctcgatgt ccctggtgta cttgcggcga aggaaggaaa gctcgcccgc aaaggtcatc
      421 ccggaacggt gccccagcag gtcggatctc gtgtgctcgg agcgcgtcat ctcatagacg
      481 atccagaaaa gcgtagtaaa gcatacccga aaccagcgcc ggccagcgca gataccgccc
      541 tcccgggaag ggcaggtgct tgagcccggc aaacgtgtcg aattcctcgg cctggcccat
      601 aatcgcgtcc gcaaccagct taccgcccat gttggccatc gcgatcccgt ggcccgagta
      661 tccctgagcc cagtagacgt tcgctccctt gcgcccgaac tgcggcatgc gccgcatggt
      721 gatcgcaagc gtgccgcccc atccgaagtc gatccgagcg ctttccagct gaggatagat
      781 ctggagcatc cgccgccgca cgaacgcctt gatgtccttc ggaaaccgcg acgagtagtt
      841 ctcgcccccg ccgaagatca gccgatggtc cggcgagttg tggaaatagt tcaccacgaa
      901 gcgggtatcg accacggcga gatcgtcccg attgatcgcg cgccccatct cggcccccag
      961 gggttccgtg gccaggatga agttgttgat gggcatctga tagcgctcgg cgcccttctc
     1021 gaaacgtccc agataaccgt tgcaggcgat gatcaggaat cgggcgcgta cgtcgcctga
     1081 tgcggtgcgc accaagaccc tgtccccctc ctgtcggtag cccagggccg gcgtctgctc
     1141 gtgaagcgtt gccccgtgat cgctggccgc acgggcgagg ccgatggcca ggttcagcgg
     1201 gtgcacgtgg cccgcgcgca aatccacgta gccgccgtac agggtgtccg cgccgagcat
     1261 ctttgccact tcctcgcggc tgacgattct ccggtccgag tatccgtagt cacgctccag
     1321 atgctcggcg tacgaccaca acgttttctc gtaacgctcg cggtgcgctg ccgtgagctc
     1381 gccgcttttc aggtcacagt cgatcccgaa ttcggcgatg cggtcacgca ccaggtcatt
     1441 ggcatcacag gcgatccgcc acagcttccg ggccgcatca gcgcccagcc aggactcgag
     1501 ctccagctgg tcttttcgct ggcccatgcc gagcacgccg ccgttgcgtc cggacgctcc
     1561 cgagccgacc cgatgggctt ccagcaccac cacgtcgtaa tttctgcggc cgagctccag
     1621 ggcggccgac agcccggtgt agccgccgcc gacgatgcag acgtcggctt cgcaggatcc
     1681 atcgagaccg gggtaggtcc gcattgtctt tgcgctgtgg taataccagg actcgtcaaa
     1741 cccgaccctg ggatctcccc cgccccgaga tcggctcaca gcgtgctgcc caggtagccg
     1801 acttcccact gagtgattgt cttgccgaaa tggtcaagct cgccacgctt gaccgacacg
     1861 aagacctcca cgaacgcctc gcccaggaga tcgatgaggc cttcgctttg ttccagaatc
     1921 cgcagcgctt cacccagatc ttccggaagc gccggcttca gaccgtaggc atcgtcggcg
     1981 atgggcttgg tcggcttgat ggcagcacgc atacccagca gtccgcaggc gagcgttgca
     2041 gcaatgatga ggtaagggtt cgcgtccgca cctgccacgc ggttctcgac gcgccctgct
     2101 cgggcatcac tgaagggcac gcggaagccg gtggtccggt tgtcgtaacc ccattccagg
     2161 ttgatgggcg ccgacagatc ccgcacgaag cgcttgtagg agttcacgtt gggggcaacc
     2221 aacgcgaacg cctggggcag atgcgcctgc agtcctgcaa tgaagtgctg gagcacggcg
     2281 ggcgcccggc gacgattgag cgcaaagata ttgcgcccgg tctcgtcctc catgctgcaa
     2341 tgcaggtgca gcccgttccc cggcaggtct gccagcggct tggccatgaa gcttgccagg
     2401 gcgccgtgct tgcgcgcgca gcccttgatc agccgcttga gctgaaacag ctgatccgcg
     2461 cgggagagcg cgtcgccgtg ggccacgttg agctcgatct gggcgggtcc catctcgtgg
     2521 accatcgccg agagctcgag acccgtctgg cgacaggctt gctgaatctc ggccacgatg
     2581 ggcatgaact tctccagcgc atcggggctg aaggactcgc cgccgaactc ggcagccccc
     2641 gaccttccgg tcgccggcgt cagcaccagg tcatggctgg acacagcttc cagaagatag
     2701 aactcgactt cgggagcgac cacggggcga agtcccatgg actcgtaagc atccagcacc
     2761 ctccgcagca cgttacgcgg atcgtagggc aggcgttcac ccgcctggtc gaggctttcg
     2821 cagatcacct ggcccgtgtc cggcttccag ggggtcggga cgtaggtcga ccaatccgga
     2881 cgcaaaagca gatcgcgatc gttggggtcg tatgtctcca tggcggccgc gtacccgcct
     2941 gtgatggtct ggaaaaagat cgcttccgcc aggtggggcg ggtgcgcttc ggaatagctg
     3001 attccacgca gggtcttgcc gcggggcacg ccgttcatgt ccgacagcag cagttccagt
     3061 tcgtccggca tttccgatgg cttgcccatc cgctccctcc tcagtcgacc agccgggcag
     3121 caatcacgct gcccgcaaag cttcgccaaa gccggcgaac agcttggcag acgcggtgtc
     3181 atccgtgaag tgccactccg gatgccactg cacgcccagc acccagccgc cgaccatgga
     3241 cactgcttca atcaagccgt cctcggcccg tgcctccacg cgcagcccgg cccccagttc
     3301 ggcgataccc tgggcatgca gcgaattgac gagaaaacgt ttcgcgccga ccaggtctgc
     3361 aagcatgccg ccgtgaaccg catcgacggc atgaaccagc ccgtactggg catcccgcgg
     3421 cagctcgtcg ttctcccggt gatccagata gccgggcacc tcgtgaatcc gcggatgcag
     3481 ggtgccgccg agagccacgt tcagctcctg caaacccctg cataccgcga gcagcggcag
     3541 accgcgtgac agggcgcgct cgatgagagg aaacacgacg gcgtcgcgct gctcgtcatg
     3601 gagcatatcc aggtccgcgc ccccgtagcg gtgggggtga acgttgctcg tagatcccgg
     3661 caggaatacc ccatggaccc ggtcgaggat cgcgtccaga tcgaacagat ccgtcagctc
     3721 gcgcagctct gcgccccgac cccacgccgg catgagcagg ggcatcgccc ccgcaccgtg
     3781 ggcgaccgca ttgatgtact tctcgcccac gcagtggaac gggtactcac catacctctt
     3841 cacgtcgcag ggaatgagca cgaccggcct gctcatcgcc tccctccggc tgcgtggcga
     3901 gtcggcctgg ccggcgataa gatgtggtga tcggcgacgg atcgtgggca aacatgtcga
     3961 atctggcact catcagtaga gacgtcgctg agatcctgcc ggcagccggg accgatgaat
     4021 tcgccccgag cgtcgtcgcg atgctcaggg atcaggtgaa catcgacgag gcctgcatga
     4081 tcgtctatcc ccgcgacgag tccccgcgca tcgcccaccg cgaaactcag aaaccagggg
     4141 acgaacccaa tctcgacacc ttcatcaagg ggcccttcct gctcgatccg tattacgtcg
     4201 ctgcagcccg ggagggccga ttcggattct ttccgctttc ccatctcgcc cccaccgggt
     4261 tcaagacaag cgagtactac cgaacctact accggttcag cggcatcttc gacgagtgcg
     4321 gctatctgac ccccatcggg gacggcggct ttgcgaatct ttccctggct cgcgcagacg
     4381 gcaaccaacc gttcgagtcc tccacactca gatgcctggc agatctcgcg cctctggtca
     4441 ccgcgctcac tcagcatcat tggcgactgc tacccgccaa cccgtctacc gagcagatca
     4501 gcctgcgaac gcagctggag tcggcgctgg agagctttgg cgaatcg
//
LOCUS       DBNHQR010000003         2114 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_633737, whole genome shotgun sequence.
ACCESSION   DBNHQR010000003 DBNHQR010000000
VERSION     DBNHQR010000003.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_633737"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="AC16VR_00065"
     CDS             <1..342
                     /locus_tag="AC16VR_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008020335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HIR6009569.1"
                     /translation="QNSYRGLVRTTPSAHGARNYTQCDSLLIGGASGAHTFPYIESSN
                     PSAIVEHEATTSKVSDEQLFLCQQRGIDAEKAVSMIVNGFCKEVFRELPMEFAVEAAK
                     LLEVSLEGAVG"
     gene            339..1085
                     /gene="sufC"
                     /locus_tag="AC16VR_00070"
     CDS             339..1085
                     /gene="sufC"
                     /locus_tag="AC16VR_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018402757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HIR6009570.1"
                     /translation="MSFLEVRDLHVTVAEKEILKGLNLRIESGEVHAIMGPNGSGKST
                     LASVLAGRPGFQITGGSISFLDQDIAHLEPDEIAHLGIFLAFQYPVEIPGVSNMEFLK
                     ASVDSLHAARGEQPLSTVEFMKAVRTVATDLDLNPEFLKRGVNEGFSGGEKKRNEILQ
                     LLLLQPRLAVLDETDSGLDIDALQVVAAGVNRFSAPGNAVLLITHYQRLLNYIVPDAV
                     HVLSQGQIVRSGGKELALELEEKGYGWITE"
     gene            1082..>2114
                     /locus_tag="AC16VR_00075"
     CDS             1082..>2114
                     /locus_tag="AC16VR_00075"
                     /inference="COORDINATES: protein motif:HMM:NF013614.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HIR6009571.1"
                     /translation="MSDATAQMRDLAGTAPSWFDAQAGLARLDRGLTAPAIREYWKYT
                     PIQPFVTALVEAAHADGPAPEIHNADQPGITVCPMGELAAHQAVTVAAMAGEIDPDRY
                     PLADLALLNGSVGTFCHVAESLPQPLILKSGSNANQATFIHVAAGAEVELQESAPTGP
                     SNGQLIVVRVEAGGRVRHARCALDRLAAHWSLLVAILADNAAYELNQHHFGGRRRRSD
                     CHFVLAGPGATANVTAAFLADEGSHIDQQFVVEHRSRDTVSRQKVHGIGTGRSRCVFN
                     GRIHIHEGATASDATLTNRNLSLHPQAEIDTKPELEIYTDDVRCAHGATVGQLSEDSL
                     FYLRSRGVPE"
BASE COUNT          412 a          694 c          663 g          345 t
ORIGIN      
        1 cagaacagct accgcgggct ggtgcgcacg acgccatccg cccacggtgc ccgcaactac
       61 acccagtgcg actccctgct gatcggcggc gcgtccggag cgcacacgtt cccctacatc
      121 gaaagcagca acccctcggc gatagtcgag cacgaggcga ccacctccaa ggtgagcgac
      181 gagcagctgt tcctgtgcca gcaacggggc atcgacgccg agaaggcggt cagcatgatc
      241 gtcaacggat tctgcaaaga ggtgttccgg gaactgccca tggagttcgc ggtggaggcg
      301 gcaaaacttc tggaggtcag tctggaaggg gccgtaggat gagctttctc gaagtccgcg
      361 atctccacgt caccgtcgcc gagaaagaaa tcctgaaagg gttgaacctg cgcatagagt
      421 ctggtgaggt tcacgcgatc atgggtccga acgggtccgg caaaagcacg ctggccagcg
      481 tcctggccgg acggccgggt tttcagatca ccggcggcag catctcgttt ctggatcagg
      541 acatcgccca cctggagccg gacgagatcg ctcatcttgg catcttcctc gctttccagt
      601 accccgtaga gatccccggg gtgagcaaca tggagtttct caaggcctcg gtggacagcc
      661 ttcacgcggc ccgcggcgaa caacccctga gcaccgtgga gttcatgaag gcggtccgta
      721 cggtcgccac ggacctcgat ctcaaccccg aattcttgaa gcgtggcgtt aacgagggtt
      781 tctcgggtgg cgagaagaag cgcaacgaga tccttcagct gctgctgctc caaccaaggc
      841 tggcggtcct cgacgagacc gattcggggc tggacatcga cgcgcttcag gtcgtcgccg
      901 caggcgtgaa ccgtttcagc gcgccgggca acgccgtgct gctcatcacg cactaccaga
      961 ggcttctcaa ctacatcgtg cccgacgccg tgcacgttct gtcacagggc cagatcgtga
     1021 ggtcgggcgg gaaggagctg gcgctggaac tcgaagagaa gggatacgga tggatcaccg
     1081 aatgagcgac gcgaccgcgc aaatgaggga tctggccggt accgcaccgt cctggttcga
     1141 cgcgcaagcc gggctcgcgc gcctggatcg agggctgact gcccccgcga taagggaata
     1201 ctggaagtac acgcccatcc agccgttcgt gacggccctc gtcgaggcag cgcatgccga
     1261 tggccccgca cccgagattc acaatgccga tcagccgggc atcacggtct gccctatggg
     1321 cgagctggcc gcgcaccagg ctgtcaccgt tgccgccatg gcgggggaga tcgatcccga
     1381 tcgttatcct ctcgcggatc ttgcccttct caacggcagc gtcgggacct tctgccacgt
     1441 cgcagaaagc ctgccgcagc ctctgattct aaaatcgggt agtaacgcca atcaggccac
     1501 gttcatccac gtggcggcgg gcgccgaggt cgagctgcag gaaagcgcac cgaccgggcc
     1561 gagcaacggc cagctcatcg tcgtgcgggt agaggccggc ggacgggtcc ggcatgcacg
     1621 ttgcgccctg gaccgcctgg cggcgcattg gtcactgctc gtcgcgatcc tggccgataa
     1681 tgcagcctac gagctgaacc agcatcactt cggcggtcgc cgccggcgca gcgattgcca
     1741 tttcgtgctg gccgggcccg gagcgacagc gaacgtaacg gccgctttcc tcgccgacga
     1801 gggcagccac atcgatcagc agttcgtggt cgagcaccgc tcgcgcgaca ccgtgagccg
     1861 acaaaaggtc cacggcatcg gaaccggccg cagccgttgc gtgttcaacg gcaggatcca
     1921 cattcacgag ggcgccaccg caagcgatgc caccctgacc aatcgaaacc tctcactgca
     1981 ccctcaggcc gagatcgaca ccaagcccga gctcgagatc tacaccgacg acgttcgctg
     2041 cgcccacggc gcaacggtcg ggcagctctc cgaggacagc ctcttctacc ttcgctcgcg
     2101 cggcgttccg gagg
//
LOCUS       DBNHQR010000004         2894 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_887179, whole genome shotgun sequence.
ACCESSION   DBNHQR010000004 DBNHQR010000000
VERSION     DBNHQR010000004.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_887179"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="AC16VR_00080"
     CDS             <1..547
                     /locus_tag="AC16VR_00080"
                     /inference="COORDINATES: protein motif:HMM:NF013832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008273 - calcium, potassium:sodium
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006874 - intracellular calcium ion
                     homeostasis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HIR6009572.1"
                     /translation="FMTVDQVVRGRQDPLLVGAGQVGAASERGNGGSRNWAEVLLGVA
                     ALAGGGHLTIQSGADLAEILGWEKVLVGMVIVAIGTSLPELVTSVIAAVRREPDLCVG
                     NLVGSNIFNGLLVLPISALLRPIDVPAHGVTDVLASFFVAAALIPIFIMGRSRLGRPV
                     GSLLILAYAGYIGYRLATGTA"
     gene            566..2296
                     /locus_tag="AC16VR_00085"
     CDS             566..2296
                     /locus_tag="AC16VR_00085"
                     /inference="COORDINATES: protein motif:HMM:NF013283.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0097347 - TAM protein secretion complex
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HIR6009573.1"
                     /translation="MGAFSRSMMRVAAGLLALLVWPAAALGDVSIEGVDEEVRTGILA
                     FIALDELPCDAPRWWVQRGYRDAVEGSLRALETYGYYRATVQADLVWGEECWTASIDV
                     VPGEPVRLGAVVIEVSLPLADEPDMAALLGNPGLASGERFSHASYETAKGRVLDLARD
                     LGYLDAAFNRNRVLVDAEAGLADVELALESGLRYRFGPIEIEQDDLRPKLFDRFLTVS
                     EGEDFDGRELRDTQRRLMESDYYDRVLVAPVLDRREDGRVPISVQADASSRRNILLGA
                     GFATDTGPRVRADARYRRVNRKGHRARGGLLGSKVLSEANVEYGLPYGDPTHQWLILK
                     GSVAYEETETARSFRQTVGVARTHRRGDHWTETNYVEYRFEDFDVGDQEGDSDLLLIG
                     TSWSYTSTVDAPRALSGRTITFDVHGASKALASDTDFLKLVVHGKQIVPLGERFRLIG
                     RISSGWTFKSDFDELPPSERFFAGGDSSVRGYEYESLGPEENDAVVGGTRLLTGSLEL
                     DMLIRPKWSVALFVDSGSAFNDSPDFSTGVGAGLRWYSPVGPIRLDLAHPLDDPDNDF
                     RIHISFGPDL"
     gene            2293..>2894
                     /locus_tag="AC16VR_00090"
     CDS             2293..>2894
                     /locus_tag="AC16VR_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009574.1"
                     /translation="MKRLVAGIVGVLLATVFLVIAAVAVLMLSEPGSRFLVERATRLL
                     PLEVEGVTGSLVGEMRFQRLRYELEGQIVEAQGLEVAFDPMAFLLKDEVRFRYVRAVR
                     LRVETRGGEATSAGLGRFELDAPPITLVIDSLAVQVAEILDLPPSSMTLAGRWGAREL
                     SLAQVAVASEVLELRLSGSLANGREPALDLIGSWSLPGTS"
BASE COUNT          450 a          887 c          999 g          558 t
ORIGIN      
        1 cttcatgacc gtcgatcagg tcgttcgcgg ccgccaggac ccgctgctgg tcggcgccgg
       61 ccaggtcggc gctgcctccg agcggggcaa cggcgggagc cgtaactggg cggaagtgct
      121 gctcggcgtg gccgctctgg ctggtggtgg ccatctcacc attcaaagcg gcgcggacct
      181 ggcggagata ctcggctggg aaaaggtgct tgtgggaatg gtcatcgtgg cgatcgggac
      241 cagcctgcct gagctcgtca cgtcggtgat tgcggccgtg cggcgcgaac cggacctctg
      301 cgtgggcaat ctggttggat cgaacatctt caacgggctg ctggttctgc cgatcagcgc
      361 cttgttgagg ccaatcgacg tgcctgccca cggcgtgacg gatgtgctcg catctttttt
      421 tgttgccgcc gcgctgattc cgatcttcat catggggaga agccgactcg gtagacccgt
      481 tgggtccctg ctgatactgg cctatgcggg ttacatcggt taccgactcg ccaccggaac
      541 ggcttaggtc gcggccgcaa tgtccgtggg tgcattctcc cgcagcatga tgcgcgttgc
      601 cgcgggcctg ctcgcgttgc tcgtatggcc cgcggccgcc cttggggacg tgtccatcga
      661 aggggtcgac gaggaagtcc gaacgggcat tcttgccttc atcgccctgg acgagctgcc
      721 ctgcgatgct ccccgctggt gggttcagcg aggttatcgg gacgccgtgg agggatccct
      781 cagggccctg gaaacctacg ggtactatcg ggccacggtg caggcagatc tcgtctgggg
      841 agaagaatgc tggacggcgt ccatcgacgt cgtgcccggt gagccggtcc gccttggcgc
      901 agtggtcatc gaggtgagcc tgccgctcgc cgacgagccg gacatggcgg cgctgctggg
      961 caatcccggc ctggcctcgg gcgaacgttt cagccacgcc tcctacgaga ccgccaaggg
     1021 acgcgttctg gacctggccc gggacctcgg ctatctcgac gctgccttca accgtaatcg
     1081 ggtgttggtc gatgcggaag cgggcctggc ggacgtcgag cttgccctcg aatcgggcct
     1141 tcgctaccga ttcggaccga tcgagatcga gcaggacgat ctgcgtccga agcttttcga
     1201 tcgctttctg acggtctccg aaggcgagga cttcgacggg cgcgagctgc gggataccca
     1261 acgccgtctc atggaaagcg actactacga tcgggtgctg gtggcccccg tgctggatcg
     1321 gcgcgaggat ggacgggtgc ccatcagcgt gcaggcggat gcctcgtcgc gccgcaacat
     1381 cctcctcggt gcgggctttg ccacggacac agggccgcga gtgcgcgcag atgcacgcta
     1441 tcgccgggtc aatcgcaagg gccatcgcgc tcgagggggc ctgctgggct cgaaggtgct
     1501 cagcgaggcc aacgtggaat acggactgcc ctatggagat ccgactcatc agtggctgat
     1561 cctcaaaggc agcgtcgcct acgaggagac cgagacggcc cgcagcttcc ggcagaccgt
     1621 gggcgtcgcg cgcacccacc ggcggggcga tcactggacc gaaacgaact acgtcgagta
     1681 ccgtttcgag gacttcgacg tcggggatca ggagggcgat tccgatctgc tgctgatagg
     1741 cacgagctgg tcttacacga gcaccgtgga tgcgccacgt gctctttcgg gcaggacgat
     1801 caccttcgac gtgcatggcg caagcaaggc gctcgcctcg gacacggatt tcctgaagct
     1861 cgtcgtgcac ggcaagcaga tcgtgccgct gggcgagcgg tttcgcctga tcgggcggat
     1921 ctcgtcgggg tggacgttca agagcgactt cgacgagctg ccgcccagcg aacggttctt
     1981 tgctggcggc gacagcagcg ttcggggcta cgagtacgaa tccctcggtc ctgaggaaaa
     2041 cgatgccgtc gttgggggca cccggctgct gacgggctcc ctggagctgg atatgctgat
     2101 ccggcccaag tggtcggttg ccttattcgt cgactcgggc tcggcgttca acgacagtcc
     2161 cgatttcagc acgggcgtgg gcgcgggtct gcgctggtac tcgccggttg gcccgatccg
     2221 gctggacctg gcccatcccc tcgacgatcc ggacaacgat ttccgcattc acatcagctt
     2281 cgggccagat ctgtgaaacg tcttgttgca ggtattgtcg gcgttctcct ggcaaccgtt
     2341 ttcctggtga ttgccgccgt cgccgtgctg atgctgagcg agccggggag ccggtttctg
     2401 gtcgagcgcg caacccgcct gcttccgctc gaggtagaag gcgttacggg aagcctggtt
     2461 ggcgagatgc gcttccagcg tctgcgctac gagctcgaag ggcagatcgt cgaggcccag
     2521 ggactcgagg tcgccttcga tccgatggcc ttcctgctca aagacgaagt gcgtttccga
     2581 tacgtgcggg ccgtgcgctt gcgcgttgaa acccgtgggg gagaagcgac atcggcaggc
     2641 cttggccggt tcgagctcga cgcgccgccg attaccctgg tgattgactc gctggccgtg
     2701 caggtggcgg aaattctgga tctgccgccg tcgagcatga cgctggccgg gcgctggggc
     2761 gccagagagc tgagcctcgc tcaggtggcg gtggcctcgg aagtgctgga gctccgactt
     2821 tccggatcac tggcaaacgg ccgcgaaccg gcgctcgacc tcatcggttc gtggtcgctg
     2881 cccggcacat cctg
//
LOCUS       DBNHQR010000005         1979 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1013930, whole genome shotgun sequence.
ACCESSION   DBNHQR010000005 DBNHQR010000000
VERSION     DBNHQR010000005.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1013930"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="AC16VR_00095"
     CDS             complement(<1..329)
                     /locus_tag="AC16VR_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009575.1"
                     /translation="MRNLAEAFRRLARTTRFRLSAEGRKPHAYCCPVCGYRGPFADLV
                     QPQGVRLHARCPACRCLERHRLQALVLDGLDQRLFRNARILHLAPEPFLEQRFRTLSR
                     RYIAADR"
     gene            complement(326..1459)
                     /locus_tag="AC16VR_00100"
     CDS             complement(326..1459)
                     /locus_tag="AC16VR_00100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR6009576.1"
                     /translation="MVVAQRLGLPSQTWVARQLQTMNAFTPSVLTWETVAGTPPLGAP
                     VVELPFDPEPYAGAGRWMFRLRHAGDRNFFGALGAEARALSQELGALDPDVVLCHFGH
                     TALRLLPVARSLGIPLVAHFHGMDLAAMLDRWFYRSSLLRHFRHFDASIVVGSFQLRI
                     LEGLGLPTDQVHVIPCGVPTQMFVPGQRTEPGPIRFVSVGRLVEEKGMEECLLAFAKL
                     GQALGDAELTIVGEGPDRERLRALTDSLGITSRVTFAGALTPAAVAELLSGSDIYVQH
                     SHREGFGVALAEAASAGLPVVSTDVGGIPDQVVANETGFLIDVGDVAGMARAMARLGN
                     DPALRAQLGTAGRRRMVEKFDTRSQTGKLEAVLLSVLKDADRK"
     gene            complement(1508..>1979)
                     /locus_tag="AC16VR_00105"
     CDS             complement(1508..>1979)
                     /locus_tag="AC16VR_00105"
                     /inference="COORDINATES: protein motif:HMM:NF016908.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HIR6009577.1"
                     /translation="NRIGRAELRQGAADNRGTVSVDPRDDGTLAITTIDVAVAEMAGW
                     EPVVLIKIDVEGAEIDVLQGAEEIITRDMPLLCVEAHGPAQFAELARFLHSCGYGIVD
                     CLGGSPTYIADVTRTGDLKAVLSRRIWQLRSRMRGTWTRAAMRRLAIIVGPWQN"
BASE COUNT          358 a          668 c          608 g          345 t
ORIGIN      
        1 aatcggtccg ccgcaatgta ccgtcggctc aaggtacgga accgttgctc cagaaacggt
       61 tctggagcca ggtgcaggat gcgggcgttg cggaacagcc gctgatccag gccgtccagc
      121 accagagctt gcagccggtg ccgctccagg cagcggcagg caggacagcg agcgtgcagt
      181 cgcacgccct gaggctgcac caagtctgca aaaggcccgc ggtaaccgca taccgggcag
      241 cagtaagcgt ggggcttgcg tccctcggcc gacaaccgga accgcgtggt gcgggccagg
      301 cgccgaaatg cttccgccag gttcctcatt tccggtcggc gtccttcagc accgacagca
      361 gcacggcttc gagcttgccg gtctgggaac gcgtgtcaaa cttctcaacc atgcgtcggc
      421 gccccgctgt cccgagttgt gcgcgcagcg ccgggtcgtt tcctagccgc gccatggccc
      481 gcgccatgcc ggcaacgtct cctacgtcga tcagaaatcc ggtttcgttc gcaacgacct
      541 gatccgggat gccaccaaca tccgtgctga cgaccggcaa tcccgctgag gccgcttcgg
      601 ccagggcgac gccaaaaccc tcccggtgtg agtgctggac gtagatatcg gagccgctca
      661 gtagttctgc aacggctgcg ggcgtcaacg ctccggcgaa ggtcacccgg ctggtgatgc
      721 cgagcgagtc ggtcagagcc cttaagcgtt cccgatcggg accttcgccc acgatggtca
      781 gctcagcgtc gccaagggct tgccccagct tcgcgaaggc taacagacac tcttccatgc
      841 ccttttcctc taccaggcgc ccgacgctga cgaatcgtat tgggcccggc tcggttcgct
      901 ggccgggcac gaacatttgc gtgggcacgc cgcagggaat gacgtgcact tggtccgtgg
      961 ggaggcccag cccttcgaga atccgaagct gaaagctccc aacgacaatc gaggcgtcga
     1021 agtgccggaa atggcgcagt aggctggacc ggtaaaacca gcggtccagc attgcggcca
     1081 gatccattcc gtgaaagtgg gctaccaggg ggatgcccag agagcgtgca accggtagta
     1141 gtcgcagcgc ggtgtggccg aaatgacata gcaccacgtc cgggtccaga gcgccgagct
     1201 cctggcttag ggcccgggct tccgcgccca gggcgccaaa aaaattccgg tcccctgcat
     1261 gcctcaagcg gaacatccaa cggcctgcgc ccgcataagg ttctgggtca aagggtagct
     1321 ctaccaccgg cgcccctagc ggcggcgtcc ccgccaccgt ctcccaggtg aggaccgagg
     1381 gcgtgaacgc gttcattgtc tgcagttgcc tggctaccca ggtctggctg ggcaagccca
     1441 gacgttgcgc gaccaccacg acttttctcc ggccctgcac tgtgctcgga tttccctcag
     1501 gtactcgtta gttctgccac ggaccgacga tgattgccag gcgccgcatt gcggcccggg
     1561 tccaggtccc acgcattcgg cttcgtagct gccaaatcct gcgcgacagt accgctttta
     1621 gatctccggt gcgcgtcacg tcggcgatgt aggtgggcga tccgcccaga caatcgacga
     1681 ttccataccc gcacgagtgg aggaaccgcg ccaactcggc gaactgcgca gggccgtggg
     1741 cttccacaca cagaagcggc atgtccctgg ttatgatttc ctctgcgccc tgcagcacgt
     1801 cgatttccgc cccttccaca tcgatcttga tcagcaccac gggctcccag ccagccatct
     1861 ccgccactgc cacgtcgatg gtggtaatcg ccagggtgcc gtcatcacgg gggtccaccg
     1921 agacggtgcc gcgattgtcc gccgcaccct gacgcagctc ggcacgcccg atgcggttg
//
LOCUS       DBNHQR010000006         4378 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_443794, whole genome shotgun sequence.
ACCESSION   DBNHQR010000006 DBNHQR010000000
VERSION     DBNHQR010000006.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4378)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_443794"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /gene="ribD"
                     /locus_tag="AC16VR_00110"
     CDS             complement(<1..927)
                     /gene="ribD"
                     /locus_tag="AC16VR_00110"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HIR6009578.1"
                     /translation="MAADSDLVYLQSAVELAARGMYTCTPNPRVGCIIVRDGTVLGRG
                     WHVRAGEGHAEVNAIADAGGFVEGATVYVSLEPCAFHGRTPPCSDTLIEARVGRVVAA
                     MTDPHPEVAGRGFAALQQAGIPVEVIEMPQALELNAGYRCRIAKGRPLVRLKTAISLD
                     GRTGMASGESKWITSEAARADVQAWRAQSCAIVSGSGTIMADDASLTVRDERFRVDGA
                     IRQPLRAIADSRLELPADAAVFAGPGQTILFHGPDAEPHVPDIEHVCLGVATGADRGK
                     VDLGGMLAELGRRGCNEVLVEAGARLLGAFIRA"
     gene            complement(975..1433)
                     /gene="nrdR"
                     /locus_tag="AC16VR_00115"
     CDS             complement(975..1433)
                     /gene="nrdR"
                     /locus_tag="AC16VR_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HIR6009579.1"
                     /translation="MHCPFCGADDTKVIDSRLVAEGDSVRRRRECQTCGERFTTFETA
                     ELVMPRIVKSDGTREPFNEDKLRYGLQKALEKRPVSIEEIESALNRIMHRLRASGERE
                     LPSRDVGEAVMTELRTLDQVAYVRFASVYRDFQDLSEFQAEIQKLTFDLE"
     gene            complement(1468..2733)
                     /gene="glyA"
                     /locus_tag="AC16VR_00120"
     CDS             complement(1468..2733)
                     /gene="glyA"
                     /locus_tag="AC16VR_00120"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003340745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0006545 - glycine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIR6009580.1"
                     /translation="MFGKDQQIAGFDDEVAAAINEETRRQEEHIELIASENYASPRVM
                     EAQGGVLTNKYAEGYPRKRYYGGCEWVDKVEELAIERVKALFGAHYANVQPHSGSQAN
                     AAAYLALLEPGDTMLGMSLDAGGHLTHGAAPNFSGKVYNPVQYGLDNATGEVDYEEVA
                     RLAEEHRPRMIIGGFSAYSRRMDWQRFRDIADAVGAYLLVDMAHVAGLVAAGLYPNPV
                     PIADVTTSTTHKTLRGPRGGIILARENAEIEKKLNSALFPGTQGGPLMHVIAAKAVCF
                     KEAQGEEFVEYQRQVIANARVMAAGFQSRGYKVVSGGTDNHLFLLDLIDKEITGKAAD
                     AALGRANITVNKNAVPNDPRSPFVTSGLRIGTPAVTTRGFREAECETLTGWMCDVLDD
                     IDNDNMIGSVRQKVLDLCRRFPVYEASAA"
     gene            2880..>4378
                     /gene="ettA"
                     /locus_tag="AC16VR_00125"
     CDS             2880..>4378
                     /gene="ettA"
                     /locus_tag="AC16VR_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HIR6009581.1"
                     /translation="MAQYVYTMQGVSKTVPPKRTILENISLSFFPGAKIGVLGLNGAG
                     KSSLLRIMAGVDTEFEGEARPQKDIRVGFLPQEPKLDDSLDVRGNVEQGMGEIMSDLA
                     EYNEISDKFAEPMSEDEMAELLDRQGELATRIDAANGWDVDRTLEIAADALRLPPWDA
                     KVAHLSGGERRRVALCTLLLSKPDMLLLDEPTNHLDAESVAWLERFLAEYPGTVVAVT
                     HDRYFLDNVAGWILELDRGRGIPFEGNYSAWLEAKEKRLEMEASKEKARQRAIHSELE
                     WVRSNPKARQAKSKARLQRFEELMSKETQSRNETAELYIPPGERLGEKVIELSHVSKG
                     FDDRLLIDDLSVSIPQGAIVGIIGGNGVGKSTFFRMLTGQEAPDGGSIELGDTVELAY
                     VDQSRDALADQKTVWEEIADSQDVIRVGSYEIQSRSYVSRFNFKGPDQQKRVGDLSGG
                     ERNRVHLAKLLRRGANVLLLDEPTNDLDVETLRALEEALLNFPGTVLVIS"
BASE COUNT          743 a         1442 c         1451 g          742 t
ORIGIN      
        1 ggcgcggatg aacgcgccca gcagcctcgc gccagcttcc accagaacct cgttgcagcc
       61 ccggcggccg agctccgcca gcatcccgcc gaggtccacc ttgccccgat cggctcccgt
      121 cgcaaccccg aggcagacgt gctcgatatc gggcacgtgg ggttcggcgt ccgggccgtg
      181 aaagaggatg gtctggccgg gcccggcaaa gacggcggcg tccgcgggca gctccagccg
      241 ggaatcggcg atggcccgca gcggctgacg gatcgccccg tccacccgga accgctcgtc
      301 gcggacggtg aggctcgcat cgtctgccat gatggttccc gagccgctga cgatggcgca
      361 gctttgggcg cgccatgcct gcacgtcggc acgcgccgcc tcgctggtga tccacttgct
      421 ctcccccgac gccattcccg tgcgcccgtc cagggatatg gcggtcttga gcctgaccag
      481 ggggcggccc ttggcgatcc ggcagcgata gcccgcgttc agctccaggg cctgcggcat
      541 ctcgatgact tcaaccggta tgccagcctg ctgcagcgcg gcgaagcccc gcccggcgac
      601 ctcgggatgc ggatccgtca tggccgccac cacgcgaccg acccgcgcct cgatgagagt
      661 gtcgctgcac ggcggcgtgc ggccgtgaaa agcgcagggc tcgagggaaa cgtatacggt
      721 ggcaccttcc acgaagcccc cggcgtcggc gatggcgttc acctccgcgt ggccttcacc
      781 ggctcgcacg tgccagccgc ggccgagcac ggtgccgtca cggacgatga tgcatcccac
      841 acgtgggttc ggcgtgcacg tgtacatgcc tcgcgcggcc agctccacgg cgctttgcag
      901 gtagacgaga tccgagtcgg cggccatggc gcgcaaatat aggcgttcgc gtccgccgtg
      961 gctattcgcc cgggctattc caggtcgaag gtgagcttct gtatctccgc ctgaaactcg
     1021 ctcaagtcct gaaaatcacg gtaaaccgaa gcaaaacgca cgtaggccac ctgatcgagg
     1081 gtgcgcagct cggtcatgac agcctcgccc acgtcccggg acggaagctc acgctccccg
     1141 ctggcccgca gccggtgcat gatgcggttc aaggcgctct cgatctcctc gatgctgacc
     1201 ggacgtttct ccagcgcttt ctgcagaccg taacgcagct tgtcctcgtt gaacggctcg
     1261 cgggtgccgt cgctcttgac gatgcgcggc atgacgagct cggcggtctc gaaggttgtg
     1321 aatcgctcgc cacaggtctg gcattcgcgc cggcggcgaa ccgagtcccc ctcagccacc
     1381 agccgggagt cgatcacctt ggtgtcgtcg gcaccacaga aggggcaatg cacggcgtct
     1441 gcgcaggcga agccggggcc ttgcggctca ggccgcgctg gcctcgtaaa cggggaaccg
     1501 gcggcacaga tccagcacct tctgtcggac gctgccgatc atgttgtcgt tgtcgatgtc
     1561 gtcgaggacg tcgcacatcc accccgtcag ggtctcgcac tccgcttcgc ggaagccgcg
     1621 ggtcgtgacc gccggggtgc cgatgcgcag acccgaggtg acgaacggcg agcgggggtc
     1681 gttgggcacc gcgttcttgt tgaccgtgat gttggcgcgc cccagggcag cgtcagccgc
     1741 cttgccggtg atttccttgt cgatgaggtc gagcagaaag agatggttgt ccgtaccgcc
     1801 ggacaccacc ttgtagccgc ggctctggaa gccggcggcc atgacccgcg cattggcaat
     1861 gacctgacgc tggtactcca cgaactcctc gccctgagct tccttgaagc acacggcctt
     1921 ggccgcgatc acgtgcatga gcgggccgcc ctgggttccc gggaagagtg cggagttgag
     1981 cttcttttcg atctccgcgt tctcgcgggc caggatgatg ccgccgcgcg gtccacgaag
     2041 cgtcttgtgg gtggtggagg tggtcacgtc ggcgatcggt accgggttcg ggtagagacc
     2101 ggcagccacg agcccggcaa cgtgagccat gtccaccagc agataggccc cgacggcatc
     2161 ggcgatgtcg cgaaacctct gccagtccat cctccgggaa taggcggaaa acccgccgat
     2221 gatcatgcgg ggccggtgct cctcggcaag gcgggctacc tcttcgtagt ccacctctcc
     2281 cgtggcgttg tccaggccgt actgcaccgg gttgtagacc ttgccggaaa aattgggggc
     2341 agcgccgtgc gtgaggtggc cgccggcgtc caggctcatg cccagcatgg tgtcgccggg
     2401 ttcgagcagg gcaaggtagg cggccgcgtt ggcctgagat ccggagtggg gctggacgtt
     2461 ggcgtagtgc gcgccgaaca gcgctttcac ccgctcgatg gccagctctt ccaccttgtc
     2521 tacccactcg cagccgccgt agtaacgctt gcggggatag ccttcggcgt acttgttggt
     2581 cagcacgccg ccctgggcct ccatgacccg ggggcttgcg tagttctccg aggcgatgag
     2641 ctcgatgtgc tcttcctggc gtcgcgtctc ttcgttgatg gctgcggcaa cttcgtcgtc
     2701 aaacccggca atttgctgat ctttgccaaa catgggcgcc cccggtcgaa aacgcgcaag
     2761 tttaccccag ctcggcgcca tctcgtacgc aatcctttca tggggaaatt gaattgaacc
     2821 accgcggcga tttcggtagc ttgcgcgctc tctgagtcca tcgaccagga gcggcatcct
     2881 tggcccagta cgtctacacc atgcagggcg tctcgaagac ggtcccaccc aagaggacga
     2941 tcctcgagaa catctccctt tcgttctttc ccggggcgaa gatcggcgtg ctcggcctca
     3001 acggggccgg caagtcgagc ctgctcagga tcatggccgg cgtcgacacc gagttcgagg
     3061 gcgaggctcg cccccagaag gacatccggg tggggtttct tccccaggag ccgaaactcg
     3121 acgattccct ggacgtgcgc ggcaacgtcg agcagggcat gggcgagatc atgtcggacc
     3181 tcgccgagta caacgagatc tccgacaagt tcgccgagcc catgtccgag gacgagatgg
     3241 cggagctgct ggatcgtcag ggtgagctcg ccaccaggat cgacgcggcc aacggctggg
     3301 acgtggaccg gaccctggag atcgccgcgg acgccctgcg cctgccgccg tgggatgcca
     3361 aagttgccca cctctccggc ggtgagcggc ggcgggtggc tctgtgcacc ctgctgctct
     3421 ccaagccgga catgctcctg ctggacgagc cgaccaatca cctcgacgcc gaaagcgtgg
     3481 cctggctgga gcgcttcctc gccgagtacc cggggaccgt ggtcgccgtc acccacgacc
     3541 gctatttcct agacaacgtc gccggctgga tcctggagct cgatcgcggc cgcggcatcc
     3601 cgttcgaggg caactacagc gcatggctgg aagccaagga gaaacggctg gagatggaag
     3661 cgtccaagga gaaagcccgc cagcgggcga tccactctga gctcgagtgg gtgagatcga
     3721 accccaaggc ccggcaggcc aagagcaagg ctcgcctgca gcgcttcgag gagctcatgt
     3781 ccaaggagac ccagagccgc aacgaaaccg ccgagctcta catcccgcca ggggagcggc
     3841 tgggggaaaa ggtcatcgag ctgtcccacg tgagcaaggg cttcgacgac cggctgctca
     3901 tcgacgatct gagcgtatcc atccctcagg gcgccatcgt cggcatcatc ggcggcaacg
     3961 gggtgggcaa gtcgaccttc tttcgtatgc tgaccgggca ggaggcgccg gacggcggga
     4021 gcatcgagct gggcgacacc gtggagctgg cctacgtgga ccagagccgg gacgccctgg
     4081 ccgaccagaa gacggtatgg gaggagatag ccgacagtca ggacgtcatc cgcgtgggaa
     4141 gctatgagat ccagtcgcgc agctacgtct cccggttcaa cttcaaaggt ccggaccagc
     4201 agaagcgggt cggcgatctc tcgggcggcg agcgcaaccg cgtgcatctg gccaagctgc
     4261 tgcgccgcgg cgcgaacgtg ctgctgctgg acgagccaac caacgacctg gacgtggaga
     4321 ccctgcgcgc gctggaggaa gcgttgctga actttccggg aaccgtcctg gtgatatc
//
LOCUS       DBNHQR010000007         5260 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_63608, whole genome shotgun sequence.
ACCESSION   DBNHQR010000007 DBNHQR010000000
VERSION     DBNHQR010000007.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5260)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5260)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5260
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_63608"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="AC16VR_00130"
     CDS             <1..984
                     /locus_tag="AC16VR_00130"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6009582.1"
                     /translation="RFDAQGVLTWVYCEEDFPQGNPCGKRNLIEIPCDTWAGFIWYNM
                     DPDCKPLADFLDPVARQLDNYRMEDMRRTHWVTLEGNFNWKCVQDNFNESYHLPYVHP
                     QSCISLDEHYSSCQFDLYPSGHCRMFMPGGGPNSNYQGSPEAVFERLGQDLAFWEIDP
                     EPFRDDLAGLRTALQQQKRKLAQKKGYDFSRYSDEQLTDNYHYTLFPNLSFSMKPDGC
                     IFLRGNPHPTDPEKCLFDMWYLTLFPEGAKEYYSNSMRDWVSVETPAAHERGKVGEVS
                     CGPGIDQDVAIWSTQQKGLTSRGFRGEYMPTQERRIRFFHENIDRYINGQL"
     gene            993..1355
                     /locus_tag="AC16VR_00135"
     CDS             993..1355
                     /locus_tag="AC16VR_00135"
                     /inference="COORDINATES: protein motif:HMM:NF025178.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HIR6009583.1"
                     /translation="MSEAKQINLGPLSDLEEGDKRSFDAEGTQVLVCRVRGELYAIED
                     LCSHANKPLCRGRLSGYMITCPVHSARFDIRDGAHQGPPAFTGIRTFPIEEGADGAIV
                     TVTPKPLPAGFGGPLMTR"
     gene            1367..1861
                     /locus_tag="AC16VR_00140"
     CDS             1367..1861
                     /locus_tag="AC16VR_00140"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SixA phosphatase family protein"
                     /protein_id="HIR6009584.1"
                     /translation="MKIWIIRHAKSSWAQPGLSDFDRPLNDRGRADGPRMAAWLASQD
                     APAEWIWTSDAARARATAAFVERGFSAARPLVVEEHALYHASARGALDVLRGTPEAVA
                     SVALVAHNPGLTDLVNLLAGNLVTENLPTFGVARFDVAAPWHSVGEQRADLEILTAPK
                     EIPS"
     gene            2099..2290
                     /locus_tag="AC16VR_00145"
     CDS             2099..2290
                     /locus_tag="AC16VR_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009585.1"
                     /translation="MVFLCFIVLLGIAYLVWRVTDQMPDLIFRLSEVQRDVAEIRRRL
                     TEESDAPAGAAQGGGDAEA"
     gene            2340..2915
                     /locus_tag="AC16VR_00150"
     CDS             2340..2915
                     /locus_tag="AC16VR_00150"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6009586.1"
                     /translation="MDPLAQLTRDRAEARRLEDPCANLCTVASVDSAGNPQARTLVLR
                     DVNERLAIFANETSPKWREISLNERISVVVWLPVLNLQYRLRCGTERVPTHLVHDRWH
                     QRPDPPKRLDWFYTNHLPQSSPLPDRDTLLERLAQIDLPEPLAPPQTAAGLYLSPTTV
                     ERLDLNQSNGIHDRRHFELSEDGWVQSVLVP"
     gene            complement(2948..3874)
                     /locus_tag="AC16VR_00155"
     CDS             complement(2948..3874)
                     /locus_tag="AC16VR_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004345413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR6009587.1"
                     /translation="MSHLELRHLRTLVALRDTGSLVEAAERVFLTQSALSHQLKDLES
                     RLGVTLFIRKTRPVRFTTAGRRLLKLADQVLPHVHGAEHDLERLAGGESGRIYMAIEC
                     HSCFEWLLPAINAYREEWPDVELDIASGFHFAPLPALARGDLDLVITADPIEELGLVY
                     LPLFSYEAQLAVARDHELADKPWVEPADLSREVLICYPVDRSRLDIFTSFLDPANVEP
                     ARVRNAELTTMMIQLVASGRGVACLPNWALHEYRQRDYVQVKSLGEDGVWPTLYAAVR
                     ADQADAAFIRGFIDVAKQTCFQNLVGIVTADV"
     gene            4037..4948
                     /locus_tag="AC16VR_00160"
     CDS             4037..4948
                     /locus_tag="AC16VR_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009588.1"
                     /translation="MFNPNPIVVEAFAAHCTNAYVEAFANTLAQPREDHLETLREAVS
                     TALGLLARCDCPYHNVEHTIMVADAGLAILKGQLALEDPISPDDWLHAVVAMLFHDVG
                     YLRDLLPEDGPEGCLVEHGGRRVSPPADATDAWMMPYHVTRGDLFVRQHYADHPLIDV
                     DRVAAYIEMTRFPVPDRPPYQQLETLGALVRAADLIGQMGDPNYLAKLSSLFVEFKEN
                     GEADRLGFASVAELRADSQAFFSQQISPYLPKALKLLRATEEGRAWVVSLYENIERRA
                     QAGGSAAQPDPSKQENSRAGRIHAGNG"
     gene            5004..>5260
                     /locus_tag="AC16VR_00165"
     CDS             5004..>5260
                     /locus_tag="AC16VR_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009589.1"
                     /translation="MSALLGAGMSELATYEFGQEAPSGRRPLRIALLGYRSNPFSGGQ
                     GVYLKFLSRALVDAGHEVDVISGEPYPELDGRVGLITLPGL"
BASE COUNT          984 a         1750 c         1634 g          892 t
ORIGIN      
        1 cgcttcgatg ctcagggtgt cctcacatgg gtctactgcg aggaggactt cccccagggc
       61 aacccctgcg gcaagcgcaa tctgatcgag atcccgtgcg atacgtgggc ggggttcatc
      121 tggtacaaca tggaccccga ctgcaaacct ctcgccgatt ttctcgaccc ggttgcccga
      181 cagctcgaca actatcgtat ggaagacatg cgccgaaccc actgggtgac cctcgagggc
      241 aacttcaact ggaagtgcgt ccaggacaat ttcaacgaga gctatcacct gccatacgtg
      301 cacccgcaat cgtgtatcag cctggacgag cactactcga gctgccagtt cgatctctac
      361 cccagcggtc actgccgcat gttcatgccc ggtggcgggc cgaacagcaa ctatcagggc
      421 tccccggagg ccgtgttcga gcgcctcggt caggacctgg cgttctggga gatcgacccg
      481 gaacccttcc gagacgacct cgccggtttg cggaccgcgc tgcagcagca gaagcgcaag
      541 ctggcccaga agaagggcta cgacttctcg cgatattcgg acgagcagct cacggacaac
      601 taccactaca cgctgtttcc gaacctctcg ttcagcatga agcccgacgg gtgcatcttc
      661 ctgcgcggca acccacaccc caccgatccg gagaagtgcc tgttcgacat gtggtacctg
      721 accttgtttc cggagggcgc caaggagtac tactccaact ccatgcgcga ctgggtgtcc
      781 gtcgagaccc ccgccgccca tgagcgcggc aaggtcggcg aggtgtcctg cgggcccgga
      841 atcgatcagg acgttgccat ctggtcgact cagcagaaag gcctgacgtc tcgcgggttc
      901 cgcggcgagt acatgcccac ccaggaacgc cgcatacgct ttttccacga gaatatcgac
      961 cgttacatca acgggcagct gtgagcgagg cgatgagcga agcaaagcaa atcaacctgg
     1021 gtcctctgag cgacctggaa gaaggcgaca agcgctcctt cgacgcggag ggtacgcagg
     1081 tcctcgtctg ccgcgtccga ggcgagctct acgccatcga ggatctctgc agccacgcca
     1141 acaagccgct ctgccggggc aggctttcgg gctacatgat cacctgcccc gtccacagcg
     1201 cccgtttcga catcagagat ggcgcccatc aggggccacc ggcgttcacc ggcattcgca
     1261 cgtttcccat cgaggaggga gcggacggcg cgatcgtgac ggtgacgccg aaaccgcttc
     1321 ctgccggctt cggcggcccc ttgatgaccc gctgaacgcc ggccccgtga agatctggat
     1381 catccgtcac gccaagtcat catgggcgca gccaggtctg tccgacttcg atcggccact
     1441 caatgaccgc ggccgggccg acgggccccg catggccgcc tggctcgcaa gccaggacgc
     1501 gcccgcagag tggatctgga ccagcgacgc cgcaagggcc cgagccaccg cagctttcgt
     1561 cgagcggggc ttctcggccg cacgcccgct ggtcgtagag gagcacgccc tgtatcacgc
     1621 cagcgcgcgc ggcgccttgg acgtgctgag aggcactccg gaagcggttg ccagcgtggc
     1681 gctggtggcc cacaaccctg gtctcaccga cctggtcaac ctgctggccg gcaacctggt
     1741 gacggagaat ctgccgacct tcggcgtggc gcgcttcgac gtcgcggccc catggcatag
     1801 cgtaggggag cagcgcgcag atctggaaat cctgacggcg ccaaaggaaa ttccgtccta
     1861 gcctggtttg tcgatcctga acaggaagca accgttgcag agctcgacgg gaacgactcg
     1921 atagcgcacc agatttcatg acccgcgacg cggaggcaat cagcccacgc tgcatacgag
     1981 cggtgaaacg gttgcgctgg aaacccaaag ggcagccgcg ttcacgttca tccgctggac
     2041 tcccggcgat ggagtcggta tacctaatcg cgagtccccc aaccaaccgg agatgtgcat
     2101 ggtcttcctt tgctttatcg tgctgctggg catcgcctac ctcgtgtggc gcgtcaccga
     2161 tcagatgcca gacctgattt tccgcctgag cgaagtgcag cgcgacgtcg cggagatccg
     2221 ccgccgcctg accgaggaga gcgacgcacc ggccggggcc gctcagggcg gaggcgacgc
     2281 cgaggcctga cgtcggcaag ccggcgcccg ccatcgaacg aagcaccacc gggcttttag
     2341 tggacccgct tgcccaactc acccgcgacc gcgcggaggc gaggcgcctg gaagacccct
     2401 gtgccaacct ctgcacggtg gcttccgtcg actccgcagg aaatccccag gcacggacgc
     2461 tggtgctgcg cgacgtcaac gaacgcctgg cgattttcgc aaacgagacg agccccaaat
     2521 ggcgggaaat cagcttaaac gagcggattt cggtggtggt ctggctgccg gtcctgaacc
     2581 tgcaataccg cctgcggtgc ggcaccgagc gcgtgccgac gcacctcgtg catgaccgct
     2641 ggcaccagcg cccggatccg cccaagcggc tggactggtt ctacaccaac caccttccgc
     2701 agagttcccc actgcccgac cgggacacgc tgctggagcg cctggcccag atcgatcttc
     2761 ccgagccgct ggcacctccg cagacggcgg ccggactcta cctgtcgccg acgacggtcg
     2821 aacgcctgga tctcaatcag agcaacggca ttcacgaccg ccggcacttc gagctgtcgg
     2881 aagacggctg ggtgcagtcg gtgctggttc cctgatcacc gccaccgcga ccaggtcgtc
     2941 gatcctgtca gacgtccgcg gtgacgatac cgaccaggtt ctggaagcac gtctgcttcg
     3001 caacgtcgat gaagcctcga atgaaggcgg cgtccgcctg atccgcccgg accgccgcgt
     3061 agagcgtggg ccagacgccg tcctctccca ggctcttcac ctgaacgtag tcgcgctggc
     3121 gatactcatg cagagcccag ttgggcaggc atgccacgcc gcgcccgctg gccaccagct
     3181 ggatcatcat ggtcgtcagc tccgcgttcc gcacacgagc cggctcgacg ttggccggat
     3241 cgaggaagct ggtgaagatg tcgaggcggc tgcggtccac cggataacag atgagcacct
     3301 cgcgcgacag atcggcgggc tccacccagg gtttgtccgc cagctcgtga tcgcgggcaa
     3361 ccgcgagctg cgcttcgtag ctgaacagcg gcaggtagac gagcccgagc tcctcaatgg
     3421 ggtccgcggt gatcaccaga tcgagatcgc cgcgagcgag cgcggggagc ggagcgaagt
     3481 gaaagccgct ggcaatgtcc agctcgacat ccggccactc ctcgcgatag gcattgatgg
     3541 cgggcagcag ccactcgaag cagctgtgac actcgatggc catgtatatc cttcccgact
     3601 cgccgccggc cagcctctcc aggtcgtgct cggcgccgtg gacgtggggc aacacctggt
     3661 cggcgagctt gagcagtcgg cggccggcgg tggtgaagcg aacgggtcgc gttttgcgga
     3721 tgaacagcgt gacgccgagt cgggactcca ggtccttgag ctggtgggaa agcgcggact
     3781 gggtcaggaa aacccgttcc gccgcctcga cgaggctgcc cgtgtcccgc agcgctacga
     3841 gggtcctcag gtgcctgagc tccagatggg acatgaattt ccctcacgaa attctttcct
     3901 taagtgcaga atattcataa aacgacgagg cctcaatctc aatctcccgg tacccgatat
     3961 ggcatagaat gaggactgag gcacgcaggc tggggggacc gtgccttaaa ctgcctcgaa
     4021 ttgtccggac tgcttgatgt tcaacccgaa tccaatcgtc gttgaagctt ttgccgcaca
     4081 ttgcacgaac gcttatgtcg aggcgttcgc gaacacgctc gcgcagccgc gcgaagatca
     4141 tctcgaaacc ctgcgggagg ccgtctccac ggccctggga ctgctcgcac gctgcgactg
     4201 cccctaccac aacgtcgagc acaccatcat ggtggcggac gccgggctgg cgatcctgaa
     4261 agggcagctc gccctggaag atcccatctc gccagacgac tggcttcatg cggtggtcgc
     4321 catgctcttt cacgacgtcg gctatctgcg ggacctcctg cccgaggacg ggccggaggg
     4381 ctgcctggtg gaacatggcg gcaggcgggt atcgcccccc gcagacgcca cagatgcctg
     4441 gatgatgccg taccacgtca cccgcggtga tctcttcgtt cgccaacact atgcagacca
     4501 tccgctgatc gacgtcgatc gcgtggccgc ctacatcgaa atgacgcgct tcccggttcc
     4561 cgaccggcca ccctaccagc agctggaaac cctgggcgcg ctcgtgcggg cagccgatct
     4621 gatcggccag atgggcgacc cgaactatct ggcaaaactg tccagcctgt tcgtggagtt
     4681 caaggaaaac ggcgaggctg accggctcgg gttcgcgagc gtggccgagc ttcgcgcgga
     4741 ttcccaggct tttttcagcc agcagatttc accctacctg cctaaagcgc tgaagctgct
     4801 tcgcgcaacc gaagagggcc gggcctgggt cgtcagcctg tacgagaaca tcgagcggcg
     4861 ggcccaggcg gggggctctg cggcccagcc tgacccatca aagcaggaga attcccgcgc
     4921 tggccgtatc cacgcgggca acggctgacc ctaccctcat ccgccgtcca acgcctggca
     4981 ctccttcgcg ccgctgatta gacttgtccg ctttgctcgg ggcaggcatg tcggagctcg
     5041 cgacctacga gttcggccag gaagcgcctt ctggtcggcg gccactgcga atcgcgctgc
     5101 tggggtatcg cagcaacccg ttcagtggcg ggcagggcgt gtatctgaag ttcctctccc
     5161 gcgccttggt cgatgccggg cacgaggtgg acgtgatatc cggcgagccc tacccggaac
     5221 tggacgggcg cgtcgggctg atcacgctgc caggcctgaa
//
LOCUS       DBNHQR010000008         4970 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_190513, whole genome shotgun sequence.
ACCESSION   DBNHQR010000008 DBNHQR010000000
VERSION     DBNHQR010000008.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4970)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4970)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4970
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_190513"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..758
                     /gene="cmoA"
                     /locus_tag="AC16VR_00170"
     CDS             30..758
                     /gene="cmoA"
                     /locus_tag="AC16VR_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003124239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HIR6009590.1"
                     /translation="MSSRDRVYRESKRQIVDFAFDEAVADVFPDMIRRSVPGYETVIP
                     ITGMLAARHLEHMPDERRLAYDLGCSLGATTTAVLDQLDERPARVIAVDNAAAMIARA
                     VDAIRDPRARFEVADVTMLEFEPAGVVLMNFVLQFIDPTQRVPLLKRIRQALEPDGLL
                     IVSEKLRFEDPYTQALFERAHLDYKRANGYSELEISQKRSALEKVMIVDTEAEHRSRF
                     EAAGFATTNRWFQCLNWVSYLVAP"
     gene            755..1690
                     /gene="cmoB"
                     /locus_tag="AC16VR_00175"
     CDS             755..1690
                     /gene="cmoB"
                     /locus_tag="AC16VR_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HIR6009591.1"
                     /translation="MIFEPVLDLIPEWRRLGPDLNARLSTKGHGDYPRWEAALAALPD
                     LQAEQAALSDRVTVSGNASEDQRRDLRESLMALHPWRKGPFSLFEVDIDCEWRSDWKW
                     LRVLPHLDSLSGRRVLDVGCGNGYYGWRMLEAGATAVFGVDPTLVFCMQHLAVSRYLS
                     GAANWVLPLRFEQLPPHPFDTVFSMGVIYHRRDPAEHARRLAEFTRPGGQVVVESLVV
                     EHGRSLKPVGRYARMRNVHVVPTTKRLARWLKDAGFVDPELVDLTPTTVAEQRATTWM
                     RFESLEDALDPKMRNRTIEGHPAPVRAVMVARKPV"
     gene            1712..2143
                     /locus_tag="AC16VR_00180"
     CDS             1712..2143
                     /locus_tag="AC16VR_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009592.1"
                     /translation="MRFETAYGLKTAKPQNTTMMLGIIPISFRFRGKIMRLEDMYHCF
                     REHPGTGLHPTQISRYTGLGLAEVNARLEATPELFVRLPKRPDGITRYRLTSATTAKA
                     PEDVEKFLRQAARRESLMLYAVGSMVLLILLIVVILVGPAV"
     gene            2144..3253
                     /locus_tag="AC16VR_00185"
     CDS             2144..3253
                     /locus_tag="AC16VR_00185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6009593.1"
                     /translation="MPELLDDKQRALREHAATFAREQLEPRRSMDNKQAREEIVRAAK
                     AAGFYAMTQPAEFGGNPASVLELTIVRDELAASNAPHADAVFGPGPGVLAGVGEPLAS
                     DYLGPVLAGTKQGAFGFTEPDDAPGPTSGRLESDELIVSGQKSYVTGGEQADFINTLV
                     QIEGHGPALVVIDTHAAGVIRERTFSSLDGSRHAAFRFEEVRVPRSHVIGKPGEGLPR
                     AMRQIGDTRLAIAAQCVGAMRFVLELLTTHLKAPDRSGRARGSKEGVRLRYADLRIKA
                     YAARSMVYRTARLADSGSNVINEGIACKVFATETAGEIVDTGIQLVGGSALVESHPLA
                     ELYRRLRALRLAEGGSDVLRLNLARGRLDLDKGRI"
     gene            complement(3278..3880)
                     /gene="smrA"
                     /locus_tag="AC16VR_00190"
     CDS             complement(3278..3880)
                     /gene="smrA"
                     /locus_tag="AC16VR_00190"
                     /inference="COORDINATES: protein motif:HMM:NF033154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HIR6009594.1"
                     /translation="MGDEDVFREAMSDVAPLQRKQRAQPERGPVEATSAQLERREFAL
                     GNRDREAADPNYLTLGEVPQRQPREIVEWKKDGVQNAVFARLKAGKYPVEGSVDLHRQ
                     TVREARAAVFRFLNLAMAKGWRMVLIAHGRGEQSETPARIKSYVAAWLAQVPEVIAYH
                     SAQNNQGGTGACYVLLKKSPQARENNREQYGGKGEAEEPG"
     gene            complement(3888..4661)
                     /locus_tag="AC16VR_00195"
     CDS             complement(3888..4661)
                     /locus_tag="AC16VR_00195"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIR6009595.1"
                     /translation="MESGSSEIDRLEPRAFERDGFVVVRGCCDPGLVRRIRDRASSYL
                     QPLLGPAEFEADVGYPGSPASRASDGGDTCRRLLHAYARDSVFRDWATGSSVGAVLRV
                     LFGGADVMMSQCHHNCVMTKHPGYSSATLWHQDIRYWSFDRPELVSVWLALGEETEEN
                     GALKVIPGSHAMNIDRGRLDRDLFLRPELAENAELVEHAQTVPLEAGDVLFFHCRLFH
                     AAGKNLTDEVKLSVVTTYHTADNRPIPDTRSAQYPGVPL"
     gene            complement(4669..>4970)
                     /locus_tag="AC16VR_00200"
     CDS             complement(4669..>4970)
                     /locus_tag="AC16VR_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR6009596.1"
                     /translation="PLGLYYFGDDREEEGKVLSHTTSGGVTVNDVVMHVAQEDLPFGG
                     VGPSGMGAYHGHDGFKTFSHAKSIFTQSKVNVAELAGLRPPYGEKMLKAIRAQMR"
BASE COUNT          869 a         1679 c         1580 g          842 t
ORIGIN      
        1 cttcgtgttg ccgaaggggg gccgcctcct tgagttcccg cgaccgcgtc taccgcgaat
       61 caaaaagaca gatcgtcgac ttcgccttcg acgaagccgt ggccgacgtg tttcccgaca
      121 tgattcgccg ctccgtcccc ggctacgaaa ccgtgatccc gatcacggga atgctggcgg
      181 ccaggcatct cgagcacatg cccgacgagc gccgtctggc ttacgacctg ggttgctcgc
      241 tgggcgccac caccaccgcc gtcctggacc aactggacga acggcccgct cgcgtcattg
      301 ccgtggacaa cgctgcggcc atgatcgccc gagcggtcga cgccatccgt gacccgcggg
      361 cgcgcttcga ggttgccgac gttaccatgc tggagttcga acctgccggc gtcgtgctga
      421 tgaacttcgt gctccagttc atcgacccca cgcaacgggt gccactcctg aaacgaatca
      481 ggcaagcgct cgaaccagac ggcttgctca tcgtctccga gaagctgcgc ttcgaagatc
      541 cttacaccca ggccctgttc gagcgggccc acctcgacta caagcgtgcc aacggatact
      601 ctgagctcga gatcagccag aaacggtcag cactggaaaa ggtgatgatt gtcgacaccg
      661 aagcagagca tcgcagccgc ttcgaagccg ccggattcgc aaccaccaac agatggttcc
      721 agtgcctgaa ctgggtttcc tacctcgttg ccccatgatc tttgagcccg tcctggatct
      781 catacccgaa tggcgccggc tcggcccgga cttaaacgcg cgcctgtcga cgaaggggca
      841 cggcgactac ccgcgctggg aagccgcgct cgcagcgctg cccgacctcc aggcagagca
      901 ggccgccctc agcgatcggg taacggtgag cgggaacgcc tccgaagacc agcggcgcga
      961 cctgcgggaa tcgctgatgg ccctgcatcc gtggcgcaag ggacccttca gcctgttcga
     1021 agtggacatc gactgcgagt ggcgctcgga ctggaagtgg ctgcgcgtgc tcccccacct
     1081 ggattccctt tccgggcgcc gcgtcctcga cgtgggatgc ggcaacggct actacggctg
     1141 gcgcatgctg gaagccggcg ccaccgcggt tttcggcgtg gatcccacgc tggttttctg
     1201 catgcagcat ctggcagtca gccgctacct gtccggcgcg gccaactggg tgctgcccct
     1261 gaggttcgag cagcttcctc cgcatccgtt cgataccgtt ttcagcatgg gcgtgattta
     1321 tcaccgacgg gatcccgccg agcacgcccg ccggcttgcg gagttcacga ggcccggtgg
     1381 ccaggtcgtg gtggaatcac tcgtggtcga gcacggccga tctttgaagc ccgtaggccg
     1441 ctacgcgcga atgcgcaacg ttcacgtggt gcccacgacg aaacggcttg cgcgctggct
     1501 caaggacgcg ggcttcgtcg atccggagct cgtcgacctg acacctacca cggtcgccga
     1561 gcagcgcgcg acgacgtgga tgcgattcga atccctcgag gacgctctag atcccaagat
     1621 gcggaaccga accatcgaag gtcatcccgc ccccgtccgg gccgtcatgg tcgcgcgcaa
     1681 gccggtctga ttcgcgccgg cgggcgtgga aatgcgcttt gaaaccgcgt acggtctcaa
     1741 gacggcaaag ccgcagaaca caaccatgat gctcgggatc attcccatca gttttcgctt
     1801 ccgcggcaag atcatgcggc tggaggacat gtatcactgc tttcgggagc atcccggaac
     1861 cgggctgcat ccgacgcaga tatcccgcta tacgggcctg ggtctggccg aggtgaacgc
     1921 cagactggaa gcgactcccg agctcttcgt gcgccttccg aaacgccccg acggcataac
     1981 gcgctaccgg ctgaccagtg ccaccacggc aaaggcgccc gaggacgttg agaagttcct
     2041 ccgccaggcg gccaggcgcg aaagcctgat gctttacgcc gtgggaagca tggtgctgtt
     2101 gattctgctc atcgtcgtca tcctcgtcgg gcctgccgtc tagatgcccg aactgctcga
     2161 cgataagcag cgcgcgcttc gcgagcacgc cgcgaccttc gcccgtgagc agctggaacc
     2221 caggcgcagc atggacaaca agcaggcgcg tgaggaaatc gtccgcgctg ccaaagccgc
     2281 cggtttctac gccatgaccc agcccgcgga gttcggcggc aacccggcgt cggttctgga
     2341 actcacgatc gtcagagacg agttggcggc gagcaacgcg ccccacgccg atgcagtgtt
     2401 cgggccgggt ccgggggtgc tggccggagt gggagagccc ctggctagcg actaccttgg
     2461 gcccgtgctg gcgggcacca agcaaggcgc gttcggtttc accgagcccg atgacgcccc
     2521 cggaccaacc agcgggcggc tggaatcaga cgagctgatc gtcagcgggc agaagtcgta
     2581 cgtcaccggc ggcgagcagg ccgacttcat caacaccctc gtgcagatcg agggccacgg
     2641 ccccgcgctg gtcgtgatcg atacccatgc cgccggggtg atccgcgagc gaaccttctc
     2701 gtcgctagac ggcagccgtc acgccgcctt tcgattcgaa gaagtgcgcg tgccccgctc
     2761 gcacgtgatc ggcaagcccg gcgaggggct gccgcgagcc atgcgccaga tcggcgatac
     2821 gcgcctggcc atcgccgccc agtgcgtggg ggccatgcga ttcgttctcg aactgctcac
     2881 gacgcacctc aaggcgcccg accgctccgg ccgggcgcgc ggcagcaagg aaggcgtgcg
     2941 gcttcgctac gccgatctgc gcatcaaagc gtatgccgcg cgcagcatgg tttaccggac
     3001 ggccaggctc gcggactccg ggtccaacgt gatcaacgag gggatcgcct gcaaagtgtt
     3061 tgcaacggaa accgccggcg agatcgtcga caccgggatc cagctggttg gcgggagcgc
     3121 gctggtggag tcccacccgc tggccgagct ctaccgacgg ctgcgcgcgc tgcggctggc
     3181 agagggtggc agcgacgtgc tgcgactgaa cctggcgcga gggcgcctgg acctggacaa
     3241 aggccggatc tgaaaatgcc cgcccgcggg cacagcttca gccgggttcc tcggcttccc
     3301 ccttgccgcc atattgctcg cgattatttt cacgggcctg aggggacttc ttcagcagca
     3361 cgtagcaggc gccggtaccg ccctggttgt tctgggcgct gtgataggcg atcacctcgg
     3421 gcacctgagc aagccaggcg gccacgtagc ttttgatacg cgccggggtc tcgctctgct
     3481 cgccgcggcc gtgggcgatg agcaccatgc gccagccctt cgccatggcg agattgagaa
     3541 accggaacac ggcggctcgg gcttccctca ccgtttgccg gtgcagatcc acagaaccct
     3601 ccaccggata ctttcccgcc ttcagccgcg cgaagacggc gttctgcaca ccgtccttct
     3661 tccactcgac aatctcccgc ggctgccgct ggggcacctc gccaagggtc aggtaattcg
     3721 ggtccgcggc ctcgcggtcc cggttgccga gggcgaattc gcggcgttcg agctgcgcgg
     3781 aggtcgcctc gaccggccct cgctcgggtt gcgcgcgctg cttgcgctgc agcggcgcaa
     3841 cgtcgctcat ggcctcgcga aagacgtcct cgtcgcccat gattcggcta cagcggcact
     3901 cccggatact gcgcagaccg ggtatccggg atcggccggt tgtcggcggt gtggtacgtg
     3961 gtcaccacgg aaagcttcac ctcgtccgtg agattcttgc ctgccgcgtg gaacaaccgg
     4021 cagtgaaaaa acagcacgtc gcctgcttca agcgggaccg tctgggcatg ctcaacgagc
     4081 tcggcgttct cggcgagctc ggggcgcagg aaaagatccc ggtcgagccg gccccgatcg
     4141 atgttcatgg cgtggctgcc cggaatgacc ttgagcgcgc cgttttcctc cgtctcttcg
     4201 cccagcgcga gccagacgga gacgagctcg gggcggtcga acgaccagta ccggatgtcc
     4261 tgatgccaca gcgtcgcgct gctgtagccg ggatgcttgg tcatcacgca gttgtgatga
     4321 cattgcgaca tcatcacgtc cgcgcccccg aaaagaacgc ggagaacggc ccccacggag
     4381 cttcctgtgg cccagtccct gaaaacagaa tctcgagcgt aggcatgcag gagacggcgg
     4441 cacgtatcgc ccccatccga agcgcggctc gcaggggatc cgggataccc cacgtcagct
     4501 tcgaactctg cgggccccag caggggctgg agataggaag aggcgcgatc gcgaatccgc
     4561 ctcaccagcc ccgggtcgca gcagccgcgc accacgacga agccgtcgcg ctcaaaagcg
     4621 cgcggctcga ggcggtcgat ctcgctgctg ccggattcca agatacgctc aacgcatctg
     4681 ggcgcggatg gccttgagca tcttctcgcc gtagggcgga cgcaggccgg ccagctcggc
     4741 cacgttgacc ttgctctggg tgaagatcga cttggcgtgg ctgaatgtct tgaagccgtc
     4801 gtgcccatgg taggcgccca tgcccgacgg ccccaccccg ccgaacggca gatcctcctg
     4861 agccacgtgc ataaccacgt cgttcaccgt caccccgccc gaggtggtgt gcgaaaggac
     4921 ctttccttcc tcttcgcggt cgtcgccgaa gtagtacagt cccagcggcc
//
LOCUS       DBNHQR010000009         3170 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1584487, whole genome shotgun sequence.
ACCESSION   DBNHQR010000009 DBNHQR010000000
VERSION     DBNHQR010000009.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1584487"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="AC16VR_00205"
     CDS             complement(<1..529)
                     /locus_tag="AC16VR_00205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MsnO8 family LLM class oxidoreductase"
                     /protein_id="HIR6009597.1"
                     /translation="MPRFSILDLAPITIGGSARQALHNARDLARHGEAWGFERFWLAE
                     HHNMPGIASSATAVAIGFVAEGTSTIRVGAGGIMLPNHAPLVIAEQFGTLEALYPGRI
                     DLGLGRAPGTDQITAHALRRTLNSDVDGFPQDLMELMLYLGDPVEGQRVHAVPGAGSK
                     VPVWILGSSLFGAEVA"
     gene            complement(545..937)
                     /locus_tag="AC16VR_00210"
     CDS             complement(545..937)
                     /locus_tag="AC16VR_00210"
                     /inference="COORDINATES: protein motif:HMM:NF013304.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HIR6009598.1"
                     /translation="MGDYVYPGILSSLALLVYYFTLFKAGMARGKYNIPAPSHEGPEE
                     YVRRVRVHQNTMEHLVLFLPGLWLFSYAVDPIWAAGIGVLWPVGRLWYAFAYYEAAEK
                     RGPGLYLSMPPIYIFVIGSLIGFMLELF"
     gene            complement(992..2323)
                     /locus_tag="AC16VR_00215"
     CDS             complement(992..2323)
                     /locus_tag="AC16VR_00215"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6009599.1"
                     /translation="MVTNARDWRSEPAPAWAQPDVPAHHIEGSRYTDRGFAAAEWDGM
                     WSRVWLLLGRAREMPNPGDYQVEDVGPESFIMVRQDDGSIRAFYNVCQHRGSRLIFNA
                     AGSVERISCPYHSWTYGRDGALLVVQDPEDFSSGNPCERLSLVEVRCETFAGWVWVNM
                     DPDCVSLREYLGPVWEDWQTWQPEDWRRSLAISAWVPCNWKVIQDNFCESYHLLTVHP
                     QLTANIEEGVPWTRFDMANEGHNRMIMQAGMPSHRQAQGPKLVEPLLSQMAMWELDPD
                     DFAGREHDTRAAIQQQMRDLGAPRGYAHYDRLRDEQLTDPHHYNIFPNCSVTFGADSV
                     LLQRMRPHPTDPQQCRFDHWTYVAPAAIEHGVFRNSDGRVEFTGDAEIKEVEYGKESM
                     GPIADQDIGITTGQQLGLRSRGYRGANLADQESRIARFHGVIDEYIAGERP"
     gene            complement(2339..>3170)
                     /locus_tag="AC16VR_00220"
     CDS             complement(2339..>3170)
                     /locus_tag="AC16VR_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009600.1"
                     /translation="GFKSGGFDNGAIYSTLNPDPLADITFEPETVEGFEAGIKAALFD
                     GTVNVELDAYYYEYEELQLNFFNSSVFAYRTLNAGAAETTGSELQVKWAPPGVDGLTL
                     LASVAYNDASYKDFIAPCSGGQSFSGGCNIGTPPVPFGPPTLMDQNLKGEPRNLAPEW
                     AGIVGFDFTRGIGSGLQIGLTGNMQWKSDYSLSEFIPSARQDGFVWIDAALRFGAESG
                     RWQLAFIGKNLTDEYVLVSARDTAQTGGNTGTDFAYPADRIGTALRPRTFEMELSVRF
                     "
BASE COUNT          542 a         1047 c         1037 g          544 t
ORIGIN      
        1 cggcgacctc cgcaccgaac aggctcgagc ctaagatcca taccggcacc ttggaccccg
       61 ccccgggcac cgcatgaacg cgctgaccct cgaccggatc gcccagatag agcatgagct
      121 ccatgaggtc ctggggaaac ccgtccacgt cgctgttgag cgtgcgtctg agcgcgtgag
      181 ccgtgatctg gtccgtgccc ggtgctcggc cgagaccgag gtcgatccgg cccgggtaga
      241 gtgcttccag ggtgccgaac tgctcggcga tcaccagcgg cgcgtggttc ggcagcatga
      301 tgccgccggc tcccacgcgg atcgtggacg tgccttcggc cacgaaaccg atggcgacgg
      361 cggtggccga gctggcaatg ccgggcatgt tgtggtgctc ggccagccag aaccgctcga
      421 atccccaggc ttccccatga cgcgccaggt cgcgcgcgtt gtgcagcgcc tggcgtgcgg
      481 atccgccaat ggtgatgggg gccaggtcca gaatactgaa tcggggcatg ctcgctcccg
      541 cggtctaaaa cagctccagc ataaacccga tgagcgatcc gatcacgaat atgtagatgg
      601 ggggcatgct gagataaagc ccgggcccac gcttctcggc cgcctcgtag taggcaaacg
      661 cataccagag ccggccgacg ggccaaagaa ccccgatccc cgccgcccag atggggtcca
      721 cggcgtagga gaacagccac agcccgggca gaaacagcac caggtgctcc atggtgttct
      781 gatgcacgcg aaccctgcgg acgtactcct cagggccctc atgagacggc gccggtatgt
      841 tgtatttgcc ccgggccatg ccggccttga acagggtgaa gtagtaaacg agcagcgcca
      901 ggctgctcag aatgcccggg taaacgtagt cacccatgtc gctctcttcc gctcaccgaa
      961 gccggattgg cagccgtttc ccgatcgccg ctcaaggccg ctcgccggcg atgtactcgt
     1021 cgatcacccc atggaaccgt gcgatgcgcg actcctggtc tgccagattg gcgccccggt
     1081 aaccgcgcga gcgcagcccg agctgttgcc cggtggtaat gccgatgtcc tgatcggcga
     1141 tgggacccat ggattccttc ccgtactcaa cttcctttat ctcggcgtcg cccgtgaatt
     1201 cgacccggcc gtctgagttg cggaacaccc cgtgctcgat ggcggctggt gccacgtagg
     1261 tccagtgatc gaaacggcac tgctgcggat cggtgggatg aggccgcatc cgctgcagca
     1321 gcacagagtc ggcaccgaag gtcaccgagc agttggggaa gatgttgtag tggtgcggat
     1381 cggtgagctg ctcgtcgcgc aggcggtcgt agtgagcata gccccgcgga gcgccgagat
     1441 cgcgcatctg ctgctggatc gccgcccggg tatcatgctc ccggccggcg aagtcgtcgg
     1501 ggtcgagctc ccacatggcc atctgggaga gcagcggttc cacgagcttt ggcccctggg
     1561 cctggcgatg ggacggcatg cccgcctgca tgatcatgcg gttgtggcct tcgttggcca
     1621 tgtcgaagcg cgtccacggc acgccttcct cgatgttcgc cgtgagctgc gggtgcacgg
     1681 tcagcaggtg gtaggactcg cagaagttgt cctgtatgac cttccagttg cagggtaccc
     1741 aagcggagat ggccagggac cggcgccagt cctcgggttg ccacgtctgc cagtcttccc
     1801 acacgggacc gaggtattcg cgcagcgaga cgcagtcggg gtccatgttg acccacaccc
     1861 agccggcgaa ggtctcgcag cgcacctcca ccaggctcag ccgctcgcag ggattcccgg
     1921 agctgaaatc ctcagggtcc tgcacgacca gcagcgcgcc gtcacggccg taggtccacg
     1981 agtggtaagg gcaggagatg cgctccacgg aaccggcggc attgaagatc agccgcgagc
     2041 cccggtgctg gcagacgttg tagaacgcgc ggatggaccc gtcgtcctgg cgcaccatga
     2101 tgaaggactc gggcccgacg tcctccacct ggtagtcgcc cgggttcggc atctcccggg
     2161 cccggccaag cagcagccag accctgctcc acatcccgtc ccactccgca gcggcaaagc
     2221 cgcggtccgt gtacctgctg ccttcgatgt gatgcgcggg cacgtcgggc tgcgcccagg
     2281 cgggcgcagg ctccgatcgc cagtcgcggg cgttggtaac caatagctcc gctccgcgct
     2341 agaagcgcac gctcagttcc atctcgaacg ttctgggccg cagcgccgtg ccgatccggt
     2401 ccgcggggta ggcgaagtcc gtgcccgtgt tgccaccggt ctgtgccgta tcgcgagcgc
     2461 tgaccagaac gtactcgtca gtcaggttct tgccgatgaa ggccagctgc cagcggccgc
     2521 tctcagcgcc gaagcgcagc gccgcgtcga tccagacgaa gccgtcctga cgcgccgacg
     2581 ggatgaactc gctcagggag tagtccgact tccactgcat gtttccggtg aggccgatct
     2641 gcagcccgct gccgatgccc cgggtgaagt cgaagcccac gatgcccgcc cactcgggcg
     2701 ccaggttgcg cggctcgcct ttaaggttct gatccatcag cgtcggagga ccaaagggca
     2761 cgggcggggt gccgatgttg cagccgccgc tgaaggactg cccgccgctg cacggggcga
     2821 tgaagtcctt gtagctcgca tcgttgtagg ccaccgaggc caggagcgtc aggccatcga
     2881 cgcccggcgg cgcccatttc acctgcagct cgctgcccgt ggtctccgcc gcgccggcgt
     2941 tcagcgtacg gtaagcgaac acgctggagt tgaagaagtt cagctgcagc tcttcgtact
     3001 cgtagtagta ggcgtcgagc tccacgttca cggtaccgtc gaacagggct gccttgatgc
     3061 cggcttcgaa gccctccacc gtctccggct cgaaggtaat gtcggccagc gggtccgggt
     3121 tcagagtgct gtagatggcg ccgttgtcga agccgccgga cttgaagccc
//
LOCUS       DBNHQR010000010         2322 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1204470, whole genome shotgun sequence.
ACCESSION   DBNHQR010000010 DBNHQR010000000
VERSION     DBNHQR010000010.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1204470"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..783)
                     /gene="uvrY"
                     /locus_tag="AC16VR_00225"
     CDS             complement(121..783)
                     /gene="uvrY"
                     /locus_tag="AC16VR_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003284948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HIR6009601.1"
                     /translation="MIRVMVVDDHKLFRLGLRRMLADVKGIHVIGEADCGEAAVRLAR
                     AERPNIVLMDILMPGIGGIEATRRIARLEMDIKVIAVTGCHENPFPMQMLRAGACGYL
                     SKGVDRDELVAAIRKVFLGRRYLSADIAQEVAASAFDQTRESPFEQLSNREMQIMMMV
                     VNCHKVNDISEDLHLSPKTVNSYRYRIFEKLNVSSDVELALMAVRHGMVIPQLNGMAR
                     AV"
     gene            complement(923..2101)
                     /locus_tag="AC16VR_00230"
     CDS             complement(923..2101)
                     /locus_tag="AC16VR_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008367295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HIR6009602.1"
                     /translation="MPALEPFVWDDPLLLEDQLTEDERMVRDTAREYAQRELMPRVLK
                     ANREEHFDREIMNEMGALGLLGATLPAKDGCSAVNYVSYGLMAREVERVDSGYRSAMS
                     VQSSLVMHPIYAYGNEDQRERYLPKLASGEWVGCFGLTEPDHGSDPGGMVTRAEPVSG
                     GYRLNGAKMWITNSPIADLAVVWAKLDGVIRGFVVERAFDGFSTPKIEGKMSLRASVT
                     GEIVLDDVVVPEENLLPNVKGLAGPFGCLNRARYGIAWGSMGAAEFCWHAARTYTLDR
                     EQFGRPLAANQLIQKKLADMQTEIALGLQGCLRMGRLLDEDRLAVENISMLKRNNCGK
                     ALEVARMARDMHGGNGISDEYHVIRHVMNLETVNTYEGTHDIHALILGRAQTGLQAFS
                     "
     gene            complement(2184..>2322)
                     /locus_tag="AC16VR_00235"
     CDS             complement(2184..>2322)
                     /locus_tag="AC16VR_00235"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6009603.1"
                     /translation="GGDHAGLGLHIVHRLVEEVKARIDVRTSTDSGTTFTVFLPLSQP
                     V"
BASE COUNT          430 a          755 c          698 g          439 t
ORIGIN      
        1 agcttggcgc gcacagaccc cgtggcgccc acgggcaggc agcggtaacc gggatgggct
       61 tgctgtacct gatcgagatg agcggccaac cgagggcaga tgcggcaact ttccaggcgg
      121 tcataccgca cgggccatcc cgttcagctg gggtatcacc atgccgtggc gtaccgccat
      181 cagggcgagc tccacgtcgc tggagacgtt gagcttctcg aagattcggt accgatagct
      241 gttcaccgtc ttcggcgaaa ggtgcaggtc ctcggagatg tcgttcacct tgtgacagtt
      301 gaccaccatc atcatgatct gcatttcacg attggaaagt tgctcgaacg ggctctcccg
      361 ggtctggtcg aacgcgcttg ccgccacctc ctgagcaatg tcggcgctca ggtagcgcct
      421 gccgagaaag acctttcgga tggccgcgac cagctcgtcg cggtcgacgc ccttgctgag
      481 atacccgcag gctccggccc ggagcatctg catggggaaa gggttttcgt ggcacccggt
      541 cacggcgatc accttgatgt ccatctccag acgcgcgatc cgccgggtgg cttcgatgcc
      601 gccgataccg ggcataagga tgtccatgag aacgatgttc ggtctctccg cgcgggcgag
      661 ccgcaccgcc gcctccccgc agtccgcttc gccgattacg tgtatacctt tgacgtccgc
      721 cagcatgcgc cgcagcccca gacgaaacag cttgtgatcg tccacaacca taaccctgat
      781 catcttgccc ccaacaaagt gtccagcatc tgaagcagtg acgataggcg ggcgccaggc
      841 ggctaacaac ggcgcggggt gagaatctgc cgggttttca cgaaatttcc attttccgat
      901 gtcgcgctga tatcagatgc gatcaggaaa acgcctgcag accggtctgg gcgcgcccca
      961 gtatcagcgc gtggatgtcg tgggtcccct cgtaggtgtt gaccgtctcc agattcatga
     1021 catgccggat cacgtggtac tcgtcggaga tcccgttgcc gccgtgcatg tcccgcgcca
     1081 tgcgcgcgac ctccagcgct ttgccgcagt tgttgcgctt gagcatggag atgttctcga
     1141 cggcaagacg atcctcgtcc agcaggcgac ccatcctgag gcagccctgc aggccgaggg
     1201 cgatctccgt ctgcatgtcc gcgagcttct tctgaatcag ctggttggcg gcgagcgggc
     1261 gcccgaactg ctcccgatcg agcgtgtagg tgcgtgccgc atgccagcag aattcggctg
     1321 cccccatgga tccccaggcg atgccgtagc gcgcgcggtt caagcaaccg aagggaccgg
     1381 ccaggccttt gacgttgggc agcagattct cttcgggaac aacgacgtcg tcgagcacga
     1441 tctccccggt gaccgacgcc cgcaggctca tctttccctc tatcttgggg gtgctgaaac
     1501 cgtcgaacgc gcgctcaacc acaaacccgc ggatcacgcc gtcgagcttt gcccagacca
     1561 ccgccagatc ggctattggc gagttggtga tccacatctt cgcaccgttc agacggtagc
     1621 cgcccgacac cggctcggcc ctggtcacca tgccgccggg gtccgatccg tggtcgggct
     1681 ccgtcaggcc gaaacagccg acccactcac cgctggccag cttcggcagg tagcgctcac
     1741 gctgatcctc gttgccgtaa gcgtatatcg gatgcatgac cagcgaggat tgcacgctca
     1801 tggccgaccg gtaccccgaa tccacgcgct ccacttcccg ggccatcagg ccgtaagaga
     1861 cgtagttgac ggccgaacag ccatccttcg ccggcagcgt ggcacccagc agaccaagcg
     1921 cccccatctc gttcatgatt tcccggtcga agtgttcttc gcgattggcc ttgagaacgc
     1981 gcggcatgag ctcgcgctgc gcgtattcac gcgccgtatc gcggaccatg cgctcgtctt
     2041 cggtcagctg atcttccagg agcaacggat cgtcccagac gaacggttcg agagcaggca
     2101 tgggctcatc ccagtttgcg gaaagtcagg cccaagggta ggagcgatac ggccgctagg
     2161 caactggcgc ctggccaggc atatcagacc ggctgggaga gcggaaggaa gacggtgaaa
     2221 gtggttccgc tgtccgtact ggtacgaacg tcgattcgag ctttcacctc ctccaccagc
     2281 cggtgaacga tgtgaagacc gagacccgca tgatcgcctc cc
//
LOCUS       DBNHQR010000011         6038 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_64155, whole genome shotgun sequence.
ACCESSION   DBNHQR010000011 DBNHQR010000000
VERSION     DBNHQR010000011.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6038)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6038)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6038
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_64155"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="AC16VR_00240"
     CDS             complement(<1..746)
                     /locus_tag="AC16VR_00240"
                     /inference="COORDINATES: protein motif:HMM:NF012879.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR6009604.1"
                     /translation="MRSVFRFMCFMLFVSAGPASLARGAVDVDQLAIPHETFVLENGL
                     TVIVHEDHSVPIVAVNLWYHVGSRNESRGRTGFAHLFEHFFFNGSEHYPHDFRRAMDD
                     VGANNRNGTTNPDRTNFFEDVPVSALERTLYLEADRMGFLAANITQETLDQERGIVQN
                     EKRQGENQPYGKVWNRIVESIYPYSHPYSWPIIGSMADLEAATLDDVKAWYAKYYGPS
                     NCVLSLAGDITVEQARELVGKYFAGIPPGPP"
     gene            complement(977..1498)
                     /locus_tag="AC16VR_00245"
     CDS             complement(977..1498)
                     /locus_tag="AC16VR_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009605.1"
                     /translation="MKGKVLFFSLVFYAVVGMWLWGEYQQRPRVLDGLGQVMLGMTPA
                     QVVGVLGRPTEEFAPAEDEQRRLEYSYRSDGTSDYLVVFWPDEEGEPKADIICREGGS
                     VFGLAVGQSSELDIIDKLGEPSHRSFGPDGRAKAISYESFKVAFEIEGGWTREICVSQ
                     TGSISYALESITV"
     gene            1730..3556
                     /locus_tag="AC16VR_00250"
     CDS             1730..3556
                     /locus_tag="AC16VR_00250"
                     /inference="COORDINATES: protein motif:HMM:NF025056.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HIR6009606.1"
                     /translation="MADIFVSYASEDLDRVKPLITALEERRFAVWWDREIHPGTSWDE
                     AIERELESATWIVAIWTANSIRSRWVRTESMAALERDALIPILLDDVTLPVAFRTTQV
                     VDLRQWRPEGSDATFERLVSHLQGKPHPPAEVEAHRQRSPPNRRGPLLAALALTALAA
                     IALVWMVVTEPVRAPSDAGLAVTTFAGQPRIAVLPFDHGSDPEDRPFANGLTDEVIKG
                     LQQYRTIPVISRHASFALRDKGLSVQEIASQLGARYLVTGAVSRRGDRLRVSAGLTDA
                     DGHQLWKESYEVDFDPESVFALQDQIAEQIAGVSYPQILEGEIDRVARRDVTSLDAWG
                     HAVNAVEIINAMNVDRLDEGLLHAERALELDPNLPLAYWARAEIKVYQLVERGLTGQS
                     ADAMEDEILEDFQEALDISPFDGALCGCLGFVRVMRGEIEQAEAVFDTVLPVNPSNAI
                     LRTNYATLLTHQGKLEEARREAELAIRLDPLSRFGSMSWSTLSLVAAMDNDLEQAIQL
                     ARRALQVEGTETYGQVQLPMLLYLKDRIPEAQDAFADLLKHHPRVNPRNRLTYGWFRP
                     IEPLIREHVAQASGTLRKERSLADLLEWIFLDLGWEPPTPMP"
     gene            3736..4854
                     /locus_tag="AC16VR_00255"
     CDS             3736..4854
                     /locus_tag="AC16VR_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009607.1"
                     /translation="MQIRTQAAAVLIVLLAGGCAVQQPVQPTGIANITRDQIGRMAVR
                     SPDRPKVALAANVDTKGAAARKTALSAGATWLGGSFEAAGQAGEGGLLIAAFGLVTAP
                     IVAAGGAVYGAAAADSKEEITSGNEVLVRTLDFAPAQMQRALENELTTSAPMDWEFVD
                     PDASNAELAARGFDSVLDMDMDSIASHVSANDFHVYFDTLNRLTLTSLVSGQSLAIRK
                     YWRELPPRAVSDWAGDGGSVLTNALHEQFADIAREMADDLFLYPAIRVHGVEPVSRRR
                     FGAGTISGRRPMFVWSALDGGNGTPAQAVEYELAIAADRQANPVSFRTHKMRYVPPDS
                     LEACRTYSWKVRAHYLSFGEPTTSQWSPEYRFKTPCRK"
     gene            complement(4908..>6038)
                     /locus_tag="AC16VR_00260"
     CDS             complement(4908..>6038)
                     /locus_tag="AC16VR_00260"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6009608.1"
                     /translation="IGALVVPITEEFGWQRSQFQMAILFSSGLGALTAPVVGWLIDRY
                     GARRIALPGILGLSVGFVLATFMDGRLWMLYLSYGAMALLGAGTIPVTWTRAITTNFF
                     RQRGLALGLTLSGTGLCAVIVPQYAVWMVEAYGWRAAYLGLAAMPLLIAGPVVYFGFR
                     PREGIGGEALETGEAQWGMTLGEAFRTTKYWVLLISIFVVYMAVSGIGPNLIPALVDD
                     GLTPAQAATALSTFGGAIIAGRIIVGYLVDRFWAPGVAALSLSLPVAGCLMLVGAPGF
                     ETAVAAALLIGIAAGAELDLMSFLAAKYFGLRHYAKIYAVLYATLAVCSGTAPMLFAR
                     VYDVTASYDVSFWISTVLFALGAVLVLAMGAYPRMGDSRELP"
BASE COUNT         1186 a         1980 c         1915 g          957 t
ORIGIN      
        1 ggaggacccg gagggatccc tgcgaagtac ttgccaacga gttccctggc ctgctcgacg
       61 gtgatgtcgc ccgccaggga cagcacgcag ttggacggac cgtagtactt ggcgtaccag
      121 gctttgacat cgtcgagcgt ggccgcttcg aggtcggcca tgctgccgat gatgggccag
      181 ctgtaggggt gcgaataggg gtagatcgac tccacaatgc ggttccagac ctttccatag
      241 ggctggtttt cgccctgacg cttttcgttc tgaacgatgc cgcgctcctg atccagagtc
      301 tcctgagtga tgttggcggc gagaaagccc atgcggtcgg cttccagata gagcgtgcgc
      361 tcgagcgcgg agacaggcac gtcctcgaag aagttcgtgc ggtccgggtt ggtcgtgccg
      421 ttgcggttgt tggcgccgac gtcgtccatg gcgcgacgga aatcgtgcgg gtagtgctcg
      481 ctgccgttga agaagaagtg ctcgaagagg tgcgcaaagc ccgtccggcc ccggctttcg
      541 ttacgggatc ccacgtggta ccagaggttc acggcgacga tgggcacgct gtggtcctcg
      601 tgcacgatca cggtgagacc gttttcgagc acgaacgtct cgtggggtat ggccagctgg
      661 tcaacgtcaa cggcgccccg ggcaaggctt gccggtcctg ctgagacaaa caacataaaa
      721 cacatgaacc ggaagacact gcgcatcagt cacacctcgt tgtcgccatg gctcgcgaga
      781 acacggcgat caccggccat ccggcctgcg ccacttccgc cgggctgaag cgcgagtatc
      841 gcatccggat cactggaagc aaccgcccgg gggaaacggc tgatcgaaaa gggaagtgaa
      901 attcgccagc agcgtgtcca ggtcgatgct gccgctgtgc gactccctgg cgtccagcca
      961 gtgcccgctg ctgaatctag acggtgatcg attccagcgc gtagctgatg gagcccgttt
     1021 ggctgacgca gatctcccgc gtccatccgc cctcgatctc gaaggcaacc ttgaagctct
     1081 cgtaagagat ggcctttgcg cggccgtccg ggccgaagct gcgatgggac ggctcaccga
     1141 gcttgtcgat gatgtcgagc tcggaagact gacccacggc cagcccgaag acgctgccgc
     1201 cttcccgaca gatgatgtca gccttcggct cgccctcttc gtccggccag aaaaccacga
     1261 ggtaatcgga cgtaccatca ctgcggtagg agtactcgag gcgccgctgt tcgtcctccg
     1321 cgggggcaaa ctcctcggtc ggccggccca gaacacccac cacctgcgcc ggcgtcatgc
     1381 caagcatcac ctgccccaac ccgtcgagga cgcgaggtct ttgctggtac tcgccccaca
     1441 gccacatacc gacgacggcg tagaacacca gcgagaagaa caggaccttt cctttcattg
     1501 cgggattcgt ttccttgagc agccaatccg tggcacaggc caccgcgcct gaagattctg
     1561 cacgttagat cgagaaaacg ccttgcgctg aggcgcggga agtttgtgac ccagcgcaca
     1621 atcccagtgg acgcgggagc gaaaaggccg tccgggaaga cgagtgcaca ttctgctagg
     1681 cttccattca cttgatcatg ccggccggac ctgggcggaa gcgggatgca tggcggacat
     1741 cttcgtctcc tacgcaagtg aggacctcga tcgcgtaaaa ccgctgataa ccgcgttgga
     1801 ggaacgcaga ttcgccgtgt ggtgggaccg cgagatccac cccggcacca gctgggacga
     1861 ggcgatcgaa cgcgagctcg agtcggcaac gtggatcgtg gcgatctgga ccgccaactc
     1921 catacggtcc cggtgggtgc gcacggagtc gatggccgct ctcgagcgcg acgcgctcat
     1981 accgattctt ctcgacgacg ttacccttcc cgtggctttc cgtacgactc aggtagtgga
     2041 cctgcggcaa tggcggccgg aaggatcaga cgcgacgttc gaaaggctgg taagtcacct
     2101 ccaggggaaa ccgcacccgc ccgccgaagt cgaggcgcac cgacaacgat cgcctccaaa
     2161 ccggcgcggc ccgctcctcg cagcactggc cctaaccgcc ctcgccgcca ttgccctggt
     2221 ctggatggtc gtgaccgaac cggttcgagc accgtccgac gcgggcctcg ccgtgaccac
     2281 gtttgccggg caaccaagga tagccgtgtt gccgttcgac cacggcagcg accccgagga
     2341 tcgcccgttc gccaacggac tgaccgacga ggtcatcaaa ggtctgcagc aatatcgcac
     2401 catcccggtg atatcgcggc atgccagctt tgcgttgcgg gacaaggggc tgtccgtcca
     2461 ggagatcgcg agccagcttg gcgcgcgcta cctggtgacc ggagccgtca gccgccgggg
     2521 cgacaggctc agagtcagcg ctggcctgac cgatgctgac gggcaccagc tctggaagga
     2581 gtcctacgag gtggacttcg accccgagtc ggttttcgcg ctccaggacc agatcgccga
     2641 gcagatcgcc ggcgtgagct acccgcagat tctcgaggga gaaatagacc gggtagcgag
     2701 gcgcgacgtc accagcctgg atgcctgggg gcacgccgtg aacgcggtag agatcataaa
     2761 cgccatgaac gtcgatcggc tcgacgaagg actactccat gccgagcggg ctctcgagct
     2821 ggatccgaac cttcctctcg cgtactgggc aagggcggaa ataaaggtgt atcagctcgt
     2881 cgagcgcggc ctcaccgggc agtcggcgga cgccatggag gacgagatcc tcgaggactt
     2941 ccaggaagcc ctggacatca gcccgttcga cggcgcgctg tgcggctgcc tggggttcgt
     3001 gcgcgtcatg cgcggcgaga tcgaacaggc cgaggcggtg ttcgataccg ttctgcccgt
     3061 caacccgagc aacgccatcc tgcgcacgaa ctacgcgacc ctgctgactc atcagggcaa
     3121 gctggaagag gcccggcgcg aggccgagct ggccatccga ctggaccccc tcagccgttt
     3181 cggttccatg tcctggtcta ccctgagcct ggtagcggcc atggacaacg acctcgagca
     3241 ggcgatccag ctggcccggc gggcgcttca ggtggagggc accgaaacat acggtcaggt
     3301 gcagctgccg atgctgctct acctgaaaga ccgcattcct gaggctcagg atgccttcgc
     3361 cgatctgttg aagcatcacc ccagggtgaa tccccgcaac cggctcacct atggttggtt
     3421 ccggcccatc gagcccctga tccgcgagca cgtggcccag gcgtccggca cgctgcgaaa
     3481 ggagcggtcg ttggccgacc tgctggaatg gatctttctg gatctgggct gggaaccgcc
     3541 aaccccgatg ccctgacggc tcgacgtggc gcttcgacgt ccacgtgttt acgggcgcaa
     3601 ggccaaattt aaatataaat aaatcagata gttaccggcg cctaagaaaa ccacaacaag
     3661 cttcagccgc acatcaggat ctgcgcgcgc ggcgatgcga ggctgcctgc cacgtacacg
     3721 cagggagaca gccagatgca gatccgaacc caagccgccg ccgtcctcat cgtgcttctg
     3781 gccggaggct gcgcggtcca gcaacccgtt cagcccacgg ggatcgcgaa catcacccgg
     3841 gaccagatcg gccgcatggc cgtgcgcagc ccggatcgtc ccaaggtggc gctggcggcg
     3901 aacgtggaca cgaagggcgc ggcagcacgc aaaacggcgc tctcggcggg ggcaacctgg
     3961 ctgggcggat ccttcgaagc ggcaggtcaa gccggtgagg gaggattgct gatcgccgcc
     4021 ttcgggctcg tcaccgcccc catcgtcgcc gccggagggg ccgtctacgg ggccgcagcc
     4081 gctgattcca aggaagagat cacttccgga aacgaggtgc tcgttcggac gctggacttc
     4141 gcgcccgcgc agatgcagcg ggcactggag aacgagttga cgacgtcggc gcccatggat
     4201 tgggaattcg tcgaccccga tgccagcaac gcggagctcg ccgcgcgcgg cttcgactcc
     4261 gtgctggaca tggacatgga cagcatcgcc agccacgtga gcgcaaacga cttccacgtc
     4321 tatttcgaca ccctgaatcg tctgaccctg accagcctcg tgagcggcca gtcgctcgcc
     4381 atcagaaagt actggcggga gcttccgccc cgggcggttt ccgattgggc cggtgacggc
     4441 ggcagcgtcc tgaccaatgc cctgcacgag cagtttgccg acatcgccag agagatggcg
     4501 gacgacctct ttctctaccc cgccatccgg gtccacggcg tcgagcccgt ctcgcgccga
     4561 cgattcggcg ctggcacgat ctccgggcgg cggcccatgt tcgtctggtc ggctctggac
     4621 ggcgggaacg gcacacccgc ccaagctgtc gagtacgagc tcgccatcgc ggccgaccga
     4681 caggccaatc cggtgagctt tcgaacccac aaaatgcgct acgtgccccc ggactccctg
     4741 gaggcctgtc gaacctacag ctggaaggtt cgcgcccact acctgagctt cggcgagccg
     4801 accacgagcc aatggagccc ggagtatcgg ttcaagacac cgtgcagaaa gtagatgtcg
     4861 ccaaccctcg ccctgacacc acgctacgcg ccctgcatcg gagcggatca gggcagttcc
     4921 cgtgaatccc ccatccgcgg atacgcgccc atggctagga cgagcacggc gcccagcgcg
     4981 aacagaaccg tggagatcca gaagctcacg tcgtagctgg ccgtcacgtc gtacacccgg
     5041 gcaaacagca tgggagccgt gcccgagcag acggccaggg tggcatatag caccgcgtaa
     5101 atcttggcgt agtgacgcag accgaagtat ttcgccgcca gaaacgacat cagatcaagc
     5161 tctgcacccg ccgcgatccc gatcagcagc gcggcggcca cggcggtctc gaagccgggg
     5221 gcgcccacca gcatcaggca gccggccacg ggaaggctga gggacagggc cgccactccc
     5281 ggcgcccaga accggtccac gaggtaaccc acgatgatcc gcccggcaat gatcgcgccg
     5341 ccgaacgtgc tgagcgccgt ggccgcctgg gcaggcgtca gcccgtcgtc caccagcgcc
     5401 gggatgaggt tggggccgat gccggaaacc gccatgtaaa ccacgaagat ggagatcagc
     5461 agcacccagt acttggtagt ccggaaagct tctcccaggg tcatacccca ctgagcctcg
     5521 ccggtctcca gggcctctcc gccaatcccc tcccgggggc gaaagccgaa gtacaccacg
     5581 ggcccggcga tcagcagcgg catggccgcg agcccgaggt acgcggcgcg ccagccgtaa
     5641 gcttccacca tccagacggc atactgagga acgatcaccg cgcacagccc cgtgccgctg
     5701 agcgtaagcc ccagggcaag gccccgttgt cgaaagaagt tggtggtaat ggcacgggtc
     5761 caggtgaccg gaatcgtccc cgcgccgagc agggccatgg cgccataaga cagatagagc
     5821 atccacaggc gcccgtccat gaacgtcgcc agaacgaagc cgaccgacag cccgaggatc
     5881 cccggcaacg cgatgcgccg agccccgtag cgatcgatga gccagccaac cacgggcgcg
     5941 gtgagcgcgc ccaggccgga gctgaagagg atggccatct gaaactggga gcgctgccag
     6001 ccgaactcct ccgtaatggg caccaccagg gcgccgat
//
LOCUS       DBNHQR010000012         2878 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1331422, whole genome shotgun sequence.
ACCESSION   DBNHQR010000012 DBNHQR010000000
VERSION     DBNHQR010000012.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1331422"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="AC16VR_00265"
     CDS             complement(<1..306)
                     /locus_tag="AC16VR_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008172631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HIR6009609.1"
                     /translation="MAIQEGDKLPEATMHIIQDGRPTPVSTGDLFAGKKVVVFAVPGA
                     FTPTCSQAHLPGYVVNADAIKAKGVDSIVCLSVNDVFVMDAWGKDQNTGESVAMVADG
                     "
     gene            complement(398..1057)
                     /locus_tag="AC16VR_00270"
     CDS             complement(398..1057)
                     /locus_tag="AC16VR_00270"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6009610.1"
                     /translation="MECDERLLELISENLARFEASDADVDDAHHAAVAITVTDVGPGA
                     DLPGLSGTDGWQSQAGLLLTRRSAQLRKHAGQWAFPGGRLDPGETPAETALREMHEEV
                     RVDLGPDRILGRLDDFVTRSGFVMTPFVVWGGAGLQASPNPDEVQSIHRIPLTEFLRE
                     DAPVLEDADSSDRPVLKMPVGTEHIAAPTAALIYQFREVCLLGRHTRVAHYEQPAFAW
                     R"
     gene            1166..2050
                     /locus_tag="AC16VR_00275"
     CDS             1166..2050
                     /locus_tag="AC16VR_00275"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR6009611.1"
                     /translation="MSHDSERQALVAALAAVLLWSTVATGFKLGLAVMAPLQLLLAGT
                     TVSAIFFAAVTTARRSWSVTGRQLGEAAAFGIVNPALYYLVLFEAYDRLPAQLAQPLN
                     YTWAITLAVLAVPILKQRLSARTLAGILVSYLGVLVLLSQGRFDRLPAVDWTGVMLAL
                     GSTLLWAGYWLMNARSRSDSVNLMALSFLIATPLVAIACVLGPGLPTLDWNVAWFGAW
                     VGLVEMGVTFLLWQRAMGLTSHAARIGQLIFLSPFISLMLISTVLGETIHWTSVAGLV
                     VIVGGVLLTRSAAPGRAR"
     gene            2118..2714
                     /locus_tag="AC16VR_00280"
     CDS             2118..2714
                     /locus_tag="AC16VR_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6009612.1"
                     /translation="MIRTTTAVVTALLALAAAPNLTASESDTIARGQYLVTLLGCGRC
                     HTEGYLTGDKATGPYLGGSRVGIAYTAYSMDDTHPGVVFAGNLTPDDATGLGTWSEEE
                     IVQAMTVGVAKSGHERLTVMPWTNYGALKEKDVRAIARYLKSLSPVRWPIPEAIPEGD
                     PIDEPYVRFGVYKFYPHGPVEAINLPVPTNEPSPEGVQ"
BASE COUNT          439 a          988 c          934 g          517 t
ORIGIN      
        1 gccgtccgcc accatggcga cgctctctcc ggtattctgg tccttgcccc aggcatccat
       61 gacaaagaca tcattgacgg acaggcacac gatggagtcg acgcctttgg ctttgatggc
      121 gtccgcattg accacgtagc ccggcaggtg agcctgggag cacgtgggcg tgaacgcgcc
      181 cggtacggca aagaccacca ccttcttgcc ggcgaaaaga tcgccggtgc tgaccggcgt
      241 cgggcgcccg tcctggatga tgtgcatggt ggcttctggg agcttgtcgc cttcttgaat
      301 cgccatggcc ctttaatgtc cttcttctgt tgagcgggtc cgaaagtttg gggaaagacg
      361 cttcgatggt caacgaacgc tgatcgatcc gggcggatca gcgccaggca aaggccggct
      421 gctcgtagtg ggcgacccga gtgtgccggc cgagcaggca gacctcccgg aactggtaga
      481 tcagcgccgc ggtgggcgct gcgatgtgct cggttcccac cggcatcttc agcacgggac
      541 ggtcgctgct gtcggcatcc tccagcaccg gcgcgtcctc ccgcagaaac tcggtgagcg
      601 gtatgcggtg gatggactgc acctcgtcag gattcggcga tgcctgaaga cccgccccgc
      661 cccagacgac gaagggcgtc atcacgaacc cggagcgcgt cacgaagtcg tccagacgac
      721 cgaggatccg gtcggggcca agatcgaccc gcacctcctc gtgcatctcc cgcagcgcgg
      781 tctccgccgg cgtctcgccg gggtcgagcc gcccgccggg aaacgcccac tggccggcat
      841 gctttctgag ttgcgccgag cggcgggtga gcagcaggcc tgcctgggat tgccatccat
      901 cggtgcctga aagacccggc agatcggcac cggggccgac gtcggtgacg gtaatggcca
      961 ccgcggcgtg gtgggcgtcg tccacgtccg catcgctggc ttcgaagcgg gccaggttct
     1021 cgctgatcag ttccagcagg cgctcatcgc attccatcga agcagccgtc aatttgggcg
     1081 gtgtgtggcg agcccccttc ggggcccgtt cgcgaatcat gcgccctggt gtacgctgac
     1141 gcaatttcac gccccggcga ccccattgag ccacgactcg gaaaggcagg cactcgtcgc
     1201 cgcgctcgcg gccgtgctgc tgtggtccac ggtcgccacc ggcttcaagc tggggcttgc
     1261 ggtgatggcg ccgctgcagc tgctgctggc cggaaccacg gtctccgcca tcttcttcgc
     1321 cgccgtcacc accgcgcgcc gcagctggtc ggtgaccggc cgccagctgg gggaagcggc
     1381 cgcgttcggc atcgtcaatc ccgcccttta ctacctggtt ctgttcgagg cctacgaccg
     1441 gctgccggcc caactggccc aacccctgaa ctacacctgg gccatcaccc tggcggtgct
     1501 ggccgtcccc atcctgaagc agcgtctctc ggcgcgcacc ctggccggta tcctcgtgag
     1561 ctatctgggg gtgctcgtcc tgctctccca agggcgcttc gaccgcctgc ccgccgtgga
     1621 ctggaccggc gtgatgctgg ccctgggcag cacgctgctg tgggccggct actggctgat
     1681 gaacgcccgt tcccggagcg attccgttaa cctgatggct ttgagctttc tgatcgcgac
     1741 tcccctggta gccatcgcct gcgtgctggg ccccggcctg ccgaccctgg actggaacgt
     1801 cgcgtggttc ggcgcgtggg tgggtctggt ggagatgggg gtgacttttc ttctgtggca
     1861 gcgggccatg gggctgacct cccacgcggc tcgcatcggc cagctcatct ttctctcgcc
     1921 cttcatttcc ctgatgctca tctcgacagt gctgggtgaa accatccact ggacgtccgt
     1981 cgccggcctc gtcgtcatcg tcggcggcgt gctcctgacc cgatctgcgg cgcccgggcg
     2041 ggctaggtga ttcccgcggt atcgcgacgc cgccaagtcg ggcaccatcg gcccactgtc
     2101 acaagtggat gcaacccgtg atcaggacga ctaccgcggt ggtgaccgcc ctcctcgcac
     2161 tggccgccgc cccgaacctc acggcctcgg agtcggacac cattgccaga ggccagtacc
     2221 tggtcaccct gctgggctgc gggcggtgtc ataccgaggg ctacctcacc ggcgataagg
     2281 ccacgggtcc ctatctcggc ggttcccgcg tgggcatcgc gtacacggcg tacagcatgg
     2341 acgacaccca tccaggggtg gtcttcgccg gcaacctgac ccccgacgac gcaaccggtc
     2401 tgggaacctg gagcgaagag gagatcgtcc aggccatgac cgtgggcgta gccaagagcg
     2461 gtcacgagcg cctgaccgtg atgccgtgga cgaactacgg ggcgctcaag gaaaaggacg
     2521 tccgagccat cgcccgctat ctgaagagcc tctcccccgt gcgctggccg atccccgagg
     2581 ccattcccga gggagatccg atcgacgagc cctacgtacg cttcggcgtc tacaagttct
     2641 acccccacgg cccggtggag gccatcaacc tgccggttcc caccaacgag ccgtcgcccg
     2701 aaggggtgca atgaccgcaa caagccgcgg gctttcgtgc ggctccaggg ccgacggggt
     2761 tgcagattcc gggcgcgccg cgatctaggc gccgcggaac tccggcttgc gcttggccat
     2821 gaaggcgttg atgccctcct ggccgtcggc tgtcacggat agctcggcga tcgctcgg
//
LOCUS       DBNHQR010000013         2724 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_317979, whole genome shotgun sequence.
ACCESSION   DBNHQR010000013 DBNHQR010000000
VERSION     DBNHQR010000013.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_317979"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="AC16VR_00285"
     CDS             <1..216
                     /locus_tag="AC16VR_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009613.1"
                     /translation="TWDRFLEGVERYRACISDFVRSNYAASDAHRDAANAATLDWNAF
                     VRDSLNVPEDFPWEPEDSDDQAEPDNP"
     gene            297..1757
                     /locus_tag="AC16VR_00290"
     CDS             297..1757
                     /locus_tag="AC16VR_00290"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WS/DGAT/MGAT family O-acyltransferase"
                     /protein_id="HIR6009614.1"
                     /translation="MQQLTGLDASFLYLETPKTPMHIGGVMIYDQRTVKGGKLRFKDI
                     LDHFESRLHLARTFRQKLVHVPFNLDHPYWIEDRDFDLEFHVRHIRLPEPGDWRQLCI
                     QVARLHSRPLDLTKPLWEFTIIEGLDDIEGLPRGSFAVVTKIHHACIDGVSGVDMVEA
                     IHDVEPYVDRSPGPVTPWKGEQEPSAIELMVRAQVHNMTQPYRLAEVLARTVPALGRL
                     GQRATGGDAERASLAPPRTRFNHVVSAHRVIEGRRFDLAEIRAIKDTVDGATVNDVVL
                     AVCGGALRRYLLAKHELPGVPMIAMAPISVRSSDEKGAMGNRISAMTISLGTHIEDPI
                     GRLSAIHEAAVASKGMSNAVGAKLLTDYSQFIPSTTAAMAARLYVQLGLSERVNIPFN
                     CVVTNVPGPQFPLYSAGAKLLAQYGLGPIFDGMGLIFPVFSYNGKLFLSVTSCREMMP
                     DPEFFSECMQAAFDELKAAALVIPEAADASLAGGTP"
     gene            complement(1778..2452)
                     /locus_tag="AC16VR_00295"
     CDS             complement(1778..2452)
                     /locus_tag="AC16VR_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009615.1"
                     /translation="MGQTDSTSRSDVIDQLVASYLKIPLKIYWRETRGLPFPDTFIDA
                     RLEFAGMTTSWLRLDSVIWRAGEVRFVPGFPARLYVRSPSVEFVVGQAELDRWQSRFS
                     LPYRLELTESGITVHTELAGFPLAEFETGLTVVDGWFVLEPKRATVLGVPGYVSSLFR
                     GYLPVPPLSGEIRLAGIEHAAGILRVRFDIDDFDEEVTPGMLSRLQKRLFSFNSPLGP
                     TRRDDA"
     gene            2507..>2724
                     /locus_tag="AC16VR_00300"
     CDS             2507..>2724
                     /locus_tag="AC16VR_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009616.1"
                     /translation="MSGQTNSVGGEKVPLVIGVTGHRDLLAEELPVIRRRVQDLFAEL
                     QRQFPDLPILVMTPLAEGADRLVAEVAR"
BASE COUNT          489 a          860 c          897 g          478 t
ORIGIN      
        1 acctgggacc gcttcctgga aggcgtcgag cggtatcgcg cctgcatcag cgacttcgtg
       61 cgcagcaact acgcggcgtc cgatgcgcat cgggacgccg cgaacgcggc caccctggac
      121 tggaacgcgt tcgtccgcga ttccctcaac gttcctgagg atttcccctg ggagcccgag
      181 gactccgacg accaggcgga gccggacaat ccttagcggc tgccgccgcc gagttggtct
      241 gcggagcgct aaaggcggat aatgccccct gcccagcgac caccagcggc ggacagatgc
      301 agcaactcac cggactagac gcctcttttc tctacctcga aactccgaag acgccgatgc
      361 acatcggcgg cgtcatgatc tacgaccagc gcacggtcaa gggtggcaag ctgcgcttca
      421 aggacatcct cgatcacttc gagagccgtc tgcatctggc caggacgttt cgtcagaagc
      481 tcgtgcacgt gcctttcaac ctggatcatc cctactggat cgaggatcgc gatttcgatc
      541 tggaatttca cgtgcgtcac atccgtctcc cggagcccgg tgactggcgt cagctctgca
      601 ttcaggtggc gcggctgcat tccaggcctc tggacctcac caagcccctg tgggagttca
      661 ccatcatcga ggggctggat gacattgaag ggcttccccg gggaagtttc gcggtggtga
      721 ccaagatcca ccacgcctgc atcgatggcg tgtccggcgt ggacatggtg gaggccatcc
      781 acgacgtcga gccctacgtc gaccggtcgc ccggaccggt cacgccgtgg aagggcgagc
      841 aggagcccag tgccatcgag ctcatggtcc gtgcccaggt ccacaacatg acgcagccct
      901 accgccttgc ggaggtgctg gcccgtacgg tccccgccct gggcaggctc ggccagcggg
      961 cgacgggggg cgacgcggaa cgggcttccc tcgcgccgcc gcgaaccagg ttcaaccacg
     1021 tggtatcggc tcatcgtgtg atcgaggggc ggcgattcga cctggcggag ataagggcca
     1081 tcaaggacac cgtggatggg gcgaccgtca acgatgtggt gctggccgtc tgcggcggcg
     1141 ctctgcggcg ctacctgctg gcaaagcacg agctgcccgg cgtgcccatg atcgccatgg
     1201 cccccatttc tgttcgcagc tccgacgaga aaggcgccat gggcaaccgg atctccgcca
     1261 tgacgatctc gctgggcact cacatagagg accccatcgg gcggcttagc gccattcacg
     1321 aagcggcggt ggcctccaag gggatgagca acgcggtggg cgcgaagctg ttgaccgact
     1381 attcgcagtt catcccgtcg acgacggcag ccatggcggc ccgcctgtac gttcagctgg
     1441 ggttgagcga gcgggtcaac attccgttca actgcgtcgt tacaaacgtg ccggggcctc
     1501 agtttcccct gtacagcgcg ggagcgaagc tgctggcgca gtatgggctg ggcccgatat
     1561 tcgacggcat ggggctgata tttcccgtgt tcagctacaa cgggaagctc tttctgtccg
     1621 tcacctcctg ccgggagatg atgcccgatc cggagttttt ctcggaatgc atgcaggcgg
     1681 ctttcgacga gctcaaggcg gctgcgctgg tcattccgga agccgctgac gcctcgctgg
     1741 ccggtggaac gccctgatgc tcgggagccg ttcgcggcta cgcgtcgtcc ctgcgcgtcg
     1801 gccccagtgg cgagttgaag gagaacagcc gtttctggag ccgggacagc atgcccgggg
     1861 tgacctcctc gtcgaagtcg tcgatgtcga atcgcacgcg cagaatgccg gcggcgtgct
     1921 cgatgccggc tagacgtatc tcgccggaca ggggcgggac cggcagatag ccgcggaaaa
     1981 gggatgacac gtagccgggc acaccgagca cggtggctcg tttgggttcc agaacgaacc
     2041 acccgtccac gaccgtgagg cccgtctcga actcggctag aggaaatccg gccagctccg
     2101 tatgcacggt gatcccggac tccgtgagct cgagccgata cggcagcgag aaccgcgact
     2161 gccagcggtc gagctctgcc tggcccacga cgaactccac gctgggcgac cgcacgtaga
     2221 gccgagccgg aaaccccggg acgaatcgca cttcaccggc acgccagatc acgctgtcca
     2281 ggcgcagcca ggaagtcgtc atgcccgcga attcgagtcg tgcgtcgatg aaggtgtcgg
     2341 ggaaagggag cccgcgggtc tcgcgccagt agatcttcag cggaatctta aggtaggaag
     2401 cgaccagctg gtcgattacg tctgaacggc tcgtcgaatc ggtctggccc atgagcggag
     2461 tttacccagg ctttgcctgg gaacaaatac gggttaagtt tccgccatgt ccggccagac
     2521 caacagcgtt ggtggggaaa aggtgccgct cgtcatcggc gtgacggggc atcgggatct
     2581 tctcgctgag gagctgccgg tgatccggcg gcgggtccag gatctgtttg ccgagctgca
     2641 gcggcagttc cccgatctgc cgatcctggt catgacgcct ctagccgagg gggcggaccg
     2701 gctcgttgcc gaggtggcca ggga
//
LOCUS       DBNHQR010000014         2804 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_318076, whole genome shotgun sequence.
ACCESSION   DBNHQR010000014 DBNHQR010000000
VERSION     DBNHQR010000014.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_318076"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1613)
                     /gene="glgB"
                     /locus_tag="AC16VR_00305"
     CDS             complement(<1..1613)
                     /gene="glgB"
                     /locus_tag="AC16VR_00305"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003113646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HIR6009617.1"
                     /translation="MIRADSADHPQLGAEITQALVRGDHSDPFAVLGPQRIAGRWAIT
                     ALVPGAETVRAIAPDGTVLAELHPVGASELFTGWVAGNVHPDAYRLQAERGTARWEQE
                     DAYRFGPVLGEHDEHFLSEGTHLRLWEVLGAHPVHHEGASGVRFAVWAPHASRVSVVG
                     DFNGWDGRRHAMRARGVTGVWEIFVPGIGAGERYKYEIRAPDGSVLPLKADPVGLGAE
                     HPPATASIVRDLPRRRWGDGDWMQRRGELQRTDAPVSIYEVHLGSWKRRAEEGQRPLS
                     YLEHAVELVPYVRDMGFTHIELMPVSEHPFDGSWGYQPVGLYAPTIRYGTPEEFRDFV
                     EACHGAGLGLILDWVPGHFPTDTHGLGQFDGTALYEHADPREGYHQDWHTLIYNYGRR
                     EVANYLVANALYWLREHHVDGLRVDAVASMLYRDYSRAEGKWVPNRYGGRENLEAVAF
                     LKRVNEVVYGDDPSVMTAAEESTSWPGVTRPTSAGGLGFGYKWNMGWMNDTLDYMRLD
                     PIHRRHHHHQMTFGLLYAFSENFILPLSH"
     gene            complement(1977..2774)
                     /locus_tag="AC16VR_00310"
     CDS             complement(1977..2774)
                     /locus_tag="AC16VR_00310"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HIR6009618.1"
                     /translation="MLVDRSRHPVKLTAAGSQMLEAGLQALARIEHERSQILDALSLP
                     DKYVVTFGAQHSIGWRFFPAWLQALEEAYGPILSRLRADDLPNCMRDLKNGDVDFVIA
                     YTGPESGRAASAPFQPFDAVSIGQDRLIPVCKPNSDGTPLFSFENQNIEMPWLRFGDS
                     APIGKHLEPLLNAKNLRQRLKTVYENSMAGALRIRARDGTGVAWLPESLVAPDLAVGM
                     LVRTGEADWAVDLQIRLLRNKAFSNRLTRSIWSYLEVRQSVGLLPRE"
BASE COUNT          494 a          945 c          885 g          480 t
ORIGIN      
        1 tcatggctga gcggcaggat gaagttctcc gagaacgcgt aaagcagccc gaatgtcatc
       61 tggtggtgat ggtggcggcg gtggatcggg tcgaggcgca tgtagtcgag cgtgtcgttc
      121 atccagccca tgttccactt gtagccgaat ccgagcccgc cggcgctggt cggcctggtc
      181 acgcccggcc aggaggtcga ctcttcggcc gcagtcatga ccgaggggtc gtccccgtag
      241 acgacctcgt tcacacgctt caggaaggcg accgcctcca ggttctcgcg cccgccatac
      301 cggttcggca cccacttccc ctcggcacgg gagtagtcac ggtagagcat cgaagcgacc
      361 gcatcgacgc gcagcccgtc cacatggtgt tcgcgcagcc aatagagcgc gttggccacc
      421 aggtagttcg ccacctcgcg ccgcccataa ttgtagatca gggtatgcca gtcctggtgg
      481 tacccctcgc gcggatcggc atgctcgtag agcgccgtgc cgtcgaactg gccgagcccg
      541 tgcgtgtcgg tggggaaatg gcctggcacc cagtcgagaa taaggccgag ccccgcgccg
      601 tggcaggctt ccacgaagtc gcgaaattcc tcgggcgtgc catagcggat ggtcggcgcg
      661 tagagaccaa cgggctgata gccccaggac ccgtcgaagg ggtgctccga gaccggcatc
      721 agctcgatat gcgtaaaccc catgtcgcgc acatagggca ccaactcgac ggcatgctcc
      781 aggtaggaga gcgggcgctg cccctcctcc gcccggcgct tccacgagcc cagatgcacc
      841 tcgtagatcg agaccggcgc atcggtgcgc tgaagctcgc cgcgccgttg catccagtct
      901 ccgtctcccc accggcgccg gggcaggtcg cgcacgatcg acgcagtggc ggggggatgc
      961 tcggcgccaa gacccaccgg atccgccttc agcggcagca cgctgccgtc gggcgcccgg
     1021 atctcgtact tatagcgctc gcccgcgccg atgccgggaa cgaagatctc ccagacaccc
     1081 gtcacgccgc gagctcgcat ggcatggcgc cgtccgtccc agccgttgaa atcgccaacc
     1141 accgagacgc gcgaggcgtg gggtgcccag acggcgaaac ggacacccga ggcgccctcg
     1201 tggtggacgg gatgcgcccc gagcacctcc cacagcctca gatgagtgcc ttccgagagg
     1261 aaatgctcgt catgttcgcc aagcaccggc ccgaagcggt aggcatcctc ctgttcccag
     1321 cgggcggtac cccgttcggc ctgcaggcgg taggcatcgg gatgcacgtt gccggcaacc
     1381 caaccggtga agagctcgct ggctccaacc ggatggagct cggccagcac cgtgccgtcc
     1441 ggtgcgatgg cgcgcacggt ctcggcgccg gggacaagag cggtgatcgc ccagcggccg
     1501 gcgatccgct gcggacccag caccgcgaag ggatcggagt ggtcgccacg taccagcgcc
     1561 tgggtgattt cggcaccgag ttgcggatgg tctgcgctgt ctgctcggat caattccggg
     1621 ccctttcccg atgcgcgtca ttgcttatac ggtcagccga cgcgacagtg caatctttcg
     1681 cccctcacgc ggtgtccgct cccggcacgt cgcaggttcc ttgcatattg cggggtcgcg
     1741 aatgtcacga ctctgtagct cgcttgtgcc gaggtttcgc gccaagccgc catgagcggc
     1801 gacgcgacac gtccggcacc aacgttgcgg caagcccctc cggtacgccg gcaattacac
     1861 ttcacctgcg gggctgtcaa ttattaaatc actctgattt ttttatacgt tcagccacgg
     1921 accgccaaac cgatgcgagc cacagccttt acaaaagcgc ctggcgcgct ccgaggtcat
     1981 tctcgcggca gcaaaccgac ggattgcctg acctccaaat acgaccatat cgatctggtc
     2041 agacggttcg agaacgcctt gttgcgcagc aatctgatct gcagatcgac ggcccaatcc
     2101 gcctctccgg tgcggaccag cattccgacg gcgaggtcgg gcgccaccag gctttccggc
     2161 agccatgcga cgcccgtgcc atcgcgcgcc ctgatgcgca acgcgcccgc catcgagttc
     2221 tcgtagacgg tcttcagccg ctggcgaagg ttctttgcgt tcagcagcgg ctccagatgc
     2281 ttgccgatcg gcgcggaatc gccgaaacgc agccaaggca tctcgatgtt ctgattctcg
     2341 aagctgaaca gcggcgttcc atcggaattg ggcttgcaga ccgggatcag cctgtcctgg
     2401 ccaatggata cggcatcgaa tggctggaac ggtgcagacg ccgcgcggcc gctctcgggt
     2461 ccggtatagg caatgacgaa gtccacgtcg ccgttcttga ggtcacgcat gcagttgggc
     2521 aggtcgtcgg ctcgcaaccg ggacaggatc gggccatagg cctcctccag cgcctgcagc
     2581 caggcaggga agaagcgcca cccgatcgag tgctgtgcgc cgaaggtcac cacgtatttg
     2641 tccggcaggg acagcgcatc caggatctgg ctgcgctcgt gctcgatccg cgccaacgcc
     2701 tgcagccctg cctcgagcat ctgcgatccc gcagcggtca gcttgaccgg gtgacgcgag
     2761 cggtcgacca gcatggcccc gacccaggct tccagcgtct tgat
//
LOCUS       DBNHQR010000015         3004 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1521814, whole genome shotgun sequence.
ACCESSION   DBNHQR010000015 DBNHQR010000000
VERSION     DBNHQR010000015.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1521814"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="AC16VR_00315"
     CDS             <1..606
                     /locus_tag="AC16VR_00315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HIR6009619.1"
                     /translation="GWSLLSSFIYAAPAAVVFELWRLGGTAVYMDLAAMPLWYLPVSV
                     GVYLFLHDTYFYWTHRLMHQKRVFPIMHRVHHESRPPTPWASFSFHPYESVLGAIFLP
                     ALALVIPIHIGAVLFILTVMTLAAVLNHSGYEIFPRWWLRGWPGHHLITAAHHDLHHK
                     HFSCNYGLYFRFWDKLMRTDIMESEYDFLAPSAEVPASPHR"
     gene            complement(542..1882)
                     /locus_tag="AC16VR_00320"
     CDS             complement(542..1882)
                     /locus_tag="AC16VR_00320"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6009620.1"
                     /translation="MSGPVLTSSFGLRMSAFTALYLAQGVPIGLFSIALPAWLVEQGA
                     DAADIAALVAVTGLPWAFKLIAGPFMDRFSFPAMGRRRPWVMGAQAGFTVSMVSLALV
                     SGPSDGLWPIMVIGFVINTFAAVQDVAVDGMAIDVLPISERGRANAFMAFGQAAGFSG
                     FGALDGYLLSRFGLGVTAIVSAAAIGAVLVFVAVTREREGERLLPWTAGMATAREIAP
                     ARSMLEILRDILRVLLLPMSLLLIAVEFLARAGSGVGLAIMPVLAVDELGYASEVYAY
                     WLGLMGGLSAFVGIFCGPAIDRLGARKVLVVGLLGGSLVTVAYALAAGARGTAGFDLT
                     MLAAAQIFGQVFFVSMIAMFMGICWARVAATQFAIYMSLANLARSAGAGAFALVATDL
                     SVFDALYLVAALSVAAVLLLLRFDPAAHAARLALIDEERLAPRQTERESRTPTP"
     gene            2012..>3004
                     /locus_tag="AC16VR_00325"
     CDS             2012..>3004
                     /locus_tag="AC16VR_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009621.1"
                     /translation="MTAPKRTPASKTDAAGSIGVSWRLASDIRTLAVLASLVVSAWLI
                     ATGDIVNNDGMLYLSAAERIAQGDWEAARGLYGWLFYPWLIAVISGIAPLGLEASAHL
                     VDAAMAAVMVYAFLSIVRELGGDRKVELIAAFVIVFHPYLNDSRAEILRDHGYWALYL
                     LATFYFLKCHRNPSWPNALSWACLMALATLFRIEGSVILVALPFLLLLKRDELPARRL
                     RAFGRAYTLNALVLAAVAVWAAVEPDFTDQAGRLLDPIRKLNQLFASVGEGFQIKAQR
                     LHDAVLTGYTDQYAVPALLAVVFLIMTDNVVRTLSPLYVVLPAFRRFRARLTFPH"
BASE COUNT          555 a         1020 c          891 g          538 t
ORIGIN      
        1 ggctggtcac tgctgtcctc gttcatctac gctgcaccag cggccgtggt cttcgagctg
       61 tggcgcctcg gcggcaccgc cgtctacatg gacctggccg ccatgccgct ttggtatctg
      121 cccgtgagcg tgggggtcta tctctttctg cacgatacct atttctactg gacccatcgt
      181 ctcatgcacc aaaagcgggt ttttccgatc atgcaccggg tccatcacga gtccaggccg
      241 cccacgccct gggcgtcatt ctcgtttcac ccctacgagt ccgttctcgg cgccatcttt
      301 ctgcccgcgc tcgctctggt gattcccatt cacatcggtg ccgttctctt catattgacc
      361 gtgatgacac tcgctgccgt gctgaatcat tcgggatacg agatctttcc ccgatggtgg
      421 ctgcgtggct ggcccgggca ccacctgatc acggctgccc accatgacct tcatcacaag
      481 cacttcagct gcaactatgg tctctacttc cggttctggg acaagctgat gagaacggac
      541 atcatggagt cggagtacga ctttctcgct ccgtctgccg aggtgccagc ctctcctcat
      601 cgatgagcgc cagccgggcg gcgtgagccg caggatcgaa tcgcaggagc agcaaaaccg
      661 cggcgacgct cagcgctgcg accaggtaga gcgcatcgaa tacgctgaga tccgtcgcga
      721 cgagcgcaaa cgcgcctgcg cccgccgacc tggccaggtt cgccagcgac atgtagatgg
      781 caaactgggt agcggcgact cttgcccagc agatgcccat gaacatggcg atcatcgaca
      841 cgaagaagac ctgaccgaat atctgggcag cggccagcat ggtgagatcg aatccggctg
      901 tacccctcgc ccccgccgcc agcgcataag caaccgttac cagcgagccg ccgagcaggc
      961 ccaccacgag caccttccgg gcgcccaggc gatcgatggc gggcccgcag aatatgccga
     1021 cgaaggcgct cagcccgccc atcaatccaa gccagtaggc gtaaacttcc gatgcgtaac
     1081 ccagctcgtc tacggccagc acgggcatga tggccaaccc gactcccgaa cccgcgcgcg
     1141 caagaaactc cacggcgatg agaagcaggc tcatgggcag caggagcacc cggagaatgt
     1201 ccctgagaat ttccagcatc gatcgggccg gggcgatctc ccgggcagtc gccatgcccg
     1261 ccgtccaggg cagcagacgc tccccttccc gctcccgtgt caccgcgacg aagactaaga
     1321 ccgcgccgat ggctgcagcc gatacgatgg cggtgacgcc cagaccaaaa cggctgagca
     1381 ggtagccgtc cagcgcaccg aatcccgaga agcccgctgc ctggccgaac gccatgaaag
     1441 cgttcgcccg gccccgctcg ctgatcggca gcacgtcgat ggccatgccg tcgacggcca
     1501 cgtcctgaac ggcggcgaac gtgttgatca cgaagccgat caccatgatc ggccagaggc
     1561 catccgaagg gccggacacc agggccaggg agaccatgga tacggtaaag ccggcctggg
     1621 cacccatgac ccagggtcga cgccgaccca tggcggggaa actgaagcgg tccatgaagg
     1681 gcccggcgat cagcttgaag gcccagggaa gcccggtgac ggcaacgagc gcagcgatgt
     1741 ccgcagcatc cgcgccctgc tccacgagcc atgccggaag agcgatcgag aacagaccga
     1801 tgggtacgcc ctgcgccagg tacagggcgg tgaatgccga catgcgcagc ccgaaggacg
     1861 aggtgagaac cggcccgctc acagggacac gctcccacga tcgtgacgac gcttattttc
     1921 ctgacccgcg tcttccaata tgctgattcc ctggtaagac aagatgagca acggctcgaa
     1981 gcgccgcgct gccgagctta acccatagaa cgtgacggct cccaaacgga cacccgcctc
     2041 taaaacggac gcggccggat ctatcggagt ttcctggcga ctggccagcg acatccggac
     2101 gctggccgtt ctcgcaagcc tcgtggtgtc agcctggctg atcgccaccg gcgacatcgt
     2161 caacaacgac ggcatgcttt acttgagcgc cgcggagcgg atcgcccagg gtgactggga
     2221 ggcggcacgt gggctgtatg gctggctgtt ctacccttgg ctcatcgccg tcatcagcgg
     2281 gatcgcgcca ttgggccttg aagcgtcggc ccatctcgtc gacgcggcca tggcagcagt
     2341 catggtgtat gccttcctca gcatcgtccg ggaactcggc ggcgatcgca aagtcgagct
     2401 catcgccgct ttcgtgatcg tcttccaccc ctatctgaac gactcgcgcg cggagattct
     2461 gcgggatcac gggtactggg ccctgtacct gttggcaacc ttctacttcc tgaaatgcca
     2521 tcgcaatcct tcctggccga acgccctcag ctgggcttgc ctgatggccc tggccacgct
     2581 cttccgcatc gagggttccg tgatcctcgt ggctttgccg tttcttcttc tgctcaagcg
     2641 cgacgagcta cccgcgcggc gcctgagggc attcgggcgg gcgtacaccc tgaacgctct
     2701 cgtcctggca gccgtggccg tctgggccgc ggtcgagccc gactttaccg accaggccgg
     2761 tcggctgctg gatccaattc gcaagctgaa ccagctgttt gcgtccgtcg gcgagggctt
     2821 ccagatcaag gcccagcgtc ttcacgacgc cgtgctgacc gggtacacgg accagtacgc
     2881 tgtgcccgct ctgctcgcgg tggtgttcct gatcatgacg gacaacgtcg ttcgtacgct
     2941 gtcgccgctt tacgtcgtcc tgccggcatt ccggcgcttc agggcccgcc tgacgttccc
     3001 gcac
//
LOCUS       DBNHQR010000016         3514 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_825105, whole genome shotgun sequence.
ACCESSION   DBNHQR010000016 DBNHQR010000000
VERSION     DBNHQR010000016.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_825105"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="AC16VR_00330"
     CDS             complement(<1..252)
                     /locus_tag="AC16VR_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HIR6009622.1"
                     /translation="MIARAALVLTTALLVVLAPDQAKAEAPDDLERGKAIASDRNAGN
                     CLSCHMMGDGELPGNSGPPLLQMRLRFPDRAVLRAQIWDA"
     gene            complement(249..1187)
                     /locus_tag="AC16VR_00335"
     CDS             complement(249..1187)
                     /locus_tag="AC16VR_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6009623.1"
                     /translation="MYRFLSAALVVLAAGCGAPQEAAEPSKPTYDDSRVGHFGYGKPA
                     SAEEIAGWDIDIRPDGLGLPQGSGSVEDGEWLYEAQCAECHGSFGEGVGRFPVLSGGK
                     GSLTDPRPSKTVGSYWPYTSTLYDYIRRTMPFTQPESLTADETYAVTAYVLYLNDLVE
                     DDFVLTHENLASVRLPNEGNFVPDPRPDVSNTRCMTDCRDPAAISVLSEVPTYVPASM
                     TMEEIAEAEPAHPGAATYDQYCSICHDVGVAGAPRIGDAAAWSSRLERGMAALEMNAI
                     NGIASEAGVMPAKGGFAHLADDEVNAAVAYMVEGSQ"
     gene            complement(1171..2484)
                     /gene="soxC"
                     /locus_tag="AC16VR_00340"
     CDS             complement(1171..2484)
                     /gene="soxC"
                     /locus_tag="AC16VR_00340"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017134966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HIR6009624.1"
                     /translation="MRQRQLDQIVKSVAESGDKLPREERRRFLRQGLSLAGATAAASF
                     AGGALADSGNLPPNVPSWTRRLGPGVLTNPYGQPSPFESHVQRRTVDWLTADRIAAIS
                     FTPLGDLNGIITPSGVVFERYHAGAPAIDPNQHRLMIHGMVKRPIILTMDDLMRFPSV
                     SMIRFLECPANGGMEWRGAQMDRLQFSHGMLACCEWTGVLLSTLLEEVGLEADASWIL
                     AEGADGAHMSRSIPMEKALDDALIVYAQNGEALRPEQGYPVRLLNPGWEGNTSIKWLR
                     RLEVGDKPWHHREETSKYTDLMADGTAREFTFVQECNSVVTHPCPERPLKDKGKYFIE
                     GLAWSGRGKIRHVDVSVDGGVTWREARFTSQVLPRALTRFGLEWDWDGSPALIQSRAV
                     DETGYVQPTYGQLKAVRGTNSIYHKNAIHTWRLEPNGTVSNVQIS"
     gene            2621..2956
                     /locus_tag="AC16VR_00345"
     CDS             2621..2956
                     /locus_tag="AC16VR_00345"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR6009625.1"
                     /translation="MELEVMQENAGRAADLMKLLGHPHRLMILCQLKEGECSVGELSE
                     KIGINQSPLSQHLARMRHEGVVSSRREAQTVYYSLAGPEVAAVISLLYELYCQPANEE
                     MQAPTSAAR"
     gene            complement(3014..>3514)
                     /locus_tag="AC16VR_00350"
     CDS             complement(3014..>3514)
                     /locus_tag="AC16VR_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009626.1"
                     /translation="RMLHRRWNDHLNVADLCVVWGANAAQLDLNEFEFRNGQFTCFFK
                     TRDQAAWRAFEKAGLSNNHLLTEDPYLRHMIERVRIGDQIRIRGWLASYGNEYGFSRG
                     TSTTRTDTGNGACETIYVRDFAILASMENGWRTVFDGSALLAVTLGLLWTVGVMGGRF
                     EHRSEY"
BASE COUNT          583 a         1134 c         1147 g          650 t
ORIGIN      
        1 ggcgtcccag atctgcgctc gcagcacggc ccggtccggg aatctcaggc gcatctgcag
       61 caggggcggg ccgctgttgc ccggcagctc gccgtctccc atcatgtgac aggacaggca
      121 gttgccggca ttgcgatcag atgcgatggc cttgccccgc tccaggtcgt cgggggcctc
      181 ggccttggcc tggtccggcg ccaaaacgac cagcagggcg gttgtcagga ccagcgcggc
      241 tcgtgcaatc attgagaacc ctccaccata taggccaccg ctgcattgac ctcgtcgtcc
      301 gccaggtgcg cgaagccgcc ttttgccggc atgacgccag cctccgatgc gattccgttg
      361 atggcgttca tttccagggc cgccatgccg cgctcgaggc gcgatgacca ggcagcggcg
      421 tcgccgatgc ggggtgcgcc ggcgacaccg acatcgtgac atatgctgca gtactgatcg
      481 tacgtcgccg cgcccggatg ggccggttcg gcctcggcga tctcctccat ggtcatggag
      541 gccggcacgt aggttggaac ttcggagagc acgctgatgg ccgcggggtc gcggcaatcg
      601 gtcatgcagc gcgtgttgct cacgtccggc cgggggtccg gcacgaagtt gccctcgttg
      661 ggaagacgca cggaggccag attttcgtgg gtgagcacga agtcgtcttc gaccagatca
      721 ttcaggtaca ggacgtaggc ggtcaccgcg tacgtctcgt cggccgtcag ggactccggc
      781 tgcgtgaagg gcatggtgcg gcggatgtag tcgtacaggg tgctggtgta gggccagtag
      841 ctgccaacgg ttttgctggg ccgcgggtct gtcagcgagc cctttccgcc agacagcacg
      901 gggaagcgcc cgaccccttc gccgaaggat ccgtggcact cggcgcactg cgcctcgtag
      961 agccactcgc cgtcctcgac ggatccgctc ccctggggaa gtcccaggcc gtccggtctg
     1021 atgtcgatgt cccaccccgc gatttcctcg gcgctcgccg gcttgccgta gccgaagtgg
     1081 ccgacgcggc tgtcgtcgta ggtcggcttg gatggttcgg ccgcctcctg gggggcaccg
     1141 cagccagcgg cgaggacgac gagcgcggca ctaagaaatc tgtacattgc tcaccgtccc
     1201 gttgggttcc agccgccagg tgtgaatggc gttcttgtga tagatggagt tggtgccgcg
     1261 cacggccttg agctgcccgt aggtgggctg aacatagccg gtctcgtcga cggcgcggct
     1321 ctggatcagt gcgggcgagc cgtcccagtc ccactccagc ccgaatcgcg tcagcgccct
     1381 tggcagcacc tggctggtga agcgcgcttc ccgccaggtc acgcctccat ccaccgagac
     1441 gtccacgtgc ctgatcttgc cgcggccgga ccaggcgagc ccttcgatga aatactttcc
     1501 tttgtccttg aggggccttt cggggcaggg atgggtcacg acggagttgc actcctggac
     1561 gaaggtgaac tcgcgtgcgg tgccgtctgc catcaggtcg gtgtacttgg aggtctcttc
     1621 gcggtgatgc cacggtttgt cgccgacctc gagccgtcga agccacttga tcgaggtgtt
     1681 gccctcccat cccggattca gcaggcgcac cggatatccc tgctccggcc gcagggcctc
     1741 gccgttctgg gcgtacacga tgagcgcatc gtccagcgcc ttctccatgg ggatgctgcg
     1801 gctcatgtgg gcgccgtccg cgccttcggc caggatccag ctggcgtcgg cctccagccc
     1861 gacctcctcg agcagggtgg agagcagcac gcccgtccac tcgcagcagg cgagcatgcc
     1921 gtggctgaac tgcagccggt ccatctgggc gcctcgccac tccatgcctc cgttggcggg
     1981 acattcgaga aaccggatca tgctcaccga cgggaagcgc atcagatcat ccatggtcag
     2041 aatgatcggg cgcttgacca tgccgtggat catcaaccgg tgctggttcg ggtcgatggc
     2101 cggcgcgccg gcgtgatagc gctcgaacac cacgccgctc ggcgtgatga tgccgttgag
     2161 atcgcccaga ggcgtgaagc tgatggcggc gattcggtcc gccgtcagcc agtccacggt
     2221 gcgccgctgg acgtgggatt cgaaggggga gggttggccg taggggttgg tcagaacgcc
     2281 cggacccagc cgccgggtcc atgacgggac gtttggagga aggttgcccg aatccgcaag
     2341 cgccccgccg gcgaagctcg cagccgccgt ggctccggcg aggctcaaac cctgccgcag
     2401 gaagcgtctt cgctcctccc gtggcagctt gtccccggac tcggcaacgg acttcacgat
     2461 ctgatccagc tgacgttgac gcacgctccc tccgcgtata ttttccgtcg cgcgatgcga
     2521 agagttagta tattataaat ttcttaagta aaggcaagcc gaaatatcgg ctggatttgg
     2581 agggtcgaaa aacggcggcc cgccatcgga gtattcgttg atggaactcg aagtcatgca
     2641 ggaaaacgcc ggccgggcgg ccgacctgat gaagctgctc gggcacccgc accggctcat
     2701 gattctctgc cagctcaagg aaggcgaatg ctccgtgggc gagctcagcg agaagatcgg
     2761 catcaatcag tcgccgctgt cccagcatct tgcccgcatg cgccacgagg gcgtggtgag
     2821 cagcaggcgc gaggcgcaga ccgtctacta ctcgctcgcc ggcccggaag tcgcagcggt
     2881 gatctcgctg ctctacgagc tctactgcca accggcgaac gaggagatgc aggcgccgac
     2941 ttcggcggcc cgctgagccc cctcgagacg ctttcaggcg tcagccgtcc ttatcacagt
     3001 cggttctacc cggtcagtat tcggatcggt gctcgaagcg cccgcccatg acgcccaccg
     3061 tccacagcag ccccagggtg acggccagca gagcactgcc atcgaaaacg gtgcgccagc
     3121 cgttttccat cgacgccaga atcgcgaaat cccgaacgta gatggtctcg cacgcgccgt
     3181 tgccggtgtc cgtacgcgtc gtgctcgttc cccgtgagaa accgtactcg ttgccgtagc
     3241 tcgcaagcca tccccggatg cggatctggt cgccgatcct tacccgttcg atcatgtgcc
     3301 gaaggtaggg gtcctcggtt agcaggtggt tgttggacag cccagccttt tcaaacgcgc
     3361 gccaggcggc ctgatccctg gttttgaaaa agcacgtgaa ctggccgttc ctgaactcga
     3421 actcgttcag gtcgagctgc gcggcgttcg caccccaaac cacgcacaga tcggcgacgt
     3481 tgagatgatc gttccagcga cggtggagca tgcg
//
LOCUS       DBNHQR010000017         4290 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_825108, whole genome shotgun sequence.
ACCESSION   DBNHQR010000017 DBNHQR010000000
VERSION     DBNHQR010000017.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4290)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4290
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_825108"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="AC16VR_00355"
     CDS             complement(<1..291)
                     /locus_tag="AC16VR_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009627.1"
                     /translation="MNQKEIDELRQLMEYEAARSEPPEGFPSLPDLPGGRYTDQRFYD
                     LEQQHIWRKSWLLAGHIDEIPEPGCFRLWETAGQPVVLVHADSGAVNAFYNTC"
     gene            complement(341..1105)
                     /locus_tag="AC16VR_00360"
     CDS             complement(341..1105)
                     /locus_tag="AC16VR_00360"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003288959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6009628.1"
                     /translation="MSGRFDGKVVMITGATSGLGKHAAVAFAREGARVVASGRRQAEG
                     EETLAEIEAAGGTAVFVQADVSVSEDVARIVAAARDRFGGLDWAYNVAGVPGDAFAKA
                     AEYSEDAWDETMEINVKGVFLCVKHAIPAMLERGGGAIVNMSSIAGIGGSPGGVAYVA
                     SKHAIIGLTKAAALDYAEQNIRINALAPGVIYTDMLAWGITQTPGLEADLISQHPIGR
                     LGQLEEVTNAAMWLCSDEAGFVTGTTLTVDGGHLAR"
     gene            1280..1735
                     /locus_tag="AC16VR_00365"
     CDS             1280..1735
                     /locus_tag="AC16VR_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009629.1"
                     /translation="MPKVVKASVWVVGLLVVAAGGYYAYLQLVGNPRVMTEIRAEPDG
                     ERARRVMLIDLPDGRTLPVNYLREDDMVYMGVDGLWWRQFRQGDVPVELLIRGEDLQG
                     HARAVLDDPDRTRRVFARLRPTAPDWLPGRMGGVLVEIRLQAATLDEED"
     gene            1785..2486
                     /locus_tag="AC16VR_00370"
     CDS             1785..2486
                     /locus_tag="AC16VR_00370"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR6009630.1"
                     /translation="MKEFLSARAVEFVSINVLEDREARERLARAGIRTLPVVSRDDAF
                     VPGMDLARVAEFLGLDYQGGNILPADVLVSRYHRVLDAAVRYVGQIPPARLHDTLPHR
                     DRSYLALGNHLVQIAVDFLSVTRGAALTGRLAESVPETELEPPALMAAAAQAKRGLAD
                     WLAAADSAELASPVVTYFGSQTLHQVLERAVWHSAQHGRQLMMVLQRLGISPDGPLTD
                     EDFAGLPMPEEVWDG"
     gene            2579..3826
                     /locus_tag="AC16VR_00375"
     CDS             2579..3826
                     /locus_tag="AC16VR_00375"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6009631.1"
                     /translation="MQLPTDEEWARACAADGEFGLAARHWTGGLTLAVGEAELSLRLT
                     DGAVSPGRAESAEGLLTYAGPADVWEQVLAAQPPRFHNDIMANLTVGGGLNRQVDPVV
                     HAQYYPAVMRAVELLRPAEPPFMPMRDEARPPGAFDAPVGRYVHLELEGHDHRIYFEE
                     AGQGIPLLMQHTAGCHGSQWRHLFEIPEITSRFRLIAYDLPYHGKSVPPVSREWWAER
                     YELKGDFLRSVPVKLAEALGLDRPVFMGCSVGGLLALDLALRHAEAFRAVISVEGALN
                     IEGDLGELEELWHPQVSNEYKARLMDGIMAPASPKAYRKETSFVYACGWPPVFLGDLY
                     YYINDFDIRQAAGQIDTSRVGVHILSGEYDYSGTSELGRQAHELIPGSTWTEMKGVGH
                     FPMTENPVRFVEYLEPILASISA"
     gene            3890..>4290
                     /locus_tag="AC16VR_00380"
     CDS             3890..>4290
                     /locus_tag="AC16VR_00380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR6009632.1"
                     /translation="MARGYPKIDLPVGCEPTDNIWEAMFTQRAIRYWQDKPVPRELLE
                     KVVAAGSKAPSGSNTQPWVFVVVDEPEKRAELGAALRGIFDAAEPLQQLISAGEQSDD
                     KTERLMLKGARAFFSHLEKAPAMIIPSLYGLS"
BASE COUNT          658 a         1391 c         1479 g          762 t
ORIGIN      
        1 gcacgtgttg tagaaggcgt tcaccgcgcc gctgtccgca tgaacgagca ccacgggctg
       61 gcccgcggtc tcccagaggc ggaaacagcc gggttccgga atctcgtcga tgtgaccggc
      121 gagcagccac gactttcgcc agatgtgctg ctgctccagg tcgtagaagc gctgatcggt
      181 gtaacgaccg cccggcaggt cgggcagtga cggaaaacct tcgggcggtt cgcttcgtgc
      241 cgcctcgtac tccatgagct ggcgcagctc gtcgatttct ttctggttca tggattcagc
      301 ctctctcgtc gtgccatcga tggttctacg atgcttcgtt ttagcgggcc aggtgcccgc
      361 cgtccacggt cagggtggtg cccgtgacga agccggcctc gtccgagcac agccacatgg
      421 ccgcgtttgt cacttcctct agctgcccga gccgcccgat cggatgctgt gagatcagat
      481 cggcctccag gccgggcgtc tgggtgattc cccaggcgag catgtcggtg tagatcacgc
      541 ccggcgccag ggcgttgatc ctgatgttct gctcggcgta gtccagcgct gcggccttgg
      601 tgagcccgat gatggcgtgc ttggacgcca cgtaagccac gccgcccggc gagccgccga
      661 ttcccgcgat ggacgacatg ttgacgatgg cgccgccccc gcgctcgagc atggccggga
      721 tggcgtgctt tacgcagaga aaaacgcctt tgacgttgat ctccatggtc tcgtcccagg
      781 cgtcctcgga atactctgcc gccttggcaa aggcgtcgcc gggaacgccg gccacgttgt
      841 aggcccagtc gagcccgccg aatcggtccc gggcagcggc cacgatgcgc gcaacgtcct
      901 cgctcacgct gacgtcggcc tggacgaaca cggccgtgcc gccggcggct tcgatctccg
      961 cgagggtctc ttcgccttcc gcctggcgtc gtccggacgc tacgaccctg gccccttccc
     1021 gggcgaaggc gacggccgcg tgcttaccca gaccggacgt ggcgccggtg atcatgacga
     1081 ccttgccgtc gaagcgtccg ctcatcgttt gctccccaag cactcttgcg ccaattgtgc
     1141 ccgaggccct gcgtacccgc tatctcatca aggccggatt ccacggcccc gggcgagagt
     1201 ctgccggagc gcccggcggt tcagctttgc gctgccctgt gtcaggctac gtgctctgag
     1261 ttcccggggc gggtcggact tgccgaaagt ggtcaaagcg tcggtgtggg tcgtcgggct
     1321 gctggtcgtt gcggccggag gctactatgc gtacctgcag ctcgtcggca atccccgggt
     1381 gatgaccgaa atccgggccg agccggacgg cgagcgggca cggcgcgtca tgctcatcga
     1441 cctgcccgac ggccggaccc tgccggtcaa ctacctgcgt gaggacgaca tggtctacat
     1501 gggggtggac gggttgtggt ggcggcagtt tcgccagggc gatgtgcccg ttgagctgct
     1561 catccgtggt gaagatctgc agggccacgc ccgtgccgtg ctcgacgatc ccgaccggac
     1621 ccgcagggtc ttcgcccggc tccgacccac cgcgccggac tggcttccgg gccggatggg
     1681 cggcgtgctg gtcgagatcc ggctgcaggc ggcgactctc gatgaggagg actgacgtgg
     1741 ggacctgcgt ctactggcag cccgggtgaa ccagctgtct ccgggtgaag gagtttctat
     1801 cggcccgtgc ggtcgagttc gtttccatca acgtgctgga ggaccgggaa gcgcgtgagc
     1861 gccttgcccg tgcgggtatc cggaccctgc ctgtggtttc ccgcgacgac gctttcgtgc
     1921 ccgggatgga tctcgcgcgg gtcgccgaat tcctgggcct ggactatcag ggcggcaaca
     1981 tcctgccggc agacgtgctc gtcagccggt atcaccgggt gctcgatgcc gcggtgcgat
     2041 acgtggggca gataccgccc gcccgcctgc acgacacgct gccccatcgg gaccgttcct
     2101 accttgccct gggcaatcat ctcgttcaga tcgcggtgga tttcctgtcg gtgacccggg
     2161 gtgctgcgct caccggccgg cttgccgagt cggtgccgga gactgagctg gagccgccag
     2221 cactaatggc cgccgccgcc caggcgaagc gcggcctggc ggactggctg gcggcagcgg
     2281 acagtgccga gctcgcttca cctgtggtga cgtacttcgg ttcccagacg ctccatcagg
     2341 tgctggaacg ggcggtgtgg cactcggccc agcatgggcg tcagctgatg atggttctgc
     2401 aacggcttgg gatctcgccg gacgggccgc tgacggacga ggatttcgca gggctgccca
     2461 tgccggagga ggtctgggac ggctgaccgc cgcggcggtg gtgcgtgggg ttatgatgat
     2521 ggcgggcgtc cggtgacggt cgcccagcgg cgagtcgaac ggggaggaga acgggggaat
     2581 gcagcttcca accgacgagg aatgggcccg cgcgtgcgcg gcggatggcg agttcggtct
     2641 ggcggcccgg cactggacgg ggggtctcac cctcgccgtc ggggaggcgg agctcagcct
     2701 tcgcctgacg gacggcgccg tgtctccggg cagggccgaa tcggccgaag gtctgctgac
     2761 ctacgcgggc cctgccgacg tatgggagca agtgctcgcg gcccagccgc cgcggtttca
     2821 caacgacatc atggccaacc tgaccgtggg gggcgggctc aaccgccagg tcgacccggt
     2881 ggttcacgct cagtactacc cggcagtgat gcgcgccgtg gagctgcttc ggccggcgga
     2941 gccgccgttc atgcccatgc gcgacgaggc ccgaccgccc ggggccttcg acgcccccgt
     3001 ggggcgctat gtccatctgg agctggaggg ccacgatcac cggatctact tcgaggaggc
     3061 ggggcagggc atccccctgc tgatgcagca cacggccggc tgccacggca gccagtggcg
     3121 ccacctcttc gagattcccg agatcaccag ccgcttccgc ctcatcgcct acgacctgcc
     3181 gtatcacggc aagtcggtcc cccccgtcag ccgcgagtgg tgggcggagc gctacgagct
     3241 caagggagat ttcctgcgct cggtgccggt caagctggcc gaggcgctcg gtttggaccg
     3301 gcccgttttc atgggatgct ccgtgggcgg gctgctggct ctggacctcg cgctgcgaca
     3361 cgccgaggcg ttccgggccg tgatctccgt ggaaggcgcg ctcaacatcg agggcgatct
     3421 gggcgagctg gaggagctct ggcaccctca ggtgagcaac gagtacaagg cccgcctaat
     3481 ggacgggatc atggcgcccg cctcgccgaa ggcttaccgc aaggaaacga gcttcgttta
     3541 cgcctgcggg tggccgccgg tttttctggg cgatctgtac tactacatca acgacttcga
     3601 catccggcaa gcagcggggc agatcgacac cagccgggtg ggcgtgcaca tcctctccgg
     3661 cgagtacgac tacagcggca cctccgagct gggccggcag gcccacgagc tgatcccggg
     3721 ttccacctgg acggagatga agggggtcgg ccacttcccc atgaccgaga atcccgtgcg
     3781 gttcgtcgag tacctggaac cgatactcgc ctcgatctcg gcttagtttc attcccgctg
     3841 gaaaactgct ctactctgaa ccatccggcc gagcagggaa ggggggagca tggcgagagg
     3901 atatcctaag atcgatctgc cggtgggttg cgagccgacc gacaacatct gggaggccat
     3961 gttcacccag cgggccatcc gctactggca ggacaagccc gttcccagag aattgctgga
     4021 gaaagtcgtg gccgccgggt ccaaggcgcc gagcggctcg aatacccagc cgtgggtgtt
     4081 cgtggtggtc gacgaacccg aaaagagggc cgagctcggg gcagcactgc gggggatttt
     4141 cgacgcagcg gagcccttgc agcagctgat ttccgccggg gagcaatccg acgacaagac
     4201 cgagcggctg atgctcaagg gcgccagagc gtttttctcc catctggaga aggcgcccgc
     4261 catgatcatt cccagcctct acggcttgtc
//
LOCUS       DBNHQR010000018         1864 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1268629, whole genome shotgun sequence.
ACCESSION   DBNHQR010000018 DBNHQR010000000
VERSION     DBNHQR010000018.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1268629"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..1807
                     /gene="nadB"
                     /locus_tag="AC16VR_00385"
     CDS             188..1807
                     /gene="nadB"
                     /locus_tag="AC16VR_00385"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016568828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HIR6009633.1"
                     /translation="MGQRFSTDILVIGGGAAGLSAALHLAEHARVTVLCKGDVESAST
                     HWAQGGIAAVTDPDDTYEAHIQDTLNAGAGLCDHSVVAQTVERAPQIISQLVQWGIEF
                     DRSENEYHLTREGGHSHRRILHVADATGKALTQTLTDKVLDHPNIELFTDRVAIDLIN
                     LSKFGRHPNRCAGAYVLNRTTNHVEVFQAHFVILATGGASKVYLYTSNPDSSTGDGIA
                     MAWRAGCRVANMEFNQFHPTCLFHPHAKSFLISEAVRGEGGVLCLPSGERFMERFDPR
                     LELAPRDIVARAIDHEMKRLGLDCVYLDISHRPRSFIKSHFPTIAQRCADFGIDMANE
                     PIPVVPAAHYTCGGVVIDGNGASDVPGLYAVGEVSCSGLHGANRLASNSLLECLVFGE
                     AAARDIKRRLDEPRWDVNVPQWDESQVVDSDEDVIIAHNWEELRRFMWDYVGIVRTDK
                     RLQRAQRRIDLLKQEVHEYYSNYRVTNDLIELRNLIHVAELIVRSALVRKESRGLHYN
                     LDHPETMSRADDTILFPGSMDRLDVLQPVFS"
BASE COUNT          352 a          623 c          563 g          326 t
ORIGIN      
        1 tgctgatagc ggctgaacaa aagatcgaac gcgtagcggt cacccttctg gactctcctt
       61 accagttcct tgtcggtatc ttttgccact tagacccggt tcgaccccgg tgttccccaa
      121 gaggcccgat attctgccga ttgcgtcccg tttatactag ttggctaact gattccgggc
      181 ggtggacatg ggccagcggt tctccacaga cattctcgtc atcggcggcg gcgcggccgg
      241 cctgtcggcc gctctgcacc tcgccgagca tgctcgtgtt accgttctgt gcaagggtga
      301 cgtcgagagc gcttccactc actgggctca gggcggcata gccgcggtga cggatccgga
      361 cgacacctac gaggcccaca tacaggacac gctgaacgcc ggcgccggtc tgtgcgatca
      421 cagcgtcgtg gcgcagacag tggagcgcgc gccccagata atcagccagc tggtgcagtg
      481 gggcatcgag ttcgatcgca gcgagaacga gtaccacctg acccgcgaag gtggccactc
      541 tcatcgccgc attcttcatg tcgccgacgc caccggcaaa gcgctcaccc aaaccctcac
      601 cgacaaggtc ctcgaccacc cgaacatcga gctcttcacg gatcgggtcg ccatcgacct
      661 gatcaacctg agcaagttcg ggcgccaccc aaaccgctgc gccggcgcct acgtgctcaa
      721 ccgaaccacc aaccacgtgg aggttttcca ggcccacttc gtgattctgg cgaccggcgg
      781 cgcgagcaag gtgtatctgt atacgagcaa cccggacagc tccacgggcg acggcatcgc
      841 catggcgtgg cgggccgggt gccgcgtggc gaacatggag ttcaaccagt tccaccccac
      901 ctgcctgttt cacccccacg ccaaatcgtt cctcatctcc gaggcggtcc gtggggaagg
      961 cggcgtgctg tgcctgccct ccggggagag attcatggag cgtttcgatc cccggctgga
     1021 actcgccccg cgcgacatcg tcgccagagc catcgaccac gagatgaagc ggcttgggct
     1081 ggactgcgtc tacctggaca tcagtcaccg gccgcgctcg ttcataaaga gccactttcc
     1141 taccatcgcc cagcgttgcg ccgacttcgg catcgacatg gcaaacgaac ccatccccgt
     1201 cgtgccggcc gctcactaca cctgcggggg cgtggtcatc gatggcaacg gcgccagcga
     1261 cgtgcccggg ctatacgccg tgggcgaggt gagctgctcc gggctgcacg gagcaaaccg
     1321 gcttgcgagc aactcgctgc tcgaatgcct ggttttcggg gaagccgccg cccgggacat
     1381 caagcgccgc ctcgacgagc cgcggtggga cgtcaacgtg ccgcagtggg atgagagcca
     1441 ggtggtcgat tccgacgagg acgtcatcat cgcccacaac tgggaggaac tcaggcgctt
     1501 catgtgggac tacgtcggca tcgtcaggac ggacaagcgc ctgcagcgcg cgcagcggcg
     1561 gatcgacctc ctcaagcagg aggtgcacga gtactacagc aactatcgcg tcaccaacga
     1621 cctgatcgag ttgagaaatc tgatccatgt cgccgagctc atcgtgcgct cggcgctggt
     1681 ccgaaaggag tccaggggcc tgcactacaa cctcgaccat cccgagacga tgagtcgggc
     1741 ggacgacacc atcctgttcc cgggatcgat ggaccgtctg gacgtgcttc aacccgtctt
     1801 cagctgatcc gtccggtctc ggaccgcggc tttgagaaac cggcgcaagg cggcgaagtc
     1861 cgcc
//
LOCUS       DBNHQR010000019         4900 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1015082, whole genome shotgun sequence.
ACCESSION   DBNHQR010000019 DBNHQR010000000
VERSION     DBNHQR010000019.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4900)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4900)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4900
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1015082"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            433..1890
                     /locus_tag="AC16VR_00390"
     CDS             433..1890
                     /locus_tag="AC16VR_00390"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR6009634.1"
                     /translation="MRKSRHTQWTVNPSRAGSQGLILAMIFSGFSLAGLQAFAAKPNP
                     ICGNGICEQRESKSCPLDCADDPGEPPEPPPSTGVPSCGCSGAGLSPAQAKAGRSYVR
                     DLKVNDPVSGEVTSPYEVRLPSSYDVNSPGGFPVLVYLHGWGGSFRSLPASAARHAKN
                     NGYILVTPTGYGDGGRNSWNGYRSARLPVCALGDGYLASDCDAGPPELDSANGPSTCV
                     DLDNAGHDYCYDSCRTLYGAGAEPGNCPVWDGSQGDNTWGAEYNGDYTCHWTTCRDSV
                     GQIAALLDEIEQDYCVDRSMIWVTGCSNGGMMVYELAKDARTRTRFAGFMPQVGAPHP
                     GFRQYDPASELVDRPRHLLGFWGETDRTVPGRANFPGTSPQVALDTSFNGWLYVPASD
                     IGAEWASMHDAAPLPVEEADAYARAYSNSLDCQVWQAQDEGETPGHASEVATCFFQGG
                     HSCPGFGGMEAMMWDFAGRHAKTDADPAACSTSSP"
     gene            complement(1902..2288)
                     /locus_tag="AC16VR_00395"
     CDS             complement(1902..2288)
                     /locus_tag="AC16VR_00395"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6009635.1"
                     /translation="MIDINDRMAILDLCASYNYLFDTCAAKEWAATFTADGVFEGPAG
                     RAQGPEALAEFCRNTNAQYPGAMHFTDNHLFEQNGDTVRHKCFLSMQVPSPEGTQTLL
                     LTYEDELRRVDGEWRFASRLCQPLSA"
     gene            complement(2352..3272)
                     /locus_tag="AC16VR_00400"
     CDS             complement(2352..3272)
                     /locus_tag="AC16VR_00400"
                     /EC_number="3.8.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002043.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HIR6009636.1"
                     /translation="MIDVVRTPEERFRGLPDFPYDPRYAAWSGLRVAHIDEGPADAPV
                     ALLLHGEPTWSYLYRKMIPPLLEAGYRCVAPDHVGFGRSDKPVEDDWYVIERHVERIT
                     FLIESLDLTDITLFCQDWGGPTGLRQAVDMPERFERLVIMNTWLHHEGYEYSPAVRFW
                     REAATNRAWLGWTGGDLPCGAIVAGTLARSGLDRAAVKRAYDAPFAEGGRSKAGARRF
                     PWCLPFGEPEAGNAADQARCFEALKSWAKPAHFIFGERDPIFTTGWARQWSALLPNAT
                     LDLIPDASHFVQEEAGEEIVAAFLERTRST"
     gene            3455..3979
                     /locus_tag="AC16VR_00405"
     CDS             3455..3979
                     /locus_tag="AC16VR_00405"
                     /inference="COORDINATES: protein motif:HMM:NF025050.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6009637.1"
                     /translation="MLDKPTDRSIFQNAWVVDDVEAACMKWVEQMGVGPFFVNEYDNA
                     FEQIIYRGEPAKLDMIVAIAQAGPVQVEIIQPKVDRCAYRDSVPAGTDAFHHMCVWTD
                     DIDADSEYFESLGYPTANRGAVMDIEFAYYDTRPLMGCMLEVVTRGPGIIERFAQIAK
                     AAENWDGKDPIRYD"
     gene            3996..>4900
                     /locus_tag="AC16VR_00410"
     CDS             3996..>4900
                     /locus_tag="AC16VR_00410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-containing oxidoreductase family
                     protein"
                     /protein_id="HIR6009638.1"
                     /translation="MTTDGAVTAGERSHLTFCRICESLCGLRVSTRDGVVTDVRPDGD
                     HVATGGFACPKGLRQHHLYGSPDRLLRPRKRVGGDWRDVSWNEALGDIGGELARIRDE
                     HGPDAIAMYVGTAAGFGVLHPVFAQGFMDGLGSRNMYSSATQDCANKFAVAERIYGFP
                     FTQPFPDLLNTKLLVIVGANPMVSKWSFLQVPNPRRHLNAIRERGGRVVVVDPRRTET
                     AKAAGEHVFIRPNSDLYFYLAFLHELIAAGGVREDRVDAHMNGFEEVRELVEPWTAER
                     AAQHTGVSAEVLRQLVVDYVAADGAA"
BASE COUNT          821 a         1575 c         1671 g          833 t
ORIGIN      
        1 agcatgccgg ggggcagctc ggagacgttg gccggttgtc cttggccgaa cacgggatgg
       61 tcggccgggg cggcaaaggc ggcggctggg gtcagcacca tcaggagctt gacgagcctc
      121 agcgagccga ttctggggtg attgaccacg ggcaatctcc actgtctggg gtacgactat
      181 ggtagggagc ggcattgccc ggcatgtgcg gataggtgag ccgaatgcgc cgtcttggta
      241 gcgcaggtaa tgagaatgtt ggactgtgac gtgctcccca ttcccccgga aaccgcggtg
      301 ttcggggtga tggaaagggt tgcggacggt cgatgtagga aagatgtaat ccaggcgaag
      361 gagtgtctcg gaacggggcg ttaattgagt tagtctagac agtaaattga cattagcgac
      421 gaggaggtgg acatgaggaa atcccggcac acgcaatgga cggtcaatcc gagccgggca
      481 ggcagtcagg gcctgatcct cgccatgatc ttttccggat tctccttagc cgggctgcag
      541 gcgttcgccg ccaagccgaa cccgatctgc ggcaacggca tctgtgagca gcgcgaatcc
      601 aaaagttgcc ccctggactg cgcggacgat cccggcgagc ccccggaacc gccgccttcc
      661 actggggtgc catcgtgtgg ctgcagcggc gcggggctct ccccggccca ggcaaaagcc
      721 ggccggtcct acgtgcgtga tctgaaagtc aacgatcccg tctcgggcga ggtcaccagc
      781 ccctacgagg tccggctgcc gtcgagctac gacgtcaact cccccggcgg gttcccggtg
      841 ctcgtctacc tgcacggctg gggtgggagc tttcgatccc tgccggcgtc ggccgcccgt
      901 catgccaaga acaacggcta catcctggtg acccccaccg gctacggcga cggcggccgg
      961 aattcctgga acggctatcg cagcgcccgg ctgcccgtgt gcgcgctcgg ggacggatac
     1021 ctcgcctccg actgcgatgc cgggccgccc gagctggact cggcgaatgg gccgtccacc
     1081 tgcgtggatc tcgacaacgc cggccacgac tactgctacg acagctgcag gaccctctac
     1141 ggagcgggag ccgagccggg gaactgcccc gtctgggacg gcagtcaggg tgacaatacc
     1201 tggggcgctg agtacaacgg cgactacacc tgccactgga ccacctgtcg cgactcggtg
     1261 ggccagatcg cggcgctgct cgacgagatc gagcaggact actgcgtgga ccgcagcatg
     1321 atctgggtga ccggctgctc caacggcggg atgatggtct acgagctcgc caaggatgcg
     1381 cgaacgcgca cccggtttgc cggcttcatg cctcaggtgg gcgcgcccca tccgggtttc
     1441 cggcagtacg accccgcaag cgagctcgtg gaccggcccc gtcacctgct ggggttctgg
     1501 ggagagacgg accggacggt gcccggtcgt gccaactttc ctgggacgtc gccccaggtt
     1561 gctctcgaca cgagcttcaa cggctggctt tacgtgccgg cgagcgacat cggggccgag
     1621 tgggcatcga tgcacgatgc ggcgccgctg cctgtcgaag aagccgacgc ctacgcgcgc
     1681 gcctactcga acagtctcga ttgccaggtc tggcaggccc aggacgaggg tgaaacgcct
     1741 ggccatgcct ccgaggtcgc gacctgcttc ttccaggggg ggcactcctg tccgggcttt
     1801 ggcggcatgg aggcgatgat gtgggacttc gccggccgcc acgccaagac ggacgcggat
     1861 ccggcggcgt gctccacttc cagcccttag tggtcgtcag gctatgcgct cagcggctgg
     1921 cacaggcgtg aggcgaagcg ccactcgccg tccacccgtc tcagctcgtc ctcgtaggtg
     1981 agcagtaggg tctgggtgcc ctcgggggag ggaacctgca tgctcaggaa gcacttgtgc
     2041 ctgaccgtgt cgccgttctg ctcgaagagg tggttgtcgg tgaagtgcat ggcgcccggg
     2101 tactgcgcgt tggtgttccg gcagaactcg gccagcgcct cgggaccctg cgcccgtccg
     2161 gccgggcctt cgaagacgcc gtcggcggtg aacgtggccg cccactcctt cgcggcgcag
     2221 gtgtcgaaga ggtagttgta gctcgcacaa aggtcgagaa tggccatgcg gtcgttgatg
     2281 tcgatcatga gatctccctg aaggctcgtc tgggttcgtc aggccaggcc atgctagacg
     2341 cgcggtgccg atcacgtcga gcgcgtgcgc tccagaaatg cggcgacgat ctcctcgccg
     2401 gcctcctcct gcacgaagtg gctggcgtcg gggatcagat cgagggtggc gttgggcagc
     2461 agcgccgacc attgcctggc ccagcccgtc gtgaatatcg ggtccctctc gccgaaaatg
     2521 aaatgggcgg gcttggccca gctttttagc gcttcgaagc agcgcgcctg atcagcggcg
     2581 ttgccggcct ccggctcgcc gaatggaagg caccatggga accggcgcgc gcctgccttg
     2641 gagcggccgc cctcagcgaa tggcgcgtcg taagcgcgct tcacggccgc tcggtccagc
     2701 ccgctgcggg ccagcgtgcc cgcgacgatg gcgccgcagg gcaggtcgcc gcccgtccag
     2761 ccgagccagg cccggttcgt ggcggcctcc cgccagaagc ggacggccgg tgagtactcg
     2821 tagccttcgt gatgcagcca ggtgttcatg atcaccagcc gctcgaaccg ctccggcatg
     2881 tccacggcct gtcttaagcc cgtggggccg ccccagtcct gacagaacag ggtgatgtcc
     2941 gtgaggtcca gggactcgat gaggaaggtg atccgctcca cgtgccgctc gatcacgtac
     3001 cagtcgtcct ccacgggctt gtcagagcgg ccgaacccca cgtgatcggg agccacgcag
     3061 cgatagccgg cctccagcag cggcgggatc atcttgcggt acaggtagct ccaggtgggc
     3121 tcgccgtgca gcagcagcgc gaccggggcg tccgcggggc cctcgtcgat gtgggcgacc
     3181 cgcaggccgc tccacgcagc gtagcgaggg tcgtagggaa agtcggggag ccccctgaaa
     3241 cgttcttccg gcgtgcgaac gacgtcgatc atggaacccg tcctcctcag ctcgaggatt
     3301 gcccgttcaa ttgagcccaa gctactacgt acgccggcag tttcaaacct ggcgctgcag
     3361 cggtggttca cgatgacgtg cccccgcgct tggccgccgc gtgcggcgct tcgatgtgct
     3421 actctcagcg cgcactcgaa tctggaggga ggagatgctg gacaagccaa cggaccgcag
     3481 catttttcag aatgcgtggg tggtcgacga cgtcgaagcc gcctgcatga aatgggtgga
     3541 gcagatgggc gtcggcccgt ttttcgtcaa cgagtacgac aacgccttcg agcagatcat
     3601 ctaccgggga gagccggcca agctcgacat gatcgtcgcc atcgcccagg cgggcccggt
     3661 gcaggtggaa attatccagc ccaaggtcga ccgctgcgcc tatcgcgaca gcgtgcctgc
     3721 cgggaccgac gcgtttcacc atatgtgcgt ctggaccgac gacatcgacg cggactccga
     3781 gtacttcgag agtctcggct acccgacagc caatcgcggc gccgtaatgg acatcgagtt
     3841 cgcctactac gatacgcggc cgttgatggg ctgcatgctc gaggtggtca cgcggggacc
     3901 gggaatcatc gagcgcttcg cgcagatcgc gaaggccgcc gagaactggg acggcaagga
     3961 cccgatccgc tacgactgac cggcagcgct gactcatgac cacagacggg gcggttaccg
     4021 ccggcgagcg ctcgcacctc accttctgcc gcatttgcga gtcgctgtgc ggattgcgtg
     4081 tcagcacccg ggatggcgtg gtcacggacg tccgtccgga cggcgatcat gtggcaaccg
     4141 gcgggttcgc ctgcccgaag ggccttcgcc agcatcacct atacggctcc cccgaccgtt
     4201 tgctgcgccc tcgcaagagg gtgggcggcg actggcgcga cgtctcgtgg aacgaggccc
     4261 tcggcgacat cggcggcgag ctcgcgcgca ttcgggacga gcacgggccc gatgccatcg
     4321 ccatgtacgt gggcaccgcg gcggggttcg gcgtgcttca ccccgtattt gcccagggct
     4381 tcatggatgg gttgggctcg cggaacatgt actcctccgc gacccaggac tgcgccaaca
     4441 agttcgcggt ggccgagcgc atatacggct ttccgttcac gcagccgttc ccggatctgc
     4501 tcaacacgaa gctgctcgtg atcgtcggcg cgaatcccat ggtttccaag tggagctttc
     4561 tgcaggtccc caacccgcgc cggcacctca acgccatccg ggagcgcggc ggcagggtcg
     4621 tggtggtcga tccgcggcgc acggagacgg ccaaagcggc aggcgagcac gtcttcatcc
     4681 ggcccaacag cgatctctac ttctacctcg ccttcctcca tgagctgatc gcggcgggcg
     4741 gagtgcgcga ggatcgggtc gacgctcaca tgaacgggtt cgaagaggtg cgcgagctgg
     4801 tcgagccctg gacggccgag cgtgcggcgc aacacactgg cgtcagcgcg gaagtgcttc
     4861 gccagctcgt cgtcgactac gttgccgccg atggggccgc
//
LOCUS       DBNHQR010000020         6402 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1585466, whole genome shotgun sequence.
ACCESSION   DBNHQR010000020 DBNHQR010000000
VERSION     DBNHQR010000020.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6402)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6402)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6402
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1585466"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2550)
                     /gene="pepN"
                     /locus_tag="AC16VR_00415"
     CDS             complement(<1..2550)
                     /gene="pepN"
                     /locus_tag="AC16VR_00415"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HIR6009639.1"
                     /translation="MSSNVPQPVFLRDYEPPSYRTLDTRLSFDVRDGYTRVESQLSVE
                     RRAGGTDTVLRLDGQNLELLSVALDGRLLGGNEFVVDAESLTLFDLPERCEITIVTRI
                     EPEKNTALEGLYKSGSMYCTQCEAEGFRRITYYQDRPDVLARFTTTLEADASLYPVLL
                     ANGNPISDVPAGEGRRRVTWEDPFPKPSYLFALVAGDLAVMEDEFVTASGRRVALRIF
                     SEPQNIGQCGYAMEALKRAMRWDEETFGREYDLDILMIVAVEDFNMGAMENKGLNIFN
                     TKCVLASPDTATDAAHLRVESVVAHEYFHNWSGNRVTCRDWFQLSLKEGFTVFRDEEF
                     SSDMNFRAVKRIEDVNFLRSSQFPEDAGPMAHPVRPDSYIEISNFYTTTVYEKGSEVV
                     RMLRTLLGEERFRRGSDLFFDRYDGCAVTTDDFLGAMADVTALDLKQFRRWYEQAGTP
                     RLQVEEGWHEGELNLRIRQSCPPTPGQGSKKPFHLPVLIGLLDGAGRDLVDGGLDCSS
                     DAQVDVREGDRSLLVHVRDEITEIRIPGLPGRPAVSFLRGFSAPVRVEYPRDPETLAF
                     LALHDSDGFARWDALQTLVVREIDAMRSGGEPAAELVALLGNFIDPALAGGASAEQRF
                     LLSELLHVADEGYLFQALDDFEVEAVCDARDRLVVSVAANHAERWRALYEANAASGAY
                     APEPDGMSRRALRNTALGYLARVETGSALESLLEDHYRSADNHTDRSAAHRAVADVDG
                     LSDSFRSEMLDEFHRRWRAHALIVDLWFSVQASSPRSSAGTIRELESHPDFDGLNPNK
                     LRALHGAFGQMNHRNFHAQDGSGYDLLADRVLELDRNNPQIAARLLTPLTRW"
     gene            complement(2592..3470)
                     /locus_tag="AC16VR_00420"
     CDS             complement(2592..3470)
                     /locus_tag="AC16VR_00420"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR6009640.1"
                     /translation="MLLPLWLCFMRVIETDLDEDLSVLSRLLWERRVRHRIYEERGRQ
                     IVELADGAQAALVRDVYEALASGSLVVEAQPLPQRRPGLLAQIRRYPGLAVLVALAVA
                     LFPFTMPLAEGRVTYVASWLTIVDLIRLGSAPGTLVELVAEAAVWRWFAPMLIHFNAV
                     HLLFNCAVVIEMGRRIESRLGALWFWAIVLVVSLVANLGQYAMNANPLFGGLSGVAFG
                     LLGFVLAAGRLAPAVSQWQVPTAVAGGLLFFLVLFSTGVTEYFGLHVANAAHWFGLAA
                     GAVLGIVTGRLVGSGR"
     gene            3469..4503
                     /locus_tag="AC16VR_00425"
     CDS             3469..4503
                     /locus_tag="AC16VR_00425"
                     /inference="COORDINATES: protein motif:HMM:NF024869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIR6009641.1"
                     /translation="MQAYQIGATDEMHAVDEEVLNQVTEWLLADRPCWLATVVATWGS
                     SPRPVGSLLACNAEGQIVGSLSGGCVEDDLLEKLTSGELAAEHAQTFEYGVTAEESEK
                     FGLPCGGSLLIVIEPVAANAAYKAHMSHIRDQLAQRRCVKRTVDLKNRSFRTLQVPQH
                     EPLRWEADSQILEHTYGPRYQLFVIGAGMVSKYLAEMAQMLDYQVTVCDPREDLLNDF
                     GIPGVKLVADMPDDAIKAHASDPASAIVALTHDPRIDDMGMMQALTTDAFYVGAMGSA
                     RTSASRRERLAALELSEEQIGRLHAPIGLPIGSKTPPEIAVAILAEITAVRKARAESA
                     CAHLAQLSAG"
     gene            4510..5097
                     /locus_tag="AC16VR_00430"
     CDS             4510..5097
                     /locus_tag="AC16VR_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009613168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR6009642.1"
                     /translation="MRGGVVLLAAGSASRFGSDKRTHSLPDGRSLLGATIAKYARAFA
                     EIIVVLGPADRALAEELRRQFDPQTVTIVHAQAARLGMGHSLAAGIRAARGWDFAFVA
                     LGDMPFVQPQTLAALGRAMSEAGHDAIVQPFHEGHKGHPVGFGRRHFSALEALTGDEG
                     AREVVKTSASDLVPLEVDDPGVLQDVDTPADLAGD"
     gene            complement(5111..5998)
                     /locus_tag="AC16VR_00435"
     CDS             complement(5111..5998)
                     /locus_tag="AC16VR_00435"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011532967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HIR6009643.1"
                     /translation="MTGSFETVGLIGRSGKPRIRDSLRAVIDCMEANGRRVLLESDTA
                     RRMGKKLRHEQVSRQALGENCDLIIVVGGDGSLLGVGRDLAHARVPVLGVNRGGLGFL
                     ADVSPDQIAEKLAEVLKGDYVIEDHFLLNARIVRAGETLGHSPALNDVVVHMGGMARM
                     MEFSLWVDGEFVYDQRSDGLIVASPTGSTAYALSAGGPIMHPNLDAIVVVPMFPHTLT
                     SRPLVVTGESEIKILAAEDAEVTCDSQVDLALRSGDEVCISKFDHPLRLVYTLGHSFY
                     ESCRSKLDWATRLGGRNND"
     gene            6097..>6402
                     /locus_tag="AC16VR_00440"
     CDS             6097..>6402
                     /locus_tag="AC16VR_00440"
                     /inference="COORDINATES: protein motif:HMM:NF018165.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0009001 - L-serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - L-cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase domain-containing
                     protein"
                     /protein_id="HIR6009644.1"
                     /translation="MTSALDQRYSQVLTEALSQSAHGAGEPDDLWTLMRGEAHSKAAE
                     EPILGSYFHATILNHESFGSALSFRLASKLDNPMLPTMLIRDVIQEAMADDPAILRSA
                     "
BASE COUNT         1241 a         2106 c         2016 g         1039 t
ORIGIN      
        1 ccagcgcgtg agcggcgtca gcagccgcgc ggcgatctgc gggttgttcc ggtcgagttc
       61 cagcacccga tccgccagga ggtcgtagcc ggatccgtcc tgggcatgga aatttcgatg
      121 gttcatctgg ccaaacgccc cgtgcaaagc gcgcagcttg ttgggattca ggccatcgaa
      181 gtcagggtgc gattcaagct ccctgatggt gccggcgctg ctgcgcggac tgctcgcctg
      241 aaccgaaaac cacaggtcga cgatgagcgc gtgtgcccgc cagcgccgat gaaactcgtc
      301 gagcatttca ctgcgaaaag aatcgctgag accatcgacg tcggcgacgg cccggtgggc
      361 ggcgctgcgg tccgtgtggt tgtcggccga ccggtaatga tcctcgagca acgactccag
      421 agcggacccg gtctcgaccc gggcaagata cccgagggcc gtgttgcgca gggcccggcg
      481 ggacatgccg tcgggctcgg gcgcgtaggc acccgaggcc gcgttggcct cgtaaagcgc
      541 gcgccaccgc tcggcgtgat tggccgccac cgacaccacg agccggtctc gggcatcgca
      601 aacggcttct acctcgaagt cgtcgagcgc ctggaagaga taaccctcgt ccgcgacgtg
      661 cagtaactcc gacagcagaa agcgctgctc ggcgctggcc ccaccggcca gcgcaggatc
      721 gatgaagttg cccagcagcg ctacgagctc ggcggcgggt tcgccgccgg agcgcatggc
      781 gtcgatctcc ctgaccacca gcgtctgcag cgcatcccag cgcgcaaaac catcggagtc
      841 gtgaagagcc agaaaggcaa gcgtctcggg gtcacgggga tattccactc gaaccggagc
      901 ggagaacccc cgcaggaaac tcaccgccgg acgcccgggc agaccgggga tgcgaatctc
      961 ggtgatctca tcccttacgt gaacgagcag cgatcggtcg ccctcgcgca catcgacctg
     1021 ggcgtcgctc gagcagtcca ggcccccatc gaccaggtcg cggccggcac catcgagcag
     1081 cccgatcaga acgggaaggt ggaagggctt cttgctgccc tgcccgggcg tcggcgggca
     1141 ggactgccgg atgcgcaggt tcagctcgcc ctcgtgccag ccctcctcca cctgcaatcg
     1201 tggcgtcccg gcctgctcgt accagcgccg gaactgtttc agatcgaggg cggtcacgtc
     1261 cgccatggcg cccagaaagt cgtcggtcgt taccgcacag ccgtcgtagc gatcgaagaa
     1321 gaggtccgaa ccacggcgga accgttcctc tccgaggagg gtccgcagca ttcggaccac
     1381 ctcgctgccc ttctcgtaga ccgtcgtggt gtagaagttg gagatctcga tgtaagagtc
     1441 gggccgcacc gggtgcgcca tggggccggc gtcctcggga aactgcgacg aacgcagaaa
     1501 attcacgtcc tcgatgcgct tgaccgcccg gaagttcatg tcggaggaaa actcctcgtc
     1561 ccggaaaacg gtgaatcctt ccttcaagct cagctgaaac cagtcgcgac aggtgacgcg
     1621 gttgccggac cagttgtgaa aatattcgtg agccacgacg ctctcgacac gcagatgggc
     1681 ggcatccgtg gcggtgtccg gcgaagccag aacgcatttg gtgttgaaga tgttcaggcc
     1741 cttgttctcc atggcgccca tgttgaaatc ttccaccgcc acgatcatga ggatgtcgag
     1801 atcgtactcc ctgccgaacg tctcctcgtc ccagcgcatg gcgcgcttga gcgcttccat
     1861 ggcgtaaccg cactggccga tgttctgtgg ctccgagaag atccgaagcg ccacgcgacg
     1921 tccggaagcg gtcacgaact cgtcttccat gaccgcgagg tcgccggcca cgagcgcaaa
     1981 gagatagctc ggcttgggaa acggatcctc ccaggtcacc cggcggcgcc cttcgcccgc
     2041 cgggacgtcg gagatgggat ttccgttggc gagcaggacc gggtagagcg atgcgtctgc
     2101 ttccagggtc gtggtgaagc gcgcgagaac gtcgggacga tcctgatagt aagtgatccg
     2161 gcggaacccc tcggcttcgc attgggtgca gtacatgctc cccgatttgt atagaccttc
     2221 caacgccgtg ttcttttccg gctcgatacg ggtcacgatc gtgatctcgc agcgctccgg
     2281 caggtcgaag agggtcagcg actccgcatc gaccacgaac tcgttgccac ccagcaaccg
     2341 gccatccagg gccaccgaga gcagctccag gttctgacca tccagccgta gcaccgtgtc
     2401 cgtgccgcct gcgcggcgct ccacgctcag ctggctctcg acgcgcgtgt agccatcgcg
     2461 tacgtcgaag ctcagccggg tatccagggt ccggtaagac ggcggctcgt agtcccggag
     2521 gaacacgggt tgagggacgt tgctactcat cggcaaagct ccatatgcaa ccgttgacgg
     2581 cctcgcgccg atcagcgacc ggagccgacc agacgacccg tgacgatgcc gagaactgct
     2641 ccggcggcga gtccgaacca atgggccgcg ttggccacgt gaagaccgaa atactcggtc
     2701 acgcccgtgc tgaacagcac caggaagaac agcagcccac ccgcgacagc ggttggcacc
     2761 tgccattgag acacggcggg cgccagacgg ccggccgcca ggacaaagcc caacaggccg
     2821 aaggcgacgc cggaaagccc gccgaacagg ggattcgcgt tcatcgcgta ctggccgaga
     2881 ttggccacga gcgaaaccac caggacgatt gcccagaacc agagtgcccc aagccgactt
     2941 tcgatccgcc ggcccatctc gatgacgaca gcgcagttga acagaagatg cacggcgttg
     3001 aagtggatca gcatgggcgc gaaccagcgc cagaccgcgg cctccgccac cagctcgacc
     3061 agcgtcccgg gagcggatcc aagccgaatc agatccacga tcgtcagcca gctggcaaca
     3121 tacgttacgc ggccttcggc cagcggcatg gtaaacggaa acagcgccac ggccagggcg
     3181 accaggaccg caagccccgg gtacctgcgg atctgcgcga gcagccccgg acggcgctgc
     3241 ggcagaggct gtgcctcgac gaccagagaa cccgaagcca gcgcctcgta gacgtcccga
     3301 accaatgcgg cctgggcacc gtccgccagc tcgacgatct ggcgcccgcg ttcctcgtag
     3361 atgcgatgac gcaccctgcg ttcccagagc aggcgggaca gcaccgagag atcttcgtcc
     3421 agatccgtct cgatcacgcg catgaagcac agccacagtg gtagtaacat gcaagcctac
     3481 caaatcggcg caacggacga gatgcacgca gtagacgagg aagttctcaa tcaggtgacc
     3541 gagtggctgc tggccgatcg cccctgctgg ctggcgaccg tcgtggccac ctggggatcc
     3601 tctccccgcc ccgtcgggtc gttgctcgcg tgcaatgccg aagggcaaat cgtgggatcg
     3661 ctgtcgggcg gttgcgtcga ggacgacctt ctcgagaagc tcacgagcgg ggagctcgct
     3721 gccgagcacg cgcagacatt cgagtacggt gtcaccgcgg aggagtcgga gaagttcggc
     3781 ctgccgtgtg ggggctctct gctcatcgtg atcgagcccg tggctgcgaa cgccgcctac
     3841 aaagcccaca tgtcgcacat ccgtgaccag ctcgctcagc gccgctgcgt gaaacgaacg
     3901 gtggatctga aaaatcgctc cttccggacc ttgcaagtgc cccagcacga gccgctgcgc
     3961 tgggaggccg attcccagat tctcgagcac acctacggcc cccgctacca gcttttcgtc
     4021 atcggtgccg gcatggtgtc gaagtacctc gcggagatgg cccagatgct ggactaccag
     4081 gtcaccgtct gcgacccgag ggaagatctg ctgaacgact tcggcatccc gggcgtgaag
     4141 ctggtggccg acatgcccga cgacgcgatc aaggcccatg ccagcgatcc cgcttcggcg
     4201 atcgtcgcgt tgacccacga cccgcgaatc gacgacatgg gcatgatgca ggcgctgacc
     4261 accgacgcct tctacgtggg ggcaatgggc tccgcgcgga cgtcggccag ccgtcgggag
     4321 cgactcgccg cgctggagct gagcgaggag cagatcggac ggctgcacgc gcccattggc
     4381 ctgcccatcg gcagcaagac gccgccggag atcgccgtcg ccattcttgc ggagattacc
     4441 gccgtgcgta aggcgcgggc cgaatccgcc tgcgcacacc tggcccagct gagcgccggc
     4501 tgaatcgacg tgcgcggtgg tgtggtgctg ctggccgcgg gttccgccag ccgcttcggc
     4561 tccgacaaga gaacgcattc attgcccgat ggccgctcgc tgctcggcgc cacgatagcc
     4621 aaatacgcca gagcattcgc cgagataata gtggttctcg ggcccgcgga ccgcgccctt
     4681 gccgaagagt tgcgacggca gttcgatccg cagaccgtga ccatcgtcca cgcgcaggct
     4741 gcgcgtcttg gcatgggtca ttcccttgcg gccggaatcc gcgcagccag gggatgggat
     4801 ttcgccttcg tggctctcgg cgacatgccc ttcgtccagc cacagaccct ggcggccttg
     4861 ggccgcgcca tgtccgaggc agggcacgac gccatcgttc agccgtttca cgaaggccac
     4921 aaaggtcatc cggtcgggtt cggccgccgg cacttcagcg cgcttgaagc gctcaccggc
     4981 gacgaaggcg cccgcgaagt ggtgaagacg tcagccagtg atctggttcc cttagaggtc
     5041 gacgaccccg gcgtgctgca ggacgtggac actccggccg atctcgccgg ggactgatca
     5101 gccgggcggt tcagtcgttg ttccggccgc ccagtcgggt cgcccagtcc agcttgctgc
     5161 gacaggactc gtagaagctg tggcccaggg tgtagaccag ccgcagaggg tggtcgaact
     5221 tcgatatgca tacctcgtcg ccggagcgca gcgctaaatc cacctgtgaa tcgcaggtga
     5281 cctccgcatc ttcggccgcg aggatcttga tctccgactc tcccgtcacc accaggggtc
     5341 gagacgtcag cgtgtgtgga aacatgggaa ccaccacgat ggcgtcaagg ttcggatgca
     5401 tgatgggacc accggcggaa agcgcgtagg ccgtcgagcc cgtcggggat gcgacgatga
     5461 gcccgtcgga gcgttgatcg tagacaaact cgccgtccac ccagagcgag aactccatca
     5521 tgcgggccat accacccatg tggacgacca catcgttgag ggcgggcgaa tggccgagcg
     5581 tctcgccagc ccggacgatg cgggcgttga ggagaaagtg atcttcgatc acgtagtcgc
     5641 ctttgaggac ctcggccagc ttctcagcga tctgatccgg agacacgtcg gccagaaaac
     5701 ccaggccacc acggttcacg cccagcacgg gaacacgagc gtgggctaga tccctgccca
     5761 ccccgaggag gcttccgtcg cccccgacca cgatgatgag gtcacagttt tctcccagag
     5821 cctgacggga cacctgctcg tggcgcagtt tctttcccat tctccgcgca gtgtccgatt
     5881 cgagcagaac gcgcctaccg ttggcctcca tgcagtcgat gacggcacga agactgtcgc
     5941 gtatgcgggg tttgcccgaa cgaccgatca ggccgacagt ctcgaaagaa ccggtcatga
     6001 cagtccagag tgaaggaaaa ccccgacact acacacgccg ccggttgccg acaatgcttg
     6061 caataccgtc atgaatacat agtctgaacg gtcatcatga ccagcgcact ggatcaacgt
     6121 tactctcagg tcctcaccga ggctttaagc cagtcggcgc acggagccgg cgagccggac
     6181 gacctgtgga ctctgatgcg cggcgaagct cactcgaaag cggccgagga acccatactc
     6241 ggcagctact tccacgcgac gatcctgaat cacgaaagct tcggctccgc cctcagcttc
     6301 cggctcgcta gcaagctcga caatcccatg cttccgacca tgctgattcg ggatgtcata
     6361 caggaggcca tggcagacga ccctgcgatc ctgcggtcag ca
//
LOCUS       DBNHQR010000021        10471 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_64837, whole genome shotgun sequence.
ACCESSION   DBNHQR010000021 DBNHQR010000000
VERSION     DBNHQR010000021.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10471)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10471)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10471
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_64837"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="AC16VR_00445"
     CDS             complement(<1..451)
                     /locus_tag="AC16VR_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009645.1"
                     /translation="MWQPSFSKDLPEVAQRSFRNEQLTELTLPVAFTLLEGGIVGVIA
                     AKIFHVPPIVLAVITAAPMFGNISSFIWNRLATAKLKVRFANVVQILTMACVLAIALA
                     PVNQIGAVLLVAGMIMSRLLITGIITVRSVIWSLNYNRALRARTAGRL"
     gene            complement(485..1537)
                     /locus_tag="AC16VR_00450"
     CDS             complement(485..1537)
                     /locus_tag="AC16VR_00450"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003682 - chromatin binding [Evidence
                     IEA];
                     GO_function: GO:0003714 - transcription corepressor
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_function: GO:0140297 - DNA-binding transcription factor
                     binding [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HIR6009646.1"
                     /translation="MDRKKVVVQLPAISKAKAVEDRYALELEALAPLADIVEVDAQTP
                     AEFGEGASDADAIITSWGVKIDREVIGRLERCVVIGVGSVGVDMVDVDAATKAGIVVT
                     NVPDVFIEEVADHAMMLLLAAVRRTRLMGDLVRSDNWLQARPMLSKVPRLMGRTLGLF
                     SFGNVARCTARRARAFGLHVIAFDPYVSELAISDAGVEPVSFAELLERSDYLSVHAPL
                     NDETRHVLDGDAFARMKPGAVIVNTARGPIIKEDDLIKALENGQIGAAGLDVLEREPP
                     DPANPLLDMANVIITPHVASASTRMRPETRRRVGREVALVLRGKWPMSCVNPTVLPRA
                     SLERWQPYPTQRGPNR"
     gene            1655..2446
                     /locus_tag="AC16VR_00455"
     CDS             1655..2446
                     /locus_tag="AC16VR_00455"
                     /EC_number="4.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015297.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase family protein"
                     /protein_id="HIR6009647.1"
                     /translation="MRENALLAAWREGRQTIGCWLSMGNAYSAEALGQLGFDYVCIDL
                     QHGLVDYLDLTYMLPAVSRGTSTPLVRVPWNEPYEIMKALDAGAYGVIVPMVNNAEDA
                     ARAVDACRYPPLGSRSFGPIRGALYGGRGYAAQANEQMACIVMVETREGLDKLEEIVT
                     TPGVDGVYIGPADLALALGLPALGDTDVPEHVAEVTRILEVCHRHELPAGIHTSSLDY
                     CQKRLAAGFDFVTLGSDAGFMTRTAAQELAAARGTAEQEREKTGY"
     gene            2538..3353
                     /locus_tag="AC16VR_00460"
     CDS             2538..3353
                     /locus_tag="AC16VR_00460"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6009648.1"
                     /translation="MTGYEQILFEAQDSIAVLTLNRPERMNAWTYQMSRELSHAIGVC
                     NEDPAIGAIIITGAGRGFCAGADIEHNFKARLDGNDDRGDDATPWVRLLRQSKPIIAA
                     VNGASVGVGATMILPADIIVAADSAKFAMGFIKMGLVPELASSHFLVQRMGFGRASEM
                     CLTGKMYPASEAFAMGLVDHLVADDALMDKAMDLAQAIAANPGPQLRWVKQLLTENGC
                     ATDLDEVQRLEGKLLQQAFASPEHKEAVAAFLDKRTPDFIGARAAQGEAGGTA"
     gene            3431..4732
                     /locus_tag="AC16VR_00465"
     CDS             3431..4732
                     /locus_tag="AC16VR_00465"
                     /inference="COORDINATES: protein motif:HMM:NF014680.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HIR6009649.1"
                     /translation="MQARASYAAGQLQQAEAGQAERLVLEERLGERERDLVRLEERIT
                     SFENQLNSARSALDERTKELSDRQAAQAAVEARLEESRKGFAEKEAVFKESSEALKQE
                     FELLATKIFDQQGVKHQERLSSVLTPFKEQIVDFRKRVEQVYHTDTKDRASLLNEVRN
                     LQKASERINEEAENLTKALKGDVKVQGNWGEVVLERVLEESGLREGHEYFLQAARRSE
                     RGELKRPDVLIRLPDGKDVVVDAKVSLLAYEEALSAETDGARELALRQHLVSLRAHVK
                     RLGEQDYDQLSDVRSLDFVLLFVPIEAAFTMAMEHDHRLFTEAFEKRIVIVSPTTLMM
                     TLRIIHNVWRFEKQNRNAQDIARRAGALYDKLRGLIEDMDLLGKQLGTVEKTYQAAYG
                     KLASGKGNLVRQVERFRELGAQVKRPLPKSLLDEATDRSDD"
     gene            4846..5070
                     /gene="csrA"
                     /locus_tag="AC16VR_00470"
     CDS             4846..5070
                     /gene="csrA"
                     /locus_tag="AC16VR_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HIR6009650.1"
                     /translation="MLVLTRRAGESIMIDDDIELKVLKIRGSQVHLGIDAPKRSAVHR
                     KEVWLRINGEPKAAGREDKEDSSDPSRGVA"
     gene            complement(5101..5547)
                     /locus_tag="AC16VR_00475"
     CDS             complement(5101..5547)
                     /locus_tag="AC16VR_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009651.1"
                     /translation="MPAFSYCICTTVDARALQALLEASGQGSYKDEQPWLIAREILEE
                     AASQSARLPILFASGHPSEFSHWGFIESIDVVELHRGTWESRCAFSPLQPVNPIWTSI
                     DSVFLKPTGEQLRREALEGIAHHRYPLNEGELHPYAICETPAFILA"
     gene            5620..7236
                     /locus_tag="AC16VR_00480"
     CDS             5620..7236
                     /locus_tag="AC16VR_00480"
                     /inference="COORDINATES: protein motif:HMM:NF020354.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HIR6009652.1"
                     /translation="MASSIVPQLAANSPPPPDYYAENLRQLITHVHAHYGDVLRREEI
                     GYGRRIVACSVPAQRLYARLLSRRGPWIRIDKLRYREVSDVTAAVGELEEAGLVVRNG
                     RAPADALLGLLSTAERRELFAAIGDAPKARWINQCVARYSDAHVLSRLAGVHPWVSIR
                     DFPTFWLHQLLFFGDTRQDLSAFVLQDLGLVRYEPYELDPRHRQFANRERLDEYLRYL
                     ELSSLSYRLEEQPVLASWLSGAMWTEPASRWAQRSRDKTANRLGRWHERRGEYDQALA
                     SYARSTSHPARERTARILDRLGDAEGVQDVLHAMLSEPRSAEEEDFARRFGPNGRRKR
                     HAHRLERLPDGDGVRVEQLAVDLLTEGGGRGWHLENRFSLGLAGLAFWPVVFADVPGA
                     FVNPFQQGPVDLFWPDFVEQRAGILEVQLDKLATQGGLASVVMDTWRRKFGVANRLVS
                     WAHFDEDLLEAVLGQLPQGDLLRLADFTLRNLHRLRKGFPDLLVIRAPGDYEFVEVKG
                     PTDQLQPVQRIWLDALEELDLPARVLRFKA"
     gene            7233..9557
                     /locus_tag="AC16VR_00485"
     CDS             7233..9557
                     /locus_tag="AC16VR_00485"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HIR6009653.1"
                     /translation="MKDTLSVRFLAEFVHRRGDLHARLDGRARAEEGIQAQRKLQRGR
                     GPAYERERRVALTREVGGRAHLISGRVDGADLTARLVEEYKTTRADPELAHQKLGSAH
                     WAQVKLYGGMLVEELDGASDSQWTLRLVYCHPDTLATREFDQQLSGHELATFLDETLD
                     SYAGWVAMQLAHERNRNEAIARRDFPFPRYRPHQQAVARRVYRALRDREQLLLEAPTG
                     SGKSMALLFPAAKALAATDHQKIFYLTSRSTGSLAALDACERLDPDGDYLRRVHIVAR
                     ERTCPVEGVPCDPALCQYANGYYDRIHGALAELLERPVMDAPRVAEVAEAHRVCPHEL
                     SLDAAVWADVLVGDYNYVFDPMVRLQRFAEDPGIALLVDEAHQLSTRARDMLSLEVTR
                     SAVQQARVEQPPAAILRRVRSVERKLVEIASAQRRSGETSVPRPDPLIRAMARLVDEV
                     AGSDVDIAPYPATRSLLMTCYRWVRGESWYDEACCAHLGDAPDGDYRLRRVHLDPAAY
                     LKGVFDGFGGHVRFSGSVSPLPLYQRLHGTTGAPAERAGSPFDPEQLEVLVVDDVPTY
                     LAARARSLERLAALVETVVSANRGHYLVALPSFAYLDDLAWALAGRASLPDVAAQTPG
                     MPPEERDRFLERFGSGSPACIGLVVMGGVFAESVDFAGRLAGVICVGVGLPPPEPERA
                     RLQAHFAGVGEDGNAVAYQQPAMIKVLQMAGRLLRDPEDRGILCLVDDRFKDSAYGRF
                     FPAHWRPQAVRASQVAGRLEKFWQGAAGSPRLRA"
     gene            9557..>10471
                     /gene="pyk"
                     /locus_tag="AC16VR_00490"
     CDS             9557..>10471
                     /gene="pyk"
                     /locus_tag="AC16VR_00490"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:TIGR01064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HIR6009654.1"
                     /translation="MRRRRTKIICTIGPATANYPTLERLQAAGMNIVRLNMSHADHAS
                     AKKIINWIRTLNRKVQYPVAILLDTQGPEIRTGEINEPMDLSTGDVVTISVLPEDVER
                     SSIHVNYRELVDILQVGNRMTVDNGLLNFEVLEKKGQQLVCRVIDGGVLRSKSHVNLP
                     GVRVNLPSITPKDRRDVEFGIENDVDFIALSFVRSAEDVTELRGILGAKAKNIKIIAK
                     IEDQEGVTHVHDIVRAADGVMVARGDLGIETNIADLPNVQRRIVHSAAKWGKRSIVAT
                     HLLESMIENPIPTRAEVTDVANAIYEGAD"
BASE COUNT         1865 a         3301 c         3514 g         1791 t
ORIGIN      
        1 gcaggcgccc ggcggtgcgc gcccgcaacg cgcgattgta gttgaggctc cagatgacgc
       61 tgcggacggt gatgatcccc gtgatcagga gcctgctcat gatcatcccc gccaccagaa
      121 gaacggcccc gatctggttg acgggagcaa gcgcgatggc gagcacgcag gccatggtca
      181 gtatctggac cacgttagcg aaacggacct tcagcttggc agtcgcgagc cggttccaga
      241 tgaagctgga aatgttgccg aacatgggag cggcggtgat gacggccagg acgatgggcg
      301 ggacgtgaaa gatcttggcc gcgatgacgc cgacgatgcc gccttccagc agggtgaagg
      361 cgacgggcag ggtcagctcg gtgagctgct cattgcggaa gctgcgctga gcgacctccg
      421 gcaggtcctt cgagaaagac ggctgccaca cggggtcagc cagccgtccg gggtgatcgc
      481 cgcctcaccg gttggggccc cgctgggtgg ggtagggttg ccatcgctcg agagacgccc
      541 ggggcagcac cgtggggttg acgcagctca tgggccactt tcctcgcagc accagggcca
      601 cctccctgcc cacacggcgg cgggtctccg gcctcatgcg ggtagaagcg gaggcgacgt
      661 gaggagtgat gatgacgttg gccatgtcca gaagcgggtt ggcgggatct ggcggctcgc
      721 gttcgagcac gtccagaccg gcggctccga tctgcccgtt ctcgagggct ttgatcagat
      781 cgtcttcctt tatgatgggg cctcgggcgg tgttcacgat caccgcgccg ggcttcattc
      841 gagcaaacgc atcgccgtcg agaacgtggc gcgtctcgtc gttcagcggc gcgtgaacgg
      901 acaggtagtc ggagcgttcc agcagctcgg cgaaagagac gggctccacg cccgcgtcgc
      961 tgattgccag ctcgctcacg tacgggtcga aggcgatgac gtggaggccg aacgcccggg
     1021 ctcttcgcgc cgtgcaacgg gcaacgttgc cgaaagagaa caggcccagc gtccggccca
     1081 tgagccgagg caccttgctc agcatcgggc gtgcctgaag ccagttgtcg cttcgtacca
     1141 ggtcgcccat caggcgcgtg cgccgcaccg cggcgagaag gagcatcatg gcatggtcgg
     1201 ccacctcctc gatgaacacg tcgggaacgt tggtcaccac gatgccggcc ttcgtggcag
     1261 cgtccacgtc gaccatgtcg acgcccacgc ttcccacccc gatcaccacg cagcgctcga
     1321 gacggccgat cacctcccgg tcgatcttca cgccccacga ggtgatgatg gcatccgcat
     1381 cgctcgctcc ttcgccgaac tcggcgggcg tttgcgcgtc cacctcgacg atgtccgcca
     1441 gaggtgccag ggcctcgagc tcgagcgcgt agcggtcttc aaccgccttt gccttcgaga
     1501 tggcgggaag ctgaacgacc acctttttcc tgtccatcgt cgagctccgc cgggtgcaaa
     1561 tgagaacgcc gattttcgca cgccggcgtt tcgagtgtca gcagcattcc ttacctgtga
     1621 taaggtttgg gggtagatcc ggggagggaa gaacgtgcgc gaaaacgcat tgctggcagc
     1681 gtggcgggaa ggccggcaga ccattggctg ctggctctcc atgggaaacg cgtactccgc
     1741 agaggcgctg ggacagctcg gcttcgacta cgtgtgcatc gatctgcagc acggcctggt
     1801 cgactacctg gatctgacct acatgctgcc cgccgtgtcc cgaggcacat cgacgccgct
     1861 ggtgcgcgta ccctggaacg agccctacga gatcatgaag gcgctggacg ccggcgctta
     1921 cggcgtcatc gtgcccatgg tcaacaacgc cgaagacgcg gcgcgggccg tcgacgcctg
     1981 tcgctacccg ccgctgggca gccgctcttt cgggcccatt cgcggggcgc tttacggcgg
     2041 gcgtggctat gcggcccagg ccaacgagca gatggcctgc atcgtgatgg tggagacccg
     2101 cgagggcctg gacaagctcg aggagatcgt gaccacgccg ggcgtggacg gggtctacat
     2161 cggaccggcc gatctggcgc ttgcgctggg ccttccggcg cttggcgaca cggacgtgcc
     2221 cgagcacgtg gcggaagtca cccgcattct ggaggtctgt catcgccatg agctgccggc
     2281 gggcattcac acctcaagcc tggactactg ccagaaacgc ctggctgccg ggttcgactt
     2341 cgtcaccctg ggcagcgacg ccggtttcat gacgcgcacc gccgcccagg agcttgcggc
     2401 cgcgcggggc accgcggagc aggagcggga gaagacgggc tactgagtcc tgtctcgcca
     2461 gaccagccga tcgccccggg tggcttcacg gcccgattgc ctcatactta ggcccgaatg
     2521 aggcagagga gaccaaggtg accggctacg agcagatcct attcgaggcg caggattcca
     2581 tcgccgttct cacgctgaac cggccggagc gaatgaacgc gtggacctac cagatgagcc
     2641 gggagctctc gcacgccatc ggcgtgtgca acgaggaccc cgccatcggc gccatcatca
     2701 tcaccggcgc gggccgcggc ttctgcgcag gtgccgacat cgagcacaat ttcaaagcac
     2761 gcctcgacgg caacgacgat cgcggtgacg acgcgacgcc ctgggtacgc ctgctgcgcc
     2821 agtccaagcc catcatcgcg gcggtcaacg gggccagcgt gggcgtgggc gccaccatga
     2881 tcctgccggc ggacatcatc gtggcggccg attccgccaa gtttgccatg gggttcatca
     2941 agatgggtct ggttcccgag cttgccagct cccacttcct ggtccagcgc atggggttcg
     3001 gccgtgccag cgagatgtgc ctcaccggaa aaatgtaccc ggcgagcgag gccttcgcca
     3061 tggggctggt ggatcatctg gtcgccgacg atgcgctgat ggacaaggcc atggatctcg
     3121 ctcaggccat tgcggcgaat cccggcccgc agctgcgctg ggtgaagcag ctgctgaccg
     3181 aaaacgggtg tgccaccgat ctcgacgagg tccagcggct ggagggcaag cttttgcagc
     3241 aggcgttcgc cagcccggag cacaaggaag ccgttgccgc ttttctggat aagcggacgc
     3301 ccgacttcat cggggccaga gccgcccagg gggaggcggg aggcacggca tagatctctg
     3361 ggtccagggc gtcgggctct tcgcgggggg cgccgcgctc gcaagcgtcg tctggctgtg
     3421 gcgctgccgg gtgcaggcgc gagcctcgta tgccgccggg cagcttcagc aggccgaggc
     3481 gggccaggcc gaacgtctgg tgctcgaaga gcggctgggc gagcgtgagc gggatctggt
     3541 ccggctggag gagcggatca cctcgttcga gaaccagctg aactcggcac gctcagcgct
     3601 ggacgagcga acgaaggagc tcagtgatcg ccaggcggcc caggcggccg tcgaggctcg
     3661 actcgaggag tcgcgaaagg ggttcgccga gaaagaagcc gtcttcaagg aatccagcga
     3721 agcgctcaag caggagttcg agctgctcgc cacgaagatc ttcgatcagc agggggtcaa
     3781 acaccaggag cggctgtcca gcgttctcac ccccttcaag gagcagatcg tcgatttccg
     3841 caagcgggta gagcaggtct atcacaccga caccaaagat cgtgcctcgc tgctcaatga
     3901 agtgcgcaac ctccagaaag ccagcgagcg gatcaacgaa gaagccgaga acctcaccaa
     3961 ggccctcaaa ggtgacgtca aggtccaggg aaactggggc gaggtcgtgc tggagcgcgt
     4021 gctggaggag tccgggctga gggaagggca cgagtacttt ctgcaggctg cccgccgctc
     4081 ggaacgggga gagctgaaaa ggcccgatgt gctgatccgc ctgcccgacg gcaaagacgt
     4141 ggtggtggac gccaaggtct ctctcctggc gtacgaggaa gccttgagcg ccgagacgga
     4201 cggcgcccgg gagcttgccc tgcgtcagca cctggtcagc cttcgcgctc atgtgaagcg
     4261 tctgggtgag caggactatg atcagctcag cgacgtccgc tcgctggatt tcgttcttct
     4321 cttcgtgccc atcgaggcgg ccttcaccat ggccatggag cacgatcatc gcctgttcac
     4381 ggaggcgttc gagaagcgca tcgtcatcgt gagccccacg acgctgatga tgacgcttcg
     4441 gatcattcac aacgtctggc ggttcgaaaa gcagaaccgg aatgcccagg acatcgcgcg
     4501 gcgggcgggg gctctttacg acaagctcag aggtctgatc gaggacatgg acctgctcgg
     4561 caagcagctt ggcaccgtcg agaaaaccta ccaggcagcc tacggaaagc tcgccagcgg
     4621 caagggcaac ctcgtcaggc aggtggagcg cttccgtgag ctgggcgccc aggtcaagag
     4681 gccgctgccc aagtcccttc tggatgaagc gaccgaccgc tcagacgatt agccaaccag
     4741 ttctcctttc tcgccgctgc ccggccaaag ccgcgtgcac gtcacagaac ggatcagtca
     4801 atgtcagtag atttctacgg agaaaaggat tgcaaggaaa cctggatgct ggtcctcacc
     4861 cgtcgtgctg gggagtcgat catgatcgac gacgatatag agctcaaagt gctcaagatt
     4921 cgcggtagcc aggtgcatct gggaatcgac gcgccgaaac gttctgccgt gcaccgaaag
     4981 gaagtctggc tgcgcatcaa cggcgagccg aaagcggcag gccgcgaaga caaagaagac
     5041 tcgtcagatc ccagccgcgg cgtcgcctga gcggactcag ctcaactcga tcactctcct
     5101 ttaagccagt atgaacgctg gcgtctcgca gatcgcgtac ggatgcagct cgccttcgtt
     5161 gagcggatat cgatggtgcg caattccttc cagcgcctct cgtctgagct gctcgcccgt
     5221 gggcttcagg aagacgctgt ctatgctcgt ccagatgggg tttacgggtt gcagcgggct
     5281 gaacgcgcat cgtgattccc aggttccgcg gtgcagctcg accacgtcga tggattcgat
     5341 gaatccccag tgcgagaact cggaaggatg cccgctggcg aaaaggatgg gcaggcgggc
     5401 tgactgagac gcggcttctt ccagaatctc tcgagcgatg agccagggct gctcgtcctt
     5461 gtagctgcct tgcccgcttg cttccagcag cgcctgcagc gcgcgggcgt cgacggtggt
     5521 gcagatgcag taggaaaagg caggcattgg ggagctggcg agtactgtac atgtatacag
     5581 acctctttat aatccacagg cctcgttcga agcaaccgga tggcttcaag catcgtgcct
     5641 cagcttgccg ccaacagccc gccgccgccc gactactacg ctgagaacct gcgccagctg
     5701 atcacccacg tgcacgccca ctatggtgac gtgctgcgca gagaggagat cggctacggg
     5761 cgccgcatcg tggcctgctc ggttcccgcc cagcggctgt atgcccgcct tctgagccga
     5821 cgtgggccgt ggattcgtat cgacaagctt cgctatcgcg aagtgagcga tgtgacggcc
     5881 gcggttggcg agctggagga agccgggctc gtcgtccgca acggccgggc accggccgac
     5941 gcgctgctcg gtctgctctc tacggcggaa agacgtgagc tctttgccgc cattggcgat
     6001 gcgccgaagg ctcgctggat caaccaatgc gtagcgcgtt acagcgatgc tcacgttctc
     6061 tctcggctcg ccggtgtgca tccatgggtg agcattcgcg actttcccac cttctggctg
     6121 catcagctgc tgttcttcgg tgatacccgc caggatctca gcgccttcgt tctccaggac
     6181 ctgggcctcg tccggtacga gccctacgag ctcgatcccc ggcatcggca gttcgccaac
     6241 cgtgaacggc tcgacgaata cctgcggtat ctcgagctca gcagcctttc ctatcgcctg
     6301 gaagagcagc ctgtgctcgc atcatggctc tccggggcga tgtggaccga gccggcgtcg
     6361 cgatgggctc agcgatcgcg ggacaagacg gccaaccggc tgggtcgctg gcacgaacga
     6421 cggggcgagt acgaccaggc gctcgccagc tatgcgcgca gcaccagtca tccggctcgg
     6481 gagcgaacgg cacgaatact cgaccgtctc ggcgatgccg agggcgtgca ggacgttctc
     6541 catgccatgc tcagcgagcc gcgctcggcg gaggaagagg actttgcccg acggttcggg
     6601 ccgaacggcc ggcgcaagcg ccacgctcat cggctcgagc gcctacccga cggggatggt
     6661 gtccgggtcg agcagctggc cgtggacctg ctgaccgagg gcggtgggcg gggctggcat
     6721 ctggaaaacc gattctctct gggcctggcc ggcctggcgt tctggccggt ggtgttcgcc
     6781 gacgttcccg gcgccttcgt caacccgttc cagcaaggtc cggtggacct cttctggccg
     6841 gatttcgtgg agcagcgcgc cggcatcctc gaggttcagc tggacaagct agccactcag
     6901 ggcgggctgg ccagcgtcgt catggacacc tggcgccgca agttcggcgt tgccaaccgg
     6961 ctggtgagct gggcgcactt cgacgaggat ctgctggaag cggtgctcgg tcagctgcct
     7021 cagggcgatc tactgcgcct ggcggatttc accctgcgca acctgcatcg tctgcgtaag
     7081 gggttcccgg acctgctcgt gatccgcgcc ccgggggact acgagttcgt ggaggtcaaa
     7141 gggcccaccg atcagctgca gccggtacag cggatctggc tcgatgctct ggaagaactt
     7201 gacctgcccg ctcgcgtgct gcggttcaag gcatgaaaga cacgctttca gtgcgttttc
     7261 tcgcggagtt cgtgcatcgg cgcggtgatc tgcacgctcg cctggatggg cgggcccggg
     7321 cggaggaggg gattcaggct cagcggaagc tgcagcgcgg acgcgggccg gcatatgagc
     7381 gcgaacgtcg cgttgcgctc acgcgggagg tcgggggtcg agcgcacctg atatccgggc
     7441 gcgtggacgg cgccgatctc accgccaggc tcgtcgagga gtacaagacc acccgggccg
     7501 acccggaact tgcccaccag aaactcggaa gcgcccattg ggcgcaggtc aagctttacg
     7561 gcgggatgct ggtggaggaa ctcgatggcg catcggacag ccaatggacg ttgcggctgg
     7621 tgtactgcca tcccgacacg ctcgccaccc gcgagttcga tcagcaattg tccgggcatg
     7681 agctcgccac gttcctcgat gagaccctcg acagctacgc cggatgggtg gcgatgcagc
     7741 tcgcccacga gcggaaccgg aacgaggcga tcgcgcgtcg cgacttcccg ttcccccgct
     7801 atcgtcccca tcagcaggcg gtggcccggc gggtgtatcg ggcgttgcgc gaccgggaac
     7861 agctgctcct agaagcgcct acggggagcg gcaagagcat ggcgctcctg tttcccgcag
     7921 ccaaagcgct tgccgctacc gatcaccaga agatcttcta tctcaccagc cgctctaccg
     7981 ggtccttggc cgcactggat gcttgtgaga ggctcgaccc ggacggagat tacctgcgcc
     8041 gcgtgcacat cgtcgcgcgg gagcgcacct gtccggtgga gggcgtgccc tgtgatccgg
     8101 ccctgtgcca gtacgcgaac ggctattacg acagaatcca cggagcgctc gccgagttgc
     8161 tggagcggcc cgtcatggac gcaccgaggg tggcggaggt ggccgaagcc catcgggtgt
     8221 gtccccacga gctgtcactg gatgcggccg tctgggcgga cgtgctggtc ggtgactaca
     8281 actacgtctt cgaccccatg gtgcgtctgc agcggttcgc cgaggatccc ggcatcgctc
     8341 tgctggtgga cgaggcccat cagctgagca cgcgtgcccg ggacatgctg tcattggagg
     8401 tgacgcgcag cgccgtgcag caggcgcggg tcgagcagcc gccggccgcc atcctgcgcc
     8461 gcgtgcggtc cgtggagcgc aagcttgtcg agatcgcgag cgcccaacga cgctcgggcg
     8521 aaacgtccgt tccccgtccc gacccgttga ttcgcgccat ggcgcgcctc gtcgacgagg
     8581 tggcgggatc agacgtcgac atcgcgccgt atccggccac tcgatctctg ctgatgacgt
     8641 gttatcgatg ggtgcgtggt gaatcctggt acgacgaggc atgctgcgcg cacctgggag
     8701 acgcgccgga cggggactac cgcctgcgcc gggttcacct ggatccggcc gcttacctga
     8761 aaggcgtgtt cgacggtttc ggaggccacg tccgcttctc gggaagcgtg tctcctctgc
     8821 cgctctacca gcgccttcac gggaccaccg gggcgccggc ggaacgtgcc ggcagcccct
     8881 tcgatcccga gcagctcgag gtgctagtgg tggacgacgt gccgacttac ttggcggcga
     8941 gggcacggag cctcgagcgc ctggcagcgc tcgtggaaac ggtggtttcc gcgaatcgcg
     9001 gccactatct ggtcgcgctg ccgtcgttcg cgtacctgga cgatcttgcc tgggccctgg
     9061 ccggtcgggc atcgcttccc gacgtcgcag cgcagacgcc gggaatgcca ccggaggagc
     9121 gagaccgatt tctggagagg ttcggatccg gttcgcccgc ctgcatcggc ctcgtcgtga
     9181 tgggaggcgt tttcgccgag tccgtggact ttgccggacg cctggccgga gtcatctgcg
     9241 tgggcgtggg gctgcctccg cccgagcccg agcgggctcg gcttcaggcg catttcgccg
     9301 gcgtcggtga ggacggcaat gccgtggcct atcagcagcc ggcgatgatc aaggtcctgc
     9361 agatggccgg acgcctgctg cgtgatccgg aagaccgggg cattctctgc ctggtagacg
     9421 accgcttcaa agattcggcc tacggccgat tctttcccgc tcactggcgc ccccaagcgg
     9481 tgagggcttc ccaggtcgcc ggccgcctcg agaaattctg gcagggggcg gccggctccc
     9541 ctagactgcg cgcgtgatga gacgacgacg taccaagatc atctgcacca tcggtccagc
     9601 cacggccaac taccccacgc tggaaaggct gcaggcggcg ggaatgaaca tcgtccgcct
     9661 gaacatgtcc catgcggacc acgcctcggc aaagaagatc atcaactgga tccgcaccct
     9721 caaccgcaag gtgcagtatc cggttgccat cctgctcgat acccagggcc ccgagatccg
     9781 caccggcgag atcaacgaac ccatggatct gagcaccggc gacgtcgtga ccatcagcgt
     9841 gctgcccgag gacgtcgagc gcagctccat tcacgtcaac tatcgggaac tcgtggacat
     9901 tttgcaggtg ggcaaccgga tgaccgtcga caacggtctg ctcaacttcg aggtgctgga
     9961 gaagaaaggt cagcagctcg tctgccgggt gatagatggg ggcgttctgc ggagcaaatc
    10021 tcacgtcaac ctgccggggg tgcgcgtgaa cctgccatcg atcacgccca aggatcgccg
    10081 ggacgtggag ttcggcatcg agaacgacgt ggacttcatc gcgctgtcct tcgtgcgctc
    10141 ggcggaggac gtcaccgagc tgcgcggcat cctgggcgcg aaggcgaaga acataaagat
    10201 catcgcgaag atcgaagatc aggaaggggt gacccatgtg cacgacatcg tgcgcgcggc
    10261 cgatggggtg atggtggcca ggggcgatct cggcatcgag acgaacatcg ccgatctgcc
    10321 gaacgtgcag cggcgcatcg tgcactcggc ggcaaagtgg ggcaaacgct ccatcgtggc
    10381 gacgcacctg ctggagtcga tgatcgaaaa ccccattccc acccgggccg aggtgaccga
    10441 cgtcgccaac gccatctacg agggcgccga c
//
LOCUS       DBNHQR010000022         7036 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1712378, whole genome shotgun sequence.
ACCESSION   DBNHQR010000022 DBNHQR010000000
VERSION     DBNHQR010000022.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7036)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7036)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7036
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1712378"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..365
                     /locus_tag="AC16VR_00495"
     CDS             30..365
                     /locus_tag="AC16VR_00495"
                     /inference="COORDINATES: protein motif:HMM:NF037782.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009655.1"
                     /translation="MLLKVGRHLRPRPHFKLIIGRELGENRFLQGYRHQFTSLTAISH
                     KGPLALIDGDAGADDIELAARILARYGQGRDAARVEVIAAEPGGAETRLSVAPLAEAD
                     IGTDWHVGA"
     gene            352..636
                     /locus_tag="AC16VR_00500"
     CDS             352..636
                     /locus_tag="AC16VR_00500"
                     /inference="COORDINATES: protein motif:HMM:NF013379.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HIR6009656.1"
                     /translation="MSELETLDARGLLCPLPVIRTQEKVKGLPPGTVVEIVATDPGVM
                     QDIPAWCRVHGHELISMTEGEEEAAAEGKADADRLIRGGREIRVRIKVRQ"
     gene            836..2248
                     /gene="glnA"
                     /locus_tag="AC16VR_00505"
     CDS             836..2248
                     /gene="glnA"
                     /locus_tag="AC16VR_00505"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017517179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HIR6009657.1"
                     /translation="MAENTLKLIKDNDVKWVDLRFTDPKGKEQHTTIPAHRLDADALN
                     DGFMFDGSSISGWKSINESDMILMPDDSTPVIDPFRDDTTLNLRCDIIEPSTMQPYDR
                     DPRSLAKRAEAYLTSTGIGDTAYFGPENEFFVFDDVRWKVEMSGASYSIGAEEAAWES
                     HSEFEGGNLGHRPSVKGGYFPVPPVDSAADLRSAMCSAMMAMGLDVEVHHHEVATACQ
                     NEIGARFNTLVRKADEVQIYKYCVHNVADAYGKTATFMPKPLVGDNGNGMHVHQSIAK
                     GDTNVFHGDEYAGLSKTALYYIGGIIKHARAINAFANASTNSYKRLVPGFEAPVLLAY
                     SARNRSASIRVPYIQGGNPRAYRVEVRFPDSTGNPYLTFAAMLMAGLDGIQNEIHPGD
                     SFDKDLYDLPAEELEGVPTVCSSLEQALQALDEDRDFLKAGGVFSDGMIDGYLALKQQ
                     EVDYLNSTTHPAEFEMYYSV"
     gene            2362..2979
                     /locus_tag="AC16VR_00510"
     CDS             2362..2979
                     /locus_tag="AC16VR_00510"
                     /inference="COORDINATES: protein motif:HMM:NF024901.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HIR6009658.1"
                     /translation="MQLLATLKRIAFLSIISTPWLMLSTQSMAGTTIYKTVDEDGNVV
                     FTDTPPRADGPSETITLTSPNSFDPAEAGTEGSGRQLWIVDPDAEGEASGEFYGALTV
                     TSPADDESIRENAGNVTVTVKLEPPNLQPGHRIRLLLDGSPTGGTNTTGRFALSNVDR
                     GTHSLKAEVVDDAGKLIYAGPSSTFHLQRYSVLNAPKRPVNPINN"
     gene            3236..4360
                     /gene="glnL"
                     /locus_tag="AC16VR_00515"
     CDS             3236..4360
                     /gene="glnL"
                     /locus_tag="AC16VR_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HIR6009659.1"
                     /translation="MTQTHGNTAYNMPSLAKSDILDQLTGAVIVCDGSLRVCYMNPAA
                     ESLLNTSEHQSTGVQLAELLFDAAASPFADLAGIFTTEQSITKRAAQFQLRDGTEVIA
                     DLTASLEPNSGHLVIELQPLNRLLRINRDEHSFFSQQTTRTLIRGLAHEVKNPLGGLR
                     GAAQLLQRELDASELTEYTRIIIEEADRLTQLVDNMLGSNRQPEYADVNIHELLEHVV
                     RLVDAELGGRVKFARDYDPSLPPIRADRAQLVQALINIVRNAGQALGETANPSITLRT
                     RAVRQFTIGSTRHRVVLQIEIADNGPGIPEGMVDQIWFPMISGRADGTGLGLAITQSI
                     VGHHNGIIECVSRPGDTRFSVFLPVESNGEHIEQLENSQP"
     gene            4357..5757
                     /gene="ntrC"
                     /locus_tag="AC16VR_00520"
     CDS             4357..5757
                     /gene="ntrC"
                     /locus_tag="AC16VR_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HIR6009660.1"
                     /translation="MRNRVWVVDDDRSIRWVLERAMQQADIPIAAFASADHAMHHLQD
                     ERPVAVLTDVRMPGMDGMEFIGRIHEAYPELPVIIMTAHSDLDSAVNAYQSGAFEYLP
                     KPFDVDEAVAIVKRAMTFAQEEAPPPAPEQETAQEIIGQAPAMQEVFRAIGRLAHSNV
                     TVLIRGASGSGKELVARALHRHSPRAEGEFVALNMAAIPRELVESELFGHEKGAFTGA
                     NSRREGRFEQADGGTLFLDEIGDMPAETQTRLLRVLADGEFYRVGGHSPVKVDVRIIA
                     ATHQDLEVRVRDGSFREDLFHRLNVISVHVPTLAERRTDIPALASHFLEQAAEELGGE
                     PKIIRPDTMEYMMNLSWPGNVRQLENTCRWLTVMASGREIYLADLPPELGSDVVEQDT
                     NDSWEQALEAWADDLLREPGGLPLLDVAMPRFESVVIRAALRRTGGRKREAAELLGWG
                     RNTLTRKIAELGMTEL"
     gene            5761..6228
                     /gene="trmL"
                     /locus_tag="AC16VR_00525"
     CDS             5761..6228
                     /gene="trmL"
                     /locus_tag="AC16VR_00525"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HIR6009661.1"
                     /translation="MPLHVVLYQPEIPPNTGNVIRVCANTGARLHLVEPLGFEMDDSR
                     LRRAGLDYHEFACVDVHASLDDALDACGGGRCYAVSTRGSRRFSEITFTDGDVLLFGP
                     ETRGLPADVLETFPAAHVLRIPMRAGSRSLNLSNAVAVVVYEAWRQLGYAEGT"
     gene            complement(6230..6712)
                     /gene="secB"
                     /locus_tag="AC16VR_00530"
     CDS             complement(6230..6712)
                     /gene="secB"
                     /locus_tag="AC16VR_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HIR6009662.1"
                     /translation="MAESEPAGGEGVARGQIRLERLYLKDASFESPRSPAVFGESWKP
                     EMQLDINTRTNPQEGDRFEVVLTVTLRAKLDDGRTAYIIEVQQAGLFVIQGAEDAVVH
                     RVLGTMCPNTLFPYVRETLDSLAVRGGFPAIQLAPVNFDALYEEALKQRAPPANPVQH
                     "
     gene            complement(6676..>7036)
                     /locus_tag="AC16VR_00535"
     CDS             complement(6676..>7036)
                     /locus_tag="AC16VR_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR6009663.1"
                     /translation="VLLVFFVRNETQRGGRSVSAQELVDLVNREDAVVLDVRDKKDFD
                     QGHIPHALNIPYASLDGRIAELNAHKEKPIVIACKMGQHAGAAGTALRKQGFANVTRL
                     KGGIAEWRNQNLPVVKA"
BASE COUNT         1395 a         2318 c         2153 g         1170 t
ORIGIN      
        1 gatcgcgaga ctacgagctg gacgacatca tgctgctgaa ggtcggccgc cacctgcggc
       61 cgcggcccca tttcaagttg atcatcggcc gcgagctcgg cgagaaccgg tttctgcagg
      121 gctaccgcca ccagttcacg agcctgaccg ccatcagcca caaggggccg ctggccctca
      181 tcgacggaga cgccggcgcc gatgacatcg agctggcagc acgtatcctg gcccgatacg
      241 gtcaaggcag agatgcagcc agggttgagg tgatcgccgc ggagccaggc ggcgcggaaa
      301 cccgcctgag cgttgcgccg ctcgccgagg ccgatattgg gaccgattgg catgtcggag
      361 cttgaaacat tggatgctcg ggggttactg tgccccctgc cggtgataag aacccaggaa
      421 aaggtcaaag ggctcccacc gggcaccgtc gtcgagatcg ttgccaccga cccaggcgtg
      481 atgcaggaca ttccggcctg gtgccgggtc cacgggcacg agctcatctc gatgacggaa
      541 ggtgaagaag aagcggccgc cgaagggaag gccgacgcag acagattgat ccggggagga
      601 cgtgaaataa gagtcaggat caaagtgcgc cagtaatgct gatagtgcac caaaatggtg
      661 cgtaagcacc actttctcgc atccattcgc actatttctg agcgcctgtg agccggcact
      721 aacctctgat atcaagaaaa tggccgtggc acgcttactg cattccgcgg tcgcacggcg
      781 cggataccgc aaaagacatt actacgcaca ccctagaaag gagcacgtac acacaatggc
      841 cgaaaatact ctgaaactca tcaaggacaa cgacgtcaag tgggtggatc tgcgcttcac
      901 cgacccgaag ggaaaggaac agcacaccac cataccggcg caccggctcg acgccgacgc
      961 gctcaatgac ggtttcatgt tcgacggctc gtccatctcc ggctggaagt ccatcaacga
     1021 gtcggacatg atcctcatgc ccgacgactc cactcccgtc atcgacccgt tccgggacga
     1081 cacgaccctg aacctgcgct gcgacatcat cgagccctcc accatgcagc cctacgaccg
     1141 ggatccgcgc tctctggcca agcgggcgga ggcttacctg acttccaccg gaatcggcga
     1201 taccgcctac ttcgggcccg agaacgagtt tttcgtgttc gacgacgtgc gctggaaggt
     1261 ggaaatgagc ggcgcgtcct acagcatcgg cgctgaggag gcagcctggg aatcccattc
     1321 cgagttcgaa ggtggcaatc tgggccaccg cccgtccgtg aagggcggct acttccccgt
     1381 gcccccggtg gattccgccg ccgatctgcg ctcggccatg tgctcggcga tgatggccat
     1441 gggcctggac gtcgaggtgc accaccacga agtggcaacc gcctgtcaga acgagatcgg
     1501 cgcacgcttc aacactctgg tgcgtaaagc cgacgaggtg cagatataca aatattgcgt
     1561 gcacaacgtg gcggacgctt acggcaagac ggcgaccttc atgcccaagc cgctcgttgg
     1621 cgacaacggc aacggcatgc acgttcacca gtccatcgcc aagggggaca ccaacgtatt
     1681 ccacggcgac gagtacgcgg ggctctcaaa gacggctctg tactacatcg gcgggatcat
     1741 caagcatgca agggccatca acgcgtttgc gaacgccagc accaactcct acaagcggct
     1801 ggtgccaggc ttcgaggccc ccgtgctcct ggcttactcc gcccgcaacc gatccgcatc
     1861 gatccgggtt ccctacattc agggcggaaa cccccgggcc tatcgggtcg aagtccgctt
     1921 cccggacagc accggaaacc cgtacctgac cttcgccgcc atgctcatgg ccgggctcga
     1981 cggcatccag aacgagatcc atcctggcga ctcgttcgac aaggatctct atgacctgcc
     2041 cgccgaagag ctggagggcg tgccgaccgt ctgctcttct ttggagcagg cgctgcaggc
     2101 tctcgacgag gaccgcgact tcctcaaggc cggcggcgtg ttctccgacg gcatgatcga
     2161 cggctatctc gccctcaagc agcaggaagt ggactacctg aattccacga cccaccccgc
     2221 tgagttcgag atgtattaca gcgtgtaaga cgccctcgcg cgggtttcgc ccgcggcaaa
     2281 gccccaactc gtttacgaat tggggctttt ttttgcctcg gcgtggtcca aaatggctga
     2341 atccaaccca aggcgggcaa catgcagcta ctggcaacac tgaaaagaat tgcatttctg
     2401 agcatcatat ccacgccctg gctgatgctg agcacgcagt ccatggccgg aacgacgatc
     2461 tacaaaaccg tcgacgagga cggcaacgtg gtgttcacgg acacgccgcc cagagccgat
     2521 gggccttccg agacgatcac cctgacatcc cccaactctt tcgatcccgc agaagcaggc
     2581 accgagggtt ccggccgcca gctctggata gttgatccag acgccgaagg ggaagcctct
     2641 ggcgagttct acggggcatt gacggtcact tccccagcag acgacgagag catccgggaa
     2701 aacgcaggaa acgtgacggt tacggtgaag ctggagccgc cgaacctgca gcctggtcac
     2761 cgaatacgcc tcctgctcga tggctccccc acgggcggca ccaacacgac cgggcgtttc
     2821 gccctcagca acgtggaccg cggtacgcac tcgctgaagg cagaggtcgt ggacgacgcc
     2881 ggaaagctga tctacgcggg cccgtcgtcc accttccacc tgcagcgcta ttcggttctc
     2941 aacgcgccca agcgcccggt gaacccgatc aacaactgac ccgacaccgc agggcccatt
     3001 cgaccacgcc ggatcgcgag ggcggccgtc aaccggccgc accacaatcg acctttgcac
     3061 cgtattggtg catactgcct ggggcacggc gccgctaacg caagggggtg cccgccctga
     3121 tcggcccttt attccggcaa tgagaccgaa gtaaatactc actcacgtct tgcagcctga
     3181 tcgcgaggag ttggaccgtt tcttgcgtca ggagcacgca ttccgtcaac gccccatgac
     3241 tcagacacac gggaacactg cctacaacat gccgtctctg gccaaatccg acatcctgga
     3301 ccagctaacg ggcgcggtga tcgtctgcga tggctctctg agggtctgct acatgaaccc
     3361 cgccgcggag tctctgctga acacgagcga gcatcagagc accggggtgc agctcgcgga
     3421 actgctcttc gacgccgccg cttcgccgtt cgcggatctg gcgggcatct tcaccacaga
     3481 gcagtccatc accaaaagag cggcgcaatt ccagctgcgc gacggcaccg aggtgattgc
     3541 cgacctgacc gcctccctgg agccgaactc cggtcacctg gtcatcgagc tgcagccctt
     3601 gaaccgcctg cttcggatca accgcgacga gcactcgttc ttctcccagc agactacccg
     3661 tactctgatc agggggctcg ctcacgaggt gaagaacccg ctcggcggcc tgcggggcgc
     3721 tgcccagctg ctccagcgcg agctcgacgc cagcgagctg accgagtaca cgcgcatcat
     3781 catcgaggag gcggaccgcc tcacccagct cgtggacaac atgctgggat cgaaccgtca
     3841 gcccgagtac gcggacgtga acattcacga gcttctcgag cacgtggtgc ggctggtgga
     3901 tgcggagctc ggcggccggg tcaaatttgc cagggactac gaccccagcc tgccgccgat
     3961 tcgggccgat cgcgcgcagc tcgtccaggc gctgatcaac attgtcagaa acgccggcca
     4021 ggccctgggt gagacggcca acccctccat cacgctgaga accagggcag tcaggcagtt
     4081 caccatcggc tctacccggc atcgggtcgt gttgcagatc gagatagccg acaacggacc
     4141 cggaataccc gaaggcatgg tggaccagat ctggttcccg atgatcagcg ggcgagccga
     4201 cggcaccggt ctggggctgg ccattaccca atccatcgtt ggccatcaca acgggatcat
     4261 cgagtgcgtc agccgccccg gggacacccg cttttcggta ttcctgcccg tcgagagcaa
     4321 cggcgagcac atcgaacaac tggagaattc gcagccgtga gaaatcgagt ctgggtcgtc
     4381 gacgacgacc ggtccatacg ctgggtgctg gagcgggcca tgcagcaggc tgacattccc
     4441 attgccgctt tcgcctcggc cgatcacgcc atgcatcacc tgcaggacga gcggcccgtc
     4501 gccgtcctta ccgacgtgcg aatgcccggc atggacggca tggagttcat tggtcgcatc
     4561 cacgaggcct acccggagct gccggtcatc atcatgacgg cccattccga cctcgacagc
     4621 gccgtcaacg cctaccagag cggcgcgttc gagtatctgc ccaaaccgtt cgacgtggac
     4681 gaagccgtcg ccatcgtcaa acgggccatg acctttgccc aggaagaagc gccgccgccg
     4741 gcgcccgaac aggaaaccgc ccaggaaatc atcggccagg cgcccgccat gcaggaggtc
     4801 ttccgcgcca tcggccggct ggcacactcc aacgtcacgg tcctgatacg cggcgcatcg
     4861 gggtcgggaa aggagctggt cgcccgtgcg ctgcaccgcc acagcccccg cgcggagggc
     4921 gaattcgtcg ctttgaacat ggcggccata ccgagggagc tggtggaatc agagctgttc
     4981 ggccacgaaa agggcgcttt cacgggcgcc aactcgcgac gcgaagggcg tttcgagcag
     5041 gccgacggcg gcaccctgtt cctggacgag atcggcgaca tgcccgcaga aacccagacc
     5101 cggctgctgc gcgtgctggc cgacggggag ttctatcgag tgggcggaca ctccccggtc
     5161 aaggtggacg ttcgcatcat cgcagcaacc caccaggacc tcgaagttcg ggtgagggac
     5221 ggcagcttcc gggaggatct ctttcaccgg ctgaacgtca tcagcgtcca cgtgccgact
     5281 ctggcggaac ggcgaacgga cattcccgcg ctggccagcc atttcctgga gcaggcggcg
     5341 gaggaactgg gcggcgagcc caagataatc cgtcccgaca cgatggagta catgatgaat
     5401 ctgagctggc caggcaacgt gcgccagctc gagaacacct gccgctggct caccgtgatg
     5461 gcctccggac gtgaaatcta tctagccgac ctgccgccgg agctcggctc cgatgtggtc
     5521 gagcaggaca caaacgacag ctgggaacag gcgctggaag cctgggccga cgacctgctg
     5581 cgcgagcccg gcgggctgcc gctgctggat gtggcgatgc cgcgtttcga aagcgtggtc
     5641 atccgagccg cgctcaggcg caccggcggg cgcaagcgcg aggcggccga gctgctcggc
     5701 tggggacgca acacgctcac ccggaagatt gccgagctcg ggatgaccga actctgagcc
     5761 atgccccttc acgtcgtgct gtaccagccg gagattccgc caaacaccgg caacgtcatc
     5821 cgcgtctgcg cgaacaccgg cgcccgtctg cacctggtcg agccgctcgg tttcgagatg
     5881 gacgactcaa ggctccggcg ggcggggctc gactatcacg agtttgcttg cgtcgacgtt
     5941 cacgccagcc tggacgacgc gctcgacgcc tgcgggggcg gacggtgcta cgcggtgagc
     6001 acgcgcggtt ccagacgctt ctcggagatc acgttcaccg acggcgacgt gctgctcttc
     6061 ggtccggaga cccgggggct gccggcggac gtgctcgaga cgtttccagc ggcgcacgtg
     6121 ctgcgaattc ccatgcgggc gggctcgcgc agcctgaacc tctccaacgc cgtcgccgtt
     6181 gtggtctacg aggcgtggag acagctcggc tacgccgagg gaacttagat cagtgctgga
     6241 cggggtttgc cggaggtgcc cgctgcttga gcgcttcctc gtaaagcgca tcgaagttca
     6301 cgggcgcgag ctgaatggcg ggaaacccgc cgcgaaccgc cagcgagtcc agagtttccc
     6361 gcacgtaggg aaacagggtg ttcggacaca tcgttcccag cacccggtgc accacggcgt
     6421 cttcagcgcc ctgaatcacg aacaacccgg cctgctggac ttcgatgatg taggcggtgc
     6481 gcccatcatc gagcttggcg cgcagggtca cggtcaatac cacctcgaaa cgatcccctt
     6541 cctggggatt ggttcgggtg ttgatgtcca gctgcatctc gggcttccag ctttcgccga
     6601 acaccgccgg cgagcgcggc gattcgaacg aagcgtcctt gaggtacagg cgctccagac
     6661 gaatctggcc ccgggctacg ccttcaccac cggcaggttc tgattccgcc attcggcgat
     6721 gcctcctttg agccgtgtga cgttcgcgaa cccctgcttg cgcagcgccg tgcccgcagc
     6781 accggcatgc tgacccatct tgcaggcgat cacgatcggc ttttccttgt gagcgttcag
     6841 ctcggcgatt cggccgtcca gggacgcgta ggggatgttg agcgcgtggg gaatgtggcc
     6901 ctggtcgaaa tccttcttgt cccgcacgtc gagcaccacg gcgtcctccc ggttgaccag
     6961 gtcgaccagc tcctgagccg agacggaccg gccgccgcgc tgggtctcgt tgcgcacgaa
     7021 gaacaccagc agcacc
//
LOCUS       DBNHQR010000023         5545 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_508829, whole genome shotgun sequence.
ACCESSION   DBNHQR010000023 DBNHQR010000000
VERSION     DBNHQR010000023.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5545)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5545)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5545
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_508829"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..750)
                     /locus_tag="AC16VR_00540"
     CDS             complement(148..750)
                     /locus_tag="AC16VR_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009664.1"
                     /translation="MRAILVTVGLLLVAGCATQTTVVRADFTQGEGAYVVGYSGDPVI
                     RTRMEEQLVNDLRAREMIAFASHTDIPDITASSRYELIAAARKRGVISVIVINQVSGD
                     ASDSVVQNPERISPTHPDLQAFYDYSLGRQPVVEPGQQVFAEVNLFVLDGDEASLFWS
                     GTTWSFQADGQGTAIRGISETIADQLQKVRDQARANPFTQ"
     gene            complement(767..3814)
                     /locus_tag="AC16VR_00545"
     CDS             complement(767..3814)
                     /locus_tag="AC16VR_00545"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6009665.1"
                     /translation="MNIGSYSVENKVISWLLVIILVAGGIYGFNEMGKLEDPNFTIKQ
                     AKIITLYPGATAQEVQDEVTYHIEEALQLMGQLKRIKMSISRPGMSDLAIEFKDEYKA
                     DDFPDIYDELRRKLQDMHGKLPPGVQGPRVIDDFGDVYGIYAALTGDGYTYRDLKDAA
                     DELKKQLVLVPGVRKIVIGGGQREVVYIEISRTRISELGIPLEQIANILQSQNMVTDA
                     GRVRVGPDYVRIHPTGEFTSVQAIGDVLIGSNERRLIFLKDIATIHRAYEEVANKLIF
                     FNGKPALTLGVSIQAGENVVAVGEAVRKRFGQLASTIPIGMDIKVIYNQPEEVQKSVS
                     GFIVSVGQAIAIVIAVLLVFMGLRTGIIIGAVLLITVSGTLLFMQLYGIELQRISLGA
                     LVIALGMLVDNAIVVAEGMLVRIQAGMNPTKAAEETVGKTMWPLLGGTIIGILAFSAI
                     GLSTDNTGEFASSLFYVILISLTLSWVTAVSTTPLLCAMFLKPDPDADPDANPYDKGF
                     FRTFRGALKVVIRLRVLAVAVVVGLFVLAGFGFGYIKNAFFPNSNTPMFFVDIWEPEG
                     TDIRVTRRDAQAISEFLRAQEGVRSTVALIGGGDARFALTYEPKESSPAYAQIIVQTE
                     TLEQISKIWPAVEAHLQEHYPHLDPIIKAFRIGPGRDGKIEARFHGPDPAVLRSLAER
                     AKAIMREDPGAKEINDDWRQPVMLVRPVFNERVARQLGVTRENVATALKSAYEGTDVG
                     VYRDGIRMLPIKLRPPAEERADVANIQDIRVWSPALQTAVPIAQVVNSFETVFENTIL
                     RGRNRIQTIIAACNPTGELATPLFERLKPRIEDIELPPGYELSWGGEYEDSANAQAGL
                     ARVLPAGFILMILTSILLFGKVRQPLIIWLTVPLAIIGITAGLLGFDGAFDFMSLLGA
                     LSLIGLLIKNAIVLIEEIDQQIADGKEHLTAILDSTVSRLRPVVMAASTTILGLIPLL
                     QDVFFVNMSITIMAGLGFATFLTLLFVPVLYSLFFKVAYREDA"
     gene            complement(3848..4969)
                     /locus_tag="AC16VR_00550"
     CDS             complement(3848..4969)
                     /locus_tag="AC16VR_00550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6009666.1"
                     /translation="MNHRNIVAAIGCLALVACGEAPIDDAAPTARPVKTMIVESPAGS
                     GVRNFPGRIDSANRADLAFRVPGTVARLAASEGAPVNRGQELARLDQTDYEIALRDRQ
                     ATWDRASKDYERSKDLVDKGAISRRDFDRVEANFKSADAALAQAKQNLEYTTLRAPFD
                     GNVARRHVDAFEEINAGQTVYSVIDQSVLEVKIDIPENVIMMLSSRGSEPEEQAGKIP
                     VWAAFDMAPERRFPLTVKEYATRADAQTQTFEVTFALPAPDDVRVLPGMTASVTMDLS
                     RAMDVDSVYYLPVTAVTGSEGLTPRVWAVDEDTMTVREQPIVLGRLVGSSVEVTEGLE
                     PGMRIVTAGAAYLADGMAVTLLAHSEQAEPREEDLPRPS"
     gene            complement(5064..>5545)
                     /locus_tag="AC16VR_00555"
     CDS             complement(5064..>5545)
                     /locus_tag="AC16VR_00555"
                     /inference="COORDINATES: protein motif:HMM:NF016300.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HIR6009667.1"
                     /translation="VLSLTRSLKIEGEDVTAAVVNIQPRYRRGGGQNVIDGVTASRTI
                     AVVLRDLDRYGQLMNGALEVGVNSVSGTQLDSSDRAAEEEQALERAMQDARAAAARVA
                     DGFGVSVGRLLDVHVGQPPVQPMRGVMMEAAAAGDDFSPGQLVISRQLQATFAIETR"
BASE COUNT          927 a         1763 c         1825 g         1030 t
ORIGIN      
        1 tgccggagga gcgcgacggc gtggcatgga gcgtctccat caacaacccg gatggcgtga
       61 tgaccatcat ggtggcgggt gaaggcctcg gcgtggtcgg actgggaagc tgcgtcccgc
      121 ggtagacgcc gagaccacgg gtccgcctca ctgggtgaag ggatttgccc gtgcctgatc
      181 ccgcaccttc tgcagctgat cggcgatggt ttcgctgatg ccgcgaatgg cggtgccctg
      241 accgtcggcc tggaaagacc aggtggtccc gctccagaac aggctggcct catccccgtc
      301 caggacgaac aggttgacct cggcaaaaac ctgctggccc ggctcgacga ccggctgccg
      361 gccaagggaa tagtcgtaga atgcctgcag gtccggatgc gtgggtgaga tccgctcggg
      421 gttctggacg acgctgtcgg aagcgtcgcc tgagacctgg ttgatcacga tgacgctgat
      481 cacaccacgc ttgcgggctg ccgcgatgag ctcgtagcgg ctgctcgcgg taatgtccgg
      541 aatgtcggtg tggctggcaa acgcgatcat ctcccgtgcc ctgagatcgt tgaccagctg
      601 ctcttccatg cgcgtccgga tcaccggatc cccgctgtac cccacgacgt aggcgccctc
      661 gccctgggtg aaatcggccc gaaccaccgt ggtctgggtc gcgcagccgg ccaccagaag
      721 cagccccacg gtgaccagaa ttgcgcgcat gtttcctcca gcgatctcaa gcgtcttcac
      781 ggtaagcgac cttgaagaac agcgagtaca gcacgggtac gaagagcagt gtcaggaacg
      841 tcgcgaaccc gagcccggcc atgatggtga tggacatgtt gacgaagaaa acgtcctgca
      901 ggagcgggat caggccgagg atggtcgtgg acgccgccat caccaccgga cgcagacggc
      961 tcaccgtgga gtccaggatg gcggtcaggt gctctttgcc gtctgcgatc tgctggtcga
     1021 tctcctcgat cagcacgatg gcgttcttga tgaggagccc gatgaggctc agcgcgccga
     1081 gcagtgacat gaagtcgaac gcgccgtcga aaccgaggag ccctgcggtg atgccgatga
     1141 tggcgagcgg caccgtgagc cagatgatca gcggctggcg gactttaccg aacagcagga
     1201 tgctggtgag gatcatgagg atgaagcccg cgggcagcac ccgggcgagg ccggcctggg
     1261 cgttggccga gtcctcgtac tcgccgcccc aggacagctc gtagcccggc ggcagctcga
     1321 tgtcttcgat ccggggcttg agccgctcga acagcggcgt ggccagctcc cccgtggggt
     1381 tgcaggcggc gatgatggtc tggatgcggt ttcggccgcg cagaatcgtg ttctcgaaca
     1441 cggtctcgaa cgagttcact acctgagcga tgggaaccgc cgtctgcagc gccgggctcc
     1501 atacccggat gtcctggatg ttcgccacgt cggcgcgttc ttccgccggc gggcgaagct
     1561 tgatcggcag catgcggatg ccgtcccggt agacgcccac gtccgtccct tcgtaggcgg
     1621 acttgagcgc ggtggcgacg ttctcccgcg tgacgccgag ctgcctcgcg acccgctcgt
     1681 tgaacaccgg gcgcaccagc atgacgggct gccgccagtc gtcgttgatc tccttggccc
     1741 ccggatcctc gcgcatgatg gctttcgccc gctcggccag ggagcgcaac accgcaggat
     1801 ccgggccatg gaatcgcgcc tcgatcttgc cgtcgcgccc cgggccgatt cgaaaggcct
     1861 tgatgatggg atccaggtgc gggtagtgct cctgcaagtg ggcctctacc gccggccaga
     1921 ttttcgagat ctgctccagg gtctcggtct gcacgatgat ctgggcgtag gcggggctgg
     1981 attccttggg ctcgtaggtc agcgcaaacc gggcgtcgcc gccgccgatc agggcgaccg
     2041 tggatcgcac gccttcctgg gctctcaaga attccgagat tgcctgggcg tcgcgtcggg
     2101 tgacgcgtat gtcggtgccc tcgggttccc agatgtcgac gaagaacatg ggcgtgttcg
     2161 agttcgggaa gaaggcgttc ttgatgtacc cgaagccgaa acccgccagc acgaacagac
     2221 cgaccaccac ggccaccgcg agcacccgca ggcggatgac caccttcagc gcgccgcgga
     2281 aggttctgaa gaaacccttg tcataggggt tcgcatcagg atcggcatcg ggatccggct
     2341 tcaagaacat ggcgcacagg aggggcgtcg tgctgaccgc cgtcacccag gacagcgtca
     2401 gggagatcag gatcacgtag aacagggagc ttgcgaactc gcccgtgttg tctgtcgaca
     2461 ggccgatggc ggaaaacgcg aggatgccga tgatggtgcc gccgagcagc ggccacatgg
     2521 tcttgccgac cgtttcctcg gcggccttgg tggggttcat gccggcctgg atgcgaacga
     2581 gcatgccttc cgctaccacg atggcgttgt ccaccagcat gcccagcgcg atcaccaggg
     2641 cgcccaggga aatccgctgc agctcgatgc cgtagagctg catgaagagc aacgtgccgg
     2701 agacggtgat cagcagcacg gcgccgatga tgatgccggt gcgcagcccc atgaagacca
     2761 gcagcacggc gatcacgatg gcgatcgcct gcccgacgct gacgatgaag ccggataccg
     2821 acttctgcac ttcctcgggc tggttgtaga tgactttgat atccatgccg atgggtatcg
     2881 tgctcgccag ctgcccgaat cgctttctca cggcctcgcc cacggcgacc acgttctcgc
     2941 cggcctggat ggacacgccc agggtcagcg ccggcttacc gttgaagaag atgagcttgt
     3001 ttgcaacctc ctcgtaggcc cggtgaatgg tcgcgatgtc tttgaggaaa atcagacgac
     3061 gctcgttcga accgatgagc acgtcgccaa tggcctggac ggacgtgaac tcgccggtgg
     3121 gatgaatgcg gacgtagtcg ggtccgaccc gaacccgtcc cgcgtccgtg accatgttct
     3181 gggactgcag gatgttggcg atctgctcca gcggtatgcc gagctcgctg atccgcgttc
     3241 gtgagatctc gatgtagacc acctcgcgct ggccgccgcc gatgacgatc ttcctcacgc
     3301 cgggtaccag cacgagctgc ttcttgagct cgtcggcggc gtccttcagg tcacgatacg
     3361 tatagccgtc gccggtcagc gccgcgtaga tgccgtagac gtcgccgaag tcgtcgatga
     3421 cgcgtggccc ctgcacgccg ggcggcagct tgccgtgcat gtcctgcagc ttgcggcgca
     3481 gctcgtcgta aatgtccggg aagtcgtcgg ccttgtactc gtccttgaat tcgatggcga
     3541 gatcggacat gcccggtcgg gagatggaca tcttgatgcg cttgagctgg cccataagct
     3601 gcagcgcttc ctcgatgtgg taggtcacct cgtcctgcac ttcctgagcc gtagccccgg
     3661 gatacagggt gatgatcttc gcctgcttga tggtgaagtt cggatcctcc agcttcccca
     3721 tctcgttgaa gccgtagatt ccgccggcca ccagaatgat gaccagcagc caggagatga
     3781 ccttgttctc gacggagtat gagccgatgt tcatcgttgt tgttgtccgt tagcgccgcg
     3841 cgagccgtca gctcgggcgc ggcaggtctt cttcccgcgg ctcggcctgc tcgctgtggg
     3901 ccagcagcgt taccgccatg ccatccgcca ggtaggcggc tccggcggta acgatgcgca
     3961 tgccgggctc caggccctcg gtgacctcga cgctggaccc caccaggcgc cccagcacga
     4021 tcggctgctc gcgcacggtc atggtgtctt catccacggc ccagactctt ggggtcaagc
     4081 cctcgctgcc ggttacggcc gtgacgggaa ggtagtagac ggagtccacg tccatggccc
     4141 gggacagatc catggtgacg ctggccgtca tcccgggcag aaccctgacg tcgtcaggtg
     4201 ccggcagggc gaacgtcacc tcgaaggtct gtgtctgagc gtcggcgcgt gtggcgtact
     4261 cctttaccgt cagaggaaag cggcgctccg gggccatgtc gaaggcggcc cataccggga
     4321 ttttgccggc ctgttcctcg ggctcggacc cgcgcgatga cagcatcatg atgacgtttt
     4381 ccgggatgtc gattttcact tccagcacgg attggtcgat cacggagtag acggtctggc
     4441 cggcgttgat ctcctcgaag gcatccacat gccgtctggc cacgttgccg tcgaagggtg
     4501 cgcgcagggt ggtgtactcg aggttctgct ttgcctgggc cagcgccgca tcggcgctct
     4561 tgaagttggc ctccacccgg tcgaagtcgc gccgggagat ggcccctttg tccaccagat
     4621 ccttggagcg ctcgtagtcc ttgctggccc ggtcccaggt ggcctggcgg tcacgcagcg
     4681 cgatctcgta gtcggtctgg tccaggcgcg cgagctcctg tccccgattg acgggcgcgc
     4741 cttccgaggc cgcgagcctg gctaccgtgc ccggtacgcg aaaggccagg tccgccctgt
     4801 tcgccgaatc gattcggccg gggaagttgc gaacgccgct gccggcggga gactcgacga
     4861 tcatcgtctt caccggccgc gctgtggggg ccgcgtcgtc gatgggcgct tcgccgcacg
     4921 cgaccagggc caggcagccg atggcggcca ctatattcct gtggttcaag atccggtcct
     4981 ccactttccc ggggctatac tgcccgcatt cggagccgtt ctccagagag gtccatacgg
     5041 agtctttggc cgccgcgcag aagtcaacgg gtttcgatgg cgaatgtggc ctgcagctgg
     5101 cgggagatga ccagctgtcc cgggctgaag tcgtctccgg ctgccgccgc ctccatcatc
     5161 acgccgcgca tgggctggac gggaggctgg cccacgtgca catccagcag cctgcccacg
     5221 gagacgccaa aaccgtcggc gacgcgagca gccgcggcgc gcgcgtcctg catcgcccgc
     5281 tccagggctt gctcttcctc tgccgcccgg tcgctggagt cgagctgggt accgcttacc
     5341 gagttcaccc ccacttccag agcgccgttc atgagctggc cgtaacggtc gagatcgcgc
     5401 aataccaccg cgatggtgcg ggatgcggtg accccgtcga tgacgttctg gccgccccca
     5461 cggcggtagc gaggttggat gttgaccacc gccgcggtca cgtcctcacc ctcgatcttc
     5521 agagagcgcg tcagggacag cacct
//
LOCUS       DBNHQR010000024         3477 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1015651, whole genome shotgun sequence.
ACCESSION   DBNHQR010000024 DBNHQR010000000
VERSION     DBNHQR010000024.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1015651"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..1247)
                     /gene="dgcN"
                     /locus_tag="AC16VR_00560"
     CDS             complement(165..1247)
                     /gene="dgcN"
                     /locus_tag="AC16VR_00560"
                     /inference="COORDINATES: protein motif:HMM:NF041892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase DgcN"
                     /protein_id="HIR6009668.1"
                     /translation="MIRDDGDLAEVPRLRSPNDMASAGTRIEAPYLIFLGSAHDQLAA
                     KTGQGIVDWRPQNCIGQLRLPECQADLGLPDLSVAEAAAAGARTLVIGTVSPGGSLPE
                     KWLPILHAALANGLDIASGMHDRLDSFPEIRDAAARHDRRLVDVRHSTVRFPTGSGRK
                     RSGRRLLTVGTDCSVGKKYAALALEREMRSRGMDAEFRATGQTGILIAGSGVAIDAVT
                     ADFISGAAECLTPDAERGHWDVVEGQGSIFHPSFAGVSLGLLHGTQPDAVVVCHEPTR
                     RTMRNVSTLMPRIGEVIEQTLVHGRTTNPDVVCIGVAVNTAALDEGEARELLTNYAAQ
                     LGLPVTDPIRFGAGLLVDELVKNFPD"
     gene            complement(1244..2260)
                     /locus_tag="AC16VR_00565"
     CDS             complement(1244..2260)
                     /locus_tag="AC16VR_00565"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6009669.1"
                     /translation="MDTAEPGSQAWLDLVEEPIIDPDRVIVDPHHHLWDQPSAFGRYT
                     LEDLWRDTGSGHRIAKTVFLECGASYRESGPEHLRCVGETEYVTQAARASSAQSDGNA
                     VIAGIVARADLRLGDALDEVLDAHVEVAGDLFKGIRHSGAHAREPEILRIAGRAPAQL
                     YANDAFRRGVRRLGELRLTYDTWHYHYQNPQFLALARAVPDTIMVLDHFGTPLGVGPY
                     ADRREEIFRQWQDDMSRLADCPNVVAKLGGLAMPDNGFGWHAAARPPTSEEFAEAQRP
                     YYRHMIDVFGADRCMFESNFPVDRMSLSYAVLWNGMKRIAADFSEAEQDALFSGTATR
                     VYRL"
     gene            complement(2392..>3477)
                     /locus_tag="AC16VR_00570"
     CDS             complement(2392..>3477)
                     /locus_tag="AC16VR_00570"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6009670.1"
                     /translation="DLRAELSRYVGVYDIGDSFRAGKQEIKLSLLPEARNLGLTLADL
                     ASQVRSAFYGYEAQRIQRGQDDVRVMVRFPEAERTSIGNLEDMYIRTPQGTEVPFYSV
                     AKFELGRGYSTIRRVNGRRVINVTAEVDRSVVSPEEIGSSISSKVLPKLRAKYPSLKV
                     GVSGEQEERTKAFTGLAQAAMLALIVIYALLAIPLRSYLQPLVIMSVIPFGAVGAIVG
                     HWLMGYQLMFFSALGMVALSGVVVNASLVLVDYTNRRRREGMPIAEALRSAGVVRFRP
                     ILLTSVTTFVGLIPLMFSDTPATAFFIPMAISLAFGVLFATAITLLLVPALYHLAEDV
                     SGWDPVAQGVEEHRSNIRSTGKLQPGG"
BASE COUNT          599 a         1109 c         1175 g          594 t
ORIGIN      
        1 agcggggata gggcagacat tcgcccatgc cggtcaggtc gccctcacgc acggtcacca
       61 cgaccacctc ggccaccgtc ttgctgcctc gggcgatggt aaaagtgccg gcgatgggcc
      121 aactctcgat gtgtgcgaaa acgtccatgg ggagccggcg gctttcagtc cggaaagttt
      181 ttgacaagct catccacgag caagcccgcg ccgaagcgga tgggatccgt gacgggcagc
      241 ccgagctgcg cggcgtagtt ggtcagcagc tcacgagcct cgccctcgtc gagtgcagcc
      301 gtgttcaccg cgacgccgat gcagacgacg tcggggttgg tggttcgacc atgcacgagc
      361 gtctgctcga tcacctcgcc gatgcgcggc atgagcgtgc tgacgtttcg catggtccgg
      421 cgggtcggct cgtggcagac cacgacggcg tccggctggg tcccgtgcag cagcccgagg
      481 gaaacgccgg cgaaagaagg gtgaaagatc gagccctgac cctcgaccac gtcccagtgg
      541 ccgcgttccg catccggggt caggcactcc gcagcgcccg agatgaagtc cgccgtgacg
      601 gcgtcgatcg cgacgccgct gccggcgatg agaatgcccg tctggcccgt ggccctgaac
      661 tcggcgtcca tgcccctgct gcgcatctcg cgctccaggg cgagcgccgc gtacttcttg
      721 cccactgagc agtcggtgcc gacagtgagc aaccgtcggc cgctacgttt tctgcccgac
      781 cccgtaggga aacggacggt gctgtgacgc acatcgacca gacgacgatc gtggcgcgcc
      841 gcagcatccc ggatctccgg aaagctgtcc aaccggtcgt gcattccgct cgcgatgtcg
      901 agaccgtttg ccagcgcggc gtgcaggatg ggcagccatt tctcgggcag cgaaccgccc
      961 gggctgaccg ttccgatcac gagggtgcgg gcgccggctg ccgccgcttc ggccacggac
     1021 aaatcgggta ggccgagatc cgcctggcac tcgggcaatc tcagctgccc gatgcagttc
     1081 tgcggccgcc agtcgacgat gccctggccc gttttcgcgg ccagctggtc gtgggcgctg
     1141 cccaggaaga tcaggtaagg cgcttcgatc ctcgtgccgg ctgacgccat gtcgttcgga
     1201 ctgcgcaatc tgggaacctc cgccaggtcg ccgtcgtctc tgatcaaagg cggtacacgc
     1261 gggtcgcggt gccgctgaac aacgcatcct gctccgcttc gctgaaatcg gcggcgatgc
     1321 gcttcatgcc gttccacagc accgcgtatg acagcgacat gcgatccacc ggaaaattgc
     1381 tctcgaacat gcaccggtcg gcgccgaaca cgtcgatcat gtgccggtag taggggcgct
     1441 gggcctcggc gaattcctcg gaggtcggcg gccgggcagc ggcgtgccaa ccgaaaccgt
     1501 tgtccggcat ggccagcccg cccagcttgg cgaccacgtt ggggcagtcg gctagccggg
     1561 acatgtcatc ctgccactgg cgaaagatct cttcgcgccg gtccgcgtaa ggaccgacgc
     1621 ccagcggggt gccgaaatgg tcgagcacca tgatggtgtc cggtacggcc cgtgccagcg
     1681 ccagaaactg ggggttctga tagtgatagt gccaggtgtc gtaagtcagg cggagctcgc
     1741 cgagccgtct gacgccgcgg cggaaggcat cgtttgcata cagctgcgcg ggggcccggc
     1801 cggcgattcg gaggatctcg ggctcccggg cgtgggcccc tgagtggcgg atgcctttga
     1861 acaggtctcc cgcgacctcg acgtgggcgt ccagcacctc gtccagggcg tcgccaagcc
     1921 gcagatcggc tcgggccacg atgccggcaa tcaccgcatt tccgtcgctt tgagcagagg
     1981 aggcgcgggc ggcctgcgtg acgtattccg tctcgccaac gcaacgcagg tgctcggggc
     2041 ccgattcccg gtaacttgct ccgcattcca gaaacacggt tttcgcgatg cggtggcctg
     2101 accccgtgtc gcgccacagg tcttccagcg tgtagcgtcc gaacgcgctt ggctggtccc
     2161 agaggtggtg atggggatcg acgatcacgc gatcgggatc gatgatcggt tcctcaacga
     2221 ggtccagcca ggcctggctg ccaggttcgg cggtatccaa ggcgtcgtct ccgagcggcc
     2281 attcaggagt cgtcatctta gtacaggacg gctcggtcat gctcgcgtct ggatggggcg
     2341 gtggggtttg gcttaccccg gagcgctgcc cgggtcgtca gggcgctcgc gtcagccgcc
     2401 cggctgcagt ttgcccgtcg agcggatatt tgagcgatgc tcttcaacgc cctgggcgac
     2461 ggggtcccag ccggagacgt cctcggccag gtggtaaagc gcgggcacta gcagcaacgt
     2521 gatggcggtc gcgaacagca cgccgaaagc cagcgagatg gccatgggga tgaagaatgc
     2581 ggttgccggg gtgtcgctga acatcaacgg gatgaggccg acgaacgtgg tcacggaggt
     2641 cagcaggatg ggcctgaatc ggaccacgcc ggcgctgcgc agggcttccg cgatcggcat
     2701 gccctcccga cgacggcgat tcgtgtagtc caccagcacg aggctggcgt tcacgaccac
     2761 cccggacaga gccaccatgc ccagcgccga gaagaacatc agctgatagc ccatgagcca
     2821 gtgaccgacg atggcaccaa cggctccgaa gggaatgacg ctcatgatga ccagcggctg
     2881 caggtaggat cggaggggaa ttgccagcag cgcgtagatg acgatcagag cgagcatggc
     2941 ggcctgggcc agtcccgtga acgctttggt cctctcttcc tgctctccgg agacgccgac
     3001 cttcaaagag gggtacttgg cgcgaagctt cggcagcacc ttggatgaga tggacgaacc
     3061 gatctcctcg ggcgaaacga cgcttcggtc cacctcggcg gtgacgttga tcaccctgcg
     3121 cccgttcacg cggcggatcg tagagtaacc gcgacccagc tcgaacttgg ccacgctgta
     3181 gaagggtacc tcggtgccct ggggcgtgcg gatgtacatg tcttccaggt tgcctatgga
     3241 cgttctctcg gcctcgggaa atcgcaccat gacgcgaacg tcgtcctggc cgcgctgaat
     3301 gcgctgggct tcgtagccgt agaacgcgct gcgcacctgg ctggccagat cggcaagggt
     3361 cagtcccaga ttgcgggcct cgggaagcag ggagagcttg atttcctgct tgccggcccg
     3421 gaacgaatcg ccaatgtcgt agacgcccac gtagcggctg agctcggcgc gaagatc
//
LOCUS       DBNHQR010000025         1962 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_826000, whole genome shotgun sequence.
ACCESSION   DBNHQR010000025 DBNHQR010000000
VERSION     DBNHQR010000025.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_826000"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..166)
                     /locus_tag="AC16VR_00575"
     CDS             complement(<1..166)
                     /locus_tag="AC16VR_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR6009671.1"
                     /translation="MTPLAQFEPGVRAWFTERFPSPTAVQAQSWPRIAAGEHVLITAP
                     TGSGKTLTAFL"
     gene            complement(163..1011)
                     /locus_tag="AC16VR_00580"
     CDS             complement(163..1011)
                     /locus_tag="AC16VR_00580"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF044908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR6009672.1"
                     /translation="MIHGNPADLVDLLSVHPTREEGYQAGQVREARSRVYGGQFLGQG
                     ICAASQIGHGALRSFHGYYLRPGATDLGIGYQVERLGDMVVGVSAVQREKQLFLMMAR
                     FGPFCPAPPEEMPDVPPPEDCIPRERGIAGLDRDTDSTWAVTDSPFDNRFVEDIWNED
                     YRRPLHHVWFRLRDPGALPGHIDQIVMQAIAAYYADDTIMDNALFPHGWHRCWSELQT
                     ASLDHAMWFHRPMDLRRWHLFAQDSPVASGGRAMTRGTIFDDRGAVVASAAQEILLRE
                     PPDDKP"
     gene            1081..>1962
                     /locus_tag="AC16VR_00585"
     CDS             1081..>1962
                     /locus_tag="AC16VR_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009673.1"
                     /translation="MKELSALLEPGQRIFVAGSSNEPTGLLEVLADQTLPPEIEFLQF
                     PLAGYNRFDFTAFSEDAIFTTFFMTPHLRDADPARVRFLPMQMRAVYDFLAEGVDVVL
                     LQVARNASGQLCVGPNVDFVEAAMMGAKTVAAELNHGFVAPAGCPVVDAGRIDFLMET
                     DRSLFEMAPPEIDGAAARIGEHVAQLVRDGDCIQTGIGSIPAAILARLGDRNDLGMHG
                     GLIDDGGMALIRNGNMTGVNKPIDTGKHIAGMALGSRQLLDWLAQTPAVQFRGANHTH
                     EVNVIRELPQFLSVNSAV"
BASE COUNT          345 a          606 c          641 g          370 t
ORIGIN      
        1 acagaaaagc ggtcagcgtt ttgccgctgc ccgtcggtgc cgtgatcagc acatgctccc
       61 ctgctgcgat ccgcggccag ctctgcgcct gaacggcagt gggggaagga aagcgctcgg
      121 tgaaccacgc acgtactccg ggttcgaact gcgccagagg tgtcacggct tgtcgtccgg
      181 tggttcgcgc agaaggatct cctgggcggc tgaggccacc accgcgcccc gatcgtcgaa
      241 tatggtccca cgggtcatgg cgcgcccgcc gcttgccacc gggctgtcct gggcaaagag
      301 gtgccagcgt ctgagatcca tgggtcggtg gaaccacatg gcgtggtcga ggctcgccgt
      361 ctggagttcg ctccagcatc ggtgccagcc gtgtggaaag agcgcgttgt ccatgatggt
      421 gtcgtccgcg tagtaggcgg caatcgcctg catcacgatc tgatcgatat ggccgggcag
      481 cgcgccggga tcgcgcaggc ggaaccagac gtggtgaagc ggtcgccggt agtcttcgtt
      541 ccagatgtcc tcgacgaacc ggttgtcgaa aggggagtct gttacggccc atgtggaatc
      601 ggtgtctcgg tccaggccgg caatgccgcg ctcccgtgga atgcagtcct cggggggcgg
      661 cacgtcgggc atctcctcgg gtggagcggg gcagaaaggg ccgaagcgcg ccatcatgag
      721 aaacagctgc ttctcgcgct gcacggcgga cacgccgaca accatgtcgc cgaggcgctc
      781 cacctggtag ccgatgccca ggtccgtcgc cccgggacgc agatagtagc cgtgaaagga
      841 cctcagggcg ccgtgcccga tctggctggc ggcacagatg ccctgaccga gaaactggcc
      901 tccgtatacc cgcgagcgtg cttcgcgcac ctgacccgct tgatagcctt cctctctcgt
      961 cgggtggact gaaagcaaat cgaccaggtc tgcggggttg ccgtgaatca tctgccgatt
     1021 attacgaatt gcccctgggc gaggctagaa tctcgccctt gcctccagga tggtgaaatc
     1081 atgaaagagt tatcggctct gctggagccg ggtcagagga tattcgtggc gggcagttcc
     1141 aacgagccga ccgggttgct cgaggtgctg gcggatcaga ctctgccgcc ggaaatcgag
     1201 ttcctccagt ttccgctggc tggatacaac cgcttcgact tcacggcctt ctccgaggac
     1261 gcgatcttca caaccttttt catgacgccg catcttcgcg atgccgatcc ggcgcgcgtg
     1321 cgtttccttc cgatgcagat gcgagccgtg tacgacttcc tggccgaagg tgtcgacgtc
     1381 gtgctcctcc aggttgcgcg caatgccagc ggccagctct gcgtaggccc caacgtggac
     1441 ttcgtggagg ccgcgatgat gggcgcgaag accgtggcgg ccgagctgaa tcacggcttc
     1501 gtcgcccccg cgggttgtcc ggtggtcgac gccggtcgga tcgactttct catggagacg
     1561 gaccgttcgc tgtttgagat ggctcctccg gagatagatg gggcggcggc gcgcatcggt
     1621 gagcacgtcg cgcaactcgt tcgcgacggc gactgcattc agaccggcat aggcagcatc
     1681 cctgccgcta tccttgcaag gctcggcgac aggaatgatc tcggcatgca cggcgggctt
     1741 atcgacgacg gcgggatggc gctcatccgt aatgggaaca tgaccggcgt taacaagccc
     1801 atagacacag gaaaacacat agccggcatg gcgctcggat ctcgccagct gctcgactgg
     1861 ctggctcaaa ccccggcggt acagttccgg ggagccaacc acactcacga ggtcaacgtg
     1921 atacgcgagc tgccgcagtt tctttctgtc aactccgccg tc
//
LOCUS       DBNHQR010000026         5978 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1523008, whole genome shotgun sequence.
ACCESSION   DBNHQR010000026 DBNHQR010000000
VERSION     DBNHQR010000026.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5978)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5978)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5978
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1523008"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..873)
                     /gene="galU"
                     /locus_tag="AC16VR_00590"
     CDS             complement(37..873)
                     /gene="galU"
                     /locus_tag="AC16VR_00590"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-alpha-D-glucose metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HIR6009674.1"
                     /translation="MIRKCLFPVAGYGTRFLPATKAMAKEILPVLNKPLVQYAVEEAL
                     EAGLTDVGFVTGRGKRAIEDHFDTSYELEHQIAGTGKEKALDGIRRIMEECTFSYTRQ
                     VNMRGLGDAILKGRTLVGENPFGVVLADDLCFGNGHREGGVLKQMVKLFKQFRCSIVA
                     IEEVPEDRVSSYGVIAGEALSDRLVRITDMVEKPSPENAPSNFGIIGRYILTPDIFEI
                     LENTEPGKNGEVQITDALLQQAKDGCVIAYRFQGKRFDCGSVAGFVDATNYCYEHLYT
                     ET"
     gene            complement(1008..1280)
                     /locus_tag="AC16VR_00595"
     CDS             complement(1008..1280)
                     /locus_tag="AC16VR_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009675.1"
                     /translation="MNDRFESLPLFPDEAPPAKRRQVDAGRSEPGRGRASAAARSLGS
                     RDPHLIAANRLARAIRRLPDHSPEQTRTGQAICRHLLALLEEAERR"
     gene            complement(1347..3914)
                     /gene="clpB"
                     /locus_tag="AC16VR_00600"
     CDS             complement(1347..3914)
                     /gene="clpB"
                     /locus_tag="AC16VR_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018405156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HIR6009676.1"
                     /translation="MRPDKMTSRLQNALADAQSMALGKDHAAIEPLHLLAVLVEQKAG
                     SVLPQLVRAGARANELQTRLNERLGQLPTLKSPTGEMSFAPAFVKVLNLADRLAQKAG
                     DDFISTEQVLLALFEDPTVESVLRESGVDKAALEAIVSQARSGETVSDPNAEEHREAL
                     EKYTVDLTAQAEEGKLDPVIGRDDEIRRTVQVLQRRTKNNPVLIGEPGVGKTAIVEGL
                     AQRILNGEVPEGLKNKHILALDMGSLIAGAKFRGEFEERLKAVLNELSKQEGNVILFI
                     DELHTMVGAGKAEGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQK
                     VLVDEPSVEDTIAILRGLKERYELHHAVDITDPAVVAAARLSHRYITDRQLPDKAIDL
                     IDEAASRIRMEMDSKPEAMDRLERRLIQLKMEAEALKQESDDASRKRLEDLQSSIAEL
                     EREYADLEEIWNAEKASLRGAQDLKEDLDAARGEFEVARRAGDLARMSELQYGTIPEL
                     EKQLAAASNGEAEPRQLLRNSVTEEEIGEVVSKWTGIPITKLLEGERDKLLRLEEALH
                     ERVVGQDEAVRIVADAVRRSRAGLSDPDRPNGSFMFLGPTGVGKTELCKALAQVLFDS
                     DEALVRVDMSEFMEKHSVARLIGAPPGYVGYEEGGFLTERIRRRPYSLILLDEIEKAH
                     PDVFNILLQVLDDGRLTDGHGRTVDFRNTVLVMTSNLGSEAIQSLTEAGNADRIKDTV
                     LGVVSQHFRPEFLNRVDDIVVFHPLGREHVAEIAKLQFDILRRRLEEQDLTIDIAPAA
                     VEALVEEGYDPVYGARPLKRVIQRRIENPLASRILGGDFLPGSTIRVGYADGDYSFD"
     gene            3981..4739
                     /locus_tag="AC16VR_00605"
     CDS             3981..4739
                     /locus_tag="AC16VR_00605"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR6009677.1"
                     /translation="MEQRKHPRVQLPLLVELHHPSLPQGKLRCVARDVSKGGVYVHFD
                     QPPTLIPGAKLRLTLLNPSQVESQPTPTVEMMVARTEPTGLGLAFVNATSQHLWASVE
                     RLRDELAIGRDYFQVFQGAVVLNEQDEVLLVQQHGKWGFPGEYLVVGEDAETALKAML
                     TRRFALADLTIDGVLKVGSGATIDLPEAAVFRVYHRVTTSTAEISASAEEAYRKVRWV
                     ENRRSVAQMAFVSKELRELANAALLWAAGRRRAG"
     gene            complement(4765..>5978)
                     /locus_tag="AC16VR_00610"
     CDS             complement(4765..>5978)
                     /locus_tag="AC16VR_00610"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIR6009678.1"
                     /translation="RSVREPRLAGVAAALVRAQYALLLGLSPLLVMLQGQIPSSGPVA
                     NLVAVPVISFLVIPAALIGALLMELVPFLGEAALGMAAVVVAQLMGVLEASASLPPLV
                     AAPRLLPVFVALATGLVMLACGGSGRHLLLCLAWAVWALPPDQGLPRGEFRVLAMDVG
                     QGSAVLVDTKRHRLLFDAGPRYPSGFDLGAQVVLPTLVATGSPRLDALVISHADIDHS
                     GGKGAVVGAVPVGEVYESRPDSESSRCSAGQSWRWDGVDFRFLHPPPLARARGLGSND
                     ASCVLLIDNGRERALLPGDIGAAVEGQLLRALDPVDVLLAAHHGSASSSSRAFVRVLE
                     PDVVFISAGRRNRYGHPHPDVVGRFAEVGARVHSTAEHGALVWDSRTPGSVRRWRRDT
                     PRYWVQGSQAD"
BASE COUNT         1074 a         1996 c         1850 g         1058 t
ORIGIN      
        1 gcagctttaa agcaacgtca aacgacatgc gcctggctag gtttccgtat agagatgctc
       61 gtagcagtag ttggtcgcgt cgacgaaccc ggccaccgaa ccgcagtcga agcgcttccc
      121 ctggaaccgg taggcgatga cgcagccgtc cttagcctgc tggagaagcg cgtccgtgat
      181 ctggacctca ccgttcttgc cgggctccgt gttctccaga atctcgaaaa tgtcaggggt
      241 gagtatgtag cgtccgatga tgccgaagtt gctgggcgca ttctcaggac tcggcttctc
      301 caccatgtcc gtgatgcgta ccaggcggtc gctaagcgct tccccggcga tgacgccata
      361 gctgctcacc cggtcctcgg gcacctcctc gatcgccacg atgctgcagc gaaactgctt
      421 gaagagtttc accatctgct tgaggacccc gccctcgcgg tggccgttgc cgaaacacag
      481 atcgtcggcc agcaccacgc cgaaggggtt ctcgccgacc agcgtgcgtc ctttcagaat
      541 cgcatccccc aggccccgca tgttgacctg ccgcgtgtac gagaacgtac actcctccat
      601 gatccggcgg ataccgtcca gggccttttc cttgccggtc ccggcgatct gatgttccag
      661 ctcatagctc gtgtcgaagt ggtcctcgat agcgcgcttg ccccgccccg tcacgaagcc
      721 gacgtcggtg agaccggcct ccagggcttc ctccactgcg tactgaacga gaggcttgtt
      781 gaggacggga aggatttcct tggccatcgc cttggtcgcc ggcagaaaac gggtcccgta
      841 accggcgacg ggaaacaaac acttcctgat catgaaatcc atctcaaaga atcagtacaa
      901 atgacgatgg taggacggtt agcgtccact accatggacg aaccgcacag attgaacaac
      961 aaacgacggc ggagcgacaa cctgacccga cggtgaatgc gggaggatca gcgacgctcg
     1021 gcttcctcca ggagggccag aaggtgccga cagatcgcct ggccggtccg cgtctgctcc
     1081 ggtgagtggt cggggagccg gcgaatggct cgggcgagcc ggttggccgc aatcagatga
     1141 ggatcccgcg aacccaggct ccgggcggct gcgctcgcgc ggcctcgccc cggttcagac
     1201 cggcctgcgt ccacctggcg acgcttcgcc ggcggcgcct cgtccgggaa cagcggcagg
     1261 gattcaaagc ggtcattcat gacgtggaaa tgtaccgaat actgtataaa catacaatac
     1321 tcggcacatc gtttttcgac aggttattag tcgaatgagt agtcaccgtc cgcataaccg
     1381 acccggatgg tggaccccgg gaggaagtcg ccccccagaa tccggctggc cagggggttc
     1441 tcgatgcgtc tttgaatcac gcgcttgagt ggccgggcac cgtaaaccgg gtcgtagccc
     1501 tcctccacca gcgcttccac cgcggccgga gcgatgtcga tcgtgaggtc ctgttcttcc
     1561 aaccgccggc gcagaatgtc gaactggagc ttggcaatct cggcaacgtg ctcgcgcccg
     1621 agcgggtgaa acacgacgat gtcgtccacc cgattcagga actcggggcg gaagtgctgc
     1681 gagaccacgc ccaggaccgt atccttgatt cggtcggcat tgcccgcctc cgtgagcgac
     1741 tgaatggctt cggagccgag gttcgacgtc atgaccagaa ccgtgttccg aaagtcgacc
     1801 gttctcccgt gcccgtccgt gagacgccca tcgtctaaca cctgcagcag aatgttgaac
     1861 acgtcggggt gcgccttttc gatctcatcg agcaggatca gcgagtaggg ccggcggcgt
     1921 atccgctcgg tgagaaaccc gccctcctcg tagcctacgt atcccggagg ggcgccgatg
     1981 agccgcgcaa cggagtgttt ctccatgaac tcggacatgt ccacgcgcac gagcgcttcg
     2041 tcgctgtcga agagcacctg tgccaacgct ttgcacagct cggtcttgcc cacgccggtg
     2101 gggccgagga acatgaacga gccgttgggg cggtcgggat ccgacagccc ggcacgggag
     2161 cggcggacgg catcggcaac gatgcgcacc gcctcgtcct ggcccacgac gcgttcgtgc
     2221 agggcctctt ccagacgcag gagcttgtcg cgctcacctt ccaacagctt ggtgatcggg
     2281 atgcccgtcc acttcgacac gacctctccg atctcttctt cggtaacgct gttgcgcagc
     2341 agctggcgag gctcggcctc gccgttgctg gcagccgcca gctgcttctc gagctccggg
     2401 atcgtgccgt actgcagctc cgacatcctg gcaagatcgc cggcgcggcg cgccacctcg
     2461 aactcgcctc gtgccgcgtc cagatcctcc ttgagatcct gagcgcccct caaggacgcc
     2521 ttttccgcgt tccagatctc ttctagatcc gcgtactctc tctcgagctc ggcaatggag
     2581 ctctgcaggt cctccaggcg ctttcggctc gcatcgtcgc tttcctgctt gagcgcctcg
     2641 gcctccatct tcagctggat gagccggcgc tccagccggt ccatggcttc cggcttggaa
     2701 tccatttcca tgcggatgcg gctcgccgcc tcgtcgatca gatcgatggc cttgtccggc
     2761 agctgacggt cggtgatgta ccgatgagac aacctggccg ccgcaacgac ggccgggtca
     2821 gtgatgtcga ccgcgtggtg aagctcgtaa cgttccttca agcccctgag aatggcgatg
     2881 gtgtcctcca cgctgggctc gtcgacgagc actttctgaa agcgtctctc taacgcggca
     2941 tccttttcga tgtactggcg atactcgtcc agggtcgtcg cgcccacgca gtggagctcg
     3001 ccgcgagcca gggccggctt gagcatgttg cccgcatcca tcgcgccctc ggccttacct
     3061 gccccgacca tggtgtggag ctcgtcaatg aagaggatga cgttgccctc ctgcttggag
     3121 agctcattga gaaccgcctt gagccgctcc tcgaactcac cccgaaactt cgctccagcg
     3181 atgagagaac ccatgtccag ggcgaggatg tgcttgttct tgagcccttc cggcacctca
     3241 ccattgagga tccgctgggc cagtccctcg acgatggccg ttttgcccac gccgggctcg
     3301 ccgatgagca ccgggttgtt cttcgttcga cgctgcagca cctgcaccgt gcggcgaatc
     3361 tcgtcgtcac gcccgatcac cgggtcgagc ttaccctcct cggcctgggc cgtaaggtcc
     3421 accgtgtact tctccagcgc ctcgcgatgc tcctcggcat tgggatcgga gaccgtctcc
     3481 ccgctgcggg cttgagacac gatcgcctcg agcgccgcct tgtccacgcc cgattcgcgc
     3541 agaaccgatt cgaccgtcgg gtcctcgaac agcgcaagga gcacctgctc cgtggagatg
     3601 aaatcgtcgc ccgctttctg ggccaggcga tcggccaggt tcaggacctt cacgaacgcc
     3661 ggggcaaaag acatttcgcc ggtggggctc ttgagcgtcg gcagctggcc aagccgttcg
     3721 ttcaaacgcg tctgcagctc gttcgcgcga gcgccggctc gcaccagctg cggcaggacg
     3781 gagcccgctt tctgctcgac gagaacggca agcaggtgca gcggctcgat cgctgcgtgg
     3841 tccttaccta gagccatgga ctgcgcgtcc gctagggcat tttgcagtct gcttgtcatt
     3901 ttgtccgggc gcatcccaac gctacctcaa cgaaaacctg ctgctacaat gcgtccgatt
     3961 ttcgagattt caatggcccc atggagcaga gaaagcatcc gagggtccag cttcccctcc
     4021 tggtggagct gcaccacccg tcgctgcccc agggcaagct ccgctgcgtt gcccgggacg
     4081 tctccaaagg gggcgtttac gtccatttcg accagcctcc caccctcata cccggcgcca
     4141 agctgcgact gaccctgctg aaccccagcc aggtggagag ccaacccacg cctaccgtgg
     4201 agatgatggt agcgcggact gagccgacgg gtctgggcct tgccttcgtc aatgccacca
     4261 gtcagcacct gtgggctagc gtagagcgtc tgcgtgacga gctggcgatc ggccgggact
     4321 acttccaggt gttccagggc gcggtcgtac tcaacgagca ggatgaggtg ttgctcgtac
     4381 agcagcacgg aaagtggggg tttcccggcg agtacctggt cgtcggcgag gacgcggaaa
     4441 cggcgctgaa ggcgatgttg acccgccgtt tcgctctggc tgacctgacg atcgacgggg
     4501 tcctcaaggt tggcagcggg gctacgatcg acctgcccga ggcagccgtc ttcagggtct
     4561 atcaccgggt gaccacctcg acggcggaga tctcagccag cgcagaggaa gcgtatcgca
     4621 aggtccgctg ggtggaaaac cgacgcagcg ttgcccagat ggcattcgtc agcaaagagc
     4681 tgcgcgagct cgccaacgcc gcgttgctct gggcagccgg gcgccggcgc gccggctgaa
     4741 caggccaagg gggcgaccgg cgcgtcagtc cgcttgcgat ccctgcaccc agtagcgcgg
     4801 cgtatcccgc cgccaccgcc gcaccgagcc aggcgtccgg ctgtcccata ccaacgcccc
     4861 gtgctccgcg gttgagtgga cgcgcgcgcc gacctccgca aagcgtccga ccacatcggg
     4921 atgaggatgg ccgtagcggt tacgtcggcc ggcgctgatg aagaccacgt ccggctccag
     4981 cacgcgcacg aaagccctgg acgacgagga agcgcttccg tggtgggcgg ccagaaggac
     5041 gtccaccgga tccagtgctc gcagcagttg cccttctacg gcggcaccga tgtctccagg
     5101 cagcagcgcg cgttcccggc cgttgtcgat gagcagcacg caggatgcgt cattgctccc
     5161 caggccgcga gcgcgtgcca gcggcggcgg gtgcaggaat cggaaatcaa cgccgtccca
     5221 acgccaggac tggccagccg agcagcgtga ggattctgag tcgggccggg attcgtatac
     5281 ctcacctacg ggcacggcgc ccaccacggc gcccttgccg ccgctgtgat cgatatccgc
     5341 atgactgatc accagggcgt ccagccgcgg ggagccggtg gcaacgaggg tgggtaggac
     5401 gacctgggcc cccaggtcga agccgctggg atagcgcggg cccgcatcga acagcagccg
     5461 atgcctcttc gtgtccacca gcactgcgct gccctgcccc acgtccatcg cgaggactct
     5521 gaactcaccc ctgggcaagc cctgatcagg gggtagcgcc cagaccgccc aggcgaggca
     5581 caacaaaagg tgcctgcccg aaccgccaca cgccagcatg acgagcccgg tagccagggc
     5641 cacgaacacc ggcaacagtc gtggagcggc cacgagcggg ggcagcgacg ccgacgcttc
     5701 gagaacgccc atcagctgcg cgacgaccac ggcagccatc ccgagcgcgg cctcgccgag
     5761 gaacgggacc agctccatca gcagcgcgcc gatcagcgcc gccggaatga ccagaaagct
     5821 gatgacgggc accgcgacga ggttggccac cggtcccgat gagggaatct gtccctggag
     5881 catgaccagc agcgggctga gccccagcag caacgcgtat tgagcgcgaa cgagagctgc
     5941 agccacccca gcgagcctcg gttcccggac gctgcggc
//
LOCUS       DBNHQR010000027         3147 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_319547, whole genome shotgun sequence.
ACCESSION   DBNHQR010000027 DBNHQR010000000
VERSION     DBNHQR010000027.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_319547"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="AC16VR_00615"
     CDS             complement(<1..755)
                     /locus_tag="AC16VR_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6009679.1"
                     /translation="MTIDHVVRGSGPEPVLVMHDWNGDRRNYDPALPYLDGTTFTYAF
                     VDLRGYGGSKHIAGEHSVAEIAADCLGVADALGWGEFHVVGHSMTGMATQRLALEAPE
                     RVRSAVAVCPVSAAGMAMDDQTRAFFESTTTDDEAFRGLIGMISGSLSGGWVDAKLRW
                     NREAAAPDVRLDYLAMFADTDFAESVEGLETPFLVMVGEHDAEGLDAAAMQKTYLAWH
                     PNAELVVIPNCGHYPMQECPPFFVSTLESFLRRR"
     gene            complement(760..1416)
                     /locus_tag="AC16VR_00620"
     CDS             complement(760..1416)
                     /locus_tag="AC16VR_00620"
                     /inference="COORDINATES: protein motif:HMM:NF014664.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR6009680.1"
                     /translation="MPRLKQISRADAPEHIQRMYGFLFGDRDPVAEPGTDTGTPGNWW
                     TVFALVPDCFDHAVAGFQLYRSPKRELEPTLRELGQTRAGYARGSRFVFSQHCKSLRY
                     LGFSEEQIEAIPHWQAADCFDEVERLVLAFTDCLVLDGGRVPEGLFAALKTHLSDEEI
                     LELTYITCMYDMHATMCRALRLEYDDVEERVSEIAAPEDGGDILTAVDESATGGSAED
                     "
     gene            complement(1481..1747)
                     /locus_tag="AC16VR_00625"
     CDS             complement(1481..1747)
                     /locus_tag="AC16VR_00625"
                     /inference="COORDINATES: protein motif:HMM:NF039725.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6316 family protein"
                     /protein_id="HIR6009681.1"
                     /translation="MNVGRRNEKPKTWFRSERVFMNDGSWFFRTREGVEIGPYETQSE
                     ATMEAGMLRELLRDRGHDGKGIAVIREFVRDSYAMGRPLTPKFD"
     gene            complement(1885..2742)
                     /locus_tag="AC16VR_00630"
     CDS             complement(1885..2742)
                     /locus_tag="AC16VR_00630"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6009682.1"
                     /translation="MNIGAVMAFNHVMSPQFMADAAQMVENAGFHSVWLPEHVLFFPD
                     YESRYPYTEDGRIPGDPEGVLDPFTALTWIAAATKRIRLGTGICLVPQRQPVYTAKMV
                     ADLDYLSGGRVDFGIGIGWLKEEFDNLQMDFRARARRCLEYVEVMQALWSPGLSSYEG
                     ETVTLKPCHFNPKPVQQPHPPIFFGGESDPALARVVSHGQGWYGFNLSPEGLAGRKAR
                     LAEIAAEAGRDMNDVRIFIGPSGKPVTPETVSEYEDLGVEQLIVPVIAGNLERLAQRI
                     DKARAMTGL"
     gene            2857..>3147
                     /locus_tag="AC16VR_00635"
     CDS             2857..>3147
                     /locus_tag="AC16VR_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009683.1"
                     /translation="MSEIALPPRTAYPWYLTSSSLWMAGMSLQGFLFTWLLVGILERP
                     ADEAGLARSLAEFPPLAVLLLGGLLGDRFDNRAYLGVMHLLMAVPPLLIAAVY"
BASE COUNT          558 a         1054 c          973 g          562 t
ORIGIN      
        1 cgtctgcgca ggaaagactc cagagtgctg acaaagaacg gcggacactc ctgcatgggg
       61 tagtgaccgc agttcggaat caccaccagc tccgcattgg ggtgccaggc aaggtaggtc
      121 ttctgcatgg cggctgcgtc gagaccttcc gcgtcgtgct ctccgaccat caccagaaac
      181 ggcgtttcga gcccctccac cgactccgca aagtcggtat cggcaaacat ggcgagatag
      241 tcgagccgca cgtccggggc cgccgcctcg cggttccagc gcagcttcgc gtccacccaa
      301 ccgcccgaaa gcgagccgct gatcatgccg atcagcccgc gaaaggcttc atcgtcggtg
      361 gtggtgctct cgaagaacgc ccgcgtctga tcatccatgg ccatgccggc tgcggacaca
      421 gggcagacgg ccaccgcgct cctgacgcgc tccggggcct ccagggcaag ccgctgggtc
      481 gccatgcccg tcatggagtg accgaccacg tgaaactcac cccagcccag ggcgtccgcc
      541 acacccaggc aatcggcggc gatctccgcc accgagtgct ctccggcgat gtgtttcgag
      601 ccgccataac cgcgaaggtc gacgaaagcg taggtgaacg tcgtcccgtc gaggtagggc
      661 agcgccggat cgtagtttcg gcgatccccg ttccagtcgt gcatgaccag cacgggctca
      721 ggtcccgaac cgcgaacgac gtggtcgatg gtcatgcgct caatcctccg ccgaaccgcc
      781 cgtggcgctt tcatccacgg ccgtcaggat gtcgccgcca tcttccggtg cggctatctc
      841 ggacacccgc tcttccacgt cgtcgtactc gagccgaagc gcgcggcaca tcgtggcgtg
      901 catgtcgtac atgcaggtga tgtaggtgag ctccaagatc tcctcgtccg aaaggtgagt
      961 tttgagcgcg gcgaagaggc cctccggcac ccgaccgccg tccagcacca ggcagtcggt
     1021 gaaagccagc accagacgct cgacctcatc aaagcagtcg gccgcctgcc agtggggaat
     1081 ggcctcgatc tgctcttcgc tgaagcccag ataacgcaga gatttgcagt gctgcgagaa
     1141 cacgaagcgg cttccccggg cataaccggc ccgggtctgg ccgagctcgc gaagggtagg
     1201 ctccagctcc cgcttggggc tgcggtaaag ctgaaagccg gcgacggcgt ggtcgaagca
     1261 atcgggcacc agggcaaaca ccgtccacca gtttcccgga gtgcccgtat cagtgcccgg
     1321 ctcggccacc ggatcgcggt cgccaaaaag aaaaccgtac atccgctgta tgtgttccgg
     1381 cgcatcggct cggctgatct gcttgagacg cggcatgtct aactcctccc aggggtcggg
     1441 gggacattat gcgccgattt gtgggcggcg ggtcaggggg ttagtcgaac ttgggcgtga
     1501 gggggcgacc catggcgtag gagtcgcgga cgaattcgcg gatcacggcg atgcccttgc
     1561 cgtcgtgtcc ccggtcgcgg agcagctctc ggagcatgcc ggcttccatg gtggcttcgg
     1621 actgggtctc gtaggggccg atctccacgc cttcgcgggt gcggaagaac cagctgccgt
     1681 cgttcatgaa gacgcgctcg gaacggaacc aggttttggg cttttcgttt cgtcgcccta
     1741 cattcatcgc ggcaaaaaga cggctaatca ggtcgaacca gtttgcggcg tgaccccctg
     1801 ccagtctgta ggaaagttgt tacggatagt tcccggaccc gccatattcc gcatgatcgt
     1861 gtcatgcctg cgtttgccgg cggctcagag ccccgtcatg gcgcgggcct tgtcgatacg
     1921 ctgcgccagg cgctccaggt tgccggctat caccggcacg atgagctgct cgacgcccag
     1981 gtcctcgtac tccgatacgg tctcgggcgt aacgggcttc ccgctggggc cgatgaatat
     2041 gcggacgtca ttcatatccc gacctgcctc ggcggcaatc tccgccagcc gcgctttgcg
     2101 cccggccagc ccctctggac tcagattgaa tccgtaccac ccctgcccat gcgagaccac
     2161 gcgcgccagc gccggatcgc tctcgccgcc gaaaaagatc ggcggatgag gctgctggac
     2221 cggcttgggg ttgaagtgac acggcttgag agtcaccgtc tcgccttcgt agctggacag
     2281 acccgggctc cagagagcct gcatgacttc cacgtattcg aggcagcgcc gagcccgggc
     2341 cctgaagtcc atctgcaggt tgtcgaactc ctccttgagc cacccgatac cgatgccgaa
     2401 gtccacccgc ccgccggaga ggtagtcgag atccgccacc atcttggcgg tgtagacggg
     2461 ctgacgctgg ggcaccaggc agatcccggt gcccagacgt atgcgcttgg tcgccgcggc
     2521 gatccacgtc agggccgtga aaggatcgag gacgccttcc ggatcacccg ggatacggcc
     2581 gtcttcggtg tagggataac gcgattcgta gtccggaaag aacagcacgt gctccggcag
     2641 ccagaccgag tggaaaccgg cgttctccac catctgcgcc gcgtccgcca tgaattgcgg
     2701 actcatcacg tggttgaacg ccatcaccgc cccgatattc atctcgtcca cctcccagtc
     2761 agtgcagaaa cggcccgcct caacccggct gaagccgccg gaaaccgcta aggttatgat
     2821 gcccgcccgg caaaggacac caccaaggca cccagcttgt ccgagatcgc gctgccccca
     2881 cgcaccgctt acccctggta cctgaccagc tccagcctgt ggatggcggg catgagcctg
     2941 cagggcttcc ttttcacctg gcttttagtg ggcatcctgg agcggccggc ggatgaggcg
     3001 ggcctcgccc gatcgctggc cgagttccca ccgctggccg tcctgctgct cggcggcctg
     3061 ctgggcgacc ggttcgacaa tcgggcctat ctgggagtca tgcacctgct catggccgtt
     3121 cccccgcttc tgatcgccgc cgtttac
//
LOCUS       DBNHQR010000028         4072 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_826548, whole genome shotgun sequence.
ACCESSION   DBNHQR010000028 DBNHQR010000000
VERSION     DBNHQR010000028.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_826548"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="AC16VR_00640"
     CDS             <1..283
                     /locus_tag="AC16VR_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HIR6009684.1"
                     /translation="VQFSVANIVEPGTFEMSDMETIRSPGLCFFLQLPGPENPLEAFE
                     TMLSVAHGVAEELNGELKDEQRSFMTPQTEEHYRQRILEFSRRRMSKRA"
     gene            280..2322
                     /gene="ligA"
                     /locus_tag="AC16VR_00645"
     CDS             280..2322
                     /gene="ligA"
                     /locus_tag="AC16VR_00645"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007152327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HIR6009685.1"
                     /translation="MTITRAGAGSRISELRRLVRHHNHRYYVLDSPEIADAEYDALYD
                     ELVALEGDHPDLVTDDSPTQRVGGAPLEQFNSVTHEVAMLSLDKCTTHEELADWIERC
                     RGRLEEGEALDFVCEPKVDGVAVALTYENGRLVLGATRGDGQTGEDITANVRTVGAIP
                     LVMRDDAGHDFPPRIEVRGEIYMPVADFEAFNEQARQRDEKTLVNPRNGAAGSLRQLD
                     PKVSASRPLTMYCYSIGWVEGDWQPRTHMEALERLADWGFRVNPDARAVSGLAGCLRY
                     VEEMLERRDSLGYDIDGVVIKVNDLDQQRRLGAVTRKPRWSIAFKYPAEEATTTLIGV
                     EFQVGRTGAITPVARLAPVFVGGVTVSNATLHNMDEVRRLDIRVGDTVMVRRAGDVIP
                     QVVSVIDSRRPPGAEAVALPQGCPSCGSPITRPDDEAVARCSAGPEICPAQRKEGLRH
                     FASRLALDIEGLGDKLIEQLVDKDQVRIAADLFSLRKDELIRLERMGAKSAENLLAAI
                     ERSRETTLARFIYALGIREVGEATAQSLARHFGDLEPLRRAQVAELEQVPDVGPVVAG
                     NIHSFFANDDGARAVDALLEAGIRWPAVAVETGAQPLLGQTWVLTGTLESMTRDAAKS
                     ELVALGAKVSGSVSGKTSQVVAGPGAGSKLEKARDLGVPVMDEAEFLEVLESHRHG"
     gene            2315..2932
                     /locus_tag="AC16VR_00650"
     CDS             2315..2932
                     /locus_tag="AC16VR_00650"
                     /inference="COORDINATES: protein motif:HMM:NF039960.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HIR6009686.1"
                     /translation="MGRARVARAAVLGLSVVVMAGCASAPPSKPDQICSIFREKSGWY
                     KDARRAEKRWDMPISVGMAFVYKESSYRSDARPERERLLWVVPWRRPSSAFGYAQITD
                     AAWREYLDDTRGFLRERDDFGTALDFVGWYNDRSHRRLGIAKNDAYNLYLAYYVGHGG
                     YKRGHHRKKPEVRRYARRVSEKSKRYAAQLSRCEKEFRKPWWRPI"
     gene            2978..>4072
                     /locus_tag="AC16VR_00655"
     CDS             2978..>4072
                     /locus_tag="AC16VR_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR6009687.1"
                     /translation="MSEALLNDELKGVIQTGYRAWLDARGFRPRRGQREMIALIARTL
                     TGDAPRIVAVEAGTGTGKTAAYCLAAIPTGAALDKTVVISTATVALQEQVVLRDLPDL
                     ASRSGLTFSFTLAKGRGRYVCLKRLDDRLRFAEPSEIPLFEDTGEEGAALYQAMLNAF
                     ANKTWNGDRDEWTEEVDPQLWRAVTTDHRGCSNNRCGFFRQCPFFKARGALDAADVIV
                     ANHDLVLADLSLGGGVVLPPPEDCIYVLDEAHHLPEKTQQHFSASTRVLATGQWVDNV
                     ARVIGSLTQRFGRPDELVSLASRASEHGAAFAEAMMRMGDDLQGLSFEPRGEGLATHR
                     FRLGQVPAELAEQAQLALEPLTLLAGVISAV"
BASE COUNT          698 a         1294 c         1424 g          656 t
ORIGIN      
        1 agttcagttc agcgtggcga acatcgtcga gccggggacg ttcgagatgt cggacatgga
       61 aaccatccgc tcgccgggac tctgcttctt tctgcagctg ccgggacctg aaaatccgct
      121 cgaggcgttt gagaccatgc tgtctgttgc ccacggcgtc gccgaagagc tcaacggcga
      181 gctgaaagac gagcagcgca gcttcatgac gccccagacc gaggagcact accgccagcg
      241 gatcctggag ttctcgcggc ggcgcatgtc caagcgggca tgaccatcac cagggccgga
      301 gccgggagcc gcatcagcga gctccgtcgg ctggtacgcc accataacca tcgttactac
      361 gtgctcgatt ctccagagat cgccgacgcg gagtacgacg cgttgtacga cgagctcgtt
      421 gctctggagg gcgatcatcc cgatctcgtg accgacgatt cgcccaccca gcgggtcggg
      481 ggggcgccgc tcgagcagtt caactcggtc acccacgagg tggccatgct ctccctggac
      541 aagtgcacga cccacgagga gctcgcggac tggatcgagc gttgtcgcgg acgtctggaa
      601 gagggagaag cgctcgactt cgtctgcgag cccaaggtgg atggcgttgc ggtcgccctg
      661 acctacgaga acggtcggct ggttctcgga gccacccggg gggacggaca gacgggagag
      721 gacatcaccg ccaacgttcg caccgtcggc gcgattcccc tggtaatgcg ggacgatgcg
      781 gggcacgact ttccgccgcg catcgaggtg cgcggcgaga tctacatgcc cgtcgccgat
      841 ttcgaggcgt tcaacgagca agcgcgtcag cgcgacgaga agacgctggt caacccgcgc
      901 aacggagccg cgggcagcct tcgccagctg gatccgaagg tgagcgcaag ccgcccgttg
      961 accatgtact gctacagcat cggctgggtg gaaggagact ggcagccccg gacgcacatg
     1021 gaagccctgg agcgtctcgc cgactggggt tttcgtgtca atcccgacgc ccgtgcggtg
     1081 agcgggctcg ccggatgcct gcggtacgtg gaggaaatgc tggagcgccg cgactcgctg
     1141 ggctacgaca tcgatggtgt cgtgatcaag gtgaacgacc tcgatcagca gcggcgcctg
     1201 ggggccgtca cccgcaagcc gcgatggtcc atcgccttca agtacccggc ggaagaagcg
     1261 accacgaccc tgattggcgt ggagtttcag gtgggtcgta ccggagccat taccccggtg
     1321 gctcgtctgg cgcccgtctt cgtgggcggc gtgacggtga gcaacgcgac gctccacaac
     1381 atggacgagg tccggcgcct ggacatccgc gtgggcgaca ccgtcatggt gaggcgggcg
     1441 ggcgacgtca ttccccaggt cgtcagcgtc atcgattcca gacgcccgcc cggcgccgaa
     1501 gcggtggcgc tgcctcaggg gtgcccgtcg tgtggcagtc ccatcacgcg cccggacgac
     1561 gaggcagttg cgcgctgctc ggccggcccc gagatctgtc ccgcccagcg caaagagggc
     1621 ctgcgccact ttgccagccg tctggcactg gacatcgagg gcctcggcga caagctcatc
     1681 gagcagctgg tggacaagga tcaggtgagg atcgctgcgg atctcttcag cctgcgcaag
     1741 gacgagctga tccgtctgga gcgcatgggc gccaagtcgg ccgagaacct gctggccgcc
     1801 atcgagcgca gccgcgagac cacgcttgcg cgtttcatct acgctctggg cattcgcgag
     1861 gtgggggaag cgactgccca gagcctggcc cgtcatttcg gcgatctgga accccttcgg
     1921 cgggcgcagg tagccgagct cgaacaagtg ccggacgtgg gcccggtggt cgccggcaac
     1981 attcatagct tctttgccaa cgacgacggc gcccgggccg tagacgcgct gctggaggcg
     2041 ggaatccggt ggccggcggt agccgtcgag accggcgcgc agccgctcct cgggcagacc
     2101 tgggtgctga ccggcacgct ggagagcatg accagggatg cggccaagtc ggagctcgtt
     2161 gccctgggcg ccaaggtgag cggctcggtg tcggggaaga cgtcccaggt ggtggccggc
     2221 ccgggcgccg gcagcaagct ggaaaaggcg cgcgacctgg gggtgccggt gatggacgaa
     2281 gccgagtttc tcgaggtcct ggagagccac cgccatgggt agggctcgcg tcgctcgggc
     2341 cgccgttctc ggcttgagcg tggtcgtgat ggccgggtgc gccagcgcgc cgccgtcgaa
     2401 gccggatcag atctgctcga tcttccggga aaaatccggt tggtacaaag acgccaggcg
     2461 tgccgagaag cgctgggaca tgcccatctc cgtgggcatg gcgttcgtct acaaggagtc
     2521 gtcctaccgg tccgacgcga ggccggagcg tgagcgcctg ttgtgggtag tgccctggcg
     2581 gcggccgtcc tcggcgtttg gttacgcaca gatcaccgat gccgcctggc gggaatacct
     2641 cgacgacacc agagggttcc tgcgcgagcg cgacgacttc ggcacggccc tggatttcgt
     2701 cggctggtac aacgaccgca gtcaccggcg tctcggcatc gccaaaaacg atgcctacaa
     2761 tctctatctc gcctactacg tcggccatgg cggctacaag cggggacatc accggaagaa
     2821 gccggaggtc cgccgttatg ctcgacgggt ctcggagaag agcaagcgat acgcggcgca
     2881 gctgtcgcgc tgcgagaaag agttccgcaa gccctggtgg cggccgattt gagcgttgcc
     2941 ggtggccagt gatcgaccgg caacgtaagc tcgctcgatg agcgaggccc tcctcaacga
     3001 cgagctgaaa ggcgttattc agaccggata ccgtgcctgg ctcgacgcga ggggctttcg
     3061 gccccgacgc ggccagcggg agatgatcgc gctgatcgcc cggacgctga ccggtgacgc
     3121 gccgcgcatc gtggccgtgg aggcgggcac tgggacgggc aagacggcgg cctattgcct
     3181 ggcggccatc cccacgggcg ctgctctgga caagacggtg gttatcagca ccgcgacggt
     3241 ggccctgcag gagcaggtgg tcctgcgcga cctccccgat ctggcgagcc gttctggcct
     3301 gacgttcagt ttcaccctgg ccaaagggcg ggggcgctac gtctgcctga agcggctcga
     3361 cgatcggctg cgatttgcgg agccctctga gatccccctg ttcgaggaca cgggcgagga
     3421 gggcgcggcc ctttaccagg ccatgctcaa cgcgtttgcg aacaaaacct ggaacggcga
     3481 tcgcgacgag tggaccgagg aggtcgaccc gcagctgtgg cgggccgtga ccacggacca
     3541 tcggggatgc agcaacaatc ggtgtggctt ctttcggcag tgcccgttct tcaaggccag
     3601 gggagcgctc gacgccgccg acgtcatcgt cgccaaccac gacctggtgc tggccgacct
     3661 cagcctcggt ggcggcgtcg ttctgccgcc gccggaggac tgcatctatg ttctcgacga
     3721 ggcgcaccat ctgccggaga aaacccagca gcatttctcg gcctccacgc gggtcctcgc
     3781 caccgggcag tgggtggaca acgttgcccg ggtcatcggc agcttgacgc agcgtttcgg
     3841 gcgaccggac gagctcgtca gtctcgccag cagggcctcg gagcacggcg ccgcgttcgc
     3901 tgaagccatg atgcgcatgg gggacgatct ccaggggctc agcttcgaac cgcgcggtga
     3961 aggcctggca acgcaccgct tcaggctggg acaggtacca gcggagctgg cagagcaggc
     4021 gcagctggcg ctggagccgc tcacgctcct tgccggcgtg atctccgccg tc
//
LOCUS       DBNHQR010000029         7726 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_953317, whole genome shotgun sequence.
ACCESSION   DBNHQR010000029 DBNHQR010000000
VERSION     DBNHQR010000029.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7726)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7726)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7726
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_953317"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(922..1317)
                     /locus_tag="AC16VR_00660"
     CDS             complement(922..1317)
                     /locus_tag="AC16VR_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR6009688.1"
                     /translation="MNSTPCVRDYMSRSLVTLTPDMRIQDAIEYLVEHEYSGAVVVDV
                     SGKLAGMLSNKDCLKLAFEAGYHQDWPGTVGEYMSSPVETVDADLDVVAAAELFGKGH
                     YRRYPVLEAGVLVGQISRYDILRALKEMW"
     gene            complement(1497..2756)
                     /gene="nqrF"
                     /locus_tag="AC16VR_00665"
     CDS             complement(1497..2756)
                     /gene="nqrF"
                     /locus_tag="AC16VR_00665"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HIR6009689.1"
                     /translation="MAEAASMLEIGFGIGLFTTVMTLLALLVELARTRLLPSIPVRID
                     MGEAGAIQGAAGRTLLEILNDAGVLLPSACAGRGSCGLCRIKVTRGGGVANPVEDSRL
                     GRRELTAGMRLACQVRVRGDLEVQVPDEVFGVRHYTCHVASIRSLTPLVKEVVLTVSP
                     GEAQEIRPGAFVQVTAPPHALAFRAMDMAPWAREKWDALRLWDLESGSDEPTTRAYSL
                     ANTPDEIGTLILFVRLALPPAGAPEEVLPGIVSSWLFARQVGDPIDVSGPYGHFFPSD
                     SDRELIYIGGGVGMAPIRSHIFYLLKHRGSRRTISFWYGARSRIELFNDDEFDGLART
                     YPNFTWQAVLSEPQLGDDWQGPTGFVHEVLYEHYLGKHPAPESCEYYLCGPPLMVRAV
                     LNMLDGLGVDPDRIVFDDFAAADVKRR"
     gene            complement(2749..3357)
                     /gene="nqrE"
                     /locus_tag="AC16VR_00670"
     CDS             complement(2749..3357)
                     /gene="nqrE"
                     /locus_tag="AC16VR_00670"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HIR6009690.1"
                     /translation="MDLLALFVDAIFVDNLALSFFLGICTFLAVSRTVSTALGLGAAV
                     ILVQSVTVPVNNLVHRHLLAEGAWSWAGLPTVDLTYLGLMLYIGVIAAVVQVLEMVLD
                     RYFPTLHRTMGLFLPLLAVNCAILGGSLFMVERDYGFAESVFYGVGSGVGWAAAVVLL
                     AGIREKLRYADVPEGLHGLGLSFIVVGLMSLGFLALADVRLG"
     gene            complement(3332..4027)
                     /locus_tag="AC16VR_00675"
     CDS             complement(3332..4027)
                     /locus_tag="AC16VR_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014421083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HIR6009691.1"
                     /translation="MDQRDRRTLLDPLIDNNPITLQILGLCSALAVTTTVATALTMSV
                     ALTTVLTFSAGIISALRNHIPRSVRLIVQITVISSLVLMVDEVLQAYAVEMSKRLSVF
                     VSLIVTNCIVLGRTEGFAMQHGVKRSLLDGLGNGLGYSLVLILVGIVRELLGSGTLLG
                     LTVFPLAADGGWFQPIALMLMPPSAFLLIGLFIWALRTWRRAQIEPSELPESALVLSE
                     TEALRDGLTRSVR"
     gene            complement(4031..4834)
                     /gene="nqrC"
                     /locus_tag="AC16VR_00680"
     CDS             complement(4031..4834)
                     /gene="nqrC"
                     /locus_tag="AC16VR_00680"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit C"
                     /protein_id="HIR6009692.1"
                     /translation="MHEIGTFTGDGGRTSLARALGVAFAVALVGGSLVSVTAVLLEPR
                     QEANRLAERRAVIDAIVSRLPGVERLLDPASGDRIETRLVDLATGHYVSDAADPAAYD
                     AVRAAQDPSSRVEIPPELDIARIGARAPFAAVHLARRGRSTELVILPVFGSGYVSTLR
                     GFVALEEDMNTITALAFYEHGETPGLGARLQDPVWLAKWRGKRLRDEVGRYRLEVTRG
                     TADAAAAHAVDGISGATRTSQGVSRLLEYWLGDHAFGPFLQRLREEGGH"
     gene            complement(4827..5957)
                     /locus_tag="AC16VR_00685"
     CDS             complement(4827..5957)
                     /locus_tag="AC16VR_00685"
                     /inference="COORDINATES: protein motif:HMM:NF003756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HIR6009693.1"
                     /translation="MSAGTHGRFAHRSPPTLQLRRRLDLSGVMAWVVVALIPSVAVGV
                     WNTGHQASAAIVELGAAAPADWRVATLEGLGLGQWGILSDVIHGALYVVPLLLVATLL
                     GYAWEYLFARVRRRPMGPDTLVTVLIFTLLLPPATPLWQCALGVSFAMVFGKLIFGGT
                     GRNFLNPALTGLAFLYFSFPTHMVGDPMWPLVAGYAGTTAYAIAAEGGMPALTAAGFT
                     WWDAFLGRVPGLLGQTSVLAALIGAAILIWRRIASWRIMLGMLVGAATMGLVFNTAEL
                     GAHSAFTVPWHWHLVLGGVAFGAVFVATDPVSAPMTDPGRWLYGSLIGIAVVVIRLVN
                     PAHPDGVLLAVLLGNLCAPLIDHFTIRLHVRRRAYRRSDRGA"
     gene            complement(5954..7231)
                     /locus_tag="AC16VR_00690"
     CDS             complement(5954..7231)
                     /locus_tag="AC16VR_00690"
                     /inference="COORDINATES: protein motif:HMM:NF048463.2"
                     /note="uses the energy from reduction of ubiquinone-1 to
                     ubiquinol to move Na(+) ions from the cytoplasm to the
                     periplasm; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit A"
                     /protein_id="HIR6009694.1"
                     /translation="MRELSYEIRKGLDLRLPGAPHSSIDEGARVSRVAVLGSDYPSVR
                     PRLVVRQGERVRTGQTLAIDRRRPEVRITSPATGKVAIDVGNRMRLDKIVISVECEDA
                     ERHAVPDADDVATAAPEDLEHALLASGLWTALRTRPFGNIPDPGTRPADIFVTAMDTH
                     PLAPPPPVVIGEREKEFTLGLEALVRLTEGWLYLCQAPGPTLPGLEHERIIVARFAGP
                     HPAGLPGTHIHLLAPVCQERTVWYIGYQDVIGIGALLETGRRPVERVVALAGPVVHRP
                     RLIRTRIGAALDELVVGELVANSHPRAYQIVSGSPLSGCAVDPGEGFLGRLHQQVCAF
                     SADPGGDWVLSNDGPRYPFVPMPDYENVMPLDLLPGPLLRSLLIEDDETAVALGALEL
                     DEEDLALLSYVCPSRQDYGRLLRAALSRVQTRA"
     gene            7349..>7726
                     /locus_tag="AC16VR_00695"
     CDS             7349..>7726
                     /locus_tag="AC16VR_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009695.1"
                     /translation="MMAHYRSFLVFVTMLEVAAFGVARAEPATSGGGFEIPSERVSAD
                     ARARGYVGPRTCANCHVAQYDAYRGSGHPKKLRLAEEARAAGLPLPEGYRWNDISYVV
                     GGHRWKARYVDVQGFIITSTGPGR"
BASE COUNT         1479 a         2541 c         2467 g         1239 t
ORIGIN      
        1 cggactcgaa tgttgtgtgc attgcaatga cgagcgccac cagtgccatg tcaacaagga
       61 gtgcaatcgg tttcagggcc caagtgtttc gccgaaagcc gtagttcatg ttctcctcga
      121 aaatgagtcg gaactcttgt ttgtcgcttg ttgcggacaa aagccaatcg gtggctgatg
      181 cgtagatctc gtccgctgct ttcgggtcac caaattcctc ttcatggttg gggattctga
      241 gattgggtac atgcgccgaa aggaacgcgt gataccgttt tttgttatgg cgctaattcc
      301 ggtgtcccga tggcgcagca gggcaacaga aggcatcccg ccccagtggt gatacaactt
      361 gcgctcttgc ttcttaccgc gtttgcggcc gatctgtgtg agccacgccg cgcccccaaa
      421 gtaggcgacg atgcccgccc atgttgcgac cctcgtttcc agccctgggt acaggaccag
      481 cgcgaccact gcgacggcta gcagagcgaa caacgctggc tgcaatcgtg cgcggcgatt
      541 gtaagagtcg gttgttgtct tgcgggtcat tcagagcgag ccgtgcgccc agaaatatgt
      601 cgtcctcaag cggccgtagc cgctggcccc acgaagcacc tcttcacagc aaactctggg
      661 actccccgcg aattcccaat tcctggacac ctcgttgtgt acgtcgctct gctgcccctg
      721 ataatcggct catgacccat cccgtactag gccaccgggg aatagcttcg ctgttgtttc
      781 agggtgatct ccttctaacc ggggtaggcg ctccattcca cgaaagcgcc ccgcattccc
      841 tctgcacgcc ggaacggcag agcctttgcg agccgcaccg ccagaggcga cggcatactt
      901 catcgagtta ggggggccgg atcaccacat ctctttcagt gcgcggagaa tatcgtagcg
      961 gctgatttgc ccgaccagga cacccgcctc gagcaccggg taccggcggt agtgtccctt
     1021 gccgaaaagt tcggccgccg cgaccacgtc gaggtccgcg tcgacagtct ccaccgggct
     1081 ggacatgtat tcgcccacgg tgcccggcca gtcctggtga taaccggcct cgaaggctag
     1141 cttgagacag tctttgtttg acagcattcc ggcgagcttt ccagagacgt ctaccacgac
     1201 ggcacccgag tactcatgct cgaccaggta ctcgatcgcg tcctgaatcc tcatgtcggg
     1261 ggttagcgtg acgagagacc gggacatgta atcgcgaacg cacggcgtgc tattcatcgg
     1321 ctcgctcctc tctgtacggg cagccgctcg aggtccgaag cggacacctc cctgacagtg
     1381 cgcacgtgtt gccgcaacca gagcgaagcg gtcggccacg cgttatcaga ccgatgccga
     1441 gcagcagcac cgcgagcagg aaaaccgaca gcccgagaat gatggtgatc acggcctcac
     1501 cttctcttca cgtcggccgc tgcgaaatcg tcgaacacga tccggtcggg gtcgacgccg
     1561 agtccgtcca gcatgttgag caccgccctc accatcaacg gcggtccgca cagataatac
     1621 tcgcaggatt cgggtgcagg atgcttcccc aggtagtgct cgtaaagcac ctcgtgcacg
     1681 aaccccgtgg gcccttgcca atcgtccccg agctgcggct ccgacaggac ggcctgccag
     1741 gtgaagttcg gatacgttcg agcaaggcca tcgaactcgt catcgttgaa caactcgatg
     1801 cggctgcgcg ccccgtacca gaagctgatg gtacgccggc taccgcgatg cttcagcaaa
     1861 tagaagatgt gcgagcgaat tggcgccatg cccacgccgc cgccgatgta gatcagctcc
     1921 cggtccgagt cgctcgggaa gaagtgaccg tagggtcccg aaacgtcgat cgggtctccc
     1981 acctgacggg cgaacaacca tgaggatacg atgccgggca gcacttcctc gggtgcgccc
     2041 gcgggcggca gggcaaggcg cacgaagagg atcagcgtgc cgatctcatc cggcgtgttg
     2101 gccagcgagt aggcccgggt ggtgggttca tcggatccgc tctccagatc ccaaagccgc
     2161 aacgcgtccc atttctcccg cgcccaggga gccatgtcca tcgcgcgaaa cgccaaggcg
     2221 tggggcggtg cggtgacctg cacgaaggcg cccggtctga tctcttgcgc ctcgccggga
     2281 gaaacggtca gaaccacctc cttgactagc ggggtcaaac ttcggatgga ggccacgtgg
     2341 cacgtgtaat gccgcacccc gaacacctcg tccggtacct gcacctcgag gtcgccccgg
     2401 acccgaacct ggcacgcgag tcgcatcccg gccgtgagct cgcggcggcc gagccgcgag
     2461 tcctcgaccg ggttggcgac gccgccgcct cgcgtgacct tgatacgaca cagcccgcag
     2521 ctgccacggc cagcgcacgc ggagggcagc aacacacccg catcgttcag gatttcgagt
     2581 aacgtgcggc ccgccgcgcc ctgaatcgct cctgcttcgc ccatatcgat ccgcaccgga
     2641 atgctcggca gcagacgcgt acgggccagc tcgacgagga gcgccagcag cgtcatcacc
     2701 gtggtgaaca gcccgatgcc gaatccaatt tcgagcatgc tcgcagcctc agccaaggcg
     2761 gacgtcggcg agggcgagga agccgagcga catgagccct accacgatga agctgagacc
     2821 gagaccgtgc agcccttccg ggacgtccgc gtagcgcagc ttctcgcgga tacctgcgag
     2881 cagaacgacg gccgccgccc atccaacccc ggagccgacg ccgtagaaga cgctctccgc
     2941 gaacccgtaa tcccgctcca ccatgaacag ggagccgccg agaatggcgc aatttacggc
     3001 cagcagcggg aggaacaacc ccatggtgcg atgcagggtc ggaaagtaac gatccagaac
     3061 catctccaac acctggacga cggcggcgat caccccgatg tacagcatca ggcccaggta
     3121 ggtcagatcc accgtcggca gccccgccca gctccacgca ccctcggcca agaggtggcg
     3181 gtgcaccagg ttgttcacgg gcacggtgac gctctgcacg aggatgaccg cggccccgag
     3241 gccgagtgcg gtggagacgg tgcgcgacac cgccagaaag gtgcagatcc cgagaaagaa
     3301 cgagagcgcc aggttgtcga cgaatatcgc gtcaacgaac agagcgagta agtccatctc
     3361 tcaacgcctc cgtctcggaa agcacgagcg cgctctcggg cagctccgag ggctcgatct
     3421 gggcccgcct ccaagtccgg agcgcccaga tgaacaagcc aatcagcagg aacgcgctcg
     3481 gcggcataag catcaaggcg atgggttgaa accagccccc gtcggctgcc aacgggaaga
     3541 cggtcagccc aagcagcgtc ccggagccca gcagctcccg cacgatgccc accaggatca
     3601 gcaccaggct gtaccccagg ccgttgccca gaccgtcgag caggctgcgc ttgacaccat
     3661 gctgcatggc aaagccctcg gttcgcccca gcacgatgca gttcgtgacg atcaggctca
     3721 cgaataccga gagtcgcttg ctcatctcga ccgcgtaggc ttggaggacc tcgtcgacca
     3781 tcagcaccag ggaggaaatg accgtgatct ggacgatcag ccggacgcta cgcggaatgt
     3841 gatttctgag cgcgctgatg atgccggcgg agaaggtcaa gaccgtggtc agcgccacgc
     3901 tcatcgtcag cgcggtggcg acggtcgtag tcaccgcgag tgcggagcac aagcccagga
     3961 tctgcaacgt gatcggattg ttgtcgatca atggatcgag cagagtgcgc cggtcccgct
     4021 ggtccatcgg tcagtgacct ccttcttcgc ggagccgctg cagaaagggc ccgaaggcgt
     4081 gatctccgag ccagtactcg agcagccggg agacgccctg gctggtgcgg gtcgcaccgc
     4141 tgatcccgtc gaccgcatgc gcggcggcgg cgtccgcggt tccgcgggtc acctcgagtc
     4201 gataccgtcc cacctcgtcg cgcaatcgct ttccgcgcca cttcgcaagc cacaccggat
     4261 cctgcaaccg cgcaccgagc ccgggcgtct cgccgtgctc gtagaaagca agcgcggtga
     4321 tggtgttcat gtcttcctcc aaggctacga agccgcgcag ggtagacacg tagcccgagc
     4381 cgaagacggg caggattacg agctccgtgc tgcgacccct gcgcgcgaga tgaaccgcgg
     4441 cgaacggtgc acgtgcgccg atccgggcaa tgtcgagctc gggggggatc tcgacccggc
     4501 ttgaggggtc ctgcgcagcc ctaaccgcat cataggccgc tggatcggcc gcgtccgaga
     4561 cgtagtgccc ggtggcgaga tccacgagcc gcgtctcgat ccggtccccg ctggcgggat
     4621 cgagcaagcg ttccaccccc ggcaggcgcg agacgatcgc atcgatgacg gcgcggcgct
     4681 ccgcgaggcg gttcgcctcc tgacgtggct cgagcaggac cgcggtcacg gagaccaggc
     4741 ttcccccgac cagggccacc gcgaacgcca cgccgagggc acgggctagg ctggtgcgcc
     4801 caccgtcgcc cgtgaaggtg cctatctcat gcaccgcggt cgctcctgcg gtaagcgcgc
     4861 cttcgcacgt gaagccgaat cgtgaagtgg tcgatcagag gtgcgcagag gttgcccagc
     4921 agcacggcga gcagcacgcc gtcgggatga gcgggattga caagtcggat gacaaccacc
     4981 gcaatcccga tcaggctgcc atagagccag cgcccgggat cggtcatggg cgcgctgacg
     5041 ggatccgttg cgacgaaaac ggcaccgaac gccactccgc cgagcacgag gtgccagtgc
     5101 cacggcacgg tgaacgccga atgggccccg agctcggctg tgttgaaaac gagccccatg
     5161 gttgccgctc ccacgagcat ccctaacatg atgcgccacg acgctatccg ccgccatatc
     5221 agaatcgccg ccccgatcag cgccgcgagg acggacgtct gccccagcaa gccgggcacc
     5281 cgtcccagga aggcgtccca ccacgtgaag ccggccgccg tgagtgcggg catgcctcct
     5341 tcggcggcga tggcgtatgc cgtcgttccc gcgtagccgg ccaccagggg ccacatcgga
     5401 tcgcccacca tgtgggtagg aaagctgaag tacagaaaag ccaaaccggt gagcgccggg
     5461 ttgaggaagt tccggcccgt gccaccgaag atgagcttgc cgaacaccat ggcaaacgag
     5521 acccccagcg cgcactgcca aagcggtgtc gcgggcggta gcaggagcgt gaagatcagg
     5581 acggtgacca gggtgtcggg gcccatcggg cggcggcgca cgcgcgcgaa caggtactcc
     5641 cacgcatagc cgagcagcgt cgcaactaag agcaacggca ccacgtagag cgcgccatgt
     5701 atgacgtccg aaagaatacc ccattgcccc aagccgagcc cctccaacgt cgcgacgcgc
     5761 cagtctgccg gagccgcggc gccgagttcg acgattgcag cgctcgcctg gtggccggtg
     5821 ttccacactc ccacagccac gctggggatc aaggcaacga cgacccaggc catcacgccg
     5881 gacaggtcga gccgccggcg cagctgtagc gtcggcggcg atcggtgcgc gaatcgcccg
     5941 tgtgtgcccg cactcatgct cgtgtctgca ctcgcgagag ggccgctcgc agcaaccggc
     6001 cataatcctg cctagacggg cacacatagc tgagcaaggc caggtcctcc tcatccagct
     6061 cgagcgcgcc aagcgccacc gcggtctcgt cgtcctcgat gaggagcgag cgcagcaggg
     6121 ggccggggag caggtcgaga ggcatgacgt tttcgtagtc gggcatcggg acgaaagggt
     6181 atctggggcc gtcgttggac aggacccaat cgccacccgg atctgcgcta aaggcgcaga
     6241 cctgctggtg gagtcttcct aggaatccct cgcccgggtc gaccgcacat cccgagagcg
     6301 gcgagcccga gacgatttgg taagcgcgcg ggtgagaatt cgctaccagc tcccccacga
     6361 cgagttcgtc caaggcggcg ccgatgcggg tccttatcag ccgcggacgg tggaccacgg
     6421 ggccagccag cgccaccacg cgctcgacgg gacgtcgtcc tgtctcgagc agggcgccga
     6481 tcccgatgac gtcctgatat ccgatgtacc aaaccgtccg ctcttgacag accggtgcga
     6541 gcaggtggat gtgcgtgccc ggtaggccgg ccggatgggg ccccgcgaag cgggccacga
     6601 tgatccgctc gtgctctagt cccggcagcg tggggcccgg cgcctggcac aggtagagcc
     6661 aaccctcggt gagtcgcacc agtgcctcga ggcccaacgt gaactccttc tcgcgttcgc
     6721 cgatgacgac cggcggcggg ggagcgaggg ggtgcgtgtc catcgcggtg acgaagatgt
     6781 cagctggccg ggtgccggga tcgggaatgt tgccgaaggg ccgggtgcga agggccgtcc
     6841 acagacccga ggcgagcagc gcgtgctcga gatcctcggg cgctgccgtg gccacgtcgt
     6901 cggcgtcggg cacggcatgg cgctctgcgt cttcgcattc gacgctgatt acgatcttat
     6961 ccagccgcat ccgattaccg acatcgatcg cgaccttgcc tgtggccgga gaggtgatgc
     7021 gtacctcggg acgacggcgg tcgatggcga gggtctgccc ggtgcgaacc cgctccccct
     7081 ggcgcaccac cagccgcggg cgcacgctgg gatagtccga gccgagcacg gccacacggc
     7141 tcacgcgggc gccttcgtcg atactcgaat gcggcgcacc gggcagcctc aggtccaggc
     7201 cctttcggat ctcgtaactt aactcacgca tgacgtacag cttatctcgg tacggggtgc
     7261 gcccggtacc cggtacgagc ggtcgttgaa tcaactaatg acatgtggga gcctcgtgga
     7321 agaacttaca tagacatcgg aggtcgggat gatggcgcac tatcggtcct tcctggtttt
     7381 cgtgaccatg ctcgaggtcg ctgcgttcgg ggtcgcgcgg gcggagccgg caacatcggg
     7441 ggggggattc gagattccgt ccgaaagggt gtcggccgat gctcgcgcgc gcggatatgt
     7501 ggggccacgc acctgcgcga actgtcatgt cgctcaatat gacgcttacc gaggatccgg
     7561 ccatcccaag aagctacgcc tcgccgagga ggcgcgggcc gccggactcc ccctcccgga
     7621 ggggtaccgg tggaacgaca ttagctatgt ggtcggcggt catcggtgga aggctcgtta
     7681 tgtcgatgtg caaggcttca tcatcacctc caccggaccc ggccgc
//
LOCUS       DBNHQR010000030         3961 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1079880, whole genome shotgun sequence.
ACCESSION   DBNHQR010000030 DBNHQR010000000
VERSION     DBNHQR010000030.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1079880"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1558)
                     /locus_tag="AC16VR_00700"
     CDS             complement(<1..1558)
                     /locus_tag="AC16VR_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003280474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6009696.1"
                     /translation="MIARIIDGAIGERLIVLLGALILAGAGTWAYLTVPVDAIPDLSD
                     VQVIVFTDYPGQAPQVVEDQVTYPLTTAMLAVPKARVVRGYSFFGLSFVYVIFEDGTD
                     MYWARSRVLESLNYVSGRLPAGVNPALGPDATGVGWIYEYALVDRSGRHDLAQLRSLQ
                     DWYLRYPLQTVPGVAEVASVGGFVKQYQVEVDPNALMALGIPLSKIKQAIQRSNNDVG
                     GRLLEISETEYMVRGLGYIRSLEDLGSIPVAADPGGTPIRLGDVARIQIGPELRRGLV
                     DLDGDGEVVGGIVVMRYGENARATIEGVRQRLDELRAGLPEGVEIVTVYDRGDLIDRA
                     VDTLSRSLLIEIVIVSLVVIVFLAHARSAMVAVLILPLGVLIAFLLMRWHGLNANIMS
                     LGGIAITIGAMVDGAIVMVENAHKHMARDAERKGAELSSAERWQAVGAAAREVGPALF
                     FSLLVITVAFLPVFSMQAQEGRLFSPLAFTATYAMAGAAILAITLVPVLMGYLVRGRI
                     RPEESNPINRW"
     gene            complement(1555..2742)
                     /locus_tag="AC16VR_00705"
     CDS             complement(1555..2742)
                     /locus_tag="AC16VR_00705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6009697.1"
                     /translation="MNPSVTSAVPAKDSMGMDYIPVYADEEAAGPAGTVSIDPIVVQN
                     IGVRTARAQRAALSHTVRALGRIDFDERSIARLHPKVEGWVEEMYVDTTGAQVSEDTM
                     LLSVYSPRLVASQQEYVLALRNYESLNDSPIPDISRGAADLVASSRARLELLDVPEHQ
                     IVELEKSRVPQKSLHIHSPAEGVVLKIGARQGQHVTPMTELYVIADLSRVWVYADVFE
                     HDLPWVRVGDAVEMTLGALPGETFGGLLDYIYPYAEAKTRTVKARLVFDNPDRLLRPE
                     MFADVLIRTSTEPNALVIPAEAVVRSGLTNQVFVVRAPGRFEPRVVELGVESEGRVSV
                     LEGLAEGEEVVTSGQFLIDSESKLREAAAKMMARPGDEASSPDGVGDHDHHHGGHDRG
                     ASP"
     gene            complement(2747..>3961)
                     /locus_tag="AC16VR_00710"
     CDS             complement(2747..>3961)
                     /locus_tag="AC16VR_00710"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR6009698.1"
                     /translation="VNFPTDTFHVGQEPMTQLQVGISQTIPFPGKRALREAASSHEAE
                     AARSSVAETRLRLIRDVSFNWWTLHYLDRAQEIVQRNQELLRQFVQIAKTRYEVGGGL
                     QQDVLLAQLELSKLLDQAIQLNGARDVEAARLNRLLDQPANQPIELPRTPVGVFPDLV
                     AQQELYQRGAESRPLLKRLRSEIDAAESRLELARKDYYPDFTVGAAYGARSGENPMPG
                     GGDRTDLLTLRVSVNLPIYQSRKRAQAVSQRRSEMDRHRYALQDEVNAMRAEISQAVA
                     DYRRAREQLVLLDSGIIPQARQTVQSMLAGYQVGEVDFLNLVRSQVTLYNYETLYWKA
                     VTLANQAVARLSAAVGEEVRGGYEISAADDCGARRRGGRGLLLRGAVGGQTERNGTAG
                     AGGEGARTHLLS"
BASE COUNT          739 a         1340 c         1202 g          680 t
ORIGIN      
        1 gccatcggtt gatggggttg gattcctccg gccgaatacg ccctctgacc agatagccca
       61 tgagcacggg taccagcgta atggccagga tcgctgcgcc cgccatcgcg taggtcgccg
      121 tgaaagccag cgggctgaat aaccgccctt cctgggcctg catcgagaat accggcagaa
      181 aggccacggt gatcacgagc agcgagaaga acagggccgg accaacctcg cgagccgctg
      241 cgcccacggc ttgccatcgc tcggccgatg agagctcggc acccttgcgc tcggcgtcgc
      301 gggccatgtg tttgtgcgcg ttctcaacca tgacgatcgc cccatccacc atggcaccga
      361 tggtgatcgc gatccctcca agggacatga tgttggcgtt caggccatgc cagcgcatca
      421 gcaggaaagc aatgagcacg cccagcggga ggatcaagac ggccaccatc gccgaccttg
      481 catgggccag aaagacgatc accaccaagc tgacgatgac gatctcgatg agcagcgacc
      541 ggctgagggt atccaccgcc cgatcgataa gatccccgcg gtcatagacc gtcacgatct
      601 ccacaccttc cgggagaccg gccctgagct cgtccagccg ttggcgcacg ccttcgatgg
      661 tggcgcgcgc attttcccca taacgcatca ccacgatgcc gcccaccact tcgccgtctc
      721 catccagatc caccagccca cgcctgagct cgggccctat ctgaatccgc gccacatccc
      781 caaggcggat gggcgtaccg cccggatccg cggccaccgg tatcgacccg aggtcctcca
      841 gagatcgaat gtagcccagg ccccgcacca tgtactcggt ctcggagatt tccaatagcc
      901 gcccgcccac atcgttgttc gaacgttgaa tggcctgctt gattttcgac agcgggatac
      961 caagggccat gagcgcgttg gggtccacct ccacctggta ctgcttgaca aacccgccaa
     1021 cggaagcaac ctccgcaacg ccaggcacgg tctgcagcgg atagcgcaga taccagtcct
     1081 gaagagacct gagctgcgcc agatcgtggc ggccggaccg atccaccagg gcgtactcgt
     1141 atatccagcc cacgcccgtc gcatcagggc caagcgcggg gttgacgccg gccggcaggc
     1201 ggccgctgac gtagttgagc gactccagaa ctctggatct cgcccagtac atgtcggtgc
     1261 cgtcttcgaa tatcacgtag acgaaagaca agccgaagaa agagtatccg cggaccacgc
     1321 gggccttggg aacggcgagc atcgctgtgg tgagcgggta ggtcacctga tcttccacga
     1381 cctggggcgc ctgtccggga tagtcggtaa acacgatcac ctgcacgtcg gacaggtcgg
     1441 gaatggcatc cacgggcacg gtgaggtagg cccatgtacc cgcaccggcg aggatgagcg
     1501 ctccgagcag aacgattaaa cgctcgccga tcgccccatc gatgatgcgc gcgatcacgg
     1561 cgacgctcca cggtcatgcc cgccgtggtg atggtcgtga tcgccgactc cgtcgggcga
     1621 cgacgcttca tcacccgggc gcgccatcat cttcgcagca gcctcgcgga gcttggattc
     1681 cgaatcgatc agaaactggc cggacgtgac cacttcctcg ccctcggcaa gcccttcgag
     1741 aacactgact cgaccctcgg actccacccc gagctcgacc acccgcggct caaagcggcc
     1801 gggcgcacgc acgacgaaca cctgattggt cagacccgag cggacgaccg cctccgcggg
     1861 aatgaccagc gcgttgggct ccgtcgacgt acggatgagc acgtcggcga acatctccgg
     1921 tctcagaagc cggtccgggt tgtcgaaaac caggcgcgcc ttgaccgttc gggtcttcgc
     1981 ttccgcatag gggtatatgt agtccagcaa ccccccgaat gtctctcccg gcagcgctcc
     2041 cagcgtcatc tcgaccgcat cgcccacccg aacccagggc agatcgtgct cgaacacgtc
     2101 ggcgtaaacc cacacccggg agagatcggc aatcacgtag agctcggtca tgggcgtcac
     2161 gtgctgaccc tggcgggccc cgatcttcag gaccacgcct tcagcaggcg agtgaatgtg
     2221 cagcgacttc tgcgggacgc gggatttttc cagctcgacg atctgatgct ccggcacgtc
     2281 cagcagctcg agccgggcac gggaactcgc gacgagatcg gccgcgccgc gactgatgtc
     2341 gggaatgggg ctgtcgttga gcgactcgta gttcctgagc gcaagcacgt attcctgctg
     2401 gctggccacc aggcgcggcg agtacacgct caacagcatg gtgtcctcac tgacctgagc
     2461 accggtggtg tccacgtaca tttcctcgac ccaaccttcg accttggggt gcagcctggc
     2521 aatactccgc tcgtcgaagt cgatacggcc taacgcgcgc acggtgtggc tgagcgcggc
     2581 gcgctgagcg cgagccgttc tgacgccgat gttctgcacg acgatgggat cgatggaaac
     2641 cgtccccgcc gggccggcag cctcctcgtc cgcgtaaacc gggatgtagt ccatacccat
     2701 ggagtctttc gccgggaccg cagaggtgac ggacgggttc atggggttac gatagaagat
     2761 gggttctcgc gccctcccct ccggctccag cggtgccgtt tcgctcggtt tgcccgccga
     2821 cagctccgcg aagaagtagc ccgcgcccac cccgccggcg agcgccacaa tcatcagcag
     2881 cagaaatttc gtacccacct cgcacctcct cgcccaccgc ggcgctcaac cgcgcgacgg
     2941 cctgattcgc cagagtgacc gctttccagt acagcgtttc gtagttgtag agcgtgacct
     3001 ggctgcgcac gagattcaga aaatccacct cgccgacctg ataaccggcc agcatcgact
     3061 gcaccgtctg gcgcgcctga ggaatgattc ccgagtcgag cagcaccagc tgctcgcggg
     3121 cgcgccggta gtcggcgacc gcttgggaaa tctccgcccg catggcattg acctcgtcct
     3181 gcagcgcgta gcggtgtcga tccatctcgc tgcggcgctg gctgactgcc tgagcgcgct
     3241 tccggctctg gtagatgggc agattgacgc tcaccctgag ggtcaacaga tccgttctgt
     3301 cgccaccccc aggcatcggg ttctcgccgg atcgagcgcc gtacgccgcg cccacggtga
     3361 aatcgggata gtaatctttg cgcgcaagct cgagcctgga ttccgccgcg tcgatctcgc
     3421 tgcgcaggcg cttgagcagc ggacgcgatt ccgccccgcg ctggtacagc tcctgctgcg
     3481 cgacgagatc cggaaagacg ccgacgggcg tcctgggcag ctcgatgggt tggttggccg
     3541 gctggtccag cagacggttc agcctggcag cttccacgtc gcgggcgcca ttgagctgga
     3601 tcgcctggtc gagcagcttt gaaagttcca gctgcgccag caggacatcc tgctgcagcc
     3661 caccgcccac ctcgtagcgg gtcttggcaa tctggacaaa ctggcgcagc agttcctggt
     3721 tccgctgaac gatctcctgg gcgcgatcga gatagtgcag ggtccaccag ttgaaggaca
     3781 cgtccctgat cagccgcagc cgggtctcgg ctacgctgct gcgagcggcc tccgcctcgt
     3841 ggctggaagc cgcttcgcgc agcgcccgtt tgcctggaaa cggtatggtc tggctgatcc
     3901 ccacctgcag ctgggtcatg ggctcctggc ccacgtggaa agtgtccgtg gggaagttca
     3961 c
//
LOCUS       DBNHQR010000031         3662 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_3015, whole genome shotgun sequence.
ACCESSION   DBNHQR010000031 DBNHQR010000000
VERSION     DBNHQR010000031.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_3015"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="AC16VR_00715"
     CDS             complement(<1..533)
                     /locus_tag="AC16VR_00715"
                     /inference="COORDINATES: protein motif:HMM:NF013706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR6009699.1"
                     /translation="MRFLTYRLEGRTAFGAVIDGGIVNLAERLPAFGSLRDLLAAGAL
                     EQARRVAEDADPDHRLQDVGFLPPIPDPQKICCIGVNYGNRNEEYADAAPAARYPSLF
                     LRTRESLVGHLEPIVRPPESEQLDYEGEIVIVIGKAGRRIPESQARAHVAGLTIMNEG
                     TIRDWLRHAKFNVTQGK"
     gene            643..1059
                     /locus_tag="AC16VR_00720"
     CDS             643..1059
                     /locus_tag="AC16VR_00720"
                     /inference="COORDINATES: protein motif:HMM:NF014958.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate isomerase"
                     /protein_id="HIR6009700.1"
                     /translation="MPHLIIEYSANIDEELMLDALMLRLRDSAVATGVFPLPGIRVRA
                     ARRDRFVVADGDPENAFVHLMVRIGHGRTPEVRRQVADRLFAVLCDHLREAATRRGLG
                     LSLEIQEIDPVGSLKRNNLHERLAGRAAGHVSVKVE"
     gene            1056..1691
                     /locus_tag="AC16VR_00725"
     CDS             1056..1691
                     /locus_tag="AC16VR_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009701.1"
                     /translation="MKIGPVLICAFALAMAPFAGAQSVSAWDLVYPSNFNWEIRTHRV
                     RVANDLASRVSLLVAVVPRQTEEESARLRETEQTLEARGADASPRDRSRLYLSRAYQH
                     RRLLELLADTLDALQCVRSAEELPREVHCWTRAAVNLMDEETVDVALTVLRDARMVPR
                     DEDMPVKAQDPKVWYGEYGKGIVRHIVMPYLAGLAAPRTPAATEEEVSHDG"
     gene            1708..2301
                     /locus_tag="AC16VR_00730"
     CDS             1708..2301
                     /locus_tag="AC16VR_00730"
                     /inference="COORDINATES: protein motif:HMM:NF019339.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6009702.1"
                     /translation="MLTLLLSSTLAAASETVLFNESAAFECYQAALHDGGEYDIESCS
                     RALEYQALTTIDRAATHSNRGILYARIGDIRNALRDHNRAVKLAPEVGSIYINRSNTL
                     VRAKRYEGAMRDLEKAIEIADDSLAYAHYNRALLFQKLGDLQAARADAEQAAEIAPDS
                     PAYRRFLAAFGETEGDPLLPDAEPGNPPEDPGGEGQD"
     gene            complement(2292..>3662)
                     /locus_tag="AC16VR_00735"
     CDS             complement(2292..>3662)
                     /locus_tag="AC16VR_00735"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6009703.1"
                     /translation="QEHMGDVEAPLMQMQDEGLISRVLVRVPSWGGTSPNTGMVIVSL
                     EPWRERDATTREIIDRASTAWEEIPAVRIFAFERSGLSRGGGGRPVQFVIGGPNYETL
                     AQWRDLLIARAMEYPGMTRVDSDLKETQPQLIVRVDKNRAAALGISVQNVGRTLAAMM
                     SERRVTTFVQDGEEYDVILQAKEDQRATAADLQNIYVRSESTGRLVPLANLIEVEETA
                     GAGRLNRYNRLRAVTIEAGLAPGYALADALEFLETVVREELPESAQVDYRGESLEFKE
                     ASGSLLFTFAIALLIVYLVLAAQFESFVHPLVILLTVPMAIAGALLGLKLTGGTLNIY
                     SQIGIVMLIGIAAKNGVLIVEFINQLRDAGRSFDDAIVEAAGIRLRPVIMTTISTVMG
                     STPLILATGAGSTSRSMLGVVIFSGVSLATLLTLFIVPAFYHLLARRTGSPEETARRL
                     KGLIQS"
BASE COUNT          632 a         1170 c         1215 g          645 t
ORIGIN      
        1 ttctttccct gagtaacgtt gaacttggcg tgtctcagcc aatcgcggat cgttccctcg
       61 ttcatgatgg tcaagccggc cacgtgagcg cgcgcctgac tctccggtat gcgtcggccc
      121 gctttgccga tgacgatgac gatctctccc tcgtaatcga gctgctcgga ctccggcggt
      181 cgcacgatcg gttccaggtg acctacgagg gattcccgcg tgcgcagaaa aaggctcggg
      241 tagcgggcgg ccggagcggc gtcggcatac tcctcgttgc gattaccgta gttcacgccg
      301 atgcagcaga tcttctgcgg gtcgggaatg ggcggcagaa agccaacgtc ttggagccga
      361 tggtcgggat cggcgtcctc ggccacccgg cgggcctgtt ccagcgcgcc ggctgcgagc
      421 aggtcgcgca gggagccgaa ggccggcaga cgctccgcga gattcacgat gccgccgtcg
      481 atgaccgcgc cgaaagcggt ccttccctcc aaccgatatg tcaaaaatcg cacgcgctct
      541 ccccgtctgg cgcctatgag catagaatgt cagcgattcc ctgatccgga gcgctgtcat
      601 acttcaccct gattcctgca gtgtcaacgc gaggctctcc cggtgcccca tctgatcatc
      661 gagtactcgg cgaacatcga cgaagagctg atgcttgacg cgctcatgct ccggctgcgt
      721 gacagcgccg tcgctacggg cgtgttcccc ctgccaggca tccgcgtgcg cgcagcccga
      781 cgggaccgct tcgtcgttgc cgacggggac ccggaaaatg ccttcgtgca cctcatggtc
      841 aggatcggtc atggccggac gccggaggtt cgccggcagg tcgcggatcg tctgttcgcg
      901 gtcttgtgtg atcacctcag agaggcggct acccggcgcg ggctggggct gtccctggag
      961 atccaggaga ttgacccggt gggaagcctg aagcggaaca acctgcacga acggctggcc
     1021 gggcgcgccg caggccacgt ctccgtcaag gtcgaatgaa gatcgggccg gttctgatct
     1081 gcgccttcgc gctggcgatg gcccccttcg ccggcgcgca aagcgtcagc gcctgggatc
     1141 tggtttaccc cagcaatttc aactgggaga tccgtactca ccgggtgcgg gtggccaacg
     1201 atctggcctc ccgcgtgtcg ctgctcgtgg cggtggtgcc acggcagacg gaagaagagt
     1261 cggcgcgatt gcgcgaaacc gagcagacgc tcgaggcgcg cggagccgat gcgtccccaa
     1321 gggatcggag caggctctac ctctcgcgcg cctaccagca tcggcggctg ctggagttgc
     1381 ttgcagatac gctggatgcg ctgcagtgcg ttcgatcggc agaagagttg cccagggagg
     1441 ttcactgctg gacccgggcg gccgtcaatc tcatggacga ggagaccgtc gacgtggcgc
     1501 tcacggtgct cagagatgcc cgcatggtgc ctcgggacga ggacatgccg gtgaaggctc
     1561 aggacccgaa ggtctggtac ggcgagtacg gcaagggcat cgtgcggcac atcgtgatgc
     1621 cctatctggc cggtctcgcg gcgcctcgaa cgccggcggc cacggaagag gaagtcagcc
     1681 atgatggttg agcgtgcccc cccgatcatg ttgactttgc tgctctcatc gacgctggcg
     1741 gctgcctcgg agacggtgct cttcaacgag tcggccgcgt tcgagtgcta ccaggcggcc
     1801 ctgcacgacg gcggcgagta tgacatcgag tcgtgcagcc gggctctgga gtatcaggcg
     1861 ctgaccacca tcgacagggc ggccacccat tccaaccgcg gcattctcta cgcccgcatc
     1921 ggcgacatcc gcaacgctct tcgggaccac aaccgcgcgg tgaagctcgc cccggaggtg
     1981 ggcagcatct acatcaaccg ttccaacacc ctggtccggg cgaagcgcta cgagggcgcc
     2041 atgcgcgacc tcgagaaggc gatcgagatt gccgacgaca gtctggctta cgcccattac
     2101 aaccgggcgc tgctctttca gaagctcggt gatcttcagg cggcgagggc ggatgccgag
     2161 caagccgcgg agatagcgcc ggactcaccg gcgtaccggc gcttccttgc tgccttcggc
     2221 gaaaccgagg gcgacccgct cctcccggat gccgagcctg gcaatcctcc ggaggacccg
     2281 ggcggtgagg gtcaggactg aatcaggccc ttgagccggc gcgcggtttc ctcgggcgag
     2341 cccgttcgcc gggcgagcag atgatagaaa gccggaacga tgaagagtgt cagcagcgtt
     2401 gccagggaga cgccggagaa aatgacgacc ccgagcatgc tgcggctcgt cgagccggca
     2461 ccggtcgcga ggatcagcgg cgtcgatccc atgaccgtgg agatggtcgt catgatgacg
     2521 gggcgcaggc gaatgcccgc tgcttccacg atggcgtcgt cgaaagaacg gcctgcatcg
     2581 cgcagctgat tgatgaactc gacgataaga acgccgttct tggcggcaat gccgatgagc
     2641 atgacgatgc cgatctggct gtagatgttg agcgtgccgc cggtcagctt gaggccgagc
     2701 agcgcgccgg caatcgccat ggggacggtc agcaggatga ccagcgggtg gacgaagctc
     2761 tcgaattggg cggccagcac cagatagacg atgagcagcg cgatggcgaa cgtgaacaga
     2821 agtgatccgc tggcttcctt gaactccagc gattcgcccc ggtagtccac ctgggcggat
     2881 tccggcagct cctcacgcac tacggtctcc agaaactcca gagcgtccgc cagggcgtag
     2941 cctggggcca aaccggcctc gatggtcacg gcgcggagcc ggttgtagcg attgaggcgc
     3001 ccagctccgg cggtctcctc cacctcgatg aggttggcca gcggaaccag acggccggtg
     3061 ctttccgagc gaacgtagat gttctgaaga tctgcggccg tcgcccgctg atcctccttg
     3121 gcctgcagga tcacgtcgta ctcttcgccg tcctgcacga aggtcgtgac cctgcgttcg
     3181 ctcatcatgg ccgcaagggt tctgcctacg ttctgcacgg agatgcccag cgccgccgcg
     3241 cgattcttgt cgacccgcac gatcagctgc ggctgggttt ccttcaggtc gctgtcgacg
     3301 cgcgtcatgc cggggtactc catggcgcgt gcgattagaa gatcgcgcca ttgggccagg
     3361 gtctcgtagt tgggcccgcc gatgacgaac tgaaccgggc ggccaccgcc gcctcgggac
     3421 agaccggagc gctcgaaggc gaagatccgc acggcgggaa tttcctccca cgctgtggag
     3481 gcccggtcga tgatctcgcg tgtggtggcg tcccgttccc gccacggctc cagggagacg
     3541 atcaccatgc cggtgttcgg gctcgttccg ccccagctgg ggacccggac cagcactctc
     3601 gagatcaggc cctcgtcctg catctgcatc aagggcgcct cgacgtcgcc catgtgttcc
     3661 tg
//
LOCUS       DBNHQR010000032         2033 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_763651, whole genome shotgun sequence.
ACCESSION   DBNHQR010000032 DBNHQR010000000
VERSION     DBNHQR010000032.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_763651"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="AC16VR_00740"
     CDS             <1..594
                     /locus_tag="AC16VR_00740"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6009704.1"
                     /translation="GRFFGEVGVDPNSGMEGVRALETCVGLHPNIKAASAAPCLLNPQ
                     VPIDDKKFYPIYAKCCELDIPINMLVGVPGPRVPYRCQHPGLLDEVAWFFPELKVVMR
                     HGGDPWTDLCVKLLLKWPNLYYSTSAWAPKHYPRNVLEFANKRGRGKVLYAGYFPGLS
                     YERIFTEMDDLPLTDDTWPAFLRDNAINVYKLQDILE"
     gene            591..1769
                     /locus_tag="AC16VR_00745"
     CDS             591..1769
                     /locus_tag="AC16VR_00745"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015270460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6009705.1"
                     /translation="MNQPDTALSPKVSELVARTTEFVTDKVIPYEKDPRNGSHGPSDA
                     LRVELQTAAREAGLLVPQLAEHLGGHDLNHREMAAVFRAAGYSPLGPIALNIMAPDEG
                     NMHLLHKVATPEQQERFLQPLAAGTIRSAFLMTEPDGGAGSDPGMLATMAEREGDDWV
                     INGRKSFITGGEGAGFGIIMAKTGSSATMFLADMNTPGISIERVLDTIDSSMPGGHAV
                     VRLDGVRVPADQILGELDEGFRYAQVRLAPARLTHCMRWWGSAQRAHDIASDYACRRQ
                     AFGKPIIDHEGVGFMLADNLMDLKQAELMIDWCAGVLDSGAPGTAESSMAKVAVSEAL
                     FRVADRCVQVLGGTGVTQDTIVEQVFREIRAFRIYDGPSEVHRWSLAKRIKRERLAST
                     "
     gene            complement(1793..>2033)
                     /locus_tag="AC16VR_00750"
     CDS             complement(1793..>2033)
                     /locus_tag="AC16VR_00750"
                     /inference="COORDINATES: protein motif:HMM:NF039725.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6316 family protein"
                     /protein_id="HIR6009706.1"
                     /translation="RSSRFFQIHGVWFVSTREGINIGPYGTQFEARKHERHLIALLVR
                     ARDPAEARRVIYRFKHRPNLTGTPWSQTDEVARTG"
BASE COUNT          353 a          675 c          660 g          345 t
ORIGIN      
        1 ggccgattct tcggcgaggt aggcgtcgat cccaactcgg gtatggaggg tgttcgcgcg
       61 ctcgagacct gcgtcgggct gcaccccaac atcaaggccg cctccgccgc gccctgcctg
      121 ctgaatcctc aggtgcccat cgacgacaag aagttctatc ccatctacgc gaagtgctgc
      181 gagctcgaca ttcccatcaa catgctggtc ggcgtgccgg gaccacgcgt tccctacagg
      241 tgccagcatc cgggcctgct cgacgaggtg gcctggtttt tcccggagct gaaggtggtc
      301 atgcggcacg gcggggaccc atggacggac ctttgcgtga agctgctgtt gaagtggccg
      361 aatctctact attccacgtc tgcctgggcg cctaagcatt accccaggaa cgttctcgag
      421 ttcgccaaca agcgcggccg cggcaaggtg ctctacgccg gctatttccc cggcctgagc
      481 tacgagcgca tcttcacgga gatggacgac ctgccgctga cagacgatac ctggccggca
      541 tttctccgcg acaacgccat caacgtctac aaacttcagg acatcctcga atgaaccagc
      601 cggacaccgc gttgagcccc aaagtttctg aactggtcgc gcgaaccacc gagttcgtca
      661 ccgacaaagt cattccctac gagaaggatc ctcgcaacgg atcacacggg cccagcgatg
      721 ctctgcgagt cgaactgcag acggcggctc gtgaggccgg cctcctcgtg ccccagttgg
      781 ccgagcacct cggcggccat gacctcaacc accgggagat ggctgccgtg ttccgggccg
      841 ccgggtactc gccgctgggg cccatcgcgc tgaacatcat ggcccccgac gagggcaaca
      901 tgcacctgct gcacaaggtg gcaacccccg agcagcagga gcggttcctg caacctctgg
      961 cggcgggcac catccggtcc gcgttcctga tgaccgagcc cgacggcggc gcggggtcag
     1021 acccgggcat gctggccacc atggcggaga gggagggcga cgactgggtg atcaacggcc
     1081 gcaagtcgtt catcaccggc ggcgaaggcg ccgggttcgg catcatcatg gccaagaccg
     1141 ggtcgtcggc aaccatgttt ctcgccgaca tgaacacgcc gggcatctcc atcgagcgcg
     1201 tcctggacac catcgacagc tccatgcccg ggggccacgc cgtcgtgcgc ctcgacgggg
     1261 tgcgggttcc ggcggaccag atcctgggcg agctggacga gggcttccgc tatgcccagg
     1321 tgcgcctggc gcccgcgcgt ctgacccact gcatgcgctg gtgggggagc gcccagcggg
     1381 cccacgacat cgcctcggac tacgcctgcc ggcgccaggc cttcggcaag ccaatcatcg
     1441 accacgaggg tgtcggcttc atgctggccg acaacctgat ggacctgaaa caggccgagc
     1501 tgatgatcga ctggtgcgcc ggggtgctcg actcgggcgc gccgggcacc gccgagtcga
     1561 gcatggcgaa ggtcgccgtc tccgaggcgc tgttccgtgt cgccgaccgc tgcgtgcagg
     1621 tgctgggcgg caccggggtg acccaggaca ccatcgtgga gcaggttttc agggagatca
     1681 gagcctttcg gatctacgat ggcccctccg aggttcaccg ctggtctctg gccaagcgga
     1741 tcaagcggga gcggctcgca tcgacttgag agctgccgcc gtgaagccgc gttcagccgg
     1801 tgcgggctac ctcgtcggtt tgagaccagg gagtacccgt caggtttggt cggtgcttga
     1861 agcgatagat cacccgtctg gcctccgcgg gatctctcgc cctgacgaga agcgcgatga
     1921 ggtggcgctc gtgcttcctg gcctcgaact gcgttccgta gggcccgatg ttgatgccct
     1981 cccgggtgga cacgaaccac acgccgtgga tctggaagaa acggctcgaa cgg
//
LOCUS       DBNHQR010000033         2479 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1206877, whole genome shotgun sequence.
ACCESSION   DBNHQR010000033 DBNHQR010000000
VERSION     DBNHQR010000033.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1206877"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="AC16VR_00755"
     CDS             complement(<1..622)
                     /locus_tag="AC16VR_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009707.1"
                     /translation="MAEIPEHILRRSAEARAKAEGRTVEEVLAEMKGEAPPAPPAPAA
                     GDTAATPAPAPAAAGPVDMAAAAEAAGIPESLFRRSVEAKAKAQGVSVEAMLAEMTGA
                     PSPAAAAPAEATPAPAPAAAAPAPAPAAGGPARDLAAEEAEYGVPAVLLRRAALARSK
                     WDGTAAPQFAGEAAPPPTPAVAAAAGGAAAAPAAAPAIPEGVRTQRL"
     gene            complement(631..1413)
                     /gene="extP"
                     /locus_tag="AC16VR_00760"
     CDS             complement(631..1413)
                     /gene="extP"
                     /locus_tag="AC16VR_00760"
                     /inference="COORDINATES: protein motif:HMM:NF040969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenite/tellurite reduction operon b-type
                     cytochrome ExtP"
                     /protein_id="HIR6009708.1"
                     /translation="MANGNGNGRVGLKDRLAEAGDHIRQSQVWSSIFRPGSPFRHGYS
                     DSPRNRSYVIMNNVLYHLHPVKVKRHGVRLSYTLCLGGLSFFLFILLTITGIFLMFYY
                     RPTSPQAFQDIQALATDVAFGQLVRNMHRWGAHAMVLTVFLHMARVFYHGAYKPPREF
                     NWVVGVILLLLTLLLSFTGYLLPWDQLALWAVTVGSNMAAYSPVIGDQVTFTLIGGVQ
                     ITPDTLLRWYVLHVLFLPFVIVIFMAVHFWRVRKDGGISGPL"
     gene            complement(1486..1932)
                     /locus_tag="AC16VR_00765"
     CDS             complement(1486..1932)
                     /locus_tag="AC16VR_00765"
                     /inference="COORDINATES: protein motif:HMM:NF024924.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6009709.1"
                     /translation="MAKTDIWFETPPMRDDYQLAAVDGEYLKAAVKPKQFLHIDQSEC
                     ILCEGCVDICPWKCIHYLSIDAIAEGTNVDNPGDSAENQGFFLVDEDECTRCALCIDR
                     CPTGVITLGKFAGSLADQAQEFEQKPWEYNTQQRDFKNGYSYGMRW"
     gene            complement(1936..>2479)
                     /locus_tag="AC16VR_00770"
     CDS             complement(1936..>2479)
                     /locus_tag="AC16VR_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009710.1"
                     /translation="GGLTAIAGSLIAHFTGLPETDALGRDLYPSIPRGWVPVVIGQII
                     SLSGTFVFLAGICFAFVYKRPLTWARAALGAFVFVSVSIILFGIVPNEWLTLTQSVWE
                     WTPQKIAVTIPPWLVLNNEISVSYAAVKDIVSGTYAIVVLGGMAVGMYQWQEYQKKVA
                     SGPPPQPVSTYGRPLSKVER"
BASE COUNT          425 a          771 c          873 g          410 t
ORIGIN      
        1 ggagccgctg cgtccgaacg ccttccggta tggcaggcgc cgccgcgggt gcggccgcgg
       61 caccaccggc cgccgcggct acggccgggg ttggcggcgg tgcggcttcg cctgcgaact
      121 gcggggcggc cgtgccgtcc cacttggacc gggcgagggc ggcgcggcgt agcaatacgg
      181 cgggaacgcc gtattcggct tcctcggcgg cgaggtcgcg agcaggcccg ccggcggccg
      241 gtgccggcgc gggcgcagca gccgcagggg caggggcagg ggtggcttcg gccggtgccg
      301 ccgcggcggg tgacggggcc cccgtcatct cggcgagcat cgcctcgacg ctcactcctt
      361 gcgcctttgc cttcgcctcg accgagcggc ggaacagcga ctcgggaatc ccggcggcct
      421 cggcggcggc agccatgtcg acagggcctg ccgcggcggg tgcgggtgcg ggggtcgccg
      481 ccgtgtcgcc ggcagcgggc gcgggcggag ccggtggcgc ttcgcccttc atctcggcga
      541 gcacctcttc tacggtgcgt ccctctgcct tggcgcgagc ctcggcggaa cgccgcagga
      601 tgtgttcggg tatctctgcc acagcgctcc ttacagcggt ccggagatgc cgccgtcttt
      661 gcgtacccgc cagaagtgca ccgccatgaa gatgacgatg acgaacggca ggaacaagac
      721 atggagaacg taccagcgca gcaacgtgtc cggcgtgatc tggacaccgc caatcagcgt
      781 gaaggtcact tggtcgccga tcacagggct gtacgccgcc atgttggatc caacggtgac
      841 ggcccagagc gcgagctggt cccacggcag caggtagccg gtgaacgaca gcagcagcgt
      901 gagcagcagc aggatgacgc caacgaccca gttgaactca cgcggcggtt tgtaggcgcc
      961 gtgatagaac acgcgggcca tgtgcaagaa gacggtcagc accatggcgt gcgcacccca
     1021 gcggtgcatg ttgcgtacca gctgtccgaa ggcgacgtcg gttgccagcg cttggatgtc
     1081 ctggaacgcc tggggcgatg tgggccggta gtagaacatc aggaagatgc cggtgatggt
     1141 cagaaggatg aagaggaaga agctcagacc gccgagacac agcgtgtagg acagtcgcac
     1201 gccgtgtcgc ttcaccttca cggggtgcag gtgatagagc acgttgttca tgatgacgta
     1261 ggagcggttg cgcgggctgt ctgagtagcc gtggcggaag ggtgaaccgg gacggaagat
     1321 gctcgaccac acctggctct ggcgaatgtg atcgcccgcc tcggcgagac ggtctttcag
     1381 cccgacccgg ccgttcccgt ttccgttggc cacctgtgct ccctcgtcag cttccgcggc
     1441 gccggcagtt tcaccgaacg gcgccgatgg tcaaaattcc ctttcctacc agcgcatgcc
     1501 gtaggagtaa ccgttcttga agtcgcgttg ctgggtgttg tactcccagg gcttctgctc
     1561 gaattcctgc gcctggtcgg cgagcgatcc ggcgaacttg ccgagcgtga tgacgcccgt
     1621 ggggcaccgg tcgatgcaga gcgcacaacg agtgcactcg tcctcgtcga ccaggaagaa
     1681 cccctggttc tcggcgctgt cgcctgggtt gtcgacgttg gtgccctcgg cgatggcatc
     1741 gatcgagagg tagtggatgc acttccaggg gcagatgtca acgcaccctt cgcacagaat
     1801 gcactccgac tggtcgatat ggaggaactg cttgggcttg acggctgcct tgaggtactc
     1861 gccgtcgact gctgcgagct ggtagtcgtc gcgcatgggc ggcgtctcga accagatgtc
     1921 ggtcttggcc acgggttacc gctccacctt ggacagggga cgtccgtacg tcgacacggg
     1981 ctgcggcggc ggtccggacg cgaccttctt ctggtattcc tgccactggt acatgccgac
     2041 cgccatgccg ccgaggacca cgatggcgta cgtgcccgac acgatgtctt tgacggcggc
     2101 gtagctcacc gatatctcat tgttgagcac cagccagggt ggaatcgtga ccgcgatctt
     2161 ctgcggcgtc cactcccaca ccgactgggt gagggtgagc cactcgttcg ggacgatgcc
     2221 gaacaggatt atggacaccg agacgaacac aaacgcaccc agcgccgccc gagcccaggt
     2281 cagcgggcgc ttgtagacga aggcgaaaca gatgccggcg aggaagacga acgtacccga
     2341 gagggagatg atctgaccga tgacgaccgg cacccaacca cgggggatcg aagggtagag
     2401 gtcacggccc agggcatcgg tttcgggcag gcccgtgaag tgggcgatca gggaaccggc
     2461 gatcgcggtc aggcctccc
//
LOCUS       DBNHQR010000034         2215 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_256528, whole genome shotgun sequence.
ACCESSION   DBNHQR010000034 DBNHQR010000000
VERSION     DBNHQR010000034.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_256528"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1428
                     /locus_tag="AC16VR_00775"
     CDS             <1..1428
                     /locus_tag="AC16VR_00775"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6009711.1"
                     /translation="RLLPDPRSGAKPRRVRGIFLALVAVCLQVPVLAVAAEIDFTRHL
                     VRVESAAGQHPGPDVGPARPFFGVAGPARVIVTARSGEPVVTVRLNGVDVLDGQALRQ
                     TTSLEIPVELAEANTIAVGLDGRDGAAASVRVKQRADVALNVISWVHFNTNVSDFNEA
                     RAFYGVLGFETMSGFPDTNTLAMAHALGIQTPTAYDGSQGEEAGGYLLHGELVGPDGF
                     WGGVIDLIEFTIPRNEEPPYPGLNHLGMARALMHTGDIAAAYDQLVGAGVNFLSPPAT
                     RSDGTRFAVLTDPDGTFYELVELEGADGESSDKPVIGLGRVNVNVSDFERSEAWYRMF
                     GYVATRKLPPTDSAEVAAAMGFDAPYRIDGAVLSHEVDGSQIELVQWLSPFDPEPAYP
                     PPVSHLGIHRMALLTGDIEADVAALKAQGVKFISGITPCCSGPDSWGSIVLFYDPDGT
                     VVELVEQPFLGTVARAVIWLRRLFE"
     gene            1615..>2215
                     /locus_tag="AC16VR_00780"
     CDS             1615..>2215
                     /locus_tag="AC16VR_00780"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR6009712.1"
                     /translation="MGRALSLLVPLIWLGIGMPGPALAESDSLALEEIVVTARKREES
                     MQEVGMALSALGAEDLKIRFDADLQTLQNASPNLIVSDIQQGPGSPAAISIRGIGTTD
                     VEKNFDPTVGVVVDGVFIGVNSGAMLKAIDLESVEIMRGPQGTLFGRNSIGGVINVTR
                     ARPDFDGLSGSLRAGAGNHGDLQLDAYINITATENLAFRL"
BASE COUNT          365 a          718 c          741 g          391 t
ORIGIN      
        1 cggcttttgc cggaccctcg ctccggggca aagccgcggc gcgtgcgcgg gatattcctc
       61 gcgctggttg ccgtttgcct gcaggtgccc gtcttggcgg tcgccgctga gatcgacttc
      121 acccgccatc tggtacgggt cgaaagcgcg gccgggcagc accccgggcc cgatgtcggg
      181 ccggcgcggc cgttcttcgg ggttgccggc cctgcccggg ttatcgtaac cgcacgatcc
      241 ggtgagccgg tggtgaccgt ccggctgaac ggcgtcgacg tgctcgacgg gcaggcgtta
      301 cggcaaacga cctccctgga gatcccggta gagcttgccg aggcgaacac cattgccgtt
      361 ggcctcgacg gccgggacgg cgcagcggcg tcggtgcgcg tcaagcagcg cgcggacgtc
      421 gcgctgaacg tcatctcctg ggtgcacttc aacaccaacg tgagcgattt caacgaagcc
      481 cgcgcgtttt acggcgtgct cgggttcgaa accatgagcg gctttccgga caccaacacc
      541 ctggcgatgg cccacgccct gggcatccag acgccgacgg cctacgacgg ctcccaggga
      601 gaagaggccg gcggctacct gctccacggc gagctggtgg gccccgatgg tttctggggc
      661 ggcgtcatcg atctcatcga gttcaccatt ccgagaaacg aagagccgcc gtacccgggc
      721 cttaaccacc tgggcatggc gcgggctctc atgcacacgg gcgacattgc ggccgcctac
      781 gaccagctcg tgggagccgg cgtaaacttt ctctctccgc ctgccacccg ctcggacggt
      841 acccggtttg ccgttctcac cgatcccgac gggacgttct atgagctggt cgaactggaa
      901 ggcgctgatg gcgagtcatc cgacaagccc gtcatcggtc tcggtcgggt gaacgtcaac
      961 gtgagcgact tcgaacgctc cgaagcctgg taccggatgt tcggatacgt agccacccgc
     1021 aagctgcctc cgacggactc cgccgaggtg gcagcggcca tggggttcga tgcgccctac
     1081 aggatcgacg gggccgtgct cagccacgag gtggacggct cgcagatcga gctggtccag
     1141 tggctttcgc ccttcgaccc cgagcccgcc tacccgccgc cggtcagcca cctgggcatt
     1201 catcgcatgg cgctgctcac cggcgacatc gaggccgacg tggctgcgct caaggcccag
     1261 ggtgttaagt tcatctccgg gatcaccccg tgctgctcgg ggccggattc gtggggaagc
     1321 atcgtcctgt tttacgatcc ggacgggacg gtggtggagc tggtggagca gcccttcctg
     1381 ggcacggtgg cccgtgcagt catctggttg cggcgactgt tcgagtagcc gacgcaggtt
     1441 ttctgccgcc gagcgagtgg tttcggcgcg ctcagcctca agtctataat ctgccactgc
     1501 ccgccggtgg gcgggaccaa ggggaacgag cgttcgcggg tagccgctgc gcggtgtgtt
     1561 cgaataacag aatcggaaac tagcctactg gagagaatca tgaggagagc aatcttggga
     1621 cgagcgctgt cgctgctcgt accgttaatc tggctcggca tcggtatgcc tgggcccgcg
     1681 ctggccgagt ccgactcgct tgccctggag gagatcgtcg tcaccgcgcg gaagcgcgag
     1741 gagagcatgc aggaagtggg gatggccctg agcgcgctcg gcgctgagga tctgaagatc
     1801 cgcttcgacg cggacctgca gaccctgcag aacgcctcgc ccaacctgat cgtcagcgac
     1861 attcagcagg gtccgggcag ccccgcggcc atctcgattc gcggcatcgg caccacggac
     1921 gtggagaaga acttcgatcc caccgtgggc gtcgtggtcg acggcgtttt catcggggtg
     1981 aactcgggtg caatgctgaa ggccatcgac ctggaaagcg tggagatcat gcgcggaccc
     2041 cagggcacgc tgttcgggcg caactccatc ggcggcgtca tcaacgtgac ccgcgcgcgg
     2101 ccggacttcg acggcctgtc cgggtcgctc agagccggtg caggcaacca cggcgacctg
     2161 cagctcgatg cttacatcaa catcacggct acggaaaatc tcgccttccg tcttg
//
LOCUS       DBNHQR010000035         3610 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1523500, whole genome shotgun sequence.
ACCESSION   DBNHQR010000035 DBNHQR010000000
VERSION     DBNHQR010000035.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3610)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1523500"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="AC16VR_00785"
     CDS             complement(<1..675)
                     /locus_tag="AC16VR_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007350045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6009713.1"
                     /translation="MSSEGPLHGIRVVELGQLIAGPFCGQLLGDFGAEVIKVEPPGAG
                     DPMRGWGQGAPVWWPVIARNKKSVTLNLREAEGQELLKQLAVKADVLIENFRPGTMEK
                     WGLDYGTLAALNPGLIMVRVSGFGQTGPYAHRAGYGSIGEAMGGIRYLAGDPDRPPSR
                     VGLSIGDSLAATFACLGTLAALHSRHATGRGQVVDSAIYEAVLAMMESTIPEFTEAGV
                     IRERSGA"
     gene            complement(665..1600)
                     /locus_tag="AC16VR_00790"
     CDS             complement(665..1600)
                     /locus_tag="AC16VR_00790"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HIR6009714.1"
                     /translation="MSEADQRAVTVMEVSPRDGLQNEAEVLSTQNKLALIDLALEAGC
                     RRLEAVSFAHPARVPQMADAEAVCLGLPQRGDVIYTGLVMNQRGYERLLATERLDEAG
                     LVVPATDTFGQRNQGMDVAANVAMARSVLRDARLRGLRAQATIAVAFGCPFEGEVPVQ
                     RVVAIASAIAEEGPVEIGLADTVGVGVPGQVLEAFSAVAEAVGPNIPLRAHFHDTRNT
                     GIANAFAALQAGVATLDASIGGIGGCPFAPNATGNIATEDLVYMLDRMGVDHGLSLER
                     LLAGAEWLQGVLGRPVPSSLLRAGGFPQPEPVDVV"
     gene            complement(1646..3283)
                     /locus_tag="AC16VR_00795"
     CDS             complement(1646..3283)
                     /locus_tag="AC16VR_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HIR6009715.1"
                     /translation="MAEEPNDFIRQRIRADLASGVVKGGVVTRFPPEPNGHLHIGHAK
                     SICLNFGVAEEFGGYTYLRFDDTNPLKESEEFALAIQEDVRWLGYDWGDHLTHASDYF
                     EELYGFAAQLIEQGKAYVCSLTGDEIRATRGSLTEPGTESPYRDRSVEENLNLFRRMR
                     EGEFADGEHVLRAKIDMASPNINMRDPVIYRIRHASHQRTGDAWVIYPMYDFTHCICD
                     ALEGITHSLCTLEFEDHRPLYDWVLDNININFHPPQIEFSRLGLEYTVMSKRLLHRLV
                     AEGLVEGWDDPRMPTIAGLRRRGVTPGAIREFCRRIGVTKQDGMVEMNLLEFCIRQDL
                     ESSAPRGMAVLDPLKVVITNHPGAEEYLVAPWHPNEAELGERELPFGPEIYIERADFM
                     EEPPRKYKRLSPNEMVRLRYAYVIRCDEVVRDEVGNIAELRCTYFSDSKSGADTSGLK
                     PKGVIHWVSAATGMPTRIRLYDRLFTVPAPRGEELVGELNTQSLAEMSGFVEPAVAAS
                     AARRFQFERQGYFCKDSQLDGVFNRTVTLRDSWKPAP"
     gene            3381..>3610
                     /locus_tag="AC16VR_00800"
     CDS             3381..>3610
                     /locus_tag="AC16VR_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009716.1"
                     /translation="MTLRLIVAGAICIALGAGSVLLEQHASQAVQSARHFLGERWYVL
                     ELGGRQVGYLHTGASHDREGQWQFRSELRFAL"
BASE COUNT          621 a         1225 c         1145 g          619 t
ORIGIN      
        1 ggcgccgctg cgctcacgga tgacgccggc ttcggtgaac tcgggaatcg tcgactccat
       61 catggccagc acggcttcgt agatggcgga atccaccacc tgccctcgtc cggtcgcatg
      121 ccggctgtgg agggccgcca gggtgcccag gcaggcgaag gtggctgcca gggagtcgcc
      181 gatggaaagc cccacgcggc tcggcggccg gtccgggtca cccgccagat agcgtatacc
      241 gcccatggcc tcgccgatcg agccgtagcc cgcccggtga gcgtatgggc ccgtctgtcc
      301 gaatcccgac accctgacca tgatgaggcc cggattcagc gcggcaagag tgccgtagtc
      361 caggccccac ttctccatgg tgccgggccg gaagttctcg atcagcacgt ccgccttgac
      421 cgcgagctgc ttgagcagct cctgcccttc tgcctcgcgc aggttcagcg tcaccgattt
      481 cttgttgcgc gcgataaccg gccaccagac gggcgcgccc tggccccacc cgcgcatggg
      541 atcccccgca ccgggaggtt ccaccttgat cacttccgca ccgaagtcac ccagcagctg
      601 accgcagaaa ggcccggcaa tcagctggcc cagctcgacg acgcgtatgc cgtgcagggg
      661 cccttcagac gacatcgacg ggctcgggct gcggaaaacc gccggcacgc agaaggctcg
      721 acggcaccgg acgaccaagc accccctgca accattccgc cccggccagc aggcgttcga
      781 gggacaaccc gtggtctacg cccatgcgat cgagcatgta aacgagatcc tcggtggcga
      841 tgttgccggt ggcgttgggg gcgaaggggc aaccgccgat accgccgatg ctggcgtcca
      901 gggtggcaac ccccgcctgc agggcggcga aggcgttggc aatgccggtg ttacgggtgt
      961 cgtgaaagtg ggccctcaac gggatgttcg ggccgacggc ttctgccacc gcgctgaacg
     1021 cctcgagcac ctgaccggga acgccgaccc ccaccgtgtc ggccaggccg atctccaccg
     1081 gtccctcttc ggcaatggcc gacgcgatcg ccacgacgcg ctggacgggc acctcgccct
     1141 cgaaggggca accgaacgcc acggcaatgg tcgcctgggc gcgcagccct ctcaaacgcg
     1201 cgtcccgcag caccgaacgg gccatggcga cgttggcggc aacgtccatg ccctgattgc
     1261 gctggccgaa cgtgtcggtg gccggaacga ccagcccggc ctcgtcgaga cgctcagtgg
     1321 ccaaaagacg ctcgtagccg cgctggttca tcacgagacc ggtgtagatc acgtctccac
     1381 gctgcggcag gcccaggcac acggcttccg catcggccat ctgtggaacc cgagcagggt
     1441 gggcaaagct gaccgcctcc agccggcgac agcccgcctc gagggccagg tcgatgaggg
     1501 ccagcttgtt ctgcgtgctg agcacctcgg cttcgttctg cagcccgtcg cgggggctca
     1561 cctccatgac ggtaacggcc cgctggtcgg cttctgacat ttacgtgacc tcctggttat
     1621 gccctgaacg gaccaccgcg acggcctaag gagcgggctt ccagctgtcc cggagcgtca
     1681 ccgtgcggtt gaaaacccca tcgagctgag agtccttgca gaaatagccc tgtcgctcga
     1741 actgaaacct tctcgcggcg ctcgccgcca ccgccggttc aacgaaaccg gacatctcgg
     1801 ccagcgactg cgtattgagc tcgccgacga gctcctcgcc acgcggcgcg gggacggtga
     1861 agagccgatc gtacagcctg attcgtgttg gcatgcccgt ggccgccgat acccagtgaa
     1921 tgacgccttt gggcttgagg ccgctggtgt ccgcgccgct cttcgagtcg gagaaatagg
     1981 tgcagcgcag ctcggcgatg tttcccacct cgtccctgac cacctcgtcg caccggatca
     2041 cgtaggcgta gcgcaggcgc accatctcgt tgggagacag acgcttgtac ttcctcggcg
     2101 gttcctccat gaagtcggcg cgctcgatgt atatctccgg gccgaacggc agctcgcgct
     2161 cgcccaactc tgcctcgttg ggatgccacg gcgccaccag atactcctcg gcgcccgggt
     2221 ggttggtgat caccaccttg agcgggtcca aaacggccat gcctcggggc gcggagcttt
     2281 ccagatcctg tcggatgcag aactccagca ggttcatctc caccataccg tcctgcttgg
     2341 tcaccccgat acggcggcag aactcgcgaa ttgctccagg ggtcacccca cgacgcctga
     2401 gacccgcgat ggtcggcatg cgcgggtcgt cccagccttc caccagccct tccgccacca
     2461 ggcggtgcag gagacgcttg ctcatgaccg tgtactcgag ccccaggcgg gaaaactcga
     2521 tctgcggcgg gtgaaagttg atgttgatgt tgtccagcac ccagtcgtag aggggccggt
     2581 ggtcctcgaa ttccagcgtg caaagcgagt gcgtgatccc ttccagggca tcgcagatgc
     2641 agtgcgtgaa gtcgtacatc gggtagatca cccaggcatc acccgttcgc tgatgagaag
     2701 catggcggat ccggtagatc acgggatcgc gcatgttgat gttcggggac gccatgtcga
     2761 tcttcgcccg cagaacgtgc tcaccgtccg caaactcgcc ttcgcgcatg cgccggaaga
     2821 gattgaggtt ctcctccacg gatcggtcgc gatagggact ctcggtgccg ggctcggtga
     2881 gggacccccg ggtcgcccgg atctcgtcac cggtcaggct gcagacgtag gccttgccct
     2941 gctcgatcag ctgcgcggca aagccgtaga gctcctcgaa atagtccgag gcgtgggtga
     3001 ggtggtcgcc ccagtcgtaa cccagccagc gcacgtcctc ctgaatggcc agggcgaact
     3061 cctcgctctc cttgagcggg ttggtgtcgt cgaagcgaag ataggtgtag cccccgaact
     3121 cctcggcaac cccgaagttc aggcagatgg acttggcgtg gccgatgtgc agatgcccgt
     3181 tcggctccgg cggaaatcga gtcaccacgc cgcccttcac gacgccgctg gcaaggtccg
     3241 cgcgaatgcg ctggcgtatg aagtcgttgg gttcttcggc catcggccca tctacggaat
     3301 ctgcggggcg cgtattctaa ccaaaggccg aaaccggcgt ctgctgctaa tattcttcgg
     3361 gaaagcacag ggggagcttc gtgactttac ggttgatcgt cgccggcgcg atctgcatcg
     3421 cgctcggcgc gggatcggtc ctgctcgagc aacatgcctc ccaagccgtc cagagcgccc
     3481 gtcacttcct cggtgaacgc tggtacgtcc tggagctcgg cggtcgccag gtcggctacc
     3541 tgcacaccgg cgccagccac gatcgcgagg gccaatggca gttccgcagc gagctgcgat
     3601 tcgcgctgca
//
LOCUS       DBNHQR010000036         1183 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_446863, whole genome shotgun sequence.
ACCESSION   DBNHQR010000036 DBNHQR010000000
VERSION     DBNHQR010000036.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_446863"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..936
                     /locus_tag="AC16VR_00805"
     CDS             85..936
                     /locus_tag="AC16VR_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009717.1"
                     /translation="MSLAFSLVALWVATIRPPVEQRPEPAAGDAGRVASRAPAVTAQP
                     PVDERAEMIRQTAAPDEKNQADPGFDPMLVTGLGDFNAEEIERYNRLHLLPFNPAIGR
                     DCEDRPDPQLPGRSYTSCTTVRERPAHPYSELGDEVLLSLAEHDAAAALMLGRRVAGE
                     DERLYWYLRAAALSEKSGPLMALAEQRYRDSMDLRLVRGELRRVPRPDRIAVRVALDT
                     VAGRMGDPRANPDRWRGVLRSLAADRAPTYLAQADALVAEFLGAMARTQREITGSVQI
                     QEIIDNA"
     gene            929..>1183
                     /locus_tag="AC16VR_00810"
     CDS             929..>1183
                     /locus_tag="AC16VR_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009718.1"
                     /translation="MRRRLLMAAALVFPGMAVAEALRTECAGCETLRDFGNYGAAQLY
                     RAVGPMGPAVLNDRIWVVNPVTGLQAFVDLDTPTTLVYLFG"
BASE COUNT          195 a          385 c          391 g          212 t
ORIGIN      
        1 ccgggcgctg agctcgcggg acgatgattt gccccatgga catggtaacg caagaggtcc
       61 cgcatgatgc gaatcgtcct gatcttgagt ctggctttca gtctcgtcgc gctatgggtg
      121 gcgaccatcc gtccgcctgt cgagcaacgt cctgaacccg ccgccggcga tgccgggcgg
      181 gtggcgtccc gggcgccggc ggtgacggcc cagcctcctg tcgatgaacg agcggaaatg
      241 attcggcaga cggctgctcc cgatgagaag aaccaggccg atccgggttt cgatccgatg
      301 ctggtgacgg gtctcggtga cttcaacgcc gaagagatcg aacggtataa ccgccttcac
      361 ctgctcccat tcaatccagc catcgggcgt gattgcgagg atcgtcccga cccgcagttg
      421 cccgggcgct cctacaccag ttgcacaacc gtccgcgaac gacccgctca cccttacagc
      481 gagctgggcg acgaggtgct gcttagcctc gccgagcacg atgctgccgc agccctgatg
      541 cttggccgcc gcgtcgccgg agaagacgag cgcctttact ggtacctgcg tgctgcagcg
      601 ctgtcagaaa agagcgggcc gctcatggcc ctggccgagc agcgttaccg tgactcgatg
      661 gaccttcggc tcgtgcgagg ggagttgcgc cgcgttcccc gacccgaccg catagccgtt
      721 cgcgttgccc tggacacggt ggctgggcgc atgggagacc ccagggcgaa cccggaccgg
      781 tggcgagggg tgctgcgctc ccttgccgcc gatagagcgc caacctatct cgcccaggcc
      841 gatgccctgg tggccgagtt tctcggcgcc atggcgcgaa cccagcgcga gatcacgggc
      901 agcgtgcaga tccaggagat catcgacaat gcgtagacgt ctcctgatgg ccgctgcgct
      961 tgtgttccct ggaatggctg tcgccgaggc cctgcgcacc gaatgcgcgg gatgcgaaac
     1021 gctgcgcgac ttcggtaact acggcgcggc gcagctgtat cgcgccgtag gccccatggg
     1081 cccggccgtt ctgaacgatc ggatctgggt agtcaacccg gtgaccggcc tgcaggcgtt
     1141 cgtagatctg gatacgccga cgacgctggt ctatctgttt ggg
//
LOCUS       DBNHQR010000037         5251 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1587257, whole genome shotgun sequence.
ACCESSION   DBNHQR010000037 DBNHQR010000000
VERSION     DBNHQR010000037.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1587257"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /gene="trpS"
                     /locus_tag="AC16VR_00815"
     CDS             complement(<1..947)
                     /gene="trpS"
                     /locus_tag="AC16VR_00815"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014871007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIR6009719.1"
                     /translation="MRPTGRLHLGQYHGVIRNWLELQHGYECFFFVADWHALTTGYED
                     TGNIEQNTFDMVIDWLAAGVNPGTATVFVQSWVPEHAELHLLLSMITPVSWLERVPSY
                     KDQQARLNDRDLSTYGFLGYPVLQSADILMYRAGNVPVGADQVAHVELTREIARRFNH
                     VYGREPGFEDLAIAAIGKMGKKAARLYKSLRRGYVEQGDGEALERARALLAEQSNLSI
                     GDRERLFGYLEGGGRIILPEPQALLNKAAKVPGLDGEKMSKSYQNTIAMREAPDEVEK
                     KVRTMITDPARVRRDDPGDPANCPVFALHEIYSDAETRA"
     gene            complement(1050..1661)
                     /locus_tag="AC16VR_00820"
     CDS             complement(1050..1661)
                     /locus_tag="AC16VR_00820"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR6009720.1"
                     /translation="MSQYFVVHPSHPQRRLLVTAAQIISGGGLVVYPTDTAYALGCHI
                     GDKQSLESIRRIRRLDKRHHFTLTCRDLSEISLYARVSDANYRILKHLTPGPFTFLLP
                     ATREVPRRLVHPKRRTIGLRIPNNVIALELLAVLGEPMMTTTMRLADQDEPFSDPEQI
                     RESLEHEVDLIIDGGPGGVVPTTVVDLTGAVPEVIRQGLGVFE"
     gene            1761..2321
                     /locus_tag="AC16VR_00825"
     CDS             1761..2321
                     /locus_tag="AC16VR_00825"
                     /inference="COORDINATES: protein motif:HMM:NF016189.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane-spanning protein YciB"
                     /protein_id="HIR6009721.1"
                     /translation="MNQLLDFIPIVLFVAVFFLADIYYATAALMIAVTLQVLAYRLLK
                     KPIGRELALTFWASVIFGGLTLVFRNELFIQWKPTIVNWLLCASLLGSHFVGQRNLME
                     RMLGHQLTLVDQVWVRLNFGWAFGFFLAGALNLVVAYNFSMEFWVSYKLIGGFGLTLV
                     YIVITLVYLSKKGFLTDKPEGLGIEK"
     gene            2311..2946
                     /locus_tag="AC16VR_00830"
     CDS             2311..2946
                     /locus_tag="AC16VR_00830"
                     /inference="COORDINATES: protein motif:HMM:TIGR04211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HIR6009722.1"
                     /translation="MKSKSCLATLLMLAGLTLSARAETVYISDTFAVPLRSGPSNSHR
                     ILHRGLPSGTELTVLERDDEGQFARVRTTRGTEGWIPQQYLVAEPIARDRLDEASREI
                     ERLSSLVAEREAEIAQLRQAGARTWEEIEAGAQPGNRGPAGASAASPGERRLMELNER
                     LRHEFERLAAERDELKSSARQRWLLIGAGIMLAGIAIGVILKARPRRSGWS"
     gene            2950..3720
                     /locus_tag="AC16VR_00835"
     CDS             2950..3720
                     /locus_tag="AC16VR_00835"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF003626.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HIR6009723.1"
                     /translation="MAVLSVNLNKVALLRNARGADQPSVTGAAKTVLAAGAHGITVHP
                     RPDQRHVRPDDVGDLADLLRAYPGIEYNIEGNPFAGPRDNGYPGFDALIESARPHQAT
                     LVPDTDNQLTSDHGWDLTGDNGRLSDHINRYQAAGARVSLFMDPVPEHIARARDVGAD
                     RIELYTGPFAQTVAEFGLDHEESLASLDRYQKAAACAAELGLGVNAGHDLDTHNLRLF
                     RQIEQVAEVSIGHALISDALEVGLTAAVRAYLDVLGHH"
     gene            3796..4317
                     /locus_tag="AC16VR_00840"
     CDS             3796..4317
                     /locus_tag="AC16VR_00840"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR6009724.1"
                     /translation="MAGPTAAQESGLPRVVVQTSVGSFEVELYPDRAPASVANFLSLV
                     DEGFYDGLIFHRVIANFVVQTGGYDADMSYREPPRTVVNESANGLKNQRGYLAMARLN
                     DPDSADAQFYINVRDNTHLDGKPGSPGYTVFGKVISGWEIVVEIELSDTVVRAGMVGV
                     PEVPIVIRRATRG"
     gene            4393..4899
                     /locus_tag="AC16VR_00845"
     CDS             4393..4899
                     /locus_tag="AC16VR_00845"
                     /inference="COORDINATES: protein motif:HMM:NF012534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR6009725.1"
                     /translation="MRTIVVAFATAIFFAAVIVELTDRLWPGSQVALLIVATIGLFLS
                     NLLALRLSGSPAAAAPAKRRSEAPGKQGQQKRPAHQEKRPEPGTARSAAGPREPGKVK
                     WFNRNKGFGFIIRESGEEIFVHQRSIRPVGEGDNRRRPSLRDGQAVTFQVAERDKGIQ
                     AEDVVAED"
     gene            4907..>5251
                     /locus_tag="AC16VR_00850"
     CDS             4907..>5251
                     /locus_tag="AC16VR_00850"
                     /inference="COORDINATES: protein motif:HMM:NF015450.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009726.1"
                     /translation="MNVRALIGERIEAALAETGNPGAPALVQPASRPEFGDYQANGVM
                     GAAKRSGTKPRELADAVLKAAALDDVAAGVEVAGPGFLNITLADDFLSAQLLPEDRMS
                     AVEHPQTVVVDYS"
BASE COUNT          945 a         1733 c         1666 g          907 t
ORIGIN      
        1 caggcgcggg tctcggcgtc cgagtatatc tcgtgcagcg cgaatacggg acagttcgcc
       61 ggatcgcccg gatcgtcacg gcgaacgcgc gccggatcgg taatcatggt gcggaccttc
      121 ttttccacct cgtcgggagc ttccctcatg gcgatcgtgt tctggtagga cttggacatc
      181 ttctccccgt ccagcccggg caccttcgcc gccttgttca acagggcctg aggctcggga
      241 aggatgatac gcccaccacc ttcgaggtag ccgaagagcc gttcgcgatc gccaatggac
      301 aggtttgact gctccgccag cagggctcgc gctcgctcga gggcctcgcc gtccccctgc
      361 tcgacgtatc ctcgtcgcag gcttttgtag agccgcgcgg ccttcttgcc catcttgcca
      421 atggccgcta tcgcgaggtc ttcgaacccc ggctcccgcc cgtagacgtg attgaagcgg
      481 cgggcgatct cccgggtcag ctcgacgtgt gccacctgat cggcacccac gggcacgttg
      541 cccgctctgt acatcagtat gtccgcagac tggagaacgg ggtacccgag aaagccgtag
      601 gtcgaaaggt cgcggtcgtt caggcgggcc tgctggtcct tgtagctcgg cacccgctcg
      661 agccagctca ccggggtgat catcgacagc agcagatgca gctcggcgtg ctcgggcacc
      721 caggactgca cgaagacggt ggccgtaccc gggttgacac ccgcagcgag ccagtcgatc
      781 accatgtcga aggtgttctg ctcgatgtta ccggtgtctt cgtagccggt agtcaacgcg
      841 tgccagtccg ccacgaagaa gaaacattcg tagccgtgct gcagctcgag ccagttcctg
      901 atgacgccgt gatactggcc gaggtgcagc cggccggtcg gacgcatccc cgagagtacc
      961 cggtgactcg actcgttact gctcaacgca gcctctcaac tacaaactga ggcggcatta
     1021 tagggctggg gcccggcaaa accacccgtt cactcgaaca cgcccaagcc ctgccgtatc
     1081 acctccggca ccgctccggt gaggtcgacg actgtggtgg gcaccacgcc gcccgggccg
     1141 ccgtcgatga tgaggtccac ctcgtgctcc aggctctccc ggatctgctc cggatccgaa
     1201 aagggctcgt cctgatcggc aagccgcatg gtggtcgtca tcatgggctc gccgagcacg
     1261 gcgagcagtt cgagcgcgat gacgttgttc gggattctca ggccgatcgt gcgccgcttc
     1321 gggtgaacca gccgacgtgg gacctcccgg gtcgccggca ggaggaacgt gaacggtccc
     1381 ggggtgaggt gcttgagaat gcggtagttc gcatccgaaa cgcgggcgta cagcgagatc
     1441 tccgagagat cccggcaggt cagggtgaag tgatgacgct tgtccaggcg gcggattcga
     1501 cgaatgctct ccagcgactg cttgtcgccg atgtggcatc cgagcgcgta ggccgtatcg
     1561 gtcggataga cgaccagccc gccccccgag atgatctgag ccgcggtgac gagaagccgc
     1621 cgctgcgggt gcgaaggatg cacgacgaaa tactgactca cagacccctg cttaccatgc
     1681 ccactcggtt tctcgtatta tcacacgctt agcgttatcc aggatccccg cgcgcagcgg
     1741 ggctattcgg ccaatcaacg atgaatcagc tgctcgactt catccccatc gtgctgttcg
     1801 tggccgtttt tttccttgcc gacatctact acgccacggc agcgctcatg atcgccgtga
     1861 ccctgcaggt tctcgcctat cgccttttga agaaacccat tggccgcgag ctggcgctga
     1921 ccttctgggc gagcgtcata ttcggcgggc tcaccctggt tttccgcaac gagctgttca
     1981 ttcagtggaa gccgaccatc gtcaactggc ttctgtgcgc atcgctgctg gggagccact
     2041 tcgtggggca gcgcaacctc atggagcgca tgctcggtca ccagctgacc ttggtggatc
     2101 aggtgtgggt gagactcaac ttcggctggg cgttcggctt ctttctcgcc ggcgcgctca
     2161 acctcgtggt ggcgtacaac ttctccatgg agttctgggt gagctacaag ctgatcgggg
     2221 gtttcggcct cacgctcgtc tacatcgtca tcacgctggt atacctgagc aagaaaggtt
     2281 ttctcacgga taagccagag ggccttggca ttgaaaagta aatcctgcct ggcaaccctg
     2341 ctcatgctcg ccggcctcac cctgtcggcc cgggccgaga cggtctacat ctccgacacc
     2401 ttcgcggtcc cgcttcgaag cggaccgtcg aactcgcatc ggatactcca ccgggggctc
     2461 cccagcggaa cggagctgac cgtgctggaa cgggacgacg agggccagtt cgcccgggtg
     2521 cggaccaccc gaggaacgga aggctggatc ccgcagcaat acctcgttgc cgagcccatc
     2581 gcgcgggatc ggctcgacga ggcgagccga gagatcgagc ggctgagcag tctcgtcgcg
     2641 gagcgggaag ccgagattgc ccagctgcgc caggcagggg cgcgaacctg ggaggagatc
     2701 gaggccggcg cccagcccgg caaccgcggc cctgcaggcg caagcgctgc cagccccggc
     2761 gagcggcgcc tgatggagtt gaacgagcgt ctgcgccacg agttcgaacg gctggccgcc
     2821 gagcgtgacg agctgaagag cagcgcgcgc cagcgctggc tgctgatcgg agccggcatc
     2881 atgctggcag gcatcgccat cggggtgatc ctcaaagccc gtccccgtcg cagcggctgg
     2941 agctgatcct tggcggttct gagcgtaaac ctgaacaagg tggccctgct gcgcaacgcc
     3001 cgcggtgctg accagcccag cgtcaccgga gcggcgaaga cggtgcttgc cgcgggcgcc
     3061 catggcatca ccgtgcaccc acgcccggac cagcgtcacg tgcgccccga cgacgtgggc
     3121 gacctggctg acctgctgcg ggcatacccc ggtatcgagt acaacatcga gggcaacccc
     3181 ttcgccggtc cccgagacaa cggctatcca gggtttgatg cgctcatcga aagtgcccgg
     3241 ccccaccagg cgaccctggt gcccgacacc gacaaccagc tgacctccga ccacgggtgg
     3301 gatctcaccg gcgacaacgg ccgactatcc gaccacatca atcgctacca ggccgctggc
     3361 gcccgggtaa gtctcttcat ggacccggtg ccagagcata tcgcccgcgc ccgcgacgtc
     3421 ggggcggacc gcatcgagct ctacaccggt ccgttcgcac agaccgtggc agagttcggg
     3481 ctggatcatg aggaaagcct cgccagcctg gacaggtatc agaaagcggc ggcctgtgcc
     3541 gccgagctcg ggctcggcgt caacgccggg cacgacctcg acacccacaa cctgcggctg
     3601 ttccgacaga tcgagcaggt cgcggaggtc tccataggac acgcgctgat aagcgatgcc
     3661 ctggaagtgg ggctcacggc agccgtgcgc gcttatctgg acgtcctcgg acaccactga
     3721 cctgccggac gattgggcat catgagcaac atcgcacgac tcctgactac cgccatattg
     3781 atcggcgtcg ctgccttggc cggtccaacc gcggcgcaag agagcgggct gccgagagtc
     3841 gtcgttcaga ccagcgtggg cagcttcgag gtagagctct atccggaccg cgctcccgcc
     3901 tcggtagcca acttcctctc actcgtcgac gagggtttct acgacgggct gatcttccac
     3961 cgggtgatcg cgaacttcgt cgtccagacc ggcggctacg acgcagacat gagctaccgg
     4021 gaaccgccgc gaacggtggt caacgagtcc gccaacggtc tgaagaatca acgcggctac
     4081 ctcgccatgg cacgcttgaa cgaccctgat tccgccgatg ctcagttcta catcaacgta
     4141 cgggacaata cccacctcga tgggaagcca ggttcgcccg gctacacggt gttcggcaag
     4201 gtcatctccg gctgggagat cgtggtagag atcgagctct ccgatacggt ggttcgcgcc
     4261 ggcatggtcg gcgtgcccga ggtgcccatc gtcatccgca gggcgacgcg aggctaaccc
     4321 cagcaagcaa ttgcacatcc gcttgggtat gattcaccgt tttttcgctt cagcgctgcc
     4381 tggacaccgc ttatgagaac catcgtcgtc gcgttcgcca ccgccatctt ctttgccgcc
     4441 gtgatcgtcg agctgaccga tcgcctgtgg cctggcagcc aggtggcgct gctcatcgtt
     4501 gccaccatcg gcctgttcct gagtaacctg ctggcgctgc ggctgtccgg cagcccggca
     4561 gcggcggccc ctgcaaagcg ccgctccgag gcgcccggca agcagggcca gcagaaacga
     4621 ccggcccacc aggagaaacg tccggagccc ggaacggccc gcagcgcggc aggaccgcgc
     4681 gaaccgggca aggtgaagtg gttcaaccga aacaagggct tcggcttcat catccgcgaa
     4741 tccggcgagg agatcttcgt tcaccagcga tcaatccgac ctgtgggcga gggggacaac
     4801 cgacgccggc cgagcctgcg cgacggtcag gcggtgacct tccaggttgc cgagcgcgac
     4861 aagggtattc aggcagaaga cgtcgtcgcc gaggactgat ccgctcatga acgtccgcgc
     4921 actgatcggc gaacggatcg aggcggcgct ggccgaaacc ggaaacccgg gggccccggc
     4981 tctggttcaa ccggccagcc gtcccgagtt cggcgactac caggccaacg gggtgatggg
     5041 agcggcaaag cgctccggga ccaagccgag ggaactcgcc gacgccgtcc tgaaggccgc
     5101 agcgctggac gacgttgcgg cgggcgtgga ggtggcgggc cccggcttcc tgaacatcac
     5161 gctggcagac gacttcctgt cggcacagct gctgccggaa gatcgcatgt cggccgtcga
     5221 gcacccgcaa accgtggtcg tggactactc c
//
LOCUS       DBNHQR010000038         9238 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_510254, whole genome shotgun sequence.
ACCESSION   DBNHQR010000038 DBNHQR010000000
VERSION     DBNHQR010000038.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9238)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9238)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9238
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_510254"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="AC16VR_00855"
     CDS             <1..456
                     /locus_tag="AC16VR_00855"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR6009727.1"
                     /translation="VLQDPGKTGGYIHGDCKINNLLFRENSAEVVCVVDLDTTMHGHW
                     AWDFGDLARSGAVTDAGALSLELFGALVEGFAGEKEAALGADELVLAPRYVCFMLGVR
                     FLTDHLDGDRYFKIAARGDNLRRARAQFRLLEEMEARESEMAEVAASRR"
     gene            complement(453..698)
                     /locus_tag="AC16VR_00860"
     CDS             complement(453..698)
                     /locus_tag="AC16VR_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2970 domain-containing protein"
                     /protein_id="HIR6009728.1"
                     /translation="MSIPKEDDHDPDPPERGPLTLTQTVASALAAAFGVQSSRNRTRD
                     FSRGRPAQFIAVGVVLTALLILGIAALVHLVLAGIES"
     gene            778..1173
                     /locus_tag="AC16VR_00865"
     CDS             778..1173
                     /locus_tag="AC16VR_00865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR6009729.1"
                     /translation="MLAGLRESPSGFAMTRCAALILVAILLCGCAVSSNRPTQLVSGS
                     AAYPEDARAQGIEGLVVVRYDVAVDGRVINARVVRAEPPDVFDDAALASVRSWRFNPA
                     LADGVPRATQDIESTVTFKLDGGDEYADY"
     gene            complement(1258..1896)
                     /locus_tag="AC16VR_00870"
     CDS             complement(1258..1896)
                     /locus_tag="AC16VR_00870"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR6009730.1"
                     /translation="MEFDIAATDDLLATTRAVRKRLDLTRPVPREIINECLELAVQAP
                     TGSNSQGWRWVVVDDAAKRKALADLYREAAGGYLSGAEEQANESGNQQTQRVFSSAIY
                     LMEHLHEVPVHVIPCLEGRPPEDAPPITYAGVYGSIYPAVWSFQLALRARGLGSTLTT
                     LHLFKEREAAELLNIPDNVMQVALLPVGYTVGTDFKRADRPPVAGITHWNEW"
     gene            1998..3287
                     /locus_tag="AC16VR_00875"
     CDS             1998..3287
                     /locus_tag="AC16VR_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016982861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR6009731.1"
                     /translation="MTLRRLAPLLLVLNAAGSLAEVSPEIRRGAEAVEAQVIAWRRDI
                     HAHPELSNREFRTAALVAEHLDSLGMEVTTGTAHTGVVGVLRGGLPGPVVALRADMDG
                     LPVEERTGLPYASKVRSTYNGLDVGVMHACGHDNHVAILMGAATVLSGMRERLPGTVK
                     FVFQPAEEGPPAGEDGGARMMVQEGVLEDPDVEAIFGLHISQADTVGFANYRPGGAMA
                     SAQRFEITITGRQTHGARPWQGVDPIVAGAHVVTALQTIVSRQVDISKAPAVVTVGSF
                     HGGVRNNIVPDSVKLEGTIRTFDADMRADIHSRIRRIATTVAESMGATADVYINDGVP
                     VTYNDPALTEAMLPTLHKVYGADRVRYALPITGAEDFSFFQEQVPGFYFFIGGRPADM
                     AAEDAIPNHSPLFFVDEGALKLGVQAMSQLAVDYLNR"
     gene            complement(3317..3964)
                     /locus_tag="AC16VR_00880"
     CDS             complement(3317..3964)
                     /locus_tag="AC16VR_00880"
                     /inference="COORDINATES: protein motif:HMM:NF017819.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HIR6009732.1"
                     /translation="MKGTGDSPAATAEYEPLLTCWFGPLEAGFADAAHRERWFAPTRS
                     FDAALAERFAPLVATAAAGGLDEWQQTPRGRLAYVLVCDQLPRNIYRGTARAFATDRR
                     AVAAAREAVRIGVDLALALEERAFLYLPFQHSEDILDQHLAVGLFTALRDASPKGYRD
                     LTGGYLRSAQQHRDLIERFGRFPHRNAALGRASTTEEAAYLEDGKTFGQAADAKG"
     gene            complement(3999..4925)
                     /locus_tag="AC16VR_00885"
     CDS             complement(3999..4925)
                     /locus_tag="AC16VR_00885"
                     /inference="COORDINATES: protein motif:HMM:NF024118.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6009733.1"
                     /translation="MRYLSLSITGLALLAAIGTAHAADAPFCGDEGVWVQILGAGGPE
                     LDDGQAGPSYLIWRDNHARLLVDAAPGSSAAFDRAGAEFEDLDAIAFTHLHADHSADF
                     PAFIKGSYFAERDRPLTVLGPSGEGVFPDTVTFVERMIGKDGAYPYLADFLTYGSAGG
                     YKVNARNVPSTGQRRWSRFGTEHIRLSAIPVHHDIVPAVAWRVEVGDKTIVFTGDFSN
                     QKNVIPSFAKDADALVVSHAIPETARGAARELHAIPSQLGQIAAQANARMLILGHRMN
                     RTRGRETQSREAIDKHYQGSVIFANDMECWGL"
     gene            complement(4980..6905)
                     /locus_tag="AC16VR_00890"
     CDS             complement(4980..6905)
                     /locus_tag="AC16VR_00890"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HIR6009734.1"
                     /translation="MDYTELGFIRVAAVAPELSIAEPATNAEKIVEHLTALEGRAVTI
                     ALFPELALTGYSCEDLFFSEQLHAAARSALLMLARSAGEAVCVVGTPYLLEDGRLLNC
                     AAVIADGRVRAMIPKIAQPNYGEFYERRWFVSGAGVDVSVDDPELGSFQVATNQLMRI
                     GNMRFAVEICEDLWAPEPPGIRHSLAGAELVVNLSASNELVAKADYRRDLVHMASARG
                     ICGYVYASSGPLESTKDLVFGGHLIACENGQLLGESPRYAFEPQTLITDIDIAKLRHD
                     RMQNTTFTNAPRPAPYHVVDVLQRPRPIAALERVYTAHPFVPDDEAQFEARAAEILDI
                     QTTGLARRCLAANTETLVIGLSGGLDSTLAFLVCLDTLDKIGRPRQSIQAITMPGPGT
                     TEHTLESARGLCAAAKVALKEIPIHEAVAQHLDALEHTGRDDLVFENTQARERTQILF
                     DYANKVSGIVVGTGDLSELALGWCTYNADHMASYNVNASVPKTMMAYLVRWYAKHRAG
                     EALAGVLQRVLQTPISPELIAPDTAGSISQETEAIIGPYELHDFFLFHFLRNGAGPAK
                     IFHLARLAFTGSYADDEIKRWLRLFFQRFFTQQFKRTTLPPGPKVGSVSLSPRGDWRM
                     PDEAGVQRLLADIDDLG"
     gene            6964..8085
                     /locus_tag="AC16VR_00895"
     CDS             6964..8085
                     /locus_tag="AC16VR_00895"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6009735.1"
                     /translation="MAILGMLMNFEFNEDQNLLREQARGFLADNCSPAVVRAVLEGDA
                     SYDADLWRKIVEMGYTATVIPEEFGGLGLSYLELSVIAEELGRVAAPVPFSSSVYLAT
                     EAILLAGSREQKETWLPRLAAGEVIGTFAFGESHGRPTAENLTARVTDGKLSGTKLPV
                     PDGGAADVAVVVASNGLYLVDLAQDGVAVTDVPTLDPTRGHSRIEFAGVQAEPLGDPA
                     DGWSLTEKVLDRAAVLFAWEQVGGSEASLEMAKEYALGRYAFGRPIASYQAIKHKLAN
                     MYVKNTLAKSNCYYGAWALNTDASELTLAAATARVSATQAYYFASKENIQTHGGMGYT
                     WEFDCQFYYRRAKLLSVNIGSEAHWQDRLITAVEQSNAA"
     gene            8095..>9238
                     /locus_tag="AC16VR_00900"
     CDS             8095..>9238
                     /locus_tag="AC16VR_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6009736.1"
                     /translation="MDFNDTAEEAKFREEVRGWLEVNVPTAEELAGLNYIEAAKLWQK
                     RKYDAGWACIRWPEEYGGRGASAIDQVIWNQEEAKYPDLPQGIFGIGQGMAAPTLMTW
                     ADDEAKARYLPRLASGEDIWCQLFSEPAGGSDLAALRTKAERDGDDWVINGQKIWTSG
                     AHYSDYGILVVRTDPTVPKHKGLSYFYLDMKSPGIEIKPIKQISGDSNFNEVYFTDVR
                     IPDSQRLGAVGQGWQVSLTTLMNERASIGGGGTAVGFDSVFKLAQRVNIGDRPAIEDS
                     QVRAKLADWFCQEAGLRFTGYRSMTALSRGEIPGPENSIGKLVGAPKTQDMASFAIDL
                     MEMTGAIWDKEFAEEAGLFQGTYMAIPGLRIAGGTDEIMANIIAERV"
BASE COUNT         1520 a         2935 c         3199 g         1584 t
ORIGIN      
        1 gtgctgcagg atccgggcaa gacgggtggt tacattcatg gcgactgcaa gatcaacaac
       61 ctgctctttc gtgagaacag cgcggaggtg gtctgcgtcg tcgatctgga caccaccatg
      121 cacggccact gggcctggga cttcggcgat ctggcgcgct caggcgcggt gacggacgcc
      181 ggggcgctgt ctctcgagct cttcggagcg ctggtcgagg ggtttgccgg tgagaaggaa
      241 gccgcgctcg gcgcggacga gcttgtcctt gctccgcgtt acgtctgctt catgctgggc
      301 gttcgcttcc tcacggatca tctggacggc gaccgctact tcaagatcgc cgcccgcggc
      361 gacaacctcc gccgcgcccg ggcgcagttt cgcctgctcg aggagatgga ggcgcgggag
      421 tcggagatgg ccgaggtcgc cgcgagtcgc cgttagcttt cgatgccggc gaggacgaga
      481 tgaacgagcg ccgcgatgcc gaggatcagc agggcagtga gcaccacgcc gacggcgatg
      541 aactgcgcgg gccggcctct gctgaagtcg cgcgtccggt tgcggcttga ctgcacgccg
      601 aaggcggcgg ccagagcgct ggccacggtc tgcgtcaggg tcagggggcc gcgctccggc
      661 gggtcgggat cgtggtcgtc ctctttgggg atgctcatgg ggggagtata gcgccgggcg
      721 agcgggcggc agcgtcgtcg accggcgcgg tcttgccagg gccggagcga aggtgccatg
      781 ctcgccggct tgcgggaatc accaagcggc tttgcgatga ctcgatgtgc ggccctcatt
      841 cttgtcgcga tcctcctctg cggatgcgcg gtatcctcga accggccgac ccagctcgtc
      901 tcgggcagcg cggcctaccc ggaagatgcc cgggcccagg gaatcgaggg gctcgtggtg
      961 gtccgctacg acgtggcggt ggacggccga gtgatcaatg cccgggtggt tcgcgccgag
     1021 ccgccggacg ttttcgatga tgcggcgttg gcttccgtgc gcagctggcg tttcaacccc
     1081 gctcttgccg atggcgtgcc ccgggcgacc caggacatcg agagcacggt gaccttcaag
     1141 ctcgacggcg gggacgagta cgccgattac tgatggcgtt cgggaccacc gcctgacccc
     1201 gaagatcctt tcagggtaac cgcgctcgtg gtgctcgggc tgggcgccgc ccggtcgcta
     1261 ccactcgttc cagtgggtga tgcccgccac cggcggccga tcggcgcgct tgaagtcggt
     1321 gccgacggtg taccccaccg gaagcagcgc aacctgcatc acgttgtccg gaatgttcag
     1381 cagctcggcg gcctcccgct ccttgaagag atgcagcgtc gtcagcgtgg agccgagccc
     1441 ccgcgcacgc agagccagct gaaagctcca cacggccggg tagatggacc cgtagacgcc
     1501 cgcgtaggta atgggcggag cgtcttccgg aggacgtcct tccaggcacg gtatgacgtg
     1561 gacgggcacc tcgtggaggt gttccatgag gtagatggcc gagctgaaca cccgctgggt
     1621 ctgctggttg ccggattcgt ttgcctgctc ctccgcgccc gacaggtagc cgcccgcggc
     1681 ctcccggtac aggtccgcca atgccttgcg cttcgccgcg tcgtcgacca cgacccagcg
     1741 ccagccctgg gagttcgacc ccgtcggcgc ctggacggcc agctccaggc actcgttgat
     1801 gatctcgcgc ggcacgggcc gcgtcaggtc gagccgcttg cgcacggccc gggtcgtggc
     1861 cagcaggtcg tctgtggcgg cgatatcgaa ttccatgctt gttccccctc tgaatgcccg
     1921 ggaagtttaa ctcactttgt gcgcatgctt gaggcgcatt aagctcgccc aaaccgggga
     1981 cggctgagga atcccagatg acgctgcgca gactcgctcc tctacttctc gtgctcaacg
     2041 ctgccggctc cctcgccgag gtctcgccgg agattcgccg gggagcggaa gccgtggagg
     2101 cccaggtcat tgcctggcgc cgggacatac atgcccatcc agagctttcc aaccgcgagt
     2161 tccgcacggc ggccctcgtt gccgagcacc tggacagtct cggcatggag gtgaccaccg
     2221 gcactgccca tacgggggtg gtgggcgtgc tgcgaggcgg actgccaggt cccgtggtgg
     2281 ccctgcgcgc cgacatggac ggtcttcccg tagaggagcg cacgggattg ccctatgcct
     2341 ccaaggtccg ctcgacctac aacggcctgg acgtcggggt gatgcacgct tgtgggcacg
     2401 acaatcacgt ggccatcctc atgggcgcgg ctacggttct cagcggcatg cgcgagcggc
     2461 tgcccggcac ggtcaagttc gtttttcagc cggccgagga gggcccgcct gccggtgagg
     2521 acggtggggc gaggatgatg gtgcaagagg gcgttctcga ggatcccgac gtggaggcca
     2581 tcttcggtct gcacatcagc caggcggata ccgtgggttt tgccaactat cggcccggcg
     2641 gcgccatggc cagcgcccag cgcttcgaga tcaccatcac cggccgccag acccacgggg
     2701 cccggccctg gcagggcgtc gaccccatcg tcgccggtgc tcacgtcgtc acggcgctgc
     2761 agaccatcgt cagccgtcag gtggacatca gcaaggcgcc cgcggtggtc accgtcggct
     2821 cgttccacgg tggcgtgcgc aacaacatcg tcccggactc cgtcaagctc gagggcacca
     2881 tccgtacctt cgatgccgac atgcgggcgg acattcattc aaggatccgc cgcatcgcga
     2941 ccaccgtggc ggaatccatg ggcgcgacgg cggacgtcta catcaacgac ggcgtcccgg
     3001 tgacctacaa cgatcctgcg ctaaccgagg ccatgctgcc caccctgcac aaggtctacg
     3061 gtgccgatcg ggttcgctac gcattgccca tcaccggggc cgaggacttc tcgttcttcc
     3121 aggagcaggt gcccgggttc tatttcttca tcggagggcg ccccgcggac atggcggcgg
     3181 aggacgcgat tcccaatcac tcgccgctgt tcttcgtgga cgaaggcgcg ctgaagctcg
     3241 gcgtccaggc catgagccag ctcgcggtgg attatctgaa tcgctgattg actgcaaagg
     3301 acaggctgca agagcgtcag cccttggcgt cagcggcttg tccgaacgtc ttgccgtcct
     3361 cgaggtaggc cgcctcttcg gtggtggaag cgcgtccgag ggcggcattg cggtgcggaa
     3421 agcggccgaa tctctcgatg aggtcgcggt gctgctgagc ggaccgcaga tatccgcccg
     3481 tcagatcgcg gtagcccttg gggcttgcgt ccctcagggc ggtgaaaagg cccacggcca
     3541 ggtgctggtc caggatgtcc tcggagtgct ggaagggcag gtacaggaag gcccgctcct
     3601 ccagggcgag ggcgagatcc acgccgatgc gaactgcctc ccgggcggcg gccacggccc
     3661 ggcggtcggt ggcgaaggcc cgggccgtgc ccctgtagat gttgcgcggc agctgatcgc
     3721 agaccagcac gtaggccagg cggcccctcg gcgtctgctg ccactcatcg agcccgcccg
     3781 cggcggcggt ggccaccagc ggcgcgaagc gttcggcgag ggcggcgtcg aacgagcggg
     3841 tcggggcaaa ccagcgctcc cgatgggccg cgtccgcgaa gcccgcctcg agcggtccga
     3901 accagcaggt aagaagcggt tcgtactcgg ctgtggccgc cggcgagtcg cctgtccctt
     3961 tcacccgagc gcttcgcccc gcccctgacg cgagtggctc aaagccccca gcactccatg
     4021 tcgttggcga atatcaccga gccctggtag tgcttgtcga tggcctcgcg gctctgggtc
     4081 tcacgaccgc gggtccggtt catgcggtgc ccgagaatca gcatgcgtgc gttggcctgg
     4141 gcggcgatct ggccgagctg gctggggatg gcgtgcagct cccgcgcggc gcctcgcgcc
     4201 gtctccggga tcgcgtggct gaccaccagc gcgtctgcgt ccttggcaaa tgaggggatg
     4261 acgttcttct ggttgctgaa atccccggtg aagacgatgg tcttgtcgcc cacctccacc
     4321 cgccaggcca cggcgggaac gatgtcgtga tgaacgggga tggcagacag cctgatgtgt
     4381 tcggtgccga accgcgacca gcgacgctgc ccggtggaag gcacgtttcg cgcgttcacc
     4441 ttgtagcctc ccgcggagcc gtaggtcaga aaatcggcga ggtaggggta cgcgccatcc
     4501 ttgccgatca tccgttccac gaacgtcacg gtgtcgggga agacgccctc gccggacggg
     4561 ccgagcaccg tcagtggccg gtcgcgctcg gcaaagtagg atcctttgat gaaggcgggg
     4621 aaatccgccg agtggtcggc gtgcaggtgc gtgaacgcga tggcgtccag atcctcgaat
     4681 tcggccccgg cgcggtcgaa ggcggcggaa gaaccgggcg ccgcatccac cagcaggcgg
     4741 gcgtggttgt cgcgccatat caggtagctg ggtccggcct ggccgtcgtc gagctccggc
     4801 ccgcctgcgc cgaggatctg cacccagact ccctcgtcgc cgcagaacgg ggcatcggcg
     4861 gcatgagcgg tgccgatagc ggccagcagt gccaggccgg tgatgctcaa gctgaggtaa
     4921 cgcatgctga agtcctcggt tcggttatcg ggtcgtttcg ttcaaggggc ccctggcgtt
     4981 cagcccaggt cgtcgatgtc ggcaagcagg cgctggacgc cggcctcgtc gggcatccgc
     5041 cagtcgccgc ggggcgacag gctcacggag cccacctttg gacccggggg aagggtggtg
     5101 cgcttgaact gctgggtgaa gaagcgctgg aagaatagcc tcagccagcg tttgatctcg
     5161 tcgtccgcgt aactcccggt aaatgccagc ctggccagat gaaagatctt tgccggaccg
     5221 gccccgtttc tcaggaagtg gaagaggaag aaatcatgca gctcgtaggg gccgatgatg
     5281 gcctcggttt cctggctgat gctgcccgcg gtgtcgggcg cgatcagctc cggcgagatg
     5341 ggcgtctgca ggacgcgctg caggacgccg gccagcgctt cccccgcgcg gtgtttggcg
     5401 taccaccgca ctaggtaggc catcatggtt ttcggcacgc tggcgttgac gttgtagctc
     5461 gccatgtggt ccgcgttgta cgtgcaccag ccgagcgcca gctctgacag gtcgccggtt
     5521 cctacgacga tgcccgaaac cttgttggcg tagtcgaaga gaatctgggt gcgttcccga
     5581 gcctgggtgt tttcgaacac cagatcgtca cgtccggtgt gctccagcgc gtcgaggtgc
     5641 tgcgccaccg cctcgtgaat ggggatctcc ttgagcgcca ccttcgcggc ggcgcagagc
     5701 cccctcgccg actccagagt gtgctcggtg gtcccagggc ccggcatggt gatggcctgg
     5761 atggactggc ggggacggcc gatcttgtcc agggtgtcca ggcagacgag gaaagccagg
     5821 gtggagtcga gcccgcccga caggccgatg acgagagttt cggtattcgc agcgaggcat
     5881 cggcgggcga gccccgtcgt ctggatgtcg aggatctcgg ccgcccgggc ctcgaactgg
     5941 gcctcgtcgt cgggaacgaa cgggtgagcc gtatagacgc gctccagagc cgcgatgggc
     6001 cgggggcgct gcaggacgtc cacaacgtgg tagggcgccg ggcgcggcgc gttggtgaac
     6061 gtcgtgttct gcatccggtc gtgacgcagc ttggcgatgt cgatgtccgt gatcagcgtt
     6121 tggggctcga acgcgtaacg cgggctctcg cccaggagct ggccgttctc gcaggcgatg
     6181 aggtgcccgc cgaacaccag gtccttggtc gactccagcg ggccgctgga cgcgtagacg
     6241 tagccgcaga ttcccctggc gctggccatg tgcaccagat cccttcgata gtcggctttg
     6301 gccacgagct cgttgctggc ggacaggttc acgaccagct cggctccggc caggctgtga
     6361 cggatgccgg gcggctccgg cgcccagagg tcctcacaaa tctccaccgc gaaccgcatg
     6421 tttccgatgc gcatcagctg gttggtagcc acctggaagg acccgagctc agggtcgtcg
     6481 acgctgacgt ccacccctgc gccggagacg aaccagcggc gctcgtagaa ctcgccgtaa
     6541 ttcggctggg cgatcttggg gatcatggcg cggaccctgc cgtcggcgat gacggccgcg
     6601 cagttcagca gcctgccgtc ctccagcagg tacggcgtgc cgacgacgca gacggcctcg
     6661 ccggcggatc gggccagcat gaggagggcc gaacgggcgg cggcatggag ctgttcgctg
     6721 aaaaagaggt cctcgcacga gtagccggtc agcgcaagct cggggaacag cgcaatggtt
     6781 acagcgcgtc cctcgagcgc tgtcaggtgc tcgacgattt tctccgcgtt ggtggccggt
     6841 tcggcgatgg agagttccgg cgccacggcg gcaacgcgga tgaagcccag ttcggtgtag
     6901 tccatggtgc acagtttaac tgggctcgcg ttttaaaccc aaagcatgga aaatcgcccg
     6961 ttcttggcga ttctggggat gctcatgaac ttcgaattca acgaagacca gaacctcttg
     7021 cgggagcagg cgcggggctt tctcgccgac aattgctctc cggcggtggt cagagccgtg
     7081 ctggagggtg atgcctccta cgatgccgac ctctggcgga agatcgtgga gatgggttac
     7141 acggccacgg tgattcccga ggagttcggg gggctcgggc tctcctacct ggagctgtcc
     7201 gtgatcgccg aggagctcgg tcgcgttgcc gctccggtgc cgttctcgtc atccgtttac
     7261 ctggcaaccg aagccatcct gctggctggc tcccgggagc agaaggaaac ctggctgccg
     7321 cgactggccg ccggcgaggt gatcggaacg ttcgccttcg gcgaaagcca tgggcgcccc
     7381 accgcggaga acctgaccgc ccgcgtgaca gacggcaagc tctccggaac caagctgccg
     7441 gtgcccgacg gcggggcggc agacgttgcc gtggtcgtgg cgagcaacgg gctgtacctg
     7501 gtggatcttg cccaggacgg ggttgcggtg acggatgttc cgaccctgga cccgacccgc
     7561 ggccacagcc gcatcgagtt cgcgggcgtt caagcggagc ccctgggcga ccccgccgac
     7621 ggttggtcgc tcaccgagaa ggtgctcgat cgggctgccg tgctgttcgc ctgggagcag
     7681 gtgggcggct cggaagcgtc gctggagatg gccaaggaat acgctctcgg gcgctacgct
     7741 ttcggccgcc ccatcgcctc ttatcaggcc atcaagcaca agctggccaa catgtacgtg
     7801 aagaacaccc tggccaagtc taactgctac tacggggcgt gggcgctcaa cacggacgca
     7861 tcggagctga ccctggccgc ggcgacggcg cgggtgtccg ccacccaggc ctactacttc
     7921 gcctccaagg aaaacatcca gacccatggc ggcatgggat acacctggga attcgactgt
     7981 cagttctact atcgccgggc gaagctgctg tccgtgaaca tcggcagcga ggcccactgg
     8041 caggaccgtt tgatcaccgc cgtcgagcag agcaacgcgg cataaggagc agtcatggat
     8101 ttcaacgaca ccgcggaaga agcaaaattc agagaggaag tgcgcggctg gctcgaagtc
     8161 aacgtgccca ccgccgagga gctcgcaggg ctcaattaca tcgaggcggc caagctgtgg
     8221 cagaagcgca agtacgacgc cggctgggcc tgcattcgct ggccggagga gtacggcggg
     8281 cgcggcgctt cagccatcga tcaggtcatc tggaaccagg aggaagccaa gtatcccgat
     8341 ctgccgcaag gcatattcgg catcggccag ggcatggccg cccccacgct gatgacctgg
     8401 gcggacgacg aggccaaggc ccgctatctt ccgcgccttg ccagcggcga ggacatctgg
     8461 tgccagctgt tcagcgagcc cgccgggggc tcggaccttg cggcgctcag gaccaaggcg
     8521 gagcgtgacg gcgacgactg ggtgatcaac ggccagaaga tctggacctc gggcgcgcac
     8581 tactcagact acgggatcct cgtggtcagg accgacccca cggtgccgaa gcacaagggg
     8641 ctgagctatt tctacctgga catgaaatcc ccgggcatcg agatcaagcc gatcaagcag
     8701 atttccggcg actcgaactt caacgaggtc tacttcacgg acgtgaggat tcccgacagc
     8761 cagcgcctgg gcgccgtggg tcagggctgg caggtttcgt tgaccacgct gatgaacgaa
     8821 cgggcttcca tcggcggtgg cgggaccgcc gtcggcttcg actccgtttt caagctcgcc
     8881 cagcgggtca acatcggcga caggccggcc atcgaggact cccaggtgcg ggcgaagctt
     8941 gccgactggt tctgtcagga agccggtctg cgcttcaccg gctaccgctc catgacggcg
     9001 ctttcccggg gcgagatccc gggaccggaa aactccatcg gcaagctggt cggcgcaccc
     9061 aagacacagg acatggcgtc tttcgccatc gacctgatgg aaatgacggg cgcgatctgg
     9121 gacaaggagt tcgccgagga ggccgggttg ttccagggaa cctacatggc cattccgggc
     9181 ttgaggatcg ccggcggcac cgacgagatc atggcgaaca tcatcgccga gcgggtgc
//
LOCUS       DBNHQR010000039         2828 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1587351, whole genome shotgun sequence.
ACCESSION   DBNHQR010000039 DBNHQR010000000
VERSION     DBNHQR010000039.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1587351"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /locus_tag="AC16VR_00905"
     CDS             complement(<1..1112)
                     /locus_tag="AC16VR_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIR6009737.1"
                     /translation="MSEQDHESEKAAEAQPESATAEKAVKRGGQMIGLVIVVTLVWYL
                     LADRYTPYTTQARVQGYVIGVAPKVSGLVTQVWAQNNREVQQGERLFEIDRSEYQIAV
                     GKARSDLENARRQVEAGRAAVEASRANLAAALANRVRAEKDYDRLQRLHSRDPGTIST
                     RRLEMSRASLDQAVAAVSAAESNVQAAIEQMGGEDEASNAILQSAQSAVARAELDLSN
                     TIVTASSRGVITDLRADVGQFAGAGSAVMTLIAVHDLWISAEFTENNLGHMRSGTEVE
                     ILFDVVPGRVFSGRIRSLGLGVSAGQSHPPGTLPTIQNNRDWLRQSQRFAVIVDFDPN
                     QHGELRSQLRIGGQASVMGLTEGHGVLNLLGQLYMR"
     gene            complement(1109..2647)
                     /locus_tag="AC16VR_00910"
     CDS             complement(1109..2647)
                     /locus_tag="AC16VR_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007957057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR6009738.1"
                     /translation="MLVLLGVLAAQGACTVLGPDYEEPEVGWLGDWQTDLYGRLEEPE
                     AGTQSDLSFWWMLFGDPTLNALVETAKRDNIGLRVAGLRILESRAVLGIAGSARYPQV
                     QQVTGAVTYVTSREHGGASNNGSNDLASYDAGFGLGWELDFWGRFRRGIESADAAFFA
                     SLTNHQDAQVLLSAQVADLYYALLTTRMRIGIARANAEIQRRSLEITQELFDSGQDSE
                     LDVQQARTQYLTTVSTIPELEIVEVKVLNALSALLGRPPGEVPELADMPDRLPGLDPS
                     SIEAIPARLLTRRPDVRAAAWGVAAQSAQIGIAQADYYPAIAVVGTVGWAGNSLSATP
                     DVGTLAAGPALTWNVFDHGRIRNNVRVQDARLQQAIAGFQNTVLQAAREIDDAAITLV
                     KTRERQATLADAVLSARRALDLARVRYREGYSDFQRVLDAQRALFGAAERDLVNKGNH
                     ISAVIQFYKALGGGWTEMPIGELLPETVREKMRTRSNWGDLLDAPLPVNQEGPVPAEG
                     GTTP"
BASE COUNT          485 a          961 c          876 g          506 t
ORIGIN      
        1 atgcgcatgt agagctgacc cagcaggttc agcaccccgt gcccttcagt gagacccatc
       61 accgacgcct ggcccccgat gcgcagctgc gagcgaagtt cgccgtgctg gttcggatcg
      121 aagtccacga tgacggcaaa gcgctgggac tgtctcagcc agtcccggtt gttctgaatg
      181 gtaggcagcg tccccggcgg atgagactgg ccggcgctca ctccgaggcc gaggctgcgt
      241 atcctgccgg aaaagactct gcccggcacc acgtcgaaga ggatctccac ctcggtgccg
      301 cttcgcatat gccccaggtt gttctccgtg aactcggcgc tgatccaaag gtcgtgcacc
      361 gcgatgagcg tcatgaccgc gctgcccgca ccggcgaact ggccgacgtc cgcacgaaga
      421 tccgtgatca cgccgcggga agaagccgtg acgatggtat tggaaagatc cagctccgcg
      481 cgcgcgaccg cgctctgtgc ggattgcaga atggcgttgg aggcttcgtc ctcgcccccc
      541 atctgctcga tggcagcttg gacattcgac tcggccgccg agaccgcagc taccgcctgg
      601 tcgagcgagg ctctggacat ttccagccgc cgggtggaga tcgtcccggg atcccgggag
      661 tgaagtcgct gaagacgatc gtagtccttc tccgccctga cccgattggc gagggcagcg
      721 gcgaggttgg cgcgagaagc ctctaccgcg gcgcgtccgg cctctacctg tctgcgggcg
      781 ttttccaggt ccgagcgcgc cttgcccacg gcaatctggt actcagagcg gtcgatctcg
      841 aacagtcgtt ccccttgctg gacctcgcga ttgttctgcg cccacacctg ggtcacgagg
      901 ccggacacct tcggtgccac cccgatgacg tagccctgca cccttgcctg ggtggtatat
      961 ggcgtgtagc ggtctgccag gaggtaccag acgagggtga cgacgatcac cagaccgatc
     1021 atttgaccgc cacgcttgac ggctttttcg gccgttgccg attccggttg agcttccgcg
     1081 gccttctccg actcgtgatc ttgctcgctc atggggttgt ccctccttcg gccggcacag
     1141 ggccctcctg attcacgggc agcggcgcat cgagcaggtc accccaatta ctccgagtgc
     1201 gcatcttctc ccgaacggtc tcgggcagca gctcaccgat gggcatctcc gtccaaccac
     1261 ccccaagggc cttgtagaac tgaatgacgg cgctgatgtg gtttcccttg ttgaccagat
     1321 cccgctcagc ggccccgaac agcgcccgct gggcatccag cacgcgctgg aagtcgctgt
     1381 agccctcgcg gtagcgcacc cgggccagat cgagagcccg ccgcgctgac agcacggcgt
     1441 ccgccagcgt cgcctgccgc tccctggtct tcacgagcgt gatggccgca tcgtctatct
     1501 cgcgggccgc ctgaagcacg gtgttctgga agccggcgat tgcctgctgc agccgggcat
     1561 cctgaacacg aacgttgttg cggatgcgac catggtcaaa gacgttccag gtcagcgcgg
     1621 ggccggcagc gagggtcccc acatccggcg tggcgctgag gctgtttcca gcccaaccca
     1681 cggttcccac caccgcaatg gcgggatagt aatcggcctg ggcgattccg atctgcgcag
     1741 actgtgccgc caccccccag gccgccgcgc gaacgtccgg ccggcgcgtt aagagccgcg
     1801 ccgggattgc ctctatcgac gagggatcca agccaggcaa cctatccggc atgtcggcga
     1861 gttcgggcac ctcgcccggc gggcgcccga gaagcgccga caaggcgttc aggactttga
     1921 cctcgacgat ctccagttcc gggatcgtgg acacagtggt caggtactgg gttctcgcct
     1981 gctgcacgtc gagctcggaa tcctgaccac tatcgaagag ctcctgggtg atctccaggc
     2041 tgcgacgctg aatctccgca ttggcgcgcg cgatcccgat tcgcatccgt gtggtcagca
     2101 gcgcgtagta caggtcggca acctgagcgc tgagcagaac ctgagcgtcc tggtggttgg
     2161 tcagggaggc gaagaaggcg gcgtcggccg actcgatccc gcgcctgaag cgtccccaga
     2221 aatcaagctc ccagcccaga ccgaagcccg catcgtagct ggcgagatcg ttggatccgt
     2281 tgttcgatgc gccgccgtgc tcacggctgg tcacgtaagt taccgcaccg gttacctgct
     2341 ggacctgagg gtagcgggcg ctgcccgcga tcccgagcac ggcccgactc tccagaatac
     2401 gcagacccgc gacacgcaat ccgatgttgt cgcgtttcgc agtctccacc agcgcgttta
     2461 gcgtggggtc gccaaacagc atccaccaga aagaaagatc cgattgagtg ccggcttcag
     2521 gctcttcgag ccggccatag agatccgtct gccaatcacc cagccacccc acctcgggtt
     2581 cctcgtaatc gggcccgagg acggtgcagg ctccttgggc ggccagtacc cccagcagca
     2641 ccagcaccga cgccgtcggc gacttgctgg gcgagaacac gttatcaggc tcggggttcg
     2701 gggacggtcc cgccctctag ctctgctgca gagccttccg gagacgtttc ggctccggcg
     2761 ccgccattga cccaatccat gaagagctgg tagcccaccg ccaggatcac ggcgccgacg
     2821 aacagccc
//
LOCUS       DBNHQR010000040        12442 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1650963, whole genome shotgun sequence.
ACCESSION   DBNHQR010000040 DBNHQR010000000
VERSION     DBNHQR010000040.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 12442)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12442)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12442
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1650963"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="AC16VR_00915"
     CDS             <1..434
                     /locus_tag="AC16VR_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009739.1"
                     /translation="TFTAIRLGLVPMVAPADQHLPFIEVGDLADAVNAWSQHPQACSG
                     GTFALHDGQQGGYDWQAIRQQIGPTRTVTLKVPYPLLDALSHVNLWLSRIMSYSPMLT
                     PGKVRELTYMGWTCSNEAFTDATGWVPRTRLGQGVAAMFAR"
     gene            457..708
                     /locus_tag="AC16VR_00920"
     CDS             457..708
                     /locus_tag="AC16VR_00920"
                     /inference="COORDINATES: protein motif:HMM:NF012759.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR6009740.1"
                     /translation="MSASQTDIIVTLAELLCAQLPEPVAVAPDTDLISDLALESVQIM
                     EFIVEVEDHYDIAISLEALAEIRNLDQLARLVITALDAQ"
     gene            705..1892
                     /gene="spt"
                     /locus_tag="AC16VR_00925"
     CDS             705..1892
                     /gene="spt"
                     /locus_tag="AC16VR_00925"
                     /EC_number="2.3.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020797731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004758 - serine C-palmitoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046513 - ceramide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine palmitoyltransferase"
                     /protein_id="HIR6009741.1"
                     /translation="MSLLEKFDALETTRQELLGHGTNPTDVIIERVISATESVIGGKP
                     TIMAGTNNYLGLTFDPECIAAGKRALDESGTGTTGSRMANGNYASHGELEREIADFYG
                     YPYAMVFSTGYAANIGTMAALVGPGDAVMLDGDAHASLFDGSKLSGATVYRFKHNDAD
                     SLDKRLRRLGDVASRTLVIVEGLYSILGDRAPLKELAEVTRAHDACLFVDEAHSLGVL
                     GETGRGRVEEDQVEADFVVGTFSKSLGSTGGFCVSPHRALTLFRYASRPYMFTASPCP
                     SVIATTRAALAQMRKRPELRASLWRNAETLYSGLAQLGFTLGPEPSPVVAVRMTSREQ
                     ALANWQQLLDNGIYILLILPPAAPDGTALLRCSVSAAHTPEQIQRIIDAFRQLPPDPG
                     QPA"
     gene            complement(1855..3168)
                     /locus_tag="AC16VR_00930"
     CDS             complement(1855..3168)
                     /locus_tag="AC16VR_00930"
                     /inference="COORDINATES: protein motif:HMM:NF024832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HIR6009742.1"
                     /translation="MNESVSDSPVRRALKNFSVLMGGRAVAGVLSVVSLTLVARFLGP
                     VEFGLLVVVHTTAVVVRGLLNFKPSDTVVRYGVAPFDDDDHRELNHLLRFTLALDVAT
                     ASLAALCMMLVMLLAGPALGLPEELAMPAVLYGLVLLASGSGTARGVLRVAGRFHAMG
                     VQQNVGPLIRLAGVAAVYLAEGGLLGYLMVWAVAFAAEHLFMNVRGWLELKRRGVRPG
                     RPTLAGAKRRFPGLWGFVGTLYWQSNLDLAQRHGLILLAGVLLGPAGAGMFRVAREFA
                     DVLAKPVIVVRQAVFPDFARLWRERDPRFKTLYWRLGLYGGLIAAVVVGLVGLYAGEL
                     LEALVGEEYVHGAALLVWLMVAGALELIGAALRPAAYAMGAATGALRVQIVTAVCHVS
                     AFAVLVPWLGLAGAGIAAACSGAIALAGMVALVLTRADQDPGAAA"
     gene            complement(3218..4135)
                     /locus_tag="AC16VR_00935"
     CDS             complement(3218..4135)
                     /locus_tag="AC16VR_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009743.1"
                     /translation="MRDARSVIVERTAPAGLENDQGVERMLSELRARCGEHLQAVLLY
                     GSYLRGVRDTLLDFYVVVDGYRGALGSPVSALGARVLPPNVYYLAVGEGAERVRAKYA
                     VVTMRQLRRQAASGLHPYFWARFAQPARIVYARWPADEAALVQIIEQCVRTFVKRVAP
                     TLRGDFTSREFWARGFALTYRSELRSESAGRSDSIIDHDPDYYDNMLAAVADGRHLVC
                     AGGRYRAVRPRVSAAVEWACVIALGKFLSVGRLVKGALTFDDALDYILWKVERHSGVR
                     VEASARQHRYPLIFAWPLVWRLYRRGAFR"
     gene            complement(4148..5332)
                     /locus_tag="AC16VR_00940"
     CDS             complement(4148..5332)
                     /locus_tag="AC16VR_00940"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR6009744.1"
                     /translation="MSIYAHLLGDSDIELWGLTSRERLRRQVVELPNAVLVDQLGTVP
                     EDATVICIRADYLFESRTIKALAERGRAVLVCPNDGGAAALVSTGREAAQLVPLLEGA
                     TTDHDLPTLAPADLAAYEDRLRKAEEPLLLPIAEEDRGELEDRLYGRSYKGITDLVTK
                     WLWPRPARHAVRVCARLGITPNMVTLAGLLLVVYAGFAFYQGDYWLGLAAGWFMTFLD
                     TVDGKLARVTVSSSRIGHVLDHGMDIVHPPFWYVLWGMSLDDSGFSVPLLEYYWWIVA
                     GYVGGRLIEAAFHGLGSCSLFDWRPFDAYFRLVTARRNPCLILMLVAMLLGSPGGAFL
                     AVVFWTVGTTVVLMIRLLQATLARMSDGPLSSWLTEPSAATAYPRAFRTFSRTRGAYV
                     QR"
     gene            5454..6365
                     /locus_tag="AC16VR_00945"
     CDS             5454..6365
                     /locus_tag="AC16VR_00945"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR6009745.1"
                     /translation="MRLIHLSDPHLSSLHGVKLRDLLGKRTLGYQSWYRKRRHQYTMD
                     TLRRVTRAVRNEAADAIVVTGDLVHLGMAQELRQAREWLESVGGPDRVLVIPGNHDCY
                     HRQSWALVKETIGPYLALEKDTADARDGYPLVRRSGEASVIAAGTAEPTAWWSASGSL
                     GAAQTTRLEDALIKARETFTFLAIHHPPLPGMAPRRKSLRDAHALAELLERHAPDVVL
                     HGHLHANQENSDTPTRIFCTAPPSSERPRAPASYRVFDVVEDASGYRVVMRLKSLIGE
                     EMVVADEQSWTSGRSLDPGERDDAAKQ"
     gene            complement(6324..7391)
                     /gene="lptG"
                     /locus_tag="AC16VR_00950"
     CDS             complement(6324..7391)
                     /gene="lptG"
                     /locus_tag="AC16VR_00950"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HIR6009746.1"
                     /translation="MKRVDRYLVGVLLLGAVPVLLLLLGLFGFVTLAEELEDVGKGQY
                     EEADAIRVALLTLPRIAIDLLPVAVLLGGVMALGNLAQEQELTALRAVGLSQWRMAYP
                     LALAAIVLSYVVLLAQQFVVPRFEEQAKELQAKSLVNTMRGGETTEFWTRDGTHFIRV
                     GDVRFGMIPADVEIYDLGDRGVVQRVLQAEYADVIGPGQWLLHGVEERRILGKEVRRT
                     GRDTYEWESFLSPDQMASLIAPMETLTLGDLYRYVEYLEASGLNAHRYRFRLWQVLSS
                     PIALMAMCLLGLPFVLGSVRARPAGARVLFGATVGIGFYLVEQTSAQLALLYELPVIP
                     LAMLPDSALLLGSIVALTRVE"
     gene            complement(7388..8458)
                     /gene="lptF"
                     /locus_tag="AC16VR_00955"
     CDS             complement(7388..8458)
                     /gene="lptF"
                     /locus_tag="AC16VR_00955"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HIR6009747.1"
                     /translation="MIINRYLVRELLLPFFAVGGVLTVIFTTYSMTRFLDKAADGLLR
                     TSEVLQLTALKSFIAQEVLLPVSLFVALVLALGRLYSDWEMTALRSFGFAERQVLFPI
                     MSVALGFALVVAVFSLVGRPWAYEHLYRLEVEAEATSELERIKPGRFYLYDDNARTVF
                     IEELKAEDDEIQGLFVRALDGDDLEIISAPSGTVSEYVSDKNHQVDLRDAQVYRKTAQ
                     GADFLGIFGELRIFVPVAAVQPVGYKPKSQNSFVLRESDALVDQAEYQWRLSAPVSTI
                     LLALLAVLLGRSRPRQGRFARLTVAVVIYAVYFNVLGIGRTWVEQGTAATLWWVPATF
                     AGGIVLAYVPWARWFRRRGRSA"
     gene            8548..9057
                     /locus_tag="AC16VR_00960"
     CDS             8548..9057
                     /locus_tag="AC16VR_00960"
                     /inference="COORDINATES: protein motif:HMM:NF015901.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HIR6009748.1"
                     /translation="MLKRFFAAELASAIALALVQLSCSAWAGELPALDDQDTVVVTAD
                     QAWESEDGDSLSFAGNFNLNAPDFHVASDTAVIHGPLEDPNRIVAEGSPVRFWVADPD
                     SGNRTHGTGRRLEYDRQRDLVRLEGDAVLEDDRTVMRSSELEYNTATKRLVAQGSGGV
                     EIVTNPNRD"
     gene            complement(9067..9495)
                     /locus_tag="AC16VR_00965"
     CDS             complement(9067..9495)
                     /locus_tag="AC16VR_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIR6009749.1"
                     /translation="MNATAEKFGYPDTLVREYGHWLVLVRPQQVSLGSLVLVCKEPVT
                     RFSEVSRAAFTELHDVIAAAEGALSSLYSYDRINYLMLMMVDPDVHYHVIPRDEAARL
                     LGTVEIADHGWPGPPALGEFHELDAAEREALLGQLREAWS"
     gene            complement(9497..10249)
                     /locus_tag="AC16VR_00970"
     CDS             complement(9497..10249)
                     /locus_tag="AC16VR_00970"
                     /inference="COORDINATES: protein motif:HMM:NF024214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HIR6009750.1"
                     /translation="MKAIILSAGQGRRLLPLTAGTPKCGVEIAGQSLLEWQLGEVAQC
                     GIDEVVVVTGFHADIVDDLVSRCSMVPVRTLHNPFFDASDNLGTCWVARSEMTDPFVI
                     INGDTLFEAAILQKLLASPANTPVTLVVDRKDHYDEDDMKVVVSDGRLRRVGKRLELD
                     DVNGESIGMMAFRGLGPAKFVSKVESLMRSDMGLKLWYLSAVDQLAQSGEVGVCDIHG
                     LSWCEVDDRDDLDNAAGVVTAWRKPASRAAAD"
     gene            complement(10302..11912)
                     /locus_tag="AC16VR_00975"
     CDS             complement(10302..11912)
                     /locus_tag="AC16VR_00975"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6009751.1"
                     /translation="MLLDLEKLPQGERFQADVCIAGAGAAGVALARRLAELGHSICLL
                     ESGGLDFEQSTQDLYAGTSDGMTYYDLVDARLRFLGGTTNIWGGRCALLDDIDFRRRP
                     WVPHSGWPITNAELTPYYAAVHDELELGEVRYDESLWDEVGERVPPFDPDAVSTRFWR
                     FDDVRERFAARRVQDLASSAAVTLVLHANVTHIQAAANAASIEHLKVSSLSGIEGRVT
                     ARHYVLACGGIENARLLLASRDVEAAGVGNGNDQVGRYFMEHPHGRAGRVSGPGAFPL
                     WAAFRKRFRSHGPDLAPVLVPSAAAQEREGILNSAFTLSLQRDPERGVAVTRQMYQTL
                     KHGMAPTRRGRTLWHAYRAVRSSAQRLARVPFERLRAGLGMRRLYLMIRAEQAPNPDS
                     RITLGSERDALGMPRAALSWQLGELDKRTIQVMVRLLDGELTRLGLGSVEASSWLGEP
                     GNDWPVDPTVGNHPIGGYHHIGTTRMSADPRHGVVDGSGRVHGYGNLFVAGSSVFATA
                     GWANPTLTILALALRLGDHLHGLLGGKS"
     gene            complement(11915..>12442)
                     /locus_tag="AC16VR_00980"
     CDS             complement(11915..>12442)
                     /locus_tag="AC16VR_00980"
                     /inference="COORDINATES: protein motif:HMM:NF016974.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HIR6009752.1"
                     /translation="PAFHQLSPDQKRISKGDNWKTFAFYVFGIRLEENCRRCPVTAAT
                     IECLPGLQNAWFSILAPRYHIPPHKGPTRAVIRCHLGLAVPRDGERCWLRVDDQIRHW
                     EAGGCLLFDDTFEHEVRNDTDETRIVLFLDVDRPSDRLGTWFNRLIVTLIRGSAYVKD
                     PLKNLADWNRARREA"
BASE COUNT         2373 a         4264 c         3924 g         1881 t
ORIGIN      
        1 gcacgttcac cgcaatccgg ctcggcctgg tgcccatggt cgctccggca gaccagcatc
       61 tgcctttcat cgaggtcggc gatctggccg atgccgtgaa cgcctggagc caacatccgc
      121 aagcctgctc aggcgggacc tttgccctgc acgacggcca gcagggcggc tacgactggc
      181 aggcgatccg ccagcagatc ggtccgaccc gcacggttac gctgaaagtg ccgtacccgt
      241 tgctcgacgc gctgagccac gtaaacttgt ggctttcgcg catcatgagc tactcgccca
      301 tgctcacgcc gggcaaagtg cgcgaactga catacatggg ctggacgtgc agcaacgaag
      361 ctttcaccga cgccacggga tgggttccgc gaacccgcct gggccagggc gtcgctgcca
      421 tgttcgcccg ttgaacgacc ctgagaggta ttgcacttgt ccgctagcca gaccgacatc
      481 atcgtcacgc tcgcagaact gctctgcgcc cagcttccgg agccggtagc cgtcgccccc
      541 gatacggatc tcatctccga tctggctctc gaatcggtcc agatcatgga gttcatcgtc
      601 gaggtggagg atcactacga catcgccatc agcctcgaag cgctggccga gatccgcaac
      661 ctcgatcagc tggcgcgtct ggtcatcacc gccctggacg cccaatgagc ctcctggaga
      721 agttcgatgc cctggagact acccgccagg agctcctcgg ccacgggacc aacccgaccg
      781 acgtgatcat cgagagggtc atctcggcaa cggaatccgt catcggcggc aagcccacga
      841 tcatggcggg gacgaacaac tacctcggcc tgactttcga cccggaatgc atcgccgccg
      901 gcaagcgggc gctggatgaa tcgggcaccg gcaccacggg atcccgcatg gccaacggca
      961 attacgcttc ccatggggag ctggagcggg agatcgccga cttctacggc tacccctacg
     1021 ccatggtgtt ctcgaccggc tacgcggcaa acatcgggac catggcagct ctggtgggtc
     1081 cgggagacgc cgtgatgctc gatggcgacg cccacgccag cctgttcgac ggcagcaaac
     1141 tttccggcgc aaccgtttac cggttcaagc acaacgatgc cgatagcctg gacaaacgcc
     1201 tgcgccggct aggtgacgtc gccagccgca cgctggtcat cgtcgagggg ctctacagca
     1261 ttctgggaga ccgcgctccg ctcaaagagc tggcggaggt gacccgcgcc cacgatgcct
     1321 gcctgttcgt cgacgaggct cactcgcttg gcgtgctcgg agaaaccgga cgcggacggg
     1381 tggaggaaga ccaggtggaa gccgacttcg tggtcggcac cttcagcaag agcctgggtt
     1441 ccacgggcgg cttctgcgtg agcccccacc gagcgctgac cctgtttcgc tatgccagcc
     1501 gcccctacat gttcacggcg tccccatgcc cgtcagtcat cgccaccacg cgggcggccc
     1561 ttgctcagat gcgcaaacgg cccgagctgc gcgcgtcgtt gtggaggaac gctgaaacgc
     1621 tctacagcgg cctggcccag ctgggattca ccctcggccc cgagccgagc cccgtggtcg
     1681 ccgttcgcat gacctcccgg gaacaggcac tggcgaactg gcagcagctg ctggacaacg
     1741 gtatctacat actcctcatc ctgcccccgg ccgcgccgga cggcaccgcg ctgcttcgct
     1801 gcagcgtcag tgcagcccac acgccggaac agattcaacg catcatcgac gcgttcaggc
     1861 agctgccccc ggatcctggt cagcccgcgt aagcaccagc gcgaccatcc ctgccagcgc
     1921 aatcgctccc gagcacgcag cggcgatgcc ggcacccgcc agccccagcc agggcacgag
     1981 gacggcaaaa gcagaaacgt ggcatacggc ggtgacgatc tggacccgca gcgcgccggt
     2041 cgccgcgccc atggcgtaag ccgccggcct gagagccgca ccgatcagct ccaacgcacc
     2101 agcgaccatc agccacacga gcagcgccgc gccgtgcacg tattcctcgc ccacgagcgc
     2161 ttcgagaagc tcgccggcat aaaggcccac caggccgacg accacggcgg cgatcagccc
     2221 gccgtaaagg ccgaggcgcc agtagagcgt cttgaaccgc gggtcgcgct cccgccacag
     2281 acgggcgaag tccgggaaca cggcctgacg caccacgatc acgggcttgg ccagcacatc
     2341 ggcaaactcg cgggcgacgc gaaacatccc ggcacccgcg gggccaagca gaacgcctgc
     2401 cagcaggatc aggccgtgcc gctgcgcaag atcgaggttc gactgccagt agagcgtgcc
     2461 gacgaagccc cacaaccctg gaaatcgtcg tttcgcgccg gcaagggtgg gacgtccggg
     2521 ccggactcct cgccttttga gctccagcca gccacgcacg ttcatgaaga gatgctcggc
     2581 ggcaaacgcg acggcccaaa ccatcaggta accaagcaag cctccctcag ccaggtacac
     2641 ggcggctacg ccggccagcc ggatgagcgg ccccacgttc tgctggacgc ccatggcatg
     2701 aaatcgccct gctacccgga gcacgcctcg cgcggtcccc gagcccgatg ccagcagaac
     2761 caggccgtag agaacggccg gcattgccag ctcctccggg agccccaggg cggggcccgc
     2821 gagcagcatg acgagcatca tgcacagcgc ggcaaggctg gcagtggcga catccagggc
     2881 aagagtgaag cgcagcaggt ggttcagctc ccgatggtcg tcgtcgtcaa acggcgcgac
     2941 gccgtatcgg accaccgtgt cagacggttt gaagttgagc aggccgcgca ccaccacggc
     3001 cgtggtgtgc acgaccacga ggagcccgaa ctctaccggt ccgagaaagc gggcaaccag
     3061 ggtcagcgaa accacggaca gcacaccggc cacggcacgg ccacccatca gcacggagaa
     3121 gttcttcagc gccctgcgaa cgggcgagtc ggagaccgac tcgttcacgc gcgagctccg
     3181 ccccgcgccc gcacggcgga cccggcagtg tgaccattca ccggaacgcg cctctgcgat
     3241 agagccgcca gaccagcggc caggcaaaga tcagcgggta gcgatgctgc cgggcgcttg
     3301 cttccacccg caccccgctg tgccgctcca ccttccagag tatgtaatcc aacgcgtcgt
     3361 cgaaggtgag cgcgcctttg acaagccggc caaccgatag aaactttccc agcgcgatca
     3421 cgcacgccca ttccacggcg gctgaaaccc gcggcctgac ggcgcggtag cggcctcccg
     3481 cgcagaccag gtgccggccg tcagccacgg cggccagcat gttgtcgtag tagtcgggat
     3541 cgtgatcgat gatcgagtca cttcgcccag ccgactcgga ccgaagctcg gaacggtagg
     3601 tgagcgcaaa gccccgggcc cagaattccc ggctcgtgaa atcccctctg agcgtgggag
     3661 cgacgcgttt gacaaacgta cgcacacatt gctcgataat ctgaacgagt gccgcctcat
     3721 cggcgggcca gcgtgcatag acgatgcggg ccggctgggc aaagcgcgcc cagaagtagg
     3781 gatgcagccc cgaggcggcc tgcctgcgca gctgtctcat ggtcacgacg gcgtactttg
     3841 cccgaacacg ctcggcgccc tcacccaccg caaggtagta gacgttgggc ggcaggaccc
     3901 gcgcgcccag ggccgaaacc ggcgagccca gggcgccgcg gtagccgtcg acgaccacgt
     3961 agaaatccag cagggtatcg cgcacgccgc gcagataaga tccgtagagc agcacggcct
     4021 gaaggtgctc gccacaccgg gcacgcagtt ccgagagcat tcgttcgaca ccttgatcgt
     4081 tctcgagacc ggcaggtgcc gtgcgctcga cgatgactga cctggcgtca cgcatcaggc
     4141 gaggtgacta gcgctgcaca taggctccgc gggtccgcga aaatgtccgg aacgcgcggg
     4201 ggtatgcggt cgccgccgac ggctcggtga gccaagagga cagcggcccg tcgctcatgc
     4261 gggcaagcgt cgcctgcaga agcctgatca tcagcacgac cgtggtgccc accgtccaga
     4321 acaccaccgc caggaacgcg ccgccaggac tgcccagcag catcgccacg agcatcagga
     4381 tgaggcaggg attgcgcctg gccgtgacca gtcggaagta ggcgtcgaac ggcctccagt
     4441 cgaacaggct gcaggagccg aggccgtgaa aggcggcttc gatgagccgt ccgccgacgt
     4501 agccggcaac gatccaccag tagtactcca gcagggggac gctgaaaccg ctatcgtcca
     4561 gcgacatgcc ccacagcacg taccagaacg gcggatgcac gatgtccatg ccgtgatcga
     4621 ggacgtgccc gatgcggctc gagctcacgg tgacccgggc aagcttgccg tcgaccgtat
     4681 cgagaaaagt catgaaccat ccggccgcca gacccagcca gtagtcgccc tggtagaagg
     4741 caaaccccgc ataaacgacg agcaggagac ccgccagcgt gaccatgttg ggcgtgattc
     4801 ccaaccgcgc gcagacccgg accgcgtgac gcgcgggtct gggccacagc cacttcgtca
     4861 ccaggtcggt gatccctttg taggaacggc catacagccg gtcctcgagc tcgccacggt
     4921 cttcttccgc gatcggcagc agaagcggct cttcggcctt tcgcaggcga tcctcatacg
     4981 cggccaggtc agccggcgcg agcgtcggta gatcgtgatc cgtcgtcgcg ccttcgagca
     5041 gcggaaccag ctgcgcggct tcccggcccg tgctcaccag ggcggccgcc ccgccgtcgt
     5101 tggggcaaac cagaaccgca cgcccgcgct cggccagggc tttgatcgtt ctcgactcga
     5161 agaggtaatc cgcgcgaatg cagatcaccg tagcgtcctc cggcacggtg ccgagctgat
     5221 ccaccaggac cgcgttggga agctcgacga cctgtcggcg caggcgctca cgtgaggtca
     5281 gtccccaaag ctcaatatcg gagtcgccga gcaggtgtgc gtaaatgctc atgttatagt
     5341 tggaaattca ctgcgcactt ggggatcctg ggacttgcgg cgggcgttta caggccaagc
     5401 cgccgacggt cgcggattat aggcatgccc cagttgctcg gcaaagggcg gccatgcgtc
     5461 taatccatct cagcgatccg catctttcat ccctgcacgg cgtgaagctt cgcgacctcc
     5521 tcggcaagcg gacgctcggg taccagtcct ggtaccggaa gcggcgccat cagtacacca
     5581 tggacacgct tcgcagggtg acgcgcgcgg tccgcaacga ggccgcggac gccatcgtgg
     5641 tcactggcga cctcgtgcat ctcggcatgg cgcaggagct tcgccaggcc agggagtggc
     5701 tcgagtcggt gggcggcccg gatcgggtgc tggtcattcc cggcaaccat gattgctacc
     5761 atcgccagtc gtgggccctg gtcaaagaga cgatcggccc ctacctggcg ctcgagaagg
     5821 acacggcgga cgcgcgcgac ggctaccccc tggtgcgacg ctccggcgag gcgagcgtca
     5881 tcgccgccgg cactgccgaa cctactgcct ggtggtccgc ctcaggctcg ctgggcgccg
     5941 cccagacaac gcgcctggag gacgcgctga taaaggcgcg ggagacgttc accttcctcg
     6001 ccattcacca cccgccgctg ccaggcatgg cgccgcgccg aaagtcgctt cgcgacgcgc
     6061 acgccctggc agagctcctc gaacggcacg cgcccgacgt ggtcctgcac ggccatctgc
     6121 atgccaatca ggaaaactcc gacacgccca cacgcatatt ctgcacggcg ccgccgtcca
     6181 gcgagcgacc gcgggcgccc gcttcctacc gcgtgttcga cgtcgtcgaa gacgcgtccg
     6241 ggtatcgcgt cgtcatgcgc ctgaagtcgc tgatcggcga ggagatggtg gtcgcagacg
     6301 agcagtcgtg gacctccggc cgatcactcg acccgggtga gcgcgacgat gctgccaagc
     6361 agtagcgcgg agtccggcag catcgccagg ggtatgaccg gaagctcgta gagcagtgcg
     6421 agctgagcgg acgtctgctc gaccagataa aaaccgatgc cgaccgtggc gccgaacaac
     6481 accctggcgc ccgctggccg agcccgcacc gaaccgagaa cgaacggcaa gccgagcaga
     6541 cacatggcca tcagcgcaat gggcgagctg agcacctgcc acagcctgaa gcggtagcga
     6601 tgagcgttga gacccgacgc ctccagatat tcgacgtacc ggtaaaggtc gcccagggtc
     6661 agggtttcca tcggcgcgat gagggaggcc atctggtccg gagacaggaa gctctcccac
     6721 tcgtaggtgt cacggccggt gcgcctgacc tctttgccga gaattcggcg ctcttcgaca
     6781 ccgtgaagca gccactggcc cggcccgatc acgtccgcgt actcggcctg cagcacgcgc
     6841 tgcacgacgc cgcggtcgcc gagatcgtag atctcgacat cagcgggaat catgccgaaa
     6901 cggacgtccc ccaccctgat gaagtgagtg ccgtcgcggg tccagaactc ggtggtctcg
     6961 ccgccacgca tcgtgttgac cagcgacttc gcctgaagct ccttggcctg ttcctcgaac
     7021 cgaggcacca cgaactgctg cgccagcagc acgacgtaag acaacacgat ggccgccagc
     7081 gcaagcggat aggccatccg ccattgggat agaccaaccg ctcgcagcgc cgtcagctcc
     7141 tgttcctgag ccaggttgcc gagcgccatc accccaccga gcagcaccgc caccgggaga
     7201 agatcgatgg cgattcgcgg aagcgtcagc agggcgacgc ggatcgcgtc cgcttcctcg
     7261 tactgcccct tgccgacgtc ctccagctct tcggcaaggg tgacgaaacc gaacagcccc
     7321 agcagcagca acagaacggg taccgcgccc agcagcaaga cgcctacaag gtagcggtcc
     7381 acacgcttca cgcgcttcgc ccccgtcgtc tgaaccaccg ggcccatggc acgtaggcca
     7441 gcacgatgcc gcctgcaaag gtcgccggga cccaccacaa cgtcgccgcc gtgccctgct
     7501 cgacccaggt ccttccgatg cccagaacgt tgaagtacac ggcgtagatg accacggcaa
     7561 ccgtgagccg ggcaaagcgc ccctgacggg gccgggaccg tccgagcagg acggccagca
     7621 acgccagcag aatggtcgaa acgggcgccg acaggcgcca ttggtactcg gcctgatcca
     7681 ctagcgcgtc ggattctctg aggacgaacg agttctggga tttcggcttg tagcccacgg
     7741 gctgcaccgc cgccaccgga acgaatatgc gcagctcgcc gaagatgccc agaaagtcgg
     7801 cgccctgcgc agttttcctg tagacctgag catcgcgaag gtcaacctgg tggttcttgt
     7861 cgctgacata ctcgctcacg gtaccgctgg gcgcggagat gatttccaga tcatcgccgt
     7921 ccagcgcgcg cacgaacagc ccctgaatct catcgtcttc ggccttcagc tcctcgatga
     7981 agacggtccg ggcattgtcg tcgtacaggt agaatcgccc gggtttgatg cgctcgagct
     8041 ccgaggtcgc ttccgcttcg acctccagcc tgtagaggtg ctcgtacgcc cagggcctgc
     8101 ccaccaggga gaaaacggcg acgacgagcg caaagcccag ggcgacgctc atgatcggga
     8161 aaagcacctg acgctcggca aacccgaacg agcgcagggc cgtcatctcc cagtcgctgt
     8221 agagtcgccc gagggcaagc accagggcga cgaagaggct gaccggcagc agcacttcct
     8281 gagcgatgaa agacttgagc gccgtaagct gcagcacctc cgacgtccgc agcaaaccgt
     8341 cggcagcctt gtcgaggaac cgggtcatgc tgtaggtggt gaatatcacc gtcaacacgc
     8401 cgccaaccgc gaaaaacggc agcagaagct cgcgaacgag gtaccggttg atgatcagtg
     8461 ctctaaccct ctcccgaact gcaggccagc ccgtcgccgg atcctgatat catcgacccg
     8521 gtttcccgac gaccccggcc gagctccatg ctgaaaagat ttttcgccgc ggaactggct
     8581 tcggccattg cgctggcgtt ggtgcagctc tcttgctcgg catgggccgg cgagctgccc
     8641 gcgctggacg atcaagatac cgtagttgtc accgccgacc aagcttggga gtccgaggac
     8701 ggcgactcgt tgagctttgc cggcaacttc aacctcaacg cccccgactt ccacgtcgca
     8761 tccgacacgg cggtcattca cgggcctttg gaggatccca accgcatcgt cgcggagggc
     8821 agccccgtgc gcttctgggt tgccgatccc gattccggga acaggactca cggaaccggc
     8881 aggcggctgg aatacgatcg gcaaagggat ctggtccggc tcgaggggga tgccgtactg
     8941 gaagacgacc ggaccgtgat gcgaagttcc gagctcgaat acaacaccgc cacgaaacga
     9001 ctcgtcgcgc aaggatcggg cggcgtggag atcgttacga acccgaaccg cgactgagca
     9061 cccggctcag ctccaggcct cacgcaactg cccgagcagg gcttcacgtt cggccgcgtc
     9121 gagctcatga aactccccca gggccggcgg tccgggccag ccgtggtcgg ctatctccac
     9181 cgtccccagc agacgggcgg cctcgtcgcg gggaatgacg tgatagtgca cgtcggggtc
     9241 gaccatcatc agcatcaggt agttgatcct gtcgtagctg tacaggctcg acagggcccc
     9301 ttcggccgcg gctatcacgt cgtgaagctc ggtgaacgcc gccctgctga cctcggagaa
     9361 tcgggtgaca ggctctttgc agaccaggac cagggagccg agagagacct gctgcggccg
     9421 gacgagaacc agccagtgac cgtactcgcg caccagcgtg tccggatagc cgaatttctc
     9481 tgccgtggcg ttcaagtcaa tccgccgccg cccgcgaggc gggcttgcgc caggcagtga
     9541 ccacgcccgc cgcgttgtcg agatcgtccc ggtcgtcgac ttcgcaccat gacagaccgt
     9601 gaatgtcgca aaccccaacc tcgccgctct gagcgagctg atcaacggca gagaggtacc
     9661 agagcttcaa acccatgtcc gaacgcatca gcgactccac cttgctcacg aacttggcag
     9721 gacccagacc cctgaatgcc atcatgccga tggactcacc gttcacgtcg tccagctcga
     9781 gtcgcttgcc cacacgccga agacgcccgt cgctgaccac gactttcatg tcgtcctcgt
     9841 cgtagtgatc cttacggtcc accacgagcg taacgggcgt attcgcggga gatgccagca
     9901 gcttctgcag gatggccgcc tcgaacagcg tgtcgccgtt gatgatgacg aaggggtcgg
     9961 tcatttcgct gcgcgcgacc cagcaggtgc caagattgtc gctcgcatcg aagaaggggt
    10021 tgtgcagggt tctcaccggg accatgctgc agcggctgac caggtcatcg acgatgtcgg
    10081 catggaaacc ggtaacgacc acgacctcgt cgatgccgca ttgagccacc tcgccgagct
    10141 gccattccag caggctctgg ccggcgatct cgacgccgca tttgggcgtc ccggccgtca
    10201 gcggcagcag cctgcggccc tgaccagcgc tcagaatgat ggccttcact agggtaccgg
    10261 tatcgggaaa acgcgaaggg tcggggtttt caccgcgagg gtcaagactt tccgcccagc
    10321 aggccgtgca gatggtctcc cagccgcagc gccagggcca gaatcgtcag ggtggggttc
    10381 gcccagccgg cggtggcaaa gacggagctg ccggcgacga acagattgcc gtagccgtga
    10441 acgcgcccac tcccgtccac cacaccgtgc cgaggatcgg cactcatgcg agtggtgccg
    10501 atgtggtggt acccgccgat ggggtggttg cccacggtcg ggtcaacggg ccagtcattg
    10561 cctggctcgc ccagccagga actcgcctcg acggaaccca ggcccagccg ggtcagctcg
    10621 ccgtccagca gccgcaccat cacctggatc gtgcgcttgt ccagctcgcc gagctgccag
    10681 ctcagggcag cccggggcat gcccagggcg tcacgctcgc tgcccagggt gatgcgactg
    10741 tccgggttcg gggcctgctc ggcccggatc atgagataaa ggcggcgcat gccgaggccg
    10801 gcccgcagcc gctcgaaggg aacccgggca agccgctggg ccgagctgcg cacggccctg
    10861 taggcgtgcc acagggtacg cccccgccgg gtcggcgcca tgccgtgctt cagggtctga
    10921 tacatctgcc gcgttaccgc gaccccgcgc tccggatccc gctgcagact gagcgtgaac
    10981 gcagaattga gtatcccctc gcgctcctga gcggccgcag acgggacgag aacgggcgcc
    11041 agatccggtc cgtgactgcg aaagcgcttg cggaaggccg cccacaaggg gaacgcgccc
    11101 ggccccgaga cccggcccgc gcggccgtgc gggtgctcca tgaagtagcg cccgacctgg
    11161 tcgttgccgt tgccgacccc ggccgcctcc acatcgcggg aggccagcag gagacgggca
    11221 ttttcgatcc cgccgcaggc gagcacgtag tgccgcgccg tcacccgccc ctcaatcccg
    11281 gaaagcgaag acactttcag atgctcgatg ctggccgcgt tggccgccgc ctgaatgtgg
    11341 gtcacgttgg cgtgcagaac cagagtcacg gcagcgctgc tggccaggtc ctgcacccga
    11401 cgggcggcga agcgttcccg cacgtcgtcg aagcgccaga atcgcgtgct gaccgcgtcc
    11461 gggtcgaagg gcggcacccg ttcgccgacc tcgtcccaca acgattcgtc gtaacgcacc
    11521 tcgccgagct cgagctcgtc gtgcacggcc gcgtagtaag gcgtgagctc cgcattggtg
    11581 atgggccagc cgctgtgcgg cacccacggc ctgcgccgga aatcgatgtc gtcgagcaag
    11641 gcgcagcgcc cgccccagat gttggtggtg ccccccagaa agcgcaggcg ggcatcgacc
    11701 aggtcatagt aggtcatgcc gtcgctggtc ccggcgtaga gatcctgggt ggactgctcg
    11761 aaatccaggc cgccgctctc cagcaggcag atggagtgac cgagctccgc caggcgccgg
    11821 gccagcgcca caccggctgc gccggcgccg gcaatgcaga cgtcggcctg gaaacgctcg
    11881 ccctggggca gcttttcgag atcgagcagc acgactaagc ctcgcgcctc gcccggttcc
    11941 agtcggccag gttcttcagc ggatccttca cgtaggcgct gccgcggatg agcgtgacga
    12001 tcagtcggtt gaaccacgtg cccaggcggt ccgacggccg gtccacatcc agaaacagca
    12061 cgatgcgtgt ctcgtccgtg tcgtttcgca cctcgtgctc gaacgtatca tcgaaaagca
    12121 gacagccgcc cgcctcccag tggcggatct ggtcgtcgac gcgcagccag caccgttctc
    12181 cgtcacgggg cacggcgaga cccaggtggc aacgaatcac cgcacgggta ggccccttgt
    12241 gcggcgggat gtggtagcgg ggcgcgagga tggagaacca ggcgttctgc agacccggaa
    12301 ggcattcgat ggtcgcagcc gtgaccgggc atcgccggca gttttcttcc aggcggatac
    12361 cgaacacgta gaaggcgaac gtcttccagt tgtcgccctt ggagatgcgc ttctggtccg
    12421 gcgacagctg atgaaacgcg gg
//
LOCUS       DBNHQR010000041         3022 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1714101, whole genome shotgun sequence.
ACCESSION   DBNHQR010000041 DBNHQR010000000
VERSION     DBNHQR010000041.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1714101"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="AC16VR_00985"
     CDS             <1..512
                     /locus_tag="AC16VR_00985"
                     /inference="COORDINATES: protein motif:HMM:NF014667.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR6009753.1"
                     /translation="ARVVDAQGVIGRLHEMLGDKVVVLSFIYTRCNDVNGCPLATHVL
                     GKLQGAITSAPDLQGKVRLVSFSFDPSHDTPAVLRQYAGHFRKPGFDWRFVTVANAAD
                     LTSVLDAYDQWVVRDIDADGEFVGTLSHILRVYLIDRDRQIRNIYSISFLHADTVAND
                     IRTVLLDGG"
     gene            complement(575..1834)
                     /locus_tag="AC16VR_00990"
     CDS             complement(575..1834)
                     /locus_tag="AC16VR_00990"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6009754.1"
                     /translation="MSEALAPIDLTTADPYSVPLDQIDMSQAALFQTNTFGPYFERLR
                     REDPVHYTADSFFGPYWSITRFDDIVYVDKHHELFSSEDGITLADRQQDFATVNFIAM
                     DPPKHDDQRKAVQGVVAPRNLAKMEALIRGRVADILDSLPLGETFNWVDRVSIELTTQ
                     MLATLFDFPFEERRKLTYWSDMATSSELAGGPTPEPVRREALMECLEAFTRIWHERAS
                     QPPDESVDLISMLAHSDDTKDMLNRPMEYLGNLILLIVGGNDTTRNSITGGVLALNQN
                     PGEYDKLRQHPELIPNMVAEIIRWQTPLAYMRRTATQDTELGGKLIRKGDKIAMWYVS
                     GNRDETAIDQPEEFIIDRPNARHHLSFGFGIHRCMGNRLAEMQLRIVWEEILKRFHKV
                     EVVGEPVRVQSSFVKGYSQLPVRLHPV"
     gene            complement(1859..>3022)
                     /locus_tag="AC16VR_00995"
     CDS             complement(1859..>3022)
                     /locus_tag="AC16VR_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009755.1"
                     /translation="ALEDVVRDYPDDLNAQALYAEALMDLYPWDYWDDDGSPRMHTGA
                     IVAALEKVMEADPDHPGALHLYIHVMERFTPELAEPAADRLGPLVPVAGHLVHMPSHI
                     YLRVGRYHDAVAANRRAALADEDYIAQCNAQGFYPAAYYPHNIHFLWYSAMMEGRRAL
                     AVESAEKLRSKVPVDMARQFGSIQAYLAVPSYTFVRFGMWQEALAVPEPEEDLVIAAF
                     MRHYGRGVAHAALDDTGSAKAELAAMEAILASGEFRAQMDRQGDIGDILASIASSLVR
                     ARIARADGDVDAEIRHLRDAVASQDSLPYSEPPYWHYPVRQSLGSALLRLGDADGAAA
                     AFTEDLGKFPKNGWSLHGLAEARRALGKPTDELEAAHREAWQYADIEPTAGAE"
BASE COUNT          498 a          946 c         1019 g          559 t
ORIGIN      
        1 atgccagggt ggtcgatgcc cagggagtta tcggacggct gcacgaaatg ctcggcgaca
       61 aggtggtcgt gctgagcttc atctacaccc gttgcaacga cgtcaacggc tgtcccctgg
      121 cgacccacgt gctcggcaag ctgcaggggg ccatcaccag cgcccctgac ctccagggta
      181 aggtgcggct cgtaagcttc agcttcgatc cgtcccacga cacgccggcg gtcctgcgac
      241 agtacgccgg ccacttccgc aagccggggt tcgactggcg cttcgtcacg gttgcgaacg
      301 cagcggatct cacctcggtg ctggatgcgt acgatcagtg ggtggtgcgc gacatagacg
      361 ccgacgggga gttcgttggc acgctgtcgc acattctgcg cgtctacctc atcgaccggg
      421 acaggcagat acgcaacatc tacagcatct ctttccttca tgcggatacc gtcgccaacg
      481 acatccgcac ggtgctgctc gacggcggat aaccgcaacg ggcaccccag gctgaccccc
      541 ctgacacccg ccgttccgag gggccgcgcg caaactagac cggatgcagt cgcacgggca
      601 gctgcgagta tcccttgacg aagctggact gaacgcgcac cggttcgccc accacctcaa
      661 ccttgtggaa gcgtttgagg atctcctccc agacgatgcg cagctgcatt tccgccaggc
      721 ggttgcccat gcagcggtga atgccgaagc cgaaggaaag gtgatggcgg gcgttgggcc
      781 gatcgatgat gaactcctcg ggctggtcga tggccgtctc gtcgcggttt ccggagacgt
      841 accacatggc gatcttgtcg cccttgcgga tcagcttgcc gccgagctcc gtgtcctggg
      901 tggccgtccg gcgcatgtag gccagcggcg tctgccagcg gatgatctcc gccaccatgt
      961 tgggaatgag ctcgggatgc tggcgcagct tgtcgtactc gccgggattc tggttcagcg
     1021 cgagcacgcc gccggttatc gagttgcgcg tggtgtcgtt gccgcccacg atcagcagga
     1081 tcaggttgcc gaggtattcc atcggccgat tgagcatgtc cttcgtgtcg tcgctgtggg
     1141 cgagcatgga gatcagatcc acgctctcgt ccggcggctg gcttgcgcgc tcgtgccaga
     1201 ttcgggtgaa ggcttccaga cattccatca gcgcctcacg ccgcactggc tcgggcgttg
     1261 ggccgccggc cagctcgctg gacgtggcca tgtccgacca gtaggtgagc ttgcgccgct
     1321 cctcgaaggg gaagtcgaac agcgtggcca gcatctgggt ggtgagctcg atggaaaccc
     1381 ggtcgaccca gttgaaggtc tcacccagcg gcagcgaatc gagaatgtcg gcgacgcgtc
     1441 cgcggatgag cgcttccatc ttggccaggt tgcgcggcgc cacgacgccc tggacggcct
     1501 ttcgctgatc gtcgtgcttg ggcggatcca tggcgatgaa gttgacggtg gcaaagtcct
     1561 gctggcggtc ggccagggtg atgccgtcct cggacgagaa gagctcgtgg tgcttgtcca
     1621 cgtagacgat gtcgtcgaag cgggtgatgg accagtaagg cccgaaaaaa ctgtccgcgg
     1681 tgtagtgcac cggatcttcc cggcgcaggc gctcgaagta cgggccgaag gtgttggtct
     1741 ggaagagggc ggcctggctc atgtcgatct gatccagggg caccgagtac gggtccgctg
     1801 tggtcagatc gatcggtgct agggcttcgg acatcacgaa cctccggttt tgaactcgct
     1861 actcggcgcc tgccgttggc tcaatatcgg catattgcca cgcttcccgg tgggccgcct
     1921 ccagttcgtc ggttggcttt cccagagcgc ggcgggcttc ggccagcccg tgcagggacc
     1981 agccgttctt cggaaacttg ccgagatcct cggtgaaggc cgccgctgcc ccgtcagcat
     2041 cgcccagccg gagcagcgcg gagcccagcg attggcggac ggggtagtgc cagtagggcg
     2101 gctcgctgta cgggagcgag tcctggcttg cgacggcgtc gcgcagatgc cgaatctccg
     2161 cgtccacgtc cccgtctgct cttgcgatcc gggcgcgcac gagggacgag gcgatgctgg
     2221 cgaggatgtc cccgatgtct ccctggcgat ccatctgggc ccggaactcg ccggatgcca
     2281 ggattgcctc catggccgcg agctccgcct ttgcggaccc cgtatcgtcc agggcggcgt
     2341 gagcgacccc gcggccgtag tgacgcatga acgcggcgat caccagatct tcctcgggtt
     2401 ccggaaccgc cagagcttcc tgccacatgc cgaagcgcac gaaggtgtag gacggcaccg
     2461 cgaggtaggc ctggatggac ccgaactgtc tggccatgtc gacgggcacc ttgctgcgca
     2521 gcttctccgc tgactcgacg gccagtgccc gccggccttc catcatggcc gagtaccaca
     2581 ggaaatggat gttgtgcggg tagtaggcgg ccgggtagaa gccctgggcg ttgcactggg
     2641 caatgtagtc ctcgtctgcc agggcggccc ggcggttggc ggctacggcg tcgtggtagc
     2701 ggccgacgcg cagatagatg tgcgacggca tgtgcaccag gtggccggcc acgggaacca
     2761 gcggaccaag gcggtcggcg gcgggctcgg caagctccgg tgtgaaccgc tccatcacgt
     2821 gtatgtacag gtgcaacgcg ccaggatgat cgggatcggc ctccatgacc ttttccaggg
     2881 cggcaacgat ggcacccgtg tgcatccggg gggatccgtc gtcgtcccag taatcccagg
     2941 ggtaaagatc catcagcgcc tcggcgtaga gcgcctgggc gtttaagtcg tcgggatagt
     3001 cgcgcaccac gtcctccagt gc
//
LOCUS       DBNHQR010000042         2813 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_954365, whole genome shotgun sequence.
ACCESSION   DBNHQR010000042 DBNHQR010000000
VERSION     DBNHQR010000042.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_954365"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..445)
                     /locus_tag="AC16VR_01000"
     CDS             complement(125..445)
                     /locus_tag="AC16VR_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009756.1"
                     /translation="MPIHIAKYERRDHTDRTDLALAALEACRSARATAGVNSSRFYWV
                     NPDEIAIVTDAEPGAWGQGSGNQPEPRAMKAMFELADLARNTSVETWADARSGEETFK
                     ISQS"
     gene            complement(545..1057)
                     /locus_tag="AC16VR_01005"
     CDS             complement(545..1057)
                     /locus_tag="AC16VR_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009757.1"
                     /translation="MPALDDRSLGWLRYLHRKATTPDNWDRDGRPHPDRYRSIAPAGL
                     WGEYDVSGWTANGIEPYGMQPDPVAADGMLFYKGFFNLLMSLHRYVSGSTKWDQPFEM
                     IRDEANTFEWAHSGADLYYDPVADEHVGTGWSMQTPGRETVPLTGENNRVNVQLTTDN
                     RTVIVRRSVG"
     gene            1138..1863
                     /locus_tag="AC16VR_01010"
     CDS             1138..1863
                     /locus_tag="AC16VR_01010"
                     /inference="COORDINATES: protein motif:HMM:NF018777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD domain-containing protein"
                     /protein_id="HIR6009758.1"
                     /translation="MSDEGRVPATDGVRMMFLIRRRVGSSREELVAHWFANHMPAVIA
                     AMQGTARYYMAVLYDADASGSYPWDGVAQILVDEAMPTPSEPFGTTPTDSFQEHAEPY
                     WPWATTEYVVIDGSENLPVRPLTLNDPFPTTRSGFFKISFLVKAKPGTDHGAFFDHWL
                     DVHVPNVAGTMDEVGGFRYVVSHSLNPERAPYAGMAELYFHDEDGWRRYKAAIQPDGM
                     ERWAADEGTLVLRSHTEMIGIPG"
     gene            complement(1912..>2813)
                     /locus_tag="AC16VR_01015"
     CDS             complement(1912..>2813)
                     /locus_tag="AC16VR_01015"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6009759.1"
                     /translation="ISNGATLVIHEDFHPGHVIQALSDGGITSATLVPAMIQACLVSV
                     PDAGERAYPSLEYMAYGASPIAEETLRQAMAVFKCNFAQVFGMTETTAMATVLGPAEH
                     RRALAGEPALLKSAGRAALGTEVKIVDEEDREVDRGTIGEVIVRGPQVMMGYWNLPEA
                     TEKSLKDGWMHTGDAAYMDDEGFIYIQDRIKDMIVSGGENIYPTEIENALFEHDAVAD
                     AAVIGIPSEQWGESVLAFLVLKDGQTVTPEEMIEFCRPRLAGYKIPRRIEFLDAIPRN
                     ASGKILKKDLREPFWEGVERRVS"
BASE COUNT          427 a          920 c          928 g          538 t
ORIGIN      
        1 gagtagtagc gttccggcgt aatgggatcg ccgcgcaggt tgcgcggatc ttttccgtcc
       61 atggcaagtg gatgcttgac gatgtcgttc atcggatatc ccctcgtttt ggctccgggt
      121 gtgatcagga ttgtgagatt ttgaacgtct cctcgccgga gcgggcgtct gcccaggttt
      181 ccaccgacgt gttgcgcgcc agatcggcga gctcgaacat ggccttcatc gctctcggct
      241 cgggctggtt gccggaaccc tggccccagg cgcccggttc ggcgtcggtg acgatggcga
      301 tctcgtccgg gtttacccag taaaagcggc tgctgttgac ccccgccgtg gcccgggcgc
      361 tgcggcaggc ttccagcgcg gcaagtgcca ggtcggtacg gtcggtatgg tctcggcgtt
      421 cgtactttgc aatgtgtatt ggcatggttt tcccctgatc ctttctgtcc cgtctccgga
      481 taatggcccg aatgaaggcg gtttggaacc ccggggcgaa cgcccggcag ggccaggtcg
      541 gacctcagcc gacagaccgg cgcacgatca ccgtgcggtt gtcggtcgtc agctgaacgt
      601 tcacgcgatt gttttcgccc gtcaggggca cggtctctcg gcctggggtt tgcatgctcc
      661 agccggtccc cacgtgctcg tcggccaccg ggtcgtagta gaggtcggcg ccggagtgcg
      721 cccactcgaa ggtgttggcc tcgtcgcgga tcatctcgaa cggctgatcc cacttcgtgc
      781 tgccggaaac gtagcggtgc aggctcatca gcaggttgaa aaagcctttg tagaagagca
      841 tgccgtctgc ggccacggga tccggttgca tgccgtaggg ctcgatgccg ttggccgtcc
      901 agcccgacac gtcgtactcc ccccacaacc cggccggggc gatggaacgg taacggtccg
      961 ggtggggtcg gccgtcgcga tcccagttgt cgggtgtggt ggccttgcgg tgcagatagc
     1021 gcagccagcc taacgagcgg tcgtccagtg caggcatcgg cagctcccgg tccaatgcgg
     1081 gtaagctggc agcaatggtg ctacgctgca gccaagcgac gggtggggag gatgctcatg
     1141 tcggacgaag gccgggttcc cgcaacggac ggggtgcgca tgatgtttct gatcaggcgc
     1201 cgggtcgggt cgagccgcga ggaactggtc gcccactggt ttgccaatca catgcccgcc
     1261 gtcatcgccg ccatgcaggg aaccgcccgg tactacatgg ccgtgcttta cgacgccgat
     1321 gcctcggggt cctacccctg ggacggcgtg gctcagattc tcgtggacga agccatgccg
     1381 acaccctccg agcccttcgg caccacgccc acggacagtt ttcaggagca cgcggagccc
     1441 tattggccct gggcgaccac ggagtacgtg gtcatcgacg gctcagagaa cctgccggtg
     1501 aggcccctga ccctgaacga cccgttcccg accacgcgct cgggcttctt caagatctcg
     1561 ttcctggtca aagcgaaacc cggtacggac cacggcgcgt tcttcgatca ctggctcgac
     1621 gttcacgtgc ccaacgtggc ggggacgatg gacgaggtcg gtgggttccg ctacgtggtc
     1681 agccacagcc tcaaccccga acgggcgccc tatgccggca tggccgagct ctactttcac
     1741 gacgaggacg gctggcggcg ctacaaggcg gccatccagc ccgacggcat ggagcggtgg
     1801 gcggccgacg aaggcaccct ggtgcttcgc tcccataccg agatgatcgg catccccggg
     1861 tagccggccg ggctgagcgg caggctaccg ggcgcccagc tggaggcccc tctacgacac
     1921 ccggcgctct acgccttccc agaacggctc gcggagatcc ttcttgagga tcttgccgct
     1981 ggcgtttctg ggtatcgcgt cgagaaactc gatgcgtcgc gggatcttgt agccggccag
     2041 gcgcggccgg cagaactcga tcatctcctc cggggtcacc gtctggccgt cctttagcac
     2101 caggaaggcc agcacgctct ctccccactg ctcgctgggg atgccgatca cggccgcgtc
     2161 ggctacggcg tcgtgctcga agagcgcgtt ctcgatctcc gtggggtaga tgttctcgcc
     2221 gcccgagacg atcatgtcct tgatgcggtc ctggatgtag atgaaaccct cgtcgtccat
     2281 gtaggccgcg tccccggtgt gcatccagcc gtccttcagc gacttctccg tcgcctcggg
     2341 gaggttccag tagcccatca tcacctgggg tccgcggacg atcacctcgc cgatggtgcc
     2401 gcggtcgacc tcccggtcct cctcgtcgac gatcttcacc tcggtgccca gggccgcacg
     2461 gcccgccgat ttcaggagcg caggctcgcc ggccagcgcg cgcctgtgct cggccggtcc
     2521 cagcaccgtg gccatggccg tggtctccgt catcccgaag acctgggcga agttgcactt
     2581 gaataccgcc atggcctggc gcagcgtctc ttccgcaatg gggctcgcgc cgtaggccat
     2641 gtactcgagc gaggggtagg cacgctcacc tgcgtccggc acgctcacca ggcaggcctg
     2701 gatcatggcc ggcaccaggg tggccgacgt tatgccgccg tcgctgagcg cctggatcac
     2761 gtggcccgga tggaagtcct cgtggatgac gagggtcgcg ccgttgctga tgc
//
LOCUS       DBNHQR010000043         3094 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_383840, whole genome shotgun sequence.
ACCESSION   DBNHQR010000043 DBNHQR010000000
VERSION     DBNHQR010000043.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_383840"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="AC16VR_01020"
     CDS             complement(<1..868)
                     /locus_tag="AC16VR_01020"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR6009760.1"
                     /translation="MTDLHYLSLAGVCRRMKSGELSASAVTEAILARIEALDSKLNSF
                     ATVMADEARAVAARLDQDRADGKPLGALHGVPVAVKDLFDTRGVVTASGTRVMGERVP
                     DEDATVVRRLKDAGAVIVGKTQLTEGAYGTHHPDVDPPKNPWDGDYWPGVSSSGSGVA
                     VAAGLSFGALGSDTGGSIRFPSACCGLVGIKPTYGRVSRYGAFPLAESLDHVGPMARS
                     VEDAARLLQVLAGADPYDVTTQPDPVPNYAASVADTLPGLRIGVDWDYVSKGVDEPVV
                     ETIREALEIFREL"
     gene            complement(865..2262)
                     /locus_tag="AC16VR_01025"
     CDS             complement(865..2262)
                     /locus_tag="AC16VR_01025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009622308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR6009761.1"
                     /translation="MTDVVVAELIEALGEDCVLTGEDVHSRQAGIWRSDTIQAKALVR
                     PRTTAEVSRTLAICNAHGQSIVPHGGLTGLVESAITGPDDVALSLERMNRIEEINPVD
                     RTMVVQSGVILQVLQEAAEEHQLMFPLDLGGRGSCTIGGNISTNAGGNRVIRYGMTRD
                     MVLGLEAVLADGTVLSSMNQMIKNNAGYDLKQLFIGTEGSLGVVTRAVLRLREQPACR
                     ATLLVSLDEFAKLAQFLKHMDAALGGALSAFEVMWNNFYTLVTTPPAENQAPIPRDYP
                     YYALVEAMGSDDAAVEAALAEAYEKSLITDAVIAQSEAQRLALWALRDSVEHTARYAP
                     IYTFDVSLRISHMESYLAEVNARLEARYQDVNNFTFGHMGDGNLHLVISVGEGGPETR
                     RAVEACVYEPLAAIQGSVSAEHGVGLEKKPYLGISRSEQEIELMRTIKRALDPKGILN
                     PGKIFDSASVNEAAA"
     gene            2403..2999
                     /locus_tag="AC16VR_01030"
     CDS             2403..2999
                     /locus_tag="AC16VR_01030"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6009762.1"
                     /translation="MARTFNKAYYDRYYRDPRTRVTTPAGTRRQAGFIAAYLDYLELR
                     PRSIVDLGCGTGTLLRALGKHFPRARLTGVEVSDYLCRRHGWLQGSVADFQAEHPFDL
                     VVCNDVLAYLDDAGCNRALRNLASLTGAALFLGVLTEEDLKLCDRRRTDPSQHARPAA
                     WYRRRLNRHWANVGGGLYLKRPLGVTVWTLDALNTVPS"
BASE COUNT          527 a         1041 c          969 g          557 t
ORIGIN      
        1 cgagctcccg aaagatctcc agggcttccc ggatcgtctc caccacgggc tcgtcgacgc
       61 ctttggacac gtagtcccag tcgacgccaa ttcgaaggcc cggcagggta tcggccacag
      121 aggcagcgta gttgggcact gggtcgggct gcgtggtgac gtcgtacgga tcggcgcccg
      181 cgagcacctg aagcagccgg gcggcgtcct caacgctgcg cgccatgggg cccacgtgat
      241 ccagcgactc ggctagggga aacgcaccgt atcggcttac ccttccgtac gtaggcttga
      301 tgccgacgag cccgcagcag gcgctcggaa aacggatgct gccgcccgtg tccgagccga
      361 gcgcgccaaa ggacagccct gccgccaccg ccaccccgga tcccgaagac gatacgccgg
      421 gccagtagtc gccgtcccag gggttcttcg gcgggtccac gtccgggtga tgggtcccgt
      481 aggcaccctc cgtgagctgc gtcttgccga cgatcaccgc acctgcatcc ttgagccggc
      541 gcaccaccgt ggcatcctcg tccggaaccc gctcgcccat gacccgagtg ccgctggcgg
      601 ttaccacgcc gcgggtatcg aacaggtcct tcaccgcgac gggaacgccg tggagggcgc
      661 ccagcggctt gccgtccgca cgatcctgat cgaggcgggc agcaaccgcg cgggcctcgt
      721 cggccatgac cgttgcaaag ctgttcagct tcgagtcgag ggcttcgata cgggccagta
      781 tcgcctcggt gaccgccgac gcgctgagct cgccgctctt catccggcga cacacaccgg
      841 ccagcgacag gtagtgaagg tcggtcatgc agccgcttcg ttcaccgatg cgctgtcgaa
      901 gatcttgccg ggattgagga tgcccttggg atcgagggcc cgcttgatcg tcctcatcag
      961 ctcgatttcc tgctcgctgc gggaaatgcc gagatagggc ttcttctcga gaccaacgcc
     1021 atgctccgcg gagaccgagc cctgaatcgc agcaagcggc tcgtagacgc acgcctccac
     1081 tgccctgcga gtctcgggac ctccctcccc gacggagatg accaggtgca ggtttccgtc
     1141 tcccatgtga ccgaaggtga aattgttgac gtcctgataa cgcgcttcca ggcgggcatt
     1201 gacctccgcg aggtatgact ccatgtggga gatgcgcagg ctcacgtcga aagtgtagat
     1261 cggggcgtaa cgggccgtgt gctccacgct gtctcgcaac gcccacagcg ccaggcgctg
     1321 agcttccgac tgcgcgatga ccgcatccgt aatgagagat ttttcgtaag cctcggcgag
     1381 ggcggcttcc accgcagcgt catcgctgcc catggcctcg accagcgcgt agtagggata
     1441 atcccgggga atcggcgcct ggttctccgc gggcggcgtg gtgaccaggg tatagaagtt
     1501 gttccacatg acctcgaagg cggaaagcgc gcctcccagc gctgcgtcca tgtgcttgag
     1561 gaactgcgcc agcttggcga actcgtccag cgagacgagc agggtcgcac ggcaggcagg
     1621 ctgctcacgc agccgcaaca ctgcccgggt gaccaccccg aggcttcctt ccgtgccgat
     1681 gaaaagctgc ttgaggtcgt agccggcgtt gttcttgatc atctggttca tgctggagag
     1741 caccgtgccg tcagcgagca ccgcttccag gcccagaacc atgtcccgcg tcatgccgta
     1801 gcgaatgacc cgattccctc cggcgttggt agatatgttg ccgccgatgg tgcagctgcc
     1861 gcgcccgccc agatcgagcg gaaacatgag ctggtgctcc tcggcagctt cctgaagcac
     1921 ctgcagaatt accccgctct gaaccaccat ggttcgatct accggattga tttcctcgat
     1981 ccggttcatg cgttccagcg agagagcgac gtcgtccggg ccggtaatgg cgctttccac
     2041 cagcccggtc agcccgccat ggggaacgat gctctggccg tgggcattgc agatggccag
     2101 ggtgcggctg acttccgcag tggtcctggg ccggacgagc gctttggcct ggatcgtatc
     2161 cgagcgccag atgccagcct ggcggctgtg cacgtcttcc ccggtcaaaa cgcagtcttc
     2221 ccccaaagct tcgatgagtt cggctacgac gacgtcagtc atggcttgat gtcctggaat
     2281 gcggcggaag ccgagcttac ctcagaagcg aacgtcctgc ctcaaggcca cccatccccg
     2341 ctggccggga gggtgcagca ttgtttacct ttggttctcg cccgaccctc aacgagcacg
     2401 tcatggccag aaccttcaac aaagcctact acgaccgcta ctaccgcgac ccgcggaccc
     2461 gcgtgaccac cccggcgggc acccggcgcc aggccgggtt catcgccgct tatctcgact
     2521 atctggagct ccggccgcgc agcatcgtgg acctcggctg cggaacgggc accctgctgc
     2581 gcgcgctggg caagcacttt ccacgagcga ggctgacggg ggtggaggtg agcgactacc
     2641 tgtgccgtcg ccacgggtgg ctgcagggat cggtagcgga tttccaggcc gagcatcctt
     2701 tcgatctcgt cgtctgcaac gacgtcctcg cctatctcga cgatgcgggc tgcaaccggg
     2761 ccctgcgcaa cctcgcctcg ctgaccggtg ccgcgctctt tctcggcgtg ctcaccgagg
     2821 aggacctgaa actgtgtgat cgccgccgca cggacccgtc ccagcacgcg cggccggctg
     2881 cctggtaccg acgacggctg aacagacact gggcgaacgt gggcggcggg ctctatctca
     2941 agcgccccct cggcgtaacc gtctggaccc tcgatgcgct gaacaccgtc ccctcctaga
     3001 gacgtcgaag gttgtcgtcg ggattgcggt cgatcatctt gttgtaagga tcgatgccta
     3061 cccgctcggg gcgctcgtcc acgcgtaccg tgaa
//
LOCUS       DBNHQR010000044         1334 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_954456, whole genome shotgun sequence.
ACCESSION   DBNHQR010000044 DBNHQR010000000
VERSION     DBNHQR010000044.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1334)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_954456"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1334)
                     /locus_tag="AC16VR_01035"
     CDS             complement(<1..>1334)
                     /locus_tag="AC16VR_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR6009763.1"
                     /translation="PYLRRRQGLEPVHYSDEAVRKVLERTLGVPIFQEQVIELAMVAA
                     GFSGGEADQLRRAMAAWRRRGGLGHFEKKLIDGMLARGHDREFAERVFRQIQGFGEYG
                     FPESHAASFALLVYISAWLKRHEPAAFYCGLLNSLPMGFYSPSQLIQDARRHGIEVLP
                     ADVQTSAWDHRLVTEQPSGEPGQQAVTPYRPGTYDRQPALRLGLRLVKGLNEEAGLRI
                     EAAAPFGDEADLARRADLSAQNLRFLARAGALKTLLGHRSQAHWQVAGVRAPMALEDS
                     GAAGYEIAGGQKTTKPDSGIGGADHKATVHLPAPDVADDMFDDYRYLSLTLGPHPMRL
                     LRANPMLAGHKTAVDLETMRQGQFVKVAGIVTGRQRPGTATGVLFVTLEDETGNINVI
                     VWASTLERYRAAVLQGQLLRIKGVVEREKEVIHVVAGCIEDATHLLEELASE"
BASE COUNT          209 a          447 c          452 g          226 t
ORIGIN      
        1 ttccgaggcg agctcctcca ataggtgagt cgcatcttcg atacatcccg ccaccacatg
       61 aatcacctct ttctcccgct ccaccacgcc tttgatcctc agcagctgcc cctgcagcac
      121 ggcggcccga tagcgctcca gcgttgatgc ccagacgatg acgttgatgt tgccggtctc
      181 gtcctccagg gtgacgaaga gcacgccggt ggcagtgccc ggtcgctgcc gcccggtgac
      241 gatgccggcc accttgacga actgtccctg acgcatagtc tcgagatcca cggcggtttt
      301 atgtcctgcc agcatcgggt tggcccgcag caaccgcatg gggtgggggc ccagtgtaag
      361 gctcaggtag cggtagtcgt cgaacatgtc gtcagctacg tcgggggcgg gcaggtgaac
      421 ggttgccttg tgatcggctc cgccgatgcc ggagtctggt ttggttgttt tctgtccgcc
      481 tgcgatctcg taaccggcag ccccagaatc ctccagggcc atcggcgcac ggacaccggc
      541 cacctgccag tgggcctggg agcggtggcc cagaagggtc ttcagcgcgc ctgcccgggc
      601 cagaaaacgc aggttctgcg cgctcaagtc cgctcgccgg gccaggtcgg cctcatcgcc
      661 aaacggcgca gcggcttcga tgcgaaggcc tgcttcctcg ttcaaaccct taaccaggcg
      721 caggccgagc cgcagagccg gctgacggtc gtaggttcca gggcggtagg gcgtgacggc
      781 ctgctgaccc ggctcgccgc tcggctgctc cgtgaccagc cggtggtccc aggcgcttgt
      841 ctgaacgtcc gccggcagca cctcgatgcc gtggcggcgc gcgtcctgga tcagctggga
      901 gggtgagtag aagcccatgg gcaggctgtt gagcagcccg cagtagaagg cggcgggctc
      961 gtgacgcttg agccaggcgg aaatgtagac gagcagcgcg aagctcgcgg cgtgggactc
     1021 gggaaagccg tactcgccga acccctggat ctgccggaag acgcgctcgg cgaactcccg
     1081 gtcgtggccc cgggccagca tgccgtcgat gagcttcttc tcgaaatgtc cgagcccgcc
     1141 gcggcggcgc caggcggcca tggcccggcg cagctggtcc gcctcgccgc cgctgaagcc
     1201 ggccgccacc atggcgagct cgatgacctg ctcctggaag atgggcacac caagggtgcg
     1261 ctcgagcacc ttgcgcacgg cctcgtcgga gtagtgcacg ggctcgagcc cctggcgccg
     1321 gcgcaggtag gggt
//
LOCUS       DBNHQR010000045         2062 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_827800, whole genome shotgun sequence.
ACCESSION   DBNHQR010000045 DBNHQR010000000
VERSION     DBNHQR010000045.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_827800"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="AC16VR_01040"
     CDS             complement(<1..778)
                     /locus_tag="AC16VR_01040"
                     /inference="COORDINATES: protein motif:HMM:NF014790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6009764.1"
                     /translation="MEDSAMDIELSTEDVEFRAEVRAFLKDNAYEAGTDYNEWRNGWF
                     EKAAEKGGWDVPKWPEAFGGPGWSPTQHYIWEQETAVATLPWDLPFGIGMLAPILMSY
                     GNEEQQKRFLPDIRARKVNWCQGYSEPGAGSDLASLKTKAVLSEDGSHYTVNGTKIWT
                     TVAHIADWIFCLTRTDDSGKKQEGITFLLFPMKQPGVEVKPIITLGGAHSVNTVFLTD
                     VKVPVENRIGEEGKGWTYAKGLLQHERTGLAGISRSIVALE"
     gene            849..1451
                     /locus_tag="AC16VR_01045"
     CDS             849..1451
                     /locus_tag="AC16VR_01045"
                     /inference="COORDINATES: protein motif:HMM:NF036525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6009765.1"
                     /translation="MANERITRATTPARRASVLDAALACFSERGLEGTTVSDIQSAAG
                     CSVGSIYHHFGSKEGIAEELFILGVQRLNQRMLKRLRRCKTGEESVRAVVGEYVRWAT
                     RESAMARYLHSRDVSFSADARERLRDIHREYVEALFAWFAPFVETGEMRRLPVQVYIP
                     LISGPIQEYVRRWLSGQHTRSPQQVEALFADAAWNAVKAR"
     gene            1474..>2062
                     /locus_tag="AC16VR_01050"
     CDS             1474..>2062
                     /locus_tag="AC16VR_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6009766.1"
                     /translation="MSYETLLFEVADGVGTITINRPEAFNAMSPLCAKEFADVAVRCD
                     DDPDVRAVVLTGAGKAFCAGGDLGAFASAGSSAKSLLKVMAGDLHMGLSRLARGNAPV
                     IAAVNGTAAGAGFSLVMAADLAIAAHSAVFTMAYTNAGLSPDGSSTFYMPRKIGDRRT
                     RELMLTNRLLKAEEALAWGVVNQVVADDQVVAEAMK"
BASE COUNT          350 a          634 c          698 g          380 t
ORIGIN      
        1 tctccaaagc cacgatggag cgggaaatcc ccgcaagccc ggtacgctcg tgctgcagca
       61 gccccttcgc gtaagtccag cccttgccct cctcgccgat gcggttttcc acgggaacct
      121 tcacgtcggt gaggaacacc gtgttgacgc tgtgggcacc tccgagggtg atgatgggtt
      181 tgacctctac gccgggctgc ttcatgggga aaagcaggaa ggtgattccc tcctgcttct
      241 tgccggaatc gtcggtgcgg gtcaggcaga agatccagtc ggcgatgtgg gctacggtgg
      301 tccagatctt ggtgccgttg acggtgtagt ggctgccgtc ctctgacaga accgccttgg
      361 tcttgagcga ggctaggtcg gagccggccc cgggctccga gtagccctgg caccagttga
      421 ccttacgggc ccggatgtcg ggcagaaacc gcttctgctg ctcctcgttg ccgtaggaca
      481 tgagaatggg agccagcatg ccgatgccga agggcaggtc ccagggcagg gtggccaccg
      541 cggtttcctg ttcccagatg tagtgctggg tgggtgacca tcccggtccg ccgaaggcct
      601 ccggccactt gggcacgtcc cagccgcctt tctcggcggc tttctcgaac cagccgttgc
      661 gccactcgtt gtaatccgtg ccggcttcgt aggcgttgtc tttcaggaac gcgcggacct
      721 ccgcgcggaa ctcgacgtcc tccgtcgaga gctcgatgtc catggctgaa tcctccattc
      781 gagaagggcc ggatattaac gctgattaga atcttttact agaactgaat tctagttacc
      841 atgacgccat ggccaacgag agaatcaccc gagcgactac cccggcgcgg cgcgcgagcg
      901 tcctggatgc ggcgctcgcc tgcttttccg aacgcggtct cgaggggacc acggtcagcg
      961 acatccagag cgccgccggc tgcagtgtgg gcagcatcta tcatcatttc ggcagcaagg
     1021 aaggcatcgc ggaggagctg ttcatcctcg gcgtgcagcg tctcaaccag cgcatgctca
     1081 agcgcctgcg gcggtgcaag accggggagg aaagcgtacg cgcggtggtg ggcgagtatg
     1141 tccgctgggc cacgcgggag tctgcgatgg cccgctatct gcactcacgc gacgtcagct
     1201 tctcggcgga cgcgagggag cggctgcggg acatccaccg ggagtatgtg gaggccctgt
     1261 tcgcctggtt tgcgccgttc gtggaaaccg gcgagatgcg ccgcctgccg gtccaggttt
     1321 acatccccct catcagcggt cccatccagg agtacgtgcg acgctggctc tccgggcagc
     1381 acacccgctc gccgcagcag gtggaggcgc tgtttgcgga tgcggcctgg aatgcggtaa
     1441 aagctcgata accgcacggg ttaaggcgta gagatgagtt acgagacgtt gctgttcgaa
     1501 gtggccgacg gcgtgggaac gatcaccatc aaccggcccg aggcattcaa cgccatgagc
     1561 ccgctgtgcg cgaaggagtt tgctgacgtg gccgtgcgct gcgacgacga tcccgacgtg
     1621 cgcgcggtgg tgctcacggg agcgggcaaa gcgttctgcg ccggtgggga cctgggcgcc
     1681 ttcgccagcg ccgggtcgag cgcgaagagc ctgctcaagg tgatggccgg ggacctgcac
     1741 atggggctct cgcggcttgc ccggggcaac gcgcccgtca tcgcggccgt gaacggcacg
     1801 gcggcggggg cgggattcag cctggtcatg gccgccgatc tggccatcgc tgcacacagc
     1861 gcggtgttca ccatggccta caccaatgcc ggcctttctc ccgacggcag ctcgactttc
     1921 tacatgccgc ggaaaatcgg cgaccggcga acgcgagagc tcatgctcac caaccggctg
     1981 ctgaaagccg aggaggcctt ggcctggggg gtcgtcaacc aggttgtggc ggacgatcag
     2041 gtggttgccg aggccatgaa gc
//
LOCUS       DBNHQR010000046         3409 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1398003, whole genome shotgun sequence.
ACCESSION   DBNHQR010000046 DBNHQR010000000
VERSION     DBNHQR010000046.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1398003"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="AC16VR_01055"
     CDS             complement(<1..1115)
                     /locus_tag="AC16VR_01055"
                     /inference="COORDINATES: protein motif:HMM:NF016873.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HIR6009767.1"
                     /translation="MRSLKDLLVVIPHSGIRIPPEIPSSSLTEEFPALLANVDWHTDR
                     LYDFTDLLENQQIVFPYCSLLLEANRDPELLDESIPLRDVNGKAVYRPGCEPSLDLRR
                     HLSYKYLRGFHQKISASISAGAEFLLDGHSTVVARGVAANQIDLMNFQHTQRDGEPKL
                     YAPAIYAETYAEALQKRLPEVHVTVNASEYYQVHGHVCAAHSVNGIGRAGQLAPALIQ
                     ETSQGLYMHPDGTPDVLAVDHLRRVFAEALRTTLDTVRRFTAPAQIIDLHGQRQTFDF
                     DCGVKALQMVLAYYGVEEREDKLLKALAADPELGTPLARMIEFAESRGFEVKAGTGWS
                     LDDVKHYLDRNCPVIVLVQAWADGYLSLAEWRRNFDDG"
     gene            1378..2778
                     /locus_tag="AC16VR_01060"
     CDS             1378..2778
                     /locus_tag="AC16VR_01060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HIR6009768.1"
                     /translation="MKKSPPPVEEEPPGGIHGQNLALSLHPPHSNTRSNKPITSRTAT
                     ADDARVIKVIPAKSTDSTSVASKAVESVQRRAKVVRLRTAGRGNPRTREFRIRHFPDV
                     SDAEWNDWRWQSRHRFKNLAQIERVLELSDDERAALTVGGMMLPVGITPYYMSLLDPV
                     DPQHALRRTVIPSQNEFLRLPGEADDPLGEDAHSPVPGLVHRYPDRVLMLPIDFCSTY
                     CRYCTRSRVVGHGELSPQTSRLEAMFDYLRGATQVRDVLVSGGDALALGEDNLDYILS
                     NLRAIEHIEFVRIGTKMPAVLPQRITRDLTRVLRKYHPLWMSLHFLHPEECTPESTQA
                     CERLADAGIPLGSQTVLLKGVNDDVGVMTRLVHGLLKMRVRPYYLYQCDPISGSAHFR
                     TTVEKGLEIIEGLRGHTTGYAVPTYVIDAPGGGGKIPIQPNYVLGRDVDELKLRNFEG
                     RVFSYPDPQQPGAGTN"
     gene            2781..>3409
                     /locus_tag="AC16VR_01065"
     CDS             2781..>3409
                     /locus_tag="AC16VR_01065"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF019121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR6009769.1"
                     /translation="MRIGITYDLRSAYLAAGYSEEETAEFDRDDTVDAIEAALRQLGH
                     ETVRIGCARELIEHLAAGQRWDLVFNICEGLSGPGRESQVPAILEVYGIPVTFADTAV
                     MALSLNKAWAKTVVADAGVATPQHAVIARAADVATVALEPPLFVKPIGEGTGKGTSAA
                     SVVMSAAELERQSLALLERYRQPVLVETYLPGREFTVGLLGTGPNARVLG"
BASE COUNT          614 a         1110 c         1083 g          602 t
ORIGIN      
        1 ccgtcgtcga agttgcgtcg ccattcggcc agcgagaggt agccgtcggc ccaggcttgg
       61 accaggacga tgaccgggca gttgcggtcg agatagtgct tcacgtcatc cagcgaccag
      121 cccgttccgg ccttgacctc gaagcccctg gactcagcga actcgatcat cctggccagc
      181 ggcgtgccga gttcggggtc ggcggcgagc gctttgagca gtttgtcttc gcgctcctcg
      241 acgccgtagt aggccagcac catctgcagc gctttcaccc cgcagtcgaa atcgaacgtc
      301 tggcgttggc catgcagatc gatgatctgc gccggcgctg tgaaccgtcg gacggtgtcc
      361 agcgttgtgc gcagggcctc ggcaaagacc cggcgcaggt gatctaccgc cagcacgtcc
      421 ggcgtcccgt cggggtgcat gtaaaggccc tgagaagttt cctgaatcag cgcgggcgcc
      481 agctgcccgg ctctgccgat accgttgacc gagtgggcgg cacagacgtg accgtgaacc
      541 tgatagtact ccgaggcgtt gacggtgacg tggacctcgg gcagccgttt ctgcagggcc
      601 tcggcgtatg tctcggcgta gatcgccggc gcgtacagct tcggctcgcc gtcgcgctgc
      661 gtatgctgaa agttcatgag atcgatctga tttgctgcca cgcccctggc gaccacggtg
      721 gagtggccgt ccaggagaaa ctcggcgccc gcggagatgc tcgcgctgat cttctgatga
      781 aagccgcgca ggtacttgta ggacaggtgg cggcgcaggt ccaggctcgg ctcacagccg
      841 ggccggtaga cggctttgcc gttgacgtcg cgcaggggta tcgactcgtc cagcaactcg
      901 gggtcgcgat tggcctcgag caggaggctg cagtacggga agacgatctg ctgattctcg
      961 agcaggtcgg taaagtcgta caggcggtcg gtgtgccagt ccacgttggc aagcagtgcc
     1021 ggaaactctt cggtgaggct cgaactcggt atctccggcg gaatgcgtat cccgctatgg
     1081 gggatgacca ccagaagatc tttcaggctg cgcatgcgcg acccttgaaa tgtctgaaga
     1141 acgtcataga aaacccggtc cgcagacatt gcgtgggacc ggcggggatc cgcagtaagc
     1201 gccaaaaatc cggcaaatac aatgggctcg cggcggcgtt tgcctacatg aaaaaaagtc
     1261 cggttgatgt gatgcacggc tattgcattc aaccccgttt gccttaactt acgcgccgtt
     1321 cggcagctcc gaggggctgt cgtttttcgt tggaccgcca caggagatcc aaggcgcatg
     1381 aaaaagtccc ccccaccggt cgaagaggag cctccgggag gtattcacgg ccagaatcta
     1441 gccctcagcc tgcatccacc ccattcgaat acccgatcca acaagccaat cacctcccgt
     1501 accgcgacag ccgacgacgc gcgtgtcatc aaagtcatcc ctgcaaagtc caccgactcg
     1561 acatccgttg catcgaaggc cgtcgagtcc gtgcagcgtc gggcgaaggt agtccggctg
     1621 cggaccgcag ggcgcggcaa cccccgcaca cgcgagttcc ggatccgcca tttcccggac
     1681 gtttccgatg cggagtggaa cgactggcgc tggcagtccc ggcaccgctt caagaatctc
     1741 gcccagatcg agcgcgtgct cgaactgtcc gacgatgagc gcgcagcgct caccgttggc
     1801 ggcatgatgc tgcccgtggg gatcacgccc tactacatga gtctgctcga ccccgttgat
     1861 ccgcagcatg ccctgcggcg gacggtcata ccctcgcaga acgaatttct tcgcctgccg
     1921 ggggaagccg acgacccgct cggggaggac gcccacagtc cggtgccggg gctcgtgcac
     1981 cgttaccccg accgggtgct gatgctgcca atcgatttct gttccaccta ctgccgctac
     2041 tgcactcgct cgcgggtagt aggccatggg gagctctccc cccagacatc gcgcctggaa
     2101 gccatgttcg attacctgcg aggcgcgacc caggttcgcg acgtgctcgt ctccggcggc
     2161 gacgcgcttg ccctcggcga ggacaacctc gactacatcc tttccaacct gcgggccata
     2221 gagcacatcg aattcgtccg catcggcacc aagatgccgg ccgtgctccc gcaacgaatc
     2281 acccgggatc tgaccagggt gcttcgcaag taccacccgc tctggatgag cctgcacttc
     2341 ctgcacccgg aggaatgcac gccggagtcc acccaggcct gcgagcggct ggccgatgcc
     2401 ggtataccgc tcggctccca gaccgttctg ctcaagggcg tgaacgacga cgtgggcgtg
     2461 atgacgcgcc tcgtgcacgg cctcctgaag atgcgggtgc ggccctacta cctgtaccag
     2521 tgcgacccga tcagtggttc ggcccacttc cgcacgaccg ttgagaaggg cctggagatc
     2581 atcgaagggc tgcgcggcca caccacgggt tacgccgtgc cgacctacgt catcgatgcg
     2641 cccggcggtg ggggcaagat tccgattcag ccgaactacg tgctcggcag agacgtcgac
     2701 gagctcaaac tccgcaattt cgagggacgg gtcttcagct atccggatcc ccagcaacct
     2761 ggcgccggga ccaactgaac atgcgcatcg gtatcaccta cgatctgcgt tccgcgtacc
     2821 tcgccgccgg ctactcggag gaggaaacgg cggagttcga ccgcgacgac accgtcgacg
     2881 ccatcgaggc ggcgttgcgc cagctcgggc acgagacggt gcgcatcggc tgcgcccgcg
     2941 agctcatcga gcaccttgcc gcgggacagc gttgggatct ggtgttcaac atctgcgagg
     3001 ggctcagcgg tcccgggcgg gagagccagg tgccagccat tctggaggtc tacggcatac
     3061 ccgtgacgtt tgccgataca gccgtcatgg ctctgagcct gaacaaggcc tgggccaaga
     3121 cggtggtggc cgacgccggt gtggcgaccc cgcagcatgc cgtcatcgcc cgggccgctg
     3181 acgttgccac ggtcgcgctc gagccgcccc tgttcgtcaa gccgatcggg gagggcaccg
     3241 gcaagggcac aagcgccgct tcggtcgtca tgagcgccgc cgagcttgaa cggcagagcc
     3301 tggccctgct cgagcgctac cggcagcccg tgctggtcga gacgtacctc cctggcagag
     3361 agttcaccgt cggccttctg ggcactggcc cgaatgccag ggtgctcgg
//
LOCUS       DBNHQR010000047         1960 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_131088, whole genome shotgun sequence.
ACCESSION   DBNHQR010000047 DBNHQR010000000
VERSION     DBNHQR010000047.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_131088"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="AC16VR_01070"
     CDS             complement(<1..337)
                     /locus_tag="AC16VR_01070"
                     /inference="COORDINATES: protein motif:HMM:NF037004.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009770.1"
                     /translation="MLAGTGLCIVAAWLASIAAAENPQLALSTPTETADLLSPLDVHP
                     RISLTIVEQLRHNHYLEKELDDQQSSHIFENYLGMLDGAKVYFTAADVAAMERYRYKL
                     DDALVRGNLV"
     gene            771..1448
                     /gene="bamJ"
                     /locus_tag="AC16VR_01075"
     CDS             771..1448
                     /gene="bamJ"
                     /locus_tag="AC16VR_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BamJ family beta-barrel-assembly machinery
                     protein"
                     /protein_id="HIR6009771.1"
                     /translation="MGCQPAGDGTAADAQAAEESAELALDDQVAQASYGIGYTIASNL
                     DSQFAGSLNTEALMAGLQDSMEGRERRLSEAAIQTAIAALAQKQQEAVAARSADVLAA
                     GRAFLEENGRRDGVSTTASGLQYEIIATGDGPKPGASDTVSTHYHGTLIDGSVFDSSV
                     ERGQPASFAVNRVIPGWTEALQLMSVGSKWRLFIPPELAYGDRAAGSIPPNSTLIFEV
                     ELLAVDG"
     gene            1496..1765
                     /locus_tag="AC16VR_01080"
     CDS             1496..1765
                     /locus_tag="AC16VR_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009772.1"
                     /translation="MSGERRRPELGNTLTKRRMEPRRRGRLARTILLGTVAVAFSLFW
                     VARELELDTEELLGFLGASAVFVGLMVILGLVGAAAVLIFKRLGR"
     gene            complement(1781..>1960)
                     /gene="lepB"
                     /locus_tag="AC16VR_01085"
     CDS             complement(1781..>1960)
                     /gene="lepB"
                     /locus_tag="AC16VR_01085"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR6009773.1"
                     /translation="VPPGQYLVLGDNRDNSRDSRSIGLVARDRILGKATAVAFSLNYD
                     RYYAPRVDRFFADLP"
BASE COUNT          335 a          589 c          677 g          359 t
ORIGIN      
        1 gcacgaggtt gccccgcacc agagcatcgt ccagcttgta gcgatagcgc tccatcgccg
       61 ccacgtcggc ggcggtgaag tacaccttgg caccatccag catgccgaga tagttctcga
      121 agatgtggct cgactgctgg tcgtccagtt ccttttcgag gtagtggttg tgacgcagct
      181 gctcgacgat ggtgaggctg atccgcgggt gaacgtccag cggcgacaga aggtcggcgg
      241 tctctgtcgg ggtagacagc gccagctgcg gattctcagc cgctgcgatg gaggcgagcc
      301 aggccgcaac gatgcataag ccggtgcccg ccagcatgac cgagcgggac cggaagaggg
      361 ttctagagct tttgcgcata acgtgagtga tctgatggtg ccctgaatga aaatgtgggt
      421 cgcattggcg gcgtcgggtc caggccctgg ccttcgtatt gttcgtaact cagtcgccgg
      481 ggatctgcag attttcgccg gtccggtcgg caaactcgct tctgccaccc gtttcggtac
      541 aaactgtgcc gcaactgatc gctacttctg tagataccat ttctcctggg tggttccccc
      601 aaaccactcc agcgagacta tctaaatgcc cgcatgcgta ctgttgcggg aatgtaaccc
      661 ggtgtctggg tgtgggcaac cggggcaggg agcccaaatt gatggaatgc cgaacggaca
      721 tgagtacttg gtcaagaaat ttcgcatctt tgctggtggt gctgctggcg atggggtgcc
      781 agccggccgg ggacggcacc gcggcggatg cgcaggcggc agaggaatcc gcagagctgg
      841 cgctggacga tcaggtggcc caggccagct acgggatcgg gtacaccatc gcgagcaatc
      901 tggactccca gtttgcgggc tccctgaaca ccgaggccct gatggcgggc cttcaggaca
      961 gcatggaggg tcgggagcgc cggctttccg aggcggcgat ccagaccgcc atcgcggcgc
     1021 tggcacagaa gcagcaggag gcggtggccg ctcgctccgc ggatgtgctg gccgccggcc
     1081 gggcgtttct ggaggaaaac gggcgccggg acggcgtcag cacgacggcg tcgggactgc
     1141 agtacgagat catcgcgacc ggcgacggtc ccaagccggg cgcctcggac accgtgagca
     1201 cgcactatca tggcaccctg atcgacggca gcgtgttcga cagctccgtg gaacgcggcc
     1261 agccggcaag ctttgccgtc aaccgggtga ttccgggctg gacggaagcg ctccagctga
     1321 tgtcggtggg ttccaagtgg cgtctgttca ttccgcccga gctcgcatac ggagatcggg
     1381 cagcgggctc catccctccc aactcgacgc tgatcttcga ggtcgagctc ctggccgttg
     1441 acggttagca cgcacgtggc tcatcgcgag cgggtctgga cggcggccgc ttcccatgag
     1501 cggtgagcgc cgccgccctg agctcggcaa cacgctgacc aagcggcgca tggagccccg
     1561 caggcgcggg cgccttgccc ggaccatact gcttggcacg gtggcggtgg cgttctcgct
     1621 gttctgggtc gctcgggaac tggagctcga caccgaggag ctgctgggtt tcctgggtgc
     1681 cagcgccgtg ttcgtcgggt tgatggtgat actcgggctg gtgggcgcgg ccgccgtcct
     1741 gatcttcaag cgcctcggcc gctgacggcc cgctcacgga tcagggaaga tcggcaaaaa
     1801 agcggtcaac cctcggcgcg tagtaccgat cgtagttcag ggagaaggcg acggccgttg
     1861 ccttgccgag aatccgatcc cgggccacca gaccgatgct gcgcgagtcc cggctgttgt
     1921 ctcgattgtc gccgagcacc aggtattggc cgggaggcac
//
LOCUS       DBNHQR010000048         7345 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1208197, whole genome shotgun sequence.
ACCESSION   DBNHQR010000048 DBNHQR010000000
VERSION     DBNHQR010000048.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7345)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7345)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7345
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1208197"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="AC16VR_01090"
     CDS             complement(<1..639)
                     /locus_tag="AC16VR_01090"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6009774.1"
                     /translation="MPSTPCDLIIDADWILPIVPAGSVLQEASIAIADGRILKIGDRE
                     LIHGEHQPREVHNLPGHALLPGLINAHGHAAMTLLRGFAEDAPLENWLSDHIWPTEAR
                     LVDADFVRDGARLAMAEMLKTGTTCFSDMYFFPEVTAAEARAAGMRAQIAFPVIEFPN
                     AWSASSDEGFHKGLALHDDYRRDPLIRVAFGPHATYSVSEGDLERILVYSEEL"
     gene            775..3360
                     /gene="gyrA"
                     /locus_tag="AC16VR_01095"
     CDS             775..3360
                     /gene="gyrA"
                     /locus_tag="AC16VR_01095"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020299636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR6009775.1"
                     /translation="MSDLGSDQGREILPVNIEDELRQSYLDYAMSVIVGRALPDVRDG
                     LKPVHRRVLFTMSELNNTFNRPYMKSARVVGEVMGKYHPHGDVAIYDTVVRLAQDFSM
                     RYMLVDGQGNFGSIDGDPPAAMRYTEVRMARIAGELLADLDKETVSFVPNYDDTLTMP
                     EVLPTRVPNLLVNGSSGIAVGMATNIPPHNLREVVAACLALLDDPSLTVDDLMVHIHG
                     PDFPTAAIINGRAGIVQAYRTGRGRIYVRARAEVISDGKSNKDTILITEIPYQLNKAR
                     LIEKIAELVKEKKIEGITELRDESDKDGLRVVIELRRGEPGEVVLNNLYAQTQLQSVF
                     GINCVALVDGQPRVLNLKQMLGAFVQHRREVVTRRSIYLLRRARQRGHILEGQAVALA
                     NIDAVVELIKASRTGADARTALMSRGWMSEAVQKLLERAGSDACRPEGLGEEFGFREG
                     LYYLSEEQAQAILELRLQRLTALEQDKLIEDYQVILDEIADLQDILGSEERLVSVIRA
                     ELEEIRDTYGDERRTEILSSHEDLSAEDLITEEDLVVTISHQGYAKTQSLDTYQAQRR
                     GGRGKAATAVKDEDFVEHLLIANSHATLLCFSNLGKVYWLKAYQIPRASRGAKGRPLV
                     NMLPLAADERITVVLPIEDFAEDRFVFMATANGTVKKTPLEQFARQRSTGLIALELEE
                     GNTLVGAAFTDGHCDVLLVSSSGKAVRFKESDVRAMGRTARGVRGIRLAGDHRVISLI
                     IPAPDGLILSASENGYGKRTVVDEFPVKGRGGQGVIAMQRSDRNGDVVGVVQVFGGDE
                     LMLISNMGTLVRTAADEVSVQGRNTQGVRLINLRGDEKLVGVERIVDSESAEADEESA
                     DADEE"
     gene            3357..4445
                     /gene="serC"
                     /locus_tag="AC16VR_01100"
     CDS             3357..4445
                     /gene="serC"
                     /locus_tag="AC16VR_01100"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HIR6009776.1"
                     /translation="MNRAINFSAGPAALPTQVLQQAQAELLDYAGTGMSVMEMSHRSA
                     EFIDIAERAERDFRELLQISDDYHVLFLQGGATLQFAMVPLNLLGDRGTADYVNTGSW
                     STKAIKEAARLCSVNVVASAEEHGFDRIPDPAAWACSADAAYLHICSNETIGGVQFHS
                     FPDRAGGPPLVADMSSDILSRPIDVSRFGLIYAGAQKNVGPAGLTLVVVRKDLVGAAR
                     PGTPSMLDYAVHAEAGSMSNTPPTYAWYLAGLVFAWIRSLGGVAALDELNRRKAGKLY
                     AAIDGSNLFRAPVVVEDRSIMNVPFTLPDGELDAAFLAGAKDRGLLNLKGHRSVGGMR
                     ASLYNAVPESAVDALISYMQEFETDHFR"
     gene            4442..5539
                     /gene="pheA"
                     /locus_tag="AC16VR_01105"
     CDS             4442..5539
                     /gene="pheA"
                     /locus_tag="AC16VR_01105"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HIR6009777.1"
                     /translation="MSTRDDRLKAVRDRIDAIDRELQKLLNERAACALEVGEIKQADP
                     DAEAPVFYRPEREAQILSRLKQENQGPLSDDDVARLFREIISCCLNLEQPLTIAYLGP
                     AGTYTEAATVKQFGHFAGTAALASIDEVFREVETGGAHYGVVPVENSTEGMVNHTLDC
                     FIDSPLKICAEVELPIHHAFMVAPDASGEGIEEILSHSQSLAQCRTWLDAHYPGVPRT
                     PVNSNAEAARLVAARTNAAAVAGDMAAERYGLRVIASHIEDQTDNKTRFLVIGQQRVG
                     PSGRDKTSILVSTRNEPGALYKILEPFHRHGISLSRIETRPARSGSWSYVFFIDFDGH
                     GSEPAIEAVMREVGDVAMEVRSLGSYPQAVV"
     gene            5540..6634
                     /gene="hisC"
                     /locus_tag="AC16VR_01110"
     CDS             5540..6634
                     /gene="hisC"
                     /locus_tag="AC16VR_01110"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 -
                     L-histidinol-phosphate:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HIR6009778.1"
                     /translation="MSPRVNPRIKALKPYQPGKPVEEVTRELGLVDVIKLASNENPRG
                     PGELVRQAIDAACGDLARYPDGNAFALKQALARHFGVRESQLTVGNGSNDVLDLAARM
                     SLSPGAEAVISEHAFVVYRLATLSAGGELVEVPARNYGADLDGILDAITDATAIVFLA
                     NPNNPTGTWVGEGALVRFLDEVPEHVWVVLDEAYHEYVEAAGYPNGMELQRRYPNLIV
                     TRTFSKIHGLAALRVGFSVSSEEAADLMNRARQPFNVNAPAMAAAVAALADQEFVQES
                     RRLNREGMGQMRDGIARLGLEQIPSVGNFLSFDVGGPCFPVYQALLEQGVIVRPIAEY
                     GLPNHLRVTVGLPEENARFLGALERVLGNS"
     gene            6639..>7345
                     /locus_tag="AC16VR_01115"
     CDS             6639..>7345
                     /locus_tag="AC16VR_01115"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HIR6009779.1"
                     /translation="MLLTVVGTGLIGGSFALGARTRNLFQTFVGIDPDPGNAQSALSL
                     GIVDEIADDVPAASDAVLIAVPSDAVAEWVVRLADHPGILFDAGSVKGAILDAIRGQL
                     GEIPERFVPCHPIAGREVSGPGAANEDLFAERLVVQTPVAGTVPEAQEQVAYWWRSLG
                     ARVESMDPELHDRVYALTSHLPHLIAFAYMQQIEPAHLDHAGGGFRDFSRIGGSDPDM
                     WSAIFGLNKDAVLAALAG"
BASE COUNT         1287 a         2355 c         2465 g         1238 t
ORIGIN      
        1 gagttcctcg gagtacacga ggatccgctc gaggtcgccc tcggagacgc tgtacgtggc
       61 gtggggcccg aacgcgaccc ggatgagagg atcgcgccgg tagtcgtcgt ggagcgccag
      121 gcccttgtgg aacccctcgt cggagcttgc gctccacgcg ttgggaaact cgatgacggg
      181 aaaggctatc tgggcccgca tgccggccgc ccgcgcctcc gcagccgtca cctcgggaaa
      241 gaagtacatg tccgagaagc aggtcgtgcc ggtcttgagc atttcggcca tggccagccg
      301 ggcgccgtcg cggacgaagt ccgcatccac gagccgagcc tcggtgggcc agatgtggtc
      361 gctcagccag ttctccagcg gcgcgtcctc cgcaaagccg cgcagcagcg tcatggcggc
      421 gtggccatgg gcattgatca gccccgggag cagcgcgtgg ccgggcaggt tgtgcacctc
      481 ccggggctgg tgctcgccgt gaatcagctc gcgatcgcca atcttcagga tcctgccgtc
      541 ggcaatggca atgctcgcct cctggagcac cgagcctgca ggcacgatgg gcagtatcca
      601 gtcggcatcg atgatcagat cgcatggggt ggacggcatc ggccgattat agcccctccg
      661 gccgggcgta ggcgggttca tagagcgcgg agacgggcgg aattccgtgg tcaaaagtat
      721 caggttcgcc tgcgcttcgt ggtaaaatgg cgcgcttttc tgcagcggtt ccatatgtcc
      781 gacctggggt ccgatcaggg ccgggagatc ctcccggtca acatcgaaga cgagcttcga
      841 cagtcttatc tcgattacgc catgagcgtg atcgtcgggc gcgcccttcc cgacgttcgt
      901 gacggcctca agcccgtgca caggcgggtg ctgttcacga tgagcgagct gaacaacacc
      961 ttcaaccggc cctacatgaa gtccgcccgg gtggtaggtg aggtcatggg caagtaccac
     1021 ccgcacggcg atgtcgccat ctacgacacc gtcgtccgac tggcccagga cttctccatg
     1081 cgttacatgc tcgtggacgg ccaggggaac ttcggctcca tcgacgggga cccgccggca
     1141 gccatgaggt acaccgaggt gcgcatggcg cgcatcgccg gggagctgct ggccgacctc
     1201 gacaaggaaa cggtgagttt cgtaccgaat tacgacgaca ccctcaccat gccggaggtg
     1261 ctcccgaccc gggttccgaa cctgctcgtc aacggcagct ccggtatcgc cgtgggcatg
     1321 gccacgaaca taccgcccca caatcttcgc gaggtcgtgg ccgcctgcct ggcgctgctg
     1381 gacgatccgt cgctgaccgt cgacgacctc atggtgcaca tccacggtcc ggactttccc
     1441 actgccgcca tcatcaacgg ccgggccggc atcgtgcagg cttaccgaac cggtcgcggg
     1501 cgcatctacg ttcgtgcgcg cgcggaagtg atcagtgacg gtaagtccaa caaagacacc
     1561 atattgatca ccgagatccc ctaccagctc aacaaggccc ggctcatcga gaagatcgcc
     1621 gagctggtca aggagaagaa gatcgaaggg atcacggagc tgcgggacga gtccgacaag
     1681 gacggcctgc gcgtcgtgat tgagctgcgc cgaggcgagc cgggcgaggt ggtgctgaac
     1741 aacctctacg cccagaccca gctccaatcg gtgttcggca tcaactgcgt ggccctggta
     1801 gacggtcagc ctcgggtgct gaacctaaag cagatgttgg gggcgttcgt tcagcaccgg
     1861 cgcgaagtgg tcacccggcg gtcgatctac ctgctgcgtc gagcccgcca gcgcggccac
     1921 atcctggaag gccaggccgt ggcgctggcc aacatcgacg ccgtggtgga gctcatcaag
     1981 gcctcccgga cgggtgcgga cgcgcgtacg gcgctcatga gcagaggctg gatgtcggag
     2041 gcggtccaga agctgctgga gagagccggc agcgatgcct gtcggccgga gggcctgggc
     2101 gaagagttcg gctttcgcga ggggctctac tacctctccg aggagcaggc ccaggcgatc
     2161 ctggagctca ggctgcagcg cctcaccgcc ctcgagcagg acaagctcat cgaagactat
     2221 caggtgattc tcgacgagat tgccgatctt caggacattc tcggctccga agagcggctc
     2281 gtctcggtca ttcgagcgga actcgaggag attcgggaca cctacggcga cgagcgcaga
     2341 acggagattc tctcctccca cgaggacctc tcggccgagg atctgatcac cgaggaagac
     2401 ctggtggtca ccatctccca tcagggctac gcgaaaaccc agtcgctgga cacttatcag
     2461 gcgcagcggc gcggcgggcg cggcaaggcg gccacggcgg tgaaggacga ggacttcgtg
     2521 gagcacctgc tcatcgccaa cagccacgcc acgctgctgt gcttttcgaa tctgggcaag
     2581 gtctactggc tgaaggccta tcagattccc cgcgcgagcc ggggcgccaa gggccggccg
     2641 ctggtgaaca tgctgccgct ggcggcggat gagcggatca cggtggttct tcccatcgag
     2701 gatttcgcgg aggatcgctt cgttttcatg gccaccgcca acgggacggt gaagaagacg
     2761 ccgctggagc agtttgcccg ccagcgctcc accgggctca tcgcgttgga gctggaggaa
     2821 ggtaacaccc tcgttggcgc cgcgttcacg gacggtcact gcgacgtcct gctcgtttcg
     2881 agctccggga aagccgtgcg cttcaaggaa tcggacgttc gcgccatggg ccgcaccgcc
     2941 aggggcgttc gcgggatccg cctggcgggt gatcatcggg tgatctccct catcattccg
     3001 gccccggacg gattgatctt gagcgcgagc gagaacggct acggcaagcg cacggtggtg
     3061 gacgagtttc cggtgaaagg acgtggcggc cagggcgtca tcgccatgca gcgcagcgac
     3121 cgcaacggcg acgtggtggg tgtcgtccag gtcttcggcg gagacgagct gatgctcatc
     3181 agcaacatgg gcacgctggt gcgaactgcg gcggacgagg tgtccgtgca ggggcgcaat
     3241 acccaggggg ttcgcctgat caacctgcgc ggcgacgaga agctcgtggg agtggagcga
     3301 atcgtcgatt cggagagcgc tgaggcggac gaagaaagcg ccgacgcaga cgaagaatga
     3361 accgcgcgat caacttctcg gccggcccgg cggcgctgcc cacacaggtg ctgcagcagg
     3421 cccaggctga acttctggac tacgccggca cgggcatgtc ggtcatggag atgagccacc
     3481 gcagcgcgga gttcatcgac atcgcggagc gcgccgagcg ggatttcaga gaactcctgc
     3541 agatcagcga cgactatcac gtgctgtttc tgcagggagg cgccacgctg cagttcgcca
     3601 tggtgcccct gaacctgctg ggcgatcgtg gcacggcgga ttacgtcaac acgggctcct
     3661 ggtcgaccaa ggcgatcaag gaagccgccc gcctgtgctc ggtcaacgtg gttgcgagtg
     3721 ccgaggagca cggtttcgac cggattccgg atcctgctgc gtgggcctgc agcgctgatg
     3781 ccgcctacct ccatatatgc tcgaacgaga ccatcggagg ggttcagttc cacagctttc
     3841 ccgaccgcgc cggcggcccg ccgctggtgg cagacatgtc gtcggacatc ctgtcccggc
     3901 ccatcgacgt cagccgtttc ggtctcatct acgccggcgc ccagaagaac gtcgggccgg
     3961 cgggactgac gctcgtcgtc gtgcgcaagg atctggttgg ggccgcgcgg cccggcacgc
     4021 ccagcatgct ggactacgcc gttcacgccg aggcggggtc catgtcaaac accccgccga
     4081 cctacgcctg gtacctggcg gggctggtgt tcgcctggat caggtccctg ggcggggtcg
     4141 ccgccctcga tgagctcaac cgccgcaagg cgggcaagct ctacgcggcc atcgacggca
     4201 gcaatctgtt ccgcgcgccg gtggtggttg aagaccgatc catcatgaac gtgccgttca
     4261 cgctccccga cggcgagctg gatgctgcat tcctggccgg cgccaaggac cgcggcctgc
     4321 tcaatctgaa gggacaccga agcgtgggcg ggatgcgggc gagcctttac aacgcggtgc
     4381 ccgaatccgc ggtggatgcg ctgatctcgt acatgcagga attcgagacc gatcacttca
     4441 gatgagcacc cgggacgaca ggctcaaagc ggtccgcgac cgtatcgacg ccatcgaccg
     4501 ggagctccag aaactcctga acgagcgggc tgcctgcgcg ctggaggtgg gtgagatcaa
     4561 gcaggcggac ccggatgcgg aggcgccggt gttctatcgg ccggagcggg aagcccagat
     4621 cctttcgcgg ctcaagcagg agaaccaggg gccgctgagc gacgacgacg tcgcgcgcct
     4681 tttccgcgag atcatttcct gctgtctgaa tctcgagcag cctctgacca tcgcttacct
     4741 cgggccggcg ggaacctaca ccgaagccgc caccgtcaag cagttcgggc actttgcggg
     4801 aacggcggcg ctcgcatcca tcgacgaagt gtttcgcgag gtggaaactg gcggcgccca
     4861 ttacggcgtg gtgccggtgg agaactccac ggaaggcatg gtgaaccaca cgctcgactg
     4921 cttcatcgac tcgccgctga aaatctgcgc ggaggtggag ctgcccatcc atcacgcctt
     4981 catggtggcg ccggacgcca gcggcgaggg catcgaggag attctgtccc actcccagtc
     5041 gctcgctcag tgccgcacct ggctggacgc gcactatccc ggcgttcccc gcacgcccgt
     5101 caacagcaat gccgaggcag cgaggctggt tgccgcccgt accaatgctg ccgccgttgc
     5161 gggcgacatg gcggccgaac gttacggact gcgggtcatc gcctcccaca tcgaggacca
     5221 gacggacaac aagacgcgct ttctggtcat cggccagcag cgcgtgggtc cgagcggtcg
     5281 tgacaagacc tccatcctgg tttccacccg caacgagccc ggcgcgctct acaagattct
     5341 cgagcccttc catcgacacg gcatcagcct gtcgcgtatc gagacgcggc cggccagatc
     5401 gggcagctgg tcctacgtct tcttcatcga tttcgacggg catggcagcg agccggcaat
     5461 cgaagccgtc atgcgggagg tcggcgacgt ggccatggag gtccgttcgc tgggctccta
     5521 tccccaggcg gtggtctgaa tgtcgccgcg cgttaacccc aggatcaagg cgctcaagcc
     5581 gtatcagccc ggcaagccgg tagaagaagt cacccgggaa ctcgggctgg tcgacgtgat
     5641 caagctggcg agcaacgaga accctcgcgg gcccggcgag ctggtgcggc aggccatcga
     5701 tgcggcctgt ggagaccttg cccgctaccc ggacggcaac gcgttcgcgc tcaagcaggc
     5761 gctggcccgg catttcgggg tgcgggagag ccagctcacc gttggcaacg gctccaacga
     5821 cgtcctcgat ctggccgcgc gcatgagcct gtccccgggg gccgaagcgg tgatctcgga
     5881 acacgccttc gtcgtctacc gcctggccac cctgtccgcg ggtggcgagc tcgtcgaggt
     5941 gccggctcgg aactacggcg cggatctcga cggaattctg gatgccatca ccgacgccac
     6001 ggccatcgtc tttctcgcca accccaacaa ccccaccggc acctgggtgg gcgaaggggc
     6061 gttggtgcgg ttcttggacg aggttcccga gcacgtctgg gtcgttctgg acgaggccta
     6121 tcacgaatac gtggaggcgg ccggataccc taacggcatg gagcttcagc ggcgctatcc
     6181 gaacctgatc gtcacgcgca cgttctcgaa gattcacggg cttgccgccc ttcgagtggg
     6241 cttcagcgtg agctccgagg aagcggccga cctgatgaac cgggcccgcc aacccttcaa
     6301 cgtcaacgcg ccggccatgg ctgctgccgt tgcggcgctc gcggatcagg agttcgtgca
     6361 ggagagccga aggctcaacc gcgaggggat ggggcagatg cgcgacggca ttgcccggct
     6421 tggcctcgag cagattccgt ccgtgggcaa tttcctcagc ttcgacgtgg gcggcccctg
     6481 ttttcccgtc taccaggcgc tgctcgagca gggcgtcatc gtacggccca tcgccgagta
     6541 cggccttccc aaccatttgc gcgtgaccgt cgggctgccc gaggagaacg ctcgctttct
     6601 gggggcgctg gaacgcgttc tcgggaattc ttaggccgat gctgctcacc gtcgttggga
     6661 ccggactcat cggcggctcg ttcgccctcg gcgcgcgcac ccgtaatctt ttccagacct
     6721 tcgtcgggat cgatccggac ccgggcaacg cgcagtctgc cttgagcctc ggcatcgtgg
     6781 acgagatcgc cgacgacgtc cctgccgcga gcgatgcggt gctgatcgcc gtgccgagcg
     6841 acgcggtggc cgagtgggtg gtgcggctgg ccgatcaccc cggcattctg ttcgatgccg
     6901 gcagcgtcaa gggggccatc ctggatgcaa tcagggggca gctgggcgag attcccgaac
     6961 gcttcgtgcc ctgccatccc attgccggcc gggaggtcag cggaccgggc gcggccaacg
     7021 aggacctctt cgcggagcgc ctggtggtgc agacgccggt ggcaggcacg gtcccggagg
     7081 cgcaggagca ggtggcctac tggtggcgca gcctgggcgc tcgcgtggaa agcatggacc
     7141 ctgaactgca cgatcgcgtg tacgcactga ccagccacct gccgcatctc atcgcgttcg
     7201 cctacatgca gcagatcgag cctgcgcatc tggaccacgc tggtgggggc tttcgcgact
     7261 tcagccgcat cggcggttcg gacccggaca tgtggtcggc gatcttcggg ctgaacaagg
     7321 acgcggtcct tgctgcgctg gcggg
//
LOCUS       DBNHQR010000049         1419 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_67618, whole genome shotgun sequence.
ACCESSION   DBNHQR010000049 DBNHQR010000000
VERSION     DBNHQR010000049.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1419)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_67618"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..256
                     /locus_tag="AC16VR_01120"
     CDS             131..256
                     /locus_tag="AC16VR_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009780.1"
                     /translation="MDWNINGASTAEWQRGLFGRLPHGRIDMQDTSYDLLGDFRG"
     gene            249..953
                     /locus_tag="AC16VR_01125"
     CDS             249..953
                     /locus_tag="AC16VR_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009401404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6009781.1"
                     /translation="MAEQKVCVVTGVGPGTGAAMVRRFHEGGCRVAMIARNAERLEAL
                     AGEMPNAHAYPCDMVDPARVEETLARIAGELAAPSVAIHNAVGGAFGDFLSIEPAQLE
                     ANFQVNVMGLLHLGRAVAPAMIEAGEGAIIVTGNTSALRGKAGFAGFAPTKAAQRILA
                     ESMARSLGPQHVHVAYIVIDAVIDLAWTRRRFADQPDGFFIQPASIAENAWYLAHQDP
                     SAWTFLSELRPHGENW"
     gene            complement(969..>1419)
                     /locus_tag="AC16VR_01130"
     CDS             complement(969..>1419)
                     /locus_tag="AC16VR_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009782.1"
                     /translation="AANRQLYGGDGYEASRIDAFLDANLVFAREAQVYLLGMDEPQAH
                     VHERMAAAYQFYLAGVESALERSAFLAGDELSIADISFVCDFAQFLREGHYEDALEKA
                     GLSLISGNGPEDYPRSFHHLLTLSAEPAFAQHMETYLDWYRRRAASA"
BASE COUNT          226 a          465 c          489 g          239 t
ORIGIN      
        1 tctcgggcaa cgcctgggag ttcgccgagt tcatcggccc attcgatgcc gagctcggcg
       61 tcaggcggca gacccatcgg gtggacaacg tgctgatcga gttcacgggg ccggacaccg
      121 cctctacgtg atggactgga acatcaacgg tgcgtccacc gccgaatggc agcgcgggct
      181 cttcggccgg ctgcctcacg ggcgcatcga catgcaggac acgtcctacg atctgctggg
      241 agatttccgt ggctgagcag aaggtctgcg tcgtcaccgg cgtggggccg ggtaccggcg
      301 ccgccatggt gcggcgattt cacgagggcg gctgccgggt ggccatgatc gcccggaacg
      361 ccgagcgcct cgaggccctg gccggcgaga tgccgaacgc gcacgcctat ccctgcgaca
      421 tggtggaccc ggcgcgggtc gaggaaacgc tggcgcgcat cgccggggag ctggcggcgc
      481 cttcggtcgc cattcacaac gccgtgggcg gcgctttcgg cgacttcctg agcatcgagc
      541 cggctcagct cgaggccaac ttccaggtga acgtcatggg tctgcttcac ctcggccggg
      601 cggtggcgcc cgccatgatc gaggcggggg aaggggccat catcgtcacg ggcaacacgt
      661 cggctttgcg gggcaaggcg ggcttcgccg ggtttgcccc caccaaggcg gcccagcgga
      721 tcctcgcgga gtccatggcc cgctcacttg gtccccagca cgtgcacgtg gcctacatcg
      781 tcatcgacgc ggtgatcgac ctcgcctgga cccggcggcg ctttgccgac cagcccgacg
      841 gcttcttcat tcagcctgca tccatcgcgg agaacgcctg gtacctggcg caccaggacc
      901 cgtcggcgtg gacgttcctg tcagagctca ggccacatgg cgagaactgg tagcggcgcg
      961 aacggccctt acgcgctcgc agcgcgcctt cgataccagt ccagataggt ttccatgtgc
     1021 tgcgcgaacg ccggctcggc gctgagcgtg agcaggtggt ggaagctgcg gggatagtct
     1081 tccgggccgt tgccgctgat caacgaaagg ccggcttttt ccagagcatc ctcgtagtgc
     1141 ccttctctca agaactgggc gaagtcgcag acgaaggaga tgtcggcaat gctcagctcg
     1201 tccccggcca ggaaggcgct gcgctccagc gcgctctcca cgccggcgag atagaactgg
     1261 taagcggccg ccatgcgctc gtggacgtga gcctggggct cgtccatgcc cagcaggtag
     1321 acctgggctt cccgggcgaa gaccaggttg gcgtctaaga aggcgtcgat gcgtgacgct
     1381 tcgtagccgt cgccaccgta cagctggcgg ttggccgca
//
LOCUS       DBNHQR010000050         7928 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_511221, whole genome shotgun sequence.
ACCESSION   DBNHQR010000050 DBNHQR010000000
VERSION     DBNHQR010000050.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7928)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7928)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7928
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_511221"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..2736)
                     /gene="polA"
                     /locus_tag="AC16VR_01135"
     CDS             complement(10..2736)
                     /gene="polA"
                     /locus_tag="AC16VR_01135"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HIR6009783.1"
                     /translation="MASDETAGAVSPPLLLVDGSSYLFRAYHALPELKTSTGLHTGAV
                     RGVIGMLRRLAKDYRESPIAVVFDAKGKTFRDDIYVQYKANRPPMPDDLSEQIEPIHQ
                     IIQAMGLPLIVVPGVEADDVIGTLAAEATRKRRDTVISTSDKDMAQLVTEHVTLVNTM
                     SETTLDPEGVVDKFGVPPELIIDYLALMGDSVDNIPGVPKVGPKTAAKWLNEYGDLDG
                     VVASAEQIKGKVGESLRDHLDQLPLSKALTTIKCDVALEVGIDDLAPRPVDEDRLKGL
                     FERLEFRSWLAEVGGPQEDAPAPAAASAEAEGEYECVSSAEALDEWIERLRAAPLFSF
                     DTETTSLNYMLAELVGVSFSCEPGKAAYVPLAHDYPGAPDQLGVAAALDRLKPLLEDP
                     SVIKVGQNLKYDMSVLQRYDVQLAGVGFDTMLESYVLNSTASRHNMDDLAKNYLSRET
                     IHYEDIAGKGAKQLTFNQVPVEQAVSYAGEDADITLQLHQTLWPRLEKEPRLTEVFRE
                     IEMPLLSVLSRMERKGTLVDGALLEAHGRELAERMRGITEEAWTLAGEEFNLDSPKQL
                     QAILFDKLELPVLKKTPKGQPSTAEPVLVDLARDFDLPRVILEYRSMAKLKSTYTDKL
                     PLQINQRTGRIHTSYHQAVAATGRLSSSDPNLQNIPVRTPEGRRIRQAFVAPPGKRIV
                     AADYSQIELRIMAHLSADDGLRAAFAANRDIHRATAGEVFGVGVDAVTDDQRRSAKAI
                     NFGLIYGMSAFGLARQLGISRTLAQEYIDRYFERYPGVLAYMDETRARARDAGYVETV
                     FGRRLYLPEINARNMQRRQAAERAAINAPMQGTAADIIKKAMLAVDAWLAGSELDAVM
                     IMQVHDELVFEVANADLDHLLAGVRDHMQGAADLDVELVVDCGTGANWDEAH"
     gene            2861..3121
                     /locus_tag="AC16VR_01140"
     CDS             2861..3121
                     /locus_tag="AC16VR_01140"
                     /inference="COORDINATES: protein motif:HMM:NF022639.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2782 domain-containing protein"
                     /protein_id="HIR6009784.1"
                     /translation="MAAAATLPPTALASEAQDGLQGPDITIIAQEDKTILEFRQAGQL
                     RMVKIVPRLGKPYYLVPRDSTTGFGDLERADMLLPSWVIVEF"
     gene            3152..4117
                     /locus_tag="AC16VR_01145"
     CDS             3152..4117
                     /locus_tag="AC16VR_01145"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HIR6009785.1"
                     /translation="MNAITEPGHPGIEETLTRYGVGELISAWPGSAASRCAYRVRTRR
                     AGREHDYLLLRVQAPRFEAELAALEACDRAGLPVAKAIPNLDGQPVDTLNGETVILTR
                     RLPGRHVYNPTVRQVAALGRFMARFHEAGGNLGPLPAWAWDQRWLAEASAALDGRLSY
                     PSARLMEATVQQVVSMLDREDVAGLPAGAIHGDICRDNVLFNEHGLSGVGGFSQVSSG
                     FLVYDLAVAANDWCIDANGMLDPERTLGFLRAYQKLRPLVRAELWFFSGFTLYAALNG
                     WIKRLAFAQGRQVSPGLHFNTPLELEKVVEQHAAHFFYLDERLLT"
     gene            complement(4240..4887)
                     /locus_tag="AC16VR_01150"
     CDS             complement(4240..4887)
                     /locus_tag="AC16VR_01150"
                     /inference="COORDINATES: protein motif:HMM:NF014667.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR6009786.1"
                     /translation="MRSVTATVLLCLSLAAAAVGAFVYSVLREPVLSDAELRDLGVFV
                     LPRPRAIGAFQLESTRGGSFTAADLQGGWSFLFFGFTNCPDVCPTTMAVLAQAERELL
                     AGSPEDADRFAGVLVSVDPERDDVRTLGEYTAAFSPRFFGVTGPFDELASFARQLNVG
                     FGKVPAPEGSFSEYLVDHGAQIVIINPQGHYHGFIRAPHTADTIVQTFASLAARF"
     gene            complement(4884..5795)
                     /gene="cyoE"
                     /locus_tag="AC16VR_01155"
     CDS             complement(4884..5795)
                     /gene="cyoE"
                     /locus_tag="AC16VR_01155"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016782738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HIR6009787.1"
                     /translation="MSEVSLQAASWSDYLEMTKPRVVLLMLLCALVGMFLAVPGMVPV
                     DVLVFGLVGIALVAGSAAVVNHIADAQIDARMARTQARPVATGRVGLLQGLLFSAVLG
                     VSGMVVLYFLVNPLTAWLNLASWIGYGLIYTLYLKRATPQNIVIGGLFGAAPPLFGWA
                     AVSNSVEPGALLLVLIIFAWTPPHFWALALDRKEEYASVDVPMLPVTHGERYTRLHIL
                     YYTLILVAVSLLPFVIGMSGWLYLAGATALGGGFIYWAVVLLRNRNPRAPLETFRYSI
                     VYLGLLFVALLADHYVFADHLVGSSLQ"
     gene            complement(5792..6724)
                     /locus_tag="AC16VR_01160"
     CDS             complement(5792..6724)
                     /locus_tag="AC16VR_01160"
                     /EC_number="1.17.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004423019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HIR6009788.1"
                     /translation="MLVVVLGAYTRLADAGLGCPDWPGCYGFLTVPESAEDIARAETL
                     FPDAPVERAKAWWEMVHRYFAAGLGLLVLVMAGRAYLRREQGGPVKLPLALLALVILQ
                     GAFGAWTVTMKLWPQVVTAHLLGGFTTLSLLWLLCLRLGVGSGWEVSGKLRTPALIAL
                     IVLVMQIALGGWVTSNYAALACPDFPTCHGEWLPEMDIGEGFNFAQSVGPNYLGGMLE
                     SEARVAIQLGHRLGALVATLAVGWLGLLLLRTGSPLGWPVLVLLAVQVVLGILNVLLA
                     LPLSNATLHNGFGAVLLLALVTVNYRAYLGGMRS"
     gene            complement(6768..7334)
                     /locus_tag="AC16VR_01165"
     CDS             complement(6768..7334)
                     /locus_tag="AC16VR_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009789.1"
                     /translation="MTQAEDKTGDQRGRAGRSQLVVILLVAGFSMAGAYLVFFLASSG
                     GVWGTTNNGEFVTPAMTMEALDLRDAAGTSATADGHWWLWTVAPGGCGEQCRFAVERL
                     RQVHALLNKDASRVRRALVTSTPGLEPPPELDVSDLERLVGPADGLASGIYIVDPIGN
                     LVLRYPLSDAGEAVLEDLKRLLKVSQIG"
     gene            complement(7331..>7928)
                     /locus_tag="AC16VR_01170"
     CDS             complement(7331..>7928)
                     /locus_tag="AC16VR_01170"
                     /inference="COORDINATES: protein motif:HMM:NF014193.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial respiratory chain
                     complex IV assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HIR6009790.1"
                     /translation="LASAPVAGRDTLAPEPFQRVRLEGRFEADRYFLVDNQVADGVVG
                     YWVVQSFSTPQGVRWLVNRGWIAGGDRRDQLPRVSAPTGTVVTVGVAWPDLGLVPLLD
                     EDDWPLAWPVRVQRLDVARMAERLEDAVPVEIRLETAQPGALRAAPLTVGLQAERHEG
                     YAAQWFALAGVLIVGYAVYGLRGAQRARRLNGRTDESR"
BASE COUNT         1449 a         2664 c         2540 g         1275 t
ORIGIN      
        1 cgccggagcc tagtgcgcct cgtcccagtt ggccccggta ccgcaatcca caaccagctc
       61 gacatccagg tcggcagcgc cctgcatgtg atcacggacg cccgccagca gatggtccag
      121 gtcagcattc gcaacctcga acaccagctc gtcatggacc tgcatgatca tcacggcatc
      181 cagctccgag cccgccagcc aggcatccac ggcgagcatc gccttcttga tgatatctgc
      241 cgcggtgccc tgcatgggcg cgttgatcgc ggcgcgctcc gcagcctgac gccgctgcat
      301 gttgcgggcg ttgatctccg ggagatagag ccggcgcccg aagacggttt ccacgtaccc
      361 cgcatcgcgt gcccgcgccc gggtctcgtc catgtaggcc aggacgccgg gataccgctc
      421 gaagtagcgg tcgatgtatt cctgggccag ggttcgggaa atgcccagct ggcgcgcgag
      481 gccgaaggcg gacatcccgt agataagccc gaagttgatg gccttggcgc tgcggcgctg
      541 atcatcggtg accgcatcga cgccgacgcc gaatacttca ccggccgtgg ctcggtggat
      601 gtcccggttc gccgcgaaag ccgcccgcag gccgtcgtcg gccgagagat gcgccatgat
      661 gcgcagctcg atctgagagt agtcggcggc tacgatcctt ttgcccggcg gcgccacgaa
      721 cgcctgccgg atgcgtcggc cttcgggcgt acgcaccgga atgttctgca ggttcggatc
      781 cgacgaggaa agccggcccg tggcggccac cgcctgatga tatgaggtgt gaatccgccc
      841 cgtgcgctga ttgatctgca gcggcagctt gtcggtatag gtcgatttca gcttggccat
      901 gctgcggtac tcgaggatga cgcgcggcag gtcgaagtcc cgggccaggt cgaccagaac
      961 gggctcggcg gtggagggct gacctttcgg cgtcttcttc agcacgggca gctcgagctt
     1021 gtcgaaaaga atcgcctgca gctgtttggg tgagtctagg ttgaattcct cgccggcgag
     1081 cgtccaggcc tcctccgtga tgccccgcat ccgctcggcc agctcgcggc cgtgggcttc
     1141 gaggagggcg ccgtccacca gcgtgccctt gcgttccatc cgggagagga cggacagcag
     1201 gggcatctcg atctccctga aaacctccgt cagacgcggc tccttctcga gcctgggcca
     1261 gagggtctgg tgcagctgca gcgtgatgtc cgcatcctcc ccggcgtacg acacggcctg
     1321 ttccacgggt acctgattga aggtcagctg cttggcgccc ttgccggcga tgtcctcgta
     1381 gtgaatggtc tcccgggaga gatagttctt tgccaggtcg tccatgttgt gacgggaagc
     1441 ggtgctgttg agcacgtagg actcgagcat ggtgtcgaaa cccactccgg cgagctgcac
     1501 gtcgtagcgt tgaagcacgc tcatgtcgta cttgaggttc tgccccacct tgatgaccga
     1561 cgggtcctcg agcagcggtt tgagccggtc aagggcggct gccacgccaa gctgatcggg
     1621 cgccccggga taatcatggg ccagcggtac gtaggcggcc ttgcccggct cgcagctgaa
     1681 cgacaccccc accagctcgg cgagcatgta gttaaggctg gtggtctcgg tgtcgaaaga
     1741 aaacagcggg gccgcgcgca gccgctcgat ccattcgtcc agcgcctccg cggagctgac
     1801 gcattcgtat tccccttcgg cttcggccga ggccgctgcc ggggcaggcg catcctcctg
     1861 gggacctccc acctcggcga gccagcttcg gaattccagc cgctcgaaca gccccttgag
     1921 gcgatcttcg tccacgggcc gaggggcgag atcgtcgatg ccgacttcca gagccacgtc
     1981 gcatttgatg gtggtgagcg ccttcgacag cggcagctga tcgagatgat cgcgcaggct
     2041 ctcgcccacc ttgcccttga tctgctcggc gctcgcaacg acgccgtcca ggtcaccata
     2101 ttcgttgagc cattttgccg ccgtcttggg gcccaccttc ggcaccccgg gaatgttgtc
     2161 cacgctgtcc cccatcagcg ccaggtagtc gatgatcaac tccggcggca cgccgaactt
     2221 gtcgaccacg ccctccgggt ccagggtggt ctcgctcatg gtgttgacca gggtcacgtg
     2281 ctcggtcacc agctgggcca tgtccttgtc gctggtcgaa atgacggtgt cgcgccgctt
     2341 cctggtggcc tccgcagcaa gggtgccgat cacgtcgtct gcctcgacgc ccgggaccac
     2401 gatcagcggc aggcccatgg cctggatgat ctgatgaatc ggctcgatct gctcactcag
     2461 atcatcgggc atgggcggcc ggttggcctt gtactggaca taaatgtcgt cccgaaacgt
     2521 tttgcccttg gcgtcgaaga ccacggcgat ggggctttcc cggtaatcct tggcgagccg
     2581 gcgcagcatg ccgatgacgc cgcgaacggc gccggtatgc aacccggtgc tcgttttcaa
     2641 ctccggcagc gcgtggtacg cccggaacag gtaggacgat ccgtccacga gaagaagcgg
     2701 cgggcttacc gccccggcgg tttcgtcgct ggccatgtgt cgaccttttt tatgatgcgg
     2761 atgctttgcc ttgcacagcc gtttcggtca acatccccct gtgcaacgct cgctccggac
     2821 actatcacag acgggccgca aaggcgcagc agtgctggcg atggcggctg ccgcgaccct
     2881 gccgcccacc gcgctggcaa gcgaagcgca ggacgggctg cagggcccgg acatcaccat
     2941 catcgcgcag gaggacaaaa ccatcctgga gtttcgccag gcgggacagc tcagaatggt
     3001 caagatcgtt ccccgcctgg gaaaacccta ctacctggtt ccaagagact cgacgacggg
     3061 ctttggcgat cttgagcgcg ccgacatgct gctgcccagc tgggtcatcg tcgagttctg
     3121 atacggccat gctggacagc tggaatagcc aatgaacgcc atcaccgagc ccggccaccc
     3181 gggaatcgaa gagaccctga cgcgctacgg ggtcggcgag ctcatctcgg cctggccagg
     3241 ctcagctgcc agccgttgcg cttatcgcgt cagaacccgg cgggccggaa gggagcacga
     3301 ctacctgtta ttgcgggtgc aggcgccccg attcgaggcc gagctcgccg ctttggaagc
     3361 ctgcgaccgg gcaggccttc cagtggcaaa ggcgatcccg aatctggatg ggcaacccgt
     3421 ggacacgttg aacggcgaaa cggtgatcct cacccggcgc ttgcccggcc gccacgttta
     3481 caaccctacc gtccggcagg tagcggcatt gggccggttc atggctcgtt ttcacgaagc
     3541 gggcggcaac ctcggaccgc tgccggcatg ggcctgggac cagaggtggc tcgccgaggc
     3601 gtcggccgct ctcgacggcc ggctctcgta tccgagcgcg aggctcatgg aagcaaccgt
     3661 ccagcaggta gtctccatgc tggaccggga agacgtggcg gggctgcctg ccggggccat
     3721 tcacggtgac atctgccgtg acaacgtgct gttcaacgag cacgggctga gcggcgtggg
     3781 cgggttcagc caggtgtcca gcgggttcct cgtctacgat ctggcggttg ccgccaacga
     3841 ctggtgcatt gacgccaacg gcatgctcga ccccgagaga acgctgggct tccttcgggc
     3901 gtatcagaaa ctgcgccccc tcgttcgagc cgagctctgg ttcttctcgg gttttaccct
     3961 gtacgcggct ttgaacggct ggatcaagcg cctggcgttc gctcagggcc gtcaggtgtc
     4021 cccagggctg cacttcaaca cgccattgga gctcgagaaa gtcgtcgagc agcacgccgc
     4081 gcactttttc tacctggacg aacggctgct cacctgaagc cgtttcgggc ttgccggctg
     4141 agcttaccgg ccgggcgtaa gagccggccg atcacaccgg ccccggactg acagagcggc
     4201 ggaaaccggc cgccagccgg cgacgcgggc aggccgcgct cagaaccgcg cggccagtga
     4261 cgcaaaggtc tgcacgatcg tatccgccgt gtggggcgcg cggatgaagc cgtgatagtg
     4321 gccctgcggg ttgatgatga cgatctgggc gccgtgatcg accagatact cgctgaaaga
     4381 cccttctgga gcgggaactt tgccgaatcc cacgttcagc tgccgggcaa acgaggcgag
     4441 ctcgtcgaaa ggtccggtca caccaaagaa gcggggggag aaagcggccg tgtactcacc
     4501 cagcgtcctc acgtcgtccc tttccgggtc aaccgaaacc agcaccccgg cgaagcggtc
     4561 ggcatcctcc ggtgagcctg caagcagctc ccgctcagcc tgggccagca cggccatcgt
     4621 ggttgggcac acgtcgggac aattggtgaa gccgaaaaag aggaacgacc aaccgccctg
     4681 aaggtcggcg gcggtgaagg aaccgccccg ggtgctctcc agctgaaacg cgccaatggc
     4741 gcgcgggcga ggcagcacga acacgccgag atcgcgaagc tccgcatcgc tgagtaccgg
     4801 ctcgcgcagg acgctgtaca cgaaggcgcc tacggcggct gcggccagcg acaggcacag
     4861 cagcaccgtc gctgtaacgg atctcactgc aggctcgatc ccaccaggtg gtcggcgaag
     4921 acgtagtgat cggcgagcag ggcgacgaag agcaggccga ggtagacgat ggagtagcgg
     4981 aaggtttcca gcggtgcccg cgggttgcgg ttgcgaagca ggacgacggc ccagtagatg
     5041 aacccgccac ccagggcagt ggccccagcc aggtatagcc agccgctcat gcctatcacg
     5101 aacggaagca ggctgacggc gaccagaatc agggtgtaat agaggatgtg cagcctcgtg
     5161 tagcgttccc cgtgggtgac cgggagcata ggcacgtcca ccgaggcgta ctcctccttg
     5221 cggtcaaggg ccagagccca gaaatggggc ggggtccagg cgaagatgat cagcaccagc
     5281 agcagggcgc ccggctccac gctgttgctc accgccgccc agccgaacag gggcggggcc
     5341 gccccgaaca ggccaccgat gacgatgttc tggggtgtgg cgcgcttgag ataaagggtg
     5401 tagatcaggc cgtagccgat ccaggaagcg aggttcagcc aggccgtgag cgggttgacg
     5461 aggaagtaga gaaccaccat tcccgacacg cccaggacgg ctgagaacag cagtccctgg
     5521 agcaaaccaa ccctgcccgt ggccaccggc ctcgcctggg tgcgcgccat tcgcgcgtcg
     5581 atctgagcgt cggcaatgtg gttgaccacc gccgcagagc cagcaaccag ggcaatgccg
     5641 accaggccga ataccagcac gtccacggga accatgcccg gcacggccag gaacatcccg
     5701 accagcgcgc agagcagcat cagcaggacg acccttggtt tggtcatttc aaggtagtcg
     5761 ctccagctgg cggcttgcag gctgacttcg ctcaacttct catccccccc aagtaggccc
     5821 gatagtttac tgtaaccaag gccagcagta ataccgcgcc gaaaccattg tgcagcgtgg
     5881 cgttgctcag gggtaatgcc agcaacacgt tgaggattcc cagcaccacc tgcacggcca
     5941 gaagaacgag aacgggccag ccgaggggcg agccggtgcg cagcagcagc aaacccagcc
     6001 agcccacggc cagggtggcg accaatgcac ccaggcggtg gccgagctgg atcgccacgc
     6061 gggcttcact ctccagcatc ccgcccagat agttcgggcc cacggactgg gcgaagttga
     6121 acccctcacc aatatccatt tccggcagcc actcgccgtg acaggtgggg aagtccggac
     6181 acgcaagggc ggcgtagttg gacgtcaccc aaccgccgag cgcgatctgc atcaccagca
     6241 cgatcagcgc gatcagggcc ggcgtcctga gctttccgga cacctcccag ccactgccga
     6301 caccgagccg caaacagagc agccacagga gactcagggt ggtgaagccg ccaagaaggt
     6361 gggcggtcac cacctgaggc caaagcttca tggtcacggt ccaggcaccg aaagcgccct
     6421 gcaggatgac cagcgccagc agcgccaacg gcagcttgac cggccccccc tgctccctac
     6481 gcaggtaagc gcgcccggcc atcaccagga ccagcagccc gaggccggcg gcgaagtaac
     6541 ggtgaaccat ctcccaccaa gccttggccc gctccaccgg cgcgtccgga aagagggtct
     6601 cggcccgggc gatgtcttcc gccgactcgg gcaccgtgag gaatccatag cagccgggcc
     6661 agtccgggca acccaggccc gcatccgcca accgcgtgta ggctcccagc acgacgacca
     6721 gcatcgccag cgccacgccg gccttcgaga gcgtcaggag ggtcttgtca gccaatttga
     6781 gacaccttca acagtcgttt cagatcctcc agcaccgctt cgccagcgtc ggacagcggg
     6841 taacggagca cgaggtttcc gataggatcc acgatgtaga taccggaagc cagcccgtcc
     6901 gccgggccga cgagcctctc gagatcggag acatccagct ccggcggcgg ctcgagcccc
     6961 ggcgtcgagg tgaccagcgc gcgcctgacc ctcgatgcat ccttgttgag cagcgcgtgc
     7021 acctgccgca gccgctccac cgcgaaccgg cactgctcgc cgcacccgcc gggcgcgacc
     7081 gtccacagcc accaatgccc gtctgccgtc gcggaagtgc ccgcggcgtc gcgcaggtcg
     7141 agggcctcca tggtcatcgc cggggtcacg aactcgccgt tgttcgtggt gccccagacg
     7201 ccaccgctgc tggcgagaaa gaagaccaga tacgcccccg ccatggaaaa gccggccacc
     7261 agcaggatca ccaccagctg gctgcgcccg gcgcggcctc tctgatcccc ggttttgtct
     7321 tcagcttggg tcatcgactc tcatccgttc gtccgttcaa tcgcctggcg cgctgcgcgc
     7381 cccgcagccc gtagaccgcg tacccgacga tcagcacgcc tgccagggca aaccactgcg
     7441 ccgcgtaacc ttcgtggcgc tccgcctgga ggccgaccgt cagcggcgcc gcacgcaggg
     7501 cgccgggctg ggccgtttcc agccggattt caaccggcac ggcatcctcc agccgttcag
     7561 ccattcttgc gacatcgagc cgctgcacgc ggacgggcca ggccaggggc cagtcatcct
     7621 cgtccagaag cgggaccagg cccaggtccg gccaggccac ccccaccgtg accaccgtgc
     7681 ccgtgggcgc cgacacccgg ggcagttggt cgcgacgatc gccgccggca atccagccgc
     7741 ggttgaccag ccagcgcacg ccctgcggag ttgaaaagct ctggaccacc cagtagccca
     7801 ccacgccgtc agccacctgg ttatccacca gaaaatagcg atcggcctcg aaccgccctt
     7861 ccagccgaac ccgctggaac ggctccgggg cgagggtatc acggcccgcc acgggagctg
     7921 aggcaagg
//
LOCUS       DBNHQR010000051         2543 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_4853, whole genome shotgun sequence.
ACCESSION   DBNHQR010000051 DBNHQR010000000
VERSION     DBNHQR010000051.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_4853"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="AC16VR_01175"
     CDS             complement(<1..710)
                     /locus_tag="AC16VR_01175"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6009791.1"
                     /translation="MLDLTIARAGPSAVRLLADWGADVIKIEPVPGAGQSVTGARRGP
                     DEQNLHRNKRSLCLDLKHPDGHGLFLELAAVADVVVENFKVDVKHRLRVDYETLKQVN
                     ESIIYASISGFGQEGPYADRGGVDQIVQGMSGLMSVTGEPGGGPMRVGVAISDTSAGM
                     FLGQGILLALLHRERTGEGQWVHTSLLEAMMCKLDFQGARYTMNGEVPTQQGNDHPAY
                     TPMGTFLASDGQVNIAAS"
     gene            complement(736..2433)
                     /locus_tag="AC16VR_01180"
     CDS             complement(736..2433)
                     /locus_tag="AC16VR_01180"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl-D-amino-acid deacylase family protein"
                     /protein_id="HIR6009792.1"
                     /translation="MSEHELVIRGGTVVDGTGSPERTADVAVRAGVIVAVGEVDGSGR
                     REIRADGALVTPGFVDIHTHYDGQATWSNRLSPSSHHGVTTVVMGNCGVGFAPVKPTD
                     RDLLIELMEGVEDIPGAALHEGIHWEWETFPQYLDYLSQRSFDMDIGAQLPHGALRVY
                     VMGRRGADREAASADDIALMRALTAEAIEAGALGFSSSRTLNHRSSKGAPTPSLTAAR
                     QELLGIARGLKDAGRGVMEMISDFQDADEEFAHLTAMTRESGRPMSISLAQGLSPNGW
                     RSLLGRIASASEAGLPMRAQVAPRPIGILLGLTTTLNPFVTHPAYREIAGLALAERVR
                     AMRDPERRAAILAGSPGRGFQRLWGLMANGAKIWRLEDPPDYEPAPQDSLASRARVAR
                     VDPWEYVYDQLLQAEGRQILYTPFANYAEDNLDCCREMILHPDTVMGLGDGGAHVGTI
                     CDASFVTTLLTHWGRDRTRGERIPRPVLVKSQTADTARAVDLNDRGVLAPGMKADINV
                     IDFERLKVRAPEIVHDLPAGGARLQQKADGYLATIVSGQVTYENGEATEALPGRLIR"
BASE COUNT          363 a          892 c          859 g          429 t
ORIGIN      
        1 atggaggcag cgatgttcac ctggccgtcg ctggccagga acgtgcccat gggggtgtag
       61 gccgggtggt cgttgccctg ctgggtcggg acctcgccgt tcatggtgta gcgcgcgccc
      121 tgaaaatcca gcttgcacat catggcttcg agcagcgacg tgtgaaccca ctgcccctcg
      181 cccgtccgct cccgatgcag cagcgccagc aggatgccct gccctagaaa catgccggcg
      241 gacgtgtcgc tgatggccac gcccacccgc atggggccgc ctcccggctc ccccgtcacc
      301 gacatgagcc cgctcatgcc ctggacgatc tgatccacgc cgccccggtc cgcgtaaggg
      361 ccctcctgac cgaagcccga gatgctcgcg tagatgatgc tttcgttaac ctgcttgagc
      421 gtctcgtagt ccaccctcag gcggtgcttg acgtccacct tgaagttctc gaccaccacg
      481 tcggccacgg ctgcgagctc gagaaacagc ccgtggccat ccgggtgctt gagatcgaga
      541 cagaggctgc gcttgttgcg gtgcaggttc tgctcgtccg gcccgcgcct ggccccggtc
      601 accgattgcc ctgcgcccgg cacaggctcg atcttgatca cgtcggcgcc ccagtccgcc
      661 agtagccgta cggcactcgg gcccgcgcgg gcgatggtga ggtcgagcac gcgaatgtgg
      721 gaaagcggcc ccatctcagc gaatcagccg gccgggcagc gcctccgtgg cctcgccgtt
      781 ctcgtaggtg acctggccgc tgacgatggt ggccagataa ccgtccgcct tctgctgcag
      841 gcgggcaccg ccggcgggga ggtcgtgaac gatctccggc gcgcgcacct tcaggcgctc
      901 gaagtcgatg acgttgatgt cggccttcat gccgggtgcg aggacgccgc ggtcgttgag
      961 gtccacggcc cgggccgtat ccgccgtctg gcttttcacc agcacgggtc gagggatgcg
     1021 ctcgccccgg gtgcggtccc ggccccagtg cgtgagcagg gttgtcacga agctggcatc
     1081 gcagatcgtg cccacgtggg cgccgccgtc acccaggccc atgacggtat ccgggtgcag
     1141 gatcatctcc cggcagcagt ccaggttgtc ctcggcgtag ttggcgaacg gcgtgtagag
     1201 aatctgccgc ccttcggctt gcagaagctg gtcgtagacg tactcccagg ggtccacccg
     1261 ggccacccgg gcgcggctcg ccaggctgtc ctgcggcgcc ggctcgtagt cgggcgggtc
     1321 ctctaaacgc cagatcttgg cgccgttggc catgagcccc cacaggcgct ggaatccgcg
     1381 ccccggggat cccgcgagga tggcggccct gcgttccggg tcgcgcatgg cgcgcacccg
     1441 ctcggccagc gccaggccgg cgatctcccg gtaggccggg tgggtgacga acgggttcag
     1501 cgtggtggtg agccccagga gaatgccgat gggccggggg gcgacctgcg cgcgcatggg
     1561 caggcctgct tcgctggcgc tggcaatgcg gccgagcagg ctgcgccagc cgttggggct
     1621 caagccctga gccagcgaga tggacatggg tcggccgctc tcccgcgtca tcgccgtcag
     1681 atgagcgaac tcctcgtcgg catcctggaa gtcggaaatc atctccatga cgccgcggcc
     1741 cgcgtccttg agcccgcggg cgatgccgag cagttcctgc ctggccgcgg tgagcgaagg
     1801 cgtcggtgcg cccttgctgc tgcggtggtt gagcgtgcgc gagctggaaa aacccagcgc
     1861 gccggcctcg atggcttccg ccgtgagcgc gcgcatcagg gcgatgtcgt ctgcgctggc
     1921 tgcctcccgg tccgcgcctc gccgacccat gacgtacacc cgcaaggcgc cgtggggcag
     1981 ctgtgcgccg atgtccatgt cgaacgagcg ctgcgataga tagtcgaggt actggggaaa
     2041 ggtctcccac tcccagtgga tgccttcgtg cagcgcagcg ccagggatgt cctccacgcc
     2101 ttccatgagc tcgatgagca ggtcccggtc ggtgggtttg acgggcgcga accccacgcc
     2161 gcagttgccc atcaccaccg tggtgacccc gtgatggctc gagggggaca ggcggttgga
     2221 ccaagtggcc tggccgtcgt agtgagtgtg aatgtccacg aagccgggcg tgaccagggc
     2281 gccgtcggcg cggatctcgc gccggcccga gccgtcgacc tcacccaccg ccacgatgac
     2341 cccggcacgc accgccacgt cggccgtccg ttccgggctg ccggtgccgt cgaccaccgt
     2401 gccgccgcga atcaccagct cgtgctcgct catctccacg tctcccggac atttcccccg
     2461 accgccggat tctcgcacat cgccccgtct tgtctacaat gcgcgctttc gcgactaaga
     2521 gcccgccgtt gcagcgcgtc acc
//
LOCUS       DBNHQR010000052         2340 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1651981, whole genome shotgun sequence.
ACCESSION   DBNHQR010000052 DBNHQR010000000
VERSION     DBNHQR010000052.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1651981"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..1131)
                     /gene="modC"
                     /locus_tag="AC16VR_01185"
     CDS             complement(25..1131)
                     /gene="modC"
                     /locus_tag="AC16VR_01185"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HIR6009793.1"
                     /translation="MSVDELDIEITASLPGFDLRLKRTLPLQGVTAVFGPSGSGKSTL
                     LRVLAGFIRPASGRVSCGGDVWFDKAANVHLPPHRRPVGMMFQDARLFPHLNVAGNLD
                     FAVRRQRRPGNGIEQPEVVAALDLGPLLTRQVDSLSGGERQRVALGRTLLASPRLLLL
                     DEPLAALDRERKADILPYLDQIQRGFGIPTLYVSHDVDEVAQLCDRVLVLAGGSERAF
                     GATADIVERLDLEPLMGRFEAGVLLEGTVTGHDPRLHVTHVDVGGDVLTLPLLSGVAE
                     GHAVRVRIRARDVALATTQPEGLSIRNVLPGRISRLLIEEASGTAEAFVELRGAHLRS
                     RLTLAAVEDLGLREGMPVYALIKSISFESSAPGG"
     gene            complement(1121..1792)
                     /gene="modB"
                     /locus_tag="AC16VR_01190"
     CDS             complement(1121..1792)
                     /gene="modB"
                     /locus_tag="AC16VR_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003113612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HIR6009794.1"
                     /translation="MPDLAPLWLTLHLATATTLILLVIGTPIAWWLARTRSPLRAPVE
                     ALTALPLVLPPTVLGFYLLVMLSPTSPFGSFWVQVTGEALTFSFAGLVVASVIYSLPF
                     MVQPLTNAFSAIGPGPMEAAASLGAGPFDAFRCVAAPLAKRGFVTAAALSFAHTVGEF
                     GVVLMVGGNIPGETKVISIAIYEHVETLRYAEAHLLSAGLVAFSFVVLLTVYGLNRRS
                     PLNVG"
     gene            complement(1793..>2340)
                     /gene="modA"
                     /locus_tag="AC16VR_01195"
     CDS             complement(1793..>2340)
                     /gene="modA"
                     /locus_tag="AC16VR_01195"
                     /inference="COORDINATES: protein motif:HMM:NF024921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR6009795.1"
                     /translation="QGAPFDLLLAADAWRPERLELEGRAVPGSRFTYAVGLLTLWSPR
                     PDAFGEDPVGYLRAGRLRRLALANPDLAPYGTAARQVLEHLGLQAQLADKMVFAENIG
                     QAHAMVDTGNADAGFLARAQVIGRPGSHWNVPVGMHLPIEQQAVLLRRAADNAAATGF
                     LGFLRTEAARGAMATAGYGLP"
BASE COUNT          397 a          778 c          837 g          328 t
ORIGIN      
        1 gctgaagagc tgcagagctg agtttcagcc gccgggcgcg ctgctctcga agctgatgct
       61 cttgatgagc gcatagacgg gcatgccttc ccgaagcccc agatcctcca cggccgccag
      121 cgtgagccgc gatcggagat gggctcccct gagctcgacg aaagcctcgg cggtgcccga
      181 cgcttcctcg atgagcagcc gggatatgcg acccggcagc acgttgcgga tgctcagacc
      241 ctctggctgg gtggtggcca gggcgacgtc ccgggcccgt atgcgcacgc ggaccgcgtg
      301 gccttccgcc acgccgctga gcagcggaag ggtgaggacg tcgccgccca cgtcgacgtg
      361 ggtgacgtgc aggcgagggt cgtgcccggt caccgtgccc tcgagcagaa cgccggcctc
      421 gaagcggccc atcagcggtt ccaggtcgag ccgctcgacg atgtcggccg ttgcgccgaa
      481 cgcccgctcg ctgccgccgg ccagaacgag cacgcggtcg cacagctgag ccacctcgtc
      541 gacgtcgtgg ctcacgtaga gcgtcgggat gccgaagccc cgctggatct gatcgaggta
      601 cgggaggatg tcggctttgc gttcgcggtc cagcgctgcc agcggctcgt cgagcagcag
      661 cagcctgggc gaggcgagca gggtgcgccc cagcgccacc cgctggcgct cgcccccgga
      721 gagcgaatcg acctggcgcg tgaggagggg acccagatcc agtgcggcca ccacctcggg
      781 ctgctcgatg ccgttgcctg gccgccgctg acgtcgcacg gcgaagtcca ggttcccggc
      841 cacgttcagg tgcgggaata gcctcgcgtc ctgaaacatc atgcccaccg ggcgccggtg
      901 tggcgggagg tgcacgttgg cggccttgtc gaaccagaca tccccgccgc aggagaccct
      961 gccggacgcg ggcctgatga agccggccag cactctgagc agggtgctct tgccgctgcc
     1021 gcttggtccg aacaccgcgg tgaccccctg tagcggcagc gtccgcttca ggcgcaggtc
     1081 gaaaccgggc agcgatgccg tgatctcgat gtccagctcg tcaaccgaca ttgagcgggg
     1141 acctccggtt tagaccgtaa acggtcagga gaacgacgaa gctgaaggcc accaacccgg
     1201 cggagagcag atgggcctcg gcgtaccgca gggtctccac gtgctcgtag atggcgatgg
     1261 agatcacctt ggtctcgccc gggatgttgc cgcccaccat caggaccacg ccgaactccc
     1321 cgacggtgtg ggcgaaagac aaggcggcgg ccgtgacgaa cccccgcttg gccagaggcg
     1381 cggcgacgca acggaacgcg tcgaacgggc cggcgcccag gctggcggcg gcctccattg
     1441 gccccgggcc gatggcggaa aaggcgttcg tcagcggctg gaccatgaag ggcagcgagt
     1501 agatgaccga tgccaccacg agtcctgcga acgagaaggt cagcgcctca ccggttacct
     1561 gtacccagaa gcttccgaac ggcgaggtcg ggctgagcat gacgagaagg taaaagccga
     1621 gcacggtagg tggcagcacc agtgggagcg cggtgagagc ttccacgggg gctcgcagcg
     1681 gcgagcgtgt gcgcgccagc caccaggcga tgggcgtgcc gatgaccagc aggatgagcg
     1741 tggtcgccgt cgccagatgc agagtcagcc acagtggcgc gaggtcgggc atctacggca
     1801 atccgtaccc ggccgtggcc atcgcgccgc gggctgcttc cgtgcgcaga aacccgagga
     1861 accccgttgc cgccgcattg tccgccgccc gccggagaag cacggcctgc tgctcgattg
     1921 gcaggtgcat gcccacgggc acgttccagt ggctgcccgg gcggccgatc acctgggcgc
     1981 gggcaagaaa accggcatcg gcgttgccgg tatcgaccat ggcgtgggcc tggccgatgt
     2041 tttccgcgaa caccatttta tcggccagct gagcctgcag gccgaggtgc tccagcacct
     2101 ggcgcgcggc ggttccgtag ggcgccagat ccgggttggc aagggcgagc cggcgcagac
     2161 ggccggcgcg cagatagcct accggatcct cgccgaaggc atccgggcgt gggctccaga
     2221 gggtgagcag acccacggca taggtgaatc gcgatccggg caccgcgcga ccttcgagct
     2281 ccaggcgctc gggtcgccag gcgtcggcgg cgagcagcag gtcgaagggt gctccctgca
//
LOCUS       DBNHQR010000053         2950 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_384819, whole genome shotgun sequence.
ACCESSION   DBNHQR010000053 DBNHQR010000000
VERSION     DBNHQR010000053.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_384819"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="AC16VR_01200"
     CDS             <1..788
                     /locus_tag="AC16VR_01200"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6009796.1"
                     /translation="RGDHVATTVCRGRSFCRKHATAEARTPEEAREHVRRAASLGVDF
                     IKTVHDRQVAPRVVASDELLAAIADESAALKIPLLIHVRDAADMVRLSALKPAAFVHA
                     PLTGELATAIEAAAMSLWTVPVSTTLAWQTPELFKARRARFHADDHERLEQALDNIRY
                     LVSTNSVVAFGTDNPPPLGTDDLMTEVRTLSRVLTPAEIIRSMTEHAAEFLGRGDELG
                     TLAPGKLADLVVVEGDPLTDLEALSRVRLVVKNGRVAADNRAR"
     gene            859..1887
                     /locus_tag="AC16VR_01205"
     CDS             859..1887
                     /locus_tag="AC16VR_01205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6009797.1"
                     /translation="MRHGIIFSGGGTSMQQMVDLACQAEQAGVDSIYLTEAWRSGFVG
                     LAAIAAATDRVEIGPYILNAYARSVWVTAMSAVDLDELSGGRLVLGVGSGNKHINEDW
                     QGIPQARPIRKMDEYVTLLRKAVSTRLGETLEWEGELHRMNWPPAVAPLRSTIPVYLA
                     ALYPQMIGVAGRVADGLALGALLSADYIGQELKPRFAKAAADADRDPDSLGIYFAPFV
                     SVGEEAEAARNAARAAICHLYSPLPHPYYDHVLREQGFAEAADACAAHVPEGRLEHAM
                     EAISDEMLDTVAVAGTAAQCRAALDQFEGIVDQALLVNVKYSGESEETLLAGFQDLIR
                     LAGIAGSY"
     gene            1900..2676
                     /locus_tag="AC16VR_01210"
     CDS             1900..2676
                     /locus_tag="AC16VR_01210"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6009798.1"
                     /translation="MELNAKRIIVTGCARGMGAATVRAYVGAGARVVGMDLAEEDGQK
                     VCIEASATGPGSAAYLKVDVADYESVEAMFAEAVGQLGGLDTLAHPAAIHAGSDAGDV
                     TVDDWDRMFAVNVRGTMITNQVARKHIKESGGGSIINFGSISGQRPEPTAAAYSAAKG
                     AVHAWTRTAAGTWGADNIRVNAILPAMATPMYYEARKRATEEENTASFWRNQQPIALG
                     QEYGDPDRDLGPVMVFLASDASRFITGQLIPVDGGQHNVR"
     gene            2740..>2950
                     /locus_tag="AC16VR_01215"
     CDS             2740..>2950
                     /locus_tag="AC16VR_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009799.1"
                     /translation="MAEPIPSREIDTWPEQALRGHRIPGSRYTSKEFAEKEWEGMWTK
                     VWLLLGREEELPNPGDWQCEEVGPES"
BASE COUNT          541 a          981 c          975 g          453 t
ORIGIN      
        1 ctcgcggcga ccacgtggcc accacggtat gccgaggccg ctcgttctgc cgcaagcacg
       61 ccaccgccga ggcgagaacg cccgaagagg cccgcgagca cgttcgccgc gctgcctctc
      121 tgggcgtaga cttcatcaag acggtgcacg accgtcaggt ggcgccccgc gtggtcgcgt
      181 cagacgagct gctcgccgcc atcgcagatg aatccgcggc gcttaagatt cccctgctca
      241 tccacgtccg cgatgctgcc gacatggtgc ggctgtctgc attgaaacca gcggcttttg
      301 ttcacgcgcc cctgaccgga gagctggcca cggccatcga ggcggcagcg atgagcctct
      361 ggacggtgcc cgtatccacc accctcgcct ggcagactcc agagctcttc aaggcgcgcc
      421 gcgcccgttt tcacgccgac gaccatgagc gtctggagca ggctctcgac aacatcagat
      481 atctggtcag cacgaactcc gtggtggcgt tcggaaccga taatcccccg cccctgggaa
      541 ccgatgacct catgaccgag gtgcgcacgc tgagccgcgt gctgaccccc gcggagatca
      601 tccgttccat gaccgagcac gcggcggagt ttctgggtcg cggcgacgag ctgggcacgc
      661 tggcgccggg gaagcttgcc gacctcgtgg tcgtcgaagg cgacccgctc accgatctcg
      721 aggcgctgtc gcgagtgcgt ctggtggtga agaatgggag ggtcgctgcg gataaccgcg
      781 cgcgctgacc ctacctaacc cttgggcaga actcggctcc tggtgtaagg tcccgtaagg
      841 gagtccaggg agagcaccat gcgtcacggc atcatttttt ccggcggcgg caccagcatg
      901 cagcagatgg tggatctcgc ctgccaggcg gagcaagcgg gggtggactc gatctacctc
      961 acggaagcct ggcgaagcgg cttcgtgggc ctggccgcca ttgcggccgc caccgaccgg
     1021 gtcgagatcg ggccgtacat tttgaacgcc tacgcccggt cggtctgggt caccgccatg
     1081 agcgccgtcg atctggacga gctcagcggc ggacggctcg tcctcggcgt cggcagcggc
     1141 aacaagcaca tcaacgagga ctggcagggt attccccagg cgcggcctat ccgcaagatg
     1201 gacgagtacg tcaccctgct gagaaaggcc gtgagcaccc ggctcggcga gacgctcgag
     1261 tgggagggcg agctgcaccg catgaactgg ccgccggccg tggcgccgct gcgcagcacc
     1321 atccccgtct acctggccgc cctgtacccg cagatgatcg gcgtggccgg gcgtgtggcc
     1381 gatgggctcg ccctgggcgc gctgctgtcc gccgactaca tcggccagga gctcaagcct
     1441 cgcttcgcca aggcggcagc tgacgccgac cgcgatccgg attccctggg catctacttt
     1501 gcgcccttcg tatccgtcgg cgaggaagcc gaggcggccc gcaacgctgc ccgggccgca
     1561 atctgtcacc tctactcgcc gctgcctcac ccttactacg accacgtgct gcgcgagcag
     1621 ggcttcgctg aggcggccga tgcctgcgcc gcccacgttc ccgaggggcg cctggagcac
     1681 gccatggaag ccatcagcga cgagatgctc gataccgtcg cggtagccgg cacggccgct
     1741 cagtgccgcg cggcgctgga tcagttcgag ggcatcgtcg accaggccct gctggtcaac
     1801 gtgaaatatt ccggcgagtc ggaagaaacg ctgctggcgg gctttcagga cctgattcgc
     1861 ctggcgggca tcgcaggcag ctactgaagc gaggaagtca tggaactgaa cgcgaagcga
     1921 atcatcgtaa ccggctgtgc cagaggtatg ggagcggcca ccgtcagggc ctatgtgggg
     1981 gccggcgcaa gggtcgtcgg catggacctc gctgaggagg atggccagaa agtgtgcatt
     2041 gaggcgagcg caaccggccc tggatcggcc gcttacctca aagtcgacgt agccgattac
     2101 gaaagcgttg aggccatgtt tgccgaagcc gtcgggcagc tcggcgggct ggacaccctg
     2161 gcccacccgg ccgccatcca tgccggatca gacgccggcg acgtcaccgt cgacgactgg
     2221 gatcgcatgt tcgccgtcaa cgtgcgcggc accatgatca ccaatcaggt agccaggaaa
     2281 cacatcaagg aaagcggcgg cggctcgatc atcaacttcg ggtcgatatc gggccagcgc
     2341 ccggagccca cggcggcagc ctactcggcg gccaaaggcg ccgtgcacgc ctggacgcgc
     2401 acggcggccg gcacctgggg cgcggacaac atccgggtaa acgcgattct tccggccatg
     2461 gccaccccca tgtactacga agcccgcaag cgagccaccg aggaggaaaa caccgcctcc
     2521 ttctggcgca accagcagcc aatcgcgctc ggccaggaat acggcgaccc ggaccgcgac
     2581 ctggggccgg taatggtgtt cctcgcctca gatgcctcgc gattcatcac aggccagctg
     2641 attcccgtgg acggcggcca gcacaacgtg cgctgacgcc cgttggcggg aaccgtccat
     2701 caggaatatt cttgaaagca agaaagcgga gctaggacca tggctgaacc gataccctcc
     2761 cgagaaattg acacctggcc cgagcaggcg ctgcgcgggc accgcatacc aggatcccgc
     2821 tatacctcga aggagtttgc cgagaaagag tgggaaggca tgtggaccaa ggtctggctg
     2881 ctgctgggcc gggaagagga gctgccgaac cccggcgact ggcagtgcga ggaggtgggc
     2941 cccgagtcca
//
LOCUS       DBNHQR010000054         2830 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1144642, whole genome shotgun sequence.
ACCESSION   DBNHQR010000054 DBNHQR010000000
VERSION     DBNHQR010000054.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1144642"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2830
                     /locus_tag="AC16VR_01220"
     CDS             <1..>2830
                     /locus_tag="AC16VR_01220"
                     /inference="COORDINATES: protein motif:HMM:NF015228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HIR6009800.1"
                     /translation="AYIEAVVARAEDPGRTAAPVVIRQLGRVVEEVSDGPFYAATVEM
                     DFDGEPRLVGFIAQDRSSRNGVWMPAHHLAAANRIQNYAKRSQPIVTMMDTPGADAEE
                     QANRENQAHSISRLIAEMSNVDVPNVGIIYGLGYSGGAIPLAASNMILSVRDGVFSTI
                     QPKSLANIARRLNLSWQECAKYVGLSPYELYAQGNIDGVIDYVPDDDVANLRLAIVSG
                     VQQVEEKVKEFVAENPYILDHYRQSLQRYLNPSEQLKVMQASASMKLTKNPTEYLNVF
                     GVAYRYLRYLKVRQRIKATSTHQYGRLAVRELPQGELTQRAELERRMLFLKWLQDPDR
                     VVYDDALSRAWKNYSDKKQAVHDERGRLAQFLFGEPKKNYEDARALLISSVGVFLYNR
                     WKSESLGNLRALKEMLTRHEDIRHILTVSDLPDARGLIKALRSDYALLPRLREGFTHE
                     GKKLIGEERLEDKSDLYLSSQLTAELNLVITGPLVAPEAGSPRSSDMARNRAYLVERY
                     PGLVMATAKEGAPVPFSDMTVLDVLVSDDLRRDFVTECENLLLFDSVYDQIIANLDSI
                     AEEAQSRQALAKTSLAQLIDTTLGLAATGVTLGGRGSGEPAETSETAKLRRQFIDWYL
                     RIARMPKGADFFRAVEEWKKAVFPHLSDTLFVVVTHLFDSLLLSFVQSEREGKAYNGR
                     IAPKNIGRRKDFWNRLNIAYRDLLIQNVLRDAKNRRTTGYKAFVDTFFSAFDERDADL
                     LSSDPCEFPGFRLSIEEALNRGIPPCGVVTGIGNFKTGDGDVRVGTVISNVDFQAGSF
                     DMASAEKFCRLLVECAEQHLPVVCFISSGGMQTKEGAGSLFSMAAVNDRITRFVRDHD
                     LPVIVFGFGDCTGGAQASFVTHPLVQTYYLSGTSMPFAGQIVVPSNLPLNSILSNYLS
                     VVAGAMQGLVQHPFLPELDKELRHIDPEI"
BASE COUNT          527 a          916 c          873 g          514 t
ORIGIN      
        1 gcctacatcg aggccgtggt cgcgcgagcg gaggatccgg gccgcaccgc ggcgcccgtg
       61 gtcattcggc agctcggacg ggtcgtcgag gaagtttccg acggtccctt ttacgctgcc
      121 acggtggaga tggatttcga cggcgagccg cgcttggtcg gcttcattgc ccaggatcgc
      181 tcctcccgca acggcgtttg gatgccggcg catcatctgg ccgctgcgaa ccgcatccag
      241 aactacgcca agcgctccca gcccatcgtc acgatgatgg acacgcccgg cgccgatgct
      301 gaggagcagg caaaccggga gaatcaggct cacagcattt cccggctcat cgccgagatg
      361 agcaacgtgg acgttcccaa cgtcggcatc atctatggtc tcggctactc gggcggcgcg
      421 ataccgctgg ccgccagcaa catgatcctg tcggtccgcg acggggtctt cagcaccatc
      481 cagccgaaaa gcctggccaa catcgcgcgt cggctgaacc tgtcctggca ggagtgcgcc
      541 aagtacgttg gcctgtcgcc ctacgagctc tatgcccagg gcaacatcga cggggtcatc
      601 gactatgtgc ccgacgacga cgtggccaac ctccgcctgg ccatcgtgtc cggggtgcag
      661 caggtcgaag agaaggtcaa ggagttcgtc gcggagaacc cctacatcct cgaccactac
      721 cggcagagcc tgcagcgcta cctgaaccct tcggagcagc tgaaggtcat gcaggccagc
      781 gcgtcgatga agctgacgaa gaaccccacg gagtatctca acgtcttcgg cgtggcgtac
      841 cgctatctgc gttacctcaa ggttcgacag cgtatcaagg ccaccagcac ccaccagtac
      901 gggcgtctgg cggtgaggga gctcccccag ggcgagctca cccagcgggc ggagctcgaa
      961 cggcgaatgc tgttcctcaa atggctgcag gatccggacc gggtcgtcta cgacgatgcc
     1021 ctttcccgtg cctggaagaa ctacagcgac aagaagcagg cggtgcacga cgagcgcggc
     1081 cggcttgctc aatttctctt cggtgagccc aagaagaact acgaggatgc ccgggcgctg
     1141 ttgatctcct ccgtgggcgt cttcctctac aaccgctgga agtccgagtc ccttggcaac
     1201 ctgcgcgctc tcaaggagat gctcacacgt cacgaggaca ttcgacacat cctaacggtc
     1261 agcgacctgc ccgacgcccg cggcctgatc aaggcgctgc ggtccgacta cgcgctgctg
     1321 ccgcgcctgc gtgaaggatt cacccacgaa ggcaagaagc tcatcggcga ggaacgcctc
     1381 gaggacaaat cggatctgta tttgagctca cagctcaccg ccgagctgaa cctggtcatc
     1441 accgggccgc tggtagcccc ggaggcaggg agcccgagat catcggacat ggcgcgcaac
     1501 cgcgcctacc tggtggagcg ctatccgggc ctggtgatgg caacggcaaa ggaaggtgcg
     1561 ccggtgccct tcagcgacat gaccgtgctg gacgtgctcg tctcggacga tctccggcgc
     1621 gacttcgtga cggagtgcga aaatctcctg ctgttcgact ccgtctatga ccagatcatc
     1681 gccaacctgg attccatagc cgaggaggcc cagtctcggc aggcgctggc caagacatcg
     1741 cttgcgcagc tcatcgacac caccctcggc cttgccgcta ccggagtcac cctggggggc
     1801 agaggcagtg gcgagccggc ggagacgtcc gagacggcga agcttcgccg gcagttcatc
     1861 gactggtatc tgcgcatcgc gcggatgccc aagggtgccg atttcttcag ggcggtcgag
     1921 gaatggaaga aggcggtctt tccgcacctc tctgacaccc tgttcgtggt ggtcacgcac
     1981 ctcttcgaca gcctgctgct ttccttcgtg cagtcggaac gcgagggcaa ggcttacaac
     2041 gggcggatcg cgccgaagaa catcgggcgg cgcaaggatt tctggaaccg tctgaacatt
     2101 gcgtaccgtg accttctcat ccagaacgtg ctgcgcgatg ccaagaaccg ccgcacgacg
     2161 ggttacaagg ccttcgtcga cacgttcttc tccgccttcg acgagagaga cgccgatctt
     2221 ctgagctcgg atccctgcga gtttccggga ttccggttgt ccatcgagga agccctgaat
     2281 cgcggcatcc cgccctgcgg cgtggttacc ggcatcggta acttcaagac cggcgacgga
     2341 gacgtccggg tgggaacggt gatttccaac gtggatttcc aggccggctc cttcgacatg
     2401 gccagtgccg agaaattctg ccggctgctc gtggagtgcg ccgagcagca cttgccggtg
     2461 gtctgcttca tctcctcggg cggcatgcag acgaaggagg gcgcgggctc gcttttctcc
     2521 atggccgccg tcaacgaccg catcacgaga ttcgtccgcg accacgacct gccggtcatc
     2581 gtgttcgggt tcggcgactg cacgggggga gcccaggcga gtttcgtcac gcatcccctg
     2641 gtgcagacct actacctttc agggaccagc atgccgttcg ccggccagat cgtggtgccg
     2701 tcgaacctgc cgctcaattc gatcctctcc aactatcttt ccgtggtcgc cggcgccatg
     2761 caggggctgg tccagcaccc gtttcttccg gagctcgata aggagctgcg gcacatcgat
     2821 cctgagatac
//
LOCUS       DBNHQR010000055         2501 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_5138, whole genome shotgun sequence.
ACCESSION   DBNHQR010000055 DBNHQR010000000
VERSION     DBNHQR010000055.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_5138"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="AC16VR_01225"
     CDS             <1..402
                     /locus_tag="AC16VR_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009801.1"
                     /translation="EMASVLLTRAIARLDKTAFSRLTKKQPAPVRLGRFVDAFSSYVD
                     HGERPCLIAVLDHGALRAVHGDTIRRRFLSWGERLAATFEETGMKPRRARRAASDLLA
                     GLYGNLAMARMLDDASLFQRGTRRLKKDLPS"
     gene            complement(399..1838)
                     /locus_tag="AC16VR_01230"
     CDS             complement(399..1838)
                     /locus_tag="AC16VR_01230"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase family protein"
                     /protein_id="HIR6009802.1"
                     /translation="MAKYDNNLIVIGAGSAGLIVALIAATVRARVTLIEQGAMGGDCL
                     NTGCVPSKTLIRSAKIAHYQATAQRYGVQAVTPQVDFQSVMRRVHEVIATIEPKDSMA
                     RYRELGVNCVAGAARLIDRHHVQVGDERLSARSIVLATGAEPVVPPIPGLADARPLTS
                     DNLWELESLPQRLLVMGGGPIGCELAQAFARLGSAVTLIDMAPGLMPREDDDVAGFVE
                     RRLRGEGIDVRTGHRAVRVVASADGAGVLHADSDGGEVQIGFDRVLVAVGRRANTAGL
                     GLEALGIATRSDGTLEVDAYLRTAVRNVYGCGDVVGPYQFTHMASHQAWYAAVNALFG
                     RFRKFKVDYSVVPWVTFTDPEVAHVGLSETEAAERGIRVEVTRYGLDDLDRALADGSA
                     SGWVKILTRPGSDRILGVSIVGAHAGELIAEYVLAMTHGIGLKKILGTIHVYPTLAEA
                     NKLAAGAWRRAHAPERLLRWVGRLHDLLR"
     gene            complement(1867..>2501)
                     /locus_tag="AC16VR_01235"
     CDS             complement(1867..>2501)
                     /locus_tag="AC16VR_01235"
                     /inference="COORDINATES: protein motif:HMM:NF020893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HIR6009803.1"
                     /translation="FFLTGLNDYLSLSFLKGVHHDAVVFVRENALVSILGFFLFYIAV
                     TALSLPGAAIMTLAAGAVFGLFWGTLITSFASSAGATLAMLISRTLLRDWVHARFAAQ
                     LRVVNDGLARDGAFYLFSVRMVPLFPFFIVNLVMGLTAIGTWQFYWVSQVGMLAGTLV
                     FVFAGTQLANVDTVADVLSPGLIAALSLLGLFPLIARKTMGWLERARAAR"
BASE COUNT          458 a          836 c          838 g          369 t
ORIGIN      
        1 gagatggcgt cggtgctcct gaccagggcg atcgcccggc tggacaaaac ggccttttcc
       61 cggctgacca agaagcagcc tgccccggtt cgtctcggcc ggttcgtgga cgctttcagc
      121 agctacgtgg atcacggcga gcgcccctgt ctgatcgcag tgctggacca cggcgcgttg
      181 cgggccgtgc acggtgacac catccgccgg cggtttctga gctggggcga acggcttgcc
      241 gccacgttcg aggaaaccgg catgaaaccg cgccgggcgc gtcgggcggc ttccgatctg
      301 ctggccggac tgtacgggaa cctggcaatg gccaggatgc tggacgatgc gtcgctgttc
      361 cagcgcggca ccaggcgact gaagaaggac cttccttcct agcgcaacag gtcgtggagc
      421 ctgccgaccc agcgtagcag ccgttcgggc gcgtgggcgc gccgccatgc ccccgcagcg
      481 agcttgttcg cctcggcgag ggtcgggtag acgtgaatgg tgccgagaat cttcttcagc
      541 ccgatgccat gggtcatggc cagcacgtac tcggcgatga gctcgcccgc atgggctccc
      601 acgatggaaa cgcccagaat gcgatccgag ccgggtctgg taaggatctt cacccaaccc
      661 gaggcgctgc cgtcggcgag cgcccgatcc agatcgtcca gaccgtaccg agtgacctcg
      721 acccggatgc ctcgctcggc ggcctcggtt tccgagagcc cgacgtgggc gacttccgga
      781 tccgtgaacg tcacccaggg caccaccgag tagtcgacct tgaacttgcg gaaccggccg
      841 aacagcgcgt tgaccgccgc gtaccaggcc tgatgagagg ccatgtgagt gaactggtag
      901 ggacccacca cgtcgccgca gccgtagacg ttacgcaccg ccgtgcggag ataggcgtcc
      961 acctccagcg ttccgtctga ccgggtggcg atccccagcg cttcgagccc cagccccgcc
     1021 gtgttggcgc ggcgcccgac ggcaacgagc acccgatcga agccgatctg cacctcgccg
     1081 ccgtccgaat cggcatgtag caccccggcg ccatccgccg aggccaccac ccgcacggcg
     1141 cggtggccgg tgcggacgtc gatgccctca ccgcgcagcc tgcgctcgac gaagccggcg
     1201 acgtcgtcgt cctcccgggg catcaggccg ggcgccatgt cgatgagcgt gaccgccgag
     1261 cccagtcggg cgaaagcctg agccagctcg cagccgatcg gccctccacc catcaccaga
     1321 agccgctggg gcaggctctc gagctcccag agattatcgg aggtgagcgg ccgggcatcg
     1381 gcgagcccgg gaatgggcgg gacaacgggc tcagccccgg tggcgagaac gatgctgcgg
     1441 gcgctcaggc gctcatcgcc aacctgcacg tgatgccggt cgatcaggcg cgccgctccg
     1501 gccacgcagt tgacgccgag ttcccggtag cgggccatgg agtccttggg ttcgatggtc
     1561 gcgatgacct cgtgtacccg gcgcatgacc gactgaaagt ccacctgggg agtcaccgcc
     1621 tgcaccccgt agcgctgggc ggtggcctga taatgagcaa tcttggccga gcggatcagc
     1681 gtcttgctcg gaacgcaccc cgtgttgagg cagtcgccgc ccatggcgcc ctgctcgata
     1741 agggtgaccc gggcccggac cgtggccgcg atcagggcga cgatgaggcc ggccgagcct
     1801 gcgccgatca cgatcaggtt gttgtcgtac ttcgccatgc cctgtgaccc ccacggaaac
     1861 tgcagctcat cgcgccgccc gggcgcgctc gagccatccc atggtcttgc gcgcgatcag
     1921 cggaaacaac ccgagcaagc tgagggctgc gatgagcccc gggctgagca cgtcggcaac
     1981 ggtgtccacg tttgccagct gagtgcccgc aaatacgaag acgagcgtgc cggccagcat
     2041 tcccacctgg ctgacccagt agaactgcca ggtaccgatg gcggtgagcc ccatgaccag
     2101 attgacgatg aagaacggaa acagcggcac catgcgcacg ctgaaaaggt agaacgcgcc
     2161 gtcgcgtgcc aggccatcgt tgaccacccg aagctgggcg gcaaagcgcg cgtgcaccca
     2221 gtcgcgcagg agcgtgcggg agatgagcat ggccagggtt gcgcccgcgc tgctggcaaa
     2281 ggacgtgata agggtgcccc agaacaggcc gaacacggcc cctgcagcca gcgtcatgat
     2341 cgcagcgcca ggcagcgaga gcgccgtgac cgcgatgtag aagagaaaga agccaaggat
     2401 gctcaccagc gcgttttctc tcacgaaaac gacggcatcg tggtgaacgc ccttgagaaa
     2461 ggagaggctg agatagtcgt tcaggccggt caggaaaaag g
//
LOCUS       DBNHQR010000056         3510 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_131701, whole genome shotgun sequence.
ACCESSION   DBNHQR010000056 DBNHQR010000000
VERSION     DBNHQR010000056.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_131701"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="AC16VR_01240"
     CDS             complement(<1..143)
                     /locus_tag="AC16VR_01240"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003135337.1"
                     /note="composes the biotin carboxyl carrier protein
                     subunit of the acetyl-CoA carboxylase complex, the enzyme
                     that catalyzes the carboxylation of acetyl-CoA to
                     malonyl-CoA, which in turn controls the rate of fatty acid
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HIR6009804.1"
                     /translation="MDLRKIKKLIELVEESGIAELEVRDGEEAIRIARPVAGTVPAPV
                     AQVR"
     gene            complement(156..587)
                     /gene="aroQ"
                     /locus_tag="AC16VR_01245"
     CDS             complement(156..587)
                     /gene="aroQ"
                     /locus_tag="AC16VR_01245"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HIR6009805.1"
                     /translation="MARFLVLHGPNLNLLGTREPEIYGTDTLEDINSRLEALAREAGN
                     EATCLQSNAEHELVDAVHGAKTQGVEGIVINPGAFTHTSVALRDALLGVGIPFIEVHL
                     SNVHAREEFRRQSYLSDVARGVITGLGAKGYEFALLAAMAR"
     gene            complement(675..2585)
                     /gene="dsbD"
                     /locus_tag="AC16VR_01250"
     CDS             complement(675..2585)
                     /gene="dsbD"
                     /locus_tag="AC16VR_01250"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     [NAD(P)H] activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HIR6009806.1"
                     /translation="MRRFWPGLGAAAIGFTLLLAAPGAAASALDDISTGAAGWLSEQP
                     TFLPVDEAFAMSAEVGPDGAILVRWDIADGYYLYRHRFTFEAREPAGGGAIAVLGAPE
                     IPPGKAKVDEYFGEVEVYYGRATARLPVQGGATVEVGIGYQGCADLGLCYPPETRWVT
                     LTMPGAPGAISGGDAGSAPGSPAATVQTEERALAGLLAEGSLLTALLLFFVAGVGLTF
                     TPCVLPMVPILSSIIVGDSEKLTRSRGITLSLAYVLGMAFTYAIIGTLMGMFGASLNM
                     QAALQSPPVLVGFALVFAALSLSMFGLYELRLPASWQSAIDAVGSRTGGGKHLSVAVM
                     GSLSSLVVSPCVSAPLAGALIYLSATGDAVLGGGALLALGLGMGVPLLLIGASGGHML
                     PRAGEWMNAVKILFGVLLLAVAVWLLERVVPGPVNLALWAALAIGAGGYLGLRAWSPR
                     GMVSGAVRAAGLLGVVYGALLLVGAAGGGNDPLRPLAGLTAWDPAHQPGEALEWQPVD
                     DVAGLRAAVLAASREGKPVMLDLYADWCISCKVLERSVFPKPEVAARLSRFNLLRADV
                     TANDDHHKALLDELGVFGPPSMVFFAEDGRKLAEVTVQGEIGADALAAHLGAVLERVG
                     SNNFSEIAVNFR"
     gene            2799..3419
                     /locus_tag="AC16VR_01255"
     CDS             2799..3419
                     /locus_tag="AC16VR_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009807.1"
                     /translation="MPEGARADPLTEPAPAPAPHAAEIDALLLQANDAMERGQYINPA
                     RGSALGLYDRVLALNPGNAEARRGLELIAQHYLDLASAAVRRRQLTRARSMVDWARIV
                     DAEHPGIAAAEAQIRLLAEARRVRLPVAGSQLRDRSDPLARSLAELGSRARNGNCLAI
                     IRSPSDASGRWIYQQMSRAPGTARIRADIEIGSPPMVELLCFENDA"
BASE COUNT          637 a         1254 c         1117 g          502 t
ORIGIN      
        1 cgcacctggg caaccggcgc cggcacggtg cccgcgaccg gccgggcgat gcggatggcc
       61 tcttccccgt cgcgcacctc gagctccgcg atgcccgact cctccaccag ctctatgagc
      121 ttcttgatct tcctgagatc cacgatcagc ctctcctatc gcgccatggc agccaggagg
      181 gcgaactcgt atcccttcgc cccgagcccg gtgatcacgc cgcgcgcaac gtccgacagg
      241 taggattgac gtcgaaactc ctctcgggca tgcacgttgg aaaggtgcac ctcgatgaag
      301 ggaatgccca ccccgagcag ggcgtcccgc aaggccacgc tggtatgggt gaaggcgccg
      361 ggattgatga cgataccctc cacgccctgg gtcttggccc cgtgcacggc gtcaacgagc
      421 tcgtgctcgg cgttgctctg caggcacgtg gcctcgttgc ccgcctcccg ggccagggcc
      481 tccagcctcg aattgatgtc ctcgagggtg tcggtgccgt agatctcggg ttcccgggtg
      541 ccaagcaggt tcaggttggg gccatgaagc acgaggaatc gggccatcgc aggactccag
      601 acgtcacagg gcgccgagtt tgcagaaacc cacgcggaaa gacaatacgg ataaaatgtg
      661 aatttggacg ttaattaacg gaagttaacc gctatttcag agaagttatt tgatccaacc
      721 cgttccagga ccgcccccag atgggccgcc agcgcgtccg cgccgatttc gccctgcacg
      781 gtaacttcgg ctaatttgcg accatcttca gcgaaaaaga ccatgctggg aggcccgaag
      841 acgccaagct cgtccaggag cgctttgtgg tgatcgtcat tcgccgtcac gtccgcccgc
      901 agcaggttga accgcgacaa cctggctgcc acctccggct tcgggaaaac gctgcgctcc
      961 agcactttgc aggagatgca ccagtcggcg tagagatcga gcatcaccgg cttgccctcg
     1021 cggctggcag ccagcacggc ggcacgcagc cccgccacgt catccacggg ctgccactcg
     1081 agcgcctcgc caggctgatg ggccggatcc catgcggtga ggcccgccag gggcctgagc
     1141 gggtcgttgc cgccgccagc cgccccgacg agcagcaaag cgccgtagac gacgcccagc
     1201 agccccgcgg ccctgacggc gcccgacacc atgccccggg ggctccaggc acgcaggccc
     1261 aggtagccgc cggcgccaat ggccagcgcc gcccacagcg cgaggttcac cgggcccggc
     1321 accactcgct ccagcagcca tacggccacg gccagcagca gcacgccgaa caggattttc
     1381 acagcgttca tccactcgcc ggcacgaggc agcatgtgac ccccgctcgc gccgatcaga
     1441 agcagcggaa cccccatgcc gagccccagg gccaggaggg cgccgcctcc gagcaccgca
     1501 tctccggtcg cgctcaggta aatgagggca ccggccagcg gggccgatac gcagggcgag
     1561 accaccaggc tggacagaga gcccatgacg gccacgctga ggtgtttgcc gccgcccgtc
     1621 cgcgatccca cggcgtcgat ggccgactgc cagcttgccg ggagccgcag ctcgtagagg
     1681 ccgaacatcg acagagacaa cgcggcgaaa accagggcga accccaccag cacggggggc
     1741 gactgcagcg cggcctgcat gttcaggctg gcaccgaaca ttcccatcag agtgccgatg
     1801 atcgcgtagg tgaaggccat cccgagcacg tatgccagcg agagcgtgat gccacggctt
     1861 cgcgtgagct tctcggaatc gccgacgatg atgctggaaa gaatcggcac catgggaagg
     1921 acgcagggag tgaaagtgag ccccaccccc gccacgaaaa agagcagaag cgcggtcagc
     1981 aggctgccct cggcgagcag gccggcaagg gcccgttcct ccgtctgaac cgtcgcagcg
     2041 gggctgccag gcgcgctgcc ggcatctccg ccggatatcg cgcccggggc gcccggcatt
     2101 gtcagggtca cccaccgggt ctccggggga tagcaaagac ccagatccgc gcatccctga
     2161 tagccgatgc ccacctccac ggttgcgccg ccctgaacgg gcagccgggc agtggctctg
     2221 ccgtagtaca cctccacctc gccgaagtac tcgtcgacct tggccttgcc tggcgggatc
     2281 tccggggcgc cgagaacggc aatcgcccca ccacccgccg gctcgcgggc ctcgaaggtg
     2341 aatcggtgcc ggtagaggta gtagccgtcc gcgatgtccc agcgaacgag tatcgcgccg
     2401 tcgggcccga cctcggcgct catggcaaac gcctcgtcca ccgggagaaa cgtcggctgc
     2461 tcggatagcc acccggcggc ccccgtggag atgtcgtcca gggccgacgc ggccgcaccg
     2521 ggcgcggcca gcagcaaagt aaaaccaatc gccgcggcgc caagccccgg ccagaatcgc
     2581 ctcattgttc gacggactct catcacctca attctatccg acagctcgac cgcattggag
     2641 ttcgcccgca gacccggcaa tatagccgtt cccttggccc gcagaagtga actggtcggt
     2701 tgcttcccag tgaacgaaca ctcccagcgc tctggctcgc cctgatgctc tgggtggtgg
     2761 acgccgctgg actctcggcg caggccggag gtgattcctt gcccgaagga gcgcgggccg
     2821 accctctcac cgagcccgcg cccgccccgg cccctcacgc ggccgagata gacgcgctgc
     2881 tgctgcaggc caacgacgca atggaacggg gacagtacat caaccccgcc cgcggaagcg
     2941 ccctgggcct ctacgaccgg gtactggcgc tcaatccggg caatgccgag gcgcgcaggg
     3001 gcctggagct catcgctcag cattatctcg acctcgccag cgccgcagta aggcggcgtc
     3061 agctgacccg cgcccggtcc atggtcgact gggccagaat cgtggacgcc gaacatcccg
     3121 gcatcgccgc ggccgaggcg cagatccgcc tgctcgcaga ggcgaggcgc gtgcgcctgc
     3181 cggtggcagg cagccagctg cgcgatcgca gcgacccgct ggctcggtcg ttggcggagc
     3241 tcggatcacg cgcgcgcaac ggaaactgcc tggcgatcat ccggtcgccc agcgacgcct
     3301 cgggacgctg gatataccag cagatgagcc gcgctccggg aactgcgcgc atccgggccg
     3361 acatcgagat cggctcaccg cccatggtgg aactgctttg cttcgaaaac gacgcctgat
     3421 gcccgatgcc gccatattgg ccgccgcgct ggccgccgcg ccggttgcca tcccggccag
     3481 cagcgcgccc gccctggcgg gttccgagct
//
LOCUS       DBNHQR010000057         2921 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_828643, whole genome shotgun sequence.
ACCESSION   DBNHQR010000057 DBNHQR010000000
VERSION     DBNHQR010000057.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_828643"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..634)
                     /locus_tag="AC16VR_01260"
     CDS             complement(98..634)
                     /locus_tag="AC16VR_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009808.1"
                     /translation="MTRIAALVVLFAPIASLQADHHGALIAQARSAAPELVSADATVM
                     YRGEVLVEGTNGWVCMPETLPDDNSPMCNDAVWMGLMQAMDAGEEYEVERIGISYMLQ
                     GDAGVSNSNPAHADHVNAPDFIKEGAHLMIAVPRAMLEGITDDPHGGGPYVMWKDTPY
                     AHIMVPLEDANRSAPPAQ"
     gene            811..1212
                     /locus_tag="AC16VR_01265"
     CDS             811..1212
                     /locus_tag="AC16VR_01265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HIR6009809.1"
                     /translation="MATFLAKIKIFEGKEAEFEETARMMFRETHANEPTCRRYEYWRG
                     AEPRTYYCMESFDDFVGFMEHQVSPHHEAPDFASMLEEIELEWIDPIQGACDLTSTNP
                     KDLPEDASDLMKEYAATHPLVMQDWWLNLRK"
     gene            1225..2334
                     /locus_tag="AC16VR_01270"
     CDS             1225..2334
                     /locus_tag="AC16VR_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012074704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase [NAD(P)+] activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HIR6009810.1"
                     /translation="MKTRAAVAFEPKKPLEIVEVDLEGPRTGEVLIEIKATGICHTDA
                     YTLDGLDSEGVFPSILGHEGAGVVVETGPDVTSVAPGDHVIPLYTPECRQCKSCLSRK
                     TNLCTAIRATQGQGLMPDGTSRFSFEGKPIHHYMGCSTFSNYTVVPEIAVAKIRADAP
                     FDKACYVGCGVTTGVGAVVNTAKVEPGANCIVFGLGGIGLNVIQGLRMVGASRIIGVD
                     VNPRKEAWGRQFGMTHFVDPGTVDGDLVAHLVELTDGGADYTFDCTGNTDVMRTALEA
                     CHRGWGTSVIIGVAEAGREIATRPFQLVTGRVWKGTAFGGARGRTDTPKVVDWYMDGK
                     IEIDPMITHTLPLERINEAFDLMHRGESIRSVVVY"
     gene            2499..>2921
                     /locus_tag="AC16VR_01275"
     CDS             2499..>2921
                     /locus_tag="AC16VR_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6009811.1"
                     /translation="MTANSPTGDYVQMDSVDWIPFPEGLCEGPITWKLLHVSPESGAW
                     TAIFDCPAGTSFAKHIHMGPGEYFLTKGRMNVRGGSAEGGATAVAPGYGYEECNAQHD
                     HTEFPEDSEFYMTFLGPLNFIDDDGNTIALVGCKEVLDA"
BASE COUNT          571 a          941 c          926 g          483 t
ORIGIN      
        1 tatctaaaca tgtcgacggc gccggatgaa aatctcaacg gcaatggacg ccggccgggg
       61 ccggcatcag ccccgccggg cgctggcacc gggcgtccta ttgcgccggg ggagccgacc
      121 ggtttgcgtc ctccagcggc accatgatgt gcgcgtaggg cgtgtccttc cacatcacgt
      181 agggcccacc cccgtgcggg tcatcggtaa tgccctcgag catcgcgcgc gggacggcaa
      241 tcatgagatg cgcgccttcc ttgatgaaat caggggcatt cacatggtcg gcatgcgcag
      301 ggttcgagtt gctcacgcca gcatctccct ggagcatgta ggagatgccg atgcgctcca
      361 cctcgtattc ttcaccagcg tccattgcct gcatcaggcc catccagacc gcatcgttgc
      421 acatgggcga gttgtcgtcc ggcagcgtct ccggcatgca gacccagccg ttggtccctt
      481 cgaccagcac ctcgccgcga tacatcacag tcgcatcggc gctcaccagt tcgggggccg
      541 ccgaccgggc ctgggcgatg agcgccccgt ggtggtccgc ctgcagcgac gcgatcggcg
      601 caaacaacac aaccagggca gcgatccggg tcatagctga tctcatcatc tgtctccttg
      661 gaaaatcgac gctccagacg ttacgcccgg cggcaccctg gggcaaggcg aatcacccag
      721 ttgcccatgt ccccggtcgc caaacgagga agggtgcggt tatccttgct agaccgataa
      781 atcatcatcc agggagcatg aggggggacc atggccacgt ttctggcaaa gattaaaatt
      841 ttcgagggca aggaggctga gttcgaggag accgcgcgga tgatgttccg cgagacccat
      901 gccaacgagc ccacctgccg acgttacgag tactggcggg gcgcggagcc gcgcacgtac
      961 tactgcatgg agtcgttcga cgacttcgtc ggcttcatgg agcatcaggt ttcgccccac
     1021 cacgaagcac cggactttgc ctcgatgctc gaagagatcg agctggagtg gatcgatccc
     1081 atccagggcg cctgcgacct gacgtccacc aatcccaaag acctgcccga ggatgccagc
     1141 gatctcatga aagagtatgc ggcaacccac cccctggtca tgcaggactg gtggctgaac
     1201 ctccgcaagt aacagggagc aaatatgaag acgcgtgcgg ccgtggcctt cgagcccaag
     1261 aaaccgctgg agatcgtgga ggtcgacctg gagggtccca gaaccggcga ggtgctcatc
     1321 gagatcaagg ccaccggcat ctgccacacg gacgcctaca ccctcgatgg gctcgattcc
     1381 gaaggggtct ttccgtccat cctcggccac gagggcgccg gcgtcgtggt cgagacaggc
     1441 ccggatgtca cttcggttgc gcccggcgat cacgtcattc ccctgtacac gcccgagtgc
     1501 cggcagtgca aatcctgcct gtcccgaaag acgaatctgt gcacggccat ccgcgccacg
     1561 caaggccaag gcctgatgcc cgacggcacc tcccgattct cgttcgaggg caagcccatc
     1621 catcactaca tgggctgctc gacgttctcc aactacacgg tggtccccga gatcgcggtg
     1681 gcgaagatcc gcgccgacgc ccccttcgac aaggcctgct acgtgggctg cggcgtcacc
     1741 accggggtgg gggccgtggt gaacacggcc aaggtcgagc ccggcgccaa ctgcatcgtc
     1801 ttcggcctcg gcggcatcgg cctcaacgtg attcagggcc tgcgcatggt gggcgccagc
     1861 cggatcatcg gggtggacgt caacccgcgc aaggaggcgt ggggacgcca gttcggcatg
     1921 acgcacttcg tggacccggg gacggtggac ggcgatctgg tcgcgcacct ggtggagctc
     1981 accgacggcg gcgcggacta caccttcgac tgcacgggca acaccgacgt catgcgcacg
     2041 gcgctggaag cctgccaccg cggctgggga accagcgtca tcatcggcgt ggccgaagcg
     2101 ggcagggaga tcgccacgcg tccgttccag ctggtcaccg ggcgggtctg gaaaggcacc
     2161 gccttcggcg gcgccagagg gcggaccgac acgccgaagg tggtggactg gtacatggac
     2221 gggaagatcg agatcgatcc catgatcacc cacacgctgc cgctcgagcg catcaacgag
     2281 gcgttcgacc tgatgcaccg gggtgaaagc atccgctccg tggtcgttta ctgaccggac
     2341 agctctgatc gcgaagggag acgggcgccg ccaacgcggt gctaaagcat tgacgcccac
     2401 ctgagcgcgg gacaaactgc agtggagggt gtccgagtct tcatcggacg agtcagcaaa
     2461 gaacgcagaa gctctgcgtt caggttggga ggatcgacat gacagcaaac agccccactg
     2521 gggattacgt ccagatggat tccgtggact ggattccttt tccggaaggc ctttgcgaag
     2581 gccccatcac ctggaaactg ctccacgtct caccggaatc cggcgcgtgg acggccatct
     2641 tcgactgccc cgcaggcacg tcgttcgcca agcacattca catgggcccc ggcgagtact
     2701 tcctcaccaa gggcaggatg aacgtgcgcg gaggctcggc cgaaggcggt gctaccgccg
     2761 tcgcgcccgg ctacggctac gaggagtgca acgcccagca cgaccacacg gagtttcccg
     2821 aggacagcga gttctacatg acctttctgg ggccgctgaa cttcatcgac gatgacggca
     2881 acaccatcgc gctggtgggg tgcaaagagg ttctggacgc c
//
LOCUS       DBNHQR010000058         4583 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1208908, whole genome shotgun sequence.
ACCESSION   DBNHQR010000058 DBNHQR010000000
VERSION     DBNHQR010000058.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1208908"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..1256)
                     /locus_tag="AC16VR_01280"
     CDS             complement(153..1256)
                     /locus_tag="AC16VR_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014821026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR6009812.1"
                     /translation="MRFLEVIGGWINRHFSNEEAIYLVVLIVIISTVVFIFGSMLAPV
                     LIGLIVAFLLQGLVKRLVDAGLGGGLAVYLTFIIFLGALIALVLFVVPIVWQQLRALV
                     DALPNGIVQVQALIRDLANTSPGGLVTPEQVDKWLGSLTGELGNLSRRLLNTLLTQVP
                     SVVALMIYTVIVPFSVFFFLKDRDELMRWFLSLLPEERPLLDRVGAEMNLQLANYVRG
                     KFVEILIVGVTTYVTFVLFGLNYAALLAVLVGLSVLIPFVGAAVVTIPVALVAVVQFG
                     WSLPLAGVLGAYLIIQFLDGNFLVPLLISSAVNLHPITIISAVLAFGGLWGIWGVLFA
                     IPLATLVKSIYNAWPRQEELAEASGDGLVEVDG"
     gene            complement(1256..1489)
                     /locus_tag="AC16VR_01285"
     CDS             complement(1256..1489)
                     /locus_tag="AC16VR_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003441329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HIR6009813.1"
                     /translation="MTEPVVVDAKGLNCPMPLLKAKKALNELSPGELVKVLATDPGSV
                     RDFEVFSRQSGHALLESLREDDTYIYLLRKKPG"
     gene            1618..3096
                     /locus_tag="AC16VR_01290"
     CDS             1618..3096
                     /locus_tag="AC16VR_01290"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HIR6009814.1"
                     /translation="MQDSRAKQRRLSGAGAVIRLGIGAALCLLTAAASTDVSDDLPVL
                     GDASSSMISPELERQIGRDFLKQVHAGLPTISDPVLKHFVTRQLTDLLQYSQVRENLL
                     GVVLIDSDQINAFAAPGGVVGVNLGLLLHAQDVHEYSSVMAHELAHLSQRHFARGVEE
                     ARSKTLPTIASLLAAIMIGAVGGGEAGMAAISASQAASQSSQLRYSRGREQEADRIGL
                     NTIVRAGMDPDGMARMFERMQRNYRFSRTPPEFLLTHPLSETRISDAKSQAREYPQRD
                     YADSPDYAMMRVRAQIYYAPNAAGAVAQFEKAVRDDPESVPARYGLALAQSRAGLHAE
                     AIAGGDALFTADPQKILYIASYAELLIEAEKLDQATRLLSHHLALNPDNATLAAMYSE
                     ALIRDQRYREAEAVLERQSVVNKDDVDVWYNLAEVSGKAGNIIGVHRARAEYFALHGA
                     YQRAIQHLEYARRLVREDNQQLHAMLDQRINDMRMALRMRRS"
     gene            complement(3093..4142)
                     /gene="nadA"
                     /locus_tag="AC16VR_01295"
     CDS             complement(3093..4142)
                     /gene="nadA"
                     /locus_tag="AC16VR_01295"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003292836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HIR6009815.1"
                     /translation="MSEATLPDYTLSADRVPVYEPEVLGPERVADLKSEIADLLAARG
                     AVLVAHYYVDGHVQDLAEETGGFVSDSLEMARFGHDHAAETLVVAGVRFMGETAKILS
                     PEKRVIMPTLEAECSLDIGCPPDDFEAFCKANPDRTVVVYANTSARVKAMSDWVVTSS
                     VALPIVEMLDARGEKILWAPDKHLGHYIQTQTRADMLLWSGACVVHEEFKSKALFDLK
                     AVYPEAGVLVHPESPAAVISIADVVGSTSQIIKAAQELPHDTFIVATDRGIFHKLRQR
                     NPDKRFIEAPTAGEGATCRSCAHCPWMAMNELSTLAAMLRSEGAMTANEIHVDPEIGR
                     RAMIPLKRMLDFKVP"
     gene            4254..>4583
                     /locus_tag="AC16VR_01300"
     CDS             4254..>4583
                     /locus_tag="AC16VR_01300"
                     /inference="COORDINATES: protein motif:HMM:NF014816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6009816.1"
                     /translation="MYTLYEMGDSGNCYKIRLLMHQLDVPFERVPTDILQGRSRTESF
                     LALNPNGKVPLLVLPDGRPLAESNAILSYLARGTAYLPDEAYEQALVLQWLFFEQYSH
                     EPYIATNR"
BASE COUNT          925 a         1484 c         1418 g          756 t
ORIGIN      
        1 agggcggtcc agtcgacctc catccccgcg gtcatgggcg tgatcagggc aacgatgcta
       61 cctgtgaaca tatcgctctc tagctgggcg acccggccct agggacgcgg ggagcagaac
      121 cgcgccatgg tacttgagcg gcgcaacggg gctcaaccat cgacttcgac caggccgtcc
      181 ccgctggctt ccgccaactc ctcctggcgc ggccaggcgt tgtagatgga cttcaccagc
      241 gtcgccagag gaatggcaaa aagaaccccc catatccccc acaggccgcc gaaagcgaga
      301 acggcagaga tgatggtgat ggggtgcagg ttgacggcgc tggaaatcag caacgggacc
      361 agaaaattgc cgtccaggaa ctgaatgatc aggtacgcgc ccagcacgcc cgccaggggc
      421 agggaccatc cgaactgcac gacggccacc agcgcgacgg ggatcgtcac cacggccgcc
      481 ccgacaaacg gaatcagcac cgagagcccc acgagcaccg ctaggagcgc ggcgtaattg
      541 agaccgaaca gcacgaaggt cacgtaggtg gtcacgccaa cgatgaggat ctcgacaaac
      601 ttgccgcgaa cgtagttggc cagctgaagg ttcatctccg cgcccacccg gtccagcagc
      661 gggcgctcct cggggagcag agagaggaac cagcgcatga gctcgtcacg atccttcagg
      721 aagaagaaca ccgagaaagg cacgatcacc gtgtagatca tcagcgccac cacggacggg
      781 acctgggtga ggagcgtgtt tagcagcctg cggctcaggt tgcccaactc tcccgtcagc
      841 gagcccagcc acttgtctac ctgctcaggc gtcaccagcc cacccggcga ggtgttggcc
      901 agatcacgga tgagggcctg cacctgcacg atcccgttcg gaagcgcgtc gaccagggca
      961 cgaagctgct gccagacgat gggcaccacg aagagaacga gggcgatcag ggcgccaagg
     1021 aagatgatga acgtcaggta aacggctaac ccgccgccga gccccgcgtc caccagacgt
     1081 ttcaccaaac cctggaggag aaaggcgacg atgagcccga tcagcaccgg ggccagcatc
     1141 gacccgaaga tgaacaccac ggtagagatg atgacgatga ggaccaccag gtagatggct
     1201 tcctcattgg agaagtgccg gttgatccag ccgcctatga cctctagaaa gcgcatcagc
     1261 ccggcttctt tcgcagcagg tagatgtagg tatcgtcctc gcgaagcgat tccagcagag
     1321 cgtggccgct ctgccgcgaa aacacctcga agtcgcgaac cgatccggga tcggtggcga
     1381 ggaccttcac aagctcgccc ggggagagct cgttgagagc tttcttggct ttaaggagcg
     1441 gcatcgggca gttcaggccc ttggcatcga ccactacggg ttcggtcatg ttcggttcag
     1501 cgcacggatg agcggcagca ggatgcctat cataagactt ttgaactttt cccgcccgca
     1561 ggtggctaaa accagagcag gttctgcggc acaccgccat ccagtatgat ctccagaatg
     1621 caagactccc gagcgaagca gcgacgtctg agtggcgccg gggccgtgat tcgcctcggc
     1681 atcggcgctg cgttgtgcct gctcacggcc gcagcctcca cggacgtcag cgacgacctg
     1741 cccgtgctcg gcgacgcctc ctcgagcatg atctccccgg aactcgaacg ccagatcggc
     1801 agggatttcc tcaagcaggt gcacgcgggc ctgccgacca tcagcgaccc cgttctcaaa
     1861 cacttcgtca cgcgccagct gaccgacctg cttcaatact cccaggtcag ggaaaacctg
     1921 ctgggcgtgg tgctcatcga cagcgatcag atcaacgcct ttgccgcccc cggcggcgtg
     1981 gtgggcgtca acctgggact tctgctgcat gcccaggacg tgcacgaata ctcctcggtc
     2041 atggcacacg agcttgctca cctcagccag cgccacttcg ccaggggcgt cgaggaggcc
     2101 cgcagcaaaa ccctgcccac catcgcctcc ctgctggccg ccatcatgat cggcgccgtg
     2161 ggcgggggcg aagcgggcat ggcggccata tctgcttccc aggcggccag ccagtccagc
     2221 cagctgcgct acagccgggg ccgcgagcag gaagccgacc ggatcggcct caacaccatc
     2281 gtccgggccg gcatggatcc ggacggcatg gcgcgaatgt tcgagaggat gcagcgcaac
     2341 taccgctttt cccgcacgcc gccggagttc ctgctgaccc acccgttgag cgagacccgc
     2401 atctcggacg cgaaaagcca ggcccgcgag tacccccaac gcgactacgc cgactcaccc
     2461 gactacgcca tgatgcgggt ccgcgcccag atctactacg cgcccaacgc cgccggggcc
     2521 gtggcccagt tcgagaaggc tgtgcgggac gatcccgagt cggtccccgc gcgctatggg
     2581 ctggccctgg cgcagagcag agcgggtctg cacgccgagg ccatcgccgg cggtgatgct
     2641 ctgttcaccg ccgaccctca gaaaattctc tacatcgcgt catacgcaga gctgctcatc
     2701 gaggcggaga aactcgatca ggcgacccgt ctgctgtcgc atcacctggc cctcaacccg
     2761 gacaacgcca ccctggcggc catgtacagc gaggcgctca tcagggacca gcggtaccgg
     2821 gaggctgagg cggtgctgga gcggcaaagc gtcgtcaaca aggacgacgt ggacgtgtgg
     2881 tacaacctcg ccgaggtatc aggcaaagcg ggcaacatca tcggcgtgca ccgggctcgc
     2941 gccgagtact tcgcgctgca cggcgcctac cagcgggcca tccagcatct ggaatacgcg
     3001 cgccgcctgg tgcgggaaga caaccagcag cttcacgcca tgctggatca gcgcatcaac
     3061 gacatgcgga tggcgctgcg gatgcggcgg tcctagggca ccttgaagtc gagcatcctc
     3121 ttgagcggga tcatcgcccg acgaccgatc tcagggtcca catgaatctc gttggccgtc
     3181 atcgcccctt cgctgcgcag catggccgcc agggtggata actcattcat cgccatccaa
     3241 gggcagtggg cacagctgcg gcaggttgcg ccctcgccgg ccgtgggcgc ttctatgaat
     3301 cgcttgtccg ggttgcgctg gcgaagcttg tggaagatgc cgcggtcggt ggccacgatg
     3361 aaggtgtcgt ggggcagctc ctgggctgcc ttgatgatct ggctggtcga gcccaccacg
     3421 tcggctatgg aaatcaccgc ggccggtgac tcggggtgca cgaggacgcc cgcctccgga
     3481 taaacggcct ttaggtcgaa gagcgccttc gacttgaact cttcgtggac cacacaggcg
     3541 ccgctccaca gcagcatgtc ggcgcgggtc tgagtctgaa tgtaatgtcc cagatgcttg
     3601 tcgggggccc agagtatctt ttcacctcgg gcgtcgagca tctccacgat gggcagggcc
     3661 acgctgctgg tcaccaccca gtcggacatc gctttgaccc gggccgaggt attggcgtag
     3721 acgaccaccg tccggtcagg attggctttg cagaaggcct cgaagtcgtc cggcgggcag
     3781 ccgatgtcca gagagcactc ggcctcgagc gtcggcatga tcacccgctt ctctggcgag
     3841 agaatcttgg cggtctcgcc catgaaacgc acgcccgcga ccaccagcgt ctcagcggcg
     3901 tggtcgtgcc cgaatcgggc catttccagc gagtccgaga cgaacccgcc cgtttcctct
     3961 gcgaggtcct gaacgtgacc gtccacgtag taatgggcca ccagcacggc gccgcgggcg
     4021 gcgagcagat cggcaatctc ggatttcaga tctgcaaccc gttccggccc gagcacctcc
     4081 ggctcgtaga cgggtacccg gtccgcggag agggtgtagt ctggcagcgt agcttcgctc
     4141 atgacatcga gaatattgga agcgcaggga aaatcacagt tcccgtgcaa ccgtcgcctg
     4201 tttatatgac catcttttta ttggggcgaa tcgacgaaaa ccaaggaggt tcgatgtaca
     4261 cgctttacga gatgggagac tcgggaaact gttataagat ccggctattg atgcaccagc
     4321 tggatgttcc tttcgagcga gttcccaccg acatcctcca ggggcggtcg cgcacggaat
     4381 cgttcctggc gctgaatccc aatggcaagg tcccgctgct cgttctacct gacgggcgcc
     4441 cgctggcgga gtcaaacgcc attctgagct accttgcgcg aggtacggcc tacctcccgg
     4501 acgaggctta cgagcaggcg ctggtgctgc aatggctctt cttcgagcag tacagccatg
     4561 agccctatat cgccaccaac cga
//
LOCUS       DBNHQR010000059         4378 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1462211, whole genome shotgun sequence.
ACCESSION   DBNHQR010000059 DBNHQR010000000
VERSION     DBNHQR010000059.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4378)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1462211"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..558
                     /locus_tag="AC16VR_01305"
     CDS             175..558
                     /locus_tag="AC16VR_01305"
                     /inference="COORDINATES: protein motif:HMM:NF024030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3775 domain-containing protein"
                     /protein_id="HIR6009817.1"
                     /translation="MLELNPESVAQIAEKAREFQSEEILLPPEPPPVPVDDTAFEYAA
                     EWHGDPGYNDLKDAIEDLEPDQQSVLVALMWLGRGDYGIEEWNEALAYAEEVHTDATA
                     DYLIATPLLADYLLGGLEMHGYEPE"
     gene            651..1991
                     /locus_tag="AC16VR_01310"
     CDS             651..1991
                     /locus_tag="AC16VR_01310"
                     /inference="COORDINATES: protein motif:HMM:NF016150.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HIR6009818.1"
                     /translation="MSEVTTSVRITTVISGPVRRSDRIDSLDVMRGVALLGILLMNII
                     AFGLPFATYYNPSVAGIDNPANLWAYIVNWLFFEGTMRALFSMLFGAGMVLMTTRAEE
                     RGAGIEAADVYYRRLLWLILFGVIDAYLLLWVGDILFLYGIAGLFLFVFRGLAPRTLF
                     LIGVAMLVVLGIKNVNASNELAELRQVTAEVESIRAAGGEPSREQLAAIDHLEEIRAE
                     FEPDPEQIQEQVEARHSGYIDQVKYLVPLNIAAQSTLTYTLGIWDVLMMMFMGMALIK
                     WGVFSAARSWGFYGAMVVVGYGIGLPINWYEVRMAIESGFAIEWSGIQYVSTYQIGRA
                     GTAAGHIGLIMMICKSGLFGFITKPLAAVGRMALTNYLAHSVICMFLFTGVGLAWFGE
                     LERYELYYVVGGIWLFQLIVSPIWLRYFRFGPMEWLWRSLTYAQTQPMRRSPPG"
     gene            complement(2044..4089)
                     /gene="fusA"
                     /locus_tag="AC16VR_01315"
     CDS             complement(2044..4089)
                     /gene="fusA"
                     /locus_tag="AC16VR_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR6009819.1"
                     /translation="MAYTTADIRNVCLLGPAEAGKTQLAEALLYAGGAIAQCGQVAKG
                     NTVTDHSERERAIGHSLYPAVCHTDHAGIHVNVVDTPGYRDLYGRALAVLPAVETAVI
                     VINAEKGIEEMTRRMMEAAQAQHLCRMIVVNKIDSAEADLAGLVGEIQEAFGGECLPI
                     NLPASHGDGVVDCYFHPSGGETVFGSVEQAHTRLIDQVVEVDDELMELYLEQGDDLAP
                     EQLHEPFERALREGHLIPVCFVSARTGVGVADLLNVFERLMPNPEEGNPPEFLKGEGA
                     EAQPVSVVPDPEQHVVAHVVQVDVDPFKGRLAVFRIHQGSIRPGMQLYVGDARKPIKV
                     NQLLKLNGNGHSKVEVGLPGDICAIPRVDEAHYDAVLHDSHDEDHFHLRSEPLPPPMF
                     GLAIQPGNDADAQKTSDALHIMEAEDPSLQIEHVAALNEIVLRGLGELHLRSVLEEIE
                     ARHDLRVETALPSIAYRETITKPAQGHCRHKKQTGGAGQFGEVFLRIEPLPPGGGFEF
                     VSEVVGGAIPSQFIPAVETGVRLVLDGGAVAGYPLQDVKVTVYDGKHHSVDSKEIAFV
                     QAGRKAFVDAIRSASPIVMEPVVHATFTVPEDCVGGVSGDLAGMRGSVRGTSVLADGR
                     IEISGEAPLKDLQAYHSRLKSLTGGVGSFDMAFSRYAQVTPQTAQELMGAFAGSDEE"
BASE COUNT          749 a         1441 c         1396 g          792 t
ORIGIN      
        1 taccgtcgag gtaagcctca ggcctcgcag attctccgtc atgatgcgtc ctcctttctt
       61 cgcccggaga ccgtacaccg ttatggccta tgtacaaccc ctgatttgct tgctccgtag
      121 aacggcaaga ataagcgctg gttggtgaca gaatgaaacg aggaggcagg cggtatgctc
      181 gaactcaatc ccgagagcgt ggcgcagatc gctgagaaag cgcgggagtt tcaatccgag
      241 gagatcctgc tgcccccgga gccgcccccc gttcccgtgg acgacacggc tttcgagtat
      301 gcggccgagt ggcacggcga tcccggctac aacgatctga aggacgccat cgaggatctc
      361 gaacccgacc agcagtcggt gctggtggcg ctgatgtggc tcggccgggg cgactacggc
      421 atcgaggagt ggaacgaagc gctcgcctac gccgaggagg tgcacaccga cgcgaccgcc
      481 gactacctga tcgcaacccc tcttctggcc gactacctgc tgggaggcct ggagatgcac
      541 ggctacgagc ccgaatagcg cggcgctccc acgacggccg ggttcacccg gttgccaaag
      601 ccgttcaatc cgccgaaact ccaggctgcg ggaggtttca ggagaggggc atgagcgagg
      661 tcaccacctc cgtcaggata acgaccgtga tttccggccc ggttcggcgc agcgaccgga
      721 tcgactcgct ggacgtcatg cgcggcgtgg cgcttctggg catcctgctc atgaacatca
      781 tcgcgttcgg attgccgttc gccacctact acaaccccag cgttgccggg atcgacaacc
      841 ccgccaacct ctgggcctac atcgtcaact ggctgttctt cgaaggcacg atgcgcgcgc
      901 tgttctccat gctgttcggc gcggggatgg tgctgatgac cacccgggcc gaggagcgcg
      961 gcgccggcat cgaagcggcc gacgtgtact atcggcgcct gctgtggctg atcctcttcg
     1021 gcgtcatcga cgcctacctg cttctttggg tcggcgacat tctcttcctg tacggcatcg
     1081 ccggcctctt tctcttcgtc ttccgcggcc ttgcgccccg caccctgttc ctcatcggcg
     1141 tggcgatgct cgtcgtgctc ggcatcaaga acgtcaacgc cagcaacgag ctcgctgagc
     1201 ttcgccaggt gacggcggag gtcgagtcga tccgagcagc cggcggcgag ccaagccggg
     1261 agcagctggc cgccattgac cacttagagg agatccgggc ggagttcgag cccgaccccg
     1321 agcagatcca ggagcaggtg gaggcgagac actccggcta catcgaccag gtgaaatacc
     1381 tcgttcccct gaacatcgcc gcgcagtcca cgctgaccta cacgctcggc atctgggacg
     1441 tgctgatgat gatgttcatg ggcatggcgc tgatcaaatg gggcgtattt tccgcggcgc
     1501 gcagctgggg cttttacggg gccatggtgg tggtcggcta cgggatcggc ctgcccatca
     1561 actggtacga agtccgcatg gccatcgagt caggcttcgc catcgagtgg tcggggattc
     1621 agtacgtctc cacctatcag ataggccggg cggggaccgc cgccgggcac atcggtctga
     1681 tcatgatgat ctgcaagtcc ggcctgttcg gcttcatcac gaagcccctg gccgccgtcg
     1741 ggcgcatggc gctgaccaac tatctggcac acagcgtgat ctgcatgttt ctgttcaccg
     1801 gggtcggcct ggcatggttc ggcgagctcg agcgctacga gctctactac gtggtgggcg
     1861 ggatctggct gttccagctc atcgtcagcc cgatctggct gaggtacttc cgcttcggcc
     1921 ccatggagtg gctctggcgg tcgctgacct acgcgcagac ccagcccatg cgccgctcac
     1981 cccccgggta aggcggcctc tccccgggct gcgggccgcg gcgccctcga cgttgcccgc
     2041 cggctactcc tcgtcgctgc ccgcaaacgc gcccatcagc tcctgggccg tctgcggcgt
     2101 cacctgggca tagcggctga acgccatgtc gaagctgccc acgccccccg tgagcgactt
     2161 gagcctggaa tgataggcct gcaggtcctt cagcggcgcc tcgccggaaa tctcgatccg
     2221 cccgtccgcc aggacgctgg ttccgcgcac ggagccgcgc atgccggcca gatctcccga
     2281 gacaccgccc acgcaatctt ccggcaccgt gaaggtggca tggacgacgg gctccatgac
     2341 gatgggcgac gcgctgcgaa tcgcgtcgac gaacgctttt ctgcccgcct gcacgaaggc
     2401 gatctccttg gagtccaccg agtggtgctt gccgtcgtag acggtcacct tgacgtcctg
     2461 caggggatag cccgcgacgg cacccccgtc gagaaccagc cgcacgccgg tttccacggc
     2521 gggaatgaac tgtgacggga tggctccgcc gacgacctcg ctgacgaact cgaaacctcc
     2581 gcccggcggc agcggctcga tccgcagaaa cacctcgccg aactggcccg cgccacccgt
     2641 ctgctttttg tgccggcaat ggccttgcgc cggtttcgtg atggtctccc ggtaggcgat
     2701 ggagggcagc gccgtttcga ccctgaggtc gtgacgcgct tcgatctctt cgagcaccga
     2761 gcgcagatgc agctcgccca gcccgcgcag cacgatctcg ttcaacgcgg cgacgtgctc
     2821 gatctgcagc gaaggatcct ccgcttccat gatgtgcagg gcgtccgacg ttttctgggc
     2881 gtcggcgtcg ttcccgggct ggattgcaag accgaacatg ggcggcggca gcggctcgga
     2941 gcgcaggtga aagtggtcct cgtcgtggga gtcgtgcaga acggcgtcgt agtgtgcttc
     3001 gtcgactcgc gggatggcgc agatatcccc cggcagaccc acctccacct tggagtggcc
     3061 gttgccgttg agcttcaaga gctggttgac cttgatgggc ttgcgggcgt cgccgacgta
     3121 gagctgcatg cccgggcgga tgctgccctg atggatccgg aacaccgcca ggcggccctt
     3181 gaaggggtcg acgtccacct ggacgacgtg ggccaccaca tgctgctcgg gatccggaac
     3241 cacgctgacg ggctgggcct cggcaccctc gcccttcagg aactcgggcg ggttaccctc
     3301 ttccggattc ggcatgagcc gctcgaagac gttcagcaga tcggcgacgc ccacgcccgt
     3361 gcgggcggag acgaagcaca cgggaatcag gtgaccttcc cgcagcgccc gctcgaaggg
     3421 ctcatgcagc tgctccggcg cgaggtcgtc gccctgctcc aggtagagct ccatgagctc
     3481 gtcatccacc tccaccacct gatcgatcag ccgggtgtgc gcttgttcca cgcttccgaa
     3541 cacggtttcc ccgccggagg gatggaaata gcagtccacc acaccgtcgc catgggaggc
     3601 gggcagattg ataggcaggc actcgccacc gaaagcctcc tgaatctcgc cgacgagccc
     3661 ggcaagatcg gcctcggcgc tgtcgatctt gttgacgacg atcatgcggc acagatgctg
     3721 ggcttgtgcc gcctccatca tgcgccgggt catctcctcg atgcccttct ccgcgttgat
     3781 gacgatgacg gcggtctcca ctgccgggag caccgccagg gctcggccgt aaaggtcccg
     3841 gtagccgggc gtgtccacga cgttcacgtg aatgccggcg tgatccgtat gacacaccgc
     3901 gggatacaga gagtggccga tggcgcgttc gcgttcgctg tggtcggtaa cggtgttgcc
     3961 cttggcgacc tgcccgcatt gggcgatggc gccccctgca tacagcagcg cttcggccaa
     4021 ctgggtcttt ccggcctcgg ccggtccaag caggcagacg ttgcggatgt ccgcagtagt
     4081 gtaagccatg tggtcttccc ccctacctca agctcacctt cgaagcttac ctcacggaaa
     4141 cggtcgggtc acacccgcca ggatcggggt tccactaccc tgggggccgg gaagagggtg
     4201 gcgcccgggc ctgtaacgat aaaggcccga gcgccgcgtg aagtcccgct aaaggagacg
     4261 agacgccggg ggaagagagg ggagagagtc aatgccagcg tctttcacgc tctactcttt
     4321 gcagagaccg tgccaacacc aaggaaacgg acaaaaccgc cagaaaacag ggggttga
//
LOCUS       DBNHQR010000060         2952 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1589279, whole genome shotgun sequence.
ACCESSION   DBNHQR010000060 DBNHQR010000000
VERSION     DBNHQR010000060.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1589279"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..1445)
                     /locus_tag="AC16VR_01320"
     CDS             complement(96..1445)
                     /locus_tag="AC16VR_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003299013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR6009820.1"
                     /translation="MPANEILALLMFVSFIALVFTGFPVAWILGGLAVLFTAFAIVLD
                     VDFNLAIGVDWNYASITVDRIWNVMENWVMVALPMFIFMGLLLDRSGIANQLMTSFAR
                     LFGGLRGGLAITVTLIGLLLAATTGIIGASVVLLALLGMPAMLKQGYDRALAAGTVCA
                     VGTLGILIPPSIMLVLMADRMAMSVGDLFLGAVFPGVLLGGLYILYLVGYGMIRPEAA
                     PAAENEPIDMAAVWGLIRAILPPAFLILAVLGSIFFGLATPTEAAGVGAAGALLLAWI
                     NRRLGKGVMTNVLQETTRTTAFIFAVLLGATAFSLVLRGLGGDELIEDALLSLPFEAT
                     GILICILLATFILGFFLDWIELTLIILPLVSPVVTGLGFDPVWFTILFAVCLQTSFLT
                     PPVGFAIFYLKGVAPEGVDVRTIYRGVVPFIVLQLIGLLIIFNWKALVTWLPAQAYS"
     gene            complement(1435..1974)
                     /locus_tag="AC16VR_01325"
     CDS             complement(1435..1974)
                     /locus_tag="AC16VR_01325"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HIR6009821.1"
                     /translation="MLPETTFSRWADAWLVRIGQWASWLWLLLLIVIVTNVTLRYAFG
                     QGRIEFEEIQWHIYAAGFLLGLAYAYQADAHIRVDVLHERFSPRLQAWLELYGIILFL
                     LPFIALILIYAVPFVAISFTQSEVSLAPGGLPMRWLMKAMLPVGFGLLLLAVLSRLSR
                     VWAFLFFSDPDSDSEHHAG"
     gene            complement(1975..>2952)
                     /locus_tag="AC16VR_01330"
     CDS             complement(1975..>2952)
                     /locus_tag="AC16VR_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014578924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR6009822.1"
                     /translation="LRIHWRMPVAFATNLPVLGDNALYVTGAIRAASNGALRIDPYEP
                     NEIVPPFSITDAVRDRKVEAGYSWLGYDQGRIPASPLIGAVPFGMEPWEFSAWWFEAG
                     GRELGEALYAKQNTHILFCGISGPETAGWFRERIDSLEQMKGLKIRFAGLGGKVIEQI
                     GASVTMLPGGEIFQALEKGAIDATEYSLPIVDEKLGFNRIARYNYYPGWHQPSTAVHL
                     IVNLDIWNALAYHDQQLIEMACTAGVTRNLARSEGLQGAVMARFPELGVSAETLPEEV
                     LRELQRVSEAVMLEQAEKDADFKKILESQQAFRSDYAIWKSRAYLPRNF"
BASE COUNT          608 a          907 c          970 g          467 t
ORIGIN      
        1 agaagacctg ccggtgcagg ccgtgccaca tgtccatcgg gacctgcaca gcatggcgga
       61 tcttcaggcg ttcgcagaga ccgttcggaa ctgactcagc tgtaggcctg ggccggaagc
      121 caggtaacga gcgccttcca gttgaagatg atcagcaggc cgatcagctg aagaacgatg
      181 aacggcacca cgccccggta gatggtccga acgtccactc cctccggtgc cacgcctttc
      241 aggtagaaga tcgcaaagcc taccggcggg gttagaaagc tggtctgcag gcagacggcg
      301 aacaggatcg tgaaccaaac cggatcgaac cccagccccg tgaccacggg cgaaaccagc
      361 ggcaggatga tcagcgtgag ctcgatccag tcgagaaaga acccgagtat gaaggttgcc
      421 agcagtatgc agatcaggat gcccgtggct tcgaagggca ggctgagcag ggcgtcttcg
      481 atgagctcat ctccgccgag acctcgcagg accagcgaga aagcagtggc gccgagcagc
      541 acggcgaaga tgaaggccgt cgttcgcgtg gtttcctgca gcacgttggt catcaccccc
      601 ttgcccagcc gacgattgat ccacgcgagc agcaaggccc cggccgcgcc cacgcccgcc
      661 gcctccgttg gcgttgcgag cccgaagaag atgctgccga gcacggccag gatcagaaag
      721 gccggcggca gaatcgccct gatgagaccc cacacggcgg ccatgtcgat cggttcgttt
      781 tccgcggcgg gggccgcttc ggggcggatc atgccgtagc cgacgaggta gagaatgtag
      841 aggccgccca gcagaacgcc gggaaagacg gcgcccagaa acaggtcgcc gacgctcatg
      901 gccatgcggt cggccataag caccagcatg atgctaggcg gtatcagtat tcccagggtg
      961 cccaccgcgc agacggtgcc ggcggcgagc gcgcgatcgt aaccctgctt gagcatagcg
     1021 ggcatcccga gcagggccag cagcaccacg gaggccccga tgatgcccgt ggtcgccgcg
     1081 agcagaagcc cgatcagcgt caccgtgatc gcaagtcctc cgcgcagccc gccgaacagt
     1141 cgtgcaaagc tggtcatgag ctggttggca atgccggacc ggtccagcag caggcccatg
     1201 aagatgaaca tgggcaaggc gaccatcacc cagttctcca tcacgttcca gatccggtcc
     1261 acggtgatgg atgcgtagtt ccagtccacg ccgatggcga ggttgaagtc cacatccagc
     1321 acgatggcga atgcggtgaa gaggacggcg agccctccga gtatccatgc cacggggaag
     1381 ccggtgaaga ccagggcgat gaagctcacg aacatgagca gggcgagaat ctcgttagcc
     1441 ggcatgatgt tcgctgtccg agtccgggtc gctgaagaag agaaaggccc acactcgact
     1501 cagccgcgag agcacggcca ggagcagcag cccgaacccg acgggaagca tcgccttcat
     1561 cagccaccgc atcggcaggc cgccgggcgc cagggatacc tcgctctgcg tgaagctgat
     1621 ggctacgaag gggaccgcgt agatcaggat gagggcgatg aacggcagca ggaacaggat
     1681 gatgccgtac agctccagcc aggcctgaag tctcggggag aaccgttcgt gcaggacgtc
     1741 gacgcgaatg tgggcgtccg cctggtaggc gtacgcaagc cccaggagga atccggcggc
     1801 gtagatgtgc cactggatct cttcgaactc gatgcgcccc tggccgaagg cgtaacgcag
     1861 ggtcacgttt gtgacgatga cgatcagcag cagcagccac agccaggaag cccattggcc
     1921 gatgcgaacg agccaggcat ccgcccaccg tgagaacgtg gtttcaggca gcactcagaa
     1981 gttccttggc aggtacgcgc gggacttcca gatggcgtag tcggagcgga atgcctgttg
     2041 cgactcgagt atctttttga aatccgcgtc cttctcggcc tgctcgagca tgaccgcttc
     2101 gctcaccctt tgaagttccc gcagcacctc ttcgggaagc gtttccgcgg agacgccgag
     2161 ttctggaaat ctggccatga cggccccctg caacccctcg gagcgggcga ggttgcgggt
     2221 gactcccgcc gtgcaagcca tctcgatcag ctgctggtcg tggtaggcca gggcgttcca
     2281 gatatccagg ttgacgatca gatgcacggc cgtcgacggc tggtgccagc cgggatagta
     2341 gttgtagcgc gcgatccggt tgaagccgag cttctcgtcg acgatgggca gcgagtattc
     2401 ggttgcgtcg atggcgccct tctctagcgc ctgaaagatc tcgccgccgg gcagcatcgt
     2461 tacggacgcg ccgatctgct cgatgacttt gccgccgaga cccgcaaagc gaatcttcag
     2521 gcccttcatc tgctcgaggg aatcgatgcg ctcgcggaac caacccgccg tttccggccc
     2581 gctgatcccg cagaaaagga tgtgggtgtt ctgcttggcg tacagcgctt cgccgagctc
     2641 ccgcccgccg gcctcgaacc accaggcgga gaactcccag ggctccatgc cgaaggggac
     2701 agcgccgatg agcggtgacg cgggaatgcg tccctggtcg taacccagcc aggagtagcc
     2761 agcctccact tttcggtccc gcacggcatc cgtgatgctg aagggcggca cgatctcgtt
     2821 gggctcgtag gggtcgatgc gaagggcacc gttggaagcc gcgcgaatgg cgcccgtgac
     2881 gtacagggcg ttgtcgccga gcacgggcag gttggtcgcg aaggcgacgg gcatgcgcca
     2941 gtggatgcgc ag
//
LOCUS       DBNHQR010000061         2125 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1082545, whole genome shotgun sequence.
ACCESSION   DBNHQR010000061 DBNHQR010000000
VERSION     DBNHQR010000061.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1082545"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2125)
                     /locus_tag="AC16VR_01335"
     CDS             complement(<1..>2125)
                     /locus_tag="AC16VR_01335"
                     /inference="COORDINATES: protein motif:HMM:NF020083.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HIR6009823.1"
                     /translation="DQAGPIAYAHHGSLAREIRTEVEERLKAGSLKAIVATSSLEMGI
                     DIGHLDEVVLVQSPPSIAATLQRIGRAGHGVGETSVGTLYPTHANDFLEAIVLAKAVA
                     GGDIEPLTPQENALDVLAQIIVSMCASESWQLDDVFALLRRSTPYHALPREQFDLLID
                     MLAGRYAGSRVRELKPRIVLDRINNTIRAHKGAVLALYNSGGTIPDRGYYQLRHADTG
                     AVIGELDEEFVWEATTGQIFSMGTQNWQVTRVTHNDVLVRSAPPGTTAPPFWRSESYN
                     RSFHFSERIARELEQAEALLDDGRNDDLRSTFTSAGGFEPEAAVELLDYLTRQREASE
                     APLPHRHHLLLELVRSGPGGYRGPDDTRQLVLHTFWGGRLNRPWALALEAAWQDALGR
                     RAEIHADNNAIVIQVKGEPDPARVLAMVTADNLDELLRRSLERSGFFGARFRECAGRA
                     LLLPRQRFNQRLPLWMSRLQAKKLMSATAGYPDFPMLLESWRTCLKDEFELDSLRQML
                     TELEDGTIEWTFVSTSSPSPFAANVTFAQINRYMYADDTPEEDAGPSALSDDLIRSAV
                     RNAGLRPPIRREIIQEYLAKRQRTAEGYRPVSAEDWSEWVKERVLIPAAEVRAATSAD
                     APVPEHEHLVWLEERAGSGENRAWLTHRELLHGLAQRGLRDGLAFQGTVPDVEESRSA
                     EQLALEILSFYGPLTESDIAWILPRV"
BASE COUNT          328 a          690 c          739 g          368 t
ORIGIN      
        1 aacccggggc agaatccagg cgatgtcgga ctccgtcaag gggccgtaga aagacaggat
       61 ctccagggcg agctgctcgg cacttcggct ctcctcgacg tccgggaccg tcccttggaa
      121 ggcaaggccg tcccgcagac cccgttgggc gagcccgtgg agcaactccc gatgggtgag
      181 ccaggcgcgg ttctctccgg agccggcgcg ctcctccagc cagacgaggt gctcgtgctc
      241 gggtaccggc gcgtccgcac tggtggcggc ccggacctcg gcggccggaa tcagaacccg
      301 ctccttgacc cattcggacc agtcctcggc gctcaccggg cggtatcctt cggccgtgcg
      361 ctgccgtttc gccaggtact cctgaatgat ttcgcgccgg atgggcgggc gcaggccggc
      421 gttgcgcacg gcgctgcgga tcagatcgtc ggacagcgcg ctgggcccgg cgtcttcttc
      481 cggggtgtcg tcggcgtaca tgtagcggtt gatctgggcg aaggtgacgt tggccgcgaa
      541 ggggctgggc gagctggtgg acacgaacgt ccactcgatg gtgccgtcct ccagttcggt
      601 cagcatctgt cgaagcgagt cgagttcgaa ctcgtccttc aggcaggtgc gccagctttc
      661 gagaagcatg gggaagtcgg ggtagccggc cgtcgcggac atcagcttct ttgcctgcag
      721 ccggctcatc cacagcggca gccgctggtt gaagcgttgc ctcggcaaga gcagggcccg
      781 gccggcgcac tctcgaaaac gcgcgccgaa gaatccggac cgctccaggg acctgcgcag
      841 cagctcgtcg aggttgtcgg ccgtcaccat ggcgagcacc cgcgcgggat cgggttcgcc
      901 ctttacctga atgacgatgg cgttgttgtc ggcgtgaatt tccgcgcgcc tgccgagcgc
      961 gtcctgccag gcggcctcca atgccagcgc ccacggccgg ttcagccggc cgccccagaa
     1021 cgtatgcagc accagctggc gggtatcgtc cggtccgcga taaccgcccg gtccgctgcg
     1081 gacgagttcc agcagcagat gatgccgatg gggcaggggg gcttcgctcg cctcccgctg
     1141 ccgggtcagg taatcgagca gctcgacggc cgcctcgggc tcgaagccgc ccgcgctggt
     1201 gaacgtggat cgaagatcgt cgttgcgccc gtcgtcgagc aaggcttcgg cttgctcgag
     1261 ctcgcgggcg atgcgctccg agaagtggaa gctccggttg tacgactccg agcgccagaa
     1321 cggcggagcc gtcgtgcccg gtggtgccga gcgaacgagc acatcgttgt gcgtcacccg
     1381 cgtgacctgc cagttctggg tgcccatgct gaagatctga cccgtggtgg cctcccagac
     1441 gaactcctcg tcgagctcgc cgatcaccgc accggtgtcg gcgtggcgaa gctggtaata
     1501 gccgcggtcg gggatggtgc cgccggagtt gtacagggcg aggaccgcgc ccttatgcgc
     1561 tcgaatcgtg ttgtttatcc ggtccagaac gatgcgcggt ttcagctccc gcacccgtga
     1621 gcctgcatag cgcccggcca gcatgtcgat cagcagatcg aactgctccc gcggcagcgc
     1681 atggtacggg gtgctgcggc gaagcagagc gaagacatcg tcgagctgcc agctttcgct
     1741 ggcgcacatg gacacgatga tctgggcgag cacgtccagc gcgttctcct gaggagtcag
     1801 cggctcgatg tcgcccccgg ctaccgcctt ggccaggacg atggcctcga gaaagtcgtt
     1861 tgcgtgggta gggtagagcg tgccgacgct ggtctctccc acgccgtgac cggcccgacc
     1921 gatgcgctga agcgtggccg cgatggaagg cggagactgg accaggacga cctcgtcgag
     1981 atggccgatg tcgataccca tctccagaga gctggtcgcg acgatggcct tgaggctgcc
     2041 ggccttcagc cgctcttcca cctccgtgcg gatctctctg gccagcgagc cgtggtgagc
     2101 gtaggcgatg ggcccggcct ggtcc
//
LOCUS       DBNHQR010000062         2449 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1082841, whole genome shotgun sequence.
ACCESSION   DBNHQR010000062 DBNHQR010000000
VERSION     DBNHQR010000062.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1082841"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="AC16VR_01340"
     CDS             complement(<1..189)
                     /locus_tag="AC16VR_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009824.1"
                     /translation="MIEGHLNAFHLGSLDRIGIQASQHIAPFDQVALVDQKLGHDGNL
                     GRPDELKDAVVGLYPAQAR"
     gene            100..333
                     /locus_tag="AC16VR_01345"
                     /pseudo
     CDS             100..333
                     /locus_tag="AC16VR_01345"
                     /inference="COORDINATES: protein motif:HMM:NF052757.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(241..492)
                     /locus_tag="AC16VR_01350"
     CDS             complement(241..492)
                     /locus_tag="AC16VR_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009825.1"
                     /translation="MLSEADLRASQPADDGEQRALAGTGRSGQHHQLADALAFTRMDG
                     QDLAVYRSFDPRPGELGARGDQFGLVDLDVPLRFVERRF"
     gene            575..856
                     /locus_tag="AC16VR_01355"
     CDS             575..856
                     /locus_tag="AC16VR_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009826.1"
                     /translation="MLADEPTASLDRESSEQVMSIVELLRQRQIFSGAELDLVVDMAI
                     AQAQAGNQTFQSEKDREALNQAVRAVAMADNVVTEEELAVIDELTKRQP"
     gene            complement(890..1570)
                     /locus_tag="AC16VR_01360"
     CDS             complement(890..1570)
                     /locus_tag="AC16VR_01360"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6009827.1"
                     /translation="MVPEYPAALIFDCDGVLVDSEIVAQRIERAHLASIGLHYEQVEF
                     VHRFTGTSEPEFLRRVEDDALERLRRKLPDGIIDEMKRAVQAELERVLQPISGVRAML
                     ACWDGPKAVASSSTLAALRFKLGRTGLDTAFGAHVYSAELVGRGKPDPAVFLHAAARL
                     GLDPGRCAVIEDSVNGILGAKRAGMTAIGFIGGAHCVPGHADALREVGAQWTFSNFAE
                     LSEFLDLI"
     gene            1819..2226
                     /locus_tag="AC16VR_01365"
     CDS             1819..2226
                     /locus_tag="AC16VR_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009828.1"
                     /translation="MLKVGRERSARQITALLLVTASIIGPAARAEPWAFTGRVGTAQG
                     DQAYFGSTGLGLSAFTGNVRLPNGRFIERDRFGLDAQWYRGPFGVMTEVSVGDDDGAD
                     VVNVLTELNLISRRERFAAYVQERWFNKKPNES"
BASE COUNT          464 a          780 c          792 g          413 t
ORIGIN      
        1 tcgagcctgg gccggataga gccccacgac ggcgtctttc agctcgtcgg gccttccgag
       61 attgccgtcg tggccgagct tctggtcgac gagggcgacc tggtcaaacg gggcgatgtg
      121 ctggctcgcc tggataccta tgcgatcaag gctgccgagg tgaaacgcgt tcaggtggcc
      181 ctcgatcatg ccaagcgggt gctgaagcgc cagcaggagc tcaagaagag ctcgttccag
      241 tcagaagcgg cgctcgacga agcgcagagg gacgtcgaga tctaccaggc cgaactgatc
      301 gccgcgcgcg cccagctcgc ccgggcgagg atcaaagctc cggtagacgg ccaggtcctg
      361 gccgtccatg cgcgtgaagg cgagcgcatc ggcaagctgg tggtgctgac cggaccttcc
      421 ggttccggca agagcacgct gctcaccatc atcggcgggc tgcgacgcgc gaaggtcggc
      481 ctcggagagc atacccacaa gcttcccgga cagctctccg gcgggcaaca gcagcgagcg
      541 gggatggcgc gcgctggtaa accagcctcg gctcgtgctg gcggacgaac cgacggcctc
      601 tctggaccgg gaatccagcg agcaggtcat gtccatcgtc gagttgctgc gccagcgcca
      661 gattttttcc ggggcggagc tcgacctggt ggtggacatg gcaatcgccc aggctcaggc
      721 agggaaccag acgttccaga gcgagaaaga ccgcgaagcg ctgaaccagg cggtgagagc
      781 cgttgccatg gccgacaacg tggtaacgga ggaggaactt gccgtcatcg acgagctgac
      841 caaacggcag ccttgatccg gggcgcagcc cagccagagt cagcctctct cagatcaaat
      901 ccaggaattc actgagctcg gcaaagttgc tgaatgtcca ctgtgcgccg acctcgcgca
      961 gcgcgtccgc atgaccgggc acgcagtgtg ctccgccgat gaatccgatg gctgtcattc
     1021 ccgcccgttt cgcccccagg atgccgttca cgctgtcctc gatcaccgcg caacgcccag
     1081 ggtccaaccc aagacgggcc gccgcgtgca ggaacacggc gggatcaggc ttgccacggc
     1141 cgacgagctc ggcagagtag acgtgagcac cgaaggccgt gtcgagcccg gtacgaccca
     1201 gcttgaagcg cagagccgct aaggtgctcg atgaagccac cgctttcggg ccgtcccaac
     1261 aggctagcat cgcgcggacg cccgagatcg gttggagcac gcgttcaagc tccgcctgca
     1321 cggcgcgctt catctcgtca atgatcccat ccggaagctt cctgcgcagc cgctccagag
     1381 cgtcgtcttc cacccgccgg aggaactccg gctcggaggt gccggtaaag cgatgaacga
     1441 actcgacctg ctcgtagtga aggccgatcg aggccaggtg cgcacgctcg attcgctggg
     1501 cgacgatttc gctgtccacc agcaccccat cgcagtcgaa gatgagcgcc gccgggtact
     1561 cagggaccaa ggggaacgct cctgtggatc acctggcacg aaccgtgtag accatccggc
     1621 ggcggcgtca ttgatcgctg cctgagtctg tttggcttga gattcgcatt ttgcgacgca
     1681 tttcgcgaat tctgttgcat tgcgcggcgc tcgatggcgc tgagagccct gattatccgg
     1741 cgatccgtca gctggcgcac caattgcatt cattgccata gaagttgcgc tcgacggttg
     1801 cggcggacgg agacaaccat gctaaaggtc ggacgggaac gctcggctcg ccagatcacg
     1861 gcgctcctgc tggtcacggc atcgatcatc gggcccgcag cccgggcaga gccctgggca
     1921 ttcaccggca gggtcggcac cgcgcagggc gaccaggcgt acttcggctc cacagggttg
     1981 ggcctgtcgg cgttcaccgg caatgtccgc ctgccgaatg gtcgtttcat cgagcgcgac
     2041 cgcttcgggc tggatgcgca atggtaccgg gggccgttcg gcgtgatgac cgaagtctcg
     2101 gtcggcgatg acgacggtgc cgacgtcgtg aacgtgctca ccgaattgaa cctgatctcg
     2161 aggcgcgagc gtttcgccgc ctatgttcag gaacgctggt tcaacaagaa gcccaacgaa
     2221 agctgaaacg atcagacctc ctgggcggca ggtgcccgct acctggcttc gcgcaatctc
     2281 gaggtcagct tccagtacct caacgagcag gaggtctacg gaccccgcga cgaggagtcc
     2341 gttgggcagc ttcaattcag gtacaggaca cttcaatgac gctttcccgc aaggacggtc
     2401 ctctggcaag tctcacactt ctggccgcgc tgctcgcagc cggcggacc
//
LOCUS       DBNHQR010000063         2287 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_956304, whole genome shotgun sequence.
ACCESSION   DBNHQR010000063 DBNHQR010000000
VERSION     DBNHQR010000063.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_956304"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="AC16VR_01370"
     CDS             complement(<1..310)
                     /locus_tag="AC16VR_01370"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HIR6009829.1"
                     /translation="MQFKNRVTEMLGVEIPVVQAPMGWIARSQLASAVSNAGAMGIIE
                     TSSGELDAVREEIRRMRELTDKPFGVNIAQAFVRDPNIVQFVIDQGVKFVTTSAGNPE
                     R"
     gene            517..1803
                     /locus_tag="AC16VR_01375"
     CDS             517..1803
                     /locus_tag="AC16VR_01375"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6009830.1"
                     /translation="MQARPPKPFKARDLTADRATAMRFTAVLGLQMGQIFPGSFIALF
                     LPVIYREQGLSLEMYWVFTLPAIPTWIRPLWAPFVDRTGSRIIGMRKTWFIPCTVFGA
                     LAYLSLTQFAPTLENLAVIITILVIKTTFMTTQDIAIDGYMVENIRDEERPVGAAVLD
                     IGRNVARFIAWAGIAWVYTEHGWSAAVTVASALLILFSAPGIIRREPPRPSQFDHVRP
                     SLKNLLRRRDSYYVYPLCFGIALMGALVQSLYPTFLSDLGFSLGEVAAVIAPAILIGS
                     VLGASVTSWYLGRFGYKATVLTAAFAVVLAMLPIMWMGSLEEPTFVVVFAVTLNAIAL
                     PSFLDVSFQAARLKWASKSQAATDYTSQIVTMAAGGGLAVAVGGFMAEYLGWFYYFLV
                     AGTLVSGACFVLYYLFDTIEDIVEERDRTEAEGGNL"
     gene            1863..>2287
                     /locus_tag="AC16VR_01380"
     CDS             1863..>2287
                     /locus_tag="AC16VR_01380"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6009831.1"
                     /translation="MALVDYEIHGHIAVITLNRPEARNAINPEVAVRLADAWESVRDD
                     DQVRVAILTGTGSAFCAGADLGQLIPMVSGTRKPENEWDERVASEPGITNRALLRNFD
                     TEKPIIAAINGHAIAGGMEIVQGTDIRVSVAEAKFGVQEV"
BASE COUNT          406 a          729 c          729 g          423 t
ORIGIN      
        1 accgctccgg attgccggca gacgtcgtca cgaacttgac gccctggtcg atgacgaact
       61 gaacgatgtt ggggtcgcgt acgaacgcct gggcgatgtt gacaccgaac ggcttgtcgg
      121 tgagctcgcg catcctgcgg atttcctccc gaacggcgtc gagctccccg gaggacgttt
      181 caatgatgcc catggcgccc gcgttcgaca cggcggaggc gagctgcgag cgggcaatcc
      241 atcccatcgg agcctgcacg acgggtatct cgacccccag catctcggtt actcggttct
      301 tgaactgcat gacgcccccc ggggaaatcg gggagcctac ctcagctgcc ggtggacgtg
      361 tagtggcgca ggccgaatcg tcagaggaag acggccagcc cgaaatagag gaacccagca
      421 gggtgcgggt ggccggaaag ggccgcggat tctactggag cgtcaatcgc agaaacaacc
      481 cgtgtgccat ccgggcgccg ccgctaaagt ggccggatgc aggcccgccc acccaagccg
      541 ttcaaagcgc gcgacctgac cgccgatcgg gcgacggcca tgcgcttcac cgcggtgctc
      601 ggccttcaga tgggtcagat ctttccgggc tcgttcatcg ccctgtttct gccggtgatc
      661 taccgcgagc aggggctgtc gctggagatg tactgggtct tcacgctccc cgccatcccg
      721 acctggatac ggccgctctg ggcgccgttc gtcgaccgga cgggcagccg catcatcggc
      781 atgcgaaaaa cctggttcat tccctgcacg gtcttcggcg ctcttgcgta tctcagcctg
      841 acccaattcg cgccaaccct cgaaaatctc gccgtgatca tcacgattct ggtgatcaag
      901 accacgttca tgaccaccca ggacatcgcc atagacggct acatggtcga gaacatccgg
      961 gacgaggagc ggccggtggg cgccgccgtg ctggacatcg gccgcaacgt ggccaggttc
     1021 atcgcgtggg ccggcatcgc ctgggtctac acggagcatg gctggtcggc cgcggtcacc
     1081 gtggcctcgg ctctgctgat cctgttctcc gcaccaggaa tcattcgcag ggaaccgccg
     1141 aggccgtccc agttcgacca tgtccggccg agcctcaaga acctgcttcg ccgtcgcgac
     1201 agctactacg tctaccctct ctgctttgga atcgctctga tgggcgcgct ggtgcagtcg
     1261 ctgtacccaa cgtttctttc cgatctgggc ttcagcctcg gcgaggttgc cgccgtgatc
     1321 gccccggcca tcctgatcgg ttcggttctg ggggcgtcgg tcaccagctg gtatctggga
     1381 cgattcgggt acaaggcaac ggttctcacg gcggcgttcg cggtggttct ggcgatgctg
     1441 cccatcatgt ggatgggatc gctggaggaa ccgacattcg tcgtggtctt cgcggtgacc
     1501 ctcaacgcca ttgctctgcc ttcgttcctc gatgtgagct ttcaggctgc gcgcctgaag
     1561 tgggcgtcca aatctcaagc ggccaccgac tacacctcgc agatcgtcac catggcggcg
     1621 ggcggggggc tggcggtcgc cgtgggcggt ttcatggccg agtacctggg atggttctac
     1681 tacttcctgg tcgcgggaac cctggtgagc ggcgcctgct tcgtgctcta ctatctcttc
     1741 gacacgatcg aagacatcgt cgaggagcgc gaccggacgg aagccgaggg cggtaacctc
     1801 tgacccttca cgagcagagc caagcccgcg tcacaacaaa tgcagaggaa ggggaggatc
     1861 caatggcact cgtagactac gaaattcacg gacacatcgc ggttatcact ttgaaccggc
     1921 cggaagcccg caacgcgatc aatccggagg tggccgtacg cctggcagat gcctgggaat
     1981 cggtacgtga cgacgaccag gtccgcgtcg ccatcctgac gggcacgggc agcgcgttct
     2041 gcgccggggc cgatctgggc cagctcatcc caatggtctc cggaacgcgc aagcccgaga
     2101 acgagtggga cgagcgtgtg gcttccgagc cgggcatcac caaccgggcg ctgctgcgca
     2161 acttcgatac cgagaagccg atcattgcgg caatcaacgg ccacgccatc gcaggtggca
     2221 tggagatcgt ccagggcacg gacatccgcg tctccgtggc ggaggcgaag ttcggcgttc
     2281 aggaggt
//
LOCUS       DBNHQR010000064         5874 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_68978, whole genome shotgun sequence.
ACCESSION   DBNHQR010000064 DBNHQR010000000
VERSION     DBNHQR010000064.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5874)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_68978"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="AC16VR_01385"
     CDS             complement(<1..918)
                     /locus_tag="AC16VR_01385"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF004224.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HIR6009832.1"
                     /translation="MSSGVNVAIAGATGAVGEALLEVLEQRKFPVARLSLLASERSEG
                     KRLQFRGKSVPVERLDNFDFSDTAIAFFSAGGDLSAQFAPKAAAAGCIVVDNTSQFRY
                     DADIPLVVPEVNAHRLDQPNPRNIIANPNCSTIQMVVALKPLHDVARIRRINVATYQA
                     VSGAGASAIRELAGQTASLLNGKPIESKVFPVQIAFNALPHIDKFQDNGYTKEEMKMV
                     WETQKILEDEAIRVNPTCVRVPVFFGHSEAVHIETEHKITAEEARRLLAAAPGVTLID
                     DPSANGYPTAVSHGAGQDAVFVGRVREDVS"
     gene            complement(941..2041)
                     /gene="leuB"
                     /locus_tag="AC16VR_01390"
     CDS             complement(941..2041)
                     /gene="leuB"
                     /locus_tag="AC16VR_01390"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003446163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HIR6009833.1"
                     /translation="MTENHKILVLPGDGIGAEIVPVAQAVMGAAALKFGVSVAFEEGL
                     VGGIAIDETGSPLPDETLRQAREARAVLLGAVGGPRWDDLPTLQRPERGLLGLRAGLE
                     LFANLRPAIQFGPLAEASSLRAELVAGLDILIIRELTGGIYFGEPRGIEVVDNERRGF
                     NTYVYSEHEIERIARVAFELARKRDRRVCSVDKANVLEVTQLWREVVSEVAGDYPDVE
                     LSHMYVDNAGMQLVRAPKQFDVVVTGNMFGDILSDVAAMLTGSLGMLPSASLSSDGRG
                     LYEPVHGTAPDIAGQDKANPLATILSVAMLFRYSLDAPEAARAIESAVDEVLAAGWRT
                     ADILQAEDSSKLRLIGTREMGDRVLEQLETGA"
     gene            complement(2061..2711)
                     /gene="leuD"
                     /locus_tag="AC16VR_01395"
     CDS             complement(2061..2711)
                     /gene="leuD"
                     /locus_tag="AC16VR_01395"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010794947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HIR6009834.1"
                     /translation="MKAFDKVTGIVVPLDRANVDTDQIIPKQFLKSIHRTGFGDNLFD
                     AWRFEDEGDIGITPNERRINRAFVLNDPRYQGGSILLARANFGCGSSREHAVWALLEY
                     GFRCVIAPSFADIFLSNCFKNGLLPIVLEKEIIEELFVRSAGEEPLELTVDLPGQEVI
                     AGMGERYEFAIDANRKRALVEGLDDIGMTLANVDAIRAFEERHRRAMPWLFESHPT"
     gene            complement(2708..4123)
                     /gene="leuC"
                     /locus_tag="AC16VR_01400"
     CDS             complement(2708..4123)
                     /gene="leuC"
                     /locus_tag="AC16VR_01400"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIR6009835.1"
                     /translation="MAAKTLYDKIWDAHVVGVREDGQTMIYIDRHLLHEVTSPQAFEG
                     LALAGRKPWRLDANLATPDHNVPTDRRELGIEGITDLIAREQVETLDANCRRYGITQF
                     DITDRRQGIVHVVGPEQGATLPGMTIVCGDSHTSTHGAFAALAQGIGTSDVEHVLATQ
                     CLVQPKALNFRVAAEGELGVGVYAKDLVLAIIGRIGTAGATGHTIEYQGSAIRSLTME
                     GRMTLCNMSIEAGARAGLVAVDDRTLEYLRERPFAPVGAAWDAAEAYWRGLVTDSQAH
                     FDRDLELNASEIEPQVTWGTSPEMVCGISASTPNPDSVDDPQRSDGLRRALEYMGLGS
                     DQPMTEIAVDRVFIGSCTNSRIEDLRAAARVVKGRRVAGSLKQALVVPGSGLVKAQAE
                     AEGLADVFLAAGFEWRAPGCSMCLAMNPDRLLPGEHCASTSNRNFEGRQGHGGRTHLV
                     SPAMAAAAGIAGRFVDVREYL"
     gene            4227..5147
                     /locus_tag="AC16VR_01405"
     CDS             4227..5147
                     /locus_tag="AC16VR_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008056358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR6009836.1"
                     /translation="MNWNELRAFVSVARHASFSKAAEELHITQPAVTKRVQALEGSLS
                     ARLFDRVGKRVHLTDAGTRLLPRAEHLLREMSDTQRLLRNLKHEVSGVLSLATSHHVG
                     LHRLAPILREFTQRYPDVRLDIRFEDSEAAHDLVREAESELAVVTLDPLGDTELAYEH
                     LWHDPLSFVVAAEHALAGSSKLTLTELARHPVIMPGLGTYTGRIVAEVFSAAGVPLTP
                     AMSTNYLETIGMLVSTGLGWSVLPASMMSADLVSIRTEAPSLHRSLGLVTHPRRTLSN
                     AAQAFTAVALEHRDAISASAPAPDSGLGGA"
     gene            complement(5107..>5874)
                     /gene="aroC"
                     /locus_tag="AC16VR_01410"
     CDS             complement(5107..>5874)
                     /gene="aroC"
                     /locus_tag="AC16VR_01410"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008031492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIR6009837.1"
                     /translation="FRPAHADLTYQEKYGVRDYRGGGRASARETAMRVAAGAIAKKYL
                     KERLGLQVRGRLSQIGDVRVGFKSWDVVEDNPFFCADPEAVDAIAALIDGLRREGDSI
                     GAEVTVVATLVPVGLGEPVFGKLDAEIAGALMSINAVKAVGIGDGFDVVGQRGTAHRD
                     EMTAEGFLSNHAGGVLGGISSGQDIIARIALKPTSSLVTPGRSVDKAGNEVEVVTKGR
                     HDPCVGIRATPIAEAMVALVLMDHVMRHRAQNPERGH"
BASE COUNT         1050 a         1971 c         1785 g         1068 t
ORIGIN      
        1 ggagacgtcc tcgcgcaccc tccccacgaa cacggcatcc tggcccgcgc cgtggctcac
       61 cgccgtcggg tagccgttcg ccgagggatc atcgatcagc gtgacgccgg gtgctgcggc
      121 cagcagacgg cgggcttcct ccgccgtgat cttgtgctcg gtttcgatgt gcaccgcttc
      181 cgagtggccg aagaagacgg gcaccctgac acaggttgga ttcacccgaa tcgcttcgtc
      241 ctccaggatc ttctgggttt cccacaccat cttcatctct tctttggtgt agccgttgtc
      301 ctgaaacttg tcgatatgtg gcagcgcgtt gaatgcaatc tgcaccggga agaccttgct
      361 ctcgatgggc ttgccgttca gcagactggc cgtctgaccc gcaagctccc gtatggcgct
      421 cgccccagca ccggacaccg cctgatacgt cgcgacgttt atgcgacgga ttcgggccac
      481 gtcatgcagg ggcttgagcg ccaccaccat ctggatcgtc gagcagttcg ggttggcaat
      541 gatattgcgc ggatttggct ggtccagacg atgggcattt acctccggaa cgaccagcgg
      601 gatgtctgcg tcgtaccgga actgggaggt attgtcgacg acgatgcagc cggcagccgc
      661 cgccttcggc gcgaactgcg ccgagaggtc cccgccggcg gagaaaaaag cgatggccgt
      721 gtcggaaaaa tcgaagttgt ccagacgctc cacgggcacg gactttccgc gaaactgcag
      781 gcgctttcct tcggaccgct cgctggcaag cagcgaaagg cgggcgacgg gaaacttccg
      841 ctgctccagc acttcgagca gtgcctcgcc cacggcgccg gttgccccgg cgatggccac
      901 attgactcca ctgctcacgg catccgcctc caattgcctg tcacgccccg gtttcgagct
      961 gctcgagcac cctgtcaccc atctcccggg taccgatgag ccggagcttg gacgagtcct
     1021 cggcctgcag aatgtcggcc gtccgccacc cggccgcgag cacctcatcc acggcgctct
     1081 ctatggccct ggcggcttcc ggcgcgtcca ggctgtagcg gaagagcatc gccaccgaga
     1141 gaatggtggc caggggattt gccttgtcct gccccgcgat gtccggcgcc gtgccatgga
     1201 cgggctcgta gagccctctg ccgtcgctgc tcagcgacgc ggaaggcagc atgcccagcg
     1261 agccggtcag catggcggcc acgtctgaca ggatgtcgcc gaacatgttg cccgtcacca
     1321 ccacgtcgaa ctgcttcggc gcgcggacga gctgcatgcc ggcgttgtcc acgtacatgt
     1381 ggctgagctc cacgtccgga tagtcgccgg ccacctcgga tacgacctcc cgccagagct
     1441 gtgtgacctc gagcacgttc gccttgtcga ccgagcagac tctgcgatcc cgtttgcgcg
     1501 ccagctcaaa ggccacccgg gcgattcgtt cgatctcgtg ctcggagtag acgtaggtgt
     1561 tgaaaccacg acgctcgttg tccaccacct cgatgccccg cggctcgccg aaatagatgc
     1621 cgcctgtgag ctcccggatg atgagaatgt ccaggccggc aacgagctcg gcccgcagcg
     1681 acgacgcctc ggcgagaggg ccgaactgta tggccgggcg caggttggcg aaaagctcga
     1741 gcccggcgcg caggccgagc agcccccttt ccggccgctg caaggtaggc agatcgtccc
     1801 acctgggccc accgacagcc cccagcagca cggccctggc ctcgcgggcc tggcgcagcg
     1861 tctcgtcagg cagcggagaa ccggtctcgt cgatagcgat gcccccgacc agcccctcct
     1921 cgaaagcgac gctcacgccg aacttcaaag cggccgcgcc catcactgcc tgagcaaccg
     1981 ggacgatctc tgcgccgatc ccgtcgccgg gcagaacaag aattttatga ttttcagtca
     2041 cttgtgagat ctttgcaaaa tcaggttgga tgactctcga agagccaggg catggcccga
     2101 cgatggcgct cctcgaaggc ccgaatcgcg tccacgttgg ccagggtcat gccgatgtcg
     2161 tctagcccct ccaccaaggc gcgtttgcga ttcgcgtcga tggcaaactc gtatcgctcc
     2221 cccatcccgg cgatcacctc ctggcccggc aggtccacgg tgagctcaag cggctcttct
     2281 ccagcagagc gaacgaacaa ctcttcaata atttcttttt ctaaaacaat aggaagtaaa
     2341 ccatttttaa agcaattact tagaaaaata tcagcaaagc tgggggcgat cacgcagcgg
     2401 aacccgtact ccagcagcgc ccataccgcg tgctcacgac tcgaaccgca gccgaagttg
     2461 gcccgggcca gcaggattga gccaccctga tatcgggggt cgttgagcac gaaagcacgg
     2521 ttgatgcggc gctcgttcgg cgtgatgccg atgtcgccct cgtcttcgaa acgccaggcg
     2581 tcgaagaggt tatccccgaa gcccgtccgg tggatggatt tgaggaactg cttgggaatg
     2641 atctggtcgg tgtccacgtt ggcccggtcc agcggaacca cgatgcccgt taccttatcg
     2701 aacgccttca caggtactcc ctcacgtcaa cgaatctgcc ggcgatgccg gccgcggcgg
     2761 ccatggccgg gctcaccaga tgggtgcggc ctccgtgccc ctggcgcccc tcgaagttgc
     2821 gattcgaagt ggaagcgcag tgctccccgg gcagcagccg atccggattc atggcgagac
     2881 acatcgagca ccctggcgcg cgccactcga agccggcggc caggaagacg tcggccaaac
     2941 cttccgcttc ggcctgggct ttgacgagac cggaaccagg caccaccagc gcctgcttga
     3001 gactcccggc aacccgcctg cccttcacca cccgcgccgc cgcgcgcagg tcctcgatgc
     3061 gggagttggt gcaggagccg atgaaaaccc ggtcgacggc gatctctgtc atgggctgat
     3121 cactgccaag acccatgtat tcgagcgcgc ggcgcaaccc gtcggatcgt tgcgggtcgt
     3181 ccacgctgtc tggattcggg gtgctggcgg aaatgccgca caccatctcg ggcgaggttc
     3241 cccaggtcac ctggggctcg atctccgagg cgttcagctc cagatcccga tcgaaatgcg
     3301 cttgcgagtc ggttactagc cctcgccagt atgcctcggc cgcatcccag gccgccccaa
     3361 cgggcgcgaa cggtcgctcg cgcaggtatt ccagcgtgcg atcgtccacg gccaccaggc
     3421 ccgccctggc acccgcctcg atggacatgt tgcacagcgt catgcgccct tccatggtca
     3481 gggagcggat tgcggacccc tggtactcga tggtgtgtcc cgtcgctccc gcggtcccga
     3541 tgcggccgat gatggccaga acgaggtctt tggcatatac gccaacgcca agctcccctt
     3601 cagctgccac cctgaaatta agggccttgg gctggacgag gcactgggtc gccagaacgt
     3661 gctcaacatc ggaagtcccg atcccctgag ccagcgcggc aaaggcgcca tgggtggagg
     3721 tgtgggagtc tccgcaaacg atggtcattc ccggcagggt ggcgccctgc tccgggccca
     3781 ccacgtggac gataccctgg cgccggtcgg tgatgtcgaa ctgggtgatg ccgtagcgcc
     3841 ggcagttagc atccagggtc tcgacctgct ccctggcgat gaggtcggtg atgccctcga
     3901 tgcccagctc acgcctgtcg gtggggacgt tgtgatccgg ggtcgcgagg ttggcgtcca
     3961 atcgccaggg cttacggccc gcgagagcca gaccttcgaa agcctgaggc gaggtcactt
     4021 cgtgcagcag gtgacggtcg atgtagatca tggtctgacc gtcttcgcgc acgcccacca
     4081 catgagcgtc ccatatcttg tcgtaaaggg tttttgcggc cacgtcgcta cggctaaaaa
     4141 gtagatttgc acatcgtaga agccggctta gaataaaaca aattcataat ttttatactt
     4201 ttcataataa tctggaatga tttgtagtga actggaatga attgagggcc ttcgtttccg
     4261 tggcgcggca cgcgagcttc tccaaggcag cggaggagct gcacatcacc cagcccgccg
     4321 tcacgaaacg ggtgcaggcc ctggagggca gcctctcagc gaggctgttc gatcgcgttg
     4381 gcaagcgcgt tcacctcaca gacgccggca cccggcttct gcctagggcc gagcacctgc
     4441 tgcgggagat gagcgatact caacggctcc tgcgaaacct caagcacgaa gtctccggcg
     4501 tgctgagcct cgccacgtcg caccacgtgg gtctgcatcg gctcgccccc attctgcgcg
     4561 agtttaccca gcgctacccg gacgttcgcc tggacatccg cttcgaggac tcggaagccg
     4621 cccacgatct ggtgcgggag gcggaatccg agctggcggt ggtcacgctg gatcccttgg
     4681 gtgacaccga gctggcttac gagcatttat ggcacgaccc gctgagcttc gtggtcgccg
     4741 ccgagcacgc gctggccggc tcgagcaagc tcacgcttac cgagttggcg cgccatccgg
     4801 tgatcatgcc cggcctggga acgtacacgg ggcgaatcgt cgcagaggta ttctcagcgg
     4861 ccggggtgcc cctgacgccg gccatgtcga caaactatct cgaaaccatc gggatgctgg
     4921 tcagcaccgg cctgggctgg agcgtgctgc ctgcaagcat gatgagcgca gatctggtct
     4981 ccatccgtac cgaggcgccg tcgctgcatc ggtcgctggg cctggtaacg catccgcgac
     5041 gcacactatc caacgcggct caggcattta ccgcggtcgc cctggagcac cgcgatgcga
     5101 tttccgctag tgcccccgct ccggattctg ggctcggtgg cgcatgacgt ggtccatcag
     5161 caccagggca accatggctt ccgcaatggg cgtggcgcgt atcccgacgc acgggtcgtg
     5221 acgccctttg gttaccacct ccacctcgtt gccggctttg tccactgacc gtccgggggt
     5281 gaccaggctg gacgtcggct tgagcgcgat ccgcgcgatg atgtcctgac ccgaggagat
     5341 accgcccagc acgcctccgg cgtggttgct caggaaaccc tcggccgtca tctcgtcgcg
     5401 atgcgccgtg cctcgctggc caaccacatc gaagccgtcg cctatcccca ccgccttgac
     5461 cgcgttgatg ctcatcagcg cccccgcgat ctcggcatcg agcttaccga aaaccggctc
     5521 gccgaggccc accggcacca aggtcgccac gaccgtcacc tccgcaccga tggaatcacc
     5581 ctccctgcga aggccatcga tgagcgcggc gatggcatcg acggcttccg gatcagcgca
     5641 gaagaaagga ttgtcctcca ccacgtccca gcttttgaag ccgacccgaa cgtcaccgat
     5701 ctgcgagagc cgaccgcgca cctgcagccc gagccgttcc ttgagatact tcttcgcgat
     5761 ggcgcccgcc gcgacgcgca tggccgtctc ccgggccgat gcccggccgc caccccgata
     5821 gtcgcgaacg ccgtactttt cctggtaggt caaatccgcg tgggccggac ggaa
//
LOCUS       DBNHQR010000065         2595 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1209722, whole genome shotgun sequence.
ACCESSION   DBNHQR010000065 DBNHQR010000000
VERSION     DBNHQR010000065.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1209722"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            402..794
                     /locus_tag="AC16VR_01415"
     CDS             402..794
                     /locus_tag="AC16VR_01415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03757 family integrating conjugative element
                     protein"
                     /protein_id="HIR6009838.1"
                     /translation="MPAWAGDALVIEVFTTSEQPVVGGEHERLRIATNTIYAVDGLER
                     FESGLSEGLPADPNTAKAEALRRFQHLDDTSMAPAKNAAFGLAKAVQYGVDRYPAIVF
                     EGRAVVYGVTDLVEALNRYEAWQREQTR"
     gene            791..1819
                     /locus_tag="AC16VR_01420"
     CDS             791..1819
                     /locus_tag="AC16VR_01420"
                     /inference="COORDINATES: protein motif:HMM:TIGR03756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HIR6009839.1"
                     /translation="MRRQRLPVLGLTLLLLVSVGIQAATVTTPGIIAQTTAATFACMR
                     WMPVGVCFWLRCSWTGCRVRTSLKVGHYNPDLVVSVYNELGGNPWAEIRATLGLAQRA
                     AATGLLGALLPVPIDSAGNRTEGTGDRKDHRNLVFRESDAIGHPLSTLSGLVAGVGLV
                     CESQTTSFFPYFQSGLDALSWRQEIPEIFYAASWVPGLREVGTFPLQTWGSVHPRTGW
                     TTQAEEPKAAAVTAQRAGDIVTRSGQPHVYVPLTGPSSGSQRVWPPGPLVEKDASTGT
                     WQMLAPRPDASCNVFGTNDLVGLSGWGGGRVDAAGDYAWNLWRPYQCCSRRGQWFLFD
                     INWISYPQ"
     gene            1816..>2595
                     /locus_tag="AC16VR_01425"
     CDS             1816..>2595
                     /locus_tag="AC16VR_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009840.1"
                     /translation="MTFPNHGSSKLAAFAMLVAALTTPAAHAAEGPTEDSLWYYEIGG
                     AEPVSAPANPSVVSVTLGGSAQLGLGYSCGKFDPVLAVTNTLNDIKSGVDDMMNAMTA
                     AASSAIAALPALILQRANPGLYDLFQNALLKAEETMQLATKSCEQMEAEIAQGKNPYA
                     DLITLSKGNDWKVQMGIGGNDAVTAKESVETSNGDNGVPWIGGQAGGAGQPVLRFTGD
                     IVQAGYNINMNRPITSTAPVPAASATRLSDVWPTPAAARTWV"
BASE COUNT          449 a          870 c          830 g          446 t
ORIGIN      
        1 gccgttcgac acccgcactt ccgggtgcaa gtcgaggtag aagaacttgc tgcccttctg
       61 gccgggcgga aggaaccgat actggaacca ttgagggacg tagtgattgc gtgtgaatcc
      121 aggcatagca atacatttgc agcattttgc tcaaggccgt caatagcccc gcggatcggt
      181 acatccggcg ttggtccgca gatctgcaga gttggtggag gcaattcaag gtagttcgat
      241 cgcttcgaaa actgcaacgg ggagtgcggc ggttttccct tcctcggctc tcgatccgcg
      301 cggacgatgg cacgcatcgt cccgcaatcc gagacagaga gatgctcgct ttatcccacg
      361 ccctggttgg actgacgttc tctgggcccc tggctttcag cgtgcccgct tgggcgggcg
      421 acgccctggt catcgaggtc ttcaccacaa gcgagcagcc ggtcgttggt ggtgagcacg
      481 agcgactgcg aatcgcgacc aacacgatct acgccgttga cggcctcgag cgcttcgaat
      541 cagggctgtc cgaaggcctc ccggctgacc cgaacaccgc gaaggcggaa gccctccgtc
      601 ggttccagca cctcgacgac actagcatgg cgccggcgaa gaacgcggcg tttgggcttg
      661 ccaaggcggt gcagtacggc gtcgatcgtt atcctgccat cgttttcgag ggacgcgccg
      721 tcgtctacgg cgtgacggac ctggtcgagg cgctcaaccg ttacgaagct tggcaacggg
      781 agcagacccg atgaggcgtc agcgcctccc tgttctcggc ctgacattgc tgctcctggt
      841 atccgttggg atccaagccg caaccgtcac gacacccggc atcatcgcgc agaccacggc
      901 ggcgaccttc gcctgcatgc gctggatgcc ggtaggggtg tgcttctggc tgcgttgctc
      961 ctggaccggc tgtcgcgtac gcacgtcgct caaggtcggg cattacaacc cggacctggt
     1021 agtcagtgtc tacaacgagc tcggtggcaa tccgtgggcg gagatccgcg caacgctcgg
     1081 tctcgcccag cgcgctgctg caaccggtct gctgggcgcg ctcctgccgg tcccgatcga
     1141 tagcgcgggt aatcgcaccg aggggaccgg tgaccggaag gaccaccgca atctcgtgtt
     1201 ccgcgagagc gatgctatcg gccatccgct gagtacattg tccggccttg tcgccggcgt
     1261 cggcctcgtc tgcgaatccc agacgacgtc cttcttcccg tacttccagt ccgggctcga
     1321 tgccctgtcc tggcgccagg agatcccgga gatcttctat gcggccagct gggtgccggg
     1381 cctgcgcgag gtcggcacct ttccactgca gacctggggc agcgtgcatc ctcgcaccgg
     1441 ctggacgacc caggcggagg aacccaaggc cgccgccgtc actgcccagc gcgctggcga
     1501 catcgtcact cgcagtggcc agccgcacgt ctacgtgccg cttaccggac cgagctccgg
     1561 cagccaacgc gtctggccgc cgggcccgct ggtcgagaag gacgccagca ccggcacctg
     1621 gcagatgcta gcgccgcgcc cggatgcgag ctgcaacgtg ttcggtacca acgacctggt
     1681 gggtctctcc ggctggggcg gtggccgggt cgatgcggcc ggcgactacg cgtggaacct
     1741 gtggcgaccc taccagtgct gcagccgccg cgggcagtgg tttctgttcg acatcaactg
     1801 gatctcgtac ccgcaatgac attcccgaac cacggaagct ccaagctcgc cgccttcgcg
     1861 atgctggttg cggcgttgac cacgcccgcg gcgcacgccg ccgaaggccc gaccgaagac
     1921 agcctctggt actacgagat cggcggcgcc gagccggttt cggcccctgc caacccgtca
     1981 gtggtatcgg tgaccctcgg cggttccgcc cagctcgggc tcggttacag ctgcggcaag
     2041 ttcgacccgg tactcgcagt caccaacacc ctgaacgaca tcaagtctgg cgtcgacgac
     2101 atgatgaacg ccatgacggc cgccgcgtcc tcagccatcg ccgcgctgcc ggcgctgatc
     2161 ctccaacggg caaaccccgg cctctacgac ctgttccaga acgcgctgct gaaggcggag
     2221 gagaccatgc agctcgcgac caagagctgc gagcagatgg aggccgagat cgcccagggt
     2281 aagaacccct acgcggatct catcactctc tccaagggca acgactggaa agtacagatg
     2341 gggatcggcg ggaacgacgc cgtgaccgcg aaggaatcgg ttgagacctc gaacggcgac
     2401 aacggtgtgc cgtggatcgg tggccaggcc ggcggcgccg ggcagccggt gctccgcttc
     2461 accggcgaca tcgtgcaggc gggctacaac atcaacatga accggccgat cacctcgacc
     2521 gcccccgtgc ctgcggcatc ggccactcgg ctctccgatg tctggccgac gccggccgcc
     2581 gcgaggacct gggtg
//
LOCUS       DBNHQR010000066         2882 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_892551, whole genome shotgun sequence.
ACCESSION   DBNHQR010000066 DBNHQR010000000
VERSION     DBNHQR010000066.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_892551"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..243)
                     /locus_tag="AC16VR_01430"
                     /pseudo
     CDS             complement(1..243)
                     /locus_tag="AC16VR_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012319572.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(498..1292)
                     /gene="istB"
                     /locus_tag="AC16VR_01435"
     CDS             complement(498..1292)
                     /gene="istB"
                     /locus_tag="AC16VR_01435"
                     /inference="COORDINATES: protein motif:HMM:NF013828.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HIR6009841.1"
                     /translation="MSGARPSAARPKLDIDRTREQLLALGCSHAADRLGDALNRAVAK
                     ETPPHAFLDQLLDAELAGREERRIRTALKLSNLPTGQTLENFDFAFQPAIERSRIDTL
                     ATGAWVRGNETVLLQGPPGVGKTHLAVSLGVKTVQLGFSVQYYRFDELMTALRADADM
                     PPARLRRRKYMSTALLIIDELGFEAMSREEASLFFRLITYRYGRGAVLITTNKSVRDW
                     TELLAGDEVLAAAILDRLLHRSHVLNIKGRSYRLRDLEDVLKRAHA"
     gene            complement(1289..2524)
                     /gene="istA"
                     /locus_tag="AC16VR_01440"
     CDS             complement(1289..2524)
                     /gene="istA"
                     /locus_tag="AC16VR_01440"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HIR6009842.1"
                     /translation="MTIKVLGERGTTGSEIARLLGVSEGAVRYHVKRMGSGSVDGRSS
                     QPQKAAAFSAAIEHWRQSAGDAGLNLAALHVWLVREHGYDGSLRSVQRYWKRTYPAPR
                     VRARRRVETPAGAQAQVDWAHFPGVVMGSESVDVVALHMVLSHSRKEAIVWSRSKDML
                     AWITCHLGCFERLGGVPATVRIDNEKTAISKGAGAWGVINPTYRSLARTLKFHVDACP
                     PRQPQAKGKVERRVRDQRHGIDPYGRSFASLGELQAWTDEQVAVRGRHRRCPATGTTA
                     AEAWHAEKRVLTALPDPLPVPFDVVVDRRVGIDALVAFEGRQYSVPFQLVGQTVEVRG
                     IAGAVQLLKNGEIVAQHSRGTDARLLVDQTHYDGPSDDRVIAPPPLGRMGRRLQELID
                     APVACHAIEMYAALAEVAR"
BASE COUNT          511 a          963 c          922 g          486 t
ORIGIN      
        1 cgatggaaag accgcgcgct ttcagatcga cgagcagctc ccgccatgaa aggccagcag
       61 gcctttcatc tggttggcga agccacgcgg gtcagcagac ccgcgaaggc ggggggcgct
      121 ctcgcgctcg ccgacctgga agccgaccag ctccttccta ccctccgggg tggcaccaat
      181 gacgaccagc atgcagctgg cggtgtcctc cattcgggcc tgcagataca cgccgtccgc
      241 ccatcagccg cagcaggcgg ctgatccatt cctgcaagga ccagggccag gaggccctgg
      301 cgggacgtag acatgtgtca agcgacaccc agtcttacga gctaaggcga cacccagatt
      361 tacgagcttc ggtgctgacg agcacgctcg gtgctgaggt cagcggctct tcacttctcc
      421 gagcgaattg acagggtggc tgatgttgct gggcctgtgg agttgtgggc aacgcgtgag
      481 cgttgtccac aaatccatca ggcgtgggcg cgtttgagca cgtcctcgag atcgcgcagg
      541 cggtaggagc ggcccttgat gttgaggacg tgggagcgat gcaggagacg gtcgaggatg
      601 gcggcggcca gcacctcgtc gccggccagg agctcggtcc agtcgcggac gctcttgttg
      661 gtggtgatga ggacggcacc gcggccgtaa cggtaggtga tgaggcggaa gaagagcgag
      721 gcctcctcgc ggctcatcgc ctcgaagccg agctcgtcga tgatcagcag ggccgtggac
      781 atgtacttgc gccgtctcag gcgcgcaggc ggcatgtcgg catcggctct gagcgcggtc
      841 atcagctcgt cgaagcgata gtactggacg gagaagccga gctggacggt cttgacgccg
      901 aggctgacgg cgagatgggt cttgccgaca ccgggcgggc cctgcagcag gacggtctcg
      961 ttgccgcgta cccaggcccc ggtcgccagg gtgtcgatgc gcgagcgctc gatcgccggc
     1021 tggaaggcga agtcaaaatt ctccaatgtc tggccggtcg gtaggttgga gagtttcagc
     1081 gcggtgcgga tgcggcgttc ctcacggcca gccagctcgg catcgagcag ctgatcgagg
     1141 aaggcgtgcg gcggggtttc cttggcgacg gcgcggttca gggcatcgcc gagccgatcg
     1201 gcggcatggc tgcagccgag cgccagaagc tgctcgcggg tccggtcgat gtcgagcttt
     1261 ggcctggctg ccgatggcct tgcaccgctc atcgcgcgac ctccgccaag gcggcgtaca
     1321 tctcgatggc atggcaagcg accggcgcgt cgatcagctc ctgcaggcgc cggcccatcc
     1381 ggcccagcgg cggcggtgcg atcacccggt catccgacgg gccatcgtag tgcgtctgat
     1441 caaccagcag ccgagcatcc gttccgcgcg aatgctgggc gacgatctcg ccgttcttca
     1501 gcagctgcac agcgccggca atgccgcgca cctcgaccgt ttgcccgacc agctggaagg
     1561 gaacgctgta ttggcggccc tcgaaagcca ccagagcgtc gatgccgacc cggcggtcga
     1621 ccacgacatc gaacggaacc ggcagtgggt cgggcagggc tgtgagaacc cgcttctccg
     1681 cgtgccaggc ttccgcagcc gtcgtgcccg tcgctgggca gcggcgatgt cgcccgcgga
     1741 cagcgacctg ctcgtcggtc caggcctgca actcgcccaa agaggcaaag ctccgcccgt
     1801 aaggatcgat gccgtgacgc tggtcacgaa cgcggcgctc gacctttccc ttggcttgtg
     1861 gctgtcttgg cggacaggcg tcgacgtgga acttcaacgt cctggccagg gatctgtagg
     1921 tcgggttgat cacaccccac gcaccggcac ccttcgagat cgccgttttc tcgttgtcga
     1981 tccgcaccgt cgccggcact ccgccgaggc gctcgaagca gccgagatga cacgtgatcc
     2041 aagccagcat gtccttgctg cgcgaccaga cgatcgcctc cttgcggctg tgtgacagca
     2101 ccatgtgtag cgccaccaca tcgacactct cgctccccat gacgacgccg gggaaatgag
     2161 cccagtccac ctgcgcttga gcaccagccg gcgtctcgac ccgacgacga gcccgcacac
     2221 gcggtgccgg ataggtccgc ttccagtacc gctgaaccga gcgcaagcta ccgtcatagc
     2281 catgctcgcg aaccagccag acatgcaaag ctgccaggtt cagcccggca tcacccgcgc
     2341 tctgtcgcca atgctcgatc gcagccgaaa atgccgccgc cttctgtggt tggctgctac
     2401 gaccatcaac agagcccgaa cccatgcgct tgacgtgata gcgcaccgcg ccctcgctca
     2461 cgcccaacaa ccgcgcgatc tcggagcctg tcgtcccacg ttcacccaaa accttgatcg
     2521 tcatgcgagc ctcttcgttg agccggccca tcaaccaccc ctgaaatccc gatgttcagg
     2581 agcttctcag ccggaggcga cacctcgtaa atctgggtgt cgccttcgct cgtcattcgc
     2641 atgtcgccac acacacccag agcgcaggct ccgccgccca aagattgagc gatgacggtg
     2701 acagatgatc gatgccgtgc cgctcaaagg aattcatacg ccgggtctcc ccgcggccgt
     2761 gctgtcacgc atctgctcgg cctcgtagcc ttcgatgtcc tcgaggcgat agacgacccg
     2821 accaccgatc ttgaggaacc gcggcccttc accgagccag cgccagcgct ccagcgtccg
     2881 gg
//
LOCUS       DBNHQR010000067         5306 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_640169, whole genome shotgun sequence.
ACCESSION   DBNHQR010000067 DBNHQR010000000
VERSION     DBNHQR010000067.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5306)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5306)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5306
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_640169"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            443..622
                     /locus_tag="AC16VR_01445"
     CDS             443..622
                     /locus_tag="AC16VR_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009843.1"
                     /translation="MAGGNGLVEMERIAREVEPLFGVGIEIWGFDSGKGLPAPRDYRD
                     LPYIWQAAARVLLHG"
     gene            627..860
                     /locus_tag="AC16VR_01450"
     CDS             627..860
                     /locus_tag="AC16VR_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009844.1"
                     /translation="MSTDTGLLSERVGQRRAIHEYNSVDENRELSPIAGFRHTRAVPA
                     RWNEQVCIHHNYGHPDYCTYIHEQADRQLPLAG"
     gene            complement(866..1324)
                     /locus_tag="AC16VR_01455"
     CDS             complement(866..1324)
                     /locus_tag="AC16VR_01455"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6009845.1"
                     /translation="MSDVKNEDRAAFFEAVDAACKKAVWCALASVAGDRPRVRIVHPT
                     WDGEVLWFATGPDSPKARQLRENPHVDIQYQVAPPDFVHVMVRGTAELVTDQAAKNHA
                     WEAIDYDLTQFGSTGPDDPNFLPVRITPTRVELSEMFGSVNKRVWRAGTP"
     gene            complement(1433..2452)
                     /locus_tag="AC16VR_01460"
     CDS             complement(1433..2452)
                     /locus_tag="AC16VR_01460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6009846.1"
                     /translation="MKDLKLGLQLGYWGAGPNPDMVKVAQEAERLGYDAIFTAEAWGS
                     DVFTPLAWIGAHTERIRLGTGIAQLSARAPTATAMAALTLDHLSKGRVILGLGVSGPQ
                     VVEGWYGRPFGKPLARTREYVSIIRQVLERKEPVTNDGEHYPLPYTGEGSWGLGKPLK
                     SITHPYRSDLPIFLGAEGPKNVAMTAEIADGWLPLYYSPYRQEVYADQIAHAKPGFEI
                     MQGVSVIINDDVEEALIPLKRNLALYIGGMGAKSRNFHNELVQRMGFADAAAKIQDLF
                     LSGRKDDAAAAVPTELADEISLVGSVERIKDRLQAWRETPVTSLLVSTQNISQLRTFA
                     EIVLG"
     gene            complement(2621..3019)
                     /locus_tag="AC16VR_01465"
     CDS             complement(2621..3019)
                     /locus_tag="AC16VR_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009847.1"
                     /translation="MKFVGYLIGSVVILVVLIFVSQLVASESAEVVVLTSQGPDGGQD
                     TRLWVVDRDGLQFLRASPDSGWYQRLAAEPQVLLVRNGNSAAYRAEIRMALAEDVNRL
                     MREKYGWRDAYIEFLIGDRMAAVPVALIPD"
     gene            3167..3904
                     /locus_tag="AC16VR_01470"
     CDS             3167..3904
                     /locus_tag="AC16VR_01470"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6009848.1"
                     /translation="MWIFYSPFHTFIHKVLVTAHECGHWDDITFVATFPFKNNEGIDQ
                     GDAYDISALNPLNKVPTLVTGEGQVIYGSQAACEYLDGTSRARRMYPEPGPARWDAVT
                     RLALSDTIFEQTVVMVMEQWKPESEWNRGLFEWLWPKFIAGLDKLERDAERGWGDFDV
                     GHAAMLHAISYLGFRAEFYEAKDPIHPDFRWRDGRPALSAWFDEAVERPSVQSHYLKD
                     FVGDSSAEFCQAQVQAVLAAQKQHDTG"
     gene            3901..4602
                     /locus_tag="AC16VR_01475"
     CDS             3901..4602
                     /locus_tag="AC16VR_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010485785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6009849.1"
                     /translation="MIDLYYWPTPNGHKVAIMLEEIGSEYQLHPVNILEGEQFDPDFL
                     EISPNNRIPAIVDRSGPDGTPYALFESGAILLYLAEKTGVLWPSSTAARYDMLQWLFF
                     QNANVGPMFGQCGHFQGYAPEPVPYAIERYHNETRRLYGVMDRRLASCAYLGGPAYSL
                     ADIATYPWCMPIIRELHKIDIDEFPNVADWADRIAQRPAVARGTSLLAEVMKIGDPDE
                     KTREAFFGSRQLEQR"
     gene            complement(4614..5012)
                     /locus_tag="AC16VR_01480"
     CDS             complement(4614..5012)
                     /locus_tag="AC16VR_01480"
                     /inference="COORDINATES: protein motif:HMM:NF013228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR6009850.1"
                     /translation="MEPPTEPRLIKTDPDPFEPFCIAQGYQVGNLIFLSGQAALDLQG
                     NVVGSGDFDLQAEQALRNVQMLLDQAGSGLDKLIKVTIYLTDMTQFPKIVELRRKWFT
                     PPYPADTIVEVRSLALPELMIEIDAIALAG"
     gene            5047..>5306
                     /locus_tag="AC16VR_01485"
     CDS             5047..>5306
                     /locus_tag="AC16VR_01485"
                     /inference="COORDINATES: protein motif:HMM:NF019845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR6009851.1"
                     /translation="MRAVYYERFGGSEVLTVGELPTPEPASGEVLIRIHAASVNPVDW
                     KIREGFFDGIFPHRFPIIPGWDADGEVAGLGDGVTEMQIGSRV"
BASE COUNT         1012 a         1731 c         1642 g          921 t
ORIGIN      
        1 cagccaaccg ccgtgatccg gacccctggc aacccaaaag ccatcaggta attaggagga
       61 acgatactgg ataatcggag cttcaatcca gctgcctggg ctcaacccat gggtcgagag
      121 atcaggccct ctagcgacgc cgcgcagtca gcaggatcgg cggcacaaac tgccgcagcc
      181 accaccacgc acttctacaa cgcgctgttc tcctatttct atgtcctgat cgggctcggc
      241 gcttggctca tggcgccaca gcctcacacg gcgcgccgga caagatgggt cgtgctgagc
      301 cctcagcaac cactgagcag cagctgacga gaatggaacg gaacctgccc gcctatctca
      361 aacaaccgag gaaacctcac catgttagac cgcgtggtac cacatctctt gaatccgacc
      421 agagccatcc agcgagcctg gcgtggcggg cggcaacggg ctggtcgaga tggaacgcat
      481 tgccagggaa gtggaaccgc ttttcggcgt cggcatcgag atatggggct tcgacagcgg
      541 caaagggcta ccggcaccga gagactaccg ggatcttccc tacatctggc aggctgccgc
      601 gcgcgttctg ctacatggat gacatcatga gcaccgacac gggcctcctt tcagaacggg
      661 tcgggcaacg gcgcgcgatt cacgaataca attcggtcga cgagaaccgg gagctttccc
      721 ccatcgccgg tttccgccac acccgtgccg tgcctgcccg ctggaacgag caggtctgca
      781 tccatcacaa ctatgggcat ccggactact gcacctacat ccacgaacag gcggaccggc
      841 aactgcccct tgccggctga ggcgtctacg gtgtgcccgc gcgccagacc cgcttgttga
      901 cggacccgaa catctcagag agttctacgc gggtgggcgt aatacgcacc ggcaggaaat
      961 tgggatcatc gggacccgtg gagccgaact gtgtcagatc gtagtcgatg gcctcccagg
     1021 catgattctt ggccgcctga tcggtgacca gctctgccgt tccgcgcacc atcacgtgca
     1081 cgaaatcggg cggcgccacc tggtactgaa tgtccacgtg cgggttctca cgcagctgtc
     1141 tggccttggg tgagtcggga ccggtggcga accagagcac ctccccatcc caggttggat
     1201 ggacgatgcg cactctcggc cgatcgccgg ccaccgatgc aagcgcgcac cacaccgcct
     1261 tcttgcaggc ggcatcgacg gcctcgaaaa aggccgctct gtcttcattc ttaacgtcgc
     1321 tcatcgagct gatcccccat ttgttgtcag cgaaggtgtt gtcagcgagg ggctggaccc
     1381 aacgttggtc tcctgccacg cacctgagat tcggccggcg agcttcaccc gctcaaccga
     1441 gcacgatttc ggcaaacgtt ctcagctgcg agatgttctg ggtggagacc agcagcgagg
     1501 tcaccggcgt ctcccgccat gcctgcagcc ggtccttgat ccgctcgacg gatcccacca
     1561 gggatatctc gtcggcaagc tccgtcggaa ctgccgccgc ggcgtcatcc ttgcgaccgg
     1621 agaggaaaag atcctggatc ttggccgccg cgtcggcgaa gcccatgcgt tgcaccagct
     1681 cgttgtggaa gtttctggac ttcgcgccca tgccgccgat gtacagcgcc aggttacgct
     1741 tcagcgggat caacgcttcc tcgacgtcgt cgttgatgat caccgagacg ccctgcatga
     1801 tctcgaagcc gggctttgca tgggcgatct ggtcggcgta gacctcctgc cgatagggcg
     1861 agtagtagag gggcagccag ccgtcggcga tctccgcggt catggccacg ttcttcggcc
     1921 cctctgcgcc aagaaagatc ggcagatcgg atcggtaggg gtgggtgatg gacttcagcg
     1981 gcttgccaag tccccacgag ccctcacccg tgtacggtaa aggatagtgc tccccgtcgt
     2041 tggtcaccgg ctccttgcgc tcgagcacct ggcggatgat gctcacgtac tcgcgggtac
     2101 gcgcgagggg ctttccgaac gggcgcccgt accagccctc cacgacctgc ggtcccgaca
     2161 ccccgagccc gagaatcacg cgccccttgg aaaggtggtc gagggtcagg gcggccatcg
     2221 ccgtggcggt gggcgcccgg gcggaaagct gagcgatgcc ggtcccgagc ctgatccgct
     2281 ccgtgtgagc ccctatccag gccagcggcg tgaagacgtc cgagccccac gcctcggccg
     2341 tgaaaatcgc atcgtagccc agacgctctg cttcctgagc gaccttcacc atgtcggggt
     2401 tgggtccagc gccccagtaa cccaattgca ggcccagttt cagatccttc acctcaaatg
     2461 ctccttcgct tggctgaact cacggccgcg tgacgacctc acttactgcc gcatctcact
     2521 acgcgggaag caaaagctca agcctcaatc tctccgcggc aaggttcgca ggacatgcag
     2581 agaactcaaa gccggccccg tgcctacgcg gggccggcga tcagtcggga atgagcgcga
     2641 ccggcaccgc tgccatccga tcgccgatga gaaactcgat gtacgcgtcc cgccagccgt
     2701 acttttcccg catcagacga ttgacgtcct cggcgagggc cattctgatc tcggcacggt
     2761 aagcggcgct gtttccgttg cggacgagca gcacctgcgg ttccgccgcc aggcgctggt
     2821 accagccgga gtcagggctg gctcgcagga actgcagccc gtcccgatcg accacccaca
     2881 accgggtgtc ctgcccgccg tcgggcccct ggctggtcaa cacaaccacc tccgccgatt
     2941 cggacgcgac gagctgggag acgaagatca gaacgaccag gatgaccacc gagccgatca
     3001 ggtacccaac gaatttcacc cctgccctcc tcgcggcgcc aggtgtggcc ggaaaggcta
     3061 acggatcgct gccgcgaaat gaactgctgc gccaggctga acgttcgggc atcatcgccc
     3121 cagtgacgtg agggtcggtc cggtagcgaa gagggggaac ctgctgatgt ggatattcta
     3181 ttcacccttc cacactttca ttcacaaggt gctggtcacc gctcacgagt gcggccactg
     3241 ggacgacatc accttcgtcg ccacgtttcc cttcaagaac aacgagggca tcgaccaggg
     3301 cgacgcctac gacatctcag cactcaaccc tctgaacaaa gtgccgaccc tggtcaccgg
     3361 ggaaggccag gtcatctacg gcagccaggc ggcctgcgaa tatctcgacg gcaccagcag
     3421 ggcccgccgc atgtaccccg agcccggccc ggcgcgatgg gacgcggtga cgcgcctggc
     3481 gctcagcgac accatcttcg agcagacggt ggtcatggtg atggagcagt ggaagccgga
     3541 aagcgagtgg aaccgcggcc ttttcgagtg gctgtggccc aagttcatcg ccgggctgga
     3601 caagctggag cgggacgccg agcggggctg gggcgatttc gacgtcggcc acgcggctat
     3661 gcttcatgcg atcagctatc tgggattcag ggccgaattc tacgaggcca aggatccgat
     3721 ccatcccgat ttcaggtggc gcgacggccg accggcgctg tccgcctggt tcgacgaagc
     3781 cgttgagcgt ccctcggtcc agtcccacta cctgaaggat ttcgtcggcg actcgagcgc
     3841 cgagttctgc caggcacagg tgcaggccgt gctggcagcg cagaaacagc acgacacggg
     3901 atgatcgacc tctactactg gccgacgccc aacggccaca aggtcgccat catgctcgag
     3961 gagatcggca gcgagtacca gctgcacccg gtcaacatcc tcgaggggga gcagttcgat
     4021 cccgacttcc tcgagatcag ccccaacaac cgcatccccg ccatcgtgga ccgcagcgga
     4081 cccgacggga cgccctacgc gctgttcgaa tccggcgcga ttctgctcta cctggcggag
     4141 aagacgggcg tcctgtggcc gtcgagcacg gcggcgaggt acgacatgct gcagtggttg
     4201 tttttccaga atgccaacgt cggccccatg ttcgggcagt gcggccactt ccagggctac
     4261 gcgcccgaac cggtgcccta cgccatcgag cgctatcaca acgaaacccg acggctctac
     4321 ggcgtgatgg accggcgcct ggcgagctgc gcctacctcg gggggccggc ctactcgctg
     4381 gcagacatcg ccacctatcc ctggtgcatg ccgatcatcc gggagctgca caagatcgac
     4441 atcgatgagt ttcccaacgt ggccgactgg gcagaccgca ttgcgcagcg cccggccgtg
     4501 gcgcggggca cgtctctgct cgcagaagtc atgaagatcg gcgacccgga cgagaaaacg
     4561 cgcgaagctt tcttcggatc ccgacagctg gagcaacgct gaggggaagc gcctcagccg
     4621 gccagggcga tggcgtcgat ttcgatcatc agctccggca gggccagcga acggacctcg
     4681 acgatggtgt ccgccgggta cggcggcgtg aaccactttc gccgcagctc cacgatcttc
     4741 gggaactgcg tcatgtcggt cagatagatg gtcaccttga tcagcttgtc gaggccggag
     4801 cctgcctgat cgaggagcat ctgaacgttg cgcagcgcct gctcggcctg caggtcgaag
     4861 tcgccggaac cgacgacgtt cccctggaga tccagcgccg cctggccgga caggaaaatc
     4921 aggttgccca cctgataacc ttgggcgatg cagaacggct cgaacgggtc aggatccgtc
     4981 ttgatcaggc ggggttcggt gggcggctcc atgagggctc cattggttcg aagaggggca
     5041 gattgaatgc gtgcagtcta ctacgagcgg tttggcgggt ccgaggttct cacggtcggc
     5101 gagttgccga cacccgagcc cgcctcggga gaggtgctca tccggattca cgcggcaagc
     5161 gtcaacccgg tggactggaa gattcgcgag ggcttcttcg acggaatctt tccccaccgg
     5221 tttcccatca tcccgggctg ggatgccgac ggagaggtcg ccggtctggg ggacggggta
     5281 actgaaatgc agatcggcag ccgggt
//
LOCUS       DBNHQR010000068         2719 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_6758, whole genome shotgun sequence.
ACCESSION   DBNHQR010000068 DBNHQR010000000
VERSION     DBNHQR010000068.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_6758"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="AC16VR_01490"
     CDS             <1..386
                     /locus_tag="AC16VR_01490"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002016.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HIR6009852.1"
                     /translation="RGSDMAHGGDAKRLAEMRCEPCRGDSPAVAPDAREAMLAELTGW
                     RVDVVDEVPRLSRTYQTADFAAALGLANTIGEMADSEDHHPQLVVEWGRLQVSWWTHA
                     IGGLHMNDFVLAARCDELAAKAASD"
     gene            complement(383..1468)
                     /locus_tag="AC16VR_01495"
     CDS             complement(383..1468)
                     /locus_tag="AC16VR_01495"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008171726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR6009853.1"
                     /translation="MKYLTDDVRIIGMEEVTAPETLLSELPITDSVSRLVFSNRKQIS
                     EILHGSDPRLLVVVGPCSIHDPEAALDYAKRLAPVAKDLSDRLHVIMRVYFEKPRTTV
                     GWKGLINDPHLDNSFDINNGLRIARGVLRDIAECGLGAGTEYLDPITPQYVGDLVAWG
                     AIGARTTESQIHRQLASGLSCPVGFKNSTDGSIQVAVDAIKSAANSHIFLSVTKSGHS
                     AIFSTAGNDDCHLILRGGGGKPNYDNPSVHYASRLLEQAGLTSRLMIDMSHANSGKDH
                     NQQIRVCQDICKQVREGESRIFGVMIESNLVPGNQKIGPRESMTYGQSVTDACIGWED
                     TVTCLTALADAGGARMEGSAAAAAGSG"
     gene            1578..2234
                     /locus_tag="AC16VR_01500"
     CDS             1578..2234
                     /locus_tag="AC16VR_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009854.1"
                     /translation="MTEAVRLDARRRGMLADMGIDVWVIRGNAAGVVEVPQSQAIVVP
                     EPVPPAETKAPEKGPDVARLNDSAPSAAQQAKMPVPRRRPLEHPEADVATEIAVTCLT
                     RPGALLLAYGREWTQAGRLGRDLLAAATGSWGSDPKRISFGWREPGDPSDGWRAFKAF
                     ADKQLADAEASVIFVNEALVDHLPERASSGTLVVLPNLAEFDTDAKRGLWTRIRNLGR
                     "
     gene            2249..2698
                     /gene="rimI"
                     /locus_tag="AC16VR_01505"
     CDS             2249..2698
                     /gene="rimI"
                     /locus_tag="AC16VR_01505"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 - protein-N-terminal-alanine
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HIR6009855.1"
                     /translation="METADLDAVLEAEFLSYAFPWTRGVFLDCLKARHECWVGCLGDA
                     PVAHAVVSVGAGESHLLNLCVRRDLQGRGYGRALAVHVVGRARARGASAMFLEVRPSN
                     LVAAELYESLGFREIGIRKNYYPAQVGHEDARVLALDLDAHFRSDPL"
BASE COUNT          448 a          807 c          953 g          511 t
ORIGIN      
        1 agagaggatc agacatggcg catggtgggg acgcaaagcg gctggctgag atgcggtgcg
       61 agccttgtcg gggcgattcg ccggcagtgg cgccggatgc gcgggaggct atgctggccg
      121 agctcaccgg ctggcgggtc gacgtggtgg acgaggtacc caggctcagc cggacctatc
      181 aaaccgccga cttcgccgcg gcgctggggc ttgcgaacac catcggcgag atggccgaca
      241 gtgaggatca ccatccgcag ctggtggtgg agtggggccg gctgcaggtc agctggtgga
      301 cgcacgccat cggcggcctg cacatgaacg acttcgttct cgcggcccga tgcgacgagc
      361 tggctgccaa ggcggcttcg gactagcccg atcctgccgc tgcagcggcc gagccttcca
      421 tccgcgcccc gcccgcgtcg gccagcgcgg tcaggcaggt gactgtgtcc tcccacccga
      481 tacacgcatc agtgacgctc tggccgtacg tcatggattc acgtgggccg atcttctggt
      541 tgcccgggac caggttgctc tcgatcatca cgccgaaaat ccgtgactcg ccttcgcgga
      601 cctgcttgca gatatcctgg cagacccgga tctgctggtt gtgatccttg ccgctgttgg
      661 cgtggctcat gtcgatcatc aggcggctcg ttagccccgc ctgctcgagc agacggctgg
      721 catagtggac cgaagggttg tcgtagttcg gcttgccgcc cccgcctcga agaatgagat
      781 ggcaatcgtc gttgccggcc gtggaaaaga tggccgagtg cccggacttg gtgacggaga
      841 gaaatatgtg ggaattggcg gccgacttga tggcgtccac ggcgacctgg atggagccgt
      901 cggtgctgtt cttgaaaccc acggggcagg acagcccgga agcgagctgg cggtgaatct
      961 ggctctcggt ggtgcgggct ccgatagcgc cccaggctac caggtcgccg acgtactgcg
     1021 gcgtgatcgg atccaggtac tccgtgcccg cgcccagtcc gcattcggct atgtctctga
     1081 gcacgcccct ggcgatcctc agaccattgt tgatgtcgaa ggagttgtcg agatgggggt
     1141 cgttgatcag gcccttccac cccacggtcg tgcgcggttt ttcgaagtag acccgcatga
     1201 tgacgtgaag acggtcggag aggtctttcg ccaccggggc cagccgcttt gcatagtcca
     1261 gggcagcttc cgggtcatga atcgagcagg gcccgacgac caccagcaat cgggggtcgc
     1321 tgccgtggag aatctcgctg atctgcttgc ggttggagaa gaccagtcgc gaaacggaat
     1381 cggtgatcgg cagctcggaa aggagggttt ccggcgcggt gacctcttcc atgccgatga
     1441 tgcgtacgtc gtctgtcaga tacttcattg ggaaccatca cggtgcgtga gctcggaagc
     1501 tagagtgtac aatgaccgca gaggcagggt taccggcaat cgcggcatgt ataccaaagt
     1561 tctgaacggg tgcgggcatg actgaagccg ttcgtctgga tgcgcggcgt cgggggatgc
     1621 tcgcggacat gggcatagac gtctgggtta tcaggggtaa cgcggccggg gtcgtcgagg
     1681 tcccgcagtc gcaggccatc gtcgtgcctg agcccgtgcc gccggccgag acgaaggcgc
     1741 cggagaaggg ccccgacgtt gccaggctga acgactcggc gccttcggcg gcccagcagg
     1801 cgaagatgcc ggtcccgcgc cggagaccct tggaacatcc ggaagcggat gtcgcgacgg
     1861 aaatcgcggt cacctgtctg acgcgccccg gcgctttgct gctggcttac ggccgggaat
     1921 ggacccaagc cggcaggctc ggccgcgacc tcctcgctgc cgcgaccggc agctggggct
     1981 cggatccaaa gcgaatctca ttcggctggc gggagccggg cgatccttcg gacggctggc
     2041 gggccttcaa ggcatttgcg gacaagcagc tggcggacgc cgaggcttcg gtgattttcg
     2101 taaacgaagc gctggtcgat caccttccgg agcgggcgag ctccgggacg ctggtcgtgc
     2161 tgccgaactt agccgagttc gacactgacg ccaaacgagg cctatggacc aggatccgga
     2221 atctcgggcg ctgatcaacc ttcggcccat ggagacggcg gatctcgatg ccgttctgga
     2281 agccgagttt ttaagctacg cgtttccgtg gacgcgcggg gtctttctgg attgtctgaa
     2341 ggctcggcac gaatgctggg tcggctgcct cggcgacgct ccggtggcgc acgccgtggt
     2401 gtccgtgggg gccggcgagt cgcatctgct caatctgtgt gttcgcaggg accttcaggg
     2461 acggggttac ggccgtgctc tagcggtgca cgtcgtgggg cgggcccggg cgcggggcgc
     2521 gtcggccatg ttcctcgagg tccggccctc taatctggtg gccgccgaac tgtacgagtc
     2581 gctcgggttc cgggaaatcg gaattcgcaa gaattactat ccggcccagg tgggtcatga
     2641 ggacgcccgg gtactcgcgt tggatcttga tgctcatttc cgcagcgacc cgctataatc
     2701 cgcgccgttt tccggcggg
//
LOCUS       DBNHQR010000069         3694 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_767751, whole genome shotgun sequence.
ACCESSION   DBNHQR010000069 DBNHQR010000000
VERSION     DBNHQR010000069.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_767751"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="AC16VR_01510"
     CDS             complement(<1..267)
                     /locus_tag="AC16VR_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009856.1"
                     /translation="MARVKLAKPESVDDPAVAGIFEWVTQMEGSVPNHFYVEMNFPEF
                     FTAKLGATKVLWEAGELTMPEIQHIGILVSKDNGCRYCTAAFCTI"
     gene            complement(301..2715)
                     /locus_tag="AC16VR_01515"
     CDS             complement(301..2715)
                     /locus_tag="AC16VR_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6009857.1"
                     /translation="MNSTRSGLGRRLSAPAAFLGLACAFSGVAQSAHSAASSVIEEIV
                     VTARMREEGLQSAPIAISAFTEETLDYRGVERLDEIAKFVPSLTLENNPSFGGASNSA
                     AIYIRGIGQKEFLPTTEPGVGLYVDGVYVARSVGAILDIIDVERLEVLRGPQGTLFGR
                     NTIGGAISITTVKPEPGGEFEAMVSGALGTDDLVHLRGSAHLPLSDTVAARLSVASFQ
                     QDGYVDRADGIDLGEDDTLTGRLAIAWEPNDRFRADLSLEATRDRENGPPMELIGIDF
                     TDLSQLQGVVLAPPPPMAFIHNVTAAAAGPGQPCAVTDVNGNGITYNPSIPNCYDSRY
                     IDPESGENQGTAPAFSKTDLFGVSANLRWDLSDTLTLKSITSWRDLDSEFARDGDHSP
                     QRISQFFDDLEQQQFTQEFQLLGAHERLNWILGAYYFTEDGDNVNILDFTVSNFRSGG
                     EFDNEAWAVFAQATFDITDRLHLTAGGRYTEEEKSFLPDQIIFQNYYAGISELVPEGN
                     PLAALDAPFLQAGERILPYLEKRIDIDEFTPMVNVSFDATDDVMLYATYSEGFKSGGF
                     TQRVFPPIVPGFTAPPGTPDIDLIPTYEPEFVKSYEIGAKATGWDGRLRLNGAVFRSD
                     YKDLQVQVFNSVAPVTQNIGKASINGLELEVTLVPADDWLIEGSLSWLDAEYDEIDTG
                     ITLIGEDNEFERVPEFTASAGISKEFQLDDAGSLVVRADWSYRSETFNDAYNTPQLET
                     DGYHLIDASVRWRNAGEDWTVALSGRNLTDEKYLVSGVYGTAFQSYEGMFDRGSQWRL
                     EVRKEF"
     gene            complement(2801..>3694)
                     /locus_tag="AC16VR_01520"
     CDS             complement(2801..>3694)
                     /locus_tag="AC16VR_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008175355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - L-tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HIR6009858.1"
                     /translation="EVWQVAASYHAKPYRRTMMEIWGGKVHPSPSEVTEFGRSLLAEN
                     PDHPGSLGIAISEAVSQAVADPQTRYALGSVLNHVLLHQTIIGEEALLQLAKVGETPD
                     VLVGCTGGGSNFGGLSFPFLREKLAGRINPTIRCVEPTACPTLTKGQYRYDFGDTAGL
                     TPLMKMHTLGHSFIPEPIHAGGLRYHGMAPLVSHLYELGMVEAIAIPQTECFEGALQF
                     ARTEGIVPAPEPTHAIAAAIREALACRESGEEKVILTALCGHGHLDLASYDKYLHGEM
                     VDLDLSDEVIEAAMKDVPVLA"
BASE COUNT          635 a         1130 c         1213 g          716 t
ORIGIN      
        1 gatggtgcag aatgccgccg tgcagtagcg gcagccgttg tcttttgaga ccaggatgcc
       61 gatgtgctgg atctcgggca tggtcagctc accggcctcc cagagcacct ttgtggcgcc
      121 gagcttcgcc gtgaagaact ccgggaaatt catctccacg tagaaatggt tgggcacgct
      181 gccctccatc tgggtcaccc actcgaagat gccggctact gccggatcgt caacggactc
      241 aggcttggcg agtttcactc ttgccatgtt tcggcctctc gtcgtcccct gtgaatcggg
      301 ctagaactcc ttgcggactt ccagccgcca ctggctgcca cgatcgaaca tcccttcgta
      361 ggactggaag gccgtgccgt agacgccgct gacgagatac ttctcgtcgg tcaggttgcg
      421 gcctgacagg gcaacggtcc aatcctcgcc cgcgttgcgc cagcggacgc tggcgtcgat
      481 caggtggtag ccgtccgtct ccagctgcgg cgtgttgtag gcatcgttga aggtttccga
      541 gcggtagctc cagtcggcgc gcactaccag cgagcccgca tcgtcgagct ggaactcctt
      601 ggagatgccg gcgctggccg tgaactccgg cacccgctcg aactcgttgt cctcgccgat
      661 cagcgtgatg ccggtgtcga tctcgtcgta ctcggcgtcc agccacgaca ggctgccttc
      721 tatcagccag tcgtccgccg gcaccagggt cacctccagc tccaggccat tgatggacgc
      781 cttgccgatg ttctgggtga cgggcgcgac gctgttgaac acctgtacct gcagatcctt
      841 gtagtcgctt cggaacacgg cgccgttcag acgcagccgg ccgtcccaac ccgtggcttt
      901 ggcgccgatc tcgtaggact tcacgaattc aggttcgtac gtggggatca ggtcgatgtc
      961 gggcgtcccc ggcggcgcgg taaatccggg aacgatgggc gggaagacgc gctgggtgaa
     1021 gccccccgac ttgaaccctt cggagtaagt ggcgtagagc atgacgtcgt cggtcgcatc
     1081 gaacgagacg ttcaccatgg gggtgaactc gtcgatgtcg atgcgtttct cgaggtacgg
     1141 caggatccgc tcgcccgcct ggaggaaggg cgcatccagc gcggccaggg ggttgccctc
     1201 gggtaccagc tcgctgatgc ccgcatagta gttctggaag atgatctgat ccggcaggaa
     1261 ggacttctct tcctcggtgt agcggccgcc cgcggtcagg tgcagccggt cggtgatgtc
     1321 gaaggtcgcc tgggcgaaga cggcccacgc ttcgttgtcg aactcgccgc cggagcggaa
     1381 gttggagacg gtgaaatcca ggatgttcac gttgtcgccg tcctcggtga agtagtaggc
     1441 gcccaggatc cagttcagcc gttcgtgagc accaaggagc tggaattcct gggtgaactg
     1501 ctgctgctcg agatcgtcga agaactgcga gatgcgctgg ggcgagtgat ccccgtcccg
     1561 ggcgaactcg gagtcgagat cccgccagct cgtgatggac ttcagcgtca gcgtatcgga
     1621 gaggtcccag cgaaggtttg cggacacgcc gaagaggtcc gtcttggaga acgcgggcgc
     1681 ggtgccctga ttttcgccgc tttccggatc gatgtagcgg ctgtcgtagc agttcgggat
     1741 gctcgggttg taggtgatgc cgttgccgtt gacgtcggtg acggcgcagg gctggcccgg
     1801 cccggccgcg gcggcggtga cgttgtgaat gaatgccatg ggcggaggcg gtgccagaac
     1861 cacaccctgc agctggctga ggtcggtgaa gtcgatgccg atcagctcca tgggcggacc
     1921 gttttcgcgg tcccgtgtgg cttccaggct gaggtcggcg cgaaagcggt cgttgggctc
     1981 ccacgcgatt gccaaacgcc cggtcaacgt gtcgtcctcg cccagatcga tgccgtcggc
     2041 acggtccacg tacccgtcct gctggaagga tgcgacggac agccgggcgg ccaccgtatc
     2101 gctcagcggc aggtgggccg agccgcgcag atgcaccaga tcgtcggtgc ccagagcgcc
     2161 ggataccatg gcttcaaact cgccgcctgg ctcgggtttc accgtggtga tggatatggc
     2221 gccgccgatt gtgttccggc caaacagggt gccctggggg ccgcgcagga cttccaggcg
     2281 ttccacgtcg atgatgtcca ggatcgcgcc aaccgagcga gccacgtaca cgccgtccac
     2341 gtacagaccc acgccaggtt ccgtggtggg cagaaactct ttctgaccga tgccgcggat
     2401 ataaatggcg gcagagtttg aggcaccacc gaaactcggg ttgttctcca gcgtcaggct
     2461 gggcacgaat ttcgcgatct cgtccagacg ttccaccccc cggtaatcga gcgtctcctc
     2521 ggtgaatgcc gaaatggcga tgggcgcgct ctgcagcccc tcttcacgca tgcgggcagt
     2581 gaccacgatt tcctcgatca cgctggacgc agcgctgtgc gccgactgag cgactcccga
     2641 aaaagcacag gccaggccga gaaacgcggc cggcgcggac agacggcggc ccaggccgct
     2701 ccgagtagag ttcatgatgg ttctcccctg attgcttgtt ctgtcgtcgt tcgttcggtg
     2761 gtgctcgttt aaaagcgggt gttcgctcgt ctaggcccgc tcatgcgagg acgggcacgt
     2821 ccttcatggc cgcctcgatg acttcgtcgg agagatcgag atcgaccatt tctccgtgca
     2881 aatacttgtc ataggacgcc agatccaggt ggccgtggcc acacaaggcg gtcaggatga
     2941 ccttttcctc accggattcc cggcaggcga gtgcctcgcg aatggcggcc gcgatggcat
     3001 gagtcggctc gggggccggc acgatgcctt cggtacgggc gaactgcagc gcgccctcga
     3061 aacactccgt ctgtggaatt gcgatcgcct ccaccatgcc gagctcgtag agatgcgaga
     3121 ccagcggcgc catgccatgg tagcgcaggc cgcctgcgtg gatcggctcc ggaatgaaag
     3181 agtggccgag cgtgtgcatc ttcatcagcg gcgtcaggcc cgcggtgtcg ccaaagtcgt
     3241 agcggtactg acctttggtc agagtcgggc aggcagtggg ttccacgcag cgaatggtag
     3301 ggttgatgcg gccggcgagt ttctcgcgca ggaacggaaa agacagcccg ccgaaattcg
     3361 atccgccgcc ggtgcaaccc accagcacgt ccggggtttc gccgactttc gcaagctgca
     3421 gcagtgcttc ctctccgatg atcgtctggt ggagcagcac gtgattgagc acgctgccga
     3481 gcgcgtaacg tgtctgcgga tcggcgaccg cttggctcac cgcttcagag attgcgatcc
     3541 ccagcgatcc gggatggtcg gggttctcgg caagcaggga acggccgaac tccgtgactt
     3601 cagaggggct cgggtgcacc ttaccgcccc agatttccat catcgtccgc cggtaaggct
     3661 tggcatgata ggaagccgcc acctgccaga cctc
//
LOCUS       DBNHQR010000070         6797 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1337325, whole genome shotgun sequence.
ACCESSION   DBNHQR010000070 DBNHQR010000000
VERSION     DBNHQR010000070.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6797)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6797)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6797
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1337325"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1668
                     /gene="priA"
                     /locus_tag="AC16VR_01525"
     CDS             <1..1668
                     /gene="priA"
                     /locus_tag="AC16VR_01525"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication restart helicase PriA"
                     /protein_id="HIR6009859.1"
                     /translation="RAAAGKGLITRTEAATGFTLGIDEQPPELSDEQAGVLSDLLART
                     DCFAPSLLEGITGSGKTEIYLQLIDNVRKAGHQVLVLVPEIALTPQTVGRFERRFGSG
                     GGVASLHSNLGDQERLQTWLKCRSGEVSVLIGTRSAVFAPFAKLGLIVVDEEHDGSFK
                     QQDGLRYSARDVAVKRAQTLDIPLLLGSATPSLESLNNAMAGRYAHLRLRHRAGGARP
                     PRFHLLDIRGQSLDDGLSQDLLRIVGQHLNRGGQALVFLNRRGYAPTYLCPRCSWQAA
                     CGHCEMRLTLHLSPRALLCHHCGHQRPLPSACPDCGHAELIAVGAGTQRTEAGLAKRF
                     PGIPLYRVDRDTTRSQRRMEAQFEAIHKGDPAILVGTQMLAKGHHFPKVTVVAVVNAD
                     GGFLSADYRAPERTAQLITQVAGRAGRAEDPGEVWIQTYQPDGVLLRSLIDEGYDGFA
                     RRELAIRKSSGLPPFRPMALLRAESRDAESARNFLTSVRDRLGSPLEVLGPAPAPIAR
                     VANRLRFQLMVMADSRRELHAGIRPIRDLTAPGTIRWSVDIDPYDTF"
     gene            1887..2312
                     /locus_tag="AC16VR_01530"
     CDS             1887..2312
                     /locus_tag="AC16VR_01530"
                     /inference="COORDINATES: protein motif:HMM:NF016895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR6009860.1"
                     /translation="MAVALNLPDPQPERPPAAATAASAPPGSVTFEFDKDLRDAFVEA
                     DPGQYETDAGHDGSRPVSYLIQAASFPVAADADSLQAELAQMGLAAQSVPARLNAGTW
                     FRVTVGPFDSQLKANRALTQLREIDLDAFMIQQRLPEPR"
     gene            2423..2965
                     /gene="hslV"
                     /locus_tag="AC16VR_01535"
     CDS             2423..2965
                     /gene="hslV"
                     /locus_tag="AC16VR_01535"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014754676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HIR6009861.1"
                     /translation="MADIHGTTIVSVRNDRQVAIGGDGQVSMGQTIMKGNARKVRRLH
                     DDKVLAGFAGGTADAFTLFERFEGVLDKYHGNLTRAAVELAKDWRSDRALRRLEALLV
                     VADREATLLVSGTGDVIEPEAGVIAIGSGGPFAQAAARALNENTELDPADIVEKSLSI
                     AADICVYTNHHRTIEVLDLT"
     gene            2971..4314
                     /gene="hslU"
                     /locus_tag="AC16VR_01540"
     CDS             2971..4314
                     /gene="hslU"
                     /locus_tag="AC16VR_01540"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019650789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HIR6009862.1"
                     /translation="MTPREIVHELDKHIIGQDDAKRAVAIALRNRWRRMQLEEEMREE
                     ISPKNILMIGPTGVGKTEIARRLARLANAPFIKVEATKFTEVGYVGRDVESIIRDLAD
                     VAVKMLREEQMDRVMHQAEDRAEERILDALLPGPKPTGSQTIGFTQESSEDEGDSTTR
                     QLFRKRLRQGELDEKEIEIETDAAQVGVEIMAPPGMEEMTSQLQSMFSNLGGGRKRSV
                     RLKIKDALRKLKEEEAAKLVNEDEIKARAVETVEQMGIVFLDELDKVAKRSESAGADV
                     SREGVQRDLLPLIEGCTVSTKYGMVKTDHILFIASGAFHLAKPSDLIPELQGRLPIRV
                     ELAALSAGDFKRILVEPNASLTEQYQALLGIDGLSLEFTDDGIERIAELAWQVNESTE
                     NIGARRLHTVLERLLENVSFTATESAESTVVIDAVYVDAQLSELAQDRDLSRYIL"
     gene            4331..4744
                     /locus_tag="AC16VR_01545"
     CDS             4331..4744
                     /locus_tag="AC16VR_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019412278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-butyrobetaine hydroxylase family protein"
                     /protein_id="HIR6009863.1"
                     /translation="MQRPKQITYRRQSKVLVLSFEDGDAEIPAELLRVYSPSAEVRGH
                     SAAERKLQTGKKHVGIKSLEPVGNYAIRITFDDGHDTGLYGWDLLRDLAENADGYWKS
                     YLDELKAANASRLPTIPVGHWTPASQPAVPPEQDQ"
     gene            4741..5484
                     /locus_tag="AC16VR_01550"
     CDS             4741..5484
                     /locus_tag="AC16VR_01550"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008096122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6009864.1"
                     /translation="MSDQNSVDFGSRKVSPDEKTRLVGGVFASVAERYDVMNDLISLG
                     THRLFKRMTVELSGVREGMRVLDLAGGTGDLSALFSGAVGPSGVVVLADINARMMDVG
                     RNRLLDQGCANVSFCQAAAEALPFADDSFDCVSIGFGLRNFTDKRVALEELNRVIAPG
                     GVLLILEFSRPENAFLEGAYGTFQSLWPGLGKLVAGDARSYQYLIESIRNHPPQKALK
                     LMLEDAGFAAVEYHNLLGGIAAIHRGVKP"
     gene            5481..6119
                     /locus_tag="AC16VR_01555"
     CDS             5481..6119
                     /locus_tag="AC16VR_01555"
                     /inference="COORDINATES: protein motif:HMM:NF014132.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HIR6009865.1"
                     /translation="MSTADVFESALVELVNRAANATLELDPKAASRLAAAEGHRARLI
                     AALPGGREQSFTLSVVGGRMAFTPHAEAEPNVVVRGSVPALLSMLAGGRSAGPVTIEG
                     DVSVLEELAAIFKGFAPDLAEPLSKLVGETSADGILNAAEAGLALLKSAAEATGESLR
                     RGVGERFATRTRVNVLLDRLEDARERSDRLAARIGNLESARGSHQRPEPPTP"
     gene            6116..>6797
                     /locus_tag="AC16VR_01560"
     CDS             6116..>6797
                     /locus_tag="AC16VR_01560"
                     /inference="COORDINATES: protein motif:HMM:NF015094.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HIR6009866.1"
                     /translation="MSALRRLFRVLWIVSRYRLDTLIDADQEGAALPRRYAWLLRASP
                     ARLLPVGDEPRGRRLLLALEQLGPVFVKFGQLLSTRRDLIPLDIADELSALQDRVPPF
                     PGEQAVRIIEDALGQPISEAFRSFEIEPMASASVAQVHAAILPDGKSVVVKVVRPDIA
                     DVIRADLELLRAAAEILENASEEARRLHLKQVVKDYEQTILGELDMQKEADNTARLRS
                     NFAESELLY"
BASE COUNT         1224 a         2261 c         2229 g         1083 t
ORIGIN      
        1 cgcgctgccg caggcaaggg gctgatcacc cgaaccgagg ccgccacggg ctttaccctg
       61 ggcatcgacg agcagccgcc ggagctgagc gacgagcagg ccggcgtgct ctccgacctg
      121 ctcgctcgca ccgattgctt tgcgccatca cttctggagg ggataacggg cagcggcaag
      181 accgaaatct acctgcaact gatcgataac gtgcggaaag ctggccacca ggttctcgtg
      241 ctcgtccccg agatcgcgct cacccctcag accgtcgggc gcttcgaacg ccgcttcgga
      301 tccggcggcg gggtggccag cctccattcc aacctgggtg atcaggagag gttacagacc
      361 tggttgaagt gccgcagcgg cgaggtcagc gtgctcatcg gcacgcgctc cgccgtgttc
      421 gcgccgttcg cgaagctggg cctgatcgtg gtggacgaag agcacgacgg ctccttcaag
      481 cagcaggacg ggctgaggta ctcggctcgt gacgtggcgg tgaaacgtgc gcagacattg
      541 gacataccgc tgctgctcgg gagcgccacg ccttcactgg aaagcctcaa caacgccatg
      601 gccggccgct acgcccacct tcggctgcgc caccgggcgg ggggcgcccg gccgccccgg
      661 ttccacctgc tcgacatccg cggtcagtcc ctcgatgacg gcctcagcca ggatctgctg
      721 cgcatcgtgg gccagcatct caaccggggc gggcaggccc tggtctttct gaaccgacgc
      781 gggtacgcgc ccacctacct gtgtccccgc tgcagctggc aggctgcctg cgggcactgc
      841 gagatgcgac tgacgctgca cctgtcgccg cgggcgctgc tctgccacca ctgcggccat
      901 cagcgaccgc tgccgagcgc ctgcccggac tgcgggcacg cggagctgat tgccgtcggt
      961 gccggtactc agcgcaccga ggccgggctg gctaagcggt tcccagggat tcctctgtat
     1021 cgtgtcgacc gcgataccac ccgaagccag cgtcgcatgg aggcccagtt cgaggccatc
     1081 cacaagggcg atccggcaat cctcgtcggc acgcagatgc tggccaaagg gcaccacttt
     1141 ccgaaggtta ccgtggtggc cgtggtcaac gccgacggcg ggttcctcag cgccgactac
     1201 cgcgcacccg agcgcacggc ccagctcatc acccaggtcg ccgggcgcgc gggtcgtgcc
     1261 gaggatcccg gcgaggtgtg gatccagacc tatcagccgg acggcgtgct gctgcgctcg
     1321 ctcatcgatg agggctacga cgggttcgcc cggcgggagc tggcgattcg caagtccagc
     1381 ggcctgccgc ccttccggcc catggcgttg ttgcgggcgg agagccggga cgccgagagc
     1441 gcccgaaatt ttttgaccag cgtccgcgac cgcctgggaa gccccttaga agtgctcggc
     1501 ccggctccag cgcccatcgc ccgagtagcc aatcgcctcc ggtttcagct catggtgatg
     1561 gcagactcgc gccgcgagct gcatgctggc atccggccga tacgagacct cactgcgcca
     1621 ggcaccatcc gctggtctgt ggacatcgat ccctacgata cgttctgacc tttaccggcc
     1681 gccccaacgg gcgttcaccg gacatcgtgg cggacgaccc gccgcgagcc tatcattcgc
     1741 agcccatggc caacgatttt gcaaaccgac tcaatccgcc gacgacccgg cgccccaagg
     1801 cccggccgcc ctccgcgaca cgtcgcaagg agaggcgttt cagcggttcg agcttcgctt
     1861 ccggtctgct gatcggcggc gcgtgcatgg ccgtggcttt gaacctccct gacccgcagc
     1921 ccgaacgccc gcccgccgca gctaccgcgg caagcgcgcc gcccggatcc gtgaccttcg
     1981 agttcgataa ggatttgcgc gacgcctttg tggaggcgga ccctgggcag tacgagacgg
     2041 acgccggcca cgacggatcc cgaccggtca gctatctcat ccaggcggcg tcgtttccgg
     2101 tagccgccga tgccgactcc cttcaggcag agcttgcgca aatgggcctg gcggcccagt
     2161 cggttccggc gcggcttaac gccggcacct ggttccgggt caccgtcggc ccgttcgaca
     2221 gccagctgaa ggccaatcga gcgcttacgc agcttcgcga aatcgatctg gacgcgttca
     2281 tgatccagca gcgcctgccc gaaccccggt gaccaaaccc cgttggcgga agtccgttgg
     2341 cggaaccccg atggcggaac cccgttgaaa tgccgggcag agggccccac tatgaccagt
     2401 tcgcaatcgg ttgaagagct aaatggcgga tatccacggg acgaccatcg tcagcgtgcg
     2461 caacgacagg caggtggcca ttggcggaga cggtcaggtc tccatgggcc agaccatcat
     2521 gaagggcaat gcccgcaagg tgcggcgcct gcatgacgac aaggtcctgg ctggtttcgc
     2581 cggcggaacc gccgacgcct tcaccctttt cgagcgcttc gagggcgtcc tcgacaagta
     2641 tcacggcaac ctcacccgtg ccgccgtgga gctggccaag gactggcggt cggaccgcgc
     2701 ccttcgacgt ctcgaggccc ttctggtggt ggcggaccgg gaggccacgc tcctggtgag
     2761 cgggaccggc gacgtcatcg agccggaagc cggcgtgatc gccatcggct ccggcggccc
     2821 ctttgcccag gcggcagcgc gggccctgaa cgagaacacg gaactggatc ccgccgacat
     2881 cgtcgagaaa tccctgtcca tcgccgcgga catctgcgtc tacaccaacc atcaccggac
     2941 catcgaggtg ctggatctga catgacccag atgacgccga gagagatcgt tcacgagctc
     3001 gataagcaca tcatcggcca ggacgatgcc aaacgggccg tggccatcgc gctgagaaac
     3061 cgctggcggc ggatgcagct cgaagaggag atgcgcgagg agatatcgcc caagaacatc
     3121 ctgatgatcg ggcccaccgg ggtgggcaaa accgagattg cgcggcggct cgcccgcctg
     3181 gcgaatgccc cgttcatcaa ggtggaagcc accaagttca ccgaggtggg atacgtgggt
     3241 cgggatgtcg agtcgatcat ccgggatctg gcagacgtgg cggtgaagat gctgcgcgag
     3301 gagcagatgg accgcgtcat gcaccaggcc gaggatcggg ccgaagagcg catcctggac
     3361 gcgctgctcc ccggaccgaa gcccaccggc tctcagacca tcggcttcac ccaggaatcc
     3421 tcggaggacg aaggggactc caccacgcgt cagctcttcc gcaagcgcct gcgacagggc
     3481 gagctcgacg aaaaggaaat cgagatcgag acggatgcgg cccaggtcgg cgtggagatc
     3541 atggcgcctc ccggcatgga ggagatgacc agccagctgc agagcatgtt cagcaacctc
     3601 ggcggcggca gaaaacgctc cgtgcgcctg aaaatcaagg acgcgttgcg caagctcaag
     3661 gaagaggaag cggcgaagct ggtaaacgag gacgagatca aggcacgcgc cgtggaaacc
     3721 gtggagcaga tgggcatcgt gttcctggac gagctggaca aggtggcaaa gcgcagcgag
     3781 agcgcgggcg cggacgtctc ccgggagggc gtgcagcgcg acctgctccc cctgatcgag
     3841 ggctgcacgg tgtccaccaa gtacggcatg gtgaaaaccg atcacatcct cttcatcgcc
     3901 agcggcgcgt ttcacctggc gaaaccttcg gatctgatcc ccgagctgca gggccggctg
     3961 cccatccgcg tagagcttgc ggccctgtct gccggcgact tcaagcgaat cctggtcgag
     4021 cccaacgcat cgctcaccga gcagtatcag gcgctgctgg ggatcgatgg gctcagcctc
     4081 gaattcaccg acgatggcat cgagcgcatc gccgagctgg cgtggcaggt caacgagagc
     4141 accgagaaca tcggcgcgcg gcgcctgcac acggtcctgg agcgcctcct ggaaaacgtt
     4201 tcgttcacgg ctaccgagag cgcggaaagc acggtggtca tcgacgcggt ctacgtggac
     4261 gctcagctct cggagctggc ccaggaccgc gatctttcca gatacatcct gtaggcgacg
     4321 ggccaaggcc atgcagcgcc caaagcagat tacctatcgc cgccagtcaa aagtcctggt
     4381 tctctctttc gaggacggcg acgcggagat ccccgcggag ctgctgcgcg tctactcacc
     4441 ctctgcggag gtgcggggcc actcggccgc agagcgcaag ctgcagacgg gcaagaagca
     4501 cgtcggcatc aaaagcctgg agcccgtagg taattacgcg atccgcatta ccttcgatga
     4561 cggccacgac accggtctgt acggttggga ccttctgcgg gatctcgccg agaacgccga
     4621 cggttactgg aagtcctatc tcgacgagct gaaggcggcc aacgcctcgc ggcttcccac
     4681 cataccggtg ggccactgga cgccggcaag ccagccggcc gtccccccgg agcaggatca
     4741 atgagcgatc agaactccgt cgacttcggc tcccgcaagg tatcgccaga tgagaagacc
     4801 cgcctggtag gcggagtttt cgcgagcgtg gctgaacgct acgacgtcat gaacgacctg
     4861 atctctctgg gcacccaccg cctgttcaag cggatgacgg tggagctctc gggagtccgg
     4921 gagggcatgc gcgtgctgga cctggccggc ggcaccggcg atctgagcgc gttgttctct
     4981 ggtgcggttg gcccgtcggg agttgtcgtg ctcgcggaca tcaacgcacg catgatggac
     5041 gtgggccgca accgtctgct ggaccagggt tgcgcaaacg tctcgttctg ccaggcagcg
     5101 gcggaagctc tgcccttcgc cgacgacagc ttcgactgcg tgagcatcgg gttcggattg
     5161 cgtaacttca ccgacaaacg ggttgcgctc gaggagctca atcgcgtgat cgcgccgggc
     5221 ggcgtgctgc tgatcctgga gttctccagg ccggagaacg catttctgga gggggcctac
     5281 gggacgtttc agtcgctctg gcccggactc ggcaagctgg tggctggcga cgcccgaagc
     5341 taccagtacc tcatcgagtc gattcgcaat catcccccgc agaaggcgct caagctcatg
     5401 ctcgaagacg ccggcttcgc cgccgtcgag tatcacaatc tgctgggggg catcgcggcc
     5461 attcaccgcg gggtcaagcc atgagcaccg cagacgtttt cgaatcagcc ctggtggagc
     5521 tcgtcaacag ggcggccaac gcaacgctcg agctggaccc caaagccgca tcccggctgg
     5581 ccgcggcgga aggacaccgg gcgcgcctca tcgcagcgct gcccggcggc cgtgagcaat
     5641 ccttcacgct gtcggtcgtc ggggggcgca tggcgttcac gccgcacgcc gaggccgagc
     5701 ccaacgtagt cgtgcgcggt tccgttccgg ctctgctttc catgctcgcc ggaggcagaa
     5761 gcgccgggcc ggtcaccatc gaaggcgacg tcagcgtgct ggaggagctg gcggccatct
     5821 tcaagggctt cgcacccgac ctggccgagc cgctgtcgaa gctggttgga gaaacctcag
     5881 cagacggcat tctgaacgcg gcggaggcag gtctggccct gctgaaatct gcagcagagg
     5941 ctacaggcga atccctgcgg cgcggcgtgg gcgagcgttt tgcgacgcgc acccgcgtca
     6001 acgttcttct cgatcggctg gaagatgccc gcgaacgatc ggaccgcctc gccgcacgca
     6061 taggcaatct ggagagcgcc cggggctccc accagcgccc ggaacctcca acgccatgag
     6121 cgctctgcgc cgactctttc gggttctctg gatcgtgagc cgctaccggc tcgacaccct
     6181 gatcgacgcg gatcaggaag gcgcggcgtt gccgcgtcgc tacgcctggc tactgcgcgc
     6241 ttctcccgcc cggcttctac cggtcggcga cgaaccgcgc gggcggcgcc tgctgctggc
     6301 gctggagcag ctcgggccgg tgttcgtcaa attcggacag ctgctttcca cgcggcggga
     6361 tctgataccg ctggacattg cggacgagct cagcgccctg caggatcggg tgcccccgtt
     6421 tcccggcgag caggccgtgc ggatcatcga ggacgcgctg ggccaaccca tcagcgaagc
     6481 cttccgctcc ttcgagatag agcccatggc atccgcgtct gtggcccagg tgcatgccgc
     6541 catcctgccg gacggcaaga gcgtggtggt caaggtcgtc agacccgaca tagccgacgt
     6601 catccgggca gatctggaac tgctgcgcgc cgccgcggag attctggaga acgcctctga
     6661 ggaggcgcgg cggctgcacc tcaagcaggt ggtcaaggac tacgagcaaa cgattctcgg
     6721 tgagctcgac atgcagaagg aggcggacaa caccgcccgg ctgcgcagca acttcgccga
     6781 gtcagagctc ctgtacg
//
LOCUS       DBNHQR010000071         2569 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_260433, whole genome shotgun sequence.
ACCESSION   DBNHQR010000071 DBNHQR010000000
VERSION     DBNHQR010000071.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_260433"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1103)
                     /locus_tag="AC16VR_01565"
     CDS             complement(<1..1103)
                     /locus_tag="AC16VR_01565"
                     /inference="COORDINATES: protein motif:HMM:NF013225.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HIR6009867.1"
                     /translation="MSWETEVGEIERRRHLARQQGGPEGIARQHAKGRLTIRERIGEL
                     LDEGSFREQGKATAIPDYDENDELTGFVPANYVVGFGQVEGRRVVVGGEDFTRKGGSP
                     NAAGLRKSVYAEHLASEYRVPLIRLLEGGGGSVKGSGRKGGTVGEPVYADPRFRIIAD
                     TMTEVPVASGAMGAVAGFPAGRLVASHFSVMTRHTAQVLIGGPALVERALGVRLSKEE
                     LGGVQVHALSGVVDNIAEDEHDAFRQIRRFLSYLPGNVWQRAPRAPSDDDPTRMEQEL
                     LSAVPRDSNAPFDMRVIVEMIVDLGSFFEIGATFGPSQICGLARLDGQPVGVLANDNR
                     HYAGAMTAQAAQKYRRFVEMCDTFHLPIVNFVD"
     gene            complement(1116..1919)
                     /locus_tag="AC16VR_01570"
     CDS             complement(1116..1919)
                     /locus_tag="AC16VR_01570"
                     /inference="COORDINATES: protein motif:HMM:NF005699.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HIR6009868.1"
                     /translation="MSELVTIDVSGGVADVRLNRPEKYNALSPQMFDAIVAAGERLAQ
                     AKDVRAVVLSGNGRGFCAGLDMASFQGMTETSPRSSAGSLLQQDDRPENRAQRPALVW
                     KRLPVPVIAAIHGVAYGGGCQIALGADIRFAAPDAKMSIMEIKWGLIPDMSITQTLRD
                     VLPLDLAKELTFTGRVLSGAEASQLGLVTHVVEDPLAAALELAREIAGKSPHAVRAGK
                     KLFEESWHADARTGLELEAALQTGLIGSPNQVEAIKANFENRKPDFRDD"
     gene            complement(1976..2200)
                     /locus_tag="AC16VR_01575"
     CDS             complement(1976..2200)
                     /locus_tag="AC16VR_01575"
                     /inference="COORDINATES: protein motif:HMM:NF012584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016421 - CoA carboxylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HIR6009869.1"
                     /translation="MARYEVESEVQGNVWKIEVGEGDRVSAGDVLIILESMKMEIPVE
                     APVSGVVAELRVREEEAVEEEQILLVLEAD"
     gene            complement(2210..>2569)
                     /locus_tag="AC16VR_01580"
     CDS             complement(2210..>2569)
                     /locus_tag="AC16VR_01580"
                     /inference="COORDINATES: protein motif:HMM:NF014804.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009870.1"
                     /translation="AEDPRTLLPDTGVLRVFRAPRMHRVRVETGYQEGQAVTPFYDAM
                     LAKVIGWGETRELAIGRTLVGLKGFEIRGVKTNIPLLLDVLQDDGFLAGLIHTGFLDG
                     LVLGSDLRSGGGVGRNA"
BASE COUNT          440 a          925 c          756 g          448 t
ORIGIN      
        1 tggtccacga agttcacgat gggcagatga aaggtgtcgc acatctcgac aaagcgacga
       61 tacttctgcg ccgcctgggc cgtcatcgcg ccggcatagt gccgattgtc gttggccagc
      121 acccccacgg gttggccgtc cagcctggcc agcccgcaga tctgacttgg cccgaaggtc
      181 gccccgatct cgaaaaaact gcccaggtcc acgatcatct cgacgatgac ccgcatgtcg
      241 aagggcgcgt tggaatcccg cggcacggcg gaaagcagct cctgctccat gcgggtcgga
      301 tcgtcgtcgc tcggcgcgcg cggagcgcgc tgccagacat taccgggcag gtaactcaga
      361 aagcggcgga tctggcgaaa ggcgtcgtgc tcgtcctccg cgatgttgtc caccacgccg
      421 ctcagcgcat ggacctgcac gccgccgagc tcttccttgg acaggcgcac gcccagcgcc
      481 cgctcgacca gggccggacc gccgatgagc acctgagccg tgtgccgggt catcaccgag
      541 aagtgggacg ccaccagccg gccggccgga aatccggcga ccgcccccat ggcccccgat
      601 gccaccggaa cctcggtcat cgtgtcggcg atgattctga agcgcgggtc ggcgtacacg
      661 ggttcaccga cggttcctcc tttacgaccc gatcccttca cgctgccccc gccaccctcg
      721 aggagccgga tcagcggcac gcggtactcg ctggcaaggt gctcggcgta gacgctcttg
      781 cgcaatccgg ccgcgttcgg agagccgccc ttgcgggtga agtcctcgcc gcccaccaca
      841 acgcggcgtc cctcgacctg gccgaaaccc accacgtagt tggccggaac gaacccggtc
      901 agctcgtcgt tctcgtcgta gtcgggtatg gccgtcgcct tgccctgctc gcgaaaactg
      961 ccctcgtcca gaagctcgcc gatccgttca cgaatggtga gccggccctt ggcgtgctga
     1021 cgtgcaatgc cctccggccc gccctgctgg cgcgcaagat ggcggcggcg ttcgatctca
     1081 ccaacctcgg tttcccagct catcgtgcgg ttccgtcaat cgtccctgaa atcgggcttc
     1141 ctgttctcga agttcgcctt gatggcttcg acctggttgg gcgatccgat cagacccgtc
     1201 tgcagggccg cctccagctc cagccccgtg cgcgcatccg cgtgccagct ctcctcgaac
     1261 agcttcttcc cggctcgaac ggcatggggc gacttcccgg cgatctcacg ggcgagctcc
     1321 agggcggcgg ccagggggtc ctccacgacg tgagtcacca gcccgagctg cgaggcttcc
     1381 gctccgctga gcacacgccc ggtaaaggtc agctccttgg cgagatctag aggcagaacg
     1441 tcacgcaggg tctgggtaat gctcatgtcc ggaatgaggc cccatttgat ctccatgatg
     1501 gacatctttg catcaggagc cgcgaagcga atgtcagcac ccagcgcgat ctgacatccg
     1561 ccgccgtagg cgacgccgtg tatggcggcg atgacgggca ccggcagacg cttccagacg
     1621 agagccggcc gctgggcccg gttttcgggt cggtcgtctt gctgcagcag gctgcccgcg
     1681 gatgaccggg ggctggtctc ggtcatgccc tggaagctgg ccatgtcgag accggcacag
     1741 aaaccgcggc cgttgccgga cagaaccacc gcgcgcacgt ccttagcctg tgccagccgc
     1801 tctccggctg cgacgatggc atcgaacatc tgcgggctca gcgcgttgta cttttccggc
     1861 cggttgagac gcacgtcggc cacgccgccc gatacgtcga tggtgaccag ttccgacata
     1921 gtttcccccc aacccagaag acggccctct aacttaacac aattgctcgt ccgccctagt
     1981 ccgcctcgag caccagcaga atctgttcct cttcaacagc ctcctcctcg cgcacgcgca
     2041 gctccgcgac gacgccggag acaggcgcct ccaccggaat ctccattttc atggactcca
     2101 gaatgatcag cacgtctccc gccgacaccc ggtcgccttc gccgacctcg atcttccaga
     2161 cgttaccctg aacctcactt tcgacctcgt agcgagccac gttacctcct catgcgttcc
     2221 ggccgactcc gcccccgctc cgcagatcgg agccaagcac aagcccatcg agaaatccgg
     2281 tgtggataag cccggcaaga aacccgtcgt cctgcagcac gtcgagcagc agcggtatgt
     2341 ttgtcttgac cccccggatc tcgaatccct tgagcccaac cagcgttcgg ccgatggcaa
     2401 gctcgcgggt ctcgccccag ccgatgacct ttgccagcat ggcatcgtag aacggcgtaa
     2461 cggcctgacc ctcctggtaa cccgtctcca cccgtacccg gtgcatccgc ggcgcccgga
     2521 aaacgcgcag aacgcccgtg tccggcagga gcgtgcgcgg atcttccgc
//
LOCUS       DBNHQR010000072         3156 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1654229, whole genome shotgun sequence.
ACCESSION   DBNHQR010000072 DBNHQR010000000
VERSION     DBNHQR010000072.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1654229"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="AC16VR_01585"
     CDS             <1..672
                     /locus_tag="AC16VR_01585"
                     /inference="COORDINATES: protein motif:HMM:NF013621.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase family protein"
                     /protein_id="HIR6009871.1"
                     /translation="ASEERDLVELIWHALSRTLELHLESAPPDTFDGGLTSALEHWRA
                     LVLGEETAHATAPHDGRLLLPGSFNPLHRAHRRMLELAEHLTGLEGAYELSIVNVDKP
                     LLDYTEIEERLSQFEAPVWLTRLPTFVDKARYFPGAHFAVGVDTIIRITEAGYYGSQA
                     DRDAALDELATLGTRFLVFGRILNDAFLTLSDLDLPATFRALCREVTEAEFNEPISST
                     EIRRR"
     gene            complement(687..1577)
                     /locus_tag="AC16VR_01590"
     CDS             complement(687..1577)
                     /locus_tag="AC16VR_01590"
                     /EC_number="3.2.2.-"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF018533.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fpg/Nei family DNA glycosylase"
                     /protein_id="HIR6009872.1"
                     /translation="MPELPDLTVYVEHLERRLVGQPLEAIRIASPFVLRSVEPTVDEV
                     CGRRILGCHRLAKQLVMELEGEHFVVVHLMIAGRLKWQKPSAPLPKPNALAAFDVPAG
                     SLLLTEASSKKRASLRLVRGAHALGALNPGGLEVLSTDLAAFASRLRESNHTLKRALT
                     DQHVFAGIGNAYSDEILLRARLSPFKRPRDMADGEIQRLHEATKGVLGEWTDRLRAAA
                     GDDFPSKVTAFHPEMSVHGKYRQPCPVCGAPVQRIVYAENEANYCARCQTGGRLLADR
                     SLSRLLKENWPKRVEELEGG"
     gene            complement(1570..2949)
                     /locus_tag="AC16VR_01595"
     CDS             complement(1570..2949)
                     /locus_tag="AC16VR_01595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR6009873.1"
                     /translation="MSEGGQAGELQGRRPGIWELAFPSILSNLSFTLVGLVQTKFVGE
                     LGAQALAAVGAGQRVFFAMQAILMAVSAGTTALVARAWGAGDYVEASRVTMASLVLAG
                     AFSVVVAVIGLFFAPVIAGVFGLDPETLGMASENIRWLCVFNVAFAVNFILGAALRAS
                     GDAWSPLWISGAVNVVNVPLLYVFIFGEFGFPRMGVAGAAIAAGIAFSIGGAVLLGLW
                     VKQKFRVKHVASGWWRKARLRQLLDIGYPAAVEQGVFQLGFFIFLMLIGNFYGTEAFA
                     AYNIGANMLMVCMTVGFGFSIAGATLVGQHLGANDPDGAARSGWRSTGLAVLAMGGLG
                     GVVCFYAEELAVFFIGDEPLTVDYTVQFTYILGAMMPLLAVEFTIGGALRGAGDTRFP
                     LMATFLGLIGMRCGLAALATYLGVPVFWVYAALIGDYLLKGVLLISRFRGGRWKTAIR
                     YREPEALGA"
BASE COUNT          601 a         1071 c         1011 g          473 t
ORIGIN      
        1 gccagcgagg agcgcgatct ggtggagctc atctggcacg cgctcagccg gacgctagag
       61 ctgcacctgg agtcggcacc gcccgatact ttcgacggcg gtctgaccag cgcgctggaa
      121 cactggcgcg cgctggtgct cggtgaggag accgcccacg ccaccgcacc gcacgacggc
      181 cgtctgctgc tgcccggctc gttcaatccg ctccatcggg cgcaccgccg gatgctcgag
      241 ctggccgagc atctcaccgg cctcgagggg gcatacgagc tgtcgatcgt gaacgtggac
      301 aaaccgctgc tggactacac ggaaatcgag gagcggctca gccagttcga ggcgcccgtc
      361 tggctgaccc gactgccgac tttcgtcgac aaggcccgtt acttcccggg cgctcatttt
      421 gccgtcggcg tggacacgat catccgcatc accgaagccg gctactacgg cagccaggcc
      481 gaccgggacg cggcccttga cgagcttgcc actctcggta cgcgctttct cgtcttcggc
      541 agaattctga acgacgcctt cctgacgctc tccgatctgg acctgccggc gacgttccgg
      601 gcgctgtgcc gcgaggtgac cgaggcggag ttcaacgagc cgatctcctc aacggagatc
      661 cggcgacgct agcccaacgg ggccgctcag ccgccctcga gctcttccac ccgcttcggc
      721 cagttctcct tgagcaggcg ggacagggac cggtccgcca gcaggcgccc gcccgtctgg
      781 catcgagcgc agtagttggc ctcgttctcg gcatacacga tgcgctgcac gggagcgccg
      841 caaacgggac acggctggcg atactttccg tgcacggaca tctccggatg gaaagcggtc
      901 accttcgacg ggaagtcgtc cccggccgcc gcccggagac ggtccgtcca ctcgccaagc
      961 acgcccttgg ttgcctcatg gagccgctgg atctcaccgt ccgccatgtc ccgcgggcgc
     1021 ttgaacggcg acagccttgc ccgcagcagg atctcgtcgg agtaggcgtt gccgatgccc
     1081 gcaaagacgt gctgatccgt cagagcccgc ttcagcgtgt ggttggattc gcgcagtcga
     1141 gacgcaaagg cggcaagatc ggtcgagagg acctcgagac cgcccggatt cagcgctcca
     1201 agggcgtggg cgccgcgcac cagccgcagc gacgcccgct tcttgctgct ggcttccgtg
     1261 agcagcaggc tgcccgcggg aacgtcgaag gcggccagcg cgttgggttt tggcaggggc
     1321 gcgctcggct tctgccactt cagccggccc gcgatcatga ggtgaacgac cacgaagtgc
     1381 tcgccctcca gctccatgac cagctgcttg gcgagccgat ggcaacccaa gatacgccgg
     1441 ccgcagacct cgtccaccgt cggttcgacc gagcggagca cgaaagggct ggcaatgcgg
     1501 atggcctcga gcggctggcc caccagtcgc ctctcgagat gctcgacgta gacggtcagg
     1561 tcgggcagct caggcaccga gggcctccgg ttcccggtag cggatggccg ttttccagcg
     1621 gccgccgcgg aaccgcgaga tgagcaggac gcccttgagc aggtagtcgc cgatcagtgc
     1681 tgcgtacacc cagaacacgg gcacgccgag ataggtggcc agggccgcga gcccgcagcg
     1741 catgccgatg agcccgagaa aggtcgccat gagcgggaag cgggtgtcgc cagcacccct
     1801 gagcgcgccg ccgatggtga actcgacggc cagcagcggc atcatggcgc cgaggatgta
     1861 ggtgaactgc accgtgtagt ccacggtaag gggctcgtca cctatgaaaa agacggccag
     1921 ctcctctgca tagaagcaga cgaccccccc caagccaccc atggcgagca ccgcaaggcc
     1981 cgtggagcgc cagccgctgc gggcggcgcc gtccggatcg ttcgcgccga ggtgctggcc
     2041 caccagcgtc gcgccggcga tggagaagcc gaacccgacg gtcatgcaca ccatgagcat
     2101 gttggcgccg atgttgtagg cggcgaaagc ttcggtaccg tagaagtttc cgatcagcat
     2161 caggaagatg aagaagccga gctggaacac cccctgctcg accgctgccg ggtagccgat
     2221 gtcgagaagc tggcgcaacc gcgccttgcg ccaccagccg ctcgccacgt gcttgacccg
     2281 gaacttctgt ttgacccaga gcccgagaag gacggccccg ccgatggaga acgcgatgcc
     2341 ggccgcaatg gctgctccgg caacccccat gcggggaaaa ccgaactcgc cgaagatgaa
     2401 cacgtagagc agcggaacgt tgacgacgtt gaccgcgccg ctgatccaaa gaggcgacca
     2461 ggcatctcca gatgcgcgca gcgcggcgcc caggatgaag ttgacggcga aggcgacgtt
     2521 gaaaacacac agccagcgga tgttctctga ggccatgcca agcgtttccg gatcgagccc
     2581 gaagacgccg gcaatgacag gggcgaaaaa cagaccgatc accgcgacca cgaccgagaa
     2641 ggcgccggcg agaaccagcg aggccatggt gacgcggctc gcctcgacgt agtccccggc
     2701 gccccaggct ctggccacta gagccgtggt gcccgcgctg accgccatca gaatggcctg
     2761 catggcaaag aagacacgct gccctgcgcc cacggccgcg agcgcctggg cacccagctc
     2821 gccgacgaac ttggtctgca caagaccaac gagggtgaat gacaggttgc tcaggatgga
     2881 cggaaacgcc agttcccaga tgcccggtcg ccgtccctga agttctcctg cctgaccgcc
     2941 ttcggacatc tgcctccttc tcgaacgcac caggctgctt atggaatgcc cgccgcgctg
     3001 gctgctaata tcctccgctc gctcgaattc agcaaacggg gataaggatc ttgactgaat
     3061 acaccgacat cggcgtgacc cgggacggcc acgtcactac cgtggagatc cagcgtccgc
     3121 caaacaattt cttcgatcac agcctgatca ggcaga
//
LOCUS       DBNHQR010000073         2318 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_957646, whole genome shotgun sequence.
ACCESSION   DBNHQR010000073 DBNHQR010000000
VERSION     DBNHQR010000073.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_957646"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..937
                     /locus_tag="AC16VR_01600"
     CDS             161..937
                     /locus_tag="AC16VR_01600"
                     /inference="COORDINATES: protein motif:HMM:NF018777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD domain-containing protein"
                     /protein_id="HIR6009874.1"
                     /translation="MIRITFLLRRKPELSAEEFYRYWREEHGPLVASHASRLNTLRYV
                     QVHTIDDPANQAMATARGGMEAPYDGVAEMWWESRAALVASLETKGGRRAGAELVEDE
                     ARFIDLPNSPMWLGHEYPQVNPTPENLVARERDTLVKLYFPLRPVQGLDERDAQSYWY
                     SNHGPIIRRQAAGVGIQRYVQVHRVADELEAGLRKARGTQVEGYMGHAELWFDRTVLG
                     VSTPERRAANERAIADESNFIDFRRSCMWLAKEHVFVDRR"
     gene            complement(954..2246)
                     /locus_tag="AC16VR_01605"
     CDS             complement(954..2246)
                     /locus_tag="AC16VR_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6009875.1"
                     /translation="MAWDFSTEPEFQKELDWMDEFVREEIEPLDFIIGHPNDFSDPNR
                     AKLIPPLQEEVKKRGLWACHLGPELGGQGYGQVKLGLMNEILGRAKCAPVVFGCQAPD
                     SGNAEILAHYGTPEQKDRYLEPLLNNEICSCYSMTEPQGGADPKVFTCRAELIGDEWV
                     INGEKWFSSNARYASFLIVMAVTDPEAPPYQRMSMFIVPTETAGIEIVRHVAVGYESK
                     GAHAYIRYNDVRIPRDHMLGEPGQGFIVSQVRLGGGRIHHAMRTMGKARRAFDMMCER
                     ALSRETQGEVLAQKQLVQAMIADSWIDIEQFRLFVLRTAWKIDKLKNYKEVRADISAV
                     KAQMPKVYHDVYARALQVHGSLGLSTEMPFAAGVLDSFHMGLADGPTEVHKVTVAKQV
                     LGQYQGTDDLFPEYHLIRRKEAALAKYADIIEHEVGNA"
BASE COUNT          374 a          747 c          782 g          415 t
ORIGIN      
        1 gcttcccgct gcggtcgcca tgatctggct gccgcaaccc ggaaccgaca cggcgtcctg
       61 acccgtgcgc atgcatttcg gccggtgccg gttgcatgtg agatcccccg cggtttaatt
      121 tcagcggcca ggggccgtcg aaatttttct tgggggaagc atgattcgca tcacttttct
      181 gctgcgcagg aagccggagc tgtcggccga ggagttctat cgttactggc gcgaggagca
      241 cggcccgctg gtggcgagtc acgcaagccg gctgaacacc ctgcgctacg tccaggtcca
      301 caccatcgac gaccccgcca atcaggccat ggccaccgcc cggggcggca tggaagcgcc
      361 gtacgacggc gttgccgaga tgtggtggga gagccgtgcg gcgctcgtgg cctctctgga
      421 aaccaaaggc ggccggcggg cgggcgccga gctggtggag gacgaggcgc gtttcatcga
      481 tctgcccaac tcgcccatgt ggctcggcca cgagtacccc caggtgaacc cgacgccgga
      541 gaatctggtc gcccgggagc gcgacaccct ggtgaagctg tacttcccgc tacgaccggt
      601 tcagggcctg gacgagcgcg acgcccagag ttactggtac agcaaccacg ggcccatcat
      661 ccggcgacag gccgccggtg tcggcatcca gcgctacgtt caggtccacc gggtggcgga
      721 cgagctggag gccgggctgc gcaaagccag gggtacccag gtggagggtt acatgggcca
      781 cgccgagctg tggttcgacc gcaccgtgct cggcgtttcc acgccggagc ggcgggcggc
      841 caacgagcgc gccatcgccg acgagagcaa cttcatcgac ttccggcgct cgtgcatgtg
      901 gctggccaag gagcacgtct tcgtcgaccg ccggtgacgg cgacgcgggt ggctcaggcg
      961 ttgcccacct cgtgctcgat gatgtccgcg tacttggcca gggccgcttc cttgcgcctg
     1021 atcaggtgat attccgggaa caggtcgtcg gtaccctggt actgcccgag cacctgcttc
     1081 gccacggtca ccttgtgaac ctcggtgggc ccgtcggcca gccccatgtg gaacgagtcc
     1141 agcacgccgg cagcaaaggg catctccgtg gacaggccca gggagccgtg cacctgcagc
     1201 gcgcgggcat agacgtcgtg gtagaccttg ggcatctggg ccttgacggc tgagatgtcc
     1261 gcgcggactt ccttgtagtt cttgagcttg tcgatcttcc acgcggtgcg cagcacgaac
     1321 aggcggaact gctcgatgtc gatccaggag tccgcgatca tggcctggac gagctgcttc
     1381 tgcgccagca cttccccctg ggtttcccgc gacagggccc gctcgcacat catgtcgaaa
     1441 gcgcggcgcg ccttgcccat ggtgcgcatg gcgtggtgaa tgcggccgcc gccgagccgc
     1501 acctgagaga cgatgaatcc ctggcccggc tccccgagca tgtggtcccg ggggatgcgc
     1561 acgtcgttgt agcggatgta ggcgtgggcc cccttggact cgtagcccac ggccacgtgg
     1621 cggacgatct cgatgccggc cgtctccgtg ggaacgatga acatggacat gcgctgatag
     1681 gggggtgcct ccgggtcggt caccgccatc acgatgagga acgacgcgta gcgggcgttg
     1741 gaggaaaacc acttctcccc gttgatcacc cactcgtcac cgatgagctc cgcgcggcag
     1801 gtgaacacct tgggatcggc gccgccctga ggttccgtca tggagtaaca ggagcagatc
     1861 tcgttgttca gaagcggctc caggtagcgg tccttctgct ccggcgtgcc gtagtgggca
     1921 aggatctccg cgttgcccga gtccggcgcc tggcagccga agaccacggg tgcgcatttg
     1981 gcccggccga ggatctcgtt catcagcccc agcttcacct ggccgtagcc ctggccgccg
     2041 agctcggggc ccagatggca ggcccacagg ccgcgcttct tcacctcttc ctgcaggggc
     2101 ggaatgagct tggcccggtt ggggtcggag aagtcgttgg ggtggccgat gatgaagtcg
     2161 agcggttcga tctcctcgcg gacgaactcg tccatccagt ccagctcttt ctgaaactcg
     2221 ggctcggtcg agaaatccca tgccatggcg tgcgtcctcc ctggtgaaga aggcgagctt
     2281 accagaggcg tctttgcagg tcgttctccg aggtcgtc
//
LOCUS       DBNHQR010000074         2324 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_387004, whole genome shotgun sequence.
ACCESSION   DBNHQR010000074 DBNHQR010000000
VERSION     DBNHQR010000074.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_387004"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="AC16VR_01610"
     CDS             complement(<1..195)
                     /locus_tag="AC16VR_01610"
                     /inference="COORDINATES: protein motif:HMM:NF012880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR6009876.1"
                     /translation="MDLPEEQMIRMLETMVRIREFDERAALLMEQARIHGSVHLYCGE
                     EAVGVGVCEALNPDDYITSTH"
     gene            complement(277..1164)
                     /locus_tag="AC16VR_01615"
     CDS             complement(277..1164)
                     /locus_tag="AC16VR_01615"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6009877.1"
                     /translation="MRLGFSIPNNQGVERVSDLVRLAVEAEELGYASVWVSEHLWHAG
                     YVARRLGEKPYHEPLTVLTAIAAATRQVRLGTSVLVLPWHHPVRLAKTIATLDDLSDG
                     RVSLGVGVAVTEDEFENLGVDFATRGRRADDALAAIKALWTQEFPRHEGPFYQFSGMR
                     FEPKPRQSPHPPILVGGGAPPALRRAARFADGWHALGQSPESLAESMKMLEESCRAVG
                     RDVADLDISIRCVIEIIGEPWDRPVTRRRTLKGTEREIAETIAAYAEAGVDEIVLDAN
                     LTDLEATRAVMHRGIRFQD"
     gene            1300..>2324
                     /locus_tag="AC16VR_01620"
     CDS             1300..>2324
                     /locus_tag="AC16VR_01620"
                     /inference="COORDINATES: protein motif:HMM:NF047238.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase C-terminal domain-containing protein"
                     /protein_id="HIR6009878.1"
                     /translation="MQADIDATAGRHLRGRYAVVGVGETGYVRGSGCTTRALGTRAVR
                     AALADAGLEPGDVDGMLSYSYNDSTFSPFIAGDLGIRLNFYMDVYGGGSSIEALIGIA
                     MGVIEAGMCRTVVIFRAMNGYSQVRIGGTGGRGAVAPLGGDALFNRAFGQMSAGQMFC
                     QSFMRHMYDYGTTPEQVAMVKVVHSEHASNNPKAYYRQRVSVDDVVNSRMIVKPLHLL
                     DCCVETDNGSALIVTTTERARDLKQTPAIIMGVAGRTSKEQAGYHWTRGPISRVAGHY
                     AAPIVFGQAGIGPEDVDVTGSYDAFTFTSMLQLEAYGYCAEGEGGDYVSSGIIELGGV
                     RPNNTSGG"
BASE COUNT          392 a          781 c          781 g          370 t
ORIGIN      
        1 gtgtgtcgag gtgatgtagt cgtccgggtt cagcgcttcg cagacgccga cgccaacggc
       61 ctcctccccg caatacagat gcaccgagcc gtggatgcgg gcctgctcca tgagcagcgc
      121 agcccgctcg tcgaactcgc ggatccggac catggtctcg agcatgcgaa tcatctgctc
      181 ttcgggaaga tccatggcag cgctcctgag aataagacat cgaaggctaa cgacgcccgc
      241 caaccaggtc aaacagcgcc gcaaggagcg gccggctcag tcctggaacc ggattccccg
      301 gtgcatgacg gcgcgggtag cctccaggtc ggtcaggttc gcatcgagaa cgatctcgtc
      361 cacgcccgcc tcggcgtagg cggcgatggt ctccgcgatc tctctctccg tgcctttcag
      421 cgtccggcgc cgagtcaccg gtcgatccca gggttcccct atgatctcga tgacgcagcg
      481 aatgctgatg tcgagatccg ccacgtcccg acccaccgcg cggcacgatt cctccagcat
      541 tttcatcgac tccgccaggg actccgggct ctgcccgagc gcgtgccagc cgtctgcgaa
      601 ccgcgcggcg cgccggagtg cgggcggcgc gccgccgccc accaggatgg gcgggtgcgg
      661 cgattgccgt ggtttcggct cgaaacgcat cccggagaac tgatagaagg ggccttcgtg
      721 gcggggaaac tcctgggtcc agagcgcctt gatggcggcc agcgcgtcgt ccgcgcgccg
      781 gccccgggta gcgaagtcga cgccgaggtt ctcgaactcg tcctcggtga ctgccacccc
      841 gacccccagg ctgaccctgc cgtcggagag gtcgtccagc gtggcgatgg tcttcgccag
      901 ccgaaccgga tgatgccagg gcagcacgag cacggacgtg cccaggcgga cctgccgggt
      961 ggcggcggca atggcggtga gcaccgtcag gggttcatga tagggcttct cgcccaggcg
     1021 cctggccacg tagcctgcgt gccagagatg ctcgctcacc cacaccgacg cgtaaccgag
     1081 ctcctccgcc tcaaccgcca gccgcaccag gtcggagacg cgctccacgc cctggttgtt
     1141 cggaatgctg aatccaagcc gcatagctcg aaacgggccc ccagccctgc ctccggtcct
     1201 gcccggattg gttcggcgag tatttcacga tcgccggtac tcccgcacct cgattagtgc
     1261 taaaagaaac ggcaggcagc tttgggagag gagcagccca tgcaggctga catcgacgca
     1321 accgccggac ggcacctgcg cggcaggtac gccgtggtgg gcgtgggcga gaccggttac
     1381 gttcgcgggt ccggctgcac gacccgcgcc ctgggcaccc gcgccgtccg ggccgccctc
     1441 gccgatgcag ggcttgagcc aggcgacgtc gacggcatgc tgtcctacag ctacaacgac
     1501 tcgaccttct ctcccttcat tgccggcgac ctggggatcc gtctgaactt ttacatggac
     1561 gtctacggcg gcggctcgag catcgaggcg ctgatcggga tcgccatggg cgtcatcgag
     1621 gcgggcatgt gtcgcacggt ggtcatcttc cgggccatga acgggtacag ccaggtacgt
     1681 atcggtggca ccggcggccg cggcgcggtg gcgccactcg gcggtgacgc gctgttcaac
     1741 cgggcgttcg gccagatgag cgcggggcag atgttctgcc agtcgttcat gcgccacatg
     1801 tacgactacg gcaccacgcc ggagcaggtc gccatggtca aggtcgttca ctcggagcat
     1861 gcctccaaca accccaaggc ctactacagg cagcgggtga gcgtggacga cgtcgtcaac
     1921 agccgcatga tcgtcaagcc cctgcacctg ctggactgct gcgtggagac ggacaacggc
     1981 agcgcgctga tcgtgacgac gacggagcgg gcgcgcgatc tgaagcagac gccggcgatc
     2041 atcatgggcg tggccggccg cacgagtaag gagcaggcgg gctaccactg gacgcgcggc
     2101 ccgatctcga gggtggccgg gcactacgcc gcgccgatcg tgttcgggca ggcgggcatc
     2161 gggcccgagg acgtcgacgt gaccgggtcg tacgacgcat tcacgttcac ctcgatgctc
     2221 cagctcgagg cctacggcta ctgcgccgag ggcgagggcg gcgattacgt ctctagcggc
     2281 atcatcgagc tgggcggcgt gcggccgaac aatacgagcg gtgg
//
LOCUS       DBNHQR010000075         8353 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1464273, whole genome shotgun sequence.
ACCESSION   DBNHQR010000075 DBNHQR010000000
VERSION     DBNHQR010000075.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8353)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8353)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8353
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1464273"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..753
                     /locus_tag="AC16VR_01625"
     CDS             181..753
                     /locus_tag="AC16VR_01625"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6009879.1"
                     /translation="MSEAEGLGARVAALEEELGRLTDLQAIQDVLVRYSRALDWLDDA
                     MLETVFYDDADIDYGFFRGNGKDFKKALMELEHGIGRRWHFTSQLEIDLNGDSADVES
                     YNLSLALPEVEPSDGGGYSVFFGFYKDRLVKRGGKWGIIARKHIQVSGAPLAELAMEG
                     DMGLLNQIGLTSPSHPDYRRLTNAQPLDDS"
     gene            833..1273
                     /locus_tag="AC16VR_01630"
     CDS             833..1273
                     /locus_tag="AC16VR_01630"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6009880.1"
                     /translation="MKVESLGHLVLKVRDLDRSEQFYSGVLGMPVCARFDDKGLKMTF
                     FSLGNHHDLAIMQVSGEEASAGEQAVGLHHVAFCIGRSMDQLREAKAQLEAAGVETDP
                     IDHDVTKSLYFDDPDGHNIELYVDVSDAWKKEPQRIAVLEPLEL"
     gene            complement(1419..1556)
                     /locus_tag="AC16VR_01635"
     CDS             complement(1419..1556)
                     /locus_tag="AC16VR_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009881.1"
                     /translation="MWAIAITGFAPGSISGLLDGTFVDSQAVHVHAAVCGAHHDAATI
                     D"
     gene            complement(1600..2838)
                     /locus_tag="AC16VR_01640"
     CDS             complement(1600..2838)
                     /locus_tag="AC16VR_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012503.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR6009882.1"
                     /translation="MRTRLPESGSDWPTIKAEMVQLGRGDAKWRDGKTAVYVFNAGDD
                     VAQVQREAYALYMSENGLGPLAFPSLKRMEDEVIDMALSILHAPEDAAGAMTSGGTDS
                     ITMAVKAARNHARAQRGMNAPGNLVIPYSAHPAFDKAAHLMDVQVRRVPVGEDLLADV
                     PAMANAIDRETIMLVGSAPCFPYGLIDDIAALSAVALERGLWLHVDACVGGFIAPFVR
                     MNGSDVPPFDFELPGVASMSADLHKYGYCAKGASTVMFRSDGLYQNMPFDFGDWPGGR
                     MVTPTLAGTRPGGAIAAAWAVMNFLGRTGYQTKHRQVTNARQAVERGVRQLGFEVLGS
                     PKLGIVAFSHAQADVIAVYRHMYRKGWFAGLTTEPKALHLMLSPVHEGVTDLYLTDLA
                     DSLRLAEGQESDDGFEARYS"
     gene            2971..4542
                     /locus_tag="AC16VR_01645"
     CDS             2971..4542
                     /locus_tag="AC16VR_01645"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6009883.1"
                     /translation="MAAVVKPGVRTMDPSFAKLLDSDTNAAHVTRAMRRDSPIQPGPT
                     LVPTARYISREYHELEKQRLWSRVWQMAAHEDDFPNVGDVVPYDIVDRSYLVVRVADD
                     EYKAYYNACLHRGRKLRESRARGLDELKCPFHGWAWELDGTLKQVPCAYDYTDLDREA
                     ERLPEVRIGRWGRFIFVNPDPDCEPLEDFLGDLSSQFEFFPYERRYKQAHVAKIIRAN
                     WKVVQEAFMESYHVYMTHPQALAHGVHDGDTKYDVFGNYSRAILCGALDGSGVPDWGP
                     PPEQNEGITRVRHPLTGTIYESAGDGVVNVTTRDGRSGRFDALANWLDGDLIDANPHM
                     CIVVAGVQLPDIGVAGVETTPEDAARAMERLGDDATGRALMSEMQREVLRRVIPSVAD
                     DVPDIELTAAIYLTVFPNYHPWGSFNRINYRFRPNGDNHEECIMECMYLAPIPDDGDY
                     TPVADIHWLGPDDDWTDAPELGSLAGVFNQDVRNMAFVYDGMKASARECVRLADYNET
                     KIRHFHALYGEWVDG"
     gene            complement(4616..6775)
                     /locus_tag="AC16VR_01650"
     CDS             complement(4616..6775)
                     /locus_tag="AC16VR_01650"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR6009884.1"
                     /translation="MISRLHRLREVALLVVTVLVVPIAHGDDIGELLRPADVRSVIIS
                     PDGRHLAMVRSDDQKDTLVIFARPAMTVATGVASSPGQRFETITWANNRLVLIEPAAD
                     AGAAQQPVPTGAMLALGVDGSRKRLLPRNPEAVRLPTAVISVLPNDLANVLIAAHSAC
                     DAESCSEAEKALRRPVKLNLETGEKTFVAPALAMPAYYVSDPSGNRMLAAGRAADGRV
                     EVYRLAAGSWTPVSSFDPAADLGTVPLAVDRAGSVFALANKVGTAGLYRWNLDSGETD
                     EIYRGAGSDIDRVIMSYGGRRLLAVRSDPGYASWHYLDPEHPFTRIHTALRAAFPDSD
                     VDVTSFTAEGSEAVVRIYSDRNSGDFYSVSLGTGESTLLATSRPWLPAEDLAKVEPME
                     LTSRDMFTLRGYVTTPASAGPHPMIVWIHDKPTTTRAVWELNAEVQMLARRGFAVLQI
                     NYRGSGGLGQWYTASGAERNGQAVQRDIADATRWAIDNGIANEGQICVYGRGYGAFAA
                     VKAMATNIGLFQCAVAIDGFYDLASPFTVPEDPTLTPQNLLTFPSMLDLDDVRRSPVN
                     RVFNIDGSVLLVGESEQTITMRDALEETEKSVVWLAPENEQSGYSEILAYLEAELSAG
                     KAADTDPAGFGATLTARQAREFQRIREEMRAATKELGNRRAFTAAGVRREVEKIIEGY
                     DSDVRSIVTDDQWALYDDFKVGLAQELESELDIVRLQ"
     gene            complement(6768..8306)
                     /locus_tag="AC16VR_01655"
     CDS             complement(6768..8306)
                     /locus_tag="AC16VR_01655"
                     /inference="COORDINATES: protein motif:HMM:NF013729.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HIR6009885.1"
                     /translation="MADADQGFAYEFGDWLVEPDLNRIRRGEQEIQLEPSTLEVLCYL
                     LERPGEIVSVDELLDSVWRDSAAEPSAVHSNISRIRRALGESRREPRYIETVSTDGYR
                     AIGAVRRIEAGPHDAELAEALEAVTPPYPAYNGDEPYTFVCYSHQNREQVYQELVRLR
                     SAGINVWYDEGISPGAEWTEEVANAIANCTHLLFFVSTDSGSSKHCLDELSYAQERHK
                     SIVVVYLEPTELPQGLQLSIGRIQALFKYAMRESEYARKLIATLHGAAETRGASTARP
                     TAPRSPAPPSAGVPGRALRRSLSALGIAALIGALGLLVLRGPRIETILRDEPLENTIT
                     VEAFKDLTAAHDQWIGDTIESEVRVALPGRGLQVVGSASYQGARETSVRERARYRITG
                     TVKRLRSRIRVTAELIANDTDYMIWSEIYDEQITQDAAAQSEIAVRIVADIARKIQGG
                     AEQPLIATIPSARPAAGALQGALDAQPSVPEFSDFDAQEDGDPGQPAFDAFPSFDDDG
                     GGDD"
BASE COUNT         1480 a         2606 c         2751 g         1516 t
ORIGIN      
        1 aggacatgta cgtggctcag cgactgatac cggattgcca ggtgcgcctc atcgagggcg
       61 tggggcactt tctgcatttc gagcggccgg agctgcttga cgactatgcg gagttcatga
      121 ccggctccgc cgtcctgacg agctgaaacc cgtcgagcat ccgcagtcca ggagaagacc
      181 atgagtgaag cagaagggtt gggtgcgcgg gttgctgcgc tcgaagagga acttgggagg
      241 cttacggacc tgcaggccat tcaggacgtg ctggtgcgct actcccgggc gctggactgg
      301 ctggacgatg ccatgctgga gaccgtgttc tacgacgacg cggacatcga ctacggcttt
      361 ttccgcggaa acggaaagga tttcaaaaag gcgctgatgg agctcgagca cgggataggg
      421 cgccgctggc atttcaccag ccagctcgag atcgacctca acggcgattc ggccgacgtc
      481 gagagctaca acctcagcct tgcgcttccg gaggtggaac cgagcgacgg cgggggttac
      541 tcggtcttct tcggcttcta caaggaccgg ctcgtcaagc gcgggggcaa gtggggcatc
      601 atcgcgcgca agcacatcca ggtgagcggc gccccgttgg cggagctggc gatggaaggg
      661 gacatgggcc tgctcaacca gatcggcctg acgagcccct cccaccccga ctatcggcgc
      721 cttacgaacg cccagccgct ggacgattcc tgaccggctt ccaggccgat tcgtgattgg
      781 cttgcacgcg tgggaccggc atagttgcga tgtcgaagtt ctggagctga cgatgaaagt
      841 tgaatctctc gggcacctcg tcctcaaagt tcgcgacctc gaccgctcgg agcaattcta
      901 ctccggcgtg ctcggcatgc cggtttgtgc gcggttcgat gacaagggat tgaagatgac
      961 gttcttcagt ctgggcaatc accatgatct ggcgatcatg caggtatccg gcgaagaagc
     1021 ttcagccggt gaacaggcag tggggctcca tcacgtggcc ttctgcattg gcaggtccat
     1081 ggatcagctg cgtgaagcga aagcgcagct cgaagccgcg ggcgtcgaga cggacccgat
     1141 agaccacgat gtcaccaagt cgctctattt cgatgacccg gacgggcaca acatcgagct
     1201 ctacgtggac gtctcagacg cctggaagaa agaaccgcag cgcatcgccg tgctggagcc
     1261 gctggaactg taaagccggc gggcggccaa ggaagctcat gcgccccact gccgcgacga
     1321 gcagcaacgt cgtggcgacg accatcagcc gcttgtgcgt ttcgggccga ttccgaaaac
     1381 gataggcgac ccagaagagc acgggaaaaa caagcatgtc aatcaatggt ggcagcatca
     1441 tggtgcgcgc cgcagacggc ggcgtgaacg tgcacggcct gcgaatccac gaaggtcccg
     1501 tccagcaggc cggaaatcga tcccggcgca aagcccgtga tggctatggc ccacatccac
     1561 aagctgagcc agaagtaaaa gggccgtgac gggctgagct cagctgtatc gtgcctcgaa
     1621 accatcatcg gattcctggc cttcggcgag ccggaggctg tctgcaagat cggtcaggta
     1681 gagatcggtg accccctcgt ggacgggtga cagcatgagg tgaagagcct tcggctcggt
     1741 cgtcagcccc gcgaaccagc ctttgcggta catgtgtctg tacacggcga tcacgtcggc
     1801 ctgagcatgg gagaaggcaa cgatgccgag cttcggcgag ccgagcactt caaagccgag
     1861 ctgccgcacc ccccgctcga cggcctgccg ggcattggtg acctggcggt gctttgtctg
     1921 atatccggtc ctgccgagga agttcatcac ggcccaggcg gcagcgatgg cgccccccgg
     1981 acgtgtgccc gccagtgtcg gcgtcaccat gcgaccccct ggccagtcgc cgaagtcgaa
     2041 cggcatgttc tggtagaggc cgtcgctgcg aaacatcacg gtggacgcgc ctttcgcgca
     2101 gtagccgtac ttgtgcaggt cggccgacat ggacgcaacg cctggcagct cgaaatcgaa
     2161 agggggaacg tccgagccgt tcatccgcac gaaaggggcg atgaacccgc ccacgcaggc
     2221 atccacgtga agccatagtc cgcgttcgag ggcaacggca gagagcgctg cgatgtcgtc
     2281 gatcagcccg taggggaagc agggggccga gccaacgagc atgatcgtct ctcggtcgat
     2341 ggcgttcgcc atggcgggca cgtcggcaag gagatcctct ccgaccggta ccctgcgcac
     2401 ctgcacgtcc atgaggtgcg ccgccttgtc gaaagccggg tgggccgagt atggaatcac
     2461 caggttgccc ggggcgttca tgccgcgctg agcccgggca tggttccggg cggctttgac
     2521 ggccatggtg atggaatcgg ttccgcctga cgtcatcgcg cccgcagcgt cctcgggtgc
     2581 gtggaggatc gacagggcca tgtcgatcac ctcgtcctcc atccgcttca ggctgggaaa
     2641 tgccagcggt cccaggccgt tctcggacat gtacagggcg taggcttccc tttgaacctg
     2701 ggcgacgtcg tctcccgcgt tgaacacgta aacggccgtc ttgccgtccc gccacttcgc
     2761 atcgccgcgg ccgagctgaa ccatctcggc cttgattgtc ggccagtcgg agcccgattc
     2821 aggtaggcgc gttctcatct gtgctcccct gtccggcggc gcggcgcggc gctgccgctg
     2881 atgcccggag gttaccatct atcagcagac cggtcggcgg gtttggggta gattagggca
     2941 tcgatcaggg taaattgggg gaggagagcc atggcggcag tcgttaaacc cggtgtgcgg
     3001 acgatggacc cgtcgtttgc gaagttgctc gactccgaca ccaacgccgc tcacgtgacc
     3061 cgtgccatgc ggcgcgattc gcccattcag ccgggcccta ccctggtgcc caccgcacgt
     3121 tacatatccc gtgagtatca cgagctggag aagcaacgtc tctggtcgcg cgtgtggcag
     3181 atggccgccc acgaggacga tttcccgaac gtgggcgacg tcgtgcccta cgacatcgtg
     3241 gacaggtcgt acctggtggt tcgggtcgcc gacgatgaat acaaagccta ctacaacgcc
     3301 tgcctgcacc ggggacgcaa gctcagagag tctcgggcaa gagggctcga tgagctcaaa
     3361 tgcccgtttc atggctgggc ctgggagctc gacggcacgc tcaagcaggt tccctgcgcc
     3421 tacgactaca ccgacctcga ccgggaggcg gagcgcctgc ccgaggttcg gatcggacgc
     3481 tgggggcgct tcatcttcgt caatccggat ccggactgcg agcccctgga agacttcctc
     3541 ggcgacctct cgtcgcagtt cgagttcttt ccctacgagc gtcgttacaa gcaggcccac
     3601 gttgccaaga tcatccgcgc gaactggaag gtcgttcagg aggccttcat ggagtcgtat
     3661 cacgtctaca tgacgcaccc ccaggcgctg gctcatggtg ttcatgacgg cgataccaaa
     3721 tacgatgtct tcggcaacta ttcgcgggcc atcctctgcg gagccctgga cggatcaggg
     3781 gttcccgatt gggggccgcc gcccgagcag aacgaaggga tcaccagggt tcgacatccg
     3841 ctcacgggga cgatctacga gtccgccggt gacggcgtgg tgaacgtcac gacccgcgac
     3901 ggccgctcgg gccgattcga tgcgcttgcc aactggctcg atggcgatct gatcgacgcg
     3961 aatccgcaca tgtgcatcgt ggtggccggc gtgcagctgc cggacattgg ggttgcaggc
     4021 gtggagacca cgccggagga tgcggcccgg gccatggagc gactggggga cgacgccacg
     4081 ggtcgtgcgc tcatgagcga aatgcagcgg gaggtgctac gcagggtcat accctccgta
     4141 gccgacgacg ttcccgacat cgagctgacc gcggccatct atctcaccgt gttcccgaac
     4201 tatcatccgt ggggttcttt caaccgcatc aactaccgct ttcgaccgaa cggggacaac
     4261 cacgaggaat gcatcatgga gtgcatgtat ctcgcgccca tacccgatga cggtgactac
     4321 acgccggttg cggacattca ctggctcggt cccgacgacg actggacgga tgcgcccgag
     4381 ctcggcagcc tggccggtgt tttcaatcag gacgtacgca acatggcctt cgtctacgac
     4441 ggcatgaagg catcggcccg ggaatgcgtg cggctggccg attacaacga gacgaagatc
     4501 cgccactttc acgcgcttta cggggaatgg gtggacggct gacgatcctt tccggtcatg
     4561 ccggcttccg ggtcggcccg ctgaatgggg gcgcgaacgg gccgcgagct ccgactcact
     4621 gcagcctgac gatgtccagc tcgctttcca gttcctgggc gagaccgact ttgaagtcgt
     4681 cgtagagggc ccactgatca tcggtgacga tggatcggac gtctgagtcg tagccttcga
     4741 tgatcttctc cacctcccgg cgcacgccgg cggcggtgaa cgcgcggcga tttcccagct
     4801 ccttggtcgc ggctcgcatc tcttcccgga tccgctggaa ctccctggcc tgccgtgcgg
     4861 tcagcgtggc gccgaagccg gccggatccg tgtccgccgc cttgcccgcg ctcagctcgg
     4921 cttcgagata tgcgagtatc tccgagtacc cgctctgctc gttctcgggt gccagccaga
     4981 ccacggactt ttcggtctct tccagcgcat cgcgcatggt gatcgtctgt tcggattcgc
     5041 cgacgagcag aaccgagccg tcgatgttga aaacgcgatt gaccggagag cgcctgacat
     5101 cgtccagatc gagcatcgag gggaatgtca gcaggttctg gggcgtcagc gtggggtctt
     5161 ccgggacggt gaagggcgat gccagatcat agaacccgtc gatggcgacg gcgcactgga
     5221 acaggccgat gttggtggcc atggccttga ctgcggcaaa ggccccgtag cctcgcccgt
     5281 aaacacagat ctggccttcg ttggcgatgc cgttgtcgat ggcccagcgg gtggcatcgg
     5341 cgatgtcacg ctgaacggcc tgcccgtttc gctctgcgcc cgaggccgtg taccactgac
     5401 ccagaccgcc cgatccccgg tagttgatct gcaggacggc gaaaccgcgc cgcgccagca
     5461 tctggacctc ggcgttgagc tcccagaccg cccgggtggt ggtgggcttg tcatggatcc
     5521 acacgatcat cgggtgaggc ccggccgatg ccggagtggt gacgtagccg cgcagagtga
     5581 acatgtcccg gctggtcagc tccatgggct cgaccttggc aaggtcttcc gccggcagcc
     5641 agggtcggct cgtggcaagc agcgtgctct ctccggtccc aaggctcacg ctgtagaagt
     5701 cgcctgaatt acggtcgctg tagattcgga ccacggcctc ggaaccttct gcggtgaagc
     5761 tggtgacgtc gacgtcgctg tccgggaacg ccgcgcgcag ggcggtgtga atcctggtga
     5821 agggatgttc cggatccagg tagtgccagc ttgcgtagcc cgggtcggaa cgcacggcca
     5881 ggagcctgcg gccaccgtag ctcatgatga cccggtcgat gtcggagccg gctccgcgat
     5941 agatctcgtc ggtctccccg gaatcgagat tccatctgta gagcccggcg gtgccgacct
     6001 tgttggccag ggcgaacacg ctgccggccc gatcgacggc gagcggcacc gtcccgagat
     6061 cggcggcggg atcgaatgat gacacgggcg tccagctgcc tgcggccagc cgatagacct
     6121 cgacccgccc atccgccgcg cggccggcag ccagcatcct gttgccgctc gggtcgctga
     6181 cgtagtaggc gggcatggca agggcagggg cgacaaacgt cttttcgccc gtttcgagat
     6241 tgagtttgac cggcctgcga agagctttct ccgcctcgga gcaactctcc gcgtcgcagg
     6301 cgctgtgggc cgcgatgagc acgttggcta ggtcgttggg cagcacgctg atcaccgcgg
     6361 tgggaagcct gacggcctcc gggttacggg gaagcaggcg cttgcgcgag ccgtccacgc
     6421 ccagcgcgag catggcgccc gtgggaaccg gttgctgagc cgcgccggca tctgctgcag
     6481 gctcgatgag cacgaggcgg ttgttcgccc aggtgatggt ctcgaagcgt tgaccaggac
     6541 tcgatgcgac gccggtggcc acggtcatgg ccggacgggc gaatatcacc agagtgtcct
     6601 tctgatcgtc gcttcggacc atcgccaggt gccgaccgtc cggtgagatg atcaccgagc
     6661 gcacgtcggc cggcctcaac agctcgccga tgtcgtcccc gtgggcgatc gggacgacca
     6721 ggaccgtgac gacgagtagc gcaacctcgc gcagtcgatg gagacggcta atcatcgcca
     6781 cctccgtcgt cgtcgaagct cgggaatgca tcgaaggccg gttggcccgg gtcgccatcc
     6841 tcttgcgcgt cgaaatcgga gaactcgggt acggaaggct gggcatcgag cgcgccctgc
     6901 agggcgccgg ctgccggtct tgcggacggt atggttgcga tcagcggctg ctcggcaccg
     6961 ccctgaattt tgcgggctat gtcggcgacg atccttacgg cgatctcgct ctgggccgcg
     7021 gcgtcctgag tgatctgctc atcgtagatc tccgaccaga tcatataatc cgtgtcgttg
     7081 gcgatgagct cggcggtcac ccggatgcgg ctgcgcaatc gcttgaccgt gccggttatc
     7141 cgatagcggg cccgttcccg caccgatgtc tcgcgcgcac cctggtagga tgcagagccc
     7201 actacctgca ggcctctgcc tggaagcgcg acccggacct ccgactcgat ggtatctccg
     7261 atccactgat cgtgagcggc ggtgaggtcc ttgaaggcct ccaccgtgat ggtgttctcc
     7321 aggggctcgt cgcgaagaat cgtctcaatg cgcgggccac gcaggaccaa caacccaagc
     7381 gcgccgatca gtgcggcgat gcccaacgcg ctcaacgagc ggcgcagcgc cctccccggg
     7441 actcctgccg atggcggcgc cggggatcgt ggtgcggtag gtcgtgcggt gcttgcgccg
     7501 cgtgtctcgg ctgcgccgtg cagggtggcg atcagcttgc gggcgtactc cgactcccgc
     7561 atggcgtatt tgaacaacgc ctggattctg ccaatggaga gctgcaggcc ctgaggaagc
     7621 tccgtgggtt ccaggtagac gaccacgatc gacttatggc gctcctgggc atacgaaagc
     7681 tcatccagac agtgcttgga tgaaccggag tctgttgaga cgaagaacag caggtgcgtg
     7741 cagttggcga tggcgttggc cacctcctcg gtccactccg cccccgggga gatgccctcg
     7801 tcgtaccaga cgttgatgcc ggccgaccga aggcgcacga gttcctgata cacctgctcg
     7861 cgattctggt gcgagtagca gacgaaggtg tagggctcgt caccgttgta cgcagggtag
     7921 ggcggagtga cggcctccag ggcctcggcg agctcggcgt catgggggcc cgcttcgatc
     7981 ctgcgaactg cgccgatggc ccggtagccg tccgtggaga cggtttcgat gtacctcggc
     8041 tcgcgtctgc tttcaccgag ggcacggcga atgcggctga tgttgctgtg gacggcgctc
     8101 ggttcggcgg cgctgtcgcg ccagaccgaa tccagcaact cgtcgaccga cacgatctcg
     8161 cccggacgtt ccagcagata gcagagcacc tcgagcgtgc tcggctcgag ttggatctcc
     8221 tgctcccccc gtcgaattcg gttgagatcc ggctcgacca gccagtcgcc gaactcgtag
     8281 gcgaagccct gatcagcgtc ggccatagac ccgcacgcat cctgccgccc aacgtcgtcg
     8341 ggcctagctt act
//
LOCUS       DBNHQR010000076         2100 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1337514, whole genome shotgun sequence.
ACCESSION   DBNHQR010000076 DBNHQR010000000
VERSION     DBNHQR010000076.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1337514"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="AC16VR_01660"
     CDS             complement(<1..454)
                     /locus_tag="AC16VR_01660"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR6009886.1"
                     /translation="MLQLIDISLKRGSNQLFEHLTCTIHPGHKVGMVGRNGAGKSTLF
                     DMILGRLQPDEGEVSRPSGWVASHMAQEVNVTDRPAIEHVIDGHRELRAVERRIAQAE
                     SRRDDLTLAELHTRFGDVGGHQARARAGTILHGLGFAADEIEQPFRAFS"
     gene            complement(556..1308)
                     /locus_tag="AC16VR_01665"
     CDS             complement(556..1308)
                     /locus_tag="AC16VR_01665"
                     /inference="COORDINATES: protein motif:HMM:NF013793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HIR6009887.1"
                     /translation="MNANVRRILISNGKGGCGKTTIAANLAVSYANRGAKVALVDQDP
                     QASSSYWADQRSRELAPVHLVRAHQRANMYQTQTFHNRIPADISRVVVDGSTNPQDRE
                     LEMLLKQCDVILVPLLPSAIDIRAGSQFIKELITHRSFRTLPKPVGVIANRVQPNSTM
                     HARLEHFLKCLDVPWVATFRDSPLYPEAFERGEGVVDLIDCRAARREAPAWRALTAWI
                     ERQPVSRGCTARELRNRPIRAPRHVDDETLSA"
     gene            complement(1568..>2100)
                     /locus_tag="AC16VR_01670"
     CDS             complement(1568..>2100)
                     /locus_tag="AC16VR_01670"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6009888.1"
                     /translation="HKDTFGDQFPNQALYYAWGPHQRVSSPDPTLAGYEQLPESEWNE
                     AHMLAGVWTIFPHVSVAGFDGGGRSVMVSQLFPGKTPQESVTVQNYLMEKAPDAEQAE
                     AAEQMFKLLEYVVQEEDYATGLRQQKAIMTGAMSHVMFGRNEEGGQRFHRWLDTLLAT
                     DDEDLNALFAGGERYC"
BASE COUNT          312 a          678 c          722 g          388 t
ORIGIN      
        1 cggagaaagc tcgaaacggc tgctcgatct catcggcggc aaagccgagc ccgtgcagga
       61 tagtcccggc gcgggcccgc gcctggtgcc cgccgacgtc gccgaagcgc gtatgcagct
      121 ccgccagcgt caggtcatcg cgccgggact cggcctgtgc gatgcggcgc tcaacggctc
      181 ggagctcccg gtggccgtcg atcacgtgct cgatggccgg ccgatcggtg acgttgactt
      241 cctgggccat gtgggaggct acccagccag aggggcggct cacctctccc tcgtcgggct
      301 gcaggcggcc gaggatcatg tcgaacagag ttgatttgcc cgcgccgttg cgcccgacca
      361 tgccgacctt gtggcctggg tggatcgtgc acgtcaggtg ctcgaacagc tggtttgagc
      421 cgcgcttcaa ggaaatgtcg atcagctgga gcatcggtgg ggcggctctg tgaaaatgac
      481 gcgcagaacg tgatcagatg aggtggcgcc gggcctgatt tccggaagac ccgagaagct
      541 ggctggcgcc ggattctatg cgctcaaggt ctcgtcgtcg acgtggcgcg gcgcccggat
      601 cggtcggttg cgcagctccc gggccgtgca acctcggcta acgggctgcc gctcgatcca
      661 ggccgtgagc gctcgccacg cgggcgcttc ccggcgcgcg gcccggcagt cgatcaggtc
      721 cacgacgccc tctccccgct cgaaggcttc cggatagagg gggctgtcac ggaaggtggc
      781 gacccacgga acgtccaggc acttgaggaa gtgctccagc cgcgcgtgca tggtggagtt
      841 cggctgaacc cggttggcga tgacgcccac gggcttgggc agcgtccgga agctccggtg
      901 ggtgatcagc tccttgatga actggctgcc ggctcgaatg tcgatggccg acggcagcag
      961 cggaaccaga atcacgtcgc actgcttcag gagcatctcc agttctcggt cctgtgggtt
     1021 ggtgctgccg tcgacgacga cccggctgat gtcggcgggt atccggttgt gaaaggtctg
     1081 agtctgatac atgttggcgc gctgatgcgc gcggacgagg tgaacgggtg caagctcccg
     1141 ggagcgctgg tccgcccaat agctgctgga cgcctgcgga tcctggtcga ccagtgcgac
     1201 cttggcgccg cgattggcgt atgacacggc aagattggcg gcgatggtgg tcttgccgca
     1261 cccgcctttg ccgttgctga tcagtatgcg ccgaacgttc gcattcatgc gctggggccc
     1321 tttctttgag agctctctgc gaatcgggat ccctgtgtcc ctgggcctcg tttcaggccc
     1381 cccctgctcc atgcgtcccc caggacgccg gcgtcgaccg ttgtctggcg gccgggccgg
     1441 acgggcagcg ctggaatata acaccgtttg cgcggatgtc accaatggtg cagggcccct
     1501 ggcgccgcat gacgcgcctc tcgccggggc ccttcggcgc ccgggaggcc gtccgggaag
     1561 cccgcggtca gcagtaacgc tcgccgcccg caaacagggc gttgaggtcc tcgtcatcgg
     1621 ttgcgagcag ggtgtccagc cagcggtgga agcgctgccc gccctcttcg ttccggccga
     1681 acatcacgtg gctcatggcg ccggtcatga ttgctttctg ctgccgcagg ccggtggcgt
     1741 agtcctcctc ctgcaccacg tactcgagca gcttgaacat ctgctcggcc gcctcagcct
     1801 gctcggcgtc gggcgccttt tccatcaggt agttctgcac ggtcaccgat tcctggggcg
     1861 tcttgcccgg gaacagctgg gacaccatga cgctgcggcc gccgccgtcg aagccggcga
     1921 cggagacgtg cggaaagatg gtccacacgc ccgccagcat gtgtgcttcg ttccactcgg
     1981 actccggcag ttgctcgtag cctgcaagcg tcgggtccgg cgagctgacc cgctgatggg
     2041 gcccccaggc gtagtacagc gcctggttgg gaaactgatc accgaacgtg tccttgtgca
//
LOCUS       DBNHQR010000077         4414 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_703916, whole genome shotgun sequence.
ACCESSION   DBNHQR010000077 DBNHQR010000000
VERSION     DBNHQR010000077.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4414)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4414)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4414
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_703916"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="AC16VR_01675"
     CDS             complement(<1..627)
                     /locus_tag="AC16VR_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003446576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR6009889.1"
                     /translation="MDSAENDKVTPLPASAAVVTGEAGRRQILKSHKLDDVCYDIRGP
                     VLEEAHRLEEEGHRVLKLNIGNPAPFGFEAPEEILLDVIHNLPESQGYSDSKGIYSAR
                     KAIMQYCQQQGVPNVEIDDIFLGNGVSELVVMSLQGLLNNGDEVLIPAPDYPLWTAAT
                     HLSGGRAVHYRCDEQSDWNPDLADIKARITDRTKAIVIVNPNNPTGAVY"
     gene            738..1145
                     /gene="msrB"
                     /locus_tag="AC16VR_01680"
     CDS             738..1145
                     /gene="msrB"
                     /locus_tag="AC16VR_01680"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009623407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HIR6009890.1"
                     /translation="MGKVTKSDDEWRSELDTHTYEIARKAGTERAFTGKYWDTKTPGI
                     YHCTCCGASLFDSDTKYDSGSGWPSFYEPVSHDAVSTREDNSLMMQRIEVLCAECDAH
                     LGHLFPDGPEPTGLRFCMNSASLDLQERDEDGS"
     gene            complement(1151..2662)
                     /locus_tag="AC16VR_01685"
     CDS             complement(1151..2662)
                     /locus_tag="AC16VR_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HIR6009891.1"
                     /translation="MASSQPLAVQAGLDAMRSGGNAVDAALATAIALTVVEPNNNGVG
                     SDAFAIVWDGSKLVGLNASGRSPAGWTPDRFAGQRTMPELGWDAVTVPGAVSAWSALS
                     ERYGRLAFPDLFDAAIRYAAEGFPVGPKTAYYWQFAERRYADFPDFANHFLPAPRAGE
                     RFRRPDLARTLQEIAATGGKAFYEGDIAERIAAAAATDGGVMTRDDLAAHRAEWVDPL
                     SQPYRSVDVHEIPPNGQGLAAQIALGIFAHFDAPPLETAESVHLQVEAMKIAIRAAAD
                     HFADADAMRLPPAELLDPANLRRAAGTIGRSASTLPPVSLPTSQDTVYLCAADASGMM
                     VSFIQSNFMGFGSGIVIPGTGISMQNRGSGFTLEPGHPNRVGPRKRPYHTIIPGFVTE
                     NGRPRLAFGVMGGHMQHQGHFQMVQRIFDHGQYPQAASDAPRWRVFPDYSLRVEPEFP
                     EKVIKELADRGHQIGVEDQEHQFGGAQLIYRNESEEAYIAGSDHRKEGMAAGF"
     gene            2805..4334
                     /locus_tag="AC16VR_01690"
     CDS             2805..4334
                     /locus_tag="AC16VR_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6009892.1"
                     /translation="MHPGIIAAANPDKVAYLMADSGETITYGELEKTSNQGAHLFRQL
                     GLKRGDHIALLLENHPRFFQICWAAQRAGLYYTAISWRLQQAEVEYIVNNCEAAVFIT
                     SAARREVVAPLADSMPGVSHRFVIDGAIEGFDDWAEAISAMPVTPVADESEGMAMLYS
                     SGTTGYPKGVKRPLPDGEYGAAAGINLLNILYGADDDSIYLSPAPLYHAAPLGFTMGC
                     LRYGIQVVVMEHFEAELALRYIQDYRITHSQWVPTMFVRMLKLPEEIRLRFDTSTLRC
                     AIHAAAPCPVPVKEQMIEWWGPVIYEYYAGTEGNGFVQLNATEWLEHKGSVGRPLNCE
                     IHICGDDGEELPAGESGTIYFGGGGEFEYHGDPQKTAGSRHPKGWSTLGDVGYLDEDG
                     YLYLTDRKHFMIISGGVNIYPQEAENVLITHDAVMDVAVFGVPNPEFGEEVKAVVQPT
                     DMALATPELAVELMDYCRARLSHIKCPRSVDFREELPRHPTGKLYKRLLKDEYWSKAG
                     G"
BASE COUNT          775 a         1345 c         1479 g          815 t
ORIGIN      
        1 gtaaacggct ccggtagggt tgttcggatt cacgatcacg atggcctttg tccggtcggt
       61 gatgcgtgcc ttgatgtctg ccaggtccgg gttccaatcg gactgctcgt cgcaacgata
      121 gtgcacggcc cgcccgccgc tgaggtgtgt tgctgcggtc cacaacgggt agtcgggcgc
      181 ggggatgagc acctcgtcgc cgttgttgag caggccctgc agcgacatga ccacgagctc
      241 ggacaccccg ttgccgagaa agatgtcgtc gatctccacg ttgggcacgc cctgctgctg
      301 gcagtactgc atgatggcct tgcgcgccga gtagatgcct ttcgagtccg aatagccctg
      361 ggattcagga aggttgtgaa tgacgtcgag aagaatttcc tcgggcgcct cgaagccgaa
      421 cggtgccggg ttgccgatat tgagcttgag gacccgatgg ccttcctcct ccagcctgtg
      481 cgcttcctcc agcacgggcc cgcgaatgtc gtagcagacg tcgtcgagct tgtgcgactt
      541 gagaatctgc cggcgccccg cttcacccgt gacgaccgcg gccgacgccg gtagcggcgt
      601 gactttgtcg ttctctgcgc tatccatcgt tggggatcca ggtttccatc ggctgccttc
      661 caggcataga atttcgcgcg ctgcgatgtt ggcggcggcg attgtaaact ggtggcatgg
      721 tagcagggga accagatatg ggtaaggtga ccaaatcaga cgacgagtgg cgctcggagc
      781 tggacacgca cacgtacgag atcgcccgga aggccggcac cgaacgggcg tttaccggaa
      841 agtactggga taccaagacg ccgggtatct atcattgcac ctgctgcggc gcctctctgt
      901 tcgattccga caccaagtac gactctggct cgggttggcc cagcttttac gaacccgtct
      961 cccacgatgc ggtgagcacg cgcgaggaca acagcctgat gatgcagcgc atcgaggtgc
     1021 tctgtgccga atgcgacgcc cacctgggtc acctatttcc ggacggtccc gagccgaccg
     1081 gattgcgctt ctgcatgaac tccgcctcgc tcgatcttca ggaacgcgac gaggacgggt
     1141 cgtgacctcg tcagaagccg gcggccatgc cttccttgcg atggtctgac cccgcaatgt
     1201 aggcttcttc cgactcgttt cggtagatga gctgagcacc gccgaactgg tgttcttgat
     1261 cttcgacacc gatctgatgg ccgcggtcgg ccagctcttt gatgactttc tccgggaatt
     1321 ccggctccac ccgcaggctg tagtcgggaa agacccgcca gcggggagcg tcgctggcgg
     1381 cctgggggta ttgaccgtga tcgaatattc gctgcaccat ctggaagtgt ccctgatgct
     1441 gcatgtggcc gcccatgacg ccgaacgcca gccgggggcg cccgttttcg gtcacgaatc
     1501 ccgggatgat ggtgtggtac gggcgttttc gggggcctac acggttcggg tgcccgggtt
     1561 ccagggtgaa ccctgatccg cggttctgca tggaaatgcc ggtgccggga atcacgatgc
     1621 cggatccgaa ccccatgaag ttggattgga tgaacgagac catcatgccg ctcgcatcgg
     1681 cggcgcagag gtagacggta tcctggctgg ttgggagcga cacgggcggg agcgtcgacg
     1741 cgctcctgcc gatggtcccg gcggcgcggc gcagatttgc cgggtccagc agttctgccg
     1801 gcggcaggcg catggcgtcg gcgtcggcaa agtgatcggc cgccgcccgg atcgcaatct
     1861 tcatggcttc gacctggagg tggacgcttt cggccgtttc gagcgggggc gcgtcgaaat
     1921 gggcaaagat gcccagggcg atctgagcgg caaggccctg gccgttgggt ggaatctcgt
     1981 ggacgtcgac gctgcggtaa ggctgggaga gcgggtccac ccactcggcg cgatgcgcag
     2041 ccaggtcatc ccgggtcatg accccaccgt cggtggcggc cgcggccgcg attcgctcag
     2101 cgatatcgcc ttcgtagaag gctttcccgc ccgtggctgc gatttcctga agcgttctgg
     2161 cgagatccgg gcgccgaaac cgctcaccgg cgcgaggcgc gggcaggaag tgattggcga
     2221 aatccggaaa gtccgcgtag cggcgctcgg caaactgcca gtagtaggcc gttttggggc
     2281 caacgggaaa cccttccgcg gcatagcgga tggcggcgtc gaacagatcg ggaaacgcga
     2341 gccggccgta gcgctccgac agggccgacc aggcgctgac cgcgcccggg accgtcacgg
     2401 cgtcccatcc gagctcgggc attgtgcgct ggccggcgaa gcggtcgggc gtccagccgg
     2461 cgggagagcg gcccgaagcg ttcaggccga cgagttttga accgtcccac acgatggcga
     2521 acgcgtcgct gcctacgccg ttgttgttgg gttccaccac ggtcagcgcg atggcggttg
     2581 ccagggccgc gtccaccgcg ttgccgccgc tgcgcatggc gtcgaggccg gcctgcacgg
     2641 ccagcggttg agagctggcc acgatgttct cggcgcaaac gggcgagcga gcggcccgat
     2701 acggctgttc ccaatccatc agcagacgtt actgcgttgc gtgaatcggg aaaagcccct
     2761 aactttggct gcctgcatat cgggcgcaat ccaagggaga cagcgtgcat ccgggaatca
     2821 tcgcggcggc caacccagac aaggttgcct acctcatggc cgactcgggc gagaccatca
     2881 cttacggcga gctggagaag acttcgaatc agggcgcgca cctgtttcga cagctcgggc
     2941 tgaaaagggg cgatcacatc gcgttgctgc tggaaaacca cccgcggttc tttcagatct
     3001 gctgggcggc ccagcgtgcg ggcctgtact acacggccat aagctggcgc ctgcagcagg
     3061 cggaagtgga atacatcgtc aacaattgcg aggcggccgt gttcatcacc tcggctgcac
     3121 ggcgcgaggt ggttgcgccg ctggcggaca gtatgccggg tgtctcccat cggttcgtca
     3181 tcgacggagc catcgagggt ttcgacgact gggccgaggc gatctccgcc atgccggtca
     3241 cccccgttgc cgacgaaagc gaagggatgg cgatgctcta ctcctcgggg acgacgggtt
     3301 acccgaaagg ggtaaaaaga ccccttccgg acggagagta tggggcggcg gctggtatca
     3361 acctgctgaa cattctctac ggcgcggacg atgattccat atatctttcg ccggcacccc
     3421 tgtatcacgc ggccccgctc ggcttcacca tgggatgcct gcgctacggc attcaggtcg
     3481 tcgtgatgga gcatttcgag gcggaacttg cgctccgtta catccaggac taccgaatca
     3541 cccacagcca gtgggtgccg accatgttcg tgcgcatgct caagctgccc gaggagatcc
     3601 ggctgcgctt cgacacgtcg acgctgcggt gcgcgatcca cgccgccgcg ccctgcccgg
     3661 tgccggtcaa ggagcagatg atcgagtggt ggggtccggt catctacgag tactacgccg
     3721 gcacggaggg taacggcttc gtgcagctga acgctaccga gtggctggag cacaagggat
     3781 ccgtcggacg tccgctcaac tgcgagatcc acatttgcgg cgacgacggc gaagagctgc
     3841 ccgccggcga gtcgggcacg atctacttcg gcggcggcgg cgagttcgag taccacggag
     3901 atccgcagaa gacggccggc tcccgtcatc ccaaggggtg gagcacgctg ggcgatgtcg
     3961 ggtatctcga tgaggacggc tacctttacc tcaccgatcg caaacatttc atgatcatct
     4021 ccggcggcgt gaacatctac ccccaggagg cggagaacgt gctcatcacc cacgatgcgg
     4081 tgatggacgt cgccgtgttc ggcgtgccga atccggagtt cggagaggag gtgaaggccg
     4141 ttgttcaacc taccgacatg gcgctggcga cccctgagct cgcggtggag ctgatggact
     4201 actgccgtgc ccggctatcc cacatcaagt gcccgcgctc ggtcgacttc cgggaggagt
     4261 tgcctcgaca ccccacggga aagctttaca agcggctgct caaggacgag tactggagca
     4321 aggcgggagg ctgagggcgc cgctcagcgg tcgcgcagca ggttggccgg cgcgtactgg
     4381 tccgtgagca cccttgccga cgtgtcccag tcgg
//
LOCUS       DBNHQR010000078         9109 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_957935, whole genome shotgun sequence.
ACCESSION   DBNHQR010000078 DBNHQR010000000
VERSION     DBNHQR010000078.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9109)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9109)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9109
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_957935"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..1059
                     /locus_tag="AC16VR_01695"
     CDS             160..1059
                     /locus_tag="AC16VR_01695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HIR6009893.1"
                     /translation="MKIVIAGGTGLIGRALSTRLAGAGHEVVVLSRQRQAPTGSTVAS
                     WDGRSPGAWQDVLRGAGALINLCGASIGEARWTPTRKRELADSRTLPSVALAQACAAV
                     EDPPKVLLQASGVGFYGTSETTTFDESSPPGEDFLARLAQEWESAGADLLPPETRRVV
                     MRMGVVLSREGGVLEQLLLPFRLFAGGPIGSGRQFLSWIHIDDLTAAMEFLIGTSQLS
                     GPVNLVAPEVPRNTQVARAIAEALHRPSWFPIPAWAMRLALGEMSSLVLEGQRVIPRE
                     LQAAGFRFSYPNLRSALAQILAN"
     gene            1115..1540
                     /locus_tag="AC16VR_01700"
     CDS             1115..1540
                     /locus_tag="AC16VR_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005344 - oxygen carrier activity
                     [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HIR6009894.1"
                     /translation="MPPSTPYQILGQEGINQLVDAFYDAMDELTEAAHVRAMHKENLE
                     EVRQKLKDYLIGWMGGPPIYQMKTGTVCLTDPHAPYAIGPKERDQWLLCMDTALERIG
                     ASDELKEMLKIPMFRVADTVRNRDSSEPKPRDPNIIAVG"
     gene            1575..3614
                     /locus_tag="AC16VR_01705"
     CDS             1575..3614
                     /locus_tag="AC16VR_01705"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HIR6009895.1"
                     /translation="MNDRSRPRSPIAGPALFVTVLAAASAVAEVEWRSYGNDHTEQRF
                     SPIDQVNRSNVGELGLDWALSVPDAVSLNSTPLMIDGVLYFSADRAIVHAVDARSGKL
                     IWSYDPQSWKHAPRGIGVSFNTNRGIAHSEGSIFVGTADGRLVSLDARTGRVKWATRT
                     FGREERKAITGAPRAFDGKVFIGNGGAEFGTRGFVSAYDAADGELLWRFYTVPGDPSL
                     GFENDAMAKAAKTWHGDWWKVFGGGTVWNGLTYDPELDLLYVGVGNGDPWDHDLRSQG
                     KGDNLYLCSIVALKADTGEYVWHYQVNPGEQWDYKATMDMILADLPMGGTTRKVLMQA
                     PSNGFFYVLDRATGELLGAEKYERATWAERIDLKTGRPVETPGIRMKPGEETLIYPGP
                     YGAHNWQAMSFNPGTGLAYIPTIRYPAIYSKNTAYEQREGFFVIGMNTVYATPEPGDG
                     KGGLVAWDPVKGEARWRIQYDSIWNGGTLTTSGGIVFQGTADGNFLAVDASSGEELWR
                     FDVQRGVTAAPISYTLDGRQHIVLPVGWGGQSAFGPPAFRAHRIPYKGEDVRLLSFSL
                     DGKASLPPPPARPTEIQTANTGDEPIDEALAVKGFNIYHQASCAVCHGGEAISVGAAA
                     PDLRESPYMANYPAFRAVVVEGALLPRSMPMFDDLTETEVRAVWEYVRQQTRLVD"
     gene            complement(3620..3958)
                     /locus_tag="AC16VR_01710"
     CDS             complement(3620..3958)
                     /locus_tag="AC16VR_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009896.1"
                     /translation="MDEIRSYHYDPEKFEPYRKWALEEAIPFLKDNLNIVGFWLDNGK
                     APEVGGTSPMDMPLGSANVTWVIRWDSEEDREAGHARIFGGQEWQDIWSRHPDANGYL
                     QMEARFADPV"
     gene            4090..4824
                     /locus_tag="AC16VR_01715"
     CDS             4090..4824
                     /locus_tag="AC16VR_01715"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HIR6009897.1"
                     /translation="MQMWRFLAPSALGFTSRNMWHALIDRATGAPPRALRAMEHVAAH
                     AERHNPQDVLRKLDEFATSVRWMMSIGPEKDKVIAEARAALDRDPRVLELGAYAGYSS
                     IYMADVLGPGTNITSVEVNADNVRAARGNIAHAGLDGRVEVVHGSSSDVIPTLEGSFD
                     LVFLDHWKDLYLQDLQLIETQGLIAPGSVVVADNVGELFGADAYLDYVRHCGRYDSAN
                     RPATIEYTSLPDAVEISVYRGQGDDG"
     gene            4814..5920
                     /locus_tag="AC16VR_01720"
     CDS             4814..5920
                     /locus_tag="AC16VR_01720"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024809.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6009898.1"
                     /translation="MTDEPIAPVTVYGSSISYFTGKLENYLRIKGIPYELKPLGPGSV
                     RKLIKSRTGSSQMPAVRLGDGRWLTDTTPVIGWFEREYPEPAVIPHDPVQRFFSLLLE
                     DYADEWLWRPAMHYRWFYDVGARFASAHLAQELTRGVYLPMFVKRRAITRRQRNGYTR
                     GDGITADQVPGVEAIYSRTLGQLETIFRSRPFLLGASPSLADVGFSGPFFRHFGLDPV
                     PAEIMRRDAPAVYEWIARLWNAGPVDSSMAIPAGIPDDWAPILDEIGTHYLPYLCANV
                     DAVAAGLRRFDAEVGGVRYHRARWSPYRVWCLKKLRETFAGLTEDEQAEARTLLERHG
                     CWEPMWRHALLPLKDGFCERLPFHADAKMIDVYQ"
     gene            complement(5944..6462)
                     /locus_tag="AC16VR_01725"
     CDS             complement(5944..6462)
                     /locus_tag="AC16VR_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6009899.1"
                     /translation="MHGIQVEGLMMPSGFRSHRAQVAVCTDLADLRAFISDTSRFHEW
                     IPDLAEVRLLEQSDDGVVYYMRSKAPWPVKDRDMVYRLFGVPESGDAASVALAIEGLP
                     GRLPEVDGAYRMHDVSGQWRIWERGDRLDVQQQIYLNPGDVPRMFANRSMSASLARTL
                     GNLASRYPCPKS"
     gene            complement(6652..6948)
                     /locus_tag="AC16VR_01730"
     CDS             complement(6652..6948)
                     /locus_tag="AC16VR_01730"
                     /inference="COORDINATES: protein motif:HMM:NF045268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase accessory protein"
                     /protein_id="HIR6009900.1"
                     /translation="MTGEREAAPLIAEMPAPAALPRKSGEMVFHDEWERRAFAMAVSL
                     AEQGAFEWSEFQQKLIAAISDAERDDPQQPSRGYYESWLVALEALLQEKELLPG"
     gene            complement(6945..7610)
                     /gene="nthA"
                     /locus_tag="AC16VR_01735"
     CDS             complement(6945..7610)
                     /gene="nthA"
                     /locus_tag="AC16VR_01735"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008006381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HIR6009901.1"
                     /translation="MSNDHDNDHDHDHDHGGPPNPYHAASRSDDENRALEERVGRIES
                     LLLEKGLVRQDALDRIADIYENDLGPMNGAKVVARAWVDAAFKQRLLEDASAAIGELG
                     YGGLQGEHMVVVENTPEVHNVVVCTLCSCYPWPVLGLPPAWYKSPAYRSRVVIDPRSV
                     LEEFGTAVDDDVEVRVWDSSAEIRYLVLPERPTGTETLTEDQLAELVERDAMIGVTRV
                     AAP"
     gene            complement(7600..8283)
                     /gene="nthB"
                     /locus_tag="AC16VR_01740"
     CDS             complement(7600..8283)
                     /gene="nthB"
                     /locus_tag="AC16VR_01740"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003410914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit beta"
                     /protein_id="HIR6009902.1"
                     /translation="MNGIHDLGGMTCFGPIVREDDEPVFHSDWERRVFAMNMLSMGQF
                     GSLDQARHAIERMDPAAYLSTSYYEHWLAGIEILGRDSGLLMDAEIASGRSEHTSTPS
                     RPPLDPEFVDAMVRSGSPSTRPVSRRPTFDVGDSVRARNLDVRGHTRLPRYVRGKRGV
                     VSAVHGTHVFPDTSAHGLGENPQPLYNVRFEADELWGDNLARRDCVHIDLWEDYLEAS
                     PVEGRSHEQ"
     gene            complement(8337..9047)
                     /locus_tag="AC16VR_01745"
     CDS             complement(8337..9047)
                     /locus_tag="AC16VR_01745"
                     /inference="COORDINATES: protein motif:HMM:NF016300.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HIR6009903.1"
                     /translation="MTDSRGNFIPGLLVAIGLAVAGYLIGQGFVHARVLDRSVEVKGL
                     AEREVPADTAIWPVQIVAAGDDLQALLVQIEGQSGRVVGFLADLGFTPGEVSVGAPNV
                     LDRQAQNFGDPNTRFRYSVAQTITVFTEAVATVREAGGRLLELGKEGIVVTQNNYDLR
                     TQYLFNGLNDIKPEMIEEATRNARRVAEKFAGDSDSVLGKIRSARQGQFSIQDRDSNN
                     PHIKRVRVVSTLEYYLVD"
BASE COUNT         1640 a         3030 c         2899 g         1540 t
ORIGIN      
        1 gctcacgacc ggcacggtca ccgctacgac cgcgaaccag gccgccacgg cgagcgacgt
       61 cgaggtcacg gccactgata gctccgcgcc gattcacttc ggcgggcaca accgctaagg
      121 tcggcaactc attgaacgga acgcgttggt aaccggctgg tgaagatcgt aatcgccggc
      181 ggcaccggcc tcatcggccg ggcactgtct acccgactgg ccggcgccgg tcacgaggta
      241 gtggtgctgt cgcgacagcg ccaggcgccg acgggcagca ccgtggcgtc atgggacggc
      301 cgttcccccg gggcgtggca agacgtgctg cgcggcgccg gcgccctgat caacctgtgc
      361 ggcgcgagca tcggggaggc ccgctggacg ccgactcgca agcgggagct ggctgactcg
      421 cgcaccttgc cgtcagttgc tctcgcccag gcatgcgccg ccgtcgagga tcctcccaag
      481 gtgctgctgc aggcatcggg cgtcggtttc tatgggacct ccgaaaccac gaccttcgac
      541 gaatcgagcc cgcccggcga ggactttctc gctcggctgg cacaggaatg ggagagcgcg
      601 ggggccgatt tgctgccccc tgagacgcgg cgcgtcgtca tgcgcatggg cgtcgtgttg
      661 tcccgtgaag gaggcgtcct ggagcaactg ctcctgccgt ttcggctctt cgcgggcgga
      721 cccataggct ccggtcgaca attcttgtcc tggatccaca tcgacgatct gaccgcggcc
      781 atggagtttc tcatcggcac cagccaacta tccggccccg tcaacctggt cgcgcccgaa
      841 gtaccgcgga acactcaggt cgcccgtgcc atcgcggaag ctctgcaccg gccgagctgg
      901 tttcccatac cggcgtgggc gatgcgcctg gccctgggcg agatgtccag tctcgtcctg
      961 gagggtcagc gggtaatccc ccgcgagctg caggcagccg ggtttcgctt ttcctacccc
     1021 aacctgcgat cggcgctggc acagattctg gccaattgat tacccgtaat ccccttgggt
     1081 atgctgattc gtcagcaacc gacgcgagac cgccatgccg ccatcgacgc cttatcagat
     1141 tctcggccag gaaggtatca accagctcgt ggacgctttc tacgacgcca tggacgagct
     1201 caccgaggcg gctcacgtac gcgccatgca caaggaaaac ctcgaagagg tccgccagaa
     1261 gctgaaggat tacctcatcg gctggatggg cgggccgccg atctaccaga tgaagaccgg
     1321 caccgtctgc ctgacagacc ctcacgcccc ctacgccatc gggcccaagg agcgggatca
     1381 gtggctgctg tgcatggata cggccctcga gcgtatcggc gcatcggacg agctgaagga
     1441 aatgctcaag atcccgatgt tccgggtcgc agacaccgtg cgcaaccggg attcgtcgga
     1501 gccgaaaccg cgcgatccga acatcatcgc cgtgggctga acggatcctg gaaacgaaac
     1561 cccatcgcag gtccatgaac gaccgatcga gaccgcgctc gcccattgcc gggcccgctc
     1621 tgttcgtcac tgtgctggca gccgcttcgg ctgtggcgga agtcgagtgg aggagctacg
     1681 gcaacgacca caccgaacaa cgcttcagcc ccatcgacca ggtcaaccgg agcaacgtgg
     1741 gggaactcgg actcgactgg gcgctgagcg tgccggacgc cgtgtcgctc aacagcacgc
     1801 ccctaatgat cgacggtgtg ctctactttt ctgccgaccg agccatcgtg cacgccgtgg
     1861 acgcaaggag cggcaagctc atctggtcct acgaccccca gagctggaag cacgcgcccc
     1921 ggggaatcgg ggtgagcttc aacaccaacc gcggcatcgc ccactccgag ggcagcatct
     1981 tcgtcggcac cgccgatggc aggctcgtct cgctggatgc caggaccggc agggtgaaat
     2041 gggccacgcg aacgttcggg cgcgaggagc gcaaagcgat aaccggcgcg ccgcgtgcct
     2101 tcgacgggaa agtcttcatc ggcaacggcg gagcagaatt cggaacccga ggattcgtct
     2161 ccgcttacga cgcggcagat ggcgagctgc tctggcgttt ttacacggtg ccgggagacc
     2221 cgtccctggg ctttgaaaac gacgccatgg cgaaagccgc caagacgtgg cacggcgact
     2281 ggtggaaggt gttcggcggc gggaccgtat ggaacgggct gacctacgat cccgagctgg
     2341 atctgttgta cgtgggcgtg ggcaacggcg atccgtggga tcacgatctc aggagccagg
     2401 gcaaaggcga caatctgtac ctctgctcca tcgtcgcgct gaaggcagac accggagagt
     2461 acgtctggca ctaccaggtc aatcccggcg agcagtggga ctacaaagcc accatggaca
     2521 tgatcctggc ggacctaccc atgggcggca ccacccggaa ggtgctgatg caggcgccgt
     2581 caaacgggtt tttctacgtg ctcgaccggg ccaccggaga gctgctgggc gcggagaaat
     2641 acgaacgtgc cacctgggcg gagcgcatcg acctcaaaac cgggcgcccc gtggagacgc
     2701 ccggcatccg gatgaaaccc ggcgaggaga ccttgatcta cccgggcccc tacggcgcgc
     2761 acaactggca ggccatgtct ttcaatcccg gcacggggct ggcctacatt cccaccatcc
     2821 gctatccggc catctactcg aaaaacacgg cctacgagca gcgcgagggt tttttcgtga
     2881 tcgggatgaa cacagtctac gcgacgcccg agcccggcga cggcaaaggc gggctggtag
     2941 cctgggatcc cgtcaagggc gaggcacgct ggcgcattca gtacgattcg atctggaacg
     3001 gcggcacgct gaccacgtcc ggcggcatcg tgttccaggg gaccgcagac ggaaacttcc
     3061 tcgcggtgga cgcttccagc ggtgaggagc tgtggcgatt cgacgtgcag cgcggcgtca
     3121 ccgcggcgcc catcagctac acgctggacg gcaggcagca catcgtgctg ccggtgggct
     3181 ggggcgggca gtcggcgttc gggccgccgg cgttccgggc acaccgtatc ccctacaagg
     3241 gcgaggacgt gcgcctgctg agcttcagcc tcgacggcaa agccagcctg ccgcctcctc
     3301 cagcccggcc caccgagatt caaacggcga acacgggcga cgagcccatt gacgaggcgc
     3361 tggcagtaaa gggattcaac atctatcacc aggccagctg cgcggtttgt catggcggcg
     3421 aggccatatc cgtcggtgcc gccgcccccg atctgcggga gtcgccgtat atggcgaatt
     3481 accccgcctt tcgcgccgtg gtggtcgaag gcgcgctgct gccgcgcagc atgcccatgt
     3541 tcgacgacct caccgaaacc gaggttcgag cggtgtggga gtacgtccgg cagcagaccc
     3601 gcctggtgga ctgaccccct cagacgggat cggcgaaacg cgcctccatc tgcaggtagc
     3661 cgttggcgtc ggggtgacgg gaccagatgt cctgccattc ctggccgccg aatatcctgg
     3721 catgacccgc ttcgcggtcc tcttcgctgt cccagcggat gacccaggtg acgttcgcgc
     3781 tgcccagcgg catgtccatg ggcgacgtgc cgccgacctc cggcgctttg ccgttgtcga
     3841 gccagaagcc gacgatgttc agattgtctt tcagaaacgg tatggcttcc tcgagcgccc
     3901 acttcctgta gggctcgaac ttctcagggt cgtagtgata gctgcggatc tcgtccatgg
     3961 cgggtcaccg ggtcggttgg caattaaccc gcaaccttag ccgctaacgg ctgatggcaa
     4021 aagcagtcgg gcgatttgcg gtacgatctc gtgccagcga aatcggcatc aacccaagga
     4081 ggtcccatca tgcagatgtg gcgctttctc gcaccctcgg cgctgggatt caccagccgg
     4141 aacatgtggc acgccctgat cgaccgcgcc accggcgcgc cgcccagggc gcttcgagcc
     4201 atggagcacg tcgcagcgca tgccgaacgg cacaaccccc aggacgtgct ccgcaagctc
     4261 gacgagttcg caacgtcggt gcgctggatg atgagcatcg gcccggagaa ggacaaggtc
     4321 attgccgagg ccagagcggc gctggaccgc gatccgcgcg tcctggagct gggcgcctac
     4381 gccggttact cgtcgatcta catggcagac gttctcgggc cgggcacgaa catcacctcc
     4441 gtagaggtga atgccgacaa cgtgcgggcc gcacggggca acatagcgca cgcgggtctc
     4501 gatgggcgcg tagaggtggt gcacggcagc tcgagcgacg tgatcccgac actcgagggg
     4561 agcttcgatc tcgtgtttct ggatcattgg aaggacctct acctccagga tctgcagctg
     4621 atcgaaaccc aagggttgat cgcgccgggc tccgtcgtgg tagcagacaa cgtgggcgag
     4681 ctgttcggcg cggacgctta cctggactac gtccgtcact gcgggcgcta cgattcggcc
     4741 aaccgcccgg ccaccatcga gtacacgtcg ttgccggatg ccgtagagat ctcggtatac
     4801 cgggggcaag gcgatgacgg atgagcccat cgcgccggtc accgtttacg ggtcgagcat
     4861 ctcgtacttc acggggaagc tggaaaacta ccttcgcatc aagggcatac cctacgagct
     4921 caaacccctg ggccccggct ctgttcgcaa gctcatcaaa agcagaaccg gatcctcgca
     4981 gatgcccgcg gtacggcttg gggacgggcg ctggctgacc gataccacgc ccgtcatcgg
     5041 gtggttcgag cgggagtatc ccgagccggc ggtgattccg cacgatccgg tgcagcggtt
     5101 cttcagcctg ctgctggaag attacgccga cgagtggctt tggcgaccgg ccatgcacta
     5161 ccgctggttc tacgacgttg gcgctcgctt cgccagcgcc catctggccc aggagctcac
     5221 gcggggtgta tacctgccca tgttcgtcaa gcgtcgcgcc atcacccggc gtcaacgcaa
     5281 cggctacacc aggggagacg gcatcaccgc cgaccaggtc ccgggtgtgg aggccatcta
     5341 ctcgcggacc ctgggacagc tcgaaacgat cttccgatcc cggcccttcc tgctgggtgc
     5401 cagccccagc ctggcggacg tcggcttctc cggccccttc ttccgtcact tcgggctcga
     5461 tccggtccct gccgagatca tgcgcagaga cgcgcccgcg gtctacgagt ggatcgcccg
     5521 gctgtggaac gccggccccg tggactccag catggcaatt ccggccggta ttcccgacga
     5581 ctgggcgccc atcctggacg agatcggcac gcactacctg ccctacctct gcgccaacgt
     5641 ggacgcggtt gcagcgggcc tgaggcgctt cgacgcggag gtcggcggcg tgcgctacca
     5701 ccgtgcgcgc tggtctccct atcgggtctg gtgcctgaag aagctgcgcg agacgttcgc
     5761 gggcctcacc gaggatgagc aggccgaagc cagaaccctg ctcgagcggc acggctgctg
     5821 ggagccgatg tggcggcacg ccttgctgcc gctgaaggat ggtttctgcg agcggctgcc
     5881 cttccacgcc gatgccaaga tgatcgacgt gtatcaatag ccgcgcgacg tcgctgctat
     5941 ccttcagctc ttcgggcagg ggtatcgcga ggccagattg ccgagcgtcc tggccaggct
     6001 ggccgacatg ctgcggttgg cgaacatccg cgggacgtcc ccggggttga ggtagatctg
     6061 ctgctgcacg tcgagccggt caccccgttc ccatatgcgc cattgaccgg aaacgtcgtg
     6121 cattcgatag gcgccatcca cctcgggcag ccgcccgggc aggccctcga tggcgagcgc
     6181 cacgctggca gcgtccccgg actcgggcac gccgaagagc cggtacacca tgtctcgatc
     6241 tttcaccggc cagggcgcct tgctcctcat gtagtagacg accccgtcgt cggactgctc
     6301 gagcaggcgc acctcggcaa gatcgggaat ccactcgtga aatcgcgagg tgtcggaaat
     6361 gaacgcacgc agatccgcaa gatcggtgca aacggcaacc tgagcacgat gggaccggaa
     6421 accggacggc atcatcagac cctcgacctg gataccgtgc accttccgta caggctccca
     6481 atcggcctgc gcggctgaag cggacgcggc ccagagcaaa aggacgatca gatacaggcg
     6541 caaaaccaca gccacatgaa aggacggcgc cgatggtatc agccgcaggg gactcaccgc
     6601 atccggcggc gagctcaccg atgaccgagg gcgcgccacg gcgcgcagca gctagcctgg
     6661 aagcagctct ttctcctgca gaagagcctc cagggccact agccagcttt cgtagtagcc
     6721 ccggctgggc tgctgagggt catcccgctc cgcatccgat atcgccgcga tgagcttctg
     6781 ctgaaactcc gaccactcga aagcgccctg ctcggccagg ctcaccgcca tggcgaaggc
     6841 acggcgttcc cactcatcgt gaaagaccat ttccccgctc ttgcgcggca gcgccgccgg
     6901 tgccggcatc tccgcgatga ggggcgcggc ttcccgctca ccggtcacgg cgcggcaacc
     6961 cgggtcacgc cgatcatcgc gtcgcgctcg acgagttcgg ccagctgatc ctcggtgagc
     7021 gtttcggtcc ccgtcggccg ctccgggagc accagatagc ggatctccgc gctgctgtcc
     7081 cacacgcgca cctccacgtc gtcgtccacg gcggtaccga actcctcgag cacgctgcgg
     7141 ggatcgatga ccacccgcga ccggtaagcc gggctcttgt accaggcggg cggcaggccc
     7201 aggacgggcc acggatagca ggagcagagc gtgcagacca cgacgttgtg aacctccggg
     7261 gtgttctcaa ccaccaccat atgctccccc tggagcccac cgtagccgag ttcgccgatg
     7321 gcggccgacg cgtcctccag caggcgctgc ttgaaggcgg cgtccaccca ggctcgagcc
     7381 accaccttgg caccgttcat gggtccgagg tcgttctcgt atatgtcggc aatgcggtcc
     7441 agcgcgtcct gcctgacgag ccctttctcc agcaacaggg attcgatacg accgacgcgc
     7501 tcttcgagcg cccggttttc atcgtctgag cgggaggccg catgataggg attcggggga
     7561 ccaccgtggt catggtcgtg gtcatggtcg ttgtcgtggt cattgctcat gagatctccc
     7621 ctccacgggc gacgcctcca gataatcttc ccagagatcg atgtggacgc agtcccttcg
     7681 cgcaaggttg tcgccccaca actcgtccgc ctcgaagcgc acgttgtaca gcggctgagg
     7741 attctcgccc agaccatggg ccgacgtgtc ggggaagaca tgcgtgccgt gaacggcgct
     7801 gacgacgccc cgctttcccc gaacgtaccg gggaaggcgg gtgtgtcccc gaacgtccag
     7861 attccgcgcc cggaccgagt cgcccacgtc gaaggtcggc cgtctgctca ccgggcgtgt
     7921 cgatggcgat ccggagcgaa ccatcgcgtc cacgaattcg ggatccagcg gcgggcggga
     7981 cggcgtggag gtgtgctcgc tcctcccgct ggcgatctca gcatccatca gcagtcccga
     8041 gtcccttccc aggatttcga tcccggcaag ccagtgctcg tagtaagacg tgctcagata
     8101 ggctgccgga tccatccgct cgatggcgtg ccgcgcctga tcgagggagc cgaactgccc
     8161 catgctgagc atgttcatcg cgaagacgcg gcgctcccag tccgagtgaa agacaggctc
     8221 gtcgtcttcc cgaacgatgg gcccgaagca cgtcatcccg ccgaggtcgt gaatgccgtt
     8281 caatgtgtcc tcctttagcc gccgacagca gacgggcgcg gtaaatgccc ggccactcag
     8341 tccaccagat agtactcgag cgtagatacc acgcggacgc gcttgatgtg cgggttgttg
     8401 ctgtcccggt cctgaatcga aaactgaccc tggcgggccg atcggatctt gccgagtacg
     8461 ctgtccgagt ccccagcaaa cttttccgca acgcgccggg cattccgggt tgcctcttcg
     8521 atcatttccg gcttgatgtc gttgaggcca ttgaagaggt actgcgtgcg caggtcgtag
     8581 ttgttctggg tcaccacgat gccctctttg ccgagctcta gcagccgccc gccagcctcc
     8641 cgcacggtcg cgaccgcttc ggtgaacacg gtgatcgtct gggccaccga gtagcggaag
     8701 cgggtgttgg gatctccgaa attctgggcc tgacgatcga gcacgttggg cgcgccgacg
     8761 ctgacttccc cgggcgtaaa gccgagatcg gcgagaaacc cgacgacccg cccgctctgc
     8821 ccctcgatct gcaccagaag cgcttgcaga tcgtccccag cggccacgat ctgcaccggc
     8881 cagatggcgg tgtctgccgg cacctcgcgc tcggccagac ccttgacctc cacgctgcgg
     8941 tctaaaaccc gcgcgtggac gaatccctga cctatcaggt agccggcgac ggcgaggccg
     9001 atggcaacga gcagcccggg aatgaaattg cccctcgaat cggtcatgcg tacgaaatcc
     9061 gcggcaagct ggcccgccat catccactat caggcggggt cgcttccag
//
LOCUS       DBNHQR010000079         1544 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_958010, whole genome shotgun sequence.
ACCESSION   DBNHQR010000079 DBNHQR010000000
VERSION     DBNHQR010000079.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_958010"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1325
                     /locus_tag="AC16VR_01750"
     CDS             <1..1325
                     /locus_tag="AC16VR_01750"
                     /inference="COORDINATES: protein motif:HMM:NF017743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR6009904.1"
                     /translation="RRRIREVEVAVTALGEDGLGEGQFEGRYVRIRNALPGETVTARV
                     LKRRAGALLAEAVEVRDRAPLRRAAPCTVFPRCGGCSLQHLEHGAQLRHKEKVLLDAL
                     AANGVVPQSVRRPVSGPRLHYRHKARLGVRCVGDNLLVGFRESFSSRVVRMADCRILA
                     EPLARCLPDLQSTLSSLRERHRVPQVELVAGDDSQAVIVRHLDPLGPESQVRLEEMSR
                     RLRMRVYLQAGGYDSLMLLGAPGKRPKLTYSIMEFGVTYRFDVADFVQVNPAINRALA
                     SSVAVALDPRPGITIADLFCGIGNFSLVLARHGAKVKGFEASAGAIERARENADLNGL
                     EHRCEFDLADLYDSGYAIPAGTQRLLLDPPRSGAGPHLARWLSGTELERVVYVSCNPM
                     TFASDAAVFEKQGFALIDVGVFDMFPQTAHVETLGVFVPSYSRTGASR"
     gene            1325..>1544
                     /locus_tag="AC16VR_01755"
     CDS             1325..>1544
                     /locus_tag="AC16VR_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009905.1"
                     /translation="MVKVGDSVPRHADGRIDILSWSENVCEDRSALDPARIRQAAELV
                     RQIEDAPDRYLETGVALGGLVADLMMDTP"
BASE COUNT          245 a          477 c          515 g          307 t
ORIGIN      
        1 gccgtcgtcg catcagggaa gtggaggtgg cggtaactgc tcttggcgag gatgggctgg
       61 gtgaagggca gttcgagggt cggtacgtga ggatccgcaa tgccctgcct ggcgagacgg
      121 tcaccgcgcg agtgctcaag cgacgcgccg gcgcgctgct tgctgaggcc gtcgaggtgc
      181 gcgatcgggc gccgcttcgg cgcgcggccc cctgcacggt ctttccgcgc tgcggtggct
      241 gcagtctgca acacctcgaa catggtgctc agctgcggca caaggagaag gtcctgctcg
      301 atgcgctggc agccaatggg gtggttcccc agagcgtgag aaggccggtc tccggtcctc
      361 ggcttcacta ccgtcacaaa gcccggctcg gggtccgctg cgtcggcgac aatctgctgg
      421 tgggatttcg cgaaagtttc agcagccgcg tggttcgaat ggcggattgc aggatcctcg
      481 ccgagccgct cgcgcgatgt ctcccggacc ttcagtcaac cctctcgagc ctgcgcgaaa
      541 gacaccgcgt gcctcaggta gagctggtcg ctggagatga ctcgcaggcg gtgatcgttc
      601 gtcatctgga tccgctcggt ccggaatccc aggtcaggct ggaggagatg tcgcgaaggc
      661 ttcggatgcg ggtctatctg caggccggtg gatacgactc cctcatgttg ctcggagcgc
      721 cagggaagcg gccgaagctg acctattcca tcatggagtt cggcgtcacc taccgttttg
      781 acgtggcgga tttcgtgcag gtgaaccctg cgatcaatcg cgcgctcgcg tcgtccgtag
      841 cggtcgcctt ggacccgcgg ccgggaatca ccatcgccga tctcttctgc ggcataggca
      901 atttttctct ggtgctggcc cggcatggcg caaaggtcaa gggctttgag gcgtctgccg
      961 gcgctatcga gcgggcccgg gaaaacgcgg acctcaatgg tctcgagcac cgatgtgagt
     1021 ttgacctagc cgatctgtat gattcgggct atgcgatccc cgccggcacc caacggcttc
     1081 ttctcgaccc accgcgttcc ggtgccggtc cccacctcgc ccgttggctg agcggcacgg
     1141 agctcgagcg ggtggtctac gtttcctgta accccatgac gtttgccagc gatgccgccg
     1201 tcttcgagaa gcaaggtttc gcgctgatcg acgtgggcgt ctttgatatg ttcccgcaga
     1261 cggcgcacgt ggaaaccctc ggggttttcg tcccctctta ctcgcgcacc ggggcatctc
     1321 gctgatggtc aaggtaggcg acagcgttcc ccggcacgcc gatggccgca tcgatatcct
     1381 gagctggtct gagaacgtct gcgaagaccg ctctgcgctc gacccggctc gaatccgaca
     1441 ggcggcggag ctcgttcggc aaatcgagga cgcgcccgac cggtatctgg aaaccggcgt
     1501 cgccctgggt ggcctggtag cggatctgat gatggatacc ccca
//
LOCUS       DBNHQR010000080         8927 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1464747, whole genome shotgun sequence.
ACCESSION   DBNHQR010000080 DBNHQR010000000
VERSION     DBNHQR010000080.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8927)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8927)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8927
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1464747"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1870)
                     /locus_tag="AC16VR_01760"
     CDS             complement(<1..1870)
                     /locus_tag="AC16VR_01760"
                     /inference="COORDINATES: protein motif:HMM:NF025056.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HIR6009906.1"
                     /translation="MADIFISYARSTRRQAEAIGEALVELGYSIWRDDALPAHRAYAD
                     EIQKQLNIAKAVVVVWSADAIKSDWVRSEADRARNDRKLVQLTVDGAALPMPFDQIQC
                     ANLAGWRGEGRAAGWATVLESIRALTDTAGKESPVSAGTQSQQQPEPILAVLPFDNLS
                     TDPEMQFFSDGVSEEIIQRLSRGASLKVIGRTSSFQFRGERKAEAASALGCSHILDGS
                     VRRGAGRMRLSAHLVEASSGTTLWSERYDRSLEDVFAVQDDVSENIAGALHHTFQSFS
                     TPAVDPGAYDLYLRASPESYAPEEMRTNIGLLEVATQRAPDFPEAWGRLAYLRAFLRF
                     YEPFPGRGASAAIIARESAHALQADPENGDALAARAFVVAPFGNFIELDTAVERLRSA
                     PGDFDARKFAGWLLRNFGRVRESLAEDERAYLGNALDPMCANTLALARLAAGRVAEAL
                     PVLENLVERVPDMVFPFANLLRAQALLENWEALDELLELNADRPLREFQDTLDFIRTK
                     RNPSAENIAVWKSQFEAHIARTGCIDISRVVYAAHLGLVDEAYQATEQAHLGPTGTNE
                     DVMGPDGYRTSLLFQAAMPELRNDPRFVPLCARLGLVEFWTSTGKWPDCADEVPYDF"
     gene            complement(1962..2381)
                     /locus_tag="AC16VR_01765"
     CDS             complement(1962..2381)
                     /locus_tag="AC16VR_01765"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR6009907.1"
                     /translation="MAASLEDNPMPVNLIDYMPFARVLGLEVTHSDKDKVTASIEVSE
                     DLCTTGKIMHGGAVMAVADTLGAIGAYLNLPDGAKATTTIESKTNFLGAARLGTRVLA
                     ETTPVHVGGRTSVWQTRLTGEDGKPVALVTQTQLVLS"
     gene            complement(2396..3400)
                     /locus_tag="AC16VR_01770"
     CDS             complement(2396..3400)
                     /locus_tag="AC16VR_01770"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6009908.1"
                     /translation="MRFSLMLPVFVDAGAASPFERTLGFARTADELGYHMGTFGHHSF
                     TPQIADPSAPFALLSAVAARTERLRLGTGVYLAPLHHPVTVAEQAAMLDQISGGRAVL
                     GVGLGYRPYEYEGFGLDFHTRGKRLDELLTAIREGWTRGEFRHDGEHFRIPPSPVNPP
                     CVQKPQVPILVGGSAPAAITRAARLGDGWFALPQEGLEIMRRQVDAYRAACREAGRTP
                     YVCLMRNAWIAPTMDQVEAEWLPRMIEFHKTFADARADRSDTVIERLLAGETFTLDEY
                     IRARAIAGTPEVCIAEIERWQAAIQPDEYSLIFGGSDDAARLEDAVTLFAREVMPAFS
                     "
     gene            complement(3418..4029)
                     /locus_tag="AC16VR_01775"
     CDS             complement(3418..4029)
                     /locus_tag="AC16VR_01775"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6009909.1"
                     /translation="MKFYNSIGPNPQVVRTFAAERGIELDMEEVDLMAADNRREPYLS
                     KNPAGQSPCLELDNGDYVSEVTAICEYLDETSPGTSLIGSTPEERAETRMWVRRIDLN
                     ICEPLGYGFRFGEGLDFFKDRITTLPEASEGMKRIAAEKIAWLDGLMAGREFVCGDRI
                     TLADVLLYCFLAFGKTVGQPYAETNQNIHAWFERMSQRPSVSA"
     gene            4279..5739
                     /locus_tag="AC16VR_01780"
     CDS             4279..5739
                     /locus_tag="AC16VR_01780"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6009910.1"
                     /translation="MTKGYQVRLPTKIVYGLGSVSDGVKNTTFNVFLLFFYTQVAGLS
                     GSLAGAAILIALVFDAVSDPLIGNISDNFHSRWGRRHPFMYAAALPMAVFFGLIFSPP
                     QGLGQAGLFAWMLTMAIGVRVSMTLYAIPSGALVAEMTSHYDERTSLVSFRVLSGWLG
                     GLVASQMGYLVFFAPSAAYADGRLDPQAYGDYAVFGAITIFCAILICAIGTHRLIPEL
                     KSPPAHTPFTLRRFGVELREVFSNRPYLVLVFTVLVVSTATGFTDVMGLYVNTYFWEF
                     TTAQLAIIVFGALAGVVAAFSVTATLAQRTDKRTMALAMLLVVVIIGPLPVVLRLLGL
                     MPANGDPLLLGIMIAWTGVIVFVAVGVTILAGSMIADTVDLNELRTGKRQEGMFSAAM
                     GLTAKATSGIGGFVGGVVLDVVDFPTGVAAGEVDADTLFALGVAVGPGMFLLWITVLI
                     ILSRYRLSRADHADILGKLEARRNAAEQPGGEAAGG"
     gene            5813..7153
                     /locus_tag="AC16VR_01785"
     CDS             5813..7153
                     /locus_tag="AC16VR_01785"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6009911.1"
                     /translation="MSEAVDARSPGTTYQELLDTDSHAVPDVLRLESPAPRRLNDLSI
                     DRYTSRDFHDREMEKLWSRVWQFACREEHIPNPGDYLVYEIGRHSYVVVRSHQGAIKC
                     FVNACLHRGRMLKHYDGNCTELRCPFHGFAWETDGRLKDIPASWDFPQIVASEFSLPE
                     AQVATWAGFVFVNPDPDAAPFREFLGGMAAHFDRWNLADRYVQVHVIKEIRANWKIAQ
                     EAFCEAFHVNATHPQTMPYLGDTNSQVDVWDTFSRVITPGGTPSPLLDWEPDESDILR
                     HMLDIREDMELPWSLEGTTARKSAADSARERWRPVVGGLVDEWSDAEFIDNLDYTLFP
                     NFHPWGAFNRIVYRFRPNGDDHRSSLMEVMMLAPFTGERPPPADVVRLDADQPWTDAS
                     ELGMLAKVFEQDTFNMGRVQRGLETTRKPGVTLAGYQEAKVRWLNETLDEWLER"
     gene            7156..7365
                     /locus_tag="AC16VR_01790"
     CDS             7156..7365
                     /locus_tag="AC16VR_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009912.1"
                     /translation="MKGMKHPFGRALYEQDGNGNVLVTTVDGVWGRFRTDGSWLEGEL
                     KEADPHMCGWVGGPTVAHHRIQVEE"
     gene            complement(7403..7897)
                     /locus_tag="AC16VR_01795"
     CDS             complement(7403..7897)
                     /locus_tag="AC16VR_01795"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6009913.1"
                     /translation="MSDQAFTPEEIAELRNLLEIEKIRKVKLLYSQLMDSRDIDALAD
                     IFAEDAICEFGPYGTWHGQDEIRANYHQVFKDAAPYDGIHATTNQWVELTSPTTAVSR
                     TYLIDVSHEANPRVNPVVWYGLYDEDYEKIEGAWKVKRCSLQFLWPERLVNEGWPGPF
                     PPAS"
     gene            complement(7937..>8927)
                     /locus_tag="AC16VR_01800"
     CDS             complement(7937..>8927)
                     /locus_tag="AC16VR_01800"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6009914.1"
                     /translation="AFYNTCSHRGAPVVTEPEGKRPRLVCKYHGWTYSHDGELLAIRD
                     PEDFRDLDFSCRGLRPIRCESFGNLIFVNFDDQAPTLLEWLGPVAAEWEEFQFDKCRL
                     ASRHIFDLNCNWKIAMEANTEVYHVRSIHPKTVAPMLDDRRNVNTLYPNGHGRMVAPV
                     PPSAKVPSIVDLPSGSSIETVGEIARTCTQSYGVFPNWVSPLNHRVLPPLLFWPNGID
                     KCRLETWTLAPDWGNEPKPDMWTVNDGESLSDVLLEDTEFGEWIQKSVESYGFKGVPL
                     SYQEARIYHWNQFADRMIGIDNIPPELRVAQVIGEEWVWPNEPRLALARASAG"
BASE COUNT         1412 a         2911 c         3011 g         1593 t
ORIGIN      
        1 ggaagtcgta gggcacctcg tcggcgcaat ccggccactt gccggtggat gtccagaact
       61 ccaccagccc cagccgggcg cacaggggta cgaagcgcgg gtcgttgcgc agctcgggca
      121 tggccgcctg aaacagcagc gacgtgcggt agccgtccgg gcccatgacg tcttcgttcg
      181 tgccggtggg tcccaggtgg gcctgctcgg tggcctggta ggcttcgtcg accaggccga
      241 gatgggcagc gtagacgacg cgggagatgt cgatgcagcc tgtcctcgcg atgtgcgcct
      301 cgaactggct cttccagacg gcgatgttct ccgccgacgg gttccgcttg gtgcgtatga
      361 agtccagagt gtcctgaaat tcccgcaagg gacggtcggc attgagctcg agcagctcgt
      421 ccagcgcctc ccagttctcg agcagggcct gggcacgcaa caggttggcg aaaggaaaaa
      481 ccatgtcggg aacgcgctcg accagattct ccaggaccgg cagcgcttcc gcgacgcgtc
      541 ccgctgccag tcgggccaga gccagagtat tggcgcacat ggggtcgagg gcgttgccga
      601 ggtaggcgcg ctcgtcctcg gcgaggcttt cgcgaacccg gccgaagttc ctcagcagcc
      661 aaccggcgaa ctttctcgcg tcgaagtctc cgggcgcgct gcgcaggcgc tcgacggcag
      721 tgtcgagctc gatgaagttt ccgaaaggcg cgaccacgaa tgccctcgcg gccagggcgt
      781 cgccgttctc gggatccgcc tgtaaagcat gggccgattc tctggcgatg atcgcggcgc
      841 tggcgccgcg cccgggaaac ggttcgtaga accgcaggaa ggctcgcagg taggcgagcc
      901 ggccccaggc ctcgggaaaa tcgggcgcac gctgggtggc aacttcaagc aggccgatgt
      961 tcgtgcgcat ttcctcgggc gcgtaggatt cggggctcgc ccgaaggtaa aggtcgtagg
     1021 cgcctgggtc cacggcgggg gtcgagaaac tctgaaacgt gtgatgcagc gccccggcga
     1081 tgttctcgga aacgtcgtcc tggacggcga acacgtcctc cagggaacgg tcgtagcgct
     1141 ccgaccacaa cgtggtgccc gaggacgcct ccacgaggtg cgcgctcagg cgcatccgcc
     1201 ccgcgccgcg tcgaactgaa ccgtccagga tgtggctgca gccgagtgca gaggcggctt
     1261 ccgccttgcg ctccccgcga aactgaaagc tcgacgtccg gccgatgacc ttgaggctcg
     1321 ccccccggga cagccgctga atgatctcct cgctgacccc gtcggagaaa aactgcatct
     1381 ccggatcggt ggagaggttg tcgaagggca gaacggcgag gatgggctcc ggctgctgct
     1441 gcgattgcgt gcccgcagac accggtgatt ctttcccggc tgtatcggtg agcgcacgaa
     1501 tgctctccag caccgtcgcc cagccggcgg cccggccttc tcccctccag ccggcgaggt
     1561 tggcgcactg aatctgatcg aacggcatgg gcagcgctgc cccgtcgacg gtcagctgga
     1621 cgagcttgcg gtcgttgcgc gcacggtcgg cttcggagcg cacccaatcc gacttgatcg
     1681 catccgcgga ccagacgact accaccgcct tggcaatgtt gagctgcttt tggatctcgt
     1741 cggcgtaggc gcgatgggcg ggcagggcgt cgtctcgcca gatggagtaa ccgagctcta
     1801 ccagggcctc gccaatggcc tctgcctggc gccgggtcga ccgcgcgtac gagatgaaga
     1861 tgtcggccac taacgctcga cctggtgaca ggagcgacgg cgagtataac gggaaaccag
     1921 aaaccggttg ggcagaccca agtactgccg ccgtcagccg atcagctcaa aaccaactgc
     1981 gtctgggtga cgagggcgac gggcttgccg tcctcgccgg tgaggcgcgt ctgccacacg
     2041 gaggtgcgcc cgcccacatg gacgggcgtc gtctccgcca ggactcgggt gcccagacgc
     2101 gccgcaccta gaaagttggt cttgctctcg atggtggtgg tggccttggc gccgtcgggc
     2161 aggttcaggt aggcgccgat ggcgccgagg gtgtccgcca ccgccatcac ggccccgccg
     2221 tgcatgatct tgccggtggt gcacagatcc tcgctcacct cgatggatgc ggtcaccttg
     2281 tctttgtccg aatgggtcac ctcgagcccc agcacgcggg cgaagggcat gtagtcgatc
     2341 aggttcacgg gcatggggtt gtcctctaat gacgccgcca tccgggcgtc cgtgttcagc
     2401 taaacgccgg catgacctcg cgggcgaaca gggtcaccgc gtcttcgagc cgcgcggcgt
     2461 cgtccgagcc gccgaagatg agcgagtact cgtcgggctg gatggccgcc tgccagcgct
     2521 cgatctcggc gatgcagacc tccggcgtgc cggcgatggc gcgcgctcgg atgtactcgt
     2581 ccagcgtgaa cgtctccccg gccagcaggc gctcgatgac ggtgtcggac cgatcggccc
     2641 gggcatcggc gaaggttttg tggaactcga tcatccgggg cagccattcg gcttccacct
     2701 ggtccatggt gggcgcgatc caggcgttgc gcatgaggca gacgtagggt gtgcgcccgg
     2761 cttcccgaca cgctgcgcgg taggcgtcca cctggcgccg catgatctcc aggccttcct
     2821 ggggcagggc gaaccagccg tctcccagac gggcggcccg ggtgatggcg gcgggtgccg
     2881 agccacccac cagaatcggc acctggggct tctgcacgca gggcgggttc accggcgacg
     2941 gcggtatgcg gaagtgctcg ccgtcatggc gaaattcgcc ccgcgtccat ccttctctga
     3001 tcgcggtgag cagctcgtcc agccgctttc cgcgggtgtg gaaatcgagc ccgaagccct
     3061 cgtactcgta gggccggtag ccgaggccga cccccagcac cgcgcgcccg ccggatatct
     3121 ggtccagcat ggcggcctgc tctgcaacgg tcaccggatg gtgcagcggg gcgaggtaga
     3181 cgccggtgcc caggcgcagc cgctcggtac gcgccgccac cgctgacaga agcgcaaacg
     3241 gggcggacgg atcggcgatc tgtggcgtga acgagtgatg gccgaaggtg cccatgtggt
     3301 agccgagctc gtccgcggtg cgggcgaagc ccagtgttcg ttcgaagggg ctcgcggccc
     3361 cggcgtccac gaacaccggc agcatgagac tgaacctcat gggcgcttac tcgggaatca
     3421 cgccgacacg cttggccgct gagacatccg ctcgaaccac gcgtggatgt tctgattggt
     3481 ctccgcgtag ggctggccca cggtcttgcc gaaagcgagg aagcagtaca gcagcacgtc
     3541 ggccagcgtg atcctgtcgc cgcagacgaa ctcgcggccg gccatgaggc cgtcgagcca
     3601 ggcgatcttc tccgccgcga tgcgcttcat gccttcggaa gcttccggta gggtggtgat
     3661 gcggtccttg aagaaatcca atccctcgcc gaagcggaag ccgtagccca ggggctcgca
     3721 gatgttcagg tcgatgcggc gcacccacat gcgcgtttcg gcgcgctcct ccggcgtgct
     3781 gccgatcaga gacgtgcctg ggctcgtctc gtcgagatac tcgcagattg ccgtgacctc
     3841 gctgacgtag tcgccgttgt cgagctcgag gcacggcgac tggccggccg ggttcttcga
     3901 caggtagggc tcccggcggt tatcggccgc catgaggtcg acctcctcca tgtcgagttc
     3961 gatgccgcgc tcggcggcaa acgtgcgcac cacctgaggg ttcgggccga tggagttgta
     4021 gaatttcatt gcgtgttacc cgattgattc aaacgtcgga ttctaggggc cggcgccgct
     4081 gcgcaccacc acgggcgacc ggcgcgccgc cggcctggag gctgatgccc gggttgtacg
     4141 ggccgcgcgg gcaggtgcaa gcaccgttcc aggcgcgtag actcgccgac cttccccgga
     4201 gcccggattc tgccgatagc cgtgtgcccg ggtccgcccc cggcggggcc gtgatattga
     4261 ggcatcgtgg aaggtcccgt gacgaaaggc tatcaggtca ggcttcccac caagatcgtc
     4321 tacgggcttg gcagcgtttc cgacggcgtc aagaacacca cgttcaacgt ttttctgctc
     4381 ttcttctaca cccaggttgc gggcctctcc ggctcgttgg ccggtgcggc tatcctcatc
     4441 gcgctggtgt tcgatgcggt cagcgacccg ctcatcggca acatctcgga caactttcac
     4501 tcccgctggg gccgccgcca tccgttcatg tatgccgccg cgctgcccat ggccgtcttt
     4561 ttcgggctga tcttcagccc gccgcaaggg ctgggccagg ccggactctt cgcctggatg
     4621 ctgaccatgg ccattggtgt ccgggtgtcc atgacgttgt acgccattcc cagcggcgcg
     4681 ctggtggcgg agatgacgag ccactacgac gagcgaacgt ccctggtcag cttccgggtg
     4741 ctctccggct ggctgggagg gctggtcgcc tcccagatgg gctacctcgt cttctttgcc
     4801 ccgtctgccg cctacgcgga cggtcgcctg gatcctcagg cttacggcga ctacgccgtc
     4861 ttcggcgcca tcacgatctt ctgcgccatc ctgatctgcg cgatcggcac ccatcgattg
     4921 attcccgaac tgaaatcgcc gcccgcgcat acaccgttta ccctgcgccg gttcggcgtc
     4981 gagctgcgcg aggtgttcag caaccgcccg tatctcgtgc tcgtgttcac ggtgctcgtc
     5041 gtctccacgg ccaccggctt caccgacgtc atgggtctgt acgtgaacac gtacttctgg
     5101 gagttcacca ccgcgcagct tgccatcatc gtcttcggcg cgctggccgg cgtggtagcg
     5161 gccttctcgg tcacggcaac gcttgcgcag cgaacggaca agcgcaccat ggcgctggcg
     5221 atgctgctgg tggtggtgat cattggacct ctgcccgtgg tgctgcggct gctgggcctc
     5281 atgccggcga acggcgatcc gctgctgctc ggcatcatga tcgcctggac gggcgtgatc
     5341 gtgttcgtcg cggtgggcgt gacgattctg gccggctcca tgatcgccga caccgtggac
     5401 ctcaacgagc tgcgcacggg caaacgccag gaaggcatgt tcagcgcggc catgggcctg
     5461 acggccaagg cgacatcggg catcggcggc ttcgttggcg gtgtggtcct ggacgtggtc
     5521 gactttccga cgggcgtggc cgcgggcgag gtggatgcgg ataccctctt cgcgctgggc
     5581 gtggccgtgg gccccggcat gtttctgctc tggatcacgg tgttgatcat cctgtcgcgc
     5641 tatcggctga gccgggccga tcacgcggac atcctgggca agctcgaagc ccgccggaac
     5701 gccgccgagc agcccggcgg ggaagccgcc gggggctagc cgagcgctgc tttgcattcc
     5761 gcccagcgcc ggtggcacac tcggcggtca ctcagatcaa aggaaggccg ccatgtcaga
     5821 ggccgtcgac gcccgctcgc cgggaacgac ctatcaggag ctgctcgaca ccgactccca
     5881 cgccgtgccg gatgtccttc ggctggagag cccggcgccg cgccggctca acgacctgtc
     5941 catcgaccgc tacacctccc gcgactttca cgatcgggag atggagaagc tctggtcgcg
     6001 ggtctggcag ttcgcctgcc gcgaggagca catccccaac ccgggcgact acctggtcta
     6061 cgagatcggc cggcactctt acgtggtggt gcgttctcat cagggcgcga tcaaatgctt
     6121 cgtaaacgcg tgccttcacc ggggccgcat gctcaagcac tacgacggca actgcaccga
     6181 gctgcgctgc ccgtttcacg gcttcgcctg ggaaaccgac gggcggctga aggacatacc
     6241 ggcgagctgg gacttccccc agatcgtcgc ctccgagttc tctctgccgg aagcccaggt
     6301 agccacctgg gccggtttcg tcttcgtcaa ccccgacccg gacgcggcgc cgttccggga
     6361 attcctgggc ggcatggccg cccacttcga tcgctggaac ctcgccgacc gctacgtgca
     6421 ggtccatgtg atcaaggaga ttcgggcgaa ctggaagatt gctcaggagg ccttctgcga
     6481 ggccttccac gtcaacgcca cgcacccgca gaccatgccc tatctgggcg acaccaacag
     6541 ccaggtggac gtgtgggata cgttttcccg ggtgatcacg ccgggcggca cccccagccc
     6601 gctgctcgac tgggagcccg acgagagcga catcctccgg cacatgctgg acatccgcga
     6661 ggacatggag ctgccctggt ccttggaggg aaccaccgcg cgcaagtcgg cggccgattc
     6721 cgcgcgagag cgatggcggc ccgtggtcgg cggtctggtg gacgagtggt cggacgctga
     6781 gttcatcgac aacctggact acaccctgtt tcccaatttt cacccctggg gcgccttcaa
     6841 ccgcatcgtc taccgcttcc gccccaacgg cgacgatcac cggtcctcgc tgatggaggt
     6901 gatgatgctg gcgcccttca ccggtgagcg cccgcccccg gcggacgtcg tccgcctcga
     6961 tgccgaccag ccgtggacgg acgcatcgga gctgggaatg ctggccaagg tgttcgagca
     7021 ggacacgttc aacatggggc gcgttcagcg gggcctggag accacgcgca agccgggcgt
     7081 cacgctcgcc ggctatcagg aagccaaggt gcgctggctc aacgagaccc tggacgaatg
     7141 gctggagcgc tgaacatgaa aggcatgaaa catccgtttg gtcgggccct gtacgagcag
     7201 gacggcaacg gcaacgtgct ggtgaccacg gtggacggcg tgtgggggcg gttccgtacc
     7261 gacggcagct ggctcgaggg cgagctcaaa gaggccgacc cgcacatgtg cggctgggtg
     7321 ggcgggccca cggtggccca ccatcggatt caggtggagg aatgagcggc gccgccccag
     7381 cgtcgcgccc gcaccggtcg ggctagctgg ccggcgggaa cggccctggc cagccctcgt
     7441 tgacgagccg ctccggccag agaaactgca ggctgcagcg cttcaccttc caggcgcctt
     7501 cgatcttctc gtagtcctcg tcgtaaagac cgtaccagac caccgggttc acccgggggt
     7561 tggcctcgtg gctgacgtcg atgaggtacg tgcggctcac cgccgtggtg ggactggtca
     7621 gctccaccca ctggttggtg gtggcgtgga tgccgtcgta gggcgcggcg tccttgaata
     7681 cctggtggta gttcgcgcgg atctcgtcct ggccgtgcca ggtgccgtag ggcccgaatt
     7741 cgcagatcgc gtcctcggca aagatgtcgg cgagggcgtc gatgtcgcgg ctgtccatca
     7801 gctgcgagta gagcagcttc actttccgaa tcttctcgat ctccaggaga tttctcagct
     7861 ccgcgatctc ctcgggggta aaggcctggt cagacatctc ttcgtgctcc tgtgttgagt
     7921 gcgcgcccga tggccgtcag ccggcgcttg cccgggcgag cgcgagtcgg ggctcgttcg
     7981 gccaaaccca ctcctcgccg atcacctggg ccacgcgcag ctccggcgga atgttgtcga
     8041 tgccgatcat gcggtcggcg aactggttcc agtggtagat cctggcctcc tggtagctca
     8101 gcgggacccc tttgaagccg taggactcca ccgacttctg gatccactcg ccgaactcgg
     8161 tgtcctccag cagcacgtcg ctgagactct cgccgtcgtt caccgtccac atgtcgggtt
     8221 tcggctcgtt cccccaatcc ggcgccaggg tccaggtctc cagccggcac ttgtcgatgc
     8281 cgttgggcca gaacagcagg ggagggagca cccggtgatt caggggtgac acccagttgg
     8341 gaaagacgcc gtaggactgg gtgcaggtgc gggcgatctc gcccacggtc tcgatgctgc
     8401 tgccgctcgg caggtcgacg atggacggca ccttggcgct gggcggcacc ggcgccacca
     8461 tccgtccgtg cccgttggga tacagcgtgt tcacgttgcg gcgatcgtcg agcatgggcg
     8521 cgacggtctt cgggtggatg ctgcgaacgt ggtagacctc cgtgttcgcc tccatggcga
     8581 tcttccagtt gcagttcagg tcgaagatgt ggcgggaggc gagccggcac ttgtcgaact
     8641 gaaactcctc ccactccgcg gcgaccgggc ccagccactc cagcagcgtg ggggcctggt
     8701 cgtcgaagtt cacgaagatc aggttgccaa agctctcgca gcggatgggc cgcaggccgc
     8761 ggcaggagaa atccagatcg cggaagtcct cgggatcgcg gatggccaga agctcgccgt
     8821 cgtggctgta ggtccagccg tgatatttgc acaccagacg cgggcgtttg ccttccggtt
     8881 cggtgacgac gggcgcgccg cggtggctgc acgtgttgta gaaggcg
//
LOCUS       DBNHQR010000081         4317 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_514402, whole genome shotgun sequence.
ACCESSION   DBNHQR010000081 DBNHQR010000000
VERSION     DBNHQR010000081.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_514402"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="AC16VR_01805"
     CDS             <1..252
                     /locus_tag="AC16VR_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIR6009915.1"
                     /translation="NVEGELPGPKNYFNLIFRRARMQGFLAPDYAPRFDEAYAELQGW
                     LAEGKIRQRETVIEGFENLPKALVLLFEGANTGKMMVKV"
     gene            complement(310..1191)
                     /locus_tag="AC16VR_01810"
     CDS             complement(310..1191)
                     /locus_tag="AC16VR_01810"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6009916.1"
                     /translation="MKFGLSMFTTDYSASPTEVAKAAEANGFESFWVSEHSNIPVDTD
                     FPMSDTVPREYSSMLDPFVALAAAATVTERINLGTAICLVTQRDPINCAKLVASIDQL
                     SGGRMLFGIGAGWNEPEMRNHGTDPNTRFRLMRERVEAMKALWTNEVAEYHGRLVNIE
                     PTWQWPKPVQQPHPPVIVAGAGPNVLKRVIAYGEGWLPVVTPVAPEQMKGRVTPMDEF
                     VEMAGELERMADAAGRPTPAITVSGMNLDAATFDAFAELGVERMILRLTTAPLDDVLA
                     QIEQHVETVRSAGGSLH"
     gene            complement(1231..3183)
                     /locus_tag="AC16VR_01815"
     CDS             complement(1231..3183)
                     /locus_tag="AC16VR_01815"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR6009917.1"
                     /translation="MSELTHLFQPIQIGTMNLRNRIMMPPHGGFIGDLWGSEEAAEQN
                     LAYWARRARDGVAWIDGITGFVDNTLMVPGFLPTGLGAITRGVFRMPHFKERAGRYAD
                     VIHEGGACATSQLVLQGGMPHGPSARLANYTNNSVPHVLDKDEIRWLVEEYAYSARQI
                     ADAGLDGLELHANHEDLLELFLSPATNHREDEYGGDLNGRLRFIKEILEAIRAEVGNS
                     ITVGVRLNMDEFFEGGYDLDEGLEIAKGLDATGHVDYFHGVIGNNWGAPSYIQPHHYG
                     LANWSEMAGRFKAAVGVPVVYAGRVTTPEAGEAVLAAGHADVVGMARAMFADGEIVSK
                     SKAGRLDEIRPCIGCNDCRHAGVVEGLPFGCTVNPRTGYEHKPPPAPASPPKNLLVVG
                     GGPAGLELAAAAAERGHTVTLWERDDALGGQMRLAAQVPENRAFGDFIEFQERRLARL
                     GVTVETGREATAESIAEAGFDVVAIATGATPRGHEASGAHLPFVLEGRDVMSGEAKPG
                     KRVAVIAMEDHLQPLTVASFLADRGHEVQLIYQTPSIAPLIGKYSIGAPLAKITASGA
                     RVRVMERVTSIEQGRIETANVYSGAGSEITDFDSVVLACGGVADSGLYHSLKGKVDEL
                     HVLGDAYAPRRIWFATRQAYALAATL"
     gene            3284..3925
                     /locus_tag="AC16VR_01820"
     CDS             3284..3925
                     /locus_tag="AC16VR_01820"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR6009918.1"
                     /translation="MSEEPEDVQIHLDSVDHVLSTARSVRRKLDFSRPVDPEVIFDCI
                     NVSTQAPTGRGGESWRFLVVTDLGRKREIAGLYRDVLVGLQQERGLVIKPTQQALVDR
                     LHEIPAMIFVCSMGAPPGPEIPTQVAYYGSVLPSAWSLMLSLRARNLGATWTTLLSSQ
                     QEALGRILDVPEDVVFTVMLPVGYMKGARLKKADRMDARRVTFWNRWGNDAPH"
     gene            complement(3941..>4317)
                     /locus_tag="AC16VR_01825"
     CDS             complement(3941..>4317)
                     /locus_tag="AC16VR_01825"
                     /inference="COORDINATES: protein motif:HMM:NF025134.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HIR6009919.1"
                     /translation="QLPDDPQHPAVRWVPKAWKLTTWGVVMFDRGHERAHIHPNGWLS
                     GVFYLELPDLIADPARKPEGWLEFGRPTTDLHVQSELTLRHYQPAYGQMFLFPSYFYH
                     GTVPFRSDQERVCVAFDVEPLY"
BASE COUNT          661 a         1426 c         1484 g          746 t
ORIGIN      
        1 aacgtggaag gagagctgcc ggggccgaag aactacttca acctgatctt ccggcgggcg
       61 cgcatgcagg gctttctggc cccggattac gccccgcgct tcgacgaggc ctacgccgag
      121 ctgcagggct ggcttgccga aggcaagatc aggcagcgcg agactgtcat cgaaggcttt
      181 gaaaatctgc cgaaagcgct ggtgctgctt ttcgaaggtg ccaacaccgg caagatgatg
      241 gtgaaggtct agcgccgctc agccgtcgcc ggtcgttcct cccggccccg gcacccgggc
      301 gtcgacgttt cagtgcagtg atcctcccgc ggagcgcacc gtctcgacgt gctgctcgat
      361 ctgggcgagc acgtcgtcca ggggggcggt ggtcagcctc agaatcatcc gctccactcc
      421 cagctcggcg aacgcatcga acgtggcggc atccaggttc atgcccgaga ccgtaatggc
      481 gggggtgggg cgcccggctg cgtccgccat gcgttccagc tcgccggcca tctccacgaa
      541 ctcgtccatg ggcgtcacgc ggcccttcat ctgctcgggt gccacgggcg tgaccacggg
      601 cagccagcct tcgccatagg cgatcacccg cttgagcacg ttgggcccgg ccccggcaac
      661 gatgacgggc gggtggggtt gctgcacggg tttcggccac tgccaggtgg gctcgatgtt
      721 cacgagacgc ccgtggtact cggcgacctc gttggtccac agggctttca tggcctccac
      781 ccgctcccgc atcaggcgga agcgcgtgtt tggatccgtg ccgtggttgc gcatctccgg
      841 ctcgttccat cccgcgccga tgccgaacag catgcgcccg ccggagagct ggtcgatgct
      901 ggccacgagc ttcgcgcagt tgatgggatc ccgctgagtc accaggcaga tggccgtgcc
      961 gagattgatg cgctcggtga ccgtcgccgc ggcggccagg gcgacgaagg gatccagcat
     1021 gctggagtac tcgcggggca cggtatccga catcggaaag tccgtgtcca cagggatgtt
     1081 ggagtgctcg gacacccaga acgactcgaa cccgtttgcc tccgcggcct ttgcgacttc
     1141 ggtggggctc gcgctgtagt ccgtggtgaa catgctcagg ccgaatttca tggcgttggt
     1201 ctccttgtcg gaagcgtgcc cgccgaacgg tcagagggtt gctgccaggg cgtaggcctg
     1261 ccgcgtggca aaccagatcc tccgcggcgc gtaggcgtcg ccgagaacgt gcagctcgtc
     1321 gaccttgcct ttgagcgagt ggtacagccc gctgtccgca acgccgccgc aggcgagcac
     1381 cacggagtcg aagtcggtga tctccgagcc ggcgccggag tagacgttgg cggtctcgat
     1441 ccggccctgc tcgatgctgg tgacccgctc catcaccctc accctggcgc cggatgcggt
     1501 gatcttggcc aggggcgcgc cgatggagta cttgccgatg agcggggcga tgctgggcgt
     1561 ctgatagatg agctgcacct cgtggccccg atcggccagg aagctggcaa ccgtgagggg
     1621 ctgcagatgg tcctccatgg cgatgacggc cacccgcttg cccggcttgg cctctcccga
     1681 catcacgtcg cggccctcca ggacgaaggg caggtgggcg cccgaggcct cgtggccgcg
     1741 cggcgtggcg ccggtggcga tggccaccac gtcgaagcct gcctcggcga tggattcggc
     1801 ggtggcctcc cggccggtct ccacggtgac gcccagccgg gcaaggcggc gctcctgaaa
     1861 ttcgatgaag tctccgaagg cccggttctc gggcacctgg gcggcaaggc gcatctgccc
     1921 gccaagggcg tcgtcgcgct cccacagggt gacggtgtga ccgcgctcgg cggcagcggc
     1981 tgccagctcc aggccggccg gccccccgcc caccaccagc aggttcttag gcgggctcgc
     2041 cggcgccggc ggcggcttgt gctcgtagcc cgtgcggggg ttgaccgtgc agccgaatgg
     2101 caggccctct accacgcccg cgtgccggca gtcgttgcag ccgatgcagg gacgaatctc
     2161 gtccagacgc ccggccttcg acttggagac gatctcgccg tcggcgaaca tggcgcgggc
     2221 catgcctacc acgtcggcgt ggccggcggc caggacggcc tccccggctt cgggcgtggt
     2281 gaccctgccc gcgtagacca cgggcacgcc cacggcggcc ttgaagcggc cggccatctc
     2341 gctccagttg gcaaggccgt agtggtgcgg ctggatgtag ctgggcgcgc cccagttgtt
     2401 gccgatcacc ccgtggaagt agtccacgtg gccggtggcg tcgaggcctt tggcgatctc
     2461 gagcccttcg tcgaggtcgt agccgccttc gaagaactcg tccatgttca ggcgcacgcc
     2521 cacggtgatg gagttgccca cctcggcccg gatggcctcc aggatctcct tgatgaagcg
     2581 cagacgtcca ttcagatcgc cgccgtactc gtcctcccga tgattggtgg ccggggaaag
     2641 aaacagctcc agcaggtcct cgtggttggc gtgcagctcc aggccgtcca ggccggcatc
     2701 cgcaatctgc cgggccgagt aggcgtactc ctccaccagc cagcggatct cgtccttgtc
     2761 cagcacgtgg ggcacggagt tgttggtgta gttggcgagc ctggccgacg gtccgtgagg
     2821 catgccgccc tgcaggacga gctggctggt ggcacaggcg ccgccctcgt gaatgacgtc
     2881 cgcgtagcgg ccggcgcgct ccttgaagtg gggcatgcgg aagacgccgc gggtgatggc
     2941 gccgaggccc gtgggcagaa acccgggaac catcagcgtg ttgtccacga aacccgtgat
     3001 cccgtcgatc caggcgaccc cgtcgcgggc ccgtcgggcc cagtaggcca ggttctgctc
     3061 ggcggcttcc tcgctgcccc agaggtcgcc gatgaacccg ccgtgcggtg gcatcatgat
     3121 gcggttgcgc aggttcatgg tgccgatctg aatcggctga aacaggtgtg tgagctcgct
     3181 catggtctcc ccttcgaccg tatcccccag cggctcgtta ttcaccaatt gcggctcgat
     3241 tacaaccccg gcgcgtggca cgatggatcg cggaggtagc ctcatgtccg aagagcccga
     3301 agacgttcag atccatctcg acagcgtgga tcacgtgctg agcacggcgc gctcggtgcg
     3361 gcgcaagctc gacttttccc gtccggtgga cccggaggtg atcttcgact gcatcaacgt
     3421 ctccacccag gcgcccaccg gccgtggcgg cgaaagctgg cgcttcctgg tggtgacgga
     3481 cttgggccgg aagcgggaaa tcgccggcct ttaccgggac gtgctggtgg ggcttcagca
     3541 ggagcggggg ctcgtcatca agcccaccca gcaggccctg gtggatcggc tgcacgagat
     3601 tccggccatg atcttcgtct gctccatggg cgcgccgccg gggccggaaa tacctaccca
     3661 ggtggcctac tacggctcgg tgctgccctc ggcgtggtcg ctgatgctgt ctcttcgcgc
     3721 ccgcaacctg ggcgccacct ggaccacgct gctctcctcg cagcaggaag cgctcggccg
     3781 catcctcgac gttcccgaag acgtggtctt caccgtcatg ctgccggtgg gctacatgaa
     3841 gggggctcgc ctcaagaagg ccgaccggat ggacgcccga cgggttacct tctggaatcg
     3901 ctggggcaac gacgcgccgc attgaagcgc tgttgctgga tcagtagagc ggctccacgt
     3961 cgaaggccac gcagacgcgc tcctgatcgc tccggaacgg aacggtcccg tgatagaagt
     4021 aggacgggaa cagaaacatc tggccgtagg ccggctggta gtggcgcagg gtcagctcgg
     4081 actgcacgtg cagatcggtc gtagggcggc cgaactccag ccagccttcg ggctttcggg
     4141 ctgggtcggc aatcagatcc ggcagctcga ggtagaacac gccgctcagc cagccgttgg
     4201 ggtgaatgtg ggcgcgctcg tggccgcggt cgaacatcac gacgccccag gtggtcagct
     4261 tccaggcttt cggcacccag cgcacggcgg gatgctgcgg atcgtccggc agctggc
//
LOCUS       DBNHQR010000082         2745 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_261330, whole genome shotgun sequence.
ACCESSION   DBNHQR010000082 DBNHQR010000000
VERSION     DBNHQR010000082.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_261330"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="AC16VR_01830"
     CDS             <1..885
                     /locus_tag="AC16VR_01830"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR6009920.1"
                     /translation="SGGAHALRVPRWMGALALAIGLVATAAGAVLGMNLLGSHQMAPE
                     TVVHWRQEVEAQRLELQALLDRSTAETQAVGRQLAQMQARLLRMEALGARVAEVADLD
                     DGEFSFDLPVPVGGPDTTTSEPMPMGDLRADVDALARNLKIRETELEILESMLRDRDY
                     RAEVAVAGRPVTWGWISSSYGERVDPFSGKMAWHAGLDFAGKQGSDVIAVASGVVTHA
                     GNRFGYGEMVEVTHGDGYVTRYGHHAEIVVAVGEIVKKGQVVGKMGSSGRSTGPHVHF
                     EVLKNGRRVDPARYVVRR"
     gene            1068..>2745
                     /gene="secA"
                     /locus_tag="AC16VR_01835"
     CDS             1068..>2745
                     /gene="secA"
                     /locus_tag="AC16VR_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR6009921.1"
                     /translation="MLKKIFGTKNARELKRMGRIVARINALEADLLEISDDELKATTG
                     RLKERLEQGASLEDLLPEAFAAVRESARRAKDMRHFDVQLIGGITLHEGRIAEMRTGE
                     GKTLVATLAAYLNALAGRGVHVVTVNDYLARRDAEWMGPIYETLGLTVGVIQSGQAPQ
                     EKREAYQSDVTYGTNNEFGFDYLRDNMAFSLADKFQRDLGYAIVDEVDSILIDEARTP
                     LIISGPAEQSTELYQKIDRIAPKLKQQPPVEDPEAEPTGDFVVDEKNRQVELTEVGHE
                     KVEAELMRLGLLEEGDSLYAAANLNLLHHVHAALKAHALFKRDVDYMVANGEVVIVDE
                     HTGRAMPGRRWSEGIHQAIEAKERVAIQNETQTLASTTFQNYFRLYDKLAGMTGTADT
                     EAFEFRQIYGLDVVVIPTHMPMVREDNNDLVYLTIEEKYDAIVEDINDCIERGQPVLV
                     GTASIESSERLSGELDRRKIAHNVLNAKQHEREADIIAQAGSPGAVTIATNMAGRGTD
                     IVLGGNWEAEAAQLDSPTDEELQRLRGEWQKRHDAVIAAGGLHIIGTERHE"
BASE COUNT          517 a          863 c          922 g          443 t
ORIGIN      
        1 tcgggtggtg ctcatgcgct gcgggtgccc cgctggatgg gggcgctggc gcttgccatc
       61 ggcctcgtag cgaccgctgc gggcgccgtg cttgggatga acctcctcgg gagccatcag
      121 atggccccgg aaaccgtcgt ccattggcgc caggaggtcg aggctcagcg cctcgagctg
      181 caggccctgc tcgatcgatc caccgcggaa acccaggccg tcggccgaca gcttgcccag
      241 atgcaggcgc ggcttctgcg catggaggcg ctcggagccc gcgtggcgga agtggccgac
      301 ctggacgacg gcgagttctc gttcgatctg cccgttcccg ttggtggccc ggacacgacc
      361 acttcggagc cgatgccgat gggggatctg cgcgccgacg tggacgctct ggcaaggaat
      421 ctgaagattc gcgaaacgga gctggagatt cttgaatcca tgctgcgcga ccgcgactac
      481 cgggctgagg tggccgtggc cggccgcccg gtgacgtggg gctggatctc ctcctcgtac
      541 ggtgagcggg tggacccgtt cagcggcaag atggcctggc acgcgggatt ggacttcgcc
      601 ggcaaacagg gttctgacgt catcgcggtg gccagcggcg tggttaccca tgcgggcaat
      661 cgcttcgggt acggagagat ggtcgaggtc acccacggcg acgggtacgt cacccgatac
      721 ggccaccacg cggaaatcgt tgttgccgtc ggcgagatcg tcaagaaagg ccaggtcgtt
      781 ggaaagatgg gctccagcgg ccgttccact ggcccgcacg tgcatttcga ggttttgaag
      841 aacggccgtc gagtcgatcc agcccgttac gtcgtacgtc gctgacgcga tccggcagcc
      901 ccgatctggt ctcgacacgc gccggcaggc ccggaaccca gccgaccttc gctgccttgt
      961 cgggcaggct ccatccttca tcggatgaag cgatcgtctc ccgcggattg tggtcgaatt
     1021 agggtaaact gccgccggtt tcgcatccgg cacccaggcg aaagcacatg ctcaagaaaa
     1081 ttttcggcac caagaacgcg cgagagctca agcggatggg caggatcgtg gcccggatca
     1141 acgcgctcga ggccgacctg ctcgagattt ccgacgacga actcaaggcc accaccggcc
     1201 ggctgaagga acggctggag cagggcgcca gtctggagga cctgctcccg gaagcgtttg
     1261 ccgcggtgcg tgagtctgcc cgccgcgcca aggacatgcg ccatttcgac gtccagctga
     1321 ttggtgggat caccctccac gaggggcgca tcgcggagat gcgcaccggc gagggcaaga
     1381 ccctggtggc gacgctggcc gcctacctca acgccctggc cggtcgcggg gtgcacgtgg
     1441 tgaccgtcaa cgactacctt gcacgacgcg acgcggagtg gatgggaccg atctacgaga
     1501 cgctcggcct cacggtgggc gtgatccagt cgggtcaggc gccgcaagag aagcgggaag
     1561 cctatcagtc ggacgtgacc tacggcacga acaacgagtt cggcttcgat tacctgcgcg
     1621 acaacatggc gttctcgctg gccgataagt ttcagcgtga cctcggttac gccattgtcg
     1681 acgaggtgga ctccattctc atcgacgagg cccgcacgcc gctcatcatc tccggccccg
     1741 ccgagcagag cacggagctt tatcagaaga tcgaccggat cgcgcccaag ctcaagcagc
     1801 aaccgccggt cgaggatccg gaagccgagc cgacgggcga tttcgtggtg gacgagaaaa
     1861 accgccaggt ggagctgacc gaggtcggcc atgagaaggt ggaggcggag ctcatgcggc
     1921 tcggcctcct cgaggaaggc gacagccttt acgcggccgc aaacctgaac ctgctgcatc
     1981 acgtgcatgc ggcactcaag gcacacgccc tgttcaagcg ggacgtggat tacatggtgg
     2041 ccaacggtga ggtggtcatc gtcgatgagc atactggtcg ggccatgccg ggcaggcgct
     2101 ggtccgaggg cattcatcag gccatcgagg ccaaggaacg ggtggcgatt cagaacgaga
     2161 cccagaccct ggcctcgacc acgttccaga actacttccg gctctacgac aagcttgccg
     2221 gcatgacggg caccgccgac accgaggcgt tcgagttccg gcagatctac ggcctggacg
     2281 tcgtcgtgat ccccacccac atgcccatgg ttcgggaaga caacaacgat ctggtttacc
     2341 tcaccatcga ggaaaagtac gacgcgatcg tcgaggacat caacgactgc atcgagcgcg
     2401 gccagccggt gctggtgggg accgcctcca tcgaatcttc cgagcggctg tctggggagc
     2461 ttgatcgccg gaagatcgct cacaacgtgc tcaacgccaa gcagcacgag cgcgaggccg
     2521 acatcattgc ccaggccggc agcccgggcg cggtgaccat cgccacgaac atggccggtc
     2581 gaggcacgga catcgtgctc ggcggcaact gggaagccga ggcggcccag ctggattcgc
     2641 ctaccgatga ggagctgcag cgcctgcgcg gcgaatggca gaagcgccac gatgcggtca
     2701 ttgctgccgg tggcctgcac atcatcggca ccgagcgcca cgagt
//
LOCUS       DBNHQR010000083         2409 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1147620, whole genome shotgun sequence.
ACCESSION   DBNHQR010000083 DBNHQR010000000
VERSION     DBNHQR010000083.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1147620"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="AC16VR_01840"
     CDS             complement(<1..632)
                     /locus_tag="AC16VR_01840"
                     /inference="COORDINATES: protein motif:HMM:NF016764.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0016192 - vesicle-mediated transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HIR6009922.1"
                     /translation="MSGVHEPDTAVYTRRREELIDEGSGSLAVTTSIPASMMLRLLIR
                     PRSVFRLLADADPAPLVVFFKFALWLGLLPPLFAYIGASTFGWRVGVVEPLFLPQPTL
                     IAISIAYFCSLLFGFFSASIISRWMAVTYGARDSLGVHLAMMVIVGAPLAIASLMHLY
                     PNALLNAVVFVPTMIWSMYLLYRGLPTILHTTPEQGMLMASSIIGYLLVAA"
     gene            complement(678..2018)
                     /locus_tag="AC16VR_01845"
     CDS             complement(678..2018)
                     /locus_tag="AC16VR_01845"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014503.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase/lipase family protein"
                     /protein_id="HIR6009923.1"
                     /translation="MTAAAAGCAQFGVPPLKRLYQSVPVPEDQPPLIVIPGAFGSKLR
                     RRATGEEIWPRSGFALLFSSYPDVEVTIDADTLDPIADEVEAFDIFRKGMGRDFYGAL
                     LKTLESIGGYQRAHPGTPPAPPARSYYVYPYDWRLDNVAAVEGLHRLIEQVVEDHGDP
                     NLRVDILGHSNGGLMARYYARFGTADMLTSRAAEPDWNGSHRIRRLLMVGTPNLGTMQ
                     PVLSHVRGEEIGLNDIPAEVVATCSGAPQLFPAPELTWLVDARGEDLNLDVYALETWR
                     ELGWSIFSDKARDRATSKHGVGRRGRAHLELLERYLAKHLERGRRFQHALARPARPDE
                     PEPFVFGGDCTPTVARLVLEKRGGVFVGREQPDRVAAPVRGVDYDGLINEPGDGVVTR
                     ASLTGQNATPVAPQLNVAHSVFLCEEHQLLTSNASFQDNLLYNLLTEFPQGAHG"
     gene            complement(2060..>2409)
                     /locus_tag="AC16VR_01850"
     CDS             complement(2060..>2409)
                     /locus_tag="AC16VR_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009924.1"
                     /translation="QHPLIMLDWQGPLRGRGIYDVAQLLVQSAQTEVRRSHERQLVER
                     YVQKLTDQGVTGYGFDQAWEDYRVATLYLWAYVVVVSGTLEVGSERAFAWMSKMVQRN
                     ASAIEDLDCLSLL"
BASE COUNT          437 a          773 c          807 g          392 t
ORIGIN      
        1 gcggcaacca gcaggtagcc gatgatcgat gaggccatga gcatcccctg ctcgggggtg
       61 gtgtgcagga tcgtcggcag tcctctgtag agcaggtaca tgctccagat catcgtcggg
      121 acgaacacga cggcgttcag cagggcgttg ggatacagat gcatcaggct ggcgatggcc
      181 agcggtgcgc cgacgatgac catcatggcc agatgcacgc ccaggctgtc ccgcgcgccg
      241 taggtcaccg ccatccagcg actgatgatc gacgcgctga agaagccgaa gagcagcgag
      301 cagaaatagg cgatgctgat cgcgatcagc gtcggctgcg gcagaaacag cggctccacc
      361 acgccgaccc gccagccgaa cgtggacgcg ccgatgtaag cgaagagtgg cggaagcagg
      421 ccgagccaga gtgcgaactt gaagaacacg accagcggcg cgggatccgc atctgcgagc
      481 aggcggaaaa ccgatctggg tcggatcagc aggcgcagca tcatggaggc cggaatggag
      541 gttgtgacgg ccagtgaccc gctcccctcg tcgatcaact cttcgcgacg gcgagtatag
      601 acggccgtgt cgggttcatg aactccggac aatccacgat tgccgggatg cggccaggac
      661 gtggcgacgt cccggaatca cccgtgtgcg ccctggggga actcggtgag gagattgtag
      721 agcaggttgt cctgaaagct tgcgttgctg gtgagaagct ggtgctcctc gcacagaaac
      781 accgagtgcg cgacgttcag ctggggggcg acgggcgttg cgttctggcc cgtcagcgag
      841 gcgcgcgtca ccacgccgtc gccgggttcg ttgatgagac cgtcgtagtc cacaccgcgc
      901 acgggagccg ccacccggtc cggttgctcg cgtccgacga atacgccgcc gcgcttttcc
      961 agcaccagcc gcgccaccgt tggcgtgcag tcgccgccga agacgaatgg ttccggctcg
     1021 tcgggccgtg ccggtcgtgc cagagcgtgc tggaatcgcc ttccgcgctc gaggtgtttg
     1081 gccaggtagc gctcgagcag ctcgagatgt gcccggcccc ggcggcccac gccgtgcttg
     1141 ctcgtcgccc gatctctggc tttgtcgctg aaaatggacc acccaagctc gcgccaggtc
     1201 tccagcgcat agacgtccag gttcaggtct tcgccgcggg catccaccag ccaggtcagc
     1261 tccggagcgg gaaagagctg cggggcgccg gagcaggtgg cgaccacctc tgcgggaatg
     1321 tcattgaggc cgatctcctc gcccctgacg tgggacaaca ctggctgcat ggtgccaagg
     1381 ttcggggtgc ccaccatcag cagccgccga atcctgtggc ttccgttcca gtcgggctcg
     1441 gccgcgcgac tggtgagcat gtctgccgtg ccgaagcgag cgtagtagcg agccatcaga
     1501 ccgccgttcg agtggccgag gatgtcgacg cgaagattcg ggtcgccgtg gtcctcgacc
     1561 acctgctcga tcagccggtg gagtccctct acagcagcaa cgttgtccag ccgccagtcg
     1621 taggggtaca cgtagtaaga ccgcgcgggc ggcgccggtg gcgtacccgg atgcgcccgc
     1681 tggtaaccgc cgatcgattc caacgttttc agcagcgcgc cgtagaaatc cctgcccatc
     1741 cccttgcgga agatgtcgaa ggcctctacc tcgtcggcga tcggatccag ggtgtccgca
     1801 tcgatggtga cctccacgtc ggggtagctg ctgaacagca gcgcgaagcc cgagcgcggc
     1861 cagatctcct cgccggtggc ccgccggcgc agcttggagc cgaaggcacc gggaatcacg
     1921 atgagcggcg gctgatcctc gggcaccggc accgattgat agagccgctt cagcggaggc
     1981 acgccgaact gcgcgcagcc ggcggccgcg gcggtcatcg ccgccgacag aaaggcgcgg
     2041 cgcggccaac catgtggaac tacaacaggc tcaggcaatc cagatcctcg atggccgatg
     2101 cgttgcgctg aaccatcttc gacatccagg cgaaggcgcg ttcgctgcca acctccaggg
     2161 tgccgctgac gacgaccacg tatgcccaca ggtaaagggt tgctacccgg tagtcttccc
     2221 aggcctgatc gaagccgtag ccggtcacgc cttggtcggt cagtttctgc acgtagcgtt
     2281 ccaccagctg tcgttcgtgg gaacggcgca cctcggtctg agcgctctgc acaagcagct
     2341 gcgcgacgtc gtagatgccg cgccctcgca gcggaccctg ccagtccagc atgatcagcg
     2401 ggtgctgct
//
LOCUS       DBNHQR010000084         4684 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_387833, whole genome shotgun sequence.
ACCESSION   DBNHQR010000084 DBNHQR010000000
VERSION     DBNHQR010000084.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4684)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4684
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_387833"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="AC16VR_01855"
     CDS             <1..194
                     /locus_tag="AC16VR_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6009925.1"
                     /translation="GENGRSTQARYPNIAGQNAPYLEARLKYFRDGVEHNNRMNGQAA
                     PLSDEDIAGLASYFSRMPR"
     gene            231..521
                     /locus_tag="AC16VR_01860"
     CDS             231..521
                     /locus_tag="AC16VR_01860"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6009926.1"
                     /translation="MASILVLSLSAGQTAAAEAGEGAALASEKGCVACHGKDGVAIAP
                     IYPNLAGQWKRYLREQLHAYRSGRRQNNVMQGFAAGLSDDDIRRLADHYGSR"
     gene            525..1739
                     /locus_tag="AC16VR_01865"
     CDS             525..1739
                     /locus_tag="AC16VR_01865"
                     /inference="COORDINATES: protein motif:HMM:NF015406.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HIR6009927.1"
                     /translation="MSWPANRSAGLTRLEDWVPRAGRAYQARRNYDCGPGDRQNVSGL
                     SPYIRHRLVDEREVLEAVLAAHAHSAADKFVQEVFWRTYWKGWLEHHPQAWARYRTEL
                     EAVLETLSLDASTSERYGRAVAGETGMEPFDGWVEELTANGYLHNHARMWFASIWVHT
                     LGLPWVLGADFFMRHLLDGDPASNTLSWRWVVGLHTAGKTYLARPDNITRYTEGRLQN
                     ISGLVGAAHALQEAELARETLRLPGFCLPSGARLGLLLTDEDLSLPELPTNPVAIKGV
                     SLTRMRSPLGVSGAVRAFADDAIQDALGRASERYGCAAVGEIGAEDQEGVLAWVREQS
                     LDGVLVNYPTVGPARQFLEALAAPLGDAHVGSFPLVSEYDRLCWPHADRGYFKLRKRI
                     PELLGALDIAAG"
     gene            1914..2156
                     /locus_tag="AC16VR_01870"
     CDS             1914..2156
                     /locus_tag="AC16VR_01870"
                     /inference="COORDINATES: protein motif:HMM:NF024845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HIR6009928.1"
                     /translation="MCEACVEEMALKVQAAEETARSCPVDGAKMTKEIAHMIVIDRCP
                     TCQGVWLDGGELDRIRGGVEQEALMAMTGRFTYPLA"
     gene            complement(2195..3352)
                     /locus_tag="AC16VR_01875"
     CDS             complement(2195..3352)
                     /locus_tag="AC16VR_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009929.1"
                     /translation="MAISAVVDFLEATSSDDALKAELVKTLGVGDGDVSDAAELDENE
                     SAALLGQAGVDACALAAGKGFDFSVNELQAVVGAFQSFRSGEITDEELMSMLGLPSVP
                     SPSAQEAVMLSYRGVTHARATSGAGEGKRRLDVVRFVEATAKDDRLRSELQSILQAGD
                     GDISDFSELDEGELQALRSARGALVADFAAKHGYTFTIADLYSIIDAFQRMKGGELSD
                     TAFEKFIDASSQSADYLPFIDSIATMTYKGFTYDRVKPAVAQGNSLEVVRFLQKSHDD
                     ADLRGKLQEIIGGDGDVSSPEELDSSEAGNLIGARSARVVALATSEGFRFTISDLSAV
                     IGAFQLVEQGTLSLESCKRILGFADSAASEAFGNAGTTAVRIYRGVPVAAK"
     gene            3749..>4684
                     /locus_tag="AC16VR_01880"
     CDS             3749..>4684
                     /locus_tag="AC16VR_01880"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR6009930.1"
                     /translation="MNSASLKFPGLLLCLILAATALPVPVFAAEEGFAIEELVVTARK
                     REENIQDVPVSITAFDATAIEDMYGNTIGEFTKYAPNVTLARQPYAGNALFGGMRGIV
                     FGDLEKSFDPAVGVVVDGIALVTNTSALIDTFDLEYAEILRGPQGTLFGRNTIAGVVN
                     LRRSRPTGELGAKFQFRYGSHNETDLKGVFNFPVTDTIAVKVAAFMDKGDGFQEEADF
                     NLQTGEITGTGDDIDGEDTFNTYASVLWTPTDSFDALLTFEYGNDDSTLSTPTNLTTP
                     NIEALWGPISQAMFAGLVAGDPNAVGDAIVAVAING"
BASE COUNT          847 a         1396 c         1546 g          895 t
ORIGIN      
        1 acggggagaa cggccggtcg acgcaggccc ggtacccgaa cattgccggg cagaacgcgc
       61 cgtatctgga agcccggttg aagtactttc gcgatggcgt cgagcacaac aatcgcatga
      121 acggtcaggc ggctccgctc tccgacgagg acatcgcggg tcttgccagt tatttcagcc
      181 gcatgccgcg gtgacgggaa atggaagtga gaagtttgaa caggatattc gtggcgtcga
      241 ttttggtttt aagtctttcc gccgggcaaa cggccgccgc agaagcaggt gagggagcgg
      301 cgctcgcctc ggagaagggg tgcgttgcct gtcatgggaa agacggcgta gccatcgcgc
      361 ccatctatcc caacctggcc ggacagtgga aacgttacct tcgcgagcag ctgcacgcct
      421 accggtccgg caggcgtcag aacaacgtga tgcagggttt tgccgccggg cttagcgacg
      481 atgacatccg gaggctggcc gatcactatg gttccaggta gacgatgagc tggccggcca
      541 accggtctgc cgggctgacc aggctcgaag actgggttcc gcgagccggc cgggcctatc
      601 aggcgcgtcg caactacgac tgtggccccg gggatcgcca gaacgtctcg ggcctgtcgc
      661 cttacatcag acaccgactc gtcgatgagc gcgaggtgct cgaggccgtg ctcgccgcgc
      721 acgcccactc ggcggcggac aagttcgttc aggaggtgtt ctggcgaact tactggaaag
      781 gctggctgga gcatcatccc caggcctggg cgcgctaccg caccgagctg gaagccgttc
      841 tggagacgct ttcgttggat gcgtcgacgt cggagcgcta cgggcgagcc gtggcggggg
      901 aaacgggcat ggagccgttc gatggctggg tcgaagagct cacggcgaac ggttacctgc
      961 acaaccacgc ccgcatgtgg ttcgccagca tctgggtcca cacgcttgga ctgccctggg
     1021 tgctgggtgc ggattttttc atgcgtcacc tccttgacgg ggatccggcg tcgaacacct
     1081 tgtcctggcg ctgggtcgta gggttgcaca cggccggaaa aacctacctt gcgcggccgg
     1141 acaacattac tcgttacact gaagggcgcc ttcagaacat ctccggcctc gtcggggccg
     1201 cgcacgccct gcaggaggcc gagcttgcgc gcgagacgct gcgcctgccc gggttctgcc
     1261 tgccgtcggg cgctcgcctt gggcttctgc tcacggatga ggatctatcg ctgcctgagc
     1321 tgcccacgaa ccctgtggcg atcaaaggtg tgagcctgac gcgcatgcgg tctccgcttg
     1381 gcgtatctgg ggcggttcgg gcgtttgccg atgatgcgat ccaggacgcc ctgggccggg
     1441 cgagtgagcg gtacggatgc gcggccgttg gggagattgg agctgaggac caggagggcg
     1501 tcctcgcctg ggtgcgggag cagtctctgg acggggtcct ggtcaattac ccgacggtcg
     1561 gacctgcgcg tcagttcctg gaggctctgg ccgcgcccct gggagatgcg cacgtgggat
     1621 cgttcccgct cgtttcagaa tacgatcggc tgtgctggcc tcacgcggat cgcggctatt
     1681 tcaagctgcg caaacggatt cccgagctcc ttggcgctct cgacattgcc gccggctgaa
     1741 atcgtccgtg gacgagaccg gcaggggctc ggcaggggca ggagttcatt gtggtgggcg
     1801 cagatggggc attatcatag gggcctcgga ggaggagaaa tgctatgtcg ctgttcggcg
     1861 agaagtgcga tcggtgcggt acgagaacga agaataagca ggacggccgc cccatgtgtg
     1921 aagcctgcgt ggaagagatg gcgctgaagg ttcaggctgc tgaggagaca gcccggtcgt
     1981 gtcctgtgga tggcgcgaag atgaccaaag agattgctca catgatcgtg attgatcgct
     2041 gccccacctg ccagggagtc tggctcgacg gcggcgagct cgaccgaatt cgcggcgggg
     2101 tcgagcagga agcgctgatg gccatgacgg ggcggttcac ctacccgcta gcctagaggc
     2161 gccggcgtgc aggtttgagg accggtcggt agcctcactt ggcggcaacc ggaacgccgc
     2221 gataaatgcg aacggccgtg gtccccgcgt tgccgaatgc ttcgctcgct gcggagtccg
     2281 cgaaccctag aatgcgcttg cagctctcca gtgacagtgt gccttgctcg acgagctgaa
     2341 aagcgccgat cacggcgctc agatcggaga tcgtgaagcg aaatccttcg gaggtggcta
     2401 gtgccaccac ccgggcgctg cgcgcgccaa tcaggtttcc cgcctcggaa ctgtcgagtt
     2461 cctcaggtga gctgacgtcg ccgtcgccgc cgatgatttc ctgaagcttg ccacgaaggt
     2521 ctgcgtcatc gtgggacttc tgcaggaagc gcacgacctc cagggagttg ccctgggcga
     2581 cggcgggctt cacccggtcg taggtgaacc ctttgtaggt catggtcgcg atggagtcga
     2641 tgaagggcag atagtcggcg gactggctgc tggcatcgat gaacttttca aaagcggtgt
     2701 cggaaagctc gccgcctttc atccgctgga aggcgtcgat gatcgagtac agatccgcga
     2761 tggtgaacgt gtagccgtgc tttgcggcga agtccgcgac cagggcgccc ctggcgctgc
     2821 gcagtgcctg cagctcgcct tcgtcgagct cggagaagtc gctgatgtca ccgtcgccag
     2881 cctgcagaat ggactggagt tctgaacgca gtcggtcgtc cttggcggtg gcctccacga
     2941 agcggacgac atcgagcctt cgtttgcctt cgccggctcc ggatgtcgct ctggcgtggg
     3001 tcaccccccg gtacgagagc atcacggcct cctgagcgct tggcgaaggg acgctcggca
     3061 ggccaagcat gctcatgagc tcttcgtcgg taatctcgcc ggaccggaag ctctggaaag
     3121 cgcccaccac cgcctgaagc tcgttgacgg agaaatcgaa gcctttgccg gccgccagag
     3181 cgcaggcatc cacgccggcc tggccgagca atgctgcgga ttcgttctcg tcgagctccg
     3241 ccgcgtcgct cacgtcaccg tcgcccacgc cgagcgtctt tacgagttct gctttcaggg
     3301 catcgtcgga actcgttgct tccaagaaat ccaccacggc actgatcgcc atttcctctc
     3361 cttctgcgag ctgaacgcaa cgtcgttatt ggagttatag caactttcgg gccagaaacc
     3421 accgctgcgt aatgcgtgag atctggcgat cttcccggcc tgggcgcacc aaaccagggc
     3481 gggcggtcac ttgcggcgct ccggaagcgt gcggcgggca taagaattgt ctggcaaccc
     3541 ccgcgcggat ccattgttgc gcggcagcgg ctccagacca cctcgacccg cgggtacccg
     3601 ccagctcgct caattgcgtg tcagcaccgg tcagccagtg gagaaagttt gcggtgacgc
     3661 ttgggccctg gcggaattct tcgcgttgat tgctttttag gatcgtccca tactggggcc
     3721 cgggtgtatt gctgatatgg agagaataat gaactcagcg tctcttaagt ttccgggctt
     3781 gctgctgtgc ctgattttgg ctgcaactgc gctgcccgtt ccggtctttg cggccgagga
     3841 agggttcgcc atcgaggagc tggtggttac cgccagaaag cgagaggaga atattcagga
     3901 cgtgcccgtt tcgatcacgg cctttgacgc gaccgccatc gaggacatgt acggcaacac
     3961 tatcggcgag ttcaccaagt acgcgccgaa cgtcaccctg gcgcggcagc catatgcagg
     4021 caatgcgctc ttcggcggca tgcgggggat cgttttcggc gatctggaga agagcttcga
     4081 tcccgccgtt ggcgtggtgg tggatggcat agccctcgtc acgaacacca gcgctctgat
     4141 cgacaccttc gatctcgagt acgcagagat tctgcgcggc ccgcagggca cgctgttcgg
     4201 acgcaacacc attgccggtg ttgtcaacct cagacgctcc cgtcccacgg gcgagctcgg
     4261 cgccaaattt cagttccgct acggcagtca caacgaaacg gacctgaagg gagtgttcaa
     4321 cttccccgtc accgacacca tcgccgtgaa ggtcgccgcc ttcatggaca agggcgacgg
     4381 cttccaggaa gaggccgact tcaatttgca gaccggcgag atcacgggca ccggcgacga
     4441 catcgacggc gaggatacgt tcaacaccta cgcaagcgtt ctgtggacgc ccacggacag
     4501 cttcgatgcc ctgcttacct tcgagtacgg caacgacgat tcgactctga gcacacccac
     4561 caacctgacg acgcccaaca tcgaggcttt gtgggggccg atctcacaag caatgtttgc
     4621 agggctggtc gccggcgacc cgaatgcggt gggggacgcg atagttgcag tcgcgattaa
     4681 tggg
//
LOCUS       DBNHQR010000085         5636 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_388021, whole genome shotgun sequence.
ACCESSION   DBNHQR010000085 DBNHQR010000000
VERSION     DBNHQR010000085.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5636)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5636)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5636
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_388021"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(387..587)
                     /locus_tag="AC16VR_01885"
     CDS             complement(387..587)
                     /locus_tag="AC16VR_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009931.1"
                     /translation="MKNFRIRPLHRQRNGRRQRVRVLDQNELADLLGVPITELRKELD
                     DRGWTYHEDSTGNLWATRPERP"
     gene            complement(649..2037)
                     /locus_tag="AC16VR_01890"
     CDS             complement(649..2037)
                     /locus_tag="AC16VR_01890"
                     /inference="COORDINATES: protein motif:HMM:NF023222.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6009932.1"
                     /translation="MTGDEYRASLNDGRETFFEGKQVDDLPNHPILGITVESAASGYD
                     RFYDPAPDAVGAFMKVPGSAVELREQVELHETVDLLTHVTYASIMTLLTAADRIEAQL
                     PDNARRIREYVRDVQRKDLRITQCITDAKGDRSRPPGKQDDPDAYVRVIERRADGVVI
                     RGAKLHISAASMGHELMTIPTKSMKPGEDDYAVACMVPVNAPGVKIVNTTYAPRHEDK
                     RDFPISGSHHTPEGFVIFDDVFVPDERVFLDGPPEFAAVFAHSLGLWERLGGLASMAS
                     SYDRLVGFAQLICEANGLERTGHIKEKISEMMINATLIRASLEAAIDNCSITSDGAAF
                     PNELYTNVGKYHGAANWSAMVRHLHDIAGGSILTAPGMADLENEKTGHLIRKYMGTTQ
                     EVDGVYRTRLFHAIRDMTADALGGWNAVTNIQAGGGLYAQRIVSRMHYDIDKAKRMAL
                     ESAHMQEYLSDA"
     gene            complement(2085..2771)
                     /locus_tag="AC16VR_01895"
     CDS             complement(2085..2771)
                     /locus_tag="AC16VR_01895"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6009933.1"
                     /translation="MTGRRLTGKRVLITQADDYMGPATAELFASHGAQIIADARDLRP
                     AGACEALVHEAGRVDVLIANLAGPNFSGTPATAIDDEAWATVFDTMVHPLHRLTRAVL
                     PQMQDRQSGKIIVYGSATALRGLKTVAAYSAARAAQVGYVQAVGVEVAPLNVQVNLIA
                     QNYVENPVYYPPELTAKESFQRSLKRQVPLGRLATAREDAQFALFLASDESDFFVGQA
                     IPFSGGWVQR"
     gene            2963..3805
                     /locus_tag="AC16VR_01900"
     CDS             2963..3805
                     /locus_tag="AC16VR_01900"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6009934.1"
                     /translation="MSQGCVYGLIALGFVLIYKATEMVNFAQGDVMMLGAFLAFALIN
                     LMELPFLWGFMGALAAMAVVGVLLERLLLRPMIGEPHFAVLMLTIGLGFILRAVAGAI
                     WGNEPRALHTPYGGEVLRFGDLVVGYENIVIIVGTAVLCGLLFAFFRFTRVGVAMQAA
                     SQNQLAAYYVGIPVKRIYSLVWALSATIAACAGVLIAPVTLIDPLMGSFLGIKAFAAA
                     IGGGFGSLPGAILGGLLVGITEQFVGLYFPPGFADVSAYVLLLVMLMIRPEGLFATMQ
                     RKKV"
     gene            3810..4886
                     /locus_tag="AC16VR_01905"
     CDS             3810..4886
                     /locus_tag="AC16VR_01905"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6009935.1"
                     /translation="MRFIRKTHYVQDVDLIKHPGQRFWYGLLAIVLVLAPLGLDRFYL
                     GELSLVFIYAIAGIGLMLLVGYTGLVSLGHAAFLAVGAYCHSWLLGNGVPLPLAMVAA
                     TLVTAGVGAVVGVPALRMTGIYLAVATLALAVIVEKVLEKWETVTGGLRGLPVPKAQF
                     LGFDLGAPANFYYLCLGVLALGLLAAMNLLRSPTGRAMVAVRDSETSAQSMGIHINRI
                     KGLAFALSAGFTGLAGALFAHRIGYLFPDSFTVLTSIQLLLMVVVGGLGSLHGVVFGA
                     IFIGLLPQGIALFRDVVPPAIAQIPGLEPGIFGLILVLFLIFEPLGIYGRWVKIRLFF
                     EEFPLYRRATYKRQKTYLRTERLR"
     gene            4883..>5636
                     /locus_tag="AC16VR_01910"
     CDS             4883..>5636
                     /locus_tag="AC16VR_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6009936.1"
                     /translation="MSLFAAEDVAVSFGGIKAVHDLSFAVEDGEVFSIVGPNGAGKTT
                     IFNLISRIYDVDQGSLAFDGTEITRLPAHAVAELGVARTFQNTELFEQETVLKNLLIG
                     CHVHRRSGLLADILFLPSVKAQELTFRKRVEDVIDLLDLQSYRDQVIGNLPYGVRKMV
                     EIARALCLGPKLLLLDEPSSGLNPEETEDLSFWLEDINNDLGITIIMVEHDMNLVSRA
                     SDRVMALADGALLTIGSPADVQQHPDVLRAYLG"
BASE COUNT          848 a         1777 c         1871 g         1140 t
ORIGIN      
        1 agccggggcg cgtgcatcat accgcccggt ttcttcaggg caaaaaaaag ccccgccgaa
       61 gcggggctag ccatttggtc atcacaaggt ctatggagag tgtgttgctc ttcagctaga
      121 gggttacgaa tgcagccagg ctcagcatca gggtgagcag agcgccgagg agtagtgcgc
      181 tctcacctag ggtcagctgc ccctggtccc tgtgtgcggc tatcgctgcc gcactggaca
      241 tttgcgtatg gggttcgtta cgcagatctc gcatccgctc cggcccgttc aaaacaacac
      301 gatagaggtt aggcggtaac tctgcagcca ggacataagg attctggaat gaatatggag
      361 ggttcaaaag ggccgaaacg cccgtctcat gggcgttctg ggcgggtcgc ccacaggttg
      421 ccggtgctgt cctcatggta ggtccaaccc cgatcgtcca gttccttgcg caattcggta
      481 atcggcaccc cgagcaggtc ggccagctcg ttctggtcga gcacccgcac tctttgccgg
      541 cggccgttgc gctgacggtg cagcggccga atcctgaaat tcttcatccg ctgtgctgcg
      601 cggctcggtc ccaataactg tatatacata cagtatgcgg cgcaacgatc aggcgtcgct
      661 gagatactcc tgcatgtggg cggactccag cgccatccgc ttggccttgt cgatgtcgta
      721 gtgcatgcgc gacacgatcc gttgcgcgta gaggccgccg ccggcctgga tgttggtcac
      781 ggcgttccac ccgcccagcg cgtcggcggt catgtcgcga atggcgtgga agagccgggt
      841 gcggtaaacg ccgtccacct cctgcgtcgt gcccatgtac ttgcggatca gatgtcccgt
      901 cttctcgttc tccaggtcgg ccatgcccgg cgcggtgagg atgctgccac cggcgatgtc
      961 gtggagatga cggaccatgg cggaccagtt ggcggccccg tgatactttc ccacgttggt
     1021 gtaaagctcg ttcgggaacg ccgcgccgtc cgaggtgatg ctgcagttgt cgatggcggc
     1081 ctcgaggctc gcgcggatga gggtggcgtt gatcatcatc tcggagatct tctccttgat
     1141 gtgccccgtg cgctccagcc cgtttgcctc gcagatgagc tgcgcaaatc ccaccagccg
     1201 gtcgtagctc gacgccatag acgccaggcc gccgagccgt tcccacaggc ccagggagtg
     1261 ggcgaacacg gcggcgaact cgggggggcc gtccaggaac acccgctcgt cgggcacgaa
     1321 cacgtcgtcg aagatcacga agccctcggg cgtgtggtgt gatccggaga tcggaaagtc
     1381 gcgcttgtcc tcgtgtcgtg gggcataggt ggtgttcacg atcttaaccc ccggcgcgtt
     1441 gacgggaacc atgcatgcca cggcgtagtc gtcctcgccg ggcttcatgg acttggtcgg
     1501 gatggtcatc agctcgtggc ccatggaggc tgccgagatg tgcagcttgg ccccccggat
     1561 gaccacgccg tcggcccgtc gctcgatgac gcgcacgtag gcgtcggggt cgtcctgctt
     1621 gcccggtggc cggctgcggt cgcccttggc gtcggtgatg cactgggtga tgcgcaggtc
     1681 tttgcgctga acgtcgcgga cgtactcgcg gatgcgccgc gcgttatcgg gcagctgcgc
     1741 ttcgatccgg tccgccgcgg tcagcagcgt catgatggag gcgtaggtga cgtgggtcag
     1801 cagatccacg gtctcgtgga gctccacctg ctcgcgcagc tcgaccgcgg agccgggcac
     1861 cttcatgaag gcgccaaccg cgtccggggc gggatcgtag aagcggtcgt agccgctggc
     1921 cgctgactcc acggtgatcc cgaggatcgg gtggttgggc aggtcgtcca cctgcttacc
     1981 ctcgaagaac gtctcgcgcc cgtcgttcag tgaggcgcga tactcgtcgc cggtcatcat
     2041 ctggcaatcc ccctcaagcc caacgcgaag gatggtaaat gagcctagcg ttgaacccaa
     2101 ccgccgctga agggaatggc ctggccgacg aagaagtcgc tctcgtcgct ggccaggaac
     2161 agcgcgaact gcgcatcctc ccgggcggtg gccagcctgc cgagcggcac ctggcgcttg
     2221 agtgatcgct ggaacgactc cttggccgtc agctccggcg ggtagtagac ggggttctcc
     2281 acgtagttct gggcgatgag gttgacctgg acgttgagcg gcgcaacctc cacgcccacc
     2341 gcctgcacgt agcccacctg ggcggcgcgg gcggcgctgt aggcggcgac ggtcttcagc
     2401 ccccgcagcg ccgtggcgct cccgtagacg atgatcttgc cggattggcg gtcctgcatc
     2461 tgcggcagaa ccgcgcgggt caggcgatgc agggggtgaa ccatggtgtc gaagacggtt
     2521 gcccaggcct cgtcgtcgat ggccgtggcg ggcgtgccgc tgaagttggg cccggccagg
     2581 ttggcgatca gcacgtcgac ccggccggcc tcgtggacaa gggcctcgca cgcgcccgcc
     2641 ggtctgaggt cccgggcgtc ggcgatgatc tgcgcgccgt ggctggcgaa cagctcggcc
     2701 gtggctggcc ccatgtagtc gtcggcctgg gtgatgagca cccgtttgcc cgtgagtcgc
     2761 cgtccggtca ttttcctcgc tccgctgcct gatctccggg tgtgccgtcc attctgcccg
     2821 gagcgggtcc tggcgggaat ggcgctggca cggatccgag atgacaaagc agccgtcgat
     2881 tcggacaata ggcgtccgag gggatcaaaa accaaaacgc gccagccaag acgtggaagt
     2941 tctgcagttg ctaatcagcg gagtgtcgca ggggtgcgtc tatggattga tcgccctggg
     3001 gttcgtgctg atctacaagg ccacggagat ggtcaatttc gcccagggcg acgtcatgat
     3061 gctcggcgcg ttcctggcct tcgccctgat caacctcatg gagcttccct ttctgtgggg
     3121 cttcatgggc gcgctcgcgg ccatggccgt ggtgggggtg ctgctcgagc ggctgctgct
     3181 gcgtccgatg atcggcgagc cgcacttcgc cgtgctcatg ctcaccatcg ggctcggttt
     3241 catcctgcgc gccgtggccg gcgccatctg gggcaacgag cctcgtgccc tgcacacgcc
     3301 gtatggggga gaagtgctgc gcttcggtga cctggtggtc ggttacgaga acatcgtgat
     3361 catcgtcggc accgccgtgc tgtgcggcct gcttttcgcc ttcttccgct ttacccgggt
     3421 gggtgtcgcc atgcaggccg cttcccagaa tcagctcgcc gcttactacg tgggcattcc
     3481 ggtcaagcgc atctactccc ttgtctgggc tctgtcggcg accatcgccg cgtgcgcggg
     3541 cgtactcatc gcgcccgtca cgctgattga ccctctgatg gggagcttcc tgggcatcaa
     3601 agcgttcgcg gcggcgatcg gtggcggttt cggcagcctt cccggcgcca ttctaggcgg
     3661 tcttctggtg ggcatcacgg agcagttcgt cgggctctat tttccgccgg gattcgccga
     3721 tgtgagcgcc tacgtgctgc tgctcgtcat gctcatgatc cggcccgaag ggctcttcgc
     3781 gaccatgcag cgcaagaagg tctgaggccg tgcgtttcat ccgcaagacc cactacgttc
     3841 aggacgtcga cctgatcaag catccggggc agcgcttctg gtacgggctg ctcgccatcg
     3901 ttctcgtcct cgcgcccctg gggctcgacc ggttctacct gggcgagctg tctctcgttt
     3961 tcatctacgc catcgccggc atcgggctga tgctgctggt ggggtacacg ggcctcgtga
     4021 gcctgggcca tgcggccttt ctggccgtgg gcgcgtactg ccattcgtgg ctgctgggca
     4081 acggcgtgcc tctgcccctg gcaatggttg cggcgaccct ggtcaccgcc ggcgtcggcg
     4141 cggtggtcgg cgtgcccgcc ctgcggatga cgggcattta cctggccgtg gcgaccctcg
     4201 ccctggccgt gatcgtcgag aaggtgctgg agaaatggga gacggtgacc ggcggcctgc
     4261 gtggcctgcc cgtgcccaag gctcagtttc tcggcttcga cctcggcgct ccggccaact
     4321 tctactatct gtgtcttggg gtgcttgcgc tcggtctgct ggcggcgatg aacctgctgc
     4381 gcagccctac gggccgggcc atggtcgccg tgcgggactc cgaaacctcg gcccagagca
     4441 tgggcattca catcaaccgc atcaaggggc tggccttcgc cctgtcggcc ggcttcacgg
     4501 gtcttgcggg cgcgctgttt gcgcaccgga ttggctacct ctttccggac tcgttcaccg
     4561 tgctcacctc cattcagctg ctgctgatgg tcgtcgtggg cggcctcggc tcgctgcacg
     4621 gggtcgtgtt cggggccatc ttcatcggcc tgctgcccca ggggatcgcc ctgttcaggg
     4681 acgtggtgcc gccggcgatc gcccagatac cggggctgga gcccggcata ttcggcctca
     4741 ttctcgtgct cttcctgata ttcgaaccgc tgggtatata cggccgctgg gtcaagatcc
     4801 gccttttctt cgaggagttt ccgctctatc ggcgggcgac ctacaagcgg cagaagacgt
     4861 atctgcgcac ggagcgattg cggtgagcct ttttgccgcg gaggacgtcg cggtgagctt
     4921 cggcggcatc aaggccgtgc acgacctttc ctttgccgtg gaagacggcg aggtgttcag
     4981 catcgtaggc cccaacggcg cgggcaaaac gacgatcttc aacctcatca gccgaatcta
     5041 cgacgtggac caggggtccc ttgccttcga cggcacagag atcaccagat tgccggccca
     5101 cgccgttgcc gagctcggcg tcgcgcgcac gttccagaac accgagctgt tcgagcagga
     5161 aacggtgctg aaaaatctgc tgatcggctg tcacgtccat cgacgctccg gcctgcttgc
     5221 cgacattctc tttctgccgt ccgtgaaggc ccaggagctg acttttcgca agcgggtgga
     5281 ggacgtcatc gatctgctgg atctgcagag ctaccgtgat caggtgatcg gcaatcttcc
     5341 ctatggcgtt cgcaagatgg tggagatcgc cagggcgctg tgcctgggcc cgaagctgct
     5401 gctgctggac gagccttcgt ccggtctcaa cccggaggag accgaggacc tctccttctg
     5461 gctggaggac atcaacaacg atctcggcat caccatcatc atggtggagc acgacatgaa
     5521 tctcgtctcc cgcgcttcgg accgggtcat ggcgctggcg gacggggcgc tgctgaccat
     5581 cgggtctccg gccgacgtcc agcagcaccc ggacgtcctg cgggcgtacc tggggg
//
LOCUS       DBNHQR010000086         2468 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_642106, whole genome shotgun sequence.
ACCESSION   DBNHQR010000086 DBNHQR010000000
VERSION     DBNHQR010000086.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_642106"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..816)
                     /locus_tag="AC16VR_01915"
     CDS             complement(7..816)
                     /locus_tag="AC16VR_01915"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019821071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HIR6009937.1"
                     /translation="MEPSEATDTLIRLACFIGALVLFALLEAAFPRRQRQVSRKARWP
                     SNLGISALNQLVLRLVLPASAIALAVRLEQEGWGLLAQLTWPTWIELAAAVLILDLAI
                     YLQHRLYHAVPFLWRFHRMHHADLDFDVTTGIRFHPLSLLLSGLIKLGAVLIIGPSPF
                     AVLVFEVLLNATTMFNHSNLKLPRPFDRILRLVIVTPDMHRVHHSADPAETNRNFGFN
                     FPWWDRLFKTYRPQPALGHEAMTVGLEDFRSAGELRLDRMLSQPFRSGTGS"
     gene            1019..2359
                     /locus_tag="AC16VR_01920"
     CDS             1019..2359
                     /locus_tag="AC16VR_01920"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6009938.1"
                     /translation="MVTSRQEALVQRSQAVSGSVSRIFPADVTITGERYTSREFMELE
                     LDRLWPRVWNIGAWAREIPVAGDFVTHAIGRESILMVRQADGSVRAFHNVCPHRGNRL
                     VYSERGSVERFTCSYHGWQFDQAGTVVHVQDEEDFPRGNPCGKLTLAEIPCEVWAGFV
                     WYNMDAECASLAEFLGEVKDYVDLHDLSAARRIYYKVCEVGFNWKLLHDNFCESYHLP
                     ATHPQISDYYDDDYRNTDFELYETGHNLMKMKGSLPSLRYDEPFAINETLAADMQNWG
                     LDPAAFEGRAHAVREALQAQRRKLGPARGYRYFERLPASWLTDAYHFNLFPGTSLTVV
                     ADGAQMQRCEPHPTDPTRSIYEHWFLALKGADSDLVPGPEGMVPWEEAERRVVEYGQE
                     TLSEVADQDLERASGQQLGVQSRGFAGAYLSGQENRVQQFHNFIDRYLRDDIGS"
BASE COUNT          465 a          748 c          849 g          406 t
ORIGIN      
        1 attccgtcag ctgccggtac cgctccggaa gggttgagaa agcatgcggt cgagccggag
       61 ttcgcccgct gatctgaaat cctccaggcc caccgtcatg gcttcgtggc cgagcgcggg
      121 ctgaggccgg taggttttaa agaggcgatc ccaccacggg aagttgaagc cgaaattccg
      181 attcgtctcg gccggatcag cggaatggtg cacgcggtgc atgtcggggg tcacgatgac
      241 gagtcgcaga atccgatcga acgggcgggg aagcttcagg ttgctgtgat tgaacatggt
      301 ggttgcgttc agcagcacct cgaagacgag cacggcgaag ggtgacggtc cgatgatcag
      361 aaccgcgccg agcttgatga ggccggagag caacagtgag agcggatgaa accggatgcc
      421 cgtggtgacg tcgaagtcga ggtcggcatg gtgcatgcgg tgaaagcgcc ataggaaagg
      481 gaccgcgtga tagagccgat gctgcaggta gatggccaga tccaggatca gaaccgccgc
      541 cgccagctcg atccaggtcg gccatgtcag ctgagccaag aggccccagc cctcctgctc
      601 caggcggacg gcgagcgcga tcgcagaggc gggcagcacc agccgcagga ccagctggtt
      661 cagagcggag atgccaaggt tgctgggcca gcgtgctttg cgggacacct ggcgctgccg
      721 ccggggaaac gctgcctcca gcagggcaaa caaaaccagc gcgccgataa aacatgccag
      781 gcggatgagc gtatcggtag cttcgctcgg ttccatgggt gtaagaatgc tcgcattggc
      841 cccctggtgc cacttaaggc gtccgtcgcc tgacggccgg cgccgacggc cgccctcgct
      901 tttgcgctct ggataaatcg ctgtggtggc tgtaataatc gttggacgcg ttgacgcacg
      961 gcaagcggaa gagggttgtc gtgcaagcgg ccgcgccagg ttggagaagg ggagcgacat
     1021 ggttacgtcc agacaggaag cgctcgttca gcgcagccaa gccgtatcgg ggagcgtgag
     1081 ccggatcttt cccgctgacg ttaccatcac cggcgagcga tacacctccc gcgagttcat
     1141 ggagctggag ctcgaccgct tgtggccccg ggtctggaac atcggggcct gggcgcggga
     1201 gatcccggtg gcgggtgact tcgtcaccca cgcgatcggc cgggagtcga tactcatggt
     1261 gcggcaggcg gacggctcgg tccgtgcctt ccacaacgtc tgcccccacc gcggcaaccg
     1321 gctggtctac tcggagcggg gatccgtaga gcgcttcacc tgcagctatc acggctggca
     1381 gttcgaccag gccgggacgg tcgttcacgt gcaggacgag gaggactttc cccgggggaa
     1441 cccgtgcggg aagctcacgc tggcagagat cccctgcgag gtctgggccg ggttcgtctg
     1501 gtacaacatg gacgccgaat gcgcctcgct cgcagagttt ctcggcgagg taaaggacta
     1561 cgtcgacctg cacgatcttt cggctgccag gcgcatctac tacaaggtct gcgaggtcgg
     1621 cttcaactgg aagctgctgc acgacaactt ctgcgagtcg taccatctgc cggccaccca
     1681 cccgcagatc agcgactact acgacgacga ctaccggaac actgacttcg agctctacga
     1741 gaccggccac aacctgatga agatgaaagg ctcgctgccg tcgctgcgtt acgacgagcc
     1801 gttcgccatc aacgaaaccc tcgccgccga catgcagaac tgggggcttg accccgccgc
     1861 cttcgagggg cgcgcgcacg ccgtgcggga ggctctgcag gcgcagaggc gcaagctggg
     1921 gccggcgagg ggctaccgct acttcgagcg tctgccggcg tcctggctga cggatgccta
     1981 tcacttcaac ctctttcccg gcacctcgct caccgtggtg gcggacgggg cccagatgca
     2041 gcgctgcgag ccgcacccca cggaccccac acggagcatc tacgagcact ggttcctggc
     2101 gctgaagggc gcagactcgg acctggtgcc cgggccggaa gggatggtgc cctgggagga
     2161 ggccgagcgg cgggtggtgg agtacggcca ggagacgttg agcgaggtgg ccgatcagga
     2221 tctcgagcgc gcctcaggcc agcagctcgg agtgcagtcg aggggatttg cgggcgcgta
     2281 tctctcaggt caggagaatc gggtgcagca gttccacaac ttcatcgacc gctatctgcg
     2341 cgacgatatc ggttcctagc ggcacccgct gcgctggcag tcccgcgcgt ccgggcctgc
     2401 gtctcagaaa ccgaactcca gcttggggat gccgcgcgca tcctcgcagg cggcgcggaa
     2461 gtcgtagg
//
LOCUS       DBNHQR010000087         4183 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_769377, whole genome shotgun sequence.
ACCESSION   DBNHQR010000087 DBNHQR010000000
VERSION     DBNHQR010000087.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_769377"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1439
                     /locus_tag="AC16VR_01925"
     CDS             <1..1439
                     /locus_tag="AC16VR_01925"
                     /inference="COORDINATES: protein motif:HMM:NF023649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HIR6009939.1"
                     /translation="FHGKHKGPGALATGATACLLAAAATAKEFNITAEVVEGSFEPAS
                     YSPYAGRNFPSRLLWGDTHLHTSLSLDARAAGVILDPEAAYRFARGEEIRTTAGMKIR
                     IGQPLDFLVITDHSDAMGAMNEVVAGNPDLLRDPTVRDWHQRISKGGESAFEAALEVV
                     DSFTKGNTPDVLMSGTFAERIWNGVTETADAFNEPGRFTAMIGYEWTSTTDGNNLHRN
                     VLYRDGADSAKKVLPFTVIDSDNPEDLWTWMENYEATTGGEVLATAHNGNLSNGLMFP
                     DVNPATNRRLTESYAARRARWEPIYEVTQIKGDGEAHPLLSPNDEFADYETWDQGNLG
                     PNLKDDDMLQYEYAREALKKGLAHEVRLGVNPYKFGLQGATDSHTGLATAEEDNFFGK
                     HSQTEPTAARLATDVGHFENNVWPGWYQVASGYTGVWATENTREAIFDAMMRKEVYAT
                     KYFGTTAPEESPMTTQERAYTSPIWYTP"
     gene            1499..3433
                     /locus_tag="AC16VR_01930"
     CDS             1499..3433
                     /locus_tag="AC16VR_01930"
                     /inference="COORDINATES: protein motif:HMM:NF023649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HIR6009940.1"
                     /translation="MAGILTLLAACDPAGTEPTGTDTDAAPQPVTVGPAPSAMGDRRL
                     LWGDTHLHTSYSPDAYLMQNRSADPDTAYRYAKGLPVVHPYHKARIQIGTPLDFLVVS
                     DHGEFMGVVPMVLQGDPRVANTETGKRYRAMAEAGREIEVFGELIAQVNKIVPPDQDL
                     YNPDINRSVWGEIMEAADRHNEPGTFTAFMGWEWSSTPQGQNLHRVIVMRGDKDNGDE
                     FVPYTSLDSDRPEDLWAWLEETAEAAGTDFLAIPHNSNISGGLMFPLVNSDKEAVDAE
                     YAKLRMRWEPVVEMTQIKGDSETHPMLSPNDEFADYETYEHLIAAEGIEQQTMFTEGF
                     LGTLSDADRAVLESQEKRIVKNGDYARTGLLRGLVLEERGRGNPYKFGMIGSTDSHTA
                     MASAEEDNFHGKMAIDSTPGTKREDIIPGTPGWDMGAAGLAAVWATENTREGIFDAMK
                     RKEVYATTGPRIELRFFGGFDFAAADAQARDLASVGYGKGVPMGGDLTASPAGNAPSF
                     LVSAVKDPVGANLDRVQIVKGWVDAEGMPQEKIFDVALADGRTVGADGKVAPVGNTVD
                     VSTGHYTNAIGDPQLTVVWRDPEFDPGKRAFYYVRVLQIPTPRHTLYDAIALGISVEE
                     TGHPATIQERAYSSPIWYAP"
     gene            3505..>4183
                     /locus_tag="AC16VR_01935"
     CDS             3505..>4183
                     /locus_tag="AC16VR_01935"
                     /inference="COORDINATES: protein motif:HMM:NF024545.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR6009941.1"
                     /translation="MNKLLKEPLLHFLVLGLALFVLFDVVSDEKETSDSKLIRVDRET
                     LLTFIQFRTRAFEPSIAAQRLDGLSDQELKLLIDDYIREEALYREALALGMDKNDYVI
                     KRRMIQSIEFITRGFVTRAVEVSDDDVKAHYEANRNDYYVEPFVTFTHVFISGENRSE
                     AEVDALAAAELTELNARQIPFSAAPGRGERFPFFVNYVERDPEFVASHFGADMANEIF
                     ALEPDELV"
BASE COUNT          827 a         1397 c         1322 g          637 t
ORIGIN      
        1 catttcacgg aaaacacaaa ggccctggcg cgctcgcaac gggggcgacg gcctgcctgc
       61 tcgctgcggc ggcaacggct aaggagttca acatcaccgc cgaggtagtg gaggggtcct
      121 tcgagcccgc cagctactcg ccgtacgcgg ggcgcaactt ccccagccgg ctgctttggg
      181 gggataccca cctgcacacg agcctgtcgc tcgacgcccg ggccgccggc gtgattctcg
      241 acccggaggc cgcctaccgg ttcgcccggg gcgaggagat ccgcacaacg gctgggatga
      301 agatccgcat cggccaaccg ctggacttcc tggtgatcac cgatcactcg gatgccatgg
      361 gagccatgaa cgaggtggtg gcgggcaatc cggatctgct gagggacccc accgtgcggg
      421 actggcatca gcgcatctct aagggcggcg aatcggcttt tgaggcggcg ctcgaggtcg
      481 tcgactcgtt caccaagggc aacacccccg acgtcctcat gtccggcacg ttcgcagagc
      541 gcatctggaa cggggtgacg gaaaccgcag acgccttcaa cgagcccggc cgattcaccg
      601 ccatgatcgg ctacgagtgg acgtccacca cggacggcaa caacctgcac cgcaacgtgc
      661 tctaccggga cggcgccgat tcggccaaga aggtccttcc cttcaccgtg atcgacagcg
      721 acaacccgga agacctgtgg acgtggatgg agaattacga ggccaccacc ggcggtgagg
      781 ttctcgccac cgcccacaac ggcaacctga gcaacgggct gatgttcccg gacgtgaacc
      841 cggccaccaa ccgccggctc accgagtcat acgccgcgcg ccgagcgcgc tgggagccga
      901 tctacgaggt cacccagatc aagggggacg gtgaggcgca cccgctgctc tcgcccaacg
      961 acgagttcgc cgattacgag acctgggacc agggcaacct gggccccaat ctcaaggacg
     1021 acgacatgct gcagtacgag tatgcccggg aggcgctcaa gaaggggctg gctcatgaag
     1081 tccggctcgg cgtcaacccc tacaagttcg gcctgcaggg agcgacggac tcccacaccg
     1141 gcctcgccac cgccgaggag gacaacttct tcggcaagca ctcgcagacc gagcccaccg
     1201 cggcgagact ggccaccgac gtcggccatt tcgagaacaa cgtctggccc ggctggtatc
     1261 aggtggcgtc gggctacacg ggagtgtggg ccacggagaa cacccgggag gcgatattcg
     1321 acgccatgat gcgcaaggag gtctacgcca ccaagtattt cggcaccaca gctcccgagg
     1381 aatcccccat gaccacccag gaacgtgcat atacctcacc gatctggtac accccctgac
     1441 cgggaaagtc actcgaagga agcggaccat gaaaacaagg atgattgggg gcacttgctt
     1501 ggccggcatt ctcactctgc tggccgcctg cgacccggcc ggtaccgagc ccacgggcac
     1561 ggacaccgac gccgcgcccc aacccgtcac cgtgggcccg gccccgtcgg caatgggaga
     1621 cagaaggcta ctctgggggg acacccacct gcacacgtcc tactcaccgg acgcgtacct
     1681 gatgcagaac cgctcggcgg atccggacac cgcctaccgc tacgccaagg ggctgcccgt
     1741 ggtgcacccc tatcacaagg cgcgcatcca gatcggcacg ccgctggatt ttctggtggt
     1801 ttccgatcac ggcgagttca tgggcgtggt gcccatggtc ctgcagggag atccccgggt
     1861 cgccaacacg gagaccggca agcgctatcg ggcaatggcc gaggcgggcc gcgagatcga
     1921 ggtgttcggc gagctcatcg cccaggtcaa caagatcgtc ccaccggacc aggacctcta
     1981 caacccggac atcaaccgct cggtttgggg cgagatcatg gaagccgccg accgtcacaa
     2041 cgagcccggc accttcaccg cgttcatggg ctgggagtgg tcgtccacgc cccaggggca
     2101 gaacctgcac cgggtgatcg tcatgcgcgg cgacaaggac aacggcgacg agttcgttcc
     2161 ctataccagc ctggacagcg accggcccga ggatctgtgg gcgtggctgg aggagactgc
     2221 cgaggcggcc ggcaccgact ttctcgccat cccccacaac tcgaacatct ccggtgggct
     2281 catgttcccg ctggtgaaca gcgacaaaga agccgtggac gcggagtacg cgaagctgcg
     2341 catgcgctgg gagcccgtgg tggagatgac ccagatcaag ggcgactccg agacccatcc
     2401 catgctctca ccgaatgacg agttcgccga ctacgagacc tacgagcacc tcatcgctgc
     2461 cgaaggcatc gagcagcaga ccatgttcac cgagggcttt ctcggcacgt tgagcgacgc
     2521 ggaccgagcc gtgctcgaaa gccaggagaa gcgcatcgtc aagaatggcg actacgcccg
     2581 aacggggctg ctccgcgggc tggtgctcga ggagcgcggg cgcggcaacc cctacaagtt
     2641 cggcatgatt ggctcgacgg actctcatac cgccatggca tccgccgaag aggacaactt
     2701 ccacggcaag atggccattg actccacgcc gggaaccaag cgcgaagaca tcattcccgg
     2761 cacgccgggc tgggacatgg gcgcggcggg cttggcagcg gtgtgggcca cggagaacac
     2821 ccgcgagggg atcttcgacg ccatgaagcg caaggaggtc tacgccacca cggggccgcg
     2881 catcgagctg cgcttcttcg ggggtttcga cttcgccgcc gctgacgccc aggcccggga
     2941 tctggcctcg gtggggtacg ggaaaggggt ccccatgggc ggcgatctga ccgcgtcacc
     3001 cgcgggcaac gcgccttcgt ttctggtgag cgccgtgaag gatccagtgg gcgccaatct
     3061 cgatcgggtc cagatcgtca agggctgggt ggacgccgag gggatgcccc aggagaagat
     3121 cttcgacgtg gcgctggcag acggccgaac ggtgggcgca gatgggaaag tcgcccccgt
     3181 gggcaatacg gtggacgtct ccaccggcca ttacaccaac gccatcgggg atccccagct
     3241 caccgtggtc tggagggacc cggagttcga ccccggcaag cgcgccttct actacgtgcg
     3301 ggtgctgcag attcccactc cccgacacac gctctacgac gccatcgcgc tcgggatatc
     3361 ggtggaagag acggggcatc cggcgaccat tcaggaacgg gcgtactcct cgcccatctg
     3421 gtacgcaccc tgacgacgtt tggactctga ccccccgctg ccgcccaccc ggcggcagcg
     3481 agcggcgtca ttttagagag agccatgaac aagcttctca aagaacccct tctgcacttc
     3541 ctcgttctgg ggctggccct gttcgtgctg tttgacgtgg tctccgacga gaaggaaacg
     3601 tcagacagca agctcatcag ggtggatcgc gagaccctgc tgaccttcat tcagttccgg
     3661 acccgcgcct tcgagccgag catcgccgcc cagcggctcg acggcctttc cgatcaggag
     3721 ctcaagctgc tcatcgacga ctacatccgc gaggaggccc tgtaccgcga agcgctcgcc
     3781 ctgggcatgg acaagaacga ctacgtgatc aagcgccgca tgatccagtc catcgagttc
     3841 atcaccaggg gcttcgtcac ccgggccgtg gaggtgtccg acgacgacgt gaaggcccat
     3901 tacgaggcca atcggaacga ttactacgtg gaaccgtttg tcacgttcac gcacgtcttc
     3961 atatccggtg agaaccgcag cgaagccgag gtcgacgcgc tggctgccgc cgagctcacc
     4021 gagctcaatg cccgccagat accgttcagc gccgcccccg ggcgagggga gcggttcccc
     4081 tttttcgtca actacgtgga gcgcgacccg gaattcgtcg ccagccactt cggggctgac
     4141 atggcgaacg agatcttcgc cctggaaccc gacgagctcg tct
//
LOCUS       DBNHQR010000088         4470 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_388354, whole genome shotgun sequence.
ACCESSION   DBNHQR010000088 DBNHQR010000000
VERSION     DBNHQR010000088.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4470)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_388354"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="AC16VR_01940"
     CDS             <1..512
                     /locus_tag="AC16VR_01940"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6009942.1"
                     /translation="HGQNGEGNAALNAPRIAGQEPWYISRQLAAYRAGHRGTAPGDIY
                     GMQMRPMAMTSADPATEANLIEYIGTLSAAPATATIEGDAAAGQNGYAVCVACHGANG
                     EGNQALGGPKLAGQNDWYLVRQIKNYQSGLRGYDPADTYGMQMKPMAGVLADDKAIND
                     VVAYINTLR"
     gene            515..1561
                     /gene="alr"
                     /locus_tag="AC16VR_01945"
     CDS             515..1561
                     /gene="alr"
                     /locus_tag="AC16VR_01945"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR6009943.1"
                     /translation="MRGRLHVDLAALAANYRSFSSAAEAAGGTAGGVVKADAYGLGAA
                     RVAATLQSEGCAEFFVATVEEGAALRAILPEAIIYVFEGATGATAEVLASNDLVPVLN
                     HRGQLEAWQAYRERPAAVHVDTGMNRLGFPADVDAVQFAGMNLSLLLTHLACADVPDD
                     PMNHEQVERFRRAATSFPGVRTSIGNSAGMLADASCCGDLGRPGVGLYGGNPFSDRRN
                     PMRRVAALQGQVLQVRTCSAGSPLGYGATYVAPADLRVAVVGLGYADGLPRLISGRGC
                     AAVGGRRCALLGRVSMDLTLVDVTGLEVQAGDWIEMFGDTVSVDEVAGWAETIAYEIL
                     TGIGDRVERVYSGD"
     gene            complement(1546..2187)
                     /locus_tag="AC16VR_01950"
     CDS             complement(1546..2187)
                     /locus_tag="AC16VR_01950"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6009944.1"
                     /translation="MLIALTAGAALAETAVDAGVERGGEAGGAPGSIQSAAELLVADE
                     AALGCHREVTAADPNPEPCDVFLRLTADSLDETLRQAAAHNNRGLILTRLGGLEAALA
                     DFEAALALAPGLGAAEVNRGTALLHMGLYPDALKAFENVIVNDEEHRATARFNRSFVY
                     RALGELDLAAADLASAAQTDLPVSRSREPIPLQAGPAAEPLDRPMELEGQSPE"
     gene            2352..3737
                     /locus_tag="AC16VR_01955"
     CDS             2352..3737
                     /locus_tag="AC16VR_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HIR6009945.1"
                     /translation="MADPLHNQFPPGSLEARDLEHLLHPTTNLEQHHKVGPRVHDRAE
                     GVYLWDSQGRQYLEGMAGLWCTALGYGEEELVSAAAQQMRKLSYSQLFGGRSNEPSIL
                     LAERLKAMAPFDAGRVFFGLSGSDANDTQIKLMWYYHNAIGKPEKKKIISRMRGYHGV
                     TVAAGSLTGLPAFHAHFDLPIPGVLHVDAPHYYREAKGGETETEFVDRLVGDLEELIL
                     GEGPETIAAFIAEPVQAAGGVIVPPPGYFAKVQALLARHDILFIDDEVICGFGRTGRA
                     FGAQTFGIEPTTMSVAKGVSSAYLPLSAVIIPEFMYEPLVELSGSVGTFGHGFTYSGH
                     PVCAAVALRTLELMEEREIFAHAAAVSEPFQERLALLADHPLVGDARGVGLLGAVELV
                     ADKDTRTPFPPGQGVGAHCQERCESHGLILRALGDVVAICPPLIISEDQVDELFAKLE
                     RALDDTFAWVH"
     gene            3828..>4470
                     /locus_tag="AC16VR_01960"
     CDS             3828..>4470
                     /locus_tag="AC16VR_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HIR6009946.1"
                     /translation="MAILQSNIDTGAAAFAANAKHHRELADELRTLAGRIVEGGDARS
                     RERHVGRGKLLPRDRLSRLLDPGSPFLEVGQLGGYELYDDWIPSGGIVTGIGRVSGTQ
                     CMVVVNDATVKGGTYYPITVKKHLRAQEIALENGLPCVYLVDSGGAFLPMQAEVFPDR
                     DHFGRIFYNQANLSARGIPQIAVVMGSCTAGGAYVPAMCDQAIIVRGQGTIFLG"
BASE COUNT          709 a         1433 c         1532 g          796 t
ORIGIN      
        1 gtcacgggca gaacggcgag ggcaacgcgg ccctgaacgc gcctcgcatc gccggtcagg
       61 agccctggta catcagccgt cagctggccg cttaccgggc cgggcatcgc gggacggcgc
      121 ccggagatat ctacggcatg cagatgcggc ccatggccat gacctcggcg gacccggcaa
      181 ccgaggccaa tctcatcgag tacatcggaa cgctttccgc ggcgcctgcc acggcgacca
      241 tcgaaggcga cgccgctgcc ggccagaatg gctacgcggt gtgcgtggcc tgtcacggtg
      301 cgaacggcga gggcaatcag gccctgggag ggcctaagct ggccggccag aacgactggt
      361 acctcgtgcg ccagatcaag aattatcaga gcggcctgcg cgggtacgat cccgccgata
      421 cctacggcat gcagatgaag cccatggctg gcgttctggc tgacgacaaa gccatcaacg
      481 acgtggttgc gtacatcaac acgctgcgct gatcgtgcgc ggccggctgc acgtcgacct
      541 ggctgcgctc gccgcaaatt accggtcgtt ctccagcgcg gctgaggccg caggcggaac
      601 cgcgggtggg gtcgtcaagg ccgacgccta cggtctgggc gccgcgaggg tggcggcaac
      661 tctgcagtct gagggttgcg cggagttctt cgtagccacc gtggaggaag gcgccgcgct
      721 gcgggccatc ctgccggaag ccatcattta cgttttcgaa ggcgccacgg gggcaaccgc
      781 ggaagtgctg gcatccaacg acctcgttcc cgtgctcaac catcggggtc agctcgaagc
      841 ctggcaggct taccgggagc gtcctgccgc cgtccatgtg gacaccggta tgaaccggct
      901 ggggttcccc gcggacgtcg atgcggtgca gtttgcgggg atgaacttaa gccttctgct
      961 cacccatctg gcctgcgcgg acgtgcccga tgaccccatg aatcacgagc aggtcgagcg
     1021 cttccggcgc gccgccacgt cgtttccagg tgtacgcacg agcatcggca actccgcggg
     1081 catgttggcc gacgcgtcgt gctgcggtga ccttggccgt cccggtgtcg ggctctacgg
     1141 cggcaatccc ttctccgacc gacgcaaccc catgcgccgg gtggccgccc tccaagggca
     1201 ggtcctgcag gtgaggacgt gctcggctgg cagcccgctg ggctatgggg cgacctacgt
     1261 tgcgcccgcc gatctgcggg tggccgtggt ggggctgggt tacgcggacg gcctgccgag
     1321 gctgatatcg ggccgaggct gcgctgcggt gggtggtcgg cgatgcgctt tgctcggccg
     1381 ggtttccatg gacctgaccc tggtggacgt aaccgggctc gaggttcagg ccggcgactg
     1441 gatcgagatg tttggcgaca cggtgtccgt cgacgaggtt gccggctggg cggagaccat
     1501 cgcttacgag attctcaccg gcatcggcga tcgcgtggag cgcgtctatt ccggggattg
     1561 accctcgagc tccattggcc ggtccagggg ctctgcggcg ggtcccgctt gcaggggtat
     1621 cggctcgcgg cttcggctca cgggcaggtc cgtctgcgcg gcagatgcca ggtcggcggc
     1681 ggcgaggtcc agctcgccca gggcccgata cacgaaggag cggttgaacc gtgccgttgc
     1741 gcgatgctcc tcatcgttga cgatgacgtt ctcgaacgct ttgagcgcgt cggggtagag
     1801 ccccatgtga aggagcgcgg ttccgcggtt gacctctgcc gcgcccaacc caggggccag
     1861 agcgagagcc gcttcgaagt cggctagcgc cgcctccaac cctcccagcc tggtcaggat
     1921 cagacctcgg ttgttgtgcg ccgctgcctg ccggagagtc tcgtccaagc tgtcggcggt
     1981 cagtctcaga aaaacgtcgc agggctcggg attggggtct gctgccgtga cttcgcgatg
     2041 gcagccgagt gccgcttcgt ccgccacgag cagctcggcc gccgactgta tcgagccggg
     2101 cgcgccgcca gcttccccgc ctcgctccac gcccgcgtcc acagcggtct cggccaacgc
     2161 agcacctgcc gtcagagcaa tcagcatcga gaaggtgata atcgcggttc gggcgagcat
     2221 gaggcgcagt gtaacgcaat ggcggcaggc gcagcgcgga cgcagcgcgg gcgctgcgac
     2281 cgcgctgctt tgccgtagcg ctcggacccg ccgccccgta agcttcgcct cccgcctgtc
     2341 gggagacgtt catggccgat ccgctgcaca atcagtttcc gcccggctcc ctggaggcca
     2401 gggatctgga gcaccttctc caccccacga cgaatctgga acagcatcac aaggtgggtc
     2461 ctcgtgtcca cgaccgggcc gaaggcgtct atctctggga ttcccagggc cgtcagtatc
     2521 tcgaaggcat ggcgggcctt tggtgcacgg cgctcggcta cggggaggaa gagctcgtca
     2581 gcgcggccgc gcagcagatg cgcaagctca gctactcgca gctgttcggc ggccgcagca
     2641 acgagcccag catactcctg gccgagcggt tgaaagccat ggcccccttc gatgccggta
     2701 gagtcttctt cggtctctcg ggcagcgatg ccaacgacac ccagatcaag ctcatgtggt
     2761 actaccacaa tgccatcggc aagccggaaa agaagaagat catcagccgc atgcgcggct
     2821 atcacggcgt gacggtggcc gccgggtccc tgaccggtct tcccgccttc cacgcgcact
     2881 tcgatctgcc gatcccgggg gtgctgcacg tcgatgctcc acactactat cgcgaagcga
     2941 agggcggcga gaccgagacc gagttcgtcg accggctggt cggcgatctg gaggagctga
     3001 ttcttggcga gggccctgaa accattgccg cgttcatcgc ggaacccgtt caggccgccg
     3061 gcggggtgat cgtgccgccg cccggctact tcgccaaggt gcaggcgctg ttggcccgtc
     3121 atgacatcct cttcatagac gacgaggtca tctgcggctt cggccgcacg ggccgcgcgt
     3181 tcggtgcgca gacgttcggg atcgagccga ccaccatgag cgtggcgaag ggcgtctctt
     3241 cggcctacct gccgctaagc gccgtgatca ttcccgagtt catgtacgaa ccgctggtgg
     3301 agcttagcgg gagcgtggga accttcggcc acggctttac ctattcgggc catccggtgt
     3361 gtgcggcggt tgcgcttcgg acgctagagc tcatggagga gcgcgagatc tttgctcacg
     3421 cagcagccgt gtcggagccg ttccaggaac gtctggcgtt gctggccgac cacccactgg
     3481 tgggggacgc gcgcggggtc gggctccttg gcgcggtgga gctcgtggcg gacaaggaca
     3541 cccgcacgcc ctttcctccc gggcagggtg tgggcgcgca ttgccaggaa cgttgcgagt
     3601 cccacgggtt gatcctccga gcgctcggag acgtggtggc catctgcccg ccgctgatca
     3661 tatccgagga tcaggtcgac gagctcttcg cgaaactgga aagggcgctg gacgacacgt
     3721 tcgcctgggt tcactgaaca gcgagcgttc gcgtcacagc tgcggcgcct tggcaggcag
     3781 ttcattgggt tgacgtcgtt gtgtatcttt gccgtctggg gggagttatg gccattctgc
     3841 aatcgaatat cgataccggc gccgccgcgt tcgccgccaa cgcgaagcac caccgcgagc
     3901 ttgccgatga gttgaggacg cttgccgggc gcatcgtcga gggcggggac gcccgctcca
     3961 gggagcgtca cgttggtcgc ggcaagcttc tgccgcggga ccgcctcagc cgtctgctgg
     4021 atccgggctc accatttctg gaggttggcc agcttggcgg ctacgagctt tacgacgact
     4081 ggattcccag cggcggcatc gtcaccggca tcggcagggt ctcgggcacg cagtgcatgg
     4141 tggtggtgaa tgacgccacc gtcaaaggcg gaacgtatta cccgattacc gtcaagaagc
     4201 acttgagggc gcaggagatc gcgctggaga atggcttgcc gtgcgtctat ctggtggatt
     4261 ccggcggcgc gttcctgccc atgcaggcgg aggtctttcc cgaccgggat cacttcggtc
     4321 ggattttcta caaccaggcc aatctctctg ctcgcggcat cccccagatc gcggtggtga
     4381 tgggatcctg cacggcgggg ggcgcttacg tgccggccat gtgcgaccag gccatcatcg
     4441 tccggggcca ggggacgatc tttctcggcg
//
LOCUS       DBNHQR010000089         4316 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_325614, whole genome shotgun sequence.
ACCESSION   DBNHQR010000089 DBNHQR010000000
VERSION     DBNHQR010000089.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4316)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_325614"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="AC16VR_01965"
     CDS             <1..725
                     /locus_tag="AC16VR_01965"
                     /inference="COORDINATES: protein motif:HMM:NF017591.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HIR6009947.1"
                     /translation="VYECDPLGLRQPVALPALGRFQHEAAVVDPLTGTVYMSEDEPDG
                     RLYRLRPTVPRHLERPDLSAGVLEVASLAPDGSGSGSVVWLRVPDPEAGRKPTRWQVP
                     ESTGFDGGEGMSYCDGVVYLATKGDNRVWALDLGEDRLEVIYDQATAATPILGGVDNV
                     ETNASGDVLVAEDGDDMQIVVLPREGEPVALLQVTRQGGSEITGLAFTPDMSRLYFSS
                     QRGFSGRVTGGITYEVTGPFVG"
     gene            743..1816
                     /locus_tag="AC16VR_01970"
     CDS             743..1816
                     /locus_tag="AC16VR_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017639523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA nucleotidyltransferase/poly(A)
                     polymerase-related protein"
                     /protein_id="HIR6009948.1"
                     /translation="MDVYLVGGAVRDELLGRPVRERDWVVVGASPEAMLKLGFRQVGR
                     DFPVFLHPDTSEEYALARTERKTGPGHKGFECHAGPDVTLEQDLERRDLTVNAIARAS
                     DGSLIDPFGGRSDLEAKVLRHVSVAFSEDPLRVFRVARFAAQLDDFTVAEETRALMAE
                     LAGSGALAELSAERVWQELVKALGSKAPLRFFTVLASVGGLSTWFAELAEVEITFPEA
                     IEDALGRFAALGWHLAPEAVDTLCERLRAPRRHRRAMGHIASHGVVLAAWETAEAGSL
                     CAALKSVGAFKAGDDPEMAVRVSAACAGRNPEPLLELAASIRQEVTTASVGGSLTGAA
                     LGEAIDQARTQAIRQAQRSSVGR"
     gene            complement(1868..2221)
                     /gene="folB"
                     /locus_tag="AC16VR_01975"
     CDS             complement(1868..2221)
                     /gene="folB"
                     /locus_tag="AC16VR_01975"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:NF014233.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HIR6009949.1"
                     /translation="MDSVFVRNLTVSTIVGVYPEERTRPQRVIVSFELFADTRAAAQS
                     DDLEDALDYGEAASRVAEFVRGSRFELIEALADGIAALLLEGYPAARVVVEVLKPGAV
                     TDAETVGVKIERSRA"
     gene            complement(2225..3247)
                     /gene="tsaD"
                     /locus_tag="AC16VR_01980"
     CDS             complement(2225..3247)
                     /gene="tsaD"
                     /locus_tag="AC16VR_01980"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIR6009950.1"
                     /translation="MLVLGIETSCDETGVALYDGVEGLLADALHSQVEVHAAYGGVVP
                     ELASRDHIRKLVPLVRKTLDDSGRSLADVDGVAYTSGPGLMGALLVGAGFARSLAWSL
                     GKPAVAVHHMEAHLLAPLMNPDTAPPMPFIALLVSGGHTQLVLVNAIGDYELLGESVD
                     DAAGEAFDKAAKMLDLGYPGGPNLARAAEGGDPHRFTFPRPMTDRPGLDFSFSGLKTY
                     TLNTTRDLAPMSDADRADVARAFEEAVVDTLYIKCRRAVEQTGITHLVMAGGVAANAR
                     LRRKLSEDLRAAVYYPPARLCTDNGAMIAYAGCLRLQAGERAPLAIETRARWPIVELS
                     RPGPPA"
     gene            3355..3570
                     /gene="rpsU"
                     /locus_tag="AC16VR_01985"
     CDS             3355..3570
                     /gene="rpsU"
                     /locus_tag="AC16VR_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HIR6009951.1"
                     /translation="MPSVKVKENEPFDVALRRFKRSCEKAGVLSEVRRREYYEKPTSV
                     RKRKAAAAVKRHLKKLQREARRFEKSF"
     gene            3593..4060
                     /locus_tag="AC16VR_01990"
     CDS             3593..4060
                     /locus_tag="AC16VR_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HIR6009952.1"
                     /translation="MSDLPISELRQAITAATTAAMKARDKQRLAVLRLVNADIKRAEI
                     DERRAASTDAEVLAVLNRMLKQRQDALEQYTQAGRKDLADQESFEIDLIGEFMPEQLS
                     EADLASAIDQAIAEANAESMRDMGKVMGLLKDRLQGRADMGAVSGRVKALLAG"
     gene            4184..>4316
                     /locus_tag="AC16VR_01995"
     CDS             4184..>4316
                     /locus_tag="AC16VR_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015502517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR6009953.1"
                     /translation="MIPVLLAGGTGSRLWPLSRELFPKQFHALLGDDSLLQSTLKRAD
                     "
BASE COUNT          715 a         1402 c         1482 g          717 t
ORIGIN      
        1 gggtatatga atgcgacccg ttgggattgc ggcaaccggt ggcgctgccg gcgctgggac
       61 gattccagca cgaggctgcc gtggtcgatc cgctcaccgg taccgtctac atgagcgagg
      121 atgagcccga cgggcgcctc taccgcctcc ggcccacggt gcccagacat ctcgagcggc
      181 ccgatctgag cgctggcgtc ctcgaggtcg cttcactcgc tccggacggc tccggctccg
      241 gctcggtggt gtggctgcgc gttccggacc ccgaagcggg ccgcaagccc acccgctggc
      301 aggtgcctga aagcaccggc ttcgacggcg gcgaggggat gagctactgc gacggtgtgg
      361 tctatctcgc gaccaagggc gacaaccggg tctgggctct cgatctcggc gaggatcgcc
      421 tggaggtgat ctacgatcag gccacggccg caacgcccat acttggcggc gtcgacaacg
      481 tggaaaccaa tgcatccggc gacgtgctgg tggccgaaga cggcgatgac atgcagatcg
      541 tcgttctccc ccgggagggc gagccggttg ccctgctgca ggtcactcgc cagggcggat
      601 cggaaatcac cggcctggcg ttcacgcccg acatgtcacg cctttacttc agctcccagc
      661 gcggcttttc cggtcgggtg acgggcggca tcacttacga ggtcaccggc ccctttgtgg
      721 gctgactttc tcagaatgag ccatggacgt atatctggtc ggcggcgcgg tccgcgatga
      781 gctgctcggc cgcccggtgc gcgagcgcga ctgggtggtg gtgggcgcct cccccgaggc
      841 gatgctgaaa ctcggctttc ggcaggtggg ccgtgatttc ccggtctttc tgcacccgga
      901 caccagcgag gagtacgcgc tggcccgcac cgagcgcaag acggggccgg ggcacaaagg
      961 tttcgagtgt cacgccgggc ccgacgtcac tctggagcag gacctggagc gccgcgacct
     1021 caccgtgaac gccatcgcgc gggcgtcgga cggctccctg atcgatccct tcggcggtcg
     1081 atcggacctg gaagcgaaag tgctcaggca cgtctcggtc gctttctccg aggatccgct
     1141 ccgggtattt cgggtggccc ggttcgccgc ccagctcgat gatttcaccg tcgccgaaga
     1201 gacccgggct ctcatggccg agctcgccgg cagcggcgcg ctggcggagc tctccgcgga
     1261 gcgggtctgg caggagctgg tcaaggcact gggatcgaag gcgcccctgc gctttttcac
     1321 cgttctggca tccgtgggcg gcttgtcgac gtggttcgcc gagctcgccg aagtcgagat
     1381 caccttcccg gaagcaatcg aagacgcgct cggtcggttc gccgcccttg gctggcatct
     1441 cgccccggag gcggtagata cgctatgtga acggcttcgg gcgccgcgcc gacaccgccg
     1501 ggccatgggc cacatcgcct ctcacggggt ggtacttgct gcctgggaga ccgccgaggc
     1561 gggaagcctg tgcgcggcgc tgaagtccgt gggggcattc aaggcgggtg acgatccaga
     1621 gatggcggtg cgcgtttcgg ccgcctgcgc ggggcgcaat cccgagccgc tgctcgagct
     1681 ggcggcctcg attcgtcagg aggtgacgac ggccagcgtt ggaggctccc tgaccggagc
     1741 cgctctgggc gaggccatcg accaggcgcg cacgcaggcc attcgccagg cccagcgttc
     1801 gagcgtcggc aggtaggccg ggtcgagacg tgcctcttgc tgatgtcttg attcgttggg
     1861 cgacaggtca ggcccgcgaa cgctcgattt tcacgcccac cgtctccgcg tctgtgaccg
     1921 ccccgggctt gagcacttcc accaccacgc gcgcggcggg atagccttcc agcagcagcg
     1981 ccgcgatgcc gtcggcgagc gcttcgatga gctcgaagcg gctgccgcgg acgaactccg
     2041 cgacccggct cgccgcttcg ccgtagtcga gggcgtcttc caggtcatcg ctttgggcgg
     2101 ctgcccgggt atcggcaaac agctcgaagg agacgatcac cctttgaggc cgggtgcgtt
     2161 cttcggggta gacgcccacg atggtggaga cggtcaggtt cctgacgaag acgctgtcca
     2221 tgggtcaggc cggcgggccg gggcggctga gctcgacgat gggccagcgc gcacgggtct
     2281 cgatggcaag gggcgctcgc tcgcccgcct gaaggcgcag gcagccggcg taggcgatca
     2341 tcgctccgtt gtcggtgcac agccgcgccg gggggtagta gacggcggcc cgcaagtctt
     2401 cgctgagctt tctgcgcagc cgggcgttgg ccgccacgcc gccggccatt accagatggg
     2461 tgatgcccgt ctgctccacc gcccggcggc acttgatgta gagggtatcc accacggcct
     2521 cctcgaacgc gcgcgcgacg tcggcgcggt ctgcgtcgct catgggggcc aggtcgcggg
     2581 tcgtgttcag ggtgtaggtc ttcaatccgc tgaagctgaa gtcgagcccg gggcggtcgg
     2641 tcatgggtct cgggaacgtg aatcggtgcg gatcgccccc ttccgcggcg cgcgccaggt
     2701 tgggacctcc gggatagccg agatccagca tcttcgccgc cttgtcgaag gcctccccgg
     2761 cggcgtcgtc cacggactca ccgaggagct cgtaatcgcc gatcgcgttg accagcacca
     2821 gctgggtgtg tcctccggaa acgagcaggg cgatgaacgg cataggcggg gccgtgtccg
     2881 gattcatcag cggggccagc aggtgcgcct ccatgtgatg caccgcgacg gccggtttgc
     2941 ccagcgacca ggccaggctc ctcgcaaaac ccgcgcccac cagcaacgcg cccatgagcc
     3001 cggggccgga cgtgtaggcg acgccgtcaa cgtccgcgag ggagcgtccg gaatcgtcca
     3061 gcgtcttgcg gacaagagga accagcttgc ggatgtggtc tctggaggcc agctcgggca
     3121 ccacgccgcc gtaagcggcg tgcacttcca cctggctgtg cagagcgtcc gccagcagcc
     3181 cctcgacacc gtcgtagagc gcgacgcccg tctcatcgca ggaagtttcg attccgagga
     3241 ccagcatggg cggatggtaa cgattccggc gttttgcaaa aaccgtccgg cgccagtaaa
     3301 atccgcgccc ttttcagcaa caaccgtcag gttcgagggg atttcgcgca caacatgccc
     3361 agcgtaaaag tcaaagagaa tgagccgttc gatgtcgctc tcaggcgatt caagcgttcc
     3421 tgcgagaagg ccggcgtgct ttccgaggtg cggagacgag agtattacga gaagccgacg
     3481 tcggttcgaa agcgcaaggc ggcggcggca gtcaagcgcc atctgaagaa gctgcagcgg
     3541 gaagcccggc gcttcgaaaa gtccttctga caccgcacga caggggaacg ccgtgtccga
     3601 tctccccatc tccgagttaa ggcaagccat caccgcggcc accacggccg ccatgaaggc
     3661 gcgcgacaag cagcgcctgg cagtgctgcg cctggtcaat gcggacatca agcgagcgga
     3721 gatcgacgag cgccgtgcgg cctccaccga cgccgaagtg ctggccgtgc tcaaccgcat
     3781 gctcaaacag cgtcaggatg ccctggagca gtacacccag gccgggcgca aggatctggc
     3841 cgaccaggag agcttcgaga tcgacctgat cggcgagttc atgccggagc agctctccga
     3901 ggcggatctc gcgagcgcca tcgaccaggc catcgccgag gccaatgcgg aatccatgcg
     3961 cgacatgggc aaggtcatgg gcctgctgaa agaccggctt cagggccgcg cagacatggg
     4021 cgcggtcagc ggtcgcgtca aagcgctttt agccggttag tcagcaggtt agttggcagc
     4081 gccggggcct cacttacaaa tccgcccacg gtgataatcc gaggatctgg tatcttggcg
     4141 cggcaatgcc gtcgtgcgga gtgacgagcg agcgatggaa aaagtgattc ccgtacttct
     4201 ggccggagga accggcagcc ggctctggcc gctctccaga gagcttttcc ccaagcagtt
     4261 ccacgccctt ctcggcgacg actcccttct gcagtcgacg cttaagcggg cagacc
//
LOCUS       DBNHQR010000090         4390 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1655665, whole genome shotgun sequence.
ACCESSION   DBNHQR010000090 DBNHQR010000000
VERSION     DBNHQR010000090.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4390)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1655665"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            254..730
                     /locus_tag="AC16VR_02000"
     CDS             254..730
                     /locus_tag="AC16VR_02000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6009954.1"
                     /translation="MPSRRELIALTEGEIRDYLAEQKTLIIVSNGKDGYAHPVPMWFW
                     TDPDGCLYCTTFSKAQKVLNFRRDPKASLLVESGDEYTELKSVLIYAKAEVIEEFDAV
                     REALVNISTKGRGDVSEAERAQIRDAVGKTAEKRVLLKFTPERYVSWDHAKLGGRY"
     gene            complement(741..1445)
                     /locus_tag="AC16VR_02005"
     CDS             complement(741..1445)
                     /locus_tag="AC16VR_02005"
                     /inference="COORDINATES: protein motif:HMM:NF015605.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6009955.1"
                     /translation="MQGLPEVSIGALLVAFLPSLALVAVMRAWSLQAGQALIANIRML
                     VQLLAIGYVLVFVFETENPWIVAAVLVMMMGVAAWIALRPLQSRSARKYLQSLTAISI
                     CGLAMLALVSQLVIDLPRWFEPRFVVPLAGMIFANAMNTVSLAAERFESEQAREVEYA
                     EARTTALDAALIPQVNALLAVGLVSLPGMMTGQILSGVDPLIAVRYQIVVMCMIFGSS
                     GLAAVAYLFAQRPRGA"
     gene            complement(1473..1859)
                     /locus_tag="AC16VR_02010"
     CDS             complement(1473..1859)
                     /locus_tag="AC16VR_02010"
                     /inference="COORDINATES: protein motif:HMM:NF013304.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HIR6009956.1"
                     /translation="MTTPLWCLLIVMLMPIVLAGVGGYFRNKQLGTLDNDDPREQIKS
                     LTGAGSRAYAAQQNAWEALALFTAAALTAHVLGADPERSAQLAVAFVVFRVLHGVCYI
                     GGWATLRSLMFMGSLVSLIWLFVLGA"
     gene            complement(1869..3230)
                     /gene="ahcY"
                     /locus_tag="AC16VR_02015"
     CDS             complement(1869..3230)
                     /gene="ahcY"
                     /locus_tag="AC16VR_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003414325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HIR6009957.1"
                     /translation="MTDYKVADMSLADFGRREINLAEAEMPALMALRNRYADQQPLAG
                     AKVIGCIHMTVQTAVLIETLVALGAEVRWSSCNIFSTQDHAAAAMAAAGIPVFAWKGE
                     TEEEYWWCIEQTILKDGKPWDANLLLDDGGDLTEVIHEKYPEMLDSIHGVSEETTTGV
                     HRLYEMIDRGELKVPAINVNDSVTKSKNDNRYGCRHSLNDAIKRGTDMLMAGRRALVI
                     GYGDVGKGSAQSLRQEGMIVRVVEIDPICAMQACMDGFEVVSPYLGGENTGRIENIDT
                     RLLHDTDLIVTCTGNADVCDASMLSTVKAGAVVCNIGHFDNEIDTAFMREHWRWEMVK
                     DQVHQVFRSDDRHDYLILLSEGRLVNLGNAMGHPSRVMDGSFANQVLAQMHLYEQRWA
                     DQPENQRAPITVEVLPKKLDEEVAELMVAGFGGVVTRLTAEQAKYIGVDESGPFKPES
                     YKY"
     gene            complement(3291..4247)
                     /locus_tag="AC16VR_02020"
     CDS             complement(3291..4247)
                     /locus_tag="AC16VR_02020"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HIR6009958.1"
                     /translation="MGQTAGQQANLDEVVEVAKAVGDHLRASILRVLSDGSYSVTELC
                     HLFQIPQPALSHHLKVLLTANLVAKRREGNNVYYRRCTEPDDGLRQRLFETVDGTPLG
                     AALAERAAEIHAERSQRSNNFFAENAHRFADQQALICEAQVYVPAIVELLDHAGVDSG
                     PIIEVGPGGGQLLATLAGSFDDLIGIDSSSTMLAQAAERLDGLPNVKLRQKDFTELPA
                     IRRYRAVIAAMVVHHLASPQRFFRHASRILRHGGLLIVAELDRHEQEWVTDACGDLWL
                     GFEPGELEQWAAQAGLAPVESQYLAQKNGFQIQIHGYARNGT"
BASE COUNT          770 a         1384 c         1439 g          797 t
ORIGIN      
        1 tgaggcggcc accagggcct gccccagaac ctgcccccca tagacccgct gccaaccctc
       61 gctggggctg gtcgcgcggt agtggttctt ctcgatctcc tccagatcga ggagatcgat
      121 gagatcggtg acggtctggt cctggctcat tggcggcttc tggcgggctt ggagggagcg
      181 ctattgtagt cagccgaccc gccggggaga atggcgatcg ccgctttaac cgccgccccg
      241 aggagttgcc cggatgccgt cacgccgcga gctgatcgcc ctgaccgagg gagagatcag
      301 ggactacctg gccgagcaga agacgctgat catcgtgtcc aacggcaagg acggctacgc
      361 ccaccctgtg cccatgtggt tctggaccga cccggatggc tgcctctact gcaccacgtt
      421 cagcaaggcc cagaaggtgc tcaacttccg gcgcgacccg aaggcgtccc tgctcgtgga
      481 gagcggggac gagtatacgg agctcaagag cgtgctgatc tatgccaaag cggaggtgat
      541 cgaggagttc gacgcggtcc gtgaagcgct ggtcaacatc agcaccaagg gccgtggcga
      601 cgtgtccgag gcggagcgcg cccagattcg tgatgcggta gggaagacgg cggagaaacg
      661 cgttctgctg aagttcacgc cggagcgata cgtcagctgg gatcacgcca agctcggcgg
      721 gcgttactga ggcgcaggct tcaggccccc cgcggccgct gggcgaagag gtaggcgacc
      781 gccgccaatc ctgaagaccc gaaaatcatg cacatcacca cgatctggta gcgcacggca
      841 atcagcggat ccacgccgga caggatctgg cctgtcatca tccccggtag agacacgaga
      901 cccaccgcca gcagggcgtt gacctggggg atgagcgcgg catccagggc cgtcgtgcgt
      961 gcctcggcgt actcgacttc ccgggcctgc tcggactcga accgctcggc tgcgaggctc
     1021 accgtgttca tggcgttggc gaaaatcatg ccggccagcg gcaccacgaa gcgtggctcg
     1081 aaccagcggg gcaggtcgat gaccagctgg ctcaccaggg ccagcatggc caggccgcag
     1141 atggatatgg cggtcaggga ctgcagatac ttgcgcgcgc tgcggctctg taggggtcgc
     1201 agcgcgatcc aggcagcgac gcccatcatc atcacgagca cggcggccac gatccacggg
     1261 ttctcggtct cgaacacgaa cacgagcacg tagccgatcg ccaggagctg cacgagcatg
     1321 cggatgttgg caatcagcgc ctgcccggcc tggagggacc aggcgcgcat caccgccacg
     1381 agagccagac tcggcaggaa ggcgaccagc agggcgccga tggagacctc cgggaggccc
     1441 tgcactgtcg ctatgttccc gggcgctacg ggtcaggcgc ccagaacgaa gagccagatc
     1501 aggctgacca gggagcccat gaacatcagg gagcgcaggg tcgcccagcc gcctatgtaa
     1561 cagaccccat gcagcacccg gaacaccacg aaggccacgg ccagctgggc cgatctctcc
     1621 gggtccgccc ccaggacgtg ggcagtgagc gccgcagccg tgaacagggc cagggcctcc
     1681 caggcattct gctgcgcggc gtaggccctt gagccggcac ccgtcaacga cttgatctgc
     1741 tctcgggggt cgtcgttgtc cagcgtgccg agctgcttgt ttcggaagta accgccgacg
     1801 ccggccagaa cgatgggcat gagcatcacg atcagcaggc accaaagtgg ggtggtcatg
     1861 tctgtctcct agtacttgta cgattccggt ttgaatggac cgctttcgtc cacaccgatg
     1921 tattttgcct gctcggccgt cagtctggta accaccccgc cgaacccggc gaccatgagc
     1981 tcggcgacct cctcgtccag cttcttcggc agcacctcca cggtgatggg cgcgcgctgg
     2041 ttctccggct ggtcggccca gcgctgctcg tagaggtgca tctgagccag cacctgattg
     2101 gcgaaggagc cgtccatgac gcgcgacggg tggcccatgg cgttgcccag atttaccagg
     2161 cggccctcgg acagcaggat caggtagtcg tggcgatcgt cgcttcggaa aacctgatgc
     2221 acctggtcct tgaccatctc ccagcgccag tgctcacgca tgaaggcggt atcgatctcg
     2281 ttgtcgaagt gcccgatgtt gcagaccacg gcgcctgcct tgacggtgct gagcatgctg
     2341 gcatcgcaga cgtcggcgtt gccggtgcag gtgacgatga gatcggtgtc gtgcagcagg
     2401 cgggtatcga tgttctcgat gcggccggtg ttctcgccgc ccaggtaggg agaaaccacc
     2461 tcgaacccgt ccatgcaagc ctgcatggcg cagatgggat cgatctccac gacgcgcacg
     2521 atcattccct cctgacggag gctctgggcc gagcccttgc cgacgtcacc gtagccgatg
     2581 accagcgcgc gcctgccggc catcagcatg tcggtgccgc gcttgatggc gtcgttcagg
     2641 ctgtggcggc agccgtaacg gttgtcgttc ttggacttgg tgaccgaatc gttgacgttg
     2701 atggccggga ccttcagctc acccctatcg atcatctcgt agagccggtg cacgccggtg
     2761 gtggtctcct cgctgacgcc atggatggag tcgagcatct cggggtactt ctcgtggatc
     2821 acctcggtaa ggtcgccgcc atcgtcgaga agcaggttgg cgtcccacgg cttgccgtcc
     2881 ttgagaatcg tctgctcgat gcaccaccag tattcctcct ccgtctcgcc cttccaggca
     2941 aaaacgggta tgcccgccgc cgccatggcc gcggcggcat ggtcctgggt ggagaagatg
     3001 ttgcatgacg accaacggac ttctgcgccc agggcgacca gggtctcgat gagcaccgca
     3061 gtctgcaccg tcatgtgaat gcagccgatg accttcgcgc cggccagcgg ttgctggtcg
     3121 gcgtagcggt tgcgaagcgc catgagcgcc ggcatctcgg cctcggcgag gttgatctcg
     3181 cgcctgccga aatcggccag gctcatgtcg gccactttgt agtcagtcat ctgctctttc
     3241 cttttcgatg tgttgtctga aatggttcga tgggtttccg tatcccgggg ctacgtgccg
     3301 ttgcgggcgt agccgtggat ctgtatctga aagccgtttt tctgggccag gtactgggac
     3361 tcgaccggcg ccaggcccgc ctgggccgcc cactgctcca gctcgccggg ctcgaagccc
     3421 agccacaggt cgccgcacgc gtcggtgacc cattcctgct cgtggcgatc gagctccgcc
     3481 acgatgagca gaccgccgtg ccgcaggatc cgcgaagcgt ggcggaagaa tctctggggc
     3541 gacgccaggt gatgcacgac catggccgcg atcacggcgc ggtatcggcg aatcgccggt
     3601 agctcggtga aatccttctg gcgcagcttg acgttgggca gcccgtccag acgctcggcc
     3661 gcctgggcga gcatggtgga ggagctgtcg attccgatca ggtcgtcgaa gctcccggca
     3721 agcgtcgcaa gcagctgccc cccaccgggg ccgacctcga tgatcggccc ggaatcgacc
     3781 cctgcatggt cgagcagctc gacgatggct ggaacgtata cctgcgcttc gcagatgagc
     3841 gcctgctgat cggcaaaacg gtgtgcgttt tcggcaaaga agttgttgct gcgctggctt
     3901 cgctcggcgt gaatctccgc cgcccgctct gccagggcgg cacccagggg ggtgccgtcg
     3961 acggtttcga aaaggcgctg gcgcaatccg tcgtcgggtt ctgtgcaccg ccggtagtag
     4021 acgttgttgc cctctcgacg tttcgcgacc aggtttgccg tcagcaggac tttcaggtga
     4081 tggctgagcg ccggttgggg aatctgaaag aggtggcaca gctccgtgac gctgtaggat
     4141 ccgtcactga gcacccgcag gatgctcgcc cgcaggtgat cgcccacggc tttggcgacc
     4201 tcgacgacct cgtcgaggtt ggcttgctgg cctgccgtct ggcccatggg cggtggtgcc
     4261 tctacgcaac gagaggcgat cctatcagcg ccttcgcaca atatcaaaaa attttgatat
     4321 tgatggcgga aagatcgccg tgtcagaatg cggcgatgag ttggagctcg ccggatctcg
     4381 tcagcaagct
//
LOCUS       DBNHQR010000091         4012 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1718873, whole genome shotgun sequence.
ACCESSION   DBNHQR010000091 DBNHQR010000000
VERSION     DBNHQR010000091.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4012)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1718873"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..121
                     /locus_tag="AC16VR_02025"
     CDS             11..121
                     /locus_tag="AC16VR_02025"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6009959.1"
                     /translation="MGSADEVAEAVSYLVSDAAGYITGTTLAINGGLHMT"
     gene            261..494
                     /gene="acpP"
                     /locus_tag="AC16VR_02030"
     CDS             261..494
                     /gene="acpP"
                     /locus_tag="AC16VR_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR6009960.1"
                     /translation="MSSVEERVKKLICEQLGVKEDEVKDEASFVEDLGADSLDTVELV
                     MALEEEFETEIPDEEAEKITTVKEAIDYILAHQ"
     gene            581..1816
                     /gene="fabF"
                     /locus_tag="AC16VR_02035"
     CDS             581..1816
                     /gene="fabF"
                     /locus_tag="AC16VR_02035"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HIR6009961.1"
                     /translation="MTRRRVVVTGIGLLTPIGNDVDSAWSNALAGKSGAGPIEQFDAT
                     DFSVRICAPVKGFDATQYVDRKEVRRNDPFIVYGMAAGVQAIEDAGLDAHPADGHRYG
                     LAIGSGIGGLGTIEDTHGSLVAGGPRKVSPFFIPASVINMISGNLSIRYGFTGPNVAV
                     VTACTTGTHNIGFGARMIQSGDADVMVVGGAEYATTPVAVAAFSMMKALSTRNDEPEL
                     ASRPWDRDRDGFLLGEGAGVLVLEEYERAKARDAKIYAEVVGFGMSADAFHITSPAED
                     GAGAAACMQNALADAGLRSDQIDYINAHGTSTPLGDVAETVAIKRVFGDDTRVAVNST
                     KSMIGHLLGAAGSVEAIFTILAIRDQASPPTINLDNPGEGCDLDYVPNVARQMEIRAG
                     LSNSFGFGGTNGTLIFASV"
     gene            1846..2832
                     /gene="mltG"
                     /locus_tag="AC16VR_02040"
     CDS             1846..2832
                     /gene="mltG"
                     /locus_tag="AC16VR_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004422321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HIR6009962.1"
                     /translation="MRLFLVSFAALILLSGSAAFYVVRWNDAPLALTEPVVVVLEAGE
                     PFSKFAARISASGYLTHPRLWSRLARLRGLARQAKAGEYALQPGDTPERLLQRIVRGE
                     VVTYEVRLLEGWTVMQVLLALQENQVLEHELVGANVTTLLEALGLPGGHAEGLFYPDT
                     YQFVRGSSDRDILRRAYDRMQMVLEGAWDQRASGLPYRTPYEALIVASLVEKETGRDE
                     DREHISQVFVSRLNANMRLQTDPSVIYGLGERFDGNLKRVHLRESTPYNTYVIRGLPP
                     TPIALPGGKSIDAALHPGPGDYLYFVSRGDGTSQFSRSHAEHLAAVRKYQLK"
     gene            2829..3470
                     /gene="tmk"
                     /locus_tag="AC16VR_02045"
     CDS             2829..3470
                     /gene="tmk"
                     /locus_tag="AC16VR_02045"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HIR6009963.1"
                     /translation="MSVDSGSRGRFITLEGTEGVGKTSNLEHVCHLLQRAGYEVVATR
                     EPGGTPLAESIRSLLLEVREEPVDPTVELLMVFAARAQHVARVIEPALAAGRWVVCDR
                     FTDATFAYQGGGRGISMEDIATLESLVQGDLRPDLTLYLDVPVEVAAKRIAGRDHDRF
                     EREQMDFFERVRKTYLERAADEPQRVRVVDARQDLATVQAEIDRVVAEFLDRS"
     gene            3467..>4012
                     /locus_tag="AC16VR_02050"
     CDS             3467..>4012
                     /locus_tag="AC16VR_02050"
                     /inference="COORDINATES: protein motif:HMM:NF024575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009964.1"
                     /translation="MTALLPWHVTLADRIEGLMSAGRLPHGILITGTEGWGDSILAAW
                     LALRILAEDTDQNPRELAHPNLRWIVPDGAEIKVSAVRELGEFAFSTSQGGGAKVAVL
                     ENAHLLNRNAANALLKTLEEPPANTFLVLATSHPGRLLATIRSRCQRFVIQPDAQAAR
                     GWLQGRWSEAELASRLPETGGG"
BASE COUNT          746 a         1187 c         1336 g          743 t
ORIGIN      
        1 tgctggccgg atgggatctg cggacgaagt cgccgaagcc gtttcttatc ttgttagcga
       61 cgcggcgggc tatattacgg gaaccacgct tgccatcaac ggcgggctgc acatgacttg
      121 aaaagcgacg aaatttgagc tttcggattc cggattcaag cgattccggg tgcccgagcc
      181 tcaacttgaa ttggccaggg ggggtctata gaattcggcg ctccctgcac ccaggaacct
      241 gattgggaga tgactgcagc atgagcagtg tagaagaacg cgttaagaag ctgatttgtg
      301 agcagctggg agtcaaagag gacgaggtca aggacgaagc ttcgttcgtg gaggatctcg
      361 gtgcggattc tctggatacg gtcgagctgg tgatggcgct ggaagaagag ttcgagaccg
      421 agattcccga cgaagaagcc gagaagatca ccaccgtcaa agaggccatc gactatatcc
      481 tggcgcatca gtagccggcg aacccttgta attcacgtct aagccgcatc tacttgggat
      541 ggtgcggctt tttgtttctg ggtaatcgag agatcgaaac atgaccagac gacgcgtggt
      601 ggttaccggt attgggcttc tcacgccgat cggcaacgac gtcgactctg cgtggtcgaa
      661 cgcgctggcg ggcaagagcg gcgcagggcc catcgaacag tttgatgcca ccgacttcag
      721 cgtgaggatc tgcgcacccg tcaaagggtt cgacgcgacg cagtacgtag accgcaagga
      781 agtgcgccgc aacgatccgt tcatcgtcta cggcatggca gcgggcgtgc aggccatcga
      841 ggatgcgggg ctcgacgcgc atccggccga cggccaccgt tacggtttag ccatcggatc
      901 cggtatcgga ggtctgggga ccatcgagga cacacacggc agcctcgtcg ctggcggtcc
      961 gagaaaggtt tccccgtttt tcattcctgc cagcgtgatc aacatgatca gcggcaacct
     1021 gtccatacgc tacggattca ccgggcccaa cgtggcggtc gtgactgcct gcaccaccgg
     1081 aacccacaac atcgggttcg gggcgcggat gattcagagt ggcgatgccg acgtcatggt
     1141 ggtcgggggg gcggagtacg cgacgacccc cgtggcggtt gcggcattct cgatgatgaa
     1201 ggcgctctcc acgcggaacg acgagccgga gcttgccagc cggccctggg atcgtgatcg
     1261 ggacggattc ctgctcgggg aaggggcggg cgtccttgtc ctcgaggagt acgaacgggc
     1321 aaaggcccgg gacgccaaga tctatgccga ggtggttggg ttcgggatga gcgccgatgc
     1381 ctttcacatc accagccccg cggaggatgg cgcaggcgcc gctgcgtgca tgcagaacgc
     1441 gcttgcagac gcgggcctgc gttcggatca gatcgactac ataaacgccc acggaacatc
     1501 cacgccgctc ggcgacgtgg cggagacggt ggccatcaaa cgcgtcttcg gagacgacac
     1561 ccgggtggcc gtcaattcca ccaagtccat gatcgggcat ctgcttggcg cggcgggcag
     1621 cgtggaagcg atctttacga ttctcgccat ccgcgatcag gcgtctccgc cgaccatcaa
     1681 cctggacaat cctggcgaag gctgcgatct ggactacgtc ccgaacgtgg ccagacagat
     1741 ggagatcagg gctggtctga gcaattcgtt cggattcggc ggaaccaacg ggaccttgat
     1801 cttcgcatcg gtctgaacga cgcgcctgag gtagaatccg gcgcgatgag gctcttcctg
     1861 gtcagcttcg ccgccttgat ccttctctcc gggtccgcag cattctatgt ggttcggtgg
     1921 aatgatgccc cgctcgccct gaccgagccg gtggtggtcg tgctcgaggc gggcgaaccg
     1981 ttttccaagt tcgcagcccg gataagcgcg tcggggtacc tcacacaccc aaggctctgg
     2041 tcccggttgg ctcgtctgcg cggtcttgcc aggcaggcca aggccggaga gtacgcgttg
     2101 cagcctgggg atacgcccga acggctgctg cagcggatcg tccgcggtga ggtcgttact
     2161 tatgaagtcc ggctgctcga gggctggacc gtgatgcagg tgctgctcgc gttgcaggag
     2221 aaccaggtgc tcgagcacga actcgtcggc gcgaacgtca ccacgctgct ggaagccctg
     2281 ggactgccag gaggtcatgc ggaggggctc ttctatccgg acacctatca gttcgtgcgc
     2341 ggttcgtctg atcgggacat cctgcgcaga gcctacgacc ggatgcagat ggttctcgag
     2401 ggcgcctggg accagcgcgc gtcgggcctt ccctaccgaa ctccatacga agcgctcatc
     2461 gtggcatccc tcgtggagaa ggagacgggt cgcgacgagg atcgcgagca catcagccag
     2521 gtgttcgtca gccgactgaa tgccaacatg cgcctgcaga cggacccgtc tgtgatttac
     2581 ggtctgggcg agcggttcga cggaaacctg aaacgggttc atctgcgcga gtcgacccct
     2641 tacaacacat acgtgatcag ggggctcccg ccaacgccca ttgctcttcc cggtggcaaa
     2701 tcgatcgacg cggcgttgca tccgggcccc ggggattacc tgtatttcgt ctcccggggg
     2761 gacggtacca gccagttttc ccgaagccac gccgagcatc tcgccgcggt gcgtaagtat
     2821 cagctgaaat gagcgtcgac agcggcagca ggggtcggtt catcaccctc gaagggactg
     2881 agggcgtcgg caagacgagc aatctggagc acgtctgcca tcttctgcag cgcgccggat
     2941 acgaagtcgt cgcgacccgt gagcctggcg gcacgccgct ggccgagagc attcgatcgt
     3001 tgctgctcga ggtgcgcgag gagccggtag atcccaccgt ggagcttctg atggttttcg
     3061 ccgcccgcgc ccagcacgtc gcccgcgtca tcgaaccggc gctggcggcc gggcgctggg
     3121 tggtgtgcga ccggtttacg gacgccacct tcgcttatca gggcggcggg agaggcatct
     3181 cgatggaaga catcgccacc ctggaatccc tggttcaggg ggatctgcgt cctgatctga
     3241 cgctctatct cgacgtgccc gtcgaggtgg ctgccaagcg tattgccggg cgggatcatg
     3301 accgcttcga gcgcgagcag atggacttct tcgagcgcgt gaggaaaacc tacctcgagc
     3361 gggcagcgga cgaaccgcag cgcgtgcggg tcgtggatgc ccggcaggac ctcgcgacgg
     3421 tgcaggcgga aatcgaccgc gtcgtcgccg aattcctgga tcgctcatga cggcactact
     3481 gccctggcat gtgacgttgg ccgatcgtat cgagggcctc atgtctgccg gccggcttcc
     3541 gcacggcatc ctcatcaccg gcaccgaagg atggggcgac agtattctgg cggcctggct
     3601 ggcactgcgg attcttgcag aggataccga tcagaacccc cgggagctgg cgcatccgaa
     3661 tctgcgctgg atcgttcccg acggtgcgga gatcaaggtc agcgcagtga gagaactcgg
     3721 cgagttcgct ttttccacca gccagggcgg cggtgccaag gtcgccgttc tggaaaacgc
     3781 tcacctgttg aatcgcaacg cggcgaacgc gcttctgaag accctggagg aaccgccggc
     3841 caacacgttt ctcgtcctgg cgacgagcca tcctggacgg ttgctcgcga cgatccgcag
     3901 ccgttgtcag cggttcgtca tccagccgga tgcgcaggct gcccgtggct ggctgcaggg
     3961 tcggtggagc gaggccgagc tggcctcgcg gctgccggaa accggcggcg gg
//
LOCUS       DBNHQR010000092         3629 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_325746, whole genome shotgun sequence.
ACCESSION   DBNHQR010000092 DBNHQR010000000
VERSION     DBNHQR010000092.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_325746"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="AC16VR_02055"
     CDS             complement(<1..608)
                     /locus_tag="AC16VR_02055"
                     /inference="COORDINATES: protein motif:HMM:NF023165.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HIR6009965.1"
                     /translation="MITGFHCSTSWVRQRARVVALLGLLTLLVAGNTARGEPAMEAVA
                     ADGSTYPVEPVALLDPAALEELVGPIALYPDDLLAIALPAATYPLQVVQASRFLQSRE
                     ENPSVQPAEGWDDSVVALLNYPEVVELLNEDLDWTWKLGEAVLAQQEDVLEAVAAFRE
                     RARLAGNLRSDDFQLVESADEGIRIRPRDPEVIYVPYYEPSRV"
     gene            complement(782..1810)
                     /locus_tag="AC16VR_02060"
     CDS             complement(782..1810)
                     /locus_tag="AC16VR_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HIR6009966.1"
                     /translation="MCQSHDVANETENADPRSGYRLAEGWPHYPADMQFEMGSGVAVD
                     DAGVIYLFTRDIERWAAHPLAMKDKMGKSSISMFDRAGNYLGKWGPSDERGFALGAHT
                     LYIEKDGMFWTTDRDGHTVRKYHPDGTLLLTLGEFGQWGAGTDHFNGPTAVLVQENGN
                     IVVSDGYWNSRLVWFSPEGEFIREVGTWGTGPAEFGTPHAIAQDAQGRILVADLAGGN
                     FHPYMTLPGQIAEHRTRQIPDAKSRIQILDPDGAYIGEWDHIMPLSLAVYGDHVYASD
                     KMSNLAILDAATGKVEERVDNIAIYIHQLAMDAHGDIYTASVYPEHAGEKRGPEGPSH
                     RRWTRDGA"
     gene            complement(1853..3010)
                     /locus_tag="AC16VR_02065"
     CDS             complement(1853..3010)
                     /locus_tag="AC16VR_02065"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6009967.1"
                     /translation="MDDELDLMGPEEMADPAVSARRLLEGPRCRLYHGFDPPFYVVSR
                     YDDVSAIVRDPGRFLSGHGQGPNFVAPMGVVSDPPQHTFFRELVQPDFLPRSIALLRP
                     RLEAIADELLDAVAEETVWDVHDRLSFPLPVTIICEIFGIPTDDIEQFKIWSDASVAA
                     LSSQNPQQYDAELARMQAYVLERLRGTRRRPGDGSLISRIARARRDGVPVTDEEAVRL
                     VLQLFVAGNETTTSLITNFVWRMLAGDGLWADFCAGRVDVDRAINESLRFDPPLLGLF
                     RTTAVDVDVGGTVIPAHSKVLTHYGAANRDPAAFRDPDVFDVGRTGRKVLSFGLGIHV
                     CLGRELALLEARVALQGLARRFPRLELVDEGERIGPFLFWGRRHLPVRNRA"
     gene            complement(3003..>3629)
                     /locus_tag="AC16VR_02070"
     CDS             complement(3003..>3629)
                     /locus_tag="AC16VR_02070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MsnO8 family LLM class oxidoreductase"
                     /protein_id="HIR6009968.1"
                     /translation="LMELMLYLGDPVEGQRVHAVPGAGSKVPVWILGSSLFGAEVAAA
                     LGLPYAFASHFAPAAMDQAIEVYRKNFKPSEHLDAPYVMLGFNVCAADTEEEAVYLRS
                     SSLQAILQLRQGAPGRLPPPVHDFDATLTPEQRYLIDRFSLCSAVGTRETVERSINDF
                     LARTGADELIIVGQIFDHQARLRSFEIVAELIGDLQPGDSTRVAGQHG"
BASE COUNT          650 a         1179 c         1209 g          591 t
ORIGIN      
        1 accctggaag gttcgtagta gggaacgtag atcacctcgg gatcccgcgg ccggatccgg
       61 ataccctcgt ccgcgctctc caccagctgg aagtcgtcgc tgcgcaggtt gccggcaagc
      121 cgggcgcgtt cccgaaacgc cgcgaccgcc tcaagcacat cctcctgctg ggcgagcacg
      181 gcctcgccga gcttccaggt ccaatccaga tcttcgttca gcagctccac cacctcgggg
      241 tagttgagga gcgccaccac cgagtcgtcc cacccctcag ccggctgcac ggaagggttc
      301 tcctcgcgcg attgcagaaa ccgagaagcc tgcacgacct gcagcggata ggtcgcggcg
      361 ggcagggcaa tagccagaag gtcgtcggga tagagggcga tgggcccgac cagttcctcc
      421 agggccgccg gatccaggag cgctaccggc tccaccgggt aggtgcttcc gtccgcggcc
      481 acggcttcca tggccggctc cccacgggcc gtgttgcctg ccacgagcag ggtgagcaac
      541 ccgagcaagg cgaccacccg ggcccgctgc cggacccagg acgttgagca atggaaccct
      601 gtaatcatcc gatgcctctc agcttcctga tccgcgtggc gaggcagtcc ctggcgttct
      661 ttccactcat atcggacgag actatccccg tcaacctgaa cgggacatga agggcagccg
      721 gtcaaccagg gggcggggcg gacgggcggt ccgcgcagga aaggatcaga ttccttcggg
      781 atcaggcgcc gtcgcgggtc cagcgccggt gggacggacc ctcggggccg cgcttctcgc
      841 cggcgtgctc cgggtagacg gatgccgtgt agatatcacc gtgggcgtcc atcgccagct
      901 gatggatgta gatggcgatg ttgtcgacac gctcttccac cttgccggtg gccgcatcca
      961 gaatcgcgag gttgctcatc ttgtcgctgg cgtagacgtg atcgccgtag acggcgaggc
     1021 tcagcggcat gatgtgatcc cactcgccga tgtacgcgcc gtccgggtcg aggatctgaa
     1081 tacggctctt ggcgtcggga atctgccggg tccgatgctc ggcgatctgg ccgggcaggg
     1141 tcatatacgg atgaaagttg ccgccggcga ggtcggccac cagtatccga ccctgggcgt
     1201 cctgagcgat ggcgtgaggc gtgccgaact ccgccggccc agtgccccag gtgcccacct
     1261 cgcggatgaa ctcgccctcg ggcgaaaacc acaccagccg ggagttccag tagccgtccg
     1321 acaccacgat gttgccgttt tcctgcacga gcacggctgt ggggccgttg aaatggtcgg
     1381 tccccgcacc ccactggccg aactcgccga gggtcagcag cagggttcca tcgggatgat
     1441 atttgcgcac cgtgtggccg tcacggtccg tggtccagaa catgccgtcc ttttcgatgt
     1501 agagcgtgtg ggctccgagg gcgaagccac gctcatcgga ggggccccac ttgccgaggt
     1561 agtttccggc ccgatcgaac atggagatgc tgctcttgcc catcttgtcc ttcatggcca
     1621 ggggatgagc ggcccagcgc tcgatgtccc gggtgaacag atagatcacg ccggcgtcgt
     1681 ccaccgctac cccggagccc atctcgaact gcatgtcggc cgggtaatgg ggccaacctt
     1741 ccgccaggcg gtaaccgctg cgtggatcgg cgttttcggt ctcgttggct acgtcgtggg
     1801 attggcacat gacttcattg taggcgaggt ccaggcgctt agggaatggc cgtcaggctc
     1861 gattacggac cggcaggtgc ctgcgacccc agaagagaaa cgggccaatc cgttcgcctt
     1921 cgtccaccag ctcgaggcgc ggaaaccgcc gcgcgagacc ttgcagggcc acccgagcct
     1981 cgagcaaagc cagctcgcgg cccaggcaga cgtggatgcc gagcccaaag gaaagaacct
     2041 tccggccagt ccggcccacg tcgaatacgt ccggatcccg gaacgccgcc gggtcccgat
     2101 tggcagcgcc gtagtgggtg agcaccttgg agtgagcggg tatcacggtt cccccgacat
     2161 cgacgtccac ggccgtggtg cggaagagtc cgagaagggg cggatcgaat ctgaggctct
     2221 cgttgatcgc ccggtccacg tcgacgcggc ccgcgcagaa atcggcccaa agcccgtcgc
     2281 ctgccagcat gcgccagacg aaattggtga tgagcgaggt cgtggtctcg tttcccgcga
     2341 cgaagagctg cagcaccagg cgaacggctt cctcgtccgt caccggcacg ccgtctcggc
     2401 gggcacgggc gatgcgcgag atcaggctgc cgtcgccagg acggcgccgt gtcccgcgca
     2461 gccgttccag cacgtaggcc tgcatgcgcg caagctccgc gtcgtactgc tgcgggttct
     2521 gggaggacag agcggcgacg gaggcgtcag accagatctt gaactgctcg atgtcgtccg
     2581 tgggtatgcc gaatatctcg cagatgatgg tcaccggcag cggaaaggaa agccggtcgt
     2641 ggacgtccca gaccgtctct tccgcgaccg cgtcgagcag ctcgtcggcg atggcttcca
     2701 ggcgaggtcg tagcagggca atggatcttg gcaggaaatc aggctggacc agctcgcgga
     2761 aaaaggtgtg ctgaggcggg tcggacacca cgcccatggg cgcgacgaag ttgggaccct
     2821 gaccgtggcc gctcagaaaa cgccctggat ccctcacaat ggcgctcacg tcgtcgtagc
     2881 gggagacgac gtagaacggc gggtcgaacc cgtggtagag ccggcagcgc gggccttcga
     2941 gcagtcgccg ggcggagacg gccgggtcgg ccatctcctc gggccccatc aggtcgagct
     3001 cgtcatccat gctgcccggc aacccgcgtg gagtccccgg gctgtaagtc tccgatcagc
     3061 tccgcaacga tctcgaacga ccgcagccgg gcctggtgat cgaagatctg gccgacgatg
     3121 atgagctcgt cggcacccgt ccgggcgagg aagtcgttga tggaacgctc taccgtctcc
     3181 cgtgtgccca ccgccgagca cagggaaaac cgatcgatga ggtaacgctg ctccggcgtg
     3241 agggtggcgt cgaagtcgtg caccggcggc ggcagccgcc ccggcgcacc ctggcgcagc
     3301 tggaggattg cctgcaggga cgagctgcgc aggtaaacgg cttcctcctc cgtgtccgca
     3361 gcgcagacgt tgaaaccgag catgacgtaa ggcgcatcga gatgctccga gggcttgaag
     3421 ttcttccggt agacctcgat ggcctgatcc atggccgccg gggcgaagtg agaggcaaac
     3481 gcatacggca gcccgagggc tgcggcgacc tccgcaccga acaggctcga gcctaagatc
     3541 cataccggca ccttggaccc cgccccgggc accgcatgaa cgcgctgacc ctcgaccgga
     3601 tcgcccagat agagcatgag ctccatgag
//
LOCUS       DBNHQR010000093         3138 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_769612, whole genome shotgun sequence.
ACCESSION   DBNHQR010000093 DBNHQR010000000
VERSION     DBNHQR010000093.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_769612"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1781)
                     /gene="secD"
                     /locus_tag="AC16VR_02075"
     CDS             complement(<1..1781)
                     /gene="secD"
                     /locus_tag="AC16VR_02075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HIR6009969.1"
                     /translation="MANSSGVVGQGLLGLSKARRRAPNTHSVGRYLFILFVVVIGGVY
                     AAPNLFQSDPALQIKSTNEANPLNQEVLERVAAALAARGVTVKGTELAGDSALVRVGT
                     DEQQLRGRDIATAELNPESSGRYVVALNRASTTPQWLQDAGGKPMSLGLDLSGGVHFL
                     LQVDMEKFLGDRMRSNQEAIRDLLVDARIRYVGRDWLDGRQLSIPFQTAEGRDEASGL
                     IAETFDGFEIQERDVDGRPGLRLVLDDAKIGELEDLAISQNLQSLRNRVNELGVSEPL
                     VQRLGRSRIVLDLPGIQDSARAKEIINKFANLEFRLVAAPDARSSQTENYDYEGAPVS
                     LERRNIVTGDQVTNAVQEYDPESGMPQVSITLDNDGGRAMNEVTKDHVGDSMAILFKE
                     LKVRTRRVVEDGEEKLQPYTVEEKRLINVATIQSALGFRFRITGLGLGEARDLALLLR
                     AGALAAPMYIVEERTVGASLGEENIEKGRQSIVIGFLLVMVFMLVYYRWFGLAANVAL
                     TMNLVLIVAIMSVLGATLTLPGIAGIVLTVGMAVDANVLIFSRIREELTDRPPQQAIS
                     AGFDRALLTILDANITTFFVAIILFSVG"
     gene            complement(1815..2156)
                     /gene="yajC"
                     /locus_tag="AC16VR_02080"
     CDS             complement(1815..2156)
                     /gene="yajC"
                     /locus_tag="AC16VR_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008568065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HIR6009970.1"
                     /translation="MNLFETTAYAADAGGPPPDAGMINLLFLGGFILIFYFLLWRPQS
                     KRRKEHQALMSGLAKGDEVVTTGGIVGQVTRVDDDFVKLQVANNIELRVQKSSVGATL
                     PKGTLKSLDGD"
     gene            complement(2143..>3138)
                     /gene="tgt"
                     /locus_tag="AC16VR_02085"
     CDS             complement(2143..>3138)
                     /gene="tgt"
                     /locus_tag="AC16VR_02085"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003443399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HIR6009971.1"
                     /translation="MTPRALKEVGTQMLLGNTFHLMLRPGERLIESLGGIHEFMHWDG
                     PVLTDSGGFQVWSLGELRKISEEGVVFRSPVNGDRVELTPERSVAVQRALGSDVLMVF
                     DECTPYPATEAEARLSMELSMRWAERSLRAHQPSSGGLLFGIVQGGMFPALRQASLES
                     LVELDFPGYAIGGLSVGESKSEMREVLDRLLPLMPTTKPRYLMGVGTPEDLVRAVGLG
                     VDMFDCVMPTRNARNGYAFTSAGTLKIRNARHRESDLPLDERCGCYTCANFSRAYLHH
                     LDKCGEMLGSMLMTHHNLHYYHWLMAELRASIESGKFRTLIETLRTDWNSPDEPI"
BASE COUNT          584 a         1015 c          983 g          556 t
ORIGIN      
        1 ccgacggaaa agagaatgat ggccacgaag aaggtcgtga tgttcgcgtc gagaatcgtc
       61 agcagagccc tgtcgaagcc ggcagatatc gcctgctgag ggggacggtc cgtgagctct
      121 tcgcggatgc gggaaaagat cagcacgttg gcgtccaccg ccatgccgac cgtgagcacg
      181 atccccgcga tgcctgggag cgtcagagtg gcgccgagca ccgacatgat ggcaacgatc
      241 agaaccaggt tcatggtcag ggcaacgtta gccgccagcc cgaaccatcg gtagtagacc
      301 agcatgaaca ccatgaccag caggaagccg atgacgatgg actgacgccc cttctcgatg
      361 ttctcctcgc cgaggctcgc cccgacggtc cgctcctcga cgatgtacat gggagcggcc
      421 agggcgccgg ctcggagcag cagagcgaga tcgcgggcct cgcccagacc cagccccgtg
      481 atgcggaagc ggaatcccag cgcgctctgg atggtggcca cgttgatcag gcgtttctcc
      541 tccacggtgt agggttgcag cttttcctcg ccatcctcga ccacacgacg ggtgcggacc
      601 ttgagctcct tgaacagaat ggccatggag tccccgacat gatccttggt cacctcgttc
      661 atcgcccggc cgccgtcgtt gtcgagggtg atgctcacct gaggcatgcc ggactcgggg
      721 tcgtattcct gaaccgcgtt ggtcacctga tcaccagtga cgatgttgcg tcgctccagg
      781 gacacgggcg ccccttcgta atcgtaattt tcggtctggg aggaacgggc gtcgggcgcg
      841 gcgacgagac ggaactccag attggcgaac ttgttgatga tctccttcgc ccgggcgcta
      901 tcctggatac cgggcaggtc gagcacgata cgggaccgcc cgaggcgctg aaccagaggc
      961 tcggaaaccc ccagctcgtt gacccggttg cgcaggctct gcaggttctg ggatatggcg
     1021 aggtcttcca gctccccgat tttcgcatcg tcgagcacga gccgcaggcc cggccggcca
     1081 tccacgtcgc gctcctggat ctcaaagccg tcgaatgtct cggcgatcag cccgctggcc
     1141 tcgtccctgc cctctgcggt ctggaaagga atgctcagct gcctgccgtc gagccagtct
     1201 cgtcccacgt agcggatgcg ggcatctacc agcagatcgc ggatggcttc ctggttgctg
     1261 cgcatccggt cgccgaggaa cttctccatg tcgacctgaa gcaggaaatg cacgccgccc
     1321 gagagatcga ggcctagcga catgggcttg ccgccggcat cctgaagcca ctgcggggtg
     1381 gtcgaggcgc ggttcagggc aaccacgtag cggcccgacg actccgggtt gagctcggcg
     1441 gtggcgatgt cgcgcccgcg cagctgctgc tcgtccgtgc cgacccggac cagagcggaa
     1501 tcgccggcca gctcggtgcc ctttaccgtt acgccgcgcg ctgccagcgc cgccgccacc
     1561 cgctccagca cctcctggtt gagcgggttg gcttcgttgg tactcttgat ctgcagcgcg
     1621 ggatcggact gaaacaggtt gggcgccgcg tatacgccgc caatgaccac gacgaacagg
     1681 atgaacagat accgcccgac ggagtgagtg ttgggcgctc ggcgccgcgc cttcgacaac
     1741 ccgagcaggc cttgtcccac cacaccactc gaattagcca cggctggacg ttctccgcgc
     1801 ttgccagccg cgcactaatc gccgtccagg gatttcagcg tgcccttagg cagcgtcgcg
     1861 ccgacggagc tcttctgcac gcgcagctcg atgttgtttg ccacctggag cttgacgaaa
     1921 tcgtcatcca ccctggtcac ctgaccaacg atgccgcccg tggtgacaac ctcatcccct
     1981 ttggccaggc ccgacatcag cgcctgatgt tccttgcggc gcttgctctg agggcgccac
     2041 aggagaaagt agaagatgag gatgaaaccg ccgaggaaaa gcaggttgat catgccggca
     2101 tcgggcggcg gaccgccggc atcggccgcg taggcagtgg tctcaaatag gttcatccgg
     2161 ggagttccag tcggttcgaa gggtctcgat gagggtgcga aatttaccgc tttcgatgga
     2221 ggcgcgcaat tcagccatca gccagtggta gtagtggagg ttgtggtgcg tcatcagcat
     2281 gctgcccagc atctcgccgc atttgtccag atgatgaaga taggcccgcg agaaattcgc
     2341 gcaggtgtag cagccgcagc gctcatcgag gggcaggtcc gattcccgat gcctggcgtt
     2401 gcggatcttc agggttcccg ccgacgtgaa ggcgtagccg ttgcgcgcgt tgcgcgtggg
     2461 catcacgcaa tcgaacatgt ccacgccgag ccccactgca cgaacgaggt cctcaggcgt
     2521 gccgaccccc atgagatatc ttggtttggt cgtgggcatc agcggcaaca gccggtctag
     2581 aacctccctc atctccgact tgctctcccc caccgagagc ccgccgatgg cgtaaccggg
     2641 gaagtcgagc tcgaccagtg actccaagct cgcctgacgc agagccggaa acatgccgcc
     2701 ctggacgatc ccgaagagga gaccaccgga cgagggttgg tgcgcacgca ggctgcgctc
     2761 cgcccagcgc atcgacagct ccatggacag gcgggcctcg gcttccgtcg cggggtaagg
     2821 cgtgcactcg tcgaacacca tcaaaacgtc gctgcccaga gcgcgctgga cggcaaccga
     2881 gcgctcgggc gtaagctcga cacgatcgcc gttcaccggc gagcggaaaa ccacgccctc
     2941 ctcgctgatc ttgcgcagct ctcccaggct ccaaacctga aagccaccgc tgtcggtgag
     3001 gactggacca tcccagtgca tgaactcgtg aatgccgccc aaggattcaa tgagccgttc
     3061 tccggggcgc agcatcagat gaaaggtatt gccgagcagc atctgcgtcc ctacctcctt
     3121 cagcgcgcgc ggcgtcat
//
LOCUS       DBNHQR010000094         1094 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_515347, whole genome shotgun sequence.
ACCESSION   DBNHQR010000094 DBNHQR010000000
VERSION     DBNHQR010000094.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_515347"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..>1094)
                     /locus_tag="AC16VR_02090"
     CDS             complement(63..>1094)
                     /locus_tag="AC16VR_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR6009972.1"
                     /translation="VTLGLMSLPTMLRDRYDPAFASGTISATGTLGQIIPPSIALVLL
                     GEVLSNAYQQAQLNQGIFAPDTVSVGDLFAGAVVPGLILVGLYLAYTMLVAFLRPGTA
                     PAASSVADAVPDAVPDAVPDGSGWQLLGGLLPPMLLIVAVLGSILAGYATPTEAAGVG
                     AAGATILAAAYRALDVGVLRRVCRSTFDTTAMVFFILVGASLFSLVFRGYGGDDLVHS
                     WFNDMPGGTWGALAIVMLVIFLLGFMLDFIEITFVVVPIVGPVLLAMGVDPIWLGVLI
                     AVNLQTSFLTPPFGFALFYLRGVAPPTVSTADIYRGVAPFVLLQILLLCLLFIWPSLA
                     TWLPERIYR"
BASE COUNT          239 a          345 c          377 g          133 t
ORIGIN      
        1 ggcgtcgcaa agagcctccc cgtcccatac gcggctcacc gtaatggtgg ttggccgctc
       61 gctcaccggt agatgcgctc cggtaaccag gtcgcaaggc tgggccagat gaagagcagg
      121 cacaggagca ggatctgcag caacacgaac ggggccacgc cgcggtagat gtcggctgtg
      181 gaaaccgtgg ggggagccac gccgcgcaga tagaagagcg cgaagccgaa cggtggcgtc
      241 aggaacgagg tctggagatt gaccgcgatc agcaccccca gccagatcgg gtctacaccc
      301 atggccagaa gcacggggcc gacgatgggc accacgacga aggtgatttc gatgaagtcg
      361 agcatgaagc cgagcaggaa gatcaccagc atcacgatgg cgagcgctcc ccaggtgccg
      421 cccggcatat cgttgaacca gctgtgaact aggtcgtcgc cgccgtatcc cctgaagacc
      481 agagaaaaca aggaggcgcc gaccaggatg aagaaaacca tggccgtggt gtcaaacgtc
      541 gaccggcaca cccggcgcaa gacgccgacg tccagcgcgc gataagccgc cgccaggatg
      601 gtcgctccgg ctgcgccgac accggcggcc tccgtcgggg ttgcgtagcc cgcgaggatg
      661 gaccccagca cggcaacgat cagcagcatg ggaggaagca gcccgcccaa gagctgccac
      721 cctgagccgt ccgggaccgc gtccggaacc gcgtccggga ccgcgtccgc gaccgagctg
      781 gcggcgggcg cggtgcccgg ccgcaggaag gcgaccagca tggtgtaggc cagatagagc
      841 ccgaccagga tgagtccggg caccaccgcg cctgcaaaca ggtccccgac cgacaccgtg
      901 tccggggcga agatgccctg attcaactgg gcttgctgat aggcgttcga gagaacctcg
      961 cccagcagca ccagggcaat ggacggagga atgatctggc ccagcgttcc cgtcgcgctg
     1021 atggtgccgc tagcgaaggc cgggtcgtag cgatcgcgaa gcatggtggg tagagacatc
     1081 agcccgaggg tgac
//
LOCUS       DBNHQR010000095         5061 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_262289, whole genome shotgun sequence.
ACCESSION   DBNHQR010000095 DBNHQR010000000
VERSION     DBNHQR010000095.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5061)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_262289"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..2175
                     /gene="ppk1"
                     /locus_tag="AC16VR_02095"
     CDS             88..2175
                     /gene="ppk1"
                     /locus_tag="AC16VR_02095"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HIR6009973.1"
                     /translation="MSANPSTDFAGLANPELYINRELSLLEFHRRVLAQAQEERVPLL
                     ERLRYLCISSSVLDEFFEIRVAGLKQQEAYRATQRGPDNLSPSEQLRRIFETVRELVE
                     EQYRVLNEDLLPSLDDQGIRILDPDEWSSKQRQWLKRYFKRELAPIMSPIALDPAHPF
                     PQPLNKSLTFIVTLEGEDAFGRRSGKAIVQAPRALSRVVRLPESCAATPNEFVLLSSI
                     IEVHVGDLFPGMKSTGCFQFRVTRNSDLFVDMEEVDDLLRALEGELSSRRFGDAVRLE
                     VARDCPDPLEAFLTGQFHLKPEDVYRCSGPVNLMRLATVPDLVDRPELKFPGFTPGIP
                     DRLRNANDMFEVIRQGDVLLHHPFESFAPFIDFLRQAAADPQVLSIRQTLYRTGTESA
                     VVEALRDAATNGKEVLVVIELRARFDEEANIELANYLQRAGAQVVYGVVGHKTHAKMS
                     MVIRREGRPLRRYVHLGTGNYHTRTTRLYTDYGLFTCDPDIGDDVQQLFQQLTSMGKT
                     SGLKKLLQAPFTMHSSMLVFIEREAENARAGRPARILAKMNSLIEPQVIQALYAASQA
                     GVKIDLVVRGVCGLRPGIEGVSENVTVRSIIGRFLEHTRVFYFENEGDPRVYLSSADW
                     MNRNFFDRVEACFPIEDASLRDRIYRELGMYLGDNCQSWMLAADGKYRRLVSPEGERR
                     SAQEGLLEELAGD"
     gene            complement(2161..3687)
                     /locus_tag="AC16VR_02100"
     CDS             complement(2161..3687)
                     /locus_tag="AC16VR_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007161351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HIR6009974.1"
                     /translation="MTEATLNYGDVTQLAALDLGSNSFHLIVAQETNGRLQIIDKIKE
                     MVRLANGLDEKNELTGPATDTALACLERFGQRLNSLQRRNVRVVGTNTLRRARNAAAF
                     IERAEQALGHPVEIISGREEARLIYLGVSHSIEDNYERRLVIDIGGGSTELILGRHFE
                     AEKTESLHMGCVGISRQFFGDGRIRAGQFRAAVNHARKELVPVAGTYGRKSRDTVIGA
                     SGTILAAQAVLAEMDGSRDISLDGLERVREALIDAGHVDKLALPGLPPERAPVFPGGL
                     AVLIAAFKQLDIASMEATSGALREGLLYDLLGRVHHQDVRENSVADLCMRYHVDQTHA
                     RRVREMALALLAQVALEWDLTEPRHQLLLGWAADLHEIGMDIAHSQYHRHGGYLLANM
                     DLPGFSKGDQLELAALVRAHRRKFQQEDGDYPPHALRLAVLLRLAVLLHRNRANTALP
                     HVALRADGDALLLKFPRGWLKRHPLTRLDLDQEADYLSAVGVGLRIDENENAARSVAG
                     "
     gene            complement(3762..>5061)
                     /locus_tag="AC16VR_02105"
     CDS             complement(3762..>5061)
                     /locus_tag="AC16VR_02105"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HIR6009975.1"
                     /translation="AHGGNRRSGGNAAVPVMALGLGLLIIGYSGTFFGNLIKAAVSRQ
                     REYLADAAAVQFTRNPSGLANALKRIGGLPVGSTMSGSNAREISHMFFGQCSRPFFSG
                     LMSTHPPLEDRIRAIEPRWDGRLLERGQPSPTRPAGAAGFAGTGAAGIQAPGGISVGV
                     PAESIADEVGRLDQTSLDNAVGIIASIDPKLREAAHDPWSGRGLIYATLTHADGYARD
                     RQLAYLEENAEPGVPEHVFRLVPAVRHLDEPRKLALVEMAVPALKELSDPQFRRFIDN
                     VVAMIRSDRQIDLFEWVMHRLLIKELRPHFDGPERIRARYRSPDQVADAALTVLSALA
                     RSGHGDVGAQAKALATGAELLDLAPRMDTEDDPNFVRLGQALWQLRHLKPLVKPRVLK
                     ACAAVALADGQVSGREGALLQGIAATLDCPLPPSIYAPKS"
BASE COUNT          855 a         1624 c         1659 g          923 t
ORIGIN      
        1 cggcccccct tcggcaacac gaagctttaa cattggcctc cggcgcgcat ggtaaaatgc
       61 ggtgtcaatt gttagtcaga gtctcagttg agcgcaaacc cttccacgga cttcgccggg
      121 ctcgcgaatc cggagctcta catcaatcgt gagctctcgc tgctagagtt tcatcgccgc
      181 gtgctcgccc aggcccagga agagcgcgtg ccgctcttgg aacgcttgcg ctatctgtgc
      241 atatcgagct cggtgctcga cgagtttttc gagatccggg tcgccggtct caagcagcag
      301 gaggcgtatc gcgccacaca gcggggaccg gacaacctgt ctcccagcga gcagctgcgg
      361 cgcatcttcg agaccgtccg agagcttgtt gaagaacagt accgggttct caacgaagat
      421 ctgcttccga gtctcgacga tcagggcatc cgaattctgg atccggacga gtggagcagc
      481 aagcaaaggc agtggctcaa gcggtacttc aagcgggagc tcgcgccgat catgagcccc
      541 atcgcgctcg accccgcgca ccccttcccc cagccgctga acaagagcct caccttcatc
      601 gtcaccctcg aaggcgaaga cgccttcggc cgccgcagcg gcaaagccat cgttcaggca
      661 cctcgcgcgc tctccagggt ggtgcgcctg ccggagtcct gcgccgccac gccgaacgag
      721 ttcgttctgc tctcgtcgat catcgaggtg cacgtcggtg acctgttccc gggcatgaag
      781 agcacgggct gttttcagtt tcgcgtgacg cgcaacagcg atcttttcgt cgacatggaa
      841 gaagtcgacg acctgttgcg ggcgctcgaa ggcgagcttt cctcgcgccg gttcggggat
      901 gccgttcgcc tcgaagtcgc ccgggactgt cccgatcctc tggaagcttt tctcaccggc
      961 cagttccacc tcaagcccga agacgtctat cgctgcagtg gccccgtcaa cctgatgcgc
     1021 ctggcgaccg ttccggacct cgtcgatcgc ccggagctga agtttccggg gttcacgccg
     1081 ggtattcccg atcggctgcg taacgccaac gacatgttcg aggtcatccg tcagggagac
     1141 gtgctgctgc atcacccttt cgagtcgttc gcgcctttca tcgactttct gcgacaggcg
     1201 gccgccgatc cccaggtgct ttcgatccgt cagacgctct atcgcacggg gacggagtcg
     1261 gcggtggtgg aagcgctgcg ggacgcggcg accaacggca aggaagtgct ggtggtcatc
     1321 gagttgcgtg cgcgtttcga cgaggaggcg aacatcgagc tcgccaacta ccttcagcgc
     1381 gcgggcgcac aggtggttta cggcgtggtg gggcacaaga cccacgcgaa gatgagcatg
     1441 gtcatccgtc gggaaggccg tccgctgcgc cgctatgtgc atctgggcac cggcaactac
     1501 cacacgcgca ccacgcggct ttacaccgac tacggcctgt tcacctgcga ccccgacatc
     1561 ggcgacgacg ttcagcagct ctttcagcag ctcacgtcca tgggcaagac cagcgggctg
     1621 aagaagctgc tgcaggcacc gttcacgatg cattcgtcca tgctcgtctt catcgagcgc
     1681 gaagccgaga acgcccgcgc ggggcgaccg gcgcggatac tggccaagat gaactcgctc
     1741 atcgagccgc aggtcataca ggcgctgtac gccgcgagtc aggccggggt gaagatcgac
     1801 ctcgtggtcc gcggcgtatg cggactgcgc ccgggtatcg agggcgtgtc cgagaacgtc
     1861 accgttcgct cgatcatcgg gcggtttcta gagcacacca gggtgttcta ctttgagaac
     1921 gaaggcgatc cgagggtcta tctgtccagc gcggactgga tgaaccgtaa cttcttcgac
     1981 cgggttgagg cctgctttcc cattgaggac gcctccctgc gtgaccggat ctacagggag
     2041 ctcgggatgt atctcggcga caactgccag tcttggatgc tcgcagcgga cgggaagtac
     2101 cggcggcttg tcagcccgga gggcgagcgt cgaagcgctc aggaagggct gctcgaagag
     2161 ttagccggcg actgatcgcg cggcgttttc gttctcgtcg atgcgcagac cgactccgac
     2221 cgccgacagg tagtcggctt cctggtccag atccaggcgg gtgagcgggt ggcgtttgag
     2281 ccagccgcgg ggaaacttca gcagcagcgc gtctccgtct gctcgcagcg cgacgtgagg
     2341 gagcgccgtg ttggcgcggt tgcggtgcag gagcacggcc agtctcagca gcaccgctag
     2401 cctcagggca tgggggggat aatcgccgtc ttcctgctgg aacttgcgcc ggtgggctcg
     2461 gaccagcgcg gccagctcca gctggtcgcc tttggagaag cccggaaggt ccatgttggc
     2521 tagcaggtag ccgccgtgcc tgtggtactg gctgtgcgcg atgtccatgc cgatctcatg
     2581 cagatcggcc gcccacccca gcaggagctg atgtctcggc tcggtgagat cccattcgag
     2641 ggctacctgg gccaggaggg ccagcgccat ctccctcacc cggcgcgcgt gtgtctggtc
     2701 gacgtgatag cgcatgcaca ggtccgcgac gctgttctcc cgcacgtcct ggtggtgcac
     2761 ccgacccagc agatcgtaga gcagcccttc gcggagagcg ccgctggtgg cctccatgct
     2821 cgcgatgtcg agctgtttga aggcggcgat gagcacggca aggcctcccg ggaatactgg
     2881 cgcccgctcg gggggcagac cgggcaaagc cagcttgtcc acgtggccgg catcgatcag
     2941 ggcctcgcgc acgcgttcca gaccatcgag cgaaatgtcc ctgctcccgt ccatctctgc
     3001 aagcacggcc tgagcggcca ggatggtacc cgaggcgccg atcaccgtgt cccgggactt
     3061 tctgccgtag gttccggcca ccggaacgag ctcttttctg gcgtgattga cggccgctcg
     3121 aaactgtccg gcccggatac gtccgtcgcc gaagaactgt cgcgagatcc ccacgcatcc
     3181 catgtgcagg ctctcggttt tctccgcctc gaaatggcgg ccgaggatga gctccgtgct
     3241 gccgccgccg atgtcgatga ccaggcggcg ttcgtagttg tcctcgatgg agtgagatac
     3301 gcccagatag atcaggcggg cctcctcgcg cccggagatg atctccaccg ggtggcctag
     3361 agcctgctcg gcccgttcga tgaaggcggc cgcgttgcgc gcgcgcctca aggtattggt
     3421 ccccacgacg cgaacgttgc ggcgctgcag gctgttcagc cgctggccga accgctcgag
     3481 gcaggcgagc gctgtatccg tggcgggccc ggtcagctcg ttcttctcgt caaggccgtt
     3541 tgcgagcctg accatctcct tgatcttgtc gatgatctgc agccgcccgt tggtttcctg
     3601 agcgacgatg agatgaaagc tgtttgagcc caggtccagt gccgcgagct gggtgacgtc
     3661 gccgtagttg agagtggcct cggtcatggg gcccagtgcg gatcgaaagc gtcattctat
     3721 agagcaaggt cggacgcgca agcgggggcg cttgctccct ctcaggactt cggagcgtag
     3781 atcgagggcg gtaacgggca atccagcgtc gctgcgatgc cctgcagcag ggcgccctct
     3841 cggccgctta cctggccgtc ggcaagcgct acggcagcgc aggctttcag cacgcgcggc
     3901 ttcaccagcg gctttagatg gcgaagctgc caaagggcct gaccgagacg gacgaagttg
     3961 gggtcgtctt ctgtgtccat tcgaggggca aggtcgagca gctcggcgcc ggtcgccagg
     4021 gccttggcct gagccccgac atccccatga ccggaacgtg ccagcgcgga aagcaccgtc
     4081 agcgctgcat cggccacctg gtcgggggag cggtagcggg ccctgatccg ctccgggccg
     4141 tcgaagtgcg gacgcagctc cttgatcagc agcctgtgca tgacccactc gaacaggtcg
     4201 atttggcgat cggacctgat catcgcaacc acattgtcga tgaaccgcct gaactggggg
     4261 tcggacagct ctttcaatgc cggcaccgcc atttccacca gcgcgagctt gcggggctcg
     4321 tcgagatgcc tgaccgcggg aaccaggcga aagacgtgtt cgggcacgcc gggttccgcg
     4381 ttttcctcga gataggcgag ctgtcggtcg cgcgcatagc catcggcgtg ggtgagggtc
     4441 gcgtagatca gcccgcgacc ggaccagggg tcgtgagcag cttcgcgcag cttcggatcg
     4501 atgctcgcga tgatgcccac cgcgttgtcc aggctcgtct ggtccaggcg gccgacctcg
     4561 tccgcgatgc tctccgcggg gacccccacc gagatgcccc ccggtgcttg aatgcccgct
     4621 gcgccggtac ccgcaaatcc cgcggcgccg gcagggcgcg tcggggacgg ttggccacgt
     4681 tccaggagcc gaccgtccca gcgcggttcg atggcgcgga tccggtcttc gaggggggga
     4741 tgggtagaca tcagaccgct gaaaaacggc cgagagcact ggccgaagaa catgtggctg
     4801 atctcacggg cattcgatcc cgacatggtc gaccccacgg gcaggccgcc gatgcgcttg
     4861 agcgcattgg ccaggccgct ggggtttcgc gtgaactgca ccgccgcagc atctgccaga
     4921 tactcgcgct gtcggctcac cgcggccttg atgagattgc cgaagaaggt gcccgagtag
     4981 ccgatgatca gcagccccag gccgagggcc atcaccggca ccgccgcgtt gcccccggat
     5041 cggcggttgc cgccgtgggc c
//
LOCUS       DBNHQR010000096         1955 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1276210, whole genome shotgun sequence.
ACCESSION   DBNHQR010000096 DBNHQR010000000
VERSION     DBNHQR010000096.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1276210"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="AC16VR_02110"
     CDS             <1..701
                     /locus_tag="AC16VR_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6009976.1"
                     /translation="YVINGSKAFISGGGENEIYVCMLRTGDEGHRGISCIVVEKDTPG
                     LSFGAQERKLGWHSQPTAMVNFEDVRVPVSNRVGEEGEGFKIAMAGLDGGRLNIAACS
                     IGGAQRCLDEAIGYVKERRQFGSAIADFQNTQFMLADMATQLAAARALTHMAAQKVTE
                     NAADKTEFAAMAKLIATDSGSKIANDALQLHGGYGYLMDYPVERFVRDLRVHEILEGT
                     NQIMRMVIGRSLVS"
     gene            complement(760..1545)
                     /locus_tag="AC16VR_02115"
     CDS             complement(760..1545)
                     /locus_tag="AC16VR_02115"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6009977.1"
                     /translation="MAGSKTANEIFDLSEKVAIITGGSRGLGLNMAWAFAAAGARVIV
                     ASRKQEVCDAAAQEIQAGTGQECLGAAYHAGSWQDGDRLAELVYARFGRVDVLVNNAG
                     MSPLYRSLPDVSEALYDKVLDVNLRGPFRLSALIGARMAEGDGGSIINVSSTAAVQPT
                     PTELPYAMAKAGINAFTLGLARAYAPKVRVNCIMPGPFLTDISKAWDMDAFNERAKTT
                     IPLQRGGEPDEIVGAALYFASAASSYTTGSVLKVDGGSAYPAA"
     gene            complement(1636..>1955)
                     /locus_tag="AC16VR_02120"
     CDS             complement(1636..>1955)
                     /locus_tag="AC16VR_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6009978.1"
                     /translation="TGRAITARVAKELGMVNRVVPLEDLADETLDMAREIAEQPMMGL
                     KTAKESINQAQNEQGLFSALRSAMTLQQLSHANNNMVHGMGVVPEGARQVRDLIKKPP
                     LDE"
BASE COUNT          341 a          655 c          602 g          357 t
ORIGIN      
        1 actacgtcat caacggcagc aaggccttca tttcaggcgg tggcgagaac gaaatttacg
       61 tgtgcatgct gcgcacggga gacgagggac atcgcgggat ttcctgcatc gtcgtcgaga
      121 aggacacgcc cggcctgagc ttcggcgccc aggagcggaa gctcggctgg cattctcagc
      181 cgacggcgat ggtgaacttc gaggacgtgc gagtgcctgt ttccaaccgg gtgggcgaag
      241 agggcgaagg ctttaagatc gccatggccg ggctggacgg cggccggctc aacatcgccg
      301 cctgctcgat cggtggcgcg cagcgctgcc tcgacgaggc cattggctac gtgaaagaac
      361 gccggcagtt cggctccgcc atcgccgact ttcagaacac tcagttcatg ctcgccgaca
      421 tggccaccca gctggcggca gcacgtgcgc taacccacat ggccgctcag aaggtgacgg
      481 agaacgcggc cgacaagacc gagtttgccg cgatggcgaa gctgatcgcc acggattcgg
      541 gctccaagat cgccaacgac gccctgcagc tccacggcgg gtacggctac ctcatggact
      601 atccggtcga gcgcttcgtc cgagatctcc gcgtccatga gattctggaa ggcaccaacc
      661 agatcatgcg gatggtgatc ggacgatcgc tggtgagctg acggccgcca ggccccgctg
      721 cggcaatcgc caggtcccgc aaccaaggcc cgaccgcact caggctgcag ggtatgcgct
      781 gccaccgtcc accttgagta cggacccggt cgtgtagctg gaggcagccg aggcgaagta
      841 cagcgcagcc ccgacgatct catcgggctc cccgccgcgc tgcagcggaa tcgtcgtctt
      901 tgcgcgctcg ttgaacgcat ccatgtccca cgctttgctg atgtccgtca gaaagggccc
      961 cggcatgatg cagttgaccc gcaccttcgg cgcgtacgcc cgggcgaggc caagcgtgaa
     1021 cgcgttgatc cccgccttgg ccatggcgta gggcaattcg gtcggcgtcg gctggacagc
     1081 agcggtgctg ctgacgttga tgatcgatcc gccgtcgccc tcggccatgc gcgcgccgat
     1141 cagcgccgag agccggaaag ggcccctgag attcacgtcg agaaccttat cgtaaagcgc
     1201 ttcgctcacg tccggcaggg agcgatacag aggcgacatg ccggcattgt tgacgaggac
     1261 gtccacccgc ccgaaacggg catataccag ttctgccagc cggtcaccgt cctgccagga
     1321 gcccgcgtgg taggccgctc ccaggcactc ctgacccgtg ccggcctgga tctcctgcgc
     1381 tgccgcgtcg cagacctcct gctttcggct ggcaacgatc acccgggcac ccgccgcggc
     1441 gaaggcccag gccatgttca gacccagtcc ccggcttccg ccagtgatga tcgccacctt
     1501 ttccgaaagg tcgaaaatct cgttcgccgt tttcgatccg gccatggtca tcttgctcct
     1561 ctaacccgtt tccactgtgc cgccgtcact cgtcagcgcc aagctgacgg gttgccggct
     1621 cctgcgccgc cgcggtcatt catctaaagg cggtttcttg atgagatccc tcacctgccg
     1681 ggcaccctcg ggcaccacgc ccataccgtg caccatgttg ttgttggcat gggacagctg
     1741 ctggagagtc atggcgctgc gcagagcgct gaacaggcct tgttcgttct gagcctgatt
     1801 gatcgactcc ttggccgtct tgagccccat cattggctgc tcggctatct ccctggccat
     1861 gtcgagagtc tcgtcggcga gatcctcgag cggcaccacg cggttgacca tccccagttc
     1921 cttcgccacg cgcgctgtta tggcgcgccc ggtaa
//
LOCUS       DBNHQR010000097         3429 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_9224, whole genome shotgun sequence.
ACCESSION   DBNHQR010000097 DBNHQR010000000
VERSION     DBNHQR010000097.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_9224"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="AC16VR_02125"
     CDS             <1..1164
                     /locus_tag="AC16VR_02125"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR6009979.1"
                     /translation="FLQQEPLPDAAIEAAVAVMGSGRLHRYDAMADDPGETALLEMEF
                     ARYQGSRYALACASCGYALYVAMLSAGVGPGDGVLINGFTLAPVPGAVTNCGAQAIFV
                     DTADDLTVDLNDLERKAADASARFLLLSHMRGHIADMDAVTGICEENGLTLIEDCAHT
                     LGARWKGRLSGSFGAVSCFSTQTYKHMNSGEGGLLVTNRDDIMARAILYSGSYMLYDR
                     HLAGPPPEAFEGLSLTTPNYSGRMDNLRAATLRPQLARLDESCRRWNERYDVLAEGLA
                     ALPGVRLPERSPAESFVGSSIQFQLPGRTPAEMEDIVDRCAARGVALKWFGADEPRGY
                     TSRFDSWRYLGEPPELPGTRNALATLLDMRVPLTFDLEDCRHIVDIIADVIAG"
     gene            complement(1161..2084)
                     /locus_tag="AC16VR_02130"
     CDS             complement(1161..2084)
                     /locus_tag="AC16VR_02130"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6009980.1"
                     /translation="MKIGLATPDGLWASDLDSRRELLAQVAAAGVDHVFMADHVSFRN
                     GSGTDGFVEIAGLSQLHPTLGVMISIYLLPLRHPVPVARQLATVNRLAPGRVLFGVGI
                     GGEDRHEIEVCGVDPATRGRRMNESLSVLRGLMGGEEVTFHGEFFDLDRALIRPAIEP
                     AIPIIVGGRSDAALVRAGRLGDGWIGAWCSVRRFSQALGIIDDAAEQAGRVAVDWQHG
                     YQPWVGVGDTRDEARGRVAEAMEAFYKVPFESFEKYTPFGTPDEVASQLGGYVEAGCR
                     LMNFKVVAGNDAEAIAAMGEIGRALREVAAA"
     gene            complement(2104..>3429)
                     /locus_tag="AC16VR_02135"
     CDS             complement(2104..>3429)
                     /locus_tag="AC16VR_02135"
                     /EC_number="1.13.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF015043.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006720 - isoprenoid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HIR6009981.1"
                     /translation="GVYLRNTENPLHDAIGRYHPFDGDGMLHALFVDNGEAEYRNRFI
                     RTDGFAAELAEGRPLWAGLMESPKKSLRDGCGARTRLKDASSTDVVVHGGRAVTSFYQ
                     CGDLYEFDPRSLTQLGKADWVAGTTPGWGISAHTKVDEATGEMLFFNYAKEAPFMHYG
                     VVNAARELVHYVPVPLPGPRLPHDMAFTDNYAILNDFPLYWDPQALRAGAHAVRFFAD
                     QPSRFGIIPRRGGSHEVRWFEAKPTFVLHWINAYEDGDEVVLDGFFEENPNPKLTIDP
                     NDKRSMFRMLDMHVIGAKPHRWRFNLRTGATREEYLDDAVSEFGMINPAHGGKPYRYV
                     WAVTLKPGWFLMDGLVRLDVQTGEKQVYQLPDGVFLSESPMAPRVGSTAEDDGYVVTF
                     VTDMNRDASSAMIFDAADIEQGPVAQVQLPERICVGTHSCWSDKRNFAR"
BASE COUNT          580 a         1168 c         1132 g          549 t
ORIGIN      
        1 tttctgcagc aggaacccct gccggacgcc gccatcgaag cggccgtggc cgtcatgggc
       61 agcggccgcc tgcaccgcta cgacgccatg gccgacgatc ccggcgagac cgcgctgctg
      121 gagatggagt tcgcccgtta tcagggcagc cgctacgctc tggcctgcgc ctcctgcggg
      181 tacgccctgt acgtggcgat gctcagcgcc ggcgttggcc cgggagatgg tgtgctgatc
      241 aacggcttca ccctggcccc ggtcccggga gcggtaacca attgcggcgc ccaagccatc
      301 ttcgtggaca ccgcggatga tctgaccgtg gacctgaacg acctcgaacg aaaggccgca
      361 gatgccagtg cgcgcttcct cctgctatcc cacatgcgcg gccacatcgc cgacatggat
      421 gcggtgacgg ggatctgtga ggagaacggg ctcaccctca tcgaggactg tgcgcacacc
      481 ctgggcgcgc gctggaaggg ccgcctgagc ggcagcttcg gggccgtgag ctgtttcagc
      541 acccagacct acaagcacat gaactccggg gaaggcggcc tgctggtgac gaaccgggac
      601 gacatcatgg ccagggccat cctctactcc ggatcgtaca tgctctacga ccgtcatctc
      661 gccgggccgc cgccggaggc gttcgaaggc ctgtcgctga ccacgcccaa ctacagcggc
      721 cgcatggaca atctgcgcgc cgccaccctg cggccgcagc ttgcacggct cgacgagagc
      781 tgccggcgct ggaacgagcg ctacgacgtt ctcgccgaag ggctcgccgc gctccccggc
      841 gtccgactgc ccgagcgatc gcccgcggag tcctttgtcg gcagctcgat acagtttcag
      901 ctgcccggca gaacccctgc cgagatggag gacatcgtgg atcggtgcgc cgccaggggc
      961 gttgcgctga aatggttcgg cgccgacgag cccagggggt acaccagccg attcgacagc
     1021 tggcgctacc tcggcgagcc gccagagctg cctggaaccc gcaacgccct ggccacgctg
     1081 ctggacatgc gcgtgccgct caccttcgac ctggaggatt gccggcacat cgtcgacata
     1141 atcgccgacg tcatcgccgg ctaggctgcc gcgacctcac gcagggcgcg gccgatctct
     1201 cccatggcgg cgatggcttc cgcgtcgttg ccagccacca ccttgaagtt catgagccgg
     1261 catccggctt ccacgtaacc tcccagctgc gaggcgacct cgtcgggggt gccgaagggc
     1321 gtgtacttct cgaagctttc gaacggcacc ttatagaacg cttccatggc ctcggccact
     1381 cggccgcgcg cctcatcgcg ggtgtccccc acgcccaccc agggctggta gccgtgttgc
     1441 cagtccacgg cgacccgtcc ggcctgctca gccgcgtcgt cgatgatccc gagcgcctgg
     1501 gagaagcgtc tcaccgagca ccacgcaccg atccagccat cgccgaggcg accggcccgg
     1561 acgagagcgg catcggagcg gccgcccacg atgatgggaa tcgccggctc gatggcgggc
     1621 cgaatcagcg cccggtcgag atcgaagaac tcgccatgaa aggtcacctc ctcgccaccc
     1681 atgaggccgc gcagcacgct gaggctttcg ttcatgcgcc gcccccgtgt ggcaggatcg
     1741 acgccgcaaa cctcgatctc atgcctgtct tcacccccga tgccgacccc gaagagtacc
     1801 cggcccggcg ccagacgatt cacggtggcg agctggcgcg caacgggaac gggatgccgc
     1861 aggggcagca ggtaaatgct gatcatcacg cccagcgtcg gatggagctg cgaaaggccc
     1921 gcgatctcca cgaagccgtc ggtgcccgag ccgttgcgga aagacacgtg gtcggccatg
     1981 aagacgtggt cgaccccggc ggccgcgacc tgcgccagca gctcccggcg gctgtcgagg
     2041 tcggacgccc ataggccgtc aggcgtggca agaccgatct tcacgatggt tccggccgcc
     2101 cgttcagcgc gcgaagttgc gtttgtcgga ccagcacgag tgggtgccca cgcagatgcg
     2161 ctcgggcagc tggacctggg cgacgggacc ctgctcgatg tcggcggcat cgaagatcat
     2221 ggcgctggac gcgtctctgt tcatgtccgt gacaaaggtc accacgtagc cgtcgtcctc
     2281 ggccgtcgac ccaacccgcg gggccatggg gctttcgctg agaaaaacgc cgtccggcag
     2341 ctggtagacc tgtttctcgc cggtctgaac gtccagccgg acaaggccgt ccatgagaaa
     2401 ccagcccggc ttcagcgtga cggcccagac gtagcggtag ggtttacccc cgtgggccgg
     2461 gttgatcatg ccgaactcgg acaccgcgtc gtcgagatac tcctcccgcg tagcgccggt
     2521 gcgcaggttg aagcgccagc gatgcggctt ggcgccgatc acgtgcatgt ccagcatccg
     2581 gaacatcgag cgcttgtcgt tcgggtcgat ggtcagcttg ggattcgggt tctcctcgaa
     2641 gaagccgtcg aggaccacct cgtcgccgtc ctcgtaggcg ttgatccaat gcagcacgaa
     2701 cgtgggcttc gcctcgaacc agcgcacctc gtgggaacca ccgcggcgcg ggatgatgcc
     2761 gaagcggctc ggctgatcgg caaagaatcg cacggcgtga gcgcccgctc gcagcgcctg
     2821 cgggtcccag tagagaggga agtcgttgag gatcgcgtag ttgtcggtga acgccatgtc
     2881 gtgtggcagg cgcgggccgg gcaacggaac cggaacatag tgcaccagct cgcgggcggc
     2941 gttgaccacg ccgtaatgca tgaacggagc ttccttggcg tagttgaaaa agagcatctc
     3001 gcccgtggcc tcgtccacct tggtgtgggc cgatatgccc cagccaggcg tggtgccggc
     3061 cacccagtcg gccttgccaa gctgggtgag ggagcgggga tcgaactcgt acaggtcgcc
     3121 gcactggtag aagctggtga ccgcccggcc gccgtggacg accacatccg tgctggaagc
     3181 atccttgagg cgcgtgcgcg cgccgcagcc atcgcgcagg gatttcttgg gcgactccat
     3241 gagcccggcc cacaagggcc ggccttccgc cagctcggct gcgaacccgt cggtgcggat
     3301 gaaccggttg cggtattcgg cctccccgtt gtccacgaac agcgcgtgca gcatgccgtc
     3361 accgtcaaag gggtggtagc gcccgatggc gtcatgcagc gggttctccg tgttgcgcag
     3421 gtacacgcc
//
LOCUS       DBNHQR010000098         3097 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1212796, whole genome shotgun sequence.
ACCESSION   DBNHQR010000098 DBNHQR010000000
VERSION     DBNHQR010000098.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1212796"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="AC16VR_02140"
     CDS             <1..182
                     /locus_tag="AC16VR_02140"
                     /inference="COORDINATES: protein motif:HMM:NF016830.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HIR6009982.1"
                     /translation="NEDTYGTDLEDQYAGEIYFRWKIGKHFEITPNLQLLIDPALNPA
                     EDTIWVFGLRAKGSL"
     gene            204..1847
                     /locus_tag="AC16VR_02145"
     CDS             204..1847
                     /locus_tag="AC16VR_02145"
                     /inference="COORDINATES: protein motif:HMM:NF019042.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OprO/OprP family phosphate-selective porin"
                     /protein_id="HIR6009983.1"
                     /translation="MRNSIWIATVLLAWWPQWVSAESEGEGADLVLRNVILPDPGDAS
                     ADVVINVVIKDGKVLLVTKDEPPGGEGPVLDAAGTYLLGRLHTGSAPSFLLLNADPRE
                     EFSVLLDTATHAVFAISDGVVMKNDLPVPAASPDAPRRRSGWLAYTPPPISLPTSYRD
                     TSKWNRWETPFISGLFVGAVVLDRQFWQSQDAASESQVGDLTPFDGGEIRGLRFGAIG
                     TLNFPKPWVYTIFGATNAFDKGFNADDTDDVTLFDLRLDIPLFGSTLSLGKQKEPISM
                     ERTMGLVYLPIQERSAAMDGMLPARNTGLVLSGNAFAQRVSWAGGVFNDWLDTAGSFD
                     EGANQVVGRLTWLAASSADDSNLLHLGAGVRYSDAREGFRFSTEPEFNQAPLYIDTSI
                     DGTLLAAEDLLTWNLEASWRYGPLWLAGEYVKTDVDAPQYADPSFSGHHITASWALTG
                     EMRSYRRKNGTFSPLPVARSVHNNGPGAWELAARWSVFDANSGPISGGKTDIFSLGLN
                     WWLTPLFSVGANYRWITLDRDGLTGESDGFSTRILLMLE"
     gene            1887..3083
                     /locus_tag="AC16VR_02150"
     CDS             1887..3083
                     /locus_tag="AC16VR_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009984.1"
                     /translation="MAILSKLMSRVAILGLLAAPAAQAETWRPVSDKDELASLFSDTT
                     MTTTLRDGVTATAEYRADGTGTVRAWGDAFERRWSVSADSEVCIEAGDQTFCYTVETD
                     ADATDRYRSTNLETGERLILMIARQGGADPEPTQGTDSGAAGKPSAEEVAAKLANPNT
                     PLASLTTKLQYRTFDGNLADAGGQDGTTLLLQPSFPFALANGDLILFRPAIPILLDQP
                     VPGDDGFGSKSGLGDIAFDLAYARTTDSGMLIAAGVISSLPTATKKALGTDRWTLGPE
                     LLIGKITKKHVLGLFPNHQWDVAGSGDVDVSLTTIQAFYTHLPGGGWSVGTAPIMSYD
                     HIANQWTLPINLTVGKTVIWNGRPWKLSAEINYYVDQSDAFGPEWMIGINVAPVVENV
                     LAKWFR"
BASE COUNT          595 a         1014 c          983 g          505 t
ORIGIN      
        1 caaacgagga cacctacggc acggatctcg aggaccagta cgcgggcgag atctacttcc
       61 gctggaagat cggcaagcat ttcgaaatca ctccgaacct gcagctcctg atcgaccccg
      121 ccctgaaccc ggcggaagac accatctggg ttttcggcct gcgggcgaag gggtcgctgt
      181 agccgggagg cttagggggg gatatgcgca actcgatctg gatcgccacg gtgctgctcg
      241 cctggtggcc ccaatgggtc agcgcggaga gcgaaggaga aggcgcggac ctcgtgctgc
      301 gaaacgtgat tctgcccgac ccgggcgacg catcggcaga cgtggtgatc aacgtcgtga
      361 tcaaagacgg aaaggtcctt ctcgtcacca aggatgagcc ccccggcggg gaaggcccgg
      421 tgctcgacgc cgccggcacc tatctgcttg gcaggctgca cacgggcagc gcgccgagct
      481 ttctcctgct gaacgccgat cccagagagg aattcagcgt gctgctggac accgccaccc
      541 atgccgtttt cgccatcagc gacggggtcg tcatgaagaa cgacctgccg gtcccggcag
      601 catcgcccga cgcgcctcga cggcgctccg gttggctcgc ctacacacca ccgcccatct
      661 cgctgccgac gagctaccgg gataccagca agtggaaccg gtgggaaacg cccttcatct
      721 cgggcctgtt cgtgggcgcc gtggttctcg atcgccagtt ctggcagtcc caggatgcgg
      781 ccagcgaaag ccaggtcggc gatctgacac cctttgacgg cggcgagatc cgcggcctgc
      841 ggttcggcgc catcggcacg ctgaacttcc ccaaaccgtg ggtatacacg atcttcggcg
      901 ccaccaacgc gttcgacaaa ggtttcaacg ccgacgatac cgacgacgtg accctgttcg
      961 acctgaggct ggacattccg ctgttcggca gcacgttgag tctcggcaag cagaaagagc
     1021 ccatctccat ggagcgaacc atggggctcg tctacctgcc cattcaggaa cgttccgccg
     1081 ccatggacgg catgctgccg gctcgcaaca cggggctggt gctgagtggc aacgccttcg
     1141 cccagcgcgt cagctgggcg gggggcgtat tcaacgactg gttggacacc gcgggctcgt
     1201 tcgacgaggg agcgaaccag gtggtcggcc ggctgacctg gctggcggcc tcttccgccg
     1261 atgacagcaa cctccttcac ctgggcgcag gcgtgcgcta cagcgacgcc agggaaggat
     1321 ttcgcttctc caccgagccg gagttcaacc aggcgcccct gtacatcgat acgagcatcg
     1381 acggaactct actggccgcg gaggacctgc tgacctggaa tctggaagca tcctggcggt
     1441 acggccctct gtggctggcc ggcgagtacg tcaaaaccga cgtggacgcg ccgcaatacg
     1501 ccgatcccag tttctccggg catcacatca ccgcatcctg ggctctgacc ggcgagatgc
     1561 gctcgtatcg gcgcaagaac ggcacgttca gcccgctgcc ggtcgcccgg tccgtccaca
     1621 acaacgggcc gggagcttgg gagctggccg cgcgctggtc ggtgttcgac gccaactccg
     1681 ggccaatcag cgggggcaag acggacatct tcagcctggg cctgaactgg tggctgacac
     1741 cgctcttcag cgtcggtgcc aattaccgct ggatcaccct ggacagggac gggctgaccg
     1801 gcgagagcga cgggttctcg acgcgcatcc tgctgatgct ggagtaatcg gggcccgaga
     1861 gagagacaat gcggagatca gacaacatgg caatcctgag caagctcatg tcgcgcgtcg
     1921 cgatcctggg ccttctcgcg gcgcccgctg cgcaggcgga gacctggcgc ccggtcagcg
     1981 ataaggacga gctcgccagc ctcttcagcg acaccaccat gaccacgacg ctgcgcgacg
     2041 gcgtcaccgc caccgcggag tatcgtgcgg acggcaccgg aaccgttcgg gcctggggcg
     2101 acgcttttga gagaagatgg tcggtcagcg ccgatagcga ggtttgcatt gaggcgggcg
     2161 atcagacttt ctgctacacg gtggagaccg acgccgacgc caccgatcga tatcgatcaa
     2221 caaacctgga gaccggcgag cggctcatcc tgatgattgc ccgccagggc ggtgcggatc
     2281 cggagccaac ccaagggaca gactccggag cggcgggaaa gccgtcggcc gaggaggtcg
     2341 ccgcgaagct cgccaaccca aacacgccgc ttgcgagcct gaccaccaag ctgcagtacc
     2401 gcaccttcga cgggaacctc gcggacgccg ggggtcagga tggcaccacc cttctgcttc
     2461 aaccgtcctt tccgttcgcg ctggccaacg gggatctgat tctctttcgt cccgccatac
     2521 ccattctcct ggaccagccg gttccggggg acgacgggtt cggctccaaa tccggtcttg
     2581 gcgacatcgc cttcgacctg gcgtatgcca ggacgacgga cagcggcatg ctcatcgccg
     2641 cgggcgtgat ctcgtccctg ccaacagcca cgaagaaggc actgggcacc gaccgctgga
     2701 ccctgggtcc cgagctgctg atcggaaaga tcaccaagaa gcacgtcctg gggctgttcc
     2761 ccaaccatca atgggacgtt gccgggtccg gagacgtgga cgtcagcctt accaccatcc
     2821 aggctttcta cacccatcta cccggcggcg gatggagcgt gggcacggcg cccatcatga
     2881 gctacgacca tatcgccaat cagtggacgc tgccgatcaa cctcaccgtc gggaagacgg
     2941 tcatctggaa cgggcggccg tggaagctga gcgccgagat caactactac gtcgatcaga
     3001 gcgacgcgtt tggaccggaa tggatgatcg gcatcaacgt agcacccgtg gtcgagaacg
     3061 tgctcgcgaa gtggtttcgc tagccaacac agcaacc
//
LOCUS       DBNHQR010000099         1818 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1529027, whole genome shotgun sequence.
ACCESSION   DBNHQR010000099 DBNHQR010000000
VERSION     DBNHQR010000099.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1529027"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            333..965
                     /locus_tag="AC16VR_02155"
     CDS             333..965
                     /locus_tag="AC16VR_02155"
                     /inference="COORDINATES: protein motif:HMM:NF017269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HIR6009985.1"
                     /translation="MRAPALGLGLSLCVAAAPGMAGHSDGFPQAAPAGSHTHYTYADV
                     VGVEPLLEYRTVTEPRRDCRVAGYAAASSGTRSHRYTGPGHPDAHDHYQDHHDHGSPV
                     AAVIGGVVGGLLGSQFGSGSGKKAMTVAGALAGASVASSVSRSRSRHAGYTPRRCTTV
                     MTSREVEEVAGYRVRYVYQGEEFTKVVASDPGDRVRVQVQVTPAVGEPDW"
     gene            1028..>1818
                     /locus_tag="AC16VR_02160"
     CDS             1028..>1818
                     /locus_tag="AC16VR_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009986.1"
                     /translation="MDTNPRKGGSPLGRVLLPFLLLAAATASAVPDSGPVVRLFPTTV
                     LEDIRETGQVAKEMENSLQEVINRLDLQHQLYVESLCEGSDGDQGCERMAKQLGATYL
                     EMLTIMGDRLPEMELAVNNTRASLEKRLREELGHRTTPTSLQNALLGTTPASSAAAEQ
                     PALRGRSGVRLSDRFAQYYALVATQREGRGRSLAVVAADIYLDMEEASRLIAATQEEI
                     GRAALMEQLNQSFGLITPKMTEVVDGVKGILFGEASTDSPIAAPP"
BASE COUNT          308 a          601 c          604 g          305 t
ORIGIN      
        1 agtagtgatg ctcggacggc tcgacgattc cgatgtcgag gtcgtgccgt ccctgtctgc
       61 gcttgaggct ggcggcaacc gtaattcctg ccgccccgcc gccgatgatc aggatctgat
      121 gatggtctga cttggtcatg ttggctctcc ggccgggtcc tgcgagcggt gccgctggag
      181 tctggatgag catacgccgc tccggggggc tggagaagcg ggtgcgcccg gtagggcaaa
      241 cccgccccgg tcgggccgcc ccgttcagct tctgtttagc tgagctccca taacattcga
      301 gtctcgacag atttgggagt tccggaggaa acatgcgcgc accagccctt ggcctcggct
      361 tgagtctctg cgttgccgcg gcacccggca tggcgggcca cagcgacggt tttccgcaag
      421 ccgcccccgc cggatcccac actcactaca cctacgccga cgtcgtcggc gtggagcccc
      481 tgttggaata tcgcacggtc accgagcccc gaagggattg tcgggttgcg gggtacgccg
      541 cggctagttc cggcacccgt tcgcaccgat acaccggacc tggccatccg gacgcccacg
      601 accactacca ggatcaccac gatcacggca gtccggttgc cgccgtgatc ggcggcgtcg
      661 tgggcgggct gctgggcagc cagttcggca gcggcagcgg caagaaggcg atgaccgttg
      721 ccggagcgct ggcgggggcc tcggttgcca gcagcgtcag ccggtcccgg tcgcgccatg
      781 ccggctacac gccgcggcgc tgcacgacgg tcatgacgtc ccgcgaggtg gaagaggtcg
      841 ccggctaccg ggtgcgctac gtctatcagg gagaagagtt caccaaggtg gtggcttcag
      901 acccgggcga tcgcgtgcgc gttcaggttc aggtcactcc cgccgtcggg gagccagatt
      961 ggtgagcgct ctcttccgtc cagacacctg cgccgttttg cggcgcgcaa gcaaagagga
     1021 tatgcccatg gataccaacc caagaaaggg cggcagcccg ctcggccgcg tgctgctgcc
     1081 gttcctgctg cttgccgccg ccacggcttc cgccgtcccg gattcgggcc cggtcgttcg
     1141 gctcttcccg acaaccgtgc tcgaggacat acgtgagaca ggccaggttg ccaaagaaat
     1201 ggagaacagc ctgcaggagg tcatcaaccg gctcgatctg cagcatcagc tatacgtgga
     1261 gagcctctgc gagggctcag acggcgacca gggctgcgag cgcatggcca aacagcttgg
     1321 cgcgacctat ctggagatgc tcaccatcat gggtgaccgg cttccggaga tggaactggc
     1381 cgtaaacaat acccgcgcga gcctggaaaa gcgcctgcgg gaagagctcg ggcatcgaac
     1441 cacccccaca tcgctgcaga acgccctgct ggggactacg cctgcaagca gcgccgccgc
     1501 cgagcagccg gcgcttcgcg gccgttccgg ggtgcggctt tccgacaggt ttgcacagta
     1561 ctacgccctg gtcgcgaccc agcgtgaggg ccgtggccgc tcgctggcgg tggtcgccgc
     1621 cgacatctat ctggacatgg aagaagcttc ccgcctcatc gccgcgaccc aggaggaaat
     1681 cggacgcgcg gcgctgatgg agcagctcaa ccagtcgttc gggctgatca cgccgaagat
     1741 gaccgaagtg gtcgacggcg tgaagggcat cctgttcggc gaggcttcca ccgacagccc
     1801 cattgcggcg ccccccta
//
LOCUS       DBNHQR010000100         2934 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1402889, whole genome shotgun sequence.
ACCESSION   DBNHQR010000100 DBNHQR010000000
VERSION     DBNHQR010000100.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1402889"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="AC16VR_02165"
     CDS             <1..353
                     /locus_tag="AC16VR_02165"
                     /inference="COORDINATES: protein motif:HMM:NF013706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR6009987.1"
                     /translation="FNVTQGKNFERSGSVGPWLVSLDEIERFDDLHLTTKVNGELRQD
                     DRTDNLLFSFEYLISYLSTFMRLQPGDLISTGTPTGAGARFDPPRYLKPGDVVEVAVD
                     GIGTLRNTVEDEVA"
     gene            477..887
                     /locus_tag="AC16VR_02170"
     CDS             477..887
                     /locus_tag="AC16VR_02170"
                     /inference="COORDINATES: protein motif:HMM:NF016701.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR6009988.1"
                     /translation="MTVEGGCYCGAVRYKAEGDPVFSAQCHCRECQYLTGGAPNMFMA
                     VPRSGFEYTKGEPSAFTRTDIENPVTREFCGNCGTHLLTRANAMPDAVILKVGSMDDP
                     GQYPGPQVAIFCMDKQTFHTVPAGVPEFERLPGG"
     gene            complement(937..1416)
                     /locus_tag="AC16VR_02175"
     CDS             complement(937..1416)
                     /locus_tag="AC16VR_02175"
                     /inference="COORDINATES: protein motif:HMM:NF024375.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6009989.1"
                     /translation="MALQNIKPINDQLADIIVRADEPWIEQEPGMAWIKVLWTGAETG
                     RWVALFRWNKGYTAAPHKHLSDAHTYVLKGKLQVRDGVLNAGDYDYEPNGVLHGATTA
                     LEDTEYLFVCEGPIVFFDENGLTNYLSWEELARMRDAAKAGGDASMGISVTAESSAG"
     gene            complement(1544..2362)
                     /locus_tag="AC16VR_02180"
     CDS             complement(1544..2362)
                     /locus_tag="AC16VR_02180"
                     /inference="COORDINATES: protein motif:HMM:NF014899.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DODA-type extradiol aromatic ring-opening family
                     dioxygenase"
                     /protein_id="HIR6009990.1"
                     /translation="MGVVFAGVVSHAPGITGRAERADPALRDALYENFYRLGRELKES
                     RPDALIVIAAEHFANFFMDNMPAYCMGMADYYEGPIEDEQWLGISRVRIPGNAALAGS
                     LIESMLAEFDLAYAQEWKFDHGVMVPLHFLTPEYDIPVIPVNVNCQGPPLTPLARAYD
                     FGRALRRACDARPERIAIIGTGGISHWPATPDSGKINEAWDRAFLESWLANDRAALTS
                     FTDEETYLEAGQGGFEIRTFIAVSGATQGCAAELYFYEPIPIFAVGCTVARVHV"
     gene            complement(2362..2676)
                     /locus_tag="AC16VR_02185"
     CDS             complement(2362..2676)
                     /locus_tag="AC16VR_02185"
                     /inference="COORDINATES: protein motif:HMM:NF019366.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009991.1"
                     /translation="MSLYYVQKLLYQLNRDPHARRRYEEDFDELLAEYDLDAEELHAI
                     REPDIGLLYVMGVNGQILMHYAALRGYAWDEYIKAMRDGLAEHGDVRAGLYVTTDGRG
                     AV"
     gene            complement(2684..>2934)
                     /locus_tag="AC16VR_02190"
     CDS             complement(2684..>2934)
                     /locus_tag="AC16VR_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010324201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR6009992.1"
                     /translation="FGLAGYVWTGDVGRSHRLARDLDAGMIWVNSENNRHLPTPFGGM
                     KASGIGRDGGDYSFDFYMETKNVCISLGKHRVPSLGKG"
BASE COUNT          539 a          960 c          905 g          530 t
ORIGIN      
        1 agttcaacgt tactcaggga aagaatttcg aacgctccgg atccgtcggc ccgtggctcg
       61 tctccctcga cgagatcgaa cggttcgacg atctgcacct gaccaccaag gtcaacggcg
      121 agctacgcca ggacgaccgc accgacaacc tgctcttctc cttcgagtat ctgatcagct
      181 acctgtcaac gttcatgcgg ctgcagccgg gcgacctgat ctccacggga accccaacag
      241 gcgcaggagc gagattcgac ccgccacgct atctaaagcc cggtgacgtg gtcgaggtag
      301 cggtggacgg catcggcacg ctgagaaaca ccgtggagga tgaggtagcg tagtttcagc
      361 cctcggcgtc cgcagaggcc cagaggtctc ggccctgcca ggctggtgga ggaggcactt
      421 atccgccggg cgcgttacac tcgtaaggga agccaatcaa tcacaggaga ggtcccatga
      481 cggtggaagg cggatgttac tgcggagccg tgcgctacaa agcggagggc gacccggtct
      541 tcagcgcgca atgccattgc cgtgagtgcc agtacctcac cggcggcgcg ccaaacatgt
      601 tcatggccgt tcccaggtcc ggattcgagt acaccaaggg cgaaccgtcg gcgtttaccc
      661 gcacggacat cgaaaatccc gtcacccggg agttctgcgg caactgcgga acccacctgc
      721 tgacgcgggc caacgcgatg ccggatgcgg tgatcctgaa ggtcggctcc atggatgacc
      781 ccggccagta tccggggcct caggtggcca tcttctgcat ggacaagcag acgttccaca
      841 ccgttcctgc cggggtgcct gagttcgaac ggctgcccgg gggctgatcc aggccggagc
      901 gcccgcgccg ctttacggcg cgggcacagc agagattcag cctgccgagg actcagccgt
      961 gacggagata cccatggaag cgtcgccgcc ggccttggcc gcatcgcgca tgcgggccag
     1021 ctcttcccag ctcaggtagt tcgtcaggcc gttttcgtcg aagaagacaa ttgggccttc
     1081 gcagacaaac aggtactccg tgtcctccag ggccgtggtc gcgccgtgca gaacgccgtt
     1141 gggctcgtag tcgtagtcgc ccgcgttcag caccccgtca cgcacctgca gcttgccctt
     1201 caacacgtac gtatgcgcgt cggacagatg cttgtgaggc gctgcggtgt agcccttgtt
     1261 ccagcggaac agagccaccc atcggccggt ctcggcccct gtccagagca cctttatcca
     1321 ggccatgccg ggctcctgtt cgatccaggg ctcgtccgcc cggacgatga tgtcggcaag
     1381 ctgatcgttg atgggtttga tgttctgcag agccatggcc cctcctggtt atccgtcgtg
     1441 acaagttcgc cagccagcct acacccattg ggaaggcgtg ctcaatctat ttcccgtcat
     1501 cccgcctatg gccacacggg gagcgcgaag cggcagcagg tcgtcacacg tgaacccttg
     1561 ctacggtgca gcccaccgcg aatatcggga tcggctcgta gaagtagagt tccgccgcac
     1621 atccttgtgt ggctcccgaa acggcgatga aggtgcggat ctcgaacccg ccctgcccgg
     1681 cctcgagata ggtctcctcg tccgtgaaag aggtgagcgc cgcccggtca ttggccagcc
     1741 agctttccag gaaggcgcga tcccaagctt cgttgatctt tcccgagtca ggcgtcgccg
     1801 gccagtgcga gatgccgccg gtgccgatga tggcgatgcg ttccgggcgc gcatcgcaag
     1861 cccgcctcaa agcacgtccg aaatcgtatg cccgcgccag cggcgtcagc ggcggcccct
     1921 gacagttgac gttcacggga atcaccggaa tgtcgtactc cggggtgaga aagtgcagcg
     1981 ggaccatgac gccgtgatcg aacttccact cctgagcgta ggccagatcg aactccgcca
     2041 gcatggattc gatcaggctg ccagccagcg cggcgttgcc cggaatccgg acccgactga
     2101 tgcccagcca ctgctcgtct tcgatgggcc cttcgtagta atcggccatt cccatgcagt
     2161 aggcgggcat gttgtccatg aagaaattgg cgaagtgctc ggcggcgatg acgatcagcg
     2221 catccgggcg cgactccttc agctcgcgcc ccaggcggta gaaattttcg tacagggcgt
     2281 cccggagcgc gggatcggcg cgctctgcgc gccccgtgat ccccggagcg tggctgacca
     2341 cgccggcaaa cacgaccccc atcagacggc ccccctgccg tccgtggtca cgtaaagccc
     2401 ggcccggacg tctccgtgct cggcaaggcc gtcacgcatg gccttgatgt actcgtccca
     2461 cgcgtagccg cgcagcgcag cgtagtgcat gaggatctgg ccgttcacgc ccatcacgta
     2521 gagaagcccg atgtcgggct cccgaatggc gtggagctcc tcggcgtcga ggtcgtactc
     2581 ggccagcagc tcgtcgaaat cctcctcgta gcgtcggcgg gcgtgagggt cccgattcag
     2641 ctggtagaga agcttctgca cgtagtagag actcacggta gcctcacccc ttgcccaggc
     2701 tgggcacacg gtgcttgccg agggatatgc acacgttctt ggtttccatg tagaaatcaa
     2761 agctgtagtc accgccgtcc cggccgatgc cgcttgcctt catgccgccg aacggggtcg
     2821 gcagatgacg gttgttttcc gagttgaccc agatcatgcc cgcatccagg tcgcgcgcaa
     2881 gccggtgcga tctgcccacg tcccccgtcc agacgtaacc ggccaggccg aact
//
LOCUS       DBNHQR010000101         2912 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_9316, whole genome shotgun sequence.
ACCESSION   DBNHQR010000101 DBNHQR010000000
VERSION     DBNHQR010000101.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_9316"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="AC16VR_02195"
     CDS             <1..813
                     /locus_tag="AC16VR_02195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6009993.1"
                     /translation="LTTTTEVENWPGGHAELQGPELMAQMAEHVERFEARVINDYITT
                     ADLEAKPIRLQGDSQYTCDALIIATGASAMYLGLPSEEAFLGRGVSACATCDGFFYRG
                     QAVAVVGGGNTAVEEALYLSNICSKVYLVHRRDELRAEKILQDRLFARENIEPVWNHT
                     LAEVVGDDSGVTGVRLDGTHGAEPMDLDVTGVFIAIGHRPNTEIFAGQLEMEGGYITT
                     RSGLDGNATATSTPGVFAAGDVADHVYRQAITSAGFGCMAALDAEKYLDESV"
     gene            complement(829..1695)
                     /locus_tag="AC16VR_02200"
     CDS             complement(829..1695)
                     /locus_tag="AC16VR_02200"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF016032.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042398 - modified amino acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HIR6009994.1"
                     /translation="MPSTCTPRAFGELHNASLDVSIDTGYPPVINSRREAELAFRAAR
                     DVVGDAGLMQLDHPSMGAEDFSYYLERIPGCVLLMQVASSDLGKDLGQSVARPAESCR
                     TLGMRLVGAGTHPFSTRLAAITPGSRYESMERTSGLLGHRQITFSTHVHLSVIDGDEA
                     LWLARQLAPYLPLMLALSASSPFWQGHDTRFAAFRPRILASRFGLDARVAVNENGDVM
                     TLREVARQTLDLVADTAARFGDTDHLSRLARAISAGLPSERQRRRFAATGSLRAVVDA
                     VIDELLRDPVVG"
     gene            complement(1753..>2912)
                     /locus_tag="AC16VR_02205"
     CDS             complement(1753..>2912)
                     /locus_tag="AC16VR_02205"
                     /inference="COORDINATES: protein motif:HMM:NF015644.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HIR6009995.1"
                     /translation="QVPERVQRLAPFLVFDEDPYIVAVDGRLKWIVDAYTSSSYYPYS
                     QAFDSEEFFGLSRDEGQARVRTVQRLDGANYVRNSVKAVIDAYDGSVDFYVFDEEDLI
                     IQVWQRALPGLFKPRSEMPASLVAHVRYPQDFLLAQGLIFTRYHMSDPDVFYNQEDVW
                     VRATEKHYHDITPVEPYYVMWELPGSDEAEFVLILPFTPKNRQVLIGWVAALSDGENY
                     GRFIAYKFPKERRVLGPQQVETKIDQDSFLSGQLTLWDQQGSEVIRGNVLAIPIDDSL
                     LYVEPIYLRAETAAYPELRLVAVMQGDYLSYAETLDEALLGLFTGRQLVGETPSRSVK
                     GLAIEARMAFESYLEALGDKRFEAAGEALRRLSQLLERIDAAASVGEGNGP"
BASE COUNT          533 a          958 c          940 g          481 t
ORIGIN      
        1 ctgaccacga cgaccgaggt ggaaaactgg cccggcgggc acgctgagct ccagggcccc
       61 gagctgatgg cccagatggc tgaacacgtg gaacgcttcg aggcgagggt catcaacgac
      121 tacatcacca ccgcagacct cgaggccaag cccattcgcc tccagggaga ctcccagtac
      181 acgtgcgatg ccctgatcat cgcgaccggc gcgtcagcca tgtatctcgg gctgccttcg
      241 gaggaggcct ttctgggtcg cggcgtgagc gcctgcgcga catgcgacgg tttcttctat
      301 cgcggccagg ccgtcgccgt ggtcggcgga ggcaacacgg ccgtggaaga ggcgctgtac
      361 ctgtcgaaca tctgcagcaa ggtctacctc gttcaccgcc gcgacgagct gcgcgcggag
      421 aagatccttc aggaccgcct gtttgcccgg gagaacatcg agccggtatg gaaccacacc
      481 ctggccgagg tggtcggtga cgattccggc gtcaccgggg tgcgcctgga cggcacccat
      541 ggtgccgagc cgatggatct ggacgtgacg ggcgtgttca tcgccatcgg ccaccggccg
      601 aacaccgaga tcttcgccgg ccagctcgag atggagggcg gctacatcac cacccgcagc
      661 ggtctggacg gcaacgccac cgcgaccagc accccggggg tattcgcagc cggggacgtg
      721 gccgatcacg tctaccgcca ggcgattacc tccgccgggt tcggctgcat ggccgcgctg
      781 gacgccgaaa aatatctgga cgagtcggtc tgagcggcag gcccggtatc atcccaccac
      841 ggggtcccgc agaagctcgt cgatgacggc atccaccacc gcccgcaacg agccggtcgc
      901 cgcaaaccta cgccgctggc gttccgatgg gagccctgcg gagatcgctc gtgccagcct
      961 ggaaaggtgg tccgtgtctc cgaaccgcgc agccgtgtcg gctaccagat cgagcgtctg
     1021 acgtgcgacc tcgcgcagcg tcatcacatc gccgttctcg ttgacggcca cccgggcgtc
     1081 gaggccgaaa cgagaggcca ggatccgcgg ccggaaagcg gcaaaacggg tgtcgtgacc
     1141 ctgccagaat ggcgagctcg ccgagagcgc cagcatcagc ggcaggtaag gggccagctg
     1201 cctggcgagc catagggcct cgtccccatc gatgacgctc aggtgaacgt gggtagaaaa
     1261 ggtgatctga cgatgcccga gcagccccga cgtccgctcc atggattcgt atcgggagcc
     1321 tggcgtgatg gccgcaagcc tggtggagaa cggatgcgtc cccgcgccga ccagccgcat
     1381 tcccaacgtc cggcagcttt cggcaggtcg ggcgacgctt tggccgaggt ccttgccgag
     1441 atcgctcgac gcgacctgca tgaggagaac gcagccggga atccgttcca ggtagtagga
     1501 gaaatcctca gcacccatgc tcgggtgatc gagctgcatc aggccggcgt cgccaaccac
     1561 gtcgcgagcg gcccgaaagg caagctcggc ctcccggcgg ctgttgatca ccggcgggta
     1621 gcccgtgtcg atggatacgt ccagggacgc gttgtgcagc tcgccgaacg cccggggcgt
     1681 gcacgtcgat ggcaaatcgg tccgattggg ccgtgatcgt accggcgttc accgccgaat
     1741 cgacccattg cgtcacggcc cgtttccttc cccaaccgac gctgccgcat cgatccgctc
     1801 gagcagctgg ctgagccggc gcaacgcctc gcccgccgct tcgaagcgct tgtctcccag
     1861 cgcctcgaga tagctttcga aagccattcg cgcctcgatg gccaaccctt tgacggaccg
     1921 agacggcgtc tccccgacca gttgccggcc cgtgaagagg cccagcagcg cctcatccag
     1981 ggtctcggcg tagctcagat agtctccctg catcacggca accagtcgca gctcaggata
     2041 cgcggcggtc tcggcgcgaa gatagatggg ctcgacgtac aggagcgagt cgtcgatggg
     2101 tattgccagg acgttcccgc gtatcacctc ggagccctgc tggtcccaga gcgtcagctg
     2161 ccccgacagg aacgagtcct gatcgatctt ggtttcgacc tgctgaggcc cgagcacccg
     2221 ccgttccttg ggaaacttgt aagcgatgaa gcgcccgtag ttctcgccat cggagagcgc
     2281 ggcaacccag cctatgagca cctgccggtt tttgggcgtg aacggcagga tgagcacgaa
     2341 ttccgcttcg tccgagcctg gcagctccca catcacgtaa tagggctcca ccggcgtgat
     2401 gtcgtgatag tgcttctcgg tggctcgcac ccaaacgtct tcctggttgt agaagacgtc
     2461 gggatcggac atgtggtagc gggtgaagat gagcccctga gcgagcagga agtcctgggg
     2521 ataacgcacg tgggcgacca ggctggccgg catttcggag cggggcttga acaggcccgg
     2581 cagcgcgcgc tgccacacct gaatgatgag gtcttcttcg tcgaacacgt agaagtccac
     2641 gctgccgtcg taggcgtcga tgacggcttt gacagagtta cgcacgtagt tggcgccgtc
     2701 gagccgctgc accgtccgca cccgcgcctg accctcgtcc cgactcagcc cgaagaactc
     2761 ttcgctgtcg aaggcctggc tgtaagggta gtagctcgaa ctcgtgtagg cgtccacgat
     2821 ccacttcaga cgcccgtcca cggcaacgat gtaggggtcc tcgtcgaaga ccagaaacgg
     2881 ggcgagccgt tgcacccgct ccggcacctg ac
//
LOCUS       DBNHQR010000102         2502 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_452455, whole genome shotgun sequence.
ACCESSION   DBNHQR010000102 DBNHQR010000000
VERSION     DBNHQR010000102.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_452455"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /locus_tag="AC16VR_02210"
     CDS             <1..1152
                     /locus_tag="AC16VR_02210"
                     /inference="COORDINATES: protein motif:HMM:NF013345.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HIR6009996.1"
                     /translation="DVALKLLNERSWKPVPLAEPIPVSSLPTPALLIDESALDRNIKR
                     MADFVAARGKNARPHGKTHKCPIIAARQIEAGAVGICAAKVSEAAVFVNAGVEPVLIT
                     SPVTTAAKAELIVALARKGRVSVVVDGPLALQVLREAACPDAEVGVLVDIDVDMGRTG
                     CRDPDQVLALAESIETTPGLRYEGVQHYAGHLMHVEGHQNRRRRSTALWEQVAGVVDR
                     LRQNGLAPAVVTGGGTGTYDIDTDIEAVTDVQVGSYVFMDHEYRIIGGARGEVFDDFE
                     VALTVAATAISKPLDSAVTVDAGYKAFASDTVAPEPMHIAGARYRFAGDEHGVVIPAA
                     GSQEPLLGEVHQFITSHCDPTVNLYDHYWVHRDGLAHSIWPVAARGCSW"
     gene            1184..1807
                     /locus_tag="AC16VR_02215"
     CDS             1184..1807
                     /locus_tag="AC16VR_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017519262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR6009997.1"
                     /translation="MLEIVQSGGWLMVPILLCSIIAAAISVERLWTLQRGRIAPTDLL
                     AEVWDAIKGDRLDAQRLKDIRASSPLGQVLAAGIGNAKRGREIMKEAMEESAAQVSHD
                     MERYLTSLGVIASISPLLGLLGTVVGMIKVFTALMLEGAGNANVLAGGISQALITTAA
                     GLSVAIPALMFHRFFLRRVDELMVTMEQEAGKLVEIMQGGPDDDPRL"
     gene            1823..2248
                     /locus_tag="AC16VR_02220"
     CDS             1823..2248
                     /locus_tag="AC16VR_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018403010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR6009998.1"
                     /translation="MKFTRRRQPEATVELTPLIDVVFLLLIFFMVSTTFIRETQLKIN
                     LPEASGEIQEVERDFIEITIDRRGEYAVNDRLLVNAELRTLIRALEEMVAERGESRRV
                     IVTADAQASHQAVVRAMDAAGRVGLTQISLTTKQPEEEQ"
     gene            2277..>2502
                     /locus_tag="AC16VR_02225"
     CDS             2277..>2502
                     /locus_tag="AC16VR_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6009999.1"
                     /translation="MSDEAPAPADSPPSGGDWSTYRRLLGYVRPYWFVFLLAILGFLV
                     GSAAEAYFVKLFGDLIDNWDDIVVHASWAIP"
BASE COUNT          445 a          757 c          842 g          458 t
ORIGIN      
        1 gacgttgctt taaagctgct caacgagcga tcctggaagc cggtcccgct tgccgagccg
       61 atcccggtca gctccctgcc gacgcccgcg ttgctgatcg acgagagcgc gctggatcgc
      121 aacatcaagc gcatggcgga cttcgtcgcc gccaggggca aaaacgcccg cccacacggc
      181 aaaacccaca agtgtccgat catcgcggcg cggcagatcg aagccggtgc cgtggggatc
      241 tgtgcggcga aggtttccga agccgccgtc ttcgtcaatg ccggcgtgga acctgttctg
      301 atcacctctc ctgtcactac cgcagcgaag gccgagctca tcgttgcgct tgcccggaaa
      361 ggccgtgtga gcgtggtggt agacggcccg ttggcactgc aggtgcttag agaggcagcc
      421 tgtccggatg cagaggtggg cgtgctggtg gacatcgatg tggacatggg gcgcacgggc
      481 tgtcgggatc ctgatcaggt gctcgcgctc gcggagagca tcgagacgac cccgggcctg
      541 cgctacgagg gtgtgcagca ctatgcgggg catctgatgc acgtcgaagg ccaccagaac
      601 cgacgccgga ggtcgacggc gctctgggag caggtcgccg gggtcgtcga tcgccttcgc
      661 caaaacggcc ttgccccggc agtggtcacc ggcggcggta ccgggactta cgacatcgac
      721 acggacatcg aggctgtcac cgacgttcag gtgggcagct atgtgttcat ggatcacgaa
      781 tatcggatca ttggcggtgc ccggggcgag gtcttcgacg acttcgaggt ggcattgacc
      841 gtcgctgcca cggccatcag caaaccgttg gacagcgcgg tgacggtgga tgcgggctac
      901 aaggcgttcg cctcggatac cgtggcgccc gagcccatgc acattgcggg tgcgcggtac
      961 cggtttgccg gagacgagca cggcgtcgtc atcccggcgg cgggaagcca ggagcccctg
     1021 ctcggagagg ttcatcagtt cataacctca cattgtgatc cgactgtgaa cctctacgac
     1081 cactactggg tgcaccggga tggattggcg catagcatat ggcccgtcgc ggcgcgcgga
     1141 tgctcctggt aggggcgcga aaaatagcaa tggaggctac ggcgtgttag agatcgttca
     1201 atccggtggc tggctgatgg tgccgatcct gttgtgcagc atcattgcgg ctgcgatcag
     1261 cgtagagcgg ctctggacgc ttcagcgtgg gcgcatcgct ccaaccgatc tcttagctga
     1321 ggtctgggat gccatcaagg gcgaccggct ggacgctcag cgtctgaagg acattcgggc
     1381 ctcgagcccg ctcgggcagg tgctcgccgc aggtatcggc aatgccaagc gcggccggga
     1441 gatcatgaaa gaagccatgg aggagtcggc cgcacaggtg agccacgaca tggagcgcta
     1501 cctcacctcg ctcggggtca ttgcgtccat ctcgccgctg ctgggcctgc ttgggaccgt
     1561 ggtgggtatg atcaaggttt tcaccgccct catgctggaa ggagccggca acgccaacgt
     1621 gctcgccgga ggcatttccc aggcgctgat cacgaccgcc gccggtctct ccgtcgccat
     1681 tcctgcactc atgttccacc gtttctttct gcggcgggtg gacgagctca tggtgaccat
     1741 ggagcaggag gcgggcaagc tggtggagat catgcagggc gggccggatg atgatccgcg
     1801 gctctaacgg ggtttgccgt cgatgaaatt cacgcgacgc cgacaacccg aagccaccgt
     1861 cgagctgacg ccgctcatcg acgtggtgtt tctgctgctc attttcttca tggtgtcgac
     1921 gacgttcatt cgcgagacgc agctgaagat caaccttccg gaagcatctg gcgagattca
     1981 ggaggtggag agagatttca tagaaatcac catcgacagg cgtggcgagt acgcggtcaa
     2041 cgaccggctt ctggtcaacg cggagctccg cacgctgatc cgggccctcg aggaaatggt
     2101 cgccgagcgg ggcgaatcgc ggcgcgtgat cgtcacggcc gacgcgcaag cctctcatca
     2161 ggcggtcgtc cgagccatgg atgccgctgg ccgggtgggt ctgacccaga tcagcctcac
     2221 caccaagcag ccggaggagg agcagtagtc gtgcccagcc ggcgtggcgc gcgcagatga
     2281 gcgacgaagc gcccgccccg gcggatagcc ctccgtccgg cggagactgg agcacgtatc
     2341 ggcggctgct cggctacgta cgtccctact ggttcgtgtt tctgctggcc atcctcggct
     2401 ttctcgtcgg cagcgccgcc gaggcttact tcgtcaagct cttcggtgac ctgatcgaca
     2461 actgggacga catcgtcgtg cacgccagct gggccattcc gg
//
LOCUS       DBNHQR010000103         9251 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_832992, whole genome shotgun sequence.
ACCESSION   DBNHQR010000103 DBNHQR010000000
VERSION     DBNHQR010000103.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9251)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9251)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9251
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_832992"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="AC16VR_02230"
     CDS             complement(<1..1043)
                     /locus_tag="AC16VR_02230"
                     /inference="COORDINATES: protein motif:HMM:NF019843.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6010000.1"
                     /translation="MLAAALAAALAATLAATPAAAQAPAQEPPLDSRSFIERSVTLLK
                     EEEYALARTYLAPALIDYRLTPGERSRAYYLRGFTFFAEGFYVSAGKDYARALEFNPD
                     NPVALAAMADLYFRGLGVEADYVIAYQLYSKAAADGHANAQFHTGYLELEGIGIEKNL
                     DSARGWLHKAADQDVVPAMIHMARSYRTEATDSPDPDQARAWYERAFAAGSADALVAL
                     AFMHQDGDFGAPDPEKALELFTRAAQEGSGAAQVSLAHMHITGELLPQDYAKALELLQ
                     QAARQGVPASYLGLGHIYDTGLGVKPDRATAENWYRLAAEAELLPAQLRLVRLLLRED
                     DSTSDREAMAWLA"
     gene            1164..1521
                     /gene="ssrA"
                     /locus_tag="AC16VR_02235"
     tmRNA           1164..1521
                     /gene="ssrA"
                     /locus_tag="AC16VR_02235"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            1682..3358
                     /locus_tag="AC16VR_02240"
     CDS             1682..3358
                     /locus_tag="AC16VR_02240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HIR6010001.1"
                     /translation="MISDVTWMPGQKPFWWACCLIVCWLIQPAVADDDGRGRPYFKGS
                     PANPTALMRRMEADAHSAQALSAASSVGCVDGFADGFPCHNVHLQALVPLSTAGLSSA
                     SANDVWGWTDSISGREFALIGLNDGTGFVEITDPANPVVLGKLPTRNWNSTWRDIKTY
                     SDHAFIVSEADGHGMQVFDLTRLLDASPDITEAAHYDAFGSAHNIVINEDSGYAFAVG
                     TDTCSGGLHMVDISNPTNPRGAGCYSSAGYTHDAHCVVYQGPDPNYFGREICINSNED
                     TLVIVDVTDKAAPAVLSQVTYPGSGYTHQGWLTPDHSYFVFGDELDEQRFRHNTRTRV
                     FKLENLDQPAYAGSHDGITPAIDHNQYVLGNHVYQANYRAGLRILELTDPSAAAFSEV
                     AYFDVYPSSNSASYNGAWSVYPYFPSGNVVVSGIEQGLFVLRPDLAGGPPPSGDPPAA
                     PIGLAVTDQGDGTALIEFTDESDNETAFEVDREMQHKRNGWRGFTTISLDADTTSYAD
                     VSGSGTFRYRVRAVNAFGASDPTAWVQVTVTTSGGAGDGGGTKCHPKRGC"
     gene            3413..3703
                     /locus_tag="AC16VR_02245"
     CDS             3413..3703
                     /locus_tag="AC16VR_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR6010002.1"
                     /translation="MTIDGVATDGAICFVDSEAGTLFLSGTESPHERSSSPPQGSAGG
                     GQPGLVLLHHMDVVPATPAAWSTNPLQAVVVDGYLHGRGVQMLIELVTAFTR"
     gene            complement(3693..4667)
                     /locus_tag="AC16VR_02250"
     CDS             complement(3693..4667)
                     /locus_tag="AC16VR_02250"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR6010003.1"
                     /translation="MNELPKEATDQTDRGVRDLITAVSRVSAWHDSAIEYEKVTGGIT
                     NENFKVRADGATFFVKIPGSGTEAFIDRANCHVANQIAAEVGIGPAVAHFFPDTGVEI
                     FDWLDGYRTMNWGDVYDNDVFLGTADLIRKFQTFEDLQMPLVQTAFEQTFEMIRMARE
                     MGTYLPPEMDRMEYLAHQIEAAVERGGIEYRPCHNDYYSMNILIHERTGELKLVDYEY
                     ASMNDECYDMGVYSGANYFTEEMDVMFIKRRYGGWDEERFARLKLYKLLADIKWSMWA
                     LVQDRIATVRFDYFNWFGTKMARLRGNSLDPRIDYWLNLVGRTGRLNA"
     gene            complement(4813..5178)
                     /locus_tag="AC16VR_02255"
     CDS             complement(4813..5178)
                     /locus_tag="AC16VR_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010004.1"
                     /translation="MLASACQSTTAEQLPAVLIGDRLEAIAEITEVIADALRTEVSLS
                     PSAFSESSLLTLEHAVGSDARGSAATGLLLERPEQFRLLWSDDGCSLTRLRTGETLPL
                     DAAGCRPEAEATAGRAQSP"
     gene            complement(5207..6793)
                     /locus_tag="AC16VR_02260"
     CDS             complement(5207..6793)
                     /locus_tag="AC16VR_02260"
                     /inference="COORDINATES: protein motif:HMM:NF015531.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR6010005.1"
                     /translation="MTSNFPTTVTRRLASLLALAAIFAVLGGCGGGGGGSSPAVAVAP
                     EPEPEPEQWQPGVFEPASNFKDMCASPRIGAQFPDTQGTTTDENNWLRSWSNDLYLWY
                     EEIEDRDPARYTTPDYFDLLKTFASTPTGAPKDRFHFTYPTQEWEALSQSGQQPGYGA
                     AFVLLAAAPPRELAVAYIEPASPAEAAGLVRGARVLEIDGVSVVNGSDVDTLNAGLYP
                     SAAGEEHVFVVQEPGGGSRTVTLTSAIVTEDPVQFVKVIDSLTGPVGYLHFTRHIATA
                     ERELIDAIEFLGNAGVTDLILDVRYNGGGFLDIANELAFMIAGGGAASGRVFAEIQFN
                     DKHQTVNPVTGAVLEPEYFHTTTQGFSAEAGDPLPTLNLDRLYVLTTADTCSASESIV
                     NGLRGIDLDVVQIGSPTCGKPYGFYATDNCGTTYFSIQFKAVNAKGFGDYPDGFAPED
                     GAPAGAVTLPGCGVDDDFDNPLGDPAEALLATALEHRAGLGCPVGTATAARAEPRLAR
                     KGSGGEPLTPPRPYGMVLAR"
     gene            complement(6818..7966)
                     /locus_tag="AC16VR_02265"
     CDS             complement(6818..7966)
                     /locus_tag="AC16VR_02265"
                     /inference="COORDINATES: protein motif:HMM:NF019640.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6010006.1"
                     /translation="MHVQPIISEIARQADAADRSRTVDPDVIAAIKRSDVMGLSASAG
                     LGGLDAPVTEIARELGAVAPACSSTAWCLWNHLSTFHLFAGLLGPEHADFLKDIVERH
                     EWVCFPAGASTAVRAEEHDGEFSLRGTAAFGSGARYAEWSGVSFVPAEGGPPEFSMVD
                     LRSEGVRIDPTWEAMSLRASATDHVHYEGVRVPAERRVPFHFRYREVFRNPDHAMIDA
                     RYREDWVALSDLWLGAMAVGVAQACLDECCDGIRERVAILGTKMAERPTIHVNLGQAA
                     AAINAARDTVFAALSETDERLDSSVIPDEADYLRQVSASMQALQLCDESMRLMLRVLG
                     GNGLREGSNFERRYRDFQAMPLHINAHRDRITEQVGRHLLGLETENPF"
     gene            8078..>9251
                     /locus_tag="AC16VR_02270"
     CDS             8078..>9251
                     /locus_tag="AC16VR_02270"
                     /inference="COORDINATES: protein motif:HMM:NF014795.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR6010007.1"
                     /translation="MQSESVASRYLSLLAERGISHLFVNAGTDFAPLVEAYARSGDAQ
                     GPALPAPVLCTHENLAVGMAHGAYLVSGRPQAVMFHVSVGTANAVCAVTNAARDNVPL
                     LVTAGRTPILEDGALGARDTHIHWAQEMFDQAGMLRELVKWDYELRDPRQVHQVVDRA
                     LSMATSHPRGPVYLSLPREVLAEQMPDADAGARRTAVPVAVEPESSAIEELAGRIAAA
                     RCPVIVTSASGCEPDSVAVLADLADRFAIGIAEMMPRYLNAPMDHELHLGTQMQSIFD
                     AADLIVFLECDVPWIAARAAPRPETFIVQAGTDPLYSRYPMRTHRSDLTLAATPTALL
                     TGLADALASRAGQVDPDRRARLVELALRGKAAIAKLKAAEAEREGTITRAGLSAVLG"
BASE COUNT         1568 a         2988 c         3059 g         1636 t
ORIGIN      
        1 ctggcgagcc acgccatcgc ttcccggtcg gaggtgctgt cgtcctcccg gagcaggagc
       61 ctgaccaggc ggagctgcgc cgggagcagc tcggcctcag ccgccagtcg gtaccagttc
      121 tcggccgtcg cgcgatcggg cttcacgccc aggcccgtgt cgtaaatgtg cccgagcccc
      181 aggtagctgg ccggcacccc ctgccttgcc gcctgctgaa gcagctccag ggccttggcg
      241 tagtcctgtg gcagcagctc gccggtgatg tgcatgtgcg ccaggctgac ctgggcggcc
      301 ccggagcctt cctgggcagc ccgggtgaag agctccagcg ccttttcagg gtccggcgcg
      361 ccgaagtccc cgtcctggtg catgaaggcc agagccacca gcgcgtctgc gcttcccgcg
      421 gcgaaagccc tctcgtacca ggctcgggcc tggtccggat ccgggctgtc ggtggcctct
      481 gtccggtagc tgcgtgccat gtgaatcatg gcgggcacca cgtcctgatc tgcggctttg
      541 tgcagccatc ccctggcact gtccaggttc ttctcgatgc cgataccttc gagttccagg
      601 taaccggtgt ggaactgggc gttcgcgtgg ccgtcggcgg ccgctttgga gtagagctgg
      661 taggcgatga cgtaatccgc ctccacgccc aggccccgga agtagaggtc ggccatggcg
      721 gccagggcca cggggttgtc cgggttgaac tccagggccc gcgcgtagtc tttgcctgcg
      781 gagacgtaga agccctcggc gaagaaggtg aagccgcgca ggtagtaggc gcgggagcgc
      841 tcgcccggtg tcaggcgata gtcgatgagt gccggcgcga gataggttct ggccagcgcg
      901 tactcttctt ctttcagcag cgttaccgac cgctctatga agctgcggga atccagcggc
      961 ggctcctggg ctggcgcctg cgctgcggcc ggtgtggcgg cgagtgtggc ggcgagtgcg
     1021 gcggcgagtg cggcggccag cacaggtttc aaaacaccaa aacggggcat gttgataggg
     1081 gaaccaattc tcgtgaaatc agtcagttat tatgcagtaa cgttatattt gacgcgtgat
     1141 acaattcgcg tcacctcgta aatgggggcg atttggcttc gacgggggta acggagcttg
     1201 cggtgcatgc cgagagggta gtgactctcg ttaaacaatc gctgtaactt aatatagttg
     1261 ccaacgacga aaactacgca ctagcggcct aaaaaacccg cttacgtcga cgagcttggc
     1321 ctgcaaggcg aagtcgacgg caaagtttgc aggatcgcgt ggacgtctcg cccggggctg
     1381 aagcgttaaa accaatcggg ctcgctgaca gcaccctgac cgtcgggtcg ctcgaggcta
     1441 aatcaataga cggaagctaa gcatgtagag ccaacagccg agtgccaacg gacgcgggtt
     1501 caactcccgc cgcctccacc aacatgcgat ccgggcaagt ccaggatcgt ccgcaaagcc
     1561 cgcccagtgc gggctttttt gtgcctctcg cgaacggatt gtggctgatc ggaacatgtt
     1621 gtaaatttag tttcttatat atctgagtga tcgctgtaag gtcggggtgg agatgagaag
     1681 catgatcagc gatgtcacct ggatgccggg gcagaagccg ttctggtggg cctgttgcct
     1741 catcgtttgc tggctgatcc agcccgccgt tgccgacgac gacggtcgcg gacgcccgta
     1801 cttcaagggt tctccggcca acccgacggc gctgatgagg cgcatggagg ccgatgcgca
     1861 cagcgcccag gcgctgtcgg cggcgagcag cgttgggtgc gtggacgggt ttgccgacgg
     1921 ctttccctgc cacaacgtcc acctacaggc gcttgttccg ctgagtacgg ccgggctctc
     1981 cagcgccagc gcaaacgacg tctggggctg gaccgactcg atctcgggac gcgagttcgc
     2041 cctgattggt ctaaacgacg gcaccggatt cgtggagatt accgaccccg ccaatccggt
     2101 cgttctggga aagcttccca ccaggaactg gaattcgacc tggcgcgata ttaagaccta
     2161 ctcggatcac gcgttcatcg taagcgaggc ggacgggcat ggcatgcagg tgttcgatct
     2221 gacgaggctg ctcgacgcct cgcccgacat caccgaggcg gcccattacg acgccttcgg
     2281 cagcgcccac aacatcgtca tcaacgagga cagcggctac gcgtttgccg tcggcaccga
     2341 cacctgcagt ggcggtctgc acatggtgga catctcgaat cccacgaacc ctcggggcgc
     2401 tggatgctat tcgagtgcgg ggtataccca cgacgcgcac tgcgtcgtct accagggtcc
     2461 ggatccgaat tacttcggtc gggagatctg catcaacagc aacgaagaca ccctcgtcat
     2521 cgtcgatgtg accgacaagg cggcacccgc tgtcctcagc caggtgactt atccagggtc
     2581 cggttatacc catcagggct ggctcacgcc tgaccatagc tacttcgtct tcggcgacga
     2641 gctcgacgag cagcgctttc gacacaacac ccggacgcgc gttttcaagc tggagaacct
     2701 cgaccagccg gcctatgcgg gcagtcatga cggcatcacg cccgccatcg accacaacca
     2761 gtacgtcctg gggaaccacg tctaccaggc caactaccgg gcgggtctgc gcatcctgga
     2821 gctcaccgat ccttccgctg cggcgttctc cgaggtcgcc tacttcgacg tctaccccag
     2881 cagcaactcg gcttcgtaca acggcgcttg gagcgtctat ccctactttc ccagcggcaa
     2941 cgtggtggtc agcggcatcg agcagggact cttcgtgctc aggccggacc tcgcgggcgg
     3001 accaccacca tcgggagatc cgccggcagc gccgatcggg ctcgcagtca ccgaccaggg
     3061 cgatggcacc gcgctcatcg agttcaccga cgaaagcgac aacgagaccg ccttcgaggt
     3121 cgatcgcgag atgcagcaca aacgcaacgg ctggcgcggt ttcacgacga tcagcctgga
     3181 cgctgatacg acttcgtacg ccgatgtcag cggctcgggc acgttcagat accgcgtccg
     3241 tgcggtgaat gcctttggcg cgtcagatcc gaccgcttgg gtgcaggtca ccgtgaccac
     3301 ctccgggggc gcaggcgatg gcgggggcac caagtgccac ccgaagagag ggtgctagca
     3361 ctgcgggcgg atcgggcggt gccttgggca tcagcactca tctgcaaagt cggtgacgat
     3421 cgacggcgta gcgacagacg gcgccatctg tttcgtcgac agcgaagcgg gcacgctctt
     3481 cctctcagga accgagtctc cgcatgaacg ctcatcgagt cctcctcaag ggagcgctgg
     3541 cggtggccaa cccggcctcg tgctgctgca tcatatggac gtcgttccgg cgacgcccgc
     3601 agcctggagt accaacccgc tgcaggcggt ggttgtcgat ggctatctgc acggtcgcgg
     3661 cgtgcagatg ctgatcgagc tcgtgaccgc gtttacgcgt tgagccggcc cgtgcgacct
     3721 acgaggttca gccagtagtc gatgcgcggg tcgagggagt tgccgcgcag cctcgccatc
     3781 ttggttccga accagttgaa gtagtcaaac ctgacggtgg cgattcggtc ctggaccaat
     3841 gcccacatgg accacttgat gtcggccagc agcttgtaga gcttcagacg cgcgaagcgc
     3901 tcctcgtccc aaccgccata gcgacgtttg atgaacatca cgtccatctc ttcggtgaag
     3961 tagttggccc cggaatagac gcccatgtca tagcactcgt cgttcatgga cgcgtactcg
     4021 taatccacca gcttcagctc gccggtgcgt tcatggatga ggatgttcat cgagtaatag
     4081 tcgttgtggc aggggcggta ctcgatgccg ccgcgttcga cggccgcctc gatctgatgg
     4141 gcgaggtatt ccatccgatc catctcgggc ggcaggtagg tgcccatctc gcgcgccatg
     4201 cggatcatct cgaacgtctg ttcgaatgcg gtctggacca ggggcatctg cagatcttcg
     4261 aacgtctgga attttctgat gagatcggcc gtgccaagga agacatcatt gtcgtacacg
     4321 tcgccccagt tcatcgtccg gtaaccgtcc agccagtcga agatctcgac gcccgtgtcc
     4381 gggaagaaat gggcgaccgc cggaccgatg ccgacctcgg cggcgatctg attggcgacg
     4441 tggcagttcg cccggtcgat gaaggcttcg gtcccgctac cggggatctt gacgaaaaaa
     4501 gtggcgccat cggcgcggac tttgaagttc tcgttggtga ttccaccggt caccttctcg
     4561 tactcgatgg cgctatcgtg ccaagcgctt acgcgtgaca ccgccgtgat caggtcgcga
     4621 acgccccgat ccgtctgatc cgtcgcttcc ttcggcagct cattcatccc gcttgtccct
     4681 gataaggtgt cgatgctggc agccaggagg tttgcaagtc ccggcgggca aacccagtct
     4741 agtacagaaa gcacgccggc gcaggacgct gacgccacgg cttgccgccg ctggccgaat
     4801 cctgattgaa agctaagggg attgtgcacg gcccgctgtc gcctccgcct cgggacggca
     4861 gccggcggca tccagcggca gggtctctcc ggtgcgcagg cgcgtaaggg agcagccgtc
     4921 gtccgaccac agcagccgaa actgctcggg ccgctcaagg agcaatcccg tggcagcact
     4981 tccccgggca tcggagccca cggcgtgctc cagcgtcaaa aggctcgatt ctgagaaagc
     5041 cgacggagac agcgaaactt ccgtgcgcag cgcgtcggcg atcacctcgg tgatctccgc
     5101 gatggcctcg aggcggtcgc cgatcagaac cgccgggagc tgctccgcag tggtgctctg
     5161 acaagcggac gccaacacga gcgggatcag gcagattttt ctcatctcaa cgtgccagca
     5221 ccatgccgta tggccttggc ggcgtgagcg gctcgccacc cgagcctttg cgggccagac
     5281 gcggctccgc cctggcggct gtggccgtgc ccaccgggca gcccaggcct gcgcggtgct
     5341 ccagcgccgt ggccagcagg gcctcggccg ggtcgccgag cgggttgtcg aagtcgtcgt
     5401 cgacgccgca cccgggcagc gtcacggcgc cggcaggggc gccatcttcc ggggcgaagc
     5461 cgtccgggta gtcgccgaac cccttcgcgt tgacggcttt gaactgaatg gaaaaatacg
     5521 tcgtcccgca gttgtccgtg gcgtagaagc cgtagggctt gccgcaggtg ggcgatccga
     5581 tctgcaccac gtcgaggtcg atgccgcgca gcccgttgac gatggactcg gaggccgagc
     5641 aggtgtcggc ggtggtgagc acgtagagcc ggtccaggtt caacgtgggc agggggtcgc
     5701 cggcctcggc ggagaagccc tgggtggtgg tgtgaaagta ctccggctcg aggaccgcgc
     5761 cggtgacggg gttgaccgtc tggtgcttgt cgttgaactg gatctcggca aacactcggc
     5821 cgctggccgc gccgccgccg gcgatcatga aggccagctc gttggcgatg tccaggaaac
     5881 cgccgccgtt gtagcggacg tccaggatca gatcggtgac gccggcgttc cccagaaatt
     5941 cgattgcatc gatcagctct cgctcggcgg tggcgatgtg gcgggtgaag tgcagatagc
     6001 ccacgggacc ggtcagagag tcgatcacct tgacgaactg gaccgggtcc tcggtgacga
     6061 tggcggaagt cagggtgacc gtgcgcgagc cgccgccggg ttcctgcacg acgaagacgt
     6121 gctcctcgcc ggcggccgac gggtaaagcc ccgcgttcag cgtgtccacg tccgatccgt
     6181 tgaccacgga gacgccgtcg atctccagta cccgggctcc ccggacgagg cctgccgctt
     6241 ccgcggggct ggcgggctcg atgtacgcca ccgccagctc ccggggcggc gctgcggcca
     6301 ggagaacgaa ggcagccccg tagcccggtt gctggccgga ctggctcagg gcttcccact
     6361 cctgggtggg gtacgtgaag tggaagcggt ccttgggcgc gcccgtcggt gtggaggcaa
     6421 acgtcttcag caggtcgaag tagtcggggg tcgtgtaccg cgcggggtcc cggtcctcga
     6481 tctcctcgta ccacagatac agatcgttgc tccaggagcg cagccagttg ttctcgtccg
     6541 tcgtcgtccc ctgggtgtcg ggaaactgcg cgccgattcg cggcgatgcg cacatgtcct
     6601 tgaagttgga cgccggctcg aagacgcccg gctgccactg ttccggctcg ggctcgggct
     6661 ctggggctac cgcgacggcg ggcgaggagc caccgccccc gcccccgcac ccgcccagaa
     6721 cggcgaagat cgcggcgagg gccaggagcg aggccagtcg ccgggtaacc gtcgtcggaa
     6781 aattgctcgt catgacgcag aggttggacc cttcgattca gaaggggttc tcggtttcga
     6841 gccccagcag gtgcctgccg acctgctcgg tgatgcggtc cctgtgtgcg ttgatgtgca
     6901 ggggcatggc ctggaagtcg cggtagcgcc gctcgaagtt cgaaccctcc cggaggccgt
     6961 tgccgcccag gacgcgcagc atgagccgca tggactcgtc gcacagctgc agggcctgca
     7021 tggaagcgct cacctggcgc agatagtccg cttcgtccgg aatgacgctg gaatcgagcc
     7081 tctcgtcggt ttccgacagg gcggcgaaca ccgtgtcgcg ggccgcgttg atggcggcag
     7141 cggcctggcc cagattcacg tggatggtcg ggcgttcggc catcttggtc ccgagtatgg
     7201 caacccgctc gcggatgccg tcgcagcact cgtcgaggca ggcctgggcc accccgaccg
     7261 ccatggcgcc gagccagagg tcagacagcg ctacccagtc ctcccggtag cgcgcgtcga
     7321 tcatggcgtg gtcgggattg cggaaaactt cccggtaacg gaagtggaag ggcacccggc
     7381 gctcggccgg cacgcgcacc ccctcgtagt gcacgtggtc ggtggcggac gcgcgcaggc
     7441 tcatcgcctc ccaggtgggg tcgatgcgca cgccctcgct gcgcaggtcg accatgctga
     7501 actcgggcgg cccgccctcg gcgggtacga agctcacgcc gctccactcc gcatagcggg
     7561 cgccggagcc gaacgcggcg gtgccgcgca ggctgaactc gccgtcgtgc tcctctgccc
     7621 ggacggccgt gctggcgccg gcgggaaagc agacccactc gtgccgctcg acgatgtcct
     7681 tgagaaaatc cgcatgctcc gggccgagca acccggcgaa gagatgaaac gtgctcagat
     7741 gattccacag gcaccacgcg gtagagctgc acgcgggcgc caccgcgccg agttcccggg
     7801 cgatctccgt cacgggcgcg tccagcccgc cgagaccggc gctcgcgctc agccccatga
     7861 cgtccgaacg cttgatggcc gcgatgacgt ccgggtccac ggtgcgggac cggtcggccg
     7921 catcagcctg cctggcgatt tccgaaatta tgggttgaac gtgcacgctt cctcccgagc
     7981 cgggcatgat cgcccggcgc gtagctgcgg taaagtgata gcctaggcgg ccgccgcgaa
     8041 ttgggctcgg cgagtgatcg tcacacggag aaagggcatg cagtcggagt cggtcgcatc
     8101 acggtatctt tcgctcctcg ctgagcgtgg gatcagccac ctctttgtca acgcgggaac
     8161 cgacttcgcg cccctggtcg aagcgtacgc ccgctccggt gatgcccagg gccctgcgct
     8221 gccggcaccc gtgctctgca cccacgagaa cctggccgtg ggcatggccc acggggccta
     8281 cctcgtcagc gggcggcccc aggcggtgat gttccacgtc agcgtcggca ccgccaacgc
     8341 ggtgtgcgcc gtcaccaacg ccgcgcgcga caacgttccg ctgctggtga ccgccggccg
     8401 gacgcccata ctcgaagacg gtgcgctggg tgcgcgagat acccacattc actgggccca
     8461 ggagatgttc gatcaggccg gcatgctgcg cgagctggtc aagtgggact acgagctgcg
     8521 cgatccccgg caggttcacc aggtggtgga cagggcattg agcatggcga cctcgcaccc
     8581 gcgcggcccg gtctacctct cgttgccgcg ggaggtgctc gcggagcaga tgccggacgc
     8641 ggatgctggg gctcgaagga cggcggtacc ggttgccgtg gagccggaat cctcggctat
     8701 agaggagctg gccggccgga tcgcggccgc acgttgtccg gtgatcgtca cctcggcgtc
     8761 cggctgcgag cctgacagcg tggccgtgct tgccgatctg gccgaccgct ttgccatcgg
     8821 catcgccgag atgatgcccc gttacttaaa cgcccccatg gaccacgagc ttcacctcgg
     8881 cacccagatg caatccatct tcgacgcggc ggatctcatc gtcttcttgg agtgcgacgt
     8941 gccctggatc gcggcgcgcg cggccccgcg gccggaaact ttcatcgtcc aggccgggac
     9001 cgatcctctc tactcgcgtt accccatgcg cactcatcgc tccgacctca ccctggcggc
     9061 gacgcctacg gctctgctta ctgggctcgc ggatgctttg gcctcgcgcg ccgggcaggt
     9121 cgatcccgac cgccgcgccc ggctggtgga gctcgccctg cgtggcaaag cggccatcgc
     9181 gaagctaaag gcagcggaag cggagcgcga aggcaccatc acccgggcag gtctttccgc
     9241 cgtgctcggc g
//
LOCUS       DBNHQR010000104         6286 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1339271, whole genome shotgun sequence.
ACCESSION   DBNHQR010000104 DBNHQR010000000
VERSION     DBNHQR010000104.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6286)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6286)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6286
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1339271"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="AC16VR_02275"
     CDS             <1..752
                     /locus_tag="AC16VR_02275"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 -
                     branched-chain-amino-acid:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HIR6010008.1"
                     /translation="LFNSAHILGLKIPFSVDEVNAAQVDVFRANALNEGYLRPMVYLG
                     SEAMGLRAKDLSVNLCVAAWAWPDYMDPDSREKGINVRTSSFTRHHVNITMCKAKANG
                     NYINSILALHEALDSGCDEALLLDNEGYVAEGSGENVFIVKDGEVHTPELTSCLDGIT
                     RNTIFHLAAEMGIAIRERRITRDEVYIADEAFFTGTAAEVLPIRELDGRMIGSGKRGP
                     VTERFQSAYFDQVRGKRSAFPEWHTPVSGAQ"
     gene            742..1746
                     /gene="waaF"
                     /locus_tag="AC16VR_02280"
     CDS             742..1746
                     /gene="waaF"
                     /locus_tag="AC16VR_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017336199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HIR6010009.1"
                     /translation="MPNSGTRILIVAPAWVGDMVMAHSLVQVLIARHGDPGIDMLAPR
                     ATAGLGARMPGVRNVHVLDVEHGELALGARRSSGLTLKDQYDQAIVLPNSFKSALAPA
                     WARIPVRTGWSGEARVGVLNDRRRLNPERYPRMVDRFAALGLDPDAPLPDDIPGPVLE
                     PDAAAAERLLASRRLSVDGGVTALCPGAEFGPAKRWPSVHFAQVASYAAAQGHQVWLV
                     GSPKDAGVCSEVEAQSPAGVVNLAGRTTLLEAVDLLSRADRVVSNDSGLMHVASALGR
                     PVVGVFGSTSTTFTPPLGERATTVSLGIECSPCFQRECPLGHLRCLNDLPPQRVIELL
                     "
     gene            1746..2801
                     /gene="waaC"
                     /locus_tag="AC16VR_02285"
     CDS             1746..2801
                     /gene="waaC"
                     /locus_tag="AC16VR_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008016956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HIR6010010.1"
                     /translation="MHVLLIKMSSMGDVVHALPAVTDAASHGVTFDWVVEEAFADIPA
                     LHPAVGQVMPIAWRRWRSSLRSSRGELARFWEQLRRRRYDLVLDSQGLVKSGVVAALA
                     RGRTKAGFSHASARERAAALAYRRGVRVARNQHAIDRQRELFAAVFGYCVPAGLDYGI
                     ARAAHQASKTNHCVLLHGTTWESKHYPEHLWREVARRARSAGLDVALPWGSEAERARA
                     EAIAMTNADVLPALNLAELTTRIASARLVVGVDSGLAHLSAALDVPTVVIYGSTDSAL
                     TGCRGRRVSNLQAQFPCAPCLRRACGYHGPEEILHQAPVRPACYSRVTPDAVWDAAAR
                     AMADGGRETAPNPGSDL"
     gene            2825..4213
                     /locus_tag="AC16VR_02290"
     CDS             2825..4213
                     /locus_tag="AC16VR_02290"
                     /inference="COORDINATES: protein motif:HMM:NF018047.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HIR6010011.1"
                     /translation="MAELQAQGRDLPTPFILEGSLNPAPDDGPIECLEVLRVLPGRRI
                     VMRARLGARQCALKLFVGDRAALYARRERAGLELLAASGVDTPELIADLHLPGGGEGI
                     LVDWLAGSRPVDEEDVQGLADVAAVLARLHANGAIQHDAHLNNYLRDADGMLHAIDGD
                     GVRRPARLRRRQELANLALLLAQLPPPDATRLPVVAEAYWRARWGSDPGPDELAALQR
                     RVHAQRAGRMRRYLRKTLRDCSEFHGERKNGRFVVCARNAWTAQMAEFVDRPEDHLVA
                     GTVLKAGNSATVMRLRIGETAYVVKRYNVKGFFHGLRRALRPTPRYRVAWLSGHRLLR
                     LGIATPRPVALLEARQGLATVAYLVMEDLGAARDLAQAVAEEGYTAGVGRAVAGLFRG
                     LRDAGLVHGDTKASNFLVRDASVYLIDLDAMKPGSQTRSRDVARFLANWDDAPQVCES
                     FRGALQEAGVPL"
     gene            4238..5983
                     /locus_tag="AC16VR_02295"
     CDS             4238..5983
                     /locus_tag="AC16VR_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007939655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase family protein"
                     /protein_id="HIR6010012.1"
                     /translation="MPLIILGLSGALNHDPSAALLVDGEIVAAVEEERFLRDKHAKNR
                     MPLESARYCLRAGGISPRDVDVVAFPYAPIALSSAARWHYARRHWYAPDRALDALFNG
                     NRRYRRNRRRVLELGDQLGIDWSRTRFEPVEHHLAHASSAYHLSGYTEKTAIMGVDGK
                     GEYATTFFGYGENGRIHRIKEFYDPDSLSGVYGAMTEYLGFEMLDGEYKVMGMAPYGD
                     PDLYDLGRLIRRARDGFEVDVNYVNTVGLRRHKESGVGFYFSDKLINWLGPRRQGEVA
                     DEPYIHYAASMQKLFEEWCLELMERYLGDVLRETGRLAFAGGGALNVKLNQRIVALDH
                     VRELFVQPAAGDSGTSLGAATYVAASEGQRLSPMTHVFLGPRYDNDDCLQALRARRES
                     LHWRRLDDVPARAAELLAQGHPLAWFQGRMEFGPRALGARSILGCPSTPGIADRINEQ
                     IKFRERWRPFCPSVLDRVAAEIIQTEHPAPFMTITFDVAQEWKSRIPEVVHEDGTARV
                     QIVDPDSNPRYYALLETLETLTGNAVVLNTSLNRRGEPMVCSPEDALNMFCGSDLQFL
                     VLEDFLVSKTDEEAQ"
     gene            complement(5977..>6286)
                     /locus_tag="AC16VR_02300"
     CDS             complement(5977..>6286)
                     /locus_tag="AC16VR_02300"
                     /inference="COORDINATES: protein motif:HMM:NF015251.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LpxL/LpxP family acyltransferase"
                     /protein_id="HIR6010013.1"
                     /translation="GPKHSVFAPFFGAPAASITATSRLARISGSPVIFASHFRDWNTM
                     TWHVTFSEPLDGFPTSDEVADAARVNQLIENAIRAHPDQYLWIHRRFKTRPPGEGKLY
                     "
BASE COUNT         1022 a         2050 c         2144 g         1070 t
ORIGIN      
        1 ggcttttcaa ctcggcgcac atcctcggcc tcaaaatccc cttcagcgtc gacgaggtca
       61 acgcagccca ggtcgacgtt ttccgtgcca acgctctgaa cgaaggctac ctgcggccca
      121 tggtgtacct gggttcagag gccatgggcc tgcgggccaa ggatctttcg gtaaatctct
      181 gtgtcgccgc ctgggcgtgg ccggattaca tggaccccga cagccgcgag aaggggatca
      241 acgtgcggac gtcgtcattc acccgccacc acgtgaacat caccatgtgc aaggcgaaag
      301 ccaacggcaa ctacatcaac tccattctcg ctcttcatga ggcgctggac agcggatgcg
      361 acgaggcgct gctgctcgac aacgagggat acgtcgccga gggcagcggc gagaacgtct
      421 tcatcgtcaa ggatggcgag gtgcacacgc cggagcttac ctcctgtctc gacggcatca
      481 cgcgaaatac gatcttccat ctggccgcgg agatgggcat cgccatcagg gagcggcgca
      541 tcaccagaga cgaggtgtac atcgccgacg aggcgttctt cacggggacg gccgccgagg
      601 tgctgccgat tcgcgagctc gatggtcgaa tgatcggctc gggcaaacgc gggcccgtca
      661 cggagcggtt ccagtcggcc tacttcgatc aggtgcgggg caaacgcagc gccttcccgg
      721 agtggcacac gcccgtaagc ggtgcccaat agcggcaccc gcatcctgat cgtcgccccc
      781 gcttgggttg gcgacatggt catggcgcac agcctcgtgc aggtcctcat cgcgcggcac
      841 ggcgaccccg ggatcgatat gctggcccct cgcgccacgg ccgggctcgg agcccgcatg
      901 ccgggcgtga ggaacgttca cgtgctcgac gtggagcacg gtgagctcgc cctgggcgcc
      961 aggcgcagct cggggctgac cctcaaggat cagtacgacc aggccatcgt gctgccgaac
     1021 agcttcaagt cggcgctcgc acccgcgtgg gccaggattc ccgttcgcac cggctggagc
     1081 ggcgaagcac gggtcggggt gctcaacgac cggcgccgcc tgaacccgga gcgctacccg
     1141 agaatggtcg accggttcgc ggctctggga ctcgatcccg acgcgccgct gccggacgat
     1201 attcccggtc ccgtcctcga gcccgatgcc gctgccgcgg agcgcctgct cgcctcccgc
     1261 cggctctcgg tggacggggg cgtgaccgcc ctgtgcccgg gggccgagtt cggcccggcc
     1321 aagcgctggc cgtccgttca ctttgcccag gtcgccagct acgccgccgc tcaaggacat
     1381 caggtatggc tcgtggggtc gccgaaagat gccggggtat gctcggaggt ggaggcgcag
     1441 tcgccggccg gcgtggtcaa cctcgcgggg cgcaccacgc tgctggaagc cgttgacctg
     1501 ctctccaggg ccgaccgggt cgtcagcaac gattcgggtc tcatgcacgt cgcctcggcg
     1561 ctcggccggc ctgtggttgg cgtcttcgga tcaacctcca cgaccttcac gccgccgctc
     1621 ggcgagcgag cgacgacggt gagcctgggt atcgagtgca gcccgtgctt tcaacgcgaa
     1681 tgcccgctgg ggcatctgcg ctgcctcaac gacctgccgc ctcaacgagt catcgagctg
     1741 ctctagtgca cgtcctgctg atcaagatgt cgtcgatggg ggacgtggtc catgctctgc
     1801 ccgcggtgac ggacgccgcg agccatgggg tgactttcga ctgggtagtt gaggaggcgt
     1861 ttgcggacat acccgcgctg cacccggcag tcggccaggt gatgcccatt gcctggcggc
     1921 gatggcgctc ctcgctgcgc tcgtcccggg gggagctggc ccggttctgg gagcagctcc
     1981 gtcgccggcg ctacgacctc gtcctcgatt ctcaggggct cgtcaaaagc ggcgtggtgg
     2041 cggcgctggc aagaggaaga accaaggccg ggttctctca cgcctcggct cgggaaaggg
     2101 ccgccgccct ggcttatcgg cgcggggttc gggtggcgcg caaccagcat gccatcgacc
     2161 gccaacgcga gctcttcgcc gcggtgttcg gctattgcgt ccctgccggg ctcgactacg
     2221 gaatcgcgcg ggcggcgcat caggccagca agaccaacca ttgcgtgctg ctgcacggaa
     2281 ccacctggga gagcaagcac tacccggagc atctttggcg ggaagtcgcc cggcgcgctc
     2341 gctcggccgg gctggacgta gctctgccct ggggctccga ggcggagcgt gcgcgggcgg
     2401 aagcgatcgc gatgacgaac gccgacgtcc tgccggccct gaaccttgcc gagctgacca
     2461 cgcgaatcgc ctcggctcgg ctcgtcgtgg gcgtggactc gggactcgct cacctcagcg
     2521 cggccctcga cgtgcctacg gtggtgatct acggcagcac ggacagcgca ctcacgggat
     2581 gccgcggccg cagggtgagc aatctgcagg cgcagtttcc gtgcgcgccc tgcctgcgca
     2641 gagcttgcgg ctatcacggc ccggaggaaa ttctgcatca ggcgccggtg cggcccgcgt
     2701 gctacagccg cgttacgccc gatgcggtgt gggatgccgc agcccgagcc atggcggatg
     2761 ggggtcggga gaccgcccca aacccggggt ccgacctgtg acggccacgg gccggccgct
     2821 ttctttggcg gagctgcagg cgcagggacg cgatctgccg acgccgttca ttctggaagg
     2881 atccctcaat ccggcccctg acgatgggcc gatcgagtgt ctcgaggtcc ttcgcgtgct
     2941 cccgggccgg cgaatcgtca tgcgagcgcg gctgggggct cgtcaatgcg cgctcaagct
     3001 ctttgttggg gaccgggctg ccctttacgc caggcgggag cgagccggcc tggaactgct
     3061 ggcggccagc ggggtggaca cgccggagct gattgccgac ctccatctcc ccggcggcgg
     3121 ggagggcatc ctggtggatt ggctggcggg cagccggccg gtggacgagg aagatgtcca
     3181 aggcctggcg gatgttgcgg ccgtgcttgc acgcctgcac gccaacggtg ccatccagca
     3241 cgacgctcat ctgaacaact acctccgcga tgcagacggc atgctgcatg ccatcgacgg
     3301 cgacggcgtt cgccggcctg cacgtcttcg gcgcagacag gaactcgcca acctcgccct
     3361 gctgctggcc cagctgccgc cgccggatgc gactcgcctc ccggtggtgg cggaagctta
     3421 ctggcgtgcg cgctggggat cggaccccgg cccggacgag ctggctgcgc tgcagcgacg
     3481 ggtgcacgct cagcgagccg gcaggatgcg ccgttacctc cggaagacgc tgcgggattg
     3541 cagcgagttt cacggcgagc ggaagaatgg ccgcttcgtg gtctgtgccc gaaatgcctg
     3601 gaccgcgcaa atggccgagt tcgtcgaccg tccggaagac cacctggtgg ccggcaccgt
     3661 gctcaaggca ggcaacagcg ccacggtgat gcgcctgcga atcggcgaaa cggcgtacgt
     3721 ggtcaagcgc tacaacgtca aaggtttctt tcacgggctg cggcgggcgc tgcgccccac
     3781 gccgcgctat cgcgtggcgt ggttgagcgg tcatcgcctg cttcgcctgg gtattgccac
     3841 gccccggccg gtcgctctgc tggaagcgcg ccaggggctt gctaccgtgg cctatcttgt
     3901 catggaggat ctcggtgcgg cgcgggatct ggcgcaggcc gtcgccgagg aaggatacac
     3961 cgcgggtgtc ggacgtgcgg ttgccgggtt gttccgcggg ctgcgcgacg cgggcctggt
     4021 gcacggggat acgaaagcca gcaacttcct ggtgcgcgac gcaagcgtct acctcatcga
     4081 cctggatgcc atgaagccgg gcagccagac gcggtcccgc gacgtcgccc gtttcctggc
     4141 gaactgggac gatgcgccac aggtgtgcga gtcttttcgc ggcgccttgc aggaggccgg
     4201 cgtgccgcta tagtacgcgc actttccgat cccaggtttg ccgttgatca tcctcggcct
     4261 ttccggagcg ctcaaccacg acccttcggc cgcgttgctt gtcgacgggg agatcgtcgc
     4321 ggcggttgaa gaggaacggt ttctgcgcga caagcacgca aagaaccgca tgccgttgga
     4381 atcggcccgc tactgtctcc gcgccggtgg gatttcgcca agggacgtgg acgtggtcgc
     4441 gtttccctac gcgccgatcg cgctgtccag cgctgcgcgc tggcactacg ccagacgcca
     4501 ctggtacgcg cccgatcggg cgctcgatgc gcttttcaac ggcaaccgtc ggtatcgccg
     4561 aaaccggcgg cgcgttttag agctcggcga ccagctcggc atcgactgga gccggacccg
     4621 attcgagccc gtggagcatc atcttgccca cgcctccagc gcctaccacc tgagcggcta
     4681 tacggagaag acggccatca tgggggtgga tggcaagggt gagtacgcca ccacgttctt
     4741 cggctacggc gagaacggcc gtattcaccg gatcaaggaa ttctacgacc cggactccct
     4801 gagcggcgtc tacggcgcga tgaccgaata cctcggcttc gagatgctcg acggtgagta
     4861 caaggtgatg ggcatggcgc cctacggaga tcccgaccta tacgatctcg ggcgcctgat
     4921 ccgccgagca cgcgacggct tcgaggtgga cgtgaactac gtgaacacgg tgggcctgcg
     4981 acgtcacaag gagtcgggcg ttggcttcta cttcagtgac aagctcatca actggctcgg
     5041 accacgccgg caaggggagg tggctgacga gccctacatc cactacgccg catccatgca
     5101 gaagctcttc gaggagtggt gcctggaact catggagaga tacctcgggg acgtgctgcg
     5161 ggagaccggg cggctggctt ttgcaggcgg cggagccctc aacgtgaagc tgaaccagcg
     5221 aatcgttgcc ctggatcacg ttcgtgagct gttcgtccag ccggcggccg gagactcggg
     5281 tacttcgctg ggggccgcca cctacgtggc agccagcgaa ggtcaacgcc tatcccccat
     5341 gacccacgtc tttctcggtc cgcgttacga caacgacgat tgccttcagg cccttcgagc
     5401 ccgtcgcgag tcccttcact ggcggcgcct ggacgacgtg cccgctcggg cggcggaact
     5461 tcttgcccag gggcatccgc tggcgtggtt ccagggccgc atggagttcg gcccccgggc
     5521 cctgggcgcc cgcagcattc tgggttgtcc cagcacgccc gggattgccg atcgcatcaa
     5581 cgagcagatc aagttccgcg agaggtggcg gcctttctgc cccagcgtgc tcgatcgggt
     5641 cgcagcggaa atcatccaga ccgagcatcc cgcgcctttc atgaccatta ccttcgatgt
     5701 ggcgcaggaa tggaaatcgc ggatcccgga agtcgttcac gaggacggga ccgcccgtgt
     5761 ccagatcgtg gatccggaca gcaatccccg gtactacgcg ttgctcgaga ccctggaaac
     5821 gctcacgggc aacgcggtgg tgctcaacac gtctctcaac cggcggggag aacccatggt
     5881 ctgctcgccg gaagacgcgc tgaacatgtt ctgcgggtcc gatcttcagt ttctcgtgct
     5941 cgaggatttt ctcgtcagca agacggacga ggaagctcag tagagctttc cctcgcccgg
     6001 gggacgcgtc ttgaaccggc gatgaatcca gaggtactga tccgggtgcg cgcggatcgc
     6061 gttctcgatc aattggttga cccgtgccgc gtcggccacc tcgtcgctgg tcgggaaccc
     6121 atcgagcggc tcgctgaacg tgacgtgcca ggtcatcgtg ttccagtcgc gaaaatggct
     6181 ggcaaagatc acgggcgagc cggagatccg cgcgaggcga gacgtggcgg tgatggaggc
     6241 ggcgggcgcg ccgaagaaag gcgcaaagac ggagtgcttg ggcccg
//
LOCUS       DBNHQR010000105         2491 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1592700, whole genome shotgun sequence.
ACCESSION   DBNHQR010000105 DBNHQR010000000
VERSION     DBNHQR010000105.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1592700"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="AC16VR_02305"
     CDS             <1..239
                     /locus_tag="AC16VR_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HIR6010014.1"
                     /translation="CRASGTGAELDGASIPVMAGAKLEHALFGGDDYELCFTAAAEPG
                     ELGVACTRIGTVVAGSEITLDARPVSAAGYQHFR"
     gene            239..727
                     /locus_tag="AC16VR_02310"
     CDS             239..727
                     /locus_tag="AC16VR_02310"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF016489.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006644 - phospholipid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A family protein"
                     /protein_id="HIR6010015.1"
                     /translation="MSTISWRDARDPAVLISCGFGSGFLPGAPGTWGSLAATALWWPL
                     LGGALWWVQAAGALLVLIAGTLLIGRVHRRHDVGDAPAIVIDEFAGMWLVLIGTGQDP
                     LLVAAAFGLFRLFDIWKPWPVRAVEARVRGGLGVMLDDVIAGAMALFVLQVSIWAIGG
                     GF"
     gene            779..1423
                     /locus_tag="AC16VR_02315"
     CDS             779..1423
                     /locus_tag="AC16VR_02315"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease family
                     protein"
                     /protein_id="HIR6010016.1"
                     /translation="MKYLVRVLCVFSLVMPINAIASALPVEVVGLFKNHAVVRMQGGE
                     FVMKAGETRGGVTLVSADARQARMAYQGREHLVGLSERVSSQYRAVDKAQVMISSDSL
                     GQYWIRGAINNRFTNFLVDTGASVVAISAVHARQLGIEFDESHQGTVQTAQGTADTHF
                     VTLDEIVVGGITAHNVRAAIIEGEYPLEPLLGMSFLRQVSLQENAGVLTLTKQH"
     gene            complement(1432..2313)
                     /locus_tag="AC16VR_02320"
     CDS             complement(1432..2313)
                     /locus_tag="AC16VR_02320"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014576115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR6010017.1"
                     /translation="MTPELDELRALINDALDHHLPSSSAIAQPLVDAMRYTTLGGGKR
                     IRPMLVCATCTGLGGALEDALAPACAVEFIHAYSLIHDDLPSMDDDELRHGLPSSHIK
                     FGEATAVLAGDALQALAFETLSRAPSGSDAQRLRAVQLLARAAGPQGMVGGQSYDLES
                     ERKKLSLSQLQTLHAAKTGALITAAVEIGALFGKPDLSPEKHVLISEFAARIGLAFQV
                     IDDILDVTATTEKLGKPAGSDTGAGKSTFPSLIGVESARGMAEELLAEALPMLDRVGI
                     KHSLLKDLAELIVKRAF"
     gene            complement(2310..2489)
                     /locus_tag="AC16VR_02325"
     CDS             complement(2310..2489)
                     /locus_tag="AC16VR_02325"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011531978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HIR6010018.1"
                     /translation="MESMESGEMSLEESLRAFERGIKLTRHCQSALKAAELKVRMLTE
                     NDELVALDADDPPPE"
BASE COUNT          361 a          803 c          860 g          467 t
ORIGIN      
        1 tgtgccgtgc cagcggcacc ggggccgagc tcgacggcgc atcgatcccg gtcatggccg
       61 gcgcgaagct cgagcacgcg ctctttggcg gcgacgatta cgagctgtgc ttcaccgccg
      121 ccgcggagcc cggggagctc ggtgtcgcct gcaccaggat cggcaccgtg gtggcggggt
      181 cggaaatcac gctggacgct cggccggttt cggccgccgg ctaccagcac tttcgctgat
      241 gagcactatc tcctggcgcg acgcgcgcga ccccgcagta ttgatctcct gcggcttcgg
      301 cagcggcttt ctgccgggag cgccgggtac ttggggcagc ctggcggcga cagcgctctg
      361 gtggccgctc cttggcggtg cgctctggtg ggtccaggcc gccggggcct tgctggtgct
      421 aatcgcgggc acgctgctca tcggccgggt gcaccgacgc cacgacgtcg gtgatgcgcc
      481 cgccatcgtc atcgacgagt ttgccggcat gtggttggtg ctgatcggta ccggccagga
      541 tcctctcttg gtcgccgccg ccttcggcct gtttcgcctg ttcgacatct ggaagccctg
      601 gccggttcgt gccgtggaag cccgcgtgcg cggaggcttg ggcgtcatgc tcgatgacgt
      661 catcgccggc gccatggcgc tttttgtgct gcaggtctca atctgggcca ttggcggcgg
      721 gttttagcgt cgaggctgac tacactcgaa ggccgtcaag tcgcgttcac ggacgaccat
      781 gaaatacctc gtgcgggttc tctgcgtgtt ctctctcgtc atgccgatca acgcgatcgc
      841 ttcggctttg cccgtggagg tggtggggct gttcaagaac cacgccgtgg ttcgcatgca
      901 gggcggcgag ttcgtgatga aagcgggcga gacccgcggc ggggtgaccc tcgtctccgc
      961 ggatgccagg caggcgcgca tggcctatca gggccgggag cacctcgtcg ggctgtccga
     1021 acgcgtttcc agtcaatacc gcgcggtcga caaggcccag gtgatgatca gctccgattc
     1081 attgggccag tactggattc gcggcgccat caacaatcgc ttcaccaact tcctcgtcga
     1141 caccggcgcg tcggtggtcg ccatcagcgc ggtgcatgcc cggcagctgg gcatcgagtt
     1201 cgacgagtcg caccagggca cggtgcagac cgcgcagggc actgccgaca cgcatttcgt
     1261 cacgttggat gagattgtgg tcggtggcat cacggcccac aacgtgcgcg ccgccatcat
     1321 cgaaggcgag tacccgctcg agccgctgct gggcatgtcg ttcctgcgcc aggtgagcct
     1381 gcaggaaaac gcgggggtgc tcaccctcac caagcagcac tgagcgaggg ttcagaaggc
     1441 ccgcttgacg atcagctccg cgagatcctt gagcagcgag tgcttgatac cgacccgatc
     1501 cagcatgggc agcgcttcgg ccagcagctc ttcggccatc ccgcgcgcgg actcgacgcc
     1561 gatcagcgaa ggaaacgtgc tcttgcctgc tcccgtgtcg gagccggctg gtttgccgag
     1621 cttttccgtg gtggccgtga cgtccaggat gtcgtcgatg acctggaaag cgagaccgat
     1681 cctggcggcg aactcggaga tgagcacgtg tttctcgggg gaaagatcgg gcttgccgaa
     1741 aagcgcgccg atctccaccg ccgccgtgat cagcgctccg gtcttggccg cgtgcagggt
     1801 ctgcagctgg ctcaggctca gcttcttccg ctcggattcc agatcgtagc tctgcccgcc
     1861 caccatcccc tggggtccgg cggctctggc gagcagctgc acggcgcgca gccgctgggc
     1921 gtcggacccc gagggtgccc tcgacagagt ctcgaacgcc agcgcctgca gggcgtcgcc
     1981 ggcgagcacc gccgtggcct cgccgaactt gatgtggctg ctgggcagcc cgtggcgcag
     2041 ctcgtcgtcg tccattgacg gcaggtcgtc gtggatcagg gagtaggcgt ggatgaactc
     2101 caccgcgcac gccggcgcca gcgcgtcttc cagcgccccg ccgagcccgg tgcaggttgc
     2161 gcacaccagc atcgggcgga tgcgtttgcc tccgcccagg gtggtgtaac gcatggcgtc
     2221 caccaggggt tgggcgatgg cggaagaact gggaaggtgg tgatcgagcg cgtcgttgat
     2281 gagcgcgcgt agctcgtcga gttcgggagt cattccggtg gcgggtcgtc ggcgtccagc
     2341 gcaacgagct cgtcgttctc ggtgagcatg cgtaccttga gctccgccgc tttcagcgcg
     2401 gactggcagt gccgggtcag cttgatgccg cgttcgaagg cgcgcagcga ttcctccagg
     2461 gacatctcgc cggactccat gctttccacg a
//
LOCUS       DBNHQR010000106         7772 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_326470, whole genome shotgun sequence.
ACCESSION   DBNHQR010000106 DBNHQR010000000
VERSION     DBNHQR010000106.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7772)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7772)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7772
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_326470"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="AC16VR_02330"
     CDS             complement(<1..718)
                     /locus_tag="AC16VR_02330"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR6010019.1"
                     /translation="MQPVERHVDVATPDGSMNTFIAYPQGGGPFPVVVFYMDAPGKRE
                     ELHDMARRLASAGYYVMLPNLYYRRVREFVMTAEARPEMMAHMNSLSNHMACEDTAAL
                     FAFASADRAARDGPAGCVGYCMSGPFAFAAAAAFPDRIAAAASIHGVRLCTDAIDSPH
                     LNAEHVGGELYFACAEHDDWAPPEMIEALEGHLSRVGASYRIEWYPGTSHGFVFPARP
                     VYHPAAAERHWERVLDLFSRN"
     gene            complement(720..1526)
                     /locus_tag="AC16VR_02335"
     CDS             complement(720..1526)
                     /locus_tag="AC16VR_02335"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6010020.1"
                     /translation="MADLKGKVAVVTGASRGVGRGVAEGLAEAGATVYVTGRSESHDN
                     PPQERTIQATAASVDALGGKGIAVRVDHNEDAEVKALFERVMSEQGRLDVLVNNVYKI
                     PDPPVFDGGFWEHPVSVWDDQCGVGLRGYYVASVFAAPMMVARRSGLIVNISSRGGDQ
                     YVFSTSYGVGKAGVDRMARDMAHELRGYHVAAVSLRPSSVKTEFILDAVASGAVKLDM
                     AKAQSPRFTGRCVAALAMDPDLMEKSGGVYMVADLAAEYRFTDPDRVEAS"
     gene            1658..2068
                     /gene="fur"
                     /locus_tag="AC16VR_02340"
     CDS             1658..2068
                     /gene="fur"
                     /locus_tag="AC16VR_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HIR6010021.1"
                     /translation="MPGSDIKRAGLKVTLPRLKVLEVLESAEPHHLSAEDVYKSLIDS
                     DDSVGLATVYRVLTQFETAGIVSRHNFDDGHSVYELAGDEHHDHMVDMDTGQVIEFVN
                     ERIEALQREIAQEHGFELIDHELVLYVRKQGKSE"
     gene            2177..2401
                     /locus_tag="AC16VR_02345"
     CDS             2177..2401
                     /locus_tag="AC16VR_02345"
                     /inference="COORDINATES: protein motif:HMM:NF015956.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HIR6010022.1"
                     /translation="MSLRDLSPGDMARVSGYREDDDYAAQLMRLGLIPGTEIRVQRRA
                     PLGDPMEIRFRGFSLALRPSEADALMLELI"
     gene            2398..4491
                     /gene="feoB"
                     /locus_tag="AC16VR_02350"
     CDS             2398..4491
                     /gene="feoB"
                     /locus_tag="AC16VR_02350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HIR6010023.1"
                     /translation="MTTVALVGNPNCGKTTLFNALTGTRQRVGNWPGVTVERKTGSYF
                     HGDGSVDVVDLPGTYSVHPVGDAVDERIAREFISAAEPSGPDVVVNVVDASSLARGLY
                     LTTELLQMGKPVVVALNMIDVADRNGMHIDTFQLARELGCPVVALVASRGDGVGALKD
                     AVESREGAGPASALPPMSEPDERFHYVDGLITRCVKATPTRRSVTDRIDAVVLHRYLA
                     YPIFLGVMYLMFMFAINVGSAFIDLFDGVGNVLFVEGPRRAMLAVGLPEWLVVFAADG
                     VGGGIQLVGTFIPVIGCLFLALSFLEDSGYMGRVAFIVDRLMRRLGLPGKSFVPLIVG
                     FGCNVPAVMATRTLDNEPDRILTTIMAPYMSCGARLTVYALFAAAFFPTGGQNVVFAL
                     YLIGIVVAVLSGLAVRRYLISDSRSTFLLELPTYHLPTMKGLLLQTWQRLKGFVLRAG
                     KAIVAVVIVLNVVGSVGTDGSFGNQNTEKSVLSALGRTITPVFHPMGISQENWPATVG
                     IFTGIFAKEVVVGTLDALYAPGEVASADDLAGQLRAAAASVPANLAELGEQLTDPLGI
                     GLGALDDVAEAASDQEVEINTIQAMRELFHGKLGAFSYLLFILLYMPCVATIGAIFKE
                     IGGFWAAFSTSWSVTVAYSAAVICYQLGNLAEQPGPAMLWIAGVAAATVAAFAGLVAM
                     GRRRRQRLIPLVNLE"
     gene            4608..5861
                     /locus_tag="AC16VR_02355"
     CDS             4608..5861
                     /locus_tag="AC16VR_02355"
                     /inference="COORDINATES: protein motif:HMM:NF019042.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HIR6010024.1"
                     /translation="MRVVVVMAAMALVSSATAEEVTLEQLWAVVQAQQAQIEALTDAL
                     ETTQAQLASTEEQVEVAEEQIGLTADYLAEIEASSTRGSRTQLGGYGELHYNNVDARD
                     PDESFKEMDFHRFVLFFSHEFTDRIRFHSELELEHSLVKDTADGSNGGEVELEQAYVE
                     MDLNENHWTRAGLFLLPVGILNETHEPNTFYGVERNDVESIIIPSTWWEGGVAAGGRY
                     GNGLSWEAGVHSGLAMPTDGGNAFRVRSGRQKVSNASAENLAYTLRLKYTGIPGLELA
                     GSYHHQSNPSQMDEDGLDGGDLFSFHGIWSQGPFTLKGLWARWNFDGDLVESSGADRQ
                     TGWYLEPSVRLDLAGTDWGFYGRYEDLEGGRTQDRFDQWEVGFNFWPTENVVLKFDYR
                     DRDHDLETELGRDFKAIDLGVGYQF"
     gene            5933..6457
                     /locus_tag="AC16VR_02360"
     CDS             5933..6457
                     /locus_tag="AC16VR_02360"
                     /inference="COORDINATES: protein motif:HMM:NF016121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HIR6010025.1"
                     /translation="MIIGCVLTLFGPALLAGAAVYQKPDEFLAEAFVGDAPSAQALWI
                     KAELKHELSQVLAHPPGLRVRYWQAGSRTAWILDEIGKDLPITAGIVINDGAIEDVRV
                     LEFRESRGWEIRYPFFTRQFQNARLQGDRLTQNIDGITGATLSVRAMKRMATAALLLH
                     EHSSASSTTLAKAP"
     gene            6498..7151
                     /locus_tag="AC16VR_02365"
     CDS             6498..7151
                     /locus_tag="AC16VR_02365"
                     /inference="COORDINATES: protein motif:HMM:NF015863.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HIR6010026.1"
                     /translation="MLVLVYLLATGVPLQYAAELDLGQRHVASEWVLDWYGLQAPDEA
                     TRSGPAVMLGGTLFLDGNEQAGLERLVGAIPAGGILAVASSDEVWLFHPGVPDPERTA
                     FRPNIRRIGSLDGQIYLDTTEGLLAADGLLMNWTPTNAPAGAIEWGRVTPLSAGEAAA
                     YRQRFRGRMLTAERWLQDLHSGRFFGTPGVIIVDVASLLMLILAGTGIVLWWRSRRG"
     gene            complement(7176..>7772)
                     /locus_tag="AC16VR_02370"
     CDS             complement(7176..>7772)
                     /locus_tag="AC16VR_02370"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6010027.1"
                     /translation="ELSQVKSLAAVRIFADGNNVVVREDNQVWDVETGQGQLDFTVRE
                     LADNVATLAQRNLIVLRESDGLDSDEWYNLGLDLEEVDPDRAPDAYSRAIELNPKNAD
                     AHVNLGRLMQLKGNLKFAKRHYELALVAVSNHQLAYYNLGTVYDELDDMDKAAEYYRR
                     APGVPDAHFNLSRIYQMQGDEVSALRHMRQYRELLDVE"
BASE COUNT         1341 a         2551 c         2510 g         1370 t
ORIGIN      
        1 ggttccggct gaagagatcg agaacccttt cccaatgccg ctcggctgcc gccgggtggt
       61 agacgggccg tgcggggaag acgaagccat gagaagtgcc cggataccac tcgatccgat
      121 agctggcgcc cacccgggaa aggtgcccct ccagggcttc gatcatctcc gggggagccc
      181 agtcgtcgtg ctcggcgcag gcgaagtaaa gctcgccccc aacgtgctcc gcgttcagat
      241 ggggagaatc gatcgcgtct gtgcaaagcc tcacgccgtg aatggaggcg gccgcggcaa
      301 tgcgatcggg aaaggcggca gccgcggcaa aggcgaaggg tccgctcatg cagtagccca
      361 cgcagccggc cgggccatcc cgcgccgctc ggtctgccga tgcgaaggca aacagagcgg
      421 cggtgtcctc gcaggccatg tggtttgaaa gcgagttcat gtgggccatc atctccggcc
      481 gggcttcggc ggtcatgaca aactcccgga cgcgccggta gtagaggttc ggcagcatga
      541 cgtagtagcc ggccgacgcc agccgtctgg ccatgtcgtg cagctcctcg cgcttgcccg
      601 gcgcgtccat gtagaacacc accacgggga acggaccgcc cccctggggg tacgcgatga
      661 acgtgttcat ggacccgtcg ggcgtggcaa cgtccacgtg acgttccacc ggctgcacct
      721 cagctcgcct ccacgcggtc cgggtcggtg aaccggtact cggccgcgag gtcggccacc
      781 atgtagacgc cgcccgactt ctccatgaga tccgggtcca tggccagcgc ggccacgcac
      841 cgcccggtga accgcggcga ctgcgccttg gccatgtcga gcttgaccgc gccgctggcc
      901 accgcgtcca gaatgaattc ggtcttcacc gaggagggtc gcagggacac ggcagcgaca
      961 tggtagccgc gcagctcgtg ggccatgtcc cgagccatgc gatcgacccc cgccttgccc
     1021 acgccgtagg aggtgctgaa cacgtactgg tcgccgcccc gtgaggagat gttcacgatg
     1081 agaccgctgc gccgggcgac catcatcggc gcggcgaaca cggaggccac gtagtagcct
     1141 cgaagcccca ccccgcactg atcgtcccag acggataccg ggtgctccca gaagccgcca
     1201 tcgaaaaccg gcggatcagg aatcttgtag acgttgttga ccaggacgtc gagcctgccc
     1261 tgctcgctca tgacgcgctc gaagagagct ttgacctcgg cgtcttcgtt gtgatctacc
     1321 cgcacggcaa ttcccttgcc gccaagggca tcgacgctcg ccgcggtcgc ctgaatggtg
     1381 cgctcctgag gcgggttgtc gtggctctcg ctgcgcccgg tgacgtagac ggtggcgccg
     1441 gcctcggcca ggccttcggc tacgccccgg cctaccccgc ggctcgcacc ggtaaccact
     1501 gccaccttgc ctttcagatc tgccacgtga tcgtcctccc gatgtcgtta ccccgtgccg
     1561 acggcgattg taacccagcc actccacgcc ccatggtcac gggtggacgg tcagccctgc
     1621 atgggctagt gtccccaact accgttgggg aaagccgttg cccggatccg acatcaaacg
     1681 tgccggcctg aaggtcacgc ttccgcgcct gaaggtgctg gaggttctgg agagcgccga
     1741 gccgcaccat ttgagcgccg aagacgtcta caagagtctc atagattcag acgattctgt
     1801 gggtctggcc accgtttacc gggtgctgac ccagttcgag accgccggca tcgtatcccg
     1861 ccacaacttc gatgacggcc actcggtgta cgagctggcc ggcgacgagc atcacgatca
     1921 catggtggac atggacaccg gtcaggtcat cgagttcgtc aacgagcgga tcgaggcgct
     1981 gcagcgggag atcgcccagg agcacggatt cgagctcatc gaccacgagc tcgtgcttta
     2041 cgtgcgcaag cagggaaagt cggagtaagc ggacgagccc tctgccgccc cacctgctgc
     2101 caattgccaa tgagaatgat ttttatttaa atggcgggcc gttccccacg gcccgaaccg
     2161 gaggccaccg ttgtccgtgt cactacgcga tctttcaccc ggagatatgg cccgcgtcag
     2221 cggctaccgg gaggatgacg actacgcagc ccagctgatg cggctcggcc tcattccggg
     2281 caccgagatc agagtgcagc gccgtgctcc cctgggggac ccgatggaga tccgcttccg
     2341 cggcttctcc ctggcattgc ggccatccga ggccgacgcg ctgatgctgg aactgatatg
     2401 accaccgtcg ccctggtggg caatcccaac tgcggcaaga cgacgctgtt caacgcgttg
     2461 acgggcacgc gtcagcgcgt aggcaactgg ccgggcgtta cggtggagcg caaaacgggc
     2521 agctacttcc acggcgacgg ttccgtggac gtggtcgacc tgcccggcac ctacagcgtg
     2581 catcccgtcg gagacgcggt ggacgagcgc atcgcccggg agttcatcag cgccgctgag
     2641 ccatcgggcc cggatgtcgt cgtcaacgtg gtggacgcat cgagtctcgc ccgcgggctc
     2701 tacctgacca ccgagctgct gcagatgggc aagccggtgg tcgtcgcgct gaacatgatc
     2761 gacgtcgccg accgcaacgg gatgcacatc gacaccttcc agctcgcccg ggagctcggc
     2821 tgcccggtcg tcgcgctggt ggcaagccgg ggggacggcg tgggggccct caaggacgcc
     2881 gtcgagagca gggaaggcgc cggccccgcc tcggccctgc cgcccatgag cgagcccgac
     2941 gagaggttcc actacgtcga cgggctgatt acccgctgcg tcaaagccac accgacccgc
     3001 cgctccgtga ccgaccggat cgacgccgtg gtgctccatc gctacctggc ctacccgatc
     3061 tttctgggcg tcatgtatct catgttcatg ttcgccatca acgtgggctc tgcgttcatc
     3121 gacttgttcg acggggtcgg caacgtgctg ttcgtcgagg gtccccgtcg cgcgatgctg
     3181 gccgtgggcc tgccggaatg gctcgtcgtg ttcgcggcag acggtgtcgg cggcggcata
     3241 cagctggtcg ggaccttcat tccggtgatc gggtgcctgt ttctcgcgct gtcgtttctc
     3301 gaggacagcg gctacatggg gcgcgtcgcg ttcatcgtcg accggctcat gcgacgcctg
     3361 ggccttccgg gcaagtcgtt cgtgccgctg atcgtgggct tcgggtgcaa cgtgccggcg
     3421 gtgatggcga ctcgcacgct ggacaacgag ccggaccgca tcctcaccac gatcatggct
     3481 ccctacatgt cctgcggggc ccggctgacg gtctacgcgc ttttcgccgc ggcgttcttc
     3541 cccaccggcg ggcagaacgt ggtattcgcc ctctacctga tcggcatcgt cgtcgcggtg
     3601 ctgtcgggat tggcggttcg acggtacctg atctccgaca gccgaagcac gttcctgctg
     3661 gagctgccaa cgtaccacct gccgacgatg aaggggctgc tgctgcagac ctggcaacgg
     3721 ctcaaaggct tcgtcctgcg cgccggcaag gccatcgtgg ccgtggtgat cgttctcaac
     3781 gtggtgggat cggtaggcac cgacggctcg ttcgggaatc agaacaccga gaagtctgtg
     3841 ctctccgctc tggggcgtac catcaccccg gtctttcatc ccatgggcat cagccaggaa
     3901 aactggccgg ctaccgttgg catcttcacc ggcatcttcg ccaaggaggt cgtggtcggg
     3961 actctggatg ccctgtacgc accgggcgag gtggcgtccg cggacgacct cgccggccag
     4021 ctccgggcag ccgccgcttc ggtccccgcc aatcttgccg agctcggcga gcagctcacc
     4081 gacccgctgg gcatcgggct aggggccctg gacgacgtgg ctgaggccgc ctccgaccag
     4141 gaggtcgaga tcaacaccat tcaggccatg cgcgaactct ttcacggcaa gctcggcgcg
     4201 ttcagctacc tgctgttcat cctgctgtac atgccctgcg tggccaccat cggcgccatc
     4261 ttcaaggaga tcggcggctt ctgggcggcg ttcagcacga gctggtctgt gaccgtcgct
     4321 tactcggccg cggtcatctg ctaccagctg ggcaacctcg ctgagcagcc gggcccggcc
     4381 atgctgtgga tcgcaggcgt cgcggcggcc accgtcgccg ccttcgcggg gctggtcgcc
     4441 atgggccgca gacgtcggca acgcctgatt ccgctggtca acctggaata ggcccacggc
     4501 gctgcggcaa gaccaatacc ttaatctaaa taataataat tcctattcat aaacacatcc
     4561 gctagactcc cgttcccctt gatccaacac gttcgggaga atcgctcatg cgtgtcgtcg
     4621 tggttatggc cgccatggcc ctggtttcgt ccgccaccgc cgaggaagtc accctggagc
     4681 agctgtgggc cgtggtgcag gctcaacagg cccagatcga agcgctgacc gacgcgctcg
     4741 aaaccaccca ggcccagctc gccagcaccg aggagcaggt ggaggtcgcc gaggagcaga
     4801 tcggcctcac cgccgactac ctcgcggaga tcgaggcgtc cagcacgcgc ggcagccgca
     4861 cccagctggg cggttacggc gagcttcact acaacaacgt cgacgcccgc gatccggacg
     4921 agagtttcaa ggagatggac ttccatcgct tcgtgctgtt cttcagtcac gagttcaccg
     4981 accggatccg gttccactcg gagctggagc tggagcattc actggtcaag gacaccgcag
     5041 acggcagcaa cggcggcgag gtggagctgg agcaggccta cgtggaaatg gacctcaacg
     5101 agaaccactg gaccagggct ggtctttttc tgctgcccgt cggcattctc aacgagaccc
     5161 acgagccgaa caccttctac ggcgtcgagc gcaacgacgt ggagagcatc atcatcccgt
     5221 ccacctggtg ggaggggggc gtggcggccg gggggcgcta cggcaacggc ctgtcctggg
     5281 aggccggcgt gcacagcggc ctggccatgc ccaccgacgg cggcaatgcg tttcgcgtgc
     5341 gcagcggccg ccagaaagtg tccaacgcca gcgccgaaaa tctcgcctac accctgcgcc
     5401 tgaagtacac cgggattccc gggctcgagc tggccggcag ctatcaccat cagagcaacc
     5461 ccagccagat ggacgaagac ggtctcgacg gcggcgacct gttcagcttt cacggcatct
     5521 ggagccaggg gcctttcact ctgaaaggcc tctgggcgcg ctggaacttc gacggcgatc
     5581 tcgtcgagtc ctcgggcgcc gatcggcaga cgggctggta cctggagccc agcgtccggc
     5641 tcgatctggc cggcaccgac tggggattct acggccgcta cgaagatctc gaaggcggtc
     5701 ggacccagga cagattcgat cagtgggaag tcggcttcaa tttctggccc acggagaacg
     5761 tggtgctgaa attcgactac cgagatcgcg accatgacct ggagacggag ctcgggcgcg
     5821 acttcaaggc catcgacctg ggcgtcggct accagttctg agcagccgtc ttacaagccc
     5881 ggaattgatt tttgcaccga tcacggcaaa atcgacccat ctgaaaataa gaatgattat
     5941 cggttgtgtt ctcacccttt tcgggccggc cctgctggcc ggcgcggccg tgtatcagaa
     6001 acctgacgag tttctcgccg aggctttcgt cggcgatgcc ccgtcggccc aggcgttgtg
     6061 gatcaaagca gagctcaagc acgagctttc tcaggtgctc gctcacccgc ccgggctgcg
     6121 cgtacgctac tggcaggcgg gatcccggac tgcctggatc ctggatgaga tcggcaagga
     6181 cctgcccatc acggccggga tcgtgatcaa cgacggcgcc atcgaggacg tacgggtcct
     6241 cgagtttcgc gaaagccggg gctgggaaat ccgctatccg ttcttcacgc ggcagttcca
     6301 gaatgcccgg ctccagggcg atcgcctgac ccagaacatc gacggtatta ccggcgccac
     6361 cctctccgtc cgggccatga agcgtatggc cacggccgcg cttctgcttc atgagcattc
     6421 cagcgccagc agcaccacgc tcgccaaggc accctagacg ggtcaatcaa tggcacagaa
     6481 ccgcgggcat ctgcgcggtg ctcgtgctcg tctacctgct cgctaccggg gtgccgcttc
     6541 aatacgccgc agagctggac ctcgggcaac gtcacgttgc cagcgagtgg gtgctcgatt
     6601 ggtacgggct acaggcgccc gatgaggcaa cacgcagcgg cccggccgtg atgctcggcg
     6661 gaaccctgtt cctggacgga aacgagcagg ctgggctcga acgcctggtg ggcgcgatac
     6721 cggcgggcgg gattctggcc gtggcgagtt cagacgaggt ctggcttttc caccccgggg
     6781 tacccgaccc ggaaagaacg gcattccggc ccaacattag gcgcatcggc agcctggacg
     6841 gtcagatcta cctcgacacc accgagggtc tgctggcagc agacggtctg ctgatgaact
     6901 ggaccccgac gaacgcgccc gccggcgcca tcgagtgggg acgcgtgacg cccctgagcg
     6961 ctggggaggc agcggcctac cggcagcgat tccggggcag gatgctgacc gcggagcgct
     7021 ggctgcagga cctgcacagc gggcggttct tcggcacgcc gggcgtgatc atcgttgacg
     7081 tggcgtcgct gctgatgctg atcctggcag gaaccgggat cgtgctgtgg tggcgttcga
     7141 gacgcggctg agagccgtgc cggaccggcc gacggtcact cgacgtcgag caactccctg
     7201 tactggcgca tgtgcctgag cgcagacacc tcgtcaccct gcatctggta gatgcgggag
     7261 aggttgaaat gcgcatccgg cacgccgggc gcacgccggt agtactcggc cgccttatcc
     7321 atgtcgtcga gctcgtcgta aacggttccg agattgtagt aggcgagctg atgattcgag
     7381 accgcgacga gggcaagctc gtagtgtcgc ttggcgaact tgagattgcc cttgagctgc
     7441 atgagccgcc cgaggttcac gtgggcgtcg gcgttcttgg gattgagctc aatggcccgg
     7501 gagtatgcgt ctggagcgcg gtccggatcg acctcctcga ggtcgaggcc caggttgtac
     7561 cactcgtcgc tgtccagacc gtcgctttcg cgcaggacga tgaggttgcg ctgggccagg
     7621 gtcgccacgt tgtccgccag ctcccgcacg gtgaagtcca gctgcccctg ccccgtttcc
     7681 acgtcccaga cctggttgtc ttcccggacc accacgttgt tgccatcggc gaagatgcgc
     7741 accgctgcca gcgatttgac ctggctgagc tc
//
LOCUS       DBNHQR010000107         7946 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_833154, whole genome shotgun sequence.
ACCESSION   DBNHQR010000107 DBNHQR010000000
VERSION     DBNHQR010000107.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7946)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7946)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7946
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_833154"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="AC16VR_02375"
     CDS             <1..744
                     /locus_tag="AC16VR_02375"
                     /inference="COORDINATES: protein motif:HMM:NF019843.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6010028.1"
                     /translation="SADAMYALGVLYMNEHYGAENRDSGVPLLRQAAELEHSDAAMLL
                     AHMHYTGEGVERDLDRATDYYVASAESGNDFARRSYARFLLAEAREDPRALQWLEELA
                     EDDDAEAMILLGNLHARGVGVKQSLRRAVGWYKDAVDASGGDANIVNEVAWTLTVSDL
                     EGLKRTRYALNIMEDLMTRDDEARRKPEYLDTWAATYAANGDFIRAVAVQEEAVEAAE
                     DDQYEGVREIIKTHLDAFKSGKTITETAP"
     gene            741..1772
                     /gene="mnmH"
                     /locus_tag="AC16VR_02380"
     CDS             741..1772
                     /gene="mnmH"
                     /locus_tag="AC16VR_02380"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008751.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HIR6010029.1"
                     /translation="MNGTDPETLLAEGARFIDVRAPSEFTRGAVPGAINLPILDDDER
                     HEVGSTYNNEGHNAAVALGYRLVSGAVRQARVAAWAEALDAYPEAWIYCWRGGQRSQI
                     AQEWLHEAGYEVPRVEGGFKALRQCCLEVLARAPHALPWLVLAGRTGSGKTVLIRERP
                     DSIDLEGQANHRGSAFGGRSCTQPSPISFENALAVAFLDLDSQRLIVEDESRTIGRLA
                     LPAAWHERMQTAPVVVVEADLSERVENIRREYVDEPLAEGVTPGDLQARYQGALERIS
                     RRLGGDRCRAVARALAEGFASGHHHAWIEQLLLWYYDPMYDYQLSKKQDRIVMQGDRD
                     AVREFLATP"
     gene            complement(1811..4432)
                     /locus_tag="AC16VR_02385"
     CDS             complement(1811..4432)
                     /locus_tag="AC16VR_02385"
                     /inference="COORDINATES: protein motif:HMM:NF036578.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HIR6010030.1"
                     /translation="MLGAPTRLRCEYLSNPLGIDETRPRLSWWVDDDRPAELQTAYRI
                     LAASSPQRLAAEEGDLWDSGRVASHQTLNVEYHGGPLTTGQRVWWSVRSFDSDGLPSP
                     WSEPAFFEVGPCGQEDWSARWIGASLMGGAATSVQVPVFRRGFELPEAALAARLHVAA
                     LGVCAVEINGQRVGRDELTPGWTDFRKRVRYRTYDVTDHLQTGENALGALLGDGWYCG
                     HPGAGQRQQYGDRPLLLAQLNITLASGATIAIGTDEAWKWRPSWLLQADVLGGESVDA
                     RQRIGDWSSPGCADDGWDPAQVHDLPDLTVCASMHPPIRAQGVLEPVADAERRDDPVR
                     PPRWIFDFGQNLLGRVLVSVKAAAGSGVRVRYAESLGPTDDFGDRAGDGVDWYTASGV
                     PGGETFEPTFSVHGFRYVEVCGDLADDEPGVVAVAVNTDLDATATFESDHPLLDRLHA
                     NIVANQRGCALDVPNAGFSLERRIPLTGEARAFLGTAAMNLDVAAFLAKWLTDLADAQ
                     LPDGGFPSVAPVPPLVPALQQDGGPGWSDAFAACAWILYRHFGDRRVLERNYPALRTY
                     VLGQAAAWPDCLARPGSHPLGPADRGAVASLKATALFLQAVRLASRIAGVLGNLSDFE
                     QFDGLAQSIRSAFRNRFVTHDGLVVGDCVTSYTLALHLGLLERGERRAAFEELVAKLE
                     DGEPPAVEPLAAPYLLGVLTRGGRVDLAYLVLLGSRPGSWLYPVLHGASSLWDGETES
                     SRLARGSVGEWLYTGLAGLDLDDDLSETNNAFRRIRIQPHPPLRDGIELAGNVPPMRR
                     VKASLDTVHGRYESAWEILEDGFVLRVRVPGNCSALVVMPDGTEHEVVAGRHEFKQDF
                     LGETDEIPILREIAEAS"
     gene            complement(4541..5380)
                     /locus_tag="AC16VR_02390"
     CDS             complement(4541..5380)
                     /locus_tag="AC16VR_02390"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR6010031.1"
                     /translation="MVNWEDLRYFDAVARLGSVRRAASALGVNPSTVTRRVEQLERHL
                     GVRLFTRSRRGLELTADAAVAAGTLRELADRLRSVEDQLGQQTGALGGEVRCGIPDYL
                     PAGWWMPEIARFTGEHPELMVAFMESNRLPDLASGEADLAVNARKDPPEALIARPLGQ
                     LVFAAYRHLDCHDEAWLGSALQAAIAPDYPGGARVTAILPSVSLQVAAVEAGMGSSLL
                     PCAAVDDRPGFVRDDSRPEVIRPLWLLLHPDSRPLARVQTVADALTDALRARLARSAG
                     NER"
     gene            5801..6352
                     /locus_tag="AC16VR_02395"
     CDS             5801..6352
                     /locus_tag="AC16VR_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010032.1"
                     /translation="MEFIGAFSGLERIAIIAAAMLIAYLGFRLFANARTPGLVFMGIA
                     CAVLFGALFTTGSHVEKLGASYQVAAVSPAAEAPAAAQPAEPAADVPAQVMAESAEAV
                     SLAPTTGPGEVAAAPAQSEPIAEPAQAAVVLEEAVAAAPEPAAQSDEALEEPATTILS
                     SEELGGRIMSVRSENVTLEWSRE"
     gene            complement(6367..7191)
                     /locus_tag="AC16VR_02400"
     CDS             complement(6367..7191)
                     /locus_tag="AC16VR_02400"
                     /inference="COORDINATES: protein motif:HMM:NF012575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HIR6010033.1"
                     /translation="MPEHETLPPLASGRIPHVGTYRRVLPVSLERMYENALDWEHLPY
                     VHASSFAAIRCLDAGQWGWRAELTGAGGEASIIELLLDRRCHRWITRNLEGPNAGAEI
                     WTHVFALAPVAGAERIEIVVDFFVPDVPEAGRARVGQAYALLYATLYDEDVAMMVERQ
                     AQLDRRVEGSRPDERRLALGPREVLDLPLRCTVAGRDYVVAEDAGSLVAYPAICPHAL
                     GPLARGPLGQGVVTCPWHGYRFDVRSGECLSGHSCRLSRLPVISEESGTVVLTIPD"
     gene            7527..>7946
                     /locus_tag="AC16VR_02405"
     CDS             7527..>7946
                     /locus_tag="AC16VR_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010034.1"
                     /translation="MTATLICRGLFLSLLILAGCQPGDSAGEGSEPATVIDDAAALPA
                     GNSVAPLLEGIGPVDFPISTTSERAQAYFNQALTFSYGFNHAEAARSFREAARLDDTC
                     GICWWGVALAHGPNINKEMTSEDVPVAWEALRNALARQ"
BASE COUNT         1429 a         2739 c         2566 g         1212 t
ORIGIN      
        1 tcggccgacg ccatgtacgc gctgggcgtt ctctacatga acgagcacta cggagccgag
       61 aaccgtgact ccggggtgcc gctgctgcgc caggcagcgg agctggaaca cagcgacgcc
      121 gccatgctgc tcgcgcacat gcactacacc ggcgagggcg tcgagcgcga tctggaccgc
      181 gcgacggact actacgtcgc ttcagccgag agcggcaacg acttcgctcg tcgcagctac
      241 gcgcgtttcc tgctggccga ggcgcgcgag gatcccaggg ccctgcagtg gctggaggaa
      301 cttgctgagg atgacgatgc ggaggcgatg atcctgctgg gcaacctcca cgcacgcggc
      361 gtcggggtaa aacagagcct gcggcgcgcg gtcggatggt acaaggacgc cgtggacgcg
      421 tccgggggcg atgcgaacat cgtgaacgag gtggcatgga cgctgacggt cagcgacctg
      481 gagggcctga agcgaacacg ctacgcgctc aacatcatgg aagatctcat gacccgcgac
      541 gacgaggcgc gccgcaagcc ggagtacctg gacacctggg cggcaaccta tgcggccaac
      601 ggcgatttca tccgcgccgt ggcggttcag gaggaagccg tcgaggcggc agaggacgac
      661 caatacgagg gcgtgcggga gatcatcaag acccatctgg atgccttcaa gagcggaaag
      721 acgatcaccg aaaccgcgcc gtgaacggaa ccgatccgga aacgctgctg gctgaaggcg
      781 cgcgcttcat cgacgtcagg gcgccctccg agttcacccg gggcgccgta cccggcgcca
      841 tcaacctgcc gatcctggat gacgacgagc gccacgaagt cggctccacc tacaacaacg
      901 agggtcacaa cgctgccgtc gccctggggt accgcctggt gagcggcgcc gtgaggcaag
      961 cccgcgtagc cgcctgggct gaggccctag acgcgtaccc cgaggcgtgg atctactgct
     1021 ggcgtggggg ccagcgctcc cagatcgcac aggagtggct gcacgaagcg ggctacgaag
     1081 tcccccgggt cgaaggcggc ttcaaagctc tgcggcaatg ctgcctggag gtgctggcca
     1141 gggcgccgca cgcgctgccc tggctggtcc tcgccgggcg gaccggctcg ggcaaaaccg
     1201 tgctgattcg tgagcggccg gacagcatcg atctggaagg ccaagccaac catcgcggct
     1261 ccgcgttcgg cggcagatcg tgcacgcaac catccccgat cagcttcgag aacgctctgg
     1321 cggtggcctt cctcgatctc gactcccagc gattgatcgt ggaggacgaa agccggacca
     1381 tcggccgcct cgccctgcca gccgcttggc atgagcgcat gcagaccgcg cccgtggtgg
     1441 tggtggaagc cgacctgtcc gagcgggtgg agaacatccg ccgcgagtac gttgacgaac
     1501 cgcttgccga gggcgtgacg cccggcgacc tgcaggcccg ctaccaggga gcgcttgagc
     1561 gcatcagccg ccgcctcggc ggcgaccgct gtcgggccgt ggcgcgcgcc ctcgcggagg
     1621 gcttcgcctc cggccaccac cacgcctgga tcgagcagct gctgctgtgg tactacgacc
     1681 ccatgtacga ctaccagctg agcaagaagc aggaccgcat cgtcatgcag ggcgaccgcg
     1741 acgcggtccg ggagtttctc gccacaccct gaccggctgc cccacggcgg cccaaccccg
     1801 ccgcagggaa tcaagacgcc tcggcaatct cccggagaat ggggatctcg tcagtctcgc
     1861 caaggaaatc ctgcttgaac tcgtgacgtc ccgccaccac ctcgtgctcg gtcccgtccg
     1921 gcatgacgac cagcgccgag caattgcccg ggactcggac gcggagcacg aacccgtcct
     1981 cgaggatctc ccaggccgac tcgtagcgcc cgtgcacggt atccagcgaa gccttgaccc
     2041 gacgcatcgg ggggacgttg ccggccagct ctatcccgtc acgtaacggc gggtgaggtt
     2101 gtatccggat gcgccggaag gcgttgttgg tctccgatag atcatcgtcc agatcgagcc
     2161 cggcaagccc cgtgtacaac cactcgccta ccgagccccg agcaaggcgg ctcgactccg
     2221 tttccccatc ccacagcgag cttgcaccgt gcagcacggg atacagccag ctgcccgggc
     2281 gcgagccgag cagcaccaga taggccagat cgacgcggcc gccccgcgtc agcaccccaa
     2341 ggagataggg tgcggccagc ggttccactg caggcggctc gccatcttcc agcttcgcca
     2401 ccagctcctc gaacgccgcg cgccgctcgc ccctttccag aagccctaga tgcagagcaa
     2461 gggtatagct ggtgacgcag tcgccgacca ccaggccgtc gtgggtcacg aagcggttcc
     2521 tgaaggcgga acggatactc tgcgctaagc cgtcgaactg ctcgaagtcg ctcagattcc
     2581 ccagcacccc ggcaatgcgc gaggcaagcc gaaccgcctg aagaaacaaa gccgtcgcct
     2641 tcagcgaggc aacggcacct cggtcggcag gcccgagcgg atggctgccc ggcctggcca
     2701 gacagtccgg ccaggccgcc gcctgcccaa gcacataggt ccgcagcgcc ggatagttgc
     2761 gttcgagaac ccgccgatct ccgaaatgcc ggtagagaat ccaggcacat gccgcaaacg
     2821 cgtccgacca gcccgggccc ccgtcctgct gcagcgcggg caccagcgga ggcacaggag
     2881 cgacgctggg gaacccccca tccggcagct gcgcatctgc cagatccgtc agccacttcg
     2941 ccagaaaggc cgccacgtcg agattcatgg ccgcggtgcc gagaaaggca cgcgcctccc
     3001 ccgtcagcgg aatcctgcgt tccaaggaga agcccgcatt gggtacgtcc agggcgcagc
     3061 cgcgctggtt ggcaacgatg ttggcgtgca ggcgatccag caggggatgg tccgactcga
     3121 acgtggccgt ggcgtccagg tcggtattga cggctaccgc aaccactccc ggctcgtcgt
     3181 ctgccagatc gccgcaaacc tctacatacc ggaagccgtg tacgctgaaa gtcggttcga
     3241 aggtctctcc gccgggcacg ccgctggccg tgtaccaatc gacgccgtct cctgcccggt
     3301 cgccgaagtc atccgtcggg cccaggcttt ccgcgtatcg cacccgtacg ccagagcccg
     3361 cagccgcctt cacgctgacc agcacccggc cgagcaggtt ctgcccgaaa tcgaatatcc
     3421 agcgcggcgg ccgaaccggg tcgtcacggc gctcagcatc ggccacgggc tccaggaccc
     3481 cctgggcacg gatggggggg tgcatgctgg cgcacacggt gaggtccggc agatcgtgca
     3541 cctgggccgg atcccagccg tcatccgcgc agcccggtga gctccagtcc ccgatgcgct
     3601 gacgggcgtc gacgctttcg ccgccgagca cgtccgcctg cagcaaccag gacgggcgcc
     3661 atttccaggc ttcgtcggtg ccgatggcga tcgtcgcccc ggaggccagg gtgatgttca
     3721 gctgggccag cagcagcggt cggtcaccgt attgctgccg ttgcccggcc ccggggtgcc
     3781 cgcagtacca gccgtccccc agcagcgcgc caagggcatt ttccccagtc tggagatggt
     3841 cggtcacgtc gtaagtgcgg tagcgaaccc gtttgcggaa atccgtccag cccggcgtga
     3901 gctcgtcacg tccgacacgt tgcccgttga tctcgacggc acagacgccg agggcggcca
     3961 cgtgcaaccg agcggcgagc gctgcctcgg gaagctcgaa accgcgcctg aagacgggaa
     4021 cctgaacgct ggtggccgcg ccgcccatga gcgaggcgcc gatccaacga gcgctccagt
     4081 cctcctgccc gcaggggccg acctcgaaga aagcgggttc gctccagggg ctcggcagac
     4141 cgtcgctgtc gaacgagcgc accgaccacc acacgcgctg accagtggta agcgggccgc
     4201 cgtggtactc cacgttcagg gtctgatggc tggccacccg acccgagtcc caaagatcgc
     4261 cctcttccgc agctagccgc tgcgggctgc tcgcggcaag aatacggtac gcggtctgga
     4321 gttcggcggg ccggtcgtcg tccacccacc aggaaaggcg gggtcgggtc tcgtcaatgc
     4381 caagcggatt ggaaaggtac tcgcagcgaa gcctcgtcgg cgcgcctagc attgcttctc
     4441 cttcagcaga ggaaacgcgt ccctgacggg cggccattct gcgctgcgcg gccgagccgc
     4501 gcaagccggg gtgcccgaac ggatgaaagg ccatccgtga tcatcgctca ttgccggcag
     4561 atcgggccaa gcgggcgcgg agggcatcgg tgagcgcatc ggcgacggtc tgaacccggg
     4621 caaggggtct cgagtccggg tgcaacagca gccacagcgg cctgatcact tcgggccggc
     4681 tatcgtcgcg gacgaacccc gggcggtcat cgaccgcagc gcaaggcagc aggctgctgc
     4741 ccatgcccgc ctccacggca gccacctgca gcgacacgga cggcaggatc gcggtgaccc
     4801 gagcaccacc ggggtaatcg ggcgcaatgg ctgcttggag tgccgagccg agccaggcct
     4861 cgtcatggca gtcgaggtga cggtaggctg caaacaccag ttggccaagc ggccgggcaa
     4921 tgagggcctc cgggggatcc ttccgggcat tcaccgccag gtcggcttcc ccggacgcga
     4981 ggtccggcag tcggttggac tccatgaacg ccaccatgag ctcgggatgt tccccggtga
     5041 acctcgctat ctccggcatc caccagcccg ccggcaggta gtcgggaatg ccgcaacgga
     5101 cctcaccccc cagggccccg gtttgctgcc cgagctgatc ctcgacggat cgaagccgat
     5161 cggccaattc tctgagggtt ccggcagcaa cggcggcgtc ggcggtcagc tccagtccac
     5221 gacgtgagcg ggtgaacagg cgcacaccca gatgcctttc aagctgctcc acccgtctgg
     5281 tgaccgtgga cgggttcacg ccaagcgccg acgcagcccg gcgaaccgat cccaaccggg
     5341 ccacggcatc gaagtatctg aggtcttccc agttcaccat gggggatcct ttggatttct
     5401 cgcccgccag cgttgcgatt atgcaacatc atgttgcgtt acccaaggtt gcattctgtg
     5461 ttaccgaaag taacataggt tcgacgattc gctgaggggt acccgaaatc cctcccctcc
     5521 cctcccttcg ggtatccctc cgtttcgacc cctccctgtc ttaaacggcg ggtgcagtga
     5581 ctaaccaagc gctgacccgc cgtctttttt ttccccttct tcctgctcca gcgatcgagg
     5641 ttggttcgga tacggcagac cgactcgccg tgagtggcgt atgcccctgg gaccgacggg
     5701 gttgtgatca aaagacgaat acatatagac aaataggctg tctatatagc aacatcccgg
     5761 tttggaccaa taacaactgc atcaccgttg ggggttaacc atggaattca tcggcgcatt
     5821 ttccggtctg gagcggatcg ccatcatcgc cgccgcgatg ctgatcgcgt atctgggctt
     5881 tcggctcttc gctaacgcgc gcacgcccgg actcgtcttc atgggcattg cctgtgccgt
     5941 tctgttcggc gcgctgttca cgacgggcag ccatgtcgag aaactgggcg cgtcctatca
     6001 ggtcgccgcg gtgagcccgg cggcagaagc gccggccgct gcacagcccg cagagcccgc
     6061 ggccgacgtc ccggcacagg tcatggccga gtccgcagag gcggtttcgc tggcgccgac
     6121 cacgggccca ggcgaagtag cggctgcgcc ggcccaaagc gagccgatcg cagagccggc
     6181 acaggcagcc gtcgtcctcg aagaggccgt cgcggcggcc ccggagcctg ccgcacaatc
     6241 cgacgaggca ctcgaggagc ccgccacgac catcctcagc agcgaagagc tcggcggccg
     6301 catcatgtcg gttcgctccg agaacgtcac gctggagtgg tcgcgcgagt aaccgcgtcg
     6361 ccgaagtcag tcgggtatgg tcaagaccac cgtgcccgac tcttccgaga tcaccggcag
     6421 acggctcaag cggcaggaat ggccgctgag acactcaccg ctgcgcacgt cgaaccggta
     6481 accgtgccag gggcaggtca ccacgccctg ccctaacggg cccctggcca gagggccgag
     6541 cgcatggggg caaattgccg ggtaagccac gagagacccc gcgtcctccg ccaccacgta
     6601 atcgcgcccg gccacggtgc agcgcagtgg caggtcgagc acttcccggg gcccgagagc
     6661 gagccggcgc tcgtccgggc gcgatccttc cacccgccga tctagctggg cctggcgctc
     6721 caccatcatg gcgacgtctt cgtcatagag cgtggcatac agcaaggcgt acgcctggcc
     6781 aacccttgcc ctgcccgcct ctggcacgtc gggcacgaag aagtccacga cgatctcgat
     6841 gcgctcggcc cccgcaaccg gcgccagcgc gaacacgtgg gtccagatct cggcgcccgc
     6901 attggggccc tccaggttgc gcgtgatcca gcgatggcat cggcgatcca gaagcagctc
     6961 gatgatcgac gcctcgcccc cggcaccggt aagctcggcg cgccagcccc attggccggc
     7021 gtcaaggcag cgtatggcag caaagcttga ggcgtgcacg tagggcaggt gctcccaatc
     7081 cagggcgttc tcgtacatcc gctcgaggct gaccggcagg acgcggcgat aggttccaac
     7141 gtgagggatc cgccccgatg cgagcggcgg cagggtctcg tgctccggca tgcaaagtcc
     7201 cgtggattcg tcccgtcgac agcgtagcaa agcccctcgc ccggggcatt gcaagcagga
     7261 ttcattgcgc agtgcacgat tgaccgcaac gaacgcttct gctctcatcg gcgcggggag
     7321 gaaagggaga gtcatcgtgc ttctctcttt cagagggcgg ccgaactcgc atgagttcgg
     7381 ccgacttcgt ttcaagcccc gacttttccc gtttcttccg gcagcgtggc gtcgtcgagc
     7441 gcatgcggca gggcgctgcc acagcctgga cccggtcgcg gccccaacgt agagtcgttc
     7501 gtccggttgt cgcagaggaa gtcaccatga ccgcaacgct catctgcagg ggcctgttcc
     7561 tcagcctgct gattctcgcc ggctgccagc ccggggactc ggcgggcgaa ggttccgaac
     7621 cggccaccgt gatcgatgat gcagctgcgc tgcccgccgg gaacagcgtg gcacccctgc
     7681 tcgaaggcat cggacccgtc gactttccca tcagtaccac ctcggagcgc gcccaggcct
     7741 atttcaacca ggcgctcacg ttcagctacg gtttcaacca cgcagaagcg gcccgctcgt
     7801 tccgggaagc cgcccggctc gacgacacct gcgggatctg ctggtggggc gtcgcgctgg
     7861 cccacggacc caacatcaac aaggaaatga catccgaaga cgtgcccgta gcctgggaag
     7921 cgctgcgcaa cgccctggcg cgccag
//
LOCUS       DBNHQR010000108         5933 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_833223, whole genome shotgun sequence.
ACCESSION   DBNHQR010000108 DBNHQR010000000
VERSION     DBNHQR010000108.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5933)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5933)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5933
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_833223"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2049
                     /locus_tag="AC16VR_02410"
     CDS             <1..2049
                     /locus_tag="AC16VR_02410"
                     /inference="COORDINATES: protein motif:HMM:NF014026.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HIR6010035.1"
                     /translation="GELAAALIDGVQSQGVGTSAKHFAVNNQEHGRMTVDAIVDERTL
                     REIYLRGFEIAVRKSQPWTVMCAYNRLNGTYCSEHAWLLDSVLRDEWGFAGLVVSDWG
                     AANERARGVAAGLDLEMPASGGINDARVAAAVRSGELAEAVLDRAATRNASLALLGGE
                     LEGRPRDVDHDAHHGLARRAAAQSAVLLKNDGGLLPLGRDVSIAVIGAFAKRPRYQGA
                     GSSQVRPTRLDCAWDAMEKIAEAGRLTFAAGYDPRRSEPDQALIDEAVDLARAAEVAV
                     VFAGLPGIYESEGFDRAHMRLPDQHNRLIAAVCDANPSTVVVLSNGAPVEMPWIDRPA
                     AVLEAYLGGQAGGSAAADLLFGLANPCGKLAETFPMRQADVPSDAWFPGSDRQVQYRE
                     GLYVGYRYFDTFDRGVLFPFGHGLSYTRFEYGEIAMDDLRYEQGGRLEVSVDVTNVGD
                     RPGAETAQLYVAALTSGAYRPFHELKGFAKTRLEPGETRRVTFVLDDGAFAVYDPEAK
                     AWIVEAGEFELRVGASSRDIRQTRIITVVSDQTLSPATEAVGGPNAGSPNAGDPGSAD
                     AGLAVADAAFAAMLGRPVPDPEPVRPYHLNSSVNEIAESWLGRLVKARVVAGFQQRMG
                     ARSSDETVQKMFEEMANNMPLRALPLFSRGAISFAQLEILVALLNGRVLRALRLWLSG
                     "
     gene            2053..3591
                     /locus_tag="AC16VR_02415"
     CDS             2053..3591
                     /locus_tag="AC16VR_02415"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase family protein"
                     /protein_id="HIR6010036.1"
                     /translation="MRVLLLRALIASLCAGLAACQDPASGVGPTTGDLEPGRSDRPPN
                     VIILFADDLGYGDLGSYGHPTIRTPRLDALAAAGQRWTDFYAAAPVCSPSRGALLTGR
                     LPNRTGLYGRRLNVLFPNDPGGLPTAERTLAEALGERGYATAVVGKWHLGDRPEAYPT
                     RHGFDYWFGLPYSNDMDWVDAMSFEEVLALAAAGRMPEVEEEIARRQALYLDPRNEYW
                     NVPLVRSQRLATGITDEVVERPALQASLTSRYTDEAVAFIRRHQAQPFFLYLPYTMPH
                     TPLFPGDAFAGGSRGGRYGDVVEELDWSAGAIADALQELGLAENTLVVFTSDNGPWLA
                     MDHHGGSSGPLRDGKGTTFEGGVRVPAIFSWPGRIKPGVTVGIGSILDLYATALALAG
                     ADAGAAGDIDGFDLSGTLLEGRPSPRSSIAYYRSGELMAFRQGEYKLHLVTQGAYGRP
                     PERVVHEQPLLYHLGEDPGERFDAAGRHPDVVKRLQRAVAAHRASLVEQPPLFDRRLQ
                     PVHD"
     gene            3614..4399
                     /locus_tag="AC16VR_02420"
     CDS             3614..4399
                     /locus_tag="AC16VR_02420"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6010037.1"
                     /translation="MTDGRLAGRTALVTGASRGIGRAIALAYGREGANVFLTATRLDN
                     LSDTRDRLADMGVEVACHAADVGKPDELETLFAAAAEWRDGVDVLVNNAGIYIGKPFE
                     AYSLEEFDRLMKINVYSVFQLMQLAIRHMTARGRGKIVNIASTAGKWESPNQAAYNTS
                     KHAVVGMTRCAALENASRGLNINAICPGLVETDMLGEFKTHADAQGISFDEFKRAVAA
                     QRIPMGRFLAPEEVAHIAVYLGSGESDGMTGQTITISGGMRMG"
     gene            4443..>5933
                     /locus_tag="AC16VR_02425"
     CDS             4443..>5933
                     /locus_tag="AC16VR_02425"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HIR6010038.1"
                     /translation="MAVQLLADITREHAKDRPDRRAMRYDEDGREWTYAELDADANRV
                     ANALLGDGVEPHGRIAYLDKNAPEYFLYLFGGAKINAVSVAVNWRLAAREMEYILNNS
                     EAGVLVIGDEFLGHLAQMDLPAVTRVVVLGDPGDTGHITFDQWIAGQPDDDPALPAAP
                     GDTCYQLYTSGTTGPPKGVELSHANFVHCVDQALAPVGIRSESVSLVCMPLFHIAGSG
                     FGVLGMYWGADMVLLRDVDLAAIVRVVPEQRVTHAMFVPAVLQFLLAQPGVRDADLSS
                     FETILYGASPITEAVLVEAMDVFGCDFLQGYGLTETTGGVSLLAAEDHDPGGPRAHLL
                     RSCGKVIVNHEVRIVDPESRREMPDGEVGEIWVRGPQVMKGYWKNPEASAESLVEDGW
                     FRTGDAGYLLDGYVFIHDRVKDMIISGGENIYPAEIENVLMAHEGIADAAVIGVPSER
                     WGETVKALVTRSDADLTEQQIIEYCRAHLAGYKCPTSVDWIAEIPRN"
BASE COUNT          958 a         1919 c         2086 g          970 t
ORIGIN      
        1 ggggagctgg ccgccgcgct gatcgacggc gtgcagtccc agggggtcgg caccagcgcg
       61 aagcactttg ccgtcaacaa tcaggaacac ggacgcatga ccgtggacgc catcgtcgac
      121 gagcgcacgc tgcgggagat ctacctgcgg ggattcgaga tcgccgtgcg caagtcccag
      181 ccctggacgg tgatgtgtgc ctacaaccgt ctcaacggga cctactgcag cgagcacgcc
      241 tggctgctcg attcggtgct gcgcgacgag tggggcttcg cgggcctcgt ggtcagcgac
      301 tggggtgctg ccaacgagcg ggcccggggg gtggctgccg ggctggacct ggagatgccg
      361 gccagcggcg ggatcaacga tgcccgggtg gcagccgccg tgcgctcggg cgagttggcc
      421 gaggccgtgc tcgaccgggc ggcaacccgc aatgcgagcc tggccctgct cggcggcgag
      481 ctggaaggtc ggccccggga cgtcgaccac gatgcccacc atggcctggc gcgccgggcg
      541 gcggcgcaga gcgccgtgct gctgaagaac gacggcgggc tgctgcccct tggcagggat
      601 gtatccatcg ccgtcatcgg cgcgtttgcc aaacgcccga gataccaggg cgcgggcagt
      661 tcccaggtgc ggccgacccg cctggattgc gcctgggacg ccatggagaa aattgccgag
      721 gcgggccggc ttaccttcgc ggccggctac gatccgcgcc gcagcgagcc cgatcaggct
      781 ctcatcgacg aggccgtcga cctggcccgc gccgccgagg tggcggtggt gtttgccggc
      841 ctgccgggca tctacgaatc cgaaggtttc gatcgtgccc acatgcgcct gcccgaccag
      901 cacaaccgtc tgatcgcagc cgtctgcgat gccaacccca gtacggtggt cgtgctgagc
      961 aacggcgcgc ccgttgagat gccctggatc gatcgcccgg cggcggtgct cgaagcgtat
     1021 ctcggcggtc aggcgggcgg ttccgccgcg gccgacctgc tgttcgggct ggcgaatccc
     1081 tgcggcaagc tggcggagac cttcccgatg cgccaggccg acgtgccctc cgatgcgtgg
     1141 tttccgggct ctgatcgcca ggtgcagtat cgtgagggtc tatacgtcgg ttaccgctac
     1201 ttcgacacct tcgaccgggg ggtgctcttc ccgttcggtc acgggctcag ctacacccgc
     1261 ttcgagtatg gcgagatcgc catggacgat ctgcgctacg agcagggcgg caggctagag
     1321 gtgagcgtcg acgtgacgaa cgtcggcgac cgccccggcg ccgagaccgc gcagctctac
     1381 gtcgccgctc tgacctcggg cgcctaccgg ccgttccatg agctgaaggg gtttgccaag
     1441 acgcggctgg agccgggcga gacccggcgg gtgacgttcg ttctcgatga tggcgccttc
     1501 gccgtctacg atcccgaggc caaagcctgg atcgtggaag cgggtgagtt cgagctgcgc
     1561 gtgggcgcat cgagtcgcga catccgccag acccgcataa tcacagtggt ttccgatcag
     1621 accctgtcgc cggcgaccga agccgtcggg ggcccgaacg ccggtagccc gaacgccggg
     1681 gacccgggca gcgccgacgc aggcctggcg gtggcggacg cggcgtttgc cgccatgctc
     1741 gggcgaccgg ttccggatcc cgagccggtg cgcccctacc acctgaactc gtcggtgaac
     1801 gagattgccg agagctggtt gggccgtctg gtgaaggcgc gggtggtggc cggcttccag
     1861 cagcgcatgg gcgcgcgcag cagcgacgag accgtgcaga agatgttcga ggagatggcc
     1921 aacaacatgc cgcttagggc cctgccgctg ttctcgcgag gggcgatcag ctttgcgcag
     1981 ctggagattc tggtggcgct gctgaacggg cgggtgctgc gcgcgctgcg gctgtggttg
     2041 agcggctgag gcgtgcgcgt cctgctcttg cgtgccctga tcgcgtcgct gtgtgcgggc
     2101 ctggcagcct gtcaggatcc ggcgagcggg gtggggccca cgaccggtga tctcgagccc
     2161 ggccggagcg accggccccc gaacgtgatc attctctttg ccgacgacct cggctacgga
     2221 gatctcggca gctacggtca cccgaccata cggacgcccc ggctggacgc gctggccgct
     2281 gccgggcagc gctggacgga tttttacgcg gccgcccccg tctgctcgcc cagccggggc
     2341 gcgctgctga ccggcaggct gcccaaccgg acggggcttt acgggcgccg gctcaacgtg
     2401 ctgtttccga acgaccccgg cgggttgccg acagcggagc gcaccctcgc ggaagccctg
     2461 ggtgagcggg gctacgcgac ggccgtggtc ggcaagtggc acctcggaga ccggcccgag
     2521 gcttacccca cccgccacgg gttcgattac tggttcgggc tgccgtactc caacgacatg
     2581 gactgggtcg acgccatgtc cttcgaggaa gtgctcgcgc tggccgcggc gggtcggatg
     2641 cccgaggtcg aagaggagat cgcgcgccgg caggcgctct atctcgatcc acgcaacgag
     2701 tactggaatg tgccgctggt gcgcagccaa cgattggcga cgggcatcac cgacgaggtc
     2761 gtcgagcggc cggcgctgca ggcgtcgttg acaagccgct acacggacga agccgtggcc
     2821 ttcatccggc gtcatcaggc gcagccgttc ttcctctacc tgccgtacac gatgccccac
     2881 acgcctctgt tcccgggcga cgcttttgcc ggcgggagcc ggggcggtcg ttacggcgac
     2941 gtggttgagg agctggactg gagcgcgggc gcgatcgcag atgctctgca agagctgggt
     3001 cttgcagaaa acaccctcgt ggtgttcacc agcgacaacg ggccgtggct tgccatggat
     3061 catcacggcg ggtcttcggg tccgctgcgg gacggcaagg gcaccacctt cgagggcggt
     3121 gtgcgggtgc cggcgatctt ctcgtggccg ggccgcataa agccgggggt gaccgtcggt
     3181 atcggctcga tcctggatct ctacgcgacg gcactggccc tggctggtgc tgacgccggc
     3241 gccgccggcg acatcgacgg cttcgatctc agcgggacgc tgctggaagg ccggccgagc
     3301 ccgcgctcga gtatcgccta ctatcgcagc ggcgagctca tggccttccg ccagggcgag
     3361 tacaagctgc atctggttac ccagggcgcc tacggccggc cgcccgagcg cgtggtccac
     3421 gagcagcccc tgctttacca cctgggggag gatccgggcg agcggttcga tgcggcgggt
     3481 cgacatccgg acgtggtgaa gcgacttcag cgcgccgtgg ccgcccaccg ggcaagcctg
     3541 gttgagcagc cgccgctgtt tgatcggcgg cttcagccgg tgcacgattg aacaacgcaa
     3601 ggggaggaat cagatgaccg acggtcggct cgcaggcaga acggcgctcg tcaccggcgc
     3661 cagtcgtggt atcggccgcg cgattgcgct cgcttatggc cgggaaggcg ccaacgtctt
     3721 tctcaccgcc acccgtctcg acaatctctc tgacacccgg gatcgcctgg cggacatggg
     3781 cgtggaagtt gcctgccacg ccgccgacgt gggcaagccc gacgagttgg aaacgctctt
     3841 tgccgccgcc gccgagtggc gggacggcgt ggacgttctt gtcaacaacg cgggcatcta
     3901 catcggcaag cccttcgagg cctacagcct ggaagagttc gaccggctca tgaagatcaa
     3961 cgtctactcg gtgttccagc tcatgcagct ggccatccgg cacatgacgg ccagggggcg
     4021 cggcaagatc gtcaacatcg cgtccacggc cggcaagtgg gagagtccca accaggccgc
     4081 ctacaacact tccaagcacg cggtggtagg aatgacgcgc tgcgcggcgc tggagaacgc
     4141 ctccaggggg ctcaacatca acgccatctg ccccgggctg gtggagacgg acatgctggg
     4201 tgagttcaaa acccacgcgg acgcccaggg catcagcttc gatgagttca agcgcgcggt
     4261 agccgcccag cggattccca tgggacgctt cctcgccccg gaggaggtgg ctcacattgc
     4321 cgtctatctc ggcagcgggg agtcagacgg catgacgggg cagaccatca ccatatccgg
     4381 cggcatgcgc atgggctgag ccgtcgcgtt cagccggatc aaccggatca agaggaggga
     4441 gcatggcagt tcagctgttg gccgacatca cgcgggagca cgcgaaagac aggcccgatc
     4501 gcagagcgat gcgatacgac gaggacgggc gcgagtggac ctacgcggag ctcgacgccg
     4561 acgcgaaccg cgtggcgaac gcgctcctcg gtgacggcgt ggagcctcac ggccgcatcg
     4621 cctacctgga caagaacgcc cccgagtact ttctctacct gttcggcggg gccaagatca
     4681 acgccgtctc cgtagccgtc aactggcgcc tggcggcccg ggagatggag tacatactca
     4741 acaacagcga agcgggcgtg ctggtcatcg gtgacgagtt cctgggccat ctcgcccaga
     4801 tggacctgcc ggcggtgacc cgggtggtgg tgctgggcga tcccggcgat accggtcaca
     4861 tcacgttcga tcagtggatc gccggtcagc cagatgatga ccccgcgctg cctgccgctc
     4921 ccggggacac ctgctaccag ctctacacct cgggaaccac gggccccccc aagggcgtgg
     4981 agctttccca tgccaacttt gtccactgcg ttgaccaggc cctcgcgccc gtgggtatcc
     5041 gatccgagtc cgtcagcctg gtctgcatgc ccttgttcca catcgccggc agcgggttcg
     5101 gcgtgctggg catgtactgg ggagccgaca tggtgctcct gcgggacgtc gatctggccg
     5161 ccatcgttcg ggtggtcccc gagcaacgtg ttacccacgc gatgtttgtc cccgcggtgc
     5221 tgcagttcct gctcgcccaa cccggggtac gcgatgctga tctgtcgtcg ttcgaaacca
     5281 ttctgtacgg cgcttcgccc atcacggaag ccgtgctggt ggaggccatg gacgtgttcg
     5341 gctgcgactt cttgcagggc tacggcctga ccgaaaccac cggcggcgtg agcctgcttg
     5401 ctgctgaaga ccacgatccg ggtggcccga gggcgcatct gctgcgctcc tgcggaaagg
     5461 tcatcgtcaa tcacgaggta cgcatcgtcg atcccgagag cagaagggag atgcccgatg
     5521 gcgaggtggg cgagatctgg gtgcgcggcc cccaggtgat gaagggctac tggaagaacc
     5581 ccgaggcgag cgccgagagt ctggtcgagg acggttggtt caggacgggg gatgccggct
     5641 atctgctcga cgggtacgtc ttcattcatg accgcgtgaa ggacatgatc atctcgggcg
     5701 gtgagaacat ctaccctgcg gagatcgaga acgtgctgat ggcccacgag gggattgccg
     5761 atgcggccgt gatcggcgtg cccagcgagc ggtggggaga gacggtcaaa gcgctggtga
     5821 cgcgcagcga tgccgacctc accgagcagc agatcatcga gtactgccgt gcccatctgg
     5881 cggggtacaa atgcccgact tccgtggact ggatcgccga gattccccga aac
//
LOCUS       DBNHQR010000109         2028 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1086451, whole genome shotgun sequence.
ACCESSION   DBNHQR010000109 DBNHQR010000000
VERSION     DBNHQR010000109.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1086451"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2011)
                     /locus_tag="AC16VR_02430"
     CDS             complement(<1..2011)
                     /locus_tag="AC16VR_02430"
                     /inference="COORDINATES: protein motif:HMM:NF013123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6010039.1"
                     /translation="MKLTVERLFSDPPLTGSVPAAGQFSPDGRRAAFLRTAEDDRERQ
                     DLWRLDCGNGDLACWLDARQIDDQGSSPTAAEKAERERRRQFQGGISGFAFSPGGDKV
                     LVTAAGAGYLMDTANGRVDRFTPAGTRQTDLRFSPDGRHITYVRDGNLLCWDLAASSE
                     RAITNDGGGTVSYGAAEFIAQEEMHRFDGYWWSPDGTRIAYTRVDESPIPVTNRLEID
                     ADQIRTVEQRYPFTGGANAAVALLVMDWASGAVTEISYLQTPSDYLARVNWAGSELAV
                     QTQSRDQRELRLRFFDPVSGDSRDGLEERSATWINLHDNFRYLEGERFLWTSERDGHS
                     HLYLCDGSRELPLTRGEGRVNRVLHADEHRILFTGWFGNPQEQHLYSVSASAASGPEE
                     PVRLTAEPGWHEVTVDLRGEQFLDRFSAVDRQPSCRVRKLEGPQVNTIGQELADGLSN
                     YAPYLTHHVTPQLGTVVCEDGQTLHYRLTRPSNGSRQKSPVIVHVYGGPGVQRVRNEW
                     APALLQLFVQHGFGVFELDNRGSGNRDRGFEEPIHRQLGKVEVADQVLGADFLRSLDW
                     VDPERIGVFGHSYGGYMTIMCLAQAPASFKAGVSVAPVTDWHLYDTHYTERYLGHPED
                     NASGYGASSVFPHLDGIKGRLLLIHGMADDNVLYTNSTMLYRALQA"
BASE COUNT          345 a          662 c          653 g          368 t
ORIGIN      
        1 gggcctgaag cgcccggtag agcatggtgc tgttcgtgta gagaacgttg tcatcggcca
       61 tcccgtgaat gagcagcagc ctgcctttga tcccgtcgag atggggaaat acgctgctgg
      121 caccgtaccc ggaagcattg tcctcggggt gcccgagata acgttccgtg tagtgagtgt
      181 cgtaaagatg ccagtcggtc accggtgcga ccgaaacgcc ggctttgaac gacgcagggg
      241 cctgggccag gcacatgatg gtcatgtagc ccccgtagct gtgcccgaaa accccaatgc
      301 gttccggatc aacccagtcg agcgagcgaa ggaaatcggc gccgagcacc tggtctgcca
      361 cctcgacctt gcccagctgg cggtgaatgg gctcttcgaa cccgcggtcg cggttcccgg
      421 aaccccggtt gtccagctcg aacacgccga acccgtgctg gacgaaaagc tgcagcagcg
      481 caggcgccca ttcgttacgc acccgctgca cgcctggccc gccgtacaca tgcacgatca
      541 ccggactctt ctgtcggctc ccgtttgatg gtcgcgtgag ccggtaatgc agggtctggc
      601 cgtcctcgca gacgacggtg ccgagctgcg gtgtcacgtg gtgggtgagg tagggcgcgt
      661 agttcgaaag cccgtccgcg agctcctgcc cgatggtgtt cacctggggc ccctcaagct
      721 tcctgacccg acaggacggc tgccggtcga cggcgctgaa gcggtcgaga aactgctcgc
      781 ccctgaggtc gacggtaacc tcatgccagc ctggctccgc cgtcaacctg acaggctcct
      841 ccggccccga agcagcggac gcgctgaccg agtagagatg ctgctcctgg gggttgccga
      901 accagccggt gaacaggatg cggtgctcat ccgcgtgaag cacgcggttt acccttccct
      961 cgccgcgagt cagcggcagc tcccggctcc cgtcacagag gtagagatgg ctgtggccgt
     1021 cgcgctccga cgtccagaga aagcgctcgc cttcgaggta gcggaagttg tcgtgcaggt
     1081 ttatccaggt ggccgatctt tcttccagcc cgtctctgga gtcccccgat accgggtcga
     1141 agaagcgcaa ccgcagttcc cgttgatccc ggctctgtgt ctgcaccgcc agctcgctcc
     1201 cggcccagtt cactctcgcc aggtagtcgc tcggcgtctg caggtacgaa atttcggtaa
     1261 ccgcgccgga cgcccagtcc attaccagca gcgcgacggc cgcgttggcg ccgcctgtaa
     1321 acggatagcg ctgctccacg gtgcggatct gatcggcatc gatctccaac cggttggtga
     1381 cggggatggg agactcgtcc actcgggtgt aggcgatcct ggttccgtcg ggcgaccacc
     1441 agtagccgtc gaatcggtgc atctcctcct gggcgatgaa ctctgccgct ccgtaggaga
     1501 cggtgccccc gccatcgttg gtgatggcgc gctctgacga agctgcgaga tcccagcaga
     1561 gcagattgcc gtctctgaca taggtgatgt ggcgcccgtc cggcgagaaa cgcaggtccg
     1621 tctggcgagt tccggcaggc gtgaaccgat cgacgcggcc gttggccgta tccatcagat
     1681 agccggcgcc ggcagccgtg accagcacct tgtcaccgcc ggggctgaac gcaaagccgg
     1741 agatcccgcc ctgaaactgg cggcggcgtt cgcgttcggc tttttcggcc gccgtcgggc
     1801 tcgatccctg gtcgtcaatc tgtcttgcat ccagccagca ggccaggtca ccgttgccgc
     1861 aatccaggcg ccacaggtcc tggcgctcgc gatcgtcttc ggcggtgcgc agaaatgccg
     1921 cccggcggcc gtcaggagag aactgacccg cggcgggcac gctgcccgtc aacggcggat
     1981 cgctgaaaag ccgctcgacg gtcagcttca tagattggcg aaggcgtt
//
LOCUS       DBNHQR010000110         2831 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_770615, whole genome shotgun sequence.
ACCESSION   DBNHQR010000110 DBNHQR010000000
VERSION     DBNHQR010000110.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_770615"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..480
                     /locus_tag="AC16VR_02435"
     CDS             1..480
                     /locus_tag="AC16VR_02435"
                     /inference="COORDINATES: protein motif:HMM:NF049802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:226..228,aa:Sec)
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HIR6010040.1"
                     /translation="MTPRSGAVAGSVALLDISKPRGSVLLDRLEELLKERLPGIVVNR
                     YAKPTFTKPAPETLRREIFASNDFVVEGLADUGSCTTCSLHDTVWFEIQGKPAVSIAS
                     SEFGDAADTQARALGMEDACCVFVPHPIQDATDDEMRAKADGVVEAVIEALTVGGSG"
     gene            526..1374
                     /locus_tag="AC16VR_02440"
     CDS             526..1374
                     /locus_tag="AC16VR_02440"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA dioxygenase family protein"
                     /protein_id="HIR6010041.1"
                     /translation="MSEQGREGLQIKPVGGALGAEVTGVDLCQQLPDEHFETIRNAFA
                     EHGVLFFRDQKLAPDDQVRFAERWGPININRFFRPVDGYPMIAEVRKEPDQRVNIGGG
                     WHTDHSYDDVPAMGSLLYAIDVPEQGGDTQFASMYAAYDALSDGLKEMLGRLRAVHSS
                     RHVFGSAHPADPSGRIGNPELAVQDAVHPVIITHPLSGRRALYVNPGFTVRFEDWTEE
                     ESKPLLLYLYQHASRAEFTYRFRWQPGSLALWDNRATWHYAVNDYHGKRRLLHRITIE
                     GEPLGP"
     gene            complement(1428..>2831)
                     /locus_tag="AC16VR_02445"
     CDS             complement(1428..>2831)
                     /locus_tag="AC16VR_02445"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HIR6010042.1"
                     /translation="LDRFGQIEPKVLFACDGYHYNGKPISTVDKARQVAERIPSIRAI
                     VWISLAGEDLVESDGVAYASLLEGDHDLAFAQRAFDDPLYVRYSSGTTGKPKCIVHGI
                     GGTLIQHLKEHQLHTDLRRDDRLFYFTTCGWMMWNWLVSGLATGATLVLYDGSPFHPG
                     PEALLDMAEQEDVTVFGVSARYIAALQKTSLKPMETHDLSPLRAILSTGSPLTHEGFR
                     YVYEHVKRDVLLASISGGTDIISCFVLGNPTLPVWEGEIQCRGLGMAVEVVDDGGRPV
                     TGEKGELVCTRPFPSCPLGFWNDTDGAKFRAAYFQRFPGIWAHGDFAEVTEHDGFVIH
                     GRSDAVLNPGGVRIGTAEIYRQVEHVDAVLESLCVGQEWEGDTRVVLFVVMRPGRELD
                     EALAQQIRQRIRQHASPRHVPARIVQVADIPRTLSGKIVELAVRETIHGRPVQNTSAL
                     ANPEALDLYSNLPELES"
BASE COUNT          509 a          862 c          968 g          492 t
ORIGIN      
        1 ctgacgccga gaagcggcgc ggttgcgggc agcgtggcgc tgctggacat ctccaagccg
       61 cgtggcagcg tgctgctcga tcggttggag gagctgttga aagagcgtct gccgggcatc
      121 gtggtcaacc gctacgcgaa gccgacgttc accaagccgg cgcctgaaac gctgcgccgt
      181 gagatattcg cgagcaacga tttcgtcgtc gaagggcttg ccgactgagg atcctgtacc
      241 acgtgcagtt tgcatgacac ggtatggttc gagattcagg gaaagcccgc ggtttccatt
      301 gcgtccagcg agttcggtga cgcagcggac acccaggcgc gcgcgctcgg gatggaggac
      361 gcctgctgcg tcttcgtgcc gcacccgatc caggacgcga ccgacgacga gatgcgcgca
      421 aaggccgacg gcgtcgtgga ggccgtcatc gaggccctga cggtgggcgg ctcgggttag
      481 ttgagattcg aatcgccccg ggcgaaaggg gggcaggaac gcgcgatgag cgagcaaggg
      541 cgtgaaggat tgcagataaa gcccgttggc ggcgcgcttg gcgccgaggt caccggcgtg
      601 gacctctgcc agcaactgcc cgacgagcac ttcgagacga tccgcaacgc gtttgcggaa
      661 cacggggtgc tgttctttcg cgaccagaag cttgcgcccg atgaccaggt tcgcttcgcc
      721 gaacgctggg ggccgatcaa catcaaccga ttcttccggc cggtggacgg gtacccgatg
      781 atcgccgagg ttcgcaagga gccggatcag cgcgtcaaca taggcggtgg ctggcacacg
      841 gatcactcat acgacgacgt gccggccatg gggtcgctgc tgtatgccat cgacgttccg
      901 gaacagggcg gtgacaccca gtttgccagc atgtacgcgg cctacgacgc gctgtcggac
      961 gggctcaagg agatgcttgg ccggctgcgg gccgttcact ccagccgtca cgtgtttggc
     1021 agcgcgcacc cggcggatcc gagcggtcgc atcggcaatc cggagctcgc ggttcaggat
     1081 gccgtgcacc cggtcatcat cacgcatccc ctgtcaggcc gtcgggccct ttacgtcaat
     1141 ccgggattca ccgttcgctt cgaagactgg accgaggagg agtcgaagcc gctgctgctc
     1201 tacctgtacc agcatgcgtc gcgtgctgag ttcacgtacc ggttccgttg gcagcctggt
     1261 tcgttggccc tctgggataa ccgtgcaacc tggcattacg ccgtcaacga ctaccacggc
     1321 aagcgacgcc tgctgcatcg gatcaccatc gaaggagagc cgctgggccc ttgaggggac
     1381 ggaaccaaga gggggcgcaa agatcgggct ggcgggcgtg ggcggcctca gctttcgagt
     1441 tctggcaggt tgctatagag atctagggcc tccgggttcg cgagagccga cgtgttctgc
     1501 acggggcgcc cgtggatggt ctctcgaacc gccagctcta cgatcttgcc gctcagcgtt
     1561 cgggggatat cggccacctg cacgatgcgc gcgggaacgt gacggggcga tgcatgctga
     1621 cgaatgcgct ggcgtatctg ctgagccagc gcttcgtcca gctcgcggcc gggccgcatc
     1681 acgacgaaga gcacgacgcg ggtgtctccc tcccactcct ggccgacgca caggctttcg
     1741 agcaccgcgt cgacgtgctc tacctgacgg tagatctcgg ccgtgccgat cctgacgcct
     1801 cccggattca gcacggcgtc gctgcgcccg tgaatgacga agccgtcgtg ctcggtgacc
     1861 tcggcgaaat cgccgtgagc ccagatgccg gggaaacgct ggaagtaagc ggcacggaac
     1921 ttcgcgccgt ccgtgtcgtt ccagaagccg agggggcagg agggaaacgg ccgggtgcag
     1981 acgagctcgc ccttttcccc ggtgaccggt cggccgccgt cgtccaccac ctcgaccgcc
     2041 atgccgagcc cccggcattg aatctcgcct tcccaaaccg gcagcgtggg gttgcccaga
     2101 acgaagcagg aaatgatgtc ggtgccgccg gaaatcgaag cgagcagcac gtcccgcttg
     2161 acgtgctcgt agacgtagcg gaagccttcg tgagtcagtg gcgagccggt cgacagaatg
     2221 gctcgcagcg gcgaaagatc gtgagtctcc atgggcttga gcgaagtctt ctgaagggcg
     2281 gcgatgtagc gggcgctgac gccgaacacc gtgacgtctt cctgctcggc catgtccagc
     2341 agggcttcgg gacccggatg aaagggcgag ccgtcgtaga gtaccagcgt ggcaccggtg
     2401 gcgagcccgc tgaccagcca gttccacatc atccagccgc aggtggtgaa gtagaacagc
     2461 cgatcatccc ggcggagatc cgtgtggagc tggtgctcct tgagatgctg gatgagggtg
     2521 ccgccgatcc cgtggacgat gcacttgggc tttccggtgg ttccggacga gtacctgacg
     2581 tacagaggat cgtcaaaagc gcgttgggcg aaagcgagat cgtggtcgcc ttcgaggagc
     2641 gaggcgtagg ctacgccgtc gctttccacc agatcttccc cggccaggga gatccagacg
     2701 atggcgcgta tggaggggat gcgctcggca acctggcgcg ccttgtccac ggtggagata
     2761 ggcttgccgt tgtagtggta accgtcgcag gcgaacagca ccttcggctc gatctgcccg
     2821 aaccgatcca g
//
LOCUS       DBNHQR010000111         4162 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1086742, whole genome shotgun sequence.
ACCESSION   DBNHQR010000111 DBNHQR010000000
VERSION     DBNHQR010000111.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4162)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1086742"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="AC16VR_02450"
     CDS             complement(<1..796)
                     /locus_tag="AC16VR_02450"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6010043.1"
                     /translation="MSRIAQWAIERPLYPWLIMAACFFGGLYGIETVGRLEDPNFPIK
                     TAYIITSYPGASAVEVEQEVTDVIEEALLELPYIDHIISKSVPGRSEVQVDLLEHYGE
                     GDVPQIWDELRRRVVEAAERMPPGAGLPLVEDDFGDVYGLLYAIAAPDYSPADIRDMS
                     RQISTTLKRVPGVAKVQTSGEIGEAIFVELNHERLVRLGLPVDAVFGSIAQENSVVEA
                     GSVSYGQRRIRIAPELAFDSVAAVGDMRVGRPGSVEIIRLGDIADIS"
     gene            complement(800..1861)
                     /locus_tag="AC16VR_02455"
     CDS             complement(800..1861)
                     /locus_tag="AC16VR_02455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6010044.1"
                     /translation="MRRFLILLVLVCAGCEPAAEIVVEEQAVRPARIFRVSSEGVTVE
                     NQFVARVEAAQTVDVSFEVSGPLAELPVREGQTIDKGTLVAALDPTDFQLAVREAEVQ
                     LKLARQDLQRKQKLLADRGISQSVVDDAQALHDLRSVRLAQAREALEDARIVAPFDAY
                     VARRYTDNYANVQAGDKIVRLNDLNEIHVVASVPESLAATGSADQLIGAHAVFPFRPD
                     QRYPLTFRENLGEADAVAQTYEVTFAMPPPPNLQILPGMTATLELEMMAAEGEGRTMN
                     IPTSALIADSDKRFIVWIYDPETQAVSKRTVEVGPAVGRGVAVVSGLANGELIVATGA
                     SQLQNGMKIRVLGDPLTEL"
     gene            complement(1858..2406)
                     /locus_tag="AC16VR_02460"
     CDS             complement(1858..2406)
                     /locus_tag="AC16VR_02460"
                     /inference="COORDINATES: protein motif:HMM:NF037679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6010045.1"
                     /translation="MSAAGLAKTKILSAAAAVVGRSGAAHLTIDAVAAEAKLSKGGVL
                     YHFPSKRAILEGMLDRLISRYSERISRFEAELGDEPDAAIRAVILADRGQDAEERSMA
                     LALLAAAAEDPELLAPARAVIGQWFEAVEQSSGNVQLGILLLLATEGLRFLDMLDLLP
                     AGGTRREQLGQTLLNVAEAGRL"
     gene            2533..3708
                     /locus_tag="AC16VR_02465"
     CDS             2533..3708
                     /locus_tag="AC16VR_02465"
                     /inference="COORDINATES: protein motif:HMM:NF015963.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HIR6010046.1"
                     /translation="MPKWNNWSGRLAAEPDQIAFVRSEDDAAAVVAAAKRKGHAVRTR
                     GACHSHAPLLVTDGTLVDLSGLSGVISANMETRQAWVWAGTPIYALGRALHDSGLALG
                     NQGDIDRQAIAGATATGTHGTGAALASLSASVAGARMVLADGSILDCGPDAERELWEA
                     ARLNLGAMGIITRLQLTLREPYRLREHGWTEPFDSLLPQIPHLAAGNRHFEFFWFPHN
                     DGAIVKTINETDDDPEYPLAEEGSRCAWSYEVLPNSRVQPHTEMEYSVPADRGPECLR
                     AIRELILSRFPDVRWPVEYRLLAEDDVWLSTAYGRPTATVSVHQDVALDETPYFRACE
                     EIFRSFDGRPHWGKVHYLEPDVLASIHPRWQDWWQARDRADPDGLFLNDYLAAWRPR"
     gene            complement(3722..>4162)
                     /locus_tag="AC16VR_02470"
     CDS             complement(3722..>4162)
                     /locus_tag="AC16VR_02470"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6010047.1"
                     /translation="LDLINAVIDTNLRGPYILSCEVARRLIDAKRPGRMVNISSVGGF
                     RYDGNGAALYSITKSAIARMSECLAVEWSRFNINVNCIAPGAFRSEMMDGMMERMGDF
                     SGSFPRKRLCDPAQLDSTLLYLCAPSSECVTGTIIKVDDGQGSR"
BASE COUNT          748 a         1386 c         1337 g          691 t
ORIGIN      
        1 gggaaatgtc tgcgatgtcg ccgagccgga tgatctccac ggagcccggc cggcccaccc
       61 gcatgtcgcc gacggcggcc acggaatcga aggcgagttc cggcgcgatg cggattcggc
      121 gttggccgta ggaaacggat ccggcttcca ccacgctgtt ctcctgggcg atgctgccga
      181 acaccgcatc gaccggaagg ccgagccgga ccaggcgctc gtgattcagc tccacgaaga
      241 tggcctcgcc aatctcgccg ctggtctgca ccttggccac acccgggacg cgcttgagcg
      301 tggtgctgat ctggcgggac atgtcacgga tgtcggccgg gctgtagtcg ggcgctgcga
      361 tggcgtagag caggccatag acgtcaccga aatcgtcttc tacgagcggc agccccgcac
      421 ccggcggcat gcgctcggcc gcctcaacga ctcggcgcct gagctcgtcc cagatctgcg
      481 gcacgtctcc ctcgccgtaa tgctcgagca ggtctacctg cacttccgac cggccgggga
      541 cggacttcga gattatgtgg tcgatgtagg gaagctccag cagggcttcc tcgatgacgt
      601 cggtgacctc ctgctccacc tccactgccg aggccccggg gtaggacgtg atgatgtagg
      661 cggtcttgat cgggaaattc ggatcttcaa gcctcccaac ggtctctatg ccgtagaggc
      721 caccgaagaa gcaggcggcc atgatcaacc aggggtagag cggtcgttcg atggcccatt
      781 gcgcgatgcg gctcatgatt cagagctccg tcagcggatc gcccagcacg cggatcttca
      841 tgccgttctg gagctggctt gcgccggtgg caacgatcag ctcgccgttg gccagcccgg
      901 aaaccaccgc aactcccctg cccacggccg gacccacctc caccgtccgt ttcgacacgg
      961 cctgggtttc gggatcgtag atccagacga tgaagcgctt gtcggaatcc gcgatcagcg
     1021 cagaggtggg aatgttcatc gtccgacctt cgccctctgc tgccatcatc tccagctcga
     1081 gcgtggccgt catgcccggc aggatctgaa gattcggtgg gggcggcatg gcaaacgtca
     1141 cctcgtaggt ctgcgcaacc gcatcggctt cgccgaggtt ttccctgaag gtcagcggat
     1201 agcgctgatc cggacggaag gggaaaacgg cgtgagcgcc aatcagctga tcggccgaac
     1261 cggtcgcagc caggctttcc ggaacgctgg caaccacatg aatctcgttc aggtcattga
     1321 gccggacaat tttgtcccct gcctggacgt tggcgtaatt gtccgtatac cggcgcgcga
     1381 cgtacgcatc gaagggcgcg acgatgcggg cgtcctccaa cgcctcccgt gcctgggcaa
     1441 gccttacgct gcgcagatcg tggagtgcct gagcatcgtc gaccacggac tgagagatac
     1501 cgcggtccgc cagcagcttc tgcttgcgct gcagatcctg gcgcgcgagc ttgagctgga
     1561 cttcggcttc ccgcaccgca agctgaaagt ccgtggggtc cagggcagcg accagggtgc
     1621 ctttgtcgat ggtctgtcct tcccgaacgg ggagctcggc gagcggcccc gacacctcga
     1681 aggacacgtc caccgtctgc gcggcttcca cccgagccac gaactgattc tccacggtga
     1741 cgccttcgct ggaaacccga aaaatccggg ccggccgaac ggcctgttcc tcgacgacga
     1801 tctcggcagc cggttcgcaa ccagcgcaga ccagcaccag caggatcagg aagcgcctca
     1861 cagccgtccg gcctcggcga cgttgagcaa cgtctgcccg agttgctctc ggcgggtgcc
     1921 gcccgcgggc aggaggtcga gcatgtccag gaatcgcaga ccttccgtgg caagcagcag
     1981 gagaatgccc agctggacgt tgccggacga ttgctccacg gcctcgaacc attgcccgat
     2041 gacggcccgc gccggcgcca ggagctccgg gtcctcggcg gcggcagcca gcagcgccag
     2101 cgccatggag cgttcctcgg cgtcctgccc gcggtcggcc aggatgaccg cccggatggc
     2161 cgcatccggc tcgtcgccca gctcggcctc gaaacggctg atccgctccg aatagcggga
     2221 tatgagacgg tccagcatgc cctccagaat cgcgcgcttg ctgggaaaat gatagagcac
     2281 gccgcctttg gagagcttcg cctcggcggc gacggcatcg atggtcagat gggcagcacc
     2341 gctgcggccc accaccgccg cggcggcgct gagtatcttc gttttcgcca gacctgcagc
     2401 actcatggcc gcaaacgtac cgcaaaaaaa ctataccgtc cagacggtat agtttcgttg
     2461 cgtagaatgc ggcgctaccg tatgcgcaga tccagcccca cgacgggcag aggacacgca
     2521 aaggacacgc atatgccgaa gtggaacaac tggtccggcc gtctcgcagc cgagcccgat
     2581 cagatcgcgt tcgttcgcag cgaagacgat gccgccgccg tggttgccgc agcaaagcgg
     2641 aagggtcacg cggtgcgcac gcggggcgcc tgccacagcc acgccccatt gctggtcacg
     2701 gacgggaccc tggtggacct gtcgggtttg agcggcgtga tctcggccaa catggagacg
     2761 cgccaggcgt gggtgtgggc gggcaccccg atctacgccc tcgggcgagc gctgcacgac
     2821 tcgggacttg ccctgggcaa tcagggcgac atcgaccgtc aggccatcgc cggagccacc
     2881 gcaacgggca ctcacggcac gggggccgct ctcgccagcc tgtctgccag cgtggcggga
     2941 gcacgcatgg ttctcgccga cgggagcatc ctagattgcg gacccgatgc cgagcgggag
     3001 ctgtgggagg cggcccgcct gaatctgggc gccatgggca tcatcacccg gttacagctg
     3061 acgctgcgcg agccttaccg gctgcgggag cacgggtgga cagagccgtt cgactcgctg
     3121 ctgccgcaga tcccgcacct tgcagcagga aaccgccact tcgagttttt ctggtttccc
     3181 cataacgatg gggcgatcgt caagaccatc aacgagacgg acgacgaccc cgagtacccg
     3241 ctggcagagg aagggtcccg ctgcgcctgg agctacgagg tgctgccgaa ctcacgcgtg
     3301 cagccgcaca ccgaaatgga gtacagcgtc cccgcagacc ggggccccga gtgcctgcgc
     3361 gccatacggg agctcattct cagcaggttc ccggacgtgc gctggcccgt ggagtaccgc
     3421 ctcctcgccg aggacgacgt gtggctgtcc acggcttacg gccgcccgac tgccaccgtg
     3481 tccgttcacc aggacgtcgc gttggacgag acaccctact tccgcgcctg cgaggaaata
     3541 ttccgcagct tcgacggtcg tccccattgg ggcaaggttc actacctgga acccgacgtg
     3601 ctggcgagca tccacccgcg ctggcaggac tggtggcagg cccgcgaccg agccgatccg
     3661 gacggcctgt tcctgaacga ctacctcgcc gcttggcggc cccgatagcg aagcgatccg
     3721 gtcagcgcga gccctggccg tcgtccacct tgatgatggt gccggtgacg cactccgatg
     3781 acggcgcgca cagataaagc agggtgctgt ccagctgagc cggatcgcac agccgcttgc
     3841 gcgggaagct gccggagaag tcgcccatcc gctccatcat cccgtccatc atctccgacc
     3901 tgaaagcgcc cggcgcgatg cagttgacgt tgatgttgaa tcgagaccac tccacggcca
     3961 ggcactcgct catgcgcgcg atggccgatt tggtaatgga gtaaagggca gccccgttac
     4021 cgtcgtagcg aaacccgccc acggagctga tgttgaccat gcggccggga cgcttggcgt
     4081 cgatgagtcg acgcgccacc tcgcaggaaa gtatgtacgg accccgcagg ttcgtatcga
     4141 tgacggcgtt gatcagatcc ag
//
LOCUS       DBNHQR010000112         4367 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1466977, whole genome shotgun sequence.
ACCESSION   DBNHQR010000112 DBNHQR010000000
VERSION     DBNHQR010000112.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4367)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1466977"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="AC16VR_02475"
     CDS             <1..760
                     /locus_tag="AC16VR_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010048.1"
                     /translation="RYKFYGFGDFVGDSGINIEIEQEAPLYFANAKWRFWNQLYAGIG
                     FVRGNVETRTRVPLPPEWPREITPSFNVDLGALWVPVQFDSRDHEQFPRRGWLIDGDT
                     KIYRDDFGSDFEAETYKLALNHYRAMRTRDALALRAVIKATGGNAPFFLLSSFGSGPD
                     LRGYPAGRYRDKLMYAVQGEYRWHLHDSWILTGFAGVGEVADEIGDFGDDFLPAAGIG
                     ARYVLSKKHRVSISADLAVGKHGAEFYIGIGEAF"
     gene            complement(771..1400)
                     /locus_tag="AC16VR_02480"
     CDS             complement(771..1400)
                     /locus_tag="AC16VR_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010049.1"
                     /translation="MPIVAIATLAILLAASSGCSMIHQDVGKKPPREASRALADRADA
                     TVDDVLEALGPPHAMSAINDGYAFLYHWYAVEERQLGLSSNWPVLRWFRLAIARADID
                     ATALIVHFDDQGGVRAYGLDQRSIDLGGGTGFQPIFVVTPLVDADEYLEDIWPAHRWG
                     MSMLREAPATLNAHAGVDSGMQGIEMRSTSLKVGQRTLEMQKEQSSSSR"
     gene            complement(1400..1789)
                     /locus_tag="AC16VR_02485"
     CDS             complement(1400..1789)
                     /locus_tag="AC16VR_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010050.1"
                     /translation="MAEPEDIMRIPKYLALCALVALGGCASWSQETGVDNRWRGAEVP
                     VWVTGETTAEDVMTALGPPSQIIALKDQVVYYYLKETMAGSAYVLILYNNAKMRAEYD
                     RAIFFFDDGGKLTRYAYSNETLSHRGG"
     gene            2027..2611
                     /locus_tag="AC16VR_02490"
     CDS             2027..2611
                     /locus_tag="AC16VR_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010051.1"
                     /translation="MKVIRLLLGVIVISGANVALGDGHGGDGLVTFSATQKETLTAIV
                     EAIDHETRVVTVRGPRGNAITFTASDEVRNLDQVAAGDLIDAEYVESLDVEVLPGDGS
                     EPGHGVMGAVARTDEGEMPGVAAAEVNVITAKIVAIDLEASTATLEGPDGALEEIEPM
                     NPSNLERVSVGDIVVFTVTQSVALSVREQRADAE"
     gene            complement(2623..3213)
                     /locus_tag="AC16VR_02495"
     CDS             complement(2623..3213)
                     /locus_tag="AC16VR_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HIR6010052.1"
                     /translation="MRMAIGFATLCIVLTLPSEANSAFGKKKSPEERRENITEMREDV
                     LNRLYDERASAKAQVQDSVGYAVFSNTGVNLFLLSTANGAGVAHDNGAGKDIFMNMFS
                     AGGGIGLGVKKFSAVFIFHTRDAFDQFVEEGWNFTGQADAAATTDAEEDEQVGAAGGT
                     VGINDGVTVYQMTEKGLALQATLQGTKYWKNEDLNN"
     gene            3409..3486
                     /locus_tag="AC16VR_02500"
                     /pseudo
     CDS             3409..3486
                     /locus_tag="AC16VR_02500"
                     /inference="COORDINATES: protein motif:HMM:NF024851.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
     gene            complement(3518..>4367)
                     /locus_tag="AC16VR_02505"
     CDS             complement(3518..>4367)
                     /locus_tag="AC16VR_02505"
                     /inference="COORDINATES: protein motif:HMM:NF052063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HIR6010053.1"
                     /translation="VPAGAEGLNPYLAGRRLTLSRAVESQAEPLRVGDAVVVTYAAEL
                     DGLPAIFLPPLLPELDVDGVSGYADVPKLGDGPPARREEKLTLVFDAGGTFTLPGTKL
                     SWWNSESGRIETTSLEDLSFEVTGPLPSASVQTYDERALLGLAIAVTLVLVLGLIAWK
                     AAPALAHTLQRAAEARRRSEPYAFRQLRRSIRAGNRSAIHADLLAWLKKLNVEHDARA
                     FARVYGNERLVAAIDSLSAHLYGRSQPDADPAELRRGLVAARRRCQRSQGGADRAAGR
                     LPALNP"
BASE COUNT          733 a         1383 c         1444 g          807 t
ORIGIN      
        1 ccgttacaag ttctacggct tcggcgactt cgtcggtgac tcgggcataa acatcgagat
       61 cgagcaggaa gcaccgcttt acttcgccaa cgccaagtgg cgtttctgga accagcttta
      121 cgcgggaatc ggcttcgtcc gcggcaacgt cgagacgagg acgcgggttc ctctgcctcc
      181 cgagtggcca cgagagatca ccccgtcgtt caacgtggac ctcggcgcgc tgtgggtgcc
      241 ggtgcagttc gacagccgcg accacgagca gtttccccga cggggatggc tgatcgatgg
      301 ggacaccaag atctaccgcg acgactttgg cagcgatttc gaagcggaga cctacaagct
      361 cgctctgaat cactatcggg ccatgcgcac ccgggacgct ctggccctgc gtgcggtgat
      421 caaggccacc ggtggtaacg cgccgttctt tctgctcagc agcttcggca gcggccccga
      481 tctgcgcggt tacccggccg gacgctaccg tgacaagctc atgtacgccg tgcagggtga
      541 gtaccgctgg caccttcacg acagctggat cctgacgggc tttgccgggg tgggtgaggt
      601 ggctgacgag atcggcgact tcggcgacga cttcctgccg gcggcgggca ttggtgcgcg
      661 ctacgtgctg tcgaagaagc accgggtgag tatttctgcg gacctggccg tcggcaagca
      721 cggcgccgag ttctacatcg gcatcggcga ggcgttctga ccgcggccgt tcagcggctg
      781 gagctgcttt gctctttctg catctccagg gtgcgctgcc ccaccttcag cgacgtcgag
      841 cgcatttcaa tcccctgcat gccggaatcc acgcccgcat gggcgttcag cgtggcgggg
      901 gcttcgcgca gcatggacat gccccagcgg tgggcgggcc agatgtcctc gagatactcg
      961 tccgcgtcca ccaggggcgt caccacgaaa attggctgga agcccgtgcc cccgcccaga
     1021 tcgatggatc gctgatccag gccataggcc cggacgccac cctggtcgtc gaagtgcacg
     1081 atcaacgcgg tggcgtcaat gtcggctcgg gcgatggcca gccggaacca gcgcagcacg
     1141 ggccagttgg acgagagccc gagctggcgt tcttcaaccg cgtaccagtg gtacaggaag
     1201 gcgtaaccgt cgttgatcgc gctcatggcg tgcggtgggc ccagcgcctc cagcacatcg
     1261 tccacggtgg cgtccgcacg gtcggccagc gctcgggacg cctcgcgtgg cggcttcttg
     1321 ccgacgtcct ggtgaatcat cgagcagccg ctcgatgcgg cgagcaggat cgcgagggtc
     1381 gcgatggcga cgatgggcat cagccgcccc ggtgggaaag cgtctcgttg ctgtacgcgt
     1441 aacgggttag cttgcccccg tcgtcgaaga agaagatggc ccgatcgtac tcggccctca
     1501 tcttcgcgtt gttgtagaga atcagcacat aggccgagcc ggccattgtc tccttcaggt
     1561 agtagtagac cacctgatcc ttgagggcga tgatctggga aggcggcccg agtgcggtca
     1621 tgacgtcctc tgccgtggtt tcgccggtga cccagacggg cacctcggct ccgcgccagc
     1681 ggttgtccac accggtctcc tgactccagc tcgcgcagcc gcccagagcc acaagggcgc
     1741 acagcgccag atactttgga attctcatga tgtcctctgg ttccgccata ccggcgcagg
     1801 cagcgagctt gtcgatctta tcctgccggc ccggcgcaat tggtttccag accatagcag
     1861 gaggggatag cggcctccac tcggattcga tagtgaccgg aggggtgacc gtaagcgctg
     1921 gagtcactcg tgctcgcccc tcctcgcttt cgccgcgccg tccgcgttgc gcgtgaatgt
     1981 ccgtcgccga tatcatcagc cgcgctcaac tcaaggggtc actgccatga aggttattcg
     2041 gctgttgctg ggggttatcg tcatctcggg ggctaacgtc gcacttggcg acgggcacgg
     2101 gggcgatggc ctggttacgt tctcggccac ccagaaggaa acgctcacgg cgattgtgga
     2161 ggccatcgac cacgaaactc gggtggtcac tgtcagggga ccccggggca acgccatcac
     2221 cttcaccgcg tcagacgagg tccgcaatct cgatcaggtg gcggcggggg atctcatcga
     2281 cgcggagtac gtggagtctc tggacgtgga ggtgctgccg ggagatggca gcgagccagg
     2341 gcacggcgtg atgggcgccg ttgctcggac cgatgagggc gagatgcccg gcgtggcggc
     2401 agccgaggtc aacgtgatca cggcgaaaat cgttgccatt gacctcgagg ccagcaccgc
     2461 taccctcgaa ggtcccgatg gggcgctaga ggagatcgag ccgatgaacc cgtccaatct
     2521 ggagcgggtc tcggtgggtg acatcgtggt cttcacggtc acccagtccg ttgcgctctc
     2581 ggtacgcgag cagcgggctg acgccgagta ggcgttcaac cgtcagttgt tgaggtcttc
     2641 gttcttccag tacttggtgc cctgaagggt cgcctgtaac gcgaggcctt tctcggtcat
     2701 ctggtacacg gtgacgccgt cgttgatccc gacggtgccg ccggcggcgc cgacctgctc
     2761 gtcttcctcg gcgtcagtgg tcgccgcggc atccgcctgg ccggtgaagt tccagccctc
     2821 ctcaacgaac tgatcgaagg cgtcgcgagt gtgaaaaatg aacacggcgc tgaacttctt
     2881 gacgccgaga ccgatcccgc cgccggcgga gaacatgttc atgaagatgt ctttgccggc
     2941 cccgttgtca tgtgcgacgc ctgcgccgtt ggccgtggag agcaggaaca gattgacgcc
     3001 cgtgttggag aacacggcgt aacccaccga atcctgaacc tgtgccttgg ccgatgctct
     3061 ttcgtcgtag agcctgttca ggacgtcttc gcgcatctcg gtgatgtttt cccgacgctc
     3121 ctcggggctc ttcttcttgc cgaaggcgga gttggcctcc gacggcaggg tcaggacgat
     3181 gcagagcgtt gcaaatccga tggccatgcg catggttggt tcccctctga tacaaaagaa
     3241 tcccgtcacg cagacggggt gccaagtata agcgctcagc cgtaagtgga aacgccaatt
     3301 ctcagcggcc tctgcagcct tcatcgtttt tggtcgtctt cgagaaagcc ggggaggtct
     3361 ggtcgtggat cctgaagaag tgaacctctc cgccgtcggc gtgcagaagt ccccgaggaa
     3421 ctcgaggtcg acgcgcagtc tgccgccaac gcgtgtccgg tgggcgccat cgtcatcgag
     3481 tattgagggc cctcgcttcg gatctcgaac gatcggctca ggggttcagt gccggtagcc
     3541 ggccggcggc gcggtccgcc ccgccctgac ttcgctgaca gcggcggcgc gcggccacca
     3601 gtcctcgccg aagctctgcc ggatcggcat cgggctgaga gcgcccatag agatgggcgc
     3661 tcagcgaatc gatcgcggcc acgagccttt cgttgccgta aacgcgggca aaggcgcgcg
     3721 cgtcgtgttc cacgttcagc ttcttcaacc aggcgagcag atcggcgtga atggccgagc
     3781 ggttgccggc gcggatgctt cggcgcagct ggcgaaaggc gtagggctcc gagcgccgac
     3841 gcgcttcggc ggccctttgc agggtgtggg cgagagccgg tgcggccttc cacgcgatca
     3901 gcccgaggac gaggaccagc gtgaccgcga tcgccaggcc aagcagtgcc cgttcgtcgt
     3961 aggtctgcac gctggccgac ggcagcggtc cggtcacctc gaaactcaga tcctcgaggc
     4021 tggtagtctc gatccgcccc gactcgctgt tccaccagga cagcttcgtg ccgggcaagg
     4081 tgaacgtgcc cccggcgtcg aaaaccaggg tcagcttttc ctcgcgccgc gccggaggcc
     4141 catcgcccag cttcggcacg tccgcatagc cgcttacccc gtcgacgtcg agttcgggca
     4201 gcagaggcgg cagaaagatc gcgggcaggc catccagctc ggccgcgtat gtcaccacta
     4261 ccgcgtcgcc gacccggagc ggctcggcct ggctctcgac ggcccgcgaa agggtgagcc
     4321 gcctgccggc gaggtagggg ttcaacccct cggcgcccgc gggaacg
//
LOCUS       DBNHQR010000113         4917 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1403582, whole genome shotgun sequence.
ACCESSION   DBNHQR010000113 DBNHQR010000000
VERSION     DBNHQR010000113.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4917)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4917)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4917
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1403582"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="AC16VR_02510"
     CDS             complement(<1..632)
                     /locus_tag="AC16VR_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6010054.1"
                     /translation="MSLVDALEYAAQGDTGLNFYDGSGSLSAALPYAQLAQDARILAT
                     RLLGLGVKRGARVGIVAETDPMFPRFFFACQYAGLIPVALPAGLQMGGGEAYVEQLSR
                     MLESCGAAVAVAPESHLSFLKRATRQLSLRMSGSPEDFDALPAVPVKLLPLSADEPAY
                     LQYTSGSTRFPRGVEMTQTAVLANLTEIAEIGTRITADDRIVSWLPYYHD"
     gene            complement(994..1299)
                     /locus_tag="AC16VR_02515"
     CDS             complement(994..1299)
                     /locus_tag="AC16VR_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010055.1"
                     /translation="MCRHHDESASAGRCREERADPPPEKDVANFCDWFQPGKNAFQEG
                     RTTAGENARSRLDALFGDSQGPAEEPDEAPPKPEDGPRSAEEKARAELDRLFSRDAD"
     gene            complement(1503..2273)
                     /locus_tag="AC16VR_02520"
     CDS             complement(1503..2273)
                     /locus_tag="AC16VR_02520"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HIR6010056.1"
                     /translation="MRLVSWNVNGLRACAKKGFLGFLETSGADFVALQEVRAFPDQLD
                     DGLRAPPGWHVCFSPAERPGYSGVALYARAAPDRVQTSLGKPDFDREGRLLSAHFGRL
                     AIVSVYFPKGSGKDRDNSRVGYKLDFYAAVFDHVEQLRRRGPVFVVGDYNTAHEPIDL
                     ARPKTNTKSSGFLPEERAEITRWIDAGWVDVFRRQHPGQPDHYTWWRQWGNARRDNVG
                     WRIDYVLASPSAARRVRQAFIWPEVTGSDHCPVGVDFE"
     gene            2553..3344
                     /locus_tag="AC16VR_02525"
     CDS             2553..3344
                     /locus_tag="AC16VR_02525"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR6010057.1"
                     /translation="MARLISLASGTLPEFGPAEVTRAAAAGGFNGCGIWFDAATWTDE
                     TTRGVAAAFADTGLTPLEIEVIDIRPGAPDPEHQRLLESGAAVGATEAIVVSSDPDEV
                     AVCRRFAHLCELGEQLGVNVCLEFLPIYAVRDLPCALRIIRDVGHPRGKLLIDPLHVA
                     RSGASVADIAAVPGELLSFAQFCDGAKEPPGEPTLDVLREEAVNGRFTPGAGQLPLRD
                     LLDVLPDDLPLSLELRSRPLRERFPDPAERARHVYRETARFLGGA"
     gene            3414..4448
                     /locus_tag="AC16VR_02530"
     CDS             3414..4448
                     /locus_tag="AC16VR_02530"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR6010058.1"
                     /translation="MQVGLVTGKETVDLVEMPDPRPEPGKAVVDVSYCGICGTDVHAY
                     QSGEPYNPAICGHEWMGHVSAAGAGVQVKEGDRVAIGMATACGQCATCVRGDPSHCEM
                     AFAGMVGIGPMAAPHGGFASAIAIEAARLYHVGPEISDEAAAILEPVTIAVHAVRRTG
                     IRLGDSVVVVGAGPIGLLVLQCARAAGAGVCVLVEPQATRRETGAALGADHTIDPRET
                     PDVAAAINSIIGKSGADVVFECAGLARTVEEVPAYVRRGGTVALVGYPTGLSQISAAQ
                     WLAQELKVSTALGYVRDDFEVSKGLVADGRVDCGPLHTSTVSLAGMAGAFAELASEPE
                     QIKILVDPRA"
     gene            complement(4471..>4917)
                     /locus_tag="AC16VR_02535"
     CDS             complement(4471..>4917)
                     /locus_tag="AC16VR_02535"
                     /inference="COORDINATES: protein motif:HMM:NF014615.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HIR6010059.1"
                     /translation="TGLPVWLGVSCRRSTQNASLVAFDFADTPFEAPLDALIAMRPAV
                     VNVMHSEVEATVPAIEAVRERWPGPIGAYPEIGYFAAPDRRFGDDHPPDRLVSEARRW
                     VAAGARLIGGCCGTTPDHIRALHAALPELERLAGETETPGRSPTVR"
BASE COUNT          802 a         1607 c         1654 g          854 t
ORIGIN      
        1 atatcatggt agtagggcag ccatgaaacg atccggtcgt cggcggtgat gcgggtgcct
       61 atctcggcga tttcggtcag gtttgcgagg acggccgtct gggtcatctc gacgccgcgg
      121 gggaagcggg tgcttcccga cgtgtactgc aggtaggcgg gttcgtcggc actcaagggg
      181 agcagcttca ccggcaccgc tggcagggcg tcgaagtcct ctggcgatcc ggacatgcgc
      241 agcgacagct gtcgcgtcgc acgcttcagg aagctgaggt gagattccgg cgcgaccgcc
      301 accgcagctc cgcagctttc cagcatgcga ctgagctgct ccacgtaggc ctcgccgccg
      361 cccatctgca gaccggcagg cagagccacc gggatcaacc cggcgtactg acaggcaaag
      421 aagaatcgcg ggaacatggg gtcggtttcg gcaacgatgc caacccgggc gccgcgtttg
      481 acgcccaggc cgagcagccg ggtcgccagg atccgagcgt cctgcgccag ctgcgcgtac
      541 ggcaacgcgg cgctgaggct cccggagcca tcatagaagt tcagccccgt gtcgccttgc
      601 gcggcgtact ccagggcatc aaccaggctc ataaagccac gtgtgcggtg cgatatcccg
      661 ctacaacgag tggtagggct catgtgataa ccagctcaaa ttgtcgtgtt tgctcttttc
      721 gttgtgctcg aacccctccg aacacagctg cccaacaaac cgaggctctc catcggccgg
      781 cgaatcatcc ggcacttata tttctggtgg cgcagtctac ctgcaaattc gtctgagagc
      841 gatagtgtct tttcaaacga aatcagagac ctacggattg tagacccgcg ataacgggtg
      901 cgtttcacgg aaatggcgtg tgaatgtcac agatttgcga tgtcgctggt cgggcccatc
      961 tcgcctcgga gcgatatccg acgcggggcg caatcagtca gcgtcccgcg aaaacaggcg
     1021 atcgagctcg gcgcgggcct tctcctcggc gcttcggggc ccatcctcgg gttttggcgg
     1081 cgcctcgtca ggctcttccg ccgggccctg cgaatcgccg aacagcgcat cgaggcgtga
     1141 gcgggcattc tcgcccgccg tggtgcggcc ttcctggaag gcgttcttgc ctggctgaaa
     1201 ccagtcacaa aagttggcaa cgtccttttc gggaggggga tctgcacgtt cctcccggca
     1261 ccttcccgcg ctggcggact catcgtggtg ccggcacatg cgacaacagt gcagttcgtg
     1321 gaagcactta gaacaggttg cttgccggct tatgggtctg ggcacgtcgt cgatcgacgt
     1381 accacaattc cagcaacgtg cgtctgccac gggagactgc ctctggcctg gacgatgggt
     1441 ggactataga aatccgatgc gctcgaggca atgcgggtca atgcgggccc ggtggcgtcg
     1501 tctcattcga agtcaacgcc aaccgggcag tgatcggatc cggtcacctc gggccagatg
     1561 aacgcctgcc tgacccgcct tgcagccgat ggcgaggcca gcacgtagtc gattcgccag
     1621 ccgacgttat cccggcgggc gttgccccac tgccgccacc acgtgtagtg gtcgggctgc
     1681 cccggatgct ggcggcggaa gacgtccacc cagccggcgt cgatccagcg cgtgatctcg
     1741 gctcgctcct cgggcagaaa cccgctcgac ttggtgttgg tttttggacg ggcgaggtcg
     1801 ataggctcgt gggccgtgtt gtagtcgcca accacaaata cgggacctct ccggcgcagc
     1861 tgctcaacgt gatcgaacac ggcggcgtaa aaatcgagct tgtagccgac gcggctgttg
     1921 tcccgatcct tgccgctgcc tttggggaag tagacgctca cgatggccag gcggccgaaa
     1981 tgcgcgctga gcagccgccc ctcccgatcg aagtccggct tcccgaggct ggtctgaacc
     2041 ctatcagggg cggcccgggc gtagagggcg acgccgctgt agccggggcg ctccgcggga
     2101 gagaaacaaa cgtgccagcc gggcggggct cgcaggccat cgtccagctg atcggggaac
     2161 gcgcgcactt cctgcagggc gacgaagtcg gccccggagg tctcgagaaa tccgagaaag
     2221 ccctttttcg cacaggcgcg caggccgttg acgttccagc tgacaagtcg catcgatcca
     2281 gtctcgctcc tgtttgaggg cgggcgggcg gggcgcactt taacatccgg cccgcgacgg
     2341 tccacgccgc gtcggcaaat cgccccgggg aagatgtgca gatccgagtt gagcgcccgg
     2401 ggagcctgcg cccggaggaa tcctgccttc ccgtcgagtc gcccccagtg cctgacggcg
     2461 gcccggcctc gggagctatg gggtagcggg cctgctcgcc agaacgctgc aaatgaagca
     2521 cactggccca ggcagcaatc aggacgatca tcgtggcgcg tctcatatct ctagcatcgg
     2581 gaaccctgcc cgagttcggt cctgccgagg tcacccgggc ggctgccgca ggggggttca
     2641 acggctgcgg tatctggttc gacgccgcca cgtggaccga tgagaccacg cgcggcgtgg
     2701 cggccgcctt tgcggacacc ggtctgacgc cgctggaaat cgaggtcatc gacatccggc
     2761 cgggagcacc ggacccggaa catcagcgtt tgctggagag cggcgctgcg gttggggcca
     2821 ccgaggccat cgtggtctcg agcgatcccg acgaggtggc cgtctgccgc cgattcgccc
     2881 atctctgcga gcttggcgag cagctgggcg tcaacgtctg cctcgaattt ctgccgatct
     2941 acgcggttcg ggatctgcct tgcgccctgc ggatcatccg ggacgtggga catccgcgcg
     3001 gcaagctcct cattgatcct ctgcacgtcg ccaggtcggg cgccagcgtt gcggacatcg
     3061 ccgctgtgcc cggcgagctc ctcagctttg ctcagttctg cgatggggcc aaagagcctc
     3121 caggcgagcc taccctggac gttcttcggg aggaggccgt caacggccgg ttcaccccgg
     3181 gggccggtca actgcccctg cgcgatcttc tggacgtcct gcccgacgac ctgccgctca
     3241 gccttgagct gcgctcccga cccctgcgcg agcgtttccc ggatcccgcg gagcgcgccc
     3301 gacacgtata tcgcgaaacg gcccggttcc tcggcggcgc ctgaaccgtc caaggatgcc
     3361 gctgcgcgcc gactggcgta tcctttggcg caattcgaaa cgggggtaag gccatgcagg
     3421 ttgggttagt tacgggtaag gaaacggtcg atctcgtgga gatgcccgat ccccgcccgg
     3481 agccgggcaa ggcggtggtg gacgtttcct attgcggcat ctgcgggacg gacgttcatg
     3541 cctatcagtc gggcgagcct tacaacccgg ccatctgcgg gcacgagtgg atgggccatg
     3601 tctcggcggc aggcgccggc gtgcaggtca aggaaggcga tcgcgtggcc atcggcatgg
     3661 caaccgcctg tggccagtgc gccacctgcg tgcgcggaga cccgtcccac tgcgagatgg
     3721 cctttgccgg catggtcgga atcggcccaa tggcggcccc ccacgggggc ttcgcctcgg
     3781 cgattgccat cgaggcggct cgtctctacc acgtaggccc ggaaataagc gacgaggccg
     3841 ctgccattct cgagccggtc accattgccg ttcatgcggt ccgccgcacc ggtatccggc
     3901 tcggcgacag cgtggtggtg gtgggtgccg gccccatcgg cctgctggtg ctgcagtgtg
     3961 cccgggccgc cggcgcgggt gtctgcgtgc tggtggaacc ccaggctacc cggcgcgaaa
     4021 ccggggcagc gctgggcgcc gaccacacca tcgatccccg ggagaccccc gacgtggcgg
     4081 ctgccatcaa ctcgatcatc ggcaagtcgg gcgccgacgt cgtgttcgag tgtgcggggc
     4141 tggcgcgcac cgtggaggaa gtccccgcct acgttcgccg gggcggcacc gtggcgctgg
     4201 tgggttaccc caccgggttg tcgcagattt ccgcggcgca atggctcgca caggagctca
     4261 aggtatccac ggcgctcggc tacgtgcggg acgacttcga ggtttccaag ggcctcgtcg
     4321 cggacggccg cgtcgactgc ggtcccctgc atacctcgac ggtcagcctc gcaggcatgg
     4381 cgggagcctt tgccgagctc gccagcgagc cggagcagat caagattctg gtcgaccccc
     4441 gcgcctgacc gttctgcgtt ggagagacca tcaccggacg gtgggcgatc ggcccggcgt
     4501 ctcggtctcc ccggcgagcc tttcgagctc aggcagcgca gcgtgcaacg cccggatgtg
     4561 atccggtgtg gttccgcagc agccgccaat caatcgagcg cccgccgcca cccacctgcg
     4621 agcctcggac accagacggt ccggcgggtg atcgtcgccg aagcgccggt cgggggccgc
     4681 gaagtagccg atctccggat aggcgccgat ggggccgggc catctttcgc ggaccgcctc
     4741 gatggcgggc acggtcgcct cgacctcgct gtgcatgacg ttgaccaccg caggacgcat
     4801 ggcgatcagc gcgtccagcg gcgcttcgaa cggcgtgtca gcgaagtcga aagccacgag
     4861 gctcgcgttt tgcgtcgatc tccggcagct gacgcccaac cagacgggaa gcccggt
//
LOCUS       DBNHQR010000114         3343 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_136876, whole genome shotgun sequence.
ACCESSION   DBNHQR010000114 DBNHQR010000000
VERSION     DBNHQR010000114.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_136876"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2325)
                     /gene="smc"
                     /locus_tag="AC16VR_02540"
     CDS             complement(<1..2325)
                     /gene="smc"
                     /locus_tag="AC16VR_02540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HIR6010060.1"
                     /translation="MRLKQIKLAGFKSFVDPTTVILPSNRCAVVGPNGCGKSNIIDAV
                     RWVMGESSAKQLRGENLTDVIFNGSNSRKPTAIASIELIFSNEDGRIGGEYAAYAEIS
                     VRRQVTREAQSTYFLNGNKCRRRDIMDIFLGTGFGPRSYSIIEQGMISQLVEAKPEDL
                     RVYLEEAAGISKYKERRRETENRVRHTRENLDRLNDIREELDRQLQHLKRQSRAAERY
                     RELKEEERKLTAELHTIRLVALEAVLDAHRERIQQLEVELSRRQAEQQAIDTQIEQGR
                     QAHADTSAAVNQVQGTFYQLGADIARIEEAIQFNQQRVKQLELDLETVTRRSEETGRQ
                     LAMDETQIRELREQLAELEPRRQEAERGDGLAAARLAELEQKHRAWQAEWDEFRNRAS
                     QNERDAEVQASRVEHLDQLLQRLRGRHDQLLDDAQEVPVLASDDVASLAEEIAGIESR
                     RSGLEADTDRTIKALGSIREDLLVRERMVEEARGELQSLRHDLASLQAVQRAALGRDE
                     RSAQDWVDARGLGSAERLGEALAVVPGWEHAVESVLGDDLQGIRVDDVSPFGVALDHL
                     PGAGVTLVEGSFHAESSGELPSLSSLIRSGSLKLDSLLYGIFAAESVEVAFAHRGGLR
                     AGQSIITRQGIWVGPDWVRILPVSEQDSGIIQRTQELETLGLRVEEAELGLAELQRQV
                     VEGRGQAEELEHDRAKLQAEVNDLNVRLGDMKAQHGVKRVQIEEAGARRERIRREREE
                     LDAQVSEETRKLLEAREALAIAERTREQQDVTREE"
     gene            complement(2411..3094)
                     /locus_tag="AC16VR_02545"
     CDS             complement(2411..3094)
                     /locus_tag="AC16VR_02545"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR6010061.1"
                     /translation="MATRANNNAEQQGCAPAISLTEQIAEHLAREIITGRLGANARIQ
                     ELRVADDLGVSRGSVREALLILESRHLIDLLPRRGAIVRDLTGEELVSFSELFTDLAA
                     AFFGKLARVPDLDLGLPAQAVEEMAMALGRNDLEGSIAARKAFVDGCLAELNDVYLVS
                     VLRALLPSALRLAHRAARHTGYEMRDTIRFHHALLEALSRGDDNRVGELVRAFGRREG
                     QLAAECRSS"
BASE COUNT          547 a         1144 c         1027 g          625 t
ORIGIN      
        1 ctcctcacgg gtgacgtcct gttgctctct ggtccgctcg gcgatggcca gggcttccct
       61 tgcctccaga agcttgcgcg tctcctcgga aacctgagcg tcgagctcct cgcgctcgcg
      121 acggatccgc tcccgacggg cgccggcctc ctcgatctgc acccttttca cgccgtgctg
      181 ggctttcatg tcgccgagcc gaacgttcag gtcgttgacc tctgcctgca gcttcgctcg
      241 atcgtgttcg agctcctcgg cctgcccgcg cccttccacg acctggcgtt gcagctcggc
      301 aaggccgagc tcggcttcct cgacccgcag cccgagggtt tccagctcct gagtccgctg
      361 gatgatgccc gaatcctgct cgctgaccgg caggatacgg acccagtcgg gtccgaccca
      421 gatcccctgg cgggtaatga tgctctggcc ggcccgcaac ccgccgcggt gcgcaaaagc
      481 cacctcgacg gactccgccg cgaaaatccc ataaagcagg gaatcgagct tgagactccc
      541 ggatctaatg agggaggaaa ggctcggcag ctctccgctg ctctcggcgt ggaaacttcc
      601 ttccaccagg gtcacgccgg cgcccggcag gtgatccaga gcaacgccga acgggctcac
      661 atcgtcaacg cgtatgccct gaagatcgtc gcccagcacc gattcaacgg catgttccca
      721 tccgggcacc accgccagag cttcacccag acgctcggcc gagccgaggc ctcgggcgtc
      781 gacccaatcc tgcgcagaac gctcgtcacg gcccagggcg gcacgctgaa cggcctgcag
      841 gctggcgaga tcgtgtctga gcgactgcag ctcaccacgg gcctcctcga ccatgcgctc
      901 ccgaaccagc agatcttccc ggatggagcc aagggccttg atggtgcgat cggtgtcggc
      961 ctccaggccg ctccgccgcg attcgatacc ggctatctct tcggccaggg acgccacgtc
     1021 gtcgctggca agaacgggca cctcctgggc atcgtcaagc agctggtcgt ggcggcctcg
     1081 aagacgctga aggagctgat cgaggtgctc cacgcgggag gcctgaacct ctgcgtccct
     1141 ttcgttctga gacgctcgat tcctgaactc gtcccattca gcctgccacg cccggtgctt
     1201 ctgctcgagc tcggccaggc gggcggcggc caggccatcc ccgcgctcgg cctcctgccg
     1261 gcgcggctcc agctcggcga gctgctcacg cagctcgcga atctgcgtct cgtccatggc
     1321 cagctgacgg cccgtttcct ccgagcgccg ggtaaccgtc tccaggtcga gctcgagctg
     1381 cttgacccgc tgctggttga actgaatggc ctcctcgatg cgcgcgatgt ccgcaccgag
     1441 ctggtaaaaa gtcccctgaa cctggttgac ggcggcgctg gtatcggcgt gagcctggcg
     1501 accctgctcg atctgcgtgt cgatggcctg ctgctccgcc tgccggcggc tcagctcgac
     1561 ctcgagctgc tggatccgct cccgatgggc gtcgagcacg gcttcgagcg ccaccagacg
     1621 gatggtgtgc agctcggcag tcagcttgcg ctcttcctct ttgagctctc gatatcgctc
     1681 cgccgcgcgg gattgacgct tgagatgctg aagttgcctg tcgagttcct cacggatgtc
     1741 gttgagccgg tccaggtttt cccgggtatg gcgaacccgg ttctcggtct cccggcgacg
     1801 ctccttgtac ttcgagatcc cggctgcttc ctcaaggtag acccgcaggt cttcgggttt
     1861 cgcctcgacg agctggctga tcatcccctg ctcgatgatg gagtagctgc gcggaccgaa
     1921 tcccgtgccg aggaagatgt ccatgatgtc ccggcgccgg catttgttgc cgttgaggaa
     1981 gtaggtggac tgggcttccc gggtgacctg gcgccgaacc gagatctcgg cgtatgccgc
     2041 gtactcgccg ccgatccggc cgtcctcgtt gctgaagatc agctcgatgc tggcgatcgc
     2101 cgtgggcttg cgcgaattgg aaccgttgaa gatgacgtcg gtgaggtttt caccgcgcaa
     2161 ctgcttcgcc gaactttcac ccatgaccca tcgaaccgcg tcgatgatgt tggacttgcc
     2221 gcaaccgtta gggccaacga cggcgcaccg attgctgggg aggatgacgg ttgtcgggtc
     2281 gacgaaagac ttgaagccag cgagcttgat ctgctttagc cgcatggaga ccgagagatc
     2341 accctgtctt aagaccgcgg tagtctaaag caattgccta ccgagcgtaa cacccaaacg
     2401 accctgaaaa tcaggaactt cggcactccg ctgccagctg accctcgcgc cggccgaatg
     2461 cccgaaccaa ctcccccaca cggttgtcgt cgccgcgtga cagcgcttcg aggagagcat
     2521 ggtgaaagcg gatcgtatcg cgcatctcgt agcccgtgtg tcgggccgcc cgatgggcca
     2581 gacgcagggc cgatggcagg agcgcacgca gcacggagac caggtagaca tcgttgagct
     2641 cggcaagaca gccgtcgacg aacgccttcc tcgccgcgat cgatccttcc agatcgttcc
     2701 ggccgagggc catcgccatt tcctcgaccg cctgggccgg caggccgaga tcgaggtcgg
     2761 gaacgcgggc aagctttccg aaaaaggcag ccgccaggtc cgtgaacagc tcgctgaagc
     2821 tgaccagctc ctctcccgtg agatccctga cgatggcgcc ccggcggggc aggagatcga
     2881 tgaggtggcg gctttccagt atcagcagcg cttcccggac cgaccctctg gacactccca
     2941 gatcgtccgc cacccgaagt tcctgtatcc gggcattggc gccaagtcgg cctgtgatga
     3001 tctcgcgggc cagatgctcc gcgatctgtt cggtgaggct gattgccgga gcacagccct
     3061 gttgctccgc attgttgttg gctctggtcg ccatggcgat ccttcgttgt acggaaccgg
     3121 atgagagcta tgaccgcccc gctttcgatt ggttcgccgt cgcccaaacc ggcccgagca
     3181 ccccctgccg gccgaagcgc taggagggat ccggttcccg cagaacggct tcgaccctgc
     3241 gcggggccgt caatcgcgaa agatccagca actgggagcg ccactcccag tcgcccgcct
     3301 gggccgcggg agcgcctgag cggctcagcc gcaccaccag ctc
//
LOCUS       DBNHQR010000115         1405 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1214107, whole genome shotgun sequence.
ACCESSION   DBNHQR010000115 DBNHQR010000000
VERSION     DBNHQR010000115.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1405)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1214107"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1393)
                     /locus_tag="AC16VR_02550"
     CDS             complement(<1..1393)
                     /locus_tag="AC16VR_02550"
                     /inference="COORDINATES: protein motif:HMM:NF017197.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HIR6010062.1"
                     /translation="MADLGIESPGGSLSLSLGGQLRREASGRLFGNVAGTVGVDDIAF
                     GLHPLQIDLGKLSLDVDLAGEIEIQSGDSPPAISGQIGVAASDLLVDHRDWPARLLGL
                     ETMEAPSVRVDGLERASSDTLSVRGLRLLEEEEVVGLSVGSASVEGIELDTSARALRI
                     GEVSLDDLVARLRRGEQGRLMLLGPAAAEEPSAQPEAAPGADDGAPSFELHVGAVSIG
                     GNSALGLEDLSVEPQVTMELVIDRFRLADLGIGPAAGPMVLDVALTQNGAQLSATGDL
                     ALFADARQVKADLEVVHFDLSQLTPYLPNYRVDRGRLSIQTQLGINGDEVAAENAVLL
                     EKLKVGVKRSGDPGLVEEGLGMPLDVALDLLRDGDDRIKFDLPISGSIHDPKFGTGDI
                     VRLAMQNALQRAALSVATNALQPLGTIMFVGKMAGKAARPRFDPVAFEPGARSLSELG
                     TSYLDKLAGLLLER"
BASE COUNT          262 a          504 c          423 g          216 t
ORIGIN      
        1 gccgttccag cagcaggccg gcgagcttgt ccagatagct cgtgcccagc tcgctgagcg
       61 accgcgcgcc gggctcgaaa gccaccgggt cgaaacgggg ccgtgccgcc tttcccgcca
      121 tcttgccaac gaacatgatg gtgcccagcg gctgcagcgc gttcgttgcg acgctgagcg
      181 ccgcgcgctg cagcgcgttc tgcatggcca gccgcacgat gtcgccagtg ccgaatttcg
      241 gatcgtggat gctgccggag atgggcagat cgaacttgat ccgatcgtcc ccgtcccgaa
      301 gcagatccag agcaacgtcc agcggcatgc cgagaccctc ttccacgagc cccggatcgc
      361 ccgaccgttt gacgcccacc ttcagcttct ccagaagcac ggcgttctcg gctgcgacct
      421 cgtcgccgtt gattcccagc tgggtctgga tcgaaaggcg gccccggtcc acccggtagt
      481 ttggcaggta gggggtcaac tggctgagat cgaaatggac gacttccagg tctgccttca
      541 cctgccgggc gtcagcgaac aacgccagat ccccggtagc ggagagctgc gcgccattct
      601 gggtgagcgc gacgtccagg accatgggcc cggcagccgg gccgataccc agatcggcaa
      661 gccgaaatct gtcgatgacg agctccatgg tcacctgcgg ctcgacggat aagtcctcca
      721 aacccagggc gctgtttccg ccgatgctca ctgcccccac atgaagctcg aacgacggcg
      781 cgccgtcatc ggcacccggc gccgcttccg gctgagcaga cggctcctcg gcggcagccg
      841 gcccaagcag catgagccga ccttgctctc cacgccgtaa ccgggccacg aggtcgtcca
      901 gggagacctc cccgattctc aacgcgcgag cgctggtatc gagttcgatg ccctccacgg
      961 aggccgagcc caccgacagc ccgacgacct cttcctcttc caaaagcctg aggccgcgga
     1021 ccgagagggt gtcagacgac gcgcgctcga gcccgtcgac gcgcaccgac ggcgcctcca
     1081 tggtctccaa acccagcaac cgggcaggcc agtccctatg gtctacgagg aggtcgctcg
     1141 cggccacgcc gatctgcccg gagatcgcag ggggcgagtc acccgattga atttcgatct
     1201 ccccggccag atcgacgtcg agactcagct tacccagatc gatctgcagg ggatgcaaac
     1261 cgaaggctat gtcatcaacc cccaccgtcc cggccacgtt gccgaacagc cgcccggaag
     1321 cctctctgcg cagctgcccg cccaaagaaa gcgatagcga tccgcccggc gactcgatac
     1381 ctagatcggc caccagcgga gcaaa
//
LOCUS       DBNHQR010000116         4679 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_390245, whole genome shotgun sequence.
ACCESSION   DBNHQR010000116 DBNHQR010000000
VERSION     DBNHQR010000116.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4679)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_390245"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /locus_tag="AC16VR_02555"
     CDS             <1..1195
                     /locus_tag="AC16VR_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019365382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HIR6010063.1"
                     /translation="GHDTVMPADTRVDLSVIQALARRLEELPDGFVLQRQVQKIMEDR
                     HKMSFGAMPINWGFAETMAYATLLDEGYGVRLTGQDVGVGTFSHRHAALHNQKDGKRY
                     IPLNSLSETVNFDLYDSLLSEEAVLAFEYGYAATAPDKLVIWEAQFGDFANGAQVVID
                     QFISSGEVKWARLCGLTMLLPHGYEGAGPEHSSARLERFLQLCAQHNMQVCLPTTPAQ
                     VFHMLRRQVVWPARKPLVALTPKSLLRHKLAVSTLEDLANGTFHTVLGEVDEIDPDGV
                     ERVVLCSGKVFYELLEARREANASQVAIIRLEQLYPFPEAELERIMAPYGSVRDVVWC
                     QEEPMNQGAWYASQHHMRRVIMKHNENIYLAYAGRPAFAAPASGHAHLHGVRQQRLVE
                     EALFG"
     gene            1216..2418
                     /gene="odhB"
                     /locus_tag="AC16VR_02560"
     CDS             1216..2418
                     /gene="odhB"
                     /locus_tag="AC16VR_02560"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003114264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HIR6010064.1"
                     /translation="MTIQIKAPTFPESIADGTVAAWHRNVGDQVQRDDLLVEIETDKV
                     VLEVVAPAAGRLTSVLKAEGETVLSEELIAELDETAAGEPKQEVEADEPQQAQQPEPA
                     DDEVSASPAARKLATESGLDIQRIEGTGKAGRITREDVAQAASARPEPPAPTPAAVDE
                     ASVDEGERVVRRVPMTRLRASIARRLVEAQQNAAMLTTFNEVDMGPIMTMRRRYQDEF
                     QARHNGTRLGFMSFFVRASVEALKRFPAVNASIDENDILYHGYYDIGVAVSTERGLVV
                     PVIREADALSLAQIEDQVNDFAARAQDGKLSIEDMSGGTFTISNGGVFGSLLSTPILN
                     PPQTAILGMHKIQDRPVAVDGEVVVRPMMYLALSYDHRLIDGQEAVRFLVTIKGLIED
                     PARILLEL"
     gene            2421..3860
                     /gene="lpdA"
                     /locus_tag="AC16VR_02565"
     CDS             2421..3860
                     /gene="lpdA"
                     /locus_tag="AC16VR_02565"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HIR6010065.1"
                     /translation="MNDKYDVIVIGAGPAGYHAAIRAAQLGLSTACIDKSLDADSNPA
                     YGGTCLNWGCIPSKALLDASHKFVEAKEHFAEIGIRTGELSVDVGQMMARKRQVVQGL
                     TGGVAALFKSNGVTDLPGRGRLLASRQVEYTPHDGDAVVLQAEHVILAPGSVPMEIPP
                     APLTKDLIVDSTGALEFEEVPERLGVIGAGVIGLELGSVWSRLGSEVVVLEALDEFLP
                     MTDERIARDALKIFRNQGLDIRLGTRVMGAEVTDGEVTVTYQDQDGDQSLKVDKLIVA
                     VGRRPFTEGLLAPDCGVNLDERGFLYVNDLCATDAPHVYAVGDVVRGPMLAHKGMEEG
                     VMVAERIAGFKPLVNYECVPAVIYTHPEVAWVGKTEQELKASGDEYNVGSFPFTASGR
                     AMAANDVQGQVKVLADAETDRILGVHVLGPQASELIAQAVIAMEFDACAEDLGLTMFA
                     HPTLSEAVHEAALSVAGHAIHTVNRKKQK"
     gene            3873..>4679
                     /gene="sucC"
                     /locus_tag="AC16VR_02570"
     CDS             3873..>4679
                     /gene="sucC"
                     /locus_tag="AC16VR_02570"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HIR6010066.1"
                     /translation="MNLHEYQAKGLFADYGLPVSQGYAVDTADEAVAAAERIGGGRWV
                     VKVQVHAGGRGKAGGVKLADSLDEVRAFADQWIGKNLVTFQTDEHGQPVSKIYVESCT
                     DIDRELYLGAVVDRGTRRVVFMASTEGGVEIEKVAEETPEKILRVAIDPFVGPQPYQG
                     RQLAFRLGLEGAQIREFTNLFMGLSRLFQELDCSLLEINPLVITTAGNIHCLDAKVNI
                     DGNAMFRQPALAAMRDVSQEDEREAEAAEYDLNYVALDGNIGCMVNGAGLA"
BASE COUNT          893 a         1407 c         1563 g          816 t
ORIGIN      
        1 gggccacgac accgtcatgc ccgcggatac ccgcgtcgat ctcagcgtga tacaggcgct
       61 cgccaggcgg ctcgaggagc tccccgatgg tttcgtgctt cagcgccagg tccagaagat
      121 catggaagat cgacacaaga tgagcttcgg ggcgatgccc atcaactggg gatttgccga
      181 gaccatggcg tacgcaacgc tgctggacga gggttacgga gtccgcctga cgggccagga
      241 cgtgggcgtc gggacctttt ctcaccggca tgccgctctc cacaaccaga aggacggcaa
      301 gcgctacatc cccctgaaca gcctctctga gaccgtcaac ttcgacctct acgacagcct
      361 gctttcggaa gaggcggtgc tcgccttcga gtacggttac gccgcgaccg cgccggacaa
      421 gcttgtcatc tgggaggccc agttcggtga ctttgccaat ggtgcccagg tggtcatcga
      481 ccagttcatc tccagcggcg aggtgaaatg ggcccggctg tgcggtttga ccatgctgct
      541 gccccacggc tatgagggcg ccggacccga gcactcgtcg gcgaggctcg agcggtttct
      601 ccagctctgc gcccaacaca acatgcaggt ctgccttccc accacgccgg ctcaggtgtt
      661 tcacatgttg cgtcgtcagg tggtctggcc cgcgcgcaag ccgctggtgg cgctcacgcc
      721 aaagagcctg ctcaggcaca agctcgccgt gtcgacgctc gaggacctgg cgaacgggac
      781 gtttcacacg gtgctgggcg aagtcgacga gatagatccg gacggcgtcg agcgcgttgt
      841 gctctgcagc gggaaggttt tctacgagct gctggaagcc cgtcgggagg cgaacgcgag
      901 tcaggtcgcc atcatcaggc tcgagcagct ctacccgttt cccgaggcgg agctcgagcg
      961 catcatggcg ccgtacggct cggtacggga cgtggtctgg tgtcaggagg aacccatgaa
     1021 ccagggtgcc tggtacgcct cccagcacca tatgcgccgg gtcatcatga agcacaatga
     1081 aaacatctac ctggcctacg ccgggcgccc ggctttcgcc gccccggcca gcgggcacgc
     1141 gcatctgcac ggcgtgcggc agcagcgcct ggtggaggag gccctgttcg gctaacgggc
     1201 tatgaaggac cgccgatgac catccaaatc aaagcgccaa cgtttccgga atccatcgcc
     1261 gacgggaccg tagccgcctg gcaccggaac gtgggcgatc aggtccagcg cgacgatctg
     1321 ctggtggaaa tagagacgga caaggtggtg ctcgaggtgg tggcacccgc ggcaggacgc
     1381 cttaccagcg tcctgaaagc cgagggggaa acggtcctca gcgaagagct cattgcggag
     1441 ctcgacgaaa ccgcggcggg cgagcccaag caagaagtag aggctgacga gccgcagcag
     1501 gctcagcagc ccgagcccgc ggacgacgag gtcagcgcga gcccggcagc caggaagctg
     1561 gccacggaga gcggcctgga catccagcga atcgaaggca cgggcaaggc tggccggatc
     1621 acgcgcgaag acgtggccca ggccgcctcg gcccggcccg agccgccagc accgacgccg
     1681 gcagcagtcg acgaggcttc cgtcgacgag ggtgaacgtg tggtgcgccg tgtgccgatg
     1741 acgcgcctgc gcgcgagcat cgcccgccgg ctggtcgagg cccagcagaa cgctgccatg
     1801 ctgaccacgt tcaacgaggt cgacatgggg cccatcatga ccatgcggcg tcgctatcag
     1861 gacgaatttc aggcgcgtca caacgggacc cgcctcgggt tcatgtcttt cttcgtgcgc
     1921 gccagcgttg aagccctcaa gcgcttcccc gccgtgaacg cttccatcga tgaaaacgat
     1981 atcctgtatc acggctacta cgacatcggc gtagcggtga gcaccgaacg cgggctcgtc
     2041 gtgccggtaa tccgtgaagc cgacgcgctc agccttgcgc agatcgaaga tcaggtgaat
     2101 gatttcgccg cgcgcgcgca ggacgggaag ctttccatag aggacatgtc ggggggtacg
     2161 ttcaccatct ccaatggcgg ggtttttggg tcgctgctgt cgacgccgat tctcaacccg
     2221 ccgcagacgg ccattctcgg catgcacaaa attcaggatc gcccggtggc ggtggacggt
     2281 gaggtcgtgg tcagaccgat gatgtacctc gcgctgtctt acgatcaccg gctcatcgac
     2341 ggtcaagagg ccgtgcgatt cctggttacc atcaagggcc tgattgaaga ccccgctaga
     2401 attctgctgg aactttaaca atgaatgaca agtacgacgt gatcgtcatc ggcgctggcc
     2461 ctgcgggcta ccatgccgcc atccgcgcag cgcagctggg gctcagcacc gcctgcatcg
     2521 acaaatccct ggatgccgac agcaaccccg cgtacggggg cacctgcctg aactggggtt
     2581 gtatcccgtc caaggcattg ctcgatgcct cccacaagtt cgtcgaggcc aaggagcatt
     2641 tcgctgagat cggcattcga accggcgagt tgagcgtgga tgtcggtcag atgatggccc
     2701 gcaagcggca ggtggtacag gggctcaccg gtggcgttgc ggcgctgttc aaaagcaacg
     2761 gcgtcaccga cctgcctgga agggggcgcc tcctggccag ccggcaggtg gagtacacgc
     2821 cccatgatgg cgacgccgtg gtgctgcagg ccgagcacgt gatccttgcg ccgggttccg
     2881 tgcccatgga gatcccgccg gcgccgctga ccaaggatct gatcgtcgac tcgaccggtg
     2941 ctctcgagtt cgaagaggtg cctgagcgac tgggcgtgat cggcgccggg gtgatcggtc
     3001 tggagctcgg cagcgtctgg agtcgcctcg gctccgaggt ggtggttctg gaagcgctgg
     3061 atgagtttct tcccatgacc gatgagcgaa ttgcccggga tgcgctgaag atctttcgca
     3121 atcaggggct cgatatccgc ctggggaccc gggtaatggg ggcggaggtc accgatggtg
     3181 aggtaaccgt gacgtatcag gatcaggatg gggatcagtc cctcaaggtg gacaagctca
     3241 tcgtggcggt gggcaggcgg cccttcaccg aagggctcct cgcgcccgat tgcggggtca
     3301 atctggacga gcgcggcttc ctttacgtca acgatctctg cgctaccgat gccccccacg
     3361 tctacgccgt gggcgacgtg gttcgtggcc cgatgctcgc gcacaagggc atggaggagg
     3421 gcgtcatggt cgccgagcgc atcgccggct tcaaacccct cgtcaactac gagtgcgtac
     3481 cggcggtgat ctacacacac cccgaggttg cctgggtggg caagaccgag caggagctca
     3541 aagccagcgg ggatgagtac aacgtgggca gtttcccgtt cacggccagt ggccgggcaa
     3601 tggcagccaa cgacgtgcag ggccaggtca aggtgcttgc tgacgccgag actgaccgga
     3661 ttctgggcgt gcacgtgctg gggcctcagg cttcggagct cattgcccag gcagtcatcg
     3721 ccatggagtt cgacgcgtgc gcggaggatc tggggcttac gatgttcgct catccgacat
     3781 tgagcgaagc cgtccacgaa gccgcgctga gcgtcgccgg acatgccata catacggtca
     3841 atcgtaagaa acagaaatag aacggaagtc ttatgaatct acatgagtat caggccaagg
     3901 ggctgtttgc cgactatggg ctgccggtgt cgcaggggta tgcggtagac acggcggacg
     3961 aagccgtggc cgccgccgag cggatcggcg gggggcgttg ggtggtcaag gtgcaggtgc
     4021 acgcaggcgg tcgcggcaag gcgggtggcg tcaagctggc agacagcctc gatgaggtcc
     4081 gcgccttcgc cgatcagtgg atcggcaaga acctggtgac tttccagacc gatgagcacg
     4141 gccagcccgt cagcaagatc tacgtggaaa gctgcacgga catcgaccgg gaactctacc
     4201 tgggcgccgt cgtcgaccgg ggcacccggc gggtggtttt catggcgtcc accgagggtg
     4261 gcgtcgagat cgagaaggtg gcagaggaga cgccggagaa gatccttcgc gttgccatcg
     4321 atccctttgt agggccacaa ccctatcagg gccgtcagct ggccttccgg cttggtctag
     4381 agggcgctca gataagggaa tttacaaatc ttttcatggg tttatctagg ctatttcagg
     4441 aactggattg ctcgcttctg gagatcaacc cgctggtgat taccacggcg gggaatattc
     4501 attgccttga tgcgaaggtc aacatcgacg gcaacgcgat gtttcgccag ccagcccttg
     4561 cggccatgcg cgacgtctcc caggaggatg aacgggaagc cgaagctgcc gagtacgatc
     4621 tgaactacgt cgcgctcgac ggcaacatcg gttgcatggt gaacggcgcg ggcctggcg
//
LOCUS       DBNHQR010000117         2885 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1467477, whole genome shotgun sequence.
ACCESSION   DBNHQR010000117 DBNHQR010000000
VERSION     DBNHQR010000117.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1467477"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="AC16VR_02575"
     CDS             <1..308
                     /locus_tag="AC16VR_02575"
                     /inference="COORDINATES: protein motif:HMM:NF038106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PA4642 family protein"
                     /protein_id="HIR6010067.1"
                     /translation="PNMRPDKTKVVDEVWDDDRIEGFLHKAPLGDERSADYSALLYAY
                     RSMRPDDFATFIRLFVDAGRDVNAKSNGGETLLATIRDHRHGAPFRAILEKAGGSAS"
     gene            327..1031
                     /gene="aat"
                     /locus_tag="AC16VR_02580"
     CDS             327..1031
                     /gene="aat"
                     /locus_tag="AC16VR_02580"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011533432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HIR6010068.1"
                     /translation="MSGLSVLSSHRVGFPPPESALAEPNGLLAVGGSLTPEWLLAAYA
                     RGIFPWFDDDASPLLWWSPDPRAVLLPEELRVTRSLAKRIRNAGFDVTMDQAFADVVA
                     ACAAPRGAEAGTWITADMARAYAELHEMGFAHSVETRLDGELVGGLYGVSLGRMFFGE
                     SMFARQRDASKVALYHLTLRARDWNFSLIDCQIMNEHLRSLGAREMPRAEFLRLVHEN
                     RAEPTRRGSWADETAR"
     gene            1028..1738
                     /locus_tag="AC16VR_02585"
     CDS             1028..1738
                     /locus_tag="AC16VR_02585"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003108766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HIR6010069.1"
                     /translation="MKDETADKQFFITPAHPCSYLPKRNAQTLFLDPRETITPQTYQQ
                     LTEQGFRRSGGHLYRPHCKTCQACVPTRLPVAEFVARRSQRRTLARNTDLAVRVEPAA
                     YSPAHYSLYASYIAGRHGDGDMYPPSPDQFRSFLLSQWSDTLFVNSFLDGKLVAGAVT
                     DKLDDGLSAIYTFFDPALEQRSLGVQSILQQIELARELGLSYLYLGYWIRDAEKMRYK
                     TDYRPTELFVNGRWVRIT"
     gene            1820..2035
                     /gene="infA"
                     /locus_tag="AC16VR_02590"
     CDS             1820..2035
                     /gene="infA"
                     /locus_tag="AC16VR_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HIR6010070.1"
                     /translation="MAKEEQIEMEGTVIDTLPNTMFRVELDNGHVVTAHISGKMRKNY
                     IRILTGDKVKVELTPYDLSKGRITFRK"
     gene            complement(2039..>2885)
                     /locus_tag="AC16VR_02595"
     CDS             complement(2039..>2885)
                     /locus_tag="AC16VR_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6010071.1"
                     /translation="GLKSGEKPIGSFLFAGPTGVGKTEVCRQLANIMGVELLRYDMSE
                     YMERHTVSRLIGAPPGYVGFDQGGLLTEAVTKHPHSIVLLDEIEKAHPEVFNLLLQVM
                     DHGTLTDNNGRKADFRNVVLVMTTNAGAQDMSRPSIGFTQQDHTSDAMEAIKKLFTPE
                     FRNRLDGIIQFHSLPKEVIKTVVDKFLIELQAQLDDKKVTLEVDDAAREWLVVEGYDE
                     KMGARPMQRLIQERIKRQLAEDVLFGALSKNGGTVYVTVKDGDLHLRCEAPEGSADKT
                     PAATT"
BASE COUNT          536 a          955 c          864 g          530 t
ORIGIN      
        1 ggccaaacat gcgaccagac aaaacgaaag tcgtcgacga ggtgtgggat gacgaccgca
       61 tcgaagggtt cctgcacaaa gcgccgctgg gcgatgagcg cagcgccgac tactcagcgc
      121 tgctgtacgc ctaccgcagc atgcggcccg acgatttcgc cacgttcatt cgcttgttcg
      181 ttgacgccgg acgggacgtc aacgcgaaaa gcaacggcgg agaaaccctg cttgccacca
      241 tccgcgacca ccgccatggc gcgcctttcc gcgccattct ggaaaaggcc ggcggtagcg
      301 cgagctgaag cgaagcgcct agaacgatgt ccgggctaag cgtgctatca agccaccgtg
      361 tggggtttcc cccaccagag tccgccctcg ccgaacccaa cggcctgctg gccgtgggcg
      421 gcagcctgac gccggaatgg ctgctggcag cttacgcgcg cggcatcttt ccctggttcg
      481 acgacgatgc atcacccctt ctctggtgga gccccgaccc ccgggccgtg ctcttgccgg
      541 aggaactgcg ggtaacgcgc agccttgcca aacgaattcg caatgcaggt ttcgatgtga
      601 ccatggacca ggcgtttgcg gatgtggtcg cagcatgcgc cgcgccccgc ggcgcggagg
      661 ccggcacctg gatcaccgcg gacatggcac gcgcctacgc cgaacttcac gagatgggct
      721 ttgcccactc ggtggagacc cggctggacg gcgagctggt gggggggctc tacggcgttt
      781 ctctggggcg catgtttttc ggagagtcca tgtttgccag gcagcgggac gcttcgaagg
      841 tcgccctgta ccatctgacc ctgcgcgccc gggactggaa tttctctctc atcgactgtc
      901 agatcatgaa cgagcacctg cgctccctag gcgcccggga aatgccccga gccgagttcc
      961 tccggctagt ccatgaaaac cgggccgagc ctacgcgccg cggatcgtgg gcggacgaga
     1021 cggcccgatg aaagacgaga ccgccgacaa gcagttcttc atcacgcccg cccacccctg
     1081 cagctacctg cctaagcgca acgcccagac gctctttctc gatccccggg agacgatcac
     1141 gccgcaaacc taccagcagc tcaccgagca gggttttcgg cgcagtggcg gccatcttta
     1201 ccggcctcac tgcaagacct gccaggcctg cgtgcccacg cgcctccccg tcgccgagtt
     1261 cgttgcgcgc cggagccaga ggcgaaccct ggcacgcaat acggacctgg ccgttcgggt
     1321 ggaacccgcc gcgtacagcc ccgcccatta ctcgctttac gccagctaca tcgcaggccg
     1381 ccatggggat ggcgacatgt acccaccctc ccccgaccag ttccgttctt ttctcttgag
     1441 tcagtggtcg gacaccctgt tcgtcaacag cttcttggat ggcaagctcg ttgccggggc
     1501 ggtcacggac aagctggatg atgggctttc tgccatctac accttcttcg atccggcgct
     1561 agagcagcgc agcctgggcg tgcagagcat cctccagcag atcgagctcg cccgggaact
     1621 cgggctgtcc tatctgtatc tgggctactg gatccgcgac gcggaaaaaa tgcgctacaa
     1681 gaccgactac cgccccaccg agctgttcgt gaacggacgg tgggtgcgta tcacctgagc
     1741 tttgcatgtg cccggaccat gaggcagaat gccgcacgca aaattcgggc cgcccgtaaa
     1801 cggacggcgt caggagtata tggctaaaga agaacagatc gagatggagg gaacggtgat
     1861 cgacaccctc cccaacacca tgttccgagt tgagctggac aacgggcacg tggtcaccgc
     1921 gcacatctcc ggcaagatgc gcaaaaatta cattcgcatc ctcaccggcg acaaggtcaa
     1981 ggtagagctt accccctacg acctcagcaa aggccgcatc accttccgca aataaacttc
     2041 aagtggtcgc cgccggcgtc ttgtcggcgg acccttcggg cgcttcgcag cgcaggtgca
     2101 ggtcgccgtc tttgaccgtg acgtaaacgg tcccgccatt cttcgacagc gcaccgaaca
     2161 gcacgtcttc ggccagctgg cgcttgatgc gctcctgaat caaccgctgc atgggccggg
     2221 cgcccatctt ctcgtcgtag ccctcgacga cgagccactc cctcgcagca tcgtccacct
     2281 ccagggtgac tttcttgtcg tccagctggg cctggagctc gatgaggaac ttgtccacca
     2341 ccgtcttgat gacctctttg ggcagggagt ggaactgaat gatgccgtcg agccgattgc
     2401 ggaattccgg cgtgaacagc ttcttgatgg cctccatcgc gtccgaggtg tggtcctgct
     2461 gagtgaagcc gatggagggc cggctcatgt cctgggcccc cgcgttggtg gtcatgacca
     2521 gcaccacgtt gcggaaatcg gccttccggc cgttgttgtc ggtgagcgtt ccgtggtcca
     2581 tgacctgcag cagcaggttg aagacttccg gatgggcctt ctcgatctcg tccagcagca
     2641 cgatgctgtg gggatgcttg gtaaccgcct cggtaagcag gccgccctgg tcgaagccca
     2701 cgtatcccgg cggcgccccg atgagtctgg agaccgtgtg ccgctccatg tactccgaca
     2761 tgtcgtagcg cagcagctcc actcccatga tgtttgccag ctggcggcag acctccgtct
     2821 tgcccacgcc cgtagggccg gcaaacagga acgagccgat gggtttctca ccggacttca
     2881 agccc
//
LOCUS       DBNHQR010000118         3005 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1720569, whole genome shotgun sequence.
ACCESSION   DBNHQR010000118 DBNHQR010000000
VERSION     DBNHQR010000118.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1720569"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1283)
                     /locus_tag="AC16VR_02600"
     CDS             complement(<1..1283)
                     /locus_tag="AC16VR_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HIR6010072.1"
                     /translation="MPEDSMPAEPSLIQQLKERILLLDGAYGTMLQALNLDEPDYRGS
                     IFEGHPAPLKGNYDALNLARPELVADVHRAYLGAGADIIKTNSFNSTRIAQGDYALAD
                     RAEELNAAAARIARRCADEFASPEWPRFVAGVLGPTNRTASLSPDVNDPGFRNVTFEE
                     LEENYYEAAQALLDNGADLLLLETVFDTLNAKAAIFAIRQLGEKRGATVPLIVSGTIT
                     DASGRTLSGQTCEAFWASVNHAEPLAVGLNCALGADQLRAYVARLGRVADTYLSVHPN
                     AGLPNAFGEYDESPESMAAKLGEFARSGLVNLVGGCCGTTPAHIAAIRESVRECQPRA
                     IPERRNDLMLSGLELLTIDDASLFVNVGERTNVTGSARFARLIREEAYDEALVVARQQ
                     LENGAQVIDINMDEGMLDSQAAMVRFLQLLAAEPD"
     gene            1458..2039
                     /gene="nfuA"
                     /locus_tag="AC16VR_02605"
     CDS             1458..2039
                     /gene="nfuA"
                     /locus_tag="AC16VR_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HIR6010073.1"
                     /translation="MIEISESAQGYLRDLLANQEEEGVGIRVFVAQPGTPQAETCIAY
                     CRPGEQQEGDVAVAYDGFTAWFEERSAPYLVEAMVDYAEDRMGGQLTIKAPNARLPRV
                     GPDAPLEDRINYVLYNEINPGLAAHGGVVTLVEVLEDMTAILQFGGGCQGCAAVDITL
                     KHGVEKSLLEQIPELTGIKDATDHTMTEHAYYR"
     gene            complement(2030..>3005)
                     /locus_tag="AC16VR_02610"
     CDS             complement(2030..>3005)
                     /locus_tag="AC16VR_02610"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003382491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR6010074.1"
                     /translation="VRASRRAVQNILDHSDHRLLVVVGPCSIHDTDAAREYAQRLKQL
                     ADEVSSELFVVMRTYFEKPRTTVGWKGLINDPYLDDSFRVGEGLEIARRLLLDITSLG
                     LPVSTEALDPITPQYLQDLITWSAIGARTTESQTHREMASGLSCPIGFKNGTDGSLDV
                     AINALESAVSPHRFLGISSSGQVSVIRTTGNRHAHIVLRGGSRGPNYDAESVAACEAA
                     LEKIGQVPNIMVDCSHANSNKDHRRQPDVARSVAEQIENGSRSLMGLMIESNLHEGNQ
                     PVNGGSNLRYGVSVTDACIDWPTTESLLRELAERLGPALRARAKVAQR"
BASE COUNT          506 a          950 c         1004 g          545 t
ORIGIN      
        1 atgtcgggct cggcggccag gagctggaga aagcgcacca tggcggcctg gctgtccagc
       61 atcccctcgt ccatgttgat atcgatgacc tgggccccgt tctccaactg ctggcgggct
      121 acgacgagcg cttcgtcgta ggcctcttcg cgtatcagcc gggcaaaacg cgcggagccc
      181 gtcacgttgg tccgctcgcc cacgttgacg aagagtgagg cgtcgtcgat ggtgagcagc
      241 tccaggccgc tgagcatcag atcgttgcgg cgctcgggta tcgctctggg ctggcattcc
      301 cgcaccgact ctcgaatggc ggcaatgtgc gccggcgtcg tgccgcagca cccgcccacc
      361 agatttacca gcccgctgcg ggcaaactcg ccgagcttgg cggccatgga ctcgggcgac
      421 tcatcgtact cgccgaacgc gttgggcagg cccgcgttgg ggtggacgct gaggtaggta
      481 tcggcaacgc gccccaggcg cgccacgtag gcgcgcagct gatcggctcc cagcgcgcag
      541 ttcagcccca cggccagggg ctcggcgtga ttgacggagg cccagaacgc ttcgcacgtc
      601 tggccggaaa gcgttcgccc gctggcgtcg gtaatcgttc cggagacgat gagcggcacc
      661 gtcgcgcccc gtttctcgcc cagctggcgg atcgcgaaaa tcgccgcttt cgcgttcaag
      721 gtgtcgaaca cggtttcgag cagtagcaga tccgcaccgt tgtcgagaag cgcttgcgcg
      781 gcttcgtaat agttctcctc tagctcctcg aaggtgacgt tgcggaaccc gggatcgttc
      841 acgtccggag agaggctcgc cgtgcggttg gtgggcccga gcacgcccgc gacgaagcgc
      901 ggccattcgg gcgaggcaaa ctcatcggca cagcgtcggg cgatccgtgc ggcggcggcg
      961 ttaagctcct ccgcgcgatc ggccagcgcg tagtcgccct gagcgattct cgtggagttg
     1021 aagctgttgg ttttgatgat gtccgccccc gcgcccagat aggcgcgatg gacgtctgcg
     1081 accagctcgg gacgggccag attgagggca tcgtagttgc ccttcagcgg agccggatgg
     1141 ccctcgaaga tcgagccccg atagtctggc tcatcgagat tcaaggcctg gagcatggtc
     1201 ccgtaggcac catcgagcag caggatgcgc tccttgagtt gctgaatcag tgagggctcg
     1261 gcaggcatcg agtcttcggg catgggttga tgggtatcag tctagccgcc gccaacctga
     1321 gcgcaatcga agttatcgga gatgatcatg agtcaagctc atgcgcgatt gcccggcccc
     1381 cggggttcag ctaccatgcc gcttctattc tgagtgtttt actcaggtat agctcaagca
     1441 cccaccggaa cctagccatg atcgagattt cagagtcggc gcaagggtac ctgcgcgatc
     1501 tgctggccaa ccaggaagag gagggcgtgg gcatccgcgt gttcgtggcc cagcccggca
     1561 cccctcaggc ggagacctgc attgcctatt gccggcccgg agagcagcag gaaggggacg
     1621 tggcggtcgc ctacgacgga tttaccgcct ggttcgagga acgcagcgcg ccgtacctgg
     1681 tcgaagccat ggtcgactac gccgaggacc ggatgggtgg gcagctgacc atcaaggcgc
     1741 ccaatgcccg gcttccccgg gtcggtcccg acgcgccgct cgaggatcgc atcaactatg
     1801 tcctgtacaa cgagatcaat cccggtctgg cggcccacgg cggcgtggtg accctcgtcg
     1861 aggttctcga ggacatgacc gcgatcctgc agttcggcgg aggctgtcag ggctgcgcgg
     1921 cggtggacat cacgctcaag cacggcgtcg agaaatctct cctcgagcag attcccgagc
     1981 tgacgggcat caaggacgcc accgaccaca cgatgacgga gcacgcctac taccgttgag
     2041 ccactttggc tctcgcccgc agtgcggggc ccagccgctc ggcaagctcc cgcagcaacg
     2101 actccgttgt cggccaatcg atgcaggcat cggtcaccga tacgccatag cggaggttgc
     2161 tcccgccgtt gacgggctgg ttgccctcgt gaaggttgct ctcgatcatc agccccatca
     2221 gggagcggct cccgttctcg atctgctcgg ctacgcttcg cgccacgtcc ggctggcggc
     2281 ggtggtcctt gttggagttg gcatggctgc agtcgaccat gatgttggga acctggccga
     2341 tcttctccag ggccgcctca caggccgcga cgctctcggc gtcgtagttg ggcccccggc
     2401 tcccgccgcg cagaacgatg tgggcatgac ggttgccggt agtgcggatc accgagacct
     2461 gtcccgatga gctgatcccc agaaaacggt gcgggctcac ggcggattcc agagcgttga
     2521 tcgcgacgtc cagggagccg tctgtgccgt tcttgaaacc gatcgggcag ctcaaccccg
     2581 acgccatctc ccggtgagtc tgcgactcgg tggtgcgggc accgatggcc gaccaggtga
     2641 tcaggtcctg gagatactgc ggggtgatgg ggtcgagcgc ttcggtgctc accggcaggc
     2701 ccaggctggt gatgtccagc agcagccggc gcgcaatctc caggccctcg ccgactctga
     2761 aggagtcgtc caggtacggg tcgttgatca atcccttcca gccgacggtg gtgcgcggct
     2821 tctcgaagta ggtgcgcatc acgacgaaga gttcggagga tacttcgtcg gccagctgct
     2881 tcagacgctg ggcgtactcc cgggcggcgt cggtgtcgtg gatggagcag gggccgacca
     2941 cgaccagcag ccggtgatcc gagtggtcga gaatgttctg caccgcccga cgggatgccc
     3001 gcacg
//
LOCUS       DBNHQR010000119         1299 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_771585, whole genome shotgun sequence.
ACCESSION   DBNHQR010000119 DBNHQR010000000
VERSION     DBNHQR010000119.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_771585"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="AC16VR_02615"
     CDS             complement(<1..507)
                     /locus_tag="AC16VR_02615"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025008.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR6010075.1"
                     /translation="MKTFAELFQGRQRNGCWTFELGERFNGAFGGTNGGVAAAVSLHA
                     ALGERHELMPAAIDCHFLKGCRPGMVTVITRLLSEGRTLTVIATETLDHTGSLCTTAL
                     VTLVNPDRLEQAVRTQPAICVPADHSTEPLPWQHPKGHRIPLIDTFAPEVVASSDDST
                     TTRIHPGFD"
     gene            complement(504..>1299)
                     /locus_tag="AC16VR_02620"
     CDS             complement(504..>1299)
                     /locus_tag="AC16VR_02620"
                     /inference="COORDINATES: protein motif:HMM:NF046782.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HIR6010076.1"
                     /translation="MVSWNDKDVMLYALGVGARPGKDLDFIYEGRGPKVLPTFAVIPG
                     MRAMGSINKVVELPIARMLHGEQAITLHRPLPPRAELTMSSRISEIWDKGKAGVIGVT
                     ADFSDAGGIIFSTHSTLFWIGGGGFNGESGPSTRDLNQPPDRAPDHEVRDVTREEQGA
                     IYRLSGDRVALHIDPEFARKAGYDKPFVHGLCTYGFVGRAVLHELCGGDPTRFVSLEG
                     RFSERVEFEDEIVTRMWVEEPGNAILQAVTQDGKVALSQARARYRT"
BASE COUNT          217 a          422 c          408 g          252 t
ORIGIN      
        1 atcgaagccc ggatggatcc gagtcgtcgt gctgtcgtcg gagctggcaa cgacttcggg
       61 tgcgaaggtg tcgatcaatg gaattcggtg gcctttcggg tgctgccatg gcagtggctc
      121 ggtggagtga tcagcgggta cgcagatcgc cggttgagtc ctgacggcct gctcgagcct
      181 gtcgggattc accagggtca ccagcgccgt ggtgcagagc gatcccgtat ggtcgagcgt
      241 ctcggtagca atcacggtca gcgttcggcc ttcgctcagc agccgggtga tcacggtcac
      301 catgcccggc cggcagcctt tcagaaaatg gcaatcaatg gctgcaggca tcagctcgtg
      361 ccgttcgccg agggcggcgt gcagcgagac tgccgccgca acgccgccgt tggtgccacc
      421 gaaggctccg ttgaaccgct ctccgagctc gaaggtccag catccgtttc tctgacgccc
      481 ctggaacagt tcggcgaacg tcttcacgtc cgataccggg cccgggcctg ggaaagcgca
      541 actttcccgt cctgggttac ggcctgcagg atggcgttgc ccggttcctc cacccacatg
      601 cgagtgacta tctcgtcttc gaactccacc cgctcggaga atcgcccctc gaggctgacg
      661 aacctggtgg ggtctccgcc gcacaactcg tgcagcaccg cgcggccgac gaagccgtag
      721 gtgcacaacc cgtgcacgaa cggcttgtcg taaccggcct tgcgggcaaa ctctggatcg
      781 atgtgcagcg caacccgatc gccgctcagg cgatagatcg cgccctgttc ctctcttgtc
      841 acgtcccgca cttcgtgatc gggagcgcgg tccggcggtt ggttgaggtc gcgagtgctg
      901 ggcccggatt cgccgttgaa cccgccgcca ccgatccaga acagcgtgga gtgtgtcgag
      961 aatatgatgc cgccggcgtc gctgaaatcg gcggtgaccc cgatgacgcc cgccttgcct
     1021 ttgtcccaga tttccgagat ccggctgctc atggtcagct cggcacgcgg cggaagcgga
     1081 cggtgcaacg tgatggcctg ctctccgtgc agcatccgcg cgatgggcag ttccaccacc
     1141 ttgttgatgc tacccatggc ccgcatcccc ggaatgacgg cgaatgtggg cagcaccttg
     1201 gggccccgac cttcgtagat gaaatcgaga tccttgccgg gccttgcgcc gactcccagc
     1261 gcatagagca tcacgtcctt atcgttccag ctcaccatg
//
LOCUS       DBNHQR010000120         3369 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_390671, whole genome shotgun sequence.
ACCESSION   DBNHQR010000120 DBNHQR010000000
VERSION     DBNHQR010000120.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_390671"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="AC16VR_02625"
     CDS             complement(<1..189)
                     /locus_tag="AC16VR_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010077.1"
                     /translation="MTDRANGTFVSADVSADFDMFPPSSGRVAEDLVFCCGQLVFDRA
                     AYELCGVRTTRTAMKLSRE"
     gene            188..2614
                     /locus_tag="AC16VR_02630"
     CDS             188..2614
                     /locus_tag="AC16VR_02630"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6010078.1"
                     /translation="MAKSVSLASAGLVAMPVVSVDAATGSSVIEEVIVTSSRREQSVQ
                     DIPINITAVSDTMIKELRLDGIAEIARYVPGLTVVDRGPRDEQPDILVRGLNTTGLGP
                     GFISDTVAVYFGEIPLNVDFKPVDMERVEVLIGPQGTLYGQGTLGGAIRYIPKRADTS
                     ALSIDARAKAMSNKESDGLSTDIGVTLNIPLVEDVLAARVNFDRLNDKGFIDYPFVVQ
                     ESGVSNPEPDFSDPADVAANLREVEDANGEEATSIRANLRFTPTDGIDVNLWYLNQEN
                     ETEGRQIANRLAIGTGKYESGLRYEEPNDYENELWAIEATADLRFAEATLIYGETQYD
                     ELGQRDQTDLLLNFEYGYEFFPTFSSFTREILEEESDTLEVRLTSQHGGPLSWVVGYF
                     KNEVSSVGTSEEFTPGFDQFAVDNFGGVQLRPDSLEYYSIDDTEEEETAFYGEVSFAI
                     TDALDVTVGYRNYEFKADSTSGFALPLLDTVFFGDDPDAINVDLGDPNQGKDDGDLFK
                     FNVSYDIENFGLLYATFSEGYRNGGVNSVPLCTPEQIASDQQQLCALEDEVLIDPDTI
                     DNYEIGLKGMFLDNTLSLNLAAFFIDWKDLQVAEVTANGNLPITGNGSAAESKGIEMS
                     GRWLINDNWEVMMSYAYTNAELTETAPGLVGPLDALKGTRLPGHAEHQGSFNVVYNRT
                     VWGDVDMTLNYGLVFSTDVFNIPGGDEDTLVDEDGVPGDRGGEAIPGYDVHHLSASFS
                     RDSWTVQAFVDNLWNEYYITGTRTSRRFLQDENLGPGNTINGFTLRSYGQYVGMPRNI
                     GISVSYTFER"
     gene            2682..>3369
                     /locus_tag="AC16VR_02635"
     CDS             2682..>3369
                     /locus_tag="AC16VR_02635"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6010079.1"
                     /translation="MQDSVAELRKEAARALNQRAYERAAVLCRRLLQQDPKCADGWFL
                     MGMVAAANGRITKALELVGNAALLDADNAEYLAQKARLHLLLSQPEAARETADRALAA
                     RPEDPLTLDTLGVVYTRTGNYDQARQLLQRAVAAADDNPQFHFNLASVEQFLGNLEAA
                     AAGYERSIELHPRFFRARWALSELRKSDSDARGIDVLEHLLADSQLTAEDELYLAHAL
                     AAEYEKDARHD"
BASE COUNT          676 a         1072 c         1060 g          561 t
ORIGIN      
        1 ctcacggctc aatttcattg cggttcgggt tgtacgaacg ccgcaaagtt cataggctgc
       61 gcggtcaaaa acaagctgtc cacaacaaaa aacaagatcc tcggccaccc ggccggaact
      121 tgggggaaac atgtcaaagt ctgcactgac atctgcactg acaaaggtgc cgttcgcccg
      181 atcggtcatg gcgaaatcgg tgtctctggc ttcggccggg ctcgtcgcca tgccggttgt
      241 ctcggtcgat gcggccacgg gctcgtccgt catcgaggaa gtgatcgtca cctccagccg
      301 ccgggagcag agcgtccagg acattcccat caacatcacc gcggtgagcg acacgatgat
      361 caaggagctg cgattagacg gcattgccga gatcgccaga tatgttcccg ggctgacggt
      421 ggtcgaccgg gggccgcggg acgaacagcc ggacattctg gtgcgcggcc tgaacaccac
      481 ggggctgggg cccggattca tcagcgacac cgtggccgtc tacttcggcg agatcccgct
      541 gaacgtcgac ttcaagcccg tcgacatgga gcgggtcgag gtcctgatcg gtccccaggg
      601 tacgctgtac ggccagggca ccctcggcgg cgccattcgc tacataccca agcgggcgga
      661 tacctccgcg ctgagcatcg acgcccgcgc caaggcgatg tccaacaagg agagcgacgg
      721 cctgagcacc gacatcggcg tgacgttgaa catcccgctg gtggaggacg tgctggcggc
      781 acgcgtcaac ttcgaccggc tcaacgacaa gggcttcatc gactacccgt tcgtcgttca
      841 ggaatcgggc gtgtcgaacc cggagcccga cttcagcgac ccggcggacg tcgcagccaa
      901 cctgcgtgag gtggaggacg caaacggtga ggaagccacc tccattcgcg ccaacctgcg
      961 attcaccccc accgacggga tcgacgtgaa cctctggtac ctgaaccagg agaacgagac
     1021 ggagggacgg cagatcgcca accgtctggc gatcggtacc ggcaagtacg aatcgggact
     1081 gcgctacgag gagcccaacg actacgagaa cgagctgtgg gccatcgaag cgaccgccga
     1141 cctgcgattc gccgaagcaa ccctcatcta cggggaaacc cagtacgacg agctcgggca
     1201 gcgcgatcag acggacctgc tgcttaattt cgagtacggc tacgagttct tccccacctt
     1261 ctcttccttc acccgggaga ttttggaaga ggaaagcgac accctggaag tgcggctcac
     1321 ctcccagcat ggcggccccc tgtcatgggt ggtgggctac ttcaagaacg aggtttcgag
     1381 tgtcggaacg agcgaagagt tcacccctgg cttcgaccag ttcgccgtgg acaacttcgg
     1441 cggcgtgcag ctgcggccgg acagcctcga gtactactcg attgacgaca cggaagaaga
     1501 agagaccgcc ttttacggtg aggtctcgtt cgcgatcacg gatgcgctgg acgtgaccgt
     1561 cggctaccgc aattacgagt tcaaggccga cagcaccagc ggatttgcgc tgccgctgct
     1621 ggacacggtg ttctttgggg acgatccgga tgccatcaac gtcgacctgg gcgatcccaa
     1681 tcaagggaag gacgacggtg atctcttcaa gttcaacgtc tcctacgata ttgagaactt
     1741 cgggctgctc tacgccacgt tctcggaggg ctaccgcaat ggcggcgtga actccgtacc
     1801 tttgtgcacc ccggaacaaa tcgccagcga tcagcagcag ctgtgcgcgc tggaggacga
     1861 ggttctcatc gatccagaca ccatcgacaa ctacgagatc ggcctcaagg gcatgttcct
     1921 cgacaacacc ctgtcgctga acctggccgc cttcttcatc gactggaagg acctccaggt
     1981 tgccgaggtc accgccaacg gcaacctgcc catcaccggt aacggcagcg ccgctgagag
     2041 caagggcatc gagatgtcgg gccgctggct catcaacgac aactgggaag tgatgatgag
     2101 ctacgcctac accaacgccg agctgacgga gacggcgccc ggcctggtgg gccctctgga
     2161 cgcgttgaag ggcacgcgtc tgcccggcca cgccgagcat cagggatcct tcaacgtcgt
     2221 ctacaaccgg acggtgtggg gcgacgtgga catgacgctg aactacgggc tcgtcttctc
     2281 cacagacgtc ttcaatattc ccggcggcga cgaggacacg ctggtggacg aggacggcgt
     2341 gccgggagac cggggcggcg aggctatccc gggttacgac gtgcatcacc tgtcggcatc
     2401 gttcagccgc gattcctgga ccgttcaggc ctttgtcgac aatctgtgga acgagtacta
     2461 catcacgggc acccgcacct cgcgccggtt cctgcaggac gagaatctcg gacccggcaa
     2521 cacgatcaac ggcttcacgc tgcgctcgta cggccagtac gtgggcatgc cgcgcaacat
     2581 cgggatcagc gtgagctaca ccttcgagcg ttgagcccgc ccttaccggc ctggccagca
     2641 gcagcgttgt atcgttgccg cggccggcct aaggtgcgcg gatgcaagat tcagttgccg
     2701 agctcaggaa agaagctgcc agagcgctga accagcgggc ctacgagcga gccgccgtgc
     2761 tgtgccggcg tcttctgcag caggacccaa agtgcgccga cggatggttc ctgatgggca
     2821 tggtggcggc cgccaacggg cggatcacca aggccctgga gctcgtcggc aacgccgcgc
     2881 tgctggacgc cgacaatgcg gagtacctgg cccagaaggc gaggctgcac ctcctgctga
     2941 gtcagccgga ggcggcccgg gagacggcag accgagcgct cgccgccagg ccggaggacc
     3001 cgctcaccct ggacacgctg ggcgtggtct acacgcgcac gggtaactac gaccaagccc
     3061 ggcagttgct gcagcgcgcg gtggccgccg cagacgacaa tccccagttc cacttcaatc
     3121 ttgcgtccgt ggagcagttt ctcggcaacc tggaagccgc ggccgccggg tacgagcgca
     3181 gcattgagct ccatccgcgc ttcttccggg cgcgctgggc cctgtcggag ctgaggaagt
     3241 cggattcgga tgcccgtggc atcgacgtgc tcgagcacct gctcgccgat tcgcagctca
     3301 cggccgagga cgagctctat ctggcccatg cccttgcggc ggagtacgaa aaggacgcgc
     3361 gccacgacg
//
LOCUS       DBNHQR010000121         6248 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_201335, whole genome shotgun sequence.
ACCESSION   DBNHQR010000121 DBNHQR010000000
VERSION     DBNHQR010000121.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6248)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6248)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6248
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_201335"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2938
                     /gene="gltB"
                     /locus_tag="AC16VR_02640"
     CDS             <1..2938
                     /gene="gltB"
                     /locus_tag="AC16VR_02640"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF008730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0097054 - L-glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HIR6010080.1"
                     /translation="REEVIMALECYIGPERNLLETTEEHAHRLRVPHPILSNEELAAL
                     KHMDHRGWRSRTIDITFKQGTGRDGLVAALDRICAEASGAIADGYSLVVLSDRGIGPD
                     RVPVSSLLAVGAVHHHLVREQSRTRIGIVLETGEAREVHHHCLLVGFGADAINPYLAF
                     EALWQARRNGYLDDAAHITSDDDVVAAYRKGTAKGMLKVMAKMGISTLHSYKGAQIFE
                     AVGLADDVIDVAFVGTASRVQGIGLRVLAEEMERRHVIGYSKRSQNVTVLPNPGDFHW
                     RRTGDTHMWDPSAIASLQVAVRGNSEDAYWQFARHVNEETTRHANLRGLLTFNKDVNG
                     GPIPIEEVEPATEIVKRFATGAMSFGSISAEAHESLAIAMNRLGGKSNTGEGGEDPSR
                     FIPLENGDSKRSAIKQVASGRFGVTINYLTNADELQIKIIQGAKPGEGGELPGHKVDD
                     IIANIRHSTPGVGLISPPPHHDIYSIEDMAQLIHDLKNANPEARISVKLGAEVGVGTV
                     AAGVTKARADHLVIAGDTGGTGASPLTSIKHAGVPWELGIAETHQTLVVNNLRSRVVL
                     QTDGQLKTGRDVAIAALLGAEEFGFATAPLIALGCIMMRKCHLNTCPVGIATQDPVPR
                     KKFAGQPEHVVNYFFMVAKELRMIMAELGFSTMNDMVGRVDALNTDDAIDHWKARGLD
                     LSEILTRAEEPAEFLGSYAIHEQDHGLEKALDNELMQLAEPAIKRGEKVYHELGIVNT
                     NRVVGGMLSNKIIREVGPDMLPDESIHFKFSGSAGQSFGCWLAKGVTLEVEGDANDYV
                     GKGLSGGRLIIYPPKSSPFKPEEQIIVGNVVLYGATSGECYFRGIAAERFCVRNSGAH
                     AVIEGVGDHGCEYMTGGRVVILGETGRNFGAGMSGGIAYVWDPQDRFRTQCNMEMVDL
                     ERLEDSVEIAEVRGMIERHQQYTGSTVAEQVLADWEASLGMFAKVMPVDYKRVLEQRR
                     DEVSAG"
     gene            2941..4404
                     /locus_tag="AC16VR_02645"
     CDS             2941..4404
                     /locus_tag="AC16VR_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007351983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HIR6010081.1"
                     /translation="MGKVTGFMEFDRNAAPYRNAGKRLLDFQEIYTDHDEARLTNQGA
                     RCMDCGVPFCQSNDGCPIHNLIPEWNDLVYRHQWREALDRLHKTNNFPEFTGRVCPAP
                     CEGACVLGITDPAVTIKNIEMAIIDRGFEEGWVKPNPPSQRTGKRIAVVGSGPAGLAA
                     ADQLNKAGHTVIVYERADRIGGLLMYGIPNMKLDKAEVVERRLNLLREEGVEFVTNAH
                     IGDGSQGTISGSDLVNDFDAVLLATGATIPRDLPIPGRDLRGVHFAMEFLTANTKSLL
                     DSGHADGAYISAKDKDVIVIGGGDTGTDCIGTSLRHGCRSLVNFELFPKPPEVRGPDN
                     PWPTWPRIFRTDYGHQEAAERFGSDPRVYAISSTEFFGDEAGNLKGVRTVDVTLKDGR
                     FENVPGSEREWPADLVFLSMGFLGPEHLVSDSLGIAYDDRSNYSAEYGRYATSVDKVF
                     AAGDCRQGQSLVVRAINEGREAAREIDRFLMGASQLP"
     gene            complement(4412..5497)
                     /gene="aroB"
                     /locus_tag="AC16VR_02650"
     CDS             complement(4412..5497)
                     /gene="aroB"
                     /locus_tag="AC16VR_02650"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020302367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HIR6010082.1"
                     /translation="MTLEVDLGDRSYPIHIGDGLLGRGELLRDQVHGSQVAVITNDVV
                     GPLYLPRVAQALAGIDADVYTLPDGEQHKNLESYGAIMDFLMERRHNRSTTLVALGGG
                     VVGDITGFVAATFQRGVAFVQVPTTLLAQVDSSVGGKTAINHPRGKNMIGAFYQPRCV
                     IADTTVLNSLGDRELRAGLAEVLKYGIIADAGFFDWLESNANALLSREPETLAVAIRR
                     SCEIKAAVVAEDERESGIRAILNYGHTFGHAIETLTGYGELLHGEAVAAGMVMAADLS
                     ARQGWLTGTEAKRIKAALEAFGLPVVAPSIEPDDMLSAMSIDKKVVNGRLRLVLARAI
                     GEAAVTEDVDEPALRATLAAGEALCDG"
     gene            complement(5503..6027)
                     /gene="aroK"
                     /locus_tag="AC16VR_02655"
     CDS             complement(5503..6027)
                     /gene="aroK"
                     /locus_tag="AC16VR_02655"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010955625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HIR6010083.1"
                     /translation="MRTQNLFLVGLMAVGKSTVGRCLARDLDMAFYDTDEVLEERAGA
                     EVAWIFDVEGESGFRDREQQVIEELTAKSGVVLATGGGAVLRAANRQNLSARGIVVHL
                     DSPLERLVERTARDNKRPLLQGGDARETLARLQRERAPLYAEIADYTFVTDRQGPKVL
                     ARTIERKLREDGLI"
     gene            complement(6089..>6248)
                     /locus_tag="AC16VR_02660"
     CDS             complement(6089..>6248)
                     /locus_tag="AC16VR_02660"
                     /inference="COORDINATES: protein motif:HMM:NF012485.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HIR6010084.1"
                     /translation="SDTKNVSTTKTPFLGDLPYVGRLFRKELENEEKTELLIFITPKI
                     LQDTVSAR"
BASE COUNT         1186 a         2086 c         1969 g         1007 t
ORIGIN      
        1 ccgcgaggag gtgatcatgg cgctggagtg ctacatcggg ccggaacgca atctgctcga
       61 aaccaccgag gagcacgccc accggctccg ggtgccgcac ccgatacttt ccaacgagga
      121 gctggccgcg ctcaagcaca tggaccaccg cggctggcgt tcgcgcacca tcgacatcac
      181 cttcaagcaa ggcaccggca gagacgggct cgtggccgcg ctcgaccgga tctgcgcgga
      241 agccagtggc gccatcgccg acggctacag cctcgtcgtt ctctcggacc gcggtatcgg
      301 gccggatcgg gtcccggtga gctcgctgct cgccgtcggc gccgtgcacc atcacctggt
      361 ccgtgagcag tccaggaccc gtatcggcat cgtgctggaa accggagaag cccgggaggt
      421 gcaccaccac tgcctgctgg tgggtttcgg cgccgacgcc atcaacccgt atctggcctt
      481 cgaggcgctc tggcaagccc gccgcaacgg ctatctcgac gacgccgccc acatcacctc
      541 agacgacgat gtggtcgccg cctaccgcaa gggcacggcg aaaggcatgc tcaaggtcat
      601 ggcgaagatg gggatctcga ccctgcactc atacaagggc gcgcagatat tcgaggccgt
      661 cggcctcgcc gacgacgtca tcgacgtggc ctttgtcggc accgcaagcc gtgtccaggg
      721 cattggcctg cgggtgctgg ccgaagagat ggagcgccgc cacgttatcg gctattccaa
      781 gcggtcccag aacgtcaccg tgctgcccaa cccgggcgac ttccactggc ggcgcacggg
      841 cgatacgcac atgtgggatc ccagcgccat cgccagcctg caggttgccg tgcgaggcaa
      901 cagcgaggac gcgtactggc agttcgccag gcacgtcaac gaggaaacca cccgtcacgc
      961 caacctgcgc ggcctgctca cgttcaacaa ggacgtcaac ggtggcccta tccccatcga
     1021 ggaggtcgag ccggccacgg agatcgtcaa gcggttcgcc accggcgcca tgagcttcgg
     1081 ctcgatcagc gccgaggccc acgagtcgct ggccatcgcc atgaaccgcc tggggggcaa
     1141 atcgaacacc ggtgagggcg gcgaggaccc cagccgcttc attccgctgg agaacgggga
     1201 ttccaagcgg agcgccatca agcaggtggc cagcggtcgg ttcggcgtga cgatcaacta
     1261 tctcaccaac gccgacgagc tgcagatcaa gatcatccag ggcgccaagc caggcgaggg
     1321 cggcgagctg cccggccaca aggtggacga catcatcgcc aacatccgcc actcgacgcc
     1381 cggcgtcggg ctcatcagcc caccgcctca tcacgacatc tactccatcg aggacatggc
     1441 ccagctgatc cacgacctga agaacgcgaa ccccgaggcg cggatcagcg tcaagctcgg
     1501 cgccgaggtg ggcgtaggaa cggtagcggc cggcgtaacc aaggcccggg ccgatcatct
     1561 ggtcatcgcc ggcgacaccg gcggcaccgg cgcctcgccg ctgacgtcca tcaagcacgc
     1621 gggcgtgcct tgggaactgg ggatcgccga gacccatcag accctcgtgg tcaacaacct
     1681 ccgttcgcgg gtcgttctgc agacagacgg ccagctgaaa acgggccgcg acgtggccat
     1741 cgcggcgctt ctcggcgcag aggagttcgg attcgccacc gcgccgctca tcgcgctggg
     1801 ctgcatcatg atgcgcaagt gtcatctgaa cacctgcccg gtcggcatcg ccacccagga
     1861 tccagttcct cgcaagaagt tcgccgggca accggaacac gtggtgaact acttcttcat
     1921 ggtggccaaa gagctgcgca tgatcatggc cgagctcggc ttctcaacga tgaacgacat
     1981 ggtcggccgg gtggacgcgc tgaacaccga tgacgccatc gatcactgga aggccagggg
     2041 tctggacctc agcgagattc tcacgcgagc cgaggagccg gccgagttcc tgggctccta
     2101 cgcgatccac gagcaggacc acgggctgga aaaggcgctg gacaacgagc tcatgcagct
     2161 cgccgagccg gccatcaagc gcggcgagaa ggtctatcac gagctcggca tcgtcaacac
     2221 caaccgcgtg gtgggcggca tgctgtcgaa caagatcatc cgggaagtgg gtccggacat
     2281 gcttccggac gaatccatcc acttcaagtt ttcgggaagc gccggccaga gcttcggctg
     2341 ctggctggcc aaaggcgtga cgctggaggt agagggagac gccaacgact atgtcggcaa
     2401 aggcctctcc ggcggccgcc tcatcatcta cccgccgaag tcgagcccgt tcaagccgga
     2461 agagcagatc atcgtcggca acgtcgtgct ctacggagcc accagcggcg agtgctactt
     2521 ccgcggcatc gccgcggagc gtttctgcgt gcgtaacagc ggggcgcacg cagtgatcga
     2581 gggcgtcggc gaccacggct gcgagtacat gaccggcggg cgcgtcgtca tcctgggcga
     2641 gacgggacgc aacttcggcg ccggcatgag cggcggcatt gcctacgtct gggatccgca
     2701 agacaggttc cggacccagt gcaacatgga gatggtggat ctggaacgtc tagaggactc
     2761 cgttgagatc gccgaagtgc gcggcatgat cgagcggcac cagcagtaca cgggctcgac
     2821 ggttgccgag caggtgctcg cggactggga ggcttcgctg gggatgttcg ccaaggtgat
     2881 gcccgtcgac tacaaacggg tgctggaaca gcgccgcgac gaagtgagcg cgggttgaac
     2941 atgggtaaag ttaccggatt catggagttc gatcgcaacg cggcgccgta ccggaacgcc
     3001 ggcaaacgtc tgctcgattt ccaggagatc tacaccgatc acgacgaggc gcgcctgacc
     3061 aatcagggcg cgcggtgcat ggactgcggc gttccgttct gccagtccaa cgacggttgc
     3121 cccattcaca atctcattcc cgaatggaac gacctcgtct accgacacca gtggcgggaa
     3181 gcgctggacc ggctgcacaa gaccaacaat ttcccggagt tcaccggccg cgtctgcccg
     3241 gcgccctgcg aaggggcctg cgtgctgggg atcaccgatc cggccgtgac cataaagaac
     3301 atagagatgg cgatcatcga ccgtggtttc gaggaaggat gggtcaagcc caatcctccg
     3361 agccagcgaa cgggcaagcg gatcgccgtg gtcggatccg gccccgctgg ccttgcggca
     3421 gccgaccagc tgaacaaagc cggccacacg gtcatcgtct acgagcgggc ggaccgcatt
     3481 ggcgggctgc tcatgtacgg catccccaac atgaagctgg acaaggccga ggtcgtcgag
     3541 cggcggctga acctcctccg tgaggagggc gtcgagttcg tcaccaacgc ccacattggc
     3601 gacggcagcc aaggcaccat cagcggcagc gacctggtga acgatttcga cgcggtgctg
     3661 cttgccaccg gcgccacgat accgagagat ctgcccattc cgggccggga cctgcgcggc
     3721 gttcacttcg ccatggagtt tctcactgcc aacaccaaaa gcctgctgga ctcgggccac
     3781 gcggacggcg cgtacatctc ggcaaaagac aaggacgtca tcgtgatcgg gggcggcgat
     3841 accggcaccg actgcatcgg cacatcgctg cggcacggat gccggagcct ggtgaatttc
     3901 gagctgtttc caaagccgcc ggaagtccgg ggcccggaca acccctggcc cacctggccg
     3961 agaattttcc ggacagacta cgggcaccag gaggccgccg agcgtttcgg cagcgacccc
     4021 cgcgtctacg cgatctcttc taccgagttc ttcggcgacg aagccggaaa tctgaaaggg
     4081 gtgcggaccg tcgacgtgac gctcaaggac ggcaggtttg agaacgttcc gggatccgaa
     4141 cgggagtggc cggccgacct ggtgtttctc tccatgggtt ttctgggccc cgagcacctg
     4201 gtctccgatt ccctcggaat cgcctacgac gaccgctcca actactcagc cgagtacggc
     4261 aggtacgcca ccagcgtcga caaggtgttc gccgccggcg actgccgaca gggtcagtcc
     4321 ctggtggtgc gagccatcaa cgagggacgc gaggcagcgc gcgagatcga ccgcttcctc
     4381 atgggcgcct cacagctgcc ctgacgggcg gtcagccgtc acacagagct tcgcccgccg
     4441 ccagcgtggc cctcagcgca ggctcgtcga cgtcttcggt gacggccgct tccccgatgg
     4501 cgcgcgcgag caccagtcgc aagcggccat tcacgacctt cttgtcgatg gacatcgcgc
     4561 tcagcatgtc gtccggctcg atggaaggtg ccaccacggg caatccgaac gcctcgagcg
     4621 cagctttgat gcgcttggcc tccgtacccg tcagccagcc ctgccgagcc gaaagatcgg
     4681 ccgccatcac catgcccgcc gccaccgcct caccatgcag gagctcgccg taacccgtca
     4741 gcgtctcgat ggcgtgaccg aaagtgtggc cgtagttcag gatcgcccgg atgccggact
     4801 cccgttcgtc ctcggccacc acggccgctt tgatctcgca ggaccggcgt atcgccacgg
     4861 caagcgtttc cggctccctg ctgagcagcg cgttggcgtt gctctcgagc caatcgaaga
     4921 acccggcgtc cgcaatgatg ccgtacttga gcacctccgc gagcccggca cgaagttccc
     4981 ggtctcccag cgagttcaga accgtggtgt ccgcgatgac gcagcgtggc tggtagaacg
     5041 ccccgatcat gttctttccg cgcggatggt tgatcgccgt cttgccgccc accgacgagt
     5101 ccacctgggc aagcagcgtg gtcggcacct gaacgaaggc caccccgcgc tgaaacgtgg
     5161 cggcaacgaa acccgtgatg tccccgacca ccccgccgcc gagggccacc agcgtggttg
     5221 agcggttgtg ccgccgctcc atgagaaaat ccatgatggc gccgtagctt tccaggttct
     5281 tgtgctgctc gccatcgggc agggtgtaga cgtcggcatc gattcccgcg agggcttggg
     5341 ccacgcgagg caggtacaag ggccccacca catcgttggt gatcacggcg acctggctgc
     5401 cgtgcacctg atcgcgcagc agctctccgc gtcccagcag gccgtcacca atgtgaatgg
     5461 ggtaactgcg atcccccagg tcgacctcga gcgtcacgtc agtcaaatca aaccatcctc
     5521 gcgcagcttg cgctcgatcg tgcgggccag caccttcgga ccctgccggt cggttacgaa
     5581 tgtgtaatcg gctatttccg cgtagagcgg agccctctcg cgctgcaggc gagcgagggt
     5641 ctcgcgggcg tcaccgccct gcagcagcgg cctcttgttg tcgcgcgccg tgcgctccac
     5701 gagccgctcg agggggctgt cgaggtgcac gacgatccct cgagccgaca gattctgccg
     5761 gttcgcagca cgcagcaccg cgccaccacc cgtcgccagc acgacgccgc tttttgccgt
     5821 cagctcctcg atgacctgct gctcccgatc gcgaaagccc gactcgccct ccacgtcgaa
     5881 gatccaggcg acctcggccc ccgcgcgctc ctcgaggacc tcgtcagtgt cgtagaacgc
     5941 catgtcgagg tcccgcgcaa gacaacgccc cacggtactt ttgcccacag ccatcaaacc
     6001 aacgagaaag agattctgcg tgcgcataaa aaagcggcgc ctgaagaggc gccgctagtt
     6061 tagtttattc gggctgggcc cgcgacgatc accgggcgga cacggtgtcc tggagaatct
     6121 tcggcgtgat gaagatcagc aactcggtct tttcctcgtt ctccagctct tttctgaaca
     6181 atctgcccac gtagggaagg tcgcccagga aaggtgtctt ggtagtcgat acattcttcg
     6241 tatccgag
//
LOCUS       DBNHQR010000122         6135 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_834558, whole genome shotgun sequence.
ACCESSION   DBNHQR010000122 DBNHQR010000000
VERSION     DBNHQR010000122.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6135)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6135)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6135
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_834558"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /gene="dprA"
                     /locus_tag="AC16VR_02665"
     CDS             complement(<1..854)
                     /gene="dprA"
                     /locus_tag="AC16VR_02665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HIR6010085.1"
                     /translation="MGDLAGDVESENAAAACLAMELGRPRARAAIAAHGNATSAVEAR
                     AASRDWNPRLVPQRLLADCARHGISLLGGEDACFPELLRHIPDPPLALFSLGALPGRD
                     GTAVAVVGARRCSRLGREVAYQLARQIATAGVRVVSGLALGIDKAAHEGALDAPGAGR
                     TVAVLGCGLARIYPRRNQALAERVVGEGGTLISEYPPEVPPAKHRFPERNRLISGLSH
                     GVVVVEAGDRSGSLITARLALEQGREVMAGPGPVSGGTSRGCHRLLREGAALVECAEH
                     VFEALNLTR"
     gene            complement(963..2036)
                     /locus_tag="AC16VR_02670"
     CDS             complement(963..2036)
                     /locus_tag="AC16VR_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010796361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR6010086.1"
                     /translation="MRKLRIIFQVLIGIGLGISASAWSATDGLAQYLKPGHPEVYTVV
                     KGDTLWDISGKFLDRPWLWPEIWRINPQIDNPHLIYPGDQIALEWVEGQPRLSLRRGD
                     AARTYKMTPADSVSLQPQIRSTPLESAIPTIRLEAIQGFLVQNRVVDPKVLSSAPYVV
                     QGENERLVLGAGDRLYVRGVLPDSESFNVVRKGPLYVDPETKEVLGQEATYIGLGRAV
                     AQEDDIATMSLQSTREEAQIGDRVLPTEERRVDSTFFPSAPDGDVNGQIISVFGGVTQ
                     VGQYDVVVLNRGEREGIEVGNVMAIYKRGALVKDRIANQTIRLPSERAGLLMVFRAFE
                     KLSYGLVLETERPLAVNDEVRNP"
     gene            2333..2836
                     /gene="def"
                     /locus_tag="AC16VR_02675"
     CDS             2333..2836
                     /gene="def"
                     /locus_tag="AC16VR_02675"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010430165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR6010087.1"
                     /translation="MATLDILEYPDPRLRTQAKPVTEVDAQVQQLVEDMFETMYAAPG
                     IGLAASQVNVHQRIIVIDVSENRDEPHVFINPELSLQGAAIESEEGCLSVPGFYEPVI
                     RSEQVIVSALDRDGQSFTLEAQGMLAVCIQHECDHLEGKLFVDYLSNLKRTRIRKKLD
                     KQRRVRA"
     gene            2833..3798
                     /gene="fmt"
                     /locus_tag="AC16VR_02680"
     CDS             2833..3798
                     /gene="fmt"
                     /locus_tag="AC16VR_02680"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003284267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIR6010088.1"
                     /translation="MTRPGGSAPRLGFAGTPEFAAAILQALVTAGHSPVVVYTQPDRR
                     SGRGRKALPSPVKALAAKQGIETRQPATLRETGAAADLAGLELDTLVVAAYGLILPQA
                     ILSAPRYGCMNVHASLLPRWRGAAPVERAIMAGDEETGVTIMQMDAGLDTGPIIEQER
                     CPIESATTGGELEATLARLGARCLLDCLGNLGSLDPTPQDEAGATYAHKLTPADASID
                     WNRPAEAIERQIRALNPRLPATCASGDLRIRILQARAIRDPGGEPGRIVSADRNGIRV
                     SCRESTLVIQSVQLDRGRGRPLSAADAVNGYAAYFEEGTKLAQPS"
     gene            3782..5050
                     /gene="rsmB"
                     /locus_tag="AC16VR_02685"
     CDS             3782..5050
                     /gene="rsmB"
                     /locus_tag="AC16VR_02685"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF008149.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HIR6010089.1"
                     /translation="MPSPAEGAPATASRLLLAVLRDKQTSDQALERIPSTPQVRDTFY
                     GTLRHVFCLQRGVDKALRKPLANKHRDVYCLMLVGAYQLAYARVPDHAAINETVAGCR
                     ALRKPWATGLVNAVLRHIQRKGLPNDCSDASEHPAWMAERIRRDFPDRAGAIFCANMG
                     RAPMCVRINRRLVATDGYRQRLADSGIAFSEGFQDEFIILTAAMPTARLPGFDDGLAA
                     IQDAGAGFAADILAPETGSRVLDACAAPGGKLFHLVERFPDAATLAIDTSTARLERLE
                     AEAARLHHNVNAQQGDATDLRWWDGQPFDRVLLDAPCTGSGTLRRHPDIRLLRAASDL
                     DDYARLQGRLLTNLWQTLRPGGNLLYCTCSLFAEENDEVIGAFLKAADDAEARPLSLA
                     TGVARAHGWQLLPTDSRTDGFYYALLTKRV"
     gene            5064..>6135
                     /gene="trkA"
                     /locus_tag="AC16VR_02690"
     CDS             5064..>6135
                     /gene="trkA"
                     /locus_tag="AC16VR_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016489939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HIR6010090.1"
                     /translation="MKILILGAGQVGGTLAENLAKESIDITLVDNNIEALEELQTKLD
                     IKTVFGSASHPDVLRKAGADDADMLIAVTSSDEVNMVACQVCYSLFRTPQKIARVRSS
                     AYLKGEGFFSGEHMPIDVLINPEEVVTENIRQLLEHPGALQVLGFADDRVQLVAVKAY
                     YGGPLVGQELRFLRQHMPNIDTRVAAIFRRGRAITPEGNTVIEADDEVFFIAAAEHIN
                     AVMSELRRVERPFKRVMIAGGGNIGVKLAQSIEPYFSVKVLEQNARRCEELAEVLDRS
                     VVLNIDATDRELLLEENIEQCDAFCAVTNDDEVNIMSSLLAKRLGVRQVITLIGKPAY
                     VDLMQGGDIDIAISPQQATTSEL"
BASE COUNT         1117 a         2102 c         1918 g          998 t
ORIGIN      
        1 cgggtcaggt tcagcgcttc gaagacatgc tcggcgcact ccaccagcgc ggccccctcc
       61 cgcagcaggc gatggcagcc tcgactcgtc ccgccgctca ccggtccggg accggccatt
      121 acctcccgcc cctgctcgag tgccagtcgc gcggtgatca gagagccgct gcgatcgccg
      181 gcctcgacca cgaccacgcc gtgggagagc ccgctgatca gcctgttgcg ctcgggaaac
      241 cgatgcttcg cggggggcac ttccggcggg tactcgctga tcaatgtgcc gccctcgccc
      301 accacgcgct cggcgagggc ctgattgcgc cgcggataga tccgggcaag cccgcagccc
      361 aagaccgcaa cggtccgacc ggccccaggg gcatcgagcg cgccttcgtg ggcggcttta
      421 tcgataccga gcgccagccc actgaccacc ctgacgcccg ccgtcgcgat ttgccgggcg
      481 agctggtagg cgacctcccg gccgagccgg ctgcaacgcc tggcgcccac cacggcaacg
      541 gcagtcccat ctcgtcccgg cagcgcgccg agagagaaca gtgcaagcgg cgggtcgggg
      601 atgtgccgca acagctcggg aaagcacgca tcctccccgc cgagcagcga gatcccgtgc
      661 cgggcacaat ccgcgagcag ccgttggggt accaggcggg ggttccaatc ccgggacgct
      721 gcccgagctt cgaccgccga ggtcgcattg ccatgagcgg cgattgccgc ccgtgcccgc
      781 ggccgaccaa gctccatggc aaggcaggcc gccgccgcgt tctccgactc cacgtcccca
      841 gccaggtccc ccataaaaaa atcgcccctc gacttgaggg gcgattctat tgaagaccct
      901 gattgccgcc ggtcagcccg gcggccggtc agcggtgcga tttcgccgca ccgtagcgca
      961 aatcagggat tacgtacctc gtcgttgacg gcgagcggac gctcggtctc gagcaccagg
     1021 ccgtaagaga gcttctcgaa cgcccggaag accatcagca gaccggcccg ttcgctgggg
     1081 agccggatgg tctgattggc gatacggtcc ttgacaagag cgccgcgctt gtagatcgcc
     1141 atgacgttgc cgacctcgat gccctcccgc tcgccgcgat tgagcaccac cacgtcgtac
     1201 tggccgacct gggtcacgcc accgaacacg gaaatgatct ggccgttgac gtccccgtcc
     1261 ggcgcgctcg ggaagaacgt ggagtcaacc cgccgctctt cggttggcag cactcggtca
     1321 ccgatctggg cttcctcgcg ggtgctctgc agcgacatgg tggcaatatc gtcttcctgc
     1381 gccacggccc tgcccagccc gatgtaggta gcctcctggc caagcacctc tttcgtctcc
     1441 gggtccacat aaaggggccc tttgcggacg acgttgaagc tttcgctgtc cggcagcacg
     1501 ccccgcacgt agagccggtc gccggcgccc agaaccaggc gctcgttttc gccctgcacg
     1561 acgtatggcg cgctggacag aaccttggga tccaccaccc gattctgcac caggaacccc
     1621 tgaatggcct ccagccgaat ggtggggatg gccgactcca gcggggtgga gcgaatctgc
     1681 ggttgcaggc tcaccgaatc cgccggcgtc atcttgtagg ttcgcgccgc gtcacccctt
     1741 ctcagagaca accggggctg tccctccacc cactcgagcg cgatctgatc gcccgggtag
     1801 atgagatggg gattatcgat ctgggggttt atccgccaga tctccggcca aagccagggc
     1861 cgatccagga acttgccgct gatatcccac agcgtgtcgc ccttgaccac ggtgtaaacc
     1921 tccggatgcc ccggcttgag gtactgagcc aggccgtccg tggcactcca tgcgcttgcc
     1981 gagatcccaa gaccgattcc gatcagaacc tggaaaatga tgcgcagttt cctcatgatt
     2041 cctttccttt tagctgtacc tgctagtcgt gccggaagca tccctgaaga ggatcgccga
     2101 ggctggctcg cttcgaactc ttgccgccgg atgctgtcca tttcgagttg taggccttta
     2161 agtccaaatg aaggctcatt aacatccgct caaccgtcgc cgggtaaggc ttgagcccgc
     2221 gaccgagccg ggtttccgag cgaaaaacat atactcgtca caatgttatc agcctgaatt
     2281 gcagcagaag tagagacaca tcacataggc cgcaaaggcg ctacaacaat ccatggcaac
     2341 cctcgacata ctcgaatatc ccgaccccag gctgcgcact caagccaaac ccgtgactga
     2401 ggtggacgcg caagtacagc aactggtcga agacatgttc gagaccatgt acgccgctcc
     2461 cggaattggc ctggcggcca gccaggtcaa cgtccaccaa cggatcatcg tgatcgacgt
     2521 ttccgagaac cgagacgagc cgcacgtttt catcaatccc gagctttcgc tgcagggggc
     2581 cgccatcgag tcggaggagg gttgcctgtc ggttccgggg ttctacgagc cggtcatccg
     2641 ctccgagcag gtaatagtga gcgcgctcga cagggatgga cagtccttta cgctcgaggc
     2701 ccagggcatg ctggccgtct gcatccagca tgaatgcgac cacctggagg gcaagctctt
     2761 cgtggattac ctgtccaacc tgaagcgcac gcggattcgc aaaaagctgg acaaacaacg
     2821 ccgcgtccgc gcatgaccag gccgggcggc agcgcgccgc gccttgggtt tgcaggcaca
     2881 cccgagttcg cggccgccat cctccaagcg ctcgtcacgg ccggccactc gccggtggtc
     2941 gtgtacaccc agcccgatcg gcgctccggt cgtggccgca aggcgctccc gagccccgtc
     3001 aaggccctgg ccgcgaagca gggcatcgag acccgccagc cggccacgct gagggagacc
     3061 ggggccgcgg cggatctggc ggggcttgag ctcgacacgc tggtggtagc tgcatatgga
     3121 ctgatcctcc cccaggccat cctgagcgcg cccaggtacg ggtgcatgaa cgttcacgcc
     3181 tcgctgctgc cgcgctggcg cggggccgcg ccggtcgagc gagccatcat ggcgggggac
     3241 gaggagacgg gggttaccat catgcagatg gacgccggtc tggacaccgg cccgatcatc
     3301 gagcaggaga ggtgccccat cgaaagcgca accaccggcg gcgagctcga agcaacgctg
     3361 gcgcggcttg gcgcccgctg cctgctggac tgcctgggga atctgggcag cctcgacccc
     3421 acgccccagg atgaggccgg ggcgacgtac gcccacaagc tgacgccggc cgatgccagc
     3481 atcgactgga accgccccgc cgaagcgatc gagcgccaga tccgagctct gaatccaaga
     3541 ttgcccgcca cctgcgcctc cggcgacctg cgcatccgca ttctccaggc gcgggcgatc
     3601 cgggaccctg gcggggaacc cggaaggatc gtctccgctg acaggaacgg tatccgcgtc
     3661 tcctgcaggg agagcaccct ggtcatccag tccgtgcaac tcgatcgcgg gcgcgggcgt
     3721 cccctgtccg cagccgacgc tgtcaacggc tacgccgcct attttgaaga aggcacaaag
     3781 cttgcccagc ccagctgaag gcgctccggc cacggcgtcg cgcctgctgc tcgccgtgct
     3841 cagggacaag cagacgtccg atcaggctct ggagcgcatc ccgtccacac cgcaggtccg
     3901 cgacacgttc tacggcaccc tgcgacacgt tttctgcctg caacgcggtg tggacaaggc
     3961 gctgcgcaag cctctggcga acaagcatcg ggacgtctac tgtttaatgt tagtgggcgc
     4021 ttaccaactg gcatacgccc gcgtaccgga ccacgccgcc atcaacgaga cggtcgccgg
     4081 gtgccgggcg cttcgcaagc cctgggctac gggtctggtc aacgccgttc tccgccacat
     4141 ccagcgaaag ggtctgccga acgattgttc ggatgcttcg gagcatccgg cctggatggc
     4201 tgaacgaatt cgtcgagact ttccggaccg ggcgggcgcc atcttctgcg ccaacatggg
     4261 ccgtgcgccc atgtgcgttc gcatcaacag gagactcgtc gcgacggacg gctaccgaca
     4321 gcggctcgcg gattccggca tcgccttctc ggagggcttt caggacgagt tcatcattct
     4381 gacggcggcc atgccgacgg cccggctgcc aggcttcgac gatgggctgg cggccatcca
     4441 ggacgcgggc gccggattcg ccgcagacat cctggctccg gagacggggt ccagggtgct
     4501 cgacgcctgc gcggccccgg gcgggaagct cttccacctg gtcgagcggt tcccggacgc
     4561 ggccaccctg gccatcgaca cctccacggc acgcctggaa cggctcgaag ccgaggccgc
     4621 tcgcctccat cacaacgtga acgcccagca gggggatgcc acggacctga ggtggtggga
     4681 tggccagccc ttcgatcggg tcctgctgga cgccccctgc acgggcagcg ggaccctgcg
     4741 ccggcacccc gacataaggc tcctgcgggc agcctcggac ctcgacgact acgcccgact
     4801 gcagggacgg ctgctgacta acctgtggca gaccctgcgc cccggcggta accttcttta
     4861 ctgcacgtgc tcgctgtttg cggaagagaa cgatgaggtg atcggggcgt tcctcaaagc
     4921 ggctgacgat gccgaggccc gccctctgtc gctggctacc ggcgtggcca gggctcacgg
     4981 gtggcagctt ctgcccaccg acagccgcac cgacgggttc tactacgctc ttctgaccaa
     5041 gcgggtgtaa caaggcagcc aacatgaaga ttctcattct cggagcgggg caggtgggcg
     5101 gaacgctggc cgagaacctc gccaaggaat ccatcgacat cacgctggtc gacaacaaca
     5161 tcgaagccct cgaggagctg cagaccaagc tggacatcaa gaccgtcttc gggtccgcga
     5221 gccacccgga cgtgctgcgc aaggccggcg ccgacgacgc cgacatgctg attgcggtga
     5281 cctccagcga cgaggtcaac atggtcgcct gccaggtctg ctactccctg tttcgaacgc
     5341 cgcagaaaat cgcgcgggtt cgctcgtcag catatctgaa gggggaaggg ttcttcagcg
     5401 gggagcacat gcccatcgac gtgctgatca acccagagga ggtcgtcaca gaaaacatcc
     5461 gacagctgct cgagcacccc ggagcgcttc aggttctcgg gttcgccgac gacagagtcc
     5521 agctggtggc cgtgaaggcg tactacggcg gccccctggt cggtcaggag cttcgctttc
     5581 tccgccagca catgccgaac atagacaccc gcgtggcggc catcttccgt cgcggtcgcg
     5641 ccatcacgcc cgagggcaac acggtgatcg aggcagacga cgaggtcttc ttcatcgctg
     5701 ccgccgagca catcaacgcc gtgatgagcg agctcaggcg ggtggagcgg cccttcaagc
     5761 gcgtgatgat cgccggcggg ggcaacatcg gcgtcaagct tgcccagagc atcgagcctt
     5821 acttctcggt gaaggtgttg gagcagaacg cccgccgctg cgaggagctg gccgaggttc
     5881 tcgacaggtc cgtggtcctc aacatcgacg ccaccgaccg ggagcttctg ctggaggaga
     5941 acatcgagca gtgcgacgcc ttctgcgccg tcaccaacga cgacgaggtc aacatcatgt
     6001 cctcgttgct ggccaaacgg ctcggggtcc gacaggtgat cacgctcatc ggcaagcccg
     6061 cctacgtcga cctgatgcag ggcggcgaca tcgacatcgc catctcgcct cagcaggcca
     6121 ccaccagcga gctcc
//
LOCUS       DBNHQR010000123         5221 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1214644, whole genome shotgun sequence.
ACCESSION   DBNHQR010000123 DBNHQR010000000
VERSION     DBNHQR010000123.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5221)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5221)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5221
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1214644"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="AC16VR_02695"
     CDS             complement(<1..196)
                     /locus_tag="AC16VR_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010091.1"
                     /translation="MLSNDSISRGTQRATTNTMVDVLDKTRQDLDPKLKEIVEKDERN
                     FVYCGICSHVLTREVERIEVS"
     gene            198..845
                     /locus_tag="AC16VR_02700"
     CDS             198..845
                     /locus_tag="AC16VR_02700"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HIR6010092.1"
                     /translation="MHRAVERKGGRAALDALLPAARSGSDLAALPDRDYLAEMTRCVF
                     RSGFVWKIIENKWPGFEVAFRGFDVAGCAMLSDEDIEQLSADERIVRHAAKIRSVPAN
                     AAFILDVRREAGGFGRYLADWPEDEIVGLWDELKRRGSRLGGQTGRYFLRFVGKDTPI
                     LSRDVVKALIGQGVIDREPTSKKALARVQDAFNEWHAESGRELCQISRVLSLSVD"
     gene            complement(1050..2255)
                     /locus_tag="AC16VR_02705"
     CDS             complement(1050..2255)
                     /locus_tag="AC16VR_02705"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6010093.1"
                     /translation="MTGMVIVGAGHAAGQAAASLRQEGYEGEITIIGDEPHIPYQRPP
                     LSKQYLAGEQGLERVYLRPEKFYADKEITLRMGVSVTAIDTQAHTVTTDDGETIGYEK
                     LLLATGSRPRILNVPGSDLAGIHYLRTIADVDGIRAELAEGRRLVIVGGGYIGLEVAS
                     VGIQAGLEVHVLEMEERILQRVTTPEMSDYYHQLHTGRGVHIHTSTMVSGFTGEGRVG
                     AVQCGDEEIAADIVVVGIGIIPNVEVAQAAGIECDNGIVVDEGCRTSADDVFAAGDCT
                     NHPNPLLHRRLRLESVPNAMEQARVAAANMCGADKVYESIPWFWSDQYELKLQMVGFS
                     ADGDTQVVRGDKAANQFAVFYLKDGAVVAADAVNSPKEFMVCKQLVGKPVDAARLGDP
                     DVELKSLLA"
     gene            2427..3497
                     /locus_tag="AC16VR_02710"
     CDS             2427..3497
                     /locus_tag="AC16VR_02710"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HIR6010094.1"
                     /translation="MRYRTLHEIVRQAQRNLDRDCRDYLLGGADTESSLRRNRHGLDS
                     WVFRPRILNDVSEVNLTIELLGAPMRIPVVLPPIGSVQLFEPGGGQTVAEAAHEFGIL
                     QILSSVCTPDFEAVAAAVPGPRIYQLYLMGDRVWLDDMIGRTIEAGYTGFCLTADTQI
                     YSRRERDILKGFVPPSGSQAGSRDFNYQARMTWETVAHIKSTFDIPLIVKGVNAGEDA
                     ARCVEAGVDVVYVSNHGGRQLDHTRACIDALPEVVEAVAGRVPVIVDGGFLRGADVVK
                     GLCLGADVVGMGRLEGLAMAAGGRGALVRALEIVEQEIRTTMALLGVSDLADLNPGLM
                     ERAQPLAESTVFSAFPLLEEGY"
     gene            3598..>5221
                     /locus_tag="AC16VR_02715"
     CDS             3598..>5221
                     /locus_tag="AC16VR_02715"
                     /inference="COORDINATES: protein motif:HMM:NF024144.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin-like beta sandwich domain-containing
                     protein"
                     /protein_id="HIR6010095.1"
                     /translation="MAPTLRTSIRWFGTFLIVFTMAACGGGGGGDDAPRPPGTDASLS
                     GMSLSSGSLEPAFAAATTEYTADVPFAVAEITLTATTSDSRASLTVDGATASSGSPSD
                     PVALAVGETKVDVVVTAEDGTTTRAYSITLTRAAAATNADLVSLSLSAGALDQIFDTT
                     QPDYTATLGYLAASVLVLAEPEDPNAVVTVNAGTVDERVALDEGVNVIDLLVTAEDGT
                     TTRAFELTITRGAALDFAQRLYVKPDVIRESYRFGAAVDLEGSWLAVGAPGDQSLANT
                     IDGDDGDFAGVNVGGVTVFQADASGLWTRQAYLKAANAGDGDGFGEALAMSGNTLAVG
                     APLEQGLDGAPDDDSGNGVGAVYVFVRDESDVWTQQAYLKASNADNGDEFGRYLDLDG
                     DTLVVGAWHEQSAGAEDDNSLADAGAAYVFVRDESGEWSQTAYLKPDEIDTGDLFGGN
                     VALSGDTLVVGATGEDGVAGDPADNSSNNSGAAYVFERDLAGGWSQSAYLKADNADPD
                     DAFGANPDLEGDVLMVAAIGEQSALPGDPDDNS"
BASE COUNT          855 a         1672 c         1762 g          932 t
ORIGIN      
        1 ccgacacctc gatgcgctcg acctcccggg tcagcacgtg cgagcagatc ccgcagtaga
       61 cgaaattccg ctcgtccttc tcgacgatct ccttgagctt cgggtctagg tcttgacggg
      121 ttttgtcgag gacatccacc atggtgttcg tcgtggctct ttgggttccg cgggatatgg
      181 aatcgttcga tagcatcttg cacagggcgg ttgagcgcaa ggggggcagg gcggccctag
      241 acgcgctgct gcccgcagcc aggtcaggca gcgatctggc cgccttgccg gaccgggact
      301 acctcgccga gatgaccagg tgtgtcttcc gctcgggttt cgtgtggaaa atcatcgaga
      361 acaagtggcc ggggttcgag gttgcattcc gcgggttcga tgtggccggc tgtgcgatgc
      421 tgtccgatga agacatcgag cagctctcgg cggacgagcg gatcgtgcgt cacgcagcga
      481 agatccgttc ggttcctgcc aacgcggcct tcattctgga cgtgcgccgt gaagccggtg
      541 gcttcgggcg ctacctggcc gactggccgg aagacgagat cgtcggtttg tgggacgagc
      601 tcaagcgtcg cggctcgcgg ctcgggggcc agaccggtcg ttactttctg cgtttcgtcg
      661 gcaaagacac gccgatcctg tccagagacg tcgtcaaagc cctgataggt cagggcgtga
      721 tcgatcggga gcccaccagc aagaaagcgc tcgcgcgggt ccaggacgcc ttcaacgaat
      781 ggcacgcgga gagcgggcgc gagctgtgcc agatttcccg ggtcctttcc ctgtccgtgg
      841 actgagcggc cccgggcgaa gtgggcccat aaagaaacgc cggatccaga ggatccggcg
      901 tgaaaagacg cgcgggaagg ggagaggagg agacgatccc gcgcacctgt cgcagaaatc
      961 attgtcatct ttccctgtgc ccatacccat cagggattct acgtatggca cggacggcga
     1021 aggtgtcgat cggccgcctt tgaggctgct caagccagca gggacttcag ctccacatcc
     1081 ggatcgccca gcctggccgc atccaccggc ttgcccacga gctgcttgca caccatgaac
     1141 tctttcgggc tgttcaccgc gtcggcggcc accaccgcgc cgtctttgag gtagaacacg
     1201 gcaaactggt tggctgcctt gtcgccccgg accacctgcg tgtcgccgtc ggcggagaag
     1261 ccgaccatct ggagtttcag ctcgtactgg tccgaccaga accatggaat cgactcgtac
     1321 accttgtctg cgccgcacat gttcgcggcc gctacccggg cctgctccat ggcattgggc
     1381 acggattcca ggcgcaggcg ccggtggagc agcgggttgg gatgattggt gcagtcgccg
     1441 gcggcgaaca cgtcgtccgc cgacgtgcgg cagccttcgt ccacgacgat gccgttgtcg
     1501 cactcgatcc cggcggcctg ggcaacctcg acgttgggga tgatgccgat gcccacgacc
     1561 acgatgtcgg cggcaatctc ctcgtcgccg cactgcactg cgccgacgcg gccctcgccg
     1621 gtgaagccgc tcaccatggt ggaggtatgg atgtgcaccc cccggccggt gtgcagctgg
     1681 tggtaatagt cggacatctc gggcgttgtg accctctgca ggatgcgctc ttccatctcc
     1741 agcacgtgga cctccaggcc cgcctggatt cccactgacg ccacctcgag cccgatatag
     1801 ccgcccccga cgatgacgag ccggcgccct tcagccagct ccgcgcgaat gccgtccacg
     1861 tcggcaatgg tccgcaggta gtgaatgccc gcaaggtcgc tgccgggtac gttgagaatc
     1921 ctgggccgac tgcccgtggc gagcaggagc ttctcgtagc cgatggtttc gccgtcgtcc
     1981 gtggtcacgg tgtgtgcctg ggtgtctatc gcggtgacgc tgacgcccat gcgcagggta
     2041 atttccttgt ccgcgtagaa cttttcgggg cgcaggtaca ccctttccag gccctgctct
     2101 ccggccaggt actgcttgga caggggtggc cgctggtagg gtatgtgcgg ctcgtccccg
     2161 atgatggtga tctcaccttc gtaaccctcc tggcgcaggc tggcagccgc ctgccccgca
     2221 gcatgtccgg cccccacgat aaccatcccc gtcatttgct cagctccttg gttcgtccgc
     2281 tcgccccggc ccgtgcgccg gcccgcgctg gaggggcatc ttactaaggt tcccttgctc
     2341 aggacgagcc gaaaccggga ttgtcgatgg atttgtccgg cgcaggcgtt agcatccgcg
     2401 gtcgagcatc gaaggtggaa atcgcgatga ggtatcggac gctgcatgag atcgtgaggc
     2461 aggcccagcg caatctggat cgcgactgcc gggactacct cctcggcggc gcggacaccg
     2521 agtcttcgct gcggcgcaac cgccatgggc tcgactcctg ggtctttcgt ccgcgtatcc
     2581 tgaacgacgt cagcgaggtg aatctgacca tcgagctgct gggcgcgccg atgcgcatcc
     2641 cggtggttct tccgcccatc ggttcggtgc agctgttcga gccggggggc ggccagacgg
     2701 ttgccgaggc ggcgcacgag ttcggcatcc tccagatcct gagctccgtg tgcacgcccg
     2761 atttcgaggc cgtggccgcc gcggtgcctg gacccaggat ctaccagctc tacctgatgg
     2821 gcgatcgggt ctggctggac gacatgatcg gccggaccat cgaagccggg tacacgggct
     2881 tctgcctgac ggccgacacc cagatctact ccaggcgcga gcgggacatt ctcaagggct
     2941 tcgtgccccc gtcgggcagc caggccggca gccgggactt caactaccag gcgcgaatga
     3001 cctgggagac ggtggcgcac atcaagagca cgttcgacat accgctcatc gtcaagggcg
     3061 tcaacgcggg cgaggacgcg gcgcgctgcg tggaggcggg tgtggacgtc gtttacgttt
     3121 cgaaccacgg tggtcgtcag ctggaccaca ccagggcctg catcgacgcg ctgccggagg
     3181 tcgtcgaggc cgtggccggc cgcgtgccgg taatcgtcga cggcggtttt ctgcgtggtg
     3241 ccgatgtggt caagggcctg tgcctggggg ccgacgtggt cgggatgggt cgtctcgagg
     3301 gcctcgccat ggcggccgga ggacgtgggg ccctcgttcg ggcgctggag atcgtggagc
     3361 aggaaatccg gaccaccatg gcgcttctgg gcgtctccga tcttgccgat ctcaatccgg
     3421 gcctcatgga gcgggctcag cctctggcgg agtccacggt tttcagcgcg ttcccgctgc
     3481 tggaagaggg gtattgagcc cctgcaacca agggaagtga cctagtttca aatacgtcaa
     3541 gatatgttac ggttacaccg tcgccacttg ggcagtttat ttgcggaagg tccggtcatg
     3601 gcacctacat tgcgaacgtc tatccgctgg tttggaacgt ttctcatcgt cttcaccatg
     3661 gcggcatgcg gtggcggagg aggcggggac gatgcgccac gcccgccggg cacggatgcc
     3721 agcctgtccg ggatgagcct gtcgtcgggc agcctggaac cggcgtttgc agcggctacg
     3781 acggaataca cggccgacgt gcccttcgcg gtggccgaga tcacgctgac ggcgacgacc
     3841 agcgactccc gcgcgtctct cacggtagac ggtgcgaccg cctcatccgg aagccccagc
     3901 gacccggtcg cgcttgccgt cggggaaaca aaagtggacg tcgtcgtcac ggccgaggac
     3961 ggaacgacga cccgtgctta ttcgatcact ctcacccggg cggccgccgc gaccaatgcc
     4021 gacctggtct ccctctcctt gagcgccggc gctctggatc agatcttcga cacgacccag
     4081 ccggactaca cggccaccct cgggtacctg gccgcatcgg tgctggtgct ggcagagccc
     4141 gaggatccga acgccgtggt caccgtcaac gccgggaccg ttgacgaacg ggttgcgctc
     4201 gatgagggcg tgaacgtgat cgacttgctg gtgacggcgg aggacggcac caccacccgc
     4261 gccttcgagc tgacgatcac ccggggcgct gcgttggatt tcgcgcagcg gctgtacgtc
     4321 aaacccgacg tcatccgcga gagttaccgt ttcggcgcgg ccgtggacct ggaaggttcc
     4381 tggcttgcgg tcggcgcgcc cggagatcag agccttgcca acaccatcga cggggacgat
     4441 ggcgacttcg ccggcgtgaa cgtgggcggg gtcaccgtct tccaggcaga cgcctcgggc
     4501 ctctggaccc gccaggccta cctcaaggcc gccaatgcag gggatggtga cggcttcggg
     4561 gaggctctcg ccatgtccgg caacaccctt gcagtggggg cccctctcga gcagggcctg
     4621 gacggcgccc cggatgacga cagcggaaac ggggtgggcg cggtgtacgt tttcgtgcgc
     4681 gacgaaagcg acgtctggac ccagcaggcc tacctcaagg cgagcaacgc cgacaacggg
     4741 gatgagtttg gccgttacct cgacctcgac ggcgacaccc tggtggtggg cgcatggcac
     4801 gagcagagcg cgggagccga ggacgacaac tccctggccg acgcaggcgc ggcctacgtg
     4861 ttcgtccgcg acgagtccgg cgagtggtct cagaccgctt acctgaagcc cgacgagatc
     4921 gacacgggcg atctcttcgg tggcaacgtc gcgctttccg gcgacacgct ggtggtgggg
     4981 gctaccggcg aggacggggt ggccggcgac ccggccgaca acagctcgaa caactccggc
     5041 gccgcctacg tgttcgaacg cgacctggcg gggggctggt cccagagcgc atacctcaag
     5101 gctgacaacg cggacccgga cgacgccttc ggcgcgaacc cggacctcga gggcgacgtg
     5161 ctcatggtgg cggccatcgg cgagcagagc gccctgccgg gggacccgga cgacaactcg
     5221 g
//
LOCUS       DBNHQR010000124         2395 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_581091, whole genome shotgun sequence.
ACCESSION   DBNHQR010000124 DBNHQR010000000
VERSION     DBNHQR010000124.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_581091"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..948
                     /locus_tag="AC16VR_02720"
     CDS             148..948
                     /locus_tag="AC16VR_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR6010096.1"
                     /translation="MLARAALATLCWLTPMLLRADAVPGGLYAYGPVPPASEVRFDDR
                     RVLVIDETALVGIPLSASPGTHSLTITYASGESVSHQFEVVAKQYPEQRLTITDRKMV
                     NPDPEDLERIRTESARMREQYLSFTEAMQRLRPFHKPVNGVTSSPFGRRRILNDQPRN
                     PHSGLDIAADHGTPVSAPAPARVSLTGDFYFNGKTIFLDHGQGLVTMYCHLSEISVEP
                     GEAVERGATIGLVGATGRATGPHLHWSVSLNGNRVDPELVISALAVGD"
     gene            complement(986..2392)
                     /gene="der"
                     /locus_tag="AC16VR_02725"
     CDS             complement(986..2392)
                     /gene="der"
                     /locus_tag="AC16VR_02725"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010952071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HIR6010097.1"
                     /translation="MVPTVALVGRPNVGKSTLFNKLTRRRDALVADVPGLTRDRRYGR
                     MPAGAGECMLIDTGGLFGEPGALQSALEQQAELALDEADAVLFVTDARDGLVPADEEI
                     AQRLRRLSKPVILVVNKMDGVQADVAMADFAALGFDAVAYVSAVHERGLGTVRELVEE
                     RCPHGPTGEEAADESDAVRVAVIGRPNVGKSTLVNRMLGQERQVVFDEPGTTRDAIEV
                     PFEKDGKRYVLVDTAGVRRKGRVEGVAEKFSVVKTLQAIDLAHVVILVLDAREGVVDQ
                     DLHLLSHAAEAGSGLVVAMNKWDGLSVEERTNARRVLDRKLSFAPWIPVMQISALHGT
                     GVGHLLEEVDRVYKAGELDVSTSVLTRLLLGMTGAHPPPAVRGRSIKLRYAHKAGSHP
                     PRIVIHGNQTEAVPDSYRRYLENGFRKALALVGNPVHIELRTGDNPYAGQRNELTPRQ
                     KARRKRMIQHRKRKAKKR"
BASE COUNT          402 a          819 c          764 g          410 t
ORIGIN      
        1 ggacggccgg tgaccgccat cgcccaggtc gcccctggtg cgaccatcga tgctcatctg
       61 cacgacggcc ggctccgggt ggaggtggtg tccgtgaacg aagaaagcgc gctgcaggcg
      121 gcgcaaaccc acgtggagga gcactagatg ctcgcccgcg ccgcgctggc cacgctgtgc
      181 tggctgacgc cgatgctcct gcgggccgac gcggtgcctg gcggtttgta cgcctacggg
      241 cccgtgccgc ccgcctcgga ggtgcgtttc gacgatcgtc gggttctggt tatcgacgag
      301 accgctctcg tgggcatccc gctgtctgca tcacccggca cgcactcgct gaccatcacc
      361 tatgcgtccg gagagtcggt ctcgcaccag ttcgaggtgg tggcaaagca gtacccggag
      421 cagcgcctga ctatcaccga tcggaaaatg gtcaatccgg acccggagga cctcgagcgc
      481 atacggacag aaagcgcgag gatgcgcgag cagtacctga gctttaccga agccatgcag
      541 aggctgcgtc cgttccataa gccggtgaac ggagtgacct caagcccctt cggccgccgg
      601 cgcatcctca acgaccagcc ccgcaacccg cacagcggtc tggacatcgc cgccgaccat
      661 ggcacccccg tgagcgctcc ggcacctgca agggtcagtc tcacgggcga cttctatttc
      721 aacggcaaga cgatcttcct cgatcacgga cagggactgg tgaccatgta ctgccacctg
      781 agcgaaatca gcgtggagcc cggtgaggcg gtggaaaggg gcgccaccat aggtctggtg
      841 ggcgccaccg ggcgggccac gggaccccac ctgcactggt cggtcagcct aaacggtaac
      901 cgggtcgatc ccgagctggt aatctctgcc ctggccgtcg gggactagcc caccttcggc
      961 accaccgggc gggagaggac gccggtcagc gtttttttgc tttgcgcttg cgatgctgaa
     1021 tcatcctttt gcgacgggct ttctgacggg gcgtgagctc gttacgctgg ccggcataag
     1081 gattgtctcc ggtgcgcagc tcgatgtgca cggggttgcc caccagggcg agagccttcc
     1141 ggaagccgtt ttccaggtat cttcgatagc tgtccggaac cgcttcggtc tggttgccgt
     1201 ggatcacgat ccggggggga tgcgatccgg ccttgtgggc gtagcgcagc ttgatggaac
     1261 ggccacggac tgcgggtggc gggtgggcgc ccgtcatccc gaggagcagc cgggtcagca
     1321 cagacgtgct gacgtcgagc tctcccgctt tgtagacccg gtcgacctct tcgagaaggt
     1381 ggcccacgcc ggtgccgtgc agggccgata tctgcatcac cggaatccag ggtgcaaaag
     1441 acagcttccg gtccagaacc cgtctggcat tcgtcctttc ctcgaccgag agcccgtccc
     1501 acttgttcat ggccaccacc agccccgagc ccgcctcggc ggcatggctg agcaggtgca
     1561 gatcctgatc caccacccct tcccgggcgt cgaggacgag tatgaccacg tgggccagat
     1621 cgatggcctg cagggtcttc accaccgaga acttctcggc caccccctcg acccgccctt
     1681 tgcgccgcac gccggcggta tccaccagaa cgtagcgctt gccgtccttc tcgaaaggca
     1741 cctcgatggc gtcacgcgtg gtgccgggtt catcgaacac cacctggcgc tcctggccga
     1801 gcatgcggtt caccagcgtg gatttcccca cgttggggcg gccaatcacc gcgacgcgca
     1861 cggcatccga ttcgtcggct gcttcctccc cagtcgggcc gtgggggcac cgctcctcca
     1921 ccagctcgcg tacggtgccc aatcccctct cgtgcaccgc agagacatag gccacggcat
     1981 cgaagccgag ggcggcaaag tcggccatcg caacgtccgc ctgcacgcca tccatcttgt
     2041 tgaccaccag aatcaccggt ttggaaagac gcctcaggcg ttgggcgatc tcctcgtcag
     2101 cgggcaccag gccgtcccgg gcatcggtca cgaacagcac cgcgtcggct tcgtcgagtg
     2161 ccaactcggc ctgctgctcg agggccgact gcagcgcgcc cggttccccg aagagccccc
     2221 cggtgtcgat gagcatgcac tcaccggccc ctgccggcat gcgcccgtag cgccgatccc
     2281 gggtcaggcc gggaacgtcg gccaccagag cgtcccggcg cctggtcagc ttgttgaaga
     2341 gtgtggactt accgacgttg ggccggccaa cgagggcgac ggtgggaacc atccc
//
LOCUS       DBNHQR010000125         2156 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_11278, whole genome shotgun sequence.
ACCESSION   DBNHQR010000125 DBNHQR010000000
VERSION     DBNHQR010000125.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_11278"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="AC16VR_02730"
     CDS             <1..640
                     /locus_tag="AC16VR_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010098.1"
                     /translation="RLTFPHGVPMILASLCGLHLLSALVFLVPHYYMSSRHVVAASLT
                     LLVTIPFVLASVHDRWIQRGRGGRLQRWPYPLIAAALVFMAADGLLSVGGASKRYIRE
                     AGAWLQQNVSQDTRLYSNHRKVLFYGGRPLEFEGPPKAPLAANWISERAWRRYDSVAL
                     WERGNDGEDAQVLAEAAELELVASFANDEGDRVLIFSVGPSAAPTRAAPISP"
     gene            complement(672..1811)
                     /locus_tag="AC16VR_02735"
     CDS             complement(672..1811)
                     /locus_tag="AC16VR_02735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007161952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR6010099.1"
                     /translation="MKLAFCLPEYFPYGGLQNNCLRIAESLLARGHSVDLYTRDWQGP
                     VPRGCRVRVVEVRALRNHSKNVAFARKFAQAMEAGRYDGVVGFMKIPSLDVYYAADPC
                     FQTRAKARRFWLYRLSGRYRALVALERSVFEPDAGTEILMLAEREIDNYVRCYATPQE
                     RFHLLPPAVPRDRMAGADALEVRNALRAEFSVGPDEMLLLSVGSGFKTKGVDRTIRAL
                     AALPGRLGASTSLMVVGQGDPGPFERLARRLGVGDRVRFLGGRTDVRRFLFGADLLVH
                     PAYSENTGNVLLEAMVAGLPVLTTANCGYAPRVERAGGGRVVPEPFAQERLNAALSES
                     LERGRLEEWSRNGIDYGRNEDLYSRPETAADIIESVIEVKAAARR"
     gene            1969..>2156
                     /locus_tag="AC16VR_02740"
     CDS             1969..>2156
                     /locus_tag="AC16VR_02740"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6010100.1"
                     /translation="MRFGFFAMPEHFPWENWTLAYDRDIDRMVRAEQLGFDEFWIGEH
                     HSGGYEPVPVPELMIAKAS"
BASE COUNT          393 a          724 c          660 g          379 t
ORIGIN      
        1 ccgcctgacg ttcccgcacg gcgtcccgat gattctggct tctctgtgcg ggctgcatct
       61 actgagtgcc ttggtctttc tggtgcccca ctactacatg tccagtcgcc acgtggtcgc
      121 ggcgtcgctg acgctgctgg tgacgatccc cttcgtcctc gcctcggtgc acgatcggtg
      181 gatccagcgc ggtcgtggcg gaaggctcca gcgctggccc tacccgctca tcgcagcggc
      241 gctggtgttc atggcggccg acggcctgct atccgttggc ggggcgtcca agcggtatat
      301 ccgggaggcc ggcgcctggc tgcagcagaa cgtttcccag gacacccggc tgtacagcaa
      361 tcatcgaaag gtcctgttct acggcgggcg tcccctggaa ttcgaagggc cgccgaaagc
      421 gcccttggcg gcgaactgga tctccgaacg ggcatggcgg cgctacgact ccgtcgcgct
      481 gtgggagcgc ggcaacgatg gggaggatgc gcaggtgctt gcagaggcag cagagctaga
      541 gctcgtcgcg agctttgcca acgatgaagg ggaccgggtg ctgattttct cggtagggcc
      601 ctcggcagcc ccgacccggg cggcccctat cagtccctga aggtgacaag cgcgttggtg
      661 acaccggacg ctcagcgccg cgctgcggcc ttgacttcga tcaccgactc gatgatgtcc
      721 gcggcggttt ccggccggct gtagagatcc tcgttgcgcc cgtaatcgat accgttgcgg
      781 ctccactcct cgagtcttcc ccgctccagg ctctctgaga gcgcggcgtt gagccgttcc
      841 tgggcgaagg gttcgggaac tacccgcccg ccaccggcgc gctccacgcg aggcgcgtaa
      901 ccacagttgg ccgtggtcag cactggcagc ccggccacca ttgcctccag aagaacgtta
      961 cccgtgttct cgctgtaagc cggatgcacc agaagatcag ccccgaacag aaagcgccgc
     1021 acgtccgtac gtccacccag gaagcggacg cgatcgccaa cacccagccg ccgggcaagg
     1081 cgctcgaagg gccctggatc gccctggcca accaccatca gcgaggtcga cgcgcccagg
     1141 cggccgggaa gcgcggccag cgcccggatg gtgcggtcga cgcccttggt tttgaagccc
     1201 gatcccacgc tcagaagcag catttcgtct ggacccaccg agaactcggc acgcagcgcg
     1261 ttccgaacct ccagcgcatc cgcgcccgcc atccggtcac gcggaaccgc gggcggcaga
     1321 aggtggaacc tctcctgcgg cgttgcgtag catcgtacgt agttgtcgat ctcccgctcc
     1381 gccagcatga ggatctcggt acctgcatcg ggttcgaata ccgatcgttc cagggctacc
     1441 aaggcgcgat agcgtccgct tagccggtac agccagaacc gcctcgcttt cgccctcgtc
     1501 tgaaagcaag gatccgcggc gtagtagacg tcgagactcg ggatcttcat gaagcccacc
     1561 acgccgtcgt aacggcccgc ttccatcgcc tgggcgaatt tccgagcgaa cgccacattc
     1621 ttcgagtggt tgcgaagcgc tctgacctca acgacccgta cccgacaccc tcggggaacg
     1681 ggtccctgcc agtcacgggt atacagatcc acagagtgac cccgcgccaa caggctctcc
     1741 gcaatccgca gacagttgtt ctgcagaccc ccatagggaa agtactcggg aagacagaaa
     1801 gcgagtttca cgggggcgct aatgatctcg atgatcgaca tccatgtgca cgcacgatag
     1861 ccgtttcggc tctgaatcgg caatcacagg ccaccctcga agccgcagcc ccgcccatct
     1921 gctaacatcg cccaacctaa atgactggcg caccgacgag gtaggcgaat gaggtttggc
     1981 tttttcgcaa tgcccgaaca tttcccctgg gagaactgga ccctcgccta cgatcgcgac
     2041 atcgaccgaa tggtgagggc agagcaactc ggtttcgacg agttctggat cggcgagcac
     2101 cacagcggcg ggtacgagcc cgtgccggtg cctgaactga tgatcgccaa ggcttc
//
LOCUS       DBNHQR010000126         2407 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1594571, whole genome shotgun sequence.
ACCESSION   DBNHQR010000126 DBNHQR010000000
VERSION     DBNHQR010000126.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1594571"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="AC16VR_02745"
     CDS             complement(<1..256)
                     /locus_tag="AC16VR_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010101.1"
                     /translation="MRALFLLSISLMSISCWQRDDLVPDMAILPELGNAPVQDPAHTE
                     AFEVQLNDVVYQVEPQYDYDLHGLVVSYRHHDGDRMLHRRW"
     gene            416..2368
                     /locus_tag="AC16VR_02750"
     CDS             416..2368
                     /locus_tag="AC16VR_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR6010102.1"
                     /translation="MALYDNLFEPIEVGPVRIPNRIVRSPHGTGLLGEDLIAYHEARA
                     RGGVGMSTIQATGVHPGAPSGIPIFSDSCRPLLEQIAERMAPHGMKMFLQVYHPGAAG
                     GMGNAEAWSASAIPNPLAGVVPIAMTKTMIDDVVASFAAAAKRCCECGLDGVDVHASS
                     GYLIHEFLSPALNQRTDDYGGSLENRMRFMLEVIEAIRSELGDAAFAVGVRLPNHDYV
                     PGGLTPEMNAEIAQRVDPLVDYVSLHMGAYWRFHKLIAPSDDPLGVEMPSNEPITRVV
                     SKPTIVVGRIMTLDHASHIVDTGAADMVSMVRALIADPELVNKARRRDEHKIRPCIGS
                     NMGCVGQLMTAGRISCVVNIAAAREAKVTHDPETKAAAPKRIMVVGGGPAGLEFARTA
                     AMRGHEVELHEAMKVLGGQVTMAASAPHRADLGAITSWLAEEIDGLGVTVHLNSMVEP
                     DMIAERAPDEVVIATGTTPRADGFQVSTPAASVPGYDLPHVYSSWDLLGFGDITHIEG
                     PAVVFDDTGSFEAISVADVLLGAGVPVTMVSRLNAIGETLPYPPVTVGAARERLMSGD
                     FDFVGGHYLRSINPQEVEVGVLYTERSRRIAARTVILVSFNAPNREIAEALTAQGHTP
                     HLIGDVRARSSIMSAIHGAAELGRRI"
BASE COUNT          409 a          792 c          804 g          402 t
ORIGIN      
        1 tccagcgacg gtggagcatg cggtcgccgt cgtgatgccg ataggaaacc accagcccgt
       61 ggaggtcgta gtcgtactgg ggctccacct ggtagacgac gtcgttcagc tggacttcga
      121 acgcctccgt gtgcgccggg tcctgaaccg gcgcattgcc gagttctgga aggatcgcca
      181 tgtccgggac gaggtcgtct cgctgccagc aggaaatgga catcagactg atcgatagca
      241 ggaacagtgc ccgcaccaac cgctccactg agaaatcctc attgggccga ttcgtgcatt
      301 gtccgtaacg ggtgcgacgg agtcgtggac gagaggcacg tttttgcccg ttcctgcggt
      361 tcctgtttga gccaagccgg gctagagtcc agggttgcag agggaggaat acgccatggc
      421 gctttacgac aacctgttcg agcccatcga ggtcggcccc gtgaggatac cgaaccggat
      481 cgtccgctcg ccccacggaa ccgggctgct cggagaagat ctgatcgcct atcacgaggc
      541 gcgcgcgcgc ggcggcgtcg ggatgtcgac catccaggca acgggcgttc accccggggc
      601 accttcgggc ataccgatct tctcggacag ttgccgcccg ctgctcgagc agatcgccga
      661 gcgcatggcc cctcacggga tgaagatgtt tctgcaggtc taccatcccg gcgcggccgg
      721 cggcatgggc aacgcggagg cctggtccgc cagcgccatt cccaaccccc tcgccggcgt
      781 cgtgcccatc gccatgacga agaccatgat cgacgacgtg gtggccagct tcgccgccgc
      841 agcgaagcgc tgctgcgagt gcgggctgga cggcgtcgac gtgcacgcct cgagcggtta
      901 cctgattcac gagttcctct cgccggcgct caaccagcgc acggacgact acggcgggtc
      961 tctggaaaac cgcatgcgtt tcatgctcga ggtcatcgaa gccatccgca gcgagcttgg
     1021 ggacgcggcc ttcgccgtgg gcgtgcgcct gccgaatcac gactacgtcc ccggcggcct
     1081 gacgccggag atgaacgcgg agattgccca gcgcgttgat ccgcttgtcg actacgtctc
     1141 gctccacatg ggggcctact ggcggttcca caagctcatc gcgccctccg acgaccccct
     1201 cggggtggag atgccctcta acgagccgat cacgagggtg gtttcgaagc cgaccatcgt
     1261 ggtcgggcgc atcatgaccc tcgaccatgc cagccacatc gtcgacacgg gcgcggccga
     1321 catggtttcc atggtccggg cgctgatcgc cgatccggag ctggttaaca aggcgcgccg
     1381 ccgggacgag cacaagatcc ggccgtgcat cgggtcgaac atgggatgcg tcggccagct
     1441 catgaccgcc ggacgcatca gctgcgtggt gaacatagcc gcggcccgcg aggccaaagt
     1501 gacccacgat cccgagacca aggccgcggc acccaaacgc atcatggtgg tcggcggcgg
     1561 gccggccggc ctcgagttcg cgcgcacggc tgccatgcgc ggtcacgagg tggagcttca
     1621 cgaagccatg aaggtgctcg gcgggcaggt gaccatggcg gcttcagcgc ctcaccgggc
     1681 ggacctcggc gccatcacga gctggctggc ggaggagatc gacgggctcg gcgtgaccgt
     1741 gcatctcaac tccatggtgg agcctgacat gattgccgag cgggctccgg acgaggtggt
     1801 gatcgcaacc ggaacgacgc cccgcgcgga cgggtttcag gtgtccactc ccgcagcctc
     1861 ggtgcccggc tacgacctgc cccacgtcta ctcctcctgg gacctgctcg gctttggcga
     1921 catcacccac atcgaggggc cggcggtggt gttcgacgac accggctcgt ttgaggcgat
     1981 ctcggtggcc gacgtgctgc tcggcgccgg cgtgccggtg accatggttt cgcgcctgaa
     2041 cgccatcggc gagaccctcc cttacccgcc cgttaccgtt ggcgcggccc gggagagatt
     2101 gatgtccggt gatttcgatt tcgtcggcgg gcactatctg cgctcgatca acccccagga
     2161 ggtggaagtg ggggtgcttt acaccgagcg cagccgacgc atcgcggcgc gcacggtgat
     2221 cctggtgtct ttcaacgcac ccaacagaga aatcgccgag gcgctcacgg cccagggcca
     2281 cacgcctcac ctcattggcg acgtgcgggc gcgcagcagc atcatgtccg ccattcatgg
     2341 ggcggccgag ctggggcggc gcatctgata ctgcggcaat ccacgatagc gggtgccggg
     2401 aggggag
//
LOCUS       DBNHQR010000127         1773 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_581186, whole genome shotgun sequence.
ACCESSION   DBNHQR010000127 DBNHQR010000000
VERSION     DBNHQR010000127.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_581186"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1773)
                     /locus_tag="AC16VR_02755"
     CDS             complement(<1..>1773)
                     /locus_tag="AC16VR_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010103.1"
                     /translation="PAPEEIWTGEADERVDSVELPIDWQPDDPVRLLSARCSHDVQVA
                     ADALFAWHPDGQKVLYLDTDADGSQTLRSFEVATGESNVAAPVSARSLAFAMAPEGSQ
                     LQIAAADAAATSLWVGPLGPDADSWRRIESSPGPWVVPSLDLEKDDGTSWSLYDLRPR
                     LGVWSPDARWLAHTRLPAVGRDPAGSQLVLTRVAAEVSQRSIDVPRGEVRDLHWQPGA
                     QALGLLNDEKLVVVNPETAQAVFLAGVLGVERFMGWSEPGDHLAYLVLAESFPATEAL
                     LPTGHRIRWAPTDRQNIMVAAADGASPRNRFGMMNITAARWGNRTNRLSFWATYLPTV
                     SLLPPGDPAAVLDLDRDRIQWYPTNIAEFAHVGHYYLLSGSYQTAATQYGRALEKITD
                     AEREAFPDLEWSIHLWRAVAHLAGANEEGAARDLRYVRRHVSVPQNEAGALWDDEVLR
                     ALASDRSILGTMLSMGQIEVAIAEVKRIAEDDSDARRVQALCILALIYDSLGQGDAYA
                     ARVSQDLLPATLASQQIPRELADALVEDSLTALVAPGQLRRLSSSAQRALATQLAKLA
                     GEAPAMSSAALLTAAAIVYRESGDV"
BASE COUNT          319 a          591 c          571 g          292 t
ORIGIN      
        1 cacgtcgccg gactcgcggt aaacgatcgc ggctgccgtg agaagcgcag cggaactcat
       61 cgcgggcgct tctcccgcga gcttcgcgag ctgcgtcgcc agcgccctct gcgcactcga
      121 tgacaagcgc cggagttgac ctggcgccac cagagccgtg agagagtctt cgacgagcgc
      181 gtcggcgagc tcccgcggta tctgttggga cgcgagcgtg gcaggcagca ggtcctgtga
      241 aacccgggcg gcgtacgcgt cgccttgacc aagcgaatcg tatatgaggg ccagaatgca
      301 aagcgcctgg acgcgccgtg cgtcggaatc gtcttcagca attcgcttca cctcggcgat
      361 cgcaacctcg atctgaccca tgctgagcat ggtgccaaga atgctgcggt cggaagccag
      421 cgcgcgaagc acctcgtcat cccacagcgc cccggcttcg ttttgcggca cggaaacgtg
      481 cctgcgcacg tagcgtaagt cccttgctgc gccctcctcg ttggcgcctg cgagatgagc
      541 cacagcacgc cagaggtgga tagaccactc gaggtctgga aacgcttcgc gctcggcatc
      601 ggtgatcttc tccaacgccc tgccgtactg cgtggctgca gtctgatacg acccgctcag
      661 cagatagtag tgaccaacgt gcgcgaactc ggcaatgttc gtcgggtacc actggatccg
      721 gtctcgatcc aggtccagca ccgcagcggg gtcccccggc ggcagcagcg acacggtggg
      781 caggtaggtc gcccagaagg acagcctgtt cgttcggttg ccccaacgcg ccgccgtgat
      841 gttcatcatg ccaaaacggt ttcggggcga ggcgccatca gcggctgcca ccatgatgtt
      901 ttgtcggtcc gtcggggccc atcggattcg atgtcccgtg ggcagcagcg cttccgtggc
      961 cgggaacgat tccgcgagga cgagatacgc cagatgatcg cccggttcgg accatcccat
     1021 gaaccgctcc acgcccagca caccggccag gaagacagct tgggcggtct ccgggttgac
     1081 cacgacaagc ttctcgtcgt tcagcaggcc cagcgcctgc gcgccgggct gccagtgcag
     1141 atcccggact tcgccgcgag gaacatcgat ggagcgctgg gaaacctctg cagctacgcg
     1201 ggtaagcacc agctgagagc cagcgggatc ccgacccacc gccggcagcc gcgtatgggc
     1261 aagccatcgt gcgtccggag accatacgcc gagccgcggt cggaggtcgt atagggacca
     1321 gctcgtccca tcatctttct cgaggtcgag gctgggtacg acccatggcc ccggcgacga
     1381 ctcgatcctt cgccacgaat ccgcgtcagg gccgagtggg ccaacccaca gtgaggtggc
     1441 agccgcgtcc gcggctgcaa tctgaagctg gctgccctcg ggcgccatgg caaaggcgag
     1501 gctgcgcgcg ctcacaggcg cagcaacatt ggattcgccg gtagctacct caaaggatcg
     1561 cagcgtctgg gaaccgtcgg cgtcggtatc gagatacagc accttctggc catcggggtg
     1621 ccaggcaaac aaggcatcgg ccgccacctg cacatcgtgg ctgcaccgcg cgctgagaag
     1681 cctcacgggg tcgtcgggct gccagtcgat gggcagctcg acggaatcga ccctctcgtc
     1741 agcctcgccc gtccagattt cctccggcgc ggg
//
LOCUS       DBNHQR010000128         4533 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_581236, whole genome shotgun sequence.
ACCESSION   DBNHQR010000128 DBNHQR010000000
VERSION     DBNHQR010000128.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4533)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_581236"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="AC16VR_02760"
     CDS             complement(<1..880)
                     /locus_tag="AC16VR_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017679291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR6010104.1"
                     /translation="MGGLSTASFWENFLGQAPRWYKTTIVGFLIVNPFLFMISEFVTG
                     WILVLEFIFTLAMALKCYPLQPGGLLAIEAVAIGMTSTDTIREEVFNNFPVILLLMFM
                     VAGIYFMKELLLFTFTKIILGVRSKTLVSLLFCAVAAILSAFLDALTVTAVIISVAVG
                     FYSVYHRVASGKSFRDEHEHGADESIGELHREDLESFRAFLRSLLMHGAVGTALGGVC
                     TTVGEPQNLLIAGQVGWEFMEFFLRMAPVTMPVLVVGLTTCVVLEKARAFGYGTDLPG
                     PVRDILERFDQAEAEAR"
     gene            1142..1993
                     /gene="nudC"
                     /locus_tag="AC16VR_02765"
     CDS             1142..1993
                     /gene="nudC"
                     /locus_tag="AC16VR_02765"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HIR6010105.1"
                     /translation="MWPIYPDEFESLVQAPPGEEDDRDAWYFLCINGQIACVVDRGAP
                     RPITADEFRWLDIEVFSKHYLGRFGNRPCFAMEGRGQLPPDGYLADSLYSWLGRVEPS
                     VFYLAGRAQQVVEWHNTHQYCGRCGAEMQEHAADRAKTCGGCGLICYPRIAPSIIVLV
                     TKGDEMLLARNARWPTGMYSTLAGFVEVGESIEQTVHREVYEEVGIQVDNLRYLGSQS
                     WPFPNSLMLGFHADYAGGEIICNEEEIADAKWFAHDELPSVPPATAISRWLIDDFIEE
                     VGRRSSG"
     gene            complement(2002..2349)
                     /locus_tag="AC16VR_02770"
     CDS             complement(2002..2349)
                     /locus_tag="AC16VR_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HIR6010106.1"
                     /translation="MSDSTMPHDDLTRRLQSAFHPPAASHMDATFALDLGQLRISFRV
                     RQGRLDFVEGVPADATFYFDTPDEALQLLTGDADVFEAFMEGRFRADGYLMWTFALLS
                     MFRGDGAEPTPTG"
     gene            2466..3506
                     /gene="sohB"
                     /locus_tag="AC16VR_02775"
     CDS             2466..3506
                     /gene="sohB"
                     /locus_tag="AC16VR_02775"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019470684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HIR6010107.1"
                     /translation="MEYLLDYLLFLAQAVTVVVAVLVVLSGVASVSARFQGAERGGHL
                     EVKKLNDRLEELKSGLQQALLDSSTYKKQRKRDAKAAKQRAKVERAEAAKADSEHRKR
                     VFVLDFHGDLQASAVHDLTTELTAVLTLADDRDEIVVKVESPGGLVHGYGLAASQLDR
                     VREHGVPLVVAVDKVAASGGYLMAAVADRVVAAPFAVVGSIGVVAQVPNVHRLLKKHD
                     VDVEVLTAGKFKRTLTVLGENTEEGRKKFIEDLEDTHALFQEFVAARRPQLDMATVAT
                     GETWHGQRAMDLKLVDELCTSDEYLVRSCETADVFSVRWVEHKKPLERIMAKVETGVQ
                     NIVDQLFRRPGA"
     gene            3503..4495
                     /locus_tag="AC16VR_02780"
     CDS             3503..4495
                     /locus_tag="AC16VR_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016253557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HIR6010108.1"
                     /translation="MTGFRALLVEKTETGFQRSVVTRRVEDLPPGDLLIDVRYSSLNF
                     KDALSATGNPGVSRNFPHTPGIDAAGVVVESSSEGFAPGDAVVAIGFDLGMNTPGGFG
                     QRIRIPAGWALPLPEGLDLRESMRLGTAGFTAALCVQKLEEAGMTPDGGPVLVTGATG
                     GVGSVAVMLLAKLGYRVTAVTGKPDQHGFLESIGAAEIVSREEARSGSDRPLLPETWG
                     GVVDTVSGETLFNSIKRLRYGASLAACGLVESPQFAATVLPFILRNVNLLGVDSVELP
                     LAEKARVWDKLAGPWKLEGLESLEETLSLDTVSDAIDRILAGAMVGRGVVDLYA"
BASE COUNT          829 a         1368 c         1515 g          821 t
ORIGIN      
        1 tgcgtgcttc agcttcggcc tggtcgaagc gctcgagaat gtcgcgcacc gggccgggca
       61 ggtcggttcc gtagccgaaa gctctggctt tttctaacac gacgcaggta gtcagcccga
      121 ccaccagaac cggcatcgtg acgggtgcca tgcgcaggaa gaactccatg aactcccatc
      181 ccacctggcc ggcgatcagc aggttctgtg gttcgcccac cgtggtgcag acaccgccca
      241 atgcggttcc cacggcgccg tgcatcagca ggctgcgcag gaaggcgcgg aacgactcca
      301 ggtcctcacg atgcagctca ccgattgatt cgtccgcacc gtgctcgtgc tcatccctga
      361 aggactttcc ggaggcgacc ctgtggtaga cgctgtaaaa gcccacggca acgctgatga
      421 tcaccgccgt caccgtcagc gcgtcgagaa aggcggacag gatcgcggcg acggcgcaga
      481 acagtaagga gacgagggtc tttgagcgaa ccccgagaat gatcttggtg aaggtgaaga
      541 gcagcagctc cttcatgaag taaatgcccg ccaccatgaa catgaggagc aggatgacgg
      601 gaaagttgtt gaagacttcc tcgcgtatgg tgtccgtgga cgtcatgccg atcgccacgg
      661 cttcgatagc gagcagaccg ccgggttgca gcggataaca tttcagggcc atggccaggg
      721 tgaagatgaa ctcgaggacc agaatccacc cggtgacgaa ctcgctgatc atgaaaagga
      781 acggattgac gatcaggaaa ccgacgatgg tcgttttgta ccaccgtggt gcctggccga
      841 gaaaattttc ccagaagctg gccgtggata gtccgcccat ctgcttttgc ccggcgcgcc
      901 gggaaaactg ctccctgtaa ttgcctgcga ggtggccgca gcaaaccagc agacctttgc
      961 ctccccggcc ggtcctccct tccggccaaa ccggtggtcg agcgcacggg tgtcagatca
     1021 cgcttcgaga acattatccg gctcggcagg ggagttcaat acgatcgtgg ccggaccctg
     1081 aacaggccgg tgggtgtgtt atcgtttcgg ctcatcgtcg ataccacgag tcttgcccgc
     1141 catgtggccc atctaccccg atgagttcga gtctctcgtt caagcccctc ccggtgagga
     1201 ggacgatcgg gacgcctggt acttcctttg catcaacggt cagatcgcct gcgtcgtgga
     1261 ccgcggcgcc ccccggccga tcactgccga tgagttccgg tggctggaca tcgaggtctt
     1321 ctccaagcac taccttggcc gcttcggcaa tcgcccctgc ttcgctatgg aggggcgcgg
     1381 ccagctgccg ccggacggtt acctcgcgga cagcctttac tcgtggctag ggcgcgtaga
     1441 gccgtcggtg ttctacctgg cgggtcgggc gcagcaggtg gtggagtggc acaacaccca
     1501 tcagtactgc gggcgctgcg gcgcggagat gcaggaacac gccgccgatc gtgccaagac
     1561 gtgcggcgga tgcgggctga tctgctatcc gaggatcgcg ccgagcatca tcgttctggt
     1621 gacgaaaggc gacgagatgc tgctggctag aaacgcgcgt tggccgacgg gcatgtacag
     1681 cacgctggcg gggttcgtcg aggtgggcga gtccatcgag cagacggtgc atcgggaggt
     1741 ctacgaagag gtcggaattc aggtagacaa tcttcgctac cttggcagtc agagctggcc
     1801 gtttcccaac tcgctcatgc tgggctttca cgcggactac gcaggcggcg agatcatctg
     1861 caacgaggag gagatagccg acgccaagtg gtttgcccac gacgagctgc ccagcgtgcc
     1921 ccctgctacc gccatctccc gatggctcat cgatgacttc atcgaggaag tcgggcgccg
     1981 cagctccggc tgatgcggcc ttcagccggt tggtgtgggc tctgccccgt ccccgcgaaa
     2041 catgctcagc agggcaaaag tccacatcag gtagccgtcc gcgcgaaagc ggccctccat
     2101 gaaggcctcg aacacgtccg catcgcccgt gagcagctgc agcgcctcat ccggcgtgtc
     2161 gaagtaaaag gtcgcatctg ccgggacgcc ctccacgaaa tcgagccggc cctgacggac
     2221 gcgaaaacta atgcggagct ggcccagatc gagggcaaac gtggcgtcca tgtgggaagc
     2281 cgccggcgga tgaaacgcgc tctgcagtcg ccgggtcagg tcgtcgtggg gcatcgttga
     2341 atcagacacg catgtgcgga tgagatcaag ccgtgcgcat gtggctgcag gcgcggcggt
     2401 ggacgcccgt tgacgcagcc agtacattag cgcgcccttg ctttgcgtaa aaattccggg
     2461 gtggcatgga gtatctgctc gactatctgc tctttctggc ccaggccgtc acggtcgtcg
     2521 tggccgttct cgtcgtgctt tccggcgtcg cttccgtgag cgccaggttc caaggtgcgg
     2581 agcggggcgg tcatctcgag gtcaagaagc tcaatgaccg gctcgaggag ctgaagtcgg
     2641 ggctgcagca ggctctgctc gacagctcga cctacaaaaa gcagcgcaag cgggatgcca
     2701 aggccgccaa gcagcgagcc aaagtcgaga gggccgaggc tgccaaggcc gatagcgagc
     2761 atcgcaagcg ggtcttcgtc ctcgacttcc acggcgatct ccaggcgagc gccgtccatg
     2821 atctcacgac ggagctgacc gctgtgctca cgctcgccga tgaccgtgac gagatcgtgg
     2881 tcaaggtgga aagccccggc gggctggtgc acggatacgg cctggcggct tctcagctcg
     2941 accgggtgcg tgagcacggg gtgccgctcg tcgtcgcggt ggacaaggtc gccgcgagcg
     3001 gtggttatct gatggctgcc gtggctgaca gggtggtcgc ggccccgttt gcggtcgtgg
     3061 gatccatcgg cgtggtggcc caggtaccca acgtccatcg gcttctgaaa aagcacgacg
     3121 tggacgtgga ggtgctcacg gccggcaagt tcaagcgcac gttgaccgtg cttggtgaga
     3181 atacggaaga gggccggaag aagttcatcg aggacctcga ggacacccac gcgctgtttc
     3241 aggaattcgt ggccgcgcgg cgtccgcagc tggacatggc caccgtggcc accggagaga
     3301 cctggcacgg gcagcgggcc atggacctca agctggttga cgagctttgc accagcgacg
     3361 agtacctggt gcgctcgtgc gagacggcgg atgtgttcag cgtgcgctgg gtggagcaca
     3421 agaagccgtt ggaacgaatc atggccaaag tggagaccgg cgttcagaac atcgtcgatc
     3481 agctgttcag gaggcccgga gcatgacggg tttccgtgcc ctgctggtgg agaagaccga
     3541 aaccggtttc cagcgctcgg tcgtcacgcg ccgcgtggag gatttgccgc ccggcgatct
     3601 gctgatcgac gttcgctact cctcgctcaa cttcaaagat gcgctttccg ccaccggcaa
     3661 tccgggcgtg agccgtaatt ttccgcacac cccgggcatc gacgctgcgg gcgtggtcgt
     3721 ggaatcgtcc agcgagggct tcgccccggg cgacgccgtg gtcgccatcg gtttcgacct
     3781 cggcatgaac acgccgggcg gtttcggcca gcgcatccgg attccggcgg gatgggccct
     3841 gccgctaccg gagggattgg atcttcgcga atccatgcgc ctcggaacgg ccggtttcac
     3901 ggccgccctg tgcgtgcaga agctcgaaga ggcgggcatg acgccggatg gcggtccggt
     3961 gctggtgacc ggcgccaccg gtggcgtggg atccgtcgcc gtcatgctgc tggcaaagct
     4021 cggctatcgg gtcaccgcgg tgacgggcaa acccgatcag cacggctttc tcgagtccat
     4081 cggcgctgca gagatcgtgt cccgggaaga agcgcgctcc gggtcggaca ggccgttgct
     4141 gccggaaacc tggggtgggg tggtggacac cgttagcggc gagacgctct tcaactcgat
     4201 caagcgcctg cgttacgggg cgagcctggc cgcttgcggt ctcgtggagt cgccgcagtt
     4261 cgcggccacc gtgctgccct tcatcctgcg caacgtcaat ttgctcggcg tggattcggt
     4321 tgagctgccc ctggcggaga aagcgagggt ctgggacaag ctggcgggcc cctggaagct
     4381 cgaaggttta gagtccctcg aggagacgct ctctctggat accgtctcgg atgccatcga
     4441 ccggatactc gccggtgcca tggtgggacg tggcgtggtg gacctgtacg cctgaaactc
     4501 agtccctgcg cagccgggcc gatattgcaa tcg
//
LOCUS       DBNHQR010000129         1738 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1531216, whole genome shotgun sequence.
ACCESSION   DBNHQR010000129 DBNHQR010000000
VERSION     DBNHQR010000129.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1531216"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="AC16VR_02785"
     CDS             <1..314
                     /locus_tag="AC16VR_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010109.1"
                     /translation="EEFASLKRNMELRLPGRFETLASLEDAAHDLVNYGYSPAFYYDY
                     AKTVRALGEQDLAEAVNRVINPEQLIWVVVGDLEKIEAQVRALGFGEVVRLKASGASG
                     D"
     gene            383..1441
                     /locus_tag="AC16VR_02790"
     CDS             383..1441
                     /locus_tag="AC16VR_02790"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6010110.1"
                     /translation="MADDYRERMQAYAQRLMGYKTGEMVSLMVHIGDELGLYRAMAGQ
                     DSVTAESLAEATGLHERWVAEWLRGQGAAEILDHVGDERFRLSDVAEQALLNEDSPAY
                     LQGFFSRPPSHEIVDRTIEAFSTGVGVSWGAHGDSASHFLCRTNRPFHMKLADEVIPL
                     LGGMSERLEAGGAVLDVGCGPGTALTELARAFPNSQFVGVDPAANMIAQAQQRYADMD
                     NVRFEVGYGETLEDVAAYDLVTTFDCMHDMTDPEGTMRAVRRAVKGDGAWLIKDIKAQ
                     DSYAENLENPVAAMMYGFSVLFCMSSALSKPGGAGLGTLGFPPSVCERMGHDAGFGQF
                     RILDFEGDPFNFYYELRP"
     gene            complement(1467..>1738)
                     /locus_tag="AC16VR_02795"
     CDS             complement(1467..>1738)
                     /locus_tag="AC16VR_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010111.1"
                     /translation="NLPVSFLDEHGEALRDRYYQQLQSNGVQGYARADLEADLAIGTM
                     AMTGIPIIGGANADLSNPRNEALFTEVGSRAFGAVIGNDCLAYLP"
BASE COUNT          308 a          505 c          609 g          316 t
ORIGIN      
        1 gagaggagtt tgcgagcctc aaacgaaaca tggagctgag gttgcccggg cgtttcgaaa
       61 cgctggcgtc actggaagac gcggcgcacg atctggtcaa ctacgggtac tcgccggcct
      121 tctactacga ctacgccaag accgtgcggg cgctcggcga gcaggatctg gccgaggcgg
      181 tgaaccgggt gattaaccct gagcagctca tctgggtggt agtcggcgac ctggagaaga
      241 tagaggcgca ggtgcgcgca ctagggttcg gcgaggtggt caggctcaag gcgtcgggtg
      301 cttccggcga ctgagcgctg gcttgctgcg ggatgcggcg gcgaaacgcc gcggggatac
      361 aacggtaacg gggaaacttg ccatggcgga cgattatcgg gaacggatgc aggcctacgc
      421 ccagcgtctg atgggctaca agaccggcga gatggtgtct ctgatggtgc acattggcga
      481 tgagctcggc ctttaccggg ccatggcggg acaggacagc gtgacggctg aatccctggc
      541 cgaggccacc ggcctgcacg agcggtgggt tgccgaatgg ctgcgcggcc agggcgccgc
      601 ggagattctc gatcacgttg gcgacgagcg gttcaggctg tcggacgtgg ccgagcaggc
      661 gcttctgaac gaggacagcc ccgcgtatct gcagggcttc ttctcccggc cgccgtccca
      721 cgagatcgtc gaccggacca tcgaggcttt cagcacgggt gtgggggtca gctggggcgc
      781 ccacggcgat tcggcctccc attttctctg ccggaccaat cggcctttcc acatgaagct
      841 cgcagacgag gtgatccccc tgctgggcgg aatgtcggaa cgactcgaag ccggtggtgc
      901 cgtcctggac gtgggttgcg gtcctggcac ggcgctgacc gagctggcaa gagcctttcc
      961 caactcgcag ttcgtaggtg tcgatccagc ggccaacatg atcgctcagg ctcagcagcg
     1021 ctatgccgac atggacaacg tgcgcttcga ggtgggctac ggcgagacgc tggaggacgt
     1081 ggcggcctac gacctcgtca ccaccttcga ctgcatgcac gacatgacgg atccggaggg
     1141 aaccatgcgg gcggtgcgca gagctgttaa gggagatggc gcctggctga tcaaggacat
     1201 caaggcacag gacagctacg ccgagaatct ggagaacccg gtggccgcga tgatgtacgg
     1261 tttctcagtg ctgttttgca tgtcatcggc gctctcgaag ccgggcggcg ccgggctcgg
     1321 caccctggga ttcccgccca gcgtctgcga gcgcatgggc cacgatgcgg gcttcggtca
     1381 gttccggatc ctggacttcg aaggcgatcc gttcaatttc tactatgagc tcagaccctg
     1441 acggcgttgc ctgatcggtt cctgaatcaa ggcaggtagg ccaggcagtc gttgccgatc
     1501 accgcgccga aagcccggct gccgacctcg gtgaagaggg cttcgttgcg cgggttggac
     1561 aggtccgcgt tggcgccgcc gatgatgggt atgcccgtca tggccatggt gccgattgcg
     1621 aggtcggcct ctaaatcggc gcgcgcatac ccctggaccc cgttgctctg tagctgttgg
     1681 tagtagcggt cgcgtaaagc ctcgccgtgc tcgtccagaa aagacacggg caggttcg
//
LOCUS       DBNHQR010000130         4593 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1279090, whole genome shotgun sequence.
ACCESSION   DBNHQR010000130 DBNHQR010000000
VERSION     DBNHQR010000130.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4593)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1279090"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="AC16VR_02800"
     CDS             <1..868
                     /locus_tag="AC16VR_02800"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR6010112.1"
                     /translation="GRWLVRPGRSLPGHYYLQRGAVRLREPDQRVAAGSPRSRWPIYP
                     GAQGVLTLSPVTLVRVSEDAAAATAHAREVQPPLSRPQWLTDGWECRFLSSGAMGRVS
                     LDHCQRVMRAMRPVDLLRGEMVVRTGEPGDTVFVLAAGRAEVSRGRRSLALLEPGDHF
                     GEDAAISGACRNACVTMVSDGRVMTLSGELFRDFVVGRVLLDDGDGPAQATIDIGGRS
                     VSAGLGIPLLLLRERLGLLTPELRYRLAGGSLRQRVLAAFILLHRGFRVALAESGLDA
                     EVALHPEPRAHL"
     gene            943..1287
                     /locus_tag="AC16VR_02805"
     CDS             943..1287
                     /locus_tag="AC16VR_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018403973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HIR6010113.1"
                     /translation="MSSDCLFCRIASGEIPGDIVYEDDELLAFNDISPQAPHHVLVIP
                     KIHVATVNDLAAEHADLVGRLVLCARAIAAERNIQESGYRLILNCNGHGGQTVFHLHL
                     HLLGGRQLMALG"
     gene            1289..1927
                     /gene="coq7"
                     /locus_tag="AC16VR_02810"
     CDS             1289..1927
                     /gene="coq7"
                     /locus_tag="AC16VR_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017517343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HIR6010114.1"
                     /translation="MAGPGSLADALVAQVDKALRTLTGAAASVRENPAADVPEGEMSP
                     GEREHAAGLMRVNHTGEICAQALYEGQALTASDPRTRTALLAAASEESDHLAWCAGRL
                     AELGSSPSVLNPAFYALSYALGAVTGVLGDKVSLGFVEATEDQVCRHLESHLQSLPAG
                     DSRSRQIVRAMREDEARHGAEALGAGGAEFPQPVKRAMSLASRLMTETTYRV"
     gene            complement(1915..2328)
                     /locus_tag="AC16VR_02815"
     CDS             complement(1915..2328)
                     /locus_tag="AC16VR_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012274236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6010115.1"
                     /translation="MTKPPKPHAGLRHVALFTTTLEASVRFYTELLGMQVEWNPDPDN
                     FYLTSGTDNLALHRATEGRSVRGQTLDHIGFIIDDEEDVDAWHDHLAANGVNILERPR
                     THRDGARSFYCEDPDGNVVQLIYHPPISRPGGQTR"
     gene            complement(2348..2767)
                     /locus_tag="AC16VR_02820"
     CDS             complement(2348..2767)
                     /locus_tag="AC16VR_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014872825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR6010116.1"
                     /translation="MQAKITLIENVHFEAVADSGHTVLIDGPPDAGGENRGTRPMELV
                     LMGLGGCASFDVVSILRKSRQPVVGCVTRLTARRADTVPQVFEAIHLHFTISGQGLDE
                     AKVARAVALSAEKYCSASIMLERGGVAISHDYEILEA"
     gene            2967..3626
                     /gene="crp"
                     /locus_tag="AC16VR_02825"
     CDS             2967..3626
                     /gene="crp"
                     /locus_tag="AC16VR_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HIR6010117.1"
                     /translation="MGKPLTAKGFNPNAGQEDTHLDAFLAAAHRRKYPAKSTLIYAGD
                     KSDSIYYILKGSVTVLVEDESGREIIVAYLNKGDFFGEMPLFTESAPRSAWIKAKTEC
                     EVAELSYGKFRELAQRHSDLLYAVGRQMADRLGRTTQKVSDLAFLDVTGRVARTLIEL
                     TKQPDAMTHPDGMQIKITRQEIGRIVGCSREMVGRVLKTLVEQGLVNVKGKTMVVYGT
                     R"
     gene            complement(3771..4556)
                     /gene="trpC"
                     /locus_tag="AC16VR_02830"
     CDS             complement(3771..4556)
                     /gene="trpC"
                     /locus_tag="AC16VR_02830"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - L-tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HIR6010118.1"
                     /translation="MTILDEILAHKRTEVAERQRVRSLDSLRALAQACPPARGFADAL
                     ESKLLAGASGVIAEIKRASPSKGVIREDFDPVSIARSYAKGGAACLSVLTDERYFQGH
                     DEYLQAARAAVDLPVLRKDFVVDAYQLFEARAMGADCVLLIAAALDAGQLKALHETAI
                     ALGLDVLVEVHDGEELESALEIEPRLVGINNRNLKTFETRLETTYGLLPGIPDSVTVV
                     TESGIANREDVAAMRQHGVHCFLVGEAFMRAPDPGTALAEMFA"
BASE COUNT          753 a         1469 c         1539 g          832 t
ORIGIN      
        1 gggccgttgg ctggtcaggc caggccggtc gctgcccggg cactactatc tgcagcgagg
       61 agcggttcgt ttgcgggaac ctgaccaacg ggttgctgcc gggtcgccgc gatcccggtg
      121 gcccatctac ccgggcgctc agggcgtgct caccctatct ccggtcaccc tcgtgcgcgt
      181 gagcgaagat gcggcggccg ccacggccca cgcccgggaa gtccagccgc cgctctcccg
      241 gccccagtgg ttgaccgacg ggtgggagtg ccgctttctc agctccggag ccatggggcg
      301 ggtcagcctg gaccactgtc agagggtgat gcgggccatg cgccccgtgg acctcctccg
      361 gggcgagatg gtggtgcgaa ccggggagcc cggcgatacc gtcttcgtgc ttgcggccgg
      421 ccgagccgag gtcagcagag ggcggcgttc gcttgcgctg ctcgagccgg gtgaccattt
      481 cggggaggat gcggccatca gcggtgcgtg ccgcaacgct tgcgtgacca tggtctccga
      541 cggtcgcgtg atgacgctct ccggtgagct gtttcgcgac ttcgtggtcg ggcgggtgct
      601 tctcgacgac ggcgatggcc ccgcccaggc gaccatcgac ataggcggac ggtccgtgtc
      661 ggcgggcctg ggtatacccc tcctgctgct cagggagcgg ctcgggttgc tgacgcccga
      721 gctgcgctac cgtctggcgg gtggcagtct ccggcagcgc gtgctggctg ccttcattct
      781 ccttcatcgc ggctttcgcg tggcgcttgc cgagagcggg ctggacgccg aagtcgcgct
      841 ccatcctgag ccgcgcgctc atctttaagc ccgcgttgcg ggggctgttg tgccggttta
      901 cagtcacctc tcccggcatg ttgaccgaag cgcgagaagc gaatgagttc cgattgcctg
      961 ttctgcagaa tcgcgtccgg cgagataccc ggcgacatcg tctacgagga cgatgagctg
     1021 ctcgccttca acgacatcag cccgcaggcg ccgcatcacg tgctcgtcat cccgaagatt
     1081 cacgtcgcca ccgtgaacga ccttgcggcc gaacacgccg atcttgtcgg tcgtctggtg
     1141 ctctgtgccc gggccatcgc cgccgagcgc aacattcagg agtcgggcta ccggctgatc
     1201 ctcaactgca acgggcacgg cggccagacg gtcttccacc ttcacctcca cctgctgggc
     1261 ggccggcagc tgatggcgct gggctgacgt ggctggtcct ggctctttgg ctgatgccct
     1321 ggtggcgcag gtagacaaag cgctgcgcac gctgacgggc gctgcagctt cggtccgtga
     1381 gaatcccgcg gccgatgtgc ctgaaggaga gatgagccct ggcgagcggg agcacgctgc
     1441 cggcctgatg agggtgaatc acaccggcga gatatgcgcc caggcgctct acgagggcca
     1501 ggcgctgacg gcgtcggacc cccgcacccg gacagcgctg ctcgcggcag cctccgaaga
     1561 gtcggaccac ctcgcctggt gtgccggccg gctcgccgag ctgggcagca gcccgagcgt
     1621 gctcaacccg gccttctacg cgctttccta tgccctgggg gcggtgacgg gcgttctcgg
     1681 tgacaaggtc agtctgggat tcgtggaggc gacggaggat caggtctgcc gacacctcga
     1741 gtcgcacctg cagtccctgc cggccggcga ttcccgcagc cgtcagatcg ttcgcgccat
     1801 gcgggaagac gaagcgcgcc acggggccga ggccctggga gccggcgggg ccgagtttcc
     1861 ccagccggtg aaacgggcga tgtcgctggc gtccaggctg atgaccgaga cgacctaccg
     1921 cgtctgacca cccggtcggg aaatcggcgg atgatagatg agctgcacca cgttgccgtc
     1981 ggggtcctcg cagtagaacg atcgcgcacc gtcgcgatgg gtcctgggtc tctcgaggat
     2041 gttcacgccg ttggcggcca ggtggtcgtg ccaggcgtcc acatcttcct cgtcgtcgat
     2101 gatgaagccg atgtggtcca gggtctggcc gcgcacggag cggccctcgg ttgcccggtg
     2161 cagcgccagg ttgtccgttc cggacgtgag gtagaagttg tcgggatccg gattccactc
     2221 cacctgcatg ccgaggagct cggtgtagaa gcgcacggag gcttccagcg tggtggtgaa
     2281 cagcgccacg tgccgcagcc ctgcatgcgg cttgggtggt ttggtcatct gcggaggttc
     2341 cggagcttca tgcttccagt atttcgtagt cgtggctgat tgcaacaccg ccgcgctcga
     2401 gcatgatcga agcggagcag tacttctccg ccgacagcgc cacggctctt gcgaccttgg
     2461 cctcgtccag gccctgcccg ctgatggtga agtgcagatg aatggcctcg aacacctggg
     2521 gtaccgtgtc ggcgcgccgg gctgtgagcc tggtcacaca gccgacgacg ggctgccgcg
     2581 acttcctgag aatgctgacc acgtcgaacg acgcgcagcc gcccagaccc atgagcacga
     2641 gctccatcgg ccgggtacct ctgttctcgc cgcccgcgtc cggcggaccg tcgatgagca
     2701 ccgtgtgccc ggagtctgcc acggcttcga aatgaacgtt ctcgatcagc gtgatttttg
     2761 cttgcatggc cggcagtttc cgatatctgg atgcccgata cgtgaggctg ggagcgtctg
     2821 agtagtgatg ggggaccact catactgtga aatggatacg tttgtcgacc agttctcgtt
     2881 gtggttgtaa acgatatgca agtattcatc cgcaaggttc acaaggagcg gaaatgcttg
     2941 ccaacaagaa ttcacagttc tcagggatgg gaaaaccact gaccgccaag gggttcaacc
     3001 cgaacgccgg gcaggaagat acgcatctgg atgcctttct ggcagcggcc catcgccgca
     3061 agtatccggc caagagcacg ctcatctacg ccggtgacaa gagcgattcg atttactaca
     3121 tcctcaaggg ctcggtaacc gtgctcgtgg aggacgaatc cggacgtgaa atcatcgtcg
     3181 cctatctcaa caagggcgat ttcttcggcg agatgccgct tttcacggaa agcgcgccgc
     3241 gttcggcctg gatcaaagcc aagaccgagt gcgaagttgc cgaactcagc tacggcaagt
     3301 tcagggaact cgcccagcgc cacagcgatc tgctctacgc cgtgggccgg cagatggccg
     3361 atcggctcgg ccgcactacc cagaaggtca gtgatctggc gttcctggac gtgaccggcc
     3421 gcgtcgcgcg tacgctgatc gagctcacca agcagcccga tgccatgacc catcccgatg
     3481 gcatgcagat caagatcacc cgccaggaga tcggccgaat cgtcggatgc tcccgcgaga
     3541 tggtggggcg tgttctgaag acgctggtcg agcagggtct ggtgaacgtc aaaggcaaga
     3601 cgatggtggt ttacggcacc cgctaagacc ccagcaggcg cgccgaaagg aaacggcgcg
     3661 cccggttgct cacacaggac gtggaacagc gggctctctg ccagatccgg cggagagccc
     3721 gttttccgtt tctgctgcag ggacgcggcc gagggtcgag cccggcggtc ctaagcgaac
     3781 atctcggcga gcgccgtccc cggatccggc gcgcgcatga acgcttcgcc gacgaggaag
     3841 cagtgcacgc cgtgctgccg catggccgcg acatcctcgc ggtttgcgat gccgctctcc
     3901 gtcaccaccg tcacgctgtc gggaatgccc ggcaggagtc cgtaggtggt ttcaagccgc
     3961 gtctcgaagg tcttcaggtt gcggttgttg atgccgacca ggcgaggctc gatctccagg
     4021 gccgactcga gctcctcccc gtcgtggacc tccacgagca cgtccagacc gagcgcgatg
     4081 gccgtttcgt gcagggcctt gagctgtccc gcgtcgaggg cggccgctat gagaagcacg
     4141 cagtccgcgc ccattgcgcg tgcttcgaac agctgatatg cgtccaccac gaaatccttg
     4201 cgcagcacgg gcaggtccac ggccgctcgg gcggcctgca ggtactcgtc gtggccctgg
     4261 aagtagcgct cgtcggtaag aacggagagg caggcggcgc cccccttggc gtagctccgg
     4321 gcaatggaca cggggtcgaa atcttcccgt atgacgcctt tgctgggcga tgctctcttg
     4381 atctctgcga tcacgcctga agcgccggcc aaaagcttgc tttccagcgc atctgcaaag
     4441 ccgcgggcgg gcgggcacgc ctgggccagg gcgcgcaggg aatccaggct tcgcacccgc
     4501 tggcgctcgg ccacctcggt gcgtttgtgg gcgagaatct cgtcgagaat ggtcacggcg
     4561 ccgccaccat cacggctctt ctaccgtcac ggc
//
LOCUS       DBNHQR010000131        20892 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_12099, whole genome shotgun sequence.
ACCESSION   DBNHQR010000131 DBNHQR010000000
VERSION     DBNHQR010000131.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 20892)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 20892)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20892
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_12099"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /gene="ccsA"
                     /locus_tag="AC16VR_02835"
     CDS             complement(<1..598)
                     /gene="ccsA"
                     /locus_tag="AC16VR_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HIR6010119.1"
                     /translation="MIVELGHLALVIALCLSGLLAVFGLGGLNLAGGRLLRANLSLVT
                     GQAVFCALAFGVLTQAFLADDFSVAYVANNSNTLLPWYYKFSAVWGAHEGSFLLWTLI
                     MSGWTLAVAVLGRTLPLEFHSRVLGVMGFLNLGFLSFLIFTSNPFERLVPLSPAEGSD
                     LNPVLQDFGLIVHPPMLYMGYVGFSVAFAFAVAALLSGR"
     gene            complement(595..1023)
                     /gene="ccmE"
                     /locus_tag="AC16VR_02840"
     CDS             complement(595..1023)
                     /gene="ccmE"
                     /locus_tag="AC16VR_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017849438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HIR6010120.1"
                     /translation="MNPKRRNRLIVVSFLLLGTMATAFFVLVALNENLNLFYPPDQIV
                     AGEAPLGTQIRAGGMVLEGSVARTGNELDVEFVLTDYQGAAFTVLYSGILPDLFREGQ
                     GILVRGELDPSGVFRAEEVLAKHDENYMPPELDGMHAGPP"
     gene            complement(1020..1211)
                     /gene="ccmD"
                     /locus_tag="AC16VR_02845"
     CDS             complement(1020..1211)
                     /gene="ccmD"
                     /locus_tag="AC16VR_02845"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HIR6010121.1"
                     /translation="MSFETFGDFLTMGGHGPYVWLSYGLTLAVVLVNVLAPITGRRRY
                     LREQADVERRRQAAEGVRT"
     gene            complement(1208..1951)
                     /locus_tag="AC16VR_02850"
     CDS             complement(1208..1951)
                     /locus_tag="AC16VR_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003114290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HIR6010122.1"
                     /translation="MKEFWHRLGSPRWFYGISGPWLKILAGLAALLLGTGCIWGLAFA
                     PPDYQQGNSFRIIYVHVPTAIVAQTAYLVMGCAGVVLLVWRMKLADMVLATVAPFGMW
                     MTVLALMTGAIWGKPTWGAWWVWDARTTSMLVLLFLYFGIYALRQAIPRQEAAGRACA
                     VLAVIGVVNIPIIKYSVDWWLTLHQPATFKLTAAPSMPVEMWLPLAVNVVGIYCLFGA
                     NLLSRLRLEILQREAGTQWVRDMVLEEAR"
     gene            complement(2076..2747)
                     /gene="ccmB"
                     /locus_tag="AC16VR_02855"
     CDS             complement(2076..2747)
                     /gene="ccmB"
                     /locus_tag="AC16VR_02855"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005740276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HIR6010123.1"
                     /translation="MAGLFRAQLRRELQTTARSVGVAVNPLVFLFLAMTLFALGAGGG
                     PDDLREHAPGIIWVLVLLASMLSLEALFRSDYDDGTLEQMLLLAEPLFVPLLAKITAQ
                     WCVSGLAMTLLAPLAALILSLPAASVPTLAATLLLGTPALSLLGAVGAALTVGLRRGG
                     VLLGLLILPLYVPVLIFGAGAVSADMAGTGAAGQLYWLAAITMLALTTAPFGVQAALK
                     ISVEQ"
     gene            complement(2771..3370)
                     /gene="ccmA"
                     /locus_tag="AC16VR_02860"
     CDS             complement(2771..3370)
                     /gene="ccmA"
                     /locus_tag="AC16VR_02860"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009621121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HIR6010124.1"
                     /translation="MEPDQVGAAAAPLLRVVDAGCRRGGRQLFTELSLELGAGECVEL
                     HGPNGSGKSTLLRCIAGLYPDFEGVVETAEFLYAGHRLGLNPLLSAEQNLAWYAALRG
                     TADAVLPALERVGMAGYERTACQHLSAGQQRRVALARLLVCRSPLWLLDEPLTALDGP
                     GQELAVTLVTAHMDGGGAVVAATHQPLGLERSRLLSLGR"
     gene            complement(3401..4789)
                     /gene="ppnN"
                     /locus_tag="AC16VR_02865"
     CDS             complement(3401..4789)
                     /gene="ppnN"
                     /locus_tag="AC16VR_02865"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008373414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HIR6010125.1"
                     /translation="MNTTTGQIRKVDVHVSPRGSLELLSQLEVNALASTAAESPLLDL
                     FRRCALAVLNTGSESDDAAAIFREYADFSIEVARRTRGVKLIIKNAPASAFVDGRMIE
                     GVRQHLFSVLRDIVYIGTDIADSDLFDLNSSEGITDAIFHILKNARVLRPDLPPRTVV
                     CWGGHSISRTEYEYTKEVGYHLGLRGLDICTGCGPGAMKGPMKGAAVGHAKQRQQDAR
                     YVGLTEPGIIAAEPPNPMVSDLVILPDIEKRLEAFLRIGHGVLVFPGGAGTVEEILYL
                     LGVLLDPANEGEEMPLIFTGPAESADYFFELDAFLRLSLGDEVARFYDIVIGDAEEVG
                     TRIGRRVRRVRRHRRQVGDAYYFNWLLNVPLAHQQPFKVTHENVAALRLSADLPAHEL
                     VVNLRRAFSAIVTGNVKDDGIRMIESHGPFELHSDRHLIGALEQLLRSFSENGRMKLI
                     GPYEPCYRVAAG"
     gene            5014..7110
                     /locus_tag="AC16VR_02870"
     CDS             5014..7110
                     /locus_tag="AC16VR_02870"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor family protein"
                     /protein_id="HIR6010126.1"
                     /translation="MSGSSMIRALLAVAALCSATGAATGNEPTDAAGPALEELVVSAT
                     RTNRTVREVPAAVSVVGREDIQLGRQQLGLDEALGRVPGLFSQNRYNFAQDLRLSIRG
                     FGARANFGIRGLKLIADGIPATTADGQSGVDDIDLGSLDRIEVIRGPSSSLYGASSGG
                     VINLMTEDGPERPFVEAGVTLGEYDQQRYQLKAGGQTGRLNYLASASHLAYDGYREHS
                     RVESTIFNSKFRYGIDSASNLTVIVNLVDSPTADDAGAITQAQVEDDPRQAQQRNLSS
                     NAGESIEQQKLGIIYDRALDDRNAITLRNYYVWRDFQAFLPIGTHIPFVADDGVVEFD
                     RFFWGGGIQWRFADSLFGRPFQMSAGVDADIQEDDRQRYLNEAGVKGALAFDQLEKAE
                     AYGAYLRGEIGLADSVRLALGIRWDTVDLSVDDSFLDNADQSADLDFDELSPSVGLVW
                     DLGESWSAYANYATAFETPTFTELAVPARDLDVSLGGFANVSAQEAQSFEAGLRGGLL
                     DGALYVEAAAFTMEVDDEITSVSNIGNRSFFANADTDRRGIETFANLAFEGGLELSLA
                     YTYSDFEFDRFEDNEDFEGNALPGLPEHQLFAEIKYRHGSGAYAVADVLHVSKLYVNN
                     ANSGTSEASTVANLRVGASLALGQWQLDPFLGVNNLFDENYISNVRINGFGGRLFEPA
                     PKRNIYGGVAVRYVLR"
     gene            complement(7129..7359)
                     /gene="nrtS"
                     /locus_tag="AC16VR_02875"
     CDS             complement(7129..7359)
                     /gene="nrtS"
                     /locus_tag="AC16VR_02875"
                     /inference="COORDINATES: protein motif:HMM:NF038050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NrtS"
                     /protein_id="HIR6010127.1"
                     /translation="MSTQHGFLRLAARPDVVARAARTALLVGVILIAINHGDALLSGD
                     VGPVQAGKMALTVLVPYCVSTYASVRALQSAG"
     gene            complement(7356..7853)
                     /locus_tag="AC16VR_02880"
     CDS             complement(7356..7853)
                     /locus_tag="AC16VR_02880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010128.1"
                     /translation="MLRRNTVWLVLVATGLLASTAYPPEAAARVTLSNTVARYEIAQG
                     LDGQVERRLVAADEVLPGDELLYTITFANEGREEVAAGSIVITNPIPEVAEYVEGSAA
                     GEDTFITFSVDGETFAEPSGLMVQRGDGQRLAEPSEYASIRWAYQQALEPGESGAVSF
                     ALRLR"
     gene            complement(7946..8191)
                     /locus_tag="AC16VR_02885"
     CDS             complement(7946..8191)
                     /locus_tag="AC16VR_02885"
                     /inference="COORDINATES: protein motif:HMM:NF018500.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HIR6010129.1"
                     /translation="MGCGSGHENDGAAIKVPLSELSEEALAGVVESFVLREGTDYGHR
                     EFTLAEKRALVRKQLESGDAVIYFDPLTRSIDIKPAR"
     gene            8279..8986
                     /locus_tag="AC16VR_02890"
     CDS             8279..8986
                     /locus_tag="AC16VR_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008093405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6010130.1"
                     /translation="MAEPDHILVVEDEPITREQLVSYFEEEGFRVTSTGSGDEVHELV
                     KNSDIILVLLDIKLPGKDGLTLTREIRAQSDVGIILVTSKQEQIDRILGLESGADDYV
                     TKPFDPRELLSRARNLIRRVQLQQRQRRKNHIRNFEGWQLDLNKRELTSPEGEKTTLS
                     AGEYQLLLAFMEQAGEVMNRDQLMNRIRNREWYPDDRYIDVLVGQLRKKLGERAANAK
                     IIATIHGTGYLFTPEVA"
     gene            9053..15367
                     /locus_tag="AC16VR_02895"
     CDS             9053..15367
                     /locus_tag="AC16VR_02895"
                     /inference="COORDINATES: protein motif:HMM:NF019334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR6010131.1"
                     /translation="MSSVDEKIYESASTVVRRTTWQDQPVVAKRLKPEGLPSNVLSQF
                     HHEFHLNQSLTSPNVCRALAMDESGLEIYFEDTQGASLRSLIDTGELSLDERIAIAVE
                     LSRALQSVHDEGVIHRDVNPGNVLVSEDPMRVHLIDFGLATLAAREYPEVEPLGQVTG
                     TLPYLSPEQTGRVNRVVDYRTDLYSLGATLYELLAGNPPFTNTDPLELIHAHIASTPR
                     PLTAIGEGIPRWLSEIVQKLLAKQPEDRYQSAAAVRDDLIRGQTQANVVPFRLGQTDS
                     PGLLAMPKRLYGRTAQIGIIQEYLERAARGEVLFLQITGAQGRGKSSLCAELARQAAG
                     TKAMVARTNALAFDFRDTDAVWIELTRQLLRQALADAKAGDLLARIAALNSPNVRSLT
                     ELIPELRDLVPAGSEGAGLPGVGIQEVLKALQPQPLCLIVEEVDNVPSESLETLLSMA
                     LDSRHLLMTVTRANADSDAFRTPRLATKAATLELPAFARADVRSLLSDMLSTSEVRVR
                     ELASELHAKTDGLPADVLQLIEELHHLGHIFRDANAAEWEWDMAAIRAHYFSNNSADR
                     IRRLISELSEETTHALQVGAAVGEQFSSELAATLLDVEQAELAGRLRPALTKGLLTTA
                     TGSKEAPGDQHQLLYRFAHPRVRSLVYAEMAQEAKSTIHFAIADECKRGHRPAGRTLL
                     QIAHHLNAAADPIGLDKDKRLEIAHYNLLAAKESIGQGAFQPAYKFCRSGLSLLSELP
                     DEPIFIELCQSAAEAAFYCSDFEQLDRVIASIPHNTSALDEIRVRAAVVQNHLGAARE
                     QAFDALESLGYVRPATGINPIGQAFRRLGRKRAPDAAPKPAPVLEDIRLQHTFRLLGY
                     LLHAGYHLGSEGLDALTDDMLSEAARHGYSAEIAFAFAARAVSAVADHRIGIAQRYAG
                     EARALAGQFSGDAFSIRAETLLNGLVDPWSGGLDDTLGGLSRNIDASMACQDYEFAAT
                     ATAFYATNGLLRGLDLASLRRELNEQISRLSGLQHVTGVNISLFVQQIIGSLLGHAEA
                     DGESDHFRELSISNSEDAVAHGYVYVLRLYYAVLFDDFRGAANILELAEQFGPALAGS
                     PLLIVWKFADALVNLRSGNTAGLRRARASLRELKQWHQDGADHAEPKVILLQAELNWQ
                     RGATTQAIECYEVAADKARRLGLANDEALAYELAARACESNGRADFAKLFARNAHQAY
                     LRWGANAKASHLEREFPSMLDDALHPATVIGDLTDLTVRDFSSMSIRSSLDGSEINAN
                     VLDTTTVLRAAQTISSEIVLDRVLTKLLRLALEHAGAQKACMLLAHDGRLFVEAIASV
                     DGGPTRRESPASPLEASDDVPESIVHFVARTKQALVLGDATAEDVFTQDPYIVRKQPL
                     SVLCVPIVHRNEITGILYVEHRWLTGVFTAQRVEVLALLASQAAISIENARLYADLHD
                     TQEQYRALYDNAIEGLFRIDPDGALLSANPTLARILGFDDVKQLLAEYRDLIDRVFLS
                     REDASHFLTNLEEHGLLNVFEAQGVTRNGRTFWMALTARLSHDPEQGAYIDGSLVDIS
                     ERMKREQADKHRQIAEAATQAKSEFLANMSHEIRTPMNAIIGFSKLALETGLDPKQHE
                     YLSSIRAASENLLSLVNDVLDFSKIEAGKLTLEHRPFKLADTLKEVERLFRTEVRKSN
                     LELNFDDNTAAHAEFPGDGVIIGDPLRLQQVLVNLVSNALKFTEEGSISVTAQVCPCE
                     AGELRVEFAVRDTGIGIKPEHQARLFESFEQAETSTTRRYGGTGLGLTICKRLVSLMG
                     GEMSVASVPDQGSCFTFTAVFGLRDAQDLMPADVRQRRRNLRALNRREFLVAEDNPIN
                     QQLSLEFLQRTGARVDIAQTGREAVERATSKAYDAILMDIHMPELDGLQACRILREQQ
                     ITTPIIAVSADAIEASQQAALEAGCNAYVSKPIDFDLLLAELSRVLPEQPADAASVDA
                     PASAPAEHEASTTPGELPEIQLPGIDLDTAIRGHNGNVRLMLKLMGDFGRYYGDAGAK
                     MRAHVTDNHLEEAERLAHNLHGVAGSFGADRLKQASKTLELALAKGDESNLLGLVQSF
                     EVAMNEVLESAETLANHPFTEVASRSRNEG"
     gene            complement(15330..16181)
                     /gene="ttcA"
                     /locus_tag="AC16VR_02900"
     CDS             complement(15330..16181)
                     /gene="ttcA"
                     /locus_tag="AC16VR_02900"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HIR6010132.1"
                     /translation="MRKHGYDMNKLHRKLRRKVGQAIADYGMIEEGDRVMVCLSGGKD
                     SYTLLDILLNLQRSAPVAFEIVAVNLDQKQPGYPADVLPGYLEGLDVSYHILEQDTYS
                     IVKAVTPAGKTTCPVCSRLRRGILYNFAASIGASKIALGHHLDDVVETLFLNMFHGAR
                     LKAMPPKLKSDDGRNVVIRPLYYCRERDIARWAEHREYPIIPCNLCGSQENLQRQVIK
                     SMLARWDAEQPGRVENIARSLSAVVPSHLGDRGLFDFAGLSSDDGVEKLQLLDLTPRC
                     VNAMPPR"
     gene            complement(16295..18787)
                     /locus_tag="AC16VR_02905"
     CDS             complement(16295..18787)
                     /locus_tag="AC16VR_02905"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6010133.1"
                     /translation="MSIKLVLRLAWRDWRGGELGLLLVALMLAVGTVTAVSLFVDRLH
                     QALIDESADFLAADRRISGSRPIPDEFRLAASAKGVEHADTMSFPSMVFVGDDNSQLV
                     SVKAVGAGYPLRGVLKIADEPFAPGVPTSDIPAPGEVWLDARLFPALGIRPGDSIEVG
                     VAPLKVAKVLTSEPDRGGSMYDLGPRLLMRLDDVPATQVVQPGSRIAYRLLLRGAESD
                     LESLKAELPLAPNYRWQGIRESSPSIGSALARAESFLLLGGLLGVLLAGVAVALSAHR
                     YALRHYDHVGILKTLGATPNQILSGYLMLLLAVGSAAVVLGLTGGAALHLGIVALLQS
                     LIPVDLPMPSARPFLLGAATGLICAVAFALPPLLHLKNVSPMRVIRRDLGAAPPSQYL
                     SYGAAVAGSVGLLIWYSGSASLTAWTILGTVVVLLVFGALAMLLLRGGRAVGMQAGSG
                     WRLALAGLQRRRRENVAQILIFGLAIMLLLILVLLRSSLLDEWQGQVPEDAPNHFVLN
                     IVPHEVDELATMLDDFTGSHGGLFPMIRGRIVAANGVDADAWERDHQPVDAPGPRLQS
                     ERNLTWAADLPDDNQVVKGRWWSPDEARALVSLEDEYAQSLGLDVGDRLRFDIAGRFV
                     EVEVASLRRLDWESMRPNFFIIFAPQVLSDFPATYMTSFHLERDEKPFLNRFLARFPT
                     VTVIEVDAIIAQVQSIILRVTQAVELVLALVVASGCLVLVASIQASRDGRMSELALVR
                     TLGGSRRLIAGSLVAEFAALGLFAGTVAVVGAEATVAILQSQVFELPVRLHPWLWLLG
                     PVIGSVLILIVGTLGTRGLISTPPVMVLRGLD"
     gene            complement(18784..19458)
                     /locus_tag="AC16VR_02910"
     CDS             complement(18784..19458)
                     /locus_tag="AC16VR_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002553014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6010134.1"
                     /translation="MTNQPVIRTEGVSKSVPTSEGELSILESIDLEINAGETVAIVGA
                     SGSGKTTLLGILAGLDLPTSGRVYLVGEELTALDEEQRARVRGEQVGFVFQTFQLLRS
                     LTALENVMLPGELRGDGDAQPEALRVLDKVGLGARTGHYPTQLSGGEQQRVAIARAFA
                     SRPNVLFADEPTGNLDTATGQRIIDLLFELNAEYATTLVLVTHDDRLASRCERVVEIV
                     AGRITS"
     gene            19478..20068
                     /locus_tag="AC16VR_02915"
     CDS             19478..20068
                     /locus_tag="AC16VR_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HIR6010135.1"
                     /translation="MIALIAGVSPASARSKILVMGDSISAAYGIQRDAGWVALLDRRL
                     SETASPWQVVNASISGETTGGGLARLPKALEAHDPEIVIIELGGNDGLRGYPVSRIRE
                     NLDKLVELAVTGGRRVLLLGMRIPPNYGPRYTRAFEDMYRDVAEAHGVRFVPFILEAV
                     ALNPGLMQDDGIHPTAEAQQKLLDSVWPELQQVLDG"
     gene            complement(20065..>20892)
                     /gene="trhO"
                     /locus_tag="AC16VR_02920"
                     /gene_synonym="yceA"
     CDS             complement(20065..>20892)
                     /gene="trhO"
                     /locus_tag="AC16VR_02920"
                     /gene_synonym="yceA"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF001133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent tRNA uridine(34) hydroxylase
                     TrhO"
                     /protein_id="HIR6010136.1"
                     /translation="LAGEGINGTVTGNAVALQKFLGALERLPEFSGLAVKYSSAAAGN
                     PVFHRLKVRVKPEIVSFGVSGLDPGGRTGTHVGAEEWNELLDDPEVTVVDTRNDYEVA
                     IGSFPGAVNPATQSFRQFADFVADHLDPDRDTRVAMFCTGGIRCEKASAYLLERGFAE
                     VYQLDGGILNYLETVKGEANRWEGECFVFDQRVSVDRHLREGSYEQCFACRRPLSTDD
                     LQSPDYRPGISCPHCAQEQPEARRAALAERARQVALAARRGEAHIGVRQGSQGRKNG"
BASE COUNT         3964 a         7060 c         6618 g         3250 t
ORIGIN      
        1 gccgaccgga cagcagcgcg gccacggcaa aggcgaaggc cacggagaaa cccacgtagc
       61 ccatgtagag catcggcggg tgaacgatta gcccgaagtc ctggaggacc ggattcagat
      121 cagagccttc cgcgggagag agcggaacga gccgctcgaa ggggttgctc gtgaagatga
      181 ggaacgaaag aaagcccaga ttgaggaagc ccatcacccc cagcacccgg ctgtgaaact
      241 ccagcggcag ggtccgtccc agcacggcca cggcaagcgt ccagccggac atgatcagcg
      301 tccagagaag aaaagaccct tcgtgagcac cccagacggc gctgaacttg tagtaccagg
      361 gcagcagcgt gttggagttg ttcgccacgt aggcgacgga aaagtcgtcg gcaagaaaag
      421 cttgggtgag cacgccgaag gccaacgcgc agaacactgc ctggccggtg accagcgaca
      481 ggttcgcccg gagcagcctg ccccccgcca gattcagacc gccgaggccg aacacggcaa
      541 gcagcccgct caggcagagc gcgatgacca gtgccagatg tcccagctct acgatcacgg
      601 cgggccggcg tgcatgccgt ccagctccgg aggcatgtag ttctcgtcgt gcttcgccaa
      661 cacctcctcg gcccggaaga cgccgctcgg gtcgagctcg ccgcggacga ggattccctg
      721 accttcgcgg aacagatcgg gcaggatgcc gctgtagagc accgtgaagg ctgcgccctg
      781 gtaatcggtg agcacgaact ccacgtcgag ctcattgccc gtgcgcgcca cgctgccctc
      841 gagaaccatg ccacctgccc ggatctgcgt gcccagcggc gcttcgccgg ccacgatctg
      901 atccggcggg tagaagagat tcagattttc gttgagcgcc accagcacga agaacgccgt
      961 cgccatggtc ccgaggagca ggaaggacac aacgatcaac cggttacgcc gtttcggatt
     1021 cacgtccgca caccctccgc cgcctgcctt cgtctttcca cgtccgcctg ctcacgcagg
     1081 tagcgccggc ggccggtgat cggcgccaac acgttgacca gcaccacggc cagcgtcagc
     1141 ccgtagctca accacacgta cgggccgtgc ccacccatcg tcagaaagtc accgaacgtt
     1201 tcgaagctca ccgggcctcc tcgagcacca tgtcgcgcac ccactgcgtg cccgcttccc
     1261 gctgcaggat ctccagtcga agacgactca acaggttggc accgaacagg cagtagatgc
     1321 cgacgacgtt caccgccaga ggcagccaca tctccaccgg catgcttggc gccgcggtga
     1381 gcttgaaggt agcaggctga tgcagggtca accaccagtc gacggagtac ttgatgatgg
     1441 ggatgttcac caccccgatg acggccagca cggcgcaggc gcgcccggcg gcctcctgcc
     1501 ggggtatggc ctgacgcagc gcgtagatcc cgaagtacaa aaagagcagc accagcatgg
     1561 aagtcgtgcg cgcatcccac acccaccagg cgccccaagt gggcttgccc cagatcgcac
     1621 ccgtcatcag cgccagcacc gtcatccaca tcccgaacgg cgcaaccgtt gccagcacca
     1681 tgtctgcaag cttcatgcgc caaaccagca ggaccacgcc ggcgcacccc atcacgagat
     1741 aagcggtctg ggccacgatg gcggtgggca cgtggacgta aatgatccgg aaactgttgc
     1801 cctgctggta gtcgggcggg gcgaaggcga gcccccagat acatcccgtg cccagcagca
     1861 gcgccgccag ccctgcgagg atcttcagcc agggaccgct gatgccgtag aaccagcgcg
     1921 gggatcccag ccggtgccag aattccttca gcgtcgccaa gggccgccct cacgtcgccg
     1981 tcgtaacgtt gtcgtttgcc gagcaatcat atgcatccgc gatatgcatc cgcgtagttt
     2041 gacgcccagg aggggccggg gttcgccttt catcgtcact gctccacgct gatcttgagc
     2101 gctgcctgaa cgccgaaggg cgccgtggtc agcgccagca tcgtgatggc ggcaagccag
     2161 tagagctgac ctgccgcccc cgtccccgcc atgtccgcgc tgaccgcgcc ggcgccaaat
     2221 attaaaaccg gcacatacaa aggcaaaatc agaagcccca gaagcacccc gcccctgcgc
     2281 agcccgacgg tgagcgcagc gcccacggcg cccaaaaggc tcagggccgg cgtgcccagc
     2341 agcagggtcg ccgcaagcgt cggcacgctg gcggccggca gagacaggat caacgccgcc
     2401 agcggggcga gcagggtcat ggccaggccg ctgacgcacc actgcgcggt aatcttcgcc
     2461 agcaggggca cgaagagcgg ctcggccagc aacagcatct gctccagcgt cccgtcatcg
     2521 taatcgctgc gaaacaacgc ttccagcgac aacatgctgg cgagcagcac cagcacccag
     2581 atgatgcccg gggcgtgctc gcgcaggtcg tcgggcccgc ccccggcgcc aagggcaaag
     2641 agcgtcatcg cgaggaaaag gaacacgagc gggttcaccg ccacacccac cgatcgtgcc
     2701 gtcgtctgca gctcgcgccg cagctgagcc cggaacaggc cggccaccgt gttctccttc
     2761 gcggagccgc tcatcgaccc aggctcagca ggcgcgaacg ctcgagcccg aggggctgat
     2821 gggtcgctgc caccacggcc ccgccgccgt ccatatgcgc ggtgacgagc gtcacggcga
     2881 gctcctgccc cgggccgtcc agcgcggtca gcggctcgtc gagcaaccac agcggcgagc
     2941 ggcagacgag cagccgggcc agcgccaccc gacgctgctg gccggcggac aggtgctggc
     3001 aggcggtacg ctcgtaaccg gccatgccca cacgctccag cgccggcagc acggcgtctg
     3061 cggttccccg caaagcggcg taccaagcca ggttctgctc agccgacagc agcgggttca
     3121 gacccagccg atgtcccgca tagagaaact cggcagtctc gacgacgcct tcaaagtctg
     3181 gatacaggcc ggcaatgcag cgcagaagcg tcgactttcc gctgccgttg gggccatgca
     3241 gctccacgca ctcaccagcc ccgagttcca gggagagctc cgtgaacagc tgccgaccgc
     3301 ctcggcggca gccggcgtcg acgacccgga gcaacggcgc agctgctgct ccaacctggt
     3361 ctggttccaa aggcgctggc aaggcgatga ccgtccagac ctacccggcg gcaacccggt
     3421 agcagggctc gtaggggccg atgagcttca tgcggccgtt ctcggagaag cttcgcagca
     3481 gctgctcgag cgcgccgatg aggtgccggt cgctgtgcag ctcgaacggc ccgtggcttt
     3541 cgatcatgcg aatgccgtcg tccttcacgt tacccgtgac gatggccgag aaagcgcgcc
     3601 gcagattgac caccagctca tgggccggca ggtcggcgct caggcgcagg gcggcgacgt
     3661 tttcatgggt gaccttgaac ggttgctggt gagcgagggg aacgttgagc aaccagttga
     3721 aatagtaggc gtcacccacc tggcgccggt gccggcgcac ccgacggacc cggcggccga
     3781 tccgggtgcc cacctcctcc gcgtcgccta tcacgatgtc gtagaagcgg gccacctcgt
     3841 cgcccagcga aagcctgagg aaggcatcga gctcaaaaaa gtagtccgcg ctctccgcgg
     3901 gaccggtgaa gatcaacggc atctcctcgc cctcgttggc gggatcgagc agcacgccga
     3961 gcaggtagag gatttcctcc acggtgcccg cgccgcccgg aaacaccagc acgccgtgac
     4021 cgatgcggag gaaggcctct agccgtttct cgatgtcggg cagtattacc agatcgctca
     4081 ccatggggtt cggcggctcg gcggcaatga ttcccggctc cgtgaggcct acgtaacgag
     4141 cgtcctgctg gcgctgcttg gcgtggccga ccgccgcgcc cttcatcggt cccttcatgg
     4201 cccccggacc gcagcccgtg cagatgtcta ggccccgcag accgaggtgg tagccgactt
     4261 ccttggtgta ctcgtactcg gtccgggaaa tcgagtggcc gccccagcag accacggtgc
     4321 gcggcggcag gtcgggacgt agaacccggg cattcttgag gatatggaaa atcgcatccg
     4381 tgatcccctc ggagctgttc agatcgaaaa gatcgctgtc cgctatgtcc gtaccgatgt
     4441 agacgatgtc ccgcagcacc gagaagaggt gctgacgcac cccctcgatc atccggccat
     4501 cgacgaacgc ggaagccggc gcgttcttga tgatcagctt gacgccccgc gtgcgtcggg
     4561 cgacctcgat gctgaagtcg gcgtactctc tgaagatggc ggcagcatcg tcggattcgc
     4621 tgcccgtgtt caggacggcc agcgcgcagc gccggaacag atcgagcagc ggcgactcgg
     4681 ccgcggtgct ggccagcgcg ttgacctcga gctgcgagag cagctccagg gatcccctcg
     4741 ggctgacgtg gacgtcgacc ttgcgaatct gaccggttgt ggtgttcatg ccgggaagtc
     4801 taccgattcg cgctgccgac acccaccgct cgatgcgccg cgccggccga gccggcccgc
     4861 atcgggcggt aacccaaagc ggattctgat agtgtacccg cggggcgcgc gtccggcctg
     4921 acgcgcttgc gggcacggtc gattcgaagg cgatcggccc gggaaagccg tgtgccccag
     4981 cccaagagaa tttcgatcgg aagaggagga tcgatgtccg ggtccagcat gatccgtgca
     5041 ctgctcgccg tggccgcgct ttgcagcgcg accggcgccg ccactggcaa cgagccgacg
     5101 gacgccgctg gccccgcgct ggaggagctc gtcgtcagcg cgacacgcac gaaccgcacg
     5161 gttcgcgagg tcccggctgc cgtcagcgtg gttggccggg aggacatcca gctcggacgg
     5221 cagcagctcg gcctcgacga ggccctcggt cgggttccgg gacttttttc ccagaaccgc
     5281 tacaacttcg cccaggacct gcgtctgtcc atccgcggat tcggcgccag ggcgaatttc
     5341 ggcatccgcg gcctgaagct gattgccgac ggcatccctg cgaccacggc cgacggccag
     5401 agtggcgtcg acgacatcga cctggggtcg ctcgaccgca tcgaggtgat ccgcggaccg
     5461 tcgtcctcgc tgtacggcgc gtcctccggc ggcgtcatca acctgatgac ggaggacggc
     5521 ccggagcgtc cgttcgtcga agccggcgtc accctcggcg agtacgacca gcagcgatac
     5581 cagctcaaag cgggcggtca gaccggacgg ctgaactacc tggccagcgc ctcgcatctg
     5641 gcttacgacg ggtatcgcga gcactcgagg gtggagtcga caatcttcaa ctccaaattc
     5701 cggtacggga tcgactcggc gtcgaacctc acggtgatcg tcaacctggt cgactcgccg
     5761 acggcagacg acgccggcgc cattacccaa gcccaggtcg aggacgatcc ccggcaggcg
     5821 cagcagagga acttgagctc caacgccggt gaatccatcg aacagcagaa gctcggcatc
     5881 atctatgacc gcgctctcga cgaccgcaac gcgatcacgc tgcgcaacta ctacgtctgg
     5941 cgcgatttcc aggcgtttct gcccatcggc acccacatcc ccttcgtcgc cgacgacggc
     6001 gtggtggagt tcgaccgctt cttctggggc ggcggaatcc agtggcgctt cgccgattcg
     6061 ctgttcggcc ggccgttcca gatgagcgct ggggtggacg cggacatcca ggaggatgat
     6121 cgccagcgct atctcaacga ggccggcgta aaaggggccc tggccttcga ccagctggag
     6181 aaggccgaag cctacggcgc ctacctgcgg ggagaaatcg gactggccga ttcggtccgc
     6241 cttgccctgg ggatacgctg ggacacggtc gacctgtcgg tggacgacag tttcttggac
     6301 aacgcagacc agtccgcgga cctggacttt gacgagttga gcccctcggt cggcctggtc
     6361 tgggatctgg gcgagagctg gagcgcttac gcgaactacg ccaccgcctt cgagacaccc
     6421 accttcaccg agcttgccgt tcccgcaaga gacctcgacg tgagccttgg cggctttgcg
     6481 aacgtctcgg cccaggaggc gcagagcttc gaggccggtt tgcgcggcgg cctgctcgac
     6541 ggcgccctgt acgtcgaggc agccgctttc accatggaag tggacgacga gatcaccagc
     6601 gtttccaaca tcggcaatcg gtccttcttc gccaacgcgg acaccgaccg gcgcgggatc
     6661 gagaccttcg ccaacctcgc cttcgagggc gggctcgagc tgagcctggc gtacacgtac
     6721 tcggacttcg agttcgatcg gttcgaggac aacgaggact tcgaaggcaa cgcccttccc
     6781 ggactgcccg agcatcagct ctttgctgag atcaagtacc ggcatggctc gggcgcctac
     6841 gccgtcgccg acgttctgca cgtcagcaag ctgtacgtga acaacgccaa ttccggcacc
     6901 agcgaagcgt ccaccgtcgc caatcttcga gtcggcgcgt ccttggcgct gggccagtgg
     6961 cagctcgatc cgtttctcgg cgtcaacaac ctcttcgacg agaactacat ttccaacgtg
     7021 cgcatcaacg ggttcggcgg caggctgttc gagccagctc cgaaacggaa catctacggc
     7081 ggcgtggccg ttcgctacgt gctgcgctga tggttcgggg agcgccgatt accccgcgct
     7141 ctgcagcgct ctgacgctgg cgtaggtgga tacgcagtaa ggcaccagca cggtcagcgc
     7201 catcttgccg gcctgcaccg ggccgacgtc tccggacaac agcgcgtcgc cgtggttgat
     7261 ggcgatgaga atgaccccga ccagaagcgc cgtccgggcg gctcgcgcga ccacgtccgg
     7321 gcgggcggcc agacgcagaa acccgtgttg cgtgctcatc gcagacgaag ggcgaacgat
     7381 accgcgccag actctccggg ctcgagagcc tgctggtagg cccagcgaat cgaggcatat
     7441 tcggagggct cggcgagccg ctgcccgtct ccgcgttgga ccatcaggcc cgacggctcg
     7501 gcgaaggttt ctccatcgac cgagaaggta atgaaggtgt cctcacccgc cgcgctgccc
     7561 tccacatact cggccacctc gggaatcggg ttggtgatga cgatgcttcc tgcggccacc
     7621 tcctctcgcc cctcgttggc aaacgtgatg gtgtagagca gctcgtcgcc gggaagcacc
     7681 tcgtccgcgg ccacgagccg tcgctcgacc tgcccgtcca ggccctgcgc gatctcgtac
     7741 ctggccaccg tgttggagag cgttacgcgc gctgctgctt caggggggta tgccgtcgag
     7801 gcgaggaggc cggtggcaac gagcaccaac cagaccgtgt ttcgcctcag cacattcaag
     7861 atcacacttc ctccagagcc ttccggcacg cttggaccct ccgttccggg aatcgatgtt
     7921 ctcacaccca gcgcatcgca gaccattacc gagcaggttt gatgtcaatg ctgcgggtga
     7981 gagggtcgaa gtagatgacc gcatcaccac tttcgagctg cttgcggacc agcgcgcgct
     8041 tctccgcgag cgtgaattcc cgatgtccgt agtcggtgcc ctcgcgcagc acgaacgact
     8101 cgacgacgcc cgcaagggct tcctccgaga gctccgacag gggaactttg atcgccgccc
     8161 catcattttc gtgacctgat ccgcatccca tgtcgctcgc cttgaatttc tgaacctaag
     8221 gtgcgttact tccccttact gaaatcgtca taccggcgtt ccaacaggac ggggcaccat
     8281 ggccgaacca gatcacatac tcgtcgtcga agacgagccg attacccgcg agcaactcgt
     8341 atcctacttc gaggaagaag gtttccgcgt cacttcgacc ggatccggcg acgaggtgca
     8401 cgagctggta aagaacagcg acattatact cgttctcctg gacatcaagc tgcccggcaa
     8461 ggacggtctg acgttgaccc gcgagatccg cgctcaatcc gacgtcggca tcatcctggt
     8521 aacaagcaaa caggaacaga tcgaccgcat tctcggtctg gagagcggcg ccgacgacta
     8581 cgtgacgaag cccttcgacc cgagggagct gctcagccgc gcccgcaacc tgattcggcg
     8641 cgtccagctt cagcagcgcc agcgccgcaa gaaccacatc cgcaacttcg agggctggca
     8701 gctagatctg aacaaacgcg agctgacttc tccggaaggc gagaagacga cgctgtcggc
     8761 aggcgagtac cagctcctgc tggcattcat ggagcaggcc ggcgaggtca tgaaccgcga
     8821 tcagctgatg aaccgcatcc gtaaccgcga gtggtatcct gacgaccgat acatcgacgt
     8881 gctggtcggc cagctccgca aaaagctcgg cgagcgggcg gccaacgcca agatcatcgc
     8941 caccatccac gggacgggat acctgttcac acccgaagtg gcctgaattc gttcagcaaa
     9001 cgggatgtga cgatcctgaa cagcgtgtgg gggcacagac gggggaaaag gcgtgagcag
     9061 cgtggatgaa aagatctacg aaagcgcgtc gacagtcgtc cgacggacca cctggcagga
     9121 tcagcccgtc gttgcgaaac ggctgaagcc cgagggcctg ccctcgaacg tgctcagcca
     9181 gtttcatcac gaattccacc tgaatcagtc gctgaccagc cccaacgtct gtcgcgcgct
     9241 cgccatggac gaaagcggcc tcgagatcta cttcgaggac acccagggcg cctctctgcg
     9301 cagcctcatc gacaccggcg agctttccct ggacgaacgc atcgcgatcg ccgtggagct
     9361 cagccgcgcg ctgcagtccg ttcacgacga gggcgtgatc cacagggacg tcaaccccgg
     9421 caacgtgctc gtctccgaag accctatgcg cgtgcacctc atcgacttcg gcttggctac
     9481 gctagcagcc cgggaatatc ccgaggtgga accccttggc caggtgaccg ggaccctgcc
     9541 ctacctttca cccgagcaga ccggccgcgt gaaccgagtg gtggactacc gcacggacct
     9601 gtactcgctg ggcgcgacgc tctacgagct tctggccggc aaccccccgt tcaccaacac
     9661 ggacccgctg gagctcatcc acgcgcacat cgcgagcacg ccgcggcccc taaccgccat
     9721 cggcgaggga attccgcgct ggctgtcgga aatcgtccag aaactgctcg ccaagcaacc
     9781 ggaagaccgc taccagagcg ccgcagccgt ccgcgacgat ctgatcaggg ggcagaccca
     9841 ggccaacgtg gtaccgttcc ggctcggaca gaccgactcg cccgggctgc tggccatgcc
     9901 caaacgcctg tacggtcgca ccgcccagat cggcatcatt caggaatatc tggagcgcgc
     9961 cgcacgaggg gaagttctct tcctgcagat caccggggcg caaggcagag gaaagtcctc
    10021 cctatgcgcg gagctcgcca ggcaggcagc cggcacaaaa gccatggtcg cccggaccaa
    10081 tgcgcttgcc ttcgactttc gcgacaccga cgcggtctgg atcgagctga cccgccagct
    10141 cctgcgacag gcgctggccg acgctaaagc cggtgacctt ctggcgcgca tcgcggcgct
    10201 gaactcgccg aacgttcgca gcctgacgga gctcatcccg gagctgagag atctggtgcc
    10261 tgcgggcagt gagggcgcgg ggctgcccgg ggtgggcatc caggaagtcc tgaaagcgct
    10321 gcagccccaa ccgctttgcc tgatcgtgga ggaagtcgac aacgtccctt cggaaagcct
    10381 ggaaaccctg ctgagcatgg cgctcgactc gcggcacttg ctgatgacgg ttacccgggc
    10441 gaacgccgat tcggacgcgt tcaggacgcc ccgactggcg accaaggcgg ccacgctgga
    10501 gctgccggca tttgcgcggg ccgacgtgag aagccttctg tcggacatgc tctcgaccag
    10561 cgaggttcga gttcgcgagc tcgcaagcga gctgcacgcc aagaccgacg gcctgcccgc
    10621 cgacgtcctt cagctgatcg aggagctgca tcacctcggc cacatcttcc gtgacgccaa
    10681 tgcggccgag tgggagtggg acatggcggc catccgcgcc cactacttca gcaacaactc
    10741 cgcagaccga atacgccggc tgatcagcga gctttccgag gagacgacgc acgcgctgca
    10801 ggtcggtgcg gccgtgggag agcaattctc gagcgagctg gccgccacct tgctcgacgt
    10861 cgagcaggcc gagctcgccg ggcggctgcg gccggcgctg accaaaggcc tgctcaccac
    10921 cgcgacgggc agcaaggaag cgccgggaga tcagcaccag ctgctctacc ggttcgccca
    10981 tccaagggtg aggtcgctgg tgtacgcgga gatggcccag gaagccaagt cgacgattca
    11041 cttcgccatc gccgatgagt gcaagcgcgg ccacaggccg gcgggcagga cgctcctgca
    11101 gatcgcccat cacctcaacg ccgccgcgga tcccattggg ctcgacaagg acaaacgcct
    11161 ggaaatcgcg cactacaacc ttctcgccgc aaaggagagt atcggccagg gcgccttcca
    11221 gcccgcctac aagttctgcc gcagcgggct gtcgctcttg agcgagctgc ccgacgagcc
    11281 catcttcatc gagctgtgcc agagcgctgc cgaggccgcc ttctactgca gcgacttcga
    11341 gcagctcgac cgggtcatcg ccagcattcc gcacaatacc agcgccctgg acgagatcag
    11401 ggttcgcgcg gcggtggtcc agaatcacct gggcgcagcc cgcgagcagg cgttcgacgc
    11461 cctcgagagc ctggggtacg tgcgacccgc aaccggcatc aaccccatcg gccaggcttt
    11521 ccgcaggctc ggcaggaagc gcgcgccgga cgccgcgccc aaaccggccc cggtgctcga
    11581 ggacattcgc ctgcagcaca ccttccggtt gctcggctac ctgttgcacg ccggatacca
    11641 cctgggcagc gagggcctgg acgcccttac agacgacatg ctcagcgagg cggcacggca
    11701 cggctacagc gccgagatcg cctttgcgtt cgccgcgcgt gccgtcagcg ccgtcgccga
    11761 ccaccggatc gggatcgcgc agcgctacgc cggggaggcg cgagcccttg ccggccagtt
    11821 ctcaggggac gccttctcca tccgcgcgga aaccctgctg aacggcctgg tggacccatg
    11881 gtcggggggg ctggacgaca cgcttggcgg gctttcgcgc aacatcgacg ccagcatggc
    11941 ctgccaggac tacgagtttg ctgccacggc cacggccttc tacgcaacca acggcctgct
    12001 tcgcgggctc gatctggcgt ccctacggcg agagctgaac gagcagattt ccagactttc
    12061 cggtcttcag cacgtcacgg gcgtcaacat ctcgctgttc gttcagcaga tcatcggcag
    12121 cctgctgggt catgcggaag ccgacggcga gtcggaccac ttcagggagc tgagcatttc
    12181 caacagcgag gacgcggtcg cccacggcta cgtgtacgtg ctgcgcctct actacgccgt
    12241 gctcttcgac gatttcaggg gggcggccaa cattctcgag ctcgccgagc agttcggccc
    12301 cgcgctggcg gggtcgcctc tgctcatcgt ctggaaattc gccgacgctc tggtgaacct
    12361 gcgctcgggc aacacggctg gcctacgaag ggcacgcgcg tcgctgcgtg agctgaaaca
    12421 gtggcaccag gacggcgccg accacgcgga gccgaaggtc atcctgctgc aggccgagct
    12481 gaactggcag cgcggcgcca ccacccaggc catcgagtgc tacgaggtcg ccgccgacaa
    12541 agcgcgtcgg ctcggccttg ccaacgacga ggcgctggcc tacgagctgg cggccagagc
    12601 ctgcgagtcc aacggtcggg ccgacttcgc caagctcttc gcgcgcaacg ctcatcaggc
    12661 ctacctgcgc tggggcgcaa atgcgaaagc cagtcacctc gagcgggaat tcccgagcat
    12721 gctcgacgat gcgctgcatc ctgcgaccgt gatcggagac ctgaccgacc tcaccgtgcg
    12781 cgacttcagc tccatgtcca tccggtcgag cctggatgga agcgagatca acgcgaacgt
    12841 cctggacacc accacggtgc tgcgcgcggc tcaaaccatc tccagcgaga tcgtcctcga
    12901 ccgcgtcctg acgaagctgc tgcgcctggc gctggagcat gccggcgccc agaaagcctg
    12961 catgctgctc gcccacgacg gaaggctgtt cgtcgaagcc atcgcctcgg tggacggcgg
    13021 cccgacccgc cgcgagagcc cggcgtcccc gctggaggcc tccgacgacg tgcccgagag
    13081 catcgttcac ttcgtggcgc gcaccaagca ggcgctggtg cttggcgacg ccaccgcgga
    13141 ggacgtcttt acccaagatc catacatcgt tcgcaaacag ccgctgtccg tcctctgcgt
    13201 gcccatcgtg caccgcaacg agatcaccgg aatcctctac gtcgagcacc gctggctcac
    13261 cggcgttttc actgcccagc gggtggaagt cctcgccctg ctcgcatctc aggcggcgat
    13321 ctcgatcgag aacgcgcggc tgtacgccga cctgcacgac actcaggagc agtaccgagc
    13381 gctgtacgac aacgccatcg aagggctctt ccggatcgac cccgacggcg cgctgctgag
    13441 cgccaacccg acgctggcaa gaattctcgg cttcgacgac gtcaagcagc tgctggccga
    13501 gtaccgtgat ctcatcgacc gcgtcttcct gagccgggaa gacgccagcc acttcctcac
    13561 caacctcgaa gagcacggcc tgctcaacgt cttcgaggcc cagggggtca cgcgcaacgg
    13621 ccgcacgttc tggatggcgc taacggcgcg cctgagccac gaccccgagc agggcgccta
    13681 catcgacggc tctctcgtcg acatctccga gcgcatgaag cgggagcagg ccgacaagca
    13741 ccggcagatc gccgaggccg caacccaggc gaaaagcgag ttcctggcca acatgagtca
    13801 cgagatccgg acgccgatga atgccatcat cggcttctcc aagctggcgc tggagaccgg
    13861 gctcgacccc aagcagcacg agtacctgtc ctccatacgg gccgcatcgg agaacctgct
    13921 ctcgctggtc aacgacgttc tggacttctc gaaaatagaa gcgggcaagc tcaccctcga
    13981 acaccgaccc ttcaagctgg ccgacacgct caaagaggtg gaacgtctgt tccggaccga
    14041 ggttcgcaaa agcaatctcg agctcaactt cgacgacaac accgcggcgc acgccgagtt
    14101 tcccggcgac ggcgtgatca tcggcgaccc cctgaggctt cagcaggtgc tggtcaatct
    14161 ggtcagcaac gcgctgaagt tcaccgagga ggggagcatc tcggtcacgg cccaggtctg
    14221 tccctgcgag gccggcgagc tgcgcgtcga gttcgccgtc agagacaccg ggatcgggat
    14281 caaacccgag caccaggcac gactcttcga gtcgttcgag caggcggaga cctccaccac
    14341 ccggcgctac gggggcactg ggctggggct gacgatctgc aaacggctgg tgtcgctgat
    14401 ggggggcgag atgtccgtgg cctcggtgcc cgaccaggga agctgcttca ccttcaccgc
    14461 cgtgttcggc cttcgggacg ctcaggacct gatgcccgca gacgtgcgcc agcggcggcg
    14521 caacctgcgc gcgctaaatc gcagagagtt cctggtcgcg gaggacaacc ccatcaacca
    14581 gcaactgtcc ctggagttcc tgcagcgcac cggggcacgg gtggacattg cccagaccgg
    14641 gcgcgaggcg gtggagcgcg cgaccagcaa agcatacgac gcgattctca tggacataca
    14701 catgcccgaa ctggatgggc tccaggcatg ccggatcctg cgcgagcagc agatcacgac
    14761 gcccatcatc gcggtctccg cggacgccat cgaagccagc cagcaagcgg cgctggaggc
    14821 gggctgcaac gcctacgtgt cgaagcccat cgacttcgac ctgctgctgg ccgagctgtc
    14881 ccgggtgctg cccgagcagc cggcggacgc cgctagcgtc gatgctccgg cgtccgcgcc
    14941 tgccgagcac gaagcgtcaa cgacgccggg cgagcttcct gagattcagc tgcctggaat
    15001 cgacctggac acggccatca ggggacacaa cggcaacgtc cgcctcatgc tgaaactcat
    15061 gggggatttc ggcaggtact acggcgacgc cggggcaaag atgcgtgccc acgtgaccga
    15121 caaccatctc gaggaggccg agcggctcgc ccacaacctg cacggcgtgg ccggcagctt
    15181 cggggccgac cgcctcaagc aggcttcaaa gaccctcgag ctcgctctgg ccaagggcga
    15241 cgagagcaac ctgctcgggc tggttcagag tttcgaggtg gccatgaacg aggtgctgga
    15301 atcggcagag accctggcca accacccctt caccgaggtg gcatcgcgtt cacgcaacga
    15361 ggggtgagat cgagcagctg aagcttctcg acgccatcgt ccgaggaaag gccggcgaaa
    15421 tcgaaaaggc cgcggtcgcc gagatgggac ggcaccacgg cactgaggct gcgggcaata
    15481 ttctccacgc gtccgggctg ctcggcgtcc cagcgggcga gcatcgactt gatcacctgc
    15541 cgctgaagat tctcctggga accgcagagg ttgcagggga tgatgggata ctctcggtgc
    15601 tcggcccagc gggcaatgtc gcgctcccgg cagtagtaga gcggtcgtat caccacgttg
    15661 cgtccgtcgt cgcttttcag cttcggcggc atggccttca gccgcgcccc atggaacatg
    15721 ttgagaaaca gcgtttccac cacgtcatca aggtggtgtc ccagcgcgat cttgctcgcg
    15781 ccgatgctcg cagcgaagtt gtagagaatg ccgcgccgca gccgcgagca aaccgggcag
    15841 gtcgtcttgc ccgccggagt gaccgccttg acgatggaat acgtgtcctg ttccaggatg
    15901 tgataggaaa cgtccagccc ttccaggtac ccgggcagca cgtccgccgg atacccgggc
    15961 tgcttctgat ccaggttaac ggcgacgatc tcgaaggcga ccggcgcgct tctctggaga
    16021 ttgagcagaa tgtccagcag cgtgtaggaa tccttgcctc ccgacaggca gaccatcacc
    16081 cgatcgcctt cctcgatcat tccgtaatcg gcgatggcct ggccaacttt gcgcctgagc
    16141 tttctgtgca gcttgttcat gtcgtagccg tgcttgcgca ttgcgcgctc cgacctcgcg
    16201 gcggggtcca ttgggtctgc cttcatcagg gccttgggat ggcgtgggaa cggattttta
    16261 gcagcacgga tcggcgctga caactcacga cgggtcaatc cagaccgcgc agcaccatga
    16321 cggggggcgt agagatcagc ccgcgcgtgc ccagggtccc gacgatgagg atgagcacgg
    16381 acccgatcac cggacccagg agccacagcc agggatgcag acgcaccggc agctcgaaaa
    16441 cctggctttg caggatggcc accgtggctt ccgccccgac cacggcgacc gtgccggcaa
    16501 acaggccgag cgcggcgaat tcggcgacca gcgaccccgc gatgaggcgg cggctgcccc
    16561 ccagcgtgcg gaccagcgcg agctcactca tgcggccgtc gcggctggcc tggatgctgg
    16621 ccaccagcac caggcatccg gacgccacca ccagggcgag cacgagctca acggcctggg
    16681 tcactctgag gatgatgctc tgaacctgag caatgatggc atcgacttcg atgacggtga
    16741 ccgtgggaaa cctggccagg aaccggttga gaaacggctt ctcgtcccgc tcgagatgaa
    16801 agctggtcat ataggtagcg gggaaatcgc tgagcacctg gggagcgaag atgatgaaga
    16861 aattggggcg catgctctcc caatcgagcc gccgcaggct ggcgacttcc acctccacga
    16921 agcgaccggc aatgtcgaag cgcagccggt cgccgacgtc cagcccaagg ctctgggcat
    16981 actcgtcctc gagggaaacc agggcccggg cctcgtccgg cgaccaccag cgtcctttga
    17041 ccacctgatt gtcgtccggc aggtcggcgg cccaggtgag atttcgctcc gactgaagtc
    17101 gcgggcccgg cgcgtccacc ggttgatggt cccgctccca cgcgtcggca tccaccccgt
    17161 tggcggccac gatgcggccg cggatcatgg gaaacaggcc cccgtggctg cccgtaaaat
    17221 cgtccagcat ggtcgcgagc tcgtccacct cgtgcggaac gatgttgaga acgaaatggt
    17281 tgggcgcgtc ttccggcacc tgtccctgcc actcgtcgag caacgacgag cgaagcagga
    17341 ccaggatcag cagcaacatg atggcgagac cgaaaatcag tatctgggcc acgttctccc
    17401 gacggcgacg ctgcagaccg gccagcgcca gccgccaccc gctgcccgcc tgcatgccga
    17461 ccgcgcgacc gccgcgcagc agcagcatcg cgagagcccc gaataccagc aggacgacca
    17521 cggtgccgag tatggtccac gccgtgaggg acgcgctgcc gctgtaccag atcagcaggc
    17581 ccacgcttcc cgccaccgca gccccgtaac tcagatactg ggacggcggg gcagcaccga
    17641 ggtccctgcg aatcactcgc atgggagaga cgttcttgag atgcagcaag ggcggcaggg
    17701 caaacgccac cgcgcagatg aggcccgtgg cggcgcccag caggaacggg cgcgcgctcg
    17761 gcatcggcag atccaccggg atcagagact gcagcaaggc aacgatcccc aggtgcaggg
    17821 ccgcgccccc ggtcaggccc agcacgacgg cggcgcttcc tacggcgagc aggagcatga
    17881 ggtagccgga aaggatctga ttcggcgtcg cgcccagggt tttcagaatg cccacatgat
    17941 cgtagtggcg aagcgcgtag cgatgcgcgc tcaatgcgac cgccacgccg gccagcagaa
    18001 cgccgagcaa accaccgagc agcagaaagc tctccgcgcg agccagggcc gagccgatgg
    18061 agggactgct ttcgcgaatg ccctgccaac ggtagttggg ggcgagcggc agctcggcct
    18121 tgaggctctc gagatcggat tccgcaccac gcagcagcag gcgataggca atgcggctgc
    18181 cgggctgcac gacctgcgtg gcgggcacgt cgtccagccg catgagcagg cgcggcccca
    18241 ggtcgtacat gctgccgccc cgatccggct ctgaggtcag cacctttgcg accttgagcg
    18301 gggcaacgcc tacctcgatc gagtcccccg gtcgaattcc cagcgcgggg aagaggcgcg
    18361 catccaacca gacctccccc ggcgccggta tgtcagatgt cggcacgccg ggcgcaaagg
    18421 gctcatcggc gatcttcagg acgcctcgca gcgggtagcc tgctccaacg gctttgacgc
    18481 tgaccagctg gctgttgtcg tcaccgacga aaaccatgga ggggaaggac atggtgtcag
    18541 cgtgctccac gcccttggcc gacgccgcaa gccggaactc gtccgggatc gggcggctgc
    18601 cgctgatgcg tctgtccgcg gcgagaaagt cggcggattc atcgatgagc gcctggtgca
    18661 gtcggtctac gaagaggctc acggccgtta ccgtccccac cgccagcatc agggcgacga
    18721 gcaggagacc gagctccccg ccacgccagt cgcgccaggc cagccgcagc accagcttga
    18781 tgctcacgag gtaatcctgc ctgccacgat ctccaccacc cgctcacaac gggaggcgag
    18841 acgatcatcg tgggtgacga gaaccagcgt ggtggcgtac tcggcgttga gctcgaagag
    18901 caggtcgatg atgcgctggc ccgtggcagt atccagattt ccggtgggct catccgcaaa
    18961 cagcacgttg ggccgggaag cgaaagccct cgcgatggca acccgctgct gctcaccgcc
    19021 ggaaagctga gtcggatagt gccccgtgcg cgcgccgagc ccgaccttgt ccagcacccg
    19081 cagagcttcg ggctgcgcat ccccatcgcc gcgcagctcg ccgggaagca tgacgttctc
    19141 caacgcggtc aggctgcgca gaagctgaaa agtctgaaag acgaacccga cctgctcgcc
    19201 gcgaacccgc gccctctgct cctcgtcaag ggccgtcagc tcctcaccga cgagatagac
    19261 ccggccgctg gtcggcaggt cgagcccggc gagaataccc agaagcgtgg tcttgcccga
    19321 gccggaggcg ccaacgatgg cgacggtttc tcccgcgttg atttcgaggt cgatgctctc
    19381 caagatggag agttccccct cactcgtcgg cacgcttttg gacacccctt cggtacgaat
    19441 taccggctgg ttcgtcaaat cggtattcct gaccctgatg attgcgctga tcgcgggcgt
    19501 ctcgccggcc agcgcgcggt cgaaaattct ggtgatgggc gacagtatca gcgctgcgta
    19561 cggtattcaa cgcgacgccg gctgggtcgc cctgctggac cggcgactca gcgaaaccgc
    19621 aagcccgtgg caggtggtca acgccagcat cagcggcgaa accacgggcg gagggctggc
    19681 tcgcctgccc aaggccctcg aagctcacga tcccgagatc gtcatcatcg agctgggcgg
    19741 caacgatggt ctgcgcggct acccggtgag ccgcatacgg gaaaatctcg acaagctcgt
    19801 ggagctggcc gtgacgggcg ggcgcagggt gctcctgctc ggcatgagga tcccgcccaa
    19861 ctacgggccc cgctatacgc gcgctttcga ggacatgtat cgcgacgtcg ccgaggccca
    19921 cggcgtgcgt ttcgtgcctt tcatcctgga ggcggtggcc ttgaacccgg gcctgatgca
    19981 ggacgacggg atccatccaa cggctgaggc gcaacaaaag ctgctggatt ccgtctggcc
    20041 cgagctgcag caggtgctgg acggctagcc gttctttctc ccctggctcc cctggcggac
    20101 gccgatgtgc gcctcaccac ggcgagcggc cagcgccacc tgccgggccc gctcggcgag
    20161 cgcggctcgc cgggcctcgg gctgctcctg ggcgcaatgg gggcaggaaa tgccggggcg
    20221 gtagtcgggc gattgcaggt catcggtcga caacggccgc cgacaggcaa agcactgctc
    20281 gtacgagccc tcccgaagat gccggtcgac ggagacgcgc tggtcgaaca caaagcattc
    20341 gccttcccaa cgattcgcct ccccttttac cgtctccagg taattgagga tgccgccgtc
    20401 gagctgatat acctcggcga agccacgttc gagcagatag gcagaggcct tctcgcaacg
    20461 aatgccaccg gtgcagaaca tggcaaccct ggtgtcgcgg tcggggtcga ggtggtcggc
    20521 gacgaagtcc gcaaactggc ggaagctctg cgtcgccggg ttgacggcac cgggaaagga
    20581 gccgatcgca acttcgtagt cgttgcgcgt gtcgacgacg gtcacctcgg ggtcgtcgag
    20641 cagttcgttc cattcctcgg cacccacatg ggtgccggtc cggccacccg ggtcgaggcc
    20701 ggaaacgccg aagctgacga tctccggctt gacgcgaacc ttgagccggt ggaacaccgg
    20761 attgcccgca gccgcggaag aatacttgac cgccaggccg gagaattccg gcaaccgctc
    20821 gagggcgccc agaaatttct gcagagccac ggcatttccc gtgaccgttc cgttgatgcc
    20881 ttccccggcg ag
//
LOCUS       DBNHQR010000132         2443 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_202410, whole genome shotgun sequence.
ACCESSION   DBNHQR010000132 DBNHQR010000000
VERSION     DBNHQR010000132.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_202410"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="AC16VR_02925"
     CDS             <1..666
                     /locus_tag="AC16VR_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012402.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR6010137.1"
                     /translation="EEPGCRWTVFYGNRSAGRAMFLDELLGLKDRYLGRLALHFVMSR
                     EPSEAEMFNGRIDAAKVEASAGKLFDPAATDEFFLCGPGDMIAAVGAALGKLGVAPDR
                     IHSEHFTVESVAAADRDGEPAPRTGGAAEIIVIMDGRRRSFTMSPDDESILDAALDSG
                     IDLPYSCCAGVCSTCRTRVVEGAAEMAENFSLEDWEIENGYVLACQSRPTSSELVLDY
                     DDV"
     gene            712..1581
                     /locus_tag="AC16VR_02930"
     CDS             712..1581
                     /locus_tag="AC16VR_02930"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6010138.1"
                     /translation="MGNVGFVGLGVMGYPMAGHLARAGYNVRVYNRTGAKSERWVTEH
                     GGSRVETPREAASGADVVCLCVGNDDDVRAVVYGPEGVLDGLADGSVLIDHTTVSAEL
                     AEELASAAGERGCGFLDAPVSGGQAGAENGQLTVMVGGEQTIFDSALPVMEAYGKSVR
                     RMGPAGTGQLTKMVNQICIAGVLQGLAEGLHFADQAGLDIESVVDVISKGAAQSWQME
                     NRHATMARGEFDFGFAVDWMRKDLAIALQAGRRLQARLPLTALVDQFYADVQSYGGGR
                     WDTSSLIQRLRRT"
     gene            1708..2130
                     /locus_tag="AC16VR_02935"
     CDS             1708..2130
                     /locus_tag="AC16VR_02935"
                     /inference="COORDINATES: protein motif:HMM:NF013319.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HIR6010139.1"
                     /translation="MSDDRAIASWSHFDHAADMGVRGVFARCFPAARLEMLFDVSHNT
                     CKVETHRVGDETRELFVHRKGVTRAFGPGHPSLPDPLRSVGQPVLIGGSMGTESYVLR
                     GTKESERRAYSSACHGAGRAMSRRAATRRWRGWFPCSA"
     gene            2236..>2443
                     /locus_tag="AC16VR_02940"
     CDS             2236..>2443
                     /locus_tag="AC16VR_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010140.1"
                     /translation="MYVLLFVALLGCGAWLAHSGSRHHRKVQVWGGAAIVGSAFGLML
                     LMAFWAEMLWFDSLGYQDRFWTFVW"
BASE COUNT          380 a          738 c          875 g          450 t
ORIGIN      
        1 gaagagccgg gctgcaggtg gacggtgttc tacggcaacc gcagcgcggg ccgggccatg
       61 tttctcgacg agttgctggg cctcaaggat cgctacctcg gccggctcgc cctgcacttc
      121 gtgatgagcc gtgagccgag cgaggccgag atgttcaatg gccgcatcga cgcggccaag
      181 gtcgaagcaa gtgccggcaa gctcttcgac cccgccgcga cggacgagtt tttcctgtgc
      241 ggccccggcg acatgatcgc ggcggtcggc gccgccctgg gcaagctggg cgttgccccg
      301 gaccggattc acagcgagca cttcacggtg gaaagcgtgg ctgcggcgga tcgcgacggg
      361 gagccggctc cacggacggg tggcgcggcg gagatcatcg tgatcatgga cggccgtcgc
      421 cgatcgttca ccatgagccc ggacgacgag tccatcctgg atgccgcgct ggattccggg
      481 atcgatctgc cttactcctg ttgcgccggc gtgtgctcca cgtgtcgtac ccgcgtcgtg
      541 gaaggtgcgg cggagatggc tgagaatttt tcgctggaag actgggaaat cgagaacggg
      601 tacgtgctgg cctgtcagtc ccgcccgacc agcagcgagc tggtgctcga ctacgatgac
      661 gtgtaggcag ccagtgtggc tccaagccag aggaggtgga tggtgagcgg gatcggcaat
      721 gtgggtttcg ttggcctcgg cgtcatgggt tatcccatgg ccggacacct ggcccgcgcg
      781 ggctacaacg tgcgcgtgta caaccgaacc ggcgccaagt ccgagcgatg ggtaaccgag
      841 catggcgggt cccgggtcga aaccccgcgg gaggctgcgt cgggggcgga cgtggtctgc
      901 ctttgtgtgg gcaacgacga tgacgtgcgc gccgtcgtct acgggcccga gggtgtgctc
      961 gacgggctgg ccgacggctc ggtgctgatc gaccacacca cggtctccgc ggagctggcc
     1021 gaggagctgg cttctgccgc cggcgagcgc ggttgcgggt ttctggacgc gcccgtctcc
     1081 ggaggtcagg cgggagccga gaacggacag ctgacggtga tggtgggcgg ggagcagacg
     1141 atcttcgaca gcgctctgcc cgtcatggaa gcctacggca aatcggtccg tcgcatgggt
     1201 cccgctggca ccgggcagct caccaagatg gtcaaccaga tctgcattgc cggggttctg
     1261 caggggctgg cggaggggct gcactttgcc gatcaggccg ggctggacat cgaatcggtg
     1321 gtggacgtga tctccaaggg cgccgcccag tcctggcaga tggagaaccg gcacgccacc
     1381 atggcccggg gcgagttcga tttcggtttc gccgttgact ggatgcgcaa ggatctcgcc
     1441 atcgcgcttc aggccggacg tcggctgcag gcccgtttgc cgctcacggc actcgtggac
     1501 cagttttatg ccgacgtgca gagctacggt ggcggccgtt gggatacttc cagcctgatc
     1561 cagcgtttgc ggcgcacctg accaccttgg atcgccacct cggacatccc ctatgttcga
     1621 gctttcggct acgcggtagc gtcggaaatc gtcgccgatc acggcatttg ttgcgcgcgg
     1681 caaccgtgcg aatcatgggg ctgacagttg agcgacgatc gggccatcgc gagttggagc
     1741 cacttcgatc atgcggccga catgggggtt cgcggggtgt tcgccagatg cttcccggca
     1801 gcgcggctcg agatgctctt cgacgtctct cacaacacgt gcaaggtgga aacccaccgg
     1861 gttggcgacg agacccggga gctgttcgtt caccgcaagg gtgtcacgcg agcgtttggc
     1921 cccggtcacc cgagcctgcc cgaccccctg aggtccgtgg ggcagccggt gctgatcggc
     1981 ggcagcatgg gaaccgagtc ttacgttctg cgcgggacga aggaaagcga gcgccgcgcc
     2041 tactcctccg cctgtcatgg ggcgggtcgg gccatgagcc gccgcgcggc cacccggcgc
     2101 tggcgcggct ggttcccgtg ctctgcgtga agggctaacg gcgcccgaca cggccttaag
     2161 gtctaggtag taaccacggt tgcccaggcg aacccgcctg ccagactgga acggtcggca
     2221 agaagggtgt gaaccatgta cgtcctgctt ttcgttgctc tgctgggctg tggagcctgg
     2281 ctggctcatt ccggcagtcg gcatcaccgc aaagtgcagg tctggggcgg tgcagccatc
     2341 gtcggttccg cgttcgggct gatgttactg atggctttct gggccgagat gctgtggttc
     2401 gactcgctcg gctatcagga tcggttctgg accttcgtct ggg
//
LOCUS       DBNHQR010000133         2245 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1279349, whole genome shotgun sequence.
ACCESSION   DBNHQR010000133 DBNHQR010000000
VERSION     DBNHQR010000133.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1279349"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="AC16VR_02945"
     CDS             <1..958
                     /locus_tag="AC16VR_02945"
                     /inference="COORDINATES: protein motif:HMM:NF024703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HIR6010141.1"
                     /translation="DNGFEAEDWLPAVGQLETRALATMALLEDFGRGAAEFSKDGSPQ
                     GLKARWVNETAGDLEMVSAPLQPGQLLQEALWSSCYAAVCTSATLTALGTFDRFLEQV
                     GLGSVNAVRIPSPFDFQRLATLTVPSMRSDPREFEAHSQEVAALLPGLLADDPGALVL
                     FTSWRQMHEVIGQLPEDLLDKLQVQGEASKQALLSRHRSAVDEGRQSVLVGLASFAEG
                     VDLPDDYCRHVVIVKLPFAVPDDPMDQAMAEWVEARGRNAFFEIAVPDAALKLVQACG
                     RLVRHEGDFGRITLLDRRIVTARYGRALLDSLPPYRRELNPS"
     gene            complement(945..>2245)
                     /locus_tag="AC16VR_02950"
     CDS             complement(945..>2245)
                     /locus_tag="AC16VR_02950"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HIR6010142.1"
                     /translation="ELLAADGDGPRLAIDDAGLSLWLDRDTEFGTPRANVYLLLGLRD
                     GLESPRDIAMAQLYQRLVNDASNQLAYPAWLAGLSYRLSLAPQGFQLIISGYSDKQAA
                     LLDAVIENLTDVPIDAAKFELYQAELVREWRNFAQERPVTQTYSALWFLLLSSSWPPE
                     ALASALENAKPEDLAAWRSKRLDVFDVTGLAHGNVDAAAIDDIADLIREHLPQQGFGL
                     SQPQVRTVAESLKLAVPVDHPDAAMILHVQDPDTSYEARARSVMAAHLLSEPYFTSLR
                     TEQQLGYVVDITARALRRRGAITFLIQSPVASPAQLEAATRQFVTDQLAALQDMPTEQ
                     FEESRAGLIARLTEQDSNLGQRSQRYWADIETGVTSLDSRERIAEAVNGLAVSDMIAY
                     LKNLQQKLDDQRLLIYHDGQFPPGPLGGREIESVQAFKRG"
BASE COUNT          341 a          706 c          804 g          394 t
ORIGIN      
        1 ggacaacggc ttcgaggcgg aggactggct gccggccgtg gggcagctgg aaacgcgcgc
       61 gcttgcgacc atggctctgc tggaggattt cggccgcggc gcggcagagt tttcaaaaga
      121 cggctcgccc cagggattga aggcccgctg ggtcaacgag accgcgggtg atctggaaat
      181 ggtcagcgcc ccgctgcagc ccggccagct tctgcaggag gcgctctggt cgagttgcta
      241 cgcggccgtg tgcacctcgg cgacgctgac tgctctgggc accttcgacc gctttctgga
      301 gcaggtgggc ctgggttcgg tcaacgcggt ccggattccg agcccattcg actttcagcg
      361 gctggcgacg ttgacggtgc cgagcatgcg ctcggacccc agggagttcg aggcgcacag
      421 ccaggaggtg gccgcgctcc tgccgggatt gcttgccgat gacccgggcg cgctggtgct
      481 gttcacgtcc tggcggcaga tgcacgaggt gatcggacag ctcccggagg acctgctgga
      541 caagctgcag gttcagggcg aggcctccaa gcaggcgctt ctatcccggc accgcagcgc
      601 ggtggacgag ggtcggcaga gcgtcctggt cggccttgcc agcttcgccg agggtgtcga
      661 cctgcccgac gactactgcc ggcacgtggt catcgtcaaa ctgccctttg ccgtgccgga
      721 cgatccgatg gatcaggcca tggccgaatg ggtcgaagcc cgcgggcgca acgcgttctt
      781 cgagatcgcg gtgccggacg cggcgctcaa gctggtccag gcatgcgggc gcctggtcag
      841 acacgaagga gacttcggcc ggatcacgtt gctcgacaga cgcatcgtca cggcgcgtta
      901 cgggcgtgca ctgctcgact cgctgcctcc ctacaggcgg gagctcaacc cctcttgaag
      961 gcctggacgc tctcgatctc cctgccgcca agggggcccg gcggaaattg gccgtcgtga
     1021 taaatgagca gtcgctggtc gtccagcttc tgctgaaggt tcttcagata ggcgatcatg
     1081 tcgctcacgg cgaggccgtt cacggcctct gcgatccttt cccgggagtc gaggctggtt
     1141 acgcccgttt cgatatccgc ccagtagcgc tggctgcgct ggccgaggtt gctgtcctgc
     1201 tcggtcaacc gcgcgatgag gcccgcccgg ctctcctcga actgctcggt cggcatgtcc
     1261 tgcagggcgg ccagctgatc ggtgacgaac tggcgggtgg ccgcctcgag ctgagccggg
     1321 cttgccaccg gtgactggat caggaaggtg atggcgcccc gtctgcggag cgcccgagcg
     1381 gtgatgtcca ccacgtagcc gagctgctgc tccgtgcgca ggcttgtgaa gtacggttcg
     1441 ctcaggaggt gcgcggccat cacgctgcgg gcgcgcgcct cgtagctggt gtccggatcc
     1501 tgcacgtgaa gaatcatcgc cgcgtccggg tggtccacgg ggaccgcgag cttcagtgac
     1561 tcggcaacgg tgcggacctg gggctggctg aggccgaaac cctgctgggg caggtgctcg
     1621 cggatcagat cggcgatgtc gtcgatggcg gcagcgtcca cgttcccgtg cgccaggccg
     1681 gtcacgtcga aaacgtcgag cctcttgctg cgccaggccg ccaggtcctc cggctttgcg
     1741 ttctccaggg cagatgcgag cgcctcggga ggccagctgc tcgacagcag cagaaaccag
     1801 agcgcgctgt aggtctgggt gaccggacgt tcctgggcga agttgcgcca ctcccgcacc
     1861 agttccgcct ggtagagctc gaactttgcg gcatcgatgg gtacgtcggt gaggttttcg
     1921 atcaccgcgt cgagcagggc ggcctgcttg tcgctgtaac ccgagatgat gagctgaaaa
     1981 ccctggggtg cgagagacag tcggtagctc aggcccgcga gccaggccgg gtaggccagc
     2041 tggttgctgg cgtcgttgac gaggcgctga tagagctggg ccatggcgat gtcgcggggg
     2101 gactcgagcc cgtcacgcag gcccagcagc aggtagacgt tggcccgtgg cgtgccaaac
     2161 tcggtatcgc ggtcgagcca cagcgacagg ccggcgtcgt cgatggccag ccgcggcccg
     2221 tcgccgtccg cggcgagcag ctcga
//
LOCUS       DBNHQR010000134         5771 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1215890, whole genome shotgun sequence.
ACCESSION   DBNHQR010000134 DBNHQR010000000
VERSION     DBNHQR010000134.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5771)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1215890"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..824
                     /gene="hisN"
                     /locus_tag="AC16VR_02955"
     CDS             9..824
                     /gene="hisN"
                     /locus_tag="AC16VR_02955"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004374316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HIR6010143.1"
                     /translation="MSDSERVEYLEFALGLAAAAGSAILPFFRAGAGVENKRTDGGFD
                     PVTEADHAAERVIREQILGRYPEHGIFGEEFGLDAGNGLTWVVDPIDGTRAFMSGLLH
                     WGVLLALFDGERPVLGVMHQPFTAEFFAGDCHNAHYHRGETSQALEVRECKGLGQAVL
                     GTTSPKLFTSPFERRGLDAIEERVKLSRYGGDCYLYAMLAMGQLDIVMDAGLQPYDVQ
                     ALVPIIEGAGGVVTTAQGGDASMGGFVVATGSAELHAEVLATIRAGSELGPGS"
     gene            complement(1137..1976)
                     /gene="surE"
                     /locus_tag="AC16VR_02960"
     CDS             complement(1137..1976)
                     /gene="surE"
                     /locus_tag="AC16VR_02960"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014077.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HIR6010144.1"
                     /translation="MFAFAAQPCTDRPLNILLVNDDGFDTPGIIALHRELKASGHNVR
                     RVAPDRNYSGSSASLTIDLIAAPRIPDEEFTEVYAVSGSPATAVLVGATAIFPPEQPV
                     DLVVSGINEGANLGPATVISGTVGATIAAMKALARPIPGIAVSTNPIAEDEAAPENHR
                     HLKTVAGFTSRLVRRLFCASQPWSKGELALNVNYPPLEPEAVKGVRVAAQGKAPYYSM
                     GFKAAAPDRYAPDFGRAEPSQDIENADTFLFRQGYITIVPIDGDYTAGGRPLPASLED
                     LEP"
     gene            2099..2857
                     /locus_tag="AC16VR_02965"
     CDS             2099..2857
                     /locus_tag="AC16VR_02965"
                     /inference="COORDINATES: protein motif:HMM:NF024258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR6010145.1"
                     /translation="MASLELMDANQLDYSPIRRAGVIGDVHAEHERLAGALEWLSGQD
                     VDVILCTGDVADGVGCVNTACRLLRDADVLTVAGNHDRWLLQDRVRHVADAHFLADLD
                     DESRDYLTALPRSRTLNTVAGPLLLCHGVVENDLAKVWPGTERSPIERSTEMDDLVAA
                     STHRFVVNGHMHYRVLIDFEGVVLVNAGTIRGPFSGVTVIDFEGDFAAAYERDGNGRA
                     HRVAEHELTRNGERRIWRDTQEFDGSWQPVTLHP"
     gene            complement(2886..3482)
                     /gene="purN"
                     /locus_tag="AC16VR_02970"
     CDS             complement(2886..3482)
                     /gene="purN"
                     /locus_tag="AC16VR_02970"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HIR6010146.1"
                     /translation="MRIAALASHGGSILQAVIDAIENGTLPAELVLVVSNNSRSQALA
                     RASRHGIATLHLSAHTHRDPDELDAAIVRSLEEANADWVLLAGYMKRLGPRMLGRFRN
                     RIINTHPALLPKFGGQGFYGQRVHEAVIEAGETESGATVHVVNEAYDSGPILAQVRVP
                     VEKNDTAEVLEERVKRAERKLIVTTLAELAARPAASGY"
     gene            complement(3482..4255)
                     /locus_tag="AC16VR_02975"
     CDS             complement(3482..4255)
                     /locus_tag="AC16VR_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010147.1"
                     /translation="MTTYEVVARNFSQAHENRIHSDEVAQRYGFTGALVPGVAVYGHL
                     THPVVERLGEDWLGRSVSSVRFLKPAYDGDRLHLDCESRGSALLVSCRNDAGTLLAEL
                     HTEQPRDMPEPVTAAVLDEPRRTAERAEISWEAVVENETFEPWHWQITEDGNAQYAAE
                     VADHLPIYRLCAHPHWLLSTANRALTREYAMPAWIHVGSDVRHRALVKVGDTVEVRAT
                     LMEKWRRKGHEFLRLYVAYRRGRELTTEIFHTAIFKVAA"
     gene            4426..5340
                     /locus_tag="AC16VR_02980"
     CDS             4426..5340
                     /locus_tag="AC16VR_02980"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HIR6010148.1"
                     /translation="MAALRGVRLVVVTGKGGVGKTSVAAGIASASARSGLRTVLVTLD
                     TGDTRHPLLDVPLEYQPYQAAPRLSVSRLDALEAVREYVRRNMPFSSLYENIFDGHAF
                     RNFASAAPGFEELMSLGKLYELCTSGEFDRVVFDAPATGHMKLLLDVPGATLRAVHVG
                     PLNHNAGKIQDLLLDGSRTRVVITTLAEEMAVEEAMDLERCCRNDHRMAVGPIVVNRL
                     VPERFSVEEIAALQSMEASTLELREAVVAVVAEHAEQRSQEEALRALSAAGSDVVSVP
                     RIVQACFDPAAFLEALEAHLMPLFGNAA"
     gene            5337..>5771
                     /locus_tag="AC16VR_02985"
     CDS             5337..>5771
                     /locus_tag="AC16VR_02985"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014433.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HIR6010149.1"
                     /translation="MSAALKLEGVVADRRIIICCGSGGVGKTTTGAALGVLAAQLGRS
                     ALVMTIDPAHRLAQAMGLQALGHEPAEIALEAPGRLSAMMLDTKHTFDRLVEQYAPSG
                     QVRDAIFANEYYRQLSSTLGGSRELVAMERVLEAATEEDYDLL"
BASE COUNT          956 a         1722 c         1951 g         1142 t
ORIGIN      
        1 cgcggcagat cagtgattcg gagcgggtcg agtatctgga gttcgctctg gggctggcgg
       61 ccgcggctgg ctctgcaatt ctgccgttct ttcgcgccgg cgcgggtgtg gagaacaaac
      121 gcaccgatgg tggattcgac ccggtaacgg aggccgatca cgctgcggag cgcgtgatac
      181 gtgagcagat tctcggccgg tatcccgaac acgggatctt cggagaagaa ttcggtttag
      241 acgcgggtaa cggtctgacc tgggtcgttg atcccatcga tggcactcgg gcgttcatga
      301 gcggccttct gcattggggg gtgctgctgg cgctgttcga tggcgagcgt cccgtcctgg
      361 gcgtcatgca tcagcctttt accgcagagt tttttgcggg cgattgccac aacgctcatt
      421 accaccgggg tgaaacctcc caggcgctcg aagtccggga gtgcaagggg ctcgggcagg
      481 ccgttctcgg cacgacgtcg ccgaagcttt tcacgtcgcc attcgagcgg cggggcctcg
      541 acgccattga agaaagggtg aaactcagcc gctacggtgg ggactgctat ctgtatgcga
      601 tgcttgccat gggtcagctc gacatcgtca tggatgccgg cctgcagcct tatgacgttc
      661 aggctctggt gccgatcatc gagggcgctg gtggggtcgt gaccactgct cagggcggtg
      721 acgccagcat gggcgggttt gtggtggcta ccggcagcgc ggagcttcat gcagaggtgc
      781 tcgcgaccat ccgtgcgggc agtgaactcg gccctggttc ctagattcga atcgagtggt
      841 tcagatcagc ggcccgtcat cgacgggccg gaatgtcgag gggacgtccg tgtcctgatc
      901 gtcggttcgg cacgccgggc tgatgtgacc cgaaaacgcg actaaacggg tcgatcagag
      961 cggaccgata ctgctggtcc gctcctcgag ggcccggcgc gaaccggctg gggcctcgag
     1021 cgtctagact gccaccgacc gcctgccgaa gtgtgacgac aatgtcgggg ttttgtagcc
     1081 gtttggcgca gtgctcggtc aacgtatctg cggttcctgg accgtcacgg cagaagttac
     1141 ggctcgagat cctcgaggga ggccggaagt ggccgtccgc cggcagtgta gtcgccgtcg
     1201 atgggcacga tggtgatata gccctgccgg aagagaaagg tgtcggcgtt ttcgatgtcc
     1261 tggctcggtt ctgcccggcc gaagtccggg gcgtagcgat cgggggctgc cgctttgaag
     1321 cccattgagt agtagggcgc ctttccctgg gccgcgactc gcaccccctt tacggcttcg
     1381 ggctcgagcg gaggatagtt gacgttcaga gcaagctcgc ctttagacca gggctgcgaa
     1441 gcacagaaga gcctcctcac caaccggctg gtgaacccgg cgaccgtctt cagatgacga
     1501 tggttttccg gcgcggcctc gtcttcggcg atgggattcg tgctcacggc gattcccggg
     1561 atcggtcttg ccagcgcctt catggcggcg atggttgcgc ccacggtccc ggagatcacg
     1621 gttgccggcc ccagatttgc tccctcgttg atcccggaaa cgaccaggtc cacaggctgc
     1681 tccgggggga agatggcggt cgcgccgacc agcactgccg tagcagggct cccggagacc
     1741 gcatagactt cggtgaactc ctcgtccggg attcgggggg ccgcgatgag gtcgatggtg
     1801 agcgatgcgc tgctgccgct gtagttgcgg tccggcgcga cccgtctgac gttgtgcccg
     1861 ctggctttta gttcgcgatg cagagcgatg atcccgggcg tatcgaaccc gtcgtcgttg
     1921 acgagcagta tgttcagcgg cctgtccgtg cacggttggg ccgcgaacgc gaacattggg
     1981 gacagcagaa caattgcgaa gaatcgtcgg tacatgatgc aaacagagga tggccgcaga
     2041 ccatcatgcc acatctctgt gacgggttta gatccgttca cggagacttc acgagtcggt
     2101 ggctagtctc gaactcatgg atgccaacca attggactac tcccccattc gccgcgccgg
     2161 cgtgataggc gacgtgcacg ccgagcatga gcgtctggcg ggggcgttgg aatggctctc
     2221 gggccaggac gtggacgtca tcctgtgcac cggcgacgtc gccgacggcg tcggctgcgt
     2281 caataccgcc tgcaggctgc tcagagacgc tgatgtgctg acggtcgcgg gcaatcacga
     2341 tcgatggctg ctgcaggacc gcgttcggca cgtcgcggac gcccactttc tggccgatct
     2401 ggacgatgaa agccgcgatt acctgactgc gctgcctcgt tcgaggactt tgaatacggt
     2461 cgccggaccg ctccttttgt gtcatggggt ggtggagaac gatctcgcga aggtctggcc
     2521 cggcacggaa cgttcgccga tcgagcgtag cacggagatg gacgatctgg ttgctgcgag
     2581 cacgcaccgg tttgtggtca acggacacat gcactaccgg gtgctgatcg actttgaggg
     2641 tgtggtcctc gtcaacgccg gcaccatcag agggcctttc tccggcgtca ccgtgatcga
     2701 tttcgaagga gatttcgcgg cggcctacga gcgtgatggc aatgggcggg cgcaccgcgt
     2761 tgcggagcac gagctgaccc gcaatgggga gcgaaggatc tggcgtgaca cgcaggaatt
     2821 tgacggttcc tggcagccgg ttaccctgca cccttagagg cgcgccgcga ggccgtcccg
     2881 ttgcctcagt atcccgaggc ggccgggcga gcggctaact cggccagcgt cgtgacgatg
     2941 agcttcctct ccgcgcgctt gactcgctcc tcgagcacct ccgccgtgtc gttcttttcg
     3001 acaggcactc gaacctgggc gagaatgggc ccggagtcat atgcttcgtt cactacgtgc
     3061 acggtggcgc ccgattccgt ctcacccgcc tcgatcacgg cctcgtgaac ccgttggccg
     3121 tagaatccct gcccgccgaa ctttggaaga agtgcaggat gggtgttgat gatcctgttc
     3181 ctgaaccgac cgagcatgcg cgggcctagc ctcttcatgt agccggccag aaggacccag
     3241 tctgcgttcg cttcttccag cgaacggacg atggcggcgt cgagctcatc gggatcccga
     3301 tgggtatggg cgctcaggtg aagggttgcg attccgtggc ggctcgcgcg tgccagcgcc
     3361 tgggatctgc tgttgttgct gaccaccagc accagctccg cgggcagcgt tccgttctcg
     3421 atggcgtcga tgaccgcctg gagaatcgat ccgccgtgag atgccagggc cgcgatgcgc
     3481 atcaggccgc caccttgaat attgcggtgt gaaagatctc cgtggtgagt tccctgcctc
     3541 tgcggtacgc cacgtacagc ctcaggaact cgtggccctt cctgcgccat ttctccatga
     3601 gcgtggccct gacttccacg gtatcgccaa ccttgacaag cgcccggtgc cggacatcgg
     3661 agccaacatg aatccatgct ggcattgcgt actccctcgt gagtgcccga ttggcagtgc
     3721 tgagaagcca gtgggggtga gcgcagaggc gatagatggg cagatggtcg gcaacctcag
     3781 cggcgtactg cgcattgccg tcctcggtga tctgccagtg ccacggctcg aacgtttcgt
     3841 tctcgaccac cgcctcccag ctgatttccg cacgttccgc ggttctgcgg ggctcgtcga
     3901 gcacggcggc ggtgaccggc tccggcatat ctcgcggttg ctcggtatga agctccgcca
     3961 gcagggtgcc ggcgtcattg cggcagctga cgagcaacgc ggaccctcgg ctttcgcagt
     4021 ccagatgaag tctgtccccg tcgtaggccg gcttcaggaa ccgcaccgag ctcaccgacc
     4081 ggccgagcca gtcctcaccc agacgctcca ccacgggatg cgtcaggtgc ccgtagacgg
     4141 cgacgcccgg gacgagggca ccggtaaagc catagcgttg cgcgacctcg tcgctgtgga
     4201 tcctgttctc gtgagcctgg gagaaatttc tcgccacgac ctcgtaggtg gtcatctgtg
     4261 gccctctgtg ccgatggatg cgttgccgca acgtctgctg atcatacctc tgaagctcta
     4321 ggtccgttag gcagatctgt ctgtgatggg tcggctgcgg cgcagtcaag gtcgatccgg
     4381 gtggtgaaag cgcattatag tgcggcgatg aatggtgatg cttcagtggc ggcgctgcgc
     4441 ggcgtgcgcc tggtggtcgt gactggcaag ggcggcgtgg gcaagacgtc ggtggctgct
     4501 gggatcgcga gcgcttccgc caggtcgggg ctcagaaccg ttctcgtcac cctcgacacc
     4561 ggagatacca ggcatccgct gctcgacgtt cccctcgagt atcagcccta tcaggcggcc
     4621 ccgcggcttt ccgtttcgcg gctggacgcg ctcgaggcag tgcgggagta cgtccggcga
     4681 aacatgccgt tctcgtcgct ctacgagaac atcttcgacg ggcacgcgtt tcgtaatttt
     4741 gcttcggcag cacccggctt cgaggagctc atgtctctcg gcaagctcta cgagctctgc
     4801 accagcgggg agttcgatcg ggtggttttc gatgcgcccg ccaccggtca catgaagctt
     4861 ctgctggacg tgcctggggc caccttgcgg gccgtgcacg tgggaccgct gaaccacaac
     4921 gcgggcaaga ttcaggacct gctgctggac ggctcgcgga cgcgcgtcgt catcaccacc
     4981 ctcgcggaag agatggcggt ggaggaggcg atggacctcg agcgctgctg ccgtaacgat
     5041 catcgcatgg ccgtaggccc gattgtcgtc aatcgcttgg tgcctgagcg attttcagtc
     5101 gaggagatcg cggcgctgca gagtatggag gcgagcacgc tggagctgcg cgaagcggtg
     5161 gtggctgtgg ttgccgagca tgccgagcag cgatctcagg aggaggcgtt gcgcgcgctg
     5221 agtgcggccg gcagcgacgt cgtgtccgtg ccgcgcatcg tgcaggcatg ctttgacccg
     5281 gcggctttct tggaagcgct ggaggctcac ctgatgcccc tgttcggtaa cgcggcatga
     5341 gtgccgcgct caagctggaa ggcgtcgttg cggatcggcg gataatcatc tgctgcggca
     5401 gcggcggtgt gggcaaaacc acgaccgggg cggcgcttgg agtgcttgcg gcccagctgg
     5461 gcagatcagc gctggtcatg accatcgatc cggcgcatcg gctggctcaa gccatgggcc
     5521 tgcaggcgtt ggggcacgaa cctgcggaaa ttgcgctgga agcacccggc aggctgtcgg
     5581 ccatgatgct cgataccaaa cacaccttcg accggctggt ggagcaatac gcgcccagcg
     5641 gtcaggtcag agacgccatt ttcgcgaacg agtactaccg ccagctctcc tcgacgctcg
     5701 gagggtcccg ggaactcgtg gccatggagc gcgttctcga agctgcgacg gaggaggact
     5761 acgatctgct g
//
LOCUS       DBNHQR010000135         3267 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_519150, whole genome shotgun sequence.
ACCESSION   DBNHQR010000135 DBNHQR010000000
VERSION     DBNHQR010000135.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_519150"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..528)
                     /locus_tag="AC16VR_02990"
     CDS             complement(28..528)
                     /locus_tag="AC16VR_02990"
                     /inference="COORDINATES: protein motif:HMM:NF024828.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HIR6010150.1"
                     /translation="MNGQSLLAASLIAAVAWSAPAAAQTLASSMGIFIYPTQSQTSDQ
                     QSRDDYECFGFAKTQSGYDPMNPPQITAQAPDQGPSGARVAGAARGAAGGAVIGAIAG
                     DAGKGAAYGATLGAMGGGRADRQRRAQQSQQAEQSAQQQMANMQLTFKNAYGACIEAR
                     GYSVKY"
     gene            complement(655..1329)
                     /locus_tag="AC16VR_02995"
     CDS             complement(655..1329)
                     /locus_tag="AC16VR_02995"
                     /inference="COORDINATES: protein motif:HMM:NF019500.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR6010151.1"
                     /translation="MRPLTSWRIVQALGKQRYLCLSLLAFLFALPLAGDEPGARLTTQ
                     LVLVFVFITGPLAVARTRATLWITGVFAIVVFVMGIGSVLGGGDWFLGTSALVGFVFF
                     GFLSWLVTRELLFDVKEVTAETLWAAVNVYVLIGLSFAFLYSVLALSSPDAFVGKFMS
                     EPLRDQLLGFVYFSFVTLTTLGYGDITPNNTFVGTLTYLEALVGQLYVAIMIARLVGL
                     YISSQK"
     gene            complement(1445..2365)
                     /locus_tag="AC16VR_03000"
     CDS             complement(1445..2365)
                     /locus_tag="AC16VR_03000"
                     /inference="COORDINATES: protein motif:HMM:NF024242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR6010152.1"
                     /translation="MAKHGIVADFELIPDYMVSARLLHGYIIEAVSHGPPGAVSVAAG
                     LFNAAFQPNPFSDTAWKSVTLLEAIRRHNANVTRYSPENRFRFSVAKGEGHWEKQDRS
                     PLAETEIFCVANLIGHVRMSLGDGWSPPSVDVSVAEPSTLASLPLFEGVSVRAVPISA
                     TIHIPLSALALPSSSRDAGLSERALPDDFGDLDFVQSMRELIRSYVRVGELSFSNVAL
                     AAGLSRRTLQRRLSDVGISYSGMVDQVRFEVARELLLESPELPVTQIGYELGYADPGS
                     FTRAFRRFAGTSPIAFRRASPSGMPTAEVA"
     gene            2561..2833
                     /locus_tag="AC16VR_03005"
     CDS             2561..2833
                     /locus_tag="AC16VR_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010153.1"
                     /translation="MSNQIRDADAIRPALFDIEGAIAGYFEWKKQLYDRRGFATSTTL
                     LALYQHADRSISGNKHGAGLIFRWQGSWDLVGRERRRRVSERSALS"
     gene            2830..3099
                     /locus_tag="AC16VR_03010"
     CDS             2830..3099
                     /locus_tag="AC16VR_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010154.1"
                     /translation="MKGMVTDEIWLGEGVYDANASSGEFDWSSVGEGEYLKHLEVGCT
                     PAFARRAKNTVQLTWWEKDRRKDAGTPCVTNGSSSPATNFSSPVP"
BASE COUNT          679 a         1045 c         1009 g          534 t
ORIGIN      
        1 tgtccgcatt cgttcttctc ctttccctta atacttgacg gagtagcccc gggcctcgat
       61 gcaggcgccg taggcgttct tgaaggtgag ctgcatgttg gccatctgct gctgcgcgga
      121 ctgttccgcc tgctgggatt gctgggcccg ccgctgccgg tcggctcggc cgccgcccat
      181 ggctccgagc gtggcgccgt aggctgcgcc cttgcccgca tcgccggcga tggcaccgat
      241 gacggcgccg ccggcagcac cccgcgctgc cccggcaacc cgggccccgg acgggccctg
      301 atccggcgcc tgcgccgtga tctgtggagg attcatgggg tcgtaaccgc tctgggtctt
      361 ggcaaagccg aagcattcgt aatcgtcccg ggattgctgg tccgacgtct ggctctgggt
      421 cgggtagatg aagatgccca tggaagatgc gagcgtctgg gccgctgctg gcgcagacca
      481 ggccacagcg gcgatgagcg aagccgccag tagggattga ccgttcattt agtcctcctt
      541 gaatcctgga gtgggcatga gccctacccc gtgggcccgc ctttatacga tcgctgcaac
      601 gcccactgca agcgcgagtt tccgatgagg ctccgaccta cccgttcccg gaagctactt
      661 ctgcgatgag atgtacagcc ccaccagccg agcgatcatg atcgccacgt acagctggcc
      721 gaccaacgcc tccaggtaag tcagggtgcc gacaaaggta ttattcggcg tgatatcccc
      781 gtacccgagg gtcgtgaggg tcacgaagct gaagtagacg aaccccagca actggtcccg
      841 cagcggctcg gacatgaact tccccacaaa cgcatcgggg ctcgagagcg ccagcaccga
      901 gtagaggaag gcgaagctca gcccgatcag aacgtagacg ttcactgccg cccacaaggt
      961 ctccgccgtc acctctttga cgtcgaacag cagttcgcgg gtcaccagcc aggacaggaa
     1021 cccaaagaag acgaagccga ccagtgcgga cgtgcccagg aaccaatcgc ctccgcccag
     1081 gacggaacca atccccatga caaacacgac gatggcgaac acgcccgtga tccagagggt
     1141 ggcgcgggtg cgggcgacgg ccagcggccc ggtaatgaac acgaacacca gcacgagctg
     1201 cgtagtcagg cgggcgccgg gttcgtcccc ggcgagtggc agcgcgaaca ggaacgcgag
     1261 cagcgacagg cagaggtatc tctgcttgcc aagggcctga acgattcgcc aggacgtaag
     1321 tggccgcatc tcggtcatcg tccgagccag cccgctccgc tcaaagggaa gctcaagatt
     1381 ccttcaatca cgccagcaat cacgccagca atcacgccag cgtctcgagc ttgccccgtg
     1441 cgcctcaagc cacctccgcg gtcggcatgc ccgacggaga agcgcgccga aatgctatgg
     1501 gcgaggtgcc ggcgaaacga cgaaatgcgc gggtaaacga tcctggatct gcgtaaccca
     1561 gctcgtagcc aatctgagtg acgggtagct ccggcgactc taggagcaat tcgcgcgcaa
     1621 cctcgaatct aacctggtcg accatgccgg aataggagat gcccacatcg gagaggcgtc
     1681 gctggagcgt tcgccgactc aagcctgcag ccaaggcaac gttgctgaaa ctcagctcgc
     1741 ccacgcgcac gtaactcctg atcagttccc gcatggattg aacgaagtcc agatcaccaa
     1801 agtcatccgg cagagcccgc tcgctcaaac cggcatcccg ggaactgctg ggtaatgcca
     1861 gcgcagaaag ggggatgtga atagttgcag aaatcggcac cgcgcgaacg gacacgcctt
     1921 cgaacagcgg caaggatgcc agggtcgatg gctccgccac cgatacgtcc acggacgggg
     1981 gcgaccaccc gtcccccaag ctcattcgta cgtgaccgat gaggttggcg acacagaaaa
     2041 tctcggtctc cgcaagcggc gatctgtcct gcttttccca atggccctcg ccctttgcga
     2101 ccgaaaaccg aaagcggttc tccgggctgt agcgcgtcac gttggcgttg tgcctgcgaa
     2161 tggcctcaag cagggtgacc gatttccagg ccgtgtcaga gaaggggttg ggctgaaagg
     2221 cagcgttgaa caaccctgcg gcgacgctca cggcaccggg cggtccatga ctcaccgctt
     2281 cgatgatgta cccgtgcagc aggcgcgccg agaccatgta gtcgggaatc agctcgaaat
     2341 ccgccacgat cccgtgtttc gccaacaggg gctccggcgc cacgccatgc tcccgtaacg
     2401 catgcatgta agcgatgaga tacgtgttcc tgagtagcca gattggttgc acttggtagg
     2461 accttgagta ccgctcgcgc cgcccggtac catcgcgaaa gtggcaccaa atggcaagtg
     2521 ggggccgcca ggatcgtcta ccgtcggcgg cctcgagtcg gtgagcaacc agattcgcga
     2581 cgctgatgca attcgcccgg ccctgttcga catcgaaggc gcgatcgcgg gctatttcga
     2641 atggaaaaag cagctctacg accgtcgggg attcgccacg agcaccactc ttttggccct
     2701 gtatcagcat gcagacaggt ccatctccgg caacaagcat ggggccggcc tgatattccg
     2761 ctggcaggga agctgggacc tggtcggccg ggaacgcaga cgacgggtgt cggagcgatc
     2821 ggcgctgtca tgaagggcat ggtcaccgat gagatctggc tgggcgaagg ggtttacgac
     2881 gccaacgcgt ccagcggcga gttcgactgg agctccgtcg gtgagggcga gtacctcaag
     2941 cacctcgagg tcggctgcac accggccttc gcccgtcgag ccaagaacac agtccagctc
     3001 acgtggtggg aaaaggatcg acggaaagac gccgggacgc cgtgcgtgac gaatgggtca
     3061 tcgagtccag ctacaaactt cagctcaccc gtaccgtgac gctgctaccc aacgtgcggg
     3121 tcctgatcgg ccccgcagcc gaccccggcg cagacacgac cgtggtgctc gggataaggg
     3181 caggcataac catctggcgc agcccacagc atccagcccg aacacagcaa cgcacagccg
     3241 ccaactccat cacggcaaac gtcatgg
//
LOCUS       DBNHQR010000136         3777 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_583240, whole genome shotgun sequence.
ACCESSION   DBNHQR010000136 DBNHQR010000000
VERSION     DBNHQR010000136.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_583240"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="AC16VR_03015"
     CDS             <1..698
                     /locus_tag="AC16VR_03015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6010155.1"
                     /translation="EPAVRASAGLLSESTGIIDSHAYMVSLQGDLESAGGVVAFNTEV
                     LDLGIERGLRVLTSTVTLECRRLVNAGGLWAPDLARRLDPSAPRAFYASGHYFTYSGP
                     APFERLVYPVAEPGGLGVHVTLDLAGQVKFGPDVTWLDGVDYTFDETRLAGFVESIRR
                     YFPAVEPARLQPGYTGIRPKIAGPGEPNADFRIDGPETHGIGGLVNLLGIESPGLTAS
                     LAIADLVAERLSA"
     gene            704..1825
                     /locus_tag="AC16VR_03020"
     CDS             704..1825
                     /locus_tag="AC16VR_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016493676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-NAD kinase family protein"
                     /protein_id="HIR6010156.1"
                     /translation="MGLLVNPVAGIGGAVALKGSDGADVQRAAAARGGEPRGEARARR
                     ALRAAGPATAQLRWLTWDGGMGADLLRSEAVICEVLGAPGDPPGPSDTRQAATAFAEA
                     GADLLVFAGGDGTARDILDAVGERLPVLGIPAGVKMHSGVFAITPEAAGEILVRLVEG
                     GLVRSCRADVRDLDETALRDGVVRPRYFGELLVPELGRFLQRTKESGRENEAMALGEI
                     VADVVERVRASDSSMSFVLGPGSTVAAIKDELGMKATLLGVDVYRDGAQIGEDVDAAW
                     LEQRLQSPFSLVVSFTRGQGFLLGRGNQQLSPAFLARLDRGNLIVPATRSKLVSLDGR
                     PLLIDTDDAELDRTFAGLVEITSGYEDRLLYRVESRAVV"
     gene            1812..2720
                     /locus_tag="AC16VR_03025"
     CDS             1812..2720
                     /locus_tag="AC16VR_03025"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6010157.1"
                     /translation="MRSFDPVIERCEQRYGGGSDAVRLFHGRGHAFEGYEDVTVDVFP
                     PYVLVGSFGDDDAGALRLADRLREALPGVAGIAVQLRAGRRTQVEVPHGRVPDRIVTS
                     EDGLRFIVRPLRNQNVGLFLDMAPARRWVRESALGGNVLNLFAYTCTFSVAALAGGAE
                     QVVNNDMSRAALDWGAENHTENEQDTRRVRMLPHNLFKSWWKIRQWAPYDLVIVDPPT
                     NQGGSFNAEKQYGQILKRLRELTRPGGRVLACLNSPFLNRDFLPAQMSRWCPECQFEG
                     YLANPDDFPDRYPDRALKVGVFTRSG"
     gene            complement(2712..3173)
                     /locus_tag="AC16VR_03030"
     CDS             complement(2712..3173)
                     /locus_tag="AC16VR_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010158.1"
                     /translation="MSDINIDDFFKDAAIALVQLYGMFPRRHALFVEDVCGPDETDEF
                     GMHSDRYLACFGTLLWLGEEGYVRYEQSIRQDAIDQAVLTARCFTVLATPAPRPTPPD
                     TDCNLPESIRVEQSTTINRLRAALKARSSPQLRAEMVELMTQMGTRLNVSS"
     gene            complement(3170..>3777)
                     /locus_tag="AC16VR_03035"
     CDS             complement(3170..>3777)
                     /locus_tag="AC16VR_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR6010159.1"
                     /translation="IFADEIYDKILYDDAEHVPLATLAEDLLVVTMNGLSKTYRVAGF
                     RSGWMVVSGAKHRAQDYMEGLNILASMRLCANVPAQHAIQTALGGYQSIQQYLVPGGR
                     LYEQRSVAWEMLNAIDGVSCVKPKGALYLFPKLDVRKFNITSDERFVLDLLRQERILV
                     VQGTGFNWFEPDHFRIVFLPHEEDLRHALERLEHFLAGYRQ"
BASE COUNT          598 a         1178 c         1306 g          695 t
ORIGIN      
        1 tcgaaccggc ggtgcgggcc agcgcggggc tgctttccga gtccacgggc atcatcgact
       61 cccatgccta catggtgagt ctgcaggggg acctggaatc ggcaggcggc gttgttgcct
      121 tcaatacgga agttctcgat ctcgggatag agcggggcct gagggtgctg acgagcaccg
      181 tgacgctcga gtgccgccgc ctggtgaatg caggtgggct ctgggcaccg gacctggccc
      241 gtcgtctgga tccttccgcg ccccgggcct tctacgcaag cggccattat tttacctaca
      301 gcggcccagc acccttcgag cgtctcgtct acccggttgc agagccgggc gggctgggcg
      361 tgcacgtcac gctggatctg gccggccagg tgaagttcgg gcccgacgtg acgtggctcg
      421 atggggtgga ctacaccttc gacgagacgc gcctggcagg cttcgttgaa tccatccgcc
      481 ggtatttccc ggcggttgag cccgcccgcc tccagcccgg ctacaccgga atacgcccga
      541 agatcgccgg gcccggagag cccaatgccg atttccgcat cgacggtccc gagacccacg
      601 gcattggcgg cctggtgaac cttctcggca tcgagtctcc gggtctgacg gcctccctgg
      661 ccatcgccga tctcgtcgcg gagaggctgt cggcatgatt tgcgtggggc tgcttgtcaa
      721 tcccgtcgcc ggcattggcg gtgccgtggc tctcaagggc agcgacggtg cggacgttca
      781 gcgggcggcg gctgcccggg gcggcgagcc tcgcggagaa gcgcgcgcac ggcgtgctct
      841 tcgcgcggcc gggccggcga ccgcgcagct gcgctggctg acctgggacg ggggcatggg
      901 cgcagatctg ctacggtcgg aggcggtgat ctgcgaggtg ctcggcgcgc cgggcgatcc
      961 gccagggccg tcggatactc gtcaggccgc aaccgccttc gccgaagccg gtgcggacct
     1021 gctggtcttc gccggcggag acgggaccgc gagagacatc ctcgatgccg tgggcgaacg
     1081 ccttcccgtt ctcggtatcc cggcaggggt caagatgcac tcgggcgtct ttgcgatcac
     1141 gccggaggcg gctggcgaga tactcgttcg tctcgtggag ggcggcctgg ttcggtcctg
     1201 ccgcgcggac gttcgcgatc tcgacgagac ggcgctgcga gacggcgtgg tgcgcccgag
     1261 atacttcggt gagcttctcg tgcccgagct cggccgtttt ctgcaacgca ccaaggagag
     1321 cggccgcgag aacgaggcga tggcgctggg cgagatcgtc gcggacgtgg tggagcgcgt
     1381 tcgcgcgtcg gattcatcca tgtccttcgt tctgggtccc ggcagcaccg tagcggccat
     1441 caaggatgag ctgggaatga aagccaccct gctcggcgtt gacgtctatc gggacggcgc
     1501 ccagatcggc gaggacgtcg acgccgcctg gctggagcag cgcctgcaat cgcccttttc
     1561 gctcgtggtc agctttacac gcgggcaggg gttcctgctg gggcgaggca atcagcagct
     1621 ttccccggcg tttctcgctc gcctggaccg cggcaacctg atcgtgccgg caacgaggag
     1681 caagcttgtg tcgctggatg gtcgccctct gctgatcgac acggacgacg cggagctcga
     1741 ccggaccttc gctgggctgg tagagatcac ctccggctac gaggatcgtc tgctgtaccg
     1801 ggtggagtca cgtgcggtcg tttgatccgg tcatcgagcg ctgtgagcag cgctacgggg
     1861 gtggcagcga tgcggtccgg ctctttcacg gccgggggca cgcgttcgaa ggctacgagg
     1921 atgtcaccgt ggacgtcttt cctccctacg ttctcgtcgg aagctttggc gacgacgacg
     1981 ccggcgccct gcgcctggcc gaccgtcttc gcgaagcgct gcccggggtc gccggaatcg
     2041 cggtgcagct cagggcaggg cggcggacgc aagtcgaggt tcctcatggt cgggtacccg
     2101 atcggatcgt gacgtccgaa gacgggctgc gcttcatcgt ccggccgctt cgtaaccaga
     2161 acgtggggct ctttctcgac atggcgccgg cgcggcggtg ggtgcgggag tctgcgttgg
     2221 gcggcaacgt tctcaacctg ttcgcctaca cctgcacgtt ctcggtcgcc gcccttgccg
     2281 gcggcgccga gcaggtcgtc aacaacgaca tgagccgcgc cgcgttggat tggggtgctg
     2341 aaaaccacac ggagaacgag caggacacgc gccgcgtgag gatgctgcct cacaacctgt
     2401 tcaagtcatg gtggaagatc cgtcagtggg cgccttacga tctggtgatc gtcgatccgc
     2461 cgaccaacca ggggggcagc ttcaatgcgg agaagcagta cggacagatc ctcaagcgtc
     2521 ttcgcgagct cacgcgcccg ggcgggcgcg tgctggcctg tctcaactcg ccctttctca
     2581 accgcgattt tcttcccgcc cagatgtccc ggtggtgtcc ggagtgccag ttcgaaggtt
     2641 atctagccaa ccctgacgac ttccccgacc gataccctga tcgagcgttg aaggtaggcg
     2701 tgttcactcg ttcaggatga cacgttcagg cgcgtgccca tctgcgtcat cagctccacc
     2761 atctccgcac ggagctgcgg cgatgagcgg gccttgagcg cggcgcgaag acgattgatg
     2821 gtggtcgact gctccacccg gatggactcc ggcagattgc aatccgtatc cggcggtgtg
     2881 ggtcggggtg cgggcgtggc gaggaccgtg aagcagcggg ccgtcaggac cgcctggtcg
     2941 atggcgtcct ggcggatgct ctgctcgtag cgaacgtagc cctcctcgcc gagccagagc
     3001 agggtgccga agcaggccag ataccggtcg ctgtgcatgc cgaactcatc ggtttcgtcc
     3061 ggaccgcaga cgtcctcgac gaacagggca tgacgccggg ggaacatgcc gtagagctgc
     3121 accagggcga tggcggcgtc cttgaaaaaa tcatcgatgt tgatgtcgct cactgccgat
     3181 agccggcaag gaagtgctcg aggcgctcca gcgcgtggcg caggtcctcc tcatggggaa
     3241 gaaacacgat gcggaaatgg tcgggctcga accagttgaa gccggtgccc tgcacgacga
     3301 ggatcctttc ctgacgcagc aggtcgagca caaagcgctc gtcactggtg atgttgaact
     3361 tgcggacgtc cagcttcggg aacaggtaaa gggccccctt gggcttgacg cagctcaccc
     3421 cgtcgatggc attcaacatc tcccatgcga cgctgcgctg ctcgtaaagg cgtccccctg
     3481 ggacgaggta ctgctggatg ctctgatagc cgcccagggc ggtctggatg gcgtgctggg
     3541 cggggacgtt cgcacacagg cgcatggatg ccaggatatt cagcccttcc atgtagtcct
     3601 gagcgcggtg cttggcaccg ctgacgacca tccagccgct gcggaacccg gcgacccgat
     3661 aggttttcga cagaccgttc atggtcacca cgagcaggtc ttctgccagc gtggccagcg
     3721 gaacgtgctc ggcatcgtcg tagaggatct tgtcgtagat ctcgtcggcg aagatga
//
LOCUS       DBNHQR010000137         1899 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_709812, whole genome shotgun sequence.
ACCESSION   DBNHQR010000137 DBNHQR010000000
VERSION     DBNHQR010000137.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_709812"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..633
                     /locus_tag="AC16VR_03040"
     CDS             88..633
                     /locus_tag="AC16VR_03040"
                     /inference="COORDINATES: protein motif:HMM:NF024911.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6010160.1"
                     /translation="MATEFEPGLVVCLTGPESTGKSTIAEQLASALAVPLVPEVAREY
                     LAGRPDYEQSDLLAIARAQLAAEQDALAEGTGLVVCDTDLQVIQIWWEERYAELDPWL
                     AQALVDRSARRYLLAVPDLPWEPDPLRESPHDRERLFRRYRQVLREGAFPYAEVRGLG
                     RSRFDSALVTVQRWLAEPQQD"
     gene            complement(635..1471)
                     /locus_tag="AC16VR_03045"
     CDS             complement(635..1471)
                     /locus_tag="AC16VR_03045"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR6010161.1"
                     /translation="MPGEDPDIGDIRRGICRLLPEWAPEELLDFDYLPGGYSNHNFRF
                     AHRASQYVVRVPLERPGAAALAGERSVYEALTDPSVPAVIALGDDGWLVSRWVEGPLL
                     VDAPPSAQTIVGFIRRLHESLRVRAAPYDPAARSRAYLETPGDSSPDARVMAMAHSLN
                     WRLDDTVACHNDLNPWNVIVSASGDWVTLDWQSVGANDPLFDLVTLHQGLSLPDAGLT
                     DLCEALLERPVAPERVERVLVGFWLREYAWAFAALQLGNRRQEILDQLALSRDRLASL
                     AG"
     gene            1532..>1899
                     /locus_tag="AC16VR_03050"
     CDS             1532..>1899
                     /locus_tag="AC16VR_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010162.1"
                     /translation="MEVTLSRFVHALRSAEVEVSPAETLDGFAVVRQVGIADPVLLQD
                     ALGLALAKTREEKARFADCFERLVRQLAFASGSGGEAAVRAAQGHRALRRPSAQRDRL
                     VDAGDVGHRGADIPVENQIC"
BASE COUNT          318 a          618 c          648 g          315 t
ORIGIN      
        1 cacggtcatc tatctggtca agggcattga gctgtatgct gttctctatg gcatttatct
       61 ggcaatggcc atcatgggct ggcgggcgtg gcgacggagt ttgaacctgg cctcgtcgtc
      121 tgcctgacgg gccccgagag caccggcaag agcaccatcg cggagcaatt ggccagcgcc
      181 ctggccgtcc cgctggtgcc ggaggtcgcg cgggagtatc tcgccggaag acccgactac
      241 gaacagtccg acctgctggc catcgcccgg gcgcagctcg ctgcggagca ggacgccctg
      301 gccgaaggca ccggtctcgt cgtgtgcgat accgacctgc aggtcatcca gatctggtgg
      361 gaggaacgct acgcggagct ggacccgtgg ctggcccagg cgctcgtcga ccgttcggcc
      421 cgtcgctacc tgctcgccgt tccggatcta ccctgggagc ccgaccccct gcgcgagtcg
      481 ccccacgacc gggagcggct ctttcgacgc taccggcaag tcctgaggga aggcgctttt
      541 ccgtacgccg aagtccgcgg tctggggcgg tcgcggttcg attccgcgct ggttaccgtg
      601 cagcgctggc tggccgaacc ccagcaggat tgaatcagcc ggccagagaa gccagccggt
      661 cacgggacag ggcgagctga tcgagaattt cctgacgccg gttaccaagc tgaagggcgg
      721 caaaggccca ggcgtactcg cgcagccaga atcccacgag cactcgctcc acccgctcgg
      781 gcgcgacggg ccgctccagc agcgcttcgc acaggtccgt cagcccggcg tccggcaggc
      841 tcaaaccctg atgcagggtc accagatcga acagcgggtc gttggcgccg acggactgcc
      901 agtcgagggt cacccagtca ccgctggccg agacgatgac gttccacggg ttgagatcgt
      961 tgtggcaggc gacggtgtcg tccaggcgcc aattaaggct gtgggccatg gccatcaccc
     1021 gggcgtccgg tgacgagtct ccgggcgttt cgagataggc acggctgcgc gccgcaggat
     1081 cgtaaggcgc tgccctcacg cggagcgatt cgtgcagccg cctgatgaaa ccgacgatgg
     1141 tctgcgctga cggcggggcg tcgacgagta aggggccttc gacccagcgg gataccagcc
     1201 agccgtcatc gcccagggcg atcacggccg gtaccgaagg gtctgtcagc gcctcgtaga
     1261 cggaacgttc gcccgccaag gccgccgcgc cgggccgttc gaggggaacc cgaaccacgt
     1321 actgagaggc ccggtgggcg aagcggaagt tgtgattgct gtatcccccg ggcaggtagt
     1381 cgaagtcgag caactcttcc ggcgcccact cggggaggag gcggcagatg ccgcgacgga
     1441 tgtcgccaat gtctggatct tcgcccggca agcgcaaggc ccctgaattc agccatcgga
     1501 tggtagcccg gcttgctaga atcggtcacc catggaggtc accctaagcc gcttcgttca
     1561 cgccctgcgc agcgccgagg tcgaggtttc gcccgcggag accctggacg ggttcgccgt
     1621 ggtgcgtcag gtgggtattg ccgatccggt tctgcttcag gacgccctgg ggctagccct
     1681 tgccaagacc cgcgaagaga aagcgcgctt tgccgactgc ttcgagcgct tggttcgcca
     1741 gctcgccttc gcctccggat ccggcggcga agcagccgtg cgcgcggctc aaggacatcg
     1801 agcgcttcgc cgaccgtctg ctcagcgtga ccggctagtt gatgccggtg acgtaggaca
     1861 tcgtggggcc gatatcccgg tggagaacca gatctgcga
//
LOCUS       DBNHQR010000138         2937 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1406609, whole genome shotgun sequence.
ACCESSION   DBNHQR010000138 DBNHQR010000000
VERSION     DBNHQR010000138.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1406609"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="AC16VR_03055"
     CDS             complement(<1..524)
                     /locus_tag="AC16VR_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HIR6010163.1"
                     /translation="MSVDQDLDELVLLFTADGGKVVSEIRYGEFEALIKKETSLAEHA
                     ASVVKAAYALVGGGLTLRGIVFFRFTVDEDGKIVGSFNLPLRYLLDNAGLGPDLGAGP
                     IQLACRGQCPVPWHSVNLWQPEGEGDGHPAMLAQKAIWRNRTGLKRTAAPPMAQPLRS
                     QPVEPPVIDEQRHL"
     gene            complement(943..1158)
                     /locus_tag="AC16VR_03060"
     CDS             complement(943..1158)
                     /locus_tag="AC16VR_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010164.1"
                     /translation="MYEVLRMALQDLVGKCFSVAEGEYRIVDVRNIAGDALVYAEKRQ
                     GLKEFMPSFPKRAAFHYGDIARYLAQS"
     gene            complement(1219..1575)
                     /locus_tag="AC16VR_03065"
     CDS             complement(1219..1575)
                     /locus_tag="AC16VR_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010165.1"
                     /translation="MAKAFCIWLSGLVVLTAASVGAAEEDAATGLAVAPGWDSVRTHC
                     GACHSYRLVTSQRGDEAFWRDIIRSMQGTQNLWPIAAEEEARIVGYLAANYAETDWGR
                     RPPLSPLLMPGDPTPD"
     gene            complement(1575..2852)
                     /locus_tag="AC16VR_03070"
     CDS             complement(1575..2852)
                     /locus_tag="AC16VR_03070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HIR6010166.1"
                     /translation="MERGLFELHEEPERADRLVWSRRRFIAGGVVATMERTLSAAIPY
                     GRFLPAGMIPVAIAESQPGTLVAEKSGLRVLNDRPLNAETPAHLLDDEITPAELLFVR
                     NNGVPPAAADPETWQLSVTGEACLAPRTFSLAELKREFRQYTYRLQLECGGNGRAEFR
                     PRVPGNQWTTGAIGCPEWTGVRLKDVLERVGVRGDAAYVAYRSADTHLSGDGSKDVIS
                     RGVPLSKALEAESLLAFQMNGEPIPHIHGAPLRMVCGGWPGSVSGKWVTEVLLRDRVH
                     DGAKMLGSSYRVPCRPVAPGESVSDEGMCIIQSMPVKSLITHPRSGSSAVVGRPMEVR
                     GHAWAGDRSIAEVAVSVDFGATWQPAVLARPANRLAWQRWRATVKLPVPGYYEIWARA
                     IDSAGVSQPMLVPGWNPKGYLNNAAHRIALRAG"
BASE COUNT          571 a          985 c          869 g          512 t
ORIGIN      
        1 tcgagatgcc gttgctcatc gatgaccggg ggttccacag gctggcttcg cagcggttgg
       61 gccatggggg gcgctgccgt acgtttcaga ccggtccggt ttctccagat cgccttctgg
      121 gcgagcatcg ccggatggcc gtctccttcc ccctccggct gccagagatt gacggagtgc
      181 catggaaccg gacactgccc ccgacaggcc agttgaatcg gccctgctcc gagatcggga
      241 ccgagcccgg cgttgtccag gagataacgc aacggcaggt tgaaagaccc cacgatcttg
      301 ccgtcctcat cgacggtgaa gcgaaagaag acgataccgc gcaacgtcag acccccgcct
      361 accagggcgt aggcagcctt caccacgctg gccgcatgct cggccaggct cgtctctttt
      421 ttgatgagag cctcgaattc tccatagcgg atctcggaga cgactttgcc gccgtctgcc
      481 gtgaacagga gaacgagttc atccaaatcc tgatctactg acatttccaa gcttgtcaac
      541 gcgcccggtt ggaacagccc atccctatcg aaagtggcca tccgcagacc agaagcactc
      601 ttccatgaag ctcgcacggg acacaaacac cgcgactcgc ctcattacag cagatggcca
      661 ttccccatac tgccaatggg atcacggctt tccgctgaat tcggcgggcg acgagcgcag
      721 cgaatatgcg ggtggcggcg aagaaacaga cggctacttg actagagctc gtaggcatcc
      781 acccagaccg attcgccgtc gtcagccgac gactcgtcga tccagtgatt ctcgagctcg
      841 ggctcgccga tctcttcaag ttccatgtga caaagacctc caggaaccca gatcctagac
      901 cgagtcgatg aacctaacgt gaaacgatcg cggctggcgg gctcagcttt gagcaagata
      961 tcttgcgata tcaccgtagt gaaatgccgc acgcttcggg aacgacggca tgaactcctt
     1021 gagcccctga cgcttctcgg catacacaag cgcgtccccc gcaatgttgc gaacgtccac
     1081 gatgcgatat tcgccttcgg ccaccgaaaa acactttcca accagatcct gaagagccat
     1141 gcgtaacacc tcatacacgc agagtttcag cataggcaaa ggtccccggt gcaggggtaa
     1201 ggggttgtaa tgggtgtttt agtccggcgt cggatcgccg ggcatgagca ggggcgaaag
     1261 cggcgggcgg cgaccccagt cggtctcggc atagttggcc gcgaggtatc cgacgatgcg
     1321 agcttcctct tcggcggcaa tgggccagag attctgggtc ccctgcatcg agcggatgat
     1381 gtcccgccag aaagcctcgt caccgcgctg tgaggtcacc aatcgatacg agtgacaggc
     1441 accgcagtga gtgcgcaccg aatcccaccc cggcgccacc gccaaccccg tcgccgcgtc
     1501 ctcttcggcg gctcccacgg atgcggctgt cagaaccacc agacccgaga gccagatgca
     1561 aaaggccttc gccatcaacc ggccctgagg gcgattcggt gggcggcgtt attgaggtac
     1621 cccttgggat tccatcctgg gacgagcatg ggctgggaca cgccggccga atcgatcgcg
     1681 cgggcccaga tctcgtagta gccgggcacg gggagcttta ccgtggcccg ccatcgctgc
     1741 cacgcgagcc ggtttgccgg gcgcgccaat accgcaggct gccaggtcgc accgaaatca
     1801 acgctcaccg caacctccgc tattgaccga tcgccggccc aggcatgccc gcgcacctcc
     1861 atcggccgac ccaccacagc gctgctgccg ctgcgggggt gggtaatcag cgatttcacc
     1921 ggcatggact ggatgatgca catgccctcg tcgctgacac tctcgccggg cgccacaggc
     1981 cgacagggaa cgcgatacga gctgccgagc atcttcgctc cgtcgtgcac ccgatcgcgc
     2041 aagagcacct cggtgaccca tttcccggat accgatcccg gccaaccccc gcagaccatg
     2101 cgtaacggcg cgccgtgaat atgcgggatg ggttcgccgt tcatctgaaa cgcaagcaac
     2161 gactccgctt ccagcgcctt gctcaacggg accccgcggg aaatcacgtc cttggaccca
     2221 tcgccggaca ggtgggtgtc ggcgctccga taagccacat acgcagcgtc accccgcacg
     2281 ccgacccgct cgagcacgtc cttgagccgc acgcccgtcc actccgggca accgatcgct
     2341 cccgtcgtcc attgattgcc ggggacgcgg ggtcgaaact ccgctctgcc gttgcccccg
     2401 cattcgagct gaaggcggta cgtgtattgc ctgaattccc gctttagttc cgccagggag
     2461 aaagtgcgtg gtgcaaggca tgcctctccg gtcacggaga gctgccaggt ttcaggatcc
     2521 gcggcggcgg ggggcacgcc gttgttgcgc acgaacagaa gctcggccgg cgtaatctcg
     2581 tcgtccagca ggtgcgcggg tgtctcggca tttagcggtc gatcgttgag aacgcgcaat
     2641 ccggacttct ccgcgaccaa cgtgccgggt tgggattccg caatcgccac gggaatcatg
     2701 ccggcaggca ggaagcggcc atagggaatg gccgccgaca gcgtcctctc cattgtcgcg
     2761 accacgccgc cggcaatgaa ccgacgcctg gaccagacca gccggtcggc gcgttccggt
     2821 tcctcgtgca gctcgaagag tccgcgctcc atcgggtctc accgctcgtt cagcgccctg
     2881 gaacgctgct caaccgaccc gttgcaggag cgtccccgtg ccgagcccgc cgccaca
//
LOCUS       DBNHQR010000139         3162 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_76685, whole genome shotgun sequence.
ACCESSION   DBNHQR010000139 DBNHQR010000000
VERSION     DBNHQR010000139.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_76685"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="AC16VR_03075"
     CDS             complement(<1..474)
                     /locus_tag="AC16VR_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010167.1"
                     /translation="MDDDTSKGPAGDDAIGEPAPEATESASRTSGEAADQAAKAASAK
                     PRGRLLGGLGLLLALGALCGVGYLYYLLVFLGPSDELYARLGRLEASLPEIRRDLAAA
                     RDAQAEEIESAVAAQQASLDETRRALLGALNEVTAAAAPAPREWKIAEVEYLLRIA"
     gene            complement(474..1175)
                     /locus_tag="AC16VR_03080"
     CDS             complement(474..1175)
                     /locus_tag="AC16VR_03080"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HIR6010168.1"
                     /translation="MRVWVTRTQPGADRQARRLRAAGHEPVVGPALRIVEVPGGAPRG
                     PFTDVVYLSEHAVRFGIARLTNQAWFSGARAHAVGAATAEHLAVAGIRAGFPEQASSE
                     GLLSLPEFADARGLTVLIVCGEGGRDLLETTLTARGARVEKLVVYRRRAVDEAPPDVG
                     SVDAIAVSSGDGFAAVARLWFAAGGRGDVPVLAPSPRVAGLGSALGFSNVYTCAGAGA
                     DALLKALESVSSTGR"
     gene            complement(1177..2094)
                     /gene="hemC"
                     /locus_tag="AC16VR_03085"
     CDS             complement(1177..2094)
                     /gene="hemC"
                     /locus_tag="AC16VR_03085"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HIR6010169.1"
                     /translation="MSALRLVIATRQSQLALWQANFVSARLREAHPGLTVELLGITTR
                     GDRWLSSPLAEVGGKGLFIKELEEALASGTADVAVHSVKDVPAELPEGFAMPVIAYRE
                     DPRDVLVSRDGVTLAGLHAGARVGSSSLRRQAQLLARRPDLLIEPIRGNVDTRLRKLD
                     DGDYDALVLAAAGLARLGLAGRASEYLATDDCLPAAGQGALGIECRAGDGRVRALLEP
                     LEDAQVAACVCAERTVSAVLGADCTAPLAAHATPLGQGVMLQSRLATTDGRKILEARG
                     EGADPVAVGKSVAESLLAQGAAEVLGGGD"
     gene            complement(2091..2819)
                     /locus_tag="AC16VR_03090"
     CDS             complement(2091..2819)
                     /locus_tag="AC16VR_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003157680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIR6010170.1"
                     /translation="MRVLIVDDEQLARDRLSRMVAELDDYEVAGEAGTGQDAVRLVAD
                     LQPEIILLDIRMPGMDGLEAARHLESVDEPPAVIFCTAYEEHAIEAFNLQAVGYLLKP
                     VRRENLRQALGNAQRVNKAQLAALSDDGSRRRSHISARTRKGIELVPLEAVRYFQADQ
                     KYVTVRHGEGELIIDETLRELEREFGELFIRVHRNALVAARHIIALDRLGDGHYQIRL
                     NDVDEHIDISRRHVASVRKFVRNL"
     gene            complement(2905..>3162)
                     /locus_tag="AC16VR_03095"
     CDS             complement(2905..>3162)
                     /locus_tag="AC16VR_03095"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6010171.1"
                     /translation="QPLPEGGTITVHLWLEGNDVNVRITNPIPEGEASNSSAGNRMAV
                     NNIRSRLMVLYGTRAELRAAPEEGLFVTSLKYPVSNMPLQT"
BASE COUNT          495 a         1128 c         1035 g          504 t
ORIGIN      
        1 ggcaatcctg agcaggtact ccacctcggc gatcttccat tcccgggggg cgggggccgc
       61 ggcggcggtg acctcgttga gcgccccgag gagcgctcta cgggtctcgt ccagtgacgc
      121 ctgctgagcg gcgacggcgc tctcgatctc ctcggcctgc gcgtcgcgcg ccgccgcgag
      181 atctcggcgg atctctggaa gagacgcctc caggcgcccg agccgggcat agagctcatc
      241 cgacggtccc aggaagacga ggaggtaata gagatagccg accccgcaaa gcgcgcccag
      301 ggcgagcagg aggccaagcc cgccgagaag gcggccgcgc ggttttgccg acgctgcctt
      361 cgcggcctgg tcagctgcct cccctgaggt cctggaagcg gattctgtcg cctccggggc
      421 gggctcaccg atggcgtcgt cgccggccgg acctttgctg gtatcgtcgt ccatcaccta
      481 cccgtcgaac tcacggactc gagcgccttt agcagggcat cggcgccggc tcccgcacat
      541 gtgtatacgt tactgaaccc caatgcggaa cccagcccgg ccacccgtgg ggacggcgcc
      601 aacaccggca cgtccccgcg accgcctgcg gcaaaccata accgcgccac cgccgcgaat
      661 ccatcgccgc tggagacggc gatggcgtcc accgagccga catccggagg ggcctcgtcc
      721 acggcgcggc gccggtagac gacgagcttt tccaccctgg cgccccttgc cgtcaacgtc
      781 gtctcgagca gatcccgccc tccttctccg cagacgatga gcaccgtcag gccccgggca
      841 tcggcaaatt ccggcagcga gagcagcccc tcggagctcg cctgctcagg gaagcccgca
      901 cgaatcccgg caacggccag gtgctcggcg gtcgctgctc ccacggcatg ggcgcgcgca
      961 ccggagaacc acgcctggtt ggtcaggcgc gcaatgccaa accgaacggc atgctcggaa
     1021 agatagacca cgtcggtaaa aggtccgcga ggcgcgccgc cgggcacctc cacgattctc
     1081 agcgccgggc ccacgaccgg ctcgtggccg gccgctctca gccttcgggc ctggcggtcg
     1141 gcgccgggtt gggttcgggt cacccagacg cgcaactcaa tctccgccgc cgagcacctc
     1201 ggccgcgccc tgcgccaaaa gcgactcggc gaccgatttg cccacggcta ccggatcggc
     1261 cccctcgcca cgggcttcca gaatcttccg gccgtcggtg gtcgccagac gggattgcag
     1321 catcacgcct tggccgagcg gcgtggcgtg tgcggccagc ggggccgtgc aatccgctcc
     1381 cagcacggcg ctcaccgtgc gctcggcgca aacgcaggca gcaacctgag catcttcgag
     1441 gggttcgagc agtgcccgca cgcgcccgtc gcccgcccgg cactcgatgc ccagcgctcc
     1501 ctggccggcg gcgggcaggc agtcgtcggt tgccaggtac tcgctggcgc gccccgccag
     1561 tcccagccgg gcgagacccg ctgcggccag caccagggcg tcgtaatcgc cgtcgtccag
     1621 cttgcgcagc cgggtgtcca cgttgccccg gatgggctcg atcagcagat cggggcgccg
     1681 ggccaggagc tgagcctgcc tgcgcaggct cgatgagccg acccgggcac cggcgtgcaa
     1741 cccggccaga gtcaccccat cccggctaac cagcacgtca cggggatcct cacgataggc
     1801 gatgaccggc atggcgaatc cttcgggaag ctccgccggc acgtccttta ccgagtgcac
     1861 ggcgacgtcg gccgtgcccg aggcaagcgc ttcttcgagc tccttgatga acaggccctt
     1921 gccgccgacc tcggcgaggg gcgaagaaag ccatcggtcc ccacgggtgg tgatgccgag
     1981 gagttccacg gtgaggcccg gatgggcctc gcgaagacgc gcgctgacga agttggcctg
     2041 ccacaaggca agctgactct gccgcgtggc tatgacgagc cgtaatgcgc tcacaggttc
     2101 ctgacaaact tgcgcacgga ggccacgtgg cgccggctga tgtcgatgtg ctcgtcgacg
     2161 tcgttgaggc ggatctggta gtggccgtcg cccagacgat cgagcgcgat gatgtggcgc
     2221 gcggccacca gggcgttgcg atggacgcgg atgaacagct cgccgaactc gcgctctagt
     2281 tcgcgaaggg tttcgtcgat gatcagctcg ccctcgccgt ggcgcacggt gacgtacttc
     2341 tgatcggcct gaaagtagcg cacggcttcg aggggtacga gctcgatgcc cttgcgcgtg
     2401 cgcgcggaga tatgggagcg gcgtcggctg ccgtcatccg agagcgctgc cagctgagct
     2461 ttgttgaccc gctgagcgtt gccgagcgct tggcgcaggt tctcccggcg cacgggtttc
     2521 agaaggtacc cgacggcctg gaggttgaac gcctcgatgg cgtgctcctc gtaggccgtg
     2581 cagaagatga cggccggggg ctcgtcgacc gactccagat gacgcgctgc ttccagcccg
     2641 tccatgcccg gcattcggat atcgagcagg atgatctccg gctgcaggtc agccaccagg
     2701 cgcacggcgt cctgcccggt gccagcctca ccggctacct cgtaatcgtc cagctcggcc
     2761 accattcgcg acaggcgatc tcgggccagc tgttcgtcgt ccacgatgag aaccctgatg
     2821 ctcatatccc ctccctgagc gtgctggtct ccaggctcgc cgccgcgctc gccgccacgc
     2881 ttgccgccgc gcttgccgcc gcgctcatgt ctgcaacggc atgttcgaca ccgggtactt
     2941 cagcgacgtg acgaacagcc cttcctcggg cgccgccctc aattccgcac gggtaccgta
     3001 caacaccatc agcctgctgc gaatgttgtt gactgccatt cggtttcccg cgctgctgtt
     3061 gctcgcctcg ccctccggta tcgggttggt gatgcgaacg ttgacgtcat tcccctccag
     3121 ccacagatga acggtgatcg tgccgccttc cggcagcggc tg
//
LOCUS       DBNHQR010000140         5506 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_837040, whole genome shotgun sequence.
ACCESSION   DBNHQR010000140 DBNHQR010000000
VERSION     DBNHQR010000140.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5506)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5506)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5506
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_837040"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="AC16VR_03100"
     CDS             <1..423
                     /locus_tag="AC16VR_03100"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HIR6010172.1"
                     /translation="LAWGLGRAEVEEIDALNILRASHLAMQRAVAALSVEPEMILVDG
                     NLLPVFSVPAVAVVKGDARVPEISAGAILAKVARDREMIELDRLYPGYGFAGHKGYPT
                     AEHLSALAELGACAVHRTSFAPVRDLKLRRPTVERLPL"
     gene            420..3923
                     /gene="dnaE"
                     /locus_tag="AC16VR_03105"
     CDS             420..3923
                     /gene="dnaE"
                     /locus_tag="AC16VR_03105"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR6010173.1"
                     /translation="MTAVTPASTEGFVHLRVHSEFSVADGLMKVGALADAARRLNMPA
                     VALTDRSNLFGLVKFYQACLSAGVKPLVGSDLVYQTDEGGRFRIAVLAMNSQGYRNLL
                     ALVSAAYLDTDERGVLSAGQIFARQEGLLVLSGGRQGEVGQALAKGDLPGAQRIAALW
                     EERFPERFYLELVRTGRGGEDAYIDAAVRLAAELELPVVATNDVCFLAADDFEAHETR
                     VCIYDGRTLDDPRRERSYSSDQYLRTPAEMTALFSDLPEAIENTVQIARRCNVQIELG
                     DYHLPNYPVPEGETLGGFLQRCAREGLERRWQTLAGRPEQAFSFEEYRERLSYELDVI
                     NQMGFAGYFLIVMEFIAWAREHGIPVGPGRGSGAGSLVAYALGITDLDPLGYDLLFER
                     FLNPERVSMPDFDIDFCMEGRDRVISHVSERYGHDAVSQIITFGTMAAKAVVRDVARV
                     QGKPYGLADKLSKLIPFEVGMTLEKAVSQSSELADFIASSEEVGEIMDMAYKLEGIVR
                     GLGRHAGGVVIAPSALTDFVPLYMDDQSGGLVSQFDKDDVESAGLVKFDFLGLKTLTI
                     IDWAVQAVNAARGDADELLDIDHIPLDDPGTFEFLRTAETTGVFQLESSGMKDLIRRL
                     KPDSIDDIIALVALFRPGPLQSGAVDDYIDRKHGQEPITYPHPEMKAVLQNTYGVMLY
                     QEQVMQTAQVLAGFSLGQADLLRRAMGKKKPEEMAKVREQFLSGCSERGVDAALAGQI
                     FDLMEKFSGYAFNKSHSATYALVSFQTAWLKTHHPAEFMAATLSVDMQNTDKVVTLVD
                     ETRNMGLPLSPPSVNRSEYRFSAREGAVVYGLGAVRGVGEGPVQALVEARASGGAFES
                     LPDFCRRVDSRKANRRVLEALIRAGAMDDFAEPGEALDCLRARLMAELGDALHGAEQA
                     ARDVALGMSDMFGGLEEPTLGNGNGRGAPPALGKRERLEWEKEALGLYLSGHPIEDYL
                     GEIREFCGRPIADLEAGKRTQVVAGLVFSARTMRGRRGEMGFVVLDDRSKRIEVSLFS
                     EVFQAGKEKVAKDAVLVVEGEVQPDDYTGELKMRADNVYTIEEARRRFARELVIDCCD
                     ASPTADFSSRLKTCLEPYRDGSQGCSIAVMYQSPATSGPGARGRIVLGRDWAVIPCDD
                     LLASLRSEFGDDRVAFRYAGN"
     gene            3928..5205
                     /gene="tilS"
                     /locus_tag="AC16VR_03110"
     CDS             3928..5205
                     /gene="tilS"
                     /locus_tag="AC16VR_03110"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIR6010174.1"
                     /translation="MDAGQLVEEALRRALRGAVAPGRVFVGYSGGMDSTVLLHAAIAA
                     SPVPVTAVHANHGLDPQAGRWQAHCERVCAQWGVGLESREVSVGHGNVEAAARAARYG
                     FFESVLSASDLLLLAHHQDDQAETVMLRMVQGRGLIGIPSARRLGAGELRRPFLELPR
                     QVLAEYADGLALDWVEDPGNADEGLDRNYLRGRILPSLRRRWPGFAGQMQVLLDQRAS
                     TEQMLLARVDGDFESIAVGELLCGDGGSGAELLGIWLSSLGAALPSRGALASFVAQLG
                     SAADRQPELKLAQGSLRRYRGRVYRVADPPALAPSYELIPPGELRLPHGTVTVCEAHG
                     GGFQLHGPLKVMFRGPEGAGHIRCRGHRRSIKKLLQDAGLPPWKRLTYPLLEDADGVA
                     AVPGIAERDPDPDPDPPSGGTRWVARWTAHSIR"
     gene            5287..>5506
                     /gene="pyrG"
                     /locus_tag="AC16VR_03115"
     CDS             5287..>5506
                     /gene="pyrG"
                     /locus_tag="AC16VR_03115"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252327.1"
                     /note="CTP synthase; cytidine triphosphate synthetase;
                     catalyzes the ATP-dependent amination of UTP to CTP with
                     either L-glutamine or ammonia as the source of nitrogen;
                     in Escherichia coli this enzyme forms a homotetramer;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthetase"
                     /protein_id="HIR6010175.1"
                     /translation="MTRFIFVTGGVVSSLGKGIASSSLAALLEARQLNVNVLKMDPYI
                     NVDPGTMSPFQHGEVFVTADGAETDLDLG"
BASE COUNT          896 a         1728 c         1889 g          993 t
ORIGIN      
        1 cttgcgtggg ggctcggacg cgcggaggtc gaggagatcg atgcgctcaa catactccgg
       61 gccagccatc ttgcgatgca gcgggcggta gcggctcttt ccgtggagcc ggagatgatc
      121 ctcgtcgatg gaaacctgct gccggtcttc tcggttcccg ccgtcgcggt ggtcaaaggg
      181 gacgcccgcg tcccggagat cagcgccgga gcaatcctgg ccaaggtggc gcgtgatcgg
      241 gaaatgatcg aactggatcg tctttatccg ggttacggct ttgccggcca caagggctat
      301 ccgaccgccg agcacctctc ggcgctcgcg gagctgggtg cgtgtgccgt ccaccggacg
      361 agctttgcgc cggtccgcga tctcaagctc cgtcggccga ccgtggagcg acttccgctg
      421 tgaccgccgt aaccccggca agcactgaag gcttcgtcca cctgcgggtt cacagcgagt
      481 tctcggtcgc cgatggcctg atgaaagtgg gcgccctggc tgatgccgcg cggcgcttga
      541 acatgccggc agtcgccctg accgaccgga gcaacctgtt cgggctggtg aagttctacc
      601 aggcctgtct cagcgcgggc gtgaagccgt tggtgggctc ggatctcgtc taccagacgg
      661 atgaaggcgg ccggttccgg atagccgttc tggccatgaa ctcccaggga tatcgcaact
      721 tgctggccct cgtttccgcc gcctacctcg ataccgacga acgcggcgtg ctcagcgccg
      781 gccagatatt cgcccggcag gaggggctgc tggtgctttc cggcgggcgc cagggtgagg
      841 tgggccaggc tcttgccaag ggcgacctcc ctggggctca aaggatagcg gccctgtggg
      901 aggagcgctt tcccgaacgc ttctacctgg agctcgtccg caccggtcgc ggcggcgagg
      961 atgcttacat tgatgcggcg gtgcggctgg cggcggaact cgagctgccc gtggtagcta
     1021 ccaatgacgt ctgctttctg gccgcggacg acttcgaggc tcacgagacg agggtctgca
     1081 tctacgatgg ccgaacgctg gacgatccgc gtcgtgaacg cagctactcg tccgaccagt
     1141 atctgcgaac gcccgcggag atgacggctc tcttcagcga cctgcctgaa gcgatcgaga
     1201 acaccgttca gattgcccgc cgctgcaacg ttcagatcga gctcggcgac tatcacctgc
     1261 ccaactaccc ggtcccggaa ggcgagacgc tggggggatt cctccagcgc tgcgcgcggg
     1321 aaggcctcga gcgccgatgg cagacattgg cgggtcgtcc cgagcaggcc ttttcgttcg
     1381 aggagtaccg ggagcgcctg agctacgagc tcgacgtgat caatcagatg ggttttgccg
     1441 gctacttcct gatcgtcatg gagttcattg cctgggcgcg cgagcacggc attcccgtag
     1501 ggcccgggcg cggctccggc gcaggctcgc tggtcgccta cgcgctgggc atcacggatc
     1561 tagaccccct gggttacgac cttctgtttg agcgattcct caacccggaa cgggtttcca
     1621 tgccggactt cgacatcgac ttctgcatgg agggccgtga ccgggtcatc agccacgtgt
     1681 ccgagcgcta cggccacgat gccgtcagcc agatcatcac cttcggaacc atggctgcta
     1741 aagcggtggt tcgggacgtc gcccgggttc agggcaagcc ctacggtctg gccgacaagc
     1801 tctccaagct gatcccgttc gaagtcggca tgacgctgga aaaggcagtg tcgcagagca
     1861 gcgagcttgc ggatttcatc gcttccagcg aggaagtggg tgagatcatg gacatggctt
     1921 acaagctgga aggaatcgtt cggggcctgg ggcgccacgc cggaggcgtg gtcatcgcgc
     1981 cgtcggcgct gacggacttc gtccccctgt acatggacga tcagagcggc gggttagttt
     2041 cgcagttcga caaggacgac gtggagagcg cgggcctggt caagttcgac ttcctcggcc
     2101 tgaagacgct gaccatcatc gactgggccg tgcaggcagt gaacgcggcg cggggagacg
     2161 cagacgagct gttggacatc gaccacatcc ctctggatga cccggggacc ttcgagttcc
     2221 tgcgtacggc cgaaaccacc ggggtgtttc agctggaatc ctccgggatg aaggatctga
     2281 tcaggcggct gaagccggac agcatcgacg acatcatcgc gctggtggcc ctgttccggc
     2341 ccggtccgct ccagtccggt gcggtggacg actacatcga tcgcaagcat ggtcaggaac
     2401 ccataacgta tccgcacccc gagatgaaag ccgtgctgca gaacacctac ggcgtgatgc
     2461 tctatcagga gcaggtcatg cagaccgccc aggtgctcgc cgggttcagc ctcggtcagg
     2521 ccgacctgtt gcggcgcgcc atggggaaga agaagcctga agagatggcc aaggtgcgcg
     2581 agcagtttct ctccggctgc agcgagcgtg gcgttgatgc cgctctcgcc ggccagatct
     2641 tcgaccttat ggagaagttc tccggttacg ccttcaacaa gtcgcacagc gcgacctacg
     2701 ccctcgtttc ctttcagacc gcgtggctga agacccacca tccggcggag ttcatggcgg
     2761 ccaccctgtc tgtggacatg cagaacaccg acaaggtggt gactctggtg gacgagacga
     2821 gaaacatggg gctgccgctc agcccgccga gcgtcaatcg cagcgagtac cggttctcgg
     2881 caagggaggg cgccgtagtt tacggtcttg gcgccgtgcg gggcgtgggc gagggccccg
     2941 tgcaggcact cgtcgaggcg cgcgcgtcgg gcggcgcgtt cgaaagcctt cccgacttct
     3001 gccgccgcgt cgactctcgc aaagccaatc ggcgggttct ggaagcgctg atccgcgcgg
     3061 gggccatgga cgactttgcc gagcccggag aagccttaga ctgcttgcgc gcgcgcctca
     3121 tggccgagct tggcgacgcg ttgcacgggg ccgagcaggc cgcgcgggat gtcgctctgg
     3181 gcatgtccga catgttcggc gggctggagg agcccaccct cggcaacggc aacggccgtg
     3241 gggcgccgcc agcgctcggc aagcgggagc gcctggagtg ggagaaggaa gcgctcggcc
     3301 tctacctcag cggccacccc atcgaagact accttgggga gatcagggag ttctgcggcc
     3361 gccccatcgc ggacctcgag gccggcaagc gcacccaggt cgtcgccgga ctggtttttt
     3421 ccgcgcgcac gatgcgcggc cgccggggcg agatgggttt cgtggttctg gacgatcgca
     3481 gcaagcggat cgaagtctcg ctgttctcgg aggtcttcca ggcgggcaag gagaaggtcg
     3541 ccaaggacgc cgtgctcgtc gtggaggggg aggttcagcc ggacgactac accggagagc
     3601 tcaaaatgcg ggccgacaac gtctacacga tcgaggaggc acggcggcgc ttcgcccgcg
     3661 agctcgtcat cgactgctgc gacgcgtccc cgacagccga tttctcctcc cgtctgaaga
     3721 cctgcctgga gccttatcgc gatggttccc agggatgctc catagcggtc atgtaccagt
     3781 cgccggcgac ctccgggccc ggggcccggg gccgcatcgt gcttggtcgc gactgggccg
     3841 tcatcccctg cgatgatctt cttgcgagcc tccgctcgga atttggcgac gatcgcgtgg
     3901 ctttcagata cgccgggaac tgatcccgtg gacgccgggc agctggtcga agaggcgctg
     3961 cgaagggcat tgcgcggtgc cgtcgcaccg ggccgagtct tcgtcggcta cagcggcggc
     4021 atggactcca cggttctgct gcacgcggcg atcgccgcgt ctccagtgcc cgtcaccgct
     4081 gtgcatgcca atcacggcct ggatccccag gccgggcgat ggcaggcaca ttgcgagcgg
     4141 gtctgcgcgc agtggggggt gggtcttgaa tcccgcgaag tgtcggtagg ccacggcaac
     4201 gtggaagccg ctgcccgggc cgcccgttac ggcttcttcg aatcggttct ctccgcatcc
     4261 gacttgctgc tgctggctca tcaccaggac gaccaggcgg agaccgtgat gctgcgaatg
     4321 gttcagggcc gggggctcat tggtatcccg tctgcccgtc ggctgggcgc tggcgagctg
     4381 cggcgaccat ttctggaact gccgcggcag gtgctggcgg agtatgccga cgggctcgcc
     4441 ctcgattggg tagaggatcc cggcaatgcc gacgaaggtc tggaccggaa ctatctgcgc
     4501 ggtcgcatcc tgccgagcct gcgccggcgc tggccggggt ttgccgggca gatgcaggtt
     4561 ctgctggatc agcgggcgag caccgagcag atgctgctgg caagggtgga cggcgacttc
     4621 gagtccattg ccgtcggcga gctcctttgc ggggacggcg gctccggggc ggagctgttg
     4681 ggcatctggc tgagctctct cggggccgcg ctcccctccc gaggcgccct cgcctcgttc
     4741 gtggcgcagc tcggaagcgc cgccgaccgg caaccggagc tcaagctggc gcaggggtct
     4801 ctgcgccgct accggggccg cgtctatcgg gtcgcggatc cgcccgctct ggcgccgagc
     4861 tacgagctga tcccgccggg ggagctgcgg ctgccccacg gcaccgtcac cgtgtgcgag
     4921 gcgcacggcg ggggctttca gttacacggc ccgctcaagg tgatgttccg cggccctgaa
     4981 ggagcaggac acatccgctg ccgcggccat cgccgctcca tcaagaaact gctccaggat
     5041 gcgggtcttc cgccctggaa aaggctcacc tatccgctgc tcgaggacgc agacggggta
     5101 gccgcggttc cgggcattgc ggagcgggac ccggacccgg acccggaccc gccgtccggg
     5161 gggacgcgct gggtcgcccg atggacggcg cattcgattc gttgaggttg ccgggggctt
     5221 ttgttatctt tcaaggccat cttgcgttcg cagcaatcgc ggacctcggg cttcaagatg
     5281 gaatagatga cacgattcat tttcgttact ggcggtgtcg tatcctctct gggtaaaggt
     5341 atcgcctcgt cgtccctcgc agcgctcctc gaggcaaggc aactcaacgt aaacgtgttg
     5401 aagatggatc cctacatcaa tgtggatccc gggacgatga gcccgtttca gcacggtgaa
     5461 gtcttcgtga ccgccgacgg ggcggaaacg gatctggatc tcggca
//
LOCUS       DBNHQR010000141         8671 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_963912, whole genome shotgun sequence.
ACCESSION   DBNHQR010000141 DBNHQR010000000
VERSION     DBNHQR010000141.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8671)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8671)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8671
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_963912"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="AC16VR_03120"
     CDS             <1..490
                     /locus_tag="AC16VR_03120"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6010176.1"
                     /translation="LTVKAAYDRMLEQGEGGRIIMTSSTSGLLGNFGQTNYGAAKAGI
                     AGFMRCLWLEGLKYGINVNVLVPTATSRLTEDILPEDIQDRFPPETVSPAIVWLCTDE
                     AKEVTGRQWLVAGNNVSLLSWQITPIADRDVSEGPWDVADIGQRILASKESWPPINPL
                     RG"
     gene            501..1703
                     /locus_tag="AC16VR_03125"
     CDS             501..1703
                     /locus_tag="AC16VR_03125"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6010177.1"
                     /translation="MSGPLEGFKVIDLSAVVSGPLTGALLADLGADVIKVERTVTGDI
                     QRNVGSRRNGFSGFFHVLNRGKRSIALNLTTREGCDIVHRLSADADVVLQNFRPGVVE
                     RLGIDYARLCADNPGLVYLSISGFGQSGPRAKDRAYDPIIQAYAGITDVQGSIHRDNP
                     DQPEQVNMLLLDKLTAWTGCQAIMAALLARSRTGKGQHVELSMLDTAISFIWSDVAAD
                     LILQGDDVDRRPPIRASGTMQTYADGHGFTMTLSQPEFEGLCRAYGLDHIAEDARFAS
                     LDLRMRHRAELREIISSEVDEVALELTVAQAEERLLRHEVPFARVRRLADLPEDEQIQ
                     HNQMFRELNHPVAGPLRDARPAPRFASTPAEPGGPAPSVGEHTRQILEEISMGAETEK
                     LFERGIVS"
     gene            1700..2803
                     /locus_tag="AC16VR_03130"
     CDS             1700..2803
                     /locus_tag="AC16VR_03130"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6010178.1"
                     /translation="MNFGFTEEQNMLRDQVARFMQEACPIPKVREIGASQTGFDAALW
                     QQAAQLGWLSLIIPEENGGLGLRWIDLTVVLEETARGLCPLPIASQAMTAAAILRCGS
                     DSQKSSWLPAMAAGEVVCTTALYDDPNWIHPDAVQLTATPVEGGVRLDGVKPFVPDAL
                     AADYLLLAYRGPDGLGLAALSRDQVTFTPQPQMDSTKPAGRVVMDAVTVGDEFLLPLS
                     EADLDYLTDVGAVAVTAEMVGAAEAALNMTSEYAKERIQFGKPIGQYQGVKHRLADIY
                     VDVESFKSLLYYAAWAVDEAPDELPRAISMAKGYASDAFARIGIDGIGLHGAIGFTAE
                     YDIQLYLKRSKWARPMYGDSDYHLDRVATLGGL"
     gene            2803..3963
                     /locus_tag="AC16VR_03135"
     CDS             2803..3963
                     /locus_tag="AC16VR_03135"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6010179.1"
                     /translation="MDFEYTADEQAFREEVRDFLATNLPPREERGKDFLANWLTNVRE
                     KGWVGFSWPKEFGGSDGGLVEQAILREEMAVAKAPPLGTSFMGLAWVGPGIIQYGTED
                     QKRRFIPDILDSKVIWCTGYSEPNHGSDLAAIQCKAVREGDHYVVNGQKIWTSGAPWS
                     QWIILLVRTDFDVDVKHKGITCLLVPMDSPGVEVKPIENMAGDRHFAEVFFTDVKVPL
                     ENRLGDEGDGWKVTVSALANERSSIGEVTQMFGKLDTLKELVRTTTRQGRPASEDPKV
                     RRTIAMFETKIAAMKYNGLRYLTKQIKGEPLSSETSVNKLHRASLEIEMDDFAVELTG
                     QAGLLLSGSEEAVSGGSWAKSSLSWPNVVIGGGTPNIQRNIIAERILGQPKD"
     gene            complement(3990..4589)
                     /locus_tag="AC16VR_03140"
     CDS             complement(3990..4589)
                     /locus_tag="AC16VR_03140"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR6010180.1"
                     /translation="MSVDVRIGLISDTHIPEARKTLWPQVFTAFDGVDMIMHAGDIHE
                     LYVLDALEEVAPVVAARGNGEDGGGGRPVQPDDPRVKYAWLLELGGLWVGLTHYVPVP
                     ERPPQFTLARWVERYFPERTPDIIVSGDTHREIIAEVQGVLCVNPGSPTYPHNYDTQY
                     GTLGFLDLVDGEARPSVWQITDGGIEPFDWDAVPPWKRR"
     gene            complement(4586..5854)
                     /locus_tag="AC16VR_03145"
     CDS             complement(4586..5854)
                     /locus_tag="AC16VR_03145"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6010181.1"
                     /translation="MSSRNIYQALEVPDFRWLWVGSLASSFAMNMQIVARGWLVYTLT
                     SSALDLAWVTLSFMVPMVAFSLWGGVVADRLPKRTIIAIAQALNTLATIVMGVIIFSD
                     LVSFWDFVWFGFFNGTVLALSMPARQAFAPELLPDRLIFTAMALNTTSWNLSRILGPA
                     FAGFLIAWVAAGDTSSTYGVGIVYVVIAALYFLSMATVLLVRKPGIVSSREQRKSPLT
                     DMADAFRYVWANPPVFGLIVLSIVPFLFGMPINTLLPAFNEDILGGGPDDLGLLMSTM
                     GMGAIVGSLLLAGVNDFRHRAAWLIATCVGWGAFTCAFGFSQTLMLSAGLLAVIGFLS
                     SWNMSLNRGLLQLQVDDHMRGRILSVDMMSHGLMPLGVIPISLIAEQFSVATALVVSG
                     AIFIVVVLLLLVVSPSIKRVDDDLQPVSGG"
     gene            complement(5957..6799)
                     /locus_tag="AC16VR_03150"
     CDS             complement(5957..6799)
                     /locus_tag="AC16VR_03150"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6010182.1"
                     /translation="MLKWQIGEVSVTQIVELSTASLGPYLLPQADPGALLEVPWLRPF
                     IDDDGRIVLSVHALVVESEGERLIVDTCIGNDKERTYPRWNRMQTDFLDRLAQAGYPP
                     ETIDTVMCTHMHVDHVGWNTRLTDDGWVPTFTRARYLFAEDEWAYWQREPQEFGPVVE
                     DSVQPIFDAGLAQVVGQTHRVTDEVWLEPTPGHTPGHVSVHIASAGEEAIITGDMIHH
                     PCQIAHPEWSSSADWNQEASAETRDAFLERYADRPVLVIGTHFAAPTAGHIVRDGESF
                     RLDY"
     gene            6940..7725
                     /locus_tag="AC16VR_03155"
     CDS             6940..7725
                     /locus_tag="AC16VR_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015477185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6010183.1"
                     /translation="MTRFGDIAVETTGHVAVLEIQRPPNNFFDVDLINAMGDAFEAMD
                     EDANVRALVLAADGKHFCAGANFGNRDEQSRRQARTATDGNPLYSAAVRLFACRKPVI
                     AAVQGAAVGGGFGLAVMPDFRVVCPETRFTANFVKLGFHPGFGLTYTLPRLIGQQRAN
                     LMFMTGRRIDGETACEWGLADILTDRDSLRARAMELAQEIAENAPLAVTSVRATLREG
                     VAEAVKRQTDHEFIEQHRLQQTEDHKEGVRAVAERRPGNFKGR"
     gene            complement(7712..>8671)
                     /locus_tag="AC16VR_03160"
     CDS             complement(7712..>8671)
                     /locus_tag="AC16VR_03160"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6010184.1"
                     /translation="HERSRLFGVRHGCFTFGSILSLVSSYFLISAEQQGAEEVRNTAF
                     WLAVLAGGVMCLLMAFGVWKLRERADYQGRVNENPIQAFADVWRNRHARIIIAVSFIE
                     NVGSAAIGALTLYVAQYVVGAPMWAPAIILCYMIPSTFSVPLWIPLSRRFGKIRLWIF
                     SLVLTGVAFGSMFVLPFMESMPLKLAYIATAAVFAGFAAGCGGTVSPSIQGDVIDYDE
                     YLTGERKEGSYFAAWNFVYKSATGIMLLLTGFVLQASGFVPNQEQTMTVQLAMITLYS
                     LFPLVCYSIGSLLLKRFDLDEDAHGEIRRALDERNAGRAASAP"
BASE COUNT         1680 a         2822 c         2811 g         1358 t
ORIGIN      
        1 cctcaccgtc aaagcagcct atgaccggat gctcgaacag ggagagggcg gccggatcat
       61 catgaccagc tccacctcgg ggctgctggg caacttcggc cagaccaact acggcgcggc
      121 caaagccggc attgcgggat tcatgcgctg cctctggctc gaggggctga agtacggcat
      181 caacgtcaac gtgcttgtac ccaccgcaac gtcgcggctc accgaagaca tcctccccga
      241 ggacatccag gaccgattcc cgccggagac cgtatcgccg gccatcgtct ggctgtgcac
      301 ggacgaggcc aaggaggtca ccggccggca gtggctggtc gccggcaaca acgtctcgct
      361 gctctcctgg cagatcaccc ccatcgccga ccgggacgtc tcggaagggc cgtgggacgt
      421 ggcggacatc gggcagagga tcctggcgag caaggagagc tggccgccga tcaatcctct
      481 ccggggctga ggcaggctcc atgtccggtc ccctcgaagg cttcaaggtc atcgatctgt
      541 ccgcggtggt ctccgggccg ctgaccggag ccctgctcgc cgacctgggt gccgatgtca
      601 tcaaggtcga gcggacggtc accggcgaca tccagcgcaa cgtgggcagt cgccggaacg
      661 gtttttccgg attctttcac gtgctcaacc gaggcaagcg gtccatcgcg ctgaatctca
      721 ccacccggga gggctgcgac atcgtccacc gcctctccgc ggacgccgac gtggttctgc
      781 agaacttcag gcccggtgtg gtggagcgtc tgggcatcga ttacgcgagg ctttgtgctg
      841 acaatccggg gctcgtctac ctttccatca gcgggttcgg ccagtccggg ccccgggcca
      901 aagatcgggc ctacgacccc attatccagg cctacgccgg catcaccgac gtgcagggga
      961 gcattcatcg ggacaatccc gaccagcccg agcaggtgaa catgctgctg ctggacaagc
     1021 tgacggcctg gaccgggtgc caggcgatca tggccgctct gctggcacgc agccgcacgg
     1081 gcaagggcca gcacgtggag ctgtccatgc tggacaccgc catctcgttc atctggtccg
     1141 acgtggccgc cgacctgatt ctgcaggggg acgacgtcga caggcgcccg cccatccgcg
     1201 cttccggcac catgcagacc tacgccgacg gccacggctt cacgatgacg ctgagccagc
     1261 cggagttcga gggcctgtgc cgtgcctacg gcctggacca catcgccgag gacgcccgct
     1321 tcgccagcct ggacctgcgc atgcggcacc gcgccgagct gcgcgagatc atcagctccg
     1381 aggtcgacga ggtggcgctg gagctcacgg tggcgcaggc ggaggagagg ctgctgcgtc
     1441 acgaggttcc tttcgcccgg gtgcggcgcc tggccgacct gcccgaagac gagcagatcc
     1501 agcacaacca gatgttccgg gagctgaatc accccgtggc gggaccattg cgcgacgcga
     1561 ggcccgcccc gcgattcgcc agcaccccgg cggagcccgg cgggcccgcg ccgagcgtgg
     1621 gagagcacac gcgacagatt ctggaggaaa tcagcatggg cgccgagacc gagaaactgt
     1681 tcgagcgagg tatcgtttca tgaattttgg cttcaccgag gagcagaaca tgctgcgcga
     1741 ccaggtcgcg cgcttcatgc aggaggcctg ccccattccc aaggtgcgcg agatcggtgc
     1801 ttcacagacc ggcttcgatg cggctctttg gcaacaggcg gcgcagctcg gctggctgag
     1861 cctcatcatc cccgaggaga acggcggcct tggactgcgc tggatcgatc tgaccgtggt
     1921 gctggaggaa acggcccggg gactttgccc gctgcccatc gcctcccagg caatgaccgc
     1981 cgccgccatc ctcagatgtg gcagcgactc gcagaaatcg agctggctgc cagccatggc
     2041 ggccggggaa gtcgtttgca ccacggcgct gtacgatgat ccgaactgga ttcatcccga
     2101 cgccgtgcag ctcaccgcaa cgcccgtgga gggcggcgtg cgcctggacg gcgtcaagcc
     2161 tttcgtgccc gacgctctcg ccgccgacta cctgctgctg gcctaccgag ggcccgacgg
     2221 gctggggctg gcagcactca gccgcgacca ggtcaccttt accccgcagc cgcagatgga
     2281 cagcaccaag ccggccggac gtgtggtcat ggacgcggtc accgtcggcg atgagtttct
     2341 gctgccgctg agcgaggcgg atctcgacta cctcacggac gtcggcgccg tcgccgtgac
     2401 ggcggagatg gtgggtgccg ccgaggccgc cctgaacatg accagcgagt acgccaagga
     2461 gcgcatccag ttcggcaagc caatcgggca gtatcagggc gtcaagcacc gactggcgga
     2521 catatacgtc gacgtcgagt cgttcaagtc gctgctgtac tacgccgcct gggccgtcga
     2581 cgaagcgccc gacgagttgc cccgggccat ctccatggca aagggttacg cctccgacgc
     2641 ctttgccaga atcggcattg acggcatcgg cctgcacggc gccatcggct tcaccgccga
     2701 gtacgacatt cagctttacc tcaaacggtc gaagtgggcg cgccccatgt acggggactc
     2761 cgactatcac ctggatcggg tcgcaaccct gggaggtttg tagtggattt cgagtacacg
     2821 gccgacgagc aggcgttcag ggaagaggtt cgcgattttc tggcgaccaa cctgccgccc
     2881 cgggaagaac gcggcaagga cttcctcgcc aactggctga cgaacgtcag agagaaggga
     2941 tgggtcgggt tttcctggcc gaaggagttc ggcgggagcg acggcgggct ggtggaacag
     3001 gccatcctcc gggaagagat ggccgtggcc aaggccccgc cgctcggcac ctcgttcatg
     3061 ggtctggcct gggtgggccc cggcatcatc cagtacggga cggaggatca gaaacgccgc
     3121 ttcatccccg acatcctgga cagcaaggtc atctggtgca cgggctactc cgagccgaac
     3181 cacgggtccg accttgccgc cattcagtgc aaggccgtgc gtgagggcga ccactacgtc
     3241 gtcaacggtc agaagatctg gaccagcggc gcgccgtggt cccaatggat catcctcctg
     3301 gtacggacgg acttcgacgt ggacgtgaag cacaagggca tcacgtgcct gctggtaccg
     3361 atggattctc caggcgtcga ggtcaagccc atcgagaaca tggccggtga ccggcatttc
     3421 gccgaggtct tctttaccga cgtgaaggtt ccgctggaga accgtcttgg cgacgaggga
     3481 gacggctgga aggtcaccgt gtccgccctt gccaatgagc gctccagcat cggcgaggtc
     3541 acccagatgt tcggcaagct cgacacgctc aaagaactcg tcaggaccac taccaggcag
     3601 ggacggcccg caagcgagga tcccaaggtc aggcggacga tcgcgatgtt cgaaaccaag
     3661 atcgccgcga tgaagtacaa cggtttgcgt tacctgacca agcagatcaa gggcgagccg
     3721 ttgtccagcg agacatcggt gaacaagctg catcgcgcaa gcctcgagat cgagatggac
     3781 gacttcgcgg tggagctcac cggtcaggcc gggctcctgc tctccggcag cgaggaagcc
     3841 gtgtcaggag gaagctgggc aaagtcgagc ctttcctggc cgaacgtggt gatcggcggc
     3901 ggcacgccca atatccagcg caacatcatc gccgagcgca tcctcgggca gcccaaggac
     3961 tgacctagcc gggccaacgg gcccgccggt cagcgacgct tccagggcgg cacggcgtcc
     4021 cagtcgaagg gctcgatgcc gccgtcggtg atctgccaga cgctgggcct tgcctccccg
     4081 tcgacgaggt cgagaaaccc cagcgtgccg tactgggtat cgtagttgtg gggataggtg
     4141 ggcgagcccg gattcacgca gagcacgccc tgcacctccg cgatgatctc ccggtgggtg
     4201 tcccccgaga cgatgatatc gggcgtccgc tcgggaaagt accgttcaac ccacctcgcc
     4261 agagtgaact gtggcgggcg ttcgggcacc ggcacgtaat gagtcaggcc cacccagaga
     4321 cccccgagtt ccagcagcca ggcatacttc acgcgcggat catccggctg cacgggtcga
     4381 ccgccgccac catcctcccc gttgccccgg gcggcaacca ccggggctac ctcctccagg
     4441 gcatccagga cgtacagctc gtgaatgtcc cccgcgtgca tgatcatgtc gacgccgtcg
     4501 aaggcggtga agacctgcgg ccagagggtt ttgcgcgcct ccggaatgtg tgtgtctgat
     4561 atcaggccga tgcgcacgtc gacgctcacc cgcccgagac gggctgcagg tcatcgtcca
     4621 cccgcttgat cgaaggggac accaccagaa gcaacagcac caccacgatg aagatcgctc
     4681 cgctgaccac cagcgccgtg gccacgctga actgttcggc aatgaggctg atgggaatga
     4741 cgcccagcgg catgagcccg tgggacatca tgtcgacgct gagtatccgc ccccgcatgt
     4801 ggtcgtccac ctgaagctgg agcagcccgc ggttgagcga catgttccag gagctcagaa
     4861 agccgatcac cgcgagcagc ccggcggaga gcatcagggt ctgggagaag ccgaacgcgc
     4921 aggtgaaggc cccccagcct acgcaggtag caatgagcca ggccgcgcgg tgccggaagt
     4981 cgttcacgcc ggccagcagc agggatccga cgatggcgcc catgcccatg gtcgacatca
     5041 gcagccccag atcgtccggc cccccgccca gaatgtcctc gttgaacgcc ggcagcaggg
     5101 tgttgatggg catgccgaac agaaacggca cgatggagag aacgatcagg ccgaataccg
     5161 gcgggttggc ccagacgtaa cgaaaggcgt ccgccatatc ggtgagcggg gacttgcgct
     5221 gctcgcggga actcacgatg ccgggcttgc gtaccagcag gacggtcgcc atggaaagga
     5281 agtagagcgc ggcaatcacg acgtagacga tgcctacccc gtaggtggag gacgtgtcgc
     5341 ccgccgcgac ccacgcgatg agaaagcctg caaacgcggg gccgagaatg cgtgagaggt
     5401 tccagctggt ggtgttcagc gccatggccg tgaagatcag cctatccggc aggagctcgg
     5461 gcgcgaacgc ctggcgggcg ggcatggaaa gcgcgagaac ggttccattg aagaaaccga
     5521 accagacgaa atcccagaaa gacaccagat cggagaagat gatcacaccc atgacgatgg
     5581 tcgccagggt gttcaacgcc tgcgcgatgg cgatgatggt gcgcttcggg agccggtcgg
     5641 ccaccacgcc accccacagc gagaaggcca ccatgggcac catgaacgac agggtcaccc
     5701 aggccaggtc aagagccgac gacgtcaacg tgtagaccag ccagccccgg gcgacgatct
     5761 gcatgttcat ggcgaaagac gaagccaggc tgcccaccca aagccagcgg aagtcgggca
     5821 cttccagagc ttggtagatg tttcgtgacg acaacggcgg atccggtagg gtcgggcaag
     5881 cccgtcactt tagcacagcc gcggggcgtc accgtcccag cccgaacccc gcgggagggc
     5941 cggccgggca ataaaatcag tagtcgagcc ggaagctttc cccgtcacgc acgatgtgcc
     6001 cggcagtcgg cgcggcaaaa tgggtcccga tcacgagcac gggacggtcg gcatatcgct
     6061 cgagaaacgc atcgcgcgtc tcggcgctgg cctcctgatt ccagtccgcg ctgctcgacc
     6121 attccggatg agcgatctgg cagggatgat ggatcatgtc ccccgtgatg atggcttcct
     6181 ctccggcgga agcaatgtgc acgctcacgt gaccgggcgt gtgcccgggc gtcggctcga
     6241 gccagacctc gtccgtgacc cgatgggtct gccccaccac ctgcgcgagc ccggcatcga
     6301 agatcggctg caccgagtcc tccaccacgg ggccgaactc ctgcggctcg cgctgccagt
     6361 aggcccactc gtcctcggcg aagagatagc gcgcccgggt gaaagtgggt acccacccat
     6421 cgtccgtcag ccgcgtgttc cagccaacgt gatcgacgtg catgtgcgtg cacatcaccg
     6481 tgtctatggt ctccggggga tacccggcct gagcgagccg atccagaaaa tcggtctgca
     6541 tgcggttcca gcgcgggtag gtgcgctcct tgtcgttgcc tatgcaggta tcgacgatca
     6601 gccgctcccc ttcggactcc accacgaggg catggacgct caacactatc cggccgtcgt
     6661 cgtcgatgaa cggccgaagc cagggtacct cgagaagcgc acccggatcg gcctggggca
     6721 gcaggtacgg gccgagcgaa gctgtggaga gctccacgat ctgcgtgacc gaaacctcac
     6781 caatctgcca tttcagcatg gaatccccct gacctcaccc cgacccggcg agccttgcag
     6841 atcggctgac ggcggtcaaa tcccgccgtt ccccggcctt gggcgcggca tgccatgtca
     6901 taatgtcgct cgtcagctaa ataccccggg ggagagatca tgaccagatt cggcgacatc
     6961 gcggtggaga ccaccggaca cgtggcggtg ctggaaatac agcgtccgcc caacaacttc
     7021 ttcgacgtgg acctgatcaa cgccatgggg gatgccttcg aggccatgga cgaggatgcc
     7081 aacgtccggg cgctggtcct ggccgccgac ggcaagcact tctgcgcggg cgccaacttc
     7141 ggcaaccggg acgagcagtc ccgccgccag gcgcgcacgg ccaccgacgg caacccgctc
     7201 tactcggctg ccgttcggct gttcgcctgc aggaagccgg tgatcgccgc ggtgcaggga
     7261 gccgccgtcg ggggcggttt cggccttgcc gtcatgccgg actttcgggt cgtctgtccc
     7321 gagacccggt tcaccgccaa cttcgtgaag ctcggcttcc acccgggctt cggccttacc
     7381 tacaccctgc cccggctcat cggccagcag cgcgccaatc tgatgttcat gacgggccgg
     7441 cgcatcgacg gcgaaaccgc ctgcgagtgg ggcctggccg acattctgac ggatcgggac
     7501 tccctccgtg cccgagccat ggagctcgct caggaaattg cggagaacgc gccgctcgcg
     7561 gttacctcgg ttcgggcaac cctgcgtgag ggcgtcgccg aagccgtgaa gcgccagacc
     7621 gaccacgagt tcatcgagca gcatcgcctg cagcagaccg aggaccacaa ggaaggcgtg
     7681 cgcgccgtgg ccgaacgacg gccgggcaat ttcaaggggc gctagcggcc ctgccggcgt
     7741 tccgctcgtc cagggcgcga cggatctctc cgtgggcgtc ctcgtcgaga tcgaagcgtt
     7801 tgagaagcag cgagccaatg ctgtagcaga ccagcggaaa caggctgtac agcgtgatca
     7861 tggccagctg aaccgtcatg gtctgttcct gattgggcac gaatcccgat gcctgcagca
     7921 cgaatcccgt gagcagaagc atgatgcccg tcgcgctctt gtacacgaaa ttccaggccg
     7981 caaagtacga gccctccttt cgttctcccg tcagatactc gtcatagtcg atgacgtcac
     8041 cctggatgga cggcgacacc gtcccaccgc agccggcagc gaagccggca aagacggcgg
     8101 cggtggcgat gtaggccagc ttgaggggca tgctctccat gaacgggagc acgaacatgc
     8161 tgccaaacgc gacaccggtg agcaccagcg agaagatcca gagcctgatc ttgccgaacc
     8221 gtctcgaaag cggaatccaa aggggcaccg agaaggtgga aggaatcatg tagcagagga
     8281 tgatggcagg ggcccacatg ggcgcgccga ccacgtactg ggcgacgtaa agtgtcagcg
     8341 ctccgatggc cgcggagccg acgttctcga tgaaagacac ggcaatgatg atgcgggcat
     8401 gccggttgcg ccaaacgtcg gcaaatgcct gaatcgggtt ctcgttgacc cttccctggt
     8461 agtcggcgcg ctccctcagc ttccagacac cgaaggccat gagcaggcac ataacgccgc
     8521 cggccagaac cgccagccag aaggccgtgt ttctgacctc ctcggctccc tgctgctcag
     8581 cgctgatcag aaagtaggag ctgacgagag agagaatcga gccgaaggtg aagcagccgt
     8641 ggcgaacgcc gaacagtcgg ctccgctcgt g
//
LOCUS       DBNHQR010000142         4416 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1090410, whole genome shotgun sequence.
ACCESSION   DBNHQR010000142 DBNHQR010000000
VERSION     DBNHQR010000142.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4416)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1090410"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="AC16VR_03165"
     CDS             complement(<1..486)
                     /locus_tag="AC16VR_03165"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6010185.1"
                     /translation="MQPVRKDNIRLAATILLVRDVGGGPEVFMVQRPGGVDFPDLHVF
                     PGGKVDAADFVPELVTDLDDRSASQRLGVEAGGLRYWVAAIRECFEECGVLLADRDGE
                     LFTPRTDAEAERFAGHRHALISGELDFAAMCRQEGLAIRAGRAHYFSHWITPEVAPRR
                     FD"
     gene            575..871
                     /locus_tag="AC16VR_03170"
     CDS             575..871
                     /locus_tag="AC16VR_03170"
                     /inference="COORDINATES: protein motif:HMM:NF015807.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HIR6010186.1"
                     /translation="MKEKYYKVLVRLPPEMREMLAESARHYRRSRNSDIVARLQESFR
                     GIADSARGRGLAPPMHDQFEQLFRADLSPQEQSLVTAFRRLSSDQQTALLALLN"
     gene            895..1887
                     /locus_tag="AC16VR_03175"
     CDS             895..1887
                     /locus_tag="AC16VR_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6010187.1"
                     /translation="MSLSPYHHPTTICLVDDNQSFLDSMILEMPSMWACRAFTDPHAA
                     AEFLNQPIGLPPLAERCLSRETSTGSDAVIHIDLGLIEQEVNHPDRFRRISVLVADYA
                     MPSMNGLELCAAARDPHLRKAMLTGVADEKLAVEAFNEGLIHRFIPKHGTSATAIARE
                     YIAELQAEYFEQYTALLQGSLTVGKPGFLTDPDVSQWVEALMRTERLVEYYLVDDPPG
                     LLMLGSDGAMFRAMILDPVELSHQLEIARRYGAPDHIVKGIAERRLAVLLAGDSPADY
                     YGAESFPWEENVCAVSPVGDDGKWFVGVVRDAPVDIDFDPRLSSYDAYLATLER"
     gene            complement(1967..3388)
                     /gene="sbcB"
                     /locus_tag="AC16VR_03180"
     CDS             complement(1967..3388)
                     /gene="sbcB"
                     /locus_tag="AC16VR_03180"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF008746.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HIR6010188.1"
                     /translation="MAASYYWYDLETSGIEPRWDRVIQFAGLRTDLSLNPVGDEYCTY
                     VELPDDVLPSPEATLVTGITPDVTRREGVSEWQAMTRINELFSAPQTCVAGYNSLRFD
                     DEFIRFGFYRMLIDPYAREWQNGNSRWDIIDLVRATGALRRDGIRWPTDDEGLPVYRL
                     ELLTKANDLEHGQAHDAMSDVRATVALARLIRDCQPKLFDYYFNGRSKKQVKQLLEPF
                     GARVCLHVSGMYPRSRYCAAPVVSLCRHPKNSNSIIVADLSEDVEPLIGWTDEQIRAE
                     LFKEGSTSRPPLKEIRINRCPFVAGIEVMTGENWERIGFDRREIKERQRRLARPGLAQ
                     KIMRVYGDRPPPAAPDVEAALYDAFLQDADRGRCASLQEALGQGKWLDLDYSDRRLVS
                     LSTRLKSRSFAESLSEEERQDWHEFVRDKLNAEDAPWLTLKEFDARLDRIRQDSAGED
                     ARRERLLGKLEAYAGGLKARYGG"
     gene            3413..>4416
                     /locus_tag="AC16VR_03185"
     CDS             3413..>4416
                     /locus_tag="AC16VR_03185"
                     /inference="COORDINATES: protein motif:HMM:NF013132.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR6010189.1"
                     /translation="MSASNAKRIALPAGPILALFAGYLMYSTGTTPAAAITLGITLIC
                     VVWWVFEPIPIPATSVLPLAMLPLLGVLTPQQVAQAYGHPLILLMLGGFILSTALARN
                     GAHRRIALTMVRAFGGSSSRRLVFGFMAASAVLSMWISNTATTLMLLPVALAVLEKAE
                     DPKLATPLLLGIAYAASIGGIGTPIGTPPNVVFMGVYNDLGVGQIGFADWMSFAMPVV
                     LVLLPIAAVWLTRRLGHQGTVDVPEVGAWQAGEVRVLMVFALTAVAWITRSEPFGGWT
                     ALLGLAQTNDAMVALLAVVIMFVVPDGKGERLLDWETATQIPWGMLILFGAGLTIAAA
                     "
BASE COUNT          740 a         1488 c         1407 g          781 t
ORIGIN      
        1 gtcgaagcgc cgcggcgcca cctcaggcgt gatccagtga ctgaagtagt gcgcgcggcc
       61 ggcccggatg gcgagccctt cctgccggca catggccgcg aaatccagct cgccggagat
      121 gagcgcgtga cggtgtccgg caaagcgctc cgcttcggca tccgttcgcg gggtgaagag
      181 ctcaccgtcg cgatccgcca gaagcacgcc gcactcctcg aagcactcgc gaatggccgc
      241 cacccagtat cgcaggccgc ccgcctccac gcccaaccgc tgcgacgccg accggtcgtc
      301 caggtcggtg acaagctcgg gcacgaagtc ggccgcgtcc accttgcccc ccggaaagac
      361 gtgcaggtcc gggaagtcga cgccgccggg acgctgaacc atgaacacct cggggccgcc
      421 gccaacgtcg cggacgagca ggatggtcgc ggcgagcctg atgttgtctt ttctaacggg
      481 ctgcatttgc aggaactgat atcaaattgc catctcttgt cgggctcggc atgccgcgct
      541 agtctgagac cgaccacgcg gccgagacct caaaatgaaa gaaaaatatt acaaggtcct
      601 cgtgcgtctt ccgccggaaa tgcgagagat gctcgcagag tcggcgcgac actaccggcg
      661 cagcaggaac tccgacattg tagcgcgtct gcaggagtcc ttcagaggca tagcagattc
      721 cgcccgggga cggggacttg cgccacccat gcacgaccag ttcgagcagc tgttcagggc
      781 cgacctcagc ccgcaggaac agagcctcgt cacggccttt cgccggctga gcagtgacca
      841 gcagacggcg ctgctcgccc tgctcaactg acgctgcgga ccgggacacc cctgatgtca
      901 ctttcaccct atcaccaccc caccacgatc tgcctggtgg acgacaatca atcctttctg
      961 gacagcatga tcctggagat gcccagcatg tgggcttgcc gggcgttcac ggacccccac
     1021 gcagctgcag agttcctgaa ccaacccatc gggctgccgc cgctggctga gcgctgcctt
     1081 tcccgggaga cctcaacggg ctccgacgcg gtcattcaca tcgacctcgg cctcatcgag
     1141 caggaggtca atcatccgga ccggttcagg cgcatatcgg tgctggtggc cgattacgcc
     1201 atgccatcca tgaacgggct ggagctttgc gccgcagcca gggatcccca tctgcgcaag
     1261 gccatgctca ccggcgtcgc ggatgaaaag ctggcagtcg aggccttcaa cgagggtctc
     1321 atccaccggt tcattcccaa acacgggacg tcggcgaccg ccatcgcccg ggaatacatc
     1381 gccgagctgc aggccgaata tttcgaacag tacacggccc tgcttcaggg cagcctgacc
     1441 gtgggcaagc ccggctttct gaccgatccc gacgtctcgc aatgggtcga ggcactcatg
     1501 cgcaccgagc ggctggtcga gtactacctg gtggatgacc cgccgggcct gctgatgctc
     1561 ggcagcgacg gcgcgatgtt cagagcgatg atcctggacc ccgttgaact gagccatcag
     1621 ctcgagatcg cgcgccggta tggcgcgccc gaccacatcg tgaagggcat cgcggagcgc
     1681 cggctggccg tgctgctcgc cggcgactcc ccggccgact attacggcgc cgagagcttt
     1741 ccgtgggaag agaacgtctg cgcggtcagc cccgtgggcg atgacgggaa gtggtttgtc
     1801 ggcgtggtgc gagacgcgcc ggtagacatc gatttcgacc cgaggctttc ctcctacgac
     1861 gcctacctgg caaccctgga gcgctgaagc ggcatcgcga ctggggccgg cgctacccga
     1921 ccggctggca cctcggtccg ggccgccggg acggccttgg accgtgtcag ccgccgtagc
     1981 gtgctttcag ccccccggcg taggcttcga gcttcccgag cagcctctcg cgtcgggcgt
     2041 cctctccggc cgagtcctgc cggatccgat ccagccgcgc gtcgaactcc ttcagcgtga
     2101 gccacggcgc atcctcagca ttgagcttgt cgcgcacgaa ctcgtgccag tcctgccgct
     2161 cctcctcgga gagcgactcc gcaaagctgc gggacttcag ccgggtcgag agcgaaacca
     2221 gccggcggtc cgaatagtcg aggtccaacc acttcccctg gccaagcgcc tcctgaaggc
     2281 tggcgcagcg accgcgatcc gcatcctgga gaaaggcgtc gtaaagcgcc gcttccacat
     2341 cgggtgctgc cggtgggggg cgatcgccat atacgcgcat gatcttctga gccagtccgg
     2401 gccgggcgag acgccgctgg cgttccttga tctctcgacg gtcgaacccg atgcgctccc
     2461 agttctcgcc ggtcatgacc tcgatgcccg ccacgaacgg gcagcgattg atgcggatct
     2521 ccttcagcgg ggggcgactg gtggaaccct ccttgaacag ctccgcacgt atctgctcgt
     2581 cggtccagcc gatcagcggc tccacgtctt cactgaggtc ggcgacgatg atggagttgc
     2641 tgttcttggg gtggcggcac agcgacacca ccggcgcggc gcagtagcgg ctgcgcggat
     2701 acatgcccga gacatgcagg catacgcgcg ctccgaacgg ttccaacagc tgcttgacct
     2761 gcttcttcga ccggccgttg aaatagtagt cgaacagctt cggctggcag tcgcgaatca
     2821 accgcgcaag cgcaaccgta gcgcgcacgt ccgacatggc atcgtgggcc tggccgtgct
     2881 cgaggtcatt ggccttggtg agcagctcga gccggtagac gggaagacct tcgtcgtcgg
     2941 tgggccagcg gatcccgtcg cgccgcagcg cgcccgtcgc ccggacgaga tcgatgatgt
     3001 cccagcgcga gttgccgttc tgccactccc gggcgtacgg gtcgatgagc atccggtaga
     3061 agccgaagcg aatgaactcg tcatcgaagc gaaggctgtt gtagccggcc acgcaggtct
     3121 ggggcgccga gaaaagctcg ttgatgcgcg tcatcgcctg ccactcgctg acgccttccc
     3181 ggcgggtgac atcaggggta atgcccgtca ccagcgtcgc ctcgggactc ggcagcacgt
     3241 cgtcggggag ctccacgtag gtgcagtact cgtccccgac cgggttgagc gacagatcgg
     3301 tgcgcagccc tgcgaactgg atcacgcgat cccagcgcgg ctctataccg gaggtttcaa
     3361 ggtcgtacca atagtagctg gctgccatcg cttcctataa ccttgccttc ccatgtcggc
     3421 ttccaacgca aagcgcatcg cgctccccgc tggccccatt ctcgcattgt tcgccggata
     3481 cctcatgtac tctaccggga cgaccccggc ggcggccata accttgggca taacgttgat
     3541 ctgcgtggtg tggtgggtgt tcgagcctat ccccatcccg gcaacgtcgg tgcttcccct
     3601 ggccatgctg cccctgctgg gggtgctgac gccgcagcag gtcgcccagg cttacggtca
     3661 tccgctcatc ctgctcatgc tcggcgggtt catcctctcc acagccctgg cccgcaacgg
     3721 ggcccaccgg cgcatcgcgc tgaccatggt ccgcgctttt ggcggcagca gcagccggcg
     3781 actggttttc gggttcatgg ccgccagcgc ggtgctgagc atgtggatct ccaacaccgc
     3841 gaccacgctg atgcttctgc ccgtggcgct cgcggtgctg gaaaaggccg aggatccgaa
     3901 gcttgccacg ccgctcctgc tgggcatcgc ttacgccgcc agcatcggcg gcatcggcac
     3961 gcccatcgga accccgccca acgtcgtgtt catgggcgtg tacaacgatc tcggcgtggg
     4021 tcagatcggg tttgcggact ggatgtcgtt tgcgatgccc gtggtgctgg tgctcctgcc
     4081 catcgccgcc gtgtggctga cccgtcgact gggacaccag ggcaccgtgg acgtccccga
     4141 ggtgggcgcg tggcaggccg gtgaggttcg ggtgctgatg gtcttcgccc tgactgccgt
     4201 cgcctggata acccgcagcg agccgttcgg aggctggacg gcactgctgg ggctggccca
     4261 gaccaacgac gccatggtcg ccctgctcgc cgtggtgatc atgttcgtcg tgcctgacgg
     4321 gaagggcgag cggttgctcg actgggagac cgctactcag atcccctggg gcatgctgat
     4381 tctgttcggc gcggggctga caatcgccgc ggcgtt
//
LOCUS       DBNHQR010000143         4009 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_774512, whole genome shotgun sequence.
ACCESSION   DBNHQR010000143 DBNHQR010000000
VERSION     DBNHQR010000143.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_774512"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="AC16VR_03190"
     CDS             <1..891
                     /locus_tag="AC16VR_03190"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6010190.1"
                     /translation="VPLALVLLLIYGTSFVTYRLSQRAVSPIVRLAAYLEDFDFNANL
                     EISRDLEPLRAHADAEVVSMIQAVEHFTTRLAAFVERERIFTRDAGHELRTPVAIFKG
                     SLDLLEQSGDRPAHEHKALARMRRTVSDMESLLETLLLLAREEQVEMPREDVSVNALV
                     AGQLELLQPMAERAENSLALHERAELRVRAPEKVLEIVLGNLIRNAINYTHGGRVDVT
                     VTDRSVQVGDTGVGMSGEELQNAFEPFYRVDESRGAIRGHGLGLAIVKRLAHQAGWSI
                     SAHSKPGEGTRVEVAFFPDD"
     gene            complement(893..1843)
                     /gene="folE2"
                     /locus_tag="AC16VR_03195"
     CDS             complement(893..1843)
                     /gene="folE2"
                     /locus_tag="AC16VR_03195"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007960861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HIR6010191.1"
                     /translation="MSTPKPVELASMPDIAGRPHAKPQGTLDWVGMSDIHQPILIRAG
                     NVNQRVQARVQVYVDLNDPHAKGIHMSRLYLLLDEHARTRPLSVAGLKMLLGSMHSSH
                     LDLSSRAFVQFDFEYELRQMALLSDNAGWSSYPITVKGTLADGKMTLELGVEILYSST
                     CPCSAALARQLIQQAFADRFDSGRPIDFADVNEWLGTEQGIVATPHSQRSVAKVITQL
                     ADDVTELPIAALIDTLETAIGTPVQAAVKRQDEQEFALRNGQNLMFCEDAARKLQAAL
                     NDDPSFNDFWLRVEHHESLHPHDAVSIVTKGIDGGYLPIP"
     gene            complement(1923..2621)
                     /locus_tag="AC16VR_03200"
     CDS             complement(1923..2621)
                     /locus_tag="AC16VR_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6010192.1"
                     /translation="MSRPEGSILLVEDNADLAETVGAFLEAKNYVVDYAADGLLAVQL
                     ATSEAFDAIVLDIMLPGIDGIEVCRRLRRDARLSTPIIMLTARDQLQDKLDGFDVGAD
                     DYLVKPFDMPELVARIEALIRRGRGVSAAYNVGELSMDLDTMSVFRGDTEILLSRTLF
                     DILKILLREHPKVVPRETIERELWGDDLPDSDTLRSHLYNLRKAVDRPFNEPLIETLA
                     GRGYRVRVPEPDAG"
     gene            2753..>4009
                     /locus_tag="AC16VR_03205"
     CDS             2753..>4009
                     /locus_tag="AC16VR_03205"
                     /inference="COORDINATES: protein motif:HMM:NF015089.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIR6010193.1"
                     /translation="MDATGFILQATYRIHAGVPVIYLFGRLESGETFLVRDHRQRPHF
                     YVRQEDARRAEALDGIALEASAARTFSGDAVCRVTVPTPSDAPPARERLHGLGVDTFE
                     ADVRFAMRYLIDRGIRGGCRIRGAASRGEGVGWVFDDPEVGAAEAGIEPVVLSFDIET
                     DAKADRLLGIAVYGAGVDEVLIVDPERRPMPERARGLPSERAVLEAFCQLVRDVDPDV
                     LTGWNVVDFDLTVLGRVAARVRHPFQLGRQAGALRIRPAQGYFGSGNATIPGRLVLDG
                     MDLLRGAFVRMEEYSLDAVAREVLGEGKTLDGDVRDRAGEIMQRYRQDLPGFARYART
                     DARLALDIVTRLDLMNLAFARSRLTGMTPDRVAASIASFDFLYLSALGKRGLVAPTVR
                     SDDSRVHAAQSGGQVFEPVTGVHRNVM"
BASE COUNT          627 a         1251 c         1400 g          731 t
ORIGIN      
        1 gtcccgctcg cgttggtcct gttgctgatt tacggcacgt ccttcgtcac ctaccgactc
       61 tcccagcggg cggtgtcacc catcgtgcgc ctggcggcct atctggagga cttcgacttc
      121 aatgccaatc tcgagatcag tcgcgacctc gaaccgctgc gcgcccatgc cgacgcggag
      181 gtcgtgagca tgattcaggc ggtggagcat ttcaccaccc ggctggcagc cttcgtcgaa
      241 cgtgagcgga tttttacccg cgacgccggc cacgagctgc gcacgccggt ggcgatattc
      301 aaaggcagcc tggatctgct cgagcagagc ggggatcgac cggctcacga gcacaaggcg
      361 cttgcccgaa tgagacgcac ggtctcggac atggaatcgc tgctggagac cctgctgctg
      421 ctggccagag aggagcaggt agagatgcct cgggaggacg tgagcgtcaa cgccctggtg
      481 gcgggccagc tcgagctgct gcagcccatg gccgagcgcg ccgagaacag cctggcgctg
      541 cacgagcgcg ctgagctgcg cgtgcgcgcc ccggagaaag tgctcgagat cgtgcttggc
      601 aacctgatcc gaaacgccat caactacacc cacggcggcc gcgtcgatgt gacggtaacc
      661 gaccgctccg tccaggtggg cgacacgggc gtcgggatgt cgggtgagga gttgcagaac
      721 gcgttcgagc cgttctaccg ggtggacgag agcagagggg cgatccgagg ccacggcctg
      781 ggcctagcca tcgtgaagcg gttggcccac caggccggct ggagcatctc ggcccacagc
      841 aagcctggtg aagggacccg tgtagaagtg gccttcttcc ccgacgacta ggtcagggga
      901 tgggcaggta gccgccgtcg atgcctttgg tgacgatgct caccgcgtcg tgggggtgaa
      961 ggctttcgtg gtgctcgacg cgcagccaga agtcattgaa ggacggatcg tcgttgagcg
     1021 cggcctgcag cttgcgcgcg gcatcttcgc agaacatcag attctgaccg ttgcgcaggg
     1081 cgaattcctg ctcgtcctgg cgtttcacgg cggcctgaac cggcgtgccg atggcggtct
     1141 ccagcgtgtc gatgagggcc gcgatgggga gctcggtcac gtcgtcggcc agctgggtga
     1201 tgacctttgc cacgctcctc tggctgtgag gcgtggcgac gatgccctgc tccgtgccga
     1261 gccactcgtt gacgtcggcg aagtcgatgg gccggccgct gtcgaagcgg tcggcaaacg
     1321 cctgctggat cagctgcctg gccagcgcgg ccgagcaagg gcaggtgctc gagtagagaa
     1381 tctccacccc cagctccagg gtcatcttgc cgtcggccag ggtgcccttc acggtgatgg
     1441 ggtaggagct ccagccggca ttgtccgaaa gcagcgccat ctggcgcagc tcgtactcga
     1501 agtcgaactg cacgaacgcc cggctgctca ggtcgagatg agagctgtgc atggagccga
     1561 gcagcatttt cagccccgcg acggacaggg gacgggtgcg ggcgtgctcg tcgagcagaa
     1621 gatacagccg ggacatgtgg atgcccttgg catgggggtc gttcaggtcc acgtacacct
     1681 ggaccctggc ctgaacgcgt tggttgacgt tgccggcccg gatcaggatg ggctgatgga
     1741 tgtcgctcat tcccacccag tccagggtgc cttgcggctt ggcatggggt cgtccggcga
     1801 tgtcgggcat ggaggccagc tcgacgggct ttggtgtgct catgggttat cccctcgatc
     1861 aatgatctga tgagatcaag ctagaggggc gccggtgaaa ggggtgtgaa aggcgttgcc
     1921 cgtcagccgg cgtctggctc aggaacccgc acgcggtatc cgcggcctgc cagagtctcg
     1981 atgagcggct cgttgaacgg ccggtcgacg gccttgcgca ggttgtagag gtggctgcgc
     2041 agggtgtcgc tgtcgggaag gtcgtctccc cacaattccc gctcgatggt ctcgcgtggg
     2101 accaccttcg ggtgctcgcg cagcaggatc ttgaggatgt cgaacagcgt acgcgatagg
     2161 aggatctcgg tgtcgccccg gaacacggac atggtgtcca gatccatgct cagctcgccc
     2221 acgttgtagg ctgcgctcac cccgcggccg cggcggatga gcgcctcgat gcgcgcgacc
     2281 agctccggca tgtcgaaggg tttgaccaga tagtcgtccg cgccgacgtc gaagccgtcg
     2341 agcttgtcct gaagctggtc ccgggcggtg agcatgatga tgggcgtgga gagcctggca
     2401 tccctgcgca ggcgccggca gacctctatg ccgtcgatgc cgggcagcat gatgtccagc
     2461 acgatggcgt cgaaggcttc gctggtcgcg agctgcacgg cgagcagccc gtccgcggcg
     2521 tagtccacca cgtagttctt tgcctccagg aacgcgccga ccgtctctgc taggtccgcg
     2581 ttgtcttcca cgaggaggat actgccttcg ggtcgactca tcggcgcatc ttagaggcag
     2641 ggtcacgccg gccgcaacgc cctcgaagcc ccttggtttc ggctcgcggc aatgggcaca
     2701 atgatctgtc cccccgacat gggcgctcgt atggcgtcga gccgatagat ccatggatgc
     2761 gaccggtttc atattgcagg ccacctaccg cattcacgcg ggggtgcccg tgatttacct
     2821 gttcggccgc ctggagtccg gcgaaacgtt tctggtgcgt gaccatcggc agcggcctca
     2881 tttctacgtg cggcaggagg atgcccggcg agccgaggcc ttagacggca tcgccctcga
     2941 ggcgtccgcc gccagaacgt tctccgggga cgcggtgtgt cgggtgaccg tgcccacccc
     3001 ctcagatgcg ccgcccgcgc gcgagcggct tcacggcctt ggggtcgaca ccttcgaggc
     3061 ggacgtccgc ttcgccatgc gctatctgat cgaccggggc atccgcggcg gctgccgcat
     3121 ccgcggggcg gcaagccgcg gagaaggggt aggctgggtg ttcgacgatc ctgaggtcgg
     3181 cgccgccgaa gccggtatcg agccggtggt tctttccttc gatatcgaga ccgacgccaa
     3241 ggcggaccgg ctgctcggca tcgccgtcta cggcgccggc gtggacgagg ttctcatcgt
     3301 cgaccccgag cgacggccca tgcccgagcg tgcccggggc ttgccctccg agcgggcggt
     3361 gctggaagct ttctgtcagc tcgttcgcga cgtcgacccg gacgtgctga cgggctggaa
     3421 cgtggtcgat ttcgacctga cggtgctcgg gagggttgcc gccagagtgc gccatccgtt
     3481 tcagctcggt cggcaggcgg gcgcgctccg catccgcccc gcccagggtt acttcgggag
     3541 cggcaacgcg accattcccg gccggctggt gctcgacggc atggacctgc ttcgcggggc
     3601 gttcgtgcgg atggaagaat attcgctgga cgccgtggcg cgggaggtcc tcggcgaggg
     3661 caaaacgctg gatggcgacg tgcgcgaccg tgcgggggag atcatgcagc gctaccgcca
     3721 ggatctgccg gggtttgcgc gctatgcgcg aacggatgcg cgcctggcgc tggacatcgt
     3781 cactcgcctc gatctcatga acctggcttt cgctcgcagc cggctgacgg gcatgacgcc
     3841 ggatcgggtg gctgccagca tcgcctcttt cgacttcctt tacctgtccg ctctgggcaa
     3901 gcggggcctg gttgcgccca cggtgcgttc cgatgacagc cgcgtccacg ctgcgcagtc
     3961 tggaggccag gttttcgagc ccgtgaccgg cgtgcatcga aacgtcatg
//
LOCUS       DBNHQR010000144         6861 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1597153, whole genome shotgun sequence.
ACCESSION   DBNHQR010000144 DBNHQR010000000
VERSION     DBNHQR010000144.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6861)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6861)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6861
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1597153"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            375..650
                     /locus_tag="AC16VR_03210"
     CDS             375..650
                     /locus_tag="AC16VR_03210"
                     /inference="COORDINATES: protein motif:HMM:NF016280.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HIR6010194.1"
                     /translation="MAEHSARNHSPGNEDVVGTFIERLTEQLASLVPPDSLRLVLDQA
                     RSALQRGLAEVDLVPRHELDAHLAALDRLTETTRDLERRVAALEADS"
     gene            661..2211
                     /locus_tag="AC16VR_03215"
     CDS             661..2211
                     /locus_tag="AC16VR_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004352315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HIR6010195.1"
                     /translation="MNDPDGHTLKSATEKSLTRTRAQAGMDTPAVTVETHLPGGLPAF
                     TLVGLPETAVRESRDRVKSAIDNCGLSFPNGKVVVNLAPADLAKEGARLDLAIAVSIL
                     AATGQLPHRVLEGFEFLGELGLSGELRPIRGCLCAALASATEVHERRLVLPSANAGEL
                     AMAPPGRVVPLAHLREVVRLLREPGYRPAAAPSAKPAANAERESRSLAEVIGQPGAKR
                     ALAVAAAGGHHLLMTGPPGTGKTMLARRLAGLLPALTEAEAREAALVYSAAGLPLEYP
                     FVPPFRDPHHTASGPALVGGGGHPQPGEISLAHRGVLFLDELPLFKPSVLEVLREPLE
                     TRSITIARARYRARFPASFQLVAAMNPCPAGRVCSESACRCTPEQVRRYRARISGPLL
                     DRIDLHVAVPPIPKALLLAGARDSSSGVDQTKAAIAGARRVQLSRQGRLNVDLTGADA
                     TRLTRLNTESRRLLANAVDRFQLSARGFHRVLKVARTVADIEGHVDVGPPHVAEALSY
                     RSVTWEGE"
     gene            complement(2216..2674)
                     /locus_tag="AC16VR_03220"
     CDS             complement(2216..2674)
                     /locus_tag="AC16VR_03220"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6010196.1"
                     /translation="MNFLLIGLALSGVALVAHAVPPGSEDEIRARTAPMGALCRAGEN
                     CSGFGAPAAMSAPAVAGAQAPAGAPAAAAQSGEQIYNQFCVACHAVGVANAPKLGDVA
                     AWADRIAKGMDVLMASTLNGINAMPAKGTCMTCTDADLQAAVEYMVDGSR"
     gene            complement(2787..3950)
                     /locus_tag="AC16VR_03225"
     CDS             complement(2787..3950)
                     /locus_tag="AC16VR_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009686373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HIR6010197.1"
                     /translation="MSAAEFDFGFDETLNMLRDSVRQFAADEIAPRAAQIDASNEFPR
                     DLWPKLGALGLLGVTVEEAYGGAGLGYLAHCIAMEEISRASASVGLSYGAHSNLCVNQ
                     IRRYATEEQKQRYLPPLISGDYLGALAMSEPGAGSDVVSMRLRAEDRGDSFVLNGSKM
                     WITNGPGADVLVVYATVDPSLGPRGITAFLIDKGTPGFSTAQKLDKLGMRGSDTCELV
                     FDDCEVSKTQVLGEVGQGVRILMSGLDYERVVLAAGPLGIMQSCLDTVLPYVHEREQF
                     GQPVGTFQLMQGKLADMYTTMNACRAYVYAVAQACDAGRTTRFDAAGCILFAAEKATW
                     MASEAVQALGGNGYINDYPTGRLLRDAKLYEIGAGTSEIRRMLIGRELFDATG"
     gene            complement(3973..5901)
                     /locus_tag="AC16VR_03230"
     CDS             complement(3973..5901)
                     /locus_tag="AC16VR_03230"
                     /EC_number="6.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016421 - CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HIR6010198.1"
                     /translation="MFDTLLIANRGEIACRVMRTATRMGLRTVAVYSDADAHAQHVRL
                     ADAAERIGPAPARESYLRIDAVLDAAERHGAGAVHPGYGFLSENAEFAAACEERGIVF
                     VGPSARAIRAMGSKIEAKRIVAAAGTPVVPGYQEADQSDAALSRAAAEIGYPLLIKAS
                     AGGGGKGMRLVREPDEFPAALAGARREAAAAFADELVLLERYLDAPKHLEVQILADQT
                     GHTLHLYERDCSVQRRHQKVIEEAPGPTVSEELRTLLGEAAVQAAKAIGYVGAGTVEF
                     ISQAGEFYFMEMNTRLQVEHPVTEAITGLDLVEWQLRVAAGEKLPFRQHEIPRNGHAV
                     EARVYAENPARRFLPSTGTLAHVAFPQEVRVDAGVATGDAVSVHYDPMLAKVIAHGPD
                     RDAAITSLERALARTEIAGVAHNVAYLRRLLAHPVFRGGDYTTGIAEEHADELLAEED
                     HLPAICAAIAAGRLGDSVGPWARRDGFRLNGPASFIQRFRRERKEVEVVLSEGQAQVD
                     GEVFDVADVSWNEGVLALRVDGNRVGARVVTAGPSLYVIIGGSTARFDPGDVEGAARS
                     LGSAAGNRVVSPMPGQVIAVSVKPGDRVKAGAVLAVVEAMKMEHSVAAPRAGRVASVN
                     CRVGDRVDEGVELVVLEE"
     gene            complement(5904..>6861)
                     /locus_tag="AC16VR_03235"
     CDS             complement(5904..>6861)
                     /locus_tag="AC16VR_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014852863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HIR6010199.1"
                     /translation="VKAATGEEVDAESLGGGDVHSRTSGVTDYLAADDVHALGIAREI
                     VAASQQRYRATVETRTPRPPAFAEEELYGVLPTDPRPPVDVREIIARLVDGSEFHEFK
                     ALYGETLVCGFAHIEGMPVAVIANNGILFSESALKGAHFIQLADRRGVPLLFLQNITG
                     FMVGKKYENQGIAKDGAKMVTAVACARVPKFTVVIGGSFGAGNYAMCGRAYGARMLWT
                     WPNARISVMGGEQAAHVLATVRRDGAAARGEEWPEAEQAAFMDDIRAQYETQGHPYFA
                     SARLWDDGVVDPADTRRVLGLALEVACREPLQPQSGFGVFRM"
BASE COUNT         1168 a         2416 c         2171 g         1106 t
ORIGIN      
        1 tgagtaaacc tctgtgctct tgctctaaaa accttgtgtc gcgaccgggt gatcgctccc
       61 cccttttctg cccaccgcag ggcggcagaa ctgacgggac gcgctcaaag gagcattttc
      121 cgtgccacca gatttttctt caaaaaaatc agatggttga aggaattctt ggctggaccg
      181 gcaggagata accggcttcc gagccgggct gttgcaccga aataaggcat ctgcaccaaa
      241 gtagcgcgcc agttcagagc cggcctgcac cggcccgggt cggtggctta cgcctgggcg
      301 aggattcccc agtcgaccgg cgacggcagc tgtgagtacc atcgtcgtca tccaaaccaa
      361 tgggcggacg cgcaatggcc gagcactcag ccagaaacca ctcacccggc aacgaggacg
      421 tggtgggaac gttcatcgag cgcctcaccg agcagctggc atccctcgtg ccgcccgaca
      481 gcctgcgcct ggtcctggac caggcccgct ccgcgctgca gcgcggcctt gccgaggtgg
      541 atctcgttcc ccggcacgag ctcgacgccc acctggccgc tctggacagg ctgaccgaaa
      601 ccacccgtga cttagagcgc cgggtcgctg ccctggaagc agattcctga tccgatcggg
      661 gtgaacgatc cggacggcca tacgctcaag tcggccaccg agaaaagcct gacccgtacc
      721 cgcgcccagg ccggcatgga cacgcctgcg gttacggtgg agacacatct tcccggcggc
      781 ctccccgcat tcaccctggt cgggttgccc gaaaccgcgg tcagagaatc ccgcgaccgg
      841 gtcaagagcg ccattgacaa ctgcgggctg tcgtttccca acggaaaggt ggtggtgaat
      901 ttagcgcccg ccgatctggc caaggaaggc gccaggctgg atctggccat cgcggtcagc
      961 atcctggcgg ccaccggcca gctgccccac cgggttctgg agggattcga gtttctgggc
     1021 gagctggggc tttccggcga gctgcgcccc atccggggct gcctgtgcgc cgcactcgca
     1081 tccgcaacgg aagtccacga gcggcgcctg gtgctgccgt cggccaatgc cggagagctt
     1141 gccatggcgc cccccggccg ggtggttcct ctcgcccacc tacgcgaggt ggtgcgtctg
     1201 ctgcgggagc caggctaccg gcccgcggcc gcaccatccg ccaagcccgc cgcgaacgct
     1261 gaacgggaga gccgctccct ggcagaggtg atcgggcagc ccggcgcaaa gcgagcgctg
     1321 gccgtcgccg ctgccggcgg gcatcacctg ctcatgacgg ggcctccggg aaccggcaag
     1381 acgatgctgg cgcgcaggct ggccggcctg ctgcccgcgc tgacggaggc cgaagcccgc
     1441 gaagccgcgc tggtctactc agcggcggga ctacccctgg aatatccgtt cgtaccccca
     1501 ttcagagacc cgcaccacac ggcttccggc cctgcgctgg tcggcggggg cggacaccct
     1561 cagccgggcg agatctctct ggcacatcga ggcgtgctgt tcttagacga gctgcccctg
     1621 ttcaaaccca gcgtgctgga ggtgctgcgc gagccgctcg agacccgctc gatcaccatc
     1681 gcccgagccc gctaccgggc ccgttttccg gcgagctttc agctcgtcgc cgccatgaat
     1741 ccctgcccgg cgggtcgggt ctgcagcgaa tccgcctgcc ggtgcacgcc ggagcaggtg
     1801 cgcagatatc gggcccgcat ctccggcccc ctgctggacc gcatcgatct ccacgtggca
     1861 gtgcccccga taccaaaggc gctgctgctg gcgggagccc gagactccag cagcggcgtt
     1921 gatcagacga aggccgccat cgcaggcgcc cgccgcgtcc agctgtcgcg ccaggggcgg
     1981 ctcaacgtgg atctcacggg ggccgacgcc accaggctga ccagattgaa cacggagagc
     2041 cgacggctgc tggccaatgc ggtggaccgc tttcagctat ccgccagggg attccaccgc
     2101 gtgctcaaag tggcgagaac cgtggcggac atcgaggggc atgtagacgt ggggccaccg
     2161 catgttgcgg aagcactgag ctaccggtcg gtgacctggg agggtgagtg atcggctacc
     2221 gggagccgtc taccatgtac tccacggccg cctgcagatc ggcgtccgtg caggtcatgc
     2281 aggtgccctt ggcgggcatg gcgttgatcc cgttgagcgt gctcgccatg agcacgtcca
     2341 tgcctttggc gatgcggtcc gcccacgccg ccacatctcc cagcttgggg gcgttggcca
     2401 cacccacggc atgacaggcc acacagaact ggttgtagat ctgctcgccg gactgagccg
     2461 cagcggccgg cgcccctgca ggggcttgag ccccagcgac cgccggggcc gacattgccg
     2521 ccggcgcccc aaagcccgag cagttctcgc cggctcggca aagtgccccc atcggcgcgg
     2581 tgcgggcacg gatctcgtcc tcgctgccgg gcgggaccgc atgagcgacc aaggcgacgc
     2641 cgctcagggc caggccaatc agtaagaaat tcataccctt cgatctgaca accaggcttc
     2701 gcaaatctct ttctccaggt gagatgggcg gaaaaaacat gccgagaggg cagcgcaagc
     2761 tgccgggcgc gaaattatag ccaaggtcat ccggtggcgt cgaacaactc ccgaccgatg
     2821 agcatgcggc gaatctcgct ggttcccgca ccgatctcgt agagtttcgc atccctgagc
     2881 agccgcccgg tgggatagtc gttgatgtag ccgttgccgc ccagggcctg caccgcttcg
     2941 ctggccatcc aggtggcctt ctcggcggcg aacaggatgc agccggccgc gtcgaaacgc
     3001 gtcgttcgcc cggcatcgca cgcttgcgca accgcgtaga cgtatgcccg acaggcgttc
     3061 atggtggtgt acatgtccgc gagctttccc tgcatgagct gaaaggtgcc taccggctgt
     3121 ccgaactgct ctcgctcgtg cacgtagggc agcacggtat cgaggcagct ttgcatgatg
     3181 cccagcggcc cggccgcaag aaccacccgt tcgtagtcca gcccgctcat cagaattcga
     3241 accccctggc ccacctctcc gagcacctgg gtcttggaca cctcgcaatc atcgaagacg
     3301 agctcacagg tatccgatcc ccgcataccc agcttgtcga gtttctgagc ggtgctgaaa
     3361 cccggcgtgc ccttgtcgat gagaaacgcc gtgatcccgc gaggacccag actcgggtcc
     3421 accgtggcgt aaaccaccag aacgtccgcc cccggaccgt tggtgatcca catcttgctg
     3481 ccattgagca cgaagctgtc gccccggtcc tcggctcgaa gcctcatgct gaccacgtcg
     3541 gagcccgcgc ccggctcgct catggccaga gcacccaggt aatccccgga aatcagcggc
     3601 ggcaggtagc gctgcttctg ctcctcggtg gcgtagcgac gaatctgatt gacgcaaagg
     3661 ttcgagtggg cgccgtagga cagcccgacg gaagccgacg cccgactgat ctcttccatg
     3721 gcgatgcagt gcgccaggta gccgagcccg gcgccgccgt aggcttcctc cacggtgaca
     3781 ccgagcaggc cgagggcgcc cagcttgggc cagagatccc tgggaaactc gttgctggca
     3841 tcgatctgcg ccgcgcgggg cgctatctca tcggccgcga actggcgcac gctgtcacgc
     3901 agcatgttca gggtctcgtc gaacccgaaa tcaaattctg cagcactcat gacacacggt
     3961 cctcccagga attcattcct cgagcacgac tagctcgacg ccttcgtcca cccgatcacc
     4021 aacgcgacag ttcacggatg ccacccggcc ggcacgcgga gcggcaacgg agtgctccat
     4081 cttcatggct tcgacgacgg cgagaacggc gcccgccttc acccggtcac cgggcttcac
     4141 cgagaccgcg atgacctgcc cgggcatggg cgatacgacc cggttacccg cagccgaacc
     4201 gagggagcga gccgcccctt cgacgtcgcc cggatcgaac cgggccgtcg acccgccaat
     4261 gatgacgtaa aggctgggcc cggccgtcac cacccgggct ccgacacggt tgccatccac
     4321 ccgcagcgcc agcacgccct cgttccagct gacatcggcc acatcgaaaa cttcgccgtc
     4381 gacttgggcc tgaccctcgg acagcacgac ctctacctct tttcgttcgc gtctgaaacg
     4441 ctggataaag gaggcaggcc cgttcaaccg gaacccgtcg cgtcgggccc agggcccgac
     4501 gctgtcgccg agccgcccgg ccgcgatggc cgcgcagatg gcgggcaggt gatcttcctc
     4561 ggcgaggagc tcgtccgcat gctcctcggc gatacccgtc gtgtagtcgc cgccccgaaa
     4621 gaccggatgg gccaggagcc gccgcaggta ggcgacgttg tgcgccacgc cggcaatctc
     4681 ggtgcgcgcc agggcccgct cgagggaggt gatcgcggcg tcacgatcgg gcccgtgggc
     4741 gatcaccttc gcaagcatgg ggtcgtagtg aacgctcaca gcgtccccgg tggccacccc
     4801 cgcatcgaca cgcacctctt gcggaaaagc cacatgggca agggttcccg tcgaaggaag
     4861 aaaacgccgg gccggattct ccgcgtaaac gcgcgcttcc accgcgtggc cgttacgcgg
     4921 tatctcatgc tgtcggaaag gcagcttttc gcccgcggca acccgcagct gccactcgac
     4981 gaggtccagc ccggtgatcg cttccgtgac cggatgctcg acctggagcc gcgtgttcat
     5041 ctccatgaag tagaactcgc ccgcctgcga gatgaactcc acggtgcctg ctccgacgta
     5101 gccgatggct ttggccgcct gcacggctgc ctccccgagc agggtacgca gctcctccga
     5161 aaccgtcggc ccgggcgcct cctcgatcac tttctggtgg cgcctctgca ccgagcagtc
     5221 gcgctcgtag aggtggagcg tgtggccggt ttgatccgcg aggatctgca cctcgaggtg
     5281 cttcggcgca tccaggtagc gctcgagcag cacgagctcg tctgcaaagg ccgccgccgc
     5341 ttcgcgccgg gcaccggcca gggcagccgg gaactcatcg ggttcccgca ccagacgcat
     5401 ccccttgccg ccgccccctg ccgaggcctt gatgagcagc gggtaaccga tctcggccgc
     5461 cgcgcgggac agcgccgcat cgctctgatc cgcttcctga tagcccggca cgacgggcgt
     5521 tcccgccgcg gccacgatgc gtttcgcttc gatcttcgag cccatggcgc gaatggcgcg
     5581 ggccgaaggg cccacgaaaa cgatgccgcg ctcctcgcag gcagcggcaa actccgcgtt
     5641 ctcggagaga aacccgtagc cggggtgaac ggcgcccgcc ccatggcgct ccgcggcgtc
     5701 gagcaccgcg tcgatgcgca ggtagctctc gcgggccggt gccgggccga tccgctcggc
     5761 cgcgtccgcc agacgcacgt gctgcgcgtg ggcgtcggca tcggagtaca cggcgaccgt
     5821 gcgtaagccc atgcgcgtgg cggtgcgcat caccctgcag gcgatctcgc cacgatttgc
     5881 tatcagcaga gtatcgaaca tggctacatc ctgaagacgc cgaagccgga ctgaggctgc
     5941 agcggttccc ggcaggcaac ctcgagcgcg aggcccagca cgcgccgggt atcagccggg
     6001 tcgacgacgc cgtcgtccca gagcctggcg ctcgcgaagt agggatgtcc ctgagtttcg
     6061 tactgagcac ggatgtcgtc catgaacgcc gcctgctcag cctccggcca ctcttccccg
     6121 cgagcggctg ccccgtcccg gcgtacggtg gccagcacgt gggccgcctg ctctcccccc
     6181 atgacggata tccgggcgtt cggccacgtc cagagcatgc gcgcgccgta ggcgcgcccg
     6241 cacatggcgt agttgccggc accgaaagag ccaccgatca cgacggtgaa tttcggtacc
     6301 cgcgcgcagg ccaccgccgt gaccatcttc gccccgtctt tcgcaatgcc ctgattctcg
     6361 tacttcttgc ccaccatgaa gccggtgatg ttctgcagaa agagcagagg caccccgcgc
     6421 cggtcggcga gctgaatgaa gtgcgcgccc ttgagcgccg actctgagaa caggatgccg
     6481 ttgttggcga tcacggcaac cggcatcccc tcgatgtggg caaacccgca gaccagcgtt
     6541 tctccataca gcgctttgaa ctcgtgaaat tccgatccgt ccaccagccg ggcgatgatc
     6601 tcgcgcacgt ccaccggggg gcgcggatcc gtgggcagca ccccgtagag ctcctcctcg
     6661 gcaaaggccg gaggtcgggg cgtgcgggtc tcgaccgtcg cgcgatagcg ctgctggctg
     6721 gccgcgacga tttcgcgcgc tatgcccagg gcatggacat cgtccgccgc cagatagtcg
     6781 gtcacgcccg acgtgcggct gtgcacgtca ccgccgccaa ggctttccgc atccacctcc
     6841 tcgccggtcg cggcctttac c
//
LOCUS       DBNHQR010000145         3548 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1281237, whole genome shotgun sequence.
ACCESSION   DBNHQR010000145 DBNHQR010000000
VERSION     DBNHQR010000145.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1281237"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="AC16VR_03240"
     CDS             <1..231
                     /locus_tag="AC16VR_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010200.1"
                     /translation="SRLVEKREHIALVVDEFGGMAGIVTMEDVIETLLGLEIVDESDQ
                     VYDMQAHARAQWARRAKAHGLTETDAVGPTSS"
     gene            complement(250..1299)
                     /locus_tag="AC16VR_03245"
     CDS             complement(250..1299)
                     /locus_tag="AC16VR_03245"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF040257.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent amine dehydrogenase family
                     protein"
                     /protein_id="HIR6010201.1"
                     /translation="MALKVFQWASGTVGRHAALAVLERDGLELVGLHTLSAAKAGRDV
                     GELLGVAATGLRATSELEAVLESDADVVVHAPLASLVYGEDPGRDVEDICRLLASGKN
                     VITVVGYMYPKVYGPELVARLEDACRAGKSTFHSTGLNPGWMGDLVPLTMSALSRTID
                     RVHVLEISNFQGYPSPEIMFESMGFGSSPEEFERSGERRRNWLNGLFRESVQMVADGL
                     GLELDDVVEEMTLEFAPRDLETAAGTVRQGTVAGQHWEWAGVLGGDKRVIHETVWRMH
                     ADVAPDWPQGRHAVTVHGVPRIHLEFEADYVSDGLLATAMHAVNAIPYVCQSEPGIRT
                     FLDLPWIMYRGAGAP"
     gene            complement(1323..2432)
                     /locus_tag="AC16VR_03250"
     CDS             complement(1323..2432)
                     /locus_tag="AC16VR_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008023997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HIR6010202.1"
                     /translation="MSTTTALAAVAWAPDDPFAFEEVELGPLGADEVLVRIVASGICH
                     TDVAVKEQSVKLPLPMVLGHEGSGIVEAVGSAVSHLMPGDHVVLSSDSCGVCRRCHAG
                     LPSYCDEFVERNLTGFRTDGTSPLSCRGEPLRGRFCGQSSFATAAISPGRSAVKVPQD
                     LPLELLGPLGCGLTTGVGTVMNALNPGPGSSIAVFGVGTVGLSAVMGAVLSGCEAIIA
                     VDPNLARLEVARELGATECIDAGREDVAADIVELTRGGADFSVECSGHPAAIDQAVAC
                     LGRPGWCAQVGAPTAGTRFPLDMDHVGFGRGIRGVVLGEANVHTFVPYLASLYRDGRL
                     PYDRLVRYYDFAEIDRAVHDSAVTGEVIKPILRMS"
     gene            complement(2429..>3548)
                     /locus_tag="AC16VR_03255"
     CDS             complement(2429..>3548)
                     /locus_tag="AC16VR_03255"
                     /inference="COORDINATES: protein motif:HMM:NF012472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6010203.1"
                     /translation="AGLSVMEMRVLGRTGVSVSKLCFGAGMFGYFGNPDERECARMVD
                     AAIDAGVNYFDTSDVYSHGESESILGKALVGKRDDVILATKFSLPMSDDPNHRGNSRR
                     WIAREVEASLKRLQTDYIDLYQVHRPDVTADIDETLGALSDLVRSGKVRMLGSSTFPA
                     EQIVEAQWVAEKRHRERFVVEQPPYSIFARRIEADILPTCQRYGMGVVAWSPLSQGWL
                     TGKWRRDAPPNDTNRQKLQPHLYDMARADNQRKLDLVEQLAAVASEAGISLMHMAIAF
                     VCAHPAVTSAIIGPRTPEQLQGLLDGADVTLGDDVLDAIDAIVAPGTTLSRDDDGYVA
                     PEIANKALRRRFVAEEVHEAARETLRNIEAYREQQGKK"
BASE COUNT          639 a         1198 c         1152 g          559 t
ORIGIN      
        1 tcgaggctcg tggagaagcg cgagcacatt gccctggtgg tggacgagtt cggcggcatg
       61 gccggcatcg tcaccatgga agacgtgatc gagacgctgc tcggcctgga gatcgtcgac
      121 gagtccgatc aggtctacga tatgcaggcc cacgcaaggg cgcagtgggc ccgacgcgca
      181 aaggcccatg gactcacgga aacggacgct gtcgggccga cgtcttcctg acgacgcctg
      241 gcggcaggct catggggcgc cagcaccacg gtacatgatc cagggcagat ccagaaacgt
      301 cctgattccc ggctcggact gacaaacgta gggaatggcg ttcaccgcat gcatggcggt
      361 ggcgagcaga ccgtcgctga cgtagtcggc ttcgaactcc agatgaatgc gcggcacgcc
      421 gtgcacggtg acggcatggc ggccctgagg ccagtcgggc gcgacgtcgg cgtgcatgcg
      481 ccagacagtt tcgtgaatca cccgcttatc gcccccgagc acgccggccc attcccaatg
      541 ctggccggcc acggttccct ggcggacagt cccggctgcg gtttcgaggt cgcggggagc
      601 aaactcgagg gtcatctctt ccaccacgtc gtcgagctcg agcccaagac cgtcggccac
      661 catctgcacg ctttcccgga agaggccgtt gagccagttg cggcgccgct ccccggaccg
      721 ctcgaactct tcgggtgacg atccgaaccc catggactcg aacatgatct ccggcgacgg
      781 gtaaccctga aagttggaga tctccaggac gtggacgcga tcgatcgtcc gcgacagcgc
      841 gctcatggtc aacggcacca ggtctcccat ccagccggga ttcaagcccg tggagtggaa
      901 ggtggactta cccgcgcggc aggcatcctc cagcctcgcg accagctccg gcccgtagac
      961 cttcggatac atgtagccca ccacggtaat cacgttcttg cccgaggcga gcagcctgca
     1021 gatgtcctcg acgtcacgac ccggatcctc gccgtaaacg agcgaggcca gcggcgcgtg
     1081 aacgaccacg tcggcgtcgg actccagcac ggcttcgagt tcgctggttg cccgcaggcc
     1141 ggtggcggca acgcccagca gctcacccac atcccggccc gccttggcag cggacagggt
     1201 gtgcaggcca acgagctcca ggccgtcacg ttcgagcacg gccagggcag cgtgacgccc
     1261 gacggtgccg ctggcccatt gaaaaacctt cagggccatg ggatcccctc caaatacctg
     1321 cgctaggaca ttctcagtat gggcttgatc acctcaccgg tgaccgcgct gtcgtgaacg
     1381 gcacggtcga tctcggcgaa atcgtagtat cgcaccagcc gatcataggg cagccgcccg
     1441 tcgcggtaga gcgaagcgag gtagggcacg aaagtgtgca cgttggcctc gccgaggacc
     1501 acgccgcgaa tgcccctgcc gaaccccaca tgatccatgt ccagagggaa acgggttccc
     1561 gcggttggcg cgcccacctg agcgcaccag cccggacgcc cgagacacgc caccgcctga
     1621 tcaatggccg cagggtgacc cgagcactcc acggagaaat cggcgccgcc ccgggtcagc
     1681 tctacgatat ctgcggcgac atcctcgcgc cccgcgtcga tgcactccgt ggcgccgagc
     1741 tcgcgggcca cctccagccg cgccaggttg ggatccacgg cgatgatcgc ctcgcagccg
     1801 gagagcaccg cgcccatgac ggcggaaagg cccaccgtgc cgacgccgaa tacggcgatg
     1861 ctcgaacccg gacccgggtt cagagcattc atcacggtgc cgaccccggt ggtcagaccg
     1921 cagccgagcg ggccgagaag ctcaagcggc agatcctggg gcaccttcac cgcgctgcgt
     1981 ccgggcgaga tagcggcggt cgcaaacgaa gattggccgc agaagcgtcc gcgcagaggc
     2041 tcgccccggc aggacagcgg actcgttcca tccgtgcgaa agccggtgag attgcgctcc
     2101 acgaactcgt cgcagtagga gggaagcccg gcgtggcagc gccgacagac gccacatgag
     2161 tcactgctga ggaccacgtg atcgcccggc atgaggtgcg agaccgcgct gccaacggcc
     2221 tcgacgatcc cggatccttc gtggccgaga accatgggca acggcagctt gaccgactgc
     2281 tccttgacgg caacgtcggt gtggcagatg cccgaggcta cgatccggac cagcacctcg
     2341 tccgcgccca gcggccctag ctccacctcc tcgaaggcaa acgggtcgtc cggcgcccag
     2401 gcgacggctg caagagcggt ggtcgtgctc atttctttcc ctgctgttcg cggtacgcct
     2461 cgatgttcct gagggtttcc cgcgcggcct cgtgaacctc ctcggccacg aaccggcggc
     2521 gcagggcctt gttggcgatc tccggggcca catagccgtc gtcgtcgcgg ctgagggtag
     2581 tccccggagc gacgatggca tctatggcat cgagcacgtc gtcgccgagg gtcacgtcgg
     2641 cgccatcgag cagcccctga agctgctcgg gcgtgcgcgg gccgatgatc gccgaggtca
     2701 ccgcgggatg ggcgcagacg aaggcgatgg ccatgtgcat caggctgatg cccgcttcgc
     2761 tcgcaaccgc cgccagttgc tccaccaggt cgagcttgcg ctgattgtcg gctcgggcca
     2821 tgtcgtacag gtgcggctgc agcttctgcc ggttggtgtc gttgggcggc gcgtcgcggc
     2881 gccacttgcc cgtgagccag ccttgcgaca acgggctcca cgcaacaacc cccatgccgt
     2941 aacgctgaca ggtgggcagg atgtccgctt cgatgcgccg ggcgaagatc gagtagggcg
     3001 gctgctcgac gacgaaacgc tcgcggtggc gtttctcggc aacccactgg gcttccacga
     3061 tctgctctgc ggggaacgtg gacgagccca gcatgcggac cttgcccgag cgaaccagat
     3121 ccgaaagggc gcccagagtc tcgtcgatgt cggcggtcac gtccggccga tgaacctgat
     3181 agagatcgat gtaatccgtc tgcagacgtt tcaggctcgc ctcgacctcg cgcgcgatcc
     3241 agcgccgcga gttgccgcga tggttcgggt cgtcgctcat cggcaggctg aacttggtgg
     3301 cgagaatcac gtcgtcgcgc ttgccgacga gcgccttgcc gagaatggat tccgactccc
     3361 catgggagta cacgtcggac gtgtcgaagt agttgacgcc tgcgtcgatg gcggcatcca
     3421 ccatgcgcgc gcattcgcgc tcgtcgggat tgccgaaata gccgaacatg cccgcaccga
     3481 agcagagctt gctgacactg acgcccgtac ggccgagaac ccgcatctcc atgacgctca
     3541 gccctgct
//
LOCUS       DBNHQR010000146         2258 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_964330, whole genome shotgun sequence.
ACCESSION   DBNHQR010000146 DBNHQR010000000
VERSION     DBNHQR010000146.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_964330"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..1096
                     /locus_tag="AC16VR_03260"
     CDS             59..1096
                     /locus_tag="AC16VR_03260"
                     /inference="COORDINATES: protein motif:HMM:NF024758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HIR6010204.1"
                     /translation="MVSGASILSSSRTSDYTLLALDDVPPNTAVYLGWNSTPVASSSG
                     LDLYRISHPSGAPQAYSKHSVSTSRPECRSWPRGPWIYSDDVVGATEGGSSGSPVVNE
                     AGQVVGQLSGACGYETSNVCSDRNATVDGAFAEYFNDVAQWLDPAPTSSGNNPPTASF
                     RWSGDPDVMFTDTSTDLDENDSISSWAWNFGDGLTSTATSPSHTYAYACNGTYNVTLT
                     VEDTNGASDSASNAVTITADDVCENLTIGVKDPLAGSIDESGRRPAVTFTINVATSDD
                     GGDPNGAVVSGDWGGLVKGSGSCTVNGSSCEVIKGNIRGSGDVTFTVTGIELSGYTFD
                     GTEVTGTVQVP"
     gene            1165..1614
                     /locus_tag="AC16VR_03265"
     CDS             1165..1614
                     /locus_tag="AC16VR_03265"
                     /inference="COORDINATES: protein motif:HMM:NF025559.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HIR6010205.1"
                     /translation="MSETRLTNLERTRIEMAYAVPLIRDLQRLLGAEVINDALSRRFD
                     DQIDATAAPGEKADFSRMEAGTATFAEGGALDYEIIGSDAEHFDLNITGCRYAEMMDE
                     LGARDIGHLLICNRDYPAAAKIGMALTRTQTRMQGAGYCDFRYRRRG"
     gene            complement(1615..1959)
                     /locus_tag="AC16VR_03270"
     CDS             complement(1615..1959)
                     /locus_tag="AC16VR_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HIR6010206.1"
                     /translation="MINIEHDYFSDEATPLAEIEAAGYHAVPLDFPAEENENHWHDFN
                     SLLYITEGELTVTYAETGETCTCGPGTKITGVPNVVHREKTEGYKALIGIPVPPAELT
                     QPINKPLPAPAG"
BASE COUNT          426 a          747 c          700 g          385 t
ORIGIN      
        1 ggcgaactcc ctgcaaacgt attttcactt cgaagattgc gacgggcctc aaccagccat
       61 ggtgtccggg gccagcattc tgagttccag ccgaacgagc gattacacgc tgctggcgct
      121 cgacgacgtg ccgccgaata cggccgttta cctgggctgg aacagcactc cagtcgccag
      181 ttcttccggc ttggatcttt accgtatcag ccacccgtca ggcgcgcctc aggcgtactc
      241 gaaacattca gtcagcacca gccgtcctga atgccgcagc tggccacgcg ggccgtggat
      301 ctacagcgac gacgtcgtcg gcgccaccga gggcggcagc agcggctctc cggtcgtaaa
      361 cgaggcaggc caagtcgtcg gccaattgtc cggcgcttgc ggctacgaaa cctccaacgt
      421 ctgcagcgac cgcaacgcta ccgtggacgg tgcgttcgcc gagtacttca acgacgtcgc
      481 tcaatggctc gatccggcac cgacgagcag tggcaacaac ccgcccacgg cgagcttcag
      541 atggtccggc gatccggacg tcatgttcac cgacacaagc accgacctcg atgaaaacga
      601 cagcatctcc agctgggcct ggaatttcgg cgacggccta accagcacgg caacgagccc
      661 gagccacacc tacgcctacg cctgtaacgg gacgtacaac gtcacgctca cggttgaaga
      721 taccaacggc gcaagcgact ccgcgtcgaa tgccgtcacg atcactgccg acgacgtctg
      781 cgaaaatctg acgataggcg taaaagaccc cctggcgggc agcattgatg aaagcggccg
      841 ccggccggcg gtgaccttta cgatcaatgt ggccaccagc gatgatggcg gcgacccgaa
      901 cggcgctgtg gtatccggtg attggggcgg actggtcaaa gggtccggct cctgcaccgt
      961 gaacggttcg tcctgcgagg tgatcaaggg caacatccgc ggcagcggtg acgtcacctt
     1021 taccgtcacg ggcatcgagc tctccggata caccttcgac ggcacagaag tcaccggcac
     1081 ggttcaggtc ccctgagcct cgggtagtca tggagcaggc gcggccgcat accgcgcctt
     1141 ccgcacagaa tgggggaacc acccatgagc gagaccagac tcaccaacct ggaacgtacg
     1201 cgcatcgaga tggcctacgc ggtgccgctg atccgcgatc tgcagcggct gctcggcgcg
     1261 gaagtgatca acgatgcgct ttcacgccgg ttcgacgatc agatcgatgc gacggccgca
     1321 ccgggcgaga aggccgactt ctcccggatg gaggccggta ccgcgacgtt cgccgagggc
     1381 ggcgcgctgg attatgagat catcggctcc gacgccgagc atttcgacct gaacatcacc
     1441 ggctgccgat acgcggagat gatggacgag ctcggtgccc gggacatcgg ccacctgctc
     1501 atctgcaacc gcgactaccc cgccgccgcg aagatcggca tggcccttac ccgcacccag
     1561 acgcgcatgc agggcgccgg ctactgcgac ttccgctacc ggcgccgggg ctgatcagcc
     1621 ggccggtgcg ggcagcggct tgttgatggg ctgggtaagc tctgccggcg gcacgggaat
     1681 gccgatcagc gctttgtagc cttccgtctt ctcccggtgc acgacgttcg ggacaccggt
     1741 gatcttggtt ccgggcccgc acgtgcaggt ctcgccggtc tcggcgtaag tgaccgtcag
     1801 ctcgccctcg gtgatgtaca gcaacgagtt gaaatcgtgc cagtggttct cgttctcctc
     1861 ggcggggaag tccaggggaa cggcgtggta gccggccgcc tcgatctccg ccaggggcgt
     1921 cgcctcgtcg gaaaagtagt cgtgctcgat gttgatcatg ttgctcccct catccggatc
     1981 gaacgtacac ccacattccg gcctctgtcc actcttgcat gatcgcaccg gtcgatcagg
     2041 tgtcacgcgc cctgtccggg ttgtcccacc gtttactccg ctctccggcc gatttaggct
     2101 ccgaggcagg gaggatacac gcatgaccaa cgatgaatta gcggtacctg aaacacccgc
     2161 agacgtcacg cccgcctggc tcaccagggc gctcggccag cgctggccgg ggatcgcggt
     2221 gcgcagcgtg gtcggcgaac cccgcggaga gggcatcg
//
LOCUS       DBNHQR010000147         3244 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1660795, whole genome shotgun sequence.
ACCESSION   DBNHQR010000147 DBNHQR010000000
VERSION     DBNHQR010000147.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1660795"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="AC16VR_03275"
     CDS             <1..454
                     /locus_tag="AC16VR_03275"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR6010207.1"
                     /translation="GYLQLEITETTVAQNRDTAIAILSSLRESGVQVAIDDFGTGYSS
                     LSYLQQFPIHTLKIDRSFVGDIRADHGDASIVNAIVAMARGLNLDLIAEGVENRTQLR
                     YLRSQGCSEVQGFIFSDPVPADQVKLMLQQNPFRAMVLDEQPEAATAV"
     gene            473..1696
                     /locus_tag="AC16VR_03280"
     CDS             473..1696
                     /locus_tag="AC16VR_03280"
                     /inference="COORDINATES: protein motif:HMM:NF004609.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase family protein"
                     /protein_id="HIR6010208.1"
                     /translation="MKGFTTKIVHSDRLEVIEHGSLHKPVHSTVAYGYDDARDLAAVF
                     QGTKAGYTYGRQVNPTVEALQTKISTMEQGLQTVCFSTGMAAIGTMLFALLRNGDHFV
                     SSSFLFGNTNSLFNSFSTHGVDVTFVDATDVDAVAAGINERTRLVFVETIANPRTQVS
                     ALEEIGKLCAERGLIYVVDNTMTSPYLFLPASVGASLVVNSLTKYIGGHGNALGGAVT
                     ELGNFDWADFSNIYDTYKSGAAEKWAITQIKKKGLRDFGAALAPEAAHHIAVGSETLG
                     LRLERQCANAAAMTAMLDAHPKVNAVYYPGLKQHPQHERASRLFRHPGALFSFELVPE
                     VDIWNFMNALDVIIKSSNLGDNRTLAIPVAHTIYYEMGPERRASMGIDDSLIRISVGI
                     EESEDLLGDLERALG"
     gene            1723..2490
                     /locus_tag="AC16VR_03285"
     CDS             1723..2490
                     /locus_tag="AC16VR_03285"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6010209.1"
                     /translation="MGRLKGKVAVITGGAGGIGAAAGRLFAEEGADVLLVDVDEKALE
                     ETVRGIGSNRVSYFVGDVTASADNAAMVACAEERYGGVDVLIANAGIEGDVAVPIVDY
                     DEARFDQVLGVNVKGVFLGLKSAIPAMARRGGGSIVITSSVAGVGGSPGMSAYVTSKH
                     AVIGLMRSAARECAGQNIRVNTVNPSPVETRMMRSIEEGIAPGGGEQVKSLMQSRIAL
                     QRYAEPEEIAKVMLFLASDDSAWVTGSVYMADGGMTA"
     gene            2527..>3244
                     /locus_tag="AC16VR_03290"
     CDS             2527..>3244
                     /locus_tag="AC16VR_03290"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HIR6010210.1"
                     /translation="MRQALLETNLGLPNRRSGKVRDLYDVTLDGGEEALLIIATDRIS
                     AFDVVMANGLPGKGIVLTQISRFWFDHFAGEVDHHLISTDVADVPGLSDAQREALAGR
                     IMLCRKTRVVPIECIARGYITGSGWKDYQRTGAVCGIELPEGLRNSDRLEEPLFTPST
                     KADTGHDENISFEEGVGIVGRELMDWLGDTTLRLYAKARDYALERGIILADTKFEFGQ
                     MPGREEPILIDEIFTPDSSRF"
BASE COUNT          623 a          993 c         1055 g          573 t
ORIGIN      
        1 gggctatctc cagttggaaa ttactgagac aaccgtcgcc cagaacaggg ataccgccat
       61 tgcgattctt tccagtctcc gggaatccgg tgtgcaggta gcgatcgatg attttggcac
      121 aggatactcg tcgctgagct acctgcagca gttccccatc cacacgctga agatcgaccg
      181 cagcttcgtg ggcgacatcc gcgcagacca tggcgatgcc tccatcgtca acgccatcgt
      241 cgccatggcg cggggtctca acctggacct cattgccgag ggcgtggaga accgcactca
      301 gcttcgctat ctgcgttccc agggctgcag cgaggtgcag ggcttcatat tcagtgaccc
      361 ggtgccggca gatcaggtca agctgatgct ccagcagaat ccgttccggg ccatggtcct
      421 ggacgagcag ccagaagccg ccacagccgt ctagcacggc gcgctgctac cgatgaaggg
      481 tttcaccacg aagatcgtgc acagcgaccg gctcgaggtc atcgagcacg gctcgctgca
      541 caagccggtg cactccacgg tggcatacgg gtacgacgac gcccgcgacc ttgcggcggt
      601 gttccagggc accaaagccg gctacaccta tggccggcag gtgaacccca ccgtggaagc
      661 cctccagacc aagatctcca ccatggaaca ggggctgcag accgtctgtt tctccacggg
      721 catggctgcc atcgggacca tgctcttcgc cctgcttcgc aacggcgatc acttcgtgtc
      781 gagctccttc ctgttcggca acaccaacag cctgttcaac tccttttcga cccacggggt
      841 ggacgtcacg tttgtcgatg ccaccgatgt ggacgccgtg gccgccggca tcaacgagcg
      901 tacccggctc gtgttcgtgg aaaccattgc caacccgcgc acgcaggtat ctgcgttgga
      961 ggagatcggt aagctgtgcg ccgagcgcgg cctcatttac gtggtggaca acaccatgac
     1021 gtcgccctac ctgtttctgc cggccagcgt cggcgccagc ctggtggtga acagcctgac
     1081 caagtacatc ggcggtcacg gcaacgcgct gggcggagcg gtgacggagc tcgggaattt
     1141 cgactgggcg gatttttcca acatctacga tacgtacaaa tccggggccg ccgagaagtg
     1201 ggccatcacc cagatcaaga agaaaggact gcgggacttc ggcgccgcgc ttgccccgga
     1261 agccgctcat cacatcgccg tgggctcgga gacccttggg ttgcggctgg agcgccagtg
     1321 tgccaacgcg gccgccatga cggcgatgct cgatgcgcac ccgaaggtca atgccgtcta
     1381 ctatcccggc cttaagcagc atccccagca cgagcgggcg agccgcctgt tccgccatcc
     1441 gggcgcgctg ttcagcttcg agctggttcc cgaggtggac atctggaact tcatgaacgc
     1501 gttggatgtg atcatcaagt ccagcaacct gggtgacaac cggaccctgg cgattcccgt
     1561 ggctcacacg atttactacg agatgggtcc ggagcggcgg gcgagcatgg gcatagacga
     1621 ttcgctgatc cgcatatccg tgggcatcga ggagagcgag gatctcctgg gggatctcga
     1681 gcgcgcgctg ggctgaatcg gtgtgcgggg gaagcgcaag gcatgggaag actgaagggt
     1741 aaagtggccg tcatcacggg gggcgccggt ggcatcggtg ctgctgcggg tcggcttttc
     1801 gccgaggaag gcgccgacgt cctgctggtc gatgtggacg agaaggcgct ggaggaaacc
     1861 gtgcgcggta tcggcagcaa ccgcgtcagc tactttgtcg gtgacgtcac cgcttctgcg
     1921 gacaacgccg ccatggtcgc gtgcgcggaa gagcgctacg gcggggtgga cgtgctcatt
     1981 gccaatgcgg ggatcgaagg cgacgttgcc gtgcccatcg tggactacga cgaggcgagg
     2041 ttcgatcagg tcctgggcgt caacgtcaag ggcgtcttcc tggggctgaa atcggccatc
     2101 cccgccatgg cgcggcgtgg cggcggcagc atcgtcatca cctcctcggt tgccggcgtc
     2161 ggaggatctc cgggcatgtc cgcttacgtc accagcaagc acgcggtgat cgggctgatg
     2221 cgatcggccg cccgggaatg cgccgggcag aacatccgcg tcaacactgt gaatccatct
     2281 cccgtggaga cgcgtatgat gcgaagcatc gaggagggca tcgcgccggg gggcggcgag
     2341 caggtgaaga gcctgatgca gtcgcgcatc gcgctgcagc gatacgccga gccggaggag
     2401 atcgccaagg tcatgctctt tcttgccagc gacgacagcg cttgggtcac cggctcggtc
     2461 tacatggcgg acggcgggat gacggcctga cattgaaagt ttgactcgat tggacatggg
     2521 ggaaaaatga gacaggcgct actggaaacg aatctcggtt tgccgaaccg acgctcaggc
     2581 aaggttcgcg atctctacga cgtcaccctc gacggcggcg aggaggctct gctgatcatc
     2641 gccaccgatc ggatcagcgc gttcgacgtc gtgatggcca acggcctgcc cggcaaaggc
     2701 atcgttctca cccagatctc acggttctgg ttcgaccact tcgccggaga ggtcgaccac
     2761 catctgattt ccaccgacgt ggcagacgtg cccgggctga gcgacgccca gagagaggcc
     2821 ctcgcaggac gcatcatgct gtgccgcaag acccgcgtgg tccccatcga atgcatcgcg
     2881 cgcggctaca tcaccggcag cggctggaag gattatcagc gcacgggtgc ggtgtgcgga
     2941 atcgagctgc ccgaggggtt gagaaacagc gatcggctgg aagagccgct gttcacgcca
     3001 tccaccaagg cggacaccgg tcacgacgag aacatcagct tcgaggaagg cgtcggcatc
     3061 gtcggccgcg agctcatgga ctggctcggc gacacgaccc tgaggttgta cgccaaggcg
     3121 cgcgactacg cgcttgagcg cggcatcatt ctcgccgaca cgaaattcga gttcggtcag
     3181 atgccgggtc gggaggaacc gattctgatc gacgagatct tcacgcccga cagctcgagg
     3241 ttct
//
LOCUS       DBNHQR010000148         7824 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_775208, whole genome shotgun sequence.
ACCESSION   DBNHQR010000148 DBNHQR010000000
VERSION     DBNHQR010000148.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7824)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7824)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7824
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_775208"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="AC16VR_03295"
     CDS             complement(<1..371)
                     /locus_tag="AC16VR_03295"
                     /inference="COORDINATES: protein motif:HMM:NF014722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO family protein"
                     /protein_id="HIR6010211.1"
                     /translation="MRSAALSVGLADLTFDNRFTAALPGDPESGNSRRRQIQGACYSR
                     VAPTPVAAPRLVAYSPETAELLGLGSADCLSAAFAEVFSGNRVLAGMDPHASCYGGHQ
                     FGNWAGQLGDGRAINLGEVIGT"
     gene            619..2739
                     /locus_tag="AC16VR_03300"
     CDS             619..2739
                     /locus_tag="AC16VR_03300"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HIR6010212.1"
                     /translation="MLRKTLHTIVWLGLLSGCSEGGPAETAGPVNTDTARIAAAQAEP
                     DQWLSHGGTYAEQRFSGLDEINDGNLDQLTLAWYFDFDTHRGQEGTPLMVDGVLYVTT
                     AWSKVFALDAATGRELWRFDPKVPGSAGYNACCDVVNRGAAFYQGRVYVGTIDGRLIA
                     LDASDGRVVWSTVTVPPDRPYTITGAPRAAKGKVFIGNGGAEYGVRGFVSAYDAETGE
                     LAWRFYTVPGDPELGPDGAASDEVLSSLAAPTWHGRAYLDYGGGGGTVWDAIVYDPEL
                     DQLYVGTGNGSPWVRALRSEGRGDNLFLSSVLALDPDTGAYLWHYQETPGETWDYTST
                     QQITLATMEIGGEPRRVLLHAPKNGFFYVIDRTTGKVVSADKFVPVNWAQRVDLQTGR
                     PVEAPGARFVDAPFTAVVGGAGAHNWQPMSYSPGTGLVYSPAQEVPFVYTQEEGFAFK
                     PGEWNLGVVLAGARLPADEATRREIVAANQGRLIAWDPVNQREAWRVEHGAPWNGGTL
                     ATAGNLVFQGLTDRTFRAYRATDGKQLWSFDTQNVIITAPMSFAVNGEQHVAVMAGNG
                     GGLPLTLPTFSGPLQTPNGRLLVFKLGGTAELPPFEPEPLKPVIADVALTPDELDTGF
                     DLFGRYCAACHGLETLSAGVLPDLRSSPALASSDAWRSIVHDGALETRGMIGFQARMS
                     ADQVEIIRGYVANEARKLESDADG"
     gene            2818..4122
                     /locus_tag="AC16VR_03305"
     CDS             2818..4122
                     /locus_tag="AC16VR_03305"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014869595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0004534 - 5'-3' RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HIR6010213.1"
                     /translation="MNLNLYGHDGRWLMVDCGITFAEPGESGPHIQMADPAFIASRRQ
                     RLCALIVTHAHEDHVGAVAHLWRQLQCPVYATPFTAEVLRRKLAEARLLDDVPIHVVR
                     SGDRLTLEPFDVEWVALTHSIPEPQALVIRTPAGSVFHTGDWKLDPSPVIGADYDEAR
                     CRQLADEPIGTMVCDSTNAMVEGRSISEGELYAGLLDLVSGARGRVIVGCFGSNVARL
                     HTLIRVAADTGRFACLLGRSLHNYYAAARASGVWESDLAFADAADIGYLPPDEVLVVA
                     TGSQGEPGAALDRLAADTHPLLNLEPRDTVLFSSRVIPGNEPAVEKLIRRLERFGVTV
                     IQDSELNMPIHASGHPARDELRDLYRWVRPDVALPVHGEEAHMQANAELAREVGVPRQ
                     MTGRNGDLFMLAPVRGVRRDAARVGRLGLDNGRLVHVGPPRR"
     gene            4130..4807
                     /locus_tag="AC16VR_03310"
     CDS             4130..4807
                     /locus_tag="AC16VR_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6010214.1"
                     /translation="MKIHADLSRRAVVKSPELPWVPSPLPGVDRRMLERDGGEIARAT
                     SIVRYAPESFFAQHEHSGGEEFFVLDGVFSDESGDYPAGWYVRNPIGSRHRPHTQSGT
                     TILVKLRQFQIGDDEYVRTDTNSGSWSPGEAEGVTIMALHAFGTERVSLARLATGTRM
                     PERAYPEGAEMFVLEGEIRDEHGVYPEGTWIRSPAGFSHRLRSPSGARLLVKTGHLPA
                     APASDVR"
     gene            5007..5396
                     /locus_tag="AC16VR_03315"
     CDS             5007..5396
                     /locus_tag="AC16VR_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6010215.1"
                     /translation="MTRFRTALALAVTLSAAAGCSERPADGPAAVRADDSPASLQSPP
                     AGQAAYAMACASCHDTGVAGAPRIGDDQSWSNRSPLWTAVLAEHAEDGYLGMPAMGGA
                     EELSERAVQDAVEYMLSATFPEAPPAD"
     gene            complement(5419..6927)
                     /locus_tag="AC16VR_03320"
     CDS             complement(5419..6927)
                     /locus_tag="AC16VR_03320"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF005565.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR6010216.1"
                     /translation="MLSTPTEANIAAICESLGIEADAAEVAVYRDLVEGSIAGCALVD
                     EMPDDMADWTGGERTWERPAAADNPFNAWYVKTSVAGAPDGPLVGRTLAIKDNVLVGG
                     VPLMNGTSILEGYVPPVDATIVTRILEAGGTIAGKSVCEAYCFSGGSHTASSGFVTNP
                     HDASRAAGGSSSGSGALVAGGVVDMAIGCDQGGSIRMPASFCGVVGMKPTHGLVPYTG
                     ILGMGPAIDHVGPMTATVRDNALLLEVIAGPDGLDSRQYGAREQAYSAGLEDGARGLR
                     VGVLSEGFGREDSEADVDAKVRAAAERFGRLGADVSEVSVPLHAVASAICFSVIQSTV
                     ETIFGHDGALISRLDPAVASLVAAQHAWRDRADELPANVKVMLMSAEYLRREHGWEYV
                     AKGVNQIRRLRAAYDAALAEVDLLLLPTTPMKATELPAPDADPATVLGLAFAPLSNTM
                     AFNNTHHPAISVPCGMSDGLPVGMMLVGGHFEESLLYRAAFAFEQHEDWREL"
     gene            complement(6972..7736)
                     /locus_tag="AC16VR_03325"
     CDS             complement(6972..7736)
                     /locus_tag="AC16VR_03325"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6010217.1"
                     /translation="MDYLTVDEAKRATGLRLVLTQGVPGPWGESAKALFDLKGVAYHP
                     VAQKGGAANEELLAWTGHRNAPIAILDDEPPRASWLEILFLAERLGESPRLLPEDIDQ
                     RMLVIGLSSEICSPGGFAWQSRMFLLQGMAKAAGDQPENFPMLKDYSYSQEAADRAVA
                     SVKPILDRLGRQLDMQAAAGSDYLVGGELTAVDVYWAFFSQLLVPLPEAICPMPGFLR
                     KSYGMLGTQLEEPAPASLLAHRDRILERHIALPLTF"
BASE COUNT         1316 a         2720 c         2572 g         1216 t
ORIGIN      
        1 gtgccgatca cctcgccgag gttgattgcc cggccgtcgc cgagctgacc cgcccagttg
       61 ccgaactgat ggcccccgta gcaggaggcg tgaggatcca tgccggcaag cacccggttg
      121 ccggagaaca cttccgcaaa tgccgcgctc aggcagtcgg cgctgccgag gccgagcagc
      181 tcggccgtct ccggcgagta ggcgaccagt ctcggcgcgg caacgggcgt cggcgccacc
      241 ctggagtagc aggcgccctg gatctgccgg cggcgggagt tgccggattc cggatcaccg
      301 ggcagagcgg ccgtgaaccg gttgtcgaac gtcagatcag ccagaccaac ggacagcgca
      361 gccgacctca cgggacccac gcgcgccgct cggaacgcag cacctgtcct ttcgctacca
      421 atgcagacat cgaccaaccc caggcaaacc cgccaacgac tggggcaatc ctaccctaaa
      481 gccacagata ggcgccgccc cggaccccac catgaccttg catgccggcc cagggctaca
      541 taaggttgcg gcctggtcgg tgaccaccag gattttctcg ctcctggcgc ccgccgaact
      601 atcatcacag gtgacaagat gcttcgaaag acactgcaca cgatcgtctg gctcggcctg
      661 ctgtccggct gctcagaggg cggacccgcc gaaacggcgg gacccgtcaa caccgacacc
      721 gcccgcatcg cggcggccca ggccgaaccc gaccagtggc tgtcccacgg cggcacctac
      781 gccgagcagc gattctcagg attggacgag atcaacgatg gcaacctcga ccagctgacg
      841 ctcgcctggt acttcgattt cgatacccat cggggccagg agggcacgcc cctgatggtg
      901 gacggcgtgc tgtacgtgac gacggcgtgg agcaaggtgt tcgctctgga cgccgctacg
      961 ggtcgggagc tctggcgctt cgacccgaag gtgcccggaa gcgccggtta caacgcctgc
     1021 tgtgacgtcg tcaaccgggg cgcagccttc taccagggca gggtatacgt cgggacgatc
     1081 gacggtcgtc tcattgccct ggacgccagc gacgggcgag tggtctggtc gacggtgacg
     1141 gtgccgcccg acaggccgta caccatcacc ggcgctcccc gagcggccaa aggcaaggtc
     1201 ttcataggca acggcggcgc ggagtacggc gtgcgcggct ttgtcagcgc ctacgatgcg
     1261 gagacgggcg agctcgcctg gcggttctac acggtgcccg gcgacccgga gctcggcccg
     1321 gacggcgccg cgtcagacga ggtgttgagc tccctcgccg cacccacctg gcacgggcgc
     1381 gcttacctcg attacggcgg cgggggagga acggtctggg atgccatcgt ctacgatccc
     1441 gagctcgacc agctctacgt gggcacgggc aacggctctc cgtgggtgcg tgcccttcgc
     1501 tccgaaggtc gcggcgacaa cctcttcctc tcctcggtgc tggcgctgga ccccgacacg
     1561 ggcgcctacc tctggcacta ccaggagacg cccggcgaaa cctgggacta cacgtcgacg
     1621 cagcagatca ccctggcgac catggagatc ggcggcgagc cccggcgcgt gttgctccac
     1681 gcgccgaaga acggcttttt ctacgtgatc gatcgcacaa ccggcaaggt cgtctccgcg
     1741 gacaaattcg ttcccgtgaa ctgggcgcaa cgggtggacc ttcagaccgg ccggccggtg
     1801 gaagcgccgg gcgcccggtt cgtcgacgcg ccctttaccg cagtggtcgg cggggcgggc
     1861 gcccacaact ggcaacccat gtcttacagc cccggcaccg ggctcgtcta cagcccggcc
     1921 caggaggtgc ccttcgttta cacccaggaa gaaggcttcg cgttcaagcc cggcgagtgg
     1981 aacctgggcg tcgtgctcgc gggcgctcgc ctgcccgcgg acgaggcaac gcggcgggag
     2041 atcgtggcgg ccaaccaggg ccggctcatc gcctgggatc cggtcaacca gcgggaggcc
     2101 tggcgagtcg agcacggagc cccgtggaat ggtggcacgc tggccacggc cggcaacctg
     2161 gtctttcagg ggctgacgga tcgcacgttc cgcgcatacc gggctacgga cgggaagcag
     2221 ctctggtcgt tcgacaccca gaacgtgatc atcaccgcgc ccatgagctt tgccgtcaac
     2281 ggagagcagc acgtggccgt gatggccgga aacgggggag ggctgccgct gacgctcccc
     2341 acgttcagcg gcccgctcca gaccccgaac gggcggctgc tggtattcaa gctgggaggc
     2401 acggccgagc tgccgccctt cgagcccgag ccgctcaagc ccgtcatcgc cgacgtggcg
     2461 ctgacgccgg acgagctcga caccggcttc gacctcttcg ggcgttactg cgccgcctgt
     2521 cacggtctcg agaccctgag cgcgggcgtc ctgcccgacc ttcgctcgtc gcccgctctg
     2581 gcatcatccg acgcctggcg ctcgatcgtc cacgacggcg cgctggagac gagaggcatg
     2641 atcggcttcc aggcgcggat gagcgccgat caggtcgaga tcatccgagg ctacgtggcc
     2701 aacgaggccc gcaagctgga aagcgatgcc gacgggtaga cgccggctcg acaagccgtg
     2761 acgcccaccg agcgggatct ctggctcctt ccgctgggcg gatgcggcga gatcggcatg
     2821 aacctcaatc tgtacggcca cgatgggcgc tggctcatgg tggattgcgg catcaccttc
     2881 gccgagcccg gggagtccgg accccacatt cagatggcgg acccggcttt catcgcctcg
     2941 cgccggcaac gactctgcgc gttgatcgtc acccacgccc acgaggatca cgtcggcgcg
     3001 gttgcccacc tctggcgcca gcttcaatgt ccggtctacg caacgccgtt cactgccgag
     3061 gtgctgcgtc gcaagctcgc cgaggcccgg cttctggacg acgtgcccat tcacgtggtg
     3121 agaagcggcg accgtctgac cctggagccc ttcgacgtgg agtgggtggc tctgacacat
     3181 tcgatcccgg agccccaggc gctggtcatc agaaccccgg cgggtagcgt gttccacacc
     3241 ggcgactgga agctggatcc ctcgcccgtg atcggggcgg actacgacga ggcgcgctgc
     3301 cggcagctgg ccgacgagcc gatcggcacc atggtctgcg actccaccaa cgccatggtg
     3361 gaagggcgtt ccatcagcga gggagagctc tatgccgggc tgctggatct ggtcagcggt
     3421 gccagaggcc gggtgatcgt cggctgcttc ggcagcaacg tcgcccggct gcacacgctg
     3481 attcgcgtag ccgccgatac cggccgcttc gcctgcctgc tcggcagaag cctgcacaac
     3541 tactacgccg ccgcccgggc gtccggcgtg tgggaatccg atctggcctt cgccgacgcc
     3601 gccgacatcg gctacctgcc gccggacgag gtcctcgtcg tcgccaccgg cagccagggc
     3661 gagcccggcg ccgcgctgga ccggctggcc gctgacacgc acccgctgtt gaatctggaa
     3721 ccccgggaca ccgtcctctt cagctcccgg gtcattcccg gcaacgagcc ggccgtggaa
     3781 aagctcatcc gccggctcga acgttttggc gtcacggtga ttcaggacag cgagctcaac
     3841 atgccgatcc acgcatcggg gcatcctgcc cgggacgagc tgcgcgacct ctaccgctgg
     3901 gtgcggccag acgttgccct ccccgttcac ggggaggagg cgcacatgca ggcaaacgcg
     3961 gagctggcga gggaagtcgg cgtgccgcgc cagatgaccg ggcgtaacgg cgacctcttc
     4021 atgctcgcgc ccgttcgggg cgtgcggcgg gacgccgccc gggtgggccg gctcggcctg
     4081 gacaacgggc gcctggtcca cgtgggcccg ccccgccgtt gatggcgcca tgaagatcca
     4141 cgcggatctg agccgacggg cggtggtaaa aagcccggaa ctgccttggg tgccttctcc
     4201 gctgccaggg gtggatcgac gcatgctcga gcgggacggt ggtgagatcg cccgggcgac
     4261 gtccatcgtc cgctacgcgc cggaatcttt cttcgcccag cacgagcact caggcgggga
     4321 agagtttttc gttctcgacg gggtcttttc ggacgagtcg ggcgactacc cggccgggtg
     4381 gtacgtccgc aaccccattg ggtccagaca ccgtccccac acgcagagcg gcacgactat
     4441 cttggtgaag ctgcggcagt ttcagattgg cgacgacgag tacgttcgca cagacaccaa
     4501 ctccggcagc tggtcgccag gagaggccga gggcgtcacg atcatggcgc tgcacgcttt
     4561 cggcaccgag cgcgtgagcc ttgcgcggct ggctacaggt acccggatgc cagagcgcgc
     4621 ctaccccgag ggcgcggaga tgttcgtcct cgaaggcgag atccgcgacg agcacggcgt
     4681 ctatccggag ggcacgtgga tccgcagccc ggcaggcttc tctcaccggc tgaggtcgcc
     4741 ctcgggcgcc cggctactcg tgaagaccgg gcatctgccg gccgcgccgg cgtcagacgt
     4801 caggtaagcc gccttgccca cctgctccac caagacgtcc cgagcgcctt agagatcggc
     4861 gatgcctttg ccgtccttga agttgcaggc gtagaacttt ctgaccagtc tggtttctgc
     4921 cgcgccagga ggctcggtca aacgcccgct tgcgcgccgg ccgcatgata cttagccttt
     4981 actcaacgac cagtcgagaa accgccgtga ccagatttcg caccgccctt gctcttgccg
     5041 tcacgctgtc cgcggcggcc ggctgctccg aacgtccggc agacgggccc gccgccgtcc
     5101 gagccgacga ctctccggcg tcgctccaat cgccgccggc cggtcaggcc gcgtatgcga
     5161 tggcatgtgc gagctgccac gacacagggg tcgcgggcgc gccccgcatc ggggacgatc
     5221 agagctggtc aaaccgctca ccgctctgga cggccgtgct ggccgagcac gccgaggacg
     5281 gctatctggg aatgcccgcc atgggcggtg ccgaggaact cagcgagcgg gcagtgcagg
     5341 acgccgtcga gtacatgctc tcggccacct ttccagaggc acccccggca gattgacccc
     5401 tgcgcctgac cggggcgctc aaagctcgcg ccagtcctcg tgctgctcga aggcgaaagc
     5461 ggcgcggtag agcagggact cctcgaagtg gccgcccacc agcatcatgc ccacgggcag
     5521 cccgtcactc atgccgcagg gcacggaaat cgccgggtga tgggtattgt tgaacgccat
     5581 ggtgttgctc agcggggcga atgccagccc gagcacggtg gccgggtcgg cgtccggtgc
     5641 cggcagctcg gtggctttca tgggcgtggt cggcaacaac agcagatcca cttcggccaa
     5701 cgccgcatcg taggccgccc gcaaccggcg gatctgattg acgcctttgg ccacgtattc
     5761 ccagccgtgc tcgcgacgca gatattcggc cgacatcaac atcaccttga cgtttgcagg
     5821 cagctcgtcg gcccggtcgc gccaggcgtg ctgagcggcc accaggctgg caacggcagg
     5881 atccaggcgg cttatcagag cgccgtcatg accgaatatg gtttccacgg tggactgaat
     5941 caccgaaaag cagatggccg aagccaccgc gtggagcggc acggaaacct cactgacgtc
     6001 cgcccccagg cggccgaagc gctcggcggc cgcgcgcacc ttggcatcca cgtccgcttc
     6061 tgaatcctcg cggccgaacc cctcgctcag cacgcccacg cgcaggcccc gcgcaccgtc
     6121 ttccagccca gccgagtatg cctgctcccg cgctccgtat tgccgactgt ccaggccgtc
     6181 cgggccagcg atgacctcga gcagaagggc gttgtcacgc acggtagcgg tcatgggccc
     6241 gacatgatcg atggccgggc ccatgccgag gatgcccgtg tacggcacca gcccgtgggt
     6301 gggcttcatg cccaccacac cgcagaacga agcgggcatg cggatcgacc ccccctggtc
     6361 gcagccgatg gccatgtcca cgacgccgcc tgcaacgaga gcgccgctgc ccgacgagga
     6421 accgccagcg gctcggctcg catcgtgggg gttcgtgacg aatcccgagc tggcagtgtg
     6481 gctgccgccg gaaaagcagt aggcctcgca gaccgacttg ccggcaatgg tcccacccgc
     6541 ctcgagaatc cgggtcacga tggtggcatc gaccggcggg acgtagcctt cgaggatgct
     6601 ggtgccgttc atcagcggca cgccgcccac gagcacattg tccttgatgg ccagggttcg
     6661 cccgaccagc gggccgtcgg gcgcgccggc gacgctggtc ttcacgtacc aggcgttgaa
     6721 agggttgtcg gcagcggccg ggcgctccca cgtgcgttca cccccggtcc aatccgccat
     6781 gtcgtccggc atctcgtcca ccagggcaca ccccgcgatg gaaccctcca ccaggtcacg
     6841 gtaaacggcg acctccgcgg catcggcctc gatgccaaga gattcacaga tggctgcgat
     6901 gtttgcttcg gttggcgtgc tgagcatggt cttctccctc ccagagcggc gaagccgcgt
     6961 gaaaccaatc gtcaaaaagt cagcggcagc gcgatgtgcc gctcgagaat ccggtcgcga
     7021 tgggccagca ggctggccgg cgccggctcc tcgagctggg ttcccagcat cccgtaagat
     7081 ttccggagaa agcccggcat gggacagatg gcttccggca gcggcacgag cagctgtgag
     7141 aagaacgccc agtaaacgtc caccgccgtc agctcgcctc ctaccaggta gtcgctgccg
     7201 gcagcggcct gcatatccag ctgccgccca agccggtcga gtatgggctt cacgctcgcg
     7261 actgcccggt cggccgcttc ctggctgtag ctgtaatcct tgagcatggg aaagttttcc
     7321 ggctgatcgc cggccgcctt ggccatcccc tggagcagaa acatgcggga ctgccaggca
     7381 aagccgccgg gagagcagat ctcgctggaa agcccgatga cgagcatgcg ctggtcgatg
     7441 tcctcgggca gaagccgggg cgactcgccg agccgctcgg cgagaaagag gatctcgagc
     7501 cagctcgccc gcggcggctc gtcgtcgaga atggcgatgg gcgcgtttcg atggcccgtc
     7561 caggccagca actcctcgtt tgccgccccg cccttctgcg ccacgggatg gtaggcaacg
     7621 cccttcaggt cgaacagcgc cttggccgac tcaccccagg ggccaggcac gccctgggtg
     7681 agcacgagcc gcaaacccgt tgcccgtttt gcctcgtcaa ccgtcagata atccatctcc
     7741 cctctacccc agcgatgtcg atcccaagtt ataccccgaa aacgtcaggc atctcgaccg
     7801 cggcgcgacc cggtctgcgc gacc
//
LOCUS       DBNHQR010000149         4545 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_710676, whole genome shotgun sequence.
ACCESSION   DBNHQR010000149 DBNHQR010000000
VERSION     DBNHQR010000149.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4545)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4545)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4545
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_710676"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="AC16VR_03330"
     CDS             <1..401
                     /locus_tag="AC16VR_03330"
                     /inference="COORDINATES: protein motif:HMM:NF015669.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HIR6010218.1"
                     /translation="WYVPGGLAGDQPEAEPAAASLVGTRWLWVGSTIGSADVEVAEPH
                     RYTLLLNADGGAEVRLDCNQGRGSYQLEGGALRLGPIMSTRMACPEDSQDFEFGQQLD
                     GVAAFSMDGGALELRLNSEGDVMRFRAETD"
     gene            complement(458..1051)
                     /locus_tag="AC16VR_03335"
     CDS             complement(458..1051)
                     /locus_tag="AC16VR_03335"
                     /inference="COORDINATES: protein motif:HMM:NF024978.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HIR6010219.1"
                     /translation="MISCSSPGIESFHDYNPNVNFARYQTWSFISTNPMIVSTAAGAV
                     NPLLEGRIMRAVRTDLAGKGFRYADNQENADFVVSFTVGSREQIKVDQYPASYQVGYS
                     RYHRYGYGGYGYGMSYGTETRVRQYTEGQLAVDVFDVATRSPAFHGSATKRISDSDRE
                     NPEALIAAVVGEALTGFPPGSAAGVAQPQLIPYEDQN"
     gene            1252..2079
                     /locus_tag="AC16VR_03340"
     CDS             1252..2079
                     /locus_tag="AC16VR_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010220.1"
                     /translation="MSLRKFWVVVSGLLLGSVALAGESPKFGDTVSLRLGGMIHKADA
                     TFSDTRIDRPPVELDLKGLGMDKRYDSFWAGLEWQFAKRWGLTLSYSDFSTDGERSVA
                     EGGNFGDLEWSVGAELTSEMDMKIYIVDVTWDFVKTERSHFGLGLGAHVADFSMEIDL
                     SVEGQIGDDTAIIAGGTETAGITAPLPNISMRGGHRFGDNFYLGGTAGYFSLKVGDID
                     GELITARGSLEWRPHKRFGLGIGYQFVSFDVQEKKETKQERYDADFYGPIFFVSAGF"
     gene            complement(2129..3103)
                     /locus_tag="AC16VR_03345"
     CDS             complement(2129..3103)
                     /locus_tag="AC16VR_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR6010221.1"
                     /translation="MSANCSDWLKAGLLCAALAVLGSCVTRAEQVASQSDMSWFARTV
                     SNSPLADADPDDFYEDASKMVRTDIEFHGVVLSEEGNPLKGARVRTSVFDRLADPFEF
                     PFFAFAAREPVFTGDDGRFSVRERGAGMYVTVEVPGWAPVTAPRKLYVYAEGLNQTLP
                     FPTREAPEEFRFERRPPEEELRPIQTGALRLPEDGVPLEVSLRQVAPYGVEPGTGEAI
                     ITCERTADDAAPDDLFDWWCELTVPGGGFQPFRIDMDQAPEEGYVESSRLEYRAADPR
                     WDDRAERSLIIRFADGVYGFIEVSMRMDGDFYVAFSGHWNPTGSTWLD"
     gene            complement(3084..>4545)
                     /locus_tag="AC16VR_03350"
     CDS             complement(3084..>4545)
                     /locus_tag="AC16VR_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010222.1"
                     /translation="DANTPAPDSSRDLEDFTRLHVEIDDLGSLPGDAVLTLAWASGST
                     AELNLFRNEDPDCSRRYLLDAEAGRRQLSAPHGTPIGTVGANALKIPLQTLGEPGLDG
                     AGYCFLLEVASPGQGHLVATIADGEGVLARSEPIAAELRHIKSLYQRTTMTWPEEILA
                     PWAYTTEAPPVPDLQWSKDPQGHEFEPGWWETEDHVVWVYGWLKSGEGLREMATTQAA
                     ETIFKRLWHRGYRGRLTFFHWPTVKPRYAYGLLESEYRAYKSAPALVDFVSQIPKGKR
                     IHVTAHSLGCVLVTEAIKLGLEAEEALLQVGAIPAAAYDTRPVLTLPDMADVVTPWLP
                     EEGGYHGYIDETPTPVYTMYNYADVTFFGWNIAQKQMKPTAKRGGYRYAWEPDAPQGE
                     RARLYYKTGWFSSKYRLVDDPHEIMAFIAKAKSHAIGAETRVQGHVRRVFDLARKPHD
                     FHTGHVVGWTRPVHETTAFYNLLLDVWNIAYVSELL"
BASE COUNT          758 a         1395 c         1548 g          844 t
ORIGIN      
        1 tctggtacgt ccccggcggc ttggcaggcg atcaaccgga agccgagccg gcggcggcaa
       61 gcctcgttgg gacccgttgg ctctgggtgg ggtcgacgat cggctcggcg gatgtcgagg
      121 tggcggagcc ccatcgctat accctgctgc tgaacgccga tggcggcgcg gaggtccgtc
      181 tcgactgcaa tcagggccgt ggcagctacc agttggaggg cggggcgctc cggctcggcc
      241 cgatcatgtc gacaaggatg gcctgccccg aggactccca ggacttcgag ttcggccagc
      301 agctcgacgg ggtcgctgcg ttctcgatgg atggcggcgc cctggagctg cgcctgaatt
      361 ccgagggcga cgtgatgcgc tttcgcgccg aaaccgactg atcgagcgcg cctgagctcc
      421 gatcaccgga gcccgcgcgt cagtcgagtt tgtcagatca gttctgatcc tcatacggta
      481 tcaactgagg ctgcgccacg ccggcggctg acccgggcgg gaagcccgtg agcgcctcgc
      541 ccacgacggc ggcgatcagc gcttccggat tttcccggtc gctgtcggat atccgcttgg
      601 tggccgagcc gtgaaaggcc gggctgcgtg tggctacgtc gaagacgtcc acggcgagtt
      661 gtccttccgt gtactgccgg acgcgggttt ccgtgccgta gctcatgccg tacccgtagc
      721 cgccgtaacc gtagcgatga tagcggctgt agccgacctg gtagctggcc ggatactggt
      781 ccaccttgat ctgctcccgc gatccgacgg tgaatgaaac gacgaagtcc gcgttctcct
      841 gattgtcggc atagcggaat cctttccctg ccaggtcggt ccgcaccgcg cgcatgatcc
      901 ggccttccag caacggattg acggcgcccg cggcggtgga gacgatcatg ggattggtgg
      961 aaatgaacga ccacgtctga taccgcgcaa agttgacgtt cgggttgtag tcgtggaatg
     1021 actcgatccc cgggctgctg caggaaatca ttgcaacaac ggcggctgcg aaagcggatc
     1081 gggcaaggtt ggcggcagac atgcgtgctc cctttgtgtg tgctttgtga cgcgggggcg
     1141 atcatagcgg caaacggagg tcggcacacg tccgcgatcc gcagctgcac ggctatgtat
     1201 aatgccggct gcagaagtgc tgggacgttg caaagcacgt aggagatgtc gatgagcttg
     1261 cggaaattct gggtggtcgt gtctggcctt ctgttgggtt ctgtcgccct ggcgggtgag
     1321 agccccaagt tcggagacac ggtctcgctg cggctgggcg gcatgatcca caaggccgat
     1381 gcgaccttct cggatacccg gatagaccgc ccgcccgtgg agctcgacct gaaggggctg
     1441 ggcatggaca aacgctacga ttcgttctgg gccggcctcg aatggcagtt tgccaagcgg
     1501 tggggcctga cgctgagcta ctccgatttc agcaccgacg gggaacgcag cgtggccgag
     1561 ggtggcaact tcggcgatct ggagtggtcg gtgggtgccg agttgacgtc cgagatggac
     1621 atgaagatct acatcgtcga cgtcacctgg gatttcgtga agaccgaacg cagccatttc
     1681 ggtctcggtc tgggtgcgca cgttgccgat ttcagcatgg aaatcgatct gagcgtcgag
     1741 ggtcagattg gcgacgatac agccatcatt gctggcggga ccgaaaccgc gggcataacg
     1801 gcgcccctgc ccaatatctc catgcgaggc ggccaccgct ttggtgacaa cttctacctg
     1861 gggggtaccg ccggatactt ctccctcaag gtcggggaca tcgacggcga gctgatcacg
     1921 gcacgggggt ctcttgaatg gcggccgcac aagcggttcg gtctcgggat cggctaccag
     1981 ttcgtcagct tcgacgtgca ggaaaagaaa gagaccaagc aagagagata cgacgccgac
     2041 ttctacggcc ccatcttctt cgtctcggcc gggttctaag agtcgcctct caggcagcta
     2101 cggacgccgc gcggcggcag cgaaacgtct aatccagcca ggtggagccg gtcgggttcc
     2161 agtgcccgct gaacgctacg tagaaatctc cgtccatgcg catggatact tcgatgaaac
     2221 cgtacacccc gtctgcgaaa cggatgatga gagagcgttc tgcgcggtcg tcccagcggg
     2281 gatcggcggc gcggtactcg agacgactgg attccacata accctcctcg ggcgcctgat
     2341 ccatgtcgat ccggaaaggc tgaaatccgc cgcccggcac ggtcagctcg caccaccagt
     2401 cgaagagatc gtcaggggcg gcgtcgtccg cggttcgctc gcaggtgatg atggcttcac
     2461 cggtgcccgg ctccacgccg tagggtgcta cctggcgcag cgaaacctcc aggggcacgc
     2521 cgtcttcggg tagccgcaac gcgccggtct ggatgggcct gagctcttcc tccggaggcc
     2581 gccgctcgaa ccggaactcc tccggcgcct cccgggtggg aaacggcaac gtctggttca
     2641 atccttccgc gtagacgtag agcttgcggg gcgcggtcac gggcgcccag ccggggacct
     2701 cgacggtcac gtacattccc gccccccgtt cgcgaacgct gaaacggccg tcgtcgcccg
     2761 tgaagactgg ctctcgggcg gcgaacgcga agaagggaaa ctcgaacggg tcggccaggc
     2821 ggtcgaagac ggaagtccgc acgcgcgctc ccttgagcgg gttgccctcc tcgctgagca
     2881 ccacgccatg aaactcgata tccgttcgga ccatcttgga cgcgtcctcg tagaaatcgt
     2941 ccgggtctgc gtcggcaagg gggctgttgc tcaccgttct tgcgaaccag gacatgtcgc
     3001 tctgggacgc cacctgctcg gcccgggtca cgcagctgcc cagcaccgcg agggctgcgc
     3061 agaggagtcc ggctttcagc caatcagagc agttcgctga cataggcgat gttccagacg
     3121 tcgaggagga ggttgtagaa cgccgtggtc tcgtggaccg gtcgcgtcca gcccaccacg
     3181 tggcctgtat ggaagtcgtg cggctttcgg gccaggtcga acacccgtcg gacgtgcccc
     3241 tgaacgcggg tttccgcacc gatcgcgtgg gatttggcct tggcgatgaa ggccatgatc
     3301 tcgtgggggt cgtcgaccaa ccgatatttg gagctgaacc accccgtctt gtagtagagc
     3361 cttgcgcgct cgccctgcgg tgcgtcgggt tcccaggcgt agcgataccc gccccgcttg
     3421 gccgtcggct tcatctgctt ctgcgcgatg ttccagccga agaaggtgac gtccgcgtag
     3481 ttgtacatcg tgtacaccgg ggtcggggtc tcgtcgatgt aaccgtggta gccgccctcc
     3541 tccggcagcc acggcgtcac cacgtccgcc atgtcgggca gggtgagaac cgggcgggtg
     3601 tcgtaggcgg cggccggtat ggcgccgacc tgcaggagcg cctcctcggc ttccagaccc
     3661 agcttgattg cttcggtgac gagcacgcag ccgaggctgt gggcggtgac gtggatgcgt
     3721 ttgcctttcg ggatctggct gacaaagtcg accagggcgg gggcggactt gtaggcgcgg
     3781 tattcgctct cgaggagccc gtaggcgtag cgcggcttca ccgtgggcca gtgaaagaac
     3841 gtaagccgcc cccggtagcc gcggtgccag agccgcttga aaatcgtctc ggcggcctgc
     3901 gtggtggcca tctcccgcaa tccctcgccg cttttgagcc agccgtacac ccagaccacg
     3961 tgatcttccg tctcccacca gccgggctcg aactcgtggc cctgggggtc cttgctccat
     4021 tggagatcgg gtaccggagg cgcctcagtg gtgtacgccc atggcgccag gatctcttcc
     4081 ggccaggtca tggtggtccg ctggtacagg gacttgatgt ggcgaagctc cgcggcgatg
     4141 ggctcggaac gggccaggac gccttctcca tcggcgatgg tggcgaccag gtgcccctgg
     4201 ccgggcgacg cgacctccag cagaaagcag tatccggcgc cgtccaaacc cggctcgccg
     4261 agggtttgca gcgggatttt cagggcgttg gcccccacgg tcccgatggg cgtgccgtgg
     4321 ggtgcgctca gctgccggcg gccggcctcg gcgtcgagca ggtagcgtcg tgagcagtcg
     4381 gggtcttcgt tgcggaagag gttcaattcg gcggtggagc cgctcgccca ggcgagtgtc
     4441 agaacggcat cgccgggcag cgatccgaga tcgtcgatct ccacatgcag gcgcgtgaag
     4501 tcttccaggt cccggctcga atcgggagcc ggggtgttgg cgtcg
//
LOCUS       DBNHQR010000150         4878 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_204508, whole genome shotgun sequence.
ACCESSION   DBNHQR010000150 DBNHQR010000000
VERSION     DBNHQR010000150.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4878)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4878
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_204508"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="AC16VR_03355"
     CDS             complement(<1..382)
                     /locus_tag="AC16VR_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003100230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HIR6010223.1"
                     /translation="MVMSSSPRLEVIVLAAGQGKRMNSRLPKVLHPLAGRPLLAHVLE
                     TVDELEPAGIHVVIGHGADLVREAIGAAALRWVVQTEQLGTGHAVQQALPDIDDSAVV
                     LVLCGDVPMVTAQTLRRCVTGAGNG"
     gene            complement(547..969)
                     /locus_tag="AC16VR_03360"
     CDS             complement(547..969)
                     /locus_tag="AC16VR_03360"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014873131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HIR6010224.1"
                     /translation="MAMTFHCDIVSAEREIFSGRVTMLSAIGTIGELGILPGHAPLLT
                     GIRPGPVRLRMDNGEEEVFFASGGYLEVQPGVVTVLADTALRAEDIDEAAAAAAQQDA
                     ERALREQTADIEFSAAAAMLAEATARQRTLAELRKRRR"
     gene            complement(981..2363)
                     /gene="atpD"
                     /locus_tag="AC16VR_03365"
     CDS             complement(981..2363)
                     /gene="atpD"
                     /locus_tag="AC16VR_03365"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008172594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIR6010225.1"
                     /translation="MSSGRIVQIIGAVIDVEFSREDVPKVYDALKVSSTGLTLEVQQQ
                     LGDGVVRAIAMGPSDGLSRGLEVTNTQQPISIPVGEETLGRIMNVLGEPIDEKGPVNA
                     QQKMPIHRKPPSYEDQKGGNEILETGVKVIDLICPFAKGGKVGLFGGAGVGKTVTMLE
                     LIRNIATEHSGLSVFAGVGERTREGNDFYHEMEESGVLDKISLVFGQMNEPPGNRLRV
                     ALTGLTLAEQFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPNLAEEMGV
                     LQERITTTKTGSITSVQAVYVPADDLTDPSPATTFAHLDSTVTLSRAITDLGIYPAVD
                     PLDSTSRQLDPLVVGEEHYQVARGVQQVLQRYQELRDIIAILGMDELSEEDKQLVYRA
                     RKMQQFFSQPMFVAEPFTGQPGVYVSREDTVRSFKGILEGEYDHLPEQAFYMVGTIEA
                     AIEKAETLSA"
     gene            complement(2395..3255)
                     /gene="atpG"
                     /locus_tag="AC16VR_03370"
     CDS             complement(2395..3255)
                     /gene="atpG"
                     /locus_tag="AC16VR_03370"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HIR6010226.1"
                     /translation="MAVGKEIKKKIGSVENTKKITSAMQMVAASKMRRTQERMREGKP
                     YSEKIRAVIGHLANSNPEYRHVYMEPREISRIGYLVVSTDKGLCGGLNINLFRTLIRD
                     MAEWHAKGKEIDLGLIGNKAAAFFRSVGGNIVAAMNNVGEDPALADLIGGIKVMLDAY
                     EEGKIDRLFIASNEFVNTMTQRPVVRQLLPLDPETDDSMRHRWDYIYEPDARQLIEGL
                     VTRFMESQVYQAVVENGACEQAAKMVAMKSATENAEKLIDELTLIYNNARQAAITQEI
                     AEIVGGAAAV"
     gene            complement(3266..4810)
                     /gene="atpA"
                     /locus_tag="AC16VR_03375"
     CDS             complement(3266..4810)
                     /gene="atpA"
                     /locus_tag="AC16VR_03375"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR6010227.1"
                     /translation="MQQLNPSEISDIIRQRIEGLDVRAQAQNEGTIVGVSDGIVRIHG
                     LADAQYGEMIEFDGGLYGMALNLERDSVGVVILGDYEGLSEGMTARCTGRILEVPVGR
                     ELLGRVVDSLGNPIDGKGPLNASETAPIEKVAPGVIARQSVDQPVQIGLKCIDSMVPI
                     GRGQRELIIGDRQIGKTAIAIDAIINQKDSGIRCVYVAIGQKMSTIANIVRTLEEHGA
                     MENTIVVAASASDPAPMQFIAPYSGCSMGEYFRDNGEDALIIYDDLTKQAWAYRQISL
                     LLRRPPGREAYPGDVFYLHSRLLERAARVNADYVEQYTGGKVKGRTGSLTALPIIETQ
                     AGDVSAFVPTNVISITDGQIFLETDNFNSGLRPAMSPGISVSRVGGSAQVPFIKKIAG
                     GIKLALAQYRELAAFSQFASDLDDATRRQLEHGRRVTELMKQKQYAPMSVAEMGVSLY
                     AANEGYLVDIAVDQVLEFEQGLLAYMNSEKADFMRRINETGDYDDGIVAEMRQGIEAY
                     KAQANF"
BASE COUNT          829 a         1594 c         1498 g          957 t
ORIGIN      
        1 cgccgttgcc ggcgccggtg acgcagcgcc tgagcgtctg ggccgtcacc atcggcacgt
       61 cgccgcacag aaccagaacc acggctgaat cgtcgatgtc cggcagggcc tgctgcacgg
      121 cgtggccggt gcccagctgc tcggtctgca ccacccaccg cagcgccgcc gcgccaatgg
      181 cctcacgcac cagatcggcg ccatgaccga tgaccacgtg aatgccggcg ggctctagct
      241 cgtcaacggt ctccaggacg tgggcgagca ggggacgccc ggccagcggg tgcagcactt
      301 tcggtaaccg cgagttcatg cgcttaccct ggccggcggc cagcacgatg acttcaaggc
      361 gagggctgga agacataacc atgttgagca gaaacagtcc ctggtttcag gccgggccga
      421 accgcacccg cgtcacgggc ggccgaacct ctatgctctg acgattatag aaccggggaa
      481 cgaataaagg gtgacacaga tcacccctgc attggacaag aaccttgggc agattcgccc
      541 ggcgcgtcag cgccggcgct tgcgaagctc tgccagcgtc ctctgccgcg cggtcgcctc
      601 ggcaagcatg gcagcggcag cggaaaattc gatatccgcc gtctgctccc gcaatgcccg
      661 ctccgcatcc tgctgagcgg cagcagcggc ggcctcgtcg atgtcttccg cgcgcagcgc
      721 cgtgtcggca agaacggtga ccacccctgg ctggacctcg agataccctc cggaggcgaa
      781 gaagacctct tcctcgccat tgtccatgcg cagccggacc ggccccggac ggatgcccgt
      841 cagcagcggg gcgtggccgg gcaggatgcc cagctcaccg atggtcccga tggcgctcag
      901 catggtcacc cggccggaga agatctcgcg ctccgcgctg acgatatcgc aatggaaggt
      961 catggccatg gccgctctcc ttaggcgcta agcgtctcgg ctttctcgat cgccgcttcg
     1021 atggtgccca ccatgtaaaa cgcctgctcc ggaaggtggt cgtattctcc ctcgagaatg
     1081 cccttgaagc tgcgcaccgt atcctcccgg gagacgtaca cgcccggctg gccggtaaag
     1141 ggctcggcga cgaacatggg ctgcgagaag aactgctgca tcttgcgcgc gcgatagacc
     1201 agctgcttat cctcctcgga aagctcgtcc atcccaagga tggcgatgat gtcgcggagc
     1261 tcctggtagc gctgaagcac ctgctgaacg ccccgggcca cctgatagtg ctcctcaccg
     1321 accaccagcg ggtcgagctg acggctcgtg gaatccagcg gatccacggc ggggtagatg
     1381 ccgaggtccg tgatggccct ggagagcgtc accgtggaat ccaggtgcgc aaaggtggtg
     1441 gccggagacg ggtcggtgag gtcatcggcg ggcacgtaaa cggcctgcac cgacgtgatc
     1501 gatccggtct tggtggtggt gatccgctcc tgcagcacgc ccatctcctc ggccaggttc
     1561 ggctggtagc ctaccgcgga cggcatccgg ccgagcagcg cggacacctc ggttcccgcc
     1621 agcgtgtagc ggtagatgtt gtccacgaag aggaggacgt cgcgaccttc gtcgcggaac
     1681 tgctcggcca gggtcaggcc ggtgagcgct acccgcagac ggttacccgg cggctcgttc
     1741 atctgcccga aaacgagcga gatcttgtcg agcacgcccg actcctccat ctcgtggtag
     1801 aagtcgtttc cttcccgggt ccgctcgccc acaccggcga agaccgacag gccggagtgc
     1861 tcggtggcga tgttgcggat cagctccagc atggtgaccg tcttgccgac gccggcaccg
     1921 ccgaacaggc ccaccttgcc gcccttggcg aatgggcaga tgaggtcgat gaccttgacg
     1981 cccgtctcga gaatctcgtt gccgcctttc tggtcctcgt atgagggcgg cttgcggtga
     2041 atgggcatct tctgctgcgc gttaactggc cccttctcgt cgatgggctc gccgagcacg
     2101 ttcatgatcc ggccaagggt ttcttcaccc acggggatgg agatgggctg ctgggtattg
     2161 gtcacctcca gaccccgcga gagaccgtcc gagggcccca tggcaatggc acgaaccacc
     2221 ccgtcaccga gctgctgctg cacctcgagg gtgagccccg ttgaggaaac cttaagggcg
     2281 tcgtagacct ttgggacatc ctcccgcgag aattcgacgt caatcaccgc gccgataatc
     2341 tgtacgattc gtccgctgct catgctttgt gcctcttcaa tctaatctgt ctatctaaac
     2401 cgccgcggcc ccgccgacga tctccgcaat ctcctgagtg atcgccgcct gccgagcgtt
     2461 gttgtaaatc agggtcagct cgtcgatgag cttctccgcg ttttccgtgg cgctcttcat
     2521 ggcgaccatc ttagccgcct gctcgcaggc accgttctcc accaccgcct ggtagacctg
     2581 ggattccatg aagcgggtaa ccaggccctc gatgagctgg cgcgcgtccg gctcgtagat
     2641 gtagtcccag cgatgcctca tgctgtcgtc ggtttccggg tccaggggca ggagctgccg
     2701 gaccaccggg cgctgggtca tcgtattgac gaactcgttg ctggcgatga acagccggtc
     2761 gatcttgcct tcctcgtaag cgtcgagcat gaccttgatc ccgccgatga ggtcggccag
     2821 cgcaggatcc tcgccgacgt tgttcatggc cgcgacgatg tttccaccaa ccgagcggaa
     2881 gaacgccgcc gccttgttgc cgatcaggcc aaggtcgatc tccttgccct tggcatgcca
     2941 ctccgccatg tcccggatca gggtgcggaa caggttgatg ttgaggccgc cgcagagtcc
     3001 cttgtccgtg gagaccacga ggtagccgat ccggctgatc tcgcgaggct ccatgtagac
     3061 gtgccggtac tccgggttgg agttcgccag atggccgatg accgcgcgga tcttttccga
     3121 gtagggcttg ccttcccgca tccgctcctg ggtgcggcgc atcttgctcg ccgcaaccat
     3181 ctgcatcgcg ctcgtaatct tcttggtgtt ctcgacgctg ccgatctttt tcttgatctc
     3241 tttgccgacc gccatatccg ctgccttaga agttcgcttg tgctttgtag gcttcgatgc
     3301 cctggcgcat ctccgcaacg atgccgtcgt cgtagtcgcc ggtctcgttg atgcgccgca
     3361 tgaagtcggc tttctcggag ttcatgtagg caagcaggcc ctgctcgaac tcgagcacct
     3421 gatcgaccgc gatgtccacc aggtagccct cgttcgccgc ataaaggctc acgcccattt
     3481 ccgctacgct catcggcgcg tactgcttct gcttcatgag ctccgtgact cgccggccgt
     3541 gctcgagctg gcgccgcgtc gcgtcgtcaa ggtctgaagc aaactgagag aacgctgcga
     3601 gttcccggta ctgggcaagg gcgagcttaa tgccaccggc aatcttcttt atgaagggca
     3661 cctgggcgga tccccccacc cgggataccg agatgcccgg gctcatggca gggcgcagcc
     3721 ccgagttgaa gttgtccgtc tctaagaaga tctgcccatc ggtgatggag atgacgttcg
     3781 tcggcacgaa cgcggatacg tcccccgcct gagtttcgat gatcggcagc gccgtcaggg
     3841 acccggtccg gcccttgacc ttaccccccg tgtactgctc cacgtaatcg gcgttcaccc
     3901 ttgccgcacg ctcgagcaaa cgcgagtgca gatagaagac gtcgccggga taagcctcgc
     3961 ggcccggcgg ccggcgcagc aggagcgaga tctgccggta ggcccaggcc tgtttcgtga
     4021 ggtcgtcgta aatgatcagg gcgtcttcac cgttatcccg gaagtattcg cccatgctgc
     4081 agccggagta aggcgcgatg aactgcatgg gcgccggatc ggatgcgcta gcagccacga
     4141 cgatggtgtt ctccatggcg ccgtgctctt cgagggtccg gacgatgttg gcaatcgtcg
     4201 acatcttctg cccgatggcc acgtagacac aacgaatgcc gctgtccttc tggttgatga
     4261 tggcgtcgat ggcgatggcc gtcttgccaa tctgcctgtc gccgatgatg agctcgcgct
     4321 gaccccggcc gatgggcacc atggagtcga tacacttcag cccgatctga acgggctggt
     4381 ccacggactg ccgcgcaatc acgccggggg caaccttttc gatgggcgcg gtttccgagg
     4441 cgttcagcgg ccctttgccg tcaatggggt tgccaaggga atccaccacc cggccgagca
     4501 gctcgcggcc caccgggact tcgagaatcc gcccggtgca tcgagccgtc atcccctcgg
     4561 agagaccctc gtagtcgccg aggatcacca cgccgacaga gtcgcgctcg aggttgagcg
     4621 ccattccgta gaggcccccg tcgaactcga tcatctcgcc atactgggcg tcggcaaggc
     4681 cgtggatgcg gacgatgcca tctgacacgc ccacaatggt gccttcgttc tgcgcctgcg
     4741 cgcgtacgtc caatccttca atccgctgcc tgatgatgtc gctgatctcg gaaggattca
     4801 gttgttgcat ttcgaattcc tcagtctctt tcttggagcc tcgacggtgt cgttcccgcc
     4861 gaaggcctct gtattcag
//
LOCUS       DBNHQR010000151         9388 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1217916, whole genome shotgun sequence.
ACCESSION   DBNHQR010000151 DBNHQR010000000
VERSION     DBNHQR010000151.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9388)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9388)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9388
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1217916"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..570)
                     /locus_tag="AC16VR_03380"
     CDS             complement(94..570)
                     /locus_tag="AC16VR_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6010228.1"
                     /translation="MNADNQKSRVLIVDDEPGNIKILSSVLAGEYALSVATNGEQALQ
                     IAKVQSPEIILLDMIMPGMDGIQVCEALKASEETKDIPVIFVTSMSDTANEERGLEAG
                     AVDYITKPISPPIVKARVKIHIQNYLSMQFLQKLLSDQTGSLDAAKEEAESLLVFV"
     gene            732..4061
                     /locus_tag="AC16VR_03385"
     CDS             732..4061
                     /locus_tag="AC16VR_03385"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HIR6010229.1"
                     /translation="MLFVIVGSLALVLAMLYTSPVAWGLAILIDGILLPLSYLSYRWA
                     KQDRRETSATVLAVIWYAIAVGMIIVGERLYGILLVTSILPVLMSLPYVSQVLLRRLI
                     LTSVLLVLVGSVGALFPPIITPTVPDDLIAYVESFSTASITMMVMLAIWQSAGRLRAS
                     ADGMREAIAALKESERGLETKVEARTAELRKAVKEMADLNEIAIIVNSTLDLDKVKDS
                     IYQGLQNLFAFEQMGVFLADYDEEKLYLRLQAGAPFDPELSRILVEEGLPLDSSHSFA
                     ARSVVKNRSVFLGAITDEGLAMAGVGDRLIYEHNPMKSILMCPLEIQNRAIGCIYFSA
                     SREAFELHQNDIESIERYVTQLGTALRNAQLFQEAEEARAEAEAANETKGTFLANMSH
                     EIRTPMNAIIGLTGLCLETELDDKQEDYLRKVDGAANALRSIIDDILDFSKLEAGKFE
                     FEDIPFSLNDVLDNLATICMVRCQDKHLELVFQRDPGLPDALSGDPTRLGQILINLAG
                     NAIKFTEEGQIVIEARETGRVGDRVSIRFDVRDSGIGMNEEQLSRLFQSFSQADATIS
                     RQYGGTGLGLAISQQLSEGLGGHIEVTSEPGVGSSFHFTLEFAVVEAREEGAEREDAP
                     RNLNVLVVDDNEPSRDILREYLESFGYTVTLAESGEEGLALMKPAHDFDLVLLDWMMP
                     GMTGLDVALAIREQPSPPKVILLSSWNMPSTEHQSMVDAFMAKPVKPSTLLDTIMLAY
                     GKQVVRRKRGLGRGMGHEDLAPVRGARVLVVDDSDINLQIACELLQKVPFVLDTASDG
                     AQAVDKVLAHHYDCVLMDIQMPGMDGYTATGIIRERFSFDELPILAMTANVMAEDRAR
                     TRDAGMNGHVAKPVDPAELFQALAEAIPVADYSANLPEAEDATVVEAAAAPQNEPLPD
                     SLPGLELKQGLARLGGNEDLLLTLLRNLLDEYADCPSDLQRLLETGDEEEARKVAHKL
                     RGIANNLGATGIGKCAEDIERCVLDGRRVDPVHVADLQEAQQVLATQLAELLASRTTG
                     GGVGNAAEDVDVGAVLSALKSAIAAFDPGAVELVDQLLAGCDPQSNVVQLLEASRQHL
                     DNFNFTDAEPLVAEAEAALLA"
     gene            4094..7108
                     /locus_tag="AC16VR_03390"
     CDS             4094..7108
                     /locus_tag="AC16VR_03390"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF019315.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HIR6010230.1"
                     /translation="MLVLLSAGNGLAASLEFDDLGEPRSITGEWLYMTGDDPGWADPG
                     LEDGGWLPVTVPGPGIGDHPDAQGMVWYRVHVRFGPPIAADLDARAALGVAIGSVESA
                     YEIYAGGRLLGGMGKLPPEPVAHFDQHGIYPIPLDAVDADGNLVLALRVWRHERAASR
                     SELGHFAGPFRVGSIGDLRAEAVLAAIIPNVLLAGVYLMVGLYHLFVARRNPSMREFF
                     WFGWMALILAAYSVETSQWKYTIDLPFLVHKKIEYVVLYLGPVVMTETFARIAAVRLN
                     IFWRVIQAVFVGYAALVLLIPNIDIHYLTLTSFQYLAAIWTLGIVGLMGWHAFKGNRE
                     ARSISGLMILFFFAVLTDVILDIKLLGANNLLHLMFALVVLLMAVLMANRYTATLAKL
                     ERTVEDHTADLREANLRLERASAAKSQFLANMSHELRTPMNAIIGLTHLGLKTELSDQ
                     QREYLTTVDHSAQGLLGIIESILDFSDLDGGEVTLDEQVFNIADVLERQAAIAGPAAQ
                     EKGLDLQFHGAANLPVALVGDPRRLGQVLAVFIGNAIKFTDRGSIRVEVGLASATDDT
                     ASLEFSVTDSGPGMSAEEQKHLFDPFSQADESHTRTHGGTGLGLAVASKLAALMDARI
                     DVSSEPGRGSTFSLVLTLPLATDDAGDGPDSGTDAVLDLGPIRGARVLLVDDSEINLQ
                     VASEILRQIPVRVEVAHDGQEAVTMTESHHYDCVLMDVQMPVMDGYAATEAIRAGPLG
                     EDLPILAMTANVAPEDQARAKAAGMNAHIAKPIDPDRLFRELLTWIEPGEREPIEPEL
                     AAEPASLAELPDELPGIRVGEGLARVGGNVDLYVSLLKDLARDYADCHTQLGELLGAG
                     ATQEAAALAHKLKGIANNLGVVGVGTQAAALESALRSAEPVSESALQNLAEALAESST
                     AIEQLVALQSAAEGGGEESGGNSLELLDRLEKEIGENDPAASDTAEELLKSLEADSAA
                     VTAATAARDALELFDFAGAGEQLDQIRAGLGS"
     gene            complement(7160..7954)
                     /locus_tag="AC16VR_03395"
     CDS             complement(7160..7954)
                     /locus_tag="AC16VR_03395"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR6010231.1"
                     /translation="MTDLTTYYRDHWLDVEDERMARYEAMFAWREGQEVLIAPAAIGA
                     GQLVVDYGCGPGGLAVELARRVGSTGKVIGLDINAEFLERARARAADEGVAERVETRL
                     VTGDRFPMDTHAVDRVICKNVLEYVPDPQATINEFHRILRPGGIAHVSDSDWGAVVFE
                     PAREPFARIMSAAAVAFRTPLIGRSLYGMFRRAGFDDVRVQVLAAADTTGALRGVLHN
                     MAGYALTSGQVDRADVDAFISDIDRALDDKTYLAVLPQFLVTGRVP"
     gene            complement(7994..9205)
                     /locus_tag="AC16VR_03400"
     CDS             complement(7994..9205)
                     /locus_tag="AC16VR_03400"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR6010232.1"
                     /translation="MATLDILPTQIDFSALHARMQWYVDERIIPHCVCLVMRGTDVLD
                     YRTLGYMDLESREPLREDAIFRMYSNTKIVTSVAAMMLFEEKRFELDDPVARFIPSFS
                     DSQVLRPGAKSAKDVEPARSPITMRQILSHSAGLSYGVFEPTSVVDQLYAQAGLHLLS
                     DTDGTLENLCEVVSRLPLAFQPGTNWRYSVSTDVTARVIEVISGQRFDVFLKERILDP
                     LGMADTDFHVPAEKQHRFTTMYAPVDLFAPMKGGLNKADDPLSGMYSRPHAMLAGGHG
                     LVSTVADYVTFLRALINGGEWHGVRLLQAETLELMRTNQLADGVSVHFPMWEMKDNVF
                     GLGFALKQAPADGEPETAVGEYHWGGIAGTHSWMAPRAGIAGVCMTQRMPGFWHPFSQ
                     EFKRMVYEQLG"
BASE COUNT         1676 a         2903 c         3043 g         1766 t
ORIGIN      
        1 agcagttctg ggccaagacc aagacgggct atcgggggag cttcgtcgac tacatcgacg
       61 agctgatggt cggctagtcg ggctgaactg cggtcaaacg aagacgagca gcgactcggc
      121 ttcctccttg gccgcgtcga gggagccggt ctggtccgac agcagtttct gcagaaactg
      181 catgctcagg taattctgta tgtggatctt cacccgggcc ttgacgatgg gcgggctgat
      241 cggcttggtg atgtagtcga ccgcgccggc ttccagcccg cgctcctcgt ttgccgtatc
      301 gctcatgctg gtgacgaaaa tcaccgggat atccttggtc tcctcgctcg ccttgagagc
      361 ttcgcagacc tggatgccgt ccatgcccgg catgatcatg tccagcagga tgatctccgg
      421 tgactgaacc ttggcgatct gcagggcctg ctcgccgttc gtggcgacgc tcagggcgta
      481 ctcgccggcc agcacgctgg atagaatttt gatgttgccg ggttcatcgt ccacgatgag
      541 cactcggctt ttctgattgt ctgcgttcat ccccgactcc agattgccag cgccgaagct
      601 atcacttctc gattatactg gcgagccgag atcgataaag acgacgaccg atagggcctt
      661 ccgtgaacga tgattccacc gcgctgagtg tcgaagcgcg ctcctttacg cgcataaccg
      721 ttattagcgg gttgctgttc gtcattgtcg ggtccctcgc cctggttctc gcgatgctgt
      781 atacgagccc ggtggcctgg gggctggcga ttttgatcga cggcatcctg ctgccgttgt
      841 cgtatctcag ctatcgctgg gcgaagcagg accggcggga gacgtcggcg acggtactcg
      901 ccgtcatctg gtacgcgatt gcggtgggca tgatcatcgt cggggaacgc ctctacggca
      961 tcctgctggt gacttccatc ctgccggttc tcatgagcct gccctatgtt tcgcaggtgc
     1021 tgctgcgccg gctgatcctc acctcggtgc tcctggtgct ggtgggcagc gtgggcgctc
     1081 tgtttccgcc gataatcacg cccacggtgc ccgatgacct catcgcctac gtggagtcct
     1141 tttccacggc aagcatcacg atgatggtga tgctggcaat ctggcagtcc gccgggcgcc
     1201 ttcgtgcttc cgccgacggc atgcgagaag cgatcgccgc gctcaaggag tcggagaggg
     1261 gcctggagac caaagttgaa gcccgcacgg ccgagttgcg gaaggcggtc aaggaaatgg
     1321 ctgatctcaa cgagatcgcg atcatcgtca actcgaccct cgacctggac aaggtcaaag
     1381 acagcatcta ccagggcctg cagaacctgt ttgccttcga gcagatgggc gttttcctgg
     1441 cggactacga tgaggaaaag ctgtacctgc gcttgcaggc gggtgccccg tttgatcccg
     1501 agctgtctcg gatccttgtc gaggaaggcc tgccgctcga ttcgagtcac agctttgcag
     1561 cccgatccgt ggtgaagaac aggtcggtct ttctcggcgc gataactgac gagggcctgg
     1621 ccatggctgg tgttggggat cggctcatct acgagcacaa ccccatgaag tccatcctga
     1681 tgtgtcccct cgagatccag aaccgggcca tcggctgtat ctacttctcc gccagcagag
     1741 aagcgttcga gctgcaccag aacgacatcg agtccatcga gcgctacgtg acccagctgg
     1801 gcacggctct gcgcaacgcc cagctctttc aggaagccga agaagcccgc gctgaggccg
     1861 aggccgccaa cgagaccaag gggacattcc tggccaacat gagccacgaa atccgcacgc
     1921 ccatgaacgc catcatcggc ctgaccggtc tctgcctgga aaccgagctc gacgacaagc
     1981 aggaggacta cctgagaaag gtcgacggtg cggcaaacgc cctgcgcagc atcatcgacg
     2041 acattctcga cttctccaag ctggaagcgg gcaagttcga gttcgaggac attcccttct
     2101 cgctcaacga cgtgctcgac aacctcgcca ccatctgcat ggtgcgctgc caggacaagc
     2161 atctggagct cgtcttccag cgcgatcccg ggttgcccga cgctttgtcg ggtgatccga
     2221 cccgtctcgg tcagattctc atcaacctgg ccggcaatgc catcaagttc accgaggagg
     2281 gccagatcgt catcgaggcc cgcgagaccg gccgcgttgg cgacagggtg agcattcgct
     2341 tcgacgtgcg tgacagcggc atcggcatga acgaggagca gctgagcagg ctgttccagt
     2401 ctttttcaca ggccgatgcc actatcagtc gtcagtatgg cggcactggc cttggccttg
     2461 ccatctcgca gcagctcagc gaaggccttg gcggtcacat cgaggtcacg agcgagcccg
     2521 gtgtcggcag cagctttcat ttcactctcg agttcgccgt cgtcgaggcg cgcgaagaag
     2581 gggccgagcg ggaggatgcg cccaggaacc tcaacgtgct ggtggtggat gacaacgagc
     2641 cgtcccggga cattctaaga gagtatctgg aatcgttcgg ctacacggtg accttggctg
     2701 agagcggaga agagggcctc gccctcatga aacccgcgca cgacttcgat ctggtgttgc
     2761 tggactggat gatgcctggc atgacgggcc tggacgtggc cctcgcgatc cgcgagcagc
     2821 cgagcccgcc aaaggtcatc ctgctgtcgt cctggaacat gccgagtacc gagcatcagt
     2881 ccatggttga tgcgttcatg gcgaaaccgg tgaaaccgtc gaccctgctc gacaccatca
     2941 tgctcgccta cggcaagcag gtggtaaggc gcaaacgcgg cctgggtcgg gggatgggcc
     3001 atgaagacct ggctcccgtt cgcggcgcac gggtgctcgt ggtggatgat tctgacatca
     3061 acctccagat tgcctgcgaa ctgctgcaga aggtgccctt cgtgctcgat accgccagcg
     3121 atggcgcgca ggcggtggac aaggtgctgg cccaccacta cgactgcgtg ctcatggata
     3181 tccagatgcc cggaatggac ggctacaccg ccacgggcat cattcgcgag cgtttctcgt
     3241 tcgacgagct gccgatcctc gccatgaccg ccaacgtgat ggccgaggac cgggcgcgca
     3301 cccgcgatgc cggcatgaat ggccatgtgg ccaagccagt ggaccctgcc gagttgttcc
     3361 aggcgctggc cgaggccatc ccggtagccg attactccgc caaccttccc gaggcagagg
     3421 acgccactgt cgtggaggcg gcggctgctc cgcagaacga gccgctgccg gattccctgc
     3481 ccggactcga gctcaagcaa ggacttgccc ggcttggagg caacgaagac ctgctgctga
     3541 cgctcctgcg gaacctgctg gatgagtacg ccgattgccc ttccgacctg cagcgtttgc
     3601 tggaaaccgg agatgaagaa gaggcgcgca aggtcgcaca taagctcagg ggcatagcaa
     3661 ataacctcgg ggccacgggc atcgggaaat gcgccgagga catcgagcgc tgtgtcctcg
     3721 acgggcggcg ggtggatccg gtccatgtgg cggacctcca ggaagcccag caggtcctgg
     3781 cgacgcaact cgctgagctg ctggcgtcgc gaacgacggg cggtggcgtg gggaacgcag
     3841 ccgaagatgt cgacgttggg gccgtgctgt ccgccctgaa gtccgcaatt gccgctttcg
     3901 accccggtgc tgtagagctc gtcgatcagc tgctcgcggg atgcgacccg cagagtaacg
     3961 tcgtgcagct gctcgaagcg tcccgtcagc acctcgacaa cttcaacttc actgacgcgg
     4021 agccgctggt ggcggaagcg gaggcggccc tgttggcctg atcatgaagc tgcctcgacg
     4081 gtgctgcgcc ctcgtgcttg ttttgctctc cgccggcaac ggtctggccg cttcgctgga
     4141 attcgacgat ctcggtgagc cgcgctcgat aaccggtgag tggctttaca tgacgggcga
     4201 tgaccctggt tgggctgatc cgggcctgga ggacggcggg tggctgccgg tgaccgtgcc
     4261 aggtcctgga atcggcgacc accccgacgc gcagggaatg gtctggtacc gggtgcacgt
     4321 ccgtttcggt ccgccgattg ccgccgatct cgacgcccgc gcggcgctcg gggtcgccat
     4381 tggcagcgtg gagagcgcct acgagatata cgccggcggg cgtctgcttg gtggcatggg
     4441 caagctgccg ccggaaccgg tcgcgcactt cgatcagcac gggatttacc cgattccctt
     4501 ggatgccgtc gatgccgacg gaaatctggt gctcgctctg cgcgtctggc gccatgagag
     4561 ggccgcctcg agatcagaac tcgggcactt cgccgggccg ttccgcgtcg gcagtatcgg
     4621 agaccttcgc gccgaggcgg tgttggcagc gatcattccc aacgttctgc tggcgggcgt
     4681 ctacctgatg gtcggcctgt atcacctgtt cgttgcccgg cggaacccga gcatgcggga
     4741 gttcttctgg ttcggatgga tggcgctgat actcgcggcg tactcggtcg agaccagcca
     4801 gtggaagtac acgatcgatc ttcccttcct ggtgcacaaa aagatcgagt acgtggtgct
     4861 ctatctggga ccggtggtca tgaccgagac gttcgctcgg atcgccgctg tacgtctcaa
     4921 catcttctgg cgcgtgattc aggccgtatt tgttggatac gcggcgctcg tgctgctgat
     4981 tcccaacatc gacatccact acctgacgct gacctccttt cagtacctgg ccgcgatctg
     5041 gacgctcgga atcgtcggcc tgatggggtg gcacgcgttc aagggcaatc gggaagcccg
     5101 ttccatatcg ggcttgatga tcctgttttt ctttgccgtg ctcaccgacg tcatcctgga
     5161 catcaagctg ctcggtgcga acaacctgct ccacctcatg tttgccctgg tcgtcctcct
     5221 gatggcggtg ctgatggcga accggtacac ggccacgctg gcaaagctgg aacgaaccgt
     5281 agaagaccat accgccgacc tgcgggaggc caatctgcgc ttggagcggg cgtcggcggc
     5341 caaaagccag ttcctcgcca acatgagcca cgagttgcgt acccccatga acgccatcat
     5401 cggactcacc cacctggggc tgaagaccga gctttcagac cagcagcgcg agtatctaac
     5461 cacggtcgat cattccgctc agggtctgct cggcatcatc gagagcattc tcgatttctc
     5521 ggatctggac ggcggcgagg taacgctcga tgagcaggtg ttcaacatag ccgacgtgct
     5581 ggagcggcag gcggccatcg ctggccctgc ggcgcaggaa aaggggctcg accttcagtt
     5641 tcatggggcg gcgaaccttc ccgttgcgct ggtgggcgac ccccggcggc tgggccaggt
     5701 gctggccgtc ttcatcggca atgccatcaa gtttacggat cggggcagca tccgtgttga
     5761 ggtcggcctt gcgtccgcaa ccgacgacac cgccagcctt gaattcagcg tcacggactc
     5821 ggggccgggc atgagcgccg aggagcaaaa gcatctgttc gatccgtttt cccaggcgga
     5881 cgagagccat acgcggaccc acggaggcac agggttaggt cttgccgtcg cgtcgaagct
     5941 agcggcgctc atggatgcgc gcatcgacgt cagcagcgaa ccgggccggg gcagcacgtt
     6001 ttcgcttgtg ctgacgctgc ccctggcaac tgacgatgct ggtgacgggc cggattcggg
     6061 taccgacgcg gtgctcgatc tgggcccgat tcggggcgcc cgggtgctcc tcgtggatga
     6121 ctcggagatc aaccttcagg tggcctcgga gattcttcgc cagattccgg ttcgggtgga
     6181 ggtggcccac gacggccagg aagccgtgac catgaccgag agccaccatt acgactgcgt
     6241 gctgatggac gtgcagatgc cggtgatgga cggttacgcc gccacggaag cgatccgtgc
     6301 cgggccgctc ggcgaggatc tgccgatact ggccatgacg gccaacgtcg cacccgagga
     6361 ccaggccagg gcgaaagccg ccgggatgaa cgcgcacatc gcgaaaccca tcgatcccga
     6421 caggcttttc cgggagctgc tgacctggat cgagccgggg gaaagggaac ccattgagcc
     6481 ggagctggcc gccgagcccg cctccctcgc tgagctcccg gacgagttgc ccggcattcg
     6541 ggtcggcgaa ggccttgccc gcgtcggagg gaacgtcgat ctgtacgtct cgctgttgaa
     6601 agatctcgcc agagactatg ccgactgcca cacgcagctc ggcgagctgc ttggcgctgg
     6661 cgcaacgcag gaagcggccg cgctcgccca caagctcaag ggcatcgcca acaacctcgg
     6721 tgtggtaggc gtcgggaccc aggcagcggc gttggagtct gccctgcgat ccgccgagcc
     6781 cgtatcggaa tcagcgctgc agaatctggc tgaagcgctg gcagaatcct ccacggccat
     6841 cgagcaactc gtggcattgc agtccgcggc ggaaggcggt ggcgaagagt ctggcgggaa
     6901 ttcgctggag ctcctggacc gcctcgaaaa ggaaattggc gagaacgatc cggcagcctc
     6961 tgacaccgcc gaggaactcc tcaagagcct cgaggcggac tctgccgcgg tcacggcggc
     7021 caccgccgct cgggatgccc tggagctgtt cgactttgcc ggtgccggcg agcagctgga
     7081 ccagattcgc gcgggtcttg gatcctgaat tcttagcggc ctcaccggct gtgctggcag
     7141 cccggccact cgccgccggt tacggaacgc gaccggttac gagaaactgg ggcaatacgg
     7201 ccaggtaggt tttgtcgtcc agcgcgcggt cgatgtcgga gatgaacgca tcgacgtccg
     7261 ctctgtcgac ctggccggat gtgagcgcgt aaccggccat gttgtgcaga acgccgcgca
     7321 gggcgccggt cgtgtcggcg gcggcgagga cctgaacgcg cacatcgtcg aatccggcgc
     7381 ggcggaacat gccgtacagg ctgcggccga tcaggggtgt ccgaaaagcc accgcggctg
     7441 cggacatgat ccgggcgaac ggctcgcggg ccggttcgaa gaccactgca ccccagtcgc
     7501 tgtcgctgac gtgggcaatg ccgccgggcc gcagaattcg gtggaactcg ttgatggtgg
     7561 cctgtggatc cggcacgtat tcgagcacgt tcttgcagat cacccgatcg accgcgtgcg
     7621 tgtccatggg gaacctgtcg cccgtgacga gccgggtctc gacgcgctct gctaccccct
     7681 cgtctgccgc ccgggcccgg gcgcgctcga ggaattcggc attgatgtcg agcccgatga
     7741 ccttgcccgt gcttcccacc cgacgcgcca gctctacggc tagccccccg ggaccacatc
     7801 cgtagtccac gacgagctgg cccgcgccga tagcggccgg agcgatcaga acttcctggc
     7861 cctcgcgcca ggcgaacatg gcctcatagc gcgccatgcg ctcgtcttcg acgtcgagcc
     7921 agtggtcgcg gtagtaggtt gtcagatctg tcatggagga aatcccatca ggtgagcggc
     7981 aattgggtcc ttatcatccg agttgctcgt aaaccatccg cttgaattcc tgactgaagg
     8041 ggtgccagaa gccgggcatt cgctgggtca tgcagacgcc ggctatgccg gcccgcggcg
     8101 ccatccagga gtgggttccc gcgatgcctc cccagtgata ctcgccgacc gcagtctccg
     8161 gttcgccgtc ggcgggcgcc tgcttgagtg caaacccgag cccgaagacg ttgtctttca
     8221 tttcccacat ggggaagtgc acgctcaccc cgtcagccag ctggttggtc cgcatcagct
     8281 cgagggtttc ggcctggagc agccgcacgc cgtgccactc cccgccgttg atcaacgccc
     8341 gcagaaacgt cacgtagtcc gcgacggtgg agaccaggcc gtggccgccg gccagcatgg
     8401 cgtggggtct ggagtacatg ccgctcagcg gatcgtcggc tttgttcagc cctcctttca
     8461 tgggcgcgaa caggtcgacg ggcgcataca tggtcgtaaa ccgatgctgc ttctcggccg
     8521 gcacgtggaa gtcggtatcc gccattccca ggggatccag aatgcgctct ttgagaaaaa
     8581 catcgaaacg ctggccggag atcacttcga tgaccctggc ggtgacgtcg gtgctgacgc
     8641 tgtagcgcca gttggtcccg ggttgaaagg cgagcggaag ccggctgacc acctcacaga
     8701 ggttctcgag cgtcccgtcg gtgtcgctaa ggaggtgcag acctgcctgg gcgtagagct
     8761 gatcaactac cgaagtcggc tcgaagacgc cgtaggaaag accggccgaa tggctcagga
     8821 tctgccgcat ggtgatgggg gatcgggcgg gctcgacgtc tttcgcggat ttcgcccccg
     8881 gccgcagcac ctgcgagtcg gagaacgacg gtatgaagcg ggcaaccggg tcgtccagct
     8941 cgaagcgttt ctcctcgaag agcatcatgg ccgccacgga ggtgacgatc ttggtgttgg
     9001 agtacatgcg gaaaatcgcg tcctcgcgca ggggctcgcg ggattccaga tccatgtagc
     9061 cgagcgtgcg gtagtcgagc acgtcggtgc cccgcatcac gaggcagacg cagtgaggga
     9121 tgatccgctc atcgacgtac cactgcatcc gggcgtgcag ggcagagaag tcgatctgcg
     9181 tcggcaggat gtcgagcgtt gccatgatgt cgctcccggg cgcggccagc ggacgattct
     9241 accgccagcc gtcccagaat ggcgtgtgtt gggtggccgg gcagaatggt acgtgcaagt
     9301 accgtcccgg acgttccggg aactcggcca ttactctcga tgcggcgtcc gcggcagcgg
     9361 tattcgtcac cgagcttgaa gccgccca
//
LOCUS       DBNHQR010000152         1792 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_204635, whole genome shotgun sequence.
ACCESSION   DBNHQR010000152 DBNHQR010000000
VERSION     DBNHQR010000152.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_204635"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..1127
                     /locus_tag="AC16VR_03405"
     CDS             96..1127
                     /locus_tag="AC16VR_03405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIR6010233.1"
                     /translation="MAEHASNEGVNLQLHLKRVPEGLAGPGDFEVRETEIPRPGDGEV
                     LVRAHYFGLDAALRLIVRDSKDFLFRVEPGDLVRGSMAGEVIESNNPDYSVGDLVLAS
                     SGVQNYAISDGSDLERCDTSQAPLGSWLGGFGVSGLTAYFAITEECKPQPGQTVVVNG
                     AAGAVGSIAGQIARLAGARTIGITSSDEKCRWLTEELKYDHAINYKAGNLYDQLAEAA
                     PERIDVIFDNVGGDILDESLRLIGMRGVVLLCGSTSQYAAEHMTGPDNYIWLGTMRAR
                     MQGFVVFDYADRYEEARRRMARWIKDGQLKLPEYVVDGDIGDYPEAFLQLYQGANRGK
                     MLLRLPAAR"
     gene            complement(1186..>1792)
                     /locus_tag="AC16VR_03410"
     CDS             complement(1186..>1792)
                     /locus_tag="AC16VR_03410"
                     /inference="COORDINATES: protein motif:HMM:NF018038.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HIR6010234.1"
                     /translation="DTGIPPDDYRRSIEALIDGGTGIVMLNHGTVSWPLWPLWRSISG
                     SSFMLTEGELNGQRVPGSGYRGGHGPYPNPTISLTPVDPSHPVAAGLGNGFTVTDEIY
                     LKTATLGPKVTPLFESDYEFVAANFTPPPLAPPEEQAAWSHPEGSNVVVWANAAGNSP
                     VVAADIGDSPEAFGNADFRRLLRNALQWVASPEARQWVANR"
BASE COUNT          328 a          584 c          568 g          312 t
ORIGIN      
        1 atcagccaac gtcatcgcgc tcccctcggc ttttccaagc ttttcatccc cgacccgaac
       61 tgaagtagga tacagttttt cggggagggt tcaccatggc ggagcatgcc agcaacgaag
      121 gcgtcaatct tcaactccat ctgaagcggg tcccggaggg tctcgctggt ccgggggact
      181 tcgaagtccg cgaaaccgaa attccccggc ccggggacgg tgaggtgctg gtcagggctc
      241 actacttcgg gctcgatgcc gcgctgcgac tcattgtccg cgacagcaag gatttcctgt
      301 tccgggtcga gccgggtgac ctcgtgcgtg gctccatggc cggagaagta atcgagtcga
      361 acaaccctga ctactccgtc ggcgatctgg tcctggcgtc ctccggcgtc cagaactacg
      421 ccatcagcga cggcagcgac ctggaaagat gcgacacgag ccaggctccg ctcggatcgt
      481 ggcttggcgg gtttggcgtc tccggcctga ccgcctactt cgcgatcacc gaagagtgca
      541 aaccccagcc aggccagacg gtggtcgtga acggggcggc cggcgccgtt ggctccatcg
      601 ccgggcagat cgcgcggcta gccggcgcgc ggaccatcgg tatcacgagc agcgacgaga
      661 agtgccgctg gctaaccgaa gaactgaaat acgatcacgc catcaactac aaggccggta
      721 acctctacga ccagctggcc gaggctgcac ccgaacggat cgacgtgatc ttcgacaacg
      781 tgggcggcga tatcctcgac gagagcctgc ggctgatcgg catgcggggc gtggtgctcc
      841 tgtgtggctc cacctctcag tacgccgccg agcacatgac cggcccggac aattacatat
      901 ggctgggtac catgcgggcg cgaatgcagg gattcgtggt tttcgactac gcggaccgct
      961 acgaggaagc ccgccgcagg atggcgcgct ggatcaaaga tggccagctg aagctgccgg
     1021 agtacgtcgt cgacggcgat atcggcgact atccggaagc ctttctgcag ctctatcagg
     1081 gcgcgaaccg gggcaaaatg cttctgcggc tgccggccgc ccgctgactt tcgcccccag
     1141 caccgggccc gaacatgccc cgggcgacga tccggttttg atctcctacc ggttcgccac
     1201 ccactgccgg gcttccggcg acgccaccca ctgcagggcg ttgcgcagca ggcgacgaaa
     1261 atcggcgttg ccgaaagcct ccgggctgtc accgatgtcg gccgccacca ccgggctgtt
     1321 tccggccgcg tttgcccaga ccacgacgtt actcccttcc gggtgcgacc acgcagcctg
     1381 ctcctctggc ggggcgaggg gcggcggcgt gaagttagcc gcgacgaact cgtagtcgct
     1441 ttcgaacagc ggcgtcacct tcggaccaag cgtagccgtc ttgaggtaaa tctcgtccgt
     1501 cacggtgaag ccgtttccca ggcccgcagc cacgggatga gaaggatcga cgggcgtcag
     1561 gctgatggtc gggttgggat acggaccatg accgccccgg tacccggacc ccggcacgcg
     1621 ctggccgttc agctcacctt cggtcagcat gaaagaagac cccgatatgg atcgccacaa
     1681 gggccacagc ggccatgaga cggtcccgtg attgagcatc acgatgcccg ttccgccatc
     1741 gatcagtgcc tcgatgctgc gtcggtagtc gtccggcgga atgcccgtgt cc
//
LOCUS       DBNHQR010000153         3619 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1282207, whole genome shotgun sequence.
ACCESSION   DBNHQR010000153 DBNHQR010000000
VERSION     DBNHQR010000153.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1282207"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            263..706
                     /locus_tag="AC16VR_03415"
     CDS             263..706
                     /locus_tag="AC16VR_03415"
                     /inference="COORDINATES: protein motif:HMM:NF025691.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HIR6010235.1"
                     /translation="MEEVMKQRLTSRNLWRRVLYMLLFVLAYGIAEAVVAVVVVVQFF
                     FILISGRANENLLVFGNNLSTYVRQILQFQTFNSEYLAYPFGDWPDEGLGSELWLEEG
                     TAEEAEAAPPVAGSEPPDEAEPDSGVAERGPIEGSADPEEPDPRG"
     gene            complement(723..1055)
                     /locus_tag="AC16VR_03420"
     CDS             complement(723..1055)
                     /locus_tag="AC16VR_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010236.1"
                     /translation="MNQRVVAGILALALCLHALPASARLYSYVNEEGDYVVSQKKPKN
                     VDEYAVLTDEGEFIRLVKRKRDDVPITHWRPWYMPNEPDPINPVEPGLEQRTPEVLIE
                     EVDGPEPN"
     gene            complement(1110..>3619)
                     /locus_tag="AC16VR_03425"
     CDS             complement(1110..>3619)
                     /locus_tag="AC16VR_03425"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6010237.1"
                     /translation="GDQDILAVTRAVKSYLDDADERLPQGVDLTIWNDNSEFMRDRLD
                     TLMNSARQGFMLVLLMLALFLRPRLAFWVSVGVPAAFLGAIFVANLAGLSIDGISTFG
                     FILVLGILVDDAVVIGERVHSLQSRAGESPPSETLISAAIEGTRQVSMPVIFGVLTTI
                     TAFLPLTFGPGPTGQIQSVISVIVLCCLTFSLIESQLVLPAHLGHRRVQTAGGEVGLM
                     MVPLVAIVLMEFAWDLRSFFAIGIGTTAVLIAWSIQGGFERFAARVIALQKQFSDRLE
                     HVIQFRFRRLVSAATDARYVTAAGAVAILGITVGLVAGGHMPFTYFPSLPADRISAKL
                     TMPLGTPARVTEEIVTNIAVTAEVLQRELASEQPDQPPVRFILAAVGAHPVSSAGLGA
                     VIAPPAARTGGHMGEVTLQLTPSDERDLSTLEIANRWRDATGPIPDAVEFTFATSTFS
                     VGSEIDIQLAGPDLEELANVAAEIRSELAAYPGVIDITDSFRSGKRELKLDILPAGEA
                     LGLNLGALARQVRQAFYGEEIQRIQRGRDDVRVMLRYTEAERRALGSLDEMRIRTRNG
                     SEVPFATVASADLGRGFSAIKRTDGRRVVNVTADVDRNVITANEVLADFGPGPLRTIL
                     RDHPRVSFSLEGTRREQEDSIASLLPMFLMALAVIYALLAVPLRSYTQPLIIMSVIPF
                     AMAGAVWGHMIMKSLGLLSNIVIMSVTGGIAACGVVVNSSLVLVHGVNARRREGMDIH
                     SAVVEAAVTRCRPILLTSLTTFAGLSPLLLNQSVQAQILIPMATSVAWGVVFATVVTL
                     LVVPVGYLILDDLGRKLSRGRTSVSPSSADAGASAGR"
BASE COUNT          654 a         1141 c         1207 g          617 t
ORIGIN      
        1 gaggccgagg ccgccgcggc ttgagtcgcc ggaacgcccc cgcgcccgcc tgtggggtgt
       61 gcggcttcgc ctcaatgtgg tgaatctcgc catcttcgag gtgggcctgt gagggcaacc
      121 atctgaaaat catgggaaaa aagaattggt accaatcttg cgacactgcc tggcaacaga
      181 gggccccaag cggcccgggc gaactcgccc ctgtgtccgt cgaaccctca gccgtgatgt
      241 gttggcaaat caaggaggcg ccatggaaga agtgatgaag caacggctca cgagccgaaa
      301 cctctggagg cgcgtccttt acatgctgct cttcgtgctt gcctacggca ttgccgaggc
      361 ggtcgtggcg gtggtggtgg tcgtgcagtt cttcttcatc ctgatctcgg gtcgggccaa
      421 cgagaacctg ctcgttttcg ggaacaatct cagcacctat gtccgccaga tcctgcagtt
      481 tcagaccttc aacagcgagt atctggctta tccgttcggg gactggccgg acgagggtct
      541 gggtagcgag ctctggctcg aggagggcac ggccgaggag gcggaggccg cgcccccggt
      601 ggccgggtca gagcctccgg acgaagcgga gcccgactcc ggcgtggccg aacgcggtcc
      661 gatcgaggga tcggctgatc cggaggagcc cgacccgaga ggctgagcgc gatgggtctc
      721 ggctagttgg gctcgggccc gtcgacctcc tcgatgagca cctccggggt acgttgctcc
      781 agcccgggct ccacgggatt gatgggatcc ggctcgtttg gcatgtacca cgggcgccag
      841 tgggtgatgg ggacgtcgtc gcgcttgcgc ttgaccagac gaatgaactc gccctcatcg
      901 gtaaggacgg cgtactcgtc cacgttcttc ggctttttct gcgaaaccac gtagtcgccc
      961 tcctcgttga cgtacgagta gaggcgggcc gatgcgggta gcgcgtgcag gcagagtgcg
     1021 agcgcgagga tgccggcaac gacccgctgg ttcatctggt cgatccgctg gctgttttgc
     1081 tggagcggcg tcattgtctg catatcacct caccgcccgg cggaggctcc ggcgtcggcg
     1141 ctgcttggcg aaacggacgt ccggccccgg ctgagcttcc tgccgagatc atcgagtata
     1201 aggtaaccga cgggcaccac cagcagggtg accaccgtgg cgaagacgac gccccaggct
     1261 acggaggtgg ccatggggat gaggatctgc gcctgcacgc tctggttgag cagcaggggt
     1321 gacaggccgg caaacgtagt gagtgacgtc agcaggattg gccggcagcg cgttaccgct
     1381 gcctcgacca ccgcgctgtg gatgtccatg ccctctcgac gccgcgcgtt tacaccgtgc
     1441 accagcacga ggctcgagtt gacgacgacc ccgcaggctg cgatgccacc cgtcaccgac
     1501 atgatcacga tgttggacag cagcccaagc gacttcatga tcatgtgccc ccacacggcc
     1561 ccggccatgg caaaggggat caccgacatg atgatcagcg gctgcgtgta cgagcgcagc
     1621 ggcacggcca gcagagcgta aattaccgcc agcgccatca ggaacatggg gagcaggctg
     1681 gcgatactgt cctcctgctc ccggcgtgtg ccttccaggc tgaagctgac cctggggtgg
     1741 tcgcgcagaa tggtcctcag cggtcctgga ccgaaatcgg ccagcacctc gttcgccgtg
     1801 atcacgtttc gatccacgtc ggcggtcacg ttcaccacgc ggcgaccgtc ggttcgcttg
     1861 atggcggaga agccgcggcc gagatcggcg ctcgcaaccg tggcgaaggg aacctcggag
     1921 ccgttgcggg tgcggatgcg catctcgtca agggacccga gagcgcggcg ctccgcctcg
     1981 gtgtagcgca gcatcacccg gacgtcgtcg cggccgcgct gaatccgctg aatttcttcg
     2041 ccgtagaacg cctgtcggac ctggcgagcc agggcgccca ggttcagccc gagcgcttct
     2101 cccgccggga ggatatcgag cttcagctcc cgttttccgg agcggaaaga gtcggtgata
     2161 tcgatgacgc ccgggtaggc ggccagctcg ctgcgaatct cggccgccac gttcgccaac
     2221 tcttccagat cgggcccggc gagttggatg tcgatctcgg agcctacgct gaaagtggag
     2281 gtcgcgaagg taaactccac ggcatccggg atggggccgg ttgcgtcccg ccatcggttc
     2341 gcgatctcca gggtgctgag gtcgcgttcg tcgctgggcg taagctgcag cgttacctcc
     2401 cccatgtgcc ctcccgtcct ggcggccgga ggcgcgatga cggcgcccag gccggcggag
     2461 ctcacggggt gcgcgcccac ggccgccagg atgaagcgca cgggcggctg atcgggctgt
     2521 tcgctggcga gctctcgctg cagcacctcc gccgtgacgg cgatgttggt gacgatttcc
     2581 tcggtgaccc gggccggcgt tcccagaggc atcgtgagct tcgcgctgat ccggtccgcc
     2641 ggcagcgagg gaaaataagt gaacggcatg tgtccgccgg cgaccaggcc aaccgttatc
     2701 ccgagaatgg ccaccgcgcc ggccgcggtg acgtagcggg cgtccgtggc cgcgctgacc
     2761 aggcgccgga agcggaactg gatgacgtgc tcgaggcggt cggaaaattg cttctgcagg
     2821 gcgatcaccc gagcggcaaa gcgctcgaag cctccttgga tgctccaggc gatcagcacc
     2881 gccgtcgtgc caatgccgat ggcaaaaaag ctgcgcaggt cccaggcgaa ctccatcagc
     2941 acgatggcca ccagcggaac catcatcagg cccacctcgc ccccggcggt ctgcacgcgg
     3001 cggtgaccca gatgcgcggg cagtaccagc tgggattcga tcagcgagaa cgtcaggcaa
     3061 caaagcacga tcacgctgat caccgactgg atctgccctg tgggccccgg cccgaaggtg
     3121 agcggtaaga acgccgtgat cgtggtcagc accccgaaga tgacgggcat ggatacctgt
     3181 cgtgtgccct cgatggccgc gctgatgagc gtttcactcg ggggtgactc acccgcccgg
     3241 gactgcagag agtgcacgcg ctcgccgatc accaccgcgt catccaccag aatgcccagc
     3301 acgagtatga agccgaaggt ggaaatgccg tcgatggaca aaccggccag attggcgacg
     3361 aagattgcgc ccagaaaggc cgcgggtacg cccacgctga cccagaaagc caggcgtgga
     3421 cgtaggaaca aggcgagcat caggagcacc agcatgaatc cctgccgcgc gctgttcatc
     3481 agggtatcca gacgatctcg catgaactcg gaattgtcgt tccagatcgt cagatccacg
     3541 ccctgtggca gacgttcgtc ggcatcatcc aggtaggatt tcacggctct ggtcactgcc
     3601 agaatgtcct ggtcgccca
//
LOCUS       DBNHQR010000154         5370 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_775606, whole genome shotgun sequence.
ACCESSION   DBNHQR010000154 DBNHQR010000000
VERSION     DBNHQR010000154.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5370)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5370)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5370
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_775606"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1476
                     /locus_tag="AC16VR_03430"
     CDS             <1..1476
                     /locus_tag="AC16VR_03430"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl-D-amino-acid deacylase family protein"
                     /protein_id="HIR6010238.1"
                     /translation="GVGFAPCRPGDEKRVIELMEGVEDIPGTALYEGIEWGNWESFPE
                     YIEYLSTKRYTMDIGTQIPHSALRNYVMGERALTHEDATAEDLEAMCRLVQDGLAAGA
                     LGFSTSRTIGHRSVDGEPIPGTFAEKDELLAIARAMREAGRGVFQAVPAGVVGDIAGP
                     ERWTTEQEVELFAEVARASGRKCTFTLIQNGNRPDQWRNCLNIVEQANAEGIELTPQI
                     ATRPIGIVTSLDSYHMFMRRETYLKYADLPLSSRLAELRKPEVRAAILTDKDVPPDQP
                     GVMANVFALMQMAAPAMFPIDIPIDYEPEPSANMGARAAAAGMDIQAFMYDFLTAADG
                     DQFAILLGANFADGTFSVIEEMIPHPNTTIGLSDAGAHVNLIFDAVGPTYQLTHWVRD
                     RSRGRRFPIELMVHKQTYSNARLFGLNDRGSIEVGKRADINVIDLERLGLGKLHVHRD
                     LPAGGSRILQPAHGYVGTFVAGVKTRENDRDTGARPGRVIR"
     gene            complement(1580..2650)
                     /locus_tag="AC16VR_03435"
     CDS             complement(1580..2650)
                     /locus_tag="AC16VR_03435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR6010239.1"
                     /translation="MIRVLALTRYGALGASTRLRTLQYVPLLREDGIDIDVSPLLVDA
                     YLEGLYRRGRGPLLASLAGYTRRLAALTNQRAYDLVWLEKELLPWLPAAFERMLRQPY
                     LVDYDDAIFHRYDQHPRALVRWLLGNKIDLVMRRAQVVVVGNRYLADAARRAGARELE
                     IVPTSVDTQRYRPLAKDSGRNASGDFNVGWIGTPQTAPFLKTLTPVFERLHGSGGFRF
                     TFIGVPAGLDVGVPYEAFPWAESDEVSLLQRLDCGIMPLPDLPFERGKSGYKLIQYMA
                     CGLPVVASPVGENQHIVREGVNGFLASQTDEWIAALQGLRDAADRGRALGMAGREIVE
                     ATYDCRRNAKRIAALLRAAVAQ"
     gene            complement(2647..3537)
                     /locus_tag="AC16VR_03440"
     CDS             complement(2647..3537)
                     /locus_tag="AC16VR_03440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR6010240.1"
                     /translation="MVIVNWNSGDMLATCLDGLAAHCANVIGQVIIVDNASTDSSLDF
                     PMPTSLEVDLIRSGCNLGFAAACNLGASRSGADFLLFANPDLVIGQDILTQPLRRLRG
                     DATIGAVGIGLYDAGSGQRQRTCCRLPTPISMLARSLGIDRLWPRLGYRLSGWDHSSS
                     RKVDHVIGACYLTPRAVFDRLGGFDERFFLYLEDLDFSARIQQAGMDILYLANPCASH
                     VGGGSSRQIKDERLFYMLRSRLQYVHKHMGVWGLIIVGFSTLAIEPWLRILASVLAGS
                     VTGCGEVLRGYGRLFRWLGS"
     gene            3737..5131
                     /locus_tag="AC16VR_03445"
     CDS             3737..5131
                     /locus_tag="AC16VR_03445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6010241.1"
                     /translation="MPAASAGPLRFNEKLGYAFGDTASNFFFQTFNIFLLYYYTDVFG
                     ISAAAVGTLFFVSKLWDAVNDPLMGALADRTNTRHGKYRPYLLWMALPYGLMGYLMFA
                     NPDFDDTGKLVYAWVTYSLMMMLYTAINVPYSALMGVMTPSTTERTLLASYRFVGAFG
                     GGLLISMLVRPMVHYFGAEDEAAGFQVTMALFAVISVVMFWYTFGATRERVRSLPETD
                     VSFGNDVKLLLSNRPWIVLFFAGIFTLSSIAIKNAVTIHYFKYYVGDDSSPILLFFDI
                     TTLFMTIGMLALIAGVACTKWLTARFEKKTLLIALSLGNAVTMVAFFFVPPDQLALML
                     ALNITGSLMAGPTAPLVWAMYADVADYGEWRFERRTTGLVFSASMFAQKFGLTIGAGL
                     AGWMLALFGYVANVTQSDDSLMGIRLMFTLFPAAVSLLNVAVIAFYTLSDAEVESIGR
                     ELEDRKAQAGPAPI"
     gene            5217..>5370
                     /locus_tag="AC16VR_03450"
     CDS             5217..>5370
                     /locus_tag="AC16VR_03450"
                     /inference="COORDINATES: protein motif:HMM:NF025056.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HIR6010242.1"
                     /translation="MPQLFISYSRDDGRAVYPDADWLGGLGYAVHIDRHISPGEQWRE
                     AIANAIA"
BASE COUNT         1062 a         1757 c         1599 g          952 t
ORIGIN      
        1 ggcgtcggct tcgcgccgtg ccgcccgggg gacgaaaagc gcgtgatcga gctgatggag
       61 ggggtcgagg acatccccgg caccgcactg tacgagggca tcgagtgggg caactgggaa
      121 tcgttccccg agtacatcga gtacctttcg accaagcggt acaccatgga tatcggcacc
      181 cagattccgc acagcgctct gcgtaactac gtgatgggcg agcgggcact cacccacgag
      241 gatgccaccg ccgaggacct cgaagccatg tgccggctgg ttcaggacgg tctcgccgcc
      301 ggagccctgg gattctctac ctcgagaacc atcggccacc ggtccgttga cggcgagccc
      361 atccccggga cgttcgccga gaaagacgag ctgctggcca tcgcccgggc catgagggag
      421 gccggacgcg gcgtcttcca ggccgtgccg gccggggtcg tcggcgacat cgcagggccg
      481 gagcgatgga cgacggagca ggaagtcgag cttttcgccg aggtggcccg agccagcggc
      541 cgcaagtgca cgttcaccct gattcagaac ggcaacaggc ccgatcagtg gcgcaactgc
      601 ctgaacatcg tggagcaggc caacgccgag ggcatcgagc tcacgcctca aatcgcgaca
      661 cgccccatcg gcatcgtcac gagcctggac tcctatcaca tgttcatgcg ccgggagacc
      721 tatctgaagt atgcggacct gcccctgtct tcgcggctcg cggagctgag aaagcctgag
      781 gtgagggccg cgatcctgac agacaaggac gtcccgccgg accagccggg cgtgatggcc
      841 aacgtcttcg ccctgatgca gatggccgca ccagccatgt ttcccatcga catccccatc
      901 gactacgagc cggagccgtc ggcaaacatg ggcgcgcgtg cggccgctgc gggcatggac
      961 atccaagcct tcatgtacga ttttctcacc gccgcagacg gcgaccagtt cgccattctg
     1021 ctcggcgcga acttcgccga cggtaccttc agcgtgatcg aggagatgat tccccacccc
     1081 aacacgacca tcggcctttc cgatgcgggt gcgcacgtca atctcatctt cgacgccgtg
     1141 gggcccacct accagctcac acactgggtg cgcgaccgca gccggggcag acggtttccc
     1201 atcgagctga tggtgcacaa gcagacctac agcaatgcca ggctgttcgg cctgaacgac
     1261 cggggcagca tcgaggtcgg caagcgggcc gacatcaacg tcatcgacct ggagcggttg
     1321 ggcctcggca agctgcacgt gcatcgagat ctgccggccg gcggcagccg catcctgcag
     1381 cccgcccacg gctacgtggg cacgttcgtg gctggagtga aaacccggga aaacgatcgc
     1441 gacaccggcg cgcggccggg gcgggtgatc cgatagctac gctagcggtg gtcttccgga
     1501 gctgctggtc ctgccaatag agcaggcgtg cgaccgaaaa aacccgggtt gagacccgca
     1561 aagcggctcg ataccggatt cattgagcaa cggcagcacg caacaaggcc gctatccgct
     1621 ttgcgttgcg tcggcagtcg tatgtcgcct ccacaatttc cctgcctgcc atcccgaggg
     1681 cgcgaccccg atcagccgca tcgcgcagac cctgcaaagc cgcgatccac tcgtcggttt
     1741 gcgaggcaag aaagccgttc acaccttcgc ggacgatatg ctggttttcc cctaccggac
     1801 ttgcaaccac cgggagccca caggccatgt attgaatcag tttgtaaccc gacttgccgc
     1861 gctcgaaggg caggtccgga agcggcatga ttccgcaatc aagccgttgc agcaggctga
     1921 cttcgtcact ctccgcccag gggaacgctt cgtagggcac cccgacgtca agaccggctg
     1981 gcacgccgat aaacgtaaac cggaacccgc ccgatccgtg cagccgttcg aacacggggg
     2041 tcaatgtctt cagaaacggt gccgtctgcg gcgtcccgat ccaacccacg ttgaagtcac
     2101 ccgatgcgtt tcttccagaa tccttcgcca gcggccggta tcgctgcgta tccaccgagg
     2161 tcgggacgat ctcgagttca cgggcaccgg cacggcgcgc ggcgtcggca aggtaacgat
     2221 tgccaaccac cacgacttgc gcgcgacgca tcacgagatc gatcttgttt ccgagcagcc
     2281 accgtaccag cgcccgcggg tgctgatcgt agcggtgaaa aatcgcgtcg tcgtaatcga
     2341 ccaggtacgg ctgccgtaac atccgttcga acgctgcggg gagccagggc agcagttcct
     2401 tctccagcca taccaggtcg taggcacgct ggttggtcaa cgcagcgagc cggcgcgtgt
     2461 agccggcgag ggaagccaga agcggccccc gcccgcggcg atacagcccc tcgagatagg
     2521 cgtcgaccaa gagaggcgaa acatctatgt caatgccgtc ctcacgcaga agcggaacgt
     2581 attgcagcgt tcgcagccgg gtgctggctc caagcgcgcc atatctcgtc aaggcgagaa
     2641 cgcggatcac gagcccagcc aacgaaatag tcgcccgtag ccacgcagga cctcgccaca
     2701 acccgttacc gaaccagcca gcacgctggc cagaatccgc aaccagggct cgatcgcgag
     2761 cgtgctgaac ccaacgatga tcaggcccca cacgcccata tgcttgtgca catactgcaa
     2821 ccgcgacctc aacatgtaga aaagcctctc gtccttgatc tgtcgggatg aaccacctcc
     2881 gacgtggctg gcgcagggat tggctagata gagaatgtcc atcccggcct gctgaattcg
     2941 cgccgaaaag tccagatcct cgaggtagag gaaaaatcgc tcgtcgaatc cgccgaggcg
     3001 atcaaataca gcgcgtggcg tcaggtagca ggccccgatc acgtggtcga ctttgcgcga
     3061 gctggaatga tcccatccac tcagacgata gcccagtcgc ggccagagac gatctatgcc
     3121 aagagaacgc gcgagcatgc tgattggcgt cggtaaccgg cagcacgttc gctggcgctg
     3181 gcctgaacca gcgtcataca aaccaatgcc caccgcgcca atggtggcgt ccccacggag
     3241 ccggcgcagc ggttgggtca atatgtcctg gccgatgacc agatccggat tcgcgaacag
     3301 caggaaatcc gccccagacc gcgacgctcc gagattgcag gcggctgcga atccaaggtt
     3361 gcacccggag cggatcagat cgacctccaa tgaggtcggc atcggaaagt ccagagaaga
     3421 atccgtggat gcattgtcca cgatgatgac ctgaccaatt acgttcgcgc agtgggctgc
     3481 caggccatcg aggcaggtgg cgagcatgtc accggaattc cagttgacga tgaccacgtc
     3541 aacgctccga tctgttgcaa agcaggtatc caacgacagg cgcaacccca caagactcag
     3601 ctcattctag cgacacgcac tcccgggcgc gcaccctctc ccgacctttt cctcagcaat
     3661 cgccatggat aaatgatagc gttgtcatcc tgatttgcga cccgctcgtc gccaggcgac
     3721 cgggagaggg ccagacatgc cagccgccag cgccggaccg cttcgcttca acgaaaagct
     3781 cggctacgcg ttcggcgaca cggcctccaa tttcttcttc cagaccttca acatctttct
     3841 gctctactac tacacggacg tcttcggcat ctcggcggcg gcggtgggaa cgctgttttt
     3901 cgtctcgaag ctctgggatg ccgtgaacga tccactcatg ggtgcgctgg cagaccggac
     3961 caatacccgg cacggaaaat atcgcccgta cctgctctgg atggctctcc cctacggcct
     4021 catgggctat ctgatgttcg cgaacccgga cttcgacgac accggcaagc tggtctacgc
     4081 ctgggtgacc tattccctga tgatgatgtt gtacacggcc atcaacgtcc cctactccgc
     4141 gctcatgggc gtgatgaccc ccagcaccac cgagcgcacg ctgctggcca gctatcggtt
     4201 cgtgggtgcg ttcggtggcg ggctgctgat ttcgatgctc gtccgaccca tggtccacta
     4261 cttcggcgca gaggacgagg ccgcggggtt tcaggtcacc atggcgctct tcgccgtgat
     4321 ctcggtggtg atgttctggt acacgttcgg cgctacccgc gagcgcgtgc gttccctgcc
     4381 cgaaaccgac gtcagcttcg gcaacgacgt gaagctcctg ctcagcaacc gaccctggat
     4441 cgtgctcttc ttcgcaggga tcttcacgct gtccagcata gccatcaaga acgccgtcac
     4501 gattcactac ttcaagtact acgtcggcga cgacagctcc cccatcctgc tcttcttcga
     4561 catcaccacg ctcttcatga ccatcggcat gctggcgctc atcgctgggg tcgcctgcac
     4621 caagtggctg acggcccgtt tcgagaagaa gaccctgctg atcgctctgt ctcttggcaa
     4681 cgccgtgacc atggtcgcct tcttcttcgt cccgccggac cagctcgcgc tgatgcttgc
     4741 gctcaacatc accggttcgc tcatggcggg acccacggcc ccgttggtct gggccatgta
     4801 cgccgacgtg gccgactacg gcgagtggcg ctttgagcga cgcaccaccg ggctcgtttt
     4861 ctccgcttcc atgttcgcgc agaaattcgg cctgaccatc ggcgccgggc tggcaggctg
     4921 gatgctcgcg cttttcggat acgtagccaa cgtcacccag agcgacgatt cgctgatggg
     4981 tatccggctc atgttcaccc tgttccccgc ggcggtgagc ctgctgaacg tggccgtgat
     5041 cgccttctac accttgagcg acgccgaggt ggaatccatc ggccgggagc tcgaggaccg
     5101 gaaggcacag gcggggcctg ctccgatctg agcctcggtg gcagccatcg gcgcctctgg
     5161 cataatcctg agttggggtt tccggggcgg gatccgtggc agaagtatca gaacagatgc
     5221 cgcagctgtt catctcatac tctagagatg acggcagagc cgtatatcca gacgcggatt
     5281 ggcttggcgg tctcggatat gccgttcata tcgacagaca catcagtccc ggagagcaat
     5341 ggcgcgaggc gatcgcgaac gccatcgcgg
//
LOCUS       DBNHQR010000155         2764 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_15096, whole genome shotgun sequence.
ACCESSION   DBNHQR010000155 DBNHQR010000000
VERSION     DBNHQR010000155.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_15096"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="AC16VR_03455"
     CDS             <1..409
                     /locus_tag="AC16VR_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016562483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIR6010243.1"
                     /translation="ITPASGFVGPAGALVIGILAGIFCFAATNYLKRSLKIDDSLDVF
                     PVHGVGGILGTLLTAVFASSALGVFSGQEDIVILDQLGVQAIGVVATMAWTGIATFVI
                     LKVTDALVGNRVSDEEEVEGLDLVLHNERGYDL"
     gene            complement(458..1417)
                     /locus_tag="AC16VR_03460"
     CDS             complement(458..1417)
                     /locus_tag="AC16VR_03460"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR6010244.1"
                     /translation="MSHFELHVAVLMGGPSAEAEVSRVSAAEVAKGLDQAGHRATLLE
                     LDTGAAQALADLTPDVVFPALHGPPGEDGTVQGFLEILNLPYVGSGVHGSAVAMDKSL
                     AKAVFRRAGLPVADDLVVSVGADLAAAEREILDTLGERVVIKPLNQGSAIGVIRVPNG
                     GDLQAALQTALAFGHGVLVEPYVHGREITVGVLDVTGDEPEPFPVIEIITASDEWYDY
                     TNRYTVGKSEHVVPADVPDPVADALREHAVRAHAELGLRDLSRADYILTDANEIVLLE
                     VNTLPGMTPTSLFPDGARATGRDFPTLVDLLVRRAHQRGADGA"
     gene            complement(1414..>2764)
                     /locus_tag="AC16VR_03465"
     CDS             complement(1414..>2764)
                     /locus_tag="AC16VR_03465"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR6010245.1"
                     /translation="MPWDGCQLVRSAFAPDGGLADDVVIAGGAQESVQQPQWLGGDAL
                     IFLSDRNGYSNLYRYDPSGLYCTLEDGADYGVPQWVFGIRQFAAVSDRHVVAARQGTE
                     TELVMVDTEGGFATPLTDAGGAASLGSVVKWQDSVCCLAEFPDRLAAIVRCPLDGSPP
                     TAIHQPGTAPLGAELISRAERVQYPTRDGNRAFAYYYPPRNPEHPVTGAEGELPPLLV
                     MSHGGPTAAASPALNLRIQYYTTRGWAVVDVDYRGSSGYGRAYREALNGRWGVLDVTD
                     CEDAVRHLVDQGLADPDRIAIRGGSAGGFTTLAALTRSSTFKAGASHYGIGDLNALAR
                     DTHKFESRYLHTLIGSEAALDERSPISHADALSAPVIFFQGSDDRVVPPAQSRQMADA
                     LRVRGIPVAYVEFAGEGHGFRRAENIARSLENEHQFFCRVFGLFGDDDVKDLEIENL"
BASE COUNT          466 a          910 c          913 g          475 t
ORIGIN      
        1 catcacgccg gcatcgggct tcgtcggtcc cgccggcgcg ctcgtcatcg gcattctcgc
       61 cgggatattc tgcttcgctg caaccaacta cctcaagcgg tcattgaaga tcgacgactc
      121 gctggacgtg ttccccgtcc acggggtcgg cgggattctg ggaacgctgc tcacggccgt
      181 tttcgccagc agcgccctgg gcgtcttcag cgggcaggag gacatcgtga tcctcgacca
      241 gctcggcgtt caggccatcg gcgtggtcgc caccatggcg tggacgggca tagcgacgtt
      301 cgtgattctc aaggtgaccg acgcgctggt cgggaaccgc gtctccgacg aggaagaggt
      361 ggaagggctc gatctcgtcc ttcacaacga acgtggatat gacctgtaac accctgaaaa
      421 ctatctaagt tttcagggtc atttcccaag ggggcggtca ggctccgtct gcgccccgct
      481 ggtgggccct gcggaccagg aggtcgacca gcgtggggaa atcccttccg gtggcccggg
      541 cgccgtccgg gaaaaggctg gtcggcgtca tcccaggcag cgtgttcacc tccaggagga
      601 cgatctcgtt ggcgtccgtg aggatgtaat cggctctcga caggtccctc aagccaagct
      661 ccgcgtgggc gcgaacggca tgctctctca gagcgtcggc caccgggtcc ggcacgtcgg
      721 ctggaaccac gtgctcgctc tttcccacgg tgtagcggtt ggtgtagtcg taccactcgt
      781 cgctggcggt gatgatctcg atcacgggaa acggctcggg ctcgtccccg gtgacgtcca
      841 gcacgccgac cgtgatctcg cggccatgga cgtagggctc gacgaggacc ccatgcccga
      901 acgccagggc cgtttgcagc gcggcttgca gatcgccgcc gttcggcacg cggatcacgc
      961 cgatggcgga gccctgattc agaggcttta tcactacgcg ctccccgagc gtgtccagaa
     1021 tctcccgctc ggcggccgcc agatcggccc cgacgctcac gacgaggtcg tcggccaccg
     1081 gcaggcccgc acggcgaaac acggccttgg ccaggctttt gtccatggcc acggcgctgc
     1141 cgtgaacgcc gctgccgacg tagggcagat tgagaatttc caggaagccc tgaacggtgc
     1201 catcttcccc cggcgggccg tgaagagcgg gaaacaccac gtccggagtc aggtcggcga
     1261 gggcctgcgc cgctccggta tccagttcaa gaagggttgc ccggtgaccg gcctgatcca
     1321 agccctttgc gacctccgcg gcggataccc tggaaacttc ggcttcggca gatggtccgc
     1381 ccatgagaac ggcaacgtgc agttcgaaat ggctcacagg ttctctatct ccaaatcctt
     1441 tacgtcgtca tcaccaaaga gaccgaatac gcggcagaag aactggtgct cgttctcgag
     1501 cgacctggcg atgttttcgg cccgtcggaa gccgtggcct tcgccggcaa actcgacgta
     1561 ggccaccgga atgccccgca cgcgcagcgc gtcggccatt tgcctggact gtgcgggcgg
     1621 gaccacccgg tcgtcgctgc cctgaaagaa gatgaccgga gccgacagcg catccgcatg
     1681 ggagatgggc gagcgttcat ccagagctgc ctcgctgccg atgagggtgt gcaggtagcg
     1741 tgattcgaac ttgtgcgtat ccctggccag cgcgttaaga tcgccgatcc cgtaatggct
     1801 ggcgccggcc ttgaaagtgc tcgagcgagt cagcgccgcc agcgtggtga accccccagc
     1861 gctgcctccg cgaatggcga tccggtccgg gtctgcgagc ccctggtcga ccaggtggcg
     1921 gaccgcgtct tcgcagtcgg tgacgtccag cacgccccag cgaccgttga gcgcttctcg
     1981 gtaggctcgc ccgtatccgg agctgccgcg gtagtccacg tcgaccaccg cccagccgcg
     2041 ggttgtgtag tactgaattc tcaggttcaa cgctggggac gccgctgccg tggggccacc
     2101 gtggctcatg acgagaagcg gcggaagttc cccctcggcg cccgtaaccg ggtgttcggg
     2161 atttcgaggt ggatagtaat aggcgaaggc cctgttgccg tcgcgggtcg ggtactgaac
     2221 ccgctcggcg cgggagatca gctcggcgcc caacggcgcc gtgcccggct ggtgaatggc
     2281 cgtcgggggc gagccgtcga gcgggcagcg cacgatcgcg gcaagccggt cgggaaactc
     2341 ggcgaggcag cagacgctgt cttgccattt caccaccgag cccaggctcg cagcaccgcc
     2401 ggcgtccgtc aacggcgtgg cgaacccgcc ctcggtgtcg accatgacga gttccgtctc
     2461 ggtgccctgg cgggcggcga cgacatgccg gtcgctcacc gcggcgaact gacgaatgcc
     2521 gaacacccac tggggcacgc cgtagtccgc accgtcctcc agcgtgcagt aaagcccgct
     2581 ggggtcgtat cgatagagat tcgagtagcc gttgcggtcg ctcaagaaga tcagcgcatc
     2641 gccaccaagc cactgaggct gctgcaccga ctcctgggca ccgccggcga tgacgacgtc
     2701 gtcggcgagg ccgccatcgg gagcgaacgc cgatcgcacc agctggcagc cgtcccaggg
     2761 catg
//
LOCUS       DBNHQR010000156         5574 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_205000, whole genome shotgun sequence.
ACCESSION   DBNHQR010000156 DBNHQR010000000
VERSION     DBNHQR010000156.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5574)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5574)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5574
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_205000"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="AC16VR_03470"
     CDS             complement(<1..864)
                     /locus_tag="AC16VR_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010246.1"
                     /translation="MRHLLTSVTRICDLAEGAFDVAPVPKDTWATGDYVVGEVLDASG
                     YHNVELPNGRLMEAMEGDRVVGAFGARAATLEAVGDWQSIQGDEFDALTPAGLFGRLT
                     SVSPFLPRLMGMRYLGHVHANGVRQTMDGAVTPAPDAALTVPIVLITGTSMSSGKTMS
                     GRLIVRLLSRLGFRVVGAKLTGAARYRDVLSYEDAGAAAVFDFVDEGLPSTVGDPADF
                     AARLERLLSRIAGAAPDVVVAEAGASPLEPYNGKTAIDVLGDRVRFNLLCASDPYAVV
                     GVASAFERTPDL"
     gene            complement(875..2047)
                     /locus_tag="AC16VR_03475"
     CDS             complement(875..2047)
                     /locus_tag="AC16VR_03475"
                     /inference="COORDINATES: protein motif:HMM:NF013696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIR6010247.1"
                     /translation="MSRADDIRRHLQAEEPEMLELLTALVTLESPSDVPETQGPVLDE
                     LQRAFDGLGYRTIRVPGRASGGHLYARPGRRRSGGNRRWQLLLGHCDTVWPVGTLEKM
                     PLAADGNHLKGPGIYDMKAGLVEMVFALKALEALGLEPEVEPVIFINSDEEIGSREST
                     RYIRALSQRADRALVMEPSLGPRGRLKTARKGVGRFTVTVRGKAAHAGLDPTAGASAI
                     LELSHVIQKLFALNDPERGASVNVGVIDGGVRANVVAPESSAVVDVRVASQEDAERIE
                     RAILSLEAETPGVSLGVVGRIGRPPLEHTPANRQLWRVATDLACELGIQLEEAMAGGG
                     SDGNTTSLFTATLDGLGAVGDGAHADHEHIQVDQLSQRCALLAMLLLAPPLNSGTG"
     gene            complement(2052..2657)
                     /locus_tag="AC16VR_03480"
     CDS             complement(2052..2657)
                     /locus_tag="AC16VR_03480"
                     /inference="COORDINATES: protein motif:HMM:NF013756.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR6010248.1"
                     /translation="MAGDARATIALDVSESIWERFFCVFPLVVVGTVEPDGTYDLAPK
                     HLAMPMSWKNHFGFVCTPRHRTYQNIERTGEFTVTYARPSQAVLSSLAATPRCEDGSK
                     PITAALPTRPAQTVAGVFLDDGYLFLECRRYRVYDDFGTNSLITGEIVAAGVAEDALR
                     SADEDDQDVIRRAPLLSFLYPGRIAEISASQQLPLPAGFSR"
     gene            2821..3024
                     /locus_tag="AC16VR_03485"
     CDS             2821..3024
                     /locus_tag="AC16VR_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010249.1"
                     /translation="MSAGTNQLAIEYKGRQYSGVYSVSGNLMIARIPGISSRSLETAE
                     ADESQARTLLMEILKEADTLGQL"
     gene            3088..4452
                     /locus_tag="AC16VR_03490"
     CDS             3088..4452
                     /locus_tag="AC16VR_03490"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6010250.1"
                     /translation="MSQKRLSRSTLLYFGLPDYAIYLAAIPVSLYLPFVYSRDLGLDL
                     TDIGLILMLARISDVITDPLIGYLSDRTQTRFGKRKPWLAGGALVMMVSAWQLFAPMG
                     PVTNWHLFIWSMLLWLGWTMINIPYFAWGAELSDDYNERTRITGWRQAFGYLGNVSVL
                     AIPTLSGELFGYGSLPREGLTIVGFMALVLLPVLVVLALWKVPEPEKHRATEANLLVS
                     SRQMFSNGSFMLLFTGFLMLSMGTTWASSMFMLFATFVVDVEGYTQAILLGFYFVQLL
                     SLPLWLQISYRIGKKETWLLGAAIYAIASPGYLMLGSGDVVGLGIVLGLYGIASGNFT
                     AISLSMKADVIDVAALRTGNHVAGSYMAVWSLGQKLFQAIAVGLALPAVAYFGFDPKG
                     ENGPDELLALRLNITVVPMLLYAASVFVIWRYPLTADRLHQLREAFSRRRARREIKQG
                     STGA"
     gene            4475..4939
                     /locus_tag="AC16VR_03495"
     CDS             4475..4939
                     /locus_tag="AC16VR_03495"
                     /inference="COORDINATES: protein motif:HMM:NF017771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HIR6010251.1"
                     /translation="MSSDRIETLERQIFDLTRELHELRKANVGDEVPNYTFATLTGEA
                     SLLDLFGSRDTLLAIHNMGQGCRYCTLWADGLNSLLPHLESAMAVVVLSKDAPETQRL
                     FANSRGWQFRLASHGGGNYMAGRSVLLDLERVCARRAGRIELDAPVQLLAAA"
     gene            complement(4999..5295)
                     /locus_tag="AC16VR_03500"
     CDS             complement(4999..5295)
                     /locus_tag="AC16VR_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010252.1"
                     /translation="MFGDGSVRIIFTPGHTPGHQTLLISLEKTGPVMLSGDLYHFRAN
                     RRLRRPPNFNVDQEQTLQSMDRLEAVLEETGATLWIEHDKALAETLRKAPAFYD"
     gene            complement(5350..>5574)
                     /locus_tag="AC16VR_03505"
     CDS             complement(5350..>5574)
                     /locus_tag="AC16VR_03505"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6010253.1"
                     /translation="GPVAIDGGSVSYERSVVDQLADMDITPEDVDLVAYSHLHWDHAG
                     TANTFIASEVLMQRAEWAAAMGEGGEFVDT"
BASE COUNT          962 a         1860 c         1765 g          987 t
ORIGIN      
        1 cagatcgggc gtgcgctcga aggccgaagc aacaccgacc accgcgtagg ggtccgatgc
       61 gcacagcagg ttgaaacgga cccggtcgcc gagcacgtcg atggccgttt tcccgttgta
      121 gggctccagc ggcgaggcgc ccgcttcggc caccaccacg tccggcgccg cgccagcaat
      181 ccggctgagc aaccgttcca gacgcgcggc aaaatccgcc ggatcaccca ccgtggacgg
      241 caacccttcg tccacgaagt cgaagacggc cgctgcgccc gcatcctcgt agctgagaac
      301 atcgcggtaa cgggccgccc cggtgagctt tgcgccaacg accctgaacc ccaaccggct
      361 caacagcctc acgatgaggc ggccggacat ggtcttcccc gaggacatgg aggtcccggt
      421 gatgagaacg atgggcacgg tcagggccgc gtcaggagcc ggcgtcacgg ccccgtccat
      481 ggtctggcga acgccgttgg cgtgcacgtg ccccaggtag cgcatgccca ttagcctcgg
      541 cagaaaagga gacacggagg tgaggcggcc gaagagcccc gcgggggtca gcgcgtcgaa
      601 ctcatcgccc tgaatgctct gccagtcacc cacggcttcg agggtggccg ctcgagcccc
      661 gaaagcgccg acgacccgat cgccttccat ggcttccatc aggcggccat tgggcagctc
      721 gacgttgtga tatccggagg catcgagtac ttccccgacc acgtagtcgc cggtcgccca
      781 ggtgtctttc gggaccggcg ccacgtcgaa agcgccctcg gcaaggtcgc agatgcgggt
      841 aacggatgtc agcaggtgtc gcacgcgccg atcctcaacc ggttccggag ttcaggggag
      901 gggcaagcag gagcatggcc agcagcgcgc agcgttggga cagctggtct acctgaatgt
      961 gctcgtgatc cgcgtgggcg ccatcgccca ccgcgcccag accgtccagg gtggcggtaa
     1021 acagactggt ggtgtttccg tccgagcccc cgccggccat ggcttcttcc agctgaatgc
     1081 cgagctcgca tgccagatcc gtggccacgc gccacaactg acggttcgcc ggcgtgtgct
     1141 ccagcggtgg ccggccgatg cggccgacca cgccgagaga cacgcccggc gtttccgctt
     1201 cgaggctgag tatcgcccgc tcgatgcgct ccgcatcttc ctggctggcc accctgacat
     1261 ccaccacggc gctgctttcc ggcgccacca cgttggcgcg cacgccaccg tcgatcacgc
     1321 cgacgttgac ggatgccccc cgctcggggt cgttcagggc aaagagcttc tgaatcacgt
     1381 gggagagctc gaggatggcg ctagcgccag ccgttggatc cagacccgca tgagccgctt
     1441 tgccgcgaac cgtaacggtg aatcggccaa cgcccttgcg cgccgtcttc agccggcccc
     1501 ggggccccag ggaaggctcc atgaccagcg cccggtcggc ccgctgcgag agcgcgcgga
     1561 tgtagcgcgt ggactcccgg ctgccgatct cctcatcgga gttgatgaaa atcaccggct
     1621 ccacttcggg ctcgaggccc agcgcctcga gggctttgag cgcgaagacc atctcaacca
     1681 ggcccgcctt catgtcgtag atgcccggac ctttcaggtg gttcccatcc gcagccaggg
     1741 gcattttctc cagggtccct accggccaga ccgtgtcgca gtggccaagg agcagttgcc
     1801 atctccggtt gccgcccgat cggcggcggc ccggccgagc atagagatgg ccgcccgagg
     1861 cgcgccccgg cacccggatc gtccggtagc cgagaccgtc gaaagcccgc tgcagctcat
     1921 cgagcacggg cccctgagtc tcggggacat cggacggtga ctccagggtg accagagcgg
     1981 ttagcagctc cagcatctcc ggctcttccg cctggaggtg gcgacgaatg tcatcggccc
     2041 ggctcatccg gctacctgga gaatcccgcg ggcaggggca gctgctggct ggccgagatc
     2101 tcggcaatcc gccccggata gaggaagctg agcaacggcg cccggcggat cacgtcctga
     2161 tcgtcctcgt cggcgctgcg cagagcatcc tccgccacgc cagcggcaac gatctcgccg
     2221 gttattaggc tgttggtgcc gaagtcgtca tagacgcgat agcgccgaca ttcgagaaag
     2281 aggtaaccgt catcgagaaa gacgccggca accgtctgcg ctggccgcgt cggcaacgcg
     2341 gcggtgatgg gtttgctgcc atcctcacat cgcggggtgg ccgcgaggct cgaaagcacc
     2401 gcctgagaag gccgcgcgta ggtcacggtg aattcgcccg tccgctcgat gttctgatac
     2461 gtccggtggc gcggggtgca gacgaagccg aagtggttct tccagctcat cggcatggcc
     2521 aggtgcttgg gcgccaggtc ataggttccg tccggctcga ccgtgccaac caccaccaga
     2581 ggaaacacgc agaagaaccg ctcccagatg gattcgctga catcgagcgc aatggtcgct
     2641 ctcgcatcgc ccgccatatt ggccctcttc tcaccaggaa agcgtccctt ccactatcgg
     2701 cagtcgcccc aggccggacc atacggggtt tccgagacgc gcccgggccc ttcagtgttc
     2761 gcccaatcgt gttatctttc agtgcaaatc cgacaaacaa cgtgacccgg tggggaggct
     2821 atgagcgcag ggaccaacca gctggctatc gagtacaaag gcaggcagta ctcgggcgta
     2881 tattccgtgt ccggcaacct gatgattgcc cggataccgg gaatcagctc cagaagcctg
     2941 gagacggcgg aagccgacga gtcccaggcc cgcacgctgc tgatggaaat cctcaaggaa
     3001 gcggacaccc tggggcagct ctgagcgccg ccgacctggc gggctgcctg acggtggctg
     3061 acacgcccgt ttaggctcgg gggtctcatg tcgcaaaaac gcctttcgcg ttccaccctt
     3121 ctctacttcg ggctgcccga ctacgccatc tatctggcgg cgatacccgt ctcgctctac
     3181 ctgcccttcg tctactcgcg ggacctgggc ctggacctga cggacatcgg cctgatcctc
     3241 atgctggcgc gcatcagcga cgtgatcacc gacccgctga tcggctatct cagcgatcgc
     3301 acccagaccc ggttcggcaa acgcaagccg tggctcgccg gcggcgccct cgtcatgatg
     3361 gtttccgcct ggcaactgtt cgctcccatg gggcccgtca ccaactggca cctcttcatc
     3421 tggtccatgc tgctgtggct gggctggacc atgatcaaca ttccatactt cgcctggggc
     3481 gccgagctct ccgacgacta caacgaacgt acgcgcataa cgggctggcg ccaggcattc
     3541 ggctacctcg gcaacgtgag cgtgctggcg attcccaccc tctccgggga gcttttcggc
     3601 tacggttccc tgccccggga aggtttgacc atcgtcggct tcatggccct ggtgctgctg
     3661 ccggttctgg tggtcctggc gctgtggaaa gtgccggagc cggagaagca ccgtgccacc
     3721 gaggccaacc tgctcgtgag cagccgacag atgttctcga acggttcttt catgctgctg
     3781 ttcacgggtt ttctgatgct gtccatgggc accacctggg cgtcatccat gttcatgctg
     3841 ttcgccacgt tcgtggtgga cgtggagggg tacacccagg ccatcctgct gggcttctac
     3901 ttcgtgcagc tgctctccct gccgctgtgg ctgcagattt cctaccggat cggaaagaag
     3961 gagacctggc tgctcggcgc cgccatctat gccattgcca gtcccggata cctgatgctc
     4021 ggctctggcg acgtggtcgg cctgggcatc gtcctggggc tgtacggcat cgcaagcggc
     4081 aacttcaccg ccatctccct gtccatgaag gcggatgtca tcgacgtcgc ggccctgcga
     4141 acgggcaatc acgtggcggg ttcctacatg gcggtctggt cgctgggcca aaagctgttc
     4201 caggccatcg ccgtgggcct ggcgttgccc gccgtcgcct acttcggctt cgaccccaag
     4261 ggcgagaacg gcccggacga gcttctcgcg ctgcggctga acatcacggt ggtacccatg
     4321 ctgctctacg ccgcctcggt gttcgtcatc tggcgctatc cgctgaccgc cgaccggctt
     4381 caccagctgc gcgaggcttt ttccaggcgg cgggccaggc gggagatcaa gcagggttcc
     4441 acaggtgcct gattctagaa tcgaggagga aggaatgagc agtgatcgaa tagagacgtt
     4501 ggaacgtcag atcttcgacc tgactcgaga gcttcacgaa ttgcgcaagg cgaacgtggg
     4561 cgacgaggtg cccaactaca cgttcgccac gctgaccggc gaggcgagtc tgctggatct
     4621 tttcgggtcc cgggacacgc tgctcgccat ccacaacatg ggccagggat gccgctattg
     4681 caccctctgg gcggacgggc tgaacagcct tctgccacac ctggagtcgg ccatggcggt
     4741 ggtggtgctg tccaaggacg cgccggaaac ccagcgcctt ttcgccaatt cacggggctg
     4801 gcagttccgt cttgcttccc atggaggcgg aaactacatg gccgggcgat ctgtactgct
     4861 cgatctggaa cgtgtttgcg ctcgccgggc tggacgaatc gagctggacg ccccagttca
     4921 gctactggcg gcggcctgag cagctcgacg acggcggcgc gaacatcctg gactgaaccg
     4981 gcgccacccg gcgcgggctc aatcgtagaa ggcgggcgct ttgcgaaggg tctccgccag
     5041 ggctttatcg tgctcgatcc aaagcgtggc accggtctcc tcgaggactg cctcgagccg
     5101 gtccatggac tggagcgtct gctcctggtc gacgttgaag ttcggcggcc gccgcaggcg
     5161 cctgttggca cgaaagtgat acagatcgcc agaaagcatc accggacccg tcttctccaa
     5221 agagatgagc agcgtctgat ggcccggcgt gtggcccggc gtgaagatga tcctgacgct
     5281 gccgtcgccg aacacgtcgt ggtcgccctc gagaaacgtg acctcggcat cgaccagctt
     5341 gtcgaagagc taagtgtcga cgaactcgcc gccctcaccc atggccgccg cccactcggc
     5401 ccgctgcatc aggacctccg aggcaatgaa ggtgttggcc gtccccgcat ggtcccaatg
     5461 caggtgcgag taggcgacga ggtcgacgtc ctccggggtg atgtccatgt ccgccagctg
     5521 atcgactacg gatcgttcgt agctcaccga tcccccatcg atggcgacgg gccc
//
LOCUS       DBNHQR010000157         3775 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1028712, whole genome shotgun sequence.
ACCESSION   DBNHQR010000157 DBNHQR010000000
VERSION     DBNHQR010000157.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1028712"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="AC16VR_03510"
     CDS             <1..304
                     /locus_tag="AC16VR_03510"
                     /inference="COORDINATES: protein motif:HMM:NF018649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HIR6010254.1"
                     /translation="VQLSRFKADPANRGQLLNTGLWGLSRHPNYFGDALQWWGLSIVA
                     LSGAAWWALLGPVVMTMIFLVLTADILEQGMSRRHPDYEEYVSNTPKFVPRLPGRD"
     gene            456..1403
                     /gene="argC"
                     /locus_tag="AC16VR_03515"
     CDS             456..1403
                     /gene="argC"
                     /locus_tag="AC16VR_03515"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HIR6010255.1"
                     /translation="MIRVFIDGQAGTTGLEIVQRLAARDDVELLEIDPAERKSPAARQ
                     RLFDSADVAVLCLPDDAAREAAVLADGRCRIIDASTAHRIQEGWCYGLPELGSNQRQA
                     LAAAERVANPGCYPQGFILMIRPLIGAGLLDPEQPLAVQALSGYSGGGRTLIEARRAF
                     GSQEAERRNTEPYALSLKHKHVPEMQAYSGTKIAPLFTPSVGQFYQGMLVGVPLFCSQ
                     LRKGADYDGIYHILEEAYALEPFVDVLEPGAETALTDGFLNATACNGTNRLEIMLFGH
                     SEQLLLMARYDNLGKGAAGAAVQNLNLMMGIDETTGLTS"
     gene            1400..2719
                     /locus_tag="AC16VR_03520"
     CDS             1400..2719
                     /locus_tag="AC16VR_03520"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF024301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004069 - L-aspartate:2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase type Ic"
                     /protein_id="HIR6010256.1"
                     /translation="MTTARAIAALSDPELAALKAELSRELELQAGNRLSLDLTRGKPA
                     ADQLDLSAGLDDAIAGNYIASDGTDSRNYGALTGLREARELGAEIMEADPGDIICWGN
                     SSLQLMHICVDLALNQGLWGDGRRWRRDANPKVLTPVPGYDRHFTICEAAGIDMVNVP
                     MTDTGPDMDAVRRATEDASVKGIWCVPKYSNPSGCIYSPECVDALARLPADAAADDFL
                     ILWDNAYAVHDFEFPRAPLASLLDLARKHGTADHVIMFASTSKITYPSAGLAFLAADA
                     KVRKTVEKRLGTASIGPDKVNQLRHARFLGGRVEAHMAGHAALVKPKFDLVESKLREG
                     LAGLEIASWTTPAGGYFVSLDTRPGLAKKVGELAAQAGLTITKPGATFPYGRDPEDRN
                     LRIAPTFASLADLNVAMELLVVCVKLAAVGAEIHQRTDTGGAQKAHE"
     gene            2712..>3775
                     /locus_tag="AC16VR_03525"
     CDS             2712..>3775
                     /locus_tag="AC16VR_03525"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR6010257.1"
                     /translation="MSKWRIEDFPVAPTEGKDRFHDFALPDPLMHAIADLEFEYCTPI
                     QSAVLPWSLADYDVTGQAQTGTGKTAAFLVTIIARLWEYPPAAAPKIGSPRSLVLAPT
                     RELALQIESDAKDLTKYIDLGVECVVGGMDFQKQLDRIKRRPVDVLIATPGRLLDFIS
                     RRHLSLRDVEVLVIDEADRMLDMGFVPDVRRIVYQTPHKRNRQTLFFSATFNDDVMRL
                     ANSWTIDPEHVAITPESVATDTVTQKFWLVAADKKASLLAEFLTRHAPERALIFTNRR
                     DQTHQLHAFLNKRNIRCEALAGDVPQRKRLSTLSRFKDGDIPYVVATDVAGRGIHVEA
                     ISHVINFDLPEDPEDYVHRIG"
BASE COUNT          695 a         1260 c         1188 g          632 t
ORIGIN      
        1 cgttcagctc agccgattca aggcagatcc tgccaaccgg ggccagctgc tcaacacggg
       61 cctgtggggc ctgtcccgcc accccaacta cttcggcgac gcgttgcagt ggtggggtct
      121 gtccatcgtc gcgctgtcgg gcgccgcgtg gtgggcgctg ctcggccctg tggtcatgac
      181 catgatattt ctcgtgctca ccgccgacat tctcgagcaa gggatgtctc gccggcatcc
      241 cgattacgag gagtacgtat ccaacacgcc caagttcgtt ccccggctcc ccggccgcga
      301 ttgacccggg gatccgcggg gggcacagac gggcaaacgg gtgccggaac agccgagggg
      361 ccatcttttc cgtcgcgccg ctgtgacatc gggccgatat tgcctaaaat cgcgcgcccc
      421 taagaaacaa ccccgcgggg aaggacctcg gcaccatgat cagggtattc atcgacggac
      481 aggccggcac cacgggcctc gagatcgtcc agcgcctggc ggcgcgcgac gacgtcgagt
      541 tgctggagat cgatcccgcc gagcgcaaat cacccgcggc ccgacagcgg ctgttcgact
      601 ctgccgacgt tgccgtcctg tgcctgcccg acgacgcggc gcgcgaggct gcagttctgg
      661 cggacggccg ctgccgcatc atcgacgcca gcacggccca ccggattcag gagggatggt
      721 gctacggtct gcctgagcta ggatccaacc aacgccaggc ccttgccgcg gcagaaaggg
      781 tcgccaatcc cggttgctat ccccagggct tcatcctcat gatccgcccc ctcatcggcg
      841 caggcctgct ggaccctgag cagcccctgg ccgtgcaggc gctttccggc tattccgggg
      901 gagggcgcac gctcatcgag gctcgcaggg ccttcggttc ccaagaggcc gagcgccgca
      961 ataccgagcc ctatgctttg tcgctcaagc acaagcacgt ccccgagatg caggcttact
     1021 cgggaacgaa gatcgccccg ctgtttacgc cgtcggtggg ccagttctac caggggatgc
     1081 tggttggggt cccgttgttt tgcagccaac tccgcaaggg cgcagactat gacggcattt
     1141 atcacatcct cgaggaagcc tacgccttgg aacctttcgt cgacgtgctt gagcccggtg
     1201 cggagaccgc cctgacggac ggttttctca acgccaccgc ctgcaacggc accaaccggc
     1261 tcgagatcat gctgttcggg cactcggagc agctgctgct gatggcgcgg tacgacaatc
     1321 tcggcaaagg ggccgccggg gccgcggttc agaacctgaa cctcatgatg gggatcgacg
     1381 agaccacggg cctgacatca tgacgacggc acgagccatc gccgcgcttt ccgaccccga
     1441 gctcgcagcg ctgaaggccg agctgtcccg ggagctggaa ctccaggccg ggaaccgtct
     1501 gtcgctggat ctcacgcgcg ggaagccggc cgcggatcag ctcgacctga gcgcgggcct
     1561 ggacgatgcc atcgcgggca actacatcgc ttcggacgga acggactcgc gcaactacgg
     1621 cgccctcacc ggcctgcgtg aagccaggga actcggcgca gagatcatgg aggccgaccc
     1681 cggggacatc atctgctggg ggaactcgag cctgcagctc atgcacatct gcgtcgatct
     1741 ggctctgaat cagggcctgt ggggcgacgg gaggcgatgg cggcgcgacg caaatcccaa
     1801 ggtgctgacc ccggttcccg gctacgaccg ccacttcacc atctgcgaag cggccggtat
     1861 cgacatggtc aacgtgccca tgacggatac gggtccggac atggacgccg ttcgaagggc
     1921 aaccgaggat gcgtccgtga agggcatctg gtgcgtgcct aagtattcca atccctcggg
     1981 gtgcatctac tcaccggagt gcgtggacgc gctggcccgg ctgccggcgg atgcggcggc
     2041 ggacgatttt ctgatcctgt gggacaatgc ttacgccgtg cacgacttcg aatttccgcg
     2101 ggcgcctctg gcctccctgc tcgatctcgc gcgcaagcat ggcacggccg atcacgtgat
     2161 catgttcgcc tccacatcca agatcaccta ccctagcgcg ggtctggcct tcctggctgc
     2221 cgacgccaag gtgcgaaaga cggtggagaa gcgcctcggc acggcgagca taggcccgga
     2281 caaggtcaat cagctgcgcc acgcgcggtt cctcggcggc cgggtggagg cccacatggc
     2341 cgggcacgcg gcgctggtga agccgaaatt cgacctggtc gaaagcaagt tgcgcgaagg
     2401 tctggcgggc ttggaaatcg caagctggac cacgccggcg ggcggatatt tcgtgtctct
     2461 ggacacacga cccggcctgg cgaaaaaggt gggcgagctg gcagcccagg ccggattgac
     2521 gatcaccaag cccggcgcaa cgtttcccta cggccgggat cccgaggacc gcaacctgcg
     2581 gatcgcgccc acgtttgcaa gcctcgcgga tctcaacgtc gcgatggagt tgctcgtcgt
     2641 ttgcgtcaag ctcgccgccg tcggcgcgga gatccatcaa cggaccgaca caggaggagc
     2701 acaaaaagct catgagtaag tggcgcatcg aggatttccc agtagcccca accgagggca
     2761 aggatcgctt tcacgatttc gccctgccag atcctctgat gcacgccatc gcagacctcg
     2821 agttcgagta ctgcacgccc attcagagcg cggtgctgcc gtggagcctc gccgactacg
     2881 acgtcaccgg ccaggcccag accggaaccg gaaagacggc ggcgttcctg gtcacgatca
     2941 ttgccaggtt gtgggagtac cctcccgccg cggcgcccaa gatcggttcg cctcgttcgc
     3001 tggtgctggc accgacccgg gaactcgctc tgcagatcga aagcgatgcc aaggacctga
     3061 ccaagtacat cgacctcggt gtggagtgcg tggtcggcgg catggacttt cagaagcagc
     3121 tggaccgcat caagcggcgt cccgtcgacg tgctgatcgc cacccccggc cggctgctcg
     3181 acttcatcag ccgccgtcac ctgagcctgc gggacgtgga ggtcctggtc atcgacgagg
     3241 ccgaccgaat gctcgacatg ggcttcgttc ccgacgtccg ccgcatcgtc tatcagacgc
     3301 cccacaaacg gaaccggcag acgctgttct tcagtgcaac cttcaatgac gacgtgatgc
     3361 ggctggccaa ttcctggacc atcgatccag agcacgtcgc catcaccccg gaatccgtgg
     3421 cgacggatac ggtcacccag aagttctggc tggtcgccgc cgacaaaaag gcttcgctgc
     3481 tcgcggagtt cctcaccagg catgcgccgg agcgggctct gatattcacg aaccgccgtg
     3541 atcagaccca ccagctccac gcgttcctca acaagcgcaa tatccgctgc gaggccctgg
     3601 caggcgacgt gccccagcga aaacgccttt cgacgctgtc gcggttcaag gacggcgaca
     3661 tcccctacgt cgtggcaacg gacgtggccg gcaggggcat ccacgtcgag gcgataagcc
     3721 acgtcatcaa cttcgacctc ccggaagatc ccgaggacta cgtgcatcgc atcgg
//
LOCUS       DBNHQR010000158         3633 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1028825, whole genome shotgun sequence.
ACCESSION   DBNHQR010000158 DBNHQR010000000
VERSION     DBNHQR010000158.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3633)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1028825"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="AC16VR_03530"
     CDS             <1..516
                     /locus_tag="AC16VR_03530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR6010258.1"
                     /translation="WIAIGRLDLQTSGLLLFTNDGALADRMMRPATGLDREYAVRVNG
                     KLSESQERRLTDGVMVDGERLQFSDIKYYDGRGTNHWYHVVLMEGRNREIRRLFESVG
                     LSVSRLKRVRFGPVALPSTLPRGRYAELSYEDLKTLYGLLKLPLRARPAKGGRGPRTS
                     FLLPYPDLPIV"
     gene            complement(519..677)
                     /locus_tag="AC16VR_03535"
     CDS             complement(519..677)
                     /locus_tag="AC16VR_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010259.1"
                     /translation="MEDHNVGAKIFEDVFEFEDDFETEAEANVEAFYVALKMALARLE
                     AYATATPE"
     gene            859..1272
                     /locus_tag="AC16VR_03540"
     CDS             859..1272
                     /locus_tag="AC16VR_03540"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR6010260.1"
                     /translation="MRHVGDILAEKSKDIVWIGPLESVYDALKLMNEKDVGAVLVQID
                     EYLVGILSERDYMRKVILDNHAAKQIKVSEVMTRDVVTVTREDTVVHCIALTKKHHVR
                     HLPVVEEGKAIGMISLRDLFLDVIHEKVGEVGDED"
     gene            complement(1312..2250)
                     /locus_tag="AC16VR_03545"
     CDS             complement(1312..2250)
                     /locus_tag="AC16VR_03545"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR6010261.1"
                     /translation="MAEIIARDAKFADPTTTADGKPRASVAPTGLTTLWVNTGTLCNL
                     TCENCYIESSPTNDRLAYISRAEVRAYLDEIAGSDLETEEIGFTGGEPFMNPDIMGML
                     EDSLGAGHRVLVLTNAMRPMMKHARGLLALGSRYGDALTLRVSVDHFEPDRHRQERGP
                     RSWRPMMEGLRWLSESGFAVHIAGRTRWGEDEHTLRAGFAALFAAEEIAVDAHDPASL
                     VLFPEMDTSADVPEITTECWDLLGRRPEDQMCASSRMVVKRRGAETPEVVACTLLPYD
                     ERFAMGPTLASSLRPVALNHPHCAKFCVLGGGSCSA"
     gene            2440..3204
                     /locus_tag="AC16VR_03550"
     CDS             2440..3204
                     /locus_tag="AC16VR_03550"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6010262.1"
                     /translation="MGRVEGKVAIVTGGASNPGLGRTTALTLAREGAIVVVTDVDLEG
                     AEATAAEIRDAGGRALALHQDVTDEGRWAEVIAATVDAHGRLDVLVNNAGIAVLMPLA
                     EMSLADWNRQIEVNLTSVFLGCKHAAPAMREAGGGSIVNLSSVAGLVGLPTCVAYGAA
                     KGGVRIMSKSVAVELAEHDIRCNSVHPGFIWTNMQAQSAGASAPGGGAMPRDRVPLGR
                     MGDPQDIANCVLYLASDESSYVTGAEFTVDAGMTAQ"
     gene            complement(3276..>3633)
                     /locus_tag="AC16VR_03555"
     CDS             complement(3276..>3633)
                     /locus_tag="AC16VR_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017517981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6010263.1"
                     /translation="RTLLPLLNAAPTASVVLVSSTVGRKGRAYWGAYAVSKHALEGLM
                     EVLADEHEHAGIIRVNSLNPGATRTAMRAAAYPAENPASVPTPEARMDVFVYLMADAS
                     NGISGEALDAREWRGP"
BASE COUNT          598 a         1160 c         1222 g          653 t
ORIGIN      
        1 tggatcgcca tcggccggct cgatctgcaa acgagcgggc tcctcctctt caccaacgac
       61 ggggcccttg ccgatcgcat gatgcgcccg gcgacgggtc tcgaccggga atacgcggtc
      121 cgcgtcaacg gcaagctcag cgaaagccag gagcgtcggc tcaccgatgg ggtgatggtc
      181 gacggcgagc ggctgcaatt ctcggacatc aagtactacg acgggcgtgg caccaatcac
      241 tggtatcacg tggttctgat ggagggccgg aatcgtgaga tcaggcgact gttcgagagt
      301 gtcggcctgt cggtcagccg gctcaagcgg gtccggttcg gtcccgtggc gcttccttct
      361 accctccctc gcggacgcta tgccgagttg agttacgagg atctgaaaac cctgtacggc
      421 ctgctcaagc tgccgctgcg ggctcgcccc gccaagggcg ggcgtggtcc gcgcacctct
      481 tttctgctgc cgtaccccga cctgccgatc gtctgaggct attccggcgt ggcggtcgcg
      541 tacgcttcga gccgggcgag tgccatcttg agggcgacgt agaacgcttc cacgtttgcc
      601 tctgcttcgg tttcgaaatc gtcctcaaac tcaaagacgt cttcgaagat ctttgcgccg
      661 acgttgtgat cttccatgat ctgactatcc cgttgttgtt ggttggtgag ccagatctta
      721 ttcaagtcgt ccccccgggc tgtgacgcgg ttcccagttt cgcgccatgg caggtacccg
      781 ctgacccggt catcgggcgg ttgcttacag cgtcggggcg atctactgtt aggcggcggc
      841 aatctggggg gatactgaat gcggcacgta ggggacatac tggccgagaa gtccaaagac
      901 atcgtctgga tcggcccgct cgaatccgtg tacgacgcgc tcaagctcat gaacgaaaag
      961 gacgtgggcg ctgtgctcgt gcagatcgat gagtatctcg tcgggatcct gtcagagcgg
     1021 gactacatgc ggaaagtgat tctggacaat catgcggcca agcagatcaa agtctccgag
     1081 gtgatgaccc gggacgtggt cacggttacc cgggaagaca ccgtcgtcca ttgcatcgcg
     1141 ctgacgaaaa agcaccacgt ccgtcatctt cccgtggtgg aggaaggaaa ggccatcggc
     1201 atgatctcgc tgcgcgacct ctttctagac gtcatccacg agaaggtggg tgaagtcggc
     1261 gacgaagact gacagcggcg cgtccagcgg ccgtccatcg tgtgaggagc gtcaggctga
     1321 gcagctgccg ccgccgagga cgcagaactt ggcgcagtgg ggatggttca gcgcgaccgg
     1381 ccgcaggctg gaagccagcg tagggcccat ggcgaagcgc tcgtcgtagg gcagcagcgt
     1441 gcacgcgacc acctcggggg tttccgcccc cctgcgcttg accaccattc gagagcttgc
     1501 gcacatctga tcctccggcc gccgtcccag cagatcccag cattcggtgg tgatctcggg
     1561 cacatcggcg ctggtgtcca tctccgggaa cagcaccagg ctcgcgggat catgcgcgtc
     1621 gacggcgatc tcctcggcgg caaacagggc ggcgaagccg gcccgaaggg tgtgctcgtc
     1681 ctctccccag cgggtgcgcc cggcgatgtg aacggcgaag ccggactcgc tcagccagcg
     1741 taacccctcc atcatcggcc gccaggaacg cgggcctctc tcctgtcggt ggcggtcagg
     1801 ctcgaagtgg tcgactgaaa cccgcagcgt cagagcgtcc ccgtagcggg agcccagggc
     1861 cagcagcccg cgcgcgtgct tcatcatcgg ccgcatggcg ttggtgagca ccagaacgcg
     1921 atggcccgcc cccaggctgt cctcgagcat gcccatgatg tcggggttca tgaatggctc
     1981 gccgccggta aagccgatct cctcggtttc gaggtcggag ccggcgatct cgtcgagata
     2041 tgctcgaacc tcggcgcgag agatgtaggc caggcggtcg ttggtcgggc tggactcgat
     2101 gtagcagttc tcgcaggtca ggttgcacag ggtgccggta ttgacccaca gggtcgtcag
     2161 tccggttgga gccacggatg ccctgggctt gccgtctgcc gtggtggtcg ggtcggcgaa
     2221 cttcgcgtcc cgcgcgatga tctcggccat ctctgattgc ccttgcgccg gcgtttgcgg
     2281 cgctcgtcct gctcgatcgg tctctcatgc tgacaggccg ccttctcccc cgcaacgggc
     2341 gtacacgggt tcgtccggaa agacgagctt tgtccgacac aagccggttt gccatactcg
     2401 cgacctcttt tctgcagtcg acacaccggg agcggcgata tgggacgagt tgagggcaaa
     2461 gtggccatcg tgacgggcgg ggcgtccaat cccggcctcg gccgcacgac ggcgctgacg
     2521 ctggcgcgcg agggcgcgat cgtcgtggta accgacgtgg atctggaagg tgccgaggcc
     2581 accgcggcgg agataaggga tgcgggagga agggcgctgg cgctgcacca ggacgtcacc
     2641 gacgagggcc gctgggccga ggtcatcgcc gctacggtgg atgctcacgg caggctggac
     2701 gtcctggtca acaacgccgg gatcgcggtg ctcatgcccc ttgccgagat gtctctggcg
     2761 gactggaacc ggcagatcga ggtcaacctg accagcgtct tccttggctg caagcacgcg
     2821 gcaccggcaa tgcgggaggc aggcggcggc tccatcgtca acctctcgtc cgtggccggg
     2881 cttgtgggcc tgcccacctg cgtcgcctac ggcgcggcca agggcggggt ccggatcatg
     2941 tccaagagcg tcgccgtgga gctcgccgag cacgacattc gctgcaactc ggtccacccc
     3001 ggattcatct ggaccaacat gcaggctcag tccgcgggtg cgtcagcccc cggcggtggc
     3061 gcgatgcccc gggaccgggt gccgctggga cgcatgggtg atccccagga catcgccaac
     3121 tgcgttctct acctcgcctc cgacgagtcg agctacgtca ccggcgccga gttcaccgtg
     3181 gatgcgggta tgacggcgca gtagctggcc ggctccgttc aggtcctccg gtaccgcgtt
     3241 ggtttgagat cggcccgccg cagcgagcag ggctgtcacg gaccgcgcca ctcacgcgca
     3301 tccagcgcct ccccgctgat gccgttcgag gcgtccgcca tcaggtagac gaacacgtcc
     3361 atccgcgcct ctggcgtcgg cacgctggcg gggttctcgg caggataggc ggcagcccgc
     3421 atggccgtgc gcgtggcgcc cgggttcagg ctgttcacgc ggatgatgcc ggcgtgctcg
     3481 tgctcgtcgg ccagaacctc catcaggccc tccagcgcgt gcttggaaac ggcgtaggct
     3541 ccccagtagg cccggccctt gcgccccacc gtggaggaga ccagaaccac cgaggcggta
     3601 ggcgccgcgt tcaggagcgg cagcaacgtg cgg
//
LOCUS       DBNHQR010000159        13319 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1661857, whole genome shotgun sequence.
ACCESSION   DBNHQR010000159 DBNHQR010000000
VERSION     DBNHQR010000159.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 13319)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13319)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13319
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1661857"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="AC16VR_03560"
     CDS             complement(<1..628)
                     /locus_tag="AC16VR_03560"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6010264.1"
                     /translation="MRIAVVGAGVSGIAAAYRLQQEADVVLFEGQGRLGGHTDTHAVL
                     VGGATHAVDSGFIVFNPDNYPRFSSWLDELGVESQPSDMSFGVSNQATGVEYGSRGLG
                     ALFCQRRNLVSPRFLRMLADLRRFYRDAVDYPLDESLTLGELLERHGYGAGFVEDHIG
                     PMCGALWSAAPDAALAIPARHVIAFMTHHRMLQLDGRPQWRVIKGGSST"
     gene            complement(625..1524)
                     /locus_tag="AC16VR_03565"
     CDS             complement(625..1524)
                     /locus_tag="AC16VR_03565"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007152109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006636 - unsaturated fatty acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HIR6010265.1"
                     /translation="MLATLSRQLDSERAMVRRGRGLDWLRIWPFIGLHLACLAVFWVG
                     VSPVAVVVAVACYLIRMFAITAFYHRYFAHKAFRTSRACQFVFAVLGAASTQRGPLWW
                     AAHHRRHHMHTDTADDPHRPQDGFLWSHMGWFLSGEHFQTDMARVRDWSVFPELVWLN
                     RFDTVVPILLGTAMFALGWGLERLAPGLGTSGWQMLIWGYFISTVALIHATLLVNSLA
                     HRSGRRCFDTRDESRNNWIVAVLTLGEGWHNNHHRFAGAARQGFYWWQLDVSFYLLKL
                     MSWLGLIWDLKPVPGSVLREGRS"
     gene            complement(1625..3232)
                     /locus_tag="AC16VR_03570"
     CDS             complement(1625..3232)
                     /locus_tag="AC16VR_03570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR6010266.1"
                     /translation="MAKWQQPRSFWTWGYRSDEPSEEERHQAAARVSERLGRVVEPPP
                     IPRIGEIALRKPRLSVPAGLRGWVTTDHEERVVHTHGGHPLELLSAVRGEFDAPPDAV
                     AHPRNETELEATLAWCDEHGHAAIPFGGATSVVWGVRVPESADAAVTIDMDHFHQVLE
                     IDEVSRAARIQAGVLGPDLENTLRPLGLTLRHFPQSFPWSTVGGWVATRSGGHYATNH
                     THIDDFVESVRMLTPAGWWESRRLPGSGAGPSPDRMVMGSEGILGVISECWLRLQKRP
                     VYRATAGVVFDSWEAGAEAARQIVQAKLWPANLRLLDPAEAAQSAGLGGGRSLLIVGF
                     ESADLPQGDLMRDALAIARGAGGLVDDDAVRVDDGSGKATGREGSVGAWRSAFIGVNA
                     GLTSGLGLLADTFETAITWDRWPEFDASVRERVGRVLEEVLGPGARLSCRFTHVYPDG
                     PAPYYSFSGVAGLGAEHEQWRVIKDEATSAVVDAGGTVTHHHAVGRMHRAGWEDQHPP
                     LYAQALRAMKRTLDPNGILNPGVLIDP"
     gene            complement(3225..4499)
                     /locus_tag="AC16VR_03575"
     CDS             complement(3225..4499)
                     /locus_tag="AC16VR_03575"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIR6010267.1"
                     /translation="MAGERTLPVRLALAVALLVSSASAEPAAAGESTVIVLSWDGMRH
                     DYPDRGDFPGLARVISEGARAERLVTVFPSSTFPGHVSLATGTYPDRHGIVGNHFVDR
                     QRGVYRMDSDADWLLAEPIWIAAERQGVMAATYFWVGSESDWRGRGTRYRESPFDGSR
                     PEALKVDRILEWLQLPEGQRPRLIMSYWAGADRIGHRFGPDSERVTTQIAAQDLQLQR
                     LLQGLDLLDRWASTTLLIVSDHGMTASYRYTDLRGMLAAAGIGAYVTGSTVVHVYLDD
                     PDDHARAGELLGKIEGARAYPGDALPGWMRLSHAARTGDWLVVATPPLTFSRPAGWEG
                     AMVATLSMVGWTFGSHGYPPDHPEMPGMFLALGRGVPAGALLRAVHQVDLAATIAALL
                     GIEPPLESEGTPVPGLEIPVAARQAAKEDDDG"
     gene            complement(4489..5223)
                     /locus_tag="AC16VR_03580"
     CDS             complement(4489..5223)
                     /locus_tag="AC16VR_03580"
                     /inference="COORDINATES: protein motif:HMM:NF013934.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HIR6010268.1"
                     /translation="MLEDVTRFGSKADARQWAWDALDERRLARFPFPPHGRIPNFAGA
                     AAAAMRIFAQAPWSEAGAIKVNPDSPQLPVRFLALQRGIRVYVPTPKLAAGFILLDPA
                     RIPPERFREAAALKTMLRWARPVALEDLPQLDAIVTGCAAATSSGKRAGKGAGYSDLE
                     FAILRELGHDPVPVATTVHDVQLVEDFPIAHNDLPLSLICTPTRTIRVQAPPAAPAGI
                     QWGELSAEQLAAMPVLESLRRLKHGG"
     gene            5229..6527
                     /locus_tag="AC16VR_03585"
     CDS             5229..6527
                     /locus_tag="AC16VR_03585"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6010269.1"
                     /translation="MVPANTVTAYYTAAQRDGMTNFLLQRYPAFGFPRYRKYWLASLA
                     SVGATQLITLAQGWLVFELSGSALQLGILGAAVAAPNIAMTLIGGVFADRFDKRLILV
                     WTSGAIATLLALLAWLDYSGTVRVWHVLAVAAAVSLITGLDWPARVSIFPQLIDRPAF
                     MSAVALNSIIWQATRMAMPALGGIIIAASDTWVLFALGSGGFLTMMIVISGLHVTTSD
                     ARHGSPLEQIAEGVRFIVNEELFKWLMLLTFIGMFFAGSYVQIMPVFADVLESGEAGY
                     GYLLSAGGVGAVAGTLLVGGVQEHARLGFVMLGGATLSAALTAVFALVAVGGWFIAAL
                     LATFFASVFTSVFMISSMAVLQLVVPDDLRGRVMGIHAIGYSLIPLGGLFMGALAEAA
                     GPALAVLIGCCVFGFAIAGVAMTSTTIRGLDGRLIGHKQA"
     gene            6524..6742
                     /locus_tag="AC16VR_03590"
     CDS             6524..6742
                     /locus_tag="AC16VR_03590"
                     /inference="COORDINATES: protein motif:HMM:NF022596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HIR6010270.1"
                     /translation="MSEVGKWLVLAGALTVIVGLLLQTGWLGWFGRLPGDIRVERPGL
                     SFYFPITSMLIVSAILSVAAWILRRFLG"
     gene            complement(6746..7378)
                     /locus_tag="AC16VR_03595"
     CDS             complement(6746..7378)
                     /locus_tag="AC16VR_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010271.1"
                     /translation="MIYLVVNIVGYLLVALVAGVGVGWLLHGSLAGKASGERRGLGSV
                     GVDDLELAARADAQDEQLRKLAATIRQKEREVEALSRELERRDRRFNAADKLAKISRR
                     LQQEAEQLKEQVRVLGEQLEAAREEAQGSHGASAAMEEVLRSEILELEGRLRESSQEQ
                     DRLAKTLEQENRKVEELERQRDLQNSSLQVLHKQLELARKSREGAAEAGG"
     gene            complement(7375..8043)
                     /locus_tag="AC16VR_03600"
     CDS             complement(7375..8043)
                     /locus_tag="AC16VR_03600"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR6010272.1"
                     /translation="MDLRFSQFEILTAAGAFFLCLAFICVFNEASDIQDDVGIRAQGE
                     AREHGLYWAGVEALGQRVRLTGAAPSDAQRLHAAAAVAALPGVTSVQNEIEVLGADGA
                     CQRQMDSALAELGVTFKTGRAEIGDSSYPLIAELARTMGACATPIEIAVHTDSLGDAA
                     VNLKLSQRRADAVRKALVQRGVRPDRLVAAGYGETQPVASNARESGRRENRRVEFRVV
                     GGTA"
     gene            complement(8210..10861)
                     /gene="aceE"
                     /locus_tag="AC16VR_03605"
     CDS             complement(8210..10861)
                     /gene="aceE"
                     /locus_tag="AC16VR_03605"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014603513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HIR6010273.1"
                     /translation="MAERNPDDWLSEQQEWCDAIDDVLAFRGEDEVKELLRRLQAHVS
                     HRGMILTDAALNTPYKNTIDVRDQPAYPGNIELEMKIGDIICWNAVAMVLQAYDSGSG
                     VGGHIATYLSAATMMEVGFNHVFRGASESYGGDQVHFQAHTSPGVYARAFLEGRLSEQ
                     ALTNFRRELAPGGGLPSYPHPRRLPWFWQMPTASMGLSTPSAIYQARFAKYLESRGLK
                     ANNGGKIWTFIGDGESDEPEVLGTINIASRERLDNLVLVINCNLQRLDGPVRGNGKII
                     QELERAFRGADWHVIKVIWGGGWDAILARDEHGILQERMEQAVDGDYQMYTVSSGDVV
                     REHWVETSPELEELMKTLSDEEIRSIKRGGQDHQKIYAAFARAANVRGKPCVVLLKTV
                     KGDGLGPGTEGSNTVHQKKQLNEQERQELAKRLGIPLTREEISRAAFYRPAEDSAELV
                     YLRDRRHSLGGYLPAREVRCAALEPPSVELFAAMLKGSDRPVTTTAAVVRMLSRLLRD
                     PALGRYIVPIVPDEARTFGMDGLFPQAGIYSPAGQRYQPVDAGTIAPYREAEDGQILQ
                     EGICETGAMASFLAAGTAYANYGLPMIPFYIFYSIFGFQRVGDMIWAAGDMMCRGFLL
                     GGTSGRTTLNGEGVQHQDGHSHLVANTIPNLKSYDPAFAFEIALLVREGIRRMYQLQE
                     DVFYYLTLTNQPYPMPAMPEGVEDGVLKGMYCFSRAPGAVVNLFGSGALMTEVLRAES
                     LLAEHGIACNVWSVTSYNELARQALASERARLLGVGDGDERAYAEALLEGEEGIFVAA
                     SDYQKSLPMSISRWVPGPYTVLGTDGFGLSEDRRVLRGYFEVSAEWIAFAALSSLAQN
                     NRLERDVALGFAAKAGLALDKANPAEG"
     gene            complement(10999..11793)
                     /locus_tag="AC16VR_03610"
     CDS             complement(10999..11793)
                     /locus_tag="AC16VR_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017849031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR6010274.1"
                     /translation="MHLESDVAPEEAAHSLRLLTFNIQVGIRTTKYRHYVTKGWKHVL
                     PHEARAINIRHIADLVRDYDLVALQEIDGGSLRSGFINQVEYIADRARFPYWYTQCNR
                     DLGPFAQHGNGVLSRIAPVHMEDHKLPGAIPGRGAILMRFKLGESTVSLVVLHLSLGG
                     RSRQVQLEYVRELIDAEEHVVVMGDMNSHLAQLLFNSPLSETDLVPADQVHPTYPAWQ
                     PAFALDHVLVSPGLSIDDYEVLDCRLSDHRPIAVTLRLGEPVTIQQ"
     gene            complement(11935..12627)
                     /locus_tag="AC16VR_03615"
     CDS             complement(11935..12627)
                     /locus_tag="AC16VR_03615"
                     /inference="COORDINATES: protein motif:HMM:NF024853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HIR6010275.1"
                     /translation="MRDVLAFLLVVALGATGPAFAQEWQEGVHYQRLPVSVATADAAR
                     VEVVEVFSYACIHCKTFDPALEAWRENQPEDVGFRRVPAIFSAEWETLAKAFYTAQIL
                     DLGGAVHDSIFRAVHEEGRDLREPGAIAALFQQVAGVNPQQFAQVFNSFGVRSRIEQA
                     KAHARAYRVSGVPALVVDGTYRVDGRMAGSNAAMLEVVNHLVAKQRAARGIAAPEPAS
                     VEEEDAATSGAS"
     gene            complement(12759..>13319)
                     /locus_tag="AC16VR_03620"
     CDS             complement(12759..>13319)
                     /locus_tag="AC16VR_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018403943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6010276.1"
                     /translation="AAGDPEAGAAQAVVCAACHGQDGASGVDPSYPNLAGQNEKYLYN
                     QLVMIQKDDRKILLMTGQLIGKSDQDLQDLAAYYASLPAKLGQAEGDDDSIALAEQIY
                     RGGIAKKGVAACAACHAPSGEGNASAGFPAVGGQAGSYTIAQLTAYREGRRQTDEVYG
                     GMMRGVAGGLTDGEIAALADYLQGLH"
BASE COUNT         2299 a         4494 c         4302 g         2224 t
ORIGIN      
        1 aggtggacga cccgcccttg atcacccgcc actgcgggcg accgtccagc tgcagcatgc
       61 ggtgatgcgt catgaaggcg atcacgtggc gggccggtat cgccagagcc gcgtccggcg
      121 cagcggacca gagcgcaccg cacatgggcc cgatgtgatc ctcgacgaac ccggcgccgt
      181 agccgtgacg ttcgagcagc tcgccgaggg tgagcgactc gtccagggga taatcgacgg
      241 cgtcacggta gaacctgcga agatcggcca gcatgcgcag gaaccggggt gagaccaggt
      301 tgcgccgctg acagaacaaa gcgcccagtc cgcggctgcc gtactcgacg ccggtagcct
      361 ggttcgagac gccgaaggac atgtccgacg gctgagattc aacccccagc tcgtccagcc
      421 acgacgagaa acgcgggtaa ttgtccgggt tgaacacgat gaatccggag tccacggcgt
      481 gggtggcacc gccgacgagc acggcgtggg tgtcggtgtg accgcccagc cgcccctgcc
      541 cctcgaagag gacgacgtcg gcctcctgct gcagccgata ggcggcggcg ataccggata
      601 cgcccgctcc caccaccgcg attctcacga gcgcccctcc cggagcaccg agccagggac
      661 cggcttgaga tcccagatca agccgagcca ggacatgagc ttcagcaggt agaaagagac
      721 gtcgagctgc caccagtaga atccctgtcg ggcagcgccg gcaaagcgat gatgattgtt
      781 gtgccagccc tcgccgaggg tcagaacggc gacgatccag ttgttgcggc tttcgtcccg
      841 cgtgtcgaag cacctgcggc ccgagcggtg cgccagggag ttcaccagca gggtcgcgtg
      901 gatcaacgcg acggtagata tgaaataacc ccagatcagc atctgccagc cgctggtgcc
      961 gaggccgggt gccagacgtt cgagccccca tcccagagcg aacatggccg tgccgagcag
     1021 gatgggcacc accgtgtcga accggttgag ccagaccagc tccgggaaga cgctccagtc
     1081 gcgaacccgg gccatgtcgg tctgaaaatg ctcgccggag agaaaccagc ccatgtggga
     1141 ccacaggaag ccgtcctgtg ggcggtgcgg atcgtccgcg gtgtccgtgt gcatgtgatg
     1201 tcggcggtgg tgcgccgccc accacagcgg tccccgctgg gtggatgccg cgccgagcac
     1261 ggcaaacacg aactggcagg cgcgcgaggt gcgaaacgcc ttgtgcgcaa agtaacgatg
     1321 gtagaaggcc gtgatcgcaa acatccggat caggtagcag gccacggcga ccacgaccgc
     1381 caccgggctc accccgaccc agaacacggc caggcaggcc aggtgaaggc cgataaaggg
     1441 ccagatgcgc agccagtcca agccgcggcc gcggcgcacc atggcccgtt cggaatccaa
     1501 ttgcctggaa agcgtggcca gcatcgcccc caccctgaac gaagaggacg gccactatgc
     1561 gcggccgcgc gtgaaagcca cgtcaacagg ggagcagacg agcgagtcgc aggcgtgggc
     1621 cgtcctaagg gtcgatgagc acgccgggat tgaggatccc gttgggatcc agggtgcgct
     1681 tcatcgctct gagcgcctgg gcatagagag gtggatgctg atcctcccac cccgcacggt
     1741 gcatgcgccc tacggcgtgg tgatgcgtca ccgttccgcc ggcgtccacc accgcagacg
     1801 tggcctcgtc cttgatgacc cgccattgct cgtgctctgc cccgagcccg gcaacgccgg
     1861 aaaagctgta atagggagcg gggccgtcgg ggtagacgtg ggtgaagcgg caactgagcc
     1921 tcgctcccgg cccgagcacc tcctcgagca cccgacccac cctttcgcga accgacgcgt
     1981 cgaactcggg ccagcggtcc caggtgatgg ccgtctcgaa ggtgtccgcg agcagaccga
     2041 gcccgctggt cagcccggcg ttgacgccga tgaaggccga tcgccaggcc cccacggatc
     2101 cttccctgcc ggtggctttc cccgacccgt cgtccacgcg gaccgcatcg tcgtccacca
     2161 ggccgcccgc tccccgtgcg atggccaacg cgtcgcgcat cagatcgccc tgaggcaaat
     2221 ccgcggactc gaaaccgacg atgagcaggg accggccgcc gccgaggccc gcgctctggg
     2281 cagcctcagc cggatccagc aggcgcaggt tggcgggcca gagcttggcc tgcacgatct
     2341 gcctggcggc ttccgcaccc gcctcccagc tgtcgaacac cacgcccgcc gtggcccgat
     2401 aaacgggccg tttctgcagc cgcagccagc attccgagat gacgcctaga atgccttccg
     2461 agcccatgac catgcgatcg gggctgggtc cggcgccgct gccggggaga cgccgagatt
     2521 cccaccatcc ggccggggtg agcatgcgaa cgctctcgac gaagtcgtcg atgtgggtat
     2581 ggttggtcgc gtagtgcccg ccggaacggg tcgcgaccca gccaccaacg gtggaccagg
     2641 gaaagctctg ggggaaatgc ctgagcgtca ggcccagagg acggagggtg ttctccagat
     2701 cgggccccag gacgcccgcc tggatgcggg ccgcccggga cacttcgtct atctccagca
     2761 cctgatggaa gtgatccatg tcgatggtca ccgcggcatc cgcgctctcg ggcacccgca
     2821 cgccccagac cactgaggtg gcgcccccaa agggaatcgc ggcgtggccg tgctcgtcgc
     2881 accaggcgag cgtcgcctcg agttctgttt cgttgcgcgg gtgggctacc gcatccggcg
     2941 gcgcgtcgaa ctctcctcgc acggcagaga gcagctcgag cgggtgaccg ccgtgggtgt
     3001 gcaccacacg ctcctcatga tcggtggtca cccaaccccg caggccggca ggaacggaca
     3061 gccgcggctt gcgcagcgca atttccccga tccgcgggat ggggggcggc tcgaccaccc
     3121 ggccaagccg ttcggatacc cgagccgcgg cctgatggcg ctcctcctcc gagggctcat
     3181 cagaccgata gccccacgtc cagaagcttc ggggttgctg ccacttagcc atcgtcatcc
     3241 tccttggccg cctgccgggc ggcaacggga atctccaggc ccggcaccgg cgtgccctcg
     3301 gattcgagcg ggggctcgat gcccaggagc gcggcgatgg tggcagccag atccacctga
     3361 tgcaccgccc gtagaagtgc ccctgccgga acgccacggc cgagcgccag gaacatgccc
     3421 ggcatctcgg gatggtccgg cgggtagccg tggctgccga aagtccatcc caccatgctc
     3481 agggtcgcca ccatggctcc ctcccaacca gccggccggc tgaaggtcag gggaggagta
     3541 gcgaccacca gccagtcgcc cgttctggcc gcgtgggaca ggcgcatcca acccggcagc
     3601 gcatccccag ggtaggcccg cgcgccctct atcttgccga gcagttcccc tgcccgggca
     3661 tggtcatccg gatcgtcgag gtagacgtgc accaccgtgc tgcctgtgac gtaggcgccg
     3721 atgccggcgg cggccagcat gccgcgcaga tcggtgtaac ggtagctcgc ggtcatgccg
     3781 tggtcgctaa cgatcaggag cgtggtagac gcccagcgat ccagcaggtc gaggccctgg
     3841 agcagccgct gcagctgcag atcctgagcg gctatctggg tggtgacgcg ctcgctgtcg
     3901 ggaccgaaac ggtggccgat ccgatcggcg ccagcccagt agctcatgat gaggcgcggc
     3961 cgctgcccct ccggaagctg cagccactcg agaatccgat ccaccttcaa agcttcgggt
     4021 cgggaaccgt cgaaggggct ctcgcggtaa cgggtgcccc ggccgcgcca gtcgctctcc
     4081 gagcccaccc agaagtaggt cgccgccatg accccctggc gctcagccgc gatccagatg
     4141 ggctcagcca gcagccagtc tgcatcggag tccatcctgt agacgccgcg ctgccggtcg
     4201 acgaagtgat tgccgacgat gccgtgccgg tccgggtacg tgcccgtggc taaagagacg
     4261 tgtcccggaa aagtagagga aggaaacacg gtaacgagcc gctcggcccg ggcaccttcg
     4321 ctgatcaccc gggccaggcc gggaaagtcg cccctgtccg gatagtcgtg gcgcatgccg
     4381 tcccaggaaa gcacgatcac cgtgctctcc ccggcggcgg cgggctcggc cgaagccgag
     4441 ctgacaagaa gcgccacggc cagtgccagg cgcacgggaa gggtgcgctc acccgccatg
     4501 cttgagcctg cgcaaggatt ccagaaccgg catggccgcc agctgctcgg cggagagctc
     4561 gccccattga atcccggcag gcgcggcggg cggcgcctgg acgcggatcg tgcgcgtcgg
     4621 cgtgcagatc aggcttaggg gcaggtcgtt gtgggcaatg ggaaagtcct ctaccagctg
     4681 cacgtcgtgg accgtcgtgg ccaccgggac ggggtcgtgg ccgagctccc gcaggatggc
     4741 gaactccagg tcgctgtacc cggcgccttt ccccgcccgc ttgcccgacg aggtagccgc
     4801 cgcgcacccg gtcacgatgg catccagctg gggcaggtct tcgagggcta ccggccgcgc
     4861 ccagcgcagc atggtcttga gcgccgctgc ctcgcggaac cgctcggggg ggatccgcgc
     4921 cggatccagc agaatgaatc ccgctgcgag cttcggtgtc ggcacgtaaa cccggatccc
     4981 acgctgcagc gccaggaacc gaacgggcag ctggggcgag tcgggattga ccttgatagc
     5041 ccccgcctcg gaccaggggg cttgcgcaaa aatccgcatc gcggctgccg ccgcgccggc
     5101 gaagttcgga atccgcccat ggggtgggaa cggaaatctg gccagacgac gctcgtcgag
     5161 ggcatcccac gcccattgac gcgcgtccgc cttggagccg aacctggtga cgtcttctag
     5221 caaggcgagt ggtgccagcc aatacggtca cggcatacta cacggccgct cagagagatg
     5281 gcatgaccaa cttccttctg caacgctacc cggctttcgg cttcccgcgc taccgaaagt
     5341 actggctggc gtctcttgcc tccgtgggcg cgacccagct gatcaccctg gcccagggtt
     5401 ggctggtatt cgagctatca gggtcggcgc tgcagctggg aattctgggc gccgccgtcg
     5461 ccgcgccgaa catcgcgatg acgctgatcg gcggcgtgtt cgccgaccgc ttcgacaagc
     5521 gtctcattct ggtctggaca tcgggggcca tcgccacgct actcgcgctg ctggcctggc
     5581 tggactacag cggtaccgtg cgcgtgtggc acgtcctcgc cgtggcggcc gcagtctctc
     5641 tcatcaccgg gctcgactgg cccgccaggg tctccatctt tccacagctc atcgaccggc
     5701 cggctttcat gagcgccgta gcgctcaact ccatcatctg gcaggccacg cgcatggcca
     5761 tgccggcact gggcgggatc atcatagcgg ccagcgacac ctgggtgctg ttcgccctgg
     5821 gcagcggcgg ctttctgacc atgatgatcg tcatcagcgg cctgcacgtg accaccagcg
     5881 acgcgcgcca cggctcgccg ctggagcaga tcgccgaggg cgtgcgcttc atcgtcaacg
     5941 aggagctgtt caagtggctg atgctgctca cgttcatcgg catgtttttc gcgggcagct
     6001 acgtgcagat catgcccgtg ttcgcagacg tcctggagtc cggtgaggcc ggctacggtt
     6061 atctgctctc ggccggaggc gtgggcgcgg ttgccggcac gctgctggtc gggggggtgc
     6121 aggagcatgc gaggctcggg ttcgtcatgc ttggcggcgc aaccctgtcc gccgccctaa
     6181 ccgccgtgtt cgctctggtc gcagtgggtg gttggttcat cgccgcgctg ctggcgacgt
     6241 ttttcgcatc ggtcttcacc tcagtcttca tgatcagctc catggccgtc ctgcagctcg
     6301 tcgttcccga cgacctgcgc ggccgggtca tgggcatcca cgcgattggc tacagcctga
     6361 taccgttggg aggcttgttc atgggcgctc tcgccgaggc cgctgggccg gcgctggcgg
     6421 tgctgatcgg ctgttgcgtg ttcggcttcg ccatcgccgg cgtagccatg accagcacga
     6481 ccattcgcgg tctcgatggt cgcctgatcg ggcacaagca agcatgagcg aggtgggcaa
     6541 atggctggtg ctcgccggag cgttaaccgt gatcgtcggg ttgctgctgc agacggggtg
     6601 gctcggatgg ttcggcaggc tgccgggaga catccgggtg gagcgcccgg ggctctcctt
     6661 ttactttccc atcacctcga tgctcatcgt ctcggccatc ctctcggtgg ccgcctggat
     6721 cctccgacgc ttcctcggct gagcctcaac caccggcctc ggcagcgccc tccctgctct
     6781 tccgggccag ctccagctgc ttgtgcagaa cctgcagaga gctgttctga agatcacgct
     6841 gccgctcgag ctcctcgacc ttgcggtttt cctgctccag cgttttggcg agccggtcct
     6901 gctcctgaga agactcccgg agccggccct cgagctcgag gatctcgctg cgcagaactt
     6961 cctccatggc agccgacgcg ccgtgggatc cctgggcctc ctcccgcgcg gcttcgagct
     7021 gctcgccgag aacgcggacc tgctccttga gctgctcggc ttcctgttga agacgccggg
     7081 atatctttgc cagcttgtcg gcagcgttga agcggcgatc ccgcctttcg agctcgcggc
     7141 tcaacgcctc gacctctcgc tccttctgcc tgatcgtggc ggccagcttc ctcagctgct
     7201 cgtcctgggc gtcggcgcga gccgcgagct cgagatcgtc gacgcccacg gaaccgagcc
     7261 ctctgcgctc gccggaagcc ttgcctgcga gggatccgtg cagcagccac cccacaccga
     7321 cgccggcgac cagggcaacc agcagatagc cgacgatgtt gacgacgaga tagatcacgc
     7381 ggtgcctccg accacccgga attcaacccg gcgattttcc cgacgtcccg attcgcgggc
     7441 attggaagcg acgggctggg tctcgccgta gccggcagct acgagacggt ccgggcgaac
     7501 gcctctttgg accagagcct tgcgtaccgc gtccgctcgc cgctgactca gcttgaggtt
     7561 gacggcggca tcgccgaggg aatccgtgtg cacggcaatc tcgatgggcg tcgcgcatgc
     7621 gcccatggtc cgggcaagct cggcaatcaa cgggtagctg gagtcgccga tctcggcgcg
     7681 accggtcttg aaggtcacgc caagctccgc cagcgccgag tccatctgcc tctgacacgc
     7741 gccgtcggcg ccgagcacct cgatctcgtt ctggaccgaa gtgacacctg ggagcgcggc
     7801 gaccgctgct gccgcgtgaa gcctttgggc atcgcttggc gcggcacccg tcagcctgac
     7861 ccgctggccc aaggcctcca cacccgccca gtaaaggccg tgctcgcgcg cctcgccctg
     7921 ggcgcgtatg ccgacgtcgt cctggatatc tgatgcctcg ttgaacacgc aaatgaacgc
     7981 caggcaaaga aagaatgcgc cggcggctgt gaggatctcg aactgtgaga aacgaaggtc
     8041 cattttcctt acttacagga ttcgagccga gttggtccct tgggggattt tgaagcgatg
     8101 gcggctcgtt ttacgtgaac cgattctcaa cttacccgac ccgcgcgggc aaaggcaacg
     8161 ccaatccggg gccccatcgg cgcatgcccc agagcacatc ccgcgcccct cagccctcag
     8221 cggggttcgc cttgtccagg gcaagccccg ccttggccgc aaagcccagc gccacgtcac
     8281 gctcgagccg gttgttctgg gccagggacg acaacgcggc gaacgcgatc cactcggcgg
     8341 aaacttcgaa gtagccgcgc aaaacccggc gatcctccga cagcccgaag ccgtccgtgc
     8401 ccagcacggt ataggggccc ggcacccagc gagaaatgct catgggaagg gacttctggt
     8461 agtccgaggc ggccacgaat atcccttctt ccccctctag cagcgcttcg gcgtaggccc
     8521 gctcatcgcc gtcgccgaca ccgagcaggc gggctcgctc cgaggcgagc gcctgccggg
     8581 ccagctcgtt gtagctcgta acgctccaga cgttacacgc aatgccatgc tcggcaagaa
     8641 ggctctccgc ccgcaacacc tcggtcatca gcgcgccgct gccgaacagg ttgaccaccg
     8701 cccccggagc acggctgaag cagtacatgc ctttgagaac gccgtcctcc acgccttcgg
     8761 gcatggccgg catggggtag ggctggttgg tcagggtgag atagtagaag acgtcttcct
     8821 gcagctgata catccgcctg atgccctcac gaaccagcaa ggctatctcg aaggcgaacg
     8881 ccgggtcgta gctcttcagg ttgggaatcg tgttggcaac cagatgggag tgcccgtcct
     8941 gatgctgcac cccctcgccg ttcagggtgg tacgccctga agtgccgccg aggagaaaac
     9001 ccctgcacat catgtcgccc gcggcccaga tcatgtcgcc gacacgctga aaaccgaaga
     9061 tcgagtagaa gatgtagaac ggaatcatcg gcaggccata attcgcgtag gccgtgcccg
     9121 cggccaggaa agacgccatg gcgcccgttt cgcagattcc ttcctggaga atctgaccat
     9181 cctcggcctc ccgatacggc gcgatggtgc ccgcatccac cggctggtag cgctgaccgg
     9241 ccggcgagta gatgcccgcc tggggaaaaa ggccgtccat cccgaaggtg cgcgcctcgt
     9301 cgggaacgat gggcacgatg tatcggccga gagccgggtc acgcagcagg cgggacagca
     9361 tacggaccac cgccgccgtg gtggtcaccg gcctgtccga tcctttgagc atggcggcaa
     9421 aaagctccac ggacgggggc tccagcgccg cgcagcgcac ttcccgggcc ggcaggtaac
     9481 cgcccaggct atggcgccgg tcgcgcaggt agaccagctc tgcagagtcc tcggccgggc
     9541 gatagaaagc cgctcgcgat atctcctccc gggtgagcgg aatgccaagc ctcttcgcga
     9601 gctcctgtcg ctcctgctcg ttgagctgct tcttctggtg caccgtgttg ctgccttcgg
     9661 tgcctggccc gagaccgtcg cccttcaccg tcttgagaag caccacgcac ggcttgccgc
     9721 gaacgttggc cgcccgggca aacgcggcat agatcttctg gtgatcctgg ccgccacgct
     9781 tgatggaccg gatctcctcg tcggagagcg tcttcatcag ctcctcgagc tcgggggatg
     9841 tttccaccca gtgctcgcgg accacgtcgc cgctggacac cgtgtacatc tgatagtcgc
     9901 catccacggc ctgctccata cgctcctgga gaatgccgtg ctcgtcccgg gcaagaatgg
     9961 catcccagcc gccgccccag atcaccttga tcacgtgcca gtcggcgccg cgaaaggcgc
    10021 gctcgagctc ctgaatgatt tttccgttgc ccctgaccgg gccatcgagc cgctgcagat
    10081 tgcaattgat caccagaacc aggttgtcca gccgctcccg gctggcgatg ttgatggtgc
    10141 cgagcacctc cggctcgtcg gactctccgt ccccgatgaa cgtccagatc ttgccgccgt
    10201 tgttggcctt gagcccgcgg ctttccagat atttggcgaa ccgtgcctgg tagatggcgc
    10261 tgggcgtaga gagccccatg ctggccgtgg gcatctgcca gaaccacggc aggcggcgcg
    10321 gatgagggta ggagggcaga ccaccaccgg gcgccagctc gcggcggaag ttggtcaacg
    10381 cctgctcgga aagacgccct tccagaaacg ccctggcata gacgccgggc gaggtgtggg
    10441 cctgaaagtg cacctgatcg ccgccgtagg attcggacgc gcctcgaaag acgtgattga
    10501 aacccacttc catcatcgtc gcggcggaca ggtaggtggc gatgtgacca ccgacccccg
    10561 agccgctgtc gtaggcctgc agcaccatgg cgaccgcgtt ccagcagatg atgtcgccga
    10621 tcttcatctc gagctcgatg ttgcccgggt aggcgggctg atcgcgcacg tcgatggtgt
    10681 tcttgtacgg ggtgttgagc gcggcgtcgg tcagaatcat gccgcgatga ctgacatggg
    10741 cctgcagccg ccgaagcagc tccttgacct cgtcctcacc cctgaaggcg agcacgtcgt
    10801 caatggcgtc gcaccattcc tgctgctccg acagccaatc gtcggggtta cgctccgcca
    10861 tctccgtcac cgtacccaat gtcttaggca agcggggaag ctacgccatg gggcaagcgc
    10921 ggtaaaccgc tccgagaaac ggtcgctgag ccgggaacgg gatcttgggc gagaatgcgg
    10981 ccggacgagc acgccagcct actgctgaat cgtaacgggc tccccgaggc gcagggtgac
    11041 cgcgatcggt cgatgatcgg agagccggca gtccagaacc tcgtagtcgt cgatcgacag
    11101 ccccggcgag accagcacgt gatccagagc aaacgcaggc tgccaggcgg gataagtggg
    11161 gtgcacctga tccgccggca ccagatccgt ttccgaaagc ggcgagttga agagcagctg
    11221 ggcaagatga ctgttcatgt ctcccatcac caccacgtgc tcttcggcgt cgatcaactc
    11281 gcgcacatac tcgagctgca cctggcggga acgcccgccc aaagagaggt gcagaaccac
    11341 cagcgagacc gtcgactcgc cgagcttgaa gcgcatcagg atcgcgccgc gtcccggaat
    11401 ggcgcccggc agcttgtggt cttccatgtg cacgggcgcg attcgcgaca gaaccccgtt
    11461 gccgtgctgg gcgaacggac ccaggtcacg gttgcactgg gtgtaccagt aaggaaaacg
    11521 cgcgcgatcg gcaatgtact ccacctgatt gatgaagccg ctgcgaaggc tgccgccgtc
    11581 gatttcctga agcgcgacca gatcgtagtc gcgcaccaga tcggcgatgt ggcggatatt
    11641 gatggcccgc gcttcgtgag gcagcacgtg cttccagccc ttggtgacgt agtggcggta
    11701 cttggtggtc cgaataccca cctgaatgtt gaaggtgagg agcctgagcg aatgcgccgc
    11761 ctcctcggga gccacatcgg actccaagtg catgttcacg accggggtga cagccgtact
    11821 acggttctcg ccgcgcctag accaagccaa catggcggac tgctccatcg acttggtcat
    11881 gagtctagcc ttgcgctgcg gattggactg cgtactaact aacatttcgt tcgatcacga
    11941 ggccccagaa gtggccgcgt cctcttcctc aacggacgcc ggctccgggg cggcgatgcc
    12001 acgcgccgct cgctgctttg cgacaaggtg gttgaccact tccagcatgg cggcgttgct
    12061 gcccgccatc cgaccgtcca cgcgataggt gccatccacc accagggcgg ggaccccgga
    12121 gacgcgataa gcccgggcat gggccttggc ctgctcgatc cggctgcgaa ccccgaagga
    12181 gttgaagacc tgagcgaact gctgggggtt cacgccggcg acttgctgga acagggcggc
    12241 aatggcgcca ggttcgcgca ggtcccggcc ctcttcgtgc accgcccgaa agatggaatc
    12301 gtgcacggcg ccgccgagat ccaggatctg ggcagtgtag aacgctttgg cgagggtttc
    12361 ccactcggcg ctgaaaatcg cgggaacgcg cctgaaaccc acgtcctcgg gctggttctc
    12421 tcgccaggct tccaacgccg ggtcgaaggt cttgcagtgt atgcaggcgt aggagaatac
    12481 ctccaccacc tctaccctgg cggcgtctgc cgtggccacg ctcaccggca gccgctgata
    12541 gtgaacgccc tcttgccatt cctgggcaaa ggcggggccc gttgccccga gggcaaccac
    12601 tagcaggaaa gcgagcacgt ctctcatttc tacctctctg gaggactctg ttcggccccg
    12661 accatgaccc tgcggcgacg ggtaggcctc gtccccgcca tgcgcgattc gaaccgaggg
    12721 cgccttggga accactcgtt ggacaacgtc cggttcgctt agtgcagccc ctgaagatag
    12781 tcagccaggg ccgcgatctc accatcggtc aggccgccgg ccacgccacg catcatgccg
    12841 ccgtatacct cgtcggtctg tcggcgacct tcccgatagg ccgtgagctg ggcgatggtg
    12901 taactacccg cctgcccgcc aacggcggga aagccggcag aggcgttgcc ttcgccgctc
    12961 ggcgcatggc aagcggcgca ggccgccaca cctttcttgg cgatcccgcc gcgatagatc
    13021 tgctcggcga gcgcaatgga gtcgtcgtct ccctcagcct ggcccagctt ggcgggcaac
    13081 gacgcgtagt atgccgccag atcctgcagg tcctggtcgc tcttgccgat gagctggccc
    13141 gtcatgagaa ggatcttgcg gtcgtctttc tggatcatga cgagctggtt gtatagatac
    13201 ttctcgttct ggccggccag gttcgggtag ctgggatcca cacccgacgc accgtcctga
    13261 ccatggcagg cggcacagac cacggcctga gcagcccctg cttcgggatc acccgcggc
//
LOCUS       DBNHQR010000160         2262 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1154731, whole genome shotgun sequence.
ACCESSION   DBNHQR010000160 DBNHQR010000000
VERSION     DBNHQR010000160.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1154731"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..807
                     /locus_tag="AC16VR_03625"
     CDS             13..807
                     /locus_tag="AC16VR_03625"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6010277.1"
                     /translation="MSDLNGKVAIVSGAAQGLGEAFARALSEQGAAVLAFDVQETIGD
                     VAAAIEGATGNRVLGFVADVSKRDDVERVVAATVETFGGLDLLVNNAGNWRRTPVDAS
                     WEQALADWDFIMDTNLKGLLMLSRACVPHLQSRGGGDIVNISTYYVLPAKSEGTNSPE
                     TDLYNASKWAINGFTDAWAKYLAKDNIRVNGMCMGATDTPMLRGLFDDGQLPPDLAET
                     VMRPAQIAQQMIDLLADGRSGENIGAWVGEPIVIPPHPPEHRRISG"
     gene            complement(816..1715)
                     /locus_tag="AC16VR_03630"
     CDS             complement(816..1715)
                     /locus_tag="AC16VR_03630"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6010278.1"
                     /translation="MEFFANYPASTGVKPDVWAREKEAQGWHGICASDHLWVGATRYP
                     HVFVAATQMACATTRTLITTSFCNNLFRSPVEFAQAALSLQAASGGRFEAGLGAGWLQ
                     DEIEAMGDVYPSGPERVSRYVEALTVVRSLLATGQCDFQGDHYQVRIQGEHALGLVTD
                     SPPPLIASAGGPRALREVAPLVDRVEVKASARATRVGYLDFEIMATVTEEEVRGQVER
                     VRAVNADVPLGIFILTGTGDNPAVKGLKAALANGYLSRFVGEPEQVAEALNQLGEIGI
                     DRVQLTELVPGSHAELAPYLLSG"
     gene            1865..>2262
                     /locus_tag="AC16VR_03635"
     CDS             1865..>2262
                     /locus_tag="AC16VR_03635"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6010279.1"
                     /translation="MQAADWIDLSGKVAHVTGAGKGIGAGIARALAMAGATLAVSDVD
                     GEAAARTAEEIGGLGMQLDVSDRAAVDAALERTADELGALDILVNNAGVYIGFGGPVR
                     EITDEMWRTLWSINVDGLFYCCRAAARIMI"
BASE COUNT          379 a          735 c          788 g          360 t
ORIGIN      
        1 aggagagtct caatgtctga tctcaacggt aaagtcgcca tcgtcagtgg tgccgcccag
       61 ggtctaggcg aagccttcgc ccgggcgctg agcgagcaag gtgcggcagt gctcgccttc
      121 gacgtgcagg agaccatcgg cgacgtggcg gccgccatcg agggagcgac gggcaaccgg
      181 gtactcggat tcgtggccga cgtctcgaaa cgcgacgacg tggaacgggt ggtagccgcc
      241 accgtcgaga ccttcggcgg cctggacctg ctggtgaaca atgccggcaa ctggcgccgc
      301 acgccggtgg atgcgtcctg ggagcaggcc cttgcggact gggacttcat catggacacc
      361 aacctgaagg ggcttttgat gctgtcccgt gcctgcgttc cgcaccttca gtctcgcggc
      421 ggcggcgaca tcgtcaacat cagcacctac tacgtgctcc cggccaagtc ggagggcacg
      481 aactcgccgg agacggatct ctacaacgcc tcgaagtggg ccatcaacgg cttcacggat
      541 gcctgggcga agtacctggc caaggacaac atccgggtga acggcatgtg catgggcgcc
      601 acggacacgc cgatgctgcg cgggcttttt gacgacgggc agctgccgcc ggatcttgcc
      661 gagacggtga tgcggccggc ccagatcgcc cagcagatga tcgatctgct cgccgacggc
      721 cgctcaggtg agaacatcgg tgcctgggtg ggcgagccca tcgtcatccc gccgcacccg
      781 cccgagcatc ggcgcatttc cggctgagcc ggcgctcagc cggagagcag gtagggcgcg
      841 agctctgcgt gggagccggg caccagctcg gtgagctgga cccggtcaat gccgatctcg
      901 ccgagctggt tcagagcctc ggccacctgc tcaggctcgc ctacgaaacg ggaaaggtac
      961 ccgttcgcca gcgctgcctt cagccccttc acggcgggat tgtcccccgt gccggtgagg
     1021 atgaagatgc cgagcggcac gtcggcgttc accgcgcgga cgcgctcgac ctggcctcgc
     1081 acctcttcct cggtgaccgt cgccatgatc tcgaagtcca ggtagcccac ccgggttgcc
     1141 cgggcgctgg ccttcacctc gacccggtcc accagcggcg ccacctcgcg cagcgcgcgg
     1201 gggccgccgg cggaggcgat cagaggcggc ggagagtccg tgacgagccc cagggcgtgc
     1261 tcaccctgaa tccgcacctg gtagtggtcg ccctggaagt cgcactggcc ggtggcgagc
     1321 agagaacgaa cgacggtcag tgcttccacg tatctggata cccgctcggg acccgacggg
     1381 tagacgtcgc ccatggcctc gatctcgtcc tgaagccagc ccgctccgag acctgcctcg
     1441 aaccgcccgc cggatgcggc ctgcagtgag agggccgcct gggcgaactc caccggcgag
     1501 cggaacaggt tgttgcagaa cgacgtggtg atcagcgtgc gcgtggtggc gcaggccatc
     1561 tgagtggcgg cgacgaacac gtgggggtag cgcgtcgcgc ccacccagag gtgatcgctc
     1621 gcgcagatac cgtgccagcc ctgtgcttcc ttctcccggg cccacacatc gggcttcacc
     1681 ccggtgctcg cggggtagtt ggcgaaaaat tccatatggc ggtttactcc ctgcacccgt
     1741 tctgctcgtc ccgggcgctg cccgcgacaa tactaaccca aaggatcggg acactcgtcg
     1801 tcgcggccgg agcagccggg atgatgtgct acagtctcgc taagaatgac gacgcggggt
     1861 gggcatgcag gctgcggact ggatcgatct gagcgggaag gtcgcccatg tgaccggcgc
     1921 cggtaaaggc atcggtgcgg gcatcgcgcg ggcgctggcc atggcggggg ccacgctggc
     1981 cgtgagcgac gtggatggcg aagcggcagc tcggaccgcg gaggagatcg gcgggctcgg
     2041 catgcagctc gacgtgagcg atcgtgcagc ggtggacgcc gctctcgagc gcacggctga
     2101 cgagctcggc gcgctggaca tcctggtcaa caacgccggc gtctacatcg ggttcggggg
     2161 acccgttcgt gagatcaccg acgagatgtg gcgcacgctg tggtccatca acgtggacgg
     2221 gttgttctac tgctgccgag cggccgcgcg gatcatgatc ga
//
LOCUS       DBNHQR010000161         1759 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_78804, whole genome shotgun sequence.
ACCESSION   DBNHQR010000161 DBNHQR010000000
VERSION     DBNHQR010000161.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_78804"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="AC16VR_03640"
     CDS             complement(<1..206)
                     /locus_tag="AC16VR_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HIR6010280.1"
                     /translation="MDTDLATQVEQLADRVRGLSSASVDKPVLVYFDIIGICWPIRCL
                     LHLKGVDYELVQVPIQAWAFRDANG"
     gene            286..1035
                     /locus_tag="AC16VR_03645"
     CDS             286..1035
                     /locus_tag="AC16VR_03645"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIR6010281.1"
                     /translation="MTEELQQAHEALIRGEGFTVLSSLMTEGEAARVRDSILNHLEQA
                     VDERDGVARLSNLLAVDTSFDAYVTNPMLLGLAHRMLGPDARLAALGARVLMPGCELG
                     GLHVDYPYWAMDPGMPVEPALMLQVIWMLEPFTEDNGGTWVAPGSQAWGEYPSEKRFA
                     DSAVQITGNAGDAVVSHGLLWHRTAKNRTDEPRVALLINYSQLAIQPMTPMGPFTDEY
                     RAGASDELRALLGFDHGKALRHRAMNVAREG"
     gene            complement(1032..>1759)
                     /locus_tag="AC16VR_03650"
     CDS             complement(1032..>1759)
                     /locus_tag="AC16VR_03650"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR6010282.1"
                     /translation="DSRAHYGGRMVFDEAGYLYFSVGDRGSRDLFPQDLTRDAGKIYR
                     IHDDGRIPEDNPFLHREDAKPAIYAYGLRNPQGMARHPGTGKIWIHEHGPRGGDELNV
                     IEPGANYGWPILSHGVNYSGSEFAEGTEREGFRSPAWHWTPSIAPSGMAFVTSPRYPE
                     WRGGLLAGSLKFGYLVLCRLDGDRVTGQEIVVEDLGRLRDVREGPDGFIYVAIDGRGI
                     VRILSSVGAGESAADGAETIPGL"
BASE COUNT          303 a          597 c          567 g          292 t
ORIGIN      
        1 ccattcgcgt cgcgaaaggc ccaggcctgt atcggcacct gcaccagctc gtaatcgacg
       61 cccttcaggt gcagcaggca gcgtatgggc cagcagatgc cgatgatgtc gaagtagacg
      121 agcacgggct tgtcgacgct tgcgctggac agcccgcgaa cccggtctgc aagctgctcg
      181 acctgcgtgg cgagatcagt atccatggcc cgttcccctt cccttgcttt ggagcgaagc
      241 ataccccatg ccgtgctagt ctctcccctg tcagggggct cgaaaatgac cgaagagctg
      301 caacaggcgc acgaagcgct gatccggggc gaaggcttca cggttctctc cagcctgatg
      361 acggagggcg aagcggcccg cgttcgggac tcgatcctca atcatctgga acaggccgtg
      421 gacgagcgcg acggggtcgc gaggctttcg aacctgctgg cggtggatac gtccttcgat
      481 gcgtacgtta ccaatcccat gctgctggga ctcgcccacc ggatgctcgg ccccgacgcg
      541 cggctggccg cccttggggc ccgggttctg atgccgggct gcgagctcgg cggcctgcac
      601 gtggactatc cttactgggc catggatccc ggaatgccgg tggagccggc cctgatgctc
      661 caggtgatct ggatgctcga gcccttcacc gaggacaacg gcggcacctg ggtggcgccc
      721 ggcagccagg cctggggcga gtacccgtcg gagaaacgtt ttgcggattc cgccgtgcag
      781 atcaccggca acgcgggtga cgccgtggtg agccacggtc tgctgtggca ccgaacggcc
      841 aagaaccgga cggacgagcc cagggtggcc ctgctcatca actactccca gctcgccatc
      901 cagcccatga cacccatggg ccccttcacg gacgagtacc gggcaggcgc atcggacgag
      961 ctgcgggcgc tgctcgggtt cgaccacggc aaggccctgc gccatcgggc catgaacgtc
     1021 gcccgggagg gttagagccc cgggatcgtt tccgctccgt cggccgccga ctcgccggcg
     1081 ccaacggacg aaaggatccg gacgatgccc cgaccgtcaa tggccacgta gatgaaacca
     1141 tccggccctt cccggacgtc gcggagtcga cccagatctt cgacaacgat ctcctgcccg
     1201 gtcacgcgat caccgtccag ccggcacagc accaggtaac cgaacttcag cgagccggcc
     1261 agcaggccac cccgccattc gggatatcgc ggcgaggtca cgaaagccat gccggagggc
     1321 gcgatggacg gcgtccagtg ccaggccggt gagcgaaacc cctcccgctc ggtgccttcg
     1381 gcaaactcgc tgccgctgta gttcacaccg tggctgagga ttggccaccc gtaattggcc
     1441 ccgggctcga tcacgttgag ctcgtcgccg cccctgggac cgtgctcgtg aatccagatc
     1501 ttccccgtcc cgggatggcg ggccatgccc tggggattgc gaagcccgta ggcgtagatg
     1561 gccggcttcg catcctcccg atgcaggaac gggttgtcct cggggatccg gccatcgtcg
     1621 tggatccggt agatcttacc cgcgtcccgg gtcaggtcct ggggaaacag gtcccggctg
     1681 ccccggtctc ccacggagaa gtacagatat cccgcttcat cgaagaccat gcggccgccg
     1741 tagtgggctc tcgaatcgc
//
LOCUS       DBNHQR010000162         4001 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1598934, whole genome shotgun sequence.
ACCESSION   DBNHQR010000162 DBNHQR010000000
VERSION     DBNHQR010000162.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4001)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1598934"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..113)
                     /locus_tag="AC16VR_03655"
     CDS             complement(<1..113)
                     /locus_tag="AC16VR_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+)-trafficking protein"
                     /protein_id="HIR6010283.1"
                     /translation="MSRTVTCRKYGEELEGLDAPPMPGPKGQEIFETVSRRA"
     gene            complement(110..1177)
                     /gene="mutY"
                     /locus_tag="AC16VR_03660"
     CDS             complement(110..1177)
                     /gene="mutY"
                     /locus_tag="AC16VR_03660"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014578252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HIR6010284.1"
                     /translation="MRPDWFTKRVLKWFDLHGRHDLPWQEDNDPYRVWVSEIMLQQTQ
                     VTTVIPFYERFIARFPTVAALAGAELDEVLHHWTGLGYYARGRNLHKAARVIAHDLDG
                     RFPVGVEALSELPGIGRSTAGAISAIAQGVRAPILDGNVKRVLARFHAVAGYPGQTAT
                     QKSLWELAEAHTPNTRVADYTQAIMDLGATVCTRQPRCEVCPVAERCDALAQGATDDF
                     PGRKPKRSKPTRTARMFLIVAPDGACLLEQRPAKGLWGGLWIPPQRDPDCDVPAVLRE
                     FDIPESAVAESRVEPGFRHTFTHFHLDIEPVHVRLRARPLPGVRDGTDCLWYRREDDT
                     EVGLSAPAVALLDSLKEFELA"
     gene            complement(1180..2694)
                     /locus_tag="AC16VR_03665"
     CDS             complement(1180..2694)
                     /locus_tag="AC16VR_03665"
                     /inference="COORDINATES: protein motif:HMM:NF017021.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HIR6010285.1"
                     /translation="MVKRILMILLWIVLLGGGFVFYWIQDANRIKPELEQLIEEQAGI
                     PVTIGGDLSWRLFPPLTLQAEQIAASYEGQDWTAERLSLDIDVMTVLRTRDVNRWRVQ
                     SLTLTNTVGRDARTEIDIKSLTLREFELGKPSPLEISMVYTGEGAEPLPVKMTGLVTV
                     QSGPDRYEITNADLSTPYADGVCNLEASPASSSAPLPPATLEDLIPVATWRAFDWSGR
                     CDLSRLALDDLTFDSATVDLANTAANSATRITIPEFFGGMAILELQIDASGTPVRWIL
                     TPDLTGVDSQRLMAWLDQRLEWMAPLAYNGTLKFEGNTADALIASLSGETRFDGGRGA
                     ISIIKIKQPLLAVATMLQEPGRVSGWPDLWQYDRLEGVWRADRQHHTLDFALDNLSVI
                     ADGNYDPAADRLDMMAELTFRTLTEGKMFELNPVLMDLPIPVRCRGSLENPECRVDQK
                     AAQRIVASVLTSKEGNEAREKLEEKIDEEVPEEYRDAARGLLDLLGRSLQDKNQ"
     gene            2806..3204
                     /locus_tag="AC16VR_03670"
     CDS             2806..3204
                     /locus_tag="AC16VR_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010286.1"
                     /translation="MTVESFDPSAVDHTISDSRLARLLDAAGCLDEESFGLGTEEVRD
                     LAAVARHPESDWSERAGTLGDEEVLALIRLFTLAETRLSGWESGAASPVVPLAAALKR
                     RDAYPSDLNRWIRSNTANRFLPYGSLMDRL"
     gene            3263..3874
                     /gene="hisB"
                     /locus_tag="AC16VR_03675"
     CDS             3263..3874
                     /gene="hisB"
                     /locus_tag="AC16VR_03675"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HIR6010287.1"
                     /translation="MATKTKPSGRSVTVSRDTQETRIAVSVDLDGSGASRLTTGLAFF
                     EHMLTQIARHGLIDLDIRADGDLEVDAHHTVEDVGIVLGQAIGQAVGDKAGISRYGHA
                     YVPLDEALSRAVVDFSGRPGLTYEVDFVRPRIGDFDVDLLREFFQGFVNHAQVTVHLD
                     NLRGENAHHQAETLFKAFGRALRMAVAPDPRMAGQVPSTKGSL"
     gene            3875..>4001
                     /locus_tag="AC16VR_03680"
     CDS             3875..>4001
                     /locus_tag="AC16VR_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HIR6010288.1"
                     /translation="MLLIPAIDMKDGQCVRLRQGRMDDATVFSDDPLAMAAHWAEQ"
BASE COUNT          655 a         1262 c         1350 g          734 t
ORIGIN      
        1 gctctgcgcg agacggtttc gaagatctcc tggcccttgg gtccgggcat aggcggcgcg
       61 tcgagaccct cgagctcttc gccgtacttg cggcaggtga ccgtgcggct catgccagct
      121 caaactcctt aagggaatcc aatagtgcaa ccgcgggagc ggacagtcca acctcggtgt
      181 cgtcttctct ccggtaccac aggcagtccg tgccgtcgcg aacgccggga agcggtctcg
      241 cgcgaaggcg gacgtgcacg ggctcgatgt cgagatgaaa gtgggtgaac gtgtggcgaa
      301 acccaggctc gacccggctt tcggcaacag cggattccgg gatgtcgaac tcccggagca
      361 ccgctggaac gtcgcagtcg gggtcccgct gcggcgggat ccacaatccg ccccacaggc
      421 ccttggccgg tcgctgctcc aacaggcacg ccccgtctgg ggcgacgata agaaacattc
      481 tcgccgtgcg cgtcggcttg gaccgtttcg gcttgcgccc gggaaagtca tcggttgcgc
      541 cctgggcgag ggcatcgcag cgctcggcca cggggcagac ctcgcaccgg ggctggcgag
      601 tgcacacggt agcgccgagg tccatgatcg cctgggtgta atcggcgaca cgcgtgttcg
      661 gggtgtgggc ctcggcgagc tcccagagcg acttctgcgt ggccgtctgg ccggggtagc
      721 ctgccacggc gtggaagcgg gctagcaccc gtttgacgtt gccgtcgaga atcggcgcgc
      781 ggactccctg ggcgattgcg gagatggcgc cggcggtaga acgaccgatt cccggcagct
      841 cgctcagcgc ctcgaccccg acgggaaacc ggccgtcgag atcgtgcgcg atgacccggg
      901 cggccttgtg gaggttgcgg ccccttgcgt agtagccgag ccccgtccag tggtgcagga
      961 cctcgtccag ctccgcccca gcgagtgctg cgaccgtcgg gaaacgggca atgaaacgct
     1021 cgtagaacgg gatcacggtg gtgacctggg tctgctgaag catgatctcg gagacccata
     1081 cccggtacgg atcgttgtcc tcctgccacg gcaggtcgtg ccggccgtgc aggtcgaacc
     1141 acttcagaac gcgtttcgtg aaccagtcgg gcctcaccgt tactggttct tgtcctgcaa
     1201 tgatctgccc aggagatcca gcaggccgcg ggctgcgtcc cggtactcct cagggacctc
     1261 ttcgtcgatc ttctcctcga gcttctctct cgcctcgttg ccctctttgc tcgtcagcac
     1321 ggaggccacg atgcgctggg cggctttctg atctacccga cattcggggt tttccaggct
     1381 gccgcgacag cgaacgggaa tgggcaggtc catcaggacc gggttcagct cgaacatctt
     1441 tccttcggtc agcgtcctga aggtcagctc cgccatcatg tcgagccgat cggcggcagg
     1501 atcgtagttg ccgtctgcga tgaccgacag attgtccaga gcgaagtcca gggtgtggtg
     1561 ctgccggtcc gcccgccaca cgccctcgag acgatcgtat tgccacagat cgggccatcc
     1621 ggagacccgg cccggctcct gcagcatggt ggcgacagcc agaagcggct gcttgatctt
     1681 gatgatggag atggcgcccc gcccaccgtc gaagcgcgtc tcgccggaaa gcgaggcgat
     1741 gagcgcgtca gccgtgttgc cttcgaactt cagggtgccg ttgtaggcca gcggcgccat
     1801 ccactccagg cgctgatcca gccaggccat gagacgttgg ctgtccaccc cggtaaggtc
     1861 gggggtcagg atccagcgaa cgggcgtgcc gctggcgtcg atctgcagct cgaggatggc
     1921 catgccgccg aagaactcgg gaatggtgat ccgcgtggcg ctgtttgcag cggtgttggc
     1981 gagatcgacc gtggcggagt cgaaggtgag gtcgtcgagc gcgaggcgcg acaggtcgca
     2041 tcggccggac cagtcgaagg cgcgccatgt ggcgacggga atgagatcct cgagggtcgc
     2101 cggtgggagc ggggcgctgc tgctggccgg gcttgcctcg aggttgcaga cgccgtcggc
     2161 gtagggagtg gatagatccg cgttagtgat ctcgtatcgg tcgggtccgg actggacggt
     2221 aacgagcccg gtcatcttca cgggcagcgg ctcggctcct tccccggtat agaccatcga
     2281 gatctccagc ggcgacggct tccccagctc gaattcccgc agggtgaggg atttgatgtc
     2341 tatctccgtc cgggcgtcgc ggccgacggt gttcgtcaaa gtcagcgact gcacccgcca
     2401 gcggttcaca tcccgggtac gcagcacggt catgacgtcg atgtccaggc tgagcctttc
     2461 agccgtccag tcctgaccct cgtagctcgc ggcgatctgc tctgcctgca gtgtcagggg
     2521 cgggaacaga cgccagctca gatccccgcc gatggtgacg gggatgccgg cctgctcttc
     2581 gatgagctgc tccagctccg gcttgatccg gttggcgtcc tggatccagt aaaagacgaa
     2641 gccgccaccg agcagcacga tccacaggag gatcatgaga atgcgtttga ccatgtggtg
     2701 ttgccgtgag tctgggaagt gaggacgatt ataaggattt cgggctggcc ggcagctgaa
     2761 cggcgcgttt gctatagtct cgcccccgca gaccatctgg ccgccatgac cgtagaatcc
     2821 ttcgacccct cagccgtgga ccacacgatt tccgattcgc gtctcgcgcg cctgctcgat
     2881 gcggccggtt gcctcgacga ggagagcttc ggtctcggta ccgaggaggt gagagatctc
     2941 gcggccgttg cccgccatcc ggagtcggac tggtccgagc gggcggggac gctgggagat
     3001 gaggaagtcc tggccctgat caggctgttc acgctggcgg aaacccgcct gtcgggctgg
     3061 gagtccggag cggcatcgcc cgtggtcccg ctggcggcgg cgctcaagcg ccgcgatgcc
     3121 tacccgtcag atctcaaccg gtggatcagg agcaacacgg caaaccgctt tctgccctac
     3181 gggagcctga tggaccggct gtagtcgtcg ggttcgagtc cgtatacttg cgcggcctca
     3241 ctgaagcgga aggcggttga tcatggccac gaaaaccaag cccagcggtc gctcggttac
     3301 cgtctcccgg gatacccagg agacgcggat cgccgttagc gtggatctgg acggctcggg
     3361 cgcgtccagg ctgaccacgg ggctcgcctt tttcgagcac atgctcacgc agattgcccg
     3421 tcatgggctc atcgatctcg acatccgcgc tgacggcgac ctcgaggtgg atgcccacca
     3481 cacggtggag gacgttggca tcgtcctggg ccaggccatt ggtcaggccg tcggcgacaa
     3541 ggccggcatc tcgcgctacg ggcatgctta cgttcccctg gatgaagcgc tctcccgtgc
     3601 cgtggtggac ttttccggcc gcccgggcct gacctacgag gtggatttcg tgcggccccg
     3661 aatcggcgat ttcgacgtcg acctccttcg cgagtttttc cagggattcg tcaaccacgc
     3721 tcaggtgaca gtgcatctgg acaatctgcg cggggagaac gcccaccatc aggccgaaac
     3781 gcttttcaag gcgttcggcc gggcgctgcg catggccgtc gccccggatc cgcgcatggc
     3841 cgggcaggtg ccgtccacca aaggcagcct ctagatgctc ctgatcccgg ccatcgacat
     3901 gaaagatggc cagtgcgtga ggcttcgcca gggtcggatg gacgacgcca cggtgttctc
     3961 ggacgatccg ctggccatgg ccgcgcactg ggccgagcag g
//
LOCUS       DBNHQR010000163         7109 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1662118, whole genome shotgun sequence.
ACCESSION   DBNHQR010000163 DBNHQR010000000
VERSION     DBNHQR010000163.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7109)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7109)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7109
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1662118"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..547)
                     /locus_tag="AC16VR_03685"
     CDS             complement(152..547)
                     /locus_tag="AC16VR_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HIR6010289.1"
                     /translation="MNNTHSLAVGYILWIFGFLGAHRFYFGRPVSGTIYFFTLGLFLI
                     GWLIDLILMPNLEASADRRYQQGEKSYTVAWVLLTFLGFFGVHRFYQGKWMTGIIYLF
                     TVGLFGIGVIYDYWTLNEQVSALNHERAG"
     gene            complement(722..937)
                     /locus_tag="AC16VR_03690"
     CDS             complement(722..937)
                     /locus_tag="AC16VR_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010290.1"
                     /translation="MRISQLVLKLPIPSYLNRSFYPEGFARQIAGIAIAAAGNRWPAP
                     EQLDVAAFLVHGSDDPLVPVEGGVDTH"
     gene            1014..1970
                     /locus_tag="AC16VR_03695"
     CDS             1014..1970
                     /locus_tag="AC16VR_03695"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6010291.1"
                     /translation="MQQLPDPVRIPTNDIELEVFEQGAGAPVVLCHGWPEHAYSWRHQ
                     MSPLAEAGYHVIVPNQRGYGHSSRPQKVTDYDITHLAADLTGLLDHFGYDRAVFCGHD
                     WGAIAVWNLAMLHPDRVAGVINLSVPLLRRGPTDWVSHWESLLGEDFYIVHFNRRPGV
                     ADAVFEENPRRFLGNLYRTHQWQEPAAEPRSGMPLINLARANRPRGRPLMSDAELDVF
                     VRAFEHSGFSGGINWYRNFARNWKLTADVPEHVPHPAMMIYGRYDIVPVFDKLRDFVP
                     LVETHVLDCGHWIQQELPGETNRLMLDWLERNYPSNRRRSSQ"
     gene            1967..2455
                     /locus_tag="AC16VR_03700"
     CDS             1967..2455
                     /locus_tag="AC16VR_03700"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6010292.1"
                     /translation="MNAIKGLNHAAYRCRNSEETRQFYEDFLGLPLVDALPIGTTKTG
                     RAAQVLHTFFAMDDGSCVAFFEEPDAAFDFKDQRDFDLHLALTVDMETLHEMFERGKA
                     QGIETRGISDHGFIHSIYFRDPNGYVVELAAPVELRSGASADERRLRARQVLDEWEAR
                     RP"
     gene            complement(2514..2912)
                     /locus_tag="AC16VR_03705"
     CDS             complement(2514..2912)
                     /locus_tag="AC16VR_03705"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR6010293.1"
                     /translation="MSLESRANRCFVCGPGNPIGLHVRFRLEEDVCLAEFTPGEEHVG
                     YDKVAHGGIIFSLLDDVMANWIWLRGEQCFTARADVRYRAQLPVGTPVRLEGRCLKRK
                     GRLAIMEGKVIRTDTEEVVAEASGSFMIPS"
     gene            2956..3564
                     /locus_tag="AC16VR_03710"
     CDS             2956..3564
                     /locus_tag="AC16VR_03710"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6010294.1"
                     /translation="MHDLNLIRSRLSEHRAEEFAPEPDMREAAVAVILREVSSRPAEI
                     LFIKRAEKPGDPWSGHMAFPGGHRDPEDESLRAAAERETLEEIGLDLDSARYLGPLNQ
                     QRAVARGRPLNMLIAPHVFEIAGDPSFTINYEVAQVVWTPLAPLVSGALHDTETRPLA
                     GTPTVFNGYRLPQGHFVWGLTYRMVKSFFAAIDPGWEPPPEV"
     gene            complement(3581..4969)
                     /locus_tag="AC16VR_03715"
     CDS             complement(3581..4969)
                     /locus_tag="AC16VR_03715"
                     /inference="COORDINATES: protein motif:HMM:NF012242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HIR6010295.1"
                     /translation="MRVGVGIDFGTTNSAAATYDGERLQLVALEGADPIMPSATYIDR
                     ELQTLTGQAAINQYIADNTGRTVELVPEVIAETSAFVEHGDSENPAPVETVTQKVYGQ
                     PLIDSGLHGRLFRGTKRLLGDPRVRRLMVFDHPFRLVALITPILLRMRKALSRTVSGA
                     DYAHLGHPVNFEGRDEFRNQTALARLGEAYKYAGITEQRFYPEPIAAAVSYLFAHPEA
                     AGEHLLTLDFGGGTLDFCILRRAAGDFEVVTTHGIALGGDHIDQRLFRELLFPLLGKG
                     ETWRRRGMDREIETVFPFEDYQELLVNWAVTYTLNQNRYTTPVLECIGQGGPGARKFR
                     RLRELIQQNLSYVVFQTIKDFKADLSAREEAVLDISEIDVEVRLTRAGFESLIGDLLE
                     QAEQALDETVALAGLVHGDVDIVLCTGGSSLIPAVRRILEDRFPGRVVEHDPFTSVAA
                     GLAIANFHGMDV"
     gene            complement(5039..5422)
                     /locus_tag="AC16VR_03720"
     CDS             complement(5039..5422)
                     /locus_tag="AC16VR_03720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HIR6010296.1"
                     /translation="MGTSLDDARYFNLATFRKNGSAVETPVWFAPSAGEAPGVYYVFS
                     AGDAGKVKRLRRDQTARVAACDVKGKLQGEWLQARAELIEGADAQAALVALRLRYGWQ
                     MRLADLLSRLTGRFRRRAYIRVTLA"
     gene            complement(5467..6120)
                     /gene="msrA"
                     /locus_tag="AC16VR_03725"
     CDS             complement(5467..6120)
                     /gene="msrA"
                     /locus_tag="AC16VR_03725"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 - L-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HIR6010297.1"
                     /translation="MAWEQKLRIPSESEALPGRAEEMPVPAAHHVNGAPLKEPFPEGL
                     AQAVFALGCFWGAERCFWNTPGVYSTAVGYAGGSTPNPTYEEVCSGLTGHTEVVLVVY
                     DPQKVSYAELLKVFWESHDPTQGMRQGNDVGTQYRSAIYTFDDEQAAAARASADEYQK
                     ALSAAGFSEITTEIRSAPAFYYAEPYHQQYLAKVPNGYCGLGGTGVGCNVAPLVKAV"
     gene            6254..>7109
                     /locus_tag="AC16VR_03730"
     CDS             6254..>7109
                     /locus_tag="AC16VR_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010298.1"
                     /translation="MLAASMVGCQSMPLDYAGLNDKIANGDQVAVGELRRAFLDLEDL
                     PERMERLRDLELQAAQLFEDEPLKLGSIGTAILDTYYGSLTGHFALSRFYEFVGSEEA
                     AVPHRQWQARIREDMEASADGSRESPYPAVTDVEAQMYAVSLGLSPVGSIYQTGEAFP
                     YTLLIQARPADGPLRNLYFDLGGWYESMRRQFPDENEFSPFALIGFQARRGDTAAQVA
                     VGAFLATQGRGDEAVDWLRAASRTGNLLANSLLARIFWEEANKDGEDDARQAALDEVL
                     ENYLHAVAL"
BASE COUNT         1303 a         2374 c         2234 g         1198 t
ORIGIN      
        1 tcgcgccgtc ggcggcggcg tagccaccga aatcgcgaaa gttcaccgcg cccgtgaggt
       61 cgacgaccct atccatgcaa ggttcccgtg gcagcgtgaa gaggcggcga gcttaccaga
      121 agcgcaagcc gagatggcag cctgccgccg ctcagcccgc ccgttcgtga ttcagcgcgc
      181 tcacctgctc gttgagcgtc cagtagtcgt agatcacgcc gatcccgaac aggcccacgg
      241 taaagagata gatgatcccg gtcatccact tgccctgata aaagcggtgg acgccgaaga
      301 aacccagaaa cgtgagcagc acccaggcca ccgtgtagct cttctcgccc tgctgatagc
      361 gccggtcggc ggacgcctcc aggttgggca tgaggatcag gtcgatgagc cagccgatga
      421 ggaacaggcc cagggtgaag aagtagatgg tcccgcttac gggccggccg aaatagaagc
      481 ggtgcgcgcc caggaacccg aatatccaga ggatgtatcc gaccgcaagg ctgtgcgtgt
      541 tgttcatgcg ggcctccagc agatcaggcg aaagatgctt cacgttgccc cgaaccggcg
      601 ccgcccgtca acggtgacgt gccgtgagct ccgcaatgcc ccggatgacc tcgggcccga
      661 ccggctccgg cagatcgtgc ccgatgccct cgagcaccag gagtctggac cgctcgagcg
      721 cctagtgggt atccacgccg ccctccacgg ggacgagggg gtcgtcgctg ccgtgaacga
      781 ggaaagccgc gacgtcgagc tgctccggcg ccggccaccg gttaccggcc gcggcgatgg
      841 cgatgccggc gatctggcgc gcaaaacctt cggggtagaa gctgcggttt aggtaagagg
      901 ggatcggcaa tttcagaacc agctgcgaga ttcgcaagct gcctcacaac atcggattgc
      961 acgccgggac gctctcggag tagggttttc tccacccgac aaggaacgtc ccgttgcagc
     1021 agctgcccga cccagtccgg atccccacca acgacatcga gctcgaggtt ttcgagcaag
     1081 gcgcgggcgc gccagtggtg ctgtgccacg ggtggccgga gcacgcctac tcctggcgac
     1141 accagatgag ccccctggct gaggccggct accacgtgat cgtgcccaac cagcgcggtt
     1201 acgggcactc gagccgaccg cagaaggtga ccgactacga catcacccat ctcgccgcag
     1261 acctcaccgg cctgctcgat catttcgggt acgaccgggc ggtattctgc ggccatgact
     1321 ggggcgccat cgcggtatgg aacctggcca tgctgcaccc ggacagggtg gccggggtga
     1381 tcaacttgag cgtacctctg ctgcgccggg ggcctacgga ctgggtctcg cactgggaaa
     1441 gcctcctcgg cgaagatttc tacatcgttc acttcaaccg ccggcccggc gtggcagacg
     1501 cggtcttcga ggaaaacccg cgacgcttcc tcggcaacct gtaccgtacc catcaatggc
     1561 aggagccagc agccgagccc cgatccggca tgccgctgat caacctcgca cgggcaaacc
     1621 ggccgcgcgg caggccgctg atgagcgacg ccgagctgga cgttttcgta cgtgccttcg
     1681 aacactccgg gttctccggc ggcatcaact ggtaccggaa cttcgcgcgc aactggaagc
     1741 tgaccgctga cgtgcccgag cacgttccgc acccggcgat gatgatctat ggccgctacg
     1801 acatcgtgcc cgtgttcgac aagctcaggg acttcgttcc gctggtggaa acccacgtgc
     1861 tcgactgcgg ccactggatc cagcaggaac tgcccggcga gaccaatcgc ctgatgctgg
     1921 actggctcga gcgaaactat ccttcgaacc gacgacggag cagccaatga acgccatcaa
     1981 gggcttgaac cacgccgcct atcgatgccg caactctgag gagacccggc agttttacga
     2041 ggatttcctg ggattgcccc tggtcgacgc cctgcccatc ggcactacca agaccggccg
     2101 cgccgcccag gtgctgcaca cgttcttcgc catggacgac ggctcctgcg tcgccttttt
     2161 cgaggagccg gacgcggcgt tcgacttcaa ggatcagcgc gacttcgatc tgcatctggc
     2221 actgaccgtc gacatggaga ccctccacga gatgttcgag cggggaaagg cgcagggcat
     2281 cgagacgcgc ggcatctcag atcacgggtt cattcactcg atctacttcc gcgatccgaa
     2341 cggctacgtg gtcgagctgg cggcacccgt ggagctgcga agcggcgcta gcgcggatga
     2401 gcggcggctg cgcgcccggc aggttctgga cgagtgggag gcacgacggc cctagctttc
     2461 ccgatgccgc ttgccgttca cgcggctcga gaccgctgca ggccggcgcc gcgtcaggac
     2521 gggatcatga agctcccgct ggcctccgcg accacctcct cggtgtccgt gcgaatcact
     2581 ttgccttcca tgatcgcgag gcggcccttg cgcttcaggc agcggccttc cagacgcacc
     2641 ggcgtgccca ccggcagctg cgcccggtag cggacgtccg cccgggcagt gaagcactgc
     2701 tcgccgcgca gccagatcca gttcgccatg acgtcgtcca gcaggctgaa gatgattcct
     2761 ccgtgtgcta ccttgtcgta gccgacgtgc tcttcgcccg gcgtgaattc cgccaggcag
     2821 acgtcctcct cgaggcgaaa gcgcacgtgg agaccgatgg ggtttccggg cccgcagacg
     2881 aagcagcggt tagcccgact ttctaacgac atgctgaatt tagagttgcc cgacccagcc
     2941 ggtatgatag cccaagtgca cgacctgaat ttaattcgat cccgcctgtc cgagcaccgc
     3001 gcggaagagt ttgcgccgga gcccgacatg cgggaggccg ccgtggccgt catcctgcgt
     3061 gaggtctcca gccggccggc cgagatcctg ttcatcaagc gggcggagaa gcccggagac
     3121 ccctggtcgg ggcacatggc ttttccaggc gggcaccggg acccggagga cgagagtctc
     3181 agagcggccg cagaacggga gactctggag gagatcgggc tggacctcga ttcggctcgc
     3241 tacctgggtc cgctgaacca gcagcgcgcc gtggcccgcg gccgccccct caacatgctc
     3301 atcgcgcccc acgttttcga gatcgccggg gacccttcgt tcaccatcaa ctacgaggtg
     3361 gcacaggtgg tctggacccc actggcaccg ctcgtatcag gggcgcttca cgataccgag
     3421 acccgtccgc tggcaggcac gccgacggtg ttcaacggct accgcctgcc ccagggacac
     3481 tttgtctggg gtctcaccta ccggatggtg aagtcgttct tcgccgccat cgacccgggc
     3541 tgggaacctc cgccggaggt ctagcgagcg ccgctgacgc ctaaacgtcc atgccgtgga
     3601 agttggcgat ggccaggcct gcggccacgc tggtgaacgg atcgtgctcc acgacccgcc
     3661 cgggaaatcg atcctccaga atgcgccgca ccgcgggaat caggctggag ccgccagtgc
     3721 acagcacgat gtccacgtcc ccatggacga gcccggcgag cgccaccgtt tcgtccagcg
     3781 cttgctccgc ctgttccagg aggtcgccga tcagcgattc gaaacccgca cgggtcaaac
     3841 gcacctccac gtcgatctcg gatatgtcca gcaccgcttc ttcccgagcc gaaagatctg
     3901 ctttgaagtc cttgatggtc tgaaacacca cgtagctcaa gttctgctgg atcagctccc
     3961 gaagccggcg gaacttgcgc gcgcccggac cgccctgccc tatgcactcc aagaccggcg
     4021 tggtgtagcg gttctggttg agcgtgtagg tcaccgccca gttgaccagg agctcctggt
     4081 aatcctcgaa ggggaagacg gtttcgatct cccggtccat accccgccgg cgccacgtct
     4141 cgcctttgcc gagcagcggg aagagcagct cgcgaaacag gcgctgatca atgtggtcgc
     4201 cgcccagcgc gataccatgg gtggtgacca cctcgaaatc gccggcagcc cgccgcagta
     4261 tgcagaaatc cagcgtgccg ccgccgaaat ccagggtgag cagatgctca ccggcagcct
     4321 cgggatgggc aaagagataa ctcacggcag cggcgatggg ctccggataa aagcgctgct
     4381 ccgtgatgcc cgcatacttg taagcctccc ccagtcgcgc cagagcagtc tggttgcgga
     4441 actcgtcccg gccttcgaag ttcaccggat ggcccaggtg agcgtagtcg gccccgctca
     4501 ccgtccggga gagagcctta cgcatgcgaa gcagtatcgg cgtgatgagg gcaaccaggc
     4561 ggaaaggatg atcgaaaacc atcaggcgcc tcacccgggg atcgcccagg agccgcttgg
     4621 tcccccggaa cagccgcccg tgcaggccgc tgtcgatcag cggctgcccg tagactttct
     4681 gggtgacggt ttccaccggc gcggggttct ccgaatcccc atgctccacg aaggcgctgg
     4741 tctctgcgat cacctctgga accagctcca cggttcggcc ggtattgtcg gcgatgtact
     4801 gattgatggc cgcctgaccg gtaagcgtct gcagctcacg gtcgatgtag gtggcggagg
     4861 gcatgatcgg atcggcgccc tccagcgcaa cgagctgcag ccgctcgccg tcgtaggtgg
     4921 ccgccgccga gttggtcgtg ccgaaatcaa tgccgacccc cacgcgcatc agttgccgcc
     4981 ctctcgttgc caattcatct ccaacgcctc gtcccggaaa ccttgccggc cggcgcaatc
     5041 acgcaagagt cacccggatg taagcacgcc ggcggaaccg ccccgtcaat cgcgagagga
     5101 gatcggcaag tcgcatctgc cacccgtacc ggaggcgcag cgccacgagc gccgcctggg
     5161 cgtccgcgcc ctcgatcagc tccgcgcgtg cctgcagcca ctccccctgc agcttgccct
     5221 tgacgtcaca ggcagcaacc cgcgcggtct ggtcacggcg caaacgtttg acctttccgg
     5281 catcacccgc cgagaagacg tagtaaacgc ccggcgcttc gcccgccgag ggagcaaacc
     5341 acaccggcgt ctcgaccgcc gagccgttct tcctgaaggt agccagattg aagtagcgtg
     5401 cgtcgtcgag cgaggtgccc accgacaatc tccgccgcga agcaagcggc ggagtttatc
     5461 gcgaattcaa accgccttca cgaggggcgc cacgttacag ccgactccgg tccctccaag
     5521 accgcaatag ccgttgggaa ccttcgcaag gtactgctga tggtacggct cggcatagta
     5581 gaacgccggg gcagacctta tctccgtcgt gatctcggaa aatccggcag cagaaagcgc
     5641 cttctgatat tcgtcggcgg acgcccgcgc ggcggccgcc tgctcgtcgt cgaacgtgta
     5701 gatcgccgag cggtactggg tgcccacgtc attgccctgg cgcatgccct gggtggggtc
     5761 gtgcgactcc cagaagacct tgagcagctc ggcgtaggaa accttctgag gatcgtatac
     5821 gacgagcacg acttcggtgt gccccgtcag cccggagcag acttcctcgt aggttgggtt
     5881 cggcgtgctg ccgccggcat agccgacggc cgtggagtac accccgggcg tgttccagaa
     5941 acaccgctcc gcgccccaga aacaaccaag agcgaacacg gcctgagcga ggccctcggg
     6001 aaacggttcc ttgagggggg cgccgttgac gtggtgtgcc gccggcacgg gcatctcctc
     6061 ggctcgtccc ggcagagcct cggattcact ggggatacgg agtttttgct cccaagccat
     6121 gtccaattgc ctgtaacatt gaaggaagat gaaaatcata catttcgccg ccccctccgc
     6181 aactgccagt tcgcccagcc gccgagccac tcgcctcttc cccttcggga ttcagggggc
     6241 ggctctgatc gtcgtgctgg cagcatcgat ggtcggctgc cagagcatgc ccctcgacta
     6301 cgccggcctc aatgacaaga ttgcaaacgg ggatcaggtc gccgtgggcg agctgcgcag
     6361 agcgtttctc gatctggagg atctgccgga acggatggag cgtcttcgcg atctggagct
     6421 gcaggcggcg cagctgttcg aggacgaacc tctgaagctg ggatccatcg gcaccgccat
     6481 tctggatacc tactacggca gcctgacggg tcacttcgcg ctttcgcggt tctacgagtt
     6541 cgtgggctcc gaagaggccg cggtacccca ccggcagtgg caggcgcgca ttcgcgagga
     6601 catggaggcg tccgcggatg gcagccggga atcgccttac ccggcggtta ccgacgtgga
     6661 agcccagatg tatgccgtca gcctgggctt gagcccggtg ggctccatct accagacggg
     6721 cgaggcgttc ccctacacgc tgctcatcca ggcgcggccg gccgatgggc ctctgcgcaa
     6781 cctgtatttc gatctgggcg gatggtacga gtccatgcgc cggcagttcc cagacgaaaa
     6841 cgaattttcg ccgttcgcgc tgatcggctt tcaggcccgg cgcggcgata ccgcagccca
     6901 ggtcgccgtc ggcgcgtttc tcgccaccca gggcagaggc gacgaagccg tggactggct
     6961 gcgagcggcc tcgcgtaccg gaaaccttct cgccaactcc cttctcgccc gaatcttctg
     7021 ggaggaggcg aacaaggacg gcgaagacga tgcccggcag gccgcgctgg acgaggtgct
     7081 ggaaaactac ctgcacgccg tcgcgctcg
//
LOCUS       DBNHQR010000164         4703 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1599173, whole genome shotgun sequence.
ACCESSION   DBNHQR010000164 DBNHQR010000000
VERSION     DBNHQR010000164.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4703)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1599173"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="AC16VR_03735"
     CDS             complement(<1..489)
                     /locus_tag="AC16VR_03735"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6010299.1"
                     /translation="MIARAPGRHALLFILITIFADTVAFGIIIPVLPKLIAELGDTSI
                     SEAASYGGLLMFVFAVMQFLFAPVIGNLSDRFGRRPVLLISLAALGIDYVIMGLAPSL
                     VWLFLGRMVAGVAAATFATANAYVADITPDEQRAARFGLIGAAWGVGFVLGPAIGGLL
                     GEM"
     gene            complement(544..774)
                     /locus_tag="AC16VR_03740"
     CDS             complement(544..774)
                     /locus_tag="AC16VR_03740"
                     /inference="COORDINATES: protein motif:HMM:NF044586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010300.1"
                     /translation="MSKGTTSQSSNADLRRQLDADVEKFLKSGKKIQQIPTGVSSQDY
                     TKGRKHIVLGKSKPRTPETRTPETRTPEKGSE"
     gene            complement(883..1290)
                     /locus_tag="AC16VR_03745"
     CDS             complement(883..1290)
                     /locus_tag="AC16VR_03745"
                     /inference="COORDINATES: protein motif:HMM:NF013722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR6010301.1"
                     /translation="MQVEAGDVIPEWVMERVAPERMRTMAAILRDPNPVHWDRKAVAA
                     LGFGDHTINQGPLGLSYMINMLHEWAGPHCLKRVFMRFPQVVLDEDHVIARGVVTAVR
                     EVGGAREADCEIWLEREGAGRLLEGSAVVRLPD"
     gene            complement(1280..1729)
                     /locus_tag="AC16VR_03750"
     CDS             complement(1280..1729)
                     /locus_tag="AC16VR_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010302.1"
                     /translation="MTMPDASEIEALVGHEFPGGKYTVAHWENFLLTECTGAEQLPEG
                     MVHPVVMFHMPILGAGTSIGEMFALGQAESDYSIGIESYDWEFFGTLEEDVEYSISAR
                     VTEADRRRSGDRVYDRIQFQFDVADPGGTVLARSTITWTYGRSGDAS"
     gene            1851..2402
                     /locus_tag="AC16VR_03755"
     CDS             1851..2402
                     /locus_tag="AC16VR_03755"
                     /inference="COORDINATES: protein motif:HMM:NF024092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6010303.1"
                     /translation="MSKIDPYASWLPLEARAAAETNPRHKQLLLEVRDHMEHEIKGAL
                     EPLMATLTAEPVYHFWGNEPSVLNGRDAVRDFYKGMIAMGGQQFQVVVDRIVVDDGAV
                     VTEGQVKQVYKGAALKAMGMDAVGGERVADDDLFMTTAQLVTVWPADAVGKLIGEDIY
                     FGHDPFRNVERIGREDLPDYYQI"
     gene            2402..4111
                     /gene="ggt"
                     /locus_tag="AC16VR_03760"
     CDS             2402..4111
                     /gene="ggt"
                     /locus_tag="AC16VR_03760"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione gamma-glutamate
                     hydrolase [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIR6010304.1"
                     /translation="MIPKTKTAYYGIGWLLMLLALTGQTALAASAAHRAQHGMVVTQS
                     AIASDVGARVLRAGGNAIDAAVATAFALAVTYPEAGNIGGGGFLVYQPQEGGADAYDF
                     RETAPTGSHPQMWLDEDGEYSPARQYVGPVAAGVPGTVAGLHKAWRDHGSLPWAELVA
                     PAIGLARDGFAIPEDLARSIAATLPQMEAYPASLAQFSRDGAPMAPGELLVQRDLART
                     LELIAADGPDAFYRGPIAEQIVALMASTGGLITAGDLASYRAVKRKPIRGTYRGYEIV
                     SMPPPSSGGVALVQTLNLLEGYDIAGEGFGSARTLHLITEALRRTFADRARYLADPDF
                     VKDMPVRRLTSKSYAAELRETISLNRAGTSSPETFEWPTASNDTAHFSVVDAQRNAVS
                     LTYTLNGRYGNQMVVPGAGFLLNNEMGDFNPVPGLTDERGRIGTTPNLAAPGKRMLSS
                     MSPTIVLKDGELFMVTGARGGRSIISTVLQTIMNVVDHGMNAQAAVDARRIHHQWLPD
                     RLRYEPQSFSRDTLNALRWLGHEIEQGGASWSNAEVIVVANDGWLEGGDDRRRSRGSV
                     AGY"
     gene            4153..>4703
                     /locus_tag="AC16VR_03765"
     CDS             4153..>4703
                     /locus_tag="AC16VR_03765"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6010305.1"
                     /translation="MKIGLSNFPTEYSISAVALARAAEERGFESLWVVEHTHIPASRR
                     TPYPIGGDLPSIYWESYEPFTFLAQAAAVTTDLRVGTGICLVPEHHPIALAKRVASLD
                     ALSGGRFLFGVGAGWNAEELENHGVAFGDRWKVLREHVLAMKACWTEKDAEYHGRFVD
                     FDPVWVEPKPVQKPHPPIYIGASS"
BASE COUNT          861 a         1592 c         1518 g          732 t
ORIGIN      
        1 catctcgccg agcagcccgc caatggccgg gccgagcacg aaccccacac cccaggccgc
       61 tccgatcagg ccgaagcggg cagcccgctg ctcgtcgggc gtgatgtcgg ccacgtaggc
      121 gttggcggtg gcgaaagtcg ccgccgccac gcccgcgacc atgcggccga gaaacagcca
      181 cacgaggctg ggcgccagcc ccatgatcac gtagtcgatg ccgagcgcgg ccagggagat
      241 cagcagaacg ggccggcgac cgaagcggtc cgaaaggttt ccgatcaccg gcgcgaagag
      301 aaactgcatg accgcgaaga cgaacatcag aagaccgccg tagctggccg cttcgctgat
      361 ggaggtatcc ccgagttcgg cgatcagctt cggcaacacc gggatgatga tgccgaacgc
      421 aaccgtgtcc gcgaagatgg tgatgaggat gaacagcagc gcgtgccgtc ccggcgcgcg
      481 cgcgatcacg cgatcatgtc ccgggctggc acgccgaccc cggcgggaac atcagggtga
      541 cggctactcg gaccctttct cgggagtccg cgtctcggga gtccgcgtct cgggagtccg
      601 cggtttcgat ttgccgagaa cgatgtgctt gcggcccttg gtgtagtcct gcgagctgac
      661 cccggtcgga atctgctgaa tcttcttgcc cgactttaga aatttctcga cgtccgcatc
      721 gagttgccgg cgaaggtccg cattcgacga ctgactggta gtgcctttgc tcatgatgaa
      781 gagggtccgc taacgttcgg cgacgacggt attcgccgtc actggaagat gcatcatgcc
      841 cgcacggagc gagcaaagcc agcggacctc cccggaaagc ggctaatccg gcaaccggac
      901 gacggccgag ccttccagga gccggcccgc tccctcgcgc tccagccaga tttcgcaatc
      961 cgcctcgcga gctccaccca cctccctcac ggcagtgacg acgccgcggg cgatcacgtg
     1021 atcctcgtcg agcaccacct ggggaaaacg catgaacacc cgcttgagac agtgcggacc
     1081 ggcccactcg tgcagcatgt tgatcatgta gctcaggccc agtggaccct ggttgatggt
     1141 gtgatcaccg aagcccagcg cggcaaccgc tttgcggtcc cagtgcacgg ggttgggatc
     1201 gcggagaatg gcggccatgg tgcgcatgcg ctccggcgcc acgcgttcca tgacccactc
     1261 cggaatgacg tcgcctgcct caacttgcat cgccgctcct cccatacgtc caggtgatgg
     1321 tgctccgggc gaggacggtg ccgcccggat cggccacgtc gaactgaaac tggatccggt
     1381 cgtagacgcg atccccactg cggcgacgat cagcctcggt cacgcgtgcg gagatcgagt
     1441 actccacgtc ctcctccagc gtgccgaaga actcccagtc atagctctcg atgccgatgg
     1501 agtaatcgga ttccgcctga cccagggcga acatctcgcc gatgctcgtg ccggctccga
     1561 gaatcggcat gtggaacatg accaccgggt gcaccatgcc ctcgggcagc tgctctgcgc
     1621 ccgtgcactc ggtcagcagg aagttctccc agtgagcgac cgtgtatttg ccgcccggaa
     1681 actcgtggcc cactagagcc tcgatttcgg acgcgtccgg catcgtcatg ccacattccc
     1741 cttcttgatc cagcgaccaa cattcaaacc aatgaccggg acctggtcaa cggacggttc
     1801 agcctgacgc gtcgggccgg taacatctgc agaccgcaaa ggagacccag atgtcaaaga
     1861 tcgacccgta cgccagctgg cttcctctgg aagcgcgtgc agcggccgaa accaatcccc
     1921 ggcacaagca gctgctgctg gaggtacgcg accacatgga gcacgagatc aaaggcgcgc
     1981 tggagccact aatggcaacg ctgaccgcgg agcccgtcta ccatttctgg ggcaacgagc
     2041 cgtcggtgct caacggccgc gacgcggtca gggatttcta caagggcatg atcgccatgg
     2101 ggggtcagca gttccaggta gtcgtcgacc gcatcgtggt ggacgacggc gccgtggtta
     2161 ccgaagggca ggtaaagcag gtctacaaag gcgcagccct gaaggccatg ggaatggacg
     2221 ccgtgggcgg tgaacgggtg gcggacgacg atctgttcat gaccaccgcc cagctggtca
     2281 ccgtctggcc cgccgacgcc gtcggcaagc tcatcggcga ggacatctac ttcgggcacg
     2341 atccgttccg caacgtcgag cggatcggcc gggaagatct gcccgactac taccagatct
     2401 gatgatcccc aagaccaaga ccgcatacta cgggatcggc tggctgctaa tgctgctggc
     2461 ccttacgggc cagacggccc tggcggccag cgccgcgcac cgcgcccagc acggcatggt
     2521 ggtcacccag agcgccatcg cctcggacgt cggcgcccgg gtgctgcggg cgggcggcaa
     2581 cgccatcgat gctgccgtgg cgaccgcatt tgcgctggca gtgacctatc cggaagccgg
     2641 caacatcggc ggcggcggtt tcctggtgta ccaaccccaa gagggcggcg cggacgccta
     2701 cgactttcgg gagaccgcgc ccaccggatc acacccgcag atgtggctgg acgaagacgg
     2761 cgagtactcg ccggcgcggc agtacgtcgg ccccgtggcc gccggcgtgc cgggaacggt
     2821 cgccggcctg cacaaggcat ggcgcgatca cgggtccctg ccctgggcag agctggtggc
     2881 gcccgccatc gggctcgccc gcgacggttt cgccatcccc gaggacctgg cccgcagcat
     2941 agcggccacc ctgccgcaga tggaggccta cccagcctcc ctggctcagt tcagccgaga
     3001 cggcgcgccc atggcgcccg gtgagctcct ggtgcagcgc gatctggccc gaaccctgga
     3061 actcatcgcc gccgatggtc ccgacgcctt ctaccggggc ccgatcgccg agcagatcgt
     3121 cgcgttgatg gcctccaccg ggggcctgat caccgccggg gatctggcgt cctaccgagc
     3181 cgtcaagcgc aagccgatcc gcggcacgta ccggggctac gagatcgtct ccatgccgcc
     3241 gcccagctcg ggcggcgtgg cgctggtgca gacgctcaac ctgctggagg gctacgacat
     3301 cgccggggag ggcttcggtt ccgcccgcac tctgcacctg atcaccgagg cgttgcgccg
     3361 cacgttcgcc gatcgcgcgc ggtatctggc cgacccggac ttcgtcaagg acatgcccgt
     3421 gcggcgcctg acatcgaagt cctacgccgc cgagctgcgc gaaacgatct cgctgaaccg
     3481 cgcaggcacg tcctcaccgg agaccttcga gtggccgacg gccagcaacg atacggccca
     3541 tttctcagtc gtggacgccc agcgcaacgc cgtgtcgctg acctacacgc tcaacggccg
     3601 gtacggcaac cagatggtgg tgccaggcgc cggctttctg ctgaacaacg agatgggcga
     3661 tttcaatccg gtgcccgggc tgaccgacga gcgcgggcgc atcggcacca cacccaacct
     3721 cgcggcaccc ggcaagcgca tgctctcgag catgtcgccg acgatcgtgt tgaaggacgg
     3781 cgagctcttc atggtgacag gagcgcgggg cggacgctcc atcatcagca ccgtgctgca
     3841 gaccatcatg aacgtggtcg accacggcat gaacgcccag gcggcggtgg acgcccgtcg
     3901 aattcatcat cagtggctgc cggaccgact gcgctacgag ccgcagagct tttcgcgcga
     3961 cacgctgaac gcgctgcgct ggctgggcca cgagatagag cagggcgggg catcctggag
     4021 caacgccgag gtcatagtgg tagcgaacga cggctggctg gagggcggcg acgaccgccg
     4081 acgctcccgc gggtccgtgg ccgggtactg agcgcacgcg gcgaccgttt caactcaatg
     4141 aggagcaacg acatgaagat cgggctgtcc aactttccca ccgaatattc catctccgcc
     4201 gtggcccttg cccgagcggc cgaggaacgc ggcttcgagt ccttgtgggt ggtggagcac
     4261 acccatatac ccgcctcccg gcggacgccc tacccaatag gcggcgacct gccgagcatc
     4321 tactgggaaa gctacgagcc gttcacgttc ctcgctcagg cagccgccgt gaccaccgac
     4381 ctgcgggtcg gcaccggtat ctgcctggtc ccggagcatc accccatcgc cctggcaaaa
     4441 cgggtggcgt ccctggacgc cctgtcgggc ggacgcttcc tgttcggcgt gggcgcggga
     4501 tggaacgccg aggagctgga gaaccacggc gtggcgttcg gcgaccgctg gaaggtgctg
     4561 cgagagcacg tcctggccat gaaggcatgc tggaccgaga aagatgccga gtaccacggc
     4621 aggttcgtcg acttcgatcc cgtctgggtg gaacccaagc cagtgcagaa gccccatccg
     4681 ccgatataca tcggggcgtc ctc
//
LOCUS       DBNHQR010000165         2158 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1409023, whole genome shotgun sequence.
ACCESSION   DBNHQR010000165 DBNHQR010000000
VERSION     DBNHQR010000165.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1409023"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="AC16VR_03770"
     CDS             complement(<1..303)
                     /locus_tag="AC16VR_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HIR6010306.1"
                     /translation="MSEAVLKIFSYLPNPRVWKAQIAAELCGVAVDVVGDRPSALQNW
                     LWDSEARPLEDAERTADSPHARESRRGFSGLLFKTDAFLTAHPFGTVPAAFAPGGEP"
     gene            411..2120
                     /locus_tag="AC16VR_03775"
     CDS             411..2120
                     /locus_tag="AC16VR_03775"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl-D-amino-acid deacylase family protein"
                     /protein_id="HIR6010307.1"
                     /translation="MHDLIIRNAAIVDGTGAPRREGDVVVDDGTIVEVAAKGSAPASA
                     ARVIDAGGDLLTPGFVDIHTHYDGQVCWDKQITPSCWHGVTSIVMGNCGVGFAPVRPG
                     AEDELVALMESVEDIPGTALHDGIPWGWETFPEYLDAIDTPYVMDVGAQVPHVAIRHY
                     VMGERCYDDATPEDMTAMRDLTRTALEAGALGFSTSRFYGHVDKAGRLVPGTRASADE
                     MRAIAGAFEGLGYGTMEFVSDQLDDPAELAWIERITRATGCTVTPLTTAGFGPLWTLA
                     EKLDAEGFNLRPQVGARPASILMTLDGTINPRRQFPAYREIHERPLAERRAMLKDPAF
                     RQRVLTEEPILPRNADAVRFITDHEHMYVMDAALSYEPGPEDTVAAVAGSRGVDPREV
                     MMDTLADGIPLLVLFGRYDGDLEGQRLAIEHPLSVFGLSDGGAHCGVLVDASVPTYML
                     SYFTRDRSRGPLMPLEFVVHKLTRDSAGVYGLHDRGVIAPGYRADFNLIDYDALGLEP
                     PEMAYDLPAGGKRLVQKSRGYRMTIQSGQVTFEQGEATGATPGRLIRGARPAPVPAPL
                     RAR"
BASE COUNT          334 a          759 c          747 g          318 t
ORIGIN      
        1 gggctcgcct cccggcgcga acgcggcggg gacggtgccg aagggatggg cggtgaggaa
       61 ggcgtcggtc ttgaagagaa ggccggagaa accccgtcgg ctttctctgg catgggggct
      121 gtccgccgtg cgctcagcgt cctccagcgg ccttgcttcg gagtcccaca gccagttctg
      181 cagagcactg ggccggtcgc ccaccacgtc cacggccacg ccgcagagct cggcggcgat
      241 ctgggccttc cacacccggg gattgggcag gtaggagaag atcttcagga cggcttcgga
      301 catcgtgggc tcctcgtcag ggtctgggtg ccgatcgcgc cggcctgacc ggacctcatg
      361 gtagtatccg aggcaatcag ggatccagcg aagggtaggg gagacagccc atgcacgacc
      421 tcatcatccg caatgccgcc atcgtcgacg gcaccggcgc accccgcaga gagggcgacg
      481 tggtggtgga cgacggcacc atcgtcgagg tggccgcgaa ggggtctgcg cccgcgagcg
      541 ccgcccgggt catcgacgcg ggcggggatc tcctgactcc cggcttcgtg gacattcaca
      601 cccactacga cggccaggtg tgctgggaca agcagatcac gccgagctgc tggcacgggg
      661 tgacctccat cgtcatgggc aactgcggcg tgggctttgc cccggtgcgt cccggcgcgg
      721 aagacgagct ggtggccctc atggagagcg tggaggacat ccccggcacg gcgctgcacg
      781 acggcatacc ctggggctgg gagacgtttc ccgagtatct ggacgccatc gacaccccct
      841 acgtcatgga cgtgggcgcc caggtgcctc acgtcgccat ccgccactac gtcatgggcg
      901 agcgctgcta cgacgacgcc acgcccgagg acatgacggc catgcgcgac ctcacacgga
      961 ccgccctgga ggcgggcgcc ctgggattct ccacctcccg cttctacggc cacgtggaca
     1021 aggccggccg gctggtaccc ggcacccggg cgtccgccga cgagatgcgc gccatcgccg
     1081 gcgccttcga gggtctgggt tacggaacca tggagttcgt ctccgaccag ctggacgacc
     1141 cggcagaact ggcctggatc gagcgcatca cccgcgcgac gggctgcacg gtgacgccgc
     1201 tgaccaccgc gggcttcggc ccgctctgga ccctcgccga gaagctcgat gcggaagggt
     1261 tcaacttgag gccccaggtg ggcgcccggc cggcctccat cctcatgacc ctggacggca
     1321 ccatcaaccc caggcggcag tttcccgcct atcgcgagat tcacgaacgg cctctggccg
     1381 agcgcagggc catgctgaag gacccggcct tccgccagcg ggtcctgacc gaggaaccca
     1441 tcctgccccg caacgccgac gccgtgcgct tcatcaccga tcacgagcac atgtacgtga
     1501 tggacgccgc gctcagctac gagccgggcc cggaggatac ggtggcggcg gtggccggct
     1561 cccgcggcgt cgacccgcgg gaggtgatga tggacaccct ggccgacggc attcccctgc
     1621 tcgtgctgtt cggccgctac gacggtgatc tggaaggcca gcggctggcc atcgagcacc
     1681 ccttgagcgt gttcgggctc tccgacggtg gcgcccactg cggcgtgctg gtggatgcca
     1741 gcgtgcccac ctacatgctg agctacttca cccgggatcg cagccgcggg cccctcatgc
     1801 ccctggagtt cgtggtgcac aagctgaccc gggactccgc cggcgtttac ggcctgcacg
     1861 atcgcggcgt gatcgctccc ggttaccggg ccgacttcaa cctcatcgac tacgacgccc
     1921 tgggcctgga gccgccggag atggcctacg acctgcctgc cggcggcaaa cggctggtgc
     1981 agaagtcgcg cggttaccga atgaccattc agtccggcca ggttacgttc gagcagggcg
     2041 aggccaccgg agcgacgccc gggcggctga tccggggcgc gcggccggcg cccgtgccgg
     2101 cccccctgcg ggcgcgctga tcccagcggc gggcgcggcc ccgatggcgc atccctgg
//
LOCUS       DBNHQR010000166         8875 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1472626, whole genome shotgun sequence.
ACCESSION   DBNHQR010000166 DBNHQR010000000
VERSION     DBNHQR010000166.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8875)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8875)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8875
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1472626"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3308)
                     /locus_tag="AC16VR_03780"
     CDS             complement(<1..3308)
                     /locus_tag="AC16VR_03780"
                     /inference="COORDINATES: protein motif:HMM:NF053188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HIR6010308.1"
                     /translation="MATSRENFALDWVKGALQESLDEAIAALDSFAEAGHGEAQMREC
                     MTALHQVHGALVMLELKGTTLLAEHLERLAQHLMIGAVGDSVGAGQCLMQGLLELPGY
                     LDEVQRGLSDAIEPMIPLINEVRGHLDERPLEGAMVTDLGVASEAAIERFDGIDGVEK
                     AGRIRAAYQQVLLCILRGDDRERAVITLRKIAEVLSRVCLGTPHETQWRALGEFAASL
                     ATDTGPIDPDAAKLLRRIDSEIRTLSRDGLGVAGSPVSLDLIKQLLDACRQRGYRSEA
                     ITDISNALAGRGDGGGLAMNGLKALVSASLVVREELALVKDQLDLFARARGRASVDLN
                     SLLAPLKQIRSTLTLLGFASAHEIVTQQLEALEQLASAEAVDPQDLFSVASALVRVDE
                     SMASFTEGDQEVEKITNDAQRTVTVEVRNGLEPVRQAVLEYIAAGWDSEPLAGVPEQI
                     AALCGALEMIPLRRAARILSSCGAYLRRDLLEAGATPGMDAMETLADAITAVDYYLQR
                     TAEDITGGIEDILDVADASLEKLGYAGIVDERPAAQPGAAFQAETAGVEETAEEGFDW
                     EGFVSEAADLREEPASSDAQDQEAPESEPELIDFEPVALEGGSDDEPEAAQAASADAE
                     PEAEPEDASAVEETPVAASAPSPALPPLSPAIAEKTSRLADAFESDEEIVEIFVEEVD
                     EVLETINGCLPRWGEHLSDEDSLTELRRAFHTLKGSGRMVGANIIGELAWSVENMLNR
                     LIDGTFEPTGQFALVVEEATSLMPYLRDAFEAQRAPDLDAMGRIMEKADVLASGGSLE
                     EQAPVAEEEPAVEAAEAGEGFEALGLFVSESEEHLAVLALCDDGEDFVLDEDAYRALH
                     TLAGSAGMAGIEVVSELARPTYEVALAARSEQEVTRASGAVAEFLRAATTALACMIET
                     LRAGSEPEEELQLIADADRLLVEIQETGESKLDELMKLPALAVVMDAEEFLGGWARGV
                     MDLEFAGKLIGALGEIADLARATERSAVADLAAALRATHEELEQETISPESHVALSRA
                     HETLLQQFDAMAAQQTVALATAEIASLTSLREAVTEPSSVEAFGTGAGSSAGSDYAEG
                     AGSRGAEDVAAGDTG"
     gene            complement(3318..4421)
                     /locus_tag="AC16VR_03785"
                     /pseudo
     CDS             complement(3318..4421)
                     /locus_tag="AC16VR_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014851231.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(4566..5108)
                     /locus_tag="AC16VR_03790"
     CDS             complement(4566..5108)
                     /locus_tag="AC16VR_03790"
                     /inference="COORDINATES: protein motif:HMM:NF013731.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HIR6010309.1"
                     /translation="MNGQAQNILTDIRATTIANAAPLPSRENEHLEWQGVGYQLGGVR
                     LVSPMGEISEILAVPKFATLPGVKPWMLGVANVRGRLIPIIDLHNFLDMRTTTPMVQW
                     RVLVVEDEELIAGFVVEQSLGIQHFLEDSFEESTAEEVAALKPYLRGAFHQGGCVFYE
                     AHLKSILRDEKFFDVADKTN"
     gene            complement(5117..5482)
                     /locus_tag="AC16VR_03795"
     CDS             complement(5117..5482)
                     /locus_tag="AC16VR_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010795388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6010310.1"
                     /translation="MARILVVDDSPSETHQLANALSRHGHQVLTAASGSDGISLANEE
                     QPDIILMDVVMPGINGFQATRQLTRSQGTSHIPVIIVSSKSQDADRVWGKRQGAQGYL
                     TKPVDDRTLISTVNEALSA"
     gene            complement(5508..5888)
                     /gene="pilG"
                     /locus_tag="AC16VR_03800"
     CDS             complement(5508..5888)
                     /gene="pilG"
                     /locus_tag="AC16VR_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015475142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility response regulator PilG"
                     /protein_id="HIR6010311.1"
                     /translation="MEQELKGVKVLVIDDSKTIRRSAENLLTKEGYDVITATDGFDAL
                     AKIVDSRPGIIFVDVMMPRLDGYQTCALIKNNSEFKTTPVVMLSSKDGLFDKAKGRIV
                     GSDQYITKPFSKDELLGTIRNLVH"
     gene            6144..7130
                     /gene="gshB"
                     /locus_tag="AC16VR_03805"
     CDS             6144..7130
                     /gene="gshB"
                     /locus_tag="AC16VR_03805"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004419666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HIR6010312.1"
                     /translation="MSLKIAFVMDPLPTLSLKKDSTLAMIRAAQLKGWQVGYLEQGDL
                     LLHEGAPCGMLRSLELVGPFADSLDPAEAGDRWYQLSEAALTPLAELDVIMMRKDPPF
                     DMEYIYSTYLLERAEALGVLVVNKPQGLRDCNEKFFATAFQECCPPLVVTRSEAALRE
                     FQRTHGNVVFKPLDGMGGQGVFRIMEGDENLGVIIETLTKGGTAQIMGQKFIPEIASG
                     DKRVLLIDGEPIPYALARIPMAGEARGNLAAGGTGEGRELTERDRWIAAQVGPELRRR
                     GLLFVGMDVIGDYLTEVNVTCPTCIRELDAQFSLNIGGMLMDTISAKLDAQA"
     gene            7155..8015
                     /locus_tag="AC16VR_03810"
     CDS             7155..8015
                     /locus_tag="AC16VR_03810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR6010313.1"
                     /translation="MSAAAITPRDRLGFTLFLAVSLHAAIILGVSFTADLDLLESPTV
                     EVTFAQHSDLEAPEDADFIAQTNQLGSGTESEVLETTTDQEAEFFANEFADAISNPLP
                     TIEQNQLDSRELVTTISEVDDVAPIEEETPVEENVNPQPATDIRFDDLAKEIASLEAR
                     IAADQQAKAKQPRVKRLTSVSAKSADEAAYLNMWRQKVERIGNANYPDSRVYGNLRML
                     VVLSYDGTLKDVRILESSGHKSLDDAALRIVRLAAPYQQFPVEMRKKYDQLEIIRTWK
                     FSRSGARLGS"
     gene            8005..8565
                     /locus_tag="AC16VR_03815"
     CDS             8005..8565
                     /locus_tag="AC16VR_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003297209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HIR6010314.1"
                     /translation="MAADTSLKNQFLLAMPGLAGTYFGDTVTYVCEHSADGAMGLMVN
                     RPTEVTLQALLADLGIDSSAVSADIPVMEGGPVASERGFVLHSDDRAFDSSLSLGDGL
                     MLNSTREVLEAIAAGEGPADFLVAMGYAGWGGGQLEQELLENAWLTCPGNTDVLFRTP
                     FAERVNQAAAALGIDFRLMSSQAGHA"
     gene            8573..>8875
                     /gene="ruvX"
                     /locus_tag="AC16VR_03820"
     CDS             8573..>8875
                     /gene="ruvX"
                     /locus_tag="AC16VR_03820"
                     /inference="COORDINATES: protein motif:HMM:NF015607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HIR6010315.1"
                     /translation="MKQGHVLAFDFGLKHIGVAVGQSVTRTASPLITIAARDGTPDWS
                     DVERLISEWQPAVLLVGLPLNMDDSESEMSLKARGFAERMGRRFGVPIELVDERLTS"
BASE COUNT         1561 a         2902 c         2718 g         1694 t
ORIGIN      
        1 ccggtgtcgc ccgcggcgac atcttctgcg ccgcgggatc ctgcaccctc ggcgtagtcg
       61 ctgccggcag aagagcccgc tccggttccg aacgcttcca ccgaggacgg ttccgtgacc
      121 gcctcacgca gtgacgtgag cgatgcgatc tccgcggtag ccagggcaac cgtctgctgg
      181 gcggccatgg cgtcgaactg ttggagcagg gtctcgtggg cgcgcgatag agccacatgg
      241 ctctccgggc tgatcgtctc ctgctcgagc tcctcgtgag tggctcgcag cgcggctgcc
      301 agatcggcga ccgctgagcg ctcagtcgct cgcgcgaggt cagcgatctc gcccagagcc
      361 ccgatcagct taccggcgaa ctcgaggtcc atgacgccgc gagcccaacc gcccagaaac
      421 tcctctgcat ccatgacgac tgccagggcc ggcagcttca tgagctcgtc gagcttgctc
      481 tctccggtct cctgtatctc caccagcagc cgatcggcat ctgcgatgag ctgcagctct
      541 tcctcgggct ccgagccggc tctcaacgtc tctatcatgc aggccagcgc cgtcgtcgcg
      601 gcccgaagga actcggcaac cgcacctgaa gctcgcgtga cctcctgttc agaccgggcg
      661 gcgagagcca cctcataggt aggacgggcc agctcgctca ccacctcaat gcccgccatg
      721 cccgcactgc cagccagggt gtgcagcgct ctgtaggcat cctcgtcgag aacgaaatct
      781 tctccgtcgt cgcagagggc aagcacggcc agatgctcct cggattccga tacgaacagg
      841 ccaagcgcct cgaagccctc gcctgcttcc gcagcttcca cggccggctc ttcctccgcg
      901 accggcgcct gctcctccag ggagccgccc gacgccagca cgtcggcctt ctccatgatt
      961 cggcccatag cgtcgagatc gggggcccgc tgggcttcga acgcatcgcg cagatacggc
     1021 atcaacgacg ttgcttcttc aacgaccagc gcaaactgtc cggtgggctc gaaggtgccg
     1081 tcaatgagcc ggttgagcat gttctccacc gaccaggcca gctcgccgat gatgttggcg
     1141 ccgaccatgc ggccgctgcc cttgagcgtg tgaaacgcgc gacgcagctc ggtaagcgag
     1201 tcctcgtcgc tcagatgctc gccccagcga ggcagacacc cgttgatggt ctccagaact
     1261 tcgtcgactt cctccacgaa tatctcgacg atttcctcgt cggattcgaa cgcgtcggcc
     1321 agccggctgg tcttttcggc gatggccggc gacagcggcg gcagcgcggg tgacggcgcg
     1381 ctggcggcca ccggagtctc ttccacagcg gaggcgtcct ccggctctgc ttccggctcg
     1441 gcgtcggcgg aagcagcctg cgctgcttcc ggctcgtcgt ccgagccgcc ctcgagggca
     1501 acgggctcga agtcgatgag ctccggctcg gattcgggcg cttcctgatc ctgagcatcc
     1561 gacgaggcag gctcttcgcg caggtcggca gcctctgaga caaagccctc ccagtcgaag
     1621 ccttcctccg cggtctcttc gacgccggcg gtctcggcct gaaacgctgc gcccggctgc
     1681 gcggccgggc gctcgtcgac gatgcccgca tacccgagct tctcgaggct tgcgtccgcg
     1741 acgtctagaa tgtcctcgat cccgccagta atgtcctcag ccgtgcgctg gaggtagtag
     1801 tcgacggccg tgatggcgtc tgccagggtt tccatggcat ccatgcccgg ggtggcaccc
     1861 gcctccagca ggtctcgtcg caggtatgcg ccacagctgc tcagaatccg ggccgcgcgc
     1921 cgcagcggaa tcatctccag agcgccgcac aaagcggcaa tctgctccgg cacgccggcg
     1981 aggggttccg agtcccaacc cgcggcgatg tactcgagca cggcctgtct gaccggctcg
     2041 aggccgttac gcacctccac ggtgacggtg cgctgggcgt cgttggtgat cttttcgacc
     2101 tcctgatcgc cctcggtgaa gctcgccatg ctctcgtcta cacgtacgag cgcgctggcg
     2161 acgctgaaga ggtcctgggg gtcgacggct tcggccgacg ccagctgctc gagcgcttcg
     2221 agctgctgcg tgacgatctc gtgagcactt gcgaaaccga gcagcgtcag cgtgctgcgg
     2281 atctgcttga ggggcgccaa gagcgagttc aggtcaacgc tggcacgacc acgcgcgcgc
     2341 gcgaacaggt ccagctggtc tttgaccagc gcgagctctt cgcgcaccac caagcttgcg
     2401 ctcaccaggg cttttaagcc gttcatggcc agcccgccgc cgtctccgcg gcccgccagc
     2461 gcattgctaa tgtcggtgat cgcttcgctg cgataccccc gctggcgaca cgcatccaga
     2521 agctgcttga tcaggtctag agaaaccggg ctgccggcaa ctccgagacc gtctctggac
     2581 agcgtgcgga tctccgagtc gatccggcgc agcagctttg ccgcgtccgg gtcgatgggg
     2641 ccggtgtccg tggccaggct cgcggcaaat tcgccgagcg cgcgccactg ggtctcatga
     2701 ggagtgccca ggcataccct ggacagcacc tcggcaatct ttcgcagggt gatgaccgcc
     2761 cgttcgcgat cgtctccccg caggatgcag agcaggacct gctggtaagc cgcccgtatc
     2821 cgccccgcct tctctacgcc gtcgatccca tcgaagcgct ctatggccgc ttcgctggcg
     2881 acgcccaggt cggtgaccat cgcgccttcc aacggccgct cgtccagatg accgcggacc
     2941 tcgttgatca gcgggatcat gggttcgatc gcgtcggaga gcccgcgctg cacctcgtcg
     3001 agatacccgg gcagctcgag aagcccctgc atgaggcact gaccggcccc aacgctatcg
     3061 ccgaccgcac cgatcatcag gtgctgggcc agccgctcca ggtgctcggc cagcagcgtg
     3121 gtgcccttca gctccagcat caccagagcg ccgtgcacct ggtgcagagc cgtcatgcat
     3181 tcgcgcatct gcgcttcgcc gtgaccggct tcagcgaacg agtctagcgc cgcgatcgct
     3241 tcgtccagag actcctgcag cgcgcccttt acccagtcga gggcaaaatt ttcccggctt
     3301 gtcgccatac cgaggtctta atcctggctt tggtactggg ccggcagctt gaagccttcg
     3361 accgattcgc gcatctcgtt ggccatggcg gccaggttac cgatcgaagt cgccgtttgc
     3421 tcggtgctgt caacggtctg cgcggtaatc tcttgaatga cgttcattgt attggaaacg
     3481 tggcccgcag aggcagcctg ctggcgtgcc gcgttgctga tgttctggat cagctcggcc
     3541 agcgacgtgg atacgctttc gatctcctcg agagccaccc cggcgtcctg ggcgcgcttg
     3601 gcaccgttga cgacctctgc ggtggtctgc tccatggaga ttacggcccc gttggtgtcc
     3661 gtctgaatgg tcttcaccag agccgcgatc tgcttggttg ccgcgctgga tcgttcagcc
     3721 agccgctgca cctcgtcggc cacaacggca aatcctcgac cggcatcacc agccatggag
     3781 gcctgaatgg cggcgttgag ggagagaatg ttcgtctggt ccgcgatgtc gttgatgaga
     3841 gatacgatgt cgccgatctc ctggctggac tcgccgaggc gcttgatccg cttcgacgtt
     3901 tcctggatct ggccccgaat cacgtccatg ccgtggatgg tgtcctggac cacgtccgat
     3961 cctttgttgg cgatgcccac ggcccgctct gcaaccgatg cagactcggc ggcgttcgag
     4021 gagacgtgat cgatggagac ggccatctcg ttgatcgcct gggacgcggc ggcaatctct
     4081 cgtgcctggc tctcggctgc gccagcgagg ctggatgccg tctcctggct gtcgcccgcg
     4141 gctttagcaa cctgacgcga caggttgttg atgttggata cgagcccgcg catctggtcg
     4201 atggcgaagt tgatcgaatc ggctatcgcg ccggtgaagt tctccgtcac cgaggcctga
     4261 atcctcagat cgccctcacc gagatcggcg atctcgtcga gcagctgaag aatcgcggcc
     4321 tgattgcgtt cgttctgtga cgcctgctca acgagctgcc ggcgcgtctg gctgaccatc
     4381 atcgccgcga tggcggcgac gagcacgata ccggcaacgg cgaacagaag acccgtattg
     4441 ttgatgatgg atgcttcatc agtgacgaca acgtaggcca tccctgcaat cgtgccgacc
     4501 agcaagcaca tcaacgccac tagcaacgga gacaactttc ctgttttcat ttccgtacaa
     4561 cctgattagt tagtcttgtc cgcaacatcg aagaacttct cgtccctcaa tatcgactta
     4621 agatgcgcct cgtaaaaaac gcaccctccc tgatgaaaag caccgcgcaa gtagggcttc
     4681 agtgccgcta cttcctccgc cgtgctttct tcaaaactgt cttctagaaa atgctgaatg
     4741 ccgagcgact gctccaccac gaagcctgcg atcagttcct cgtcttccac taccagcacg
     4801 cgccactgaa ccatgggcgt ggtcgtgcgc atgtcgagga aattgtggag gtcgatgatc
     4861 ggtatgagcc tgccgcggac gttggccacc ccgagcatcc agggtttgac gccgggcagc
     4921 gtcgcgaact tcggcacggc gagaatctcg ctgatctcgc ccattggaga aaccaggcgg
     4981 acaccgccca gctgataacc gacaccctgc cactccagat gctcgttctc ccgggaagga
     5041 agaggcgccg cgttggcgat ggtggtggcc cggatgtccg tgagtatgtt ttgcgcttga
     5101 ccgttcatga caaggattag gcgctcaaag cctcgttgac cgtgctgatc agcgtgcgat
     5161 cgtccacggg tttggtcagg tagccttgcg cgccctggcg tttgccccag acccggtccg
     5221 catcctggct cttggaggag acgatgatca ccggaatgtg gctggtaccc tggcttctcg
     5281 tgagctgccg cgttgcctga aagccgttga taccaggcat cacgacgtcc atcagaatga
     5341 tgtcaggctg ctcttcgttg gccagactga tcccgtcgct gccggaggca gccgtcagca
     5401 cctggtgacc atgacgcgaa agcgcgttgg ccagctggtg cgtctccgag ggagagtcgt
     5461 cgactacgag aatccttgcc atcttctgcg cgtcctgtgc ctgcttccta atggacgagg
     5521 ttccggatcg tgccgagcag ctcgtccttg ctgaagggct tggtgatgta ctgatccgaa
     5581 cccacgatcc ggcctttggc tttgtcgaag aggccgtcct tgctcgagag catgactacg
     5641 ggggtagtct tgaattcgct gttgttcttg atgagcgcgc aggtctggta accgtcaaga
     5701 cgaggcatca tgacgtcgac gaagatgatt cccggccgtg aatcgacgat tttcgcgagc
     5761 gcgtcaaagc cgtccgtggc ggtgatgacg tcataccctt ccttggtcag cagattctcg
     5821 gcgcttcgac gaatggtctt actgtcgtcg attaccagca ccttaacgcc tttgagttcc
     5881 tgttccatat ctggtccttc tacttccgac ttgaccgtcg tgtcaccgtc cattgagtat
     5941 ggcgtcatca aggctgtcgg caggattttc acgtacctac cgaacgctcc cgttgtatca
     6001 tgattgtccc gaaacgatct ataagcgttg gataagaaag ggaattcgtt ctcatttttg
     6061 gggttacgct acgaccgcca agctagttac cgccccagcc ggagcaactt ctgtcccggc
     6121 tcgcccggag caccacagaa cccatgagtc tcaaaatcgc cttcgtgatg gaccctctgc
     6181 ccaccctgtc gctgaagaag gacagcacgc tggcaatgat ccgcgcagcc cagctcaagg
     6241 ggtggcaggt aggctatctc gagcaggggg acctgctgct ccatgagggc gcgccctgcg
     6301 ggatgctccg atccctggag ctcgtcgggc cgtttgccga ttccctcgat ccggccgagg
     6361 ccggcgatcg ctggtatcag ctcagcgaag cggcccttac cccgctcgcc gagctggacg
     6421 tgatcatgat gcgcaaagac ccgccgttcg acatggagta catctactcc acctacctcc
     6481 tcgaacgggc cgaagcgttg ggcgtgctgg tggtcaacaa accccagggg ctgcgcgact
     6541 gcaacgagaa gttctttgcc accgccttcc aggaatgttg ccccccactg gtcgtgaccc
     6601 ggtccgaagc ggcgctgcgc gagtttcaac ggactcacgg caacgtggtg ttcaagcccc
     6661 ttgacggcat ggggggccag ggcgtgttcc gcatcatgga gggcgacgag aacctgggcg
     6721 tcatcatcga gacgctcacg aaaggcggca ccgcccagat catgggccag aagttcattc
     6781 cggagattgc cagcggggac aagcgcgttc tgctcatcga tggcgagccg atcccctacg
     6841 ccctggccag aatccccatg gccggcgagg cgagaggcaa cctggcggcg ggcggcacgg
     6901 gcgagggacg ggagctcacc gagcgcgacc gatggatcgc cgctcaggtc ggtcccgagc
     6961 tgcgtcggcg gggcctgctg ttcgtgggca tggacgtcat cggcgactat ctcacggagg
     7021 taaacgtcac ctgcccgacc tgcatacggg aactcgatgc acagttttcg ctgaatatcg
     7081 gcggtatgct catggacacg atatctgcca agctagatgc tcaggcctga acgccccgtc
     7141 gacaccctgc aaacatgagc gccgccgcca tcactccacg agaccgtctg ggcttcaccc
     7201 tgtttctggc cgtctcgctg cacgccgcca tcattctcgg cgtcagcttc acggcagacc
     7261 tcgacctgct cgaaagccct acggtcgagg tgacgtttgc ccagcacagc gacctcgaag
     7321 cacccgagga tgccgacttc atcgctcaga ccaaccagct cggcagcggc accgagagcg
     7381 aagtgctgga gaccacgacg gaccaggaag cggagttttt cgccaacgag ttcgccgacg
     7441 cgatatccaa ccccttgccg accatcgagc agaaccagct tgactctcgg gagctggtga
     7501 cgaccatctc cgaggtagac gacgtggcgc ccattgaaga agagacgccg gtggaggaaa
     7561 acgtcaatcc gcagccggca accgacattc gcttcgacga tctggcgaag gagatcgcga
     7621 gcctggaagc caggatcgcc gcggaccagc aggccaaggc gaagcagcca agggtgaagc
     7681 ggctcaccag cgtgtccgcc aagtcagcag acgaagccgc ctacctgaac atgtggcgtc
     7741 agaaggtcga gcgcatcggc aacgccaact atcccgacag ccgggtgtac ggcaatctgc
     7801 ggatgctcgt ggtgctgagc tacgacggca cgctcaagga cgtccggatt ctggagtctt
     7861 ccgggcacaa atccctcgac gacgccgcgc ttcgaatcgt ccgtctggct gcgccctacc
     7921 agcagtttcc ggtggagatg cgcaagaagt acgatcagtt ggagatcatt cgcacctgga
     7981 aattctcacg ctccggagcc cgccttggca gctgatacca gcctgaaaaa ccagttcctg
     8041 ctcgccatgc ccggcctggc tggcacctat ttcggggaca cggtcaccta cgtatgcgag
     8101 cacagcgccg acggcgccat gggcctgatg gtcaatcgcc cgacggaagt tacgctgcag
     8161 gcgctgctgg ccgatctcgg catagactcc agcgccgtgt cggcggacat acccgtgatg
     8221 gaaggggggc ccgtggcgag cgagcgcgga ttcgtgctgc acagcgacga ccgagccttt
     8281 gactcttcgc tctccctggg ggacgggctg atgctcaact ccacccgcga ggtgctcgag
     8341 gccattgcgg ccggcgaggg gcccgcggac ttcctggtcg ccatgggata cgccggctgg
     8401 ggcgggggcc agctggagca ggagctgctg gaaaacgcct ggctcacctg ccctggaaac
     8461 accgacgtcc tctttcgcac gcctttcgca gaacgcgtga accaggcggc tgccgcgctg
     8521 ggcatcgatt tccggctgat gtcctcacag gcgggccacg cctgacagcc gcgtgaagca
     8581 gggtcacgtg ctggcgttcg acttcgggct gaagcacatc ggcgtggccg ttggccaatc
     8641 ggttacccga acggccagcc cgctgatcac catcgcggcc agggacggaa cgccggactg
     8701 gtcggacgtg gaacggctga tttccgagtg gcagccggcg gtgctgctgg tgggcctacc
     8761 ccttaacatg gacgacagcg agagcgaaat gtcgctcaag gctcgcgggt tcgccgagcg
     8821 aatgggccgc cggttcggcg ttcccatcga gctggtcgac gagcggctga cttcg
//
LOCUS       DBNHQR010000167         1479 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_142467, whole genome shotgun sequence.
ACCESSION   DBNHQR010000167 DBNHQR010000000
VERSION     DBNHQR010000167.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_142467"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /gene="purT"
                     /locus_tag="AC16VR_03825"
     CDS             <1..268
                     /gene="purT"
                     /locus_tag="AC16VR_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582870.1"
                     /note="non-folate utilizing enzyme, catalyzes the
                     production of beta-formyl glycinamide ribonucleotide from
                     formate, ATP, and beta-GAR and a side reaction producing
                     acetyl phosphate and ADP from acetate and ATP; involved in
                     de novo purine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase 2"
                     /protein_id="HIR6010316.1"
                     /translation="LGLPIPHIQQHGPSASSVVLVEGESRAVRFEGFEAALAEPGTDL
                     RLFGKPEVAGERRMGVALARGRDVDDARKKANAAAAAIRWSFDD"
     gene            563..>1479
                     /locus_tag="AC16VR_03830"
     CDS             563..>1479
                     /locus_tag="AC16VR_03830"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HIR6010317.1"
                     /translation="MPFQKYRAFQAIDLPDRQWPTRTLTTAPRWCSVDLRDGNQALID
                     PMNVAEKLRMFDLLVGVGFAEIEVGFPAASQPDFDFIRRIIDEGRIPDGVAIQVLCQA
                     REELIARTVEALRGAPKVIFHLYNSTSELQRRVVFEMDRSGIIDLAVRGTRLVKAGLE
                     TLDSDITFEYSPESFTGTELDFAVDICTAVAEEWGATPDDPMIINLPSTVEMATPNIY
                     ADQIEWFARNFSRRDSTVISLHTHNDRGTGIAATELALMAGAERVEGTLFGNGERTGN
                     CDIVTMAMNLFSQGVDPELDFRDMPRIRAT"
BASE COUNT          281 a          463 c          477 g          258 t
ORIGIN      
        1 tctcggattg ccgattcctc acatccagca gcacgggccc tcggcatcca gcgtcgtgct
       61 ggtggaggga gaatcgcgcg ccgtcaggtt cgaggggttc gaagccgcgc tcgcggagcc
      121 gggcacggat ttgcgtctgt tcggaaagcc cgaggtagcg ggcgagcggc gcatgggcgt
      181 cgcgctggcc cgggggcggg acgtggacga tgcccgcaag aaggccaacg cggcagccgc
      241 agccattcgg tggtcgtttg acgactgatc agcagggttg gcaaatcctc cccggctggc
      301 tatagtcgcg gccacgattc gggagctgaa ttgaactctc atcaccgtcc aaatctgtct
      361 cttctggcgc tgctgcgact gccggcgtag ccggcaaata cttacgcaaa agggcattcc
      421 gcccacccca tacgaaacct taacgatcct ccgggaccag cagcagcgag aaagacctcg
      481 caggcgaaga acaatgagtg agcaaaccca atcccccttc cgggcccagc gccagccaac
      541 cggcggtcgc gcggcgaaca ccatgccttt ccaaaagtac cgtgcctttc aggccatcga
      601 cctgccggac cggcaatggc cgacccggac cctgaccacg gctccgcggt ggtgcagcgt
      661 ggatctgcgc gacggcaatc aggcgctgat cgacccgatg aacgtggccg agaaactgcg
      721 aatgttcgac ctgctggtcg gtgtcgggtt tgctgagatc gaagtgggtt ttccggccgc
      781 ctcgcagccc gatttcgatt tcattcgccg catcatcgac gagggcagga tcccggacgg
      841 cgtcgccatt caggtgctct gccaggctcg cgaggagctg attgccagaa ccgtggaggc
      901 gcttcgtggc gcgccgaagg tgatctttca cctatacaac tcgacttcgg aattgcagcg
      961 ccgcgtggtg ttcgagatgg accgctccgg gatcatcgac ctggcggtgc ggggaacgcg
     1021 cctggtcaag gcgggcctcg agaccctgga ttccgatatc acgttcgagt actcgccgga
     1081 aagctttacc ggtacggagc tcgactttgc cgtggacatc tgcaccgccg tggccgagga
     1141 gtggggcgcc accccggacg atccgatgat catcaacctg ccgtcgacgg tggagatggc
     1201 cacacccaac atctacgccg atcagatcga gtggttcgca cggaatttct cgaggcggga
     1261 cagcacggtg atcagccttc atacccacaa cgatcgcggc accgggatcg ccgctacgga
     1321 gcttgcgctg atggcgggcg ccgagcgggt tgaagggacg ctgttcggta acggcgagcg
     1381 cacgggaaac tgcgacatcg tcaccatggc gatgaatctc ttcagccagg gcgtggaccc
     1441 cgagctcgac ttccgtgaca tgccgcgtat ccgggcgac
//
LOCUS       DBNHQR010000168         1668 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_522714, whole genome shotgun sequence.
ACCESSION   DBNHQR010000168 DBNHQR010000000
VERSION     DBNHQR010000168.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_522714"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..996)
                     /locus_tag="AC16VR_03835"
     CDS             complement(112..996)
                     /locus_tag="AC16VR_03835"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015251.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR6010318.1"
                     /translation="MGVLAPILLRVWARLMALLPYRATRIVGEAFGSIAYLARSRSAR
                     NTETNLRLCFPDMGDAQIRRLTRRSLQHTGRLLAEAGITFHWSEQKLNQLVTGVEGFQ
                     PLQEALERGRGVLVLVPHLGNWELFALHFGHYGFTALYDPPKIRSLEGLIRDSRQRTG
                     ATLLPIGMQGVRGVLGALKEGRLVSLLPDQVPNPSAGIHAPFFGHPALTMTFAHRLIQ
                     SADPLVVIGACTRQADGFRIGFTAADEAIYSESVEASVAAMNEAIERLVRSDPAQYQW
                     EYKRFKRPPPGVPDPYKS"
     gene            complement(984..1580)
                     /locus_tag="AC16VR_03840"
     CDS             complement(984..1580)
                     /locus_tag="AC16VR_03840"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008940252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HIR6010319.1"
                     /translation="MVKDTADASRCPWCTTDPLYVAYHDLEWGVPEADERALFERLVL
                     EGMQAGLSWLTILRKREHMREAFFAFDPERLARAAEREIQGWLQDPGVIRHRGKLEAM
                     VGNARSLLELDGTFVPLVWSFVEGRPQQNRFRSPLAVPAETPASRAMAKGLKKAGFRF
                     VGPTTCYAFMQSAGLVNDHLTSCPAHERCRSVAESWAF"
BASE COUNT          279 a          533 c          563 g          293 t
ORIGIN      
        1 catgttcgcc atgctcctcg gtcgtctgga gatcttcacc ctactcgtgt tgttcacgcc
       61 cgccttctgg cggcgctgac ccgttgtggg tgagcagacc cggaacacgg ctcaggattt
      121 gtagggatcc ggcacgccgg gcggcggccg cttgaaacgc ttgtattccc actggtattg
      181 cgccgggtcg cttcggacga gccgctcgat cgcctcgttc atggcggcga ccgacgcttc
      241 gacggactcc gagtagatcg cctcgtcggc cgcggtgaag ccgatccgga agccgtctgc
      301 ctgccgggtg caggcgccga tcacgaccag cgggtcggcg ctctggataa ggcggtgggc
      361 aaacgtcatg gtcagggcag gatgaccaaa aaaaggtgcg tggatgcccg cgctcgggtt
      421 gggcacctgg tcgggcaaca ggctcaccag gcgtccctct ttcagcgcgc cgagcacgcc
      481 ccgcaccccc tgcatgccga tgggcagcag cgtcgcgccg gtgcgctggc ggctgtcgcg
      541 gatgaggcct tccaggctgc gtatctttgg cgggtcatac agcgccgtga agccgtagtg
      601 gccgaagtgc agggcgaaca gctcccagtt gcccaggtgc ggcaccagca ccaggacgcc
      661 gcggccgcgt tcaagcgctt cctgcagggg ctggaacccc tccacgcccg ttaccagctg
      721 gttgagcttc tgctccgacc agtgaaatgt gatgcccgct tccgcgagca ggcggcccgt
      781 gtgctgcaag ctgcgtcggg tcaatcgcct gatctgggcg tcgcccatgt cagggaaaca
      841 cagcctgagg ttggtttcgg tgttcctggc ggagcgtgag cgcgccaggt acgcgatgct
      901 gccgaaggct tcaccaacga tgcgggttgc acgatagggc agcagggcca tgagccgagc
      961 ccagacgcgc agcagaatgg gggctagaac gcccaagact ctgcgacgct ccggcagcgc
     1021 tcgtgcgccg gacacgaggt gaggtgatcg ttgacgagtc cagcgctctg catgaaagcg
     1081 tagcaggtgg ttgggcccac gaaacgaaac cccgccttct tcaggccctt cgccatggcc
     1141 cgcgatgctg gggtttccgc gggtacggcg agcggcgagc ggaaccggtt ctgctgcggt
     1201 ctgccttcaa cgaaggacca gaccagcggc acgaatgtcc cgtcgagttc cagcagagat
     1261 cgtgcgttgc cgaccatggc ctccagcttg ccgcgatgcc ggatgacgcc ggggtcctgg
     1321 agccagccct ggatttctcg ctcagccgcg cgggcgagcc gctcgggatc gaaggcgaaa
     1381 aacgcctcgc gcatgtgctc gcgcttgcgg agaatggtca gccaggagag tccagcctgc
     1441 atgccttcca gcaccaggcg ttcgaacagg gcccgctcgt cggcttccgg cacgccccac
     1501 tccaggtcat ggtaagcaac gtagagcgga tccgttgtgc accaggggca gcgggaggcg
     1561 tccgccgtgt ctttgaccat gactgacata tcccttagat ttcgccgcga ttgtaccgaa
     1621 ggcgtgccgg cacgggtgac caggctcatt ctcatcgacc gtgacggg
//
LOCUS       DBNHQR010000169         4905 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1726037, whole genome shotgun sequence.
ACCESSION   DBNHQR010000169 DBNHQR010000000
VERSION     DBNHQR010000169.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4905)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4905)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4905
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1726037"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..1502
                     /locus_tag="AC16VR_03845"
     CDS             51..1502
                     /locus_tag="AC16VR_03845"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HIR6010320.1"
                     /translation="MRLEDQGLLRTGAFVAGQWTAADGGETFDVRNPATGEVVAAVAR
                     CGAAETRRAIEAAERAQVDWRARTAKERARVLRRLYELMMLHQEDLAQILTAEMGKPI
                     VEARGEIAYGASYLEWFAEEAKRVYGDTIPGPAPDKRIVTIKQPVGVVGCITPWNFPN
                     AMLTRKVGPALAAGCAVVCKPATATPLSALAIAELGARAGVPAGVLNVLAGSTREIGS
                     EITGNPAVRKLTFTGSTGVGKALIEQCAATVKRTSMELGGNAPFIVFDDADVDAAVAG
                     AIASKYRNAGQTCVCANRLLVQAGVYEEFAEKLSAAVAEFRLGDGAEETTTIGPLIDA
                     GAADEVLAMIDDAIANGASAVIGGARAAQGGSFVQPTVLTEVTPRMRVFREEIFGPVA
                     PLIRFETEDEAIRLANDTEFGLASYFYARDVGRIWRVAEGLEYGIVGINEGIVSNEMA
                     PFGGVKESGNGREGSKYGMDDYLEIKYLCMGGI"
     gene            1517..3658
                     /locus_tag="AC16VR_03850"
     CDS             1517..3658
                     /locus_tag="AC16VR_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010321.1"
                     /translation="MWFHEKAKHRPVSLGPYPLETLPRDPAQSEIELRMPLARQLPLE
                     ESASELGAAAGRYLSLFLEFRDGEVAPQPAPVPDDVDLRADDVKGLAYFFDADHVGIC
                     ELPSGARLIESDALDHAMAVVILVRFGRQPEADNLARDWIAGAEHDVAELRAAEIAVT
                     VAGYIRQLGYPATAHVRRATTVDLDRLAVLSGVCVRRDGAVCSPYFGDGFAAAAVTTD
                     YALAVDAPLAPDQGKARGRRYHRGQNGAVSGRERRRRDRRASHLSRYPMEQVRRVERP
                     TTLIIDDEVPRVPKRANFFTRAAFGDLGEKSQREVRRFAYKHPSTAGTMQLIRSMVSL
                     QDGEIAGSRWASLDSKANAAAIKSLGYHLGADLVGICEIPPYAWYSHQADGTLIEPYH
                     RFAVVMLIDQGFDTMEGASGDDWISGAQSMRGYVRGAEIAGVMAEFLRSVGVSARPQT
                     NADSDVLHIPLILWAGLGELSRIGELVLNPFVGPRFKSVVLTTDLPLEVDAPIDFGLQ
                     YFCNHCLKCARECPCNAIPTGSKVMFNGYEIWKPDVERCTRYRITNQKGAACGRCMKT
                     CPLNKVVGADGALAHRAGNWLAIHLPWLRPLLVPIATRLDDRTGYGARNPVKKWWFDL
                     EIVDGVCVHAEANQRDLDVGRSAASRRDTVGYHPADTMPAPDAQGPVPVNHREAAGRS
                     ALLETPADARRRKRKGEPGPSHYVPLVDVTD"
     gene            3759..4214
                     /locus_tag="AC16VR_03855"
     CDS             3759..4214
                     /locus_tag="AC16VR_03855"
                     /inference="COORDINATES: protein motif:HMM:NF047172.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR6010322.1"
                     /translation="MNDFDRSLPMLLYRALDLIMPRFRAIFGGFGLTEQQWRVLRVLW
                     QRDGRALLELADATLISAPSMVGVVDRLARSGLVERRRSQSDRRVVRVFLTGRGRDLE
                     EQVTPLVDAAYADLRGSVGEGDWEVLLGALARLNAGLADLDRIDEVVNR"
     gene            4239..>4905
                     /locus_tag="AC16VR_03860"
     CDS             4239..>4905
                     /locus_tag="AC16VR_03860"
                     /inference="COORDINATES: protein motif:HMM:NF023222.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6010323.1"
                     /translation="MGIRTGAQYLEGLRDGREVWMHGRRIEDVTTEPGLCRGAHTLAD
                     FLDRVHEPAHREKLSYAEAGSDERFSLSFLKPGSREDVERRGAAFYEWAKWSGGMFGR
                     TPDYKNASVMAFAAASEFLAGEGGRPEYAENMRRYYEKARVEDLVLTHTLVNPQFNAA
                     LAAAGRSSPEVALQIVGENDAGVVVNGARLLATLGPHADEIEVFPSTLLKASPENVPF
                     AIAF"
BASE COUNT          822 a         1526 c         1712 g          845 t
ORIGIN      
        1 accgatggcg cgttagaatc gatggtacgc caatcaggag ggattgaacg gtgcgcctag
       61 aagatcaggg tctgctgcgg accggagcgt ttgtagccgg acagtggacg gcggcagacg
      121 gcggcgaaac cttcgacgtt cgcaatccgg caaccggaga agtggttgcc gcggtggcgc
      181 ggtgcggcgc cgcggagacc cgccgtgcca tcgaggcagc ggagcgtgct caagtggact
      241 ggcgggcgag aacggccaag gagcgtgcgc gcgtgctgcg ccggctctac gagttgatga
      301 tgctgcatca ggaggatctt gcgcagattc ttaccgccga gatgggcaag cccatcgttg
      361 aagcgcgcgg cgagatcgcc tacggggcaa gctatctgga atggttcgcc gaagaggcca
      421 agcgcgttta cggcgacacc atcccgggcc ccgcgccgga caagcggatc gtcaccatca
      481 agcagcccgt gggcgtcgtc ggctgcatca ccccgtggaa tttccccaat gccatgctga
      541 cccgcaaggt cgggcccgcc ctcgccgccg gctgcgcggt ggtgtgcaag ccggccaccg
      601 cgacgccgct gtctgcactg gccatcgcgg agcttggggc gcgggccggc gtgccggcgg
      661 gtgttctgaa cgtgctggcc ggaagcaccc gggaaattgg cagcgagatc accggcaacc
      721 cggcggtgcg caagctgacc ttcaccggat ccaccggcgt gggcaaagcg ctcatcgagc
      781 agtgcgcggc cacggtcaag cgcacgtcca tggagctcgg gggcaacgcg cccttcatcg
      841 tcttcgacga cgcggacgtg gacgccgccg tcgcgggggc catcgcatcc aagtaccgaa
      901 acgcgggcca gacgtgcgtg tgcgccaacc gcctgctcgt ccaggccggg gtatacgagg
      961 aatttgcgga gaagctctcg gcggcggtgg cggagttccg gctgggcgac ggggcagagg
     1021 agaccaccac catcggtccc ctcatcgatg caggggctgc cgacgaggtg ctggcaatga
     1081 tcgacgacgc gatcgccaac ggcgccagcg cggtgatcgg cggggcgcgg gccgcccagg
     1141 ggggctcctt cgtccagcca accgttctca cggaagtgac cccgcgcatg cgggtgtttc
     1201 gcgaagagat cttcgggccg gtggcgccgc tcatccggtt cgagacggag gacgaagcga
     1261 tccggctggc caacgatacc gagttcgggc tggcgtcgta tttctacgcc cgggacgtgg
     1321 gtcgaatctg gcgcgtcgcc gagggactcg aatacggtat cgtcgggatc aacgagggca
     1381 tcgtctcgaa cgagatggcg cctttcggcg gggtcaagga gtcgggtaac ggccgcgagg
     1441 gatcgaagta cgggatggac gactatctgg agatcaaata tctctgcatg ggcgggatct
     1501 agcggggctc gccgccatgt ggtttcacga gaaggcaaag catcggcccg tgagcctcgg
     1561 cccctatccc ctggagacgt tgccccggga tccggctcag agcgagatcg agctgcgcat
     1621 gccgctggca aggcagctgc cgctggaaga gtcggcgtct gagcttggcg cggccgccgg
     1681 gcgctacctc tcgctcttcc tggagtttcg cgacggcgag gtggctcctc agcccgcgcc
     1741 cgtccccgac gacgtcgacc tccgtgcgga cgacgtcaaa ggtctggcct acttcttcga
     1801 tgcggatcac gtgggtatct gcgagctccc atcgggcgca cgactgattg aatctgatgc
     1861 cctggatcac gccatggccg tcgtcatcct cgttcgcttc gggcgccagc cggaagccga
     1921 caacctcgcc cgggactgga ttgcgggcgc cgagcacgat gtggcggagc tcagagcggc
     1981 ggagatcgcc gtcacggtgg ccggctacat ccggcagctg ggctacccgg ccaccgctca
     2041 cgtccggcgg gccaccaccg tggacctgga ccggctggcc gtcctgtccg gcgtctgcgt
     2101 gcgcagggac ggtgccgtct gctccccgta ctttggtgac ggcttcgctg cagccgcggt
     2161 gaccacggat tacgccctgg cggtggatgc ccctctggcc ccggaccagg gcaaggcccg
     2221 gggccggcgc taccaccggg gtcaaaacgg cgcggtctcc ggcagagaac gccgtcgccg
     2281 ggaccggcgt gcgtctcact tgagccgcta tcccatggag caggtccgtc gggtcgaacg
     2341 gccgaccacg ctgatcatcg acgatgaggt tcccagggtt ccgaagcggg cgaacttttt
     2401 tacgcgggcg gcttttggcg accttggcga gaagtcccag cgcgaggtcc gccggtttgc
     2461 ctacaagcac ccttccacgg cggggaccat gcagctcatc aggagcatgg tgtcgttgca
     2521 ggacggggag atcgccggtt cccgttgggc ctcgctggac tcgaaagcca acgctgcggc
     2581 cataaagtcg ctcggctacc acctcggggc ggatctcgtg ggcatctgcg agattccgcc
     2641 gtatgcctgg tactcccatc aggcagacgg aacgctcatc gagccgtatc accgctttgc
     2701 cgtggtgatg ctcatcgatc agggcttcga caccatggag ggcgccagcg gggacgactg
     2761 gatttccggt gcgcagtcca tgcgaggcta cgtgcgtggt gcggaaatcg ctggcgtgat
     2821 ggccgagttt ctgcgctccg tgggagtctc ggcgcgaccg cagaccaacg cggacagcga
     2881 cgttctgcac ataccgctga ttctgtgggc gggccttggc gagctcagcc gaatcggcga
     2941 gctggtgctg aacccgttcg tcggtccccg tttcaagtcc gtggtgctga ccacggatct
     3001 gcccctggag gtggacgcgc ccatagactt cgggctgcag tatttctgca atcattgcct
     3061 gaagtgcgcg cgggaatgtc cctgcaacgc gattcctacg ggctccaagg tcatgttcaa
     3121 cgggtacgag atctggaagc cggacgtgga gcggtgcacg cgctaccgca ttaccaacca
     3181 gaaaggggcg gcctgcggaa ggtgcatgaa gacctgcccg ctcaacaagg tagtgggcgc
     3241 cgatggcgct cttgcgcacc gagccggcaa ctggctggca attcatcttc cctggctgcg
     3301 gccgctcctg gttcccatcg ccacgcggct ggatgaccgg accggctacg gggcccgtaa
     3361 cccggtgaag aagtggtggt tcgatctgga gatcgtcgac ggcgtatgcg tccatgcaga
     3421 agcgaaccag cgcgacctcg acgtcggtcg gtcggcggct tcccggcgcg ataccgtggg
     3481 ctaccatccg gcggatacga tgccggctcc cgatgctcag ggtcccgttc ccgtcaacca
     3541 ccgggaagcc gctggccgga gcgccttgct ggaaacccct gccgatgcgc gtcgccggaa
     3601 acgcaaaggc gagcctggcc cctctcatta cgttccgctc gtcgacgtca ccgattgacc
     3661 ccaccgcttt tcggccccct tgcagccgat gattacccgg ttgacgcgtc tcgagattta
     3721 actatcattt taattattaa tacatgaact aaaagcgcgt gaacgacttc gaccgatccc
     3781 tgcccatgct gctttaccgg gcccttgacc tgatcatgcc cagatttcgc gccatattcg
     3841 gcgggttcgg gctcactgag cagcagtggc gcgtgctgcg ggtgctgtgg cagagggatg
     3901 gtcgtgcgct gctcgaactt gccgacgcca ccctgatctc ggcgcccagc atggtcgggg
     3961 tggtggatcg cctggcgcgc agcggtctgg tggaacgacg ccggtcgcag agtgatcggc
     4021 gggtcgtgcg ggtgtttctc accgggcgcg gcagggactt agaggaacag gtcacgcccc
     4081 tggtggacgc ggcctacgcg gacctgcgcg ggagcgtggg ggagggtgac tgggaggttc
     4141 tgctcggcgc gctggcccgg ctgaatgcag ggcttgctga cctggaccgg atcgacgagg
     4201 tggtcaatcg ctgaaccggc gaagcgagga gatttacgat gggaatcaga acgggtgcgc
     4261 aatacctcga agggctcaga gacggccgtg aggtctggat gcatggcagg cggatagagg
     4321 acgtgacgac cgagccgggg ctctgccgcg gtgcccacac gctggcggat tttctggatc
     4381 gggttcacga gcctgcccat cgcgaaaaac tctcctatgc cgaggcgggt agcgacgagc
     4441 gattcagcct gtccttcctc aagcccgggt ccagggagga cgtggagcgc cgaggtgccg
     4501 cgttctacga atgggcgaag tggtcgggcg gcatgttcgg acgcacgccc gactacaaga
     4561 acgcctcggt gatggcgttt gccgcggcgt cggaatttct cgccggggag ggcgggcgac
     4621 cggagtacgc ggagaacatg cgtcgctact acgaaaaggc ccgtgtggag gacctggtgc
     4681 tcacccacac gctggtcaat ccgcagttca acgcggcgct tgcggcggcg ggtcgatcga
     4741 gtcccgaggt ggccctgcag atcgtgggcg agaacgatgc cggcgtggtc gtcaacggcg
     4801 cgcggctgct tgccacgctg ggcccccatg cggacgagat cgaggtgttt ccgtccaccc
     4861 tcctgaaggc ctcgccggag aacgtgccct ttgccatcgc tttca
//
LOCUS       DBNHQR010000170         2503 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_649610, whole genome shotgun sequence.
ACCESSION   DBNHQR010000170 DBNHQR010000000
VERSION     DBNHQR010000170.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_649610"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..104)
                     /locus_tag="AC16VR_03865"
     CDS             complement(<1..104)
                     /locus_tag="AC16VR_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016491512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR6010324.1"
                     /translation="MTRRILVTSALPNANGAIHIGHLLEHIQTDIWVR"
     gene            233..1258
                     /gene="apbC"
                     /locus_tag="AC16VR_03870"
     CDS             233..1258
                     /gene="apbC"
                     /locus_tag="AC16VR_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009876885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HIR6010325.1"
                     /translation="MKIEQFPDPYLNVSWGELGARVLSSGNQVAVTLGYPAAGIRDRL
                     AGQLADFLGVSAVDLDLRFQAPPGRGFSRVKHIIAVASGKGGVGKSTTAVNLALALDR
                     EGARTGLLDADIYGPSQGMMLGVAEGRRPEVRDQQYFEPIKAHGIQAMSMSFLVNEDT
                     PMVWRGPMVSGALQQLLNQSLWDDLDYLVVDMPPGTGDIQLTLSQKVPVSGAVIVTTP
                     QDIALLDAKKGIEMFRKVDIPVLGVVENMSVFRCPHCGGESAVFGTGGGARIAAETGV
                     PVLAELPLAMQIREQADGGLPTVVQDPTGEIAGLYREAARHLAARLWGIEARADADMP
                     EIRITDD"
     gene            1294..1857
                     /gene="dcd"
                     /locus_tag="AC16VR_03875"
     CDS             1294..1857
                     /gene="dcd"
                     /locus_tag="AC16VR_03875"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HIR6010326.1"
                     /translation="MTIKSDRWIREMAAQGMIEPFEPQQVRLRGDEKVISYGTSSYGY
                     DVRCARNFKVFTNIYSATVDPKAFDERSFVDVTGDVCIVPPNSFALASTVEYFRIPED
                     VLTICLGKSTYARCGIIVNVTPLEPEWEGHVTLEFSNTTNLPAKIYANEGVAQMLFFQ
                     SDERCEITYKDRGGKYQGQRGVTLPRP"
     gene            complement(1854..>2503)
                     /gene="purM"
                     /locus_tag="AC16VR_03880"
     CDS             complement(1854..>2503)
                     /gene="purM"
                     /locus_tag="AC16VR_03880"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HIR6010327.1"
                     /translation="GCALVGGETAEMPGFYAAGDYDLAGFCVGVVEREDIVTGREIAP
                     GDGLIGLPSSGPHSNGYSLIRRIIEERTVHVDGELLDQLMAPTTIYAKQVLALAEAIP
                     VRGMAHITGGGLLENLPRMFAAADVAALVDMDSWTRPEVFSWLQQAGNVEEREMLRTF
                     NCGIGYVICVAGEDTAAALSTLTDLGSSPTVIGRVVPAGTEAGQGQLLIAPEVEP"
BASE COUNT          438 a          796 c          836 g          433 t
ORIGIN      
        1 aatcgcaccc agatgtcggt ctggatgtgc tccagcagat gaccgatgtg aatcgcgccg
       61 ttggcgttgg gcaaggcgct ggtgaccagg atgcgacgtg tcatggttgg ctgcgcgtga
      121 gcgcttcggg gtgtccgaaa aagcgggact ataacggtaa ctcacctata atgcgcgcct
      181 tcggcgtctt accacaggca gggccccgca agggcgctgc cgcggtgcat taatgaaaat
      241 cgagcaattt ccggatccgt atctcaacgt ttcgtggggc gagctgggcg ccagggtgct
      301 cagctcgggc aaccaggttg ctgtcaccct gggctacccc gcggccggta tccgcgaccg
      361 gcttgccggg cagctggccg attttctcgg ggtgagtgcc gtcgatctcg atctgcggtt
      421 tcaggcacct ccggggagag gattcagccg ggtcaagcac atcattgccg ttgcctcggg
      481 caagggcggc gtaggcaagt ccaccaccgc cgtgaatctg gcgctggcgc tggatcggga
      541 aggcgcgcga acgggtctgc tcgacgccga catttacggc cccagccagg ggatgatgct
      601 cggcgtcgcc gagggtcggc ggcccgaggt cagggaccag cagtacttcg agcccatcaa
      661 ggcccatgga atccaggcca tgtccatgag cttcctggtg aacgaggaca cgcccatggt
      721 ctggcgcggg cccatggttt ccggcgcgtt gcagcagctg ctcaaccagt cgctgtggga
      781 cgatctggac tacctcgtgg tggatatgcc gccgggaacc ggcgacatcc agctgacgct
      841 gtcccagaag gtgcccgtgt cgggcgccgt gatcgtcacc acgcctcagg acatcgccct
      901 gctggatgcc aagaaaggca tcgagatgtt ccgcaaggtc gacattcccg tgctcggcgt
      961 ggtcgaaaac atgagcgtct tccgctgccc gcactgcggc ggggagagcg ccgtgttcgg
     1021 taccggaggc ggggcgcgga ttgctgccga gaccggtgtg cccgtgcttg ccgagctgcc
     1081 gctggccatg cagatccggg agcaggcgga cggtggcctg ccgaccgttg tgcaggatcc
     1141 gacgggcgag attgccggtc tgtaccggga ggccgcgcgg catctcgcgg cgcggctgtg
     1201 gggcattgag gcacgtgcgg acgccgacat gcccgagatc cgcatcacgg acgactagac
     1261 catggcccaa ccacagcaga tcggggtggc acggtgacga tcaagtcgga tcgctggatt
     1321 cgcgagatgg cggcgcaggg catgatcgaa cccttcgagc cgcagcaggt gcgcctcagg
     1381 ggcgatgaaa aggtcatttc ctacgggacg tccagctatg gctacgatgt ccgctgcgcg
     1441 cggaacttca aggtcttcac gaacatctac tccgccaccg tcgaccccaa ggcgttcgac
     1501 gagcgaagct tcgtggacgt gaccggggac gtctgcatcg ttcccccgaa ctcgttcgcg
     1561 ctggccagca cggtagagta cttccggatt cccgaggatg tgctcaccat ctgtctgggc
     1621 aagtcgacct acgcccgctg cggcatcatc gtcaacgtca ccccgctcga acccgagtgg
     1681 gaaggccacg tcacgctgga gttctccaac acgaccaacc tgccggcgaa gatctatgcc
     1741 aacgaaggtg tagcgcagat gctctttttt cagtctgacg agcgctgcga gatcacctac
     1801 aaggaccgcg gcggcaagta tcagggtcag cgcggcgtga ccctgcccag accctagggc
     1861 tcgacctcgg gcgcgatgag aagctgaccc tgaccggcct cggttcccgc cgggaccacg
     1921 cggccgatga cggtgggcga agaacccaga tccgtcaggg tcgacagggc tgctgcggtg
     1981 tcctctccgg caacgcagat cacgtagccg atgccgcagt tgaacgttct cagcatctcc
     2041 cgctcctcga cgttgccggc ctgctgcagc cagctgaaca cctccggccg ggtccacgag
     2101 tccatgtcca ccagcgccgc aacgtcggcg gcggcgaaca ttcggggcag gttctcgaga
     2161 aggccgccgc cggtgatgtg ggccatgccg cgcacgggga tggcttctgc cagcgcgagc
     2221 acctgcttgg cgtagatggt ggtcggcgcc atgagctggt ctagcagctc accatccacg
     2281 tggacggttc gctcctcgat gatccgacgg atgagcgagt atccattgga atgggggccg
     2341 ctgctcggca gcccgatcaa cccgtcgcca ggcgcgatct ctctgccggt gacgatgtcc
     2401 tcgcgctcga cgaccccgac gcagaacccg gccaggtcgt agtcgccggc cgcgtaaaac
     2461 ccgggcattt cggcggtctc gccgcccacc agggcgcagc cgg
//
LOCUS       DBNHQR010000171         2164 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1726204, whole genome shotgun sequence.
ACCESSION   DBNHQR010000171 DBNHQR010000000
VERSION     DBNHQR010000171.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1726204"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..720)
                     /locus_tag="AC16VR_03885"
     CDS             complement(154..720)
                     /locus_tag="AC16VR_03885"
                     /inference="COORDINATES: protein motif:HMM:NF018508.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HIR6010328.1"
                     /translation="MPLKVTFELEDKDLKFFRSIIKQARETAQSVSEADIASKAEEMI
                     AEVRDGSVPSFVVERVNKVQSLVDMLRDVEWDLAAQERKKVVAALAYFADPHDLIPDN
                     VPVLGYIDDAIMIELVVSELKHEIDSFLDFCKYRKEEKARNRNPNLSREDYLGIKRRE
                     LHDRMRRRRRSKTSPVRGGTRTRLRLLG"
     gene            complement(789..2144)
                     /locus_tag="AC16VR_03890"
     CDS             complement(789..2144)
                     /locus_tag="AC16VR_03890"
                     /inference="COORDINATES: protein motif:HMM:NF013406.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HIR6010329.1"
                     /translation="MSSASWTAGSAAPCTFPFFCSASGFSSPSISVFLRFDSFAIYLG
                     GPAALFWMWVTAFLGMTTKFVEVTISHRYRVKDAQGRMAGGPMYYMERGLNMRWLAVF
                     FAAATVVSSFGSGNLPQSNNMASGLEASFGIPTWVAGAVLAALLGLVIIGGIRRIAVV
                     AASIVPVMGVLYALGALSVAASNADQIVPSFAAVFSNAFTGSAATGGFLGATFAYAFN
                     RGVNRGLFSNEAGQGSAPIAHASAGTYKEGNPAHVKALFDHLDIVPPANDPVTAFSGS
                     VLVSDGVASSAAFTVLHNRSIAEDVRFQSAGRPFSGVLEVVDGSLVTPDVDIRGRSLL
                     HSVPLTAEAFKRSFLGEYGQYAVTVGLVMFAFSTALAWSYYGDRAVTYLFGVQWVSVY
                     RMVYVAGFFAATVVDTSLVWLISAVTLALMTIPNLIGLVLMRREVKSLTDDYWRRVSQ
                     R"
BASE COUNT          425 a          702 c          680 g          357 t
ORIGIN      
        1 aattgggtga aagcgtcgag atcgacctgt ttccggagcc gccccaggtg ttcgccctgc
       61 tcgagcgagg cgcttcgctg attcgactac tcgacgatcc gtcccaccgc tacgcgcttt
      121 gcgaggcctg caccgtttcc tggtagccga cgatcagccc agcagccgga gccgggtccg
      181 ggtaccgccg cggacgggac tcgtcttcga gcgccggcgc cggcgcatgc ggtcgtgcag
      241 ctcgcgccgt ttgatgccca ggtagtcctc gcgactcaga ttcgggttgc ggttccgcgc
      301 cttttcctct ttgcggtact tgcagaagtc gaggaacgag tcgatctcgt gcttcagctc
      361 gctgaccacc agctcgatca tgatcgcgtc gtcgatgtag ccgagcacgg gcacgttgtc
      421 cggaatcaga tcgtgggggt cggcgaagta cgccagcgcc gccaccacct tcttgcgttc
      481 ctgggccgcc agatcccact ccacgtcgcg aagcatgtcg accagggact gcaccttatt
      541 gacacgctcc accacgaacg acgggaccga gccgtcccga acctcggcga tcatctcctc
      601 ggccttgctc gcaatgtctg cctcgctcac gctctgggcg gtctcccggg cctgcttgat
      661 gatggaccgg aagaatttca ggtccttgtc ttcgagctcg aaagttactt tcagtggcat
      721 gggcgcctcg ccggtgtctg gtggttggtt agatgcagcg cgactatacc gaatttaacg
      781 cgtgatgatt accgctggga tacccggcgc cagtagtcgt cggtcaacga cttcacttcc
      841 cggcgcatca gcacgagacc gatgaggttg gggatcgtca tcagggcgag cgtcaccgct
      901 gatatcagcc agaccagaga cgtatcgacc accgtggcgg cgaagaaccc cgccacgtag
      961 accatgcggt atacgctcac ccactgaaca ccgaacaggt aggtcacggc acggtcaccg
     1021 taataagacc aggcgagcgc ggtggagaac gcgaacataa ccaggcccac cgtgaccgcg
     1081 tactgcccat actcgcccag gaaactgcgt ttgaaggctt ctgcggtcag cggcacggag
     1141 tgcaggagcg atcggcctct gatgtcgaca tcgggagtca ccaggcttcc gtcgacgacc
     1201 tcgaggacgc cgctgaaagg cctgccggcg ctctgaaaac gcacgtcttc cgcgatggag
     1261 cggttgtgga gcacggtgaa cgcggcactc gacgcgaccc cgtcgctcac cagcacggac
     1321 ccggagaacg ccgtcaccgg gtcattcgcc ggcggcacga tgtccagatg atcgaacaac
     1381 gccttgacgt gggcagggtt gccctccttg taggtgccgg ccgaggcgtg cgcgatgggc
     1441 gcggacccct gccccgcctc gttcgagaac aaaccgcggt tgacgccccg gttgaaggcg
     1501 taggcaaacg tggcgcccag gaagccgccc gtcgccgccg aaccggtgaa ggcgttggag
     1561 aacaccgctg cgaaggaggg aacgatctga tccgcgttgc ttgcggccac cgaaagcgcc
     1621 cccagcgcgt agagcacccc catgacgggc acgatgctgg cagccaccac ggcgattcgc
     1681 cggataccgc cgatgatgac gagcccgagc agggcggcaa gcactgcccc ggcaacccag
     1741 gtgggaatgc cgaacgacgc ctccaggccc gaggccatgt tgttgctctg gggcaggttg
     1801 ccggatccga acgagctcac caccgtggcc gcggcgaaaa acacggccag ccagcgcatg
     1861 ttgaggcccc gctccatgta gtacatgggc ccgccggcca tccgtccttg cgcgtccttc
     1921 acccggtagc ggtgggagat ggtcacctcg acgaacttcg tggtcatgcc cagaaaggcc
     1981 gtgacccaca tccagaaaag cgccgccggc ccgcctaggt agatggcgaa agaatcgaat
     2041 ctgaggaaaa ccgagataga gggtgaagaa aagcccgacg ccgagcagaa gaaagggaaa
     2101 gtacacggcg ctgccgagcc agccgtccag gaagccgatg agatcctcga acatggggcc
     2161 gccc
//
LOCUS       DBNHQR010000172         5628 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_523126, whole genome shotgun sequence.
ACCESSION   DBNHQR010000172 DBNHQR010000000
VERSION     DBNHQR010000172.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5628)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5628)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5628
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_523126"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="AC16VR_03895"
     CDS             <1..208
                     /locus_tag="AC16VR_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010330.1"
                     /translation="VRFTPYPFEDVAQLVRESYPELYLFSSDYPHAEGGRDPIGRFDA
                     ATATLTDSERAQFFSRNFTDLYRQ"
     gene            complement(186..1100)
                     /locus_tag="AC16VR_03900"
     CDS             complement(186..1100)
                     /locus_tag="AC16VR_03900"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HIR6010331.1"
                     /translation="MESIRRALEDDLSLFAVPVFIVAVLFEALWSRGTRRRLYGVQDS
                     LVSLSMLVASAVVEILPRLLAVALMIQLHEMSPLRDVVVRQWWAWALLFVLDDFSYYW
                     FHRVNHEVRLLWAGHVNHHSSEYLNFATALRQGVGERIHKMVFWLWLPLLGFDVGMIL
                     TMIGLNLIYQFWVHTQAVDRLPAWVELIFNTPSHHRVHHASNVRYLDCNHAGVLIVWD
                     RLFGTFSEETAEDPPVYGLTRNVRSFNPAVVLFHEYAALWRDVRRAPGWRQKLGYLLR
                     APGWSHDGEDRRSRTLRRCEAAIAGTGP"
     gene            complement(1165..2007)
                     /locus_tag="AC16VR_03905"
     CDS             complement(1165..2007)
                     /locus_tag="AC16VR_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6010332.1"
                     /translation="MAETRKVFVVLDPTTMEQASLQWGEKIALDNRAHRSQDTHLHVY
                     CCVNFKSVTAVAADTFEHVEAATLERVREWVNRIVTHTRSLGVPVDVEVEWDADWRKA
                     IVGAVRRQNPDVVIKNLTQHARLVRLVRDTSDWQLLRDCQCPVLLIKTGRLYGLKKIL
                     VAVKHTEDEEYQAANRGIMARAQGLADDFGAELHAVTCYDPDRRPDRQRFADSFGLQR
                     NQVHAAMGKPEKVLASVAEEIRADMLIIARVARPESTGLLGDTARRVIDEIATDVLVL
                     PMNG"
     gene            2105..3319
                     /locus_tag="AC16VR_03910"
     CDS             2105..3319
                     /locus_tag="AC16VR_03910"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase family protein"
                     /protein_id="HIR6010333.1"
                     /translation="MERICEISPLAHLPDDRMPNADPFAPEIPEPVLEDLRRRLTTIR
                     WPHVIGEDDWVYGVPQAWLKGMADYWAHEWNWNSTATAMAAFDHYLADMDGIPVHFLH
                     VPGKGPAPKPLILTHGWPWTFWDYKDVIGPLSDPAAFGGDAADAFDVVVPSLPGFGFS
                     TPLTSAGIDVAEVARLWVRLMQDVCGYERFAAHGGDWGALVTAHLGHAHAQSLIGAHL
                     ALPLIPGVDRASVPREAYAEDEQWMLERAAQSGPLIRSHVSVHTTDPQTLAYAMSDSP
                     IGTAAWIWERRRNWSDCDGDVERAFDRDHLCTTAALYWCTGAFTSSLRLYFEHFNKPW
                     PLVHDRKPTVEAPTGFAIFPKDVVQMPRRVAAEHTDLRRWSVMPRGGHFGAAEAPDLI
                     VDEIRSFFRDLS"
     gene            3336..4730
                     /locus_tag="AC16VR_03915"
     CDS             3336..4730
                     /locus_tag="AC16VR_03915"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6010334.1"
                     /translation="MAKHHAQDLSRPSDPNLAPGAARCPGPSFQDIVRTEDSARSTAL
                     TTQSYDFLGDEDVPFSRYTSREMYELEIERVWSRTWQWACREEHIPSPGDYHVYDVGP
                     YSVLVIRSDPNTVRAFVNSCPHRGMQFCDSGTSGRGKQFLRCPFHGMSWHLDGSLREI
                     PCRWDFPHVEEENFGLTPVACETWGGFVFISLDPEPEPLATYLGVLPEHFSEWPLEER
                     YVALHMQKVLPGNWKMCMEGFMEAYHVMATHPEGLKTSAWATTQYDVFGRHVSRFMQP
                     RAMPSPYYGRPVSEREAFAALGHDPEDFPEGSSARQAHADSVRRSMGESLGVDLTGYT
                     NSQLLDSIEYHLFPNACLFPGIIVPFIYRFRPLTWDSCLHDILVLHPVPDSGPRPPPA
                     EPIRLGIDDSYTSVPGFSENRLALVLDQDTDNFRRQWAGMQASRKPGETLANYQEVRI
                     RHFHQTLDAYLASA"
     gene            complement(4727..>5628)
                     /locus_tag="AC16VR_03920"
     CDS             complement(4727..>5628)
                     /locus_tag="AC16VR_03920"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6010335.1"
                     /translation="TRRWVEAQERAIREIASDLIDAVESRGEMDFISEFAVKIPLIVI
                     ADALGVPREDIATFRHWSDDILAGNLDVLSHERRREVATSWVEATRYFADTVEARRRN
                     PRDDLISSLATAEIDGERLEVAEILPIISTLLLAGNETTANLIGNGMRILLEQPEVMA
                     RLREDAGLIPPFLEEVMRFDAPVQCLYRVVTRDTELGGVTIPAGSNVMLGWGSAGHDP
                     DFFERPEQFDLERENVNQHVGFGYGPHLCVGLMLARAEARIAFETVLDRLSGVRLKPG
                     SELEYVPTYATRGLQRLDLLFDA"
BASE COUNT         1034 a         1881 c         1704 g         1009 t
ORIGIN      
        1 ggtgcggttt acgccctatc ctttcgagga cgtcgcccaa ctcgtccgtg agtcctatcc
       61 cgagctgtac ctgttctcat cggattaccc tcatgccgag ggcggacgcg accccatcgg
      121 ccgcttcgac gcggccaccg ccacgctgac cgattccgag cgagcccagt tcttctcgcg
      181 caacttcacg gacctgtacc ggcaatagcg gcctcgcaac gccgcagcgt ccgggaacgc
      241 cggtcctcgc cgtcgtggct ccagccggga gcccggagaa gatagccgag cttctggcgc
      301 catccaggcg cgcgccggac gtcacgccac aaggccgcgt actcgtggaa caggaccacg
      361 gcaggattga aagagcgaac gttccgcgtc agcccgtaca cgggcggatc ctccgccgtc
      421 tcctcgctga acgtcccgaa cagtcgatcc cagacgatca gcacacccgc atggttgcag
      481 tccaggtagc ggacgttgct ggcgtgatga acccggtgat gcgaaggcgt gttgaagatg
      541 agctccaccc aggcgggaag ccgatccacg gcttgcgtgt gcacccagaa ctgatagatc
      601 aggttgagcc cgatcatggt caggatcatg ccgacgtcga atccgagcag cggcagccaa
      661 agccagaaga ccatcttgtg aatgcgctcc ccgacgccct gccggagggc ggtggcgaag
      721 ttcagatact cagaggagtg atgattgacg tggcctgccc agagcagacg cacctcgtga
      781 ttgacccggt gaaaccagta ataagaaaag tcatccagaa cgaacagcag cgcccacgcc
      841 caccactggc gcaccacgac gtcccgcagc ggactcatct cgtgcagctg aatcatcagc
      901 gccacggcaa gaagacgggg cagaatctcc accacggcgg acgccaccag catgctcagg
      961 gataccagag agtcctgcac cccgtaaaga cggcgccggg tgccacggga ccacagtgct
     1021 tcaaaaagca ccgccacgat gaacaccggc accgcgaaaa ggctcagatc gtcttcaagt
     1081 gctcgcctga tgctctccac gatatccctg cccgaactcg atgggccgat tatatcgaac
     1141 ggggttcgcc cccgggggtc cggatcaccc gttcatgggg agcacgagaa cgtccgttgc
     1201 gatctcgtcg atgacccgac gcgcggtgtc gccgagcagc cccgtagact cgggccttgc
     1261 gaccctggcg atgatcagca tgtccgcacg gatctcctcg gccacggatg ccagcacttt
     1321 ttccggtttg cccatggccg cgtgcacctg gttacgctgc agaccaaagc tgtcggcaaa
     1381 acgctgcctg tcgggtcgcc gatccgggtc gtagcaggtc accgcgtgca gctccgctcc
     1441 gaaatcgtcg gcaagtccct gtgcccgggc catgattccc cgattggcgg cctggtactc
     1501 ctcgtcttcg gtgtgcttga ccgccaccag aatctttttg aggccgtaga gccgaccggt
     1561 cttgatcagg agcaccggac actggcaatc gcgcagcagc tgccaatccg acgtgtcgcg
     1621 gaccaggcgg acgagacgcg catgctgggt gaggttcttg atcaccacgt cgggattctg
     1681 ccgccggacg gcccccacga tggcctttcg ccaatccgcg tcccactcca cttccacatc
     1741 gacgggcacg ccaagcgacc gggtgtgcgt gacgatcctg ttcacccatt cgcgaacccg
     1801 ctcaagggtg gccgcttcga cgtgctcgaa ggtgtcggct gcaaccgcag tcaccgactt
     1861 gaagttgacg caacagtaaa cgtgaagatg ggtgtcctgg gaccggtgag cccggttgtc
     1921 cagagctatc ttctcgcccc attgaagcga cgcctgctcc atcgtcgtgg gatcgagcac
     1981 cacgaacact tttctcgtct ctgccatcga ctgctgactc cgcgttgatc tcgattggtc
     2041 aagtttcacc gcatcggcgc ggcgggcata tcggcacttc cccgtagacg ccacggttgt
     2101 ctagatggag cggatctgcg agatttcccc actggcccat cttccggacg atcgaatgcc
     2161 caatgccgat ccgtttgcgc cggagattcc cgagcccgtg ctcgaggacc tgcgacggcg
     2221 cctcacgact atccgatggc cccatgtcat cggcgaggac gactgggttt acggagttcc
     2281 ccaggcctgg ctgaagggca tggccgacta ctgggcccac gaatggaact ggaactcgac
     2341 cgctaccgcc atggcggcct tcgaccacta cctggccgac atggacggca tacccgttca
     2401 ctttcttcac gtcccgggca aaggcccggc gccaaagccg ctgatcctga ctcacggatg
     2461 gccctggacc ttctgggact acaaggatgt catcggcccg ctgagcgacc cggccgcgtt
     2521 tggcggcgat gcagctgacg ccttcgacgt ggtggtgccc tctttgcccg gtttcggatt
     2581 ttccaccccg ctcaccagtg ccggtatcga cgtcgccgag gtagcccggc tctgggttcg
     2641 gctcatgcag gatgtgtgcg ggtacgagcg cttcgccgcc cacggcggtg actggggcgc
     2701 cctggtgacg gctcatctgg gccatgcgca cgcgcagagc ctgatcggcg cgcacctggc
     2761 gctgccgctg attcccggcg tcgaccgagc ctccgtaccc agggaggcct acgcggagga
     2821 cgagcaatgg atgctcgaac gcgcagcgca atccggccct ctcattcgca gccacgtgag
     2881 cgtgcacacg acggatccgc aaaccctggc gtacgcgatg tccgactcgc ccatcggaac
     2941 ggcggcgtgg atctgggagc ggcgcagaaa ctggagcgac tgcgacggcg acgtggagcg
     3001 cgccttcgac cgcgatcacc tgtgcaccac cgcagcgctg tattggtgca cgggcgcttt
     3061 caccagctcg ctgcgactgt atttcgagca cttcaacaag ccctggccgc tggtgcacga
     3121 caggaagccc accgtcgaag cgcccacggg cttcgccata tttcccaagg acgtggtgca
     3181 gatgccacgg cgggttgccg ccgagcacac ggatctccgc cgctggagcg tcatgccgcg
     3241 aggcggacac ttcggagcgg cagaagctcc cgatctgatc gtcgatgaga ttcgcagctt
     3301 tttccgcgac ttgagctgac aggagcgccg agatcatggc caagcaccac gcacaagacc
     3361 tgagccgccc ctccgacccg aacctggcgc ccggcgcagc gcgctgcccc gggccgagct
     3421 ttcaggacat cgtcaggacc gaggacagcg ctaggtccac ggctctgacc acgcagtcct
     3481 atgattttct gggcgacgag gacgtgccct tctctcgcta tacgtccagg gaaatgtacg
     3541 agctggagat cgagcgtgtc tggagccgca catggcagtg ggcctgcaga gaggagcaca
     3601 ttccctcgcc cggtgattac cacgtctacg acgtggggcc ttactcggtt ctcgtgatcc
     3661 gcagcgatcc caacaccgtc cgcgcgtttg tcaattcctg tccccaccgc ggcatgcagt
     3721 tctgcgactc gggtacgtcc ggacggggca agcagtttct gcgctgcccg ttccacggca
     3781 tgtcctggca cctggacggt tcgctgcgag agattccctg ccgctgggac ttcccccacg
     3841 tcgaagagga aaatttcggt ctgacgcctg tcgcgtgcga gacctggggc ggtttcgtgt
     3901 tcatcagcct ggatcctgag ccggagccgt tagcgaccta tctcggcgtg ctgccggagc
     3961 acttcagcga gtggccgctc gaggagcgct acgtcgcgct gcacatgcag aaggttctgc
     4021 ccggcaactg gaagatgtgc atggaaggat tcatggaggc ctatcatgtc atggctacgc
     4081 atccggaggg cttgaagaca tctgcctggg ctacgacgca gtacgatgtc ttcgggcgcc
     4141 acgtgagccg cttcatgcag ccccgggcga tgccgagccc ctactacggg cggcccgtca
     4201 gcgagagaga ggcgtttgcc gcgctcggac acgacccgga agatttcccg gagggttcaa
     4261 gcgcccggca ggcgcacgcg gactcggttc gtcgcagcat gggcgagtct ctcggcgtcg
     4321 acctgacggg atacaccaac agccagctgc tcgactccat cgagtatcac ctgttcccca
     4381 acgcctgcct cttccccggc atcatcgtcc cgttcatcta ccgctttcgt ccgctcacgt
     4441 gggacagctg tctgcacgac atcctcgttc tgcatccggt gcccgatagc ggtccgcgtc
     4501 ccccacccgc cgagcccatt cgcctgggaa tcgacgactc gtacacatcg gttcccggat
     4561 tcagcgagaa tcgactggca ctcgtgctgg atcaggacac cgacaacttc cgccgccagt
     4621 gggcgggcat gcaggcctcc agaaagccgg gggaaaccct tgccaactac caggaggtgc
     4681 gcatccggca cttccaccag accctggacg cgtatctcgc ttctgcttag gcgtcgaaga
     4741 gaaggtccag tcgctgaaga ccccgggtgg cgtaggtggg cacgtactct agctcgcttc
     4801 cgggtttgag acggacgccg gacagccggt ctagcacggt ctcgaacgcg atcctggctt
     4861 ctgcgcgcgc cagcatcaat ccgacgcaga gatggggccc atagccgaag ccgacgtgct
     4921 ggttgacgtt ctcacgttcc agatcgaact gctcgggacg ctcgaagaag tccggatcgt
     4981 gtccggctga gccccagccg agcatgacgt tcgaccccgc tggtatcgtc acgcccccca
     5041 actccgtgtc gcgggtgacc acgcggtaaa ggcattgcac gggcgcatcg aaacgcatga
     5101 cctcttccag aaacggcggt atcagacccg cgtcctcgcg aagtcgcgcc atgacctcgg
     5161 gctgttccag cagtatccgc atgccgttgc cgatgaggtt cgccgtggtc tcgttaccgg
     5221 caagcagaag cgtggagatg atcggcagaa tctccgctac ctcgagccgc tctccgtcga
     5281 tctccgccgt ggccagcgag ctgatgaggt cgtcgcgcgg attccgccga cgggcctcca
     5341 ccgtatcggc aaaatatctg gtcgcttcca cccacgaagt tgccacctcc cgacgacgct
     5401 catgggatag gacatccaga ttgccggcca gaatatcgtc ggaccagtgc ctgaaggtgg
     5461 cgatgtcctc gcggggcaca ccgagcgcgt cagcaatgac gatgagcggg atcttgacgg
     5521 cgaactcgga gatgaagtcc atctcgcccc gcgactccac cgcgtcgatg aggtcgctgg
     5581 cgatttcccg gatcgcgcgc tcctgggcct ccacccagcg tcgggtga
//
LOCUS       DBNHQR010000173         3507 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1536175, whole genome shotgun sequence.
ACCESSION   DBNHQR010000173 DBNHQR010000000
VERSION     DBNHQR010000173.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1536175"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="AC16VR_03925"
     CDS             complement(<1..759)
                     /locus_tag="AC16VR_03925"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6010336.1"
                     /translation="MTVSAGLGIANFPFQDGPDFWRWVDLCDAGGVDSIWQSDRIISR
                     DPQLECMSVMAALAGGSRRLKFGMNVASLALRDPVLTAKACATIDVLSDGRLLPAFGV
                     GSALSRDFTATGTPTRGRGKRTAEGLEIMARLWSEESVSFHGEFYQLDDASISPRPVQ
                     QPLPLWVGGSAKQAIERTARWGTGWQAGIESAEEVAPVIAAIKAEAAQLGRSIDEDHF
                     GAGFGFRFGSPDEPICTRYLELLRKRLGREPKGFL"
     gene            854..1927
                     /locus_tag="AC16VR_03930"
     CDS             854..1927
                     /locus_tag="AC16VR_03930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6010337.1"
                     /translation="MEDRRYLVVGARGLVGRAVLSALEAEDRAEVIGLSRRQPDFPTR
                     ARWIPCDLTDRDACHAALGAAQTQGVTHIVYAALQEMPDLISGWRDPVQIETNARMLA
                     NLLEFVQPREHFTLLQGTKAYGAHLAPMALPGKESAPRHPGENFYWEQEDLMRSRQAG
                     SSWAFSIVRPQIVCGVALGSPMNMVAAIGVYAAVMRQLGKPLCFPGGGGFVTEATDAD
                     LLARAIIWAGREPACANEILNVTNGDVLDWVSLWPAFGEIFQMPTGEADPMRLAISMP
                     ELSGVWDEVVGRHGLKSMAMAELVGDSWQFADAVFGYGGGARSTLLSTIKIRQLGFHE
                     CIDTEQMFRNQLAALQDLKVLPR"
     gene            1927..2874
                     /gene="glk"
                     /locus_tag="AC16VR_03935"
     CDS             1927..2874
                     /gene="glk"
                     /locus_tag="AC16VR_03935"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HIR6010338.1"
                     /translation="MADGAYLVADIGGTNARLALTDGTRLTSPVVISQTASLRNVAGL
                     AEAARSAFDGQALDGCCIAMAGPVHANQGSLTNVSLAVSGDALGAELGCPARVVNDFY
                     AVARGIPVLRHLRQIGPGEPGEGVRALLGPGSGLGMAVLAPTREGPPLVLPSEGGHAD
                     LAPGSPLEQEVLQVLAARLPHVSWESVLSGPGLVNLYCALCAIWGVPEEALAPEEISR
                     QGLVVEDPICHQVLEMFFGFLGAAAGNLALTVCAEGGVFIGGGIVPKLVEFAESSPLR
                     RRFEERGVLADYAARLPLYVIEDEFPGLLGAVACLADQP"
     gene            complement(2865..>3507)
                     /locus_tag="AC16VR_03940"
     CDS             complement(2865..>3507)
                     /locus_tag="AC16VR_03940"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6010339.1"
                     /translation="QLRVARSTARLRGLDNHVRFELQDYRQVEESFDRIVSVGMFEHV
                     GRRHYDEFFGKVEALLADDGVALLHTIGASSPPSVVNPWIERHIFPGGYIPSLSDVAP
                     AVERSGLITADVEVLRHHYALTLQEWNRRFQQRRAEFAQSKGEAFCRMWEFYLLACQT
                     AFEVSDLVVHHWQLAKRNLSVPTTRNYLYETQPYVKIPGETAAEEITLRQHQG"
BASE COUNT          547 a         1150 c         1203 g          607 t
ORIGIN      
        1 gagaaagccc ttgggttcgc gtcccaggcg cttgcgcagc agctccagat accgcgtgca
       61 gatcggctcg tccggtgagc cgaaacgaaa accgaaaccc gcgccgaagt gatcctcgtc
      121 gatggaccgg ccgagctgcg ccgcctctgc cttgatggcg gcgatgacgg gcgctacctc
      181 ctccgcggac tcgataccgg cctgccagcc ggtgccccag cgggcggttc gttcaatggc
      241 ctgtttcgcg gagcccccca cccagagcgg cagcggctgc tgaacgggcc ggggcgagat
      301 gctggcgtcg tccagctggt agaactcccc atggaagctc acgctttcct cggaccatag
      361 cctggccatg atttccagac cctccgccgt gcgtttcccg cgaccgcggg tgggtgtgcc
      421 cgtggcggtg aaatctctgg acagagcgct gcccacgccg aacgcgggga ggagccgccc
      481 gtcgctgagc acgtcgatgg tggcgcaggc cttggcggtg agcacggggt cgcgcagcgc
      541 caggctcgct acgttcatgc cgaacttcag gcgccggctg ccgccggcca gcgcggccat
      601 gacgctcatg cattccagct gcgggtcgcg actgatgatc cgatcgctct gccagattga
      661 atccaccccc ccggcgtcgc acagatctac ccagcgccag aagtctggcc cgtcctggaa
      721 cggaaaattc gcgatgccga gtccggcgct gaccgtcatg aatcaatcct ggcttcgggg
      781 gaaccggatt atagcgggcc gagttggtgg cctggcaatt gaaccggatg ggtggcatgc
      841 ggacaatggc ggcatggagg atcgcagata tctggtcgtg ggggcccggg ggctcgtcgg
      901 ccgcgccgtg ctgtcggctc tggaggctga agatcgagca gaggtcatcg ggctttcccg
      961 tcggcagccg gactttccca cccgggcgcg ctggatcccc tgcgatctga cggaccgcga
     1021 tgcgtgccac gcggcgttgg gcgccgcgca gacgcaaggc gttacccaca tcgtctacgc
     1081 cgctcttcag gaaatgccgg atctgatatc gggctggcgg gaccccgtcc agatcgaaac
     1141 caacgcgcgg atgctcgcca acctgctgga gttcgtgcag ccacgggagc acttcacgct
     1201 gctgcagggg accaaagcct acggcgctca tctcgcgccc atggcgttgc cggggaagga
     1261 gtcggcgccc cggcacccgg gcgagaactt ctactgggag caggaagacc tgatgcgcag
     1321 ccgccaggcc ggatcgtcct gggccttcag catcgtccgc ccacagatcg tctgcggcgt
     1381 agcgctcggc agccccatga acatggtggc ggccatcggt gtttacgccg ccgtgatgcg
     1441 ccagctcggc aagccgcttt gctttccggg tggcggcggt ttcgttaccg aggccacgga
     1501 cgccgacctg ctggcccgcg cgatcatctg ggccgggcgc gaacccgcct gcgccaacga
     1561 gatcctcaac gtcaccaacg gcgacgtgct cgattgggtc tccctgtggc ccgcgttcgg
     1621 tgagattttt caaatgccca ccggcgaagc ggaccccatg cggctcgcga tcagcatgcc
     1681 ggaactctcc ggtgtctggg acgaggtggt cggacggcac gggcttaagt ccatggccat
     1741 ggccgagctg gtgggcgatt catggcagtt cgccgacgcg gtattcggct atggcggcgg
     1801 ggcgcggagc acgctgctga gcacaatcaa gattcgccag ctcggcttcc acgaatgcat
     1861 agacaccgag cagatgttcc gcaaccagct tgccgccctg caggatctca aggtgctgcc
     1921 tcgctgatgg cggacggagc gtatctggtc gcggacatcg gtggcaccaa tgcccgcctg
     1981 gcgttaaccg acggaacgcg gctcacgtcc cccgtggtga tttcgcagac ggcctcgctc
     2041 aggaacgtgg ccggtctcgc cgaagccgcg cgttcggctt tcgacggcca ggcgttggac
     2101 gggtgctgca tcgccatggc gggccccgtt cacgccaatc agggcagcct gacgaacgtg
     2161 tcgctggccg tttcggggga cgcgctgggt gccgagctcg gctgtcccgc ccgggtggtc
     2221 aacgatttct acgccgtggc tcggggcatc ccggtcctgc gccacctgcg tcagatcgga
     2281 ccgggcgagc ccggcgaggg cgtcagggcg ctgcttggac ccggcagcgg gttggggatg
     2341 gccgtgctgg cgccgacccg ggaaggtccg cccctggtgc ttccgtccga gggcgggcac
     2401 gccgacctcg cgcccggctc tcccctggag caggaggtgc tgcaggtgct tgccgcaagg
     2461 ctcccccacg tcagctggga gagcgtgctg tcaggacccg gcctggtgaa cctctactgc
     2521 gcgctatgcg ccatctgggg agtgccggag gaagcgctgg ctcccgagga gatcagcagg
     2581 caggggctgg tcgtggagga cccgatctgc catcaggtgc tggaaatgtt cttcggcttt
     2641 ctcggcgccg cggcggggaa cctcgcgctc accgtctgtg cggaaggcgg cgtattcatc
     2701 ggcggcggca tcgtgcccaa gctcgtcgag ttcgcggagt ccagccccct gcgcaggcgc
     2761 ttcgaggaac gcggcgtgtt ggccgactac gcagcgcggc tgccgctcta cgtcatcgag
     2821 gatgagtttc cgggtctgct tggcgccgtt gcctgcctgg ccgatcagcc ctgatgctgc
     2881 ctgagggtga tctcttcggc cgcggtttcc ccggggatct tcacgtacgg ctgcgtctcg
     2941 tacagatagt tgcgcgtggt gggcacgctc aagttgcgct tggccagctg ccagtggtga
     3001 accaccagat ccgatacctc gaaggcggtc tggcacgcga gaaggtaaaa ctcccacatg
     3061 cggcagaacg cctcgccttt gctctgagcg aactcggcgc gcctttgctg gaaccggcgg
     3121 ttccactcct ggagcgtcag cgcgtagtga tgacgaagga cctccacgtc ggcggtgatc
     3181 agcccgctgc gctccacggc tggcgccacg tcggagagtg aagggatgta gccgccggga
     3241 aaaatgtggc gctcgatcca cgggttcacc acgctgggcg ggctggacgc cccgatggtg
     3301 tgcagcagcg caacgccgtc gtctgcgagc agcgcttcga ccttgccgaa gaactcgtcg
     3361 tagtggcgac ggccgacgtg ctcgaacatg ccgacggaga cgatcctgtc aaagctctcc
     3421 tctacctgcc ggtaatcctg aagctcgaag cgcacgtgat tgtcgaggcc ccgcaggcgg
     3481 gccgtgctcc gggccacacg cagctgc
//
LOCUS       DBNHQR010000174         1752 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_523352, whole genome shotgun sequence.
ACCESSION   DBNHQR010000174 DBNHQR010000000
VERSION     DBNHQR010000174.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_523352"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="AC16VR_03945"
     CDS             complement(<1..265)
                     /locus_tag="AC16VR_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010340.1"
                     /translation="MAEAVKDLDPDNMVRSPGLTYQQLLDQDTHPVPDVLRLQSPKDF
                     GDADISIERYISKEWHDREVERLWKRVWQFACREEDIPDPGDHY"
     gene            complement(360..1742)
                     /locus_tag="AC16VR_03950"
     CDS             complement(360..1742)
                     /locus_tag="AC16VR_03950"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6010341.1"
                     /translation="MHATSGGIENVERILRTYPEVTDISAFIGKGPSRFTSIVRPEQP
                     NPAYAHLVVRITDVNLMDAVIRRARQAVAEQIFDADIQIRRGEFTSSGTSKIEARFSG
                     PDPDVLRHLAEQALEVYLAHDLVERKTDWRQPELQIVPRFDEDRARVAGVSRTDVSRA
                     LALATLGVEIGLFRDGNKLLPIIARAPHEERLDVGALSDRLVWSPAQQAHIPMRQIVS
                     SFELVAENNTIYRRDRERTIQARANPPPGHNPTMVFNSIRPGVEAIELPPGYILEWGG
                     EHESSEDAKRLLSSKIPLTFGLMLFISILMFGKLRQPLVIWLTVPMTVCGVVVSLLVT
                     DLTFTFTAFLGFLSLSGMLIKNCIVLVDEIDKRMLEGAHSLTTIVEASVSRLRPVMLA
                     AGTTIAGMSPLLADAFFREMAVCIMGGLTFATLLTLVAVPVFYRIAFRRQLSSAQQAS
                     PGVLAGQAGG"
BASE COUNT          340 a          551 c          535 g          326 t
ORIGIN      
        1 gatagtgatc gccggggtcg ggaatgtctt cctcccgaca agcgaactgc cacacgcgct
       61 tccacagccg ctccacctca cggtcatgcc actctttgga gatatatcgc tcgattgaga
      121 tgtcggcatc gccgaaatcc ttcggcgact gcagcctcag cacgtcggga accgggtggg
      181 tatcctgatc gagcagctgc tgatatgtga ggcccggcga gcggaccatg ttgtccggat
      241 ccagatcttt gacggcttcg gccattgcgt ttccccaaga agagtaagcg atcgggcgat
      301 gcgcatcgcc ctcgcgctac agattcgccg tgctcaccac gagtgtcaag gcgctcccgt
      361 cagcctcctg cctggccggc caggacaccc ggagaggctt gctgcgcgga ggaaagctga
      421 cgcctgaaag cgatgcggta gaacaccggc acggccacca acgtcaacaa cgtagcaaac
      481 gtaagccccc ccatgatgca gaccgccatc tcccggaaga aggcgtcggc cagcaacggc
      541 gacatgcccg ctatagtggt cccggcggcg agcatcaccg gccgcagccg gctcacgctg
      601 gcctcgacga tggtcgtcag gctatgggcg ccctccagca tccgcttgtc gatctcgtcc
      661 acgaggacga tgcagttctt gatcagcatg ccggacagac tcagaaaacc gaggaacgcc
      721 gtgaacgtga aggtgagatc ggtaaccagc aggctgacga cgaccccgca gacggtcatg
      781 gggacggtaa gccagatcac caggggctgt cgaagcttgc cgaacatgag aatgctgatg
      841 aacagcatga ggccgaaggt gagcggaatc ttgctggaca gcagtcgctt cgcgtcctcg
      901 ctcgattcgt gctcaccgcc ccactccagg atatatccgg gcggcagctc gatggcttca
      961 acgccgggcc gtatggagtt gaacaccatg gtggggttgt gtcccggcgg cgggttcgcc
     1021 cgcgcctgga tcgttcgctc acggtcgcgt cggtaaatcg tgttgttctc cgccaccagc
     1081 tcgaagctgg agacgatctg gcgcatgggt atgtgggctt gctgcgcggg gctccacacg
     1141 agacggtccg acagcgctcc aacgtccagc ctctcttcgt ggggcgcccg ggcaatgatg
     1201 ggcagcagct tgtttccgtc ccggaagagg ccgatctcga cgcccagcgt ggccagcgcg
     1261 agcgcgcggg aaacgtccgt ccgggagacg cccgccaccc gcgcgcgatc ctcgtcgaag
     1321 cgcggaacga tctgcagctc cggttgccgc cagtccgtct tccgttcaac caggtcgtgg
     1381 gcgaggtata cctccagcgc ctgctctgcc aaatgcctga gcacgtccgg atccgggccg
     1441 gagaaccgcg cttcgatctt cgaggtcccg ctggaggtga actcgccgcg ccgtatctgg
     1501 atgtcggcat cgaaaatctg ttctgccacc gcctggcggg cacgtcgaat caccgcgtcc
     1561 atcaagttga cgtcggtgat ccgcaccacc agatgagcgt acgccggatt tggttgctca
     1621 gggcgcacaa tcgacgtaaa gcgggatggt cccttaccta taaaagctga tatatcagta
     1681 acttctggat atgttctaag tatcctttcg acgttttcaa taccgccgga agtggcatga
     1741 atgtccgttc cc
//
LOCUS       DBNHQR010000175         4139 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_902983, whole genome shotgun sequence.
ACCESSION   DBNHQR010000175 DBNHQR010000000
VERSION     DBNHQR010000175.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4139)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_902983"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..1395
                     /locus_tag="AC16VR_03955"
     CDS             133..1395
                     /locus_tag="AC16VR_03955"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HIR6010342.1"
                     /translation="MTDIAAYMGEVARSARAASRVIAAASSDSKSRALKAMVEALDED
                     RETLKRANEVDVRAAGDKGVDQPLIDRLVLDDRAIDRMLEGVLQVDKLADPVGEITDL
                     AYRPTGIQVGRMRVPLGVIGIIYESRPNVTADAASLCLKSGNACILRGGSEAIESNRA
                     IAAGIARGLAAAGLPETVVQLVDTTDRDAVGELLKADRFVDVIVPRGGKGLIERIIRE
                     SRIPVIKHLDGVCHVYIDDAADLEMAAAIAVNSKIEKYAVCNAMETLLVANGVADRVL
                     PVLGDRFRQAGVELRGCERARAAFSDMKPATEEDWREEYLGPVLAVRVVEGIDEAIAH
                     INTYGSQHTDAIVSNDYGRTRRFLAEVDSASVMVNASTQFADGFEFGLGAEIGISTDK
                     LHARGPVGLEGLTSQKFVVFGGGEIRHR"
     gene            1392..2024
                     /gene="nadD"
                     /locus_tag="AC16VR_03960"
     CDS             1392..2024
                     /gene="nadD"
                     /locus_tag="AC16VR_03960"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HIR6010343.1"
                     /translation="MIALFGGTFDPVHRGHLHGAATVCEALGLEAISMVLSARPGHRA
                     SPGASAEHRWEMLCLACADDGRLVPDDRELHRDRPSYTVETLLEVRGEIGDESLVWVL
                     GSDAYADLTSWHRWGEVLDLTNLVVLERPGGPSRWAPPLDGISRRHRVEGMLDAPWGQ
                     ILFLKAPMQEVSASEIRAAIAGGRPVAHLLPERVATYISRHGLYGAPSDS"
     gene            2014..2361
                     /gene="rsfS"
                     /locus_tag="AC16VR_03965"
     CDS             2014..2361
                     /gene="rsfS"
                     /locus_tag="AC16VR_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008176434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HIR6010344.1"
                     /translation="MTAEALRDLVVDALEERKGRDVVAMDVTKSTDITDFMIIASGTS
                     NRHVKALVDQVVESAKAGGNPPLGVEGRETHEWVLVDLGDVLVHVMQADTRAFYDLER
                     LWSDIPLDSETLN"
     gene            2381..2848
                     /gene="rlmH"
                     /locus_tag="AC16VR_03970"
     CDS             2381..2848
                     /gene="rlmH"
                     /locus_tag="AC16VR_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008176432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HIR6010345.1"
                     /translation="MKIRVIAVGARPRPWVADGFREYARRLPREMPLSLVEVAPGGGR
                     GRSAEQVVAMESERLLSHLGPRDWVVALDVGGEGCSTRELALSLESWRMQGRDVAFLI
                     GGAEGLAPTCLARADMALSLSELTFPHEMVRVILAEQIYRAWTILSGHPYHRD"
     gene            2850..>4139
                     /gene="mrdA"
                     /locus_tag="AC16VR_03975"
     CDS             2850..>4139
                     /gene="mrdA"
                     /locus_tag="AC16VR_03975"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HIR6010346.1"
                     /translation="MPPVLALKDSSSERRSFSARAAVLFALATCLVSVLILRLLQLQV
                     WEYDTYRTRSEENRIQVQPLAPPRGLIFDRNGVLLADNRPVHSLALVRERIADLDALI
                     TELGSLVSISEAEIESLRGRLLRRRRPYEPVALKLTLSEEDIAVLAVNRHRLVGVEIS
                     TQLVRYYPFGPLLAHAVGSVRRVTEDDLRELDPVRYSATKFVGRRGVERFYERSLHGE
                     VGYQRVETDAHGRIRQVLEINEPAAGQNITLQLDSRLQIAASAALGDRRGAIVALDPR
                     SGGVLALVSHPQYDPNQFVTGMTSAQFRELTSSRDTPLFNRAINGQYAPGSTFKPIVG
                     LAGVASGVTRWADQIDDQGWYQLPGQERIYRDWSWAVDDSGGQGLTNLTRAIYRSSNV
                     YFYDLATRLDIDRIATFAAQFGYGQRTAVDIPDASKGL"
BASE COUNT          696 a         1291 c         1411 g          741 t
ORIGIN      
        1 ggatgctgcc ctacgtcgtc tatcggctga tgctgggcac cttgctgctc ggtctcgccc
       61 tgtgatcgtc gcgcgctgac ctccggtata cttgccggtc cctaatctct gggccaaatg
      121 cccccgaaag tcatgacaga cattgccgct tacatgggcg aggtcgccag gagcgctcgc
      181 gctgcctcca gagtgatcgc tgcggcctcc agcgacagca agtcaagggc gctcaaagcc
      241 atggtggagg ctctcgatga agatcgggaa acgctgaagc gggctaacga ggtcgacgtg
      301 cgcgccgcgg gagacaaggg cgtcgatcag cccctcatcg atcgtctggt tctcgacgat
      361 cgtgccatcg accgaatgct ggaaggcgtc ctccaggtgg acaagctggc cgatccggtg
      421 ggggagatca ccgatctggc ctaccgtccg acgggcattc aggtggggcg gatgcgcgtg
      481 cccctgggtg tgatcgggat catctacgaa tcgcgcccga acgtgaccgc ggacgctgcc
      541 agcctgtgtt tgaagtcggg caacgcctgc atccttcggg gcggttcgga agccatcgag
      601 tcgaaccgag ccatcgccgc gggaattgcc cgtggcctgg ccgcggcagg actgccggag
      661 acggtcgtcc agctcgtcga cacgacggat cgcgatgcgg tgggagagct gctcaaggcg
      721 gatcggttcg tggacgtcat cgtgcctcgg ggaggcaagg gcctgatcga gcgcatcatc
      781 cgggaatcac gaatcccggt catcaagcac ctcgacggcg tgtgccacgt ctacatcgac
      841 gacgccgctg acctggagat ggcggccgcc atcgccgtca actccaagat cgagaagtac
      901 gcggtctgca acgccatgga gacgctgctg gtggccaacg gcgtagccga tcgggtcctg
      961 cccgtgctcg gcgaccgttt tcgccaggcg ggcgtggaat tgcgaggctg cgagcgggct
     1021 cgggccgcct tttcggacat gaaaccggca accgaggaag actggcggga ggagtatctc
     1081 gggccggtgc tggcggtgag ggtggtggag gggatcgacg aggcgatcgc tcacatcaac
     1141 acctacgggt cccagcatac ggacgccatc gtctccaacg actatggcag aacccgtcga
     1201 tttcttgcgg aggtggactc ggcgtcggtc atggtcaacg cctcgaccca gtttgcagat
     1261 ggcttcgaat tcggcctggg agccgagatc ggcatctcga cggacaagct ccatgcccgt
     1321 ggcccggtgg gcctcgaagg cctcacgtcg cagaagttcg tggtcttcgg cggcggagag
     1381 atccggcacc gttgattgct ctgtttggcg gcaccttcga tccggttcac cggggacatc
     1441 tgcacggcgc ggccacggtc tgcgaggcgc tcggcctcga agccatctcc atggtgctgt
     1501 ccgcccggcc gggtcaccgc gcttcgccgg gagcgtccgc cgagcaccgc tgggagatgt
     1561 tgtgcctggc gtgcgccgac gatgggcgcc tggttccgga tgatcgcgag cttcatcgtg
     1621 accggccgtc ctacacggtg gagacgctgc tggaggtccg cggagaaatc ggtgacgagt
     1681 ccctggtctg ggtgctcggc agcgacgcct acgcagatct gaccagctgg catcgctggg
     1741 gtgaagttct ggatctgacc aacctcgtgg tgctggagcg gccgggcggg ccgagccgct
     1801 gggccccgcc cctggatgga atcagccgtc gtcaccgggt cgaggggatg ctagacgcgc
     1861 cgtgggggca gattctcttc ctgaaagctc ccatgcagga ggtgtccgcc tcggaaattc
     1921 gcgcagccat cgccggcggg cgccccgtgg cgcatttgct cccggagcga gtggcaacat
     1981 atattagccg gcacggacta tacggagcac cgagtgacag ctgaggccct gcgcgatctg
     2041 gtcgtggatg cgttggaaga acggaaaggc cgcgacgtcg tggcgatgga cgtgaccaag
     2101 agtaccgaca tcacggattt catgatcatc gccagcggca cctccaatcg gcatgtgaag
     2161 gcacttgtcg atcaggtggt ggagtcggcg aaagccggcg gtaacccgcc gctcggcgtg
     2221 gagggccgtg aaacccacga gtgggtgctg gtggatctcg gtgacgtgct cgtgcacgtg
     2281 atgcaggccg acactcgggc attctacgac ctcgagcggc tctggtccga cataccgttg
     2341 gactccgaaa cgctcaactg aggcgcccgg gaaggctgcc atgaagatcc gggtcatcgc
     2401 cgtgggcgca cgacctcggc cctgggttgc cgacgggttt cgcgagtacg ccaggcggtt
     2461 gccgcgggaa atgccgctgt ctcttgtgga ggtggcgccc ggcgggggcc gtggccggtc
     2521 tgccgagcag gtcgtggcga tggaatcgga acgattactc agccacctgg gtccaagaga
     2581 ctgggtcgtc gccctggatg taggaggtga gggttgcagc acccgggaac tggcgctgag
     2641 cctggagagc tggcggatgc aggggcggga cgtcgctttc ctcattgggg gcgccgaagg
     2701 gctcgcgccc acgtgcctgg ctcgtgctga catggcgctg tcgctgtccg agctgacttt
     2761 cccccacgag atggtgaggg taatcctggc ggagcagatc taccgggcct ggaccattct
     2821 ctccggccac ccctatcatc gggactgaca tgccgcctgt tctcgcactc aaagacagct
     2881 cgtccgaacg ccggtccttc tctgcccggg ccgcggtgct gttcgcgctc gctacctgcc
     2941 tcgtatccgt cctgatactc cgtctgctgc agctgcaggt gtgggagtac gacacgtacc
     3001 ggacccggtc ggaagagaat cgcatacagg ttcagccgct ggcgcctcca cgagggctaa
     3061 tttttgatcg caacggcgtg ctgctggccg acaatcgtcc cgttcacagc cttgctctgg
     3121 tgcgcgaacg aatagccgac ctcgatgccc tcatcaccga gctgggctcg ctggtcagca
     3181 tcagcgaggc ggagatcgaa agcctccgcg gccgcctgct gcgacgccga cggccctacg
     3241 agccggtggc gctcaagctg acgctcagcg aggaagacat cgccgtgctt gccgtaaacc
     3301 gccaccggct cgtcggcgtt gagatctcca cccaactcgt ccgctactac ccctttggtc
     3361 cgcttcttgc gcacgctgtg ggctcggttc gacgagtcac cgaggacgat ctgcgcgagc
     3421 tagaccccgt tcgctacagc gccactaaat ttgtcgggcg cagaggggtg gagcgctttt
     3481 acgaacgctc gctgcacggc gaggtgggct atcagagggt ggagaccgat gcccacggcc
     3541 ggatccgcca ggtgctggag atcaatgagc ctgcggccgg tcagaacatc acgcttcagc
     3601 tggacagccg cctgcagatt gccgcgtcgg ctgccctggg agatcggcgc ggcgccatcg
     3661 tggccttgga cccccgcagc ggtggcgtgc tggccctggt gagtcacccg cagtacgacc
     3721 ccaaccagtt cgtgaccggc atgacgtcgg cccagtttcg ggagctgacg tcctctcggg
     3781 acaccccgct gttcaatcgt gcgatcaacg gccagtacgc gccgggctcg accttcaaac
     3841 ccattgtggg gctcgcgggg gtcgcgagcg gcgtgacgag gtgggcggat cagatcgacg
     3901 atcaggggtg gtaccagctg cccgggcagg agcgcatata ccgggactgg agctgggcgg
     3961 tagacgactc gggaggtcag ggcctcacca acctgacgcg agccatttac cggtcttcca
     4021 acgtctactt ctacgatctt gccacccggc tggacatcga ccggatcgcc accttcgccg
     4081 cccagttcgg ctacgggcag cgcaccgcgg tcgacattcc cgatgcgagc aagggcctg
//
LOCUS       DBNHQR010000176         6740 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_207161, whole genome shotgun sequence.
ACCESSION   DBNHQR010000176 DBNHQR010000000
VERSION     DBNHQR010000176.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6740)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6740)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6740
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_207161"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="AC16VR_03980"
     CDS             complement(<1..407)
                     /locus_tag="AC16VR_03980"
                     /inference="COORDINATES: protein motif:HMM:NF014790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6010347.1"
                     /translation="MSDLEAFRLETREWLEANCPPGVRNRPGEVVWGGRRCQFPNEDA
                     KIWLERMVEKGWTVPTWPKPYGGAGLTDEEAKVLRQEMARIEANAPLFSFGIAMLAPA
                     LMEFASEEQQLEHLPPIARGDFRWCQGYSEPGAG"
     gene            complement(490..1347)
                     /locus_tag="AC16VR_03985"
     CDS             complement(490..1347)
                     /locus_tag="AC16VR_03985"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6010348.1"
                     /translation="MKIGAVFPHLEIGSDPAVLRDWAQTAEDLGYSHLLAYDHVLGAV
                     HAGREPALWGPYTEESAFHEPFVLFGYFAAVTRRVELVTGVIILPQRQTALVAKQAAE
                     IDILSGGRLRLGVGTGWNYVEYDSLGENFANRGKRQEEQIEVLRSLWAEPVIDYSGNY
                     HRIERAGLKPLPGRRIPIWLGGFSDVAYRRAARMGDGFIFGGSIKDGLAGLGKIRGFL
                     AEAGREQAGFGIEAMVNYGAGPDRWRADLEAWEAAGADYVSMRGMDAGLETPQDHIDA
                     LRRYWEAVS"
     gene            complement(1380..2156)
                     /locus_tag="AC16VR_03990"
     CDS             complement(1380..2156)
                     /locus_tag="AC16VR_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019408213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6010349.1"
                     /translation="MTPELVKTELRPDGIAVVTIDNPPVNAHSGQVLTEMAWTFDAIS
                     DQDEARVVVLTGAGKVFCAGADIKSRSGRAPEPGDHWQSSRAAREAYHSVRECVKPVI
                     GALNGPALGGGLAFAASCDMLIAAETACLGLPEIDVGLLGGGRHAQRLFGHSRLRRMM
                     LTGYRVYGPELYRLGIVEACVAPDALMDEALALAAEVAAKSPIAIRLAKHALNTIEEL
                     SLRDGYRFEQTMTGQLAKTEDSKEAMRAFVEKRKPQFKGR"
     gene            2288..3664
                     /locus_tag="AC16VR_03995"
     CDS             2288..3664
                     /locus_tag="AC16VR_03995"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6010350.1"
                     /translation="MSKSQPNQLLLTNARLFPGVSDEVFADGAVWIEGRTIRYAGPGC
                     DLPAVADDVPRVDLHGQFVMPGMTESHSHLSYSNNGPTELDKSPVEDAMIHAVANARL
                     MLGSGFTSAISFGSVHRIDVFLRDAIDAGRIPGPRLEASSRDVGATSSNADLHPDYFK
                     PQMEGLGMLADGPWAVRKAVRTIRKNRGNVVKLFLDGEGPGGHAPPGELTYTDEEVAA
                     AVDEAHRRGMRVACHSRSAAAVKQAVRFGIDFIGHANYLDDEALDMLKRSRERLFVGP
                     AIAWELAFLRHAAELGSPPGSQRYKFYEQEVEATKNAVRRLREAGVRVLIGGDYGLSI
                     TPHGTYAKDLQYFVELFGLSPAEALLCATRDGGAAVDPSGMLGTLEEGKLADLVIVDG
                     DPLEDITVVQDHDRITAVMKDGVVHRRLAVDNPYQASPDALQEELAARAADPERQRQA
                     AVLLESSG"
     gene            3708..4949
                     /locus_tag="AC16VR_04000"
     CDS             3708..4949
                     /locus_tag="AC16VR_04000"
                     /inference="COORDINATES: protein motif:HMM:NF014325.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HIR6010351.1"
                     /translation="MNLQTLATPRIGWRWPAALILFLLGFAGFATGVAPSERPDVAQA
                     DLLTKAYYSLGLFVVGGLDIGTPASGPLLGRAMLWIAYFGAPLLTASAVIEALVRVMA
                     PHRWQMRRLHDHVVIVGAGELTISYLRVLRGSHPSTPVVVVDEHVDPVREQELAQAFD
                     VRVVIGNITHDFVQNGLRLDRAARVVLLGDSDFQSFEAASKMLRRFPHLESRLVLHCQ
                     RLRFLRSMEATTVGRLCITFNSYHLAAASLVKQQLIGHFAKTEHRDVVVLAGFGRFGQ
                     TILEELQNLARDELDTVAIIDVDADRRVMVAEEQRRLGGNYRRVVLQGDISHPEVWRS
                     LAASVDLSKDSPTVILGTGRAEDNLRTALWLKQSYPNAFVFARTNDKSELAVEVGSEH
                     GIRNISIRQLVEDNIPEEWLA"
     gene            complement(4959..5438)
                     /locus_tag="AC16VR_04005"
     CDS             complement(4959..5438)
                     /locus_tag="AC16VR_04005"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6010352.1"
                     /translation="MERTVKISQGRHAIEDIMYGYAEGIDTGNLETVAPLFAHGVMVM
                     PDGSELNGPEEVLRTFSDVIIFYDDEENVVPYQRGACSPRTKHVVTNSIFEFDNAVRN
                     ADVRSYFTVYQSLGGANEIIVGGRYIDQYELMIQGWVLVRREVYLENVGDLSRHLNG"
     gene            5570..6670
                     /locus_tag="AC16VR_04010"
     CDS             5570..6670
                     /locus_tag="AC16VR_04010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6010353.1"
                     /translation="MAKRLIVMAGLLALVFGSLFGWKAFERHKTAEYLANARPPAVVV
                     SSTRVEEDRWRQQVESVGSLSAIQGVDVSSEVPGTVADISFQSGQQISAGELLVQLDA
                     TAERAELRSLEAQLVLARLDYERALGLLERTMLSQAQLDRAKSVMDSLQAQVEEQAAF
                     IAKKAIRAPFAGELGIRQVNLGEYVSPGTPIVTLQALDPIYANFALPERFLRELAVDQ
                     PLRVVVAAYPEDEFLGTVTAISPKVEETTRNVVVQATIANADRRLRPGMFARITVQTG
                     GDHRVLTVPRTAITFNPYGESVFLINEQDGALLVDRRQVITGEIRNERVEVVSGLLAD
                     DLVVATGQLKLRAGQRVKIDNSIDLSRPVTGP"
BASE COUNT         1138 a         2308 c         2161 g         1133 t
ORIGIN      
        1 ccggcccccg gctcgctgta gccctgacac cagcggaaat cgccgcgggc gatgggcggc
       61 aggtgctcga gctgctgctc ttcagaggcg aactccatca gcgccggggc gagcatggcg
      121 atgccgaacg agaacagcgg cgcgttggct tcgatgcgcg ccatctcctg gcgcagaact
      181 ttcgcctcct cgtcggtcag gccggccccg ccataaggct tgggccaggt gggcacggtc
      241 cagcccttct cgaccatgcg ctcgagccag atttttgcat cctcgttcgg aaactggcag
      301 cggcgcccgc cccagaccac ctctccgggc cggttgcgga cacccggcgg gcaattggcc
      361 tcgagccact cacgggtctc gaggcggaaa gcttccagat cgctcattgc atcccttttc
      421 ccagacagca accgctcatt ctagcctgtc caaagggctc ggtcatgacg caatcgcccg
      481 tccggcaact cacgatacgg cttcccagta acgtctgagc gcatcgatgt gatcctgcgg
      541 cgtctcgagt ccggcatcca tgccacgcat ggagacgtag tccgccccgg ctgcctccca
      601 ggcctccagg tccgcgcgcc agcggtcggg ccccgcgccg taattgacca tcgcctcgat
      661 gccgaagccg gcctgctccc tgcccgcctc ggcgaggaag ccccggatct tcccgaggcc
      721 ggcaagccca tccttgatgc tgccgccgaa gatgaacccg tcacccatcc gtgcggcgcg
      781 gcggtaggcg acgtccgaga acccgcccag ccagatgggt atccggcggc cgggcagcgg
      841 cttcaggccc gcgcgctcga tgcgatgata gttgcccgag taatcgatca ccggctccgc
      901 ccagaggctt cgcagcacct cgatctgctc ttcctgacgc ttgccccgat tggcgaagtt
      961 ctccccgagg ctgtcgtact ccacgtagtt ccagccggtg cccacgccga gcctgagccg
     1021 cccgccggag aggatgtcga tctccgccgc ctgcttggcc accagcgccg tctgccgctg
     1081 gggcaggatg atgaccccgg tgaccagctc gacccggcgg gtcactgccg caaaatatcc
     1141 gaagagcacg aacggctcat ggaaagccga ctcttccgtg taaggccccc acagcgcggg
     1201 ctcacgcccg gcgtgcaccg cgcccagcac gtgatcgtag gccagaaggt ggctgtagcc
     1261 gagatcctcg gcggtctggg cccagtcgcg aaggaccgcc ggatcgctgc cgatctccag
     1321 atgcggaaag accgccccta tcttcatttc attctccctg tatcagacga gccgagcctt
     1381 cagcggcctt tgaactgagg tttgcgtttc tccacgaatg cccgcatggc ttccttcgaa
     1441 tcctcggtct tagccagctg tccggtcatg gtctgctcga agcggtaacc gtcgcgaagg
     1501 ctcagctcct cgatggtgtt gagcgcgtgc ttggcaagcc ggatggcgat ggggctcttg
     1561 gccgcgacct cggccgcgag ggcgagcgcc tcgtccatga gcgcatccgg ggccacgcag
     1621 gcctccacga tgccgagccg gtagagctcc ggaccgtata cccgatatcc ggtgagcatc
     1681 atacgccgca gccgggagtg gccgaacagc cgctgggcat gacgcccgcc accgagcagg
     1741 cccacgtcga tctcgggcag gccaagacag gcggtctcgg cagcgatcag catgtcgcag
     1801 gaagcggcaa aggccaggcc gccgccaagg gccggcccgt tgagcgcccc gatcacgggc
     1861 ttcacgcatt cgcgcacgct gtggtaagcc tcccgggccg cccggctgct ctgccagtga
     1921 tcgccgggtt caggtgcgcg tccggagcgg gacttgatgt ccgcgcccgc gcagaagacc
     1981 ttccccgcgc ccgtgagcac aactacccgt gcttcgtcct ggtcgctgat ggcatcgaac
     2041 gtccacgcca tctcggtgag cacctgaccg ctgtgagcat tgaccggcgg attatcgatg
     2101 gtcaccacgg cgatgccgtc cggccgcaat tccgtcttca ccaattcggg tgtcatgaac
     2161 cctcccccaa ggactgctga cggggtccat tgtaacgctc tggatctgag ccgctaccaa
     2221 ggcccggcat cgtggtttaa gcttttccca cggtctggcg gaaccaccgc ggcaatcgga
     2281 ggcagacatg agcaagagcc aacccaacca gctgctgctg accaacgcgc gactctttcc
     2341 cggagtgagc gacgaggtct tcgccgacgg agccgtctgg atcgagggcc gcaccatccg
     2401 ctacgccggc ccgggctgcg atctgcccgc cgtggccgac gacgtgcccc gcgtcgacct
     2461 ccacgggcag ttcgtcatgc ccggcatgac ggagtcccat tctcaccttt cctactccaa
     2521 caacggcccc accgagctgg acaagtctcc ggtggaggac gccatgatcc acgccgtggc
     2581 aaatgcccgt ctgatgctcg gctcgggctt cacgtcggcc atcagcttcg gatccgtgca
     2641 ccgaatcgac gtctttctcc gcgatgccat cgatgcgggc agaataccgg ggccgcgcct
     2701 ggaggcgtcg agccgggacg tgggggcgac gtcgagcaat gccgatcttc acccggacta
     2761 tttcaagccc cagatggaag gcctcggcat gctggccgat gggccctggg ccgtgcgcaa
     2821 ggccgtgcga accatccgca agaaccgcgg caacgtggtc aagctgttcc tggacggcga
     2881 ggggcccggc gggcacgccc cgcccgggga gctgacgtat accgacgagg aagtcgcggc
     2941 agccgtcgac gaggcccacc ggcgcggcat gcgggtcgcc tgccattcga ggtctgccgc
     3001 ggcggtgaag caggccgtgc ggttcgggat cgatttcatc gggcacgcca actacctcga
     3061 cgacgaggcc ctggacatgc tgaaacgaag cagagagcgc ctgttcgttg gtcccgccat
     3121 cgcctgggag ctggcctttc tgcgccacgc cgcggagctc ggctcgcccc cggggtcgca
     3181 gaggtacaag ttctacgagc aggaggtgga agcgaccaag aacgccgtcc gacgccttcg
     3241 cgaagccggc gtgcgggtcc tcatcggcgg ggactacggc ctgagcatca cgcctcacgg
     3301 cacctacgcc aaggacctcc agtacttcgt cgagctcttt ggcctgtcgc cggcggaggc
     3361 gctgctgtgc gcaacccggg acggcggcgc ggcggtcgat ccctccggca tgctcggaac
     3421 gctggaggaa ggcaagctag ccgacctggt gatcgtcgac ggcgaccccc tggaagacat
     3481 caccgtggtg caggatcacg accgcatcac ggcggtcatg aaggacggcg tcgtgcaccg
     3541 ccgcctcgcc gtggacaatc cttaccaggc ctcacccgac gcgctgcagg aggagctggc
     3601 ggcaagagcc gcagaccccg agcgtcagcg gcaggcggcc gttctcctgg agtccagcgg
     3661 ctgaaagcac cagcgacacc cggggctggc atgaccggtc ccgctggatg aacctccaga
     3721 cacttgccac gccccggatc ggctggcgct ggccggcggc cctcattctg tttctgctcg
     3781 gtttcgcggg atttgccaca ggcgtcgcgc cctcagagcg gcccgacgtg gcccaggccg
     3841 atctgttgac caaggcctac tacagcctcg ggctgttcgt ggtgggcggg ctggacatcg
     3901 gcacgccggc gtccggcccg ctgctgggcc gagccatgct ctggatcgcc tacttcggag
     3961 cgcctctgct gacggcctcg gccgtcatcg aggccctggt ccgcgtgatg gcgccgcacc
     4021 gttggcagat gcgacgcctg cacgaccacg tcgtcatcgt gggcgcgggc gagctcacca
     4081 tcagctacct gcgcgtgctg cgggggagtc acccgtcgac cccagtcgtg gtcgtggacg
     4141 aacacgtcga ccccgtgcgc gagcaggaac tggcccaggc gttcgacgtg cgcgtcgtaa
     4201 tcggcaacat cactcacgac ttcgtccaga acgggctgcg cctggaccgc gcggctcgcg
     4261 tcgtcctgct cggcgacagc gacttccagt ccttcgaggc ggcaagcaag atgctgcggc
     4321 gcttccccca tctcgaatca cggctcgttc ttcactgcca gcggctgcgg ttcctgcgat
     4381 caatggaggc aaccaccgtc ggccgcctgt gcatcacgtt caactcctat cacctggcgg
     4441 cggccagcct cgtgaagcaa cagctcatcg gccatttcgc gaagaccgaa caccgcgacg
     4501 tcgtggtgct ggccggcttc ggccgcttcg gccagaccat cctagaggag ctgcagaatc
     4561 tggcccggga cgagctggac accgtggcga tcatcgacgt cgacgcggac cggcgggtga
     4621 tggtcgccga agagcagcgt cggctgggcg gcaactaccg acgggtggta ttgcaggggg
     4681 acatctccca cccggaggtc tggcggtccc tggccgcctc cgtcgatctc tccaaggact
     4741 cgccgacggt gatcctcggc accggccggg ccgaggacaa tctgcgcacg gcgctgtggc
     4801 tcaagcagag ctatccgaac gcgttcgtgt ttgcccgcac gaacgacaag tccgagctcg
     4861 cggtcgaggt gggcagcgag cacgggatca ggaacatcag catccggcag ctcgtggagg
     4921 acaacattcc cgaggagtgg cttgcctgac tgatcgggtc agccgttgag gtggcgggac
     4981 agatctccga cgttttccag gtacacctcg cgtctgacga gcacccagcc ctggatcatc
     5041 agctcgtact gatcgatgta gcgcccgccg acgatgatct cgttggcccc gcccagagac
     5101 tgatagacgg tgaagtagct gcgcacgtcg gcgttgcgaa ccgcgttgtc aaactcgaag
     5161 atgctgttgg tgaccacgtg cttggttcgc ggcgagcagg cgccgcgctg atacggaaca
     5221 acgttctcct cgtcatcgta gaagatgatg acgtcggaaa acgtccgcag cacctcttcc
     5281 ggaccgttca gctcgctgcc gtccggcatg accatgacgc catgggcaaa cagaggcgcc
     5341 acggtttcta gatttcccgt gtcgatgccc tcggcgtagc cgtacatgat gtcctcgatg
     5401 gcgtgccgcc cctgcgaaat cttgaccgtg cgttccatct caccctccgt gccaggcgac
     5461 attatgccca ttcacccgcc atggagacag ccacgggccg cctcggcgct ataatgcagc
     5521 gtcttccggg gggatccaga tcgatgtgca gagcaaggat cgaggcgcca tggccaagcg
     5581 actgatcgtg atggccgggc tgctggcgct ggtcttcggc agcctgttcg gctggaaggc
     5641 gttcgagcgc cacaaaacgg ccgagtatct ggcaaacgcc cggccgccgg cggtggtcgt
     5701 ctcgtcaacg agggtggaag aggaccgctg gcggcagcag gtggaatccg tcggctcgtt
     5761 gagcgccatc cagggcgtgg acgtcagctc cgaggtgccc ggcaccgtcg cggacatcag
     5821 ttttcaatcc gggcagcaga tcagcgcagg cgagctgctc gtccagctcg acgccaccgc
     5881 cgaacgggcc gagctgcgct cgctggaagc ccagctggtg ctggcgcgac tggactacga
     5941 gcgcgcttta ggtctccttg agcgcacgat gttgtcacag gcgcagctcg atcgggccaa
     6001 gagcgtgatg gacagcctgc aggcgcaggt tgaggagcag gccgccttca ttgccaagaa
     6061 ggccatacgg gcacccttcg ccggcgagct cggtatccgg caagtcaacc ttggcgagta
     6121 cgtctccccg ggcacgccca tcgtcactct gcaggcgctt gatcccatct acgcgaactt
     6181 cgcgctcccg gaacggttcc tgcgcgagct cgccgtggac cagccgctgc gcgtggtcgt
     6241 ggccgcctac ccggaggatg agttcctcgg cacggtgacc gccatcagcc ccaaggtcga
     6301 ggagaccacg cgcaacgtcg tggtccaggc taccattgcc aacgccgatc gcaggctccg
     6361 tccaggaatg ttcgcgcgca tcaccgtgca gaccggcggg gaccaccggg tgctgacggt
     6421 tccgcgaacg gcgatcacct tcaatcccta cggcgagtcc gtcttcctca tcaacgagca
     6481 ggacggcgct ctgctggtcg accgccgcca ggtcatcacc ggagagatcc gcaacgagcg
     6541 cgtggaggtc gtttccggcc tgctggccga cgatctcgtg gtggccaccg ggcagctgaa
     6601 gctgagagcc ggacaacgcg tgaagatcga caacagcatc gatctttcca ggccggtgac
     6661 gggcccttga acttcaccga tacgttcgtc cggcgtccgg tgctggccag cgtgatcagc
     6721 ctgctcgtgc tgctggtcgg
//
LOCUS       DBNHQR010000177         6002 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_459933, whole genome shotgun sequence.
ACCESSION   DBNHQR010000177 DBNHQR010000000
VERSION     DBNHQR010000177.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6002)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_459933"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="AC16VR_04015"
     CDS             <1..641
                     /locus_tag="AC16VR_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010354.1"
                     /translation="DDPLVLEASFRGMNSLLRASLNARDIRTTFHLLSQYKAVAEDLV
                     SMGHDEDALAAAEHIQSYGQGAFTMNQPFLLEVAAFDLGELIRLAHKNQSAALDRMLD
                     GFLELDHEIKYESQEESLLGVRRAQMQLGAYLISQGDLARAQRVADDLSSEGASRLDR
                     LQAGLLSDERALFWEFNPRGVNFSYLEPELRPHLNTLMDMVRNEPRRVAPTP"
     gene            complement(662..2053)
                     /locus_tag="AC16VR_04020"
     CDS             complement(662..2053)
                     /locus_tag="AC16VR_04020"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR6010355.1"
                     /translation="MADLLAEQLRLQPVSVTRRMSASLPGLRRLAQEKGLKIHHLGAG
                     YPHPEVTDPRSFIAHREAYFAHLEEREGLNDPRALPEYLRESFSYTDTLGPAGTRAAF
                     ARVYGRDWGVTMDPGRLIPTVGATGGINLACSMFERPGVPLAYITDAPTYAGFNARAT
                     LCQHAAIYSVPMDDEGPLPDVLCDQIRAARADGRFVPFYYTVPDGHNPAGFSFSQARR
                     EAILRVVREEGILILEDAPYVYINFAPAANRPRPFLAMDPRCTIHLFTGSKIGFPGPR
                     VGFLYSEAELAIAGGRTASLTEFALTEASADMLFQNPAALLGFEALLHDDDFSERRSL
                     WPVAETKLAVYRENRSIMLDGLERELGGHRDLFHWTDPKAGFFTVFTFLGPGIRTDDA
                     FVNAMVADHGVVVIPMYDFYPEDARRRDPDAGFDQLRLSFCFSESLGEQRRTDLEEAV
                     AAFCAAAKKLAGV"
     gene            complement(2114..3175)
                     /gene="rsgA"
                     /locus_tag="AC16VR_04025"
     CDS             complement(2114..3175)
                     /gene="rsgA"
                     /locus_tag="AC16VR_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HIR6010356.1"
                     /translation="MNGARPTLPELGWTPFFAEQLSPEEADRLIPARVFGVQRTHITV
                     MAEEGELSMPLGGRWFQLDPQDRPTVGDWVLVSEQRDSVVRLLERRSLLKRLSPGRTG
                     AVQLIAANVDTLFLVTSCNQDFNPRRLERYLALAYESGIEPVVVLTKADLCDDLERYV
                     DGARRIVPGLEVVALDARDRAQLAPLASWLATGRTVALLGSSGVGKSTLVNTLSEAQV
                     QRTAQIREDDGKGRHTTSHRSLHPVPGGGLMLDSPGMRELGMADAELGVRQMFEDVEA
                     LAHQCRFADCAHDSEPGCAVKAALEAGELTEERLESYRKLSREEAVNAETIAERHARS
                     RATDKLHRRVQDASPKRRS"
     gene            complement(3182..3937)
                     /locus_tag="AC16VR_04030"
     CDS             complement(3182..3937)
                     /locus_tag="AC16VR_04030"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR6010357.1"
                     /translation="MTIRLEIDGIEVELRRNSRRRTRIGMAFDPSGFVIMDAPPNVGE
                     NEVREVVAEHHRWLRVRLDKARDAVHEVRALNYVAGELVHYLGQAYRLEVRAGGEVRV
                     ELSAPAHSQLPLFADVAFGTLQVTTPEQSPDGVKTAVCDWYSAQGDRVFAERLAGWSE
                     LPWLNGTTPRWRHTFMRSQWGSCSARGKISLNTHLVKAPVSLVDYVILHELCHLEHHD
                     HGRRFYALMERHMPDWERRRAALDRFLPVLLHD"
     gene            complement(3934..5154)
                     /locus_tag="AC16VR_04035"
     CDS             complement(3934..5154)
                     /locus_tag="AC16VR_04035"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6010358.1"
                     /translation="MTIRSYDEDRDLKAIERIWFECGWLENSRQAAYLKDFLAAGDCL
                     TATIDDTAECAVHVSPGALQYVDEQLELCAVTAVTTSRVARKQGFARELTARQLARGA
                     DDGAEIAALGMFEQGFYDQLGFGTGSYEHEFTFDPATLTVDVPFRPPSRLDRGNWRDI
                     HASLAKRLRGHGGCVLRPSYLIKAELGFSEDGFGLGYFDGSDLTHFFWGEPRGEHGPY
                     RIHFLAYRDAEQLMELLALMRSLGDQVSSIVLLEPPEVQLQDLLDRPFRARRNTRASA
                     HANQHRSIAFWQARVLDVEACVAVRHWPGDEVDFNLVLTDPLSEILAGEAWSGVAGEY
                     VVHVGADSSARSGFEAGLPVLHAGVGAFTRLWLGVVGASALALSDELTGPTELLGRLD
                     EALCLPPARTTWDF"
     gene            complement(5189..>6002)
                     /locus_tag="AC16VR_04040"
     CDS             complement(5189..>6002)
                     /locus_tag="AC16VR_04040"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019474.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6010359.1"
                     /translation="DVEVTEQIIAGPGGDLRLLIYRSPVAEGGPGVLWLHGGGYILGA
                     VDDDRARLVAESLGCTAVSVDYRLAPEHPFPAAVEDAYAALCWLAGNAGALGVDAERL
                     SIAGLSAGAGLTAGLALLNRDRGGPAVAFQLLLSPMLDNLHDTASGRIVNHPTWSRQT
                     SLNAWEMYLDGTPGLDASPYAAPSRATDLSGLPEAYVSVGAEDLFRDECIDYARRLHE
                     HGVATELAVFPGMYHVAENFAPQARVCQRLHGSYLRALADGLGVSRGAADGS"
BASE COUNT         1055 a         2027 c         2014 g          906 t
ORIGIN      
        1 tcgacgaccc gctggtgctg gaggcgagct ttcgcggcat gaacagcctg ctgcgcgcca
       61 gcctgaacgc gcgcgacatc cggacaacct tccacctgct gagccagtac aaggcggtgg
      121 cggaggatct ggtttccatg ggccacgacg aggacgccct cgccgccgcc gagcacattc
      181 agagctacgg gcagggcgct ttcaccatga atcagccgtt ccttctggag gttgccgctt
      241 tcgacctcgg ggagctgatc cgcctggctc acaagaacca gtccgccgcc ctggaccgca
      301 tgctagacgg ctttctggag ctggatcacg agatcaagta cgaatcccag gaggagagcc
      361 tgctcggcgt gcgccgggca cagatgcagc tgggcgccta cctgatcagt cagggcgacc
      421 tggcccgggc ccaacgggtg gccgacgacc tgagctcgga aggcgcctcc cggctggatc
      481 ggctgcaggc cggactgctg agcgacgaac gggccctgtt ctgggagttc aaccctcggg
      541 gcgtaaattt cagctacctg gagcccgagc tgcgtcccca cctgaacacg ctcatggaca
      601 tggtccgcaa cgagccgcga agggtggccc cgacaccctg aggcgacgga gcctcggcgt
      661 ctcagacccc ggcgagcttc ttcgccgccg cgcagaaggc ggctaccgct tcctcgagat
      721 ccgtgcgccg ctgctcaccc aggctttcac tgaagcagaa cgacaaccgc agctggtcga
      781 agccggcatc ggggtcgcgg cgccgggcat cctccgggta gaagtcgtac atcgggatca
      841 ccaccacccc atggtcggcc accatcgcgt tgacgaaggc atcgtccgtg cggattcccg
      901 ggccgagaaa cgtgaaaaca gtgaagaagc ccgccttggg gtcggtccag tgaaacagat
      961 cccgatgccc gccgagctcc cgttccaggc cgtcgagcat gatcgaccgg ttctcgcgat
     1021 acacggcgag cttcgtctcc gccaccggcc acaacgaccg ccgctcgctg aagtcgtcgt
     1081 cgtgcagcag cgcttcgaag cccagcaggg cggccgggtt ctgaaacagc atgtcggcgc
     1141 tggcttcagt cagcgcaaat tcggtcagcg aagccgtccg cccgccggca atggcaagct
     1201 cggcttccga gtagagaaag cctacgcgcg gcccgggaaa gccgatcttg gatccggtga
     1261 agaggtggat cgtgcagcgc gggtccatgg cgaggaaggg ccgggggcgg ttcgcggccg
     1321 gcgcaaagtt gatgtagacg taaggagcgt cctcgagaat gaggatgccc tcctccctga
     1381 cgacccggag aatggcttcc cgccgtgcct ggctgaacga gaaccctgcc gggttgtgcc
     1441 cgtccggcac cgtgtagtag aacggcacga agcggccgtc ggcccgcgcg gcgcggatct
     1501 gatcacagag aacgtccggc agcgggcctt cgtcatccat gggcacgctg tagatggcgg
     1561 cgtgctgaca cagcgttgca cgggcgttga atccggcgta ggtcggggcg tcggtgatgt
     1621 aagccagcgg cacgcccggg cgctcgaaca tggagcaggc gagattgatg ccgccggtcg
     1681 cccccaccgt cggtatgagg cgcccgggat ccatcgtgac tccccagtcc cggccgtaaa
     1741 cgcgggcaaa cgccgcccgc gtgccggccg ggcccagcgt atccgtgtag gagaacgact
     1801 cccgcaggta ctcgggcagg gctcgagggt cgttgaggcc ttcccgctct tcgaggtggg
     1861 cgaagtaggc ttcccgatgg gcaatgaacg accggggatc ggtcacttca ggatggggat
     1921 accccgctcc caggtggtgg atcttcaacc ccttctcctg agcgagcctg cgcaggccgg
     1981 gcaaagaggc agacatgcga cgggtgacgg aaacgggctg caggcggagc tgctcggcga
     2041 gaaggtcggc catgacgcgc ctcgggcgga agaggcgcgt actttaaggc atctccggct
     2101 cccgaggtag gcgtcagctg cggcgcttgg gagatgcgtc ctggacccgc cggtgcagct
     2161 tgtccgtcgc tcgggagcgg gcgtgacgtt cggcaatggt ttccgcgttc acggcttctt
     2221 cccggctgag cttgcggtaa ctctccagcc gctcctcggt aagctccccg gcctcgaggg
     2281 ccgccttgac cgcgcagccg ggttccgagt cgtgcgcgca gtcggcaaag cggcactggt
     2341 gcgcgagcgc ttccacgtcc tcgaacatct gacgcacgcc gagttccgca tccgccatgc
     2401 cgagctcccg catacccggg ctgtccagca tcagcccgcc gccgggcacc gggtgcagcg
     2461 agcggtgcga ggtggtgtgc ctgcccttcc cgtcgtcttc ccggatctgc gccgtgcgct
     2521 gcacctgagc ctcggagagg gtgttgacca gcgtggattt acccacaccc gacgagccga
     2581 gcagcgccac cgtgcggccg gtcgccaacc aggacgcaag gggagcgagc tgtgcccggt
     2641 cgcgagcgtc cagagccacc acctcgaggc ccgggacgat ccgtcgggcc ccatccacgt
     2701 agcgctccag atcgtcgcat agatccgcct tggtgagcac caccacgggc tcgatgcccg
     2761 actcgtatgc cagcgccagg taacgctcga ggcggcgcgg attgaaatcc tgattgcacg
     2821 aggtgaccag gaacagcgta tcgacgttgg ccgcgatgag ctgcaccgca cccgtgcggc
     2881 ccgggctcaa tcgcttgagg aggcttctgc gttcgagcag cctcaccacg ctgtcgcgct
     2941 gctcagaaac cagcacccag tcgcccaccg tgggccggtc ctggggatcg agctgaaacc
     3001 agcgcccccc caggggcatg gaaagctcac cctcctcggc catcaccgtg atgtgagtcc
     3061 gctgcacgcc gaaaacccgg gcggggatga gccggtctgc ctcctcggga gacagctgct
     3121 cggcgaagaa cggtgtccag ccgagctcgg ggagagtggg cctggcgccg ttcacgagag
     3181 atcagtcatg gagaagcacc ggcaggaagc ggtccagggc ggcgcggcgg cgctcccagt
     3241 cgggcatgtg ccgctccatc aacgcgtaaa agcgccgtcc gtgatcgtga tgctcgagat
     3301 ggcacagctc gtgaagaatc acgtagtcga cgagagacac gggggccttg accagatggg
     3361 tgttgagcga gatcttgccc cgcgcgctgc agctgcccca ctggctgcgc atgaaggtgt
     3421 gccgccagcg cggcgtcgtt ccgttgagcc aaggaagttc cgaccacccc gccagccgtt
     3481 cggcgaagac ccgatcgccc tgtgccgagt accagtcgca cacggcggtt ttgaccccgt
     3541 ccggcgactg ctcgggcgtc gtcacctgca gggtaccgaa cgccacgtcg gcaaacagcg
     3601 gcagctggga atgggcaggc gcgctgagct ccacccgcac ctcccccccg gcgcgaacct
     3661 cgagccggta cgcctggccg agatagtgga cgagctcgcc ggcaacatag ttgagcgcgc
     3721 ggacttcgtg aaccgcgtcg cgcgccttgt cgagcctgac gcgcagccag cgatgatgct
     3781 cggcgaccac ctcccggact tcgttctcgc cgacgttggg gggcgcgtcc atgatcacga
     3841 atccgctggg gtcgaaagcc atgccgatac gggtgcgacg ccgggagttg cggcgcagct
     3901 ccacctcgat gccgtcgatt tccagccgaa tcgtcaaaag tcccaggtcg ttctggccgg
     3961 cggcaggcag agagcctcgt ccagccggcc gagcagctcg gtcgggcccg tcagctcgtc
     4021 gctcagagca agagccgaag cgccgaccac cccgagccac aatctcgtaa acgcaccgac
     4081 gcccgcatgc agcaccggca gcccggcctc gaagcccgat cgggcagagg agtcggcgcc
     4141 cacgtgcacc acgtactcac ccgccacgcc cgaccaggct tctcctgcca ggatctcgct
     4201 caaggggtcg gtgagcacga ggttgaagtc gacctcgtcg cccggccagt gccgaaccgc
     4261 cacgcaggcc tcaacgtcca gcacccgcgc ctgccagaac gcaatggacc ggtgctggtt
     4321 ggcatgggcg gaagccctgg tgttgcggcg cgcgcgaaat ggccgatcca ggagatcctg
     4381 cagctgcact tcaggtggct ccagcaggac gatggaggac acctgatcac cgagggagcg
     4441 catcagcgcc agaagctcca tgagctgctc ggcgtcgcgg taggccagga agtgtatgcg
     4501 atagggtccg tgctcgccgc ggggttcgcc ccagaagaag tgggtgagat cggatccgtc
     4561 gaagtacccg aggccgaacc cgtcttccga gaagccgagc tccgccttga tcagatacga
     4621 agggcgcagc acgcagccgc cgtggccgcg aagcctcttc gccagggagg catgaatgtc
     4681 gcgccagttg cctcgatcga gccggctcgg gggtcgaaac ggcacgtcta ccgtcagggt
     4741 cgccgggtcg aacgtaaact cgtgctcgta gctgcccgtg ccgaacccga gctggtcgta
     4801 aaagccctgc tcgaacatgc cgagcgccgc gatctcggca ccatcgtccg ctccgcgggc
     4861 cagctggcgc gccgtgagct cgcgggcaaa cccctgcttg cgcgcgaccc ggctggtagt
     4921 cacggcggtc accgcgcaca gctcgagctg ctcgtcgacg tactgcaagg cgccggggga
     4981 aacatgcacc gcgcactccg ccgtatcgtc gatggtggcg gtgaggcagt cgcccgcggc
     5041 gaggaagtcc ttgagataag cagcctgacg cgaattttcc agccacccgc actcgaacca
     5101 gatgcgctcg atggctttga ggtcccggtc ttcgtcgtag ctgcggatcg tcataggcac
     5161 gcagggcgcg gaaaacccgg attatgcgtc aggagccgtc ggccgcgcca cgcgacaccc
     5221 ccagcccgtc cgccagggcg cgcagatagc ttccgtgcag ccgctgacag acccgcgcct
     5281 ggggcgcgaa gttttcagcc acatggtaca tgccgggaaa cacggccagc tccgtggcca
     5341 ccccgtgctc gtggagccgg cgggcatagt cgatgcactc gtcccggaac agatcttccg
     5401 cgccaacgga tacgtacgcc tcagggagac cggagaggtc ggttgcgcgg ctgggagccg
     5461 catagggtga ggcgtccagc ccgggcgtgc cgtccaggta catctcccag gcgttcagcg
     5521 acgtctgcct ggaccaggtc gggtggttga cgatccggcc cgaggcggtg tcgtggaggt
     5581 tgtcgagcat gggggaaagc agaagctgaa aggcgaccgc cggccctccc cggtcgcgat
     5641 tgagcagggc aagccccgcc gtcagccccg cccctgcgct gagcccggca atggaaaggc
     5701 gctcggcgtc cacaccgagc gcgccggcgt tcccggccag ccagcacaac gcagcgtagg
     5761 catcttcaac cgccgccggg aaaggatgct ccggcgccag acggtaatct acggataccg
     5821 ccgtgcaccc gagcgattcg gcaacgaggc gtgcccggtc gtcgtccacc gctcccagga
     5881 tgtagccgcc gccgtggagc cacagaaccc ccgggccgcc ctcggcaacg ggagaccggt
     5941 agatcaggag ccgcaggtcg ccgcctggcc cggcgatgat ctgctcggtg acttccacgt
     6001 cc
//
LOCUS       DBNHQR010000178         6778 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_587463, whole genome shotgun sequence.
ACCESSION   DBNHQR010000178 DBNHQR010000000
VERSION     DBNHQR010000178.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6778)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6778)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6778
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_587463"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..2208
                     /locus_tag="AC16VR_04045"
     CDS             118..2208
                     /locus_tag="AC16VR_04045"
                     /inference="COORDINATES: protein motif:HMM:NF015722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HIR6010360.1"
                     /translation="MSTRNGQDNNASQVQADDLEIIEAVSYTPREQAPKRKLPLTPFQ
                     IGAVLVAIPVMAIMWFLFTAKSVRLEFDPPAASESVSEGPAIALGDIYLMLDGEYRVS
                     ARAEGYEPLDELLVVGDARSQTHRFEFTKLPGRVTFLSNPDGAEVIVAGALLGRTPLD
                     GVKVPAGPATISMKLDRYQRLDMEAEIEGLDRAQTVEGSLLPDWAEVSVSSIPAGAEI
                     LLNDQPTGRLTPAVIEVPTGEHEIRLKAPGHRGHRQRILVAALDEVTLPEVTLLRADG
                     LLQVTSRPAGAGVTLNGQFQGETPVEIAVKSGISYRVKIFKAGFAPGERSVRVASGGE
                     RTVDLTLTRLTGQLIVTTQPAEAELFVDGRNLGNANQTLTLPTVTHRVEIALDGYAGY
                     STEITPRNGVTQEIKVRLLTLEEARLAAMKPVIQTSQGQELVLLNPGPFTMGASRREP
                     GRRANETLREVGMERIFYLSRTEVTNAQFLDFAPGHDSGEFQDHELNKDDQPVVGVSW
                     QEAALYCNWLSAREGLPPFYATDLGKVVATNPAATGYRLPTEAEWEWAARQNGPDSTG
                     YRFPWGTALPPPDRHGNYADRSAAHLVGRVVFGYNDNHIVSAETGTFAPNAIGIYDLS
                     GNVAEWVNDFYEIPGAEPTKDPLGPDTGEYRVIRGSSWMNGTITDLRLSFRDYGVDGR
                     EDVGFRIARFAEPS"
     gene            2205..2411
                     /locus_tag="AC16VR_04050"
     CDS             2205..2411
                     /locus_tag="AC16VR_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010361.1"
                     /translation="MKGPLLFAAAALLASTMGAGFAVAAEEAADTRDRAGEEEEADGE
                     PSPEIFVPSEEISEDFAVSFPVDI"
     gene            2433..3233
                     /locus_tag="AC16VR_04055"
     CDS             2433..3233
                     /locus_tag="AC16VR_04055"
                     /inference="COORDINATES: protein motif:HMM:NF013760.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR6010362.1"
                     /translation="MKRQLPFELIYQSSALVLAVILVHLVYVTVIRPNADAIIAAQAE
                     RAAAGEAYAQERSLYVVLKDYEQESCFVLMLWAMAIMGYKTRSALRERSLLQEDIVKI
                     SEGMSVLPEDTREYARAVQALPDENRNYLLPRALMAALQRFASTRNVQDVSEAVRSVC
                     ESESDRLDSELSMVRYIAWAIPSIGFIGTVRGISEALGQAYEAVEGNIAGVTTSLGVA
                     FNSTFTALVISIVIMFLMHQLQLIQERLVLDAQTYCDERLLRHLQVRD"
     gene            3233..4483
                     /locus_tag="AC16VR_04060"
     CDS             3233..4483
                     /locus_tag="AC16VR_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010363.1"
                     /translation="MDYVLELNDSDLSLHSGETLAYHAPGVALVRENEILFGDPALRL
                     SRAHPRQINQHYLARMNADPLPYPVSNAANHADLVYLHLKQLQDLMRRDPGDLVLAVP
                     AILSADQLGVLLGITQECGMAVSGFVDSAVAAASVSELPASVCHLDAHLHRTCLTLLD
                     VGADVVRTGADEVADCGVANLLEGWVNVIADRFVRDTRFDPLHAAETEQQLYNQVYDW
                     AMGQSSSDSEIGIEIGYRDQVRRVEVSRSLLEEKARQRFAHLSERLPEDVHLALSART
                     ARLPGLTAYLGAQGWEYSVLDGSALAAGCSANDSSIRAEDGRLRLVSRLPLRHGRGPQ
                     SIVAPGPDESGRPTHVLCDAWAVPIGADDLPLQLKESEAGVYLDADETVRLNGEAITT
                     SRRLRAGDELTAGGKSYLLIRVVG"
     gene            4485..5573
                     /locus_tag="AC16VR_04065"
     CDS             4485..5573
                     /locus_tag="AC16VR_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR6010364.1"
                     /translation="MARNRRFSVFSLSFLDVMSCGFGAVVLIFLIINHAAEEDAKVVN
                     EELLSEIRLLDYQVQNGERDLFELKEQLDAILQRLSDSSRKLVSTETSLKETSKEFAD
                     LAAESLAQEASLEELKSDVDTREEELARLKSINEANEGGRIRSFTGEGDRQYLTGLKV
                     GGKHILIAVDTSTSMLDESIVNVIRRRNMPDDRKRMSPKWQRAVRTVEWLTAQLPLDA
                     SFQIYGFNVEARSLLEEHHGQWIALAEGRELDDALEALREVIPDRGTSVANLISAVNE
                     LNPPPDNVYLIIDSLPTQGTREPRRATISGGDRLKLFADAIQTLPKQVPINVIMFPME
                     GDPMAPAAYWNLARISGGAFLSPSRDWP"
     gene            5570..6562
                     /locus_tag="AC16VR_04070"
     CDS             5570..6562
                     /locus_tag="AC16VR_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR6010365.1"
                     /translation="MKTRNRRAIEEFSVSFLDVICCGFGAIILLLMITKTVEPILLEK
                     SQVNLEGQVADREQALFEIQGQIKELRRQVDDTSGQLDDNLLELARLEQQLTIILGKF
                     ETTAARQDDNLTETQKLAAAKQSLTDEMQRLLGVDFRRSTDLVGGIAVDSEYIIFVID
                     TSGSMYNAAWPQVLKKVNETLSIYPRVKGIQVMNDMGDYMFARFAGEWIPDSPSRRKA
                     ILDRLQTWNPFSNSSPVEGIQAAINTYYDPDKRISIYVFGDDFTGESIESVVDAVDRV
                     NVADDSGRRRVRIHAVAFPVYLSRPSGRVYRFAALMRELAYRNDGTFVGLSEFQ"
BASE COUNT         1274 a         2192 c         2138 g         1174 t
ORIGIN      
        1 gctcctacac ggtcattggc agccgtgacg gctgccgcga tgtcagggcc gacatcgtcg
       61 tcaaaccgaa catgcagccc gttgacatcc gctgcaccga gcggctctag gcttgtcgtg
      121 agcactcgaa acggccagga caacaacgcc tcccaggttc aggccgacga tctggagatc
      181 atcgaggcgg taagctacac gcctcgggag caggcgccga aacgaaagct gccgctgacg
      241 ccgtttcaga tcggcgccgt gctcgtagcg attcccgtga tggcgatcat gtggtttctc
      301 ttcaccgcga aatccgtgcg gctggagttc gaccccccgg ccgcctcaga gagcgtgagc
      361 gaaggtccgg ccattgccct gggcgacatt tatctgatgc tcgacggcga gtatcgggta
      421 agtgctcgcg ccgagggcta cgagccactc gacgagctcc tcgtcgtcgg cgacgcgcgc
      481 agccagacac atcgcttcga gttcacgaag cttcccggtc gggtcacctt tctgagcaat
      541 cccgacggcg cggaggtcat cgtggccggg gcgctgctcg gacgaacgcc actggacggt
      601 gtcaaggtcc ctgccggccc ggcgacgata tccatgaagc tggaccgcta ccagcgtctg
      661 gatatggagg ccgagatcga aggtctggat cgagctcaga ccgtggaggg ttccctgctt
      721 cccgactggg ccgaggtgag cgtttccagc atcccggccg gagcggagat cctcctgaac
      781 gaccaaccca cgggtcgtct taccccagcc gtcatcgagg tgcccaccgg cgagcacgag
      841 ataaggctca aggcgcccgg ccaccgcggg catcggcaac gcatcctggt cgccgcgctg
      901 gatgaggtga cgctgccgga agtcaccctg ctccgcgccg acgggctgct gcaggtcacg
      961 agccgaccgg ctggagcggg cgtcacgctg aacgggcagt ttcagggcga gacgcctgtg
     1021 gagatcgcgg tgaaatccgg tatcagctac cgggtgaaga tcttcaaggc aggtttcgcc
     1081 cccggcgagc gcagcgttcg ggtggcctcc ggcggtgagc gcacggtaga cctgacgctc
     1141 acgcgcctta caggtcagct catcgtcacg acccagcccg ccgaagccga gctcttcgtc
     1201 gacggccgta atctgggcaa cgccaaccag acgctgaccc tgcccaccgt gacccatcgg
     1261 gtcgagatcg cgctggatgg atacgcgggt tactccacgg agataacgcc gcgcaacggg
     1321 gtcacccagg aaatcaaggt ccgccttctg accctggaag aggctcgtct ggcagccatg
     1381 aagccggtca tccagaccag ccagggccag gaactcgtgc tgctgaatcc gggccccttc
     1441 accatgggcg cttcccggcg cgagccaggc cggcgagcca acgagacgct ccgagaggtg
     1501 ggcatggagc ggattttcta tctcagccgg accgaggtga ccaacgcgca gtttctcgat
     1561 ttcgcgcccg ggcatgactc gggggaattt caggatcacg agctcaacaa ggatgatcag
     1621 ccggtggttg gcgtctcctg gcaggaggcc gccctctact gcaactggtt gtcggccagg
     1681 gaagggctac ccccgttcta tgccacggac cttggcaaag tggtcgcaac gaaccccgcg
     1741 gctaccggct accggttgcc caccgaagcg gagtgggagt gggcggcccg ccagaacggc
     1801 ccggacagca ccggatatcg tttcccgtgg ggaaccgcgc tgccgccccc cgatcgtcac
     1861 ggcaactacg ccgaccggtc ggccgcacac ctggtggggc gcgtggtgtt cggctacaac
     1921 gacaaccaca tcgtctccgc cgagaccggg acgttcgcgc ccaacgccat cggcatctat
     1981 gatctgtcgg gcaacgttgc ggagtgggtg aacgacttct acgagattcc gggggctgaa
     2041 ccgacgaagg atcccctggg accggacacc ggggagtatc gcgtgatcag aggatccagc
     2101 tggatgaacg gaaccatcac tgacctgcgc ctgtcgtttc gcgactacgg tgttgatggt
     2161 cgcgaagacg tcggttttcg catcgcgcgg ttcgcggagc cgtcgtgaaa ggccctctcc
     2221 tcttcgcggc ggcagcgctg cttgcgtcga ccatgggcgc cgggttcgcc gtggctgccg
     2281 aggaagctgc cgacacccgc gatcgcgccg gggaagaaga ggaggcggac ggcgagccca
     2341 gccccgagat cttcgtgccc agcgaggaga tttcggagga cttcgcggtc tcattccccg
     2401 tagacatcta atcgcgtctg gaaccacagc aaatgaagcg tcagctaccc tttgaactca
     2461 tttaccagtc gtcagcgctg gtccttgcgg tgatcctcgt tcatctggtc tacgtcacgg
     2521 tcatcagacc gaatgccgac gccatcatcg ccgcccaggc cgaacgtgct gcagccggcg
     2581 aggcatacgc ccaggagcgc tccctttatg tggtgctcaa ggactacgag caggagtcct
     2641 gtttcgtgct gatgctctgg gccatggcca tcatggggta taagacccgc agcgcgttgc
     2701 gcgagcgaag cctgctgcag gaagacatcg tcaagatcag tgaaggcatg agcgttctcc
     2761 cggaggacac tcgggagtac gcgcgggccg tccaggctct gccggacgag aatcgaaact
     2821 acctgctgcc gagggccctg atggctgccc tgcagcggtt tgcctccacc cgtaacgtgc
     2881 aggacgtctc cgaagccgtg cggtcggttt gcgagtcaga gtccgatcgg ctcgacagcg
     2941 agctgtccat ggtccgctac atcgcgtggg caattccctc catcgggttc atcggcacgg
     3001 taaggggtat cagcgaggcc ctcgggcagg cctacgaggc tgtcgagggc aacattgcgg
     3061 gcgtcaccac gagcctgggc gtcgcgttca actcaacctt taccgctctg gtgatcagca
     3121 tcgtgatcat gttcctcatg catcagctcc agctcatcca ggagcgactg gttctggacg
     3181 cccagactta ctgcgacgag aggctgctgc gtcatcttca ggtgcgcgac tgatggacta
     3241 cgtcctcgag ctcaacgact cggacctgtc gctgcactcg ggcgagacgc tggcctatca
     3301 cgcgccggga gtcgctctgg tccgggaaaa cgagatcctc ttcggcgacc ccgcgcttcg
     3361 gctgtcccgc gcacaccccc gccagatcaa ccagcactac ctcgcccgca tgaacgcgga
     3421 cccgctcccc taccccgttt ccaacgccgc taatcacgcg gatctggtct acctgcacct
     3481 caagcagctt caggacctga tgcggcgcga cccgggagat ctcgtgctgg cggtacccgc
     3541 gatcctgagc gcagatcagc tcggcgtgct gctgggcata acccaggagt gcggcatggc
     3601 ggtgagcggc ttcgttgact ccgcagtagc agcggcgtcg gtcagcgagc tgccggccag
     3661 cgtctgccat ctcgacgcgc acctgcacag gacgtgtctt accctgctgg atgtcggcgc
     3721 cgacgtcgtt cgaacggggg cggatgaggt tgccgactgc ggcgtggcca acctgctgga
     3781 aggctgggtc aacgtgattg ccgaccgctt cgttcgggac acgaggttcg accctctgca
     3841 cgccgccgag acggagcagc aactctacaa tcaggtctac gactgggcca tgggccagtc
     3901 gagcagcgac agcgagatcg gcatcgagat cggctaccgg gatcaggtca ggcgggtaga
     3961 ggtctcccgc agtctgctcg aggagaaagc ccggcagcga ttcgcccacc tcagcgaacg
     4021 cctccccgag gacgtgcacc tggcgttgag cgcgcgtacg gcaaggctcc ccgggctgac
     4081 cgcctatctg ggcgcccagg gttgggaata ctcggtactc gacggctccg ccctggcggc
     4141 gggctgcagt gcgaacgact ccagcatccg ggccgaggac ggccggctca ggctggtttc
     4201 ccggctgcca ttacggcacg gccggggtcc gcagagcatc gttgccccgg ggcccgacga
     4261 gtccggccgc cctacccacg ttctctgcga tgcctgggcc gtgcccatcg gtgccgacga
     4321 tctgccgctg cagctcaagg agagcgaggc cggcgtgtat ctcgatgccg acgagacggt
     4381 ccgtctcaac ggcgaggcga tcaccaccag cagacgcctg cgcgcggggg atgagctcac
     4441 cgccggcggc aaaagctatc tcctgatccg cgtggtgggg tgaggtggcc cgcaaccggc
     4501 gcttcagcgt cttctctctg tcgtttttag acgtgatgtc gtgcgggttc ggggccgtgg
     4561 tactgatctt cctgatcatc aatcacgcag ccgaggagga cgccaaggtc gtcaacgagg
     4621 aactcctttc agagattcgc ctgctcgact atcaggtgca gaacggtgag cgggaccttt
     4681 tcgagctcaa agagcagctc gacgccattc tgcagcggct gtccgactcg agccgcaagc
     4741 tcgtcagcac ggaaaccagc ctgaaggaaa ccagcaaaga gttcgccgac ctcgccgcgg
     4801 agtcccttgc ccaggaagct agcctggaag agctgaagag cgacgtggac acgcgcgagg
     4861 aggagctggc caggctcaaa tccatcaacg aggcaaacga gggaggcagg atacgcagct
     4921 tcaccggcga gggggatcgg caatacctga ctggcctgaa ggtcggcggc aagcacatcc
     4981 tcattgccgt ggacacgtcg accagcatgc tcgatgagag catcgtcaac gtgattcggc
     5041 gccgcaacat gcccgacgat cgcaaacgga tgtcgcccaa gtggcaacgg gccgttcgca
     5101 ccgtcgagtg gctgaccgcc cagctgcccc ttgacgccag tttccagatc tacggattca
     5161 acgtggaggc gcgatcgctg ctggaagagc accatggaca gtggattgct cttgccgagg
     5221 gccgcgagct ggacgacgcg ctggaagctc tgcgcgaggt gatacccgac cggggaacca
     5281 gcgtcgccaa cctgatcagc gccgtcaacg agctgaatcc gccgccggac aacgtttatc
     5341 tgatcatcga ctccctgccg acccagggca cccgcgagcc gcgccgggcc accatctccg
     5401 gcggcgatcg gctgaagctg ttcgcagacg ccattcagac acttcccaag caggtgccca
     5461 tcaacgtgat catgtttccc atggagggag atcccatggc cccagccgcg tactggaacc
     5521 tggcgcgcat atccggcggg gccttcctct cgccatcacg cgactggcca tgaagacccg
     5581 caaccgaaga gccatcgaag agttcagcgt ctcgtttctc gacgtgatct gctgcggttt
     5641 cggcgccatc atcctgctcc tgatgatcac caagacggtt gagcccatcc tgctcgagaa
     5701 atcccaggtc aatctcgaag gccaggtcgc cgaccgcgag caggctcttt tcgagataca
     5761 gggacagatc aaggagctgc gccgccaggt cgacgacacc agcgggcagc tggatgacaa
     5821 cctgctggag ctcgcacgcc tcgagcagca gctcaccatc attttaggca agttcgaaac
     5881 caccgcggcg cgccaggacg acaacctcac ggagacccag aaactggccg ccgccaagca
     5941 gtcgctcacc gacgagatgc agcgtctgct cggtgtcgac ttccgacgca gcaccgacct
     6001 ggtcggcggc atcgccgtcg acagcgagta catcatcttc gtaatcgaca cgtcgggcag
     6061 catgtacaac gcggcctggc cccaggttct gaagaaagtg aacgagaccc tttcgattta
     6121 tccccgggtg aagggcatcc aggtgatgaa cgacatgggg gattacatgt tcgctcgctt
     6181 cgccggcgag tggattcccg actcaccgag ccggcgcaaa gccatcctcg accgcctgca
     6241 gacgtggaac ccgttcagca attcctcgcc cgtggagggg atccaggctg ccatcaacac
     6301 ctattacgat cccgacaaga gaataagcat ctacgtcttc ggcgacgatt tcaccggcga
     6361 atccatcgag tccgtcgtcg atgccgtgga ccgggtcaac gtggccgacg actcggggcg
     6421 ccggcgcgtg cgcattcacg ccgtcgcttt ccccgtatat ctctcgcggc cgtccgggcg
     6481 ggtatatcgc ttcgccgctc tgatgcggga gcttgcctat cgcaatgacg gaacttttgt
     6541 agggctgtcg gaatttcagt agtcttaaag ggtcgaaaga tcagggacaa tgggagctgc
     6601 cgcgctgtga gcaaacgacc tccggcatgg tcgaattcgc ccccgctcgg taggggagcg
     6661 cggcttcgtg tcgccctgct gacagccgtc tgcatggctt ttggtacggc ctgcggaggt
     6721 gccagcgtga cggttcccac gaacttcccc gtacccgtgc tggacaagct cccgctga
//
LOCUS       DBNHQR010000179         3315 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1220791, whole genome shotgun sequence.
ACCESSION   DBNHQR010000179 DBNHQR010000000
VERSION     DBNHQR010000179.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1220791"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="AC16VR_04075"
     CDS             <1..835
                     /locus_tag="AC16VR_04075"
                     /inference="COORDINATES: protein motif:HMM:NF024491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HIR6010366.1"
                     /translation="NSRWAYPVSALMEFSLLPVDLETIRQRIRDRLEEWPDLESLGRE
                     IAFEDTLADLRDAAALDWSTTVVDDPEVGWFERPDDIALDLTELALRAEREVLVVTPY
                     LIPTPNAFKIGKELVDKGVRVRIVTNSLGTNDVVAAQAAYGRYRKRILDTGVELYELR
                     ADAALPERGLSEEVCLHSKYIIIDDEIVFVGSLNLDPRSLYLNTEVGVVLESRPLAAT
                     LRESFDLLAHPDSSWRVTTHENGFRWESSAGTLDRQPAKNGWQRFRYWFLSLIPVSSQ
                     L"
     gene            971..2089
                     /locus_tag="AC16VR_04080"
     CDS             971..2089
                     /locus_tag="AC16VR_04080"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6010367.1"
                     /translation="MSYPIISADSHITEHPDTYSAHIDPAWRDRAPKMIDGGERGDLF
                     VIDGMDTPIAMGLVAAAGKPAEELTMAGVRFEELHRSGWDPAARMADQDRDGVAAEII
                     YPTVGMLLCNHRDFDYKKACFDAYNRWIAEYCSTNPARLLGCGQTAVRSPAETVSDLE
                     KIRELGLRGVMMPGDPLEEDYDSQIYNPVWEAAIDLGLPLSFHILTTRQQAPVRGPRI
                     NSFLSIIRGNQDIMGTLIFGGVFDRYPELKVVCVEADAGWVPHYMYRMDHAYKRHRNW
                     LAKGVSLARLPSEYFSEHVYTTFQDDWVAFRLVNDMNWRRLLWANDFPHSDSTWPWSQ
                     QMLAEQTANLTEEQKRGILCDNVAELYRIDVDALAQAA"
     gene            2089..2412
                     /locus_tag="AC16VR_04085"
     CDS             2089..2412
                     /locus_tag="AC16VR_04085"
                     /inference="COORDINATES: protein motif:HMM:NF049702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7508 domain-containing protein"
                     /protein_id="HIR6010368.1"
                     /translation="MSIRLTKPWSPASAAGKLPGQMGVYQLGDEAGNVITIGYAGGRS
                     TFGLRGEVETALQRHPDAVSYRVEVNSAYLSRYRELLMVHLADFGTLPPGNEALPGLG
                     RLSPA"
     gene            2422..>3315
                     /locus_tag="AC16VR_04090"
     CDS             2422..>3315
                     /locus_tag="AC16VR_04090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6010369.1"
                     /translation="MDLDLSEEQNLLVGTVRRFVREEVLPLEQSLDPDADELEPDDHE
                     RLTQLTREMGLYGLDVPPEFGGPDVDLVTRTLLAMEMSQHRAGLYAPCYGVFGGAGLA
                     QLFEATEDQKDRYLFPLLRGEKKGFFGLTEPSGGSDPARAIQTRAVRDGDEWVVNGSK
                     IFISGADRADFGLVFARTDADKGRNGVTCFIVDTDTPGFHVRRIVHTLRTARYATELS
                     FEDMRVAHGNVLGEVNGGFAIANDRLSRQRIPYAAGCIGVAIKAHEMALEYVPQRETF
                     GAPLSTRQAIQWMLVDNEIDIR"
BASE COUNT          571 a         1061 c         1105 g          578 t
ORIGIN      
        1 gaacagccgc tgggcttatc ccgtgagcgc gctgatggag ttctccctgc tgccggtgga
       61 tctggagacg atacgtcagc ggatccgtga ccgcctcgag gagtggcccg atctggaatc
      121 gctcggccgc gagatcgcgt tcgaggacac ccttgccgat ctgcgcgacg ctgcggcgct
      181 ggactggtcg accacggtgg tcgacgaccc cgaggtcggc tggttcgaac gcccggacga
      241 cattgccctg gatcttacgg agctcgcgct gcgtgccgag cgcgaggtgc tggtggttac
      301 tccttacctg atccccacgc ccaacgcctt caagatcgga aaggagctcg tcgacaaggg
      361 ggtgagggtg aggatcgtca ccaactccct gggaaccaac gacgtggtcg cggcccaggc
      421 ggcctacggg cgctaccgca aacgcatcct ggatacgggc gtcgagcttt acgagctgcg
      481 ggcagacgcg gcgttgccgg agcgtgggct ctccgaggag gtctgcctgc actccaagta
      541 catcatcatc gatgacgaga tcgtcttcgt gggctcgctg aacttggatc ccaggtccct
      601 ctacctgaac acggaggtcg gggtggtgtt ggagtcgcga cctctggcgg cgacgctgcg
      661 agagtccttc gatctgctcg cccatcccga cagctcatgg cgggtcacga cccacgagaa
      721 cggattccgc tgggagtcgt cggccggaac gctcgatcgc cagcctgcaa agaacggctg
      781 gcagcgtttc cgttactggt tcttgagcct catcccggtc tccagccagc tctgaggcct
      841 ggggttcggg gctttcgccg cgcgcggcga aggggcgcgg gcggcccgct gatccgtccg
      901 cggcgccttg acaggcagcg ccgtcggccg ggatagtcgg tggcgtgatc gtcatcaaag
      961 gggagagacg atgagctatc cgatcatctc ggccgattcc cacatcaccg agcatccgga
     1021 cacctactcg gcgcacatcg atccggcctg gcgggatcgc gctccgaaaa tgatcgacgg
     1081 cggtgagcgg ggcgacctct tcgtcatcga cgggatggac accccgattg ccatggggct
     1141 ggtggctgcg gctggaaagc ctgcggagga actcaccatg gcgggtgtgc gcttcgagga
     1201 gctgcaccgc agcggctggg atccggcagc ccgcatggcg gatcaggacc gggacggggt
     1261 agccgcggag atcatctacc cgaccgttgg catgcttctg tgcaaccatc gggatttcga
     1321 ctacaagaag gcctgcttcg acgcttacaa ccgctggata gccgaatact gctcgacgaa
     1381 cccggcccgc ctcctgggct gcggccagac ggccgtgcgc tcgccggcgg agacggtgtc
     1441 cgatctggag aagattcgcg agcttggcct gcgcggcgtg atgatgcccg gagacccgct
     1501 ggaggaagac tacgactcgc agatctacaa cccggtctgg gaagctgcca tcgacctcgg
     1561 tctgccgctt tcgtttcaca tcctcacgac ccgccagcag gctccggtca ggggtccgcg
     1621 catcaacagc tttctttcga tcatccgggg aaaccaggac atcatgggca ccctcatctt
     1681 cggcggcgtc ttcgaccgct atccggagct caaggtggtc tgcgtggagg cggacgccgg
     1741 ctgggtgccc cactacatgt accggatgga ccacgcctac aagcgtcacc gcaactggct
     1801 ggccaagggc gtcagcctgg ccaggcttcc gtccgagtac ttcagcgagc acgtctacac
     1861 cacctttcag gacgactggg tggcgttcag gctggtcaac gacatgaact ggcggcgact
     1921 gctctgggcc aacgattttc cccacagcga ctcgacctgg ccgtggtccc agcagatgct
     1981 cgcagagcag acggcgaacc tgaccgagga gcagaaacgg gggatcctct gcgacaacgt
     2041 ggcggaactc taccgcatcg atgtggacgc cctcgcccag gccgcctgat gagcatacgg
     2101 ctgaccaagc cctggtcgcc cgccagcgcc gctgggaagc tcccgggcca gatgggcgtc
     2161 tatcagctgg gcgacgaggc gggcaacgtc atcaccatcg gctacgccgg cgggcgcagc
     2221 acgttcggcc tgcgcggcga ggtcgagact gctcttcagc gtcaccctga tgccgtgagc
     2281 taccgcgttg aggtcaactc ggcctacctg tcgcgatatc gtgagctgct catggtgcac
     2341 ctggcggatt tcgggacgct gccgcccggc aacgaggccc tcccggggct cggccggctc
     2401 agcccggctt gaggggtagc catggatctc gatctgagcg aggagcagaa cctgctggtc
     2461 ggcacggtgc ggcgcttcgt tcgggaggag gtcctgcccc tggagcagtc tctggatccg
     2521 gatgcggacg agctcgagcc cgatgatcac gaacgcctga cgcagctgac ccgcgaaatg
     2581 gggctctacg gcctggacgt cccgccggag ttcggcggcc cggacgtcga tctggtgacc
     2641 cggacgctgc tcgccatgga gatgagccag catcgcgccg gtctctacgc gccgtgctac
     2701 ggggtctttg gcggtgcggg gctcgctcag ctgttcgaag cgacggagga tcagaaggat
     2761 cggtacctct tccccctgct gcgcggggag aagaagggtt tcttcggcct cactgaacct
     2821 tcgggcggca gcgatcccgc acgagcgatt cagaccaggg ccgttcgcga cggcgacgag
     2881 tgggtcgtca atggcagcaa gatcttcatc agcggtgctg accgggcgga tttcggcctc
     2941 gtgtttgcgc gcacggatgc cgacaaggga cgcaacgggg tcacctgctt catcgtggat
     3001 acggacacgc cgggctttca cgttcggcgc atcgttcaca cgctgcgcac ggcccgctac
     3061 gccaccgagc tctccttcga ggacatgagg gtggcccacg gcaacgtgct cggagaggta
     3121 aacgggggct tcgccatcgc caacgatcgg ctctccagac agcgcatacc ctatgccgcc
     3181 ggttgcatcg gcgtcgcgat caaggctcac gagatggccc tggaatacgt tccccagcgc
     3241 gagacgttcg gcgccccgct gtccacacgt caggccattc agtggatgct ggtggacaac
     3301 gagatcgaca tacgg
//
LOCUS       DBNHQR010000180         1434 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_523930, whole genome shotgun sequence.
ACCESSION   DBNHQR010000180 DBNHQR010000000
VERSION     DBNHQR010000180.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1434)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_523930"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(352..>1434)
                     /locus_tag="AC16VR_04095"
     CDS             complement(352..>1434)
                     /locus_tag="AC16VR_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HIR6010370.1"
                     /translation="DRLGHDPAMRWVVGGHATARHAASASQMGRFETEFLATDDNLAA
                     LAELSGVWIDRVHDRRPPKRVILDMDSSVSPTYGDQEGTAYNGHFGCTCYHPLFLFNH
                     MGDLERCTLRPGNVHSADGWRDVLVPVVERYKERQVRLYFRGDAAFASPEIYDYLEAE
                     GVLYAIRLPANKVLQESIAHLLHRPVGRPPNHVRRFYASFGYQAGSWSQKRRVVAKVE
                     WHPGELYPRVGFIVTNLGRPAERVVAFYNQRGTAEQWIREGKHAIRWTRLSCRRFRHN
                     EVRLQLHALAYNLANFMRTLALPRELEHWSLTTIREKLVKIGAKVVSHGRYVTFQMAE
                     VAVPRDLFRSILRLIDGLRRPCPAMA"
BASE COUNT          270 a          475 c          432 g          257 t
ORIGIN      
        1 agattgtttt ccataaccag aacacccctc tcatctcccc aaaacgacga cagagcgctt
       61 cgagccccca agctcggcac caacatcaca aggtgtgttt gagaaatacc ccctagatcc
      121 tccccagttg aagcgcccct atcctgatcc ccttagccga cattccccag atggctccca
      181 aactccatcc caatgatagc cgaacaggct ccatccagga accatgaacc gcattctggc
      241 tcagtgtacc gccctctcag cagtctgatc accctcgaca gccggtttcg gcccgattcc
      301 gcctcgtccg gacacacgtc tcccttcgtc ttcctgcctc cggcccgaag ttcaagccat
      361 cgccgggcat ggtcgtcgca ggccgtcgat cagccgcagg atgcttcgga acaaatcgcg
      421 gggcacggcg acctccgcca tctggaaggt cacgtaccgg ccatggctga cgaccttggc
      481 gccgatcttg accagcttct cgcggatcgt ggtcagcgac cagtgctcca actccctggg
      541 caacgccagg gttcgcatga agttcgcgag gttgtacgcg agcgcgtgaa gctggagccg
      601 gacctcgttg tggcggaacc tgcggcagga cagccgggtc cagcggatcg catgcttgcc
      661 ctccctgatc cactgctccg ccgtgccccg ctgattgtag aaggcgacga cccgctcggc
      721 ggggcgtccc aggttggtaa cgatgaagcc gacgcgggga tagagctcgc ccggatgcca
      781 ttcgaccttc gcaacgaccc tgcgcttttg actccacgac cccgcctgat agccgaagct
      841 ggcgtagaag cgacgaacgt gattgggcgg gcgcccgacc gggcgatgca gcagatgggc
      901 gatgctctcc tgcaggacct tgttggcagg gagccggatc gcgtagagca cgccctcggc
      961 ttcgaggtag tcgtagatct ccggtgaggc gaatgcggcg tcgccgcgaa agtagagccg
     1021 gacctgccgt tccttgtagc gttcgacgac cgggaccagg acatcccgcc agccgtcggc
     1081 actgtggacg ttgccaggac gcaatgtgca acgttccagg tcgcccatgt ggttgaacag
     1141 gaacagcggg tggtagcagg tgcagccgaa atgaccgttg taagccgtgc cctcttggtc
     1201 gccgtaggtc gggctgacgc tggaatccat gtcgaggatc accctcttgg gcggacgacg
     1261 gtcatggacc cggtcaatcc aaaccccgga cagctcggcg agggcggcga ggttgtcgtc
     1321 ggtggccagg aactcggtct cgaagcgtcc catctggctg gccgaggccg catgcctggc
     1381 ggttgcatga ccgccgacga cccagcgcat cgccggatca tggccaagac ggtc
//
LOCUS       DBNHQR010000181         1342 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1663950, whole genome shotgun sequence.
ACCESSION   DBNHQR010000181 DBNHQR010000000
VERSION     DBNHQR010000181.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1663950"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="AC16VR_04100"
     CDS             <1..769
                     /locus_tag="AC16VR_04100"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR6010371.1"
                     /translation="SSVGVKLPEGYLNVKMTNDDGEEVEQEILYPANPGSIYHMTKTQ
                     DQLFFAFYNKNDQVRVTDLHQGIVWGTQTSETRLDERLINRFDYDGDYGTVLNRFLMQ
                     AAIDYPLTVHGSGQQTRAFIHLQDTVRCVELAISNPPAAGERVKILNQLTETHRLIDL
                     ARLVSEMTGVPIEHVPNPRREADANDLAASNEGLVGLGLQPTTLKGGLLEEVTDIARR
                     YAERCDRTRIPCMSHWTTDIAGDIEVIVPSGEASTAG"
     gene            complement(766..1185)
                     /locus_tag="AC16VR_04105"
     CDS             complement(766..1185)
                     /locus_tag="AC16VR_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIR6010372.1"
                     /translation="MTYDPDNVFAKIIRGQAPAHRVYEDAWTLAFMDLMPQTDGHTLV
                     IPKSEAVSLLDVDSEALARTIQTTQKIAAAVKAAFGAHGVMVAQLSGAAAGQTVFHLH
                     FHVLPRLHGADFEIHAREVAPAEVLAEHAERIRRALG"
BASE COUNT          253 a          435 c          438 g          216 t
ORIGIN      
        1 ctccagcgtg ggcgtgaagc tgccggaagg ctatctgaac gtgaagatga ccaacgacga
       61 cggcgaggag gtggagcagg agatcctcta tccggcgaac ccgggcagca tctaccacat
      121 gaccaaaacc caggatcagc tgttctttgc cttctacaac aagaacgacc aggtgcgcgt
      181 caccgacctg caccaaggca ttgtctgggg tacccagacg agcgagaccc gcctggacga
      241 gcggctgatc aaccgctttg actacgacgg cgattacggc acggtcctca accgtttcct
      301 gatgcaggcc gccatcgact accccctcac cgttcacggc agcgggcagc agacgcgagc
      361 cttcatccac cttcaggaca cggtgcgctg cgtcgagctc gccatatcca acccgccggc
      421 cgcgggcgag cgggtgaaga ttctgaacca gctgacggaa acccaccggc tgatcgatct
      481 cgcccggctg gtctcggaga tgacgggcgt gcccatcgag cacgtgccga atccgcgtcg
      541 ggaggccgac gcaaacgatc tggccgcgag caacgaaggg ctggtggggc tcggcctgca
      601 gccgacgacg ctgaaaggcg gcctgctcga agaggtgacc gacatcgccc ggcgctatgc
      661 cgaacgctgc gaccggacgc gcatcccctg catgtcccac tggacgacgg acattgcggg
      721 ggacatcgag gtgatcgtcc ccagcggcga ggcttcgacc gccggctagc cgagcgcgcg
      781 gcgaatacgc tctgcgtgct cggcgagcac ctcggcaggc gccacctccc tggcgtgaat
      841 ttcgaagtcg gcgccatgca gcctgggcag cacgtgaaag tgaaggtgaa agaccgtctg
      901 cccggcggcc gccccgctca gctgagccac catcacccca tgggcgccaa atgccgcctt
      961 caccgccgcg gcgatcttct gggtcgtctg gatcgtccgg gcaagcgcct cggagtccac
     1021 atccagcagg ctcaccgcct cgctcttggg aatgacgagg gtgtggccgt ccgtctgcgg
     1081 catgaggtcc atgaacgcca gcgtccaggc gtcctcgtaa acccggtggg cgggcgcctg
     1141 gccgcggatg atcttggcga agacattgtc gggatcgtaa gtcatcgttg ctccggtgtt
     1201 actccggtct ccggttcaga cgtcgagcat attcgaaaag gcgcgtcatc gggcaccggt
     1261 ggtaatctgt cgttttgcgc ggggggaatg cgatgcagct agaaggccag gtcgccgtgg
     1321 tgacgggcgg cgccagcggc at
//
LOCUS       DBNHQR010000182        15962 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1347309, whole genome shotgun sequence.
ACCESSION   DBNHQR010000182 DBNHQR010000000
VERSION     DBNHQR010000182.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 15962)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15962)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15962
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1347309"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /gene="tolB"
                     /locus_tag="AC16VR_04110"
     CDS             complement(<1..1019)
                     /gene="tolB"
                     /locus_tag="AC16VR_04110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HIR6010373.1"
                     /translation="MINFARCLSALVCLVLMLTPAATLAAESLDTIIIDAGYDKQTRI
                     AVVPFRQGPEFSDRQQMGDIIGFDLVRSGQFSPLGMENMLSFPTTREEVFFRDWRVLG
                     IEYLVIGRTGVDAAGNLEVTYELFDVFNERRMTGGTTTALRSQWRDVAHRVADEVFEA
                     ITGIPGAFSTRILYVLAQNVGSPDEAFRLEVADSDGERSRTLFSSTQPLLSASWSPDG
                     TRVAYVSFEGGRPSIVLQNVATGARERLTQFSGINGSPVFSPDGKRLALVLSRDGNPE
                     IYIMDLDDRRLRRITTHHAIDTEPSWTPDGKGVIFTSDRGGRPQIYQLDLATNFIERL
                     TFQGD"
     gene            complement(1016..1735)
                     /gene="tolA"
                     /locus_tag="AC16VR_04115"
     CDS             complement(1016..1735)
                     /gene="tolA"
                     /locus_tag="AC16VR_04115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HIR6010374.1"
                     /translation="MPAVRDYALPLLLALVLHALAFLSLRAGWNPETAEVRAIKPQIV
                     RSTLVMMEPKAKPVPRPKAAAKASPLPARQLEEPKPKAEARPADPPQAPPPDVGAERR
                     RAAQAERQERLEQLAEASFLDALETEAADLAESLAAEEAEAVAQTFRYGIYERVVANW
                     SRPPSARNNMQARLLVELVPTGDVVSVTLIEGSGNEAFDRSAEAAVRKARRFEVPKDS
                     DVFERYFRRFTLLFRPEDLLR"
     gene            complement(1742..2179)
                     /gene="tolR"
                     /locus_tag="AC16VR_04120"
     CDS             complement(1742..2179)
                     /gene="tolR"
                     /locus_tag="AC16VR_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007152468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HIR6010375.1"
                     /translation="MAKRRKPMSEINVVPYIDVMLVLLVIFMATAPLLTQGVIVDLPK
                     APSEPITDRQDDPLVVSIREDGAMFVNLGMQNADDESTRVTIYSLEDQAGKILRARPE
                     VPVYVKADHKLDYGAVVRVMTVLQRVGAQSVGLITDPPELAGE"
     gene            complement(2190..2873)
                     /gene="tolQ"
                     /locus_tag="AC16VR_04125"
     CDS             complement(2190..2873)
                     /gene="tolQ"
                     /locus_tag="AC16VR_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HIR6010376.1"
                     /translation="MPEQLSIFQLIANASLLVQVVMLLLALASVASWVMIFQRWFFLR
                     RVLASIADFEEHFWSGIDLRALYNELEAQDDLTGIESIFVAGFKEYTRLSEQSGADAD
                     SIMQGAQRAMRVALNREEENLETHLAFLATVGSTSPYVGLFGTVWGIMNSFRSLASMS
                     QATLASVAPGISEALVATAMGLFAAIPAVIGYNRFSARVEVMMSRYEAFSDELTGILH
                     RAAHARGGR"
     gene            complement(2885..3934)
                     /gene="ruvB"
                     /locus_tag="AC16VR_04130"
     CDS             complement(2885..3934)
                     /gene="ruvB"
                     /locus_tag="AC16VR_04130"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003292825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HIR6010377.1"
                     /translation="MSVEPDRLVSPREDGREGGFDRALRPERLDDYVGQCAVREQMEI
                     FIQAARARDEALDHTLIFGPPGLGKTTLANIIANEMGVAIKSTSGPVLERPAELAAML
                     TNLEEGDVLFVDEIHRLSPVVEEILYPALEDFQLDILIGEGPAARSLKLELPRFTLVG
                     ATTRAGLLTSPLRDRFGIVQRLEFYTVEELTQIVSRSAAKLGLEIHPEGATEVARRSR
                     GTPRIANRLLRRVRDWAEVKADGVINRKIADAALNMLKVDAQGFDSMDRRLLSAIIEK
                     FSGGPVGLDSLAAAISEERDTIEDVLEPFLIQSGYLMRTPRGRVATEQAYLHFGLSVP
                     EASEPSGGQPRLDLR"
     gene            complement(3931..4539)
                     /gene="ruvA"
                     /locus_tag="AC16VR_04135"
     CDS             complement(3931..4539)
                     /gene="ruvA"
                     /locus_tag="AC16VR_04135"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HIR6010378.1"
                     /translation="MIGRIQGQLVEVAENVILVNAGGICYEVELTSTALGRLPGADAP
                     VDLFTHFLVREDAQLLYGFASRDERDLFRALIRITGVGPKLALALISSIPLADLARAV
                     QTNDVALLTRVPGVGRKTAERLLVELKDRLADIVVVAEAGIPARGGKSAEEAEEVLVA
                     LGYKPQEAQRVVSDVLAEGSASTEEIVRAALRHIAMRKEVSG"
     gene            complement(4536..5009)
                     /gene="ruvC"
                     /locus_tag="AC16VR_04140"
     CDS             complement(4536..5009)
                     /gene="ruvC"
                     /locus_tag="AC16VR_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HIR6010379.1"
                     /translation="MALRILGIDPGSRITGYGLVEVSGGRIRYLASGCVRPRAKAFPE
                     RLAEIYTGIRSVISEHGPEVLAIEQVFMARNPDSALKLGQARGVAIAAAVQCGLPVHE
                     YAARTVKQAVVGSGRAGKEQVQHMIRAILDLPGAPAPDAADALAIAICHVNTSRP"
     gene            complement(5017..5757)
                     /locus_tag="AC16VR_04145"
     CDS             complement(5017..5757)
                     /locus_tag="AC16VR_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007152462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR6010380.1"
                     /translation="MAGHSKWANIKHRKAAQDVKRGKLYTKIIREITVAARLGGGEVA
                     DNPRLRAAMDKALGANMPKDTIERAIARGAGGQDGSDVEELVYEGYGPAGVAILVEAM
                     TDNRNRTVAEVRHAFSKHGGNLGTDGSVAYLFNRQGVISFAPGADEEQIMEVVLEAGA
                     EDIETEGDGSVSVYTPWESLSEVVGALKAEQLEPDNAEVTMVASTYSACEPEDAEKVL
                     QLIDALEELDDVQNVYSNGDFPDSVLGA"
     gene            complement(5777..7534)
                     /gene="aspS"
                     /locus_tag="AC16VR_04150"
     CDS             complement(5777..7534)
                     /gene="aspS"
                     /locus_tag="AC16VR_04150"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HIR6010381.1"
                     /translation="MRSHYCGELNAGNVGETVELYGWVNRRRDHGGVIFLDVRDRTGI
                     AQVVFDPDAPESFAVADSVRNEYVLRLTGRVRPRPEGTVNAEMATGEVEVLGAELEVL
                     NVSETPPFQLDEHSEAGEDVRLKYRYLDLRRPEMQSRIFARARIASAVRRFLEDEGYW
                     EVETPTLTRATPEGARDYLVPSRTHPGEFFALPQSPQVFKQLLMISGMDRYFQIARCY
                     RDEDLRHDRQPEFTQIDIEASFVTAEDVMALTERMLKQVFSQALDVELPEFPVLTWAE
                     AMSRYGSDKPDLRNPLELVDVADLMRDVEFKVFNAPANDPGGRVVALRAPGGGSLSRK
                     VIDDYADFVARYGAKGLAYIKVNDLGAGRAGLQSPILKFLPDEVVSEVLARVGAGDND
                     VIFFGADTAGVVADAMGALRDALAGDLDLLEDRWAPCWVINWPMFEMGRDGVPTPHHH
                     PFTRPTCTPEEMLAEPMVAEAAAYDVVLNGYELGGGSLRIHDREMQQAVFDVLRLGEE
                     AEVRFGFLLDALKFGCPPHGGIALGLDRLVMLMTGSHAIRDVIAFPKTQTASCPMTDA
                     PGPVDEHQLRELHIRVRTN"
     gene            complement(7728..7940)
                     /locus_tag="AC16VR_04155"
                     /pseudo
     CDS             complement(7728..7940)
                     /locus_tag="AC16VR_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017139808.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
     gene            8239..8655
                     /locus_tag="AC16VR_04160"
     CDS             8239..8655
                     /locus_tag="AC16VR_04160"
                     /inference="COORDINATES: protein motif:HMM:NF012442.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR6010382.1"
                     /translation="MARKKAPAKKKAPAKKKAPAKKKAAAKAAPQRQAPALKAKMSKT
                     AMLSEIAEKTNLTRGQVGSVLDELEVLIERHVKKRSVGEFTLPGLLKIRSVKRPASKA
                     RMGRNPATGEEIMIKAKPASIRVRVTPLKKLKDMVG"
     gene            8787..10487
                     /locus_tag="AC16VR_04165"
     CDS             8787..10487
                     /locus_tag="AC16VR_04165"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014871635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HIR6010383.1"
                     /translation="MRQSMLLIPTMKENPADADVASHRLMLRAGLIRQVASGLYTWLP
                     LGLRVIRRIESIIREEMNQSGAQEVLMPVVQPAELWEESGRWQKMGPEMLRMRDRHER
                     DFCLAPTHEEVITDLFRREVHSYRQMPCNFYQIQTKFRDEIRPRFGVMRAREFTMKDG
                     YSFHLDQACFDDTYRTMYDCYSRILTRMELEFRAVEADTGNIGGANSHEFHVLAESGE
                     DTIAYATHGPYAANLDKARAAPPAAGRSPGDELRKVATPGVETIEAVARFLEVSPAQT
                     LKTLLVRGSSEPVVALILRGDHDLNELKAEKLDEVADPLSFATEAEIQAATGCRPGSL
                     GPIGLDVPAYVDVEAAALTNFVCGANEDGFHYTGANWDRDQTLDTARVVDFRNVVAGD
                     PAPDGQGTLQFLRGIEVGHIFQLGSVYSEPLGAAVLGPDGASITPIMGCYGMGVTRLV
                     AAIIEQNHDEVGIIWPDPVAPYDLHIIALNYGKSETVREAADRLYEACLSDGMEVLLD
                     DRDERPGVKFAEADLIGIPRRITVGERGLKDQTVEYRRRRDSENEVVTLDGMLDRLRS
                     "
     gene            10490..11137
                     /locus_tag="AC16VR_04170"
     CDS             10490..11137
                     /locus_tag="AC16VR_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HIR6010384.1"
                     /translation="MGRFGLSVALLLCAICPLPTPSALADVRIDPVLLEKLAVSMADR
                     YEHTDRFDAEVWLVTSERRLARYMDDHDERLALLQTVYREADRNALDADLVLAVMQVE
                     SAFDRYAISRVGAQGLMQVMPFWRNEIGRPRDNLTDMETNIRYGTTILAHYIEVSDGD
                     LVDALARYNGSRGRLKYPEKVVGAWRRVWRHKSSDELPDLVSTCATYALHACRYR"
     gene            complement(11184..11675)
                     /locus_tag="AC16VR_04175"
     CDS             complement(11184..11675)
                     /locus_tag="AC16VR_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010385.1"
                     /translation="MLRKLAIRDVLIVAVATAGWLGLLPLSEGRPLISDSLALLLGLG
                     VTVSAYLLHEWGHALAGLVVGSRIHAPASLRSVSLFSFDAEANSKTQFAAMSIGGFVV
                     TAAAMGFVYFVLPDGYMASRVARGGVTVLASITVFIEVPLLLYGLLARRLPGVVAVFR
                     PET"
     gene            complement(11678..12157)
                     /locus_tag="AC16VR_04180"
     CDS             complement(11678..12157)
                     /locus_tag="AC16VR_04180"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HIR6010386.1"
                     /translation="MKAKTSSLLLAGALFLAGCDFLDQADEPPLALADGTTMKVDDWH
                     GRWLLINYWAEWCAPCRKEIPELNRLQAEVGEERLLVIGVNFDGLTGQRLTELIEKMD
                     VRFPVLVEDPRGRWDVEQPTVLPSTLVIAPNGRLKEVLVGPHTFESFSRALGLTAST"
     gene            complement(12154..13383)
                     /locus_tag="AC16VR_04185"
     CDS             complement(12154..13383)
                     /locus_tag="AC16VR_04185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HIR6010387.1"
                     /translation="MLEATYDQFQERVVVPLRWYVGQLGQQFLIHDCMTRAGALTYTT
                     LFAVVPMMTVAYAMFSVLPEFETVGERIQEYIFQNFVPDSSAVVQERLLEFSARARQL
                     TAAGFLVLFITAFMMLVSVEKTFNTIWSVAEPRRGLQRFLLYWGVLSLGPPLIAAGIF
                     ISLYLISLPLIADLDTFGLDSAVLDYLPSVLTAAGLTVLFLAVPNCRVPFHHALAGGV
                     LTMFAFELAKKVFRLTVANSSMEPIYGTFAAVPLFLIWLYLVWVLILSGAIFVRTLSL
                     KRDRPVDNPEPAVVKCSRVVKFLHAAHLEGRSVSDSEINDQVPLARAERERVFEALLA
                     LKLVRETEDGGWMLGRSLKTLTLWDLYRALPDGVDAARLGTVKDMDGVVEPLKAFVQF
                     GSNQMAVSLDSVFGKSS"
     gene            13446..13796
                     /gene="arsC"
                     /locus_tag="AC16VR_04190"
     CDS             13446..13796
                     /gene="arsC"
                     /locus_tag="AC16VR_04190"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003252402.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HIR6010388.1"
                     /translation="MREVTIYHNPRCSKSRATLALLEEHGVTPNVVEYLSTPPDAATL
                     KRLLDLLGLSPRELMRRKETVYSDLGLDDDALGEDALIEAMIANPILIERPIVVAGDR
                     AVIGRPPENVLGLV"
     gene            13800..14408
                     /gene="wrbA"
                     /locus_tag="AC16VR_04195"
     CDS             13800..14408
                     /gene="wrbA"
                     /locus_tag="AC16VR_04195"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018403455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HIR6010389.1"
                     /translation="MTGPVHPYVLILYYSRTGGTAHLARYVARGVQAQGDFEARVRTV
                     PPVSTTVEQTEPVVPEHGAVYCTKADLAGCGALAIGSPTRFGNMAAPLKHFLDDTADL
                     WMQHALVGKPAGVFCSTASLHGGQESTLLSMMIPLLHHGMLVSGVPYSEPALNRTARG
                     GGPYGPSHVSGGEAGTEPTADEQAIAMALGRRLAELARRLGA"
     gene            14405..14755
                     /locus_tag="AC16VR_04200"
     CDS             14405..14755
                     /locus_tag="AC16VR_04200"
                     /inference="COORDINATES: protein motif:HMM:NF021369.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HIR6010390.1"
                     /translation="MKGLQLACLLMMGSLFAINGLRQFFVEPLATPHVNLIWFLIQVL
                     PLLAVLPGTLRNRYRSYVFAALVSTLYFIHGVLLAATADLRALGLWETGFAVGLLIAA
                     SYAARRTVGQAASQ"
     gene            complement(14749..15084)
                     /locus_tag="AC16VR_04205"
     CDS             complement(14749..15084)
                     /locus_tag="AC16VR_04205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HIR6010391.1"
                     /translation="MIIVHGTIPIRPDQRERALELARRMSEATQAELGCISYDFYVGL
                     SDPNTLMLFQEWETMEALMAHFQTEHMDDFLRELPQVVAGEITTRRYAVQSVDEDTEE
                     TPETPPIVH"
     gene            complement(15116..>15962)
                     /locus_tag="AC16VR_04210"
     CDS             complement(15116..>15962)
                     /locus_tag="AC16VR_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding enzyme"
                     /protein_id="HIR6010392.1"
                     /translation="ALKDRYDVSSLKYQFVAGERLDPPTYHWLSEHLHVPVIDHWWQT
                     ETGWAMCANMAGLELKDAKPGSPTLPVPGYDLRILDEDGVEVPAGVEGAVVVREPLPP
                     GAFPTVWGDHQRYVDSYWSRYPGFYLTGDEGYRDDEGYVYIMGRMDDVINVAGHRLST
                     GQMEETVAEHPAVAECAVVGIADADKGQVPVGLVLVKDGVNVDPQALEAELVQMVRAS
                     VGAFANFRRAVVVERLPKTRSGKILRQVLRKMIDGNPYSVPSTIDDPAIIPEMASTLK
                     KAGVM"
BASE COUNT         2904 a         5286 c         5045 g         2727 t
ORIGIN      
        1 tagtcaccct gaaacgtgag ccgctctatg aagttggtcg cgaggtcgag ctggtagatc
       61 tgtggcctgc cgccgcggtc cgaggtgaat atcacgccct tgccatccgg cgtccagctg
      121 ggctcggtgt cgatggcgtg gtgggtggtg atgcggcgca ggcgccgatc gtcgagatcc
      181 atgatataga tctcggggtt gccgtcccgc gagagcacca gagcgagccg cttgccatcc
      241 ggcgagaaga cgggggaacc gttgattccc gaaaactggg tcagccgctc ccgcgccccc
      301 gtggcgacgt tctgcagcac gatggacggc cgtcctccct cgaaggatac gtaggccacg
      361 cgggtcccat cgggcgacca gctggccgaa aggagtggtt gggtggacga gaacagcgtc
      421 cgcgaccgct cgccgtccga gtcggccacc tccagccgaa acgcctcgtc cggggacccg
      481 acgttctgcg cgagaacgta cagaatgcgg gtggagaacg cgccgggaat gccggtgatc
      541 gcctcgaaga cctcgtcggc gacgcgatgg gccacgtccc gccactggct gcgcagcgcc
      601 gtggtcgtgc cgcccgtcat ccgacgctcg ttgaacacgt cgaacagctc gtaggtgacc
      661 tcgagattgc cggcggcgtc cacgcccgtg cgccctatga caagatactc gatgccgagc
      721 acgcgccagt cgcggaagaa gacttcttcc ctggtggtgg gaaacgacag catgttttcc
      781 atgccaaggg gcgaaaactg gccgctgcga acaaggtcga acccgatgat gtcacccatc
      841 tgctgacgat cggaaaactc cggcccctgc cggaacggca ccacggcgat gcgcgtctgc
      901 ttgtcgtacc ccgcgtcgat gatgatggtg tccaggctct ccgcggcaag ggtcgcggcc
      961 ggcgtcagca tcaagaccag gcagacgagc gcggacaggc agcgggcaaa gttaatcaac
     1021 gcagcaggtc ctccggacgg aaaagcagtg tgaaacgacg gaagtagcgt tcgaagacgt
     1081 ccgaatcttt cggcacctcg aaacgccgtg cttttcgcac ggccgcctcg gccgagcggt
     1141 cgaatgcctc gttgccgctc ccctcgatga gcgttaccga aacgacgtcg ccagtcggca
     1201 cgagctcgac gagcaaacgg gcttgcatgt tgtttcgcgc gctgggtggc cgcgaccagt
     1261 tggcgaccac gcgttcgtag atcccgtaac ggaaggtctg cgcgacggct tcggcctcct
     1321 ccgcagccag gctctcggcg agatcggcgg cctcggtctc cagggcgtcc agaaacgagg
     1381 cttcggcgag ctgctcgaga cgctcctgcc gctcggcctg tgcggctcgt ctgcgctcgg
     1441 ccccgacgtc tggcggaggc gcctgggggg ggtccgcagg tcgcgcttcc gccttgggct
     1501 tcggctcttc gagctgccgg gcaggcaggg gtgaggcctt ggcggcggct ttcggtctcg
     1561 ggacgggttt tgccttgggc tccatcatga ccagcgtcga ccgcacgatc tgcggcttga
     1621 tggcgcgcac ctcggcggtc tcggggttcc agccggcccg gagagagaga aaggccagag
     1681 cgtgcagtac cagcgccagc agcagcggca gcgcgtagtc acggacggcc ggcattgctc
     1741 gtcactcccc ggcaagctcg ggcgggtcgg taatcagacc gacgctctgc gcgcccaccc
     1801 gctgcaggac ggtcatcacc cggaccacgg ctccgtagtc cagcttgtga tcggccttca
     1861 cgtagacggg gacctcggga cgcgcacgca ggatcttgcc ggcctggtcc tccagcgagt
     1921 agatcgtcac ccgggtgctc tcgtcgtccg cattctgcat gccgaggttg acgaacatgg
     1981 cgccgtcctc acggatggag accactaggg gatcatcctg tcggtccgtg atgggctcgg
     2041 aaggtgcttt cggcaggtcg acgatgacgc cctgcgtcag cagaggcgcg gtggccatga
     2101 agatcacgag cagcacgagc atcacgtcga tgtacggcac gacgttgatc tccgacatgg
     2161 gcttcctgcg cttggccatg aacctgcctt cacctccccc cgcgggcatg cgccgcgcgg
     2221 tggaggatgc cggtcaactc gtcgctgaac gcctcgtaac ggctcatcat gacttccacc
     2281 cgggcggaaa accggttgta accgatcacc gccggtatcg ccgcgaagag ccccatggcc
     2341 gtcgctacca gagcttccga aatgcccggc gccaccgagg cgagcgtcgc ctgactcatg
     2401 ctggccaggc ttcggaagga attcatgatg ccccacacgg tcccgaacag gccgacgtac
     2461 gggctcgtcg atcccaccgt agcgagaaag gccagatggg tttcgaggtt ttcctcttcc
     2521 cggttcaagg ccacccgcat ggcccgctgg gctccctgca tgatggaatc cgcatccgca
     2581 ccggactgct ccgacagccg ggtgtactcc ttgaagccgg ccacgaagat ggattcgata
     2641 cccgtgaggt cgtcttgggc ttccagctcg ttgtacaacg cccgcaggtc gatccccgac
     2701 cagaagtgct cctcgaagtc ggcgatggaa gccagcaccc ggcgcaggaa gaaccatctc
     2761 tgaaaaatca tgacccacga ggcgacggac gcgagcgcga gcagcagcat gaccacctga
     2821 accagcaggc tggcgttggc gatcagctga aatatggaca actgctcagg cacgatcgat
     2881 caactcacct cagatcgagg cgcggctggc caccggaggg ctccgaggct tccggcacgc
     2941 tcaggccgaa gtgcaggtac gcctgctccg tggccaccct gccgcggggg gttcgcatga
     3001 ggtaaccgct ctggatcaga aacggctcga ggacatcctc gatggtatcg cgctcttcgc
     3061 tgatggcggc cgccagggag tcgaggccca ccggcccgcc ggagaatttc tcgatgatgg
     3121 ccgagagcaa ccgtcggtcc atgctgtcga atccctgagc gtccactttc aacatgttca
     3181 gagccgcgtc cgcgatcttc cggttgatca ccccgtccgc cttgacctcc gcccagtcgc
     3241 gcacccggcg gagcagccgg ttcgcgatgc ggggcgttcc acgagatctt cgcgccacct
     3301 cggtcgcgcc ctccggatga atctccaacc cgagcttcgc cgcggatcgc gaaacgatct
     3361 gcgtgagttc ttcgaccgtg taaaactcga gtcgttgcac gatgccgaag cgatctcgaa
     3421 gcggcgacgt cagcagaccg gcgcgggtgg tcgcgccgac cagcgtgaat cgcggcagct
     3481 cgagcttgag cgaccgggcc gccgggcctt cgccgatgag gatgtcgagc tgaaaatcct
     3541 ccagcgccgg ataaaggatt tcctccacca ccgggctgag gcggtgaatc tcgtcgacga
     3601 agagcacgtc tccctcctcg agattggtca gcatggcggc aagctctgcc ggacgctcga
     3661 gcaccggccc ggaggtggac ttgatggcca cgcccatctc gttggcgatg atgtttgcca
     3721 acgtggtctt gccgaggccg ggcggcccga agatgagcgt gtgatccagc gcttcgtctc
     3781 gggcgcgggc tgcttggatg aagatctcca tctgctccct gaccgcgcac tgcccgacgt
     3841 agtcgtcgag ccgctcgggc cgcagcgcac gatcgaaccc gccctcacgg ccatcttccc
     3901 gggggctgac caaccgatcc ggctcgacgc tcacccgctc acctccttgc gcatggcaat
     3961 gtgcctcagg gcggcacgca cgatctcttc cgtgctggcg gacccttcgg ccagcacgtc
     4021 gctcacgacg cgctgcgcct cctggggttt gtagcccagc gccaccagga cctcctcggc
     4081 ttcctcagcc gacttgcctc ctcgggccgg gattccagcc tcagcgacca cgacgatgtc
     4141 ggcgagccgg tccttgagtt ccaccagaag ccgctcggcc gttttcctgc ccacgccggg
     4201 aacccgggtc agcagggcga cgtcattggt ctgcacggcg cgcgccagat cggccagcgg
     4261 aatgctcgat atcagggcca acgccagctt ggggcccacg ccggtaatcc ggatcagggc
     4321 cctgaagagg tcacgctcgt cccggctggc aaagccgtag agcagctgag cgtcctcgcg
     4381 aaccagaaag tgcgtgaaga ggtcgacggg ggcgtccgcc ccgggcaacc ggcccagcgc
     4441 ggtgctggtc aactccacct cgtagcagat accaccggcg ttgacgagga tcacgttttc
     4501 ggcgacctcg acgagctgac cttggattcg cccgatcatg ggcgggaagt attgacgtga
     4561 caaatggcaa tcgcaagcgc atccgccgcg tcgggtgccg gcgccccggg aaggtcgagg
     4621 atcgccctga tcatgtgctg gacctgctcc tttcccgcgc gccccgagcc gaccaccgcc
     4681 tgcttgaccg tgcgggccgc gtactcgtgg acgggcaacc cgcattggac ggcggcggct
     4741 atcgccacgc cgcgggcctg tcccagcttc agcgccgagt cgggattccg ggccatgaat
     4801 acctgctcga tggccagcac ctcggggccg tgctccgaga tcacgctccg gatgccggtg
     4861 tagatctccg ccagacgctc gggaaacgct ttcgcgcggg gccggacgca gccgctggcg
     4921 agatagcgga tccggccgcc ggacacctcg accaagccgt agccggtaat gcgggaaccc
     4981 ggatcgatac caaggatccg gagcgccatc ctcgcctcag gcgcccagca cgctgtccgg
     5041 aaagtcgccg ttgctgtaga cgttctgcac gtcgtccagc tcttccagcg cgtcgatgag
     5101 ctgcagcacc ttctcggcgt cctccggctc gcacgcgctg taggtcgagg ccaccatcgt
     5161 cacctcggcg ttgtccggct cgagctgctc ggccttgagc gctccgacca cctcgctcag
     5221 cgactcccag ggggtgtaga cggaaaccga gccgtctccc tcggtctcta tgtcctcggc
     5281 accggcctcg agcaccacct ccatgatctg ctcctcgtca gcgcccgggg cgaagctgat
     5341 gacgccctga cgattgaaga gatacgccac ggacccgtcc gtgcccaggt tccctccgtg
     5401 cttgctgaac gcatgtcgga cctccgcaac cgtgcggttg cggttgtcgg tcatggcctc
     5461 gacgagaatc gcgacgcccg ctgggccgta cccttcatag acgagctctt cgacgtcgct
     5521 gccatcctgc cccccggcgc cccgggcgat ggcgcgctct atggtgtcct tcggcatgtt
     5581 tgcgcccagg gctttgtcca tggcggcgcg caggcgcggg ttgtcggcca cctcgccgcc
     5641 cccgagccgg gcggctaccg tgatctcacg aatgatcttc gtgtaaagct tgccgcgctt
     5701 gacatcctgc gcggctttcc tgtgcttgat gttcgcccac ttgctgtgac cggccatgcc
     5761 cgcctccgat tgatcctcag ttcgtgcgca cgcggatgtg gagctctcgc aactgatgct
     5821 cgtctacagg tccgggcgca tcggtcatgg gacacgacgc cgtctgggtc ttgggaaacg
     5881 cgatcacgtc gcggatcgcg tgcgaaccgg tcatgagcat caccagacga tcgagcccga
     5941 gcgcgatgcc tccgtgaggc ggacacccga acttcagcgc atcgagcaga aaaccgaagc
     6001 gcacctccgc ttcctcgccg agcctgagaa cgtcgaaaac ggcctgctgc atttctcgat
     6061 catgaatccg gagggagccc ccgcccagct cgtagccgtt gagcaccacg tcgtatgcgg
     6121 cagcttcggc gaccatgggc tcggccagca tctcctcggg ggtgcacgta ggccgggtga
     6181 aaggatggtg gtgcggcgtc gggacgccgt cgcgccccat ctcgaacatg ggccagttga
     6241 tcacccagca cggcgcccat ctgtcctcca gaaggtcgag atcgccggcc agcgcgtcgc
     6301 gcagcgcgcc catggcgtcg gcaaccacgc cggcggtgtc ggcgccgaag aagatcacat
     6361 cgttgtcacc ggctccgacg cgcgcgagca cctcggacac cacctcgtcg gggaggaact
     6421 tgaggatcgg cgactgcagg ccggccctgc cggctccgag gtcgttcacc ttgatgtagg
     6481 cgagcccctt cgcgccgtag cgcgcgacga aatcggcata gtcgtcgatg accttgcggc
     6541 tgagcgagcc tccgccgggg gcccgtagcg ccaccacacg accgccaggg tcgttggccg
     6601 gagcgttgaa gaccttgaac tcgacgtcgc gcatcaggtc ggcgacgtcg acgagttcca
     6661 gcgggtttct caggtccggc ttgtcgctgc cgtaacggct catggcttcg gcccaggtga
     6721 gcacgggaaa ctcgggcagc tcgacgtcga gcgcctggct gaacacctgc ttgagcatgc
     6781 gctcggtcaa tgccatgacg tcctcggccg tgacgaacga ggcctcgatg tcgatctgcg
     6841 tgaactcggg ctgcctgtcg tggcgaagat cctcgtcccg gtaacagcgg gcgatctgga
     6901 agtagcgatc catacccgag atcatgagca gctgcttgaa cacctgcggc gactgcggaa
     6961 gcgcgaagaa ctcgcccggg tgggtacggc tgggaaccag ataatcgcgc gcgccctcgg
     7021 gcgtggcgcg cgtcagcgtc ggggtttcga cttcccagta cccttcgtcc tccaggaagc
     7081 ggcggaccgc gctggcaatg cgtgcccgcg cgaaaatgcg gctctgcatt tccgggcggc
     7141 gcaggtcgag gtagcggtac ttgagacgga cgtcctcgcc cgcttccgaa tgctcgtcga
     7201 gctgaaacgg cggggtctcg gagacgttga gcacttcgag ttccgctccg aggacctcca
     7261 cctcgccggt ggccatctcg gcattcaccg tgccctcggg tcgcggccgg acccgcccgg
     7321 tcagccggag aacgtactcg ttgcgaacgc tgtcggccac tgcgaagctc tccggggcat
     7381 ccgggtcgaa caccacctgg gcaatccccg tccggtcacg aacatcgaga aagatcacgc
     7441 cgccatgatc acggcggcga ttcacccagc cgtaaagctc taccgtctcg ccaacgttgc
     7501 cggcgttgag ttccccgcag tagtgactac gcatggctgc ttcctgctcg catgaatcca
     7561 gagcgtccct tggttccgat gggttgtgct aagacttatt ccgatttgct cgtggtcgag
     7621 cccgaggtgg tcgagctgcc gggcttggca ccgccatcgg ccggcttgct cgagctcgct
     7681 gacgacgact ccgaagtggc gcccgcgctc gacctgtccg cgctttcctt tttgtcttcg
     7741 gtgaggttgc gcttgtttcc ggacttgaaa tcggtctcgt accagccgcc acccttcaac
     7801 cggaatccgg gcgccgacac cttcttgaac agagcgtcct gcgcacactc gggacacgtc
     7861 tgcaaaggcg cttcgcttat cttctggatg acttccagct cgtggccgca gttcgagcag
     7921 cgatattcgt agatgggcat gatcgaaaat ctctgattct gcaaacgtca gttgagcgag
     7981 cggcgcatca tcttaaaaaa accagcaaaa atacagcgat atcgcaggct ttttgttgct
     8041 tggccgcttc cgatcggcta taaaccgcgg ccatcgagag tgtcggagtg acggcgagct
     8101 agccaggaat atcaggcgtt tccgtgcaag ccgcgatctc agccgccgca gtctttcggt
     8161 aaatggggac ccgctacgca accgggtatc ggagagcgaa gctggttcag ctaaataatc
     8221 cagctcaagg aatattgaat ggcacgcaaa aaggcccccg ccaagaaaaa ggctccggca
     8281 aagaagaagg cacccgcaaa gaaaaaggct gcagcgaagg ctgcaccgca gcgccaggcg
     8341 cctgctctga aggccaagat gtccaagacg gcaatgctct cggagatcgc agagaagacc
     8401 aacctgaccc gcggccaggt gggctcggtt ctcgatgagc tcgaagtcct catcgagcgc
     8461 cacgtcaaga agcgttcggt cggtgagttc actctgccgg gcctgctgaa gatccgttcc
     8521 gtcaagcgcc ccgcgagcaa agcgcgaatg ggccgcaacc cggccaccgg cgaggagatc
     8581 atgatcaagg ccaagccagc gagcatccgt gttcgggtga ccccgctgaa gaagctcaag
     8641 gacatggtcg gctagatcgt ggctccgtga ccgaacgctg gcgatgcggg cggtcatcgg
     8701 cagaaaaggg gccttgcgcc ccttttttgc tgcaatttcg ctaatctagg ccaaacgtcc
     8761 tgcaatcgat ccagagggca ctgcacatgc ggcaatccat gctgctcatt cccaccatga
     8821 aggaaaatcc ggccgacgcc gacgtggcca gtcatcgcct catgcttcgg gccggcctca
     8881 tccgacaggt cgcctccggt ctttacacct ggttgcccct ggggctgcgg gtgatccgtc
     8941 gaatcgagtc gatcattcgt gaggagatga atcaaagtgg cgcccaggag gtgctgatgc
     9001 cggtggtgca accggcagag ctgtgggagg aatccggacg ctggcagaag atgggacctg
     9061 agatgctgcg catgcgggat cgtcacgagc gcgacttctg cctggcgccc actcacgagg
     9121 aagtgatcac cgatctgttc cggcgcgagg tacacagcta ccggcagatg ccgtgcaact
     9181 tctaccagat acagaccaag ttccgtgacg agatccgccc cagattcggc gtgatgagag
     9241 cgcgggagtt caccatgaag gacggctact cgttccatct ggaccaggcc tgcttcgacg
     9301 atacgtatcg aacgatgtac gactgttact cccgtatcct gacgcgaatg gagttggagt
     9361 tccgcgccgt ggaagcagat accggcaaca tcggcggcgc gaattcccat gagtttcacg
     9421 tcctcgcgga gtcgggcgag gacaccatcg cctacgccac ccacggaccg tacgcggcca
     9481 acctggacaa ggcgcgcgcc gcgccgccgg cagccggccg ttcgccgggt gatgagctcc
     9541 ggaaggttgc caccccgggc gtcgagacca tcgaggcggt cgcccgtttt ctggaggtct
     9601 cgccggccca gaccctgaag accctgctgg tccgtggttc ttcggaaccc gtggtcgcgc
     9661 tgatcctgcg cggagaccac gatctgaacg agctcaaggc ggagaagctg gacgaggtgg
     9721 ctgacccgct cagcttcgcc acggaggctg agatacaggc agccacgggg tgcaggcccg
     9781 gatccctcgg accgatcggg ctcgacgtgc cggcctatgt ggatgtggag gcggctgccc
     9841 tgaccaactt cgtttgcggc gccaacgagg acggcttcca ctacaccggg gcgaactggg
     9901 atcgcgacca gactctggat acagcccgcg tggtggactt ccggaacgtg gtggccggcg
     9961 acccggcgcc cgacggccag ggcactctgc agttcctgcg cggtatcgag gtcggccaca
    10021 tcttccagct cggcagcgtc tacagcgagc cgctgggcgc cgcggtgctg ggaccggacg
    10081 gcgcgagcat cacacccatc atgggctgct acggaatggg cgtgacgcgc ctggtcgcgg
    10141 ccatcatcga gcaaaaccat gacgaggtcg gcattatctg gcccgatccc gtcgcaccct
    10201 acgaccttca catcattgcg ctcaattacg gcaagtcgga gacggttcgc gaagcggccg
    10261 acaggctcta cgaagcctgc ttgagtgacg gcatggaggt actcctcgat gatcgggacg
    10321 agaggccggg cgtgaaattc gccgaggcgg atctcatcgg aatcccacgg cgcatcaccg
    10381 tgggcgaacg cggactgaag gaccagaccg tcgagtatcg tcgccgccgc gactcggaga
    10441 acgaggtggt gacgcttgac gggatgctgg accggctgag gagctgatct tgggccgctt
    10501 cggcttgtct gtggcgttgc tcctgtgcgc catctgcccg ctgccgacgc catctgcgct
    10561 ggcagatgta cgcatcgacc ccgtcttgct ggaaaagctc gccgtttcca tggccgaccg
    10621 ctacgagcac acggaccgtt tcgacgcgga agtctggctg gtcacatccg agcgacgcct
    10681 cgccagatac atggacgatc acgacgagcg gctggcactg cttcagaccg tctaccgcga
    10741 agccgacaga aacgcgctgg atgcggatct ggtgcttgcc gtcatgcagg tcgagagcgc
    10801 tttcgaccgc tacgcgatct cgcgagtcgg tgctcagggg ctcatgcagg taatgccatt
    10861 ctggcgaaac gagatcggcc gaccccgtga caacctcacc gacatggaaa ccaacatccg
    10921 ctacggcacg accattctgg cgcactacat cgaggtatcg gacggcgacc tggtagacgc
    10981 tctggcacgc tacaacggca gccgcggccg cctgaagtat ccggaaaagg tcgtcggcgc
    11041 ctggcgccgc gtctggcgcc acaaatcgtc cgacgagctg cccgacctcg tatccacctg
    11101 cgcaacctac gccctgcacg cctgccgcta ccgatagggc atcgtctcga tctcgacggt
    11161 tccggcaaac aacggtccag cggtcaggtt tccgggcgga ataccgccac gacgcctggc
    11221 aggcgcctgg cgagcaatcc gtacaaaagc aggggcacct cgatgaaaac ggtgatcgag
    11281 gccagcacgg tcaccccgcc ccgagcaaca cgggaagcca tatagccgtc gggcagcaca
    11341 aaatagacaa agcccatcgc ggccgccgtg accacgaacc caccgatgga catcgcggcg
    11401 aactgggttt tgctgttggc ttcggcgtcg aagctgaaca gcgacacgct gcgcagcgag
    11461 gccggcgcgt ggatgcggct gccgaccacg agaccggcga gcgcgtgacc ccattcgtgc
    11521 aggaggtagg cactcaccgt cacgccgaga ccgagcaaca gggccagcga atcgctgatg
    11581 agcgggcgtc cttcggacaa tggcagcagg ccgagccagc cggcggtcgc caccgccacg
    11641 atcagcacgt cccggatggc gagcttgcgc aacatcgtca cgtggacgcg gttagaccca
    11701 gggcccggct gaagctctcg aaggtatgcg gccctaccag cacctccttg aggcgaccgt
    11761 tgggcgcgat gaccagcgtg ctcgggagca cggtgggctg ctccacgtcc catctgcccc
    11821 gagggtcctc tacaagcacc ggaaaccgga cgtccatctt ttcgatgagc tccgtgagcc
    11881 gctgcccggt gaggccgtcg aagttgacgc cgatcaccag tagccgctcc tcgcccacct
    11941 ccgcctgcag gcgattgagc tccggaatct ccttgcggca cggcgcgcac cactccgccc
    12001 agtagttgat cagcagccag cgcccgtgcc agtcgtcgac cttcatggtc gtaccgtcgg
    12061 ccagagccag aggcggctcg tctgcctggt cgagaaaatc acagccggcc aggaaaagcg
    12121 caccagccag caagagagag ctcgtcttcg ccttcatgag gatttaccaa acaccgaatc
    12181 gaggctcacc gccatctggt tggagccgaa ctggacgaag gccttgagcg gttcaaccac
    12241 gccatccatg tccttcaccg taccgagccg tgctgcgtcc acgccgtccg gaagcgcacg
    12301 gtagagatcc caaagcgtca gcgtcttcag gctgcgacca agcatccaac ccccatcctc
    12361 cgtttccctc accagcttga gggcgagcag cgcttcgaat acccgctccc gttcggcccg
    12421 ggccagcggc acctggtcgt tgatctccga gtcgctgacg cttcggccct cgagatgggc
    12481 agcgtggaga aacttcacga cccgcgaaca cttgaccacc gcgggctcgg ggttgtccac
    12541 gggccggtcg cgcttgagcg acagggtgcg gacgaaaatc gccccgctga ggatcagcac
    12601 ccagacgagg tagagccaga tcaggaaaag gggcaccgca gcaaaggttc cgtagatggg
    12661 ctccatgctg gagttggcaa ccgtgagcct gaataccttc ttcgccagct cgaaggcgaa
    12721 catcgtcagg acgccgccgg cgagggcatg gtggaaggga acccggcagt tgggaaccgc
    12781 gaggaagaga acggtcagcc ccgccgcggt cagcacgctc ggcaggtaat cgaggacggc
    12841 gctgtccagc ccgaaagtgt cgagatcggc gatcagagga agggatatga ggtacagaga
    12901 aatgaaaatc ccggcagcga tcagcggcgg gcccagggag agaacgcccc agtacagcag
    12961 gaatcgctgc agtccccgcc gcggctcggc tacgctccag atggtgttga acgtcttctc
    13021 cacggagacc agcatcatga aagccgtgat gaacagcacc agaaagcccg ccgcggtgag
    13081 ctggcgcgcc cttgccgaga attccaggag gcgttcctgg accacggcgc tgctgtccgg
    13141 cacgaagttc tgaaatatgt attcctggat ccgctcgccg acggtctcga actccggcag
    13201 cactgaaaac atggcgtacg ccaccgtcat catcggcacc acggcgaaga gcgtggtgta
    13261 ggtcaacgct cccgcccgcg tcatgcagtc gtggatcagg aactgctgac ccagctgccc
    13321 gacgtaccag cgcagcggca cgaccacccg ttcctggaac tgatcatacg tcgcctccaa
    13381 cattgccagt ctcccgcgcg accggttaaa tgaaatcgcc gccagaaccg aacattataa
    13441 agaccatgcg tgaagtcacc atctaccaca atccgcgctg ctccaagtcg cgggccacgc
    13501 tggccctgct cgaagagcac ggggtgacgc ccaacgtcgt cgagtacctg agcacgccgc
    13561 ccgacgcggc gaccctcaaa cggctgctcg acctgctcgg actctccccg cgagagctca
    13621 tgcgccggaa ggaaaccgtc tactccgatc tcggtctgga cgacgacgcc ctcggcgagg
    13681 atgccctcat cgaggcgatg atcgccaacc cgatactcat cgagcggccc atcgtcgtcg
    13741 ccggcgacag ggccgtcatc ggccggcctc ccgagaacgt cctggggctg gtctgaccca
    13801 tgaccggacc cgtgcacccc tacgtcctga tcctctacta cagccgtacc ggcggaaccg
    13861 cgcacctggc ccgatacgtc gcgcgcggcg ttcaggccca gggtgacttc gaggcgcggg
    13921 tgcgcaccgt gccgcccgta tccaccacgg tggagcagac cgagcccgtg gtgcccgagc
    13981 atggcgccgt ttactgcacg aaggcggacc tggccggatg cggcgccctg gccatcggct
    14041 cccccacccg attcggcaac atggcggcac cgctgaagca cttcctcgac gatacggccg
    14101 atctgtggat gcagcacgcc ctggtgggca aaccggctgg cgtgttctgc tcgaccgcct
    14161 ctctgcacgg cggccaggaa tccaccctgc tgtcgatgat gatcccgctg ctccaccacg
    14221 gcatgttggt ctccggcgtg ccttacagcg agcccgccct caaccggacc gccaggggcg
    14281 gtgggccata cggccccagc cacgtcagcg gtggcgaggc ggggaccgag ccaacggcgg
    14341 acgagcaggc aatcgccatg gccctggggc gcaggttggc cgagctcgcc cgtaggctcg
    14401 gcgcgtgaaa ggactgcagc tcgcgtgcct gctgatgatg ggcagcctct tcgcgatcaa
    14461 cgggctgcgc cagttcttcg tggagccgct ggccacgccc catgtgaacc tgatctggtt
    14521 tctgatccag gtgctgcccc tgctggccgt gcttccaggc acgctgagga accgctaccg
    14581 aagctacgtc ttcgccgcgc tggtgagcac cctttacttc atacacggcg tgctgctggc
    14641 cgccacggcc gacctcaggg cgctgggcct gtgggaaacg ggctttgccg tcggcctgct
    14701 catcgcggcg agctacgccg cccggcgcac ggtagggcaa gctgcgtctc agtgaacgat
    14761 gggcggcgtc tctggcgtct cctcggtgtc ttcgtcaacg ctctgcacgg catagcgccg
    14821 ggtcgtgatc tcccccgcca ccacttgcgg aagttctcgg aggaagtcgt ccatgtgctc
    14881 ggtctggaag tgagccatca gcgcttccat ggtctcccat tcctgaaaaa gcatcagcgt
    14941 gttgggatcg gacagcccca cgtagaagtc gtaggagatg cagccgagct ctgcctgggt
    15001 tgcctcgctc atccggcggg cgagctccag agcgcgttcg cgctgatccg gacggattgg
    15061 gatggtgccg tgaacgatga tcatctgcct gttgccgatg ttagcagcag cccggtcaca
    15121 tcacccccgc tttcttcaaa gtcgaggcca tctccgggat gatcgcagga tcgtcgatgg
    15181 tggagggaac gctgtaggga ttgccatcga tcatctttcg cagcacctgc cgcaggatct
    15241 ttcccgagcg cgtcttgggc agacgctcca cgaccaccgc ccgccggaag ttggcaaaag
    15301 cgcccaccga tgcccgaacc atctgcacga gctcggcttc aagcgcttga ggatcgacgt
    15361 tgacaccgtc cttgaccagc accagaccga cgggaacctg gcctttgtcc gcatccgcaa
    15421 tgccgaccac ggcgcactcg gccaccgcgg gatgctcggc gaccgtctcc tccatctgcc
    15481 cggtggagag ccggtggccg gctacgttga tgacgtcgtc catgcggccc atgatgtaga
    15541 cgtaaccttc gtcgtcgcga tagccctcgt cgccggtcag gtagaagccg ggatagcggg
    15601 accagtagga atcgacgtag cgctgatgat cgccccaaac cgttgggaaa gcgccgggcg
    15661 gcaggggctc cctgaccacg accgcccctt ccacgccggc tggcacctcg acgccgtctt
    15721 catcgagaat gcgcaggtcg taccccggaa cggggagggt cggcgagccc ggtttcgcgt
    15781 ccttgagctc cagaccggcc atgttcgcac acattgccca gcccgtttcc gtctgccacc
    15841 agtggtcgat cacagggacg tgcaggtgct cgctcagcca gtgataggtg ggcgggtcga
    15901 ggcgctcgcc ggcgacgaac tggtacttca gggaggagac gtcgtaacga tccttcagcg
    15961 cg
//
LOCUS       DBNHQR010000183         3416 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1727580, whole genome shotgun sequence.
ACCESSION   DBNHQR010000183 DBNHQR010000000
VERSION     DBNHQR010000183.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1727580"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1349)
                     /locus_tag="AC16VR_04215"
     CDS             complement(<1..1349)
                     /locus_tag="AC16VR_04215"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl-D-amino-acid deacylase family protein"
                     /protein_id="HIR6010393.1"
                     /translation="MSKADLVIRNATVVDGTGNPAVTGDVAVKDGRILAVGDFGGEAT
                     REIDASGLVLAPGFIDIHTHFDPQLCWDGLATPSIEHGITTVVIGNCSLSLAPVKGDG
                     RDKIVNMFGVIEDIKKPTFDAAVPFSWESFEEYLDFIRPNLGINVAALIGHSALRFYV
                     MGAQSQERAATADEIEEMCRLVEEAMAAGAAGISSSYVDIDENMNPVPSRLADLDEKI
                     ALAKAMAKSGRGIWQVVPYFPDMQQQLANIRELGEISLAAGVPCSIQPVLSSPTDPTA
                     SEILTALEAERERGARVYGQVMPRCFDMNMRLSETSMLLYALPKWKAIMDMPRSERRA
                     RFEDPAMRTELVEEMRNAQGMSMALPMLTVGRVQAEANQPYQGRMLAEIAQAEGKDLG
                     EVILDLSLADDLDAEFRLQNVLNADKAAVADLIDHPLLHFGASDAGAHITQFCGTGDT
                     "
     gene            complement(1399..2178)
                     /locus_tag="AC16VR_04220"
     CDS             complement(1399..2178)
                     /locus_tag="AC16VR_04220"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6010394.1"
                     /translation="MANILDQFKLDGQVAVVTGGGRGIGEGIALGMAEAGADIVVAAR
                     RTDEIEAVAEKVRALGRRALAVTTDMMQIEQVQALAQRAFEEMGKLTCWVSNAGGADD
                     RVPRTLLEMPERQWDFQLNLNLKAVWTGAQAAANIMKDHGGGTIINISSTAANRASPF
                     NGPYGVAKSGVNNLTQTLGAELAQFGIRVNGVSPGPIPTEVFMEFFGITEDDIPKLGE
                     RFEIPLGRVGTPEDIATAVIFLASRASSWMTGETFLVNGGR"
     gene            2281..3315
                     /locus_tag="AC16VR_04225"
     CDS             2281..3315
                     /locus_tag="AC16VR_04225"
                     /inference="COORDINATES: protein motif:HMM:NF012472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6010395.1"
                     /translation="MLDRTDRRRFVRQLGLLGVAGQTLLRSGSLRAAVGEARDSAALD
                     SAWPEMAYRRLGRTGFEGSRLIFGCGAALMDRPRDDLLERAFEAGVNVFDSGTSRYYD
                     NAEKNLAPFLKRHRDEIFLISKAMVYLDVAPDEQITVAQARQAAQTWTGLMDESLKEL
                     EVDRIDAYYLMASNNPTVVGSEEINEAFQRARQAGKVRYLGLSTHQNAENVLKTAIET
                     GWYDLAMIAITPGGWYDWENRSVLEGSPTMVELQPLLAKASEAGIGLIGMKAGRFLAG
                     RKWLGWGNPEAFDGHYDQSFLRAQLSAFQRSYAYVLAHGLDAVNADMQNFAHLKENFV
                     AAATSHRYFA"
BASE COUNT          579 a         1095 c         1121 g          621 t
ORIGIN      
        1 gtgtcgcccg ttccgcagaa ctgggtgatg tgggcgccgg cgtcggaggc gccgaagtgc
       61 agcagcggat ggtcgatgag gtcggcgacg gctgccttgt cggcgttgag cacgttctgc
      121 aagcggaact ccgcgtccag atcgtctgcc agggacagat ccaggatcac ctcgccgaga
      181 tccttgccct cggcttgcgc gatctctgcc agcatcctgc cttgataagg ctggttggcc
      241 tcggcctgca cccggcccac ggtgagcatc ggcaaggcca tgctcatgcc ttgggcgttg
      301 cgcatctcct ccaccagctc ggtacgcatg gcgggatcct cgaaacgtgc gcggcgctcg
      361 ctgcgaggca tgtccatgat ggccttccac ttgggcaggg catacagcag catgctcgtc
      421 tctgaaagac gcatgttcat gtcgaagcag cggggcatga cctggccgta gacgcgggcg
      481 ccgcgctccc gttccgcctc cagcgcagtg aggatctcgc tggccgtggg gtccgtcggc
      541 gaggaaagca ccggctgaat ggaacagggc acgccggcgg ccaggctgat ctcgccgagc
      601 tctcgaatgt tggccagctg ctgctgcatg tcggggaagt agggcaccac ctgccagatg
      661 ccccgaccgc ttttggccat ggccttggcg agcgcaatct tctcgtccag atcggccagc
      721 cggctgggga ccgggttcat gttctcatcg atgtccacgt aagacgagga gatgccggcc
      781 gcgccggcgg ccatggcctc ttccaccagc cggcacatct cctcgatctc gtcggcggtt
      841 gccgcccgct cctggctctg tgcgcccatc acgtagaagc gcagggcgga gtggccgatg
      901 agcgccgcca cgttgatgcc gagattgggc cggatgaagt ccaggtactc ctcgaagctc
      961 tcccagctga acggcaccgc cgcgtcgaat gttggcttct tgatgtcctc gatcaccccg
     1021 aacatgttga cgatcttgtc gcggccgtcg cccttgacgg gggcgaggct taacgagcag
     1081 ttgccgatga ccacggtggt gatgccgtgc tcgatggatg gcgtggcgag gccatcccag
     1141 caaagctgcg ggtcgaagtg ggtgtggatg tcgatgaacc cgggagccag aaccaggccg
     1201 gaggcgtcga tctcgcgggt ggcctcgccc ccgaagtcgc caacggcgag tatcctgccg
     1261 tccttcacgg caacgtcccc ggtgactgcc gggttgccgg tgccgtctac gacggtagcg
     1321 ttgcgtatga ccagatcagc tttgctcatc cgaatttcct catgattagc gatcgctcag
     1381 tcgaccgggc tccttggatc aacggccgcc gttcaccagg aaagtttctc ccgtcatcca
     1441 ggaggaggca cgggacgcca gaaagatcac ggcggtggcg atgtcttcag gggtgcctac
     1501 gcggccgagc ggtatctcga aacgctcgcc cagtttcggg atgtcgtctt cggtgatgcc
     1561 gaagaactcc atgaacacct cggtggggat ggggcctggc gagaccccgt tgacgcggat
     1621 gccgaactgc gcgagctccg cgccgagggt ctgggtgaga ttgttcacgc ccgacttcgc
     1681 gaccccatag gggccgttga agggcgaggc gcggttggcg gccgtggacg agatgttgat
     1741 gatcgtcccg ccgccgtggt ccttcatgat gttcgctgcg gcctgggcac cggtccagac
     1801 tgccttgagg ttcaggttca gctgaaagtc ccattgccgt tccggcatct ccaaaagcgt
     1861 gcgcggcacc cggtcgtcgg cgccgcccgc gttgctgacc cagcaggtca gcttgcccat
     1921 ctcctcgaac gcacgctgag ccagagcctg aacctgctcg atctgcatca tgtccgtggt
     1981 gaccgccaga gcccgtcgac caagagccct gaccttctcg gccaccgctt cgatctcatc
     2041 ggtgcgacgt gccgcgacca cgatgtccgc acccgcttcc gccatgccca gcgcgatgcc
     2101 ttcgccgatg cctcgtccgc cgccggttac caccgcaacc tgaccgtcta gcttgaactg
     2161 gtcgagtatg ttcgccattg ccccttgccc ccgggaaaag tcaaagcgca gcagtatacc
     2221 caaattgtcc gggcgatcgc ctagaatgag cggatcgcta atcggcaggg gagccgtgac
     2281 atgctcgacc gcactgaccg tcggagattc gttcgccagc ttgggctgct tggcgtggct
     2341 ggccagacgc tgctgcgctc ggggagcttg cgcgcggcgg tgggcgaagc gcgcgattcg
     2401 gccgcgctcg actcggcctg gccggagatg gcctaccgca ggctcggccg cacgggcttc
     2461 gaggggagcc gcctcatatt cggctgcggg gcggcgctca tggaccgccc ccgggacgac
     2521 ctgctcgagc gcgccttcga ggcgggcgtg aacgtgttcg actcgggaac gtcgcgctac
     2581 tacgacaacg cggagaagaa cctggcccct tttctgaagc ggcatcgaga cgagatcttt
     2641 ctcatctcaa aggccatggt ctacctcgac gtggcgcccg acgagcagat taccgtggcc
     2701 caggcgcgtc aggccgcaca gacctggacg ggcctgatgg acgaaagcct gaaggagctg
     2761 gaggtggacc gcatcgacgc ctattacctc atggcgtcca acaacccgac cgtggtcggc
     2821 agcgaggaga tcaacgaggc gtttcagcgc gcgcggcagg cgggcaaggt gcgctacctg
     2881 ggtttgagca cccatcagaa cgcggaaaac gtgctgaaaa cggccatcga aacgggctgg
     2941 tacgacctgg ccatgatcgc catcaccccc ggcggctggt atgactggga gaatcgctcg
     3001 gtgctggaag gctcgcccac catggtggag ctccaacctc ttcttgccaa agcgagcgaa
     3061 gcgggcatcg ggctcatcgg catgaaggcc gggcgctttc tggccggccg gaaatggctc
     3121 ggctggggca accccgaggc gttcgacggg cactacgatc agagtttctt gagagcccag
     3181 ctgtccgctt tccagcgcag ctacgcttac gtgctggccc acggcctcga tgcggtgaac
     3241 gcagacatgc agaacttcgc gcacctcaaa gagaacttcg tggccgcggc cacctctcat
     3301 cgctacttcg cctgatggcc gacgtcaatc gacaggtcgt gctggcgtcg cggcccgacg
     3361 gctatccggt cgacagtgac ttccgcgtgg aggaggcagg ggtacccgac gtcggg
//
LOCUS       DBNHQR010000184         4328 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_968712, whole genome shotgun sequence.
ACCESSION   DBNHQR010000184 DBNHQR010000000
VERSION     DBNHQR010000184.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4328)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4328)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4328
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_968712"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="AC16VR_04230"
     CDS             complement(<1..392)
                     /locus_tag="AC16VR_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010396.1"
                     /translation="MSKDYIISWKVAGVALGLLLTLATALVQPLGVSTQFVVTDAMVA
                     HKVAPQFAESNEYLAKYGEKTDWGVGYGWMLFLGMLVGGAVTHLVLRNRQPEADKGSM
                     PAMWVARFGDSHAKRYAAAFFGGVLLLFG"
     gene            503..964
                     /locus_tag="AC16VR_04235"
     CDS             503..964
                     /locus_tag="AC16VR_04235"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR6010397.1"
                     /translation="MLFALTLAAVTSLLSPADDLQHTAARAERLGLPILLYVSRSDCS
                     FCRRFENDVLFPLIRSGEIDGKILIRELEWDRSAPIADFTGTRISAATLAENYDAKLT
                     PTLLFLDHEGKELISRMTGYHESDYASYYLEAAVDLAFQRMSATIQGETGK"
     gene            961..2205
                     /locus_tag="AC16VR_04240"
     CDS             961..2205
                     /locus_tag="AC16VR_04240"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004308 - exo-alpha-sialidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HIR6010398.1"
                     /translation="MSLTDQSGLRVRPICLVVLLAFLAACQESDRTQIAVRDLPVPAG
                     ASSQLPHLSKSEDGTVVMSWVEQRHATATLRHARLSDAGWTLPVTVASGDRWFVNWAD
                     FPSVVPIRGRIWAAHWLTKSRGGYYAYDVAMATSRDAGATWSSPSTPHQDGTATEHGF
                     VTLFPWNGDVGVVWLDGRRTAEPPSGSAASKGMTLRGARIGPDGDARDGELIDDLVCD
                     CCQTDAAMGPDGPFVVYRNRTTDEIRDIRLARFVDEAWEADISVGEDGWEIAGCPVNG
                     PAIDVVGNSVAVAWYTEAQGRARVRLSRSRDGGAGFGPPIDVSADRPIGRVDVVQSPD
                     GETFVSWLRRGDDDSAELRLSRLAPDGSVAMETVVARTSAARPSGFPQMISDAHSLIF
                     AWTEPGEDDSRIRSARVELTER"
     gene            complement(2208..3095)
                     /locus_tag="AC16VR_04245"
     CDS             complement(2208..3095)
                     /locus_tag="AC16VR_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010399.1"
                     /translation="MARGAVIRLVLGIISVAIAVACDRTMGTARDSDAAVPSGSFSLP
                     ATEAVAETITEPARLVELFAALEETGLRVHHVDHEGEAAMLLVEVDEQHFEPVLVGAD
                     GRSRTGVTAVEALTDFGLDVVIGSSFVSELHSLTPVGLLQIEGTVISELQAHGYTRIL
                     GIRPAGLGVVPIRAYHRGMFDSAVQAGPGVVEEGRLDISERDLTRPKYYRTFVGLCNG
                     KALLGASQVPMNLHTVGKRLLEYLGETGRTCDEVVNLAGDREVVLALRSADKRRVAFF
                     GHPLTAKAGLLGFRRRPKD"
     gene            3251..>4328
                     /locus_tag="AC16VR_04250"
     CDS             3251..>4328
                     /locus_tag="AC16VR_04250"
                     /inference="COORDINATES: protein motif:HMM:NF012398.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR6010400.1"
                     /translation="MGLDVEIPGLTQQFIGGVWRDSSPDTLVDVISPSTEEVLTRVAA
                     PTVADADRAVAEARAAFDMGPWALMSVEERVEVCARLCDALEARLDQLNRAWAFESGA
                     TIAHGEMINSGAGVAIWRNALEAAASLPWQEDRGDTLIVREPIGTVLGILTFNGPIVL
                     MGMKIIPALLAGCTVIVKHAPESPLTSRLIAEAIREADFPEGVLSVLAADTPVTQHLV
                     GHPDVDMVALTGGTAIGVDVVKRTADRLARTALELGGKSPAIIAEDVDLEDVMATLVD
                     GASGFMGQVCVSLSRILAPRSRYGEVVDAFAERYAALRVGDPFDPATDRGPLAVERAR
                     ERTERYVAGAHQEGARIVTGGRRPP"
BASE COUNT          736 a         1456 c         1414 g          722 t
ORIGIN      
        1 ccgaacaaca gcagcacgcc gccgaagaag gccgccgcgt agcgcttcgc gtgggaatca
       61 ccgaaccggg cgacccacat ggccggcatc gagcctttat ccgcttccgg ctggcgattg
      121 cggagaacga gatgagtgac cgcgccccca accagcatgc ccaggaacag catccagccg
      181 tagccgacgc cccaatccgt cttctcgccg tacttcgcga ggtactcgtt ggattcggcg
      241 aattgcggcg cgactttgtg cgctaccatg gcgtccgtca cgacgaactg ggtagaaaca
      301 ccgagcggct ggacgagggc ggtggcaagg gtcagcagca gacccagcgc gaccccggcg
      361 actttccagg aaatgatgta atccttgctc aacggaggaa cctccacggt gaacgatgac
      421 aggaacgtac attatcattt tcgtatatta atgaaaactg ataaaaggcc ccctcttccg
      481 gggcggactc aggagtagac gtttgctttt cgcactcacc ctggcggccg tgacgagtct
      541 cctgtctccc gccgacgacc tccagcatac ggctgcccga gccgaacggc tcggcctgcc
      601 gattctcctg tacgtgtccc gaagcgactg cagtttctgc cggcgcttcg agaacgacgt
      661 gctgtttccc ctgatccgct cgggcgagat cgatgggaaa attctcatcc gggagctgga
      721 gtgggaccgc agcgcgccaa tcgccgactt caccggcaca cggatctctg ccgcgacgtt
      781 ggctgaaaac tacgatgcaa agctcacccc gaccctcctg tttctcgacc atgagggtaa
      841 agagctcatc tcacgaatga ccggctacca tgagtcggac tacgcctcct actatctgga
      901 ggccgccgtg gacctcgctt tccagcggat gagcgcgacc atacaggggg aaaccggaaa
      961 atgagcctga ctgaccaatc cggccttcga gttcgcccga tctgcctcgt cgtcctgctg
     1021 gccttcctcg cagcctgcca ggaatccgac cggacgcaga tcgccgtacg ggatctcccg
     1081 gtgccggcgg gggcgagcag ccagctgccc cacctttcga aaagcgagga cggcaccgtg
     1141 gtgatgagct gggtcgagca acgacacgcc acagccacgc tgcgccatgc gcggctgtcc
     1201 gatgcgggct ggaccctgcc ggtcaccgtg gccagcggtg accgatggtt cgtcaactgg
     1261 gccgacttcc catccgtcgt tcccatccgt ggcaggatct gggccgccca ttggctgacg
     1321 aagagccggg gcgggtacta cgcctatgac gtcgcgatgg cgacgtcgcg tgacgcgggc
     1381 gccacctggt cgtcgcccag cacgccccat caggacggga ccgcgaccga gcacggcttc
     1441 gtgaccctgt tcccgtggaa tggcgacgtg ggcgtggtct ggctcgacgg ccgccggacg
     1501 gccgaacccc cgtcgggcag cgcagcatcg aaggggatga ccctgcgcgg cgctcgcatc
     1561 ggtcccgatg gcgacgcccg ggacggcgag ctgatcgacg acctcgtctg cgattgctgc
     1621 cagacggacg cggccatggg tccggatggt cctttcgtgg tctaccggaa ccggacgacg
     1681 gacgagatcc gggacattcg gctggcccgc tttgtcgatg aggcatggga ggcggatatc
     1741 tccgttggcg aggacgggtg ggagatcgcc ggctgcccgg tcaacggccc cgccatcgat
     1801 gttgtcggca actctgtggc cgtcgcctgg tacacggagg cccagggccg ggcgcgcgtc
     1861 cggctgagcc ggtcgaggga cggcggcgcg ggcttcggcc cgccaatcga cgtgagcgcc
     1921 gaccgcccca tcgggcgcgt ggacgtggtt cagtccccgg acggcgaaac gtttgtcagc
     1981 tggctgcgtc gcggggacga cgattccgcc gagctgcgtt tgagccgcct cgcgcctgac
     2041 ggctccgtcg cgatggagac ggtcgtcgcc cgaacgtcgg cggcccggcc ctcggggttc
     2101 ccacagatga tctccgacgc ccacagcctg attttcgcgt ggaccgaacc gggcgaggat
     2161 gattcgcgca ttcgctccgc ccgggtcgag ctcaccgagc gctgatttca gtccttcggg
     2221 cggcggcgaa aaccgagcaa tccggccttg gccgtcagcg gatgaccgaa aaaggcgacc
     2281 cgacgcttat cggccgaccg cagcgccaga accacctcgc gatccccggc caggttgacc
     2341 acctcgtcac acgtccgtcc cgtttccccg aggtattcga gcagccgttt gccaacggta
     2401 tggaggttca tggggacctg gctggcgccc agcagggcct taccgttgca aaggcccacg
     2461 aaggtccggt agtacttggg tctggtcagg tcccgctcgg agatgtcgag cctgccctcc
     2521 tccacgaccc caggaccggc ttgcacggcg gagtcgaaca tgccgcggtg ataggcccgg
     2581 atcggaacca caccgagacc ggccggccgg atgcccagaa ttcgggtgta accatgggcc
     2641 tgcagctcgc tgatcacggt gccttcgatc tggagcaggc ccaccggggt gagggagtgc
     2701 agctcggaga cgaagctgct gccgatgacg acgtccagcc cgaaatcggt gagcgcctcc
     2761 acggcggtta cacccgttcg gctgcgcccg tcggcgccaa ccagaacggg ctcgaagtgc
     2821 tgctcgtcca cctcgacaag cagcatggcg gcttcgccct catgatcgac gtggtggacc
     2881 cgcaacccgg tttcctcgag cgccgcgaac agctcgacga gacgtgcagg ttcggtgatg
     2941 gtctccgcca ccgcttccgt cgcgggcagc gaaaagcttc cgctgggcac cgcagcgtcg
     3001 ctgtcccgcg ccgtgcccat cgtccgatca caggcaacgg caatcgccac cgagatgatg
     3061 ccgaggacga ggcggatcac ggccccgcgg gccaagtcga ttggggcgcc cctgctcacc
     3121 tgatctgatt ccccgtattg catgccgaca ggccgccatt atggcatcac ccgggcacgc
     3181 cgcgcggccc gctgtggcga tcgacgcgcc tttgtcgcaa actaactttt tccatttgag
     3241 ggaacgggcc atggggctag acgtggaaat tccagggctg acacagcagt tcatcggcgg
     3301 cgtctggcgc gattcttcac cggacacgct cgtggacgtc atatctcctt caaccgagga
     3361 ggtgctcaca cgggtggctg cgccgacggt ggcagacgcc gaccgggctg tcgccgaagc
     3421 gcgagcggcg ttcgacatgg gaccgtgggc cctgatgtca gttgaggaac gcgtcgaggt
     3481 ctgtgcacgg ctttgcgacg ccctcgaggc ccgtctggat cagctgaatc gcgcctgggc
     3541 attcgagtcg ggcgccacta tcgctcacgg cgagatgatc aactccggcg ccggcgtggc
     3601 catctggcgc aacgcgctgg aagcggccgc gtcacttccg tggcaggaag accgggggga
     3661 tacgctcatc gtccgggagc ccatcggcac ggttctcggg attctgacgt tcaacgggcc
     3721 catcgtgctg atgggcatga agatcattcc cgccctgctg gccgggtgca cggtgatcgt
     3781 gaaacacgct cccgagtcgc ccctgacttc caggctcatc gccgaggcca tccgtgaagc
     3841 ggactttccg gagggcgtgc tcagcgtcct ggccgccgac acgccggtca ctcagcatct
     3901 ggtgggccat cccgatgtcg acatggtggc cctgaccggc ggcacggcca tcggcgtaga
     3961 tgtggtcaaa cgcaccgccg acaggctggc ccgcaccgcg ctggagctcg gtggcaagtc
     4021 gccggccatc atcgccgagg acgtcgacct ggaggacgtc atggcgaccc tcgtcgacgg
     4081 cgcgtccggc ttcatgggcc aggtttgcgt gagcctctcg cgcatactcg cgccgcgcag
     4141 tcgttacggc gaggtggtcg atgcctttgc cgagcgctac gctgccttgc gcgtgggcga
     4201 ccccttcgat cccgccacag accgcggccc actggccgtg gagcgggccc gggaacgcac
     4261 cgaacgctac gtcgccggcg cgcatcagga aggcgcccgg atcgtcaccg gcggtcggcg
     4321 gccccccc
//
LOCUS       DBNHQR010000185         2431 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_968898, whole genome shotgun sequence.
ACCESSION   DBNHQR010000185 DBNHQR010000000
VERSION     DBNHQR010000185.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_968898"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1323
                     /locus_tag="AC16VR_04255"
     CDS             <1..1323
                     /locus_tag="AC16VR_04255"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HIR6010401.1"
                     /translation="PAAAAAGISLGSTLATAHSIEPRLRHCNRDEIRERERLELLAGA
                     LYGFSSHVSTQPPSSLVLEAGGSLELFGDLAKLEQAVRETCEALGHVTITRKARTPSA
                     AMTLARARQDDLKHVPLELTELNAATVERFANMGMHALGALIALPEDELAHRFGPELV
                     NYLKRLTGRMPDPREAVTPAEAFSSRLHLLEAVTGKDALLFPMQRLLGELQIWLVSRQ
                     LGAEQIVWTLDDHGSKDTAELTIRFARAQQRREAFLDITRLKLESAELPGDVLGIGLR
                     AERLVPWQAGSRTLFQSQPGQAPADAAGLVDQLCARLGNRACHGIRPVDQHAPEQAWR
                     RSRPAMNGRGGRRSRSRSGKHGNGRATDGEEALLPAPDERPIWLLSRPRPINRSELKL
                     LKGPERIRTAWWQRGISRDYYVARDRRGAECWAFVDAGNNWYLHGYFA"
     gene            1307..>2431
                     /locus_tag="AC16VR_04260"
     CDS             1307..>2431
                     /locus_tag="AC16VR_04260"
                     /inference="COORDINATES: protein motif:HMM:NF014826.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR6010402.1"
                     /translation="MATSPDKPYDGGFAELHCISNYSFLRGASHPEELVKRATELGYR
                     ALALTDECTYAGLVKAHVAARELGIKLIVGAEFRLEEGIHLVLLAPDRSAYGQLSALI
                     TKLRRRSPKGAYEVCLDDFRWGVDECLALWVAGDGDIETLMAQGRRLSERWRELWIAL
                     ELFREDKDLDKMANALTLSMRLDLPIVASNDVHAHLPARQPLQDVLTAIRLKTTVAEL
                     GCAAHRNGERHLRSLSELVSLYPPEMLEASCQIADRCRFSMDELRYEYPEELVPPHLT
                     PGEYLRQLTLAGAAERWPDGVPADTLALIERELTLIREMRYEYYFLTVQDIVQFARSQ
                     QILCQGRGSAANSAVCYALGITAIDPVRMGLLFERFMSKER"
BASE COUNT          399 a          865 c          801 g          366 t
ORIGIN      
        1 ccggccgcgg ccgccgccgg catctcccta ggcagcaccc tggctaccgc tcacagcatc
       61 gagccgcggc tgcggcactg caaccgggac gagatccggg aacgggagcg gctggagctc
      121 ctggccggag cgctctacgg tttctcctct cacgtctcca cccagccgcc gagctccctg
      181 gtgctggagg caggcggcag cctggagctg ttcggcgatc tggccaaact ggagcaggca
      241 gtccgcgaga cctgcgaagc tctcggtcac gtcacgatca cgcgaaaggc ccgaaccccc
      301 tcggcggcca tgaccttagc ccgtgcccga caggacgatc tgaaacacgt ccccctggag
      361 ctcacggagc tgaacgcggc gacggtggag cgcttcgcca acatgggcat gcacgccctg
      421 ggagcgctga tcgccctgcc ggaagacgag ctcgctcatc gcttcggccc ggagctcgtg
      481 aactatctca aacggctgac cggccggatg ccggatcccc gggaggccgt gacgccggca
      541 gaggcgttct ctagccggct gcacctgctg gaggcggtga cgggcaagga cgccctgctc
      601 ttccccatgc agcggctgct cggcgagctg cagatctggc tggtgagccg gcagctgggt
      661 gccgagcaga tcgtctggac gctcgacgat cacggatcga aggacacggc cgagctgaca
      721 atccgctttg ccagggccca gcagcggcga gaggcctttc tcgacatcac ccggctgaag
      781 ctcgaaagcg cggaattgcc cggcgacgtg ctgggcatcg gcctgcgtgc cgagcgcctg
      841 gtgccctggc aggccggcag ccggacactt tttcaatccc agcccgggca ggcccccgcg
      901 gacgctgccg ggctcgtcga ccagctttgc gcccggctgg gcaaccgggc ctgccacggc
      961 atccgcccgg tggatcagca cgcgcccgag caggcctggc gtcgttcacg gcctgccatg
     1021 aacgggcgcg ggggcaggcg cagtcgcagt cgcagcggca agcatggcaa cggcagggcg
     1081 acggacgggg aagaagcgct cctgcccgcg cccgacgagc ggccgatctg gctgctcagc
     1141 cggccgcgcc cgatcaacag gagcgagctg aagctgctca agggccccga gcgtatccgc
     1201 actgcctggt ggcagcgcgg catcagccgg gattactacg tcgcccggga ccgccggggc
     1261 gctgaatgct gggccttcgt cgacgcgggg aacaactggt acctgcatgg ctacttcgcc
     1321 tgacaagccg tacgacggtg gcttcgcgga gctgcactgc atcagcaact acagctttct
     1381 gcgcggagcc tcccatccgg aggagctcgt caaacgggcc accgagctcg gctaccgcgc
     1441 gcttgcgctg accgacgagt gcacctatgc gggactcgtc aaggctcacg tagcggcgcg
     1501 cgagctcggc atcaagctca tcgtgggcgc ggagttccgc ctcgaggaag gcattcatct
     1561 ggttctgctg gcgccggacc gcagcgccta cgggcagctc tccgcgctga tcaccaagct
     1621 gcgcagacgc tcccccaagg gcgcctacga ggtctgcctg gacgacttcc gctggggcgt
     1681 ggatgaatgc cttgcgctgt gggtagccgg cgacggcgac atcgaaaccc tcatggccca
     1741 gggcagacgg ctcagcgaac gctggcgcga gctctggatt gctctggaac tcttccggga
     1801 ggataaggac ctcgacaaga tggccaacgc cctgaccctg tccatgcgcc tggatctgcc
     1861 catcgtggcc agcaacgacg tccacgcgca cctccccgcc cggcagcccc tgcaggacgt
     1921 cctcaccgcc attcgcctga agaccacggt cgccgagctc ggctgcgcgg cccatcgcaa
     1981 cggcgagcgc cacctgcgct ccctgagcga gctcgtctct ctctacccac cggaaatgct
     2041 cgaggcgtcc tgccagatcg ccgaccgctg ccgcttctcc atggacgagc ttcgctacga
     2101 gtatcccgaa gagctggtgc ccccccacct gactccgggt gagtacctgc ggcagctcac
     2161 ccttgccggt gcggccgaac gctggccgga cggcgtgccc gcagacaccc tcgcgctcat
     2221 cgagcgggag ctcacactca tccgcgagat gcgctacgag tactacttcc tcacggtgca
     2281 ggacatcgtg cagtttgccc gcagccagca gatcctctgc cagggccgcg gctcggcggc
     2341 caactcggcc gtgtgctacg cgctggggat caccgcgatc gacccggtgc ggatggggct
     2401 gctcttcgag cgcttcatgt ccaaggagcg g
//
LOCUS       DBNHQR010000186         2289 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_525222, whole genome shotgun sequence.
ACCESSION   DBNHQR010000186 DBNHQR010000000
VERSION     DBNHQR010000186.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_525222"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1512)
                     /gene="ftsH"
                     /locus_tag="AC16VR_04265"
     CDS             complement(<1..1512)
                     /gene="ftsH"
                     /locus_tag="AC16VR_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR6010403.1"
                     /translation="MGKNLLLWLIIAAVLLTVFNNFNVKPEPESLSYSAFIDQVRNDQ
                     VDKVEIEGLIIRGVRDSGEEFQVTRPDLYDPKLLDDLYNHDVQIMGKLPEQQSIWAQL
                     LIASFPILIIIAVFMLFMRQMQGGAGGRGGPMAFGKSKARLLSEDQIKTTFADVAGVD
                     EAKEDVQELVEFLRDPGKFQRLGGRIPRGILMVGQPGTGKTLLAKAIAGEAKVPFFSI
                     SGSDFVEMFVGVGASRVRDMFEQAKKQAPCIIFIDEIDAVGRHRGAGLGGGHDEREQT
                     LNQLLVEMDGFEPNDGIIVIAATNRPDVLDPALLRPGRFDRQVVVSLPDIRGREQILK
                     VHMRKVPLADDVDPAIIARGSPGFSGADLANLVNEAALFAARGGKRLVEMEEFDKAKD
                     KIMMGAERKSMVMSEKEKRNTAYHEAGHAIVGRLMPDHDPVYKVSIIPRGRALGVTMF
                     LPEEDRYSLSRQAVISQICSLFGGRIAEELILGPEFVTTGASNDIERATALARN"
     gene            complement(1636..2175)
                     /locus_tag="AC16VR_04270"
     CDS             complement(1636..2175)
                     /locus_tag="AC16VR_04270"
                     /inference="COORDINATES: protein motif:HMM:NF013856.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR6010404.1"
                     /translation="MSRAHFKLDQLDRRFRLISPGMWVLELGAAPGGWTAYLESRLGP
                     SGRLIVCDSRPVHAGADTVVVSGLFGDAVVDEAIAGALGDRGVDLVLSDMSPNITGIR
                     TRDQAETLDLAELAMAAAERWLKPGGVLVVKMFMGEGTDAWIRQIGKKFAKIRRAKPD
                     ASRQESREIYVVGQQFLAQ"
BASE COUNT          448 a          756 c          647 g          438 t
ORIGIN      
        1 gttgcgggcc agcgcagtgg cacgttcgat gtcgttggac gcgccggtgg tcacaaattc
       61 cggcccgaga atgagctctt cggctatccg cccaccgaac aggctgcaaa tctggctgat
      121 gaccgcctgc cgactcagac tgtagcggtc ttcctcggga agaaacatcg ttacacccag
      181 ggcccggccg cgcggaatta tactcacctt gtagaccgga tcgtggtcgg gcatgagccg
      241 acccacaatg gcgtgcccgg cttcgtgata ggcggtgttt cgcttctcct tctccgacat
      301 gaccatggat ttgcgctcag cgcccatcat gatcttgtcc ttggccttgt cgaactcttc
      361 catttcgacc agccgcttgc cccctcgagc ggcaaacagc gcggcttcgt tcaccaggtt
      421 cgccaggtcg gccccggaga aaccagggga gccgcgggcg atgatggccg ggtccacatc
      481 gtcagccagg ggtaccttgc gcatgtgaac cttcagaatc tgctcgcggc ccctgatatc
      541 cggaaggctg accaccacct gacgatcgaa acggcccgga cgcagcaacg ccgggtccag
      601 cacgtccggc cggtttgtcg ccgcgatcac gatgatgccg tcgttcggct cgaacccgtc
      661 catctcgacg agcagctggt tgagcgtctg ctcccgctcg tcgtgaccgc cacccagacc
      721 agcaccccgg tgccggccga ccgcatctat ctcgtcgatg aagatgatgc aaggcgcctg
      781 ttttttggcc tgctcgaaca tgtctctgac tcgggaagca cccacgccca cgaacatctc
      841 gacgaagtca gagcctgaaa tcgagaagaa cgggaccttc gcttcaccgg cgatcgcctt
      901 ggcaagcagg gtcttacccg ttcccggctg gccaaccata agtataccgc ggggaatgcg
      961 cccaccaagg cgttggaact tgccgggatc ccgcagaaac tccaccagct cctgcacatc
     1021 ctctttcgct tcgtccacac cggcgacgtc ggcgaacgtg gtcttgatct gatcctctga
     1081 cagcaatcgg gccttgctct tgccgaaagc catgggcccg ccgcggccgc cggcaccgcc
     1141 ctgcatctgg cgcatgaaca gcatgaaaac cgcgatgatg atgaggatgg ggaagctcgc
     1201 gatgagcagc tgcgcccaga tgctttgctg ctcgggcagc ttgcccatga tctgaacgtc
     1261 gtgattgtaa aggtcgtcga gcagcttggg atcgtagagg tcggggcggg tcacctggaa
     1321 ctcttccccg ctgtctcgga caccgcgaat aatcaggccc tcgatttcga ccttgtccac
     1381 ctgatcgttg cgcacctgat cgatgaaggc ggagtaactc agggactctg gctccggctt
     1441 cacgttgaag ttattgaaca ccgtcagcag cacggccgcg atgatcagcc agagcagcaa
     1501 attttttccc atatctgtca agaacttatc ctcaaaactc ggcgcagggg actcgtatcg
     1561 tgcgtccgta accgcgggcg gcaacagcag gcaggcagac gcgatcggcg cccatctgaa
     1621 tagcctacac cactcctatt gagcaaggaa ctgctgccca acaacgtata tctcgcggga
     1681 ctcctgccgg gacgcgtcgg gtttcgctcg gcgaatttta gcaaattttt tgcctatttg
     1741 gcgaatccag gcatccgtgc cttcgcccat gaacatcttg accaccaaaa cgccgccggg
     1801 tttcaaccag cgttccgcgg ccgccatggc gagttccgcc aaatccagag tctcggcctg
     1861 atcccgcgtg cggataccag taatgttggg ggacatgtcc gaaagtacaa ggtccacgcc
     1921 gcgatcaccc aaggctcccg cgatggcttc gtccaccacc gcgtcaccga aaagcccgct
     1981 caccactaca gtatccgccc cagcgtgaac gggtcgtgaa tcgcacacga tcagcctgcc
     2041 cgacggtccc agacgggact cgaggtaggc cgtccagccg ccgggagcgg cgccgagctc
     2101 caggacccac atgccaggag agatcagccg gaatcgtcgg tcgagctgat cgagcttgaa
     2161 gtgcgcgcgg gacacgctcc cctcctgcac cgcacgccgg gcatacggat cgttccgctg
     2221 gcgctcaagc caacggccgc tggagccccc ccgcttcttg gatccgcgag tactcatcgt
     2281 ttcactatg
//
LOCUS       DBNHQR010000187         1615 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1348026, whole genome shotgun sequence.
ACCESSION   DBNHQR010000187 DBNHQR010000000
VERSION     DBNHQR010000187.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1348026"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="AC16VR_04275"
     CDS             complement(<1..197)
                     /locus_tag="AC16VR_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010405.1"
                     /translation="MLADVPPERLLLTFLALTAIIAFRYMLISGLFYWLLWRRKSSGA
                     GAIKLMAGQPAAGAVQKEIGWS"
     gene            260..1432
                     /gene="crtY"
                     /locus_tag="AC16VR_04280"
     CDS             260..1432
                     /gene="crtY"
                     /locus_tag="AC16VR_04280"
                     /EC_number="5.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045436 - lycopene beta cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene beta-cyclase CrtY"
                     /protein_id="HIR6010406.1"
                     /translation="MHYDIIFVGGGLAGSLCAYRLRQLRPELGVLVLEQEARLGGNHT
                     WSFFASDLDEAELGWTEPFVAHQWDGYEVRFPGRRRRLTTGYRSSNSDRLHAVVSAAL
                     GDGVRCGAAVAAMDAGSVRLRDGETLTARCVVDARGPDRAPRLELGFQKFTGLILDLA
                     APHGLEVPIIMDATVDQGGDYRFVYVLPLGPSEVLVEDTRYSVTPGRDALSDESAVRG
                     YAQARGWQVQSVRGREEGVLPITLGGDIEGLWQDAAGMPLIGLRAALFHATTGYSFAD
                     AVRTADAIATSADLSSEALGGLIRRRSIRHWRGQSYLRFLNRMLFLAARPGQGYRVME
                     RFYGLPQGLIERFYADRLTSLDRLRILTGKPPVAILPALRCLSESSVKRFHSGIEF"
     gene            1429..>1615
                     /locus_tag="AC16VR_04285"
     CDS             1429..>1615
                     /locus_tag="AC16VR_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HIR6010407.1"
                     /translation="MNTSQRPTAAVIGSGFGGLALAIRLQSAGFRTTLFEGRDRPGGR
                     AYVYEDEGFTFDAGPTVI"
BASE COUNT          251 a          496 c          542 g          326 t
ORIGIN      
        1 gaccagccga tttccttttg caccgcgccg gcagctggct ggccggccat gagcttgatc
       61 gcccctgcgc ccgatgactt tcggcgccac agcagccagt agaacagacc ggagatcagc
      121 atgtacctga acgcgatgat cgcggtcagc gcgaggaacg tgagcaacag cctttccggg
      181 ggaacgtcgg ccagcatggg ttgggcggag tgaggagctt cttcgctaga atgtcggcag
      241 tttagcgggg actcgccgat tgcactacga catcatattc gtcgggggtg ggctcgccgg
      301 tagcctatgt gcttaccggc ttcgtcagct gcgtccggag ctcggcgtgc tcgtcctgga
      361 gcaggaagcg cgcctcggcg gtaaccacac ctggtcgttc ttcgcttcgg atctcgacga
      421 agccgagctt ggctggacgg aacctttcgt ggcgcatcag tgggacggct acgaggtccg
      481 ctttccggga cgccgccgca ggctcacgac cggataccgg tcgtccaact cggatcggct
      541 ccatgccgtg gtgtctgcgg cgctcggcga tggggttcgc tgcggggcgg cggtggcggc
      601 aatggatgcc ggttcggtca ggctccggga tggagaaacc ctcacggccc ggtgcgttgt
      661 cgatgctcgt ggcccggaca gggcaccgcg cctggaactc gggtttcaga aattcaccgg
      721 gttgattctg gatctcgccg cgccccatgg tctggaggtg cccatcatca tggatgcaac
      781 ggttgatcaa ggaggcgact accggttcgt ttacgtcctt cccctgggtc cgtcggaggt
      841 gctcgtggag gacacccgct acagcgtgac tccgggtcgg gacgcccttt cggacgagag
      901 cgccgtgcga ggatacgcgc aagcccgtgg ctggcaggtg caaagcgtca ggggccggga
      961 ggaaggcgtg cttccgatta cccttggcgg ggacatcgaa gggctttggc aggatgctgc
     1021 cggcatgccc ctgatcggcc tgcgtgccgc tctgttccac gccaccacgg ggtactcgtt
     1081 tgccgatgcc gtccgcacgg cagacgccat cgccacgtcc gcggatctat cctccgaagc
     1141 gcttggcggc ctcattcgac gccggtcgat ccgccactgg cgtgggcagt cttatctgag
     1201 gtttctcaat cgcatgctgt ttctcgctgc gcggccgggc cagggatatc gcgtgatgga
     1261 gcgattctac gggttgccgc aaggtctgat cgagcggttc tacgcagacc gtctcacgag
     1321 tctcgatcgt ttgcggatat tgactggcaa acccccggtt gccattctgc ctgcattgcg
     1381 ctgcctgtcg gagagcagcg tgaagcgatt tcactcgggg atcgagtttt gaacacttcc
     1441 cagcgaccga cagccgccgt catcggatcc ggctttggtg ggcttgcgct cgccatacgc
     1501 ctgcaatcgg ccggcttccg cacgaccctc ttcgagggcc gcgaccgtcc gggtggccgg
     1561 gcctacgtct acgaggacga ggggttcacg tttgacgcgg ggcccacagt catta
//
LOCUS       DBNHQR010000188         8637 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1728197, whole genome shotgun sequence.
ACCESSION   DBNHQR010000188 DBNHQR010000000
VERSION     DBNHQR010000188.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8637)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8637)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8637
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1728197"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="AC16VR_04290"
     CDS             complement(<1..959)
                     /locus_tag="AC16VR_04290"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HIR6010408.1"
                     /translation="MTAASQPGAPERRNYSMFDAEISGAVLNLALLRRLLKWLRPYRV
                     TFGISAVLVLLASTLQVLMPIIISLVVIDHLIRGETDSLAPDLGMVELTEWLADILAL
                     HPLMAACLLYAGLQIAWAAAGHAHRMTLISSVINGLRDLRLDLFRHLETRPSSFYDRV
                     AVGRVMTRVTNDIEALYELLRGLGSLIGEFMPFFVALAIMLAIDVELTLLLLLALPVM
                     GSVTFYFRRATRRLFRMVRMSLSALNQNMQENLAGLQVVQLSERQQYNLDRYTKINEE
                     NRGHELRTTRFETLYGAFNDSMAHLALGVIIWYAGGAAVQEEMT"
     gene            complement(956..2716)
                     /locus_tag="AC16VR_04295"
     CDS             complement(956..2716)
                     /locus_tag="AC16VR_04295"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6010409.1"
                     /translation="MIRSLIRLKSFLAPYRLRLGAGVGAFGIARFFEAMVPIFVAIGI
                     DRMSAGNADMTTPLLGIAGAVIGRYVVVTYARYSVRCVGQHVAFDLRQALYESLQQQG
                     VRFFTRYTIGDMMTRAVADIGLIQRLIAMGTILMVILVYATLVGFGFMLYLSPSLTLL
                     LLPPLPLVFFYASWSARQMGVASRDVQNRLSDLGAHAQENLSGIRTIQAMVQEENEIR
                     RFGRTNQQYANAFYRQSQINSLMAAWMPSLAAICSVTILGYGGYLVLDGAMTVGELVA
                     FFMYVNMVVQPFRVAGFIVNLFQRAAVASDRLFEVITLSPEIADDPSGTTPATIRGDV
                     EIRHLTFAYDEGIRPALDDVSLTVARGESVTIMGRVGSGKTTLLKQLVRLLDTPPGAV
                     LIDGHDVRDYPLAQLRSQVAMVPQDAFLFGEPLGDNLTYDDPDRAVEKIWEAATSADL
                     KETIEEFPEQLDTIVGERGVTLSGGQKQRATLARGLIRGAPVLILDDCFSSVDTETEE
                     HILRELRRLRRRQTTILVSHRVSTARHSDRIVVMDAGRIVESGTHEELLARAGQYAEL
                     ERIQREGAVDTDYDLIGATT"
     gene            complement(2741..4132)
                     /gene="asnS"
                     /locus_tag="AC16VR_04300"
     CDS             complement(2741..4132)
                     /gene="asnS"
                     /locus_tag="AC16VR_04300"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF003037.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HIR6010410.1"
                     /translation="MTDTIKDILAHRLPVGAEVTIEGWVRSRRTSKGGFSFVHVSDGS
                     CFDTIQAVADSQLANYEHDVEHLGTGCSVRIRGTVVESQGRGQDREIQATEVVVVGWV
                     DDPETYPIAKKRHTFEYLREVAHLRPRTNTFGAIARVRNAIAQAVHGYFNDNGFVWVN
                     TPIITASDCEGAGQLFRVSTLDIANRALTRGDSPFDDDFFGRETFLTVSGQLNLESYC
                     LALSKVYTFGPTFRAENSNTSRHLAEFWMIEPEIAFADLNANAELAEDFLKAVCRRVL
                     DACPDDMSFFADRVDEQAISRLEHVIATPFERIDYTDAVSILEKAGQNFEYPVQWGLD
                     LQSEHERYLTEVHVGGPVVVVNYPKTIKAFYMRLNDDDATVAAMDVLVPGVGEIIGGS
                     QREERLDVLDARMAEQDIGEDLWWYRDLRRYGTAPHAGFGLGLERLVLYMTGMDNIRD
                     AIPFPRVPHNASF"
     gene            4230..5006
                     /locus_tag="AC16VR_04305"
     CDS             4230..5006
                     /locus_tag="AC16VR_04305"
                     /inference="COORDINATES: protein motif:HMM:NF022665.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3014 domain-containing protein"
                     /protein_id="HIR6010411.1"
                     /translation="MRNLIIVIALVLVLLGGLWLASDRSAGDREVPLPGAEPVPVPGP
                     RPAEPARRPQPMPVAEPAIVLPDLDASDPFLRERLAGVELPQNWIDRDDLVRRLAVVI
                     ENAARGEYPRRQLGFLAPSGRFRVVERQGRLFVDPDNYRRFDGYLDVLEGLDPRTAAE
                     LLSLIEPLLAVAMGELGSDGSVSDQIDAAVAEVMAVPDVVGEVELAQPKVLYEYADPA
                     LEALSPLQKQVLRMGPENAARLKRYVAEMAVALQPDPSDG"
     gene            complement(5032..6240)
                     /locus_tag="AC16VR_04310"
     CDS             complement(5032..6240)
                     /locus_tag="AC16VR_04310"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6010412.1"
                     /translation="MTSRTARILHSIDEIPAEDWNRCAMPGGEIPHDPFLRHEFLAAL
                     EHSGSAAARTGWQPFHLVLEEADRILGVVPMYVKNHSQGEYVFDYAWADAWHRAGGDY
                     YPKLQVSVPFTPATGRRVLAAGQDEVSRPEIERALLGACAQVAAEIKVSSLHVTFLPE
                     AQWQIAGELGFLRRMDQQFHWHNAGYGSFEEFLGDLSSKKRKNLRRERRDALATGIRI
                     EWLTGSDIQESHWDAFYRFYMDTGSRKWGSPYLTRQFFSLVTETMAEDVLLIMCSRDG
                     RYIAGALNFIGGEALFGRNWGCIEHHPFLHFEACYYQAIDFAIERGLKRVEAGAQGPH
                     KVARGYLPCPTYSAHWIADAGFRNAVARYLDEEREHVREDIEYIEERSPFKSTVSLDK
                     FHNTRTLDIG"
     gene            complement(6307..6768)
                     /locus_tag="AC16VR_04315"
     CDS             complement(6307..6768)
                     /locus_tag="AC16VR_04315"
                     /inference="COORDINATES: protein motif:HMM:NF015325.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HIR6010413.1"
                     /translation="MKHLLIVYHTQTGRTGQLAAAVHRGATNELVSDVDVRLLTAQNA
                     GADDLLWADGLLLGTPENFGYMSGGMKDFLDRTFYPVEGKILSLPYAIFISAGNDGSG
                     ALRAIRRIANGYPFKEVQEPVIARGEITSDHIDRCEELGLTLAAGLEAGIF"
     gene            6837..8042
                     /locus_tag="AC16VR_04320"
     CDS             6837..8042
                     /locus_tag="AC16VR_04320"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF005600.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HIR6010414.1"
                     /translation="MTQLDDITQARQRICGQIELTACSRSATLSAMTGADVHLKFENL
                     QFTASFKERGALNRLLQLTGEQQRFGVVAMSAGNHAQALAYHGKRLGVPTCIVMPRFT
                     PNAKVEQTRVHGAEVLLHGAEFDETLAFTEALAEERGLTLIHPFDDPAVIAGQGTLGL
                     ELVEQVPGLEAVLVPVGGGGLIAGVATAVKSVKPEVELLGVQVERFASVYAAFHGREV
                     PPGPPATVAEGIAVRAPGKNTLPLIRRHVDDVVLVSEEQVEQSIFKLLEIEKTVVEGA
                     GATSLAALLAEPGRFAGRRVACVLSGGNIDMMMLSSVVQRGLVRSRRLVRLSVEIPDV
                     PGALSELTRVLGALDSNIVDIAHQRAFGGSSVRATRVDLVLQMRGEEQVDEVVQELQA
                     RGYECARGG"
     gene            8119..>8637
                     /locus_tag="AC16VR_04325"
     CDS             8119..>8637
                     /locus_tag="AC16VR_04325"
                     /inference="COORDINATES: protein motif:HMM:NF014664.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR6010415.1"
                     /translation="MRVNEPRIPALEQSEWSEAAQEIMGPMVAQDRVFNIFRTLSNHP
                     DLMRRWLVFANHILGKSTLPVRERELVILRIGYLCQSGYEWGQHVRIAQEAGMTAQEI
                     RSAKTGPDTPGLSDLDRLLLKATDELHADAHVSDETWKALTGHLNTQQLMDLVFTVGQ
                     YNMVSMVLNSFGV"
BASE COUNT         1473 a         2654 c         2958 g         1552 t
ORIGIN      
        1 gtcatttctt cctgcactgc agcgccgccg gcgtaccaga tgatcacccc gagggccagg
       61 tgcgccatgc tgtcgttgaa ggcgccgtag agggtctcga atcgcgtcgt tctgagctcg
      121 tggccgcggt tttcttcgtt gatcttcgtg taacggtcca ggttgtattg ctgtcgctcg
      181 gagagctgca ccacctgaag gcccgccagg ttttcctgca tgttctgatt cagagccgac
      241 agcgacatgc gcaccatccg gaacaggcgg cgcgtcgccc ggcggaaata gaaggtcacg
      301 ctgcccatga cgggcaacgc gagaagcaga agcagggtta gctcgacgtc gatggccagc
      361 atgatggcga gcgccacgaa gaagggcatg aactcgccga tgagcgagcc gagaccgcga
      421 agcagctcgt agagcgcttc gatgtcgttg gtcacccgcg tcatgacccg tcctacggcg
      481 actcggtcgt agaacgagga cggtctggtc tcgaggtgtc gaaacagatc cagccgcagg
      541 tccctcagac cgttgatgac cgacgagatc agggtcatgc ggtgagcgtg accggcagcc
      601 gcccaggcaa tctgcagccc cgcatacagg agacaggccg ccatgagagg gtgaagtgcc
      661 aggatgtccg ccagccactc ggtcagctcc accatgccga ggtccggtgc cagggaatcc
      721 gtctcgcccc ggatcaggtg gtcgatcacc accagcgaga tgatgatggg catgagaacc
      781 tgcagcgtgg acgccagcag caccagcacg gcgctgattc cgaaggtcac ccggtagggc
      841 cgcaaccatt tcaggaggcg tcgcaggagc gcaaggttca gaaccgcgcc ggagatctcg
      901 gcgtcgaaca tggagtagtt gcgccgctcg ggagcgccag gctggctggc cgccgtcacg
      961 tcgtcgcccc gatcaggtcg tagtcggtat caacagcgcc ctcgcgctga atccgttcga
     1021 gctcggcgta ctgtcctgcc ctggccagaa gctcttcgtg ggtaccggac tcgacgatgc
     1081 gcccggcgtc catgaccacg atgcggtcgg agtggcgggc ggtcgaaacg cggtgggaca
     1141 ccaaaatggt ggtctggcgg cgccgcagcc ggcgcagctc ccggagaatg tgctcttccg
     1201 tctcggtgtc gacgctggaa aagcagtcgt cgaggatcag cacgggcgct ccgcgaatga
     1261 ggccgcgggc cagggtggct cgctgcttct ggcctccgga gagcgtcacg ccgcgctcgc
     1321 cgacgatggt gtcgagctgc tccgggaact cctcgatggt ttccttgaga tctgcggacg
     1381 ttgccgcctc ccagatcttc tccaccgccc ggtccgggtc gtcgtaggtc aggttgtcgc
     1441 ccaggggctc gccgaacaga aaagcatcct ggggcaccat ggcaacctgg gacctcagct
     1501 gggcgagtgg gtagtcgcgg acgtcgtggc cgtcgatgag aaccgcgcct ggcggcgtgt
     1561 ccagcagccg cacgagctgc ttgagcagcg tcgtcttgcc cgatccgacc ctgcccatga
     1621 tggtcacgga ctcgccccgg gcgacggtca gcgagacgtc atcgagagcc ggacgaatgc
     1681 cctcgtcgta agcgaaggtc aggtggcgta tctcgacgtc gccccggatc gtcgccggcg
     1741 tcgtcccgct tggatcgtcg gcgatctctg gggacagggt gatgacctcg aacagccggt
     1801 cgctggccac ggccgcccgc tggaagaggt tgacgatgaa gccggccacc cgaaacggct
     1861 ggacgaccat gttcacgtac atgaagaagg caacgagctc gcccaccgtc atggcgccgt
     1921 ccaggaccag gtagccgcca taccccagga tggtgacgga gcagatggcg gccagcgaag
     1981 gcatccaggc cgccatgagc gagttgatct gagactggcg gtagaacgcg ttggcgtact
     2041 gctggttggt cctgccgaac cggcggatct cattctcttc ctggaccatg gcctggatgg
     2101 tgcgaatccc ggagaggttt tcctgggcgt gggcgcccag gtcggacaat cggttctgaa
     2161 cgtccctcga ggccacgccc atctggcgcg cggaccagct tgcgtagaag aacaccaggg
     2221 gcaggggtgg cagcagcagc agcgtgagtg acggggacag atagagcatg aagccgaagc
     2281 ccaccagcgt cgcgtagacg aggatgacca tcaggatcgt gcccatggcg atgagtctct
     2341 ggatcaggcc gatgtcggct accgcccggg tcatcatgtc gccgatggtg tagcgcgtga
     2401 agaaacgaac gccctgttgc tgcagggatt cgtacagagc ctgccgcaga tcgaacgcca
     2461 cgtgctggcc gacgcagcgc acggagtagc gggcgtaggt gaccacgacg tagcgcccga
     2521 tcaccgctcc ggcaattccg agcagtgggg tcgtcatgtc cgcgttgccg gcggacatcc
     2581 gatcgatgcc gatggccacg aagataggca ccatggcttc gaagaatctt gcgattccga
     2641 acgcgccgac gccggccccc agtcgcagcc gatacggggc gaggaacgat ttcagacgga
     2701 tcagagagcg aatcatcggt ggggcgcagc cgctgccgcg tcagaaactg gcgttgtgcg
     2761 gcactctggg gaacgggatg gcgtcacgga tgttgtccat ccccgtcatg taaagcacca
     2821 ggcgttccag accgagcccg aaccccgcgt ggggtgcggt gccgtaccgg cgaaggtccc
     2881 ggtaccacca gagatcctcg ccgatgtcct gttccgccat gcgggcgtcg agcacgtcca
     2941 ggcgctcttc ccgctggctg ccgccgatga tctcgccgac gccggggacg agcacgtcca
     3001 tggcggccac cgtcgcgtcg tcgtcgttca ggcgcatgta gaacgctttg atggtcttgg
     3061 ggtagttgac caccaccacc ggcccgccca cgtgcacctc ggtgagatag cgctcgtgct
     3121 cggattgcag gtcgagtccc cactgcaccg ggtactcgaa gttctgaccc gccttctcga
     3181 ggatgctgac ggcgtccgtg tagtcgatcc gttcgaacgg tgtggcgatg acgtgttcca
     3241 ggcggctgat ggcctgctcg tcgacccggt cggcgaagaa actcatgtcg tcgggacagg
     3301 cgtccagcac cctgcggcag acggctttca ggaagtcctc ggcaagctcc gcgttggcgt
     3361 tcaggtcggc gaaggcgatt tccggttcga tcatccagaa ctcggccaga tgtcggctgg
     3421 tattcgagtt ctccgcgcgg aaggtcgggc cgaacgtgta caccttgctc agagcaaggc
     3481 agtagctttc caggttcagt tgccccgaga cggtcagaaa ggtctcgcga ccgaagaagt
     3541 cgtcgtcgaa ggggctgtcc ccgcgggtca gtgcccggtt ggcgatgtcc agcgtggaga
     3601 cgcgaaacag ctgcccggcg ccctcgcaat cgctggccgt gatgatcggc gtgttgaccc
     3661 agacgaaacc gttgtcgttg aaatagccgt gcaccgcctg ggcgatggcg ttacgcacgc
     3721 gagcgatggc gccgaaggtg ttcgtgcggg gacgcagatg ggcgacttct ctgaggtatt
     3781 cgaaggtgtg gcgttttttt gcgatcgggt aagtttcagg gtcgtccacc catcccacca
     3841 ccacgacctc ggtggcctga atctcgcggt cctggccgcg gccttgagat tccaccacgg
     3901 ttcccctgat ccgcacggag cagcctgtgc cgagatgctc gacatcgtgc tcgtagttgg
     3961 cgagctgcga gtcggccacc gcctgaatgg tgtcgaagca ggagccgtcg ctgacgtgca
     4021 cgaaggagaa gccgcctttc gacgttcgcc tcgatctgac ccaaccttcg atggtgacct
     4081 ctgctccgac gggaaggcgg tgggcgagga tgtctttgat ggtgtcggtc atcggcgcgg
     4141 ctgctcaggc aggaatgagg gattgtggaa tgataatgga attggcccgg ccttggatca
     4201 ttccccgcgg gtcgtggtca taatgagaca tgcgcaacct catcatcgtc atcgccctcg
     4261 tgctggttct gctcggcggc ctctggctgg cttcggatcg cagtgccggt gatcgggaag
     4321 tcccgctccc gggggccgag ccggtgccgg ttcccggacc gcggcccgct gaacccgcgc
     4381 ggcgaccgca gccgatgccc gtcgctgagc cggctatcgt gctgcccgac ctcgacgcga
     4441 gcgatccgtt cctgcgggaa cggctggctg gggtcgagtt gccccagaac tggatcgatc
     4501 gtgatgatct cgtccgccgc ctggcggtgg tgatcgaaaa cgcggctcgc ggagagtatc
     4561 caaggcgtca gctgggtttt cttgcgccgt ccggccgttt ccgcgtggtg gaaagacagg
     4621 gcaggctttt cgtggacccc gacaactacc gacgcttcga cgggtatctc gacgtgctcg
     4681 aaggcctgga cccccggacg gccgccgagc tgctgtctct catcgagcct ctgctggccg
     4741 tcgccatggg cgagctcggc agcgacgggt cggtttccga tcagatcgat gctgccgttg
     4801 cggaggtgat ggcggtgccc gacgttgtcg gcgaggtgga gctggctcag ccgaaggtgc
     4861 tctacgagta cgcggatccg gcgctggaag cgctgtcgcc gctgcagaag caggtgcttc
     4921 gaatgggtcc ggaaaatgcg gcgcggctca agcgctacgt tgccgagatg gccgtcgcgt
     4981 tgcagcctga cccctccgac ggctaggcgg gcgcccttcg gcgcaagccg ttcagccgat
     5041 gtccagggtg cgggtattgt ggaacttgtc caggctgacg gtggacttga aggggctgcg
     5101 ttcctcgatg tactcgatgt cctcgcggac gtgctcgcgc tcctcgtcca gatagcgggc
     5161 aacggcatta cgaaagcccg cgtcggctat ccagtgggcg ctgtaggtag ggcagggtag
     5221 atacccccgg gcgaccttgt gcgggccctg ggcgccggct tccacccttt tcagtccccg
     5281 ctcgatagcg aagtcgatgg cctgatagta gcaggcttcg aagtgcagaa aggggtggtg
     5341 ctcgatgcaa ccccagttgc gaccgaagag ggcttccccg ccgatgaagt tgagcgcgcc
     5401 cgcgatgtag cgcccgtccc ggctgcacat gatcagcagc acgtcctcgg ccatggtctc
     5461 ggtgacgagg ctgaaaaact gccgagtgag gtatggggac ccccattttc gggatcccgt
     5521 gtccatgtag aagcggtaga aggcgtccca gtgcgattcc tggatgtcgc tccccgtgag
     5581 ccattcgatc ctgatgccgg tggccagggc gtcccgccgc tcgcggcgga ggttcttgcg
     5641 cttcttcgag gagagatcgc cgagaaactc ctcgaagctc ccgtagcctg cgttgtgcca
     5701 gtggaactgc tgatccatgc gccgaagaaa gccgagctcg ccggcgatct gccactgggc
     5761 ctccggcagg aaggtgacgt gcagggacga gactttgatc tctgccgcta cctgggcaca
     5821 cgcccccagc agcgcccgct cgatctccgg ccgcgagacc tcatcttggc cggcggcaag
     5881 cacgcgccga ccggttgccg gcgtgaacgg aacgctgacc tgcagcttgg ggtagtagtc
     5941 gccgccggcc cgatgccagg cgtcagccca ggcgtagtcg aacacgtact cgccctggga
     6001 gtggttcttc acgtacatgg gcaccacgcc cagaatacgg tcggcttctt caaggaccag
     6061 gtgaaagggt tgccagcccg tcctggccgc ggcgctgccg ctgtgctcga gggcagccag
     6121 aaactcgtgg cgcaggaacg ggtcgtgggg aatctcgccg cctggcatcg cgcaccggtt
     6181 ccagtcttcg gccggtatct cgtcgatgct gtgcagaatt ctcgcggtgc gggaagtcaa
     6241 tcggttctcc ttcgttccga gtttaaccgc tcgccgcaag gtgcgctgcc ctggcaggcg
     6301 aacgcttcag aaaataccgg cctcgaggcc ggcggccagg gtcaggccga gctcttcgca
     6361 gcggtcgatg tggtcgctgg tgatctctcc ccgggcgatg acgggctcct ggacttcctt
     6421 gaacggatat ccgttggcga tgcgtcggat ggcgcgcagc gccccgctgc cgtcgtttcc
     6481 ggcgctgatg aagatcgcgt agggcaggga aagaatcttc ccctccacgg ggtagaacgt
     6541 ccggtcgaga aagtccttca tgccgccgga catgtagccg aagttctcgg gcgtgccgag
     6601 caggaggccg tcggcccaga ggagatcgtc tgcccccgcg ttctgcgccg tcagcaggcg
     6661 gacgtccacg tcgctcacga gctcgttggt ggcacctctg tggacggccg ccgccagctg
     6721 gccggtgcgg ccggtctgcg tgtggtagac gatgagaaga tgcttcatct gctggcggga
     6781 ggtgggcgag gctggtgggt cgtcgagtat actgacgcct tttcagaagt tcgatcgtga
     6841 cccagctcga cgacattact caggcccggc agcgcatctg cggccagatt gagctgacgg
     6901 cctgcagccg ctcagcgacg ctgtcggcaa tgacgggcgc ggacgtccat ctcaagttcg
     6961 agaacctgca gttcacggcg tcgttcaagg agcgcggagc gctcaaccgg ttgctccagc
     7021 tgacgggcga gcagcagcgc ttcggcgtgg tggccatgtc ggcgggcaat cacgcccagg
     7081 ccctggccta tcacggcaag cggctgggcg tacccacctg catagtcatg ccccggttca
     7141 ctccgaacgc gaaggtcgag cagacgcgcg tccacggcgc ggaagtgctg ctgcacggcg
     7201 ccgagttcga cgagacgctg gcgttcaccg aagccctggc cgaggagcgc ggcctcacac
     7261 tcattcaccc cttcgacgat ccggcggtca ttgctggcca gggcaccctg gggctggagc
     7321 tcgtggagca ggtcccggga ctcgaggcgg tgctggttcc ggtggggggc gggggcctga
     7381 tcgccggggt ggcgacggcg gtgaaatcgg tgaagccgga ggtggagttg ctcggcgtgc
     7441 aggtggaacg cttcgcttcg gtctatgcag cctttcacgg ccgcgaagtg ccgccggggc
     7501 cacccgcgac ggttgctgaa ggcatcgcgg tgcgcgcccc gggaaagaac accctgccgc
     7561 tgatcaggcg gcacgtagac gatgtcgtcc tcgtctccga ggagcaggtg gagcagagca
     7621 tattcaagct gctggagatc gagaaaaccg tggtcgaggg ggccggcgcc accagcctgg
     7681 ccgctctgct ggctgagccc gggcgttttg ccggccgccg ggtggcctgc gtgctcagcg
     7741 gcggcaacat cgacatgatg atgctgtcgt cggtcgttca gcgcggtctg gttcgcagcc
     7801 ggcggctcgt tcggctgagc gtggagattc ccgacgtgcc gggcgcgctc agcgagctca
     7861 cgcgggtgct gggcgccctg gacagcaaca tcgtcgacat tgcccatcag cgagccttcg
     7921 gcggctcatc ggtgcgggcc acgcgggtag acctggtgct gcagatgcgc ggtgaggagc
     7981 aggtggacga ggtcgtccag gagctccagg cgcggggcta cgaatgcgcc cgcggcgggt
     8041 gaatcccggc ggcgcgccat cgtctgtcgg gttatattga cggggcaatc ggaaccagag
     8101 aactttcggg gaggcgtcat gcgcgtaaac gaacccagga tacctgcgct cgagcaatcc
     8161 gagtggagcg aggctgctca ggagatcatg gggccgatgg ttgcccagga tcgggtcttc
     8221 aacatcttca ggacgctttc caatcacccg gacctgatgc gccgctggct ggtgttcgcg
     8281 aatcacatcc tgggcaagtc caccttgccc gtccgtgagc gcgagctggt gatcctcagg
     8341 atcggctatc tgtgccagtc ggggtacgag tgggggcagc acgtccggat cgcgcaggaa
     8401 gcggggatga cggcgcaaga gatccgctct gcgaaaacgg gcccggacac tcccggcctt
     8461 tccgatctgg accgcctgct gttgaaggcc accgatgagc tccacgcgga cgctcacgtg
     8521 agcgacgaga cctggaaggc gctcaccggg catctgaaca cccagcagct catggatctg
     8581 gtcttcaccg tggggcagta caacatggtt tccatggttc tcaacagctt cggggta
//
LOCUS       DBNHQR010000189         2424 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1728226, whole genome shotgun sequence.
ACCESSION   DBNHQR010000189 DBNHQR010000000
VERSION     DBNHQR010000189.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1728226"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="AC16VR_04330"
     CDS             <1..381
                     /locus_tag="AC16VR_04330"
                     /inference="COORDINATES: protein motif:HMM:NF052838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6010416.1"
                     /translation="QGTIVRTEAQIDTASRMVHVVARISNVEQPVPLNVGLFVNAEIE
                     GLLADNIVVLPRTALRDGDRVLVVDGGNNLRYRDIEPLRLYKDEVLIKAGLAAGERVC
                     VSPLQTAVDGMPVQPVTENAPTAG"
     gene            386..>2424
                     /locus_tag="AC16VR_04335"
     CDS             386..>2424
                     /locus_tag="AC16VR_04335"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6010417.1"
                     /translation="MYTAISWFTRNPVAANLLMFVLLIGGCLALFNVHQEEFPSMDVR
                     VVNVTVPYLGAAPEEVEEGVCIRVEEALEGTEGIEKLLSAAREGSCSVQAMLYEDANQ
                     TEALNEIKSQVDGINTFPLETEKPIVSKLTITSDVLQIAVYGDTDERTLKELGKELRD
                     ELSMIDGISKIALNYVRPYEISIEISEDTLRRYGITLEQVARVIRNTSMDMPGGTIKT
                     KGGEILLRAKGQAYWGEEFEDIVVVTRNDGTKVMLSEIAEIRDEFEEGDMRARFNGYP
                     AAVVKVWQVGKEDALEMAADIKAYLTRYEKTLPAGLFVKVWNDESEQLKARLDTLNGM
                     ALSGLGLVVIILAMFLKFRLAMWVAAGIPIALLGTLAVFPYADISMSAMTVMAFILVL
                     GILVDDAIVVGERVYGHEQMGKPPVQAAIDGTWEVSIPVIFGVLTTMAAFLPLLLSTG
                     RMAGFFSVVGWVVIIALVFSIVESQLILPSHLAHRNNDEASNSFSRRWNRIQGRLARW
                     LENIANYRYEPFLRKAIEYRYITASVGLGLLIMSVALIASGRVVFAFFPGIEGDRIYA
                     TLEMPEGISVEATGEAVDIIEAAGERLGKALDAELNQTATIRNVFTSIGMPADRQNGP
                     SRGGSAGRSHFAEVVLELAPGEERNYLSAKEIAHRWRKLVGNIPDAVKLSFTADA"
BASE COUNT          452 a          767 c          784 g          421 t
ORIGIN      
        1 cagggaacca tcgttcgaac cgaagcccag atcgacacgg cgagccgcat ggttcacgtc
       61 gtcgcccgga tttccaacgt ggagcagccg gtgccgctca acgtgggctt gttcgtcaac
      121 gcggagatcg aggggctgct ggccgataac atcgtcgttt tgccgagaac ggcgctgcgc
      181 gacggcgatc gagtgctggt ggtggatggc ggaaacaacc tgcgctatcg ggatatcgag
      241 cccctgcgcc tgtacaagga cgaggtgctg atcaaggcgg ggttggccgc cggcgagcgc
      301 gtctgcgtat ctcccctgca gaccgccgtc gatggcatgc cggtccaacc cgtcacagag
      361 aacgccccga cagccggctg agcccatgta caccgcgatt tcctggttca ctaggaaccc
      421 cgttgccgcg aacctgctta tgttcgtcct gctgatcggc gggtgcctgg cgcttttcaa
      481 cgtccatcag gaagagttcc ccagcatgga cgttcgcgtc gtgaacgtca ccgtgcccta
      541 cctcggcgcg gcgcccgagg aggtggagga aggcgtgtgc atccgggtcg aggaggccct
      601 tgaaggcacc gagggcatcg agaagttgct gtccgccgcg cgcgagggct cctgcagcgt
      661 gcaggccatg ctctacgagg acgccaacca gacggaagcg ctgaacgaga tcaaaagcca
      721 ggtcgacggc atcaacacgt ttccgctcga gacggaaaag cccatcgtct cgaagctgac
      781 gatcaccagc gacgtcctgc agattgccgt ctacggcgat acggacgagc gcaccctcaa
      841 agagctcggc aaggagctgc gcgacgagct atccatgatc gacggcatct ccaagatcgc
      901 gctcaactac gtgcggccct acgagatctc catcgagata tcggaggata cgctgcgccg
      961 gtacggcatc accctggagc aggtggcccg ggtgatccgc aacacgtcca tggacatgcc
     1021 cggcggcacc atcaagacca agggcggaga gattctcctg cgggccaagg gccaggccta
     1081 ctggggcgag gagttcgaag acatcgtggt cgtcacgcgc aacgacggca ccaaggtgat
     1141 gctgagcgag atcgccgaga tccgcgacga attcgaagag ggcgacatgc gcgcccgctt
     1201 caacggatac cccgccgccg tggtcaaggt ctggcaggtt ggcaaggaag acgcgctcga
     1261 gatggcggcc gacatcaaag cgtacttgac ccgctacgag aagacgctgc cggccgggct
     1321 gttcgtgaag gtgtggaacg acgagtccga gcagctgaag gcccgcctgg acacgctgaa
     1381 cggcatggcg ctttccgggc tcgggctcgt ggtcatcatc ctcgccatgt tcctgaaatt
     1441 caggctggcc atgtgggtgg cggccggcat ccccatcgcg ctgctcggca cgcttgccgt
     1501 gtttccctat gcggacatct ccatgagcgc catgaccgtc atggcattca tcctggttct
     1561 gggcatcctg gtggacgacg ccatcgtcgt cggcgagcga gtatacggtc acgagcagat
     1621 gggcaagccg ccggtgcagg cggccatcga cggcacctgg gaggtctcga ttcccgtgat
     1681 cttcggggtg ctgaccacga tggccgcctt cctgcccctg ctgctcagca ccggccggat
     1741 ggccggcttc ttcagcgtcg tgggctgggt cgtcatcatc gccctggtgt tcagcatcgt
     1801 cgagtctcag ctgatccttc cgtctcacct cgcccaccgc aataacgacg aggcgtccaa
     1861 ttctttctcc cggcgctgga accgcatcca gggccgcctg gcgcgctggc tggagaacat
     1921 cgccaactac cgctacgagc ccttcctgcg caaggcaatc gagtatcgct acatcacggc
     1981 gtccgtgggg ctcggcctgc tgatcatgtc agtggcgttg attgccagcg gccgagtggt
     2041 gtttgccttt ttcccgggca tcgagggaga ccgcatctac gccactctgg agatgccgga
     2101 aggcatttcg gtggaggcga ccggcgaggc cgtcgacatc atcgaggccg ccggtgagcg
     2161 gctcggcaag gcgctcgacg ccgagctgaa ccagaccgca acgattcgca acgtgttcac
     2221 ctccatcggc atgcccgcgg accggcagaa cggtccatca cgtggcggaa gcgccggccg
     2281 gtcgcacttc gccgaggtcg tgctggagct ggcaccgggg gaggaacgca actacctgag
     2341 cgccaaggaa atcgcccatc ggtggcggaa gctggtgggc aacattcccg acgcggtgaa
     2401 actctccttc acggccgatg cctt
//
LOCUS       DBNHQR010000190         1055 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1602157, whole genome shotgun sequence.
ACCESSION   DBNHQR010000190 DBNHQR010000000
VERSION     DBNHQR010000190.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1602157"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            297..>1055
                     /locus_tag="AC16VR_04340"
     CDS             297..>1055
                     /locus_tag="AC16VR_04340"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6010418.1"
                     /translation="MAEKVETVRTTDPAYHRLLDTETRPVPESYRRDSPIEPGPTFVP
                     VERYISREFFDLEVEKIWKRVWQMACHEDDLPDVGDYVPYDIAGMSFLVVRTGEDEFK
                     AYYNACLHRGRKLRENRGKAATDLRCPFHGWTWNLDGSLKQIPCQWDYPGLKMSEQSL
                     PEVKVGRWGRYIFINPDPDCGPLEDFLGDLSSHFELLPYEKRYKEAHVARIIRCNWKV
                     ASEAFMESYHVIATHPQIILGGVHDTDTKYDVFGN"
BASE COUNT          195 a          329 c          333 g          198 t
ORIGIN      
        1 agctttcagc cgtgagcgcg gcgggcgccc cgtcctgctc cttcaagatg atgtcgcggg
       61 tgctcggtcc gggacagcgc gcgtctcccg gagccaacgt cgggtcggct ggtttggtga
      121 ggtcgtagtc catcttggta ttccccgggt aggtgctgca ctgatgatac atggttcggc
      181 cagcgagcca accggagcgc ggtgacgtca tcgggttacc tggcgggctc cgcgcgagcc
      241 gtattccttg tgaccagatc ggggtagtat tccggcagtt caggggagaa cgcgtcatgg
      301 cagaaaaagt tgaaaccgtc cgcaccacgg accccgccta tcaccggctg ctggataccg
      361 agacccgtcc ggttccggag tcctaccgcc gcgattctcc catagagccc ggacccacct
      421 tcgtgccggt ggagcgctat atctccaggg agtttttcga cctcgaggta gagaagatct
      481 ggaagcgcgt ctggcagatg gcctgccatg aagacgacct gcccgacgtt ggcgactacg
      541 tgccctacga catcgccggc atgtcgttcc tggtggtccg gaccggggag gacgagttca
      601 aggcctacta caacgcctgc ctgcaccggg gccgcaagct gcgtgaaaat cggggcaagg
      661 ccgccaccga tctgcgttgc ccgtttcacg gctggacctg gaacctggat ggttcgctca
      721 aacagatccc gtgccagtgg gactatcccg gcctgaaaat gtcggagcag agcctgcccg
      781 aggtcaaggt gggtcgctgg ggccgctaca tcttcatcaa ccccgatccg gactgcgggc
      841 cgctggaaga ctttctgggc gatctctcga gccacttcga gctgctgccc tacgagaagc
      901 gctacaaaga ggcccacgta gccaggatca tccgctgcaa ctggaaggtg gcttccgagg
      961 cgttcatgga gtcttaccac gtgatcgcca cccacccgca gatcatcctg ggcggggtcc
     1021 acgacacgga caccaagtac gacgtgttcg gcaac
//
LOCUS       DBNHQR010000191         5429 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1095393, whole genome shotgun sequence.
ACCESSION   DBNHQR010000191 DBNHQR010000000
VERSION     DBNHQR010000191.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5429)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5429)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5429
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1095393"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1522)
                     /locus_tag="AC16VR_04345"
     CDS             complement(<1..1522)
                     /locus_tag="AC16VR_04345"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding response regulator"
                     /protein_id="HIR6010419.1"
                     /translation="MDFKESHVRDLLDAAPDATVVADSAGVIVFANAQVGEVLGYAPE
                     ALIGLNVDQLLPERLRAAHGAHRADFMSAPRVRPMGAGLDLYGRRRDGSEFPIEISLS
                     PVSTDQGMLVISAIRDVTERKAFEVELSAAKDTAERATAIKSRFLAAASHDLRQPLQS
                     IAIYLDVLNRVVRQDQDKALEVSAKIDKSLDVMRELLDALLDVSKLDSGSITPEVRDF
                     RLQSLFDELVADCAPEAEGKGLTLSIEPTEAVVRTDPALLQRILANFVGNAVRYTEEG
                     SVAVSAAESGDHLRVEVRDTGIGIPGEAIDTIFEEYFQLDNPVRDRRKGLGLGLSIVK
                     QVASLLDCPLEVASTVGEGSCFSILVPLGLLDEEETVPGQPQTGQLAPGEPVILFVDD
                     DPAIVDATTMMLELAGISVRSALDGNRAIAMLEEGLSPDMVVSDFRLPGANGIEVIRR
                     ARELRGSYLPTVLMTGDTSGKVIDEAAISNCTVLRKPVDTNHLISLIERGLDAGRGP"
     gene            1707..2774
                     /locus_tag="AC16VR_04350"
     CDS             1707..2774
                     /locus_tag="AC16VR_04350"
                     /inference="COORDINATES: protein motif:HMM:NF024874.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6010420.1"
                     /translation="MLKLKCSVRKAVLIGALAGASPAMAGAPEHHTENGRFTNPYVEQ
                     AQGGVFAFLKRRLLGQDTWSSYDAHRDGAVPTAAPRLVAEGEQTNNARVTWVGHATVL
                     IQHAGVNVLTDPILSRIASPVAFAGPGRKSAPGVDAQGLPPIDVVVISHDHYDHLDEP
                     TIRFLGDRPRYFVPLGLKQWLVDAGIDQSRITELDWWEKQTVEPGGSALTVTATPTQH
                     VSGRTLTDRNKRLWAGWAMEWDDFTVWFGGDTGYNDVQFKAIGDRFDGFDLGIVPIGS
                     YAPRAYMHSYHADPEQAVRIHQDIRARRSMAVHWGTFHLTAEPLLEPPELLRAAVLEA
                     GLPSEEVTAYAVGESRAYFPL"
     gene            2845..2921
                     /locus_tag="AC16VR_04355"
     tRNA            2845..2921
                     /locus_tag="AC16VR_04355"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2879..2881,aa:Val,seq:cac)
     gene            complement(2978..3820)
                     /locus_tag="AC16VR_04360"
     CDS             complement(2978..3820)
                     /locus_tag="AC16VR_04360"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6010421.1"
                     /translation="MRELDGKVAVVTGAASGIGRATATRFAGEGMKVVMADIEEAALE
                     RAVAELSGAGHEVLGVPTDVSDWEAVSLLADKTIDAFGSVNVVHNNAGVVVSGPIADL
                     TLADWEWVLGVDLWSVVYGVKAFLPLIREAGEGHIVNTASTAGLQASASIAPYNVAKF
                     GVVALTETLRAELDSEGSAISASVLCPGAINTQIVNSKRNRHSASAHDHQTSDAERAF
                     ESRAGRLLAEEGMDPAEVADMVVNAIRGDEFWIVTHPQWIDVLKSRVDAMARDRSLAR
                     GFGG"
     gene            4002..4436
                     /locus_tag="AC16VR_04365"
     CDS             4002..4436
                     /locus_tag="AC16VR_04365"
                     /inference="COORDINATES: protein motif:HMM:NF016955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HIR6010422.1"
                     /translation="MKDKILGTGFARFKDRRFLEASMAASALVAMADAHVHLSEQLAV
                     DRVLENIERLNVFDPRKAIDLHRQFVQAMRSNSGEGQDWALEVIGRFRGNEEAADLLI
                     RIARYTAQADQHLSGRELMAIRRLCRELGVDPEPYMEDADPD"
     gene            complement(4433..>5429)
                     /locus_tag="AC16VR_04370"
     CDS             complement(4433..>5429)
                     /locus_tag="AC16VR_04370"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR6010423.1"
                     /translation="AAIALKPLSLLGCNLTADQEIVTARGTVGETLRVPTANPRNFFE
                     AISDPGAMERTEILGKLFLPAGQGPWPVVVIVPGSLGIAPSHVAKADLLNEAGIAACL
                     IDPFGTRGVTSTVANQAQFSFAASAWDVLASVAVLAAREAIDAARIGAQGHSRGGSAV
                     LSAACMARLETVDVRSGLRAIYAAYPWSGQQFLSPFVGDTLVRAVIGDQDEWCLAQQV
                     QGHIQAIRLCGGDASCRIFAGAHHSFDRGTPLELIAEASVSPGAPTVYIREDGAFVHP
                     ADGRADPAFTDRELMMYGIKAGYGRRGAHLGSEGDQADRFHEDMMAFWIKALGVG"
BASE COUNT          985 a         1836 c         1695 g          913 t
ORIGIN      
        1 cgggcccacg tccggcgtcg agtccccgct cgatgagact gatgaggtga ttggtgtcga
       61 cgggtttgcg caggacggtg cagttggata tcgcggcctc gtcgatgact ttgccggagg
      121 tatcaccggt catcagcacc gtcggcagat agctgccccg cagctcgcgc gcgcgccgga
      181 tgacttcgat gccgttggcg cccggcaggc ggaagtcgga caccaccatg tcgggagaca
      241 gcccctcctc cagcatggcg atggccctgt tgccgtcgag cgccgagcgc accgagatcc
      301 cggcgagttc cagcatcatc gtcgtcgcgt ccacgatggc ggggtcatcg tctacgaaga
      361 ggatcaccgg ttcgccagga gccaactggc cggtctgcgg ttggcccggg accgtctcct
      421 cctcgtccag cagccccagg ggcaccagaa tgctgaaaca tgagccctcg cccacggtgg
      481 aagccacctc cagcgggcag tcgagcaagc tggctacctg tttcacaatg gacagaccga
      541 gacccagccc tttgcgccgg tcgcggaccg gattgtcgag ctggaaatac tcctcgaaga
      601 tggtgtcgat ggcttccccc ggaatgccga tacccgtgtc gcgcacctcg acgcgcaggt
      661 ggtctccact ctcggccgcg gagacggcga cgctgccttc ctccgtataa cgaaccgcat
      721 tgcccacgaa gttggccagg atccgctgca gcagcgctgg atccgtgcga acgacggcct
      781 cggtgggctc aatggatagc gtgaggcctt tgccctccgc ctcgggagca cagtctgcga
      841 ccagctcgtc gaacagactc tgaagccgga aatcccgcac ctctggcgta atcgagcccg
      901 agtccagctt ggagacgtcc agaagcgcat cgagcagctc acgcatcacg tccagagact
      961 tgtctatctt ggcgctgact tccaacgcct tgtcctggtc ctgacgcacc acccggttga
     1021 gcacgtcgag atagatggcg atggattgca ggggttgccg cagatcatgg ctggcagctg
     1081 ccaggaacct ggacttgatc gccgtggcgc gctccgcggt gtccttggcg gcactcagct
     1141 cgacctcgaa agcctttctt tccgtgacgt cgcgtatggc gctgatgacc agcataccct
     1201 ggtccgtgct gacagggctc aagctgatct cgatgggaaa ctccgagccg tcccgacgac
     1261 gcccgtagag gtcgagcccg gcacccatgg gccggacccg cggcgcagac atgaagtcgg
     1321 cgcgatgtgc gccatgggct gcacgcaacc gctcgggcag cagctggtcg acgttcagac
     1381 cgatgagggc ttccggcgcg taaccgagca cctcgccgac ctgcgcattc gcaaagacga
     1441 ttacgccagc cgaatccgcc accacggtcg cgtccggcgc tgcatcgagc agatcacgga
     1501 cgtgactttc cttgaaatcc atcgtctccc cctcaaatta cgctatcaga aacgcggcga
     1561 ggggagaagc gccacatggg gcctgaattc aagcctccgg ggtgcccgga gaaggaacca
     1621 aatcctggct ggacggtcca aatatgactt cccgtcaacc ggtccgaacc atggtcgtcg
     1681 atccgctgag gcgctaccct agccccatgc tgaagctcaa atgctccgtg cgtaaggccg
     1741 tgctgatcgg cgcgctggcc ggtgcgagcc ccgccatggc gggcgcgccg gaacaccaca
     1801 ccgaaaacgg ccgtttcacc aacccctacg tggaacaagc ccaagggggc gtttttgcgt
     1861 tccttaaacg gcgactttta ggacaggaca cgtggtccag ctacgacgcc catcgcgatg
     1921 gcgcagtgcc cacggctgca ccccgcctgg tcgccgaggg cgagcagacc aacaacgcca
     1981 gggtgacctg ggtcggtcat gccaccgtgc tgattcagca cgccggcgtc aacgtgctca
     2041 cggaccccat actttcgcga atcgcgtcgc ccgtcgcgtt cgcagggccg ggtcgtaagt
     2101 cggccccggg cgtagacgcc cagggccttc cccccatcga cgtggtggtc atctcccacg
     2161 atcactacga ccacctcgac gagcctacga tccgctttct gggcgaccgg ccccgatact
     2221 tcgttcctct gggactgaag cagtggctgg tggatgccgg tatcgatcaa tcgcgaatca
     2281 ccgagctgga ctggtgggag aagcagacgg tcgagcccgg cggctccgcg ttgacggtga
     2341 ccgccacccc tacccagcac gtctcgggac ggaccctgac cgatcgaaac aagcggctgt
     2401 gggccggctg ggcaatggag tgggacgact ttaccgtctg gtttggcggg gataccggat
     2461 acaacgacgt tcagttcaag gccatcggcg atcgtttcga cggcttcgac ctcggcatcg
     2521 tacccatcgg cagctacgcc ccgcgggcgt acatgcacag ctaccacgcc gaccctgagc
     2581 aggcagtgcg catccatcag gacatccgcg cgaggcggtc catggccgtg cactggggca
     2641 ccttccacct cactgccgag ccgctgctcg agccccccga gctgctgcgc gcagccgttc
     2701 tggaggcggg gctgcccagc gaagaggtca ccgcctacgc ggtgggcgaa tcccgcgcct
     2761 acttccccct ctgaacagtt cccagctgca cccgagcggc gaagcggtag cttcggccgc
     2821 cgtttttggt aggctagctt cgcagggcgc gtagctcagt tggttagagt gctgctttca
     2881 cacggcagag gtccgcagtt cgaatctgcg cgcgcccacc atctcgcccg ggttgccggc
     2941 cgaaccgtgc gctatgcagg ccggcgccac cgctggctca acctccaaaa cccctggcaa
     3001 gggaccgatc tcgggccatg gcgtccactc gcgacttgag cacgtcgatc cactgggggt
     3061 gcgtgacgat ccagaactcg tctccccgga tggcgttgac caccatgtcg gctacctccg
     3121 caggatccat gccctcttcc gccagcagcc gtccggcccg gctctcaaaa gctcgctccg
     3181 catccgaggt ctggtgatcg tgcgccgaag cgctatggcg gtttcgtttg ctgttgacga
     3241 tctgggtatt gatggcgccg ggacagagca cggaagctga gatggcggag ccttcggagt
     3301 cgagctccgc gcgcagggtt tctgtcaacg caaccacgcc gaacttggca acgttgtagg
     3361 gcgcaatgct cgccgaggcc tgcaggcctg cggtggatgc ggtattcacg atgtgcccct
     3421 cgcccgcttc ccggatcaac ggcagaaagg ccttgacgcc gtaaaccacg ctccacaggt
     3481 cgacgccgag aacccattcc cagtccgcca gggtgagatc ggcaatgggt ccggatacga
     3541 cgacgccggc gttgttgtgc accacgttga ccgagccgaa cgcatcgatg gtcttatcag
     3601 ccagcaggct gacggcttcc cagtcggaga cgtccgtagg cacgccgaga acctcgtgcc
     3661 cggctcccga aagctcggcc accgcacgct cgagggcggc ctcctcgatg tcggccatca
     3721 ccaccttcat gccttccccg gcaaagcgcg tagccgtcgc ccggccgatg ccgctggcag
     3781 caccggtcac caccgccacc tttccatcaa gttcacgcat ttcgttcccc ccagaagttc
     3841 gatccgcctg ccgcaagcat aaccggatcc agcgcttctc gtgcgctccg cgcaagcggc
     3901 tgtttcgcga atccgccccg ccctttatgc tgtccgcttg agaggcggcc ggaaccgggg
     3961 taccggtcgc aggcgcagca gacggggatc tccacatgag catgaaagac aagattctcg
     4021 gcaccggctt cgcacgcttc aaggaccgca gattcctgga ggcatccatg gcggccagcg
     4081 ccctggtcgc catggccgac gcccacgtac acctaagcga gcagttggcc gtggaccggg
     4141 ttctggaaaa catcgaacgt ctcaacgtct tcgacccgcg caaggccatc gacctgcacc
     4201 ggcagttcgt gcaggccatg cggtccaatt ccggggaagg ccaggactgg gccctggaag
     4261 taatcggccg gtttcggggt aacgaggaag ccgcagatct gctgatccgc atagcccgct
     4321 acacggccca ggccgaccag cacttgagcg gccgcgagct gatggcgatc cgccggctct
     4381 gccgggaact cggggtggat cccgagccgt acatggaaga cgccgacccc gactagccga
     4441 cgcccaacgc cttgatccag aaggccatca tgtcctcgtg aaaccgatcg gcctgatcgc
     4501 cctcgctgcc caggtgcgcg cccctgcgcc cgtagccggc tttgattccg tacatcatga
     4561 gctcacggtc ggtgaaggcg gggtcagcac gcccatcggc cgggtgcacg aacgcgccat
     4621 cctcgcggat gtagacggta ggggctcctg gagagacgga agcctcggcg atgagctcca
     4681 gcggcgtgcc ccggtcgaag ctgtgatggg ccccggcaaa aatgcggcag gacgcgtctc
     4741 ctccacacag tcttatcgcc tgaatgtgcc cctgcacctg ctgagccaga caccactcgt
     4801 cctgatctcc gatcacggcg cgcaccaagg tgtcgccaac gaacggggag agaaactgct
     4861 ggcctgacca ggggtaggct gcgtagatcg cgcgaaggcc ggacctgaca tcgaccgtct
     4921 cgagccgcgc catgcaggcc gcggacagca cggcggagcc accgcgactg tgtccctggg
     4981 cgccgatgcg cgcagcgtcg atcgcctctc tcgcggccag gactgctacg ctggccagca
     5041 cgtcccaggc actagccgcg aacgaaaatt gggcctgatt ggcgacggtg gaggtgaccc
     5101 cgcgggtgcc gaaaggatcg atcagacagg cagcgatgcc ggcctcgttg agcaggtcgg
     5161 ccttggcaac gtgggatggc gcaatgccca ggctgccggg aacgatcacc accaccggcc
     5221 agggcccctg cccggccggc aggaacagct tgcccagaat ctcggtgcgt tccatggccc
     5281 cggggtcgga gatggcctcg aagaagttcc gcgggttggc cgtagggacg cggagcgttt
     5341 cgcccacggt ccctcgggcg gtgacgatct cctgatcagc agtcaagtta caccccagca
     5401 gtgataaggg tttcagggcg atagccgcc
//
LOCUS       DBNHQR010000192         4155 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_82112, whole genome shotgun sequence.
ACCESSION   DBNHQR010000192 DBNHQR010000000
VERSION     DBNHQR010000192.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_82112"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="AC16VR_04375"
     CDS             complement(<1..616)
                     /locus_tag="AC16VR_04375"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
                     /protein_id="HIR6010424.1"
                     /translation="MTFTSLTSFREVDSELNWVIATENVARLRLSTGTVPLAVYGSND
                     YVDDSETFTQEFRLTSSGSGPFQWVGGLYYLNEETYRDETVPAGLLVPDGDGGALVLV
                     PAADAGDKQDNTTDSYAIFGQVTWDITDKLAITAGGRQDWEEKKISRLRTPNGLAPAR
                     IFDFSTSEDWSAFTAKGGIEFQATDDLFFYATVCKGFKSGGYQGS"
     gene            complement(671..955)
                     /locus_tag="AC16VR_04380"
     CDS             complement(671..955)
                     /locus_tag="AC16VR_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010425.1"
                     /translation="MKSQIDQFPKYFPGNSRSLLSNFDQHDIDSDSVRTGLRFLPSDR
                     LEINLTANYSTVDQAGPTRKTAGLYYTPDAALLPNYENAIHDNLVEDPGF"
     gene            1246..2553
                     /gene="aptA"
                     /locus_tag="AC16VR_04385"
     CDS             1246..2553
                     /gene="aptA"
                     /locus_tag="AC16VR_04385"
                     /EC_number="2.6.1.-"
                     /EC_number="2.6.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003290796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     S-adenosyl-L-methionine:8-amino-7-oxononanoate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0016223 - beta-alanine:pyruvate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="omega-aminotransferase AptA"
                     /protein_id="HIR6010426.1"
                     /translation="MSRADLENHWLPFTDNKTFKDDPRLVVRGEGAYLWNQRGEKLLD
                     GSAGLFTCALGHCRPEITEAVSAQLAELDYIPSFLRSHPRAFEAANLLTRILPDPINH
                     VFFCNSGSEAVDTAMKIVLQYQLARGQAQRSVFVTRDRAYHGVNLGGTALSGIARNRQ
                     AFGSLVPGIVHMRDTWDADQCFSRGQPARGGVDLADDLHRAVKTHGPNRIAACFVEPI
                     AGSTGVLVPPAGYLERLREICDEHDILLVFDEVITGFGRTGAAFGADSFGVTPDIMTM
                     AKALTNGAQPMGAVAVRDEIYQTVIDASPDDAVEFAHGYTASAHPAACAAAIATLGIY
                     EREKIFERAREMSEYFLDSVFALQNLPAVADIRGFGMLAGFDVAAEGTPGLRGMRLQR
                     ALFDAGLHVKTTGDAAILAPPLVIEKSQIDEMCETLRDVLKSI"
     gene            2603..3760
                     /locus_tag="AC16VR_04390"
     CDS             2603..3760
                     /locus_tag="AC16VR_04390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HIR6010427.1"
                     /translation="MSEEAEGVRVYRGLKGVHFDRSPTTYIDGRAGELRYWGYSIHEL
                     AEYSTFEETAYLLLHGELPTAAELAAFDGELKAARRLPDGLVDIIRAVQQAHPMDVLR
                     TAVSALAAFEPDVADTSRDGVLSKGIRLAAQVPAIVAAHHRLRSGLAPVEPDDDLGHT
                     AHFLYGLKGEVPSENAARLMDLDLILHAEHGSNASSFTARVVTGTDANLHAAITAAIA
                     ALSGPAHGGAAEDVMRMAREIGEPQNAAAYVKEKRGRREPIMGFGHRVYRAEDPRARH
                     LRSGVERLSEEMGEPHWNEILKAVVEAMQPYARLGVNVNVDFYSGVIYHLHGIPQDIF
                     VPIFAVGRVPGWTLQVLQQMESNILIRPLLEYNGPELRSYVPMDERSAAGS"
     gene            3757..>4155
                     /locus_tag="AC16VR_04395"
     CDS             3757..>4155
                     /locus_tag="AC16VR_04395"
                     /inference="COORDINATES: protein motif:HMM:NF013665.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HIR6010428.1"
                     /translation="MSFVETFLERARSLGRSVVFPEGEDPRILEAARCLADDGVAGIV
                     LLGSRDRIEAAAAEFQISLGGFRLIDPASGSVPQDYVDGYLANRPGARTGMAERAMRK
                     PLFFGAMMVKAGDADAMVAGAASPTRRVIEA"
BASE COUNT          688 a         1303 c         1403 g          761 t
ORIGIN      
        1 cgctaccctg gtagccgcct gacttgaacc ctttgcagac ggttgcgtag aagaacaggt
       61 cgtcggtcgc ctgaaactcg atgccgccct tagccgtgaa ggcgctccag tcctcgctgg
      121 tgctgaaatc gaagatccgc gccggcgcca ggccgttcgg cgtgcgcaac cggctgatct
      181 tcttttcttc ccagtcttga cggccaccgg cggtgatcgc cagcttgtcc gtgatgtccc
      241 aggtgacttg accgaatatg gcgtagctgt cggtggtgtt gtcctgcttg tcgcccgcat
      301 ccgcggccgg cacgagaacg agggcgcccc catcgccgtc gggcaccagc aggccggcgg
      361 gtacggtctc gtcgcggtag gtttcttcgt tcaggtagta gagcccgccc acccattgga
      421 aaggcccgct gccggacgag gtgagcctga actcctgcgt gaaggtttcc gagtcgtcaa
      481 cgtagtcgtt gctgccgtag acggcaagcg gcaccgtccc ggtggagagt cgcagccggg
      541 cgacgttctc ggtggcgatg acccaattca gctccgaatc cacctcgcgg aaggaggtca
      601 gcgaggtgaa cgtcatggtg tcgttgaaat cccagtcgat gcgccccgtg acgccccaga
      661 tgtcgctctt tcagaagccg gggtcctcca ccaggttgtc gtggatcgcg ttctcgtagt
      721 tcggcagcag cgcagcgtcg ggagtgtagt aaagcccggc ggtcttgcgg gtcgggccgg
      781 cctggtccac cgtgctgtag ttggcggtca ggttgatctc caggcggtcg ctagggagaa
      841 agcgcagacc ggtgcggacg ctgtcggagt caatgtcgtg ctgatcgaaa ttgctcagca
      901 gcgatcggct gttgcctgga aagtacttcg ggaactggtc gatctgcgac ttcatgtagc
      961 cgtcacgttt gcggctggag aaggttgctg ctatcgaaca ttgaacgttg tttttagcga
     1021 gccggtcctc cggctcctgt cgatgcggcg cactctgccg ggcttgaccc cgttcgtcca
     1081 gttgccctgt tgcggtcctt gaagcggcag gttccgctgt acagaccagc gcaagggtca
     1141 ttatatttcg cggttcgatg ggcctggctg gtgagtttcc gggggaagcg ataagtgagc
     1201 gaattactga atcagtccgg ggccggtgct cggccaggtc cggcgttgtc gagagccgat
     1261 ctcgagaacc actggctgcc gttcacggac aacaagacgt tcaaggacga cccgcgtctg
     1321 gtggtgcggg gcgaaggcgc ctacctgtgg aaccagcggg gcgagaagct gctggacggc
     1381 tccgcggggc tgttcacctg cgcgctcggt cactgccgac ctgagatcac ggaggcggtc
     1441 agcgctcagc tggccgagct cgattacatc ccgtcgttcc ttcgcagcca tcctcgggcg
     1501 ttcgaagcgg ccaacctgct cacccggatc ctgcctgacc ccatcaacca tgttttcttc
     1561 tgcaactcgg gttcggaagc cgtggatacg gccatgaaga tcgtgctgca gtaccagctg
     1621 gctagaggcc aggcccagcg tagcgtcttc gtcaccagag atcgggccta ccacggggtg
     1681 aacctggggg gcacggcgtt gagcggcatc gcccgcaacc gtcaggcgtt cggatccctc
     1741 gttccgggca tcgtgcacat gcgtgacacg tgggatgcgg accagtgctt cagcaggggc
     1801 cagccggccc gtggcggtgt ggatcttgcc gacgatctgc atcgagcggt gaagacccat
     1861 ggaccgaacc gcatcgccgc ctgcttcgta gagcccatcg cgggctccac cggcgttctc
     1921 gttccgcctg cgggttatct cgagcgcctg cgggagatct gcgacgagca cgacatcctg
     1981 ctggtgttcg acgaggtgat caccgggttc ggccgaacgg gggcggcctt tggtgcggat
     2041 tccttcggcg tcacgccaga catcatgacc atggccaagg ccctgaccaa cggtgcccag
     2101 cccatgggcg ccgtcgcggt gagagacgag atttaccaga cggtgatcga tgccagtccg
     2161 gacgacgcgg tggagtttgc ccacggctac acggcctcgg cccatcccgc ggcctgcgcc
     2221 gccgccatag cgaccctggg gatctacgag cgggagaaga tcttcgagcg tgcccgggag
     2281 atgtccgagt actttctgga ttcggtgttc gccctgcaga acctgccggc ggtggcggac
     2341 attcggggat tcggcatgct ggccggcttc gacgtggccg cggaaggcac gccgggactg
     2401 cgtggcatgc gtctgcagcg ggcgctgttc gacgcgggcc tgcatgtcaa gaccactggg
     2461 gacgcggcga tactggcgcc gccgctcgtc atcgagaagt cccagatcga cgagatgtgc
     2521 gagacattgc gagacgtgct caagtcgatc tgatgaggcc aagcgggatc gcctggacaa
     2581 ctcatgagcc gggggaggac ggatgagcga agaagcggaa ggcgtacgcg tataccgcgg
     2641 cctgaagggc gtgcactttg atcgctcgcc aaccacttac atcgacgggc gagcgggcga
     2701 gctgcgctac tggggttact ccatacacga gctcgccgag tactcgacgt tcgaggagac
     2761 ggcctacctg ctgctccacg gggagttgcc caccgccgcc gagctggccg ccttcgatgg
     2821 ggagctcaag gcggcgcgac ggcttcctga tggtctcgtc gacatcatcc gcgccgtgca
     2881 gcaggcacac cccatggacg tgctccgcac tgccgtgtcg gcgctggcag cattcgaacc
     2941 ggacgtggcg gacacatcga gggacggcgt cctgagcaaa ggcattcggc tcgccgccca
     3001 ggttccggca atcgtggccg cgcaccatcg cctccgatcg ggcttggcac ccgtggagcc
     3061 cgacgacgat cttggccata cggctcattt tctctacggg ctgaagggcg aggtgcccag
     3121 cgaaaacgcg gcccggctga tggatctgga tctcatcctg cacgccgagc atggctccaa
     3181 cgcctcgtcg tttaccgcac gagtggtcac cggcaccgac gccaacctcc acgcggcgat
     3241 cacggctgcc atcgcggcgc tctccggtcc cgcccacgga ggcgccgccg aggacgtcat
     3301 gcgcatggcc cgggaaatcg gcgagcccca gaacgccgcg gcctacgtca aggagaagcg
     3361 cggacgccgg gagcccatca tggggttcgg gcatcgcgtc taccgcgccg aggatccccg
     3421 ggcgcgacac ttgagatccg gcgtggagcg gctatccgag gagatgggcg agccgcactg
     3481 gaacgaaatt ttgaaggcgg tggtcgaggc catgcagcct tatgcccggc tgggcgtcaa
     3541 cgtgaacgtc gacttctact ccggggtgat ctaccacctc cacggcatcc cccaggacat
     3601 cttcgtgccc atcttcgccg tcggccgggt gccgggctgg acgctgcagg tgctccagca
     3661 gatggagagc aacatcctca tacgccctct gctcgagtac aacggaccgg agctgcgctc
     3721 ttacgtgcct atggacgaac gctccgcggc ggggtcgtga gcttcgtcga aacctttctc
     3781 gagcgcgcca ggtccctggg ccggagcgtc gtctttccag agggggaaga cccccgcatc
     3841 ctcgaagcgg cgcgttgcct ggcagatgac ggggtggccg ggatcgtgct gctcggcagc
     3901 cgcgatcgca tcgaggccgc ggccgcggag tttcagatct cgttgggcgg gttccgactc
     3961 atcgatcccg cgtccgggag cgtgccccag gattacgtgg acggttatct ggccaaccga
     4021 ccgggcgctc gcaccggcat ggcggagcgg gcgatgcgca agccgttgtt cttcggggcc
     4081 atgatggtca aggcggggga tgcggacgcc atggtcgcgg gagccgcctc gccgactcgc
     4141 cgcgtcatcg aggcc
//
LOCUS       DBNHQR010000193         4564 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_842918, whole genome shotgun sequence.
ACCESSION   DBNHQR010000193 DBNHQR010000000
VERSION     DBNHQR010000193.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4564)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4564)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4564
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_842918"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1393
                     /locus_tag="AC16VR_04400"
     CDS             <1..1393
                     /locus_tag="AC16VR_04400"
                     /inference="COORDINATES: protein motif:HMM:NF014543.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010429.1"
                     /translation="MRSDDGERYSGMWNEGRRHGHGELTNPNGDHYVGSFVNGLREGA
                     GTQKSQDGFYRGEWAGDLPNGEGRFNANDGSLYQGQWLDGERAGTGTYTDASGNRYEG
                     EWLADVPHGFGILTEQDGSEYDGRWTAGSRDGYGASRNALDMAYEGTWNAGNYHGFGI
                     LLRPDGSSYEGEWHHGKRQGQGRETDPEGGFHDGAWENNQPLGPGTRRDVAGVEIAGM
                     WTGSRVRTGLVKLPTGLEYAGPLFKDRNRGVSAPLQEWLLTQASRGDPYAQLYLGRAF
                     DDLGGTSADAARAAQWYALAAAAGVAEARYRLALLNIEDNPPRAVELLAAAAGQGHAR
                     ANAALADCYLAGKIVPRNAARAIAYLETAMDAGSVAARNTLARVLATTTEDGLRDGAR
                     AVALIRPLALYTESWWQLDTLAAALAETGAYGDAVTALQLALERADQISPRDVQQLVD
                     HLAAYRAETPLRE"
     gene            1398..2705
                     /locus_tag="AC16VR_04405"
     CDS             1398..2705
                     /locus_tag="AC16VR_04405"
                     /inference="COORDINATES: protein motif:HMM:NF012428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase family protein"
                     /protein_id="HIR6010430.1"
                     /translation="MTDLNWPFLGGAALEIESASGVHLTTRDGRRILDAAGGAIVVNI
                     GHGRRRVADRIAEAALTCSYVVPPWVTPSRRAMVEALEANWLDAAFTRLHFTSGGSEA
                     VEAAMKLALQYHAARGHGSRNQILSRAVSYHGTTVATTAVSGHAARRRGLEHGLPRPP
                     TIPTPDALRCPLGANHPDAGAYYAGAVREAIVEAGPENVAAFLAEPITGASGGAIVPP
                     ADYWPRVSEICREFGVLLILDEVMTGFGRTGTNFAYQQWEILPDILIAGKGLAGGYAP
                     LGGVFATEPVGAAIGAAGLQVMFHTFAAHPTACAAAAEVLDILVEEQLVARADRMGTM
                     LLERLQDAFREHPHVSDVRGRGLLQAIEIVSDRATLTPFPMEAQITRRVVDAALERGV
                     FFYAGGTGEMRDIICLGPPFVIKERHVDEMVSVLRQSVDAVTR"
     gene            complement(2827..3453)
                     /locus_tag="AC16VR_04410"
     CDS             complement(2827..3453)
                     /locus_tag="AC16VR_04410"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6010431.1"
                     /translation="MAADLNWLDSCRRLRAIAQTGLAYSRDPYDRDRFQELRLIAERM
                     LAWLSDAHPDRVAEVFLPERGYPTPKIDVRAGVFRGDSVLLVKERADGRWALPGGWGD
                     EGDSPRSSIEREVLEESGYEVRAARLVAIKDRHLHPYEKHSMLRVYKLFFLCDLLGGE
                     PRASLETELAEFFPITRLPELSLGRTLPDDVHLLNAYRAEPDRDVYLD"
     gene            complement(3443..4297)
                     /locus_tag="AC16VR_04415"
     CDS             complement(3443..4297)
                     /locus_tag="AC16VR_04415"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HIR6010432.1"
                     /translation="MTLLASSIARLDADARWALRGHELFFASLAVGEAQRAEGAAAEL
                     LASVEHGARALGVGKIGIDRPQDEALVAWYQQRGYYPGAYPRGYLGEGRGEGPALLAK
                     DLNAAREMEVFFRGWAPELIGTLLFVIDGSRVLLIDKKTGHGAGKINAPGGKLEPGES
                     PVACAVREVREEIGITVAAPRLHAELAFADLRGSQWFGYVFVARAYRGEPVETAEARP
                     SWHPLNAIPYGRMWEDDRVWLPRVVAGERVRGRFLFRAGRLLAHALEGLGEVDGSDAE
                     HRGGCHGR"
     gene            4400..>4564
                     /locus_tag="AC16VR_04420"
     CDS             4400..>4564
                     /locus_tag="AC16VR_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HIR6010433.1"
                     /translation="MTGIEVVEGDITTQSVDAIVNAANESLLGGGGVDGAIHRAAGPG
                     LKAECRRLGGC"
BASE COUNT          775 a         1567 c         1551 g          671 t
ORIGIN      
        1 catgcgcagc gacgacggtg agcgctacag cggcatgtgg aacgagggca gacgccacgg
       61 ccacggcgag ctgacgaacc cgaacggcga tcattacgtg ggcagctttg tcaacggcct
      121 gcgagaaggc gcaggaaccc aaaaatccca ggacggcttc taccggggcg aatgggcggg
      181 cgatctcccg aacggcgagg gccgcttcaa tgccaacgat ggaagcctct accagggcca
      241 gtggctcgac ggcgagcgtg ctggcaccgg gacgtacacc gacgcgagcg gcaaccgcta
      301 cgaaggggaa tggctcgccg acgtgcccca cggcttcggc attctcaccg agcaagacgg
      361 atccgaatac gacggccgat ggacggccgg aagccgcgac ggctatggag cttcgcggaa
      421 cgccctggat atggcctacg agggcacgtg gaacgcgggc aattatcacg gcttcggcat
      481 tcttctgcgc ccggacggca gcagctacga aggcgagtgg catcacggca agcggcaggg
      541 tcagggccgc gagacggacc ccgaaggagg cttccacgac ggggcctggg agaacaacca
      601 gcccctcggc ccgggtacgc gtcgcgacgt cgccggcgtg gagattgccg gcatgtggac
      661 cggcagccgg gtgcggaccg ggctcgtaaa gctgcccacc gggctggagt acgccggtcc
      721 gcttttcaag gaccgcaacc gcggcgtcag cgcgccgctc caggaatggc tgctcaccca
      781 ggcgtcgcgc ggtgacccct atgctcagct ctacctcggc cgcgcgttcg acgatctggg
      841 cggcacgtcg gccgacgcgg cccgggcggc ccagtggtac gccctcgcag cggcggcggg
      901 cgtcgccgag gcgcgctatc ggctggcctt gctcaacatc gaagacaacc cgccgcgcgc
      961 cgtcgagctg ctggccgcag ccgctggcca gggccacgcc cgggccaacg ccgcgctggc
     1021 cgactgttac ctggcgggca agatcgtccc cagaaacgcc gccagggcca tcgcctacct
     1081 ggagacggcc atggacgccg gcagcgtcgc ggcacgcaat accctcgcca gggtgctcgc
     1141 aaccaccacg gaggacggcc tgcgcgacgg cgcccgggcc gtcgcgctga tccgtccctt
     1201 ggcgctctac accgagagct ggtggcagct cgacaccctg gccgccgcgc tggcggagac
     1261 cggtgcttac ggggacgcgg tgaccgcgtt gcagctggcc ctggagcggg ccgaccagat
     1321 aagcccgcgg gacgtgcagc agctggtgga ccatctggcc gcctaccggg cggaaacacc
     1381 cttgcgagag tgagcccatg accgatctca actggccgtt ccttggcggc gccgccttgg
     1441 agatcgaaag tgccagcggc gtccatctga cgacccggga cggcaggcgt atcctcgatg
     1501 ccgccggcgg cgcgatcgtc gtgaacatcg gacacggccg acggcgggta gcggaccgca
     1561 tcgccgaggc ggcgctgacc tgcagctacg tggtgcctcc ctgggtaacg ccctcccggc
     1621 gtgccatggt ggaggcgctg gaagccaact ggctggacgc ggctttcacg aggctgcatt
     1681 tcacttccgg cggttccgag gccgtggaag ccgccatgaa gctcgcgctg caataccatg
     1741 ctgcccgagg ccacgggtca cggaatcaga tcctgtcacg cgccgtgtcc tatcacggga
     1801 ccaccgtcgc gactaccgcg gtcagcggtc acgcggcgag aagacgcggc ctcgaacacg
     1861 ggctgcctcg acctccgacg atcccgaccc ccgacgccct gcgctgcccg ctgggcgcca
     1921 atcatccgga cgcgggcgcc tactacgccg gagccgtgcg cgaggccatc gtcgaggcag
     1981 ggcctgaaaa cgtcgcggca ttcctggccg aacccatcac cggcgcgagc ggcggcgcca
     2041 tcgtgcctcc cgcggattac tggccgaggg tgagcgagat ctgccgggag ttcggcgtgc
     2101 tgctgattct ggacgaggtg atgacgggct tcggccggac gggaacgaac ttcgcctacc
     2161 agcagtggga gattcttccg gacatcctca tcgccggaaa ggggcttgcc ggcgggtatg
     2221 ctccgctggg cggtgttttc gccacggagc cggtgggcgc cgccatcggc gcggccgggc
     2281 tgcaggtcat gtttcacacc ttcgcagccc acccaaccgc gtgcgcggcc gctgccgagg
     2341 tgctcgacat cctggtcgag gagcaactgg tcgcgcgcgc cgatcgcatg ggcacgatgc
     2401 tgctggagag gcttcaagat gccttccgcg agcaccccca cgtgtccgat gtgcggggtc
     2461 ggggcctgct ccaggccatc gagatcgtca gcgaccgggc gaccctcacg ccgtttccga
     2521 tggaagccca gataacccgg cgggtggtcg acgcggcgct ggaacggggg gtcttcttct
     2581 atgccggggg taccggtgag atgcgcgaca tcatctgtct ggggcccccg ttcgtcataa
     2641 aggaaagaca cgtcgatgag atggtctcgg tgctgcggca gtccgtcgac gccgtgaccc
     2701 gataggcgac acgctcggcc cgttcgcgta tcagcagaag tgccggtcga tgtaacggac
     2761 cagacgcgcc ggcccgacta atttggagga gcaatcgcgc cggcgcacga gcgattaccc
     2821 atgggctcag tccaggtaaa cgtcgcggtc cggttccgcc cggtaggcgt tgagcaggtg
     2881 aacgtcgtcc ggcagcgttc tgccgaggct gagctccggc agccgggtga tcgggaagaa
     2941 ctcggccaac tcggtttcca ggctggcgcg aggctctccc ccgagcagat cgcacagaaa
     3001 aaagagcttg tacaccctga gcatcgagtg cttttcgtag gggtgcagat gccggtcctt
     3061 tatggcgacc agccgtgctg ccctgacctc gtagccggac tcctcgagca cctcgcgctc
     3121 aatggaggat ctgggcgaat cgccctcgtc gccccatccc ccgggcaggg cccaccggcc
     3181 gtcggcgcgc tccttgacca ggaggacgct gtcgccgcgg aacacgccgg cgcgcacgtc
     3241 gatcttcggc gtgggatagc cgcgttccgg caggaatact tcagcgacgc gatcggggtg
     3301 cgcatccgac agccaggcca gcatccgctc ggcgatcagg cgcagctcct gaaatcgatc
     3361 ccgatcgtag ggatcccgac tgtaagcgag tcccgtctgg gcaatggctc tcaagcgccg
     3421 gcagctgtcc agccagttca gatcagcggc catggcagcc tccccggtgc tcggcatcgc
     3481 tgccatccac ttcaccgagg ccttccagcg cgtgggcgag caaccgacca gcgcggaaga
     3541 ggaaccggcc acgaacgcgc tcgccggcaa ccacccgcgg caaccagacc cgatcgtcct
     3601 cccacatccg gccataggga atcgcgttca gcggatgcca gctcggcctg gcctcggctg
     3661 tctccaccgg ctctccccgg tacgcgcgcg cgacgaaaac gtagccgaac cactggctgc
     3721 cgcgcagatc ggcgaacgcg agttcggcgt ggagccgcgg cgctgcaacc gtgatgccga
     3781 tctcttcgcg aacttcgcgg actgcgcagg ccacagggct ttctcccggc tccagcttgc
     3841 cgcccggcgc gttgatcttg cccgccccgt gacccgtttt cttatcgatg agcagcaccc
     3901 gcgagccatc gatgacgaag agcagggtcc cgatgagctc aggcgcccac ccgcgaaaga
     3961 agacctccat ctcacgggca gcgttcagat cctttgctag gagagcgggg ccttcgcccc
     4021 gaccctcccc gaggtacccc cgtgggtagg cccctgggta gtagccgcgc tgctgatacc
     4081 aggcgacgag cgcctcgtct tgcggccggt cgattcctat cttgcccaca cccagagccc
     4141 gagcaccgtg ctccaccgaa gccagcagct cggcggcagc gccttccgcc cgctgcgcct
     4201 cgccgaccgc caacgaggcg aagaagagct cgtggccgcg cagcgcccaa cgggcgtccg
     4261 cgtcgagccg tgcgatggag ctggccagaa gcgtcatggc ccgccattgt cgacaggccg
     4321 ccggaagatg gcaattttac ccggggacgg gtgggggcta cccttcgggc gaagggcgac
     4381 agcttcgggc ggcatcgaca tgaccgggat agaggtcgtg gaaggcgaca tcaccactca
     4441 gtccgttgac gcgatcgtca acgcggccaa cgagtctctg ctcggcgggg gcggcgttga
     4501 cggtgccatt caccgggccg ccggccccgg tctgaaggcg gaatgccgca ggctcggcgg
     4561 atgc
//
LOCUS       DBNHQR010000194         2791 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_652609, whole genome shotgun sequence.
ACCESSION   DBNHQR010000194 DBNHQR010000000
VERSION     DBNHQR010000194.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_652609"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..1196)
                     /locus_tag="AC16VR_04425"
     CDS             complement(87..1196)
                     /locus_tag="AC16VR_04425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6010434.1"
                     /translation="MTSSPVNPLRSDEHPFKLAVFGINVSGGCTMTRAPGTLEVSWPE
                     TRQVALAAEQAGFDALIPVARWKGMGGAVNFNHRSWEPFTLASALAAITKRIALFSTC
                     HVPTVHPVRLAKESATVDQISGGRFCLNIVAGWNEREIGMFGVQQKPHDERYDVADDW
                     IHLTKRLWTETQEFDHQGPYFSAPGAYSEPKPVQAPHPLIMSAGNSPRGLAFAAEHAD
                     LNFVVAADLDKAGESASAVRAKAAEQGRSIKVFGQGYIVCRSSEAEARAARDRYVHEL
                     GDWEGVRNLLDVLIPNSQSALGDEWEAMAANMIAGYGALPLVGTPDQVVDGMQAFSDA
                     GLDGMTLSWLDYPAGIEQFEKELRPRLVEAGLRAR"
     gene            complement(1193..2182)
                     /locus_tag="AC16VR_04430"
     CDS             complement(1193..2182)
                     /locus_tag="AC16VR_04430"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6010435.1"
                     /translation="MSLASAQTIDVHAHAVLEETMGAAGEHGPELGREAAPRFRVGNY
                     ELHGVRYRGSPFMDPDLRLDAMDAAGIDYQVLSPNPLTYFHFIDAGAARDFCRIHNDT
                     LSAIVGERADRLGAFAALPMQDVDFAIEETERAVRELGMYGPYIGTDFGRPLNDPALD
                     PFYEKLIELDVPLFIHPAPAGLDGPPGDPNLKQFDLDIIIGFAAQETIATCTLIYGGV
                     LERHPKLDVCLSHGGGATGYAFGRMASAAKKRPWASDSLRQDGAFEALLQRLWFDVHV
                     HDPDALALLHKKVNPDHLVFGTNFAGWDQQPGDVRHDAAPYTENARRLLRATR"
     gene            2268..>2791
                     /locus_tag="AC16VR_04435"
     CDS             2268..>2791
                     /locus_tag="AC16VR_04435"
                     /inference="COORDINATES: protein motif:HMM:NF024375.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6010436.1"
                     /translation="MTSSDWGRVRPADIPWKQFDDPHGRPTTPVRVLKEGDPAVLEVR
                     FPPGYVAGLHWHPYDTLYIVTRGSMQIGDEGVYRPGDIRWVRAGHVYGPEAAGAEGVD
                     FFLVSLGGEIELNWADLVDVPERLRERLETSGEPWGRVNLQEVPWQDFEDPAGRPTQP
                     VQVLLDDDPYVLRTR"
BASE COUNT          455 a          889 c          943 g          504 t
ORIGIN      
        1 ggcctgggat tgctgatttt ttacctgccg ccgccgcacc gggcgctagc tctcgagccc
       61 atcgccgaga tcgctgcaca ggtgggttag cgggcgcgca acccggcctc gacgagccgg
      121 ggtcggagct ccttttcgaa ttgctcgatg ccggctggat agtcgagcca gctgagcgtc
      181 atcccatcga gccctgcgtc gctgaaggcc tgcatgccgt caacgacctg atccggcgtg
      241 cctaccagcg gaagcgcgcc gtagccggcg atcatgttgg cggccatggc ctcccactca
      301 tcgcccagcg cgctttgaga gttggggatg aggacgtcga gcaggttgcg gacgccttcc
      361 cagtcgccga gctcgtggac gtagcggtcc cgggctgccc tggcttcggc ctcgctggaa
      421 cggcaaacga tgtaaccctg gccgaatacc ttgatggaac gcccctgctc ggctgccttc
      481 gcccggaccg cgctggcact ttcccctgcc ttgtcgagat cggccgcgac gacgaagttg
      541 aggtctgcgt gctcggcggc gaatgccaga cctctggggc tgttcccggc gctcatgatc
      601 agagggtgag gcgcctgcac gggcttgggc tcggagtagg cgccaggtgc tgagaaatag
      661 ggaccctgat ggtcgaactc ctgagtttcc gtccacaatc gtttggtcag atgaatccag
      721 tcgtcagcga cgtcgtagcg ctcgtcgtgg ggtttctgct gcacaccgaa catgccgatc
      781 tcccgctcgt tccagcccgc tacgatgttc aggcagaacc tgcccccgga gatctgatcc
      841 acggttgccg attccttcgc cagccgcacg ggatgcacgg tgggcacgtg acaggttgag
      901 aagagcgcga tccgcttggt gatcgccgcc agcgcgcttg ccagcgtgaa cggctcccag
      961 ctgcggtggt tgaagttcac cgcgccgccc atgcccttcc accgcgccac gggtatcagc
     1021 gcatcgaacc ccgcttgctc ggcggccagc gccacctggc gggtctcggg ccagctcact
     1081 tccagggtgc ctggagcacg ggtcatcgtg cacccgccgc tcacgttgat gccgaacacg
     1141 gcgagcttga aggggtgctc gtcgctgcgc agcgggttga cgggcgagct ggtcatcgcg
     1201 ttgcgcggag caggcgacgc gcgttctcgg tgtacggcgc ggcgtcgtga cggacgtctc
     1261 ccggctgctg gtcccagccc gcaaaattgg tgccgaacac aaggtgatcg gggttcacct
     1321 ttttgtgcag aagggccaga gcgtccggat cgtgcacgtg aacgtcgaac cacagacgct
     1381 ggagcagagc ctcgaacgcg ccgtcctggc gcagcgagtc ggatgcccag gggcgctttt
     1441 tcgccgcgct cgccatcctt ccgaaggcgt accccgtggc cccgccacca tgactcaagc
     1501 agacgtccag cttcggatga cgctccagca ccccgccgta gatgagcgtg caggtggcaa
     1561 tcgtttcctg cgccgcgaag ccgatgatga tgtccagatc gaactgcttg aggttgggat
     1621 cccccggcgg gccatcgagg cccgcggggg cggggtggat gaaaagagga acgtccagct
     1681 cgatgagctt ttcgtagaaa ggatcgagcg cggggtcgtt cagcggccgt ccgaagtcgg
     1741 tgccgatgta gggaccgtac atgccgagct cgcgcaccgc ccgctccgtc tcctcgatgg
     1801 cgaagtccac gtcctgcatg ggcagcgcgg caaacgcgcc gagacggtct gcccgctcgc
     1861 cgacgatggc agacagggtg tcgttgtgga tcctgcagaa atccctcgcg gcgccggcat
     1921 cgatgaagtg aaagtaggtg agcgggttgg gtgacaggac ctggtagtcg attccggccg
     1981 cgtccatggc gtcgaggcgc agatccgggt ccatgaacgg gctgccgcgg tagcgcacgc
     2041 cgtgcagctc gtagttgccc actcggaagc gcggggcagc ctcgcgaccg agctccgggc
     2101 cgtgctcgcc cgcggccccc atggtctctt ccagaacggc gtgggcatgg acgtcgatgg
     2161 tctgggcgct cgcaaggctc atgtgtcgtt tcctcgggct gctgaagtgg gttcactcac
     2221 cgaaaccaga gcgatgatgg acccggtcgg atggtaagat caacgtcatg acgagttctg
     2281 attggggtcg cgtgcggccc gccgacatac cctggaagca gttcgacgat cctcacggac
     2341 gaccgaccac cccggttcgc gttctcaaag agggcgaccc cgccgttctc gaggtccggt
     2401 tcccgccggg ttacgttgcc gggctgcact ggcacccata cgacaccctc tacatcgtca
     2461 ctcgaggatc gatgcagata ggtgatgaag gtgtgtatcg cccgggagac attcgctggg
     2521 tacgggcggg gcacgtttac gggccggagg ccgccggcgc cgaaggcgtg gactttttcc
     2581 tggtgagcct cggcggcgag atcgagctca actgggccga cctggtcgac gtgccggagc
     2641 ggttgcgcga acgactggag acctcgggcg agccgtgggg gcgggtaaac cttcaagagg
     2701 tgccgtggca ggacttcgag gatcccgccg gacgacccac ccagccggtt caggttctcc
     2761 tggacgatga tccctacgtc ctgcgaaccc g
//
LOCUS       DBNHQR010000195         5334 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1665853, whole genome shotgun sequence.
ACCESSION   DBNHQR010000195 DBNHQR010000000
VERSION     DBNHQR010000195.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5334)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5334)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5334
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1665853"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..921)
                     /locus_tag="AC16VR_04440"
     CDS             complement(142..921)
                     /locus_tag="AC16VR_04440"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIR6010437.1"
                     /translation="MRLSADEAERYARDGFLIRECAFTEQELSSLRLAAERAADAAWR
                     LSRSGRTYVLDGNRFVDAGHVTVQFEHAAGSETIRVVEPVAELDADLNALVDDARICE
                     PMRDLVGSEEIALWTDKLNLKRPREGSGFGWHQDSPYWIHECRHVDHLPNVMVTLDDA
                     SEANGCFRVVRGSHKRGCLPGRADGTQLGGFYTDPDAFDARDQVPMVVAAGSLVFFSP
                     HSVHGSLPNRSDRPRRALVMTYQPAGHPMLKSGLVRNVMGG"
     gene            complement(945..2105)
                     /locus_tag="AC16VR_04445"
     CDS             complement(945..2105)
                     /locus_tag="AC16VR_04445"
                     /inference="COORDINATES: protein motif:HMM:NF024863.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR6010438.1"
                     /translation="MGSFTIAEIVPDRVCVRGALDFDHRPAGLSPRRLPAWTRPQLPR
                     EMDVMVRMPSGVRLAFTTDAAAIGLTFLATNMVMPPETRRPIPFDLEIDGALWSVASD
                     RGNTILLDRARPGAFELVKGDPDTVLFEDLPAGEKQCELWLPHNAYVELRELTLDDGA
                     FLAEPALDPRPRWVHYGSSISHCMEADHPGLIWPAVAARRAGASLTNLGFGGQCHLDQ
                     FVARTIREVDADVISIKTGINVINRDSMRERVFTPALHGFLDTVREGKPDTPIILISP
                     IYCPSAESRPGPTFPGRDGRFFTVSGHDEIREGCLTLRRVREIIEEVVEQRVAGGDRA
                     LAYVDGLDLFGAADAEDLPDDLHPNAAGYVRMGERFAPALADVLSSPETGGR"
     gene            complement(2113..2778)
                     /locus_tag="AC16VR_04450"
     CDS             complement(2113..2778)
                     /locus_tag="AC16VR_04450"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR6010439.1"
                     /translation="MEQLDLSNDDLLATTRAVRKRLDFDRPVEREALEECMELALQAP
                     TGSNAQGWHFVFVTDADKRGRIAELYQQAFSGYATSPMSAAAVHAGDPTMEQTQERVM
                     SSAQYLADNLHRAPVFLIPCIAGRFDGFSGEGANVAQASSFGSVIPAAWSFMLAARAR
                     GLGTCWTTLHLMHERKVADILGIPYEEITQVALIPVAYTKGTEFKRAPRKPMEGVVHF
                     ENW"
     gene            2926..3321
                     /locus_tag="AC16VR_04455"
     CDS             2926..3321
                     /locus_tag="AC16VR_04455"
                     /inference="COORDINATES: protein motif:HMM:NF024515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HIR6010440.1"
                     /translation="MRICGMGAAIFTLLLTGAASHAEMMAQSRAGHFLVTARSHLDPP
                     ATNRMHSWTLHVVDAAGQIVEDASIEVVGGMPDHDHGLPTAPRVTAYLGGGDYLLEGM
                     RFHMNGGWRIELTLRTDDVTDVAVFEIEL"
     gene            3336..4595
                     /locus_tag="AC16VR_04460"
     CDS             3336..4595
                     /locus_tag="AC16VR_04460"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004130 - cytochrome-c peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HIR6010441.1"
                     /translation="MNTAILLAACLAIGGLVYRLAEPTGARWSEAELAIIESLWLGNL
                     GEAPPDPTNEVADSLAAAALGHRLFFDTRLSRDGTVSCATCHKPESYFTDDLRVGIGA
                     GVGTRHTMTVVGAAWSPWYFWDGRRDSQWSQALTPLESPAEHAGTRTEFVHLLADDAG
                     YSAAYERIFGPLPDLTDAARFPRRASPLGTPEQQDAWWSMAPADQTAVNEAFANMGKA
                     LAAYQRELRPGPGRFDAYAQALASGEEPSGSAALSAAEVAGLRLFIGDASCVNCHNGP
                     LFSNNEFHNTGVLPGVGQLPAMGRASGVREARSDAFNCLGPFSADPLRTCAELRFAKS
                     SDDLVGAQRTPSLRNVSRTPPFMHAGQIASLGEVLEHYRRAEPSLVGHNEAKPLDLRQ
                     DQMRFLEAFLRTLDGDVAAEDRWLAPP"
     gene            complement(4606..>5334)
                     /locus_tag="AC16VR_04465"
     CDS             complement(4606..>5334)
                     /locus_tag="AC16VR_04465"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6010442.1"
                     /translation="STITLVPLYPPALLAKQVAALDVVSGGRFQLGVGVGGEFPREFE
                     ACGVPVSERGARTNEALEVMKRLWTEDDVRFEGPFTSLPGVTLNPKPTQKPHPAIWIS
                     GRSDAAMRRCARYGTGWLPYMYTPEMLHESLATIAQHSESVRRSEPVRPGLFIFFAVH
                     PDHDRAVAMAAERLSVQYNQDFTKLVGRYALAGDPAACIARLREYVDAGASTIILNSA
                     CPGDYVAENERLLAQEVLPALRAG"
BASE COUNT          880 a         1825 c         1773 g          856 t
ORIGIN      
        1 cctgccgccc acttgggctc gaagtcctcg ggaccgggca cgtcgaactc catctttcgt
       61 acggtgatgg gatgcatgag gctgtatcac ctgctccgga gagaacggga attatacacg
      121 cgggaacgaa cggtcatggg ctcatcctcc catgacgttg cgcaccagac cggatttcag
      181 catcggatgg ccggcgggct gataggtcat cacgagggct cgacgtgggc gatcggagcg
      241 gttgggcagc gagccgtgca ccgagtgggg gctgaaaaag accagcgagc cagcggccac
      301 caccatgggc acctggtccc tggcgtcaaa ggcgtccgga tccgtgtaga acccgccgag
      361 ctgggtgccg tccgcgcgac ccggcagaca tcctcgtttg tgacttcccc gcaccacccg
      421 gaaacaaccg ttggcctcgc tggcgtcgtc gagggtcacc atcacgttgg gcagatggtc
      481 gacgtgtcgg cactcgtgta tccagtaggg cgaatcctga tgccagccga agccggagcc
      541 ttcccgcggg cgcttgaggt tgagcttgtc cgtccacagg gcgatctcct ccgagccaac
      601 caggtcccgc atcggctcgc agatacgcgc gtcgtcgacc agggcgttca ggtccgcgtc
      661 gagctccgca acgggctcca cgacgcgaat cgtctcgctg ccggcggcgt gctcgaactg
      721 aacggtgacg tgtccggcat cgacgaaacg gttgccgtcc aggacgtagg tccttccgct
      781 gcggctcagg cgccaggcgg cgtccgccgc ccgctccgcc gctagccgca gtgacgagag
      841 ctcctgctcc gtgaatgcgc actcgcggat caggaaaccg tcacgcgcgt agcgctcagc
      901 ctcatcggca ctgagccgca agcccctctg actggacgcc ggcgtcaacg gccgccggtt
      961 tccgggctgc tcagaacgtc ggcgagcgca ggcgcaaaac gctcgcccat gcgaacgtat
     1021 ccagcagcgt tcggatgcag atcgtcgggc agatcctcag catcggccgc accgaagagg
     1081 tcgaggccgt caacgtaggc gagcgcccgg tcaccgccgg caacacgctg ctcgaccacc
     1141 tcctcgatga tctcccgcac ccggcgcagc gtcaggcaac cctcacgaat ctcgtcgtga
     1201 ccgctgaccg tgaagaaccg gccatcccgg ccggggaacg tgggtccggg ccgggattcg
     1261 gcgctggggc agtagatggg ggagatcagg atgatgggcg tgtccggttt tccttccctg
     1321 acggtgtcca gaaacccgtg cagcgccggc gtgaacaccc gctcgcgcat ggagtcccga
     1381 ttgatcacgt tgattccggt cttgatgctg atcacgtcag cgtccacttc acgaatggtc
     1441 cgggcgacga actgatccag atggcactgc ccgccgaaac ccaggttcgt caggctggcg
     1501 cccgcccgcc gcgcggccac ggcgggccag atcagtccag gatgatctgc ctccatgcaa
     1561 tgactgatgg agctgccgta gtgaacccag cggggacgcg gatcgagggc gggctctgcg
     1621 aggaacgccc cgtcgtcgag cgtcagctca cgcagctcca cataggcgtt gtgaggcagc
     1681 cacagctcgc attgcttttc acccgcgggc agatcttcga agaggaccgt gtcggggtcg
     1741 cccttcacga gctcgaacgc gccgggccgg gcgcggtcga gcagaatcgt gttgcccctg
     1801 tccgatgcga cgctccacaa cgcgccgtcg atctcgaggt cgaagggaat cgggcggcgc
     1861 gtctccggcg gcatcaccat gttggtggcc aggaaagtca ggccaatggc ggcggcgtcg
     1921 gtggtgaacg caagacgcac gcccgacggc atgcgcacca tcacgtccat ctcccgcggc
     1981 agttgcggcc gcgtccaggc cggcagtcgt cgcgggctga gaccggccgg cctgtggtcg
     2041 aagtccagcg cgccccggac gcatacccgg tccggcacga tctcggcgat ggtgaacgac
     2101 cccaagcgag cgctaccagt tctcgaagtg aacgacgccc tccatgggct tgcgcggcgc
     2161 ccgcttgaac tccgtgccct tggtataggc taccggtatg agcgccacct gggtgatctc
     2221 ctcgtagggg atgcccagaa tatccgcgac cttgcgctcg tgcatgaggt ggagcgtcgt
     2281 ccagcaggtt ccgagcccgc gggcccgcgc tgccagcatg aagctccacg ccgccggtat
     2341 caccgagccg aatgagctcg cctgggcgac gttggctccc tcgccggaga agccgtcgaa
     2401 acggccggcg atacagggga tcagaaacac cggcgcccgg tgcaggttgt cggcgagata
     2461 ctgcgccgaa gacatgacac gctcctgggt ctgctccatg gtcgggtctc cggcgtgcac
     2521 ggccgcagcc gacatcgggc tcgtcgcata accggagaag gcctgctggt agagctcggc
     2581 aatccggccc cgcttgtccg cgtcggtgac gaacacgaag tgccagccct gagcgttgga
     2641 tcccgtgggc gcctgcagcg ctagctccat gcactcctcg agcgcttcgc gctccacggg
     2701 ccgatcgaaa tccaggcgct tgcgcaccgc ccgggtggtg gcgagcaggt cgtcgttgct
     2761 gaggtcgagt tgctccatga cttggcattc tccggttcgt cgcgaaagac cctgctttat
     2821 gcggcgacgg ttttctccgg tcaagggcaa tcgcccgggc cgggcttttt ttgcgcgtct
     2881 tccccgcctg cgctacgctg ccgcagcatg tacttggagt tctcgatgcg catctgcggg
     2941 atgggcgcag ccatctttac cctgttgctg accggcgcgg cctcacacgc cgagatgatg
     3001 gcgcaatcca gagccggcca ttttctggtc actgcccgaa gccacctcga ccccccggca
     3061 accaaccgca tgcacagctg gaccctccat gtagtggatg cggcagggca gatcgttgag
     3121 gacgcctcga tcgaggtcgt cggggggatg cccgaccacg accacgggtt gcccacggcc
     3181 cctcgagtga ccgcctacct gggaggcggc gactacctcc tcgaaggcat gaggttccac
     3241 atgaacggcg gctggcgaat cgagctgacc ctgcgaacgg acgacgtcac tgacgttgcc
     3301 gtcttcgaaa tcgagctgta gacgtgcccg ggcgcgtgaa caccgccatc ctgctggccg
     3361 cctgccttgc catcggcggg ctcgtctacc ggctggctga gcccaccggt gcccgatggt
     3421 ccgaggcaga gctggccatc atcgagtcgt tgtggctggg caacctgggc gaggcgccgc
     3481 ccgacccgac aaacgaggtc gccgacagcc tggcagccgc tgcgctgggc caccggctct
     3541 tcttcgacac gcggctgagc cgcgacggca ccgtctcctg tgcgacctgt cacaagcccg
     3601 agagctactt cacggacgat ctgagggtcg gcatcggcgc cggcgtcggt acccgccaca
     3661 ccatgaccgt cgtcggcgcg gcctggagcc cgtggtactt ctgggacgga cgacgggaca
     3721 gccagtggtc ccaggcgctc acaccgctgg aaagtcccgc ggaacacgcg ggcacgcgaa
     3781 cggagttcgt ccacctgctc gccgacgacg cgggttactc cgccgcctac gagcggatct
     3841 tcggccccct tcccgacctg acggacgcgg cgcgttttcc ccgccgcgcg agcccgctgg
     3901 ggaccccgga gcagcaggac gcctggtggt ccatggcccc ggccgatcag acagcggtga
     3961 acgaggcttt cgccaacatg ggcaaggcgc tagccgccta ccagcgcgag ctgcgaccgg
     4021 gaccgggccg attcgacgcc tacgcgcagg cgctggcgag cggcgaggaa ccctccggca
     4081 gcgccgcgtt gtccgccgcc gaggtagcag gcctgcggct gttcatcgga gacgccagct
     4141 gcgtcaactg ccacaacggc ccgctgttca gcaacaacga gttccacaat accggcgtgc
     4201 tgccgggggt gggtcagctg ccggccatgg gccgggccag cggcgtgcgg gaagcgcgca
     4261 gcgacgcctt caactgcctg ggccccttca gcgccgaccc gcttcgtacc tgcgcggagc
     4321 ttcggttcgc gaagtcgagc gacgatctgg tcggcgccca gcgcacccct tctctgcgca
     4381 acgtgagccg gacgccgccc ttcatgcacg ccgggcagat cgcttcgctg ggcgaagttc
     4441 tcgagcacta tcgccgggca gagccgtccc tggtcggtca caacgaggcc aagcccctcg
     4501 acctcaggca ggaccagatg cgcttcctgg aggccttcct gcgaacgctg gacggtgacg
     4561 tcgcggccga agatcgctgg ctcgcgccgc cctaatcggg ctgagtcagc cggcgcgcag
     4621 ggcgggcaga acctcctggg cgaggagccg ctcgttctcc gccacgtagt ctcccgggca
     4681 ggccgagttc aggatgatgg tgcttgcgcc ggcgtccacg tactcacgca gccgggcgat
     4741 gcaggccgcc gggtcgccgg cgagggcgta acggccgacc agcttcgtga agtcctggtt
     4801 gtactgcacg gaaagccgct cggccgccat ggcgacggca cggtcgtgat cggggtgcac
     4861 ggcgaagaag atgaacaggc cgggccgaac gggctccgag cgccgcacgc tttccgagtg
     4921 ttgcgcgatg gtcgccaggc tctcgtggag catttcgggg gtatacatgt aaggcagcca
     4981 gccggtgccg tagcgggcac agcgacgcat ggccgcgtcg ctgcgcccgg atatccagat
     5041 ggcgggatgc ggtttctgcg tcggcttcgg attcagcgtc acccccggca acgaggtgaa
     5101 ggggccctcg aaccttacgt cgtcctccgt ccagagccgc ttcatgacct ccagcgcttc
     5161 gttggtgcgc gcgccccgct cgctcaccgg cacgccgcac gcctcgaact ccctgggaaa
     5221 ctcaccgccc acaccgaccc cgagttgaaa gcggccgccg gataccacgt ccagggccgc
     5281 cacctgcttg gcgagcagcg caggcgggta cagcggcacc agggtgatgg tgct
//
LOCUS       DBNHQR010000196         3563 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1222981, whole genome shotgun sequence.
ACCESSION   DBNHQR010000196 DBNHQR010000000
VERSION     DBNHQR010000196.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1222981"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="AC16VR_04470"
     CDS             <1..1107
                     /locus_tag="AC16VR_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009877130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR6010443.1"
                     /translation="AIEASGHRVVHFEVGEPDFVTADPIVRAGIDALEAGETKYTQAL
                     GIPELRERIAQHYCQELGVSLSADRVVVTSGASGGLLLLSGLLLNPGDRLLLTDPGYP
                     CNEVFAGLVNAEPERIPLSADRGFQLHADDLEAHWGPRARGLLLASPANPTGAVISRA
                     HLAELVAALARRGGWLILDEIYQGLIYGDGPGYRTGLEITDEAFILNSFSKYYGMTGW
                     RLGWMVVPEAALEPVARLAQNLFISPSSIAQRAALSAFGPEARAIHEQRRAAFEGRRD
                     ALREGLTELGLRVPVVPGGAFYLYVDVSPIGIDSAEFCWRLMDDYHVAVTPGIDFGDH
                     LADQYVRFAYTTDEDSIALGLERIETALKDMGAL"
     gene            1104..1805
                     /gene="sfsA"
                     /locus_tag="AC16VR_04475"
     CDS             1104..1805
                     /gene="sfsA"
                     /locus_tag="AC16VR_04475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HIR6010444.1"
                     /translation="MNFHPPLEVARLERRYKRFLADLLTDQGPLTVHCPNTGAMLGCS
                     DPGSEVWYSDSGNERRKYRHTLEVVCTPTGRVGINTARANRLVEEAIARGGVAELDGY
                     GQIRREAAIPESKGRFDFLLEGARGRCFVEVKSMTHNLGRGRGAFPDTVSERALRHVE
                     ALIGRKRAGDRAVLLFCVQHTGIDLATTADAIYPEYGEAVRRAVSEGVEVITYGCRID
                     RDALAVGEALPVELS"
     gene            complement(1798..2793)
                     /locus_tag="AC16VR_04480"
     CDS             complement(1798..2793)
                     /locus_tag="AC16VR_04480"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HIR6010445.1"
                     /translation="MYDIYGLGNALVDMEYRVDDGFLRRHAITKGHMTLVEEDRLDAL
                     IADLDDYDPERMSGGSAANTVIAAQCFGVSTFYSCKVAGDATGHYFLRDMGTAGVNTN
                     ANANDGPGKSGRCLVLVTPDAERSMNTFLGVSSQLSTAEIDEAALAKAGFFYVEGYLS
                     SSPGSLEAAVECRQLAEDSGVKTAVSLSDPSMVEFFRDGMEQILGNGVDHLFCNEEEA
                     LAWSRTDRLDIAIRELAEVARAVNITLGARGSLAVNGREQRRAPGYPVTAVDTNGAGD
                     IYAGACLFGWAAGMSSEQAAAFGNFAAANLVQRYGARFRHAGDYQSVLGDFRKVS"
     gene            complement(2808..>3563)
                     /locus_tag="AC16VR_04485"
     CDS             complement(2808..>3563)
                     /locus_tag="AC16VR_04485"
                     /inference="COORDINATES: protein motif:HMM:NF012428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR6010446.1"
                     /translation="EQTLTLPFNDLDAVAEAFEQYAGQIAAVILEPVAGNMGCVPPEA
                     GYLEGLRELTGRHVSLLIFDEVMTGFRVSRGGAQQRYGVEPDLTTLGKIIGGGLPVGA
                     FGGRADVMAHIAPEGTIYQAGTLSGNPLAMVAGLTTLRALDDPLYEELEQRTAYFCEA
                     LEASANRHGVSVTINYVCGMFSVFFTNGPVRNFADVARSDTASFNRFFHAMLDGGVYL
                     APSAFEAGFLSVAHTEDVLDATIEAADQAFATL"
BASE COUNT          576 a         1158 c         1180 g          649 t
ORIGIN      
        1 gccatcgagg cttcggggca ccgcgtcgtg cacttcgagg taggcgagcc ggacttcgtg
       61 accgccgacc ccatcgttcg ggcgggcatc gatgccctgg aggcagggga aacaaagtac
      121 acccaggcgc tgggcatccc ggagctgcgt gagcgcatcg cccagcatta ctgtcaggaa
      181 cttggtgttt ctctctccgc cgaccgggtc gtggtcacct cgggcgccag cggtggtctg
      241 ctcctgctga gcgggttgct gttgaacccg ggtgaccgcc tgctgctcac cgaccccgga
      301 tatccgtgca acgaggtgtt cgccgggctc gtcaacgccg agcccgagcg aattccgctg
      361 tccgctgacc gtggctttca gctgcacgct gacgatctgg aggcgcattg ggggcccaga
      421 gcgcggggtt tgctgctggc ttcgccggca aaccccacgg gggcggtgat ctcccgggcg
      481 catctcgcgg agctcgttgc tgccctggct cgtagaggcg gctggctgat cctcgacgag
      541 atctaccagg gcctgatcta cggtgacggg cccggatacc gcaccggtct tgagatcacc
      601 gacgaggcat tcattctcaa cagcttttcc aagtactacg gaatgaccgg ctggcggctc
      661 gggtggatgg tggttccgga ggccgcgctg gagcccgttg ccaggctcgc ccagaatctc
      721 ttcatctcgc cgtcgtccat cgcccagcgg gcggcattgt cggccttcgg gccggaggcg
      781 cgggcgattc acgaacaacg acgtgctgcc ttcgaggggc gcagagacgc gctgcgcgag
      841 ggcctgacgg agctcggtct gcgagtcccc gtggtgccgg gcggggcgtt ctatctctac
      901 gtggatgtct cgcccatcgg catcgactcg gccgagttct gctggcgcct gatggacgat
      961 taccacgtag cggttacgcc ggggatcgat tttggtgacc atctggccga tcagtacgtg
     1021 cggttcgcgt acaccaccga cgaagactcc atcgccctcg ggctcgagcg gattgagacg
     1081 gcgctcaagg acatgggcgc cctgtgaatt tccacccgcc gctggaagtt gctcgcctgg
     1141 agcgtcgcta caaaagattt ctcgccgacc tgttgaccga ccagggtccg ctgaccgtgc
     1201 actgccccaa caccggcgcc atgcttggat gctcagatcc cggcagcgag gtctggtatt
     1261 cagactccgg gaacgagcgc cgcaagtatc gtcacacgct cgaggtggtg tgcacgccga
     1321 ccggccgggt ggggatcaac acggcaagag ccaatcggct ggtcgaagaa gccattgccc
     1381 ggggcggcgt ggccgagctt gacgggtacg ggcagatcag gcgcgaagcg gccatccctg
     1441 aaagcaaggg tcgcttcgat ttcctcctcg agggcgccag ggggcgctgc ttcgtcgagg
     1501 tcaagagcat gacccacaat ctcggccggg ggcgcggcgc cttcccggat acggtgagcg
     1561 aacgggcgtt gcgccacgtc gaagcgctga tcggcaggaa gcgggccggt gaccgcgccg
     1621 tgctgctgtt ctgcgtccag cacaccggca tcgacctggc gaccacggcg gatgcgatct
     1681 atcccgagta cggtgaagcg gtgcgtcggg ccgtctccga gggtgtcgag gtgatcacct
     1741 acgggtgtcg gatcgatcgg gatgcccttg cggtgggcga ggcgctcccc gtggagctca
     1801 gctgaccttc cggaagtcgc caagcacgct ctggtagtcg cctgcgtgcc tgaatcgggc
     1861 accgtaccgc tgtaccaggt tagccgccgc gaagtttccg aaggcggccg cctgctccga
     1921 ggacatgccg gccgcccagc cgaacaggca ggcaccggcg tagatgtctc cggcaccgtt
     1981 ggtgtcgacg gccgtcaccg ggtagcccgg cgcgcggcgc tgctcacggc cgttcacggc
     2041 caggctgccc cgggcgccca gggtgatgtt cactgcccgg gccacctcgg cgagctcgcg
     2101 gatggcgatg tccagccggt ccgttcgcga ccacgccagt gcttcttcct cgttgcagaa
     2161 caaatggtcc acgccgttgc ccagaatctg ctccatgccg tccctgaaga actcgaccat
     2221 gctgggatcg gagaggctta ccgcggtttt cacgcccgag tcctcggcaa gctgccggca
     2281 ctccacggcc gcctccaggc ttcccggact ggagctcagg tagccctcga cgtagaagaa
     2341 gcctgctttg gccagtgccg cctcgtcgat ctcggctgtg ctgagctggc tcgacacgcc
     2401 gagaaaggtg ttcatggatc gctccgcgtc gggtgtgacc agcaccaggc aacgccccga
     2461 cttgcccggt ccgtcgttgg cattggcgtt ggtgttcacg cccgccgtgc ccatgtcccg
     2521 cagaaagtag tggccggtgg cgtcgccggc gaccttgcag gagtagaagg tgcttacgcc
     2581 gaagcactgg gctgcgatga ccgtgtttgc cgcggatcca ccgctcatgc gttcggggtc
     2641 gtagtcgtcc aggtcggcga tcagagcatc gagccggtct tcctccacca gcgtcatgtg
     2701 tcctttggtg atggcatgac ggcgcagaaa accgtcatcc acgcgatact ccatgtccac
     2761 cagggcattt cccaggccgt agatgtcata catgaacggg gctcccctta cagcgttgcg
     2821 aacgcctgat ctgcggcctc gatcgtggcg tccagcacgt cctccgtgtg ggcgaccgac
     2881 aggaagccgg cctcgaaagc cgagggtgcc aggtagacgc cgccgtcgag catggcgtga
     2941 aagaaccggt tgaaagatgc cgtgtcggaa cgggccacgt ccgcgaagtt cctgaccggg
     3001 ccgttggtga agaaaacgct gaacatgccg cacacgtaat tgatggttac gctcacgccg
     3061 tggcgatttg ccgatgcttc cagggcttcg cagaaatagg ccgtgcgctg ctccagttcc
     3121 tcgtagaggg gatcgtcgag cgcgcgcaag gtcgtcaggc ccgccaccat tgccagcggg
     3181 ttgcccgaga gcgttcccgc ctgatagatg gtgccttcag gcgcaatgtg cgccatgacg
     3241 tcggcgcgcc cgccgaacgc gcccaccggc agccccccgc cgatgatctt tccgagtgtg
     3301 gtcagatccg gctccacgcc atagcgctgc tgggcgccgc cgcgcgacac gcgaaatccg
     3361 gtcatgacct cgtcgaagat gagcaggctg acgtggcggc ccgtcagctc gcggagccct
     3421 tccaggtagc ctgcctccgg cggtacgcat cccatgtttc ccgccaccgg ctcgagaatg
     3481 accgcagcga tctgcccagc gtattgttcg aacgcttcgg ctactgcgtc cagatcgttg
     3541 aatggcagcg tcagcgtctg ctc
//
LOCUS       DBNHQR010000197         3392 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_145865, whole genome shotgun sequence.
ACCESSION   DBNHQR010000197 DBNHQR010000000
VERSION     DBNHQR010000197.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_145865"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="AC16VR_04490"
     CDS             complement(<1..600)
                     /locus_tag="AC16VR_04490"
                     /inference="COORDINATES: protein motif:HMM:NF015441.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HIR6010447.1"
                     /translation="MTVIGVVKEIWRFPVKSMQGSTIETCTISRTGIPGDRGWAMRDE
                     ERQEIQWGKRHPELMLCNASYRGEPEPGQTTPVDVTFPDGETLGSDDPGIHEKLSELV
                     GRRASLWPVQPPEDEAFYRRYKPDEEQFAREIAEVFAREPGEPMPDLSQFPPEILDYV
                     SVPGTFFDNEELNLVTTASIDHLRARNPAANWDIRRFRPN"
     gene            729..1919
                     /locus_tag="AC16VR_04495"
     CDS             729..1919
                     /locus_tag="AC16VR_04495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR6010448.1"
                     /translation="MTNDVYLAAGTRSALGNFGGSLKDVPMTELASHVARACIERSGA
                     NPDSVDHMVFTTTCPTDRDSLFSARVVGVKSGIPEMASALDVSRACASGLQAILSAGQ
                     QIESGQSRLALAGGSEMFSRVPYAVTTARWGIVRGNQQLEDMLEWCYRCPFSLEYMGE
                     TAENLTEEFQYDREAMDEYAVSSQARALAAIESGFLARQIVPVDVPDGRGTRTFEVDE
                     SPREGIDMERLARLKPAFREGGKVTAGNSSGVTDGAAAILVGSRGALESESVEPEARV
                     VDWAVVGVPPRIMGHGPVPAIQLLLERTGLSISDIDYFEVNEAFAVVNLHVERHLGIP
                     RDAHNLYGGGISVGHPPGVTGLRMAINAIQHLRETGGRYAILSMCMGGGQGMAMLIEN
                     AARH"
     gene            1931..2692
                     /locus_tag="AC16VR_04500"
     CDS             1931..2692
                     /locus_tag="AC16VR_04500"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6010449.1"
                     /translation="MDLGLAGKRAIVPGSTKGILRRVVDQLVDEGCDVAICSRSEDTV
                     ARALRELSGGRGKAIGRACDVMDKEDYEAWIAEMAESMGGADIFIPGVSAGGGSDSER
                     NWWKNFEVDVLSTVRGCEAVLPFMRQSGGGAITFVTTTASVETFGGPQAYNALKGSLL
                     VYAKQLSQAVGRDKIRVNCVSPGPIYFEGGVWAMLEDTMAKWFEKTVRDHPQGRLGAP
                     EEVANCILFLSSPAASWVSGTNLIVDGGFTKRVQF"
     gene            2706..>3392
                     /locus_tag="AC16VR_04505"
     CDS             2706..>3392
                     /locus_tag="AC16VR_04505"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6010450.1"
                     /translation="MEIRRKQVIERIDPKGQNVGSPADKAPDQDLGHELIPKQRYTSA
                     DFMRREWEGIWSRVWLLGCREEQIAEPGDFICTNIGKESVLLVRQADGGVRAFYNVCM
                     HRGNRLAEEGAGHAETFRCSYHDWQYGLDGRFVDIPDLNTFPQGAPPCAGLREIPCDT
                     WASFVWFSLNEDVEPLKDYMGDLVGHLEPYHFERMAMTRWVTAEWNCNWKASVDAFSE
                     SYHTARTHPQL"
BASE COUNT          580 a         1073 c         1139 g          600 t
ORIGIN      
        1 atttggtcga aagcggcgaa tgtcccagtt ggcagcaggg ttccttgccc ggagatggtc
       61 tatggaggcg gtggtgacga ggttcagctc ctcgttgtcg aagaacgtgc ccggtacgga
      121 gacgtagtcc agaatctcgg ggggaaactg gctcaagtcg ggcatcggct cgcccggctc
      181 gcgggcaaat acctcagcga tctcccgggc gaactgctcc tcgtcgggct tgtagcgccg
      241 gtaaaaggct tcatcctccg gcggttgaac cggccacagg gacgcccgcc tgccaaccag
      301 ctcgctgagc ttctcgtgaa tgcccgggtc gtcgctgccc agggtctcgc cgtcgggaaa
      361 agtgacgtcg accggagtgg tctgcccggg ctcgggttca cctcgatagg aggcgttgca
      421 cagcatgagc tcgggatgac gcttgcccca ctggatttcc tggcgctcct cgtcgcgcat
      481 cgcccagccc ctgtcgccgg gaatgcccgt cctggatatg gtgcacgtct cgatcgtgct
      541 cccctgcatc gatttgaccg ggaagcgcca gatctccttc accacgccga tgacagtcac
      601 tcgagatctc ccctctttgg atgcaccgca atcgtcgcac tgtggggtag tcgcgtccaa
      661 tctggatctc gggaacgttc tggcgggcgc tctggcgctg acctagaatg ccctgacggg
      721 agggagtgat gacaaacgac gtgtatctcg ccgccggcac ccgctcggcg ctgggcaatt
      781 tcggcggttc gctcaaagac gtgcccatga cggagcttgc ctcccacgtg gcgcgcgcct
      841 gcatcgaacg ttccggcgcg aacccggatt ccgttgacca catggtcttc accaccacct
      901 gccccacgga tcgggactcg ctcttctctg cccgcgtggt aggcgtgaag agcggcatac
      961 ctgagatggc gtcagccctg gacgtctccc gcgcctgtgc ctccggtctg caggccatcc
     1021 tctccgcggg tcagcagatc gagtcggggc agagccggct ggccctggca ggcgggtcgg
     1081 agatgttcag ccgggtgccg tacgcggtga ccaccgcccg ctggggcatc gtccggggca
     1141 accagcagtt ggaggacatg ctcgagtggt gttatcggtg cccgttcagc cttgagtaca
     1201 tgggcgaaac cgccgagaac ctcaccgagg aatttcagta cgaccgggaa gccatggatg
     1261 agtacgcggt gtccagccag gcgcgggctc tggctgccat cgagtcgggc tttctcgcca
     1321 ggcagatcgt gcccgtggat gttcccgacg gacgcggcac gcgcaccttc gaggtggacg
     1381 aatccccccg cgagggcatc gacatggagc gtcttgcacg tctgaagccc gcgttccggg
     1441 agggcgggaa ggtcacggcc ggcaactcgt ccggcgtcac ggacggtgcc gccgccatcc
     1501 tcgtgggcag ccgaggggcg ctcgagtccg agtcggtgga accggaagcc cgcgtcgtcg
     1561 actgggcggt ggtgggcgta ccgccgcgga tcatgggaca tggaccggtg cctgccattc
     1621 agcttctgct ggaacggacc gggctgagca tttcggacat cgactatttc gaggtgaacg
     1681 aggcgttcgc cgtggtgaac ctgcacgtgg agcgccacct gggaattccc agggatgctc
     1741 acaacctcta cggcggtggc atctccgttg gccatccccc gggggtcacc ggcttgcgca
     1801 tggccatcaa cgccattcag catctcaggg aaaccggcgg gcgctacgcc attctctcga
     1861 tgtgcatggg aggtggccag ggcatggcca tgctcatcga gaacgcggcc cggcattagg
     1921 agcgacgaag atggacctgg gattggcagg caagcgggcc atcgtgcccg gatcgaccaa
     1981 gggcatcctg cggcgggtcg tggaccagct cgtggacgag ggctgcgatg tggccatctg
     2041 ctcgcgctcc gaagataccg tggcgcgtgc cttgcgggag ctgtccgggg gcaggggcaa
     2101 ggccatcggc cgggcctgcg acgtgatgga caaggaggac tacgaggcct ggatcgccga
     2161 gatggcggaa tccatgggtg gtgcggacat tttcattcct ggcgtcagcg ccggtggcgg
     2221 cagcgacagc gagcgcaact ggtggaagaa cttcgaggtg gacgtcctct ctaccgttcg
     2281 cggctgcgag gcggtgctgc ccttcatgcg gcagtccggc ggcggagcga taacgttcgt
     2341 taccaccacg gcttcagtag aaacatttgg cggccctcag gcctacaacg cgctgaaagg
     2401 ctccctgctc gtctacgcca agcagctctc acaggcagtg ggccgcgaca agatacgcgt
     2461 caactgcgtc tcccccggcc cgatctattt cgagggcggg gtctgggcca tgctagagga
     2521 caccatggcc aagtggttcg agaagaccgt gcgcgatcac ccccagggtc gtctcggtgc
     2581 gcccgaggag gtagccaact gcatcctgtt tctgtccagc ccggcggcga gctgggtgtc
     2641 cggcacgaac ctgatcgtcg acggcggctt caccaaacgg gtgcagttct agagcgtccc
     2701 tgctgatgga gattcgccgc aaacaggtca tcgaacgcat cgacccgaaa ggacagaacg
     2761 tcggctcgcc cgcagataaa gccccggatc aggacctcgg ccacgagctg attcccaagc
     2821 agcgctacac gtccgccgat ttcatgcggc gggagtggga gggcatctgg agcagggtct
     2881 ggctgctggg ctgccgggag gagcagatcg ccgagcccgg cgacttcatc tgcacgaaca
     2941 tcggcaagga atcggtcctt ctggtccgcc aggccgatgg cggcgtccgg gccttctaca
     3001 acgtctgcat gcatcgcggt aatcggctgg ccgaggaggg agccggccat gccgagacgt
     3061 tccgctgcag ctatcacgac tggcagtacg gcctggacgg ccgcttcgtg gacattccgg
     3121 atctgaacac gttcccccag ggcgccccgc cctgtgccgg gcttcgcgag atcccctgcg
     3181 acacctgggc gagcttcgtc tggttcagcc tgaacgagga cgtcgagccg ctgaaggact
     3241 acatgggcga cctggtgggg cacctcgagc cctaccactt cgagcgcatg gccatgaccc
     3301 gctgggtgac cgcggagtgg aactgcaact ggaaggcgtc cgtcgacgcc ttctccgaga
     3361 gctaccacac ggcgcgcacc cacccccagc tg
//
LOCUS       DBNHQR010000198         5212 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1412517, whole genome shotgun sequence.
ACCESSION   DBNHQR010000198 DBNHQR010000000
VERSION     DBNHQR010000198.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5212)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1412517"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /gene="waaA"
                     /locus_tag="AC16VR_04510"
     CDS             complement(<1..1089)
                     /gene="waaA"
                     /locus_tag="AC16VR_04510"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF004388.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HIR6010451.1"
                     /translation="MWRILYQVLLPLAYPLVRLRLLWRARREPEYGRRIPERLGRVPA
                     SVPRGVIWFHTVSAGETIAAAPIIDRLLSEFPESDFLVTTMTPTGSQQVIARLGGRVA
                     HCYAPYDFRGGVERFLDAVAPRLLVIFETELWPNLLEAATRRGIPVDLVNARLSERSA
                     RGYARIGALTRRMLGQLQHIACQYPAHAQRFVALGAQPGRVSVLGSVKFDLRLPDDHR
                     ARCAELARRWRLEGRPVWIAGSTHPGEDAPVLAAHDAVRSACPGACLLLVPRHPSRAA
                     DLEKMIQEAGFSVVRQSVAAGAEAGVTVDVVIGDVMGQLLYLYGLSQAAFIGGRLVPV
                     GGHNPIEPALCGQPLLTGPHTFNFPDVVA"
     gene            1175..2344
                     /locus_tag="AC16VR_04515"
     CDS             1175..2344
                     /locus_tag="AC16VR_04515"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6010452.1"
                     /translation="MSGRDWQTDAIIVGGGIAGLWLANLLVRQGYQVVVLEKDRLGAG
                     QTLASQGMIHGGLKYALTGRLTRASQAIADMPERWRSCLDGTGDLDLSALAPLAERYY
                     LFAESSSLGRLTGFFASRSLRGRIEKVEPHDYPCVFANPAFHGVVYALRDFVLNTGAL
                     VSQLNDTLRGCVFQHAVAPEDLLVEADRVVLRLPDASLTARHLIICAGAGSQPLLDGL
                     GLRRPRMQLRPLHQVVVRHEHPHPMYAHCLTGIRRAEPRLTITSHPDGDHWLWYLGGQ
                     LATDGVKLDAPALIDRARRELEACVPWLSWDDARFETLRIDRAEPEQTRGGRPDEAFA
                     AAVGSCVVCWPTKLSLVPDLGDKVLGLLDPPSAPHPGSPDLGLPAARVGRSPWET"
     gene            2346..3104
                     /locus_tag="AC16VR_04520"
     CDS             2346..3104
                     /locus_tag="AC16VR_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6010453.1"
                     /translation="MQYRPLGNTGIEVPLIGLGTVKLGRDQGVRYPRPFTIPDARAAA
                     ALLKKARELGVNLLDTAPAYGTSEERLGALIGAERDHWIICTKVGEEFSNGVSRFDFS
                     PEHTRRSVERSLERLRTDRLDIVLIHSSGADVEILENLGTLDALRELQASGRVRAIGI
                     SHKTPDGGRLALAAGCDVIMTVLNPADREQAPIVAEAGRIGCGVLVKKALASGFAGPE
                     SLEWVAAQPGVSCIVTGTIDPEHLAANVAAVCPA"
     gene            complement(3141..4565)
                     /gene="hldE"
                     /locus_tag="AC16VR_04525"
     CDS             complement(3141..4565)
                     /gene="hldE"
                     /locus_tag="AC16VR_04525"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016495080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HIR6010454.1"
                     /translation="MNLPSLQGVHVLVVGDLMLDRYWIGPAERMSQEAPVPVVRVERV
                     EEHPGGAANVALNVASLGARCTLIGYVGKDDAAEALVDRLTAAGVACDLVVVDGWSTV
                     VKLRVVSQNQQLIRTDFESALIPGEDHRALLREKVIARLLDASAMVLQDYDKGVIEDP
                     ETLIGSAIAAGVPVVVDPKRKPFARYAGAAILKPNAKELEAALGSWDDDTDLVSLSAA
                     ACRTHRLGAMVITRGAGGMTVVAADGQHRHIPALPADVYDVTGAGDTAAAALAVTTSL
                     GWDADRGAQVANVASGLVVAKAGTAAITGPELALALAGRHRVDRGALRRAQLEDAVAQ
                     ARAAGERIVFTNGCFDILHAGHVAYLEEARALGDRLVVAVNDDDSVARLKGPGRPVNS
                     LERRLRVLAGLASVDWVVGFSEDTPEALLELLKPDVLVKGGDYGPSEVVGADQVIAQG
                     GEVRVLGLVEDCSTTAIVDRIRGA"
     gene            4629..>5212
                     /locus_tag="AC16VR_04530"
     CDS             4629..>5212
                     /locus_tag="AC16VR_04530"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015251.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR6010455.1"
                     /translation="MSRRRKSRPRMYSPRVWGTWLIVAIGWLLARLPISAIGGLGRWL
                     GRIAYRLARSRRHIAEVNLRLCFPDLGEAARREMIRGVFQHVAVGALETMVVWLDPDR
                     DLGDRVTIEGLEHLRAAQAEGKGVLLLGGHFTSLDVMANAVSRAVDLDVIYRANKNPV
                     WEWLQVRGRSRYCDGVIERSDVRQTIARLKAGRT"
BASE COUNT          855 a         1842 c         1730 g          785 t
ORIGIN      
        1 ggccaccacg tcagggaaat tgaacgtgtg gggccccgtc agcaggggct gaccgcacag
       61 ggccggctca atggggttgt gtccccccac aggcaccagc ctgccgccga tgaaagcggc
      121 ctgggacaaa ccgtacaggt agagcagctg ccccatgacg tcgccgatca ccacgtccac
      181 cgtcacccct gcttccgcgc cggcagcgac gctctgcctg accacggaaa atcccgcctc
      241 ctgaatcatc ttctccaggt ccgccgcccg gctcgggtgc cgcggcacca gcagcaggca
      301 cgcaccagga caagcgctgc gaaccgcatc gtgcgccgcc aggaccggcg catcctcacc
      361 gggatgagtg ctccccgcga tccagaccgg acggccttcc agccgccatc gacgggcgag
      421 ctcggcgcat cgggcgcggt ggtcgtcggg gaggcgcagg tcaaacttga cgctgccgag
      481 cacggacacc cgcccgggct gggcgccaag ggcgacgaat cgctgggcgt gggccgggta
      541 ctggcaggcg atgtgctgca gctgcccgag cattctgcgc gtcagggccc cgattcgggc
      601 atagccgcgc gcggatcgtt cagacagcct ggcgttgacc agatcaacgg gaataccccg
      661 ccgggtagct gcctccagga ggttgggcca cagctcggtt tcgaagatca ccagcagccg
      721 gggggcgacg gcgtcgagga agcgctccac gccgccgcgg aagtcatagg gcgcgtagca
      781 gtgagcgacc cgtccgccta ggcgggcgat gacctgctgg gacccggttg gcgtcatggt
      841 ggtgaccaga aagtcgctct cgggaaactc gcttagcaga cgatcgatga tgggcgcagc
      901 agcgatggtc tcgccggccg agacggtgtg gaaccagatg acgcccctgg gcactgatgc
      961 cggcacccgc cccaaccgct cggggatacg tcgcccgtac tcgggctcac ggcgcgcgcg
     1021 ccacagcagc cgcaaccgga ccagcggata ggccaggggg aggagcacct gataaaggat
     1081 ccgccacatg gcgcaagcct gcgcaggcgc gcggtccccg tcaagaatgc ccgggattgc
     1141 ggccctgcgg acgaaaccgt aggctcgcgc tcccatgagc gggcgagact ggcagacgga
     1201 cgcgatcatc gtcgggggcg gcatcgccgg cctgtggctg gccaacctcc tggtgcggca
     1261 gggatatcag gtggtagtac tcgaaaagga ccggctgggc gccggccaga cgctggcgag
     1321 ccagggcatg attcacggcg ggctgaagta tgcgctcacc ggccggctca cccgggcctc
     1381 gcaggccatt gccgatatgc ccgagcgctg gcgaagctgc ctggacggga cgggcgacct
     1441 ggatctttcc gcccttgctc ccttggccga gcggtattat ctgtttgccg agtcgtcgag
     1501 cctgggcagg ttgacggggt tctttgccag ccgctccctg cgcggacgga tcgagaaggt
     1561 cgagccgcac gattacccct gcgttttcgc aaaccccgcc ttccatggcg tcgtctacgc
     1621 gctgcgcgac ttcgtgctca acaccggcgc cctcgtctcg cagctgaacg acacgctgcg
     1681 gggatgcgtc tttcagcacg ccgtcgcccc ggaggatctg ctcgtcgagg cggatcgggt
     1741 ggtgctccgg ctcccggacg cgtcgttgac ggcgagacac ctcatcatct gcgctggcgc
     1801 cggctcccag ccgctgctcg acggcctggg gctgcggaga ccgcgcatgc agctgcgccc
     1861 gctgcatcag gtggtggtcc gacatgagca tccgcatccc atgtacgctc actgcctgac
     1921 cgggatccgc cgcgcggagc cgcgcctgac catcaccagc caccccgacg gcgatcactg
     1981 gctgtggtat ctcggcgggc agctggcaac ggacggcgtg aagctagatg cccccgccct
     2041 gatcgaccgg gcccggcgcg agctcgaagc ctgtgtcccc tggctttcgt gggacgatgc
     2101 gcgattcgag accctgcgca tcgaccgggc agagcccgag cagacccgag ggggtaggcc
     2161 cgacgaggcg ttcgcagcgg ccgtcggaag ctgcgtggtg tgctggccca ccaagctgag
     2221 cctggtaccc gatctcggcg acaaggtgct cgggctgctg gatccgccat cggctcccca
     2281 ccccggctcg ccggacctgg gcctgcccgc ggcccgggtc ggccgctcac cctgggaaac
     2341 ctagcatgca gtaccgtccc ctcggcaaca cgggcatcga ggtgccgctc atcggtctcg
     2401 gcacggtcaa gctgggccgc gaccagggcg tcaggtatcc gcgcccgttc accattcccg
     2461 acgcccgggc cgccgccgcc ctgctcaaga aagccaggga actgggcgtg aacctcctcg
     2521 acaccgcccc cgcctacggc acgagcgagg aacggcttgg cgcgctgatc ggtgccgagc
     2581 gggaccactg gatcatctgc acgaaggtcg gcgaggagtt ctcgaacggc gtgtcccgtt
     2641 tcgacttctc gcccgagcac acacggcgca gcgtcgagag gtctctcgag cgcctgcgca
     2701 cggaccgcct ggacatcgtc ctcatccact ccagcggagc ggatgtggag atcctggaga
     2761 acctgggcac gctggatgcg ctccgggagc tgcaggcttc gggccgcgtg cgagccatcg
     2821 ggatctccca caaaaccccc gacggcggcc ggcttgccct ggccgccggc tgcgacgtga
     2881 tcatgacggt gctgaacccg gccgaccgcg agcaggcccc catcgtggcg gaggccggac
     2941 gcatcggctg cggcgtgctc gtaaagaaag ccctggcaag cggcttcgcg gggcccgaat
     3001 cactggagtg ggtggcggca cagcccggcg tgtcctgcat cgtcaccgga accatagatc
     3061 cggagcacct ggccgccaac gtggccgccg tctgcccggc ttgaagccgg ccccgcgccg
     3121 gcacatcccg ggctcggcgc ctaagcgccc cggatccgat cgacgatggc agtggttgag
     3181 caatcctcca ccagaccgag cacgcgcacc tcgccgccct gggcgatgac ctgatccgcg
     3241 ccaaccacct cgctgggccc gtagtcgccc cccttgacca ggacgtccgg tttcaacagc
     3301 tccagcaggg cttcgggcgt atcctccgag aacccgacga cccagtcgac cgacgcgagc
     3361 ccggccagca cacgcaagcg tcgttcgaga gaattcaccg gccgacctgg gcctttcaga
     3421 cgcgccaccg agtcatcgtc gttgacggcc accaccaggc gatcgcccag cgcccgggct
     3481 tcttccagat acgcgacgtg gcccgcgtga aggatgtcga agcagccgtt ggtgaagacg
     3541 atccgctcgc cggccgctcg cgcctgagcg accgcgtcct ccagctgcgc cctgcgcaga
     3601 gcaccgcggt ccacccgatg acgcccggcc agcgcaagcg caagctcggg ccccgtgatc
     3661 gccgccgttc ccgccttggc caccaccagc ccgctcgcca cgttcgccac ctgggcaccg
     3721 cggtccgcat cccagcccag gctggtggtc acggcgagcg cggcagcagc ggtatccccc
     3781 gcgcccgtca cgtcgtaaac gtccgccggc aaggccggga tgtgccggtg ctgcccgtcg
     3841 gccgcaacga ccgtcatccc gccggcaccc cgggtgatga ccatggcacc caggcgatgg
     3901 gttctgcagg ccgccgccga caatgagacg agatccgtgt cgtcgtccca cgagccgagc
     3961 gccgcctcga gctcctttgc attgggcttg aggatggcgg ctcccgcata gcgggcaaag
     4021 ggtttgcgtt tagggtccac caccaccggc acgcccgccg cgatggcgga gccgatcagc
     4081 gtttcggggt cctcgatcac gcccttgtcg taatcctgaa gcaccatggc cgaagcatcc
     4141 aacagccggg caatgacctt ttcacgcagc agcgcgcgat ggtcctcgcc cgggatcaga
     4201 gcgctttcga aatcggtgcg gatgagctgc tggttctggc tgaccacccg cagcttgacc
     4261 acggtggacc aaccgtctac cacgaccagg tcgcaagcga ccccggctgc cgtgagcctg
     4321 tcgacgaggg cttcggccgc gtcgtcttta cctacgtaac caatgagggt gcagcgcgcg
     4381 ccgagactgg cgacgttgag ggccacgttg gccgcgcctc cgggatgctc ctctacccgc
     4441 tccaccctga ccaccgggac cggggcttcc tgagacatcc gctcggcggg accaatccag
     4501 tagcggtcca gcatgagatc gccgaccacc aagacgtgaa ccccttgcag cgacggcaga
     4561 ttcatggggc gtggatggta tcacagggcc tccccgttac aattcgccta ccccgccggt
     4621 ggaacctcat gagcaggcgc agaaagagcc gaccccgaat gtactctcct cgcgtctggg
     4681 ggacctggct catcgttgcc atcggctggc tgctcgcgcg tctgcccata tctgcgatcg
     4741 gggggctcgg gcgatggctg ggccgtatcg cctaccgcct cgcccggtca cgacgacaca
     4801 tcgccgaggt caacctcagg ctgtgttttc ccgatctggg agaagccgcg agacgggaga
     4861 tgatccgggg cgtgtttcag cacgttgcgg tgggcgccct ggagaccatg gtggtctggc
     4921 tggaccctga ccgcgacctc ggcgaccgag tgaccatcga aggcctcgag catctgcggg
     4981 ccgcgcaggc cgaagggaag ggcgtgctgc ttctgggcgg ccacttcacg tccctggacg
     5041 tgatggccaa tgcggtttcc cgcgccgtag acctcgatgt catataccgc gccaacaaga
     5101 atcccgtctg ggagtggctg caggttcgag ggcggagccg ctactgcgac ggcgtcatcg
     5161 agcgctccga cgtccgccag acgatcgcgc gtctcaaggc ggggcgcacg at
//
LOCUS       DBNHQR010000199         6323 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1602986, whole genome shotgun sequence.
ACCESSION   DBNHQR010000199 DBNHQR010000000
VERSION     DBNHQR010000199.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6323)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6323)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6323
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1602986"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..765)
                     /locus_tag="AC16VR_04535"
     CDS             complement(52..765)
                     /locus_tag="AC16VR_04535"
                     /inference="COORDINATES: protein motif:HMM:NF024937.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HIR6010456.1"
                     /translation="MEEYQAVVQTIALTMGVAWASGLNLYAALAVLGLTGSLGYVDLP
                     AQLEIVQDPLVILAAGFMYCVEFMADKIPGVDTGWDALHTLVRIPAGALLAAGALGDV
                     SPALAVTGGILGGGVAAATHATKAGSRALINTSPEPFSNWGASILEDLAVIGGLWAAL
                     THPMIFLAAFTIFMLLLCWLLPRIWRGVRRVFRTVGGWLGLVDASPANLQAELMKLGE
                     AGVLTAAEVHNARRRLHAA"
     regulatory      868..979
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1031..3127
                     /locus_tag="AC16VR_04540"
     CDS             1031..3127
                     /locus_tag="AC16VR_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6010457.1"
                     /translation="MNINTRGLLAGGLLLLAPVVSAQADEFVLIEEIVVTSEFRPTAL
                     EDTAASVSVISLADQKAGALNHLEEVVGWAPNVNLASGGGRARFVQIRGIGERGQFAE
                     PLNSSVGLLLDGVDMSGVGTVATLFDVEQVEILRGPQGTLYGANALAGLINVVSADPS
                     DELYTRLSLDAGNYGAFGVGGVISGPLGDNVGYRLAVQSYGDDGFMDNRFLDRDDTDD
                     HDELTIRGKLHWQVGPETDLRLTLGHVDVDNGYDAFSLDNDRNTLSDAPGQDRQQSTF
                     GSAQLNTALGEAVTLQATVGYADSDIDYGYDEDWTYAGFDPIGYTSEDRYRRDRQTLN
                     FDVRLLSGQAARMPGSVDWVLGVYGLHQEVDLERDYTFFFGEPFASRFEVDRLAVYGE
                     LGVQMSQRIRASLGLRFEHHESDYSDSEAVAADPKDDMVGGRLVLEADVADDAMVYFS
                     ASRGYKAGGFNTSGTLERELRDFDPEKLWNYEVGVKGTSADGRLQARVAAFLMKRDDV
                     QVSTFIAVPREDGSTEFIDLIDNAAEGTNAGLEAEVLWMPTEQLEVFASFGWLDTEFD
                     DFINAAGEDLDGEEQAHAPGYQFHVGGEYRFADGWFLRLAVEGRDAFYFTDGRRFAPK
                     AEDLKSDSYELVHASAGYEADRWRVSAWGRNLTDEDYAVRGFYFGNDPRDFYTARGFT
                     QLGEPRRYGITLQLEL"
     gene            complement(3154..3849)
                     /locus_tag="AC16VR_04545"
     CDS             complement(3154..3849)
                     /locus_tag="AC16VR_04545"
                     /inference="COORDINATES: protein motif:HMM:NF013488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HIR6010458.1"
                     /translation="MSSRKKSDKKVTRVRMLIVGFTILVGIVVLGYGTLYSTGLGEGE
                     FLAGEHYRVIENPPRRRAGAPIMVREYFSYGCIHCRNFEPLLDEWRATQPERTAFERS
                     PVAFSPAWQLLAQTYFTLKRLDVLEQNHMRLFRAIHDNARQFATPESMADFVDGNGVT
                     RAEFLRMFNSPEVRGDLREADAIQRNIVISSVPTLVVDDRYVVNMDLGRKRALDVVDY
                     LISQQLAETAAAP"
     gene            3983..4240
                     /locus_tag="AC16VR_04550"
     CDS             3983..4240
                     /locus_tag="AC16VR_04550"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024809.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HIR6010459.1"
                     /translation="MTESYQLFQYDQCPFCRRVRQFMDAAGIDIEIRDTLRDPEALRE
                     LIRGGGRSMVPCLKIQSEEGTRWLYESADIITYLRDRFAAG"
     gene            4345..5385
                     /locus_tag="AC16VR_04555"
     CDS             4345..5385
                     /locus_tag="AC16VR_04555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6010460.1"
                     /translation="MKLGILSGYSGRKMNIPIDAIRHAESLGYESIWTAEAYGSDAVT
                     PAAWILAQTQKIKVGTAIMQMPARTPAMTAMTAMSLAELSGGRFICGVGASGPQVVEG
                     WHGVPYGLPVTRLKEYVQIMKRIFAREAPLEFEGRMYQLPYQGPGATGLGKPLKSILH
                     CEEDIPIYAATITPKGVQAAAEVADGFFPVWMDPEQYHVFEEPVAKGFASAGGDKDLT
                     RFDIAPFVTVVMGDDVEQCMMPIRGSMALYIGGMGARGKNFYNDYAKRLGFEEAAVRI
                     QDLYLEGRKDEAMAAVPAELIDACHLVGPADRIRERLQRWQAAGRQGHVSSMLLGCQQ
                     PEALALVAETVL"
     gene            complement(5387..>6323)
                     /locus_tag="AC16VR_04560"
     CDS             complement(5387..>6323)
                     /locus_tag="AC16VR_04560"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HIR6010461.1"
                     /translation="VRQFEGGQSNPTFQLEAGGRLYVLRKQPPGELLPSAHQVDREYR
                     VMKALEQTEVPVPLMKCLCEDAGVIGTKFYVMEMVNGRVFAETLLPSLTPPERRAIYL
                     DLARVLAKLHSVDPAAVGLADFGRPGNYYERQIGRWTKQYEASKTEQIDAMDRLMAWL
                     PERIPEPGETVIVHGDYRLGNALIHPTQPRIVAVLDWELSTLGDALADLGYICQDYHG
                     DSYNDAGLSGADLGALGIPTEDELVAEYCRHAGREHIDNWPFYVIYNMFRSASIIQGV
                     YKRGLDGNASSEKALEYKDAARIRSEQAWALVESL"
BASE COUNT         1092 a         2013 c         2087 g         1131 t
ORIGIN      
        1 gggaaatccg ttcagccatc gaggcgcggg atcacgaccg gcggcccgat gtcacgcagc
       61 gtgaagcctg cgccttgcgt tgtgaacttc cgccgcggtg agaacgcccg cctcgcccag
      121 cttcatcagc tccgcctgca ggttggcggg cgaggcgtcg accaggccca gccagccgcc
      181 caccgtgcgg aacacgcgcc gcaccccacg ccatatcctg ggtaacaacc agcatagcaa
      241 caacatgaaa atcgtgaagg cggccaagaa tatcatgggg tgcgtgagcg ccgcccagag
      301 gccgccgatc acggcaaggt cctcgaggat ggaagcgccc cagttgctga acggctcggg
      361 ggaggtgttg atcagcgccc gcgaccccgc cttggtggca tgggtcgccg cggccacccc
      421 gccaccgagg atgcctccgg tcacggccag tgcgggcgac acgtcgccga gcgcgccggc
      481 tgccaggagg gcgccggcgg gaatcctgac cagggtgtgc agcgcatccc accccgtgtc
      541 cacgccgggg atcttgtcgg ccatgaactc cacgcagtac atgaacccgg cggccagtat
      601 caccaggggg tcctggacga tttcgagctg cgccggaagg tccacgtagc ccagtgatcc
      661 ggtaaggccg agcacggcca aggccgcgta gaggttcagc ccgctcgccc acgccacgcc
      721 catggtcagg gcgatggtct gcacaactgc ttgatattct tccatttcgc ttcggatctg
      781 cggtttctca gcgtcactct acgtggcgaa tctgaaccct aactgaagca cgcgcttgcg
      841 cccgcaagga ccccgggcga gaatctctgc agacctaagg ggcggtactg cctgagacac
      901 cggtcgagcg cggctcgtcg gtggaccctt tgaacctgat tcggttaatt ccggcgtagg
      961 aataggcact tctcttctcc gtcatttcac cccttcgcgg tcgcaacaag gcaacctgag
     1021 tgggtggacc atgaacatca atacccgagg actgctggct ggcggcttgc tgttgctggc
     1081 accggtggtt tcggcacagg ccgacgagtt cgttctcatc gaggagatcg tcgtaaccag
     1141 cgagttccgc cccacggcgc tggaggacac cgcggcgagc gtgtcggtga tctccctcgc
     1201 cgaccagaaa gcaggggctc tcaaccatct cgaggaggtt gtggggtggg cgcccaacgt
     1261 gaatctggcc agcggcggcg gccgggcccg attcgtgcag atccgcggca tcggtgagag
     1321 gggccagttc gccgagccgc tcaactcgtc ggtagggctt ctgctcgacg gcgtagacat
     1381 gagcggggtc ggcaccgtcg ccacgctctt cgatgtggaa caggtggaaa ttctgcgcgg
     1441 gccccagggg acgctctacg gggcgaatgc cctggctggc ctcatcaacg tggtcagcgc
     1501 cgaccccagc gacgagctct acacgcggct ctccctggat gccggcaact acggggcgtt
     1561 cggggtgggt ggcgtgatct ccggtcccct gggggacaac gtcggctatc gcctggccgt
     1621 gcagagctac ggtgacgatg gcttcatgga caaccgcttt ctggatcggg acgacacgga
     1681 tgaccacgac gagctgacca tccgcggcaa gctccattgg caggttggcc cggaaaccga
     1741 tctgcgactc acgctgggcc acgtggacgt ggacaacggc tacgatgcgt tctcgctcga
     1801 caacgaccgc aacacgcttt ccgatgcccc gggccaggac cgccagcaga gcacgttcgg
     1861 cagcgctcag ctgaacaccg cgctgggcga ggccgtcacc ctacaggcta ccgtgggcta
     1921 tgcggacagc gacatcgact acggttacga cgaggactgg acctacgcgg gtttcgaccc
     1981 catcggctac acctccgaag accgctaccg gcgcgatcgc cagacgctca acttcgatgt
     2041 ccggctgctt tccgggcagg cagcccgaat gcccgggtcc gtcgactggg tgctgggcgt
     2101 gtacggcctg catcaggaag tggacctcga gcgcgactac accttcttct tcggcgagcc
     2161 gttcgccagc cgtttcgagg tggaccggct ggccgtctac ggcgagctcg gcgtgcagat
     2221 gtcgcagcgc atccgggcct ctttggggct tcggttcgag caccacgagt ccgactactc
     2281 ggattccgag gcggtggcgg ccgatccgaa ggacgacatg gtcggcggtc ggttggtgct
     2341 ggaagcggac gtcgcggacg acgccatggt ctatttcagc gccagccgcg gctacaaggc
     2401 cggcggcttc aataccagcg gcaccctgga gcgcgagctg cgggatttcg atcccgaaaa
     2461 gctctggaac tacgaggtgg gcgtgaaggg gacgtcggcc gacggccggc tccaagcgcg
     2521 cgtcgcggcg tttctgatga aaagggacga cgttcaggtg tccactttca tcgccgtgcc
     2581 ccgcgaagac ggcagcaccg agttcatcga cctcatcgac aacgcggccg aaggcaccaa
     2641 cgccgggctg gaagccgagg tcctctggat gcccaccgag cagctagagg tgtttgccag
     2701 cttcggctgg ctcgataccg agttcgacga tttcatcaac gccgcgggag aggatctcga
     2761 tggcgaggag caggctcacg cgcccggcta ccagtttcac gtcgggggcg agtaccgatt
     2821 cgcggacggc tggttcctgc gcctggcggt ggagggcagg gacgcttttt acttcaccga
     2881 cggccgccgg ttcgcgccca aggccgaaga tctgaagtcg gacagctacg agctggtgca
     2941 tgcctctgcg ggttacgaag cagaccggtg gcgggtgtcc gcctggggcc gcaatctgac
     3001 ggacgaggac tacgccgtac gaggcttcta tttcggcaac gatccgcggg atttctacac
     3061 ggcgcgcggc ttcacccagc tcggcgagcc tcgccgttac ggaatcaccc tccagctgga
     3121 gctctgaccg tcgtcaatcc cgtgcaggcc gcgtcagggc gcggcggcag tctctgcaag
     3181 ctgctgggag atcagatagt cgaccacgtc gagcgctctt ttgcgcccca ggtccatgtt
     3241 caccacgtac ctgtcgtcca cgaccagggt ggggacgctg gagatgacga tgttgcgctg
     3301 gatggcgtct gcttcgcgca ggtcgccgcg cacttccggt gagttgaaca tccggagaaa
     3361 ttcggctcgg gtaacgccat tgccgtctac gaaatcggcc atggattccg gcgttgcgaa
     3421 ctgccgagcg ttatcgtgaa tggctcggaa cagacgcatg tggttctgct ccagcacgtc
     3481 caggcgcttc aacgtgaaat aggtctgcgc cagcagctgc caggccggcg agaaagccac
     3541 tggtgagcgc tcgaaggcgg tccgttccgg ctgcgtcgct cgccactcgt cgagcagcgg
     3601 ctcgaaattc ctgcagtgga tgcagccgta ggagaaatac tcgcgcacca tgatgggcgc
     3661 gcccgcccgc cgtctgggcg ggttctcgat gacccggtaa tgctcgccgg ccagaaactc
     3721 gccttcgccc aggccggtgc tgtagagcgt gccgtacccc agcaccacga tgccgaccag
     3781 gatggtgaag ccgacgatca gcatgcgcac ccgggtcact ttcttgtccg actttttgcg
     3841 actggacatg gggcctcccc tgtcgtttgc gccggagttt acccacagcg acgctggtgt
     3901 ggaaactcgg gtagcgcgcc ctttgcatga ctttcggtgg cggtggcata ctgcgccgct
     3961 ttttttcggc gagcggaatc ctttgaccga atcctatcag ctgttccagt acgatcagtg
     4021 cccgttctgc cgtcgggtcc gtcagttcat ggatgcggcc ggcatcgaca tcgagatacg
     4081 cgacaccctt cgtgatcccg aagcgctccg cgagctgatt cgcggcggcg ggcggtccat
     4141 ggttccctgc ctgaagattc aatccgagga aggcacccgc tggctctacg agagcgcgga
     4201 catcatcacc tacctgcggg accgcttcgc agccggctga gcggtctcgg ggctcgcgtt
     4261 gccgtgggtt gggcggtctg ttaacttgct gcgccttcgg cgggcgcggt cccgccagaa
     4321 actacacacc tgggggctcc tcatatgaaa ctcggaatcc tttccggcta ttccggacgc
     4381 aagatgaaca ttcccatcga cgcgatcaga cacgccgaga gtctaggcta cgaatccatc
     4441 tggaccgccg aggcctacgg ctcggacgcc gtgacgccgg cggcctggat cctggcgcag
     4501 acgcagaaga tcaaggtggg cacggccatc atgcagatgc cggcacgcac gcctgccatg
     4561 acggcgatga cggcgatgag ccttgccgag ctgtcgggcg gtcgtttcat ttgcggcgtc
     4621 ggtgcttccg gaccccaggt cgtggaaggc tggcacggtg ttccctacgg gcttccggtg
     4681 acgcgcctga aggaatacgt gcagatcatg aagcgcatct ttgcccggga ggcgccgctc
     4741 gagttcgagg ggcggatgta tcagctgcct tatcagggtc cgggtgccac gggtctgggc
     4801 aaacctctga agagcattct tcactgcgag gaggacatcc ccatctacgc cgcgaccata
     4861 acgcccaaag gcgtgcaggc agccgcggag gtggcggacg gcttcttccc cgtgtggatg
     4921 gatcccgagc agtaccacgt cttcgaagag ccggttgcca agggcttcgc cagcgctggc
     4981 ggggacaagg atctgacccg gttcgacatc gcacccttcg tcaccgtggt gatgggcgac
     5041 gacgtcgagc agtgcatgat gcccatacgc ggcagcatgg cgctctacat cggcggcatg
     5101 ggcgcccgcg gcaagaactt ctacaacgac tacgccaagc ggctgggttt cgaggaggct
     5161 gcggtcagga tccaggacct gtacctcgaa gggcgcaagg acgaagccat ggccgcggtg
     5221 cccgcggagc tcatcgacgc ctgtcacctg gtggggccgg cggatcggat tcgcgagcgg
     5281 ttgcagcgct ggcaggcggc ggggcgtcag gggcatgttt ccagcatgct gctgggttgc
     5341 cagcagccgg aggcgctggc gctggtggcg gaaaccgtcc tctagcttag agcgattcca
     5401 ccagggccca ggcctgctcc gatcggatgc gggccgcgtc cttgtactcc agcgctttct
     5461 cggagctggc gttgccgtcc aggccgcgct tgtacacgcc ctgaatgatg ctcgccgagc
     5521 ggaacatgtt gtagatcacg taaaacggcc agttgtcgat gtgctcgcgg cccgcgtgcc
     5581 ggcagtactc ggccacgagc tcgtcctcgg tggggatgcc gagcgcgccc aggtcggcgc
     5641 cggaaagacc cgcatcgttg taggaatcgc cgtggtagtc ctggcagatg tagccgagat
     5701 cggcgagcgc gtcccccagc gtcgacagtt cccaatccag aaccgccacg atgcggggtt
     5761 gcgtagggtg gatcagcgcg ttgcccagcc ggtagtcgcc gtgcacgatg acggtctccc
     5821 ccggttccgg gatgcgttcg ggaagccacg ccatgagcct gtccatggcg tcgatctgct
     5881 cggtcttcga tgcctcgtac tgtttggtcc agcgaccgat ctgccgctcg tagtagttgc
     5941 cggggcggcc gaagtcggcg agccccaccg ctgcggggtc cacgctgtgg agctttgcca
     6001 gaactcgggc gaggtctaag tagatggcgc gccgttcggg cggggtcaga gacggaagca
     6061 gcgtctctgc gaacacgcgt ccgttcacca tctccatcac gtagaacttc gtgccgatca
     6121 cgcctgcgtc ctcgcacagg cacttcatca ggggaacggg cacttcggtc tgctccagcg
     6181 ctttcatcac ccggtactcg cggtccacct ggtgggccga cggcagcagc tcacccgggg
     6241 gctgcttgcg cagaacgtag agcctgccac ccgcttcgag ctgaaaggtc gggttcgact
     6301 gtccgccctc gaactgacgc acc
//
LOCUS       DBNHQR010000200         2269 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1096511, whole genome shotgun sequence.
ACCESSION   DBNHQR010000200 DBNHQR010000000
VERSION     DBNHQR010000200.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1096511"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1386
                     /locus_tag="AC16VR_04565"
     CDS             <1..1386
                     /locus_tag="AC16VR_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017520416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR6010462.1"
                     /translation="RDESDHENPTRLVLVPRSNRVDIERLMSHLFATTDLEKSYRVNL
                     NVIGLDHKPRVKNLAQMLKEWLDYRQETVRRRLSFRLQKITDRLHILDGLLVAYLNID
                     EVIRIIREEDDPKASLMQKFHLSDVQATAILDIRLRQLAKLEEQKLRGEKDELEAEKA
                     DIEKTLKSNARLKTLIKKELLADAEKYGDERRSPLVEAQEASAFSEEDLVSNEPVTVV
                     LSEKGWVRAAKGHDLNVMDLNYRSGDGYGHSAHGRTNEVLTFLDSTGRSYAVAPHSLP
                     SARGQGEPLTGRLKPPAGARFVGVAMGPSGTRVLLASNAGYGFVCTIDDMVTKNKAGK
                     ATLSVPKDGSALPPVALTEEGGLLVATVTNQGRMLVFPLEELPQLARGKGNKLINIPT
                     SAFKSGEEQMIAFAVFAASDDLLVMAGQRHLRMKSKDLEHYVGERARRGRKLPRGFQK
                     VDSLAVETSSG"
     gene            complement(1344..>2269)
                     /locus_tag="AC16VR_04570"
     CDS             complement(1344..>2269)
                     /locus_tag="AC16VR_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010463.1"
                     /translation="PEVAGTSIHGIDNEPLALSGDVRRGRSFPAQIVHNDQSIGVVRV
                     VIEPGAFASAPPAMIVTLSILWMIAVPGVLLLAARLNHVPRLFALRRAVLRQPEEPPA
                     AIIPEPLEPETWFLICINLFNQLSLTRDQSTRELALTRSIAARVAALYGGSVHDLPGT
                     GLLLGFGPDESDERPFHVLCAAFMLSRLLADAESFGRYRLALHTLELAPETALKPSME
                     PVRDTAVLSALAKDNSIVVSNAFYDRLPMSQRLISEPLRHPLLEDLDSTGGGARLAQA
                     LAEPHDDLIEERLAELDDQPDDVSTARESTF"
BASE COUNT          437 a          701 c          779 g          352 t
ORIGIN      
        1 cgggacgagt cagaccacga aaacccgacg cggctggtgc tggtgccgcg ttcgaaccgg
       61 gtggacatcg agcgactcat gagccacctc ttcgccacga ccgatctgga gaagagctac
      121 cgggtgaacc tgaacgtcat cggtctcgac cacaagcctc gggtcaagaa cctggcgcag
      181 atgctcaagg aatggctcga ctaccggcag gagaccgtgc gccggaggct gtcgttccgt
      241 ctgcagaaga tcaccgaccg actgcacatt ctcgacggcc tgctcgtcgc ctatctgaac
      301 atcgacgagg tgatccggat catccgcgag gaggatgatc cgaaggcgtc gctgatgcag
      361 aagttccatc tctccgatgt gcaggcgacg gccattctgg acatccggct gcgccagctc
      421 gccaagctgg aggagcagaa actcaggggc gagaaggatg agctggaggc ggagaaggcg
      481 gacatcgaaa agaccctgaa atccaacgcg cgcctgaaga cgctgatcaa gaaggagctg
      541 ctcgcggatg ccgagaagta cggcgacgaa cggcggtcgc ccctggtgga agcgcaggag
      601 gcaagcgcgt tcagcgagga ggacctggtc agcaacgagc cggtgacggt ggtgctctcc
      661 gagaagggct gggtacgcgc ggcaaagggt cacgatctca acgtcatgga cctgaactat
      721 cggagcggcg acggctacgg tcactccgca catgggcgca ccaacgaggt gctcacgttt
      781 ctcgactcca ccggccgcag ctatgccgtg gcgccccact ccctgccgtc ggcgcgcggt
      841 cagggagagc cgctgaccgg gcggctgaaa ccgccggcgg gcgcccgctt cgttggcgtg
      901 gccatggggc cgtccggtac gcgggtgctg ctggccagca acgccggcta cggcttcgtg
      961 tgcacgatcg atgacatggt caccaagaac aaggccggca aggcaacgct cagcgtgccc
     1021 aaggatggca gcgctctgcc gccggtagcg ctaacggaag aaggtggcct gctggtcgcc
     1081 acggtcacca accagggccg catgctcgtg ttcccactcg aagagctgcc gcagctggcc
     1141 cgcggcaagg gcaacaagct gatcaacatt cccacgtcgg cgttcaagtc tggcgaggag
     1201 cagatgatcg cgttcgccgt tttcgcggcg agtgacgacc tgctggtcat ggccggtcag
     1261 cgccatctcc ggatgaagtc aaaggatctg gagcactacg tgggggaacg ggcccgtcgg
     1321 gggcggaagc tgccccgcgg atttcagaag gtcgattccc tggcggtgga aacgtcatcc
     1381 ggctgatcgt cgagttcagc cagccgctct tcgatcaggt cgtcgtgcgg ttcagcgagc
     1441 gcctgggcca gccgcgcgcc gcctccggtg gagtccagat cctccagcag cggatgccgt
     1501 aagggctcgc tgatgaggcg ctggctcatc ggcaggcgat cgtagaaagc gttgctgacg
     1561 acgatgctgt tgtcctttgc cagcgccgag agcacggccg tatcgcgaac cggctccatg
     1621 ctgggtttca gcgcggtttc cggggccagt tccagcgtgt gcagcgccag tcgatagcga
     1681 ccgaacgact cggcatcggc gagcagcctc gacaacatga aggctgcgca cagcacgtga
     1741 aacgggcgct cgtcgctctc atccgggccg aacccgagca gcagaccggt gcccgggaga
     1801 tcgtgcaccg agccaccgta gagggccgcg acccgcgcgg cgatgctgcg cgtcagcgcg
     1861 agctcgcgcg tggactgatc ccgcgtgagg ctcagctggt tgaacaggtt gatgcagatc
     1921 aggaaccagg tttcgggctc gagcggctcc ggaatgatcg ccgcgggcgg ctcttccggc
     1981 tgccggagca cggccctgcg aagcgcgaac agccggggaa catgattcag cctggcggca
     2041 agtagcagaa ctccgggcac ggcgatcatc cacagaatgc tcagcgtgac gatcatcgcc
     2101 ggcggggcgg aggcgaacgc cccgggctcg atgaccaccc tgaccacacc gatgctctgg
     2161 tcgttgtgca cgatctgcgc gggaaaggag cgcccgcgtc gaacgtcgcc ggaaagggcg
     2221 agaggctcgt tgtcgatgcc gtggatgctc gttccggcta cttcgggca
//
LOCUS       DBNHQR010000201         2292 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_273192, whole genome shotgun sequence.
ACCESSION   DBNHQR010000201 DBNHQR010000000
VERSION     DBNHQR010000201.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_273192"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1686
                     /locus_tag="AC16VR_04575"
     CDS             <1..1686
                     /locus_tag="AC16VR_04575"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHA/PHB synthase family protein"
                     /protein_id="HIR6010464.1"
                     /translation="PGAGSDAFSDEIADRTEGIAAASAFGVDTRAIMDAWLSIVNEAT
                     THPRAVLRAATKLGRELGSIVMGSSELAPAPEDKRFSDAAWEANPIFRRIGQSYVAWT
                     RSLDGWLDESGLEGIERERARFVLDVFKDVLAPVNSLAGNPEALKKLRASDGGSLTRG
                     LKNFLDDLRHNHGYPAVADRHAFKVGKDVACTRGAVVYRNELFELIQYRPTTPEVSPV
                     PMLYVFSQVNRFYLGDLTPDRSLFKRLLDAGIPVFAISWRNPTVEERDWNLDTYADGV
                     ITAITVMQQITKQPKIHLMGLCAGGLVAAAAAGVLAARREDWISTLSLFVNVLDYRPG
                     DSEFGLFVSERSVDAQKAVVRGRGVFAERDVFEMFALLRMEENVMSFFRSNYLMGEEP
                     LKHPLLFWSMDYTRVPAEMQCDFLNLAQYNKLAKKEMRILGKRVDLTRIDYPVYVMAG
                     TTDHITPWKACFRSTQLFGGDVAFVLTNQNHTQTISSREDNRHLKHWVVDNPSDNPDR
                     AMRLATEHEGSWTRHWIGWLDDHSGERVKAPRRMGSGKNPILGEAPGLYVLES"
     gene            complement(1691..2068)
                     /locus_tag="AC16VR_04580"
     CDS             complement(1691..2068)
                     /locus_tag="AC16VR_04580"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6010465.1"
                     /translation="MAQTLASEHGAEMHVAHVIKPLQQAYAGVNPSTMSDVGIQFEHE
                     ADAQARDELARIADVHGLAADRLHVRHGAPAAEIHALVKELRADLVVIGTHGKHGVQL
                     LLGSTANATLHGATCDTLAVRIR"
     gene            complement(2165..2292)
                     /locus_tag="AC16VR_04585"
                     /pseudo
     CDS             complement(2165..2292)
                     /locus_tag="AC16VR_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456271.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
BASE COUNT          419 a          713 c          746 g          414 t
ORIGIN      
        1 ccgggtgccg ggagcgatgc gttctccgac gagatcgccg atcggacgga agggattgcg
       61 gcggcaagcg cgttcggcgt ggataccagg gccatcatgg atgcctggct gagcatcgtg
      121 aatgaagcca ccacgcaccc ccgcgccgtt ctacgtgccg caaccaagct aggtcgcgag
      181 ctgggcagca tcgtgatggg atccagcgag ctggcgccgg cgccggaaga caagcgtttc
      241 agtgatgcgg cttgggaagc caaccccatt ttccgaagga tcggacagtc ctacgttgca
      301 tggacccgat ccctggacgg ttggctcgat gagtcgggac tcgagggcat cgaacgcgag
      361 cgtgcgcgat tcgttctgga cgtcttcaag gacgtgctgg ctccagtcaa cagcctggcc
      421 ggaaaccccg aggcgctgaa aaagctgcgc gccagtgatg ggggtagcct cactcgcggg
      481 ctgaaaaact ttctggacga tctccggcac aaccacggct accccgcggt agccgaccgg
      541 cacgccttca aggtcggcaa ggatgtggcc tgcaccaggg gcgcggtggt gtatcggaac
      601 gagctcttcg agctgatcca gtatcggcca accacccctg aggtctcgcc ggtgcctatg
      661 ctgtacgtgt tcagccaggt gaaccgcttc tacctgggtg atctgactcc tgatcgcagc
      721 ctcttcaagc ggctgctcga tgccggcatt ccggtattcg ccatcagctg gcgcaacccc
      781 accgtagagg agcgggactg gaacctcgat acctatgctg acggcgtgat cacggccatc
      841 accgtcatgc agcagatcac gaagcagccc aagatccacc tcatgggtct gtgtgccgga
      901 ggattggtgg ccgcagcggc ggccggggta ttggcggcgc gccgcgagga ctggatcagc
      961 accctttccc tgttcgtcaa cgtgctcgat taccggccgg gcgacagcga gttcgggctg
     1021 ttcgtcagcg agcgttcggt ggacgctcag aaagccgtcg tccgcgggcg cggcgtcttc
     1081 gcggagcggg acgtgttcga gatgtttgcc ctgctgcgca tggaagagaa cgtgatgagc
     1141 ttcttccgca gcaactatct catgggggag gagcccttaa agcatccgct gctgttctgg
     1201 tccatggact acacccgcgt gccggcggag atgcagtgcg attttctgaa cctggcccag
     1261 tacaacaagc tcgccaagaa ggagatgcgc atcctcggca agcgggtcga cctcaccagg
     1321 atcgactatc cggtctacgt catggccgga accacggacc acatcacgcc ctggaaagcc
     1381 tgtttccgga gcacccagct gtttggtggc gacgtggcgt tcgtcctgac caaccagaac
     1441 cacacccaga cgatctccag ccgggaggac aaccgacacc tcaagcactg ggtcgtcgac
     1501 aacccgtcgg ataatccgga tcgggccatg cggcttgcca ccgagcacga ggggtcatgg
     1561 acgagacact ggatcggctg gctcgacgat cacagcgggg aaagggtcaa agcgccacgg
     1621 cgcatgggct cgggcaagaa ccccattttg ggggaggccc ccggtctgta cgtgctggag
     1681 tcctgaagcc tcagcggatc ctcacggcca gggtgtcgca ggttgcgccg tgaagggtcg
     1741 cgttggcggt ggacccaagc agcagttgca cgccgtgctt gccgtgggtt ccgatcacca
     1801 ccagatccgc cctaagttct ttgacgagag cgtggatctc cgcagccggg gcgccgtgcc
     1861 gcacgtgcag ccggtcggcc gccagcccgt ggacatcggc aattctcgcg agctcgtccc
     1921 gggcctgggc gtcggcttcg tgctcgaact gaattcccac gtcggacatc gtcgatgggt
     1981 tgaccccggc gtaggcctgc tgcagcggct tgatcacatg ggccacgtgc atctccgcac
     2041 cgtgttctga cgccagtgtc tgcgccatgt cgagaacctg gcttgactcg tcggtcagat
     2101 cgacgcaggc gatcaatcga cggtaggcgt gggaaaactc ctcggcttcc tcgccgcgtc
     2161 cgggaattcg gacggccaga acgtgctggt tggtgccatg cagcacggcg ttggcggtgg
     2221 atcccagaag cagcttgagt ccgtgccggc cgtgacttcc gaccaccagc aggtcatgcc
     2281 cgccgctctc gg
//
LOCUS       DBNHQR010000202         4010 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_210166, whole genome shotgun sequence.
ACCESSION   DBNHQR010000202 DBNHQR010000000
VERSION     DBNHQR010000202.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4010)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_210166"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="AC16VR_04590"
     CDS             complement(<1..649)
                     /locus_tag="AC16VR_04590"
                     /inference="COORDINATES: protein motif:HMM:NF012742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR6010466.1"
                     /translation="MRSQKSSQKVTIVDVAKRAGVSIKTVSRVLNSEPNVRADTREKV
                     LKAAKALHYQPNPSARGLAGNRSFSIGLLYENPQEFGYVKDALNGVIGTCEAEGYSLL
                     MRPCGEEVDSEEVRKFVAQTRVDGAVLTAPVCDEPKVTDLLKELGVPFAQLAPKAPRS
                     EWISVQCNDEEASYAMTDYLISLGHRRVGFIKGHPDHGATEKRLAGYRRCLADHDM"
     gene            complement(714..2201)
                     /locus_tag="AC16VR_04595"
     CDS             complement(714..2201)
                     /locus_tag="AC16VR_04595"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6010467.1"
                     /translation="MSSAEHYATAPEDRISFAHKVVYGFGAFVNNLLAAAIGGMAIVL
                     NLGLGMNPALVGLLGALPRLTDALTDPLMGYISDHTNTRWGRRRPYIFAGAIAAGLCF
                     ALLWQLPPGRSESFYFYYFLFGSLIFYLAYTVFATPWVALGYELTPDYHERTRLMGVQ
                     NFIGQLAYLISPWFLWMMQYEPVFDDMVEGAAGLSVAIAVVVMCVGVLPALLLRERFR
                     DVAHAEIAEKQLARGEPRHPLLTNVKSFFAGFAATLRFKPFVRLCAATFLVFNGFMLV
                     SSFQFYVIIYYVFSGDQVTGAEYAGWSGTLSAVSTFVVIGIVTWLATKIGKRRAFFAA
                     TGISIVGYGLKWFCYDPAVPWLLLVPAPLIAFGLGGLFTLMGSMIADVCDLDELASHE
                     RREGMFGSIFWWVVKLGMALALAGGGFLLNATGFDVALGGAQPASAILMMRAFDVIVP
                     MLSSAAAIWIIAGFPITEERAHQVRARLEERRGALNGDPGTAAAV"
     gene            complement(2244..2873)
                     /gene="eda"
                     /locus_tag="AC16VR_04600"
     CDS             complement(2244..2873)
                     /gene="eda"
                     /locus_tag="AC16VR_04600"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - (R,S)-4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HIR6010468.1"
                     /translation="MDACDLLDGVGIVAVVALEDADAAVPLARVLAGSGVSCVEVTLR
                     TGCAVDAIGRIAREVPEVLVGAGSIRRPEQLLEVRDAGARFAVSPGSSDGLLAASRRL
                     KMPFVPGAVTATEMIGLLEHGYFLQKFFPAEASGGARALRSLAAPLPEVRFFPTGGIT
                     QELAPGYLQMGSVACIGGSWFVPADSLRAGDFSAIGRLAGAAAELTTLS"
     gene            complement(2875..>4010)
                     /locus_tag="AC16VR_04605"
     CDS             complement(2875..>4010)
                     /locus_tag="AC16VR_04605"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase domain-containing
                     protein"
                     /protein_id="HIR6010469.1"
                     /translation="GTANSNQMLLEFMGLQLPGGSFVNPGSELRDLLTAAAVRQVLEL
                     TRLGNRHMPIGRIVDESSVVNAVVGLLASGGSTNHTMHLVAVAAAAGIRLDWSDFADL
                     SRVVPLMARVYPNGEADVNHFHDAGGLGFMIGELLDHGLLHDEVETILGRGLSRFVVC
                     PERADGALRWRPAKKSSADPGVLRGVAEPFDAEGGLRVVQGDVGRAVVKVSAVRAERR
                     RISAPARVFVSQDAFAEAFEAEELEGDMVAVLPWQGPRANGMPELHRLSPYLGVLERR
                     GYRVALLTDGRMSGASGAVLAAIQVTPEAASGGLIGRIRDGDLIHIDADAGAVRVEAD
                     LEGRTGTAADAPDGTHGTGRELFAAFRAQVGPAESGASIFGPL"
BASE COUNT          770 a         1373 c         1286 g          581 t
ORIGIN      
        1 gcatgtcgtg atccgcgagg cagcgcctgt aacccgccag ccgcttctcc gtcgcgccgt
       61 gatcgggatg ccccttgatg aaaccgacgc ggcggtgacc gagagaaatc aggtaatcgg
      121 tcatggcgta gctggcttcc tcgtcgttgc actgcaccga gatccactcg gatcggggcg
      181 ctttcggggc gagctgagca aacggcacgc cgagctcctt cagcagatcg gtgaccttgg
      241 gctcgtcgca cacgggtgcc gtcaacacgg cgccgtcaac gcgcgtctgc gccacgaact
      301 tgcgaacctc ctccgaatcc acctcctcgc cgcaaggccg catgaggagc gagtaaccct
      361 ccgcctcgca ggtaccgatg acgccgttca gagcgtcctt gacgtaaccg aattcctggg
      421 gattttcgta aagcaaaccg atcgagaacg aacggttgcc ggcaagcccg cgcgccgagg
      481 ggttcggctg gtaatgcagg gccttggcgg ccttgagcac cttttcccgg gtgtccgcac
      541 gcacgttggg ctcgctgttg agcacccggg acacggtctt gatggacacg cccgcccgct
      601 tggccacatc gacgatcgtg accttctggc tgctcttctg cgatctcatg cgtactccgt
      661 ttccaggctc agcggtaaaa acccatcagc acactttcga aacgccgctc attctatacg
      721 gccgcagccg tccctggatc accgttcagg gcaccccggc gttcctcgag gcgcgctcgt
      781 acctgatgtg cacgttcttc cgtaatcggg aagccggcga taatccagat agccgccgcg
      841 gagctcagca tgggtacgat cacgtcgaac gcgcgcatca tcaggatcgc gctggccggc
      901 tgagcgccgc cgagcgccac gtcgaagccg gtggcgttca agagaaaccc gccgccggcg
      961 agcgccagcg ccatgccgag cttcacgacc caccagaaaa tcgacccgaa catgccttcg
     1021 cgccgttcat ggcttgcgag ctcgtccagg tcgcagacgt cggcgatcat ggagcccatc
     1081 agggtgaaca gaccgcccag gccgaacgcg atcagcggcg ccggcacgag cagcagccac
     1141 ggcaccgccg ggtcgtagca gaaccacttc agcccgtacc ccacaatgga gatccccgtg
     1201 gcggcaaaga aagcccgccg cttgccgatc ttggtggcaa gccaggtgac gatgccgatg
     1261 acgacgaacg tggagacggc cgacagcgtg ccggaccagc ccgcgtactc cgcgccggtc
     1321 acctgatcac cggaaaagac gtagtagatg atcacgtaga actggaagga ggagaccagc
     1381 atgaagccgt tgaagacgag aaacgtggcg gcgcacagcc tgacaaacgg cttgaacctg
     1441 agcgtggcgg cgaagcccgc aaagaaggac ttcacgttgg tcagcaaggg atgccggggt
     1501 tccccacggg ccagctgctt ttccgcgatc tcggcatgag cgacgtcccg aaaccgctcg
     1561 cgaagcagca acgccggcag aacgcccacg cacatcacca ccacggcgat ggctaccgac
     1621 aaccccgcgg cgccctccac catgtcatcg aagaccggct cgtactgcat catccacagg
     1681 aaccagggag agatcagata ggccagctgg ccgatgaagt tctgaacccc catgagccgg
     1741 gtgcgctcgt gatagtcggg cgtcagctcg tatcccagcg cgacccaggg cgtggcgaag
     1801 accgtgtagg ccaggtagaa gatcagcgaa ccgaagagga agtagtagaa gtagaagctt
     1861 tcgctgcgac ctggcggcaa ctgccacaga agggcgaagc acaacccggc ggcgatggcg
     1921 cctgcgaaga tgtaaggccg ccggcgcccc caccgcgtgt tggtgtggtc cgagatgtat
     1981 cccatcaggg ggtctgtgag cgcatcggtg agtcgcggca acgctccgag cagccccacc
     2041 agagccgggt tcatgccgag acccaggttc agcacgatgg ccatgccgcc aatggcggct
     2101 gccagcaggt tgttgacgaa ggccccgaac ccgtagacga ccttgtgcgc aaagctgatc
     2161 cggtcttccg gtgcggtggc gtagtgctcg gcggaactca tcggtaggtg cgacctgtgg
     2221 gcttccgacc gattaggccc gctctatgac agcgttgtca attcagccgc ggccccggcc
     2281 aggcggccaa tcgcagagaa gtcgccggcc cgcaggctat cggcaggaac gaaccaggat
     2341 ccaccgatgc aggccaccga gcccatctgc agatacccgg gggccagttc ctgggttatc
     2401 ccgccggtgg gaaagaagcg cacctccggc agcggtgcgg ccagcgagcg aagcgctctg
     2461 gcgccgccgc tcgcctctgc ggggaagaat ttctgcagaa agtaaccgtg ctccaaaagc
     2521 ccgatcatct ccgtcgccgt gacggcgccg ggcacgaagg gcattttcaa ccggcgcgaa
     2581 gccgccagaa gcccgtcgct cgagcccggg ctcacggcaa agcgggcgcc ggcatcgcgc
     2641 acctcgagga gctgctccgg gcggcggatg ctgcccgccc cgaccagcac ctcgggaact
     2701 tcccgggcga tccgcccgat ggcgtccaca gcgcagcccg tgcgcagggt gacctcaacg
     2761 cagctgaccc cggaaccggc cagaactcgc gccagcggca cggcggcatc ggcgtcctcc
     2821 agcgccacca ccgcgacgat cccgacgcca tcgagcagat cgcaagcgtc catctcaaag
     2881 gggcccgaag atgctggcgc cggactcggc cgggccgacc tgggcgcgaa aggcggcgaa
     2941 tagctcacga ccggtgccgt gggttccgtc gggcgcatct gcagccgtcc ccgttcggcc
     3001 ctccagatcc gcctcgacgc gcacggcgcc cgcatcggcg tcgatgtgga tcagatcgcc
     3061 gtcgcgaatc cgaccgatca gccccccgct ggcagcctcc ggcgtcacct gaatggctgc
     3121 gagcacggcg ccggaggcgc cagacatgcg gccgtcggtc agcagagcga cccggtatcc
     3181 ccgacgctcc agcacgccta agtaggggga gagccgatgc agctccggca tgccgttggc
     3241 gcgcggaccc tgccagggca gcacggcgac catgtcgccc tcgagctctt cggcctcgaa
     3301 tgcctcggca aaggcgtcct gggagacgaa cacccgggcg ggggccgaga tgcgccgacg
     3361 ctccgcccgg accgcggata ccttgaccac cgcgcgcccc acatcgccct gaaccacccg
     3421 gagcccccct tcggcgtcga acggctccgc gaccccgcgc agcacgccgg gatcggcgga
     3481 cgattttttc gcgggccgcc acctcaaggc gccgtccgcc cgctcggggc atacgacgaa
     3541 ccggctgagg ccgcggccca ggatggtttc cacctcgtcg tgcagcaggc cgtgatcgag
     3601 cagctcgccg atcatgaagc cgagcccgcc ggcgtcgtgg aagtgattga cgtcggcctc
     3661 cccgttcgga tagacgcgcg ccatcagcgg cacgacgcgg gacagatcgg cgaagtcgga
     3721 ccagtccagc cggatgccgg cggcggcggc aacggccaca aggtgcatgg tgtgattggt
     3781 ggagcctccg gaggccagca ggcccaccac cgcgttgacc accgaagact cgtccacgat
     3841 gcggccgatg ggcatatggc ggttgcccag acgggtgagc tccagcacct gtcggaccgc
     3901 agcagcagtg agcagatcgc gcagttccga gcccgggttg acgaaagatc cacctggaag
     3961 ctgcaggccc atgaactcca ggagcatctg attgctgttg gccgtaccgt
//
LOCUS       DBNHQR010000203         3426 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_83311, whole genome shotgun sequence.
ACCESSION   DBNHQR010000203 DBNHQR010000000
VERSION     DBNHQR010000203.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_83311"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="AC16VR_04610"
     CDS             <1..579
                     /locus_tag="AC16VR_04610"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR6010470.1"
                     /translation="VPRDGWLLVTAAGSALGKSVVRLGRRHGFRTICVVRSSANAATL
                     GALGADAVIETDRHDLIAEVARLTKGQGVGSALDCVGGPLAGDVVRCLGLGGRLVVYG
                     TLADSPIEVPSRDLMMPLAQVTGFYFGNWMAQQSPLKLLGVLRAVRRLAVEGVFQTEV
                     TDTYPLDQAATALAASLEPGRTGKVMLRIGSR"
     gene            complement(605..1000)
                     /locus_tag="AC16VR_04615"
     CDS             complement(605..1000)
                     /locus_tag="AC16VR_04615"
                     /inference="COORDINATES: protein motif:HMM:TIGR04433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UrcA family protein"
                     /protein_id="HIR6010471.1"
                     /translation="MRRNLFRMAMVALVGTAVGVGAVAQEKEGITVTASRAPEVKVVE
                     PERGSIMQTVSLSYGVTYSDLDLNTTEGKQQLKARVRQAAEDACEELDRQYPLAEPKG
                     ARCVGAAVDGAKDQVRDAVASAGKPIKMQ"
     gene            complement(1330..1406)
                     /locus_tag="AC16VR_04620"
     tRNA            complement(1330..1406)
                     /locus_tag="AC16VR_04620"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1370..1372),aa:Arg,seq:tcg)
     gene            complement(1481..1996)
                     /locus_tag="AC16VR_04625"
     CDS             complement(1481..1996)
                     /locus_tag="AC16VR_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010472.1"
                     /translation="MSACQLRFAAMALGMAAAISSPAVAVEVPYQGIAVLCAVEPPGM
                     TDQQGEITHTIAMAMTFRIETDHELINGTERLESNSRLDATGEGYYWGYGTVVPDSGG
                     GTLEGEFFFWSRAMGSITGTYYGTGDLAGVIMTYRLTPTEPPQGNRPCDGAPAMGGYL
                     ISGAVENYVAR"
     gene            complement(2233..2664)
                     /locus_tag="AC16VR_04630"
     CDS             complement(2233..2664)
                     /locus_tag="AC16VR_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010473.1"
                     /translation="MGLLRKIFSSKPDFPAISPQSTAAMRLATVEKELHELAEQVLDP
                     LEVVPAEHTAYVFLGKPPKKFGLAWIHDGKISGLNTLVEKHGLTPQEVGNVVDQLREA
                     YERNQDVDRYTATVKGVDVVVTPSKEFEKEVHEIIDRIVRH"
BASE COUNT          593 a         1166 c         1048 g          619 t
ORIGIN      
        1 gtgccaaggg acggctggct gctggttacg gccgcgggat ccgcgctcgg caagagcgtc
       61 gtgcgcctcg gcaggcgtca cggctttcga acgatctgtg tggtgcgctc cagcgccaac
      121 gccgcgacgc tcggcgcgct gggcgccgac gcggtcatcg aaacggaccg tcacgacctg
      181 atcgcggagg tggcgcgtct cacgaaaggc cagggtgtcg gctccgcgct cgactgcgtg
      241 ggcggcccgc tcgccggcga cgtcgtgcgc tgcctgggtc tcggtggccg gctggtagtc
      301 tatggcacgc ttgccgacag cccgatcgaa gtgccatcgc gcgacctgat gatgcccctg
      361 gcgcaggtca ccggcttcta ctttggcaac tggatggcgc agcaatcgcc actcaagttg
      421 ctcggcgtgt tgcgtgcagt gaggcggctt gccgtcgagg gcgtgttcca aaccgaggtg
      481 acggacacct atccgcttga tcaggccgcc acggcgttgg cggcttccct cgagccggga
      541 cgaaccggca aggtcatgct gcgtatcggc tcgaggtagc tcccggcacg gacccggttt
      601 gagcctactg catcttgatg ggcttgcccg cggaggcgac ggcgtcgcgg acctggtcct
      661 tcgcgccgtc cactgcggct cctacacacc gcgctccttt cggctcggcc aatgggtact
      721 gccggtccag ctcctcgcag gcatcctcag ccgcctgcct gacccgggcc ttgagctgct
      781 gcttgccttc agtcgtgttg agatccaggt cgctataggt cacgccatag gacagtgaaa
      841 ccgtctgcat gatcgagccc cgctcgggct caacgacctt tacctcggga gcccgggacg
      901 cggtgactgt gatcccctcc ttctcctggg ccacagcacc cacgccaacc gctgtgccaa
      961 ctaacgcgac catcgccatc ctgaagagat tccttctcat ggcactcctc ctgttcaatc
     1021 gacgaacccg gaaaccgctc tcggctgcgt ccgagttcac caataggatg gtacgactgt
     1081 catgcgcagg cgcaatcagg agattgtgaa gcactgctac gctcgcgggg ccgcgtaacg
     1141 acttgtgcca gaggtcccct gcagccctgt cgaccgggaa ctgtcccgct gttcttgccc
     1201 gggcgccggg caggcggcat tcgccttcgc gctgcaaccg ggaccattcc ctggggccgc
     1261 ttcactgaca ccaggccggg ccgttgccga caggctcccc tgagcttccc ggaaaagacc
     1321 aatgcgaagt ggcgcgcccg gacggattcg aaccgccgac cactgcgttc gaagcgcagt
     1381 actctatcca gctgagctac gggcgccgcg ccatgcccgg tatgctgcca gtccggtcgt
     1441 actctcacaa tcggcagatg gcgatcttcg gtgctggcgc tcagcgagcg acgtaattct
     1501 ctacggcacc ggaaatgaga tagccgccca tggccggcgc gccatcacac ggccgatttc
     1561 cctgaggagg ctcagttggg gtcaaccggt aggtcatgat gacgccggcg aggtccccgg
     1621 taccgtaata cgtcccggta atcgacccca tcgcccggga ccagaagaag aactctccct
     1681 ccagggttcc gcctccgctg tccgggacaa cggtaccgta accccagtag tacccctctc
     1741 cggtcgcgtc caggcgcgag ttcgactcca gcctctcagt gccattgatc agctcatgat
     1801 ccgtctcgat gcgaaaggtc atcgccatcg cgatcgtatg ggtgatctcg ccttgctggt
     1861 ccgtcatacc gggcggctcc acggcacaca ggacggcgat cccctgatat ggaacttcca
     1921 cagcgaccgc cggtgaactg atggccgccg ccattcccag ggccatggcg gcaaacctga
     1981 gttggcacgc tgacacccgc aggcagccgg tatccattca ggaccccgcc cggcgcggct
     2041 cgaatcgcat cgaatatgca tgacccgggc gcgaccccaa ccggtcgcca tgccggcccc
     2101 cggtgagccg tccacgcaag cgacgccgga ctccccatga cggagccgct gctatctccg
     2161 gctggccgat catcggacaa cccgccaaca cgaacgcatg cctgacggaa tcggagccag
     2221 gccccaacga tttcagtgcc gcacgatcct gtctatgatc tcgtgcactt ccttctcgaa
     2281 ctccttggac ggagtgacta cgacatccac gcccttcacc gtagcggtgt agcggtccac
     2341 atcctggttg cgctcgtatg cctcgcgtag ctggtccacg acgttgccga cttcctgcgg
     2401 cgtcaggccg tgcttctcca ccagcgtatt gaggccgctg atcttgccgt catgtatcca
     2461 cgcgaggccg aatttcttcg gcggcttccc caggaacacg taagcggtgt gctcggcggg
     2521 cacaacctcc agcgggtcaa gcacctgttc ggcgagctcg tgaagttctt tttcgaccgt
     2581 cgccaagcgc atggcggcag tgctctgcgg cgagatggcc ggaaaatccg gcttgctgga
     2641 gaagattttc cgcaatagtc ccatgacttt ctccttctga ttcgacgccc gtcggggtgc
     2701 ctggccaact gtcatcctaa acaatcgatg ccggagctcc aaacgcggca gctgcagaat
     2761 gcggccattc ccgccggagg ctgcaccgta gaccgccgaa acagcggcag gagccgcctc
     2821 cggccggcgt ggcacgaggt aacacacctc cgtcatcaac ggcgttccgg catcccggac
     2881 gatcctcgtg cgccgcgtgt tgcgcgaaat gctgcaccgc gacagcgacg gaatcgggtc
     2941 ttggtgatga acgagagcat cagttgtcgg ttgtcgccac cgcaggaagg ccaccccgcg
     3001 cctgccgcgc gggaaccatg gcttggtact ggactcggtc gatcacttac gcgatgagac
     3061 gtcggcctga cccgaacgaa ttcacagcgg tggcggccac ccgggctgcg gcggcgccac
     3121 gctttggaag gtagcgtcag gagcaagaag cacgattccg gcttattgaa gttgctgtca
     3181 ggtatttgtc actacaggag cgaggtcgtc tgctgagcct cgccggcggg cggtctcatt
     3241 tcatgatcgg ctccggggcg catgcaccca aggtcccgcg cccagaaacc tgggcgcagc
     3301 gtcgcagaca ccaaaaacct ggtaccaggc cctgggggca cgccgattcg cgcgaatacc
     3361 gggccagccc ggagctaccc gagttcccga tgcccgactc ggcgccgcag cgcgaggatc
     3421 gaggcc
//
LOCUS       DBNHQR010000204         3262 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_336837, whole genome shotgun sequence.
ACCESSION   DBNHQR010000204 DBNHQR010000000
VERSION     DBNHQR010000204.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_336837"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..912)
                     /locus_tag="AC16VR_04635"
     CDS             complement(40..912)
                     /locus_tag="AC16VR_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010474.1"
                     /translation="MYKVFAFLKRNPNLTHDEYRAGHVGYHCGQSRRLKNIRGYLVNV
                     WDNQPLAEKIGDLAGEIAFNQPDGFLDYWDGFPQVYFDDREAWVNARTVEPNRATADG
                     LVVDPDWSLGDSPHLFMPVPGEPTQFQAHHVHMEEHVVVAVRRPEFRLTKLIQFFQKR
                     EGLTDAEFADRLLGEYAPLAGSMPGLWGYVANLRDADPEAAMRGFFAPDSWVFGEEGR
                     ALHGRFCSLWRGANELHFDSVEAFAAARRDDRVRDLLLELERELFSSMWHVEVDENLI
                     VIPNRGPAPDFYFR"
     gene            1102..2499
                     /locus_tag="AC16VR_04640"
     CDS             1102..2499
                     /locus_tag="AC16VR_04640"
                     /EC_number="1.13.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF015043.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006720 - isoprenoid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HIR6010475.1"
                     /translation="MAETTRPATEPTAESDYRFLSGNFAPVAEEVTAFDLPVEGELPA
                     ELNGRYLRNGPNPFDDPDPGSYHWFTGAGMVHGIRLEDGRAEWYRNRYVGSEQLAASR
                     GEPDIPGANWNDNPSGPNTNVGGFAGTTWALVEAGGCPVELTYELETVARNDFFGTLP
                     GAFTAHPKVDPQTGELHGMAYAWPQWMDHVQYVAVAPDGRVRRTVDVKLPGMTMMHDF
                     SLTQRYVVIYDQPVTVDFAMVGNTSFPMRWNPDYGNRLGLLPREGDADEIIWIDAPLG
                     YCFHPMNAYDDPDGSVIVDLCVYERMFDAYPRDPFGDSMASLQRWRVNPANRTISVDV
                     IDASANEFPRHRGSLSTLPYRYGYCASPSSDMSAGWPTLKHDLQTGERSVFDHGPGRA
                     AGEPVFVAREGSAAEDDGYLITYVHDLAQSSAEFVVMDAQDFDRGYLARVALPQRVPF
                     GFHGNWVSDRAVSPG"
     gene            complement(2528..2653)
                     /locus_tag="AC16VR_04645"
     CDS             complement(2528..2653)
                     /locus_tag="AC16VR_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010476.1"
                     /translation="MGSAEMNPFTDMLPKPIDIQRLQSLLTRLLRSRDAVKAADP"
     gene            complement(2635..2886)
                     /locus_tag="AC16VR_04650"
     CDS             complement(2635..2886)
                     /locus_tag="AC16VR_04650"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6010477.1"
                     /translation="MIDTGIGVPEAFRDGLFEQFARADATMTRRYGGPGPGLFISQRL
                     ASLLGGRVEYAPGETGSVFRLVLAICRATSFATRWVLQR"
BASE COUNT          575 a         1139 c         1022 g          526 t
ORIGIN      
        1 cgccggacaa atccaaaaac cttgagcatt cgatccccct cagcggaaat aaaaatcggg
       61 ggcgggcccg cgattcggta tgacgatgag gttctcgtcc acctccacgt gccacattga
      121 agagaaaagc tcacgctcga gctcgagcag aaggtctcgc acccgatcgt cgcggcgggc
      181 ggcggcaaag gcttcaacgg agtcgaagtg cagctcgttc gcgccgcgcc acagggaaca
      241 gaaacggccg tgcagtgccc tgccctcctc gccgaacacc cagctgtccg gagcgaagaa
      301 gccgcgcatc gcagcctcgg ggtccgcatc gcgcaggttc gccacgtagc cccacaagcc
      361 cggcatgctg ccggccaggg gcgcatactc gccgagaagc ctgtcggcaa actcggcatc
      421 ggtgagccct tcgcgtttct gaaagaactg gatcagcttg gtgagccgga actccggccg
      481 ccgcacagca accacgacgt gctcctccat gtgcacgtga tgggcctgga actgcgtggg
      541 ctcgcccggc acgggcatga acagatgcgg gctgtcgccc aggctccagt ccgggtccac
      601 gaccagccca tcggccgtgg ctcggttggg ctccacggtg cgggcgttca cccacgcttc
      661 ccggtcgtcg aagtagacct gggggaaccc gtcccagtaa tcgagaaaac cgtccggctg
      721 gttgaaggcg atctccccgg ccagatcacc gatcttctcc gccaggggct ggttgtccca
      781 cacgttcacc aggtagccgc ggatgttctt cagccgccgc gactgaccgc agtgatagcc
      841 cacgtgcccg gcgcggtact cgtcgtgagt caggttggga ttgcgtttca agaacgcgaa
      901 gactttgtac atggatccgg cagctccccg gcaaccgctc gctcaacccg gagcatacac
      961 gaagctcagc cggcttcatt gccaacgggg caggagaagc gccggcaccg tgtcccgcgg
     1021 gccggcggga cgcgctatga ttcggcctga cgccgcaccg gtcttccaac gtccgcggcc
     1081 ccaccatcgc aggaacaccc catggcagaa acgacccggc ccgcaacgga acccacagcc
     1141 gaaagcgact atcgatttct cagcgggaac ttcgccccgg tggccgagga ggtcaccgcg
     1201 ttcgatctcc ctgtggaggg ggagctgccg gccgagctca acggccgcta cctgcgcaac
     1261 ggcccgaacc cgttcgatga ccccgatccg ggcagctatc actggttcac cggcgcgggc
     1321 atggtgcacg gcattcgcct tgaggacggc cgcgcggagt ggtaccgcaa ccgctatgtg
     1381 ggctctgagc agcttgccgc gtctcgcggc gagcccgaca tccccggagc gaactggaac
     1441 gacaacccct ccgggcccaa caccaacgtc ggcggcttcg cgggtaccac ctgggcgctg
     1501 gtcgaggccg gcggctgccc ggtggagctc acctacgagc tggaaaccgt ggcgcgcaac
     1561 gactttttcg gtaccctgcc cggcgcgttc acggcccacc cgaaggtaga cccccagacc
     1621 ggcgagctgc acggcatggc ctacgcctgg ccccagtgga tggaccacgt gcagtacgtc
     1681 gccgtggcgc ccgacgggcg ggtccgccgc acggtggacg ttaagctgcc cggcatgacg
     1741 atgatgcacg acttctcgct cacccagcgt tacgtcgtca tctacgacca gcccgtgacc
     1801 gtggacttcg ccatggtggg caacacgagc ttccccatgc gctggaatcc ggactacggt
     1861 aaccgcctgg gcctgctgcc gagagaaggc gacgccgacg agatcatctg gatcgacgcg
     1921 ccgctcggct actgcttcca cccgatgaac gcctacgatg accccgacgg ctcggtgatc
     1981 gtcgacctct gcgtctacga gcgtatgttc gacgcgtacc ctcgcgaccc cttcggcgac
     2041 agcatggcct ctcttcagcg ctggcgcgtg aatcccgcca accggacgat cagcgtcgac
     2101 gtgatagacg cctcagccaa cgagtttccg cgccatcgcg gcagcctttc gaccctgccc
     2161 tatcgctacg gctactgcgc atcgccgtct tccgacatgt ccgccggctg gccgacgctc
     2221 aagcacgatc tgcaaacggg cgagcgcagc gtcttcgatc acgggcccgg ccgggcggcc
     2281 ggcgagcccg tgttcgtcgc ccgggaaggc tcggcggcag aggacgacgg ctacctgatc
     2341 acctacgtcc acgatctcgc acaaagctcg gcggagttcg tggtgatgga cgcccaggac
     2401 ttcgatcgcg gctatctggc ccgggtggcg ctgccgcagc gggtgccctt cggctttcac
     2461 ggcaactggg tgagcgatcg cgccgtatcg cccggttgac ggcagctcct ggggatttcg
     2521 agcccggtca gggatcggca gctttgacgg cgtcgcgaga tctcagcaat cgcgtcagca
     2581 gcgactgcaa tcgctgaatg tcgatgggct tcgggagcat gtccgtgaac ggattcatct
     2641 ctgcagaacc catcttgtgg cgaaggacgt cgccctgcag atcgccagca ccagacggaa
     2701 aacactgccg gtctcccccg gcgcgtattc gaccctgcct cccagcagcg aagccagccg
     2761 ctggctgatg aaaagccccg gaccggggcc accgtagcgt ctggtcatcg tggcgtcagc
     2821 ccgcgcaaac tgctcaaaga ggccgtctct gaatgcctct gggacgccta tccccgtatc
     2881 gatcacggtg atcgctttct atcctcgaca gatccagcac gtcctgaatg agattcccga
     2941 gatggcgggc cccgagcacg atggcggtga ggctctcgcg tttcgggtcg tcctcagcgc
     3001 aacatgcgcc tcgatccacc cggccgccat ggccatcagg tcgagcgagc cgctatgctc
     3061 gcggcgtcga cgcaagaccc gcaggcgcct gagcccacat gccgaccgtc accgacattt
     3121 ccacccgcga cgcccgccgc ctgttcctgc accggcaggg actgctgcgg cgcgagccgt
     3181 tcggccgcgg gggtcaggcc gtggcccgcg ccgtcgagcg cctgggctgg gtgcagatcg
     3241 acaccatctc ggtggtggag cg
//
LOCUS       DBNHQR010000205         3910 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_716769, whole genome shotgun sequence.
ACCESSION   DBNHQR010000205 DBNHQR010000000
VERSION     DBNHQR010000205.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3910)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_716769"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="AC16VR_04655"
     CDS             <1..368
                     /locus_tag="AC16VR_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010478.1"
                     /translation="GVDLTVSDESGTTLEVAALDPDPLGLEDVQYTGTMNLAHTPQGL
                     RLVQVESGHSATRVERLSARILHFDGASWGNERLAPYRVIAASVALEDIVLPPVRFVC
                     KPDEPAFTGTEPVGEPSGK"
     gene            complement(382..861)
                     /locus_tag="AC16VR_04660"
     CDS             complement(382..861)
                     /locus_tag="AC16VR_04660"
                     /inference="COORDINATES: protein motif:HMM:NF018049.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated ZapG family protein"
                     /protein_id="HIR6010479.1"
                     /translation="MYSLAWLVVVGVTAAVAGVAMGYLVAQVRSRSRERIRDLEAELS
                     KAQDELAGYRREVFEQFAETARKFKTLNESYSDLHRQLASSASLLCGEAAGPLLAAPP
                     RGAEITAQAEPSPEAEVEPPIVVAEVEDPATGADPQPGDEDSLAAQDGAVPRAREAG"
     gene            complement(956..1429)
                     /locus_tag="AC16VR_04665"
     CDS             complement(956..1429)
                     /locus_tag="AC16VR_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020799109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR6010480.1"
                     /translation="MSNEALQNAYETLSARIGKSSEPTDWFEITQERINSFADATMDH
                     QWIHIDPERAAAGPFGTTIAHGQLTQSIMSFLPGGEGVGLPALDGMQMGINYGWNRVR
                     FPSPVPVGANIRTSAAIKGVEIKGNMLEVVNELTVEVKGQDKPACVAESVLRLVF"
     gene            1569..2981
                     /locus_tag="AC16VR_04670"
     CDS             1569..2981
                     /locus_tag="AC16VR_04670"
                     /EC_number="1.13.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF015043.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006720 - isoprenoid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HIR6010481.1"
                     /translation="MAKPFPKHPHLEGGFAPIHMECDAPDLVVEGEVPRELNGVFYRN
                     GPNAQFAPRGDYHLFAGDGMIHGFYIRNGQVAYKNRWVRTVKWTREHEAGGALFSAFN
                     PMDSDESVRGMETDGLANTNVVWHGGKLLALEEAHAPFELDPYTLESKGAWRYDGKLE
                     GPMTAHPKIDCETGEMLMFGYNAKGLISEDMAFHVVDGDGNLTRSEWFKAPYASMVHD
                     FMITRDHVIFPIMPLTGSMERAMQGGPVYAWEPDKGVHIGIMPRNGSVDELRWFKGDP
                     SFVFHPMNAHSDGNMVTCDVCEFEEAPLFPAPDGTPGDPDKAVPRLTRWTFDLGSNTD
                     DYSSERLDDLACEFPRLDERFTGLGYRHGYFACDLKPEFQVGAFNGIGHVDHATGKID
                     VYAVGQACATNEPIFVPRTEDSAEGDGFLLANVYDAERKASHLVILDAQNVSAGPLAK
                     AYLDHRVPFGFHGNWMPGQA"
     gene            3011..>3910
                     /locus_tag="AC16VR_04675"
     CDS             3011..>3910
                     /locus_tag="AC16VR_04675"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004130 - cytochrome-c peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HIR6010482.1"
                     /translation="MLRRVLATVFLCTALPLCHAAMTAADLGRLLFFDANLSLQRTQS
                     CASCHDESRAFSDGRDNGVRGAASLGDDGRSLGDRNAPALTYVALTPPFRIRGEGQPA
                     GGFFLDGRVATLQEQAAEPFVNPLEMQMPDPTAVVARVRENPTYERSLRRLFGEAVFE
                     DTDTAYRAVGESLAAFEQADELASFDSRYDRHLRGEYQMSEQEALGRDLFFSQLTNCS
                     LCHALSPHATNDARETFTDYGFRNIGVPANAHLRSANGTKPGHTDRGLLENPAVERAE
                     HAGKFKVPTLRNVAVTAPYMHNGV"
BASE COUNT          710 a         1342 c         1223 g          635 t
ORIGIN      
        1 acggcgtgga tctcaccgtc agcgacgaaa gcggcacgac gctcgaggtc gccgccctgg
       61 accccgatcc tctgggcctg gaagacgttc aatacacggg aaccatgaac ctggcccaca
      121 cgccccaagg cctgcggctc gttcaggtgg agtccgggca ctcggccaca cgggtggagc
      181 gcctgagcgc gcgaatcctc cactttgacg gcgcctcctg gggcaacgaa cgcctggcac
      241 cgtaccgggt gattgccgca tccgtggcgt tggaggacat cgtgctgccg ccggtgcggt
      301 tcgtgtgcaa gccggacgaa ccggcgttca ccggcaccga gccggtaggc gagccgtcag
      361 gaaagtaagc ttgaaaggag gctagccggc ttcgcgcgcg cgcgggaccg ccccatcttg
      421 cgcagcgagt gaatcctcgt cgcctggctg cgggtcagcg cccgtcgcgg ggtcttctac
      481 ttccgcaacg acgatgggcg gttcgacttc cgcttcgggc gacggctcgg cctgggcggt
      541 gatttccgcc ccccgcggcg gcgcggcaag caacggcccg gccgcttccc cgcagagcag
      601 gctggcgctg gaggccagct gacggtgcag atcgctgtag gactcgttca gggtcttgaa
      661 cttgcgcgca gtctccgcga actgctcgaa aacttcccgc cggtagccgg caagctcgtc
      721 ctgagccttg ctcagctccg cttccagatc ccggatcctt tcccgagacc gactgcgcac
      781 ctgcgccacg agataaccca tagcgacgcc ggctacagcc gccgtcacgc ccaccaccac
      841 gagccaagcc agggaataca tcgaccgtta cctcgcacta cgtcgccaga ccgatgggcg
      901 cattctactc ggagttcgag ctcaaaacct caaggggtgc aggggcgcgg ggtactcaga
      961 agaccaggcg cagaacgctc tccgcgacac acgccggctt gtcctgcccc ttcacctcaa
     1021 cggtgagctc gttgacgact tccagcatgt tgcctttgat ctcgacgccc ttgatcgccg
     1081 cgctcgtccg tatattggcc ccaaccggca ccggggacgg aaagcgcacc cgattccagc
     1141 cgtagttgat gcccatctgc atgccgtcaa gcgccggcag ccccacacct tcaccgcccg
     1201 gaaggaagct catgatcgac tgcgtcagct ggccgtgagc gatcgtggtg ccaaacgggc
     1261 ccgccgccgc acgttcgggg tcgatgtgga tccactggtg atccatggtg gcgtcggcga
     1321 acgagttgat gcgttcctgc gtgatctcga accagtcggt aggttctgaa gacttgccga
     1381 ttcgggcact tagcgtctcg taagcattct gcagagcttc gttcgacatg tgatttcccc
     1441 caggttgaaa gagcgccgat tatctacgcc gcgccgaatc atgtcatgcg cgcccgagca
     1501 gagcccggac atcgctcgcg caaagggcgt caaaccctct atagttgagc aaacacagga
     1561 ggtggcccat ggctaaacca ttcccgaagc atcctcacct ggaaggcggc tttgccccca
     1621 ttcacatgga gtgtgatgcc cccgatctgg tcgtcgaggg cgaggtgcca cgcgagctga
     1681 acggcgtctt ctatcgcaac gggccaaacg ctcaattcgc gccgcgcggc gattaccacc
     1741 tctttgccgg agacggcatg atccacggct tctacattcg caatggccag gtggcgtaca
     1801 agaaccggtg ggtgcgtacc gtcaagtgga ccagggaaca cgaagccggc ggggcgctct
     1861 tcagcgcgtt caaccccatg gactccgacg agagcgtacg cggcatggag accgacggcc
     1921 tggcaaacac caacgtggtg tggcatggcg gcaagctgct ggccctcgag gaggcacacg
     1981 ctccgttcga gctggatcct tacaccctgg aatcgaaggg agcctggcgc tacgacggca
     2041 agctggaagg cccgatgacg gcgcacccca agatcgactg cgagaccggt gagatgctca
     2101 tgttcggcta caacgccaag ggcctcatct ccgaggacat ggccttccac gtggtggacg
     2161 gcgacggaaa cctcacccgc tccgagtggt tcaaagcgcc ctacgcgtcc atggttcacg
     2221 acttcatgat cacccgggat cacgtgatct tccccatcat gcccctgacc ggatccatgg
     2281 agcgggccat gcagggggga cccgtctacg cctgggagcc agacaagggc gtccacatcg
     2341 gcatcatgcc gcgcaacggc agcgtggatg agctgcgctg gttcaagggg gatccctcgt
     2401 tcgtctttca ccccatgaac gcgcacagcg acggcaacat ggtgacctgc gacgtctgcg
     2461 agttcgagga ggcccctctc ttcccggcac ccgacggcac cccgggcgac cccgacaagg
     2521 cagtcccccg gctcacccga tggacgttcg acctgggcag caacaccgac gactactcaa
     2581 gcgagcggct cgacgatctc gcctgcgagt tcccgcgact ggatgagcgc ttcaccgggc
     2641 tcggctaccg gcacggctac ttcgcctgcg acctgaagcc cgagttccag gttggcgcgt
     2701 tcaacggtat cggccatgtg gaccacgcca cgggcaagat cgacgtctac gccgtggggc
     2761 aggcctgcgc gaccaacgag ccgatcttcg tgccccgcac cgaggactcg gcggagggtg
     2821 acggctttct gctcgccaac gtctacgacg ctgagcgcaa agccagccac ctcgtgattc
     2881 tcgatgccca gaacgtgagc gccgggccgc tggccaaggc ctacctggat caccgggtac
     2941 ccttcggctt ccacggcaac tggatgccgg gccaggcctg agcacggctc ggcccgaccc
     3001 ggaaggctcg atgctcaggc gcgtgctggc gacggttttc ctgtgcacag cgttgccgct
     3061 ctgccacgcg gcgatgacgg ccgccgatct cggccgactg ctgttcttcg acgccaacct
     3121 ctcactgcag cggacccagt cctgcgccag ctgccatgac gagtcccggg cgttttccga
     3181 cggtcgcgac aacggcgtga gaggcgcggc ctccctggga gacgacgggc ggtctttggg
     3241 cgaccggaac gccccagcac tgacctacgt cgccttgacg ccgccgttcc ggatccgcgg
     3301 cgagggccaa ccagcgggcg gcttcttcct cgacgggcgc gtcgccaccc tgcaggaaca
     3361 ggccgcggag ccgttcgtga acccgctgga gatgcagatg cccgatccca ccgccgtggt
     3421 ggcgcgggtg cgggaaaacc cgacctacga gcgttccttg cggcggctgt tcggagaggc
     3481 ggtattcgag gacaccgaca cggcctaccg cgccgttggc gaaagcctcg ccgccttcga
     3541 gcaggcggac gagctcgcca gcttcgattc ccgctacgac cggcatctgc ggggcgagta
     3601 tcagatgagc gagcaggagg ccctcggccg ggatctgttc ttctctcagc taaccaactg
     3661 cagcctctgc cacgcgctca gcccccacgc caccaacgac gcgcgggaga cgttcacgga
     3721 ctacgggttc cgcaacatcg gcgtgccggc caacgcccac ctgcgcagcg cgaacggcac
     3781 gaaacccgga cacacggacc gcggcctgct cgaaaacccg gccgtcgaac gcgcggagca
     3841 cgccggcaag ttcaaggtgc ccactctacg caatgtggcg gtgacggccc cgtacatgca
     3901 caacggggtg
//
LOCUS       DBNHQR010000206         7564 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_83586, whole genome shotgun sequence.
ACCESSION   DBNHQR010000206 DBNHQR010000000
VERSION     DBNHQR010000206.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7564)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7564)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7564
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_83586"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3152
                     /gene="mfd"
                     /locus_tag="AC16VR_04680"
     CDS             <1..3152
                     /gene="mfd"
                     /locus_tag="AC16VR_04680"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017639776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HIR6010483.1"
                     /translation="REPTLLVVPVQTLLQRVAPRTYLDGHSLSLRTGQHFDLDAQRGR
                     LNAAGYNAAETVSAHGEYAVRGSLMDIYPMGADLPIRIDLFDDEVESLRTFDPDTQRT
                     VEKIDAIRLLPAKEFPLDGNAIARFRSNWHNTFNVDVRRCSVYQDISEGISPNGIEYY
                     LAFFFEELATLFDYLPANTVVIREAGVREAAERFLHDVGVRYESLRHDVERPILPPSD
                     VYLRREELNAALKAWPQIVINDASARHAVDFATQACGDLEANPRLRDPARGLCEFLDG
                     ITQPVLFVADSAGRREVFLEFLNAAGIRPRETADLPAFRDSRLDCAIAVAPVEQGLWT
                     DELVIVTESQVFGHQLEERRARSARVLDPEQIIRNLTELHVGDPVVHIEHGIGRYLGL
                     TTLEIDGAPSEFLAVAYAEDAKLYVPVTSLHLISRYAGAEHAPLHRLGSDAWDKAKRK
                     AAERAHDVAAELLNIYARRASHSSYRFNDPSADYRRFAAQFPFELTPDQGRAIDEVIA
                     DLCSERATDRLICGDVGFGKTEVAMRAAFLAVQSNKQVAVLVPTTLLAQQHFETFRDR
                     FADWPVQIEALSRLRSESEVEAIAERMASGRIDVIIGTHKLLNPALSFNDLGLVIIDE
                     EHRFGVRQKERLKALRAEVDVLTLTATPIPRTLNMAMSGMRDLSIIATPPAKRLSIKT
                     FVQEKRQHVIKEAVTRELMRGGQVFYVHNEVRTIERAAAELAELVPEARIGVGHGQMP
                     KRQLERVMNDFYHRQLNVLVCTTIIETGIDIPNANTIIMERADKLGLAQMHQLRGRVG
                     RSNRQAYAYLLTPHPKAITKDAVKRLEAIEAAGELGVGFTLATHDMEIRGAGELLGEE
                     QSGQIESVGYSLYMDLLDRAVKAIQDGKTPNLNAPLEPIAQEVNLHTPTLIPEHYLPD
                     VHARLILYKRIANAADPTELDDLQAEMIDRFGLLPEPLKSLFAVTALKLKTQPLGVVR
                     VDLGESGGRIEFSPDTEVDPLAIVNLVQSHPGTYRLDGATMLRVNRTMPDFQARITFA
                     GELLDRLAAHTPNRLVANA"
     gene            3149..4093
                     /locus_tag="AC16VR_04685"
     CDS             3149..4093
                     /locus_tag="AC16VR_04685"
                     /inference="COORDINATES: protein motif:HMM:NF022425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsiV family protein"
                     /protein_id="HIR6010484.1"
                     /translation="MSAMSPRRAAATAGLAAALLGQASDAMEAEEPVDLLLKDWYVTE
                     IIVVQRPPVMDFNSAERLLRDRPPALPLAMRTFATDPSQLGKDLDLDLSTRLSLVHPT
                     LALLPPAQDEADVDEGSGAMVAPAIEPVLEPDPLLDVLAMAAALEEQLEARSFQLIPA
                     EELTMSASADRLARRSGFQVLYHGAWLQPVPPRDAPEYLLLQTGARVADRHQLEGVVA
                     VTLGRYLHFQASFSYTEPGMGLEPMTLVLNPDGSGTPARWPVETRPVMIMNQSRRMRS
                     EEVHYLDHPKLGIIVRIDPVPVPEELVAAFDALEEPTE"
     gene            complement(4052..4792)
                     /locus_tag="AC16VR_04690"
     CDS             complement(4052..4792)
                     /locus_tag="AC16VR_04690"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003245249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HIR6010485.1"
                     /translation="MRRLAVLVGSGALKLPFDVLVEDFRDTPYGEASSKPSRAAGRNF
                     IVHVLARHGVPHAVAPHLINYRANLHLLSELGVEEIVALNTVGGIAKHARNGRIVLPD
                     QIIDYTWGRAHSFAAEGDLKHVDFTEPYDAALRAELLAAARGQGLDVHDGGVYGCTQG
                     PRFESPAEIDRMDRDGCTVVGMTGMPEAGLARELGIRYASLCLVVNPAAGRGDAIDLD
                     HIAVIAREGMEDVARLLGGFFQGIERGD"
     gene            complement(4792..5364)
                     /locus_tag="AC16VR_04695"
     CDS             complement(4792..5364)
                     /locus_tag="AC16VR_04695"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF006605.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HIR6010486.1"
                     /translation="MTARVPTEILTAREAAELVVGAQEVQAAIDRTAVRLAVALEAAN
                     PLVLCLLNGGLPFTAELIKRFSFPLELSYLHATRYGQRTSGSQLEWLARPPVSVAGRH
                     VLLVDDIFDAGETLMQVVEWLRREGAVEVTTAVLVDKAVPRSGAARVDYAALCCPDRY
                     LFGCGMDFRGYWRNLPAIYALPRSLEDEAV"
     gene            complement(5361..6428)
                     /locus_tag="AC16VR_04700"
     CDS             complement(5361..6428)
                     /locus_tag="AC16VR_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014577578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR6010487.1"
                     /translation="MLESLRDFLSQNTWVVEVFAIVFATALVRYAVRRAFERLAGRLE
                     QTHNIYDDALFDAARGPVGVGIWVLGVSWAAEVAGAVGGAEIFDYVDEVREVAIIWLL
                     VWFALRFIRFMEVHLADSSYREEPVDETTAAAVGKLLRAAAIITGVLMAMQEFGFSIA
                     GVLAFGGVGGIAVGFAARDLLANFFGALMIFLDRPFSVGDWVRSPDREIEGTVEDIGW
                     RLTRIRTFDQRPLYVPNSIFTNLAVENPSRMRNRRIYETIGVRYEDVGVLRQIVADVE
                     SMLREHPDIDHGRTLMVNFVEFGPSSLDFFVYTFTKTTVWAEFHAIKQDVLFRIADII
                     EEHGAEIAFPTQTLHVVQPES"
     gene            complement(6445..>7564)
                     /locus_tag="AC16VR_04705"
     CDS             complement(6445..>7564)
                     /locus_tag="AC16VR_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010488.1"
                     /translation="FSKSGLDGQSRTQGSVSAQLEYYRAWNEDRSSLTFTPFVRVDGE
                     DDERTHFDVRELFWSRVGPDWDLHLGAKQVFWGVTEFKHLVDVINQTDLVENIDGEDK
                     LGQPMAHLSVVRDWGILDFYLLTGFRERTFPGSDGRLRPPLPVDDDARYESGAGRWRV
                     DGAVRWQRQLGRLTLGIHHFSGTSRAPVLIPETGPAGQAVLRPFYPVIDQTGLDAQAI
                     VGDWAWKLEAVSSSGYEGRYSAFNAGFERSLVGVLGGRGDLGLVVEYMFDDRDERAFD
                     TLFEHDLALGTRWQMNDVADTQALIGVIWDVKTDEYVVKLEGSRRLGDTWTLLVEGRI
                     FGGSERPMPGVPPSTDPNNRAWPLERDDFLQLELTRYF"
BASE COUNT         1435 a         2592 c         2328 g         1209 t
ORIGIN      
        1 aacgggaacc caccctgctc gtcgtgcctg tccagaccct tctccagcgg gtagccccgc
       61 ggacctacct cgacggccac agcctttcac ttcgcacggg ccagcacttc gacctggatg
      121 cgcaacgcgg ccggctcaac gcggcggggt acaacgcggc agagacggta agcgctcacg
      181 gggagtacgc cgtgcgcggt tcgctgatgg acatctaccc catgggagcg gatctgccca
      241 tccgtatcga cctgttcgac gacgaggtgg aaagcctgcg gacgttcgac cccgacaccc
      301 agcgcaccgt cgagaagata gacgcgattc ggctgctgcc cgcaaaggaa tttcccttgg
      361 atggcaacgc catcgcacga ttccggagca actggcacaa cacgttcaac gtcgacgtgc
      421 ggcgatgcag cgtctaccag gacatcagcg agggcatctc cccaaacggc atcgagtact
      481 acctggcctt cttcttcgaa gagttggcta cgctcttcga ctacctgcca gccaacaccg
      541 tggtcatccg ggaggccggc gttcgggaag ccgccgagcg gtttcttcac gacgttggcg
      601 ttcgctacga gagcctgcgt cacgacgtgg agcgacccat tcttcccccg tccgacgtct
      661 acctgcgcag agaggagctc aacgccgcgt tgaaggcctg gccgcagatc gtcatcaacg
      721 acgccagcgc ccgtcacgcg gtcgacttcg caactcaggc ctgcggagat ctggaagcca
      781 atccccggct gcgggacccc gcgcgcggcc tgtgcgagtt tctcgacggc atcacccagc
      841 cggtgctttt cgtggccgac tcggccgggc gccgcgaggt gttcctggaa ttcctgaacg
      901 cggccggcat acgtccgcga gagaccgccg acctgcccgc gttccgagat tcgaggctcg
      961 actgcgcaat cgccgtcgcc cccgtggagc agggtctgtg gaccgacgag ctggtgatcg
     1021 ttaccgagag ccaggttttc gggcatcagc tcgaggagag acgcgcccgg agcgctcgcg
     1081 tgctggaccc ggaacagatc atcaggaacc taaccgagct gcacgttggc gaccccgtgg
     1141 tccacatcga gcatggcatc ggtcgctatc tcggcctgac caccctggag atcgacggcg
     1201 cgccgagcga gtttctcgcc gtggcctacg ccgaagacgc gaagctttac gtgccggtca
     1261 catcgctgca cctgatcagc cgctacgctg gcgcagagca cgcgccgctg catcgcctgg
     1321 gatcggatgc ctgggacaag gccaagcgca aggcagcaga gcgtgcccac gatgtcgccg
     1381 ccgagctcct gaacatctac gcccgccggg ccagccactc cagctaccgc ttcaacgacc
     1441 cgagcgccga ttaccgccgc ttcgcggcac agtttccctt cgagctgacg cccgatcagg
     1501 gccgcgccat cgacgaggtc atcgccgacc tatgctccga gcgggcgacc gaccgcctca
     1561 tctgcggcga cgtcggcttc ggcaagaccg aggtggccat gcgcgccgcc tttctggcgg
     1621 tgcagagcaa caagcaggtg gccgtgctag tgcccacgac gcttctggcc cagcagcatt
     1681 tcgagacctt ccgcgaccgc ttcgccgact ggcctgtgca gatcgaagcg ctgtcacggc
     1741 ttcgctcgga aagcgaggtc gaagccatcg ccgagcgaat ggcgtcgggc cggatcgacg
     1801 tgatcatcgg cacccacaag ctgctcaatc ccgcgcttag tttcaacgat ctgggtctgg
     1861 tgatcatcga cgaggagcac cgcttcggcg tccgtcagaa ggagcggctc aaggctttgc
     1921 gagcagaggt cgacgtattg accctgacgg ccacccccat tccccgcacg ctgaacatgg
     1981 ccatgagcgg catgcgcgat ctctccatca tcgccacgcc gccggccaaa cggctttcaa
     2041 tcaaaacgtt cgtgcaggaa aagcgccagc acgtcatcaa ggaagccgtg acgcgcgagc
     2101 tgatgcgcgg cggccaggtc ttctacgtgc acaacgaagt gcgaaccatc gaaagggcgg
     2161 ccgcagagct ggcggagctg gtccccgagg ccagaattgg cgtcggtcat ggccagatgc
     2221 ccaagcgcca gctcgagcgc gtcatgaacg acttctacca tcgccagctg aacgtgctcg
     2281 tctgcacgac catcatcgag acgggcatcg acatcccgaa tgccaacacc atcatcatgg
     2341 agcgtgccga caagctgggt ctggcgcaga tgcatcagct tcgcggccgg gtcggccgat
     2401 cgaatcggca ggcgtacgcc tacctgctga cgccgcatcc caaggcgatc accaaggacg
     2461 ccgtcaaacg cctggaagcc atcgaggcgg ccggcgagct cggcgtcggt ttcactctgg
     2521 ccacccacga catggaaatc cgaggggcag gcgaactgct tggagaggag cagtcgggac
     2581 agatcgagtc tgtgggatat tccctctaca tggaccttct ggaccgggcc gtaaaagcca
     2641 ttcaggatgg caagacgccg aacctcaatg ccccgctgga acccatcgct caggaggtca
     2701 acctgcacac gccgacgctc atcccggagc attacctgcc cgacgtgcac gcccggctga
     2761 ttctctacaa gcgcatcgcc aacgccgccg acccgacgga gctagacgat ctgcaggcgg
     2821 agatgatcga tcgcttcggg ctcctgcccg agccgctgaa atccctgttt gccgtcacgg
     2881 ccttgaagct gaaaacccag ccgctgggcg tcgttcgcgt ggatctcggc gaaagcggcg
     2941 ggcggatcga gttcagcccc gacaccgagg tcgatccgct ggccatcgtg aacctcgtac
     3001 agagccatcc cgggacgtac cggctggacg gcgcgacgat gctgagggtc aaccggacaa
     3061 tgccggactt tcaggcgcga atcacgttcg caggcgagct tctagatcgc ctggcggcac
     3121 acacaccgaa ccggctggtg gccaacgcat gagcgccatg agcccccgaa gagcggccgc
     3181 cacggcgggt cttgctgccg cgctcctcgg gcaggcatcc gacgccatgg aggccgaaga
     3241 acctgtcgat cttctgctca aagactggta cgtcacggag atcatcgtcg tgcagcgccc
     3301 cccggtcatg gatttcaact ccgccgaacg gctcctgcgg gaccgtccgc cggcgttgcc
     3361 cctggctatg cgcacgttcg ccaccgatcc ctcccagctc ggaaaagacc tggatctgga
     3421 tctgtcgacc cgcctgagcc tcgtgcatcc gaccctggcc ttgctacccc ctgcgcagga
     3481 cgaagcagac gtcgacgagg gatccggggc gatggttgct ccagccatcg aaccggtgct
     3541 cgaacccgac cccctcctcg atgtgctggc catggccgcc gcgctcgagg agcaactcga
     3601 ggcccgcagc tttcagctca tcccggccga ggagctgacc atgtccgcca gcgccgaccg
     3661 gctcgcccgg cgaagcggct tccaggtcct ctatcacggc gcctggctgc aaccggttcc
     3721 gccgcgcgat gcgcccgaat acctgctgct gcagaccggc gccagagtgg ccgaccgcca
     3781 ccagctcgaa ggggtcgtgg ccgtcaccct cgggcggtac ctgcactttc aggccagctt
     3841 ctcttacacc gagcccggca tggggctcga gccgatgact ttggtgctca acccggacgg
     3901 ttcgggcacg ccggcgcgat ggcccgttga aactcgcccg gtgatgatca tgaaccagag
     3961 tcgacgcatg cgcagcgagg aggttcatta cctggatcat ccgaagctgg gcatcatcgt
     4021 gcgcatcgat ccggtgcccg ttccggaaga actagtcgcc gcgttcgatg ccctggaaga
     4081 acccaccgag taggcgcgcc acgtcctcca tgccctcacg cgcaatgacc gcgatgtggt
     4141 ccaggtcgat ggcatctccc cgaccggcag ccgggttcac caccaggcac aagctcgcgt
     4201 aacggattcc cagctcccgg gcgagcccgg cttcgggcat tcccgtcatg ccgaccaccg
     4261 tgcaaccgtc ccggtccatc cgatcgatct cagccggcga ctcgaaacgc gggccctgag
     4321 tgcacccata gacgccgcca tcgtgcacgt ccagcccctg cccccgggca gcagccagca
     4381 gctcggctct cagcgcagca tcatatggct ccgtgaaatc cacgtgcttg aggtcgccct
     4441 ctgcagcgaa gctgtgggcg cggccccacg tgtagtcgat gatctgatcg ggcagcacga
     4501 tgcgcccgtt gcgcgcgtgc ttggcgatcc cgccgacggt attcagcgcg acgatctcct
     4561 cgacgcccag ctcggagagc aggtgcagat tggcccgata attgatcagg tgcggggcga
     4621 ccgcgtgcgg aaccccgtgt cgcgccagca catggacgat gaagtttcga ccggcagcgc
     4681 gcgaaggctt ggaggaggcc tcgccgtagg gggtgtcgcg gaagtcctcg accaacacat
     4741 cgaatggaag cttcagcgca ccggagccca cgagtacagc cagacgtctc atcagaccgc
     4801 ttcatcctcg agggatctcg gcaacgcgta aattgccggt aggtttcgcc agtagccgcg
     4861 aaaatccatg ccgcaaccga agagatagcg gtccgggcag cacagcgccg cgtaatcgac
     4921 gcgggccgct ccgctgcgcg ggaccgcctt gtccactaga acggccgtgg tgacctcaac
     4981 ggccccttct cggcgcagcc actccacgac ctgcatgagg gtctcgcccg catcgaaaat
     5041 atcgtccacc agcagcacgt ggcgtccggc gacgctcact ggggggcggg ctagccattc
     5101 cagctgggac ccgctggtac gctggccgta gcgggtcgcg tgcaggtagg agagctccag
     5161 cggaaagctg aagcgcttga tgagctctgc ggtgaacggg agcccgccgt tgagcaggca
     5221 gagcaccagc gggttggcag cctccagagc aacggcgagg cgcacggcgg tccgatcaat
     5281 cgcggcctgc acctcctgcg ctccgacgac gagctcggcc gcctcccgcg cggtcagaat
     5341 ctcagtgggg acccgagcag tcacgactcg ggctgcacca cgtgcagcgt ctgggtgggg
     5401 aaggcaatct ccgcgccgtg ctcttcgatg atgtcggcga tgcgaaagag cacgtcctgc
     5461 ttgatggcgt gaaactcggc ccagaccgtg gtcttggtga acgtgtagac gaagaagtcg
     5521 agggaggaag gcccgaactc gacgaagttg accatcagcg ttctgccgtg gtcgatgtcc
     5581 ggatgctccc ggagcatgga ttccacgtcg gccacgatct gccgcagcac gccgacgtcc
     5641 tcgtaccgca cgccgatggt ttcgtagatg cgccgattgc gcatccgcga cgggttttcc
     5701 accgccagat tcgtgaatat cgagttcggc acgtagagcg gccgctgatc gaatgtccgg
     5761 atccgggtca gccgccagcc tatgtcctcc accgtgccct cgatctcacg gtcaggggaa
     5821 cgtacccagt caccaacgga gaacggcctg tccaggaaaa tcatgagcgc gccgaaaaag
     5881 ttggccagca ggtcccgcgc ggcgaaaccc accgcgatgc cgccgacacc gccaaaggcg
     5941 aggacgccag caatgctgaa cccgaactcc tgcatggcca tcagaacgcc cgtgatgatg
     6001 gcggcggccc gcaggagctt tcccacggcg gcagcggtcg tttcgtctac cggctcctcc
     6061 cgataagacg agtcggccag atgcacctcc atgaatcgaa tgaaccggag ggcgaaccac
     6121 acgagcagcc agatgatggc gacctcgcgc acctcgtcca cgtagtcgaa aatctcggcc
     6181 ccgcccactg ccccggcaac ctcggcggcc caggaaacac cgagcaccca gatcccgacg
     6241 ccaaccggtc cccgcgcggc atcgaacagg gcgtcgtcgt agatattgtg ggtctgctca
     6301 agacgcccgg cgagccgctc gaatgcccgg cgcaccgcgt agcgcaccag cgcggtggcg
     6361 aaaacgatgg cgaacacctc cacgacccag gtgttctgac tgagaaaatc ccgcaacgat
     6421 tccagcatga ggccctgccg cctgtcagaa gtaccgggtc agctcgagct gaagaaagtc
     6481 gtcccgctcg aggggccagg cacggttatt cggatccgtg gagggcggca cgcccggcat
     6541 cggcctttcg gagcccccga agatgcgccc ctccaccagc agggtccaag tgtcgcccag
     6601 gcggcggcta ccttccagct tgaccacgta ttcgtctgtc ttgacgtccc atatcacgcc
     6661 gatcagcgcc tgggtgtcag ccacgtcgtt catctgccag cgggtgccga gcgccaggtc
     6721 gtgctcgaag agcgtatcga aagctcgctc gtcgcgatcg tcgaacatgt actcgaccac
     6781 cagacccagg tcgccgcgtc ccccgagaac gccgaccaga gagcgctcga agccggcgtt
     6841 gaacgccgag tagcgacctt cgtaaccgct gctggaaacc gcctcgagct tccaggccca
     6901 atctccgacg atggcctgag catccagacc ggtctgatcg atcaccggat agaacgggcg
     6961 cagcacggcc tgcccggccg ggccggtctc gggaatcagc acgggggcgc ggctcgtccc
     7021 cgagaaatga tgaatcccga gggtcaaccg ccccagctga cgctgccagc gaacggcgcc
     7081 gtcgacccgc cagcgcccgg cgccggactc gtagcgggca tcgtcatcca ccggcaaggg
     7141 tggccgcaga cgcccgtcgg agccgggaaa agtccgctcg cgaaagccgg tcagcaggta
     7201 aaagtccagg atgccccagt cgcgcacgac cgagaggtgg gccatgggct gtcccagctt
     7261 gtcttcgccg tcgatgttct ctaccaggtc ggtctggttg atcacgtcga ccaggtgttt
     7321 gaattccgtg acgccccaga aaacctgctt agctcccaga tgaaggtccc agtccggacc
     7381 cacgcggctc cagaacagtt cccgcacgtc gaaatgcgtg cgctcgtcgt cctcgccatc
     7441 gaccctgacg aacggcgtga acgtcaggct ggatcgatcc tcgttccagg cacggtagta
     7501 ctccagctga gcgctgaccg atccctgggt ccgggactgg ccatcgaggc cgctcttcga
     7561 gaaa
//
LOCUS       DBNHQR010000207        10818 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1224041, whole genome shotgun sequence.
ACCESSION   DBNHQR010000207 DBNHQR010000000
VERSION     DBNHQR010000207.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10818)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10818)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10818
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1224041"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="AC16VR_04710"
     CDS             complement(<1..482)
                     /locus_tag="AC16VR_04710"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6010489.1"
                     /translation="MTETYLTDERRMIQEVARDFTRREVLPVANELDPIQGDIPMDLR
                     DKLGEMGYFGIRMAEEHGGLGLGVFEYCLISEELARGWMSVASIIARATSLMNVGGWP
                     LEKREELTQKAARGEYLAAISLSEPDVGSDLAAAKCRAVLDGDEWVITGSKYWCTFAD
                     G"
     gene            complement(486..1397)
                     /locus_tag="AC16VR_04715"
     CDS             complement(486..1397)
                     /locus_tag="AC16VR_04715"
                     /inference="COORDINATES: protein motif:HMM:NF015297.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HIR6010490.1"
                     /translation="MNRQPKRARRCQLSVPGSSEKMMAKAAALDLDHVFLDLEDAVAP
                     SAKPAARGMIVDALNGLHWTPRTRCVRINDVETQWCHDDIIEVVTGAGEKLDTIMLTK
                     AKSAADVMFVHLMLDQLEQKLGLTRRIGIECLIEEVEGMMHVEEIAACSDRLECLVFG
                     MGDYSASQQMQLGHIGSSGGYPPDVWHYPRYKMTIACHANGIDPVDGPYANFRNSEAY
                     GKEAERAFVLGMVGKWAIHPSQIELANAVFTPDPAAIADARKQKAAYNEALSQGLGAI
                     NVDGVMVDAASIRIVQNLLDRAELIGM"
     gene            complement(1394..1897)
                     /locus_tag="AC16VR_04720"
     CDS             complement(1394..1897)
                     /locus_tag="AC16VR_04720"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039588.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR6010491.1"
                     /translation="MALTGERNYFEDFQAGQVFRHARGKTMCEQDNVGITLQVLNTAE
                     GHFNEDVMQRNALALGDGRGGWNSRLQFGGVTIAMVIGLTMQDTGENALRELKLDKIR
                     LKAPVFHGDTLYAYTEVLETAEADREDAGIVTFRHWGTNQDDKVVFEGERTVMLKRKS
                     HYGAIPQ"
     gene            complement(1900..2394)
                     /locus_tag="AC16VR_04725"
     CDS             complement(1900..2394)
                     /locus_tag="AC16VR_04725"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039588.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR6010492.1"
                     /translation="MSETNDFRDRAVLLSKGNRYEDMTEGRVFDHHWGRTITDGDNAA
                     FTTQTLSFCPLYFNEPYARSLGHPGTVVNPLLVFNTVFGLTVEDLSEGGGPFLGVDDC
                     EFLADVYVGDTLTARSTVVTARDSKGQPAFGIVTWHTEGFNQHGEKVLDYHRTNLVRK
                     RKRD"
     gene            complement(2391..3731)
                     /locus_tag="AC16VR_04730"
     CDS             complement(2391..3731)
                     /locus_tag="AC16VR_04730"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR6010493.1"
                     /translation="MTPTRALAERIAGTQYADIPAPARTVAKQALLDFIGVTMAGIDE
                     PLARMLRDQVNEEGGHPQAQLIGTDERASTVQAALVNGSAGHAHDYDDVHTAMTGHPT
                     VPVAPAVLALAERLGRSGEEAIAAFCAGVDTECILGHYAGPGHYAHGWHATGTLGSFG
                     AAAAAARIMGLDEDATSRALGIAGTQAAGLKSQFGTMCKPLHAGHAAATGVQSASLAQ
                     RGFDSRRDILEVEQGFMATQASGACEEGFARAMERDGYTQDICFKYHAACYLTHSSIE
                     AVNNLCRRNAFDPNDIESIEIRVDAGHFRVCNILEPRSGLEAKFSLRFTAAMALAGVD
                     TASIEIFTDALTQQPELVAFRDKVRVVAHETPNPDSIVTITTRDGSTHREAVNVAIPE
                     RDLDAQWEKLEHKFHALVDDRVGRAHADELAGLVRTLDSQDDLGDFFSLLRVHR"
     gene            3897..4745
                     /locus_tag="AC16VR_04735"
     CDS             3897..4745
                     /locus_tag="AC16VR_04735"
                     /inference="COORDINATES: protein motif:HMM:NF018677.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrase ferredoxin"
                     /protein_id="HIR6010494.1"
                     /translation="MPERVYCSEFNGDEPMLGTADVVDVWLMLEYRPAWKARALEESA
                     LAAPTRRWLAAGIDALAAAGLRARPQLVRQPELDRDTVSLLVAFGDRLLEFQGTGYDF
                     LNGVDLAGLARGHLPEGARVVSEPRYFVCTNGQRDLCCARFGLPVYAAMRERLGSRVW
                     QVSHLGGHRFAPNVLVLPQATLYGRVQPADVPAFIERVESGEVSLAHLRGRTEYAKPV
                     QAAAGYCGGGELDLVDARGDDDAATVTFRRGSEEITVAVERSDPVDVLKSCGKASEPA
                     YPYRRR"
     gene            complement(4693..5553)
                     /locus_tag="AC16VR_04740"
     CDS             complement(4693..5553)
                     /locus_tag="AC16VR_04740"
                     /inference="COORDINATES: protein motif:HMM:NF039989.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR6010495.1"
                     /translation="MPHIVDTLIEERARGLMQHPLLWRLVTGLLYPVLGYRAAVRMAD
                     TVADMGGLEIFEMLSAQLKLDIDLGGTEHIPETGSAVVTANHPTGIADGVAVFDVLRT
                     VRSDITFFANRDAIRFAPGLADVIVPVEWVEDKRTHARNRETVSHMVRAFRSERLIVI
                     FPSGRLARPSLRGLVERGWTPTAVNLAQKYRCPIVPMHIRGRNSWLYYLLYAINTELK
                     DMTLFRELLNKTRQPYRIRIAEPFDPVGDARMLTLALREFIADEMPGGERRFSVSDTD
                     TLAPTPFRSS"
     gene            complement(5541..6317)
                     /locus_tag="AC16VR_04745"
     CDS             complement(5541..6317)
                     /locus_tag="AC16VR_04745"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6010496.1"
                     /translation="MHLDWQIHDGKGPHLLLVHGFLCSRAQWLWNLTVLAEVCRPVTV
                     ELWGHGRSPAPSDPECYAPDRIVEEFESIRTALGAEQWFVLGYSLGAGLTIRYALTHP
                     HRVMGHVFTNSTSGLADAEQIRRWQDEADETAQRIIAAGQAALERIPVHPRHATKLPD
                     PLHQALMADAARLDPTGVANVLRHTNPNVSVRADVHRNTRPALLVCGSRERRFQPHRA
                     FAQATMPCLEIVDIPAGHGVNMEAHEPFNAAVSSFVRCHT"
     gene            6433..8124
                     /locus_tag="AC16VR_04750"
     CDS             6433..8124
                     /locus_tag="AC16VR_04750"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl-D-amino-acid deacylase family protein"
                     /protein_id="HIR6010497.1"
                     /translation="MAYDILIRNGTVVDGSGGAPYPADVGIRNGRIASIGRIREKART
                     TIDAEGHVVSPGFVDGHTHMDAQVFWDPIGSSSCYHGVTSVVMGNCGFTLAPCRRDEA
                     DLVFRNLERAEDLSRDAMLTGIDWQWETFPQFLDAVDGLPKGINYAGYIGHSALRTYV
                     MGERAFTDPATDGDLAAMCRQVQEAVRAGAIGFSTSRTFNHITADDRPVASRLAEWDE
                     VRAIVNAMGETGKGIFEIAGEAPGRDPARIRDYHERLRDLAVESGVTQTWGMFSVRIA
                     PDFWRPYFDLLDETAAAGGRMYAQVHSRALNVLLSFKTSTPFDKWDVWNDVRSLPLDE
                     QKAKLRDPALRARLVEAANREYQGPRVVGAEARPPNWEWVYPMADMSYDQPSMAELAA
                     ERGVDPVELMIDMSLAHDFDIFFRQPIANENQDDVLEMMKHPRAMVTFSDSGAHVSQI
                     MDSSLQTHLLSYWVREKQAFTLPEAVRRITYDTATMWGLHDRGLVRAGMAADLVIFDP
                     ETIGPRMPTVEHDLPAGARRLKQTADGIMHTIVNGEVLLTRNEYGGATPGRLLRA"
     gene            complement(8172..8248)
                     /locus_tag="AC16VR_04755"
     tRNA            complement(8172..8248)
                     /locus_tag="AC16VR_04755"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8212..8214),aa:Arg,seq:tcg)
     gene            8355..9566
                     /locus_tag="AC16VR_04760"
     CDS             8355..9566
                     /locus_tag="AC16VR_04760"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6010498.1"
                     /translation="MLVGCAMNELPLVGVTVLDFGQVYNGPYCGFLLAQAGARVIKVE
                     SPRGETLRSRGETTGASYPFAMLNGNKECISLNFRSARGQALLRDLVMHVDVVLENFA
                     PGTLERYGVGARTLRGLNPELIYASSTGYGRSGAHRDYRGMDVTIQAMTGVMSITGEG
                     DGPPLKAGPALADFLGGVHLYGGIVTALYRRSQTGEGAVIDISMQDCVFPSLTTALGA
                     YFVAGGQQGPRTGNRHQGLSVAPYNVYAARDGYVAIICIREGHWRGLTRAMGRPELAE
                     DAQFADMRARAQNMDAVDALVEGWTRTLDREEIFARAQAEGVICAPVQTLEDVINDPH
                     LHERGSLHWHEHPRLGPIVLFNSPLRFEGVDLPEVAEVPYVGAHNAAVYSEFLGLDED
                     DIAELKQAEAI"
     gene            9599..9814
                     /locus_tag="AC16VR_04765"
     CDS             9599..9814
                     /locus_tag="AC16VR_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010499.1"
                     /translation="MSAYSIAREHLDVVASLAADNGVDLEAALRAVIGEAVERYRDLK
                     GADDLRAVLQYQLDNASGNEDYEFMRP"
     gene            9914..>10818
                     /gene="ribB"
                     /locus_tag="AC16VR_04770"
     CDS             9914..>10818
                     /gene="ribB"
                     /locus_tag="AC16VR_04770"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HIR6010500.1"
                     /translation="MSTQFDTIEDALAAIRSGEMVVVVDDDDRENEGDLIMAASKATS
                     EQVAFMIRHTSGILCAPILEDHAKQLHLDPMVARNDAPMSTAFTVSIDYRQGLTTGIS
                     AEERAATVQALTSSNVAAEDFVRPGHIFPLVARQGGVLVRSGHTEAGTDLAGLAGLPP
                     VGLLAELVNDDGTVQRLPELKEFAATHGLKIISIADLIAYRQMREQLVERTMEFAVHT
                     RIGEARAVAYKTKFEDAEHIALVFGEIGDEAVPVRIHREKLIDDIFGPQSNHPLGLLD
                     VALQRIQDLGGGVLIYLRSGFVGVPL"
BASE COUNT         1746 a         3475 c         3689 g         1908 t
ORIGIN      
        1 ccgtcggcga acgtgcacca gtacttgctg ccggtgatga cccactcgtc cccatccagc
       61 acggcccggc acttggccgc agccagatcg gagccgacgt ccggctccga aagggagatc
      121 gcggccagat actcgccccg cgcggccttc tgcgtcagct cctcgcgctt ctcaaggggc
      181 cagccgccca cgttcatgag cgacgtggcc cgggcgatga tgctcgccac gctcatccag
      241 ccccgcgcca gctcctcgct gatcaggcag tactcgaaca cgccgagccc caggccgccg
      301 tgctcctcgg ccatgcggat gccgaaatag cccatctcgc cgagcttgtc gcgcaggtcc
      361 atggggatgt ccccctggat ggggtcgagc tcgttggcca cgggcagcac ttctcgccgg
      421 gtgaagtccc gggcgacctc ctgaatcatg cgtcgctcgt cggtgaggta agtctcggtc
      481 attggctaca tcccaatcag ttctgcccga tcgagcaggt tctgcacgat tcggatactg
      541 gcggcgtcca ccatcacgcc gtccacgttg atggctccca gcccctggct cagggcctcg
      601 ttgtaagcgg ctttctgttt gcgggcgtcg gcgatggctg ccgggtccgg tgtgaaaacc
      661 gcgttggcga gctcgatctg gctcggatgg atggcccact tgccgaccat gccgagcacg
      721 aaggcgcgct cggcctcctt gccgtaggcc tcggagttgc gaaagttggc gtaagggccg
      781 tccaccgggt cgataccgtt ggcgtggcag gcgatggtca tcttgtagcg cgggtagtgc
      841 cagacgtctg gcgggtagcc tcccgagctt ccgatgtggc cgagctgcat ctgctggctg
      901 gcggagtagt cgcccatccc gaacacgagg cactcgagcc gatcggagca ggcggcaatc
      961 tcctcaacgt gcatcatgcc ctccacctcc tcgatgaggc actcgatgcc gatgcggcgc
     1021 gtaagcccca gcttctgctc cagctggtcg agcatcaggt ggacgaacat gacgtccgcc
     1081 gcgctcttgg ctttggtcag catgatcgtg tccagcttct caccggctcc ggtgaccacc
     1141 tcgatgatgt cgtcgtggca ccactgggtc tccacgtcgt tgatgcgcac gcagcgggtg
     1201 cgcggcgtcc agtggagccc gttcagcgcg tccacgatca tgccccgggc tgcgggtttg
     1261 gcgctggggg ccaccgcgtc ctcgaggtcg aggaacacgt gatccagatc cagggccgcg
     1321 gccttggcca tcatcttctc ggagctgccg ggtacggaga gctggcagcg cctggcgcgt
     1381 ttgggttgtc ggttcattgc ggtatcgctc cgtagtggct cttgcgtttg agcatgacgg
     1441 tacgttctcc ctcgaaaacg actttgtcgt cctggttggt tccccagtgc ctgaaggtca
     1501 cgatgccggc atcctcccgg tcggcctccg cggtttcgag cacctcggtg taggcgtaca
     1561 gcgtgtcgcc atgaaaaacg ggcgccttca ggcggatctt gtccagcttc agctcccgca
     1621 aggcgttctc gcccgtatcc tgcatggtga ggccaatcac catggcgatg gtcacgccac
     1681 cgaactgcag gcgcgagttc cagccgccgc ggccgtcccc cagagccagc gcgttgcgct
     1741 gcatgacgtc ctcgttgaag tgcccctcgg cggtgttgag cacctgcagg gtgatcccga
     1801 cgttgtcctg ctcgcacatg gtcttgcccc gggcgtgccg gaaaacctgg ccggcctgga
     1861 agtcctcgaa atagttgcgc tcgccggtca acgccatggt caatcccgct tgcgcttgcg
     1921 gaccaggttg gtccggtgat agtccagcac tttctccccg tgctggttga agccttcggt
     1981 gtgccaggtg acgatgccga aagccggttg gcccttcgaa tcgcgcgcgg tcaccaccgt
     2041 gctgcgcgcg gtgagcgtgt cgcccacgta gacgtccgcc agaaattcgc agtcgtccac
     2101 gcccaggaac ggaccgcccc cctcgctcaa atcctccacc gtgagaccga aaacggtgtt
     2161 gaagaccagc aggggattga ccacggtccc cgggtggccg agggatcggg catagggctc
     2221 gttgaagtag agcgggcaga aggaaagggt ctgggtggta aacgccgcgt tgtccccatc
     2281 ggtgatcgtg cgcccccagt ggtggtcgaa cacccggccc tcggtcatgt cctcgtagcg
     2341 gttacctttg ctaagcagca cggcccgatc gcggaagtcg ttcgtttcgc tcatcgatga
     2401 acccgcagca ggctgaagaa gtcgcccaga tcgtcctggg agtccagggt cctgaccagg
     2461 ccggcgagtt cgtccgcatg ggcacggccg acgcggtcgt ccaccagggc gtggaatttg
     2521 tgctctaact tctcccattg ggcatccagg tctcgctcag ggatcgccac gttgacggcc
     2581 tctcggtggg tgctcccgtc ccgggtggtg atggtgacga tactgtccgg gttgggcgtc
     2641 tcgtgggcga ccacgcggac cttgtcccgg aaggccacca gctcgggttg ctgagtcagc
     2701 gcgtcggtga agatctcgat gctcgccgtg tcgacgccgg caagggccat ggccgccgtg
     2761 aagcgcaggc tgaacttggc ctccaggccg gatcggggct cgaggatgtt gcacacccga
     2821 aaatgaccgg catccacccg gatctcgatg ctttcgatgt cgttcgggtc gaaggcgttg
     2881 cgccggcaga gattgttgac cgcctcgatg gaggagtggg tcaggtagca ggccgcgtgg
     2941 tacttgaagc agatgtcctg ggtgtagccg tcccgctcca tggcccgggc aaagccctcc
     3001 tcgcaggcgc cgctggcctg ggtcgccatg aagccctgct ccacctcgag gatgtcccgt
     3061 cggctgtcga agccccgctg cgccaggctg gctgattgaa cgccggtggc ggcagcgtgg
     3121 ccggcgtgca gcggtttgca catggtcccg aactgagatt tcaaccccgc ggcctgagtg
     3181 ccggcgatgc cgagggcgcg gctggtggca tcctcgtcca gacccatgat gcgggctgcc
     3241 gcggccgcgg ctccgaagct gcccagggtt ccggtggcgt gccagccatg ggcgtagtga
     3301 ccgggcccgg cgtagtggcc gaggatgcac tcggtatcca cgcccgcgca gaacgcggcg
     3361 atggcctctt ctccggaccg acccagccgc tcggcgagag cgagcacggc gggcgcgacg
     3421 ggcacggtgg gatgtccggt catggccgta tgcacgtcgt catagtcgtg agcgtggccg
     3481 gctgagccgt tgaccagggc ggcctggacg gtggacgccc gctcgtcggt gccgatgagc
     3541 tgtgcctggg ggtgcccgcc ttcctcgttg acctgatcgc gcagcatgcg ggcgaggggc
     3601 tcgtcgatgc cggccatggt cacgccgatg aagtcgagca gcgcctgctt cgccaccgtg
     3661 cgtgccggcg ccgggatgtc ggcgtattga gtgccggcga tccgctcggc caacgcgcgg
     3721 gtcggtgtca tccgtggtcc tcccgaaagc ctccggacgg agactgttga gccctaagct
     3781 agcgggattg ccatggcttt gcaaagccgc ctgaggccct gttttagcgc cgccgggtac
     3841 aggctcggaa cgatttctgc ttcaatggcc gtctcccggc gaggcgaggc gaccccatgc
     3901 ccgagcgcgt ctattgcagc gagttcaatg gcgacgagcc catgctcggc acggcggacg
     3961 tggtggacgt ctggctgatg ctcgaatacc ggccggcctg gaaagccagg gccctggagg
     4021 agagcgcgct cgcggcgccg acgagacgct ggctggccgc cggcatcgat gccctggccg
     4081 ccgcggggct gcgggctcgc ccccagctcg tccgccaacc agagctcgac agagacacgg
     4141 tttcgctgct ggtcgccttc ggcgaccggt tgctcgaatt ccaggggaca ggctacgact
     4201 ttctgaacgg cgtggatctc gccgggctgg cgcggggcca tctgcccgaa ggcgctcgcg
     4261 tggtctcgga accgcgctac ttcgtctgca ccaacggcca gcgggatctg tgctgcgcgc
     4321 gcttcggcct tcccgtttac gccgcgatgc gggagcggct gggcagccgg gtgtggcagg
     4381 tgagccatct cggcggtcac cgcttcgcgc cgaacgtgct cgttctgccc caggcgaccc
     4441 tgtacggccg cgttcagcct gctgacgtgc ccgccttcat cgagcgcgtc gagtccggcg
     4501 aggtgtcgct tgcgcacctg cgggggcgca cggagtacgc gaagcccgtg caggcggccg
     4561 cggggtattg cgggggcggg gagctggatc tggtcgacgc ccggggcgac gatgacgccg
     4621 ccacggtgac gttccggcgc gggtccgagg agatcaccgt ggcggtggaa agatccgacc
     4681 ccgtggacgt gctcaagagc tgcggaaagg cgtcggagcc agcgtatccg tatcggagac
     4741 gctgaatcgc cgttcgccgc cgggcatctc gtcggcgatg aactcacgca gcgcgagggt
     4801 caacatcctc gcatcgccca cggggtcgaa cggctcggcg atgcggatcc ggtacggctg
     4861 gcgggttttg ttgagcagct cgcgaaacag cgtcatgtcc ttcagctcgg tgttgatggc
     4921 gtacagcaga tagtagagcc aggagttcct gccgcggatg tgcatgggga cgatggggca
     4981 tcggtacttc tgcgccaggt tcaccgccgt aggcgtccac ccgcgctcca ccaggcctcg
     5041 gagactgggc cgggccaggc gcccggaagg aaatatgacg atcagcctct cggatcgaaa
     5101 cgcgcggacc atgtggctga cggtctcccg gtttcgtgcg tgcgttcgct tgtcttccac
     5161 ccactcgacg ggcacgatca cgtcggcgag gcccggcgcg aagcgaatgg catcccgatt
     5221 ggcgaagaac gtgatgtccg acctcaccgt gcgcagcacg tcgaacacgg cgacgccgtc
     5281 ggcgatgccg gtggggtgat ttgctgtgac caccgcgctg cccgtttcgg ggatgtgctc
     5341 ggtgccgcca aggtcgatgt cgagcttcag ctgcgcgctg agcatctcga agatctccag
     5401 cccgcccatg tcggccaccg tatcggccat gcggaccgcc gcgcgatacc ccagcacggg
     5461 atacagcagg ccggtcacca gccgccacag cagggggtgc tgcatcagtc cccgggcccg
     5521 ttcctcgatg agcgtgtcga ctatgtgtgg catcggacga acgagctgac cgccgcgttg
     5581 aacggttcgt gggcttccat gttgacgccg tggccggctg gaatgtcgac gatctcgagg
     5641 cagggcatgg tcgcctgggc gaacgcgcga tggggctgaa agcgccgttc tctcgagccg
     5701 cagacgagca gagcgggacg ggtgttgcga tgcacgtccg cgcgcacgga cacgttcggg
     5761 ttggtgtggc gtagaacgtt cgcgacgccc gtcgggtcga gacgtgcggc gtccgccatg
     5821 agggcctggt gcagaggatc agggagcttg gtcgcgtgac ggggatgtac ggggatgcgt
     5881 tccagggcgg cctgacctgc ggcgatgatc cgctgcgcgg tctcgtccgc ctcgtcctgc
     5941 cagcgccgta tctgctcggc gtccgccagg ccggaggtcg agttggtgaa gacgtgtccc
     6001 atgacccgat ggggatgggt cagcgcgtag cggatggtca ggccggcacc gagggagtag
     6061 ccgagcacga accattgctc ggcgccgagg gccgtgcgga tggactcgaa ctcttccacg
     6121 atccgatccg gcgcgtagca ctccggatcg gacggtgctg gcgatcgacc gtggccccag
     6181 agctccacgg tgacggggcg gcagacctcg gccaggacgg tcaggttcca cagccattgg
     6241 gcgcggctgc acagaaagcc gtggaccagc agcaggtgcg gtcccttgcc atcgtgtatc
     6301 tgccagtcga gatgcatgta aacggctgcc cttgggatga cgcaaaataa gacattaacc
     6361 gatggcccgc catacggacg gtgtcgctcc gtcgcggacg ccggtgatag actgccacgc
     6421 aaggagggcg gcatggctta cgacattctg atcagaaacg gaacggtggt ggatggttcc
     6481 ggtggggcgc cttacccggc cgacgtgggc attcgcaacg ggcgtatcgc gtccatcggc
     6541 aggatccggg aaaaggcccg gactaccatc gatgccgagg ggcacgtggt cagcccggga
     6601 ttcgtggacg gccatacgca catggacgcc caggtgttct gggatcccat cgggtccagc
     6661 tcctgctatc acggcgtgac gagcgtggtc atgggcaact gcggcttcac cctggcaccg
     6721 tgccggcggg acgaggcgga cctggtcttc cgcaatctcg aacgtgccga agatctctcc
     6781 cgggacgcca tgctcacggg gatcgactgg cagtgggaga cctttccgca gtttctcgat
     6841 gcggtggacg gcctgcccaa ggggatcaac tacgccggct acatcggtca ttcggccctt
     6901 cggacctacg tcatgggcga gcgcgctttc accgacccgg ccaccgacgg ggatctggcg
     6961 gccatgtgcc ggcaggtgca ggaagccgtg cgcgcgggcg ccatcggctt ctcgacgtcg
     7021 cgcacgttca accacatcac cgccgacgac cgtcccgtgg ccagccgtct ggcagagtgg
     7081 gacgaggtgc gcgccatcgt caacgccatg ggcgagacgg gcaaaggcat cttcgagatc
     7141 gccggcgaag cgccgggccg tgatccggct cgaattcgcg actaccacga acggcttcgc
     7201 gacctggccg tggagtccgg ggtgacccag acctggggca tgttcagcgt gcgcatcgcg
     7261 cccgatttct ggcgacccta tttcgatctc ttggacgaga ccgcggcagc gggagggcga
     7321 atgtacgccc aggtgcacag ccgggcgctc aacgtgctgc tgtcgttcaa gacctcgacg
     7381 ccgttcgaca agtgggacgt ctggaatgac gtccgctcgc tccccctgga cgagcagaag
     7441 gcgaagcttc gcgatcccgc cctgcgcgcc cggctggtcg aggccgccaa tcgcgagtac
     7501 cagggccctc gtgtggtggg cgccgaagcc aggccgccga actgggagtg ggtctacccc
     7561 atggcggaca tgagctacga tcagcccagc atggccgagc tggccgccga gcgcggcgtg
     7621 gatcccgtgg agctcatgat cgacatgtcg ctggcgcacg acttcgacat cttcttccgt
     7681 cagcccatcg ccaacgaaaa tcaggacgac gtgctggaaa tgatgaagca tccgcgggcg
     7741 atggtgacct tctccgattc cggcgcccac gtctctcaga tcatggacag ctccctgcag
     7801 acccacctct tgagctactg ggtgcgggag aagcaggcgt tcaccctgcc cgaggcggtc
     7861 cggaggatca cctacgatac ggccaccatg tggggcctgc acgatcgcgg tctggttcgg
     7921 gcaggcatgg cggcggacct ggtgattttc gaccccgaaa ccatcggccc gcggatgccc
     7981 accgtggagc acgacctgcc ggcgggcgct cgccggctca agcagacggc cgacgggatc
     8041 atgcacacca tcgtcaacgg cgaggtgctg ctgacccgca acgagtacgg cggagcgacc
     8101 ccgggcaggc tgctgcgcgc ctgagctcgc tcgagcaggc ggccgctcgg ggctgccagg
     8161 aagaagggat ctggcgcggc tgggaggact cgaacccccg acacctgggt tcgaagccca
     8221 gtgctctatc cagctgagct acagccgctc tggaagagtc ggtcagtata accgtatcca
     8281 cctgggcggg cgctgccttt tcccgtgagc agtggtggct ggccagcaaa ccggctaccg
     8341 ttcgtgcaga gacgatgctg gtggggtgcg cgatgaacga gctgccgctg gtcggtgtga
     8401 ccgtgctgga tttcggccag gtctacaacg gaccgtactg cgggttcctg cttgcccagg
     8461 cgggcgcgcg ggtcatcaag gtcgagtcgc cgcggggcga gaccctgcgc agccggggcg
     8521 agacgaccgg cgcgagctac ccgttcgcca tgctcaacgg gaacaaggaa tgcatctcgc
     8581 tgaacttccg ctccgctcgc ggccaggcgc tgctgcggga cctggtcatg cacgtcgacg
     8641 tggtgctgga gaatttcgcg cccggtacgc tggagcgcta cggggtgggg gccagaaccc
     8701 tgcgtggcct caaccccgag ctcatctacg cgtcgagcac gggctacggc cggtcgggcg
     8761 cgcaccgcga ctaccgcggc atggacgtca ccattcaggc gatgacgggg gtcatgagca
     8821 tcaccgggga gggcgacgga cctcccctga aggcggggcc tgccctggcg gacttcctgg
     8881 gtggcgtgca cctctacggc gggatcgtca ccgcgcttta tcgtcgcagc cagaccggtg
     8941 agggtgcggt catcgacatc tccatgcagg actgcgtctt tccttccctg accaccgcgc
     9001 tgggcgccta cttcgtggcg ggcgggcagc agggtccgcg cacggggaac cggcaccagg
     9061 gcctgagcgt cgccccctac aacgtttacg ctgcccggga tggctatgtc gccatcatct
     9121 gcatacgcga gggccactgg cgtgggctga cgcgggcaat gggtcgcccg gagctggccg
     9181 aggatgcgca gtttgcggac atgcgtgcca gggcgcagaa catggatgcg gtggacgcgc
     9241 tggtggaggg ctggacccgg accctcgacc gggaagagat ctttgcccgg gcccaggccg
     9301 aaggcgtgat ctgcgcgccg gttcagacgc tggaagacgt gatcaacgat cctcacctcc
     9361 acgagcgcgg ctcccttcac tggcacgaac atccgcgcct gggtcccatc gtccttttca
     9421 actcacctct gcggttcgaa ggcgtggatc tgccggaggt tgcggaagtc ccttatgtgg
     9481 gcgctcacaa cgccgccgta tactcggaat tcctcggtct cgacgaggat gacattgccg
     9541 agctgaagca ggccgaagcg atctgatcga aagcttcgtt gaaagtaagg aggttggttt
     9601 gagcgcctac agcatcgccc gtgagcactt agatgtcgta gcttctctgg cggcggacaa
     9661 cggcgtggat ctcgaggctg cacttcgcgc ggtcatcggc gaggcggtgg agcgttaccg
     9721 ggacctcaaa ggcgccgacg atctcagggc cgttctgcaa taccagctcg acaacgcttc
     9781 cggcaacgag gactacgagt tcatgcgccc ctgaacgggc cattcgatac ccttccccgt
     9841 ggcgtggtaa tctagcgcgc tcgtggcaat ccccgggtcg ttcgaacccg ttgtgaatta
     9901 aggtcaagcg cagttgagca cgcaattcga caccatcgaa gatgcccttg ccgccatccg
     9961 ctcgggcgag atggtggttg tggtggacga cgacgatcgg gaaaacgaag gcgatctgat
    10021 catggccgcc agcaaggcga cgtcggagca ggtcgcgttc atgatccgcc ataccagcgg
    10081 tatcctgtgc gcgcccatcc tcgaagatca cgccaagcag ctgcatctgg atcccatggt
    10141 ggcgcgcaac gatgcgccca tgagcacggc tttcacagtt tccatcgact atcgacaggg
    10201 cctcaccacg ggcatatcgg cagaggagcg ggcggccacc gttcaggcgc tgacctcgag
    10261 caacgtggcg gccgaggact tcgttcgtcc cggccacata ttcccgctgg tggctcgtca
    10321 gggcggcgtt ctggtccggt ccgggcatac cgaggcgggc acggacctgg ccggtctcgc
    10381 cggcctgccc cccgtgggcc tgctggcgga gctggtcaac gacgacggca ccgtgcagcg
    10441 cctcccggag ctgaaggagt tcgccgccac ccacggcctg aagatcattt ccatcgccga
    10501 cctcatcgcc taccggcaga tgcgcgagca gctggtggaa aggacgatgg agttcgcggt
    10561 gcacacccgc atcggcgagg ccagggccgt ggcctacaag accaagttcg aggacgccga
    10621 gcacatcgcg ctcgtattcg gcgagatcgg cgacgaggcc gtacccgttc gcattcaccg
    10681 ggaaaagctc atcgacgaca tcttcggccc gcagagcaat cacccgctcg gccttctgga
    10741 cgtcgctttg cagcgaatcc aggatctggg cggcggcgtg ctcatctacc tgcgctcggg
    10801 cttcgtcggg gtgccgct
//
LOCUS       DBNHQR010000208         2976 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1413488, whole genome shotgun sequence.
ACCESSION   DBNHQR010000208 DBNHQR010000000
VERSION     DBNHQR010000208.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1413488"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="AC16VR_04775"
     CDS             complement(<1..641)
                     /locus_tag="AC16VR_04775"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR6010501.1"
                     /translation="MRTARFSVCMGARMNEGDRAGGSTARGAGVAFGLVLALLGSQAS
                     ASAIEEIVVSARAVEQSVRDVPVAITAFNEEVMDNMALTSFDDIAAYTPSVDILRISS
                     GNGTSVSIRGISSSAGSLGIEQSVAIVIDGVYYPQGRSINEGLFDTSQVAILKGPQAL
                     YFGKNATAGVISVMTNDPGDELEILGKIGYETELKTTRAEGVLSFPINDRWGVR"
     gene            complement(748..1974)
                     /locus_tag="AC16VR_04780"
     CDS             complement(748..1974)
                     /locus_tag="AC16VR_04780"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6010502.1"
                     /translation="MEKSEFFADARRDLTGPIHGIRVLEITTSWAGPMAGCMLADLGA
                     EVIKVEHPDGEIGRVLGPHLPGGAHLSLLYETANRNKRNLSVDMRKEVGRDLLIDLGR
                     TCDLVVENFKPGTLHGWGLGYEHFCAAKPDIVYVSISGFGQFGPHQARAAYDPLVQAQ
                     SGWMSLNGEIGGGPVKAPTFLGDDLAGMQAALAALAALRHRDATGEGQHVDVALMDAI
                     LGASNMFPSMARLGIEAPRTANQFPQVAPFNVYACRGGSVFLGMSLNSHWEIFANAVG
                     RPELVEDPRYATTELRTQNRESVDELVSRWCSEQDQMRVVELLGGAGLPVAPVNDYRA
                     ATDDPNIEARDMLTDIELSDGSRAPLLGPVPKFSRTPIGVRSGAPTIGQDNASILDEL
                     GIDDARRRALREAGVI"
     gene            complement(2007..>2976)
                     /locus_tag="AC16VR_04785"
     CDS             complement(2007..>2976)
                     /locus_tag="AC16VR_04785"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6010503.1"
                     /translation="YQLDGTLEEVPCAWEFPHLDYEANRLPEVLAESWNGFVFINMDQ
                     DAQPLLQYLEVLPEHFASWRFDDWYVASHVRKELHCNWKVAMEGFMEAYHTPVVHPEM
                     CQVVGDWNMQHDIFGDHVSRDLCPMGVSSPSLDEPLSEFELLRRARPGNPLTSETGSA
                     ELPDGKTARMVMAEETRRSFRDQFGMDLSDLSDAEVIDSLKYNVFPNLMFSGGAAIHG
                     LSIFRPLGYDTNKCTIDRLTFLPVPKDGRRPPPAEVITIAEGESYATADTLNEFTAHV
                     LDQDTSIMRLQQEGMHASAKGAETLSTYQESRVRWLHETLEKYLSR"
BASE COUNT          519 a          996 c          936 g          525 t
ORIGIN      
        1 cgcacacccc atcggtcgtt gatggggaaa gacaggaccc cctcggcgcg ggtggtcttg
       61 agctccgtct cgtagccgat cttgcccagg atctccagct cgtcgccggg atcgttggtc
      121 atcaccgaga tcaccccggc cgtggcgttc ttgccgaaat acagcgcctg gggccctttg
      181 agaatcgcca cctggctggt gtcgaagagg ccttcgttga tggagcggcc ctgggggtag
      241 tagaccccgt cgatgacgat cgcaacggac tgctcgatgc ccaggctccc ggcactggag
      301 gatatccccc ggatgctgac ggacgttccg ttgccactcg atatgcgaag gatgtccacc
      361 gatggggtat atgccgcgat gtcgtcgaag ctggtcagcg ccatgttgtc catgacctcc
      421 tcgttgaacg ccgtaatggc caccggcacg tcccgaacgg actgctccac ggcccgggcg
      481 gaaaccacga tctcctcgat ggcgctggca gacgcctggc taccgagcag cgccaggacc
      541 aggccgaaag cgacgccggc cccacgtgcg gtcgacccgc cagctctatc gccctcgttc
      601 atgcgcgcac ccatgcacac agagaatctc gccgttctca tatctaccct ccccagggat
      661 ggattcaact cttttgatta tgtgcgcctt ccggacctgc aggcaaccac cccgacgctt
      721 cggctcacgc agcagcgacg gccccgttca tatcaccccg gcctcccgca acgcccggcg
      781 ccgcgcgtcg tcgatgccga gctcatcgag aattgaggcg ttgtcctgcc cgatggtcgg
      841 cgcgcccgag cgcacgccga ttggcgtacg ggagaacttc ggcaccggcc cgagcaacgg
      901 agcccggctg ccatccgaga gctcgatgtc ggtcagcatg tcgcgggctt cgatgttggg
      961 atcgtccgtc gcggcgcggt agtcgttgac cggcgccacg ggcaggcccg cgccgccgag
     1021 cagctccacc acgcgcatct ggtcctgctc gctgcaccac cgcgaaacaa gctcatcgac
     1081 gctctcccga ttctgcgtgc gcagctcggt ggtcgcgtaa cggggatcct cgaccagctc
     1141 cgggcgcccc acggcattgg caaagatctc ccagtgggag ttcagcgaca tgccgagaaa
     1201 gaccgagccg ccgcggcagg cgtagacgtt gaagggcgct acctggggaa actggttcgc
     1261 cgtgcggggc gcttcgatgc cgagccgcgc catggacggg aacatgttgg aggcgcccag
     1321 gatggcgtcc atcagcgcaa cgtccacgtg ctggccctca ccggtggcgt cccgatgtcg
     1381 aagcgcagcc agcgcggcca gcgctgcctg catcccggcc aggtcatcgc ccaggaaggt
     1441 gggcgccttg accgggccgc cgccgatctc tccgttcagg gacatccacc ccgattgcgc
     1501 ctgcaccagc gggtcgtagg cggcccgtgc ctggtgtggc ccgaactgac cgaacccgct
     1561 gatggaaacg tagacgatgt cgggctttgc cgcgcagaag tgctcgtaac ccaggcccca
     1621 accgtgcagg gttccgggct tgaagttctc caccaccaga tcgcacgtcc ggccgaggtc
     1681 gatgagcagg tcgcgaccaa cctccttgcg catgtcaacg ctcaggttgc gtttgttgcg
     1741 gttggcggtc tcgtaaagaa gagaaaggtg agcgcctccc ggcagatgcg ggcccagaac
     1801 ccggccgatc tcgccgtccg ggtgctccac cttgatgacc tcggcaccca gatcggcgag
     1861 catgcagccg gccatcggcc cggcccagga tgtggtgatc tcgagcacgc ggataccgtg
     1921 gatgggaccg gtcaggtcgc ggcgagcgtc cgcaaagaac tccgacttct ccaaactagc
     1981 ccccttgcgc tgctgcggct gcgccgctac cgggaaagat acttctccag cgtctcgtgg
     2041 agccagcgaa cgcgggattc ctgataggtc gagagcgtct ccgccccctt ggccgaagcg
     2101 tgcatgccct cctgctgcag ccgcatgatg ctggtgtcct ggtcgaggac gtgggcggtg
     2161 aactcgttca gggtgtccgc cgtggcatat gactcgccct cggcaatggt gatcacctcc
     2221 gccggcggcg gccgccgacc gtccttcggg acgggcagaa acgtcaagcg gtcgatggtg
     2281 cacttgttgg tgtcgtagcc gagggggcga aatatgctga gcccgtggat ggccgcaccg
     2341 ccggagaaca tcagattggg aaagacgttg tatttcagag aatcgatcac ctccgcatcg
     2401 ctcagatcgg agaggtccat accgaactga tccctgaaac tgcgtcgggt ctcctcggcc
     2461 atcaccatcc gggccgtctt gccgtccgga agctcggctg atccggtctc cgacgtcagc
     2521 gggttgcccg gccgcgcgcg ccttagcagc tcgaactcgc tcaacggctc gtcgagagac
     2581 gggctcgaga cgcccatggg acacagatcg cggctgacgt ggtctccgaa tatgtcgtgc
     2641 tgcatgttcc agtcgccgac cacctggcac atctcggggt gcaccacggg cgtgtggtag
     2701 gcctccatga agccttccat ggcgaccttc cagttgcagt gaagctcctt tcggacgtgg
     2761 ctcgccacgt accagtcatc gaagcgccag ctcgcgaaat gctcgggcag cacctcgaga
     2821 tactgaagca atggctgcgc gtcctggtcc atgttgatga acacgaagcc gttccaggac
     2881 tcagccagca cctccggcaa ccggttggcc tcgtaatcca gatgaggaaa ttcccaggcg
     2941 cagggcacct cctcgagagt cccgtcgagc tgatag
//
LOCUS       DBNHQR010000209         3351 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_590704, whole genome shotgun sequence.
ACCESSION   DBNHQR010000209 DBNHQR010000000
VERSION     DBNHQR010000209.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_590704"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="AC16VR_04790"
     CDS             complement(<1..314)
                     /locus_tag="AC16VR_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HIR6010504.1"
                     /translation="MTRQIVLDTETTGLEAQKGHRVIEIGAVEMIDRKPTGRHFHKYI
                     NPDRDIDDGAFEVHGLSSEFLSDKPRFEDIAQEFLAFVSGAELVIHNAGFDVGFLNYE
                     LA"
     gene            complement(311..763)
                     /gene="rnhA"
                     /locus_tag="AC16VR_04795"
     CDS             complement(311..763)
                     /gene="rnhA"
                     /locus_tag="AC16VR_04795"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HIR6010505.1"
                     /translation="MSAPVEIFTDGACRGNPGPGGWAALLRYGDAEKLISGAAPDTTN
                     NRMELQAAISALEVLTRPSSVAITTDSEYLRQGINQWIQKWKRNGWRTSQRKPVKNRD
                     LWQKLDELVQQHQIEWHWVRGHSGHPENEQVDEAANVAIDRMLTGGES"
     gene            complement(747..1529)
                     /locus_tag="AC16VR_04800"
     CDS             complement(747..1529)
                     /locus_tag="AC16VR_04800"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6010506.1"
                     /translation="MSAWLATPVGERLLADEAPYVAETARRFHGDTLLWAGCHTPLTD
                     TVRGCMVRNRVFALDGSSPAGEGLTTFRCELDAIPLANNALDGVLLHHALEMAEDPRT
                     ALRELSRVLAAGGRLTVVGFNPFSFWGVRIAYARVFRDSFSGLRLVSPARLVDWLTVL
                     GFELCEEVRFTAYNLPFRLGNTGEQDERSTQQLIPSSWGPRLRNALDHLNPPVGGVYM
                     VSAVKQALGVRRDWRLSAAREPKLAPVAYPKLSARNRIERSR"
     gene            1604..3151
                     /locus_tag="AC16VR_04805"
     CDS             1604..3151
                     /locus_tag="AC16VR_04805"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase"
                     /protein_id="HIR6010507.1"
                     /translation="MTATAPVVRFASTLRTLMFVILASLVGCQTNPIPEAAETAAAEP
                     PPPVAVPPPVIEPTLTYELKPDLWRDIRERFELAHSLDQKRVQQELRWLRENPRYLHN
                     LKPRMERFLAYIHGEVAARELPGELALLPIVESALDPYAFSHGGAAGLWQFIPATAKR
                     FGLQRNWWYDGRRDPIAATQAALDYLEYLHLLFEDWHVAVAGYNAGEGNVRRAQRKAA
                     PGATFWEMALPRETQMYVPRLLALAAVVTDPEAYGLTLPELSPDVPFVAVDTQGQFDL
                     AKAADTLSMDLDALYDWNPALNQWSTPPDGPHRLMIPALEPALSQAALAAVPAGERVQ
                     WLRIKVADGDTLSEIAHRHRTDTATLRRVNNLTGSSIRAGQAMYIPKSDSAPSRSPRS
                     RAQKGLEYAVQPGDSLWTISRAHGVSMTRIMKANHVGPKDLLKVGQTLTIPGAQRSTP
                     AAGEDGREVIRTVRYGVRKGDSLARIAARFNVRVSEIAQWNQLDVDNYLQPGQSLKLH
                     VNVAAGQ"
BASE COUNT          621 a         1133 c         1019 g          578 t
ORIGIN      
        1 attgccagct cgtagttcag aaatccgacg tcgaaacccg cattgtggat gacgagctcc
       61 gcacccgaaa caaaagcgag aaactcctgc gcgatgtcct cgaaccgggg cttatcgctg
      121 agaaactccg agctcagacc gtgcacctcg aaggcgccgt catcaatatc gcgatcgggg
      181 ttgatgtact tgtggaagtg acggccggtc ggcttgcggt cgatcatttc cacggcaccg
      241 atctcgatga cccgatgccc cttctgagct tcgaggccgg tggtctcagt gtcgagaacg
      301 atctgccgcg tcaagattcg cctcctgtga gcatgcggtc gatggccacg ttcgccgctt
      361 cgtccacctg ttcgttttcc ggatgcccgc tgtgcccgcg tacccaatgc cactcgatct
      421 ggtgctgctg caccagctca tccagctttt gccagagatc gcggttcttg accggctttc
      481 gttgagacgt tcgccagccg ttgcgcttcc atttctgaat ccactggttg atgccctgcc
      541 tgagatattc agagtcggtg gtaatggcca cgctgctcgg ccgcgtgagc acctcgagcg
      601 cggagatcgc cgcctgcagc tccattcggt tgttggtcgt gtccggagca gcaccgctga
      661 tgagcttctc ggcatcccca taccgcagca gagcggccca gccgccaggc cccggattgc
      721 ccctgcaggc cccgtcggtg aagatctcaa cgggagcgct caatccggtt ccgcgcgctc
      781 agtttcggat acgccacagg ggcgagtttc ggttcccggg cagcagacag acgccagtcc
      841 cggcgaacgc cgagcgcctg cttgaccgcg ctgaccatgt aaaccccgcc gaccgggggg
      901 ttgaggtgat ccaacgcgtt tcgcagccgg ggaccccaac tcgacggaat cagctgctgc
      961 gtcgaccgtt cgtcctgctc gccggtattt cccaatctga acggcaggtt gtaggcggta
     1021 aagcgcactt cctcgcaaag ctcgaaaccg agcacggtca gccagtccac aagccgagcg
     1081 gggctgacca gccgcagccc gctgaagctg tcgcgaaaga ctcgcgcgta agcgatccga
     1141 acgccccaga aactgaacgg gttgaacccc acaaccgtca gccgcccgcc ggccgcaagc
     1201 acgcgggaga gctcccgcag cgcggtgcgc gggtcctcgg ccatctccag cgcatggtga
     1261 agcagcacac catccagggc gttgttggcc agaggtatgg cgtccagctc gcagcgaaag
     1321 gtcgtgaggc cctcgcccgc cgggctcgaa ccatcgagcg cgaacacgcg attgcgaacc
     1381 atgcatccgc gcacggtatc agtcaggggg gtatggcacc ctgcccagag cagcgtatcg
     1441 ccgtgaaagc ggcgggccgt ttccgccacg tagggcgcct cgtcggcaag gaggcgctca
     1501 ccgaccggtg tagccagcca ggcggacatc tgatgacgca aggatcttgc caatcgcaca
     1561 gggataccgc aaagttgaag gatcattctg aaggcaccac tcgatgaccg caaccgcacc
     1621 tgtcgttcgg tttgcctcga ccctgcgcac gctgatgttt gtgatcctgg cgtcgctcgt
     1681 ggggtgccag accaatccca tccccgaagc ggccgaaacc gcggccgccg agcctccccc
     1741 gcccgtcgcg gttccgcctc ccgtcatcga gccgacgctc acctacgagc tgaaacccga
     1801 tctgtggcgc gacatccgcg agcgctttga gctggcccac agcctcgacc agaaacgcgt
     1861 gcagcaggag ctgcgctggc tgcgcgagaa tccgcgctac ctgcacaatc tgaaaccccg
     1921 aatggagcgg ttcctcgcct acattcacgg ggaggtggcc gctcgggagc ttcccggtga
     1981 gctcgccctg ctccccatcg tggagagcgc gctcgacccg tatgcgtttt ctcacggcgg
     2041 cgccgccggt ctgtggcagt tcatccccgc cacggcgaag cgattcgggc tgcagcgaaa
     2101 ctggtggtac gacggccgtc gggatcccat cgcggctact caagccgccc tcgactatct
     2161 cgaatatctt catctgcttt tcgaggactg gcatgttgcc gtcgcgggct acaacgctgg
     2221 agagggcaac gtccgccgcg ctcagcggaa agccgcaccg ggggccacct tctgggagat
     2281 ggctctgccc cgggaaaccc agatgtacgt gccgcgcctg ctggcgttgg cagcggtggt
     2341 aaccgacccc gaggcttacg gcctgacgct gcccgagctt tcgccggacg tgccgttcgt
     2401 ggccgtcgac acgcagggac agttcgacct cgccaaggca gccgacaccc tttccatgga
     2461 tctcgacgcg ctctacgact ggaatccggc cctgaaccag tggtcgacgc cgccggatgg
     2521 tcctcaccgg ctgatgattc ccgcacttga accagcactg agccaggcgg cccttgcggc
     2581 cgttcccgct ggcgagcggg tccagtggct gcggatcaag gtggcagacg gagacaccct
     2641 cagcgaaatc gcccaccgtc accgaacgga cacggccacc ttgcgacggg tgaacaatct
     2701 gacgggctcc tcaattcgtg ccggccaggc tatgtacatt cccaaatcgg attcggcccc
     2761 cagccgcagt ccccgcagcc gcgctcagaa aggactggaa tacgcggtgc agcccggaga
     2821 ttccctgtgg accatcagtc gggcgcacgg ggtttcgatg acgcgcatca tgaaagccaa
     2881 tcacgtgggg cccaaggacc tgctgaaggt aggccagact ctcaccattc ccggcgctca
     2941 gcgctcgacg cccgcggcgg gagaagacgg acgggaagta attcgcacgg tgcgctacgg
     3001 cgtgcgcaag ggcgactcgc tggcgcgcat cgccgctcgc ttcaacgtgc gggtcagcga
     3061 gatcgcgcag tggaaccagc tggacgtgga caactacctg cagcccggcc agtcgctgaa
     3121 actgcacgtc aacgtagcgg ctggacagta actgaccagc cggccgccgg acggggtcga
     3181 cctacggcgt gctgatcgac gcttcccttt ccagagtggt cgtcagcccc gcgaaatcca
     3241 gagaagccac gcgatttccc aggaagccgc gcatgctttc gatctctctt tccgccatca
     3301 cggaaacatc cccgtcgacc acacgggtaa cggtgaccac cgcaacggag c
//
LOCUS       DBNHQR010000210         2742 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_717104, whole genome shotgun sequence.
ACCESSION   DBNHQR010000210 DBNHQR010000000
VERSION     DBNHQR010000210.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_717104"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="AC16VR_04810"
     CDS             complement(<1..834)
                     /locus_tag="AC16VR_04810"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HIR6010508.1"
                     /translation="MGGDAIDLGTMLLGLLGGLAIFLFGLDQLTNMLKVTAGARMGDL
                     LAKMTTNRFKGVLAGGVTTAVIQSSSVTTVLVVGFVSAGLMTLQQSIGVIMGAEIGTT
                     ITAQIIAFKITEYALVAVVVGFVMQAFFDNDLVKRYGTMVFGLGLVFFGMFMMGEATR
                     PLRDYPPFVDLLAQMDNRVLTILLSAGFTALIQSSSATTGVIIVLGSQGLISLEQGIA
                     LVFGANIGTCVTAVLASIGKKHRGLQVALVHVTFNVGGVLMWFAFIDELAAFSRWLSP
                     AS"
     gene            complement(909..>2742)
                     /locus_tag="AC16VR_04815"
     CDS             complement(909..>2742)
                     /locus_tag="AC16VR_04815"
                     /inference="COORDINATES: protein motif:HMM:NF013199.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR6010509.1"
                     /translation="RDEEHVAIVTYLLEANGWLDATSPFDFSTPRRIGGGADPEPDSG
                     EALDSWGEASSIDAIARSRSAFVNRKVQKYVPVTDAMLASPPAGDWLSWRRTLDGQGY
                     SPLDDINRETVGKLKLAWVLAMRDGSNQVTPLVHDGVMYLTHARNLIQAVDGSSGDVI
                     WEYAYEYPEAARTLGGPLRNIAIYGDKLFMATYDAALVAVDARTGEQVWRAVKADYRQ
                     GYTHTSGPIVGDGVVLSGINGCERYKEDGCFVTGHDADTGRELWRTSTIALPGDVNDA
                     SWGGLEPSFRAGGDTWIAGSYDPKLKLFFIGTSQAKPWVAASRGMTVDDAALYTNSTL
                     AMDPRTGKIVWYFQHVPGETIDMEVGFERVLVDVEGARLLFTVGKDGILWKLDRATGQ
                     YVAHVETIEQNIFEHVDPETGRVRYREDIRSAGVGETFSACPGIYGGHNWQAMAYSPQ
                     ARSLVIPLHRLCADMVGRPVESKVGEGGFGGDSRSYEMPGSNGNLGRLVAVDAATLEE
                     RWVHEQRAMFMTGALTTAGGLVFIGDLDRYVKAFDVADGRLLWQARLGAPTHGYPVTY
                     RAGGKQYLAVPTGMGVFRALTAVISPDIYQPANGQALYVFELPN"
BASE COUNT          525 a          918 c          838 g          461 t
ORIGIN      
        1 agacgcagga gacagccatc gcgagaaggc ggcaagctcg tctatgaagg cgaaccacat
       61 cagcaccccg ccaacgttga aggtgacgtg gaccagggcg acctggagcc cgcggtgttt
      121 ctttccgatg gaagccagca cggcggtcac gcaggtgccg atgttggcgc cgaaaaccag
      181 agctatcccc tgctcgaggg aaatgagccc ctggctgccc agcacgatga tcacgcccgt
      241 ggtcgccgac gagctctgaa tcagggccgt aaatccggcg ctgaggagaa tcgtgagaac
      301 ccggttgtcc atctgcgcca gcaggtcgac gaaagggggg taatcccgca gcggccgcgt
      361 cgcttcgccc atcatgaaca tgccgaagaa gacgaggccc aggccgaaga ccatcgtccc
      421 gtagcgcttg accagatcgt tgtcgaagaa cgcctgcatg acgaacccca ccaccacggc
      481 gaccagggca tactcggtga tcttgaaggc aatgatctga gcggtgatcg tggtgccgat
      541 ctccgcgccc atgatgacgc caatggactg ctgcagcgtc atcaggcccg ccgacacgaa
      601 cccgacgacg agcaccgtgg tgaccgacga cgattggatc acggccgtgg tcacaccgcc
      661 ggccagaacc cccttgaacc ggttcgtcgt catcttggcc agcaggtcac ccatgcgcgc
      721 accggcggtg accttgagca tgttggtgag ctgatcgagc ccaaacagaa aaatcgccag
      781 cccgccgaga aggccgagaa gcatggttcc caggtcgatg gcgtcacccc ccatgctcgc
      841 tatctcaccc gactcatcgt gaccgccccg tcacgttggc cgcgagtgcc caggcgctga
      901 aggagcgctc agttcggcaa ttcgaagaca tacaacgcct ggccgttggc aggctggtag
      961 atgtcggggc tgatgaccgc ggtgagcgcc ctgaaaacgc ccatcccggt aggcaccgcc
     1021 agatactgct tcccgcccgc acgataggtg acgggatacc cgtgtgtggg cgcgccgagc
     1081 cgtgcctgcc acagaaggcg cccgtcggcg acgtcgaatg ccttgacgta acggtccagg
     1141 tcaccgatga acacgagacc acctgccgtg gtcagggccc cggtcatgaa catggcccgc
     1201 tgctcgtgaa cccaacgctc ctccagggtg gcagcatcca ccgcaaccag gcggcccagg
     1261 ttgccattac tgccaggcat ctcgtaggag cgtgaatcac ccccgaaccc gccttctccc
     1321 accttcgact ccacggggcg cccgaccatg tcggcacaga gccgatgcag cggaatgacg
     1381 aggcttcgcg cctgggggct gtaagccatc gcctgccagt tgtgaccgcc gtagataccc
     1441 ggacaggcgc tgaacgtctc cccgacgcca gcggaccgga tgtcctcgcg atagcgcacc
     1501 cgaccggtct cgggatcgac gtgctcgaag atgttctgct cgatggtttc cacatgagcc
     1561 acgtactgcc ccgtcgcccg atcgagtttc cagagaatgc cgtccttgcc gacggtaaac
     1621 agcagccgtg cgccttcgac gtccaccagc acccgctcga accccacctc catgtcgatg
     1681 gtttcacccg gaacgtgctg gaagtaccag acgatcttcc cggttcgcgg atccatggcc
     1741 agcgtggagt tggtgtacaa cgccgcgtcg tccaccgtca tgccccgact cgcagctacc
     1801 cagggcttgg cctgggacgt tccgatgaaa aagagtttca gcttcggatc gtagctgccg
     1861 gcaatccagg tgtcgccgcc tgctctgaac gacggctcga gtccgcccca gctggcgtcg
     1921 ttgacgtccc cgggcaatgc gatggtggac gtccgccaca gctcacgacc cgtgtccgca
     1981 tcatggccgg taacaaaaca tccatcctcc ttgtaacgct cgcacccgtt gatgccgctg
     2041 agaaccacgc catcgccgac gatgggcccg ctggtatgtg tatagccttg tctgtaatcg
     2101 gctttgaccg cgcgccagac ctgctctccg gtacgggcat ccacagccac gagagcggcg
     2161 tcgtaggtcg ccatgaagag cttgtcacca tagatggcaa tgttacgcag gggaccgcca
     2221 agagtacgcg cggcctcggg atactcgtag gcgtactccc agatcacatc tcccgacgag
     2281 ccatcgacgg cctggatcag gttgcgggca tgggtgaggt acatcacccc gtcgtggacg
     2341 aggggcgtga cctggttgct gccgtcgcgc atggcgagca cccaggcgag tttcagcttg
     2401 ccgacggtct cccgattgat gtcgtccagc gggctgtagc cctgcccgtc cagcgttcgg
     2461 cgccagctga gccagtcgcc cgctggcggg ctggcgagca tggcgtcggt gacgggaacg
     2521 tacttctgca ccttccggtt gacgaaagca gaccggctgc gggcgatggc gtcgatgctg
     2581 gaggcctcgc cccacgagtc gagcgcctcg cccgaatccg gctcggggtc cgcgccgccg
     2641 cctatccggc gcggcgtcga gaagtcgaag ggcgacgtcg cgtccagcca accgttcgct
     2701 tccagcagat acgtaacgat ggccacgtgc tcttcgtcac gc
//
LOCUS       DBNHQR010000211         3326 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_147470, whole genome shotgun sequence.
ACCESSION   DBNHQR010000211 DBNHQR010000000
VERSION     DBNHQR010000211.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_147470"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /gene="efp"
                     /locus_tag="AC16VR_04820"
     CDS             <1..506
                     /gene="efp"
                     /locus_tag="AC16VR_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIR6010510.1"
                     /translation="CSILENEFVKPGKGQAFNRVKFRNLITGRVWERTFKSGDSLEAA
                     DVMELDMEYLYSDGDFWHFMRTDGSYEQSAAPPSAVGDAKDWIKEQEVCQVTLWNDEP
                     ISVTPPNFVELQVTETDPGLKGDTAQGGTKPATLSTGATIKVPLFINVGDVLRVDTRK
                     GEYVSRA"
     gene            503..1378
                     /locus_tag="AC16VR_04825"
     CDS             503..1378
                     /locus_tag="AC16VR_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase GenX"
                     /protein_id="HIR6010511.1"
                     /translation="MNAPPPAWRPACSIEALGARAAMLRTIRDFFFERGVIEVQSACL
                     GAHTVTDPAVDSLAVPGIGYLQTSPEYQLKRLLAAGAPSLYQLGPVFRGGEAGRLHNP
                     EFTMLEWYRLGYTDAELMAEVAELIDSVLGAADYERLSYERVVALGGGAESGAERAEL
                     DLAFADGIAALAGRRVFVVDYPADQAILARLRPGRPNVAARFELCVDGVELANGYWEL
                     GDRAAIERRFAEDLAARRQAGSELPEPDGAFLDAMDAGLPECAGVALGVDRLLMIKLG
                     AGSLAEVMAFPADRA"
     gene            complement(1379..2206)
                     /gene="asd"
                     /locus_tag="AC16VR_04830"
     CDS             complement(1379..2206)
                     /gene="asd"
                     /locus_tag="AC16VR_04830"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937125.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HIR6010512.1"
                     /translation="MSAWFAALQRLLPQHELSRFVGALAKSEAPWIRKPFIHGFARAY
                     GVSLDEAVCQELDDFRCFNDFFTRALRQDARPLARDPAAVLCPADGTVSQTGLIRDGQ
                     LLQAKGHRYSLRSLTSEADRAFEGGTFATVYLAPSDYHRVHLPADGTLLATTAIPGAL
                     FSVNAQTETTVEGLFARNERLVCRFRTGHGQMLVVLVGALIVASIETVWAGPESPYQS
                     VTTTSYDDAFARGAEIGRFVLGSTVIVCFEPGRIELLPHIKAGATVRMGEAIGAFCD"
     gene            complement(2203..2946)
                     /locus_tag="AC16VR_04835"
     CDS             complement(2203..2946)
                     /locus_tag="AC16VR_04835"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000098 - sulfur amino acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HIR6010513.1"
                     /translation="MVHVGDAAGYLQGHVPGAVLVEPGELIGGAPPATGRLPSRERLE
                     ALFSRIGFDPDQEVIVYDDEGGGWAGRMAWTLDVVGHTRWTYLNGGIHSWLAAGLPVE
                     TGPGLPVPPANVTIDIQGEPIAELEDVMAAIEDDDQVVWDVRSREEYLGLRSGSRRAG
                     HVPGAVNVDWMHLKDPERDLRLVDDVRSLLESRGIVPGKRIITHCQTHHRSGLSYMVA
                     RLLGYPRICAYHGSWAEWGNRDDTPVSRS"
     gene            3132..>3326
                     /locus_tag="AC16VR_04840"
     CDS             3132..>3326
                     /locus_tag="AC16VR_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009613492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HIR6010514.1"
                     /translation="MAMPTNLVWMDLEMTGLDPETDVIIEMATVITDSDLNILAEGPV
                     LAVWQPQAKLDAMDEWNQTHH"
BASE COUNT          550 a         1092 c         1143 g          541 t
ORIGIN      
        1 cctgctcgat cctcgagaac gagttcgtga agccggggaa gggccaggcc ttcaatcggg
       61 tcaagtttcg caatctgatc accggtcggg tttgggagcg gacgttcaag tcgggcgaca
      121 gcctggaagc cgcggacgtc atggagctcg acatggagta cctgtactcg gacggcgact
      181 tctggcactt catgcgcacc gacggcagct acgagcagtc ggcggcgccg ccgagcgcgg
      241 tgggtgacgc caaggactgg atcaaggagc aggaggtctg ccaggtgacg ctctggaacg
      301 acgagccgat ctccgtcacg ccgccgaact tcgtcgagct tcaggtgacc gaaaccgacc
      361 cggggctcaa gggtgatacc gcccagggcg gcaccaagcc ggccacgctg tctaccggcg
      421 cgaccatcaa ggtgcccttg ttcatcaacg ttggagacgt gctgcgcgtc gatacccgca
      481 aaggcgagta cgtcagccgc gcttgaacgc gccacccccc gcctggcgcc cggcgtgctc
      541 gattgaagcg ctcggcgcgc gcgccgccat gctgcggacg atccgggatt tctttttcga
      601 gcgcggggtc attgaggtgc agtctgcctg cctcggcgcg cacaccgtca cggacccggc
      661 cgtcgactcg ctggcggtgc ccgggatcgg ctatctgcag acatctcccg agtatcagct
      721 caagcgcctg ctcgcagccg gggcaccttc cctttaccag ctcgggcccg tatttcgcgg
      781 cggggaggcg ggacggttgc acaaccccga attcacaatg ctggagtggt accggcttgg
      841 ctataccgac gcggagctca tggccgaggt tgccgagctg atcgactcgg tgctgggggc
      901 cgccgactac gagaggctca gctacgaacg ggtcgttgcc ctgggcggtg gcgccgagag
      961 tggggcggag cgtgcagagc tggacctcgc gtttgcggat gggatcgccg ccctcgccgg
     1021 ccgccgggtg ttcgtggtgg actatccggc agatcaggca atcctcgcgc ggctgcgccc
     1081 cgggcggccg aatgttgccg cgcgtttcga gctgtgcgtc gacggcgtgg agcttgcaaa
     1141 cggctattgg gagctcggcg atcgtgccgc catcgagcgc cgcttcgccg aggatctcgc
     1201 cgcgcgcagg caagcgggtt ccgagctgcc tgagccggac ggcgcgttcc tggacgctat
     1261 ggacgcgggg ctgcccgaat gcgccggcgt ggcgctgggc gttgatcggt tgctgatgat
     1321 caagctgggt gcggggagcc tggcagaggt catggccttt cccgccgacc gggcctgatc
     1381 agtcgcagaa agcgcctatc gcttcgccca tgcgaacggt cgcgccggcc ttgatgtgtg
     1441 gcagcagctc gatcctgcca ggctcgaagc agacgatcac cgtggagccc agcacaaagc
     1501 ggccgatctc cgcgccccgt gcgaaggcgt catcgtagga ggtcgtcgtg acgctctggt
     1561 agggcgactc ggggcccgcc cacaccgttt cgatgcttgc aacgatgagc gcgcccacca
     1621 gaacgacgag catctggccg tggcccgtgc ggaatcggca gaccagccgc tcgttgcgcg
     1681 cgaacaggcc ctcgacggtg gtctccgtct gcgcgttgac ggaaaagagc gcccccggaa
     1741 tcgccgtggt ggcgagcagg gtcccgtccg ccggcaggtg gacgcggtga tagtcgcttg
     1801 gtgcaagata gacggttgcg aacgttccgc cttcgaatgc ccgatcagcc tcgctcgtca
     1861 gcgagcgaag cgagtaccgg tgccctttcg cctgcagcag ctgaccgtcc cggatcaggc
     1921 ccgtctggct cacggtcccg tcagccgggc agagcacggc ggccggatcc cgggccagcg
     1981 gccgggcgtc ttgacgtagc gcgcgggtga agaagtcgtt gaagcagcgg aaatcgtcca
     2041 gctcctgaca taccgcctcg tcgagcgaca ccccgtaggc tcgtgcgaac ccgtgaatga
     2101 agggtttgcg gatccagggc gcttctgatt tcgccagggc gccgacgaac cgggacagct
     2161 cgtgctgggg aagcagcctc tgcagcgcgg cgaaccaggc gctcacgacc ggctcaccgg
     2221 ggtgtcgtcg cgattgcccc actcggccca ggatccgtgg taggcgcaga ttcgggggta
     2281 gccgagcagc cgcgcgacca tgtaggagag gccggagcgg tgatgagtct ggcagtgggt
     2341 gatgatgcgc ttgcccggca cgattcccct ggattcgagc agggagcgaa cgtcgtccac
     2401 caggcgcagg tcgcgctcgg ggtccttgag gtgcatccag tcgacgttca ccgcgccggg
     2461 aacgtggccg gcccgacggg aaccgctgcg cagccccaga tactcctcgc gagagcggac
     2521 gtcccagacc acctgatcgt cgtcctcgat ggcagccatg acgtcctcga gctccgcgat
     2581 gggttcgcct tgaatgtcga tcgtcacgtt tgctggcggc acgggcagcc ccggtccggt
     2641 ctcaaccggc agcccggcgg ccagccagct gtggatgccg ccgttcaagt aggtccagcg
     2701 ggtgtggccc accacatcca gcgtccacgc catccggccg gcccagccgc cgccctcgtc
     2761 gtcgtagacg atgacctcct ggtccggatc gaaaccgatt cgcgagaaaa gcgcttccag
     2821 cctctccctg ctcggcagcc ggccggtagc cggcggcgcg ccgccaatca gctccccagg
     2881 ctcgacgaga acggcgccgg gaacgtgccc ctgcaggtag ccggccgcat cgcccacatg
     2941 caccacgagc cggccctccg ggtcggcgcc ggccagctcc tcgggttcga tgattctcgg
     3001 tggcgcgatg acggcttcct gtccggggca aaagggccgc ctatcttagc cgagaactga
     3061 atcgccgtgg cagatcccag gcttgtggca gaatggccgg cggcgggcgg gcgacccgca
     3121 tccgtttagg agtggcaatg ccgacaaacc tggtgtggat ggatctcgag atgacgggtc
     3181 tcgaccctga aaccgacgtc atcatcgaaa tggccacggt gatcaccgac agcgatctca
     3241 acattctcgc cgagggcccg gtgctcgccg tttggcaacc ccaggcaaag ctggacgcca
     3301 tggacgagtg gaaccagacc caccac
//
LOCUS       DBNHQR010000212         3990 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_337641, whole genome shotgun sequence.
ACCESSION   DBNHQR010000212 DBNHQR010000000
VERSION     DBNHQR010000212.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3990)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_337641"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            334..927
                     /locus_tag="AC16VR_04845"
     CDS             334..927
                     /locus_tag="AC16VR_04845"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR6010515.1"
                     /translation="MELILVRHGLPVREELETGRADPPLSETGRAQAERVALWLADAP
                     VDAVYSSTMQRARQTAQPYAAAIGLEVVEREGIAEFDRHESSYVPLEELKAEDPEAWR
                     AFVAGGYNEQVDIHQFHAEVVNSLEQLIARHSGQRIAVFCHGGVINVWTAHVIGITPR
                     LFFEPAYTSIHRFLCARSGERNLVSLNETGHLRAPIT"
     gene            1162..1935
                     /locus_tag="AC16VR_04850"
     CDS             1162..1935
                     /locus_tag="AC16VR_04850"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR6010516.1"
                     /translation="MSAVVNVRSKKVPTLYEADESKYAGPHRWQIRAWARVARRLWRY
                     RAYTRWARQAFSRIEVSGAEHLDSVEGPCVFIANHQSHLDTILVHAVLPEKIRSKIYF
                     GAAQDRWFVKGKRKLVLKPWYQSLALGNFPILRGGGTRALAYASWLLDRGQHVFLFPE
                     GTRATSTEVGEFKHGAAILALEAGVPVVPVYIAGAQKIRPKGTREVARGVGGIEILEP
                     VRFSAGSDVAAATRLLQDRLGEVHARHRGGDRERLPSAA"
     gene            complement(1940..2134)
                     /locus_tag="AC16VR_04855"
     CDS             complement(1940..2134)
                     /locus_tag="AC16VR_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017738733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPXCG motif-containing cysteine-rich protein"
                     /protein_id="HIR6010517.1"
                     /translation="MTHYEVRSVSLSCPYCGETIEILIDLSLSEQEYIEDCSVCCRPI
                     LLDVSVTDDEPLVTARSENE"
     gene            complement(2131..3096)
                     /locus_tag="AC16VR_04860"
     CDS             complement(2131..3096)
                     /locus_tag="AC16VR_04860"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR6010518.1"
                     /translation="MITIPAFIAATVAVTAALPVLAIAAAICSVVPATKGALPTLGFI
                     AGYLWCETIGIVTAFAIWIRHREADRFLQANFRLQCWWANALKVIAERLFRLTFDVEG
                     EECLSGPAAIMLPRHASIADTIIPMVYYAIPRQIRLRYVLKRELLADPCLDIVGHRLP
                     NYFVDRHGQDSERARRGVAALLADIGDDEGALIYPEGTRFSEAKRSQLLERYAASEDM
                     LAQLRRWTELLPPRLGGTLALLDANPGRDLIFCAHTGFEGSSHFATLINGSWIGAHIR
                     ISFWRVPGQAVPAETDAQREFLFDQWDRMQAEVLRLQAAPGGTAP"
     gene            complement(3101..>3990)
                     /locus_tag="AC16VR_04865"
     CDS             complement(3101..>3990)
                     /locus_tag="AC16VR_04865"
                     /inference="COORDINATES: protein motif:HMM:NF013703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR6010519.1"
                     /translation="MAAGNIMRASGSANVPGLVMTLGAVVNLALDPIFIFGWFGFPRL
                     ELAGAAVAGAISRVGTAAILLYFVARGGLLLTTGWKPRFLNSCHRILEVGLPAMATQL
                     IGPVSSAVITRLLAGHGEIVVAGFGVATRVEAVAVMLLFALSGSIGPFVGQNWGAGRS
                     ERVREGLRFTYLFCLAWGLAAAILMAFFGSTVASWVDSEPGVIGTAAFYLAVVPWSYG
                     LWGVLMMSSASFNALGKPIPSTILSFTRMFIMYIPLAVLLEGQFGYTGIFVATAATNG
                     VMGILAFLWFRSAFFADAR"
BASE COUNT          765 a         1267 c         1289 g          669 t
ORIGIN      
        1 gatcgcagtg tgtctgatgc gcaccagctc gccggtcggg tcgggaagct cttccgactc
       61 ccagctgaga acctcgggac cacctgcttc gtggatgcga tagacgccga ccatgctccc
      121 tccccaatcg agtcatgaac cgcaagtctc ggccatggcg cctggaggtg caatcgccct
      181 gcaatgaccc gacgagccct gccgtgccgc tggtcggtga gcctcggcta tacttcgacg
      241 cgggtcgctc ggcccgcccc gcggccgatc cggcacctcc agcttccggg cggtccgccg
      301 tcgcaggccg aagacaccac agaggacctc atcatggagt tgatactcgt tcgtcacgga
      361 ttacccgttc gcgaggaact ggagaccgga cgtgcggacc cgccgctttc agagaccggc
      421 agggcgcagg ctgagcgcgt cgcgctgtgg ctggccgatg cgcccgtcga tgccgtctac
      481 tcgagcacca tgcaaagggc gcggcagacg gcacagccct acgcggccgc tatcgggctg
      541 gaggtcgtcg agcgggaggg aatcgccgag ttcgaccgcc acgagtccag ctacgtgccc
      601 ctcgaggagc tcaaagctga ggatccggaa gcgtggcggg cgttcgtcgc cggcggctat
      661 aacgagcagg tggacattca ccagttccac gccgaggtgg tgaactccct ggaacagctc
      721 atcgcccgcc actccggtca gcgaattgcc gtgttctgcc acggcggcgt gatcaatgtg
      781 tggacggcgc acgtcatcgg gatcacgcct cgtctgttct tcgagccggc ttacacgagt
      841 atccaccggt tcctgtgcgc tcgttccggt gagcgcaatc tggtcagcct caacgagacc
      901 gggcacctgc gcgcgccgat tacctgacct cccatccgac cggccgcgtc tcgctttgca
      961 tcgaagtggt gcgcggccga acccagtggt cccaagttgg tgcgccggtt acccgccgat
     1021 gccgggcgaa ggccgaaata cggtgccttc actttggcct gcaaattgca atctccatgg
     1081 ggtaacggtc ctgtcatgga cgcgtcctgg agtgcaacgc tccttcgggc gaggtcatca
     1141 acaaagcatt ggaggtcagt gatgtcggcg gtagtaaacg tcaggagcaa aaaggtcccc
     1201 accctgtacg aggcggacga gtcgaagtac gccgggccgc accggtggca gattcgggcg
     1261 tgggcacggg tggctcggcg gctctggcgc taccgggcat acacccgttg ggcacggcag
     1321 gcgttcagcc gcatcgaagt ctcgggtgcg gagcacctcg actcggttga ggggccttgc
     1381 gtcttcattg ccaatcacca gagccatctc gacaccatac tggtacacgc cgtgctgccg
     1441 gagaagatcc gttcgaaaat ctacttcggc gcagctcagg atagatggtt cgtcaaaggt
     1501 aagaggaagc tcgtactcaa gccctggtat cagtcgctgg ctctcggaaa ctttcccatc
     1561 ctccgtggcg gcggcaccag agcgctggcg tacgccagct ggctgctcga ccgtgggcag
     1621 cacgtgttcc tctttcccga gggcacccgt gcaacctcga cggaggtcgg ggagttcaag
     1681 cacggcgcgg ccatcctcgc gctcgaagca ggcgttcccg tcgtgcccgt ctacatcgcg
     1741 ggagcgcaga aaatcaggcc caaaggcacg cgggaggttg cccgaggggt gggtggaatc
     1801 gagattctgg aaccggtgag gttctctgcg gggagcgacg tagccgccgc gacccggctt
     1861 ctgcaggacc gtctgggtga ggttcatgcc cggcacaggg gtggtgatcg ggagaggctg
     1921 cctagcgccg cctagcgcgt cactcgttct cgctgcgcgc ggtcaccagg ggctcgtcgt
     1981 cggtcacgga gacgtccagc aggatgggcc ggcagcaaac ggagcagtct tcgatgtatt
     2041 cctgctccga caggctgagg tcgatcagaa tctcgatggt ctcgccgcaa tacggacagc
     2101 tcagggagac ggagcgaacc tcgtagtggg tcacggggcc gttcctcccg gagcggcctg
     2161 cagacgaagc acctcagcct gcatcctgtc ccattggtcg aacaggaatt cgcgctgggc
     2221 atcggtctcc gccggcacgg cctggcctgg cacgcgccag aaggagatgc ggatgtgggc
     2281 gccgatccag gatccattga tgagcgtggc gaagtggctc gacccctcga agcccgtgtg
     2341 cgcgcagaaa atcagatccc gccccggatt ggcatcgagc agcgcaaggg ttccgcccag
     2401 acgcgggggc agcagttcgg tccagcgtct aagctgggcg agcatgtcct ccgaagccgc
     2461 gtagcgctcg agcaactgcg atcgttttgc ttcggagaac cgggtgcctt ccgggtagat
     2521 cagcgcgccc tcatcatcac cgatgtcggc gagcaacgcg gccacacctc gtcgggcccg
     2581 ctcgctgtcc tggccgtgcc gatcgacgaa gtagttgggt agccggtggc cgactatgtc
     2641 caggcagggg tcagccagca gctcccgttt cagcacgtac ctgagcctga tttgccgggg
     2701 aattgcgtag tagaccatgg ggatgatcgt gtcggcgatg ctggcgtgtc gcggcagcat
     2761 gattgccgcc ggacccgaaa gacactcttc gccctcgaca tcgaaggtca gcctgaagag
     2821 cctctccgcg atgaccttca gcgcgttggc ccaccagcat tggagcctga agttcgcctg
     2881 gaggaatcgg tctgcctccc gatgccggat ccagatcgcg aatgccgtga cgatcccgat
     2941 ggtctcgcac cacaggtagc cggcgatgaa tccaagcgtc ggcagcgcgc ctttggtagc
     3001 tggaacgacg ctgcaaatgg ccgccgctat cgccagcacg ggaagcgccg cggtaacggc
     3061 caccgtggcc gcaatgaatg ccggaatcgt gatcaagcgc ctaacgagca tctgcaaaaa
     3121 acgcgcttcg gaaccagagg aacgccagta tgcccatgac gccgttggtt gctgcggtcg
     3181 cgacgaatat tcccgtatag ccgaactgcc cttccagcag cacggccagc ggaatgtaca
     3241 tgatgaacat ccgcgtaaac gagaggatgg tggagggtat cggcttgccc agcgcgttga
     3301 acgacgccga tgacatcatc agaacgcccc agagaccgta gctccagggg accaccgcaa
     3361 gataaaacgc cgcggttccg atcacaccgg gctcagagtc tacccatgat gcgacggtgc
     3421 tgccgaagaa cgccatgaga atcgcggctg ccagccccca ggccaggcag aagagatagg
     3481 tgaagcgcag gccttcccgg acacgctcgg accggcctgc gccccagttc tggccaacga
     3541 atggaccaat gctgccggac aaggcgaaga gcaacatgac cgccacggcc tcgacccggg
     3601 tcgcaactcc gaagcccgca accacgatct ccccatgacc ggccaggagt cgagtgatca
     3661 cggccgagga aaccggtccg atgagctggg tggccatggc gggaaggccg acctccagaa
     3721 tccgatggca cgagttgaga aatctgggct tccacccggt tgtcagcaac aggccgcccc
     3781 tggcaacgaa atagagcaga atcgcggccg tgcctactct ggagatggct ccggccacgg
     3841 ccgcgcctgc aagttccagc cgtggaaacc cgaaccagcc gaagatgaaa atcggatcga
     3901 gcgcaaggtt gaccaccgcg ccaagggtca tcaccaggcc gggcacgttg gcgcttccgc
     3961 tggcgcgcat gatgttgccg gcggccatgg
//
LOCUS       DBNHQR010000213         9321 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1098058, whole genome shotgun sequence.
ACCESSION   DBNHQR010000213 DBNHQR010000000
VERSION     DBNHQR010000213.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9321)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9321)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9321
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1098058"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="AC16VR_04870"
     CDS             <1..440
                     /locus_tag="AC16VR_04870"
                     /inference="COORDINATES: protein motif:HMM:NF039797.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6491 family protein"
                     /protein_id="HIR6010520.1"
                     /translation="ALLTGCASDPAETAAAQQEVSEQIDTILTQPLGEEDYGGGERCI
                     STNLYDSVDVLDTRHVLFKGRGNRLWVNTLRSTCVGLRKRDVLQFEIRGSQICNLDTF
                     TAVSNSFGWQRTSATCSLGDFSPVTAEQVAMIEAALDTKKKKK"
     gene            524..937
                     /locus_tag="AC16VR_04875"
     CDS             524..937
                     /locus_tag="AC16VR_04875"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR6010521.1"
                     /translation="MSEPVRSGDPFDLFSRMMGGTDREPHAGSRDWLPAVDIREEQEG
                     YLLVMDVPGLGAEDLDVDLHEGVLTVRGARSAEETKQGEHYVRVERRHGKFARQFRVP
                     PSARPEDLRANVKDGVLTIHVPKGGAPETRRVPVS"
     gene            947..1423
                     /locus_tag="AC16VR_04880"
     CDS             947..1423
                     /locus_tag="AC16VR_04880"
                     /inference="COORDINATES: protein motif:HMM:NF037143.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR6010522.1"
                     /translation="MDLNQIKADVGQTWKWLADGWRSLSDKAVNALTYFTPGRNEPAD
                     PDEIRWGLLAADVSESGEHVTVELEAPGLEKDEIDVSVDERQVTITGQKRVESERREG
                     AMVVRERAFGRFQRTIPMPVAVTADGAEASYRRGVLRIRVPKVASPGGRSIKIEKG"
     gene            complement(1404..1928)
                     /locus_tag="AC16VR_04885"
     CDS             complement(1404..1928)
                     /locus_tag="AC16VR_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HIR6010523.1"
                     /translation="MVENIIQLIEEVAEALLENRSRLITAESCTGGMVAAELTSRPGS
                     SEWFEGALVTYRLSAKQRLLGVPAAMLDRYGPVSEPVARAMAEGALRVSDADVSVAIT
                     GIAGPDGGDMLAPVGTVWFAWARRDEPGAEPVCLQATQHRFSGSRDEVRTQAVALALE
                     GVLASLSGINPSRS"
     gene            2000..4438
                     /locus_tag="AC16VR_04890"
     CDS             2000..4438
                     /locus_tag="AC16VR_04890"
                     /inference="COORDINATES: protein motif:HMM:NF015155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6010524.1"
                     /translation="MTTYLRVLNYPRTLLTLVALLLVVAGVCGTRFSFDASSDTLVVE
                     GDPDLATYLRIAETFGGDEFLLLTFTPRDGDALDPANLATLRALQQEIAALEGVGSVF
                     SILDAPLLKSPPVPLAELARGFRTLESADVDRPSAREELKESPVFGGLLITADARTTA
                     MRVDISLDRELLVLDEERARLRSQAERSAAEVKRLAELEVARETLREAYVNGRERLIG
                     QIRDVRDRYADRGVLHLGGVPMIAADMIAFVKSDLVVFGGSVVVIVMVTLYGFFRRLR
                     WVLLPVVTSAVTIGFTVGVLGLAQWPATVISSNFVSLLAIITISLTIHLVVRYRELAH
                     LHPDMAADELVRQTMVSKFAPCLYNALTTIAAFGSLTASQIVPVEDFGWMMCIGIAIG
                     FFTSFTLFPALLLLLGRVAGPSLLSRELGVTRVFSHLARWRAPLVVVISVVWAAFAAL
                     GVSMVSMDNRFLEYFRENTDINQGMAFVDAQLGGTVPFDVVLEFPPFENLVEEEDDFF
                     SEEETYPERYWFTRDKLDKLMLVHRYVEARPEVGKVLSLTSLEDLARQFTEGDALSNL
                     EVAAVLDVLPEHLRDELIRPYASPSTGELRINARVIESGRSFDRQALAADIRDFAVTQ
                     GAVPADAVQVTGMMVLFDSMLKQLLSSQLDTLVYVLLATFAMFVILLRSFLYALLGLI
                     PNVIAAASVIALMGYVGIPLDMMTITIAAISIGIGVDDAIHYLHRFDEERRRREDVRM
                     AVAWSHATIGRAMYFTSVTVIIGFSVLVFSNFVPTIFFGLLTAAAMALALIANLTLLP
                     SLLVLVIGRREASA"
     gene            complement(4530..6014)
                     /gene="trpE"
                     /locus_tag="AC16VR_04895"
     CDS             complement(4530..6014)
                     /gene="trpE"
                     /locus_tag="AC16VR_04895"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014851564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HIR6010525.1"
                     /translation="MNQAQFAELAEAGYNRIPVVFETLADLDTPLSTYLKLARGPYSY
                     LLESAHGGEKWGRYSIIGLPCQTVLKVQGNQITVETDGELVETHESPDPLSFIEEFQA
                     RYRVAALPELPRFYGGLVGYFGYDTVRYAERRLADGAPPDPLGTPDIVLMVSEDLVVF
                     DNLRGRMQLISLVDPAEPDVYQRTMERLRDRALALSEPVPPIPESATNQPVLESDFAS
                     EFGEEPFKEAVEKIREYIRAGDVMQVVLAQRMSLPFGADAIDLYRALRSLNPSPYMYF
                     MDLEEFQIVSSSPEILARLEDGKIINRPLAGTRRRGHTPEEDLALEQELLSDPKELAE
                     HLMLIDLGRNDVGRVSEIGSVEVTEQFVIERYSHVMHISSNVVGRLAEGKTAIDVLRA
                     TLPVGTLSGAPKIRAMEIIDELEPVKRGIYGGAVGYLAWNGNMDTAIAIRTAVIKDGT
                     LYIEAGGGVVADSVPRLEWKESMNKGRAIFRAAAMAENRFRDLG"
     gene            complement(6025..6669)
                     /gene="gph"
                     /locus_tag="AC16VR_04900"
     CDS             complement(6025..6669)
                     /gene="gph"
                     /locus_tag="AC16VR_04900"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01449.2"
                     /note="PGP is an essential enzyme in the glycolate salvage
                     pathway in higher organisms (photorespiration in plants).
                     Phosphoglycolate results from the oxidase activity of
                     RubisCO in the Calvin cycle when concentrations of carbon
                     dioxide are low relative to oxygen. This enzyme is a
                     member of the Haloacid Dehalogenase (HAD) superfamily of
                     aspartate-nucleophile hydrolase enzymes (PF00702); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HIR6010526.1"
                     /translation="MKPYTGYLFDLDGTLIDTAPDINAALNHALTQCGAPTVNEALTR
                     HWVGHGARVLVEQALAYHELPEEDLAPIHEAFLAYYGDHAAEHSRVYPGVVDTLTALK
                     ERGARLAVLTNKLTRFSLPILSTLGIDGYFDATICGDTAAAPKPAADPALLACRELRT
                     TPGDSLVVGDSQTDVLCARAAGCPVVCVRDGYNHGVPAEQLGADAVIESFLDLL"
     gene            complement(6666..7358)
                     /gene="rpe"
                     /locus_tag="AC16VR_04905"
     CDS             complement(6666..7358)
                     /gene="rpe"
                     /locus_tag="AC16VR_04905"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013981691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIR6010527.1"
                     /translation="MSYWIAPSILSADFSRLGAEIEAVLGAGADIIHFDVMDNHYVPN
                     LTIGPLVLQSLRKAGIDCTMDVHLMVKPVDRLVGDFIEAGADIISVHPEATEHLHRTL
                     SLIRDGGARAGLVFNPATPLGVLEEVLDLVDLVLVMSVNPGFGGQAFIASSLDKLAAA
                     RRLIDGSDRAIRLEIDGGIKADNIALAAQAGADTFVAGSAIFGSPDYAATIASMRAAL
                     EGVQPGTPRGAA"
     gene            complement(7370..8041)
                     /locus_tag="AC16VR_04910"
     CDS             complement(7370..8041)
                     /locus_tag="AC16VR_04910"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR6010528.1"
                     /translation="MRAMILAAGRGERLKPLTDAVPKPMLTVAGEPLIARQLRALAAA
                     GITRVVINLHHLGEQIASFCGSGKDYGVAIDYSREDELLDTGGGIVKALPLLGDAPFL
                     ILNGDILTDFPFQTLATALPDWADLHLVLTPRPAHRTHGDFDWEAGRVTGRGESYVYC
                     GIAVLRPELFDGRIIEPFSLQQILFDAVAGGRVSAEIWTGTWIDIGSHDQLRAANEHL
                     KSEPG"
     gene            complement(8038..9045)
                     /locus_tag="AC16VR_04915"
     CDS             complement(8038..9045)
                     /locus_tag="AC16VR_04915"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HIR6010529.1"
                     /translation="MESRSIPTELAQWLAGELARLDLDPTFTSQALLVEASYRCFYRI
                     RSRHRGGEALSLVAMESPPERENNQQFVTLAKVFAAHGVGVPRILAADLTDGFLLMTD
                     LGERHFADAYQSGERDRALAAGIDTLIRLQTVDDPAVPLYTARRFRDELGIFREWFVE
                     RWLGEPFPAEDLAGIPERLVACVEQQLQVCVHRDFHSRNLLLAADGSVGVVDFQDALI
                     GPVSYDLASLLRDCYYRFPEDEIARWRDYYLERSPHALDPEEFARDLDLTAAQRQLKA
                     VGIFARLDLRDGKTSHLRHIPPVLDHLAALSARHDTLTPLGPHLAAWQKAAERRLQAR
                     R"
     gene            9109..>9321
                     /locus_tag="AC16VR_04920"
     CDS             9109..>9321
                     /locus_tag="AC16VR_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010530.1"
                     /translation="MTSRPSSRRRAGCCLLLAGLLTGAAAAEDAAPVTGVAERYWTPR
                     DELPERVAARLPSYCDGAYLQPAFPYP"
BASE COUNT         1500 a         3025 c         3172 g         1624 t
ORIGIN      
        1 gcgcgcttct taccgggtgc gccagcgatc cggcggagac ggcggcggcg cagcaggagg
       61 tttccgagca gatcgacacg atcctgaccc agccgctcgg cgaggaagac tatggcggtg
      121 gagaacgctg catctccacg aacctctacg actcggtaga tgtcctggat acccggcacg
      181 tgctcttcaa gggtcgcggc aaccgcctgt gggtcaacac gctgcgcagc acgtgcgtcg
      241 gcctgcgcaa gcgcgacgtg ctgcagttcg aaatccgcgg cagccagatc tgcaacctcg
      301 acacgttcac ggcggtgagc aattccttcg gatggcagcg aacgagcgct acctgctctc
      361 tgggtgactt ctctcccgtg accgctgagc aggtcgccat gatcgaagcc gctctggata
      421 ccaagaaaaa gaaaaagtga ccggctgctg cgggaagtac ttagtgaaat tcggcccgct
      481 gggggcacca ttacggctct agaccaactt ggagccagca gccatgagcg agcccgtacg
      541 atcgggagat cccttcgacc tgttcagtcg gatgatgggc gggacggacc gtgaacctca
      601 cgcggggagc cgggattggc tgccggccgt ggatatccgg gaggagcagg agggatatct
      661 tctggtcatg gatgttcccg gccttggggc cgaggacctg gacgtcgacc tgcacgaggg
      721 cgtcctcacg gtgcggggcg cccggtcggc cgaggagacc aagcagggcg agcactacgt
      781 tcgcgtagag cgccgtcatg gtaagttcgc ccgccagttc cgcgttccgc cgagcgcgag
      841 gccggaggat ctgcgcgcga acgtcaagga cggtgtgctc accattcacg ttcccaaagg
      901 cggcgcaccg gagacccggc gcgtgcccgt cagctaggag cccgccatgg acctcaatca
      961 gatcaaggcc gatgtggggc agacctggaa gtggctggca gacggttggc gcagcctgag
     1021 cgacaaggcc gtcaacgcgc tcacatactt tacgccgggg cgcaacgagc cggccgaccc
     1081 cgatgagatc cgctgggggc tcctggccgc cgatgtgtcg gaaagcggcg agcacgtgac
     1141 cgtcgagctc gaagcgccgg ggcttgagaa ggacgagatc gacgtgtccg tggacgagcg
     1201 gcaggtgacg atcacgggtc agaagagagt cgaatccgag cggcgtgaag gagccatggt
     1261 ggttcgggag cgcgcgttcg gccgatttca gcggacgatc cccatgccag tggcagtcac
     1321 cgccgatggc gccgaggcga gctaccggcg cggtgtgctc aggatccggg tgcccaaggt
     1381 ggcaagcccg ggcggccgaa gcatcaagat cgagaagggt tgatcccgct caggctcgcg
     1441 agcacgccct ccagggccag ggcgacggcc tgagttcgaa cctcgtctcg agatcccgag
     1501 aagcgatgct gagtcgcctg caggcagacg ggctcggcgc cgggctcgtc gcgcctggcc
     1561 caggcaaacc aaaccgtgcc cactggggcc agcatgtcgc caccgtcggg accggcaatg
     1621 cccgttatcg cgacgctcac gtccgcatcg ctgacgcgca gcgcgccctc tgccatcgcc
     1681 cgggcgacgg gctcgctgac cgggccgtag cggtcgagca ttgccgccgg cacgccgagc
     1741 aggcgctgct tggcgctcag acgatacgtc accagcgccc cctcgaacca ctccgagctg
     1801 ccgggtcggc tggtgagctc cgctgccacc atgcccccgg tgcaggactc ggcggtgatc
     1861 aggcgcgaac ggttctcgag aagggcctcg gccacttcct ctatgagttg aataatgttt
     1921 tccaccaccg aacaatagcc gaaaattcct tgccgcgagc acgcgtatct ccgatctgca
     1981 caaacacctg caaccgctgg tgacgaccta cctgcgggtt ctgaactatc cgcgcacgct
     2041 gctcaccctg gtggcgctgc tgctggttgt cgcgggcgtt tgcgggacgc gattcagctt
     2101 cgatgcctcc tcggacacgc tggtggtgga gggcgatccg gacctcgcca cctacctgag
     2161 aatcgccgag actttcggcg gcgacgagtt tcttctgctc acgttcacgc ccagggacgg
     2221 tgacgcgctg gatccggcca atctcgccac cctgcgcgcc ttgcagcagg agatagccgc
     2281 tctggagggc gtcggctcgg tcttctccat tctcgatgcg ccgttgctga agagtcctcc
     2341 cgtgccgctg gccgagctgg cccggggttt tcgcacgctg gagtcggcgg acgtggaccg
     2401 gccctcggcc agggaggagc tcaaggagag cccggtgttc ggcgggctcc tcatcacggc
     2461 agatgctcgc accacggcca tgcgggtgga catctcgctg gatcgggagc ttctcgtgct
     2521 cgacgaggaa cgggcccggc tgcgaagcca ggccgagagg tccgcggcgg aggtcaagcg
     2581 gctggcggag ctcgaggttg cccgggagac gttgagggaa gcctacgtca acggccggga
     2641 acgtctgatc ggccagatac gcgatgtgcg cgaccgctac gcggatcgag gcgttctcca
     2701 cctgggcggc gtgccgatga tcgcggcaga catgatcgcc ttcgtgaaga gcgacctcgt
     2761 ggtttttggc ggcagcgtcg tggtgatcgt gatggtcacg ctctacgggt ttttccgacg
     2821 gctgcgctgg gtcctgcttc cggtcgtaac cagcgccgtc accatcggct tcaccgtggg
     2881 cgtcctcgga ctggcccaat ggccggctac cgtcatctct tccaacttcg tctccctgct
     2941 ggcgatcatc accatctcgc tgaccattca tctcgtggtc cgctatcgcg agcttgccca
     3001 cctgcatccc gacatggcgg cggacgagct ggtccggcaa accatggtca gcaagtttgc
     3061 gccgtgcctg tacaacgcgt tgaccaccat tgccgccttc ggatcgctga ccgccagcca
     3121 gatcgtcccg gtggaggatt tcggctggat gatgtgcatc ggcatcgcca tcggtttctt
     3181 cacgagcttc accctcttcc cggcgctgct gcttctgctc ggccgggtcg cggggccgtc
     3241 cctgctgagt cgggagctgg gggttacccg tgtcttcagc cacctggccc gctggcgcgc
     3301 gccgctggtg gtggtcatct ccgtcgtgtg ggcggcgttt gccgccctcg gggtgagcat
     3361 ggtttccatg gacaaccgct tcctcgagta ctttcgagag aacacggaca tcaaccaggg
     3421 gatggccttc gtcgatgccc agctgggcgg cacggtgccc ttcgacgtgg tcctcgagtt
     3481 cccgccgttc gaaaacctgg tcgaggaaga ggacgacttc ttctcggagg aagagaccta
     3541 tcccgagcgg tactggttca cccgggacaa gctggacaag ctgatgctcg tccaccggta
     3601 cgtcgaggcc cgcccggagg tgggaaaggt tctctcgctg acgtcgctgg aggatctggc
     3661 caggcagttc accgagggcg atgcgctttc caacctcgag gtcgccgcgg ttctggacgt
     3721 gctgcccgag cacttgaggg acgagctcat ccggccctac gcgtctccgt ccacgggcga
     3781 gttgcggatc aacgcgcgcg tcatcgagag cgggcggtcg ttcgaccgtc aggccctggc
     3841 cgccgacatc cgcgatttcg ccgtgaccca gggcgcggtg ccagccgatg ccgttcaggt
     3901 gacgggcatg atggttctat tcgacagcat gctcaagcag ctgctcagct ctcagctgga
     3961 tacgctcgtc tacgtgctcc tggccacctt cgccatgttc gtcatccttc tgcgctcctt
     4021 tctctacgcg ttgctcggac tgattcccaa cgtgattgcg gcggccagcg tcatcgcgct
     4081 catgggttac gtgggcatac ccctggacat gatgaccatc accatcgccg ccatcagcat
     4141 cgggatcggc gtggacgacg ccatccacta tctgcaccgc ttcgacgagg agcgccgcag
     4201 gcgggaagac gttcgcatgg cggtggcgtg gagtcacgcc accattggcc gcgccatgta
     4261 cttcaccagc gtgacggtga tcatcggctt ctccgttctc gtgttctcga acttcgttcc
     4321 gaccatcttc ttcgggttgc tgaccgcggc cgccatggcc ctggctctga tcgcgaacct
     4381 gacgcttctg ccgagcctgc tcgtgctggt gatcgggcga cgcgaagcct ccgcgtgacg
     4441 cttcactcgc caacggtggg tcgagtggcg tttgcttcct tgagatggtt cggcttcgcg
     4501 gtaacgaacg gattcacgac gacgtctttc tagcccagat cccggaagcg attctcggcc
     4561 atggcggccg cccggaagat ggcacgcccc ttgttcatgc tttccttcca ttccaggcgt
     4621 ggcaccgagt cggccaccac gccgccgccc gcttcgatgt acagcgtgcc gtccttgatc
     4681 accgcggtgc gaatcgcgat cgccgtatcc atgttgccgt tccatgccag gtaaccaacg
     4741 gcgccgccgt agatgccccg cttcacgggc tccagctcgt cgatgatctc catggcccgg
     4801 atcttcgggg cgccggacag cgtgcccacc ggcagcgtcg cgcgcagcac gtcgatggcc
     4861 gtcttgcctt ccgccagccg tcccaccacg ttgctggaaa tgtgcatgac gtgggaatag
     4921 cgctcgatga cgaactgctc ggtgacctcc acggagccga tctccgagac ccggccgacg
     4981 tcgttgcggc cgaggtcgat gagcatcagg tgctcggcga gctccttggg gtcggacagc
     5041 agctcctgct ccagggccag gtcctcctcg ggcgtgtgcc cgcgccgccg ggtgccggcc
     5101 agggggcggt tgatgatctt gccgtcctcg agccgggcca ggatctccgg tgagctgctg
     5161 acgatctgga attcctcgag gtccatgaag tacatgtagg gcgacgggtt gaggctgcgc
     5221 agcgcccggt acaggtcgat ggcgtcggcg ccgaaaggca gcgacatgcg ctgggcgagc
     5281 accacctgca tgacgtcccc ggcccggatg tactcgcgaa tcttctccac cgcctccttg
     5341 aacggctcct cgccgaactc ggatgcgaag tcgctctcca gcaccggctg gttggtcgcg
     5401 ctttcgggaa tgggcggaac gggctcgctc aatgccaggg cccggtcgcg cagccgctcc
     5461 atggtgcgct ggtagacgtc gggctcggcc gggtcgacga ggctgatcag ctgcatccgg
     5521 ccacgaagat tgtcgaacac caccaggtcc tcggagacca tgagcacgat gtccggggtg
     5581 ccgagagggt cggggggcgc gccgtcggcc agcctccgct ctgcgtagcg cacggtgtcg
     5641 tagccgaaat agcccaccag gccgccgtag aaacgcggca gctccggcag agcggcgacc
     5701 cggtagcggg cctggaattc ctcgatgaag gacagcgggt cggggctttc gtgggtttcc
     5761 accagctcgc cgtccgtctc cacggtgatc tggtttccct gcaccttgag cacggtctgg
     5821 cacggcaggc cgatgatgga gtagcggccc cacttctcgc cgccgtgggc ggattccagc
     5881 aggtaggaat acgggccgcg cgccagcttc agataggtcg acagcggcgt gtccagatcg
     5941 gccagggtct cgaagaccac tggaatccgg ttgtagccgg cctcggccag ctcggcgaat
     6001 tgggcctggt tcacggtctg cccatcaaag gaggtccagg aacgactcga tcaccgcgtc
     6061 ggcgcccagc tgctcagccg ggacgccgtg attgtagccg tcgcgcacgc agaccacagg
     6121 gcatccggcc gccctggcgc agagcacgtc ggtctgcgag tcgccgacca ccaggctgtc
     6181 cccgggagtg gtgcgcagct cgcggcaagc gagcagggcc gggtccgcgg cgggcttggg
     6241 cgccgcggcg gtgtctccgc agatcgtggc gtcgaagtag ccgtcgatgc ccagggtcga
     6301 gaggatgggc aggctgaacc gggtcagctt gttggtcagc acggcgagcc tcgcgccgcg
     6361 ctccttgagc gccgtcaggg tgtcgaccac gcccggatag acccggctgt gctccgctgc
     6421 gtggtcgccg tagtaggcaa gaaaagcctc gtggatgggc gcgaggtctt cctcgggcag
     6481 ctcgtgatag gccagcgcct gctccaccag cacccgggcg ccgtggccga cccagtggcg
     6541 cgtcagggcc tcgttcaccg tgggcgcacc acattgcgtc agagcatggt tcagcgcggc
     6601 gttgatgtcc ggcgccgtgt cgatcagggt gccgtccaga tcgaatagat agccggtgta
     6661 gggcttcaag ctgctccccg cggggtcccg ggttggacgc cctcgagcgc tgcgcgcatg
     6721 gaagcgatgg tggcggcgta gtccgggctg ccgaagatgg ccgagccggc cacgaaggtg
     6781 tctgctcccg cctgggcagc cagcgcgatg ttgtccgcct tgatgccgcc gtctatttcc
     6841 aggcggatgg cacgatcgct gccgtcgatc agcctacggg ccgcggcgag cttgtccagg
     6901 ctggaagcga tgaacgcctg cccgccgaac cccgggttca cggacatgac gagcaccagg
     6961 tcgaccagat cgaggacctc ctcgagaacg cccagcggcg tggccgggtt gaagacgagg
     7021 ccggccctgg cccccccgtc tcgaatcagc gacagcgtac ggtgcagatg ctcggtggct
     7081 tccggatgca cgctgatgat gtcggccccg gcctcgatga agtcgccgac aagccggtcc
     7141 acgggtttca ccatcaggtg cacgtccatg gtgcagtcga tgccggcctt gcgcagcgat
     7201 tgcagcacca gggggccgat ggtgaggttc ggcacgtagt ggttgtccat gacgtcgaag
     7261 tgaatgatgt cggcgccggc gccgagcacg gcttcgatct ccgcgccgag ccgcgagaag
     7321 tcggcggaaa gaatggaggg tgcgatccag tagctcatgg gggcgcattt taccccggtt
     7381 ctgatttgag atgctcgttg gccgcgcgca gctggtcgtg gctgccgatg tcgatccacg
     7441 ttccggtcca gatctcggcc gagacccgcc cgccggccac ggcgtcgaag agaatctgct
     7501 gcagcgagaa aggctcgatg atgcggccgt cgaagagctc gggcctcaag acggcgatgc
     7561 cgcagtagac gtaggactcg ccgcgcccgg tgaccctgcc cgcctcccaa tcgaaatcgc
     7621 cgtgggtacg atgggccggt cggggcgtga gcaccagatg caggtcggcc cagtcgggca
     7681 gggcggtggc gagcgtctgg aaggggaagt cggtgaggat gtcgccgttg agaatcagga
     7741 agggcgcatc gcccagcagg ggcagcgcct tgacgatgcc gccgccggtg tccagcagct
     7801 cgtcctcgcg gctgtagtcg atggccaccc cgtagtcttt cccggatccg cagaacgatg
     7861 cgatctgctc acccagatgg tgcaggttga tcaccacccg ggtgattccg gcggcggcga
     7921 gcgctcgcag ctgccgcgcg atcagcggct cgccggccac cgtcagcatg ggtttcggca
     7981 cggcgtccgt gagcggcttg agccgctcgc cccggcccgc ggcgaggatc atcgctctca
     8041 tcggcgcgcc tgcaggcggc gttcggcggc cttctgccag gcggccagat ggggaccgag
     8101 gggcgtgagc gtgtcgtgcc gggccgacag cgcagcgagg tggtcgagca cgggcgggat
     8161 gtgtcgaaga tgcgacgtct tgccgtccct gaggtcgaga cgggcgaaga tccccaccgc
     8221 tttcagctgc cgctgggcgg cggtgaggtc caggtcgcga gcgaactcct cggggtccag
     8281 cgcgtggggg ctccgttcca gatagtaatc ccgccaccgc gcgatctcgt cctccggaaa
     8341 acggtagtag cagtcccgca gcagcgaggc caggtcgtag ctcacgggcc cgatcagagc
     8401 gtcctggaag tcgacgacgc ccacgctgcc atcggctgcc aggagcaggt tgcggctgtg
     8461 gaagtcccgg tggacgcaga cctgcagctg ctgctcgacg cacgccacga gcctctccgg
     8521 aatgccggcc aggtcctcgg caggaaacgg ctcgccgagc cagcgctcca cgaaccactc
     8581 ccggaagatg ccgagctcgt cccggaaccg ccgagccgtg tagagcggca cggcggggtc
     8641 gtcaacggtc tgaaggcgga tgagcgtgtc gatgccggcg gccagggccc gatcgcgctc
     8701 gccgctctgg taggcgtctg cgaagtgccg ttcgcctaag tcggtcatca gcaggaaacc
     8761 gtccgtgaga tcggcggcga gaatgcgcgg aacccccacg ccatgggcgg cgaagacctt
     8821 ggccagggtg acgaactgct ggttgttctc gcgctcggga ggcgattcca tggccaccag
     8881 gctcagcgcc tccccgccgc gatgccggga gcggatccga tagaagcagc ggtagctcgc
     8941 ctcgaccagg agcgcctggc tggtaaacgt cgggtcaagg tccaggcgag ccagctcacc
     9001 ggcgagccac tgcgcgagtt cggttggtat gctgcgggat tccacccggg caattgtagc
     9061 gcggccgccg ttattcccgg ggaggtaaat cttttatcat ccgcggccat gacgagccgc
     9121 ccttcctcac gacgccgtgc cggatgctgc ctgctcctgg ccggactgct caccggggcg
     9181 gcggctgcag aggacgccgc accggtgacg ggggtggcgg aacggtactg gacgccccgc
     9241 gacgagctgc cggagcgcgt cgcggcccga ttgccttctt actgcgacgg cgcatatctg
     9301 cagccggcct ttccctatcc c
//
LOCUS       DBNHQR010000214         4184 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_971646, whole genome shotgun sequence.
ACCESSION   DBNHQR010000214 DBNHQR010000000
VERSION     DBNHQR010000214.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4184)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_971646"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="AC16VR_04925"
     CDS             <1..228
                     /locus_tag="AC16VR_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010531.1"
                     /translation="LATDGGTLTGTLKGPQGEIELKDGTADGDSLAWKADITTPMAMT
                     LEFSATVNGDEMTGNVKLGAFGDATFTGTRA"
     gene            257..1843
                     /locus_tag="AC16VR_04930"
     CDS             257..1843
                     /locus_tag="AC16VR_04930"
                     /inference="COORDINATES: protein motif:HMM:NF013225.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR6010532.1"
                     /translation="MALRPPQPDADHMSWEEEIRELRKREQLAYQMGGAEKVARQHEF
                     NKLTIRERIAAISDSGSFQEIGTLAGVGQYDDDGNLIGFTPSNFVFGAAEIDGRPVIL
                     SGDDFTVRGGSADASIIGKRLQAEGLATELRLPHVRLVDGMGGGGSVKTIEMAGRTYI
                     PEVRGWETVVQHLAVAPSVSLALGSVAGIGAARVATSHYSVIVKNSAQMMIAGPALVD
                     WASLGDVSKEELGASRIHTRNGAIDDEASSEEEAFDMARRFLSYLPTSTDELPPVTDA
                     TDDPERTDEALLEIVPKDPRKVYKMHRIIDSVVDEGSFFEIGRRWGRSIITGLARLNG
                     MPVALFAENPMVYGGAWTADASRKLVRFIDLASTFHLPLVHLEDCPGFLIGKQSEESG
                     TIRFGCQALAALGQAPVPFCCVVVRKAFGVAGASNHKPGSHCVRMAWPSGDWGSLPIE
                     GGIEVAYKADLAQADDYDAHLSRIKERLNRLRSPFRSAEHFEIEQIIDPRETRPALCR
                     WMKLARQALRTDPPTFWFRP"
     gene            1860..2240
                     /locus_tag="AC16VR_04935"
     CDS             1860..2240
                     /locus_tag="AC16VR_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010533.1"
                     /translation="MGYPATKLTVILIVSAIAAPAFANPVQCTQGSLVRSVEVVYSGP
                     DQTLPCEVIYDKTQEGAGIHSLWRASNEAGYCEAQAAAFADKLGAMGWDCGEPAGSGI
                     DVDSGDAPSAADVPAEIPIAPDPA"
     gene            complement(2245..3285)
                     /locus_tag="AC16VR_04940"
     CDS             complement(2245..3285)
                     /locus_tag="AC16VR_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010534.1"
                     /translation="MSGKISRIARMAVSWAIAAPAIAAPGSLFDEAATLDLELEAPLG
                     ALMGDRYPDSEYRPAQLRYVGKDGSRVALPVEVRTRGKTRRDEEVCKMPPLRVRFDQA
                     AADGTPFAGQPALKLVTYCRDSDSFDQLVLLEYLAYRTYNLLTPQSHRVRLARISYLE
                     RGDRRTTRYGIFLEDWRAVAARNDLTADPVDGGVNVDKLNHDAANRVAVFAYLIGNED
                     YSLLWPEPDENCCHNIKPLLDRDGRVVPLPYDFDYSGLVNAPYAVAKNPRRKVRQRAY
                     RGLCDTQEGLEAALELTRARREDIYAVFRAQEGLSPAKLKSSMGYLDRFYDLIDQPAQ
                     VEKRLRRRCRKS"
     gene            complement(3269..4111)
                     /locus_tag="AC16VR_04945"
     CDS             complement(3269..4111)
                     /locus_tag="AC16VR_04945"
                     /inference="COORDINATES: protein motif:HMM:NF020694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6010535.1"
                     /translation="MNLGVLHPGEMGVSVGAVLTAAGHRVYWLPESRSRATRERAEGA
                     GFTGCASLRELADRVAGIVSVCPPAAAEEVASQVRSAGFTGVYVDANAVSPATARRMA
                     RTMGPTYVDGGIVGPPAVRPGLARLYLSGAAAEEVAGWFHGGLLEARVLAGEAGAASA
                     LKMCYAAYTKGSSALVLAIRALAEAEGVDEPLLAEWDISQPGLADRSQRAAAGTAAKA
                     WRFVGEMEEIAATFRGVGLPGEFHEAAAVVYRRLAPLKGNAAAELADVVRRLIENPAD
                     VGED"
BASE COUNT          723 a         1424 c         1380 g          657 t
ORIGIN      
        1 ctggccaccg acggcggcac cctgacgggc acgctgaaag gaccccaggg cgagatcgag
       61 ctcaaggacg gcacggcaga cggcgactcg ctggcctgga aggctgacat cacgacgccc
      121 atggcgatga cgctggagtt cagcgcgacc gtaaacggtg acgagatgac cggaaacgtc
      181 aagctcggcg ctttcggcga cgccacgttt accggaaccc gcgcctgacg gatccgagcc
      241 cgagcgaacc ggctgcgtgg cgcttcgccc accgcagccg gacgccgacc acatgagctg
      301 ggaggaggaa attcgcgagc tgcgcaagcg cgagcagctc gcctatcaga tgggcggcgc
      361 cgagaaggtg gcgcgccagc acgaattcaa caagctgacg atccgcgagc ggatcgccgc
      421 catcagcgac tccggctctt tccaggagat cggcaccctg gccggcgtcg ggcagtacga
      481 cgacgacggc aatctcatcg ggttcacccc ctccaatttc gtattcggcg ccgcggagat
      541 cgacggccgg ccggtgattc tctcaggcga cgacttcacg gtgcgcggcg gctccgcgga
      601 cgcgtcgatc atcggcaaac ggctccaggc agagggcctg gccaccgagc tccggcttcc
      661 ccacgtgcgt ctggtggacg gcatgggcgg cggcggatcc gtgaagacca tcgagatggc
      721 cggccgaacc tacatacccg aggtgcgcgg ctgggagacc gtcgttcagc acctggcggt
      781 tgccccatcg gtctctctgg cgctgggctc cgtggccggt atcggcgctg cccgggtggc
      841 gaccagccac tactccgtca tcgtcaagaa cagcgcccag atgatgatcg cgggacccgc
      901 actggtggat tgggcaagcc tcggcgatgt cagcaaggaa gagctcggcg cctctcgcat
      961 ccatacccga aacggggcca tcgacgacga ggcgtcttcc gaggaagaag cgttcgacat
     1021 ggcacggcga tttctctcct acctgcccac cagcacggac gagctgccgc ccgtcaccga
     1081 cgccacggac gatcccgagc ggacggacga ggcgctgcta gagatcgttc cgaaggatcc
     1141 gcgcaaggtc tacaagatgc accggatcat cgactcggtg gtcgacgagg ggtcgttctt
     1201 cgagatcggc aggcgttggg gccgatccat catcaccggt ctggcccggc tgaacggcat
     1261 gccggtggcc ctttttgcag agaacccgat ggtgtacggg ggcgcgtgga cggcggatgc
     1321 gagccgcaag ctggtgcgtt tcatcgatct ggccagcacg ttccacctgc cgttggtgca
     1381 tctggaggat tgccccggat ttctgatcgg caagcagtcc gaggagagcg gaaccatccg
     1441 cttcggttgc caggcgctgg ccgctctggg ccaggcaccc gtccccttct gctgcgtggt
     1501 ggtgcgcaag gcgttcgggg tcgctggcgc ttccaaccac aaacccggca gccattgcgt
     1561 acgcatggcg tggccgtcgg gagactgggg ctcgcttccc atcgagggcg gcatcgaggt
     1621 ggcctacaag gccgacctcg cccaggccga cgattacgat gctcacctca gccggatcaa
     1681 agagcgcctc aaccggctgc gctcgccgtt ccgttctgcg gagcacttcg agatcgagca
     1741 gatcatagac ccccgcgaaa cccgccccgc gctgtgccga tggatgaagc tggcgcggca
     1801 ggcactgaga accgatccgc cgaccttctg gttccggccg tgatctcaag gagcaactca
     1861 tgggataccc agcaacgaaa ctgaccgtca tcctcatcgt ctcggccatc gccgctccgg
     1921 cgttcgccaa tccggtgcaa tgcactcagg ggagcctggt tcgcagcgtc gaggtcgtct
     1981 acagcggtcc cgaccagacc cttccctgcg aagtgatcta cgacaagact caggaaggcg
     2041 cgggaatcca ctcgctgtgg cgggcatcca acgaagccgg ctactgcgag gcccaggcgg
     2101 ccgctttcgc agacaagctc ggcgccatgg gctgggactg cggcgagccg gcgggatccg
     2161 gaatcgacgt cgactccggt gacgccccgt ccgccgcgga tgtccctgca gagataccca
     2221 tcgcgccgga ccccgcctga tcgttcagct ttttctgcac cgacggcgca ggcgtttttc
     2281 gacctgcgca ggctggtcga tcaagtcgta gaaccggtcg agatagccca ttgacgactt
     2341 cagctttgcc ggcgacaacc cttcctgggc gcggaagacc gcgtagatgt cctcgcgccg
     2401 cgcacgcgtg agctccagcg ctgcctcgag accttcctgg gtgtcgcaaa gaccccggta
     2461 ggctcgctgg cgaacttttc tgcgcggatt cttggccacg gcataaggcg cgttgacgag
     2521 cccggaatag tcgaaatcgt agggcagcgg caccacccgg ccatcccggt cgagcagcgg
     2581 cttgatgttg tggcagcagt tctcgtcggg ttccggccag agcagtgagt agtcctcgtt
     2641 gccgatcagg tacgcaaaga ccgccacgcg gttggcggcg tcgtggttga gcttgtcgac
     2701 gttcacaccg ccgtcgaccg ggtcggcggt gaggtcgttg cgggctgcca ctgcccgcca
     2761 gtcctctaag aagatgccgt agcgggtcgt tcgccggtcc ccacgctcga ggtagctgat
     2821 gcgcgcaagc cggacccgat gcgactgcgg ggtcagcaag ttgtaggttc tgtaggcaag
     2881 gtactcgagc agcacgagct gatcgaagga gtcgctgtcg cgacagtaag tgaccagctt
     2941 tagtgcgggc tgaccggcga acggcgtacc gtccgcggcg gcttgatcga agcgcacacg
     3001 cagcggcggc atcttgcaca cttcctcgtc ccggcgggtt ttcccacgtg tccggacctc
     3061 aacgggcagc gcgacgcggc taccgtcctt gcccacgtag cggagctgcg caggccggta
     3121 ctcgctgtct gggtagcggt cacccatgag cgctccgagg ggggcctcca gctcgaggtc
     3181 gagcgtggcc gcctcgtcga acagagagcc cggggccgcg atggccgggg ccgcgatggc
     3241 ccacgacacg gccatccggg cgatgcggct aatcttcccc gacatccgcg ggattctcga
     3301 tgagccgtcg aaccacgtcg gcgagttctg ccgccgcgtt ccctttcaac ggtgccagac
     3361 gccggtagac aacggccgcc gcctcgtgaa actcgcccgg caatcccacc ccgcgaaagg
     3421 tggccgcgat ttcctccatc tcgccgacga accgccaggc tttggccgcc gtgcccgcgg
     3481 ccgcacgctg gctccgatcg gcaagaccgg gctgagagat gtcccactcg gccagcagcg
     3541 gctcgtccac cccctcggcc tcggccagcg cccggatggc cagcacaaga gcgctgctgc
     3601 ccttggtata tgccgcgtaa cacatcttca gcgcggacgc cgcgcccgcc tcgcccgcca
     3661 ggacccgggc ctcgagaagg ccgccatgaa accatcccgc cacttcctcg gccgcagctc
     3721 cagacaggta cagccgcgca aggccgggac ggaccgcggg agggccgacg atcccgccgt
     3781 cgacgtacgt gggacccatc gttcgagcca tccgcctcgc cgtggcggga gacaccgcat
     3841 tggcatcgac gtagacgccg gtgaagcccg ccgagcggac ctggctcgcc acctcttcgg
     3901 ctgccgccgg aggacagacg ctgacgatgc cggcaacgcg gtccgcaagt tcccggagcg
     3961 aggcgcagcc ggtgaagccg gcgccttcgg cgcgctcgcg ggttgcgcgg ctgcgagact
     4021 cgggaagcca gtacacccgg tgccctgcgg ccgtcagaac ggcgcccacg ctgacgccca
     4081 tctccccggg gtgcaggacc ccgaggttca ccgccggtaa tccaaaggcg gatcgcgctc
     4141 gccgagcagg gcaacgtagc ggaagtcctg gccgcgacgc tctt
//
LOCUS       DBNHQR010000215         5806 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_401185, whole genome shotgun sequence.
ACCESSION   DBNHQR010000215 DBNHQR010000000
VERSION     DBNHQR010000215.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5806)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5806)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5806
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_401185"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..534
                     /gene="mlaD"
                     /locus_tag="AC16VR_04950"
     CDS             76..534
                     /gene="mlaD"
                     /locus_tag="AC16VR_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HIR6010536.1"
                     /translation="MHIRTLEISVGAFMLAGILALVFLAVKVSGISLDADRGSYQLVA
                     RFDDVAGLRERAKVSIAGVQIGRVALIEVDTIYGQAVVTLEIDGSPGGLSADTGAQIL
                     TEGILGARYVNLLPGADDETLADGDLIYDTQGALVLENLVGDLITRLGAG"
     gene            534..1298
                     /locus_tag="AC16VR_04955"
     CDS             534..1298
                     /locus_tag="AC16VR_04955"
                     /inference="COORDINATES: protein motif:HMM:NF017319.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaC/ttg2D family ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR6010537.1"
                     /translation="MTMRLMNHSGLESGFVLAIAALLLAGLAPARAMEPSDVATRTAT
                     EVVERTSDEMLALIDASRPYVEDDPERFYGEVESLLAPVVDFKRFARSVMAVHYKSAT
                     PEQRDRFADTFKWGLVRTYALALTEFRDGEVVIVPSERPPQSPRRESVKMEIRMNTGD
                     VYPVVYSMAQGKDGAWRMGNIIVNGVNIGLTYRSQFASAVQDQRYGGDLDKVIDAWTL
                     LLANETPGAASADSGGSDEADEAPSSDGAGGAGDDR"
     gene            1295..1585
                     /locus_tag="AC16VR_04960"
     CDS             1295..1585
                     /locus_tag="AC16VR_04960"
                     /inference="COORDINATES: protein motif:HMM:NF024857.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR6010538.1"
                     /translation="MSAFSLPPRVGVDNFAAVRAAGEQFLAERRDEKAVVDLSGLESC
                     NSIAVALLMTWFRFAHARGQEVVYAGVPQDLLSIIEISGLMEVLPLSRDAAG"
     gene            1615..1866
                     /locus_tag="AC16VR_04965"
     CDS             1615..1866
                     /locus_tag="AC16VR_04965"
                     /inference="COORDINATES: protein motif:HMM:NF013851.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HIR6010539.1"
                     /translation="MQEEIAASITERLSGEDDALRVDVVVEGNRAMITVVSRMFEGMT
                     RVRKQQTVYGCIDAFIQDGRLHAVSIRSLTPEEAAGSSG"
     gene            1859..3124
                     /gene="murA"
                     /locus_tag="AC16VR_04970"
     CDS             1859..3124
                     /gene="murA"
                     /locus_tag="AC16VR_04970"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009398241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIR6010540.1"
                     /translation="MDKLRITGGRTLSGSLRVSGAKNAALPILAGTLLADEPVTLSNV
                     PHLQDVTTMIELLGCMGARVLVDEKMRVEVSADSLAQLRAPYELVKTMRASFVVLGPL
                     LARFGEAEVSLPGGCAIGARPVDQHVKGLEALGAEISVNEGYVRGKVGGRLRGADFAF
                     DMVTVGATENLMMAAALAEGVTRLRNAACEPEVADLGDFLNAMGAKVSGHGSPEIRIE
                     GVPRLHGCEYRVMPDRVEAGTYLIAVAATGGRIRLTDVRPDTLTAVLDKLVESGAEIE
                     VGDDWIELDMHGRRPRAVDITTAPYPGFPTDMQAQYLAMNAIAEGTSEVSETIFENRF
                     MHVQELSRLGADIELHGPSSVSVRGVPSLKAAPVMATDLRASFSLVVAALVAEGTTLI
                     DRIYHIDRGYETIEEKLKLLGAEVQRVSG"
     gene            3140..3766
                     /gene="hisG"
                     /locus_tag="AC16VR_04975"
     CDS             3140..3766
                     /gene="hisG"
                     /locus_tag="AC16VR_04975"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009617828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HIR6010541.1"
                     /translation="MGLTIALNRGRILEESLPMLAAAGIVPEEDPAASRKLIFATRQG
                     GHRLVVMRGADVPTYVEYGAADVGIVGKDTLLEHGGSADYYERLDLGIAKCRIMVAAP
                     AGADLGSGKLRVATKFPRVARRYFEGQGRQVSIIKLNGAMEIAPLMNLADCIVDIVDT
                     GNTLRANGLEALETIAHVSSRMIVNRAAMKTRFDAVQSLTCSLRDVSG"
     gene            3763..5091
                     /gene="hisD"
                     /locus_tag="AC16VR_04980"
     CDS             3763..5091
                     /gene="hisD"
                     /locus_tag="AC16VR_04980"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HIR6010542.1"
                     /translation="MTEPALEIARLATGDADFPDRLARLLYWDTGLEDEVTRAAAEVI
                     EAVRAGGDDALLALTARYDRYECPSAAALEIAASELEAAYDALGSADREALNLAAQRI
                     REYHNHQLAGDWEYTDSDGNLLGQRVMALERVGLYVPGGQAAYPSTVLMTAVPARVAG
                     VDEIIMTVPTPDGVRSPLVLAAAHVAGVDRVFTVGGAQAIAALAYGTPTIPKVDKIVG
                     PGGAYVAAAKRLVFGTVGIDVIAGPSEVLVIADDTAPPEWLALDLFSQAEHDASAQAI
                     LVSPDPDVLDAVHDAMASLIGERSRATTIRASLAARGALIRAADLADAVALANRIAPE
                     HLELAVADPDALLPDVRNAGAIFIGAHSPEVMGDYVAGPSHVLPTFGTARFSSPLGVY
                     DFVKRSSVIRLSPGGSEKLARASATLARGEGLEAHARAAELRITGLDHNR"
     gene            complement(5109..>5806)
                     /locus_tag="AC16VR_04985"
     CDS             complement(5109..>5806)
                     /locus_tag="AC16VR_04985"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR6010543.1"
                     /translation="ATTATLVASDPETDLAVIRVQASGLTPVPRDPSSVVEVGDVALA
                     IGNPFGIGQTVSSGIISAKGRAGISRSPYEDFIQTDAAINPGNSGGALIDANGNLIGI
                     NTLIFSRSGGSEGIGFAIPAQLAMAVLEEIIETGRVVRGYLGVELSSTPPAGAGVGLE
                     VTRLMSPSPAASAGLRPGDLILSINEQPAVSAAGVTRQIAHVEPGSELEIEVLRGNRQ
                     ISLTARAGERPQP"
BASE COUNT          965 a         1847 c         2007 g          987 t
ORIGIN      
        1 ggtcgtggcc tcctcggtga tggtgctcgc aatcgacttc ttgctcacgc tgagcatgta
       61 cggagagttc aactgatgca catacgaacc ctagagatca gcgttggcgc gttcatgctt
      121 gctggaatcc ttgcgctcgt ctttctcgcc gtgaaggtca gcggcatcag tctggatgca
      181 gatcgtggca gctatcagtt ggtggcgcgg ttcgacgacg tggccggcct gcgcgagcgt
      241 gccaaggtga gcattgccgg tgtccagatc gggcgcgtgg cgctcatcga ggtcgacacc
      301 atctacggcc aggccgtggt gaccctcgaa atcgacggca gtccgggcgg cctttccgcc
      361 gacacggggg cgcagatact caccgaagga attctcggtg cgcgctacgt gaatctgctg
      421 ccgggggcag atgacgagac gctggcggac ggcgatctga tttacgatac ccagggcgcc
      481 ctcgtgctgg agaacctggt cggcgacctc ataacgcggc ttggagcagg ctgatgacga
      541 tgcgtttgat gaatcactcg ggtcttgaat ccggcttcgt gctcgcgatc gccgccctgc
      601 tgctcgcagg cctcgcgccc gctcgcgcca tggagccctc ggacgtcgcg acccgtaccg
      661 ccaccgaggt ggtggagcgc acgtcggacg agatgctcgc gctcatcgat gcatccagac
      721 cgtacgtcga ggatgacccg gaacggttct acggcgaggt ggagtcgctg ctcgcgccgg
      781 tggtcgactt caagcggttt gcccgcagcg tcatggccgt gcactacaaa tccgccactc
      841 ccgagcagcg cgatcgcttc gccgatacct tcaaatgggg cctggttcga acctatgcgc
      901 tggcgctgac ggagttcagg gacggcgagg tggtcatcgt gccctccgag cggccgcccc
      961 aaagcccccg ccgcgagagc gtgaagatgg aaatccgcat gaacaccgga gatgtatacc
     1021 cggtggttta ctcaatggcg cagggcaagg acggcgcctg gcgcatgggg aacatcatcg
     1081 tcaacggcgt gaacatcggt ctcacctacc gcagccagtt tgccagcgcc gtgcaggatc
     1141 agagatacgg gggggacctg gacaaggtca tcgacgcctg gactttgctc ctggccaacg
     1201 agacaccggg tgccgcgtcg gctgactcgg gcgggtcaga cgaggcggac gaggcgccgt
     1261 cgagcgacgg ggccggcgga gctggtgacg atcgttgagc gcgttttccc tgccgcctcg
     1321 cgtcggcgtc gacaacttcg ctgcggtcag ggcggctggc gagcagtttc tcgccgaacg
     1381 gcgcgacgag aaagccgtcg tcgatctttc cggcctggag agctgtaaca gcatagccgt
     1441 cgcccttctg atgacctggt ttcgctttgc ccatgcgcgg gggcaggagg tggtttatgc
     1501 tggcgtcccc caggatttgc tcagcatcat tgaaatttcc gggctgatgg aagttctgcc
     1561 gctttcccgg gacgcggcag gctgaacctt taaccgactg cggcagggcg ggacatgcag
     1621 gaagaaatcg cggcgagcat cacagaaagg ctgtccgggg aggacgacgc cttgcgcgtg
     1681 gacgtcgtcg tcgaggggaa ccgcgcgatg atcaccgtgg tcagccggat gttcgagggc
     1741 atgacgcgcg ttcgcaagca acagaccgtt tacggatgca tcgatgcttt catccaggac
     1801 ggtcgcctgc acgccgtgag cattcgctcc ctcacgcccg aggaggcggc gggaagcagt
     1861 ggataagctc cgcataacgg gcgggcgcac gctgtccggc tcgctgcggg tctctggcgc
     1921 aaaaaacgcg gcgctgccga tcctggcggg cacgcttctg gccgacgagc cggtgacgtt
     1981 gagcaacgtc cctcatctcc aggacgtcac caccatgatc gagctgctcg gctgcatggg
     2041 cgcccgcgtg ctcgtcgacg agaagatgcg cgtcgaggtt tcggccgaca gcctggcgca
     2101 actgcgggcg ccctacgagc tggtgaaaac catgcgcgcc tcgttcgtcg tgctcgggcc
     2161 gctgttggcg cgcttcggcg aagcggaggt ctcccttccg ggcggttgcg ccatcggggc
     2221 ccgccccgtg gaccagcacg tcaaaggcct ggaagccctc ggtgcggaga tctccgtgaa
     2281 cgagggttac gttcggggca aggtcggcgg ccggctccgc ggggccgact tcgcgttcga
     2341 catggtgacg gtgggcgcaa ccgagaacct catgatggcg gccgcgctgg ccgagggggt
     2401 cacgcggctg cgcaacgccg cctgcgaacc ggaagtggcc gacctcggag attttctcaa
     2461 cgccatgggc gcgaaggttt cgggtcacgg ctccccggaa ataagaatcg aaggcgtgcc
     2521 gcggttgcac ggatgcgagt accgggttat gcccgatcgc gtcgaagccg ggacttacct
     2581 gattgccgtt gcggccaccg ggggcaggat aaggctcacc gacgttcgac ccgacaccct
     2641 tacggccgtg ctcgacaagc ttgtggaaag cggcgccgag atcgaagttg gcgacgactg
     2701 gatcgagctc gacatgcacg gccggcgacc gcgcgcggtg gatatcacca cggcgccata
     2761 tcccggcttt ccgacggaca tgcaggccca gtacctggcg atgaacgcca tcgccgaggg
     2821 aaccagcgag gtctcggaaa ccatcttcga gaaccgcttc atgcacgttc aggagctctc
     2881 gcgactcggc gcggacatcg agctgcacgg cccgtccagc gtctcggtgc gcggcgtgcc
     2941 gtcgctgaaa gccgctcccg tcatggccac ggacttaaga gcgtcgttca gcctggtggt
     3001 cgccgcgctc gttgcggaag gcaccacgct catcgatagg atctatcaca tcgaccgcgg
     3061 ctacgagacc atcgaggaga agctcaaact gctcggcgct gaggtccagc gggtttcggg
     3121 ctgatcgaga ggaattgaaa tgggtctgac catagcgctc aaccggggca ggatcctcga
     3181 ggagtccctg ccaatgctgg ctgcggcggg catcgtcccg gaggaggacc ctgccgccag
     3241 ccgcaagctc atttttgcca cccggcaggg cgggcatcgc ctggtggtga tgcgtggtgc
     3301 cgatgtcccc acctacgtgg aatacggggc ggcggacgtg ggcatcgtgg gcaaggacac
     3361 cctgctggag cacggcggca gcgcggatta ctacgagcgc ctggacctgg gcatcgcgaa
     3421 gtgccgcatc atggtggctg ctcccgcagg cgcggatctg ggatccggca agctgcgggt
     3481 ggcgacgaag tttccgcggg tggcccgtcg ctacttcgaa ggtcaggggc gtcaggtatc
     3541 catcatcaag ctaaacggcg ccatggagat cgccccgctc atgaacctgg cggattgcat
     3601 cgtggacatc gtggacaccg gaaacacctt gcgcgcgaac ggcctggaag ctcttgagac
     3661 cattgcgcac gtctcctcgc gaatgatcgt aaaccgggcg gccatgaaga cccgcttcga
     3721 cgccgtgcag agcctcacct gcagcctgcg ggacgtgtcg ggatgacgga accggcgctc
     3781 gagattgccc ggctcgcgac gggcgatgcg gacttcccgg atcgtctcgc caggctcctt
     3841 tactgggaca cgggcctgga agacgaggtg acgcgagcgg cggccgaggt catagaagcg
     3901 gtgcgggccg gtggtgacga cgccctgctg gcgctcaccg ccaggtacga tcgttacgag
     3961 tgcccgagcg ccgcggcgct ggagatcgcg gcttccgagc tcgaggcagc ctacgacgcc
     4021 ctgggctcag cagaccgcga ggcgttgaac ctggccgccc agcggattcg cgaatatcac
     4081 aaccaccagc tggccggaga ctgggaatat accgacagtg acggcaacct cctcggccag
     4141 cgggtgatgg cgctggagcg ggtgggcctc tacgtgcctg gcggccaggc ggcgtacccg
     4201 tcaacggtgc tgatgacggc ggtgcccgcc cgggtcgcgg gtgtggacga gatcatcatg
     4261 accgtcccca cgccggatgg cgtgcgttcg ccgctggtgc tcgccgcagc ccatgtagcg
     4321 ggcgtcgacc gggtatttac cgttggcggc gcccaggcca tcgccgcgct ggcctacggc
     4381 acgccgacga ttcccaaggt ggacaagatc gtcggtcccg gcggggcata cgtcgctgcc
     4441 gccaagcgcc tggttttcgg caccgtcggt atcgacgtca ttgccggccc cagtgaggtg
     4501 cttgtgattg cggacgacac ggcgccgccc gagtggctcg ccctggacct gttttcccag
     4561 gcggagcacg atgcttccgc ccaggccatt ctggtcagcc cggacccgga cgtgctcgat
     4621 gccgtccacg acgccatggc ctcgctcatc ggcgagcggt ccagagcaac gaccatccga
     4681 gcctcgctcg ccgcgcgagg tgccttgatc cgggcggcgg acctcgccga cgccgtggcg
     4741 ctggccaacc gaatcgcgcc cgagcatctc gagctcgccg tggcggatcc ggacgcgctc
     4801 ctgcccgacg ttcgcaacgc cggcgcgatc ttcatcggcg cgcactctcc ggaggtgatg
     4861 ggcgattacg tggcgggtcc gagccacgtg ctgccgacat tcggtacggc caggttctcg
     4921 tcaccgctgg gggtctacga tttcgtcaaa cgcagctccg tgatccggct gagtccaggc
     4981 gggtccgaaa agcttgcccg cgcctcggcg accctcgctc ggggcgaggg gctcgaagcc
     5041 cacgccaggg ccgctgaatt gaggatcacc gggctggacc acaaccgtta gccggacccg
     5101 tgaggctcct agggctgagg gcgctcgccg gcgcgcgccg tgagggagat ctggcgattc
     5161 ccacgcagaa cctctatctc cagttcgctg cccggctcga cgtgagcgat ctgccgcgtg
     5221 acgcctgccg cgctcacggc cggttgctcg ttgatggaaa ggatcagatc gcccgggcgc
     5281 agaccggccg aagcggcggg gctgggtgac atcagtctgg tgacttccag gccgacgcct
     5341 gccccggcag gcggcgtgct ggacagctcg acgccgagat agccgcggac cacgcggccc
     5401 gtctcgatga tctcctccag caccgccatg gcaagctgtg cggggatggc gaacccgatg
     5461 ccctcggagc cgccggagcg ggaaaagatc agcgtgttta tgccgatcag gttgccgttg
     5521 gcatcgatga gcgcgccccc ggagtttccg gggttgatgg ctgcgtcggt ctggatgaag
     5581 tcctcgtagg ggctgcggct gatgcccgcc cggccctttg cgctgatgat gccgctcgag
     5641 accgtctggc caatgccgaa cgggttgccg atggccagcg cgacgtcgcc cacctcgacg
     5701 acgctgctcg gatcacgggg caccggcgtc aggcccgagg cctgcacgcg gatgacggcg
     5761 agatcggtct cggggtcgct ggcgaccagg gtcgccgtgg tggcct
//
LOCUS       DBNHQR010000216         7170 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_845239, whole genome shotgun sequence.
ACCESSION   DBNHQR010000216 DBNHQR010000000
VERSION     DBNHQR010000216.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7170)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7170)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7170
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_845239"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="AC16VR_04990"
     CDS             complement(<1..555)
                     /locus_tag="AC16VR_04990"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6010544.1"
                     /translation="MAYNTILYDVEDGVLTLTLNRPERLNAFNHEMLSEMIDAFDRAD
                     ADDEVSSIIVTGAGRAFCAGADLGAGGDTFNADGREDRASGLHPDGGGRLTLRIYELK
                     KPIIAAVNGPAVGVGVTMTLAMDIRLAADVAKFGFVFARRGIVPEACSSYFLPRIVGI
                     SQALEWCYSGRVFPAEEALAGGLVR"
     gene            680..1396
                     /locus_tag="AC16VR_04995"
     CDS             680..1396
                     /locus_tag="AC16VR_04995"
                     /inference="COORDINATES: protein motif:HMM:NF013488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR6010545.1"
                     /translation="MPSTSSFDPLGSSSGLIVYVDFKSPYAYLAKDPTFQLADDLGID
                     LDWRPLTLDINSYLGSARLNASGAVAESRRTPEQWHRVRYAYRDARRYANLVGLTVRG
                     TTKIWDSSLASIGLQWAKGQGAGPLRAYIDLTYERFWKRELDIEDASVVAGVLAEAGA
                     DPDGFVDFARGEGRAEHDVMQQRIFAAGIFGVPSYVLDGELYFGREHLPRIRWLLTGR
                     PGPCPDVAYRHFDAQETIRG"
     gene            1389..2051
                     /locus_tag="AC16VR_05000"
     CDS             1389..2051
                     /locus_tag="AC16VR_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010546.1"
                     /translation="MAETEAYTFTAALTVCLDITQPESYLAVPGVRELAADLGMDVRW
                     LPYSVPPWPARSEPGPEADRGTWHRWHRARYRERDLRRYARLLSIDLGDLHRQIKGEL
                     AGLALLWVQDQPAAVGGALLDGLLEGHWSGALNIEEPASVTAMLVQAQARVAGWDEYR
                     SGRGPVELAALRSALSAAGVFDVPSLVVAHAVPEPEVFLGRAHLPMVRWLLTDRQGAP
                     PI"
     gene            complement(2053..2676)
                     /locus_tag="AC16VR_05005"
     CDS             complement(2053..2676)
                     /locus_tag="AC16VR_05005"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6010547.1"
                     /translation="MQDDALPPQLFARADESPDSAFYREPRFVTHIDDATIDCLTAFY
                     RTFIPENAAVLDLMSSWVSHLPPECSYARVAGLGMNEAELAANPRLDDHVVHDLNAAP
                     ELPYPDSAFDRVILAVSIQYLTRPVEVLREALRTLREDGEICIAMSHRLFPTKAILAF
                     HRLGPADRVRLVSTYLELAGFLDVRFVDRSPDGADPLWLVVGRRAGR"
     gene            2874..4067
                     /locus_tag="AC16VR_05010"
     CDS             2874..4067
                     /locus_tag="AC16VR_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6010548.1"
                     /translation="MRFTEDDLAFQAEVRSFIDRHWPADVRLARATVPAHGLDRPAEE
                     QAWFDALVARGWSVPNWPLEHGGTDWTPTQRFIWDRETVRAETPQMSVFGVTMLGPIL
                     CRWGSVDQQEKFLPPIREARVQWCQGYSEPGAGSDLASLKTTARLDGDHYTVDGEKTW
                     TSGAHVADWMFCLARTSSTNKPQEGISFLLIDMRSPGVEVHPIEILGGQHSVNSVRLD
                     GVRVPVAHRVGEENQGWTYAKALLSHERTGVARVATSGVQLNRVREIAEGQEPSTDGL
                     WQDAGFQRRYRELEIELIGLEMLELRILADLEAGQVPGPESSILKIKGTEIGQRINEL
                     MVEALGYYALPYPDELLIDNEGPVGHDHALAALKGWLFSRAWSIYGGSNEVQKNIIAR
                     RALGL"
     gene            4077..5207
                     /locus_tag="AC16VR_05015"
     CDS             4077..5207
                     /locus_tag="AC16VR_05015"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6010549.1"
                     /translation="MDFSFSDEQTLLQDSIEKFIQNDYAFDARQRVVREEQGFSSDNW
                     RTFAELGWLGVPFPEADGGFGGTAIESMIMSEQFGKGLVVEPFLATVVLAGGALKIGG
                     SASQKEKYLAGVMDGSLQGALAYAEPQGRFNLADLTTTASPEGDGYVINGYKAVVMNG
                     PSADFLVVSCRTGGGQRDADGVSLFVLPADTEGVARRDYPTVDAFRASEVTLENVRVG
                     SDALIGEEGKGLAILEQVVDEAILAVGAEAVGCMEVLYKTTVEYCKTREQFGQPIGKF
                     QVLQHRMVDMFMEHEQAKSLMYMAAMRLDEGYGPEAQKAVSAFKVQVGKSGRFVGQNA
                     VQLHGGMGMTEELNVGHYFKRLTMIDTLFGNVDFHLKRFGSL"
     gene            5211..6098
                     /locus_tag="AC16VR_05020"
     CDS             5211..6098
                     /locus_tag="AC16VR_05020"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6010550.1"
                     /translation="MARWDRDSGASIPDWFFEAVETEYATRSVEVDECDVVYQLWGDE
                     DKPGILLIHGMNAHSHWWDFIAPQLLDGYRVAAMNLTGMGDSDYRYEYDGATYAQEIR
                     AVCDHAGFDERAAQVAHSFGGYMAVKAAVLFPDRFGALVLVDSGIRHPEEPVPDRPAM
                     GGRSKVYPDRETALGRFRVQPPQSCDNAYILEYIARHSLMPVDGGWAWKFDEDLPSTL
                     KDVERTPEDYRHLDVPTGLIYGAESELFSARTEDYMRELIAQDFPAVALPEAQHHLFL
                     DQPLAFVAELRKMLATLLA"
     gene            complement(6115..>7170)
                     /locus_tag="AC16VR_05025"
     CDS             complement(6115..>7170)
                     /locus_tag="AC16VR_05025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6010551.1"
                     /translation="AESRLIPPGVAWHRDSATGFDPESNSVTLASGARLSYDYLVVCP
                     GLELNWERIDGLRETLGSNGVCSNYSPDHVEYTWQCLQSLQEGATALFTQAPLPFKCP
                     GAPQKIAYLAADHLQRRGLLGSCHLHFLTHGPALFAVPLFAAELSKVAERWGIDVHFQ
                     TNLIRVDGPARQATFEKVGGEGEGEEVTMTFDMLHATPYQSPPAAVKESPLANEAGWV
                     DVHQHSMQHTTYANVFGLGDACSTPNSKTAAAVRKQAPVVVRNLLHLLDGGSVEEAYD
                     GYGSCPLTTAYGKVLLAEFTYGGKVTPTFPLDPRKERRSMWWVKKTALPIMYWDYMLR
                     GHEAFPGHDTEYEAPPV"
BASE COUNT         1209 a         2256 c         2433 g         1272 t
ORIGIN      
        1 gcgcaccagc ccgccagcca gagcctcctc ggcggggaac acgcggccgc tgtagcacca
       61 ttccagtgcc tggctgatgc ccacgatgcg tggcaggaag tagctcgagc aggcttcggg
      121 gacgatgccc cggcgtgcaa agacgaagcc gaacttggcc acgtctgcgg ccaggcggat
      181 gtccatggcc agcgtcatgg tcacgcccac gccgacggcg ggcccgttca ccgcggcgat
      241 gatcggcttc ttgagctcgt agatccgcag ggtcaggcgg ccgccgccgt ccgggtgaag
      301 gccgctggct cggtcctcgc gaccgtccgc attgaaggtg tcgccgccgg cgccgaggtc
      361 cgcgccggca cagaatgccc gaccggcccc tgtgacgatg atgctgctca cctcgtcgtc
      421 tgcatctgcc cggtcgaagg cgtcgatcat ctcgctgagc atctcgtggt tgaacgcgtt
      481 cagacgttcc ggccgattta gggtcagcgt cagaacgccg tcttcgacgt cgtaaaggat
      541 cgtgttgtag gccatgtgcg ggggcgtccc cagatgagta gacagtggcg catgttgcct
      601 agattcctgc ggattttcca tgcggactgg catgcgacct ggtttggcgc gccgcgcaac
      661 ccctactaca atcgcccgaa tgccgtccac atcctctttc gacccgctcg gcagctcatc
      721 ggggctcatc gtctacgtcg acttcaagag cccttatgcc tacctggcca aggaccccac
      781 attccagctg gccgacgatc tcggcatcga cttggactgg cggccgctca cgctcgacat
      841 caacagctac ttaggctctg cgcggttgaa cgcctcgggt gcggttgccg agagtcgccg
      901 tacgcccgag caatggcacc gggtcaggta cgcctatcgc gatgcccggc gttacgcgaa
      961 cctggtgggc cttaccgtca ggggcaccac gaagatctgg gattcgtccc ttgcatcaat
     1021 tggtctgcag tgggccaaag ggcagggagc cggcccgctg cgggcctata tcgatctgac
     1081 ctacgagcgg ttctggaagc gcgagctcga catcgaggac gcatccgttg ttgccggcgt
     1141 gctcgcagag gcgggagcgg accccgacgg gttcgtcgac ttcgcccgcg gcgaggggcg
     1201 tgccgagcac gatgtcatgc agcagcgtat cttcgccgcc ggcatcttcg gcgtgccgag
     1261 ctatgtgctc gacggcgagc tctacttcgg cagggaacac ctgccgagaa tacgctggtt
     1321 gcttacaggc cgaccgggcc cctgtccgga cgtggcctat cgtcacttcg atgcgcaaga
     1381 aacgatccgt ggctgagacc gaggcttaca cgttcaccgc cgcgctcacc gtctgcctgg
     1441 acatcaccca gccggagtcc tatctggccg ttccgggcgt gcgcgagctc gccgccgatc
     1501 tgggcatgga cgtgagatgg ttgccctatt cggtgccgcc ttggcctgca cggtccgagc
     1561 cgggccccga agcggatcgg ggaacctggc accgctggca tcgtgcgcga tatcgggagc
     1621 gggacctgcg ccgctacgcc cgtcttctca gcatcgatct gggggacctg caccggcaga
     1681 ttaagggcga gcttgccggg ctcgcgctgc tctgggtgca ggatcagccg gccgccgtgg
     1741 gcggcgcttt gctcgacggc ctgctggagg gtcactggag cggggcgctg aacattgagg
     1801 agccggcatc ggtgaccgcg atgctcgtcc aggctcaggc ccgcgttgcc ggctgggacg
     1861 agtatcgcag cgggcgcggc cccgtcgagc tggcagccct gcgctccgcg ctcagcgcag
     1921 cgggggtttt cgatgtccct tcgctcgtcg tggcccacgc ggtgcccgag cccgaagtct
     1981 tccttggtcg cgcgcatctg ccgatggtcc gctggttgct cacggatcgg cagggcgcgc
     2041 cgcccatctg attcagcgcc cggcgcgacg tccgacgacc agccacaggg gatctgcgcc
     2101 gtcgggggat cggtcgacga agcgcacgtc cagaaaaccg gcgagctcca gataggtgga
     2161 taccagccgc acgcggtcgg cggggccgag cctgtggaag gcgaggatcg ccttggttgg
     2221 aaacaggcgg tgggacatgg cgatgcagat ctcgccatcc tctctgaggg tgcgcagggc
     2281 ttcgcgcagt acctccacag ggcgtgtcag gtactggatc gaaaccgcca ggatgacgcg
     2341 gtcgaacgcg ctgtccgggt acggcagctc cggtgcggcg ttgagatcgt gcaccacatg
     2401 gtcgtcgagc ctcgggttgg ctgccagctc cgcctcgttc atgccgagac cggcaacgcg
     2461 cgcgtagctg cactccggcg gcaggtggga tacccaggac gacatcaggt ccaggaccgc
     2521 ggcgttctcg gggatgaacg tgcggtagaa ggccgtgagg cagtcgatgg tggcatcgtc
     2581 gatgtgcgtc acgaagcgcg gttcgcgata gaaggccgaa tccggtgact cgtcggcccg
     2641 ggcgaaaagc tgcgggggaa gggcgtcgtc ctgcaaggtg gatccgaacc gttggtgctt
     2701 gggccgccat tctagcgaat gcggtcaatg gcatgttgcc cgcggcctgc cctgtcctcg
     2761 gaggcgagcc cggcctccgt tacccgatga cccggtccca agaccgcgca gataggagct
     2821 gataagattc cgcggtttcg catctcgggg actttcgcgg gaccaccatg gacatgagat
     2881 tcaccgaaga cgaccttgcc tttcaggccg aggtgcgttc gttcatcgat cggcactggc
     2941 ctgccgacgt tcgcctggcc cgggccaccg tgcccgccca cggcctcgat cgccccgccg
     3001 aggagcaggc ctggttcgac gcgctcgtgg cgcgcggttg gagcgttccg aactggccct
     3061 tggagcacgg cggtaccgac tggacgccca cccagcggtt catctgggat cgcgaaaccg
     3121 tgcgggcgga aacgccccag atgagcgtgt tcggcgtaac gatgctggga cccatcctgt
     3181 gccgttgggg aagcgtcgat cagcaggaaa agtttctccc gcccattcgg gaagcccgcg
     3241 tccaatggtg ccagggctac tcggagcccg gagcgggatc ggacctggca agcctcaaga
     3301 cgacggcgcg gctggacggc gatcactaca cggtcgatgg ggagaaaacc tggacgagcg
     3361 gcgcgcacgt ggcggactgg atgttctgcc ttgctcggac ctcgtcgacg aacaagcccc
     3421 aggagggaat cagcttcctg ctcatcgaca tgcgctcgcc gggtgtcgag gttcatccca
     3481 tcgagatcct cggcggccag cacagcgtga acagcgtccg gctggacggt gtccgcgtcc
     3541 cggtcgcgca tcgtgtgggc gaggaaaacc agggctggac ctacgccaaa gctcttctga
     3601 gccatgagcg gacaggggtg gcccgggtcg cgacgtctgg agtgcagctc aatcgcgtac
     3661 gggagattgc cgaagggcag gaaccgagca cagacggtct ctggcaagac gcaggcttcc
     3721 agcgtcgcta tcgggaactc gaaatcgagc tgattggcct cgagatgctg gagttgcgca
     3781 ttcttgcaga cctcgaggcg ggccaggtcc cggggccgga gtcgtccatt ctcaagatca
     3841 agggcacgga gatcggccag cgcatcaacg agttgatggt ggaggccctc ggctactacg
     3901 cgctgcccta tcccgatgag ttgctgatcg acaacgaagg cccggtaggt cacgatcacg
     3961 cgctcgccgc gctcaagggt tggctgttca gccgcgcctg gagcatttac ggcgggtcga
     4021 acgaagttca gaaaaacatc atcgcccggc gggcgctggg tctttgaggg gaagttatgg
     4081 atttttcttt ttccgacgag cagacgctgc tgcaggacag catcgagaag ttcatccaga
     4141 acgactacgc gttcgacgcg cgccagcggg tggttcggga ggagcagggt ttctccagcg
     4201 acaactggcg gacgtttgcc gagctcggct ggctcggggt cccgttcccg gaagccgacg
     4261 gcggcttcgg cggaacggcc atcgagtcga tgatcatgtc ggagcagttc ggcaaagggc
     4321 tggtcgtgga gcccttcctg gcgacggtgg ttctcgccgg gggcgcgctg aagatcggcg
     4381 gcagcgcgtc gcagaaggag aaatacctcg ccggcgtcat ggacggatcg ctgcagggtg
     4441 ccctggcgta cgcggagccc cagggacgat tcaaccttgc cgatctcacc accacggcgt
     4501 ccccggaggg tgacggctac gtgatcaacg gttacaaagc cgtggttatg aacggcccgt
     4561 ccgccgactt tctggtcgta tcctgccgca cgggcggcgg ccagcgggat gcggatggcg
     4621 tcagcctctt cgtgctgcct gcggataccg aaggcgtggc caggcgcgac taccccacgg
     4681 tagatgcctt ccgggcgtcc gaggtgacgc tggagaacgt ccgggtggga agcgatgcgt
     4741 tgatcggcga ggaaggaaag gggctcgcca ttctcgagca ggtcgtcgac gaggccatcc
     4801 tggccgtcgg cgcggaagcc gtgggctgca tggaggtgct ctacaagacg acggtggagt
     4861 actgcaaaac ccgcgagcag ttcggtcagc ccatcggcaa gtttcaggtg ctgcagcatc
     4921 gcatggtgga catgttcatg gagcacgagc aggccaagtc tctgatgtac atggcggcca
     4981 tgcgcctgga cgagggctat ggcccggaag cgcagaaagc cgtatcggcg ttcaaggttc
     5041 aggtgggcaa gtcgggcagg ttcgtcggcc agaacgccgt ccagctgcac ggcgggatgg
     5101 gaatgaccga ggagctcaac gtaggccact atttcaagcg cctcaccatg atcgacacgc
     5161 tgttcggcaa cgtggacttc cacctcaagc ggttcgggtc gctttagatc gtggctcgct
     5221 gggatcgtga ctccggggcc tccatacccg actggttctt cgaggcggtg gagaccgagt
     5281 acgcgacccg gtcagtcgag gtcgatgagt gcgacgtggt gtaccagcta tggggcgacg
     5341 aggataaacc cgggatcctg ctgatccacg gcatgaacgc ccactcccac tggtgggact
     5401 tcatcgcgcc tcagctgctc gacggctatc gggtcgccgc catgaacctc acgggcatgg
     5461 gcgactccga ctaccgctac gagtacgacg gcgccaccta cgcccaggag atcagggctg
     5521 tctgtgacca tgcggggttc gacgagcgtg cagcccaggt ggcgcacagc ttcggcggtt
     5581 acatggccgt caaagcggcc gtgctctttc ccgatcgttt cggggcactg gtcctggtgg
     5641 attccggaat ccgtcatccg gaggagccgg ttccggacag gccggcgatg ggagggcggt
     5701 caaaggtgta tcccgaccgg gagaccgcgc tcggccgctt tcgcgtgcag cccccgcaga
     5761 gctgcgacaa cgcctatatt ctcgagtaca tcgcacgtca ttcgctcatg cccgtggatg
     5821 gcggatgggc ctggaagttc gacgaggacc tgccgtcgac tctaaaggat gtcgagcgca
     5881 cgccggaaga ctaccggcat ctggacgtgc ccacgggcct catctacgga gcggaaagcg
     5941 agctgttctc ggctcgcacg gaagactaca tgcgcgagct cattgcccag gacttcccgg
     6001 ccgtggcgct gccggaagca cagcaccatc tgttcctgga ccagccgctg gcgttcgtcg
     6061 ccgagctgag gaagatgctg gcgacgcttc tcgcctgaac tcgccctagc ccgctcacac
     6121 cggcggcgct tcgtactccg tgtcgtggcc cgggaacgcc tcgtgacctc gcagcatgta
     6181 gtcccagtac atgatcggca gcgccgtctt cttcacccac cacatgcttc ttcgctcctt
     6241 gcgcgggtcc agcgggaagg tcggcgtaac ctttcccccg taggtgaact cggccagcag
     6301 gaccttgccg tacgcggtgg tcagggggca cgagccgtag ccgtcgtaag cttcctcgac
     6361 gctgccgccg tcgagcaggt ggagcagatt gcgcaccacc accggcgcct gcttgcgcac
     6421 ggcagccgcc gtcttggagt ttggggtcga gcaggcgtcc cccagcccga aaacgttggc
     6481 gtaggtggtg tgctgcatgc tgtgctggtg cacgtcgacc cagcctgcct cgttggccag
     6541 ggggctctcc ttcacggccg cgggcgggct ctgatacggg gttgcgtgca gcatgtcgaa
     6601 ggtcatggtg acctcttctc cctctccttc gccgccgacc ttttcgaagg tggcctgccg
     6661 tgcgggcccg tccaccctga tcaggttggt ctgaaaatgg acgtcgatgc cccagcgctc
     6721 ggccaccttg ctcagctccg ctgcgaacag cggcaccgca aacagagcgg ggccgtgggt
     6781 gagaaagtgc agatggcagc ttccgagcag cccgcggcgc tgcagatgat cggcggccag
     6841 gtaggcgatc ttctgaggcg ctcccgggca cttgaacggc agtggcgctt gagtgaacag
     6901 cgcggtggcg ccttcctgaa gtgactgcag gcattgccac gtgtactcga cgtggtcggg
     6961 cgagtagttg ctgcagacgc cgttgctccc cagggtttcc cggagaccgt caattcgctc
     7021 ccagttgagc tccaggcccg ggcagaccac caggtagtcg taggacaacc tcgccccgga
     7081 ggccagggtc accgagttgg actccggatc gaaaccggtt gccgagtccc gatgccacgc
     7141 cacgcctggc gggatcagcc gcgactccgc
//
LOCUS       DBNHQR010000217         4013 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_211776, whole genome shotgun sequence.
ACCESSION   DBNHQR010000217 DBNHQR010000000
VERSION     DBNHQR010000217.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_211776"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..1339)
                     /locus_tag="AC16VR_05030"
     CDS             complement(143..1339)
                     /locus_tag="AC16VR_05030"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6010552.1"
                     /translation="MPALDGLRILDMTQYEAGTACTQALAWLGADVVKIEAPGSGDPG
                     RGVWGETSEDSEYFVVWNSNKRSVAIDLRKPEGRDLLLEMLPHYDVFVENYGPGVIER
                     LNLDYDVMRAIHPEIIYARIKGFGLSGPWAEYKCYDMVGQAAASAFSITGEPDGPPMR
                     PGPTTGDAGTGVQMALAITAAYAQKLRTGEGQFIELSMQEAMTYYLRTATASTNYGER
                     PTPRTGNGDLATMSLYPCAGGGPNDYVFLMAVTPRMWGDMCVAIGREDLIEDPRYKRG
                     ADRAANHASLRAEIGRWTLERDKYEAMRILAEGGVPASAVLDTRDLFDNPHLLDRGFV
                     HTVEHEEHGTIRLLGWPARMSRSQVPIKAAPRLGRDTREVIAADLGLAPESVDALVEA
                     GVVAES"
     gene            1475..2293
                     /locus_tag="AC16VR_05035"
     CDS             1475..2293
                     /locus_tag="AC16VR_05035"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6010553.1"
                     /translation="MSRIFLLISMLAVAACQPPSDRGGADAPSASVAPAPGAEVGALP
                     SLAERLADRPEADRARDAGRMPAEVVEFLGIEEGMTVLDVFSAGGWYAEVLSHAVGPA
                     GTVYAQNTEFLLSMRDGANEKAMAARLADGRLPNVQRLDRELDDLGLAPGSVDAAMLA
                     LNFHDIYNRGGPDGALAFLGRIYSLLKPGGVLGLIDHHGAAGNDNAALHRVEKAKVLE
                     VIDASDFVLEGESDLLVNPDDDMTSMVFAPEIRGRTSRFLLRLRKPADATAVGP"
     gene            2290..3252
                     /locus_tag="AC16VR_05040"
     CDS             2290..3252
                     /locus_tag="AC16VR_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012402.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase domain-containing protein"
                     /protein_id="HIR6010554.1"
                     /translation="MTNPVFPDVRMNIVRAADPVTARVVSNELCMRGKSASFVKHLVL
                     DIGDTPLQGAFRAGQSFGVLAPGEDAQGKPHKVRLYSIACPTWGEDGEARLISTTPKR
                     LIDEFSPQTETDDPDEHHLFLGACSNYLCDLKAGDEVRLTGPNGKRFLLPADPDAHDY
                     LLLATGTGIAPFRGMLLELLTRAQGPCRSRIDLVMGAPYTTDLLYDDLFRRLADEHDN
                     FRYHTAVSREGADGGRGLYVDDLLRRRLDAFRPLLGGDRTLIYVCGLTGMRISLYRLL
                     VEEGLADGYLTVDASLHDVDPGRWSAVDVRRGIKHSDRCLIEVY"
     gene            complement(3278..>4013)
                     /locus_tag="AC16VR_05045"
     CDS             complement(3278..>4013)
                     /locus_tag="AC16VR_05045"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6010555.1"
                     /translation="AIILGGSRGIGWYTARALAVEGCSVALCARDADGVQAAVDALSE
                     DPGGSHFGAAADLTDGDGTRAFVREAITALGGLDLLIHNASGFDRSGDEEGWQRSFQV
                     DVMAGVRAVEEALPALKESDAASITFIGSMASKFYFGRRSSYGPAKAAMRAYANELAQ
                     SFGRDGIRANVISPGAVHFPGGSWDRVKEAQPDFHRNVEKAIPLGRLGTGEEIARIVA
                     FVASPAGIWINSAHIAADGGQVAAVD"
BASE COUNT          611 a         1334 c         1381 g          687 t
ORIGIN      
        1 gagctgatgc ggccgttcgt ggaaaagctg gatcgcgcgg tcatagagga ctgcggccac
       61 tggacccagc acgagaaacc cgaggagctg gccgcgataa tgatcgcctg gctcgaccgc
      121 accttcggcc cggaggttta gcctaggact ctgccaccac gccggcctcg accagggcat
      181 ccaccgactc gggggcaagg cccaggtcgg cggcgatgac ctcccgggtg tccctgccga
      241 gccgcggtgc cgctttgatg ggcacctggc tcctggacat ccttgcgggc cagccgagca
      301 ggcggatggt gccgtgctcc tcgtgctcca cggtgtgcac gaagccgcgg tccaggagat
      361 gggggttgtc gaacaggtcc cgggtgtcga gcacggcgct tgcgggcacg ccgccctcgg
      421 cgaggatgcg catggcctcg tacttgtcgc gctcgagcgt ccacctgccg atctctgcgc
      481 gcagcgatgc gtggttggcc gcacgatccg cgccgcgttt gtagcgggga tcctcgatga
      541 gatcctcgcg gcctatggct acgcacatgt cgccccacat ccttggcgtc accgccatca
      601 ggaagacgta gtcgttgggg cccccgccgg cgcaggggta cagggacatg gttgccagat
      661 cgccgttgcc cgttcgcggg gttgggcgct cgccgtagtt cgtgctggcc gtggccgtgc
      721 gcaggtagta ggtcatggcc tcctgcatgg acagctcgat gaactggcct tctcccgtgc
      781 gcagcttctg cgcgtaggcg gcggtgatgg ccagggccat ctgaacgccg gtacccgcgt
      841 cgcccgtggt ggggcccggc cgcatcggag gaccgtccgg ctcgccggtg atggaaaacg
      901 cgctggcggc cgcctggccc accatgtcgt agcacttgta ctccgcccac gggccgctga
      961 ggccgaaccc cttgatgcgg gcgtagatga tctcggggtg aatggcgcgc atcacgtcgt
     1021 aatccaggtt caggcgctcg atcacgccgg gaccgtagtt ttcgacgaac acgtcgtaat
     1081 ggggcagcat ctcgagcagc aggtcgcggc cctcgggttt gcgcaggtcg atggcgacgc
     1141 tgcgcttgtt gctgttccac accacgaagt actcggaatc ttccgaggtt tctccccaga
     1201 cgccgcggcc ggggtcgccg gagcctggcg cctcgatctt caccacgtcg gcgcccagcc
     1261 aggcgagcgc ctgggtgcag gcagtgcccg cctcgtactg ggtcatgtcc agaattcgca
     1321 aaccgtccag agccggcatg ggctttcccc ttgtgtgttc cccaccgaca agcatatcgg
     1381 tggtcagcac cgaaatgaag aaagcggccg tcgggctaca gggggagccc gcagcgcgct
     1441 atgctgggtc gcactgaggc agagggagga gttcatgagc cgcatcttct tgctgatttc
     1501 aatgctggcc gtggcagcgt gccagccgcc atcggatcgc ggcggcgcag atgcaccttc
     1561 ggcgtccgtt gcgcccgctc ccggcgccga ggtcggcgcg ttaccgtctc tcgcggagcg
     1621 tctggccgac cggccggagg ccgatcgcgc ccgggatgcc ggacgcatgc cggcggaggt
     1681 ggtcgagttt ctcggcatcg aggaaggcat gacggtgctc gacgtgttct ccgccggagg
     1741 ctggtatgcc gaggtgctgt ctcacgccgt cgggccggca ggcacggtat atgcccagaa
     1801 caccgagttt ttgctcagca tgcgcgacgg tgcgaacgaa aaagccatgg cggcgcggtt
     1861 ggccgacggc cggctgccca acgtgcagcg gctcgaccgg gagctggacg accttgggct
     1921 cgcgcccggg agcgtggatg cggccatgct ggcgctgaac tttcacgaca tctacaaccg
     1981 cggcggcccg gacggggcgc tcgccttcct cggacggatc tactcgctgc tgaagcccgg
     2041 cggggtgctc gggctcatcg accaccacgg cgcggccggc aacgacaacg ccgcgctgca
     2101 ccgggtggag aaggcgaagg tgctggaggt catcgacgcc agcgatttcg tcctggaagg
     2161 cgagagcgac ctcctggtca acccggacga cgacatgacc agcatggtct tcgctccgga
     2221 gattcgcggc cgcaccagcc gtttcctgct gcggctgcgc aagccggctg atgcgacggc
     2281 ggtcggccca tgaccaaccc tgtctttccg gacgtgcgga tgaacatcgt tcgggcagca
     2341 gacccggtga cggctcgtgt ggtgtccaac gagctctgca tgcgcggcaa gtcggccagc
     2401 ttcgtcaagc acctggttct ggatatcggc gacacgccgc tgcagggggc gtttcgggcc
     2461 gggcagtcct ttggcgtgct cgcgcccggt gaggacgccc agggcaagcc ccataaggtg
     2521 aggctctact ccattgcctg cccgacctgg ggcgaggacg gtgaagcccg cctgatatcg
     2581 acgacgccga agcggctcat cgacgaattc tcgccccaga ccgaaacgga cgatccggac
     2641 gaacatcatc tgtttctcgg cgcgtgcagc aattacctct gcgacctcaa agccggcgac
     2701 gaggtgcgcc tgacggggcc caacggcaag cgcttcctgc tgccggcgga tcctgacgcg
     2761 cacgattacc tgctgctcgc aacgggcacg ggcatcgccc cctttcgcgg gatgttgctg
     2821 gagttgctga cccgcgctca gggaccctgc cggtcgcgca tcgatctggt catgggggcg
     2881 ccgtacacga ccgatctgct ctacgacgat ttgtttcgcc gcttagcgga cgagcacgac
     2941 aatttccgct atcacacggc ggtaagccgg gagggcgccg atggcggtcg gggtctctac
     3001 gtggacgacc tgctgcggcg gcgtctggac gcgttccggc ccctgctggg cggggatcgc
     3061 accctgattt acgtgtgcgg gctgaccggt atgcgcatca gcctctaccg cttgctggtg
     3121 gaggaaggtc tggcagacgg ctatctcacc gtggacgctt ctctgcacga cgtcgatccc
     3181 ggccgctggt cagcggtgga cgttcgccgg ggcatcaagc actcggatcg ctgcctcatc
     3241 gaggtctatt gacggcggaa ggcgcatccc acggggctca gtccacggcg gccacctgac
     3301 caccgtcggc ggcgatgtgg gccgagttga tccagatccc ggcggggctc gccacgaagg
     3361 cgacgatccg ggcgatctcc tcgccggtgc ctagccttcc cagcgggatg gccttctcca
     3421 cgttgcggtg gaagtccggt tgtgcctcct ttacccggtc ccacgagccg ccgggaaagt
     3481 gcacggcgcc gggcgagatg acgttggcgc ggatcccgtc ccggccgaag ctctgggcca
     3541 gctcgttggc ataggcgcgc atggcggcct tggcgggccc gtaggacgag cgccggccga
     3601 aatagaactt gctggccatg gagccgatga aggtgatgct ggcagcgtcg ctttccttga
     3661 gcgcgggcag tgcttcctcc accgcccgca cgccggccat cacgtccacc tgaaagctgc
     3721 gctgccagcc ctcctcatcg ccgctgcggt cgaagccgct ggcgttgtgg atcagcaggt
     3781 ccagcccacc gagggccgtg atggcttcgc gcacgaaggc ccgggtcccg tccccgtcgg
     3841 tcaggtccgc cgccgcgccg aagtgggatc cgccaggatc ttcggaaagg gcgtccaccg
     3901 cggcctgcac gccgtcggca tcgcgcgcgc agagggccac cgagcagccc tcgaccgcca
     3961 gcgccctcgc cgtgtaccag ccgatgccgc ggcttccgcc cagaatgatg gcg
//
LOCUS       DBNHQR010000218         2210 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_274976, whole genome shotgun sequence.
ACCESSION   DBNHQR010000218 DBNHQR010000000
VERSION     DBNHQR010000218.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_274976"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..443)
                     /locus_tag="AC16VR_05050"
     CDS             complement(102..443)
                     /locus_tag="AC16VR_05050"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HIR6010556.1"
                     /translation="MLLRSLRVARQAFEEGTGWKLTSEGACKGEVCIPLSKPEGTVVD
                     VAELAETLNMPLVGEPERNLWALGPEAIGGRALTTALAPELTLPDLDGNEFTLSSLRG
                     QKILVYAWAPY"
     gene            575..2158
                     /locus_tag="AC16VR_05055"
     CDS             575..2158
                     /locus_tag="AC16VR_05055"
                     /inference="COORDINATES: protein motif:HMM:NF012787.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR6010557.1"
                     /translation="MSALPAFLLQDEEGNAHTFPGSRGALVCFVKEDCPTCNEVMPVL
                     DALNRSVDGALDMLVAGQTREGNRRLKEQHGPGFPILDDSTLKVSFAFDIDTVPTLFL
                     CDAQGVPERTLVGFVRDEWRELAELLADRAGASAPSIDWDALPEWRPGCGSLSVDPVI
                     ADRLRAEAENGPLRARRIEIASQDDEFEFMFDQGFSDGLPLIPPTPERVVRMLSGTSR
                     DPQEVVGVVAPNLGEATVEKVAINAVMAGCRPEYMPVVLAAMEAVCTDEYNIHGVMAT
                     TMGASPVMVVNGPVRHRLGMNMGLGALGQGSRANATIGRAVRLAIRNIGGARPGGTER
                     STLGNPMKFTMCFAEWEERNPWSPLHVERGFAPEDSVVTVFTMTSGPTLMVDQDSRSA
                     AQLAGSLGLCLEAAFHPKAHYATEVLVVLCPEHVDTLLRDGFDKAAIRSRIQEVTARP
                     IAELAEDPVSAVGFKKAAVDAMTPEQRARLLSKFRRDEDIHLVVAGSDAGKFSGAFHG
                     WVTGEIGSISVSRKIEEAG"
BASE COUNT          370 a          704 c          743 g          393 t
ORIGIN      
        1 tcgccgccca gggtgtccag ccctacggtc acgagctcca gaccttgcgg atgcagctca
       61 tcgcgcagta cccgccacac gggcaggtcc gcagagcagc ctcagtaggg ggcccaggcg
      121 tagacgagga ttttctggcc acgcagcgac gacagcgtga actcgttgcc gtcgaggtcg
      181 ggcagggtca gctcgggtgc cagcgccgtg gtcagcgctc ggcctccgat cgcctcaggt
      241 ccgagcgccc acagattccg ctccggttcc cccaccaggg gcatgttcag ggtctcggcg
      301 agctcggcca catcgacaac ggtgccctcc ggtttcgaca ggggtatgca gacctcccct
      361 ttgcaggccc cctctgacgt tagcttccag ccggtgcctt cctcgaacgc ctggcgcgca
      421 acgcgcaatg atctcagcag cacggatgtc gtcctccttt tcggtgatcc gatggtgccg
      481 tctgccgggc ggtggcgcaa gaacgcggcg aacggctttc tcgaatgatc gatctgcggt
      541 aacttctcgg ctccgatcac gccaagagcg agcaatgagc gcgcttcccg cctttctgct
      601 gcaggacgaa gagggcaatg cccacacgtt tcccgggtcg cggggtgctc tggtctgctt
      661 cgtcaaagag gactgcccga cctgcaacga ggtgatgccc gtgctggacg cgctcaaccg
      721 gtcggtggac ggcgcgctcg acatgctcgt ggcaggtcag acccgggagg gcaaccgccg
      781 attgaaggag cagcacgggc ccggctttcc catcctcgat gactcgaccc tcaaagtctc
      841 ttttgccttc gatatcgata cggtgcccac gctcttcctg tgtgacgccc agggcgttcc
      901 cgagcgcacg ctggtgggtt tcgtgcggga cgagtggcgg gaactcgccg agctgcttgc
      961 cgatcgggcc ggggcctcgg cgccgagcat cgactgggat gcgctgccgg aatggaggcc
     1021 gggctgcggt tcgctttcgg tggacccggt gattgccgat cgtcttcgcg ccgaggcgga
     1081 gaacggcccc ttgagagcgc ggcgcataga gatcgcaagc caggatgacg agttcgaatt
     1141 catgttcgac caggggttca gcgatggcct gcccctgata ccaccgacgc cggagcgagt
     1201 cgttcgaatg ctttccggca cctcccgtga tccgcaggag gtggtgggtg tggtggcgcc
     1261 caacctcggc gaggccacgg tggaaaaggt ggccatcaac gcggtcatgg cgggctgccg
     1321 ccccgagtac atgcccgtgg tgctggcagc catggaggcc gtctgcaccg acgagtacaa
     1381 cattcacggc gtcatggcca cgaccatggg tgcgagccct gtgatggtgg tcaacgggcc
     1441 cgtccggcac cgcctgggaa tgaacatggg gctcggtgcc ctggggcagg gctcgcgggc
     1501 caacgccacc atcggccggg cggtgcgcct ggctatccgc aacatcggcg gagcgaggcc
     1561 cggaggcacg gaacggtcga cgctcggcaa ccccatgaag ttcaccatgt gcttcgccga
     1621 atgggaggag cgcaatccct ggtcgccgct tcacgtggag cggggattcg ctcccgaaga
     1681 ctccgtggtg acggtgttca caatgaccag cggtcccacg ctcatggtcg atcaggacag
     1741 ccgcagcgcg gctcagcttg cgggcagcct gggcctttgt ctggaggccg cctttcaccc
     1801 caaagcccac tacgccaccg aggtgctggt ggtgctctgc ccggaacacg tcgatacgct
     1861 gctgcgcgac ggcttcgaca aggcggcgat ccgcagccgg attcaggaag tgaccgcccg
     1921 gcccatcgcg gagctggcgg aagatccggt ttccgcggtt gggttcaaga aagcggccgt
     1981 cgacgccatg acgccggagc agagggcgag gttgctgtcg aaattcaggc gggatgagga
     2041 cattcatctc gtcgtcgccg gctctgatgc cggtaagttc tccggggcct ttcatggctg
     2101 ggttactgga gagattggtt ccatttccgt atcccggaaa atagaggagg caggttgaat
     2161 gaccatcatc atggatccca ccgacgagcg cgtgcccgtc gcccggcagc
//
LOCUS       DBNHQR010000219         4889 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_972220, whole genome shotgun sequence.
ACCESSION   DBNHQR010000219 DBNHQR010000000
VERSION     DBNHQR010000219.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4889)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4889)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4889
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_972220"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="AC16VR_05060"
     CDS             complement(<1..712)
                     /locus_tag="AC16VR_05060"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6010558.1"
                     /translation="MNGLGDWIRCNADWAGGRPALRLGETAYTYAELDAAVDRMAFLL
                     RDGLGIRRGDVVAFLGLNSEQMIVLLFACARNAASLLPLNNRLAGPEHEWILRHSGAR
                     FLFVEDEFAAQANAIDVPGLERVAMGAPPEPHWHEFQRMLGDAREGGFEDVGTLDDRV
                     LLIYTSGTTGQPKGVAHDQRALFYNALNAIHAQEMRASDHVLTVLPLFHAGGLNIQTT
                     PAFYIGAGVSLMRRFDPGE"
     gene            complement(717..1907)
                     /locus_tag="AC16VR_05065"
     CDS             complement(717..1907)
                     /locus_tag="AC16VR_05065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6010559.1"
                     /translation="MTKTEELIGIARELGRDRFLPRRRTYDEEARFPDENYEDLREAG
                     FLGLAIDRRYGGLGADFGTYASISVELGRWCGATALTFNMHTSTMLWSGQLADDLPMS
                     DAERSAHESRRAEIYAGVIEEGHIFAQPFSEPNSAAAAGKAAFDTSAVKVDGGWLVNG
                     RKHFASLSGSAHFYGVLCTDLTDGREPDVRNTLLLAVPASASGFSVAGDWDVLGMRGT
                     VSRSLVLEDVFVPDSGQLLPQGCYYQAALGWPHMFMTLCPTYLGIAEAAFDFTCQYLR
                     GEVEGGPPAGGARDNPVKQVGVAEMRIKLEQARALFEKAIGEAAYRPSKDERLRAYAA
                     QYTVMEFANDICRQAIRVCGGRSLFKSLRLEQLYRDSRCGSLMLPWTPDICIERLGRE
                     SLES"
     gene            complement(1907..2482)
                     /locus_tag="AC16VR_05070"
     CDS             complement(1907..2482)
                     /locus_tag="AC16VR_05070"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6010560.1"
                     /translation="MSVPGFKEAGSGEPVVFLHGIGGNRHAFDAQLAEPGRVRSLVLA
                     QTMARFGKPGSDWQQQFLDARLKPLEDGRAPADFARELITGMFGDPSRAEAIERAVAT
                     MSPLPADVYRQVLTCLVGFDQRGTLPEIDAPTLCLAAELDQTAPPRTMEKMATEIPAG
                     RYDCLQDAGHLAYVEGPAAFTRAVNEFLGQL"
     gene            complement(2479..3525)
                     /locus_tag="AC16VR_05075"
     CDS             complement(2479..3525)
                     /locus_tag="AC16VR_05075"
                     /inference="COORDINATES: protein motif:HMM:NF053113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-dihydroxypyridine 5,6-dioxygenase NicX
                     family protein"
                     /protein_id="HIR6010561.1"
                     /translation="MLQERIEGKWIDCFARVFERCAVAENEEVAILSESQSRPANAQL
                     AELALLRLGARPYHLVIPTPPQKAPVPVRSTGATDVIGHSRSVVAALSSAGFVVDCTV
                     EGMLHAPELPEILKGGARVLMISNEHPEALERLEPDEALETRVKAGIRKLRGASQMKV
                     TSAAGTDLTVSLEGSVAGGGWGYVARPGIMSHWPGGLCLCFPAAGSVSGVLVLDRGDV
                     NLTFKRYLENPVALSIDADYVTEIDGAGVDAELMRSYFAAWGDQEAYAASHLGWGMNN
                     AARWDAMTMYDRNDFNGTELRAFGGNFLYSTGANEVAGRHTLGHFDLPLRNCSVHLDG
                     EAVVVDGALQRELA"
     gene            3656..3901
                     /locus_tag="AC16VR_05080"
     CDS             3656..3901
                     /locus_tag="AC16VR_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010562.1"
                     /translation="MSLIRYPVVQYAYVVDDIEAAGYPAVTTLTSAARVAYMDTRPIL
                     GCFVELYEDNAPLRETFAHWKAVHDAWNGVSDPIRDA"
     gene            3894..4487
                     /locus_tag="AC16VR_05085"
     CDS             3894..4487
                     /locus_tag="AC16VR_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6010563.1"
                     /translation="MPDPEQPGELPFRPADAILRPESFLPAADGTEVCAFLNPYDARG
                     AASAHSAGEAISMAAGRIGPGVRSAIHVHPALTQITYVTAGTLTARMRDPGESEPSRL
                     EVPTGAAVLTEAGTLLQLCNETDRTVEVLYVSTPAYVLETAADGAIRYEDALILGADW
                     DAPAVAHWNVETATQARSRATARRAEAAGRIAGRTRR"
     gene            complement(4506..>4889)
                     /locus_tag="AC16VR_05090"
     CDS             complement(4506..>4889)
                     /locus_tag="AC16VR_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_259446992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HIR6010564.1"
                     /translation="GLPYGRRTHTFNSRLAQELGKWADSRPGGEAIHDALFRAYFVDG
                     LNIGDAGVLVDIAKSAGLDPGGAREVIDQRTFSNAVDEDWKRSRRYGITGVPTFVAGG
                     YGVVGAQPYETLEHLVQRALTESRA"
BASE COUNT          849 a         1656 c         1637 g          747 t
ORIGIN      
        1 cctcacccgg atcgaagcgc cgcatcaggc tcacgccggc cccaatgtaa aaagccggcg
       61 tggtctgaat gttgagacca cctgcgtgga agagcggcag gacggtgagc acgtggtccg
      121 acgcccgcat ctcctgagcg tgaatcgcgt tcagcgcgtt gtagaaaagc gcacgctggt
      181 cgtgcgccac gcccttcggc tggccggtgg tgccggaggt gtaaatcagc agcacccgat
      241 catccagcgt tcccacgtct tcgaaaccgc cttcccttgc gtcaccgagc atgcgctgaa
      301 actcgtgcca atgtggctcc ggcggcgcac ccatggccac ccgctccagg cccggcacat
      361 cgatcgcgtt cgcctgggca gcaaactcgt cttcaacgaa gagaaagcgc gcgccgctgt
      421 gacgcagtat ccactcgtgt tccgggccgg ccaggcgatt gttcaggggg agcaggctgg
      481 ccgcgttgcg ggcgcacgcg aacagcaaaa cgatcatctg ctcgctgttg aggccgagaa
      541 acgccaccac gtcgccgcgc cggatgccca ggccgtcgcg cagcagaaag gccatgcgat
      601 ccacggcggc gtcgagctca gcgtaggtgt atgcagtctc gcccagacgg agcgccgggc
      661 ggccgcctgc ccagtcagcg ttgcagcgga tccagtcgcc gagaccgttc aacgcctcag
      721 ctctccagac tttcccgccc gaggcgttcg atgcagatgt cgggcgtcca ggggagcatg
      781 agcgagccgc agcgggaatc ccggtaaagc tgctccagcc gcagggattt gaacagcgag
      841 cgcccgccgc acacgcgaat ggcctgccgg cagatgtcgt tggcaaactc catgaccgtg
      901 tactgagcgg cgtaagcgcg cagccgctcg tctttgctcg gtcggtaggc ggcctccccg
      961 atggctttct cgaagagcgc ccgggcctgc tccagcttga tgcgcatctc ggcgacgccc
     1021 acctgcttga ccgggttgtc gcgggcgccg cccgccggcg ggccgccttc cacctccccg
     1081 cgcaggtact ggcaggtgaa atcgaaggcg gcttcagcga tgccgaggta ggtggggcag
     1141 agggtcatga acatgtgcgg ccagcccagc gccgcctggt agtagcagcc ctggggcaga
     1201 agctggcccg aatccggcac gaagacgtcc tcgagaacga ggctgcggga gaccgtgccg
     1261 cgcatgccga gcacgtccca gtcaccggca acggagaacc cgctggcgct ggcaggcacc
     1321 gccagcagca gcgtgttgcg gacgtccggc tcgcggccgt cggtgaggtc ggtgcagagc
     1381 acgccgtaga agtgcgccga gccggaaagg gacgcaaagt gcttgcggcc gttcaccagc
     1441 cagccaccgt ccaccttcac ggcggatgta tcgaaggccg ccttgccggc cgcggcggcg
     1501 ctgttgggct ccgagaaggg ctgcgcgaag atgtggcctt cctcgatcac cccggcatag
     1561 atttccgctc ggcgcgactc gtgggccgag cgctcggcgt cgctcatggg caggtcgtcg
     1621 gccagctgac ccgaccagag catggtggaa gtgtgcatgt tgaaggtgag cgccgtggcg
     1681 ccgcaccagc gcccgagctc aacggaaatg ctggcgtagg tgccgaaatc ggcgccgaga
     1741 cccccgtagc gccggtcgat ggcgagaccg aggaaaccgg cttcgcgcag gtcctcgtag
     1801 ttctcgtccg ggaatcgcgc ttcctcgtca taggtccgcc ggcgggggag aaagcggtcc
     1861 ctgcccagct cgcgggcaat accgatcagt tcctcagtct tcgtcatcac agctgaccca
     1921 gaaattcgtt cacggcccgg gtgaaagcag cgggcccttc cacgtacgca agatgacccg
     1981 cgtcctgcag acaatcgtaa cggccggccg ggatctcggt cgccatcttt tccatggtgc
     2041 gcggcggcgc cgtctgatcg agctccgctg ccaggcacag cgtcggggcg tcgatctcag
     2101 gaagggtacc ccgctgatcg aagccgacca ggcaagtgag cacctgccgg taaacgtccg
     2161 cgggcagagg gctcatggtg gcaacggccc gctcgatggc ttccgcacgg gacggatcgc
     2221 cgaacatgcc ggtgatcagt tctctggcaa agtccgccgg ggcacggcca tcctcgagcg
     2281 gcttgagccg ggcatccaga aactgctgct gccaatcgct gcccggcttg ccgaagcgcg
     2341 ccatcgtctg ggcgaggacg agagaacgca cgcgccctgg ctcggccagc tgggcgtcga
     2401 aggcatgccg gttgccgccg atgccatgca gaaacaccac cggctcgccg gaacccgctt
     2461 ccttgaaacc tggaacgctc aagcaagctc gcgctgcaag gcaccgtcca caaccacggc
     2521 ctcgccgtcc aggtgaacgg agcagttgcg cagcggcagg tcgaagtggc cgagggtatg
     2581 gcgcccggcg acttcgttgg cgccggtgga gtataggaaa ttgccgccga acgctcgcaa
     2641 ctccgtgccg ttgaagtcgt tgcgatcgta catggtcatg gcgtcccagc gggcggcgtt
     2701 gttcatgccc caaccgagat ggctggcggc gtaggcttcc tggtctcccc acgccgcgaa
     2761 gtagctgcgc atcagctcgg cgtccacccc cgccccgtcg atctccgtga cgtagtcggc
     2821 gtcgatgctg agcgccacgg ggttctccag gtaacgcttg aacgtcaggt taacgtcacc
     2881 gcggtcgaga acgagaacgc cgctcaccga gccggccgcg gggaaacaca ggcaaagccc
     2941 gccgggccag tgactcatga tgccggggcg cgcaacgtag ccccagccgc cgccggccac
     3001 ggagccttcg agagagacgg tgaggtccgt ccccgcggcc gaggtcacct tcatctggct
     3061 cgcgccgcgc agcttcctga taccggcctt gacccgggtc tccagggcct cgtcgggctc
     3121 cagccgttcc agggcctccg ggtgctcgtt ggaaatcatc agcacccggg cgccgccctt
     3181 gaggatttcc ggcagctcgg gcgcgtgaag catgccctcg acggtgcagt cgacgacgaa
     3241 tcccgccgac gacagcgcgg cgacgacgga acgcgagtgg ccaatcacgt cggttgcgcc
     3301 cgtggaacga acgggcaccg gcgccttctg tggcggcgta gggatcacca gatggtaggg
     3361 cctggccccg agcctgagca gggcgagctc ggcgagctgt gcgttggccg gccggctctg
     3421 gctctcagag aggatggcga cctcctcgtt ctcagcaacg gcgcaccgct cgaacacccg
     3481 tgcgaagcag tcgatccact tgccctcgat acgttcctgc agcattcgac gtttccccaa
     3541 aatgccgcat tgtacagggg ttgccggcgt ccgcagcggt cccggcagcc gcccctgtgg
     3601 gtaacgggtg gggggtattg gggtataaag acaccacccg tgcgaggaga gagcaatgag
     3661 cctgatacgc tacccggtgg ttcagtacgc gtacgtcgtc gacgacatcg aagccgccgg
     3721 gtatcccgcc gtgaccaccc tgacgtccgc cgcccgggtg gcctacatgg acactcggcc
     3781 catcctgggc tgcttcgtgg agctctacga ggacaacgcc ccgctgcggg agaccttcgc
     3841 ccactggaag gccgtccacg acgcctggaa cggcgtcagc gacccgattc gcgatgcctg
     3901 accctgaaca gcccggcgag ctgcccttcc ggccagcgga cgcgatcctt cgacccgagt
     3961 catttctgcc ggccgctgac ggcacggaag tctgcgcttt cctcaatccc tacgacgcgc
     4021 gcggcgctgc cagcgcgcac tcggcgggcg aagcgatcag catggccgcc gggcggatag
     4081 ggccaggtgt gcgttccgcc attcacgtcc atccggcgct gacgcagatc acctacgtca
     4141 ccgcgggcac gctcaccgcg cggatgcggg atccggggga gagcgaaccg tcccgactcg
     4201 aggtgcccac tggagccgcc gtgctgaccg aggcaggcac cctgctgcag ctgtgcaacg
     4261 aaacggaccg gaccgtggaa gtgctctacg tgtccacgcc cgcatacgtt ctggagaccg
     4321 cggctgacgg cgccatccgc tacgaggacg cgttgatcct gggcgcggac tgggacgcgc
     4381 ccgcggtggc ccactggaac gtcgagaccg caacacaggc gcgcagccgg gcgacggcgc
     4441 gacgcgccga ggcggccggg cggatagccg ggcgaaccag gcgctgacgc gccgcgacga
     4501 tccagctaag cccgcgactc ggtgagcgcg cgttgaacca gatgctcgag ggtctcgtag
     4561 ggttgcgcgc cgacgacgcc gtagccaccg gcgacgaacg tcggcacgcc ggtgatcccg
     4621 tatcgccgcg agcgcttcca atcctcgtcc acggcattgg agaaagtccg ctggtcgatc
     4681 acctcccggg caccgccggg atcgagacct gcggacttcg cgatgtcgac gagcacgccg
     4741 gcatcaccga tattcagccc gtcaacgaaa taggcgcgaa acagggcgtc gtgtatggct
     4801 tcgccaccgg ggcgggagtc ggcccacttc ccgagttcct gagcgagccg gctgttgaac
     4861 gtgtgggtac gacggccgta aggaagccc
//
LOCUS       DBNHQR010000220         2634 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_718266, whole genome shotgun sequence.
ACCESSION   DBNHQR010000220 DBNHQR010000000
VERSION     DBNHQR010000220.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_718266"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..1577
                     /locus_tag="AC16VR_05095"
     CDS             186..1577
                     /locus_tag="AC16VR_05095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013714.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR6010565.1"
                     /translation="MTGSSGGVDEAAWDELGSGVRGALLREGDAGYDEARSVWNGMMD
                     RHPAAVLQCLGVSDVMAGVNFARNHGLPLAVKGGGHNVAGKGVCDDGLTLDMSLMRSV
                     RVDPGTLRARAEAGALWRDFDHEAQTFGLTTTGGVVSSTGVAGLTLGGGIGYLTRTHG
                     LACDNLLAADVVTADGRLVQASENENPDLLWALRGGGGNFGVVTSLEFQLHEVGPDVA
                     AAIVFYPLAAGADVLHAYREYAEGAPDAVACYAMVVRGPADLPSEYAGQPVLAIIACY
                     SGAVAEGLRALDPVGSFATPIAATIEGTPYRAMQQMFDPGSPHGVRYYWKSQYLSGLP
                     DEFLETVVRFAREMPGAYTAIGIEPLGGAMERVDPAGTAYPHRRFPFNLGIWSGWDEP
                     SEDSGVISWTREFFEAVVPFGAGAYVNYLSDDEGGRVDEAYGDNYARLRQVKAQWDPG
                     NLFRVNQNIAPRG"
     gene            complement(1652..2473)
                     /locus_tag="AC16VR_05100"
     CDS             complement(1652..2473)
                     /locus_tag="AC16VR_05100"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012995.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase family protein"
                     /protein_id="HIR6010566.1"
                     /translation="MLDDYLDRIGHSGPLNADLATLTALHRAHITAIPYENLEIQLGR
                     ENTLDEKDIFTKLVARRRGGWCYEMNGLLTRALAQIGFQTQRVSGAVHRDEQGSVVEG
                     NHLVGLVELDRTWVVDVGLADGPLEPFPLEPRQWKEGALEFGLERLDSDWWRFHNHAH
                     GLARTFDFTKENRELAHFESMCRFLQTDPASPFVAFALASRRRENGFDALRDTTWFHV
                     EDGRLEKRKIEDEAEYSEVLEQILGNDLGEEAGVLWHRAEERSAAREAAQSATQD"
BASE COUNT          429 a          828 c          896 g          481 t
ORIGIN      
        1 gatgaggacg tcgagccggg ttgccggttc ggcctggctc gttcgctggg cagccacggg
       61 gcgcttctca ctctctgcgg atcccaccca gtctaagacg ttggcgccgg ccgggtccaa
      121 cttgtccgtg gatgtgagga cctctaaagt ccggcaagca gtgcaaccat cgaaaggatg
      181 ctgatatgac gggttccagc ggaggagtcg acgaagcggc ctgggacgag cttgggtcgg
      241 gcgtgcgcgg tgccctgctt cgcgaggggg atgcgggcta cgacgaggcg cgcagcgtct
      301 ggaacggcat gatggatcgg catccggccg ccgttctgca gtgccttggg gtcagcgacg
      361 tcatggccgg cgtgaacttt gcccgaaacc atggcctgcc gctggcagtg aaaggcggtg
      421 gtcacaacgt tgccggtaaa ggcgtctgcg acgatggtct gaccctggac atgtcgctca
      481 tgcgctcggt gcgagtggat cccgggacgc tccgcgcccg cgccgaggcg ggcgcgctgt
      541 ggcgcgactt cgatcacgag gcgcagacgt tcggcctcac caccacgggc ggcgtggtgt
      601 ccagcacggg cgtcgccggt ctgaccttag gtggcggcat cggttacctg acgcgaaccc
      661 atgggctggc ctgcgacaac ctgctcgccg cggacgtcgt cacggccgac ggcaggctcg
      721 tccaggcgag cgagaacgag aatccggacc tgctgtgggc gctgcgcggg ggcgggggca
      781 acttcggcgt ggtcacctcg ctggagtttc agctgcacga ggtaggtccc gatgtcgcgg
      841 cggccatcgt cttctacccg ttagccgctg gtgcggacgt cctgcacgcg tatcgggagt
      901 atgccgaagg ggcgccggat gcggtcgcct gctacgccat ggtggtcaga ggtcccgccg
      961 atcttccctc ggaatatgca gggcagcccg tgttggcgat catcgcgtgc tactccggag
     1021 cggtggctga aggccttcgc gcgctggacc cggtcgggtc attcgccacg cccattgccg
     1081 ccacgatcga agggacgccc tatagagcca tgcagcagat gttcgatccc ggcagccccc
     1141 acggcgtgcg ctattactgg aagtcccagt atctctcggg gttgcctgat gagttcctcg
     1201 agacggtggt gcgtttcgct cgggagatgc ccggcgctta cacggccatc ggcatcgaac
     1261 ccctcggcgg ggcaatggag cgcgtggacc cggcgggcac cgcctacccc catcgccggt
     1321 tcccattcaa tctggggatc tggtcgggct gggatgagcc gtcagaggat tctggcgtca
     1381 tcagctggac cagagagttc ttcgaggcgg tggtgccttt cggtgcgggc gcctacgtca
     1441 actacctcag cgatgatgaa ggtggaaggg tcgatgaggc gtacggggac aactacgcgc
     1501 gcctgcgcca ggtgaaggcg caatgggacc ccggcaacct gttccgggtc aaccagaaca
     1561 tcgcgccccg gggctgatca gctcgagtcg gcggcaggat tttcgtccag gccccggtct
     1621 aaggacctgg cctggtcgcg gacggcggcc atcaatcctg cgtcgcgctc tgggctgcct
     1681 cgcgcgccgc agaccgctcc tcggcccgat gccaaagcac gcccgcttcc tcgcccagat
     1741 cgtttccgag aatctgctcc agcacctcgg agtattccgc ttcgtcctcg atcttgcgct
     1801 tctccaggcg accgtcttcc acgtgaaacc aggtcgtgtc cctcaaggca tcgaacccat
     1861 tctcccgccg ccggctcgcc aacgcgaacg ccacgaacgg ggaggctgga tcggtctgga
     1921 ggaagcggca catgctttcg aagtgggcca gctcacggtt ttcttttgtg aagtcgaagg
     1981 tgcgggcgag cccgtgggcg tgattgtgaa agcgccacca gtccgaatcc agccgctcca
     2041 ggccgaactc gagagcgccc tccttccact ggcgcggttc taaaggaaag ggttccagcg
     2101 ggccgtccgc cagacccacg tccactaccc aggtgcggtc cagctcgacg aggccgacca
     2161 ggtgatttcc ttccacgacg ctgccctgct cgtcgcgatg cacggctccg ctgacgcgct
     2221 gagtctgaaa gccgatctgg gctagcgctc gtgtcagcag accgttcatc tcgtagcacc
     2281 agccgccgcg gcggcgcgcc acgagcttcg tgaaaatgtc tttctcgtcg agcgtgtttt
     2341 cgcggcccag ctgaatttcc agattctcgt agggaatggc agtgatgtgc gcacgatgaa
     2401 gggccgtaag cgtcgcgagg tcggcattca gcgggcctga gtggccgatc cggtcgagat
     2461 agtcgtctag catgtctttc gatgggagcg ccgatgggac agtcaagcta gcatgcggac
     2521 ccggttggaa ccagttccgc tcaaaggggc gccgcagccg aacgggtgcc ctgaccgccg
     2581 gcgctgcaat ccggagggcg attgtactag tatcgagccg tcgccgaggg gaga
//
LOCUS       DBNHQR010000221         1800 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_22112, whole genome shotgun sequence.
ACCESSION   DBNHQR010000221 DBNHQR010000000
VERSION     DBNHQR010000221.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_22112"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..474
                     /locus_tag="AC16VR_05105"
     CDS             223..474
                     /locus_tag="AC16VR_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010567.1"
                     /translation="MNAYDHLVGQHFLRAGVRYTVVKCVGSTAITAYLRDNRIYRVVM
                     RVSDVLDLLEVTEISITVPAMEPKKALPAPQLERASGQS"
     gene            630..980
                     /locus_tag="AC16VR_05110"
     CDS             630..980
                     /locus_tag="AC16VR_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010568.1"
                     /translation="MREQPVFETQGLWVRWSWDEKRKSLKLEKAEPRPQPATAQPTSH
                     WIFTLRDQLVSRSSRLLLRVGVWVLRQRMSSAEVGEYLRETADRVASGHHEPGDPLES
                     HARRCNWNHFFVAK"
     gene            complement(1025..>1800)
                     /locus_tag="AC16VR_05115"
     CDS             complement(1025..>1800)
                     /locus_tag="AC16VR_05115"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018405107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HIR6010569.1"
                     /translation="VVATDDPNVAFKDATHALLVGSRPRSKGMERKDLIEANAAIFSV
                     QGKALNDHADRAVKVLVVGNPANTNCLIAQRNAPDLDPRQFTAMTRLDHNRAAAQLAA
                     RTGKRVTDVAGLCIWGNHSATQYPDLHAATVAGTPAMELVDMAWYTGEFIPTVQQRGA
                     AIIEARGASSAASAANAAIDHMHDWSLGSEGVVSMGVYADGSYGVEPGLIYSFPVICE
                     GGDWQIVQGQEIGEFSREKMAATEKELVEERDAVAHLLS"
BASE COUNT          316 a          612 c          545 g          327 t
ORIGIN      
        1 caacgcaacc atgggcgaga cgaaaaaatt caacacctac atgcacgtca aggatctgca
       61 gacctggcgc atgatcatgg gctgacgcgc ctgccgccga tcggccggtt tccgccattc
      121 gtcatcgccg ctcgccgggc ggccgctaaa ccccgactgc cggtcgtgcg tcccggcgat
      181 ccgcgccgct aagattccgt aggagttgaa gaggaattcg acgtgaacgc ctacgatcat
      241 cttgttggcc agcatttcct gcgcgccgga gtgcgctaca ccgtggtcaa gtgtgtcggc
      301 tcgaccgcca tcaccgccta tcttcgcgac aaccggatct accgggtcgt gatgcgcgtg
      361 agcgacgttc tcgatctcct ggaagtgacg gagatcagca tcaccgttcc cgcgatggag
      421 cccaagaaag ccctgccggc accgcagctc gagcgcgcat ccggccagtc ctgacaaggc
      481 gccgccctgg gtatcccagg ccggaccttt cctcccggtt ccgccaaggg ggcatgagcc
      541 tgccaacgcg gctagtctca aaggccggtt aacgaaccgc ttacatcact gcgatatcag
      601 ttgagctcgg acacctatag attaagccga tgagagagca gccggtattt gagacgcagg
      661 gcctgtgggt ccggtggagc tgggacgaga agcgcaagtc cctgaagctc gagaaggcgg
      721 agccacgtcc tcaaccggcc accgctcaac ctacgtcgca ctggatcttc acgctccgcg
      781 accagctcgt atcgcgcagc agccggctgc tgcttcgcgt cggggtgtgg gtgctcagac
      841 aacggatgtc cagcgccgag gtcggcgagt acctgcgcga aacggccgac cgcgtcgcaa
      901 gcggtcacca cgagcccggc gaccccctgg aaagccacgc ccggcgctgc aactggaacc
      961 acttcttcgt cgccaagtag cggtttgcga aagctgctgc ctccgccagc ctctacaggt
     1021 cgtttcagct cagcaggtgc gctacggcgt cgcgttcctc gacgagttct ttctctgtcg
     1081 cagccatctt ctcacggctg aattctccga tctcctggcc ctgcacgatc tgccagtcgc
     1141 caccctcaca gatgaccggg aaggagtaga tcagccccgg ctcgacgccg tagctgccat
     1201 ccgcgtagac gcccatgctc accacgccct cgctgcccag agaccagtca tgcatgtggt
     1261 cgatggcggc gttcgctgcc gaagccgcgc tggacgctcc gcgggcctct atgatcgcgg
     1321 ccccccgctg ctgcaccgtg ggaatgaact cccccgtgta ccacgccatg tcgacgagct
     1381 ccatggcggg cgtgccggca acggtcgccg cgtgaagatc gggatactgc gtggccgaat
     1441 ggttgcccca gatgcacagc ccggcaacgt ccgtcacccg ttttccggtc cttgccgcca
     1501 gctgcgcggc cgcacggttg tgatccagcc gggtcatggc ggtgaactgc cggggatcca
     1561 ggtcgggcgc gttgcgctga gcgatgagac agttcgtgtt cgccgggttg ccaaccacga
     1621 gcaccttcac cgcgcgatcc gcatggtcgt tgagcgcctt gccctgcacg gagaatatcg
     1681 ccgcgttcgc ttcgatgagg tccttgcgtt ccatcccctt gcttcgcggg cgtgagccga
     1741 ccagcagcgc atgggttgcg tccttgaaag ccacgttggg atcgtccgtg gcgacgaccc
//
LOCUS       DBNHQR010000222        16309 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_845522, whole genome shotgun sequence.
ACCESSION   DBNHQR010000222 DBNHQR010000000
VERSION     DBNHQR010000222.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 16309)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16309)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16309
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_845522"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="AC16VR_05120"
     CDS             <1..801
                     /locus_tag="AC16VR_05120"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR6010570.1"
                     /translation="DGERVEGDVLIGADGIHSLVREQVFRPDSPRASGYVTWRGVVES
                     ETVADLDIPVSAYVDMGPRLSFVYYYVASGRLLNWLALGQANDDKRESWSQTASKSEV
                     LAAFDGWYERPRRLIEATEQPFVTALHDRNPLESWVSGRIAVMGDAAHAMLPYHAQGA
                     VQSIEDAWVLARTLELHGDDPHGALTRFESLRYQRANLLVQQSRNAERWYHLDDPVEV
                     AARNERFRRNNDRYGDDFSPQQHWLYSYDAEAAARGTDEEWRALPTWS"
     gene            complement(811..1368)
                     /locus_tag="AC16VR_05125"
     CDS             complement(811..1368)
                     /locus_tag="AC16VR_05125"
                     /inference="COORDINATES: protein motif:HMM:NF013053.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HIR6010571.1"
                     /translation="MQRLDPKSTAVIVIDVQERLAPAMPEAHLAELLRASRILLRGAA
                     LFGSPVLATEQYPRGLGATVPELGEQLGELNVAPREKLSFSACDDEAFMAELEASGAR
                     AAVVVGMESHVCVYQTVRDLVGAGLDVHVPIDGVCSRRDDHRQAGIDLCVRAGAVRTT
                     TESVAFDWLRVAGSPEFKELSGLIR"
     gene            complement(1389..4772)
                     /locus_tag="AC16VR_05130"
     CDS             complement(1389..4772)
                     /locus_tag="AC16VR_05130"
                     /EC_number="6.3.4.14"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015478961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase family protein"
                     /protein_id="HIR6010572.1"
                     /translation="MEKLLIANRGEIAVRIARAAAELGLVTVAVYAEDDAASLHRSRA
                     DESEALTGSGPAAYLDIGQMVEVACRSGCDAVHPGYGFLSENPDFAAAVEAAGLTFVG
                     PTPETLALLGDKVSARRLAGTCGVPLLAGSQAPVTLEEARAFLAGLPDGVDMVIKAVA
                     GGGGRGMRVVGSAADLEEAFARCCSEARLAFGDDRVYVEEFLPKARHLEVQVLGDGTG
                     AVSHVWERECSIQRRHQKIVEVAPSPTIGPQLRESLLRAAVDIGEAARYRGLGTIEFV
                     VDGSGERYAFIEANARLQVEHTVTEEVTGLDLVRAQLEVAAGRTLAELSLEQRDIPVP
                     RGYAAQARINMETMLADGTTRPGGGTLTSFEVPTGPGLRTDTFGYVGYTTSPAYDSLL
                     AKLIVHSPAGSFADCARKGYRALCETRIEGVATNLDFLRNLFAQPAFQASEVHTGYVD
                     EHVDELVKEAGHRVYHGGRAAAAPATPVLAGVKVDAADPLAVLDHGKSGGASVAAAPR
                     AIALVDEELPAGVSVIRAPMQGTVVSFEVAPGDEVYVGRQVLVMDAMKMEHVVKAEVS
                     GIVQRLGVNEGDTVFEGHPLVYLQEADIAAGEEAGQEAFDLDYVRPDLAEVFERKAAG
                     HDENRPEAVAKRHGRGHRTARENVADLCDPGSFVEYGSVVVAGQRRRRSMEDLIKNTT
                     GDGMVCGLGRVNGDLFGPERSRAMVMSYDYMVLAGTQGIKNHAKKDRLFEIAEAQRLP
                     TVLFAEGGGGRPGDTDGSGVAGLDCWAFTYFARLSGLVPIVGITNGRCFAGNAVLLGC
                     CDVIIATQGSNIGIGGPAMIEGGGLGIFKPEEVGPMDVQVPNGVVDIAVEDEAQAVAV
                     AKKYLSYFQGPVEDWDAPDERKLRFVVPENRLRYYDMREVIAGIADVGSVLELRQGWG
                     PGIITAFIRVEGMPLGVIANNPGHLSGAIDAEGADKGTRFMQLCDAFDIPILSLCDCP
                     GIMVGPESEKTALVRHAARMFVVGANIDVPLMTIVTRKGYGLGAQAMAGGSFKAPLFT
                     VTWPTGEFGGMGLEGAVKLGYRKELQAIDDPAERKDAYEKMVADMYQRGKAVNMASHF
                     ELDEVIDPAESRRWIVAGLRSVPPAPRRSGKKRPNVDTW"
     gene            4949..5653
                     /locus_tag="AC16VR_05135"
     CDS             4949..5653
                     /locus_tag="AC16VR_05135"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008174689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6010573.1"
                     /translation="MTVTLSASGLTKVYQTGDVVVHALRGAELELREGELVVMLGPSG
                     SGKSTLLNILGGLDVPTAGTVWFRNRQLTASDEASLTEYRRRHVGFVFQFYNLIPSLT
                     ARENVALVTEISSNPMDAAEALALVGLAERLDHFPAQLSGGEQQRVAIARAIAKRPEI
                     LLCDEPTGALDSETGILVLAAIDQVNRELGTTTAVITHNTVIGAMADRVMHMADGLIA
                     RVDENPDRQPPDELSW"
     gene            5698..8022
                     /locus_tag="AC16VR_05140"
     CDS             5698..8022
                     /locus_tag="AC16VR_05140"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6010574.1"
                     /translation="MKGQVIAVGLVIASGVAVLVMALSTLEALRETTDAYYERYRLAD
                     VFAGVKRAPEPLSDRIREIEGVQTVQTRIVKFATLDVLHFPEPVMGQFVSLPESGPPL
                     LNQLALRAGRHVEPGQHDEVVVNEPFAEAHGLTPGDSLVAVLNGKRRLLRVTGIALSP
                     EFIYSLGPGALMPDDERFGVIWMNRKALAAAFDLKDAFNNISLQLTRGTRPEGVITQL
                     DRLLEQYGGIGAVARANQMSNWFVMNEIDQLATISRILPSIFLLVSAFLTNMVLTRLI
                     DTERAQIGLMKAFGYTSFEVGFHYTKLVLGMAVVGIVVGLFVGGWFGRLNTQMYAEVF
                     RFPLLLYRPSPSAFAIASALSLLAALAGAMSSVRRATRLPPAQAMQPPAPPTFRRGRL
                     AHSRFGRWLDQPTRIVLRNIARWPGRAALTTTGIAASVALLVLALQWNDSLNYLAQSY
                     FFQAQRQHVMIGLADTRSATVMHDIEQLPGVLAAEPMRIVSADLSRGAVTHRGALTGV
                     ADGAQLQPIYDDARGVEIPTPPEGLVLGITLAEKLGVGVGDRIWVNVLEGRRPNVQLP
                     VVEVVETYIAMPAYIHIDALNRLLKERPSVEYANLRIDRLEESRLYAELKKLPVVSAV
                     MLRQAAIDAFYDNVVEHLMVFITMFSSLACILGFGVAYNSVRIALSERGRELATLRVL
                     GFTRGETSYILLGEVALLIVAALPLGCLLGRLLSQLIAAAFNTELFRVPLTIDPSTYG
                     IAVLIALAATTLSAALVRRRVDRLNMIEVLKTRE"
     gene            8025..9233
                     /locus_tag="AC16VR_05145"
     CDS             8025..9233
                     /locus_tag="AC16VR_05145"
                     /inference="COORDINATES: protein motif:HMM:NF052847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6010575.1"
                     /translation="MNANAKRILVWSVVAIAVAVGLTIAFMPRPVTVDLITVIPAPME
                     VTADEEGETRVHDVFVLSAPVAGRLRRIDAHVGDPVLADETVLARIEPADPTFLDPRT
                     EAQARAARQAAVSALTLAKARVEEARAEFEYADAEFHRARELIAKGTISVRDAEAAER
                     AYKARRAALSTAQAAVQMRTYELQQAEALLISPAQSRTRTDECDCVDVTAPVSGQVLQ
                     IVNPSERIVTAGEALVEIGDPGDLEIVVDFLSADAVQIEPGQRVIIERWGGESALVGH
                     VRRIEPFGFRKTSALGIEEQRVNVIVDFTSPRNEWSRLGHGYQVDVRVVLWEQDAVLA
                     IPLTALFRDDQRWAVFVDDDGRAALREVVVGHRNGMVAEIVSGLARGDRVVLHPSNRV
                     IDGVRITGRG"
     gene            complement(9268..10818)
                     /locus_tag="AC16VR_05150"
     CDS             complement(9268..10818)
                     /locus_tag="AC16VR_05150"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: protein motif:HMM:NF004829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HIR6010576.1"
                     /translation="MSSYDVAIVGMGPVGAAAAILFADAGLKVIAFERDAHVYPLPRA
                     VALDGEVVRGFQRVGRAGELAALLQPVRPGERAGFANSRREWLFGQPYATFGVNGWQP
                     ISMFDQPELEAHLRRLAMDHSGVTGCLEVQVETFEETGGQVAIRARQADGALSETSAR
                     FLVACDGAASGARRALEIGWRDLGYDHDWLVVDVVLKDGHTLGLDTLQVCDPDRLSTY
                     IATKDPFRRWEFKLNPGETREEMLEPERIRELIDPWTPRGTYEIRRSAVYQFHAATAE
                     TWRKGRVLLAGDAAHQTPPFLGQGLNAGMRDVINLAWKLPLVLSGAADEGLLDSYQAE
                     RGAHAADLVEWAVAIGKLMEHLAAVEAAQRVGRKPPEVPDGNRASGYGQGREAPPLRD
                     GVVMVDQVSEHGSTGYLFRQPAVRDADGEEYLLDDRLGAGFALVARSEADLAMSEASR
                     AIVDALGMSTTTLEGLEATRGRFDRLFEGSAAAVVRPDRYVFGHTSQSLSLDALLGEL
                     AARLGLRL"
     gene            complement(10815..11462)
                     /locus_tag="AC16VR_05155"
     CDS             complement(10815..11462)
                     /locus_tag="AC16VR_05155"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6010577.1"
                     /translation="MPNQSHRGPVQERAQVTRDRLLDAAVRCFAAAGYEGSSTRQIEA
                     SAGVKRGLIAYHFGTKEALWKQAVTWWFGQAPHMLRVRVSGQGGSDPAAQLRALITGF
                     VRFSASFPEANRLMIREGMDDDWRLQWLAEHIVGPWYERLGAAFEEARSAGAVRNVPF
                     VHFYYILTGAGSLLFSMAPEAERLAGIDPYDDDVVRRHAEVIADLLIPGSGEHAH"
     gene            11551..11763
                     /locus_tag="AC16VR_05160"
     CDS             11551..11763
                     /locus_tag="AC16VR_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010578.1"
                     /translation="MTDKLYARFRVPDLNRQARSRSSRRRLGSQVFDYWKDPNGFTME
                     HFTDGDLFNASSGSEKWPVDVALGSH"
     gene            11843..12895
                     /locus_tag="AC16VR_05165"
     CDS             11843..12895
                     /locus_tag="AC16VR_05165"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6010579.1"
                     /translation="MLPGGLIFGTFLVLVLVLALVLVLVLVAGWYVWERRQRRTRAMP
                     GGLHEEITLPHRQAWELYHNNLSLCSKKTRACLSELSIDYESHHIDLIETGRYENVSR
                     EFLKVNPAGLVPVLVHEGHPVYESHAQIAFAAARSDDPHRLVPADPDQRRQMDDWVRK
                     TSLIGDDPVGAVHQTAGNAVPGLTLPLFATMLERIPLRRVLEGLLFHRLKRRAFILMI
                     IKLYGIDSLTSAKPLLRVVQASRDAMHRHLDDLEATLKDSGGPWIVGEHFSLADIGMM
                     AILERLREADWLDVLVSGRRPRVAAYWRALQTRPSYQSAIVACRGEAIERGTARLVAA
                     KQNNPVLRFALIETIT"
     gene            12943..13821
                     /locus_tag="AC16VR_05170"
     CDS             12943..13821
                     /locus_tag="AC16VR_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008094299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR6010580.1"
                     /translation="MKLVSFSTTENPKPRLGAVRDGGIADLSGSDGIPETMLGLLHAG
                     DAAMKAAHEAAEAAADTVPLDQVTLHPPVLTPGKILAIGLNYGDHIEESGMGKPKHQV
                     WFNKQHNCVNGPYGDIALPSVSEMLDYEAELVVVIGKRCKHVPRERAAEVIAGYCCGN
                     DVSVRDWQMRAQTMQIGKSFDTHGPTGPWLVTPDEVGDPHDLDIRAEVNGELRQNSNT
                     KHLIYDCYDAVAHLTQAFTLDPGDLLFMGTPAGVGAAMKPPQFLKAGDVVRVEIEKLG
                     YLENRVVPEAAECVIE"
     gene            complement(13823..14422)
                     /locus_tag="AC16VR_05175"
     CDS             complement(13823..14422)
                     /locus_tag="AC16VR_05175"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6010581.1"
                     /translation="MAQAQTHDHEIVSIYPFTDEEVDQLMNNSVECVLMWATKDGWPV
                     GVTHAFVWHDGKIWLTFAAHRHRTVAIRRDNRVSVNVSSCGYPPGASEELPGGAITFK
                     GTGEFFDDEETKKWFYTALSQKLNPNSPEGEQFFYNLLDSPLRTVLAVTPVKKIMYNG
                     KLAGGHMAGTVKEEDLGPRLGVDKERMNAERAKRGLDPR"
     gene            14599..15822
                     /locus_tag="AC16VR_05180"
     CDS             14599..15822
                     /locus_tag="AC16VR_05180"
                     /EC_number="5.4.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR6010582.1"
                     /translation="MSNLRRSTQLYERAKAVIPGGIPGIRAPENFVPGAYPILLAGGR
                     GGHVTDVDGNDYVDLLLGYGPVILGHAEQAVDSAAHERAAHGFCLDLPEPVMIELAEQ
                     LVSMVPSAEQALLLKTGSDATSAAIRIARTHTGRSVVLRCGYHGWHDWCLQGDPGVPS
                     GTTDTVIAFPYNDLDRLAALLTAHSGDVATIMLTPVGHEFDRQIEQPEPGFLEGVRSL
                     ADQHGVVLAFDEVRTGFRVHPGGAQGLYGVIPDLTALGKAMSNGYAVTALVGRRDVMQ
                     SARSTFISSTYFPNGMEMAAALETLKLIAEHDVLTGIAATGAALAGGLSEIVARHGLP
                     VSLSPYDEMPFLHFDPDLASGQPERRDAFYGALARSGVFAHPRHHGFLCWRHSADDVT
                     RVLEAVDAAAAALGS"
     gene            complement(15819..>16309)
                     /locus_tag="AC16VR_05185"
     CDS             complement(15819..>16309)
                     /locus_tag="AC16VR_05185"
                     /inference="COORDINATES: protein motif:HMM:NF013717.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HIR6010583.1"
                     /translation="LASARVQGSASEFPFRLITKREVTTHNSWTHNYEGFLRGDQGRN
                     YVFMNPTDAAALGLKPDDLADVRSSAGAIRLPVRTSDDLLAGVVAVPHGWGHQASGQR
                     VAAGSLGVNVNILASSGAAHVDPFSGMSHLTGLPVDVRLAADDRLAGNWSGIARPPGG
                     PD"
BASE COUNT         2730 a         5637 c         5288 g         2654 t
ORIGIN      
        1 gacggcgagc gcgtcgaggg cgacgtgctc atcggcgcag acggcattca ctccctcgtc
       61 cgggagcagg tcttccggcc ggactcgccg agagcgtccg gatacgtgac ctggcgcggg
      121 gtcgtcgagt ccgagacggt ggccgatctg gacattcccg tgtccgccta cgtggacatg
      181 ggaccgcggc tgtccttcgt ctactactac gtcgcctctg gccggctgct gaactggctc
      241 gccctgggac aggccaacga cgacaagcgc gagtcctggt ctcagaccgc cagcaaatcc
      301 gaggtgctgg ccgctttcga cggctggtac gagcggccca ggcgcctgat agaggctacc
      361 gagcagcctt tcgttaccgc gctgcacgac cgcaacccgc tggagagctg ggtgtccggg
      421 cgcatcgccg tcatgggcga tgccgcccac gccatgctcc cctaccatgc ccagggcgcg
      481 gtgcagtcca tcgaggatgc ctgggtgctg gcccgaaccc tcgagcttca cggcgacgac
      541 ccccacgggg cgcttacgcg gttcgaatcg ctgcgctacc agcgcgccaa cctgctggtg
      601 cagcagtcgc gcaacgccga gcgctggtat cacctggacg atcccgtcga ggtggccgca
      661 cgcaacgagc gtttccgccg gaacaacgat cgctacggtg acgacttctc accgcaacag
      721 cactggttgt acagctacga tgccgaggcc gctgcgcgcg gaaccgacga ggagtggcgc
      781 gcgctgccga cttggtcctg accgggccgc ctaccgaatc agcccggaca gctccttgaa
      841 ctctgggctt cccgccacgc gcagccagtc gaaggcgacc gactcggtgg tcgttctaac
      901 ggctcccgcg cgcacgcaca gatcgatccc cgcctgccga tgatcgtcgc ggcgcgagca
      961 cacgccgtct atgggcacgt gaacgtcgag accggcgcca acaaggtctc gaacggtctg
     1021 gtagacgcag acgtgcgatt ccatgccgac caccaccgcg gcgcgagcgc cgctggcttc
     1081 gagctccgcc atgaaagcct cgtcgtcgca ggcgctgaag ctgagcttct cgcgcggcgc
     1141 cacgttgagc tcgccgagct gctcgccaag ctccgggacg gtggccccca gcccgcgagg
     1201 atactgctcg gtggccagca cgggagagcc gaacagcgcc gcgccgcgaa gcaggatgcg
     1261 gctggcgcgc aggagttccg ccaggtgcgc ctccggcatg gcgggcgcga ggcgctcctg
     1321 aacgtcgatg acgatgacgg cagtggattt cgggtccaga cgctgcatat gctctcccct
     1381 tgcttgggct accaggtgtc tacgttcggc cgtttcttcc cggaccgccg cggtgccgga
     1441 ggcaccgagc gaagtcctgc gacgatccac ctgcgagatt ccgccgggtc gatgacctcg
     1501 tcgagctcga agtgcgaggc catgttcacc gccttgccgc gctggtacat gtcggccacc
     1561 attttctcgt aggcgtcttt gcgctcggcc gggtcgtcga tggcctgcag ctctttgcgg
     1621 tatccgagct tgacggcccc ttcgagaccc atgccgccga actcgcccgt gggccaggtc
     1681 accgtgaaca ggggcgcctt gaagctgccg ccggccatgg cctgagcacc gagcccgtac
     1741 cccttgcggg tgacgatggt catcagcgga acgtcgatgt tcgcgcccac cacgaacatg
     1801 cgggccgcat ggcggacgag ggccgtcttc tcgctctccg gccccaccat gatgcccggg
     1861 caatcgcaca ggctcaggat ggggatgtcg aaggcgtcgc acagctgcat gaagcgggtg
     1921 cccttgtccg cgccctcggc gtcgatggcg ccggacagat gtccgggatt gttggcaatc
     1981 acgccgagcg gcatcccctc cacgcgaatg aacgcggtga tgatgccggg cccccaaccc
     2041 tggcgcagct ccagcaccga gccgacgtcg gcgatgccgg ctatcacctc gcgcatgtcg
     2101 tagtagcgca gccggttctc ggggacgacg aatcgaagct ttctctcgtc cggcgcgtcc
     2161 cagtcttcaa ccggtccctg aaagtacgac aggtacttct tggcaaccgc cacggcttgt
     2221 gcctcgtcct ccaccgcgat gtcgaccacc ccgttgggca cctgcacgtc catgggcccg
     2281 acctcttcgg gcttgaaaat gccgagcccg cccccctcga tcatcgccgg cccgccgatg
     2341 ccgatgttcg aaccctgcgt ggcgatgatc acgtcgcaac agcctagaag cacggcgttg
     2401 ccggcgaagc agcgcccgtt ggtgatgccg acgatgggca ccaggcccga gaggcgggcg
     2461 aagtaagtga aggcccagca atccaggccc gcaacgccgg acccgtcggt gtcgcccggg
     2521 cggccaccac cgccttcggc gaacagcacg gtgggcaggc gctgggcttc ggcgatctcg
     2581 aacagacggt ctttcttcgc gtgattcttg atgccctggg tcccggcaag caccatgtag
     2641 tcgtaggaca tcaccatggc gcgggaccgt tcagggccga acaggtcgcc gttgacccgg
     2701 ccgagcccgc agaccatccc gtcgcccgtg gtgtttttga tcaggtcctc catgctccgg
     2761 cggcgacgct gtccggcgac caccacggat ccgtactcca cgaacgaacc cggatcgcac
     2821 agatcggcga cgttctcccg cgccgttcgg tgtcccctgc cgtggcgttt ggccacagcc
     2881 tccgggcggt tctcgtcgtg cccggccgcc ttccgttcga acacctccgc gaggtccggg
     2941 cgaacgtagt ccagatcgaa cgcttcctgg cccgcctcct cgcccgcggc gatgtctgct
     3001 tcctgcagat agacgagggg gtggccctcg aagaccgtgt cgccttcatt cacgcccagg
     3061 cgctggacga tgccgctgac ttccgccttg accacgtgct ccatcttcat ggcgtccatg
     3121 accaacacct gcctgccgac gtagacctca tcgccgggtg ccacctcgaa gctgacgacg
     3181 gttccctgca tgggcgcgcg gatgaccgaa acccccgcgg gcagctcttc gtccaccagc
     3241 gcgatggccc gcggggccgc tgcgacgctc gccccgccag acttaccgtg atcgagaacg
     3301 gcgagcggat ccgcggcgtc cacctttacg ccggccagca cgggcgtggc cggcgcagcg
     3361 gcagcgcgtc ctccatgata gaccctgtgg cccgcttctt tgacaagctc gtccacgtgc
     3421 tcgtccacgt agcccgtgtg cacctcgctg gcctggaagg cgggctgggc gaagagattg
     3481 cggaggaagt ccagattggt cgccaccccc tcgatgcggg tctcgcagag ggcccgatag
     3541 cctttgcggg cacagtcggc aaagctgcct gcgggggagt gaacgatgag cttggccagg
     3601 agcgagtcat aggccgggct cgtggtgtag ccgacataac cgaaggtgtc cgtacgcaga
     3661 ccggggccgg taggcacctc gaagctcgtc agcgtgccgc caccgggcct ggtggtgccg
     3721 tccgctagca tggtctccat gttgatccgc gcctgggccg cgtagcccct gggcactggg
     3781 atgtcgcgct gctcgaggga gagctcggcc agggtgcggc cggcggcaac ttccagctgg
     3841 gcgcgaacga ggtccaaccc ggtcacttcc tcggtgaccg tgtgctcgac ctgcagccgc
     3901 gcgttggctt caatgaaggc gtagcgctca ccggaaccgt caacgacgaa ctcgatggtg
     3961 ccgaggcccc gataacgtgc ggcctccccg atgtccacgg cggcgcgcag cagcgactcg
     4021 cgcagctgag gaccgatcgt cgggctgggc gccacctcca cgatcttctg atggcgccgc
     4081 tgtatgctgc attcgcgctc ccagacgtgg ctcacggcgc cggtgccgtc gcccagcacc
     4141 tgcacctcca ggtggcgggc tttgggaagg aattcctcta cgtagacgcg atcgtcacca
     4201 aaggcgagcc tggcttccga gcagcagcgc gcgaaagcct cttccagatc ggcagcgctg
     4261 ccgaccaccc gcatgccccg gccaccgccg ccggcgacgg ccttgatcac catgtccacg
     4321 ccgtccggga ggccggcaag gaaggcgcgg gcctcctcga gcgtaaccgg cgcttggctg
     4381 cccgccagca gcggcacgcc gcaggtgccg gcaagccggc gcgcgctcac cttgtcaccg
     4441 agcagcgcga gggtttccgg agtcgggccg acgaaggtga gaccggccgc ctccacggct
     4501 gcggcaaaat cggggttctc gctcaaaaag ccgtagccag gatggacggc gtcgcatccc
     4561 gaccggcacg ccacctccac catctggcca atgtccaggt aggccgcggg gccgctgccc
     4621 gtgagcgctt cggattcgtc cgcccgggag cggtgcaggg aggcagcatc gtcctcggca
     4681 taaacggcaa cggtgaccag gccgagctcg gcagccgcac gggcgatacg aacggctatc
     4741 tcgccccgat tggcaatcag cagtttctcc aagctccctc ctcgaccgtt cccgtgagat
     4801 gggcggccag ctccccgaaa ggcagaaaaa cgatcggcga ttgtctgacg ttgatcagtc
     4861 gaagtccaac gggcaacctc gtgaaagtgc ggatggtggt tctgcccgca gaatcatcta
     4921 atctgggctt cccgacgaag gcgaaaccat gactgtcacc ttgagcgcaa gcggtctcac
     4981 caaggtctac cagaccgggg acgtcgtcgt gcacgcgctg cgcggggccg agctcgagct
     5041 gcgcgaaggt gagctggtgg tgatgctggg cccttcgggg tcggggaagt ccacgctgct
     5101 gaacatcctc ggcggtctgg acgtgcccac ggccgggacg gtctggttcc gcaatcgcca
     5161 gctgacggcc tccgacgagg cctctctcac agagtaccgg cgccggcacg tgggcttcgt
     5221 cttccagttc tacaacctga ttcccagcct gaccgcccgg gaaaacgtcg ccctggtcac
     5281 cgagatctcc tcgaacccca tggacgctgc ggaggccctc gcgctggtcg gcctcgccga
     5341 gcgtctggat cacttcccgg cccagctctc cggaggcgag cagcagcgtg tggccatcgc
     5401 tcgggcgatc gcgaagcgcc ccgagatcct gctctgcgac gagccgaccg gggccctgga
     5461 cagtgaaacc ggcatcctcg tgctggccgc catcgaccag gtcaaccgtg agctcggcac
     5521 caccacggcc gtgatcaccc acaataccgt catcggtgcc atggccgacc gggtcatgca
     5581 catggcggac gggctgatcg cccgcgtcga cgagaatccg gaccgccagc cccccgacga
     5641 gctgagctgg taactgtgcg ggcgctcgat acgaaggtgc tccgcgacct cgtccacatg
     5701 aaaggacagg tcatcgccgt cggcctggtc atcgcatccg gggtggcggt gctggtgatg
     5761 gccctgtcca ccctcgaggc ccttcgcgag accaccgacg cctactacga gcgttaccgc
     5821 ctggcggacg tgttcgccgg ggtcaaacgg gcgccggagc ctttgtcaga caggattcgc
     5881 gagatcgaag gcgtgcagac ggtgcagacc cgcatcgtca agttcgcaac gctggacgtc
     5941 ctccacttcc cggaaccggt gatgggccag ttcgtttcct tacccgaaag cggcccgccg
     6001 ctgctgaacc agctcgcgct gcgtgccgga aggcatgtcg agccaggcca gcatgacgag
     6061 gtggtcgtca acgagccgtt cgccgaagct catgggctca cgcccggcga ctccctggtg
     6121 gcggtgctga acggaaagcg ccgcttgctc cgcgtgacgg ggatcgctct ctctcccgag
     6181 ttcatctaca gcctggggcc cggcgctctg atgcccgacg acgagcgctt cggcgtgatc
     6241 tggatgaacc gcaaggcgct cgccgccgcc ttcgacctca aggacgcgtt caacaacatc
     6301 tccctgcagc tcacccgcgg aaccaggccg gaaggcgtga tcacgcagct cgatcggctg
     6361 ctcgagcagt acgggggcat cggcgccgtc gcccgcgcca accagatgtc gaactggttc
     6421 gtcatgaacg agatcgacca gctggccacg atatcgcgca ttcttccgag catatttctg
     6481 ctggtctccg ccttcctgac caacatggtg ctcacccggc tgatcgacac ggaaagagcc
     6541 cagatcgggc tgatgaaggc cttcggatac acgagtttcg aggtgggctt tcactacacg
     6601 aagctggtgc tgggcatggc cgtggtgggc atcgtcgtgg gcctgttcgt cggcggctgg
     6661 ttcggccgtc tcaacacgca gatgtacgcc gaagtattcc gcttccccct gctcctctat
     6721 cggccgagcc cgagcgcgtt cgccatcgcg tcggcattga gcctgctggc agcgctcgca
     6781 ggtgccatga gcagcgtccg ccgggcgacc cgtctgcccc cggcccaggc catgcagccg
     6841 ccggcgccac ccacgtttcg acggggacgg ctcgcccact cgcgcttcgg ccgctggctg
     6901 gaccagccaa cccgcatcgt tctgcgcaac atcgcccgtt ggcccggccg cgccgcgttg
     6961 accaccaccg ggatcgccgc ttcggtcgcc ctgctggtcc tcgcgctgca gtggaacgac
     7021 tccctgaact acctggcgca gagctacttc tttcaggccc agcgccagca cgtgatgatc
     7081 gggcttgcgg atacccggtc cgccacggtg atgcacgaca tcgagcagct cccgggggtg
     7141 ctggcagcag agcccatgcg catcgtcagc gcagacctat cccgaggagc ggtaacccac
     7201 cgcggcgcgc tgacgggcgt cgccgacggc gcgcagctgc agccgatcta cgacgacgcg
     7261 cggggcgtgg agattccgac gccgcccgaa ggcctggtgc tgggcatcac cctggcggag
     7321 aagctgggcg tgggcgtggg cgaccggatc tgggtaaacg tgctcgaggg acggcggccc
     7381 aacgttcagc tgcccgtcgt cgaggtggtg gaaacctaca tcgccatgcc ggcctacatc
     7441 cacatcgacg cgctcaaccg cctgctcaag gaacgcccga gcgtggaata cgcgaacctg
     7501 cgcatcgacc gcctcgaaga gtccaggctg tacgccgagc tcaagaagct gcccgtggtc
     7561 tctgcggtga tgctgcgcca ggcggccatc gacgccttct acgacaacgt ggtggagcat
     7621 ctgatggtgt tcatcaccat gttttccagc ctcgcctgca tactcgggtt cggtgtcgcc
     7681 tacaacagcg tgcgtatcgc tctgtcggaa agggggcgcg agctggctac cctgcgggtg
     7741 ctcggcttca cccgagggga gacgtcctac atcctcctgg gcgaggtggc gctgctcatc
     7801 gtggccgccc tgccgctggg ctgcctgctc ggccgactcc tctcccagct gatcgccgcc
     7861 gccttcaata ccgagctgtt ccgcgtgccc ctgaccatcg atccgtccac gtacggcata
     7921 gccgtgctca tcgcgctggc ggccacgacg ctgtccgcgg ccctcgttcg ccgacgcgtc
     7981 gaccgcctga acatgataga agtgctcaag acccgggagt agaaatgaac gccaacgcca
     8041 agcggatact ggtctggtcc gtcgtcgcca tcgccgtggc cgtcggcctg acgatcgcat
     8101 tcatgcccag accggtgacc gtggacctga tcacggtcat tccggcgccc atggaggtca
     8161 ccgccgacga ggaaggtgag acgcgcgttc acgacgtctt cgtgctgtcc gctcccgtcg
     8221 ccggccggct gcgccgcatc gatgcccatg tcggcgaccc ggtccttgcc gacgaaaccg
     8281 tcctggcccg catcgagccg gccgatccga cctttctcga cccgcgcacg gaggcccagg
     8341 cccgcgccgc gcggcaggcg gcagtctctg cgctcaccct ggcaaaggcg cgagtcgagg
     8401 aagcgcgggc agagttcgag tacgccgacg ccgagttcca ccgggcccgg gaactgatcg
     8461 ccaagggcac catctccgtc cgcgacgccg aggcggccga gcgcgcctac aaggcgaggc
     8521 gggccgccct gagcacggcc caggccgcgg tgcagatgcg cacctacgag cttcagcagg
     8581 cagaggcgct gctgatctcg cccgcgcaat cgagaaccag aaccgatgag tgcgactgcg
     8641 tggacgtgac cgcgccggtg agcggccagg tgctgcagat cgtcaacccg agcgagcgga
     8701 tcgtgaccgc aggcgaagcg ctcgtggaga tcggcgaccc gggcgacctg gagatcgtgg
     8761 tggattttct gtccgccgac gcggttcaga tcgagcccgg gcagcgggtc atcatcgagc
     8821 gctggggcgg cgagtccgcc ctggtcggcc acgtgcggcg catcgagccc ttcgggttcc
     8881 gcaagacgtc ggccctgggg atcgaggagc agcgggtgaa cgtgatcgtg gatttcacga
     8941 gcccgcgcaa cgagtggtcg cgcctgggtc acggctatca ggtcgacgtg cgcgtggtgc
     9001 tgtgggagca agacgcggtg ctcgcgatac cgctgacggc gctgtttcgc gacgatcagc
     9061 ggtgggccgt gttcgtcgac gacgacggcc gcgccgccct gcgcgaggtc gtcgtcggcc
     9121 accgcaacgg gatggtggcc gagatcgtca gcggtcttgc ccggggagac cgggtggttc
     9181 tgcatccgag caaccgcgtg atcgacggcg tccggatcac cggcagaggc tgacgaggcc
     9241 ggggggccta cggcgccgca gtagaggcta gagccgcagg cccagccttg ctgccagctc
     9301 cccgaggaga gcatccaggc tcaaggattg cgaggtgtgt ccgaacacgt agcggtcggg
     9361 tcgcaccacg gcagcggccg agccttcgaa gagcctgtcg aagcgtcccc gggtggcttc
     9421 gagcccctcc agggtcgtgg tcgacatgcc gagggcgtcc acgatggccc gggatgcctc
     9481 gctcatggcc agatcggcct cggacctggc aaccaacgcg aagccggcac ccagccgatc
     9541 atccaggaga tactcctccc catcggcatc ccgcaccgca ggctggcgga aaagatagcc
     9601 cgtggagcca tgctcgctca cctgatccac catcaccacg ccgtcgcgca ggggcggtgc
     9661 ttcccgaccc tggccgtagc cggacgcgcg gttgccgtcc ggcacttcag gcggcttccg
     9721 ccccaccctt tgtgccgctt ccacggcggc cagatgctcc atgagcttgc cgatggccac
     9781 cgcccattcc accaggtcgg cggcatgagc cccccgttcc gcctgatagg aatccaggag
     9841 accttcgtct gccgcaccgc tcaagaccaa cgggagcttc caggccagat tgatcacgtc
     9901 gcgcattccg gcgttcagcc cctgacccag aaagggcggg gtctggtggg cggcgtcccc
     9961 cgcgagcagc acgcgaccct ttcgccaggt ctcagcggtg gcggcgtgaa actgatacac
    10021 ggcgctgcgc cgtatttcgt aggtgccccg tggtgtccac ggatcgatca gctcgcggat
    10081 cctctcgggc tctagcatct cctcccgggt ctcgccggga ttgagcttga actcccagcg
    10141 ccggaaaggg tccttggtcg cgatgtaggt ggacagcctg tcggggtcgc agacctgcag
    10201 cgtatccaaa cccagggtgt gcccgtcttt cagcacgacg tccaccacca gccagtcgtg
    10261 atcgtagccc aggtcgcgcc agccgatctc cagggcccgg cgcgcgccgc tcgccgcgcc
    10321 gtcgcaggcg accaggaacc gggcgctcgt ctcggaaagc gcgccgtccg cctgccgcgc
    10381 gcgaatggcg acttgcccac cggtttcttc gaaggtctcg acctggacct caaggcaccc
    10441 agtcaccccg gagtggtcca tcgccaaccg gcggagatgc gcctccagct ccggctgatc
    10501 gaacatggag atgggctgcc agccgttgac cccgaacgtg gcgtaaggct ggccaaagag
    10561 ccactcgcgt cgggagttcg cgaacccggc ccgctcgccg ggccgaaccg gctgcagcag
    10621 agcggcgagc tcgccggccc gccccacccg ctgaaagccg cgaaccacct cgccgtccag
    10681 ggcgacggcc cgcggcagcg ggtagacgtg cgcgtcgcgt tcgaaggcga tgactttcag
    10741 gcccgcgtcc gcgaagagga tggcggcggc agcgcccacc ggccccatgc ccacaatggc
    10801 gacgtcgtag ctgctcaatg tgcgtgctcc ccgctacccg gaatgagcag atccgcaatg
    10861 acttcggcgt gtctgcgcac cacatcgtcg tcgtacggat cgatgcctgc aaggcgctcg
    10921 gcctcgggag ccatggagaa gagaagggat cccgcccccg tgagtatgta atagaagtga
    10981 acgaagggga cgttgcgcac ggcccctgcg gatcgggctt cctcgaacgc ggcgcccaaa
    11041 cgctcgtacc agggcccgac gatgtgctct gcgagccact gtagccgcca gtcgtcgtcc
    11101 atgccctcgc ggatcatgag ccggttggcc tcggggaagc tcgccgagaa gcgcacgaag
    11161 ccggtaatga gcgcgcgaag ctgtgctgcc gggtccgagc caccttgccc ggagacgcga
    11221 acccgcaaca tgtgcggggc ctggccgaac caccaggtca ccgcctgctt ccacagcgcc
    11281 tccttcgtcc cgaagtggta ggcgatcaga ccacgcttga ccccggcaga ggcctcgatc
    11341 tgtcgcgtgg aagacccctc gtaacccgcc gcggcaaaac agcgcacggc ggcgtcgagc
    11401 aaccgatccc gggtgacctg ggcccgttcc tgaaccggcc cgcgatggct ttgattcggc
    11461 atggacggca aaaccttgtc atttgacgag ttattctata aataactcgt cattcgtcaa
    11521 gttttcatcg aggaggtcgc cgtgtccggc atgaccgaca aactctacgc gcgctttcgg
    11581 gtccccgacc tgaaccggca ggcgcgctct cgatcttccc ggcggcggct gggcagccag
    11641 gtgttcgact actggaaaga tcccaacggc tttaccatgg agcactttac ggacggcgat
    11701 ctgttcaacg catcctccgg atccgaaaaa tggccggtgg acgtggcttt ggggtctcac
    11761 tgaggaccgg aaggagcgcc ttagggaaac gtcatgacca gcgcgcgcga gatatcgacc
    11821 caacgggctg cagagacctg aagtgctgcc gggcggtctg atcttcggta ccttcctggt
    11881 gctcgtcctg gtgctcgccc tggtgctcgt cctggtgctc gtcgccggct ggtacgtgtg
    11941 ggagcgccgg cagcggcgaa cgcgcgcgat gcccggcggc ctgcacgagg agatcaccct
    12001 cccccaccgg caagcctggg agctgtacca caacaacctg tcgctctgct cgaagaagac
    12061 gcgggcgtgt ttgagcgagt tgtccatcga ctatgaaagc caccacatcg acctcatcga
    12121 gaccggccgt tacgagaacg tctcccggga gttcctgaag gtgaatcctg ccgggcttgt
    12181 tcccgtgctc gtgcatgagg ggcacccggt gtacgaatct cacgcgcaga tcgccttcgc
    12241 cgcggcccgc agcgacgacc cccaccgcct ggttcccgcc gaccctgacc agcgccgtca
    12301 gatggacgat tgggtgcgca agacgtcgct catcggcgac gaccccgtcg gcgcggttca
    12361 ccagaccgcc ggcaatgccg tccccggtct gacgctcccc ctgttcgcga ccatgctcga
    12421 acggatcccg ctgcggcggg ttctcgaggg cctgctcttt caccgactga agcggcgcgc
    12481 cttcatcctc atgatcatca agctatacgg catcgacagc ctgaccagcg caaagccact
    12541 gctccgcgtg gtccaggcca gccgcgacgc catgcaccgc cacctcgatg acctggaagc
    12601 gaccctgaag gacagcggcg ggccctggat cgtcggcgag cacttcagcc tcgccgacat
    12661 cggcatgatg gccatcctgg agcgcctgcg cgaggctgac tggctcgacg tgctggtgtc
    12721 aggccggcgc ccccgggtgg ccgcctactg gcgcgcgctg cagacgcgcc cgagctatca
    12781 gtcggcaatc gtcgcctgcc ggggcgaggc gatcgaacgc ggtaccgcca ggctcgtggc
    12841 cgcaaagcag aacaaccccg tgctgcgctt cgccttgatc gagacgatca cctgaacgcg
    12901 cggccatcca tcagacacga caatcacgaa agcgaggaca ccatgaagct agttagtttt
    12961 tccacgacag agaatccgaa accccgactc ggcgccgtgc gggacggcgg gatagcggat
    13021 ctgagcggtt cggacgggat cccggagacg atgctgggtc ttctgcatgc cggggacgct
    13081 gccatgaagg ccgcacacga ggccgctgaa gcggccgctg acaccgtgcc gctcgatcag
    13141 gtgacgctcc acccgccggt gctgacaccg ggaaagatac tcgccatcgg gctcaactac
    13201 ggcgaccaca tcgaggagag cggcatgggc aaacccaagc atcaggtgtg gttcaacaag
    13261 cagcacaact gcgtcaacgg accctatggc gacatcgcgc tgccgtcggt ctccgagatg
    13321 ctcgactacg aggccgagct ggtggtggtg atcggcaagc gctgcaagca cgtgccgcga
    13381 gagcgcgcgg cggaagtcat cgccggctac tgctgcggca acgacgtgag cgttcgcgac
    13441 tggcagatgc gcgcccagac catgcagatc ggtaagtcat tcgacaccca cggccccacc
    13501 gggccatggc tggtcacgcc ggatgaggtg ggcgaccccc acgacctgga catccgcgcc
    13561 gaggtcaacg gggaactccg ccagaactcg aatacgaagc acctgatcta cgactgttac
    13621 gacgccgtag cgcatctgac ccaggcgttc accctggacc cgggcgacct gctgttcatg
    13681 ggcacgccgg ccggtgtcgg ggcggcgatg aagcccccgc agtttctcaa ggccggagac
    13741 gtggtcaggg tggaaatcga gaagctcggc tacctcgaaa accgggtggt ccccgaagca
    13801 gccgagtgcg tgatcgagtg acctagcggg gatcgagccc gcgcttggcg cgctcggcgt
    13861 tcatgcgctc cttgtcgacg ccgagccgcg ggccgaggtc ttcctctttg accgtcccgg
    13921 ccatgtgccc gccggccagc ttgccgttgt acatgatctt cttcaccggc gtgacggcaa
    13981 gcacggtccg caacggcgag tcgagcaggt tgtagaagaa ctgctcgccc tccgggctgt
    14041 tgggattgag cttctgcgac agcgcggtgt agaaccactt cttggtttcc tcgtcgtcga
    14101 agaactctcc ggtgcccttg aaggtgatgg caccgcccgg cagctcctct gaagcgcccg
    14161 gcgggtagcc gcagctgctg acgttgacgg aaacgcggtt atcgcggcgg atggcaaccg
    14221 ttctatggcg atgcgcggca aaggtcagcc agatcttgcc gtcgtgccac acgaacgcgt
    14281 gggtgacccc taccggccag ccgtccttgg ttgcccacat gagcacgcat tcgacggagt
    14341 tgttcatcag ctggtcgact tcctcgtccg taaacgggta aatcgagacg atctcgtgat
    14401 cgtgagtttg cgcttgagcc ataagttccc cctggagtaa ctgccgtccc cgatcccggg
    14461 gaaaaagagc ggctagaata atgcaatcgc tcgatcggcg ataccgacga gccccaccgg
    14521 cctgctcgcg ccgatcggaa acagcaacgt cattccgctt aaacttcaac cggtaaagga
    14581 gcatccacgg taacgaccat gagcaacctg cgccgatcga cccaactcta cgaacgcgcc
    14641 aaggccgtca ttcccggggg catccccggc atccgggcgc cggaaaattt cgtcccgggt
    14701 gcttacccca tacttctggc cggaggccgc ggcggtcacg tcacggacgt ggacggcaac
    14761 gactatgtgg acttgctgct ggggtacgga cccgtgatcc tcgggcacgc cgagcaggcc
    14821 gtggacagcg cggcccacga acgcgccgcc cacggcttct gcctggatct ccccgaaccc
    14881 gtcatgatcg agctggccga gcagctcgta tccatggtgc cctctgcaga gcaggcgctg
    14941 ctgctgaaga cgggctccga tgccacgagc gctgcgatcc gcatcgccag gacccacacg
    15001 ggcaggtccg tggtcctgcg ctgcgggtac cacggctggc acgactggtg tcttcaggga
    15061 gatcccggcg tgccgtccgg caccaccgac accgtcatcg cgttccccta caacgacctc
    15121 gaccgcctgg ccgctctgct gacggcccac tcgggcgacg tggcgaccat catgctcacc
    15181 ccggtgggtc acgagttcga ccgccagatc gagcaacccg aaccaggttt tctggagggg
    15241 gtgcgcagcc tggccgacca gcacggcgtg gtgctcgcct tcgacgaggt tcgcacgggc
    15301 ttccgcgtcc acccgggcgg cgcccagggc ctctacggcg tcatccccga cctgaccgct
    15361 ctgggcaagg ccatgtcgaa cggttacgcg gtgacggccc tggtcggccg gcgggacgtc
    15421 atgcagagcg cccggagcac gttcatctca tccacctact tccccaacgg catggagatg
    15481 gcggcggctc tcgagaccct gaagctgatc gccgagcacg acgtgcttac cggcatcgcc
    15541 gctaccggcg ctgcgctcgc cggagggctg tcggaaatcg tcgcccggca cggcctgccc
    15601 gtctccctgt caccctacga cgagatgccc ttcctccatt tcgacccgga cctggcatca
    15661 ggccagcccg agcgacgaga cgctttctac ggcgccctgg cccggagcgg cgtattcgcc
    15721 cacccgcgcc atcacggatt tctctgctgg cgccacagcg ccgacgacgt gacgcgcgtg
    15781 ctggaggcgg tggatgccgc cgcggcggcg ctgggaagct agtccggccc cccgggcggg
    15841 cgggcgatgc ccgaccagtt tcccgccagc cggtcgtccg ccgccaggcg gacgtccacg
    15901 ggcaggccgg tgagatggga cattccggaa aacgggtcca cgtgggcagc acccgagctt
    15961 gcgagtatgt tcacgttcac gccgaggctg ccggccgcaa cgcgctggcc gctggcctga
    16021 tggccccagc cgtgggggac cgccaccacg ccggccagca gatcgtccga cgtccggacc
    16081 ggcaggcgga tggcgcccgc ggagctgcgc acgtcggcca ggtcgtcggg cttcaagccc
    16141 agggcggcgg cgtccgtggg gttcatgaag acgtagttgc ggccctggtc gccgcgcaga
    16201 aacccctcgt agttgtgagt ccaggaattg tgcgtggtga cctcccgttt ggttatcagc
    16261 cggaagggaa actcgctggc agacccttgc acgcgagccg aggcgaggg
//
LOCUS       DBNHQR010000223         7620 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1541496, whole genome shotgun sequence.
ACCESSION   DBNHQR010000223 DBNHQR010000000
VERSION     DBNHQR010000223.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7620)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7620)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7620
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1541496"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1359
                     /locus_tag="AC16VR_05190"
     CDS             <1..1359
                     /locus_tag="AC16VR_05190"
                     /inference="COORDINATES: protein motif:HMM:NF000658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HIR6010584.1"
                     /translation="RVLPGMDPHASCYGGHQFGNWAGQLGDGRAINLGEVIGTGGEHW
                     MLQLKGAGPTPYSRTADGLAVLRSSVREFVCSEAMYHLGVPTTRALSLVLTGEQVMRD
                     MLYDGRPAPEPGAVVCRVAQSFIRFGHFEILAARREPDLLRQLADFTIRTHFPHLAPG
                     SAPLARDDYLAWFAEVRDATVEMVVEWMRVGFVHGVMNTDNMSILGQTIDYGPYGWLE
                     NYDPNWTPNTTDAAGRRYRFSYQPGIAQWNLTRLAEAIYPLVEAAEPLEELLAGFEKR
                     YVESWQSMMARKLGLRSPQPADVVLLNELDEVLQLAETDMTIFFRRLADLSPADPASD
                     PVTVVREAYYLPDQLAGETLERIGNWLEDYVARLRQEGVGNEERRQAMNAVNPKYVLR
                     NYLAQLAIDDAESGDFALVGELLDLFRRPYDEQPARERFAARRPDWARTRVGCSMLSC
                     SS"
     gene            1450..2958
                     /locus_tag="AC16VR_05195"
     CDS             1450..2958
                     /locus_tag="AC16VR_05195"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013784.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0097054 - L-glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HIR6010585.1"
                     /translation="METPLFLELLNLLASIFVFLLGLGLIAVIVIYIVDRTQTKHAVR
                     RNFPVIGRFRYVFEHLGEFFRQYFFALDREEMPFNRAERTWVYRAAKGEDTTVAFGST
                     RDLRPPGTVLFVNCAYPTLDEDAAPLGRVTIGPDCEHPYDCGSLFNISGMSFGAVSRP
                     AVTALSNGARMAGVWMNTGEGGLSSYHLDGGADIVFQIGTAKYGVRNERGELCDEKLA
                     EMAAHRQVKMFEIKLSQGAKPGKGGILPGNKVTDEIARIRLIPVGSDSISPNRHPEIE
                     NEGDLLDMIDRVRRATGKPVGFKTVIGAYGWLEDLFAEINARGPASAPDFITIDSADG
                     GTGAAPMSLIDYMGLPIRESLPLVVDLIEAYGLRERIRVVASGKMVTPGEAAWAVCAG
                     ADFVVSARGFMFALGCIQALQCNKNTCPTGVTTHDPDLQRGLDPVDKAERVRRYAQHM
                     HRQLGMIAHSCGVAEPRQLKRFHCRIVTQTGISVPLDELYPPLVRDPARLSA"
     gene            3092..4351
                     /locus_tag="AC16VR_05200"
     CDS             3092..4351
                     /locus_tag="AC16VR_05200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450 family protein"
                     /protein_id="HIR6010586.1"
                     /translation="MTAIDRTVFECLRFTTAVADRLVTGSRMSGPAFLENPYGEYARL
                     RARGEVIRNFSIGGWNVLGFEAVRDAMRDARFGVDVRKSPFVDRIVRLVSRGRPFRML
                     DEPTMLNIDPPDHTRLRRLARQGFLHRYVCALEPKIEGLVEECLATVQGGMFDLMDTL
                     ARPLPANVIADILGVPREDRARFQAWSELIAKNGRNIEYDALQQTDAVFNELIDYLAQ
                     ITDAKRADPGDDLISHLIAAEEDGDRLSADEIHATSALLLIAGHETTTRLIGNAMWLL
                     LSHPEQLEILRANPALMPNAVEEALRFEAPVQHMTRIALEDMDFHGVHIRKHQLCEVY
                     IGSANRDPQANPNPDVFDICRENIQHVGFGYGIHLCLGAQLARLETQMALKMLLERFP
                     RLALTGAPPAWGDSTFVRGLDELLLAA"
     gene            complement(4353..6011)
                     /gene="oxc"
                     /locus_tag="AC16VR_05205"
     CDS             complement(4353..6011)
                     /gene="oxc"
                     /locus_tag="AC16VR_05205"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF006721.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HIR6010587.1"
                     /translation="MMATLDGTTLIARSLKQQGVEYMFGVVGFPVFGIAAAAQAEGIT
                     YIGMRNEQSASYAAQAAGYLTGRPQACLVVSGPGVIHAFAGLANAQQNCWPMILIGGA
                     SPTYQNGMGAFQEERQVDLAKPYCKYAHGIDSVARIPYYVEQAIRTSLYGRPGPVYLD
                     LPDDIIRGTVEEDEVVSAAPVGPAPRSQARPEDVEAALCVLESAERPLVVVGKGMAWA
                     RAENEVREFIERTQLPFLASPMGKGVLPDDDPLSVGAARSTALKEADVVFLMGARLNW
                     IMHFGLPPRFAPDVRIIQLDIAAEEIGTNVPAEVALLGDGQAVAAQLNAALDNRQWFY
                     PADAPWRTLLREKASDNQAAVAPMIDAEAMPMNYYRAYRDIEAWLPEDAIIIGEGANT
                     MDIGRTQMANRSARNRLDAGTYGTMGIGLGFTIAAAVVHPDRPVVAVQGDSAFGFSGM
                     ELETACRYKLPIKVIVLNNGGIGAGIDELPEDGPIPPFSLTPGARYEGIMETLGGRGL
                     YVEDPKDLRGALDEAMAFDGPALVNVRIDPRAGRKQQQFGWLTT"
     gene            complement(6117..>7620)
                     /locus_tag="AC16VR_05210"
     CDS             complement(6117..>7620)
                     /locus_tag="AC16VR_05210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR6010588.1"
                     /translation="LENRGGVGGAAVTEEFHPGFRNSVASYTVSLLNPKVIQDLRLAE
                     HGLRIVNRPYSNFMPLPDGDYLRTGATIEETQQQFARFSRTDAERLPGYYAALEAGAD
                     LLRGMLLETPPNAGGGLRDVMRAVRLGGRLRRLSTAARRDVLDLFTKSAGELLEYWFE
                     SDPVKALFGFDAVVGNYASPYAPGSAYVLLHHVFGEANGQAGQWGHAIGGMGAITQAM
                     LKEALARGVTVRTDADVRLVRVRNGRANGIVLEDGEEIGARAVASNLNPKLLFTRLIE
                     AEALDDDFRARIARYRCGSGSFRINVALSQLPRFECAPPGQPELGTRSGIIMAPSLKY
                     MERAYFDAREHGWSRKPIVEMLIPSTVDDSLAPPGQHVASLFCQQFAPTLDGQSWAEA
                     RDAAAQTVFDTVEGYAPGFRQSILGAMVLSPEDLETEFGLTGGDIFHGALSLDQLFSA
                     RPVLGHGDYRAPVRGLYMCGSGTHPGGGVSGAPGHNAAREIIRDFRARRL"
BASE COUNT         1253 a         2490 c         2540 g         1337 t
ORIGIN      
        1 cgtgtgctgc ccggtatgga tcctcacgcg tcctgctacg gcggccatca gttcggcaac
       61 tgggcgggtc agctcggcga cggccgggca atcaacctcg gcgaggtgat cggcaccgga
      121 ggcgagcact ggatgctcca gctcaagggc gccggcccca cgccctactc gcggacggcc
      181 gacggcctgg cggtgcttcg ctcctccgtg cgcgagtttg tctgctccga agccatgtat
      241 cacctgggcg tccccaccac ccgggctctg agcctcgtgc tgacgggcga gcaggtcatg
      301 cgggacatgc tgtatgacgg gcggccggca ccggagccgg gcgccgtggt ctgccgggtg
      361 gcgcagtcgt tcatccggtt cggccacttc gagattctcg cggcacggcg ggagccggat
      421 ctgctcaggc agctcgccga cttcacgata cgaacacact tcccccatct ggcaccaggc
      481 tctgcgcccc ttgcaaggga cgactatctg gcctggttcg cggaagtgcg tgacgccacc
      541 gtcgagatgg tcgtggaatg gatgcgtgtc gggttcgtgc acggcgtgat gaacacggac
      601 aacatgtcca ttctgggcca gaccatcgat tacggcccct acggctggct cgagaactac
      661 gacccgaact ggacgcccaa cacgaccgat gctgcaggcc ggcggtaccg gttttcgtac
      721 cagccgggaa tagctcaatg gaacctcacc cgactggcgg aggccatcta tcctctcgtt
      781 gaagccgcag agccgctgga ggaactgctg gccggattcg agaagcgcta cgtggagtca
      841 tggcagagca tgatggctcg gaagctcggt ctgagatcgc cccagccggc cgacgtcgtg
      901 cttttgaacg agctcgacga ggtgctgcag ctggccgaga ccgatatgac catctttttc
      961 cgccgcctgg ccgatctgag tccggccgat ccggcaagcg atccggtgac ggtggtccgc
     1021 gaggcttact atctgcccga tcagctcgcc ggcgagaccc tcgagcgcat cggcaactgg
     1081 ctcgaggact acgtagcccg gctgcggcag gaaggtgtgg ggaacgagga gcggcgccag
     1141 gcaatgaacg ccgtgaatcc gaagtacgtc ctgcgtaact acctggccca gctcgccatc
     1201 gacgatgccg agtccggcga cttcgctctg gttggcgagc tgctcgatct cttcaggcgc
     1261 ccctacgacg agcagccggc acgtgagcgg tttgcggccc ggcggccgga ctgggcaaga
     1321 acgcgtgtgg gctgctccat gttgtcctgc agctcctgat atcccgcccc cagtccgggc
     1381 ggcgtccgac gggtcgattg tccgctttcg gtgctctatt ctcggtccgt ggctatcggg
     1441 aggctgccca tggaaacgcc cttattcctc gagcttttga acctgctcgc cagcatcttc
     1501 gtctttctgc ttggcctcgg gctgatcgcg gtgatcgtga tctacatcgt cgatcggacc
     1561 cagacgaaac atgcggttcg ccgcaatttt cccgtcatcg ggcgctttcg ttacgtgttc
     1621 gagcacctcg gagagttctt ccgacagtac ttttttgcgc tggatcgcga ggagatgccg
     1681 ttcaaccgag cggagagaac gtgggtgtat cgcgccgcaa agggcgagga caccacggtg
     1741 gccttcggct ccacgcgcga tctgcgcccg ccgggcacgg tgctgttcgt caactgcgcc
     1801 taccccaccc tcgacgagga cgcggctccc ttaggccgcg tcaccatcgg gcctgattgc
     1861 gagcacccct acgactgcgg ttctcttttc aacatctccg gcatgagctt cggcgccgtc
     1921 tcgcgaccgg ccgtcaccgc cctttccaac ggcgcgcgca tggcgggcgt gtggatgaac
     1981 accggtgaag gcgggctgtc ctcataccac ctcgacggag gggcggacat cgtctttcag
     2041 atcgggaccg ccaagtacgg cgtgcgcaac gagcgcggcg agctttgcga cgaaaagctc
     2101 gccgagatgg ccgcgcaccg gcaggtgaag atgttcgaga tcaagctctc ccagggcgcc
     2161 aaaccgggga aaggcggcat cctgccgggt aacaaagtta ccgacgagat cgcgcggatt
     2221 cgtctgatcc cggtcggttc ggactccatc agccccaatc gtcaccctga gatcgagaac
     2281 gagggagacc tgctggacat gatcgatcgc gtcaggcggg cgacggggaa gccggtgggc
     2341 ttcaagaccg tgatcggggc gtacggctgg ctcgaggacc tctttgcaga aatcaatgct
     2401 cgggggccgg cctcggcacc ggacttcatc accatcgaca gcgccgacgg cgggaccggg
     2461 gctgctccca tgagcctgat cgactacatg ggtcttccca taagagaaag tcttccactc
     2521 gtggtcgacc tcatcgaggc gtacggtctg cgcgagcgca tcagggtcgt cgccagcgga
     2581 aagatggtca caccgggaga ggcggcttgg gccgtgtgcg cgggtgccga cttcgtagtc
     2641 tccgcccggg gcttcatgtt tgccctgggc tgcatacagg cgctgcagtg caacaagaat
     2701 acctgcccca ccggcgtgac cacgcacgac cccgacctgc agcggggtct ggaccctgta
     2761 gacaaggcgg aacgcgttcg tcgttacgcg cagcacatgc accgccagct cggcatgatc
     2821 gcccactcct gcggtgtggc cgagccccgc cagctcaaac gctttcattg ccgcatcgtc
     2881 acgcagacgg gaatttccgt gcctttagac gagctgtatc cgccgctcgt cagggatccc
     2941 gcccggctgt ccgcatagtc ggagctggtc cgcgctgggc ctccaatctg tgaggcggcg
     3001 cacaggttcg cgttcgaagc cttgtcgggg catcggtggg tgcaacaatg aggtgtcggc
     3061 gcaggccagc gcttctaacg ggagtcccgc catgacggcc atcgacagga cggtgttcga
     3121 gtgcctacgc ttcaccacgg ccgtggccga tcgcctggtg accggcagcc ggatgagcgg
     3181 ccccgctttc ctggagaacc cctatggcga atatgcaagg ctccgcgccc ggggtgaggt
     3241 catacgaaac ttcagcatcg gcggatggaa cgtcctgggc ttcgaagcgg tccgagacgc
     3301 catgcgggat gcgcgtttcg gcgtcgatgt tcgcaagagc cctttcgtgg acaggatcgt
     3361 gcggctcgtg agccgaggca ggccgtttcg aatgctcgat gaaccgacga tgctcaacat
     3421 agacccgccg gaccacaccc ggctgcgtcg gctggcccgt cagggctttc tccaccggta
     3481 cgtctgcgcg ctggagccga agatcgaggg cctggtcgag gagtgcctgg cgacggtcca
     3541 gggcggtatg ttcgatctca tggataccct ggcgcgtccc ctgccggcta acgtgattgc
     3601 cgacatcctg ggcgtgcctc gggaagaccg cgcaaggttt caggcctggt cggagctgat
     3661 cgcgaagaac ggccgcaaca tagagtacga tgccctgcaa cagaccgacg ccgttttcaa
     3721 cgagctgatc gactatcttg cgcagatcac cgacgccaag cgggccgatc cgggcgacga
     3781 tctgatcagc catctgattg ccgccgaaga ggatggcgac cggctgtccg cggatgagat
     3841 acatgccacc agcgccctgc tgctgattgc cggtcacgaa accaccaccc gcctgatcgg
     3901 caacgccatg tggctgctgc tcagccatcc ggagcagctc gagatcctgc gcgcgaatcc
     3961 ggccctcatg ccaaacgccg tcgaggaagc gcttcggttc gaagcgcccg tgcagcacat
     4021 gacgcggatc gctctggagg acatggactt tcacggcgtg catatccgca agcaccagct
     4081 gtgcgaggtg tacatcggct cagcgaatcg cgatccccag gcgaatccga acccggacgt
     4141 cttcgacatc tgccgtgaga acattcagca cgtgggcttc ggctacggca tccacctttg
     4201 cctgggggcc cagcttgcgc ggctcgagac gcagatggcg ctgaagatgc tgctggagcg
     4261 ttttccccgg ctcgccctga cgggcgcgcc ccccgcctgg ggtgacagca ccttcgtccg
     4321 cggcctcgac gagcttctgc tggccgcctg attcaggtcg tcagccagcc gaactgctgc
     4381 tgcttgcggc cggcccgcgg atcgatgcgt acattcacga gggcggggcc atcgaaggcc
     4441 atggcctcgt ccagcgcgcc ccggaggtct ttgggatcct ccacgtaaag gccgcgcccg
     4501 ccgagcgtct ccatgatgcc ctcatagcgc gcgcccgggg tcagcgagaa cggcgggatg
     4561 ggcccgtctt cgggcagctc gtcgatgccg gcgccaatgc cgccgttgtt gaggacgatg
     4621 accttgatgg gcagcttgta gcggcaggcg gtctcgagct ccatgccgga gaaaccgaaa
     4681 gcgctgtcgc cctgcacggc caccacgggc cggtccggat gaacgacggc ggccgcgatg
     4741 gtgaagccca ggccgatgcc catggttccg tacgtgcccg cgtccagacg gttgcgggcc
     4801 gaccggttcg ccatctgggt gcggccgatg tccatggtgt tggccccttc cccgatgatg
     4861 atcgcgtctt ccggaagcca cgcctcgatg tcgcggtagg cccggtagta gttcatcggc
     4921 atggcctcgg cgtcgatcat gggcgccacg gctgcctggt tgtccgaagc cttctcccgc
     4981 agcaatgtcc gccacggggc atcggcggga tagaaccact ggcggttgtc caaggcggcg
     5041 ttgagctggg cggccacggc ctggccgtcg cccagcagcg ccacctccgc ggggacgttg
     5101 gtgccgatct cctcagcggc gatgtccagc tgaatgatcc gcacgtcggg agcgaaccgc
     5161 ggtggcaggc cgaagtgcat gatccagttc aggcgcgcgc ccatcaggaa gaccacgtcg
     5221 gcctccttga gcgccgtgct gcgcgcggct cccaccgaaa ggggatcatc gtccgggagc
     5281 acgcccttgc ccatggggga ggcgagaaac ggcagctggg tccgctcgat gaactcgcgc
     5341 acctcgttct cggcgcgcgc ccatgccatc cctttgccga ccacgacgag tggccgctcg
     5401 gccgattcga gcacacagag ggctgcctcc acgtcctcgg gtctggcctg ggagcggggc
     5461 gccgggccca cgggcgcggc gctgacgact tcgtcctcct ccaccgtgcc gcgaatgatg
     5521 tcgtcgggca ggtcgaggta gacgggcccg ggccggccgt agagcgacgt tcgaatggcc
     5581 tgctccacgt agtagggaat ccgcgcgacg ctgtcgatgc cgtgggcata tttgcagtag
     5641 ggcttggcca gatccacctg gcgctcttcc tggaacgcgc ccatgccgtt ctggtaggtg
     5701 ggggaggcgc cgccgatgag gatcatgggc cagcagttct gctgggcgtt cgccagcccg
     5761 gcgaaggcgt ggatcacgcc gggacccgaa accaccagac atgcctgggg acggccggtg
     5821 aggtagccgg ccgcctgagc ggcgtagctc gcggactgct cgttacgcat gcctatgtag
     5881 gtgatgccct ccgcctgagc ggcggcggcg atgccgaaca ccggaaagcc cacgacgccg
     5941 aacatgtact cgacgccctg ctgcttgagg ctccttgcga tgagcgtagt gccgtctaag
     6001 gttgccatga ttccctccct cgctttgtta tctgcttctc tgcggtggct ctggcgtgcc
     6061 ccgaggggcc gagtatagca aggccgccgg cccctctcgc ttcgaggtgt gtccgttcag
     6121 agccggcgcg cccggaagtc gcggatgatc tcccgggcgg cgttgtggcc cggtgccccg
     6181 ctcaccccgc ccccgggatg ggtgccggag ccgcacatgt acaggccccg cacgggggcg
     6241 cgatagtcgc cgtggccgag caccggccgg gccgagaaga gctggtccag ggacagggcg
     6301 ccgtgaaaga tatccccgcc cgtgagcccg aactcggtct cgaggtcctc cggggagagc
     6361 accatggcgc cgagaatgct ctgcctgaac ccgggcgcgt agccctccac cgtgtcgaag
     6421 acggtttgcg cagccgcatc gcgggcctcg gcccagcttt gcccgtccag cgtgggggcg
     6481 aactgttggc agaagaggct ggcaacgtgc tgccccggcg gagcaagcga gtcgtccacc
     6541 gtgctgggaa tgagcatctc gacgatgggc ttccgggacc agccatgctc gcgggcgtca
     6601 aaataggcgc gttccatgta cttgaggctc ggcgccatga tgatgcccga ccgggtgccg
     6661 agttccggct ggcccggtgg agcgcactcg aagcgcggaa gctggctcaa ggccacgttg
     6721 attcggaagg aacccgatcc gcatcgatag cgggcaatac gcgccctgaa gtcgtcgtcc
     6781 agcgcctcgg cttcgatgag ccgggtgaac agcagctttg ggttgaggtt cgacgctacc
     6841 gcccgggcgc cgatctcttc cccgtcttcg agcacgatac cgtttgcacg accgttgcgg
     6901 acgcgaacga gccgcacgtc ggcgtccgtt ctcaccgtta ctccccgagc gagcgcctct
     6961 ttgagcatcg cctgggtgat ggcgcccatg cccccgatcg cgtgtcccca ctgacccgcc
     7021 tggccgttgg cttcgccgaa aacgtggtgc agcagaacgt aggccgatcc cggggcgtag
     7081 gggctcgcgt agttgccgac gacggcgtcg aagccgaaca gcgccttgac cggatcggac
     7141 tcgaaccagt actcgagcag ctcgccggcg cttttcgtga acagatcgag cacatcgcgt
     7201 cgcgctgcgg tggagaggcg gcgcaggcgg ccgccgagac ggacagcgcg catcacgtcg
     7261 cgaagcccgc cgccggcgtt gggcggggtt tccagaagca tgcctcgcag cagatcggcg
     7321 ccggcctcga gcgcggcgta gtacccggga aggcgttccg cgtctgtccg ggagaaccgg
     7381 gcgaactgct gctgagtttc ctcgatggtg gcccccgtgc gcaggtagtc tccgtcgggg
     7441 agcggcatga agttcgagta tggccggttg acgatgcgca ggccgtgctc ggcgagcctg
     7501 agatcctgga tgaccttcgg gttgagcagg ctgaccgtgt agctggccac ggaattgcgg
     7561 aagcctggat ggaactcctc cgtgacggca gccccgccca cccccccccg gttctccagg
//
LOCUS       DBNHQR010000224         4379 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1731661, whole genome shotgun sequence.
ACCESSION   DBNHQR010000224 DBNHQR010000000
VERSION     DBNHQR010000224.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4379)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4379
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1731661"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..717)
                     /locus_tag="AC16VR_05215"
     CDS             complement(43..717)
                     /locus_tag="AC16VR_05215"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008173895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HIR6010589.1"
                     /translation="MAGGSTERGDERDALVALIESEVADTADYTGRSKLRPAVLEAMR
                     AVPREEFVPPEEAYAAYANVPLPIGHFQTISQPYVVALMTDLLDPEPGDVMLEVGTGS
                     GYQAAVLSTLVSRVYSLEVVPQLAASAAERLARLGYDNVVTREGDGNLGWPDHAPYDG
                     IVVTAGAPEVPPALIDQLKPGAALVIPVDAGFNQMLRRITRDPDGDVHTEDVLPVAFV
                     PLVKAT"
     gene            909..1736
                     /locus_tag="AC16VR_05220"
     CDS             909..1736
                     /locus_tag="AC16VR_05220"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6010590.1"
                     /translation="MPKDNADQIEFWNGPTASNWVAAEERMDELLAPITTAILQAAAV
                     SPGEAVLDVGCGCGATSRAMAAVGARVTGVDVSAPMLARARQRADDESLSFIEADAQS
                     HPFDPGFDLLFSRFGVMFFADPVAAFANLRGALNPGGRLCFICWRAPGENPWLSIGMA
                     AAQPFLPPAETPDPRAPGPFAFADPDYLASVLADAGFEDVAITPHNPEMRLGADLTEA
                     MEFVTGVGPLSNVLKELAADLREAALAAVREALAPFAGPSGVLLGGACWIAQGRRGA"
     gene            complement(1762..2952)
                     /locus_tag="AC16VR_05225"
     CDS             complement(1762..2952)
                     /locus_tag="AC16VR_05225"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF006050.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR6010591.1"
                     /translation="MQTFVTHLECSLTGEHYPADQVHGLSAAGRPLLVRYDLTGLSRA
                     VGKADIAGRQGGFWRYREFLPVRRTENVVSLGEVVTPMIPIAGPAGGSVWVKDEGRLP
                     TGSFKARGLALAVAMARELGLSHLAMPTNGNAGAALAAYAARAGLRATVLCPSDTPEI
                     NVREIAMQGAEVWRVDGLINDCGRIVGEAAGRTGWFDLSTLKEPYRIEGKKTMGIELA
                     EQLGWRLPKAIFYPTGGGTGLIGMWKAFAELEAIGWIGPERPQMFAVQAEGCAPIVRA
                     FERGDEHAELWENAHTAAAGIRVPAAVGDFLILRAVRESGGAALAVSDADLLAAQRRI
                     AAQEGLLLCPEGAATYVAWRQALEAGLVGAEDEVVLFNCATGLKYPMPDESRTFTRLD
                     DLIG"
     gene            complement(3006..3308)
                     /locus_tag="AC16VR_05230"
     CDS             complement(3006..3308)
                     /locus_tag="AC16VR_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009614101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family probable chaperone protein"
                     /protein_id="HIR6010592.1"
                     /translation="MLALVFSAVAVAAMGLDAAKAQGLVGEQHDGYLGIVSSATPEVR
                     ALVSDVNAKRRAEYQRIAQTNGIDVKDVELLAGQKAIQRTAPGHYVKLEGQSWRPK"
     gene            complement(3354..3536)
                     /locus_tag="AC16VR_05235"
     CDS             complement(3354..3536)
                     /locus_tag="AC16VR_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HIR6010593.1"
                     /translation="MLLALALALAVLAAAGCTPTVKVATDEPITINLNVNIKHEILVR
                     VDKELNDLFGEDSDLF"
     gene            complement(3681..>4379)
                     /locus_tag="AC16VR_05240"
     CDS             complement(3681..>4379)
                     /locus_tag="AC16VR_05240"
                     /inference="COORDINATES: protein motif:HMM:NF023167.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane phospholipid transport protein
                     YdbH family protein"
                     /protein_id="HIR6010594.1"
                     /translation="GEVVAAGIAGEARVKLREDGWQATADTLSVAVADPGFPLRDLRF
                     AAELTPATALLSAVRGRALGGELFIDRLAYDMNDATAAFLVIATGIELADVLALEGES
                     VTGSGILDGELPVELLEGGTRITNGRFVARAPGGVLRYPDAGKAAEALGQQVGGFALA
                     PLVDFRFEVLDAHVDLQPSGDLLLGVRLEGRSPEFERPYHYNLNISENIPALLKSLRI
                     SEEFQRRLEEVLQR"
BASE COUNT          744 a         1498 c         1418 g          719 t
ORIGIN      
        1 catccatgac gtcgcaggaa gctaaggttg cgcggacccc tcctaggtcg ctttcacgag
       61 cggcacgaat gccaccggca ggacgtcctc cgtgtggacg tcgccgtcgg gatcgcgggt
      121 aatccgcctg agcatctggt tgaacccggc gtccaccgga atcacgaggg ccgcgccggg
      181 cttgagctga tcgatcaatg ccggcggaac ttccggagca ccggcggtca ccacgatgcc
      241 gtcgtagggc gcgtggtccg gccagcccag attgccatcg ccctcccggg tgaccacgtt
      301 gtcgtatccc agccgggcca gccgctcggc ggcgctggcg gcgagctggg gaaccacctc
      361 cagggagtag acgcggctga ccagggtgct cagcaccgcc gcctggtatc cgctccccgt
      421 gccgacttcc agcatcacgt cgccgggctc cggatccagc agatcggtca tgagggcgac
      481 gacgtatggc tgcgagatcg tctggaagtg tccgatgggc agcggcacgt tggcgtaggc
      541 ggcgtaggct tcctccggag gcacgaactc ttcccgcggc acggcgcgca tggcttcgag
      601 cacggcgggt cgcagcttcg agcggcccgt gtaatcggcg gtatccgcca cttcactctc
      661 gatcaacgca acgagcgcgt cgcgctcgtc cccacgttcg gtagatcccc ctgccatcgc
      721 ttctgatcac cccgccggca tcgcccttgc ctcgagttta cccgagcccg cgcaccgggc
      781 cattgaggga ctccgaaaaa aggcgggcat ccctgggcag ggtgaccggg cggcggcgtg
      841 cttctagaat ctctcttcgc cgtgcggttt agccccgcac ccaccggaaa cacactgacg
      901 aaatagacat gccaaaagac aacgccgatc agatcgaatt ctggaacggg cccacagcca
      961 gcaactgggt ggccgcagag gaacgcatgg acgagctgct ggcccccatt accaccgcca
     1021 tactccaggc cgcggccgtg agccccggcg aggccgtcct ggacgtcggg tgcggttgcg
     1081 gcgccacgtc gagggccatg gcagcggtcg gagctcgggt gaccggtgtg gacgtatccg
     1141 cgcccatgct cgcccgggca cgacaacgcg ccgacgacga gtccttgagc ttcatcgaag
     1201 cggatgccca gagccacccc ttcgatcccg gtttcgatct cctgttctcc cggttcgggg
     1261 tgatgttctt cgccgatccg gtagcggctt tcgccaacct gcggggcgcg ctgaaccccg
     1321 gggggcgcct gtgtttcatc tgctggcggg cccctgggga gaatccctgg ctgagcatcg
     1381 gcatggccgc cgcgcagccc ttcctgccgc cggcggagac gcccgacccc cgagcgccgg
     1441 ggccgtttgc cttcgccgat ccggattatc tggcttcggt gctggccgat gccgggttcg
     1501 aagacgtggc catcacaccc cacaatcccg agatgcgcct gggtgcagac ctgacagagg
     1561 ccatggagtt cgtgacaggg gtcggaccgt tgagcaacgt cctcaaggag ctggctgcgg
     1621 atctgcggga ggccgcgctg gccgccgtgc gggaggcttt ggcgcccttc gccggcccct
     1681 caggcgtcct gctgggaggc gcgtgctgga tcgcccaagg gcgacggggc gcctgaacgg
     1741 cgggcgggtc gcccgccggg atcagccgat gaggtcgtcg agcctggtga aggtccggct
     1801 ctcgtcgggc atgggatact tcagccccgt ggcgcagttg aagagaacga cctcgtcctc
     1861 ggcgccgacc aggcccgcct ccagggcctg gcgccaggcg acgtaggttg cagcgccctc
     1921 ggggcagagc agcagaccct cctgcgccgc aatgcgccgc tgggcagcga ggaggtctgc
     1981 atcgctcacc gccagggcag cgcccccgga ctcgcgcacg gcgcgcagaa tgagaaagtc
     2041 gcccacggcg gcgggcaccc ggataccggc tgccgccgtg tgcgcgttct cccacagctc
     2101 ggcatgctcg tcgccgcgct cgaacgcgcg aacgatgggg gcacagcctt cggcctgcac
     2161 ggcaaacatc tgcggtcgct ccggcccgat ccagccaata gcctcgagct cggcgaacgc
     2221 tttccacatg ccgatcagac cggttccacc gccggtcggg tagaagatgg ccttgggcag
     2281 gcgccagccg agctgctcgg cgagctctat gcccatcgtc ttcttgccct cgatccggta
     2341 gggctctttc agggtggaaa gatcgaacca accggtacgt ccggccgcct cgcccacgat
     2401 gcgcccgcag tcgttgatga gcccgtccac gcgccatacc tccgcgccct gcatcgcgat
     2461 ctcgcgcacg ttgatctccg gggtatccga gggacacagc acggtggccc ggaggccggc
     2521 cctcgccgca taggccgcaa gtgccgcccc ggcgttaccg ttggtgggca tggccaggtg
     2581 ggaaagaccc agctccctgg ccatggccac ggccagagcg aggcctcttg ccttgaacga
     2641 accggtgggc agtcggccct catccttgac ccagacgctg ccgccggcag ggccggcaat
     2701 cgggatcatg ggggtgacga cttctcccag ggagaccacg ttctccgtgc gacgcacggg
     2761 cagaaactcc cggtaacgcc agaagccgcc ctggcggccg gcgatgtccg ccttgcccac
     2821 cgctctcgaa aggcccgtca ggtcgtatct gacgagcagc ggtcgccccg cagcagagag
     2881 gccgtggacc tgatcggccg ggtagtgctc cccggtaagc gagcactcga gatgggtaac
     2941 gaaggtctgc aaagcggttt aggactgggg ttgggcgagc ccgctctcgc agccaggctc
     3001 gccggctatt tcggccgcca gctctgacct tcgagcttga cgtagtgacc gggggcggtg
     3061 cgctgaatgg ccttctggcc ggcaagcagc tccacgtcct tgacgtcgat gccgttggtt
     3121 tgagcgatgc gctgatactc cgcgcgccgc ttggcattga catcggagac gagggccctg
     3181 acttcaggcg tggcggacga gacgataccc aggtaaccgt cgtgctgctc tccgaccaga
     3241 ccctgtgctt ttgccgcatc caggcccatg gccgcaacgg ccactgccga gaagaccagc
     3301 gcgagcatga aaacgatttg ccgaaagatt ggtttcatat tcatcgcgtc gccttaaaag
     3361 agatcactgt cttcgccgaa caggtcattc agctctttgt ccactctcac gagaatctcg
     3421 tgcttgatgt tgacgttaag gttgatggtg atgggctcat ccgttgcgac cttcaccgtc
     3481 ggggtgcatc ccgcagcggc gagaacggca agagcaaggg cgagcgcgag cagcatccct
     3541 gaggcggccg tacgcggcct gaccggtggg atccggcggc aggaaaccgg caattcgaac
     3601 gagttactca tcatcaaaga gtacctggcg cactgtgaac cggagctgaa cattacccag
     3661 cgccgacacc ccccgcaagc tcacctttgc agaacctctt ccaggcgtcg ctggaactct
     3721 tcggaaatgc gcagggattt gagcagggcc ggaatgttct ccgagatgtt caggttgtag
     3781 tgatagggcc gctcgaattc cggactgcgc ccttccaacc gaaccccgag caggaggtcg
     3841 ccgctcggct ggagatcgac atgcgcatcg agcacctcga atcggaaatc caccagcggc
     3901 gcgagagcga acccgccgac ctgctggccg agcgcttcgg ccgccttccc cgcatccgga
     3961 tagcgcagaa cgcccccggg cgcccgagcg acgaaacgtc cgttcgtgat cctggtgccg
     4021 ccctcgagga gctcgacagg cagctcgcca tcgagaatgc ccgatccggt caccgactct
     4081 ccttccagag ccagcacgtc ggctagctcg atcccggttg cgatcaccag aaaagcggcg
     4141 gtggcgtcgt tcatgtcgta ggccagtcgg tcgatgaaga gctccccgcc aagcgctctg
     4201 ccccgaaccg cgctgagcag cgccgtagcc ggcgtcagct ccgcagcaaa ccgcagatca
     4261 cgcagaggaa agccggggtc tgcaaccgcc acgctcagcg tatcggccgt agcctgccag
     4321 ccgtcttcgc gcagcttcac ccgggcctca ccggcaatgc ctgccgccac cacttcgcc
//
LOCUS       DBNHQR010000225         3046 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_528954, whole genome shotgun sequence.
ACCESSION   DBNHQR010000225 DBNHQR010000000
VERSION     DBNHQR010000225.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_528954"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="AC16VR_05245"
     CDS             <1..279
                     /locus_tag="AC16VR_05245"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017335953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HIR6010595.1"
                     /translation="VGPLPADSVFNRAIDGEFDVVLAMYHDQGHIAIKVHNFHESTTA
                     TLGIPFVRTSVDHGTAFDIAGSGRADERGLAAALDAARALSQGQLARL"
     gene            351..1433
                     /locus_tag="AC16VR_05250"
     CDS             351..1433
                     /locus_tag="AC16VR_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007974217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HIR6010596.1"
                     /translation="MKAAVFREINQPMEIEEVALSKPGPREVLVRTAAAGVCHSDLHF
                     FNGTYPGLLPMVLGHESAGVVEAVGSDVHYVKPGDHVITCLSVFCGHCEFCLTGHLSL
                     CQEPETRRAPDQEPRISHDGAPVQQFANLGSFAEMMLVHEHAVAKIREDMPLDRAALI
                     GCGVTTGVGAVIHTAKVEPGSTVAVIGCGGVGLSCINGAAIAGASRIIAVDMVASKLD
                     LARKFGATDVVNAAEMDAINEVKALTGGGVHYSFEAIGLKATAEQAFGMLRNGGTATV
                     IGMIPPGDMVSLHGPDFLFEKKIQGSMMGSNRFRVDMPRFVDLYLQGKLHLDDLVSSR
                     IRLEDVNDAMAQLETGEIARNVIVFD"
     gene            1442..2287
                     /locus_tag="AC16VR_05255"
     CDS             1442..2287
                     /locus_tag="AC16VR_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018928949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR6010597.1"
                     /translation="MRWLTFLRDGRATFGYVTSNGQGVVDVGARSETADLRSAIAAGA
                     LADLAGSCGDEADVPLDRLTYLPTIPNPDKILCVGLNYKAHQEETGRGGEGFPTIFVR
                     FATAQIGHEQPMIRPTESATLDYEGEIALVIGIPGRRISRSDALDHVAGVSIYNDGSV
                     REYQRQTSQFTAGKNFVGTGGFGPWMMTPDEVGDLNSMEITTRLNGEVMQNATADLLV
                     HGFAELIEYCSTFIELLPGDVIVTGTPGGVGVARNPPVFMDEGDVVEVEVKPVGVLRH
                     RVVVG"
     gene            2353..>3046
                     /locus_tag="AC16VR_05260"
     CDS             2353..>3046
                     /locus_tag="AC16VR_05260"
                     /inference="COORDINATES: protein motif:HMM:NF024491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HIR6010598.1"
                     /translation="MPDRRGRSRILSGACWCLLILCVSGCVYLAQPEDAVVSQAQAPD
                     PQSRIGQLLGGGRGVPARGLLVEASDLALTARIDMIDAADATIDAQYFIWQNDPTGVL
                     VIQKLLDAADRGVRVRALLDDVQLTGFVDRLNALDDHPNIEIRIFNPFSVRFRYPLGF
                     IRIAEFAIDGARLNHRMHNKLMVADNQLAILGGRNIGDDYFGRNPKRNFVDTDILLSG
                     DIVPELSRGFDSY"
BASE COUNT          508 a          972 c         1038 g          528 t
ORIGIN      
        1 gtggggccgc tgccggcgga ttccgtgttc aaccgggcca tcgacgggga gttcgacgtg
       61 gtcctggcca tgtatcacga tcagggtcac atcgcgatca aggtgcacaa ctttcacgag
      121 agcaccacgg cgaccctggg catccccttc gtgcgaacct cggtggatca cggaacggcc
      181 ttcgacattg ccggctcggg ccgggccgac gagcgcggcc tggcggcggc gctggatgcg
      241 gcgagggcgt tgagccaggg tcagcttgcg cggctctagg gccgtggggc aaactcgcga
      301 ggcttaatcg aagcggcgtc gcgtccgcgt cacacagcag gggaggcagc atgaaggcgg
      361 cagtatttcg cgagatcaac cagcccatgg agatcgagga ggtggcgcta tccaaacccg
      421 gcccgcggga ggtcctcgtg cgcaccgccg cggcgggcgt ctgccacagc gacctgcact
      481 ttttcaacgg gacctatccc ggcctcttgc cgatggtgct gggccacgag agcgccggtg
      541 tggtggaggc ggtgggctcg gacgttcact acgtgaagcc cggcgaccac gtcattacct
      601 gcctttcggt cttctgcggg cattgcgagt tctgcctcac cggtcatctg tccctgtgcc
      661 aggagcccga gacccggcgc gctccggatc aggaaccgcg catcagccac gacggggcgc
      721 cggtgcagca gttcgccaac ctgggctcgt tcgcggagat gatgctcgtc cacgagcacg
      781 ccgtggcgaa gatccgcgag gacatgccgc tggaccgtgc cgcgctcatc ggctgcgggg
      841 tgaccaccgg tgtcggggcc gtcattcaca ccgccaaagt ggagccgggc tccacggtgg
      901 ccgtcatcgg ctgcggcggg gtgggattgt cctgcatcaa cggcgcggcc atcgcgggcg
      961 cgagccggat catcgcggtg gacatggtgg cctccaagct ggatctggcc cgcaagttcg
     1021 gtgcgaccga cgtggtcaac gccgcggaga tggatgccat caacgaggtg aaagcgctca
     1081 ccgggggcgg cgtgcactac tcctttgaag cgatcggcct gaaggcaacg gccgagcagg
     1141 cattcgggat gctgcgcaac ggcgggacgg ccaccgtcat cgggatgatt cctcccggtg
     1201 acatggtctc cctgcatggg cccgattttc tgttcgagaa gaagatccag ggctccatga
     1261 tggggtccaa ccggttccgg gtggacatgc cgcggttcgt ggatctctac ctgcagggca
     1321 agctgcatct ggacgatctg gtctccagcc gcatccggct ggaggacgtc aacgacgcca
     1381 tggcccagct cgagaccggc gagatcgccc gaaacgtgat cgtcttcgac tagggggaac
     1441 tatgcgctgg ctgacttttc tccgcgatgg gcgcgccacg ttcggctacg tgacctcgaa
     1501 cggccagggg gtggtcgatg tcggcgcccg cagcgaaacc gccgatctga gatcggccat
     1561 cgcggccggt gccctggccg atctggccgg ctcctgcggc gacgaggcgg acgtccccct
     1621 cgaccggctg acctacctgc cgaccatccc gaacccggac aagattctct gcgttgggct
     1681 gaactacaaa gcgcatcagg aggagacggg tcggggcggc gaaggattcc ccacgatctt
     1741 cgtgcgcttc gcgaccgccc agatcgggca cgagcagccg atgatccgtc cgaccgaatc
     1801 ggcaacgctg gactacgaag gggagatcgc cctcgtgatc ggaatacccg gtcgacgcat
     1861 atcccgctcg gatgccctcg atcacgtggc gggggtcagc atctacaatg atggcagcgt
     1921 gcgcgagtac cagcgtcaga cgtcccagtt caccgcaggc aagaactttg tcgggacggg
     1981 cgggttcggc ccctggatga tgacaccgga cgaggtcggc gatctgaaca gcatggagat
     2041 caccacgcgc ctgaacgggg aggtcatgca gaacgcaacg gccgatctgc tcgtgcacgg
     2101 gttcgcggag ctcatcgagt actgctccac cttcatcgag cttctgcccg gggacgtgat
     2161 cgtcaccggc acgccgggcg gggtgggtgt tgcccgcaac ccgccggttt tcatggacga
     2221 gggcgacgtg gtcgaggtgg aggtcaaacc cgttggcgtg ctgcggcatc gggtggttgt
     2281 gggctgagcc ggtcgccacc gcaccggcga gtggccgcgg tccatccgtg attctgactg
     2341 ctggccggac ccgtgcctga tcgtcgcggc cgctcgagaa ttctctccgg cgcctgctgg
     2401 tgcctgctca tcctctgcgt atccgggtgc gtctacctgg cgcagccgga ggatgccgtg
     2461 gtcagtcagg cccaggcgcc cgatccgcaa tcccggatcg gtcagcttct cggcggcggg
     2521 cgcggggtgc ccgctcgggg actgctggta gaggcctccg acctggcgct gaccgcccgc
     2581 atcgacatga tcgacgcggc ggacgcgacc atcgatgcgc agtacttcat ctggcagaac
     2641 gacccgacgg gcgttctggt aatccagaag ctgctggacg cggcggaccg gggggttcgg
     2701 gtacgcgcgc tgctagacga cgtgcagctc accgggttcg tcgaccggct gaacgcgctg
     2761 gacgaccatc cgaacatcga gatccggatc ttcaatccct tcagcgtgcg ctttcgctac
     2821 ccgctgggtt tcatccgcat cgccgagttc gccatcgacg gcgcgcggct gaatcatcgg
     2881 atgcacaaca agctcatggt cgccgacaac cagctggcca tcctcggcgg tcgcaacatt
     2941 ggcgacgact attttggcag gaacccgaag cgcaacttcg tcgacacgga catcctgttg
     3001 tccggcgaca tcgttccgga gctgtcccgg ggattcgatt cctact
//
LOCUS       DBNHQR010000226         2268 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1542113, whole genome shotgun sequence.
ACCESSION   DBNHQR010000226 DBNHQR010000000
VERSION     DBNHQR010000226.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1542113"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1866
                     /locus_tag="AC16VR_05265"
     CDS             <1..1866
                     /locus_tag="AC16VR_05265"
                     /inference="COORDINATES: protein motif:HMM:NF014805.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6010599.1"
                     /translation="PEIPVPQESVIDVVARVLSGVFSQQRPVVVAHRPKYTEVDLFRP
                     VRRVLIHARGCTAAKLIRIAQRNGIEVVLVQSDPDMESAAVDMLGEKDSVVCIGGNTP
                     DESYLNAMSVLSVAEHEGTDALHPGIGFLSENSQFAELVRSHGINFIGPPVSSMETMG
                     NKSNAINTALRLNVPVVPGSHGILTNVERAAEVAEEIGYPVLIKAVHGGGGKGIQVVE
                     SRDSFHELFHRVTVEARAAFGNGDVYLEKYVTSLRHIEAQLLRDVHGNTRVLGIRDCS
                     VQRDKQKVIEESDSTVLPDRLREEVLQHTAALADEVGYVGAGTVEFIYDLKSDAVYFM
                     EMNTRLQVEHPVTEWVSGVDIVSQQFRIAAGESIADLDPSPRGYSIEARVTAERVQAA
                     PGGELVFRPQPGQVAECSFPEDEGVEIIAAAAPGKFISPYYDSMIAQVVVYADDRAQA
                     IEKLAAYLRRVRVTGICTNIPLLKHILDDEVFQQGVYDTGYLPGLLARLDADELIAEI
                     EGGAGEVSGGIDMNAIRIDESDELKVLSPATAIFYGTPTPTEPPYVSVGDRVKLSDTL
                     CQLEAMKIFTPLSLADFNSEFELYEAGREYEITRINMGTGQQVNAGDLLFVVRPV"
     gene            complement(1885..>2268)
                     /locus_tag="AC16VR_05270"
     CDS             complement(1885..>2268)
                     /locus_tag="AC16VR_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010600.1"
                     /translation="ERPATAVACGATVTAAQLFAGASGPLLDVFYLNSSMDRYQVIAS
                     KAFTQTIGHGLKLAYYGTIIGVAETLPWWMFGAAMATAVAGTRVGTRLLGRLRDDVFR
                     RVSGWVILAIAAVCVVKGGMELAGW"
BASE COUNT          409 a          723 c          737 g          399 t
ORIGIN      
        1 cctgagatac ccgttcccca ggagtccgtc atcgacgtgg tcgcgcgcgt gctctcgggc
       61 gtgttcagcc agcagcgtcc ggtggtggtg gcgcaccggc ccaagtacac ggaggtcgat
      121 cttttccggc ccgtgcgccg tgtgctcatt catgcccgcg ggtgcacggc cgccaagctg
      181 atccgtattg cccagcgtaa cggcatcgag gtcgtgctgg tgcagtcgga cccggacatg
      241 gaatccgccg cggtggacat gctgggcgaa aaggactcgg tggtctgcat tggtggcaac
      301 acccccgacg agagctacct gaacgccatg agcgtcctga gcgtggccga gcatgagggc
      361 accgacgcgc tgcatccggg catcggtttt ctttccgaga actcccagtt cgccgagctg
      421 gtgcgttccc acgggatcaa cttcatcggg cctccggtgt ccagcatgga gaccatgggc
      481 aacaagtcca atgccatcaa caccgcgctg cgcctaaacg tgccggtggt ccccggaagc
      541 cacgggatcc tcaccaacgt cgagagggcg gccgaggtgg cggaggagat cggatacccc
      601 gtgctcatca aggccgtgca tggcggaggc ggtaagggca tccaggtggt ggagtcgcgc
      661 gattctttcc acgagctctt ccaccgggtc acggtagaag cgcgcgcggc gtttggaaac
      721 ggcgatgttt acctggagaa gtacgtcacc tctctaaggc acatcgaggc ccagctgctc
      781 agggacgtgc acggcaatac ccgcgtgctc ggcatccgcg actgcagcgt tcagcgcgac
      841 aagcagaagg tgatcgagga gtccgactcc acggtgttgc ccgatcgcct tcgcgaggag
      901 gtcctgcagc acacggcggc cctggcggac gaagtggggt atgtgggtgc aggcaccgtc
      961 gagttcatct acgacctgaa gtccgatgcc gtgtatttca tggagatgaa cactcggttg
     1021 caggttgagc atccggtgac ggagtgggtc tccggcgtcg acatcgtttc ccagcagttt
     1081 cgcattgccg ccggggaatc catagcggat ctcgatccgt caccccgcgg gtactccatc
     1141 gaggcgcgcg tgacggccga gcgcgtccag gccgctcccg gcggcgagct ggtcttccgc
     1201 ccgcagcccg ggcaggtggc cgagtgcagc tttccggagg acgaaggggt cgagatcatc
     1261 gccgctgcag cgcccggcaa gttcatctcc ccctactacg acagcatgat tgctcaggtg
     1321 gtcgtctacg cggatgaccg tgcccaggcc attgagaagc tggcggccta tctgcgccgc
     1381 gtcagggtta cggggatctg caccaacatt cccctgctca agcacatcct cgatgacgag
     1441 gtctttcagc agggcgtgta tgacacgggc tacctgccgg ggcttctcgc ccgcctggat
     1501 gccgacgagc tcatcgcgga aatcgagggc ggtgccgggg aggtttccgg cggcatcgac
     1561 atgaatgcca tacgcatcga cgaaagcgac gagctcaagg tgctctcgcc cgcgacggcc
     1621 attttctacg gaacgcccac gcccaccgag ccgccctacg tgtcggttgg ggaccgggta
     1681 aagctgtcgg acaccctctg tcagctcgaa gccatgaaga tattcacgcc cctttcgctg
     1741 gccgatttca actccgagtt cgaactctat gaagcgggcc gggagtacga gatcacccga
     1801 atcaacatgg gcaccggaca acaggtgaac gcgggagatc tgctgttcgt ggtgcggccc
     1861 gtttagggag ggcacgggca gcgctcacca gccggccagt tccatcccgc ccttgacgac
     1921 gcagacggcg gcgattgcca ggatcaccca tccactcacg cggcggaaca catcatctcg
     1981 caggcggccc agcagccggg ttccgacgcg cgtgccggcc acggccgtcg ccatggcggc
     2041 gccgaacatc caccagggca gggtctcggc aacgccgatg atggtcccgt agtaagcgag
     2101 cttcagcccg tggccgatgg tctgagtgaa cgccttgctg gcgatcacct ggtagcggtc
     2161 catggacgag ttcaggtaga agacgtccag cagcggtccc gatgccccgg cgaatagttg
     2221 cgccgcggta accgttgcac cgcatgccac cgccgtcgcc ggcctttc
//
LOCUS       DBNHQR010000227         4474 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_275697, whole genome shotgun sequence.
ACCESSION   DBNHQR010000227 DBNHQR010000000
VERSION     DBNHQR010000227.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4474)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_275697"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="AC16VR_05275"
     CDS             complement(<1..580)
                     /locus_tag="AC16VR_05275"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HIR6010601.1"
                     /translation="MLLCLDVGNSQIYCGVYDGDRLTVQFRRTSTVRSSSDELGVFLR
                     GALRENGVSPDGITQIAICSVVPDVLYSLRSCCQKYFGLEPLIMRPGIKTGLKISYKD
                     PKEVGADRIADAIGAIKLYPNRNLIVVDFGTATTVCAISRDREFLGGNIVPGVRLAMD
                     ALEEKTAQLPSVEIFPVTSAIGRSTVESIQNGL"
     gene            complement(595..1371)
                     /gene="panC"
                     /locus_tag="AC16VR_05280"
     CDS             complement(595..1371)
                     /gene="panC"
                     /locus_tag="AC16VR_05280"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF014611.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HIR6010602.1"
                     /translation="MKVLSSLGDWRAFRAGLVGEIGFVPTMGALHEGHAQLLQRSREA
                     NDHTVVSIYVNPTQFNNPADLDSYPGTLGEDLCRARELGVNAVLIPSYDELYPDDFRF
                     RVEEHEFSRELCGAHRPGHFTGVMTVVLKLLNLVRPRRAYFGEKDYQQYLLIRDMCDA
                     FFVDVEIVPCATVREEDGLALSSRNALLDEKGRARAGRLNRLLRDAPSDAAASRALEQ
                     AGFEVDYVVTKADRRFAAATLRCGEREVRLIDNVAIAQHG"
     gene            complement(1368..2156)
                     /gene="panB"
                     /locus_tag="AC16VR_05285"
     CDS             complement(1368..2156)
                     /gene="panB"
                     /locus_tag="AC16VR_05285"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF001452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HIR6010603.1"
                     /translation="MNVLDFPARKLNREKISMVTCYDFWSAQILEQTEVDCLLVGDSL
                     AMVMHGFDSTVHADTALMALHVAAVRRGAPSKFIVADMPFLAARKGLQPAMDAVQTLM
                     QAGANAVKIEGEAGQAELMTHIVQSGVPVMGHLGLTPQSVHTIGGHKVQGRTDEAAEQ
                     LRSSAEALAAAGCFALVLECVPSRLAAKVTRAIDIPTIGIGAGPETDGQVLVLQDLLG
                     MNPDFKPKFLRHYANGFESVSGAVNRFHADTQRGCFPSKSESYA"
     gene            complement(2445..3788)
                     /locus_tag="AC16VR_05290"
     CDS             complement(2445..3788)
                     /locus_tag="AC16VR_05290"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012503.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent decarboxylase
                     family protein"
                     /protein_id="HIR6010604.1"
                     /translation="MMWNDSESEIFAQMAGRLAAAFADLPASSAGLLDRDAVEVLRET
                     AQRLADNYPYFHPLYAGQMLKPPHPVARLAYGLAMLINPNNHALDGGRASSEMERAAV
                     AELAAMFGWQTHLGHLTSGGTMANLEALWIAGALQPGGVVLASEQAHYTHERLSGVLR
                     LPFEPIAADASGKMRMDALEDRLRRGGVGTVVATVGTTGFGAVDPLADIVELRHRYGF
                     RLHADAAYGGYFVLAGNLQAETRRHFDAMGAVDSIVVDPHKHGLQPYGCGSVLFRDPA
                     VGAFYKHDSPYTYFTSDALHLGEISLECSRAGAAAVALWATHRLLPPRPGGEFARGLE
                     RCRDAAEALHGRLAGHHRFLPLVQPELDIVAWAVLDADAEEASAAGRRLFEEAGELGL
                     HLALVQAPGSMIAHRWPEGADIPEGPVTCLRSVLMKPEHLDWLDRIWSLLEDLAG"
     gene            4034..>4474
                     /locus_tag="AC16VR_05295"
     CDS             4034..>4474
                     /locus_tag="AC16VR_05295"
                     /inference="COORDINATES: protein motif:HMM:NF014790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6010605.1"
                     /translation="MNYNLTEDQLAIKDLAERFTAEQITPNAAEWDEKHIFPRETIRA
                     AAELGFGGIYVSEASEGIGLGRIEAALIIEAMAYGCPSTSAFISIHNMAAWMIDQFGS
                     DDVRKRFVPDLVTMEKAASYCLTEPGAGSDAASLKTRAVRDGDHY"
BASE COUNT          873 a         1498 c         1349 g          754 t
ORIGIN      
        1 aaagaccgtt ctgaatgctc tccaccgtgg agcgcccgat agccgaggtc accgggaata
       61 tctccacgct aggcagctgc gcggttttct cttccagggc atccattgcc agtcggacgc
      121 ccggcacgat gttgcctccg agaaactcac ggtcccggga gatggcgcag acggtggtcg
      181 cagtcccaaa atccaccacg atgaggttcc gattcggata gagcttgatg gcgccaatgg
      241 catcggcgat ccggtccgcg cccacctcct ttggatcctt gtaggagatc ttgaggcccg
      301 tcttgatgcc cgggcgcatg atcagcggct cgaggccaaa gtatttctgg cagcaggagc
      361 gcagcgagta gagaacgtcg ggcacgacgg agcagatggc gatctgagtg atcccgtcgg
      421 gtgacacgcc gttttctcgc agcgcgccgc gcagaaacac tccgagctcg tccgaggagc
      481 tacgcaccgt agaggttcgt cgaaactgca cggtgaggcg gtcgccatcg tagacgccgc
      541 agtagatctg gctgttaccc acatccaaac agagcagcat gcctcctcct cggcctagcc
      601 gtgctgcgcg atcgcaacgt tgtcgatcag tcggacttct ctttcgccgc agcgcaaagt
      661 ggcggcggcg aatcgccgat cggctttggt gaccacgtag tccacctcga atcccgcctg
      721 ctcgagagcc cgggaagcag cagcatcgct cggcgcgtcg cgcagcagcc gattcagccg
      781 accagcacgc gcgcggcctt tctcgtccag caacgcgttt cgggaactca gcgccagccc
      841 gtcctcctcg cgtaccgtgg cgcatggcac gatttcgaca tccacgaaga acgcatcgca
      901 catgtcacgt atcagaagat actgctgata atccttttcg ccgaagtacg cgcgccgcgg
      961 ccggacaagg ttgaggagct tgagcaccac ggtcatcacg ccagtgaagt gccccggacg
     1021 atgcgccccg caaagctcac ggctgaactc gtgttcctcg actcgaaacc ggaagtcatc
     1081 gggatagagc tcgtcgtagc tcggtatcag caccgcgttc acgccgagct cccgggcgcg
     1141 gcaaaggtcc tcgccgagcg tcccagggta gctgtcgaga tcggcgggat tgttgaactg
     1201 agtggggttc acatagatgc tcacaaccgt gtggtcgttc gcttcccgac tgcgctggag
     1261 cagctgggca tgaccttcat ggagcgcgcc catggtcggc acgaacccga tctcgccgac
     1321 gagcccggcg cggaacgccc gccaatcccc gaggctggag aggactttca cgcgtaggac
     1381 tccgatttcg acggaaagca gccgcgctgg gtgtcggcgt gaaaccggtt gacggcgccg
     1441 ctcaccgact cgaacccgtt ggcgtaatga cgcagaaact tgggcttgaa atcgggattc
     1501 atgccgagca gatcctgcag aacgagcacc tgcccatccg tttccggtcc ggctccaata
     1561 ccgatcgtcg gaatgtcgat ggcccgggtg acctttgcgg cgagccgcga gggcacgcac
     1621 tcgagaacca gcgcaaagca accggccgcg gccagcgcct cggcagagga ccgcagctgc
     1681 tcggcggcct cgtcggtccg gccctgcacc ttgtgcccgc cgatggtgtg taccgactga
     1741 ggggtgagcc cgaggtgtcc catgaccgga acgcccgact gcacgatgtg cgtcatcagc
     1801 tcggcctgcc ccgcttcgcc ctcgatcttc acggcgtttg cgccggcctg catcagcgtc
     1861 tggacggcgt ccatcgcggg ctgcagcccc ttgcgggcgg caagaaacgg catgtcggcg
     1921 acgatgaatt tcgacggcgc gccccgacgc acggcggcga cgtgcaacgc catcagcgcg
     1981 gtatccgcat gcaccgtcga gtcgaatccg tgcatgacca tcgccaggct gtcaccgacc
     2041 agcagacaat ccacctcggt ctgctccagg atctgagcag accagaagtc ataacaggtg
     2101 accatcgaga ttttttctcg attaagtttg cgcgcgggaa aatcaagcac attcactgcg
     2161 gggccctccg gttgcgatca ccgatgcagt acccgcacga gcggcgcacg ccgttctgct
     2221 tcaggtacct gtcctttcat cgggatcaag tccaattgcc tatggcggtt caaatctctg
     2281 cctggcgccg acgtcaatgc ggggcgccac ggtctccgtg ctgagcatcg gagccgcaga
     2341 aaaccctacg tcactctcac aggaaagtcc atccgctccg gtgaacgcgc aacgggcgca
     2401 aaatgagacc caaaacccct ttctccttcc gcgtgaatgg agtttcagcc ggccagatcc
     2461 tccagcagtg accagattcg gtccagccaa tccagatgtt ccggcttcat gagcaccgac
     2521 cgcagacagg tcaccggccc ctcgggaatg tccgcgccct cgggccagcg gtgagcaatc
     2581 atcgaccccg gcgcctggac cagcgccagg tgcagcccga gctcaccagc ttcctcaaac
     2641 aggcgccggc ccgccgcgct ggcctcctcg gcatctgcat ccaacaccgc ccatgcgacg
     2701 atgtcgagct cgggctgaac gaggggcagg aacctgtggt gaccggcgag tcggccgtgg
     2761 agagcttcag cggcgtcgcg acagcgctcg agcccccggg cgaactcgcc acccggcctc
     2821 gggggcagca accgatgggt tgcccacagc gcgacggccg ccgcccccgc ccgcgagcat
     2881 tccaggctta tctccccgag atgcagcgcg tcggaggtga agtacgtgta aggcgagtca
     2941 tgcttgtaaa acgcgcccac cgccggatcg cgaaacagca cgctgccgca gccgtaaggc
     3001 tgcagtccat gcttgtgcgg atccaccacg atggaatcga ctgcacccat ggcatcgaaa
     3061 tgccgacggg tctctgcctg aaggttgccg gcgagaacga aatagccacc gtaagcggca
     3121 tcggcgtgca gccgaaaccc atagcggtgc cgtaattcaa cgatgtcggc gagaggatct
     3181 acggctccga agcccgtggt gccaacggta gcgaccaccg tacccacgcc accgcggcgg
     3241 agccgatcct ccagcgcatc catccgcatc ttacccgagg catctgctgc gatcggctcg
     3301 aacggcagcc tcagcacgcc gctcaggcgc tcatgggtat agtgcgcctg ctccgaggcg
     3361 agcacgaccc caccgggctg cagcgcgccg gcgatccaca gcgcttccag gttggccatc
     3421 gttccaccgc tggtgagatg tccgagatgg gtctgccagc cgaacatggc tgccagttcc
     3481 gccacggccg cccgctccat ctcggagctg gcgcggcctc catccagcgc gtggttgttc
     3541 ggattgatga gcatggcaag accgtatgcg agccgggcca ccggatgcgg cggcttcagc
     3601 atctgccccg cgtacagcgg gtggaagtac gggtagttgt ctgcgagacg ctgcgcggtc
     3661 tcccgcagaa cctcgaccgc atcacggtcg aggagcccgg cgctggaggc tggaaggtcg
     3721 gcaaatgctg cagcgagtcg accagccatc tgcgcgaata tctccgactc ggagtcgttc
     3781 cacatcatca gcctagctct ccattgccct gctcatgccg ccggatcagc atgggcgaga
     3841 ccacctaagg atgagggccc tgagcgctcg atcccccgac agctgcccct catcgacctc
     3901 ggacgatcct ccctgctagg gatatcgcgc gcaaccccgg ggcatctgaa cggttgcgcg
     3961 tcgcatcggt atctgagggc tgtgcaatgc attagaatcc cccgctgccc gcgacccgac
     4021 ggatccaaac ccgatgaact acaacctcac cgaagatcaa ctcgccatca aggatctggc
     4081 cgaacgcttc acggcggagc agattacgcc caacgcggcc gagtgggacg aaaagcacat
     4141 cttcccgcgg gaaaccatcc gcgcggccgc cgagctaggg ttcggaggaa tctacgtatc
     4201 ggaagcctcc gaaggcattg gccttggacg catcgaggcc gcactcatca tagaagcaat
     4261 ggcctacggc tgtccctcca catcggcttt catctcgatc cacaacatgg ccgcctggat
     4321 gatcgaccag ttcggctccg acgacgtcag aaagcgcttc gttcccgacc tggtaaccat
     4381 ggagaaggcg gcgagctact gcctcaccga accaggagcc gggtcggacg ccgcatcgct
     4441 gaaaacgaga gccgtgcgcg atggcgatca ctac
//
LOCUS       DBNHQR010000228         1612 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_275701, whole genome shotgun sequence.
ACCESSION   DBNHQR010000228 DBNHQR010000000
VERSION     DBNHQR010000228.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_275701"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..>1612
                     /locus_tag="AC16VR_05300"
     CDS             181..>1612
                     /locus_tag="AC16VR_05300"
                     /inference="COORDINATES: protein motif:HMM:NF004807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HIR6010606.1"
                     /translation="MKVAQAIAKALKAEGVEVLFAYPVNPIVEAAAAEDIRTVIVRQE
                     RVGLHMADAYSRLSSGDRIGVFAMQHGPGAENAFGGVAQAYSESVPILVLPAGYHRRI
                     ANYYPNFSSTLNMRNITKWAEPLTMGKAVPEVMRRAFFQLRNGRPGPVLVEIPVDVFR
                     EDVPDDWHHTPSFKTRSAPDPDAVATAAEVLAKAERPVIYAGQGVHYAKAWAELKALA
                     ETWNIPVTTSIEGKSAFPENHPLSLGSGGRANPRPVKAFLNEADVILGIGCSFALTGF
                     GVPMPAGRTIIHATLDPMDLNKDVPAQHGLIGDAALTLRALSQAMDGKDRSPAEARAG
                     VSGRIAAIREEWLGEWMPKLTNDEAPINPYRVLWELNQMVDKDNTVITHDAGSPRDQT
                     TPFWECTTPLSYIGWGKTTQLGYGLGLAMGAKLACPEKLCINVWGDAAIGMTGMDFET
                     CVRERIPILSILLNNFSMAIELPIMPV"
BASE COUNT          323 a          542 c          516 g          231 t
ORIGIN      
        1 cttcgggttc acagccgacc agagcagaac cgccataaac ggggccgccc agagcacgat
       61 gccccgctgc gatgggtagt cggccaacta caacctcata cgagtgagct agaatccgca
      121 agatacaaga gaaacggccc ggcggtaagc cgggcgccag ccaggatggg aggtgaccag
      181 gtgaaagttg ctcaggccat tgccaaagcg ctcaaggccg agggcgtgga ggtgctgttc
      241 gcatatccgg tgaacccgat cgtcgaggcg gcggcggcgg aggacatccg cacggtcatc
      301 gtccgtcagg agcgggtggg gctgcacatg gcggacgcct actcacgcct gtcgtcagga
      361 gacaggatcg gcgtgttcgc gatgcagcac gggcccggcg ccgagaacgc ctttggcggc
      421 gtcgcccagg cctactcgga atcggtgccg atcctggtgc tacccgcggg ctatcaccgc
      481 cgcatcgcca actactatcc caatttcagc tcgacgctga acatgcgcaa catcaccaag
      541 tgggccgagc ccctgaccat gggcaaagcg gtccccgagg tgatgcgccg cgccttcttc
      601 cagctgcgca acggaaggcc cggccccgtg ctcgtcgaaa ttcccgtgga cgtgttccgg
      661 gaggacgtac ccgatgactg gcaccacacg ccgagcttca aaacccgcag cgcgccggac
      721 cccgacgccg tcgccacggc agcggaggtg ctggccaagg ccgaaaggcc cgtcatctac
      781 gcaggtcagg gcgttcacta cgccaaggcc tgggcggagc tcaaagcgct cgccgagacc
      841 tggaacattc cggtaaccac cagcatcgag ggcaagagcg cgtttcccga gaaccacccc
      901 ctctcgctgg gctccggcgg cagggccaac ccgcggcccg tgaaagcgtt tctcaacgaa
      961 gccgacgtga ttctgggaat cggctgcagc ttcgccctca cgggcttcgg cgtgccgatg
     1021 cccgcgggca gaaccatcat tcacgccacc ctggacccca tggatctcaa caaggacgtg
     1081 cccgcgcagc acgggctcat cggcgacgcc gcgttgaccc tgcgcgcgct cagccaggcc
     1141 atggacggca aggaccgcag ccccgcggaa gcgcgcgccg gcgtgagcgg gcgcattgcg
     1201 gccatacggg aggagtggct gggcgagtgg atgccgaagc tcaccaacga cgaggccccc
     1261 atcaacccct accgggtgct gtgggagctc aaccagatgg tggacaaaga caacacagtg
     1321 atcacccacg acgcgggcag ccccagagac cagaccacgc ccttctggga atgtacgacc
     1381 ccgctgagct atatcggatg gggcaagacc acccagctcg gctacggcct tggcctggct
     1441 atgggagcca agctcgcgtg ccccgaaaag ctgtgcatca acgtctgggg cgacgccgcg
     1501 atcggcatga cgggcatgga cttcgagacc tgcgtgcgcg agcgcatccc gatcctgtcg
     1561 atcctgttga acaacttctc gatggcgatc gagctgccga tcatgccggt ct
//
LOCUS       DBNHQR010000229         2483 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_846189, whole genome shotgun sequence.
ACCESSION   DBNHQR010000229 DBNHQR010000000
VERSION     DBNHQR010000229.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_846189"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..1662)
                     /locus_tag="AC16VR_05305"
     CDS             complement(145..1662)
                     /locus_tag="AC16VR_05305"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008030174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004065 - arylsulfatase activity [Evidence
                     IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HIR6010607.1"
                     /translation="MITRIAATTAALLLSFAAWGAEQPNILVIWGDDIGITNISAYSD
                     GIMGYRTPNIDRIASEGVRFTDYYGDQSCTAGRSTFITGQSGLRTGLTKVGLPGAELG
                     IQDRDVTLAEVLRDMGYATGQFGKNHLGDKDEHLPTNHGFDEFLGNLYHLNAEEEPED
                     PDYPKVPGFRERFGPRGVIHSFADGKVEDTGPLTRKRMETADEEFVAAAKDFVDRSVK
                     ADKPFFVWLNTTGMHFRTHPAEKHLGKSGQGPYNDVMMAHDELVGQMLNQLDALGIAE
                     NTIVFYSTDNGVHFNTWPDAGVTPFRSEKNTNWEGAYRVPAMVRWPGKIAPGQVSNEI
                     MSHLDWMPTLVAAAGDVGLKDKLLEGTRIGRKQSKLHLDGYNFLPYLTGQAENGPRRE
                     FHYLNDEGMPVGVRVGDWKIVFAENRGKTMALWAEPFVTLRMFKIYNLRRDPFERAEH
                     NSNSYWDWMIDKAPQGYLGLAATAQFLQSFQAYPPSQRPDSWSIDRLTEALLEQQ"
     gene            complement(1797..>2483)
                     /locus_tag="AC16VR_05310"
     CDS             complement(1797..>2483)
                     /locus_tag="AC16VR_05310"
                     /inference="COORDINATES: protein motif:HMM:NF024242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR6010608.1"
                     /translation="RVSNRDSLKLEEVREGLRIHHDSGLPPSLGTHQSDLHFIEITLG
                     KLREALGPSWTPGRVGIAHSPSEPVPADSLLASAGVLTKSGTAFIEVSKAELRAVFPR
                     RRRPTNSRPPSVSLSSLPADFVGLARAQIEMLLPTGRIGIELLAESMGLSVRSVQRRI
                     ASAESSFSELLEDARFRLATSWLEHTSKSVTEIAFDLGYQYPANFTRAFRARTGLPPN
                     AYRATFSGSA"
BASE COUNT          389 a          764 c          835 g          495 t
ORIGIN      
        1 ccctcggggt gtcgcgcccg tacgggtcgg caggggcggc gtccgggggc agatcggcct
       61 gggcgatcgg ttccgcttct ccccaggccg cgccgctgat ccagcagatg cacaggaggc
      121 ccccggcgat ggcgctgcgg acgttcattg ctgctcgaga agcgcttcgg tcagccggtc
      181 gatggaccag ctgtccggcc gctggctcgg cgggtacgcc tgaaaggact gcaggaactg
      241 cgccgtggcg gcgaggccca ggtagccctg aggcgccttg tcgatcatcc agtcccagta
      301 ggagttcgag ttgtgctcgg cacgctcgaa gggatcacgg cgcaggttgt agatcttgaa
      361 cattctgagc gtgacgaacg gctctgccca cagcgccatc gtcttgccgc ggttctcggc
      421 gaagacgatc ttccagtcgc cgacgcgcac gccgacgggc atgccctcgt cgtttaagta
      481 gtggaactcc cggcgcggcc cgttttcggc ctggccggtc agatagggca gaaagttata
      541 gccgtccaga tgcagcttcg actgcttacg gccgatccgc gtgccctcga gaagcttgtc
      601 cttgagcccg acatccccgg ctgctgccac gagcgtcggc atccaatcca ggtgggacat
      661 gatctcgttg gagacctgac cgggagcgat cttgccgggc cagcgcacca tggccggcac
      721 gcggtaggcg ccttcccagt tggtgttctt ttcgcttcgg aacggggtga ccccggcgtc
      781 cggccaggtg ttgaaatgca cgccgttgtc cgtggagtag aagacgatgg tgttctcggc
      841 gatgcccagc gcgtccagct ggttcagcat ctgcccgaca agctcgtcgt gggccatcat
      901 gacgtcgttg tacgggccct ggcccgactt gcccaggtgc ttctccgccg ggtgggtgcg
      961 gaagtgcatg cccgtggtgt tgagccagac gaagaacggc ttgtcggcct tgacggaccg
     1021 gtcgacgaaa tccttcgcgg cggcaacgaa ttcctcgtcc gccgtttcca tgcgcttgcg
     1081 cgtgagcggc ccggtatcct ctaccttccc atcggcgaac gagtggatca cgccgcgcgg
     1141 cccgaaccgt tcccggaagc cggggacctt tggatagtcg ggatcctcag gctcctcctc
     1201 ggcgttcagg tgatagaggt tgcccaggaa ctcgtcaaag ccgtgattgg tgggcagatg
     1261 ctcatctttg tcgcccaggt ggttcttgcc gaactgcccg gtggcgtagc ccatgtccct
     1321 gagcacctcg gccagcgtca cgtctcggtc ctgaatgccc agctcggcgc cgggcaggcc
     1381 caccttggtc agccccgttc gcaggcccga ctggccagtg atgaaagtgg accggcctgc
     1441 cgtgcagctc tgatcgccgt agtagtcggt gaagcgcacg ccctcgctgg cgatacggtc
     1501 gatgttcggc gtgcggtacc ccatgatgcc gtcgctgtac gcgctgatgt tggtgatgcc
     1561 gatgtcgtcg ccccagatca cgaggatgtt cggctgctcg gcgccccagg cggcgaagct
     1621 cagcagcaat gcggcggtgg tcgccgctat ccgtgtgatc atcatgtttt cccctgcgcg
     1681 tgccgcttcg agcgtgatgc ggaaatcgct ttgcccgcaa cgaagtttgc gatgctacct
     1741 ccattcgagg gggcgatgaa gcgcgggggt caggtcgggc gatgccgctt cgaaagtcag
     1801 gcgcttccgg agaaggttgc tcgataggca ttcggcggca accccgttct cgctcggaac
     1861 gctcgagtga agttggcagg gtattgatat cccaggtcga aggcgatctc ggtgaccgat
     1921 ttggaagtgt gctccagcca gctagttgcg agtcgaaatc gagcgtcctc cagcagctcg
     1981 gagaaactcg attccgcgct tgcgatgcga cgctgcacgc tgcgaaccga caaccccatg
     2041 ctctccgcca gcagctcgat gccgatgcgt ccggtcggca gcagcatttc gatctgcgct
     2101 cgggccagcc cgacgaagtc cgcgggaagg ctcgagaggc tgacgctggg cgggcggctg
     2161 ttggttggtc tgcgtctgcg cgggaaaacg gcgcgtagct cggccttgct gacttcgatg
     2221 aatgccgttc cgcttttggt aaggacaccg gcgcttgcga gcagcgaatc cgcgggcacg
     2281 ggttcgctgg ggctgtgcgc gatgcccaca cggcctgggg tccaggaagg tccgagggct
     2341 tcccggagct tgccgagagt aatctcgatg aagtgcagat cgctctgatg ggttcctaaa
     2401 cttggcggga ggccggagtc gtgatgtatc cgcaggccct cgcggacctc ttcgagcttg
     2461 agggagtccc ggttgcttac tct
//
LOCUS       DBNHQR010000230         3365 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_592736, whole genome shotgun sequence.
ACCESSION   DBNHQR010000230 DBNHQR010000000
VERSION     DBNHQR010000230.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_592736"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1288)
                     /locus_tag="AC16VR_05315"
     CDS             complement(<1..1288)
                     /locus_tag="AC16VR_05315"
                     /inference="COORDINATES: protein motif:HMM:NF014740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HIR6010609.1"
                     /translation="MSRRKRQAIAVVTDFAVLPIALWTAIALRLGQVNPEVAQFWPAF
                     VVSSLLCIPVFGALGLYRHVVRHTGSEAIWAIVKGATIIALVVAAVAYMIPLPGFPRS
                     VPIIFWLLTLLYVSGTRFSMRAYFQWLQNRVRLRSPAVIYGAGSKGVELARVLSQQGD
                     YLPIAFIDDDRALHKRVIDGLNVYPPKSLGRVLRDSGARDVMVAIGSAEPDERRRIIE
                     FLEPFPARVRLIPDLVELESRRQTIANIRDVEIEDLIGRNTVSPLPHLLRGSVEGRSV
                     LVTGAGGSIGSELCRQIVRQGPQRMVLLDQSEFGLFEIQRELTQLIVSEGLPVDVVAV
                     LGSVTNGTLMRRNMESYAVETVYHAAAYKHVDLVEHNIIQGLRNNTFGTLYTAQAAMD
                     AGVREFILISTDKAVRTTSVMGASKRLAEMVLQALQE"
     gene            complement(1317..2300)
                     /locus_tag="AC16VR_05320"
     CDS             complement(1317..2300)
                     /locus_tag="AC16VR_05320"
                     /inference="COORDINATES: protein motif:HMM:NF013146.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HIR6010610.1"
                     /translation="MVSGTATGYLIAGFAASTVLVFLMLRVGRALNLMAVPNARSSHV
                     VPTPTAGGLGFVIPLLVFFGMLYLDGVDVALGLLLSLVLVAAASLWDDFAELSAGLRF
                     TCQTAAVALIVWQLDLPWAWYLLGLTGLALLWHVNLFNFMDGIDGIAAGQCLLYVVGV
                     QVLTLGAPGWMGELCWLTAGAMLGFLAYNWPPAKIFMGDVGSAVLGLLLALLALLLAR
                     DGVLPLIASLILLAGFWFDATYTLCVRMATGQAFTQAHRSHLYQKLAAFKGHLWTTGV
                     FIAFGLFWLLPLAWLTLRVADLAWVWLVAAVVPIGVASMRFGAGLPAQDEG"
     gene            complement(2270..3199)
                     /locus_tag="AC16VR_05325"
     CDS             complement(2270..3199)
                     /locus_tag="AC16VR_05325"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR6010611.1"
                     /translation="MRRAVVTGAGGFIGTHLCRALRHEGWEVAVITRAHALPGCERLP
                     VTLASADVELSRALGGAQVVFHLAGLAHGRASGAAEDELHRVNVESSARLLAASEAAG
                     VPAMIWLSSIKVLGDTSERPLRPEDAYRAGDAYARSKVEGERALLDREGFTRAAIVRP
                     PLVYGPGVAGNFASLARWVGSGVPLPLGAASAPRSMVGVDNLCSLMLRLAEEGSGVFH
                     VADARDRSVAQLIRAMAVLLGVPDRSWRVSPRLLAAVASALGQRARYTRLFEPLQVDQ
                     SMTRERLGWQPPHDPDQQLKETMRWFRERRQAT"
BASE COUNT          694 a         1132 c         1071 g          468 t
ORIGIN      
        1 tttcctgcag cgcctgaagg accatctcgg ccagccgctt gctcgcgccc atgacgctgg
       61 tggtgcgaac ggctttgtcg gtggagatga gaatgaactc gcgcacgccg gcgtccatgg
      121 ccgcctgcgc cgtgtaaagg gtgccgaagg tgttgtttct gagcccctgg atgatgttgt
      181 gctcgacgag atccacgtgc ttgtaagccg cggcgtggta aacggtttct acggcgtagc
      241 tctccatgtt gcgccgcatg agcgtgccgt tggtgacaga gccgagcacc gccacgacgt
      301 ccacgggcag cccttccgat acgatcagct gcgttagctc gcgctggatc tcgaacagcc
      361 cgaactcgga ctgatcgagc agcaccatgc gttgtgggcc ctgacgaacg atctgccgac
      421 acagctccga gccgatagac ccgccggctc cggtaacgag aacgctgcgc ccttccaccg
      481 aaccgcgaag aaggtgcggc aacggagata ccgtgttgcg accgatcagg tcctcgatct
      541 ccacgtcccg gatgttggcg atggtctgcc ggcgtgactc cagctccacg agatcgggaa
      601 tcaagcgcac gcgagcggga aacggctcca gaaactcgat gatgcggcgc cgctcgtccg
      661 gctccgcaga ccctatcgcc accatgacgt cgcgcgctcc ggaatcacgc agcacccttc
      721 cgagcgattt aggcgggtag acgttcaacc cgtcgatgac gcgcttgtgg agcgcacgat
      781 cgtcatcgat gaaggctatc gggaggtagt cgccctgctg gctcagcacc cgcgccagct
      841 ccacgccctt cgacccggct ccgtagatga cggcgggact gcgcaggcgg acccgattct
      901 gcagccattg gaagtaagcg cgcatggaga aacgggtgcc gctgacgtag aggagggtga
      961 gcagccagaa gatgatggga acggatcggg ggaatccggg cagcgggatc atgtaggcca
     1021 cggcggccac aaccagggca atgatggtcg cgcccttgac gatggcccag atcgcctcgc
     1081 tgcccgtatg gcggaccacg tggcgataca agccaagagc accgaaaacc gggatgcaca
     1141 gcaggctgct gacgacgaag gccggccaga actgcgccac ctccggattg acctgcccca
     1201 ggcgcagggc gatcgctgtc cagagcgcga tgggaagcac ggcaaagtcc gtgaccacgg
     1261 caatagcctg ccgcttgcgc cgggacaact gattgatgaa attctcgatg atcttctcag
     1321 ccctcgtcct gggcgggcag ccccgcgccg aaacgcatgg acgccacgcc gatgggaacc
     1381 acagcggcca caagccagac ccatgccaga tccgcaacgc gcaacgtcag ccaggcaagg
     1441 ggcagcaacc agaacaagcc gaaggcgatg aacacaccgg tagtccagag atggcccttg
     1501 aatgccgcaa gcttctgata caggtggctg cgatgcgcct gagtgaacgc ctgaccggtc
     1561 gccattctga cacacagcgt atacgttgcg tcaaaccaga acccagcaag cagtatgaga
     1621 gaggcgatga gcggcagcac gccgtcgcgc gccagaagga gcgcgagcag agcaagcagc
     1681 aggcccagca ccgcgctccc gacatctccc atgaagattt ttgccggcgg ccagttgtag
     1741 gccaagaatc ccagcatggc gccggccgta agccagcaaa gctcgcccat ccagccgggc
     1801 gctcccaggg tgaggacctg gacacccacc acgtaaagca ggcattgccc cgccgcgatc
     1861 ccgtcgatgc catccatgaa gttgaacagg ttgacgtgcc agagcagcgc cagccccgtc
     1921 aatcccagta gataccaggc ccagggcaga tcgagctgcc agacgatgag cgcaacggca
     1981 gccgtctgac aggtaaaccg cagaccggcc gaaagctcgg caaaatcgtc ccagaggctg
     2041 gccgccgcga cgagaaccag gctgagcagc aagccgaggg ccacgtcgac gccgtcaagg
     2101 taaagcatcc cgaaaaaaac caggagcggt atcacgaacc ccaacccccc cgccgtcggc
     2161 gtggggacga cgtgggagct gcgcgcattg ggaacggcca ttaggttcaa cgcccggccg
     2221 acccggagca tcaggaagac gagcaccgtc gaggccgcga accccgcgat caggtagcct
     2281 gtcgccgttc ccgaaaccat cgcatcgttt ccttcaactg ctgatcggga tcgtggggcg
     2341 gctgccagcc aaggcgctcc cgggtcatgc tctgatctac ctgcaggggc tcgaacagcc
     2401 gagtgtagcg tgcgcgctgc cccaatgccg aggcgacggc cgccagcagc cgcggcgaaa
     2461 cccgccacga gcggtcggga acccccagca gcaccgccat ggcacgaatc agctgggcga
     2521 cgctgcggtc gcgagcatcc gcaacgtgga acacgccgga gccttcctcg gcaagacgaa
     2581 gcatcaggct gcacagattg tcgaccccga ccatgctgcg cggcgcagac gccgctccga
     2641 gcggcaacgg gacgccgctg ccgacccacc gcgcgagact cgcgaagttt ccggccacgc
     2701 cgggcccgta aaccagaggt gggcgcacga tggccgcgcg cgtgaagccc tcccggtcca
     2761 gaagcgcgcg ttccccctcg actttgctgc gcgcgtaggc atccccagcc cgataggcat
     2821 cctctggtcg aagcggccgc tcgctcgtat ctccgagcac cttgatcgag ctcagccaga
     2881 tcatcgccgg caccccggcg gcttccgacg cggcgaggag gcgggcggaa gactcgacgt
     2941 ttacccgatg cagctcgtcc tcagccgcgc ccgatgcccg gccgtgagca agtccggcga
     3001 gatgaaacac cacctgtgcg ccgccgagcg cccgcgacag ctcgacgtcg gctgaggcca
     3061 gcgttacggg aagccgctcg catccgggga gagcgtgagc gcgggtaatg accgccacct
     3121 cccaaccctc atgacgaagc gcgcggcaca ggtgggtacc gatgaagccg ccggcgccgg
     3181 tgacgacggc tcttctcacg gcagcagcaa ctcgtcgtag acccggaacg tatgtcgaac
     3241 cacgtcctcg agggagaaga cctcctccgc cgcgctgcgc gccgcgcggg caaagcgatg
     3301 acgaagcgca tcgtcctcgc ccaggcgcag catggcgtcg gcaagcggct cgctgacccg
     3361 gggcg
//
LOCUS       DBNHQR010000231        14524 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1290258, whole genome shotgun sequence.
ACCESSION   DBNHQR010000231 DBNHQR010000000
VERSION     DBNHQR010000231.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 14524)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14524)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14524
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1290258"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..1216
                     /locus_tag="AC16VR_05330"
     CDS             161..1216
                     /locus_tag="AC16VR_05330"
                     /inference="COORDINATES: protein motif:HMM:NF024771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR6010612.1"
                     /translation="MAVPWPEAPLFAADVPELQDFLIVEVETASGMVGMGYLQPLAGG
                     LKTLACCVHEMLAPLLVGQEADDVEALWDRMWSATYIQGRMGITVMAMSALDIALWDL
                     VGKRAGEPLYRLWGGVDQHIPTYGSGCYRGLGQDGMIRKAERYAADGFSAIKMQVAHM
                     ATHDQDVENVAAMRAALGADIEIMVDVNQGWTAAEAIEVGSRLDESGIYWLEEPVIAH
                     DFAGYRQVARALKTRVVGGENHFTHHDLQPFFDDPCVPILQPDVMRGGLTELRRIAAM
                     AEPLGISIAPHLFPELMTHLLASIPNGSWLEYMGWHDELWAQPVVPSGGLTRPPDRPG
                     HGLAFRADVLAEYRVAG"
     gene            complement(1213..2439)
                     /locus_tag="AC16VR_05335"
     CDS             complement(1213..2439)
                     /locus_tag="AC16VR_05335"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6010613.1"
                     /translation="MHDDDKTNDDLGLGRNPTVTLLLAFFQVFLVIMPVAVPFFQSKG
                     LSMQEVFSLQALFALVVLLAEVPSGYVADIFGRKRTLIVGAAFCGLGHTLLLGADGFW
                     TLALFEISLGIGSSLISGADIALLYDTEVALGRGERQQRQVVGRLYSMRTFSEAVAGV
                     VCSVVLLWSMDLAVYVQALIGWLPLVFAFALVEPPGERMERSGHLVNMARICRYLVSH
                     SAVLRLTFLALCIWALTTFYAVWLLQKIWEAQGLGLIHFGYLWGFLSLVAALAGRWAY
                     RAEEMLGTTGVLVFIGLAPAAGYLCLDLFGVVGSVLAGLTFWISRGLGMVILRDALNR
                     RIPGEFRATANSLASFGFRGAFVLTGPLVGYAFDLWGMSVTLSLLALATLVIFAGLVL
                     PLVAAARSQRLEAQAT"
     gene            complement(2869..3831)
                     /locus_tag="AC16VR_05340"
     CDS             complement(2869..3831)
                     /locus_tag="AC16VR_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010614.1"
                     /translation="MSLERAYQFARRTNVLVLPAVLMGVEIAVFLAVSPFADSSEILA
                     SMSQAQFVGTLALFSIMPPYFLCMVTLQRLRTEESLAEMADLLEPTSAARVQGCIAGI
                     HPAGWFLVVLGAVFGGYQNEEFIRRVGASGTLNAMDVAFVLANCFVWAAIALVLSWRL
                     PISLALSRLGAQMKVDVYRLDRLRPLARIATLDILVVAGAMALMPLQSLDAEFRIYNY
                     NYGIIVGVPSAIVLFVLPLWGVRGNIGDRKADRVAVLQAKLDVVDRNDVAAVEALSAH
                     IERLRGLSNWPVDLRLATRIFAYVIIPPLAWVGAALVEILIDRL"
     gene            complement(3839..4732)
                     /locus_tag="AC16VR_05345"
     CDS             complement(3839..4732)
                     /locus_tag="AC16VR_05345"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014871071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIR6010615.1"
                     /translation="MSPEEVLSRPPRVLTRDQRESYFERGYAVFPGLVDDHWLQRLNA
                     VTDAFVEASREVVAGGDRRFDLEPDHCAAEPRIRRLNTPVELSETYWEFASRGPFADV
                     AEDLLGPSFKFHHSKLNFKWSGGGEEVKWHQDIQFWPHTNYDVLTLGVYLREVDAAMA
                     PMGVVPGSHAGPLYDLYDPSGRWTGNIRDEDLQSAGVENAEYLMGPAGSVTVHNCRSV
                     HGSAPNLSERPRPLLLCAYSAADAFPITTLTAGASHSEALIRGEPARWARFDPRPCLM
                     PPDWSRQGGYKSIFADQQKEA"
     gene            4878..6056
                     /locus_tag="AC16VR_05350"
     CDS             4878..6056
                     /locus_tag="AC16VR_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase-like domain-containing protein"
                     /protein_id="HIR6010616.1"
                     /translation="MSDIVYDFGSGRSDPGTFPTEALQRAAVTAIGKEAVTLTDYPGG
                     LGYPGLRRAMARRESEREGVDVNPDHLVITNGSMQAVTLTAEALQETAGDAIIVEEYS
                     YPGTLSAYRSLKFDMHGIPLSEQGMRLDRLEEKLVELDQAGRRPKFIYTITTYQNPTG
                     FVMPRANRLELIELARRYEIPVIEDNCYGDVHYEGPVEPALYALDDDPNQIYLCSLSK
                     ILAPGLRLGYLIARPPMLERILARRHDAGSNTLAAAIAAEFYRDGIWDHTRYANAALK
                     EKRDLLFDRLQAELDDVCVWSRPVGGLFVWVRIPDDINRKQLYELAQSRGFNYLPGIS
                     FHVETRNVPYLRLAFGHLTLDQIRDGIPVLAQCIRECRTSNEPRGFDSLFQDREGSSA
                     "
     gene            complement(6029..6190)
                     /locus_tag="AC16VR_05355"
     CDS             complement(6029..6190)
                     /locus_tag="AC16VR_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010617.1"
                     /translation="MEFRLSAAADGARLEYRHHVTGFGAESMAEPVDGVQLGQLERLK
                     RYAEEPSRS"
     gene            complement(6445..6831)
                     /locus_tag="AC16VR_05360"
     CDS             complement(6445..6831)
                     /locus_tag="AC16VR_05360"
                     /inference="COORDINATES: protein motif:HMM:NF024845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HIR6010618.1"
                     /translation="MECPKCPGTLETKTYGRKITVHRCSDCGGLWCKPEVLLEMKREW
                     MADVILDSGDPKLGQALDKLDNISCPECGVKMEKKTDDRQTHIWYESCPQCEGMFFDA
                     GEFTDLKYDTFMDRIRGALRGRRPSA"
     gene            complement(6843..7346)
                     /locus_tag="AC16VR_05365"
     CDS             complement(6843..7346)
                     /locus_tag="AC16VR_05365"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6010619.1"
                     /translation="MPALSIAERDDFLAERGIVMRIGVVRADGSPLVTPIWFLHEDGA
                     IFFTPREKSEWFACLRRDPRVSLCIDEQPLPYRKVIVEGRAELVHDVGEDDVWRDLYR
                     RIAKRYVDDAEAEAYVQNTIDQPRGLYRVVMSEGEIKTWRMPVAGEAPEGIWHSRYYK
                     PDTKFNR"
     gene            complement(7367..8617)
                     /locus_tag="AC16VR_05370"
     CDS             complement(7367..8617)
                     /locus_tag="AC16VR_05370"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6010620.1"
                     /translation="MNVKPVPTLDEEVNEIRLATAEIVNEDILPNEDRLWGWLADSAN
                     ATNEVKQEAKALRVDIQEKVKNARLWAPHLPREFGGMGLTFMQHAYMNEVLAYSPGAA
                     SLFGVVAPNSGNQKILVKYGTPDQHEKWLKPLTDGTAQSGFSMTEPHNPGSDPRSITT
                     RAELDGDEWVINGHKWFTSNGFAADFYIVMCRTSDPDDPGGANEKMTQIIVPRKTPGV
                     NIVRGVPVWGRASSHCEIVYDNVRVPKENQLGRTGTGHQAAQDRLGAGRVYHCMNSIG
                     SMWRAFDLMIDRLMTREVHGGEKLEDKQFMQGFVADSYIDIQTARLMTIHCAEKMESG
                     ADPRTDISAIKIFVPQAYHRVVDRAIQVWGAAGVSGDLPLASMYQGARTLRLADGPDE
                     VHRILIAKNILRRYHAGESWDFGN"
     gene            8773..9882
                     /locus_tag="AC16VR_05375"
     CDS             8773..9882
                     /locus_tag="AC16VR_05375"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6010621.1"
                     /translation="MGTHELNYPHAEHPAPGTTVELAPGVRWLTMPMGGSLNHINLYL
                     LEDTTGWWIVDTGLSLDQTRELWHEIFSRELQGRPVLGVICTHFHPDHIGQSKMITDE
                     FRCSLYMTRAEYYQARAFSGSGPTYQSSWLGETFYQRAGMPADYLEQLREMWAHRSSE
                     GMSMPDMPAGYRRLEDGDVLTIGGHDWRIVVGSGHSPEHACLYCAALKIMISGDQILP
                     VITSNVSVHPTEPEANPLKDWMESHDRFLRVPDDTLVLPAHNLPFYGVRERLRQLIDH
                     HEDRMLAIEEHCVEPAVAKDLLPVLFARELDPRQMMMALGEAIAHLHLLVHRNRIERT
                     LHEDGCYRFLSIDPDLKRRAHPGDHDAPSERPVLV"
     gene            9908..10186
                     /locus_tag="AC16VR_05380"
     CDS             9908..10186
                     /locus_tag="AC16VR_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010622.1"
                     /translation="MQKGKLKRRIADYTYRMMHWGQNRLPPGIRSLVGVLLMVGGVFG
                     FLPVLGFWMLPLGVAFVALDVPGTRRHIDMWMLRLYRQAYLDEPTSNQ"
     gene            complement(10156..11022)
                     /locus_tag="AC16VR_05385"
     CDS             complement(10156..11022)
                     /locus_tag="AC16VR_05385"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6010623.1"
                     /translation="MNHWMVGDVKITRVVEMEVAGGTRFILPDATRGACLPIEWLHPH
                     FMDEAGNLIMSIHALVVDTGSRRIIVDTCIGNDKQRSIPAWTNLQTSFLEDLGAAGYP
                     RETVDTVLCTHLHVDHVGWNTMLVDGEWVPTFPNARYLVAEKEFRYWDGEAEDEINQG
                     IMADSVRPIVAAGLMDFVDWEHQLCPEVALEPTPGHTPGHVSVHIRSNGKEALITGDC
                     IHHPCQMTRTDWCSSADYDRAAGEATRERLLESYVDSDVLIIGTHFATPTAGHVRRLD
                     GGGYWLDVGSSR"
     gene            11144..12964
                     /locus_tag="AC16VR_05390"
     CDS             11144..12964
                     /locus_tag="AC16VR_05390"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-acyl-CoA synthetase"
                     /protein_id="HIR6010624.1"
                     /translation="MGPIAKAVDVVRALSNVPTLLALKRGMKPRPVDEHDCFAANVER
                     NAAAFPDRTAIIFEGNSVTWAEFNAQCNRFASAFKAQGLRKGDAVSVMMENRIELLAT
                     IVALNKLGVVAGLINTNLRGRPLAHCITVTESRTCVFGEEVGEAVAEVKDELPLEEGS
                     GYLFVPDAGSGAAPNWAADLSRLAADADDADPPETGDVQLGDRALYIFTSGTTGLPKA
                     AVLSNRRFVATAALVHKAGLRCSEEDRIYICLPLYHGTGLLIGVGASFSSGASMFIRR
                     KFSASNFLREVREYQTSCLIYIGELCRYLMNTPAQPDDHDNPLVTMMGNGLRPDIWPA
                     FKSRFGIKRITEFYGASEGNVSFVNLLNKDCTIGMTGSKVALVRYDVDEDEIVRDENG
                     HCMEVAPGEPGLLLGHISEDAVFEGYTNAEATESKVVRNAFEDGDAWFNSGDLIRQVD
                     VGYSLGYPHYQFVDRVGDTFRWKSENVSTNEVGELINGFPDVKFCNVYGVEIPGADGR
                     AGMVAITLADGVTELDADALSRHVSDTLPPYARPVFVRVQPDIDVTGTFKMVKGDLRK
                     QGYDLDQITDPLFVMKPGESAYVPLDREFAGKIASGAAGY"
     gene            complement(12972..14036)
                     /locus_tag="AC16VR_05395"
     CDS             complement(12972..14036)
                     /locus_tag="AC16VR_05395"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6010625.1"
                     /translation="MELWTLTHSRADNVLAVARRAEAAGWAGVMVVDSQNLAADSYVG
                     LAVAATGTQRIGLGTGVTNTVTRHPAVTASAAMAVQDVSGGRMVLGIGRGDSALAHLG
                     RSPARLPGFERYLDVLQRYLKGEAVPFEDIPMDDHIAAPVADLDLADHPSASRIRWFK
                     DDVAKVPVEVAATGPKVIEMAARLADRVMFTLGAVEERLSWAMETAMAARTGAGIPDD
                     PAFGAYVNLVCHRDRAVARDLVRGGLTTFARFSVMHGQIAGPADDHDRATLTSLHDGY
                     DMKAHTRADSSQAALLDDDFVDRFAIAGPPDYCVERLNRLASMGIEKVAISGPTAGTD
                     PVEARRALALLDEAVVPQLA"
BASE COUNT         2691 a         4880 c         4517 g         2436 t
ORIGIN      
        1 tgggccttgc ggcgggcgag ctcaccgaga tcgtcaacac gccggcgcgg aagtacgagc
       61 gcaagcgatc gggccccctc ctccgtccgg gtctcatcga cgcggtcgag gtctaatcct
      121 gacccaacca gatcccatcg cggacatccg gaccacgcgg ctggccgtgc cctggccgga
      181 ggcacctctg ttcgcagccg acgtcccgga actccaggac tttctgatcg tcgaggtcga
      241 gaccgcgtcc ggcatggtgg gaatgggcta cctgcagccg ctggcgggcg ggctgaaaac
      301 cctggcctgc tgcgttcacg agatgcttgc acccctgctg gtcggccagg aggccgacga
      361 cgtcgaagcg ctgtgggacc gcatgtggag cgccacctac atccaggggc ggatgggcat
      421 caccgtgatg gccatgtcgg cgctggacat cgctctgtgg gaccttgtcg gcaagcgcgc
      481 gggcgagccc ctgtacagac tatggggcgg ggtcgaccag cacatcccga cttacgggag
      541 cggttgctac cgcggcctcg gccaggacgg gatgatccgc aaggccgagc gttacgcagc
      601 cgacggcttt tccgccatca agatgcaggt ggcccacatg gccacgcacg atcaggacgt
      661 ggagaacgtg gccgccatgc gcgcagcgct tggggcggac attgaaatca tggtggacgt
      721 caaccagggc tggacggcgg cggaagccat cgaggtcgga tcgcggctgg acgaatcggg
      781 catctactgg cttgaagagc cggtgatcgc ccacgatttt gccggctacc ggcaggtcgc
      841 ccgggccctg aagacacggg tggtcggtgg cgagaaccac ttcacccacc acgatcttca
      901 gcccttcttc gacgatccct gcgtgcccat cctgcagccc gacgtcatgc gcggcgggct
      961 gacggagctc aggcggatcg cggccatggc agagcccctc ggcatcagca tcgcgcccca
     1021 cctgttcccc gagctcatga cccaccttct cgccagcatt cccaacggga gctggctcga
     1081 gtacatgggc tggcacgacg agctgtgggc gcagccggtg gtcccgtccg gcgggcttac
     1141 gcgcccgccg gacaggcccg gacacgggct ggcgttcaga gccgacgtcc tcgccgagta
     1201 ccgcgtcgcc ggctaggtag cctgggcttc cagcctctgg ctgcgggcgg cggcaaccag
     1261 cggcagcacc agcccggcga atatgaccag cgtcgccagc gcgagcaggc tcagcgtgac
     1321 gctcatgccc cagaggtcga aggcgtagcc caccaggggc ccggtcagga cgaaggcgcc
     1381 ccggaagccg aagctcgcca gcgagttggc cgtcgcccga aactccccgg gaatccgccg
     1441 attgagcgca tcccgcagga tcaccatccc gagcccccga ctgatccaga acgtcagccc
     1501 ggcaagaacg ctgcccacca ctccgaacag gtctaggcaa aggtacccgg cggcgggcgc
     1561 caggccgatg aagaccagca cgcccgtggt gccgagcatc tcctcggccc ggtaagccca
     1621 ccgtccggcc agagcggcga ccaggctcag gaacccccag aggtaaccga agtgaatcag
     1681 tcccaggccc tgtgcctccc agatcttctg cagcagccag acggcgtaga acgtcgtcag
     1741 cgcccagata cacagcgcca ggaaggtcag ccggagcacg gcgctgtggc tcaccagata
     1801 gcgacagata cgggccatgt tcaccaggtg ccccgagcgc tccatacgct cgcccggggg
     1861 ctcgaccaga gcgaaggcga acaccagggg tagccagccg atcagcgcct gaacgtaaac
     1921 ggccaggtcc atggaccaca gcagcacaac gctgcagacg acgccggcca ctgcttccga
     1981 gaacgtccgc atcgagtaca gccgaccgac cacctgccgt tgctgacgct cgccgcgccc
     2041 cagcgccact tccgtgtcgt agagcaacgc gatgtcggcg cccgagatca ggctgctgcc
     2101 gatgcccagc gatatctcga acagcgccag cgtccagaac ccgtccgcgc cgaggagcag
     2161 cgtgtggccc agaccgcaga acgccgcgcc gacgatgagc gtgcgcttgc gaccgaagat
     2221 gtcggcgacg tagccggaag ggacctcggc caggagcacc accagggcaa acagagcctg
     2281 cagcgagaac acctcctgca tgctcagccc cttgctctga aagaacggca cagccaccgg
     2341 catgatgacc agaaacacct ggaagaatgc cagcagcagc gtcaccgtcg ggttgcggcc
     2401 caacccgaga tcgtcgttcg tcttgtcatc gtcgtgcatc ttcatccctc cgaccgttcg
     2461 ccgggacccg ccacggacgc cggaatccgg cgcccagaaa agaaaaaccc cgaaggcttg
     2521 cggctcttcg gggttttttt cggaactcgc tgctgcgttt cctaaagcat catccgctcg
     2581 agccgtgccc tcctcgcgca atacgcacca tgtggtccgc acacagggac tgcgcagaca
     2641 cacggccacc cgcgaaggtg gtagctggtt tgtttcgata cgcgttcatg atctcgagct
     2701 tctctgatcc ggctcacgcc gaaaaaacag ggcgcgcatg ttgcgccggg cgttctctgg
     2761 tgtcaacgga agtcagcaaa aaaaggggga atctgtgacc cctttcagca agccagcctc
     2821 cagacagctt cgagcatcgg ggtccgatcg ctggggttgg gcttgctgct agaggcgatc
     2881 gatcagaatc tcgaccagcg ccgcgcccac ccatgccaac ggcgggatga tcacgtacgc
     2941 gaagatgcgg gtggcaaggc gcagatcgac cggccagttg gaaagacccc gcaaacgttc
     3001 aatgtgtgcg ctcagggctt ctacggcggc tacgtcattg cggtccacca catccagctt
     3061 cgcctgcagc acggcaaccc gatcggcctt gcggtcgccg atgttcccgc gcacccccca
     3121 caggggcaga acgaacagca cgatggcaga cggcacaccc acgatgatcc cgtagttgta
     3181 gttatatatc cggaactcgg catccaggga ctgcagcggc atcaacgcca tggcgccggc
     3241 aaccaccagg atgtccagcg tggcgattcg ggccagcggc cgcagccgat ccagccggta
     3301 gacgtcaacc ttcatctgcg cgcccagtcg cgacagcgcc aggctgatcg gcaggcgcca
     3361 gctgagaact aacgcgatgg cggcccagac gaaacagttg gcaagcacga aggccacgtc
     3421 catggcgttg agcgtgccac ttgcgccgac tctgcggatg aactcctcat tctggtagcc
     3481 accgaacacc gccccaagga cgacgaggaa ccaacccgca ggatgaatgc ccgcgatgca
     3541 tccttggacc cgggcggcgc tcgtcggctc gagcaggtcg gccatctcgg caagggattc
     3601 ctccgtcctg agccgctgaa gggtgaccat gcagaggaaa tagggcggca ttatgctgaa
     3661 gagtgccagg gtgccaacga actgtgcctg gctcatgctg gcgagaattt cgctggaatc
     3721 ggcaaagggc gagaccgcga gaaagacggc gatctccacg cccatgagca cggccggcag
     3781 cacgagcacg ttcgtgcgcc tggcaaattg ataggctcgc tccaacgaca tcggcgcctc
     3841 aggcctcttt ctgctgatcg gcaaagatgg acttgtaacc tccctgcctg gaccagtcgg
     3901 gcggcatcag gcagggccgg ggatcgaatc gcgcccagcg ggccggctcc cccctgatca
     3961 gcgcttcgct gtgagaggca cccgccgtca gcgtggtgat gggaaacgcg tccgccgccg
     4021 agtaggcgca aagcagcagg gggcgcggcc gctcgctcaa attgggcgca gaaccgtgaa
     4081 cggagcggca gttgtgaacc gtgacgctac ccgccggccc catcagatac tccgcgttct
     4141 ccacgccggc gctctgcagg tcctcgtccc ggatattgcc cgtccaccgg ccggaggggt
     4201 cgtacagatc gtaaagagga cccgcgtggc tgcccggcac cacccccatg ggcgccatgg
     4261 ccgcgtcgac ttcccgaaga tatacaccca gggtcaggac gtcgtagttg gtgtgtggcc
     4321 agaactgaat gtcctgatgc cacttcacct cctccccgcc gccgctccat ttgaagttga
     4381 gcttggagtg atggaacttg aagctgggtc cgagcagatc ctcggcaaca tccgcgaacg
     4441 ggccgcggct ggcgaactcc cagtaggtct cgctgagctc cacgggcgtg ttcaggcgcc
     4501 gtatccgcgg ctcggccgcg cagtgatcgg gctccagatc gaaccgccgg tcgccgcccg
     4561 cgaccacctc ccggctcgcc tcgacgaaag cgtcggtcac cgcgttcaac cgttgcagcc
     4621 aatggtcatc taccaggccc gggaacacgg catagcctcg ctcgaaatag ctctcgcgct
     4681 ggtcccgtgt cagcacgcgg ggcggacggg aaagaacttc ctccgggctc atctcaagca
     4741 ctccactcct gttggccgtc ctgcctgcca gggcttgcgg ccgccgcgtg ttacgatacc
     4801 tcaacggccg gggcaattgg cgcccgatcg ttgccggcgg ctagactcgc aggccggacc
     4861 ggatcagggg acccatcatg agcgacatcg tctacgactt cggcagtggc cggtcggacc
     4921 ccggcacctt tcccaccgaa gcgctgcagc gcgcggcggt taccgccatc ggcaaggaag
     4981 ccgtcaccct caccgactac ccgggcgggc ttggctatcc cgggctgcgc cgggccatgg
     5041 cgcgccgcga atccgagcgc gagggcgtgg acgtgaatcc ggatcacctg gtcatcacca
     5101 acggatcgat gcaggcggtc acgttgacgg cggaggccct gcaggaaact gcgggcgacg
     5161 ccatcatcgt cgaggagtac agctaccccg gcaccctgtc cgcctatcga agcctgaaat
     5221 tcgacatgca cggcatcccg ctctcggagc aaggtatgcg gctcgacagg ctcgaggaga
     5281 agctcgtcga gctggatcag gcggggcggc ggcccaaatt catctacacg atcacgacct
     5341 accagaaccc gaccggcttc gtgatgccca gagccaatcg cctcgagctc atcgagctgg
     5401 cgagacgcta cgagattccg gtcatcgagg acaactgcta cggcgacgtg cactacgagg
     5461 ggccggtgga accggcgctg tatgccctgg atgacgatcc caaccagatc tacctgtgct
     5521 cgctgtcaaa gatcctggcc cccggactgc ggctgggata cctcattgcc cgaccgccga
     5581 tgctcgaacg cattctggcc aggcgtcatg acgccggcag caacacgctg gcggcagcca
     5641 tcgccgcgga gttctaccgg gacgggatat gggaccacac ccgctatgcc aacgccgcac
     5701 tcaaggaaaa gcgcgatctg ctgttcgacc gtctccaggc agagctggac gacgtctgcg
     5761 tgtggtcgcg acccgtcggc gggctcttcg tgtgggtccg gatacccgac gacatcaacc
     5821 gcaagcagct ctacgagctc gctcagagca gagggttcaa ctacctgccc ggcatcagct
     5881 ttcacgtgga gacgcgcaac gtgccctatc tgcggctagc tttcggccac ctgaccctgg
     5941 atcagatccg cgacggcatc cccgtcctgg cgcagtgcat ccgggagtgc cgcaccagca
     6001 acgagccgcg cggcttcgat tccctgtttc aggatcggga gggctcttcg gcgtagcgct
     6061 tcaggcgttc gagctggccc agctggaccc cgtccacggg ttcggccatg gattcggcac
     6121 cgaacccggt cacgtgatgc cggtactcga gccgggcgcc gtcggcggcc gcggacagac
     6181 ggaactccac ggcgccggtt gccccatacc ctggagcggg ccaagcccgc ccaacatccg
     6241 cagaaccgcg ccaggctcga cgtagaccac ctgcatgtgg gcaatgctgc cgccgccagg
     6301 cagcttctcg cagaagcacc cgagcgcgcg aggttcaaga taaaagttag ccgcgtcgcc
     6361 ggagtaagaa ttggccgcgt cccaccaggc atgcacctct tcggtgagag cgcgatacac
     6421 gcgctcgggc ggcgcatcgc cggttcaggc gctcgggcga cgcccgcgca gagcgccgcg
     6481 aatccggtcc atgaaggtgt cgtatttcag gtcggtgaac tcgccggcat cgaagaacat
     6541 gccttcgcat tgcgggcagc tttcgtacca gatgtgcgtc tgccggtcgt cggtcttctt
     6601 ctccatcttc acgccgcact cggggcagct gatgttgtcg agcttgtcca gcgcctgacc
     6661 gagcttggga tcacccgagt cgagaatgac gtccgccatc cactcgcgct tcatctcgag
     6721 aagcacctca ggcttgcacc acaagccgcc gcagtccgaa caccgatgca cagtgatttt
     6781 gcggccgtag gtcttcgtct cgagcgttcc gggacatttc ggacattcca tgaacagatc
     6841 tcctaccgat tgaatttcgt atccggcttg taataccgac tgtgccagat tccttccggt
     6901 gcttctcctg ccaccggcat tctccaggtc ttgatttcac cttcgctcat cacgacgcga
     6961 tacagcccgc ggggctgatc gatcgtattc tgcacgtaag cttccgcttc cgcatcgtct
     7021 acgtaacgct tcgcgatccg ccggtaaaga tctcgccaga catcgtcctc gccgacgtcg
     7081 tgcacaagct ccgcgcgccc ctcgacgatg actttgcgat acggcaacgg ctgctcgtcg
     7141 atgcacagcg aaacccgcgg gtcgcgccgc aggcaggcaa accattcgga tttctccctg
     7201 ggagtgaaaa atatcgcccc gtcctcgtga agaaaccaga tcggggtcac cagcggcgag
     7261 ccgtcggcac gaaccacgcc gatcctcatg acgatcccac gctcggcaag aaaatcgtcg
     7321 cgctctgcaa tagacagtgc gggcacgccc cctccccctt tagttcctag ttgccgaaat
     7381 cccaggactc gccggcatgg tatcggcgca gtatgttctt ggcgatgagg attcgatgga
     7441 cctcgtccgg tccatccgcc aggcgcaggg tgcgcgcgcc ctgatacatg ctcgccagcg
     7501 gcagatcgcc cgacacgccc gcagcgcccc agacctgaat cgcgcgatcc accacccggt
     7561 ggtaagcctg aggcacgaat atcttgatgg cggaaatgtc cgtgcgcgga tccgctccgc
     7621 tttccatctt ttcagcgcaa tggatcgtca tcagcctggc ggtctgaata tcgatgtagc
     7681 tgtcggcgac gaagccctgc atgaactgtt tgtcttccag tttctcgccg ccgtggactt
     7741 cccgcgtcat gagacggtcg atcatcaggt cgaacgcgcg ccacatggag ccgatggagt
     7801 tcatgcagtg ataaacgcgg cccgctccga gccgatcctg ggccgcctga tgcccggtac
     7861 cggtgcggcc gagctgattc tccttcggca cgcgaacgtt gtcgtagacg atctcgcagt
     7921 gactggacgc tcggccccac accggtactc cgcgcacgat gttcaccccg ggagttttgc
     7981 gcggcacgat gatctgcgtc atcttctcgt tggcgccgcc gggatcgtcc ggatccgacg
     8041 tccggcacat cacgatatag aaatcggccg caaacccgtt cgaggtgaac cacttgtgcc
     8101 cattgatgac ccattcatcg ccatcgagct ccgcccgggt cgtgatcgag cgtggatctg
     8161 atcccgggtt gtgcggctcg gtcatggaaa agcccgactg ggcggtgccg tccgtcagcg
     8221 gcttgagcca tttctcgtgc tgatccggcg tgccgtactt gaccaggatc ttctggttgc
     8281 cggaattcgg cgccacgacc ccgaacaggg aagccgcacc ggggctgtag gccagcacct
     8341 cgttcatgta cgcgtgctgc atgaaagtca ggcccatgcc gccgaattcc cgcggcaggt
     8401 gcggcgccca gagccgggcg ttcttcactt tctcctgaat gtcgacacgc agcgccttgg
     8461 cttcttgctt gacctcgttg gtcgcgttgg cgctgtcggc taaccaaccc cacaagcggt
     8521 cctcgttggg cagaatgtcc tcattgacga tctcggcggt ggccagccgg atctcgttga
     8581 cctcctcgtc gagcgtcggt accggcttca cattcatcga cacgacaatt cctccctcat
     8641 gcggccagtc gattaatcta attgaacacg gcccgcattg cgactgaccg gtacccgggt
     8701 gccggggccc gctactgcag attgggcttt ttggtatgct gctggatcga ggactgcaaa
     8761 ccgggaacga cgatgggcac tcacgagctc aactatccgc acgccgagca tccggcaccg
     8821 ggaaccacgg tcgagctcgc cccgggcgtg cgctggctga ccatgcccat gggcggctcg
     8881 ctgaaccaca tcaatctgta tctgctggaa gacacgaccg gctggtggat agtggacacc
     8941 ggcttgagcc tcgaccagac ccgggagctg tggcacgaga tcttctcccg agagctccag
     9001 ggcaggcccg tcctgggcgt catctgcacc cactttcatc cggaccacat cggccagtcg
     9061 aagatgatca ccgacgagtt ccgctgcagc ctctacatga cccgcgccga gtactaccag
     9121 gcccgcgcct tttcgggcag cgggccgacc tatcagtcca gctggctggg cgagacgttc
     9181 taccagcgcg ccggcatgcc cgcagactac ctcgagcaac tccgcgagat gtgggcgcac
     9241 cggtcttcgg aaggcatgtc gatgcccgac atgccggccg gctaccggcg cctggaggac
     9301 ggggacgttc tcaccatcgg cggtcacgac tggcgcatcg tggtgggctc gggccactcg
     9361 cctgaacatg cctgcctgta ctgcgcggcc ctgaagatca tgatctcggg cgaccagatc
     9421 ctgccagtga tcacgagcaa cgtcagcgtg caccccaccg agccagaggc gaacccgctg
     9481 aaggactgga tggaatccca cgaccgattc ctgcgggtcc ccgacgacac cctcgtcctg
     9541 ccggctcaca acctgccgtt ttacggcgtt cgggagcggc tgcgccagct gatcgaccac
     9601 cacgaggatc gcatgctggc catcgaggaa cactgcgtcg agcccgcggt ggcgaaggac
     9661 ctgcttcccg ttctattcgc acgcgagctc gacccccgtc agatgatgat ggccctgggc
     9721 gaagccatcg cccatctgca cctgctggtg catcgcaacc gcatcgagcg gacgctgcac
     9781 gaggacggct gctaccgctt cctgtccatc gacccggacc tgaaacggcg agcgcatccc
     9841 ggggaccacg atgccccgag cgagcggccc gtgctggtct gagccggaag agcggagcca
     9901 cacggcattg cagaagggca aactcaaacg gcgaattgcc gactacacct accgcatgat
     9961 gcattggggt cagaaccgcc tgcccccggg gatccgctct ctggtgggcg tgctcttaat
    10021 ggtcggcggc gttttcggtt ttctacccgt gctcggcttc tggatgctgc cgctgggcgt
    10081 cgccttcgtc gccctcgacg ttcccggcac gcgacgccac atcgacatgt ggatgctgcg
    10141 cctgtaccgc caggcctacc tggacgagcc tacgtccaac cagtaacccc cgccatccag
    10201 gcgccggacg tgaccggcgg tcggcgtcgc aaagtgggtc ccgatgatca ggacgtcgga
    10261 gtccacgtag gattccagga gccgctcccg ggtcgcttca ccagcagcac ggtcgtagtc
    10321 ggccgaggag caccagtcgg tccgcgtcat ctggcaggga tggtggatgc agtcaccggt
    10381 gatgagcgcc tccttgccgt tggatcgaat gtgcacgctg acgtgcccgg gcgtatgacc
    10441 gggcgtcggc tccagagcga cctcgggaca cagctgatgc tcccagtcca cgaagtccat
    10501 caggccggcg gccacgatgg gccgcacgga atctgccatg atcccctggt tgatctcgtc
    10561 ctcggcttcg ccgtcccagt agcggaactc cttctcggcc accaggtagc gggcgttcgg
    10621 gaacgtgggc acccactcac cgtccacgag catggtgttc cagcccacgt gatccacgtg
    10681 cagatgcgtg cacagcacgg tgtccacggt ttcgcgcgga tagcccgcag cgccgagatc
    10741 ttcgagaaag ctggtctgaa ggttggtcca ggcaggtatt gaccgctgct tgtcgttgcc
    10801 gatgcaggtg tcgacgatga tgcggcgcga gcccgtatcc acgacgagcg cgtggatgct
    10861 catgatcagg ttccccgctt cgtccatgaa gtgcgggtga agccactcga tgggcaggca
    10921 ggcacctctg gtcgcatccg gcagaatgaa gcgggtgcca ccggccacct ccatttccac
    10981 cacacgcgtg atcttcacat cacccaccat ccaatggttc atcgccgctc cttgtctgcc
    11041 cgggccgaac tgagcctggt tgagccccga gcatgccaca ggtcgcttga tggtggcata
    11101 cccgcaggat aaggtgcccc acgtagccga acgaggagaa agcatgggcc cgatcgccaa
    11161 ggccgtggac gtcgtccggg cgctgagtaa cgtgcctacg ctgcttgctc tcaagcgcgg
    11221 catgaagccg aggcccgtgg acgagcacga ctgcttcgcc gcgaacgtgg agcgcaacgc
    11281 cgccgcgttc ccggacagaa ccgccatcat cttcgaaggc aactcggtaa cctgggccga
    11341 gttcaacgct cagtgcaacc gctttgcgtc ggcattcaag gctcagggtc tccgcaaagg
    11401 cgacgcggtc agcgtgatga tggagaaccg catcgaactg ctggcgacca tcgtggcgct
    11461 caacaagctg ggcgtggtgg ccggcctgat caacaccaac ctacgcggcc gacccctggc
    11521 tcactgcatt acggtgacgg agtcgcggac gtgcgtgttt ggagaagagg tgggcgaagc
    11581 cgtcgctgaa gtgaaggacg agttgcccct ggaagagggc tccggctacc tgttcgttcc
    11641 ggatgcaggt agcggcgcgg caccaaactg ggcggccgat ctcagccgcc ttgcggccga
    11701 cgccgacgac gccgaccccc cggagacggg cgacgtgcag cttggcgacc gggctctgta
    11761 catctttacc tccggcacca ccggattgcc aaaagcggcc gtactctcca atcgccggtt
    11821 cgtcgccacc gccgcgctcg tccacaaagc cgggctgcgc tgcagcgagg aggacaggat
    11881 ctatatctgt cttcccctct accacggcac ggggctgctg atcggcgtag gcgcttcgtt
    11941 ctcgtccggc gcaagcatgt tcattcgtcg aaagttctcg gccagcaact ttctgcgcga
    12001 ggtccgcgag tatcagacca gctgcctgat ctacatcggc gagctgtgtc gctatctaat
    12061 gaacacgccc gcccagcccg acgatcacga caacccgctg gtcaccatga tgggcaacgg
    12121 cctgcggccc gacatctggc ccgcattcaa atcccgcttc gggatcaagc gcatcaccga
    12181 gttctacggc gccagcgagg gcaacgtctc gttcgtgaac ctgctgaaca aggactgcac
    12241 catcggcatg acgggatcca aggttgcgct cgtccgctac gacgtggacg aggacgagat
    12301 cgtccgggac gagaacggcc actgcatgga ggtggcgccg ggcgagcccg ggctgctgct
    12361 cggccacatc agtgaggacg ccgttttcga gggctatacc aacgcggaag cgacggaaag
    12421 caaggtcgtt cgcaacgcct tcgaggacgg cgacgcctgg ttcaactcgg gcgatctcat
    12481 ccgccaggta gacgtaggct acagcctggg ctatccccac taccagttcg tggaccgcgt
    12541 gggggacacc ttccgctgga agtcggagaa cgtctccacc aacgaggtgg gcgagctgat
    12601 caacggtttc cccgacgtga agttctgcaa cgtttacggc gtcgagattc cgggggcgga
    12661 cggccgtgcc ggcatggtcg ccatcaccct ggcggacggc gtgacagaac tcgacgcgga
    12721 cgccctgtcc cgtcacgtga gcgacacgct gcccccctac gccaggcccg tcttcgtcag
    12781 ggtgcagccg gacatcgacg tcaccggcac attcaaaatg gtcaagggcg atctgcgcaa
    12841 gcagggttac gacctggacc agatcactga tccgctgttc gtcatgaagc cgggtgaaag
    12901 tgcctacgtg cccctggacc gggaattcgc cgggaagatc gcttcaggcg cagccggcta
    12961 ctgaggcgcg ttcacgcgag ctgaggcaca accgcttcat cgagcagcgc cagtgcccgg
    13021 cgcgcctcaa ccggatcggt gccggcggtg gggccgctga tcgcgacctt ctcgatgccc
    13081 atcgacgcga gccggttcag gcgctcgacg cagtaatcgg gagggcccgc gatggcgaaa
    13141 cgatcgacga agtcgtcgtc cagcagagct gcctggctcg agtccgcccg cgtgtgcgct
    13201 ttcatgtcat agccgtcgtg caggctggtc aacgttgccc ggtcatgatc atcggcggga
    13261 ccggcaatct ggccgtgcat gacggaaaat cgtgcgaagg tcgtgagccc gccccggacc
    13321 aggtcccggg caaccgcgcg gtcacgatgg caaaccagat tcacgtacgc gccgaatgcc
    13381 ggatcatcgg gaatgccggc gcccgttctg gccgccatgg ccgtttccat tgcccagctc
    13441 agtcgctcct ccaccgcgcc aagggtgaac atcacacggt cggcgagccg ggcagccatc
    13501 tcgatcacct tgggccccgt ggccgcaact tccaccggca ccttggcgac gtcatccttg
    13561 aaccaccgga tgcggctggc gctcgggtga tcggcaagat cgagatcggc aaccggagcg
    13621 gcgatgtggt cgtccatggg aatgtcttcg aacggcacgg cttcgccctt caggtagcgc
    13681 tgcagcacgt ccagataacg ttcgaacccg ggcagtctag ccggcgatct gcccagatgc
    13741 gccaacgcgg agtccccgcg cccgatgccc agaaccatgc gcccgccgga gacgtcctga
    13801 acggccatgg cggcagaggc ggtcacggcg ggatgtcgcg tgaccgtgtt cgtgactccg
    13861 gttcccaggc cgatccgctg tgtcccggtg gctgccacgg ccaggcccac gtaggagtcg
    13921 gccgccaggt tctgcgagtc caccaccatc acgccggccc aaccggccgc ctctgcgcgg
    13981 cgggcgacgg ccagcacgtt gtccgcacgg gagtgagtca gggtccacag ttccattggc
    14041 ggggcatcca tcgtcgatgg ttctccacca tgatggttca acccctggaa aaagggaaac
    14101 cccgggctgg cgcccggggc cgtccgccta cagccagggg gaagggaggg agcatgggcc
    14161 cgctgggggc atgccgtagc gaactgggca ccggccgaat accatgcggg tcctgacgac
    14221 tgcgcacctc cgtgccggac ctgccaggca acgaccgaga ccttgaggca gaccttagag
    14281 ccgattcgct gaacgccaac tgaatgcaga aagacccgaa aatacggcgg cgcccaccac
    14341 cggcgaccat ccgggccggg cgatgaagcg cgccctgctg ctggatctgg gcaacgtcgt
    14401 cctgcgcgtg gactttcggc gtaccttcga ctactgggcc gaagcggccc gcgtgcccgc
    14461 tgagcgcctt taccgccgct ggcagctgga tgaggcatac gagctgcacg aggtgggagc
    14521 cctc
//
LOCUS       DBNHQR010000232         1588 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1732293, whole genome shotgun sequence.
ACCESSION   DBNHQR010000232 DBNHQR010000000
VERSION     DBNHQR010000232.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1732293"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1588
                     /locus_tag="AC16VR_05400"
     CDS             <1..>1588
                     /locus_tag="AC16VR_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012076390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HIR6010626.1"
                     /translation="WGAHEGSFLLWTLIMSGWTLAVAVLGRTLPLEFHSRVLGVLGLI
                     SVGFLVFIIYTSNPFGRLLPPAAEGMDLNPLLQDPGLIIHPPMLYMGYVGFAVAFAFA
                     VAALLGGQVNREWVRWSRPWTHVAWAFLTAGIALGSWWAYYELGWGGWWFWDPVENAS
                     FMPWLVGTALIHSLAVTEKRGTFKSWTVLLAITTFSLSLLGAFIVRSGVLTSVHAFAV
                     DPERGLFILGFLILIVGGSLLLYGVRAGVVASRARFEPLSREAFILLNNVLLVVSMAV
                     VLLGTLYPLAYEAVSGGDKISVGVPYFNAGFVPLMLLLAAALATAPLLRWKRTERREI
                     LRGLLPALGVSLLLGVGAPLVLAGGVSWQVALSVALACAILATHSRDLWTRARRGVAR
                     VPLAYWGMFAAHAGFAVAILGIALTSQWSVEKDLRMSPGEVVVLDGRAIEFLGVNPHR
                     GSNYVADRGSFSVQEGDARIVLQPEKRRYLAGGNVMTEAAIDAGFTRDLYISLGEPLQ
                     GGDWAVRIQVKPFVRWIWLGG"
BASE COUNT          189 a          516 c          545 g          338 t
ORIGIN      
        1 ctggggtgct cacgaagggt cttttcttct ctggacgctg atcatgtccg gctggacgct
       61 tgccgtggcc gtgctgggac ggaccctgcc gctggagttt cacagccggg tgctgggcgt
      121 gctgggcctg atctcggtgg gcttcctggt cttcatcatc tacacctcca accccttcgg
      181 acgactgctg cccccggccg ccgagggcat ggacctcaac cccctgctgc aggaccccgg
      241 gctgatcatc catccgccga tgctgtacat gggctacgtg ggctttgcgg tggccttcgc
      301 gtttgccgtc gccgccctgc tgggcggaca ggtcaaccgc gaatgggtgc gctggtcgcg
      361 tccctggacg catgtcgcgt gggcgttcct gaccgccggc atcgcgctgg gcagctggtg
      421 ggcctactac gagctcggct ggggtggctg gtggttctgg gacccagtgg agaacgcatc
      481 cttcatgccc tggctggtgg gcacggcgct gattcactcc cttgccgtga ccgagaaacg
      541 cggaacgttc aaaagctgga cggtgctcct ggccatcact accttttcgc tgagcctgct
      601 gggcgcgttc atcgtgcggt ccggtgttct cacctcggtt cacgccttcg cggtggatcc
      661 tgagagaggg ctgttcattc tgggcttcct catcctcatc gtgggcggct ccctgctgct
      721 ctacggcgtg cgggccggcg tcgtcgcgtc ccgggcccgt ttcgagccgc tcagccggga
      781 agccttcatt ctgctcaaca acgtgctgct ggtggtcagc atggcggtgg ttctgctggg
      841 cacgctctac ccgctggcct acgaggcggt gtcgggcgga gacaagattt ccgtcggcgt
      901 gccgtatttc aatgcgggat tcgtccccct catgcttctg ctcgcagcgg cgctggctac
      961 cgcgcccctg ctgcgctgga agcgcacgga gcgacgcgag attctgcgcg ggctgttgcc
     1021 cgcgctcggg gtgagcctgc tgctcggtgt gggcgcgccg ctggtgcttg ccggaggggt
     1081 atcctggcag gtcgcccttt ccgtggcgtt ggcctgcgcg atcctggcca cgcactcacg
     1141 agacctctgg acccgggcgc ggcgcggcgt cgcccgcgtg ccgttggcct attggggcat
     1201 gttcgccgcg cacgccggct tcgcagtggc catcctgggc atcgcgctga cctcgcagtg
     1261 gtccgtggaa aaggacctgc gcatgtcccc gggcgaggtc gtcgtgctcg atggtcgggc
     1321 catcgagttt ctcggcgtca atccccatcg tggttccaac tacgtggccg accggggcag
     1381 cttcagcgtg caggagggcg atgcccgcat cgtcctgcag cccgagaagc gtcgctacct
     1441 ggcgggcggc aacgtcatga ccgaggccgc catcgatgct gggttcacgc gcgaccttta
     1501 catctccctg ggcgaacccc ttcaaggagg tgattgggcc gttcgcattc aggtgaagcc
     1561 gttcgtgcgc tggatctggc tgggcggg
//
LOCUS       DBNHQR010000233         3978 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_909036, whole genome shotgun sequence.
ACCESSION   DBNHQR010000233 DBNHQR010000000
VERSION     DBNHQR010000233.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_909036"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            232..834
                     /locus_tag="AC16VR_05405"
     CDS             232..834
                     /locus_tag="AC16VR_05405"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR6010627.1"
                     /translation="MASILFLRHGQIAANAKGRWNGSTDSELTWRGRRQARRTAKHLA
                     AREAEITAIYSSPLRRTRFTAEAAADRLGLPVTIADGLREYAIGEWEDLPFRHLAREH
                     DFVNTTIRDPDFAPPGGESLRDVADRVVPTILEIHQRHREDSKVLVVGHGAAMAVAFG
                     AILERNPARWTNYHFANCSLSELVISPAPYVNFFNSTQHL"
     gene            862..1632
                     /locus_tag="AC16VR_05410"
     CDS             862..1632
                     /locus_tag="AC16VR_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008370376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6010628.1"
                     /translation="MYEYCSVEKDDHIMTVTIERPDRLNALHPPANAELGEVFDDFAA
                     NDDMWVAIITGRGRGFSAGNDLRYQAEGGERVPMPRGFGGLTSRFDLNKPVIAAVNGV
                     AMGGGFEIALACDLIIASEKALFALPEPKVGLAALAGGLNRLPRQIGPKRALGMILTG
                     RHVPAEEGLQLGFVNEVVPHDELLAATRRWAEQICACAPLSIRASKDVVYRSLDSASL
                     QASMQAQYDSVKAMVKSEDFIEGPKAFAEKRPPAWKGR"
     gene            complement(1658..1855)
                     /locus_tag="AC16VR_05415"
     CDS             complement(1658..1855)
                     /locus_tag="AC16VR_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010629.1"
                     /translation="MKDPAVRSYPEQFATTPDLLHVVIPRIEIIAEMAVRDERDALND
                     VCLAVACVADAIGSAQLAAQG"
     gene            complement(2105..2836)
                     /locus_tag="AC16VR_05420"
     CDS             complement(2105..2836)
                     /locus_tag="AC16VR_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4336 domain-containing protein"
                     /protein_id="HIR6010630.1"
                     /translation="MRLFDRALSRLHPEQLPVMGARLRSIGRQIWVADRELQMPGGIR
                     LPIRMVVMGDGRGNLLCYSPVELDEGTAEALDGLGKVRWIVAPNRYHRSFTAGCMSRY
                     PQAQVSGFLPPPGDGFAAPAAVPFGGCELTTVSLRTGFAEIVAYHDLSETLVVADLLF
                     NVRNGNRRLQAVMRVNGAWQKAGHTRLQRWLILRDPDGLTKFRRWALSRPFVQISMAH
                     GLIVQEDARECLYRLQRGGPFGAPH"
     gene            complement(2862..3647)
                     /locus_tag="AC16VR_05425"
     CDS             complement(2862..3647)
                     /locus_tag="AC16VR_05425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR6010631.1"
                     /translation="MGPQSSVETMTRCAVGAERLGFESLWITDHIAIPPDDAEGSGGR
                     YLDTLTTLAYLGGVTRTIRLGSGVIILPYRPPLPTAKQVATVQELTGGRLLLGVGIGW
                     MDAEFRALGVDRHQRGRISDETLDYLNRCFTEEVMEANGQPFLFRPCPDKPPVYVGGS
                     APHALLRAARYGDGWLPMALEPDPLGEAMVEYRRITEELDRPAGHVTLMRGLPLGKPD
                     ATRALLEQYQGLGLDRLVCALGYDDADAYLHQLEQLAELIDSK"
     gene            3784..>3978
                     /locus_tag="AC16VR_05430"
     CDS             3784..>3978
                     /locus_tag="AC16VR_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HIR6010632.1"
                     /translation="MRSLCLMPQAIYLLAFVGGFVIMSLELLGGRVLAPYFGSSIYVW
                     GSIITVFMLSLSLGYLWGGRL"
BASE COUNT          745 a         1334 c         1228 g          671 t
ORIGIN      
        1 catggcgctg ataaccgggg tcgccacatc ccgccaggag accgaccagc gtccagacca
       61 gccaccgctt ggcgccatca cgtatcaatc gcggcgcgct gcgcacaatc acaccctcga
      121 agtagacaag cctgcacacc aggaaatacc atcccgcccg catcgcgccc gacaccgggc
      181 ttcacaccgc gcgcccctgt ggggcgcccg gcatcatgac gtaaatcgaa catggcctcg
      241 atcctctttc tacgccacgg ccagatagcc gccaacgcga agggccgctg gaacggctcc
      301 acggacagcg agctgacgtg gcgcgggcgc cggcaggcac gccgcaccgc aaagcacctg
      361 gccgcgcggg aagcagagat cactgccatt tactccagcc cgctgcgccg gacgcgattc
      421 acggccgagg cagccgccga caggctcggt ctgccggtaa ccatcgccga cgggttgcgg
      481 gaatacgcca tcggcgagtg ggaggatctc ccttttcggc acctcgcccg cgagcacgat
      541 ttcgtcaaca cgaccatccg cgatcccgac ttcgcgccgc ccggcggtga atcgttgcgg
      601 gacgtagccg accgcgtcgt gcccaccatt ctggaaatcc accaacgcca ccgggaagat
      661 agcaaagttc tcgtcgtcgg ccacggcgcg gccatggccg ttgcctttgg agcgatactc
      721 gaacgcaacc ccgcgcgctg gaccaactac cactttgcca actgcagcct gagcgagctg
      781 gtgatttcgc ccgcgcctta cgttaacttc ttcaactcga cccagcacct ctgaccgacc
      841 tcaacgggag aacgaaagcg catgtacgag tactgcagtg tggaaaaaga cgatcacatc
      901 atgacggtca ccatcgagcg acccgaccgt ctcaacgcgc tgcacccgcc ggccaacgcc
      961 gagctcggcg aggtattcga tgatttcgcc gctaacgacg acatgtgggt ggcgatcatc
     1021 accgggcgcg gcagagggtt cagtgcgggg aacgacctgc gctaccaggc cgaaggtgga
     1081 gagcgcgtac ccatgccccg gggattcggg ggcttgacca gccgtttcga tctcaacaaa
     1141 ccggtgatcg ccgcggtcaa cggcgtggcc atgggcggcg ggttcgagat cgcgctggcc
     1201 tgcgacctga tcatcgcctc tgaaaaagcc ctctttgcac tgcccgaacc caaggtcggt
     1261 cttgctgcgc ttgccggcgg gttgaaccgg ttgccgcggc agatcggccc gaagcgcgcg
     1321 ctcggcatga ttctcaccgg ccgtcacgtg cctgccgagg aaggattgca gctcggcttc
     1381 gtcaacgagg tggttccaca cgatgagctg ctggccgcca cgcgccggtg ggcggagcag
     1441 atctgcgcct gcgccccctt gtcgatccga gccagcaagg acgtggtcta tcgcagcctc
     1501 gactccgctt cgctgcaggc gtccatgcag gcccagtacg acagcgtcaa ggccatggtc
     1561 aaaagcgagg atttcatcga gggtccgaaa gcgttcgcgg agaagcggcc gccggcgtgg
     1621 aaggggcgct aaagccagga aaattccagc ggcggcgcta tccctgcgcc gccaactggg
     1681 cggagccgat ggcatccgcg acgcaagcta cagccaggca gacatcgttg agcgcatcgc
     1741 gctcgtcccg cactgccatc tcggcgatga tctctattct tggaatgacc acgtgcagca
     1801 ggtccggggt ggtcgcaaac tgctcggggt atgacctgac tgccggatct ttcatgctct
     1861 gcctctcgtc catctcccgc taagcggccg tcagcacatg gcattcaggt ttcgtgccag
     1921 tctgaaatac cttgataagc cattgtttta tatattttta ttcccatata gcgccacgat
     1981 tcgaaacgtg ccgaaggtga cgcttgggag aagaaactga ccgggccggg cccggacgga
     2041 gccaaccggc cgctattgcc tgcagagtcc tggccggccg ccgaccccac acgcgatcag
     2101 cgtttcagtg gggcgctccg aacgggcctc cccgctgcag ccggtaaaga cactcccgcg
     2161 catcctcctg cacgatgaga ccgtgagcca tggagatctg cacgaagggc ctggacagcg
     2221 cccaccgacg gaacttcgtc aggccgtcgg gatcgcgcag aatcagccac cgctgaagcc
     2281 tggtgtgtcc cgctttctgc cacgctccgt tgacgcgcat gaccgcctgc aaccgccgat
     2341 tgccgttgcg cacgttgaac agcagatcag caaccacgag ggtttcggag aggtcgtgat
     2401 aggcgacgat ctccgcgaag cccgtacgca gggacaccgt ggtcagctcg catcctccga
     2461 aggggacggc ggccggggcg gcgaacccgt cgccgggggg cggcaggaaa cccgacacct
     2521 gagcttgcgg gtagcgggac atgcacccgg cagtgaagga acgatggtag cggtttggcg
     2581 caacgatcca gcgcaccttg ccaaggccat ccagtgcctc ggccgtccct tcatccagct
     2641 ccaccgggga atagcagagc agattgcctc tgccgtcacc catcaccacc atccgaatgg
     2701 ggagcctgat tccgcccggc atctgcagct cgcgatcggc cacccagatc tgcctcccga
     2761 tggaccgaag ccgcgcgccc atcaccggga gctgctccgg gtgcaggcgg ctcagcgccc
     2821 ggtcgaagag gcgcacgaac atctgcccga tcaaagtgct ttcacttgga atcgatcagc
     2881 tcggcaagct gctccagctg gtggaggtac gcgtccgcat cgtcatagcc aagggcacag
     2941 acgagccggt ccagcccgag gccctggtac tgctcgagca gcgcccgggt cgcatccggt
     3001 tttccaaggg gaagccctcg catcagggtg acgtgcccgg ccggccggtc gagctcctcc
     3061 gtgatgcggc ggtattccac catcgcctcg cccagcggat caggctcgag agccatcggc
     3121 agccatccgt cgccgtagcg tgccgcgcgt aacagcgcgt ggggagccga cccgcccacg
     3181 tagacgggag gtttgtcggg acacggccgg aagaggaagg gttggccgtt ggcctccatg
     3241 acctcttcgg tgaagcaacg attcaggtag tcgagcgtct cgtcggagat gcggccgcgc
     3301 tgatgcctgt ccacccccag agccctgaac tccgcgtcca tccagccgat acccaccccg
     3361 agcagcagcc ggccaccggt tagttcctgc accgtggcga cctgcttggc cgtgggcaga
     3421 ggcggtcggt agggaaggat gatgacgccg gacccgagac ggatggtacg ggttacgcca
     3481 cccaggtagg caagagtggt cagggtgtcc aaatagcggc cgccgctccc ctcggcgtca
     3541 tccggtggga tggcaatgtg atcggtgatc caaagcgact cgaatccgag ccgttcggct
     3601 cctacggcgc accgcgtcat tgtctcaacc gaagattgcg ggcccatgtt ccgcaacgtc
     3661 acaccgatct gcacgggcag ccctcctttg cgattgccga agacaagctt tgcgcgtcag
     3721 gcccgagtct tcaaccggtg cgagttgtgg cgggacggga actttgtaaa ctgcaccctc
     3781 ccattgcgca gcctgtgcct gatgccccaa gccatctacc tgctcgcctt cgtcggcggc
     3841 ttcgtgatca tgtccctgga actgctgggc gggcgggtgc tcgcgcccta cttcggcagc
     3901 agcatctacg tctggggcag catcatcacc gtattcatgc tgtctctgtc gctcggctat
     3961 ctttggggag gccggttg
//
LOCUS       DBNHQR010000234         3143 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_23220, whole genome shotgun sequence.
ACCESSION   DBNHQR010000234 DBNHQR010000000
VERSION     DBNHQR010000234.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_23220"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1001
                     /gene="xrtE"
                     /locus_tag="AC16VR_05435"
     CDS             <1..1001
                     /gene="xrtE"
                     /locus_tag="AC16VR_05435"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exosortase E/protease, VPEID-CTERM system"
                     /protein_id="HIR6010633.1"
                     /translation="ILGTDTFRVSIDTACSGYEGIGLVVVFTAFVLSLFRADFRFPQA
                     LLLFPIGIAAIWSFNAIRIALLIGIGHELSPEIAVGGFHSQAGWISFVLVAVGLLAGA
                     YRISFFRADTPVPTRSAVVTAGLNANVAYLLPIVALLGSTLLTLAFTADMDWLYPLRV
                     VMVGAVLALAWRHFRFVWAPVSWVAVGGGFLVLALWLALVPTDPAQDALVGAKIGEAP
                     LWIAALWIIFRCVGSAVTVPLAEELAFRGYLMARLTPGEPSPADRLPFSWLALLVSSA
                     VFGLLHGAWLAGAIAGLTYGLIRYHRGRVADAVVAHAVTNALLSVYVLVTGHWSLW"
     gene            complement(1055..1858)
                     /gene="hpaH"
                     /locus_tag="AC16VR_05440"
     CDS             complement(1055..1858)
                     /gene="hpaH"
                     /locus_tag="AC16VR_05440"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003209880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HIR6010634.1"
                     /translation="MLNPEQARAAAMELDTAERGRRQIRPTSVTFQGMTVEDAYAVQD
                     AWVKLKIDSGRRIVGRKVGLTSRAMQEAMQIAEPDFGTLLDDMMFEDGASLKAGDFLD
                     PRIEVELAFVLGQTLAGADVTLDDVLEATDYVTPALEIIAARSFRVDPQTGSPRTVVD
                     TISDNAANAGVIVGGRRVRPDELDLRWVSALLYRNGIVEETGVAAGVLDHPGNGVVWL
                     ARKFAEHGVSLERGEIVLAGSFTRPVAVRPGDEFRADYGPLGVIGCAFS"
     gene            complement(1872..>3143)
                     /locus_tag="AC16VR_05445"
     CDS             complement(1872..>3143)
                     /locus_tag="AC16VR_05445"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6010635.1"
                     /translation="VLTVVGIDPRARVSDNGASRENLAMDHLFDGLKVVDAASFLAGP
                     GAGTVLSDFGADVIKVEPLHGDGYRTLRGNYPIDYNWQLTSRNKRSIALDLDQEEGRE
                     VLRTLMADADVLLVNYIGDQLHRFGLAYETVRQHNPGIVYAHLTGYGTEGPDAAKRGF
                     DSTAWWARTGMMDLVRDPGQAPLIGVPGFGDHSSAMSLFGAIMMGLYRRERTGEGSYV
                     STSLVANGVWANGMQLQGVIAGIDLGAIRQEKGWTNPFTSVYGSSDGRYVLLTVTNQA
                     REWPALCKALGHQEWLEDERFADFRTLFRNRVELIRLIGEATQTMTLSELTEALDAHG
                     ITYGVVSQMAEVVDDPHLRAAGIIVDTASPDESYAATIANPISMAEAPKRSHGRAPEI
                     GEHSREILREHGFTDEQIDELERCRVIGQHR"
BASE COUNT          485 a         1099 c          981 g          578 t
ORIGIN      
        1 gcatactcgg caccgatacc ttccgcgttt ccatcgacac cgcctgctca ggctacgaag
       61 gcatcgggct ggtcgtggtg ttcacggcct tcgtcctatc cctgttcagg gccgacttcc
      121 gcttccccca ggcgctgctg ctgtttccca tcggcatcgc cgcgatctgg agcttcaacg
      181 ccatccgcat cgcgctgctc atcggcatcg gccacgagct ctcacccgag atcgcggtgg
      241 gaggcttcca cagccaggcc ggctggataa gcttcgtcct ggtcgccgtg gggctgcttg
      301 ccggtgccta ccgcatttcg ttcttccgcg ccgacactcc cgtacccacg cgatcggccg
      361 tggtcacggc gggcctgaac gcgaacgtcg cctacctgct gcccatcgtc gcgctgttgg
      421 gatcgacgct tctgacgctc gccttcaccg ccgatatgga ctggctgtac ccgctgcggg
      481 tcgtcatggt cggcgccgtc ctggcattgg cgtggcgcca ctttcgattc gtctgggcgc
      541 ccgtgtcctg ggtcgccgtg ggcgggggat ttctcgtatt agcgctgtgg ctggcgctcg
      601 tgcccacgga tcccgcccag gacgctctcg tcggcgcaaa gatcggcgaa gccccgctct
      661 ggatagctgc gctgtggatc atattccgct gcgtcggctc ggcggtcacg gttccgctcg
      721 cggaggagct ggcctttcgc ggctatctga tggcccggct cacgcccgga gaaccatccc
      781 ccgccgaccg cttacccttc tcctggctgg cgctgctcgt ctcgtcggcc gtttttggcc
      841 tgctgcacgg ggcctggctg gccggcgcca ttgccgggct cacctacggt ctgatccgct
      901 atcaccgcgg ccgcgtagcc gatgcggtgg tcgcccacgc ggtcaccaac gcactgctgt
      961 cggtctacgt gctcgtcacc gggcactggt cactctggta gcggccggcg gtcgagcccc
     1021 cgacaaagcc acaacaagcc acacaccggc cctatcagga gaaggcgcag ccgatgacgc
     1081 ccagcggccc gtagtcagcc cggaactcgt cgccgggtcg aacggccacc ggccgggtga
     1141 aggaccccgc cagaacgatc tccccccgct cgagggacac gccgtgctcg gcgaactttc
     1201 gggccagcca gaccacgccg ttgcccggat gatccagcac cccggcagcc acgccggtct
     1261 cctcgacgat tccgttgcgg tacagcaagg cagacaccca gcgcagatcg agctcatcgg
     1321 gcctcacacg gcgtccgccc acgattacgc ccgcgttggc cgcgttgtca gagatggtat
     1381 cgaccaccgt ccgcggcgaa ccggtctgcg ggtccactcg aaagctgcgg gccgcgatga
     1441 tctccagcgc cggggtcacg tagtcggtcg cctcgagcac gtcgtccagc gtgacgtccg
     1501 caccagccag ggtctgtccg agcacgaaag ccagctccac ctcgatgcgc ggatccagaa
     1561 agtcgcccgc cttcaggctc gcgccgtcct cgaacatcat gtcgtccagc agcgtgccga
     1621 aatcgggctc tgcaatctgc atcgcctcct gcatggcccg ggacgtgagc ccgactttgc
     1681 gcccgacgat gcgtcggccg gaatcgatct tcagcttgac ccacgcgtcc tgcaccgcgt
     1741 aggcgtcttc tacggtcatt ccctgaaacg taactgaagt cggccggatc tgacgccggc
     1801 cgcgctcggc ggtgtcaagt tccatggccg ccgcgcgagc ctgctcgggg ttcagcatgg
     1861 gcgcggtgtc actacctgtg ctggccgatg acgcggcatc gctccagctc gtcgatctgc
     1921 tcgtccgtaa agccgtgctc gcggagaatc tcacgggagt gctcgccgat ctcgggcgcc
     1981 cgcccgtgag agcgtttggg ggcctcggcc atgctgatgg ggttcgcgat ggtagcggcg
     2041 tagctctcgt cgggagaagc ggtgtcgacg atgatgcccg cggcgcgaag atgagggtcg
     2101 tccaccacct cggccatctg gctgaccacg ccgtaggtga tgccatgggc atccagggct
     2161 tcggtgagct cgctgagcgt catggtctgc gtggcctcgc cgatgagccg gatgagctcg
     2221 acccgattcc ggaacagcgt tctgaaatcg gcgaaccgct cgtcttcgag ccactcctgg
     2281 tgaccgagcg ctttgcacag cgccggccac tcgcgggcct ggttggtcac ggtcaggagc
     2341 acgtaacgac catcgctgga cccgtagacg gaggtgaacg ggttggtcca tcccttctcc
     2401 tggcggatgg cgccgagatc gattcccgcg atgacgccct gcagctgcat gccgttagcc
     2461 cagaccccgt tcgccaccag cgaggtggag acatagctgc cctcccccgt tcgctcacga
     2521 cggtacaagc ccatcatgat ggctccgaac aacgacatgg cgctggagtg gtcgccaaag
     2581 ccgggcacgc cgatcagcgg cgcctgcccc ggatccctca ccaggtccat catccccgtg
     2641 cgcgcccacc aagcggtgga atcgaacccg cgcttggcgg catccgggcc ctcggtcccg
     2701 taacccgtga ggtgggcgta gacgattccg ggattgtgct gcctgaccgt ttcgtacgcc
     2761 aggccgaaac ggtgcagctg atcgccgatg tagttgacga gcagaacgtc cgcatcggcc
     2821 atgagagtgc ggagcacttc ccgtccttct tcctggtcga ggtcgagggc aatgcttcgc
     2881 ttgttgcggg aagtgagctg ccagttgtag tcgatggggt agttgcccct gagggtgcgg
     2941 tagccgtcgc catggagcgg ttcgaccttg atcacgtccg caccgaaatc cgatagcacg
     3001 gtccccgccc ccggcccggc cagaaaagag gcggcatcca cgaccttcag gccgtcaaac
     3061 agatgatcca tagccagatt ctccctgctc gccccattgt cagagacccg cgcccgggga
     3121 tcaatgccga ccaccgtcag aac
//
LOCUS       DBNHQR010000235         1838 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_593008, whole genome shotgun sequence.
ACCESSION   DBNHQR010000235 DBNHQR010000000
VERSION     DBNHQR010000235.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_593008"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1820)
                     /locus_tag="AC16VR_05450"
     CDS             complement(<1..1820)
                     /locus_tag="AC16VR_05450"
                     /inference="COORDINATES: protein motif:HMM:NF046196.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 95 catalytic
                     domain-containing protein"
                     /protein_id="HIR6010636.1"
                     /translation="MDHDCLVIMLDDRRSDPQDTCIELSMIRPARVKTEDHVAESFLE
                     LGKKRMVLKQVFTEPSVYAPLGEDHYCASALAVSVDRAGATVSMIEPGTARIRVPGQQ
                     GRGVILIASAASMDQGEDVVAAASAQLDAVAVTGVDALRSAQQAWWREFWSRSYIVTP
                     GQPEFAERWVYYLYLSACTMRGKYPAKFNGQIWVTAGDERRWGGRYWWWNQGTMHSSF
                     FAANHVELNDPLFDMRSAALPAYEKAARQDWGSQGIFIPETGTFNGPEELPEEIAAEI
                     RQVFIARQAPSERLRAICDHRHAFESRWSWHDNPARLVGWVSHVMVAGPKTAELFWQR
                     YQYTGDRDWLAERAYPMLKGAAEFYAHYPHLCPGEDGYLHLDNTSLHEHLWGCRDCVD
                     DLALMRGVLKTAAHAARLLDRDEDLQERWTEIANQLAPYPTNQTPDALGVLDSDRVTL
                     AQGVGPAALIRAWHGTESPRLRMAEQFDLLTLESADDELWQLVQNTFEAHPGTAAYLA
                     GKPGNETGRYPVIAARLGRSDVIARVLPLWLERWHGEQDFPNRFLFSGDDISVQGYGI
                     FSDALQQALCQSLAPGPGAEPVIRIFPAWPRDWDARFKLL"
BASE COUNT          336 a          586 c          595 g          321 t
ORIGIN      
        1 cagagcagtt tgaaccgggc gtcccagtca cgcggccagg ccggaaagat gcggatcacc
       61 ggttcagctc cgggtcctgg tgcgaggctc tggcagagcg cctgctgcag ggcgtcgcta
      121 aagataccat acccctgcac ggagatgtcg tccccgctaa agagaaatct gttgggaaaa
      181 tcctgctcgc cgtgccagcg ctccagccac aacggcagga cgcgggcgat gacgtctgat
      241 cgcccgagcc gggcggcgat caccggatag cggccggttt cattgccggg tttgccggca
      301 aggtaggcgg cggtgccggg atgggcctcg aatgtattct gcacgagctg ccagagttca
      361 tcatccgcgg attcgagggt gagaagatca aactgctccg ccatgcgcag ccgcgggctt
      421 tccgtgccat gccaggcgcg aatcaatgcg gcggggccga caccctgggc caacgttacc
      481 cggtcgctgt ccaggacacc caacgcgtcc ggggtttgat tggtcggata cggcgcgagt
      541 tgattggcga tctctgtcca acgctcctgg agatcctcgt cgcggtccag cagtcttgcg
      601 gcatgggcgg cggtcttcag cacccccctc atcagggcca ggtcatcgac gcagtcccga
      661 caaccccaga ggtgctcgtg aagcgaggtg ttgtccaggt ggaggtagcc gtcctcgcca
      721 gggcacagat ggggatagtg cgcgtagaat tcggcggcgc ccttgagcat ggggtaggcc
      781 cgttccgcga gccagtcccg gtccccggtg tactgatacc gttgccagaa aagttcagcc
      841 gtcttgggtc cggccaccat gacatggctg acccagccca cgagcctggc cgggttatca
      901 tgccaggacc agcggctttc gaacgcgtgg cggtggtcgc agatggcgcg caaacgctcc
      961 gagggcgctt gcctggcaat gaagacctga cggatctcgg ccgcgatctc ctcgggcagt
     1021 tcctcggggc cgttgaaggt gccggtttcg ggaatgaaga tcccctgact gccccagtcc
     1081 tgccgggccg ccttctcata agccggcaag gcggcgctgc gcatatcgaa gagcgggtcg
     1141 ttgagttcga catggttggc ggcgaagaaa gaggaatgca tggtgccctg gttccaccac
     1201 cagtaacgcc cgccccagcg gcgttcatcg cccgcggtaa cccagatctg gccgttgaac
     1261 ttcgccgggt atttcccgcg catggtacag gcgctcaaat acaggtagta gacccagcgt
     1321 tcagcaaact ccggctggcc gggagtcacg atataggacc gggaccagaa ctcccgccac
     1381 cacgcctgct gggcgctgcg cagggcatcc acgccggtca cggcaacggc gtccaactgg
     1441 gcagaggcag ccgcaaccac atcttccccc tggtccatgc tggcggcgct ggcgatcaga
     1501 atcacaccgc ggccctgctg accgggaacc cggatccggg ccgtgcccgg ttcgatcatg
     1561 ctcaccgtcg ccccagcacg gtccacgctg accgccaggg ccgaggcgca gtaatgatcc
     1621 tcacccagcg gagcgtagac ggatggttcc gtaaaaacct gcttgagtac catccgcttc
     1681 ttgcccagct cgaggaacga ctcggcaacg tggtcctcgg tcttcacccg cgcgggccgg
     1741 atcatgctca gttcgatgca ggtgtcctgt gggtcgctgc ggcgatcatc gagcatgata
     1801 accaggcagt catggtccat gctggcgaag gcgcaaag
//
LOCUS       DBNHQR010000236        10167 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_909217, whole genome shotgun sequence.
ACCESSION   DBNHQR010000236 DBNHQR010000000
VERSION     DBNHQR010000236.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10167)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10167)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10167
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_909217"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2248
                     /gene="mrcB"
                     /locus_tag="AC16VR_05455"
     CDS             <1..2248
                     /gene="mrcB"
                     /locus_tag="AC16VR_05455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 1B"
                     /protein_id="HIR6010637.1"
                     /translation="RKALGLLGLAVVLALAALTAYLVYLDRTITTTFEGRRWTEPAVI
                     YARPLELHPGAALTTAEVIAELDRLGYALQPSPPKPGAYHRSGDELDVYLRAFDFPNG
                     SRDAQRITLKFSAGSLARVQDALGRPVPLIRLDPVTIGSFFPSHGEDRLVLPPERVPD
                     LLRETLKVVEDRNFDRHAGFDLRAIARAFWVNVTAGEVRQGGSTLTQQLVKSYFLDNR
                     RTIGRKLRELAMAIILDARFGKEDLLNAYVNEIYLGQDGRRAVHGFGLGAQFYFNRPL
                     TELDIQQLALLIAVIRGPSYYDPFRHPERARARRDLVLDKMLQGGLVDEDQHARAVRS
                     PLTTVAGTRRGGAYYPAFLDLVRANLTALDRDALTTRGLAVFTTLEPQTQDDLEQAVA
                     QALGDLESSRGLEPGSLQAAAVMTATQTGEVLALSGARSAGSDGFNRALNARRPVGSL
                     LKPVVYLAALEQGLHLASLVDDAPVVLPLAGGTTWQPENFDHVTHGEVPMIRALAESL
                     NLATVNLGLEVGVGTVAARFETLIGRPPANTYPSLLLGAEAMTPVEVSALYGIFASGG
                     FYQPPKAVISVLDESRSPTLRRPLKLEQRIDTKTARALDRALQVVMARGTGRSSRFAR
                     VGVAGKTGTSDDYRDSWFAGYDDAALTVVWVGRDDNAPTGMTGASGALKVWDRIMADR
                     DVIAIPPDPDSRKVDVEFATGLVANPLCADVVTIPVPEGAALQEKPGCGIKPPSLGER
                     IRNWLRND"
     gene            2272..2733
                     /locus_tag="AC16VR_05460"
     CDS             2272..2733
                     /locus_tag="AC16VR_05460"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6010638.1"
                     /translation="MTAGCATSSVLPPVVDTNGIEDAEPAPQQEVPIPPASTPEARQP
                     DTAGATLALLEQSERAASAGDADAAVAYVERAIRLNPRSADLWLQLARLQLANEDPEA
                     AVQFANKAISLAGDRLDWVRDAWLLIADARAAQGDGDGAREIRERWRGYRG"
     gene            complement(2750..3901)
                     /locus_tag="AC16VR_05465"
     CDS             complement(2750..3901)
                     /locus_tag="AC16VR_05465"
                     /inference="COORDINATES: protein motif:HMM:NF019640.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6010639.1"
                     /translation="MRFESRRESFAELESARGLVPGIRTSQCEIDKQQALPADLAARL
                     AADGMFRLLLPVCIGGSGLSLPRFVRVVEVIAEADGSTGWCVSQAAVFASVADLLHAE
                     TAAEIWGTNRDAAVATGIPFGAEARAVDGGYLLDGRWRFASGCTHADWFAPLASVVGD
                     VGSEPSRTFLVPREEVRLGEGWNVRGMQGTGSREYRLHALHVPSRRSVADAYTSHPGA
                     STGLHVMLLFACGFAAVGLGVARRAVDEFIALAAGKTPVFANRKLAEDDLVQSGLGEA
                     EALLGATRAFLLDAAEECEHRHATTGAVDFELRSRMRLAATHAMRRSADVVDRVYAMA
                     GTDCIFDDHPLQRCFQDIHALTQQVQGRASHYRSVGRVLLGRQKETFAF"
     gene            complement(3923..4324)
                     /locus_tag="AC16VR_05470"
     CDS             complement(3923..4324)
                     /locus_tag="AC16VR_05470"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6010640.1"
                     /translation="MKITRINHAAVNVTGKVEEAHDFYVKLLGLPQVPIQLPGAPPVM
                     ESALGFWVEQDGVQLHVIGREPFGGTPDPTQNHVSWFVEDLDVAVGELEQNGIEMRVM
                     GEGSGRIIWVLDPGGNIVEFQQDPDLAPPPA"
     gene            complement(4406..5605)
                     /gene="glcF"
                     /locus_tag="AC16VR_05475"
     CDS             complement(4406..5605)
                     /gene="glcF"
                     /locus_tag="AC16VR_05475"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HIR6010641.1"
                     /translation="MQIELHDDFAETADGQRAQDILRACVHCGFCNATCPTYQVTGDE
                     LDGPRGRIYLIKEMLEEGSANRVAERHLDRCLTCRACETTCPSGVAYGELLEVGRELV
                     ERDRRRDPIASFLRAWLLAVVPDPGRFRRWARLGGWFRWLLPGRLGAALPKVGPEQPV
                     SRTGASRSVLVLQGCVQRTATPGVNAALIDLLNARDYDVVYARNEGCCGSLNLHLAEK
                     ERALADARRLLDVVAEDLEEVEAVISTASGCGVTLKDYPRLLADDPAYADRARALAGK
                     LVDAAEFFASRELELERAFDGLRVAWQAPCTLQHGQKITGMVEKVLGAAGYELVSVVD
                     PHLCCGSAGTYSILQPEMAGELRRRKLATLCAQTPDVIATANVGCQTHLAAESPVPVR
                     HWLELLR"
     gene            complement(5595..6653)
                     /gene="glcE"
                     /locus_tag="AC16VR_05480"
     CDS             complement(5595..6653)
                     /gene="glcE"
                     /locus_tag="AC16VR_05480"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: protein motif:HMM:NF008439.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HIR6010642.1"
                     /translation="MAGLDRDDSERLVEAVRDAYARNQPLAIVGSGSKSFLMPADDGA
                     RLLSVAEHAGVLEYRPDELVVTARAGTPLKDLQQLLSQHRQMLPFEPPLFGGGGTLGG
                     ALAAGLSGPGRPWRGSARDALLGVELINGRGERLRFGGKVLKNVAGYDLSRLQGGAFG
                     TLGVLLAASVRVVPAPQAERTCLLDMPAAPALQTVRALCARPVPLTATCFAEGSLRLR
                     FSGSEAAVLSASRDAGGELVDGDRYWQDLRDHRLPVMRPDVPLWRVSLPPGADPALQD
                     GVVEWGGALRWLVAVDPGPGELAAGLGGWAARFGAGYARAVCDALPPPQMKYQRRLKR
                     NFDPENLFNPGLVGTDAD"
     gene            complement(6654..8108)
                     /locus_tag="AC16VR_05485"
     CDS             complement(6654..8108)
                     /locus_tag="AC16VR_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014590532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HIR6010643.1"
                     /translation="MNHASALRQLRAALPEGAVLTDRHQLKPYETDGLTALRQMPWAV
                     ALPQTEAEVREVLRICAEHEVPLVPRGAGTGLSGGARPVSDGLVLGVSRLDRILEIDV
                     ENGVARVEPGVRNLAISEAASAYGLYYAPDPSSQIACSIGGNVAENSGGVHCLKYGLT
                     VHNVLGLKVLTVEGDELQIGGKRLDSPGYDLLALMSGSEGMLAVITEITVRLLPIPPE
                     KRVLLGAFRTVAAAAEAVNQVISEGLVPAGLEMMDRLAIEAAEDFVHAGYPREAEAIL
                     LCEVDGVTEEVDNDVDLVRRVFETLGAFDVRIATDPAEQANLWKGRKAAFPAVGRISP
                     DYYCMDGTIPRYELARVLAEIASLATEFGLRVANVFHAGDGNLHPLILYDAGREGELA
                     KAEALGGKILELCVEVGGTVTGEHGVGVEKLDQMCAQFSPEELAQFFGVKACFDPGQT
                     LNPGKAIPTLARCSEVGGMHVHGGRLPYGNLERF"
     gene            8243..9571
                     /locus_tag="AC16VR_05490"
     CDS             8243..9571
                     /locus_tag="AC16VR_05490"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019580362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIR6010644.1"
                     /translation="MTNSYLISNARIVNEGTITEGDLLVENGRISGIGKPAPAGTDVF
                     DANGAWLLPGMIDDQVHFREPGFEHKGTIATESRAAIAGGITSYMEMPNCNPLTVTHE
                     ALADKHVRAKDHSAANFAFYFGATNDNLEAIKTVDPALTCGIKVFMGASTGNMLVDDA
                     DTLEGIFAGTPLIVATHCEDTPTILANEERAREAFGDAIPFSEHPRIRSEEACWLSSS
                     MAVGLARRHGTRLHVLHLTTAREMALFEPGDLMGKQITAEACVHHLFFNESWYPDKGA
                     DIKCNPAIKGAADQEALLRAVAEDRIDVIATDHAPHTREEKDREYLRAPAGLPLVQHA
                     LSSLVEQVHRGVFDIETVVRKTAHAPALLFGVKERGFIREGYHADLTVIDGDALVDGG
                     EIHSKCGWSPFSGIDLHGRVRATWVNGVLRYRDGRILDGPSGIALEFERP"
     gene            complement(9574..>10167)
                     /locus_tag="AC16VR_05495"
     CDS             complement(9574..>10167)
                     /locus_tag="AC16VR_05495"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HIR6010645.1"
                     /translation="FHVVPSLSAALKAVACGVDGLVVEGGEGGGFKNPREVSTMVLLP
                     LVRSKVDVPIIAAGGFVDGASMAAAFALGAEGVQLGTRMVSALESPVHQNWKDAIVNA
                     GETDTVFLNRKHSPALRALRTEKTTRLENEPDTNAMSEFGSALDLYFGGDMEASIALS
                     GQVCGRIDAVRPVAEIIAELKDEFFGTIARLDEQYLS"
BASE COUNT         1822 a         3590 c         3202 g         1553 t
ORIGIN      
        1 acgcaaagcc ctgggtctgc tgggtctcgc cgttgtgctg gcgctggcgg cactcacagc
       61 ctatctggtc tatctggacc ggacgatcac caccaccttc gaaggtcgac gctggacgga
      121 gccggccgtc atctacgccc ggcccctgga gctccatccc ggcgctgcgc tcaccaccgc
      181 cgaggtgatc gccgaactcg accggctggg ctacgcgttg cagccatccc cgccgaaacc
      241 cggcgcttac caccgaagcg gagacgagct ggacgtttac ctgcgcgcct tcgactttcc
      301 caacggatcc cgggacgccc agcggataac gctcaaattc tctgccgggt ccctggcgcg
      361 ggttcaggac gccctcggcc gtccggtgcc cttgatccgg ctggacccgg tcaccatcgg
      421 cagcttcttt ccttcccacg gcgaggaccg cctggtgctc ccgcccgagc gggtacccga
      481 tctgctgcgt gaaaccctca aggtcgtaga ggatcgcaac ttcgaccgcc acgccggttt
      541 cgacctgcgt gccatcgccc gagcgttctg ggtaaacgtc accgccgggg aggtcagaca
      601 ggggggcagc acgctgaccc agcagctggt gaagagctat ttcctcgaca accggcgcac
      661 catcggccgc aagctgcgtg agctcgccat ggcgatcatc ctggacgccc gcttcggcaa
      721 ggaagatctc ctcaacgcct acgtcaacga gatttacctg ggtcaggacg ggcgccgcgc
      781 cgttcacggc ttcgggctcg gcgcccagtt ctacttcaac cgtcccctga cagagctgga
      841 catccaacag cttgcgctgc tcatcgcggt gatcagaggc ccctcctact acgacccttt
      901 tcgacatccc gagcgggcgc gggcgcgccg ggatctggtc ctcgacaaga tgcttcaggg
      961 cggcctcgtc gacgaagacc agcacgcacg ggcagtgcga tcacccctga ccacggtcgc
     1021 gggcacccgc cgcggcgggg cctactatcc ggccttcctg gatctggtcc gcgccaacct
     1081 gaccgccctc gaccgcgatg cgttgaccac ccggggcctt gcggtcttca cgacgctcga
     1141 gccccagacc caggatgacc tggagcaagc ggtggctcag gctcttgggg atctggagtc
     1201 cagccggggg ctggaacccg gctccctgca ggcggccgcc gtcatgaccg ccacccagac
     1261 cggcgaggtg ctggccttga gcggcgcgcg cagcgcggga agcgacggtt tcaaccgggc
     1321 gctcaatgcc aggcggccgg tcgggtccct gctcaagccc gtggtctatc tggcggcgct
     1381 ggagcagggg cttcatctgg ccagcctggt cgacgacgcg cccgtcgtgc tcccgcttgc
     1441 cggcggcacc acctggcaac ccgaaaactt cgatcacgtg acccacggag aagtccccat
     1501 gatccgggca ctggccgagt cgctgaatct cgccacggtc aacctgggac tcgaggtggg
     1561 cgtggggacg gtggccgccc gcttcgagac tctcatcggc cgcccacccg caaacacgta
     1621 tccttcgctt ctgctcggtg cggaggcaat gacgccggtg gaggtatcgg cgctctacgg
     1681 catcttcgcc agcggcggct tctaccagcc gcccaaagcc gtgatcagcg tgctcgacga
     1741 atcccgaagc cccacgttga ggcgccccct caagctcgag cagcgcatcg acacgaaaac
     1801 ggcgcgagcc ctcgaccggg cgctgcaagt cgtcatggcg cgcggaacag ggcgtagctc
     1861 acgcttcgcc agggtcggcg tagcgggcaa aacgggcacc tcagacgact atcgtgacag
     1921 ctggttcgcc ggatacgatg acgccgcgct gacagtggta tgggtgggcc gcgacgacaa
     1981 cgcgccgacc ggcatgaccg gcgcgtccgg cgcgctcaag gtctgggacc ggatcatggc
     2041 ggatcgcgac gtgatcgcca ttccgccgga tccggattcg cggaaggtgg acgtggaatt
     2101 cgcaaccggc ctcgtggcca acccgctctg cgcggacgtg gtcaccatac ccgttcccga
     2161 gggagctgct ttacaggaaa aaccgggctg tggtatcaag ccgccgtctt tgggtgagcg
     2221 cattcgcaat tggctacgaa acgactgatc ccaggcctgg caacgctgat cgtgactgca
     2281 ggttgcgcca cgtcctccgt gctgcctccc gtggtggaca ccaacggcat cgaggacgcg
     2341 gagccggcgc cccagcagga agtcccgatc ccaccggcga gcacccccga ggcccggcag
     2401 ccggacacgg ccggggccac cctggcgctg ctcgagcaga gcgagcgcgc cgcatcggcc
     2461 ggcgacgcgg atgccgccgt cgcctacgtg gagagggcca ttcgcctgaa tccccgcagc
     2521 gccgacctct ggctgcagct cgcccgcttg cagctcgcca acgaggatcc ggaagcggcc
     2581 gtgcagttcg ccaacaaagc catcagcctg gccggcgatc gcctcgactg ggtacgcgat
     2641 gcctggctgc tgatcgcaga cgcccgagcg gctcagggcg atggggatgg cgccagggag
     2701 atccgcgagc gctggagagg ctatcgcggt tgagcagggc cctgggtcct cagaaagcga
     2761 atgtctcttt ctgcctgcca agcagcacac gacccaccga acgatagtga gaagccctgc
     2821 cctgaacctg ctgggtcagc gcgtgaatgt cctgaaagca gcgctgcagc gggtggtcgt
     2881 cgaagatgca gtccgtgcct gccatggcgt aaacccgatc gaccacgtcc gccgagcgac
     2941 gcatggcgtg ggtcgcggca aggcgcatcc gggatctgag ttcgaaatcg acggctccgg
     3001 tggttgcatg gcgatgctcg cactcctcgg cggcatccag caggaaagcc cgggtggcgc
     3061 cgagcagcgc ttccgcctcg ccgagcccgc tctgcacgag gtcgtcctcg gccagcttcc
     3121 ggttcgcaaa cacgggcgtc tttccggcgg ccagggcgat gaactcatcc actgcccgtc
     3181 gagcaacgcc cagccccacc gccgcgaacc cgcaggcgaa caggagcatg acgtgcagcc
     3241 cggtggaggc accgggatga ctggtgtagg catccgcaac ggatcgccgg ctcggcacgt
     3301 gcagcgcatg caaccggtac tcacgactgc ccgttccttg catcccgcgc acgttccatc
     3361 cttcgccgag acgaacctcc tcgcggggga cgagaaaagt gcgcgacggc tcgctcccga
     3421 catcgccaac cacggaggcc aggggtgcaa accagtcggc atgggtgcag ccactggcaa
     3481 aacgccagcg gccatcgagc aagtagccgc catcgaccgc tcgggcctcg gcaccgaacg
     3541 gaatgcctgt ggcaacggca gcgtcgcggt tggtccccca gatctcagcg gccgtttccg
     3601 catgcagcag atcggcaacg ctggcaaaca ccgccgcctg agaaacgcac cagcccgtgc
     3661 tgccgtccgc ttcggcaatc acttccacca cgcgcacgaa ccgcggcaac gacaacccgg
     3721 aaccgccgat acagaccggc agaagcagtc ggaacatgcc gtcagcggca agccgagcgg
     3781 cgaggtcagc cggcagcgcc tgctgcttgt cgatctcgca ctgcgaggtc cgtatgccgg
     3841 gcaccaatcc cctggcgctc tcgagctcgg caaacgactc ccggcggctt tcgaacctca
     3901 cgaggacttt ccgccagtac gatcaggccg ggggaggagc aaggtccggg tcctgctgga
     3961 actccacgat gttcccgccg gggtcgagaa cccagatgat ccgacctgaa ccctcgccca
     4021 tcacccgcat ctcgatgccg ttctgctcga gctcgccaac cgccacatcc agatcctcca
     4081 cgaaccagga aacgtggttc tgggtcggat cgggcgtccc accgaagggc tcgcggccga
     4141 tgacgtgcag ctgcacgcca tcctgctcca cccagaaacc gagcgcagac tccatgaccg
     4201 gcggcgcgcc cggcagctga atgggcacct ggggcaaccc gagcagcttg acgtaaaaat
     4261 cgtgagcctc ttccaccttg ccggtgacgt tcaccgccgc atggttgata cgcgtaatct
     4321 tcatgaatct cccctttatg ccacctcgcg tttcatttaa gtggactgcg cctaccaccg
     4381 caatccccgc tcccatggac gggccctagc gcaggagctc cagccagtgg cgaacgggca
     4441 cggggctctc cgcggccagg tgggtctggc aacccacgtt ggccgtcgcg atcacgtcgg
     4501 gcgtctgcgc gcacagcgtg gcgagcttac gccggcgaag ctcgccggcc atctccggct
     4561 gcagaatgga gtaggtcccg gccgagccgc agcacaagtg ggggtccaca acggagacga
     4621 gttcgtagcc ggccgcgccg aggaccttct ccaccatccc cgtgatcttc tgcccgtgct
     4681 gcagggtgca cggcgcctgc catgccactc gcaagccatc gaaggcacgt tcgagctcca
     4741 gctcccggga ggcaaagaac tcggcggcgt cgacgagctt tcccgccaga gcccgcgcgc
     4801 gatcagcgta agcggggtcg tccgccagca accgggggta gtctttgagc gtcacgccgc
     4861 agccgctggc cgtggatatg accgcttcca cctcttcaag gtcctctgca acgacatcga
     4921 gcaggcggcg ggcgtccgcc agggcccgtt ccttttccgc aagatgcaga ttcaggctgc
     4981 cgcagcagcc ttcgttgcgg gcgtagacga cgtcgtagtc cctggcattg agcagatcga
     5041 tcaatgccgc gttgacgccc ggcgtggccg tgcgctgaac gcagccctgc aggacgagca
     5101 cgctgcgcga cgcgcctgtc ctggaaacgg gctgttccgg gcccaccttc ggtagcgcgg
     5161 cacccagacg tccgggaagc aaccagcgga accagccgcc cagacgcgcc cagcggcgaa
     5221 agcgcccggg gtctgggacc acggcaagca gccaggcgcg caggaacgaa gcgatgggat
     5281 cgcggcggcg gtcacgctcc acgagctcgc gacccacctc cagcagctcg ccgtaagcga
     5341 cgccgctggg acacgtggtc tcgcaggctc tgcaggtcag acagcgatcc agatgacgct
     5401 cggcgacgcg gttggccgag ccctcctcca gcatctcctt gatcaggtaa atacggccgc
     5461 gcggaccgtc cagctcgtcc cccgtgacct gatacgtcgg gcacgtcgcg ttgcagaaac
     5521 cacagtgaac gcaggcgcgc aggatgtcct gcgccctctg cccgtcggcc gtttccgcga
     5581 agtcgtcgtg cagctcaatc tgcatctgtg cccaccaatc ccgggttgaa caggttttcc
     5641 ggatcgaagt tccgcttcag ccgccgctga tatttcatct gcggtggggg cagggcgtca
     5701 cagacggcac gggcgtatcc ggcaccgaat ctcgccgccc agcctccaag gccggcggcc
     5761 agctcccccg gacccggatc gacggcgacg agccagcgca gcgcgccgcc ccactccacc
     5821 acgccgtcct gcagcgccgg gtctgctccc ggcggaaggc tcacacgcca caaggggacg
     5881 tcggggcgca tgacgggcag tcggtggtcc cgcagatcct gccagtagcg gtctccgtcg
     5941 accagctctc cgccggcatc gcgagaagcg ctcagcacgg cggcctcgga cccggagaag
     6001 cgcagccgca ggctgccctc ggcgaaacag gtggcggtca gcggtaccgg gcgggcgcac
     6061 agcgcgcgca ccgtttgcaa cgctggcgcg gccggcatgt ccagaaggca cgttcgctcc
     6121 gcttggggcg ccggcaccac ccgcacgctc gcggcgagca gcacgccgag cgtgccgaag
     6181 gcgccaccct gcaggcgcga aaggtcgtat ccagcgacgt tcttgagaac ctttccacca
     6241 aaccgcagcc tttcgccgcg cccgttgatc agctcgacgc cgagcagcgc gtcccgggcg
     6301 gacccccgcc acggccgtcc ggggccggaa agaccggcgg ccagagcacc gcccagcgtg
     6361 ccgcccccac caaacagcgg aggttcgaac ggcagcatct gccggtgctg cgacaacagc
     6421 tgctgaagat ccttgagcgg ggtgcctgcc cgggcggtga cgaccagctc gtccggccgg
     6481 tactccagca cgcccgcatg ctccgcaacg gagagcagcc gggcgccatc gtccgcgggc
     6541 atcagaaacg acttgctgcc ggaaccgacg atggcgagcg gctggttcct ggcatacgcg
     6601 tcgcgcaccg cctccaccag acgttcgctg tcatcccggt cgaggccggc caactagaac
     6661 cgctccagat tgccgtacgg gagacgaccg ccgtggacgt gcatgccccc gacttctgag
     6721 cagcgcgcca aagtcgggat cgccttgccc ggattgaggg tctggccggg atcgaagcag
     6781 gccttcaccc caaagaactg ggcgagttcc tcgggcgaga actgcgcgca catctgatcg
     6841 agcttctcga cgccaacacc gtgctctccg gtcaccgtgc cgcccacctc gacgcaaagc
     6901 tcgaggatct tgccgccaag ggcctcggct ttcgcgagct caccttcgcg ccccgcgtcg
     6961 taaagaatca atgggtgcag attgccgtcg ccggcatgga agacgttggc gactcgaagc
     7021 ccgaactccg ttgccagcga agcgatctca gccagcacgc gcgcgagctc atagcggggt
     7081 atggttccgt ccatgcagta gtagtccgga gagatacgcc ccaccgcggg aaaggcggct
     7141 ttgcggccct tccacagatt ggcctgctcg gccggatcag tggcgatgcg cacgtcgaaa
     7201 gcaccaagcg tttcgaacac cctgcgaacc aggtcgacgt cgttgtccac ctcctcggtc
     7261 acgccgtcga cctcgcacag cagaatggct tccgcctccc gcgggtaacc ggcgtgcacg
     7321 aagtcctcgg ccgcctcgat ggcgagccgg tccatcatct ccaatcctgc cggcaccaga
     7381 ccctctgaga taacctgatt cacggcctcc gccgccgctg cgaccgtgcg gaacgccccg
     7441 agcagcaccc gcttttccgg cggtatcggc aggagccgca cggtaatctc ggtgatcacc
     7501 gcgagcatgc cttccgagcc cgacatcagc gcgagcagat cgtaaccggg agagtctagg
     7561 cgcttaccgc cgatctgcag ctcgtcgcct tcgacggtga gcaccttcag ccccaaaacg
     7621 ttgtgaacgg tgaggccgta cttcaggcaa tgaacgccgc cggagttttc cgccacgttg
     7681 ccgccgatgc tgcaggcgat ctgggaggaa gggtcgggcg cgtagtaaag cccgtaagcg
     7741 gatgctgcct cgctgatggc caggttgcgg acgcctggct cgacccgggc cacaccgttt
     7801 tccacgtcga tttcgagaat tcgatccaac cgggaaaccc ccagcaccag gccgtcgctg
     7861 acgggcctcg ccccgccgga gagcccggtc ccggcccccc gcggaaccaa aggcacctcg
     7921 tgctcggcgc agattcgcag cacctcccgc acctctgcct cggtctgtgg aagcgccacg
     7981 gcccagggca tctgcctgag cgcggtgagc ccgtccgttt cgtagggctt gagctggtgc
     8041 cgatcggtga gaacggctcc ctcgggcagg gcggcacgca gctgtcggag agcgctggca
     8101 tggttcatgg tggaggagta tacgccatgg aagacggcgt ctttcacgtc ggcgagcgca
     8161 tcctgcccgc caaatcggct aaagtaaccc caacgcagaa accgcccacg ggaagcaccg
     8221 cgcggcgaac ggaccagact tcatgaccaa cagctatctc atcagcaacg cccggatcgt
     8281 caatgagggg accatcacgg aaggtgatct cctcgtcgag aacggccgaa tctccggcat
     8341 cggcaaaccg gcaccggcag gcaccgacgt tttcgacgcc aacggcgcct ggctcctgcc
     8401 cgggatgatc gacgaccagg tgcactttcg agagcccggt ttcgagcaca agggaaccat
     8461 cgccacggaa tcccgcgcgg ccattgccgg aggcataacg agctacatgg agatgccgaa
     8521 ctgcaacccg ctgacggtta ctcatgaggc gctggccgac aagcacgttc gcgcaaagga
     8581 ccattccgcc gcgaatttcg ccttctactt tggcgccacc aacgacaacc tggaggccat
     8641 caagaccgtg gatcccgccc tcacctgcgg aatcaaggtc ttcatggggg cgagcaccgg
     8701 caacatgctg gtggacgacg ccgacacctt agagggcatc ttcgccggca cgccgctgat
     8761 cgtggccacc cactgcgagg acacgcccac catcctggcc aacgaggaac gcgccaggga
     8821 agcgttcggc gacgccatcc cgttctcgga gcaccctcga ataaggtccg aggaagcctg
     8881 ctggctgtca tcgtccatgg ccgtcggtct cgcccgcaga cacggcaccc ggctccacgt
     8941 gctgcacctg accacggcgc gcgagatggc gcttttcgag ccgggagacc tgatgggcaa
     9001 gcagatcacg gccgaagcct gcgtgcacca cctctttttc aacgagagct ggtaccccga
     9061 caagggtgcc gacatcaagt gcaaccctgc catcaagggc gccgctgacc aggaagccct
     9121 cctgcgcgcg gtggcggaag accgcatcga cgtgatcgcc accgatcacg ccccccacac
     9181 ccgggaggag aaggatcgcg agtacctgcg cgccccggcc gggctgccgc tggtgcagca
     9241 cgccctttca tcgctggtgg agcaggtcca ccgcggcgtg ttcgacatcg agaccgtggt
     9301 ccgcaaaacg gcccacgcac cggcgctgct cttcggcgtc aaggagagag gcttcatccg
     9361 cgagggatac cacgccgacc tgaccgtgat cgacggcgat gccctggtgg atggcgggga
     9421 gatccactcc aagtgcggct ggagcccgtt cagcggcatc gatctgcacg gcagggtccg
     9481 ggcaacctgg gtgaacggcg tgctccgtta ccgcgacggc cggatcctcg acggcccgag
     9541 cggcatcgcg ttggagttcg agcggccctg aactcagctc agatactgct cgtccaggcg
     9601 cgcgatggtc ccgaagaact cgtctttcag ctcggcgatg atctccgcca ccgggcgaac
     9661 ggcgtcgatg cggccgcaaa cctgacccga cagagcgatg ctcgcttcca tgtcgccacc
     9721 gaaatagaga tccagcgcgg agccgaactc gctcatggcg ttggtgtccg gttcgttctc
     9781 cagccgcgtc gtcttctccg tgcgcagcgc tctcagcgcg ggcgagtgct tgcggttgag
     9841 aaacaccgtg tccgtttcgc cggcattcac gatggcgtct ttccagttct gatgcacggg
     9901 tgactccagc gcggacacca tccgcgtgcc gagctgaacg ccctccgcgc cgagcgcgaa
     9961 cgctgccgcc atggacgcgc cgtccacgaa cccgcccgcg gcgatgatgg gaacatccac
    10021 cttcgagcga accaggggca gcagaaccat ggtggagacc tcgcgtgggt tcttgaagcc
    10081 gccgccctcg ccgccttcca ccaccagtcc atccacgccg caggccacgg ccttcaacgc
    10141 agcgctgagg ctgggaacca cgtgaaa
//
LOCUS       DBNHQR010000237         2492 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_212895, whole genome shotgun sequence.
ACCESSION   DBNHQR010000237 DBNHQR010000000
VERSION     DBNHQR010000237.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_212895"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="AC16VR_05500"
     CDS             <1..518
                     /locus_tag="AC16VR_05500"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003152943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase domain-containing
                     protein"
                     /protein_id="HIR6010646.1"
                     /translation="VVAWTGNSAPYKYDLGRFNVMGSVSFDHPDPSIYTVLTSPSETA
                     GMANADFVIFPPRWIVAEDTFRPPWYHRNVMSEFMGLVRGLYDAKETGFVPGGCSLHN
                     SMAAHGPDVEAFEKASSADLKPQKLADTLAFMFESRYPYTATAFAMESPALQSDYRAC
                     WAALPRMFEPQ"
     gene            564..1772
                     /locus_tag="AC16VR_05505"
     CDS             564..1772
                     /locus_tag="AC16VR_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6010647.1"
                     /translation="MVPTETEIREFDLRNPPAGFVEDPFFWYEALRAGGPVRRMPDGS
                     MLVTGYDACSDIYRNEAFISDKRNLFRPKFGDSPLYEHHTTSLVFNDPPYHTRVRRTI
                     VEALKPRSIQPTLDMLARRIQELLASMGEAGEADLIEAYASRIPVEVICSLLTVPTQD
                     RDDLRRWSLAILGALEPAISESEAEQGNRAVTEFLGYLRTLIAERRRRPLAEDSVLSS
                     LLAQQDRGEVSELELLHNCIFLLNAGHETTTNLIGNGVHMLLTHDSERDLLVAHPSRI
                     KSAVEEILRYQSPNQLGNREVTGNVTMRGQRFAPGDQVTLCIAAANRDPRKFPDAHRF
                     LIDRAPNPHLAFAAGPHTCAGMALARIEGKLALSAFFQAFPEATLQARPRYRDRIRFR
                     GLEALHVRLQ"
     gene            1857..>2492
                     /locus_tag="AC16VR_05510"
     CDS             1857..>2492
                     /locus_tag="AC16VR_05510"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA dioxygenase family protein"
                     /protein_id="HIR6010648.1"
                     /translation="MTEAFDVVGDWQVARLAGALGAEVRGIDLRQVTSEEVDRIRSLL
                     LDHLVLFFPDQNISVDDHVSFGRYFGELEGHPHLDNPFTEHPELFELAASRGGVADEW
                     HTDITFRDEPALMSILHMVRCPTVGGDTMWSSLYAAFEELSEPMRDLCEGLTALHDAL
                     PHGRPEQTAIHPVVRVHPETGRKALYVNEHFTRRIVEMNARESDMLLGYLTK"
BASE COUNT          448 a          781 c          801 g          462 t
ORIGIN      
        1 acgtcgtggc ctggacaggc aactcggcgc cgtacaagta cgatctgggc cgattcaacg
       61 tcatgggctc ggtgagcttc gatcatcccg atccgtccat ctacacggtg ctcacctcgc
      121 cctccgagac cgcgggcatg gcgaacgcgg acttcgtgat ctttccgccg cgctggatcg
      181 tcgccgaaga cacctttcgt ccgccctggt accaccgcaa cgtcatgagc gagttcatgg
      241 ggctggtgcg gggtttgtac gacgccaaag agaccggctt cgtgcccggc gggtgcagcc
      301 tgcacaacag catggcggct cacggtcccg atgtggaggc cttcgagaag gcgagctcgg
      361 cggatctgaa gccgcagaag ttagccgaca cgctggcgtt catgttcgag tcccgctatc
      421 cctacacggc taccgcgttc gccatggaaa gcccggccct gcagagcgat taccgagcct
      481 gctgggcggc gctgcctcgg atgttcgagc cgcagtgatg cgcacgggga tggcggccgg
      541 cccacgggcg gggtgagatg ccgatggtgc cgacggagac ggaaattcgt gaattcgacc
      601 tgcgcaaccc gccggccggg ttcgtagagg atcccttttt ctggtacgaa gcgcttcgcg
      661 ccggcggtcc cgtccgccgg atgcccgacg ggtcgatgct ggttaccgga tatgacgcgt
      721 gctcggacat ctatcggaac gaggcgttca tttcggacaa acgaaacctc ttccggccga
      781 agttcggaga ctcgccgctg tacgagcatc acacgacgag tctggtcttc aacgaccctc
      841 cgtaccacac gcgggttcgg cggaccatcg tggaggcgtt gaagccgcgc tcgatccagc
      901 cgaccctaga catgctcgcc cggcgcattc aggaactgct cgcatcgatg ggggaggcgg
      961 gagaggcgga cctcattgaa gcgtacgcgt cccggattcc ggtggaagtc atctgcagcc
     1021 tgctcacggt gccgacgcag gacagggacg acctgcggcg ctggtcgctg gccattctgg
     1081 gtgcgctgga gcccgctatc tccgaatccg aggcagagca aggcaaccgc gcggtaacgg
     1141 aatttctggg gtatctcagg acgctgatcg ccgagcgtcg gcgccgcccc ctcgccgagg
     1201 acagcgttct gtcctcgctc ctggctcagc aggatcgggg cgaggtgagc gagctcgaac
     1261 tgctgcacaa ctgcatcttt cttctgaacg ccggacacga gaccacgacc aatctgatcg
     1321 gcaatggcgt tcacatgctg ctgacccacg actccgaaag ggacctgctg gttgcgcatc
     1381 cttcacgaat caagtcggcg gtggaggaga tcctgcgcta tcaaagcccc aaccagctcg
     1441 ggaaccgtga ggtgacgggg aacgtaacga tgcgtgggca acgctttgcg cctggtgatc
     1501 aggtgaccct gtgcatcgca gcggcgaatc gcgacccgcg caaatttccg gatgcccatc
     1561 gtttcctgat cgatcgcgcg ccgaatcccc acctggcctt tgccgcgggt ccccatacct
     1621 gcgcgggcat ggcccttgcg cgaatcgagg gaaagcttgc gctcagcgca tttttccagg
     1681 cgtttcccga agcgaccctt caggcgcggc cacggtatcg tgatcggatc cgcttccgcg
     1741 gcctggaagc gctccacgtc cgcttgcagt gaacgcggca caaggggcct tggcggcttg
     1801 caggcccgga agcgttatat actctgccta cgtgacgcgg aatctgatgg acgatcatga
     1861 ctgaagcttt cgatgttgtc ggcgattggc aggtggcacg gctggctggc gccctgggcg
     1921 cggaggttcg cggaatcgat cttagacagg tgacgtcgga ggaggtggac cggatccgga
     1981 gcctgttgct cgaccacctg gtcctgttct ttcccgatca gaacatcagc gtagacgacc
     2041 acgtctcgtt cggacggtat ttcggtgagc tcgagggcca tccccatctg gataacccgt
     2101 tcaccgagca ccccgagctc ttcgagctgg cagcgagccg cggcggcgtc gccgacgagt
     2161 ggcacacgga catcactttt cgggacgagc ccgcgcttat gtcgattctg cacatggtgc
     2221 gatgcccaac cgttggcggg gacaccatgt ggtcgagcct ttacgcggcg ttcgaggaac
     2281 tctcggaacc catgcgcgat ctctgcgagg gcctcacggc cctgcatgac gcgctgcccc
     2341 atggccgccc ggagcagacg gccattcatc cggtggtccg cgtgcatccg gaaaccgggc
     2401 gcaaggcgct ttacgtgaac gagcatttca cccggcgaat cgtggagatg aacgccaggg
     2461 aaagtgacat gctgctgggc tatctcacga ag
//
LOCUS       DBNHQR010000238         4842 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_846946, whole genome shotgun sequence.
ACCESSION   DBNHQR010000238 DBNHQR010000000
VERSION     DBNHQR010000238.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4842)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_846946"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1980)
                     /locus_tag="AC16VR_05515"
     CDS             complement(<1..1980)
                     /locus_tag="AC16VR_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR6010649.1"
                     /translation="MIRQALPKTVWTVTCVLTLTACGGGGGGSDTPTPAPANRSPAAS
                     FTATPTEGTVPLSVDFDASGSSDADGRIASYAWTFGEPPGASSTSGVNTSHTYEEAGT
                     YTVELTVTDDDGATGSTTRTVTVEGPVASISGTVRILASSAIDSDVNDVGSTPAPNND
                     FASAQPLPNPVTLGGFANVAGVGVGDEPERGKLFQDGDPGDFYAMTMVGNEFIYLNIA
                     DPENAEQELLLYDSQQNVISADGGPGEVQVAEVPAAGDYFIEVRPIAGASNYVLTVGQ
                     DLVPERATTMIDPFLPREIILRTTDDEIAAEYGLSMLAQSGPRMLLKAGPATAALRHI
                     DRRAPFDASWTEDQQDRYATLAVIEALGRDPRVELAEPNLIRKPHREPGDSFYRSQWH
                     YQAINLPLAWDITTGDGDVIVAVVDTGIRADHPDLDEKVIAGFDFIANLQRAADGDAR
                     DDDPTDPGDGAFGGSSSFHGTHVAGTVAAETSASVTPASGVAGVAWEAKIMPLRVLGV
                     GGGTSFDVIESMRWAARLDNVSGTLPDRAADVINLSLGGGGFLESEQQAIDEIRAAGT
                     IIVASAGNDASASPSYPAAYDGVVSVSATTIENNPAAYSNFGTTVDVAAPGGSNVTDV
                     NGDGIGDGVISTMADDSDPSSLRFGYASLQGTSMAA"
     gene            complement(2417..3319)
                     /locus_tag="AC16VR_05520"
     CDS             complement(2417..3319)
                     /locus_tag="AC16VR_05520"
                     /inference="COORDINATES: protein motif:HMM:NF024803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR6010650.1"
                     /translation="MVSRSAIDDDLYRIGTVATLTGISVERLRAWERRYGLAPTYRSG
                     KTRFYSKQQLQTLQRIKRLIDQGHPISSLAPLSDEQLDERLSAATPRLQALQSATVGL
                     IGPNLLVLEQQQGSDNRLEVRARWANMDAFMSDQTGTERIDVIVAQLPVLLVDHINNL
                     ARFHPESRIVAIYQFATPAQIAAVQELGIPTLTWPAGWQEIEHACATTAGLPLRAART
                     AVRRFSDAELIAISASEHGDPAACPQHLVELISGLNAFAEYTLNCADEAAEPEVYQRV
                     HTDTTQARAQLELALEMMVDQEAG"
     gene            3745..>4842
                     /locus_tag="AC16VR_05525"
     CDS             3745..>4842
                     /locus_tag="AC16VR_05525"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6010651.1"
                     /translation="MENNLGLFLTKRAFLTPQREGYVDSHSGLRLTFRQLNERSNQLA
                     NSLLQAGVQKEERVGLLLMNSAEFMEAYFALAKIGAVVVPLNWRLVADELEFILKDSG
                     TARLIFDEDFVDTVAELHARGDRTDIRHWLQVEGSQEVAHFAESYEAFRDNGAAEEPP
                     CTACDDDMLYIMYTSGTTGLPKGVVHTHTTAIWAILTIAANAYYEETDRYLGALPMFH
                     VGALTPLAVNVYRGATSVVMRAFDPVLAWELIDREKITSGLMVPAMLNFMLQVGEAKD
                     RYDYSALRWIMTGAAPVPVALTEQYVQMGISILQVYGLTESCGPACLMDSENALRKPD
                     STGKAFFHTDVRVVDAHGNDCAPGEAGEVVVR"
BASE COUNT          802 a         1461 c         1652 g          927 t
ORIGIN      
        1 cgcggccatg gacgtcccct gcagggatgc gtagccgaag cgcaggctcg aggggtcgct
       61 gtcgtcggcc atggtgctga tgaccccgtc tccgatcccg tcgccgttga cgtcggtgac
      121 gttggacccg cccggtgccg ccacgtcgac ggtggtcccg aagttcgaat acgccgccgg
      181 attgttctcg atggtggtgg cgctgacgga gacgaccccg tcgtaagcgg ctggatagct
      241 gggcgaggcg gacgcatcgt tgccggcgct ggcaacgatg atggtgcccg ccgcgcggat
      301 ctcgtcgatg gcctgctgct cgctttcgag aaacccgccc cctccgagac tcaggttgat
      361 cacgtccgcg gcgcggtctg gaagggtgcc ggagacgttg tcgaggcggg cggcccatcg
      421 catggactcg atgacgtcga agctggtgcc gccgcccaca ccgagaaccc gcaggggcat
      481 gatcttcgcc tcccacgcga cgccggcaac gcccgaggcc ggggtgaccg aggcggatgt
      541 ttccgccgcc acggttcccg caacgtgggt gccgtgaaag ctgctggaac cgccgaacgc
      601 cccgtcgccc ggatcggtcg gatcgtcgtc gcgagcatcc ccgtcggctg cccgctgcag
      661 attggcgatg aagtcgaagc cggcgatgac cttctcgtcg aggtcggggt gatccgcccg
      721 gatgcccgtg tcgaccaccg cgacgatcac gtccccatcg cccgtggtga tgtcccacgc
      781 cagaggcagg ttgatggcct ggtagtgcca ctgggaccgg tagaaagagt cgcccggctc
      841 ccggtgtggc ttccgaatca ggttgggttc cgcaagctcg acgcggggat cgcggcccag
      901 cgcctcgatg acggccaggg tggcgtatcg atcctgctga tcttcggtcc acgaggcatc
      961 gaatggtgcc cgccgatcga tgtgccggag cgcggccgtt gccgggccgg ctttcagtag
     1021 catccgcggg ccggactgag cgagcatgct gaggccgtac tccgccgcga tctcatcatc
     1081 ggtcgtgcgc aggatgatct ctcgtggcag gaacggatcg atcatggtgg tggcccgctc
     1141 ggggacgagg tcctgaccca ccgtgagcac gtagttgctg gcgccggcga tggggcgaac
     1201 ctcgatgaag tagtctccgg cggcgggaac ctcggcgacc tgaacctctc ccggcccgcc
     1261 gtcggcgctg atcacgttct gctgcgagtc gtaaaggagc agctcctgct ccgcgttctc
     1321 gggatcggcg atgttcaggt agatgaactc gttgccgacc atggtcatgg cgtagaaatc
     1381 gccgggatcg ccgtcctgga agagctttcc cctttccggc tcgtcgccca cgccgacgcc
     1441 cgctacgttg gcgaaccccc cgagggtcac cggattaggc aaaggctgcg cgctggcaaa
     1501 gtcgttgttc ggcgccggcg tgctgcctac atcgttcacg tcgctgtcga tggcgctgga
     1561 ggccagaatt cgtacggtac cgctgatgct tgcgacaggc ccctccaccg tgacggtgcg
     1621 agtcgtgctc ccggtggcgc cgtcgtcatc ggtaaccgta agttctacgg tgtaggtgcc
     1681 ggcctcctcg taggtatggg acgtgttaac gccgctggtg ctcgatgctc ctggaggctc
     1741 gccgaaggtc catgcgtagc ttgcgatgcg tccgtccgca tcgctggacc cgctggcatc
     1801 gaaatcgacg cttaaaggca cggttccctc ggtcggggtg gccgtgaagg atgccgccgg
     1861 cgaccggtta gcgggggccg gggtgggggt gtcgctgccg cccccgcccc cgccacaggc
     1921 agtcagggtt aatacgcagg taaccgtcca cacggttttt ggcaatgctt ggcgaatcat
     1981 ctttgcccac ttctgcccat cacggccggg tccgccgatt tttgcacaga tcgggtgccg
     2041 gctctgtgcg ccgggcagca cgatggcgtt gcccgggcgg tcaacgttgc gaaaaggctg
     2101 gggcgttgcg aaaaggctgg ggccggaaac gacacaggcc tctgaaagag gcctgtgcgt
     2161 taccgtgggt ctgatccggc cggctgccgg ctcagtccgt gtaaaaaagg cgtgtcagca
     2221 gtgggcaccg ctgaaacaac ttctcttctg ggggaaggac acttgaaagg gaggactgca
     2281 tgaagttgtg ttgcacgcct gaactgttag tggggctatt atgtacagta caaaatctgt
     2341 gtcaacccat ttttaataaa aatgtacagg ttttgtttag gtcgttttgt ttaggtcaat
     2401 gtcgagcctc atgggatcag cccgcctctt gatccaccat catctccaga gccagctcca
     2461 gctgcgcgcg tgcctgggtg gtatcggtgt gcacgcgttg gtagacctcc ggctcggcgg
     2521 cctcatccgc gcagttcagc gtgtactcgg cgaaggcgtt cagcccgctg atcagctcga
     2581 ccagatgctg agggcacgct gcgggatccc cgtgctcgct ggccgagatg gcgatgagct
     2641 ccgcgtcgct gaatcggcgc acggccgttc gggcggcgcg tagcggcagc ccggccgtgg
     2701 tcgcgcaggc atgctcgatc tcctgccagc ctgccggcca ggtgagggtg ggtatgccga
     2761 gctcctggac ggcggcgatc tgggccggcg ttgcgaactg gtagatggcg acgatccggc
     2821 tttcagggtg gaaacgcgcc agattgttga tgtggtcgac gagcagcacg ggcagctgcg
     2881 caacgatcac gtcgatccgc tcggtgccgg tctggtcgct catgaaagcg tccatgtttg
     2941 cccagcgggc gcggacttcc aggcggttgt ccgagccctg ctgttgctcc agcacgagga
     3001 ggttcggccc gatcaggccg accgtggccg attgcagggc ctgaagccgg ggggtggcag
     3061 cggaaagtct ttcgtcaagt tgctcgtcgg aaagcggcgc aaggctgctg attgggtgcc
     3121 cctggtcgat cagacgtttg atgcgctgaa gggtctgaag ctgctgcttg ctgtagaagc
     3181 gcgtcttgcc cgagcggtag gtgggggcca gcccgtaacg ccgctcccag gcacgcaggc
     3241 gctccaccga gatgccggtg agcgtggcga ccgtgcctat cctgtagagg tcgtcgtcga
     3301 tggcggagcg tgataccacc ctgcagcgta ccagtacaat acctgtacag aattgtacat
     3361 gctaacgtca ttttgtccag gtagccggtc tgacgccggt gcagggagtg cagagagtgc
     3421 aggaggaagg tggcgtggcg acagcgcatt gtggcgctgt cttcacggtg agggaggaag
     3481 gggagagaga caccgcgcca cgccgatttc atttgcagat ccgacgagcg ggtccgcaaa
     3541 gaaggcgcgc attctacgga tctgcaggca gtgatcaagc gcgtccccgg gttttttgtt
     3601 acgaatcttt tcacctgggc ggagactgcg accagggtgt tcacacggac gatctggttc
     3661 gggagaatgg gctcgagagg cggtcctgtg agttcctgca gggggccttg cgctttgatg
     3721 ggttactcgc aaggggaggc aacggtggag aacaatctcg gtctttttct gacgaagcgg
     3781 gcgtttctga cgccccagcg tgaagggtac gtcgacagcc attcgggcct gcgtctgacg
     3841 tttcggcagc tgaacgaacg atccaaccag ctcgccaatt cgctcctgca ggcgggcgtt
     3901 cagaaggagg aacgcgtggg tctcctgctc atgaacagcg cggagttcat ggaggcctat
     3961 tttgcgctgg ccaagatagg ggcggtggtg gttcccctca attggcgtct tgtcgccgac
     4021 gagctggagt tcatcctcaa ggacagcggc acggcgcggc tgatcttcga cgaggatttc
     4081 gtcgacaccg tcgccgagct ccacgcccgc ggcgaccgga ccgacatccg gcactggctg
     4141 caggtggagg gttcccagga ggttgcccac ttcgctgaga gctacgaggc tttccgggac
     4201 aacggggcgg ccgaggagcc gccctgcacg gcctgtgacg acgacatgct ctacatcatg
     4261 tacacgtccg gaaccaccgg tctgccgaaa ggggtcgtcc acactcacac cacggccatc
     4321 tgggcgatcc tcaccatcgc ggcgaacgcg tactacgagg aaaccgaccg ctatctcggg
     4381 gcgctgccga tgtttcacgt cggcgcgctg acacctctgg ccgtcaacgt ctatcgcggg
     4441 gccacttcgg ttgtgatgcg cgcgttcgat cccgtgctcg cctgggagct catcgatcgc
     4501 gagaagatca cctcgggatt gatggttccc gccatgctga acttcatgct ccaggtcgga
     4561 gaggcgaagg accgctacga ctactcggct ctgcgctgga tcatgaccgg cgccgcgccg
     4621 gtacccgtcg ccctgaccga gcagtacgtg cagatgggga tcagcatcct gcaggtctat
     4681 ggcctcaccg aatcctgcgg cccggcgtgc ctgatggatt cagagaacgc gctgcgcaag
     4741 cccgactcca cgggtaaggc cttcttccac acggatgtgc gggtggtcga cgcccacggc
     4801 aacgactgcg cgccgggcga ggccggcgaa gtggtggtgc gg
//
LOCUS       DBNHQR010000239         7252 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1162258, whole genome shotgun sequence.
ACCESSION   DBNHQR010000239 DBNHQR010000000
VERSION     DBNHQR010000239.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7252)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7252)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7252
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1162258"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1422
                     /gene="ccoN"
                     /locus_tag="AC16VR_05530"
     CDS             <1..1422
                     /gene="ccoN"
                     /locus_tag="AC16VR_05530"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017845263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045277 - respiratory chain complex IV
                     [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HIR6010652.1"
                     /translation="QTYSDRVVTTLVLLAIAWSIVGMTAGVWAAAQLVWPTLDFGQSW
                     LSYGKVRTLHTNAVIFGFGVTALMGTAFYSVQRTSHVRLFAPGLAWFTCWAWQITVLI
                     GGISLLAGYNAGKEYAELEWPLDIAITVAWVCFAVVFFGTIARRRIKPIYVSNWFYGG
                     LIIVIAMLHIVNSLALPVTLTKSYSLYAGAQDAIVQWWYGHNAVGFLLTGGFLGMMYY
                     FLPKQAEQPIWSYRLSIVAFWAFTYSYIWAGPHHLHYNSIPEWVQSLGMVMSLVLLAP
                     SWATMVNGIMTVSGAYEKLRTDPAMKFIVLSLAFYGLATFEGPMMSIKSINVVSHFTE
                     WTIGHVHSGALGWNALITFGTFYFLVPRLMGSKLYSVKLANWHFWLALAGTMLYIVAM
                     WGAGVSQGLLWLNIDDMGELRYSFTDIMAATVPYYVIRLLAGAVFLVGALLMAYNLYR
                     TVSGKRTIEVHAPSADDAAGASA"
     gene            1419..2042
                     /gene="ccoO"
                     /locus_tag="AC16VR_05535"
     CDS             1419..2042
                     /gene="ccoO"
                     /locus_tag="AC16VR_05535"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020800672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HIR6010653.1"
                     /translation="MSGIGLHRKLEENSGLLIFGILFVSAIGGLVQVLPSLFQESLKT
                     PAANTRVYTPMELVGRDIYVREACSTCHSQQVRPLFAEVRRYGPYSRSGEFVYDRPFL
                     WGSKRTGPDLHRVGGKYSDAWHEIHMIDPRAVVPDSIMPAYPWLAERAANADGDIEAK
                     LRGLVTLGHPYTDEDIAGAAASLEGATELDALIAYLQMLGTGMEETP"
     gene            2039..2182
                     /locus_tag="AC16VR_05540"
     CDS             2039..2182
                     /locus_tag="AC16VR_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010654.1"
                     /translation="MSYFLFAGDMLVMAFMTILVVWVSLKSSGDQLERAARIPLEDER
                     HDG"
     gene            2175..3092
                     /gene="ccoP"
                     /locus_tag="AC16VR_05545"
     CDS             2175..3092
                     /gene="ccoP"
                     /locus_tag="AC16VR_05545"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HIR6010655.1"
                     /translation="MADMPSDFWSGWIITLTVASLAGLVWLIFSVYFGKDPDSDHTSP
                     VWDETLSEGANPAPMWWFWMILAALVFSAIYLMLYPGLGSHAGIMAWSQGGALEKSMN
                     RFDAAFAERRQGVVSAELADLHDDVDAMGTARGIFTRNCAACHGPQGLGQANAFPNLR
                     DTAWQWGGTPEAISQSITAGRRGAMVGWLPVLGEDGVEQLVTYVKTLSAGTSDPNAPA
                     ATQFTTYCSACHAADGTGVAMLGGPDLTDDVWLYGGSDDALRTSIAEGRNGIMPAFGD
                     RLDDVQLKLLVAWLTRPPKASARTEAAAR"
     gene            3129..4256
                     /locus_tag="AC16VR_05550"
     CDS             3129..4256
                     /locus_tag="AC16VR_05550"
                     /inference="COORDINATES: protein motif:HMM:NF024777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIR6010656.1"
                     /translation="MNETDRRAQIGLVAVIGLILWFGLNPFDFARINNVQWLDPGPGL
                     QFGDSGLAVSEQAFTWPSDTGPAAVTVEMWVRASRVSDTEPRAWLVLMDDSKLTPLGV
                     RQEGGDLVVWDAVTNPDGERWYNDFRVPYAMRPGIEQHIAITSHEDGPLIYIDGSSAG
                     TYAGYPIPLARKGEPFGGRLVVGAGPPWVTPWMGEVLGLALYDRALTATEVEQHANAG
                     PGSGFAELARNSGAVGAYQFDAGGGTSVQDQAGGGHDLKIPEYFRPPGRGLLRSVDPA
                     ERTALWNVGDAILNVLGFAPLGFLISIVLRKHVKPTSLILTATMVGFVVSLGIEWTQS
                     LMITRSSSLQDLVLNTVGSLVGAVAALASSRFRGRENNTKA"
     gene            complement(4299..4375)
                     /locus_tag="AC16VR_05555"
     tRNA            complement(4299..4375)
                     /locus_tag="AC16VR_05555"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4339..4341),aa:Met,seq:cat)
     gene            4460..5233
                     /locus_tag="AC16VR_05560"
     CDS             4460..5233
                     /locus_tag="AC16VR_05560"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6010657.1"
                     /translation="MDKPRTALITGASAGIGKAFADALAAGGVNLVLVARRADRLQQA
                     AQALREAHGIDVATISADLSDPTAPVRIHEETESRGLVIDYLINNAGVAGPDLLEPDT
                     WQANAGFHQLMMIAPAHLCHLYVPAMRARRWGRIVNVASVAARIPRAGGCNYGPAKAF
                     LVALSEELSLTLKAEGIKVCALCPGFTHTEFHNEGSAAELKANTPAFVWYEAETVVKE
                     GLRALERGRSVYVSGRMYRWMDPFFQSVWTRRLFKIAAS"
     gene            complement(5281..6372)
                     /gene="ychF"
                     /locus_tag="AC16VR_05565"
     CDS             complement(5281..6372)
                     /gene="ychF"
                     /locus_tag="AC16VR_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002552142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HIR6010658.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIDAENFPFCTIDPNTGVV
                     PVPDPRLERVAQLARSAKIVPTSMEFVDIAGLVAGASRGEGLGNQFLAHIRETDAIAH
                     VVRCFEDDNVTHVSGAVSPADDIEIINTELALADLETAERVFDRTRRIANAGDKDAKL
                     LVAVLDKVIGGLNTGRPVRALALGPEELKLLHECHFITAKPVLYVANVAEDGFDNNPL
                     LDGVRAIAAAESSEVVVICNKIEAEIVELEEDERAEFLEDLGIEEPGLNRVIRAGYRL
                     LGLHHYFTAGPKEARAWTVRIGTRAPQAAGVIHTDFEKGFIRAEVIAYDDYVGCGGEQ
                     GAKDTGKWRLEGKDYVVQDGDVILFRFNV"
     gene            complement(6373..6981)
                     /gene="pth"
                     /locus_tag="AC16VR_05570"
     CDS             complement(6373..6981)
                     /gene="pth"
                     /locus_tag="AC16VR_05570"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009617433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIR6010659.1"
                     /translation="MADVSLIVGLGNPGPRYELTRHNAGAGFVEYLANRFGVVLRDEA
                     RFRGRMGRGEVLGHDVRLLIPTTYMNLSGSSVAAVARFYKLAVDEILVAHDEMAFEPG
                     MLRLRTGGGDNGHNGLRSIISQLGNAAGFPRLRIGVGHPGDRGRVEAYLTTERMPESE
                     RGQMEAAWRMEDAVLAAILDGDLQAAMNALHAPAGAGDEGED"
     gene            complement(6984..>7252)
                     /locus_tag="AC16VR_05575"
     CDS             complement(6984..>7252)
                     /locus_tag="AC16VR_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010660.1"
                     /translation="GDLPEFIEVYMAQVDVNQSVHLTDLALPEGVSIVALMHGSDRDA
                     NVVSVQMPRGGLEEEEAAEAAEAAAEEGGEASDEGAGGEEASED"
BASE COUNT         1245 a         2386 c         2273 g         1348 t
ORIGIN      
        1 cagacctaca gcgaccgggt cgtcaccacg ctcgttctgc tcgccatcgc ttggtcgatc
       61 gtcgggatga ccgccggcgt atgggcggcc gcgcagctcg tgtggccgac actggacttc
      121 ggacagagct ggttgtcgta cggcaaggtg cgcaccctgc acaccaatgc cgtcatcttc
      181 ggtttcggcg tgacggcgct gatgggcact gcgttctaca gtgttcagcg cacgtcccac
      241 gttcgtctct ttgcaccggg tctggcgtgg ttcacctgct gggcctggca gatcaccgtg
      301 ctgatcggcg gcatttcgct gctcgcgggc tacaacgccg gtaaggagta cgccgagctg
      361 gagtggcctt tggacatcgc gatcaccgta gcgtgggtgt gcttcgcggt ggtcttcttc
      421 ggcaccatcg cgcgtcgcag gatcaagccc atttacgtct cgaactggtt ttacggcggc
      481 ctgatcatcg tcatcgccat gctgcacatc gtgaacagcc tggccctgcc ggtcaccctg
      541 accaagtcct actctctcta cgccggcgcc caggatgcca tcgtgcagtg gtggtacggc
      601 cacaacgccg tgggtttcct gctgaccggc gggttcctcg gcatgatgta ttacttcctg
      661 cccaagcagg cagagcagcc gatctggagc taccggctgt ccatcgtggc cttctgggcg
      721 ttcacgtatt cgtacatatg ggcgggccct catcaccttc actacaactc catccccgag
      781 tgggtgcagt ccctgggcat ggtgatgagc ctggtgctgc tggccccgtc ctgggcgacc
      841 atggtcaacg gcatcatgac cgtcagcggc gcctacgaga agctgcgcac cgatcccgcc
      901 atgaagttca tcgtgctctc cctggcgttc tacggcctgg ccaccttcga gggcccgatg
      961 atgtccatca agagcatcaa cgtggtcagt cacttcacgg agtggaccat cgggcacgtc
     1021 cactcgggcg ctctgggctg gaacgccctg atcactttcg gcaccttcta ctttctggtg
     1081 ccccggttga tgggcagcaa gctctacagc gtcaagctgg ccaactggca cttctggctg
     1141 gcgctggcag gcaccatgct ctacatcgtc gccatgtggg gcgccggcgt ctcccagggc
     1201 ctgctgtggc tgaacatcga tgacatgggc gagctgcgct acagcttcac cgacatcatg
     1261 gcggccaccg tgccctacta cgtcatccgc cttctggccg gggccgtatt cctcgtcggg
     1321 gcgctgctga tggcctacaa cctttaccgg accgtctccg gcaagcgcac gatcgaggtg
     1381 cacgcgccct cggccgacga cgccgcagga gcatccgcat gagcggtatc ggtctgcatc
     1441 gcaagctgga ggagaactcg ggtctgctca ttttcgggat tctgttcgtg tctgcgatcg
     1501 gcgggctggt tcaggtactg ccgagccttt ttcaggaatc cctcaaaacg cccgcggcaa
     1561 atacccgcgt ctacacgccc atggagctgg tcgggcggga catttacgtg cgcgaagcct
     1621 gcagcacctg ccactcccag caggtgcggc cgctctttgc cgaggtccgc cgctacgggc
     1681 cctacagccg ctcgggcgag ttcgtctacg accgcccgtt tctctggggt tccaagcgca
     1741 cggggcccga tcttcaccgg gtcggtggca agtacagcga cgcctggcac gagatccaca
     1801 tgatcgatcc ccgcgccgtg gtgcccgact ccatcatgcc cgcctaccca tggctcgcgg
     1861 aacgggcagc gaacgcggac ggcgacatcg aggcgaagct tcgcggcctg gtgacgctgg
     1921 ggcaccctta caccgatgag gacatcgccg gcgcggcggc gagcctcgag ggcgcgaccg
     1981 agctcgacgc gctgatcgcc tacctgcaga tgctcgggac cgggatggag gagacgccat
     2041 gagttacttt ctgttcgccg gcgacatgtt ggtcatggcg ttcatgacca tcctcgttgt
     2101 ctgggtatcg ctgaagagtt ccggcgatca gctggaacgc gccgcccgaa taccgctgga
     2161 ggacgagcgt cacgatggct gacatgccct cggatttctg gagcggatgg atcatcacgc
     2221 tgaccgtggc gagcctggcg gggctcgtct ggctcatctt cagcgtctat ttcggcaagg
     2281 atcccgattc ggaccacacc tcgccggtat gggacgaaac gctttcggag ggcgccaacc
     2341 cggcaccgat gtggtggttc tggatgattc tcgccgccct ggtcttttcc gccatttacc
     2401 tgatgctgta ccccggcctc ggcagccacg ccgggatcat ggcatggtcg cagggtggcg
     2461 ctctggagaa gagcatgaac cgcttcgatg cggccttcgc cgagcggcgc cagggcgtcg
     2521 tgagcgccga gctggcggat ctgcatgacg acgtcgatgc catgggcacg gcccggggca
     2581 tcttcacccg caactgcgcg gcctgccacg gtccccaggg actcggccag gccaatgcct
     2641 ttcccaacct ccgggacaca gcctggcaat gggggggcac gcccgaggcg atcagccagt
     2701 ccatcacggc cggccgccgc ggcgccatgg tcggctggct acccgtgctc ggcgaggacg
     2761 gcgtcgagca gctggtgact tacgtcaaaa ccctttcagc cggcacgagc gatccgaacg
     2821 cgccggcggc aacgcagttc acgacctact gcagcgcctg tcacgcagcc gacggaaccg
     2881 gcgtcgccat gctcggcggc ccggacctca ccgacgacgt gtggctgtac ggcggctcgg
     2941 acgacgcgct gcggacgtcc atcgccgagg gccgcaacgg catcatgccc gccttcggcg
     3001 atcgcctgga cgacgttcag ctgaagctgc tcgtcgcctg gttgacccga ccgcctaagg
     3061 cctcggcacg cacggaagcc gccgcccgct gatccgaatc gccagatgct ccaaggaatc
     3121 acccgagcat gaatgagaca gaccgtcgag cgcagatcgg gctcgttgcg gtcatcgggc
     3181 tgattctctg gttcggcctg aatcccttcg acttcgctcg aatcaacaat gtccagtggc
     3241 tcgatccggg gcccggcctg caattcgggg actcgggact cgctgtctcc gagcaagcct
     3301 tcacgtggcc gtcggatacc ggtcccgcgg cggtgaccgt tgaaatgtgg gtgcgcgcga
     3361 gccgcgtttc ggacaccgaa ccaagagcct ggctggtcct gatggacgac tccaagctca
     3421 ccccgctggg cgtacgtcag gaagggggag acctcgttgt ctgggacgcg gtgacgaatc
     3481 cggatggcga acgctggtac aacgatttcc gggttcctta cgccatgcgt ccgggtatcg
     3541 agcagcacat cgccatcacc tcccacgaag acgggccact catctacatc gatggttcga
     3601 gtgcaggcac ctacgcgggc taccccattc cgctagcgcg caaaggcgaa ccgttcggcg
     3661 gtcggctcgt ggttggcgcc gggccaccct gggtcacgcc gtggatggga gaagtgctcg
     3721 ggctcgccct atacgatcgc gcgctgaccg cgacggaggt agagcagcat gcgaacgcgg
     3781 gcccgggatc cggattcgcc gaactggcaa ggaactcggg tgccgttggc gcctatcagt
     3841 tcgacgcggg cggcggcacc tcggttcagg atcaggcggg cggcgggcac gacctcaaga
     3901 tccccgagta ctttcgccca ccgggccgcg ggctgcttcg gagcgtcgat ccggctgagc
     3961 gcacagcact ctggaacgtc ggcgacgcga ttctgaacgt tctgggcttt gccccattgg
     4021 gcttcttgat atcgattgtg ctacgcaagc acgtgaaacc gacttccctc attctgaccg
     4081 caacgatggt tggtttcgtc gtcagcctag gcatcgagtg gacacaatcg ctgatgatta
     4141 cgaggtcttc atctttacag gatctcgtgc tgaacaccgt tggcagcctg gtcggagccg
     4201 tggcggccct tgccagttcc cgttttcgag gccgcgaaaa caataccaaa gcgtaacaga
     4261 gccagatgac acggccggtt gttcgtcgag aaagggattg gtagctacgg ttggacttga
     4321 accaacgacc ccagcattat gaatgctgtg ctctaaccag ctgagctacg tagccatggc
     4381 ttgcctgtgc tggcgaagga tcgcgattgt cctgacaagc cgatgagctg tcaacgtgcg
     4441 gcggacgcag gaggacggcg tggacaaacc cagaacggcg ctgattaccg gagcatctgc
     4501 agggatcggc aaggccttcg ccgatgccct ggcagcgggg ggtgtcaatc tggtgttggt
     4561 ggcgcgacga gccgatcggc tgcagcaggc agcccaggcg ctgcgcgaag ctcacgggat
     4621 cgacgtcgcc acgatcagcg cagacctatc cgatccgacg gcccccgtgc gcatacacga
     4681 ggagacggag agccgtggcc tggtcatcga ctacctcatc aacaacgcgg gggtagccgg
     4741 gcccgacctc ctcgagccgg atacctggca ggccaacgcc ggcttccatc agctgatgat
     4801 gatcgcgccc gcgcacctgt gccatctgta cgtgccggcc atgcgtgccc ggcggtgggg
     4861 gaggatcgtc aacgttgcat cggtggcggc gcgcatacct cgcgccggcg ggtgcaacta
     4921 cggaccggca aaggcctttc tggtggcgct ctcagaggag ctctccctca ccctgaaggc
     4981 tgagggcatc aaggtgtgcg cgctgtgccc gggcttcacg cacaccgagt ttcacaacga
     5041 aggatccgca gcagagctca aggcgaacac gcccgccttc gtctggtacg aggcggagac
     5101 ggtggtgaag gaaggattac gggccctcga gcggggccgc agcgtctacg tgtccggtcg
     5161 catgtatcgc tggatggacc cgttctttca gtcggtgtgg acccgccgac tcttcaagat
     5221 cgcggcgagc tgaacgcccg acccggccgg tgcctcgaat cctgcgagcc gaccccgaga
     5281 tcagacattg aagcggaaga ggatcacgtc gccgtcctga acgacgtagt ccttgccctc
     5341 tagccgccac tttccggtgt ccttggcacc ctgctcgccg ccgcagccga cgtagtcgtc
     5401 gtaggcgatg acctccgcgc ggatgaatcc cttctcgaag tcggtatgaa tcacacctgc
     5461 cgcctggggc gcccgcgtgc cgatgcggac ggtccaggcg cgagcctcct tgggtccggc
     5521 ggtgaagtaa tggtgcagcc cgagcaggcg atagcctgcc cggatgacac gattgagtcc
     5581 cggctcttcg ataccgagat cctccagaaa ctccgctcgc tcgtcttctt cgagctcgac
     5641 aatctcggcc tcgatcttgt tgcagatgac caccacctcc gaggattcgg cagcggcgat
     5701 ggcccgcacg ccgtccagca acgggttgtt gtcgaagccg tcctccgcca cgtttgccac
     5761 gtagagaacg ggcttggccg ttatgaagtg gcactcgtgc agtaacttca gctcctcagg
     5821 accgagcgcc agcgcccgca ccgggcgtcc cgtattcagg cccccgatga ctttgtccag
     5881 cacggcgacc agcaacttgg cgtccttgtc gcccgcgttg gcgatgcgcc gggtccggtc
     5941 gaagacgcgc tccgccgtct ccagatcggc aagggcgagc tccgtattga tgatctcgat
     6001 gtcgtcagca ggcgataccg cgccggagac gtgggtgacg ttgtcgtcct cgaagcaacg
     6061 cacgacgtga gcaatggcat cggtttctcg aatgtgcgcg aggaactgat ttccgagccc
     6121 ttcaccacgg gacgcaccgg cgacaagacc ggcgatatcg acgaactcca tgctggttgg
     6181 aacgatcttg gccgaacggg ccagctgggc aacccgctcg agccgcggat cagggacggg
     6241 gaccacacca gtattgggat cgatggtgca gaacggaaag ttctccgcat cgatgcccgc
     6301 cttggtcagg gcgttgaaca gcgtggattt gccgacgttc ggcaacccga cgattccgca
     6361 cttgaatccc attcagtcct ccccttcgtc gcccgccccc gccggcgcat gcagggcgtt
     6421 catggcagcc tgcagatccc cgtcgagaat cgccgccagc accgcgtctt ccatacgcca
     6481 ggcggcttcc atctgacccc gttccgactc gggcatgcgc tcggtagtga ggtaggcctc
     6541 gacacggccc ctgtctccgg gatggcctac cccaatacgt agacggggaa aacccgcagc
     6601 gttgccgagc tgagagatga tgctgcgcaa accgttgtgc ccattgtcac caccgcccgt
     6661 cctcagacgc agcatgcccg gctcgaacgc catctcgtcg tgggcaacca gaatttcgtc
     6721 cacggccagc ttgtagaagc gcgccaccgc agccaccgag ctgccactca gattcatgta
     6781 ggtggtggga atgagcaggc gcacatcatg gccgaggacc tcaccgcggc ccattcggcc
     6841 cctgaaacgc gcctcgtccc gaagcacgac gccgaaccgg ttagccaggt actcgacgaa
     6901 tccggcaccc gcattgtggc gcgtgagctc gtaccgcggg cccgggtttc ccaggcccac
     6961 gatcaggctg acgtccgcca tggtcagtct tcgctggctt cctcgccgcc agcgccttcg
     7021 tcggaggctt cgccgccctc ttcagcggcc gcttccgcgg cctcggcggc ctcttcctct
     7081 tccagcccgc cgcgcggcat ctggacgctg accacgttgg catcacggtc gctgccgtgc
     7141 atcaaggcga cgatgctgac cccttccgga agcgccagat cggtgaggtg taccgactga
     7201 ttgacgtcca cctgagccat gtagacctcg atgaactcgg gcagatctcc ag
//
LOCUS       DBNHQR010000240         2891 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_784626, whole genome shotgun sequence.
ACCESSION   DBNHQR010000240 DBNHQR010000000
VERSION     DBNHQR010000240.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_784626"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="AC16VR_05580"
     CDS             <1..856
                     /locus_tag="AC16VR_05580"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6010661.1"
                     /translation="AKPSTGAGGGLGRAHALLLAQHGAAVVVNDLGGARDGSGEGHAM
                     ADQVGAEIEAAGGRAAADYGSVSDPAAAKQMVQIAVDRFGKIDICINNAGILRDKSFK
                     NMTDDMWDVVVDVHLRGTYLVAKAAWDQMIEQGTGGRIINTSSTSGLIGNFGQANYGA
                     AKAGIAGLTRVLALEGMKYGITVNTLAPAAWSRLTEDIMPEGMEERLAPEKVSPAVVW
                     LCTDDAANVTGRQFCAGQNRVSLLSWQVTEIADKNAMDAPWTVEEIGERMQATMDAWP
                     KLLKPMEV"
     gene            complement(864..1658)
                     /locus_tag="AC16VR_05585"
     CDS             complement(864..1658)
                     /locus_tag="AC16VR_05585"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HIR6010662.1"
                     /translation="MIVEQIWTGNSYRNFNYLIVCPESGEALAIDPLDHKKGLARARD
                     KGWQITQVLNTHEHPDHTGGNGAVIEATGAKLLAHANAQRSIKGMDVALNAGDVVKVG
                     RTVELLALDTPGHTMAHVCLLSQTETPALFCGDTLFNAGAGNCHGGGHPDELYQTFAS
                     QLDQLPETTLVYPGHDYITNNLAFTLDREPDNEAAAALLKELEANHDPDAALVTSMAQ
                     EKEINTFFRLTSRTVIDRLREAFPDLPDEPTPHEVFVKLRELRNSW"
     gene            complement(1713..2507)
                     /locus_tag="AC16VR_05590"
     CDS             complement(1713..2507)
                     /locus_tag="AC16VR_05590"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008173865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR6010663.1"
                     /translation="MKTQRLFLLIMVCALSACATSPMGRSQLILFPDSEMDQMGVAAY
                     REMQQQTPREQTAGVNDYVQCVVEHVVLAMPDGRMADWEVTVFADDQANAFALPGGKI
                     GVYTGLLDVAENQDQLATVIAHEVAHVLARHSNERVSTTYATQTGLQVVQIAGGTSSP
                     ARNQLYGLLGLGAQVGVLLPFSRAQESEADLMGLDLMAEAGFDPRESVRLWENMAESG
                     GARPPEFLSTHPSGETRMRDLNERMRRAMDLSLQARSAGRNPDCRR"
     gene            2678..>2891
                     /locus_tag="AC16VR_05595"
     CDS             2678..>2891
                     /locus_tag="AC16VR_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010664.1"
                     /translation="MTDIPDVGAQDPELKNSPIALESDEETVLLREEIPDEPFCYFNS
                     EAFPNGAVVKSGPMKLRCDLGIWVPIG"
BASE COUNT          513 a          923 c          966 g          489 t
ORIGIN      
        1 ggccaagccc tccaccggag ccggtggagg gcttggccgc gcccacgccc tgctgctggc
       61 gcagcacggc gctgcggtgg tggtcaacga tctgggcggc gccagggacg gctccggaga
      121 ggggcacgcc atggccgacc aggtgggggc cgagattgag gctgcgggcg gcagggccgc
      181 agccgactac gggtcggtga gcgatccggc cgcagcgaag cagatggttc agattgccgt
      241 ggaccgattc ggcaagatcg acatctgcat caacaacgcc ggcattttga gagacaaatc
      301 gttcaagaac atgaccgatg acatgtggga cgtcgtggtg gacgttcacc tgcgcgggac
      361 ctacctggtc gccaaggcgg cctgggacca gatgatcgag cagggaaccg gcggccggat
      421 catcaacacc agctccacct ccgggctcat cggcaacttc ggtcaggcca actacggcgc
      481 cgccaaggcg ggcatcgccg ggctcacccg ggtgctggcg ctggagggga tgaaatacgg
      541 cataaccgtc aacaccctgg ccccggccgc ctggtcacgc ctgaccgagg acatcatgcc
      601 cgaaggcatg gaggaacggc tggccccgga gaaagtctct ccggccgtgg tctggctgtg
      661 caccgacgac gcagccaacg ttaccggccg tcagttctgc gccgggcaga accgggtctc
      721 gctgctctcc tggcaggtga ccgagatcgc cgacaagaac gccatggacg cgccgtggac
      781 ggttgaggag atcggcgagc gcatgcaggc caccatggac gcctggccca agctgctcaa
      841 gcccatggag gtctaggccg cggctaccag gagttcctga gctcgcggag cttcacgaac
      901 acctcatgag gggtgggctc gtccggcagg tccggaaacg cctcccgcag gcggtcgatg
      961 accgtgcggc tcgtcagccg gaaaaaggtg tttatctcct tttcctgggc catggacgtc
     1021 acgagagcgg cgtccgggtc gtgattcgcc tccagctcct tcagaagcgc tgccgcggcc
     1081 tcgttgtcgg gctcccggtc cagggtgaag gccaggttgt tggtgatgta gtcatgtccc
     1141 ggatacacga gggtggtttc cggcagctga tcgagctgtg aggcgaacgt ctggtagagc
     1201 tcgtcgggat gccccccgcc atgacagttg ccggccccgg cgttgaacag ggtgtcgcca
     1261 cagaacaggg ccggcgtctc cgtctgggac agcaggcaga cgtgggccat tgtgtggccc
     1321 ggcgtatcca gcgcaagcag ctccacggtt ctacccacct tgaccacgtc gcccgcattg
     1381 agcgcgacgt ccatgccctt gatgctgcgc tgggcgttgg cgtgggccag cagctttgcc
     1441 ccggtggcct cgatcaccgc accgttgccg ccggtgtgat ccgggtgctc gtgggtattg
     1501 agaacctggg tgatctgcca ccccttgtcc cgcgcgcggg ccaggccttt cttgtggtcc
     1561 agagggtcga tggccagcgc ctcgcccgac tccggacaga cgatgaggta gttgaagttt
     1621 cggtaggagt taccggtcca gatctgctcg acgatcatgc gcgtgcccgt ttagtcgaaa
     1681 gcgtcagtgt aacccacgca cgcgcccggg acttaccgac gacagtcggg gttgcgcccc
     1741 gccgagcgtg cctgcaggga gaggtccatg gcgcggcgca tacgctcgtt gaggtcacgc
     1801 atgcgcgtct ccccggaggg gtgcgtcgag agaaactccg gcggccgggc gcccccggat
     1861 tccgccatgt tctcccagag cctcacgctc tcgcggggat caaagcctgc ctcggccatg
     1921 aggtcgagac ccatcaggtc cgcctcgctc tcctgggccc ggctgaacgg caggagcacg
     1981 ccgacctgag cgccgagccc tagaagcccg tacagctgat tacgcgcggg agagctcgtc
     2041 ccgccggcga tctgcacgac ctgcaggccc gtctgggtgg cgtaggtggt cgatacccgc
     2101 tcgttcgagt ggcgcgccag cacgtgagcc acttcgtgcg cgatcacggt ggcgagctgg
     2161 tcctggtttt cggccacatc gagcaagccg gtgtagacgc cgatcttgcc ccccggaagg
     2221 gcgaaggcgt tggcctgatc atcggcaaag acggtgacct cccagtcggc catcctgccg
     2281 tcgggcatgg ccagcaccac gtgctcgacc acgcactgaa cgtagtcgtt gacgccggcc
     2341 gtctgctcgc ggggcgtctg ctgctgcatc tcccggtagg cggctacccc catctggtcc
     2401 atttccgaat ccggaaacag gatgagctga ctgcgcccca tgggcgaggt cgcgcaggcg
     2461 gacagcgcgc aaaccatgat caggagaaag agtcgttgtg tcttcacggg atgatcccca
     2521 tgtggcccgc ggaaaggatg cgcgaactgc ccgagagaga caacacgcgc gccaccctct
     2581 ccgggcattg tagaatgtcc atctacgggg ttgaccggat gccattgcat tcgttccgtc
     2641 cgctagcatg actcgaaagc caacaggaca cattccaatg accgacatac cggacgtggg
     2701 cgctcaggac cccgagctca agaactcgcc aattgcgctg gagagcgacg aggaaaccgt
     2761 gctgctcaga gaagagatcc cggacgagcc cttctgctac ttcaattccg aagcatttcc
     2821 aaacggcgcc gtggtaaaga gcggcccgat gaagctgcgc tgcgatctcg ggatctgggt
     2881 gccgatcggc t
//
LOCUS       DBNHQR010000241         5101 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_466215, whole genome shotgun sequence.
ACCESSION   DBNHQR010000241 DBNHQR010000000
VERSION     DBNHQR010000241.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5101)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5101)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5101
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_466215"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            474..1709
                     /locus_tag="AC16VR_05600"
     CDS             474..1709
                     /locus_tag="AC16VR_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR6010665.1"
                     /translation="MLVALTMVGAHSAERIDEMLDVDDPALADVESRIRAREFDQPIT
                     WLETHIDTLESISHRYDPELVRPLVLLGDAELGKGNYGTALEHYGRAVHVSRVNDGLH
                     SASHVEIVYREAQAYKAMGNYEQANNREEYAYQVLKRAHGGMDEDLLPGVYKLARWYR
                     DTQNLFSARAMYQHALEILIANGKHDDLESIPAYEGIAWSYRLERFPPYFMSGGETRA
                     SEFSSAYEESISINNFPAGERALQQIIRIYRNTDSINPVVVSEAILELADWYLLFDKT
                     KRAYPLYEYVYDALEELEPGLAGTYFGEPKLLYFPVPQDPRPPDLQERGDARTGYVEL
                     AYEISDSGYPRSLKTVASEPPGLMDFRVRKSLRLSRYRPMIVEGQALATQDFTYRYEF
                     DYFEELEPAQEVVEEEQDE"
     gene            complement(1857..2063)
                     /locus_tag="AC16VR_05605"
     CDS             complement(1857..2063)
                     /locus_tag="AC16VR_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010666.1"
                     /translation="MLLPLVGGAPPGWLGEDWGLCLPGFDGELGWLGELLGWLGELLG
                     EEGGVDGLGMLGGFGGWGGVWLLD"
     gene            2179..2877
                     /locus_tag="AC16VR_05610"
     CDS             2179..2877
                     /locus_tag="AC16VR_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010667.1"
                     /translation="MPGSPEGPDSGEPGEAGPGEKEPGVAGTEEAGSGQEQPGASGAG
                     DPGEDSPGWVDGDEAGEGDWETSNQVPAQTAEMPPMPSGSGGGPAEQAEPGQSEGDGE
                     LDKALESFDGEIMSEREVIMARSNENAGKTGSSIPMPGSGGGSGSQTGGSEGVPGAEQ
                     GGSDVPGGPAGMPRASSAPPPPMASAGDIPDDIPDAKDDDIIARQLREAAMKETDPEL
                     KEKLWDEYRRYKGA"
     gene            2923..4119
                     /locus_tag="AC16VR_05615"
     CDS             2923..4119
                     /locus_tag="AC16VR_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010668.1"
                     /translation="MKLLAVALLAGLCAGCVQEVVRTVDMTPPEQLDRVQTEDELLDI
                     GVAILDPNVPESYDEQIKQLVQPEIRRAEANFIPYVAKNLLQSTGNWGAVRVVPRPTH
                     AVDVVVEGKILHSTGENLAVEMSVHDATGRHWFSRRYEALASKYAYEEAIPQSIDPFQ
                     AIYKQLADDMLDYRKGLSYDEVMVIRRTAEMKFAQDFAPDAFADHITTTSEGEFELKR
                     LPAENDPMLSRVQRVREREYLFIDTLDEYYESFHRDMFESYQSWRRATYDEAIAYKAL
                     RAQSRARTIGGATAIIAGVAAMSESSDPYVDAGGIVSVIGGAMTIKGAIEKRAEAQIH
                     AEVLQEVGVAAEAEIVPYTIELENQTVRLQGSVDEQYEELRRILRRIYFEDLGLPAPE
                     EQPEET"
     gene            4119..>5101
                     /locus_tag="AC16VR_05620"
     CDS             4119..>5101
                     /locus_tag="AC16VR_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010669.1"
                     /translation="MSEDFGEIRPKSVPLTHSAPERPSASTRPSLRPLAAGASVLLVM
                     ATAFVVFVVVPDLVQPLTLPGTPATDTAGATSDSPRPAARDEKLPPFQALMKEQARAR
                     AQEELARFVELQLDLEQTMQVGQWGQAEYDAAKRLATAGDEQFLGESFEAAIESYAKA
                     ATALAALIEDGKARFASAMRDGRTALERRDEKNAAGAFARALTISPDDQEAQQGARRA
                     ALLPEVNTAMREAKNHELAAEYRDALAVYERVRAMDPKTAGLEAGIRAARAGIQAETV
                     KGHLSSGFAALEAGRLDAARAAFGQALKLDPGNPVALGGLEQVDDASAGRRL"
BASE COUNT         1015 a         1655 c         1622 g          809 t
ORIGIN      
        1 ggtctacaac ggtactacta ctgtatgcta taatagtttt atatattttt tacgtactgc
       61 atgcatatac aaagtggtgt gtggccatct ctcgatcgct cgatcgatcg ttcttgctgc
      121 ttgctactta cttagtacta atatgatgcg cccacctcat tgcaaagggt tcgggacaga
      181 tcagtccggt ggcgtcgatt cggtgttgag cgtcaatcat cggcgggccg cgatggtagc
      241 ccaaaaaccc gtcggcgagc cagcgacccg ctgccaggct cccgccgcgc gccttgactt
      301 gggatatggc cgattacaca ctcgaacgct cgcgacagat cgcggtctaa acgccaggtt
      361 tcggtgctgt cgggctcaca agtcgtggaa tctgaagaca ttacggagtt gcgggaggtt
      421 cgtgagtaac ctctgacgcc cacctcatgc tcttgactag gttgacaaca ggattgctgg
      481 tagcgctgac catggtcggc gcgcactctg ccgagcgcat cgacgagatg ctcgatgtag
      541 acgaccctgc ccttgcggac gtcgaatcgc ggatcagggc acgcgagttc gaccagccca
      601 tcacgtggct ggaaacccac atcgacacgc ttgaaagtat cagtcaccgc tacgaccccg
      661 agctggtccg gccgctggtt ctgctgggtg acgcagagct gggtaaaggc aactacggca
      721 cggcgctgga gcactacggg cgggccgtcc acgtcagccg cgtcaacgac ggcctgcaca
      781 gcgcctctca cgtggagatc gtgtatcggg aagcccaggc ttacaaggcc atgggcaact
      841 acgagcaggc caacaaccgc gaggaatacg cctatcaggt tctcaagcgg gcccacggtg
      901 gaatggacga ggatctgctg cccggcgtgt acaagctggc acggtggtat cgggacacgc
      961 agaacctctt ctccgctcgc gccatgtatc agcacgccct ggagatcctg atcgcgaatg
     1021 gaaaacacga cgacctcgag tctatacccg cctatgaggg catcgcgtgg tcatatcgac
     1081 tcgaacgttt tccgccctac ttcatgtccg gcggtgaaac cagggccagc gagttctcct
     1141 ctgcctacga ggagtcgatc tccatcaaca atttccctgc cggcgaacgg gcgctgcagc
     1201 agataatccg gatttaccgg aacaccgaca gcattaaccc cgtcgtggtc agtgaggcca
     1261 ttctcgaact cgccgactgg tatctgctct tcgacaagac caaacgcgct tatccactct
     1321 acgagtacgt ttacgatgct ctggaggagc tggagcccgg tttggcgggg acctatttcg
     1381 gcgagcccaa gctgctctat ttcccggtac cgcaggatcc ccggccgccc gatctgcagg
     1441 agcgcggcga cgcccggacc ggctacgtcg agttagccta tgaaatctcg gacagcggct
     1501 acccccgctc gctcaagacg gtggcatccg agcccccggg cctgatggat ttccgcgtcc
     1561 gtaagagcct gcgcttgtcc agataccggc cgatgatcgt ggagggacag gctctggcga
     1621 cccaggactt cacgtatcgc tacgagttcg actatttcga ggagctcgag cctgcccagg
     1681 aggtcgttga ggaggagcag gatgagtaaa ccggctcgta cagccaccgt tcagcttcct
     1741 cgcggtaggc tccccgcgtc tgcgcagcgc tatgtcggaa aggtgagcat gtccagatcg
     1801 caacggcgtt cggcccgcac cctcgcgctg gccgtcgtgc ttgccttggc gggctgtcag
     1861 tccagcagcc agacgccgcc ccagccccca aagccaccca gcatgccgag cccgtctaca
     1921 ccaccctctt cgccgagcag ctcgccaagc cagcccagca gctcgccgag ccagccgagt
     1981 tccccatcga aacccggcaa acagagcccc cagtcctctc ccagccagcc aggcggcgct
     2041 ccgccgacca gcggcagcag caccccgccc ccgggaggtc agcccagagc cggcgaggca
     2101 gacattcctc cgcctcccga cagcactgcg ggcaccggcg aagataccgg caccgagcag
     2161 ctgcccggaa tgccgggaat gccgggatcc ccagaaggcc cggattccgg tgagcctggg
     2221 gaagccggac ccggcgaaaa ggagccgggc gtcgcgggca ccgaggaagc aggtagcgga
     2281 caggagcagc ctggcgcatc cggtgccggc gatcccggag aggattcgcc cggttgggtg
     2341 gatggcgacg aggcgggtga aggggattgg gagaccagca accaggtgcc agcgcagacg
     2401 gccgagatgc cgcccatgcc gtcaggttcc ggaggcggcc ccgcagagca ggccgaaccg
     2461 ggccaatccg agggtgacgg agagctcgac aaggcgctgg agagtttcga cggcgagatc
     2521 atgtcggagc gggaggtcat catggcacgc tccaacgaaa acgccggaaa aaccggctcg
     2581 tcgattccca tgccgggttc cgggggcggc agcggaagcc agaccggcgg gtcggaaggc
     2641 gttcccggcg cggagcaggg aggctccgac gtcccgggcg gccccgcggg tatgcccagg
     2701 gcgagctctg caccgccgcc tcccatggcc tcggcaggcg acatccccga cgacatcccc
     2761 gacgcgaagg acgacgacat cattgcacgc cagcttagag aggccgcgat gaaggagacg
     2821 gacccggagc tgaaggaaaa actatgggac gagtatcggc gctacaaggg cgcttgaacc
     2881 agcagtaggc aaatgcgtgt gcagccagac tcgaaagaaa gattgaagtt actcgcagtg
     2941 gcgttgctcg ccggtctctg cgccggctgc gttcaggagg tcgtccgaac cgtggacatg
     3001 acccctcccg agcagctcga tcgcgttcag accgaggacg agctgctcga catcggcgtg
     3061 gccattctcg acccgaacgt gccggagagc tacgacgagc agatcaagca gctcgttcag
     3121 ccggagatcc gccgcgcaga ggccaacttc attccctacg tcgcaaagaa cctgctgcag
     3181 agcacgggaa actggggggc cgtacgcgtc gtgccgcgcc cgacccacgc cgtcgacgtc
     3241 gtcgttgagg gcaagattct ccattccaca ggcgagaacc tggccgtgga gatgtcggtg
     3301 cacgatgcga ccggccgcca ctggttttcc cggcgctacg aagcgctggc cagcaaatac
     3361 gcctacgaag aggcgattcc gcagtccata gacccgtttc aggccatcta caagcagctg
     3421 gccgacgaca tgctcgacta tcgcaagggc ctgagctacg acgaggtcat ggtgatccgc
     3481 cgcaccgcgg agatgaagtt cgcgcaggat ttcgcgccgg acgcgttcgc cgaccacatc
     3541 accaccacct ccgagggaga gttcgagctc aaacgtcttc ccgctgagaa cgaccccatg
     3601 ctgagccgcg tccaacgcgt tcgggagcgc gagtacctct tcatagacac gctggacgag
     3661 tactacgaga gtttccaccg cgacatgttc gagtcctacc agagctggcg ccgagcgacc
     3721 tacgacgaag ccatcgccta caaggcgctg agggcgcagt cgcgcgctcg gaccatcggc
     3781 ggcgccacgg ccatcattgc cggggtcgcc gccatgagcg aaagcagcga cccatacgtg
     3841 gacgccggcg gcatcgtgag cgtcattggc ggcgccatga ccatcaaagg tgccatcgag
     3901 aaacgcgccg aggcgcagat tcacgcggag gtgctgcagg aagtcggtgt cgccgcggaa
     3961 gctgagatcg tcccctacac catcgagctg gagaaccaga ccgtgaggct ccaggggtcg
     4021 gtggacgagc agtacgaaga gctgcgtagg attctccgcc gcatttactt tgaggatctg
     4081 ggtctacctg ctcccgaaga acaaccagaa gagacctgat gagcgaagat ttcggcgaga
     4141 tccggcctaa atcggtcccg ctcacgcaca gcgctccgga acggccgtcg gcctccacgc
     4201 gcccctcgct caggcccctg gcagcgggcg cctccgtgct gctcgtcatg gcgacagcct
     4261 tcgtcgtctt cgtggtcgtc cccgatctgg tccaacccct gacgctcccc ggcacgccgg
     4321 ccaccgatac cgccggcgcg acctcggact cgccacgccc ggcggccagg gacgagaagc
     4381 tgcctccatt tcaggcgctg atgaaggagc aggcgcgggc cagggctcag gaggagctgg
     4441 cacggttcgt ggagctccag cttgacctgg agcagaccat gcaggtcggg caatggggac
     4501 aggccgaata cgatgccgcc aagcgcctcg ccaccgccgg cgacgagcag tttctcgggg
     4561 agtcgttcga ggccgccatc gaaagctacg cgaaggcggc aactgcgctg gccgccctca
     4621 tcgaggacgg caaggcgcgt ttcgcaagcg cgatgcggga cggtcgcacg gccctggaac
     4681 gacgcgacga gaaaaatgct gcaggggcgt tcgcccgggc gttgaccatc tctcccgacg
     4741 atcaggaagc tcagcaaggc gcgcggcgag ccgcgctgct acccgaggtg aacacggcga
     4801 tgcgggaagc caagaatcac gagctggccg cggagtatcg ggacgccctc gccgtctacg
     4861 agcgagtgcg cgcaatggac ccgaagaccg cgggcctcga agcgggcatc cgggctgccc
     4921 gcgccggcat ccaggcggag acggtcaagg gccatctctc ctccggtttc gcggccctgg
     4981 aggccggcag gctcgatgcc gcgcgcgctg cctttggcca ggcactgaag ctggatcccg
     5041 gcaacccggt ggcgctcgga ggcctcgaac aggtcgacga cgcctctgcc ggccggcgtc
     5101 t
//
LOCUS       DBNHQR010000242         6273 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_593787, whole genome shotgun sequence.
ACCESSION   DBNHQR010000242 DBNHQR010000000
VERSION     DBNHQR010000242.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6273)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6273)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6273
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_593787"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1418)
                     /locus_tag="AC16VR_05625"
     CDS             complement(<1..1418)
                     /locus_tag="AC16VR_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HIR6010670.1"
                     /translation="MLTLEGPPARSAFRLNKLSGQLGLPEGALYAQYVHLLAVHQPLA
                     GQALERAQALLRYGPETGLPAPTGDLVATVVPRAGTISPWSSKATDIFAICGLDQVTR
                     VERGVRWFADRGLSDVQLALLYDRMTERAVGEADFPSVFANLPPKPMSTVALQAGGLA
                     ALEEANRALGLALSADEIEYLASAYLELGRDPNDVELMMFAQANSEHCRHKIFNASWS
                     IAGEPQPLSLFEMIRNTYRSINGAGILSAYSDNAAVIEGHRIERFGVDSASHEYGYDE
                     AAVHVLMKVETHNHPTAIAPYPGAATGSGGEIRDEGAVGRGSKPKAGLTGFTTSHLNI
                     PGYGQPWELETGKPEHMVSALDIMVEGPVGAAAFNNEFGRPALAGYFRTFEQTVAGDP
                     RQVRGYHKPVMIAGGVGSIRPEHVQPTAFPAATALIVLGGPAMLIGLGGGAASSMGSG
                     ESSSDLDFASVQRDNAEIERRC"
     gene            complement(1446..1892)
                     /gene="tadA"
                     /locus_tag="AC16VR_05630"
     CDS             complement(1446..1892)
                     /gene="tadA"
                     /locus_tag="AC16VR_05630"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017131735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HIR6010671.1"
                     /translation="MSHVDWMRLALEEAGRAAAAGEVPVGAVVVRDGVLLGCGFNCPI
                     SSSDPTAHAEIVALRAAAKRSGNYRLAGAAMYVTVEPCTMCAGALVHARIATLVYGAL
                     EPRSGAVTSTATVLENPLLNHRVEVVGGVLARECAEVLRAFFAERR"
     gene            complement(1917..3494)
                     /gene="guaA"
                     /locus_tag="AC16VR_05635"
     CDS             complement(1917..3494)
                     /gene="guaA"
                     /locus_tag="AC16VR_05635"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIR6010672.1"
                     /translation="MADKYDIHDQRLLIIDFGAQYTQLIARRVREAGVYCEIHPFDVP
                     EDFIREFAPRGVILSGGPESVTFADTPRIPSIVFELEVPILGICYGMQAMAEQLGGSV
                     EASDVHEFGHAEIEIVETAELLGTVRDTAGGHLPVWMSHGDRVVRTPPGFEVTASTPS
                     APVAAMEHREKRMYGVQFHPEVTHTQRGEEMLAHFARTLCRCDGLWTAANIVEDAIAH
                     ARDLVGDERVVLGLSGGVDSSVVAALLSRAIGDQLTCVFVDNGLLRKGEREEVERAFA
                     PTNALALNLVSVPAAEEFLGRLAGVVDPEAKRKVIGNTFIEVFDREAHKISGVSWLAQ
                     GTIYPDVIESAAGKYGKAHVIKSHHNVGGLPERMHLKLVEPIRELFKDEVRKIGMHLG
                     LPRELVYRHPFPGPGLGVRVLGEVTAARADVLREADAIYLEELRAAGMYDAVSQAFAV
                     LLPVKSVGVVGDARRYADVIALRAVETTDFMTARCARLPYELIERVSNRIINEIPHVS
                     RVVYDVSSKPPATIEWE"
     gene            complement(3495..4964)
                     /gene="guaB"
                     /locus_tag="AC16VR_05640"
     CDS             complement(3495..4964)
                     /gene="guaB"
                     /locus_tag="AC16VR_05640"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014871630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HIR6010673.1"
                     /translation="MLRIAHDALTFDDVLLLPAYSQCLPSEVSLATKLTRDIHLNIPL
                     MSAAMDTVTEARLAIAIAQEGGVGIVHKNMDIESQAREVRAVKKHESGIIRDPITIHP
                     DKTVADLIELTMEHKISGVPVVDGRRLVGIVTGRDVRFERRMDAKVRDVMTTKEHLVT
                     ADEEASFEKITDLLHKHRIEKVLLINDRFELKGMVTVKDITKAQAYPNACKDHRGQLR
                     VGASVGTSPDTDDRVAALVEAGVDVLVVDTAHGHSQRVLDRISWIKQRYPDVNVIGGN
                     VATADGAKALLSAGVDAVKVGIGPGSICTTRMVTGIGVPQITAIAEAVAVADSEGVPV
                     IADGGVRYSGDIAKAIAAGASVVMVGGLLAGTDEAPGEVELYQGRTYKSYRGMGSLGA
                     MTESNGSSDRYFQEAEGDHRKLVPEGIEGRVPYRGPVGPIVHQLMGGLRASMGYTGSN
                     DIETMRTQPRFVRVSAASMVESHVHDVAITKEAPNYPVS"
     gene            5023..>6273
                     /gene="xseA"
                     /locus_tag="AC16VR_05645"
     CDS             5023..>6273
                     /gene="xseA"
                     /locus_tag="AC16VR_05645"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HIR6010674.1"
                     /translation="MLASQADRPVYSVSELAHEARILIEERFDLVWVEGEISNFRRPA
                     SGHWYFTLKDRSAQVRCAMFAGRNRQVRFAPEDGMQVLIRGRVSLYEPRGDFQVIVEH
                     AEPAGEGALRQAFEALKTKLAEEGLFAIERKRPLPALPRHLAIVSSASGAALQDVLHV
                     IERRFPALTVTLLPVSVQGNEAEAQIRRALARVDQVGADVVLITRGGGSLEDLWAFNL
                     ESVARAVAACPIPTVVAVGHQTDFVIAEFVADLRAPTPSAAAELITPDAAELAGRVGL
                     LQQRLVRGLSADVRLRQALVGRLRAQLVDPRQMVTRSMQRADDLEERLRLALGNRLER
                     NRNRLWALQRSLRAFRPEPLLRQQAARVTATRHALGKSLQRALAGSAERLGSAARTLD
                     AVSPLHTLARGYAVLAEPAADGLGR"
BASE COUNT         1056 a         2171 c         1981 g         1065 t
ORIGIN      
        1 tggcagcggc gctcgatctc cgcgttgtcc cgctgaaccg aggcaaaatc gaggtcggac
       61 gagctctctc ccgatcccat gctcgaggcg gcgcccccgc cgagcccgat gagcatggcc
      121 gggccaccga gcacgatcag cgcggtcgcc gccgggaaag cggtgggttg gacgtgctcc
      181 gggcggatgc tgccgacgcc gccggcgatc atgacgggct tgtgatagcc gcgaacctgg
      241 cgggggtcgc cggcaacggt ctgctcgaaa gtacggaaat agccggccag agccggtctt
      301 ccgaactcgt tgttgaaggc ggccgcaccc acggggcctt ccaccatgat gtccagcgcg
      361 ctcaccatgt gttccggttt gccggtctcg agctcccagg gctggccata gccgggaatg
      421 ttgaggtgcg aggtcgtgaa gcccgtaagg cccgccttcg gcttcgaccc tcggcccacg
      481 gcgccctcat cgcggatctc gccgccggac ccggtggcgg cccccgggta cggggcgatg
      541 gccgtcgggt gattgtgggt ctctaccttc atcaacacgt ggacggctgc ttcgtcgtag
      601 ccgtactcgt gagacgcgga gtccacaccg aaacgctcga tgcgatgccc ctcgatgacg
      661 gctgcgttgt cggaataggc gctgagaatc ccggccccgt tgatcgaccg gtaggtattc
      721 ctgatcatct cgaacagcga aagcggctga ggttcgccgg cgatgctcca cgacgcgttg
      781 aatatcttgt ggcggcagtg ctccgagttc gcctgagcga acatcatcaa ttcgacgtcg
      841 ttcgggtcac gcccgagctc cagataggcg ctcgcgaggt attcgatctc atccgcggac
      901 agcgcaaggc ccaacgcccg gttggcctct tccagcgccg ccaggccgcc cgcctgcaga
      961 gcaaccgtgc tcatgggctt tggcggcagg ttcgcgaaaa ccgaggggaa gtccgcctcg
     1021 ccaaccgccc gctcggtcat gcggtcgtaa agcagcgcaa gctggacatc gctcaggccc
     1081 ctgtccgcaa accaccgcac gccccgttcg acccgggtca cctggtccag accgcagatg
     1141 gcgaagatgt cggtcgcctt gctggaccac ggcgagatcg tgccggcccg aggaaccacg
     1201 gttgcaacca gatcacccgt cggcgccggc agccccgttt ccggaccgta gcgaagcagc
     1261 gcctgcgcgc gctccagcgc ctgtcccgca agcggctgat ggacggccag gagatggacg
     1321 tactgagcgt acagcgcacc ttccggcagg cccagctgcc cgctcagctt gttcaggcga
     1381 aaggcggagc gggccggcgg cccttcgagc gtcaaaatct ccatgaaccg atcctcatcc
     1441 gatgctcacc gccgctcggc aaagaacgcg cgcaaaacct ccgcgcactc gcgcgcaagc
     1501 acgccgccca ccacctcgac ccggtgattc agcaacgggt tttccagaac ggtcgccgtg
     1561 ctggtcaccg caccggatcg cggctccaac gcgccgtaga ccagggtcgc gatccgcgcg
     1621 tggacgaggg ctccggcaca catggtgcac ggttccaccg tcacgtacat cgcagcaccc
     1681 gcaagccggt agttgccgga ccgtttagcg gcagcacgca gcgcaacgat ctccgcatgt
     1741 gccgtggggt cgctgctgct gatgggacag ttgaagccac accccagcag caccccgtcc
     1801 cgaaccacca ccgcgcccac gggcacctct cccgcggcgg ctgcgcgacc cgcctcttct
     1861 agagccaggc gcatccagtc aacgtggctc atcgccctcc cgtgccaacc acacggtcac
     1921 tcccactcga tggtcgctgg cggtttgctg gagacgtcgt aaaccacccg cgaaacgtgg
     1981 gggatctcat tgatgatccg gttggataca cgctcgatca gctcgtaggg caaccgtgca
     2041 cagcgggcgg tcatgaagtc cgtggtctcc accgccctca gggcgatgac gtccgcgtaa
     2101 cgccgtgcgt cgcctaccac gcccaccgac ttcaccggca gcagcacggc aaacgcctgg
     2161 ctgacggcgt cgtacattcc cgcagctcgg agctcttcga ggtagatggc gtccgcttcg
     2221 cgcaggacgt cggcacgggc ggcggtcacc tcgccgagca cccgcacgcc caggcccgga
     2281 ccgggaaacg ggtgccggta gaccagctcc cgaggcagcc ccagatgcat cccgatcttg
     2341 cggacctcgt ccttgaacag ctcgcgaatc ggctccacca gcttcagatg catccgttcg
     2401 gggaggccgc cgacgttgtg atgggacttg atgacatgag ccttgccgta cttgcccgct
     2461 gccgactcga tgacgtcggg ataaatggtg ccctgagcca gccagctcac cccggagatc
     2521 ttgtgcgctt cacgatcgaa gacctcgatg aacgtattac cgatgacctt gcgtttcgcc
     2581 tcggggtcca cgacaccggc caggcgcccc agaaactctt ccgcggccgg cacgctgacc
     2641 aggttgagcg ccagagcgtt ggtcggcgca aaagcacgct ccacctcttc ccgctcgccc
     2701 ttgcgaagca ggccgttgtc cacgaacacg cacgtcagct gatccccgat ggcccgggag
     2761 agcagagcag caaccacgct ggaatccacg cctccggaca accccagcac gaccctttcg
     2821 tctccaacca ggtcgcgggc gtgggcgatg gcgtcctcga cgatgttggc ggccgtccac
     2881 aggccatcgc agcgacacag cgtccgcgcg aagtgggcaa gcatctcttc gccccgctgg
     2941 gtgtgcgtca cctcgggatg aaactgcacg ccgtacatgc gcttttcgcg atgctccatc
     3001 gcagccacgg gcgccgacgg ggtgctggcg gtgacctcga agcccggagg cgtcctcacc
     3061 acccggtcgc cgtggctcat ccagaccggc agatgcccgc cggcggtgtc acgcaccgtg
     3121 ccgagcagct cggccgtctc gacaatttcg atttctgcgt ggccgaactc atggacgtcg
     3181 gacgcttcca cggaaccgcc cagctgctct gccatggcct gcatgccgta acagatgccg
     3241 aggatgggca cctccagctc gaacacgatg gacgggatgc gcggcgtgtc cgcaaacgtt
     3301 accgactccg ggccgccaga gaggatcacc ccccgcggcg cgaactcgcg tatgaaatcc
     3361 tccgggacgt cgaaggggtg aatctcgcaa taaacgccgg cctcgcgcac ccggcgggca
     3421 atcagctggg tgtactgggc accgaaatcg atgatcagga ggcgctgatc atgtatgtcg
     3481 tatttgtccg ccattcagct gacgggatag ttcggcgctt ctttggtgat cgccacgtcg
     3541 tgaacgtgac tctccaccat gctggccgcg ctcactcgca cgaagcgagg ctgggttcgc
     3601 atggtctcga tatcgttcga gccggtatag cccatcgacg ccctgagccc gcccatgagc
     3661 tgatgaacga tggggcccac aggcccacgg taaggcacgc gcccttcgat cccctcggga
     3721 acgagcttgc gatgatcccc ctctgcttcc tggaagtacc gatcgctgga cccgttggac
     3781 tcggtcatcg cacccagcga gcccatgccg cgataggact tgtaggttct gccctgatag
     3841 agctctacct ctccgggcgc ttcatcggtg ccggcgagca gcccgccgac catcaccacg
     3901 ctggccccgg ccgcgatggc tttggcaatg tcgcccgagt aacggacacc tccatcggca
     3961 atgacgggca ccccttccga gtcggcaacc gccaccgctt cggcgatggc cgtgatctgc
     4021 ggaacaccaa tgccggtaac cattcgcgtg gtgcagatgg agccggggcc gatgccgacc
     4081 ttgaccgcat ccacgccggc ggaaagaagc gctttcgcgc cgtccgcggt agccacgttg
     4141 ccgccgatga cgttgacgtc cggatagcgc tgcttgatcc agctgatgcg gtccagcacc
     4201 cgctgggagt gcccgtgagc cgtatccacc accagcacgt cgacgccggc ttccacgagg
     4261 gccgcgaccc ggtcgtcggt gtcggggctg gtacccacgc tcgcaccgac ccggagctgt
     4321 ccccggtggt ctttgcaggc attgggataa gcctgggcct tggtgatgtc cttgacggtc
     4381 accatgcctt tgagctcgaa gcgatcgttg atgaggagga ctttctcgat gcgatgcttg
     4441 tgaaggagat cggtgatctt ctcgaagctt gcctcctcgt ccgcggtgac gaggtgctcc
     4501 tttgtggtca tcacgtcgcg gaccttggcg tccatgcgac gctcgaaccg cacgtcgcgt
     4561 cccgtgacga tgccgaccag ccggcgtcca tccaccaccg gcacgccgga gatcttgtgt
     4621 tccatggtca gctcgatcag atccgcgacc gttttgtccg gatggatggt gatcgggtca
     4681 cggatgatgc cactttcgtg cttcttgacc gcccggactt cacgggcctg gctctcgatg
     4741 tccatgttct tgtgcacgat gccgaccccg ccctcctgcg cgatcgcaat agcaagccga
     4801 gcctccgtca ccgtgtccat ggcagcggac atgagcggga tattgaggtg gatgtctctg
     4861 gtcagcttcg tcgcgagcga aacttctgaa ggcaagcact gggagtaggc gggaaggagc
     4921 agaacgtcgt cgaacgtgag cgcgtcatgc gcaatacgca acattggtca ctccgcaaaa
     4981 gcggcattct atcggcgcac cccgatcatg taaactcgac ccatgctcgc cagccaagca
     5041 gatcgccccg tctacagcgt cagcgaactc gcccacgagg cgagaatcct catcgaggag
     5101 cgcttcgacc tggtctgggt ggagggcgag atctccaact tccggcgtcc cgcatccggc
     5161 cactggtact ttacgctcaa ggatcgctcc gcccaggttc gctgcgcgat gttcgccggc
     5221 cgcaaccgcc aggtacgctt cgctcccgag gacggcatgc aggtcctgat caggggccgt
     5281 gtcagccttt acgagccgcg cggcgacttc caggtcatcg tcgagcacgc ggagcccgcc
     5341 ggcgaaggcg cgctgcgcca ggccttcgag gccctgaaga ccaagctcgc agaggaaggt
     5401 ctcttcgcga ttgagcgtaa gcggccgctg cccgcgctgc cccgccacct ggcaatcgtc
     5461 tcgtcggcct ccggcgcggc tctccaggac gtcctccacg tcatcgagcg gcgctttccg
     5521 gcgctgaccg tcacgctgct gccggtcagc gtgcagggga acgaggccga ggcccagatc
     5581 cggcgcgcgc tggcgcgggt cgatcaggtc ggcgccgacg tggtgctgat cacgcgcggc
     5641 ggagggtctt tggaggatct ctgggcgttc aacctcgaaa gcgtcgcccg cgcggtggca
     5701 gcgtgcccga tacccacggt ggtggccgtg ggccaccaga ccgacttcgt catcgccgag
     5761 tttgtggccg acctgcgcgc acccacgcca tccgccgccg ccgagctcat cacgccggat
     5821 gccgcagagc tggccgggcg cgttgggctg cttcaacagc gactggtccg cgggctttcc
     5881 gccgacgtgc gcttgcggca ggcgctggtg gggcggctgc gagcccagct cgtcgatccg
     5941 cgccagatgg tgacccgcag catgcagcgt gcggacgatc tggaggagcg cctgcggctg
     6001 gccctcggca accggctgga gcggaaccga aaccggctgt gggcattgca gcgcagcctc
     6061 cgggccttcc gtccggagcc cctgttgcgc cagcaagcgg ccagggtcac cgccacgcgt
     6121 cacgccctgg ggaaatccct tcaacgcgcc ctggcgggct ccgcggagcg cctcggatcg
     6181 gccgcgcgca cgctggatgc cgtcagtcct ctgcacacgc tggcgcgtgg ctatgccgtg
     6241 ctcgccgagc ccgcagccga tgggctcgga cgg
//
LOCUS       DBNHQR010000243         2484 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_530390, whole genome shotgun sequence.
ACCESSION   DBNHQR010000243 DBNHQR010000000
VERSION     DBNHQR010000243.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_530390"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="AC16VR_05650"
     CDS             complement(<1..233)
                     /locus_tag="AC16VR_05650"
                     /inference="COORDINATES: protein motif:HMM:NF039725.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6316 family protein"
                     /protein_id="HIR6010675.1"
                     /translation="MLPEWFRSDEIFSDEGRWYFGARGGLHVGPYGDRELASARSLSV
                     SRRLRALRTDADRLRYVRKVLHEEWESLGVSASA"
     gene            complement(393..1166)
                     /locus_tag="AC16VR_05655"
     CDS             complement(393..1166)
                     /locus_tag="AC16VR_05655"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HIR6010676.1"
                     /translation="MKIATWNVNGLNARQEFVGLWLAHRQPDLVGLQEIKLVDEAFPA
                     DFFAGLGYQVLTHGQKAWNGVAVLSRLPVEIVQKGLPGQESAGSRLITVRVDDRLEFT
                     TVYCPNGKTLDHEDYAKKLAWFTSLSEYWRDRPSAGAVLCGDFNVVPEAVDSWRGEAG
                     DGRIFHTLEERAAFRSLLDAGLEDLFRMAKPDEQAFSWWDYRGGSFHRGHGLRIDLIL
                     GSRDVAERVVDVRTDRDYRKKKDGLTASDHAPVIAEIDL"
     gene            1251..2435
                     /locus_tag="AC16VR_05660"
     CDS             1251..2435
                     /locus_tag="AC16VR_05660"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6010677.1"
                     /translation="MSELSEFRNQTRAWLETSCPAGARGPGQIPWGSSKIPLESDVRL
                     WLERMAERGWTVPTWPKEYGGAELSRDQYMVLIDELKRINARTPLTGRGVNYIGPTIL
                     EFGTHEQKERWLPGIARGEGGWAMGYSEPGAGSDLASLSTRAELQGDQYVLNGRKTWT
                     SDAMDCDYIFVLARTDPDRPKHQGISLLLVDMNQPGVQVRPIRLLSGASPFNETTFEN
                     AVARADDVIGEVNAGWTVGKRLLQFERSTHAGINISGSQGGRSAESNLAERLKQFVPS
                     RDGKLQDPELRERLVRYEQNGHAQRLTQKRVIEELKARAPGYASSAVKLTGALHTQEG
                     DELLLAAMGTQGIVWDGPGLERSDSEALRTWLRQKSLTIAGGTKEVQLNIIAKRVLGL
                     PD"
BASE COUNT          482 a          808 c          790 g          404 t
ORIGIN      
        1 gcgctggcgg atacccccag gctctcccac tcctcgtgga gcaccttgcg gacgtacctg
       61 agccgatcgg cgtcggttct gagcgcccgt aagcggcgcg agacggagag gctgcgggcg
      121 ctggcgagct cgcggtcccc ataaggaccg acgtgcaacc cgccccgggc accgaagtac
      181 caccgcccct cgtcggagaa gatctcgtct gatctgaacc actctggaag cattccgtgc
      241 atccgccaat aaatctgccg gtaagcctga tcccaggtct gcctgctggg agacgatatc
      301 ccctttcggg ccctcaaacc gtgaaccatt taaccaagcg gtgtccagcc tgcggcctct
      361 gggccagtcc cgaaagcggg gcgaccaatc ttttaaaggt cgatctcggc gatgacgggg
      421 gcgtgatccg aggcggtcag tccgtccttc ttcttgcgat agtcccgatc ggtacggacg
      481 tcgaccacgc gctcggccac gtcgcgcgag cccagtatca ggtcgattct cagcccgtgg
      541 ccgcggtgaa acgacccgcc gcggtagtcc caccacgaaa acgcctgctc gtccggcttg
      601 gccatacgaa acaggtcctc aagccccgca tcgagcagcg aacggaaggc cgcacgctct
      661 tcgagcgtgt gaaaaatcct gccgtcgccg gcttcgcccc gccagctgtc gacggcttcc
      721 ggcaccacgt tgaagtctcc gcagaggacg gcccctgccg aggggcgatc acgccagtac
      781 tcgctgagcg aggtgaacca ggcaagcttc ttcgcgtagt cctcgtggtc gagcgtcttg
      841 ccgttggggc agtagacggt cgtgaactcc aggcgatcgt cgacccgcac ggtgatcagg
      901 cgggatcctg ccgactcctg gcctggcagt cccttctgga cgatttccac gggcaggcgg
      961 ctgagcaccg cgacgccgtt ccatgccttc tggccatggg tgaggacctg ataacccagc
     1021 ccggcgaaga aatcggcggg gaaggcttcg tcgaccagct tgatctcctg cagcccgacc
     1081 aggtcgggct gccggtgggc cagccacaaa ccgacgaact cctgtcgggc gttcagcccg
     1141 ttgacattcc aggtggcaat tttcatggat gaattgtagc gttaatcggt cgccgtgatc
     1201 gccgtttcaa ccggcgtgca tcgctggatc gagcgcttga gagaaaccag atgagcgagc
     1261 tgagcgagtt ccgcaaccag acgcgggcat ggctggaaac cagctgcccc gccggcgccc
     1321 gcgggccggg ccagataccg tggggcagca gcaagatccc gctggaaagc gacgtgcgcc
     1381 tgtggcttga gcgcatggcg gaacgcggct ggacggttcc gacctggccg aaggagtacg
     1441 gtggcgccga gctgagcagg gatcagtaca tggtgctgat cgatgagctc aaacgcatca
     1501 acgcccgcac gccgctgacc ggacggggcg tgaactacat cggtccgacc atactcgaat
     1561 tcggtacgca cgagcagaaa gaacgctggc tgccgggaat cgccagggga gaaggcggat
     1621 gggccatggg gtactccgag cccggagccg gatccgacct ggccagcctg agcacccggg
     1681 cggagcttca gggcgatcag tatgttctca acggccgcaa gacgtggaca tccgacgcca
     1741 tggactgcga ctacatcttc gttctcgccc gcacggatcc cgaccgcccg aaacaccagg
     1801 gaatcagtct ccttctcgtg gacatgaacc agcccggcgt gcaggtccgc cccattcgcc
     1861 tgctttcggg cgcctcaccc ttcaacgaga ccacgttcga gaacgccgtg gccagggccg
     1921 acgacgtcat tggcgaggtg aacgccggct ggacagtggg gaaacgtctg ctgcagttcg
     1981 aacgttccac ccacgcaggt atcaacatct ccggctccca gggcggccgc agcgccgaaa
     2041 gcaatctggc agaacgcctc aagcagttcg ttcccagtcg ggacggcaag ctgcaggatc
     2101 ccgagctgcg cgagcggctg gttcgctacg agcagaacgg ccacgcccag cgcctgaccc
     2161 agaagcgcgt catcgaggag ctaaaagcgc gcgcgccggg gtatgcctct tccgctgtga
     2221 agctgacggg cgcactgcac acccaggaag gggacgagct cctgctcgcc gccatgggga
     2281 cccaggggat cgtctgggat gggccaggac tcgaacgaag cgatagcgag gcactgcgca
     2341 cctggctgcg ccagaaatcc ctgaccattg ccggcgggac caaagaagtg cagctgaaca
     2401 tcatcgcgaa acgcgttctc ggcctgccgg attgacctcg aggagtttgc gagatgtcct
     2461 tcagctttac cgacgagcag gagc
//
LOCUS       DBNHQR010000244         2090 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_403506, whole genome shotgun sequence.
ACCESSION   DBNHQR010000244 DBNHQR010000000
VERSION     DBNHQR010000244.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_403506"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1412
                     /gene="phrB"
                     /locus_tag="AC16VR_05665"
     CDS             <1..1412
                     /gene="phrB"
                     /locus_tag="AC16VR_05665"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF007955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HIR6010678.1"
                     /translation="HGRTLMRSIVWFRNDLRVTDNPALVEACARGEVTAVTCLCPAQL
                     RAHDVGPMRIAFLLRSLGVLSHDLASIGVSLKIIERPRFADLPEALVDFARQEGATHL
                     EFNDEYPLDERRRDAEVADACAAAGIVVGRHVADCTLSPGTVLTNSGSPYTVFSPFRR
                     RWLERVDRAQLTPMDAPRRRPGKGAGDPVPVTLDGVGAGLGEDLWPAGEAAAQKALKR
                     FLSGPVLDYGDTRDSPALDATSRLSPYLAIGAISVNQCLHAAVEANGGRLDGRSEGIS
                     KWITELIWREFYRHIVALFPHVSQGKSFRRELDALEWRDAPEDYEAWKAGETGYPLVD
                     AAMRQLNETGWMHNRLRMVTAMFLTKHLLLDWRLGERYFMEKLVDGDFASNNGGWQWS
                     ASTGTDAAPYFRILNPTTQGQRFDADGAFTRRMLPVLADVPDKWLFEPKRFDADLGYP
                     DPIVEHRAARARALALFKN"
     gene            complement(1431..>2090)
                     /locus_tag="AC16VR_05670"
     CDS             complement(1431..>2090)
                     /locus_tag="AC16VR_05670"
                     /inference="COORDINATES: protein motif:HMM:NF052145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA-type ATPase lid domain-containing protein"
                     /protein_id="HIR6010679.1"
                     /translation="ALVQEKLLRVIEYGEFERVGGTKTLHCDVRLVAATNEDLPTRAE
                     EGKFRYDLLDRLAFDVLTLPPLREREEDILLLARHFAITMATELGREYFAGFGSAARD
                     ALMTHPWPGNVRELKNVVERCVYRSHPDEEVEEVVFDPFDSPYRPASQPSPPQGSTPA
                     GNYDLPLDLKQTVRELEIDLINRALENSKHNQRKAADLLKLTYHQLRGYLKKYELLKS
                     D"
BASE COUNT          328 a          664 c          698 g          400 t
ORIGIN      
        1 gccacggccg aactttgatg cgctcgatcg tctggtttcg caatgatctt cgcgtcacgg
       61 acaacccggc gctggtggag gcgtgcgcgc ggggggaggt cacggccgtt acctgtctct
      121 gtcccgccca gctgcgggcg cacgatgtgg gcccgatgcg cattgccttc ctgctgcggt
      181 ccctcggcgt gctctcgcat gacctggcgt ctatcggcgt ttcgctcaaa atcatcgaac
      241 ggccccgctt tgccgacctg ccggaggcgc ttgtcgactt cgcgcggcaa gagggcgcca
      301 cccacctgga gttcaatgac gagtatccgc tggacgagcg cagacgggac gcggaggtcg
      361 ccgacgcgtg cgcggccgcc ggcatcgtcg ttggtcgcca cgtggccgat tgcacgctgt
      421 ctccaggaac ggtgctgaca aacagcggtt ccccctacac ggtgttcagc cccttccggc
      481 gccgctggct ggaacgtgtg gaccgggccc agctgacgcc gatggatgcg ccccgccggc
      541 gcccgggcaa aggcgccggc gatcccgttc cggtaaccct ggacggcgtt ggcgccgggt
      601 tgggtgaaga cctgtggccg gccggggaag cggcggctca gaaggcgctg aagcgctttc
      661 tctccgggcc ggtgctcgac tacggagata cccgtgattc cccggcgctc gatgccacga
      721 gccgcctgtc cccctatctc gccatcgggg cgatatcggt caaccagtgt ctgcacgccg
      781 ctgtcgaagc caacgggggg cgtctcgacg gtaggtccga ggggatatcc aagtggatca
      841 ccgagctcat ctggcgagag ttctatcgcc acatcgtcgc gctgtttccc catgtctccc
      901 aagggaagtc gtttcggcgc gagctcgacg ctctcgaatg gcgggatgcc ccggaggatt
      961 atgaggcctg gaaagccggc gagaccggct atccgctcgt agacgctgcc atgcgccagc
     1021 tcaacgaaac cggctggatg cacaaccggt tgcggatggt gaccgcgatg tttctcacca
     1081 agcacctgct gctggactgg cgactcggtg aacggtactt catggaaaag ctggtcgacg
     1141 gcgacttcgc ctccaacaac ggcgggtggc agtggagtgc gtccacgggc acggatgccg
     1201 ctccgtattt ccggattctc aaccctacga cccagggcca gcggttcgat gctgatggcg
     1261 ccttcacccg gcggatgctg ccggtgctgg ccgacgtgcc ggacaagtgg cttttcgagc
     1321 caaaacgctt tgacgccgac ctcggctacc cggatcccat cgtcgagcat cgcgcggcac
     1381 gcgcgcgggc tctcgcgctt ttcaaaaact gacccgcgcc gcgcgatcag tcagtcggac
     1441 ttgaggagct cgtacttctt gagatagccg cgaagctggt gatacgtgag cttgagaagg
     1501 tcggccgcct tgcgctggtt gtgcttgctg ttctccagcg cgcggttgat caggtcgatc
     1561 tccagctccc gcacggtctg ttttaggtcc agtggcaggt cgtagtttcc tgcgggcgtt
     1621 gagccctgcg gcggggaggg ctgtgacgcg ggccggtagg gtgagtcgaa gggatcgaaa
     1681 acgacctctt cgacctcctc gtcggggtgg ctgcggtata cgcatcgttc cacgacgttc
     1741 ttaagctccc ggacgttgcc gggccatgga tgggtcatca gggcatcccg cgctgcgctg
     1801 ccaaagcccg cgaagtactc gcggcccagc tcggttgcca tcgtgattgc gaagtgcctg
     1861 gcaaggagca ggatgtcttc ctcgcgttcc cgcagggggg gaagcgtgag gacgtcgaac
     1921 gccagccgat cgaggaggtc gtagcggaac ttgccttcct ctgcgcgcgt gggcagatcc
     1981 tcgttggtgg ccgcgaccag ccggacgtcg caatgcagcg tcttggtgcc gcctacccgt
     2041 tcgaactctc cgtactcgat gacgcgcagc agcttttcct gaacgagggc
//
LOCUS       DBNHQR010000245         2738 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_657527, whole genome shotgun sequence.
ACCESSION   DBNHQR010000245 DBNHQR010000000
VERSION     DBNHQR010000245.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_657527"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1641
                     /locus_tag="AC16VR_05675"
     CDS             <1..1641
                     /locus_tag="AC16VR_05675"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA];
                     GO_process: GO:0032787 - monocarboxylic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HIR6010680.1"
                     /translation="AADPPGLARSSPELPVESRAAMTSSMMRLTQGIHRALSLYPRRR
                     AVVSAEGEITWEQFADRVARLAGALRELGVGPGDRVAMLALNSPRYVEYYFAVLWAGG
                     VLAPVNCRWAAPEKAHCLNDSGAKVLLADAAHLPEALELLETCGSVSHLVSCGEVEGS
                     GTAALGYESLVSASLPVEDAERGGADLAALFYTGGTTGRAKGVMLSHDNFIANSMTAL
                     VNLGISEHSVHLHVAPLFHLAGGSRLFTLTVAGGTHAVIPRFDAAEFLGAIERFGVTV
                     TVIVPTMLNTLLQHPNFEAFDLSSLDLLCYGASPMPESLLREAMERLPGARFLQSYGM
                     TELSPVATMLEPQFHTFEGPLAGKIRSAGRAVFNADVAIMSPDDLPLASGEVGEICVR
                     GPMVMQGYWGQPELTAQALRGGWMHTGDAGYLDDDGFLFLVDRVKDMIITGGENVYSA
                     AVENVLYLHPGVHECAVIGVPSDAWGEAVHAIIVPRGGARLDEAAVIEHCRAYLAGYE
                     CPKTVEFVDAELPKSGAGKILKAELRKTYWQGQARNIH"
     gene            1735..>2738
                     /locus_tag="AC16VR_05680"
     CDS             1735..>2738
                     /locus_tag="AC16VR_05680"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6010681.1"
                     /translation="MSNILDRGAKVAPDVEIVTATENGTRRQTYAEIRDRAHQLAHAL
                     RDAGIGVGDRVATFMWNGSRHLEAYHAIGGMGAVLHTLNIRLSAKDLEYIINHAEDRV
                     IIADADVLPLLEPLKGRIPSVERIVVATEDGFEGWSTEVAEAVDYEEFIAGKPTRFDW
                     PRIDENSALGLCYTSGTTGNPKGVEYEHRSQYLHTLTQCMTDSMGLSATDCVLGIVPM
                     FHAMGWGMPWSCLMLGCKQVMPHRFMDTSRLLDLLVSEGVTMSAGVPTIWQGIKALVE
                     ADPERYDLSRLARMACGGSAPPPSLIRWYWDTLGVEMIQGWGMTETSPLATLSRRVMK
                     R"
BASE COUNT          437 a          874 c          927 g          500 t
ORIGIN      
        1 gcggctgatc cgccggggct ggctcgctcg tcgcctgagc tgcctgtaga atcccgcgcc
       61 gccatgactt cctcaatgat gcgcctcacc cagggcattc accgggccct aagcctctac
      121 cctcgccggc gggcggtggt ttcggcggag ggcgagatca cctgggagca gtttgccgat
      181 cgcgtggcgc gcctggcagg cgcgctgaga gagctgggtg tgggtccggg cgatcgagtc
      241 gccatgcttg cgctcaactc gccgcgctat gtggagtact acttcgccgt gctatgggcc
      301 gggggcgttc ttgctccggt caactgccgc tgggcggcgc cagagaaggc gcactgcctc
      361 aacgacagcg gcgcgaaggt tctgcttgcg gacgcggccc acctcccgga agcgctcgag
      421 ctgctcgaaa cctgcgggag cgtctctcat ttggtctcct gcggagaggt ggagggctcg
      481 ggaaccgcgg cgctgggcta cgagtcgctg gtttcggcgt cgctcccggt cgaggacgcc
      541 gagcggggcg gagcggatct ggcagcgctg ttctacacgg gcgggaccac cgggcgtgcc
      601 aaaggcgtga tgctcagcca cgacaacttc atcgccaact cgatgacggc tctggtgaat
      661 ctgggcatct cggagcacag cgtccacctt catgttgcgc cgcttttcca tctggccggc
      721 ggctcccggc tgttcaccct gaccgtggcc ggagggaccc atgcggtgat cccccgcttc
      781 gacgccgccg agttcctggg ggcgatcgag cgcttcgggg tgaccgtcac cgtcatcgtc
      841 ccgaccatgc tcaacacgct gctgcagcat ccgaacttcg aagccttcga tctttcgtcc
      901 ctggatcttc tctgctacgg cgcctcgccc atgcccgaga gccttctgcg tgaagccatg
      961 gagcggctgc cgggagcccg cttcctgcag tcctacggca tgaccgagct gtctcctgtg
     1021 gctacgatgc tcgagccgca gttccatacc ttcgagggtc cgctggcggg gaagatccgt
     1081 tcggccggcc gtgccgtttt caacgcggac gtggccatca tgagccccga cgaccttccg
     1141 cttgcgtcgg gagaggtggg agagatctgt gttcgcggac ccatggtcat gcagggatac
     1201 tggggccagc cggagctcac cgctcaggcg ctgcgcggtg gctggatgca cacgggagac
     1261 gccggttacc tcgacgacga tggttttctg tttctcgtcg atcgggtgaa ggacatgatc
     1321 atcacgggcg gcgagaacgt ctactctgcg gccgtggaga acgtgctgta cctgcacccc
     1381 ggagtgcacg agtgcgcggt gatcggggtc ccgagcgatg cctggggcga ggccgttcac
     1441 gccatcatcg tgcctcgcgg tggcgcccgg ctcgacgagg cggccgtcat cgagcactgc
     1501 cgtgcctacc ttgccggcta cgagtgcccg aagacggtgg agttcgtcga cgccgagctg
     1561 ccgaagagcg gggcgggaaa gatcctgaag gccgagcttc gcaagaccta ctggcagggt
     1621 caggcgcgca acattcactg aggatgatag actcgctgcc ctcacgctga attcaccgga
     1681 acgaaagtct aagggaggtg gcgaaccgtg gcgatgcagc atacgccatt gctgatgtcc
     1741 aatattctgg atcgtggcgc gaaggtggcg cccgacgtgg agatcgtcac cgctaccgaa
     1801 aacggcacgc ggcggcagac ctatgccgag atccgcgatc gtgcccacca gctcgcccac
     1861 gccctgcgtg atgccggcat cggcgtggga gatcgcgtgg cgactttcat gtggaacggg
     1921 tcccgtcatc tggaggcgta tcacgccatc ggcggcatgg gcgcggtgct gcatacgctg
     1981 aacatccgcc tttcggccaa ggacctcgag tacatcatca accacgccga ggatcgcgtg
     2041 atcattgcgg atgcggacgt gttgccgctg ctggagccgc tcaagggacg gattcccagc
     2101 gtggagcgga tcgtggtcgc caccgaggat ggtttcgaag gctggagcac cgaggtggcc
     2161 gaggccgtcg actacgagga attcattgcc ggcaagccga cccgtttcga ctggccccgg
     2221 atcgacgaga actccgccct cggcctgtgc tacaccagcg gcaccacggg caacccgaag
     2281 ggcgtcgagt acgagcaccg ttctcagtat ctgcacacgc tcactcagtg catgaccgac
     2341 tccatgggcc tctccgccac ggattgcgtg ctcggcatcg tgcccatgtt tcacgccatg
     2401 ggctggggca tgccctggtc gtgcctcatg ctgggctgca agcaggtcat gccgcaccgg
     2461 ttcatggata cgtcgaggct gctggatctg ctggtgtccg agggcgtgac catgtcagcc
     2521 ggcgtgccga ccatctggca gggcatcaaa gcgctggtcg aggccgaccc ggagcgctac
     2581 gacctttccc gcctggctcg catggcctgc ggcggctccg cgccgccgcc gtcgctgatc
     2641 cgctggtact gggacaccct cggggtagag atgattcagg gatggggcat gaccgagacg
     2701 tcgccgctgg ccacgctgtc gcggcgggtg atgaagcg
//
LOCUS       DBNHQR010000246         1642 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_657550, whole genome shotgun sequence.
ACCESSION   DBNHQR010000246 DBNHQR010000000
VERSION     DBNHQR010000246.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_657550"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /gene="rodA"
                     /locus_tag="AC16VR_05685"
     CDS             <1..1022
                     /gene="rodA"
                     /locus_tag="AC16VR_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HIR6010682.1"
                     /translation="IVGYGLVVLYSALNGNEELFSNQAVRIGLALSVMLVAAQLEPRF
                     YLRWSPVVYGVGLVLLVLVLLIGVKVKGSQRWLDLPGLPRFQPSELMKIAVPMMVAWY
                     FHERRLPPSFRDVCVAIVIIGLPAGCIILQPDLGTGILVACAGFAVVVLAGVYWRWIA
                     FAAFGIAALAPGLWFLLQDYQRQRILTLFDPESDPLGAGWNIIQSTTAIGSGGVWGKG
                     LMQGTQSRLDFLPESQTDFIIAVIGEELGLVGIASLLVLYLVVVARGLYIATRAVNTY
                     QRLLAGALTLTFFVYVFVNIAMVSGLLPVVGVPLPLVSYGGTSALTLMARFGIIMAIR
                     TERAW"
     gene            1079..>1642
                     /locus_tag="AC16VR_05690"
     CDS             1079..>1642
                     /locus_tag="AC16VR_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019824216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HIR6010683.1"
                     /translation="MRYAFLWATTLLLLFGSAHADFAGRAEVREYIAEMVREHGFGEE
                     ELADVFADADRKESILEAIARPAERVLKWHEYRRIFLGEKRISQGIEFWREHAETLAR
                     AEAAYGVPPEYVVAIIGVETRYGRNRGGYRVVDALTTLAFDYPPRSAFFRDELTHFLL
                     LAREEGMDPRDPKGSYAGAMGYGQFIPS"
BASE COUNT          241 a          506 c          534 g          361 t
ORIGIN      
        1 ccatcgtggg ctacggactg gtggtcctct acagcgccct gaacggcaac gaggagctgt
       61 tttcgaatca ggctgtgcgc atcggcctgg cgctctcggt gatgctggtg gcagcgcagc
      121 tggagccgcg cttctatctg cgctggtcgc ccgtcgtcta cggcgtgggc ctggtgcttc
      181 tcgtgctcgt cctcctaatc ggtgtgaagg tcaaaggctc ccagcggtgg ctcgacctgc
      241 ctggactgcc cagattccag ccttcggagc tcatgaagat cgccgtgccc atgatggtgg
      301 cctggtactt ccatgagcga cgcctgccgc cgagcttccg cgacgtctgt gtcgccatcg
      361 tcatcatcgg cctgccggcc ggttgcatca tcctgcagcc ggatctggga acgggcatcc
      421 tcgtggcctg cgcgggcttt gccgtggtgg tgctggccgg cgtctactgg cgatggatag
      481 ccttcgccgc gttcggcatc gccgcccttg ccccgggcct ttggttcctg cttcaggact
      541 atcagcgaca gcggatcctc accctgtttg atcccgaaag cgatcccctg ggcgccggct
      601 ggaacatcat tcagtcgacg acggccatcg ggtctggcgg cgtgtggggc aaggggctga
      661 tgcaaggcac gcagagccgc ctggatttcc tgcccgagag ccagacggat ttcatcatcg
      721 ccgtcatcgg cgaggagctc gggctggttg gcattgcctc tctgctcgtt ctttatctcg
      781 tcgtggtcgc ccgggggctg tacatcgcga cgcgcgcggt gaatacgtat cagcgcctgc
      841 tggcaggcgc gctgacgctg acgttcttcg tttacgtgtt cgtcaacatc gctatggtga
      901 gcgggctgct gccggtcgtc ggcgtgccgc tgccgctggt cagctacgga ggcacgtctg
      961 cgctcacgct catggcgaga tttggcatca tcatggcaat ccgcaccgag agggcatggt
     1021 agtgcgcctg gtttcgggtc ccttctttca cattggacac gttcggatag gtagattcat
     1081 gcgatacgct tttctctggg caaccaccct ccttctcctg ttcggctccg cgcacgcgga
     1141 ctttgccggt cgggctgagg tacgcgagta cattgccgag atggtccggg agcacggttt
     1201 cggggaggag gaacttgccg acgtctttgc cgatgccgat cgcaaagaaa gcatcctcga
     1261 ggccattgcc cgtcccgccg agcgggtgct gaagtggcac gagtatcggc ggatattcct
     1321 cggagagaag cgcatctctc agggtatcga gttctggcgc gagcacgccg aaaccctggc
     1381 acgagcagaa gcggcctatg gcgtgccgcc cgagtacgtc gtggcgatca tcggggtgga
     1441 gactcgctac gggcgcaatc ggggtggata ccgggtcgtg gacgctctga cgacgctcgc
     1501 ctttgactac ccgccgcgct ccgctttctt tcgtgacgag ctcacccatt ttctgctgct
     1561 cgcccgggag gagggcatgg atccgcggga tccgaaaggc tcctatgccg gcgccatggg
     1621 atacggtcag ttcattcctt cc
//
LOCUS       DBNHQR010000247         1770 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1670649, whole genome shotgun sequence.
ACCESSION   DBNHQR010000247 DBNHQR010000000
VERSION     DBNHQR010000247.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1670649"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="AC16VR_05695"
     CDS             complement(<1..967)
                     /locus_tag="AC16VR_05695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR6010684.1"
                     /translation="MKIIYVVNEGRFFLSHRLALAVEAKRHEVEVVVVCGANTGEQGL
                     ADAGLRYVTVPMSRSGFNPFKELRTAAALLRIYRAEKPDLVHHVTIKPVLYGTSMARW
                     SGVPAVVNAVPGMGFVFTRRGLLARIRRSVVQLLYRLCMFHPNMRVIFQNNEDMQAFT
                     QRGIVDRQHVALIRGSGVDLDVFRCAPEPPGPVTFVLVGRMLRHKGVYEYVEAARRVR
                     QRRPDWRFQLVGDVDPGNPASLRPDELIAWQQAGEIEWLGQRRDVPEVLAAAHVVCLP
                     SYREGLPKTLLEAAACGRAMIASDIAGCREVVRPNVTGLLVPPRVS"
     gene            complement(1267..1548)
                     /locus_tag="AC16VR_05700"
     CDS             complement(1267..1548)
                     /locus_tag="AC16VR_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HIR6010685.1"
                     /translation="MTMTIKRAMGAALLLAGVLAGPVLAAPESVNINSANAETLAEVL
                     NGVGLTKAQAIVEYREQHGAFRDAYELANIKGIGERTVEKNEARIRLQD"
     gene            complement(1674..>1770)
                     /locus_tag="AC16VR_05705"
     CDS             complement(1674..>1770)
                     /locus_tag="AC16VR_05705"
                     /inference="COORDINATES: protein motif:HMM:NF037181.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010686.1"
                     /translation="CGFSGHTLIWQCPSCKTWGSIKPIVRYDGDV"
BASE COUNT          328 a          608 c          551 g          283 t
ORIGIN      
        1 cgctgacccg gggcgggacc agcagtcccg tcacgttcgg gcggaccacc tcccggcatc
       61 cggcgatgtc cgaggcgatc atggcccgcc cgcaggcggc cgcttcgaga agcgtcttgg
      121 gcagcccctc ccgataggac ggcaggcaga ccacgtgagc cgcagccagg acctcgggaa
      181 catcgcgccg ttgtccgagc cactcaatct cgccggcctg ctgccaggcg atcagctcat
      241 cgggccgcag cgatgcagga tttccaggat ccacgtcgcc gacgagctgg aatcgccagt
      301 cggggcgccg ttgcctgacc cggcgggcgg cctcgacgta ctcgtaaacc cctttgtgcc
      361 ggagcatccg tccaacgagc acgaacgtca cggggccggg cggctcggga gcgcaccgga
      421 acacgtccag atcgacgccc gatccccgaa tcagcgcaac gtgctgccgg tctacgatgc
      481 cccgctgggt gaacgcctgc atgtcctcgt tgttctggaa tatcacgcgc atgttgggat
      541 ggaacatgca caaacggtaa agcagctgca cgacgcttcg tcggatgcgg gcaagcagcc
      601 cgcgccgggt aaacacgaac cccatccccg gcacggcgtt caccacggcg ggcacgccgc
      661 tccagcgcgc catggaagtg ccgtaaagaa ccggcttgat ggtcacgtgg tgaaccaggt
      721 cgggcttctc ggcacggtag attcgcagca gcgcggcggc ggtgcgcagt tccttgaacg
      781 gattgaaacc gcttcgcgac atgggcaccg taacgtagcg cagccccgcg tccgccagtc
      841 cctgctcccc ggtattggcg ccgcaaacca ccaccacctc cacctcgtga cgttttgctt
      901 ccacggccaa agccaggcgg tgagagagaa agaaccgtcc ttcgttgacc acgtagatga
      961 tcttcatcac ccgatcatcc atatgagcag gtcggcgccg gtacccgcgc cggcagaggt
     1021 gggcattagg ggcaaacgct gcaaagaatc catacttcgt cgcgacgcca tcggcggctt
     1081 tcgtgctcca tcttaccgcc cgcgccccgg caatgagagg tgctccggac aatgcgcttg
     1141 accggcgtca caaaaacgcc gacgagcggc cagactccgg ggcaagcctg ccacccggag
     1201 cctggcctgg cgaactcagg acgacgcccg gcgaacgccg catcgaaggt gcctcaagcc
     1261 gcagggtcaa tcctgcaggc ggatccgcgc ctcgttcttc tcgacggtcc gttcgccgat
     1321 gcccttgatg ttcgcaagct catatgcgtc gcggaacgcg ccgtgctgct cacggtactc
     1381 gacgatggcc tgggccttgg tgagcccaac gccgttcagc acctcggcaa gcgtctccgc
     1441 attcgcgctg ttgatgttca cactctccgg ggccgcgagc accggcccgg cgagcacacc
     1501 ggccagcagc agcgcagcgc ccatcgcacg tttgatcgtc atcgtcatgg gtatctccgt
     1561 tatcagtggt gaggaggacg acaatgctac gaggcgccca ggcgcccgcg taagccgcaa
     1621 agagccgccg tttccgtaag ttcggacccg gcgaggacgc gggccaatgg agttcagacg
     1681 tcgccgtcgt aacgcacgat gggcttgatg ctgccccagg tcttgcagct cggacactgc
     1741 cagatgagcg tgtgcccgct gaagccacag
//
LOCUS       DBNHQR010000248         4558 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_403622, whole genome shotgun sequence.
ACCESSION   DBNHQR010000248 DBNHQR010000000
VERSION     DBNHQR010000248.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4558)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_403622"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1257
                     /locus_tag="AC16VR_05710"
     CDS             <1..1257
                     /locus_tag="AC16VR_05710"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6010687.1"
                     /translation="RDYMQQEWDNLWTKIWHIAGREKQLAEPGDFIVHDFMRESVIIV
                     RQRDGSLRGFYNACGHRAQRLVWNHGHQESFLCPYHGWQWGLDGSLEDLPDRDDYPQG
                     DPVGKLGLIELRVDTWAGLVWYTMDDEAPDLMKYLEPLPEIYRSFQFEKTVRSRWLRV
                     ELECNWKFWSDNFNESYHTRTAHPQVAPIIDQDHFTSRYEMFPMGHARIIQMGRPSLR
                     DRVPEGQPHPWDKELRFWGIDPDGYPDYETKVMQGWLDLKAAKRTRWKEKGFHHYEHL
                     TDEELTESPFQTVFPNIAFGASADSFGVFRWEPHPTDPEKCYFDIWSMTYPVEGIEET
                     VHRTARNPLKFEEAEYEFRVYDNGQGVLDLSDQVVFQDWMLNAGQTTGWHSRGYQEPY
                     LAAQETRVRRFHEVLHDYLAGNPPGR"
     gene            1332..1709
                     /locus_tag="AC16VR_05715"
     CDS             1332..1709
                     /locus_tag="AC16VR_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010688.1"
                     /translation="MPRFVLIGLCEPPNPESQQQFDEWFVDQHIEDTAKCPNFIRGSV
                     YKLSGPHLDGETVSGYLSLYEVEAPSYPEAERVLNEWQANPEAWEGRKHHLATAQKLG
                     GMPLAIKGSGWYELLKSFDGPAA"
     gene            complement(1729..2520)
                     /locus_tag="AC16VR_05720"
     CDS             complement(1729..2520)
                     /locus_tag="AC16VR_05720"
                     /inference="COORDINATES: protein motif:HMM:NF021465.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HIR6010689.1"
                     /translation="MSRDPASEGRVSIRTLDQLALWSSLSIVVALSWWYLFLLAQDMA
                     DMAGMPMGDMAGMHPWTALDFLLMFLMWAVMMVGMMVPTAVRAIYIYARVASQAQARG
                     SPVAATQIFVAGYVLVWAAFSLGATALQAALDALGLLSPAMRSSSPLLGAGLLVAAGV
                     YQLTPWKDTCLRHCQSPAAYLAGRFGPRALDALSLGIRHGLYCLGCCWVLMLLLFLGG
                     VMNLLWIAAIAAFVLLEKVLPPALRVSRLAAVMMIASGAAYLMIA"
     gene            complement(2517..3158)
                     /locus_tag="AC16VR_05725"
     CDS             complement(2517..3158)
                     /locus_tag="AC16VR_05725"
                     /inference="COORDINATES: protein motif:HMM:NF018712.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HIR6010690.1"
                     /translation="MTDHWHFHSDTFGNCNCAQNCGCQFNLPSTHGFCQFVEGGHIRD
                     GSFNGMNISGFNWAFMMIWPGEIAEGNGRRQIVIDERANDQQRAAIEKIVSGEVGIPG
                     GNHFSVFGSTCSESLETLYLPIEFEMDIAARRARLAIPGLIEARGEPMMDGFSGSEFH
                     IALARPKGSFEFTYAEIGQGFAEVHGELPMSLQGSYGQFCEHNYDHDGLVIPA"
     gene            3329..3673
                     /locus_tag="AC16VR_05730"
     CDS             3329..3673
                     /locus_tag="AC16VR_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014422345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HIR6010691.1"
                     /translation="MRRAIGFLILTTVTTVPAWAGDKLEAAVGGGAGGAIGAVVGDEL
                     GDRKGAIIGSAVGAAIGTAIATDGNDGGPDHHHVEAHTAPASTVHVEVGSAGGHPAGR
                     HCPPGQAKKGRC"
     gene            3828..>4558
                     /locus_tag="AC16VR_05735"
     CDS             3828..>4558
                     /locus_tag="AC16VR_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010692.1"
                     /translation="MLEAVNVSAEVVAAILGISITVVAIIVQLAATRYNHHITEMFVR
                     EPINVVVQAFFVLTTVLCIWVAALGEAELNATWNTVTLVTVTVALAALLPYFGYVFAF
                     ISPVGIIRRIRRKAEHAVAAGHRAETLRTIDQLQDVARSAIDHGDRAIAMAAVEALSD
                     LFRHYRSVRNRLPEGWSRVDGIVREDPDFVALEPSALDDLDTSGLWVEVKIMHQYVSI
                     IRLSVMKMHDVAGRVGIALRQTAIE"
BASE COUNT          858 a         1525 c         1443 g          732 t
ORIGIN      
        1 cgcgattaca tgcagcagga atgggacaac ctgtggacga aaatctggca catcgccgga
       61 cgcgagaagc agctggcgga gcccggggac ttcatcgttc acgacttcat gcgcgagtcg
      121 gtgatcatcg ttcgccagcg ggacggctcc ctgcgcgggt tctacaacgc ctgcggacac
      181 cgcgcccagc ggctcgtgtg gaaccatggc caccaggaga gtttcttgtg cccctatcac
      241 ggctggcagt gggggcttga cgggtctctg gaggacctgc ccgaccggga cgactatccc
      301 cagggagacc ccgtgggcaa actgggcctg atcgagctgc gcgtggatac ctgggccggg
      361 ctcgtctggt acaccatgga cgacgaagcg ccggacctga tgaagtatct cgagcccctt
      421 ccggagatct accgcagctt ccagttcgag aagacggtac gctcccgctg gctgcgggta
      481 gagctcgagt gcaactggaa attctggtcg gacaacttca acgagtccta ccacacccgc
      541 accgctcatc cccaggtagc acccatcatc gaccaggatc acttcacctc gcgctacgag
      601 atgtttccca tggggcacgc gcgcatcatc cagatgggcc gcccgtcgct tcgcgaccgg
      661 gttcccgagg gccagccgca cccgtgggac aaggagctgc gcttctgggg catcgacccc
      721 gacggctatc ccgactacga gaccaaggtc atgcagggat ggctcgatct caaagccgcc
      781 aagcgcacgc gctggaagga gaaaggcttt caccactacg agcacctgac cgacgaggaa
      841 ctgacggaga gccccttcca gacggtcttt cccaacatcg ccttcggcgc ctcggccgat
      901 tcgttcggcg tcttccgctg ggagccgcac ccgaccgacc cggagaagtg ctacttcgac
      961 atctggagca tgacctaccc ggtggagggc atcgaggaga ccgtgcaccg cacggcgcgc
     1021 aacccgctca agttcgagga ggccgagtac gaattccgcg tctacgacaa cggccagggc
     1081 gttctcgacc tctccgacca ggtggtcttc caggactgga tgctcaacgc cggccagacc
     1141 acgggctggc attccagggg ctatcaggaa ccgtacctgg cggcccagga aaccagggtc
     1201 cggcggttcc acgaggtgct gcacgactac ctggcgggga acccgccggg gcgctgagcc
     1261 gaggggtcac ggcgctctcc acacgagggc gctccgtccc ccttacgtat ttgacaagtc
     1321 aggacacaca aatgccgaga ttcgttctca tcgggctctg cgagccgccc aaccccgaat
     1381 cccaacagca gttcgacgag tggttcgtcg accagcacat cgaggatacc gccaagtgcc
     1441 ccaacttcat ccggggcagc gtctacaagc tgagcgggcc tcacctggac ggcgagaccg
     1501 tttccggata cctctcgctc tacgaggtgg aggcccccag ctacccggaa gccgagcggg
     1561 tgctcaacga gtggcaggca aatccggagg cctgggaggg acgcaagcac cacctggcca
     1621 ccgcccagaa gctcgggggc atgccgctgg ccatcaaagg ctcgggctgg tacgagctgc
     1681 tgaagtcgtt cgacgggccc gccgcctgaa cgttagcgac tcgagcggct aggcgatcat
     1741 caggtaggcg gcgccgcttg cgatcatcat cacggcagca agccgggata cacgcagcgc
     1801 gggaggcagg actttctcca gcagcacgaa cgccgcgatg gccgcgatcc acagcaggtt
     1861 catcacgccg ccgagaaaga gcagcagcat cagcacccag cagcagccaa ggcagtaaag
     1921 gccgtgccgg atacccaggc tcagagcgtc cagcgctcgc ggcccgaagc gtccggccag
     1981 ataagcggcc ggggactggc agtggcgcag gcaggtgtcc ttccacgggg tgagctggta
     2041 cacgcccgcg gcaacgagca acccggcgcc cagcagcggg ctggacgagc gcatggccgg
     2101 tgacagcagg cccaatgcgt ccagcgcggc ctgcaacgcc gtcgcgccga ggctgaaagc
     2161 ggcccagacc agcacgtagc cggccacgaa gatctgggtg gcggccaccg gcgagccccg
     2221 ggcttgggcc tggctcgcaa cccgcgcgta gatgtagatg gcgcgcactg cagtgggcac
     2281 catcatgccc accatcatca cggcccacat caagaacatg agcaggaagt ccagcgcggt
     2341 ccaaggatgc atgcccgcca tgtcgcccat gggcattcca gccatgtcgg ccatgtcttg
     2401 ggccagcagg aacaggtacc accaggacag cgctaccacg atgctcagcg acgaccagag
     2461 ggcaagctga tcgagggtcc ggatcgagac gcgcccttcc gacgccggat ctcggctcac
     2521 gcgggaatga cgagcccgtc gtgatcgtag ttgtgctcac agaactggcc gtagctgccc
     2581 tgcagcgaca tgggcagctc gccatgcacc tccgcgaatc cctggccgat ctccgcatag
     2641 gtgaactcga agctgccctt gggcctcgcc agcgcgatgt gaaactcgct gccgctgaac
     2701 ccgtccatca tgggttcgcc tctggcctcg atgaggccgg ggatcgccag ccgcgcgcgg
     2761 cgagcggcaa tgtccatctc gaactcgatg ggcaggtaga gggtttccag cgattcggag
     2821 caggtgctgc cgaacactga gaaatggttg ccgccgggga tgcccacctc gccggagacg
     2881 atcttctcga tggccgctcg ctgctggtcg tttgcgcgct cgtcaatgac gatctggcgg
     2941 cggccgttgc cctcggcgat ctcacctggc cagatcatca tgaacgccca gttgaaccct
     3001 gagatgttca tcccgttgaa ggatccgtcg cggatgtgac caccctcgac gaactgacag
     3061 aatccatggg tgctggggag gttgaactgg catccgcagt tctgggcgca gttgcagttg
     3121 ccgaaggtgt cgctgtggaa gtgccagtga tcggtcatga cgatgtcccc ttgccaactt
     3181 ctcgaccata catcagacac cctgggatca ctagcacgcc gcgaggctca cggcgcgcgc
     3241 gcaagggaga atccatgacc caggtcacga ttctgctgtc gacgcccccc tacactcccg
     3301 gggttgtttc aaaaagcagg ggcctgtcat gcgccgagcc attggatttc tgattctcac
     3361 caccgttacg accgtgccgg catgggccgg cgacaagctc gaggccgccg taggcggcgg
     3421 tgccggaggc gccatcggcg ccgtggtcgg cgacgagctg ggcgatcgta agggagcgat
     3481 catcggcagc gcggtgggcg cagccatcgg cacggccatc gccaccgatg gcaacgatgg
     3541 cgggcccgac catcaccacg tggaggcgca cacggcacct gccagcaccg tgcatgtgga
     3601 agtgggctct gccgggggac atcccgcagg acgccactgc ccgcccggcc aggccaagaa
     3661 gggtcgctgc tgatatcgag atagggatcg gggacggcat tttccagccg tcccctgcct
     3721 ccggcaggat ccggttccgg agaaccgaca tcaggcgtaa ccgcacgcat cgcgccttct
     3781 gacgacttgc ggtaggcttg cgtcatcgca agcaccggac ccgatccgtg ctggaagccg
     3841 taaacgtttc tgccgaggtc gtcgccgcga ttctcggcat ctccatcacg gtggtcgcca
     3901 tcatcgtgca gctggccgcg acccgctaca accaccacat caccgagatg ttcgtccgcg
     3961 agcccatcaa cgtggtggtt caggccttct tcgtgctcac caccgtcctg tgcatctggg
     4021 tcgccgccct gggcgaggcg gagctgaacg ccacctggaa caccgtcacc ctggttaccg
     4081 ttaccgttgc cctggccgcg ctgctgccct acttcggcta cgtcttcgcc ttcatctcgc
     4141 cggtcggcat catccgacgc atccgccgca aggcggagca tgccgtcgcc gccggccacc
     4201 gcgcggagac ccttcgaacc atagaccagc ttcaggacgt cgcccgcagc gccatcgatc
     4261 acggcgaccg tgccatcgcc atggccgcgg tggaggcgct gtccgacctg ttccggcatt
     4321 accgctccgt gcgcaaccgg ctgcccgagg ggtggtcccg ggtcgacggg atcgtgcggg
     4381 aggaccctga cttcgtcgcc ctggagccca gcgccctgga cgacctggac acctcggggc
     4441 tttgggtcga ggtcaagatc atgcatcagt acgtcagcat catccggctg tccgtgatga
     4501 agatgcatga cgtggccggt cgggtcggca tcgccctgcg gcagacggcc attgagca
//
LOCUS       DBNHQR010000249         3934 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1100963, whole genome shotgun sequence.
ACCESSION   DBNHQR010000249 DBNHQR010000000
VERSION     DBNHQR010000249.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1100963"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="AC16VR_05740"
     CDS             complement(<1..255)
                     /locus_tag="AC16VR_05740"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR6010693.1"
                     /translation="MKLLVTGLSGLIGGAAHPVLAADHDVAALNRREVNGTFTWQADI
                     ADYEAIRPAFDGVDAVIHLAAKPGEHFGWDALRVTNVEGTR"
     gene            complement(252..1211)
                     /locus_tag="AC16VR_05745"
     CDS             complement(252..1211)
                     /locus_tag="AC16VR_05745"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6010694.1"
                     /translation="MDEIVPRHIRDPRIETTFVDANGLRFEVDACGSGRRLALCLHGF
                     PEHSFSWRHQLPMLADLGFRAWAPNLRGYGLTSRPFGVDAYAIENLMDDVAGLIDASG
                     CRETVLIAHDWGALVAWYFAMRKVRPLEKLIICNVPHPGAGRASGRSFAQLRKSWYVF
                     FFQLPRLPENLLGRRGARAVADAFRGVAVDPSRFPDEVVDVYRRNAAQPGALEAMINY
                     YRAALRGGGFRRQSALGFPVIETPTLMVWGEEDAALGIETTRGTDDYVANLTLRYLPR
                     VSHWVQQEQPEVVNAMMRAFLENEPVPYLTWEPKLVQAVKEEA"
     gene            complement(1204..1875)
                     /locus_tag="AC16VR_05750"
     CDS             complement(1204..1875)
                     /locus_tag="AC16VR_05750"
                     /inference="COORDINATES: protein motif:HMM:NF044444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6010695.1"
                     /translation="MNVKSTRVRRNPEAARAEILAAAMAALQDVRFSELTVDSLMKRT
                     GMTRSSFYHYFSGLDEIALGMLEEFAADIRRTVDPWLTGELDESDHRAATLTHLTSMF
                     EVFERHAPSVAAVAQAAGGSQEVYQEWQRGIVDYFIDLTAEFIRRQRALGRSGAADPD
                     RLARALILMNNAAANDNMTRHNPDGPAVIGAVLGDIWNSAIYGPGGDGTSGCPVELKG
                     GSIRG"
     gene            2000..3526
                     /locus_tag="AC16VR_05755"
     CDS             2000..3526
                     /locus_tag="AC16VR_05755"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6010696.1"
                     /translation="MNVLASQLEPWAVADRQPRIAIIGAGMSGIAAVVKLRKAGYTHL
                     TVYEKADRVGGTWRENTYPGLSCDVPSRWYSFSFALSSEWTHRFSYGPEIQAYMEKVA
                     EGFGVTDVVRFDTPVTELTYEAPFWRLTTAAGEQQIYDVVISATGILHHPAMPDIEGL
                     EDFAGASFHSARWDHSVDLTGKRVGVIGTGSTAAQIVGDIAGSVGELHVFQRTPQWMV
                     PMPQKTYSARWKRVLKLFPFLKRLAYQYYFRLMVRTFAMATVGDEKMQARISKITRQN
                     LERNVKDPELRRRLTPDYQAACKRLILCSDFYPAITRDNAHLVTDAIERVTPAGIRTA
                     DGRVHELDVLVLATGFDPSAFILPTKVTGEDGVDLESFWQGAPRALRAVAIPRFPNFW
                     MLEGPTGPVGNMSLITITEHQVGYVIAMLERMKKRRLVAVSAREDAYRNYNQAMQEAV
                     KNTVWMTGGCTSWYIDKTGIPNLYPWEPMRYLKSMRNPDFSEYREETGQGKEAALAAA
                     "
     gene            complement(3531..>3934)
                     /locus_tag="AC16VR_05760"
     CDS             complement(3531..>3934)
                     /locus_tag="AC16VR_05760"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024591.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR6010697.1"
                     /translation="TTAAAFSDGWFHTGDIGHMDAEGFFYVDDRKKDVVISGGENVYP
                     AELENVLAEMPELAEYAVVGRPDERWGEVPVACVVLRDGADLGEDELIARFAGRLARY
                     KHPREVRFFQSLPRNVMGKVLKFRLREEVAP"
BASE COUNT          727 a         1347 c         1218 g          642 t
ORIGIN      
        1 gcgcgttccc tccacgttgg tgacgcgcaa agcgtcccag ccgaagtgct caccgggctt
       61 cgccgccagg tgaatgacgg cgtcgacccc gtcgaacgct ggccgaatgg cttcgtagtc
      121 ggcaatgtcg gcctgccagg tgaacgtgcc gttcacctcc cggcggttca gcgccgcgac
      181 gtcgtggtcg gctgcaagaa ccgggtgggc agcgccaccg atgagcccgc tcaagcccgt
      241 gacgagaagc ttcacgcttc ctcctttacg gcctgcacga gcttcggctc ccaggtgagg
      301 tagggaaccg gctcgttctc gagaaaagct cgcatcatgg cattgaccac ctcgggctgc
      361 tcctgctgaa cccagtgcga gacccgcggc aggtagcgca gggtgaggtt cgcgacgtag
      421 tcgtcggtgc cacgcgtggt ctcgatgccg agcgcagcgt cctcctcgcc ccagaccatg
      481 agggtggggg tctcgatgac gggaaagccg agcgcgctct ggcggcgaaa accgccgccg
      541 cgcaaggccg cccggtaata gttgatcatg gcttccagcg cgccgggctg cgccgcgttg
      601 cgccggtaaa cgtccaccac ctcgtccggg aagcggctcg gatccacggc gaccccccga
      661 aacgcatccg ccacggcccg tgcgcctcgg cgacccagaa gattctcggg aaggcgggga
      721 agctgaaaga agaacacgta ccaggacttc cggagctgag cgaacgatcg ccccgatgct
      781 ctgcctgccc cgggatgcgg aacgttacag atgatcagct tttccagggg acggaccttg
      841 cgcatggcga agtaccaggc caccagggct ccccagtcgt gggcgatgag caccgtctcc
      901 cggcaaccgg acgcatcgat cagccctgcc acgtcgtcca tgaggttctc gatggcgtaa
      961 gcgtccaccc cgaagggccg cgacgtcagg ccgtaacccc tcaagttcgg cgcccaggcc
     1021 cgaaagccga gatctgccag catgggcagc tgatgacgcc aggagaagct gtgctccgga
     1081 aagccgtgca gacacagcgc gaggcgccgg ccggagccac aggcgtccac ctcgaacctg
     1141 aggccgttgg catcgacgaa cgtcgtttcg atgcggggat cccgtatgtg ccgaggaacg
     1201 atctcatcca cgaatgctgc ctcccttcaa ctcaaccggg cacccgctgg tgccatcgcc
     1261 tcccgggccg tagatggccg agttccagat atcgccgaga acggcgccga tcaccgccgg
     1321 cccatcggga ttgtggcggg tcatgttgtc gtttgccgcg gcgttgttca tcagaatcag
     1381 cgcccgggcc agtcggtcag gatcggcggc cccgcttcga cccagcgccc gctgccggcg
     1441 aatgaactcc gccgtgagat cgatgaagta atcgacgatc ccccgctgcc actcctgata
     1501 gacctcctgg ctcccgccgg ccgcctgggc aacggcggca acgctgggcg cgtgccgttc
     1561 gaacacctcg aacatcgagg tgagatgggt aagcgtcgcg gcgcggtgat cggactcatc
     1621 cagttccccc gtgagccagg gatctaccgt gcggcgaatg tccgccgcga actcctcgag
     1681 catacccagc gcaatctcgt ccaggccgga aaagtagtgg tagaaagagg aacgcgtcat
     1741 gcccgtccgc ttcatcaagg aatccacggt cagctcggag aagcggacgt cctgcagggc
     1801 ggccatggcc gccgcgagga tctccgcccg cgccgcctcc ggattgcgcc tgacccgcgt
     1861 gctcttgacg ttcatgacct ccattcccgg aatgcttcag tcttccaccc ggacattcta
     1921 ccccgggcaa tccactattg acacgatgtc aatatataac ctattttatt gacgatgtgt
     1981 caataaaggc gggggcggca tgaacgtact ggcatctcag ctggaaccct gggcggtggc
     2041 cgatcgccag ccccgcatcg ccatcatcgg ggccggcatg tccggcatcg ccgccgtcgt
     2101 caagctccgc aaagccggtt acacccacct caccgtttac gagaaggccg accgggtcgg
     2161 cgggacctgg cgcgagaaca cctacccggg gctgtcctgc gacgtgccct cacgctggta
     2221 ctcgttcagc tttgccctga gctccgagtg gactcaccga ttctcctacg gcccggagat
     2281 ccaggcctac atggagaagg tcgccgaggg cttcggcgtg acggacgtgg tccgtttcga
     2341 caccccggtg acggagctca cctacgaagc accgttctgg cgcctcacta ccgccgcggg
     2401 cgagcagcag atctacgacg tcgtgatctc ggccacgggc atcctccacc atcccgccat
     2461 gccggacatc gaaggactgg aggatttcgc aggagccagc tttcactcgg cccgctggga
     2521 tcactcggtg gatctcacgg gcaagcgtgt gggcgtgatc ggcaccgggt ccacggcagc
     2581 ccagatcgtc ggcgacatcg ccggctccgt tggcgagctc cacgtattcc agagaacgcc
     2641 ccagtggatg gtgcccatgc cgcagaagac ctacagcgcc cgctggaagc gggtgctcaa
     2701 gctcttcccg ttcttgaagc gcctggccta tcagtactac ttccgcctca tggttcgcac
     2761 cttcgccatg gccaccgtgg gcgatgagaa gatgcaggcc cgcatcagca aaatcacccg
     2821 gcagaacctc gagcgcaacg taaaggaccc ggagctgcgc cgcaggctga cgcccgacta
     2881 ccaggcggcc tgcaagcgcc tcatcctctg ctcggacttc tacccagcca tcacccgcga
     2941 caacgcccac ctggtcacgg acgccatcga gcgcgtcacc cccgccggca tccgaaccgc
     3001 cgacggtcgc gtccacgagc tcgacgttct cgtcctggcc accgggttcg atccgagcgc
     3061 gttcattctg ccgaccaagg tgacgggtga agacggggtg gacctggagt ccttctggca
     3121 gggcgcccca cgagcactga gagccgtggc gattccccgc tttcccaact tctggatgct
     3181 ggagggccct acgggccccg tgggcaacat gtccttgatc accatcaccg agcatcaggt
     3241 gggctacgtc atcgcgatgc tcgagcgcat gaagaagcgc cggctcgtgg ccgtctccgc
     3301 gcgcgaggac gcctaccgga actacaacca ggccatgcag gaggcggtga agaacacggt
     3361 ctggatgacg ggcggctgca cgagctggta catcgacaag accgggatcc cgaacctgta
     3421 cccctgggag ccgatgcgct atctgaagtc catgcgcaat cccgacttct cggaataccg
     3481 ggaggaaacc gggcagggga aggaagcggc gctggcagcg gcctagaggg tcagggggcg
     3541 acctcctcgc gcagcctgaa cttgagcacc ttacccatga cgttgcgcgg cagcgactgg
     3601 aagaagcgca cttcccgggg atgcttgtaa cgggcaagac gtccggcgaa gcgcgcgatc
     3661 agctcatctt cacccaggtc ggccccgtcc cgcagcacca cgcaggcaac cgggacctcg
     3721 ccccagcgct cgtccggccg gcccaccacc gcgtactctg cgagctcggg catctccgcc
     3781 agcacgttct cgagctcggc agggtagacg ttctcaccgc cggagatgac cacgtccttc
     3841 ttccgatcgt ccacgtagaa gaacccttcg gcgtccatgt ggccgatgtc cccggtatga
     3901 aaccagccgt cggagaacgc ggcggccgtg gtct
//
LOCUS       DBNHQR010000250         4087 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_150554, whole genome shotgun sequence.
ACCESSION   DBNHQR010000250 DBNHQR010000000
VERSION     DBNHQR010000250.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_150554"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..165
                     /locus_tag="AC16VR_05765"
     CDS             19..165
                     /locus_tag="AC16VR_05765"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6010698.1"
                     /translation="MKARTSGDPLRRIGEPREIAGIAVYLASRAGAFTTGQNFVIDGG
                     ATIV"
     gene            205..945
                     /locus_tag="AC16VR_05770"
     CDS             205..945
                     /locus_tag="AC16VR_05770"
                     /inference="COORDINATES: protein motif:HMM:NF019032.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HIR6010699.1"
                     /translation="MAADVNSWWPRAILIGAVIAAVLLVAGALGTRIGLWDFQVGFLG
                     LAGGTILAAIGVIVGAVGLIASVKRELPQNRPPIYLGLAISVVILIVMGMQFDKAYSV
                     PPIHNISTDLEDPPAFDQVVALRGEESNPLALNAQEIGPMQREAYPWVVPLVTPTARD
                     EMFSRALAVVDDMGFELVNADPAAGIIEATDTTFWFGFKDDVAIRVRSAEAGTVVDVR
                     SVSRVGLSDLGANAKRIGDILAALGAGA"
     gene            complement(953..1438)
                     /locus_tag="AC16VR_05775"
     CDS             complement(953..1438)
                     /locus_tag="AC16VR_05775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HIR6010700.1"
                     /translation="MKIGVVSDTHNNLRNVGRIVELFNEYGVERVIHTGDITQAKTLD
                     VFARLTAPLWGVYGNNDQERDSLEQAVAAHGFSFTEPPLHLTWHGRKIVVVHDPLEFD
                     GVLNDGHELALHGHTHLYRYEERASQVIFNPGECAGHMAGFNAVGVVDLSDLSAELLR
                     F"
     gene            complement(1529..2674)
                     /locus_tag="AC16VR_05780"
     CDS             complement(1529..2674)
                     /locus_tag="AC16VR_05780"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012336.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR6010701.1"
                     /translation="MTDAYVVGVDMIKFGRFPEKTVPEIGAESALMALDDCGLTIQDM
                     EALYCGNLGQASGMVGQRILQQIGQTGIPVVNVSNACATGATAFREAWASVKAGLYDC
                     VLAVGVEQMGKGLLGGAGGGTGIPKEGLLGSGTMPAVFAEAGMEHTRKYGTTFEQFAK
                     VSVKNHHHSTMNPKAMYQIETPLETVMNAEMISYPNTKLMCSVNVDGSAAAVICSEAK
                     AKELGLMKRAVKVRASVLTSDPWQERDLVMPDVNSCTRKAAAAAYEMAGLGPDDIDLI
                     ELHDCFATAEILHYENLGLCGDGEAGRLIDDGEVALGGRVPVNVSGGLLSKGHPLGAT
                     GIANIYEVCTHLRGEAGQRQVEGARIGLTHVIGLGSACGIHILEKTA"
     gene            complement(2699..3115)
                     /locus_tag="AC16VR_05785"
     CDS             complement(2699..3115)
                     /locus_tag="AC16VR_05785"
                     /inference="COORDINATES: protein motif:HMM:NF013920.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HIR6010702.1"
                     /translation="MSDTPLPVVEYLKIPEDGDPYLEGYKCGSCGATFLGERSVCSKC
                     GARDQMSAVTLPNHGTLYSYSIVHRSFPGIDVPYVSAVVDLDDGTAIKGNLINVEPDP
                     DKIEFGMPVDVVFADALGRKDRDGNSYLSFFFQPSS"
     gene            3258..3698
                     /locus_tag="AC16VR_05790"
     CDS             3258..3698
                     /locus_tag="AC16VR_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR6010703.1"
                     /translation="MKSVKVADYMSTRLITFNRTDSVVEAMAVLLKKKISGAPVVDDE
                     GNLIGILSEKDLMAVVIQDSYYDEGVGIVGDYMREEVDTVDPDSDIYSLADRFHKEGR
                     RRFPVVKNGRLVGQISRRDVLRAAEEFLMHADGLSERKRRKLLT"
     gene            complement(3695..>4087)
                     /gene="gatB"
                     /locus_tag="AC16VR_05795"
     CDS             complement(3695..>4087)
                     /gene="gatB"
                     /locus_tag="AC16VR_05795"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792781.1"
                     /note="allows the formation of correctly charged
                     Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation
                     of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms
                     which lack either or both of asparaginyl-tRNA or
                     glutaminyl-tRNA synthetases; reaction takes place in the
                     presence of glutamine and ATP through an activated
                     phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIR6010704.1"
                     /translation="LSAALNREELGIARSPISPSQLGDLIGRIRDETISGKIAKTVFE
                     GLWAGEGEVDEIIAARGLTQVSDAGEIEAIIAGIVAENPDQVAQFRAGKEKVLGFFVG
                     QVMKATQGKANPKQVNELLRKALAADTG"
BASE COUNT          677 a         1286 c         1381 g          743 t
ORIGIN      
        1 tgggagaatc cggacatcct gaaagcccgc acgtcggggg atccgctccg gcgtatcggc
       61 gagccccggg agatcgcagg catcgccgtg tacctagcgt ccagggccgg cgcttttaca
      121 acggggcaga atttcgtcat cgatggcggc gcgaccatcg tctaggtatg ctcccgcgcg
      181 taaccgaatt cagggaggag tgacatggct gcagacgtta acagctggtg gccgcgggca
      241 atactcatcg gcgcggtgat cgccgccgtc ctgctggtgg cgggcgcgct gggcacccgc
      301 atcgggctgt gggattttca ggtgggcttc ctgggtctcg caggcggaac gatcctggcc
      361 gccatcggcg tcatcgtcgg tgccgtgggc ctcatcgcct cggtgaagcg ggagcttccc
      421 cagaaccgac cgcccatcta cttgggcctc gcgatcagcg ttgtgatact gatcgtcatg
      481 ggcatgcagt tcgacaaggc ctactccgtg ccgccgatcc acaacatctc cacggatctg
      541 gaagacccgc cggccttcga ccaggtggtc gcgctgcgcg gcgaggagtc caacccgctg
      601 gcgctgaacg cccaggagat cgggcccatg cagcgtgaag cctatccgtg ggtcgtgccc
      661 ctggtgaccc cgacagcgcg ggacgagatg ttttcccggg ctctggccgt ggtggacgac
      721 atgggcttcg agctcgtgaa cgccgatcct gccgcgggca tcatcgaggc gacggacacc
      781 accttctggt tcggcttcaa ggacgacgtc gccatccgcg tgcgatcggc cgaggcgggc
      841 accgtggtgg acgttcgcag cgtatcccgg gtgggattga gcgatctggg cgcaaacgcc
      901 aagcggatcg gagacatcct cgcagccctg ggggccgggg cgtaaaccgg ggctagaagc
      961 gcagcagctc ggcgctcaga tccgacaggt caaccacgcc cacggcgttg aacccggcca
     1021 tgtggcccgc gcactcgccg ggattgaaga tgacctggct ggcgcgctcc tcgtagcggt
     1081 acaggtgggt gtggccgtgc agcgccagct cgtgcccgtc gttcagaacg ccgtcgaact
     1141 ccagggggtc gtgcaccacc acgatcttcc ggccgtgcca ggtgagatgt agcggcggct
     1201 ccgtgaatga gaagccgtgg gccgccacgg cctgctcgag ggagtcccgt tcctgatcgt
     1261 tgtttccgta gacgccccac aagggcgcgg tgagccgcgc gaagacgtcc agggtcttgg
     1321 cctgggtgat gtcgccggtg tggatcacgc gctcgacgcc gtactcgttg aagagctcca
     1381 cgattcgacc cacgttgcgc aagttgttgt gggtgtcgct gacgacgccg attttcatgg
     1441 gcttggtggt tggaggcgaa aggccggcct tcagggcggc cccggcccga cggccggggc
     1501 ggccacttgg gacggcgatg gttttggttt aggccgtctt ctcgaggatg tgaatgccgc
     1561 aggcgctgcc cagcccgatc acgtgggtaa gcccgatgcg cgcgccctcg acctgccgtt
     1621 gtccggcctc gccacgcagg tgggtgcaga cctcgtagat gttggcgatc ccggtcgcgc
     1681 ccagggggtg gcccttggag agcagcccgc cggagacgtt caccgggacg cggccgccga
     1741 gcgccacctc gccgtcgtcg atcagccggc ccgcttcacc gtcaccgcaa agacccagat
     1801 tctcgtagtg gaggatctcg gcggtggcga agcagtcgtg gagctcgatg agatcgatgt
     1861 cgtccgggcc gagcccggcc atctcgtagg ctgccgcggc cgccttgcgg gtgcaggagt
     1921 tgacgtccgg catgaccagg tcgcgctcct gccatggatc cgaggtgagc acggaggcgc
     1981 gaaccttcac ggcccgcttc atcagaccca gctccttggc cttcgcctcg gagcagatca
     2041 ccgcagcggc ggagccgtcc acgttcaccg agcacatcag cttggtgttg gggtaggaga
     2101 tcatctcggc gttcatcacc gtctccaggg gcgtctcgat ctggtacatg gccttcgggt
     2161 tcatggtgga gtggtgatgg ttcttcaccg acaccttggc gaactgctcg aaggtggtgc
     2221 cgtacttgcg cgtgtgctcc atgccggctt ccgcgaacac ggccggcatg gtgccggagc
     2281 ccaagaggcc ctccttggga atgccggtgc cgccaccggc cccgcccagc agacccttgc
     2341 ccatctgctc gacgcctacg gcgagaacgc agtcgtaaag cccggccttg acggatgccc
     2401 aggcttccct gaaggcagtg gcgccggtgg cgcaggcgtt ggagacgttg acgacgggta
     2461 tgcccgtctg gccgatctgc tgcaggatgc gctggccgac catgccgctg gcctgaccca
     2521 ggttgccgca gtaaagcgcc tccatgtcct ggatggtgag cccgcaatcg tctaaggcca
     2581 tcagcgcgga ttcggcgccg atctctggga ccgtcttctc ggggaagcgg ccgaacttga
     2641 tcatgtcgac accgactacg tatgcgtctg tcatggagat cctcctattc gaatcgggtc
     2701 aggaactggg ttgaaaaaag aaagacaggt acgagttgcc gtccctgtcc ttgcgcccta
     2761 gcgcgtcggc gaagacgacg tccaccggca tgccgaactc gatcttgtcc gggtcgggct
     2821 cgacgttgat caggttgcct ttgatcgcgg tcccgtcgtc taagtcgacc accgcgctca
     2881 cgtagggcac gtcgatgccc gggaacgagc ggtggacgat ggagtaggaa tacagggtgc
     2941 cgtggttcgg cagcgtcacg gccgacatct ggtcacgggc cccgcacttg gagcacacgc
     3001 tgcgctcgcc caagaacgtg gcgccgcagc tgccgcactt gtaaccctcc aggtagggat
     3061 cgccgtcctc cggaatcttc agatattcca cgacgggcag tggtgtgtcg ctcaaagtgt
     3121 cccccataaa ctcttgtggc ccagccccgg gaggatacta ttgacggccc cctggcgtca
     3181 cctggcgtgg gagggggttt tgcagcaatg tactgaagag ccgcttcagc ggccagcgga
     3241 tcaactgggg agagccggtg aagtcagtca aagtcgcgga ttacatgtcc acgcgtctca
     3301 tcacgttcaa ccgcaccgac agcgtggtgg aggccatggc cgtcctgctg aagaagaaga
     3361 tttccggcgc gccggtggtg gacgacgagg gcaacctcat cggtatcctg tctgagaaag
     3421 acctgatggc ggtggtcatc caggacagct actacgacga gggcgtgggc atcgtgggcg
     3481 actacatgcg cgaggaggtg gataccgtcg acccggattc ggacatctac agccttgcgg
     3541 accggtttca caaagagggc aggcggcgct tccccgtggt caagaacggc cgcctggtcg
     3601 gccagatcag ccgccgggac gtgctgcggg cggcggagga attcctcatg catgccgacg
     3661 ggctgagcga gcggaagcga aggaagcttc tgacctagcc ggtgtcggct gccagggcct
     3721 tgcggaggag ctcgttcacc tgcttggggt tcgccttgcc ctgggtcgcc ttcatgacct
     3781 gcccgacgaa gaaacccagc accttctctt tgcccgcgcg gaactgggcg acctgatccg
     3841 ggttctccgc cacgatcccg gcgatgatgg cctcgatctc gccggcatcg ctcacctgag
     3901 tgaggccgcg ggcagcaatg atctcgtcca cctcgccttc tccggcccac agcccctcga
     3961 acaccgtctt ggcgatcttg ccggagatgg tctcgtcccg gatgcggccg atgaggtcgc
     4021 cgagctgtga aggcgagatg ggcgatcggg caatgccgag ctcttcccgg ttgagcgccg
     4081 cgctgag
//
LOCUS       DBNHQR010000251         1839 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1353020, whole genome shotgun sequence.
ACCESSION   DBNHQR010000251 DBNHQR010000000
VERSION     DBNHQR010000251.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1353020"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="AC16VR_05800"
     CDS             complement(<1..523)
                     /locus_tag="AC16VR_05800"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004882885.1"
                     /note="catalyzes the conversion of carbamoyl phosphate and
                     aspartate to form N-carbamoyl aspartate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HIR6010705.1"
                     /translation="MRKFSRGTPLDFVGSHILSVDQFTREDIEHVFRVADDMQPYADR
                     ERVTRVLQGAILSNMFFEPSTRTRISFGSAFNMLGGEVRETTEVAASSLTKGESLYDT
                     ARVLSGYSDVIVMRHPEAGSVAEFAKASRVPVINAGDGPNEHPSQALLDLYTIRKELR
                     DKGRDLDGLRIALI"
     gene            685..>1839
                     /locus_tag="AC16VR_05805"
     CDS             685..>1839
                     /locus_tag="AC16VR_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HIR6010706.1"
                     /translation="MSDLAHDEPDADIAQVQDAPLPLPFAFARRFGVLITGETGSREQ
                     ALVACKVQPSLTTLAEIKRVVRRELKLKMVGQDEFERLLTATYARDGSAARQMVEDMG
                     DEMDLASLADSVPETEDLLEQEDDAPIIRLINALLAEAIRENASDVHIETFEKDLIVR
                     FRVDGVMREVVKPKRQLAPLLVSRIKVMARLDIAEKRVPQDGRISLRIGGREVDVRVS
                     TMPASNGERVVLRLLDKQAGRLRLENLGMSNEGLELLRDIVHKPHGIFLVTGPTGSGK
                     TTTLYASLAELSTQTINILTVEDPIESNLPGIGQTQVNTKADMTFARGLRAILRQDPD
                     VVMVGEIRDIETAEVAVQASLTGHLVMSTLHTNTAVGAITRLIDMGVEPFL"
BASE COUNT          344 a          590 c          588 g          317 t
ORIGIN      
        1 cgatcagggc tatgcgcagg ccatccaggt cacgcccctt gtcgcgcagt tccttgcgta
       61 tagtatagag gtccagcagc gcctggctgg ggtgctcgtt gggaccgtct ccggcgttga
      121 tgacgggcac ccggctggcc ttggcgaact ccgctactga tccggcctcg ggatggcgca
      181 tcacgatgac gtcgctgtag ccggagagga cgcgggcggt gtcgtaaagg gattccccct
      241 tggtcagtga cgacgcggct acctcagtgg tctcgcgtac ctcgccgcca agcatgttga
      301 acgccgagcc gaacgagatg cgggtccggg tgctcggctc gaaaaacatg ttggagagaa
      361 tggcgccctg caggacccgg gtgacccgtt cccgatccgc ataaggctgc atgtcgtccg
      421 cgacccggaa aacgtgctcg atgtcctcac gagtgaactg gtccaccgac agtatatggc
      481 tgccaacgaa gtccaacggc gtcccccggc tgaatttgcg caagtctaca cctaccgcgg
      541 cgtcacggga aaacgagccg ccgttcacgc tttgtgatga ccgtcacgga agcaagggca
      601 gcgccacacg ggttggaatc tttgggttta gcggtgcgtg cctgggttgc tatcattggg
      661 ctgccccagt acgagtaccg ccagatgagc gacttggcac atgacgagcc ggacgccgac
      721 atagctcagg tgcaggacgc gccgttgccc ctgcctttcg cctttgcccg ccgattcggc
      781 gttctgatca cgggagaaac cggctcccgg gagcaggccc tggtagcctg caaggtccag
      841 ccgagcctca ccaccttggc cgagatcaag cgcgtggtgc ggcgcgagct gaagctgaag
      901 atggtgggtc aggacgagtt cgaaagactg ctcacggcga cctacgcccg ggacggctcc
      961 gccgcccgcc agatggtcga ggacatgggc gacgagatgg atctcgcaag ccttgccgac
     1021 tcggtcccgg agaccgaaga ccttctcgag caggaggacg atgcgcccat catccggctc
     1081 atcaacgcgc tgctggccga ggccattcgc gaaaacgctt ccgacgttca catcgagacg
     1141 ttcgagaagg acctcatcgt ccggttccgg gtcgacgggg tgatgcggga agtggtcaaa
     1201 cccaagcgcc agctggcgcc tctgctggtc tcccggatca aggtcatggc ccgcctggac
     1261 atcgccgaga agcgggttcc tcaggacggc cggatctccc tgcgcatagg cggccgggaa
     1321 gtcgacgtgc gggtttccac catgccggcc agcaacggtg aacgggtggt gcttcggctg
     1381 ctggacaagc aggcaggacg gttgaggctg gaaaatctgg gcatgtcgaa cgaagggctc
     1441 gagttgctgc gcgacatcgt tcacaagcct cacggcatct tcctggtcac cggccccacc
     1501 gggtcgggta agaccaccac gctctacgcc tccctggccg agctgagcac tcagaccatc
     1561 aacatcctga cggtcgagga ccccatcgag tccaacctgc ccggcatcgg ccagacccag
     1621 gtgaatacca aggcggacat gaccttcgcc cgcggccttc gcgccattct gcgtcaggac
     1681 cccgacgtgg tgatggttgg cgagatccgg gacatcgaga ccgccgaggt cgccgtccag
     1741 gcaagcctca cggggcacct cgtgatgtcc accctccaca ccaacacggc cgtgggagcc
     1801 attacccgcc tgatcgacat gggcgtcgag cccttcctg
//
LOCUS       DBNHQR010000252         4240 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_213963, whole genome shotgun sequence.
ACCESSION   DBNHQR010000252 DBNHQR010000000
VERSION     DBNHQR010000252.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4240)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_213963"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            443..1045
                     /locus_tag="AC16VR_05810"
     CDS             443..1045
                     /locus_tag="AC16VR_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010707.1"
                     /translation="MTWTQILATYLVVGGTVLLFTPVRPTLARHLNLYEQRHISRLAF
                     VVYAGIVVASDLLLWPFLAAKHRPSRRRVNKPEHAVLDPELIEGQKILRDSLGQMGDD
                     GVNNDELPAATGDFGREASNPIPCNTVFGSTAYLARLRFSDGAKILFDRLGSVSSDAS
                     RYPVDCYAIKHPDGRGLGVFYISPYHKRNSRKAPEGFSID"
     gene            complement(1052..3481)
                     /locus_tag="AC16VR_05815"
     CDS             complement(1052..3481)
                     /locus_tag="AC16VR_05815"
                     /inference="COORDINATES: protein motif:HMM:NF039587.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HIR6010708.1"
                     /translation="MTRCRVLMVALALIVAGRSLAESTAGAAQSRSAELNTGEVVDIP
                     YRFHEQASIRVDGRLDDAAWADATVIRDFIVIDPDTMEKPRWETKLMLIYTERGIYAG
                     YDLEQPRDMIVSVLSPRDTGTNFGDFVGLVLDTSGDGKYGYWMSLAASGVKTDGTLIP
                     EAQFNRDWDGVWYGETALTEKGWSAEFFVPWSQLAMPKEAGTRRMSLFASRRSVAIGE
                     RWGVPALPFTITKWIQYMRPLEMEGVDPRQQWSVIPYTSVTQDQVEKETDFRAGAEVF
                     WRPSSNFQVSASVLPDFGNVESDAVIVNLSALETFFPEKRLFFLEGRDIFLTSPRADA
                     SKNFFPVTVVNTRRIGGSPRPPDVPEGVAIPSRQLDQPVELYGAVKATGQLGSLRYGL
                     LGAMEDDVTFKVDDLRFEQSGSEYGVARLLWEDVSYGSYRALGTISTLTAHPEEDAVV
                     HGIDYHYMTGNGSWKLDGQVLYSDKDSVGDGWGTFADAVYTPRRGLKVNFEFEYYDDK
                     LDINDLGFLQRNDITRGGIGVDYTNPSVGWARQLNLSGLSIYEVNGDGDVTRQGASAS
                     TNVLLNNLGRVRASALFFGRRTDDLNSFGNGSFVIPSRWEGNVDYFSDPSRSLSYRVG
                     FDLDEETVHGMAIKARLGATWRPSQRMKLDLFTEYVRRDGWLLHQDAPEDFDGPNNHF
                     TTFDTREWRPTLGIDFFFTAKQQIRLSAQWVGIQAREQRFFQIPENEGELDEVDKPWE
                     QSDDFTISNLNVQLRYRWELAPLSDLFVVYTLNGAQGPPRASFADLLESSFSDPVFEK
                     IVVKLRYRFGS"
     gene            complement(3492..3677)
                     /locus_tag="AC16VR_05820"
     CDS             complement(3492..3677)
                     /locus_tag="AC16VR_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010709.1"
                     /translation="MADVLPFKKRSLKEKAKGRTLCARGFHKWVVDKQQRFDVKQGGL
                     VTVHRCQRCGATKNAAI"
     gene            complement(3802..4158)
                     /locus_tag="AC16VR_05825"
     CDS             complement(3802..4158)
                     /locus_tag="AC16VR_05825"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6010710.1"
                     /translation="MVSIAVVDDDVAILDAMTLLAESRGWLAKVFASGEAFLDAFEHD
                     RDIDCLVLDPHLQGIGGDDVALALADTGVPIVGLTARPESPVTQSILESGAAAILTKP
                     VRPAELVSAIENLLLN"
BASE COUNT          817 a         1392 c         1261 g          770 t
ORIGIN      
        1 caaccgcctg gtggcgcgcg ccgccgagct cggcttccca accgagaatc tcatattcgt
       61 cgatcactcc ggcgcgaatg cccactgaaa gacgggcggt aagggcgacc cgccgggctg
      121 atgacccaat ctggccatgg ccatgaccgg ctgttaggca tctttctaca aatcgctcac
      181 aaaacggcgt tccttcgacg ccgcgccacg tctaacctaa ttgaatgatc ggggggccag
      241 aatacgacac accagcgcac cggcgttggg ataggggcag cgaaagcgac ggcccgatgc
      301 ccacccgcca tcggcgctca cacggcggtc ccgacaggaa cccaagcacg ctgtacgacc
      361 cccgccaact ggctttgctg cctgaactcc agggcgatct cttcgaggag ctgtccagtt
      421 ccatggtaag cgggcgatga cgatgacctg gacgcagatc ctcgcaacct acctcgtcgt
      481 cggcggcacc gttctcctat tcaccccagt ccggcccacg ctcgcgcgtc atctgaacct
      541 atacgagcag cgccacattt cccgcctggc gttcgtcgtc tatgccggta tcgtcgtggc
      601 cagcgacctg ctcctctggc cgtttctcgc cgccaaacat cgcccctcgc gccggcgggt
      661 caacaagccg gagcacgcgg tcctggaccc ggagctgatt gaggggcaga aaattctgcg
      721 cgactcactc gggcagatgg gcgacgacgg cgtcaacaat gacgagcttc cggcagctac
      781 gggggatttc gggcgcgaag catcgaaccc gattccgtgc aacacggttt tcggcagcac
      841 cgcgtacctg gcaaggctga gattctcaga cggtgcgaag atactcttcg accgcctggg
      901 ctctgtatcc tcggacgcct ctcggtaccc cgtcgactgc tacgccatca agcatcccga
      961 cggccgcggc cttggcgttt tctacatttc cccctatcac aagcgcaact cacgaaaagc
     1021 gcccgagggt ttctcaatcg actgagcggc gtcagcttcc gaaccgatag cgaagcttga
     1081 ccacgatctt ctcgaacacc gggtccgaga acgatgactc cagcagatcc gcaaagctcg
     1141 ccctcggcgg accctgcgcc ccgttcaacg tgtagaccac gaacaggtcc gacaacgggg
     1201 cgagctccca gcgataacgc agctgcacat tcaggttgga tatcgtgaaa tcgtcgctct
     1261 gctcccaagg tttgtcgacc tcgtcgagct cgccttcgtt ctcaggaatc tggaagaacc
     1321 tctgctcacg ggcctgaatg cccacccact gcgcggagag ccggatctgc tgctttgccg
     1381 tgaagaagaa gtcgatgccc aacgtggggc gccactcccg ggtatcgaac gtggtgaagt
     1441 ggttgttggg accgtcgaag tcctccggag cgtcctggtg cagcagccaa ccgtcccgac
     1501 gcacgtactc agtgaacaga tccaacttca ttcgctggga cggccgccag gtcgcgccga
     1561 gcctggcctt gatcgccatt ccatgaaccg tctcctcgtc cagatcgaag ccgacccgat
     1621 agctgagcga acgggaggga tcggagaagt aatcgacgtt gccttcccac cggctgggga
     1681 tgacgaagga gccgttgcca aaactgttga gatcgtcggt ccggcgaccg aagaacagcg
     1741 cggacgcccg cacacggccc agattgttca gcagcacgtt tgtcgaagca gaagccccct
     1801 gccgggtgac gtccccgtcg ccgttcacct cgtaaatcga cagcccgctc aggttgagct
     1861 gccgggccca gccgacgctg gggttggtgt agtcgacgcc gatgccgccc cgggtgatgt
     1921 cgttgcgctg cagaaagccc agatcgttga tatccagctt gtcgtcgtag tactcgaatt
     1981 cgaagttgac cttgaggcca cgacgcgggg tatacaccgc atccgcgaac gtgccccatc
     2041 cgtcaccgac gctgtccttg tccgagtaaa gcacctggcc gtcgagcttc cacgagccgt
     2101 ttcccgtcat gtagtgatag tcgatcccat gcacgacggc gtcctcctcg ggatgggccg
     2161 tgagagtgga gatggtgccc agagcccggt aacttccgta ggaaacgtct tcccagagca
     2221 ggcgggcaac cccgtattcg cttccggact gctcgaacct caggtcgtct accttgaacg
     2281 tcacgtcgtc ttccatggcg ccgagcagac cgtagcgcaa tgagcccaac tggccggtgg
     2341 ccttgaccgc tccgtaaagt tcgacgggct ggtcgagctg cctggacggg atcgcaaccc
     2401 cctccggcac gtcgggcggc cgcggcgaac cgccgatgcg ccgcgtgttc accaccgtga
     2461 ccggaaaaaa gttcttagac gcgtcggctc tcggcgaggt gagaaaaata tcgcggccct
     2521 ccaggaagaa cagccgcttc tcggggaaaa aagtctccag agccgaaaga ttcacgatga
     2581 ccgcgtccga ctcaacgttg ccgaaatcgg gcaaaacgga tgcggaaacc tggaaattgc
     2641 tggacggccg ccagaacacc tcggcgccgg cccggaaatc ggtctccttc tccacctgat
     2701 cctgggtcac cgaggtgtaa ggaatcacgc tccactgctg gcgcgggtcc acgccttcca
     2761 tctccagcgg ccgcatgtac tggatccact tggtgatggt gaaaggcagg gcgggcacgc
     2821 cccagcgctc gccgattgcc acggagcgac gcgacgcaaa gagcgacatg cgccgggtgc
     2881 ccgcttcctt cggcatcgcc agctgcgacc agggaacgaa gaactccgcc gaccacccct
     2941 tttcggtcag ggcggtttct ccgtaccaca ctccgtccca gtcacggttg aactgagcct
     3001 cgggaatgag ggtcccgtcg gttttgaccc cgctggcggc aagggacatc cagtatccgt
     3061 acttgccgtc gccggatgtg tcgagaacca ggccgacgaa gtcaccgaag ttcgtccccg
     3121 tgtcccgcgg cgaaagaacc gagacgatca tgtcacgcgg ctgctccaga tcgtagccgg
     3181 cgtagatgcc gcgctcggtg tagatgagca tcaacttcgt ttcccagcgg ggcttttcca
     3241 tggtgtccgg gtcgatgacg atgaagtcgc ggatgaccgt cgcatccgcc caggccgcgt
     3301 cgtcgaggcg gccgtccacc cggatggacg cctgttcgtg gaacctgtaa ggaatgtcga
     3361 cgacctcacc ggtattgagc tcggcagacc tggattgcgc agcgccggct gtggattcgg
     3421 ccaggctacg tcccgcaacg atcagggcta aagccaccat gagaacgcgg caccgggtca
     3481 tgctggggcg ctcagatggc cgcattcttg gtcgcgccgc agcgctggca gcgatggacg
     3541 gtcaccagcc cgccctgctt gacgtcgaag cgctgctgct tgtcgaccac ccatttgtgg
     3601 aatcctctcg cacacaaggt ccggcctttg gctttctcct tgaggctgcg cttcttgaac
     3661 ggcaggacgt cagccatcgc ggctcccgga ttctgcaaaa gttgaccggc ccgacacacc
     3721 ggcatggggt tcgccggggg cgaattgttg cagctttgca ggttcaacgc tcgccgatcg
     3781 ttgcccggcg cagcaaggcc ctcaattcag caacaggttc tcgatggcac ttaccagctc
     3841 ggcaggcctg accggcttgg tgaggatggc tgccgcccca ctctcgagaa tcgattgagt
     3901 cacggggctc tccggtcgag cggtcaggcc gacgatgggt acgcccgtat ccgccagcgc
     3961 cagggcgacg tcgtcgccgc cgataccctg caggtggggg tcgaggacca ggcagtcgat
     4021 gtcccggtcg tgctcgaacg cgtcgagaaa agcctctccg ctggcaaaga ctttggccag
     4081 ccagccccga ctctcagcga gcagggtcat ggcatccaga atggccacat catcgtctac
     4141 caccgcaatg gaaaccatcg cccaccgtcc gcatttgcct tttagcgagg ttaaggcggg
     4201 cagctgtggc ctcaattcgg gaatctacct agccgggccc
//
LOCUS       DBNHQR010000253         3519 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_466899, whole genome shotgun sequence.
ACCESSION   DBNHQR010000253 DBNHQR010000000
VERSION     DBNHQR010000253.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_466899"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="AC16VR_05830"
     CDS             complement(<1..278)
                     /locus_tag="AC16VR_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010711.1"
                     /translation="MKAGIQTRLLITTALVLGTSLALAGVVLDRSFQASVRAGAEEQL
                     RLIIYSLMGAVVEGNDDIRLNPDLPEPRLNQPGSGLYAVVDRGDGGDR"
     gene            complement(275..946)
                     /locus_tag="AC16VR_05835"
     CDS             complement(275..946)
                     /locus_tag="AC16VR_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6010712.1"
                     /translation="MRILIVEDEAILRDQLVSRLKSAGYVVDAAQDGEEGLYYGQEYD
                     YDAAIVDLGLPKIDGIDLIERLRGEERRFPVLVLTARGNWQDKVEGLEAGADDYLTKP
                     FQLEELLARLNALIRRAGGYASPVINEGALSVDTAKKEVRVEDAVVDLTAYEYRVLEY
                     LLLNAARVVSKAELTDHLYEQDFDRDSNVIEVFVGRLRKKLDPVNPIRTVRGQGYRFN
                     TEGSA"
     gene            complement(977..1300)
                     /locus_tag="AC16VR_05840"
     CDS             complement(977..1300)
                     /locus_tag="AC16VR_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HIR6010713.1"
                     /translation="MVKGRSLMNLRCRGRWLALAVMMIPAIAGAAPSDVFEKALEPNR
                     FAPPPGISMEQAADMVRRDTGGRVLSATPTQRGDRRGYEIRVLLDGKRVKKVFVDSQA
                     KPPGQ"
     gene            complement(1350..1877)
                     /locus_tag="AC16VR_05845"
     CDS             complement(1350..1877)
                     /locus_tag="AC16VR_05845"
                     /inference="COORDINATES: protein motif:HMM:NF017269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HIR6010714.1"
                     /translation="MYASHTAKTLLVVALVAPALAVAGTSYDRARVVSVEPLYETVSY
                     EVPREECRMEEVRHTRSGSHRSYTAPIVGAIVGGAIGNAVGHKKRNKQIGTVVGAVLG
                     GSIGADIARRGHHHGHGPEYGLERVCRTVSDWHEEQRLLGYRVTYRYAGETYITQLDE
                     APGSSLPVRVQVTPV"
     gene            complement(1958..2839)
                     /locus_tag="AC16VR_05850"
     CDS             complement(1958..2839)
                     /locus_tag="AC16VR_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010715.1"
                     /translation="MAGLTLDDLAQALLAGSEPWTDPGALAARLDPDIPEDWRNLAAV
                     VVAAGATGTIGVGGGQGAGKSTLAAAMVEASRLAGRKAQACSLDDFYLTRQERLALAR
                     TVHPLFVTRGVPGTHDVDLALSVLRGLEGVGVTRVPVFDKGRDDRAPGPEWRAVEGPV
                     ESIVFEGWCLGAPPEPAERLIEPMNSLEREEDANRTWRSHVNDVLGESYQRLWSAMDR
                     LLYLQVPDMAAVHDWRAQQELALPPSRRMSGGQLDRFLAHYERLTRWMLETLPERADV
                     VVELGRDHRISGVRRNS"
     gene            2972..3298
                     /locus_tag="AC16VR_05855"
     CDS             2972..3298
                     /locus_tag="AC16VR_05855"
                     /inference="COORDINATES: protein motif:HMM:NF019301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HIR6010716.1"
                     /translation="MTVSPPLPLGSTASWFLASWTEFLGGILLLFGLGTRLVAKAKDI
                     LREHGDYRYLNGSGSIVKLNGGIEFAAIYIAMLLALLVIGGGRYLSVDYDVGLLGRTR
                     ASAQRE"
     gene            3336..>3519
                     /locus_tag="AC16VR_05860"
     CDS             3336..>3519
                     /locus_tag="AC16VR_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010717.1"
                     /translation="MLKLIPLALLSLVLGSCANEPARPPAPDPGAPVVIAKSPNDERS
                     YRYLVLDNELRVLLVSD"
BASE COUNT          604 a         1200 c         1086 g          629 t
ORIGIN      
        1 cagcggtcgc cgccgtcacc ccggtccacc acggcgtaaa gaccactgcc tggctggttc
       61 aggcgcggct ccggcagatc cgggttgaga cggatgtcgt cgttaccctc caccaccgcg
      121 cccatcaggg agtagatgat caggcgcagc tgctcttcgg cacctgctct gacgctggcc
      181 tgaaaggagc ggtcgagcac gacgcccgcg agcgcgagcg acgtgccgag caccagggca
      241 gtggtgatga gcaggcgggt ctggatgcct gccttcaagc agatccttcc gtgttgaacc
      301 ggtacccctg gccgcgaacg gtgcggatgg ggttcacggg atcgagcttt ttgcgcaggc
      361 gaccgacgaa gacctcgatg acgttgctgt cgcgatcgaa gtcctgctcg tagagatggt
      421 cggtgagctc ggccttggag accacccgtg cggcgttgag cagcaggtac tcgaggaccc
      481 ggtactcgta ggcagtgaga tccacgacgg cgtcctccac gcggacctct ttcttggccg
      541 tatccacgct gagcgcgccc tcgttgatca ccggcgaggc gtagccgccc gcccttctga
      601 tcagagcgtt caggcgggcc agcagctctt cgagctgaaa tggcttggtc aggtagtcgt
      661 cggctccggc ctccagcccc tccaccttgt cctgccagtt gccgcgcgcc gtcagcacca
      721 gaaccgggaa acgccgttcc tcgcccctga gccgctcgat gaggtcgatc ccgtcgatct
      781 tcggcaggcc tagatcgacg atggcggcgt cgtagtcgta ctcctggccg taataaaggc
      841 cctcctcgcc gtcttgtgcc gcgtccacca catatcccgc gctcttcagc cgcgaaacca
      901 gctggtcgcg aagtatcgct tcgtcttcga ctatcaggat gcgcatgacc gattcgctac
      961 ctcacaaggc tccggatcat tgtccggggg gcttcgcctg ggagtcgacg aacaccttct
     1021 tgacccgttt cccgtccagg agcacgcgaa tttcgtagcc gcgccgatcg ccccgctggg
     1081 tgggcgtggc cgacaatacc cggcctcccg tgtcccggcg caccatgtcc gcggcctgtt
     1141 ccatggagat gcccggcggc ggggcaaacc ggttgggctc cagggctttc tcgaacacgt
     1201 cggacggggc agcaccggcg atcgcgggaa tcatcatcac cgcgagcgca agccatctgc
     1261 cacggcagcg caggttcatc agagagcggc ctttgaccat agcgtgctca ttgtacccgc
     1321 aatttccatc ccttctaacg ttgccccggt cacaccggcg tgacctgcac gcgcacggga
     1381 aggctcgaac ccggcgcctc gtccagttgc gtgatgtagg tctcccccgc gtagcggtag
     1441 gtgacacggt aaccgagcaa ccgctgctcc tcgtgccagt cgctgacggt ccggcaaacc
     1501 cgctccagac cgtattccgg tccgtgcccg tggtggtgac cgcgccttgc gatgtccgcg
     1561 ccgatactgc cgccgagcac ggctcccacc acggtgccga tctgcttgtt gcgcttcttg
     1621 tggcccacgg cgttgccgat ggcgccgcca acgatggcgc cgacgatggg cgccgtgtac
     1681 gaacgatgcg acccgcttcg ggtgtgccgc acttcctcca tccggcactc ctcgcgcggg
     1741 acctcgtagg agacggtttc gtaaaggggc tcgacggaaa ccaccctggc ccgatcgtag
     1801 gacgtgcccg ccactgccag tgccggcgcc accagggcga cgaccagcag agttttcgct
     1861 gtgtgggatg catacatcgt cattctccat tcgactgcaa tgacggccac cagattaagg
     1921 accgacgact gaacggatca tgaatacggc ctacgggtca ggaattcctg cgaacgcccg
     1981 agatccgatg gtcgcgaccg agctcgacga ccacgtctgc ccgttcgggc agggtttcga
     2041 gcatccagcg cgtcagccgc tcgtagtggg caaggaaccg gtcgagctga ccgcccgaca
     2101 tgcgcctgga cgggggcagc gccagctcct gctgtgcgcg ccagtcgtgc acggcggcca
     2161 tgtccggaac ctggaggtag agcaggcgat ccatcgcgct ccaaagccgt tgatagctct
     2221 cgcccagaac atcgttcacg tgggagcgcc aggtccggtt cgcatcctct tcgcgctcga
     2281 gcgagttcat cggctcgatg agacgttcgg caggctcggg cggggcgccc aggcaccagc
     2341 cctcgaagac gatgctctct accgggccct cgaccgcacg ccactccggg ccgggcgcac
     2401 gatcgtcccg gcctttgtcg aatacgggca ccctggtgac gccgacaccc tcgagcccgc
     2461 gcagaacgga cagcgccaga tcgacgtcat gggtaccggg aacgccccgg gtcacgaaga
     2521 gcggatgaac ggttcgtgcc agagccagac gctcttgcct ggtgagatag aaatcatcga
     2581 gcgagcaggc ctgcgccttt cgtccggcca ggcggctggc ttcgaccatc gccgcagcca
     2641 gagtcgactt gcccgctccc tggcccccgc cgacgccgat ggtgcccgtc gctcccgcag
     2701 cgacgacgac ggccgccaga ttgcgccagt cttcgggaat gtcgggatcg aggcgggccg
     2761 ccagcgcccc gggatccgtc catggctcgg aacctgcgag cagcgcctgg gccaggtcgt
     2821 cgagtgtcag acccgccatg gttttccatc acctgacggt ttgtgcatca tggaccgccc
     2881 tccggggaac cctgctccgc cttcgagtct aattctgaaa aacatgggag aagcctcatc
     2941 cagccaatca tagcaaccgt agctgactgg catgaccgtc tcgccaccat tgccgctcgg
     3001 ctcgacggct tcgtggttcc tggcttcctg gaccgagttc cttggaggca tcctgctgct
     3061 gttcggactt ggcacccggc tggtcgccaa agcaaaggac atcctgcgag aacacggcga
     3121 ctatcggtat ctcaacggca gcggcagcat cgtcaagctg aacggcggca tcgaatttgc
     3181 cgccatctac atcgccatgc tcctggcgtt gctggtgatc ggaggtggcc gttatctcag
     3241 cgtcgactac gatgtcgggc tgctcgggag aactcgcgcg tccgcacaac gggaatgatc
     3301 gccgcccgga ccgctcaaca actggaaacc gaaacgtgct gaaactcata ccgctcgcgc
     3361 tgctttcact ggtgctggga tcgtgcgcca acgaaccggc gcgcccgccg gcccccgatc
     3421 ccggggctcc ggtcgtcatc gccaagagtc cgaacgacga aaggagctac cgctacctcg
     3481 ttctcgacaa cgaactgcgg gtgctgctcg tatccgacc
//
LOCUS       DBNHQR010000254         3114 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_340858, whole genome shotgun sequence.
ACCESSION   DBNHQR010000254 DBNHQR010000000
VERSION     DBNHQR010000254.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_340858"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="AC16VR_05865"
     CDS             complement(<1..275)
                     /locus_tag="AC16VR_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HIR6010718.1"
                     /translation="MNRSLRGRVAVVGVGETMYYKAGQSPDAEFKLALKAILAACEDA
                     GIDPKDVDGFASYSNDRSDPSRLAAALGCRELRFANMQWGGGGGGGSG"
     gene            complement(300..926)
                     /gene="amrA"
                     /locus_tag="AC16VR_05870"
     CDS             complement(300..926)
                     /gene="amrA"
                     /locus_tag="AC16VR_05870"
                     /inference="COORDINATES: protein motif:HMM:TIGR04335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HIR6010719.1"
                     /translation="MPSTDYGPDARQALLGIARDAIRIGLERHGVADPMSVVDVPSLL
                     PELLEPRATFVTLTQAGSLRGCTGSLEALRPLALDVVENACHSAFRDPRFPPVQAGEL
                     NRLRIELSVLSPLERMRVASEGELLERLEPSMGLVIEEGGRRGTFLPKVWESLPDPRV
                     FLDELKAKAGFPAGHWSDATEVFYYRTQTFAEPVAAFGSVVSHGGDSV"
     gene            complement(913..1698)
                     /gene="amrB"
                     /locus_tag="AC16VR_05875"
     CDS             complement(913..1698)
                     /gene="amrB"
                     /locus_tag="AC16VR_05875"
                     /inference="COORDINATES: protein motif:HMM:NF013992.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HIR6010720.1"
                     /translation="MVSRSPAVADLFYPGDPAELAGTVRAYLDRADAPTVALRPKALI
                     VPHAGYAYSGPVAATAYRTLEPFRHEVERVILMGPAHRVYLEGLAVPETDAFAVPTGN
                     VPVDVEAVARLLELPGVLASDQAHAAEHCLEVHLPFLQPVLDRFSIVPVVVGACSSEL
                     VADVLDLLWGGDETLIVVSSDLSHYHPYEVAQALDAATAGEILARACNLTGEQACGAV
                     AINGLMLAATGHDLQVRELDLRNSGDTAGDRDRVVGYGAFALD"
     gene            1759..2835
                     /gene="amrS"
                     /locus_tag="AC16VR_05880"
     CDS             1759..2835
                     /gene="amrS"
                     /locus_tag="AC16VR_05880"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HIR6010721.1"
                     /translation="MDQTIVPTRYWHTLDDGRTQCDVCPRHCRLREGQRGLCYVRQCL
                     DGEVKLVSYGRSSGFCVDPIEKKPLNHFYPGSSVLSFGTAGCNLACKFCQNWDMSKSR
                     EMDTLADRAMPERLAEAAVDLACRSVAFTYNDPVIFLEYAVDVADACREVGVKSVAVT
                     AGYVDPEPRREFFSAVDAANVDLKAFSERFYHKICGGHLAPVLETLEFIRHETNTWLE
                     LTTLLIPGENDSDEELDEMTGWVVEHLGPDVPMHFTAFHPDWKMRDVPSTPPGTLTRA
                     RSIARRNGVHFAYTGNVHDPAGSSTYCSACDALLIERDWYQLGRWGLDGAGRCSACGE
                     PLAGHLEGPPGQFGSRRIPVRLDQ"
     gene            2926..>3114
                     /locus_tag="AC16VR_05885"
     CDS             2926..>3114
                     /locus_tag="AC16VR_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010722.1"
                     /translation="MKNLFFAMIATLLLAGCATTEQVEALQNRVSALENQIGSANDAA
                     GDAASAASRAASDAAAAQS"
BASE COUNT          581 a         1060 c          989 g          484 t
ORIGIN      
        1 ccggagccgc cccctccgcc accgccccac tgcatgttgg caaagcggag ttcacgacaa
       61 cccagcgctg cggccagccg cgacgggtcg ctgcggtcgt tgctgtagga agcgaaacca
      121 tcgacgtcct tcgggtcgat gcctgcatcc tcgcaggccg cgaggatcgc cttgagcgcg
      181 agcttgaact cggcatccgg cgattggccg gccttgtagt acatggtctc gcccacgccg
      241 acgacggcga cccggcccct gagcgagcga ttcatctggt ttcccccatc ccggggaatc
      301 tacaccgaat caccgccatg cgacaccacg gaaccgaacg ctgccaccgg ctcggcaaac
      361 gtctgggttc ggtagtagaa aacctctgtg gcatccgacc agtggccagc cgggaagccg
      421 gctttggcct tgagctcgtc caggaaaacg cgtggatccg gcaggctttc ccagaccttg
      481 ggcagaaagg taccgcgccg gccgccttcc tcgatcacca gacccatgga cggctcgaga
      541 cgctccagga gctccccctc gctggctacc cgcatccgct ccagggggga gagaaccgaa
      601 agctcgatgc gcagacggtt cagctcgcct gcctgaaccg gagggaagcg cgggtcgcga
      661 aatgcggagt ggcaggcgtt ttccaccaca tcgagcgcga gcggcctcaa cgcctccagc
      721 gaacccgtgc aaccgcgcaa cgacccggct tgggtcaggg tgacgaacgt cgctcgaggc
      781 tcgagaagct cgggaagcag gctgggaacg tccaccacgg acatcgggtc cgcgacgcca
      841 tggcgttcga gaccaatccg aatggcgtcc cgcgcgatgc ccagcagggc ctgtcgcgcg
      901 tcggggccgt aatcagtcga gggcaaacgc gccataaccc accacccgat cacggtcgcc
      961 ggccgtatcg cccgaattgc gaaggtcgag ctcccgcacc tgaaggtcgt gaccggtagc
     1021 cgccagcatc aaaccgttga tggcgaccgc tccgcacgcc tgctcgccgg tcaggttgca
     1081 ggcgcgagcg agaatctcgc ctgccgtcgc cgcatcgagc gcctgagcca cctcgtaggg
     1141 atgatagtgg gacagatcgg aactcacgac gatgagggtt tcgtctccgc cccagagcag
     1201 gtcgagcacg tcggccacca gctcgctcga gcacgcgccg acgaccacgg gaacgatgga
     1261 gaaacgatca agcaccggct ggaggaaggg caggtgcacc tccaggcagt gctcggcggc
     1321 gtgggcctgg tcagaggcca gcactccggg gagctcgagc agtcgggcca cggcttcgac
     1381 gtccaccggc acgttacccg tcgggaccgc aaacgcatca gtctcaggga ctgccaggcc
     1441 ttccagatac acccgatggg ccgggcccat gaggatcaca cgttccacct cgtgccgaaa
     1501 aggctcgagg gtccgatacg ccgtcgccgc caccggcccg gagtatgcgt agccggcgtg
     1561 aggcacgatc agcgccttcg gtcgcagggc gaccgtgggc gcatccgcgc ggtccaggta
     1621 cgcccgcacc gtcccggcca gctcggccgg atctccggga tagaacaggt ccgctacggc
     1681 gggcgatctg ctgaccatgg cattcggaag attgttaagg cgcttggtat atgggggtac
     1741 cgaccccata aagcaaccat ggatcagaca atcgtgccca cgcgctactg gcatacgctg
     1801 gacgatggcc gcactcagtg cgacgtttgt ccgcgccact gccgcctgcg cgagggccag
     1861 cggggactgt gctacgtgcg ccagtgtctc gacggtgagg tgaagctcgt cagctacggc
     1921 agatcgtcgg ggttctgcgt ggaccccatc gagaagaaac cgctgaacca cttctacccc
     1981 ggcagcagcg tgctctcctt cggcaccgcg ggctgcaacc tcgcctgcaa gttctgccag
     2041 aactgggaca tgagcaagtc ccgcgagatg gacaccctgg ccgatcgagc catgccggag
     2101 cggctggccg aagccgcggt ggatctggcg tgccgaagcg tggctttcac ctacaacgac
     2161 ccggtcatct tcctggagta cgccgtcgac gtcgccgacg cctgtcgcga agtcggcgtg
     2221 aagtccgtgg ccgtgacggc cggatacgtg gaccccgagc cccggcgcga gtttttctcc
     2281 gcggtggacg ccgccaacgt cgacctcaag gcgttcagcg agcgcttcta ccacaagatc
     2341 tgcggcggcc acctcgcgcc ggttctggag acgctggaat tcatccgcca cgagaccaac
     2401 acctggctcg agctgaccac gctgttgata ccgggcgaga acgactccga cgaggagctc
     2461 gacgagatga cgggctgggt cgtcgagcac ttgggtcccg acgtgcccat gcacttcacc
     2521 gcctttcacc cggactggaa gatgcgcgac gtgcccagca cgcccccagg caccctgacc
     2581 cgggctcgca gcattgccag gcgcaacggg gtgcacttcg cctacaccgg caacgtgcac
     2641 gatcctgccg gctccagcac ctactgctcg gcctgcgatg ccctgctcat cgagcgcgac
     2701 tggtatcagc tgggccgctg gggactggac ggggccggac gctgcagcgc ctgcggcgag
     2761 ccgctggccg gacatctcga agggcctccg ggccagttcg gaagccggcg catcccggtg
     2821 cgactcgatc aatgagcata atttcagcgc aatcaactga tgacaaagcc gctgactatg
     2881 gtagggttcg cttccgagaa aaacctggct gagtgaggcc cccaagtgaa aaatcttttc
     2941 tttgccatga tcgccaccct gctgctcgca ggctgcgcaa ctaccgaaca ggtcgaggct
     3001 ctccagaacc gcgtcagcgc gctggagaac cagattggct ccgctaacga cgctgccgga
     3061 gatgccgctt ctgctgcttc ccgagcggcg agcgacgccg cggctgctca gtcc
//
LOCUS       DBNHQR010000255         3358 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_87456, whole genome shotgun sequence.
ACCESSION   DBNHQR010000255 DBNHQR010000000
VERSION     DBNHQR010000255.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_87456"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..440)
                     /locus_tag="AC16VR_05890"
     CDS             complement(144..440)
                     /locus_tag="AC16VR_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HIR6010723.1"
                     /translation="MDKDQENAIEAAVFRRLLEHLDEHKDVQNIDLMNLAGFCRNCLS
                     KWYRAACEDAGVAMDYEAARERVYGMPYADWKSRYQAEATPEQLAGFESGRQQG"
     gene            557..1336
                     /locus_tag="AC16VR_05895"
     CDS             557..1336
                     /locus_tag="AC16VR_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HIR6010724.1"
                     /translation="MELLTNPEIWAAFVTLTILEIVLGIDNIIFISILTSRLPPEQRA
                     RGRVLGLGLAMLMRILLLLSLAGIMRLTTDLFEVMGQGISGRDLILIFGGAFLMAKAT
                     REVHNALEGEAHEGQGAGAPTFAGVLLQIALIDMVFSLDSVITAVGLVDEVPVMVAAV
                     ISAVLVMMVAAGPISDFVEDHPTVKMLALAFLLLIGLTLMGEGIEIHTPKGYIYFAMA
                     FSFGVEILNIRAKKRRKDHVMLRKTQLADLVASRSEKGRPD"
     gene            complement(1349..2125)
                     /gene="yaaA"
                     /locus_tag="AC16VR_05900"
     CDS             complement(1349..2125)
                     /gene="yaaA"
                     /locus_tag="AC16VR_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HIR6010725.1"
                     /translation="MLAVLSPAKTLDFETPSPTRKHSLPVFLDESKQLVETLRTQSPA
                     DLAQLMSMSTKLADQNYQRYTDWQTPFTAGNAKQAALAFKGDVYLGLEPGTFSERDFS
                     WAQKHIRILSGLHGILKPLDLIQPYRLEMGTRLKTRRGADLYAFWGQKITDELNAALA
                     EQKRPVLINLASNEYFDAVDPGRLNARIVTPTFKDLKNGRYRFMSFFAKRARGMMAGY
                     LVKNRVNTLKGLREFDWGGYYYSEDLSRGDDWVFLRDKPA"
     gene            2177..2896
                     /locus_tag="AC16VR_05905"
     CDS             2177..2896
                     /locus_tag="AC16VR_05905"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF025053.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR6010726.1"
                     /translation="MRIIGRTDRGRVRGSNQDAWACDAELGVAVLADGMGGLTGGEVA
                     SRAAVECVLGHLAGVPCIDADSVREAIAAANGRVLELSREQADGDMGTTLVVWARQAP
                     GRYLLGHVGDSRAYRLSGAVLEPLTRDHSVVQAMVDDGRLSEADALCAPDRNLITRAV
                     GLEVDVFADVVELEYEAEDAFLLCSDGLTDLLAPDELAEICDGHLGPAMRDLPDSLVN
                     AANAAGGYDNITVVLVYNCDP"
     gene            2916..3230
                     /locus_tag="AC16VR_05910"
     CDS             2916..3230
                     /locus_tag="AC16VR_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008171407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HIR6010727.1"
                     /translation="MSIQDRINERLQSRFDLHHLEVVNESGGHNVPPGSETHFKVILV
                     SPQFEGLRLIQRHRLVHETLAEELASGVHALAVHTYTDAEWRSRFGSAPMSPPCEGGG
                     RA"
     gene            3206..>3358
                     /locus_tag="AC16VR_05915"
     CDS             3206..>3358
                     /locus_tag="AC16VR_05915"
                     /inference="COORDINATES: protein motif:HMM:NF037630.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010728.1"
                     /translation="MRGRRAGVTSVVTFYKFVRLLDPSGLRDELDGMACELALKGTIL
                     LAGEGIN"
BASE COUNT          571 a         1022 c         1115 g          650 t
ORIGIN      
        1 gaagatggag ggaaacatgg aagccgtcgg cacctgattc cagtctgcca cggcggagcg
       61 aaagaccagc atgatggcca gaaagagggc aaagcctcgc caggtgaccc agtgctcgtg
      121 cagtcggccg gtcacgctcc tgatcagccc tgctgccggc ccgattcgaa gccggccagc
      181 tgctcgggcg tcgcctcggc ctggtagcgg gacttccaat ccgcgtaggg cataccgtag
      241 acccgctccc gcgccgcttc gtagtccatg gcgacgccgg cgtcctcgca ggcggcccga
      301 taccacttcg acaggcagtt gcggcagaaa ccggccaggt tcatcaggtc gatgttctgc
      361 acgtccttgt gctcgtcgag atgctccagc aggcgccgaa acaccgcggc ctctatggcg
      421 ttttcctgat ctttgtccac gacgaatccc aaagttgaca cgcgttggcc gcattctacc
      481 gaaaacttgc ggcctgggtg cttcggcaga aatcctgtgc ttcaatgccc gcaagtcaac
      541 cggctgacgg cagattgtgg agcttctcac caacccggag atttgggccg cgttcgtcac
      601 gctgacgatc ctcgagatcg tgctcgggat cgacaacatc atcttcatct ccatactcac
      661 cagtcgcctg ccgcctgagc agcgtgcccg cggccgcgtg ctcggtcttg gcctggccat
      721 gctcatgcgc attctgctgc tactctcgct tgccgggatc atgcggttga ccacggacct
      781 tttcgaggtg atgggccagg gcatatcggg ccgcgacctg atactgatct tcggcggcgc
      841 cttcctcatg gcgaaagcca cccgggaagt tcacaacgcc ctggaaggtg aagctcacga
      901 aggtcagggc gccggcgcgc cgacgttcgc gggcgttctg ttgcagatcg ccctgatcga
      961 catggtcttc tcgctggatt cggtgatcac ggcggtaggc ctggtggacg aggtgccggt
     1021 gatggttgcc gccgtgatct ccgcggtgct ggtgatgatg gtcgccgcgg gaccgatcag
     1081 tgatttcgtg gaagatcacc caacggtgaa gatgctcgcg ctggcgtttc tgcttctcat
     1141 cgggctcacg ctgatgggcg aaggcatcga gatccacacg cccaagggat acatctactt
     1201 tgccatggcg ttttcgttcg gagtggagat cctcaacatc cgggccaaga aacgcaggaa
     1261 ggatcacgtg atgctgcgca agacgcagct tgccgatctg gttgcctcgc ggtcggagaa
     1321 gggccggccg gattagccgg ccgccggctc aggcgggctt gtcacgcaga aagacccaat
     1381 cgtctccccg ggacagatcc tcggaatagt agtagccgcc ccagtcgaac tcgcgaagcc
     1441 ctttgagcgt gttgaccctg ttcttcacca ggtagccggc catcattccc cgcgcccgct
     1501 ttgcgaagaa ggacatgaag cggtacctgc cgttcttgag gtccttgaag gtgggcgtga
     1561 cgatccgcgc gttcaggcgg cctgggtcga cggcgtcgaa atactcgttg gaggcgaggt
     1621 tgatcagcac cgggcgtttc tgctcggcca gggcggcgtt cagctcgtcg gtgatcttct
     1681 gaccccagaa agcgtagaga tcggccccgc ggcgggtctt gagccgggtg cccatctcca
     1741 ggcggtacgg ttgtatgaga tcaagtggct tcaggattcc atgcaggccg gacagtatgc
     1801 ggatgtgctt ctgtgcccag ctgaagtcac gctcggagaa cgtgcccggt tccaggccga
     1861 ggtacacgtc gcccttgaac gccagggcgg cctgcttggc gttgcccgcc gtgaacggag
     1921 tttgccaatc ggtgtagcgc tggtagttct gatcggcgag ctttgtgctc atgctcatca
     1981 gctgtgccag gtctgccggg gactgagttc tgagcgtctc caccagctgc tttgactcgt
     2041 cgagaaaaac tggcaggctg tgcttgcgcg tgggggaggg cgtctcgaaa tctaatgttt
     2101 ttgccgggga tagaactgcc agcatttggc cggaggctcc tggattgatt cgggtgccaa
     2161 gtgtaacgga attcgcgtga gaatcatcgg acgcacggat cgcggccggg tgcgcggcag
     2221 caaccaggac gcctgggcgt gcgacgcaga gctcggcgtc gccgtgctcg ccgacggcat
     2281 gggggggttg accggcgggg aggtggcaag ccgggcggcg gtggagtgcg tcctgggaca
     2341 cctggccggc gttccctgca tcgatgccga cagcgtcagg gaggccatcg ccgcggcgaa
     2401 cggcagagtt ttggaacttt cccgcgagca ggctgatggc gacatgggaa cgaccttggt
     2461 ggtctgggcc cggcaggcgc ccggccgcta cctgctcggc cacgtgggcg actcgcgcgc
     2521 gtaccggctg agcggcgccg tcctcgagcc gcttacccgc gatcacagcg tcgtgcaggc
     2581 catggtggat gatggccggc tgagcgaagc ggacgccctg tgcgcgcccg atcgcaatct
     2641 gatcacccgg gcggtcgggc tcgaggtgga tgtgttcgcg gacgtggtgg agctcgagta
     2701 cgaagcggaa gatgcgtttc tgctctgctc cgatggcctc accgatctgc tcgctccgga
     2761 cgagctggcg gaaatctgcg acggccatct agggccggcc atgcgggatc ttccggacag
     2821 cctcgtgaat gccgcgaacg cggcgggggg atacgacaac atcaccgtgg tgctcgtcta
     2881 taattgtgat ccctgatggt ttgcaactgc tgtcgatgtc gatccaagac cggataaacg
     2941 agcgtcttca atcccgcttt gatctgcacc acctcgaggt ggtcaacgaa agcggcgggc
     3001 acaacgtgcc gcccgggtcg gagacccact tcaaggtgat tctcgtctcc ccgcagttcg
     3061 aaggcctgcg cctgatacag cgccaccggc tggtccacga gacgctcgcc gaggagttgg
     3121 cctccggcgt gcacgcgctg gccgttcaca cctacaccga cgccgagtgg cgctcgcgct
     3181 tcggcagcgc gcccatgtcg ccgccatgcg agggcggcgg gcgggcgtga ccagcgtcgt
     3241 cacgttctac aagttcgtgc gcctcctcga tccctcgggg ctgcgcgacg agctcgatgg
     3301 catggcttgc gagctggcgc tgaagggaac gatcctgctc gccggggaag gcatcaac
//
LOCUS       DBNHQR010000256         5590 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1353298, whole genome shotgun sequence.
ACCESSION   DBNHQR010000256 DBNHQR010000000
VERSION     DBNHQR010000256.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5590)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1353298"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..125
                     /locus_tag="AC16VR_05920"
     CDS             15..125
                     /locus_tag="AC16VR_05920"
                     /inference="COORDINATES: protein motif:HMM:NF023048.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HIR6010729.1"
                     /translation="MDCDDNNPDVHPDHNDTKGRWGRDGVDNDCNGTIDG"
     gene            complement(173..1417)
                     /locus_tag="AC16VR_05925"
     CDS             complement(173..1417)
                     /locus_tag="AC16VR_05925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HIR6010730.1"
                     /translation="MTSAARIAGLPSYFIVFVASMVAIGPFAIDTYLPAMPAMATNLG
                     VEIVSVNATLSTYLFGFAFGQMFGGPISDQIGRRRIGLLGLVVFSAASVLIALAESIE
                     VILWLRALQAVGGGFATVICMAMVRDAYEPMEAAKRFPVVMLVMLGAPLAAPAIGAAL
                     LSFGWPSIFIFLALYGLAVLVAFLPVPETASMRTGKLELGRILPQYAEVITRRVEGRL
                     VPARYILTQGLLMSGTFVFITNSSFIYLEYFGIAEEWFVVYFGVNILMMMVFTLITTR
                     LIHRVAPDRLWRAARGLQFLAVVMLALGSWLFDLNIWTLTGLLSVVIGANGMINPSAS
                     GLYLSYFDRLSGSAVSLMNVAVFLFGSVLGVVTGVFFDGTLKPIVFTMAVAITVGNLN
                     ALGIPPPKNLAPDASGESASAG"
     gene            complement(1436..3067)
                     /locus_tag="AC16VR_05930"
     CDS             complement(1436..3067)
                     /locus_tag="AC16VR_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007155086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6010731.1"
                     /translation="MGETVAEETVAADADAVVIGAGFAGLYMLHKLRQCGFSVRGFEA
                     GDDVGGTWYWNRYPGARCDVESLDYQFAFSDELQRGWTWSERYPTQAEILRYLGYVAD
                     TLDLRRDIRFETRVSAARFDEAHNLWRVTTDRGEELSARYVIAAVGCLSVAQIPDIPG
                     LQDFEGETYHTGNWPHQPVDFTGRTVGIIGTGSSGVQAIPVIAAQAKALVVFQRSPNF
                     SVPARNAPLPADRAERYKDEFLAAREAACRTDFGNTTWRSLPSALEADPDERTAEYER
                     RWQEGGAGFMVTYSDLMSDRAANDTAAEFLRGKIRAIVRDPETAELLSPRDHPIGAKR
                     ICVDTDYYETFNRENVKLVDVRSHPIERITASGISTTAGDYDLDCLIFATGFDAMTGP
                     LTSMDIRGRDGVSLRAAWQDGPRCYLGLGVAGFPNLFTITGPGSPSVLSNMVLSIEQH
                     VEWIADCLVKLRKDGAEAIEALPDAQDGWIEHVNEAADATLFPQGNSWYIGANVPGKP
                     RVFMPYTGGVRAYREKCEEVVGNSYEGFDIRGGAS"
     gene            complement(3080..4357)
                     /locus_tag="AC16VR_05935"
     CDS             complement(3080..4357)
                     /locus_tag="AC16VR_05935"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6010732.1"
                     /translation="MSEATESIETCPIHAIRPMLPENLAYPVAMNRRLRKDAPVYQDP
                     DSGIWFVSRYEDVINMSMDHKTFSSVMPRLSTGRADSAVDPEVAAAMAEGYPIPDTML
                     TQDPPLQRRYRKFVDGAFSPRSLKALEPFIEQTSNELIDAFIDQGHCEFLAEFGVQLP
                     LRVIASQIGCPMEDLPLLRKWTEAFIANLSQQLDQPGRVQAAKDMVECQHYFVERIEE
                     RRRAPTDDILSKLVNARFDDDKPLDNPECLSMLQQILVAGNETTSSSLTEGMWLLIRN
                     PDQYEMIRRDPSPEMISKLVEEVLRISSPSANMFRRTTRDVEVSGVTIPANSICFARF
                     AAANQDDEQFPDPLKFDITRPNLKEHVAFGKGVHHCLGAALSRREMNGGFKVIFERME
                     NFRLTPDTPEPVFMPNALLHGLARLDLSFDKIR"
     gene            4533..>5590
                     /locus_tag="AC16VR_05940"
     CDS             4533..>5590
                     /locus_tag="AC16VR_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010733.1"
                     /translation="MKPRLRLLLAACCVALLAGCDNDDDDDIVAPPAPEPVQTEPVNG
                     SGVKGPLAGAVVELYAIDLAQANIQGELIDDGSTGPDAAIQDLAVPADTTGKVLLLFV
                     VDDDTTDLTTGAAPLFDSLTTVVDAQRLLDGESVYASPLTTMAVDLAVAKGDSGSPYA
                     GNGDGTLDEAEFAAALDVAQGQVKSTLGFGMDSTIDIFTVPPLLTNDTDTADEQGDVA
                     AYRQAIEAVTAISAQIAADVEAAGGTASAQDAFNALTEDLTDGDLDGESEAGPIETVA
                     SLAEGELETIVTQDVTTLTIPGTETPIGEIESVLAAETADTGAETDTTDLESGAVSVD
                     PEPAETETDIDGDGVSDSA"
BASE COUNT         1019 a         1821 c         1868 g          882 t
ORIGIN      
        1 gctacacgtc ggacgtggac tgcgacgaca acaacccgga cgtgcacccc gaccacaacg
       61 acaccaaggg ccgctggggg cgggacggtg tcgacaacga ctgcaacggc accatcgacg
      121 gctgacgcag gtcacacgac tacgggcgcc cgcgggcgcc cgattttttc catcagcctg
      181 ccgacgccga ctctccgctt gcgtccggcg ccagattctt gggcggcggg atgccgagcg
      241 cgttcagatt gcccacggtg atggccaccg ccatggtgaa gacgatgggc ttcaacgtgc
      301 cgtcgaagaa cactccggtg accacgccga gcacgctgcc gaagaggaag acggccacgt
      361 tcatcagcga caccgcgctg ccgctcagcc gatcgaagta ggacaggtag aggccggacg
      421 cggacgggtt gatcatcccg ttggcgccga tgacgacgga cagcaagccg gtcagcgtcc
      481 agatgttcag atcgaacaac cacgagccca gggcaagcat caccacggcc agaaactgca
      541 gcccgcgcgc cgcgcgccag agacggtctg gcgcaacccg atggatcagc cgggtagtga
      601 tcaatgtgaa caccatcatc atcaggatgt tcacgccgaa atagacgacg aaccactcct
      661 cggcaatgcc gaagtattcc aggtagatga acgaggagtt ggtgatgaac acgaacgtcc
      721 cggacatgag aaggccctgg gtgaggatgt agcgcgccgg cacaagcctg ccctcgaccc
      781 gccgggtgat cacctcggcg tactggggca aaatccggcc gagctcgagc ttgcccgttc
      841 gcatcgaagc ggtttccggc accggcagga aggcgacgag cacggccagg ccgtacaggg
      901 cgaggaagat gaagatgctc ggccagccga agctcagcag cgcagcgccg atcgccggcg
      961 ccgccagggg ggcgccgagc atgacaagca tcaccacggg aaagcgcttg gccgcttcca
     1021 tgggctcgta ggcatcacgc accatggcca tgcagatcac cgtggcgaag ccgccgccaa
     1081 cggcctgcaa cgcccgcagc cagaggatga cttctatgga ttcggccaag gcgatgagca
     1141 cggacgcggc agagaagacg actaggccca gcagcccgat gcggcgccgg ccgatctgat
     1201 cggagatcgg gccgccgaac atctgcccga aagcaaagcc gaagaggtac gtgctgagcg
     1261 tggcgttgac cgagacgatc tccacgccga ggttcgtcgc catcgccggc atggcgggca
     1321 ggtaggtatc gatggcaaag ggcccgatcg ccaccatgga cgcgacgaac acgatgaagt
     1381 agctggggag acccgcgatc cgggcagcgc tcgtcacgtc agttgccggc cgggctcagc
     1441 tcgcgccgcc gcgaatgtcg aagccttcgt aactgttgcc gaccacctct tcgcacttct
     1501 cgcgatacgc gcgcacgccg ccggtataag gcatgaagac cctgggtttg cccgggacgt
     1561 tggcgccgat gtaccaggag ttgccctgag ggaacagcgt ggcgtcggcg gcctcgttga
     1621 cgtgctcgat ccagccgtcc tgggcgtcgg ggagagcctc gatggcctcc gcgccgtcct
     1681 tacgcagctt caccaggcag tcggcaatcc actccacgtg ctgctcgatg gatagcacca
     1741 tgttgctcag cacggaaggg ctgcccggcc cggtgatggt gaagaggttg gggaatcccg
     1801 caacgcccag gcccagatag cagcgcggcc cgtcctgcca ggccgccctc aggctgacgc
     1861 catcgcgacc gcggatgtcc atggacgtga gcgggcccgt catggcatcg aaaccggtag
     1921 cgaagatcag gcagtcgaga tcgtagtcgc cggccgtcgt gctgatcccg ctcgccgtga
     1981 tgcgctcgat gggatgagaa cgtacgtcca cgagcttcac gttctcgcgg ttgaaggttt
     2041 cgtagtagtc ggtgtccacg cagatgcgct tggccccgat ggggtggtcg cggggcgaga
     2101 gcagctcggc cgtctcaggg tcgcggacga tggcgcggat ctttcctctg agaaattcgg
     2161 ccgcggtatc gttggccgcc cggtcggaca tgaggtcgct gtaggtgacc atgaagccgg
     2221 caccgccttc ctgccagcgg cgctcgtact ccgccgtgcg ctcgtccgga tcggcttcca
     2281 gggccgacgg caggcttctc cacgtggtat tgccgaagtc ggtccggcag gccgcttcgc
     2341 gcgcggccag aaactcgtcc ttgtagcgct cggcccgatc ggccggcagg ggagcgttgc
     2401 gggccggcac gctgaagttg ggcgagcgct gaaataccac gagcgccttt gcctgggcgg
     2461 cgatcacggg tatggcctgc acgcccgagg agcccgtgcc gatgatgccg acggtgcgcc
     2521 cggtgaaatc caccggctgg tggggccagt tacccgtgtg ataggtctcg ccctcgaagt
     2581 cctgcagccc ggggatgtcg gggatctggg ccacggagag gcagcccacg gcggcgatca
     2641 cgtagcgggc gctgagctcc tcgccccggt ccgtggtcac ccgccaaagg ttgtgcgcct
     2701 cgtcgaagcg cgcggcgctc acccgggtct cgaaccggat atcccgacgc agatcgaggg
     2761 tgtccgccac gtagccgagg tagcgcagga tctcggcctg ggtggggtag cgctcggacc
     2821 acgtccagcc gcgctgcagc tcgtcggaga aggcgaactg gtagtccagg ctctccacgt
     2881 cgcagcgcgc gcccgggtag cggttccagt accaggtgcc gcccacgtcg tcgcctgcct
     2941 cgaagccgcg taccgagaag ccgcactgcc gcagcttgtg cagcatgtag aggccggcga
     3001 agcccgcacc gatgacgacg gcatcggcgt cagcagcgac ggtctcctct gcaacggtct
     3061 cccccacgcc cgcttgcgat caacggatct tgtcgaagga aagatccagc cgcgccagcc
     3121 cgtgcagcaa ggcgttcggc atgaacaccg gctccggcgt gtccggcgtc aggcggaagt
     3181 tctccatgcg ctcgaagatg accttgaacc caccgttcat ctcacggcgc gacagcgccg
     3241 cgccgagaca gtggtgcacg cccttgccga acgctacgtg ctccttcagg ttgggccggg
     3301 tgatgtcgaa cttcagcgga tccggaaact gctcgtcgtc ctggttggcc gccgcaaagc
     3361 gggcgaagca gatggagttg gcggggatgg tcacgccgct cacttccacg tcccgcgtgg
     3421 tgcggcggaa catgttggcc gacgggctgg agatgcgcag cacctcctcc accagcttgc
     3481 tgatcatctc cggcgacggg tcccggcgga tcatctcgta ctggtcggga ttgcggatca
     3541 gcagccacat gccttcggtg agggacgacg aggtggtctc gttgccggcc accagaatct
     3601 gctgcagcat cgacaggcat tccgggttat ccagcggctt gtcgtcatcg aagcgggcgt
     3661 tgacgagctt ggagaggatg tcgtccgtcg gcgcgcggcg gcgctcctcg atgcgctcca
     3721 cgaagtagtg ctggcactcc accatgtcct tcgccgcctg cacccggccc ggctggtcga
     3781 gctgctggga gagattcgca atgaaggctt ccgtccactt cctgagcagc ggcaggtcct
     3841 ccatggggca gccgatctgg gaggcgatga cccgcagcgg cagctgcacg ccgaactcag
     3901 cgagaaactc gcagtggccc tggtcgatga aggcatcgat gagctcgttg gatgtctgct
     3961 cgatgaaggg ctccagggcc ttgaggctcc ggggcgagaa cgcgccgtcc acgaacttgc
     4021 ggtagcggcg ctgcaggggc ggatcctggg tcagcatggt gtccgggatc gggtagcctt
     4081 cggccatcgc cgccgccacc tccgggtcga cggcgctgtc cgcccgaccg gtcgacaggc
     4141 gcggcatgac gctggagaac gtcttgtgat ccatggacat gttgatcacg tcctcgtatc
     4201 gggatacgaa ccagatgccg ctgtccgggt cctgatagac gggtgcgtcc ttgcgcagcc
     4261 gccggttcat ggccaccggg taggccaggt tctccggcag catgggccgg atggcgtgga
     4321 tcgggcaggt ttcgatggat tctgttgctt cgctcatggg tctttgtctc tgaatggcag
     4381 gccgatgcgc ccctcagcgc tgcgtcgggg agcggggaaa ctagcgcacc ttgcctttcg
     4441 ttgccagcgt attgcccgca ggatctaaat gaggtaaaaa aagtttctga tcaacaagat
     4501 tgactaataa ctggcaaata gggggagatc gcttgaagcc aagactccga ttactgctgg
     4561 cggcctgttg cgttgcgctg ctcgccggat gtgacaacga cgacgatgac gatatcgtcg
     4621 cgcccccagc accagagccc gttcaaaccg aacccgtcaa cggttccggc gtgaaggggc
     4681 ctctggccgg cgcggtggtg gagctctacg ccatcgacct tgcccaggcg aacatccagg
     4741 gcgagctgat cgacgacggc tccacgggtc ccgacgccgc catccaggac ctggcggtgc
     4801 ctgcggacac cacgggcaaa gtcctgctcc tattcgtcgt cgacgacgac accacggacc
     4861 tcaccactgg cgccgcgccg ctcttcgact ccctcaccac ggtggtcgat gcccaacggc
     4921 tcctcgacgg cgagagcgtc tacgcctccc cgctgaccac catggccgtg gatctggccg
     4981 tggccaaagg cgactcgggc tcgccctacg ccggcaacgg cgacggcacg ctggacgaag
     5041 cggagttcgc cgccgccctg gatgtggccc agggccaggt gaagtccacc ctcggcttcg
     5101 gcatggatag caccatcgac atcttcacgg tgccgccgct actgaccaac gacacggaca
     5161 ccgccgatga gcagggggac gttgccgcgt atcgccaggc catcgaggcg gtgaccgcca
     5221 tctccgccca gatcgccgca gacgtggaag ctgccggcgg cacggccagc gcccaggacg
     5281 ccttcaacgc gctcacggaa gacctcacgg acggcgacct tgacggcgag agcgaggccg
     5341 gacccatcga gacggtcgct tccctcgccg agggcgagct cgagaccatc gtcactcagg
     5401 acgtgaccac cctgacgatt cccggcacgg agacgcccat cggcgagatc gagagcgtgc
     5461 tggccgccga aaccgcggac acgggcgcgg agaccgacac cacggacctg gagagcgggg
     5521 ccgtctcggt ggatccggag cccgccgaga ccgagaccga catcgacggt gacggcgtga
     5581 gcgacagcgc
//
LOCUS       DBNHQR010000257         5948 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_404227, whole genome shotgun sequence.
ACCESSION   DBNHQR010000257 DBNHQR010000000
VERSION     DBNHQR010000257.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5948)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5948)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5948
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_404227"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..2470)
                     /locus_tag="AC16VR_05945"
     CDS             complement(95..2470)
                     /locus_tag="AC16VR_05945"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HIR6010734.1"
                     /translation="MLSDLRVLDLSTDLGLMCGQLLADMGAEVQQWVLPEDAARLDTC
                     YWQAYTLGKGVQLIDWQADPEAVRRQVGCVDVIIESAPPGLMARLGLGYEELARINPE
                     VVHVSITGFGSTGPKADYEFSDLISAAAAGLVYVTGSEGQAPVRTTCPQAHGHASSDA
                     AVGVLIALFERARTGRGQHVDVSAQQSTTLALLNRALDGPVGQAKAERSAYGMTINGV
                     HVQNQFQARDGWVVCLPGVLPPIAAFMERLMAWVFEEGLCREEDLRWDWGGVALQIMQ
                     GQVTPEQWAPVTAGMEALIAGRTKAELMSEAISRRLLIAPVFSVADLLDSDHLAQRGF
                     LVEGGAGLRLGPFARLSESPLPLTESGPRFWQERSAFDARPLAASPSGRDESGGDTEG
                     GGGPLAGLKVLDLFWVVAGPGATRMLADYGATVVHVESTRRQDMVRNVPPYIDGVPDP
                     ERAACHHSTNTNKLNLTLDISREEGRAVLADLIRWADVFTESFAPGAVARMGFDYATV
                     RTLNPNIIMISSCLMGQTGEWSGYAGFGNNGSAVSGFHALTGHPGQPPTGVFGPYTDF
                     TSVRFNALAILGAVMHGRRTGNGQYIDMSQGEAALHFLAPECLRYLRDGRVRGAEGNR
                     DEHFAPQGVYPTAGFDRWLALSVRSDAEWRALCALAGFDDLSNMSVEERAAAHDELDR
                     RLETWMETRPGEELEAELQSAGIAAHRTLDTHELGDDPQLVHRGHFIPVAHREFSNAV
                     VESSRLTLSASRAARPGTAPWFGIDNERVLSEILGYDGDRVRELASADVLS"
     gene            complement(2460..3326)
                     /locus_tag="AC16VR_05950"
     CDS             complement(2460..3326)
                     /locus_tag="AC16VR_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010735.1"
                     /translation="MVAFRRCLVIALWCLSAHALGYDGRIHQDLTFLAAKHFNRCTAG
                     TEVPPLTPLQIRYLAKTNVGLADRNAFARMFNWRYYDRAAEAEHSVMWAIDTRFHEHF
                     NEILARIDKADSMGKLYSEVGRIINYLQLVSSPPHTVPVFTSRFWRFSFSDRFDGFPV
                     DVEAVGQAIGEDCSFLAAEEEYAGVLRSIAADTIAAVRAPIEGMPATWEAFWEIADDP
                     EKFGDYGPAGNSFGRKTEFGCAQQRCVLLRADPLYAAFAAQRHAAAVKATLAAMLIVQ
                     RSQIATEVAGAQ"
     gene            complement(3313..3816)
                     /gene="folK"
                     /locus_tag="AC16VR_05955"
     CDS             complement(3313..3816)
                     /gene="folK"
                     /locus_tag="AC16VR_05955"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HIR6010736.1"
                     /translation="MVLVGLGSNRGDSVGIVRAAMGRLRGFADGDTRCSDLWRTSPVD
                     CPPGSEDFINAAVSFRAAPGLTPESLLADLKALEAEYGRTGQRLRNAPRELDLDLLLF
                     DAETRDTADFVLPHPRAADRLFVLAPAAQVAPDVVWPEIGLTIRELLDELDSDERVVR
                     LEAHGCL"
     gene            3977..5059
                     /locus_tag="AC16VR_05960"
     CDS             3977..5059
                     /locus_tag="AC16VR_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007974217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HIR6010737.1"
                     /translation="MKAAVLREINKPLEIEDIQHGDPGPREVLVRTVAAGVCHSDLHF
                     QNGSYPYPLPAVLGHESAGIVEAVGSDVNYVKPGDHVITCLSAFCGHCEYCLTGHMSL
                     CQEPELQRPMDQPQRLNKGDENIWQFLNLSSFAEYMLVHEHAIAKIREDMPLDRAALI
                     GCGVTTGVGAVIHTAKVEPGSTVAVIGCGGIGLSAINGAAIAGAARIIAIDTVESKLD
                     LARKFGATDVVNARESDVIEAVREMTGGGVHYSFEAIGLKQTAEQSFKILQRGGTATV
                     IGMIPVGTNIELHGPEFLMERKMQGSNMGSNRFRVDMPRFVEFYLQGRLHLDDMISRR
                     IRLEDVNDGLAALETGELARSVIMFD"
     gene            complement(5080..5424)
                     /locus_tag="AC16VR_05965"
     CDS             complement(5080..5424)
                     /locus_tag="AC16VR_05965"
                     /inference="COORDINATES: protein motif:HMM:NF012803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HIR6010738.1"
                     /translation="MLRDGQPQQVTLKIGEDELEKVRGRRIDRRLAGTQLQNFRNQDE
                     PGMGAGVLVLDIDPDSDAYRFGLRPGDILVAANRRAIVNLAELREVARLDDGQLLLRV
                     YRSGQFGYVAIR"
     gene            complement(5437..>5948)
                     /locus_tag="AC16VR_05970"
     CDS             complement(5437..>5948)
                     /locus_tag="AC16VR_05970"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HIR6010739.1"
                     /translation="IGNPFGLRQTVTSGIISALGRSGLGIEGYEDFIQTDASINPGNS
                     GGALVDLAGRLVGINTAILAPSGGNVGIGFAIPSNMVRAIMDQLIEHGEVRRGYLGLA
                     VQGLNVDLAEAFGVDRREGVVVVEVEPDSAAESGGLQAGDIITRVGDREIGKIADFHS
                     QAAVTFVGD"
BASE COUNT         1036 a         2031 c         1935 g          946 t
ORIGIN      
        1 tgccgggcct gctcggtcag cccgctggcg gtgcgatcag tgagattcca gggcgttatg
       61 ccaagccgca tctgcatgcc acctcaccac gccgtcacga cagcacgtcc gcagatgcca
      121 gctcgcggac ccggtcgccg tcgtagccga gaatctcgct tagcacgcgc tcgttgtcga
      181 tgccgaacca cggcgccgtg ccgggtcggg cggcgcggct tgccgacagg gtcagccgtg
      241 agctttccac cacggcgttc gagaactccc ggtgcgcaac gggaatgaag tgaccccggt
      301 gaaccagctg gggatcgtcg ccgagctcgt gggtatccag ggtccggtgg gcggcgatgc
      361 cggcagattg cagctccgct tccagctcct ccccggggcg ggtctccatc cacgtttcca
      421 ggcgccggtc gagctcgtcg tgagcggccg cccgttcctc gaccgacatg ttcgagaggt
      481 cgtcgaatcc cgccagggca cacaacgcgc gccactcggc gtcgctgcgc accgaaagcg
      541 ccagccagcg atcgaacccg gcggtcgggt agacgccctg gggggcgaag tgctcatcgc
      601 ggttgccttc ggcacccctg acccgaccat cgcgcaggta acgcaggcac tcgggcgcga
      661 gaaagtgcag cgccgcctcc ccctgggaca tgtcgatgta ctggccgttg ccggtccgac
      721 ggccgtgcat cacggcgccg agaatggcca gggcgttgaa gcgcactgag gtgaaatcgg
      781 tataggggcc gaagacgccc gtgggcggct ggcccggatg gccggtgagc gcgtgaaagc
      841 ccgacactgc cgagccgttg ttgccgaacc ccgcgtagcc gctccactcc cccgtctggc
      901 ccatcaggca gctcgagatc atgatgatgt tcgggttgag cgtcctcacc gtggcgtagt
      961 cgaagcccat ccgggccacc gcgccggggg cgaaggattc ggtgaagacg tcggcccagc
     1021 ggatcaggtc ggcaagaacc gcacgaccct cttcccgact gatgtccagc gtcaggttga
     1081 gcttgttggt gttggtgctg tgatggcagg cggcgcgctc cgggtccggg accccgtcga
     1141 tgtagggcgg cacgttcctg accatgtcct gccgccgagt ggactccacg tgcaccacgg
     1201 tggccccgta gtcggcgagc atgcgcgtgg cgccggggcc ggccaccacc cagaaaaggt
     1261 cgaggacctt caatccggcc agcggcccgc cgcccccttc ggtgtcccca cccgactcat
     1321 cccggccgga cggggaggca gccaggggtc tggcgtcgaa agcagacctt tcctgccaga
     1381 atcgcggccc gctttcggtc agcggcagcg gcgactctga caaacgggca aacggcccga
     1441 gccgaaggcc cgctccgccc tccaccagga agccgcgctg agcgaggtga tcgctgtcca
     1501 gaaggtccgc tacagagaac accggagcga tgagcagccg ccggctgatg gcctcggaca
     1561 tcagctctgc cttggtgcgc ccagcgatca gcgcttccat gccggcagtc accggggccc
     1621 attgctccgg ggtaacttgc ccctgcatga tctgaagggc cacgccgccc cagtcccacc
     1681 tgaggtcttc ctcgcggcaa aggccttctt cgaacaccca cgccatcagc cgctccatga
     1741 aagcggcaat gggtggaagc acacccggca ggcataccac ccagccgtca cgggcctgaa
     1801 actggttctg aacgtgcacg ccgttgatcg tcatgccgta agcgctgcgc tccgccttgg
     1861 cctggccaac gggaccgtca agcgcccgat tgagaagcgc cagcgtggtc gactgctggg
     1921 cagacacatc gacgtgctga ccgcggcccg ttcgcgctcg ctcgaagagc gcaatgagca
     1981 cgcccaccgc cgcatcgctc gaggcgtgcc cgtgggcctg cggacacgtc gtgcgaacgg
     2041 gcgcctgccc ttcgctgccc gtcacgtaga ccagccccgc tgcagccgcg gaaatcagat
     2101 ccgagaactc gtaatccgcc ttcgggccgg tgcttccgaa cccggtgatg gagacatgaa
     2161 cgacctccgg gttgatgcgc gcgagctcct cgtagccgag gcccaggcgc gccatgagcc
     2221 cgggcggggc ggactcgatg atcacgtcga cgcagccgac ctgccggcga acggcctcgg
     2281 gatcggcctg ccagtcgatc agctgaacgc ctttgccgag cgtgtaggcc tgccagtagc
     2341 aggtgtcgag tcgggccgca tcttcgggca ggacccattg ctgaacttcc gcccccatgt
     2401 cggcgagcag ctgcccgcac atcaacccga gatccgtgga gagatcgagc acgcgaagat
     2461 cactgagcac cggcaacctc cgttgcaatc tggctgcgct gcacgatgag catggctgcc
     2521 agggtagctt tcaccgccgc cgcgtgtcgt tgggcggcaa acgcggcgta gagcgggtcg
     2581 gcgcgcagga gcacgcagcg ctgctgcgcg cagccgaact cggtcttgcg gccaaagctg
     2641 ttgccggccg gaccgtagtc accaaacttc tccggatcgt cggcgatttc ccagaaggcc
     2701 tcccaggtgg ccggcatgcc ttcgatgggc gcgcgtaccg ccgcgatggt atccgcggcg
     2761 atggagcgga gaacccccgc gtattcctcc tcggcggcga ggaaggaaca gtcctcgccg
     2821 atggcctgcc ccacggcttc cacgtccacc gggaatccat cgaagcggtc gctgaagctg
     2881 aaccgccaga accgcgaggt gaataccggc acggtgtgag gcggggacga caccagctgc
     2941 aggtagttga tgatccggcc gacctcgctg tacagcttcc ccatgctgtc ggccttgtcg
     3001 atgcgcgcaa gaatctcgtt gaaatgctcg tgaaagcgcg tgtcgatcgc ccacatcacc
     3061 gagtgctccg cctcggcggc ccggtcgtag tagcgccagt tgaacatgcg ggcgaacgcg
     3121 ttccgatccg cgaggcccac gttcgtcttg gcgaggtagc ggatctgcag cggcgtcagc
     3181 ggcgggacct cggtgcccgc ggtgcagcgg ttgaagtgct ttgccgccag aaaggtcagg
     3241 tcctgatgaa tccggccatc gtacccgagc gcatgggccg acaggcacca gagcgcgatt
     3301 accaggcaac gcctaaaggc aaccatgcgc ttccagacgc accacgcgct cgtcgctgtc
     3361 gagctcgtcg agcagctccc ggatcgtcaa cccgatttcc ggccagacca cgtccggtgc
     3421 cacctgcgcc gccggcgcga gcacgaagag gcgatcggcg gcgcgcgggt gcggcaggac
     3481 gaagtcggcc gtgtcccgcg tctcggcgtc gaacagcagc aggtcgagat ccagctcccg
     3541 gggggcgtta cgcaagcgct gcccggtccg gccatattcg gcctcgagcg ctttgagatc
     3601 agcaagcagg ctctccggcg tcaacccggg ggcggcacga aaggagaccg ccgcgttgat
     3661 gaagtcttcc gaccccggtg ggcaatccac cggcgacgtg cgccacagat cggagcagcg
     3721 cgtgtcgccg tcggcaaacc cacgaagcct gcccatggcg gcgcggacga taccgacgct
     3781 gtcgccccga ttggatccaa gaccaaccaa taccatcgtt tttccggtgt cctagcaggc
     3841 atcccccagg gccaatacta acctctgcta tcccccggta gtaagggaga tcgggcgccg
     3901 ggaccggcgg cagatggtag taatcggacc atgcgtttgg cacactcaaa cgtcggacac
     3961 gtacagggag aggcccatga aagccgccgt tttgcgcgag atcaacaagc cactggaaat
     4021 cgaagatatc cagcacggcg acccgggccc acgcgaggtg ctggtgcgca cggtggcggc
     4081 cggcgtgtgc cattcggatc tgcacttcca gaacggctcg tatccatatc ccctgccggc
     4141 cgtgctcggc catgagtccg ccggcatcgt cgaggccgtg ggcagcgacg tcaactacgt
     4201 caagcccggg gatcatgtga taacgtgtct ttcggcgttc tgcggtcact gcgagtactg
     4261 cctgaccggc cacatgtcgc tgtgccagga gcccgagctg cagcggccca tggaccagcc
     4321 gcagcgcctc aacaagggcg acgagaacat ctggcagttc ctgaacctgt cctcgttcgc
     4381 cgagtacatg ctcgtccacg agcacgccat tgcgaaaatc cgggaggaca tgcctctgga
     4441 tcgggccgcc ctcatcggct gtggcgtgac gacgggcgtc ggggccgtca ttcacaccgc
     4501 caaggtggag ccgggcagca cggtcgccgt gatcggctgt ggcggcatcg ggctctccgc
     4561 catcaacggg gccgccatcg ccggcgccgc ccggatcatt gccatcgaca ccgtggaaag
     4621 caagctcgat ctggcgcgca aattcggcgc caccgacgtc gtgaatgcca gggagagcga
     4681 cgtcatcgag gcggtgagag agatgaccgg tggcggcgtg cactactcct tcgaggccat
     4741 tggtctcaag cagacggccg agcagtcttt caagatcctg cagcgcggcg gcaccgcgac
     4801 cgtcatcggc atgattcccg tgggcacgaa catcgagctg catggacccg agttcctcat
     4861 ggagcgcaag atgcaggggt cgaacatggg gtccaaccga ttccgcgtgg acatgccgcg
     4921 attcgtggag ttctacctgc agggccgcct gcatctggac gatatgatct cccgccggat
     4981 ccgcctggaa gacgtcaacg acggcctggc cgctctggag accggagagc tggcgcgcag
     5041 cgtcatcatg ttcgactaaa cgcccggccg ggccgccggt cagcgaatgg cgacgtaccc
     5101 gaactggcct gagcggtaca cccggagcag cagctgaccg tcgtcgagcc tggcgacttc
     5161 cctcagctct gcgaggttga cgatggcccg acggttggcg gccacgagaa tgtctccagg
     5221 tcgcagcccg aaccggtaag cgtcgctgtc cgggtcgatg tcgagcacca gcacgccggc
     5281 acccatgccc ggttcgtcct ggttgcggaa gttctgcaac tgcgttcccg ccaaccggcg
     5341 atcgatgcgg cggccgcgca ccttctccag ctcgtcctcg ccgatcttga gggtgacctg
     5401 ctgcggctgg ccgtcgcgaa gcacctcgat ccggacctag tccccgacga acgtcaccgc
     5461 cgcctggctg tggaaatcgg cgatcttgcc gatctcccga tcgcccacgc gagtgatgat
     5521 gtcaccggcc tgaagcccgc cggactccgc ggcgctgtcc ggctcgacct caaccacgac
     5581 cacaccttcg cggcggtcga cgccgaacgc ttccgcgaga tccacgttca gcccctgaac
     5641 ggctagaccg aggtagccgc ggcgcacctc gccgtgctcg atcagctgat ccatgatcgc
     5701 ccggaccatg ttcgagggaa tggcgaagcc gatgccgacg tttccgccgc tcggcgccag
     5761 gatggcggtg tttatcccca ccagacgccc ggccaggtcc acgagggcgc cgccggaatt
     5821 gcccgggttg atggacgcat cggtctggat gaagtcctcg tacccctcga tgcccaggcc
     5881 gctgcgaccc agcgcgctga tgatcccgct cgtcaccgtc tgccgcagcc cgaagggatt
     5941 gccgatgg
//
LOCUS       DBNHQR010000258         2471 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_658372, whole genome shotgun sequence.
ACCESSION   DBNHQR010000258 DBNHQR010000000
VERSION     DBNHQR010000258.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_658372"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="AC16VR_05975"
     CDS             complement(<1..845)
                     /locus_tag="AC16VR_05975"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6010740.1"
                     /translation="MNGPIAWFARNHIAANLLLAVMVLAGLLSLPIMPQKDFPDLDYK
                     LITVSVRYLGAAPEEVEDGVCIRIEEAIEGLQGIERIRSNAVEGRCTVNVELFENADD
                     NKVLGDVKNRVDAIETFPQEAEKPIITLVAAERTVVEIAVTGPSDERELKSIAQQVRD
                     DLAALPGITRVDVSGVRPYEISIEISEATLRRYGLSFDEVANAVRAASLDLPGGAIKT
                     ESGEVLLRTEGQAYRGSEFEDLVILTRGDGTRVFLRDLATVVDGFEDTDERVWFDNKP
                     AALVQV"
     gene            complement(842..2005)
                     /locus_tag="AC16VR_05980"
     CDS             complement(842..2005)
                     /locus_tag="AC16VR_05980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6010741.1"
                     /translation="MNRRLQAALVLLGGFAVAVLVFLSGPKMQPQAPSAIPPLVRVIE
                     AEPREVQMTSHTHGTVVPRTESDLVPEVDGRVTAVSPSLVSGGFFNQGDVLLEIEPVD
                     YEVALEHARAGLARAESDLENARKSLTRIQDLVRRGATSDSDRDDAANRERIAQATLR
                     EARARLSRAERDLDRTRLVAPYQGRVRNERVDVGQFVKRGNPVATIYAVDFAEVRLPI
                     QDEELAFLDLPLGYTGDESSIPIPVTLRARFAGVEHEWQGEVVRTEGELDPATRMVHV
                     VARVPEPYVSRDGRPPLSVGLFVDADIQGKTLSDVVILPRSALRGGDRVLVVDQDERL
                     RFRPVDVYRVAEEEVYIVGGLARGERVCISPLQSTSDGMLVRVADVEQRAAPS"
     gene            complement(2002..>2471)
                     /locus_tag="AC16VR_05985"
     CDS             complement(2002..>2471)
                     /locus_tag="AC16VR_05985"
                     /inference="COORDINATES: protein motif:HMM:NF037008.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010742.1"
                     /translation="IAPINDERMRRLRQLQASSSSPSVEALVRAFVDLEFALTDNEHL
                     DQFLAHLFAEPKSVSVPLLEKTFAPVVQAYFAALHAALPDVPVEVLRWRFHLMIGSMI
                     QLARFRHPLNFFQADSPPAEGSLQAGIDQLVEFVVAGLTQPGTSGDGASGDGE"
BASE COUNT          424 a          825 c          791 g          431 t
ORIGIN      
        1 acctgcacga gcgcggcggg cttattgtcg aaccagaccc gctcgtcggt gtcctcgaag
       61 ccgtcgacca ctgtggccag gtcccgcagg aacacacggg taccatcccc gcgcgtgagt
      121 atcaccaggt cctcgaactc tgagccccgg tatgcctgac cctcggtgcg cagcaggact
      181 tcgccgcttt cggtcttgat ggcgccgcct ggaaggtcca gagaggcggc gcgcacggcg
      241 ttggcgacct cgtcgaagct caaaccgtag cggcgcaggg tcgcctcgga aatttcgatg
      301 gagatctcgt agggccgcac gccagagacg tccacccggg tgattcccgg aagagctgcc
      361 agatcgtcgc gcacctgttg agcaatggac ttgagctccc gctcgtcgct cgggccggtc
      421 acggcaatct cgaccacggt tctctctgcc gcgaccagcg tgatgatggg tttctcagcc
      481 tcctggggga acgtctcgat ggcgtcgacc cgattcttga cgtcgccgag aaccttgttg
      541 tcatcggcgt tctcgaacag ctccacgttc accgtgcagc ggccctccac ggcattggat
      601 cggatgcgct cgatcccctg taggccctcg atggcttcct cgatgcggat gcacacgccg
      661 tcctcgacct cttcgggagc ggcacccagg tagcgcaccg acaccgtgat cagtttgtag
      721 tccaggtccg ggaagtcctt ttgaggcatg atcggcaggg atagcaggcc ggccagaacc
      781 atgaccgcaa gcagaaggtt ggctgcgatg tggttgcggg caaaccaggc gatggggccg
      841 ttcatgaggg cgcggctcgc tgctcgacat cggcaacgcg aaccagcatg ccatcgctgg
      901 tgctctgcag cggcgagatg cagacccgct cgccccgggc caggccgccg acgatgtaga
      961 cctcctcctc tgccactcgg tagacgtcaa cgggcctgaa gcgcagcctc tcgtcctgat
     1021 ccacgacgag cacccgatcg cctcccctca gtgccgatcg gggcagaatg accacgtcac
     1081 tgagcgtttt gccttgaatg tccgcatcga cgaagagccc gaccgacaga ggcggccgcc
     1141 cgtcccggct gacgtagggc tcgggaactc ttgcgactac gtgcaccatg cgggtcgccg
     1201 ggtccagctc accttccgta cgcacgacct cgccctgcca ctcatgctcc acgccggcga
     1261 aacgggcacg cagggtcacc ggtatgggga tcgaagactc gtcgcccgta tacccgagcg
     1321 gcagatcgag aaacgcgagc tcctcgtctt gaatgggtag ccggacctcc gcgaagtcca
     1381 cggcgtagat cgtcgccacc gggttgcccc gcttgacgaa ctggccgacg tccacccttt
     1441 cgttgcgaac ccgcccctgg tagggcgcga ccaggcgggt cctgtccagg tcgcgttccg
     1501 ctctggagag ccgcgcccgc gcttcgcgca gcgtggcctg agcaatgcgt tcccggtttg
     1561 ccgcgtcgtc ccgatccgag tcgctggtcg ctccgcggcg gacgagatcc tgaatgcggg
     1621 tcagtgactt cctggcgttc tccagatcac tctctgccct ggccaaacct gcccgggcat
     1681 gctcgagggc cacctcgtag tcgacgggtt cgatctccag cagcacgtcg ccctggttga
     1741 agaaaccgcc ggagacgagg gacggcgaga ctgccgtgac acggccgtcc acctccggca
     1801 ccagatcgct ttccgtgcgc gggaccaccg tgccgtgggt gtggctcgtc atctgcacct
     1861 cacggggctc ggcctcgatg acccgtacga gcggaggaat ggcgcttggc gcctggggct
     1921 gcattttcgg cccgctcaga aacaccagca cggccacggc aaagccccct aggagcacca
     1981 gcgcggcctg aagccttcga ttcattcacc gtctccgctg gcaccgtctc cgctggttcc
     2041 cggctgggtg agccccgcaa cgacgaactc cacgagctga tcgatgcctg cttgcaggga
     2101 gccttctgcg gggggcgagt ctgcctgaaa gaagttcaat gggtgtctga accgcgccag
     2161 ctggatcatc gatccgatca tgaggtgaaa gcgccagcgc aatacctcca cggggacgtc
     2221 gggcagcgcg gcgtgcaggg cggcgaagta ggcctgcaca accggcgcga aggtcttctc
     2281 gagcagcggc acggagacgg atttcggttc cgcgaacaga tgggcgagga actggtcgag
     2341 gtgctcgttg tccgtgagcg cgaattccag atcgacgaag gcgcgaacga gcgcttccac
     2401 cgaaggcgag ctggacgacg cctgaagctg gcgcaggcgc cgcatgcgct cgtcgttgat
     2461 tggcgcgatg c
//
LOCUS       DBNHQR010000259         2043 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_531369, whole genome shotgun sequence.
ACCESSION   DBNHQR010000259 DBNHQR010000000
VERSION     DBNHQR010000259.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_531369"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..1428
                     /gene="gspL"
                     /locus_tag="AC16VR_05990"
     CDS             184..1428
                     /gene="gspL"
                     /locus_tag="AC16VR_05990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HIR6010743.1"
                     /translation="MPKLFIRALTPATRDDEGYHLEAEWLITEDDGSIRANGQTDLRG
                     LAELVDPETDWLQTADNLVVIVPGDYVLGVSCEVPGRSAGQIRRALPFVVEEFVATDI
                     EAMHLAAGEIQRGEPVRCCLVDRSLLEDWLVCLSEIGLSPGHMTAEAELLPAAEGQVS
                     VLLEARRALLKTLDQSATVDRSNLMMALESLTADKLLLVNGDPAALDFELERLEGLTV
                     LTGDDEDAEAGSTLAYLANRWLADGDGVNLLQGAYLPKRPVNPVWARWQGVAALAAAW
                     AGVGIIAMIVEGLWAGAQADRLQAESEALYRDIYPQERRITNVRRQMQAKLGVRAGGE
                     GLGFTEYLAELSAALGQGATVTGLNYNDARDELGADLIVRSYDDAESLQTAIKDRGLN
                     AGITSAEQQEGGVRVRLRVGGA"
     gene            1428..1931
                     /gene="gspM"
                     /locus_tag="AC16VR_05995"
     CDS             1428..1931
                     /gene="gspM"
                     /locus_tag="AC16VR_05995"
                     /inference="COORDINATES: protein motif:HMM:NF016493.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HIR6010744.1"
                     /translation="MLENVNKAFARSSVGQWYAGREPNERVIIGWLVFAVVLTVLWAG
                     VWKPLSDWRAVEDNRYQNAHSLLEWMQANESRARSAAKGQQGNRAQRSLLPTVTTAAQ
                     AEGLVLSRLQPESNGAVSVVLQGQEFNAVIRWLHRLQETNAVSVQRISLDADGRPGYV
                     NAQLRLR"
BASE COUNT          368 a          622 c          709 g          344 t
ORIGIN      
        1 gagagacccg ggccgagctt gcgtccatcg tccatcgcga tcccaacgac ggctcgatga
       61 cgctgatcgc ccgtgatttc ggccgggact tcagatcccg cttcgaggag gaggaaggtt
      121 gaggagcgct gcttccgacc aggaaaacgt atgaagtcaa acaccaagac cggagccaag
      181 taaatgccaa agctgttcat tcgagcgctc acgccggcaa cgcgcgatga cgagggctac
      241 cacctcgagg ccgagtggct gattaccgag gacgacggct ctatccgagc gaacggtcag
      301 accgatctgc gcgggctcgc ggagctcgtc gatccggaga cagactggct ccagacggcg
      361 gacaatctcg tcgtcatcgt ccccggcgat tacgtgctcg gcgtttcctg tgaggtgcct
      421 ggccggagcg cggggcagat ccgccgggcg ctgcccttcg tggtggagga gttcgtcgcc
      481 acggacatcg aggccatgca tctggctgcc ggtgagatcc agcgcggcga gcccgtccgc
      541 tgctgtctgg tggaccgttc tctgctcgag gactggctcg tctgcctttc ggagatcggc
      601 ctctctcccg ggcacatgac cgcggaagcc gagctgctgc cggcggctga gggacaggtg
      661 agcgtgctcc tcgaggctcg gcgggcattg ctgaagacgc tcgatcaatc cgccaccgtc
      721 gaccgctcga atctgatgat ggcgctcgag agtctgacgg cagacaagct gctgctcgtg
      781 aacggcgatc cggccgcact ggatttcgag ctcgaacggc tcgaaggcct caccgtcctg
      841 accggcgatg atgaggacgc ggaagccggt tcaacccttg cttacctcgc gaaccgctgg
      901 ctggccgacg gagacggggt caatctgctg cagggcgctt acctcccgaa aaggcccgtc
      961 aaccccgttt gggcgcgctg gcagggcgtg gccgcgctcg cggccgcctg ggccggcgtg
     1021 ggcatcatcg ccatgatcgt cgaaggtttg tgggccggcg cccaggccga ccggctgcag
     1081 gccgaaagcg aggcccttta cagggacatc tacccccagg agcggcggat caccaatgtg
     1141 cgacggcaga tgcaggcaaa gctcggcgtg cgcgccggcg gagaaggtct cggattcacc
     1201 gagtatctcg ctgagctgtc agcggcattg gggcagggcg cgacggtcac cgggctcaac
     1261 tacaacgatg cacgggatga gctgggcgcc gacctcatcg tgaggagcta cgacgatgcg
     1321 gagtcgcttc agacggcgat caaggatcgt gggctgaacg cgggcatcac gtccgccgag
     1381 cagcaggaag gtggggtgcg agtgcgcctg agagtaggag gagcgtaatg ctggagaacg
     1441 tgaacaaggc cttcgcccgg tcgtcagtcg gacagtggta cgcgggacgc gagcccaacg
     1501 agcgcgtgat cataggctgg ctcgtctttg ccgtggtgct cacggtgctc tgggccggcg
     1561 tctggaagcc gctgagcgac tggcgggcgg tggaggacaa ccgctaccag aacgcccatt
     1621 ccctgctcga gtggatgcag gcgaacgagt cgcgggcacg gtctgccgcc aaggggcagc
     1681 agggcaatcg ggcccagcgc tcgctgctgc cgacggtcac caccgcggcc caggccgaag
     1741 gtctggtgct gagccgcctg cagcccgagt caaacggtgc cgtgagcgtc gtgctgcagg
     1801 gtcaggaatt caacgccgtg atcaggtggc tgcaccggct gcaggagacc aatgctgtca
     1861 gcgttcagcg gatatccctg gacgccgatg gccggcccgg ctacgtgaac gcgcagctgc
     1921 gcttgcggtg aggcatggta cgatcgttca tctcagccgc gaaaaactct gcgctggtaa
     1981 tatgcgccgt gctagttgct ttgattgcag tagagggaat cattcgcctc accgttccag
     2041 ttc
//
LOCUS       DBNHQR010000260         3404 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1292413, whole genome shotgun sequence.
ACCESSION   DBNHQR010000260 DBNHQR010000000
VERSION     DBNHQR010000260.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1292413"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2061
                     /gene="ptsP"
                     /locus_tag="AC16VR_06000"
     CDS             <1..2061
                     /gene="ptsP"
                     /locus_tag="AC16VR_06000"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF008283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HIR6010745.1"
                     /translation="GRIARDRMSLNLADASAHPDFRLFPQSGEEAFPAFLGVPIIHLG
                     AVNGVLIVQDDRPSAFSEDEVSFLVTVAAQLGPILLPLRAPASTSGRRLRGRSGSPGK
                     AVGRVHLVLSEQTVQLVEEPQSAGTEEEIGVLETAVSRTREKIGRAKSQLEAHVAPDV
                     LELFDLYNAMLSGDRLVTAAAGRVREGHSAFSAVRATVDETIAAFEAIEDDYLSARAE
                     DVRHVGNKLLVEILGSAMPSPEHAEGVVLLGGSVGIADVGAFRPEQLAGIVCLQGSSY
                     SHTAVLARALGIPAVVGIGPVDHIVEGMPVIVDGDGGEVIFEPPGVLLRAYEELVASE
                     RQLQNELLAQKDLPGVTTDGCRITLLANTGLMADVRPGKLRGAEGIGLYRSEIPFLMS
                     PVLPTEAEQYATYRELLEIYHPLPVTIRTVDLGGDKQLPYLPTQEENPALGWRGIRFA
                     LDNPAILVSQLRAMLRADHGLGNLQIMLPMISDVSEVQEARRLANTVAAALTAEGLDI
                     RMPPLGVMVEIPAVVDVLPDLAPEIDFACVGSNDLAQYLLAVDRGNPRVVGRYDYLHP
                     GVLGCLDVIARRVEDLGIRLSICGEMAADPVGVVVLLGLGYRALSMNAYSLPRIRALI
                     RGISIREAQALARSALACRNPGSSRVAIAAALAGLGLDHLLAHGGAFESASTPALAGS
                     PSRP"
     gene            complement(2024..2866)
                     /locus_tag="AC16VR_06005"
     CDS             complement(2024..2866)
                     /locus_tag="AC16VR_06005"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR6010746.1"
                     /translation="METELLRTFLEVNRTRHFARAASNLHLTQAAVSARVRHLEEIVG
                     ERLFTRTRNNIQLTVAGHRLLGHAEAIVGSWNRALLETGAPASTRALVALGCLPSIWE
                     TYLTDWLSRTYEDLSPILLQIELLNTEALVRRLRENSITIGLLYEPPQADDLIAEQVT
                     EVSVRLVSSSQGLSVSDSLPGYVYVDWGTSFAIAHDTRLGISTPPALRVDTPAIAYRF
                     LTEHGGTAYLPSAMVAGALSAGLLFPVAGGKTFTRAAFIARSATASAVDSIKAVTASL
                     RALV"
     gene            2987..3247
                     /locus_tag="AC16VR_06010"
     CDS             2987..3247
                     /locus_tag="AC16VR_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010747.1"
                     /translation="MTEIFDEDDDEEGLSPHILEQVRRLQARIDRRESLRREHVARSR
                     GLRRRLEDWEEQRALRSSVDYLEMEFAERDGDRRTGRAALPR"
BASE COUNT          562 a         1075 c         1165 g          602 t
ORIGIN      
        1 ggcaggatcg cccgggaccg gatgtcgctc aacctggccg atgcttcggc ccatcccgac
       61 tttcgtctgt tcccgcagtc cggtgaagag gcgtttcccg ccttcctcgg ggtgccgatc
      121 atccatctgg gcgccgtcaa cggcgtgctc atcgtgcagg acgatcggcc ctcggcgttc
      181 agcgaggacg aggtctcttt cctggtgacc gtagccgccc agcttggacc cattctgctg
      241 ccgctgcgcg cgccggcgtc gacgtccggg cgcaggctgc gcgggcggtc ggggtcgccc
      301 ggcaaggctg tggggcgcgt tcacctggtg ctcagcgagc agaccgtgca gctggtcgag
      361 gaaccgcaat ctgccggcac cgaagaggag atcggcgtcc tggaaactgc cgtgtcgcgg
      421 acccgagaga agatcgggcg cgccaagtcc cagctcgagg cccatgtggc ccccgacgtg
      481 ctggagctct tcgatctgta caacgccatg ctgtccggag accggctggt gaccgccgcg
      541 gcggggcggg tgcgtgaagg gcacagcgcg ttctcggcgg ttcgcgcgac ggtcgatgag
      601 accattgccg cattcgaagc catcgaagac gactacctca gcgcccgtgc cgaggacgtg
      661 cgccatgttg gaaacaagct gctcgtggag atcctgggca gcgccatgcc gtcgcccgag
      721 catgccgagg gcgtcgtgct gctcggcggc tcggtgggga tagccgacgt gggcgcgttc
      781 aggcccgagc agctggccgg aatcgtctgc ctgcagggct cttcgtactc tcacacggcc
      841 gtgcttgccc gggcgctggg catccccgcc gtcgtcggga tcggtccggt tgaccacatc
      901 gtcgagggca tgcccgtgat cgtcgacggc gatggcggcg aggtgatctt cgagcctcct
      961 ggcgtgctgc tgcgggccta tgaggagctc gtcgcctccg aacgtcagct gcagaacgaa
     1021 ctgctggcgc agaaggatct cccgggcgtc acgacggacg ggtgccgaat cactctgctg
     1081 gcgaatacgg ggctcatggc cgatgtccgg ccgggcaagc tgcggggcgc ggaaggcatc
     1141 ggcctgtatc gctcggaaat cccgttcctg atgagccccg tcctgcctac cgaggctgag
     1201 cagtatgcga cctatcgcga gctgctcgag atttatcatc cgttgccggt gaccattcgc
     1261 accgtggacc tcggtggtga caagcagctg ccttacctgc cgacccagga ggagaaccct
     1321 gcgctgggat ggcggggcat tcgcttcgcc ctcgacaatc cggcaatctt ggtctcccaa
     1381 ctgcgcgcca tgctccgcgc cgatcatggc cttggcaatc tgcagatcat gctcccgatg
     1441 atcagcgacg taagcgaagt gcaagaggcg agacgcctgg caaacacggt cgcggcagcg
     1501 ctcacggcgg aaggcctcga catccgcatg cctccgctgg gcgtcatggt cgaaattcct
     1561 gcggtggtcg acgtgctgcc ggacctggcc ccggagatcg acttcgcctg tgtcggttcc
     1621 aacgatctgg ctcagtacct gctcgccgtc gaccgcggca acccccgggt ggtcggccgc
     1681 tacgactacc ttcatcccgg cgtgctcggc tgcctcgacg tgatcgcgcg gcgcgtcgaa
     1741 gatctcggca tccgcttgtc gatctgcggg gagatggcgg ccgaccccgt cggggtggtc
     1801 gtgctgcttg ggctcggcta tcgggcgctg agcatgaacg catacagcct tccgcgtatc
     1861 agggcgctga ttcgaggcat atcgatccgt gaggcacagg cgttggcccg cagtgcgttg
     1921 gcctgccgta atcccgggtc gagccgcgtg gccatcgccg cggcgctggc cgggctcggg
     1981 ctggatcatc tgctggccca tgggggggcg tttgagtcgg cgtctacacc agcgctcgca
     2041 ggctcgccgt cacggccttg atcgaatcga ccgcgctggc ggtcgcgctc ctcgcgatga
     2101 acgcggccct cgtgaacgtc ttccctcctg cgacgggaaa cagcagccct gcggacaggg
     2161 caccggcgac catggccgac ggcagatacg ccgttccgcc gtgctcggtg agaaagcggt
     2221 aggcgatagc cggcgtgtcg acccgcagcg cgggcggggt tgaaatcccc aggcgcgtat
     2281 cgtgcgcgat ggcgaaagac gttccccagt cgacgtaaac gtaaccgggc agagagtccg
     2341 agacgctgag cccctggctg gacgagacca gccttacgct gacctcggtg acctgctcgg
     2401 caatcagatc gtccgcttgg ggcggttcgt agagaagccc gatggtgatg gagttctccc
     2461 gcaggcgcct gacgagcgcc tcggtgttga gcagttcgat ctgcaacaag atcggcgaca
     2521 ggtcctcgta ggtgcggctc agccagtcgg tcagatacgt ttcccagatg ctgggcagac
     2581 aacccagcgc caccagcgcc cgcgtgctcg ccggggcacc ggtttcgagg agggcccggt
     2641 tccaggaccc gacgatcgcc tcggcatgtc cgagcagccg gtgcccggcg acggtcagct
     2701 ggatgttgtt tctggtgcgg gtgaacagcc gctcgccgac gatttcctcg agatggcgca
     2761 cccgcgcgct cacggccgcc tgagtcaggt gcaggttgct ggcggcgcga gcgaagtggc
     2821 gggtgcgatt gacttccagg aaagttctga gcagctcggt ttccatgctt tttgataaac
     2881 atctgttatc aaaaagtaca gaaatatttg atgtatttgc caaatagccg ggcgtatata
     2941 tacgccgacg ttatccgggg cgttgcggcc ccgcatcgag agcgacatga ctgaaatttt
     3001 cgacgaagac gacgacgagg aagggttatc gcctcacatt ctcgagcagg tgcgacggct
     3061 gcaggcccgc atcgaccggc gggaaagctt gaggcgcgag cacgtggcgc gcagccgggg
     3121 actgcggcgc cggctggaag actgggaaga gcagcgggcc cttcgctcga gcgtcgacta
     3181 cctggaaatg gaattcgccg agcgcgacgg agatcgccgc accggccgag ccgccttgcc
     3241 acgctgaccg gctggcccgc acgacttcac ggctagaacg cgccggtgag gttgaggaac
     3301 atgccctgat gatcgaagct cagatccgtc aggtcggacg agaagtccgt gaagttgtag
     3361 ccggcgccga ccttgaagtg atcgccgaag ttgcgcgaga cggc
//
LOCUS       DBNHQR010000261         2870 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_911197, whole genome shotgun sequence.
ACCESSION   DBNHQR010000261 DBNHQR010000000
VERSION     DBNHQR010000261.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_911197"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2141
                     /locus_tag="AC16VR_06015"
     CDS             <1..2141
                     /locus_tag="AC16VR_06015"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HIR6010748.1"
                     /translation="AAHGKGVVHRDLKPDNLFVTRDGRVKILDFGLAKLRQTSDSETH
                     TATLTHSDAPAMEETVAEATEPGRVMGTPSYMAPEQVRGEAVDQRADLFAFGCVLCEM
                     VSGQRPFGRDTSVETMAAILNDEPPDLTEAKPDVPPALARIVHRCLEKLPECRFQSAH
                     DLAFALEATHEQSGPARDAVPAGPAKRRPAMKMFAWPALAAILSVLLVVVGVGWWRTT
                     RTPGRASQIRSLAVLPFTFQTQDPKLSGLGKWIPAEIRSKLGPLQHLQVVNSPARIEH
                     LVQQRKSEVEIARDLAVDGLVMGELHGQGEAISAYVWVVDGATGRALGRQREIAASAA
                     KLSEIPNQVALAIVDELKLQVSAGQRAEIQLADTQNAEAFLAYQKGRDLLGSRRFGEA
                     AVELRRANQLDPDYTRAWAALASTEWNPLIYGGTTREMTATFKRLSEEAERFGAQRPD
                     DPAVATLRMWMAMIYERDWKKVSAIFWELARGQKPDPGVVHTMSWYYTLIEGHPELAS
                     DGSRQAIAMAPETLIYQIDRAHQLSFIGRPADAIGAFRSLPPERIQLEDYSGALLMAG
                     DPAGAREVALRVLAGFPNPWTRCLLAGIEAELGAADEARKILAQLEADAEKGMHIPYA
                     RIAWSYGMVGDFEAARRWLGKGLLEGHGDWTMLELRSATMLEMFGKLAWYWDIVDGME
                     FPPLQMDNPYFALEQAMRYGRGDQSPAARP"
     gene            complement(2214..>2870)
                     /locus_tag="AC16VR_06020"
     CDS             complement(2214..>2870)
                     /locus_tag="AC16VR_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR6010749.1"
                     /translation="PTATLPLWSPDQTMLYFGDRRSSTYEAWTALLDGSRAAERLFEN
                     PVPSTLPTSVAEDGTLMGYGVHPVTNRDIWIAPPGEKPTVLLQTPANERAPAIAPFGR
                     LYAYVSNEEGTDEIYLRDLESPERRWQLSNGGGISPVWGRDGRELFFLRGSSLIGVEV
                     QTTGGVRIGKERVVFSHDRLLLDDWGNRTFDTLPEGGFLVSVQRESEVVLRVVLGFGG
                     "
BASE COUNT          500 a          895 c         1021 g          454 t
ORIGIN      
        1 cagccgcgca tggcaagggc gttgtccacc gcgacctgaa gccggacaac ctgttcgtca
       61 cgcgagacgg ccgggtgaag atcttggact tcggattggc gaagctccgg caaacttcgg
      121 actccgagac ccataccgca acgctgaccc attccgacgc accggctatg gaggaaaccg
      181 tcgcagaggc cacggaaccg ggccgggtga tgggcacgcc cagctacatg gcaccggagc
      241 aggtgcgggg cgaggcggtg gaccagcggg cggacctctt cgcgttcggg tgcgtgcttt
      301 gcgagatggt gagtgggcag cggccgttcg ggcgcgacac gtccgtcgaa acgatggcgg
      361 cgatcctgaa cgacgagccg cccgacctga cggaggcgaa gccggacgtg ccgccggcgc
      421 tggctcggat tgtccaccgg tgtttggaaa aactgccgga gtgccggttt cagtcggcgc
      481 acgatctggc gtttgcgctg gaagccaccc acgagcagag cgggccggcc cgtgatgccg
      541 tcccggcagg gcctgcgaag aggcgtccgg cgatgaagat gttcgcgtgg cccgcgctcg
      601 cagccatctt gtcggtcttg ctggtcgtcg tcggcgtggg ttggtggcgg acgacccgca
      661 cgccgggccg cgccagccaa atccgctcgc tggcggtgct tcccttcacg ttccagacgc
      721 aagatccgaa gctctccgga ctggggaaat ggattccggc ggagatccgc tcgaagctcg
      781 ggccgttgca gcatttgcaa gtggtgaact cgccggcccg gatcgagcat ttggtgcagc
      841 agaggaaatc ggaagtggag atcgcgcgcg acctggccgt ggacggcctg gtgatgggcg
      901 aattgcacgg ccagggggag gcgatatccg cctacgtctg ggtggtggac ggtgcgaccg
      961 ggcgtgcgct gggccggcaa cgggagatcg ctgcttcggc tgccaagctc tccgagattc
     1021 cgaaccaggt ggcgttggcg atcgtggatg aattgaagct gcaagtcagc gccggacaga
     1081 gggccgagat tcagctggcg gatacccaga atgccgaggc ctttctggct taccagaagg
     1141 ggagagatct gctcggcagc cgtcggtttg gcgaggcggc ggttgagctg cgacgggcga
     1201 atcagctcga tccggactac acccgggcgt gggcagccct ggcgagcacg gaatggaatc
     1261 ccctgatcta tgggggaacg acccgcgaga tgaccgcgac cttcaagcgg ctatcggagg
     1321 aagcagaacg gttcggcgca caacggcccg atgatccggc tgtcgccacg cttcgcatgt
     1381 ggatggccat gatctacgag cgggactgga agaaggtgag cgcaatcttc tgggaactcg
     1441 cgcgcggcca gaagccggac cccggggtgg tgcataccat gtcatggtat tacaccctga
     1501 tcgaagggca tccggagctg gcatcggacg gcagccggca ggccatcgcg atggctccgg
     1561 agactctcat ctatcagatc gatcgtgcgc accagctctc cttcattggc cgacccgccg
     1621 acgcgatcgg cgccttccgc agccttccac ccgagaggat ccagctcgag gattattccg
     1681 gcgcattgct gatggcagga gatccggccg gcgccaggga ggtggcgctt cgcgtgctcg
     1741 caggattccc gaatccatgg acgcggtgcc tgctggccgg catcgaggcc gagttgggcg
     1801 ctgcggatga ggcccgcaag atcctggcgc aattggaggc ggatgcggag aagggcatgc
     1861 acatccccta tgcgcgtatc gcgtggagct acggcatggt cggcgacttc gaggccgccc
     1921 gccgttggct gggcaaagga ctgctggaag gccacggaga ctggaccatg ctcgagctgc
     1981 gctcggcgac gatgctggag atgttcggca agctcgcgtg gtactgggac atcgtggatg
     2041 gcatggagtt tccgccgctc cagatggata atccatactt cgcactggag caggccatgc
     2101 gctatggccg gggcgatcaa tcacctgcag cgcggccctg aacatcgccg gcgtcggctg
     2161 aggaactcgg atccatccgg cgctacccgg tcgagccaat ggccggggaa gggctaaccg
     2221 ccgaagccca gcaccacgcg caagacgacc tccgactccc gctgcaccga gaccaggaac
     2281 ccaccctccg gcaaggtgtc gaaggtccgg ttcccccaat cgtccaggag cagccggtcg
     2341 tgcgagaaga cgacgcgctc cttgccgatc cgcaccccgc ccgtcgtctg cacctcgacg
     2401 ccgatcagcg agctgccgcg caggaagaag agctcgcgcc cgtcgcgccc ccagaccggc
     2461 gagatgccgc cgccgttcga gagctgccag cgccgctccg gcgactcgag gtcgcgcagg
     2521 tagatctcgt cggtgccctc ttcgttggag acgtaggcgt acaggcggcc gaagggtgcg
     2581 atcgccggcg cccgctcgtt ggccggcgtc tgcagcagca ccgtgggctt ctcaccggga
     2641 ggcgcgatcc agatgtcccg gttggtcacc gggtgcacgc cgtaccccat cagcgtgccg
     2701 tcctcggcga ccgaggtcgg cagcgtcgac gggaccgggt tctcgaagag gcgctcggcg
     2761 gcgcggctgc cgtccagcag cgcggtccag gcctcgtagg tcgagctacg ccggtcgccg
     2821 aagtagagca tcgtctggtc gggagaccag agcgggaggg tcgcggtcgg
//
LOCUS       DBNHQR010000262         2441 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1734586, whole genome shotgun sequence.
ACCESSION   DBNHQR010000262 DBNHQR010000000
VERSION     DBNHQR010000262.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1734586"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="AC16VR_06025"
     CDS             <1..1077
                     /locus_tag="AC16VR_06025"
                     /inference="COORDINATES: protein motif:HMM:NF012364.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HIR6010750.1"
                     /translation="RNVMVFDFKSLYPSIIRTFNVDPLGFVPSGDAEPDVIRLVNGAG
                     FRRDPAILPRMLDDLFPRREAAKTAGDSVASQAIKILMNSFYGVLGTPACRFHNVEIA
                     NAITGQGRHLLQWSKRWFETRGFDVLYGDTDSLFVRSRQGDEDAALAEARDLAARLNA
                     DIAAEVLERWGVVSRLELEFEKLYAKLFLPSVRHGAAGARKRYAGLPVGAEEVEFVGM
                     EVVRRDWTELAKRVQRELYARLFSGAAVDDYLAGVVAQLRRGELDELLVYRKGLRKRL
                     DSYTASNPPHVAAARKSGEPPGRTVAYLVTSAGPEPLDALQHEPDREHYVDKQIKPVA
                     EPVLGALGLEFARVIGDDRQMGLF"
     gene            complement(1108..2148)
                     /locus_tag="AC16VR_06030"
     CDS             complement(1108..2148)
                     /locus_tag="AC16VR_06030"
                     /inference="COORDINATES: protein motif:HMM:NF015715.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HIR6010751.1"
                     /translation="MADVPTPASRLARYRTDYLLMTCAAIALVLLILALTGAAPMGTP
                     LGHAAHTVHEMLAAMWWGIALAILIIGVLERIPQEFIIALLGPPGSRNGVLRAAVAGV
                     LLDLCSHGILAVGAKLYQRGASAGQVMAFLIASPWNSVSLTFILVGLIGLGWTIAFIG
                     LSLVVAITTGLAFDRLARTGRIPANDAQVDLPVNFRFGPASRELVSTIEWEPRLLADI
                     VINGLRGTRIVLRWLMFGLVVAAALRAFLSPEDFAQWFGPSLLGLGLTLVGATIFEVC
                     SEGSVPIAADLMNRAGAPGNSFTFLLAGVASDYTEIMVLREATGSTKIALMLPAICVP
                     QVLLIGWVLNQL"
     gene            complement(2235..>2441)
                     /locus_tag="AC16VR_06035"
     CDS             complement(2235..>2441)
                     /locus_tag="AC16VR_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010752.1"
                     /translation="HQAELNLRDNELQQAFTGVHDGPFEPDAAEVAEVRAVGLQELAA
                     EIERWPARFTPWFVRDLAELGFVK"
BASE COUNT          431 a          759 c          856 g          395 t
ORIGIN      
        1 cgaaacgtca tggtctttga tttcaaaagc ctctacccga gcatcatccg aactttcaac
       61 gtcgatcctc tggggttcgt gccatcgggc gacgcggagc ctgacgtgat ccggctcgtt
      121 aacggcgctg gattccggcg ggatccggcc atcctgccgc gcatgctcga tgacctgttt
      181 ccgcgccggg aggctgcgaa gacagctggg gacagcgttg cctcacaggc gatcaagatc
      241 ctcatgaact ctttctacgg tgtgctaggc accccggcct gtcggtttca caacgtcgag
      301 atcgcaaacg ccatcaccgg tcagggccgc catctgctgc agtggtcgaa acgctggttc
      361 gagacgaggg gcttcgacgt gctctacggc gacaccgaca gccttttcgt gcggtcgcgg
      421 cagggcgacg aggatgctgc ccttgcggaa gcgagggatc tcgccgcgcg gctcaatgcg
      481 gacattgccg ccgaggtgct cgagcgctgg ggggtcgtca gccgcctcga gctggaattt
      541 gaaaagctct acgccaagct cttcctgccg tctgttcgtc acggtgcggc gggcgcgcga
      601 aagcgctatg cggggttgcc tgtcggggcc gaagaggtgg agttcgtggg catggaggtc
      661 gtccgccggg actggaccga gcttgccaag cgtgttcagc gggagctgta tgccaggctc
      721 ttctccggag ccgccgtgga tgactacctg gccggagtcg tcgcgcagct gcgtcgcggc
      781 gagctggacg agcttctggt ctatcgcaaa gggctcagga agcgactgga cagctacacg
      841 gcgagcaacc ctccccacgt ggctgcggcg cgaaagtcgg gagagccgcc tgggcgcacc
      901 gtggcttacc ttgtgaccag cgcgggcccc gagcctctcg acgcgctgca gcatgagccc
      961 gatcgggagc attacgtcga caagcagata aagccggtgg ccgagcccgt tctcggggcg
     1021 ctgggtctgg aattcgcccg ggtgatcggc gacgaccggc agatggggct gttctgaagg
     1081 ggagggctcc cgcccgccgg cacctggtca gagctggttc agcacccagc cgatcagcag
     1141 aacctgggga acgcagatgg cgggcagcat cagggcaatc ttcgtcgatc cggtggcttc
     1201 gcgcaggacc atgatctcgg tgtagtcgct ggccacgccg gcgagcagga aggtaaagct
     1261 gtttcccggg gcccccgctc ggttcatcag atccgcggcg atgggcacgg agccctcgga
     1321 gcagacctcg aagatggtcg cgccgacgag ggtgaggccc aggcctagca ggcttggtcc
     1381 gaaccactgc gcgaagtcct cggggctgag aaaggccctc aacgcggcag cgacgactag
     1441 cccgaacatc agccagcgga gcacgatacg ggtcccgcgc agtccgttga tgacgatgtc
     1501 cgcgagcagg cgaggctccc actcgatggt gctgaccagc tcacgggaag ccggaccgaa
     1561 acggaagttc accggaagat cgacctgggc gtcgttagcc gggattcgtc ccgtgcgggc
     1621 caggcggtcg aacgctaggc cggtggtgat ggcaaccacc agcgacaggc cgatgaaggc
     1681 gatggtccag ccgagcccga tgaggccgac gaggatgaag gtcagagaaa cggagttcca
     1741 cgggctggcg atgagaaatg ccatgacctg cccggcgctt gcgccccgct ggtagagctt
     1801 ggccccgacg gcgaggatcc cgtggctgca caggtcgagc agcacgccgg cgacagcggc
     1861 ccgcagaacg ccgttccggc ttccgggagg tccgagcagc gcgatgatga actcctgggg
     1921 tatccgctcc aggacgccga tgatgaggat ggccagcgcg atgccccacc acatggccgc
     1981 gagcatctcg tgcacggtgt gagctgcatg gccgagcggg gtccccatgg gcgcggcgcc
     2041 cgtcagcgcc aggatcagaa gaaccagcgc gatggccgcg caggtcatca gcaggtaatc
     2101 ggtgcggtat ctggccagcc ggctggcggg cgtcggcacg tcagccactt gggcgtccca
     2161 tcgagcgaag agggttgagg ctacgcctgg tcctgtcctg ctggcaagtg cggttgctcg
     2221 acaagtcgag gcgcctactt cacgaagccc agctccgcga ggtccctgac aaaccagggg
     2281 gtgaagcgag cgggccaccg ttcgatttcc gcggcgagct cctgaaggcc gacggcgcgg
     2341 acctcggcga cctcggcggc atccggctcg aaggggccgt cgtgcacgcc ggtgaacgcc
     2401 tgctggagct cgttgtcccg caagttcagc tccgcctgat g
//
LOCUS       DBNHQR010000263         4216 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1734884, whole genome shotgun sequence.
ACCESSION   DBNHQR010000263 DBNHQR010000000
VERSION     DBNHQR010000263.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1734884"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="AC16VR_06040"
     CDS             complement(<1..258)
                     /locus_tag="AC16VR_06040"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6010753.1"
                     /translation="MASRELVDSYNDAVMRFDGDAWAANWRDDATWSLPGAGPIKGKE
                     NFFPMWQQAMSAFSFVGFFASAGPIVVDGDTAHATWYQQEFL"
     gene            complement(386..1153)
                     /locus_tag="AC16VR_06045"
     CDS             complement(386..1153)
                     /locus_tag="AC16VR_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6010754.1"
                     /translation="MIDVYYWPTPNGWKVTIMLEECGLPYRILPVDIGGGDQFTPDFL
                     RVSPNNRMPAIVDHEPLGGGEPLSIFESGAILEYLADKSGKFLSREPAGRYRTLQWVH
                     WQMANLGPMMGNANHFKNYGKNIAEDPQQLEYGTRRFVGEVDRLSGVMDAQLRVNRYL
                     AGSEYTIADIITWPWAMLLGRLIDEAVWETFPNLKRWVDEVGERPGVEKGRKVERDLG
                     RQERTEEQEKARRELLFNQTNEKVRVAREAAAKAAAG"
     gene            1315..2523
                     /locus_tag="AC16VR_06050"
     CDS             1315..2523
                     /locus_tag="AC16VR_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010755.1"
                     /translation="MLNVETLRVTLVAAMVLLAAQPAHADGTYGADFRAHLLPEAGTA
                     HASITITQEAGELKRLDLAAPAATYSAFSGPGSIRREEDRLVWTVPEDGGTLSYDVLI
                     DHRRNGAYDARMTPDWAIARLDDLFPRARTRSESGAQSRSSVVLAGPAGWRFETRYGP
                     SHEQVAVVDEGRRFDRPTGWLVAGRLGIRRDTIAGRTVSVAAPVGQGFRRVDVLAFLR
                     WTLPDLVAVFPGMPDRLLVVGAPEGMWRGGLSGPESLYLHVGRPLISENGTSTLLHEL
                     VHIATTAPKSAGDDWIMEGLAEYYSLETLRRSGGISEERFASALGSLAAWAQRDGGRL
                     ADPSTGAHTARAALTFHELAAELEDAGSSLDAVVGELVAGGSLDRGSLLALLEPKLTE
                     PSGILAELPR"
     gene            complement(2551..4098)
                     /locus_tag="AC16VR_06055"
     CDS             complement(2551..4098)
                     /locus_tag="AC16VR_06055"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6010756.1"
                     /translation="MYPGKHVTEHAERPAIIMASTGEVITYKELEARTNQLAHLLRDQ
                     GLKRLDHYAIFMENNSRYVEACGAGERSGLYYTCINSYLTPEEVAYIVNNSESRVLIT
                     SREKAEVAKAALADCPRVALCLVVDGDDDARVVNYESAIERFPSTPIPDESLGTAMLY
                     SSGTTGRPKGIIRPLPEAPPEQDLPLFQFINGIWRFREGMIYLSPAPLYHSAPQGSVN
                     LTIRRGGTAVIMERFDPEQFLELVERYRITHSQLVPTMFSRMLKLPEEIRARADVSSL
                     EIAIHAAAPCPVQVKEAMIDWWGPIIYEYYGATEGLGFTGCTTEEWLAHKGTVGRVLL
                     GELHILDEDGNPSPKGEPGEVWFKTASAFEYFNDPEKTAQATSADGSMTTVGDVGYVD
                     DDGYLYLTDRATFMIISGGVNVYPQECENLLITHPKVADAAVFGVPNVDLGEEVKAVV
                     QVMPDVEPGEALEAELIAFCHEHLARQKCPRSVDFEEQLPRLPTGKLYKRHLRDRYWG
                     DKKSRIV"
BASE COUNT          645 a         1348 c         1421 g          802 t
ORIGIN      
        1 gagaaactcc tgctggtacc aggtggcgtg agcggtgtcg ccatccacca cgataggccc
       61 ggccgaggcg aagaagccga cgaaggagaa cgcgctcatg gcctgctgcc acatgggaaa
      121 gaagttctct tttcccttga tcggtccggc cccgggcaga gaccaggtgg cgtcatcgcg
      181 ccagttggcc gcccacgcat ctccatcgaa acgcatgacg gcatcgttgt acgagtcgac
      241 tagctcgcga cttgccatgc ggtcttccag tggtccactg ctgatcattt gcttccctcc
      301 cttcttggtc ttgaatgcgg tctcgaagaa tcacacttaa tcacgacgcg ctgcgcattg
      361 cgagcgcgtg gcgaggtccg gcccgtcagc cggcggccgc tttcgccgcg gcctctcgtg
      421 ccacacgcac tttttcgttg gtctggttga acaggagctc ccgccgggct ttctcctgtt
      481 cctcggtccg ctcctggcgg ccgagatcgc gttccacttt gcggcccttt tccacgccgg
      541 ggcgttcgcc cacctcgtcc acccagcgct tgaggttcgg aaaggtctcc cagacggcct
      601 cgtcgatgag gcggccgagc agcatggccc acggccaggt gatgatgtcc gcgatggtgt
      661 actcgctgcc ggcgagatac cggttgacgc ggagctgagc gtccatcacg ccggacagac
      721 ggtccacttc gcccacgaaa cgacgggtgc cgtattccag ctgctgcggg tcctcggcga
      781 tgttcttgcc gtagttcttg aagtgattgg cgttgcccat catcgggccc agattggcca
      841 tctgccagtg aacccactgc agggtgcggt atcggccggc cggctcccgg gacagaaact
      901 tccccgactt gtcggcgaga tactcgagta tggcgccgga ttcgaagatc gacagcggct
      961 ccccgccgcc gagaggctcg tgatcgacga tggccggcat gcggttgttg gggctcacgc
     1021 gcagaaagtc cggcgtgaac tggtcgccgc cgccgatgtc gaccggcagt atccggtagg
     1081 gcaggccgca ctcctccagc atgatggtga ccttccagcc gttcggggta ggccagtaat
     1141 acacgtcgat catcgcttcg ctcctctcag cgccagtcgc gtccgcgcgt acactatggg
     1201 agattcaatt tctcgattca aacgtcggcg cgcttccaat cccgacggcg ctctggtatc
     1261 tgcgctgtgt catccagccg ttatcgcgaa ggtgccgtgt ggagtcgtca aacgatgctg
     1321 aacgttgaga ccctgagggt caccctggtg gcggcgatgg tgctgctcgc cgctcaaccc
     1381 gctcacgccg acgggactta cggcgctgat ttcagggcgc acctgcttcc cgaagcggga
     1441 acggcgcatg cctcgatcac cattactcag gaggcgggcg agctcaagcg cctggacctg
     1501 gcggccccgg cggcgaccta ttcggccttt tcaggtcccg ggagcatccg gcgcgaggaa
     1561 gatcgtctgg tctggaccgt tcctgaggac ggcggcaccc tgagctacga cgtgttgatc
     1621 gaccatcgcc gcaacggggc ctacgatgcg cggatgacgc cggattgggc catcgcgcgg
     1681 ctggacgatc tctttccgcg cgcccggacg cgcagcgagt cgggggcgca atcccggtcg
     1741 agcgtcgttc ttgccggacc ggcgggctgg cggttcgaga cccgctacgg gccgagccac
     1801 gagcaggtgg ccgtggtgga cgaggggcgc cggttcgacc ggcccaccgg ctggctggtg
     1861 gccggcaggc tcggcatccg cagggatacc atcgccggcc ggacggtttc agtggcggcg
     1921 ccggtggggc agggctttcg ccgggtggac gtgctcgcct tcctgcgctg gacgctgccc
     1981 gatctcgtcg ccgtgtttcc cggaatgccc gaccggctgc tcgtcgtagg tgccccggag
     2041 ggcatgtggc gagggggcct ttccggcccg gaatcgctgt acctccacgt cgggcgcccg
     2101 ctcatcagcg agaacggcac cagcacgctg cttcatgagc tcgtgcacat cgccacgacg
     2161 gcgccgaaat cggctggcga cgactggatc atggaagggc ttgccgagta ctacagcctg
     2221 gagacccttc ggcgcagcgg gggtatcagc gaggagcgct tcgcgtcggc cctggggtca
     2281 ctggcggcct gggcgcaacg tgacggcggg cgcctggcgg acccatccac gggtgcgcac
     2341 acggcccgcg cggcattgac gtttcatgag ctcgctgcgg agctcgagga tgcgggcagc
     2401 agcctcgacg ccgttgtggg agagctggtc gcaggcggca gccttgatcg cggcagcctg
     2461 ctggcgttgc tggagccgaa gctcaccgag ccatcaggaa ttcttgccga actcccccgt
     2521 tgaggcgggc gcccgcctgc ctgtttcagc tcagacgatg cggctttttt tgtcgcccca
     2581 gtaccggtcg cgcaggtgac gcttataaag cttgcccgta ggcaggcggg gcagctgttc
     2641 ctcgaagtcg acggagcggg ggcacttctg gcgggcgagg tgctcgtggc agaaggcgat
     2701 gagctccgct tccagcgctt cccccggttc cacgtccggc atgacctgga ccacggcttt
     2761 cacctcttcg ccgagatcca cgttgggcac gccgaacacc gcggcgtcgg caaccttcgg
     2821 atgggtgatc agcaggttct cgcattcctg gggatagacg ttcaccccgc cggagatgat
     2881 catgaacgtc gcccggtcgg tgaggtacag gtagccgtcg tcatccacgt agccgacgtc
     2941 gcccacggtg gtcatgctgc cgtctgccga ggtggcctgc gccgtcttct cgggatcgtt
     3001 gaagtactcg aaggccgacg cggtcttgaa ccagacctcg cctggctcgc ctttcgggga
     3061 agggttgcca tcctcgtcga ggatgtgcag ctctcccagc agcacccggc ccacggtgcc
     3121 cttgtgcgca agccactcct cggtggtgca gcccgtgaag cccaggcctt ccgttgcgcc
     3181 gtagtactcg tagatgatgg ggccccacca gtcgatcatc gcttccttga cctgcaccgg
     3241 gcatggcgct gccgcgtgga tggcgatctc cagggacgag acatcggccc gggcgcggat
     3301 ctcctcgggc agcttcagca tgcggctgaa catcgtcggc acgagctggc tgtgggttat
     3361 gcggtaacgt tcgaccagtt ccaggaactg ctcggggtcg aagcgctcca tgatcacggc
     3421 ggtgccgccc cgccggatgg ttaggttgac cgagccctgg ggggccgagt ggtagagggg
     3481 tgcgggcgag aggtagatca tgccttcgcg aaagcgccag atgccgttga tgaactggaa
     3541 gagcggcaga tcctgctccg gcggtgcttc cggcaggggc cggatgatgc ccttgggacg
     3601 cccggtggtg ccggaggagt agagcatggc cgtgccgagg ctctcgtcgg gaatcggcgt
     3661 cgaaggaaat ctttcgattg cgctttcgta gttgaccacc ctggcgtcgt cgtcgccgtc
     3721 gacgaccagg cacagcgcta cccgggggca gtccgcgaga gccgccttcg ccacctccgc
     3781 cttctccctg gaagtgatga gaacgcggga ttcgctgttg ttgacgatgt aggccacttc
     3841 ctccggcgtg aggtacgagt tgatgcacgt gtagtagagg cccgatcgct cgccggcgcc
     3901 gcaggcttcc acgtagcggc tgttgttctc catgaagatg gcgtagtggt cgagcctttt
     3961 gaggccttgg tcgcgcagga gatgggccag ctggttggta cgtgcttcga gctccttgta
     4021 cgtgatgacc tctccggtcg aggccatgat gatggcgggc ctttcggcgt gctccgtgac
     4081 gtgcttgccc gggtacatga cgaacctccc tcgacggcta gcgcgggcgg gcgccgccgc
     4141 ccacgcgaat gatctcgccg ctcacccagc tggccgcgtc cgaggccagg tagacgcagg
     4201 cagcgccgat gtcctg
//
LOCUS       DBNHQR010000264         2153 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1609097, whole genome shotgun sequence.
ACCESSION   DBNHQR010000264 DBNHQR010000000
VERSION     DBNHQR010000264.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1609097"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..478
                     /locus_tag="AC16VR_06060"
     CDS             164..478
                     /locus_tag="AC16VR_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010757.1"
                     /translation="MNHPVTQRSLRQQNQAFSGTNGVSQNNRSMRFLPAFRDEETGQV
                     ELARFDDGRVAPAHLIVGLPRRWAVAFASDGAILALKPSITAGFVRDDVFFTREEAAS
                     AD"
     gene            complement(475..1695)
                     /locus_tag="AC16VR_06065"
     CDS             complement(475..1695)
                     /locus_tag="AC16VR_06065"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6010758.1"
                     /translation="MAAPLEDITVVAIESWMAAPSASALLADLGANVIKVEPPSGDPM
                     RNLSRAPKVDGPLKALDCQFDVDNRGKRSVAVDLDTNQGKAVVHRLVARADVFMCNLL
                     IHRQERFGLDPDSVLALNPRIVHATLTGYGTTGPDAWRPGYDVTAFFGRSGLYDASRE
                     GDDGDVPMARPAQGDHTTGLALLGAILAALRSAERTGEGQVVETSLFETAIWTQASDF
                     GTTAIDRAPVRRRARHNMLSPTANRYPCGDGKWLVVNMLDAMAFDRFCRAIGREDWLE
                     DERFREARGRYDHMPELVTGIDATLATRSRDEWGRIFDEAGLIWGPVLTLDEAAGDGQ
                     AHAIGMFPELEHPEYGTYRTVNAPFRFRSGEVRPRGTSPGPGEHTREVLGEMDFDEAE
                     IDALMAAGVLPTHD"
     gene            1818..>2153
                     /locus_tag="AC16VR_06070"
     CDS             1818..>2153
                     /locus_tag="AC16VR_06070"
                     /inference="COORDINATES: protein motif:HMM:NF021438.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HIR6010759.1"
                     /translation="MRELHYLRGPWAAAASILMLVTGVLWTPVASANQTVDTDAKASR
                     VEGVWASKGAVFAIERSGDTLQATVVALRKPRPDRKNAKAELRDRPLIGLQLLSDYRF
                     KRGRWRGDFY"
BASE COUNT          354 a          715 c          710 g          374 t
ORIGIN      
        1 ggtgttatgg agcctcggct gaaattctgg agtttggcat ggttgatgct aaacgaaacg
       61 gtaggtaacg aatgagcgaa tctgaaacgc aactgccatt ccagctgagg tctcgactgg
      121 catgtgcgga gcttcaggga agttgtagga gcttcacgtc gtcatgaacc atccggtcac
      181 ccaaaggtcg ctgcgccagc agaaccaggc cttctccggc accaacggtg tcagccagaa
      241 caaccgctcg atgcgtttcc tcccggcctt ccgcgacgag gaaaccggac aggtggagct
      301 ggcgcgcttc gacgacggca gggtcgcgcc cgcgcacctc atcgtcgggc tccctcgtcg
      361 atgggcggtc gcatttgcga gcgacggtgc cattctggcg ctcaaaccga gcatcaccgc
      421 cgggttcgtc cgcgacgacg tcttcttcac ccgcgaggaa gccgcctctg cggactagtc
      481 gtgcgtcggc aacaccccgg ccgccatcaa cgcgtcgatc tccgcctcgt cgaaatccat
      541 ctcgccgagc acttctctgg tgtgctcacc gggacccggt gacgttcctc tcggcctgac
      601 ctcccccgat cggaatcgga acggcgcgtt gaccgtccgg taggtgccgt actccgggtg
      661 ctcgagctcg gggaacatgc cgatggcgtg agcctggccg tcgccggcgg cctcgtcgag
      721 ggtcagcacc ggcccccaga tcaggccggc ttcgtcgaag atccggcccc actcatcgcg
      781 gctgcgggtc gccaaggtcg catctatgcc cgtaaccagc tcgggcatgt ggtcgtagcg
      841 gccgcgggct tcacggaaac gctcgtcctc cagccagtct tctctgccga ttgcccggca
      901 gaagcggtcg aacgccatgg catccagcat gttcaccacc agccacttgc cgtcgccgca
      961 cggatagcgg tttgcggtcg gggagagcat gttgtgacgc gcacgccgac gcacgggggc
     1021 acggtcgatg gccgtggtgc cgaaatccga ggcctgggtc cagatggccg tctcgaacag
     1081 agaagtttcc accacctggc cctcgcccgt gcgctcggcc gatcgcaacg cggcgaggat
     1141 ggccccgagc agcgccaggc cggtggtgtg gtcgccctgg gccgggcgcg ccatgggcac
     1201 gtctccgtcg tcgccctcgc ggctggcatc gtagagaccc gagcggccga agaaggcggt
     1261 cacgtcgtag ccggggcgcc aggcgtctgg cccggtggtg ccgtagccgg tcagcgtggc
     1321 gtgcacgatg cgcgggttca gcgccaggac gctgtcggga tcgagcccga aacgttcctg
     1381 ccgatgaatg agcaggttgc acatgaacac gtcggcccgg gcgacgaggc gatgcacgac
     1441 ggccttgccc tggttggtgt ccaggtccac ggccaccgat cgcttgccgc ggttgtccac
     1501 gtcgaactga cagtccagcg ctttgagcgg accgtcgacc ttgggcgcgc gcgagagatt
     1561 gcgcatggga tcgccgctcg gcggctcgac cttgatcacg tttgcgccga ggtctgccag
     1621 cagggcgctc gcgctcggcg ccgccatcca gctctcgatg gcgacgacgg tgatatcttc
     1681 cagcggtgca gccaacggct cgcctcctct ttgagctcac ggggcgtgaa agcccggttg
     1741 taccgcatat tcgcggcaaa ttcagcttcc gcgcgagaac tggcagtagc ttcggcggcg
     1801 cggtaacctt gcgcgccatg cgggaactgc actatctgcg agggccgtgg gcggcggctg
     1861 cttcgatcct catgctcgtg acgggcgtgc tctggacacc cgtcgcctcg gccaaccaaa
     1921 ccgtagacac ggacgccaaa gccagccgcg tagagggggt ctgggcctcc aagggtgccg
     1981 tgtttgccat cgagcgctcc ggggacaccc tgcaagccac cgtcgtcgct ctgcgcaaac
     2041 cccgacccga ccgcaaaaac gctaaagccg agctgcgcga ccggccgctg atcgggctgc
     2101 agctgttgag cgactatcgt ttcaagaggg gccgctggcg cggggatttc tac
//
LOCUS       DBNHQR010000265         2081 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1039176, whole genome shotgun sequence.
ACCESSION   DBNHQR010000265 DBNHQR010000000
VERSION     DBNHQR010000265.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1039176"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="AC16VR_06075"
     CDS             complement(<1..517)
                     /locus_tag="AC16VR_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010760.1"
                     /translation="MAAIIIAVLKTNDCGSPRFGCRVRTGNGGNLAGSINQTALLRNL
                     SLITLCLVLAGCDGQETTARPGPSGAWGGPTTVVTVAAVKRPLRTRVEALGTAVANES
                     VTITAQVTDSVSQVRFEDGDYVNAGDVLVELTSTEESAQLDEARARHSDAKQRYDRVV
                     DLAREGTESQAR"
     gene            507..2015
                     /locus_tag="AC16VR_06080"
     CDS             507..2015
                     /locus_tag="AC16VR_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007351815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6010761.1"
                     /translation="MAATLTQRIVESFERNAERPCLRVPGGRDWSYGELNRLAEKLAG
                     LLAGVPVAGGDRVLVQVEKSPEAVALYLACLRLGAVYVPINTAYTGSEVEYFLRDAEP
                     SLFVCRPSVQPALAEIAGSVPVLTLGADGDGSLLEGALKAEARDEAEPRDPADLAAIL
                     YTSGTTGRSKGAMLSQGNLLSNAESLLECWVWRDDDVLLHALPIFHVHGLFIALHCAF
                     LSGTPVTFLAKFDPEAIVECLPESTVLMGVPTFYTRLLDFPGFGADVCRNMRLFISGS
                     APLTEATFHAWRERTGFEILERYGMSETIIIASNPLNGERLAGTVGFALPGMEVRIAD
                     EEGKPLSGDDVGMIEVRGSSVFSGYWRMPEKTAEEIRPDGFFVTGDLGRMDADGRVTI
                     VGRSKDLVISGGYNVYPKEVERLIDELPGVVECAVIGVPHPDFGEAVVAVVVPENEEV
                     SERSVAAGLDGQLARFKQPKRVINIGELPRNAMGKVQKNELRERFADLFAQS"
BASE COUNT          331 a          652 c          709 g          389 t
ORIGIN      
        1 ggcgggcctg gctctcggtg ccctcgcggg cgaggtccac cacgcggtcg tagcgctgct
       61 tcgcatccga atggcgggcg cgggcctcgt cgagctgggc cgactcctcc gtgctggtga
      121 gctccaccag cacgtcgccc gcgttgacgt agtcgccgtc ctcgaaacgc acctggctga
      181 cgctgtccgt gacctgggcg gttatcgtga ccgattcgtt tgccaccgcc gtgccgagcg
      241 cctctacccg ggttcgcagg ggccgtttca cggccgcgac cgtgaccacg gtggtcgggc
      301 cgccccaggc cccggaaggg cctggacggg ccgtggtttc ctgcccgtcg catcctgcaa
      361 gaacgaggca caaagtgatg agggagaggt ttctcaaaag cgccgtctgg ttgatcgagc
      421 cggcaaggtt accgccattg ccggtacgaa cgcgacatcc aaagcggggg ctgccacaat
      481 cgttcgtttt caggacggcg atgatgatgg cggccaccct cactcagcgg atcgtggagt
      541 cgttcgagcg aaatgccgaa cgtccctgcc tgcgtgttcc cggcgggcgc gactggtcgt
      601 atggtgagct caaccgactg gcggagaagc tggccggcct gcttgccggc gtgccggtgg
      661 cggggggaga ccgggttctg gtccaggtgg aaaagtcccc tgaggcggtc gccctctacc
      721 tcgcctgcct gcgcctcggc gccgtctacg tgcccatcaa caccgcctac accggctcgg
      781 aagtggaata ctttctgcgc gatgcggagc cctcgctctt cgtctgccgg ccctcggtcc
      841 agccggcgct ggccgaaatt gccggcagcg tgccggttct gacgctcgga gccgatggag
      901 acgggtcgct gctcgagggc gcgctgaaag ctgaagcgcg tgacgaggcg gagccgcgtg
      961 atcctgcaga cctggcggcc attctctaca cctctggcac caccggccgc tccaaaggtg
     1021 cgatgctcag ccagggcaac ctgctgtcca acgccgagag cctgctcgag tgctgggtct
     1081 ggcgcgacga cgacgtgctg ctccacgcgc tgcccatctt tcacgtgcac ggtctgttca
     1141 ttgccctcca ctgcgcgttt ctctccggca cgccggtaac cttccttgcg aagttcgatc
     1201 cggaagccat cgtcgagtgc ctgccggagt ccacggtgct catgggggtc ccgaccttct
     1261 atacgaggtt gctggacttc ccgggtttcg gtgcggacgt ctgccgcaac atgaggctct
     1321 tcatctccgg ctctgcgccg ctcaccgaag cgacctttca cgcgtggcgt gagcggacgg
     1381 gtttcgagat tctcgaacgc tatgggatga gcgaaaccat catcatcgcg tcgaacccgc
     1441 tgaatggcga acggcttgcc ggcacggtgg gttttgccct gccgggcatg gaggtgagga
     1501 tcgccgacga ggagggtaag ccgctgtctg gagacgacgt gggtatgata gaggttcgcg
     1561 gatcgagcgt gttttcgggt tactggcgca tgcccgagaa aacggcggag gagatccgtc
     1621 cggacgggtt tttcgttacc ggggatcttg gccggatgga cgcggacggt cgcgtcacga
     1681 tcgtgggccg atccaaggac ctcgtcatct ccggtggcta caacgtctat cccaaggagg
     1741 tcgagcgtct gatagacgag cttccaggcg tggtcgagtg cgccgtcatc ggcgtgccgc
     1801 atccagactt tggcgaagcc gtggtcgccg tggtcgttcc ggagaacgaa gaggtgagcg
     1861 agcggtcggt agcggcgggg ctggatggcc agctcgctcg attcaagcag cccaaacgcg
     1921 tgatcaacat cggcgagctt ccccgcaacg ccatgggcaa ggtgcagaag aacgagctcc
     1981 gagagcgttt cgccgacctc ttcgcgcaaa gctagaccac gctggtcggc agcaggtcga
     2041 acgtggccgc ctgctccggc ggcagggtaa tggtggcgat g
//
LOCUS       DBNHQR010000266         5571 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1735035, whole genome shotgun sequence.
ACCESSION   DBNHQR010000266 DBNHQR010000000
VERSION     DBNHQR010000266.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5571)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5571)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5571
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1735035"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="AC16VR_06085"
     CDS             <1..181
                     /locus_tag="AC16VR_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008100006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HIR6010762.1"
                     /translation="VINFDLPEDPEDYVHRIGRTGRAGAEGVSISFVSEDDAFNLPSI
                     EEYLGEAVVCTQPSF"
     gene            224..1069
                     /locus_tag="AC16VR_06090"
     CDS             224..1069
                     /locus_tag="AC16VR_06090"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012993.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HIR6010763.1"
                     /translation="MNQGHRLTRVGVVQLCATPDVTANMTAVERLVPQACDAGAEVVL
                     LPEAFAFLGPEKAKRGILERLPDAPSAPGGPILEQCRSMAARLGCHLILGGFHEDAGD
                     PDKSYNTCVHLSPGGELLARYRKVHLFDVALADGTQLRESARTLAGHDLVTTDMPFGK
                     LGLSICYDLRFPYLYQSLVDAGAVAMTVPSAFTKTTGAAHWHVLLRARAIESQSYVIA
                     PAQHGHNWGKRFSYGHSLIVDPWGDIVAECRDGDGVAVADIDPERVRRVREELPSLTH
                     RRLLE"
     gene            1126..2142
                     /locus_tag="AC16VR_06095"
     CDS             1126..2142
                     /locus_tag="AC16VR_06095"
                     /inference="COORDINATES: protein motif:HMM:NF013740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR6010764.1"
                     /translation="MATLIVIAAGIKAAEALMVPFLLAAFIATIAATPVFWLERRRVP
                     AGIAIALVMLGIIVMITGVGAVVAQSAGAFTNRLPFYQDRLTELVENAVTYVEMAGVN
                     ISEEALLSYFDPGTALVMAGNTLRGLGGALSNGFLILLTVIFILAEASSFPRKLQEVL
                     DDPDSDLPHFVRFAENVNRYIGIKTTISIITGVLVGLMLAVLGVDFPALWGLLAFLLN
                     YVPTIGSLIAAIPAVLLALIQLGPLTAAVAGAGFFVINLLMGNVVEPRYMGRGLGLST
                     LVVFVSLVFWGWMLGPVGMLLSVPLTMTAKIAMEANPSSQWLAVLLGPAERAADPTPA
                     EDQT"
     gene            2139..3227
                     /gene="hisC"
                     /locus_tag="AC16VR_06100"
     CDS             2139..3227
                     /gene="hisC"
                     /locus_tag="AC16VR_06100"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 -
                     L-histidinol-phosphate:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HIR6010765.1"
                     /translation="MTHERQNIRAMHGYTWGEQPERADVIKLNTNENPYPPSPAVDRA
                     LADLSGEALRAYPQPTADRLRDQIAELHNLKRENIVVTNGGDEGLRLAFTTFLEPGGT
                     FGMADPSYSLYPVLAAIQDTRVLSIALDENWDLPRDFAHQLNDAGAGLACLVNPHAPS
                     GSLLDTSRISQLANDFNGVLLIDEAYVDFVDPALRYDAVRLTEAFDNLLILRTFSKGY
                     SLAGMRLGYLVGCASLIDPVIGKTRDSYNVDYVSQRVGEAALSDPAYAEETWANVRAG
                     RRTLREGLLEHGFRVGASQTNFLLTEVPAASPCSAKQLYEALKTRGVLVRYFDTPSLR
                     DTLRITVGTPEQNQTLLTWVGDILSRGE"
     gene            3322..4623
                     /locus_tag="AC16VR_06105"
     CDS             3322..4623
                     /locus_tag="AC16VR_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR6010766.1"
                     /translation="MTPKAGSYFPSQAASTLYAMAGDDCSLHFDLNTLTEKQLADHVS
                     NIELTAMLQVALLYVGKPVDHVALFDEFQRPPWRETLDLYRRAFTEMGVPPVPESNCH
                     YYPRDGFYEAVAADRVDADLTTLYLVSGSNAVVHRSDAALSVSQRVNSKMQLAADAAQ
                     WGIPVPETLVCRKRDLDGPEVSGFFDTHHNQIMLKLKGLAGARNVTSVAGVPECMDYV
                     AEYPNDMEIVLQRKLSADEFTEMTVDLIVRDDSIEIANVRRILFADGLWVGNLIGGGV
                     TVDPAHERALLRVGEYVRDLGYRSPEGFNCGIDYFVSADELLVTEINARWTGGLFPAE
                     MIRRLDLAGRGAVAFFDMVSRDGLPRYLDFSEQTLYRRQDEPFASVPLGFSPFPMPVD
                     GRDMHPVWQMVVGDIEAFKAAKREALEPGDLPTADKISLTF"
     gene            4743..5504
                     /locus_tag="AC16VR_06110"
     CDS             4743..5504
                     /locus_tag="AC16VR_06110"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR6010767.1"
                     /translation="MFPLRDENPRVHPPYATVAIIILNVLVWVAVQGFGNHYALAESL
                     CLYGLIPGDLLGNAVPGTSIPLGSNLVCQLDGTANPASLFTSMFMHGGWFHIIGNMWF
                     LWVFGDNVEDVMGPFRFVVFYVVCGLAAAFAQIATNTSSLVPMVGASGAIGGVMGAYA
                     LMFPRAHVHTLIFLGFYVTTVAVPAVFMLGYWFLLQLLQGLPAMGSSQGGVAFWAHIG
                     GFLAGVALIHLFRRPNHLAQHLAQPERRSSRHRWF"
BASE COUNT          931 a         1901 c         1740 g          999 t
ORIGIN      
        1 cgtgatcaac ttcgacctgc ccgaggatcc ggaagattac gtccaccgaa tcggccgaac
       61 cggccgcgcg ggcgccgagg gagtgagcat cagcttcgtc tcggaagacg acgcgttcaa
      121 cctgcccagc atcgaggaat atcttggtga ggccgtggtc tgcacccagc cgagcttctg
      181 agcgggcaac ggtaccgccc aagccgcagg ggacccgggt gccgtgaacc aaggccaccg
      241 tctaaccagg gtaggcgtcg tccagctatg cgcgacgccg gacgtcacgg ccaacatgac
      301 tgccgtcgag cgtctcgtcc cccaggcctg cgatgcgggc gccgaggtcg tgctgctgcc
      361 cgaggccttc gcatttctcg gaccggaaaa ggcaaagcgc ggcatcctgg agcggctccc
      421 cgacgccccg agcgcgccgg gcggtccgat cctggaacag tgccggtcaa tggccgcgcg
      481 cctgggctgc cacctgatac tcggcggctt tcacgaggat gcaggcgatc ccgacaagag
      541 ctacaacacc tgtgttcacc tttctcccgg cggcgagttg ctggctcgat accgaaaagt
      601 gcacctgttc gacgtggcgc tggcagacgg cacccagctg cgcgagtcgg cccgcacgct
      661 ggccggccat gacctggtca ccacggacat gcccttcggc aagctgggct tgagcatctg
      721 ctacgacctc cggttcccct atctttatca gtccctggtg gacgcgggcg ccgttgcgat
      781 gacggttcca tcggcgttca ccaagaccac cggcgccgct cactggcacg tgctgctgcg
      841 ggcgcgggcc atcgagtccc agagctacgt catcgccccg gcacagcacg gccacaactg
      901 gggcaaacgc ttctcctacg gtcactccct gatcgtcgat ccgtggggcg acatcgttgc
      961 cgaatgccgg gacggcgatg gcgtggcggt ggcagacata gaccccgaaa gggtgcggcg
     1021 ggtcagggag gagctgccta gcctcacaca cagacgtctc ctagaatgac ccacaggccc
     1081 tcacggacgg ctgcattggg ttcaacgagc aagacgctca tcgtcgtggc aaccctcatc
     1141 gtcatcgctg caggcatcaa ggcggcagag gcgctgatgg tgcctttcct gctggcggcc
     1201 ttcatcgcca ccatcgcggc tacccccgtt ttctggctcg agcgccgtcg ggtcccggcg
     1261 ggaatcgcca tcgctctcgt gatgctcggc atcatcgtga tgatcaccgg ggtcggcgcg
     1321 gtggtggccc agtcggcggg cgctttcacg aaccggttgc ccttctacca ggaccggctc
     1381 acggagctcg tcgagaacgc ggtgacctac gtggagatgg cgggggtgaa catctccgag
     1441 gaggccctgc tgtcctactt cgatccgggc accgccctgg tcatggccgg caataccctg
     1501 cgcgggctgg gaggcgcgct cagcaacggt ttcctgatcc tgctcaccgt catcttcatc
     1561 ctggccgagg cctcgagctt tccccgcaag cttcaggagg tgctagacga tccggacagc
     1621 gacctgcccc acttcgttcg cttcgcggaa aacgtcaatc gctacatcgg catcaagacc
     1681 accatcagca tcatcaccgg cgttctcgtc gggctgatgc tggccgtgct cggcgtggac
     1741 ttccccgcgc tttggggcct gctcgccttt ctcctgaact acgtgcccac catcggctcg
     1801 ctgatcgcgg cgatccccgc ggtgctgctc gcgctgatcc agctcggccc gctgacggcc
     1861 gcggtggccg gggccgggtt cttcgtcatc aacctgctga tggggaacgt ggtcgagccc
     1921 cgctacatgg gccgggggct gggactttcc acgctggtgg tgttcgtctc cctggtgttc
     1981 tggggctgga tgctcggacc cgtgggtatg ctgctgtcgg tgccgctgac gatgaccgcc
     2041 aagatcgcca tggaagcgaa cccgtcgagc cagtggctgg cggtgctgct cggacctgcc
     2101 gaacgggccg cagatcccac gccggccgag gaccagacat gacccacgaa cgtcaaaaca
     2161 tacgcgccat gcacggctac acctggggcg agcagccgga acgagccgac gtcatcaagc
     2221 tcaacaccaa cgagaacccc tacccgccga gcccggccgt ggaccgggcg ctcgccgatc
     2281 tcagcggcga ggccctgcgc gcctaccccc agcccacggc cgatcgcctg cgcgatcaga
     2341 tcgccgagct gcacaacctg aagcgcgaga acatcgtggt caccaacggc ggcgacgaag
     2401 gcttgaggct ggccttcacc acgttcctgg agccgggcgg caccttcggt atggccgatc
     2461 ccagctactc gctctatccc gttctggcgg ccattcagga cacccgggtc ctgagcatcg
     2521 cgctggacga gaactgggac ctgccgaggg acttcgccca ccagctcaac gacgcggggg
     2581 cgggactcgc ctgcctggtc aatccccatg ccccatccgg ctccctgctc gacacgtcgc
     2641 ggatcagcca gctggccaat gatttcaacg gcgttctgct gatcgacgag gcttatgtcg
     2701 atttcgtcga cccggccctt cgctacgatg cggtgcgcct caccgaggcg ttcgacaatc
     2761 tgctcatcct gcgcacgttc agcaaggggt acagcctggc cggaatgcgt ctcggctacc
     2821 tcgtcggctg cgcctcgctg atcgaccccg tcatcggcaa aacgcgcgac agctacaacg
     2881 ttgactacgt gagccagcgg gtcggcgagg ccgcgctgtc cgatccggcc tatgccgaag
     2941 agacctgggc aaacgtacgc gccggccgcc gcacgcttcg cgagggcctg ctcgaacacg
     3001 gtttccgcgt tggcgcttcg cagacgaatt ttctgctgac cgaggtgccc gcggcgtctc
     3061 cgtgcagcgc aaagcagctc tacgaggcgc tcaagacccg gggagttctg gtgcggtact
     3121 tcgacacccc cagcttgcgg gacaccctgc gcatcaccgt gggcacgccc gagcagaacc
     3181 agaccctgct gacctgggtg ggggacatcc tgagccgcgg agagtaggga ttagtatgca
     3241 cggcttcgca atcaccgctg cgcgcccgct atcgtccgca gcttcggcca acacatcgca
     3301 accccgacgg agagtcagat catgacacca aaggcgggtt cgtatttccc cagccaggcg
     3361 gcatccacgc tctacgccat ggccggcgac gactgctctt tgcacttcga cctgaacacg
     3421 ctgaccgaga agcagctggc cgatcacgta tcgaacatag agctcacggc catgcttcag
     3481 gtggctctgc tctacgtcgg caagcccgtg gatcacgtcg ccttgttcga cgagtttcag
     3541 cgaccgccgt ggcgcgagac gctggacttg tatcgtcgcg cgttcaccga gatgggcgtg
     3601 ccgcccgtgc ccgagtccaa ctgtcactac tatccacggg acgggttcta cgaggcggtg
     3661 gcggcggacc gggtcgacgc ggatctgacc accctctacc tggtgagcgg atccaacgcc
     3721 gtggtgcacc gcagcgacgc cgcgctttcc gtcagccaac gcgtgaactc gaagatgcag
     3781 ctggcggcgg acgctgcgca atggggcata ccggtgcccg aaaccctggt ctgccggaag
     3841 cgcgacctcg acggccccga ggtatccggc tttttcgata cccaccacaa ccagatcatg
     3901 ctgaagctga agggtctggc cggcgcgcgc aacgtcacca gcgtggctgg cgtgccggag
     3961 tgcatggact acgtcgccga gtacccgaac gacatggaga tcgtgctgca acggaagctg
     4021 agcgcggacg agttcaccga gatgaccgtg gacctgatcg tgcgggacga cagcatagag
     4081 atcgccaacg tccggcgcat cctgttcgcc gacgggctct gggtgggaaa tctgatcggc
     4141 ggcggcgtga cggtggaccc cgcccacgag cgcgcgctcc ttcgcgtcgg ggagtacgtg
     4201 agagacctcg gctaccgctc cccggaagga ttcaactgcg ggatcgacta cttcgtcagt
     4261 gccgatgagc tcctggtcac cgagatcaac gcccgctgga cgggcggcct gtttcccgca
     4321 gagatgatcc ggcgtctgga cctggccggt cggggtgccg tcgctttctt cgacatggtc
     4381 tcgcgcgatg gtctgcccag gtatctggat ttttccgagc agactctcta ccgacgccaa
     4441 gacgagccgt tcgccagcgt gccccttggc ttcagcccct ttcccatgcc ggtggacggg
     4501 cgggacatgc acccggtctg gcagatggtc gttggcgata tcgaagcgtt caaggccgcc
     4561 aagcgcgagg cgctcgagcc gggcgacctg cccacggcgg acaagatcag cctgacgttc
     4621 tgagccttca agaccatgcc cggtcttgcg gcggcgccga catcccggca ccctagattt
     4681 tcatgcgccc atctgccgac gcgccgagcc aacaccgtgc ctgaggtcca tacgagccga
     4741 tcatgtttcc actgcgcgac gaaaatcccc gggttcaccc gccgtacgcc accgttgcca
     4801 tcatcattct aaacgtcctc gtctgggtcg ccgtccaggg atttggcaac cactacgcgc
     4861 tggccgagtc cctgtgtctg tacgggctga taccgggcga cctgctgggc aacgccgttc
     4921 ccggcacgag cattccgctg ggcagcaacc tcgtctgcca gcttgacggg accgccaatc
     4981 ctgcctccct cttcacgtcc atgttcatgc atggcggctg gtttcacatc atcgggaaca
     5041 tgtggtttct gtgggttttc ggagacaacg tggaggacgt catgggaccg ttccggttcg
     5101 tcgtcttcta cgtcgtctgc ggcctggccg ccgccttcgc ccagatcgcc acgaacacgt
     5161 ccagcctggt gccgatggtc ggcgcatcgg gcgccatcgg cggcgtcatg ggtgcctacg
     5221 ccctcatgtt cccacgcgcc cacgtgcaca cgctgatctt tctcggcttc tacgtgacca
     5281 ccgtggccgt tcccgcggtt ttcatgctgg gctactggtt tctcctgcag ctgcttcagg
     5341 gtttgccggc aatgggctcg agccaggggg gcgtcgcctt ctgggctcac atcggcgggt
     5401 ttctggccgg ggtggctctg atacacctgt tccgccggcc gaatcacctc gcacagcatc
     5461 tcgcccagcc cgagaggcgc tcctcgcgac accgctggtt ctgagacgcg cggccgccgc
     5521 ggccgaaaac atggcggcaa acgcggttgc gaagcgcacg aggttccgcc a
//
LOCUS       DBNHQR010000267         4453 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_976203, whole genome shotgun sequence.
ACCESSION   DBNHQR010000267 DBNHQR010000000
VERSION     DBNHQR010000267.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_976203"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="AC16VR_06115"
     CDS             complement(<1..378)
                     /locus_tag="AC16VR_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010768.1"
                     /translation="MPQHPREPDIASWHRYFAIECNNAAWDLAGRARTAPEDEQMLQA
                     AHAAAFHWDQVGEELNRMRATMLLAKVHALLGKNPSALRYAEEMQTFFLDRDTPDWEI
                     AFAHAIYAHAAYVAGDYTAHARAY"
     gene            complement(418..2097)
                     /gene="ilvD"
                     /locus_tag="AC16VR_06120"
     CDS             complement(418..2097)
                     /gene="ilvD"
                     /locus_tag="AC16VR_06120"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017338119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIR6010769.1"
                     /translation="MTDQKRPYSSIVVDGVQQAPSRAMLYPVGFTEADFAKPQIGIAS
                     TWSMVTPCNMHIDALAEAAALGADEAGGKSVVFNTITVSDGISMGTPGMRYSLVSREV
                     IADSIETVVGAQGFDGFVAIGGCDKNMPACAMAMARLNRPSVFVYGGTIQPGAERRDI
                     VSVFEAVGAHAAGRISDIELKEVEATAIPGPGSCGGMYTANTMASAIEAMGLSLANSS
                     AQEAISNAKRQDSYQAGAAVFNLIERGIKPSDILCREAFENAITVAIALEGSTNAVLH
                     LLAIAHAAGVPLELDDFSRVGERVPVLADMRPAGQYAMSELIAIGGIQPLMKTLLEEG
                     LLHGDCLTVTGKTLAENLADVAPYPDGQNVIRPMSDPIKKDSHLVVLYGNLAPTGAVA
                     KITGHEGLSFTGSAKCFHGEEAAMAAIMDGAINKGDVVVVRYEGPKGGPGMREMLSPT
                     SAINGRGLSQDVALITDGRFSGGSHGFVIGHITPEAYEGGPIALIEDGDLITVDAEKR
                     EINVQLSDADLAARRARWQKPAPYATKGILAKFARLVSSASEGAVTDKNLD"
     gene            complement(2130..3461)
                     /gene="argA"
                     /locus_tag="AC16VR_06125"
     CDS             complement(2130..3461)
                     /gene="argA"
                     /locus_tag="AC16VR_06125"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF003641.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - L-glutamate N-acetyltransferase
                     activity, acting on acetyl-CoA as donor [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HIR6010770.1"
                     /translation="MSDRPAKAEAENGYARWFRASTPYISAHRNRTFVILLGGDAVAH
                     GNLPNIVHDLALLHVLGARVVVVHGARPQIDAALGESPFHEDRRITDAAAMETIIGIL
                     GQQRARLEALFSTGLPTSPLHNTNISVVGGNFITARPVGIHDGVDHLFTGRPRRVHGG
                     RIQKALDTGALVLLSPLGYSPSGQAFNLAADELAADVAMALDADKLIAFGSDRYVSNE
                     RGEPQPNLTPAQLDTLIDVPALDDTTTRGHLRALLRACRGGVPRCHLVSYAHDGALLE
                     ELFTAGGGGTQISEDHSNLVRQARLEDVAGIVELIRPMEEAGVLVRRPRDRLEQEIDR
                     FFVAEVDGIVMGCCALYPFEDCGELACVAVHESHRSKPGETRLGVALLRATEAAAGAM
                     GLRKLFVLTTQTRDWFVDNGFEDSTLDALPAPKQAMYNYQRNSKVMIKQLR"
     gene            3735..>4453
                     /locus_tag="AC16VR_06130"
     CDS             3735..>4453
                     /locus_tag="AC16VR_06130"
                     /inference="COORDINATES: protein motif:HMM:NF017009.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE/PilB domain-containing protein"
                     /protein_id="HIR6010771.1"
                     /translation="MAAGSEPKVASLASRLPLAQEQLIDLPVLLDGLVKEGYISQRQA
                     EDILITPRTKKELSQHPMEIVADRDFDNRNKPGHKLDLETMTVWLAHQSGQPYLRIDP
                     LDINVGAVTDVMSYAFAQRHNILAVDVTDDEVVIASAQPYMYQWETMLTQTLRGRRIR
                     RVVTNPADLSRYMLEFYTMARSVAKAQDAGMEMSGLANFEQMLELGQMKSPEANDSHI
                     VNIVDWLLQYAFDQRASDIHI"
BASE COUNT          755 a         1432 c         1439 g          827 t
ORIGIN      
        1 ataggctcgc gcgtgggcag tgtaatcccc ggctacgtaa gcagcgtggg cgtagatcgc
       61 gtgggcgaag gcgatctccc aatcgggcgt gtcccggtcc agaaagaagg tctgcatttc
      121 ctcggcgtac cgcagcgcgg aggggttctt gcccagaagc gcgtgcacct tggcgagcag
      181 cattgtggcc cgcatcctgt tgagctcctc gccgacttga tcccagtgaa aggccgcggc
      241 atgcgcggct tgcagcatct gctcatcctc aggcgccgtg cgggcgcggc cggccaggtc
      301 ccaagccgcg ttgttgcact cgatggcaaa gtatcgatgc catgatgcga tgtcgggctc
      361 ccgcggatgt tgcggcacgt ctgcttatcc ccgtttcacg gcatgctgcc gatgggctca
      421 atccaggttc ttgtcggtaa cggcgccttc actcgccgag ctgaccagcc gggcgaactt
      481 cgccagaatg cctttcgtgg cgtacggcgc cggtttctgc cagcgggcgc ggcgcgcggc
      541 caggtccgcg tcgctcaact ggacgttgat ctcacgcttc tcggcatcga cggtgatcag
      601 atcgccgtcc tcgatcagcg cgatgggccc tccctcgtaa gcctcgggcg tgatgtgacc
      661 gatgacgaag ccgtgggagc cgccggagaa acgcccgtcg gtgatcagcg ccacgtcctg
      721 actaagaccg cggccattga ttgcgctggt gggcgagagc atctcgcgca tccccgggcc
      781 gcccttcggc ccttcgtagc gtacgaccac cacgtcgccc ttgttgatgg ccccgtccat
      841 gatggcggcc atggcggcct cctcgccgtg aaagcacttc gcgctgcccg taaaggacaa
      901 cccctcgtgt ccggtgatct tcgccacggc cccggtgggg gccaggttgc cgtagagaac
      961 gaccaggtgg gaatccttct tgatcgggtc gctcatggga cggatgacgt tctggccatc
     1021 cggatacggg gccacgtcag cgaggttctc ggcgagggtc tttccggtga ccgtgaggca
     1081 gtcgccgtgc agcaggccct cttcgagcag cgttttcatg agcggctgga tgccgccgat
     1141 ggcaatgagc tcactcatgg cgtactggcc ggccggacgc atgtcggcca gcacgggaac
     1201 cctctcgccg acgcgggaga aatcgtccag ctccaggggc acgccggctg cgtgggcgat
     1261 ggcgagaagg tgcagaacgg cgttggtcga tccttccagg gcgatggcga cggtgatggc
     1321 gttctcgaac gcctcgcggc agagaatgtc gctgggcttg atcccgcgct cgatcaggtt
     1381 gaacaccgcc gcgccagcct ggtagctgtc ctggcgcttc gcattcgaga tcgcttcctg
     1441 ggctgagctg ttcgccagcg acaaacccat agcctcgatg gcggatgcca tggtgttcgc
     1501 cgtgtacatg ccgccgcagg aaccgggccc cgggatcgcc gtggcctcga cttccttgag
     1561 ctcaatatcg gagatccgtc ccgccgcgtg agcgcccacg gcctcgaata ccgagacgat
     1621 gtcgcgtctt tcggcgccgg gctggatggt cccgccgtag acgaacacgc tcggacggtt
     1681 caggcgggcc atggccatcg cgcaggcggg catgttcttg tcacaaccgc cgatggcgac
     1741 gaacccgtcg aatccctggg cgccgacgac ggtctcgatg gagtcggcga tcacctcccg
     1801 cgaaaccagc gagtaacgca tacccggcgt gcccatgctg atgccgtcgg aaacggtgat
     1861 cgtgttgaac accacgctct ttccgccggc ttcgtcggcg ccgagcgctg ccgcctcggc
     1921 cagggcgtcg atgtgcatgt tgcacggagt gaccatgctc cacgtggagg cgatcccgat
     1981 ctgtggcttt gcgaagtccg cctccgtgaa acccacgggg tagagcatcg cgcggctcgg
     2041 agcctgctgc acgccgtcca cgacgatgga cgagtagggt cttttctggt cagtcatgga
     2101 gttcccctgg ggcccggcct cactgccggt tacctgagtt gcttgatcat caccttcgag
     2161 ttgcgctggt agttatacat agcctgcttg ggcgctggaa gcgcgtcgag agtggaatcc
     2221 tcgaagccgt tgtccacgaa ccagtctcgg gtctgggtgg tcagcacgaa gagcttcctc
     2281 aggcccatcg cgcccgctgc ggcttccgtc gcccttaaga gcgcaacccc gaggcgtgtc
     2341 tctccaggct tgctgcgatg ggattcgtgg acggccacgc acgcgagctc cccgcagtcc
     2401 tcgaagggat acagagcgca gcaacccatc acgatgccgt ccacctcggc aacgaagaaa
     2461 cgatcgatct cctgctccag cctgtcacgc ggacgcctta ccagcacgcc ggcctcctcc
     2521 atgggcctga ttagctcgac gatgccggcc acgtcttcga gccgcgcctg ccgcacgagg
     2581 ttcgagtgat cttcgctgat ctgggtgccg cctccgccgg ccgtgaagag ctcctccagc
     2641 agcgcgccgt catgcgcgta gctcaccagg tggcaacgcg gcactccgcc tcgacaggcg
     2701 cgcagaagcg ctcgcagatg accgcgggtg gtcgtgtcat ccagggccgg aacgtcgatc
     2761 agggtatcga gctgggcggg tgtcaggttg ggttgcggct cgccgcgttc gttgctgacg
     2821 tagcgatcgc tgccaaaggc gatcagcttg tcggcatcca gggccatggc cacgtctgcc
     2881 gcgagctcgt ccgcggccag gttgaacgcc tgcccggaag gcgagtagcc caggggagac
     2941 aacagcacga gcgcgccggt atccagggct ttctggattc gccccccgtg gactcgtcgc
     3001 ggccggccgg tgaacaggtg gtccacgccg tcatggatgc cgacgggtcg cgccgtgatg
     3061 aagttgccac cgaccacgga gatgttcgtg ttgtgcagtg ggctggtggg cagcccggtg
     3121 gagaagaggg cctccagtcg ggcgcgctgc tgtcccagga tgccgatgat cgtctccatg
     3181 gcggctgcgt cggtgatgcg gcgatcctcg tgaaagggcg attccccgag ggccgcatcg
     3241 atctgcggcc gtgcgccgtg caccacgacc acccgtgcac ccaggacgtg cagcagcgcg
     3301 agatcgtgaa cgatgttcgg caggtttcca tgggcgacgg cgtcgccccc gagaaggatg
     3361 acgaacgttc ggttacggtg cgcgctgatg tagggggtcg acgccctgaa ccatcgtgcg
     3421 tatccgttct ctgcttctgc cttggccggt ctgtcgctca tgaattgctg ccctgccgcc
     3481 gcccccctcg ggaagcgcgt cgtcaaaaaa gggggcgcat tttacaccag cgacctcgaa
     3541 tggcatggca aacgtgacgc cggtctaatt ccgaaaatgc catcgccggc tctgggcgct
     3601 agccggttgc aaaagccgca tccttgtcat taactgaaac ttcttggcta atatcaggga
     3661 cttgcgtggc caaccccctc attgattctc cggcgggcag ggatccgcgt ccgctgtcgg
     3721 agggagctgc tccgttggca gccgggtcgg agccgaaggt cgcctccctt gccagccgcc
     3781 tgccgttggc gcaggagcag ctcatcgacc tgccggtgct tctcgacggg ctcgtcaagg
     3841 aaggctacat cagccagcgt caggcggaag atatcctgat cacgccgcgc accaagaaag
     3901 agctcagcca gcatcccatg gagatcgttg cggatcgcga tttcgacaac cgtaacaagc
     3961 ctggccacaa actcgacctc gagaccatga cggtctggct ggctcatcaa agcggtcagc
     4021 cctatctgcg catagatccg ttggacatca atgtcggcgc ggtcacggac gtgatgtcgt
     4081 acgcctttgc gcaacggcac aacatcctcg ccgtggacgt gaccgacgac gaagtcgtca
     4141 tagccagcgc ccagccctac atgtaccagt gggagaccat gctcacccag accctgcggg
     4201 gccggcgcat cagacgggtg gtgactaacc ccgcggactt gagccgctac atgctcgagt
     4261 tctacaccat ggcccgctcg gtggcgaagg ctcaggatgc ggggatggag atgagcggcc
     4321 tggcgaactt cgagcagatg ctcgagctcg gccagatgaa gtctccggaa gccaatgatt
     4381 cgcacatcgt caacatcgtc gactggcttc ttcagtacgc gttcgaccag cgggccagcg
     4441 acattcacat cga
//
LOCUS       DBNHQR010000268         3002 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_911909, whole genome shotgun sequence.
ACCESSION   DBNHQR010000268 DBNHQR010000000
VERSION     DBNHQR010000268.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_911909"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="AC16VR_06135"
     CDS             <1..648
                     /locus_tag="AC16VR_06135"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
                     /protein_id="HIR6010772.1"
                     /translation="PQLTGIAGGALDHREEVRRLCDEFRGHVPGTTLRNGKPNIIDIN
                     PDNDTESIGTFSGSLDDDLWSAKLKLDWRPNDDLLLYASYNRGVKGAGFNAPLDITVG
                     DGVTVIPDLEQETAFKEETLNAYEIGFKATLSNGLARLNGAVYYYDYEDFQAFKIMRQ
                     AGPAGGCAIPQQAPLQPDPRRGEHGERLHGGQCPRRLPIGRRSLGASGPRFQLHR"
     gene            complement(807..2909)
                     /locus_tag="AC16VR_06140"
     CDS             complement(807..2909)
                     /locus_tag="AC16VR_06140"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-containing oxidoreductase family
                     protein"
                     /protein_id="HIR6010773.1"
                     /translation="MTRIEKRTFCRVCEPACGLIATVEDGALTALRGDADHPVSKGFV
                     CNKGIYGLDIHNDPDRLRTPLKRTANGFEPIGWEQALEEIAGKLRRILDNHGTSALAT
                     YTGNPNAFNALYGPSFGNFARQLGVRKNFSSGTQDCSNKFAGSEAVFGTRTMHPLPDI
                     DHSDFILILGENPAVSHMSFCSIPNPMQHLKEAEQRGARILYVNPREIESARMAGSVL
                     RAKPDTDAFLLAAMIHEIYASRGFHDDVSRHGSHAEGLIEFIRQYDPERVAPITGIPA
                     ETIRQLARDFADAPSACAHMSTGVNMGRLGTMAYWLLHMLVLVTGNLGRRGGNFYSLG
                     FYERSPSAGRNVPEGHLETPFGAVRKPGGVGISLPGNLLASYLADPDEPVRAMFVNAG
                     NPVLSIAGEAATRQALENLELLVCVDIYRNATGEYAHYVLPAAGAFEREDINITGLGM
                     QFQPSVQFTEAVVPPAFERKPDWWIYEKLSQAMGFASAFDSGEPDMWSRIDAMLRSQG
                     RSMMQLRAEQIIALERSRPEEFYERYVQTEDGRIDCMPETFADGVDRMAAIFGELEEE
                     PANQLKLISKRDRYMLNSWYSNVAKLKSGERDRNYLFMHPEDAAIRQIADGDRVRISN
                     DNGEITSQARLTAELMPGVVAMTHGWGHGRTPGMRVAQEKPGTNCNALLPSGPGSFDP
                     LSNQAHMTGVPVEVAPAG"
BASE COUNT          517 a          938 c          992 g          555 t
ORIGIN      
        1 cctcagctca cgggcatcgc cgggggtgcg ctggatcatc gagaggaagt ccgaagatta
       61 tgcgatgaat ttcgtggcca cgtgccggga accaccctcc gcaatggcaa accgaatatc
      121 atcgacatca acccggacaa cgacacggag tccatcggca cgttctcggg ctcgctcgat
      181 gacgacctct ggtcggccaa gctgaagctt gactggcggc ccaacgacga tctgctcctg
      241 tacgccagct acaaccgtgg ggtgaaaggc gccgggttca acgcgcccct ggacatcacc
      301 gtcggtgatg gtgtgactgt gatccctgac ctcgagcaag agacggcgtt caaggaagag
      361 accctgaacg cctacgagat cggattcaag gcgaccttga gcaacgggct ggcccggctg
      421 aatggcgccg tctactacta cgactacgaa gacttccagg ccttcaagat catgcggcaa
      481 gctggccctg caggcggatg cgcaataccg cagcaagcac cacttcagcc tgacccgcgc
      541 cgaggcgagc acggagaacg gctacacggt ggccaatgcc cgcgtcgcct accgatcggg
      601 cgacgatcgc tgggagctag cggcccacgt ttccaacttc accgatgagg aatacctggt
      661 gcagaccttc gatctcgccg gcgtgctcgg catgacggag cagtactacg gcatgcccgg
      721 gtggtggtgg gtaacggctt cgtaccagtt cggaaactga ccgggctgtc cggcaacggc
      781 agccccgcgc gctgagaggt cgccgctcac ccggcgggcg ctacctccac gggcacgccc
      841 gtcatgtggg cctggttcga cagtgggtcg aagctgccgg ggcctgacgg taacagggca
      901 ttgcagttgg tgcccggttt ctcctgagcg acacgcatgc cgggggtgcg gccgtgaccc
      961 cagccgtggg tcatggccac cacgccgggc atgagctcgg cagtcaatct ggcctggctc
     1021 gtaatctcgc cgttgtcgtt gctgattcgg acccgatccc cgtccgcgat ctggcggatg
     1081 gcagcgtcct cgggatgcat gaagaggtag ttccgatccc gctcgcccga cttgagcttg
     1141 gccacgttgc tgtaccagct gttgagcatg taccggtccc ttttgctgat cagcttcagc
     1201 tggttggccg gttcctcctc gagctcgccg aagatggcag ccatacggtc gacgccatcg
     1261 gcaaacgttt cgggcatgca gtcgatccga ccgtcctccg tctgcacgta gcgctcgtaa
     1321 aactcttcag gcctcgaccg ctcgagagcg atgatctgct cggcgcgcag ctgcatcatg
     1381 ctgcgtccct gggaccgcag catggcgtcg atccgtgacc acatgtcggg ctcgccgctg
     1441 tcgaacgcgg aagcgaaccc catggcctgg ctcagcttct cgtagatcca ccagtccggt
     1501 ttgcgctcga aggccggtgg gacgacggcc tcggtgaact gcacgctggg ctgaaactgc
     1561 atccccagcc ccgtgatgtt gatgtcttca cgctcgaagg ctccggcggc gggcagcacg
     1621 tagtgggcgt actcccccgt cgcgttccgg taaatgtcca cgcagacgag cagctccagg
     1681 ttctccagcg cctgtcgcgt ggcggcctca ccggcaatgg acaacaccgg attacccgcg
     1741 ttgacgaaca tggcgcgtac gggctcgtcg ggatccgcca gatagctggc gagcagattg
     1801 cccggcaggc tgatgcccac ccctcccggt ttgcgtaccg ccccgaaagg cgtttccaga
     1861 tggccctcgg ggacgttccg gccggccgaa gggctgcgct cgtagaatcc cagagagtag
     1921 aagttgccgc cgcggcgacc gagattgccg gtaacgagca cgagcatgtg cagcagccag
     1981 taggccatgg tgccgagccg gcccatgttg acccccgtgg acatgtgggc gcaggcgctc
     2041 ggggcgtcgg cgaagtcgcg tgcgagctgg cggatggtct cggcgggaat gccggtgatc
     2101 ggagcgaccc gctcgggatc atattgccgg atgaactcga taaggccttc ggcatggctg
     2161 ccgtgacggg acacatcatc gtgaaacccg cgtgatgcgt atatctcgtg aatcatcgcg
     2221 gccagcagga acgcgtcggt gtcgggcttt gccctgagca ccgagccggc catgcgggcg
     2281 gactcgattt cccgggggtt cacatacagt atccgggcgc cgcgctgctc ggcctccttc
     2341 aggtgctgca tggggttggg aatggagcag aaggacatgt ggctgacggc cggattctcg
     2401 ccgaggatca ggatgaagtc ggagtggtcg atgtcgggca gcgggtgcat ggttcgggtg
     2461 ccgaagacgg cctcgctgcc cgcgaacttg ttgctgcagt cctgggtgcc ggagctgaag
     2521 ttcttccgca cgcccagctg ccgcgcgaag tttccgaacg agggcccgta cagcgcattg
     2581 aaggcgttcg ggtttcctgt gtacgtcgcc agggccgatg ttccgtggtt gtccaggatg
     2641 cggcgcagct tcccggcgat ctcctcgaga gcctgctccc agccgatggg ctcgaagccg
     2701 ttggcggtgc gtttgagcgg agtgcgcaag cggtccggat cgttgtggat gtccagccca
     2761 tagatgccct tgttgcagac gaagcccttc gagacagggt ggtccgcgtc gccgcgcagc
     2821 gcggtcagcg caccgtcctc gacggtggcg atcaggccgc aggcgggctc gcagactctg
     2881 cagaacgtgc gtttctcgat gcgtgtcacc gtaaccctcc gaattgctga tccgatcata
     2941 gctcagcgcc gcttgctgcg tgaggtcttg ccggtgcggc gggacccgtc gagaatgtgg
     3001 ct
//
LOCUS       DBNHQR010000269         4966 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1293245, whole genome shotgun sequence.
ACCESSION   DBNHQR010000269 DBNHQR010000000
VERSION     DBNHQR010000269.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4966)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4966)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4966
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1293245"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="AC16VR_06145"
     CDS             complement(<1..862)
                     /locus_tag="AC16VR_06145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HIR6010774.1"
                     /translation="MRKFTLMPFLLPLLLLCLASSGCSWMPWVGKDKDKAEEDLNATE
                     QQLYRDTQRALRAGNYDRAIQGLQQLEARFPFGRYAEQAQLEIIYAQYMSYDHDTARA
                     SADRFMRLHPQHANIDYAYYIKALAAFNKNRGLLDRIFATDLAKRDMTAARDAYADFA
                     QLLALYPDSQYAPDANLRMIYLRNLLAESEIHVADYYMRRGAYLAASNRARYVIETYS
                     KSQSVDDALAILVEANWRLGLEEAANDALRVLALNYPNYPAFDTDGNLTLASTVRNRD
                     RSWTNMVTFGL"
     gene            950..1918
                     /gene="rluD"
                     /locus_tag="AC16VR_06150"
     CDS             950..1918
                     /gene="rluD"
                     /locus_tag="AC16VR_06150"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HIR6010775.1"
                     /translation="MAEPLEIQKRVTVPPDVAGERVDKAAARLFPEFSRAALTAWIQD
                     GALTVDGARVKPKVKLVGGETLVLDAVVAPREDWSTGEPVPFDVLYEDADLLVVNKPA
                     GLVVHPGAGNPDGTLVNGLLHHRASLALVPRAGIVHRLDKDTSGLLLGAANLEAHATL
                     VRALQARQIQRGYRAVCEGVLVSGLDIDAPMGRDPVRRTLQRVRADGRPALTRVRVVE
                     RFRAHTLVVAQLETGRTHQIRVHLSSIGHPLVGDRAYGARGRLPPGAQDELIAALRGF
                     SRQALHAAKLAFDHPVHGGRLQFEAPDPDDLVDLLDALRQDVRHHG"
     gene            1911..2663
                     /gene="pgeF"
                     /locus_tag="AC16VR_06155"
     CDS             1911..2663
                     /gene="pgeF"
                     /locus_tag="AC16VR_06155"
                     /inference="COORDINATES: protein motif:HMM:NF014619.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HIR6010776.1"
                     /translation="MVEANAWEWARWPDDAPGHRIAAVTTTRPGGFSKGKYAAFNLAC
                     HVHDDPAHVARNRRDLLKALGLAHVQWLEQVHGTGCVQATLASCDRVPVADAAWTSVP
                     GLALAVLTADCLPVVVAARDASTVAVIHAGWRGLVAGIIPSVVGSLPGRTTGYVGWIG
                     PGIGADAYEVGGDVAEAVRQSGPEAEAYLRPGGRADKYQLDLAGLAVRQLGDVGIGCV
                     ERSSACTWSDARFYSYRRDGETGRQATLVWIR"
     gene            complement(2702..3178)
                     /locus_tag="AC16VR_06160"
     CDS             complement(2702..3178)
                     /locus_tag="AC16VR_06160"
                     /inference="COORDINATES: protein motif:HMM:NF024901.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HIR6010777.1"
                     /translation="MRKFISLAFAALCIPVLATADVYRWVGPDGVVNYTQMKPEGVQA
                     ERVQAQGGRRSTAAPAPSEPADQSATQSADTSQLSPKQQEMLNDLQSAEAARKQEVAR
                     IRESNCERSRRVLDRMSATARIRVRDDSGEERIMGEDERQNRIAEAQQGVAENCLS"
     gene            3510..>4966
                     /gene="ilvB"
                     /locus_tag="AC16VR_06165"
     CDS             3510..>4966
                     /gene="ilvB"
                     /locus_tag="AC16VR_06165"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019342702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HIR6010778.1"
                     /translation="MGEELSGGDILIRALQDAGVDYIFGYPGGSALHIYDAIFRQQKV
                     EHILVRHEQAATHMADAYARATGKPGVVLVTSGPGATNAITGIATAHMDSIPMIVISG
                     QVPNPLIGTDAFQETDMVGISRPVVKHSFLVKDCSEIASVVKKAFHIATTGRPGPVVI
                     DVPKDTTDPDITAPYAFPQSVSLRSYSPAVRGDGRQIRKAVDVLLEAKRPIIYSGGGV
                     IQGDGSELLTRIARRLGFPVTNTLMGLGGFPGDDEQFLGMLGMHGTYEANMAMHNADL
                     ILAIGARFDDRVTNNPSKFSPAATVVQVDVDPASISKIIPADIPVVGQVASVLEEMWA
                     ILESELERRPHLPDAEALKAWWGEIDGWRQAHGLDHSLQPEPGKPILPQLVVQTLYRV
                     TQGEAYVTSDVGQHQMFAAQYYKFSQPRRWINSGGLGTMGFGLPAAMGVQFAFPDARV
                     VCVTGEGSFMMNMQELSTCLQYGLPIKIVNLNNQAL"
BASE COUNT          854 a         1515 c         1690 g          907 t
ORIGIN      
        1 gcagaccgaa cgtgaccatg ttggtccagg atcggtcgcg gttgcgcacg gtgctcgcca
       61 gggtcaggtt gccgtcggtg tcgaacgccg ggtagttcgg gtagttgagc gccaataccc
      121 gcagtgcgtc gttggcggcc tcctccagcc ccaggcgcca gttggcctca accagtatgg
      181 ccagcgcatc gtcgacggat tgggacttgg agtaggtctc gatgacgtat ctcgcccggt
      241 tgctggccgc cagataagcg cccctgcgca tgtagtagtc ggccacgtga atctccgact
      301 ctgccaggag gttccgcaga tagatcatcc gcaggttcgc gtcgggcgcg tactggctgt
      361 cggggtaaag ggccagcagc tgagcgaagt cggcgtaagc gtcgcgggcg gcggtcatgt
      421 cccgttttgc aagatcggtc gcgaagatgc gatccagcag gccccggttc ttgttgaagg
      481 cggccagagc cttgatgtag taggcgtagt cgatgttggc atgctgagga tggaggcgca
      541 tgaaccggtc cgcggacgcg cgggccgtgt cgtgatcgta ggacatgtac tgagcgtaga
      601 tgatctccag ctgggcctgc tccgcgtagc ggccgaaagg aaaccgggcc tcgagctgct
      661 ggagaccctg gatcgcccgg tcgtagttgc cggcccggag cgcgcgttgc gtgtcgcggt
      721 agagctgctg ctccgtcgcg ttcaggtcct cttcggcttt gtccttgtcc ttgccgaccc
      781 agggcatcca ggagcagccg gaagacgcaa ggcaaagcag taggagaggc aaaagaaagg
      841 gcatgagggt gaatttgcgc atggatcctg cagctttggt tcaggcgtga gccagaaagt
      901 ttcgctaatt taacgattta gcgtacggtt gcaaccagag cagagggcga tggcggagcc
      961 gctggaaatc cagaaacgag taacggtgcc acctgacgtg gcgggagagc gcgtcgacaa
     1021 ggcggcggcc cggctgttcc cggaattctc ccgcgccgcg ttgaccgcct ggattcagga
     1081 tggggcgctc acggtggacg gtgcccgggt caaaccaaaa gtgaagctcg tagggggcga
     1141 aacactcgtg ctcgatgccg tcgtcgcgcc ccgggaagac tggagcactg gggagcccgt
     1201 cccgtttgac gtcctctatg aagacgccga cctgctcgtc gtcaacaagc ctgcggggct
     1261 agtggttcat cctggagcgg gcaatcccga tggcacgctc gtcaacgggc tgctccatca
     1321 ccgggcctcg ctggccctgg ttccgcgggc ggggatcgtg caccgtctgg acaaggacac
     1381 gtccgggctg ctcctgggtg cggccaatct cgaagctcac gccactcttg tcagggcgct
     1441 gcaggccaga cagattcagc ggggctatcg cgccgtctgt gaaggggtgc tggtcagcgg
     1501 gctggatatc gatgccccga tggggcgcga tcccgtgcgc aggacgctcc agcgcgttcg
     1561 ggccgatggt cgccccgcgc tcacccgggt ccgggtggtc gagcgtttca gggctcacac
     1621 cctggtggtc gcgcagctgg aaaccggacg cacccaccag atcagagtgc atctgagcag
     1681 tatcgggcat ccgctggtgg gggaccgagc ctacggggct cggggacgtc tgccaccggg
     1741 tgctcaggac gaactgatcg ccgcgctccg cggtttctcg cgccaggcgc tgcatgccgc
     1801 gaagctcgcc ttcgaccacc ccgttcacgg cgggaggttg cagttcgaag ctccggaccc
     1861 cgacgatctg gtggatttgc tcgatgccct gcggcaggac gtgcggcacc atggttgaag
     1921 cgaacgcttg ggagtgggcc aggtggccgg acgacgcgcc cggccaccga atcgccgcgg
     1981 taaccacgac ccggccgggc gggttcagca aggggaagta tgcagccttc aatctcgcct
     2041 gccacgtcca cgatgacccg gcccatgtgg cccgaaaccg tcgcgatctg ctcaaagcgc
     2101 tcggtcttgc gcacgtgcaa tggctcgagc aggtccatgg aacaggctgt gtccaggcca
     2161 ccctggcaag ctgcgaccgg gtgccggtgg ccgatgccgc gtggacgtct gttcccgggc
     2221 ttgcgctggc cgtccttacc gccgattgcc tgccggtggt cgttgctgct cgcgatgcga
     2281 gcacggttgc cgtgatccac gcgggttggc gcggcctggt tgccggcatc atccccagcg
     2341 tggtgggctc gctacctggc aggacgacgg gatacgtggg ctggattggt ccgggaatcg
     2401 gagccgacgc ttatgaggtt ggcggagacg tggccgaggc ggtgcggcaa tcgggccccg
     2461 aggcggaagc ctacctgaga ccgggcggac gggcggacaa atatcagctt gacctggccg
     2521 ggctggccgt tcggcagctt ggggacgtgg ggatcggatg tgtcgagcga tcctcggcat
     2581 gcacctggtc cgacgccagg ttttattcgt atcggcgcga tggcgagacc ggtcgccagg
     2641 caacgctggt ctggatacgg tgagctggca gtgggcccgc acggctgcgg gccccgggtc
     2701 ctcaggacaa gcagttctct gcaacgccct gctgggcttc agcgatgcga ttctggcgct
     2761 catcctcgcc catgattctt tcctcgccgg aatcgtcacg aacgcgtatg cgggcggtgg
     2821 cggacatgcg gtcgagcacc cgtcgtgacc gctcgcagtt ggattcccgg atccgggcga
     2881 cttcctgctt gcgggcggcc tcagccgact gcagatcgtt cagcatttcc tgctgcttag
     2941 gggaaagctg tgacgtgtcc gcgctctggg tggcggactg gtcggcgggc tcgcttgggg
     3001 ctggggcagc agtcgaacgt cttccgccct gggcctggac gcgctcggcc tggacaccct
     3061 caggtttcat ctgagtgtag ttgacgaccc cgtcgggacc gacccagcgg tagacgtcgg
     3121 cggtcgccag taccggtatg cagagagcag cgaaggcgag actaatgaac ttgcgcatca
     3181 tccagatact cctgatatat gccacggccg ccggcggttt ttttcgtgac cggcgcccgg
     3241 gcgtagcaat ccaatccggg attttagagc gaccggggcg tccgtccctt gcggcagtta
     3301 ccccggatat gtgaagcgat tcgcaatttg gcgcattgga ccgttcgttg acatgccgtg
     3361 ggcgaacccc aagaatacgc ggctccggcg gtccctcctt tcaaaccccg tgaaaaatca
     3421 aacgtttatg ggtggcggga catcagccgg tgaggtatct ggttgactaa cgggcacgaa
     3481 tcacagtggg cacagacaag agtcagagca ttggcgaaga gctctccggc ggcgacatcc
     3541 tgatacgggc gctgcaggac gccggcgtgg attacatctt cgggtacccg ggcggctccg
     3601 ccctgcacat ctatgacgcc atattccgcc agcagaaggt ggagcacatc ctggtgcgtc
     3661 acgagcaggc cgcgacgcac atggcggatg cctacgcgcg cgccacgggg aagcctggcg
     3721 tcgtgctggt gacctcgggc ccgggcgcta ccaacgccat caccggaatc gccacggccc
     3781 acatggattc cattcccatg atcgtgatct cgggacaggt ccccaacccg ctgatcggaa
     3841 cggatgcgtt tcaggaaacg gacatggtcg gtatctcgcg gccggtggtg aagcattcgt
     3901 tcctggtcaa ggattgcagc gagatcgctt cggtggtcaa gaaggcgttc cacatcgcca
     3961 ccaccgggcg tcccggtccg gtggtcatcg acgttccaaa ggacaccacg gaccccgaca
     4021 tcaccgcgcc ttacgccttc ccgcagtcgg tgagcttgcg ttcctacagt ccggcggtca
     4081 ggggtgacgg gcggcagatc cgcaaggccg tggacgtgct gctggaagcc aagcgaccga
     4141 tcatctactc gggtggaggc gtgatacagg gcgacgggtc ggaactgctc acgcgcattg
     4201 cccggcgcct gggcttcccg gtgaccaata cgctgatggg tctgggcgga tttcccggcg
     4261 acgacgagca gttcctcggc atgctcggca tgcacgggac ctacgaagca aacatggcca
     4321 tgcacaacgc ggatctgatc ctggccatcg gcgcgcgctt tgacgaccgt gtcaccaaca
     4381 acccgagcaa gttctcgccc gcagccaccg tggtgcaagt cgatgtggat ccggcttcca
     4441 tctccaagat catcccggcg gacatacccg tcgtcgggca ggtggcgagc gtgctcgaag
     4501 agatgtgggc aatcctggaa agcgagctcg agcggcgtcc gcatctgccg gacgccgaag
     4561 cgctcaaggc atggtggggc gagatcgacg gatggcggca agcgcacggg ctcgatcact
     4621 ctcttcagcc ggagccgggc aagccgatcc tcccgcagct ggtggtgcag actctctatc
     4681 gcgtcaccca gggcgaagcc tacgtgacca gcgatgtggg ccagcaccag atgtttgcgg
     4741 cccagtacta caagttctct cagccgcggc gctggatcaa ctccggcggc ctcggcacca
     4801 tgggcttcgg gctgcccgct gccatgggcg tgcagtttgc ctttccggat gcgcgggtgg
     4861 tctgcgtcac cggcgaggga agcttcatga tgaacatgca ggagctgtcc acctgccttc
     4921 agtacggtct gccgatcaag atcgtcaacc tcaacaacca ggccct
//
LOCUS       DBNHQR010000270         2531 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_468397, whole genome shotgun sequence.
ACCESSION   DBNHQR010000270 DBNHQR010000000
VERSION     DBNHQR010000270.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_468397"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1864
                     /locus_tag="AC16VR_06170"
     CDS             <1..1864
                     /locus_tag="AC16VR_06170"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6010779.1"
                     /translation="HATENLRERLTMAARQSINLWQLTRGQRLRYAGAIFAMGMTNLF
                     IFGPVLVSGSAIDVIADQDLSHAVPPLLWAASRLSEGAPFAAYLWVAAVVAVTMTAIG
                     GAFLFARGRFAAVASEAIVRNLRERLYDRLHDLEAGFFDTADTGDLVQRASSDVETLR
                     VFLASDVVEIGRAIMLIMCVAPILAWLDVRLAALSLCLMPLLAIGAYLFFARVKDVFQ
                     ITDESEAAMTATLQENLTGIRVVRAFARQAFEIEKFGARNAEFRNNNRRLIQLMGIYW
                     STSDLFAMTQIGIVLIAGGLFVLDGSITVGTLFEFMTMVSMVIWPVRQLGRVLTDTGK
                     AVVSLGRINEVLTEAEERAGTIPGEGRARGEIRIENLTFGYDPQRPVLEDVNLHVEVG
                     ETLAIVGPPGSGKSSLIRVLLRMYPYQKGSVRIDGWEVAELDRLWLRHQIGVVLQDPF
                     LYSRSIRANLALGRPGAPEKELIEACHDAAVHDAIIGFPEGFEAMVGERGVTLSGGQR
                     QRMALARALLKDPPILVLDDSLSAVDTGTERRILDAMRRRKGRQTTLIIAHRLSSVMH
                     ADRILVLQEGRVVQLGNHATLAGEPGPYRTLCEIQGALDASIEADTNATGRGNG"
     gene            1857..>2531
                     /locus_tag="AC16VR_06175"
     CDS             1857..>2531
                     /locus_tag="AC16VR_06175"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HIR6010780.1"
                     /translation="MDNYDDENFDDGFDDEALAARVDLKLWRKLFAYARRYPKDLVWL
                     AVCAFTTASMEVTYPLITKWVVDDVDAAYAAGTVISLWPHALAYLACTVTICIAIGGF
                     IWMGGKIRTHVSHDIRRDGFENLQRLSFAFYDYRPVGWLMARMTSDCERLSNILAWGF
                     LDLVWGSTMMLGIAVAMLVMNPKLALVVLAILPLLGWVSARFQRRILSSARRVRATNS
                     RITASFN"
BASE COUNT          417 a          837 c          813 g          464 t
ORIGIN      
        1 ccatgcaacc gagaatctgc gggagcgatt gacgatggca gccagacaat cgatcaacct
       61 atggcagctg accagaggtc agcggctccg ctatgccggg gccatcttcg ccatggggat
      121 gaccaacctg ttcatattcg gccccgtgct ggtgagcggc tcggcgatcg acgtcatcgc
      181 cgatcaggac ctcagccacg ccgtcccgcc tctgctctgg gcggccagca ggctttccga
      241 gggcgcgccc ttcgccgcct acctctgggt ggccgccgtg gtcgcggtca ccatgacggc
      301 catcggcggc gccttcctgt tcgcgcgcgg ccggtttgca gcggtggcgt cggaagccat
      361 cgtccgcaac cttcgcgagc ggctgtacga ccggctgcac gatctggaag ccggcttttt
      421 cgacacggca gacacgggcg atctcgttca gcgcgcgagc tcggacgtgg aaacgctgcg
      481 cgtcttcctg gcaagcgacg tggtcgagat cggtcgggcc atcatgctca tcatgtgcgt
      541 cgcccccatc ctcgcgtggc tggacgtccg gcttgccgcg ctgtctctgt gcctgatgcc
      601 cctgctggcc atcggagcct acctcttctt cgcccgggtg aaagacgtgt tccagataac
      661 ggacgagtcc gaagccgcca tgaccgccac gctccaggag aacctcaccg gcatccgcgt
      721 ggtacgggcg ttcgcgcggc aggcgttcga gatcgagaaa ttcggcgcgc ggaacgcaga
      781 gttcaggaac aacaatcgcc ggctgatcca gctgatgggc atctactgga gcacgagcga
      841 tctgttcgcc atgacccaga tcggcatcgt gctcattgcg ggggggctgt tcgtgctcga
      901 cggttccatc accgtcggca cccttttcga gttcatgacc atggtctcca tggtgatctg
      961 gcccgtccgc cagctcggcc gggtgcttac ggatacgggc aaggcggtgg tgtcgctggg
     1021 caggatcaat gaggtgctca cggaagccga ggagcgggcc ggcaccatcc cgggcgaggg
     1081 gcgggcacgc ggggagattc gcatcgagaa cctgacgttc ggctacgacc cgcagcgtcc
     1141 ggtgctcgag gatgtgaacc tgcacgtgga ggtcggcgag acgctggcca tcgtcggccc
     1201 cccgggatcc ggcaagtcca gcctgatccg cgtgcttctg cgcatgtatc cgtaccagaa
     1261 gggatccgtg cggatcgatg ggtgggaggt cgccgagctc gaccggctct ggctgcgcca
     1321 ccagatcgga gtggtcctgc aggacccctt tctctactcg cgcagcattc gcgccaacct
     1381 tgcgctgggg cggcccggcg cgcccgagaa agagctcatc gaggcatgcc acgacgcggc
     1441 cgttcacgac gccatcatcg gcttcccaga aggtttcgaa gccatggtcg gcgagcgcgg
     1501 cgtcacgctt tccggcgggc agcggcagcg catggccctg gcacgcgccc tgctgaagga
     1561 cccgcccata ttggttctcg acgattccct ttccgcagtg gatacgggaa ctgagcgcag
     1621 gatcctggat gccatgcgcc gccgcaaggg ccgccagacg accctgatca ttgcccaccg
     1681 actgtcgtcg gtgatgcacg cggaccgcat tctcgttctg caggaaggac gtgtggttca
     1741 gctcggcaat cacgccacgc tggccgggga gcccggaccg taccggacgc tctgcgagat
     1801 ccagggtgcg ctggatgcat ccatcgaggc ggacaccaac gccacaggac gcggcaatgg
     1861 ataactacga cgacgagaac ttcgacgacg gcttcgacga cgaagcgctg gcggccaggg
     1921 tcgacctgaa gctgtggcgc aagctgttcg cctacgcccg acgctacccg aaggacctcg
     1981 tgtggctggc cgtctgcgct ttcaccaccg cctcgatgga ggtcacgtat cccctgatta
     2041 ccaagtgggt ggtagacgac gtcgacgccg cttacgcagc cggcacggtg atctccctgt
     2101 ggcctcatgc gctcgcctac ctcgcgtgca cggtgaccat ctgcatcgcc atcggcgggt
     2161 tcatctggat ggggggaaaa atccgcaccc acgtgagcca cgacatacgt cgcgacggct
     2221 tcgagaacct gcagcggctg tccttcgcct tctacgacta ccgtccggtt ggctggctca
     2281 tggcgcgcat gacgtcggac tgcgaacggc tctccaacat cctggcctgg ggattcctcg
     2341 acctggtctg gggatcaacc atgatgctgg gcatcgccgt ggccatgctt gtcatgaacc
     2401 cgaagctggc cctcgtggtg ctcgctatcc tgccgcttct cggctgggtc tcggcccgtt
     2461 ttcagcgccg gattctgtcc agcgcccgcc gcgtacgcgc gacgaattca cgcatcacgg
     2521 cttccttcaa c
//
LOCUS       DBNHQR010000271         3068 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_595922, whole genome shotgun sequence.
ACCESSION   DBNHQR010000271 DBNHQR010000000
VERSION     DBNHQR010000271.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_595922"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="AC16VR_06180"
     CDS             <1..428
                     /locus_tag="AC16VR_06180"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012794.7"
                     /note="threonine deaminase; threonine dehydratase; in
                     Escherichia coli, IlvA is part of the isoleucine
                     biosynthetic pathway; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HIR6010781.1"
                     /translation="ATDREAQVYVGLQVSAADDRAELMKSLSTGYVVHDMTDNEAAKL
                     HVRHMVGGRTFGSKPELLYRFEFPERPGALLRFLTGLGSRWNITMFHYRNHGAAWGRV
                     LVGFEAQPRDRRSLGEYLKRIGYRYWEETDNPAYQLFLR"
     gene            complement(435..968)
                     /gene="def"
                     /locus_tag="AC16VR_06185"
     CDS             complement(435..968)
                     /gene="def"
                     /locus_tag="AC16VR_06185"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR6010782.1"
                     /translation="MAVRKIIRMGHPTLRRVARPLAGEEIRSEAVGRLIGDMIDTLHD
                     YGGIGLAAPQINESVRLAIIEIPGGPTRYGEIPALPLTVFINPSVEVVDPATAGYWEG
                     CLSVPGLRGFVERPQHVRVNALDQEGKPLDLELKGFLATVFQHEFDHLDGRLYVDHLK
                     DPALLAFDEEFERYLAE"
     gene            complement(988..1572)
                     /locus_tag="AC16VR_06190"
     CDS             complement(988..1572)
                     /locus_tag="AC16VR_06190"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HIR6010783.1"
                     /translation="MTSTLERQELRRFFRERRRALGSHQQREHALRVARHLLNNPVSW
                     RSRRIGAYWANDGEVSLAPLLGELEYRNRQLALPVVSTAGRMAFYRYHRAAPMLVNRY
                     GIPEPGPGSAFVDGRSLDLILLPLVAFDEYGVRLGMGAGYYDRFLGALPEPLRPRIVG
                     IAHEVQRSADPLPFQDWDIPLDGIVTERGWQAFT"
     gene            complement(1594..1804)
                     /gene="ssrS"
                     /locus_tag="AC16VR_06195"
     ncRNA           complement(1594..1804)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="AC16VR_06195"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(1877..2191)
                     /locus_tag="AC16VR_06200"
     CDS             complement(1877..2191)
                     /locus_tag="AC16VR_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017521389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0000921 - septin ring assembly [Evidence
                     IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HIR6010784.1"
                     /translation="MSEEATTVSVKILDKEYQVSCPAEEVDALSRSARYLDEQMRSIR
                     ESGKIFGMDRIAVMAALNIANEFINSSKSLESTHGVVSQKVTELTERVGRALSEQKQL
                     NL"
     gene            complement(2205..2411)
                     /locus_tag="AC16VR_06205"
     CDS             complement(2205..2411)
                     /locus_tag="AC16VR_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02449 family protein"
                     /protein_id="HIR6010785.1"
                     /translation="MADTEWTALESKVDELIELCSVLTKENRALRAQQQNWTTERAKL
                     IEKNELAKARVESMITRLKALEQD"
     gene            2533..>3068
                     /locus_tag="AC16VR_06210"
     CDS             2533..>3068
                     /locus_tag="AC16VR_06210"
                     /inference="COORDINATES: protein motif:HMM:NF015642.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HIR6010786.1"
                     /translation="MADLTALALGAVESLSVAEIHGAVCGLAVASEGGIELGSLVELV
                     GIEALRDEHSVGEFVNAAVDALHAEDLSFAPLLPDDHVDLALRLEALGEWCGAFLAGF
                     ASGSRFQELGEEQIPEEAREIIGDFTYIANVDGSEAGDGEDPESAERDYAEVHEYVKV
                     GALLLRTLLGGDAPEETAR"
BASE COUNT          534 a          936 c         1001 g          597 t
ORIGIN      
        1 tcgccacgga ccgggaagca caggtctacg tcggcctgca ggtatcggcg gccgacgata
       61 gggccgagct catgaaaagc ctctccacag gctacgtcgt gcacgacatg acggacaacg
      121 aggctgcaaa gctgcacgtc cgacacatgg tgggcgggcg caccttcggc agcaagccgg
      181 agctcctcta tcgattcgag tttcccgagc ggccgggtgc gctgcttcgc tttctgaccg
      241 gcctggggtc tcgctggaac atcacgatgt ttcattatcg taatcatggg gccgcatggg
      301 ggcgcgttct ggtgggcttc gaggctcagc cacgcgaccg gcgctccctg ggcgagtacc
      361 tcaagcgcat cggctaccgc tactgggaag agacggacaa cccggcctac cagctgtttc
      421 tgcgctaggg gccgttactc ggccagatag cgctcgaact cctcgtcgaa tgccagcagg
      481 gctggatcct tgagatggtc cacgtacaac cgaccgtcca gatggtcgaa ctcgtgctga
      541 aacaccgtgg cgaggaaccc tttcagctcc agatcgagcg gtttgccctc ctggtcgagc
      601 gcgttcacgc gaacatgctg gggccgttcc acgaatcctc gcagccccgg aaccgacagg
      661 cacccctccc agtagcccgc ggtggcagga tccacgacct ccacggacgg gttgatgaac
      721 accgtcagcg gcagagcggg gatctcgccg tagcgggtgg ggccgccggg aatctcgatg
      781 atggccagcc gcacggactc gttgatctgc ggggccgcga gacctatgcc gccgtagtcg
      841 tgaagggtgt cgatcatgtc gccgatcaga cggcccaccg cctcgctgcg gatctcttcg
      901 ccggccagcg gcctggcgac ccttcggagg gtggggtgtc ccatgcggat gattttgcga
      961 acggccatgg gcggagacga tacggggtca ggtgaacgct tgccagccgc gctcggtcac
     1021 gatgccgtcc agagggatgt cccagtcctg gaacggcagt gggtcggcgc ttcgctgcac
     1081 ttcgtgggcg atgccgacga tccggggccg aagcggttcg ggcagcgcgc ccaggaaccg
     1141 gtcgtagtag cccgcaccca tgcccaggcg aacaccgtac tcgtcgaagg cgaccagcgg
     1201 gagaaggatc aggtccaggc ttctgccgtc cacgaaagcc gagccggggc ccggctcggg
     1261 aatcccgtag cggttcacca gcatgggcgc ggccctatgg taacggtaaa aggccattct
     1321 ccctgcggtg ctgactaccg gcagagcgag ctggcggttt cggtactcca gctcgccgag
     1381 caggggcgcg aggctcactt ctccatcgtt ggcccagtac gcgccgattc gcctgctgcg
     1441 ccatgacacg gggttgttga gcaggtgtcg ggctacccgg agcgcgtgct ctcgctgctg
     1501 atgagagccg agggcccgcc tgcgctcacg gaagaaacgg cgaagttcct gacgttcgag
     1561 ggtgctggtc atggcaggga accgggttgg aaagagtctc ccgaaaatgc cgctgtttgg
     1621 tttgccctga accgttgtgg ttcaaggcgg ggacccgggc gacgtctaag gcttcccgat
     1681 ctgcaccacc gtttggataa ccgcgggcgg tatcggacag gctcaccggc gtcagcgccg
     1741 aggtctccta tagtttatct aacggctcaa ggacgacacc aactggcgaa cattccagga
     1801 gacagcacgg agtatagccc aattgacgtc gttgccacgg gttgacaacc ggtcacgatc
     1861 gagaactgtg aaaccgttaa aggttcagct gtttctgttc cgagagcgcg cggccgaccc
     1921 gctcggtgag ctcggtcacc ttctggctga cgacgccgtg agtggattcc aaagacttgg
     1981 agctgttgat gaactcgttg gcgatgttca gcgcggccat cacggcgatc cggtccatcc
     2041 cgaaaatctt gccggactcg cgtatggaac gcatctgctc gtcaaggtag cgggcagacc
     2101 tcgagagcgc gtctacctct tcggcgggac agctgacctg atactccttg tcgaggatct
     2161 tcacgcttac ggtggtggct tcttcgctca tgtcccctgc ttcgctaatc ctgctcgagc
     2221 gccttcagtc gggtgatcat cgattcgacc cgggctttgg ccagctcgtt tttctcaatg
     2281 agcttcgccc gctccgtggt ccagttctgc tgttgtgctc gcagcgcccg attctccttg
     2341 gtcagcacgc tgcatagctc tatgagctca tccactttgc tctcgagcgc cgtccattct
     2401 gtgtcggcca tgccaggctt cacgcgtatt ctgcttatcg caggatagtg tcgcctgaat
     2461 gggctggcat gtgaagcatg taacttctgc tagcattagg cctatcccgg ggcacgaagc
     2521 tcgataggcg acatggcgga cctgacggcg ctcgcacttg gtgcggtgga atctctttcg
     2581 gttgccgaga tccacggcgc cgtgtgcggg ctggcagtcg cctccgaggg gggaatcgag
     2641 ctgggctctc tcgtcgagct ggtgggcatc gaggcccttc gggacgaaca ctccgttgga
     2701 gaatttgtta acgccgccgt ggacgcgctc cacgccgagg atctcagctt tgctccgctc
     2761 ctcccggacg accatgtgga cctggcgctg cgcctggaag cgctgggcga atggtgcggc
     2821 gcgttcctgg ccggctttgc ttcaggatcc cgatttcagg aactcggaga agagcagatt
     2881 ccggaggagg cccgggaaat catcggcgac ttcacctata tcgccaacgt tgacggctcg
     2941 gaggccggcg acggggagga tccggaatcc gcggagaggg actatgccga ggtgcacgag
     3001 tacgtgaagg tcggcgcgtt gcttctgcgc accctcctgg ggggcgacgc gcctgaagag
     3061 acggcgcg
//
LOCUS       DBNHQR010000272         4025 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1609448, whole genome shotgun sequence.
ACCESSION   DBNHQR010000272 DBNHQR010000000
VERSION     DBNHQR010000272.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4025)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1609448"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="AC16VR_06215"
     CDS             <1..294
                     /locus_tag="AC16VR_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016489939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HIR6010787.1"
                     /translation="VRRADVVKVHSLRRGAAEAIEAIAHGDAKNSKVVGRAIGDVDLP
                     TGASIGAVVRGKEVFVGRDDVVVEPDDHVILFLADKRQVAAVERLFQVGLTFF"
     gene            318..1739
                     /locus_tag="AC16VR_06220"
     CDS             318..1739
                     /locus_tag="AC16VR_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HIR6010788.1"
                     /translation="MTGTLLMLFSIALLLPVVVAVYYGESTTRTFALAFAITLISGLA
                     LTLVARSRRELRSGDGFLITVLFYLGLGLFGAIPIYLAESVDASFTDAAFESLSGLTT
                     TGATVLTGLDELPRSILFYRQLLQWLGGMGIIVLAVAILPMLGIGGMQLYRAESPGPV
                     KDAKLTPRITETAKALWYLYVGITASCALAYYAAGMDAFDALCHSFSTVAIGGFSTHD
                     ASIGHFDSAAVEGVAIFFMILAGINFGLHFMAWNRRNPFLYTRDAEVRLYLIVLVGVA
                     ALVCAILASYPDATESPWRDGLFQAVSIATTTGFTTQDFSVWPSAAPVLLMFAAFAGG
                     CAGSTAGGVKMIRVLLIYRQGIREIRRLIHPQGVFPIKLGRHRVPDRVVDAVWGFFSF
                     YVIIFLAMVACLMLISDLDFLTAFSAVGACLNNLGPGLGEVALNYAALPVSVKWLLIF
                     AMLLGRLEIFTLLVLMTPAFWRR"
     gene            complement(1749..2624)
                     /locus_tag="AC16VR_06225"
     CDS             complement(1749..2624)
                     /locus_tag="AC16VR_06225"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015251.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR6010789.1"
                     /translation="MKGWLILALLRATSILPLAAARALARAAAAAALTLDVRAARTTR
                     INVALCFPELGPEEQARLVRDSLAHSASVVAEAGPLYHWPRARWQTRLTLVGDELFNE
                     AMASGRGVLLLGPHFGNWEALPLFLGRYAVTGLYDPPRISALEKTVRAARERNGMRLL
                     PIDARGLRGVYQALSAGSAAGILPDQVPGRNAGVYAPFFGHPALTMTLAHRLIRRTRP
                     VVLLVAARRVSGGFRIEFHAAPPDILAPDPETSAAAMNSAIESLVRTDPSQYQWEYKR
                     FRRQPAGVADPYRVG"
     gene            complement(2621..3205)
                     /locus_tag="AC16VR_06230"
     CDS             complement(2621..3205)
                     /locus_tag="AC16VR_06230"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007160143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HIR6010790.1"
                     /translation="MVKARRCGWCGDDPLYVAYHDREWGMPERDPTVLFERLVLEGMQ
                     AGLSWLTILNKRENMRERFFAFDPARLAAAGEAEVAAWLNDPGIIRHRGKLEAMISNA
                     RAYLRQGDFSNLLWSAVGGAPRQNRWRHLGDVPSQTGESRDLARMLKSAGFGFVGPTT
                     CYAFMQSAGLVNDHLVSCPAQQRCADAWTALCGG"
     gene            3257..3796
                     /gene="gmhB"
                     /locus_tag="AC16VR_06235"
     CDS             3257..3796
                     /gene="gmhB"
                     /locus_tag="AC16VR_06235"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016852304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HIR6010791.1"
                     /translation="MARLILLDRDGVINFDSPDYIKSPDEWRAIPGSIEAIAALRSGG
                     RIVAVCTNQRGIARGKFSLSDLEAIHAKMSKALADAGTRVDAVTFCPHAADAGCNCRK
                     PKPGMLLSMMERFQVSPEETVFVGDTIRDLEAAAAAGCAAVLVRSGKGPEEEAEALRR
                     GLSFTAYDDLAQFTRAMGP"
     gene            3796..>4025
                     /locus_tag="AC16VR_06240"
     CDS             3796..>4025
                     /locus_tag="AC16VR_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR6010792.1"
                     /translation="MAGLRSLVFFAGYAVITIAWGALSLLIAWLMPYRARFLFIVGAW
                     TRMILAWLRLTCGIRCKVEGRENIPEQPCIVLA"
BASE COUNT          637 a         1332 c         1308 g          748 t
ORIGIN      
        1 gtgcgacggg ccgacgtggt caaggtgcac agcctgcgcc gcggtgctgc agaagccatc
       61 gaggccatcg cccacggcga cgcgaaaaac tccaaggtgg ttggccgggc gatcggggat
      121 gtcgacctgc cgaccggcgc gtccatcggc gccgtggtcc ggggcaagga agttttcgtc
      181 ggccgtgacg acgtggtggt ggagcccgac gaccacgtga tcctgtttct ggcggacaag
      241 cgtcaggtgg cggcggtgga acggctgttc caggtcgggc tgaccttttt ctgatgcatt
      301 tctccgtcat tctccgagtg accggaacgc tgctgatgct gttcagcatt gcgctcctgc
      361 tgcccgtggt ggtggccgtc tactacgggg agagcaccac ccgcacgttt gccctggctt
      421 tcgccatcac cctgatttcg ggcctggcgc tgaccctggt cgcacggtcc cggcgcgagt
      481 tgcgaagcgg cgacgggttt ctcatcaccg tgctcttcta cctgggtctc ggcctgttcg
      541 gggcaatccc catctacctg gccgagagcg tcgatgcgtc tttcaccgac gccgctttcg
      601 agtcgctgtc ggggctgacg accaccgggg cgacggtcct gaccgggctc gacgagctcc
      661 cccgttccat ccttttctac cggcagctgc ttcagtggct cggcggtatg gggatcatcg
      721 ttctcgcggt ggcgatcctt cccatgctgg gcatcggcgg catgcagctg tatcgcgccg
      781 agtccccggg gccggtcaag gatgccaagc taacgccgcg catcaccgag acggcgaagg
      841 ccctgtggta tctctacgtc ggcatcacgg catcctgcgc gctcgcttac tacgccgccg
      901 ggatggacgc cttcgacgcc ctttgccaca gcttttccac cgtcgccatc ggcggcttct
      961 cgacccacga cgccagcatc ggccacttcg acagcgcggc cgtggaagga gtggccatct
     1021 tcttcatgat tctcgccggg atcaatttcg gcctgcactt catggcatgg aaccgacgca
     1081 acccgtttct gtacacgcgt gacgcggagg ttcgtctcta cctgatcgtt ctggtagggg
     1141 tcgcggcgct ggtctgcgcc attctcgcaa gctatccgga cgccacggag tccccgtggc
     1201 gggacggcct ctttcaggcc gtatccatcg cgacgaccac cggttttacc acccaggact
     1261 tcagcgtctg gccctcggcc gcgcccgtcc tgctgatgtt cgccgcgttc gccggagggt
     1321 gcgccggctc gacggcgggc ggggtcaaaa tgatccgcgt gttgctcatc taccgccagg
     1381 gcattcgcga gatccgccgg ctcatacacc cccagggcgt attccccatc aagctgggcc
     1441 gacatcgcgt ccccgaccgt gtcgtcgacg ccgtctgggg gttcttttcg ttttacgtga
     1501 tcatctttct ggccatggtc gcctgcctga tgctgatctc cgatctggat ttcctcaccg
     1561 ccttctcggc ggtgggcgcc tgcctgaata acctggggcc cggcctcggc gaggtggcgc
     1621 tcaactacgc cgcgctcccc gtatcggtga agtggttgct gatcttcgcg atgctgctgg
     1681 gccgactgga gatcttcacg ctgctcgttc tgatgacccc agccttctgg cgccgctgaa
     1741 gcgcccggtt acccgacgcg atagggatcc gcaactcctg cgggttgccg cctgaatcgc
     1801 ttgtactccc actggtactg ggacgggtcg gtgcggacga gcgactcgat ggccgagttc
     1861 atggcagccg ctgaagtctc gggatccggc gcgaggatgt ccgggggcgc ggcgtgaaat
     1921 tcgatccgga agccgccgct tacccgtctg gccgcgacaa gcagcacgac gggccgggtc
     1981 cgccggatga gccggtgagc cagggtcatg gtcagcgcgg ggtgaccaaa gaagggggcg
     2041 tacacacccg cgttccggcc cggaacctgg tcgggaagaa tgcccgcggc ggagccggcg
     2101 ctgagcgcct gatacacccc gcgcagccca cgggcatcga tgggcagcaa tcgcatgccg
     2161 tttctttccc tggcagccct cacggttttt tccagagcgg agattcgggg cgggtcgtag
     2221 agcccggtga ccgcgtagcg gccgagaaac agaggcagcg cctcccagtt gccgaaatgg
     2281 ggaccgagca gcagaacgcc tcgaccggac gccattgctt cgttgaatag ctcatctccc
     2341 acgagcgtca agcgggtttg ccaacgcgcc cggggccaat ggtaaagcgg tccggcctcc
     2401 gcgacgacgc ttgccgagtg cgcgaggcta tcgcgcacca gccgagcctg ctcttcggga
     2461 ccaagctccg gaaagcaaag cgcgacgttg attcgggtcg tgcgggcggc gcggacgtcg
     2521 agtgtcaggg cggccgcggc agcggccctt gccagcgccc gggccgccgc caacggcagg
     2581 atgctcgttg cccgcagcag ggcaaggatg agccagccct tcaaccgccg caaagggccg
     2641 tccaggcatc tgcgcacctt tgctgcgcgg ggcagctgac caggtggtcg ttgaccagcc
     2701 ccgcactctg catgaacgcg tagcaggtgg tgggccccac gaagccgaag ccggcggact
     2761 tcagcatccg ggcaagatcc cgggactccc cggtctggct gggtacatcg cccagatgcc
     2821 gccaccgatt ctgccgcggc gcgccgccaa ccgcagacca cagcagattg gagaagtctc
     2881 cctgccgcag ataggcccgc gcgttggaga tcatcgcctc gagcttgccg cgatgccgga
     2941 tgatacccgg gtcgttcagc cacgccgcca cctcggcctc gccggctgct gcgaggcgag
     3001 cagggtcgaa tgcgaagaag cgctcgcgca tgttttcgcg tttgttgaga atcgtgagcc
     3061 acgaaaggcc tgcctgcatc ccctcgagca ccagccgctc gaaaaggacc gtgggatccc
     3121 gttccggcat gccccactcg cggtcgtggt aggcaacgta gagcgggtcg tcgccgcacc
     3181 agccgcatcg gcgtgctttg accatgcctt tcagatccct tagattgcgc cggcgatttt
     3241 acccggctgc tcagcgatgg ccagactcat cctgctggac cgcgacggcg tgatcaactt
     3301 cgattcgccc gactacatca agtcacccga cgaatggcgg gctatccccg gatccatcga
     3361 agccatcgct gcgctgagaa gcggcggccg catcgtggcc gtgtgcacca accagcgagg
     3421 gatcgctcgc ggcaagttct cgctgagcga tctggaagcc atccacgcga agatgagcaa
     3481 agcgctcgcc gacgctggaa cccgcgtgga tgcggtgaca ttctgccccc atgccgcaga
     3541 cgccgggtgc aattgccgca agccaaagcc gggtatgctg ctctccatga tggagcgatt
     3601 ccaggtatcc ccggaagaga cggtgttcgt gggcgacacc atccgcgacc tggaggctgc
     3661 cgctgccgcc gggtgcgcgg cggttctggt ccggtccgga aaagggccgg aggaggaagc
     3721 cgaagccctt cggcgcgggc tcagcttcac cgcgtacgac gatcttgccc aatttacgcg
     3781 agccatgggg ccatgatggc gggactgaga tctctcgtgt tcttcgccgg ttacgcggtc
     3841 atcacgatcg cgtggggggc gctttcgctc ctgatcgcct ggctcatgcc ctatcgcgcc
     3901 cgatttctgt tcatcgtcgg ggcgtggacg cgcatgatac tggcctggct caggctgacc
     3961 tgcggcatcc gctgcaaggt tgaaggccgg gagaacatac cggagcaacc ctgcatcgtg
     4021 ctggc
//
LOCUS       DBNHQR010000273         3760 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_279073, whole genome shotgun sequence.
ACCESSION   DBNHQR010000273 DBNHQR010000000
VERSION     DBNHQR010000273.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_279073"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="AC16VR_06245"
     CDS             complement(<1..950)
                     /locus_tag="AC16VR_06245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6010793.1"
                     /translation="MRTTYLTAGAIALAIVVWLLSGQMASGDPVRHQTLAENNAEALA
                     RTQDKAPTRVRARVMNATPQFQHVVLRGKTESKRVVEVRSETSGRVIERPVERGAYVT
                     QGDLLCRLAMDDREAALAEARESLNQARIEYEGSLKLKQRGFQSDTAIAQTKARLAAA
                     EAKLARSALEVSRTEIRAPFDGVVETIHQDVGDYVNVSAPCVTVVDPDPMLLIGRVAE
                     KDVHRLLLDQNATGLLSNGTTVTGPVTFIGQQSDPATRTYAVEIQIPNPERAIRSGIT
                     TEIRIPVAETMAQKVSPAVFTLDDAGHIGIRIVNADYRVEL"
     gene            complement(1154..3370)
                     /locus_tag="AC16VR_06250"
     CDS             complement(1154..3370)
                     /locus_tag="AC16VR_06250"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR6010794.1"
                     /translation="MVAWRLGPAAFAALVWGLTGATSSSLAAAADQAAGAQTLAPPRV
                     VLVLSGGGARAAGHIGVLKALEELRVPVHGIVGTSMGAVVGGLYAAGHSPAGMERLVA
                     RVDWQELLIDDPPRESFRFRRKREDASLFVQAASGVDTEGLKLPKGVVHGHRLKAFLA
                     RHTLHVGDVDDFRQLSIPFASIATDISTGEAVELTGGNLAEAMFASLAIPAFVGPTEI
                     DGRLLVDGGVSNNLPVDVALAMGADVIIAVDLSSPLRGRDELGSVLEVTDQLTSILTR
                     RNTTEQIARLRPQDVLIVPELGGVTAIDFDLAATTIPSGYSAAIAARPRLASLALPEE
                     PFEQHVAGLRRQEVTTIDVAEVVLTTDSRVDADLLLARTGLQPGTMEVAQIEAAVDEL
                     YGMELFSQVPYAYQHGELHINPIRRDWGPNYLQFGLALEDNLEGRNHYNLGVAFTATE
                     LNAKAGEWRTELGIGERPRLVTEFFQPFGPRGRWYVNPSVGVSAFNRGVFETDNLVAE
                     YQIRQVAGTLATGYAFGSRAEARIGLSLAEGTRRRLVGDPTFEKTSADSGMAFVEFRY
                     DSLDDLYFPQRGSTLQARWTLSEESLGAEVDYRAAELAASRALSFGRHTLVVGGEFAT
                     VYEGEAPFHEQYNLGGFLNLSGLEVDQKLGQHRGIGRVVYYYRWSSNPVLPVYLGMSA
                     EAGQVWIEDDEIGFDNLEAAGSLFLGLDSPIGPVFLAGGMAEGGNKSIYVFLGQPF"
     gene            complement(3364..>3760)
                     /locus_tag="AC16VR_06255"
     CDS             complement(3364..>3760)
                     /locus_tag="AC16VR_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018405431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6010795.1"
                     /translation="RVTDLDDSLDFYCAKLGLHEVGRHESEAGRFTLVFLAAPGDEEA
                     QVELTYNWDPQEYTGGRNFGHLAYQVEDIYALCQHLMDSGVTINRPPRDGNMAFIRSP
                     DGISVELLQKGGALAPADPWKDMPNVGEW"
BASE COUNT          632 a         1197 c         1268 g          663 t
ORIGIN      
        1 agttcgacgc ggtagtcggc gttgacgatg cgaatgccga tgtgccccgc atcgtcgagc
       61 gtgaagacgg cagggctgac tttctgggcc atggtctcgg ccacggggat gcgaatctcg
      121 gtggtgattc cggagcggat cgcccgctcc ggattgggaa tctggatctc caccgcgtag
      181 gtgcgggtag cgggatcgct ctgctggccg atgaaggtga cggggccggt gacggtcgtc
      241 ccgttactca acagcccggt cgcgttctgg tccagaagga gccggtgcac gtctttctcg
      301 gcgacccgcc cgatcaaaag catggggtcg ggatcgacga cggtgacgca gggcgcgctc
      361 acgttgacgt agtcgcccac gtcctggtgt atggtttcca ccaccccgtc gaagggcgcg
      421 cggatctccg tgcgggagac ctccagagcg cttcgggcga gcttggcttc ggcggctgct
      481 agacgcgcct tggtctgggc gatggcggta tcggactgaa agccccgctg cttgagcttc
      541 aggctccctt catactcgat ccgggcctga ttgagggatt cgcgggcctc ggccagtgca
      601 gcctcgcgat cgtccatggc aagccggcac aggagatccc cctgggtaac gtacgcaccg
      661 cgctcgactg gtctctcgat cacgcggccg gacgtctcgg agcggacctc gacgacgcgc
      721 tttgattcgg ttttccccct caagacgacg tgctgaaact gcggcgtggc gttcattacc
      781 cgggcgcgca ccctggtcgg tgccttgtcc tgggtccgcg cgagggcttc cgcgttgttc
      841 tctgccagcg tctgatgcct gacagggtcg ccgctcgcca tctggcccga aagcagccag
      901 acaacgatgg ccagcgcgat ggcgcctgcg gtgagatatg tggttcgcac tttggtctcc
      961 gatgagcaag tggtgttact aaagcaccca atacgttacc taaagtaaca aactgccggt
     1021 ggtatttcca tgggccgctg cacaatcaag gatgctcggc gggcctggaa gctgtcaggg
     1081 ttaaaggtag cgatggcggt gtgtaggcgg cgtgaaaacg ccgcgcgatg tggctctaaa
     1141 ggcggccttg cggctaaaac ggttgcccga ggaagacgta gatggacttg ttcccccctt
     1201 cggccatgcc cccggcgaga aaaacgggcc ctatgggact gtccagacca agaaacaggc
     1261 tgccggccgc ttccaggttg tcgaagccga tctcgtcgtc ctcgatccag acctggccgg
     1321 cttctgccga catgccaaga tagacgggca gaacggggtt cgaagaccag cggtagtagt
     1381 acacgaccct tccaatgccc cggtgctgac cgagcttctg gtcgacctcc aggccggaga
     1441 gattgaggaa tccgcccaga ttgtactgct cgtgaaaggg ggcttcgcct tcgtagaccg
     1501 tggcgaattc cccgccgacc accagggtgt ggcgcccgaa gctcagcgct cgagaagcgg
     1561 caagctccgc ggcacggtag tcgacctccg cccccagaga ttcctccgaa agcgtccacc
     1621 tggcctgcag ggtgcttcca cgctgcggaa agtacagatc atccaggctg tcgtagcgaa
     1681 actcgacgaa ggccattccc gagtccgcgg acgtcttctc gaaggtaggg tcgccgacga
     1741 ggcgccgccg cgtaccctcg gccaggctga gcccgatgcg ggcctcggcc cggctgccga
     1801 atgcgtaccc cgtggccagg gtgcctgcga cctgccggat ctggtactcg gcgaccaggt
     1861 tgtccgtctc gaacacgccg cgattgaagg cgcttacgcc cactgagggg ttcacgtacc
     1921 agcgtccccg ggggccgaag ggctggaaga actcggtaac caggcgcgga cgctcgccga
     1981 ttccgagctc cgttcgccac tcgccggcct ttgcgttgag ctccgtggcg gtgaaggcga
     2041 cgcccagatt gtaatgattg cgtccttcca ggttgtcctc gagcgcgagt ccgaactgca
     2101 gatagttcgg accccagtcc cgcctgatgg ggttgatgtg gagctccccg tgctgatacg
     2161 cgtagggcac ctgggagaaa agctccatgc cgtagagctc gtccacggcg gcttcgatct
     2221 gcgcgacctc catggtgccg ggctgcagtc ccgtccgggc cagcagaaga tcggcgtcca
     2281 cccggctgtc ggtggtcagc accacctccg cgacgtctat ggtggtgacc tcttgccggc
     2341 gcagaccggc gacgtgctgc tcgaagggct cttccggcaa cgccagtgat gccaggcgag
     2401 gccgggcggc gatggccgcg ctgtagccgg acgggatggt ggttgctgcc aggtcgaaat
     2461 cgatggcggt caccccgccg agctcgggca cgatgagcac gtcctgtggg cgcaaccggg
     2521 caatctgctc cgtggtgttg cggcgggtga gaatgctggt cagctggtcg gtcacctcca
     2581 ggacggagcc gagctcgtcg cggccgcgca ggggagagct cagatccacg gcgatgatca
     2641 cgtcggcacc catggccagg gccacgtcca ccggcaggtt gttggaaacg ccgccgtcca
     2701 cgaggagacg cccatcgatc tccgtggggc cgacgaaagc gggtatggcg aggctggcga
     2761 acatcgcttc ggcgagattg cccccggtga gctccacggc ctccccggtg ctgatgtccg
     2821 tggcgatgct ggcgaacggt atgctcagct ggcggaagtc gtcgacgtcc ccgacgtgca
     2881 gggtgtgccg ggcgagaaac gccttcaggc ggtggccgtg aaccacgccc ttgggaagct
     2941 tcagaccttc cgtatcgacg ccggacgcgg cctggacgaa aagactcgca tcttcgcgct
     3001 tgcgtcggaa acggaaggat tcccggggcg gatcgtcgat gagcagttcc tgccaatcca
     3061 cacgggcaac gagccgttcc attcccgccg gcgagtgacc ggccgcgtag aggccgccga
     3121 ccacggcgcc catgctggtg ccgacgatgc cgtgaacggg gacccgcaac tcctcgagcg
     3181 ctttgagcac gccgatgtgc ccggcggccc gggcgccgcc gccgctgagc accagcacca
     3241 ccctaggtgg ggcgagcgtc tgcgctcctg cggcctggtc ggcggcagca gccagggatg
     3301 atgaggttgc gcccgtgagg ccccagacca gcgcagcaaa ggccgcgggc ccgaggcgcc
     3361 acgctaccat tcgccgacgt tcggcatgtc cttccaggga tcggcgggcg ccagagcccc
     3421 gcccttctgc agcagctcta ccgagatgcc atcgggtgac cggatgaaag ccatgttccc
     3481 gtcacgtggc gggcggttga tggtgacgcc cgagtccatc aggtgctggc acagcgcgta
     3541 gatgtcttcg acctggtagg cgaggtgccc gaagttacgg ccgcccgtgt actcctgcgg
     3601 gtcccagttg taggtcagct ccacctgtgc ttcctcgtcg cctggagcgg ccaggaagac
     3661 gagcgtgaaa cgccccgcct cgctttcgtg ccgaccgacc tcgtgaaggc cgagcttcgc
     3721 gcagtaaaag tcgagtgagt cgtccaggtc agtcacccgg
//
LOCUS       DBNHQR010000274         5105 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_342423, whole genome shotgun sequence.
ACCESSION   DBNHQR010000274 DBNHQR010000000
VERSION     DBNHQR010000274.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5105)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5105)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5105
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_342423"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..382
                     /locus_tag="AC16VR_06260"
     CDS             83..382
                     /locus_tag="AC16VR_06260"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR6010796.1"
                     /translation="MKLDAASACLESLGNSTRLAIYRALVRAGPAGLPVGKLQARLDI
                     PGSTLSHHIAHLVKNDLVSQAREGRVLRCTANYRTMNDLITYLSDECCRDTPRTT"
     gene            352..1044
                     /locus_tag="AC16VR_06265"
     CDS             352..1044
                     /locus_tag="AC16VR_06265"
                     /inference="COORDINATES: protein motif:HMM:NF012455.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HIR6010797.1"
                     /translation="MLSGHPEDHLMARRLAGEFMGTLLLLATVVGSGIMGEQLSGGNA
                     AIALLGNTIATGAILVVIILMFAPVSGAHFNPAVTLTFLLRREIPAGAAAAYCVTQVA
                     AGLAGVWLAHGMFGLEIVQTSENSRSGFGQWLAEGVATFGLVATILAVLRSRPDLVAF
                     AVGLYISAGYWFTASTSFANPAVTIARAFTDTFSGIQPDHVPAFIAAQLVGAALATWT
                     FGWLLSEGERTN"
     gene            complement(1041..1691)
                     /locus_tag="AC16VR_06270"
     CDS             complement(1041..1691)
                     /locus_tag="AC16VR_06270"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF036807.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family protein"
                     /protein_id="HIR6010798.1"
                     /translation="MTLKVIGAGFGRTGTLSLKLALERLGLEKCYHMLEVMQNEGHAA
                     VWRAAARGESVGWDGLFAGYQASVDWPSCNFWEAQLQHFADAKVILTRRDPERWYASV
                     MNTIYPSSVARREQADPAIREHMDMVFEVIWDGLFDGRMDDKEHVIAIYEAHGQHVID
                     ALPPEKLLVFEASAGWGPLCDFLDLPVPDEPYPWVNSTEDFRSRVAAASSKPQRDG"
     gene            complement(1714..2151)
                     /locus_tag="AC16VR_06275"
     CDS             complement(1714..2151)
                     /locus_tag="AC16VR_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014576844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4399 domain-containing protein"
                     /protein_id="HIR6010799.1"
                     /translation="MNGLRSIALALALVTSAAVLADGIRSPAPEGASLYFIAPADGAV
                     VSGPFTVKFGLKGMGVAPAGVDAPNTGHHHLLINLEEAVNPEVPLPATDQVRHFGGGQ
                     TETQLSLPAGTYRLQLMLGNYLHVPHDPPVMSELITVTVADQP"
     gene            complement(2173..2670)
                     /locus_tag="AC16VR_06280"
     CDS             complement(2173..2670)
                     /locus_tag="AC16VR_06280"
                     /inference="COORDINATES: protein motif:HMM:NF016001.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HIR6010800.1"
                     /translation="MTIRDAVWRGFSLFVSLKPVTVFFMNLATWVDRPLMRATSGRIR
                     LSFVIPTLLLRCTGARSGLLREVPLLYVPEGDGALLVGSNGGKPYDPAWCHNLRAQRR
                     VSCLLAGREGTFRVEELSGADRDAAWESAVAIYPGYRRYEQRAGRVIPLFRLIPEADG
                     RARQT"
     gene            complement(2667..3671)
                     /locus_tag="AC16VR_06285"
     CDS             complement(2667..3671)
                     /locus_tag="AC16VR_06285"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6010801.1"
                     /translation="MARDQASWLNQVVEDVLEPDLPICDPHHHLWDHPGSRYLLDELL
                     ADTGSGHNVVATVFVECASMYRADGPEELRSLGETEFVNGVAAMSASGDYGPARVCAG
                     IVGYVDLTLGSSAGPVLDAHMAASDRFKGIRHAAGWDPSEKVRNSHTNPSEGLLLDDE
                     FRAGFAELGKRGLSFDAWLYHPQIPQLTDLARAFPHTTIIFDHFGGPLGIGPYEGRQD
                     QIFAAWQRDVAALAECHNVYAKLGGLVMPVNGFGFHKRERPATSDEIVAATGRYYRHA
                     IDCFGVDRCMFESNFPVDKQSCSYHVLWNAFKKMVADGSPAEKASLFHDTAARAYRLT
                     "
     gene            3771..>5105
                     /locus_tag="AC16VR_06290"
     CDS             3771..>5105
                     /locus_tag="AC16VR_06290"
                     /inference="COORDINATES: protein motif:HMM:NF013109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIR6010802.1"
                     /translation="MRFELRVKNWRSYIPILDVITHYRRSDFSRDLTSGLVVGVITIP
                     QAIAYAFLAGLPAQAGLYACLVPMVIYAILGSSRHLVVGPVAVAALMVAAAVGEHAPL
                     YNESYLGITAVLGLQAGIFLWLLRLWKMGGVVNLLSHPVIVGFVNAAALLIIVSQLNA
                     FTGIADEGSKNPIDRLGQLVSKLGELHTTTTIIGVCCVVGLWLVRRWGVSVAELLVHP
                     LGFSLGDRQLIARLGPLLVASVAVIVVWAGDLNGTAGVATIGLVPGGLPSLTVPPFNW
                     DMWVDLAPSSAMIAVVAYVESFSIGTMIATRQRTRINAHQELIALGAANIGAAFTGAY
                     PVAGSFSRSSVNYQTGARTPVSSLVCAAVIILTLLFFTPLFTYLPHAALAAIVIVSVT
                     SLLDFRSFRTHWRIHREDSVTQMVTLASVLLFGVEAGLLTGVTLSIAFFIRQS"
BASE COUNT          865 a         1705 c         1603 g          932 t
ORIGIN      
        1 cgcgccaata ccgtttcgtt atttctagcc tcatggaaat aacgtaaact gcgatggccc
       61 gaaggattca agcagactcc agatgaaact agatgccgcc tcggcatgcc tcgaaagcct
      121 cggcaacagc acccggctcg ccatctacag agccctcgtg cgtgccggac cggccggctt
      181 gcccgtcgga aagcttcagg ctcgtctgga cattcccggg tccacccttt cgcatcacat
      241 cgctcacctg gtgaaaaacg atctggtctc ccaggcccgc gagggccgcg tgcttcgctg
      301 cacggcgaac tacaggacca tgaacgatct gatcacctac ctgtccgacg agtgctgtcg
      361 ggacaccccg aggaccacct gatggctcgc cggcttgcag gcgagttcat gggcaccctg
      421 ctgctgctgg ccaccgtcgt gggatcgggg atcatgggag agcagctttc cgggggcaac
      481 gcggccatcg ccctgctcgg caacaccatc gccaccggcg ccatcctggt ggtcatcatt
      541 ttgatgttcg cgcccgtctc cggagcccac ttcaaccccg cggtcacgct cacgttcctc
      601 ctgcggcgcg agatccccgc gggtgcggcg gccgcctact gcgtgaccca ggtggccgct
      661 gggctggccg gcgtgtggct ggcccatggg atgttcgggc tggaaatcgt tcagacatcc
      721 gagaattccc ggtcaggctt cgggcaatgg ctggccgagg gggtcgccac cttcggcctg
      781 gtagcgacca tactcgccgt gctgcgctcc cgtcccgacc tggtagcgtt cgccgtgggg
      841 ctctacatct ccgccggcta ctggttcacc gcgtcgacgt ccttcgccaa ccccgcagtg
      901 accatcgccc gggcgttcac ggacacgttt tcgggaattc agcccgacca cgtccccgca
      961 ttcatcgcag cccagctcgt cggagccgcg ctcgcaacct ggacgttcgg ctggctgctc
     1021 tccgagggcg aaaggaccaa ctaaccgtcc cgctgaggct tcgagcttgc tgcggccacc
     1081 cgcgagcgaa agtcctcggt gctgttcacc cagggatatg gctcgtcggg caccggcaga
     1141 tcaagaaaat cgcacaacgg tccccaaccg gcactcgcct cgaagacgag gagcttttcc
     1201 ggcggcagcg catcgatgac gtgctggccg tgggcttcgt agatcgcgat gacgtgctct
     1261 ttgtcatcca tgcgcccgtc gaacagacca tcccagatca cctcgaacac catgtccatg
     1321 tgctcgcgga tcgccggatc cgcctgctcg cgccgggcaa cgctcgacgg atagatcgtg
     1381 ttcatgacgc tggcgtacca gcgctcgggg tcccggcggg tgaggatgac cttggcgtcg
     1441 gcaaagtgct gaagctgcgc ttcccagaag ttgcaggagg gccagtccac cgaagcctgg
     1501 tagccggcaa agaggccgtc ccagcccacc gactcgcccc gtgccgccgc ccgccagacg
     1561 gccgcgtgac cctcgttctg catgacctcg agcatgtggt aacacttctc cagccccagg
     1621 cgttccaggg cgagcttgag cgagagcgtg cccgttcggc cgaagcccgc gccgatgact
     1681 ttcagcgtca cgatgagttc ctcgaccttg tcctcacggc tggtcggcga ccgtgaccgt
     1741 gatcaactcg gacatcaccg ggggatcgtg aggcacgtgc agataattgc ccagcatgag
     1801 ctgcaggcgg taggttccgg ccggaaggct gagctgtgtt tccgtctggc cgccgccgaa
     1861 gtggcgaacc tgatccgtgg caggcagcgg cacttcggga ttcaccgctt cctcgagatt
     1921 gatcaggagg tggtggtggc cggtgttggg cgcgtccacg ccggccgggg cgacgcccat
     1981 gcctttcagg ccgaacttca ccgtgaacgg cccactcacc acggcgccat cggcgggggc
     2041 tatgaaatag agcgacgccc cttctggtgc cggcgagcgg atgccgtccg ccagcacggc
     2101 cgccgacgtc accagagcca gcgccaaggc aattgatctc aagccgttca tcgtgatcct
     2161 ccgtcgatgt tttcaggttt gccgtgcccg cccgtcggcc tccgggatca ggcggaacaa
     2221 cgggatgacc cgtcctgccc gctgctcgta gcgtcggtat cccgggtaga tggcgaccgc
     2281 cgactcccag gccgcgtctc gatccgcccc gctgagctcc tccacccgga aggtgccctc
     2341 gcggccggcg agcaggcagc tgacccggcg ctgggcacgg agattgtgac accaggccgg
     2401 atcatacggc tttccgccgt tggaacccac gagcagcgcc ccgtcgccct cgggaacgta
     2461 gagcagcggc acctccctga gcaggccgct gcgcgccccc gtgcaccgca acagcaacgt
     2521 cgggatcacg aagctgaggc ggatgcgtcc ggaagttgcc cgcatgaggg gtctgtcgac
     2581 ccaggtggcg agattcatga agaatacggt caccggcttg agcgacacga agagggaaaa
     2641 tccccgccag acggcgtccc gaatggtcac gtcaggcgat aggctcgagc ggccgtgtcg
     2701 tgaaagagcg aagccttctc cgccggagac ccatcggcca ccatcttctt gaaggcattc
     2761 cagagcacgt gataggagca gctctgcttg tccaccggga agttgctttc gaacatgcac
     2821 ctgtccacgc cgaagcagtc gatggcatgc cggtagtagc ggccggtcgc cgccacgatc
     2881 tcatcggagg tagcgggccg ttcacgcttg tgaaacccga agccgttgac gggcatgacc
     2941 aggccaccga gcttggcgta gacgttgtga cactcggcca gcgcagccac gtcgcgctgc
     3001 caggcggcga aaatctggtc ctgccggcct tcgtaggggc cgatgccgag gggcccgccg
     3061 aagtgatcga agatgatcgt cgtgtgcggg aaagctcgcg ccaggtcggt gagctggggg
     3121 atctgtgggt ggtagagcca ggcgtcgaag ctcaatccgc gcttgccgag ctccgcaaaa
     3181 cccgcccgga attcgtcgtc gagcaacaaa ccctcggacg ggttggtgtg ggagttgcga
     3241 accttctcgc tgggatccca gccggcggcg tgacgaatcc ccttgaaccg gtcgctcgcc
     3301 gccatgtggg cgtcgagcac ggggcccgca gaggatccga gggtcaggtc tacgtagccg
     3361 acgataccgg cacacacccg ggcgggcccg taatcgccgc ttgctgacat ggccgcgacg
     3421 ccgttgacga actccgtttc acccagggat ctcagctcct cgggtccgtc ggctcgatac
     3481 atgctggcac actccacgaa aaccgtagct accacgttgt gcccgctgcc cgtgtcggca
     3541 agcagctcgt cgagaaggta ccggctccca ggatggtccc acaggtgatg gtggggatcg
     3601 cagatgggca gatccggttc gaggacgtcc tcgacaacct gattcagcca gctcgcctgg
     3661 tcgcgggcca tgcggtctct ccgggaatga ttcgatttgc gacgaggcta tcgcttcgca
     3721 ggcgaaaagc aataccacgc gaatggatgg cgtctgctac gctttgccgt gtgcgcttcg
     3781 agctgagagt caaaaactgg cgcagctaca ttccgattct cgatgtgatc acccactatc
     3841 gacgatcgga tttcagccgc gacctgacgt caggactggt ggtcggcgtc atcacgatcc
     3901 cccaggccat cgcctacgcc ttccttgcgg gcctccccgc acaggcaggc ctgtacgcgt
     3961 gcctggtgcc catggtgatc tacgcaattc tcgggtcttc ccgacacctg gtagtggggc
     4021 cggtcgcggt ggcagcgctg atggtggcgg ccgccgtggg cgagcacgcg ccgctgtaca
     4081 acgagtccta cctcggcatt acggccgtgc tgggcctgca ggccggcata ttcctctggc
     4141 tgctgaggct ttggaagatg ggcggcgtcg tcaatctcct cagccatccc gtgatcgtcg
     4201 gcttcgtcaa cgcggccgcg ctgctgatca tagtcagcca gctcaacgcg ttcaccggca
     4261 ttgccgacga aggcagcaag aatccgatag atcgcctcgg tcagctcgtc tcgaagctgg
     4321 gcgagctgca cacaacgacc acgatcattg gcgtgtgctg tgttgtcggg ctctggctgg
     4381 tacggcgctg gggggtgtct gtcgccgagc tcctggtgca cccgctgggc ttcagcctgg
     4441 gcgaccggca actgatcgcg cggctggggc cactcctggt ggcctcggtc gcggtgatcg
     4501 tcgtctgggc aggggatctg aacggcacgg caggcgtcgc caccatcggc ctggttcccg
     4561 gcggtctgcc gtcactcacc gtgccgcctt tcaactggga catgtgggta gacctcgccc
     4621 cgtcatccgc gatgatcgcg gtggtcgcct acgtcgaaag cttctccatc gggaccatga
     4681 tcgcgacgcg gcagcgaacg cggatcaatg cgcaccagga gctcatcgcg ctgggcgccg
     4741 ccaacatcgg cgcggctttc acgggcgcct atccggtggc cgggagcttc tcccgatcaa
     4801 gcgtcaacta ccagaccgga gcgagaacgc cagtcagctc gctggtctgc gcggcggtga
     4861 tcattctcac gctgctgttc ttcaccccgc tgttcaccta cctgccacat gcggcgctcg
     4921 ccgccatcgt catcgtatcc gtaacgagcc tgctcgactt ccgcagcttc cgcacacact
     4981 ggcggataca ccgtgaagac agcgtgaccc agatggtcac cctggcctcg gtgctgcttt
     5041 tcggcgtgga agccgggctc ctcacgggcg tcactctttc catcgcgttc ttcatccgtc
     5101 agtcc
//
LOCUS       DBNHQR010000275         1218 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1165098, whole genome shotgun sequence.
ACCESSION   DBNHQR010000275 DBNHQR010000000
VERSION     DBNHQR010000275.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1165098"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..1056
                     /locus_tag="AC16VR_06295"
     CDS             169..1056
                     /locus_tag="AC16VR_06295"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF044908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR6010803.1"
                     /translation="MTQDTDQSRRAARLLEVLDLECIEQNLFLGANEVRGEFRLFGGQ
                     VLSQSLRAACLTVEGRSAHSLHGYFMRAGNAGRPVLYEVDRIRDGRSFATRRVVAIQS
                     GEAIFSLDVSFQVDEAGFEHAHPMPNVPPPEELEGDIAVVSRLTGDDPHLSPMAGRPR
                     PFELRSVFKPGSDEWAANRHWNPVWIRFAGEVDPDDDPLARSLLAYASDVGLVSTGVL
                     PHVESISRAEVRMASLDHALWFHRAVPIADWLLVHKRTTMARGGRALIQAEIFSRDGK
                     LVASVSQEGLIRRRSAAPG"
BASE COUNT          189 a          399 c          422 g          208 t
ORIGIN      
        1 ggtgagtcaa acagggcggc aagcttactg gcatccgtgg gcaggttcaa cgcctgggcc
       61 ccacgagacc gcgtcgggag ccgccgccgg gcgcgcggcc ggcgcccgca ggatccggtt
      121 accggccgtg ggtattcgtc attccctcgt gaatctacaa tgcacgccat gactcaggac
      181 acggatcaat cgcgacgcgc ggcgcgcctg ctggaagtgc tggacctgga atgcatcgag
      241 cagaatctct ttctgggtgc caacgaggtc cgcggcgagt tccgcctctt cggcggacag
      301 gttctgtcgc agtccctgcg ggccgcctgc ctgaccgtcg agggccgctc ggctcactct
      361 cttcacggtt acttcatgcg cgcgggcaat gcggggagac ccgtgctcta cgaggtggac
      421 cgcattcgcg acggcaggag ctttgctacc cgacgcgtgg tggccatcca atcgggggag
      481 gcgatcttca gcctggacgt ctccttccag gtcgacgagg cgggattcga gcatgcgcac
      541 cccatgccca acgtgccgcc ccctgaggag ctggaggggg acatcgccgt ggtgagccgg
      601 ctgacgggag acgatccgca cttgagcccc atggccggcc ggccgcggcc gttcgagctt
      661 cgctcggtgt tcaagccggg cagcgacgag tgggcggcaa accggcattg gaaccccgtg
      721 tggatccgct tcgcgggcga agtggatccc gacgatgacc cgctggcccg gtcgctgctc
      781 gcctacgcct ccgacgtggg cctggtctcg accggggtcc ttccccatgt ggagtcgatc
      841 agccgcgccg aggtgcgcat ggcgagcctg gaccacgcgc tgtggtttca tcgggccgtg
      901 cccatcgccg actggctgct cgttcacaaa aggacgacca tggcccgggg cgggcgggcg
      961 ctcattcagg cggagatatt ttcacgagac ggcaagctgg tcgcttccgt atcccaggag
     1021 ggtctgatca gacgccgcag cgccgccccc ggctagtcgt cgaagtagcc gacctcgagc
     1081 acggaaaagc tgagtttcgt tgcgcccgtg atcagggcca gggccgcccc gcgattgctg
     1141 ggcagcggta tgtgggtggc gtcgacgcga agcaggttcc atgtcgggtc caccggccgc
     1201 cacgtctcgt ccaaggac
//
LOCUS       DBNHQR010000276         3735 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1230036, whole genome shotgun sequence.
ACCESSION   DBNHQR010000276 DBNHQR010000000
VERSION     DBNHQR010000276.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1230036"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="AC16VR_06300"
     CDS             <1..369
                     /locus_tag="AC16VR_06300"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6010804.1"
                     /translation="WAIFPAGGSTVRLPVQLPYARAMELLLTGDLISAEEALNIGFLN
                     YVTDDIMGKAMEIAEKIAANGPLAVKAIRRSVKECLGKPEREALKIESEISGPVFRTE
                     DAKEGPRAFMEKRAPEYHGR"
     gene            complement(789..1031)
                     /locus_tag="AC16VR_06305"
     CDS             complement(789..1031)
                     /locus_tag="AC16VR_06305"
                     /inference="COORDINATES: protein motif:HMM:NF042903.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HIR6010805.1"
                     /translation="MPQEKRKRRATAAPRTDARPPAMANRPPSRQGKKPVTAYVDKQV
                     HKQLRSLGLELEKSNQEMILEALNDYFERHRLSTKG"
     gene            complement(1056..1817)
                     /locus_tag="AC16VR_06310"
     CDS             complement(1056..1817)
                     /locus_tag="AC16VR_06310"
                     /inference="COORDINATES: protein motif:HMM:NF013793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HIR6010806.1"
                     /translation="MHVVCLLSLKGGSGKSTVVQSLSVCAQQHGHRTLIVELDPQGTL
                     KNWSKRRESEQPRVVQTLPQSVGDVLEEAKAEGVHWVFLDTPGHHVASASAAAELADL
                     ILIPCKIQSVKDFDAVLPSLAEARRADKPAYVLMNQVPPNSPRLIRRRQLQIQKHYDI
                     AVLSRFLCRRADFEYCDERGLSAAELNPSGPAAEEIDRLYALLQSIFIMRSLKVDKQA
                     LVPAPGTSVGPVSRSSEVEPRPLERGQARTEELTQ"
     gene            complement(1979..2569)
                     /locus_tag="AC16VR_06315"
     CDS             complement(1979..2569)
                     /locus_tag="AC16VR_06315"
                     /inference="COORDINATES: protein motif:HMM:NF039725.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6316 family protein"
                     /protein_id="HIR6010807.1"
                     /translation="MPGKLPQWFRADEVYAYSGQWYFGSREGLHIGPYADREVAKAKG
                     TQVAKRLRAMDEAHQLKFVRKVLDREWDSVSKLIPTTGIGDAAAGSEPPPKEAVRHGE
                     GYRNWYRSGRYFQADGAWFFSTREGIDVGPFETEVQAKKHERRLVALLQRTQSAEEAF
                     QIIHEYKYRPSARSVGRVWANVTPASRTRPTKPRWA"
     gene            complement(2745..>3735)
                     /locus_tag="AC16VR_06320"
     CDS             complement(2745..>3735)
                     /locus_tag="AC16VR_06320"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR6010808.1"
                     /translation="DRPGNLPDSLIAKLPKLDNRAMGEMLGAYERENCFYMEMAGSLP
                     LSTPRMYYGDFDRDRASEKQEDILRLFDRMPRFLLSFLPALGLRIAAAKKRRYILLLE
                     DLSDAEPGDQVAGVSVRRCERALRAIARAHAACWGAELVDAFWLLPHDIDARMRHSMF
                     KGSRSTFSDFFGPELEARLEPYLRRLPDQGLGLIRRLNQAPTTLLHCDLRLDNLFFRE
                     DEVIVIDWQLVRRGPAVYDVAYFLSSALDEAAGRAVVEGLLEAYLEELQAGGVDGYTM
                     DELLRDYRIALRVVLMTLSTVDQVELGDGRGIDLMRGWISRLHGRLAEMGEPG"
BASE COUNT          682 a         1208 c         1153 g          692 t
ORIGIN      
        1 tgggccatat tccccgccgg cggatccacg gtgcggctgc cggttcagct gccctacgcg
       61 cgcgctatgg agctgctgct gaccggtgat ctgatctctg ccgaggaagc cctgaacatc
      121 gggttcctca actacgttac cgacgacatc atgggcaagg cgatggaaat cgccgagaag
      181 atcgcggcga acggaccact cgcagtgaag gcgatccgac gttccgtgaa ggagtgcttg
      241 ggcaagcccg agcgcgaggc tctaaagatc gaatcggaga tatccggacc cgtgttccgt
      301 accgaggatg ccaaggaagg gcctcgagct ttcatggaga agcgggcgcc cgaataccac
      361 ggacgctgac aacgacgggc taagcgaagc gctccccgac gggtctcggc gcgcggcgaa
      421 gctcactgct gccgcccgcc agcctgcgct ctcggctttt gcgcatctga gcagctgccg
      481 gaggcgccca gactgctcgc atctcgtcgt cctgccagac ggcgaatctg gaacctatgc
      541 gccttcctgc ccgaatcgca tcggtcagcc tctcgaagac agggccgtcc gatgagcagg
      601 tcaaggctgg aaaccggagc ataaaagcca agcgacacct ccttactgag gagacacagc
      661 atactctctc gaggccttca cgatttcgaa tcccgtgtgc agatcgtatt cgcatcgtaa
      721 tgccaacgta acggaacgtt cccgctggcg gcgaccccgt cagcggaact cgtggtcgct
      781 gtgatgcctc agcctttagt gctcagccgg tgccgttcga agtagtcgtt caaggcttcc
      841 aggatcatct cctgattgga cttctcaagc tccagaccca gcgacctcaa ctgcttgtgt
      901 acctgcttgt cgacgtaggc ggtcacgggc ttcttgccct gcctgctcgg cggccggttg
      961 gccatggccg gtgggcgggc gtccgtgcgc ggagcagccg tggcccgccg cttgcgcttc
     1021 tcctgcggca tgtgtccttc cctgtgtgtt gttgtttact gggtgagttc ttcggttctt
     1081 gcctgcccgc gctccagcgg ccggggctct acctcgctcg agcggctgac cggcccaacc
     1141 gatgtgcctg gtgcgggaac cagcgcctgc ttgtccacct tcaagctgcg catgatgaag
     1201 atcgactgca gcagcgcgta caaccggtca atctcttccg cagccgggcc ggaggggttg
     1261 agctccgcag ccgacagtcc gcgctcgtcg cagtattcga agtcggctct ccggcacagg
     1321 aatcgcgaca acacggcaat gtcgtagtgt ttctgtatct gcagctggcg acgacggatc
     1381 agccgcggcg agttgggcgg cacctgattc atcaggacgt aggccggctt gtcagcccgg
     1441 cgcgcttcgg cgaggctcgg cagcacggca tcgaagtcct tgaccgactg tatcttgcag
     1501 ggaatcagaa tcagatctgc aagctccgct gcggcggatg cagacgcgac atggtgaccg
     1561 ggcgtgtcca ggaacaccca gtgcacacct tccgctttag cctcctccag aacgtcgccc
     1621 accgactgcg gcaacgtctg aacgacgcgg ggctgttcgg actcgcggcg tttggaccag
     1681 ttcttgagag ttccctgggg atccagctcg acgatcaggg tgcggtgccc gtgctgctgc
     1741 gcgcaaaccg acagcgactg caccaccgtg gacttccctg atcctccttt gaggctcagc
     1801 aggcagacaa catgcacaat caactccgtt tgtcgacatg tatacatgtc accgtaacac
     1861 acgttccagc cgcttgttaa cccacgtttt tgtaaggtaa aagtacttat cgcgctggcg
     1921 acacggcaac catgcgtaaa agaaacggag ttaaagagcg ggaagaccgg gccgggatct
     1981 atgcccagcg cggcttcgtc ggcctggttc gactcgcggg ggtgacgttc gcccagaccc
     2041 tgcccaccga ccgcgccgag ggacgatact tgtattcgtg aatgatctga aacgcctctt
     2101 ccgcggattg ggttctctgc agaagcgcaa ccagccggcg ttcatgcttc tttgcctgaa
     2161 cttccgtttc gaagggaccg acgtcgatac cctcgcgggt agagaagaac cacgcgccgt
     2221 cggcctggaa atagcggccg gaccgatacc agtttcgata gccttcgccg tggcggacgg
     2281 cttccttggg aggaggttcg gagcccgcgg ctgcatcgcc gatgcccgtg gttggaatca
     2341 gcttggagac gctgtcccat tccctatcca ggaccttgcg gacgaacttg agctggtgcg
     2401 cctcatccat cgcgcgcagg cgcttggcta cctgggtgcc tttcgccttg gcgacttccc
     2461 gatccgcata gggcccgatg tgaagcccct cacgcgagcc gaaataccac tgaccgctgt
     2521 aggcataaac ctcatcggct ctgaaccact gcggcagttt tccgggcacg ctgggctcca
     2581 acgtggactc gatcacccga ttctaacgtc gcgacctcgt tcgccgggcg agctgcgtcg
     2641 cgttcagtgt gtgacgcagc gcaaacttcc ggccggctcg gagaggggca acaggccccg
     2701 tcgccgtgcg cgggccctca cgacgacggc cgcgcaaacc tgattcaccc cggctctccc
     2761 atttctgcga gccggccgtg caggcgggag atccagccac gcatcaggtc gatgccacga
     2821 ccgtcgccaa gttccacctg gtccaccgtc gaaagcgtca tcagcacgac gcgcagggcg
     2881 atgcgatagt cgcgcagcag ctcgtccatc gtgtacccgt ccacgccgcc cgcctgcagc
     2941 tcttccaggt aagcctcgag caagccctcc acgaccgcgc ggccggccgc ctcgtccagg
     3001 gcgctggaga gaaagtaggc cacgtcgtac accgccgggc cccgccggac gagctgccag
     3061 tcgatgacga tcacctcgtc ttcacggaag aacagattgt ccagtcgcag gtcacagtga
     3121 agcagcgtgg tcggtgcctg gttcaagcgg cggatcaggc ccaggccctg gtcgggaagg
     3181 cgtcgcagat agggttccag gcgcgcctcc agctcggggc cgaagaaatc cgagaatgtc
     3241 gatcgcgagc ccttgaacat gctgtgccgc atgcgcgcat cgatgtcgtg ggggagcagc
     3301 cagaacgcgt cgacgagctc cgctccccag cacgcggcgt gggcgcgggc aatggcgcgc
     3361 agagcccgtt cgcagcgtcg cacgctgacg cccgccacct gatcgcccgg ctcggcatcg
     3421 ctgagatctt cgagcagcag aatgtagcgc cgcttcttgg ccgcggcgat ccgcagcccc
     3481 agcgcgggaa ggaacgagag gagaaaccgc ggcatgcggt cgaagaggcg gagaatgtct
     3541 tcctgcttct cggatgccct gtcgcggtcg aaatcgccgt aatacatgcg aggggtgctc
     3601 agcggcaggc tccccgccat ttccatgtaa aagcagtttt cgcgctcgta cgcaccgagc
     3661 atctccccca tggcgcggtt atccagcttc ggcagcttgg cgatcagcga gtcaggaagg
     3721 ttcccggggc gatcg
//
LOCUS       DBNHQR010000277         2641 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_468926, whole genome shotgun sequence.
ACCESSION   DBNHQR010000277 DBNHQR010000000
VERSION     DBNHQR010000277.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_468926"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="AC16VR_06325"
     CDS             complement(<1..357)
                     /locus_tag="AC16VR_06325"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR6010809.1"
                     /translation="MAVASNISFSEKVAQGFMRAPSGAGGGLPEFLGVKITDAGPGTM
                     TGQFEVRPELLTPIGNMHGGVLSAFCDHMLGCVCYPAMKPGQWAATTEFKINLTAPVT
                     QGVVEARAEIVNMSRTQ"
     gene            complement(366..1349)
                     /locus_tag="AC16VR_06330"
     CDS             complement(366..1349)
                     /locus_tag="AC16VR_06330"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6010810.1"
                     /translation="MKYGLILGSPSIQGQIDTARRAEAAGFESVWTIEFFNMHGLVRL
                     AAVATATERVKLGTAIAYAFMRTPMLAASAAMDIDEISGGRMILGLGSGTERMNRDWY
                     SMPFDDPPAPRIKDAIGLVRAAFAAQKGGGLVYEGPYYQVRIPAYARPGAVRESIPVC
                     LAAVNRGMIRAAAAVADGLIGHPVYTRRYITDVVLPELENGSCELIPYIITAVADTTE
                     QARNEARGQIAFYYTTRLYHSILEPHGWRETGEAIAAAFRKGDFSAMADAVSDEMVDA
                     IAIAGTPDEVRDQITQWEGLADHVILYSPSVGMRGGRVQENLDAIVDTFAA"
     gene            complement(1379..2461)
                     /locus_tag="AC16VR_06335"
     CDS             complement(1379..2461)
                     /locus_tag="AC16VR_06335"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR6010811.1"
                     /translation="MSFAMVQTDVRKLEPMELPLPEIDEDSALLKLEACGICGSDHEQ
                     YEGLLRTPMPVVPGHEPLGTIAKIGDRAATRWGVDQGDRVAVETMISCRHCRPCLSGS
                     YHLCDHRLIYSYVPVSEAPGLWGGYSQYMYLHPNSIVHRVSPELPAEIGVMFNPLGAG
                     FRWAVELPATRPGDTVVILGPGQRGLASVIACREVGAGTIIVTGLSADEKKLQLAREF
                     GADYTIDVDSEDTVKRVKAITDGRGADVVVDVSAYATKPVSDALSLVRPGGTVVLAGV
                     KGFKAVEDFVSDLVVLKEINIKGAIGVTSSGYRSAIRLLESGRVPVERMHTHDFALAD
                     AELAIRTLAREVPGDESVHSCLIPPH"
BASE COUNT          458 a          928 c          808 g          447 t
ORIGIN      
        1 ctgagtccgc gacatgttga cgatctccgc acgagcctcc acgacgccct gggtgaccgg
       61 tgccgtgagg ttgatcttga actcggtggt ggccgcccac tggcccggct tcatggccgg
      121 atagcagacg catcccagca tgtgatcgca gaaagcggat agcacgccgc cgtgcatgtt
      181 gccgatgggc gtgagcaatt ccggcctgac ctcgaactgc ccggtcatgg tgcccggacc
      241 cgcgtccgtg atcttcacgc cgaggaactc gggcaaaccg cccccggccc cggacggcgc
      301 cctcatgaac ccctgagcca ccttctcact gaaactgatg ttcgacgcaa ccgccatgtt
      361 ttacctcaag ctgcaaacgt gtccacgatg gcatccagat tctcctgaac ccgaccgccg
      421 cgcatcccca cgctgggcga gtagagaatg acgtggtcgg caagaccctc ccactgcgtg
      481 atctggtcgc gcacctcgtc cggcgtcccc gcaatggcga tggcgtcgac catctcgtcg
      541 ctcaccgcgt ccgccatggc ggaaaaatct cctttgcgga aggcggccgc aatggcttct
      601 cccgtctccc gccagccatg gggctctaaa atggagtggt aaagccgcgt ggtgtagtaa
      661 aaggcgatct gcccgcgcgc ttcgttgcgc gcctgctccg tcgtgtccgc caccgccgtg
      721 atgatgtaag ggatcagctc gcacgagccg ttctccagtt ccggtaagac cacgtcggtg
      781 atgtacctgc gggtgtagac cgggtggccg atcagcccgt ccgccacggc ggccgcagcg
      841 cggatcatgc cccggttcac ggccgcgagg cacaccggga tcgattcccg cacggcgccg
      901 gggcgcgcat acgcgggaat cctgacctga tagtaagggc cttcgtagac aagccccccg
      961 cccttctgag cggcaaacgc cgcccgcacg aggccgatgg cgtccttgat cctgggcgcc
     1021 ggcgggtcgt cgaacggcat ggagtaccag tcgcggttca tcctctcggt gccgctgccc
     1081 aatcccagaa tcatgcgccc gccggaaatc tcgtcgatgt ccatggcggc agatgcggcg
     1141 agcatcggtg tgcgcatgaa ggcatacgcg atggcggtgc ccagcttgac gcgctccgtc
     1201 gccgtggcca cggcggcaag gcgcaccagc ccgtgcatgt tgaagaactc gatggtccaa
     1261 acggattcga agcccgcggc ttccgcccgg cgagccgtgt cgatctgtcc ctggatggag
     1321 ggagatccca gaatcaggcc gtatttcatg acgtcttctc tccgtggcaa tccgggtttc
     1381 agtgcggggg gatcaggcag gagtgaacgg actcgtcgcc aggaacctct cgagccaggg
     1441 ttcgtatcgc aagctccgcg tccgccagcg cgaagtcatg ggtgtgcatg cgttccaccg
     1501 gaacccgccc cgactccagc aggcggatgg cgctgcgata tccggacgac gtcacgccga
     1561 tggccccctt gatgttgatc tccttgagca ccacgagatc cgagacgaaa tcctccaccg
     1621 ccttgaagcc cttcacgccg gccagcacca ccgtgccgcc cggccgaacc agcgacagcg
     1681 catcggacac gggtttcgtc gcgtaagcgc tcacgtccac caccacgtcc gcgccgcgcc
     1741 cgtccgtgat cgccttgacc cgctttaccg tgtcctcgct gtccacgtcg atggtgtaat
     1801 ccgccccgaa ctcccgggcg agctgaagct tcttctcgtc tgcggaaagg cccgtcacga
     1861 tgatggtccc ggcccctacc tcccggcagg caatcacgct ggcaagcccc cgctggccgg
     1921 ggccgagaat gaccaccgtg tctcccggcc gggtcgccgg cagctccacc gcccagcgga
     1981 agcccgcgcc aagcgggttg aacatcacgc cgatctcggc cggcagctcc gggctcaccc
     2041 gatgcacgat gctgttcgga tgcagataca tgtactgtga gtagccgccc cacagccccg
     2101 gcgcttcgct cacaggcacg tacgagtaga tgagccggtg gtcgcagagg tgatacgagc
     2161 cgctcaggca cggccggcag tgacggcagg agatcatcgt ctccaccgcg acccgatcgc
     2221 cctgatcgac gccccagcga gtcgccgccc ggtcaccgat cttggcgatg gtgcccaggg
     2281 gctcgtgacc gggaaccacg ggcatgggcg tgcgcagaag accctcgtac tgctcgtgat
     2341 cgctgccgca gataccgcag gcttccagct tcagcagcgc cgaatcttcg tcgatttcag
     2401 gcagcgggag ctccatgggc tcgagcttgc gcacgtcggt ctgaaccatc gcgaacgaca
     2461 ttctcggcaa cgtcaacggt gcggctcccc ctcgctcgaa gcgctcggtc gcgcctcctc
     2521 ccgtgatagt ctccacgaga ctacgggcaa cggcctgaca gagcaatggc aagaaccccc
     2581 accgtcatcg tcgtcggcgg cggcaacgcg gccctttgcg ccgcgatcag cgcggccgag
     2641 c
//
LOCUS       DBNHQR010000278         3934 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_279539, whole genome shotgun sequence.
ACCESSION   DBNHQR010000278 DBNHQR010000000
VERSION     DBNHQR010000278.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_279539"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1437
                     /locus_tag="AC16VR_06340"
     CDS             <1..1437
                     /locus_tag="AC16VR_06340"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6010812.1"
                     /translation="QLANGFCAQGFEKGARIGVLARNSIEYFEVYYACARAGLIAQPL
                     NWRLGVDELARILEDGGPSAIVTSAEFRGERDALQGLVDVPRWLEFGASSDGTFEDLV
                     RASSAEEPQRSDSTGDDDPVLILYTGGTTGLSKGALHSHRSLFMGMVNQTVAERVVPT
                     DVYMLTGQMFHIPVGLAMNYMAHGCPVVLMNFEARQALEIIQAERVSAFLGITTMLNW
                     MMAVDDFASYDLSSLRNIQYGGGPMPGTVVKAALESFPCTLIQGYGQTEGMTMTFLSQ
                     EDHADAVAGKNSERLNSCGREGFVTRVQVVNEQGEPVPKDGATAGEIVVRSDANMLGY
                     WGRPDLTAQTIRNGWMWTGDVATWDEDGYVFIVDRAKDMIISGGENIYSTQVEAAIHK
                     HPAVLEAAVIGGPDDEWGEAVKAVVVLKPGETATEQEIIDTARENLASYQKPKSVDFV
                     DSLPKAPTGKILKRELRDPYWKDQDRNV"
     gene            complement(1434..2615)
                     /locus_tag="AC16VR_06345"
     CDS             complement(1434..2615)
                     /locus_tag="AC16VR_06345"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6010813.1"
                     /translation="MSGPLSGIRIIELATMIAVPGATHLLATQGAEVIKVEDPVRGDE
                     LRHYGSEKGGMSAWFINANSGKRSIALDLQHEDGKSVLWRLLETADVVMQGFRPGVIG
                     RLGFDSETVRDRLPAIVYCSSSGFGPHGPYAQLPVYDPVIQALSGWAGAQRYDEGPRL
                     VRGMVADKVAALTSAQAVTAALLQRSMTGRGLHVELSMLEANIAFNWSDVMMHASLLD
                     ADATHRPNLLSSYRLFPCRDGWVTVTAGTDAQWRAFSQALERPELAEDARFVTASARA
                     GHVADWYEAMGEAVAAFRLQEVLDRLRTADVPVAPVLDPTEVAEDPQVLAAGLLREVD
                     HPVAGRLRQPRPSAEIFGSSQDLIPAPTHGQQTAQVLAEIGYDEGAIGRLAADGVVKV
                     D"
     gene            complement(2641..3456)
                     /locus_tag="AC16VR_06350"
     CDS             complement(2641..3456)
                     /locus_tag="AC16VR_06350"
                     /inference="COORDINATES: protein motif:HMM:TIGR03084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03084 family metal-binding protein"
                     /protein_id="HIR6010814.1"
                     /translation="MSTAEQIELARQVDDLRAEGAELAELMSKLTEEDWQRRTTFKDW
                     TIYDVIAHLHFSDHMGMTTIESGAAFQALMADMQSSGLPFVEYTRRWLGSVSGADLLE
                     RWKTLLDELCDRLQALDPQERLTWAGPGMKPRMFTTARQMETWAHGWEIYDCLRLERM
                     HTDRIKNIAAIGVRTFGWTFVNRGLELPGDPPFVRLTAPSGAIWEWSDPNADSSVTGS
                     AVEFCQVVTQVRNVADTKLEVTGKPAREWMAIAQCFAGAPEDPPAPGSRRPAG"
     gene            3608..>3934
                     /locus_tag="AC16VR_06355"
     CDS             3608..>3934
                     /locus_tag="AC16VR_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6010815.1"
                     /translation="MTEPAAVRAEIPDPYSLPLDTLNVAQPALFQNDAHWGYFERLRN
                     EDPVHYCPQSDFGPFWSVTKFKDIFYVDTHHEIFSSEGGITLGPPVNQPPEEGFRPSM
                     FIAMDPP"
BASE COUNT          695 a         1265 c         1287 g          687 t
ORIGIN      
        1 cagctcgcca acgggttctg tgcccagggg ttcgaaaagg gcgcgcgaat tggcgtgctg
       61 gccaggaaca gcatcgagta cttcgaggtt tattacgcct gcgcgcgtgc gggattgatt
      121 gcccagccgc tcaactggcg cctgggggtg gacgagctgg ctcgcatcct cgaggacggc
      181 ggcccaagcg ccatcgtcac gtccgccgag ttccgcggcg agcgggatgc gctgcagggg
      241 ctggtggacg tgccgcggtg gctcgagttc ggcgcttcga gcgacgggac ctttgaggat
      301 ctcgtgcgcg cgtcgagcgc cgaggagccg cagcggtcgg actcgaccgg ggacgacgac
      361 ccggtattga tcctgtacac gggagggacc acggggctgt ccaaaggagc cctgcactcg
      421 cacagatcgc tcttcatggg catggttaac cagacggtgg ccgagcgcgt ggtgcctacc
      481 gacgtctaca tgttgacggg ccagatgttt cacatcccgg tggggctggc gatgaactac
      541 atggcccacg ggtgccctgt ggtgctgatg aacttcgagg ccaggcaagc gctggaaatc
      601 attcaggcag aacgggtgtc cgcctttctc ggcatcacca ccatgctcaa ctggatgatg
      661 gcggtggacg atttcgcgag ctacgacctt tcgagcctcc gcaacataca gtacgggggc
      721 gggccgatgc cgggtacggt cgtgaaggcc gcgctcgagt ccttcccctg cacgctgata
      781 cagggttacg gccagacaga gggcatgacc atgacgttcc tctcccagga ggatcatgcc
      841 gacgcggtcg ccgggaagaa ctcggagcgc ctcaactcct gcggtcgcga gggcttcgtg
      901 acgcgggtcc aggtggtgaa cgagcagggc gagccggtcc cgaaagacgg cgccacggcc
      961 ggggagatcg tggtgcgctc ggacgccaac atgctcggct actggggacg tccggatctc
     1021 accgcccaga ccatccgcaa cggatggatg tggacgggcg acgtcgccac ctgggacgag
     1081 gatggatacg tcttcatcgt cgaccgcgcc aaggacatga tcatctccgg aggggagaac
     1141 atttactcga cccaggtcga agcggccatt cacaaacatc cggccgtgct ggaggcggcg
     1201 gtgatcggtg gtcccgacga cgagtggggg gaagccgtga aagcggtggt ggtgctcaaa
     1261 cccggagaga ccgctaccga gcaggagatc atcgacacgg cccgggagaa cctggcctct
     1321 taccagaaac cgaaatccgt cgacttcgtc gacagcctgc cgaaggctcc cacgggcaag
     1381 attctcaagc gtgagctgcg ggatccctac tggaaggacc aggaccggaa cgtctagtcg
     1441 accttgacga cgccatcggc cgccagcctg ccgatggcgc cctcgtcgta gccgatctcg
     1501 gccaacacct gtgccgtttg ctgcccgtgg gtcggcgcgg ggatcaggtc ctgagagctc
     1561 ccgaaaattt cggcgctcgg tcggggctgg cgcagccttc ctgcaaccgg atgatcgacc
     1621 tcgcgcagca gcccggccgc cagcacctgt ggatcctcgg cgacttcggt cggatcgagc
     1681 acgggtgcca cgggcacgtc ggccgttctc aggcggtcga gcacttcctg gagcctgaag
     1741 gcggccacgg cctcgcccat ggcctcgtac cagtccgcca cgtgcccggc acgcgccgac
     1801 gcggtgacga accgggcgtc ctccgccagc tccggtcttt ccagcgcctg gctgaaggcc
     1861 cgccactggg cgtcagtgcc agcggtcacc gtgacccatc cgtcgcgaca ggggaacagc
     1921 cgataggagg aaagcaggtt gggcctgtgg gtcgcatccg catcgaggag ggaagcgtgc
     1981 atcatgacgt cggaccagtt gaaggcgatg ttggcctcca gcatgctgag ctcgacgtgt
     2041 agcccgcgac ccgtcatcga cctctgcagc agagccgcgg tcacggcctg ggcggacgtc
     2101 agagcggcca ccttgtcggc caccatgccg cgcaccaggc gcggcccctc gtcgtagcgc
     2161 tgtgctccgg cccagccgga cagcgcctga atgacggggt catagaccgg gagctgggcg
     2221 taggggccgt gggggccgaa gcccgatgac gagcagtaga cgatggcagg taggcggtct
     2281 ctgaccgttt cgctgtcgaa gccgaggcgc ccgatgacgc caggccgaaa accctgcatc
     2341 accacgtcgg cggtctccag gagtctccag agcacgctct tgccgtcctc atgctgaagg
     2401 tccagggcga tcgagcgctt gccggagttc gcgttgatga accaggcact catgccgccc
     2461 ttctcgctgc cgtagtggcg cagctcgtca ccgcgcaccg ggtcttccac cttgatgacc
     2521 tcggccccct gggttgcaag caggtgcgtg gccccgggga ccgcgatcat cgtcgccagc
     2581 tcgatgatcc gtatgccgct caagggtcct gacatgcaaa cctccgatgc tgacgccgtt
     2641 tcaacctgcg gggcggcggc tgccgggcgc aggcggatcc tcgggagcac cggcaaagca
     2701 ttgggcaatg gccatccact ccctggccgg cttgccggtt acctcgagtt tcgtatcggc
     2761 gacgttgcgt acctgggtga cgacctggca gaactccacc gcgctgccgg tgacgctgct
     2821 gtccgcgttc ggatcactcc actcccagat agctccggaa ggggcggtca ggcgaacgaa
     2881 aggcggatcc ccgggcaatt ccaggccccg attgacaaaa gtccagccga aagtgcgcac
     2941 gccgatggct gcaatgttct taatccggtc ggtgtgcatc ctttccagcc ggagacaatc
     3001 gtagatttcc cacccgtggg cccacgtctc catctggcgc gcggtggtga acatccgggg
     3061 tttcatgccg gggcccgccc acgtcaggcg ttcctgagga tccagggcct ggagacgatc
     3121 acacaactcg tccagcagag tcttccagcg ctcgagaaga tccgcgccgg atacgctgcc
     3181 cagccatcgt ctggtgtact cgacgaacgg gagccccgag gactgcatgt ctgccatcag
     3241 cgcctggaac gcggcgccgc tttcgatggt ggtcatcccc atgtggtcag agaagtgcag
     3301 atgagcgatg acgtcgtaga tcgtccagtc tttgaaggtc gttctgcgct gccagtcttc
     3361 ctccgtcagc ttggacatca gctcggccag ctcggcgcct tcggcgcgca ggtcgtctac
     3421 ctgcctggca agctcgattt gttccgcggt gctcatgatc ttggatcccg aagctgaggg
     3481 gccatcatat accaggcgtg ttggcgcggc tcatccgccc ggcccggctc aggacgtcgc
     3541 gtagacgtcg caaagcgctg gccctaccat gaagcatccg gcgagattcc gattcgaggg
     3601 ccgacgcatg accgaacccg ctgccgtccg agccgagatt ccagatccgt attccctgcc
     3661 tctggacacg ctcaacgtgg cccagccggc gctgtttcaa aacgacgccc actggggtta
     3721 cttcgagcgg ttgcgcaacg aggaccctgt gcactactgc ccccagagcg atttcggtcc
     3781 gttctggtcg gtgacgaagt tcaaagacat cttctacgtg gacacccatc acgagatctt
     3841 ttcatccgag ggcgggatca cgttgggccc gcccgtcaac cagcctccgg aggaaggatt
     3901 ccgtccgagc atgttcatcg ccatggatcc tccc
//
LOCUS       DBNHQR010000279         2350 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_660027, whole genome shotgun sequence.
ACCESSION   DBNHQR010000279 DBNHQR010000000
VERSION     DBNHQR010000279.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_660027"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="AC16VR_06360"
     CDS             <1..285
                     /locus_tag="AC16VR_06360"
                     /inference="COORDINATES: protein motif:HMM:NF013982.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HIR6010816.1"
                     /translation="CQRALDVVNELDELIATGFRGVEVQRVEEMIDALDESESRNDDA
                     QVRLRAELHGLERELDAVDVIFLYRIIDRIGDLADNAQSVGHRLEMMLAR"
     gene            339..1592
                     /locus_tag="AC16VR_06365"
     CDS             339..1592
                     /locus_tag="AC16VR_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010489432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005315 - phosphate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HIR6010817.1"
                     /translation="MDPTTLLLLLALLFGLYMAWGIGANDVANAMATSVGARAITVKQ
                     AILIAAVFEFLGAFLAGGEVTSTIRKGIIDSSALVGDPDVLILGMLAALLAAAVWLTV
                     ASRFGWPVSTTHSIVGAIVGFAIATIGMEAVHFDKVGSIAASWVVSPLLAGTLSFLLV
                     RSVQWLILDRPNPELYARRYVPAYIFLAALNISLVTLLKGLKHIGLDLTAFEAIGYSV
                     AFALVIMAGGAVMVRRIHTATATEMEDYHYANVERTFAVLMIVTACAMAFAHGSNDVA
                     NAIGPVAAIISTVQSGEVFQKASVPPYVLLLGAAGIVIGLATYGYRVMATVGRKITEL
                     TPSRGFAAELAAASTVVLASGTGLPISTTHTLVGAVLGVGLARGISALNLGVIRTIFM
                     SWVITLPVGAVLAVLFYYMLRAALL"
     gene            complement(1626..>2350)
                     /locus_tag="AC16VR_06370"
     CDS             complement(1626..>2350)
                     /locus_tag="AC16VR_06370"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6010818.1"
                     /translation="IVRALLAEGASVAFSGRTQEKGETLMAELAMPDRTWFCQGSVTV
                     TEDIHELVDGAVERFGRLDIMVNNAGGSTDALQPVAELDEAAWESDLRFNLTSTFLGT
                     KRALKHMLPAGYGTIVNISSIEGKRGTPVMSAYCACKHGINGFTKAVAAEVGRSGVTV
                     NAICPGLIITDAITTGGPVMAETMGMTFDEMVETVFKANTLTGELNTVEQIGDMTVFL
                     ASTAGRGITGQQISVDCGMSPY"
BASE COUNT          371 a          805 c          741 g          433 t
ORIGIN      
        1 tgccaacgtg cgctggacgt ggtcaacgag ctcgacgagc tcatcgcgac gggcttcaga
       61 ggcgtagagg tgcagcgggt ggaagagatg atcgacgccc tcgacgaaag tgaatcccgc
      121 aacgatgacg cccaggtccg cctccgagcg gaactgcatg gtctggagcg ggaactcgat
      181 gccgtggacg tgatatttct gtatcgcatc atcgatcgca tcggtgatct ggctgacaac
      241 gcccagagcg tcggccacag gctggagatg atgctggcgc gttgagggcg caagcttcct
      301 ggcgctgagc cgacagaaaa accgggaaag ggatggcgat ggacccgacg acactgctac
      361 tgctgctcgc gctgctgttc ggcctctaca tggcctgggg catcggcgcc aacgacgtcg
      421 ccaatgccat ggccacctcc gtcggcgccc gggccattac cgtgaagcag gcgattctga
      481 tcgccgcggt gttcgagttt ctgggcgctt ttctggccgg cggagaagtc acgtccacca
      541 tccgtaaagg catcatcgac tcgagtgctc tggtgggcga tccggatgtt ctgatactgg
      601 ggatgctcgc cgcgctgctc gccgcagcgg tgtggctcac ggtggcctca aggttcggct
      661 ggccggtctc caccacccac tccatcgtcg gcgccatcgt cggctttgcc atagccacca
      721 tcgggatgga ggccgttcac ttcgacaagg tcggctccat cgcggccagc tgggtcgtct
      781 ccccgctgct ggcgggcacg ctctcgtttc tgctggtccg gtccgtgcag tggctgatcc
      841 tggaccgccc gaaccccgag ctgtacgccc ggcgctacgt tcccgcctac atcttcctcg
      901 ccgcgctgaa catctcgctg gtgacgctgc tcaaagggct caaacacatc ggcctggatc
      961 tgaccgcttt cgaggccatc ggctattccg tggcctttgc cctggtcatc atggccggcg
     1021 gcgccgtgat ggtgcgacgc atccacaccg caacggccac ggagatggag gactaccact
     1081 acgccaacgt ggagcgcacg tttgccgtgc tgatgatcgt caccgcctgc gccatggcct
     1141 tcgcccacgg ctccaacgac gtggccaacg ccatcggccc cgtggccgcc atcatctcca
     1201 ccgtgcagtc cggcgaggtc ttccagaaag ccagcgtgcc gccctacgtg ctgctgctcg
     1261 gcgccgcggg catcgtgatc ggcctggcca cctacggtta ccgcgtcatg gccacggtag
     1321 ggcgcaagat cacggaactg acccccagcc gcggcttcgc agcggagctc gccgcggcat
     1381 ccaccgtggt gctggccagc ggaaccgggc tgcccatctc aaccacccac accctggtgg
     1441 gcgccgtcct gggcgtgggg ctggcccgtg gcatcagcgc tctgaacctg ggcgtcatcc
     1501 ggaccatctt catgtcctgg gtgatcacgc ttcccgtggg cgccgtgctg gccgtccttt
     1561 tctactacat gcttagagcg gcgctgctct aaccgggcca tcgccgccct ggggccgggg
     1621 ccgtctcagt agggcgacat cccgcagtcc accgagatct gctgaccggt gatgccgcgg
     1681 ccggcggtag acgcgagaaa caccgtcatg tcaccaatct gctccaccgt gttcagctcg
     1741 ccggtcaggg tattcgcctt gaacacggtc tccaccatct cgtcgaacgt catccccatg
     1801 gtctccgcca tcaccgggcc gccggtggtg atggcgtcgg tgatgatcag gcccgggcag
     1861 atggcgttga ccgtgacccc tgagcggccc acttcggccg ccaccgcctt ggtgaacccg
     1921 ttgatgccat gcttgcaggc gcagtaggcc gacatgaccg gcgtgccgcg ctttccttcg
     1981 atggaggaga tgttgacgat ggtgccgtac ccggcgggca gcatgtgttt aagcgcgcgc
     2041 ttggtgccca gaaaggtcga ggtcaggttg aagcgcaggt cggactccca ggccgcctcg
     2101 tccagctcgg ccacgggctg cagggcgtcc gtgctcccgc ccgcgttgtt caccatgatg
     2161 tcgagccggc cgaagcgctc cacggcaccg tccaccagct cgtgaatgtc ctccgtgacc
     2221 gtcaccgacc cctggcagaa ccaggtccga tccggcatcg caagctcggc catcagtgtc
     2281 tcgcccttct cctgggtgcg cccggagaag gccacgctgg caccctcggc cagcagcgcg
     2341 cgcacgatgc
//
LOCUS       DBNHQR010000280         6762 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_26791, whole genome shotgun sequence.
ACCESSION   DBNHQR010000280 DBNHQR010000000
VERSION     DBNHQR010000280.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6762)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6762)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6762
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_26791"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..1035
                     /gene="pheS"
                     /locus_tag="AC16VR_06375"
     CDS             22..1035
                     /gene="pheS"
                     /locus_tag="AC16VR_06375"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010209123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HIR6010819.1"
                     /translation="MNIEELLEQARTGIAEAGDEAALDAVRVRFLGKKGELTVLLKSL
                     GTLDAEERPKAGAAINLAKEQVQEWIAQRRASLGSEQLAQALASESVDVTLPGRGERP
                     GGLHPVTQAMERIEDIFTGAGYSVVPGQEIEDDYHNFEALNIPAHHPARAMHDTFYFG
                     NGLLLRTHTSPSQVHVMENQAPPIRVICPGRVYRRDSDLTHTPMFHQVEGLVIDRGIS
                     FADLKGTVVGFLQRFFEQDLAVRFRPSYFPFTEPSAEVDVQCVHCLGKGCRVCSQTGW
                     LEVMGCGLVHPNVLEMSGVDSEEFSGFAFGMGGDRLAMLLYEVDDLRMFFDNDLRFLR
                     QFT"
     gene            1054..3432
                     /gene="pheT"
                     /locus_tag="AC16VR_06380"
     CDS             1054..3432
                     /gene="pheT"
                     /locus_tag="AC16VR_06380"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020300162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HIR6010820.1"
                     /translation="MKFSEAWLREWADPGISREALLDQLTMAGLEVDGVEPVSKEFAG
                     VVIAEVVSVDAHPNADKLSVCTVDDGTQTFQVVCGAPNVRAGMIGAFARVGAVLPDDF
                     RIKAAKLRGVESQGMLCSAAELGMGDDHSGILDLDCPDRVGDDLRQALALDDVIVDLD
                     LTPNRGDCLSVRGLAREVGVLNNVPVAAGEVEAVAPVLEDTFSVEIQDPAGCPRYLGR
                     VIRGIDPSCASPLWLTEKLRRCGVRAIDPVVDVTNFVMLELGQPMHAFDLGQLRDGIV
                     VRRARDGEQLRLLDGQELALDADTLLITDGSGPVAIAGVMGGERSGIQAATTDVFLEC
                     AYFAPLAVAGTARRYALHTDASHRYERGVDFELQHAAMERATRLLLDIVGGEPGPVVE
                     AVEPKALPEVSDIGLRERRLNRLLGTEIAPEIVDEAMERLDFAVTSRETGEEGVRWTV
                     RVPSHRFDIVREADLVEEVCRIYGYNNVPVRQPVTELELTPVPLERSPAGRLKRLLAT
                     LGYQEAITYSFVDPRLQDLLAPAADALVLSNPMSSEQSVMRLNLLPGLVEALRFNVAR
                     QQDRVRIFELGSCFAPGDPVLQKLMLGGVLWGRRHGENWNQSGAEVDFYDLKGDVEAI
                     VGWSGEPVAYERLEDPVLHPGQAALVVGSRGPVGRLGRLHPEIERALDLPGHVFIFEL
                     EADAVLHRARRRHLSLSKFPSVRRDLAVVVQADTASSSIERILRESLGEILVDFRIFD
                     VYQGKGIDSNEKSLGIGLTLQHPSATLNDQEIGKYMNDAVDALVRGVGARLR"
     gene            3496..3801
                     /gene="ihfA"
                     /locus_tag="AC16VR_06385"
     CDS             3496..3801
                     /gene="ihfA"
                     /locus_tag="AC16VR_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012613949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HIR6010821.1"
                     /translation="MGALTKAQMADRLFDELGLNKREAKEIVEMFFDEVRDSLENNQQ
                     VKLSGFGNFDLREKGERPGRNPKTGEEIPITARRVVTFRPGQKLKARVEAYAGPGSD"
     gene            3779..4135
                     /locus_tag="AC16VR_06390"
     CDS             3779..4135
                     /locus_tag="AC16VR_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002553165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR6010822.1"
                     /translation="MLDPGQTDELPPIPGKRYFTIGEVSQLCEVKPHVLRYWEQEFPQ
                     LKPVKRRGNRRYYQRQDVMTIRQIRSLLYDQGFTIGGARQQLAGADAKRDHSQFKLLV
                     RQMIVELEDLLRVLKS"
     gene            complement(4392..5645)
                     /locus_tag="AC16VR_06395"
     CDS             complement(4392..5645)
                     /locus_tag="AC16VR_06395"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6010823.1"
                     /translation="MSEIDEKEVRQEVGTWLEANWDPQLGLLEWRNILVDSGWGMPHW
                     PTQWHGRGLPVGLTPAVDDELASVNAVSVAKSGIRLLAAATLLEHGSDMHREKFLRRI
                     LTGEDTWCQLFSEPGSGSDLAGATTRADFDGTSWIINGQKVWTTSAHHAQWGLLLART
                     DWDVPKHQGLSYFVLDMSQPGVEVQPLRQMNGHASFNQVFITDAKVAPEFQVSTTGNG
                     WAVAMTTLAHERRGADGLRHAARAKDLPGRIYEEERAELATVMEPYKWYPQRAGRIDL
                     VIDRASATGALAQPNVRQAMARALIMSRSAEWTARRARAAQQLGRPQGPEGSLGKLAA
                     SNVARACSHVHTLISGTDAMLTGPESPLGGVVSEILLSVPATSIAGGTDEVQRNIISE
                     RVLGMPKEPRFDTGPFRDVPKNVLT"
     gene            complement(5657..>6762)
                     /locus_tag="AC16VR_06400"
     CDS             complement(5657..>6762)
                     /locus_tag="AC16VR_06400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6010824.1"
                     /translation="EQFREATRRFLQDKSPTPEVRRLMETSEGFDEAVWKQLSGELAL
                     PGIHIPEQYGGAGFGPVELGIIMEELGRSLLCAPYFGSSVLAATAILEAATEDWKCEL
                     LPALAAGEQLGALAVTETGGRWDSDGIATVARDSGAGFSLTGTKSFVVDGLTAGFLIV
                     AARTPDTGGEAGVGLYLVRPDDAGVTRTPLETMDATRKLARIDLLDAPARPLGEPGAD
                     WPALQRTLDLAAVALANESVGGAQVMLDSSVEYSKIRMQFGRAIGSFQAIKHRCADML
                     VEVELAKSAAYYAAQAAASGDPELPALACLAKAAASDAFVSTAASCIQIHGGIGFTWD
                     HDTHLWFKRAKSSEVFLGTPDHHRELYLQRTGV"
BASE COUNT         1107 a         2164 c         2301 g         1190 t
ORIGIN      
        1 cttttttcgt ttgagaccga gatgaacata gaagagctac tcgagcaggc caggacggga
       61 atcgccgaag caggggatga agcggctctc gacgccgtgc gcgttcgctt tctcggcaag
      121 aagggggaac tcaccgttct cctgaagtcc ctgggtactc tcgatgccga ggaacgaccc
      181 aaggctggcg cggccatcaa tcttgcgaag gaacaggttc aggagtggat cgcgcagcgc
      241 cgcgcgtcgc tcggctccga gcagcttgcc caggcgctcg ccagcgaaag cgttgacgtg
      301 accctgcccg gtaggggaga gcggcctgga ggcctgcatc cggttaccca ggcgatggag
      361 cggatcgagg acatcttcac cggagccggc tacagcgtcg tcccgggcca ggagatcgag
      421 gacgactatc acaatttcga ggctctcaac attcccgcgc accatccggc ccgggccatg
      481 cacgacacct tctacttcgg caacggcctg ctgctgagga cccacacctc gccgtctcag
      541 gttcacgtca tggagaacca ggcgccgccc atccgggtca tctgcccggg gcgcgtctat
      601 cgacgggatt ccgatctcac ccataccccc atgtttcacc aggtcgaagg gctggtcatc
      661 gatcgcggca tcagctttgc cgatctaaag ggcacggtgg tgggctttct gcagcggttt
      721 ttcgagcagg atctcgccgt ccggttccgg ccttcgtact tcccgttcac cgagccgtct
      781 gccgaagtgg acgtccaatg cgttcactgc ctgggcaagg gctgccgggt ctgcagccag
      841 accggctggc tcgaggtgat gggctgcggg ctcgtccacc cgaacgtgct cgaaatgtcg
      901 ggcgtggaca gcgaggagtt ctcggggttc gccttcggca tgggcggcga ccggctcgcc
      961 atgctgctct acgaggtcga cgacctgcgc atgttcttcg acaacgatct ccgcttcctg
     1021 cggcagttca cgtgaccggg tcggggggcg atcatgaagt tcagtgaagc ctggctgcgc
     1081 gagtgggcgg acccaggcat ctccagggag gcgctgctgg accagctgac catggccggg
     1141 ttggaggtgg acggcgttga gcccgtatcc aaagagtttg ccggcgtcgt gatagctgag
     1201 gtggtctcgg tggacgccca ccccaatgcg gacaagcttt cggtttgcac cgtcgacgat
     1261 ggcacacaga ccttccaggt ggtgtgcggc gcgccaaacg tccgggcggg catgatcggc
     1321 gccttcgccc gggtgggggc cgtgcttccg gatgatttcc gcatcaaggc ggccaagctt
     1381 cgcggagtcg agtcccaggg catgctctgc agcgcggccg agctcggcat gggcgatgac
     1441 cacagcggca tcctcgacct cgactgcccc gaccgcgtgg gcgatgacct gcgacaggcg
     1501 ctggccctcg acgacgtcat cgtcgacctg gacctcacgc cgaaccgcgg tgactgcctg
     1561 agcgtgcgag gtctcgcccg ggaagtgggg gtgctcaaca acgttccggt cgccgccggc
     1621 gaggtggagg ccgtcgcgcc cgtgttggag gatacgttca gcgtcgagat ccaggatccc
     1681 gcaggctgcc cgcgatatct gggacgggta attcggggca ttgatccctc ctgcgcgagc
     1741 ccgctgtggc tcaccgagaa gctgcgccgc tgcggcgtgc gcgccatcga cccggtggtg
     1801 gacgtcacca atttcgtcat gctcgagctg ggtcagccca tgcatgcctt cgacctcggg
     1861 cagcttcgcg acggcatcgt ggtccggcgg gcgcgggatg gcgagcagct gaggttgctg
     1921 gatggccagg aactggccct ggacgcggac accctgctga tcacggatgg cagcggaccg
     1981 gtcgccatcg ccggggtgat gggcggggag cgcagcggca ttcaggcggc aaccaccgac
     2041 gtgttcctgg agtgcgccta cttcgccccg ctggcggtag ccggcacggc gcgtcggtac
     2101 gcgctccaca cggacgcgtc gcaccgctat gagcggggcg tggatttcga gctgcagcat
     2161 gcggccatgg agagagcgac ccggctgctc ctggacatcg ttggcgggga gccgggaccg
     2221 gtggtggagg cggtggaacc gaaagccctt ccggaggtgt ccgacatcgg gttgcgcgag
     2281 cgtcggctca accgtcttct cgggacggag atcgcgccgg agatcgtcga cgaggccatg
     2341 gagaggctcg acttcgcggt gacgtctagg gagacgggag aggagggcgt tcgctggact
     2401 gtgcgcgtgc ccagccaccg gttcgacatc gtgcgcgaag cggatctcgt cgaagaggtc
     2461 tgccgcatct acggttacaa caacgtgccg gttcgccagc cggtgaccga gctggagctc
     2521 acgccggtgc ccctcgaaag gagcccggca ggacggctga agcggctgct ggcaaccctc
     2581 ggctatcagg aagccatcac ctacagcttc gtggacccgc gtctgcagga tctgctcgcg
     2641 cccgccgccg atgccctggt gctgagcaat cccatgtcgt cggagcagtc ggtcatgcgg
     2701 ctgaacctgc tgcccggcct cgtcgaggcg ctgcgtttca acgtggcccg gcagcaggac
     2761 cgggtgcgga tattcgagct ggggtcgtgc tttgcccccg gcgaccccgt gctgcagaag
     2821 ctgatgcttg gcggtgtgct ctggggtcgg cggcacgggg agaactggaa ccagtcaggc
     2881 gcggaagtcg acttctacga tctcaagggc gacgtcgagg ccatcgtggg gtggagcggc
     2941 gagccggtgg cctacgagcg gctcgaagac ccggtgcttc accctggcca ggcggcgctc
     3001 gtggtgggtt cgcgagggcc ggtggggcgg ctggggcggc tgcatccgga gatcgaacgc
     3061 gcgctggatc tgccgggaca cgtcttcatc ttcgagctgg aggcagacgc ggttctgcat
     3121 cgcgcccgcc gcaggcatct gagcctgtcg aagttcccca gcgtcaggcg ggacctggcc
     3181 gtagtggttc aggccgatac ggcctcttcg agcatcgaga ggatcctccg cgagtccctg
     3241 ggcgaaattc tcgtcgactt caggatattt gacgtctatc agggcaaagg tattgattct
     3301 aatgagaaaa gccttggtat agggttgacg ttgcagcatc cgtcggccac ccttaacgac
     3361 caggaaattg gcaaatacat gaacgacgcc gtggacgccc tggttcgagg tgtgggcgca
     3421 aggctgcgtt gaagaatcgg acgcccgtcc ccgtgatctc aggggtcggg tgccgaaaac
     3481 acaggagact cccgggtggg tgcccttacc aaagcgcaga tggccgaccg cctcttcgat
     3541 gagctcgggc tgaacaagcg cgaagccaag gagatcgtcg agatgttttt cgacgaggtc
     3601 cgggactcgc tggagaacaa ccagcaggtc aagctctccg gattcgggaa cttcgatctg
     3661 cgtgagaagg gagagcggcc gggccgcaat ccgaaaactg gggaagagat ccccatcacg
     3721 gcccggcgcg tcgtgacgtt ccgacccggg cagaaactga aggcgcgagt ggaagcttat
     3781 gctggacccg ggtcagactg acgagctgcc gcccattccc ggaaagcggt acttcaccat
     3841 cggtgaggtg agccagctgt gcgaggtcaa gccccacgtg ctgcgctact gggagcagga
     3901 attcccccag ctcaagcccg tcaagcgtcg cggcaaccgc cgttactacc agcgtcagga
     3961 cgtcatgacc atccgccaga tccggtcgct gctctatgac caggggttta cgatcggggg
     4021 cgcccgacaa cagctggcgg gcgcagacgc caaacgagac cactcccagt tcaagctcct
     4081 ggtccggcag atgatcgtgg agctcgagga cctgctgagg gtcctcaagt cctgactccc
     4141 ggggcgcggc agcgatcttc catgcatgac gatgatggca cggcaggcgc aggcagggag
     4201 gaggggtccg gctccatacg ggtcgccatc gtgatcttcc tggttacctt tgtgctgctc
     4261 atggtcatgg ggatcatctg gatgttcatt cgcgtcgaag gctcgtcctc tggcggccaa
     4321 gctacggcca ccggcgaaaa cgcgtccgtc tgccgttgcc tggccgccgc ctccccagcg
     4381 cgcggcgagc ctcaggtcag cacgtttttc ggcacgtcgc gaaacgggcc cgtgtcaaag
     4441 cgcggctctt tcggcatgcc gaggacacgc tcggagatga tgttccgctg cacctcgtcc
     4501 gtgccgccgg cgatggacgt ggccggcacc gacaacagga tttccgacac cacgccgccg
     4561 agagggctct ccggaccggt gagcatggcg tcggtgccgc tgatcagcgt gtgaacgtgg
     4621 gaacacgcgc gcgccacgtt gctggcggcg agcttgccca gcgaaccttc cgggccctgg
     4681 ggccggccga gctgctgggc agcacgcgcc ctgcgtgccg tccactcggc gcttcgcgac
     4741 atgatcagcg cacgtgccat ggcctgccgc acgttaggct gcgccaaggc gcccgtggct
     4801 gatgcccgat cgatgactag atcgatgcga ccggcgcgct gggggtacca cttgtacggc
     4861 tccatcaccg tggcgagctc tgcccgctcc tcctcgtaga tgcggcctgg caggtctttc
     4921 gcgcgcgcgg cgtgacgcag cccgtcagcg ccgcgacgtt cgtgggcgag cgtggtcatg
     4981 gctacagccc agccgttgcc ggtcgtggat acctggaact ccggtgcgac cttggcgtcg
     5041 gtgatgaaga cctggttgaa ggacgcgtga ccgttcatct gcctgagagg ctgcacctcc
     5101 accccgggct gggacatgtc gaggacgaag taggacagcc cctgatgctt cggcacgtcc
     5161 cagtctgtgc gtgccagcag caatccccac tgggcgtgat gagcgctggt ggtccacacc
     5221 ttctggccgt tgatgatcca gctggtcccg tcgaagtcgg cgcgggtggt ggcaccggca
     5281 aggtccgacc cgctgccggg ctcgctgaag agctggcacc aggtgtcctc gccggtgagg
     5341 atccggcgca ggaacttctc cctgtgcatg tcgctgccgt gctcgagcag cgtggccgcc
     5401 gccagaagcc tgatgcccga ctttgccacg ctcaccgcgt tgacggacgc cagctcgtcg
     5461 tccacggccg gcgtcagccc caccggcagt ccccggccgt gccactgggt gggccaatgg
     5521 ggcatgcccc accccgagtc cacgaggatg ttgcgccact cgagcaggcc tagctgggga
     5581 tcccaattcg cttccagcca ggtcccgacc tcctgacgta cttccttttc gtcgatttcg
     5641 ctcatggtca acgtcctcag actccggtcc gctgcagata cagttcccgg tggtggtcgg
     5701 gggtgcccag aaagacctct gagctcttgg cccgtttgaa ccacaggtgc gtgtcgtgat
     5761 cccacgtgaa gccgatgccg ccgtggatct ggatgcagct tgccgccgtg ctcacgaacg
     5821 catcgcttgc cgcggctttg gcgaggcagg cgagggccgg caattcgggg tcgcccgaag
     5881 cggcggcctg ggctgcgtag taggcggcgg actttgccag ctccacttcc acgagcatgt
     5941 cggcgcaccg gtgcttgatg gcctggaagg atccgatggc gcggccgaac tgcatgcgga
     6001 ttttggaata ctccacggag ctgtccagca tgacctgggc tccgcccacg gattcgttgg
     6061 ccagggccac cgccgcgagg tccagcgttc gctgcagggc gggccagtcc gcgcctggct
     6121 ctcccagcgg ccgtgcgggc gcgtcgagca gatcgatgcg tgcgagcttg cgtgtggcat
     6181 ccatggtttc caacggggtc cgggtgaccc cggcatcgtc tggcctgacg agatacaacc
     6241 ctacgccggc ctcaccgccg gtgtccggtg tccgggccgc cacaatcagg aagccggccg
     6301 tcaggccatc gaccacgaag ctcttggtcc cggtcagcga gaagccggcg ccgctgtccc
     6361 gggccacggt cgcgatgccg tcgctgtccc atcgcccgcc ggtttcggtg accgccagcg
     6421 cgccgagttg ctcgcccgct gccagcgcgg gcaggagctc gcacttccaa tcttccgtag
     6481 cggcctcgag tatggcggtt gcggccagca cgctggagcc gaagtacggc gcgcacagga
     6541 gggaccgacc gagctcctcc atgatgatgc cgagctccac gggaccgaat ccggcgccgc
     6601 cgtactgctc cggaatgtga atacccggca gcgccagctc cccggacagc tgcttccata
     6661 ccgcctcgtc gaaaccctca gaggtctcca tcaggcgcct gacctcgggg gtcggggact
     6721 tgtcctgcag aaatcgccgg gtggcttccc ggaactgctc ct
//
LOCUS       DBNHQR010000281         3090 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1165428, whole genome shotgun sequence.
ACCESSION   DBNHQR010000281 DBNHQR010000000
VERSION     DBNHQR010000281.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1165428"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1238
                     /locus_tag="AC16VR_06405"
     CDS             <1..1238
                     /locus_tag="AC16VR_06405"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6010825.1"
                     /translation="EYIVGDAEPSLLIHEDVFSDEAEALKERCSVRALLETTASGTGN
                     AYEAAIDAAAPLTGSAEITHDDTWVIMYTSGTTGHPKGCMITYGMTFWNVVNLLNPHR
                     LSEDMVNLITLPLFHTGGLNVYGNPAVHMGGTNIVMRSFDPGETLRILQDAEYGITHM
                     IGVPANYLFMSQHPAFAEADLSRLRAMAVGGAPTSLELLKIYADKGNALQQAFGMTET
                     SPLVTALTPANALDKPGSAGLAVLHTEIRLVDEEAREITAPNVVGELWVKGPNVTPGY
                     WRRPDANESSFTDGWLHTGDAAYQDEDGFIYIVDRWKDMYISGGENVYPAEVENVIYQ
                     LPAVAEVAVIGVPHERWGETGQAVIVLKEGESLSEEAVLKHCKDKLARFKQPSSVRFI
                     DEIPHNATGKQLKRELREQ"
     gene            1314..1673
                     /locus_tag="AC16VR_06410"
     CDS             1314..1673
                     /locus_tag="AC16VR_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010826.1"
                     /translation="MAWHSAGCRVHRGAGATLYLQNLLNFDWEQTRSPAGAIIRLSTD
                     GSLRQSVPDAHVVGKFNPPVMTTADLALKFDPEYRKVAERFLADPDACQRQLKYTATP
                     VDLIFGSTAELRAIAAV"
     gene            complement(1764..2963)
                     /locus_tag="AC16VR_06415"
     CDS             complement(1764..2963)
                     /locus_tag="AC16VR_06415"
                     /inference="COORDINATES: protein motif:HMM:NF014954.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR6010827.1"
                     /translation="MNVQDDVPYTVEDLTAEWLSEGLKESGVLEGAAVTDFTMDVLGD
                     QVGFNGEVVIIAPTYSPPSASAPASLVLKIPTASKNRIMGQTMGLYEKEIRFYRDLRP
                     GLNIRTPRHYFSALDVADDPDVILERLRGMNRLPIWMIRGIVALANRFVSGNPRRYAL
                     LIEDLSGYRMGDQAAGCSDEDARHIVAAMARLHAQFWASETLDEKSWITPYAVTSRIM
                     QMRFLQGVDQYLCDEGGSLTPGRRDLIGWLRKNGIALTETFGDEPATLLHGDVRLDNI
                     CFDDATGDVVLFDWQTILAGPGASDLAYFISATLPGDADESQVDELIDVYHGELSQHG
                     VDVSRDRLRWQYEVGMLTMLHRVLPTTYPDQMELDAGRGLPLLQAWISRIYNRLEQID
                     HERILKI"
BASE COUNT          612 a          959 c          985 g          534 t
ORIGIN      
        1 tggagtacat cgttggcgac gccgagccga gcctgttgat ccacgaggat gttttcagcg
       61 acgaggccga agcgctcaag gagcgatgca gcgttcgcgc cttgctcgaa accaccgcaa
      121 gcggaaccgg caacgcttac gaggcggcga tcgacgcggc cgcaccgctg accgggagcg
      181 cggagatcac ccacgacgac acctgggtga tcatgtacac ctccggaacc accggccacc
      241 ccaagggctg catgatcact tacggcatga cgttctggaa cgtggtgaac ctgttgaacc
      301 cccatcggct gtcagaggac atggtgaacc tgataacgct gccgctgttt cacacggggg
      361 ggttgaacgt ctacggaaat ccggccgtgc acatgggagg caccaacatc gtgatgcgct
      421 cgttcgaccc gggcgagacc ctgcgcatcc tccaggatgc cgaatacggc attacccaca
      481 tgatcggcgt gcctgccaac tatctgttca tgagccagca tccggcattt gccgaagccg
      541 acctctcccg acttcgtgcc atggcggtag gcggggcgcc cacgtcactg gagctcctga
      601 agatctacgc cgacaagggc aacgcgttgc agcaggcgtt cggcatgacc gaaaccagcc
      661 ccctggtgac cgcgctgacg cccgccaacg cgctggacaa gccaggctcg gcagggcttg
      721 cggtgctgca taccgagatc cgtctggtcg acgaggaggc ccgggagatc accgcgccga
      781 acgtggtcgg ggagctgtgg gtgaaaggcc cgaacgtgac gcccggttac tggcgccgac
      841 cggacgcgaa cgaaagctcg ttcacagacg gctggctgca cacgggggat gcagcctatc
      901 aggacgagga tgggttcatc tacatcgtgg accgctggaa ggacatgtac atctcagggg
      961 gtgaaaacgt ctatccggcg gaggtggaga acgtcatcta ccagctgccg gcggtggccg
     1021 aggtggccgt gatcggggtg ccccacgagc gctggggcga gactggccag gccgtgatcg
     1081 tgctgaaaga gggagaaagc ctgtcggaag aagccgtgct gaagcactgc aaggacaagc
     1141 tggcacggtt caagcagccg agctcggtgc gcttcatcga cgagatcccg cacaacgcca
     1201 ccggcaagca gctcaaacgc gagctcagag agcaatgagc cctcggccag ccgcgttcaa
     1261 ctcagttctg gtggccggcc gagttgggta actaaggtcg gctgttcatc cgcatggcat
     1321 ggcacagcgc cgggtgccgc gtccatcggg gagcaggggc gacgctgtac ctgcagaacc
     1381 tgctgaactt cgactgggag cagacccgca gccctgcggg cgccatcatc cggctctcca
     1441 cggacggatc gctacgccag tccgtgccgg acgcccacgt cgtgggcaag ttcaacccgc
     1501 ccgtaatgac caccgccgat ctcgcgctga agttcgatcc ggagtacagg aaagtcgcgg
     1561 agcggttcct ggctgatccg gatgcgtgcc agcggcaact caagtacacc gccacgccag
     1621 tggatctgat cttcggctcc accgccgagc tgagggcaat tgccgcggtc taggcctacg
     1681 acgtcgggaa ggaacttttc ctgagcgact tcgtcgccgc ctgggtcaat ggcatgcggc
     1741 tggaccgctt cgacctgtag gcttcagatc ttaagaatac gctcgtggtc aatctgctcg
     1801 agccggttgt agatccgcga gatccaggcc tgcagcaacg gcaagccgcg cccggcatcg
     1861 agttccatct gatccggata ggtggtgggg agcacccgat gcagcatcgt cagcatgccc
     1921 acctcgtact gccagcgcag ccgatcccgt gagacgtcta ccccgtgttg cgaaagctca
     1981 ccgtggtaga cgtcgatgag ctcgtcgacc tgagattcat cggcgtcccc ggggagcgtc
     2041 gcgctgatga agtaggcaag gtctgaagcg cctggaccgg ccaggatcgt ctgccagtca
     2101 aacagcacga cgtcgccggt ggcatcatcg aaacagatgt tgtccaggcg aacgtctccg
     2161 tgcaggaggg tggctggttc atcaccgaac gtctccgtga gcgcgatgcc gttctttcga
     2221 agccagccga tgagatcccg gcggcccggc gtcagactcc cgccctcatc gcagaggtac
     2281 tgatcaacac cctgcagaaa acgcatctgc atgatcctgc tggtgacggc gtatggcgtg
     2341 atccagctct tttcatcgag cgtttcgctg gcccagaact gggcgtggag gcgagccatg
     2401 gctgcaacga tgtggcgagc atcctcgtca gagcaaccag cagcctgatc tcccatacga
     2461 tagccactca ggtcttcgat gagcagcgca tatcgacgcg ggttgccgct cacgaaccgg
     2521 ttggcgagcg cgacgatgcc gcggatcatc cagatcggca gccggttcat gccccgcaac
     2581 cgctcgagta tcacgtcggg atcgtcagcc acatccagcg cgctgaagta gtgacggggt
     2641 gtccggatgt tcagacccgg ccgcaggtcc cggtagaagc ggatctcctt ttcgtacagc
     2701 cccatggtct gccccatgat gcggttcttg gacgccgtag ggatcttgag gaccaggctg
     2761 gcgggagcgc ttgccgaggg cggcgagtaa gtcggcgcga tgatgacgac ctcgccgttg
     2821 aagcccacct ggtcaccgag cacgtccatg gtgaaatcgg tgacggcggc gccctcgagc
     2881 acgccggatt ccttgagccc ctcagacagc cactcggcgg tcaggtcctc gacggtgtag
     2941 ggcacgtcgt cttgtacgtt catcgttcgg atccggagcg gagatcgctt ttcggagtac
     3001 ggactgcagg ggctcgcgca tccggctccg aaatgtcaca tccatcattc tagcaaccag
     3061 aagtcacgca gggtcaacac gacgtccgcg
//
LOCUS       DBNHQR010000282         2016 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_596712, whole genome shotgun sequence.
ACCESSION   DBNHQR010000282 DBNHQR010000000
VERSION     DBNHQR010000282.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_596712"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..525
                     /locus_tag="AC16VR_06420"
     CDS             67..525
                     /locus_tag="AC16VR_06420"
                     /inference="COORDINATES: protein motif:HMM:NF041414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HIR6010828.1"
                     /translation="MRLQLALNVADIDEAVSYYSRILGVAANKREPGYANFVIEDPAL
                     KLVLFEAPGADERLNHLGFEAFADEDIEAVAGRLEDARVEHEVQTEEVCCFAKQNKVV
                     SYDPQGMMWEWYRVVEDSPTFFAEPEALACCAPAEPEAQGCCPPAERSAG"
     gene            complement(528..1322)
                     /locus_tag="AC16VR_06425"
     CDS             complement(528..1322)
                     /locus_tag="AC16VR_06425"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HIR6010829.1"
                     /translation="MPESLRVMVVCAHPDDAEFHAGGLLVQLAAAGTDIHLLTLTDGS
                     AGHAELDRASLARRRAREAQAAARRLGARCTVWDTPDGELEPSLQLRGRLIVAVREFA
                     PHVLITHRSGDYHPDHRAAAQLVQDASFLLRVPNVQPDAPIMNEDPVILGFCDFFRRP
                     SPFRSDLLVDTEEQLEAVVELLACHESQVYEWLPHIMGLEVGENRRSWLREFYTQRPA
                     AVAHAHADDGARYAEAFELSEYGRQVTPQELAGLLAAGSAKANHSG"
     gene            1489..1671
                     /locus_tag="AC16VR_06430"
     CDS             1489..1671
                     /locus_tag="AC16VR_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010830.1"
                     /translation="MYTVVDIASLQDYVLVEARRKAQEAGTVPIYRKFPVREVLDTLV
                     VDEEIVLFKPSLFPAL"
     gene            complement(1681..>2016)
                     /locus_tag="AC16VR_06435"
     CDS             complement(1681..>2016)
                     /locus_tag="AC16VR_06435"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HIR6010831.1"
                     /translation="QRVGAFFAHHDALLLPSAQVPPFDKSLDWVREIDGRAMETYIDW
                     MTICCAVTVTDLPAISVPGGFTDDGLPLGLQIVGRPHGDLDLLAIARLFEEATGHGQR
                     RPPQSEHAG"
BASE COUNT          343 a          607 c          699 g          367 t
ORIGIN      
        1 ggcgcgggtc aattatttcg aataaagtgg aaatatattt tcaaagtatt gatgcaggag
       61 aatcgcatgc gtcttcaact ggcactgaac gtcgcagaca tagacgaggc ggtgagctac
      121 tactcgcgga tcctgggcgt ggcggccaac aagcgggagc cgggctatgc caacttcgtg
      181 attgaagacc cggcgctcaa gctcgtgctc ttcgaagcgc ccggagccga cgagcggctg
      241 aatcacctgg gcttcgaggc ctttgccgac gaggacatcg aggcggtggc cggacggctc
      301 gaggatgcgc gcgtggagca cgaggtgcag accgaagagg tctgctgctt cgcaaagcag
      361 aacaaagtcg tcagctacga tccgcagggc atgatgtggg agtggtatcg ggtggtggag
      421 gactccccga ctttcttcgc ggagcccgag gccctggcgt gctgcgcgcc ggccgagccc
      481 gaagcccagg gctgctgccc ccctgccgag cggtcggcgg gttgaagtca gccgctgtgg
      541 ttggctttgg cagagcccgc ggcgagcagc ccggccagct cttgtggcgt gacctgtcgc
      601 ccgtactctg acagctcgaa agcctcggcg tagcgggcac cgtcgtcggc atgggcatgg
      661 gcgacggccg ccggccgctg tgtgtagaac tctctcagcc agctgcgacg gttctccccc
      721 acctcgaggc ccatgatgtg aggcaaccac tcgtaaacct gggattcgtg gcacgccagc
      781 agctccacca cggcttccag ctgctcttcc gtgtccacca gaaggtctga ccggaagggc
      841 gaggggcgac ggaagaagtc gcagaatccc aggattaccg gatcctcgtt catgatgggc
      901 gcatcgggct ggacgttcgg tacgcgcagc aggaagctgg cgtcctggac cagctgggct
      961 gctgctcggt ggtccgggtg gtaatcgccc gagcggtgcg tgatcagcac gtggggagcg
     1021 aattcgcgca cggcgacgat cagtcgcccg cgcagctgga ggctcggctc gagctcgccg
     1081 tctggcgtat cccagacggt gcaccgcgcg ccgagccggc gagcggcggc ctgggcttcc
     1141 ctggcccgtc gtcgggcgag cgaggcgcga tccagctcgg cgtgaccggc ggagccgtcc
     1201 gtgagggtga gcaggtggat gtccgtgccc gctgcggcca gctggacgag cagcccgccg
     1261 gcgtgaaact ccgcatcgtc gggatgggcg cagacgacca tgactcgtaa cgattccggc
     1321 atggcgtcag gtctccgtta catctcttac atccttttta catcaaccat ttacggcgtg
     1381 gatagactgt gggtgttggg ggttggaccc gcttcgggcc gttgttcgtt ggttttgaga
     1441 agcgactttc gctgtctcgt cggtcgcgat ttttgcgccg atgaggacgt gtacaccgtg
     1501 gtggacatcg cctcgttgca ggactacgtg ctcgtggagg ctcgccggaa agctcaggaa
     1561 gccggcaccg tccccatcta ccgcaaattt cccgtccggg aggttctgga cacgctcgtg
     1621 gtcgacgagg agatcgtcct cttcaagccg agcctgtttc ccgcgctcta gggtcgttat
     1681 tcagccggca tgctcgctct gcgggggacg ccgctggcca tggccggtag cctcctcgaa
     1741 cagccgggcg atggccagca gatcgaggtc gccgtgaggt cgcccgacga tctgcaggcc
     1801 caggggcagg ccgtcgtccg tgaacccgcc ggggactgag atcgcgggga gatcggtgac
     1861 ggtcacggcg cagcagatgg tcatccagtc gatgtacgtc tccatggccc ggccatcgat
     1921 ctcgcgcacc caatccagcg acttgtcgaa cggtggtacc tgggcagaag gcagcagcag
     1981 cgcgtcgtgg tgggcaaaga aagcgccgac gcgctg
//
LOCUS       DBNHQR010000283         1207 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_216328, whole genome shotgun sequence.
ACCESSION   DBNHQR010000283 DBNHQR010000000
VERSION     DBNHQR010000283.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_216328"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="AC16VR_06440"
     CDS             <1..216
                     /locus_tag="AC16VR_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010832.1"
                     /translation="SFGFTQFRIRHHGDVARLELLPDEIPAALDRAADIESAIRACGY
                     RFVAVDLRGFRSGSLNEGMVKVVNLPA"
     gene            290..1171
                     /locus_tag="AC16VR_06445"
     CDS             290..1171
                     /locus_tag="AC16VR_06445"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6010833.1"
                     /translation="MIEKIPFGRTGHESTRTLFGAAALGSMSPERAEGVLATLLEHGV
                     NHIDTAASYGDSELRLAPWLAANRASFFLATKTDQRTGSAARAQLERSLERLGVDQVD
                     LIQMHNLVDEQEWETAMAPGGALEALIDARAEGLVRFIGVTGHGTRVAAMHLKSLEQF
                     DFDSVLLPYNFTMMQQSDYAADVEALLKICKSRNVAVQTIKSVARRRWTDDDADKRFS
                     WYMPLRDRESLQRAVHFVLSRPGLFLNTSSDATILPLILEAASQPAEAPDPDAMRCDV
                     NALGMEPLFVRGVSDTI"
BASE COUNT          223 a          399 c          379 g          206 t
ORIGIN      
        1 tctttcggtt ttacgcagtt ccgtatccgg catcacgggg acgtggcgcg gctcgaactg
       61 ctgcccgacg agatacccgc cgcgctcgat cgtgcggcgg acatcgagtc ggccattcgc
      121 gcctgcggct accggttcgt tgccgtggac ctgcgaggtt tccggtccgg atctctgaac
      181 gaaggcatgg tcaaggtggt caacctgccc gcctgacccg gagggctcgt tgcaccgtcc
      241 tggaccgttg ggccatgatt acagtcactc gagcaagcag cggatcgaca tgatcgagaa
      301 aattcctttt ggccgcacgg gccacgagag caccaggacc ctattcggcg cggccgcgct
      361 gggaagcatg agcccggagc gcgccgaggg cgtcctggcc accctgctgg agcatggggt
      421 caatcacatc gacacggcgg caagctacgg cgattccgag ctgcggctgg cgccctggct
      481 ggcagcaaat cgggcgagct tctttctcgc caccaaaacc gaccagcgga cgggctccgc
      541 agcccgtgca cagctcgaaa ggtcgctgga gcgcttaggc gtggatcagg tcgatctcat
      601 tcagatgcac aatctggtcg acgagcagga gtgggaaacc gcaatggccc ccgggggcgc
      661 cctggaggca ctgatagacg ccagagccga aggcctggtc cgcttcatcg gcgtcaccgg
      721 tcacggtacc cgcgtcgccg cgatgcatct gaaaagcctc gagcagttcg acttcgactc
      781 cgtgctcctg ccctacaact tcacgatgat gcagcaatct gactacgcgg cagacgtgga
      841 agcgctgctg aagatctgca aatcccgaaa cgtcgcggtg cagacgatca agtcggtggc
      901 gcgccggcgg tggacggacg acgacgcgga caagcgtttc agctggtaca tgcccctgcg
      961 cgaccgggaa agccttcagc gagccgtgca cttcgtcctc tcccgccccg gcctcttcct
     1021 caacacctcc agcgacgcga ccatacttcc gctgatcctg gaggccgcga gccagcctgc
     1081 cgaggcgccg gatccggacg ccatgcgttg cgacgtgaac gccctaggga tggaaccgct
     1141 gttcgtccgc ggcgtctctg acacgatctg acgcaacaac cctgtgggcc agagcatcgc
     1201 catcgtc
//
LOCUS       DBNHQR010000284         5471 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1040319, whole genome shotgun sequence.
ACCESSION   DBNHQR010000284 DBNHQR010000000
VERSION     DBNHQR010000284.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5471)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5471)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5471
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1040319"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /locus_tag="AC16VR_06450"
     CDS             <1..1117
                     /locus_tag="AC16VR_06450"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6010834.1"
                     /translation="DPGDHYRYDIAGMSFLVVRTESGAIRTYPNACLHRGRMLKEFDG
                     HASELRCPFHGFAWHLDGSLKDVPAKWDFPHIDDDQFGLPEIPTAVWAGFVFINPDQQ
                     CAPFEDFISDMASQFERWDLGSLYKQAHVAKIMPANWKIAQEAFCEAYHVNGTHPQIL
                     RSLGDINSQVDVWENCARVITPSLTPSPLLEYDLTDGDVVRAMLDIPEGQPVPELPEG
                     MSPRAWSAAMARETLRPDAGDRVDEYCDAELVDNLDYTVFPNFHPWGAFNRIVYRFRP
                     NGNDHRSAIMECIMLAPFQGERPPPAKIRWLAEDEPWSSALGFLGRVFDQDAFNMPKV
                     QQGLEATYKPGITLANYQESKVRWLHHKLTEWVEDGE"
     gene            1114..1335
                     /locus_tag="AC16VR_06455"
     CDS             1114..1335
                     /locus_tag="AC16VR_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010835.1"
                     /translation="MTIIEHPFTGAIYRMNDEGNILVTQGDRQGVFRPDGRWVSGEIR
                     EADPQMCVWVSNVPASPGESDWHVTSDKT"
     gene            complement(1399..2226)
                     /locus_tag="AC16VR_06460"
     CDS             complement(1399..2226)
                     /locus_tag="AC16VR_06460"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6010836.1"
                     /translation="MSEKIVLVTGGAQGIGKGLARACLQRGASVVITNLDSEIAATTV
                     AELSEHGPIRAIRCDATDRNAVDALFDDVWATEGPVDLAFCNAGAGGMGQILETPMAD
                     VHHQFSTNLYSAIHLAQSYIPRLVEANRAGHVMFTGSENSLVLPRANADLAMGIYGGT
                     KHALLAVAEWLRYELRDTAVTVSLLMPGPVLTERLAATFDALADDPDNPEIRGRFPEH
                     VERTLRERFISTERCAEIALRGLAQGLFFIPTQAYIREDVDARYRELTAAFDALDLS"
     gene            complement(2255..2878)
                     /locus_tag="AC16VR_06465"
     CDS             complement(2255..2878)
                     /locus_tag="AC16VR_06465"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF036807.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family protein"
                     /protein_id="HIR6010837.1"
                     /translation="MALSVIGAGYGRTGTLSLKAALEQLGYLKCHHMIEVLQSPAQIE
                     GWMAAARGERVNWDELFAGYLASVDWPSCHFYRELADYYPQAKVVLTVRDPHAWYESM
                     NATTLRVIRRSMAEHPDRPSLGTELVVKGAFGGNIDSAAHAVEIFEAHTAEVMANIPS
                     ERLLVFDVREGWEPLCNFLQRDVPAAAFPRVNSREEFDGIFFGNRAD"
     gene            complement(2883..3689)
                     /locus_tag="AC16VR_06470"
     CDS             complement(2883..3689)
                     /locus_tag="AC16VR_06470"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6010838.1"
                     /translation="MATRHYDTIEVESEGQVDWLTLNRPESLNAINTPMVTELRDYFG
                     GLQENADTRIVVMRGAGRAFCAGLDIKQARSGEHDNPFGAGMGFQGYLAEVYIRMRRC
                     PQPIISLIQGPACGGGFAFVLASDIRIAGESARMNAAFIRIGLSACDMGVSYFLPRLV
                     GTSVASELMLTGRFIHAARALATGLVSEVVPDAELVDAARPYIDEMLTTSPMGLRLTK
                     EGLNMAVDAASLEAAMAIENRNQVLCSKTSDAREGMQAFLEKRAPEYTGA"
     gene            3783..4268
                     /locus_tag="AC16VR_06475"
     CDS             3783..4268
                     /locus_tag="AC16VR_06475"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6010839.1"
                     /translation="MIRGIHHVAVSTPDLDRIVRFYCDVMGAEQVYEGGWEKGSQLID
                     GIVGLKDSSCRQAMLKLGNAYLEFFEYQTPEPRPGDPARPVCDHGYTHFAIDVTDIDS
                     EYERLKAAGVKFNCPPPDFEGGPIRATYGRDPDGNVIEIQEIKDTAHEFYLDAWSGGR
                     S"
     gene            4342..4890
                     /locus_tag="AC16VR_06480"
     CDS             4342..4890
                     /locus_tag="AC16VR_06480"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6010840.1"
                     /translation="MAETAQTVPTLGVSSETMAAARQDIEYLRRWYAKATDLLGENTP
                     ESIAEGTKIYHRIFTADAYIRTSGATTQPFEATGPDAWAEVANTALESYVATQHLIGT
                     QIVDIHELTQDAGGTITSGRASMTSYLQAWHAGEENLWLFIGTYMDEVRFTPGVGWQI
                     HDMNLVQVSGEHRALGGPRPTP"
     gene            4962..>5471
                     /locus_tag="AC16VR_06485"
     CDS             4962..>5471
                     /locus_tag="AC16VR_06485"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR6010841.1"
                     /translation="MEILRSALIGGVLAVAVLMPEFAVAEGEGAMIEELVVTARRRAE
                     SVQDVPGTVTAIGQSTIEGAGVERVEDFIRLTPGVTLVNTAEVGDTQVNIRGINGARD
                     AENSFAFIVDGILHTNPAAFNREYPGLQQIEVFKGPQGAIYGRNAAAGAVIVTTERPT
                     EEFTGSIKTS"
BASE COUNT         1014 a         1673 c         1815 g          969 t
ORIGIN      
        1 cgaccccggc gatcactatc gttacgacat cgcaggaatg tcgtttctcg tggtccggac
       61 ggaaagcggc gccatcagga cgtatcccaa tgcctgcctg caccgggggc gcatgctcaa
      121 ggagttcgat ggccatgcct cggaactgcg ctgcccgttt cacggcttcg cctggcatct
      181 ggatgggtcg ctcaaggacg ttcccgcgaa atgggacttt ccccacatcg atgacgatca
      241 gttcggcctg ccggagatac cgacggcggt ctgggccggt ttcgtcttca tcaacccgga
      301 ccagcaatgc gcgccgttcg aggatttcat cagcgacatg gccagccagt tcgagcgctg
      361 ggacctgggc agcctttaca agcaggccca cgtggccaag atcatgccgg ctaactggaa
      421 gatcgcccag gaagctttct gcgaggcata ccacgtcaac ggaacgcatc cgcagatctt
      481 gaggagcctg ggcgacatca acagccaggt ggacgtctgg gagaactgcg cccgcgtcat
      541 cacgccctcc ctgactccca gccccttgct cgagtatgac ctgaccgacg gcgacgtggt
      601 gcgcgccatg ctcgatatcc cggaaggcca gcccgtcccg gagctgccgg aaggtatgtc
      661 gccgagggcc tggagcgcgg ccatggctcg ggagaccttg cgtccggacg caggcgacag
      721 ggtagacgag tactgcgatg cggagctggt agacaacctg gactacacgg tgtttcccaa
      781 ctttcacccg tggggcgcgt tcaaccgcat cgtctatcgc tttcggccga acggcaacga
      841 ccacaggtcg gccatcatgg agtgcatcat gctcgccccg ttccagggag aacgaccgcc
      901 tccggcgaag atccgctggc tggccgaaga cgagccctgg tcatcggcgc tcggctttct
      961 cggccgggtc ttcgatcagg acgcgttcaa catgcccaag gtgcagcagg gcctcgaggc
     1021 gacctacaag cccggcatca cccttgcgaa ctaccaggag tccaaggtgc gctggctgca
     1081 ccataagctc accgagtggg tggaggacgg cgagtgacca tcatcgagca cccgttcacc
     1141 ggtgccatct accggatgaa cgacgaaggt aacatcctgg tcacgcaagg cgatcgccag
     1201 ggtgttttcc ggcctgacgg acgatgggtc tccggggaga tccgcgaggc cgatccgcag
     1261 atgtgtgtct gggtgtccaa cgtgccggcc agccccgggg agtcggactg gcacgttacc
     1321 tcggacaaga cctgaccgcc ggagcaatcg ccgccgcgcc ccgtggcgca ggcgcatggc
     1381 gggtgagaag agggcggatc aggacagatc cagggcgtcg aacgcggcag tgagctcgcg
     1441 gtagcgtgcg tccacgtcct ctcgaatgta cgcctgggtc gggatgaaga agagtccctg
     1501 agcgaggccg cgcaaagcga tctccgcgca tctctcggtt gagatgaaac gctcccgcag
     1561 ggtgcgctcc acgtgctcgg gaaaccggcc ccggatctcc gggttgtccg ggtcgtcggc
     1621 cagggcgtcg aaggtggccg cgagccgttc ggtcagcacc ggccccggca tgagcaggct
     1681 gacggtgacg gccgtgtccc tcaactcgta gcgaagccat tccgcgacgg ccagcagggc
     1741 gtgcttggtc cctccatata tacccatggc cagatccgca ttggcccgcg gcaggacgag
     1801 ggagttctcc gacccggtga acatgacgtg gccggcgcgg ttcgcctcga ccaggcgtgg
     1861 gatgtagctc tgcgccaggt gaatggccga gtagagattg gtggagaact ggtggtgcac
     1921 gtccgccatg ggggtctcga gaatctggcc catgccccct gcgccggcgt tgcagaacgc
     1981 caggtcgacg gggccctcgg tggcccagac gtcgtcgaag agggcatcca cggcatttcg
     2041 gtcggtggca tcgcagcgaa tggcccgaat ggggccgtgc tcggacagct ccgctaccgt
     2101 cgtcgccgcg atctcggagt ccaggttcgt gatcaccacg ctcgcaccgc gttgcaggca
     2161 ggcgcgcgcc aggcctttgc cgataccctg ggcgccgccg gtgaccagga cgattttttc
     2221 gctcaacgat tccattttca tacctcggtt gcagtcaatc ggcgcgattg ccgaagaaaa
     2281 tgccgtcgaa ctcttcccgg gaattgaccc gtgggaaggc agcggcgggc acgtcgcgct
     2341 gcagaaagtt gcacagtggc tcccatcctt cgcggacgtc gaacaccagc agccgctcgg
     2401 agggaatgtt ggccatcacc tcggcggtgt gggcttcgaa gatctcgacg gcgtgggcgg
     2461 cgctgtcgat gtttccgccg aaagcgcctt tcacaaccag ctcggtgccc aggctggggc
     2521 ggtccgggtg ctcggccatc gaacgacgaa tgacccgtag cgtcgtcgcg ttcatgctct
     2581 cgtaccatgc atgggggtcg cgcacggtca gcaccacctt ggcctgcggg tagtagtcgg
     2641 cgagctcccg gtagaagtgg caggagggcc agtcgacgct ggccaggtag ccggcgaaga
     2701 gctcgtccca attgacccgc tcgccgcgcg ccgcggccat ccagccctca atctgcgccg
     2761 ggctttgcag cacctcgatc atgtggtggc acttcaggta cccgagctgt tccaaagcgg
     2821 ctttcagcga cagggttccc gtccggccgt aaccggcgcc gatgacagac agagccaccc
     2881 gcctaagcgc cggtgtactc aggcgcccgt ttctccagaa atgcctgcat accttccctg
     2941 gcgtcgctgg tcttcgagca gagcacctgg ttacggttct ctatggccat ggcggcttcc
     3001 aggctggctg catccaccgc catgttgagg ccctccttgg tcaggcgcag gcccatgggt
     3061 gatgtggtca gcatctcgtc gatgtacggc ctggccgcgt ccacgagctc cgcgtcgggg
     3121 accacctcgg ataccagccc cgtggccagc gcccttgcag cgtggatgaa ccggccggtc
     3181 agcatcaact ctgaggccac cgaggtgccg acgagtcgtg gcagaaagta gctcacgccc
     3241 atgtcgcagg ccgaaaggcc gatgcgaatg aaggcggcgt tcatgcgtgc gctctcgccg
     3301 gcgatgcgga tgtcagaggc cagcacgaaa gcaaagccgc cgccgcaggc cggcccctgg
     3361 atcagcgaga tgatgggctg ggggcagcgg cgcattcgga tgtagacctc ggccaggtaa
     3421 ccctggaagc ccatgccggc cccgaagggg ttgtcatgct cgccggatct ggcctgcttg
     3481 atgtccagcc cggcgcagaa ggcgcgcccg gcgccccgca tcaccacgat acgagtgtcg
     3541 gcgttctcct gaagaccgcc gaaataatcc cgcagctcag tcaccatggg cgtgtttatg
     3601 gcgttcagag actccggccg gttcagcgtc agccagtcga cctggccttc gctttcgacc
     3661 tcgatggtgt cgtagtgtcg tgtggccatg ctcgactccc tgctgatagg ccggtgcgga
     3721 tgttctccga cctcgccttt caatgttaat gtccgtgaat ctcgacttga aggggatgaa
     3781 aattgattcg aggcattcac cacgtcgccg tgtcgacccc agatctggac aggatcgttc
     3841 gtttctactg tgacgtcatg ggggcagagc aggtctatga ggggggttgg gaaaagggtt
     3901 cccagctcat cgacgggatc gtcggcttga aagactcgtc ctgccgtcag gccatgctga
     3961 agctgggcaa cgcctatctg gagttcttcg agtatcagac gcccgagccc cgaccgggag
     4021 atcctgcgag accggtttgc gatcacggct atacccattt cgcaatcgac gtcaccgaca
     4081 tcgattcgga gtacgagcgc ctcaaagcgg ccggggtcaa gttcaactgc ccgccccccg
     4141 atttcgaagg cggtccgatt cgcgcaacct acggacgcga tccggacggc aacgtcatcg
     4201 agattcagga aatcaaggat acggcgcacg agttctacct ggacgcctgg tcgggagggc
     4261 gaagttgaag gcgatgtcgt tgctagccaa gttgcgggcg ttcgcgctat tgtcgcggct
     4321 acgttgatgg gaggaagtgt gatggcggaa acggctcaga ccgtcccgac gctcggggtg
     4381 tccagcgaga cgatggcggc tgcgcggcag gacatcgagt acttgaggcg ctggtatgcc
     4441 aaggcaaccg acctgcttgg tgagaacacg ccggagtcaa tcgctgaagg caccaagatt
     4501 tatcaccgga ttttcacggc tgacgcctac attaggacgt cgggcgccac cacccagccg
     4561 ttcgaggcga cggggcctga tgcctgggcg gaagtggcca acaccgctct ggagagctat
     4621 gtggcaacgc agcacctgat cggcacgcag atcgtggaca tccacgagct gacgcaggat
     4681 gcgggcggga cgatcacgtc cggccgggcg tcgatgacca gctacctgca ggcgtggcat
     4741 gcgggtgagg agaatctgtg gctgttcatc ggaacctaca tggacgaagt tcggttcacg
     4801 ccgggcgtgg ggtggcagat acacgacatg aatctggttc aggtttcggg ggagcatcgt
     4861 gcgctcgggg ggccgcgtcc aacaccgtag gtcaggagcg gtgttcctga tcccgaatga
     4921 cgagggtcag aatcacaagg gtcaataggg ggtaagaaac gatggaaatc ttaagaagcg
     4981 cgttgatcgg cggggtgctg gcggtagccg tgctcatgcc tgagttcgct gtcgccgagg
     5041 gcgaaggggc gatgatcgag gagctggtgg tgactgcccg gcgccgggcg gagtccgttc
     5101 aggacgtgcc cgggacggta acggccatcg ggcagtcgac catcgaaggg gccggggtcg
     5161 agcgggtgga ggacttcatc cggctcactc ctggtgtgac gctggtaaat acggctgagg
     5221 tgggtgacac ccaggtcaac atccgcggta tcaacggcgc ccgcgacgcg gagaacagct
     5281 ttgcgttcat cgtggacggg atcctgcata ccaatccggc agcattcaac cgggagtacc
     5341 cgggcctgca gcagatcgaa gtgttcaaag ggccccaggg cgccatctac ggccgtaacg
     5401 ccgcggccgg ggcggtcatc gtcaccacgg agcggccgac cgaggagttt accggcagca
     5461 tcaagacgag c
//
LOCUS       DBNHQR010000285         2186 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_977422, whole genome shotgun sequence.
ACCESSION   DBNHQR010000285 DBNHQR010000000
VERSION     DBNHQR010000285.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_977422"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="AC16VR_06490"
     CDS             <1..948
                     /locus_tag="AC16VR_06490"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIR6010842.1"
                     /translation="EAGVPDVGEGQFLLRNAYVSLDAGFRNWMNEDSGDDVLPAMALN
                     APVMGLVLGQVVESRHSEYPVGEWLMARLAWEEYSISDGSDFISRLPEPRECPLPHYL
                     GILGDTGLSAYFGLLDIGRPQPGETVLVSAAGGAVGNVVGQLAKIHGAKAVGLAGTDE
                     KCARLVEELGFDAAVNHRSPDLAGVIAAACPDGVDVYFDSVGGPLLETVLWQIANGAR
                     IVMCGAVAAYNALEPIPGPVNLFQLVTHQALMQGYLTHLAEHRYPEARAAMTRWLKSG
                     ELKNVEYRLQGIENVGRAFCDLFGGNNFGKTIVRLRDEQ"
     gene            986..1222
                     /locus_tag="AC16VR_06495"
     CDS             986..1222
                     /locus_tag="AC16VR_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010843.1"
                     /translation="MPILKHPLSGAIYTARDDGLVEVDNNGLKGLFHFDGRHHSGELR
                     HADPHMLLWLAGPQLPADAAVRINRPAPVPPSEG"
     gene            1279..>2186
                     /locus_tag="AC16VR_06500"
     CDS             1279..>2186
                     /locus_tag="AC16VR_06500"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6010844.1"
                     /translation="MAAIVDRSKGISYQELIAQDPVAPPKTLTLENAYQPGIATRVPV
                     SRYASKEFHDLEMEKLWPRVWQMACREEEIPEVGNYTIYEIGSFSIVVVRTNQGIKAH
                     HNVCRHRGRRLCDHPGHASSFICPFHGFSWNIDGSLRSVTSEWDFPHIDKDELTLTPV
                     RCETWAGWVFVNMDMNAEPLMDFLGDLPAHFAVWEPENRYIEAHVAKIMSCNWKACQE
                     AFMEAFHVINTHPQILVGIGDENTQYDVWGNFSRAITPNGTPSPHLRFEPDEQELFDS
                     VTMRYMDRPSLGEVPEGMTARAMMAAG"
BASE COUNT          402 a          702 c          719 g          363 t
ORIGIN      
        1 gaggcagggg tacccgacgt cggggaaggg cagtttcttc tgcgcaatgc ctacgtctcc
       61 ctggatgcgg gtttccgcaa ctggatgaac gaggacagcg gcgacgacgt cctcccggcc
      121 atggcgctga acgcgcccgt catggggctc gtcctggggc aggtggtcga gtcacggcac
      181 tccgagtatc cggtaggcga gtggctcatg gccaggctgg cctgggagga gtactcgatc
      241 agcgacggct ccgacttcat ttcgcggctg ccggaacctc gcgagtgccc gctgccgcat
      301 tacctgggga ttctgggtga tacggggctc tcggcctatt tcggcttgct ggacatcggc
      361 cggccgcagc ccggtgagac ggtgctggtg agcgccgcgg gcggagccgt cggcaacgtg
      421 gtggggcagc tcgcgaagat tcacggggcc aaagccgtgg gcctggccgg taccgacgaa
      481 aagtgcgcgc gtctcgtcga agagctcggc ttcgacgcgg cggtcaatca ccgaagcccg
      541 gatctggccg gtgtcatcgc ggcggcctgt cccgacggtg tcgacgtcta cttcgacagc
      601 gtcggcggcc cgctgctgga gactgtgctc tggcagatcg ccaacggcgc caggatcgtc
      661 atgtgcggcg cggtggccgc ctacaacgcc ctggagccga tacctgggcc ggtcaacctc
      721 tttcagctgg tgacccatca ggcgctcatg cagggctacc tcacgcacct cgccgagcat
      781 cgctatccgg aagcccgggc cgccatgacg aggtggctga agtcgggcga gctcaagaac
      841 gtcgagtacc ggcttcaggg catcgagaac gtcggccgcg cgttctgcga tctgttcggg
      901 ggaaacaatt tcggcaagac catcgtgcgg ttgcgcgacg agcagtgatg cggtacctgt
      961 atacgcagag gaggagaaga agaccatgcc gattctgaag catcccctgt cgggagccat
     1021 ttacacggcc cgggacgacg ggctcgtgga ggtggacaac aacgggctca aaggcctgtt
     1081 tcacttcgac ggtcgccacc attccggcga gctgcgccac gccgatccgc acatgctgct
     1141 gtggctggcc gggccccagc tgccggctga cgcggccgtg cgcatcaacc gccccgcacc
     1201 ggtgccgccc tccgagggct gaaccggcgc accgggcgcg gacgacaccg aaccaaccga
     1261 catatccagg agaacgaaat ggccgccatc gtcgaccgat cgaaaggcat cagctaccag
     1321 gagctcatcg cgcaggatcc cgtggcgccg ccgaagaccc tgaccctcga gaacgcctac
     1381 cagccgggca tcgcgacccg cgtccccgtg agccgctacg catcgaagga atttcacgat
     1441 ctggagatgg agaagctgtg gccccgggtc tggcagatgg cctgccgtga ggaggagatc
     1501 cccgaggtcg gtaactacac catctacgag atcggcagct tctccatcgt ggtggtccgt
     1561 accaaccagg gcatcaaggc acatcacaac gtctgccgtc accggggccg acggctttgt
     1621 gatcatccgg ggcacgcctc gagcttcatc tgtcccttcc acgggttcag ctggaacatc
     1681 gacggatcgc tgcgatcggt cacctccgag tgggatttcc cccacataga caaggacgag
     1741 ctgacactca cgcccgtgcg ttgcgagacc tgggccggct gggtgttcgt caacatggac
     1801 atgaacgccg agccgctcat ggacttcctg ggcgatctgc ccgcgcactt cgccgtctgg
     1861 gagccggaga accggtacat cgaagcccat gtggccaaaa tcatgagctg caactggaaa
     1921 gcgtgtcagg aagccttcat ggaggccttc cacgtcatca atacccaccc gcagattctg
     1981 gtggggatcg gcgacgagaa cacccagtac gacgtctggg gcaacttctc ccgggccatc
     2041 acgcccaacg ggacgcccag cccgcacttg aggttcgagc cggacgagca ggagcttttc
     2101 gactcggtga ccatgcgtta catggatcgg ccctccctgg gggaggtccc cgagggaatg
     2161 accgcccggg ccatgatggc ggccgg
//
LOCUS       DBNHQR010000286         1815 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_788033, whole genome shotgun sequence.
ACCESSION   DBNHQR010000286 DBNHQR010000000
VERSION     DBNHQR010000286.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_788033"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="AC16VR_06505"
     CDS             complement(<1..224)
                     /locus_tag="AC16VR_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010845.1"
                     /translation="MSFTKVMAAVDWDEASAAVVIETARRAAGGRPVEIVHVLDQGYY
                     LNTIDPASASIEDLRAKASSEAEAYLQGLC"
     gene            420..725
                     /locus_tag="AC16VR_06510"
     CDS             420..725
                     /locus_tag="AC16VR_06510"
                     /inference="COORDINATES: protein motif:HMM:NF020317.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HIR6010846.1"
                     /translation="MATYIMLSTLTDEGRKTLKQRPERLKEVNKEVESMGARVVSQYA
                     VLGGYDFVNIIEAPTNEVMSRISMELGARGTIKVTTMPGMTIESFVDMLEGKTTSGN"
     gene            729..1727
                     /locus_tag="AC16VR_06515"
     CDS             729..1727
                     /locus_tag="AC16VR_06515"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR6010847.1"
                     /translation="MPRSVMLMRYHSAGMRAIESNPQTLYEIHDAIARWEAKVLESYR
                     LLGEWDQCTIFEAPDNFKAYRATLAQELGTTADTEILPAIDFDLFSRLVSTEAGSVGP
                     HRWQITWWARIGRRLFRWHAYDRFANRYCKPLTVTGLHKFDTIKGPCIIVANHTSHMD
                     ALVLNASLPNRIRWNVYSGAAADRWFVKGRKELVMQPWYQSLAMGTFPIQRGGGSKAL
                     DYPKWLLENGCNLIIFPEGTRSTSRSMAKFRHGVSVLALEKNVPVVPVFLTGLKAMRP
                     KGSREIFPGPAGANVLDPIYFEEGTTVPDATRRIYDALNEVHLKVGEFGDEAARQD"
BASE COUNT          360 a          570 c          545 g          340 t
ORIGIN      
        1 tcgcagagac cctgcagata agcctcggcc tcggaggagg ccttggcgcg gagatcttcg
       61 atggaggcgg aggcgggatc gatggtgttg aggtaatacc cctgatccag cacgtgaacg
      121 atctccaccg gccggccgcc ggctgctctc cgggcggtct cgatcactac cgcggcgctc
      181 gcttcgtccc agtcgacggc ggccattact ttggtaaagc tcatgtttcc atctgccccc
      241 agcatgtttc acgagaaccc gttcccgtgg cgaatcaggc ttcagccctg gcggcctcgg
      301 ccaatggccg ggggactgct gaaacctcaa accatgctaa acgcccgtcg gcaaaggcac
      361 aacgctggcg cgtgaccggt aaaggcgtat gctattcgca gcaatagcgg ggaatcatcg
      421 tggcgactta catcatgctc agcacgctga ccgacgaagg gcgcaagacc ttgaagcagc
      481 gccccgagcg cctgaaagag gtgaacaagg aagtggaatc catgggggcc cgggtggttt
      541 cgcagtatgc cgtgctcggg ggatacgatt tcgtcaacat catcgaggcc cccaccaacg
      601 aggtcatgtc gcgcatctcc atggagcttg gagcacgagg caccatcaag gtgaccacga
      661 tgcccggcat gacgatcgag tcgtttgtcg acatgctcga gggaaagacc accagcggca
      721 actgaaacat gcctcgatct gtgatgctca tgcgttatca ctcggccggc atgcgagcaa
      781 tcgagtcgaa cccccagacc ctgtacgaga ttcacgacgc catcgcgcgc tgggaagcca
      841 aggtgctgga aagctatcgg cttctgggtg agtgggatca gtgcacgatt ttcgaggcgc
      901 ccgacaactt caaggcgtat cgggccaccc ttgctcagga actcggcacc acggcagata
      961 cggaaattct gcccgccatc gacttcgacc tcttttcccg gctggtcagc acggaagccg
     1021 gcagcgtcgg gccccatcga tggcagatca cctggtgggc caggatcggc cgacgcctct
     1081 tccgctggca tgcctacgac cgttttgcca atcgctactg caaacccctg acggtcaccg
     1141 gccttcacaa gttcgacacc atcaaaggtc cgtgcatcat cgtggcgaac catacgagcc
     1201 acatggacgc gctggtactc aacgcctcac tgccgaatcg tattcgctgg aacgtctact
     1261 ccggagcggc ggccgatcgc tggttcgtga aaggccggaa ggagctggtg atgcagccct
     1321 ggtaccagtc tctggccatg ggcacgtttc cgatccagcg aggcggcggt tcgaaggcgc
     1381 tggactatcc caagtggctg ctcgaaaacg gctgtaacct catcatcttt cctgagggca
     1441 cgcgctccac cagccggagc atggccaagt ttcgccacgg ggtgtccgtt ctggcgctgg
     1501 agaaaaacgt gcccgtcgtc ccggtttttc tcaccggcct caaagccatg cggcccaagg
     1561 gctcccgcga gatttttccc ggtcctgcag gcgccaacgt gctcgatccc atctacttcg
     1621 aggaagggac cacggttccg gatgccaccc ggcgcattta cgacgccctc aacgaggtcc
     1681 atctgaaggt tggagagttc ggcgacgagg cggcgcgtca ggactgagcg gggcccatgc
     1741 gttcgttcag ccaggcgatc aggtcgtcga ccacctcgtg ccggttgacc tcgttgagca
     1801 tctcgtggcg cccct
//
LOCUS       DBNHQR010000287         2576 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_850676, whole genome shotgun sequence.
ACCESSION   DBNHQR010000287 DBNHQR010000000
VERSION     DBNHQR010000287.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_850676"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="AC16VR_06520"
     CDS             <1..205
                     /locus_tag="AC16VR_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010848.1"
                     /translation="FRAMTAADLMVANSQRLQVVVANPGDSYDKLARKVSIKSYPAET
                     LRVLNGDHPIGEPRAGDYIKIVQ"
     gene            complement(221..>2576)
                     /locus_tag="AC16VR_06525"
     CDS             complement(221..>2576)
                     /locus_tag="AC16VR_06525"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HIR6010849.1"
                     /translation="RQVKLETPLRVYSSDEKLIAEFGERRVIPLKLEEAPPLFVAALL
                     DTEDKRFYDHSGIDYISLLNDTVELALNREIRSGASTITMQLARNISFSLEQTFIRKF
                     KEMLLALQIEQELGKDEILELYMNVVPFGKRAYGAQAAALTYYGKRLDELNLAQLAML
                     AGIPQAPTAGNPINGPARAVRRRNVVLRRMLDQGSISQAAFDEAIAAPITASLHDRAL
                     DVGAPFPAEWVRAELINRYSDLYTGGYQVYTTIVADLQQTATRSLRAGLKSYDRRHGY
                     RGPEQSLGALTEEEYLQALREARSYADLDPAVIVSVDEQGADALLGDGSRGRIAMAEM
                     SWARRYLDVDRRGPPPERPSDVVQVGDVVRVEAIAEPAEAGEAESAGDDPEAEPAPAW
                     RLAQLPDIQGALVSMDPRTGAVLALEGGLDFAMNQYNHALQAARQPGSGFKPFVYSAA
                     LDRGVTPASIFMDAPLVFDDQNLESQYRPDNDNNRYNGPTRLREALYRSINLVSMRVL
                     LEVGAGSVMDHVRKFGFDTSTFPRNLQLAIGGGTMAVTPLQMATAYAVFANGGWQIEP
                     HIVDRVVSLDGETVYQASHPRVCDPCDQDPATDLDSEEPANLEDLLAEADDAPIKAER
                     VVDERNTYIMNDMLKDVIRVGTGRRARTLERSDIAGKTGTTNDAADTWFNGYSPDVVT
                     TVWVGFPDHRPLGATEYGSNTPLPIWIEFMSQALANKPERVPAQPAGIVTMKIDRDSG
                     ELAGSQQTDAIFELFLAEHTPQPPPSAAAGPSEPGTDLKPMDIF"
BASE COUNT          415 a          815 c          863 g          483 t
ORIGIN      
        1 gtttcgcgcg atgaccgcgg cagatctgat ggtggccaac agccagcgtc tgcaggtcgt
       61 ggtcgccaac ccgggtgaca gctacgacaa gctcgcccgc aaggtgtcca ttaaatccta
      121 tcccgcagaa accctgcgcg tgttgaacgg cgaccacccc atcggggagc caagggccgg
      181 cgactacatc aagatcgttc agtagccctg caggctcggc tcagaagatg tccatcggct
      241 tgaggtcagt gccgggttcg ctgggtccgg cagccgccga tggcggtggc tggggcgtgt
      301 gctccgcaag aaacagctcg aatatcgcat ccgtctgctg ggagccggcg agctcgccgc
      361 tgtccctgtc gattttcatc gtgacgatcc cggccggctg agcgggaacc cgttccggct
      421 tgttggccag tgcctgggac atgaactcga tccagatcgg caggggcgtg ttggatccgt
      481 actccgttgc gcccagcggc cggtggtcgg ggaaacccac ccacacggtc gtcacgacgt
      541 cggggctgta accgttgaac caggtgtcgg cggcgtcgtt ggtggtgccg gtcttgcctg
      601 cgatgtcgct tcgctcgaga gtacgcgccc ggcgaccggt cccgacccgg atcacgtcct
      661 tgagcatgtc gttcatgatg taggtattcc gctcgtcgac tacccgctcg gccttaattg
      721 gcgcatcgtc ggcctcggcg agaagatcct cgaggttcgc gggctcctcg ctgtcgaggt
      781 ccgtagcggg atcctgatcg cagggatcgc aaaccctcgg gtggctggcc tggtagacgg
      841 tttcaccatc cagcgagacc acccgatcga cgatgtgcgg ctcgatctgc cagccaccgt
      901 tggcaaagac agcataggcc gtggccatct gcagcggcgt gaccgccatg gttccgccgc
      961 cgatggcaag ctgaaggttg cgcggaaacg tgctcgtgtc gaagccgaat ttgcgcacgt
     1021 gatccatgac gctgccggcg ccgacctcga gcagcacgcg cattgagacc aggttgatgg
     1081 atcggtagag ggcttcccgg agcctggtcg ggccgttgta gcgattgttg tcgttgtcgg
     1141 gccggtactg cgactccaga ttctgatcgt cgaacactaa gggcgcgtcc atgaatatgc
     1201 tcgccggcgt gaccccgcgg tcgagcgcgg ccgagtagac gaagggcttg aatccggagc
     1261 ccggctgccg ggcagcctga agcgcgtgat tgtactgatt catcgcgaag tccaggccgc
     1321 cttccagagc taaaacagcg ccggtacggg ggtccatgga caccagagcg ccctgaatgt
     1381 caggcagctg ggccagccgc caagccggcg ccggttcggc ttcgggatcg tctccggcac
     1441 tttccgcctc tccagcctcg gccggttcgg caatagcttc cacccgcacc acgtcgccca
     1501 cctggaccac gtccgagggc cgctcgggcg gggggccgcg gcgatccacg tccaggtagc
     1561 gtcgcgccca tgacatctcg gccatggcga tgcggccgcg gctgccgtcg ccgagcaggg
     1621 cgtccgcgcc ctgctcgtcc acggagacga tgacggccgg atcgaggtcg gcgtaggatc
     1681 tcgcctcgcg cagcgcttgg aggtattcct cttcggtgag cgcgcccagt gactgctcag
     1741 ggcctcggta gccgtggcgc ctgtcgtagc tcttcaggcc agcgcgcagg gaacgggtgg
     1801 ccgtctgctg cagatcggcc acgatggtgg tgtagacctg gtacccgccc gtatataggt
     1861 cgctgtagcg attgatcagc tccgcgcgga cccactccgc cgggaacggc gcgcccacgt
     1921 ccagggcgcg atcgtggagg ctggccgtga tcggggcggc gattgcttcg tcgaaagctg
     1981 cctgacttat cgagccctga tcgagcatac gccgcagcac cacgttgcga cggcgcaccg
     2041 cgcgcgccgg cccgttgatg ggattacccg ccgttggggc ctggggaatg cctgccagca
     2101 tggcgagctg ggcgagattc agctcgtcca gacgcttgcc gtagtaggtc agggcggccg
     2161 cctgcgcgcc gtaggcccgc ttgccgaacg gcaccacgtt catatacagc tcgaggatct
     2221 cgtccttgcc gagctcctgc tcgatctgca gcgcgagcag catctctttg aatttgcgga
     2281 tgaacgtctg ctccagggag aaggagatgt tgcgcgcgag ctgcatggtg atggtgcttg
     2341 cgcccgatcg gatctcccgg ttcagcgcga gctccacggt gtcgttgagc agcgagatgt
     2401 agtcgatgcc gctgtggtcg taaaaacgct tgtcttcggt gtccagaagg gcggcgacga
     2461 acagcggcgg cgcttcctcc agcttcaggg gtatgacccg ccgctcgccg aattccgcaa
     2521 ttagcttttc gtcgctcgag tagacgcgca gcggagtctc gagcttgacc tgccga
//
LOCUS       DBNHQR010000288         3271 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_850993, whole genome shotgun sequence.
ACCESSION   DBNHQR010000288 DBNHQR010000000
VERSION     DBNHQR010000288.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_850993"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="AC16VR_06530"
     CDS             complement(<1..896)
                     /locus_tag="AC16VR_06530"
                     /inference="COORDINATES: protein motif:HMM:NF024793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExeA family protein"
                     /protein_id="HIR6010850.1"
                     /translation="MYQDYFQLNQAPFSIAPDPNFLYLSAGHSEALAHLMYGFSHGGF
                     VLITGEVGTGKTTLLRNLIKQTPPDLDVAFILNPRLTVKELLETLCDELGVRYPEGSN
                     PTVKQYIDVLNRHLLAAHEKGRSTVVIIDEAQNLSPAVLEQIRLLTNLETDERKLLRI
                     ILLGQPELGALLDRAELRQLAQRITARYHLGALNREDTYAYIVHRLARAGGNPKLFSR
                     GALSRLFRISKGVPRLINVVADRALLGAYVEGRHQVTGRIVSRAAREVLGRRPRYRLW
                     IGVGAASVSAAGVAWAYLTLPEV"
     gene            complement(896..1777)
                     /locus_tag="AC16VR_06535"
     CDS             complement(896..1777)
                     /locus_tag="AC16VR_06535"
                     /inference="COORDINATES: protein motif:HMM:NF010621.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HIR6010851.1"
                     /translation="MAFLKGILATTLVALNTIVLCIPLFLVSAVRALSPAAIKRWLSR
                     KLDRWIIDGWVATNRACFGWLDLCDVEVQWQGAERLDRDRWYLVISNHQSWTDILLLQ
                     TSLYGHIPPIKFFTKRELIWIPFIGLAMRLLGFPFVRRMTREQIAKKPELVRADRDAT
                     LAACQEFRNHPTTVLNFLEGTRFTPAKQARQDARFKHLLNPKIGGLSYVLSGLSKELD
                     RLVDVTITYPRGTPTFWEFMQGKCPAAHLLVQCREIPQDVRAPGNDDEQREVVSPWIE
                     ALWREKDARLGAQTVAL"
     gene            1871..2899
                     /locus_tag="AC16VR_06540"
                     /pseudo
     CDS             1871..2899
                     /locus_tag="AC16VR_06540"
                     /inference="COORDINATES: protein motif:HMM:NF021535.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(2921..>3271)
                     /locus_tag="AC16VR_06545"
     CDS             complement(2921..>3271)
                     /locus_tag="AC16VR_06545"
                     /inference="COORDINATES: protein motif:HMM:NF013132.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HIR6010852.1"
                     /translation="GALLSGLAALHVLLIMLTICLTVTFLTETTSNTATTTLLMPILA
                     AGAVGAGISPELFMIPAAMSASCAFMLPVATAPNVIMFSTGRVAIETMVKEGFTLNLI
                     GAIVIATLCYVLLT"
BASE COUNT          553 a          997 c         1112 g          609 t
ORIGIN      
        1 acctcgggca gggtgaggta ggcccaggcg acgccggcgg ccgagaccga ggcggcaccg
       61 acgccgatcc agaggcgata gcgcggccgg cggccgagca cctccctggc cgcgcggctg
      121 acgattcgtc cggtgacctg gtgtctgccc tccacgtagg cgccgagcag cgcccggtcg
      181 gccaccacgt tgatcagcct gggaacgccc ttcgagatcc tgaacaggcg tgaaagcgcg
      241 ccccgggaaa acagcttcgg gtttccgccc gcgcgggcca ggcggtgcac tatgtacgcg
      301 taggtgtcct cccggttgag cgcccccagg tgatagcgtg cggtgatgcg ctgggcgagc
      361 tggcgaagct cggcccggtc cagcagggcg ccgagttcgg gctggcccag caggatgatt
      421 cgcagcagct ttctctcgtc cgtctccaga ttggtcagca ggcggatctg ttccagcacc
      481 gccggtgaga ggttctgggc ttcgtcgatg attaccaccg tgctgcgccc cttctcgtgg
      541 gccgccagaa ggtgccggtt gagcacgtcg atgtattgct tgaccgtcgg gttcgatccc
      601 tccggatagc gcaccccgag ctcgtcgcaa agggtttcca gcagctcctt gaccgtcaga
      661 cgcgggttga gaatgaacgc gacgtctaga tccgggggcg tctgcttgat caggttgcgc
      721 agcagcgtcg tcttgccggt tcccacctcg cccgtgatca gcacgaagcc gccgtgcgag
      781 aagccgtaca tcaggtgggc gagcgcctcg ctgtggcccg cgctcagata cagaaaattg
      841 ggatccggcg cgatggaaaa gggcgcctgg ttgagctgaa agtaatcctg gtacatcaca
      901 gcgcgaccgt ctgggccccc aggcgcgcgt ccttttcgcg ccacaaggcc tctatccagg
      961 gcgaaacgac ctcacgctgc tcgtcgtcgt tcccgggcgc cctcacgtcc tgtgggatct
     1021 cccggcattg caccagcagg tgcgctgccg ggcacttgcc ctgcatgaac tcccagaacg
     1081 tgggggttcc tcgggggtag gtgatggtga cgtcgacgag cctgtccagc tccttgctca
     1141 ggccgctcag gacgtaggac aacccgccga ttttcgggtt gaggaggtgc ttgaagcgag
     1201 cgtcctgccg ggcctgtttg gcgggtgtga agcgcgtgcc ctccaggaaa ttcagaaccg
     1261 tggtggggtg gttgcggaac tcctgacatg cggccagggt ggcgtcgcga tctgcgcgca
     1321 cgagctctgg cttcttcgca atctgttccc tcgtcatgcg ccgcacgaac ggaaatccga
     1381 gcagccgcat ggccagaccg atgaacggga tccagatgag ctcgcgtttg gtgaagaact
     1441 tgatgggtgg tatgtgtcca tacagtgatg tctgcagcag gagtatgtcg gtccagctct
     1501 gatggttgct gatcaccaga taccacctgt cgcggtcgag ccgttcggcg ccctgccatt
     1561 gcacctcgac gtcgcacagg tccagccagc cgaagcaggc gcgattggta gcgacccagc
     1621 cgtcgatgat ccagcgatcg agcttccggc tgagccaacg tttgatggcc gccgggctca
     1681 gcgcacgcac ggctgaaacc aggaacagcg ggatgcacag cacgatggtg ttcaacgcca
     1741 ccagcgtcgt tgccagtatg cctttcagga acgccacgaa accctcttat catacgtgct
     1801 gacctagcgc tactgcctgg gggatcccgg cttgagaatg cagacgacca cagcaggcgt
     1861 acggccgcat atgacgatcg ggcgcctgct ggcggcggcc gcggccgcct cgtttctctc
     1921 cggctgcgcg agcctcggct actacaccca ggccatgagc ggtcagctgc agatctcgtc
     1981 ttccgggcag cccgtgggcg aattgcttgc ccgtctcgaa gccgttgagg accccgatgc
     2041 gcttcaggcc ggtctcctct cgggcctgcg ctccagccag tcggtgctcg ctttcgcaga
     2101 agccgaactc gggctcgaag caggcggtag ctactcctcc tacgtcgagc tggatcggcc
     2161 ttacgtggtc tggaacgttt tcgcggctcc ggagctcagc ctcgaggcgc ggtcctggtg
     2221 ttatctcgtc gtcggctgcg ctccgtaccg tggctacttc gagcagggcc gtgccgagcg
     2281 gtttgcggca ggtctcaagg acaggggcct ggacgtctac gtggggggcg tgcccgcgta
     2341 ctccacgctc ggctggttcg aagacccgct gctgagcacg ttcattcgct ggccccaagg
     2401 aaggctggcc gagttgctga ttcacgagct ggctcactcc agggtctggg tggcgggtga
     2461 cgtggggttc aacgaagcgt ttgcgagctt cgtgggccga gagggcgccc ggagctggat
     2521 cgagcagcgc catggggccc aagcggccga tgagtttctc gagcgcggca ggagctggcc
     2581 ggcactgatg cggcttctgc tcgagatccg gcgggctctg gaggagcttt acatcagcgg
     2641 tctgagcgaa ggggaaaaac gggcgggcaa gtcgaaggtg atcgatcgtg ggaaagcctg
     2701 ttaccagagg aacatttccc gatacggatc cggaaggtac gacgaggttc tggccgggct
     2761 gaacaacgcg tttctcgttt ccctgtcgac ttacgacgac gacgttccgg ccttcgccgc
     2821 cgtcttcgcc caggtgtccg gagattggcg acgcttcttc gacgaggtcg agacgatggc
     2881 ccgcatgacc tccgagcgca gggccgatgc gatggaagcc ttacgtgagc agcacatagc
     2941 agagcgtggc gatgacgatg gcgccgatga ggttgagtgt gaagccctct ttcaccatgg
     3001 tctcgatggc gacacggccg gtgctgaaca tgatcacgtt gggcgcggtg gccacgggta
     3061 gcatgaaggc gcaggatgcg ctcatcgcgg caggaatcat gaagagctcg ggcgatatgc
     3121 ccgcgcccac ggcgcctgcg gcgagaatcg gcatcagcag ggtcgtcgtc gccgtgttgc
     3181 tcgtggtttc ggtgaggaag gtaacggtga ggcagatggt gagcatgatc agcagcacat
     3241 gcagcgctgc caggccggac agcagcgcgc c
//
LOCUS       DBNHQR010000289         3020 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_470092, whole genome shotgun sequence.
ACCESSION   DBNHQR010000289 DBNHQR010000000
VERSION     DBNHQR010000289.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_470092"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="AC16VR_06550"
     CDS             <1..455
                     /locus_tag="AC16VR_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010853.1"
                     /translation="DPAVWEEISRRAQPPRSWTLRYPLATAATVFLAAALTIVAWNPL
                     GNGGAGAGPEPVVVADGALVDLMSRSQALESHLPVQGTPAGQLAWGSAEEALRYRIAD
                     IDAQLMRIYETEPVDVERREALWRQRVDLLESLTQVQRGQAVVRPAIY"
     gene            465..1556
                     /locus_tag="AC16VR_06555"
     CDS             465..1556
                     /locus_tag="AC16VR_06555"
                     /inference="COORDINATES: protein motif:HMM:NF024578.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HIR6010854.1"
                     /translation="MIKWLSSRKRQVLAGFTAMALLLSGYTFADELEDRLREAREQLD
                     AAAERLAELHRQMYEAEVSEGRYGKRAMIGILLTSMGDDDGLVIAGVTPDGGAKQAGL
                     AAGDRIVAIGGVRLDTGGADKPGKLLSNVVKDLEPGDPVKVEYVRDGETLKADVVTQP
                     KRAFVMKSLAMELDQLDPEFTAELIKEFEDLGELKELKELGKLEALHELGDLGELIGN
                     VVDVGTSLKLEDMDGDLARYFGVDAGVLVVAPPSQEGELKSGDVLLELDGKDVANARE
                     AMKTLAMSDGTIDAGVLRNGKKREVSIDCSQFSAGDHMAITIDPSIIRIRRGIDGDDG
                     VDIRIVVDEEVVVDEKGSKKAAADDSAGG"
     gene            complement(1595..>3020)
                     /locus_tag="AC16VR_06560"
     CDS             complement(1595..>3020)
                     /locus_tag="AC16VR_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR6010855.1"
                     /translation="GMRIRLNLAQALMAPADLLLLDEPTNHLDLETTVWLESWLQRFA
                     GTLLVIAHDRAFLDGVCDHIVHIEHGRANSYRGNYSSFERQRAEALAQQQAAHARQQA
                     EIKHMQHFIDRFRAKASKAKQAQSRMRALERMQAVAPVHADSPYRFSFANPEQMSHPL
                     LSLEDVSVGYEGRAVIEHVRQTLLPGARIGILGANGAGKSTLLKCLVGELEPLAGTLV
                     RGRHSAVGYFAQHQLERLDGQTSALATLTNERPQAREQWCRDYLGRWGFPADMVERPI
                     VTLSGGEKARLVLALIALAQPAILVLDEPTNHLDLEMREALALALQDYAGALLLVSHD
                     RSLLRRAVDEFWLVEGGRLASFAGDIDAYAESRLRRSEPPSSERAPETNRKALRQAAA
                     AQREAAKPLRAKLRHLEKEMDQIGVELREVESRLADGDVYRSLPPEELDDLLSAAGRL
                     RKRLDATEQAWLSATEALEALEQS"
BASE COUNT          504 a          969 c         1044 g          503 t
ORIGIN      
        1 ccgatccggc ggtgtgggag gagatcagcc gcagggcgca accgccgcgc agctggacgc
       61 tgcgctaccc cctggcgacc gcggcaacgg tctttctggc ggcggccctc accatcgtcg
      121 cctggaaccc ccttggcaac ggcggagccg gcgccggtcc agagcccgtc gtggtggccg
      181 atggtgccct ggttgacctg atgagccgat cccaggctct cgagtcgcac ctgcccgtgc
      241 agggcacgcc cgccggtcag ctggcctggg gctctgccga ggaggcgctc aggtaccgga
      301 ttgccgatat agatgcgcag ctcatgcgga tctacgagac cgaaccggtg gacgtcgagc
      361 gtcgtgaagc gctgtggcgg cagagggtcg acctgctcga atcgcttact caggtgcagc
      421 gcggccaggc cgtggtgcga cctgcaattt actaggagtc gatgatgatc aagtggttga
      481 gctcgcggaa gcggcaggtg ctcgccgggt tcacggcgat ggcactgctg ctctccggat
      541 atacctttgc cgacgagctg gaggaccggc tgcgcgaggc ccgcgaacag ctcgatgccg
      601 ccgccgagcg gctcgccgag ctgcatcggc agatgtatga agccgaggtt tcagagggtc
      661 gctacgggaa acgcgcgatg atcggtatcc ttctgacgag catgggcgac gacgacggcc
      721 tggtgattgc tggcgtgacg cccgatggcg gcgcgaagca ggctggactg gccgcgggcg
      781 atcggatcgt cgccatcggc ggcgtgcggc tggacaccgg cggtgcggac aaacccggca
      841 agctcctcag caacgtggtc aaggacctcg agcccggcga ccccgtgaag gtggaatacg
      901 tgcgcgacgg cgagacgctg aaggccgacg tggtcacgca gcccaaacgt gccttcgtga
      961 tgaagtccct ggcgatggag ctggatcagc tcgatcccga attcacggcc gagctcatca
     1021 aagagttcga ggacctcgga gagctcaagg aactcaaaga actcggcaag ctggaggctc
     1081 ttcacgagct cggagatctg ggcgagctga tcggcaacgt cgtcgacgtg ggcacctcct
     1141 tgaagctcga agacatggac ggcgacctgg ccaggtattt cggagtcgac gcaggcgtcc
     1201 tcgtggtcgc tccgccttca caggaaggcg agctcaagag tggggacgtg ctgctcgagc
     1261 tcgacggcaa ggacgtggcc aacgcccggg aagccatgaa gacgctcgcc atgagtgacg
     1321 ggaccatcga cgccggggtt ctgcgcaacg gcaagaaacg cgaggtgtcc atcgactgct
     1381 cgcagttctc cgcgggcgat cacatggcca tcacgatcga tccgagcatc atccggattc
     1441 ggcgtggcat cgacggcgac gatggagtcg acattcggat cgtcgtggac gaggaggtgg
     1501 tcgtggacga gaaaggctct aaaaaagcag ccgccgacga ttcggcgggc ggctaggcgt
     1561 ccggaccgac cctcatcccc tccgggcgcg ccgttcagct ctgctcgagc gcttcgagcg
     1621 cctcggttgc gctgagccag gcctgctcgg tcgcgtccaa tctttttctc agcctgccgg
     1681 cagcggagag caggtcgtcg agctcctccg gaggcaggct ccggtacacg tccccatccg
     1741 ccagtcggga ctccacctcg cgcagctcca cgccgatctg atccatctcc ttttccaggt
     1801 gcctgagctt tgcccgcaac ggcttggccg cctcccgctg ggcggccgcc gcctgtcgca
     1861 gggctttgcg gttcgtctcg ggagcgcgtt cggatgacgg aggttcgctt cgcctcagac
     1921 ggctctcggc gtaggcgtcg atatcgccgg cgaaactcgc aagcctgccc ccctcaacca
     1981 gccagaactc gtccacggcc cgccgcagca ggctgcgatc gtgggagacc agcagcaggg
     2041 cgcccgcata gtcctgcaac gccagggcca gggcctcgcg catttccagg tccaggtggt
     2101 tggtcggctc gtcgaggacg agaatggccg gctgggcgag agcgatcagg gccagcacga
     2161 gacgggcttt ctcgccgccg gaaagcgtaa cgatgggccg ctccaccatg tcggcgggga
     2221 agccccagcg gcccaggtag tcccggcacc attgctcccg ggcctgtggc cgctcgttcg
     2281 tgagggtcgc cagagcggac gtctgcccgt ccagccgttc gagctgatgc tgggcgaagt
     2341 acccgaccgc ggagtggcgt ccgcgcacca gcgtgcccgc caggggctcg agctcgccca
     2401 ccagacactt gagcagggtg gacttgccgg cgccgttggc tcccagtatg ccgatgcggg
     2461 cgccgggcag aagcgtctgc ctgacgtgct cgatcaccgc gcgtccttcg tagcccacgg
     2521 acacgtcctc caggctcagc agggggtggg acatctgctc cggattcgcg aagctgaagc
     2581 ggtagggcga gtcggcatgc acgggggcta cggcctgcat gcgctccagg gccctcattc
     2641 ggctctgtgc ctgcttcgct ttgctggcct tggcgcgaaa ccggtcgatg aaatgctgca
     2701 tgtgcttgat ctcggcctgc tgcctggcgt gggccgcctg ctgctgcgcc agcgcttccg
     2761 ctcgttgccg ctcgaacgag gagtagttgc cccggtagga gttcgcgcgc ccgtgttcga
     2821 tgtgaacgat gtggtcgcac acgccgtcga ggaaggcccg atcgtgcgcg atcacgagca
     2881 gggttccagc aaagcgctgc agccaggatt ccagccagac cgtggtctcc aggtccaggt
     2941 ggttggtagg ctcgtccaga agcagcagat cggccggagc catcagcgcc tgggccagat
     3001 tcaggcggat ccgcatgccg
//
LOCUS       DBNHQR010000290         1762 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_597688, whole genome shotgun sequence.
ACCESSION   DBNHQR010000290 DBNHQR010000000
VERSION     DBNHQR010000290.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_597688"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="AC16VR_06565"
     CDS             complement(<1..281)
                     /locus_tag="AC16VR_06565"
                     /inference="COORDINATES: protein motif:HMM:NF016158.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HIR6010856.1"
                     /translation="MSKVFLSFGNQLFHPRYLREHADAEFVMVEDEQFCRRYAFHKQK
                     LTFVLSAMRSYAQTLRGQGWRVRYFDLDSAATWRSAVREAVAATSADTL"
     gene            complement(306..1028)
                     /locus_tag="AC16VR_06570"
     CDS             complement(306..1028)
                     /locus_tag="AC16VR_06570"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6010857.1"
                     /translation="MSRKVAVVAGAAGGLGEALSRALVAHPDYASVFALSRTRPSSDD
                     GIEWFETDTTSPVDLESAASRVAQLTDRVHLLLCCSGVLHGGAGSPHRPEKSLAELEL
                     ENLQLAVRVNAFAPLAAIRAFRDLLRHDQRPVAAVLSAMVGSIGDNSLGGWYSYRMSK
                     AALNMGVRNVAIEFGRLRNGPSVVAVHPGTTLTPLSEPFVRGRDPRTPDESARHILSV
                     VHDLSPEDSGRFFNWDGRELPW"
     gene            complement(1209..>1762)
                     /locus_tag="AC16VR_06575"
     CDS             complement(1209..>1762)
                     /locus_tag="AC16VR_06575"
                     /inference="COORDINATES: protein motif:HMM:NF021623.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HIR6010858.1"
                     /translation="ILQFGYPGLAERFAQLKADFERFQATKDDKWCVGFVEGFEAYTT
                     QTALKAFAARVPDHPKTDRRFGELFKWHLLEEIEHRNVAYDIYEHLYGDQLFRARMCW
                     IAQTHIWRFILDCMKIMSPADVARFGETYRVSGLTRMAAVAAVAPMFLKTYMPWYSPH
                     KYVIPPEVAELSATLTAAAKSAA"
BASE COUNT          341 a          566 c          542 g          313 t
ORIGIN      
        1 catagcgtgt ccgcgctggt cgccgcgacg gcctctcgga ccgccgaccg ccaggtcgct
       61 gcgctgtcca gatcgaaata tcggacacgc cagccctgcc cgcgcagcgt ctgcgcgtag
      121 ctgcgcatgg cggaaagaac gaaggtgagc ttctgcttgt ggaaagcgta tcgccgacag
      181 aactgctcgt cctccaccat gacgaactcc gcatctgcat gctcacgcag atatcggggg
      241 tgaaagagct gattgccgaa gctgagaaag accttactca ccgacttgcc tccgttgctg
      301 atggatcacc agggcagctc acgcccgtcc cagttgaaga acctgcccga gtcctcgggc
      361 gatagatcgt gcacgacgga cagaatgtgc cgggcgcttt cgtcgggcgt acgcggatcg
      421 cgcccgcgaa cgaacggctc ggacagcggg gtcagggtgg ttccggggtg cacggccacc
      481 acgctgggac cgttgcgcag ccgaccgaac tcgatcgcca cgttgcgaac ccccatgttg
      541 agggctgctt tgctcatgcg gtagctgtac cagccgccga gagaattatc accaatgctc
      601 cccaccatgg cggagagcac ggcagcgacg gggcgctgat cgtggcgcag caggtctcga
      661 aaagcccgga tggcggccag cggggcgaac gcgttgacgc ggacggcgag ctggagattt
      721 tcaagctcca gctcagcgag cgacttttcg ggtcgatgag ggctcccggc cccgccatgc
      781 agaacgccgc tgcagcaaag gagcaggtgc acccgatcag tgagctgagc cacccgagac
      841 gcggcgctct ccagatccac cgggctggtc gtgtccgttt caaaccactc gataccatcg
      901 tccgacgatg gcctggtgcg ggacagcgcg aagacgcttg cgtagtcggg atgggcaacg
      961 agcgcccggc tgagcgcctc gcccagcccc cctgcggcgc cggccacgac ggcgactttt
     1021 ctcgacaaat cctttcccat tggctctttc tacaggaaaa aacctataca aacaacagaa
     1081 aaactggtgt ttggttccct gtatctcgaa tttgtctatg tattgtcgaa tttatcgaag
     1141 cggcccttcg cccggcggaa accgcaggcg cccggccggc cggcttctct ccccccctcc
     1201 ggtctggttc aggcagccga cttcgcggcc gccgtcagag tcgcggacag ctcggcaacc
     1261 tccggcggaa tcacgtactt gtgcgggctg taccagggca tgtaggtctt taagaacatg
     1321 ggcgcgaccg cagcaaccgc cgccatccgc gtaaggcctg aaacccggta ggtctcaccg
     1381 aagcgggcca cgtcagccgg cgacatgatc ttcatgcagt ccaggatgaa ccgccagatg
     1441 tgggtctgcg cgatccagca catcctggca cggaaaagct gatcgccgta gaggtgctcg
     1501 tagatgtcgt aggcgacgtt tctgtgctct atctcctcga gcaggtgcca cttgaagagc
     1561 tcgccaaagc gacggtctgt ttttggatga tccggaacgc gcgccgcgaa cgccttgagc
     1621 gcggtctggg tggtgtacgc ctcgaagcct tccacgaagc ccacgcacca cttgtcgtcc
     1681 ttggtggcct ggaaccgctc gaaatcggcc ttcagctgcg caaagcgctc cgccagcccc
     1741 gggtagccga actgcaaaat gg
//
LOCUS       DBNHQR010000291         3480 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1231437, whole genome shotgun sequence.
ACCESSION   DBNHQR010000291 DBNHQR010000000
VERSION     DBNHQR010000291.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1231437"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="AC16VR_06580"
     CDS             <1..586
                     /locus_tag="AC16VR_06580"
                     /inference="COORDINATES: protein motif:HMM:NF044986.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016421 - CoA carboxylase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HIR6010859.1"
                     /translation="AIAAAIEGRDARRKSARVMGTIPGGWRNSTMPMETVGYSAGAGE
                     VQLRYRARRDGMFRFVADESEKLVRVHGAGGAETDLEINGQRLRFLVGRIGDTWMVHG
                     SSGNVELVELPRYPLAGGGDLGSGMLAPMPGRVLETQVAEGTEVVKGQLLLVLEAMKM
                     EHRITAPRDGVVAKVHVNAGDQVENGQLLVTLAE"
     gene            602..1390
                     /locus_tag="AC16VR_06585"
     CDS             602..1390
                     /locus_tag="AC16VR_06585"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6010860.1"
                     /translation="MAGTQATLYETRKGAAWITLNRPANRNALSAPLVNELYDHLLVA
                     NDDAAVRSIVITGTGPAFCAGADLKEPPGQAIDGGRAVPYADVLSTILDSPKPVIAAV
                     NGAAFAGGLGLIGAADIVVTAEDVQFSFSEVRIGVIPAVISVVCLPKLGVHHGMKLIL
                     TGERFTGRRAVEIGFAHRAVPADELVDAVQEEIDMINLGGPNAMVECKKLVRRVPRLT
                     VEDGFREAAEWSSRMFRSDEAAEGMAAFREKRKPAWAVGEGEDE"
     gene            1383..3167
                     /locus_tag="AC16VR_06590"
     CDS             1383..3167
                     /locus_tag="AC16VR_06590"
                     /inference="COORDINATES: protein motif:HMM:NF018938.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HIR6010861.1"
                     /translation="MSDVIRIGNCSGFYGDRLEAAREMVEGGPIDVLTGDYLAELTMS
                     ILYSQIQSRGEDMGYVGTFLKQVRDVVAICIERGIKIVSNAGGLNPRSMAAEVDKILA
                     ELGLEATVAYIDGDDLLPDLERLQAEGEPFTNLDTGMALAETDEKALTANVYFGGWGI
                     KEALDRGADIVICPRVTDAAVVLGPAAWKFGWARNDYDALAGALAAGHIIECGAQCCG
                     GNYSFFEEVPSFRNVGYPIAEIEADGSFTITKHPGTGGLVSVGTVTAQLLYEISTPAY
                     FNPDVVAHFDTMTIEQTGEDRVRVSGCRGSSPPPTHKVCFNTLGPCKQSLEVLLTGLD
                     IEKKAEIYVDQVFHNLGGREQFDDVDVQLIRSDKEDPATNEEAHAALRITVTTRDPAK
                     LGRLLQAKVTELALAAIPGNTGRGTAGFNGSPVTVHWPALVDSRHVTERVHVGGATTD
                     VLPTQRLGLEEIYYQEVPVDVAPAPEGDRVTIPFGRLFGTRSGDKGGNANCGVWARTD
                     AAYGFLHRYLTVDQLKKLLPDMAGYEIERYDLPNLRAMNFYIRGVLGTGAASNHRIDK
                     QAKSLGEYLRAKLIEVPDALVAELARAE"
     gene            3220..>3480
                     /locus_tag="AC16VR_06595"
     CDS             3220..>3480
                     /locus_tag="AC16VR_06595"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6010862.1"
                     /translation="MSRLQDKVCLITGGARGQGAAEARAFAREGAKVWIADVLEDEGS
                     KLAAETGGEFRVLDVTSESGWQALVDEIIAVDGRIDVLVNNAG"
BASE COUNT          640 a         1075 c         1216 g          549 t
ORIGIN      
        1 ggccattgcc gccgccatcg aagggcgaga cgcccgccgg aagagcgcgc gggtcatggg
       61 aaccataccc ggcggctggc ggaactccac catgcccatg gagacggtgg gctactcggc
      121 aggagccggg gaagtgcagc tacgctaccg ggcgcgtcgt gacgggatgt tccggttcgt
      181 ggccgacgaa tcggagaagc tggttcgcgt tcacggagcc ggcggcgcag aaacggatct
      241 ggagataaac ggccagcgcc tgagattcct ggttgggcgg atcggagata cctggatggt
      301 tcacggtagc tcgggaaacg tggagctggt ggagctcccg cgctatccgc tagccggcgg
      361 cggtgatctg ggctcgggca tgctcgcgcc gatgcccggg cgcgtgctgg agacccaggt
      421 agccgaaggg acggaagtgg tcaaaggaca gctcctgctg gtcctggaag caatgaagat
      481 ggaacatcgc atcacggcgc cgcgggatgg ggtcgtggcg aaggttcacg ttaacgcggg
      541 cgatcaggtg gaaaacgggc agctgctcgt gaccctggcg gaataaggag ggctgaaccc
      601 agtggccggc acccaagcaa cactttacga gacgcgcaag ggggcggcct ggatcaccct
      661 gaaccgaccc gcgaaccgca acgcgctctc ggctcccctg gtgaacgagc tctacgatca
      721 ccttctggtc gccaatgacg acgccgccgt gcgttcgatt gtcattaccg ggaccggccc
      781 ggccttctgt gctggagcgg atctgaagga gccgcccggt caggccatcg atggcggtcg
      841 cgcggtgccg tatgcggacg ttctcagcac catcctcgac agcccgaagc ccgtcattgc
      901 cgccgtgaac ggcgcggcgt ttgccggggg tctcggtctc atcggggccg ccgatatcgt
      961 ggtgaccgcg gaagacgttc agttcagctt cagcgaggtc cgcatcggcg tcatcccggc
     1021 agtcatctcc gtggtctgcc tgcccaagct cggcgtgcac cacgggatga agctgatact
     1081 caccggcgag cggttcacgg ggcgccgcgc ggtggagatc ggcttcgccc atcgagcggt
     1141 cccggcagac gagctggtcg acgcggtgca ggaagagatc gacatgatca atctcggcgg
     1201 tccgaacgcg atggtcgagt gcaagaagct cgtgcgccgc gtgccccggc tgaccgtgga
     1261 agacggtttc cgcgaggccg ccgagtggag ctcccgcatg ttccgatccg acgaggcagc
     1321 cgagggcatg gcggcgtttc gcgagaagcg caagccggcc tgggccgtgg gagaaggcga
     1381 agatgagtga cgtgatacgt atcggcaact gcagcggctt ctacggcgac cggctggaag
     1441 ccgcccgtga aatggtggaa ggcggaccca tcgacgtgct caccggcgac tacctcgctg
     1501 agctgaccat gtcgatcctc tactcgcaga ttcagtcgcg cggcgaggac atgggctacg
     1561 tcggcacctt cctcaagcag gtgcgggacg tagtcgccat ctgcatcgag cggggaatca
     1621 agatcgtcag caatgcgggc ggcttgaatc cgcgcagcat ggcggccgag gttgacaaga
     1681 tcctcgccga gctggggctg gaggccaccg tcgcgtacat cgacggtgac gatctgctcc
     1741 ctgatctgga gcggcttcag gccgaaggcg agcccttcac caacctcgac acgggcatgg
     1801 cgctcgcgga aaccgacgag aaagccctca cggccaacgt gtatttcggc ggctggggta
     1861 tcaaggaagc tctcgatcgg ggcgccgaca tcgtcatctg cccgcgcgtg acggacgcgg
     1921 ccgtggtcct cggtcctgcc gcgtggaaat tcggctgggc ccgtaacgac tacgacgcgc
     1981 tggccggcgc gctggccgcc ggtcacatca tcgagtgcgg cgcccagtgc tgcggcggaa
     2041 actactcgtt cttcgaggag gttcccagct ttcgaaacgt cggctacccc attgcggaga
     2101 tcgaagccga cggcagcttc acgatcacga aacatcccgg gaccggcggc ctggtctcgg
     2161 tgggcacggt gacggctcag ctgctctacg agatctcaac gcccgcctac ttcaatccgg
     2221 atgtggtggc ccacttcgac acgatgacca tagagcagac gggtgaggat cgcgttcgtg
     2281 tcagcgggtg ccggggcagc agcccgcccc cgacccacaa ggtctgcttc aacacgctgg
     2341 gtccttgcaa gcagagcctg gaggtgctcc tcaccgggct cgacatcgag aagaaggccg
     2401 aaatctacgt cgaccaggta tttcacaacc ttggcgggcg cgagcagttc gacgacgtgg
     2461 acgttcagct gatccggagc gacaaggaag atcccgctac caacgaggaa gcccacgctg
     2521 cgctcaggat cacggtgacg acgagggacc cggcgaagct cgggcgcctg ctgcaggcta
     2581 aggtcacgga gcttgcgctt gccgccatcc ccggcaacac cgggcgcgga acggcgggct
     2641 tcaacggctc gccggtaacc gttcactggc cggccctcgt cgacagccgc cacgtgacgg
     2701 agcgcgtgca cgttggagga gcgaccaccg acgtcctgcc cacccagcgt ctgggcttgg
     2761 aggagatcta ctatcaggag gtccccgtcg acgtcgcgcc ggcgcccgag ggggacaggg
     2821 tcacgatacc gttcggccgc ctgttcggga cccgttcggg tgacaagggg ggtaacgcga
     2881 actgcggcgt ctgggctcgc accgatgccg cgtacggctt tctgcaccgt tacctgaccg
     2941 tcgaccagct gaagaagctg ctgccggaca tggccggcta cgagatcgag cgctacgacc
     3001 tgccgaatct gcgggccatg aacttctaca ttcgcggggt gctgggcacc ggagccgcgt
     3061 cgaatcaccg gatcgacaag caggctaaat ccctgggcga gtaccttcgt gccaagctca
     3121 tcgaggtgcc cgatgcgctg gtggcggagc tggcccgggc ggaatgagtc ccgcaccgat
     3181 gcacggcggg gccagatggg aaaacgaagg agaagccaag tgagccgact tcaggacaag
     3241 gtctgtctca tcacgggcgg cgcccgcggt cagggcgcgg cggaagcgcg cgcgttcgcc
     3301 cgggaaggcg cgaaggtctg gatcgccgac gttctggagg acgaaggctc gaagctggcg
     3361 gccgagaccg gcggcgagtt ccgggtgctc gacgtcacct cggagagcgg ttggcaggcc
     3421 ttggttgacg agatcatcgc cgtcgacggg cgaatcgacg tgctcgtcaa caatgccggg
//
LOCUS       DBNHQR010000292         2651 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1295214, whole genome shotgun sequence.
ACCESSION   DBNHQR010000292 DBNHQR010000000
VERSION     DBNHQR010000292.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1295214"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="AC16VR_06600"
     CDS             <1..573
                     /locus_tag="AC16VR_06600"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF015647.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HIR6010863.1"
                     /translation="SDPRESITGFDTGPGNCLMDGWIDHCRSMPYDREGDWAREGSPS
                     PALLAAMLADPYGLRTPPKSTGREYYNLEWLSRHPGTGHLSEADVQATLLEFTARSIA
                     DAVDRWGGRAERWLVCGGGRHNRLLMERLAQLTPSPIETTDAHGVDGDALEAGAFAWL
                     AHQTMASLPGNAPGVTGASGERILGGIYRP"
     gene            complement(592..936)
                     /gene="erpA"
                     /locus_tag="AC16VR_06605"
     CDS             complement(592..936)
                     /gene="erpA"
                     /locus_tag="AC16VR_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009408529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HIR6010864.1"
                     /translation="MTGQLDIDISFTERAAAKVRALIADEENADLKLRVFITGGGCSG
                     FSYGFTFDEEVAEDDAVVEREGVSMLVDSLSYQYLLGAEVDYKEGLQGAQFVVQNPNA
                     SATCGCGNSFAI"
     gene            complement(1051..1500)
                     /locus_tag="AC16VR_06610"
     CDS             complement(1051..1500)
                     /locus_tag="AC16VR_06610"
                     /inference="COORDINATES: protein motif:HMM:NF016408.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactofilin family protein"
                     /protein_id="HIR6010865.1"
                     /translation="MARTKVRDGVTLIAANTEVTGNVKFNGQLYVSGRVNGNILAAEG
                     CEATVVVSEEGVVSGEIRVPNVVINGTVEGDISASERIELACKARVKGSVYYALVEMQ
                     LGAQVDGQLVHEHPQTAASEDAHKAGAKRRNPLGIIKQLPKAGATAS"
     gene            complement(1562..2320)
                     /locus_tag="AC16VR_06615"
     CDS             complement(1562..2320)
                     /locus_tag="AC16VR_06615"
                     /inference="COORDINATES: protein motif:HMM:NF042896.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6776 family protein"
                     /protein_id="HIR6010866.1"
                     /translation="MARDPLKGIIKGEGQVRVVAHSPRRRLLTSLTVALVCAAGAAGG
                     FYLGESKAGLDAGYIVALEKMDRANAVEIAELRSRLIDANLARSVESQAARELRDDIR
                     ILRDEVAGLQEEVTFYKSIMAPDSIERGLQIAEFQLVPGEAVNEFTYHLLLTQVEARR
                     DWIQGTVTLDVVGRRTPDPSRKDVELVLSLTEIGQTDTYPLSFRFRYFQDLTGSMALP
                     DGFLPEAVVVTARKRGARSEQLQRKFAWAMGEAE"
     gene            2361..>2651
                     /locus_tag="AC16VR_06620"
     CDS             2361..>2651
                     /locus_tag="AC16VR_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010867.1"
                     /translation="MIRFTSSSSAPSAEIGALRLPNKVSPEFFRNRLADIEALPQLAL
                     LAVLAGLITGCVILLFRAAVELILGGWLLPEGSESFEALSILERLALPIAGAG"
BASE COUNT          456 a          878 c          800 g          517 t
ORIGIN      
        1 tcggaccctc gcgaaagcat cacgggtttc gataccggac cgggcaactg cctgatggat
       61 ggctggatcg accattgccg atcgatgccc tacgaccgcg agggcgattg ggcaagggaa
      121 ggctccccca gcccggccct gctcgcagcc atgctcgcgg acccctacgg cttgcggacg
      181 ccgccgaaaa gcacgggtcg cgagtactac aacctcgaat ggctcagccg acatcccggc
      241 accggacacc tcagcgaagc cgacgttcag gccacccttc tcgagtttac ggcccgcagc
      301 atcgccgacg cggtggatcg ctgggggggg cgggcggaga gatggctcgt ctgcggcggt
      361 ggacgccaca accggctgct catggaaagg ctggcccagc tgacgccctc cccgatcgag
      421 acgaccgatg cccacggcgt ggacggcgat gctctggaag ccggtgcttt cgcctggctg
      481 gctcaccaga ccatggcaag ccttccgggc aacgcgcccg gcgtcacggg tgcctcggga
      541 gagcgcattc tcggcggtat ctaccggccg tgacggctac ccggatcctt ctcatatggc
      601 gaaggagttg ccgcatccgc aggtagcgga cgcgttggga ttctgcacca cgaactgcgc
      661 gccctgcaga ccttctttgt agtcgacctc cgcgcccagc aggtactgat agctgaggct
      721 gtcgacgagc atgctaacgc cttcgcgctc gacgacggca tcgtcttcgg ctacttcctc
      781 gtcgaatgtg aagccgtagg aaaagccgct gcatccacct ccggtaatga agacccggag
      841 tttgagatcc gcattctcct catccgcgat gagtgcccgc actttcgcgg ccgcgcgctc
      901 ggtgaagctg atgtcgatgt cgagctgtcc agtcattgag ttacccggtg acgctctcac
      961 tggttccccg gccgcaagat gaacgaggcc cgaggggcgg cgagggcgga aaattatcga
     1021 agtaccccca gaccgggtca agggtgccct tcaggacgcg gtcgcgcccg ccttaggcag
     1081 ttgcttgatg atccccaggg gattcctgcg ctttgctccg gccttatgag catcctcaga
     1141 cgcggccgtc tggggatgtt cgtgaaccag ctggccgtcg acctgggccc cgagctgcat
     1201 ctctaccagc gcataataca cgcttccttt gactctggct ttgcaggcaa gttctatccg
     1261 ctcggaagca gagatgtcgc cttcgacggt gccgttgatg accacgttgg gaacgcggat
     1321 ctcgccggaa accacaccct cctcgctcac caccacggtg gcttcgcagc cttcggcggc
     1381 gagaatgttg ccgttcaccc gaccgctgac gtagagctgc ccgttgaact tcacgttccc
     1441 ggtgacttcg gtattcgctg cgatcagggt tacgccgtct cggaccttgg ttctcgccat
     1501 gatgaccctt cgttcctggg gggccctggc gcgctctcgc cgtccgttta ggaaacggcg
     1561 ctcattcggc ttcgcccatg gcccaggcaa actttcgctg caactgctcg gagcgcgcac
     1621 cgcgtttccg ggcggttacc accaccgctt ccggcagaaa tccatcgggt aacgccatgc
     1681 tcccggtgag gtcctggaag taccggaacc ggaaactcag aggatatgtg tcggtttgcc
     1741 cgatttctgt gagggagagc accagttcta catctttccg tgacggatcc ggcgttcttc
     1801 ggcccaccac atcgagggtc acggtgccct gaatccaatc ccggcgggcc tccacctggg
     1861 tcagcagcag gtgataggta aattcgttga ctgcttcgcc gggaacgagc tgaaattccg
     1921 caatctgcag gccccgctcg atgctgtccg gagccatgat gctcttatag aaagtcacct
     1981 cttcctggag accggccacc tcgtccctga ggatccggat gtcgtcgcgc agctcgcgcg
     2041 ccgcctgtga ctccacgctt cgggcgagat tggcgtcgat gagcctgctg cggagctcgg
     2101 cgatctcaac cgcgttcgca cggtccatct tctccagggc aacgatgtag cctgcatcca
     2161 gccccgcctt ggattcgcca aggtagaacc cacccgcggc accggccgcg cagaccaggg
     2221 cgacggtcaa tgacgtgagc aggcgcctgc gcggcgagtg cgccaccacg cgcacctgtc
     2281 cctcgccttt gatgatgcct ttcaatgggt cacgtgccat acgggatgat ctgcctcggg
     2341 ccattccaga gactatacta gtgatccgat tcaccagttc gagctctgca ccgtcggcag
     2401 agattggggc cctgagatta ccaaataaag tctccccgga gttcttccgc aatcggctgg
     2461 cggacatcga ggcgcttcca cagctggcgc tactggccgt tctggccgga ctgatcaccg
     2521 gctgcgtcat cctgctcttc cgggcggccg tcgagctcat cctcgggggc tggctgcttc
     2581 ccgaaggcag tgaatccttc gaagcgctga gcatcctgga gcgcctggcc ctgcccatcg
     2641 ccggcgccgg c
//
LOCUS       DBNHQR010000293         1927 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1547476, whole genome shotgun sequence.
ACCESSION   DBNHQR010000293 DBNHQR010000000
VERSION     DBNHQR010000293.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1547476"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1339)
                     /locus_tag="AC16VR_06625"
     CDS             complement(<1..1339)
                     /locus_tag="AC16VR_06625"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6010868.1"
                     /translation="MVEATANPAADESALDTFPKLLLDRAVRYRDAHAAREKEFGIWQ
                     SWTWHELAEEIQDLAAGLAELGFQRGDKIAIIGDNRPRLYGAMCAAQALGGVPVPMYQ
                     DSVANELQFVVDHADVRFAIGENQEQVDKLPEIKESCPKLEVIIFDDARGLRKYEQDF
                     LHSYAGVQKLGRGRRERDSAFLNDEIAKGAGSDIAIILYTSGTTGNPKGVMLSHDNVL
                     VTARNSVERDGLRQSDEVLAYLPMAWVGDNIFSFGQSYVAGFCVNCPESSETVMHDLR
                     EVGPTYFFAPPAIYENLLTNVMIRMEDAAKLKRRLFHYFMGISKRVGVMILDGKPVSM
                     KDRLLYRLGEILIYGPLKNTLGFSRIRIAYTAGEAIGPDIFDFYRSLGINIKQLYGQT
                     EAAVFVTMQPNGEIKPDTVGTPAPQVEIKITDSGEVMYRSPGVFQCYYKNPEAT"
     gene            complement(1565..>1927)
                     /locus_tag="AC16VR_06630"
     CDS             complement(1565..>1927)
                     /locus_tag="AC16VR_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003250091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6010869.1"
                     /translation="ESGERGVIVNTASVSAFEGQIGQAAYSASKGGVAAMTLPIAREL
                     ARFGVRVVTIAPGLMSTPMLHGMPEEVQQSLGAQVPFPGRLGRPEEYAALVVHIAENS
                     MLNGEVIRLDGAIRMQPK"
BASE COUNT          334 a          639 c          595 g          359 t
ORIGIN      
        1 ccgtcgcctc gggattcttg tagtaacact gaaagacacc ggggctgcgg tacatgacct
       61 cgccgctgtc ggtgatcttg atctccacct ggggtgcggg tgtgccgacg gtgtccggct
      121 tgatctcgcc gtttggctgc atggtcacga agacggccgc ctcggtctgt ccgtagagct
      181 gcttgatgtt gatacccagc gatcggtaga aatcgaagat gtcgggtccg atggcttcgc
      241 ccgcggtgta ggcgatgcgg atgcgggaaa agccgagcgt gttcttcagg ggaccgtaga
      301 tcaggatctc gccgagccga tacagcagcc ggtccttcat ggacaccggc ttcccgtcca
      361 ggatcatgac gccgacgcgc ttggagatgc ccatgaagta gtggaacaac ctgcgcttca
      421 gcttggccgc atcctccatc cggatcatga cgttggtgag caggttctcg tagatcgccg
      481 gcggcgcgaa gaagtaggtc gggcccacct cgcggagatc gtgcatcacg gtctcgctgc
      541 tctccggaca gttgacgcag aatcccgcca cgtaggactg gccgaaggag aagatgttat
      601 cgccgaccca tgccatcggc agataggcga gaacctcgtc gctctggcgt agcccgtcgc
      661 gctccaccga gtttctcgcc gtcacgagga cgttgtcgtg gctcagcatg acccccttgg
      721 gattcccggt cgtcccggac gtatagagga tgatcgcgat gtccgagccc gcgcccttgg
      781 cgatctcgtc gttcaggaac gcggagtccc gctcgcggcg cccgcgtccc agcttctgta
      841 cgcccgcgta ggagtgaagg aagtcttgct cgtacttcct caagccccgg gcatcgtcga
      901 agatgatcac ctcgagcttc gggcagctct ccttgatctc cggcagcttg tccacctgct
      961 cctggttctc gccgatggcg aaacgcacgt cagcgtgatc gacgacgaac tgcagctcgt
     1021 tcgcgacgga gtcctggtac atcgggacgg gcacgccgcc cagcgcctgg gccgcgcaca
     1081 tggcgccgta gagccgcggg cggttgtcgc cgatgatggc gatcttgtcg ccgcgctgga
     1141 atcccagctc cgcgagcccg gcggcgagat cctgaatctc ctcggccagc tcgtgccagg
     1201 tccacgactg ccagatgccg aactctttct cccgggccgc gtgggcgtct cgatagcgca
     1261 cggcgcgatc gaggagcaac ttcggaaatg tatccagcgc ggactcgtcc gccgccgggt
     1321 tggccgttgc ctcaaccatc aactcactcc cctcaccccg gaatcacgat agacgcgcgc
     1381 cgccgatgac cccctggaat cgaccaggtg ggaaaccctg agaccgggac gaatttacct
     1441 agaagagaac tcctgacgac tttctaatta tttgaattca cgggtcattt cctgccaccg
     1501 gcgctccggc acgggcccgg cggcggcagg tgctcatcct aaccgcatag cggcccccat
     1561 gccatcactt cggctgcatg cggatagcgc catccaggcg gatgacctcg ccattcagca
     1621 tggaattctc cgcgatgtgc acgaccagcg ccgcgtactc ctccggtcgg ccgaggcggc
     1681 cggggaaagg cacctgggca cccagactct gctgcacctc ctcgggcatg ccgtggagca
     1741 tcggcgtgct catcagaccc ggggcaatgg tcacgacccg cacaccgaaa cgggccagct
     1801 cccgggcgat tggcagggtc atggcggcga caccgccctt cgacgcgctg taggcggcct
     1861 gtccgatctg gccctcgaag gcggacaccg aggcggtatt cacgatcacg ccccgctcgc
     1921 cgctctc
//
LOCUS       DBNHQR010000294         2631 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_788961, whole genome shotgun sequence.
ACCESSION   DBNHQR010000294 DBNHQR010000000
VERSION     DBNHQR010000294.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_788961"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="AC16VR_06635"
     CDS             <1..753
                     /locus_tag="AC16VR_06635"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014597394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HIR6010870.1"
                     /translation="AYRAALVGFLTPRAAGLDADSQRRLGSNPLRILDSKVEQTQDLL
                     AGAPSLEDFIDDEGKAHFSGLCELLEQLDVPFEINPKLVRGLDYYTHTVFEWKTDALG
                     AQDTICAGGRYDGLVELLGGRPTPGAGFALGVERVLLLHEQRFQGAGFAAAADVYCCV
                     LDDRRQSEVFAIAQKLRDALPGLRIRTHAGGGKLKSQMRRADASGARLAVLVGEDEIA
                     KGVVSLKYLRESREQESVPAADLETVLAEYFA"
     gene            785..1486
                     /locus_tag="AC16VR_06640"
     CDS             785..1486
                     /locus_tag="AC16VR_06640"
                     /inference="COORDINATES: protein motif:HMM:NF021492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0044877 - protein-containing complex
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HIR6010871.1"
                     /translation="MSDYLTDEEQVAKLKSWWDENGVSVIVTLVLVVAGVVGWRWYES
                     YHETRVARVSDLYEEFLVSEGDARDASAASIAEEAPDSAYQTLVMLRRSQESMESGDY
                     EAAEAFLVEAVEAAPEPVLADVARVRLARVQQQLDRLDDALETLSGVLGEGYRSQVAE
                     MKGDIHLARGERALAYEAYASALAAVGDNQSRPVLEMKAADTADAVTGAQPAAAPEAG
                     QGLESPGETAETPDA"
     gene            1479..>2631
                     /gene="bamB"
                     /locus_tag="AC16VR_06645"
                     /gene_synonym="yfgL"
     CDS             1479..>2631
                     /gene="bamB"
                     /locus_tag="AC16VR_06645"
                     /gene_synonym="yfgL"
                     /inference="COORDINATES: protein motif:HMM:TIGR03300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HIR6010872.1"
                     /translation="MRKILLLLLTFSLTGCSWFSWLPWVGDKDDEKKKLEPAKLVDFD
                     AEVNVDRVWRASIGEGLGKKYLRLDPLVLADRVYAADGYGIVEALDRFSGKRVWRSRI
                     GRVEEGLLGSINFFDREDPSFVSGGVGGGAGYVFMGTTGGDVVALSAADGSEAWRSHV
                     GSEVLAPPVGERDLVFVQTQDGRLLALESETGDIRWSFDNQVPVLTLRGTGTPVVAGN
                     VVFAGFSNGMLAALRAESGEPVWEQRIMLPEGRSELERMVDVDGTPLLLGPILYVVAY
                     QGKLKALRRTDGAMLWEMDVSSFLDLAEGYGQIYVVDDGDVVTAVDQQSAEIVWQQPG
                     LARRKLTSPVAFSNYVAVGDDQGYLHILAQSDGRFVGRRKLDGDGLRSSM"
BASE COUNT          445 a          765 c          946 g          475 t
ORIGIN      
        1 gcttatcggg cagctctggt gggctttcta acgccgcggg cggccggtct ggatgctgac
       61 agccagcgcc ggctcggctc gaacccgctt cgcattctgg acagcaaggt cgagcagacc
      121 caggacctgc tggccggggc gccctccctg gaggacttca tcgacgacga aggaaaggcg
      181 cacttctccg gtctgtgcga gctgctcgag cagctcgatg ttccgtttga aatcaatccg
      241 aagctggtcc gtggcctgga ttactacacc cacaccgtgt tcgagtggaa aaccgacgcg
      301 ctcggagccc aggacacgat ctgtgcgggt ggccgttacg atgggctggt cgagctgctc
      361 ggcgggcggc cgacgccggg cgcgggcttt gccctggggg tggagcgcgt tctgctcctt
      421 cacgagcagc ggttccaggg ggccggcttc gcggctgcgg ccgacgttta ctgctgcgtg
      481 ctggacgacc ggcgccaatc ggaggtattc gccatcgcgc agaaattgcg tgatgcgctc
      541 ccaggtctca gaatccgcac ccacgccggt ggcggcaagc tgaagagcca gatgcgacgc
      601 gccgatgcca gcggcgcgcg cctcgcggtt ctcgtcggcg aggacgagat cgccaagggc
      661 gtggtgtcgc tgaaatacct tcgcgagagc cgggagcagg aaagcgtccc ggccgctgat
      721 ctggaaacgg tgctggccga gtacttcgcc tgaacctggc gagcgaatcg aaaaggagat
      781 tggggtgtcg gattacctga ccgacgaaga gcaggtcgca aagctcaaat catggtggga
      841 cgagaacggc gtgtccgtca tcgtgacgct cgttctggtc gtcgccgggg tggtcggttg
      901 gcgatggtac gaaagctacc atgagacgcg ggtcgcgcga gtttcggatc tctacgagga
      961 gtttctggtc agcgaagggg acgccaggga cgcaagcgcc gcgtccatcg ccgaagaggc
     1021 acccgacagt gcctatcaga cgctcgtcat gctgcgccgc tcgcaagagt ccatggagag
     1081 cggcgactac gaagccgccg aggcgttcct cgtcgaggcg gtggaagctg ctccggaacc
     1141 cgttctggcg gacgttgccc gggtgcggct cgcgcgggtg cagcagcagc tggaccgtct
     1201 cgatgatgcg ctcgaaacgc tttccggggt gcttggcgaa ggatatcggt cacaggttgc
     1261 cgagatgaag ggggatatcc atctggcccg cggggagcgc gcccttgctt acgaagcata
     1321 cgcttcggcg cttgcggcgg tgggtgacaa ccagtcccgt ccggtgctgg agatgaaagc
     1381 cgccgatacc gccgatgccg tgaccggagc gcagcccgct gcagcgccgg aagccggaca
     1441 gggcctcgaa tcccccgggg agactgcaga aacgcccgat gcgtaaaatt ctactgctcc
     1501 tgctgacttt ctcccttacc ggctgcagct ggttttcctg gttgccgtgg gtgggcgaca
     1561 aggacgacga gaagaaaaag ctcgagccgg ccaagctggt ggacttcgat gccgaggtga
     1621 acgtcgaccg ggtctggcgg gcctccatcg gtgagggtct cggcaagaag tatctgcgac
     1681 ttgatcccct ggtcctcgcc gaccgggtgt acgccgcgga cggctatggc atcgtggaag
     1741 cgctcgaccg tttttccggc aagcgggtct ggcggtcgcg cataggtcgc gtcgaagaag
     1801 gtctcctggg ctcaatcaac ttcttcgatc gtgaagatcc gagtttcgtg agtggcgggg
     1861 tcggcggtgg tgcaggctac gttttcatgg gcacgacggg cggtgatgtg gtggcgctgt
     1921 ccgctgcgga cggttcggaa gcctggcggt cccacgtggg cagcgaggtg ctcgcgccac
     1981 cggtgggcga gcgcgatctg gtgttcgtgc agactcagga cggcaggctg ctggcgctgg
     2041 aatccgagac cggggacatt cgctggagct tcgacaacca ggttccggtg ctgaccttga
     2101 gaggcaccgg caccccggtg gtggccggta acgtggtgtt tgcgggcttt tccaacggga
     2161 tgctcgccgc tctgcgcgcc gaaagcggcg agcccgtctg ggagcagcgg atcatgcttc
     2221 cggaaggaag gtccgagctg gaacgcatgg tggacgtgga cggcacgccc ctgctcctgg
     2281 gtccgatcct ctacgtcgtg gcttaccagg gtaagctcaa ggcgctgcga cgaaccgacg
     2341 gagccatgct gtgggagatg gacgtgtcca gcttcctgga tctcgccgaa ggatacggcc
     2401 agatctacgt ggtggacgac ggggacgtgg tgacggccgt cgaccagcag tcggcggaga
     2461 tcgtctggca gcagccgggc cttgcgcgtc gtaagctcac gagccccgtt gctttcagca
     2521 actatgtcgc ggtgggcgac gatcagggat atctgcacat ccttgcgcag agtgacgggc
     2581 gcttcgtcgg gcgccgcaag ctcgacgggg acgggctgcg gtcgtcgatg g
//
LOCUS       DBNHQR010000295         1351 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_789022, whole genome shotgun sequence.
ACCESSION   DBNHQR010000295 DBNHQR010000000
VERSION     DBNHQR010000295.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_789022"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="AC16VR_06650"
     CDS             complement(<1..976)
                     /locus_tag="AC16VR_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2333 family protein"
                     /protein_id="HIR6010873.1"
                     /translation="MKINWRFWASSNDADVTDGRLATAGRIGLGAVAAYLLVCVLLGW
                     WWDKEPDVFAVRENASSRAELGNEPLVTGYITTATLIRLGETLLDKRGGYLSNDVFPP
                     GVWMDNIPNWEFGVLTHIRDATRTYRNELSRSQSQSAEDPDIVRAEGKFFIDNASWFI
                     PQAESEYRDGIKALSSYLQRLADPTQPDAQFYARADNLQQWLQIVEARLGSLSQRLSA
                     SVGKRQLDVSLAGDAAASQATESPREQEVKTPWLEIDDVFYEARGQTWALLHLLRAME
                     QDFGDVLDKKNARVSLQQIIRELEPTQDTVWSPIILNGDGLGLLANHSL"
     gene            complement(973..>1351)
                     /locus_tag="AC16VR_06655"
     CDS             complement(973..>1351)
                     /locus_tag="AC16VR_06655"
                     /inference="COORDINATES: protein motif:HMM:NF016221.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HIR6010874.1"
                     /translation="PALAGSELVVTDARVRALLPGQRHTVAYFAIENRGPASVTLVSA
                     ESAAAGAMEIHESRRDGDMYRMRRRAEVEIPARTTVRFDPGGLHLMLFDVQALGEQME
                     IELRTSDGRRLKAIFNRIAAGAE"
BASE COUNT          212 a          434 c          457 g          248 t
ORIGIN      
        1 ccagcgagtg gttggccagc aacccgagcc cgtcgccgtt gaggatgatg gggctccaga
       61 ccgtgtcctg ggtgggctcg agctcccgga tgatctgctg caggctgact cgggcgttct
      121 tcttgtccag cacgtcgcca aagtcctgct ccatggcccg gagcaggtgg agcagcgccc
      181 aggtctggcc tcgggcctcg tagaagacgt cgtcgatttc cagccacggc gtcttgacct
      241 cctgctcccg cggggactca gtggcctggc tggccgcagc atctccggcc agggagacgt
      301 cgagctgccg tttgcccacg ctggcggaca agcgctggga caggctgccg agccgggctt
      361 cgacgatctg cagccactgc tggaggttgt ctgcccgcgc gtagaactgg gcatcgggct
      421 gggtggggtc ggccaggcgc tgcaggtaag aggagagcgc tttgatgccg tcgcgatatt
      481 ccgactccgc ttgagggatg aaccagctcg cgttgtcgat gaagaacttg ccctccgctc
      541 tgacgatgtc ggggtcttct gccgactggc tctgggagcg cgaaagctcg ttgcggtagg
      601 tgcgggtggc gtcccgtatg tgggtgagca ccccgaattc ccagttcgga atgttgtcca
      661 tccacacgcc cggcggaaag acgtcgttgg aaagatagcc accgcgtttg tcgagcaggg
      721 tttcgcccag gcggatcagg gtggcggtgg tgatgtaacc ggtgaccagc ggctcgttgc
      781 cgagctccgc gcggctcgat gcgttctcgc gaacggcaaa cacgtcgggt tccttgtccc
      841 accaccagcc cagcagcacg cagacgagca ggtaggcggc aacggcgccc agcccgatgc
      901 gaccggcagt tgcgagcctc ccgtcggtga cgtccgcatc gttcgaagaa gcccagaatc
      961 gccagttgat cttcattccg ctcccgctgc tatgcgatta aagattgcct tcaggcggcg
     1021 cccgtcgctc gtgcgcagct cgatttccat ctgctcgccg agcgcttgga cgtcgaacag
     1081 catcagatgc agcccgcccg ggtcgaagcg tacggtggtc cgggctggaa tctcaacctc
     1141 tgctcggcgc cgcatccggt acatgtcgcc gtcgcggcga gactcgtgaa tctccatggc
     1201 cccggcggcc gcgctctcgg cgctcaccag ggtaacgctt gcggggccgc ggttttcgat
     1261 cgcgaagtag gcaaccgtat ggcgctggcc gggcagcagc gcccggaccc gcgcatcggt
     1321 cacgaccagc tcggaacccg ccagggcggg c
//
LOCUS       DBNHQR010000296         2065 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1611664, whole genome shotgun sequence.
ACCESSION   DBNHQR010000296 DBNHQR010000000
VERSION     DBNHQR010000296.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1611664"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2021)
                     /locus_tag="AC16VR_06660"
     CDS             complement(<1..2021)
                     /locus_tag="AC16VR_06660"
                     /inference="COORDINATES: protein motif:HMM:NF023649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HIR6010875.1"
                     /translation="MTIDHVRGRLLVLPLLLVLLALWGCSRTEEPAPAEPPEPSGAVS
                     AAEIERNSARNAYFGDLHVHTRLSFDAFIFGTRSTPDSAYAYALGEPIDHPAGFQMQL
                     DRPLDFLGVTDHAMYMGMLEEMSNPETRAGQHPIAVGIREAETPASRLEAFQAMFPYL
                     RKQLDGPDDLLDPAVLRSAWQETVAAAERHNDPGRFTSFVAYEYTAGGAERQNLHRNV
                     VFRGAAPELPFSSMDSGNPEDLWAWMDAQRGQGVEALAIPHNSNGSDGLMFELTRVDG
                     SPMDAAYAEQRMRNEPLVEITQVKGTSDAHPLLSPNDEWADFELMEVKVATVEPSRAA
                     GSYVREAYLNGLAMQAEQGFNPYRFGLIGSSDTHVGAGSFDEANYWSKVGIVDATSVQ
                     RGSVPMDEPDADGNLYVGGDVATFQDWGASGLAGVWAEANTREDIYDAFRRKETFATT
                     GPRMQVRFFAGFGLEAAEQAGPDRISALYRDGVPMGGDLVGGDADTAPHFFLWALSDP
                     ASARLQRLQVIKGWIDAEGRSQEKVFDVACSDGLEVDEATHRCPDNGARVNLADCSIS
                     QNVGAAELESLWKDPEFDAGQMAFYYVRALENPTCRWSTWDAVRAGVEPRADLQATIQ
                     ERVWSSPIWYVPGGLAGDQPEAEPAAASLVGTRWLWVGSTIGSADVEVA"
BASE COUNT          358 a          710 c          649 g          348 t
ORIGIN      
        1 tccgccacct cgacatccgc cgagccgatc gtcgacccca cccagagcca acgggtccca
       61 acgaggcttg ccgccgccgg ctcggcttcc ggttgatcgc ctgccaagcc gccggggacg
      121 taccagatcg gcgatgacca gacgcgctcc tgaatcgtag cctgcaagtc cgctctgggt
      181 tccaccccgg cccggaccgc gtcccaggta gaccagcgac aggtggggtt ctccagcgcg
      241 cgcacgtagt agaaggccat ctggccggca tcgaactcgg gatccttcca gagactctcc
      301 agctcggcgg cgcccacgtt ctggctgatg gagcagtcgg cgagattcac gcgggcgccg
      361 ttgtccgggc atctgtgggt cgcctcgtcg acctcgaggc cgtccgagca cgccacgtcg
      421 aacacctttt cctggcttct tccctctgcg tcgatccatc ccttgatgac ctgaagacgc
      481 tgcaagcgcg ccgacgccgg gtcactcaga gcccagagga aaaagtgcgg cgcggtatcg
      541 gcgtcgccgc cgactagatc gccgcccatg ggcacgccgt cgcgataaag cgcgctgatc
      601 cggtcgggcc ctgcctgctc cgctgcctcc agcccgaagc ccgcgaagaa ccggacctgc
      661 atgcgcgggc cggtggtggc gaaggtctcc ttacgacgga acgcgtcgta tatgtcttca
      721 cgcgtgttcg cctccgccca taccccggcg agcccggacg cgccccaatc ctgaaacgtt
      781 gcaacatctc cgcccacgta gaggttgccg tcggcgtcgg gctcgtccat gggcacggag
      841 ccgcgctgga cggaggtcgc atccacgatg ccgaccttgc tccaatagtt ggcctcgtcg
      901 aaggagcccg cgccaacgtg ggtgtccgac gagccgatga ggccgaaccg gtacgggttg
      961 aatccctgct ctgcctgcat ggccaggccg ttgaggtaag cctctcgaac atagctgccg
     1021 gccgccctgc tgggctccac ggtcgccacc ttgacctcca tcagctcgaa gtcggcccac
     1081 tcgtcgttgg gcgaaagcag gggatgggcg tcagacgtgc ccttgacctg cgtgatctcc
     1141 accaggggct cgttgcgcat ccgctgctcg gcataagccg catccatggg cgagccgtcc
     1201 acacgggtca gctcgaacat cagcccgtcg gagccgttgg agttgtgagg aatcgccagc
     1261 gcttccacgc cctggccgcg ctgggcgtcc atccacgccc acaggtcctc cgggttgccg
     1321 gagtccatgc tggagaacgg cagctcgggg gcggcgcccc gaaaaaccac gttgcggtga
     1381 aggttctgcc gctcggctcc ccctgcggtg tattcgtacg ccacgaagct ggtaaaccgg
     1441 cccggatcgt tgtggcgctc ggcagcagcc acggtttcct gccacgcaga gcgaagcacc
     1501 gcgggatcga gcagatcgtc cggaccatcg agctgcttgc gcagataagg aaacatggcc
     1561 tggaacgcct ctagacgcga tgcaggagtc tccgcctcgc ggatgccaac cgctatcggg
     1621 tgctggccgg cccgggtttc ggggttgctc atctcctcca acatgcccat gtacatggcg
     1681 tgatccgtca ctcccagaaa atccaggggc cggtccagct gcatctggaa accggcggga
     1741 tgatcgatgg gctcgcccag agcataggcg taagccgagt ccggcgtgga gcgcgtgccg
     1801 aagatgaaag cgtcgaaaga cagcctcgtg tgcacgtgca ggtcgccgaa gtaggcgttg
     1861 cgggcggagt ttcgctcgat ctcggccgcc gacacagcac cgctcggctc gggcggctcg
     1921 gcaggagcgg gttcttcggt tcgagagcac ccccacagcg ccagcaggac gagcagtagg
     1981 ggcaaaacca gcagccttcc cctcacatga tcgatcgtca tcctcacccc tccgaagatc
     2041 tccacgtttc agataatgta gcagg
//
LOCUS       DBNHQR010000297         2402 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1041731, whole genome shotgun sequence.
ACCESSION   DBNHQR010000297 DBNHQR010000000
VERSION     DBNHQR010000297.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1041731"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="AC16VR_06665"
     CDS             complement(<1..657)
                     /locus_tag="AC16VR_06665"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6010876.1"
                     /translation="MDFHFASAWEAIADTVPDRTALICEGVTRTWADYDGRATRVAGL
                     LQAHGLGADTKVGLYLHNCNEYLEAQYGVFKLSGCPINVNYRYKAEELVYLLDNADAE
                     AVFYQGCYAPRIEEIRDQLPRVKLYVQVDDGTEDLLAGSVDYESSLDAAEPMARQKRD
                     PASVYMLYTGGTTGMPKGVMYPGGEFCYLLTAMGAGGRGLVPPQNLEALREYVAGIED
                     P"
     gene            931..1146
                     /locus_tag="AC16VR_06670"
     CDS             931..1146
                     /locus_tag="AC16VR_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010877.1"
                     /translation="MIIKDNAPAGPIARLSRITYPRASAANLMLLSVVIAAIGAIAYI
                     LHPDAAQPAPNTFHGAALLVSNWLLCG"
     gene            1174..1386
                     /locus_tag="AC16VR_06675"
     CDS             1174..1386
                     /locus_tag="AC16VR_06675"
                     /inference="COORDINATES: protein motif:HMM:NF015957.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HIR6010878.1"
                     /translation="MANSNLNNVRRRLRLDPRNGWIAGACAGFANRLQIDPAIVRVVV
                     VIAALFFPKTVIACYLLAWLVLDEAR"
     gene            complement(1411..2304)
                     /locus_tag="AC16VR_06680"
     CDS             complement(1411..2304)
                     /locus_tag="AC16VR_06680"
                     /inference="COORDINATES: protein motif:HMM:NF039714.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6279 family lipoprotein"
                     /protein_id="HIR6010879.1"
                     /translation="MIERMVVRLFALLAVLVLGSGCSVQFAYNNLDRLVRWGVNDYVN
                     MNDAQRNLFNEEFARLHYWHRTEHMPLYADFFEAVPTTLSDGVDAQELLALENTMMGW
                     AKEIVETGAPLAVAIMQTMTDEQVAGLPARLEESNREIEKTELGKSVEASQARWREEV
                     EDIFTRFAGRLTPAQQEYLVAQSVRYIPERVLWAEYRRRWQRDLMKLLQARSEPAFAD
                     RFAELAANREAYFGAELTAIFDNNENLGRETAAWLLNHMAERQSVRMYTRFVELAIDF
                     RELADDAPNWLPPEEPCQITC"
BASE COUNT          451 a          804 c          728 g          419 t
ORIGIN      
        1 cggatcctct atacccgcca cgtattcccg cagggcctcg aggttctgcg ggggcaccag
       61 gccgcgcccc ccggcgccca tggcggtgag caggtagcag aactcgccgc cggggtacat
      121 gacgcccttg ggcatgcccg tggtgccccc cgtgtagagc atgtacacgc tcgccggatc
      181 ccgcttctgc ctcgccatgg gctcggcggc gtcgagcgag ctttcgtagt caaccgaccc
      241 cgccagcaga tcctcggtcc cgtcatccac ctgcacatag agcttcacgc ggggaagctg
      301 atcgcggatc tcttcgatgc gcggggcgta acacccctgg taaaagaccg cctccgcgtc
      361 cgcgttgtcc agcaggtaaa ccagctcctc cgccttgtag cggtagttca cgttgatcgg
      421 acagccgctg agcttgaaga cgccgtactg agcttcgaga tactcgttgc agttgtggag
      481 atacaggccc accttcgtat ccgcgcccag gccgtgggcc tgaagcagac cggccacccg
      541 cgtagcacgg ccgtcgtaat ccgcccaggt gcgggtcacg ccctcgcaga tgagggccgt
      601 ccggtccgga accgtgtcgg cgatggcctc ccaggccgat gcgaaatgaa aatccatggc
      661 agacgccctc cctgtagaag aaatccggat tggcaatggg tcgtccgtcc tgacatcgcc
      721 aggccgaccg aattcgccaa ctgttcgcag aaatcgccaa caccacaggc ccgcccgtcg
      781 ccccttccac ccggcgaggc ggcattaagc ctggagatcg gggttgttcg gagttggcat
      841 ggcatttgaa tattcagtaa cgaggccaac ggaaacgacc cggcttcagc tgcaaatgaa
      901 cgcgagcaga caatgaaacg gcaaaaccag atgataatca aagacaatgc acccgcaggc
      961 ccgatcgcgc gcctgtctag gatcacctat ccccgggctt cggcggcaaa cctgatgctg
     1021 ctgagcgtgg tgatcgccgc catcggcgcg attgcctaca tcctgcaccc cgatgccgcc
     1081 cagccggcgc cgaacacctt ccacggcgcg gccctgctgg tcagcaactg gctgctgtgc
     1141 ggctgacgac agaaagactc cggagagatc ccagtggcaa attcgaactt gaacaacgtc
     1201 cgcagacgac tgcgtctcga cccgcgcaac ggctggattg ccggcgcctg cgccggcttc
     1261 gccaatcggt tacagatcga ccctgccatc gtgcgggtcg ttgtggtgat cgccgccctg
     1321 ttcttcccca agacggtgat cgcctgttac ctgctggcat ggctggtgct ggacgaagct
     1381 cgctgagccc accggcggtg agcccttcca tcagcaggtg atctggcagg gctcctccgg
     1441 cggcagccag tttggcgcgt cgtcggccag ctcgcgaaag tctatagcca gctccacgaa
     1501 acgcgtgtac atgcgcaccg actgccgctc ggccatgtgg ttgagaagcc aggcggcggt
     1561 ctcgcgcccc aggttctcgt tgttgtcgaa gatggcggtc agctcggccc cgaaataggc
     1621 ctcgcgatta gcggccagct ccgcgaagcg atccgcaaag gccggttcgg agcgggcctg
     1681 gagcagcttc atgaggtcac gctgccagcg ccgccgatac tccgcccaga gaacccgctc
     1741 gggaatgtag cgaaccgact gagccaccag atactcctgc tgcgccgggg tcaggcgacc
     1801 ggcaaagcgg gtgaagatgt cctccacctc ttcgcgccag cgggcctggg acgcttcgac
     1861 gctcttgccg agctcggtct tctcgatctc ccggttgctc tcctccagcc gtgccgggag
     1921 gccggccacc tgctcatcgg tcatggtctg catgatggcg accgcgaggg gcgcgccggt
     1981 ctcgacgatc tctttcgccc agcccatcat ggtgttttcc agcgcgagca gttcctgggc
     2041 atcgacgccg tccgaaagcg tcgtcggcac ggcctcgaag aagtccgcat agagcggcat
     2101 atgctccgtg cgatgccagt aatgaagccg ggcgaattcc tcgttgaaca ggttccgctg
     2161 agcgtcgttc atgttcacgt aatcgttcac gccccaacgc accagccggt cgaggttgtt
     2221 gtaggcaaac tgaacgctgc agccggaacc aagcaccagc accgcgagca gcgcaaacag
     2281 acggacgacc atgcgctcaa tcatagatga agttcagaat ccagcgcgcc agcagcgcgc
     2341 ggtcgatctc gttgtcgagc gaggccaaac cggccgcaag ggtctcgggc cccagaagcg
     2401 ct
//
LOCUS       DBNHQR010000298         5010 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_978591, whole genome shotgun sequence.
ACCESSION   DBNHQR010000298 DBNHQR010000000
VERSION     DBNHQR010000298.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5010)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_978591"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="AC16VR_06685"
     CDS             complement(<1..372)
                     /locus_tag="AC16VR_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012562.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010880.1"
                     /translation="MRPDDLAPWKSLTRLSADSIKQPLSDWLRSPERFARLSFEVGEL
                     LVDLSKQRLNEEIVDALLALAGAARLDRRIDDLFEGAQVNPTEQRPALHTALRAPLAS
                     RPAAYGEAVETTLTRMLDFAER"
     gene            complement(497..1210)
                     /locus_tag="AC16VR_06690"
     CDS             complement(497..1210)
                     /locus_tag="AC16VR_06690"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HIR6010881.1"
                     /translation="MTPPAQHSLAIETTAALGQKLEGKSLPRYIVIEGPIGVGKTTLA
                     NKLAEAFHYPILLEPAAQNPFLNSFYRDGRRNALPTQLFFLLHRAQQIANLDHADLLG
                     PTLVADFLMEKDDLFARMTLDEDEYALYRQIHETLDIDPPKPDLVIYLQAPVDVLRTR
                     IRDRGIDYERDIEAGYLESLARAYTEFFHFYDAAPLLVVNAASIDFANNAVHFESLLD
                     QILAMDGLRQYFNPNPTLL"
     gene            complement(1264..2532)
                     /gene="pcnB"
                     /locus_tag="AC16VR_06695"
     CDS             complement(1264..2532)
                     /gene="pcnB"
                     /locus_tag="AC16VR_06695"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0043633 - polyadenylation-dependent RNA
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HIR6010882.1"
                     /translation="MSEPVRLDATEHGLLRDQVDPEALDVTRRLSEAGYQALLVGGCV
                     RDLLLGAEPKDFDVATDATPEEVRQLFRRSRLVGRRFRIAHVRYGRKVIEVSTFRRSH
                     TEDEDERRHSEEGVILRDNLYGSLEEDAFRRDFTINALYYDPETEEILDYVGGLEDLE
                     RADLRFIGEAEVRLREDPVRLLRAVRFQAKLNFQLDESIETALPDAAERLSVIPSARL
                     FDEVAKLMMSGAAGAAWKAIAGTPLRAALFPGTPPDSRLVELAMANTDQRIASDLPVT
                     PGFLLAVLMWHDFQARCNALTDERKPAEARHQAASETLAAQQAVTSMPRRFSQFVRDV
                     WSLQPRLEARLPRQIDRIVQHPRFRAAYDFLVLRAESGEQATDSEPLDDAAQWWTTFQ
                     QADQATRADMTEARLAVQPPRKRRRRRRRG"
     gene            complement(2529..3848)
                     /locus_tag="AC16VR_06700"
     CDS             complement(2529..3848)
                     /locus_tag="AC16VR_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003095138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR6010883.1"
                     /translation="MYHVLLVDSGDRRETVRGVLENAGITVVGPESIEALRQIDARQY
                     RCLLAAVGPLFGTTEDAIDLAGRLPVVLLDPLGDARRAVAAMKRGAADYLVCPFTEEE
                     LLSAVERCRPTGAPQPRDPLNMVGASQPMKDLYNRIAQVAPTDSTVLIEGESGSGKEL
                     VARAIHAASRRNDAPLITLNCATVPAELIESELFGRPAEGQAASRHGLLEAANGGTLF
                     LDEIAELPPNVQARLLRVLQDGCLGLSSDGTPFTVDVRIIAATHQNLRQLIEADQFRE
                     DLYYRLNVVNLPIPPLRARGDDVLQLAESILRRTCKRLSRTCPGFTRSAQEAMQQYAW
                     PGNVRELENAVERAVILCSESAIEPHLLAIDLTPPASVEPPPSMNDRSSLEDYFVRFV
                     TAHQDQLTETELAEKLGISRKSLWERRQRLNIPRKKTRKRGPRRDST"
     gene            complement(3904..4761)
                     /gene="gluQRS"
                     /locus_tag="AC16VR_06705"
     CDS             complement(3904..4761)
                     /gene="gluQRS"
                     /locus_tag="AC16VR_06705"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009050893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HIR6010884.1"
                     /translation="MAGRFAPSPTGPLHLGSLLAATASYLDARAAGETWRLRFDDLDV
                     PRNDPGAIGSITATLRAHGLIWDGPIVHQSDNLTAYEEALNRLDRMGLLFYCTCSRRD
                     LRAEAVYPGTCRAFREPRRDSATRVLVDQVAIEFADRIQGPQRCRLDQTVGDFIVRRR
                     DGLVAYQLATAVDDGGEEVTSVVRGADLLDNTPRQIYLMQRLGLKPPRYAHIPVLLKP
                     DGSKLSKQTMAPPVDAHRAPENLLTAYGLLGMRPPSDAQRWEVERILSWGISHWDQEK
                     VPRRRSVEP"
     gene            complement(4797..>5010)
                     /locus_tag="AC16VR_06710"
     CDS             complement(4797..>5010)
                     /locus_tag="AC16VR_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HIR6010885.1"
                     /translation="FSIELRTRDRERKLIRKIDQTLDRLDQGDFGYCDTCGVEIGLRR
                     LEARPTANQCVDCKSLDEIKERQLHG"
BASE COUNT          789 a         1568 c         1738 g          915 t
ORIGIN      
        1 ccgctccgcg aagtcgagca tgcgggtcag ggtcgtctcg acggcctccc cataggcggc
       61 gggccggctc gccagcggtg cgcgcagggc ggtgtgcaaa gccggccgct gctctgtggg
      121 attgacctgt gcgccttcga acaggtcgtc tatccggcga tccagccggg cggccccggc
      181 cagcgccagc agggcatcga cgatctcttc gttgagccgc tgtttggaca agtcgacgag
      241 cagttctccg acctcgaagc tcagccgggc gaatctctcc ggcgaccgca accagtcgct
      301 caacggttgt ttgatgctgt ctgccgaaag ccgggtcagg ctcttccacg gcgcgaggtc
      361 gtcgggcctc atgggctggc ccccgaagcc ctggtgccgc ccggcaagat cggcctgctc
      421 gcggcaggcg ggccggggat gctgcgggcc tgtgcccctt tagcgctggc ggcggcggtg
      481 aatcccaacg gtgcgttcaa agcagggtgg gattcgggtt gaagtactga cgaaggccgt
      541 ccatcgcgag tatctggtcg agcagggact cgaagtgaac ggcgttgttg gcgaagtcta
      601 tggacgctgc gttgacgacc agcaggggcg cggcgtcata gaaatgaaag aactccgtat
      661 aggcccgggc caggctttcc aggtacccgg cctcgatgtc gcgttcgtag tcgatgccgc
      721 ggtcacgaat gcgcgttcgc agaacgtcca ccggggcctg caggtagatg acgaggtcgg
      781 gctttggcgg gtcgatgtcc agcgtctcgt ggatctggcg gtagagggcg tactcgtcct
      841 catccagggt catccgggcg aacaggtcgt ccttctccat gaggaaatcg gcgaccaggg
      901 tgggcccgag caggtcagcg tggtcgaggt tggcaatctg ctgcgccctg tggagcagga
      961 agaacagctg ggtgggcaac gcgttgcgcc ggccgtctcg gtagaagctg ttgagaaacg
     1021 ggttctgcgc ggcgggctcc aggagaatcg ggtagtgaaa ggcctcggcc agcttgttgg
     1081 caagggtggt cttgccgacc ccgatggggc cctcgatgac gatgtaacgc ggtagagatt
     1141 ttccttccag tttctgcccg agcgcggccg tagtttcgat ggctagagag tgctgggccg
     1201 gtggcgtcat ggagcggatc agctggtcgg atgccttaat aatggacaag gcatcccttc
     1261 gcttcaacca cggcggcgcc ttctcctccg ctttcttggt ggctggaccg ccagtcgcgc
     1321 ctcggtcata tcggcccgcg tggcctggtc tgcctgctgg aaggtagtcc accactgcgc
     1381 agcatcatcg agcggttcgc tgtcggtggc ctgctcgccc gactccgccc gcagcacgag
     1441 aaagtcgtag gcggcccgga atctggggtg ctgcacgatc cggtcgatct gcctcggtag
     1501 cctggcctcc agacgtggct gcaggctcca gacgtcgcgt acgaactgag agaaccggcg
     1561 cggcatggac gtaaccgcct gttgagcggc cagggtctcg gacgccgcct ggtgccgggc
     1621 ctcggcgggc tttcgctcgt cggtcagagc gttgcatcgg gcctggaagt cgtgccacat
     1681 gagcaccgcg agcagaaagc ccggggtgac gggcagatcg ctggcgatac gctggtcggt
     1741 attggccatg gccagctcga ccagccggga atcgggaggt gtgccgggga aaagcgcggc
     1801 ccgcagcggc gtgcccgcaa tggctttcca ggcggctccc gcagctccgg acatcatcag
     1861 cttggcgacc tcgtcgaaca gccgtgcgga gggtatgacg ctgagccgtt cggcggcatc
     1921 gggcagggcg gtctcgatgc tctcatcgag ctggaaattg agtttcgctt ggaacctcac
     1981 cgcgcgcagc agccgcacgg ggtcctcgcg cagccggacc tcggcctcgc cgatgaagcg
     2041 gaggtccgcc cgctccagat cctccagacc acccacgtag tccagaattt cctcggtttc
     2101 gggatcgtag tagagcgcgt ttatcgtgaa gtccctgcgg aaggcgtcct cttccaggct
     2161 gccgtacagg ttgtcgcgga gaatgacccc ttcctccgag tgacgacgct cgtcctcgtc
     2221 ttcggtgtga gagcggcgga aggtcgatac ctcgatgacc ttgcggccgt agcgcacgtg
     2281 agcaatgcga aatctgcggc cgaccagccg ggagcgtcga aagagctggc gaacctcctc
     2341 cggcgttgca tccgttgcca cgtcgaaatc ctttggctct gcgcccagga gcaggtctct
     2401 gacgcacccg cccacgagaa gggcctggta gccggcctcg gacagccgcc tggtgacatc
     2461 cagagcctcg ggatccacct ggtctctaag gagaccgtgc tccgtcgcgt ctagcctgac
     2521 cggctcgctc aagtgctgtc cctgcgcgga ccccgcttgc gggtcttctt acgaggaatg
     2581 ttcagccgct gacgcctctc ccacaggctc ttgcggctga tccccagctt ttccgcgagc
     2641 tcggtctccg ttagctggtc ctggtgggcg gtcacgaacc gcacgaaata gtcctccagg
     2701 ctggatcggt cgttcatgga gggcggcggc tcgacggaag cgggcggagt caggtcgatg
     2761 gccagcagat gcggttcgat tgcggactcc gagcacagga tgacggccct ttccacagcg
     2821 ttctcgagct ctcggacgtt gccgggccag gcgtactgct gcatggcttc ctgggccgat
     2881 cgggtgaatc ccgggcaggt cctcgagagt cgtttgcacg ttcgcctgag gatgctctct
     2941 gcgagctgta ggacatcgtc gcccctggct ctcaacggcg gaatcggcag attcacgacg
     3001 ttcaggcgat agtagagatc ctcccggaac tggtctgcct cgatgagctg tctcaggttc
     3061 tggtgggtcg cggcgatgat gcgcacgtcc acggtgaagg gggtaccgtc gctggacagg
     3121 cccaggcagc cgtcctgaag cactcgcagc agacgcgcct gaacgttcgg aggcagctcg
     3181 gcgatctcgt ccagaaacag ggtcccgccg ttggccgctt ccaaaagccc gtggcggctg
     3241 gccgcttgcc cctcggctgg tctgccgaac agctcggact cgatcagctc agccggcacg
     3301 gtggcgcagt tcagcgtgat cagcggcgca tcgttgcggc ggcttgccgc gtggatcgcg
     3361 cgcgctacga gctcctttcc ggagccggac tctccctcga tgagcaccgt tgagtcggtg
     3421 ggcgcgacct gggcgatccg gttgtacaga tccttcatcg gctggctcgc gcccaccatg
     3481 ttcagcgggt cacgcggctg gggggcgcct gtggggcggc agcgctctac ggcgctcagg
     3541 agctcctctt ccgtgaaggg gcagaccagg tagtcggcgg cgccgcgctt catggctgcc
     3601 accgcccggc gagcgtcgcc taggggatcc aggagcacca cggggagcct gccggccagg
     3661 tcgatggcgt cttcggtcgt tccgaacaga gggcccacag cggcgagcag gcaccggtac
     3721 tggcgggcat cgatctggcg gagcgcttcg atgctctccg gccctaccac cgtgatgccg
     3781 gcgttctcga gaacgcctcg aacggtctcg cgacgatcgc ccgaatccac cagaagtacg
     3841 tgatacatgg cgcgcgatgg taacaaaaag gtcagggtgc gcgcgcgacc ctgcagtggg
     3901 gcgtcaaggc tcgacggacc gccgacgtgg gaccttctcc tggtcccagt ggctgatgcc
     3961 ccaggagaga atgcgctcga cttcccaccg ttgcgcatcg gaaggcgggc gcataccgag
     4021 caggccgtac gccgtcagca ggttctcggg cgcccgatgg gcgtccacgg gcggggccat
     4081 ggtctgcttg gacagcttgc tgccgtcggg tttcaggagg acgggaatgt gcgcgtagcg
     4141 cggcggcttc agaccgagcc gctgcatcag gtagatctgg cggggcgtgt tgtccagcag
     4201 gtcggcgccc ctgaccaccg aagtaacctc ttcgcccccg tcatccaccg cggtggccag
     4261 ctggtaggcc accaggccgt cgcgtcggcg cacgatgaag tcgcccaccg tctggtcgag
     4321 acgacagcgt tgcgggcctt gtatgcggtc ggcgaactcg atggccacct ggtccaccag
     4381 caccctggtg gcgctgtctc gtcggggctc gcgaaaggcg cggcacgtgc cggggtaaac
     4441 cgcctcggcg cgcaggtcgc gcctggaaca ggtgcagtag aacagcagcc ccatgcggtc
     4501 gagacggttc agcgcctctt cgtatgccgt caggttgtcg ctctggtgga cgatgggccc
     4561 gtcccagatc aggccgtgag ccctgagcgt ggcagtgatg ctcccaatgg cgccgggatc
     4621 gtttctggga acgtccagat cgtcgaagcg cagccgccag gtttcgccgg cggcacgggc
     4681 atccaggtag ctggcggtcg cggcgagaag ggatccgagg tgcagcggac cggttggtga
     4741 cggcgcgaac cgaccggcca tgccttgcga agatgcgcgg gagacggagc gtcccgtcag
     4801 ccgtgcagct gcctctcctt gatctcgtcg agggacttgc agtcgacgca ctggttggcg
     4861 gtgggtcgtg cctccaagcg gcgcagaccg atctcgaccc cgcaggtgtc gcagtagccg
     4921 aagtcaccct ggtccaggcg atcgagggtc tggtcgatct tcctgattag cttgcgctcc
     4981 ctgtcgcggg tccggagctc tatgctgaat
//
LOCUS       DBNHQR010000299         2069 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1484333, whole genome shotgun sequence.
ACCESSION   DBNHQR010000299 DBNHQR010000000
VERSION     DBNHQR010000299.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1484333"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="AC16VR_06715"
     CDS             complement(<1..386)
                     /locus_tag="AC16VR_06715"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR6010886.1"
                     /translation="MRVVVIGAGIGGSALALALDRLGIEHVVLEQAPEFTDVGAGIQL
                     SPNGVRILESLGLRDALKGFCTTPDFHKYSVWDTGETILRTALMPRVREAYGFAYYHA
                     WRPDLIAALTNNLSAGVNMNRKVVRVG"
     gene            complement(383..1387)
                     /locus_tag="AC16VR_06720"
     CDS             complement(383..1387)
                     /locus_tag="AC16VR_06720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6010887.1"
                     /translation="MPELRVGVCYDFRNPPDSGVSDARLYAEVMEQVRWLDGIGADLV
                     WFTEHHFVDDGYLPSWTPVAGAMAAVTENVRFGTDICLMPFNHPVRLAEDLAVLDNLS
                     GGRVELGIGMGYAPHEFRGFGLPVSRRVSLMNEGIEVLQRCFTGERFSYHGKRYQLDD
                     VRITPGYVQPGGPPLWIAAMSEGGAARAARYEANFLPQGNRARVYDTWVDALESSGRS
                     PADHRIGIIRSILVTDDRETDWPVIRAAERYRMALYQRFFEESGEGFGDPREAIPQTW
                     IVGNVDQCVAELQQFISDFRITDIVTMAVPPGLRAEQMAPSLERLFTEVVPRLKGGRP
                     "
     gene            complement(1476..1889)
                     /locus_tag="AC16VR_06725"
     CDS             complement(1476..1889)
                     /locus_tag="AC16VR_06725"
                     /inference="COORDINATES: protein motif:HMM:NF013304.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HIR6010888.1"
                     /translation="MLDPTTITIFGLVLIKLLLVASIGMHRLYIASTSDKAPNTFLPT
                     GEDVGRFAVRLARAHANCYEFLPFALAVLVYAAATESSALTDGLAYYFLIARIGQSAT
                     HLVSTSTTAVFIRFAFFLAQVVILLVWCLGFLGAL"
BASE COUNT          369 a          664 c          701 g          335 t
ORIGIN      
        1 cccacgcgga cgaccttccg gttcatgttc accccggccg aaaggttgtt ggtcagcgcg
       61 gcgatgaggt ccgggcgcca ggcgtgatag taggcaaagc cgtacgcttc ccgtacccgg
      121 ggcatcagcg ccgtccgcag aatggtctcg ccggtgtccc agacgctgta cttgtggaaa
      181 tccggagtcg tgcagaagcc cttgagcgca tcgcgcaggc cgaggctttc caggatccga
      241 acgccgttgg ggctgagctg gatgcccgcg cccacgtcgg tgaactcggg cgcctgctcg
      301 agaaccacgt gctcgatacc gagccgatcc agtgccagcg ccagcgcgga gccgccgatg
      361 cccgcgccga tgacgacgac cctcatgggc gacctccctt caaacgagga accacctcgg
      421 tgaacagacg ctccaggctc ggcgccatct gctcggcgcg tagcccgggc gggactgcca
      481 tggtaacgat gtcggtgatc cggaaatcgc tgatgaactg ctgcagctcg gcgacgcact
      541 gatccacgtt gccgacgatc caggtctggg ggatggcttc ccgcgggtcg ccgaaaccct
      601 cgccgctttc ctcgaagaac cgctggtaca gggccatgcg atagcgttcg gcggcacgga
      661 tcacgggcca gtccgtttcc ctgtcgtcgg tcacgagaat gctgcggatg atgccgatcc
      721 gatgatcggc cgggctgcgg ccggagcttt ccagagcatc gacccacgtg tcgtagacgc
      781 gcgccctgtt cccctggggc aggaaattgg cttcgtaccg ggccgcccgc gccgcacctc
      841 cttcggacat ggcggcgatc cacaacggcg ggccgccggg ctgcacgtac cccggcgtga
      901 tacggacgtc gtcgagctgg tagcgcttgc cgtgatagct gaaacgctcg ccggtgaagc
      961 agcgttgcag cacctctatg ccctcgttca tcagggagac ccggcgtgat accggcaggc
     1021 cgaagccgcg gaactcgtgg ggagcgtagc ccatgcctat gccgagctcg acccgccccc
     1081 ccgacaggtt gtcgagcacg gccagatcct cggccaggcg gacgggatgg ttgaagggca
     1141 tcaggcagat gtcggtgccg aagcgcacgt tctccgtgac tgcagccatg gcgccggcca
     1201 ccggcgtcca gctgggcagg tagccgtcgt cgacgaagtg gtgctcggtg aaccagacca
     1261 ggtcggcgcc gataccgtcc agccagcgaa cctgctccat gacctcggcg tacagccgcg
     1321 cgtcgctcac tccggaatca ggcggattgc ggaagtcgta gcagacgccg actcgcagtt
     1381 caggcatggg aatatccggc tggggcatgg gcccgagcat agcatgcgtg ccatgccctc
     1441 aacgctgcgc ccggccggcg acgccttcgc cgagctcaga gcgcgccgag aaagcccagg
     1501 caccagacga gcagaatcac cacctgcgcc aggaaaaacg cgaagcggat gaacacggcc
     1561 gtcgtgctcg tcgaaaccag gtgcgtggcc gattgtccga tgcgtgcgat cagaaagtag
     1621 taggcgagcc cgtcggtgag cgcgctgctt tccgtggccg ccgcgtagac caggacggcc
     1681 agagcgaagg gcagaaactc gtagcagttg gcgtgggccc gggccagccg gacggcaaac
     1741 cggccgacgt cctcgccggt gggcagaaag gtgttgggcg ccttgtcaga cgtcgatgcg
     1801 atgtacaggc ggtgcatgcc gatgctggcg acgagcagca gcttgatcag aaccagaccg
     1861 aagatggtga tggtggtggg gtcgagcatt gaattctctc cttgccttgt tgtttcgtgt
     1921 gggaggcgtc gggcagatgt cggcgatcac tcctgggggt aacgccaggg ggtctgtggc
     1981 tgctcggcgg actcgtccag gcaccaaagc ggcagcgcga tgccgtcgcc cagctgtttg
     2041 aacactaccc gcacgcggct gccgatacc
//
LOCUS       DBNHQR010000300         3201 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_661575, whole genome shotgun sequence.
ACCESSION   DBNHQR010000300 DBNHQR010000000
VERSION     DBNHQR010000300.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_661575"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..995
                     /locus_tag="AC16VR_06730"
     CDS             <1..995
                     /locus_tag="AC16VR_06730"
                     /inference="COORDINATES: protein motif:HMM:NF013781.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HIR6010889.1"
                     /translation="SQMTRTVDPLGGSYYVESLTNSIVEAAREIIDEVEAMGGMTKAV
                     VAGMPKQRIEEASARRQARVERGEDVIVGVNKYKLEKDDSVFDVLAVDNSKVRDAQLK
                     RLARIRASRDEIATQAALDALTEAARTGEGNLLDLSVAATRLRATVGEVSTALEKVWN
                     RHTPVARMTPGVYADAHQEDEQFVRVRDEVKVFTELEGRPPSILVVKMGQDGHDRGAK
                     VISNAFGDLGFEVKMGPLFQTPAEAASQATDEAVNVVGVSTLAGGHKTLVPEMIEALK
                     AAGNEDVIVVVGGVIPQKDYQFLYDCGVHAIYGPGTNIPEAASDVLQLIPGKNR"
     gene            1024..1434
                     /gene="mce"
                     /locus_tag="AC16VR_06735"
     CDS             1024..1434
                     /gene="mce"
                     /locus_tag="AC16VR_06735"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HIR6010890.1"
                     /translation="MIGKLNHVAIVVPDLESAAALYRDTLGATVSDPVPLPDHGVTTV
                     FVELPNSKIELLHPLGDGSPVANYLERNPKGGMHHLCYEVQDIEAAIAHLVAEGATVL
                     GNGKPSTGAHGLPVVFLHPKDFCGTLIELEEVAA"
     gene            1487..2236
                     /locus_tag="AC16VR_06740"
     CDS             1487..2236
                     /locus_tag="AC16VR_06740"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR6010891.1"
                     /translation="MTTLVRIDSFDFPPGRKITARYQVVSQLGAGWESEVYLIRETAT
                     GIERTAKVFFPHRNRGNKVARRTAEKLHKLRDCPALIQYVAQETITVRRQAVTVLISE
                     YVEGDLLSEFIARQPGGRMHRFEALQLLRALALALEPIHERREYHGDLHAGNVIVRRV
                     GLDFDVRLIDVYHLDQRKSLSVKDDVCDAIRIFYDALGGQRFYARQSPEIKAICRGLK
                     RSLISERFRTAGQLRRYLDTMSWESEPRRGR"
     gene            2245..>3201
                     /locus_tag="AC16VR_06745"
     CDS             2245..>3201
                     /locus_tag="AC16VR_06745"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6010892.1"
                     /translation="MRGTIARLSALLTAVAILLIGHGLQLTLLPVHAEGLGWSASAIG
                     TSGSAYFLGFVVGCVLIPPVVGRVGHIRTFMVMGALATVALLAAGLLVTLPAWLIFRF
                     VTGFALSGLYMVIESWLVDVTQSNNRGTILALYTVICLLGMSVGQAFLGIASPMSLEL
                     FMAGAALICLAIVPVGLARVAAPHPIPSARFSPAVLLKASRVAMVCAFAGGLVTGAFW
                     TLGPVLARAFGLDAAQVGGMMGAGIIGGAAVQIPVGRLSDRTDRRLVIGGLMAAGALV
                     ALCGWFLAGEDSIALSVIMFLFGAATFCIYAVCIVHASDNADI"
BASE COUNT          539 a          997 c         1081 g          584 t
ORIGIN      
        1 agtcccagat gacccgcacg gtcgaccccc tgggcggcag ctattacgtg gaaagcctga
       61 ccaactccat cgtggaggcg gcccgtgaga tcatcgacga ggtcgaggcc atgggcggca
      121 tgaccaaggc ggtcgtggcc ggcatgccca agcagcgcat cgaggaggcc tcggcgcgcc
      181 gccaggcccg ggtcgagcgg ggcgaggacg taatcgtggg ggtgaacaag tacaagctcg
      241 aaaaggatga ctccgtcttc gacgttctcg cggtggacaa cagcaaggtg cgcgacgcgc
      301 agctcaagcg gctggcgcgc attcgcgcct cccgggacga gatcgccact caggccgccc
      361 tggacgcgct caccgaggcc gccaggacgg gcgagggcaa cctgctggat ctcagcgtgg
      421 cagcgacgcg cctgcgggca accgtcggcg aggtctcaac cgcgctggag aaggtctgga
      481 atcgccatac gcccgtagcg cgaatgaccc cgggggtgta tgccgacgcc catcaggagg
      541 acgagcagtt cgtgcgggtt cgcgacgagg tcaaggtgtt caccgagctc gaagggcggc
      601 cgccgagcat cctggtggtc aagatgggcc aggacggcca cgaccgcggg gccaaggtca
      661 tctccaatgc tttcggcgac cttggcttcg aagtgaagat ggggccgctg ttccagaccc
      721 cggcggaggc cgcgtctcag gcgaccgacg aggcggtgaa cgtcgtaggc gtctcgaccc
      781 tggctggagg acacaagacg ctggtgcccg agatgatcga agcgctcaaa gcggcgggta
      841 acgaggacgt gatcgtcgtg gtgggcgggg tgattccgca gaaggactat cagtttctct
      901 acgactgcgg cgtgcacgcc atctacgggc cgggcaccaa cataccggag gcggccagcg
      961 acgtgctgca gctgattccg ggcaagaacc gatgaacaac ctgaacacgg aacggaagag
     1021 caaatgatcg gtaagctgaa ccacgttgcc atcgtcgtcc cggacctcga atccgcggcg
     1081 gcactctaca gggacacgct gggcgccacg gtgtccgatc ccgtgccgct cccggatcac
     1141 ggcgtcacga cggtcttcgt cgagctgccc aacagcaaga tcgagctgct acatccgctg
     1201 ggtgacggat ctccggtggc gaactacctg gagcggaacc cgaagggcgg gatgcatcac
     1261 ctgtgctacg aggtccagga catcgaagcc gccatcgcgc acctcgtcgc ggagggcgcc
     1321 acggtgcttg ggaacggcaa gccgtcgacg ggggctcatg gcctgcccgt ggtctttctg
     1381 caccccaagg atttctgcgg cacgctgatc gagctcgaag aagttgctgc ctgaacgtgc
     1441 ccggatcgta gccagcccct gagcgcccga gaggcttgat gcagcggtga ccacccttgt
     1501 ccgcatcgac agcttcgatt ttccgccggg ccgcaagatc acggcccgat atcaggtcgt
     1561 cagccagctt ggagccggct gggaaagtga ggtctatctc atccgcgaga cggccaccgg
     1621 tatcgagcgg acggccaaag tgttctttcc gcaccgaaac cggggcaata aggtggcgcg
     1681 ccgcacggcg gaaaagctac acaagctcag ggactgcccg gcgctgatcc agtacgtggc
     1741 ccaggaaacg atcaccgtca ggcggcaggc cgtgacggtg ctcatctcgg agtacgtgga
     1801 gggcgatctg ctcagcgagt tcatcgcgcg gcagccgggt gggcgaatgc accgcttcga
     1861 ggcgctgcaa ctgctgcggg ccctggcgct ggcgctcgaa ccgattcacg agcgtcgcga
     1921 gtaccacggc gatctgcacg cgggcaatgt catcgtgcgt cgggtcggtc tcgatttcga
     1981 tgtccggctc atcgacgtct accacctcga tcagcgaaag tcgttgagcg taaaggacga
     2041 cgtctgcgat gccatccgga tcttttacga tgccctgggc ggccagcgtt tctacgcccg
     2101 gcaatcgcct gagatcaagg cgatctgtcg cggtctcaag cgttcgctca tctcggagcg
     2161 ctttcgcacc gctggtcagc tgcgccgtta cctggacacc atgtcctggg aatccgagcc
     2221 ccggcgaggg cggtaggcag aaccatgcgc ggaacgatcg cccgtctgag cgctctgctg
     2281 accgctgtcg ccatcctgct gatcggccac ggtctccagc ttacgctcct gccggtgcac
     2341 gcagagggtc tgggttggtc ggcttccgcc atcggtacca gcgggtcggc ctattttctg
     2401 ggtttcgtgg tgggctgcgt tctgattcca ccggtggtcg gcagggtcgg ccacattcgc
     2461 actttcatgg tgatgggggc cttggccacc gttgcgctgc tggctgccgg gttgctcgtg
     2521 accctgcctg cctggctcat cttccggttc gttaccgggt tcgcgctgtc cggtctctac
     2581 atggtgatcg aaagctggct ggtggacgtg acgcaatcga acaatcgggg aacgatactg
     2641 gctctctaca cggtcatctg cctgctgggg atgtccgtgg ggcaggcttt cctcggcata
     2701 gcctcaccca tgagcctgga gctcttcatg gcaggcgctg ccctcatctg cctggcgatc
     2761 gtgcccgtgg gtctggcgcg ggtggccgca ccgcacccca ttccctcggc tcgcttctcc
     2821 cccgccgtcc tgctgaaagc atcccgggtc gccatggtct gcgcgtttgc aggcggtctg
     2881 gtgacaggcg ctttctggac gctcggaccg gttttggcgc gagccttcgg cctggacgcc
     2941 gcgcaggtcg gcgggatgat gggtgcgggc atcattggag gtgccgcggt tcagataccc
     3001 gtcggccggc tctcggatcg taccgaccgg cgactggtga tcggcgggct catggcggcc
     3061 ggcgctctgg tggcgctgtg cggctggttc cttgctggtg aagactccat cgcgctgtcg
     3121 gtcatcatgt ttctctttgg tgcggcgacg ttctgcatat acgcggtctg catcgtgcac
     3181 gccagtgaca atgcggacat t
//
LOCUS       DBNHQR010000301         4648 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1104993, whole genome shotgun sequence.
ACCESSION   DBNHQR010000301 DBNHQR010000000
VERSION     DBNHQR010000301.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4648)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1104993"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="AC16VR_06750"
     CDS             <1..195
                     /locus_tag="AC16VR_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010893.1"
                     /translation="YAVKSETYEKAMKIARPVVNRVKQAEADTFGSDCPMAGRLIAHG
                     MEDDGAAEHPITMVRRAYGV"
     gene            199..780
                     /locus_tag="AC16VR_06755"
     CDS             199..780
                     /locus_tag="AC16VR_06755"
                     /inference="COORDINATES: protein motif:HMM:NF023432.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HIR6010894.1"
                     /translation="MQKLNRTDLWSLEQYSEERPAFRAEVIAHKKARRVAIGPHATLC
                     FEDALTMKYQIQEMLRVEKIFEAAEIEEELTTYNPLIPDGANWKATFLIEYPDVEERH
                     RALATMGGIEDTIWVQIGDGPRIYAIANEDMPRSEAGKAAAVHFLRFELDGASVQAAK
                     EGAPILAGIDHASLKGAVTLDPARAESLRRDLD"
     gene            complement(808..1692)
                     /gene="oxyR"
                     /locus_tag="AC16VR_06760"
     CDS             complement(808..1692)
                     /gene="oxyR"
                     /locus_tag="AC16VR_06760"
                     /inference="COORDINATES: protein motif:HMM:NF008361.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HIR6010895.1"
                     /translation="MNLRDLKYLVAVAERRHFGKAADACHVSQPTLSTQIKKLEEELG
                     VVLLERTNRQVMLTPVGERIVAQAQRVLREVDDLVHTAEQFRDPFGGEFRLGLIPTVA
                     PYLLPRILGPIRKSFPNLKIRLTEGQTAVVGERLRAGDLDAVILALPLEEENVVEAEL
                     YREPFYFAASKNHPKAGKREVALSDLDQEQVLLLEDGHCLRDQALEVCNSQNAVENTN
                     FRATSIETLRQMVAADVGITLMPELAIPQKHGAVRYIPFKGNGPHRRIGLCWRGSSSR
                     AALLHDMAGVLREVMAGG"
     gene            1758..2462
                     /locus_tag="AC16VR_06765"
     CDS             1758..2462
                     /locus_tag="AC16VR_06765"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR6010896.1"
                     /translation="MTPAIDVLHRSADLLVLNKPANVSLLADRSGEPCYWDALRQALG
                     DRTPYLVHRLDKGTSGVLLVALTRSAQRDLTRAFQQRAVRKFYVAWVVGSMALTSTHT
                     IDLPLQRGRKSRFRVAGPREQIRQQGTRWVLPATDTAGHPAITRIRTLANGHDRSLML
                     LAPRTGRSHQLRVHLSWIGYPIVGDHLYGRPTDPMQRADRLQLHCHRLVVPGFGTYRA
                     APGEGWLGRPDERPED"
     gene            complement(2464..2895)
                     /locus_tag="AC16VR_06770"
     CDS             complement(2464..2895)
                     /locus_tag="AC16VR_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010897.1"
                     /translation="MTRYLPGDIVARRKGLVMHKGVVTGDGRIFHNTPFRGEHVCTEA
                     EFRAGKRLYVQRQEFEARQRAVEHAERHGGSGYNLFTNNCEHTVYRATSGVGHSPQLK
                     SWIAGLGLATVAFAVTRHPGAAAAGYALGRRLAGRSADRRR"
     gene            complement(3085..3438)
                     /locus_tag="AC16VR_06775"
     CDS             complement(3085..3438)
                     /locus_tag="AC16VR_06775"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6010898.1"
                     /translation="MKRPLLALLVCCAFGCSESSNETPASTAAAGDGPAPAAHPGEET
                     YNRFCFSCHAAGIAGAPRTGDARAWASRVAKGEDQLLKSTIEGIAPGMPAMGLCRQCS
                     EDDLRAAIDYMIPDA"
     gene            complement(3508..>4648)
                     /locus_tag="AC16VR_06780"
     CDS             complement(3508..>4648)
                     /locus_tag="AC16VR_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011914120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HIR6010899.1"
                     /translation="LVSKLPDVQTTIFTVMSALAREEGALNLSQGFPDFDGPPELLER
                     VQYYLTHGHNQYAPMTGVAPLREATAGKIADLYGCQLDPEQEVTVTSGATEALFCAIA
                     AVVNAGDEVIVFDPAYDSYAPVVKLQGGVTRHVPLHPPDYRVDWDRVADNLSDRTRLI
                     IINTPHNPTGTVWGEDDIEALRAVIDGRDLFLLGDEVYEHIIFDGRQHASLCRYPDLF
                     ARSFVVSSFGKTYHTTGWKIGYCAAPAPLSAEFRRIHQFVTFTSITPVQLGLADFMTR
                     HPEHHLGLGAFYEAKRDLFCSLLEGSRFGLTKSAGTYFQLLDYGDYSNEADTDLAVRL
                     TREAKIASIPLSVFYADDPGHRMLRFCFAKDDATLRAGAEILLGL"
BASE COUNT          825 a         1516 c         1516 g          791 t
ORIGIN      
        1 tacgccgtca aaagtgaaac ctacgaaaag gccatgaaga tcgcccggcc cgtggtcaat
       61 cgggtcaagc aggccgaagc cgacactttc ggctctgact gccccatggc gggtcggctg
      121 atcgcgcacg gaatggagga tgatggggcc gccgagcatc cgatcaccat ggtccgtcgc
      181 gcctacggag tttgaaccat gcagaaactg aaccgcaccg atctgtggag cctggagcag
      241 tattccgagg agcgcccggc gttccgcgcg gaggtgatcg ctcacaaaaa ggcccgccgc
      301 gtagccatcg gcccccatgc cacgctgtgc ttcgaagacg ctctcaccat gaagtaccag
      361 atccaggaga tgcttcgcgt ggagaagatc ttcgaggccg cggagatcga ggaggagctg
      421 acaacctaca acccgctgat tcccgacggc gcaaactgga aggcgacttt tctcatcgag
      481 tacccggacg tggaggaacg gcaccgtgcg ctggccacca tgggcgggat cgaggacacc
      541 atctgggtgc agatcggcga cgggccgcgc atctacgcga tcgcgaacga ggacatgccg
      601 cgttcggaag cgggcaaggc ggcggccgtg cattttctcc gcttcgagct cgacggggct
      661 tccgtgcagg cggcgaagga aggcgcgccc atcctggcag gcatcgacca cgcgtcgctc
      721 aaaggagcgg tgaccctgga cccggccaga gcagagagcc tgaggcggga cctggactga
      781 accggacggc ccgccgcgcg ggcaccgtca gccgccggcc atgacttccc gaagaacgcc
      841 agccatgtca tgcagcaggg ccgcccggct ggagctgccc cgccagcaga ggccgattcg
      901 gcggtgcggc ccgttgcctt tgaacggaat gtatctcacg gcgccgtgct tctgcggtat
      961 ggccagctcc ggcatgagcg ttatgccaac gtccgccgcc accatctggc gcagcgtctc
     1021 gatgctggtc gcgcggaagt tcgtgttctc aacggcgttc tgagaattgc agacctcgag
     1081 agcctggtcg cgcaggcagt gcccgtcctc gagcagcaga acctgctcct gatcgagatc
     1141 gctgagcgct acttcccgct tgcccgcctt cggatggttc ttcgaagcgg caaaatagaa
     1201 cggctccctg tagagctcgg cttccaccac gttctcctct tccaggggca acgcgagtat
     1261 cacggcgtcc aggtcaccgg cacgcagccg ctcgccgacc accgccgtct gcccttcggt
     1321 cagccggatc ttgaggttgg ggaaggactt cctgatgggg ccgaggatcc tgggcagcag
     1381 gtagggcgcc acggttggaa tgaggcccag ccggaactct ccaccgaagg gatcccgaaa
     1441 ttgctccgcg gtgtggacca ggtcgtcgac ctcccgcagc acgcgctggg cctgggcgac
     1501 gatccgctct cccaccggcg tgagcatgac ctggcggttg gtccgctcga gcaggacgac
     1561 gccgagttcc tcctcgagct tcttgatctg ggtggacaga gtcggctggc tgacgtggca
     1621 ggcgtctgcc gccttgccga aatgccgtcg ctcggccacg gcaaccagat acttgaggtc
     1681 tctcagattc atggcgcgta tgttactttg ccgcggaggc cgcccactac ccagcagagc
     1741 cagcccgaag ccagccaatg accccggcaa tcgacgtact tcaccgcagc gccgacctgc
     1801 tagtcctcaa caagcctgcc aacgtgtctc tgctcgccga ccgaagcgga gaaccatgct
     1861 actgggacgc gctgaggcag gcgctcggcg accggacgcc ctacctggtc catcgcctgg
     1921 acaaaggaac gagcggggtg ctgctggtgg ccctgacccg gagcgcccag cgtgacctga
     1981 cccgcgcctt tcagcagcgg gccgtgcgca agttctatgt tgcctgggtg gtcggttcca
     2041 tggcgctgac atcgacccac accatcgacc tgcccttgca acgcggccgg aaaagccgtt
     2101 ttcgggtcgc cggcccgcgg gagcagatcc ggcagcaggg cacgcgctgg gtccttcccg
     2161 caacggatac ggcaggacat cccgccatca cgcggatccg cacgctcgcc aacggccacg
     2221 acagatctct catgctgctc gccccccgaa ccggtcgctc ccaccagctg cgcgtgcacc
     2281 tgtcctggat cggctacccc atcgtcggcg atcacctcta cgggcggccg acagatccca
     2341 tgcagcgcgc cgatcggctg cagctgcact gtcaccgtct ggtggttccg gggttcggca
     2401 cgtaccgggc cgccccaggg gagggctggc tgggacgccc ggacgagcgc cccgaggact
     2461 agatcagcgt cgccggtcgg ccgaccggcc ggccagccgg cggcccagcg catagccggc
     2521 ggccgctgcg ccggggtggc gggtgaccgc aaaggccacg gtcgcaagcc ccagcccggc
     2581 gatccacgac ttcagctggg ggctgtgacc gacgccggaa gtggcccggt acaccgtgtg
     2641 ctcgcagttg ttggtgaaca ggttgtagcc gctaccgcca tggcgctcgg cgtgctcgac
     2701 cgcgcgctgg cgcgcctcga actcctggcg ctgcacgtac agccgtttgc cggcgcgaaa
     2761 ttccgcctcg gtgcagacgt gctcgccccg gaacggggtg ttgtggaaga tccggccgtc
     2821 cccggtcacc acacccttgt gcatcaccag acccttgcgg cgggcaacga tgtctccagg
     2881 aagatagcgg gtcatgtgat taaggtcctg tcagttggcg gggggcaatc tccttgccac
     2941 ccgggtcgtt ctcatgattc gacaattcgt ctcggcattc gtttagcaac atccgttcca
     3001 ctattcaacc cattgatata attgcctttt ttgtcgatca agctgtggcc gggtggcgtt
     3061 tgcgccaaca tctggcgagg gcggctaagc atccgggatc atgtagtcga tggcggcgcg
     3121 caggtcgtcc tcggagcatt gcctgcacag ccccatggcg ggcatcccgg gcgctatgcc
     3181 ctcgatggtc gacttgagca gctggtcctc cccctttgcc acccgcgaag cccaggcacg
     3241 cgcgtcgccg gttcgaggcg ctccggcaat cccggcggcg tgacaggaaa agcagaagcg
     3301 gttgtaggtc tcttctcccg gatgtgcggc tggggccggc ccgtcacctg cggccgccgt
     3361 cgacgcgggc gtctcgttgg atgattccga gcagccaaat gcgcagcaga ccagcaatgc
     3421 cagaagtgga cgtttcaccc cgtttttctc ctcatgtcgg tcatcggatt catcccgttc
     3481 gcgcgcgctc atccggcggc gtcggcttca caggcctaag agtatctccg caccggctcg
     3541 caaggtagcg tcatccttgg caaagcagaa acgcagcatg cggtggccgg gatcgtcagc
     3601 atagaagacc gacaacggaa tggatgcgat cttcgcctcc cgggtgaggc gcacggccag
     3661 atccgtatcg gcctcgttgg agtagtcgcc gtagtcgagc agctggaagt aggtgccggc
     3721 actcttcgtc aggccgaaac gggagccttc cagcaggctg cagaacaaat cgcgcttggc
     3781 ctcgtagaac gcgccgagcc cgagatggtg ctccggatgc cgggtcatga aatcggcgag
     3841 accgagctga acgggcgtga tggaggtgaa ggtgacgaac tgatgaatcc ggcggaactc
     3901 ggcggaaagc ggcgcggggg ccgcgcagta gccgattttc cagcccgtgg tgtgataggt
     3961 cttgccgaac gacgagacca cgaagctgcg ggcgaaaaga tccgggtacc ggcacaggct
     4021 cgcgtgctgg cgcccgtcga aaatgatgtg ctcgtagacc tcgtcgccga gaagaaacag
     4081 gtcgcggccg tcgatcacgg cgcgcaacgc ctcgatgtcg tcctcgcccc agaccgttcc
     4141 ggtggggttg tgaggcgtgt tgatgatgat cagcctggtc cgatcgctca agttgtccgc
     4201 cacccgatcc cagtcgaccc gatagtccgg cggatgcaag ggaacgtggc gggtcacgcc
     4261 gccctggagc ttcaccacgg gcgcgtagct gtcgtaggcg ggatcgaaga cgatcacctc
     4321 gtcaccggca ttcaccaccg ccgctatggc gcagaagagc gcctcggtgg cgccggaggt
     4381 gacggtcact tcctgctccg gatccagctg gcagccgtaa agatccgcga tcttgcccgc
     4441 cgtggcttcg cgcaggggcg ccacgcccgt catcggcgcg tactggttgt gaccgtgggt
     4501 gaggtaatac tgaacccgtt cgaggagctc gggcgggcca tcgaaatcgg ggaaaccctg
     4561 ggaaaggttc agcgcgccct cctccctggc gagcgcggac atgaccgtga agatcgtggt
     4621 ctgcacgtcc ggcagcttgc tgacgaga
//
LOCUS       DBNHQR010000302         2197 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1295913, whole genome shotgun sequence.
ACCESSION   DBNHQR010000302 DBNHQR010000000
VERSION     DBNHQR010000302.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1295913"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1749)
                     /locus_tag="AC16VR_06785"
     CDS             complement(<1..1749)
                     /locus_tag="AC16VR_06785"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6010900.1"
                     /translation="MTRPLAWTIGLQLALMLLCSPARADDVRDLYFGEALFQAGQGKY
                     FEALERLDAELGQHYGLDEPELDTLHHHIREAEFFVGDFELNYRMHHRAGRAISAVLN
                     GDVDQSVRNEAAYRLARIHFQKAQMADALEALARIEGSMPARVRDDVEFLRANVYLAV
                     GRSAEAAEVLRELQGTKGLTGFSEFNLAVALLQDGRTAEALQQLDRAGQVDARDAMTH
                     SIRDKANLVLGGMLLESGRFEEARAPLERVRLEGPMSNEALLGAGWAELSGENFERAV
                     VPWSILADRDPTDAAVQEAKLALPYAYSRLNVHGRAAVLYGKALDTFGRELNKLDASM
                     GSIENGSFLEALVREEIHQDKDWVVRMRSLPETPETFYLVELLASHDFQTALQNYLDL
                     EDLRKKLATWQASFDAFDSVIAARADYFEPLLPEVDQTFRELDSRFRVRVEQYELLVK
                     RMQDLLVMPRPEFLATTEEKVLSTGLTAMDEALGGVGRDGNDELRQRIRRLEGIITWT
                     LHTQYDDRLTRFYENLQDMQGSVALLKEHYQAFVRTRQAATLSYQGYDAPIKRLRGRI
                     HQASAKVDQLMARQGRM"
     gene            complement(1752..>2197)
                     /locus_tag="AC16VR_06790"
     CDS             complement(1752..>2197)
                     /locus_tag="AC16VR_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010901.1"
                     /translation="EMRSLDEQVQEIKSDVLSIAAELDQLEERLLFPSNTQLTVFVAI
                     GDGTESFRLDSIRLGINDALATHHIYSFKELEALQQGGVQRLYMGNLPIGTHALEVSV
                     LGKLPSGKDFAHAERFTFDKGIDPALIGITVDPGSGKAPIALKDW"
BASE COUNT          365 a          688 c          737 g          407 t
ORIGIN      
        1 catccggccc tgacgggcca tgagctggtc cacctttgcg gaagcctgat gaatccgccc
       61 gcgaaggcgc ttgatgggcg cgtcgtatcc ctggtagctg agcgttgcgg cctggcgggt
      121 gcggacgaac gcctggtagt gttctttgag cagggcgacg gatccctgca tgtcctggag
      181 attctcgtag aagcgggtca gccgatcgtc gtactgcgtg tgcagcgtcc aggtgatgat
      241 cccttccaga cggcggatgc gctgacgtag ctcgtcgttc ccgtcgcggc ccaccccgcc
      301 aagagcctcg tccatggcgg tcagacccgt gctcaggacc ttctcctcgg tggtggccag
      361 aaactcggga cggggcatga ccaggaggtc ctgcatgcgt ttcaccagca gctcgtactg
      421 ctccacgcga acgcgaaagc gggagtcgag ctcgcggaac gtctggtcga cctcggggag
      481 caggggctcg aagtagtcgg cccgggcggc tatgacgctg tcgaaggcgt cgaagctcgc
      541 ctgccaggtg gcgagtttct ttcgcaggtc ctccagatcc aggtagttct gcagagcggt
      601 ctggaaatcg tgggaggcga gcagctctac caggtagaag gtctcggggg tctcgggcag
      661 gctgcgcatg cgaaccaccc aatccttgtc ctggtggatc tcctcgcgca cgagcgcctc
      721 gaggaacgac ccgttctcga tgctacccat ggaggcgtcg agcttgttga gctcccggcc
      781 gaaggtgtcg agcgccttcc cgtagagaac ggccgctcgt ccgtgaacgt tcaggcggct
      841 gtaggcatat ggcagtgcca gcttcgcttc ctgcacggcc gcgtccgtcg ggtcgcgatc
      901 ggccagaatg gaccagggca ccacagcgcg ctcgaagttc tcgccggata gctcggccca
      961 gccggcccct aagagcgcct cgttggacat gggcccttcc aggcgaacgc gctccagagg
     1021 cgcccgggcc tcctcgaacc gccccgactc cagcagcatg ccgccgagca ccagattcgc
     1081 cttgtctcga atggagtggg tcatggcgtc cctggcgtcg acctgacctg cccggtcgag
     1141 ctgctgcagg gcctcggccg tgcggccgtc ctgaagcaga gcaaccgcca ggttgaactc
     1201 ggaaaagccg gtgagcccct tcgttccctg cagctcccgg agcacctcgg cggcttcggc
     1261 cgagcggccg acggcgaggt agacgttggc gcggagaaac tccacgtcgt ctcgaacgcg
     1321 cgcaggcatg gatccctcga tgcgcgcaag cgcttccagg gcatcggcca tctgggcttt
     1381 ctgaaagtga atgcgtgcca gcctgtaggc ggcttcgttg cgcacggact gatccacgtc
     1441 gccgttcaag accgcgctga tggcgcggcc cgcgcgatga tgcatgcggt agttgagctc
     1501 gaagtcgccg acgaagaact cggcttccct gatgtggtgg tgcagggtgt ccagttccgg
     1561 ctcgtcgaga ccgtagtgct ggccgagttc cgcgtcgagt cgttccagcg cttcgaagta
     1621 cttgccttga ccggcctgga acaacgcctc gccaaaatac aggtcgcgga cgtcatcggc
     1681 gcgcgccggg ctgcaaagca gcatcaaggc cagctgcaac ccgatggtcc aggcgagggg
     1741 tctggtcatg gctaccagtc tttcagggcg atcggggcct tgccggagcc cgggtcaacg
     1801 gtgatgccga tcagagccgg gtctatgccc ttgtcgaagg tgaagcgctc ggcgtgggca
     1861 aagtccttgc cgctcggcag cttgcccagc acggaaacct ccagcgcgtg agtgccgatg
     1921 ggcaggttgc ccatgtagag acgctgaacg cctccctgct gcagcgcctc gagttccttg
     1981 aagctgtaga tgtggtgagt ggcgagcgca tcgttgatgc cgagccggat cgagtcgagc
     2041 cggaaagact ccgtcccgtc gccgatggcg acgaagacgg tcagctgggt gttggacgga
     2101 aagagcagcc tctcctcgag ctggtcgagt tccgcggcga tgctcagcac gtcggatttg
     2161 atttcctgaa cctgctcgtc gaggctgcgc atctcct
//
LOCUS       DBNHQR010000303         3643 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1737895, whole genome shotgun sequence.
ACCESSION   DBNHQR010000303 DBNHQR010000000
VERSION     DBNHQR010000303.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1737895"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..820)
                     /locus_tag="AC16VR_06795"
     CDS             complement(53..820)
                     /locus_tag="AC16VR_06795"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6010902.1"
                     /translation="MIARRNSQRPNSAELLEFGEYVKVLEASGEREDVVAFFDLDRTI
                     IAGYSITALMIERLRSGMSPRHLVAQVSRFISYGFGRAGYQELLEGTVAELVGLPEQE
                     LVELGERAYQRRLQRLIYPEARRLIGAHKELGHEVVMVTSATRYQAQPVARELAVDQI
                     CCTELEIRQGRITGRVSPCFGASKRAAAVHLGSARACSLADAYFYTDSCDDLPLLEAV
                     GRPVAANPKASLSEVATSRGWPQLAFRRQGEAGSVAA"
     gene            complement(932..1900)
                     /locus_tag="AC16VR_06800"
     CDS             complement(932..1900)
                     /locus_tag="AC16VR_06800"
                     /inference="COORDINATES: protein motif:HMM:NF025598.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HIR6010903.1"
                     /translation="MSARIGWLAALLAVQLLLTGALLLLEGRGADSSPAAFVEVAPDS
                     VDRVVVGGEETELEFTRAGEQWSLGSGAPADAEKIGEVLEKLTDIEPGWPVAATEASR
                     ERFRVTGDDHERRVALFAGEGKLAEIYLGTSPGFRRIHARRADSEEVYSIDFAVYELP
                     LSDSDWLDKDLLGADGEIASIIREEHWSLERGQEGWLLNLVPGMQDAEAGPGIGGADQ
                     DAAGRLVERVAELRVTGFAPLGPAYEPAAVFTVTDASGSYGLRIFKDPENDEYAVESD
                     RLSGRFGLAAYVAEQLVVEHADLRAAEPQDEAPAPGSEGFSDAGPG"
     gene            complement(1897..>3643)
                     /locus_tag="AC16VR_06805"
     CDS             complement(1897..>3643)
                     /locus_tag="AC16VR_06805"
                     /inference="COORDINATES: protein motif:HMM:NF021351.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HIR6010904.1"
                     /translation="GDEYEVLGFRDLIEVKVVGEADLDVQLRNPEFDLTRAIKKVLYG
                     FQGGGSLFANIQDPVRFVGYVSSADRLPPALADYRAVLVEALDALARESGGRFTSEFI
                     DPDAGDGSVAAQIAVDFGFQPMAASLFDSNVFYFYLTLGDANTLIQLPLPEAFSVEAT
                     RRVLEEGLKRFASGLLKTVALAAPEAPPYMGMGQPPQGNQYEQLKDVLRSDFEVETTD
                     LAEGVVPDSADLLMIVDPEGFNRRQLFAMDQFLMKGGTVVVATAPFSASLSQQSLMAA
                     PRTSGLEAWLEHMGVTLEPKLVMDAQNAAFPAPVTRQVGGFSFQELVMLDYPYFVDVR
                     GEGMNAESPIVAGLPQVTLTWASPIEIDAEQQEGRTVTELLTSSPRSWLSADTNVMPR
                     LDEQGLSGFAPEGAQAPRLLGALIEGRFESFFAGQTSPLLEPADGDDDNDAGADGDDE
                     TDPAATVGVVSSVIARSADSARLFLFSSDGFLTDQNLRMLGSAEGTIYTNTVQMMVNV
                     VDWSLEDQSLLSIRSRGHFNRTLPPLVDEEQLFWEYLNYGLALLGIGVVFLVHRGRKR
                     RSDRMYASWLAGGAS"
BASE COUNT          660 a         1251 c         1139 g          593 t
ORIGIN      
        1 aatcgagatc ttgcggccgt tgggtgtcgg ccaggtgtaa aggtcgatca cctcaggcgg
       61 ccactgagcc cgcctcgccc tgcctgcgga acgcgagctg cggccagccg cgcgacgttg
      121 cgacctcgga aagcgaagcc ttggggttgg cagccacggg ccgtccgacg gcttcgagaa
      181 gcggcaggtc gtcacaggaa tcggtgtaga aataggcgtc cgccaggctg caggcccgag
      241 cggaccccaa gtgaacggct gccgcccgtt tgctcgcacc gaaacacggg ctgaccctgc
      301 cggtgatcct gccctggcgg atttccagct ccgtgcagca gatctggtcg acggccagct
      361 cccgggcgac gggctgcgcc tgataccggg tagccgaagt gaccatgacg acttcgtgac
      421 ccagctcctt gtgcgcgccg atcaggcggc gcgcctccgg gtagatcagc ctctgcagac
      481 ggcgctggta ggcccgctcc cccagctcca ccagctcctg ctccggcagc cctacgagct
      541 cggccacggt tccttcgagc agttcctggt aaccggcccg gccaaagcca taggagatga
      601 agcgactgac ctgagcgacg agatgccgtg gcgacatgcc gcttcgaagc cgctcgatca
      661 tgagcgcggt gatggaatag ccggcgatga tggttcgatc cagatcgaaa aacgcgacca
      721 cgtcctcgcg ctcgccagac gcctccagaa ctttgacgta ctcgccgaac tccagcagct
      781 ccgcggagtt cggcctttga ctgtttcgcc gggcaatcat ggcaggccct ggtcgccgtt
      841 ggtcggttta gctctcacgc gccgcgccag cgcggtgcgc tccgcacgtc ggcgacgcct
      901 gaacaatgag accatcgaaa cacgagtcga ttcaaccggg gcccgcatcg gaaaaccctt
      961 cggacccggg cgccggcgct tcgtcctgcg gttcggcggc acgcaggtcg gcatgctcta
     1021 caaccagctg ctcggctacg taagcggcca gaccgaagcg gccggacaga cgatcggatt
     1081 ccacggcgta ctcgtcgttc tccggatcct tgaaaatccg cagcccgtaa gacccggatg
     1141 cgtccgtgac ggtgaagacc gcagcaggct cgtacgcggg gcccaacggc gcaaatccgg
     1201 tcacccgaag ctcggcgacc cgctcgacga gccggcccgc ggcgtcctga tcggcgccgc
     1261 cgattccggg gccggcttcg gcatcctgca tccccggcac caggttcagg agccagcctt
     1321 cctgaccacg ctccagggac cagtgttcct cccggatgat cgaggcgatc tccccatctg
     1381 cccccagcag gtccttgtcg agccaatccg aatcgctcaa cggcagctcg tagacggcga
     1441 aatcgatgct gtatacctcc tcggaatccg cccgtcgggc atgaatgcgg cggaatcccg
     1501 gcgacgttcc cagatagatc tcggccagct ttccttcccc cgcgaagagg gccacccgtc
     1561 gctcgtgatc atcgccggta acgcgaaacc gctcccgcga cgcttcggtt gccgccacgg
     1621 gccagccggg ctcgatgtcg gtgagttttt ccagaacctc gccgatcttc tccgcgtcgg
     1681 caggggcgcc ggagcccaac gaccattgtt cccccgctcg ggtgaactcc agctcggttt
     1741 cctcaccgcc gacgaccacc cggtccacgc tgtccggggc gacctccaca aacgccgccg
     1801 gcgagctgtc cgcgccacga ccttcgagga gcagtagggc gccggtcagc aggagctgaa
     1861 cggcgagcag cgcggcaagc cagccgatac gcgcgctcat gaggcacctc ccgccagcca
     1921 gctcgcgtac atccggtcgg accggcgttt cctgccccgg tggacgagaa agaccacgcc
     1981 gatgcccagc agcgcaagcc cgtagttgag gtactcccaa aacagctgtt cctcgtcgac
     2041 gagcggcggc agcgtccggt tgaagtgtcc gcgtgagcgg atgctcaaca ggctctgatc
     2101 ttctaaggac cagtccacga cgttgaccat catctgcacc gtgttggtat agatggttcc
     2161 ctccgcagag cccagcatgc gaaggttctg gtcggtcagg aagccgtcgg aggagaacaa
     2221 gaacagtcgg gcagagtccg ccgagcgggc tatgacgctg ctcaccaccc cgaccgtggc
     2281 cgccgggtcg gtctcgtcgt caccgtccgc ccccgcgtcg ttgtcatcgt ccccgtcagc
     2341 aggttcgaga aggggcgatg tctggccggc aaagaaagac tcgaagcgcc cttctatcaa
     2401 cgcgccgagc agccgcggcg cctgggcacc ttccggggca aacccggaga gcccctgctc
     2461 gtcgagtctc ggcatgacgt tggtgtctgc cgacagccag ctgcgcggtg agctggtgag
     2521 aagctcggtg accgttcgcc cctcctgctg ctcggcatca atctcgatgg gcgaggccca
     2581 ggtgagcgta acctgaggaa gcccggccac gatgggcgac tcggcattca ttccttcacc
     2641 gcgcacgtcc acgaagtacg ggtagtcgag catcaccagc tcctgaaagc tgaacccgcc
     2701 aacctgtcgc gtcaccggcg ccgggaacgc cgcgttctga gcatccatta ccagcttggg
     2761 ctcgagcgtc acccccatgt gctcgagcca cgcttccagg cccgacgtgc gcggcgccgc
     2821 catcaggctc tgctgggaca gagacgcgga aaagggcgcc gtggcgacca ccaccgtgcc
     2881 gcctttcatc agaaactgat ccatcgcaaa gagctgtcgc cggttgaacc cttcaggatc
     2941 cacgatcatc aggagatcgg cgctgtcggg cacgacaccc tcggccaggt ccgtggtctc
     3001 cacttcgaaa tcgctgcgca ggacgtcctt gagctgctcg tactggttgc cttggggtgg
     3061 ctggcccata cccatgtacg gcggcgcttc gggagccgcc agggccacgg tcttcagcaa
     3121 cccgctggca aagcgcttga gcccctcctc gagcacccgc cgggtcgctt cgaccgagaa
     3181 cgcctccggc agcggaagct ggatgagcgt gtttgcgtcg cccagggtca ggtagaaata
     3241 gaacacgttg gaatcgaaaa ggctggcggc catgggctgg aaaccgaagt ccacggcaat
     3301 ctgcgcggcc accgagccat caccagcgtc cgggtcgatg aactccgaag tgaaccggcc
     3361 gccggactcc cgggcaagcg catccagggc ctccacgaga acggcccggt agtcggccaa
     3421 tgcgggtggc agccggtcgg cgctggagac gtagcctacg aagcgcacgg gatcctgaat
     3481 gttggcgaag aggctgccgc cgccctgaaa gccgtaaagg accttcttga tggcgcgggt
     3541 cagatcgaac tcggggtttc taagctgaac gtcgaggtcg gcctcgccca ccaccttgac
     3601 ctcgatgaga tcgcggaagc cgagcacctc gtactcgtcg ccg
//
LOCUS       DBNHQR010000304         6971 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_535151, whole genome shotgun sequence.
ACCESSION   DBNHQR010000304 DBNHQR010000000
VERSION     DBNHQR010000304.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6971)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6971)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6971
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_535151"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="AC16VR_06810"
     CDS             complement(<1..830)
                     /locus_tag="AC16VR_06810"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR6010905.1"
                     /translation="MIFRLMSMACIGALAMSATSGFAAEAEGQRQARTGGIEEIIVTA
                     RKREETLQDAPLTVTALSKERIDSYDITSLERIAATTPNLYVGRVSNGSGAQITMRGI
                     GATGATSIGIEQSVAVILNGAYYGQGRVLNEGMFDLQQIEVLKGPQSLFFGKNATAGV
                     ISLTSAKPTEEFEATARVGYEFEAEQMRYEGIISGPISDTVGGRLAVRYSDMNGGYYK
                     NRAEQRNYVLTEYLPEYGAPAGFLPILDIDPPEGTIINDISPADLRQSPQEEELILRG
                     T"
     gene            complement(1014..1835)
                     /locus_tag="AC16VR_06815"
     CDS             complement(1014..1835)
                     /locus_tag="AC16VR_06815"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6010906.1"
                     /translation="MGRLEGKRAVIFGASMDGNIGQVMARRFRDEGAQVLVASRNAAA
                     SAAFAESIGGYSAACDITRRDDVFALASHAKDALGGVDVAVNSTGLAMGGPFLEFGED
                     DLDTMIALQFKGSFFFLQAMVGAMLDKPEPGGSIIQISSAVAHRSTTVDLGFDAYMGT
                     KAGIDHVVRCVANQFGRQGIRANSIAAGHTDTPMHHDAYGGDIPDWMKAAFSDATPMG
                     RYGTCDDVAAGAVWLASDECFMTGDALQINGGLTLRRNPLKEDLDRYEREWAGRG"
     gene            2014..3309
                     /locus_tag="AC16VR_06820"
     CDS             2014..3309
                     /locus_tag="AC16VR_06820"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6010907.1"
                     /translation="MPPVFELESPRFLGDEDIPFERYTSQEFFNREMERMWTRTWQWA
                     CREEHVADPGDYYVYEIGPHSVLIVRDALGEVRAYVNACLHRGTKFRSGGSDGAFSEI
                     RCPFHGWTWELDGSLKRVPYAWDAPHVSEREFSLPRVAVGLWGGFVFVNLDPDPPPFD
                     ECLAPIPEHLENFDLSKRFVELHIEKELNCNWKAAIEAFIENYHTQETHPQLLYGSDD
                     EGTQYDVFTDHVSRFFCAFGVPSPSIDPPPTEQELVDMMLVGDRSVLGDAVTVKPGET
                     ARIVMARVLRQTLGEKYAADLGRYTDTEMIDVSQYGLFPNMVLFPQLSLPMVYRFRPI
                     GENPDRTLFELLILRPAPDQGRPPAPARAHRLEERDSFTTVPGFDTSLGEVYDQDTGN
                     LRAQQEGFRASRKHGATLLNHQEVRVRHLHQTLDDYLQQ"
     gene            complement(3317..4762)
                     /locus_tag="AC16VR_06825"
     CDS             complement(3317..4762)
                     /locus_tag="AC16VR_06825"
                     /EC_number="1.13.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF015043.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006720 - isoprenoid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HIR6010908.1"
                     /translation="MSTVMRGANEPPTCHGGLENLDEEHDYWIDDIDGTIPADLTGTF
                     MRNGPGRQKIGGKPFGHWFDGDGMISQFTFTDSKAHFRNRYVRTPKYVEETEAQEILY
                     RGFGTQIPGGWTKNIFKWPANPANTSIIHHGGRLLALNEGGRPWELEPGSLETVGEFD
                     YDGALAGKAFSAHGKVHPQTGDYFNFGAGTTGTVRKSPCLNMYRINPQGRMAKTAQLP
                     LQTFPFCHDFALAGRHAVYFVNSIVFGGMGGFFLGRHSMSDGVRFDDDIPMRIIVVDL
                     DTLEVVREFETDPGAIIHFGNAFESGDEVVVDAMFQDNFEVNATLADVFNPSGRFGGG
                     YYNRYRLNLADGTMSCARVSECEAEFPVFNPSKAGQPHSVTWTACSVENGANSFFNGF
                     QRVTFDGDADVVTLPAGCYGSEPMFAAAEGATREDDGYLLEVVYDGYSHRSELQVYRA
                     DDVNERVCTLHLSHHLPHQFHGYFSPRTFLA"
     gene            complement(4764..5753)
                     /locus_tag="AC16VR_06830"
     CDS             complement(4764..5753)
                     /locus_tag="AC16VR_06830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIR6010909.1"
                     /translation="MASRPEVEVEPGNFELVEEPVSGLEPGTLLLKTRYLTVNPPMRM
                     ALVSGGIAGKPIPIGRTMYGSGLAEVVESRNPAFAAGDLVMGDLGWQEYVVSDGARRR
                     GVRKVAVPERLSPTTLMHVLGGSGHTAYFGMIEFCKPRVGDTLVVSTAAGSVGALVCQ
                     LGRLQGCRVIGIAGSDEKCEWVVDELGAEACINYRTEDVAARLAETCPNGIDVYFDNV
                     GGETLDAVLGQIAMGARVVLCGGTSQYNNDLDWYGPKNYFNLVYKQAEMSGYFVSNFA
                     HRFDESLARLTTLVRDGRLKYREDVLEGIESVPSALIRILRGENFGIPLIKVS"
     gene            complement(5840..6292)
                     /locus_tag="AC16VR_06835"
     CDS             complement(5840..6292)
                     /locus_tag="AC16VR_06835"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR6010910.1"
                     /translation="MSNHTACVEWTMGEGDFVSGRYSREHRWTFDGGAVVQASPTPAV
                     VPEPFSNPACVDPEEAFVAAVASCHMLTFLHIAGRAGFSVIGYRDEAVGTMTKNAQGA
                     YWISEITLNPAIEYAGNAPTEAELDDLHDQAHEHCFIANSIRTEVLVG"
     gene            complement(6356..>6971)
                     /locus_tag="AC16VR_06840"
     CDS             complement(6356..>6971)
                     /locus_tag="AC16VR_06840"
                     /inference="COORDINATES: protein motif:HMM:NF016705.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SOUL family heme-binding protein"
                     /protein_id="HIR6010911.1"
                     /translation="WGTTLEQPEYAVISKGDGYEVRRYEGYLVAQTRVSGEFDQAGTR
                     AFPVLAGYIFGNNVERREMAMTTPVVSYDADTSSGSTMAMTAPVFSEPAGAEDEASVD
                     PSQEKLWTYQFVMERKYSEASLPAPVDPRVEIKRIPPRLVAARRFSGRWLEENVKAAE
                     TALLSSVRAGGLEPVGQVVLARYNDPFTPWFLRRNEVLVEIAGD"
BASE COUNT         1250 a         2322 c         2105 g         1294 t
ORIGIN      
        1 gtgccccgaa ggatgagctc ttcttcctgc ggcgattgcc tcaagtccgc cggcgagatg
       61 tcgttgatga tcgtgccttc cggtggatcg atatcgagaa tcggtaagaa ccccgccggc
      121 gcgccatact cgggcaggta ctcggtcagc acataatttc tctgctcggc ccggttcttg
      181 taatagccgc cgttcatgtc cgaatagcgc acggccagcc ggccgcccac ggtgtcgctg
      241 atgggaccgg agatgatccc ttcatagcgc atctgctccg cctcgaactc gtaccccacc
      301 ctggcggtcg cctcgaattc ctccgtgggc ttcgccgagg tcagggagat cacgccggcc
      361 gtcgcgttct tgccgaaaaa cagcgactgc ggccccttga gaacctcgat ctgctgaaga
      421 tcgaacattc cctcgttcag gacccggccc tggccgtagt acgcgccgtt cagaatgacg
      481 gccaccgact gctcgatgcc gatgctggtg gcgccggtcg cgccgattcc gcgcatggtg
      541 atctgcgcgc cggaaccgtt ggagacgcgc cccacgtaga gattcggcgt cgtggccgca
      601 attctctcca ggctggtgat gtcgtagctg tcgatgcgct ccttcgacaa cgcggtcacg
      661 gtcagcgggg cgtcctgcag cgtctcttcg cgcttccggg ccgtgacgat gatctcctcg
      721 atacctcctg tgcgggcctg tcgttgacct tccgcttcag cagcgaaccc actggtggcg
      781 gacattgcca gggcgccaat gcacgccatg ctcatcagtc tgaaaatcac gattctctcc
      841 ccttttaatt ctcgacttgt ttctcgactc tatcgctgga ccggccaatc gtgcgccttc
      901 gaagcgtcgc cgcatcatcg ccttaccgcc ccgcgcgcac atcggccgcc ggacgttaca
      961 cgatgtcatt ccggcagaca aactgacgcc ggccgccacc gcgaaacggc cgctcagccg
     1021 cgcccggccc actcacgctc gtagcggtca agatcttcct tgagggggtt gcggcgcagg
     1081 gtgaggccgc cgttgatctg cagagcatct cccgtcatga agcactcgtc gctggcgagc
     1141 cagacggcgc cggcggcaac gtcgtcacag gtgccgtatc ggcccatggg ggtcgcatcg
     1201 gaaaacgcgg ccttcatcca gtccgggatg tcgccaccgt aggcgtcatg atgcatgggc
     1261 gtgtccgtat gaccggccgc tatgctgttc gcccttatgc cctggcgacc gaactgattg
     1321 gccacgcaac ggaccacgtg atcgatcccg gccttagtgc ccatgtaggc atcaaaaccg
     1381 agatcgacgg tcgtggagcg atgagcaact gcagagctga tctggatgat cgatccccca
     1441 ggctccggct tgtcgagcat ggcgcccacc atggcctgca ggaagaagaa cgagcctttg
     1501 aactgcagcg cgatcatggt atccagatcg tcttcaccga actcgaggaa cggcccgccc
     1561 atagccaggc cggtggagtt gacggccacg tcgacgccac ccagcgcgtc tttggcgtgg
     1621 ctggccagag cgaagacgtc gtcgcgtctg gtgatgtcgc atgccgccga gtagccgccg
     1681 atggattccg cgaatgcggc cgacgctgcg gcgttgcggc tggccaccag cacctgggca
     1741 ccctcgtcac gaaaccgcct ggccatcacc tggccgatgt ttccatccat cgatgcgccg
     1801 aagatcacgg cgcgcttgcc ttcgagtcgt cccatcgtcc cctccctgtc caacactggc
     1861 gagctgacgt tcattgtaca ggggttcaaa tgaccttagg ctagccgagc cactggtccg
     1921 ggtcgacaga cgatggaaga acccaagccg aagccgctgg ctcgttcgtc cacaccatcc
     1981 gcgcaggagc tggtgcaccg agacggacgc ccggtgccgc ctgttttcga gctggaaagc
     2041 ccccgattcc tgggcgacga agacattcct ttcgaacgtt acacgagcca ggaattcttc
     2101 aatcgggaga tggagcggat gtggacccga acgtggcaat gggcctgccg cgaagagcac
     2161 gtcgccgacc ccggcgacta ctacgtgtac gagatcggtc cgcactcggt tctgatcgtt
     2221 cgggatgcgc ttggggaggt cagagcctac gtcaacgctt gccttcaccg cggaaccaag
     2281 ttcaggtcag gcggctccga tggcgctttc agcgagatac gctgcccttt tcacggctgg
     2341 acctgggagc tggacggcag cctgaagcgg gtgccctacg cctgggacgc gccccacgtc
     2401 agcgagcgcg agttctcgct gccccgggtg gccgttgggc tgtggggcgg gttcgtcttc
     2461 gtcaacctcg atcccgaccc gccgcctttt gacgagtgtc tcgcgccgat tcccgagcat
     2521 ctcgagaact tcgatctgtc gaagcgtttc gtggagctgc acatcgagaa ggagctcaac
     2581 tgcaactgga aggccgccat agaggccttc atcgagaact accacaccca ggagacccac
     2641 ccccagctgc tctacggcag cgacgacgag ggcactcagt acgacgtctt caccgaccac
     2701 gtgtcccgct tcttctgcgc cttcggagtc ccaagcccca gtatcgaccc gcccccgacg
     2761 gagcaggagc tggtggacat gatgctggtc ggtgaccggt cggtgctcgg cgacgccgtg
     2821 acggtaaagc ccggagaaac cgctcgaatc gtcatggccc gggtgctccg tcagaccctg
     2881 ggcgagaaat atgcagccga cctcggccgt tacaccgaca ccgagatgat cgacgtctct
     2941 cagtacggcc tgtttccgaa catggtgctg tttccgcagc tgtcgctgcc aatggtctac
     3001 cgattccgcc ccatcggcga gaacccggac cggacgctgt tcgagctgct gatcctccgg
     3061 ccggcacccg accagggtcg cccaccggct ccggcgcgcg cccatcgcct tgaggagcgc
     3121 gacagcttca ccacggtgcc cggcttcgac accagcttag gcgaggtgta cgaccaggac
     3181 acgggcaacc tccgcgctca gcaggaaggg ttccgggcgt cacgcaagca cggcgccacc
     3241 ctgctcaacc accaggaagt ccgggttcgc cacctgcacc agaccctcga cgactatctg
     3301 cagcagtaga agccgtctag gcgagaaacg tccggggcga gaagtagccg tgaaactgat
     3361 ggggcaggtg atggctaagg tgcagggtac agacccgctc attgacgtcg tcggcccggt
     3421 agacctgcag ctcgctgcgg tgtgagtagc cgtcgtaaac gacttccaga aggtagccgt
     3481 cgtcttcgcg ggtcgcgccc tctgcagccg cgaacatggg ctccgagccg tagcagccgg
     3541 ccggcagggt gacgacgtct gcatcgccat cgaaggtcac tctctgaaaa ccgttgaaga
     3601 agctgttggc gccgttttcc accgagcagg ccgtccaggt caccgagtgc ggctgcccgg
     3661 ctttcgacgg gttgaagacg gggaactcgg cctcgcattc actcacccgt gcgcagctca
     3721 tggttccatc ggccaggttc aggcggtagc ggttgtagta cccgccgccg aaccttcccg
     3781 acgggttgaa cacatctgcc agcgtcgcgt tgacctcgaa gttatcctgg aacatggcgt
     3841 ccacgaccac ctcgtcaccg ctttcgaacg cgttgccgaa gtgaatgatc gcgcccggat
     3901 ccgtctcgaa ctcgcgcacc acctccaggg tgtcgagatc aacgacgatg atgcgcatgg
     3961 ggatgtcgtc atcgaaccgc acgccgtccg acatgctgtg tcttcccagg aagaaaccgc
     4021 ccatgccgcc aaagacgatg gagttgacga agtacacggc gtggcgcccg gccagggcga
     4081 aatcgtggca gaacggaaac gtctgcagcg gaagctgcgc cgtctttgcc atgcgaccct
     4141 ggggattgat gcggtacata ttcaggcacg gtgatttcct cacggtgccc gtggtcccgg
     4201 cgccgaagtt gaagtagtcg cccgtctgcg gatgcacctt gccgtgggcg ctgaacgcct
     4261 tgcccgcgag cgcgccgtcg tagtcgaact cgcccaccgt ctctaaagag cccggctcga
     4321 gctcccacgg gcgaccgccc tcgttcagcg ccagcagccg gccaccgtgg tggatgatgc
     4381 tggtgttcgc ggggttggcc ggccacttga agatgttctt ggtccagcct ccggggatct
     4441 gcgtgccgaa tccccgatag aggatctcct gcgcttcggt ctcctcgacg tacttgggcg
     4501 ttctgacgta tcggttgcgg aagtgggctt tggaatcggt aaacgtgaac tggctgatca
     4561 tgccgtcacc gtcgaaccag tgaccaaagg gcttgccgcc gatcttctga cgccccggac
     4621 cattgcgcat gaacgtgccg gtgaggtccg caggaatcgt tccgtcgatg tcgtctatcc
     4681 agtagtcgtg ctcctcgtcc agattctcca ggccgccgtg acaggtaggc ggctcgttgg
     4741 cgcctcgcat taccgtgctc atgtcatgaa accttaatca gtgggatgcc gaaattctcg
     4801 ccgcgcagaa tccggatgag cgccgacgga acgctctcga tgccctcgag aacgtcctcg
     4861 cgatacttca gacgcccgtc gcggacgagg gtggtcagcc gggcgagaga ctcatcgaaa
     4921 cggtgcgcga agttggagac gaagtagccg gacatctccg cctgcttgta aaccaggttg
     4981 aaatagttct tggggccata ccagtcgagg tcgttgttgt actgcgacgt gccgccacag
     5041 aggaccacgc gggcgcccat ggcgatctgc ccgagcaccg catcgagcgt ctcgcccccg
     5101 acgttgtcga agtagacgtc tatgccgttc gggcaggttt cggccagacg cgcggcaacg
     5161 tcttccgtgc ggtagttgat gcaggcctcg gcaccgagct cgtcgaccac ccactcgcac
     5221 ttctcgtcgc tgccggcaat gccgatcacc cgacagccct gcaggcggcc gagctggcag
     5281 acgagcgcgc ccacgctgcc cgccgcggtt gagaccacta aggtgtcgcc cacccggggt
     5341 ttgcagaact cgatcatccc gaagtaggcc gtgtgcccgc ttcccccgag cacgtgcatg
     5401 agggtcgtag gtgacagccg ttcaggcacc gcgaccttcc gcaccccacg ccggcgcgcg
     5461 ccgtcagaga ccacgtactc ctgccagccg aggtctccca tgaccaggtc tcccgcggca
     5521 aaggccgggt tgcgagactc caccacctcg gccaggccgc tgccgtacat ggtccggcca
     5581 atggggatgg gcttcccggc tataccgccg gaaaccaacg ccatgcgcat gggcgggttc
     5641 acggtgaggt atcgggtctt gagcagcagg gtgccgggct ccaacccaga aaccggttcc
     5701 tccacgagtt cgaaattgcc cggttccacc tcgacttccg gccgagacgc caccgcccac
     5761 tttctgttga tcaggccgct gatgttctac cccctctact cgtccgtgcc catcgtaccg
     5821 gtagccgtct aatcacacgt cagccgacga gcacctcggt gcggatggag ttcgctatga
     5881 agcaatgctc gtgcgcctga tcgtgcaggt cgtccagttc cgcttccgta ggcgcgttgc
     5941 cggcgtattc gatcgccgga ttgagcgtga tctcgctgat ccagtacgca ccctgggcgt
     6001 tcttggtcat ggtgcccacc gcctcgtcgc gatagccgat caccgaaaat cccgcacggc
     6061 cggcgatgtg caggaaggta agcatgtggc agctggccac cgccgcgacg aaggcctctt
     6121 ccggatctac gcaggcagga ttcgaaaacg gctccggcac caccgccggc gtgggcgatg
     6181 cctgaaccac cgcaccgcca tcaaacgtcc agcggtgctc gcgggaatag cggccgctga
     6241 cgaagtcccc ttcccccatg gtccactcga cgcaggctgt gtggtttgac atgacgtttc
     6301 tccaaccaga ggcaacaatc ctacgccgag acagacccca cgaccatggc ggctgtcaat
     6361 cgccagcgat ctccacgagc acctcgttgc gtcgcagaaa ccacggcgtg aaggggtcgt
     6421 tatagcgggc gaggaccact tgccctacgg gctcgaggcc gccggcgcgc accgaactca
     6481 gcagcgccgt ctccgcagcc ttcacattct cctcgagcca tcggcccgag aagcggcgcg
     6541 ccgccaccaa ccgcggcgga atccgcttga tctcaacgcg cggatccaca ggcgcgggca
     6601 gtgacgcctc ggagtacttg cgttccatca cgaactggta cgtccacagc ttctcttgcg
     6661 atgggtcaac cgatgcctcg tcttctgctc cggcaggctc gctgaaaacg ggcgcggtca
     6721 tggccattgt gctgcctgaa gacgtgtcgg catcgtagct caccaccggc gtggtcatgg
     6781 ccatctcccg acgctccacg ttgttgccga agatgtaccc cgctagcacg gggaacgccc
     6841 gggtacctgc ctggtcgaac tccccggaca cccgcgtctg cgcgacgagg tagccttcgt
     6901 agcgacgaac ttcgtagcca tcacctttcg agatcaccgc gtattcgggc tgttcaagag
     6961 tcgtgcccca g
//
LOCUS       DBNHQR010000305         3190 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1232446, whole genome shotgun sequence.
ACCESSION   DBNHQR010000305 DBNHQR010000000
VERSION     DBNHQR010000305.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1232446"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="AC16VR_06845"
     CDS             <1..294
                     /locus_tag="AC16VR_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016421 - CoA carboxylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HIR6010912.1"
                     /translation="TELDVRVATPTAASLMTHMLEKQAPDLSKFLLSPMPGLLVRLRV
                     AEGEEAKAGEELAVVEAMKMENSLRAEQDVKIAKVLAAEGDSLEVDQPILEFE"
     gene            408..599
                     /locus_tag="AC16VR_06850"
     CDS             408..599
                     /locus_tag="AC16VR_06850"
                     /inference="COORDINATES: protein motif:HMM:NF018058.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HIR6010913.1"
                     /translation="MEKFRLGLSAAIAILLIILILQNMVTVEVRLLWWTVAMPRAILL
                     LLTAMAGFAVGILVRIRRR"
     gene            complement(613..>3190)
                     /locus_tag="AC16VR_06855"
     CDS             complement(613..>3190)
                     /locus_tag="AC16VR_06855"
                     /inference="COORDINATES: protein motif:HMM:NF016260.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HIR6010914.1"
                     /translation="SGTLSGPLAALNVRHRLEGPVTLEAQGTVDLSSLPDPRFELEVA
                     MPEFSSEGFLLSDVLATVSGGRERVEGVVEGLVSTPWLTEPVPARLSGSADLPPGEVR
                     IAVDGFQAEVLGRPVEGRLVARQLAAGWRLEDVELDLSGSRVSGSAELDESGARASVH
                     ISAPDLASLDLGVAGDASGSVDFAAEAGSLSVSVSVSGSELSSGTARLLDYSVSGESR
                     GREMNFVVRGDRAAQAGIEARMPSLQVDGPIDAPAWQLEWQGGAANGIATREGEGLNL
                     RVEALRFVALESSWRLSAPVKASADAAGLRVSAFCIEGPGSSGCVDELEMSGGKLVTA
                     GRLVELPADLVSQWLPLPLVEGAVLEGQWRVVRDDRAWSGSAGLAARRLAYAVAGPGS
                     VPIELPDVTVAAVFDDQVLRYTAEASDTDFELALDGQLSPIAANGSLAGTLRLQADDV
                     GPLRAFYQHVRELTGRLEGSVEFGGTLEEPRGAGSISLRDGSLSLERPDLELRELQAL
                     LTLTEAGSVEVTGSASQADGRIELRGSGQDLFAAARAFDVELTGSDLAVEHPDWTVRV
                     SPDLRFTYTAGRGHLAGLIELPQAEIKVETLPAGAPRVSDDVVVVGRDEQADTRRTRV
                     TVDVTLVLGDQVALNAAGIAAQLEGRLNARVDAEGRQSLHGALQIEGGAVLTQGQTLT
                     IESGNVVYNGPVDNPYIDLRATRTIDTESPSVTVGVHIRGSADNLTSTIFSDPAMSET
                     RALSYLILGRDIGDPSDTDTSQLAAAAINLGLRSSSSIVADLQRIAGLDELSAVAETQ
                     KSFAIVAGKRIGPSLYVRYTYNTLSTIGALIVRYDLTRRWRVEALSSEYSAMDLFYRI
                     GR"
BASE COUNT          588 a         1112 c         1010 g          480 t
ORIGIN      
        1 acggagctgg acgtccgcgt ggccacgcca accgcggcaa gcctgatgac gcacatgctc
       61 gaaaaacagg cgcctgatct gagcaagttt ctgctctctc ccatgccggg tctcctggtc
      121 cgactcaggg tcgccgaggg cgaagaggca aaggccgggg aagagctcgc ggtggtcgaa
      181 gccatgaaga tggaaaacag cctgcgcgca gagcaggacg tcaagatcgc caaggtgctc
      241 gcggcggagg gggacagcct ggaagtcgac caaccgattc tcgagttcga gtaggccggc
      301 tcacgcccat ccgggattac cgaaaaccgt ccacagcaga tcgcagcact gcccaatgtt
      361 gaagctcgcc cttagaatgc gccctgactc caccttccgg taagaccatg gaaaaattca
      421 ggttgggctt gagtgccgcc atagcaattc tgctgatcat cctgattctg cagaacatgg
      481 tgaccgttga ggtccgtctg ctctggtgga cggtggccat gccaagagcc attctgctgt
      541 tgctgaccgc catggccggg ttcgcggtcg gcattctcgt tcggatacgc agaagatgag
      601 aagctcacgg ctctagcgcc cgatcctgta gaaaagatcc atggcggagt actcgctgga
      661 aagcgcctcc acgcgccagc gcctggtgag atcgtaacgg acgatgagcg cgccgatggt
      721 cgaaagggtg ttgtacgtat agcggacgta caggctgggc ccgatccgtt ttccggcaac
      781 gatggcaaag ctcttctggg tctcggcaac cgccgacagc tcatcaaggc ccgcgatgcg
      841 ctgcaggtcg gcgacgatgg acgacgagga ccggagcccg aggttgatcg ctgcggccgc
      901 aagctggctg gtatccgtat ccgacgggtc gccgatgtcg cggcccagga tcagatagga
      961 aagcgcccgc gtctcggaca tcgcggggtc cgagaatatc gttgaagtca gattgtcggc
     1021 gctgccgcgg atgtgcacgc caaccgtgac agacggactc tcggtgtcga tggtccgggt
     1081 agcgcgcagg tcgatgtagg gattgtccac cgggccgtta tagacgacgt tgccggactc
     1141 gatggtcagc gtctgaccct gggtaagcac ggccccgccc tcgatctgca gggcaccgtg
     1201 caacgattgc cgcccctcgg catccacccg agcgttgaga cggccttcca gctgagcggc
     1261 gatccccgct gcgttgagcg ctacctggtc tcccaggacc agcgtcacgt ccaccgtcac
     1321 gcgggtgcgg cgcgtgtcag cctgctcgtc ccgaccaacg accaccacgt cgtcggaaac
     1381 acgcggcgcg ccggcgggaa gggtctctac cttgatctcg gcctggggca gctcgatgag
     1441 ccccgcaaga tgtccgcggc cggccgtgta ggtgaaccgc aggtcgggcg acacccgcac
     1501 cgtccagtcg ggatgctcca cggcgaggtc tgaccccgtc agctcgacgt cgaacgcccg
     1561 ggcggcagcg aagaggtcct gccccgagcc tcgcagctcg attcggccat cggcctggga
     1621 ggcgctgccc gtcacctcga cggatccggc ctcggtcagc gtcaacaacg cttgcagttc
     1681 cctgagctcg aggtccggac gctccaggct gagacttcca tcgcgcaaag agatcgagcc
     1741 tgcaccccga ggttcttcca gcgttccgcc gaactctacc gagccttcaa gtcgcccggt
     1801 gagctcgcgg acatgctggt agaaggcccg caaaggcccg acgtcgtcgg cctgcaggcg
     1861 cagggtgccc gccaacgagc cgtttgccgc gatgggcgac agctgaccat ccaaggccag
     1921 ctcgaagtcc gtatccgagg cctcggcggt gtaacgcagg acctgatcat cgaagaccgc
     1981 cgcgaccgtc acgtcgggca gctcgatggg cacgctgccc gggccggcaa ccgcatacgc
     2041 cagccgccgg gctgccaggc cggcactgcc cgaccaggcc cgatcatccc ggacgacccg
     2101 ccactggccc tccaggaccg cgccttccac cagcggcagg ggcagccatt gagacacgag
     2161 atccgcaggc agctccacca ggcggccggc cgtgacgagt ttgccgccgc tcatctcgag
     2221 ctcgtcgacg cagccactcg agcccggccc ttcgatgcag aaggcagaca cccgcagccc
     2281 ggccgcgtcc gctgaggcct ttaccggcgc gctcagccgc cagctcgact ccagagcgac
     2341 gaaccgcaac gcctcaacgc gcagattcag accctctccc tcacgggtgg cgatcccgtt
     2401 ggctgcgccg ccctgccact cgagctgcca ggctggcgcg tcgatcggac cgtcgacctg
     2461 caggctcggc atgcgggcct cgatccccgc ctgcgccgcg cgatcgccac gaacgacgaa
     2521 gttcatctcg cggccgcgac tctccccaga caccgagtag tccagcagcc tggccgtacc
     2581 gctggagagc tcgcttcccg agacactcac ggacaccgag agcgaccccg cctccgcggc
     2641 aaaatcgacg gagccggacg cgtcaccggc gaccccgaga tccagagagg ccagatccgg
     2701 tgcgctgatg tgcaccgagg cacgcgcgcc ggactcgtcg agctccgcag acccggacac
     2761 acggctgccg ctcaagtcca gctccacgtc ctcgagccgc catcccgctg ccagctgccg
     2821 cgccaccagc cgaccctcca cgggcctgcc tagcacctcg gcctggaaac cgtccacggc
     2881 aatgcgaacc tcgccgggcg gaaggtccgc actgccggac aggcgagcag gcacgggctc
     2941 cgtgagccac ggtgtggaga cgagcccctc gaccacgcct tccacccgtt cgcggccgcc
     3001 gctcacggtg gccaggacgt ccgacagcag aaagccttca ctcgaaaatt cgggcatcgc
     3061 gacctcgagc tcgaaacggg gatccgggag gctcgaaagg tctaccgtac cctgcgcttc
     3121 cagcgtgacc gggccttcca ggcgatgtcg aacattcagg gcggcaagcg ggccgctcag
     3181 cgtgccagag
//
LOCUS       DBNHQR010000306         1559 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1105342, whole genome shotgun sequence.
ACCESSION   DBNHQR010000306 DBNHQR010000000
VERSION     DBNHQR010000306.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1105342"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /locus_tag="AC16VR_06860"
     CDS             <1..1173
                     /locus_tag="AC16VR_06860"
                     /inference="COORDINATES: protein motif:HMM:NF012321.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation protein NorD"
                     /protein_id="HIR6010915.1"
                     /translation="TAEGEEAVAADVEGLSDGDIRPADVDLRALSNERDVLARRLDME
                     RATIRDAVGRPRPDARSYRYDEWDYLNRSYRTRWCRLFEERLAPDTQQDLDALRHVIR
                     RHRRAVQSQLEHIKPQGYQRVTRVADGDELDFNAVIEARGDIRAGLTPDERVYSRRER
                     VHRDVCAAFLVDLSASTDDPVDPPEAVAAGAVEELANLRDPYEFPVAHDEPAEEPRRI
                     IDVQRESMLVMSAALEQLADSYGIYGFSGYGRECVEFFVAKEPDEPFTRGTLSAIAGM
                     KPKRSTRMGPAIRHATSKLLRSGHALKVLIILSDGFPQDSDYGPERGDHEYGVQDTAK
                     ALREAEEKGVETFCVTVDRSGHDYLKRMCPNARYMVIEETRDLPGALSKVYEALTG"
     gene            complement(1193..>1559)
                     /locus_tag="AC16VR_06865"
     CDS             complement(1193..>1559)
                     /locus_tag="AC16VR_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HIR6010916.1"
                     /translation="ANGDVLIASALGTTALAFVGLSAFALVTRKDFSFLSGFLVVGFF
                     VLMGAVLLAWLFDLSGFHLAISCGFVLFACAAILWQTSAIIHGGETNYILATITLYVQ
                     IYNLFISLLHIFGAFGSDD"
BASE COUNT          304 a          505 c          530 g          220 t
ORIGIN      
        1 acggcagaag gcgaggaggc cgtcgctgcg gacgtggagg gcctctccga cggcgacatc
       61 cgccctgcgg acgttgacct gcgggcgctg agcaacgagc gcgacgtcct tgcccgacgg
      121 ctcgacatgg agcgggcaac gattcgcgac gccgtgggca ggcctcgacc ggatgctcgc
      181 agctaccgct acgacgagtg ggactatctg aaccgctcgt accgcacgcg atggtgccgg
      241 ctgttcgagg agcgcctcgc cccggacaca cagcaggacc tcgacgcgct gcggcatgtg
      301 atccgccgcc accgccgggc agtgcagagc cagctggagc acatcaagcc ccagggatat
      361 cagcgggtca cccgggtggc ggacggtgac gagctggact tcaacgcggt gatcgaggcc
      421 cgcggcgaca tccgcgcagg gcttaccccg gacgagcgcg tctacagccg ccgggaacgc
      481 gtgcaccggg acgtctgcgc tgcctttctc gtcgatctgt ccgcgtccac ggacgatccg
      541 gtggatccgc ccgaagccgt cgcggccggc gcggtcgagg agctcgccaa cctgcgcgat
      601 ccctacgaat ttccggtcgc ccacgacgag ccggccgagg agccccgtcg catcatcgac
      661 gtccagaggg aatccatgct cgtcatgtcg gccgccctgg agcagctcgc agacagctac
      721 ggcatctacg gcttttccgg atatggccgc gagtgcgtcg agttcttcgt cgccaaggag
      781 ccggacgagc cgttcacgcg tggcaccctg tccgccattg ccgggatgaa gcccaagcgc
      841 agcacccgca tgggcccggc catacgccac gcaacgtcca agctgctgcg ctcaggtcac
      901 gccctgaagg tgctgatcat cctgagcgac ggctttcccc aggactccga ctacgggccg
      961 gagcggggcg atcatgaata cggcgtgcag gacaccgcca aagcgctccg ggaggccgag
     1021 gaaaagggcg tggagacgtt ctgcgtcacg gtggaccgct cggggcacga ctacctcaag
     1081 cgcatgtgcc ctaacgcgcg ctacatggtc atcgaggaaa cccgggatct gccgggagcg
     1141 ctcagcaagg tctacgaagc gctcaccggc tgatcaggat ctgtcgcggc gttcagtcgt
     1201 cgctgccgaa cgctccgaaa atgtgcagca acgagatgaa caggttgtag atctgcacgt
     1261 agagggtgat ggtggcgagt atgtagttgg tctcgccgcc gtggatgatg gcgctcgtct
     1321 gccagagaat ggccgcgcag gcaaacagca cgaagccgca ggatatggcc aggtggaagc
     1381 cggagaggtc aaaaagccag gccagcagca ccgcgcccat cagaacgaag aagccgacca
     1441 cgaggaagcc gctcaaaaag ctgaagtcct tgcgggtgac cagcgcgaac gcggagagcc
     1501 cgacgaaggc gagcgccgtg gtgcccagag ccgaggcgat caggacgtca ccgttggcg
//
LOCUS       DBNHQR010000307         4194 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_852240, whole genome shotgun sequence.
ACCESSION   DBNHQR010000307 DBNHQR010000000
VERSION     DBNHQR010000307.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4194)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_852240"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="AC16VR_06870"
     CDS             complement(<1..546)
                     /locus_tag="AC16VR_06870"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6010917.1"
                     /translation="MNVKPTTDAPNAVGHRGVVSALVTALSDLVGRRDQLEPMLPDER
                     LDGRTYLVTGASSGLGKAAAVRLASAGAEVIMACRSGIPEAGDDVMRASGSGQVRMLE
                     VDLFDLESASAMCDRLRDDKQHLDGVVFNAGLIPLSARRGPQGFEEMFAVHFLSSRLN
                     VERLLADGVVAPSVDPATRPRL"
     gene            complement(543..1751)
                     /locus_tag="AC16VR_06875"
     CDS             complement(543..1751)
                     /locus_tag="AC16VR_06875"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family protein"
                     /protein_id="HIR6010918.1"
                     /translation="MRAFNRMGPALGATGLLPALDAEHMMAEARRRAGLSDFGEDWFR
                     APLDALLGSINGEARLSALGRTIIRKRIVDALTTRLRARALLDAYPEIESFDPGRVLV
                     IAGLQRTGTTLLHRLLGADPRIRAVLSWEALNPVPLSGPAERERRIRQALRAERGLKY
                     IAPEFFAIHPVEHDMPEEDVLLLDVSFMSQSAEATMHVPTYARWLEEHDHAPAYRYLR
                     QLLSVLLWQRNAPTLLLKSPHHLEHLDTVLDVFPNATIIQTHRDPQQTVPSFASMVCH
                     GASIFSDRVDVPRLTDHWLRKIRRVVERSLVARERHGTARFVDVSYYDLIGDPMAELA
                     RVYEAAGLAFDPQAQQGARETLRRQARDRFGRHRYRREDFGLTRELIEGELGFYRRRF
                     DIPFEEEAAS"
     gene            complement(1780..2961)
                     /locus_tag="AC16VR_06880"
     CDS             complement(1780..2961)
                     /locus_tag="AC16VR_06880"
                     /inference="COORDINATES: protein motif:HMM:NF018452.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HIR6010919.1"
                     /translation="MRKLAVGLLGLFRRGALLWHKMRGRTLDDVAAERIVSGRSWEEF
                     CDTLKAAGAALTFPGAPRDPFNQAEGYRYLTRLTRAGLEAFVEYADAAAPVIHRVAHE
                     TVKLGSDNPDNYYQTARINGAWDYRITGRRNTIHYLSFGTQIGHYGQGGGMPPTGFID
                     DKDLEIDPDGSFTILLSPRRQPGNWLPMTEETGTLIVRQTYLDRAAEEPADLSIERVD
                     GGGTPGQVTPALMDAGLKDASTLVAGASLLFAKWARDFQSHTNRLPMFDQEVSNRAGG
                     DPNITYYHSHWTLAPDEALVIEALPPACEHWNFQLNNYWMESLDYRYHTIHTNKHLAT
                     YRPDGSVRIVVAHQDPGVPNWISTVGHASGTMSFRWIRAEATPEPTTRVVRLADVASL
                     A"
     gene            complement(2969..3433)
                     /locus_tag="AC16VR_06885"
     CDS             complement(2969..3433)
                     /locus_tag="AC16VR_06885"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6010920.1"
                     /translation="MFPRAVVASVDIQAPAREVWAVLVDFARYPEWNPFTPEVEAELA
                     VGAPVRLRVNMPGRRELERVEQINRVEPERALCWGMHMGHRALLCANRWQVLEPVRAA
                     TRYTTADRFSGLLVPLVMALYGGPMQLGFQQMADALKVRVEGTLPAGGAAGR"
     gene            complement(3530..3904)
                     /locus_tag="AC16VR_06890"
     CDS             complement(3530..3904)
                     /locus_tag="AC16VR_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6010921.1"
                     /translation="MLGYATIGVNDMDRAVAFYDALLAELGAKQLMGMDRIKMYGTSP
                     DAGMLAICIPYDEEPQSPGNGNMVAISAGSREAVDRLYAKAMELGAADEGEPGERNPV
                     FYGAYVRDLDGNKLCFYEMKLG"
     gene            4053..>4194
                     /locus_tag="AC16VR_06895"
     CDS             4053..>4194
                     /locus_tag="AC16VR_06895"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HIR6010922.1"
                     /translation="MAQASRPLRADSAATRESILEASVKLFGERGYRNTSLRAITDAA
                     DVN"
BASE COUNT          703 a         1416 c         1378 g          697 t
ORIGIN      
        1 gagccgtggc cgggtcgccg ggtcgacact cggcgcaacc acaccgtccg ccagcagccg
       61 ctcgacgttg agccggctgg acagaaagtg cacggcgaac atctcctcga agccctgggg
      121 cccgcgccgc gcagaaagcg gtatgagccc ggcgttgaaa acgacaccgt cgaggtgctg
      181 cttgtcatcc cgaagacggt cgcacatggc cgaggcgctc tcgaggtcga aaagatccac
      241 ctccagcatc ctgacctgcc cgctgcccga cgcgcgcatg acgtcgtcgc cggcttcggg
      301 aatcccgctc cggcaggcca tgatcacttc cgcgcccgcg ctcgccaggc gcacggccgc
      361 cgctttgccc aggccgctgc tggcgccggt gaccagatat gtccgaccgt ccagccgctc
      421 gtcaggcagc atcggctcga gctgatctct tcgtccgacc agatcgctca gcgccgtgac
      481 aagcgcgctc acgacgccgc ggtgcccgac ggcgttgggc gcgtccgtgg tcggcttgac
      541 gttcatgatg cggcctcttc ctcgaagggg atgtcgaaac gcctgcggta gaaccccagc
      601 tcgccctcga tcagttcccg ggtcagaccg aagtcctcac ggcgatagcg atgccggccg
      661 aagcggtcgc gggcctgccg gcgcaacgtc tcgcgggcac cctgctgcgc ctgagggtca
      721 aaggccaggc cagcagcctc atagactcga gcgagttcag ccatcggatc gccgatgagg
      781 tcgtagtagg agacgtccac gaagcgcgcc gtcccgtgcc gctcccgggc caccagggag
      841 cgctccacca cccgacggat cttgcggagc cagtgatcgg tgagcctggg gacgtccacc
      901 cgatcgctga atatcgaagc cccgtgacag accatgctgg caaacgacgg caccgtctgc
      961 tgcgggtcgc ggtgggtttg aatgatcgtc gcgttcggaa aaacgtcgag tacggtatcc
     1021 agatgctcca ggtgatgagg tgacttgagc aacagggtcg gcgcgttgcg ctgccacagc
     1081 agcacgctca gaagctgcct gagataccga taggcgggcg cgtggtcgtg ctcctccagc
     1141 cagcgagcgt aggtgggaac gtgcatggtc gcctcggcgg actggctcat gaacgagaca
     1201 tcgagcagca gcacgtcttc ctcgggcatg tcgtgctcga cggggtgaat ggcaaagaac
     1261 tccggcgcga tgtacttcag tccccgttcg gcgcgcaggg cctggcggat cctgcgctca
     1321 cgctctgccg ggccgctcag gggcaccggg ttgagcgctt cccacgagag caccgcccgg
     1381 atgcgcggat cggcgccgag gaggcgatgc agaagggtgg ttcccgtgcg ctgcagcccg
     1441 gcgatgacca gcaccctgcc cgggtcgaag gactcaattt ccggatacgc gtccaggagc
     1501 gcccgggcgc gcagacgcgt ggtgagagcg tcgacgatgc gcttgcggat gatggttctg
     1561 cccagagcgc taagccgggc ctcgccgttg atcgagccca acagggcgtc cagcggcgcg
     1621 cggaaccagt cttcgccaaa gtccgaaagg cccgcgcgcc gacgcgcttc cgccatcatg
     1681 tgctcggcat ccaatgctgg caggagcccc gtcgcgccaa gcgcggggcc catccggttg
     1741 aaggcgcgca cgggccacgg gagccgcgga gggcggaagt caggcaaggc tcgccacgtc
     1801 ggctagcctg acgacccggg tggtcggctc cggcgtcgcc tccgcccgaa tccagcggaa
     1861 gctcatggtt cccgaagcgt gacccacggt gctgatccag ttcggaaccc ccggatcctg
     1921 gtgagcgacc acgatccgca ccgagccgtc cgggcggtag gtggccaggt gcttgttcgt
     1981 gtgaatcgtg tggtagcggt agtcgaggct ttccatccag tagttgttca gctggaagtt
     2041 ccagtgctcg caggccggcg gcagggcctc gatgaccagc gcctcgtcgg gcgcgagcgt
     2101 ccagtgactg tgatagtaag ttatgttggg gtccccgccc gcccggttgg agacctcctg
     2161 atcgaacatg ggcaaccggt tggtgtggga ctggaaatcc ctcgcccact tggcgaacag
     2221 gagcgatgcg cctgccacca gcgtgctcgc atccttgagg cccgcgtcca tcagtgccgg
     2281 ggtcacctga cccggcgtgc cgccgccgtc cacccgctcg atagacagat cggcgggctc
     2341 ctcggccgca cggtcgaggt aggtctgacg gacgatgagg gttcccgtct cttcggtcat
     2401 gggtaaccag ttaccgggct gccggcgagg gctgagcaag atggtgaacg agccgtccgg
     2461 atcgatctcc aggtctttgt cgtcgatgaa gcccgtgggc ggcatgccgc ccccctgacc
     2521 gtagtggccg atctgggtgc cgaagctcag gtagtggatc gtgtttcgcc tgccggtgat
     2581 gcggtaatcc caggcgccgt tgatgcgcgc cgtctggtag tagttgtcgg gattgtcgga
     2641 gccgagcttg accgtctcgt gggcgacgcg atggatgacc ggcgccgcgg cgtcggcata
     2701 ctccacgaaa gcttcgagcc ccgcccgggt cagccgggtg aggtagcggt agccttcggc
     2761 ctggttgaag ggatcgcgcg gagcgcccgg aaaggtcagc gccgcgccag cggctttgag
     2821 ggtgtcgcag aactcctccc aggaacggcc gctgacgatg cgctcggcag ccacgtcgtc
     2881 cagggtccgc ccgcgcatct tgtgccagag cagcgcaccc ctgcgaaaca ggccgagcaa
     2941 ccccaccgcg agttttctca tgatctcctc atcggccggc agctccgccg gcgggcagcg
     3001 tgccttctac acgcaccttc agcgcgtcgg ccatctgctg gaagccaagc tgcatcgggc
     3061 ccccgtagag cgccatgacc agcggcacca ggagaccgct gaaccggtcc gcggtggtgt
     3121 atcgcgtggc cgccctcacc ggctcgagaa cctgccagcg gttggcgcac agcagggccc
     3181 gatgacccat gtgcatgccc cagcagagcg cgcgctccgg ttccacccga ttgatctgct
     3241 ccacccgctc tagctcgcgc cgacccggca tgttcacccg caggcgaacc ggcgcgccca
     3301 cagccagctc cgcctctacc tcaggcgtga acgggttcca ttccggatag cgggcaaaat
     3361 ccaccagcac tgcccagacc tcccgggccg gcgcctggat gtccacgctg gcaaccacgg
     3421 cgcgaggaaa cacgcgcccc tgccggtacc gccgatgaag tcgctcgctt tcaccgtccg
     3481 tcgcgggcgc cgcctgagcg gatcgcatga ccggatcgcc gcctggcggc taccccagct
     3541 tcatctcgta gaagcagagc ttgttgccgt ccagatccct gacgtaggcg ccatagaaga
     3601 cgggattgcg ctcgccgggt tctccctcgt cggcagcacc gagctccatc gccttggcgt
     3661 agagccgatc cacggcctcc cgggatccgg cggatatggc caccatgttg ccgttgccgg
     3721 ggctttgagg ttcttcatcg taagggatgc agatggccag catgccggca tccggactgg
     3781 ttccgtacat cttgatgcgg tccattccca tgagctgctt tgcacccagc tccgcgagca
     3841 gcgcatcgta gaaggcgacc gcccgatcca tgtcattcac cccaatggtt gcgtagccca
     3901 gcatgagagg ccctcctttt ggctcgttga gagatagcga gcggcattgt cgcgccgccg
     3961 cgccccgcgt gccagccgag ttcgcaatca gcgcgtgaca gtcccgagca ccgcgtgcta
     4021 atttaaacga ccgtttgaac taggaatgcc gcgtggctca ggcatccaga ccgttgcgcg
     4081 cagactcggc agccacccgg gaaagtattc tcgaagcctc ggtgaagctt ttcggcgagc
     4141 gcggctaccg caacacgtcg ctgagggcca ttaccgacgc cgccgacgtg aacc
//
LOCUS       DBNHQR010000308         3656 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_218396, whole genome shotgun sequence.
ACCESSION   DBNHQR010000308 DBNHQR010000000
VERSION     DBNHQR010000308.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_218396"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1299
                     /gene="thrS"
                     /locus_tag="AC16VR_06900"
     CDS             <1..1299
                     /gene="thrS"
                     /locus_tag="AC16VR_06900"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017516489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIR6010923.1"
                     /translation="DSRNEMLQRIYGTAWATDKDLNAYLVRLEEAEKRDHRRLGREMD
                     LFHLQEEGPGAVFWHPKGWTMFQRMIDYMRRKQIEAGYQEVNTPEILDRSLWERSGHL
                     EKFGENMYLTQTPDDRVYAIKPMNCPGHVQIFRQGIKSYRDLPLYLAEFGKVHRYEPS
                     GALHGLMRVRAFVQDDAHIFCTAEQITEECVKASNLIIEIYRDFGFEDVTVKYADRPE
                     RRVGSDEVWDNAESALITALEASGLQWTLNPGEGAFYGPKLEFVLRDAIGRDWQCGTV
                     QVDLNLPERLGATYVAADGSRQYPVMIHRAMFGSLERFCGILIEHYAGHLPLWLAPLQ
                     AVVATITDEADEYARSVMAAARAAGLRVEADLRNEKISYKVREHSVTKVPVMLVVGKR
                     EAEEGTVAIRRLGGKAQEVLPLDAALAALGAEALSPLDNH"
     gene            1318..1875
                     /gene="folE"
                     /locus_tag="AC16VR_06905"
     CDS             1318..1875
                     /gene="folE"
                     /locus_tag="AC16VR_06905"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HIR6010924.1"
                     /translation="MRDRDRLLEAFTTIIEEVDDIDRPGVVDTPRRAADSMLFLTRGY
                     QQTLDDVVNDALFPSDNREMVVVQDIELYSLCEHHLLPFIGRCHVGYIPDGKVLGLSK
                     VARIVDMFARRLQIQEELTFQIANALTEVTGAADVAVIIEARHLCMMMRGVEKQNSLM
                     KTSAMLGAFRENPSTRTEFLNLLKD"
     gene            complement(1900..2073)
                     /locus_tag="AC16VR_06910"
                     /pseudo
     CDS             complement(1900..2073)
                     /locus_tag="AC16VR_06910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rdx family protein"
     gene            2202..3473
                     /locus_tag="AC16VR_06915"
     CDS             2202..3473
                     /locus_tag="AC16VR_06915"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6010925.1"
                     /translation="MNFEIPEDIAELLDRLDDFIEQVIKPLEQQDDNIRFFDHRREDA
                     RTDWDRGGLPNEEWEQLLFKVKRLADEAGFYRYPFPKEYGGMNGNNLGMAIIREHLAV
                     KGLGLHNDLQNEHSIVGNQVGLLLMLEYGTDEQKAEWVDDLANGVRGFAFGITEPNHG
                     SDATHMETHAERDGDEWVINGEKTWNTGIHKAKYDMVMARTSGKPGDGDGITAFLTPM
                     EADGVKIEEMLWTFNMPTDHGRVSFTNVRVPHVSIFGGEGRGLQVVQHFFNENRIRQA
                     ASSLGAAQFCINESVRYAQERKPFGKALATNQGIQFPLVELQTQCEMLRALIHKTAWE
                     MDTYGAFSVSDKVSMCNYWSNRLCCESADRAMQVHGGLGYSRHKPFEHIYRHHRRYRI
                     TEGAEEIQMRRVAGYMFGFMSQRAPKGVRES"
     gene            3476..>3656
                     /locus_tag="AC16VR_06920"
     CDS             3476..>3656
                     /locus_tag="AC16VR_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010926.1"
                     /translation="MDHAEFEARLGEVLTRRIDRVRSLRSAERLSGGASQETYRLTVD
                     TADGEELLAMRRAPGG"
BASE COUNT          721 a         1119 c         1179 g          637 t
ORIGIN      
        1 gactcccgca acgagatgct tcagcgtatt tacgggacgg catgggcgac tgacaaggat
       61 cttaacgcat atctggtgcg gctggaggaa gccgagaagc gcgatcaccg gcggctcggc
      121 cgggaaatgg atctgttcca cctccaggaa gagggtcccg gagccgtgtt ctggcacccc
      181 aagggctgga ccatgttcca gcgcatgatc gactacatgc gccgcaagca gatcgaggcg
      241 ggatatcagg aggtgaacac ccccgagatt ctcgaccgct cgctctggga acgttccggc
      301 catctggaga agtttggcga gaacatgtac ctcacgcaga cgcccgacga tcgcgtctac
      361 gccatcaagc cgatgaactg cccgggtcac gtgcagatct tcaggcaggg catcaagagt
      421 tatcgggatc tgcccctgta tctggccgag ttcggcaagg tgcatcgcta tgagccctca
      481 ggggccctgc acgggctgat gcgcgtccgg gcgttcgtcc aggacgacgc gcacatcttc
      541 tgcacggcgg agcagatcac cgaggaatgc gtgaaggcat ccaacctcat catcgaaatc
      601 tatcgtgatt tcggattcga ggacgtgacg gtgaagtacg cggaccgccc cgagcggcgc
      661 gtcggctcgg acgaggtctg ggacaacgcc gaatccgcgc tgataacggc cctggaagcc
      721 tcggggctcc agtggacgct gaatcccggc gagggagcct tctacgggcc gaagctggag
      781 ttcgttctgc gcgatgccat cgggcgcgac tggcagtgcg gcaccgttca ggtagacctc
      841 aacctgcccg agcggctcgg cgcgacgtac gtggccgccg atggcagccg tcaatacccg
      901 gtcatgatcc atcgagccat gttcggctcc ctggagcgct tctgcggcat cctgatcgag
      961 cactacgcgg gccacctgcc gttgtggctc gcgccgctgc aggcggtggt cgcgacgatc
     1021 acggacgagg ccgacgaata cgctcggtcc gtcatggcgg ccgcccgggc ggcggggctt
     1081 cgggtggaag ccgatctgcg caacgagaag atctcttaca aggtccggga gcacagcgtc
     1141 accaaggttc ctgtgatgct ggtggtgggc aagcgcgagg ctgaagaagg caccgtggcg
     1201 atccgtcggc tcggtggcaa ggcccaggaa gtcctgcccc tcgatgccgc tctggcggcg
     1261 ttgggcgctg aagccctatc acctctcgac aatcattaat ccgggggagc aaacgacatg
     1321 cgtgaccgcg acaggctcct agaggctttc acgaccatca tcgaagaagt ggacgacatc
     1381 gatcgccccg gcgtggtcga tacgccgcgc cgcgcggcgg actcgatgct gtttctcacc
     1441 cgtggctacc agcagacgct cgacgacgtc gtgaacgacg cgctgttccc ctccgacaac
     1501 cgggaaatgg tcgtcgttca ggatatcgag ctctacagcc tctgcgagca tcacctgctg
     1561 cccttcatcg gtcgctgcca cgtcggctac attccggacg gaaaggtact tggcctctcg
     1621 aaggttgccc gcatcgtcga catgtttgcg cgcaggctgc agattcagga agagctgacg
     1681 tttcagatcg cgaacgcgct gacggaggtc acgggagctg ccgacgtcgc cgtgatcatc
     1741 gaggccaggc acctctgcat gatgatgcgc ggggtggaga agcagaactc tctgatgaaa
     1801 acttccgcca tgctcggggc cttccgcgag aatcccagca cgcgcacgga gtttctgaac
     1861 ctcctcaagg actgagggcg ccgccgccag cctcgccggt cagccgccga gggaagcgac
     1921 caggtccgcc tcgtccggaa actggccggt ttcgtgcttg gagtaaacgg tgcggccgtc
     1981 cacgaccaca tcgaatatcc cgctgccccc gccgacgagc tcggcctcga caccgaaggc
     2041 ttctcgtatc ctggcagcca gactgaccgc cttgggttgg tagtttcact gcacgcagta
     2101 ggtgatgctt acgttcaagg cgcaggccct ttgcaatccg atggtcaggt cattttaatt
     2161 gggctgcggg gaatgccaaa cgaacattta aggggaccca tatgaacttc gagatcccgg
     2221 aagacatcgc cgagctgctg gaccgcctgg acgacttcat cgagcaggtg atcaagccgc
     2281 tggagcagca ggacgacaac atccgcttct tcgaccaccg ccgcgaagac gcccgaacgg
     2341 actgggatcg gggcgggctg ccaaacgagg aatgggagca gctgcttttc aaagtgaagc
     2401 gtcttgcaga cgaggccggc ttctaccgct acccctttcc gaaggagtac ggtggtatga
     2461 acgggaacaa cctgggcatg gccatcatcc gggagcatct cgccgtcaaa ggcctgggtc
     2521 tgcacaacga tctgcagaac gagcattcca tcgtcggcaa ccaggtcggc ctgctgctca
     2581 tgctcgaata cggcaccgat gagcagaagg ccgagtgggt cgacgatctc gcgaacggcg
     2641 tccgcggctt cgccttcggt atcaccgagc cgaaccacgg cagcgatgcc acccatatgg
     2701 agacccacgc cgagcgtgac ggcgacgagt gggtgatcaa cggggagaag acctggaaca
     2761 cgggcattca caaggccaag tacgacatgg tgatggcgcg gacgtcgggc aagcccgggg
     2821 acggggacgg catcaccgcg ttcctgacgc ccatggaagc cgatggcgtc aagatcgagg
     2881 agatgctgtg gacgttcaac atgcccacgg atcacggccg ggtatcgttc accaacgtgc
     2941 gcgtgcccca cgtttcgatt ttcgggggcg aagggcgcgg gctgcaggtg gttcagcact
     3001 tcttcaacga gaaccgcatc cgtcaggccg cctccagcct cggggccgcc cagttctgca
     3061 tcaacgagtc tgtcaggtat gcgcaggaac gaaagccgtt cggcaaggcg cttgcgacca
     3121 accagggtat ccagtttccc ctcgtcgagc tgcagaccca gtgcgagatg ctccgggccc
     3181 tcatccacaa aaccgcctgg gaaatggata cgtacggtgc gttctccgtt tcggacaagg
     3241 tgtccatgtg taactactgg tcgaaccgtc tgtgctgcga atcggccgat cgcgccatgc
     3301 aggttcacgg cggtctgggg tactcgcggc acaagccttt cgagcacatc tatcggcacc
     3361 atcggcgcta ccggatcacc gagggcgcgg aggaaatcca gatgcgtcgg gtagccggat
     3421 acatgttcgg attcatgtcg cagcgcgcgc ccaagggtgt cagggaaagc tgaaggtgga
     3481 ccacgccgag ttcgaagcgc ggctgggtga agtgctgact cggcggatcg accgcgtccg
     3541 ctcgctgcgc tctgccgagc gcctgtccgg gggagccagc caggagacgt atcggcttac
     3601 cgtcgatacg gctgacggcg aggagctcct ggccatgcgc cgggctcccg ggggcg
//
LOCUS       DBNHQR010000309         2160 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1297189, whole genome shotgun sequence.
ACCESSION   DBNHQR010000309 DBNHQR010000000
VERSION     DBNHQR010000309.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1297189"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /locus_tag="AC16VR_06925"
     CDS             <1..1028
                     /locus_tag="AC16VR_06925"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HIR6010927.1"
                     /translation="ENYQRVTVDQKRMINCRADLNQLVPFKYDWAWQKYLDGCANHWM
                     PQEINMTADIALWKSESGLTQDERLVVKRSLGFFSTADSLVANNLVLALYRLITNPEC
                     RQYLLRQAFEEAIHTHAYQYCIESLGMDEGETFNMYHEVPAVARKAGWALKYTRDISD
                     PTFQTGTPQADKALLKNLIAYYCVLEGIFFYCGFTQILSMGRLNKMTGTSEQFQYILR
                     DESMHVNFGIDVINQIKLENPGLWDEELQTLARQMILEGTQLEIEYAKDTMPRGILGM
                     NANMMAEYLQFIANRRLAQIGLAEQFAGAKNPFPWMSEIMDLKKEKNFFETRVTEYQT
                     GGALSWD"
     gene            complement(1053..1400)
                     /locus_tag="AC16VR_06930"
     CDS             complement(1053..1400)
                     /locus_tag="AC16VR_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010928.1"
                     /translation="MAGMRGLVFGMLMFLSASSAAREMQVEGDVVRVIPLTASSVVVE
                     RSGDCAPVKPIGQADLVALLAWDLRADCHTQRRKVEVQDGYRVYYRWDERVYSVVMTE
                     RPGPTIPLRVNVR"
     gene            complement(1403..>2160)
                     /locus_tag="AC16VR_06935"
     CDS             complement(1403..>2160)
                     /locus_tag="AC16VR_06935"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR6010929.1"
                     /translation="RGGAAPASRAGAAAHRDGCAMSRAAPLLAALLLCGTGHAVTQYP
                     YLSCFEAASRLHDVPVDLLLAVAATESNWDPDARSAANAHGIMQIQWPGTARHLGVGR
                     LSELYNPCLNIERGAKYLSELLARSGGAEDRALAAYNYGPSRIASSSTLPAGALRYVS
                     TVQGHRERIVRKTGARQASATRAAEHAVRFDTAHRARRYARSLDERVEGADFVTRAEP
                     EGGFAVVMRIAAGGLSLTDLNVLERLGWPVRGD"
BASE COUNT          431 a          704 c          661 g          364 t
ORIGIN      
        1 aggagaacta tcagcgggtc accgtggatc agaaacggat gatcaactgc cgcgcggatc
       61 tcaaccagct tgttccgttc aagtacgact gggcctggca gaagtatctg gacggctgcg
      121 cgaaccactg gatgccgcag gaaatcaaca tgaccgcgga catcgctctg tggaagtcgg
      181 agtccgggct cacccaggac gagcggctcg tggtcaagcg cagcctcgga ttcttctcga
      241 ccgccgactc cctggtcgcc aacaacctcg tccttgcgct ctaccggttg atcaccaatc
      301 cggagtgtcg acagtatctc ctgcgacagg cattcgaaga ggccatccac acccacgcgt
      361 accagtactg catcgaatcg ctggggatgg acgaaggcga gaccttcaac atgtaccacg
      421 aggttcctgc tgtggcccgc aaggccggct gggcgttgaa gtacacccgg gacatctcgg
      481 acccgacgtt ccagacgggc actccccagg cggacaaggc gctgctgaag aatctgattg
      541 cctattactg cgtgctggag gggatcttct tctactgcgg gttcactcag attctgtcca
      601 tgggacgcct caacaagatg acgggcacct ccgagcagtt tcagtacata ctgcgggacg
      661 aatccatgca cgtgaacttc ggcatcgatg tgatcaatca gatcaagctc gagaacccgg
      721 ggttgtggga cgaggaattg cagactctcg cccggcagat gatcctcgag ggtacccagc
      781 tggaaatcga gtacgccaag gacaccatgc caagaggaat actcggcatg aacgcgaaca
      841 tgatggccga gtacctccag ttcatcgcaa accggcgtct ggcgcagatc gggcttgccg
      901 agcagtttgc gggagcgaag aacccgttcc cgtggatgtc cgagatcatg gatctcaaga
      961 aggagaaaaa cttcttcgag acccgggtca ccgagtacca gactggcggg gcgctcagct
     1021 gggattgagg gcagctgagg gcgccgccgc cctcagcgaa cgttgacgcg aagcgggatc
     1081 gtgggtcccg gtctttcggt catcacgacg ctgtaaaccc gctcgtccca tcggtagtac
     1141 acgcggtaac cgtcctggac ctccaccttg cgtcgctgcg tgtggcaatc ggcccgcaga
     1201 tcccaggcca gcagagcgac cagatcggct tgcccgatcg gcttgacggg cgcgcagtcg
     1261 ccgctgcgtt ctaccaccac cgacgaggcg gtgagcggaa tcactcgaac gacgtcgccc
     1321 tctacctgca tttcccgggc agccgacgag gcgctcagga acatcagcat cccaaagacc
     1381 aatccacgca tccccgccat cgtcaatctc cccgtaccgg ccaaccgagc cgttccagca
     1441 cgttcagatc ggtaaggctc agcccaccgg ccgcgatgcg catcaccacg gcaaacccgc
     1501 cttccggctc ggcgcgcgtc acgaaatcgg caccttcaac gcgctcgtcc agcgaacggg
     1561 catagcggcg ggcacgatgc gccgtatcga agcgcaccgc gtgctcagcg gctcgcgtcg
     1621 cagacgcctg gcgcgctccg gtcttccgga cgatgcgctc gcgatggccc tgcaccgtcg
     1681 agacgtacct cagggcgccg gccggcagcg tgctgctgga ggctatcctg ctcgggccgt
     1741 agttgtaggc cgcaagcgcc cggtcctcgg cgccgccgct tcgcgcgagc agctcactca
     1801 gatatttcgc gccgcgctcg atgttcagac aggggttgta cagctcggag agtcggccga
     1861 caccgagatg ccgggcggtg cccggccact ggatctgcat gatcccgtgg gcgttggcag
     1921 cggatctggc atccggatcc cagttgctct ccgtagctgc cacggccagc agcaggtcca
     1981 caggcacgtc gtgcagccgg gaagccgcct cgaaacagga aagatacgga tattgcgtga
     2041 ccgcatgacc ggtaccgcag agcagcagag cggccagcag gggcgcggcg cggctcatgg
     2101 cgcatccgtc ccgatgagcg gccgcacctg ctcgagaagc cggcgccgct cctcctcgtc
//
LOCUS       DBNHQR010000310         4069 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1676292, whole genome shotgun sequence.
ACCESSION   DBNHQR010000310 DBNHQR010000000
VERSION     DBNHQR010000310.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1676292"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="AC16VR_06940"
     CDS             <1..727
                     /locus_tag="AC16VR_06940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HIR6010930.1"
                     /translation="VAGILLFAAGASAPAAAEGPEFSGNIAYLTDYRFRGISQGDRSQ
                     ALQGGFDIELDNGLYAGTWASNVGFTECCGSAGLELNYYAGFAGAFSEEVDYDVGFLW
                     YNYPEDDADPDLDYYELYGSVSAFGGTFGAAFSPDYFAETGDFWYLYGDYSFGVAENL
                     SIDLHVGFNLFEDEESFAAFIGPEDGDAGDNYIDYSIGVSTSALGLDFGLSFIGTDLK
                     KSECFGGTKLCDDTVVLSVSKSL"
     gene            856..1194
                     /gene="glnK"
                     /locus_tag="AC16VR_06945"
     CDS             856..1194
                     /gene="glnK"
                     /locus_tag="AC16VR_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HIR6010931.1"
                     /translation="MKLVTAIIKPFKLDDVREALSDIGVQGMTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEIAIDDGLLEQCLETITKSANTGKVGDGKIFVSSLEDVVRI
                     RTGETGAEAV"
     gene            1236..2489
                     /locus_tag="AC16VR_06950"
     CDS             1236..2489
                     /locus_tag="AC16VR_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIR6010932.1"
                     /translation="MEDLLTQTNYALDTFYFLVMGALVMFMAPGFAMLEAGLVRSKNT
                     AEILLKNVALFGIACIMYLICGYYIMYVGAVEGGILPDFGLWIGAENTVDETLASGGD
                     VYYSARSDYFFQVVFVATAMSIVSGAVAERMKLWAFLAFAVVMTGLIYPLQGMWTWGG
                     GWISTAGYSDFAGSGIVHMCGAAVALAGVLLLGPRKGKYNKDGSVNALPGANLPLATL
                     GMFILWFGWFGFNGGSELKTSNIDEANAVAQVFVNTNIAACGGLVAALLTAKMLFGKA
                     DLTMALNGALAGLVAITADPLSPIAQWAMIIGAVGGVLVVFSIVTLDKLKIDDPVGAI
                     SVHGVVGIWGILAVVFNNGDATIGGQLIGIAGIFAWTFVAALVVWGILKATMGIRVSE
                     EEEYEGVDLSECGLEAYPEFAKVSS"
     gene            2594..3955
                     /gene="thrC"
                     /locus_tag="AC16VR_06955"
     CDS             2594..3955
                     /gene="thrC"
                     /locus_tag="AC16VR_06955"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR6010933.1"
                     /translation="MRYVSTRGETASLGFTDAVLTGLAPDGGLLVPEHVPQVSARLDA
                     WADLSFTDLACEIIPLFVDDMSRQDLNVLIRQAYACFDHDEVVPFVRLGDLEVLELFH
                     GPTLAFKDIALQLLGRLFEHILSARGRHLNILGATSGDTGSAAIAGVRGLPDIDIFVM
                     YPHGRISPLQELQMTTVLDDNVHCLSVDGSFDDCQTLMKTVFADLAFKERYALGAVNS
                     VNWARVLAQIVYYGYASLKRGMEFPVSFCVPTGNFGNVFAGYLAKRMGFPIERLILAT
                     NENDILAVFFETGTYQRGDVRFTVTPAMDIQVASNFERFLYYHMGEDSRPLRAFMTEF
                     AETGRAVAAIPGDDDFVATAVSVAETVEAIRATYSEFGYVADPHTAVGIAAAGRFPAE
                     GAKICLATAHPAKFPETVNGAVGAEVAVHPALEALSGLESRTTRIPATVEAVKEAIQA
                     SAR"
BASE COUNT          724 a         1266 c         1295 g          784 t
ORIGIN      
        1 tgtggcggga atcctcctct tcgcagcggg tgccagcgcg ccagccgccg ccgaaggacc
       61 ggagttctcc ggcaacatcg catacctgac cgactaccgc ttccgcggta tctcccaggg
      121 agatcggtcc caggccctgc agggcggctt cgacatcgag ctggacaacg gcctctacgc
      181 gggaacctgg gcgtccaacg tcggcttcac ggaatgctgc ggctccgcgg gactcgagct
      241 gaactactat gctggttttg ccggtgcgtt cagcgaggaa gtcgattacg acgtcgggtt
      301 cctctggtac aactatcccg aggacgacgc ggacccggac ctcgactact acgagcttta
      361 cggcagcgtc agtgccttcg gcggcacgtt tggggctgcc ttctctcccg actacttcgc
      421 cgagaccggc gacttctggt acctctacgg cgactacagc ttcggcgtcg ccgagaactt
      481 gagcatcgat ctgcacgtgg gcttcaacct cttcgaggac gaggaatcct tcgcagcgtt
      541 catcgggcct gaagacggcg atgccggaga caactacatc gactacagca tcggcgtgtc
      601 cacgtcggcg ctgggtctcg acttcggcct ctctttcatc ggcacggacc tcaagaagag
      661 cgagtgcttc ggcggcacca agctgtgcga cgacaccgtc gtgctctcgg tatctaaaag
      721 cctgtaattg agagagaatc cggggtgcac agatcttcca caaagatctt cccagagcag
      781 tccgcgtcag cgggctgcgc tcggggagag cgtgcacctt agaaaccgaa aacgggttca
      841 acgggagaac aaaacatgaa actggtaaca gccatcatca agcccttcaa actcgatgat
      901 gtacgggagg cgctctcgga tatcggagtg caggggatga cggtcactga agtgaaaggg
      961 ttcggtcgcc agaaagggca caccgaactc tatcgggggg cggagtacgt ggtcgacttc
     1021 ctgcccaagg tgaagatcga gatcgccatc gacgacgggc tcttagagca atgcctggag
     1081 accatcacca aatcggccaa caccggcaag gtgggcgacg gcaagatatt cgtttcctcg
     1141 ctcgaagacg tcgtgcggat ccgcacggga gagacggggg ccgaggcggt ctgacgccat
     1201 gcgctagtcg cctttaaaaa aattcggaga agattgtgga agatctgttg actcaaacta
     1261 attacgccct ggacaccttt tattttctgg tgatgggtgc gctcgtcatg ttcatggcgc
     1321 ccggtttcgc gatgctcgag gccggactgg tacggtccaa gaacacggcg gaaatccttc
     1381 tgaagaacgt cgccctgttc ggcatcgcgt gcatcatgta cctgatctgc gggtactaca
     1441 tcatgtacgt cggcgccgtc gaggggggga tcctgcccga cttcggcctg tggatcggtg
     1501 cggagaacac ggtggatgaa accctggcct cgggaggcga cgtctactac tcggcgcgct
     1561 ccgactactt cttccaggtg gttttcgtgg ctaccgcaat gtccatcgtc tcgggggcag
     1621 tcgccgagcg gatgaagcta tgggccttcc tggcctttgc ggtggtcatg acgggcctga
     1681 tttacccgct gcagggcatg tggacctggg gcggcggctg gatctccacg gccggctact
     1741 cggacttcgc cgggtccggc atcgtgcaca tgtgcggcgc ggcggtagcg ctggccgggg
     1801 tcttgctgct cgggccaaga aagggcaagt acaacaagga cgggtcggtg aacgcgctgc
     1861 ccggcgccaa cctgcccctg gcgaccctcg ggatgttcat cctctggttc ggctggttcg
     1921 gattcaacgg cggctcggag ctcaagacgt cgaacatcga cgaggcgaac gcggtggccc
     1981 aggtgttcgt caacaccaac atagctgcct gcggcggctt ggttgccgcg ctgctgaccg
     2041 ccaagatgct cttcgggaag gccgatctga ccatggctct gaacggggcg ctggccggtc
     2101 tcgtggccat cacggcggat ccgctctcgc ccatcgctca gtgggcgatg atcatcggcg
     2161 ccgtcggcgg ggttctagtg gtgttttcca tcgtgacgct cgacaagctg aagatagacg
     2221 acccggtagg tgccatctcg gtccacggcg tggtcggtat ctggggcatc ctcgccgtgg
     2281 tcttcaacaa cggcgacgcc accattggtg gccagctgat cggcatcgcc gggatattcg
     2341 cctggacctt cgtcgcagcg ctcgtcgtct gggggatcct gaaggccacc atgggcattc
     2401 gggtctcgga agaggaagag tacgaaggcg tcgacctttc ggagtgcggt ctggaggcct
     2461 atccggagtt cgccaaggtc tcctcctgag cttcgcccca gcggggcgga cggcaacgaa
     2521 cgccggccac attcgtggcc ggcgttttcg tctgtaacgg gtacaatgcc caatttttca
     2581 ggcacccgtc cccatgcgat acgtcagcac ccgcggcgag actgcaagcc tcggattcac
     2641 cgatgccgtt ctgaccggtc tggccccgga tggcggcctg ctggtgccgg agcacgtgcc
     2701 ccaggtgagc gcccggctgg atgcgtgggc cgatctgtcc ttcaccgacc tcgcctgcga
     2761 gatcatcccg cttttcgtcg acgacatgtc gcgccaagat ctgaacgtcc tgatccgtca
     2821 ggcatacgcc tgcttcgacc acgacgaggt ggttccgttc gtccggctgg gagatctgga
     2881 ggtgctggag ctctttcacg ggcctaccct ggccttcaag gacatcgctc tgcagcttct
     2941 cggccgtctc ttcgagcaca tcctctcggc ccgcggccgg catctgaaca ttctcggtgc
     3001 gaccagcggc gataccggca gcgcggccat cgccggcgtt cgcgggctgc ccgacataga
     3061 catcttcgtc atgtatcccc acgggcgtat cagcccgctg caggagctgc agatgaccac
     3121 ggtgctggac gacaacgtcc attgcctttc ggtggacggc agcttcgacg actgccagac
     3181 gctcatgaaa accgtcttcg cggatctggc attcaaggaa cgttacgcgc tgggcgccgt
     3241 caactccgtg aactgggcga gagtgctggc gcagatcgtc tactacggct acgcgagcct
     3301 gaagcggggc atggagtttc ccgtctcctt ctgcgtgccc accggaaact tcggcaacgt
     3361 cttcgcgggc tacctggcca agcgcatggg atttcccatc gaacggctga ttctggcaac
     3421 caacgagaac gacatcctcg ccgtgttctt cgaaacgggc acctaccagc gaggcgacgt
     3481 gcgatttacc gtgacgcccg ccatggacat tcaggtggcc agcaatttcg agcggttcct
     3541 ttactaccac atgggcgagg acagccgacc gctgagggcg ttcatgaccg agttcgccga
     3601 aaccggtcgt gcggttgccg cgatacccgg cgacgacgac ttcgtggcca ccgcagtctc
     3661 tgtggccgag accgtcgaag cgatccgcgc cacctactcg gagttcggct acgtagccga
     3721 tccgcacacg gccgtcggca ttgctgctgc cggccgcttc ccggccgaag gcgcgaagat
     3781 ctgccttgcc accgcgcacc cggccaagtt cccggaaacg gtcaatgggg cggtgggtgc
     3841 cgaggtcgcc gtgcacccgg ccctggaggc cctgtcggga ctcgagtcgc gtacgacccg
     3901 gattccggcc accgtcgagg cggtcaagga ggcgattcag gccagcgccc gctgagggcg
     3961 attcggatca gcggccgcgc ccggcgatgc gggatacggc cgtgggaatg tcgtgcaccc
     4021 ttgcgacctc gtccgagggc ttcggggtcc cgggcggcag cgcctcgcc
//
LOCUS       DBNHQR010000311         7375 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_852961, whole genome shotgun sequence.
ACCESSION   DBNHQR010000311 DBNHQR010000000
VERSION     DBNHQR010000311.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7375)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7375)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7375
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_852961"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="AC16VR_06960"
     CDS             <1..559
                     /locus_tag="AC16VR_06960"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF027666.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase family protein"
                     /protein_id="HIR6010934.1"
                     /translation="IQLPDVNKPYRGWKLTHFEGGTHVPFMARWPARIAPGSSFDAPV
                     HHVDLFHTIVAAAGAAIPRDRKLDGVDLGPYVTGQAAGVPHETLFWREGHHQSVQHRG
                     WKLIRSARPDKRWLFDLANDPTEQVDLAGARPDKVEELVALLDAHNAEQSDPMWPSVL
                     DSPMLVDKHGGQPYEEGDEYIYWPN"
     gene            589..1587
                     /locus_tag="AC16VR_06965"
     CDS             589..1587
                     /locus_tag="AC16VR_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HIR6010935.1"
                     /translation="MDAVRTPDDRFENLADYPFAPDYVNVDDGQGGALRMHYVDEGEG
                     EVVLCLHGQPSWSYLYRKMIPLLVDAGYRAVAPDLIGFGKSDKPTSRANYTYAAHVHW
                     LNSFVEALDLKQVTVIGQDWGSLIGLRVVAENPDRFARIVLANGALPDAKGIPEAMAP
                     QLKAMLADTPALPIEEMFAKLSGPMEDRPPFMYWVRYCDENPDFHPADVMKGSLAHCS
                     EEEYRAWAAPFPSEEFIQGARQFPSLVPIIPDNPAIPANRAAWSFYEQCDKPFLTAFS
                     DSDPVTKGQEQRFIDSVPGAQGQKHTIIRGGGHFLQDDVGEELAAVVIEFMQDNPL"
     gene            1597..2364
                     /locus_tag="AC16VR_06970"
     CDS             1597..2364
                     /locus_tag="AC16VR_06970"
                     /inference="COORDINATES: protein motif:HMM:NF024117.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HIR6010936.1"
                     /translation="MSARYKPPRRRNLYEPGSAKPFKISRSKIDRFVECPRCFYIDRR
                     LGTDRPPGFPFNINSAVDTLLKTEFDVHRSEGSQHPLQAAYGIDAIPAAHANIDVWRE
                     NFKGVQHLHEPTNLIITGAIDDLWIDGDGSYIVVDYKATAKKEPVTSMEAAWMDGYKR
                     QMEIYQWLLRRNGLDVSDTGYFVYCTGRPDAEAFDAKIDFDVHLIPYQGNDDWVEPTI
                     VALHACLNQPEIPQASWRCDYCAFVGAVNDVTGRSET"
     gene            2486..3175
                     /locus_tag="AC16VR_06975"
     CDS             2486..3175
                     /locus_tag="AC16VR_06975"
                     /inference="COORDINATES: protein motif:HMM:NF041238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylhydroperoxidase-related (seleno)protein"
                     /protein_id="HIR6010937.1"
                     /translation="MYPDDLPLSEALRNTHQKTLAKIVSPGPHWSGSERRAMAESARA
                     AEHCGLCKTRKTALSPLGVEGHHDGATTLAPTLLDAIHRIRTDPGRLTKRWFEAVAAE
                     IGEGPYVEMLSVVNSLVIVDTLHRCLGQSPPALPDPVAGGPTGEPAGDVVEAGAWVPI
                     ARAPRDMADTGLPVVPNIVRAMGLVPKAVELFFGTFRPHYALKDVKLSLSQAQAEYVA
                     SRVSAMNECFY"
     gene            3191..3550
                     /locus_tag="AC16VR_06980"
     CDS             3191..3550
                     /locus_tag="AC16VR_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010938.1"
                     /translation="MLLRASGQKENRDYDLTAVTHGERRSGVEQEDWLKRLTEEAIRG
                     EWTALARTLDAASHAIGRETAVDCLVVASAFNGITRVADATGIPLDENTEAATKQMRA
                     AVGLDAFAYAAKSARFD"
     gene            complement(3577..4809)
                     /locus_tag="AC16VR_06985"
     CDS             complement(3577..4809)
                     /locus_tag="AC16VR_06985"
                     /inference="COORDINATES: protein motif:HMM:NF019640.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6010939.1"
                     /translation="MVRPEVLEHRDTISKTPILERISAMKESLAAAGDEAQELRHLPA
                     WASKEMADQGLYRFALPLELGGENLCAREQIEVVEAVSAIDGSVGWCTQISSEINALI
                     IRRMEPEFAERIFDDWDALVCSGHGPANGPNPGRLARREGDGWRLNYQGSFASGCHNA
                     NWNYLMGAMTVDEATGKPAQASFMVPLGEFEIIDTWHTAGMRGSGSHDVRMADCYVPP
                     ENLLPFRSLAPSETWQNPTYRNPTHAIYNKAAVALGVCRGAIDSFIELAMEKTPWGLS
                     STLKDQAQVQYRIGEAQATLLAARSFVMETQDKLEEHLGPLPEDGGRLEPEWEYFWPA
                     LLACAHAAQSCRKVVDMINNTAGTTGSRMDSPLERQLRDAHQAANHALISYRHYEQLG
                     RTFVGYEPPPGYVELSRA"
     gene            complement(4903..5670)
                     /locus_tag="AC16VR_06990"
     CDS             complement(4903..5670)
                     /locus_tag="AC16VR_06990"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6010940.1"
                     /translation="MSSMRGKVALVTGASGGIGLATAIGFAERGAAVMLAARRSDELE
                     RAVREITASGGEAAAIQTDVASSEQVQEMVDRTVGTFGRLDYAVNNAGIEGVPAEFVD
                     FDEDEWDRVMNINLRGNFLCLKYQAKAMLACGHGGAIVNVGSVNSFLGFAGGAAYATS
                     KHGQVALTSSASAELAPKGIRVNIVCPGVIDTPMHRRLRGTFGDEAYDEILSRVHSRR
                     AGRPEEIASTIVFLCSDEASYLTGATITPDGGYLTTM"
     gene            complement(5700..>7375)
                     /locus_tag="AC16VR_06995"
     CDS             complement(5700..>7375)
                     /locus_tag="AC16VR_06995"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR6010941.1"
                     /translation="PFAKLSIQRCHSGSTSYLRRGRRVSAEFQFGDWTIETSLNRITR
                     ADCEQHLEPKTMAVLVFLLNRHGEVVSVDEMLDRLWTGRIVGPNALHRHIALLRRALG
                     DDPSTPSYIETITKRGYRTIASMIRLPPHGAAPSDDSQHDRQEADSWGTRIAVSPFLD
                     RTTGKSHHHLVDGMAEEVVAGLASFSDIGVIPPSALPRGDASGWDPLDTCAAVGADFF
                     LEGSVSDSASRVRVSLRLTDVANREVIWSRSFDYVLSASSYFDIQDDIKARVSAGIAD
                     SFGVVSSKAYERARTKPTSAIAADDAVMLAKGHEGNPKLHREAREGLEKAVEQHPDFA
                     NAWAWLGAMYRDEFVFWANERPNPLERAESAAQRALKLDASNQEALLVLADVRFYQGD
                     LAAFRQASERAMSLNPSSPDLLSVVASHLIHAGYYEEGKPLAQRAVDLLAQPTLHMLG
                     TLSVCHYGLGDYAAAERVIRQVWAGVDRNRISGMQAYYLTLLVAATAKLGREDEAGQY
                     LQWILKSIPNYAQIMGPSWHRQGACEDLRERLAEGLAAAGLRIGELTNR"
BASE COUNT         1300 a         2403 c         2338 g         1334 t
ORIGIN      
        1 catacagctg cccgacgtca acaagcccta tcgcggctgg aagctgaccc atttcgaagg
       61 gggcacccac gtgcccttca tggctcgatg gccggctcgc atcgcgccgg gatcgagttt
      121 cgatgcgccc gtccatcacg tggacctgtt ccacaccatc gtcgccgcgg cgggcgcggc
      181 cattccgagg gaccgaaagc tcgacggcgt ggacctcggt ccctacgtca ccggtcaggc
      241 agccggcgtg ccccacgaaa ccctgttctg gcgggaaggg caccaccagt cggttcagca
      301 tcggggctgg aagctgatcc gctcggcccg gcccgacaag cgctggctgt tcgatctggc
      361 caacgatcct acggagcagg tcgatctggc cggggcacgg ccggacaagg tcgaggagct
      421 cgtggcgctg ctggatgccc acaatgccga gcagtccgac cccatgtggc catcggtgct
      481 ggattcgccg atgctcgtcg acaagcatgg cggccagcct tacgaggaag gcgacgagta
      541 catctactgg cccaactgaa acccgcaaac agatcaggaa tacgaacgat ggacgccgtc
      601 agaaccccag acgatcgatt tgaaaatctc gccgactacc cctttgcccc tgactacgtg
      661 aacgtcgacg acggccaagg cggggcgttg cgcatgcact acgttgacga aggtgaagga
      721 gaggtcgtcc tgtgcctgca tggccagccg agctggtcct atctgtaccg caagatgatc
      781 ccgctgctcg tcgatgccgg atatcgggcc gttgcgcccg atctgatcgg cttcggcaag
      841 tccgacaagc ccacgagccg cgcgaactac acctacgcag cgcacgtgca ctggttgaat
      901 agcttcgtcg aggccctgga cctgaagcag gtcacggtca tcggccagga ctggggcagc
      961 ctcatcgggt tgcgggtcgt ggctgaaaac cccgaccgtt ttgcccgcat cgtgctggcg
     1021 aacggcgcgc tgcccgacgc caaggggatc ccggaggcca tggcgcctca gttgaaggcc
     1081 atgctggccg acacgcccgc gctgccgatc gaggagatgt ttgcgaagct ctccgggccc
     1141 atggaagacc gcccgccgtt catgtactgg gtgagatact gcgacgagaa tccggacttt
     1201 cacccggctg acgtcatgaa gggttcgctg gcccactgct cggaggagga ataccgggcc
     1261 tgggctgcgc cgttcccctc ggaagagttc atacagggcg cacggcagtt cccgtcgctc
     1321 gtgcccatca ttcccgacaa cccggcgatt cccgccaacc gtgcggcctg gtcgttctac
     1381 gagcagtgcg acaaaccgtt cctgaccgcc ttcagcgatt cggatcccgt gaccaaaggc
     1441 caggagcaac gcttcatcga ctccgtgccg ggcgcgcagg gccagaagca cacgatcatc
     1501 agaggcggtg gccacttcct gcaggacgac gttggcgagg agcttgccgc ggtggtgatc
     1561 gagttcatgc aagacaaccc gctctgaccc gttgcgatga gcgcgagata caagccgccg
     1621 cgacgccgca atctctacga gccgggaagc gcgaagccgt tcaagatctc ccggtcgaag
     1681 atcgaccgtt tcgttgagtg cccgcgttgc ttttacatcg acaggcgcct gggcaccgac
     1741 cgtcctcccg gcttcccttt caacatcaac agcgccgtgg acacgctgct gaaaaccgag
     1801 ttcgacgttc accggtcaga gggtagtcag catccgctgc aggcggccta cggtatcgac
     1861 gcgattcccg ccgcgcacgc caacatcgac gtctggcgcg agaacttcaa gggggttcag
     1921 cacctccacg agcccaccaa cctgatcatc accggcgcca tagacgatct gtggattgac
     1981 ggcgatggct cctacatcgt ggtggactac aaggcgacgg ccaagaaaga gcccgtcacc
     2041 tccatggaag ccgcctggat ggatggctac aagcgtcaga tggagatcta ccagtggctc
     2101 ctgcggcgta acgggctcga tgtgtccgat accggctact tcgtctactg caccggccgc
     2161 ccggacgcag aggcgtttga tgcaaagatc gacttcgacg ttcacctgat tccctaccag
     2221 ggcaacgacg attgggtgga gcccaccatc gtggcgctgc acgcctgcct gaaccagccg
     2281 gagataccgc aagcgagctg gcggtgcgat tactgcgctt tcgtcggtgc cgtcaacgac
     2341 gtcaccgggc ggtcagagac ttgaggcgac gaccggcccg cttcgctcag ctggtggtct
     2401 ccttgccgag ccgttacgct ctggcctgtc tgcggccggc cgttccaatt gtcggaaggt
     2461 ctcgaatccg ggggagagtg atgccatgta tccagacgac ctgccgctga gcgaagcgct
     2521 gagaaacacg caccagaaga cgctcgccaa gatcgtcagc cccggccctc actggtcggg
     2581 cagtgaacgg cgtgccatgg cggaatccgc cagagccgcc gaacactgtg ggttgtgcaa
     2641 gacgcgcaag acggccctct ctcctttggg ggtcgaaggc caccacgatg gcgccacgac
     2701 tctcgcgccg acgctcctgg atgccatcca tcgtatccgc acggatcccg ggcggctgac
     2761 caaacgctgg ttcgaggcgg tcgcggcgga gatcggcgag ggcccctacg tggagatgct
     2821 cagcgtggtc aatagcctgg tgatcgtcga cacactgcac cggtgtcttg gtcaatcacc
     2881 gcccgctttg ccggacccgg tggccggtgg gcccacgggg gagcccgccg gggacgttgt
     2941 ggaagccggc gcctgggtac cgatcgcccg cgcgccgagg gacatggcgg atacgggcct
     3001 gcccgtggtg ccgaacatcg tccgagccat ggggctggtg cccaaagcgg tagagctctt
     3061 ctttggcacg ttccggccgc attacgctct gaaggacgtc aagctttctc tctctcaggc
     3121 acaggcggag tacgtggcgt cccgagtgtc ggccatgaac gagtgcttct actgaaccac
     3181 ctcccacacg atgctgctcc gggcgagcgg ccagaaggag aacagggact acgatctcac
     3241 cgccgtcacc cacggtgaac gcagatccgg ggtcgagcag gaggactggc tgaaacggct
     3301 gaccgaagaa gctatccgag gcgagtggac ggctcttgcg cggacgctcg acgctgccag
     3361 ccacgccatc gggcgcgaga cggccgtgga ctgcctggtg gtggccagcg ccttcaacgg
     3421 catcactcgg gtggcggacg ccaccggaat cccgctggat gagaacacgg aagcggcgac
     3481 gaagcagatg cgggcggcgg tcggcctgga cgcgttcgcc tacgccgcca agagcgcccg
     3541 gttcgattag ctctcgagcc tacgccgaac gccgcctcag gcgcgggaaa gctccacgta
     3601 tcccggtggc ggctcgtagc cgacgaacgt cctgcccagc tgttcgtagt gccggtacga
     3661 gatgagcgcg tggttggccg cctgatgcgc gtcccgtagc tgccgctcga ggggactgtc
     3721 catccggctg cccgtggtgc ccgcggtgtt gttgatcatg tccaccactt tgcggcagga
     3781 ctgcgcggcg tgggcgcacg cgagcagggc gggccagaag tactcccact ccggctccag
     3841 gcgcccgccg tcttcaggaa gcggccccag gtgttcctcc agcttgtcct gggtttccat
     3901 gacgaaagac cgtgcggcca gcaacgtcgc ctgggcctcg ccgatccggt actggacctg
     3961 agcctggtcc ttgagcgtgg agctcaagcc ccagggcgtt ttctccatgg ccagctcgat
     4021 gaacgagtcg atggcgccgc ggcagacgcc gagggcgacg gccgccttgt tgtagatggc
     4081 gtgggtcggg ttccggtagg tggggttctg ccaggtttcg ctcggtgcga gagaccggaa
     4141 cggcagcaga ttctcgggcg gcacgtagca gtcggccatg cgcacgtcgt ggctaccgga
     4201 gccgcgcatg ccggcggtgt gccacgtatc gatgatctcg aactcgccca ggggcaccat
     4261 gaaggaggct tgggccggct tgcctgtggc ctcgtccacg gtcatcgcgc ccatcaggta
     4321 gttccagttc gcgttgtggc agccgcttgc aaatgagccc tggtagttca accgccaccc
     4381 atcgccctcc cttcgggcca gccggccggg attgggcccg ttcgcggggc cgtgaccgga
     4441 gcacaccaga gcatcccagt cgtcgaagat tcgttcggca aactccggct ccatgcggcg
     4501 aatgatcagc gcgttgatct ctgacgatat ctgcgtgcac caaccgaccg acccgtcgat
     4561 ggccgagacg gcctcgacca cctcgatctg ctcgcgagcg cagagattct ctccgccgag
     4621 ttccaaagga agcgcgaatc gatagagccc ctgatccgcc atctccttgg acgcccaggc
     4681 cggcagatga cgaagttcct gggcttcatc gcccgctgcc gccagagact ccttcatcgc
     4741 cgagatgcgc tccagaatgg gcgtcttgga gatcgtgtcc ctatgctcca gaacttcagg
     4801 tcttaccatt tcgattttgc ctcacaagtc agattgtccc gaagggtgaa agaataaaca
     4861 ttttgccgcg cacatgccgt aattgctcgg gctgccggtt gcctacatcg tcgtgagata
     4921 gccaccgtcg ggggtgatgg tggccccggt caggtagctg gcctcgtccg agcacaggaa
     4981 cacgatggtg ctggcgatct cttcggggcg gccggcccgc cggctgtgca cgcggctgag
     5041 gatctcgtcg taggcctcgt cgccgaaggt cccgcgcagg cggcggtgca tgggggtgtc
     5101 gatgacgccg gggcagacga tgttcaccct gatgcccttt ggcgcgagtt ctgccgatgc
     5161 agaagaggtg agcgctacct ggccgtgctt ggaggttgcg taggcggcgc cgcccgcgaa
     5221 gcccaggaac gagttcaccg agccgacgtt gacgatggcc ccgccgtggc cacaggccag
     5281 catcgctttc gcctggtact tcagacagag gaagttgccg cgcagattga tgttcatcac
     5341 gcgatcccac tcgtcctcgt cgaaatccac gaactctgcc gggaccccct cgatgccggc
     5401 gttgttgacc gcgtagtcca ggcgcccgaa ggttcccacg gtgcgatcca ccatctcttg
     5461 cacctgctcc gacgaggcga catccgtctg gatcgccgcc gcctctccgc cggaggccgt
     5521 gatctcccgc accgcccgtt ccagctcatc ggaccgtctg gccgccagca tcaccgctgc
     5581 accgcgctcc gcgaagccga tcgccgtagc caacccgatg ccgccgcttg cccccgtcac
     5641 gagcgccacc ttgcctctca tcgaactcat ctcccattgt ccttatgcaa ccgtctagcc
     5701 taacggttgg tgagctcacc gatccggagg ccggctgcgg cgagcccttc cgccaaccgc
     5761 tcgcgcagat cctcgcacgc cccttgccga tgccagctgg ggcccatgat ctgagcatag
     5821 ttggggatgc tctttaaaat ccattgcagg tactggccgg cttcgtcctc gcgcccgagc
     5881 tttgcggtgg cggcgaccag cagggtcagg tagtaggcct gcatgccgct gatccggttc
     5941 cggtccaccc ctgcccacac ctggcgaatc accctttcgg cggcggcgta atcacccaag
     6001 ccgtagtggc acacagacag cgtgccgagc atgtgcagcg ttggctgtgc cagcagatcc
     6061 acggcgcgct gggcgagcgg tttgccttcc tcatagtagc cggcgtgaat caggtggctc
     6121 gccaccacgg aaagaagatc ggggctgctc ggattcaggc tcatggcgcg ctcggatgcc
     6181 tgtcggaacg cagccaggtc tccctggtag aaccgcacgt ccgcgagaac cagaagggct
     6241 tcctggttgg aggcgtcgag tttcagcgcg cgctgtgctg cgctctccgc acgctcgagt
     6301 gggtttggcc gctcgtttgc ccagaacacg aactcgtccc ggtacatggc gccgagccat
     6361 gcccaggcat tggcgaaatc aggatgctgc tcgaccgctt tttcaaggcc ctctcgtgcc
     6421 tcacgatgca gtttcgggtt gccctcgtgg cccttggcga gcatgacggc gtcgtccgct
     6481 gcaatggccg aagtcggctt cgtccttgcc cgctcatagg ccttcgaaga gaccacgccg
     6541 aaagagtctg cgatgccggc agagacccgg gcttttatgt cgtcctggat gtcgaaatag
     6601 ctgctcgccg agagcacgta gtcgaacgat cgggaccaga tcacttctcg gtttgccaca
     6661 tcggtgagtc gcaggctgac acgaactctc gaggccgaat cgcttacgct gccttccagg
     6721 aaaaagtccg cgccgacggc cgcgcaggtg tccaggggat cccagccgga tgcatccccc
     6781 ctcggcaggg cgctgggcgg gatgacgccg atatcgctga agctggccaa ccccgcgacg
     6841 acttcctctg ccatcccatc gaccagatgg tggtgactct ttcctgtcgt tctgtcgagg
     6901 aacggcgaaa cggcgatgcg ggtgccccag gagtcggctt cctgcctgtc gtgctgcgag
     6961 tcatctgatg gcgctgcccc gtgaggggga agcctgatca tggacgcaat ggtgcgatac
     7021 ccgcgcttgg taatggtctc gatgtacgac ggtgtgctgg gatcatctcc cagggcgcgg
     7081 cgcagcagcg cgatatggcg gtggagcgca ttgggcccga cgatccggcc agtccagagt
     7141 ctgtctagca tctcgtcgac ggacaccacc tcgccgtggc ggttcagcag aaagacgagc
     7201 actgccatgg tctttggttc caggtgctgc tcgcaatctg cgcgtgtgat ccggttgagg
     7261 ctggtctcaa tggtccagtc gccaaattga aattccgccg acacccgtct cccccgtctc
     7321 aggtagcttg tcgatccgct atggcagcgc tgaatgctga gcttagcgaa gggcg
//
LOCUS       DBNHQR010000312         1602 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1549543, whole genome shotgun sequence.
ACCESSION   DBNHQR010000312 DBNHQR010000000
VERSION     DBNHQR010000312.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1549543"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="AC16VR_07000"
     CDS             complement(<1..1026)
                     /locus_tag="AC16VR_07000"
                     /inference="COORDINATES: protein motif:HMM:NF014805.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6010942.1"
                     /translation="MFRRLLIANRGAIARRVVRACNDLGIESVAVYSEADAHAPYLSE
                     ASRAVGLPGVSAGETYLNTAALLEAVRASGADAVHPGYGFLAENADFARAVAAAGATF
                     VGPDPKWLEVMGDKVAARRLLADHGFPVFPGSGGLSSLDEALSAAAELSYPVLLKPAA
                     GGGGIGMRLVRDEAELASAFASAQRLAEQAFGQGGIYLERAIDKPRHVEFQILADGAG
                     GALHLYERECSIQRRHQKIIEEAPAVGLPREELDALAARAEAVMAEIGYDNVGTLETL
                     CTQQGEFGFLEMNARIQVEHAVTEAVTGLDLVGLQIRLAAGAALPERPGLCGHAVEAR
                     IYAEDPRT"
     gene            complement(1043..1429)
                     /locus_tag="AC16VR_07005"
     CDS             complement(1043..1429)
                     /locus_tag="AC16VR_07005"
                     /inference="COORDINATES: protein motif:HMM:NF019013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="limonene-1,2-epoxide hydrolase family protein"
                     /protein_id="HIR6010943.1"
                     /translation="MSDDKAVVLELIDGFNSIDLDRIVACFTEDAVYDNIPMQAVQGT
                     EAIRASLEGFMGAASEVQWDLLNVVGEGGVVLTERIDKFKVNGTWIELPVMGTFEVAD
                     GKIKAWRDYFDLAQFQTQFAKATGAQ"
BASE COUNT          266 a          581 c          481 g          274 t
ORIGIN      
        1 cgtgcgcgga tcttccgcgt aaattctcgc ttcgacggca tggccgcaga gcccggggcg
       61 ctcgggaagc gctgcgccgg cggccaggcg gatctgcagc cccaccagat cgagccccgt
      121 aaccgcctcg gtcaccgcat gctccacctg gatgcgtgcg ttcatctcga ggaagccgaa
      181 ctcgccctgc tgcgtgcaca gcgtctccag ggtcccgacg ttgtcgtagc cgatctctgc
      241 catcaccgct tccgcgcgcg cggcgagcgc atccagctcc tcgcgcggca ggccgacggc
      301 gggcgcttcc tcgatgatct tctggtgtcg ccgttgaatg gaacattcgc gttcgtagag
      361 gtgcaacgcc ccaccagcac catcggcgag aatctgaaac tccacgtgac gcggcttgtc
      421 gatggcccgc tcgaggtaga tgcctccctg cccgaacgcc tgctcggcca gccgttgtgc
      481 agatgcgaaa gcactagcca gctccgcctc gtcgcgaacc agccgcatgc cgatgccgcc
      541 gcctccggcg gccggtttga gcagcacggg gtatgacagc tcggcggctg cgctgagagc
      601 ctcgtcgaga ctgctgagac cgccactgcc cgggaagacg ggaaaaccgt ggtcggcgag
      661 caggcgacgg gccgccacct tgtctcccat cacctcgagc cacttaggat ccggcccgac
      721 gaacgtggct cccgcagccg ccaccgcgcg ggcaaagtca gcattctccg ccagaaagcc
      781 gtacccggga tgcacggcgt ccgcgccgct tgcgcgcacg gcttccagga gggcggccgt
      841 gttgaggtac gtttccccgg cgctcacccc tggcaggccc accgcccgag aagcctcgga
      901 caggtacggc gcatgggcgt ccgcttcgga gtaaacggca acgctctcga tccccagatc
      961 gttgcaggca cgcaccaccc gccgggcgat ggccccccgg ttggcaatca gcaatcgccg
     1021 gaacacggcg cggctcgtcc ggctactgag cgcccgtcgc cttggcgaac tgggtctgga
     1081 actgagccag atcgaagtag tcgcgccagg ctttgatctt gccgtcggcg acttcgaagg
     1141 tgcccatcac cgggagctcg atccaggtgc cgttgacctt gaatttgtcg atccgctcgg
     1201 tgagcaccac gccaccctca ccgaccacgt tgagcaggtc ccactgaacc tcgctggccg
     1261 cgcccatgaa cccctccagc gacgcccgaa ttgcctcggt gccctgcacg gcctgcatgg
     1321 gaatgttgtc gtataccgca tcttcggtga agcacgccac gatgcggtcc agatcaatgc
     1381 tgttgaaacc atcgatcagc tcgagcacta ctgccttgtc atcactcacg tcgttctccc
     1441 tctttatctc ttttaccgat ctcgccggag cggcgcaccg tcgggcgcga ggcagcacaa
     1501 ctggcggtca gccccgcgtc cagttcagat actccctcca ggcctcgacg aaaccgccca
     1561 acccgcgatg cgaggtcgct tcggttgcgg cgcccgcttc cc
//
LOCUS       DBNHQR010000313         2523 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_663552, whole genome shotgun sequence.
ACCESSION   DBNHQR010000313 DBNHQR010000000
VERSION     DBNHQR010000313.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_663552"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="AC16VR_07010"
     CDS             <1..505
                     /locus_tag="AC16VR_07010"
                     /inference="COORDINATES: protein motif:HMM:NF020672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SelB domain-containing protein"
                     /protein_id="HIR6010944.1"
                     /translation="VGAPVPERFKSELLRELAGAGKLTQRAGHYLPAEHEVALSESEA
                     RLLDRISGQLDQIQPPSVGDLAKQIGVPVAELKRQLQPLAGKRRLVRVSETRYYLPAP
                     LSSLVHVVDALSSKGPFTVREFRDAADIGRNVAIEVLEYLDGRGYTKRQGDVRRVVGD
                     PSRVMAT"
     gene            518..610
                     /locus_tag="AC16VR_07015"
     tRNA            518..610
                     /locus_tag="AC16VR_07015"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:552..554,aa:Sec,seq:tca)
     gene            637..1767
                     /locus_tag="AC16VR_07020"
     CDS             637..1767
                     /locus_tag="AC16VR_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007946297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR6010945.1"
                     /translation="MPGLLPNVDPDGLLEYSVVYTDRALNHMSLAFRQVMRDISESLK
                     SVYGAASAVVVPGSGTFGMEAVARQFATGRKCLVLRNGWFSYRWSQILEMGRMPTETI
                     VLKARQAEPGTQAPFAPAPLDEVVASIAAEKPNVVFAPHVETSCGMILPDDYLREVAN
                     AVHAVGGLFVLDCIASGAIWVDMKEIGVDVLISAPQKGWTGTPCCALVMLGTGALEAM
                     EGATSTSFACDLGKWLEIMRAYENGGHAYHATMPTDGLRILADVMREIEAFGYETARE
                     RQEVLGRRVRGLLEGHGIASVAAEGYQAPGVVVSYTTDPDIQSGRKFADAGLQIAAGV
                     PLQCDEPDDFRTFRLGLFGLDKLADVDRTVASLEDALIGVLP"
     gene            1844..>2523
                     /locus_tag="AC16VR_07025"
     CDS             1844..>2523
                     /locus_tag="AC16VR_07025"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016413 - O-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase family protein"
                     /protein_id="HIR6010946.1"
                     /translation="MSATQKFTFQGTFAMRRGSISNPTFAFETLGRLNAQRDNAVLIF
                     TGLSPSAHVASSTADPSSGWWEDMVGPDKPIDTDRYFVVCVNSLGSCFGSTGPASINP
                     ETGKHYRLDFPVLTLEDVANGGAAVLDHLDVQAVNTVLGASMGGMTALAFALLHPDRA
                     RRLLSISSAARSLPFSIAVRSLQREIIRSDPGWKNGNYAFDEGPVTGMRLARKLGMIS
                     YRSADEWA"
BASE COUNT          437 a          831 c          819 g          436 t
ORIGIN      
        1 ggtcggcgcg ccggtacccg agcggttcaa gtccgagctg ctgcgcgagc tggccggggc
       61 tggaaagctc actcagcggg ccgggcacta cctgcccgcg gaacacgagg tcgcgctgag
      121 cgaatcagaa gcacgactgc tcgatcgcat ttccggtcag ctcgatcaga tccaacctcc
      181 cagcgtcggc gatctcgcca agcagatcgg cgtgccggtg gctgagctaa agcgccagct
      241 gcagcctctc gccggcaagc ggcgcctggt gcgcgtgagc gagacgcgct actacctacc
      301 cgccccgttg tcgtccctgg tccacgtcgt cgacgccttg agcagcaaag ggccttttac
      361 cgtcagggag tttcgggacg ctgccgacat cgggcgcaac gtggccatcg aggtgctcga
      421 gtatctggac ggccgcggct acacaaagcg ccagggggac gtccggaggg tggtcggtga
      481 cccgagccga gtgatggcga catgataaac tgccttcgga agagcatcgt ccccggtggg
      541 gcgcgcggca ttcaaagccg ttgaggtgcg atgacgtgcc tggtgggttc gactcctacc
      601 tcttccgcca ttttcctgct gactgacgag gaacacatgc ccggactgct gcccaacgtc
      661 gaccccgacg gcctgctcga gtattcggtg gtgtacaccg accgggcact caatcatatg
      721 tcgcttgcgt tccggcaggt catgcgggac atctccgaat cgctcaagtc ggtctacggc
      781 gcggcgagcg ccgtggtggt gcccggcagc gggaccttcg gcatggaggc cgtcgccagg
      841 caattcgcca ccggcagaaa atgtctcgtc ctgcgtaacg gatggttcag ctatcgctgg
      901 agccagatcc tagagatggg ccgcatgccc acggaaacca tcgtgctgaa agcgcggcag
      961 gccgagccgg gcacccaggc gcctttcgcc ccggcgcccc tcgacgaggt ggttgccagc
     1021 atcgccgccg agaagcccaa cgtcgttttc gcaccccatg tggagacgtc gtgcggcatg
     1081 atcctgcccg acgactatct ccgcgaggtt gccaacgccg tacatgccgt cggcgggctg
     1141 ttcgtgctcg actgcatcgc ttccggggcg atctgggtag acatgaagga gatcggcgtc
     1201 gacgtgttga tcagcgcacc tcaaaagggc tggacgggga ctccctgctg cgctctggtg
     1261 atgctgggaa caggcgccct cgaggcgatg gagggcgcca cgagcaccag cttcgcctgc
     1321 gacctcggca agtggctgga gatcatgcgc gcatacgaaa acggggggca cgcataccac
     1381 gccacgatgc ccaccgacgg gttgcgcata ctcgcagacg tcatgcgcga aatcgaagcc
     1441 ttcggttacg aaaccgcccg cgagcgccag gaggtcttgg gccgccgggt gcgcgggctg
     1501 ctcgaaggtc acggcatcgc cagcgtggcg gccgaggggt atcaggcccc cggcgtggtc
     1561 gtgagctaca ccaccgaccc ggacattcag tcgggacgga aattcgccga cgccggtctg
     1621 cagatcgctg cgggagtgcc gcttcagtgc gacgagccgg acgactttcg gacgttccgc
     1681 ctgggtctgt ttgggctgga caagctcgcc gacgtcgacc gaaccgtcgc cagcctggaa
     1741 gacgcgttga tcggcgtgct cccctgacga tgttagtatc cgccctccgc agctccgctg
     1801 ggcggatctg ttaccgacaa ctcatcggtc tggcgacggc aacatgagcg ccacgcaaaa
     1861 gttcacattc cagggcacct ttgccatgcg gcgcgggtcc atcagcaacc cgaccttcgc
     1921 ctttgaaacg ctcggtcggc tgaacgcgca gcgcgacaac gcggtgctca tcttcacagg
     1981 cctgtcgcca tcggcccacg tggcatcgtc aacagcagac ccctcctccg gatggtggga
     2041 ggacatggtg ggaccggaca agcccatcga caccgaccgc tatttcgtcg tctgcgtgaa
     2101 ctcgcttggc agctgcttcg gcagcacggg cccggcttcc atcaaccccg aaaccggtaa
     2161 gcactaccgg ctcgactttc cggtgctcac gctggaggac gtcgccaacg gcggggccgc
     2221 cgttctcgac cacctggacg tccaggcggt gaacacggtc ttgggggcct ccatgggcgg
     2281 gatgacggcg ctggcgttcg ccctgctcca tcctgatcgg gcgcgccggc tcctctccat
     2341 ctcgtcagca gcgcgcagcc tgccgttttc catcgccgtc cgttcccttc agcgcgagat
     2401 cattcgcagc gatcctggct ggaagaacgg caactacgct ttcgacgaag ggccggtgac
     2461 gggcatgcgg ctggcccgca agcttggcat gatctcctac cgatcggccg acgagtgggc
     2521 aga
//
LOCUS       DBNHQR010000314         4104 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1550022, whole genome shotgun sequence.
ACCESSION   DBNHQR010000314 DBNHQR010000000
VERSION     DBNHQR010000314.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4104)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1550022"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /locus_tag="AC16VR_07030"
     CDS             <1..1026
                     /locus_tag="AC16VR_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010947.1"
                     /translation="ATGAGFELLGPDATMLKAGVPVIAVCAVRTGCGKSQTARFVSSE
                     LVRGGRRVAVMRHPMPYGDIAAQRVQRFASMADMDAAGCTLEEREEYEPHVAAGGVVY
                     AGADYGAVLEQAEQGVDVLVWDGGNNDFSFIRPDFHIVVVDALRPEQLDTHYPGAAVL
                     RTADLVVVNKVRSASADQLDQVSAGLDRIVPGVPRVLAASPVVLDEPERLTGARVLVV
                     EDGPTITHGGMPTGAGYRAVHELPVARIIDPRTCAAGSIAALYDRYPHIGPILPAMGY
                     GPAQLDELRETVARSGADYVVAGTPIDLARALETDIPVLRARYSYVDAGSPGLMDHVK
                     DFLDERI"
     gene            1013..1885
                     /locus_tag="AC16VR_07035"
     CDS             1013..1885
                     /locus_tag="AC16VR_07035"
                     /inference="COORDINATES: protein motif:HMM:NF012899.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid kinase family protein"
                     /protein_id="HIR6010948.1"
                     /translation="MSASDSQPLLIVSLGGNALSPPGRGGEGYADERRIVAQTARTLR
                     DLAGLGRRLLIVHGNGPQVGRLLRTDPEPGNLDIHVAQTQGELGYLIARELAPEPVVG
                     VLTRVVVSEPLGAPVKPIGPILAERPQKAASVPAGSGWRLVVPSPKPARVVEIKAVAQ
                     LLKSSHVVAGGGGGVPLDEEGDPLNCVVDKDWVASLFAVGLHAQSLVFATDVSGVHPD
                     PERQGERIDRLSVADAEQLLASDAVAPGSMAPKLASAVEFVSSSGRSAGICDLESLAA
                     FAGGDESAGTVITP"
     gene            complement(1887..2348)
                     /locus_tag="AC16VR_07040"
     CDS             complement(1887..2348)
                     /locus_tag="AC16VR_07040"
                     /inference="COORDINATES: protein motif:HMM:NF039797.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6491 family protein"
                     /protein_id="HIR6010949.1"
                     /translation="MSTRILMLSMLLLAGCASAPEAEQAQQQREEDIATILNEPLDPS
                     EYGEAKRCLAGHEYRDFAIIDDRRIVFKGPGNSLWLNELRARCPDLRRADTLAFRSHT
                     MSERICELDSFVASDWMDSLSRRSPGMRCTLGSFQPISEPQLQALRDLSKR"
     gene            complement(2414..3316)
                     /locus_tag="AC16VR_07045"
     CDS             complement(2414..3316)
                     /locus_tag="AC16VR_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004420289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR6010950.1"
                     /translation="MTTEQLLRFATYASVATATVLIAAKLTAWFATDSVSVLASLVDS
                     VMDVGASLINLFAVRYSLMPADQEHRFGHGKAEALAGLGQATFIAGSAIFLILHAVDR
                     LMDPVTIEHVAAGSAVMGFAIVMTLVLLTIQRHVIRRTGSTAIRADALHYATDLATNA
                     ATLLALLLAAYGFSGLDPLFGLAIAAYILYSASRIGRDAVSLLMDRELPQAEREEIKR
                     VVDGTPGVLGVHGLRTWQSGQRKVIQMHLELDPDLPLREAHRIAVEVERRLLEHEPEA
                     DITIHQDPAGQPSHVAHNPGDAFS"
     gene            3495..3878
                     /locus_tag="AC16VR_07050"
     CDS             3495..3878
                     /locus_tag="AC16VR_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010951.1"
                     /translation="MNALVAHLLVALMLTTPLTALAGSCEGAGEAPARAAGHHVGHGP
                     EHAGYDEHHGDHGEVSVEDGAADCDCCDECLQECAASGSTSFLAGSALEAPLRSGPKR
                     FVTDWLSGHVRAPPTSLFRPPILDC"
BASE COUNT          659 a         1273 c         1443 g          729 t
ORIGIN      
        1 gccacgggtg ccgggttcga acttctcggg ccagacgcaa cgatgctcaa ggcgggcgtt
       61 cccgtcatcg ccgtgtgtgc cgtgcgcacc ggttgcggta aatctcagac ggcccgcttc
      121 gtgtcgagtg agctcgtcag gggcggcagg cgcgtcgccg tgatgcgtca tcccatgccc
      181 tacggcgaca tcgcggccca acgggtgcag cggtttgcct ccatggcgga catggacgcg
      241 gccggctgca ccctggaaga gcgggaagag tacgagcccc acgtggctgc cggtggggtc
      301 gtgtatgcgg gggcagacta cggagccgtt ctggagcagg ccgaacaggg cgttgacgtg
      361 ctcgtctggg acgggggcaa caacgacttt tcgttcattc gccccgattt tcacatcgtc
      421 gttgtcgacg cgctgcgtcc tgagcagctg gacacccact accccggtgc cgcggtgctg
      481 cgaacggccg atctggtggt ggtgaacaag gtccgcagcg cgagcgcgga tcagctggat
      541 caggtgagcg cggggctgga ccgaatcgtg ccgggtgtgc cgcgggtgct cgcggcgtct
      601 ccggtggtcc tggacgagcc ggaaaggctg accggtgccc gggtgctcgt cgtggaggac
      661 ggtccgacca tcacccacgg tggcatgccc accggggccg gctatcgggc ggtgcatgaa
      721 cttccggtcg cccggatcat cgatccgcga acgtgcgctg cgggttcgat cgccgcgctc
      781 tatgacagat atccgcacat aggccccatt ctcccggcca tggggtacgg acccgcgcag
      841 ctagacgagc tgcgcgagac ggtcgcacga tcaggcgcgg actacgtcgt ggcgggaacg
      901 cccatcgatc tcgcccgggc gctggagacg gacatccccg tgttgcgggc ccggtattct
      961 tacgtcgatg ccggatcgcc cggattgatg gaccacgtca aagactttct cgatgagcgc
     1021 atctgattcc cagccgctgc tgatcgtctc gctgggcggc aatgccctgt cgccgcccgg
     1081 gcggggcggc gaaggatacg ccgatgagcg ccggatcgtg gcgcagaccg cccggacgct
     1141 gcgcgatctg gccggcctgg gtcgccgact gctgatcgtt catgggaacg ggcctcaggt
     1201 cgggcgcctg ctgcggaccg accccgagcc cggcaacctc gacatccacg tggcccagac
     1261 ccagggcgag ctgggatatc tgatcgccag ggagcttgcg ccggagcctg tggtaggcgt
     1321 gctgacacgg gtggtcgtct ccgagccgct cggcgcgccg gtcaagccca ttggcccgat
     1381 tctcgccgaa cgccctcaga aagcggcatc ggtgcctgcg gggtcggggt ggcggctagt
     1441 cgtgccctcg ccgaaaccgg ctcgcgtcgt cgagatcaag gccgtagcgc agctgctgaa
     1501 atcgagccac gtcgtggccg gcggcggcgg cggggtgccc ctggacgaag agggtgaccc
     1561 gctgaactgc gtggtggaca aagattgggt tgccagcctg tttgccgttg gcctgcacgc
     1621 ccagagtctc gtctttgcca ccgacgtgtc cggcgttcac ccggacccgg aacgccaggg
     1681 tgaacggatc gatcggctga gcgttgccga cgcggagcag ctgctggctt cggacgccgt
     1741 cgcgccgggg tccatggctc cgaagctcgc cagcgccgtg gagttcgtca gctcatcggg
     1801 ccggtccgcg ggaatctgtg atctggagag cctcgccgcg tttgcgggcg gagacgaatc
     1861 cgccggcacg gtgatcacac cctgagtcag cgcttggaca gatcgcgaag ggcctgcagc
     1921 tgaggctcgc tgatgggctg gaaagaaccg agcgtgcagc gcatcccggg cgagcggcgc
     1981 gacaggctgt ccatccagtc cgacgctacg aagctgtcga gctcgcagat ccgctcgctc
     2041 atggtatgag atcgaaaggc cagcgtatcg gcccgccgca agtcggggca gcgagcccga
     2101 agctcgttca gccacaggct gtttccagga cctttgaaca cgatgcggcg gtcgtcgatg
     2161 atggcgaagt cgcggtactc gtggcccgcc aggcagcgtt tcgcctcacc gtactccgac
     2221 gggtcgaggg gttcgttcag aatcgtggca atgtcttcct cccgctgctg ctgggcctgc
     2281 tcggcctcgg gcgccgacgc gcagccggcg agcagcagca tggagagcat tagaatccgg
     2341 gtgctcatgg cgacgaacgt tagcccgaaa cgcggtcgac tgacaaatcg ccccgctgac
     2401 cccattggat gagtcaactg aacgcgtcgc cagggttgtg agcaacgtgg gatggctgtc
     2461 ccgccggatc ctgatggatg gtgatgtcag cctccggctc gtgttcgagc aggcgtcgct
     2521 ccacctccac ggcgatgcgg tgagcctctc tgagcgggag gtccgggtcc agctccaggt
     2581 gcatctggat gaccttgcgc tgtccggact gccaggtgcg cagcccgtga acgcccagca
     2641 ccccaggcgt gccgtccacg actcgcttga tttcctcgcg ctcggcttgg ggcagctccc
     2701 ggtccatcag caggctgacg gcgtctcgac cgatgcggct ggcggagtaa aggatgtagg
     2761 ctgcgatggc caggccgaac agcggatcca accctgagaa accgtaggcc gccagcaaca
     2821 gcgcgagcag ggtggccgcg ttggtggcca ggtccgttgc gtagtgcagc gcatccgcgc
     2881 gtatggcagt cgaaccggtg cggcggatta cgtgccgctg aatcgtcagc agcaccagcg
     2941 tcatgacgat ggcgaatccc atcaccgccg agcctgccgc gacgtgctcg atcgtgacgg
     3001 gatccattag tcggtctact gcgtgcagta tcaggaatat ggcggagccg gcgatgaagg
     3061 tcgcctgacc cagaccggcc agcgcctcgg ctttgccatg cccgaagcgg tgttcctggt
     3121 cggcgggcat gagcgagtag cgcacggcaa acagattgat cagcgatgct cccacgtcca
     3181 tgaccgaatc gaccagtgag gccaggacgc tcaccgaatc ggtggcgaac caggcggtca
     3241 gtttcgccgc gatgagcacc gtggctgtag ccacggatgc gtaggtcgca aagcgcagaa
     3301 gctgttcggt ggtcatggcg ggcatggcca ggcagatcgg gtggccggag atgttacacc
     3361 acgaccggtt gcgacggggc ctcgggcagg tgttttgacc atcctcttgc tcaatcagag
     3421 gaaccagttg gcaacggcac ggtgtaggcg ttgtggagcg ggggatctcg cgatagcctt
     3481 cgctcatgag cctcgtgaat gcccttgttg cgcatctgct ggtagcgctt atgctgacga
     3541 cgccgctcac ggcgttggct ggttcctgcg agggggccgg cgaagcacct gcgcgtgcag
     3601 ccgggcatca tgtgggtcat ggtcccgagc acgccggtta cgatgagcat catggcgacc
     3661 atggggaggt gtccgtcgaa gacggggccg cggactgtga ttgctgcgac gagtgtctcc
     3721 aggagtgtgc cgcctcggga tcgacgtcct ttctcgccgg gagtgctctg gaagcgccac
     3781 tgcgatccgg tccgaaacgg ttcgtcaccg actggctgag cggtcacgtc cgcgcgcctc
     3841 ccacgtcctt attccgtcct ccaatcctcg actgctgatc gagcggcgct gctggcgccc
     3901 cagttcccat ggcccgttgc ggtcttctgg gagtcggcat ggtgccgctc atttctcggt
     3961 catcagtgaa ctgaacgagg gtgtggcgga tgaaacgacg aatgatgagg acgtggctgg
     4021 tcgcgtccct ggcggttgcg ggtgttgccc aaggctccga ggacggcggc gatctgctcc
     4081 ggacgggttt gacgctggag ctgg
//
LOCUS       DBNHQR010000315         2113 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_980768, whole genome shotgun sequence.
ACCESSION   DBNHQR010000315 DBNHQR010000000
VERSION     DBNHQR010000315.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_980768"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="AC16VR_07055"
     CDS             <1..487
                     /locus_tag="AC16VR_07055"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6010952.1"
                     /translation="NRLLKVAYLETLRHSAPVLSAERFARHEVERFGKLIPEGAQLIC
                     SAAAANRDPRIFADPDRFIVDRRDMCQREPRGQYRADGLASGIAFGLGPPSRHPAIPE
                     DRPRSLYAITRDTAVRASRVLLDMVADLRLQEGANPHLASIRVGEMHTCWKLPVSFSK
                     R"
     gene            530..1810
                     /locus_tag="AC16VR_07060"
     CDS             530..1810
                     /locus_tag="AC16VR_07060"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6010953.1"
                     /translation="MSATAESLADLDPREFDSEAFQQDPFPIYKRLRDYHPVYHDRFH
                     NRWILSRYWDIDGAFQDNESFDRAVYKPDGPYEFGSRHVFGPNILEYGNSNEHRRLRN
                     IVAGQFVGQKLTDFLPMIDQISQEVIDRFIDDGEVELVTQFSSQVPIRVISNMLGLPR
                     EDEDTFVDWYQLLIAGLGFGGDHMARGIKARNDMWDYIDPMIRERSKNPGEDLLSRIC
                     VAELDGSRMSVEEIKGFVALLLAAGGDTTDKAIANMWYHMLYTRPDQLSDVLENPALW
                     ENVFTEMMRYDPVVHAQFRHTTREIPLHGHVIPERAGVILYLGAGNRDERVFKDPDTF
                     DIHRDDLHMGRENRSGRYKDGKPGHLGFGIGQHFCMGYAMARQESAIACTRLMEAIRN
                     PRPKFAEHPGISSPSIDSGGFRSPQELWMEFDRP"
     gene            1807..2001
                     /locus_tag="AC16VR_07065"
     CDS             1807..2001
                     /locus_tag="AC16VR_07065"
                     /inference="COORDINATES: protein motif:HMM:NF024851.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HIR6010954.1"
                     /translation="MKVKIDYEKCYKSGECYYNHPELFRMGDEGYPVVLVDQISDDAT
                     RRHAEEAIEVCPAVAITLED"
BASE COUNT          418 a          646 c          669 g          380 t
ORIGIN      
        1 caaccggttg ctcaaggttg cctacctgga gactctcagg cactcggcgc cggtgctttc
       61 ggccgagcgg tttgcccgcc acgaggtaga gcgttttggc aagctcatcc ccgagggtgc
      121 tcagctgatc tgcagcgctg cggcggcaaa tcgtgacccg cggatattcg ccgatccgga
      181 tcgtttcatc gtcgatcgtc gggacatgtg tcagcgcgaa ccgcgcggcc agtaccgagc
      241 cgacggcctg gcctcgggaa tcgccttcgg gctggggccg ccgtcgcggc atccggccat
      301 tcccgaggac cgccccagat cgctctacgc cattacccgg gacacggcgg taagggcctc
      361 ccgggttctg ctggacatgg tcgccgacct gcgcctccag gaaggcgcga atcctcatct
      421 ggccagtatc agggttgggg aaatgcatac gtgctggaag ctaccggtca gcttttcgaa
      481 gcggtagcct tcccgcgcaa cggaacacat cactaaacag gagggagaag tgtccgctac
      541 agctgaatca ctggcagacc tggatccccg ggaattcgac tcggaagcgt ttcagcagga
      601 tccgttcccc atctacaagc ggcttcggga ttaccacccg gtctatcacg atcgcttcca
      661 caatcggtgg atcctttccc gatactggga catcgacgga gcctttcagg acaacgagag
      721 cttcgaccgg gcggtctaca agcccgacgg gccttacgag ttcggctcca ggcacgtgtt
      781 cgggcccaac atcctcgaat acgggaacag caacgagcac cggcggctgc gcaacatcgt
      841 ggccgggcag ttcgtcggcc agaagctgac cgactttctg cccatgatcg accagatctc
      901 ccaggaggtc atcgaccgat tcattgacga tggcgaagtt gagctggtga cacagttttc
      961 cagccaggtg ccgatccggg tcatctccaa catgcttgga ttgccccggg aggacgagga
     1021 taccttcgtc gactggtatc agctgctgat cgccggactc ggcttcggcg gcgaccacat
     1081 ggcgcgaggg attaaggcgc gtaacgacat gtgggactac atcgacccga tgatccggga
     1141 gaggtcgaag aatcccgggg aagatctgct ctcgcggatc tgcgttgccg agctcgacgg
     1201 cagccgaatg agcgtggagg agatcaaagg gttcgtggcg ctgctgctgg ccgcaggggg
     1261 agacaccacg gacaaggcca tcgccaacat gtggtatcac atgctgtaca cgaggcccga
     1321 tcagctctcc gacgtgctcg aaaatccggc gctgtgggag aacgtgttca ccgagatgat
     1381 gcgctacgac cccgtggttc acgctcaatt ccggcacacg acccgggaga tcccgctgca
     1441 cggccacgtc attcctgagc gggccggggt gatcctctac ctcggtgcgg gcaaccggga
     1501 cgaacgggtg ttcaaggatc ccgacacgtt cgacattcat cgcgacgacc tccacatggg
     1561 gcgcgagaac cgttcggggc gttacaagga cggcaagcca gggcatctgg gtttcggcat
     1621 cggccagcat ttctgcatgg gctatgccat ggcgcggcag gaatcggcca tcgcgtgcac
     1681 caggctgatg gaagccatcc gcaacccgcg gccgaagttc gccgagcacc cggggatcag
     1741 ctcgccgtcc atcgattccg gcgggttccg gtcacctcag gaactgtgga tggagttcga
     1801 ccgcccatga aggtgaagat cgactacgag aagtgctaca agtcgggcga gtgctactac
     1861 aaccatcccg agctgttccg catgggggac gagggctacc cggtggtgct ggtggaccag
     1921 atcagcgatg acgccacgcg caggcacgcc gaggaagcaa tcgaggtctg cccggcggtg
     1981 gccatcacgc tggaagactg aaccccatca cctgtgttac gaggcaaaac gcccgacgcg
     2041 gtcgctgatg agcgcgtcgg gcgcggcttt ctggatgctt cagttcccga agttgtaggt
     2101 cagcgatccg tac
//
LOCUS       DBNHQR010000316         3414 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_791358, whole genome shotgun sequence.
ACCESSION   DBNHQR010000316 DBNHQR010000000
VERSION     DBNHQR010000316.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_791358"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="AC16VR_07070"
     CDS             complement(<1..535)
                     /locus_tag="AC16VR_07070"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024578.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR6010955.1"
                     /translation="MVLGIGVGIGAYRAWLEPDVNGRHGQAFDEVLRQVHEGYVDEVA
                     KEKLLENALKGMLHGLDEHSDYLDHLSYEDLQAETSGQFGGIGIELNLVDGYFTVIRP
                     ITDTPASAAGLQPGDRLTEIDRESLKGKKMVDIISSLRGTPGTPVILGIRRDGGQKME
                     VSLIRAVIQDPSVDGRLL"
     gene            complement(600..1703)
                     /locus_tag="AC16VR_07075"
     CDS             complement(600..1703)
                     /locus_tag="AC16VR_07075"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC family protein"
                     /protein_id="HIR6010956.1"
                     /translation="MVLSLLAPLSFSSEKEDAQRELEDVVGKLNALDVWLDEAEERRV
                     RWLGEVKIKDREVASVSEAVDEATAALAVVIRSLGKLEAEQRELQEKRERQATKIGEH
                     LAAAYRLDGRDFVQLLLNQESPDSFDRMIRYHRYFSSARLSALQEYQETLEALAANRL
                     ALEDRAAEQEARKAELAERQARLIEERDQRRLLIAQLDAEAEDKAVQRQRLDTDRDRL
                     ERLLDELNRRAMELDGSAFVARKGSLPWPLKGPLRSSFGRPRADGRMVWQGMLVATDE
                     GSPVKAVFRGRLIFADWLRGFGLLAIINHGDGYMTLYGHADSLTKTVGDWVEAGEVIA
                     RAGRSGGQQASGLYFEVREEGVPRDPLGWLAKR"
     gene            complement(1781..3334)
                     /gene="gpmI"
                     /locus_tag="AC16VR_07080"
     CDS             complement(1781..3334)
                     /gene="gpmI"
                     /locus_tag="AC16VR_07080"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008169322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HIR6010957.1"
                     /translation="MGNPTLLVVLDGFGYSPSTQDNAIAQARTPNWDRFWNEKPHALI
                     SGSGADVGLPDDQMGNSEVGHMNLGAGRKVYQDLTRINRAIEQGEFARNRTLNRTLQR
                     LRKAGGRLHVMGLLSPGGVHSHEDQIFEFIRMAAAAEIQHIYLHAFLDGRDTPPRSAE
                     PSLARADALLAELGAGHVASVTGRYYAMDRDRRWDRIERAYNLLVRGEAPFHAANGRA
                     ALAAAYERDESDEFVQPTAVHAEGELPVRMLDGDAVVFMNFRADRARQITRAILEPGF
                     DGFQRRSTVALSDFVMLTRYADDIDTSCAFEPAVLSNTLGEYLANLGKTQLRIAETEK
                     YAHVTFFFSGGREDPFAGEERLLIPSPKVATYDLAPEMSARKVTDHLVEAIASKQHDL
                     IICNFANGDMVGHTGVFDAAVKAVETLDECLGRIETAIVEAGGQMLITADHGNVEQMR
                     DHGTGQAHTAHTCEPVPLVYIGPQSVKLNSGGTLSDVAPTLLELMHLEKPAEMTGHSV
                     AQLEERRSA"
BASE COUNT          521 a         1123 c         1130 g          640 t
ORIGIN      
        1 cgagcagccg gccgtccacg ctgggatcct gtattacggc ccggatcagg ctgacctcca
       61 tcttctggcc gccgtcgcgg cgaatgccca ggatgaccgg cgtgccgggt gtcccgcgaa
      121 ggctggagat gatgtccacc atcttcttgc ccttgagcga ctctctgtcg atctcggtca
      181 gcctgtcgcc gggctggagc cccgcggcgc tggcgggcgt gtcggtgatc ggccggatta
      241 cggtgaaata gccgtcgacc aggttgagct cgatgccgat gccgccgaac tgcccgctgg
      301 tttccgcctg cagatcctcg tagctgaggt ggtccagata gtcggaatgc tcgtcgagac
      361 cgtggagcat tcccttgagg gcgttttcga ggagcttttc cttggccacc tcgtccacgt
      421 agccttcgtg cacctggcgc agcacctcgt cgaaggcctg gccgtgccgg ccattcacat
      481 cgggctccag ccaggcccgg taagcgccga tgccgacgcc gatcccgagg accatgctga
      541 tcgcgatgcc gaccagagtt atgggacgca agttcatacc ggggagtttg gcaccagcat
      601 cagcgtttgg ccaaccaccc gagggggtcg cggggcacgc cttcctcgcg cacctcgaag
      661 tacaggccgc tggcctgctg gccgccgctg cggccggcgc gggcgatgac ctcccccgcc
      721 tcgacccagt cgcccacggt tttcgtgagc gagtcggcat ggccgtatag cgtcatgtaa
      781 ccgtcgccgt ggttgatgat ggcgagcagt ccgaagccgc gcagccagtc ggcgaaaatc
      841 aaccggccgc gaaaaacggc ctttaccggc gagccttcgt ccgtggccac cagcatccct
      901 tgccagacca ttctcccgtc ggcccgtggc cgtccgaacg atgagcgcag gggacccttg
      961 agcggccagg gaagtgatcc cttgcgggca acgaaggcgg acccgtccag ctccatggcg
     1021 cgtcggttga gctcgtccag cagacgttcg agccggtcgc ggtcggtgtc cagccgctgc
     1081 cgctggacgg ctttgtcctc tgcctcggca tccagctgcg cgatcagaag cctgcgctga
     1141 tcgcgctcct cgatgagcct ggcctggcgc tccgcaagct ccgccttgcg ggcttcctgc
     1201 tcggccgcac gatcttccag ggccagcctg ttggcggcca gcgcctcgag agtttcctgg
     1261 tactcctgca gggcagacag gcgggcgctg ctgaagtagc ggtggtaacg gatcatccga
     1321 tcgaacgagt ccggggattc ctgattgagc aagagctgca cgaagtcgcg accgtccaaa
     1381 cggtacgcgg cggcgaggtg ctcgccgatc ttggttgcct gccgctcccg cttctcctgc
     1441 agctcgcgct gctccgcctc gagcttgccc agggaccgaa tcaccacggc caacgcggcg
     1501 gtcgcctcgt cgacggcctc gctcaccgag gccacctccc ggtccttgat cttgacctct
     1561 ccgagccagc gcacgcggcg ctcctcggct tcatcaagcc atacgtccag ggcgttgagc
     1621 ttgcccacga cgtcctccag ctctctctgg gcgtcttcct tctcgctgga gaagctcaac
     1681 ggcgccagga gagaaagcac cacgatcgca aggcgacgcg tgccggccgc ccggcgcccg
     1741 gcgcggctcc gcggtagcca gtcggtcgtc gggagactgt tcaagcggag cggcgttcct
     1801 ctagctgagc gacggagtgc ccggtcattt ctgccggttt ttccaggtgc atgagctcga
     1861 ggagggtggg tgctacgtcg gagagggttc ctccggagtt cagcttgacc gattgcggtc
     1921 cgatgtagac gaggggcacg ggctcacagg tgtgggcggt gtgagcctgg ccggtgccat
     1981 ggtcgcgcat ctgctccacg ttgccgtggt cggctgtgat gagcatctgc ccgcctgcct
     2041 ctacgatggc ggtttcgatc cggcccaggc actcgtccag tgtctccacg gccttcacgg
     2101 ccgcgtcgaa cacgccggtg tgaccgacca tgtcgccgtt ggcaaagttg cagatgatca
     2161 ggtcgtgctg cttcgacgct atcgcttcca ccaggtggtc ggtgaccttg cgcgcgctca
     2221 tctcgggcgc caggtcgtag gtggccactt tcggtgaagg gatgagcagg cgttcctcgc
     2281 cggcgaaggg atcctctctc ccgccggaga agaagaaagt gacgtgagcg tacttctcgg
     2341 tctcggcgat gcgcagctgg gtcttgccca ggttcgccag gtactcgccg agcgtgttgg
     2401 acagcacggc cggctcgaac gcgcacgagg tgtcgatgtc gtcggcgtac cgggtgagca
     2461 tgacgaagtc ggacagcgct accgtggagc ggcgctggaa accgtcgaag cccggctcca
     2521 ggattgcccg ggtgatctgg cgggctcggt cggcgcggaa gttcatgaag accacggcgt
     2581 ctccgtcgag catccggacc ggcagctcgc cctccgcgtg cacggccgtg ggctgcacga
     2641 actcgtcgct ttcgtcccgc tcgtacgcgg ccgccagcgc tgcgcgcccg tttgccgcat
     2701 gaaagggagc ctctccccgg accagcaggt tgtacgcgcg ctcgatgcgg tcccagcgcc
     2761 ggtctcggtc catggcgtag tagcgcccgg taacagaggc tacgtgacct gcgccgagct
     2821 cggcaagcag agcgtcagcc cgtgcgaggg acggctccgc gctgcgcggc ggtgtgtcgc
     2881 gcccgtccag aaaggcgtgc agatagatgt gctgaatttc cgccgccgcg gccatccgga
     2941 tgaactcgaa gatctgatct tcgtggctgt gcaccccgcc cggggacaac aggcccatga
     3001 cgtgcaggcg cccgccggct tttctgagac gctgcaaagt cctgttgaga gttctgttgc
     3061 gggcgaactc gccttgctcg atggcgcggt tgatccgggt caggtcctga tagaccttgc
     3121 gaccggcccc gaggttcatg tgccccactt ccgagttccc catctggtcg tccggaaggc
     3181 caacgtccgc ccccgagccc gagatcagcg catgaggttt ctcgttccag aagcgatccc
     3241 agttgggcgt gcgcgcctgg gcaatggcgt tgtcctgcgt ggacgggctg tagccgaaac
     3301 cgtccagaac caccagcagc gtcggatttc ccatgggttt tcggccaaac ctcctcgagt
     3361 tgcggggttg gggtagcggc cgggagttta acagaatcca tggctacgag aggt
//
LOCUS       DBNHQR010000317         2480 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_727267, whole genome shotgun sequence.
ACCESSION   DBNHQR010000317 DBNHQR010000000
VERSION     DBNHQR010000317.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_727267"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(193..1473)
                     /gene="purD"
                     /locus_tag="AC16VR_07085"
     CDS             complement(193..1473)
                     /gene="purD"
                     /locus_tag="AC16VR_07085"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009041976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIR6010958.1"
                     /translation="MNVLVIGSGGREHALAWKAAQSDGVETVFVAPGNAGTATEAGCE
                     NVDIAVDDFPALAEFAAANGVGLTIVGPEVPLVEGVTDYFEGRGLACFGPSKMAAQLE
                     GSKAFTKAFLDRHRIPTARYQAFTDLAAAQAYVREQGVPIVIKADGLAAGKGVVVAET
                     IEQALQALDDMLSGSAFGEAGSRVVIEEFLAGEEASFICISDGTNVVPFATSQDHKAR
                     DDGDKGPNTGGMGAYSPAPVVTAGVYDRIMKEVVNPTVRGMAEDGVPYVGFLYAGLMI
                     MPDGAPRVIEFNCRFGDPEAQPVMMRLRSDLVGLCRQALDGELAGVELDWDPRVALGV
                     VMAAGGYPEAYDKGDAITGIDTADADPRVKTFHAGTRLEDGVLVTNGGRVLCVVGLGD
                     DVAGARAAAYGAVEHIDWRNRYYRRDIGHRALDR"
     gene            complement(1510..>2480)
                     /gene="purH"
                     /locus_tag="AC16VR_07090"
     CDS             complement(1510..>2480)
                     /gene="purH"
                     /locus_tag="AC16VR_07090"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007945217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HIR6010959.1"
                     /translation="AEQEFPESLTLSYRHVETMRYGENPHQQAAFYRESGARPGTVAA
                     LRQLQGKSLSYNNVADTDAALECVKVFERPACVIVKHANPCGVAEAADVQAAYERAFA
                     TDPTSAFGGIIAFNRPLDEPTLTLILERQFVEVIVAPEAAPEAVAAAAARKNVRLLVT
                     GPLQAGSRGLELKRVGGGLLVQTPDETCLGEEGLRVVSRRAPSASELDDLLFAWKVAW
                     FVKSNAIVYARDGQTIGVGAGQMSRVVSARIAGFKAAEEGLEVAGSVMASDAFFPFRD
                     GIDAAAEAGIAAVVQPGGSVRDDEVIAAADEHGMAMVFTGIRHFRH"
BASE COUNT          413 a          885 c          787 g          395 t
ORIGIN      
        1 gcctgaccag ccaacggccc gcgggcaagc tcaaggtgct ggcgtgctcg gccaccggaa
       61 tgagcgccgc gctcgggagt cgagcaaatg gttcgaagcg gcgcagcgac cggacaatca
      121 tggcgccatt ttcccgcggc gcggagctgc ggcgccgccg ccttcagatt gttggcgtga
      181 cggaaattgc cgttatctgt cgagcgctcg atgcccgatg tcgcgccggt agtagcggtt
      241 ccgccagtcg atgtgctcga cggctccgta cgcggccgca cgggcgccgg caacgtcgtc
      301 cccaagcccc acgacgcaga gaacgcggcc accgttggtc accagcacgc cgtcttcgag
      361 acgggtgccg gcatgaaagg tcttcacgcg cggatctgcg tctgcggtat cgatgccggt
      421 aatggcgtca cccttgtcgt acgcctcggg gtaccccccc gcagccatca cgacgccgag
      481 cgccacccgc gggtcccagt cgagctcgac gccggcgagt tccccatcca gcgcctgccg
      541 gcagagaccg accagatcgg agcgcaggcg catcatcacg ggctgggctt ccggatcgcc
      601 gaaccggcag ttgaactcga tgaccctggg cgcaccgtcg ggcatgatca tgagcccggc
      661 gtagaggaag ccgacgtagg gaacgccgtc ctccgccatg ccacgcaccg tggggttgac
      721 cacctccttc atgatgcggt catagacgcc ggcagttacc acgggggcag gcgagtaggc
      781 gcccatgccg cccgtgttgg gccccttatc cccatcgtcc cgggctttgt gatcctgaga
      841 cgtggcgaac ggcaccacgt tggtgccgtc cgatatgcag atgaagctcg cctcctcgcc
      901 agccaggaac tcctcgatca ccacccggga tccggcctcg ccaaaagccg atccgctgag
      961 catgtcgtcc agcgcctgaa gggcctgctc gatggtttcc gcgaccacca ccccctttcc
     1021 ggccgccagg ccatccgcct tgatgacgat gggcacgccc tgctcgcgaa cgtaggcctg
     1081 agcggccgcc agatcggtga aggcttggta gcgcgcggtc gggatccggt gacggtccag
     1141 aaacgccttg gtgaaagcct tggagccttc cagctgggcc gccatcttgc tcggcccgaa
     1201 gcaggcgagc ccgcggccct cgaagtaatc ggtaacgcct tccacgaggg ggacttccgg
     1261 cccgacgatg gtgagcccta cgccgttcgc ggcggcaaac tctgccaggg cggggaaatc
     1321 gtccaccgcg atgtcgacgt tctcgcaccc ggcctccgtc gccgttcccg cgtttcccgg
     1381 tgccacgaag accgtctcca cgccgtcgct ctgagcggcc ttccaggcca gggcgtgctc
     1441 cctgccgccg ctgccaatga caagcacatt catctaggca tcctctaagg ttcgatggcc
     1501 ccgcaaaggc taatgccgga agtgacgtat gccggtgaac accatggcca tgccgtgctc
     1561 gtcggccgcc gcgatgacct cgtcgtcgcg aacggacccg cccggctgaa ccaccgccgc
     1621 gatcccggcc tcggcagccg cgtcgatgcc gtcccggaag ggaaagaagg catcggaagc
     1681 catcacggag ccggctacct ccagcccctc ctcggctgcc ttgaatcccg cgattcgggc
     1741 gctcaccacc ctgctcatct gacccgcgcc aacgccgatg gtctggccgt cgcgggcgta
     1801 gacgatggcg ttggacttga cgaaccatgc caccttccag gcgaacagca ggtcgtcgag
     1861 ctcgctggca gacggcgcgc gccgcgacac cacccgcagc ccctcttcac ccaggcaggt
     1921 ctcgtcgggc gtctggacga gcaacccgcc acccacgcgc ttcagctcga gcccgcggga
     1981 gcctgcctgc aaagggccgg tgacaagaag acgaacgttt ttccgggccg cagcagcggc
     2041 caccgcctcc ggcgccgcct cgggcgcgac gatgacctcc acgaactgac gctcgagaat
     2101 cagcgtcaac gtgggctcgt cgagcggtct gttgaaggcg atgatgccac cgaacgccga
     2161 ggtcggatcc gtcgcgaagg cacgttcgta tgcggcctgt acgtcggcgg cctccgcaac
     2221 gccgcacggg tttgcatgct tgacgatgac gcaggccggc cgctcgaaca ccttgacgca
     2281 ctccagcgcg gcatcggtat cggccacgtt gttgtaggac aggctcttgc cttgcagctg
     2341 gcgcagggcg gccacggtgc ccggtcgcgc ccccgactcg cggtaaaagg ccgcctgctg
     2401 atgaggattc tccccgtacc gcatggtctc cacgtggcga tagctgagcg tgagcgactc
     2461 cgggaactcc tgctccgcgg
//
LOCUS       DBNHQR010000318         7519 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1486674, whole genome shotgun sequence.
ACCESSION   DBNHQR010000318 DBNHQR010000000
VERSION     DBNHQR010000318.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7519)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7519)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7519
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1486674"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="AC16VR_07095"
     CDS             <1..695
                     /locus_tag="AC16VR_07095"
                     /inference="COORDINATES: protein motif:HMM:NF014874.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate ligase domain-containing protein"
                     /protein_id="HIR6010960.1"
                     /translation="DTVSVHDLPDHLSPVRFGRGDGSDVQLLEVRGQTADYLIGGEPF
                     TAHVPGGGEHRAASLAAVLATLKVLDRPVRDGLQLGDDLVPKGRGNETLINGVTVVDD
                     SYNANPESMKAALRTLAAVPGRKVAVLGDMLELGAEGPAHHRGLAEACSGIDRVYCVG
                     EAMRELFDALPEGQRGSWLPDAGGEGLIDEILADLRSGDRVLFKGSNRIFWAKGLVNR
                     FSERLHTFTMSD"
     gene            761..949
                     /locus_tag="AC16VR_07100"
     CDS             761..949
                     /locus_tag="AC16VR_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010961.1"
                     /translation="MANTHLNGRTFNYEGHCYLIVEDNDWEGSELKVKRLDGTRAQTS
                     LPTENVMRCVFPQKPDDR"
     gene            1219..2184
                     /locus_tag="AC16VR_07105"
     CDS             1219..2184
                     /locus_tag="AC16VR_07105"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010208395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990140 - molybdopterin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0061798 - GTP 3',8'-cyclase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HIR6010962.1"
                     /translation="MIVDALGRRFRNLRVSLTAACNYACSYCVPDGKRLQSAQHELDG
                     EELAYAVELVVDAAGIEQLRITGGEPLLSPKFDEVLPRLMALPLDDVSITTNGQLVPR
                     KIDLLLESGLKRINVSLDTLDPLRFRDIARGGDLETVLGGIGALAEGGLKVKINMVPM
                     RTANLDQLVPMLEFCLERGFELRYIELMNMGHLRQGNQYLRDFVGMEEILETIGKRYE
                     FTRTDAPYDSTAVRYEIPDRGFFGIIANETEPFCSSCTRLRLSSNGFIYGCLSNTARH
                     DVKPLLALPRHQAIAQLQGVLVEALADKQDLGFTGEVTVMKFIGG"
     gene            2242..2784
                     /locus_tag="AC16VR_07110"
     CDS             2242..2784
                     /locus_tag="AC16VR_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007253383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HIR6010963.1"
                     /translation="MTDTSSIFEAIVSAQRDRRLPPVASWNPARVGHIDIRIASDGTW
                     YHEGEPIRRKNMVDLFSTVLRRDSDGYCLVTPAEKLLITVEDAPFVAVDFEARGESGG
                     QDLVFVINTGDAVVADSAHPVRVEDHGGVPRPYVMVRDGLEALIARPVFYRLVELAVE
                     EAGELCLYSRGERFVLGGVS"
     gene            complement(2829..4483)
                     /locus_tag="AC16VR_07115"
                     /pseudo
     CDS             complement(2829..4483)
                     /locus_tag="AC16VR_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019395839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
     gene            4745..5497
                     /locus_tag="AC16VR_07120"
     CDS             4745..5497
                     /locus_tag="AC16VR_07120"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HIR6010964.1"
                     /translation="MKVLVPVKRVVDYNVKIRVKPDNTGVDLNNVKMSLNPFCEISVE
                     EAVRLKEAGTATEVVAVAVGGTVCQEQLRTCLALGADRAVLIETDQEPQPLGIAKALK
                     ALQERENADIVIFGKQSIDGDNAQTGQMFAALTGMPQGTFASELTVADGKAQIVREVD
                     GGLQTVSVTLPCVVTTDLRLNEPRYASLPNIMKAKKKPLDVVTPDELGVNVAPTVETL
                     SVKPPAERQAGIKVGSVEELVDKLKNEAKVIS"
     gene            5494..6423
                     /locus_tag="AC16VR_07125"
     CDS             5494..6423
                     /locus_tag="AC16VR_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010563760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HIR6010965.1"
                     /translation="MSVLVVADHDNAELKPVTLVVVAAATAIGGDIDILVAGEGCGAV
                     AETAAKIPGVNKVLLADNAAYGHQLAENVAPLVAEIGAGYSHILTAHTTVGKNFLPRV
                     AAVLDVAQISDIQGVVGPDTFERPIYAGNAVATVKSNDAIKVLSVRGTAFDPVAAEGG
                     SASIESVASAHDAGVSSFIGEELAKSERPELTAASIIISGGRGMQSGENFVLLEGIAD
                     KLGAAIGASRAAVDAGFVPNDMQVGQTGKIVAPDLYIAVGISGAIQHLAGMKDSKVIV
                     AINKDEDAPIFQVADYGLVADLFKALPELESQI"
     gene            6494..6931
                     /locus_tag="AC16VR_07130"
     CDS             6494..6931
                     /locus_tag="AC16VR_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010966.1"
                     /translation="MAVYELNNGQLLRVARSIDGKLEQRYFSLIGLNKTQQRGVRREA
                     KAQDQAWQEAQEKAKAKRVREASTDRRHSTGVRGINLVRRPSPAFRVQVQTNGRSHVR
                     EFSVKRLGAEKAWRQATRFLAECRGYKTVPKAWTASVPRIPKG"
     gene            complement(6928..>7519)
                     /locus_tag="AC16VR_07135"
     CDS             complement(6928..>7519)
                     /locus_tag="AC16VR_07135"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF012412.7"
                     /note="catalyzes the conversion of carbamoyl phosphate and
                     aspartate to form N-carbamoyl aspartate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HIR6010967.1"
                     /translation="LYTIRKEMESRGRAIDDLRIALVGDLRYGRAVHSLCKLLALFEH
                     VHYKLVSPPELSLPADIVASLRAAGHDVEEHHELETGIDHVDILYVTRTQEERFPDQE
                     EADRYRGLFRVNQAIYTKNCEPNTVIMHPLPRDSRAQAQELDDDLIENPNLAIFRQTD
                     NGVLIRMALFALVLDVAGQVHNHAQPVTWYTAKRFR"
BASE COUNT         1364 a         2334 c         2471 g         1350 t
ORIGIN      
        1 aagataccgt gtcagtccac gacctgcccg accatctctc gccggtgcgt tttggccgtg
       61 gagacgggtc tgatgtgcag ctgctcgagg tgcgcggaca gacggccgac tacctgatcg
      121 ggggcgagcc cttcaccgct cacgtgcccg gcggtggcga gcatcgcgcg gcgtcccttg
      181 ccgccgtgct ggccactctg aaagttctgg atcgcccggt gcgcgatggc ctgcagctgg
      241 gtgacgacct ggtgccgaag ggccgtggca acgaaacgct catcaacggc gtgacggtgg
      301 tcgacgacag ctacaacgcc aaccccgaga gcatgaaggc ggctctgcgt acgcttgccg
      361 ccgtacccgg tcgtaaggtc gccgtgcttg gcgacatgct cgagttgggt gccgaggggc
      421 cggcccatca ccgggggctc gctgaagcct gcagcggaat tgatcgcgtc tactgcgtgg
      481 gagaggccat gcgcgagctc ttcgatgcgc tgcccgaggg tcagcgtggc tcgtggctcc
      541 ccgacgccgg cggtgaaggg ctcatcgatg agatcctcgc ggacctcaga tccggcgacc
      601 gggtactctt caagggctcc aatcgcattt tctgggctaa ggggctcgtc aatcgtttca
      661 gcgaacggct gcacacgttt acaatgtcgg attaaacctc acacaactgg ccggaactgc
      721 tttgccatca tgggcgcagc agtaaccaag gggatcgacg atggcgaata cgcatctcaa
      781 cggccggact ttcaattacg agggccattg ttacctcatc gtcgaggaca acgactggga
      841 aggctctgag ctgaaggtca aacgcctgga cggcacccgc gcgcaaacca gcctgccgac
      901 ggagaacgtc atgcgctgcg tattccccca gaagccggac gatcgttgaa ggcaaaaaaa
      961 acccggcgcc tggggaggac gccgggtacg caccataaag gtccgtaggt cacacatact
     1021 ggaacggcaa ggccgttcca cagccgctgc cctggtcgtt ggggggggag acatggcagc
     1081 agccggggac aagtaaacca atcggctgct cagatttccg gaactggttc tcatttacgc
     1141 gcgcccgcta gcacatttgc ctgagcatgg cgccgtgtca tactccgccc caatccacgg
     1201 ggtccgcggg gccagcagat gatcgtagac gcgctggggc gtcgcttccg caatttgagg
     1261 gtcagcctca ccgccgcatg caactacgcc tgcagctact gcgttccgga cggaaaacgc
     1321 ctgcagtcgg cccagcacga gctggacggc gaggagctcg cgtacgcggt ggagctcgtg
     1381 gtcgatgcgg ccggcatcga gcagctgcgc atcaccggtg gagaaccgct gctctcgccc
     1441 aagttcgacg aggtgctgcc ccggctcatg gctctgccgc tggacgacgt ctcgatcacc
     1501 accaacggcc agctcgtgcc tcgcaagatc gacctgctgc tcgaatcggg cctcaagcgg
     1561 atcaacgtca gcctcgacac gctcgaccct ctccggtttc gggacattgc ccggggcggc
     1621 gacctcgaga ccgtgctcgg cggcatcgga gcgcttgccg agggtgggct caaggtcaag
     1681 atcaacatgg tcccaatgcg cacggccaat ctcgatcagt tggtgcctat gctggagttc
     1741 tgtctggagc gtgggttcga gctgcgctac atcgagctca tgaacatggg tcatcttcgc
     1801 cagggcaacc agtacctgcg ggatttcgtg gggatggagg agatactcga gaccattggc
     1861 aagcgctacg agttcacccg aaccgacgcc ccttacgact ccacggccgt gcgctacgag
     1921 atacccgatc ggggtttctt cgggatcatc gccaacgaga ccgagccgtt ctgcagctcc
     1981 tgtacccgcc tgcggctgtc gtccaacggc ttcatctacg ggtgcctctc caacaccgcc
     2041 cggcacgacg tgaagcccct gctggcgctg ccccgtcatc aggccatcgc gcagctgcag
     2101 ggcgtgctcg tggaggcatt ggccgacaag caggacctcg gcttcaccgg tgaggtcacc
     2161 gtgatgaaat tcatcggcgg gtgagcgggt aagaccgatc tgccggtcgg cgccccatca
     2221 aagcaagccg ttctttccga catgacggac accagcagca tcttcgaagc catcgtctcg
     2281 gcgcagcggg accgccggct gccgccggtg gcgagctgga atcctgcccg ggtgggccac
     2341 atcgacatcc gaattgcctc cgacgggacc tggtatcacg aaggcgagcc catccggcgc
     2401 aagaacatgg tggatctttt cagcaccgtg ctgcgccggg actccgacgg ctattgcctg
     2461 gtgaccccgg cggaaaagct cctgatcacg gtcgaggacg cgccgttcgt ggccgtggat
     2521 ttcgaagctc gaggggagag cggcgggcag gacctggtct tcgtcatcaa tacgggcgat
     2581 gccgtcgttg ccgacagcgc acatcccgtg cgcgtggagg accacggggg cgtgccaagg
     2641 ccctacgtga tggtgcgtga cgggctcgaa gcgctcatcg cccggccggt cttctaccgg
     2701 ctggtggagc ttgccgtgga ggaagccggc gagctctgtc tgtacagccg cggcgagcgg
     2761 ttcgtcctgg gcggcgtctc ctagccggcc ttcgtttctg accaggaccg gacaggccgg
     2821 cgagttccct acatattcgg gtagttgggc ccgccggtgc cttcggggac cacccagttg
     2881 atgttctggg ccgggtcctt gatgtcgcac gtcttgcagt gcacgcagtt ctgcgcgttg
     2941 atctggaatc tggcgccgcc gttttcctcc accacctcgt agacgcctgc agggcagtag
     3001 cgctgggcgg gctcgtcgta ggccggcagg ttctggctga tggggatggc cgggtcggcc
     3061 agcaccaggt ggcagggctg gtcttcctcg tgattggtgt tcgaaagaaa gacggaggac
     3121 agcttgtcga aggaaagcac gccgtcgggc tttgggtagt cgatccgcct gctttgggcg
     3181 gccggcttaa gcgtggcgtg gtcgggcgtg ggatccttca gagtgaacgg cagcctgccg
     3241 cgaaacaggg tctggtccac ccagatgaac gcggccccgg cgagggtgcc gaacttgtgg
     3301 atggcctgct gggaattccg cgccgctgaa agctcctcgt gcagccagga cgagtcgaac
     3361 ttctcctgga agctggacag gtcctcgccg cccgcggaac cgccggaaag ggactcgaag
     3421 acggcttcgg ccgagagcat gcccgatttc atggcggtgt ggctgccctt gatcttggtc
     3481 atgttgagaa agccggcatc gtcacccacc agcagcgcgc cgggcaccga cagcttgggc
     3541 agcgcggaca ggccgccctt gacgatggcc cgggcaccgt aggagacgcg cttgcccccg
     3601 ctgagggtct cggcgatgag cggatggtgc ttccagcgct ggaactcgtc gaacgggctc
     3661 acgtggggat tgctgtagga caggtccacg atcagcccga gagacacctg ctggttgggc
     3721 aggtggtaga ggtaggaccc ggtgaacatg cctttcaagt gcctgccagg ccagcccatg
     3781 gtgtgcacca ccttgcccgg cacgtgattc tcgggggtca cgtcccatag ttccttgaag
     3841 ccgatggcgt agtgctgggt ggccgaatcc cgctgcagat cgaacttggc catcagctgc
     3901 ttgccgagat ggccgggcag ccctcggcga agatggtgta cttgcccctg agctcgtatc
     3961 cgggcgcgta agttgccttg tgttcgccgt ccgcgccgac gcccatgtcg cccgtggcga
     4021 cgccggccac ggccccgtct tccccataga ggatctcggc ggcggcaaat ccgggaaaga
     4081 cgttcacgcc catgttctcg gcctgctccc cgagccagcg gcagaggttg cccagactga
     4141 tcgcgtggtt gccgtgattg tgggtgactt tgggcacggc gaagcccggc atcttcaggc
     4201 ggttttcctc gttgacgaga tagtagacct cgtcaagggt caccgggttg tccagcggcg
     4261 cgccacgctc ctcccagtcg ggaaacaact cgttcaacgc ggtgggctcg aaaacggcgc
     4321 cggaaagaat gtgcgccccg atctccgagc ctttctccac caggcagacg ctcagctcct
     4381 gacccgatga ttcggccagc tgcatcagcc ggcaggcagc tgccaggccg gagggaccac
     4441 cgcccacgat gacgacgtcg aaatccatgg actctctttc cacgatgact ctccactgct
     4501 ttgatttgcg ggtttgcgtc gcagcttcaa aggccccgac cgggcgggct gctgggctca
     4561 aatattagct caaacattat aggggagggc cttcacccgc gcaccggttc tgcgctgccg
     4621 atcctcgggc ccccttcgtc ttgacctcgg tgaggaccca agggagaata cgcgcccctc
     4681 cgggtagccg tcgaaacgtc ggcacctgct gttctgttta acttgttagc cgttcgagga
     4741 attcatgaag gtactggttc cggtaaaacg cgtggtcgac tacaacgtca agatccgggt
     4801 caagcccgac aacactggcg tcgacctgaa caacgtcaag atgtcgttga acccattctg
     4861 cgagatttcc gtggaagaag ccgtccgcct caaggaagcc ggaacggcta ccgaggtggt
     4921 cgccgtggcg gtcggtggca ccgtttgtca ggagcagctc agaacctgcc tggcgctcgg
     4981 ggccgatcgt gcggtgctca tcgagacgga tcaggagccg cagcctcttg gcattgccaa
     5041 ggcgctcaag gcgctgcagg agcgggaaaa cgccgacatc gtgatcttcg gcaagcagag
     5101 catcgatggt gacaacgccc agaccggcca gatgttcgcc gctctgaccg ggatgcctca
     5161 gggcacgttc gcctcggagc tcaccgtagc cgatggcaaa gcgcagatcg tgcgggaggt
     5221 ggatggcggt ctgcagacgg tctccgtcac gctgccctgc gtggtcacca cggacctgcg
     5281 gctgaacgag ccgcgctacg cgtctctccc caacatcatg aaggccaaga agaaacctct
     5341 ggacgtcgtc acgccggacg agctgggggt gaatgttgct cccaccgtgg agacgctttc
     5401 cgtcaaaccg ccggccgaac ggcaggcggg tatcaaggtg gggagcgtcg aggagctggt
     5461 cgacaagctg aagaacgagg caaaggtaat ctcatgagcg tattggtagt tgcagaccac
     5521 gacaacgcgg aactcaagcc ggtcaccctg gtggtggtgg cggccgcgac ggccatcggc
     5581 ggcgatatcg acatcctggt cgcaggtgaa ggctgcggcg cggtggctga aaccgccgcc
     5641 aagattcccg gggtcaacaa ggtgctcctg gcagacaatg ctgcctacgg gcatcagctt
     5701 gcggagaacg tcgcgccgct ggtggcggaa attggcgccg ggtactccca catcctcact
     5761 gcccatacca cggtcggcaa gaactttctg ccccgggtgg cagccgtgct cgacgtggcg
     5821 cagatatccg acatccaggg tgtggtcggg ccggatacct tcgagcggcc gatctacgcc
     5881 ggcaacgctg tggcgacggt gaagtccaac gacgccatca aggtgctgtc cgtgcgcggc
     5941 actgccttcg accccgtggc cgccgagggc ggctcggcca gcatcgagag cgtggcaagc
     6001 gcccacgatg ccggggtgtc cagctttatt ggagaagagc tggccaaatc ggagcgtcct
     6061 gagctgacgg cggcgagcat catcatctcc ggcgggcgcg gcatgcagag cggggagaac
     6121 ttcgtgctgc tcgaagggat agccgacaag ctcggcgctg ccatcggcgc gtcccgagcc
     6181 gcagtggacg ccggcttcgt ccccaacgac atgcaggtgg gccagaccgg caagatcgtg
     6241 gcacccgatc tctacattgc cgtgggcatt tccggcgcca tccagcatct tgcaggcatg
     6301 aaggacagca aggtgatcgt ggccatcaac aaggacgaag acgccccgat tttccaggtg
     6361 gccgactacg gcctggtggc cgatctcttc aaagcgctgc cggagctgga atcccagatc
     6421 tgacgcgcgt acagaaacat ttgacggcta cggaacctgt ctttgcgagg ttccttagcg
     6481 aggagttagt attttggcgg tttacgaact caacaacggc cagcttctgc gggttgcccg
     6541 cagcatcgac ggcaagctcg agcagcgtta cttcagcctc atcgggctga acaagaccca
     6601 gcagcggggc gtgcgtcgcg aggcgaaagc acaggaccag gcctggcagg aggctcagga
     6661 gaaggccaag gccaagcgcg tgcgggaggc ctctaccgat cgcaggcaca gcacgggcgt
     6721 gcgcggcatc aacctggtgc gcaggccctc acccgccttc cgggtgcagg ttcagaccaa
     6781 cggtcgctct cacgtccggg agttctcggt caagcggctc ggcgccgaga aggcctggcg
     6841 ccaggcgacc cgctttctag cggagtgccg cggctacaag accgtgccga aggcctggac
     6901 cgccagcgtg cctcgtatcc ccaaaggcta gcggaaccgc ttggccgtat accacgtcac
     6961 cggctgagcg tggttgtgca cctggccggc cacatccagc accagcgcaa acaaggccat
     7021 cctgatcaga accccgttgt ccgtctggcg gaaaatggcc agattggggt tttctatcag
     7081 atcgtcgtcc agctcctgag cctgggcgcg ggagtcccgt ggcagcggat gcatgatcac
     7141 cgtgttgggt tcgcagttct tcgtgtagat ggcctggttg acccggaaca gcccgcggta
     7201 gcggtcggct tcttcctggt cgggaaaccg ttcttcctgg gtgcgcgtca cgtagaggat
     7261 gtccacgtga tcgatgccgg tctccagctc gtggtgctcc tccacgtcgt ggccggccgc
     7321 tctgagcgac gccacgatgt ccgccggcag ggaaagttcg ggcggggaca ccagcttgta
     7381 gtggacgtgc tcgaaaagcg cgagcagctt gcagagggag tgcacggccc gtccgtagcg
     7441 caggtcaccc accagcgcga tccggagatc gtcgatggcg cggccgcggc tctccatttc
     7501 cttgcggatg gtgtagaga
//
LOCUS       DBNHQR010000319         4447 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1550463, whole genome shotgun sequence.
ACCESSION   DBNHQR010000319 DBNHQR010000000
VERSION     DBNHQR010000319.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1550463"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..726
                     /locus_tag="AC16VR_07140"
     CDS             76..726
                     /locus_tag="AC16VR_07140"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6010968.1"
                     /translation="MPATRSLTANQAQRRAKILQATRDLVTEHGYDGMIMRDVAALAD
                     VSAPTLYNLYNTKDELLLEALRDRIEASTVQARQHAPEPGYEYLLAHVRSVCRQTRRS
                     PAYVNAIAQALFRSTPGDPLAEVLLAGLQEDVERSLQAMSDAGHLHPQARINALARAL
                     TGSFWSNFLLWNKGRLALKDLETAQLSGYLSLLIPTTVGSANEALRSRLKALSGLS"
     gene            complement(813..1583)
                     /locus_tag="AC16VR_07145"
     CDS             complement(813..1583)
                     /locus_tag="AC16VR_07145"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6010969.1"
                     /translation="MNMHDKFRLDGQVAVVTGGGRGIGRGIALALADCGADVVVVARR
                     QADVEEVAEEIRERGRRGLGVSADVMDFTAIPGVLDRVVEEFGRLDIMVNNAGGNLDR
                     KTYSLPDIPIEKFDEQLTLNMKTKWWGAQQAAKRMENGGRIINIISVAAHRAAPGFGV
                     YSAANMGMISMTRTLAVELAPKKITVNCIAPGIIVTEMLKETMHISEEEAHQTFDAQI
                     PLGRTGTPEDCAAAAVFFASPAAEWTTGQFLDVAGGPA"
     gene            complement(1594..2916)
                     /locus_tag="AC16VR_07150"
     CDS             complement(1594..2916)
                     /locus_tag="AC16VR_07150"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6010970.1"
                     /translation="MGPRITGEDAVSQGQKTPYEPVVPEKTDDIDLLDVGLQNCPYHA
                     YRVLRDDAPVWQDPRTGFFVITRFEDLRQVLLDTEHFSNGANSNARDKLDSDRAMRMR
                     ELYETKGWVPAPTLAGRDDPNHKQMRSMFDEAFRPARINSMEPLVEGTARKLIDAFVD
                     DGHCDWVKQFAVPMPLIIIGVQMGAKEEDIWRIKAWTDAWVQRLGMMQTEEEERWSVE
                     MEIEAQNYFQPIFERLRKEPDDSLISDLVNRVIPEWGRPLNDNELHAEMMADTFVGGS
                     ETSTNALSYGVKLLIENPDSWDRLKSDPDKYLRTFCEEVLRLEGPVQGLFRRAAKDIE
                     LHGVQIPEGSMINIRYAAANRDEREFENPEKLDLDRKKPGRHLAFGSGIHHCLGAPLA
                     RRELYWGFKVLVERIDEMWFAPGKNDFTVAPNFSLRALKELHIEFNKA"
     gene            3038..>4447
                     /locus_tag="AC16VR_07155"
     CDS             3038..>4447
                     /locus_tag="AC16VR_07155"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6010971.1"
                     /translation="MAEPAKPERLKQSTLMLFGLPEYAVYLGSIPVGLYLPFVYSKDL
                     GLELVDVGLILMLARISDVITDPLIGYLSDRTGGRFGRRKPWLAAGALLMMVSAFQLF
                     NPNALSAAPVDNWHLLSWSVLLWLGWTMINIPYYAWGAELSDDYNERTRITGWRQAFG
                     FLGNVSVLAVPVIAGEITGYGSMPREGLTIIGSMALVALPTLIAVTLWRVPEREAYAK
                     PKASVLTHARAMSRNGSFILLFLGFMLMSLGTGWGSATFMLFATYVVGAEGQTQAILL
                     GYFGANVIALPLWVALAQRIGKKTTWLIGGVLFVIVTPCFLLLEHGDLWWFFLCLALY
                     GVAGGNFAALSMSMKADVIEIAARRSGENMAGSYIAVWSLGQKMVAALALGLALPFLQ
                     LLGFDPAGGNDAEQIRALALVYVVPPWLFYALAVVVIWRYPITASRLQRIRAAFERRD
                     ARLERKRIGRAGFGEQVEPT"
BASE COUNT          741 a         1507 c         1321 g          878 t
ORIGIN      
        1 acaaagaagc cgcgcgggca aagatcgatg ccttcgcggc cggtaactcg tccatcacct
       61 gaacggagcg agcccgtgcc tgcaacacgc tcgttgacgg ccaatcaggc ccagcggcgg
      121 gcgaagatcc tgcaggctac ccgggatctg gtaaccgagc acggctacga tggcatgatc
      181 atgcgggacg tggccgccct cgcagacgtc tcggctccga ccctttacaa cctctacaac
      241 accaaggacg agctgctttt ggaggccctg cgcgacagga tcgaagcaag caccgttcag
      301 gcccgtcagc acgctcccga accgggctac gagtacctcc tggcccacgt ccgcagcgtc
      361 tgccggcaga cccgccgctc ccctgcctac gtgaacgcga tcgcccaggc gctgtttcgc
      421 tccactccgg gagaccccct tgccgaggtg ctgctggcgg ggctgcagga ggacgtggag
      481 cggtcgctac aagcgatgag cgacgccggg cacctgcacc cccaagcgcg catcaacgcg
      541 ctcgcccgag ccctgacggg ttccttctgg tccaactttc tgctgtggaa caagggccgg
      601 ctcgcgctca aggacctgga aaccgcccag ctgagcggct acctttccct gctgatcccg
      661 accaccgtcg gctccgccaa cgaagccttg cgcagtcgcc tgaaggctct gagcggtctg
      721 agctgaaccc aagcggtcgg ctagacgcgg tcggctagac gcggtcggct agacgcggtc
      781 tgccagacgc ggtcggctag cccaggtcgg ccctaggcgg gcccccccgc gacgtctagg
      841 aactggccgg tggtccactc cgccgccggg gacgcaaaga acacggcagc ggctgcgcag
      901 tcctccggag tgcccgtccg cccgagcgga atctgcgcat cgaaagtctg gtgggcttcc
      961 tcctcggaaa tgtgcatggt ttccttgagc atctcggtga cgatgatgcc gggcgcgatg
     1021 cagttcacgg tgattttctt gggcgcaagc tccaccgcca gcgtccgggt catgctgatc
     1081 atccccatat tggccgcaga gtagacgccg aaccccggcg ccgctcgatg ggcggccacg
     1141 gaaatgatgt tgatgatgcg gccaccgttc tccattcgct tggccgcctg ctgagcaccc
     1201 caccacttgg tcttcatgtt cagggtgagc tgctcgtcga acttctctat gggtatgtcg
     1261 ggcagggagt acgttttccg atccagattc ccccccgcgt tgttcaccat gatgtccaga
     1321 cggccgaact cttccaccac ccggtccagg accccgggaa tggccgtgaa gtccatgacg
     1381 tccgcggaga cgcccagccc gcgccggccc cgctcgcgaa tctcctccgc cacctcttcg
     1441 acgtctgcct gacggcgggc gaccaccacc acatccgctc cgcagtcggc cagtgccagc
     1501 gcgattcccc gtccgatccc gcgtccacca ccggtgacca cggctacctg accatccagt
     1561 cggaacttgt cgtgcatgtt catttcgcgc ctcctaggcc ttgttgaact cgatgtgcag
     1621 ctccttgaga gcgcgcagag agaaattcgg tgcaacggtg aagtcgttct taccgggggc
     1681 aaaccacatc tcgtctatcc gctccacgag caccttgaaa ccccagtaga gctcgcgccg
     1741 cgccagcgga gccccgaggc agtggtgtat gccggaaccg aacgcgaggt ggcgcccggg
     1801 cttcttgcga tcgagatcga gcttctccgg attctcgaat tcccgctcgt cccggttcgc
     1861 agccgcgtat ctgatgttga tcattgatcc ttccggtatc tgcacgccgt gcagctctat
     1921 gtctttggct gctctccgga acaacccctg caccgggcct tcgagccgga gcacctcctc
     1981 gcagaacgta cgaaggtatt tatccggatc cgacttcagc cggtcccagg aatcggggtt
     2041 ctcgatgagc agcttcactc cgtaggacag cgcgttcgtg gaggtctccg agcctccgac
     2101 gaaggtatcg gccatcatct cggcgtgcag ctcattgtcg ttgagcggcc tgccccactc
     2161 gggaatgaca cggttcacga gatccgagat gagagaatcg tccggctcct tgcgaagtcg
     2221 ctcgaagatc ggctgaaagt agttctgcgc ctcgatctcc atttccacgg accagcgctc
     2281 ctcttcctcg gtctgcatca ttccgaggcg ctggacccag gcatccgtcc acgccttgat
     2341 ccgccagatg tcctcctcct tggcccccat ctgcacgccg atgatgatca gcggcatggg
     2401 gacggcgaac tgcttgaccc agtcgcaatg tccatcgtcg acaaaggcgt cgatgagctt
     2461 gcgcgccgtg ccctctacaa gcggttccat ggagtttatc cgcgccggcc tgaacgcttc
     2521 gtcgaacatg gaccgcatct gcttgtggtt cggatcgtcg cgaccggcca gggtcggcgc
     2581 cggaacccat cccttggttt cgtagagctc gcgcatccgc atggcccgat cggaatcgag
     2641 cttgtcgcgc gcgttgctgt ttgccccgtt cgagaagtgc tcggtatcca gcagaacctg
     2701 tcgcagatcc tcgaatcggg taatcacgaa aaagcccgtc ctcgggtcct gccacacggg
     2761 ggcgtcgtct ctcaagaccc gataggcgtg gtacggacaa ttctgcagac cgacatccag
     2821 caggtcgatg tcgtcggtct tctctggaac taccggctcg tatggtgtct tctgcccttg
     2881 tgacacggcg tcctcccctg tgatgcgcgg ccccatgggg ccattgtccg tcctggccat
     2941 ccagaacggc aatttattgt agcgctcacc agaaattttg ttgaccccac gaagcatcac
     3001 cccctagcct tggttcgtca gcttcgtgga gccaccggtg gccgagcccg caaaaccaga
     3061 gcgcctgaag cagtccacgc tgatgttgtt cgggctcccg gaatacgccg tctacctcgg
     3121 atcaatcccg gtcgggctgt atctgccttt cgtctactcc aaagacctcg gcctggagct
     3181 ggtcgacgtg ggcctcattc tgatgctggc gcgcatctcc gatgtgatca ccgacccgtt
     3241 gatcggctat ctcagcgacc gcacaggagg ccgcttcggc cgccgcaagc cctggctcgc
     3301 ggcgggcgcg ctcctgatga tggtctcggc attccagctc ttcaacccga acgcgctcag
     3361 cgcagcaccg gtggacaact ggcacctgtt gagctggagc gtgctgctct ggcttggctg
     3421 gaccatgatc aacatcccct actacgcctg gggcgccgag ctctccgacg actacaacga
     3481 acgcacccgc atcaccggat ggcggcaggc gttcggcttc ctcgggaacg tgagcgtcct
     3541 ggcagtgccc gtcattgccg gcgagatcac tggatacggc agcatgccca gagagggcct
     3601 gacgatcatc ggatccatgg cgctcgtggc gctgcccacg ttgatcgccg tgaccctctg
     3661 gcgcgtcccc gaacgcgagg cctacgcgaa gccgaaggca tcggtgctca cccacgcgcg
     3721 ggcgatgtca cgcaacggct ccttcatcct tctgttcctg ggattcatgc tcatgtccct
     3781 cggtaccggc tggggatcgg cgacgttcat gttgttcgcc acctacgtcg tcggggccga
     3841 gggacagact caggccattc tgctcggtta cttcggcgcg aacgttatcg ccctgcctct
     3901 gtgggtggcc ctcgcccagc gaatcggcaa gaaaaccacc tggctgatcg gaggggtgct
     3961 cttcgtcatc gtcaccccct gcttcctgct gctggagcac ggcgatctct ggtggttctt
     4021 cctttgcctg gcactctacg gggtggccgg gggcaatttc gccgcgctgt cgatgtccat
     4081 gaaggcagac gtgatcgaga tcgccgcgcg ccggtccggc gagaacatgg cgggttccta
     4141 catcgcggtc tggtccctcg gccagaagat ggtcgccgcc ctggctctgg gtctggcgct
     4201 accctttctg caactgctcg gtttcgaccc tgccggcggc aacgatgccg agcagatcag
     4261 ggccctcgcg ctcgtctacg tggttcctcc ctggctgttt tacgcgttgg cggtggtggt
     4321 gatatggcgc tacccgatca cggcctcccg cctgcaacga atccgggcgg ctttcgaacg
     4381 ccgcgacgcg cgcctggagc gtaagcgcat cggccgagct ggtttcggcg agcaggtcga
     4441 gcctacg
//
LOCUS       DBNHQR010000320         2619 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_601097, whole genome shotgun sequence.
ACCESSION   DBNHQR010000320 DBNHQR010000000
VERSION     DBNHQR010000320.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_601097"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /gene="dapA"
                     /locus_tag="AC16VR_07160"
     CDS             <1..597
                     /gene="dapA"
                     /locus_tag="AC16VR_07160"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HIR6010972.1"
                     /translation="KIGVDASLSVTPYYNRPTQEGLYRHYAAIAEAVDLPIVLYNVPP
                     RTGRDMTADTVARLAEIGNIVGIKEACGDPERVAEIRGLVAEDFFVLSGEDGQTLRMF
                     ELGAVGCISVTANVLPRRMAEFCRAWREGDVDLARAIDSELQPIHEILFVEPSPQPTK
                     WALYEMGMVDRGIRLPMLELSEENRPELIRRLKAVGAL"
     gene            615..1727
                     /gene="bamC"
                     /locus_tag="AC16VR_07165"
     CDS             615..1727
                     /gene="bamC"
                     /locus_tag="AC16VR_07165"
                     /inference="COORDINATES: protein motif:HMM:NF018509.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HIR6010973.1"
                     /translation="MRVARALLVVPLVAGCSWLNDDEGIFVNKSDDYLDAREGPGLYV
                     PEDLATGRVSDPFPIPPSPEQMNPEFYPQRPPQPSAIYASDTRDEVRIQRLGDRRWLA
                     LPESPTTVWPKLKQFLAENGVPIALEMGPEGRIDTDWLLISEDTYRDVVRLVIRDGRD
                     IAELTTGRDRIRFRLEQGMRERTCEVHIRHENDSLGLPAADSLVDIEGVASDIADIEN
                     DMLNEFGAYVAAKVSEQTVSMVAQEISAGAKSVLTRNEQGDPTLILYLDYERAWAALG
                     QAIARAEMDVTDQNRDEGTYYVNITEAFLAGEEKKGFFGRMFSFGGGEDAKELQIQME
                     RSGEEIYSVSVLDENAAPVDRELGQQLLIMIREFAS"
     gene            1724..2515
                     /locus_tag="AC16VR_07170"
     CDS             1724..2515
                     /locus_tag="AC16VR_07170"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6010974.1"
                     /translation="MTAFASLGSGSRGNGTLVDLGGELILVDCGFTLKQAENRFRRLD
                     VRPGDLSAILVTHEHADHAGGVAALSHKYAVPVFASHGTFKATRNELVGRDIDSHASF
                     DVGAVTVMPVIVPHDAREPTQFVFQHGAVRIGVISDLGQITPFVIEQYQGCDGLMMES
                     NHDREMLLRGRYPESVKRRIAGNLGHLSNEQAASFLEQVGHADLRVAVGHVSEENNHP
                     ELLEETFARFSGQVAELTFATQEDGLGWIRLPEGGVSAAAALGAG"
BASE COUNT          483 a          804 c          850 g          482 t
ORIGIN      
        1 aagatcggag tggacgcgag cctgtcggtc acgccctact acaaccgccc gacccaggaa
       61 ggtctttatc gtcactacgc agcgattgcc gaagcggtgg atcttcccat cgtgctctat
      121 aacgttccgc cacgcaccgg tcgcgacatg acggccgata cggtggcgcg tcttgccgaa
      181 atcggcaaca tcgtaggcat caaggaagcg tgcggcgacc cggagcgggt tgccgagatc
      241 cgcggtctcg tcgctgagga cttcttcgtt ctgtctggag aggatgggca gacgctgagg
      301 atgttcgaac tcggcgcggt gggttgcatc tcggtgaccg ccaacgtgct gccgcgcaga
      361 atggccgagt tctgccgtgc ctggcgtgag ggggacgtgg accttgcgcg ggccatcgat
      421 tcggagttgc agccgattca cgagatcctg ttcgtggagc cgagtcctca accgacgaag
      481 tgggcgctct acgagatggg tatggtggat cgcggcatac gtctgcctat gctggaactt
      541 tcagaggaaa accgtccgga gctgatccgg cgcctgaagg cggttggggc tctgtgatgc
      601 gcgcgcactg cctgatgaga gtcgcgagag cgctgctggt cgtgccgctc gtggcgggat
      661 gctcgtggct caacgacgac gagggcatct tcgtcaacaa gagcgatgac taccttgatg
      721 cccgggaggg ccccggactt tatgttcccg aggatctggc caccgggcgg gtgtccgacc
      781 cctttcccat cccgccgtct cccgagcaga tgaatccgga gttctacccg cagcgcccgc
      841 cgcagcccag cgccatttac gccagcgaca cccgtgacga agtccgcatt cagcggttgg
      901 gagacaggcg ctggctggcg ctgcccgagt cgcccaccac ggtatggccg aagctcaagc
      961 agttcctggc ggagaacggc gtgcccatcg cgctggagat gggtccggaa gggcgcatcg
     1021 acacggactg gctgctcatc agcgaggaca cttatcgtga cgtggttcgc cttgtcatcc
     1081 gcgacggccg ggacattgcc gagctgacca cgggccgcga ccgaatccgg ttccggctcg
     1141 agcaagggat gcgtgagcga acttgtgagg ttcacatccg tcatgagaac gacagcctgg
     1201 ggttgcctgc tgcagactct ctcgtggaca tcgagggcgt cgcctcagac atcgcggaca
     1261 tcgaaaacga catgctcaac gagttcggcg cttacgtcgc tgccaaagta tccgagcaga
     1321 ccgtttccat ggtggcacag gagatctcag cgggcgcgaa gtcggtgctc acccgtaacg
     1381 agcagggaga tccgaccctc atcctgtacc tcgactacga gcgggcttgg gccgccctgg
     1441 gtcaggccat cgcccgggcc gagatggacg tcaccgacca gaatcgcgac gagggcacct
     1501 actacgtgaa catcaccgaa gcgtttctgg ccggagaaga gaaaaaaggg ttcttcggcc
     1561 ggatgttctc gttcggcggt ggcgaagacg ccaaagaact tcagattcag atggagcgtt
     1621 ccggggagga gatctactcg gtttccgtcc tcgatgagaa cgcggctccc gtcgaccggg
     1681 agctggggca acagctgctg atcatgatcc gcgagtttgc cagttgacgg cttttgcctc
     1741 gctgggcagc ggtagccggg gcaacggtac gctcgtcgat ctgggtggag agctgatcct
     1801 cgtggactgc ggattcaccc tgaagcaggc cgagaaccgc tttcgccgtc tcgatgtccg
     1861 gcccggcgat ctctcggcga tcctggtcac ccacgagcac gctgaccacg ctggtggtgt
     1921 cgcggcgctc agccacaaat acgcggttcc cgtgtttgct tcccacggaa ccttcaaggc
     1981 cacccgcaat gagctcgtgg gcagggacat cgattcccat gccagcttcg acgtcggcgc
     2041 tgtgaccgtg atgccggtga tcgtacccca cgacgcccgg gagccgacgc agtttgtctt
     2101 ccagcacggt gccgtccgca tcggcgtcat ctccgacctc ggccagatta cccctttcgt
     2161 gatcgagcag tatcagggct gcgacggcct gatgatggaa agcaaccacg accgcgaaat
     2221 gctcctgcgc ggcaggtatc cggaaagcgt gaagcgccgc atcgccggca atctcggcca
     2281 tctatccaac gagcaggcag cgtcgttcct ggagcaggtg ggtcatgcgg acctgcgcgt
     2341 tgccgtcggc cacgtcagcg aggaaaacaa ccaccccgag ctgctggaag aaaccttcgc
     2401 ccggttcagc ggccaggttg ccgagctgac attcgccact caggaggatg ggcttggctg
     2461 gatccgcctg cccgagggcg gcgtcagcgc ggccgccgcc ttgggcgccg gctaatcgct
     2521 ggaaaacgtc cctcggtttg ctcagaatgt cgcccttgct gatctgacct cacggagagg
     2581 tgccggagct ggccgaacgg gcttgactcg aaatcaagt
//
LOCUS       DBNHQR010000321         4277 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1234713, whole genome shotgun sequence.
ACCESSION   DBNHQR010000321 DBNHQR010000000
VERSION     DBNHQR010000321.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4277)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1234713"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..1133)
                     /gene="lipA"
                     /locus_tag="AC16VR_07175"
     CDS             complement(141..1133)
                     /gene="lipA"
                     /locus_tag="AC16VR_07175"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HIR6010975.1"
                     /translation="MSKPVVSGKKFRTPEGFTAIKDGMKANGQAEPATGRKPPWLRVR
                     LGGGEKYQAVRRTVATHRLATVCEESHCPNIGECWNNGTATIMLMGSVCTRACRFCAV
                     DTGNPGGRLDPDEPANAAESVRLMGLRYVVLTSVDRDDLADGGAGHYAKCIQAIKAVN
                     PETAVEALTPDFSGSAEAVAVVVGSGLDVFAQNLETVERLTGDVRDPRAGYDQTLGVL
                     AEAKQVRRDLLTKSSLMLGLGEQDDEICQAMDDLREHGVDILTLGQYLRPTRNHLAVA
                     RWVHPDEFERYREWGLERGFTEVASGPLVRSSYRADRILEGNNLGLGRIPVKSI"
     gene            1182..2618
                     /locus_tag="AC16VR_07180"
     CDS             1182..2618
                     /locus_tag="AC16VR_07180"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6010976.1"
                     /translation="MQERPAVTRRVRWLYGAGAAAYGVKENGFSYFLLFYYNQVLDLP
                     GAYAGTAILIAMIFDAISDPLVGVWSDNTRSRWGRRHPFMYSAALPVAVIYFFLWNPP
                     ELTEFQLFVYLTVGCVLVRFFVTLYEIPSSAIVAELTDDYDERTRLLSYRYMMGWFGG
                     LGMAVLNWGLFMVVWGEAGETTFRVYGAVGAVIMFVAIVGSSLGLHRYIPYLHPPPER
                     TSYRVRQLTGDLIETISNRNFAALFFAGLFAAVGAGVATNFDTYIITHFWEFEAEQWR
                     WVIISLFVSALIPMYLAPLVTRRWDKKRSALGIYAFQIVFAALPILLRLAGWFPDNDS
                     AWTYPLIWAHAVVNVVVIVMFGVIQSSMLADVVEHSQASTGRREEGLFFAARSFAQKA
                     TSGVGAFIAGLALDWIAFPRGATPGTVPDAVIWDLGFIYGPALMIFYVLALVSIGFYR
                     ISRQGHDQRVGALRAGATAPSGSMEESR"
     gene            2615..3337
                     /locus_tag="AC16VR_07185"
     CDS             2615..3337
                     /locus_tag="AC16VR_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019689442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6010977.1"
                     /translation="MNDRPVIVTGGAGGIGSAIVARLVADGHPVAVLDLHPASATPSG
                     SLFSYPCDIADLEAVERAVAEFEAAVGAPFGLVNNAGWDQALPFLETGPDLWEKVIDV
                     NLRGPVNVTYAVLRRLAGAGRGRVVSIASDAGRVGSSGEAVYSACKGGIIAFMKTLAR
                     EHARQGITFNSVCPGPTDTPLLDSFDESGRLAQALERAIPMRRLGRPEDFPGIVAFLL
                     SDEASFITGQTVSVSGGLSMQG"
     gene            complement(3339..>4277)
                     /locus_tag="AC16VR_07190"
     CDS             complement(3339..>4277)
                     /locus_tag="AC16VR_07190"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6010978.1"
                     /translation="AVQEEMTLGAVILFTRFIDMLVHPIVVLGEQTNILFRAMASGER
                     IFQALDWDEKIHEPADPVTLPPRLQGEVTFRHLNFAYDPDVQILHDVSFEIGAGEKLA
                     IVGPTGSGKSTLIRLLGRFYEFDDGQIFLDGIDLNRIHTRDLRQRIGVVLQDFHIFSG
                     TVFDNIALGDPRITRERAEEAARRVNAHGFISDLPRGYDTELSERGQNLSQGQRQLLA
                     FARVLAADPEILVLDEATASIDTETELLIQDALRKLTAGRTSIIIAHRLQTIQEADRV
                     LVLQGGRVVELGTHEELIASEGLYHTLHSLQFQDEG"
BASE COUNT          638 a         1379 c         1431 g          829 t
ORIGIN      
        1 cctgctcgct ggatcaccct tttgcgctcg ccaactatct ggcgcggggc ttttccctgt
       61 tcgacatcgt cacgaccacg aaaaatctgc ccgatcccat gccggagccc cggcccgggg
      121 atgccgaggc gggccggtcg tcagattgat ttgaccggga tccgcccgag cccgaggttg
      181 ttgccttcga gtatgcggtc ggcccggtag ctcgacctta ccagcgggcc cgacgcgact
      241 tccgtgaagc cacgctccaa gccccattcc cgataccgtt cgaactcgtc cgggtgcacc
      301 cagcgcgcca ctgccagatg gttgcgggtg ggacgcaggt actggcccag ggtgaggatg
      361 tcgacgccgt gctcgcgcag atcgtccatg gcctggcaaa tctcgtcatc ctgctctccc
      421 aggccgagca tgaggctcga tttggtgagc aagtcccgac ggacctgctt ggcctcggcg
      481 agaacgccca gcgtctgatc gtatcccgcc cggggatcgc gaacgtcgcc ggtcaggcgc
      541 tccacggttt ccagattctg ggcgaagacg tccagccccg agcccaccac gacggcaacg
      601 gcctcggcgg atccgctgaa atccggcgtg agcgcctcga cggcggtctc ggggttgacc
      661 gccttgatgg cctggatgca cttcgcgtag tggccggcgc cgccgtccgc caggtcgtcc
      721 cggtcgacgg aggtcagcac gacgtagcgc agccccatca gacgcacgga ctccgctgcg
      781 tttgcgggct cgtccggatc gagacgccct ccgggattgc ccgtgtcaac cgcgcagaac
      841 cggcaggccc gggtgcacac ggagcccatc agcatgatgg tcgccgtgcc gttgttccag
      901 cactcaccga tgttcgggca gtgagattcc tcgcacacgg tggccagacg gtgggtggcc
      961 accgtcctgc gcactgcctg gtacttttcg ccgccgccga gccggacgcg cagccacggg
     1021 ggcttgcggc cggtagccgg ctctgcctgc ccgttcgcct tcatgccgtc cttgatggcg
     1081 gtgaaacctt cgggcgtgcg gaatttcttg ccgctgacga ctggtttgct catggcccgt
     1141 cattgtaccc ataatgccgc gtcgttgagg tgcggtaacc aatgcaggaa cgtccggcag
     1201 tcacccggcg ggtgcgatgg ctatacgggg ccggagccgc cgcctacggc gtgaaggaaa
     1261 acggcttctc ctatttcctg ctcttctact acaaccaggt gctcgatctt ccgggcgcct
     1321 atgccggcac ggcgatcctg atcgccatga tcttcgacgc catctcggac cctctggtgg
     1381 gcgtctggtc ggacaatacc aggtcgcgct ggggacgccg ccaccccttc atgtacagcg
     1441 ccgccctgcc cgtggcggtg atctacttct ttctctggaa cccgccggag ctaacggagt
     1501 ttcagctctt tgtctatctc accgtagggt gcgtgctcgt gcgtttcttt gtcacgctgt
     1561 acgagatacc gtccagcgcc atcgtcgcag agctcacgga cgactacgac gaacggaccc
     1621 ggttgctcag ctaccgctac atgatgggct ggttcggcgg tctcgggatg gcggtgctga
     1681 actggggttt gttcatggtg gtctggggag aggcgggcga aacgacgttc cgcgtctacg
     1741 gagcggtggg tgcggtgatc atgttcgtgg ccatcgtggg atcctccctc ggcctgcacc
     1801 gctacatacc ctacctgcac ccgccgccgg aaaggacctc ctaccgggtc cggcagctta
     1861 ccggcgatct catcgagacc atcagcaaca ggaacttcgc cgcgctgttc ttcgccggcc
     1921 tcttcgccgc ggtgggcgcc ggcgtggcga cgaatttcga cacctacatc atcacccact
     1981 tctgggagtt cgaggccgag cagtggcgct gggtgatcat ctcgttgttc gtctcggcac
     2041 tcattcccat gtatctggcg ccgctggtta cccggcgctg ggacaagaag cgaagcgcgc
     2101 tgggtatcta cgctttccag atcgtctttg ccgccctgcc tatcctgttg cgcctggccg
     2161 gctggtttcc ggataacgac agcgcctgga cctacccgct gatctgggct cacgccgtcg
     2221 tcaacgtggt cgtgatcgtg atgttcggcg tcatccagag ctcgatgctg gcggacgtgg
     2281 tggagcacag ccaggcgtcc accggccggc gtgaggaggg gctgttcttc gccgcgcgat
     2341 cgtttgccca gaaggcgacc tcgggggtcg gcgcgttcat cgccgggctg gcgctggact
     2401 ggatcgcgtt cccgaggggc gcgacgccgg ggacggtgcc ggacgccgtc atatgggatc
     2461 ttggtttcat ttacggcccc gcgctcatga ttttctacgt gctggcgttg gtttccatcg
     2521 ggttctatag gatctcgcgg cagggacacg atcagcgggt gggcgcgctg cgcgcgggcg
     2581 ccacggcccc gtcgggaagc atggaggaat cgcgatgaac gaccgacccg tgatcgtcac
     2641 cgggggcgcc gggggcatcg gcagcgccat cgttgcccgt cttgttgccg acggccatcc
     2701 ggtggccgtg ctcgatctgc accccgcgtc tgctacccct tcgggctccc tgttttccta
     2761 cccgtgcgac atcgccgatc tcgaggcggt ggagcgggcg gtcgccgagt tcgaggcggc
     2821 ggtgggcgcg ccgttcgggc tcgtcaacaa tgccggctgg gaccaggccc tgccgtttct
     2881 ggagacgggg ccggatctgt gggagaaggt gatcgacgtg aacctgcgcg gccccgtgaa
     2941 cgtcacctac gcggtgctgc gcaggctggc gggcgcgggc cgcggccggg tggtcagcat
     3001 cgcctcggat gccggccgtg tgggctcgtc gggagaggcc gtctactcag cgtgcaaagg
     3061 cggcatcatc gccttcatga agacgctggc tcgtgagcac gcgcgtcagg gcatcacgtt
     3121 caactccgtc tgccctggcc ccacggacac gcctctgctc gattccttcg acgagtcggg
     3181 tcggctggcc caggcgctcg agcgggccat acccatgcgg cggctgggaa gaccggagga
     3241 ctttccgggg atcgttgcct tcctgttgag tgacgaggcg tcgttcatca ccggccagac
     3301 cgtgagcgtc tctggcgggt tgtcgatgca ggggtgacct acccctcgtc ctgaaactgc
     3361 agcgagtgca gggtgtgata gagcccctcg ctggcgatca gctcctcatg ggtgccgagc
     3421 tcgaccaccc ttcctccctg aagaaccagc acccggtcgg cttcctgaat ggtctgcagg
     3481 cgatgggcga tgatgatcga agtccgcccg gcggtgagct ttcgcagcgc gtcctggatg
     3541 aggagctcgg tttcggtgtc gatgctcgcg gttgcctcgt cgagcacgag gatctcggga
     3601 tccgcggcca gcacccgggc gaaggccagc agctgccgct gcccctggga caggttctga
     3661 ccgcgctcgg agagctcggt gtcgtatccg cggggcaggt cgctgatgaa gccgtgggcg
     3721 ttgacgcggc gcgcggcctc ctcggcgcgc tcccgggtga tccggggatc gccgagggcg
     3781 atgttgtcga agacggtccc tgagaagatg tggaagtcct ggagcaccac gccgatgcgc
     3841 tggcgcaggt cgcgcgtatg gatgcggttg agatcgatgc cgtcgaggaa aatctggccg
     3901 tcgtcgaatt cgtagaacct tccgagcagg cggatcagcg tgctcttgcc ggaccccgtc
     3961 gggcccacga tggcgagctt ctccccggcc ccgatctcga aggagacgtc gtggagaatc
     4021 tgaacgtccg ggtcgtaggc gaagttcagg tgcctgaagg tgacctcgcc ctgcagccgg
     4081 ggcggcagcg tgacgggatc ggccggctcg tgaatcttct cgtcccagtc cagcgcctga
     4141 aagatccgct cgccgctcgc catggcccgg aagagaatgt tcgtctgctc gccgagaacc
     4201 acgatgggat ggacgagcat gtcgatgaag cgggtgaaca gaatcacggc gcccagggtc
     4261 atttcttcct gcactgc
//
LOCUS       DBNHQR010000322         6020 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1107766, whole genome shotgun sequence.
ACCESSION   DBNHQR010000322 DBNHQR010000000
VERSION     DBNHQR010000322.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6020)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6020)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6020
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1107766"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="AC16VR_07195"
     CDS             <1..476
                     /locus_tag="AC16VR_07195"
                     /inference="COORDINATES: protein motif:HMM:NF013488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HIR6010979.1"
                     /translation="YEGFAQHVREVAIRYEAPAVHEYLWTKVRPVTSGTAHLVLKATE
                     IAESPLEMTRLSRALREAFFVDARDVGDLGVCLEVAQGLGLAAAELKRHVDSGAAMAA
                     LLADYRKAEERGIRGSPSWVMNEGRQVLYGNVGYRILNANIEELLRHPEQEASWC"
     gene            574..1527
                     /locus_tag="AC16VR_07200"
     CDS             574..1527
                     /locus_tag="AC16VR_07200"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6010980.1"
                     /translation="MEFREIENGEIVLRVALSGHGPVILCVHGWPELWYSWRHQLRHF
                     AERGYTVAAMDVRGYGGSSKPHAIEAYRMTELCADVAAVARELSDEPVVLFGHDWGAP
                     IVWNTALRHPESVRAVAGLSVPYLPAGDVGTLDLFRGIYAGRFFYQLYFQEEGVVEAE
                     VEGDFETALRTIYFSLSGDAPLDDWLKNKPADASLLDGMVDPRPFPEWMSEEDLGVYV
                     DAFTAGGFRGPINRYRAQNLDVAESGPLRGLPVEQPACFVGGERDAVRHFLPGMDLYA
                     DPGAACADFRGSTIIAGAGHWVQQEAPEATNEALQRFLDGL"
     gene            complement(1628..2551)
                     /locus_tag="AC16VR_07205"
     CDS             complement(1628..2551)
                     /locus_tag="AC16VR_07205"
                     /inference="COORDINATES: protein motif:HMM:NF019896.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase family
                     metallohydrolase"
                     /protein_id="HIR6010981.1"
                     /translation="MKWLICLLVAGFSLPAAAYREATVPFGVKVQGIENRFAEFGVYA
                     RPGEILALDLIDVAPEEIGATFDSAPVKTGHGRINLKMPGAPGTHRVELTDQRSGSRM
                     RLNVFVMWQHDGARDDVLNGYRIGQYPRDPFRGLAVYRPPEAFVEVTPDNAHTRVSPN
                     FTLGQFVCKQGSGFPKYVVLRPQLVQKLELVLGELNAVNPDVTGLTVMSGYRTPAYNR
                     SLGNGRHSRHLWGGAADVYVDANGDGIMDDLNGDGRRDRGDAEWLATFIDRLEHAGRF
                     GEHIGGIGTYNSNAHHGPFVHVDARGYRARW"
     gene            2725..4368
                     /locus_tag="AC16VR_07210"
     CDS             2725..4368
                     /locus_tag="AC16VR_07210"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR6010982.1"
                     /translation="MLRVLRFSLPVAVAVLAFAVPLRVGAEVVAFELREFFDDYYSSG
                     AMAVQGQALLTERVLAEFYARRGYRPAWGDPALVAELFDLLARSVDDGLDPDDYNLTR
                     LRALRERAASSPKAVPERDLELLYTSSLLRFAYHQRFGKVNPKTLVSTWNFGRQLAPG
                     VDAADTMNQIVESGSLREGLAARVPRGPLYRNIQMALARYRSAAAAGGWEPVPEGVTL
                     TIGMRDERVVSVRRRLAVTGDLETGAVSDPELFDADLESGVRRFQHRHSLSVDGAVGP
                     ATVRAMNVSVEERIDQLRLSLERARWIMTARDTSVVVVNIAAAEVYVLEEGEMVWQRR
                     AMVGRTYRQTPVFRGLMTYLEINPTWTVPPGILRADILPKVREDPGYLARSNMTLLDR
                     SGRAVDPYAVDWQALEGMPYIFRQEPGPTNALGLIKFMFPNEHFVFIHDTPNRELFER
                     AERTFSSGCIRVEDPFSLAALLLRDPANWDEAAVRAAVETGETRRVRLADPWPVYILY
                     WTAQVDDDGVVRFYPDVYQRDVELLGELDGDVRIDLPAS"
     gene            4437..5960
                     /locus_tag="AC16VR_07215"
     CDS             4437..5960
                     /locus_tag="AC16VR_07215"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF039907.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HIR6010983.1"
                     /translation="MRADRRDFLKKTGSAVAAASTASLWPDLLLAEEVPTTAQALFET
                     RFGVSRGDMGRLLSLALGKGGEFAELFFEYKIANGVTMEEDIVKESSERVSLGVGIRV
                     LKGEQTGYGYTSDLTFEGMSQAARTAAAIASEGGRVEIAPLRLRANALQVYDLERPFA
                     ESSLSSRMIILRQTYDAAQAYHPKIDKVRVSLRDEIQYVLVANSEGLLASDTRPQVRL
                     IASVTAADGGNRSTGLSTAGGRVGSAFFSSARTPKELGREAAEEAIVLLGAVSPRPGD
                     QTVVLDSGQSGVMIHEAVGHPLEADSNRKKRSVFWDKLNQTVANPLVTIYDDPTIAHF
                     RGSHNMDDEGTLSRKTMLIEKGKLVGYLQDRLSAKLMKMSPSGHGRRDGFENVPLPRM
                     ANTNLARGESDPEQIVRSVKRGFFAKTYQGGMVQPSGKFTFSVNMGYLIEDGKLTAPV
                     KNATLIGSILDVLPEVTMVGNDMGTFLGTCGKGGQSAPVTAGTPTLKINSMTVGGRS"
BASE COUNT         1000 a         1872 c         2071 g         1077 t
ORIGIN      
        1 gctacgaagg atttgcgcag cacgtgcgcg aggtcgccat ccgctacgag gcgcctgccg
       61 tccacgagta tctctggacg aaggtcaggc cggtgacgtc gggcaccgcc catctggtgc
      121 tcaaggcaac ggagattgcg gagagccccc tggagatgac tcgcctgtct cgggccctgc
      181 gcgaggcgtt cttcgtcgat gctcgagacg tcggcgatct cggcgtctgc ctggaggttg
      241 ctcaggggct tggtctcgcc gccgcggaac tgaagcgaca cgtggattct ggcgcggcca
      301 tggccgccct tctggcggac tatcgcaagg cggaggagcg gggcatcagg ggaagcccgt
      361 cctgggtgat gaacgagggt cgccaggtgt tgtacggcaa cgtcggctac cgcattctga
      421 atgcaaatat cgaggagctg ctgcgccacc cggaacagga agccagctgg tgctaggttg
      481 ccgcgcgtcc ggcgcccggc cacaggcgcc gaagaggggg tgctgcgaac aggggctcgg
      541 cggggaaccg gccagctgat cgggggaggg ggaatggaat tccgggagat cgaaaacggc
      601 gagatcgtgc ttcgcgtcgc gctttccggg cacgggccgg tgatactttg cgtgcacggc
      661 tggcctgagc tctggtactc gtggcgccat cagctgaggc actttgccga gcgcggatac
      721 accgtagctg ccatggacgt gcgcggttac ggcggcagct ccaaacctca cgccatcgaa
      781 gcctaccgga tgaccgagct ttgcgcggac gtggcggccg tggcgcgaga gctcagcgac
      841 gagcccgtcg tgctgttcgg tcacgactgg ggcgccccca tcgtctggaa cacggcgctg
      901 cggcatccgg agtccgtgcg cgccgtggcg ggcttgagcg tgccctacct gccggccggc
      961 gacgttggca ccctggatct gttccgcggg atatacgccg gccggttctt ctaccagctc
     1021 tactttcagg aggagggcgt cgtggaggcc gaggtggaag gggacttcga gaccgcgctg
     1081 cgcacgatct acttctcgct gtctggtgat gcgccgctgg acgattggtt gaaaaacaag
     1141 ccggccgacg cctcactgct ggatgggatg gtcgatcccc gtcccttccc cgaatggatg
     1201 agcgaggaag acctgggggt ctacgtggac gcgtttaccg cgggcggttt ccgggggccc
     1261 atcaaccgct atcgcgcgca gaacctcgac gttgccgagt cggggccgtt aagggggctg
     1321 cccgtggagc agcctgcgtg cttcgttggc ggcgagcgcg acgcggtgcg gcactttctg
     1381 cccggcatgg atctctacgc ggatccgggc gctgcgtgcg ccgatttccg cggatcgacg
     1441 atcatcgccg gtgccgggca ttgggttcag caggaggcac cggaggcgac gaacgaggct
     1501 ctgcagcgtt tcctggatgg tctgtagctc tgagtcgtga gccaagcggc cgtcaattcg
     1561 gacgcttgat gcgggcccac gttcgggcgg cggcgctcag gccaggcagg ccgtcgcgtt
     1621 cgccgcttca ccaccgagcc cggtaaccgc gcgcgtcgac gtggacgaag gggccgtggt
     1681 gggcgttgct gttgtaggtg ccgatgccgc cgatgtgctc gccgaagcgc cctgcgtgct
     1741 ccagtcggtc gatgaaggtc gccagccact ctgcgtcgcc gcggtcgcgc cggccatcgc
     1801 cgttgagatc gtccatgatg ccgtcgccgt tggcgtccac gtagacgtcg gcggcgccgc
     1861 cccaaaggtg ccggctgtgc cggccgttgc ccagggatct gttgtaggcc ggggtgcggt
     1921 agccgctcat caccgtcagg cccgtgacgt ccgggttgac ggcgttcagc tcaccgagca
     1981 cgagctccag cttctgcacc agctgtgggc gcaggaccac gtacttgggg aatcccgaac
     2041 cctgcttgca gacgaactgc cccagggtga agttcggcga gacgcgcgtg tgcgcgttgt
     2101 cgggggtgac ctcgacgaac gcctcgggcg ggcggtagac ggccaggccg cggaaaggat
     2161 ccctggggta ctgaccgatg cggtaaccgt tgagcacgtc gtcacgcgcc ccgtcgtgtt
     2221 gccacatcac gaagacgttg agccgcatcc tggacccgct gcgctggtcg gtcagctcca
     2281 cccgatgggt tcccggcgcg cctggcattt tgaggttgat ccggccgtga cccgttttaa
     2341 cgggggccga gtcgaaggtt gcgccgatct cttcgggggc gacgtcgata aggtctaaag
     2401 ccaggatctc tccaggccgc gcgtacacgc cgaattcggc gaatcggttc tcgattccct
     2461 gaaccttcac gccgaaaggt accgtggctt cacgataggc ggcggccgga aggctaaatc
     2521 cggcaacaag caggcagatc agccatttca tcggggagcg tctctgtttt tcgggagttt
     2581 ggacggactc acgccctgga ttgtccaaca atataggatc cgtttggcgc tctaaagttc
     2641 ccggcgcccg cccggcaagc ctgcagtttg tactgctgtg tggagattgc taccttggct
     2701 cgcccgttga tccggcccgg aatcatgctt cgagtgctcc gtttcagcct gccggtggca
     2761 gtcgccgttc ttgccttcgc cgtgccgctt cgcgtcggcg cggaggtggt cgccttcgag
     2821 ctgcgggaat ttttcgacga ctactacagc agcggtgcca tggcggtcca ggggcaggcc
     2881 ctgctgaccg agagggtgct ggccgagttt tacgctcgtc ggggctatcg gccggcgtgg
     2941 ggtgacccgg cgctcgtcgc cgagctgttc gatctcctgg cccggagtgt ggacgacggc
     3001 ctcgatcccg acgactacaa cctcacgcgt ttgcgcgcct tgcgcgaacg ggcggcatcg
     3061 agcccgaagg cagtcccgga gcgcgacctc gagcttctgt acacctcaag cctgttgcgt
     3121 ttcgcctatc accagcggtt cggcaaggtc aatccgaaga cgctcgtgag cacgtggaac
     3181 tttggccggc agctcgcgcc gggcgtcgat gctgcggaca ccatgaacca gatcgtggag
     3241 tcggggagtc ttcgcgaagg gctggctgct cgcgtccccc gtggccccct ctaccgtaac
     3301 atccagatgg cgctcgcgag gtaccggtcc gccgccgcgg cggggggatg ggagcccgtc
     3361 cccgagggcg tcacattgac catcggcatg cgcgacgagc gggttgtcag cgttcgccgg
     3421 cggctggccg taacgggcga tctcgagacg ggcgcagtgt ccgacccgga actgttcgac
     3481 gctgacctgg aaagcggtgt caggcggttc cagcatcgcc acagcctgtc ggtggacggg
     3541 gccgttggtc ccgctacggt gcgcgccatg aacgtctccg tggaggagcg gatcgaccag
     3601 ctgcgcctgt cccttgaaag ggcgcgatgg atcatgaccg cgcgggacac gtcggtggtc
     3661 gtcgtgaaca tcgcggccgc ggaggtctac gttctggaag aaggtgagat ggtctggcag
     3721 cgtcgggcca tggtcggccg gacctatcgc cagacgccgg tgtttcgcgg ccttatgacc
     3781 tatctggaaa tcaaccccac ctggacggta ccgcccggaa ttctgcgggc ggacatcctg
     3841 ccgaaggtgc gcgaggatcc tggatatctg gcccgctcca acatgacgct gctggaccga
     3901 tcgggtcgcg ccgtcgaccc ctacgccgtg gactggcagg ccctagaggg catgccttac
     3961 atcttccggc aggagccggg cccgacgaac gcgctgggcc tgatcaagtt catgtttccc
     4021 aacgagcact tcgtcttcat ccacgacacc ccgaaccgtg agctcttcga acgggcggag
     4081 cgtacgttca gctcgggctg catcagggtg gaggatccgt tttccctcgc cgccctgctt
     4141 cttcgcgatc cggcgaactg ggacgaagcc gcggtgcgcg ccgcggtgga aaccggtgag
     4201 acccggcggg tccgccttgc ggatccgtgg cccgtctata tcctctactg gaccgcccag
     4261 gtggacgacg acggagtcgt ccgcttctac ccggatgtgt atcagcgcga cgtggagctg
     4321 cttggggaac tcgacggcga cgttcggatc gatcttcccg cctcctgaac ccggctggtt
     4381 cgagcgatgc tgccgactga gggcgctaca tcagtgaaaa aggagaactc gtcacaatgc
     4441 gggcagacag acgcgacttt ctgaagaaaa cgggctctgc ggtcgcggcc gcctccacgg
     4501 cgtccctttg gcccgacctg ctgctcgcag aggaggtgcc caccacggca caagccctgt
     4561 tcgaaacgcg cttcggcgtg agccgtggcg acatgggcag gctcctgtcg ctggcgctcg
     4621 gcaagggggg cgagtttgcc gagctgttct tcgagtacaa gatcgccaac ggggtcacca
     4681 tggaggagga catcgtcaag gagtcctcgg agcgggtcag cctcggtgtc ggcatccggg
     4741 tgctcaaggg agagcagacc ggctacgggt acacgagcga cctcaccttc gagggcatga
     4801 gtcaggcagc gcgcacggcc gcggcgatcg cctctgaagg gggcagggtg gagattgcac
     4861 cgttgaggct ccgcgccaac gccttgcagg tctacgacct cgaacgaccg tttgcagaga
     4921 gttccctgag cagccgaatg atcatcctta ggcagacgta cgatgccgct caggcctacc
     4981 acccgaagat cgataaggtg cgggtgtctc tgagagacga gattcagtac gtcctggttg
     5041 ccaacagcga gggcctgctg gcatcggata cgcggccgca ggtccgactc attgcctcgg
     5101 tcaccgcggc cgacggaggc aatcgcagca cgggactgtc gacggccggt gggcgagtgg
     5161 gatccgcttt tttcagttcg gcgcgcacgc cgaaggagct cggtcgggag gcggccgagg
     5221 aggccatcgt gttgctcggt gcggtcagcc cccgaccggg ggaccagacg gtggtgctcg
     5281 attccggtca atccggcgtt atgattcacg aggcggtcgg ccacccgctg gaagcggact
     5341 cgaaccgcaa aaagcggtcg gtgttctggg acaagctgaa ccagaccgtc gccaatcccc
     5401 tggtcaccat ctacgacgat cccaccattg cgcacttcag gggcagccac aacatggacg
     5461 acgaaggcac gctgtcccga aagaccatgc tgatcgagaa gggcaagctg gtcggctacc
     5521 tgcaggaccg tctctcggca aagctcatga agatgtcgcc gagcggccac ggccgtcgtg
     5581 atggtttcga gaacgtcccg ctgccgcgaa tggccaacac gaacctcgcg cgcggagagt
     5641 ccgacccgga acaaatcgtc cggtccgtca aacgcgggtt cttcgccaag acctaccagg
     5701 gcggcatggt gcagccttcg ggcaagttca ccttctcggt caacatgggt tacctcatcg
     5761 aggacggcaa gctcaccgcg ccggtcaaga acgccacgct gatcggcagc attctggacg
     5821 tgcttcccga ggtgaccatg gtgggcaacg acatgggcac gttcctggga acatgcggca
     5881 agggcggtca gagcgcgccc gtgacggcgg gcacgcccac tctgaagatc aacagcatga
     5941 ccgtgggtgg ccgctcatga tgcgcgacac cagcctgacg gggctcgccg agtcgttggt
     6001 tgcttacgga acccggcgtg
//
LOCUS       DBNHQR010000323         3825 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1550767, whole genome shotgun sequence.
ACCESSION   DBNHQR010000323 DBNHQR010000000
VERSION     DBNHQR010000323.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1550767"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1320)
                     /gene="hisD"
                     /locus_tag="AC16VR_07220"
     CDS             complement(<1..1320)
                     /gene="hisD"
                     /locus_tag="AC16VR_07220"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HIR6010984.1"
                     /translation="MATTYLKRAEPAAAPDEEGIAVRVAEMLAKIRSEGEPAVAGYAR
                     DLDGFDGDFVLSREARDRLIAQLPSETRDDITFAWRQIKRFAEAQRASLHEFEIQTEP
                     GVTLGQRIVPVQVAGCYVPGGRYAHAASALMSVTTAKVAGVPTVVACSPPRGGAIHPA
                     VAFAMDLAGAVVIVQMGGVQAVATLAFGLFGIPPADLLAGPGNAYVAAAKRQLFGEVG
                     IDVIAGPTESAIIADGGADPRLIAVDLASQAEHGPDSPVWLITSSREVAERVMALLPV
                     VAAALPNQDVVMTAWRRHGEVVLCEGREEMVRVSDAYAPEHLQILADDHGWWLEHLSN
                     YGSLFLGEGSTVTHGDKCSGTNHILPTRRAARYSGGLNVQKFLKVLTWQKLDEQANLS
                     FSAVGSRISRVEGMEGHARACDWRLRRYFPGQDWPFEVHGFDELTPGT"
     gene            complement(1320..1598)
                     /locus_tag="AC16VR_07225"
     CDS             complement(1320..1598)
                     /locus_tag="AC16VR_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010985.1"
                     /translation="MANGVIRILALWLIASAVRAAEDFTLELSGGRVMDADITVFDPL
                     TVEDQATYQNPYQPSAGIRHVIVNGVFVVRDEQFVENTFPGRRISSSL"
     gene            1659..2180
                     /gene="thpR"
                     /locus_tag="AC16VR_07230"
     CDS             1659..2180
                     /gene="thpR"
                     /locus_tag="AC16VR_07230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HIR6010986.1"
                     /translation="MRCFVACFLSAESAHRLGTRLEAAWTPPARARLVPRANYHVTLR
                     FFGEVCSAEVSGLLAAVAELGGHDLSCTTGPVTGLPRPARARVIAVGVEPSSPLTDWA
                     EALSGIDPEPEGRAFLPHVTLARIRGTMRVPQIGGLAGVELALHAPSLYETVPVDGGV
                     RYRRVEPERFGSG"
     gene            complement(2172..3191)
                     /gene="mtnA"
                     /locus_tag="AC16VR_07235"
     CDS             complement(2172..3191)
                     /gene="mtnA"
                     /locus_tag="AC16VR_07235"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016493787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0033353 - L-methionine cycle [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HIR6010987.1"
                     /translation="MNDPGFPPSAISWQDGRLRLLDQRRLPEEVAYLEIRTVDAAHEA
                     IRTLAVRGAPAIGIAAAYTLVVAMVADAPTDAAGFRARLAEHARHLTSARPTAVNLAW
                     ACARLLSAAERSADLTTIQAEAEAIHEEDRLICRAIGSHGQPLIPDNAGVLTHCNAGA
                     LAVSELGTATAPMYLSHAAGKPFRVFVDETRPLLQGARLTAWELAAAGMDVTLICDSA
                     AAAFMAAGEIDLAIVGTDRVTANGDVVNKIGTLGVAVLCRHFGIPFYVALPSSTFDPH
                     TATGAQVPIEERDPAEVLGDRAADVPARNPAFDVTPANLVTAFITDRGVLRPPFAESL
                     SSLSA"
     gene            complement(3193..>3825)
                     /locus_tag="AC16VR_07240"
     CDS             complement(3193..>3825)
                     /locus_tag="AC16VR_07240"
                     /inference="COORDINATES: protein motif:HMM:NF012804.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR6010988.1"
                     /translation="RVAEQEGVIRFAYDLQSPDGVVTEESLRETLAAWRTIFRRLGVL
                     GHDPDRYGGLGFGNLSVRDREHPEEFVVTASQTSGRSGLRDRDLVRITGCNLERFWVD
                     AVGHKPPSSETLTHAMIYAADPRIQWVFHVHSPEIWRHAEQLAIPCTAPEVAYGSADM
                     VRAVAELLEKHQSRPLLFATLGHEDGIFACGPTARDAGGLLVSYLAKALA"
BASE COUNT          596 a         1276 c         1342 g          611 t
ORIGIN      
        1 cgtgccgggc gtgagctcgt cgaacccgtg cacctcgaag ggccaatcct gcccgggaaa
       61 atagcggcgc aggcgccagt cgcatgctct ggcgtgtccc tccatcccct ccacgcgcga
      121 tatccgcgag cccacggcgc tgaacgacag gttcgcctgc tcgtcgagct tctgccaggt
      181 cagcaccttc aggaacttct gcacgttgag gccgccggaa tagcgcgcgg cgcggcgggt
      241 cggcaggatg tggttggtgc ccgagcactt gtcgccgtgg gtcaccgtgc tgccctcgcc
      301 aaggaacagg gaaccgtagt tgctgagatg ctccagccac cagccgtggt cgtctgccag
      361 gatctggaga tgctccggcg cgtaggcgtc gctgacgcga accatctcct cgcggccctc
      421 gcacaagacc acctcgccgt gacggcgcca cgcggtcatg accacgtcct ggttcggcaa
      481 ggcggccgcc accacgggca ggagagccat gacacgctct gccacctcgc ggctggaggt
      541 gatcagccaa acgggagagt ccggcccgtg ctcggcttgg gaggcgagat cgacggcgat
      601 caagcgtggg tcggcgcccc catcggcgat gatggcagac tccgtgggac cggcaatcac
      661 gtcgatgccc acctcgccga acagttgccg ctttgcggcg gcaacgtagg cgttgccggg
      721 cccggcgagc aggtcggcgg gagggatccc gaacaggccg aacgccagcg tcgccaccgc
      781 ttggacgccg cccatctgca cgatgacgac ggccccggcg agatccatgg cgaaggcgac
      841 ggcgggatga atcgcgcccc cccgcggggg cgagcaggcg accaccgtcg gcacgcctgc
      901 caccttcgcg gtggttacgc tcatcagcgc agacgctgcg tgggcgtagc gcccaccggg
      961 cacgtagcat ccggcgacct gaaccgggac gatgcgctgg ccgagggtga ccccgggctc
     1021 ggtctggatt tcgaactcgt gcaggctcgc ccgctgggcc tcggcgaagc gcttgatctg
     1081 gcgccaggcg aaggtgatgt cgtcgcgggt ttctgagggc agctgggcga tcagccgatc
     1141 gcgcgcctcc cgggaaagga cgaagtcccc gtcgaacccg tccaggtccc gggcgtatcc
     1201 ggcaacggca ggctccccct cactgcgtat cttcgccagc atctccgcga cgcgtaccgc
     1261 gatgccctcc tcgtccggcg ccgcggccgg ctcggcccgc ttgagatagg tcgtcgccat
     1321 cacaggctgc tgctgatgcg gcggcccggg aacgtgttct ccacgaactg ctcgtcgcgg
     1381 accacgaaca cgccgttgac gatgacgtgt cggatgccag ccgagggctg atacggattc
     1441 tgataggtgg cctggtcctc gacggtgagc gggtcgaaga cggtaatgtc cgcatccatg
     1501 acgcggccgc cgctcagctc caacgtgaag tcctcggcgg cccggaccgc gctggcaatc
     1561 agccagaggg ccagtatgcg gataactccg ttcgccatgc cgcgtctccc gcgcgccgat
     1621 catgaaggcg caagatagaa cggtagaatg gggatgtcat gcgctgtttc gtagcctgct
     1681 ttctgtctgc cgaatccgct caccggctcg ggacgcggct cgaggcggcg tggacaccgc
     1741 ccgcccgggc gcgtctggtc ccgcgagcga actatcacgt cacgctgagg ttcttcgggg
     1801 aggtctgctc tgccgaggta tccgggctgc tggctgcggt ggccgaactc ggcggtcatg
     1861 acctttcgtg cacgacgggg ccggtgacgg gtcttccgcg tccggcgcgt gcccgcgtca
     1921 tcgccgtggg cgtggagccc tcgtcgccac tgacggattg ggcggaagcg ctgtcgggta
     1981 tcgatccgga acccgaaggc cgggcgttcc tgccccacgt caccctggcc cggattcgcg
     2041 ggaccatgag ggtgccgcag atcggtggcc tcgcgggggt ggagctcgcg cttcacgcgc
     2101 cttcgctgta cgagaccgtt cccgtggatg gcggtgtgcg ctaccggcgc gtagagccgg
     2161 agcggtttgg gtcaggctga caagctgctc aacgattccg caaacggcgg tcgcaatacg
     2221 ccgcggtcgg tgatgaacgc ggtcaccaaa ttcgctgggg tcacgtcgaa agcgggattg
     2281 cgggcaggca cgtccgcggc ccgatcgccc agcacctccg ccggatcgcg ttcctcgatg
     2341 ggtacctgcg cgcccgtcgc ggtgtgcgga tcgaacgtgc tcgagggcag cgccacgtag
     2401 aagggaatgc cgaagtgccg gcagagcacg gcgacgccca aggtgccgat cttgttcacc
     2461 acgtcgccgt tggcggtgac gcggtctgtc cccacgatgg ccaggtcgat ctcccccgcg
     2521 gccatgaagg cggcggctgc gctgtcgcag atgagcgtga cgtccatgcc cgcggcggca
     2581 agctcccagg ccgtcagacg cgcgccctga agcagcggcc tggtttcgtc gacgaacacc
     2641 cggaacggct tgccggcggc atggctgaga tacatgggag ccgtggcggt gcccaactcg
     2701 gaaaccgcca gagcaccagc gttgcagtgg gtgagcactc ccgcgttgtc gggaatcaga
     2761 ggctggccgt ggctgccgat ggctcgacag atgagacggt cctcctcgtg gatcgcctcc
     2821 gcctccgcct ggatggttgt gagatcggcc gagcgctcgg cggcgctcag aagccgagcg
     2881 catgcccacg cgaggtttac cgccgtgggg cgtgcggagg tgaggtggcg agcgtgttcg
     2941 gcgagccggg cgcggaagcc ggcggcatcc gtcggcgcgt cggcgaccat ggcgactacc
     3001 aaggtgtagg cggcggcgat gccgatggcg ggggcgcccc tcacggccag ggtgcggatg
     3061 gcttcgtgcg ccgcatccac cgtgcggatc tcgagatagg cgacctcctc gggaagccgt
     3121 cgctgatcga gcagccgcag gcggccgtcc tgccaggaga ttgcgctcgg tggaaaccca
     3181 gggtcgttca tgtcaggcca gcgctttggc caggtagctc accagcagcc cgcccgcgtc
     3241 gcgcgccgtg gggccgcagg caaagatccc gtcctcgtgg cccagggtgg cgaatagcag
     3301 cgggcgtgac tggtgctttt cgagcagctc ggccacggcg cgaaccatgt ccgccgagcc
     3361 gtaggccacc tcaggcgccg tgcaggggat tgcgagctgc tcggcgtgcc gccatatctc
     3421 cgggctgtga acgtggaaga cccactgaat ccgaggatcg gcggcgtaga tcatggcgtg
     3481 ggtgagcgtc tccgacgacg gcggcttgtg ccccaccgcg tctacccaga aacgctccag
     3541 attgcatccg gtgatccgca ccaggtcccg gtccctgagc ccgctgcggc cgctggtctg
     3601 gctggcggtg acgacgaatt cctccggatg ctcccgatcc cggacgctca ggtttccgaa
     3661 acccaaaccg ccgtagcggt ccggatcgtg gccgagaacg cccaggcgac ggaagatggt
     3721 ccgccacgcc gcgagggtct ctctcagcga ctcctcggtt acgacgccat cgggcgactg
     3781 aagatcgtag gcaaagcgga tgacgccttc ttgctcggcc actcg
//
LOCUS       DBNHQR010000324         2017 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1424148, whole genome shotgun sequence.
ACCESSION   DBNHQR010000324 DBNHQR010000000
VERSION     DBNHQR010000324.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1424148"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2017)
                     /locus_tag="AC16VR_07245"
     CDS             complement(<1..>2017)
                     /locus_tag="AC16VR_07245"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6010989.1"
                     /translation="GLLELREYPESNRATITVTTAYPGADADLVQSFITVPLQRAISE
                     AEGIDYLTSDSRQGVSIIEAFMELNYDPNAAIAEIQAKVASQRNLLPQESMDPVIDSR
                     TSLGWALMYLAFTSQEMSPAQVTDYLHRSVIPRLQAIPGVAKAQINGNPTLAMRIWLN
                     PHRMAALGVTATDVRAALQRNNFQAGVGETKGDYVRVTLSSTTGITAREDFARLIIRR
                     DGETLIRLRDVAEVNLDSKNYDQTSWYNGQPAILLALETSPGANPLTVSQAVRVLLPD
                     IESQLPSNMEMQLAHDGSQYIDDSINEVLRTLLEAMLIVLLVIFLSLGSWHAAVVPAV
                     SVPLSLVGACALMLAFGFSLNLLTLLAMLLAIGLVVDDAIVVVENVHRHIAMNKTPFQ
                     AAIDGARELGLPILSMTTTLLAVYAPIAFMGGLVGILFTEFAFSLAGAVLISGLVALT
                     FSPMLSSKVLTSGEEPGRFESAVERFFARLAERYRARLSRALDYQPVTYLFAIVVMGS
                     IYFMFTTSNTELAPTEDREVLNVQITAPETATLEYAVAHVRPAVKMFETFPEQRRTFL
                     MIGGGGTPATSFGGFRFTPVAERERSQMEMQPILQRMLNEIAGVQIAVFSFPSLPGAA
                     RGTPLQFVIASDQDYRQLDTLADDVVQAAYRSGNFLFLNKSVQFALPQ"
BASE COUNT          350 a          595 c          718 g          354 t
ORIGIN      
        1 tctggggcag ggcgaactgc acggatttgt tgagaaacag gaagttgccg ctccgataag
       61 cggcctgcac cacgtcgtcg gccagggtgt ccagctgccg gtagtcctga tccgacgcga
      121 tcacgaactg cagcggcgta cctctggcag cgcccggcag ggaggggaac gagaacacgg
      181 cgatctgcac gccggcgatc tcgttgagca tcctctgcag gatgggctgc atctccatct
      241 gggaccgctc gcgctccgcg acgggcgtga agcgaaaccc gccgaagctg gtggccggcg
      301 tgccgccgcc gccgatcatc aggaaggttc gccgctgctc tggaaacgtc tcgaacatct
      361 tcactgcagg ccggacgtgt gccacggcgt attccagcgt ggcggtttcc ggcgcggtga
      421 tctgaacgtt cagcacctcc cggtcttcgg tgggcgcaag ctcggtgttg ctggtggtga
      481 acatgaagta gatgctgccc atgacgacga tggcgaagag gtacgtgacg ggctggtagt
      541 cgagggctct cgagagcctc gcccgatagc gctcggcgag tcgggcgaag aaacgctcta
      601 cggcgctttc gaatcgcccc ggctcctcgc cgctggtaag caccttggag gagagcatgg
      661 gcgagaacgt caaagccacc agccccgaga tgagcacggc tccggccagg gagaacgcga
      721 actccgtgaa gaggatgccc accagcccgc ccatgaaggc gatgggggcg tagacggcga
      781 gcagggtggt cgtcatggag agaatgggca gtcccagctc ccgggccccg tctatggccg
      841 cctgaaacgg cgtcttgttc atggcgatgt ggcggtgaac gttctccacc accacgatgg
      901 cgtcgtccac caccagcccg atggcgagca gcatggccag cagggtgagc aggttcagcg
      961 agaagccgaa ggccagcatg agcgcgcagg cgccgaccag ggagagcggc acggagacgg
     1021 cgggtaccac ggccgcatgc caggagccga gggaaaggaa gatcaccagc agcacgatga
     1081 gcatggcttc cagcagggtt cgcagcacct cgttgatgga gtcgtcgatg tactggctgc
     1141 cgtcgtgggc gagctgcatc tccatgttcg atggcagctg gctttcgatg tccggcagca
     1201 ggacgcggac ggcctgggaa acggtcagag gattggcgcc tggactggtc tccagggcca
     1261 ggaggatggc cggttggccg ttgtaccagc tggtctggtc gtagttcttc gaatccagat
     1321 tcacctcggc gacgtcgcgc agccggatca gcgtctcgcc gtccctgcgg atgatcagcc
     1381 tggcgaaatc ttccctggcg gtgatgccgg tggtggagct cagggtgacg cgaacgtagt
     1441 cgcccttggt ctcgccgacg ccggcctgga agttgttgcg ttgcagcgcc gcccgcacgt
     1501 cggtcgccgt gacgcccagg gctgccatgc ggtgcggatt cagccagatg cgcatggcaa
     1561 gggtcgggtt tccgttgatc tgtgccttgg cgacgccggg gattgcctgc agccgcggga
     1621 tgacgctgcg gtgcaggtag tcggtcacct gagcggggga catctcctgg ctggtgaagg
     1681 cgaggtacat gagcgcccaa cccaggctcg tgcgcgaatc gatcaccggg tccatggatt
     1741 cctggggcag gaggttgcgc tggctggcca ccttggcctg gatctcggcg atggctgcgt
     1801 tcgggtcgta gttgagctcc atgaaggctt cgatgatcga aaccccctga cgggaatcgg
     1861 atgtcaggta gtcgatcccc tcggcttctg agatggcgcg ctgcaggggc acggtgatga
     1921 agctctgcac caggtccgcg tccgcgccgg ggtaggcggt ggtgaccgtg atcgtggcgc
     1981 ggttgctttc cggatactcc cgcagctcga gcagccc
//
LOCUS       DBNHQR010000325         3319 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_220966, whole genome shotgun sequence.
ACCESSION   DBNHQR010000325 DBNHQR010000000
VERSION     DBNHQR010000325.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_220966"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="AC16VR_07250"
     CDS             <1..687
                     /locus_tag="AC16VR_07250"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HIR6010990.1"
                     /translation="VRRGLLFRCSQLASLSQEALAQFAELGIDVICDLRRPDEREVDP
                     TPVPHHVSRRVEIPMDPGSATQLRDSLRAGYVDVSQRVDFMRTITRELARDHVEGYSS
                     MFAALLEHAPGGFLVHCTAGKDRTGVGVALILLALGVPRETVIHDYMLTNDVMDFEAF
                     ILPRIRQNLGHDNIDVEGARVLSGVREEYIMAALEEMESACGSLEAYLERAIGLTRGD
                     VDALRAHYLS"
     gene            769..2094
                     /locus_tag="AC16VR_07255"
     CDS             769..2094
                     /locus_tag="AC16VR_07255"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HIR6010991.1"
                     /translation="MAGTKHQAPRPTGFDTLALHAGARPDPATGARATPIYQSAAFVF
                     PDSDFAAGLFNIERAGHVYSRLSNPTNAVLEERIAALDGGVGAIATASGQAAMHLAVA
                     TLLGAGDHIVASRSLYGGTHNLLAYTLPRFGISTTFVDPRDPQAFADAVREETRLVFG
                     EILGNPGLEVMNVPAVADAAHAAGLPLMIDATFTTPYLFRPLDHGADLVVHSATKFLS
                     GHGVVIGGLIVDGGTFDWEASARFSTLSQPYDGFHGLVFAEEFGPEAFIMRARKEGLR
                     DFGACMAPMTAFQILQGLETLPLRMDRHVANARAIADFLAGHPMVTAVNHPDLAGHPD
                     HDLAKTLLPRGAGAVMGFDIKGGREAGRAFIESLQLFSHLANVGDAKSLVIHPASTTH
                     HRMDAKALEAAGIGEGLIRVSVGLEDPADLIDDLRGALKRAERAAGVST"
     gene            2091..2897
                     /locus_tag="AC16VR_07260"
     CDS             2091..2897
                     /locus_tag="AC16VR_07260"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6010992.1"
                     /translation="MRVEVGGHCAFAGTGSVPFDARRPCVVFLHGAGFDHSVWVMPSR
                     YFARHGFAVVAPDFPAHGRSKGAPLASVPALASWLAELLDALGVAEAVVVGHSLGSLV
                     GQAFARLFPDRCLGLALLGTSAPMAVTEQLLGASRDDDHAAIDMANTWSHSPQGRLGA
                     SDNPGIWMLGAEERLLERAAPGVYHADFTACNECDLDQLPGLVSCPVLVIAGGSDSMT
                     PARSGQAVANALSARYVSLPGCGHSMLAERPNQVLDALAEFIGQLPPADH"
     gene            complement(2891..>3319)
                     /locus_tag="AC16VR_07265"
     CDS             complement(2891..>3319)
                     /locus_tag="AC16VR_07265"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6010993.1"
                     /translation="AVHLNGTFYCSRAAVALLNRNTDRPEGGAAIINMGSIMGTSGGA
                     GASAYCAAKAGILGLTRSLARELAPYDIRVNAIAPGYIHTDMTSPMEAMYPLLKAQTP
                     QGRIGEPDDIAWAAVYLASLEAKFVTGQTLSPNGGWHMSQ"
BASE COUNT          495 a         1097 c         1134 g          593 t
ORIGIN      
        1 gttcgtcgtg ggttgctgtt ccgttgcagt cagcttgcca gcctgagcca ggaagcgctg
       61 gcgcagttcg cggagctggg catcgacgtg atctgcgatc tcaggcggcc cgacgagcgg
      121 gaggtggatc cgacgcccgt gccgcatcac gtctcccgcc gggtcgagat tcccatggac
      181 ccgggcagcg cgacccagct gcgggactcg ctgcgggctg gttacgtgga cgtgagccaa
      241 cgggtcgact tcatgcgcac catcacgcgg gagctcgccc gcgatcacgt cgagggttac
      301 tcgtcgatgt ttgccgcgct gctcgagcat gccccgggcg ggttcctcgt gcactgcacc
      361 gccggaaagg accgcacggg ggtcggggtg gcgctgatcc tgctggcgct cggcgtgccc
      421 cgggaaaccg tcatccacga ctacatgctc accaacgatg tgatggattt cgaggcgttc
      481 atactgccgc gcatccgcca gaatctgggt cacgacaaca tcgacgtcga aggggcccgg
      541 gtgctgtcgg gggttcgcga agagtacatc atggccgctc tggaggagat ggagagcgcc
      601 tgcggaagcc tggaagccta cctggagcgc gccatcgggc tcacccgcgg tgatgtcgac
      661 gcgctgcgcg cgcactacct gagctagggt ccgggcccgg agaacggatt gccactgccc
      721 gtggggctcg gttagcctcg tcgccctcct atgcggaagg cgcccatcat ggcaggcaca
      781 aaacaccagg ctccccgacc taccggattc gatactctgg cgctgcatgc cggcgcgcga
      841 ccggatcctg caacgggcgc ccgggcgacg cccatctatc agagcgccgc tttcgtcttc
      901 cccgactcgg acttcgccgc cgggcttttc aacatcgagc gcgccgggca cgtttactcg
      961 aggctttcga atcccaccaa tgccgtgctg gaggagcgca tcgctgcgct ggacggtggc
     1021 gtaggcgcca tcgccacggc cagcggccag gccgccatgc atcttgcggt ggccacgctg
     1081 ctgggcgcag gagatcacat cgttgcttcg cgatctctct atggcggcac ccacaatctt
     1141 ctggcgtata ccctgccgcg attcggtatc agcaccacct tcgtggatcc tcgcgacccc
     1201 caggcgtttg ctgacgccgt gcgggaagag acccgcctgg tgttcggtga gatcctcggc
     1261 aacccggggc tcgaggtgat gaacgtcccc gccgttgccg acgcggcgca cgcagcgggg
     1321 ctcccgctga tgatcgacgc cacgttcacc accccgtacc tgttccgccc tctcgatcac
     1381 ggcgcggacc tggttgtgca ttccgcgacc aagttcttaa gcggccacgg agtggttatc
     1441 ggcggcctga tcgtcgacgg cggaaccttc gactgggagg cctcggcgag gttttcaacc
     1501 ctgtcccaac cctacgacgg tttccacggg ctggtatttg ccgaagagtt cggcccggaa
     1561 gcgttcatca tgcgcgcccg gaaggagggt ctgagagatt tcggtgcgtg catggcgccg
     1621 atgacggcct ttcagatcct ccagggcctc gaaacgctgc cgctgcgcat ggaccggcac
     1681 gtggccaatg ctcgagcaat tgcggatttt ctagccgggc atcccatggt gaccgccgtg
     1741 aaccatcccg atcttgccgg gcaccccgat cacgacctgg cgaagacgct gctgccgcgc
     1801 ggagcgggtg cggtgatggg attcgacatc aagggcgggc gggaagccgg gcgcgcgttc
     1861 atcgaaagcc tgcagctctt ctcgcacctg gcgaacgttg gggacgcgaa gtcgctggtc
     1921 atccatccgg ccagcacgac ccaccatcgc atggacgcaa aggccctcga ggcggccggg
     1981 atcggcgaag ggctcattcg ggtatcggtc gggctcgaag atccggccga cctgatcgac
     2041 gatctgcggg gggcgttgaa gcgggccgag cgggcggccg gagtgtccac atgagggtgg
     2101 aagtcggcgg tcactgcgcc ttcgcgggca cgggcagcgt gccgttcgac gcacggcggc
     2161 cctgcgtggt gtttcttcac ggggcgggct tcgatcacag cgtctgggtc atgcccagcc
     2221 gttacttcgc gcgacacggt ttcgcggtgg tggcgccgga ctttcccgcc cacgggcgat
     2281 cgaagggggc gccgttggct tctgtaccag cccttgcatc ctggcttgcc gagctgctcg
     2341 acgcgctggg cgtggcggag gccgtcgtcg tcggtcacag cctgggctcc ctggttgggc
     2401 aggcctttgc ccggctcttt cccgaccgct gcctggggct cgcgctgctg ggcacgtccg
     2461 cgcccatggc ggttaccgag cagctgctgg gagcgtcccg ggacgacgat cacgcagcca
     2521 tcgacatggc gaatacctgg agtcacagtc ctcagggtcg gctcggggcc agcgacaacc
     2581 caggcatctg gatgctgggc gccgaggagc gtctgctgga gcgcgcggct cccggcgtct
     2641 atcacgccga cttcaccgcc tgcaacgagt gcgatctgga tcagctgccc gggcttgtga
     2701 gctgccccgt gctggtgatc gccggaggtt cagacagcat gacgcccgcg cgctcggggc
     2761 aggcggtggc caatgccctt tcagcgcgct acgtaagcct tcctggctgc ggtcactcca
     2821 tgctcgccga gcggcccaac caggtgctcg acgccctggc ggagttcatc ggccagctgc
     2881 cccccgccga tcactgactc atgtgccacc cgccgttcgg cgacagggtc tgaccggtga
     2941 cgaacttcgc ctcgaggctt gccaggtaga cggcggccca ggcgatgtcg tctggctcgc
     3001 cgatgcggcc ctggggcgtc tgagccttga gcagcgggta catggcttcc attgggctcg
     3061 tcatgtccgt gtgtatgtat cccggcgcga tggcgttcac cctgatgtcg taaggcgcaa
     3121 gctcccgggc cagggaccgg gtgagcccga ggatcccggc cttggcggcg cagtaggcgg
     3181 aagccccggc gccacccgag gtgcccatga tgctgcccat gttgatgatc gcggcgccgc
     3241 cctccggccg gtctgtgttg cggttgagca acgccacggc cgcgcggctg cagtagaacg
     3301 tgccgttgag gtggacggc
//
LOCUS       DBNHQR010000326         2365 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_31809, whole genome shotgun sequence.
ACCESSION   DBNHQR010000326 DBNHQR010000000
VERSION     DBNHQR010000326.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_31809"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="AC16VR_07270"
     CDS             <1..830
                     /locus_tag="AC16VR_07270"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6010994.1"
                     /translation="IAVVTAEHFFLFDVGARSPVRIAQARLPMARINGVFLTHFHSDH
                     IAALPDVNLASWVQSRPAALTVYGPPGVGAVVDGFNTAYRLDRGYRTAHHGAELLPPE
                     RGPMLAKTFVPGDVVWRDDLMTVTSFEVEHPPIEPAVGYRVDYRGRSVVISGDTNAAD
                     TLFTVAKDADLLLHDALSRTLLDPMIATATELGVPVLPTIMTDVIDYHADVTSLPARA
                     AEAGIRQLALYHMVPVPPNALAAKMFLRGIPDDVIVTRDLHTFDLPANSTDIVISEP"
     gene            834..2120
                     /locus_tag="AC16VR_07275"
     CDS             834..2120
                     /locus_tag="AC16VR_07275"
                     /inference="COORDINATES: protein motif:HMM:NF013488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR6010995.1"
                     /translation="MKLKTRLRSAAMRQLSSAAYLEKQRAKARKLRQRKGEPPVVHYF
                     HQVDDPYSHLAVQKLDELDDRYDLAFQPHLASKPGEDYRGSAAHFDHWARKDAASVAA
                     DYGTRFAPAIDAPAGDAVDVANRVLSAHLDRDDFARAAVSIGEALWSDQRIAEPADAH
                     DGLRAVAEGNDLRRALGHYQGAMFYFEGEWFWGVDRIRLLERRLIDEGFGSSETLCVP
                     EPTPTETSGLDTSSVLLEYFPSLRSPYTAIGHARVLDLIARSGVCVRVRPVMPMMMRG
                     IPAPRAKQRYIITDAGREGRAHGAPFGPIVDPFGDPVKRAFALLPGAAAAGREMEYVT
                     AYLNAAWFEGVDITTQKGLRAVATNAGMDWAALEQASRQENWEAILEENLNTMLKEDL
                     WGVPSFRISGGNSDEAFACWGQDRIWRVENEIASRT"
     gene            2181..>2365
                     /locus_tag="AC16VR_07280"
     CDS             2181..>2365
                     /locus_tag="AC16VR_07280"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR6010996.1"
                     /translation="MRAAEKLFAERGIENVSIRDIVGEAGQKNESALQYHFKSLTGLI
                     SAIHAERSAETQARRGEL"
BASE COUNT          444 a          784 c          763 g          374 t
ORIGIN      
        1 gcatcgcggt ggtcacggcc gagcactttt tcctgttcga cgtcggcgcg cgctcacctg
       61 taagaatcgc ccaggcccgg ctgcccatgg cgcgcatcaa cggtgtcttc ctcacccact
      121 tccactcgga tcacatcgcc gcgctgcccg acgtgaacct ggcctcctgg gtgcagagca
      181 gaccggccgc gctgacggtg tacggcccgc cgggagtcgg ggcggtggtg gacggattca
      241 ataccgctta ccggctagac cgcggctacc gcacggctca ccacggcgcc gagctgttgc
      301 cgcccgaacg cggcccgatg ctggcgaaaa cgttcgtccc cggcgacgtc gtctggcgcg
      361 acgacctgat gacggtgacc tcgttcgaag tggagcatcc gcccatcgag cccgcggtgg
      421 gctaccgcgt cgactatcgg ggacgctcgg tggtgatctc cggagatacc aatgcggcgg
      481 acaccctgtt caccgttgcc aaagacgcag acctgctgct gcacgacgcg ctgtcgcgta
      541 cgctgctgga cccgatgatt gccaccgcca ccgagttggg cgttccggtc cttccgacca
      601 tcatgaccga cgtcatcgac tatcacgccg acgtcaccag cctgcctgcg cgcgcggcgg
      661 aagccggcat caggcagctg gccctgtacc acatggtgcc cgtcccaccc aacgcgctgg
      721 cggcaaagat gttcctcagg ggcattccgg acgacgtcat cgtcacgcgg gatctgcata
      781 ccttcgattt gcccgcgaac tccacggaca tcgtcattag cgagccttga gccatgaaac
      841 tcaaaacccg actgcgctcg gctgccatgc gccagctctc ctcggccgcc tatctggaga
      901 aacagagggc aaaggcgcga aagctgcggc agaggaaggg cgagccgccg gtcgtgcact
      961 acttccacca ggtggacgat ccctacagtc acctggcggt gcagaagctc gacgagctag
     1021 acgaccgcta cgatctcgct tttcaacccc acctggcttc caagccaggc gaagactacc
     1081 ggggcagcgc cgctcacttc gatcactggg cacgcaagga cgccgcaagc gtggccgcgg
     1141 attacggcac ccggttcgcg cccgccatcg acgcgcccgc cggcgatgcc gtggatgtgg
     1201 caaaccgggt gctgtcagct cacctggatc gcgacgattt cgcccgcgct gccgtcagca
     1261 ttggcgaggc gctgtggagc gatcagagaa tcgccgagcc ggccgacgct cacgacggcc
     1321 tccgggccgt tgccgaagga aacgacctgc gccgcgcgct cgggcattac cagggagcca
     1381 tgttctactt cgaaggggaa tggttctggg gtgtcgaccg aattcgtctg ctggagcgaa
     1441 ggctgatcga cgagggcttc ggcagcagcg agaccctttg cgtgcccgag ccgaccccaa
     1501 cggaaaccag cggcctcgac accagttctg tgctgctgga gtacttcccg tctctgcgca
     1561 gcccctacac ggccatcggt cacgcccgcg tgctcgacct catcgcacgc agcggggttt
     1621 gcgttcgagt gcggccggtg atgccgatga tgatgcgggg cattcccgcg ccgcgcgcga
     1681 agcagcgcta catcatcacg gacgccggcc gcgaagggag agcacatggc gcgccgttcg
     1741 gacccatcgt cgatccgttc ggggatccgg tgaagcgtgc ctttgctctg ctccccggtg
     1801 cggccgcagc cggccgggag atggagtacg tcaccgcata cctcaacgcc gcctggttcg
     1861 agggcgtgga catcacaacg cagaaaggcc tgagagcggt ggcgacaaac gcgggaatgg
     1921 attgggccgc gctcgagcag gccagccggc aggagaactg ggaagccata ctggaggaaa
     1981 acctcaacac gatgctcaaa gaagaccttt ggggcgtgcc gagttttcga atctcgggcg
     2041 gaaacagtga cgaggccttc gcgtgctggg gacaggaccg catctggcgg gtcgagaacg
     2101 aaatcgcgag ccgaacgtag atgccggcgc ggagccaagc gggtagacac tcgagaagca
     2161 gggccaccca ggacgcgctg atgcgcgccg cggaaaaact gttcgcggaa cgaggcatcg
     2221 agaacgtgtc gattcgcgac atcgtgggcg aagcgggtca gaagaacgag tccgcgctgc
     2281 agtaccattt caagagcctc accggcctca tctctgccat tcacgccgag cgatcggcgg
     2341 aaacccaggc ccggcgcgga gagct
//
LOCUS       DBNHQR010000327         3888 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_601692, whole genome shotgun sequence.
ACCESSION   DBNHQR010000327 DBNHQR010000000
VERSION     DBNHQR010000327.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_601692"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="AC16VR_07285"
     CDS             complement(<1..893)
                     /locus_tag="AC16VR_07285"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017809.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transcriptional activator/DNA
                     repair enzyme AdaA"
                     /protein_id="HIR6010997.1"
                     /translation="MLPAPEICERARLARDPRFDGRFLTGVVTTGIYCRPICPARPAG
                     RDNVRYFPTAAAAEAQGYRACRRCRPEVAPGVPEWAISSDRVIRGLRLIDGGFLADQS
                     TAALAARLGVSTRHLNRLFHQELGATPSSLARSRRLHMALRLIVETDLGFCDVALQAG
                     YGSVRRFNAEVSGTFGRSPRALRGVRDEELTRELTLKLPVREPFDAPWMFRFLEQRAL
                     PGLEEVHGLEYRRRVGSGESWITVSQRGGALMLKVPAGTSASLSSILRRVRRLFDLQA
                     DSAVIDAHLARDPGLAPLVAAS"
     gene            963..2180
                     /locus_tag="AC16VR_07290"
     CDS             963..2180
                     /locus_tag="AC16VR_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6010998.1"
                     /translation="MSKAPEQPAIDYPDSDAGLDTALAAWLQDPGGADAYRQDISFRP
                     PGGAHAAPGAVEQIAAQYRFARGEHGVSVTASLDGRSLSLDAPVRLEPVSIPKPWGRE
                     LWYTGMEVRGESRVVAGEASLALSRYLALAPARLCRRRPIVLLKILDPAATPVAGDLY
                     FEVHEHKREVYVVTHVDPTAWPDGTGAIRLGMSQSRRAAFAEDDAFRNAYLAAVKAYE
                     TVRRRIDGGERLPPETETQLREAMDGFTAFAPLRVGDVVTVHPWQPHSLQHGVRVVEF
                     QTPTYERYILSFGQRVLTQNHWDTEAVLDRIDLGPPRPESLGPVAAGIGQIADFDDFS
                     AFRVEIPPGVSRPLEIELPYAVCMGVEGRVSVNGLALDAEEACFVPASALPVTLANAA
                     ATPAVALVAAAKL"
     gene            2184..3263
                     /locus_tag="AC16VR_07295"
     CDS             2184..3263
                     /locus_tag="AC16VR_07295"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR6010999.1"
                     /translation="MEATRKIRVRLWRRIAPIVVVSAFYYGVLVYPVLRILGLAWPDW
                     QPGTWQLLAIMVGPLAGRLAYEAIPCSATRWLSALALTWLGICFVAFTLVLGWEVANL
                     LLPLPGRESGWALLGLTGFACLVGYVNAQRVAVRNIDVPAPQIGKRLRVAQISDVHVG
                     SRSGRFLRRVVKRTNAQDPDLVFITGDLVDFRDISQQELGSLSDLEAPAYFVIGNHER
                     YVDCEAICDRLRNLGVHVIRNDTVTIDRIQLVGIDDAEPKTQVGKILQTLEPDPHRYR
                     ILLYHRPDGARDAAAWGVNLMLCGHTHAGQIVPFNLLVKRVFPRIRGVYRVDGLTLYV
                     SPGTGTWGPILRLGSRSEISMMTLG"
     gene            3286..>3888
                     /locus_tag="AC16VR_07300"
     CDS             3286..>3888
                     /locus_tag="AC16VR_07300"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6011000.1"
                     /translation="MAEEQELIVEQIEIGPMQNFTYIVGSRSAREVAIVDPAWDVEHL
                     VRHIEEKGYKLTAALVTHYHPDHIGGSFGGHNIDGIAELLAINPLKVYAHKEEAEGVR
                     KVTGISESDLVRVESGDDLRIGDVSIEFLHTPGHTPGSQCFRVHNTLVSGDTLFIQGC
                     GRVDLPGSNSEDMYHSLQKLKSLPDETLLLPGHTYAHVPNA"
BASE COUNT          664 a         1329 c         1253 g          642 t
ORIGIN      
        1 gacgcggcaa cgaggggcgc aaggcccgga tcccgcgcga ggtgcgcgtc gatgacggca
       61 gagtccgcct ggaggtcgaa cagccgcctg acgcgccgaa gtatcgagga cagcgacgcc
      121 gaagtgcccg cgggcacttt cagcatcaac gcgcccccgc gctgggatac cgtaatccag
      181 ctttccccgg atccgacccg ccggcgatac tcgagcccgt gcacctcctc gagaccgggc
      241 aacgcgcgtt gctcgagaaa gcggaacatc cacggcgcat cgaaaggctc acggaccggc
      301 agcttaaggg tcagctcccg ggtcagttct tcgtcacgca ccccgcgcag agcccgaggc
      361 gaacgtccga acgtcccgct cacctcggcg ttgaagcgcc ggacgctgcc gtagcccgcc
      421 tgcagggcaa catcgcagaa gcccaggtcg gtctcgacga tgagccggag agccatgtgc
      481 agccgccggg acctcgccag gctgctgggc gtggccccga gctcctggtg aaagagcctg
      541 ttgaggtgcc gggtgctcac tccgagcctg gctgcgagcg cggccgtgct ctgatcggcc
      601 aggaatcccc cgtcaatcag ccgcagcccc ctgatcaccc tgtcgctgga gatcgcccac
      661 tccggcaccc ccggcgcgac ctccggccga cagcggcgac atgcccgata cccttgggct
      721 tccgccgccg cagccgtagg aaagtagcgc acgttgtccc ggccggcggg ccgggccggg
      781 cagatgggcc tgcaatagat cccggtggtg acgacgccgg tgaggaaccg gccgtcgaag
      841 cgggggtctc tggccaggcg ggcgcgctcg cagatctctg gggcaggtaa catgctggcc
      901 tcttcagcag cttccccgac tataatcccg gcctcgcgaa aaaactcgcc ggaatcggac
      961 atttgtccaa agcgcccgaa cagcccgcca ttgactatcc cgacagcgac gccgggctgg
     1021 acacggccct ggccgcttgg ctgcaggatc ccggtggggc ggacgcctat cgtcaggaca
     1081 tttccttccg accgcctggg ggcgctcacg ctgctccggg cgctgtcgag cagattgccg
     1141 cgcaataccg attcgcccga ggcgagcacg gcgtgagcgt gaccgcgtcg ctggatggac
     1201 gaagcctgtc cctcgatgcc cccgtccgcc tggagccggt cagcataccg aaaccctggg
     1261 gccgggaact gtggtacacg ggtatggagg tacgcggcga aagtcgcgtg gttgccggcg
     1321 aggccagcct ggccctgtcg cgctatcttg cgctcgcacc ggcgcgcctg tgccggcgac
     1381 gtccgatcgt ccttttgaaa attctggatc ctgccgccac gcccgtggcg ggcgacctct
     1441 atttcgaggt gcacgagcac aagcgcgagg tgtacgtcgt cacccacgtg gacccgaccg
     1501 catggcctga cggcacgggg gcgatccgac tcgggatgag ccagtcgcga cgcgcagctt
     1561 ttgccgagga cgacgcgttc cgaaacgcct acctcgcggc ggtgaaggcc tacgagaccg
     1621 ttcgccgccg cattgacggg ggcgaacggt tgccgccgga aaccgagacg cagctgcgcg
     1681 aagccatgga cggcttcacg gcctttgcgc cgctgcgggt cggtgacgtg gtgaccgtcc
     1741 acccctggca gccgcattcg ctgcagcatg gtgtgcgcgt cgtcgagttc cagaccccaa
     1801 cctacgagcg ctacattctg tcgttcggcc aacgggtgct cacccagaac cactgggaca
     1861 ccgaggccgt cctcgaccgg atcgatcttg gccctccccg gccggagagt ttggggccgg
     1921 tcgccgccgg catcgggcag atcgcggact tcgacgattt ctccgccttt cgggtggaga
     1981 tccctcccgg ggtctcccgc ccgctggaaa tcgagctgcc ttacgccgtg tgcatggggg
     2041 tagaaggccg cgtatcggtg aacgggctcg ccctggatgc agaagaggcc tgcttcgtgc
     2101 ccgcctcggc actccccgtg accctggcaa acgctgccgc caccccggcc gttgctctgg
     2161 tggcggcggc caagctctga gccatggagg cgacgcgcaa gatccgggtc aggctgtggc
     2221 ggcgcatcgc acccatcgtc gtcgtttcgg ccttctacta cggcgtcctg gtctatcccg
     2281 ttctgcgcat tctcggcctg gcatggcccg actggcagcc gggcacatgg cagctgctcg
     2341 caatcatggt gggtcccctc gcgggacgcc tggcctacga ggccataccc tgcagcgcga
     2401 cccgctggct gtcggcgctg gcgctgacct ggctggggat ctgcttcgtc gcgttcaccc
     2461 tggtgctcgg ctgggaggtg gccaacctgc tgctgccgct gccgggtcgc gaatccggct
     2521 gggcgcttct gggactgacc gggtttgcct gcctggtggg ctacgtcaac gcgcagcgcg
     2581 tggcggtcag gaacatcgac gttcccgcac cacagatcgg caagcgcctg cgcgtagcgc
     2641 aaatcagcga cgtgcacgtc ggctcccgca gcggccggtt tctccgccgg gtggtgaaac
     2701 gcacgaacgc ccaggatccg gacctggttt tcatcaccgg cgacttggtg gacttcaggg
     2761 acatctccca gcaggagctg ggctccctga gcgacctcga agcgcccgcc tacttcgtca
     2821 tcggcaacca tgagcgctac gtcgactgcg aggccatctg cgatcgcctg aggaatctcg
     2881 gggtgcacgt gatacgcaac gacaccgtga cgatcgacag gattcagctc gtcggcatcg
     2941 acgatgccga gccgaagacc caggtcggca agatcctgca gacgctcgag cccgaccctc
     3001 acaggtaccg gatactgctg tatcaccgtc cggacggggc gcgggacgcg gcggcctggg
     3061 gcgtcaacct catgctctgc gggcacaccc acgcggggca gatcgtgccg ttcaatctgc
     3121 tcgtcaaacg agtattccca cgcatccggg gcgtttaccg ggtggatggt ttgaccctgt
     3181 acgtctctcc gggcaccgga acgtggggcc ccattctccg cttaggatct cgttcagaga
     3241 tcagtatgat gacccttggc tgaagaaacg accggacaag caatcatggc agaagagcag
     3301 gaactcatcg tcgagcagat cgagatcggg cccatgcaga acttcacgta catcgtgggg
     3361 tctcgatcgg cgcgcgaggt ggccatcgtg gacccggcat gggacgtgga gcacctcgtg
     3421 cgccacatcg aggaaaaggg ctacaagctc accgcagcgc tggtgactca ctaccatccc
     3481 gatcacatcg gcgggtcctt tggcgggcac aacatcgacg gcatcgccga gctgctcgcc
     3541 atcaatcctc tcaaggtcta cgcgcacaag gaggaagccg aaggagttcg caaggtcacg
     3601 ggcatatccg agtccgatct cgttcgcgtg gagtccggcg acgatctgcg catcggagac
     3661 gtgagcatcg agttcctgca cacgcccggg cacacgcccg gctcccagtg ctttcgagtt
     3721 cacaacacgc tggtgtccgg cgacaccctc ttcattcagg gctgcgggcg cgtggacctt
     3781 cccggatcga actccgagga catgtaccac agccttcaga aactcaaatc gctccccgac
     3841 gagacccttc ttctgccggg acacacctac gcccacgtcc ccaacgca
//
LOCUS       DBNHQR010000328         3825 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_601698, whole genome shotgun sequence.
ACCESSION   DBNHQR010000328 DBNHQR010000000
VERSION     DBNHQR010000328.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_601698"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="AC16VR_07305"
     CDS             complement(<1..274)
                     /locus_tag="AC16VR_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011001.1"
                     /translation="MGSLLVVIAIACTTVLWVKGARKNRQRWLARLDLPGTWRWRPSD
                     SAESEVQGSLELSGGLSSGAYRLVEADVEEAGNWALEGHTLLLSPTG"
     gene            complement(278..1303)
                     /locus_tag="AC16VR_07310"
     CDS             complement(278..1303)
                     /locus_tag="AC16VR_07310"
                     /inference="COORDINATES: protein motif:HMM:NF022723.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3080 family protein"
                     /protein_id="HIR6011002.1"
                     /translation="MWTSAVAGWCRRFALAGGVLVGVQCSGPPDNAFADYLSRVQRLL
                     ETDVSVDAEGSLPRYPNRGLLRRDVASLSIDVLDFIELHDCDLAGVVGFRNSPLGRLQ
                     TPSQRLGYESEWLAGARSCASDGVGWLEPLIAKKEAALPAVFWNATFAGEEFARLLGR
                     FEASETPGAGYLLRELGDAYATLATDRFDLGGFERVLGALGSIPWLGQARRAWDLERR
                     HLDAVAGALTEGLEGVCRNGAPTPKSRALVNVFQRYYVDALQPGIARRHGTDRDAVAA
                     VAALVGLFGGDVPGEFAVWYGQVLAPEPRRSEWSRLASVRLEHAAAWNRLFARCGVDP
                     ARGLAQH"
     gene            complement(1272..1556)
                     /locus_tag="AC16VR_07315"
     CDS             complement(1272..1556)
                     /locus_tag="AC16VR_07315"
                     /inference="COORDINATES: protein motif:HMM:NF016351.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HIR6011003.1"
                     /translation="MNVAMLQVVFHLSGCRSLKEKRQRLKGIRDRFGRSSNVAVCESA
                     YQDSLQRAEWSFVASASSRAVVEKSLAEIERSLSLSVDAELVDVRRRWLV"
     gene            complement(1553..2077)
                     /locus_tag="AC16VR_07320"
     CDS             complement(1553..2077)
                     /locus_tag="AC16VR_07320"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6011004.1"
                     /translation="MPTLTTERLLLRPLDDSDISDLYEFWNDPEVRRYLWEDQIMSRD
                     AVAQIVADSVRRFQALGSGFFAIEMAANPGSLVGFCGHREFEDGEQVELLFGIVPEFW
                     GEGFVTEAAMEVLRHGFETCGIVRVIAATETPNQRSVRVLQRLGMVFEERRKHHGLDT
                     VFYSLTSEGFASSG"
     gene            2156..2947
                     /locus_tag="AC16VR_07325"
     CDS             2156..2947
                     /locus_tag="AC16VR_07325"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6011005.1"
                     /translation="MSEIAPAVPSATILMLRDGPRGLEVFMVVRHHEIDFASGALVFP
                     GGKADPGDFDEGLLPRLDGADADPTLRGIQVAAIREAFEECGILLARATGEDGLISGG
                     RLAELERYRNALHAGELSMLEFAEREDIRLACNELTHFAHWITPENLPKRFDTHFYLA
                     MAPADHLALHDGHESVDSVWITPAQAIADAESGTRTVIFPTLRNLAKLGFSDTAEHAV
                     AAAATDTVVPVLPSIEQRDDGTYICIPPEAGYDISEERVPQPRSG"
     gene            complement(3003..3776)
                     /locus_tag="AC16VR_07330"
     CDS             complement(3003..3776)
                     /locus_tag="AC16VR_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011006.1"
                     /translation="MKRIILALSLMTAPLLAGAEEAAPTPMYVAATNLTVSGHPGAQN
                     RFETFIKRAAEAHAKLDTKRHWFAWQQEMGNPRSYTLARTFGSMTAMDETPMNPIAEA
                     FGPEEIAKLTPDHEVVASVERGVWVQRPDLAVMPDSPVGQRVIWLQATVRAGENERFE
                     AYVAKVAEATREVAPEVRYATFAPVFGSQNTYVFSVPTSYAQLDEAPVMSVSERLRGA
                     FGEREARRLEAERAATVESLVQLLVRPRPDLAYQPPAEE"
BASE COUNT          687 a         1362 c         1167 g          609 t
ORIGIN      
        1 ccccggtcgg gctcaaaagc agggtatgtc cctctaaagc ccagttcccg gcctcttcga
       61 catcggcttc caccagccga taagctccag agctcaggcc accgctcagc tcgaggctgc
      121 cctgaacctc cgattcggcg gaatcagacg gccgccagcg ccacgtaccg ggcagatcca
      181 ggcgcgcaag ccagcgctgc cggttcttcc gggcaccttt cacccagagc acggtagtgc
      241 aggcgatggc aatgacgacc agcagagaac ccatggctca atgctgcgcc aagccccgcg
      301 ccggatcaac tccacagcgc gcaaagagcc tgttccaggc agcggcgtgc tcaaggcgca
      361 ccgacgcgag tcgggaccat tcactccgcc gaggctcggg ggcgagcacc tgtccgtacc
      421 agacggcgaa ttcgccgggc acgtcgccac cgaagagccc gacgagcgcg gcaactgcag
      481 caacggcatc ccggtcggtc ccatgccgcc gcgcgatgcc cggctgcagg gcgtcgacgt
      541 agtaacgctg gaagacgttt acgagtgccc gactcttcgg cgtcggggcg ccgtttcgac
      601 acaccccttc gagcccctcg gtcagcgccc cggccaccgc gtccagatga cgcctctcca
      661 gatcccacgc ccgtctggcc tggccaagcc aggggattga gccgagcgct ccgagcaccc
      721 gctcgaaccc gccgaggtcg aaccggtcgg tggcgagcgt cgcgtaggcg tcccccagtt
      781 cccgcagcag gtagcccgcc cccggcgtct cggacgcctc gaacctaccc agcagccggg
      841 cgaactcctc ccccgcaaac gtggcgttcc agaagacggc cggaagcgca gcctccttct
      901 tcgcgatcag cggctccagc caccccaccc catcggacgc gcaggaccga gcgcccgcaa
      961 gccactcaga ctcgtagccc aggcgctggc tcggcgtctg caaacgcccc agcgggctgt
     1021 tgcgaaaccc caccaccccg gccaggtcgc agtcgtgcag ctcgataaag tccagaacgt
     1081 cgatgctcag tgacgccacg tcgcggcgca ggaggccccg attcggataa cgaggcaagg
     1141 agccttcagc gtccacggaa acgtcggttt cgagcaggcg ctgaacgcga gacaggtagt
     1201 cggcaaaggc gttatccggc gggccgctgc actgaacgcc gaccagcaca cccccggcca
     1261 gcgcgaagcg cctacaccag ccagcgacgg cggacgtcca caagctctgc atcgacgctg
     1321 agactcaggc tgcgttcgat ctcggcaagg ctcttctcga ccacggctcg gctgctcgcc
     1381 gacgccacga acgaccactc tgctcgctgc agggagtcct ggtaagcgct ttcgcatacg
     1441 gccacgttcg agcttcgccc gaaccggtcc cggatcccct tgagacgctg acgcttttcc
     1501 ttgagggagc gacacccgct gagatggaag accacctgaa gcatcgcgac gttcacccgg
     1561 aagacgcgaa cccttcgctg gtcagtgagt agaagaccgt gtccagacca tggtgcttac
     1621 gtcgttcctc gaaaaccatg ccgagccttt gaagaacccg gacggatcgc tgattcggcg
     1681 tctccgtggc ggcgatcacc ctaacgatgc cgcaggtttc gaagccgtgc cgcagcacct
     1741 ccatggccgc ctcggtcacg aatccctctc cccagaactc cggcacgatg ccgaacagca
     1801 gctccacctg ttcgccgtct tcgaactccc ggtgaccgca gaatccaacc agcgagcccg
     1861 gattggcggc catttcgatg gcgaaaaagc cggagccgag cgcctggaac cggcgcacgc
     1921 tgtccgccac gatctgagcg accgcgtcgc gcgacatgat ctggtcctcc cagaggtagc
     1981 ggcgcacttc cgggtcgttc cagaactcgt acagatcgga gatatccgag tcgtccagcg
     2041 gcctgagcaa cagccgctcg gtcgtcagcg tcggcatgtg aaacttccct cccgcgcccg
     2101 agtttgccat agcccgggac ataagcgaac atcccggccc ttcgagggag acacgatgag
     2161 cgagatcgcc ccggccgtgc cgtcggccac catcctcatg ctgcgcgacg gaccgcgggg
     2221 gctggaggtc ttcatggtgg ttcgccacca cgagatcgac ttcgcctcgg gcgcgctcgt
     2281 tttccctggc gggaaggcgg atcccggcga tttcgacgag ggcctgctgc cccggctgga
     2341 cggcgcggat gcggacccta ccctgcgcgg cattcaggtc gcggccatcc gagaggcctt
     2401 cgaggagtgc ggcatcctgc tcgccagggc gacgggcgaa gacgggctga taagcggcgg
     2461 ccggctcgcc gagctggagc ggtaccgaaa cgccctgcac gccggcgagc tgtcgatgct
     2521 ggagtttgca gagcgggaag acatccgtct cgcctgcaac gagctgaccc actttgctca
     2581 ctggatcacc ccggagaacc tgccaaagcg attcgacacc cacttttatc tggccatggc
     2641 tcccgccgat catctggccc tgcacgacgg tcacgagtcc gtggattcgg tctggatcac
     2701 gcccgcccag gcgatcgccg atgcggaaag cggcacccgg accgtcatct tcccgacgct
     2761 tcgcaacctc gccaagctgg gattcagcga caccgccgag catgcggttg ctgcggccgc
     2821 aacggacacc gtagtgccgg ttctcccgag catcgagcag cgcgacgacg ggacctatat
     2881 ctgcatcccg cccgaggcag gctacgacat ctccgaagag cgcgttccgc agccccgatc
     2941 aggctgagca acagcgatgc acgggcaccc gccaacgacc gggcgcccat gccggctggg
     3001 tgctactcct ccgccggtgg ctgataggcc aggtcgggtc tgggtcggac cagcagctgg
     3061 accagagatt ccacggttgc cgctcgctcc gcctcgagac gtcgcgcctc gcgctcgccg
     3121 aacgccccgc gaagccgctc cgatacggac atgacagggg cttcgtccag ctgggcatag
     3181 ctggtgggca cagagaagac gtaggtgttc tgggacccga agacgggcgc aaaggtcgcg
     3241 tagcgaacct caggcgccac ttcccgggtg gcttccgcca ccttggccac ataggcttca
     3301 aagcgctcgt tctcgcccgc tctcaccgtt gcctgcagcc agatcacccg ctgccccact
     3361 ggcgaatccg gcatgacggc cagatcgggc cgttgcaccc aaacacccct ctcgacgctg
     3421 gccactacct catggtcggg cgtgagcttg gcaatttctt ccgggccgaa agcctcggcg
     3481 atcgggttca tcggcgtctc gtccatggcg gtcatgctgc cgaacgtgcg cgccagcgtg
     3541 taggagcgcg ggttgcccat ctcctgctgc caggcgaacc aatgccgttt cgtgtccagc
     3601 ttggcgtggg cttcggccgc ccgcttgatg aaggtctcga aacggttctg cgcgccggga
     3661 tgaccgctca ccgtcaggtt ggtagcggca acgtacatgg gcgtcggcgc ggcctcttcc
     3721 gcacccgcca gcagcggtgc cgtcatcagg ctcagcgcca gaattattct tttcatgatc
     3781 gctcgtcctc gtatgctttg gttgtgtcag tccgggtgca aacac
//
LOCUS       DBNHQR010000329         2124 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_284952, whole genome shotgun sequence.
ACCESSION   DBNHQR010000329 DBNHQR010000000
VERSION     DBNHQR010000329.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_284952"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..1526)
                     /locus_tag="AC16VR_07335"
     CDS             complement(12..1526)
                     /locus_tag="AC16VR_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6011007.1"
                     /translation="MQQFDMVIVGAGVSGIGAACHFREKCPGKTFAILESRDAIGGTW
                     DLFRYPGIRSDSDMHTFGYNFKPWRNPKAIADGPSIREYVREAAREHDVDPHIRFNHR
                     LRSARWSSDSATWTLEVETPDGVVEFSCNFLFMCGGYYSYAGGYTPDFPGMADFEGPV
                     VHPQQWPDELDYSSKRVLIIGSGATAVTLLPAIAERAAHVTMLQRSPTYMVSRPDRDA
                     IANRLRRIFPEKVAYAITRWKNTTLQQFFYRRTRVNPEWVKDRLLRGVRAQLGPDFDV
                     QTHFTPSYNPWDQRLCLVPNGDFFRALRQGKATVVTDLIESFTSSGVRLKSGQTIEAD
                     VIVTATGLNLEVMSGVEFWVDEAKVNFPDTFTYKGMMFSGVPNLVQTFGYINASWTLR
                     ADLTSEYVCRLVNRMDELGVSQVAAQLREQDRDMPAKQWIDDFTPGYMQRSMHLFPKQ
                     GEKDPWRNTQNYAQDKKAIRHAPLEDGVLTFCNPDSAAPRVAGRASVARDQDAA"
     gene            complement(1637..>2124)
                     /locus_tag="AC16VR_07340"
     CDS             complement(1637..>2124)
                     /locus_tag="AC16VR_07340"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HIR6011008.1"
                     /translation="QTGWKRARATLRRDLGISTGKVRRSDASFKSLHGVVITNMVLFA
                     VLLACGHPALYLLWIGAWFTTYSLVMRIRSIAEHAMISDPGDPLNNTRTTKASWLARL
                     FVAPNRVNFHLEHHLLASVPLYHLPKMQRWLKESGFYGDKDCFSHGYLDVVRKATVPA
                     A"
BASE COUNT          384 a          663 c          688 g          389 t
ORIGIN      
        1 gggcggtgcg gtcaggccgc gtcctgatca cgagccacgc tcgcccttcc ggcgactctc
       61 ggcgcggccg agtccggatt gcagaaagta aggaccccgt cctccagtgg agcgtgcctg
      121 atggcctttt tgtcctgggc ataattctgg gtgttgcgcc agggatcttt ctcgccctgc
      181 ttcggaaaca gatgcatgga gcgctgcatg tatcccggcg tgaagtcgtc gatccactgc
      241 ttggccggca tgtcacgatc ctgctcgcgc aactgcgcag cgacctggga aacaccgagc
      301 tcgtccatgc ggttgacaag cctgcacacg tattctgagg taaggtcagc gcgcaacgtc
      361 caggaggcgt tgatgtagcc gaacgtctga accaggttcg gcacgccgga gaacatcatg
      421 cccttgtagg tgaacgtgtc cgggaagttg actttggcct cgtcaaccca gaactcgacg
      481 cccgacatca cctcgagatt caggcccgtc gccgtgacga tgacgtcggc ctcgatggtc
      541 tgcccggact tcagcctgac cccggaggag gtgaaggact cgatcaggtc ggtcaccacg
      601 gtggccttgc cctggcgcag ggcgcggaaa aagtcgccgt taggcaccag gcagagccgc
      661 tggtcccacg ggttgtaact cggcgtgaag tgggtctgca catcgaagtc cggaccgagc
      721 tgtgcccgca ctccacggag cagacgatcc tttacccact ccggattcac cctggtgcgg
      781 cgatagaaga actgctgcag ggtcgtgttc ttccagcggg tgatcgcata cgccaccttc
      841 tccgggaaga tccggcggag ccggttggcg atggcatccc gatcgggacg ggaaaccatg
      901 taggtcggcg atctctgcag catggtgacg tgcgctgccc gctcggcgat cgccggcagc
      961 agcgtcaccg ccgtggcccc gctgccgatt atcagcacgc gcttgctcga atagtcgagc
     1021 tcatccggcc actgctgggg gtgaacgacg ggaccctcga aatcggccat gcccgggaag
     1081 tcgggcgtgt agccgcccgc gtagctgtag tagccgccgc acatgaagag aaagttgcag
     1141 gagaactcca ccacgccatc gggggtttcc acctcgagcg tccaggtagc cgagtccgag
     1201 ctccagcggg cggagcgcag gcggtggttg aagcggatgt gaggatcgac gtcgtgctcc
     1261 cgcgccgctt cccgcacgta ctccctgatg gacgggccgt cggcgatggc cttcggattg
     1321 cgccagggtt tgaagttgta cccgaacgtg tgcatgtcgc tgtcggagcg gattcccgga
     1381 tagcggaaca ggtcccaggt acccccaatg gcatcgcgcg actcgagaat ggcaaacgtc
     1441 ttccccggac atttctcccg aaagtggcag gcggctccga tgccggatac acctgctccc
     1501 acgatgacca tgtcgaattg ctgcatcttg aagctcacct caaccctgcc ggcccgatgc
     1561 cgacgcccac gtcaatgggc ggatcacaaa ggggcaggat tgtgccgctt gcaaggctca
     1621 tttgcacccc gaccgtttac gcggcgggca ccgtcgcctt cctgaccacg tccaggtagc
     1681 cgtgagagaa gcagtccttg tcgccgtaaa agccgctttc cttcagccaa cgctgcatct
     1741 tcggcaggtg atagagcgga acgctggcaa gcaggtggtg ctcgagatgg aagttcacgc
     1801 gattgggtgc gacgaacagg cgtgcgagcc agctcgcctt cgtggtccgc gtgttgttga
     1861 gcgggtcgcc gggatcggag atcatcgcgt gctcggcgat cgagcggatc cgcatcacga
     1921 ggctgtaggt cgtgaaccag gcgccgatcc agagcaggta cagcgccggg tggccgcagg
     1981 ccagcagcac ggcgaacagc accatgttgg tgatgaccac gccgtgcagg ctcttgaagc
     2041 tggcgtccga gcgcctcacc ttgcccgtgc tgatcccgag atcgcggcgc agcgttgcgc
     2101 gcgcgcgctt ccagccggtc tgac
//
LOCUS       DBNHQR010000330         2776 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_158132, whole genome shotgun sequence.
ACCESSION   DBNHQR010000330 DBNHQR010000000
VERSION     DBNHQR010000330.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_158132"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="AC16VR_07345"
     CDS             complement(<1..272)
                     /locus_tag="AC16VR_07345"
                     /inference="COORDINATES: protein motif:HMM:NF013188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter domain-containing
                     protein"
                     /protein_id="HIR6011009.1"
                     /translation="MHSTGIVESFFLIFTGAAVLATAALYSRQPLLVAYIAVGALLGP
                     HGFKLISDSNLLSEISEIGIIFLLFLVGLDLQPSKLKNMVGESLLTA"
     gene            complement(236..742)
                     /locus_tag="AC16VR_07350"
     CDS             complement(236..742)
                     /locus_tag="AC16VR_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017674823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HIR6011010.1"
                     /translation="MNAQLDYIDQPAAQMVSGPGAVPGPARLVAAEQWTPGSSDGLLL
                     AVDAEPEQRFAGAELIAIDFPAFTDGRGLSLAVLLRTRLGFRGELRAVGDIRADMLHD
                     LYRCGFDSFSMSGEGSPVDADGVTKTTAFDALAPYSDCYQASVRQPRPAYRRVERCIA
                     QELSSPSF"
     gene            complement(726..2387)
                     /locus_tag="AC16VR_07355"
     CDS             complement(726..2387)
                     /locus_tag="AC16VR_07355"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011267700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HIR6011011.1"
                     /translation="MYRYDDFDRQFIAERVAHYREQTTRYLAGELSESEFLQLRLRNG
                     LYIQRLAPMLRVAVPYGTLSSGQLRKLAHIARHYDRGYGHLSTRQNMQFNWPELAEVP
                     DILAELAEVDMHAIQTSGSCIRNVTTDQFAGVAADEEVDPRPYCELIRQWSTNHPEFD
                     WLPRKFKIAVTGAASDRAAIAVHDIGLRARREDAEGIVFDVFVGGGLGRTPVIAAEIR
                     KSLPVRHLLTYLEAIMRVYNRYGRRDNKYKARIKILVRAMTAEGFRDRVDAEWAHLKG
                     SESTLTDEEIRRIEAFFTPHEYPPLNDADLGPARLEDPAFAAWLRHNVSPHRVPGYAI
                     VTLSTKVTGVPPGDVSDVQMEAVADWADEYGFGELRLGHEQNFVLPDVPQSRLRELWR
                     KTAALQLGVANVGLISDIVCCPGGDYCSLANAKSIPVAEAIQQRFDDYDYQHDLGELD
                     LNISGCMNACGHHHVGHIGILGVDKKGAEYYQISLGGHSGAGGDEASLGEIIGPSFSR
                     AEVPATIGRILDVYLKRRIDDEPFLHCYRRIGIQPFKDHVYERAA"
     gene            2499..2708
                     /locus_tag="AC16VR_07360"
     CDS             2499..2708
                     /locus_tag="AC16VR_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011012.1"
                     /translation="MLRTVFFGRLARRALGTINSLVRSTMDDTPTDDARSEADRKADA
                     WAVMIIFWALVAGAVHYVSGWTFDF"
BASE COUNT          499 a          835 c          927 g          515 t
ORIGIN      
        1 gcggtcagca gggattcgcc caccatgttc ttcagcttgc tgggttgaag gtccaggccc
       61 acgagaaaga gcagaaagat gatgccgatc tcggagattt ccgaaagcag gttagagtct
      121 gaaatcagct tgaacccgtg aggtcccagc agagcaccga cggcgatgta ggcgacgagc
      181 agcggctggc gcgagtacag ggccgcggtg gctagcaccg cggcgcccgt gaatatcaga
      241 aagaaggact cgacaattcc tgtgctatgc acctctcgac ccttcggtaa gctggacggg
      301 gctggcgtac cgaagcttga tagcaatccg agtagggtgc aagggcgtcg aaggccgtgg
      361 tcttggtcac gccgtcggcg tctacggggc tgccctcgcc ggacatggag aagctgtcga
      421 agccgcaacg ataaaggtcg tgcagcatgt cagcccgaat gtcgcccacg gcgcgcagct
      481 cgcctcggaa accgagacgc gttcggagca gcacggccag cgaaagtccg cggccgtccg
      541 tgaatgccgg aaagtcgatg gcgatcagtt ccgccccggc aaaccgctgc tccggttcag
      601 cgtcgacggc gaggagcaga ccgtcggacg atccgggcgt ccattgctcg gcggcaacca
      661 gcctcgccgg acccggcacg gctcccggtc cagacaccat ctgcgcggcg ggttggtcga
      721 tgtagtcaag ctgcgcgttc atagacgtga tccttgaaag gttggatgcc gatgcggcga
      781 tagcagtgca ggaaaggctc gtcgtcgatg cgccttttca ggtagacgtc gaggatcctg
      841 ccgatggtcg cgggaacctc ggcgcgggaa aaggatgggc cgatgatctc gccgagagac
      901 gcttcgtctc cgccggcacc cgaatgcccg cccagcgaga tctggtagta ctccgcgcct
      961 ttcttgtcga cgcccaggat gccgatgtga cccacgtggt ggtgcccgca ggcgttcatg
     1021 cagccggaga tgttcaggtc gagctcaccc aggtcgtgct ggtagtcgta atcgtcgaaa
     1081 cgctgctgaa tggcttccgc aacgggaatg gacttggcgt tggccagaga gcagtagtcg
     1141 ccgccggggc agcagacgat gtcggaaatc agcccgacgt tggcgacgcc cagctggaga
     1201 gccgcggtct ttcgccacag ttcccgaagc cggctctggg ggacgtcggg cagcacgaag
     1261 ttctgctcgt ggcccaatcg cagctccccg aacccgtact cgtctgccca gtcggccacg
     1321 gcctccatct gcacgtcgga aacgtctccc gggggcacgc ccgtcacctt cgtggacagc
     1381 gtaacgatgg cgtagccggg tacccggtgg ggagagacgt tgtggcgcag ccaggcagca
     1441 aaggcgggat cctccaggcg cgcgggtccc agatcggcat cgttcagcgg cggatactcg
     1501 tggggggtga aaaaagcctc gatgcggcgg atttcctcat cggtcagcgt gctttccgag
     1561 cccttcagat gggcccactc ggcatccacc cggtcgcgga aaccctccgc ggtcatggcc
     1621 cgtaccagaa tcttgattct ggccttgtac ttgttgtctc tgcggccgta gcggttgtag
     1681 acgcgcatga tggcctcgag gtaggtcagc agatgcctca ccggaagcga cttgcggatc
     1741 tcggcggcga tgacgggcgt gcgcccgagg ccgcccccca cgaacacgtc gaacacgatg
     1801 ccctcggcgt cctcgcggcg cgcgcgcaag ccgatgtcgt ggacggcgat ggctgctcgg
     1861 tcggaagcag ccccggtgac ggcgatcttg aacttgcgcg gcagccagtc gaactccggg
     1921 tgattggtcg accactggcg gatgagctcg cagtacggtc tgggatcgac ttcctcgtca
     1981 gccgcgacgc cggcgaactg gtcggtcgtc acgttgcgta tgcagctgcc gctggtctgg
     2041 atggcgtgca tgtcgacctc ggccagctct gcgaggatgt cgggtacctc ggcgagctcg
     2101 ggccagttga actgcatgtt ctgacgcgtg ctgaggtgac cgtaaccgcg gtcgtagtgg
     2161 cgcgcgatat gggccagctt gcgcagctgg ccggagctga gcgtgccgta ggggacggcc
     2221 acccgcagca ttggagcgag tcgctgaatg tacaggccgt ttcggagcct gagctgcaga
     2281 aactcgctct cggacagctc gccggcaaga tagcgggttg tctgctcgcg gtaatgggca
     2341 acgcgttccg caatgaactg cctgtcgaag tcgtcgtatc gatacatctg gctggctggt
     2401 aatcggcgct cagcgcgaag cgcaggccgc gtctcgtcat ctttgaggca ggcgaatcta
     2461 acattcggcc cttgccgctc gaagtaaccg ttcggactat gcttagaacc gtctttttcg
     2521 gtagactcgc gcgccgtgcg ctgggcacga tcaactcgct ggtgaggagc acgatggacg
     2581 acacccccac agacgatgca agatctgaag cggatcgcaa ggctgacgcc tgggccgtga
     2641 tgatcatttt ctgggccctt gtcgccgggg ccgtgcacta cgtctccggc tggaccttcg
     2701 atttctagcg atcgaggttg ggcctgagca acttctccgc ttccagggcg gtaattcctt
     2761 tcctgctggc gtagtc
//
LOCUS       DBNHQR010000331         4508 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_728291, whole genome shotgun sequence.
ACCESSION   DBNHQR010000331 DBNHQR010000000
VERSION     DBNHQR010000331.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4508)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_728291"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1235
                     /locus_tag="AC16VR_07365"
     CDS             <1..1235
                     /locus_tag="AC16VR_07365"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045240 - alpha-ketoacid dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HIR6011013.1"
                     /translation="KETLDVDAEVAGTVKHLVGEGVTMKPGDVVGYVYAPGEEIPDDL
                     PAAGAATPAPAAETAETTAPAAAAPAAITRGDGERILSSPAAGRLAGELGVDISTLAG
                     TGPGGRIVEADVQAAAAGAGEERGSRSSPMARKVARELGVDIATVTGTGPGGRITKED
                     VELAAKAPPAAAPAAREAVASRGDQTVPVRGMRKTIAARMHESLQSMAQLTMDMDVIM
                     DDAVKLRGQLIEEWADEGVRPTYTDLVIRAAAKALEKHPLMNSVFGETEMTLKGEVNV
                     GMAVALEEGLIVPVVRHANVLNMKELARESSRLAAACRDGNLTPDDLQGGTFTVSALG
                     MFGVDSFTPIINSPQVGILGVNRLRDDVAWDGAVPVKRQAMRLSLTWDHRALDGAPAA
                     QFLATVRDLLEAPYRLLV"
     gene            1245..2129
                     /locus_tag="AC16VR_07370"
     CDS             1245..2129
                     /locus_tag="AC16VR_07370"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015649522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA dioxygenase family protein"
                     /protein_id="HIR6011014.1"
                     /translation="MTITVRPLTPVIGAEVSGADLTDLGDQTFADIDRALTEHLVLFF
                     RDQPRLEPDVQIAFARRFGDLHIHPAAPHLEGYPEIFVIHTDRNSKVANGNGWHTDVS
                     CDEQPPMATMLQLHVLPATGGDTLFASMYGAYETLSAAMRQFLAGLTATHASEHVYRG
                     RYEDRGVEDAGRTYPSAVHPVVRTHPRSGRQALYVNPSFTTRINELSGQESRTLLKML
                     YRHQQRAEFQVRFQWTPNAIALWDNRCTQHFALWDYWPAERKGHRVTIGGERPFYEPG
                     AEKPQAAGLRLASELSSQ"
     gene            2146..3711
                     /locus_tag="AC16VR_07375"
     CDS             2146..3711
                     /locus_tag="AC16VR_07375"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6011015.1"
                     /translation="MTHFPQRIAADRGDAPALTDERGSTSWAELNARSNQLVHAFRAM
                     GLVPGDAIAIYAGNCREYYEVMMAANHAGLIYVPVNWHFSPEELAYVIDNAGARLLVT
                     EGQFLANAEAAVARGETPGLEQCVVIRPEADTTAFTDYEAFIGDQSTAEPDDQGAGGP
                     MFYTSGTTGRPKGVKSSTFAPGGPLAVLEMIGSGLSQMLQIPTDGTTYICGPVYHSAQ
                     WAFSFLPLISGSHVVMRHRFDPAESLQVMDAHEVTNVHLVPTQFHRLLRLSDDERAGF
                     SGSSLQVVWHGAAPCPPDVKRRMIQWWGPVVAEYYGSTEGSIVTTASASQWLERPGTV
                     GKPSPLVEVTIRDDDGQPLPANESGQIYVKSLMGSDFEYHNEPAKTAEVHLEPGTYTF
                     GDIGYLDDDGYLYLSDRKIDMIISGGVNIYPAEIEAVLVNHPAVEDAAVFGIPNEEFG
                     EEVKAAIKLRPGQSGNEELSADIIGFCRDHLAHYKAPKSIDYEDEFPRHETGKLYKRL
                     LRDRYWQDADRKI"
     gene            3721..4332
                     /locus_tag="AC16VR_07380"
     CDS             3721..4332
                     /locus_tag="AC16VR_07380"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6011016.1"
                     /translation="MGLYAKHVLPRMIDVLCGQPPMTELRRRYVPQASGDVLEIGIGS
                     GHNLAHYSSRVSSVTGLDPAAELTVMAQERANGLHTDVKLLQVSGEEIPADDARYDSL
                     VCTWTLCSIPNVYAAVREMRRVLKPGGRLYFVEHGASPDLPVARWQRRIEPVWKKIGG
                     GCHLTRRADELLQDAGFKLAELDTGYLPGPRFAAFMIHGIAER"
     gene            complement(4329..>4508)
                     /locus_tag="AC16VR_07385"
     CDS             complement(4329..>4508)
                     /locus_tag="AC16VR_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011017.1"
                     /translation="LDSAPTPLQEVFRSRFDALEAWEKSMIIASLQRVAAMMDAEQLD
                     ASPMLAVGSPDPAAS"
BASE COUNT          796 a         1567 c         1480 g          665 t
ORIGIN      
        1 aaaaggagac tctggacgtc gacgccgagg tggccgggac ggtcaagcac ctcgtgggcg
       61 agggcgtgac catgaagccc ggcgacgtgg tcggctacgt ctacgccccc ggagaggaga
      121 ttcccgacga ccttcccgcc gccggcgcgg caacgcccgc gccagcagcc gagacggccg
      181 agaccaccgc ccccgcggcc gcagcaccgg ccgcgataac tcggggcgat ggggagcgca
      241 tcctgtcctc gccagccgcc gggcggctgg ccggcgagct cggcgtcgac atctcgaccc
      301 tggccgggac cggtccgggc ggacggatcg tcgaggcaga cgttcaggca gcagctgcgg
      361 gcgcgggcga agagcgcggc agcagatcgt cgcccatggc gcgcaaggtg gcccgggagc
      421 tcggtgtgga catcgccacg gtcaccggga cggggcccgg agggcgcatc accaaggaag
      481 acgtggaact ggccgccaag gcacccccgg cggccgcccc ggcggccagg gaggcggtgg
      541 cctcgcgtgg cgaccagacc gtgcccgtcc gcggcatgcg caagaccatt gccgcccgca
      601 tgcacgagag tctccagtcc atggcccagc tgaccatgga catggacgtg atcatggacg
      661 acgcggtcaa gctgcgcggc cagctcatcg aggagtgggc cgacgaaggc gtgcgcccga
      721 cgtacaccga cctggtgatc cgtgccgccg ccaaagccct ggaaaagcat ccgctcatga
      781 acagcgtgtt cggcgagacg gagatgacgc tcaaaggcga ggtcaacgtg ggcatggccg
      841 tggctttaga ggaagggctg atcgtgcccg tcgtccgcca cgccaacgtg ctcaacatga
      901 aagagctcgc ccgggaatcg agccggctgg cggccgcatg ccgggacggc aacctcacgc
      961 ccgacgatct tcagggcggc acgttcacgg tctcggccct gggcatgttc ggcgtcgact
     1021 cctttacgcc catcatcaac tcgccccagg tgggcattct cggggtcaac cggctgcgcg
     1081 acgacgtggc gtgggacggc gcggtgcccg tcaaacgcca ggccatgcgg ctgtcgctga
     1141 cctgggacca ccgcgcgctg gacggcgcgc cggcggctca gtttctggca acggttcggg
     1201 atctgctgga ggcgccctac cggcttctcg tctgaggtgt ccaattgacc atcaccgtca
     1261 ggcccctgac acccgtgatc ggcgcggagg tctcgggagc ggatctcacc gatctcggcg
     1321 accagacctt cgcggacatc gatcgggcgc tcaccgagca cctggtgctc ttcttccggg
     1381 atcagccgcg tctcgagcct gatgtccaga tcgcgttcgc ccgccgcttc ggcgacctgc
     1441 acattcaccc cgcagcccct catctggagg gctacccgga gatcttcgtc atccataccg
     1501 atcgcaactc caaggttgcc aacggcaacg gctggcacac tgacgtgtcc tgcgacgagc
     1561 aaccgcccat ggcaacgatg ctgcagcttc acgtgctgcc ggccaccggc ggcgacacgc
     1621 tgttcgccag catgtacggc gcgtacgaga ccctgtctgc ggcgatgcgg cagtttctcg
     1681 ccgggctcac ggcaactcac gcttcggagc acgtctaccg cggccgctat gaggaccggg
     1741 gcgtcgagga tgccggcaga acctacccca gcgccgttca tcccgtcgtg cgcacgcatc
     1801 cgaggagcgg ccgccaggcg ctctacgtga atcctagctt cacaacgcgg atcaacgagc
     1861 tgtccggtca ggagagccgc acgcttctga agatgctgta ccggcaccag cagcgggccg
     1921 agttccaggt gcgctttcag tggacgccga acgccatcgc cctgtgggac aatcgctgca
     1981 cgcagcattt cgcgctctgg gactactggc ccgccgagcg caaagggcac cgcgtcacca
     2041 taggcggcga gcgccccttc tacgaacccg gggctgaaaa gccccaggca gccgggctcc
     2101 ggctcgccag cgaactttcc tcccaatgaa ccacggaacc gaccgatgac gcactttccg
     2161 caacgaatcg ccgccgaccg gggagacgcc ccggcgctca ccgacgaacg gggcagcacg
     2221 tcctgggccg agctgaacgc ccggagcaac cagctcgttc atgccttccg cgccatgggg
     2281 ctcgtcccgg gcgacgccat cgccatctac gccggcaact gccgcgagta ctacgaggtg
     2341 atgatggccg ccaaccatgc cggattgatc tacgtgcccg tgaactggca cttttctccg
     2401 gaggagcttg cctacgtgat cgacaacgcc ggcgcgcgcc tgctcgtgac cgaggggcag
     2461 tttctcgcca acgccgaagc cgcggtggcc aggggcgaaa cgcccgggct cgagcagtgc
     2521 gtggtcatcc ggcccgaggc agacaccacg gcgtttaccg actacgaggc gttcatcggc
     2581 gaccagagca cggcggagcc cgacgaccag ggcgccggcg gacccatgtt ctacacctcg
     2641 gggaccaccg ggcgacccaa gggcgtgaaa tcgagcacct tcgcacccgg tggcccactg
     2701 gccgtcctgg agatgatcgg ctcgggcctg tcgcagatgc tgcagatccc cacggacggg
     2761 accacctaca tctgcgggcc cgtctaccac tccgcgcagt gggcgttttc ctttctgccg
     2821 ctgatcagcg gcagtcacgt ggtcatgcgc caccgcttcg acccggccga gagcctgcag
     2881 gtgatggacg cccacgaggt gaccaacgta catctcgttc cgacccagtt ccaccggctg
     2941 ctgcgcctga gcgacgacga gcgcgccggc ttctcgggat cgagcctgca ggtagtctgg
     3001 cacggcgcgg cgccctgccc gcccgacgtc aagcgacgca tgattcagtg gtgggggccc
     3061 gtggtcgccg agtattacgg ctccaccgag ggctccatcg tgaccacggc gagcgcttcg
     3121 cagtggctgg agcggccggg caccgtgggc aagccgtccc cactggtgga ggtgaccatc
     3181 cgcgacgacg acggccagcc cctgccggcc aacgagtccg gtcagatcta cgtcaaaagc
     3241 ctgatgggct ccgacttcga gtaccacaac gagccggcca agaccgccga ggtgcatctg
     3301 gagccaggca cctacacctt cggtgacatc ggctacctgg acgacgacgg ctacctctac
     3361 ttgagcgacc gcaagatcga catgatcatc tcgggcgggg tcaacatcta ccccgcggaa
     3421 atcgaggccg tgctggtcaa tcatcccgcg gtggaagacg cggccgtgtt cggcatcccc
     3481 aacgaggagt tcggcgagga ggtcaaggcc gccatcaagc tgcgcccggg acagagcggc
     3541 aacgaggagc tcagcgccga catcatcggc ttctgccggg accacctcgc ccactacaaa
     3601 gcgccgaaat ccatcgacta cgaggacgag tttccgcgcc acgagacggg taagctctac
     3661 aagcggctcc tgcgcgaccg ctactggcag gacgcggacc ggaagatctg aggaacgacc
     3721 atgggactct acgccaagca cgttctcccg cggatgatcg acgttctctg cggccagccg
     3781 cccatgacgg agctcaggcg ccgctacgtg ccccaggcca gcggcgacgt gctcgagatc
     3841 ggcatcggat cgggccacaa tctggcgcac tactcgtcca gggtgtcctc ggtaacgggc
     3901 ctggatccgg ccgccgagct gacggtcatg gctcaggaga gagccaacgg gctgcacacg
     3961 gacgtcaagc ttctgcaggt ctcaggcgag gagataccgg ccgacgacgc gcgctacgac
     4021 tccctggtgt gcacctggac cctgtgctcg atccccaacg tctacgcggc cgtgcgcgag
     4081 atgcgtcgcg tgctgaagcc aggcgggcgc ctgtacttcg tcgagcacgg cgcctcgccc
     4141 gacctgccgg ttgcccgctg gcagcgacgc atcgagccgg tctggaagaa gatcggcggt
     4201 ggctgccacc tgacgcgacg cgccgatgag ctgcttcagg acgccggctt caagctggcc
     4261 gagctcgaca ccggctacct tccgggcccg cgcttcgcgg cgttcatgat ccacgggatc
     4321 gccgagcgct agctcgccgc ggggtcaggg ctgccaacgg ccagcatggg tgatgcgtcg
     4381 agctgctcgg cgtccatcat ggccgccacc cgctgcagcg acgcgatgat catggatttc
     4441 tcccaggctt cgagagcatc gaatcggctt cgaaagactt cctgcaacgg cgtcggcgcg
     4501 gaatcgag
//
LOCUS       DBNHQR010000332         3453 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_728326, whole genome shotgun sequence.
ACCESSION   DBNHQR010000332 DBNHQR010000000
VERSION     DBNHQR010000332.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_728326"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="AC16VR_07390"
     CDS             complement(<1..327)
                     /locus_tag="AC16VR_07390"
                     /inference="COORDINATES: protein motif:HMM:NF014664.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR6011018.1"
                     /translation="MSRLQKIEPEDWAPELKAAINPENLTPLEQGLTRFFAHRPELTL
                     GLMQFGGALKTKRTLPDKLIELVRLRVAFFNQCRSCMAIRYTDALNDGVTEDLVCSLE
                     KPAEAGN"
     gene            complement(324..1685)
                     /locus_tag="AC16VR_07395"
     CDS             complement(324..1685)
                     /locus_tag="AC16VR_07395"
                     /EC_number="5.4.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR6011019.1"
                     /translation="MTTVAEARAELAARTAGSQEISEARAEILNSGLAANLEMPHPIF
                     IESGEGPFVFDADGNRYIDTSVGFGLHILGHRHPLIEQAIKDRTEKGWMFGIHSTSQM
                     KLAELLHEASPCAERVVFCNTGSEATMYAIRAARGYSGKEKIALFDGFYHGAHDYGMW
                     LTDPTSPREAPRKLPMGRGIPRALADLTLLLPYRHEAAFDLIRRHADELALVMVEGVQ
                     SSNPQPGAGEFLCELEAVCREAGVLFAVDEVITGFRVAYGGAQERFGVRPDLATYGKI
                     LGGGLPVGAITGRADVMEVFTGLGASNGVFSGGTFSGNPLTMEAGAAAVGYLRDHPDV
                     YRGLEQAGERLAGAVNGFCQERQIPAQMKSVGSMFHMFFQREPIRSMRDVTGASVAAE
                     KAFYLHALNRGVLVPGTQRAFLSAAHSDAEIDTLIDVFQTSLADVQAEGLFSARTDKG
                     EMR"
     gene            complement(1682..2647)
                     /locus_tag="AC16VR_07400"
     CDS             complement(1682..2647)
                     /locus_tag="AC16VR_07400"
                     /inference="COORDINATES: protein motif:HMM:TIGR03621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03621 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR6011020.1"
                     /translation="MAKPFRFGFQTFNAESGQSWADKARKAEDLGYSSFHLADHLLGP
                     GPALTAANHPIQNVAAIPAMAFAAAVTSTINIGCRVFCIDYHLPVVLIKSAMTIDMLS
                     NGRLELGLGAGWVKSEYEAVGIDFDEPRVRIDRLADVIAGVKAFRGEDLVDVHNDTLS
                     WKDFEGLPKPVGRAPIMVGGGSPRVLRLAGREADIVSLNFNNRMGMIGPDGVQMSSEQ
                     ETRKKIGWIREGAGDRFDDLEIEIGAYFTVVTDDAKPVVAGMAQMFGMSEDEMLRHPH
                     GLFGSVDAICDELQRRREEFGISYFTVGEDNLEAFAPVVARLSGK"
     gene            complement(2674..3420)
                     /locus_tag="AC16VR_07405"
     CDS             complement(2674..3420)
                     /locus_tag="AC16VR_07405"
                     /inference="COORDINATES: protein motif:HMM:NF018038.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HIR6011021.1"
                     /translation="MAERIIDVYMVANGLYHDIDYARSELLKLLGEHENVRVKVAANY
                     SDIEGIAASDFLITYCCHLIPSEEEQVALRDYVNSGKRWIALHGTNAILEFVEGGVDC
                     PETAPIFMETLGSQFMAHPPIQPFPVKVSDPEHELVAGIGEFETDDELYICKYHGEHH
                     ALLHTNFTGSCDGFINRDWPDDEPRVMYYLRKLGDGEVLYNCLGHCRGHYDMRPVTDY
                     YPAIERGSWDKPEYYELLRRSIKYCIDRVV"
BASE COUNT          587 a         1129 c         1112 g          625 t
ORIGIN      
        1 gttcccggct tcggccggtt tctccagcga gcagaccaga tcctcggtca cgccgtcgtt
       61 gagagcgtcc gtgtaacgga tcgccatgca gctgcggcac tggttgaaga acgcgacccg
      121 cagccgcacg agctcgatga gcttgtcagg gagcgtccgc ttggtcttga gcgcgccgcc
      181 aaactgcatc aggcccagcg taagctcggg ccgatgagcg aagaaccggg tcaggccctg
      241 ctccagcggg gtcaggttct cgggattgat cgcggccttg agctcaggcg cccagtcttc
      301 aggctcgatt ttctgtagtc gactcatcgc atttcccctt tgtctgttcg ggccgagaaa
      361 aggccctccg cctgcacgtc ggcaagcgat gtctgaaaca catcgataag agtatcaatt
      421 tccgcgtcgg aatgagcagc ggaaaggaac gcgcgctggg tcccgggcac cagaacgccc
      481 cggttcagcg cgtgcagata gaacgccttc tccgcggcga cgctcgcgcc ggtgacgtcg
      541 cgcatgctgc ggatgggctc ccgctgaaag aacatgtgaa acatgctccc cacggacttc
      601 atctgggccg ggatctgccg ttcctggcag aagccgttca ccgcccccgc cagccgctcg
      661 ccagcctgct cgagaccacg gtacacgtcg gggtggtcgc gaaggtagcc caccgcggcc
      721 gctcccgcct ccatggtgag cgggttgccc gagaacgtac cgccggagaa caccccgttg
      781 ctcgccccga gcccggtgaa cacctccatc acgtccgcac ggccggtgat tgcccccacg
      841 ggcaagccgc cgccgagtat cttcccgtag gtggcgaggt ccggcctcac gccgaatctt
      901 tcctgagcac cgccgtaggc gacgcggaaa cccgtgatga cctcgtcaac ggcaaacaga
      961 acgcccgctt cacggcagac ggcttcgagc tcgcatagaa actcgccggc cccgggctgg
     1021 ggattcgagc tctgaacacc ctctaccatg accagcgcaa gctcgtccgc gtgacggcgg
     1081 atcagatcga aggcggcctc gtgacgatac ggcagcagca gcgtcaggtc tgccagcgcc
     1141 cgcgggatgc cgcgccccat tggcagcttg cgcggcgctt cccgggggct tgtcgggtcg
     1201 gtcagccaca tcccgtagtc gtgggcaccg tgatagaagc cgtcgaacag agcgatcttc
     1261 tccttgccag agtacccacg ggcagcacgg atcgcgtaca tggtcgcctc ggaacccgtg
     1321 ttgcagaaaa ccacccgctc ggcgcagggg ctggcctcgt gcaggagctc ggccagcttc
     1381 atctggctgg tggagtgaat gccgaacatc caccctttct ccgtgcggtc cttgatggcc
     1441 tgctcgatca gcgggtgccg atggccgagg atgtgcaggc cgaagcccac ggaggtgtcg
     1501 atgtagcggt tgccgtccgc atcgaagacg aacggcccct cgccgctctc gatgaagatc
     1561 ggatggggca tctccaggtt ggcagcgagc cccgagttga gaatctcggc gcgggcctcg
     1621 gagatctcct gggatccggc cgttctggcg gccagctcgg cgcgcgcttc agcgaccgtg
     1681 gtcatttgcc ggaaaggcgc gcgaccaccg gcgcaaaggc ctccagattg tcctctccga
     1741 cggtgaagta ggagatgccg aactcctcgc ggcgacgctg cagctcgtcg cagatggcat
     1801 caacgctgcc gaagagaccg tgggggtggc ggagcatttc gtcctcggac atgccgaaca
     1861 tctgcgccat gcccgcgacc acgggcttgg cgtcgtcggt caccacggtg aagtaggcgc
     1921 cgatctcgat ctccagatcg tcgaagcgat cgccggcgcc ttcgcggatc cagccgatct
     1981 tcttgcgggt ctcctgctcg ctggacatct gcacgccgtc gggcccgatc atgcccatgc
     2041 ggttgttgaa gttcaggctg acgatgtcgg cctcccgtcc ggccaggcgc agcacccggg
     2101 ggctgccgcc gccgaccatg atgggagcgc gtcccacggg cttgggcaga ccttcgaagt
     2161 ccttccagga cagcgtgtcg ttgtggacat cgaccaggtc ctcgccgcgg aacgccttca
     2221 ccccggcaat gacgtcggca agccggtcga tgcgcacgcg gggctcgtcg aagtcgatac
     2281 ccaccgcttc gtactcgctc ttcacccagc cggcgcccag acccagctcg agccggccgt
     2341 tggacagcat gtcgatggtc atggcggact tgatcagcac cacgggcagg tgatagtcga
     2401 tgcagaacac ccggcaaccg atgttgatgg tgctcgttac cgccgctgca aacgccatgg
     2461 cggggatggc ggcgacgttc tgaatcgggt ggttggctgc ggtcagcgcc ggccccggcc
     2521 ccagaagatg atcggccaga tggaacgacg agtagccgag atcctcggcc ttgcgagcct
     2581 tgtctgccca gctctggccc gactccgcgt tgaacgtctg gaacccgaaa cgaaacggct
     2641 ttgccatgct cggctctccc tggcagctgc ccgctagacc acgcggtcga tgcagtactt
     2701 gatggaccgc cgcagcagct cgtaatactc gggcttgtcc cagctgccgc gctcgatggc
     2761 ggggtaatag tcggtgaccg ggcgcatgtc gtagtggccc cggcagtgtc cgagacagtt
     2821 gtacagcact tcgccgtcgc ccagcttgcg caggtagtac atcacccgcg gctcgtcgtc
     2881 cggccagtcc cggttgatga agccgtcgca ggatccggtg aagttggtgt gcagcagcgc
     2941 gtggtgctcg ccgtgatact tgcagatgta cagctcgtcg tcggtttcga actcgccgat
     3001 cccggccacg agctcgtgct cggggtcgct gactttcacg gggaagggct ggatgggcgg
     3061 gtgcgccatg aactgcgagc ccagggtctc catgaagatg ggcgcggtct ccggacagtc
     3121 cacgcctccc tcgacgaact cgaggatcgc gttggtgccg tgcagcgcga tccagcgctt
     3181 gcccgagttg acgtagtccc gcagggccac ctgctcttct tcgctgggaa tcaggtggca
     3241 gcagtaggtg atcagaaaat cgctggcggc gatgccttcg atgtccgagt agttggccgc
     3301 gaccttcacc cggacgttct catgctcccc gaggagcttg agcagctccg agcgggcgta
     3361 gtcgatgtcg tggtacagac cgttggcgac catgtacacg tcgatgatgc gctctgccat
     3421 ggtgttctcc cccagagatc aggttgctgt agc
//
LOCUS       DBNHQR010000333         1231 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_411139, whole genome shotgun sequence.
ACCESSION   DBNHQR010000333 DBNHQR010000000
VERSION     DBNHQR010000333.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_411139"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1231
                     /locus_tag="AC16VR_07410"
     CDS             <1..>1231
                     /locus_tag="AC16VR_07410"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HIR6011022.1"
                     /translation="LKRADRTTAEAPIIVCNEEHRFMVAEQCRAIGQSWRRIILEPEG
                     RNTAPAIALAALAACEEAGPDSAPLLLVLPSDHLITDLDGFASAAELAARAAGEGALV
                     TFGVVPTSPETGYGYIEAPGAELGSGVVRVRSFVEKPDLATANEYLAAGDYFWNSGMF
                     LLNARAYLDELGRHSPLMLEKASLAFERGAEDLDFFRPGPEFLDCPADSIDYAVMEKT
                     DRAMVVPAGFDWSDVGSWSAIWEASDRDGNGNRLEGDVISVDTQNSFVSARDRLVGTI
                     GVSDLVVVETSDAVLVADKDRVQDVRAVVEQLKAAGRDEYVHHREVFRPWGSYESVGA
                     GERYQVKRIKVKPGASLSLQMHHHRAEHWIVVRGTAEVTRGDETITLAENQSTYIPLG
                     VTHRLANPGKLTLELIEV"
BASE COUNT          200 a          397 c          431 g          203 t
ORIGIN      
        1 cgctcaagcg ggcggaccgt accacggccg aagcgcccat catcgtgtgc aacgaagaac
       61 accgcttcat ggtcgccgag cagtgccggg ccatcggcca gtcctggcgg cgcatcatct
      121 tggagcccga ggggcgcaac acggcgccgg ccatcgcgct ggctgccctt gccgcctgcg
      181 aggaggcggg gcccgattcg gcaccgttgc tgctcgtgct gccgtcggat catctgatca
      241 ccgacctgga cgggttcgcg tctgctgccg agctcgccgc ccgggccgcg ggtgaaggcg
      301 cactggtcac gttcggcgtg gtgccgacca gcccggagac cggctacggc tacatcgagg
      361 cacccggcgc cgagttgggt tccggcgtcg tgcgggtccg atccttcgtg gagaagcccg
      421 atctcgccac ggcgaacgag tacctcgccg cgggcgacta tttctggaac agcggcatgt
      481 tcctgctcaa tgcccgggcg tatctggatg agctcggccg gcacagccct ttgatgctgg
      541 agaaggcgtc gctggccttc gagcgcgggg ccgaagatct cgactttttc cgaccggggc
      601 ccgagttcct cgattgcccc gcggactcca tcgactatgc ggtgatggag aagaccgacc
      661 gagccatggt ggtcccggcc ggcttcgact ggagcgacgt gggttcctgg agcgccatct
      721 gggaggcgtc ggatcgggac ggaaacggca accgcctgga gggcgacgtc atcagcgtcg
      781 atacgcagaa ctctttcgtc tcggcccgcg atcggctggt tggcaccatt ggcgtctcgg
      841 acctggtggt ggtggaaacc tcagacgccg ttttagtggc ggacaaggac cgggtccagg
      901 acgtgcgcgc ggtggtcgag cagctcaagg cggccggccg cgacgagtac gtccatcacc
      961 gcgaggtgtt ccggccctgg ggcagctacg agtccgtggg tgccggcgag cgttaccagg
     1021 tcaagcggat caaggtgaag ccgggcgcga gcctttcgct gcagatgcac catcatcgcg
     1081 ccgagcactg gatcgtggtg cgggggaccg ccgaggtcac ccgcggcgac gagacgatca
     1141 ccctggcgga aaaccaaagc acttacatcc cgctcggggt gacccaccgg ctggccaatc
     1201 cgggcaagct caccttagag ctcatcgagg t
//
LOCUS       DBNHQR010000334         6826 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_854975, whole genome shotgun sequence.
ACCESSION   DBNHQR010000334 DBNHQR010000000
VERSION     DBNHQR010000334.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6826)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6826)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6826
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_854975"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1020..1106)
                     /locus_tag="AC16VR_07415"
     tRNA            complement(1020..1106)
                     /locus_tag="AC16VR_07415"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1070..1072),aa:Leu,seq:caa)
     gene            1228..2685
                     /locus_tag="AC16VR_07420"
     CDS             1228..2685
                     /locus_tag="AC16VR_07420"
                     /inference="COORDINATES: protein motif:HMM:NF036848.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HIR6011023.1"
                     /translation="MSNTPILVGVAQVEQRIEDVREGEEPLAMMIDATRLAARDAGCP
                     GLLSEANSVRVIRGMWPYANPARAVGEAIGNPGAETALTPFGGNSVQSTLNHSAIDIR
                     DGVHDVIIITGAECGRSQAKAGRAGVDLNWSEVPGTPDRYIGEDMEMRCPAERGLGMG
                     RPIQMYPMMENHLRYRRGDTIEEQLIRISELWASFSAVAAENPHAWMREAKTAEQLRT
                     ISVENRPVSFPYPKLMNSNNNVDQGAALILCSTEKAKALGIPEEKWVYPWAGTDAHDT
                     YFVSNRDSMSRSPAIRLAGRRCLELTGLTPLDLAFVDVYSCFPVAVQVAAEEIGLDTT
                     RPLTITGGLTFAGGPLNNYVMHSIATMVEKLRGARGEKGLITANGGYLTKHAFGVYGT
                     EPPATPFQHEDLQPAVDATATRKVVTEWTGEATVESYTVMYKGDELAVGHLSCLTPDG
                     VRTWANCEDPDTLLAMTREEFCGRPVRLADRRAHF"
     gene            2660..3130
                     /locus_tag="AC16VR_07425"
     CDS             2660..3130
                     /locus_tag="AC16VR_07425"
                     /inference="COORDINATES: protein motif:HMM:NF018028.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HIR6011024.1"
                     /translation="MPTGGHISEATPTDANGQPGATRRHAGLLRRVGAMCYDALLVVA
                     LWLVTLFPIVALSNSAVTGATVRSLLFIELFVFFAYFWVSRGQTLGMLAWRLRLVPAA
                     PGEPVTLRQALLRFIGALAAFATLGLGYLWMYIDPERRTWPDMLSGTRVIHEPR"
     gene            complement(3135..4211)
                     /gene="lptG"
                     /locus_tag="AC16VR_07430"
     CDS             complement(3135..4211)
                     /gene="lptG"
                     /locus_tag="AC16VR_07430"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HIR6011025.1"
                     /translation="MLAVMDRHIGRTVLATTMMVLIAFLGLMTIFALIDELGSSRESY
                     GFGEALTYVALTTPRRIYEMLPYMVFLGALIGLGSLASNSEIVVLRAAGVSVWRVFLS
                     VAWPAFIVLLLGFLLGEFVAPRGEELAEAHKTRSRQDSEVIVLRGGYWYREANLYMNV
                     DGLDGQGGLIGLRQFWLDAEENLVRSVSASRAEYLPGADPHWMLFDVVETAFQDESTS
                     VQRSESARWDGNVDPRLLGVRVLVEPRKLSVTDLSYQIDYMTREGLNSGSYQLAYWGK
                     LLQPLSVLGLALLALCFVLGPLREVGMGVRLSVGVIAGLSFKYLQDLFAPMSYVYDLP
                     PPLAVALPIIVCWAIGLVGLRRVA"
     gene            complement(4215..5288)
                     /gene="lptF"
                     /locus_tag="AC16VR_07435"
     CDS             complement(4215..5288)
                     /gene="lptF"
                     /locus_tag="AC16VR_07435"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HIR6011026.1"
                     /translation="MAVAARYLNRELFWVFVVVVVVLLTVAVGGRFIGYLQDAALGKY
                     AAQGLLTIMWLRLPEFLQQLLPFAYYVALLLTVSRWHADREMTIVQNSGVGGGRLLRW
                     LGIPAVLLAALVGLLSMVITPANNAILEDFFQEQRANEEFTAVSPGIFRTFARGNRVT
                     YTESVSDDQRELQGVFMAERRPDRQDVTVWAESGRQVVDPDTGSRFLVLNNGTRYEGR
                     IGDRNYRVVEFGALGQRLALQPAAQRSTEVESLPTRTLMGAGRPDEIAELHWRYSLPL
                     LVLIATGIAVGLGRVKPRQGRFAKIVPGILVFVGYYVALVAVRNALEDGVWPPALGLW
                     PVHLAFLGVSVALLTAASRPAKI"
     gene            5346..6806
                     /locus_tag="AC16VR_07440"
     CDS             5346..6806
                     /locus_tag="AC16VR_07440"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003288270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HIR6011027.1"
                     /translation="MKYSTKTGTLAELSTPCLITGLKTARRVARELGHEVVFTAATSD
                     FKDSAGQVQVVGLPSSGPVRRILVTGGADDDLSAESYRKVIGAAATALKSVPARSALW
                     ALNHARVDGQDAYWKASAGIFALSTALYAFDEHKSTRNDDAGITIRTVQVHADARTRS
                     NVQRAVRHGNALKSGLDLARNLGNQPPNVCNPPYLVREARKLARRDKVTVTSLDEKRM
                     RELNMGAFLAVSAGSETPGRMIIVRYNGGRRQDAPVVLVGKGITFDTGGISLKPPAAM
                     DEMKYDMCGAATVLGATLAAVEAALPINLVTIVAAAENMPSGRATRPGDIVTTHSGKT
                     VEILNTDAEGRLVLCDALSYARRYEPKAIVDVATLTGACIVALGSHASAVYSKDDDLA
                     EELVNAGEWTGDRAWRMPLWDEYQAQLKSRFADVANIGGPQAGSVTAACFLSRFTDSM
                     RWAHMDVAGSAFQGGANKGATGRPVGLLFRFLIGQS"
BASE COUNT         1343 a         2223 c         2114 g         1146 t
ORIGIN      
        1 aacgagcatt acggcctccc tttgcagtag ccttgggtca cacgatggct cggccttgag
       61 caaacttcga ccggataata atggacgatg ggtcgccgca ttcggaattt ttctcaatcg
      121 ctggctaggc atggcttttg gccaacgtta gtcaggtacg tatacgttca attgcgtcgt
      181 ctgatcgact tcgagatctg tcgggtcgag tccggatcag aggatcctta cacgtggcca
      241 aagctggaag gatatgaggt acgcgcggtc gacgagcagg agtttcacga caatctctgc
      301 gaggaactaa gtgatgccag atttgacagc gagttccagc gcggagatct atgcattgcc
      361 gcgctctgcg agggtacgat cgtagcgtac gactttactt ctaagcaccc gtctcgcgtg
      421 aaggagggtt tggagtttac tttcccggac gaattttcct acgggatggt cacaacaacc
      481 gcaccggctc atcgaggccg aaagatcgcg cgagagttgt ggagagtcag tcgccaaatt
      541 cggttacaac gcgagggccg cgatgtgctt cgcatctggt acgtcaatgc gaccaatttg
      601 gagtcgcgag cagcgaacgt caatactggc acaaaggaga actttcaggg gtatgccggc
      661 tatctgcgac tgttcggacg ctggcacgtg ttcgcttctc cgagatgtag aaggtttggg
      721 gccgggtttc ggcagtccga tagctacact cccggcagtc ccaccactta acgtctgaag
      781 tccaaagagg caggtggcac agcggtcgcc catacgcttc gacattttta gaataatgaa
      841 aataaagctt gagggccaaa gaatgcgacg tgctggaagc cgagatatcg cgcctcagat
      901 caggggtgcg ttcacgagag ccgcgctgca actcgaagag gaaggcaggc cgatggggga
      961 gttagaggct tcgttcagca ttcaggtgaa cttcgtggag cccgaacctg catttttact
     1021 ggtgcctggg gtgggaatcg aacccacact tggttgccca aaccagattt tgagtctggc
     1081 gcgtctacca gtttcgccac ccaggcagcg atcgcggatt gttgcacagg ccgagaaagc
     1141 gcggcaatgg cacctttgcg acagcgcagc ttcctttcgg tatcatcccc gccccttaaa
     1201 agattcagtc aggatttctt cgagaacatg agcaataccc ccattctggt tggcgtggcc
     1261 caggtcgagc agcggatcga ggacgtgcgc gaaggcgagg agccgctggc gatgatgatc
     1321 gacgccacgc gactggcggc gcgggacgct ggctgccccg gcctgctcag cgaggcgaac
     1381 tccgtgcggg tgatcagagg catgtggcct tacgccaatc ccgcaagggc cgtgggtgaa
     1441 gccatcggca atccgggcgc ggagaccgcg ctcaccccgt tcggcggcaa cagcgtgcag
     1501 tcgacgctga accatagcgc gatcgacatt cgcgatggcg tccacgacgt catcatcatc
     1561 accggagccg agtgcggccg cagtcaggct aaggcggggc gagcgggcgt cgacctgaac
     1621 tggagcgaag ttcccggcac gcccgaccgg tacattggcg aggacatgga aatgcgctgc
     1681 cccgccgagc gcggtctcgg catgggtcgc cccatacaga tgtacccgat gatggaaaac
     1741 catctgcgct atcgccgggg cgataccatc gaggaacagt tgatccgtat ctccgagctg
     1801 tgggcgtcgt tcagcgccgt cgccgcggag aatccccacg cgtggatgcg cgaggctaaa
     1861 accgcggagc agcttcgaac gatctccgtg gagaaccggc ccgtctcctt tccctacccc
     1921 aagctcatga actccaacaa caacgtggat cagggcgcgg ccctcattct ctgctcgacc
     1981 gagaaagcaa aagcgctcgg catccccgag gagaagtggg tgtacccctg ggcgggcacc
     2041 gacgcccacg atacctactt cgtgtcgaac cgggacagca tgagccgctc gcccgccatt
     2101 cggctcgccg gccgacgctg cctcgagctg accggcctga cgccattgga tctggccttc
     2161 gttgacgtct acagctgctt cccggtggcc gtgcaggtcg ccgccgaaga gatcggcctg
     2221 gacacgacac ggccgctcac catcaccggg ggtctgacct tcgccggagg gcccctgaac
     2281 aactacgtca tgcactccat cgccacgatg gtggagaaac tgcgcggcgc ccggggtgag
     2341 aaaggcctga tcaccgccaa cggtggctat ctcaccaagc atgctttcgg cgtgtacggt
     2401 accgagccac ctgcaacgcc gttccagcac gaggacctgc agccggccgt cgatgccacg
     2461 gccacccgga aagtcgtcac ggaatggacg ggcgaggcca cggtggagag ctacacggtc
     2521 atgtacaagg gtgacgagct cgccgtcggg cacctgagct gcctgacccc ggacggtgtg
     2581 aggacctggg ctaactgtga ggacccggac acgctcctgg ccatgacccg ggaggagttc
     2641 tgcggcaggc ccgttcgact tgccgaccgg cgggcacatt tctgaggcta cgcctacgga
     2701 cgccaacggg caaccgggcg ccacgcgccg ccatgccggg ctgctgcggc gcgtcggcgc
     2761 catgtgctac gacgcgctgc tcgtcgtcgc gctctggctc gtcacgctgt tcccaatcgt
     2821 tgcgttgagc aactccgccg tcaccggcgc cacggtgcga tccctcctgt tcatcgaact
     2881 cttcgttttt tttgcctact tctgggtgag ccggggccag acgctcggca tgctcgcctg
     2941 gcgactgcgc ttggtaccgg ccgcacccgg ggaacccgtc accctgcgac aggcactgct
     3001 gcgcttcatc ggcgccctcg ccgcgttcgc caccctgggt ctcggatacc tgtggatgta
     3061 catcgacccc gagcgccgaa cgtggccaga catgctgtcc ggcacacggg tgatccacga
     3121 accccgctga tcgctcaggc cacgcggcgc agccccacca ggccgatggc ccagcagacg
     3181 ataatgggca gagccaccgc tagcggaggc ggcaggtcgt agacgtagct catgggcgcg
     3241 aagaggtcct ggagatactt gaaggacaaa cccgcgatga caccgacgct cagccgcacg
     3301 cccatgccta cctcgcgcaa agggcccagg acgaagcaga gcgcgagcag agcaaggccg
     3361 agcacggaga gcggctgcag cagcttgccc cagtaggcca gctgatagga acccgagttg
     3421 agcccctccc tggtcatgta gtcgatctga tagctcaggt cggtgaccga cagcttgcgg
     3481 ggctcgacga gcaccctgac ccccagcaga cgcggatcga cgttgccgtc ccagcgagcc
     3541 gattcgctgc gttgcacgga cgtggattca tcctgaaacg cggtttccac cacatcgaag
     3601 agcatccagt gcggatcggc gccgggcagg tactctgccc gggacgcgga caccgagcgc
     3661 accagattct cctctgcgtc caaccagaac tggcgcagac cgatgagccc gccctgcccg
     3721 tccaggccgt ccacgttcat gtaaaggttc gcttcccgat accagtagcc accgcgcaga
     3781 acgatcactt cggagtcctg tctgctgcgc gtcttgtgcg cctcggcgag ttcctccccc
     3841 cggggggcaa cgaactcacc gagcagaaac ccgagcagca gcacgatgaa cgccggccac
     3901 gccacgctca ggaatacccg ccatacagag acgccggccg cgcgcagcac gacgatctcc
     3961 gaattgcttg ccaggctgcc aagcccgatg agggcaccga gaaacaccat gtacgggagc
     4021 atctcgtaga tgcgccgagg cgtggtcagc gccacgtacg taagcgcttc gccaaaaccg
     4081 taggattcgc ggctgctgcc aagctcatcg atgagcgcga agatcgtcat cagtcccagg
     4141 aaggcaatca gcaccatcat ggtcgtcgcg agaacggtcc ggccgatgtg tctgtccatt
     4201 accgccagca tggatcagat cttcgctggc cgcgacgcgg cggtgagcag agcaacggag
     4261 acgcccagga acgccaggtg aacgggccag agccccagcg ctggtggcca gactccatcc
     4321 tccagggcat tgcggaccgc gaccaacgcg acgtaatacc ccacgaagac cagaatgccc
     4381 gggacgatct tcgcaaagcg cccctgccgc ggtttcacgc gccccagccc cacggcgatt
     4441 cccgtggcga tgagcaccag cagcggcagg gagtagcgcc aatgcagttc tgcaatctca
     4501 tccggccggc ctgcccccat cagcgtccgg gtcggaagag attccacctc cgtgctgcgt
     4561 tgggccgccg gctgcagggc gagccgctgc ccgagcgcgc cgaactccac gacccgatag
     4621 ttcctgtctc cgatgcgccc ctcgtaacgg gtgccgttgt tgagcacgag aaaccggctg
     4681 ccggtatccg gatccacgac ctgccgaccg gactcggccc atacggtgac gtcctggcgg
     4741 tctgggcggc gctctgccat gaacacgccc tgcagctcac gctggtcgtc gctgacggat
     4801 tccgtatacg tcacccggtt cccgcgggcg aaggttcgaa aaattccggg cgagaccgcc
     4861 gtgaactcct cgttggcgcg ctgctcctga aagaaatcct ccaggatcgc gttgttggcg
     4921 ggcgtgataa ccatgctgag caaaccgacc aaggccgcca gcaataccgc cggaatgccc
     4981 agccagcgca ggagccgccc accgcccacg ccgctgttct ggacgatggt catctcacgg
     5041 tcggcgtgcc accgggacac cgtgagaagc agcgcgacgt agtacgcgaa gggcagaagc
     5101 tgctggagga actccggaag cctgagccac atgatggtga gaagaccctg tgcggcgtac
     5161 ttccccagcg ccgcgtcctg aaggtagccg atgaaccgac caccgacagc gacggtgaga
     5221 aggacgacga ccacgaccac gaacacccag aacagctcgc gattaaggta gcgcgcggct
     5281 accgccatat gccaagtcct tgttttagcc taaaattgcg cgccgagcgt caacctgggc
     5341 caattatgaa gtattcgacc aagaccggca ccctggccga gctgtccact ccctgcctga
     5401 tcacgggctt gaagaccgcc cgccgggtag cccgggaact cggtcacgag gttgtgttca
     5461 ccgctgcgac ctcggacttc aaggacagcg ccggccaggt ccaggtggtc gggcttcctt
     5521 catccggacc tgtgcggcgt attctggtca ccggcggcgc cgacgacgac ctctcggcgg
     5581 agtcgtaccg taaagtcatc ggggcggctg ccaccgcgct gaagtctgtc ccggcgcgat
     5641 cggctttgtg ggcgctcaat catgcccggg tcgacgggca ggatgcctac tggaaagcca
     5701 gcgccgggat cttcgccctt tccaccgccc tgtacgcatt cgatgagcac aagtccacgc
     5761 gcaacgacga tgccggcatc acgattcgga cagtgcaggt gcacgccgat gcccgcaccc
     5821 gcagcaacgt ccagcgcgcc gtccggcacg gcaacgctct gaagtccggg ctcgaccttg
     5881 cccgcaatct gggcaaccag ccgccgaacg tctgcaatcc tccctatctg gtgcgcgagg
     5941 cccgcaagct ggccaggcgt gacaaggtga ccgtcacctc cctggacgag aagcgcatgc
     6001 gcgagctgaa catgggtgcc ttcctcgccg tctctgcagg aagcgaaaca cccggacgca
     6061 tgatcatcgt gcgctataac gggggccggc ggcaggacgc gcccgtggtg ctggtaggca
     6121 aaggcattac cttcgacacc ggcggtatca gcctgaagcc gcctgcggcc atggacgaga
     6181 tgaagtacga catgtgcggc gctgccacgg tacttggcgc caccctggcc gccgtggagg
     6241 cggccctgcc catcaatctg gtcaccatcg tcgcggccgc ggagaacatg cccagcgggc
     6301 gggccacgcg gccaggcgac atagtcacca cgcattcagg caagacggtg gaaattctca
     6361 acaccgacgc cgaggggcgc ctggtgcttt gcgacgcgct ttcctacgcg cgccgctacg
     6421 agccgaaagc catcgtggac gtggcaaccc tcaccggggc ctgcatcgtc gctctgggtt
     6481 cccacgcctc cgccgtgtac tcgaaagacg acgatctcgc cgaagaattg gtgaacgccg
     6541 gcgagtggac gggcgaccgg gcctggcgca tgcccctgtg ggacgagtac caggcccagc
     6601 tgaaaagccg cttcgccgac gtcgccaaca tcggaggccc ccaggcaggc tccgtcaccg
     6661 cggcctgttt cctatcccgg ttcacggact ccatgcgctg ggcacacatg gacgtggccg
     6721 gcagcgcatt ccagggcgga gcgaacaagg gagccaccgg ccggcctgtg ggtcttctgt
     6781 tccgcttcct catcgggcag tcttgacccc atgacccgca tcgact
//
LOCUS       DBNHQR010000335        10020 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1299522, whole genome shotgun sequence.
ACCESSION   DBNHQR010000335 DBNHQR010000000
VERSION     DBNHQR010000335.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10020)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10020)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10020
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1299522"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..1527
                     /locus_tag="AC16VR_07445"
     CDS             190..1527
                     /locus_tag="AC16VR_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011028.1"
                     /translation="MNIRNVSLALILAAVCVPSQATDTEEQQEFDSPLILKASEVLSA
                     ETRNGEHHEVREEIVNDGFMNTYVIDSPFGTFEAYGSSMLRTRINEIGALAELDGLSK
                     TKVFMEAAAQSAVRAVTAPVSAAATIVTNPIGTVTGLPGGLARMFNRQKRKVEGAVAT
                     TASAAKSATGADDSGEEASIAEQAGEGAVDLGKSLMGTTRAERRWAKKLGTDPYTSNE
                     TLRKGIADVAVVDSSGYFLAELAVPIGGIVDLVVDVNEAIWERDAYELRQMNLVKLQD
                     MGVAEEEATAFLDNVAISPTIQTLIINGLGLLETTAGRDAFLRQATLAEDEAESRFYL
                     QSLAMIAWLNQEGENVAAITVHGPVSIAVDTGGNRVAMLPVDHLTWNPTTVEAVGLWP
                     AQDPDVPSDGRVYISGDISALARSHLEQAGWSARSKIARDFFTYLSSQESDEP"
     gene            complement(1633..3171)
                     /locus_tag="AC16VR_07450"
     CDS             complement(1633..3171)
                     /locus_tag="AC16VR_07450"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008031994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004065 - arylsulfatase activity [Evidence
                     IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HIR6011029.1"
                     /translation="MRRILLAMGASLLLPAMGLAADKPNILVIWGDDIGQSNISAYTR
                     GMMGYPTPNIDRIAAEGILFTDYYGEQSCTAGRSSFIMGQSVFRTGLSKVGLPGAEEG
                     MQVEDPSIAGLLKDQGYATGQFGKNHLGDRDEHLPTNHGFDEFFGNLYHLNAEEEPEN
                     EDYPKNPEFRERFGPRGVIRSSADGQIEDTGPLTKKRMETVDDETVAAAMDFMERQHA
                     AGKPFFVWWSGTRMHFRTHVKDEMRGISGQDEYSDGMVEHDMHVGMFLDKLDALGIAD
                     NTIIHYSTDNGPHYNTWPDAASTPFRGEKNTNWEGGWRVPAAVRWPGVVKPGTVSNGI
                     VHHMDWLPTFLAAAGKTDIKEDLLDGYRSPAMKRRYRVHLDGYDLTDHLKDPQGVESP
                     RKEIFYFSDDGDLTALRYQDWKLIFMEQRVEGTLQAWAEPFVPLRVPLLFNLRRDPYE
                     RSQKTSNTYYDWLIDRVFLMVPAQVYVGQFLATFREYPPRQKAASFSLERVLEQLQEG
                     GDSR"
     gene            3460..3843
                     /locus_tag="AC16VR_07455"
     CDS             3460..3843
                     /locus_tag="AC16VR_07455"
                     /inference="COORDINATES: protein motif:HMM:NF028308.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RT0821/Lpp0805 family surface protein"
                     /protein_id="HIR6011030.1"
                     /translation="MAQRHSRILTLLLLVVVAAPATAFWGWMRGSAANEFKESDWDLF
                     KAEMRQALEDGADGERFDWSNPKTGNSGSIRILETAEVEGETCRKAAFRNVTAKGTQG
                     QSVHYLCRQENGAWQFAAASAFESG"
     gene            complement(3914..6337)
                     /locus_tag="AC16VR_07460"
     CDS             complement(3914..6337)
                     /locus_tag="AC16VR_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011031.1"
                     /translation="MDVEGGLVVIHAPQIRAWDDFEDIEGLAALELRTDDGAPGRFGT
                     IAFRARAVPDLEARVVHFDDIEVTSVKEQGEEISEEARALFVAAMDLGARDVPLDLVL
                     SHLADGVIPESTTGIRFEPPEILVSTAPAVIVLLHGEPALAPVGDRGALFAANTNWPL
                     FRNGAEGAWYLRDNDNWLTAADLDGPWAWAGELPASLVQLPETDDWRSTRAAAKAWSR
                     PPESEPPSVFVSTEPAELILIDGEPELVEIGTLGLSFVANTESPLFGYSERWYYLVSG
                     RWFTSRALDGRWSAVLELPKAFAQIPEDHEKADVLAAVPDTPAARMAALDAQIPEKTE
                     VPRDLSMPETVTYVGEPVFEPIPGTNLKRATNTAYSVIQAGADYYLCYSGMWYAASAP
                     EGPWRVTSHVPSQVYQIPPSSPVYNTTYVKVQEESPVSVTFGVTQGYSSVYVSNGVPV
                     YGTGWYYPPYYYYYGGYPIYFAYPYTYGSASFYNPATGTYGSVSRAYGPYGGWGYASA
                     YNPTTGTYGRAEAVWDHDEWYAVGEAYNPHTGRYYTTERYYDADDERWEIDSRFSGER
                     GYVDVSREFDEDSGRATIETSRGGEGTFSREKSDGGWNTSGEFETAGGRTVTGSGRYE
                     DGEGSSTLRGSEGGTGTIERSVDDGVVTRDGTFSKDGKTIETETTRDGTGRRTSFETS
                     EGTSGAVGGRGQQRTALAQTESGDLYAARDGNVYRKSDDGWSRHSDGSWETIPMPSRD
                     ADAGARSYGGGDYRQSASSAYRGGADLDADRYRGLDRDYSARQRGMTRAGSYGGQRGS
                     FGRLQGRRR"
     gene            6774..7487
                     /locus_tag="AC16VR_07465"
     CDS             6774..7487
                     /locus_tag="AC16VR_07465"
                     /inference="COORDINATES: protein motif:HMM:NF023197.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 family protein"
                     /protein_id="HIR6011032.1"
                     /translation="MKKNSRIARFALPLAVLLLGACASTTPTINTEAAPDEMSYDGLY
                     PVKGTRVTQAWARSDLDLRSYNKILLQGAGIQYRPVKSTGYRGTAGRSGKNEFPLDEQ
                     QKERVAAAASEAFRSELAKIKSLEIVSEPGPDVLIVRGALLDVVSRVPPEPIGRGNTY
                     LDSVGEATLVIELADSQSGSVLVRAVDRRAAQQTGWMTESTSVTNAAEVRRLFSGWAR
                     LVRTALEELTDDLDIGEAQ"
     gene            7585..9801
                     /locus_tag="AC16VR_07470"
     CDS             7585..9801
                     /locus_tag="AC16VR_07470"
                     /inference="COORDINATES: protein motif:HMM:NF025875.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6011033.1"
                     /translation="MVPIRAALLPLIVSFLSGCDARPPVATDTPDHELEPSTAALTYV
                     GSQQCAGCHAGQHDAWQRSHHRKAMEKPGSHSIVGNFDGASFVHDGTTTEFRAESGSY
                     VVYTDGENGEIARFPVAYTFGVEPLQQYLLPLPRGRLQALSVAWDSRPREAGGQRWFH
                     LYPEDRVDHTDVLHWTQGSQNWNYMCADCHSTNVRKGYDETLDEYDTSFSEVTVGCEA
                     CHGPASAHVDWALTGDGQAGLPSLGGQTAEINACAPCHSRRSQIAEGFRPDLAYLDHY
                     VPALLDEGLYHSDGQILDEVYVYGSFLQSRMHAQGVSCSNCHDPHSAELRATGNDVCT
                     GCHSEAGRADFPTLTKARYDASAHHFHSDGGPGSQCVDCHMPARTYMQVDDRRDHSFR
                     VPRPDLTVELGVPNACTGCHEDRPASWARDVLAERFGKAEREHYGAVFDAARRADPSA
                     ERALAALAEDEARPAIVRATALSLMTAYDRGASSFALSAGLRDESPLVRLGALKGAER
                     WPPDRRWRSAAHLLEDDYLAVRLEAVRVLAPAAGSLPAASRERLNRGVSEYASAQALH
                     GDRAEAHTNMAGVYLNLGEPSLAERALSRAIQINPRWVPALINLADLYRATGRDAAGG
                     GFLDRALSAAPDSGDVLLAKALWLVRQRSQDEALTLLARAAAVEPTSTRIAYVYAAGL
                     HSNDRTQEALEVLDTILATRPGNRQLLEAAAAMARDNGQDGRALGYLEQLKAADGL"
BASE COUNT         1796 a         3316 c         3217 g         1691 t
ORIGIN      
        1 cccgtcgtcg cctgacactg accataagct ttcccggaag agccctcgcg atggcgcgag
       61 ccacatgatt tccctggcgg atgcagccct cgatccaccc gactaagatg tgaagacccc
      121 gcagcgatgt tcccggcgag ggacctgccg gcatcagagc cggttctcga caacgcccaa
      181 ggattttcta tgaatatacg taacgtctcg ctggcgctca ttctagccgc ggtgtgcgtg
      241 ccatcccaag ccaccgacac cgaggagcag caggaattcg attccccctt gattctgaag
      301 gccagcgaag tgctcagcgc cgagacccgc aacggagagc accacgaggt acgcgaggaa
      361 atcgtcaacg atggcttcat gaacacctac gtcatcgact cgcctttcgg gacatttgaa
      421 gcctacggct cgtccatgct tcgaacgcgc atcaacgaga taggcgcgct ggccgagctg
      481 gacggcctgt cgaaaaccaa agttttcatg gaagcagctg cccagtcggc ggtaagggcc
      541 gtcaccgccc ccgtcagcgc cgccgcgacc atcgtcacga accccatcgg caccgtcacc
      601 gggctccccg ggggcctggc acgcatgttc aacaggcaga aacgcaaagt cgagggcgcg
      661 gtggccacga cggcgtcggc agcaaagtcg gcgacgggcg ccgacgactc cggggaggag
      721 gccagcatcg cggagcaggc aggcgagggg gccgtcgatc tcggcaaaag cctgatggga
      781 accacccgcg cggagcggcg ctgggccaag aagctgggta ccgatccata tacctccaac
      841 gaaactctca gaaagggaat cgccgacgtg gccgtcgtcg acagcagcgg ctatttcctg
      901 gccgagctgg cggtgcccat cggaggcatc gtcgatctgg tggtggacgt caacgaggcc
      961 atctgggaac gcgacgccta cgagttgcgc cagatgaacc tggtcaagct gcaggacatg
     1021 ggtgtggcgg aggaggaggc gaccgcgttt ttagataacg tcgccatcag ccccaccatt
     1081 cagaccctga tcatcaacgg tctggggctg ctcgaaacca ctgccgggcg ggacgccttc
     1141 ctgcgtcagg cgaccctggc cgaggacgag gccgaatccc gattctacct ccagagcctg
     1201 gccatgatcg cgtggttgaa ccaggagggg gagaacgttg ctgccatcac cgtgcacgga
     1261 cccgtctcga tcgccgtcga caccggcggc aaccgtgtcg ccatgcttcc cgttgaccac
     1321 ctgacgtgga acccgaccac cgtcgaggcc gtcggcctgt ggccggcaca ggaccccgac
     1381 gtcccttcgg acggccgggt ctacatttcc ggagacatct ctgccctggc gagaagccat
     1441 ctggagcaag caggctggtc ggcacgatcc aagattgccc gggatttctt cacgtatttg
     1501 agcagccagg agtcagacga gccgtagccc gccggtttcg aacgggagag cgtctgcgga
     1561 aaggacctgg cgcttcagga gaagcagcgg gcgaccgccg aatacggccg cccactgggt
     1621 cgatgaacgg cgctagcgcg agtcgccgcc ctcctgcagc tgctcgagga cccgctccag
     1681 ggagaagctc gccgccttct ggcgcggcgg gtactccctg aacgtggcca ggaactggcc
     1741 cacgtagacc tgcgccggca ccatgaggaa gacccgatca atgagccagt cgtagtaggt
     1801 gttggacgtc ttctgcgagc gctcgtaagg atcgcgcctc aagttgaaga gcagcggaac
     1861 acgcagcggc acgaagggct cggcccaggc ctgaagcgtg ccttcgacgc gttgctccat
     1921 gaagatcagc ttccagtcct gatatcgtag agccgtcaga tcgccatcgt cggagaagta
     1981 aaaaatctcc ttgcgcgggc tttccacccc ctggggatcc ttgaggtggt cggtgaggtc
     2041 gtagccatcg agatggacac ggtagcgccg cttcatggca ggggagcggt acccgtccag
     2101 aaggtcttcc ttgatgtcgg tctttcccgc cgccgccaga aatgtcggca gccagtccat
     2161 gtgatgaacg atgccgttac ttaccgtccc gggcttcacg acgccaggcc agcgcacggc
     2221 tgccggcacg cgccagccgc cttcccagtt ggtgttcttc tcgccccgga aaggcgtaga
     2281 ggcagcgtcc ggccaggtgt tgtagtgcgg gccgttatcc gtggagtagt ggatgatggt
     2341 gttgtcggca atgccgagcg catccagctt gtccaggaac atccccacgt gcatgtcgtg
     2401 ctcgaccatg ccgtccgagt actcgtcctg cccggaaata ccgcgcatct cgtccttgac
     2461 gtgggtgcgg aagtgcatgc gggttccgct ccaccagacg aagaacggct tgcccgcggc
     2521 atgctgccgc tccatgaaat ccatggccgc cgccacggtt tcgtcatcga cggtttccat
     2581 gcgtttcttg gtgagcggcc cggtgtcctc gatctggccg tcggcgctcg agcgaatgac
     2641 gccgcgcggg ccgaaacgct cgcgaaactc gggattcttc gggtagtcct cgttctcggg
     2701 ttcctcttcg gcgttcaggt gatacagatt gccgaagaac tcgtcgaaac cgtgattggt
     2761 gggcaggtgc tcgtctcggt cacccagatg gttcttgccg aactggcccg tagcgtagcc
     2821 ctggtccttc agcaggccgg ctatggaggg gtcctccacc tgcatgcctt cctccgcgcc
     2881 cggcagaccg accttcgaca gcccggtacg gaagacgctc tgacccatga tgaaggacga
     2941 gcgcccagcg gtgcagctct gctcgccgta gtagtcggtg aacaggatac cctcggccgc
     3001 tatccggtcg atgttgggcg tggggtagcc catcatgccg cgggtgtagg cactgatgtt
     3061 gctttgaccg atgtcgtcgc cccagatgac gagtatgttt ggcttgtcgg cagccagccc
     3121 catggcgggc aagagcaaag acgcgcccat cgcgagcaaa atgcgtcgca tgtgtaaact
     3181 ccctagcgtt tcaaaaatgg attttcgttg agcccgccca tagcgagctt cgaggcatct
     3241 gcagcgcgcc gcacatagca ttttcgtctc ggtccgtcgc gcgccgcccg catgataccc
     3301 gggtctcgtg gacaggcaaa ccggctaaac ctggcgcatc gagtcacctc accccctcgc
     3361 cgccggaagc gctgcgccgg ctgccaggcc gggatcggtg gagcagatcc gcgaccttag
     3421 gctagacttg actccttttt cgacggggtc gaaaacatca tggcgcagcg gcactcgcga
     3481 atccttaccc tgctcctgct ggtcgtcgtt gcggcgcctg ccacggcctt ctggggctgg
     3541 atgcgcgggt cggccgccaa cgagttcaag gagagcgact gggatctgtt caaagcggaa
     3601 atgcgccagg ccctggaaga cggcgctgac ggcgagcgct tcgactggag caacccgaaa
     3661 accggcaaca gcgggtcgat cagaattctc gagaccgccg aggtggaagg cgagacctgt
     3721 cgcaaggcgg cgttccgcaa cgtgaccgcc aagggtaccc aggggcaatc cgtccactat
     3781 ctttgccgac aggaaaatgg cgcctggcag ttcgccgcgg ccagcgcgtt cgaatccggc
     3841 tgatcccaat cgcccctgga ccgctcccga tccggcacct agcaaccgta tttccaggcc
     3901 gtaccggctg gtgctaacgg cgccggccct gcagccgacc gaagctgccg cgttgtcccc
     3961 catacgagcc ggcgcgggtc atgccgcgct gtcgggcgga gtaatcgcga tccaggccgc
     4021 gatagcgatc ggcatcaaga tccgcgccgc ctcgataggc gctagacgcg gactggcggt
     4081 aatcgccccc gccataggat cgggcgccgg catcggcgtc gcggctgggc atcgggatgg
     4141 tctcccagct gccgtcgctg tgacgcgacc agccgtcatc cgatttcctg tagacgttcc
     4201 cgtctcgcgc cgcgtacaga tcaccggact cggtctgcgc cagcgccgtg cgctgctgtc
     4261 cccggccgcc gaccgcgccg ctggtgcctt ctgaggtctc gaacgacgtg cgccgccccg
     4321 tgccgtcgcg ggtggtttcc gtctcgatgg tcttgccatc cttggaaaag gtgccgtccc
     4381 gggtcaccac gccatcatcc accgaccttt ctatggtgcc ggtaccccct tcggagccac
     4441 gcagcgtgga gcttccttcg ccgtcttcgt agcggccgct gccggtgacg gtgcggccgc
     4501 cggcggtctc gaactcgccg ctggtgttcc agcccccgtc gcttttctcg cgagagaagg
     4561 tgccttcccc gccccgggac gtttcaatgg tggcgcgacc ggagtcttcg tcgaactctc
     4621 tggacacgtc tacgtaacca cgctcgccgg agaaccggga gtcgatttcc cagcgctcgt
     4681 catcggcatc gtagtaccgc tcggtggtgt agtaccgccc ggtgtggggg ttgtaggcct
     4741 cgcccaccgc ataccactcg tcgtgatccc aaaccgcctc cgcccgaccg taagtgcccg
     4801 tggtcgggtt gtaagccgag gcgtaacccc agccgccgta aggtccgtag gccctcgaaa
     4861 cgctgccgta ggtgcccgtg gctgggttgt agaaagaggc ggagccatac gtgtacggat
     4921 aagcgaagta gatcggatag ccgccgtagt aatagtagta gggcgggtag taccagccgg
     4981 ttccgtagac gggcaccccg ttggagacgt acacgctcga gtagccctgg gtcacgccga
     5041 aggtcaccga gaccgggctt tcctcttgaa ccttcacgta ggtggtgttg tagaccggcg
     5101 agctgggcgg gatctggtag acctgggagg gcacgtgact ggtcacgcgc cagggccctt
     5161 cgggcgccga agccgcgtac cacatgccgc tgtagcacag gtagtagtcc gcgccggcct
     5221 gaatgaccga gtaggcggtg ttcgtcgcac gcttgaggtt ggtgcccggg atgggctcga
     5281 acaccggttc gcccacatag gtcacggttt ccggcatgct gagatcccga ggcacctcgg
     5341 tcttctcggg aatctgggca tccagagcgg ccattctggc tgctggcgta tcgggaaccg
     5401 cggccagcac gtcagccttt tcgtgatctt caggaatctg ggcaaacgcc ttgggcaact
     5461 cgagcacggc cgaccacctg ccatcgagcg ccctgctcgt gaaccagcgt ccggaaacga
     5521 ggtagtacca gcgctcggag tagccgaaca gcgggctttc ggtattcgcg acgaaggaca
     5581 ggccaagggt gccgatttct accagttccg gctcgccgtc aatcaggatc agctcggccg
     5641 gctcggtgga tacgaaaacc gatgggggct cgctttccgg cggccgcgac catgccttag
     5701 ccgctgcgcg ggtggagcgc cagtcgtccg tttccggcag ctgaaccaga gaggccggaa
     5761 gctcacccgc ccaagcccac ggaccgtcga gatcggcggc ggtgagccag ttgtcgttgt
     5821 cccgcaggta ccaggctccc tcggcgccgt ttctgaacag gggccagttg gtattggcag
     5881 caaacagcgc tccccgatcc cccacaggcg cgagcgccgg ctcgccatgc aaaaggacga
     5941 tcacggctgg ggcggtggaa acgagtatct ctggaggctc gaagcggatg cccgtggtgg
     6001 actccgggat caccccatca gccaggtggc tcaggaccag gtcgagcgga acgtcccggg
     6061 cccccaaatc catggccgcc acgaacagcg cccgtgcctc ctcggagatt tcttcgccct
     6121 gctccttcac cgaggtgacc tcgatgtcgt caaagtggac gacgcgggct tccaggtccg
     6181 ggacggccct tgccctgaac gcgatggtgc caaaccggcc gggggcgccg tcgtccgtgc
     6241 gcagctccaa cgccgcgagt ccctcgatgt cctcgaaatc atcccacgcg cgaatctgcg
     6301 gagcgtgaat caccaccaac ccgccctcga catccacgga cctcggaaac tgccctggcg
     6361 tcggcgcttc agtcttgccg gcagacgcgg tggcagacgc ggtggcagac gcggtggcag
     6421 acgggccagc cgccggctct gacgcctgag ccgaactcag catcagccac gacgggacgg
     6481 gcgacgcgct cacaagcaac agcgaactga tcaacgcaag gcctgttggt gcccagcggt
     6541 ccataacgtt cctcgggggg tctgcgcagg agaccgggca gtgtagtcga aagccgtgat
     6601 cgtgcccacg acgacaaccc gaacgcggtg actgcccagg ggacgcccag gctgcgggct
     6661 gcgaggccac ctgccagtag gccgccttgc tttcggttag cttgacgcta acatcggccc
     6721 ctcacgacat ggaaagcaac cgtccaccaa tgacaaaaaa gggggaatcc gcgatgaaaa
     6781 agaacagccg catcgccagg ttcgcgctgc cgctggccgt cctgctgctg ggggcctgcg
     6841 cgagcacgac accaaccatc aacaccgagg cggccccgga cgagatgtcc tacgacggcc
     6901 tgtatcccgt gaagggaacc cgggttaccc aggcctgggc ccgctcggac ctcgacctga
     6961 gaagctacaa caagattctt ctgcagggag cgggcattca gtatcgaccg gtcaagtcga
     7021 ccggataccg gggcaccgcc ggtcgcagcg gcaagaacga gtttcccctg gacgagcagc
     7081 agaaagagcg cgtcgccgcg gccgcaagcg aagctttccg cagcgagctt gcgaagatca
     7141 aaagcctgga gatcgtttcg gagcccgggc ccgacgtgct catcgtgcgc ggcgcgctgc
     7201 tcgacgtggt gtcccgggta ccgccggaac ccattggtcg aggcaacacc tacctggatt
     7261 ctgtcggcga ggcgacgctg gtcatagagc tggcggattc ccaaagcggc agcgtgctgg
     7321 tgcgcgccgt ggatcggcgc gccgcccagc agaccggctg gatgacggag tccacctcgg
     7381 taaccaacgc cgcggaagtg cggcgtctgt tcagcggttg ggctcgcctg gtgcgcacgg
     7441 ccctggagga gctgaccgac gatctggaca tcggcgaagc ccaatagctg agccatcttc
     7501 cgccctctcg ccccgggttc aggcgcatcg cgtcctcccg gggcgatggg tgacggttaa
     7561 cgctcgctga ccccgtcgcc tgccatggtc ccgatccgag ccgctctcct gcccctgatt
     7621 gtgagcttcc tgtcgggctg cgatgcccgc ccaccggtcg ccactgacac cccggaccac
     7681 gaactcgagc cctcgacggc ggcgctcaca tacgttggct cccagcaatg cgcgggctgt
     7741 cacgccggcc agcatgacgc ctggcagcgc tcgcaccatc gcaaagccat ggaaaagcca
     7801 ggctcccaca gcatcgtcgg caatttcgac ggcgcctcgt tcgttcatga cgggaccacc
     7861 accgagtttc gcgcggaatc gggttcctac gtggtgtaca ccgatggcga gaacggcgag
     7921 atcgcccgct tccccgtcgc ctacacgttc ggcgtcgagc cgctgcaaca gtatctgctg
     7981 cctctcccgc gcggtcgcct gcaggcgttg agcgtggcct gggactcgcg gccccgggaa
     8041 gccggcgggc aacgctggtt ccacctctat cccgaggatc gggtggatca cacggacgtt
     8101 ctgcactgga cccaggggag ccagaactgg aactacatgt gcgccgactg tcactccacc
     8161 aacgtacgca agggttacga cgagacgctg gatgagtacg acacgagctt cagcgaggta
     8221 accgtaggct gcgaggcctg ccatggcccg gcgtccgcgc acgtcgactg ggcgctaacc
     8281 ggtgacggcc aagccggcct tcccagcctt gggggtcaga ccgccgagat caacgcctgc
     8341 gcgccctgtc acagccgacg cagccagatc gccgaggggt tccggccgga tctcgcctac
     8401 ctcgatcact acgttcccgc cctgctggac gaggggctct accacagcga cgggcagatt
     8461 ctcgacgagg tgtacgtcta cggatcgttc ctgcagagca ggatgcatgc acaaggggtt
     8521 agctgcagca actgccacga cccgcactcg gccgagctgc gcgccactgg caacgacgtg
     8581 tgcactgggt gccactccga agcgggccgg gccgacttcc ccacgctcac aaaggctcgc
     8641 tacgatgcat ccgcccacca ttttcattcg gatggaggcc ctggctcgca atgcgtggac
     8701 tgccacatgc ctgcccgcac ctacatgcag gtcgacgatc ggagggatca cagcttccgg
     8761 gttccccggc ctgatctgac cgtcgagctg ggtgtgccga acgcctgcac cggctgccac
     8821 gaggatcgcc cggccagttg ggcgagggac gtgttggcag agcgctttgg caaggctgaa
     8881 cgcgagcact acggcgccgt tttcgacgcg gcccgtcgcg cggatccgag cgcagagcga
     8941 gcgctcgccg cgctggccga ggatgaggcc cggccggcca tcgttcgcgc caccgcgctt
     9001 tccctgatga ccgcttacga ccgtggcgcc agctcgtttg cgctgagcgc cggcttgagg
     9061 gacgaaagcc ccctggtgcg cctcggtgcc ctgaaagggg ccgagcgctg gccgcccgat
     9121 cgccgctggc gttcggcggc gcacctgctg gaggacgact acctcgccgt gcggctggag
     9181 gccgtccgcg tgctggcccc ggcagcaggc tcgctcccgg cggcatcacg agaacgcctg
     9241 aatcgcggtg tgtcggaata cgcttccgct caggcgctgc atggggatcg ggcggaggcg
     9301 cacacgaaca tggctggcgt ctatctgaac ctgggcgaac cgagcctggc cgaacgggcg
     9361 ctatcccggg ccatccagat caatccccgg tgggtgccgg ctctgatcaa cctggccgac
     9421 ctgtacagag cgacggggcg tgatgccgcg gggggcgggt ttctggaccg tgcgctcagc
     9481 gcagcacccg actctgggga cgtgctgctc gccaaggcgc tgtggctggt gcggcaacgg
     9541 tctcaggacg aggccctcac cctgcttgcc cgcgcagcag ccgtcgagcc gaccagcacc
     9601 cgaatcgcct acgtgtacgc cgccgggctt cactcgaacg accgaacgca ggaggccctg
     9661 gaggtgctcg acaccatcct cgccacccgc cctggcaacc gacagctgct cgaagcagca
     9721 gcggccatgg cccgggataa cggccaggat ggccgagcgc tcggatacct ggagcagcta
     9781 aaagcagccg acggtctctg agcgaaccct gcccgcctgg cggatcttac gcgcgccaac
     9841 ggctccccag aggactcaga aagagagggt gtagttcgcg atgaaagtgg tgaagtcggc
     9901 gccgattcgc gcgctgaccc cttccgtggc caccagctgc atcctgtgtc tgcggccaac
     9961 ggggaacgag aaagccaggc ccgagcgcca gttgttctga tgatcgcgac gctttacacc
//
LOCUS       DBNHQR010000336         2007 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_348685, whole genome shotgun sequence.
ACCESSION   DBNHQR010000336 DBNHQR010000000
VERSION     DBNHQR010000336.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_348685"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1596)
                     /locus_tag="AC16VR_07475"
     CDS             complement(<1..1596)
                     /locus_tag="AC16VR_07475"
                     /inference="COORDINATES: protein motif:HMM:NF012337.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase"
                     /protein_id="HIR6011034.1"
                     /translation="MIRLPVIVGFGGINPAGRASFDHAYRRLVIDALDEASRARTYRS
                     LASLMNIHDDPDEVSTRAYIDAHTLIRRIELFDPNEVPVQRTARLAHPEDEPIRFVLA
                     TRQLPERIPAGWQVSSLTEQQVLVTVDNELDVLLPDTRISRVSSAGQAPTGFDPGALY
                     QSRNHPRGLQLTVFGASDALRSTGIPIEELKQRVAPDQFAVYSGSAMGQLDADGYGGM
                     LQNPLMGKRPTSKNVALGLSEMPGDFVNAYVLGSVGDTAGIIGACATFLYNMQHGIEE
                     IRSGRKRVVMVGNSEAPVVPDVIEAYRVMGALAEDEALMALDSRESEPDHRRACRPFS
                     DNCGFTVAEASIFTVLMDDELALQLGARILGSVGGVYVNADGYKKSIPGPGIGNYLTV
                     AKAMSLSRKLLGDESLQHRTYMLAHGTGTPQNRVTESHILNELAKVFGIERWPVAAIK
                     AYVGHSMAPAGGDQLAAALGLWQHGWLPGITTIDHIADDVHDSNLNLPLEHLELGAEN
                     VDAAFVNSKGFGGNNATGLFLSPA"
     gene            complement(1744..>2007)
                     /locus_tag="AC16VR_07480"
     CDS             complement(1744..>2007)
                     /locus_tag="AC16VR_07480"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170199.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HIR6011035.1"
                     /translation="KADDVDVFEINEAFAMVTMLAIQTLNLDPSRVNVYGGACAQGHP
                     VGSSGSRLIVTLAHALERLNKKRGVAALCIGGGEATAIAIERP"
BASE COUNT          315 a          640 c          692 g          360 t
ORIGIN      
        1 cgccggtgac aggaacagcc cggtggcatt gttgccaccg aaacctttgg agttgacgaa
       61 ggcggcatcc acgttctcgg cgccgagctc caggtgctcc agcggcaggt tcagattgct
      121 gtcgtgaacg tcgtcggcga tgtgatcaat ggtggtgatg cccggcagcc agccgtgctg
      181 ccacaggccc agggcggcgg ccagctgatc gcctcccgcg ggcgccatgg agtggcccac
      241 gtaggccttg atggcagcta cgggccagcg ctcgataccg aagaccttgg ccagctcgtt
      301 gagaatgtgc gactcggtca cccggttctg tggcgtcccg gtcccgtggg ccagcatgta
      361 ggtgcggtgc tgcagggact cgtcgccgag cagcttgcgg gagaggctca tggccttggc
      421 caccgtgaga tagttgccga tgccggggcc cgggattgat ttcttgtacc cgtcggcgtt
      481 gacgtagacg ccccccaccg agccgaggat gcgggcgccg agctgcaatg ccagctcgtc
      541 gtccatcaga acggtgaaga tgctcgcctc cgccacggtg aacccgcagt tgtcggagaa
      601 cggtcgacag gcgcgccggt gatcgggctc ggactcccgg ctgtcgagcg ccatcaacgc
      661 ttcgtcctcg gcgagcgcgc ccatgacccg gtacgcctcg atgacgtctg gcaccacggg
      721 cgcctcggag ttgcccacca tcaccacgcg cttgcgcccg gagcgaattt cctcgatccc
      781 gtgctgcatg ttgtacagga aggtcgcgca ggcgccgatg atgcccgcgg tatcgccgac
      841 ggacccgagc acgtaggcat tgacgaagtc gccgggcatt tccgacagcc cgagcgcgac
      901 gttcttcgac gtggggcgtt tgcccatgag cgggttctgc aacatgccgc cgtagccgtc
      961 ggcgtccagc tgccccatgg cgctgcccga gtagacggca aactgatcag gcgcgacccg
     1021 ttgctttagc tcctcgatgg gtatgcctgt ggagcgcagg gcgtccgacg cgccgaacac
     1081 cgtgagctgg agcccgcgcg gatggtttcg cgactggtac agagcgccgg gatcgaagcc
     1141 ggtaggtgcc tggcctgcgc tgctgaccct ggagattcga gtgtcgggaa gcagcacgtc
     1201 gagctcgttg tctaccgtca cgagcacctg ctgctcggtg agggaagaga cctgccagcc
     1261 cgccgggatg cgctcgggca gctgccgcgt tgccagaacg aaacggatgg gctcgtcctc
     1321 cggatgggcc agcctggcag tgcgctggac gggaacttcg ttgggatcga acagctctat
     1381 cctgcggatg agcgtgtggg cgtcgatgta cgcccgggtg gagacttcgt ccgggtcgtc
     1441 gtgaatgttc atcaggctcg ccaggctgcg gtaggtgcgg gcccgggagg cctcgtcgag
     1501 agcgtcgatc accaggcgtc gataggcgtg gtcgaaggac gccctccctg ccgggttgat
     1561 gccgccgaac cccacgatga ccggaagccg gatcactcat actctccgtt gctggcaacg
     1621 cgcttggggt cgccggatgc aggatgcttc tcgcgcagcg cgcccgcttg cgggaggacg
     1681 cgcgctcgcc agatcaccgg agaattgtag ggagacgcct gcgccatttc cattagtgtg
     1741 acgtcatggc ctctcgatgg cgatggcggt cgcttcgccg ccgccgatgc agagcgcggc
     1801 cacgccccgc ttcttgttca ggcgctccag cgcgtgcgcc agcgtgacga tcaggcggct
     1861 gccgctggat cctacggggt gtccctgggc acacgcgccg ccgtagacgt tcacgcgcga
     1921 cgggtccagg ttcagcgtct ggatcgccag catcgtgacc atggcgaaag cctcgttgat
     1981 ctcgaagacg tccacgtcgt cggcctt
//
LOCUS       DBNHQR010000337         5441 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_665699, whole genome shotgun sequence.
ACCESSION   DBNHQR010000337 DBNHQR010000000
VERSION     DBNHQR010000337.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5441)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5441
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_665699"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="AC16VR_07485"
     CDS             <1..917
                     /locus_tag="AC16VR_07485"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR6011036.1"
                     /translation="PIGTLSGGQRQALTLLMATWLKPDLLLLDEHTAALDPKSAAQVI
                     RLTHEIIARERITTLMVTHSMQQAVDLGDRIVMMHQGQVLHDISGAAREQIRAEDLLS
                     RFEDLRRKEQLERSREGLLLEAQGIEELMEVFKAVIYVDERVDEREVELLREFAERWN
                     VPLPDLTSPPVTGEGIMAVRAAVAAYLRVSPPTEQASELLDVLQHFVHADETVTEEET
                     TVMDEVGAMVIRYVTSSSDQGRHQVVIVPQNEAQVGEARALVPEAEPTFMRGGKVISV
                     GRFFSAKYADMVCEGYVAKGFFTTRIEL"
     gene            1192..2943
                     /gene="glsA"
                     /locus_tag="AC16VR_07490"
     CDS             1192..2943
                     /gene="glsA"
                     /locus_tag="AC16VR_07490"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - L-glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HIR6011037.1"
                     /translation="MDRLAELFARVADSGVCTAATLREKFLACGIQPDDFRLRRTWQG
                     LDELAERPIDLETFRQLVSPELLMVARVLNKRLIVPEWQDFRQDIQYIYEQVASDRSG
                     ANADYIPILRDADPERWGVAVCTVDGQRLAIGDVDVYHSVQSVSKPLTYAYALAREGE
                     EFTHQFVGVEPSGRPFNALDLLPDMRPYNPCVNAGAIMVAGVVASGAPDLDAREITTQ
                     LMELWAGLCGRCAPVRFSEETMLSERASAYNNFAIAYLLQGRRGLPRDVDLHKMLDVY
                     FSCCSMEMTARMLSVAAATLANGGVCPINGRQVLSTDIVKRTISVMQAAGMYDNAGVF
                     TLEVGLPAKSGVAGSVMVVVPNLLGFATFSPRLDSYGNSVRGVSFCRQLVERYSVHMY
                     DNLSGGRSGCKRDPRVSRQQRKQRDLSDLRWGIANGDHTALRVRDMILHCMVDISLAD
                     GELQAAELAIMESVHSELIGEPALSGALEALARSRADTAAAWRDGSQNGGDVGPARSP
                     FDRLLVELGEQSSLIDDQAREIILETAFQVACADGIIEMEEQEKLRAVAGALGIHEAV
                     LELEINAFRLSQSAQPV"
     gene            complement(3002..3676)
                     /locus_tag="AC16VR_07495"
     CDS             complement(3002..3676)
                     /locus_tag="AC16VR_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011038.1"
                     /translation="MKRTMFALGAALLLSGSLAQAAALNVTIWQAIPGKSAAMMESAM
                     AAKAIQEKLGASVTIALDNTGRLHYGVGGFENWGAWAKWVEKLQDSEDWAAWQQRSGA
                     DPAAIQEENFILNTVPGTDSPVGRDDLGSVYQVFIWDPSGTNVAPLVERALQAAAIHA
                     KAGIAVGVSVDQHLRMHYVMNFKDWADWAKLQDNPIEEFEQFMLRNAANPVGDLVEVY
                     TVNRLP"
     gene            3891..>5441
                     /locus_tag="AC16VR_07500"
     CDS             3891..>5441
                     /locus_tag="AC16VR_07500"
                     /inference="COORDINATES: protein motif:HMM:NF013199.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR6011039.1"
                     /translation="MTSRWWLLAGGILMLLLVACGQRSQPDAPADAAAEPAGEARGGD
                     FDMADTSSVGGRVYLESCATCHEGGVDRAPQRAMLLLMSPESIHRALTKGVMRPQAAA
                     LSEADKVAVAEFLSRSTMGDTQALAAPPGCAGESAGFDYAEPPVFSGWGLDPASTHAI
                     PAETAGLTPANVGKLKLKWAFGFPGALRARSAPALAGGAIFVGSHGGTVYALDRETGC
                     ARWTFDASAEVRTGIVISPWAAGDTSAEPLAFFGDLIGNLYALNAVTGGLVWRARPDE
                     HPNTTLTGTPTLHEGTLYVPVSSLEVVPAREPDYECCSFRGSVVAYDARTGTEKWQTF
                     TVAEEPRPQGVNAAGTQNFGPSGAPIWNSPAIDVRRGQLTVGTGENYSSPADGGSDAI
                     FAMDLVTGKVNWVFQATAGDAWNVACGSGDRTNCPVEDGPDFDFGAGTLLALDVAGRD
                     LVIAGQKSGVVHALDADTGELLWQTKVGRGGVHAGVYFGMAAHGERLFVPISDTDDGR
                     EYAEPPRPG"
BASE COUNT          892 a         1769 c         1814 g          966 t
ORIGIN      
        1 accccatcgg caccctctcg ggcgggcagc gccaggccct cacgctgctc atggcgacct
       61 ggcttaagcc ggacctgctt cttctggacg agcatacggc ggcgttggac cccaagagcg
      121 cggctcaggt gatccgcctc acccacgaga tcatcgcgcg cgaacggatc accacgctga
      181 tggtgaccca ctccatgcag caggcggtgg atctgggcga ccggatcgtg atgatgcacc
      241 agggccaggt gctgcacgac atctccgggg ccgcccggga gcagatcaga gccgaggacc
      301 tgctgagccg tttcgaggac cttcgccgca aggagcagct cgagcggtcg agggagggac
      361 tcctcctcga ggcacagggc atcgaggagc tgatggaagt cttcaaggcg gtcatttacg
      421 tggacgagcg tgtggacgag cgtgaggtgg agctcctccg ggaattcgcc gagcggtgga
      481 acgttcccct gccggacctc acttctcccc ccgtgacggg cgagggcatc atggcggtgc
      541 gcgccgccgt agcggcatac ctgcgggtct ctccgcccac cgagcaggcc tcggagctgc
      601 tcgacgtgct ccagcacttc gtgcacgcag acgagacggt caccgaggaa gagacgacgg
      661 tcatggacga ggtgggtgcg atggtcatcc gctacgtcac cagctcgagc gaccagggca
      721 ggcatcaggt ggtcatcgtg cctcaaaacg aagcgcaggt gggggaggcg cgcgcgttgg
      781 tcccggaagc ggagcccacc ttcatgcggg gcggcaaggt tatctccgtg gggcgtttct
      841 tctcggcgaa gtacgccgac atggtgtgcg aaggctacgt ggccaaaggc ttcttcacca
      901 cgcgcatcga gctctgagcc ccggcccctg ggcaaatcca aggggtgccg gcaggccgag
      961 gaattcggaa agctgaccac ctcccgaacg cacgtaaatg ctgctcccgg tcgcgatttc
     1021 agaatcgaag tgaggctcgc gctgaatggt gcctgcgcgc gccgtgcaaa gccagattac
     1081 ctgctggatt cagccttgga atcctgacac cattggcagc ctgagctgga aggtgtttgc
     1141 gccaactcag aaaagggcct ttactgcctt tctcagatgt gaggcgcttt catggatcgt
     1201 ctcgccgaat tgtttgcccg cgttgcagac tcgggggtct gcacggcggc aacgctgcga
     1261 gaaaaattcc tggcctgcgg gatccagccg gacgatttcc gtctgcgccg gacctggcag
     1321 ggcctggacg aactggcgga gcgtccgatt gatctcgaaa ccttccggca gctggtcagc
     1381 cccgagctgc ttatggtggc gcgtgtactg aataagcggc tgatcgtgcc tgaatggcag
     1441 gacttccggc aggacattca gtacatctac gaacaggtgg ccagcgaccg cagcggtgcc
     1501 aatgccgact acatcccgat cctgcgggat gccgatccgg aacgctgggg cgtggcggtc
     1561 tgcacggtcg acggtcagcg cctggcgatc ggtgatgtgg acgtctacca ctccgtccag
     1621 tccgtctcca agccgctcac ctacgcctac gcgctggccc gggagggaga ggagttcacc
     1681 caccagttcg tcggcgtcga accgtccgga aggcccttca atgccctgga tctcttgccg
     1741 gacatgcggc cttacaaccc ctgcgtcaat gctggcgcga tcatggtggc tggcgtggtg
     1801 gcgtccggcg cgccggatct ggatgcccgg gagatcacca cgcaactgat ggagctctgg
     1861 gccggcctct gtggccggtg cgcgccggta cgcttcagcg aagagaccat gctttccgag
     1921 cgcgccagcg cttacaacaa tttcgccatc gcctatttgc tgcagggccg tcggggcctg
     1981 ccccgcgatg tagatctgca caagatgctg gatgtctact tttcttgctg cagcatggag
     2041 atgaccgcac gcatgctgtc cgtagcggcg gctacgctgg cgaacggggg cgtatgtccc
     2101 atcaacggac gccaggtgct ctccaccgac atcgtcaaga ggacgatctc ggtgatgcag
     2161 gcggcaggca tgtacgacaa tgccggcgtg ttcacgttgg aggtgggcct gccggccaaa
     2221 tccggcgtcg ccggatctgt catggtcgtg gtgcccaacc tgctgggatt cgccaccttc
     2281 tcaccgcggt tggattccta cgggaattcg gtgcggggcg tcagtttctg ccggcagctg
     2341 gtcgaacggt acagtgtcca catgtacgac aatctgtccg gcggccgttc gggctgcaag
     2401 cgtgatccgc gcgtgtccag acaacagcgc aagcagcggg atctctccga tctgcgctgg
     2461 ggcatcgcca atggtgatca caccgccctg cgggtgcgtg acatgatcct gcactgcatg
     2521 gtggatatca gcctggcgga cggcgagctg caggcagccg aactggccat catggagtcc
     2581 gttcatagcg aactgatcgg tgagccagcg ctgtcgggtg ccttggaagc gcttgcccgt
     2641 tcgcgggcag acacagctgc ggcctggcgc gatggttcgc agaacggcgg tgatgtcggc
     2701 ccggcgcgtt cgccgttcga tcggctgctc gtcgagctcg gcgagcaatc cagcctgatc
     2761 gatgaccagg cgcgcgagat cattctcgaa accgcttttc aggtggcctg cgccgacggc
     2821 atcatcgaaa tggaagagca ggagaagcta cgagccgtcg ccggcgccct gggcatccac
     2881 gaagcggtgc tggaactgga gatcaacgcg ttccggctct cccagagcgc tcagcccgtt
     2941 tgacgccggg cggatggccc gctggatgtc cgcccgacgg gcggacatcc gagcgaaccg
     3001 actacggcag gcgattgacc gtgtacacct cgaccaggtc gccgaccgga ttggccgcgt
     3061 tgcggagcat gaactgctcg aactcctcga tggggttgtc ctgcagcttg gcccagtctg
     3121 cccagtcctt gaagttcatc acgtagtgca tccgcaggtg ctgatcgacg ctgacgccca
     3181 ccgcgatgcc ggcctttgca tgaatcgcgg ccgcctgaag tgcccgctca accagcggcg
     3241 cgacgttcgt gccggacggg tcccagatga atacctggta tacgctcccc agatcgtccc
     3301 ggccgaccgg actgtcggtg ccgggcaccg tgttgaggat gaagttctct tcctgtatgg
     3361 cggcaggatc tgcgcccgat ctctgctgcc aggctgccca gtcctcactg tcctggagct
     3421 tttcgaccca cttcgcccag gcgccccagt tctcgaagcc gcccactccg tagtgcaggc
     3481 ggccggtgtt gtccagcgcg atcgtgacgc tggcccccag cttctcctga atggccttcg
     3541 cagccatggc cgattccatc atggctgcag acttcccggg aatcgcctgc cagatcgtca
     3601 cgttcagggc ggctgcctga gcgagcgaac cggacagcaa cagcgccgcg ccgagcgcaa
     3661 acatcgtgcg tttcattcgt tttgtccttt tggtcgtttg actagccggt caggtaccgg
     3721 cacaccgagg tacgtcgaac ttctccccgc ccagaatcta gcacactcgc ccgccactca
     3781 gcgcgtcgtt tcgttctcgc catgttcccg gtctcgctct aggatacccg ggtctatcca
     3841 tggcgccctg gtgcggctcg ctggccgcgc cctcatccct gggaggaagc gtgacttcaa
     3901 gatggtggct gctggccggc gggatcctga tgctgctgct cgtggcctgc ggacagcgtt
     3961 cgcaaccgga cgccccggcc gatgccgccg cggagccggc cggcgaggcg cgcggcggcg
     4021 acttcgacat ggccgatacc tccagcgtgg gcgggcgggt ttacctcgag tcgtgcgcga
     4081 cgtgtcacga agggggcgtc gaccgggcgc cgcagcgggc catgctgctg ctcatgtcgc
     4141 ccgaatcgat ccaccgcgct ctgaccaagg gcgtgatgcg ccctcaggca gccgccctgt
     4201 ccgaggcgga taaggttgcc gttgccgagt ttctctcgcg cagcaccatg ggtgacaccc
     4261 aggccctggc agcgccacca ggctgcgcgg gcgagagcgc cgggttcgac tacgccgagc
     4321 cgcccgtctt ctcgggttgg ggactggatc cggcaagcac ccacgccatc ccggcggaaa
     4381 ccgccgggct gacgccagcc aacgtcggta agttgaagct caagtgggca ttcggctttc
     4441 cgggcgccct gcgtgcgcgc tcggcgccgg cgctggctgg cggtgcgatc ttcgtgggca
     4501 gccatggcgg caccgtctac gcgctcgacc gagagactgg ctgcgcgcgc tggacgttcg
     4561 atgcctccgc ggaagttcgc accggcatcg tcatcagccc ctgggccgcc ggagatacgt
     4621 cggccgagcc gctggccttc ttcggtgatc tcatcggcaa cctctatgcg ctgaacgcgg
     4681 tgaccggcgg cctggtctgg cgcgcgcggc ccgacgaaca cccgaacacc accctgaccg
     4741 gcacgcccac cctgcacgaa ggaacgcttt atgtgcccgt ctcctcgctg gaggtggtgc
     4801 cggcccggga gccggactac gagtgctgct ccttccgcgg gtccgtggtg gcctacgacg
     4861 cccggaccgg gaccgagaag tggcagacgt tcaccgtggc cgaggagccc aggcctcagg
     4921 gcgtcaacgc ggcgggaact cagaacttcg gcccctccgg cgcgcccatc tggaacagcc
     4981 cggccatcga tgtgcggcgc ggacagctca cggtgggcac cggcgagaac tactcgtctc
     5041 ccgccgatgg cggcagcgac gcgatcttcg ccatggacct tgtgaccggg aaggtgaact
     5101 gggtctttca ggccaccgcc ggggacgcct ggaacgtggc ctgcgggtcg ggggaccgaa
     5161 cgaactgccc ggtcgaggac gggcccgact tcgactttgg cgcaggcacc ctgctggccc
     5221 tggacgtcgc cggtcgcgac ctggtcatag ccggtcagaa gtcgggcgtc gtccatgcgc
     5281 tcgatgcgga taccggcgag ctgctctggc agacgaaggt gggccggggc ggcgtgcacg
     5341 cgggcgtcta cttcggcatg gccgcgcacg gggagcgtct gttcgtgccg atcagcgata
     5401 ccgatgacgg ccgcgagtat gcggagcccc cgcggccggg g
//
LOCUS       DBNHQR010000338         2683 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_95232, whole genome shotgun sequence.
ACCESSION   DBNHQR010000338 DBNHQR010000000
VERSION     DBNHQR010000338.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_95232"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..1429
                     /locus_tag="AC16VR_07505"
     CDS             236..1429
                     /locus_tag="AC16VR_07505"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR6011040.1"
                     /translation="MAAMLDGAVHPDFADVAAALIRQIPRGRDGGSAVCVYHRGRCVV
                     DVWGGIRDHHGNPWEHDTTAPSFSTTKGVIATLLHILVDQGKAGYDDPIAHHWPEFGA
                     RNKENITIRQALCHEAGLYRVADMIRRPQEMLDWGHMKRVIADAEPAHEPGSSHGYHA
                     LTYGWLIGGVIEAVAGRPLQAVLQDELVDPLGLDGMFIGMPHNELHRRAELIHGRVRP
                     PRATEPWQETLLEWVDAALGQLGVELAEFSAAMDVFSEDFDWNADETAQAIIPAANGQ
                     FTARSLARMYAMIAQGGELDGVRVLSEGRVREITEVRSRTRDRVLFIPMHWRMGYHRA
                     FAFGVSAPHAFGHYGYGGSGAFCDPSRELAVALILNSGAGTPMGGRYMPLVARAAIRA
                     ADRCR"
     gene            1426..2268
                     /locus_tag="AC16VR_07510"
     CDS             1426..2268
                     /locus_tag="AC16VR_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010448255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacetylase"
                     /protein_id="HIR6011041.1"
                     /translation="MSRQASPAGVDEARLEAFLARHRHLLVITGAGCSTPSGIGAYRD
                     EAGEWLRAEPVQMRDFVASEAARRRYWARSMHGWRAFRDAAPNAAHQALADLERRGVV
                     RGLITQNVDGLHQRAGHDDVIELHGSLRRVVCLECGVSREREEVQRWLEARNGFVLDA
                     SASLAPDGDADLVIDDLSAFEVPRCADCGGLLKPDVVFYGDSVPRDRVDACYAGVAQA
                     DAVLVVGSSLMVFSSFRFVRRAVDLGKPVAAVNRGRTRADAWLEFKVDEDCAAVLERI
                     AANL"
     gene            2490..>2683
                     /locus_tag="AC16VR_07515"
     CDS             2490..>2683
                     /locus_tag="AC16VR_07515"
                     /inference="COORDINATES: protein motif:HMM:NF024803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR6011042.1"
                     /translation="MGGYTTREVSDLIGLKPDQVRHYVRRSLLRPARGDRGEYRFNFQ
                     DVVLLRSAKGLLDADVSSRKA"
BASE COUNT          410 a          876 c          930 g          467 t
ORIGIN      
        1 agaggctgcc ggcgccgggc cgggcgcggc cgccggccgt gatcagctcg tcccacagcc
       61 cttcggccga gtactcttgc cacttgatgc tcatgctcga attcgatcgc gcgccgccag
      121 gtttgcccgc gggtcattct acgggacatg gcgcttgtga tggcagcctt ctcgatgctg
      181 gtgcgcattg cttgcgggtt ccataaaatg tccgccccct aatcccagga ctctgatggc
      241 ggccatgctc gacggcgctg ttcatcccga cttcgcggac gttgccgccg ccctcatccg
      301 gcagataccc agagggcgcg atgggggctc ggcggtgtgt gtctatcatc gagggcgctg
      361 cgttgtcgac gtctgggggg gcatccgcga tcatcacggt aatccctggg aacacgatac
      421 gactgcgcct tcgttctcta cgaccaaggg ggtcatcgcc acgctgctgc acatcctggt
      481 ggatcaggga aaggcgggat acgacgaccc cattgcgcac cattggcccg agttcggcgc
      541 ccgcaacaag gagaacatca ccatccgcca ggcgctctgc cacgaggccg ggctctaccg
      601 cgtggcggac atgattcggc ggcctcagga gatgctggac tgggggcaca tgaaaagggt
      661 cattgccgac gccgagcccg cccacgagcc tggcagctcc cacggttatc acgcgcttac
      721 ctacggctgg ctcatcggcg gcgtcatcga ggcggtggcc gggcgtcccc tgcaggcggt
      781 tcttcaggac gagctggtcg accccctggg cctcgacggc atgttcatcg gcatgcccca
      841 caacgagctc caccggcgtg ccgagctgat ccacgggcgc gtccggccgc ctagagccac
      901 cgagccgtgg caggaaaccc tgctggaatg ggtcgacgcg gcgttggggc agctgggggt
      961 ggagctggcg gaattcagcg cggccatgga cgttttttcc gaagacttcg actggaacgc
     1021 cgacgagacg gcgcaggcca tcatcccggc ggcaaacggt cagttcacgg cccgctcgct
     1081 tgcgcgcatg tatgccatga tcgcccaggg cggcgagctg gacggggtca gggtcctgtc
     1141 cgaggggagg gtccgcgaga tcaccgaggt ccgcagccgt acccgtgatc gcgtgctctt
     1201 catccccatg cactggcgca tgggctatca ccgggcattc gcgttcgggg tcagcgcgcc
     1261 ccacgcgttc ggacattacg ggtatggcgg gagcggagcg ttctgcgatc cgagccggga
     1321 gcttgcggtg gcgctcatcc tgaacagcgg agcgggtacc ccaatgggcg gccgctacat
     1381 gccgctggtt gcgcgcgccg cgattcgcgc cgccgatcgg tgccggtgag ccggcaagcc
     1441 agcccggccg gcgtcgacga agcccggctg gaagcattcc tcgcccgtca tcggcatctg
     1501 ctggtcatta ccggcgcggg ttgcagcacg ccgtcgggaa tcggggccta tcgggacgag
     1561 gcgggagaat ggctgcgtgc cgagccggtt cagatgcgtg acttcgttgc cagcgaggca
     1621 gcgcgtcggc gctactgggc ccggagcatg cacggctggc gggcgtttcg cgatgccgca
     1681 cccaacgccg ctcaccaggc gctggcagac ctggaaaggc gcggcgtcgt gcggggactg
     1741 atcacccaga acgtcgacgg gctgcaccag cgggcgggac atgacgacgt catcgagctg
     1801 cacggcagcc tgcggcgggt cgtctgcctc gaatgcgggg tgagccgcga gcgcgaggag
     1861 gtgcagcgat ggctggaagc ccgcaacggg ttcgtcctcg atgccagtgc cagccttgcg
     1921 cccgacggcg atgcggacct cgtcatcgac gatctctccg ccttcgaagt gccacgctgt
     1981 gcagactgcg gcggcttgct caagccggac gtggtgttct acggagactc cgtgccccgc
     2041 gaccgggtgg atgcgtgcta cgcgggcgtt gcccaggcag atgccgttct cgtcgtgggc
     2101 tcgtccctga tggtcttttc gagcttccgt ttcgtgcgtc gcgcggtgga cctgggaaag
     2161 ccggtggccg cggtgaaccg ggggcgaacc cgggcggatg catggctcga gttcaaggtc
     2221 gacgaagact gtgctgctgt gctggaacga atcgccgcca atctctaggt gggccttgtg
     2281 gcgggcttcc ggcccggctc gcagcagggc tcgcagacgc gcctccaccc gggcctccgc
     2341 ccggggactt tgcccggccc gtgttgaggg ctcaccaccc ctacgctata gtgccggcgt
     2401 tccctgccgc cggcggtctc gaagaggcgg gaagccgtgg ctagacacct cggtcaggca
     2461 ccaatgaagg aaccgcatct gccaatctga tgggcgggta taccactcgg gaagtctctg
     2521 atctcatcgg gcttaagccc gatcaggtcc gtcactacgt tcggcgcagc ctcctgcggc
     2581 cggctcgtgg agaccgcggc gagtaccgct tcaatttcca ggacgtcgtg cttctccgca
     2641 gcgccaaggg tctgctcgac gcggacgttt cctcgcgcaa ggc
//
LOCUS       DBNHQR010000339         1754 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1615633, whole genome shotgun sequence.
ACCESSION   DBNHQR010000339 DBNHQR010000000
VERSION     DBNHQR010000339.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1615633"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..125
                     /locus_tag="AC16VR_07520"
     CDS             <1..125
                     /locus_tag="AC16VR_07520"
                     /inference="COORDINATES: protein motif:HMM:NF036590.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminopeptidase N C-terminal domain-containing
                     protein"
                     /protein_id="HIR6011043.1"
                     /translation="RRYDTHRQALMTAALRRIEDQEGLSKDVFEVVTKTLATED"
     gene            complement(141..>1754)
                     /locus_tag="AC16VR_07525"
     CDS             complement(141..>1754)
                     /locus_tag="AC16VR_07525"
                     /inference="COORDINATES: protein motif:HMM:NF024973.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HIR6011044.1"
                     /translation="LFAPWAWSAIQTSVEPRLLDEMDTLRLTLRSTGQSRADTLDLSP
                     LDEDFEVLGTNTQSQYRSVNGRIDSWVEHQIDLRPRRTGELLVPAISIGGEQSEAIRI
                     VVRPMDPGVRRAIDRMVFFETELSSNPVYVQGETVFTRRLYYSSGVQIYSDLPGAPEV
                     PDAVVIPVGETRSSSTLVGGQRYGIVEQQFAIFPERSGAVVIPETSITSSVRMQTGGR
                     YRRSGIRVSTEPIELEVLPIPPAYPQHEPWLPATDVQLSEAWSPSTTEITVGDPVERR
                     FTLVATGNIGSAIPPIDAKFPEGRLKSYPQSPEILDDTRGSSVVGTRQEVYSLIAVAG
                     GEVAFPPVEVTWWDTGTERVRKSRLDGRMVRIAGGRPALPQKPLAEDPVSDGSGVGAE
                     PAAAAGSDLEPGLGLNPLHAAAILFAFLLLAGFWALSGRFRSSAPDAGPTSRQAWASL
                     RRSCQNQEPQVLRAALLQYLAARFGASGLEQLLAHPQGREVWQQLNESLFSPQPGPRV
                     TGQDVIKAAALTRGRRKPSGPEALPELYA"
BASE COUNT          294 a          551 c          617 g          292 t
ORIGIN      
        1 ggcgacgcta cgacactcac cgtcaggcgc tgatgactgc ggcattgcgc aggatcgagg
       61 atcaggaagg gctgtccaag gacgtattcg aggtggtcac caagacgctg gcgaccgaag
      121 actgacctgc cccgggccgg tcaggcgtac agctcgggaa gcgcttcggg cccggagggt
      181 ttgcgccgtc cccgggtcag ggcggcggct ttgatgacgt cctggccggt gactcgcggg
      241 ccaggttgcg gcgagaagag agattcgttt agctgctgcc agacttcccg cccctggggg
      301 tgcgccagca gctgctcaag tccggaggct ccgaagcgcg cggcgaggta ttgcagcagc
      361 gcggcgcgca ggacctgggg ttcctggttc tggcagctgc ggcgcagcga cgcccaggcc
      421 tgcctcgagg tcgggcccgc gtcgggcgcg ctggatcgga aacgtccgga gagagcccaa
      481 aacccggcca gcagcaggaa cgcgaagagg atggcggctg cgtgaagcgg gttcaaccca
      541 aggccgggct cgagatccga gccggctgct gccgcgggct cggcgccgac gcctgaccca
      601 tcggaaacag ggtcttcagc gagaggtttc tggggcaagg cgggtcttcc gccggcaatg
      661 cgcaccatgc ggccatccag ccggctcttc ctcacgcgtt cggtcccggt atcccaccac
      721 gttacctcta ccggaggaaa tgccacctcg ccgccggcga cggcaatcag cgagtaaacc
      781 tcctggcggg tgccgacgac gctgctgccg cgggtgtcgt ccaggatctc cggagattga
      841 ggataggatt tcaggcggcc ttcagggaac ttcgcgtcga tcggcgggat cgcggagccg
      901 atgttgcccg tggcaaccag ggtgaagcgg cgttccaccg ggtcgccgac ggtaatctcg
      961 gtggtgctgg gcgaccaggc ttcggaaagc tggacgtcgg ttgcgggcag ccacggctcg
     1021 tgctgggggt aggccggcgg gatgggaagc acttccagct cgatgggttc ggtgctgacc
     1081 cggataccgg agcggcggta gcgcccgccg gtctgcatgc ggacgctgga cgtgatggac
     1141 gtctccggga tgacgaccgc gccagagcgc tcgggaaata tggcgaattg ctgctctacg
     1201 atgccgtagc gctgcccgcc cacgagagtg ctgctcgatc gcgtctcgcc caccggtatg
     1261 accaccgcgt ccggcacttc cggggccccg ggcaggtcgc tgtatatctg cacgccgctc
     1321 gagtagtaga gcctgcgcgt gaacacggtc tcgccctgaa cgtataccgg gttgctggac
     1381 agctcggtct cgaagaagac catgcgatcg atggcacgcc tcacgcccgg gtccatggga
     1441 cgcaccacga ttcggatcgc ctcgctctgc tcgccgccga tggagatcgc cggcaccagc
     1501 aactcgcccg ttcgacgtgg ccgtagatcg atctggtgct cgacccagct gtctatgcgt
     1561 ccgtttacgg agcggtactg gctctgggta ttggtgccga gcacctcgaa atcctcatcg
     1621 agcggcgaca ggtccaatgt gtccgcccgg ctctggcctg tggagcgcaa agtcagccga
     1681 agggtatcca tctcgtcgag cagccgaggc tccacggaag tctggatggc tgaccatgcc
     1741 caaggtgcga acag
//
LOCUS       DBNHQR010000340         1898 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_982407, whole genome shotgun sequence.
ACCESSION   DBNHQR010000340 DBNHQR010000000
VERSION     DBNHQR010000340.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_982407"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /gene="ltrA"
                     /locus_tag="AC16VR_07530"
     CDS             complement(<1..989)
                     /gene="ltrA"
                     /locus_tag="AC16VR_07530"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HIR6011045.1"
                     /translation="MTQAKSFSIPKRDVWEAFKRVKANRGAAGVDGQSIEAFEARVTA
                     NLYKLWNRLSSGSYMPKPVRRVEIPKAGGGKRPLGIPTVADRTAQELVRRFLEPILEP
                     GFHPDSYGYRPGRSAIDAVRTARQRCWRYDWVLDIDIKGFFDNIDHGLLLRALRRHTD
                     CAWVLLYVERWLKAPVMLEDGDLVERDRGTPQGGVISPLLSNLFLHYAFDFWMQREMP
                     GVPFERYADDIICHCRSEGQARALWQRLESRLRECGLELHPQKTKIVYCKDTNRKEEF
                     DCVSFDFLGYRFQPRLAKWRGGIFGVSFLPAAKPTALATIRRQVRNWGLQRRS"
     gene            complement(1530..1898)
                     /locus_tag="AC16VR_07535"
                     /pseudo
     CDS             complement(1530..1898)
                     /locus_tag="AC16VR_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
BASE COUNT          339 a          684 c          519 g          356 t
ORIGIN      
        1 tcgctgcgcc gctgcagtcc ccagttgcgc acttgccgtc gtatggtcgc aagtgccgtg
       61 ggtttagcag cgggaaggaa cgatacaccg aagatcccgc cacgccattt cgccaatcgg
      121 ggctggaaac gatagcccag aaagtcgaag ctgacgcagt cgaactcttc cttgcgattg
      181 gtatccttgc aataaacgat tttcgtcttt tgcggatgta gttccagacc gcattcccgc
      241 agacgacttt ccagccgctg ccagagcgca cgtgcctgcc cctcgctccg gcaatggcag
      301 atgatgtcgt ccgcataccg ctcaaacggc acacccggca tctcccgctg catccagaag
      361 tcgaaggcgt aatgcaggaa caggttggac agcagaggcg agatcacccc accctgcggc
      421 gttcccctgt cccgttcaac aaggtcgcca tcttcgagca tcaccggcgc cttcagccaa
      481 cgctcaacgt agagcagaac ccacgcacaa tcggtatgcc gacgcagcgc gcggagcaaa
      541 agcccatggt cgatgttgtc aaagaacccc ttgatgtcga tatcgagcac ccagtcataa
      601 cgccagcatc gctgacgcgc cgtccggacc gcatcgattg ccgagcgccc cggccgatac
      661 ccgtacgagt ccggatggaa ccccggctca aggatcggct cgagaaagcg gcggacaagc
      721 tcctgtgccg tgcggtccgc gaccgtgggt atccccaacg gcctcttccc gcccccggct
      781 ttcggtatct ccaccctgcg caccggcttt ggcatatagc tgcctgacga cagccggttc
      841 caaagcttgt agaggttggc ggtaacccgc gcctcgaagg cttcaatcga ctgcccgtct
      901 actccagccg ccccgcgatt ggccttcaca cgcttgaagg cctcccacac atcccgtttc
      961 gggatcgaga acgactttgc ctgcgtcatg cggctcctcc cgtcaccggt tggccgcccg
     1021 cacaggctga acgtcacagc cccttcggtc cagcgccatt acagcgcctt caaccctact
     1081 acggactgtt ctgcccctgt gccccgcatc ggtactccgg gcctcatggg ggttgcccac
     1141 tcggccttct cccttggcat cgggacgaca ggttctcctg ttccacgcaa gagcctgatc
     1201 caaattcacg ccgccttcaa gccggacgcc gcgcgggccg gtcttcaggg ctccgcccgc
     1261 acttatcccg gagtgaccac cacccccggt tctgacgtca acgatacgat ttcgacaggt
     1321 catcagcggt tcgctttcgc tcgtctcttt ggatcatacc tgacgggatc attgtcccgc
     1381 cgtttccgtg acgctcacca ccatcgctct taacgacagc agctcacggt ggtttgggat
     1441 cggctcctga tggccgaacc cgaggggcct accctcatct cctgcgcagc ttcgcacccc
     1501 ctttctttga cgtgcttcca ggacacacca tgatcaccga acttcgagac aatcacctgg
     1561 gcgatcaggg cttcggtcgg cagcccgccg ggcaccacgt gttcgggggc atgggcctgg
     1621 accacggtgc ccgagcagcg ccggcaggca tatcggggac gccgcgtgac cagcacacgg
     1681 aactgcgccg ggatcacgtc gaggcgctcg gagacgtctt caccgatctt cgccatctca
     1741 ccgcagccgc aggggcacat cgtgctctcc ggctcgatca ccctctcgac ccgcggcaga
     1801 tgcgccggca gccgaccgcg gttacccgcc cgcccgcgcg gcgcaccggc cggcttaccc
     1861 ctgatggcgg cctcggcttt ctcctgcgcc gcgtcgag
//
LOCUS       DBNHQR010000341         2045 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_474865, whole genome shotgun sequence.
ACCESSION   DBNHQR010000341 DBNHQR010000000
VERSION     DBNHQR010000341.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_474865"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1092
                     /gene="aceK"
                     /locus_tag="AC16VR_07540"
     CDS             <1..1092
                     /gene="aceK"
                     /locus_tag="AC16VR_07540"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010208603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HIR6011046.1"
                     /translation="GAQLWPFALALLLDDDGRLYVDTLICDVDELSVMFSFTRAYFMV
                     LTDYPHALVDFLQVLLPNKKRTELYASMGLHKHGKTVFYRDFLDHLSRTDDQFVIAPG
                     TPGMVMTVFTLPSLQTVFKVIKDEFAPQKNITAQQVRDKYYVVKSHDRVGRMADTQEF
                     QNLTFPIDRFDPELVEELRRVAPSSIEISGNEILIRHLYTERLMTPLNLFIDTADEGA
                     LREALEEYGNAIKQLAAANIFPGDMLLKNFGVTRHGRVVFYDYDEICYLTDVNFRDIP
                     EPRSPEDEMAAEPWYAVGPHDVFPEEFRRFLFGRRKIKQMFEEMHGELFEPSYWRGLQ
                     AAINDGQVMDVFPYRRKRRFGEHGAASRA"
     gene            1109..1780
                     /locus_tag="AC16VR_07545"
     CDS             1109..1780
                     /locus_tag="AC16VR_07545"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6011047.1"
                     /translation="MEQLDTRARERLEKADALIFDFDGTLAPNLDLPDMRRQVVDLTL
                     GRGVPGEIFEHLYIVEIIEAAASWLRRRDRTSAADYHEAAHRLITEFELEAARNTEPF
                     PATRTTLATLRRKGKRLGVVTRNCSTAVRTVFSDIDVYIDAVLARDDVDFLKPDARHV
                     RQALSVLDGTPERAAMIGDGRLDMHVGRDLGMTCVGVLTGSADADALLEAGAHIIVGA
                     LEELL"
BASE COUNT          377 a          675 c          632 g          361 t
ORIGIN      
        1 ggagcacaac tgtggccatt tgcgctcgcc ctgctcctgg acgacgacgg gcgcctctac
       61 gtggacacgc ttatctgcga tgtggacgag ctgtccgtca tgttcagctt cacccgcgcc
      121 tacttcatgg tcctgacgga ctaccctcac gcgctggtgg acttcctgca ggtccttctg
      181 cccaacaaga agcgcaccga gctctatgcc tccatgggac ttcacaagca cggtaaaacg
      241 gtcttctatc gtgactttct ggatcatctg agccgcacgg acgatcagtt cgtgatcgcg
      301 cccggaacgc cgggcatggt gatgaccgtg ttcacgttgc cctccctgca gaccgtgttc
      361 aaggtgatca aggacgagtt cgcgccccag aagaacatca ccgcccagca ggtccgggac
      421 aagtactacg tggtgaagtc ccacgatcgg gtcggccgca tggccgatac ccaggagttt
      481 cagaacctca ccttccccat cgaccggttc gatccggagc tggtggagga actcaggcgc
      541 gtagcaccct ccagcatcga gatttccgga aacgagattc tgatccgcca cctgtacaca
      601 gagcggctga tgaccccttt gaacctgttc atcgacacgg ccgatgaagg ggccctgcgc
      661 gaagcgctgg aagagtacgg caacgccatc aagcagctgg ctgctgccaa catctttccc
      721 ggcgacatgc tgctcaagaa cttcggcgtc acccggcacg gccgcgtggt gttctacgac
      781 tacgacgaga tctgctacct caccgacgtc aatttccggg acattcccga gccacgctcg
      841 cccgaagacg agatggcggc cgagccatgg tatgcagtgg ggccccacga cgtgtttccc
      901 gaggagtttc gccgtttcct gttcggccga cggaagatca agcagatgtt cgaggaaatg
      961 cacggcgagc tgttcgagcc ttcctactgg cgcggcctgc aggcagccat caacgacggt
     1021 caggtgatgg acgtctttcc gtatcggcgc aagaggcgtt tcggcgagca cggcgccgcg
     1081 agccgggcct gacacccagg ggagaccggt ggagcagctg gacacgaggg cccgcgagcg
     1141 gctggagaag gccgacgccc tgattttcga cttcgacggc accctggctc ccaacctgga
     1201 tcttcccgac atgcgccggc aggtggtgga cctgaccctc ggacgcggcg tgcctgggga
     1261 gatcttcgag catctctaca tcgtggagat catcgaggcg gccgcgtcgt ggctgcggcg
     1321 acgcgatcgc acttcggccg ccgactatca cgaagccgcc catcggctaa tcaccgagtt
     1381 cgagctggag gctgcccgga acaccgagcc gttcccggcc acgcggacca ccctcgctac
     1441 tctgcggcgg aaagggaagc gccttggcgt ggtaacgcgc aactgctcga cggcggtgag
     1501 gaccgtcttt agcgacatcg acgtatacat cgacgccgtc ctggcacgcg acgacgtgga
     1561 ctttctcaaa cccgacgccc ggcacgtgcg ccaggcgctg agcgtcctcg acgggacccc
     1621 tgagcgagcg gccatgatcg gcgacggccg cctcgacatg cacgtcggtc gggatctggg
     1681 catgacgtgc gtgggcgtgc tcaccgggtc cgccgacgcg gacgccctgc ttgaagcagg
     1741 cgcccacatc atcgtgggcg cgttggagga gctgctgtga cgtcgcttcc ggcaccgggc
     1801 gcgacaggct ggccttccgc ggcgactggg ttcagaattg cgggcagtcg cgtcggtcct
     1861 ggaacctgga gaaggagatg catcgagcca cgcaagcacg caatgccatc gcccctctgc
     1921 tggaccagct catcgtccag ctggacgccg agggccgggc ttcccaacgc gcctacttcg
     1981 ctcgaatccg ccgttccctg cttcttgcac gacacgaagc cgacctagcc acgcccattc
     2041 gcgag
//
LOCUS       DBNHQR010000342         2728 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_95381, whole genome shotgun sequence.
ACCESSION   DBNHQR010000342 DBNHQR010000000
VERSION     DBNHQR010000342.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_95381"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..146)
                     /locus_tag="AC16VR_07550"
     CDS             complement(<1..146)
                     /locus_tag="AC16VR_07550"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253700.1"
                     /note="catalyzes the transamination of the branched-chain
                     amino acids to their respective alpha-keto acids; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid aminotransferase"
                     /protein_id="HIR6011048.1"
                     /translation="MTEPTFSDRDGWIWHNGELVPWRDAKVHVLTHTLHYGVGVFEGV
                     RAYQT"
     gene            complement(157..>2728)
                     /gene="glnE"
                     /locus_tag="AC16VR_07555"
     CDS             complement(157..>2728)
                     /gene="glnE"
                     /locus_tag="AC16VR_07555"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HIR6011049.1"
                     /translation="FSAPLASGEVRATLAAVSQGAEDLGALQQSLRQWRNRYQLWVVW
                     RHLLRMAPLEETCATLSMLADTMLDLALERLYQWATDRQGVPMGESSRSPQRLVVLAL
                     GKLGARELNLSSDIDLVFAYPEPGATTSGTTNQQFFLRLGQQLIQALDAVTADGFAFR
                     VDMRLRPFGASGPLVMHFEGMEDYFAAHGRDWERYAWIKARACAGDVASGEALLQSLR
                     PFVYRRYLDFGAVDALRDMKRRLYSERNDPNDVKLGPGGIRDVEFVVQLQQMVWGGRQ
                     PELQEANLLRVLPRLASQGHLSDADAAALAEGYRFLRDTEHSIQAEGDLQTQSLPASE
                     DSRQRLASSRGFADYGAFESALNGHRIAVEAVFRNAVGELPEEDTPGQRLWSSPDDAA
                     LLGELGFARAEAAGKLLTSLMEARDRPSQSAESRARLDRLMPGLLDRVSLTEDPDLSL
                     ARVVPVLRSVLRRSAYLSLLRENPHTLAHFVDLTAKSLWLAEQLARFPAFFDALLDER
                     QLAALPDKAALQAELHQQVEAAPADDFERRLDVLREFKEHHVFNVSLAEVRGTLPLMK
                     ASDYLTFLAEAVLGESLDLAWNENADRFPEFAEPRPFIIVGYGKLGGIELGPGSDLDL
                     VFIHDLPVGASQFLHRMVRRLLHILTVPTYSGTLYEIDMRLRPSGSSGTMVSSLEAFA
                     EYQRSQAWMWEHQALVRARAVAGDPALAQRFEAVRRELLCEPRDRGPLREEVLKMRAR
                     MAEHHGDAEDLKRGSGGIVDIEFMVQYLVLAWAHEHPALAEFSDNVRILETAERLDLL
                     PANMARNLREAYVGLRSEWHRSVLDLPDSERAARTLMAHRSDVRDAWREVFGIDPENE
                     "
BASE COUNT          479 a          921 c          883 g          445 t
ORIGIN      
        1 gtctgatacg cccgcacgcc ttcgaacact cccacgccgt agtgcagcgt gtgagtcagc
       61 acgtggactt tcgcgtcacg ccagggcacc agttccccgt tgtgccagat ccagccgtct
      121 cggtccgaaa atgtcggttc tgtcatgatt ccgcgcttac tcgttttccg ggtcgatacc
      181 aaacacctcg cgccacgcat ccctcacgtc ggacctgtgg gccatcaggg tccgggcagc
      241 ccgctcgctg tccggcaggt caagcaccga ccggtgccac tcagagcgca gacccacgta
      301 agcctcccgg aggttccggg ccatgtttgc gggaagcaga tccagccgct cagccgtctc
      361 tagaatgcgc acgttgtctg agaattcagc gagcgcaggg tgttcgtgcg cccaggccag
      421 gaccaggtat tgcaccataa attcgatatc gacgataccg ccggagcctc gcttgaggtc
      481 ttccgcgtca ccatggtgct cggccatgcg ggctcgcatc ttcagcactt cctcgcgcag
      541 cggcccgcgg tcccggggct cgcacagaag ttcccgtcga accgcctcga agcgctgggc
      601 gagagccgga tcgccagcca ctgcgcgcgc ccgaaccagc gcctgatgct cccacatcca
      661 ggcctgggat cgctggtact cggcgaaagc ctccaggctc gaaaccatgg ttcccgaact
      721 gcccgaaggg cgcaggcgca tgtcgatctc gtaaagcgtc ccggaatagg tgggaacggt
      781 gaggatgtga agcaggcggc ggaccatccg gtgcaggaac tgcgaggcgc cgacgggcag
      841 gtcgtgaatg aagaccaggt ccaggtcgct tcccggcccg agctcgatac cgccgagctt
      901 gccgtaaccg acgatgatga aaggtcgagg ctcggcgaac tcgggaaagc ggtcggcgtt
      961 ttcgttccag gccaggtcca gcgactcgcc gagcacggcc tcggccagaa acgtgaggta
     1021 gtcgctcgcc ttcatcaggg gcagcgttcc acgaacctcc gccagggaga cattgaacac
     1081 gtgatgctcc ttgaactccc tgagcacgtc gagccgacgt tcgaagtcat ccgccggcgc
     1141 cgcctcgacc tgctggtgca gctcggcctg aagggcggcc ttgtccggaa gcgcagcgag
     1201 ctggcgttcg tccagcagcg cgtcgaagaa cgctggaaag cgggcgagct gctcggccaa
     1261 ccacaagctt ttggccgtca ggtcgacgaa gtgagcaagg gtgtgggggt tttcgcggag
     1321 cagcgacaga taggccgacc gccgcagcac ggatcgcaac acgggcacca cgcgggcaag
     1381 cgagagatcc ggatcctccg taaggctcac ccggtcgaga aggccgggca tgagccggtc
     1441 cagccgggct ctgctttcgg cgctctggga gggccgatcg cgcgcttcca tcaggctggt
     1501 cagcagcttg cccgccgcct cggctcgcgc gaaacccaat tcgccgagca gcgcggcgtc
     1561 atccgggctt gaccagaggc gctggccggg cgtgtcttcc tccggcagct cccccaccgc
     1621 gttcctgaag acggcttcaa ccgcgatccg gtgcccgttc agcgcgcttt cgaacgcgcc
     1681 gtagtcggca aagcccctgg agctggcaag ccgctggcgg ctgtcttcgg acgccggcag
     1741 cgactgggtc tgaaggtcgc cctcggcctg gatgctgtgc tcggtgtcac gcaggaatcg
     1801 atagccttcg gccagcgcgg ccgcgtccgc gtcgctcaga tggccctggg aggcgagccg
     1861 cggcaggaca cgcagcaggt tggcttcctg cagctccggc tgcctcccgc cccagaccat
     1921 ctgctgcagc tgaacgacga actccacatc cctgatcccg ccgggcccga gcttgacgtc
     1981 attgggatcg ttacgctcgg agtagagcct gcgcttcatg tcgcgcagcg cgtctactgc
     2041 cccgaaatcg agataccgcc tgtacacgaa gggccgcagg gactgaagca gggcctctcc
     2101 cgacgctacg tcccctgcgc aggctcgcgc cttgatccag gcatagcgct cccagtccct
     2161 gccatgagcg gcgaagtagt cctccatgcc ctcgaagtgc atgacgaggg gcccgctggc
     2221 gccaaatggc cggagcctca tgtcgacgcg aaaggcaaag ccgtccgccg tcaccgcatc
     2281 cagcgcctga atgagctgct gacccaggcg gaggaagaac tgctggttgg tggtcccgct
     2341 cgtcgtggcg ccgggctccg ggtaggcgaa taccagatcg atatccgacg agagattcag
     2401 ctcacgggca ccgagtttcc ccaacgccag caccaccagg cgctggggcg atcggctcga
     2461 ctcccccatc ggcacgcctt gcctgtccgt ggcccactgg taaagacgct ccagggcgag
     2521 atccagcatg gtgtctgcca gcatggagag ggtggcgcag gtttcttcca gcggcgccat
     2581 ccgcaggagg tggcgccaga ccacccagag ctgatagcgg ttgcgccact gccgcaggct
     2641 ctgctgcagg gcgcctaggt cctcggcccc ttgggaaacg gcggcgagcg tcgcgcggac
     2701 ttcccccgac gcgagggggg cggagaag
//
LOCUS       DBNHQR010000343         4440 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_95524, whole genome shotgun sequence.
ACCESSION   DBNHQR010000343 DBNHQR010000000
VERSION     DBNHQR010000343.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_95524"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..1171)
                     /locus_tag="AC16VR_07560"
     CDS             complement(125..1171)
                     /locus_tag="AC16VR_07560"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6011050.1"
                     /translation="MRISMLLSYAGGFKEAVKEVAELEKAGLDLVWVPEAYSFDAPSA
                     MGYLAANTERVTIASGILPIYSRTPSLLAMTAAGIDYLSDGRCMLGLGASGPQVIEGF
                     HGVPYVAPVSRIREIIEICRKVWRRERLQHDGRYYQIPLPQNKGTGLGKPLKLINHPV
                     REEIPIAIAALGAKSVEMTAELADAWLPAFYTADAADAVWGDALRAGAAKRDPGRAPL
                     EVFAGGAVAIGEGLEDLRNLARPQAALYIGGMGARQKNFYNDIFSRSGYAQEAKLIQD
                     LYLSGDKKGAEAAIPESYLANNSLIGPEGFVRDRLQALSESGVTALNVGFMGRTSQER
                     IRSCEQLRKLVDTL"
     gene            1296..2513
                     /locus_tag="AC16VR_07565"
     CDS             1296..2513
                     /locus_tag="AC16VR_07565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HIR6011051.1"
                     /translation="MDREFDVIVWGASGFTGRLVAEYLNERYGLGGSVAWAIAGRNQG
                     KLDAVRQEIGAPGLSTILADGNDEASLAAMAERARVICTTVGPYALYGSRLVAACVSR
                     GTHYCDLTGEVHWMRRMIDNHHLSAQQAGVRIVHTCGFDSIPSDIGVLCVQREMKSRH
                     GEVATEVKSRVRGMRGGFSGGTVASMLNMMEESEMDPGVREVMNDPYGLNPAGERHGL
                     DGPEHSVPEYDPDFGAWTTPFVMAAINTKVVRRSNALSGYAYGRDFRYDEAMLTGFGP
                     WGFPLAAAMSVGSTAFNAAAGVSALRGLLARVLPEPGEGPSAAARERGFFDIEMLGKS
                     RRSGARVKVRIRGDRDPGYGSTSRMLGESAVCLARDDLETPTGVLTPAVAMGEALIGR
                     LGNNAGVTFEVMP"
     gene            2557..3636
                     /locus_tag="AC16VR_07570"
     CDS             2557..3636
                     /locus_tag="AC16VR_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011052.1"
                     /translation="MSETGPAPQERGKSNILLRIATWLITAGCFYLVYGRTAEAAARD
                     GLTVLEYLARFFGEANWLAWLAVMVPYSVFFFVVDAHVTWRVIRWFNAPEIRLGNVLP
                     IRASAYILSLVNEQVGKGAMSLYLYRRYKVPALAAISSMLLLGLMEIYQLLVFSSVGV
                     FLGFEVVQAASTELPLDKILPGVFAVAAIYLPLHIAYFRGHLLKGSTVREKSLVRAFR
                     EARPIHYLLILLFKAPLLIGAIVVYTIALNLFNVDVSFAQMLAFLPVIFLAAALPLPF
                     HAGALLFWTILFPDFPEVGAFSLVMHTFFVLFNALIGVVFLPIANRELFGSEQPGSGT
                     VAHGNDAGDDEHPGQPLDQGGAGPR"
     gene            complement(3526..4053)
                     /locus_tag="AC16VR_07575"
     CDS             complement(3526..4053)
                     /locus_tag="AC16VR_07575"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR6011053.1"
                     /translation="MWFTWANLLTTVRLALAVPCAMAVVSGDWPLAAALFALAVITDL
                     LDGPLARRLNHASPVGGLFDHATDALFVTFALGALATAGYLTWALPCLVAAAFIQYAL
                     DSRALAGKHLRTSYLGRMNGIAYFFAAGIPIVKEGLALPWPSAALIQGLAWVLVVTSI
                     VSMGDRARAWLLRSE"
     gene            4176..>4440
                     /locus_tag="AC16VR_07580"
     CDS             4176..>4440
                     /locus_tag="AC16VR_07580"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HIR6011054.1"
                     /translation="MDRQGLPMIVVTGANGNLGRRLLQSLSGRAPLRAVVRSERAAEQ
                     IRALTLEPEPEICVVDYQDAAAMTGALAGAEYVVHLVGIIKETA"
BASE COUNT          706 a         1416 c         1496 g          822 t
ORIGIN      
        1 tggaggtggc gccccgcgtc ccggtccgca ccgaggtgca ggttttcccg ctcgagcgga
       61 cggcggatgc gctcgatgct ctgcgggaag gccgcgtcga gggaggcgcc gtcgtccagg
      121 tgcgctagag cgtgtccacc agcttgcgca gctgctcgca gctgcgaatc cgctcttgtg
      181 acgttctgcc catgaagccc acgttcaggg cggtcactcc tgattcgctc agcgcttgca
      241 ggcggtctcg aacgaaaccc tcgggcccga tgagcgagtt gttggccagg tagctctcgg
      301 ggatagcggc ctcggcccct ttcttgtccc cggacaggta caggtcctgg atcagcttgg
      361 cctcttgtgc gtacccggag cggctgaaga tgtcgttgta gaaattcttc tgtcgcgcgc
      421 ccatgccgcc gatgtaaagc gctgcctggg ggcgcgccag attccgcagg tcttcgaggc
      481 cctcgccgat ggccaccgcg ccgccggcga acacttccag cggcgccctg cccggatccc
      541 gcttcgcggc tccggcgcga agcgcatcgc cccacaccgc gtccgcggca tcggcggtgt
      601 agaaggcggg aagccaggca tcggcgagct ccgccgtcat ctccacgctc ttggcgccta
      661 gtgcggcgat ggcgatggga atctcttcgc ggaccgggtg gttgatgagc ttcagcggct
      721 tgcccaggcc ggtgcctttg ttctgcggca gcggaatctg gtagtagcgc ccgtcgtgct
      781 gcagacgctc gcggcgccaa accttccggc agatctcgat gatttcccgg attcgggaga
      841 cgggtgccac gtagggcacg ccgtgaaagc cttcgatgac ctgaggccca gacgcgccga
      901 gcccgagcat gcagcggccg tccgagaggt agtctatgcc tgctgccgtc atggccagca
      961 gagacggtgt ccgggagtag atgggcagga tgccggaggc gatggtgacg cgctcggtgt
     1021 ttgccgccag gtagcccatg gcgctgggcg cgtcgaagga gtacgcctcg ggaacccaga
     1081 ccagatcgag accggctttc tccagctcgg cgacttcttt gaccgcttcc ttgaaaccgc
     1141 cggcatagct cagcagcatg gatatacgca tgttcaaccc cttgagaagg tgatggttcg
     1201 ttggtcgccg gcccggccgc ggagtatgct tggcggctgc cgcccctttg ccaccggcgt
     1261 ggcggtcgat gttaacagaa tgaggatcac tgccgttgga tcgggaattt gacgtcatcg
     1321 tctggggggc cagcggtttc accgggcgcc tcgtagccga gtacctcaac gagcgctacg
     1381 gattgggcgg aagcgtcgcc tgggcgatcg ccgggcgaaa tcagggcaag ctcgatgcgg
     1441 tgcgtcagga gatcggcgcg ccgggactgt ccacgatcct ggccgacggc aacgacgagg
     1501 catcgctcgc cgccatggcc gagcgcgccc gcgtcatctg cacgacggtg gggccctatg
     1561 ctctgtatgg cagccggctc gttgctgcgt gcgtcagccg gggaacccac tactgcgatc
     1621 tgaccggcga ggtccactgg atgcgccgaa tgatcgacaa ccatcacctc agtgcccagc
     1681 aggccggggt tcggatcgtc cacacgtgcg gcttcgacag cataccgtcg gacattggcg
     1741 tgctctgcgt gcaacgggaa atgaaatccc ggcacggtga ggtggccacc gaggtcaagt
     1801 cccgcgtgcg cggaatgcgc ggcggcttca gcggcgggac cgtggccagc atgctcaaca
     1861 tgatggaaga aagcgagatg gacccgggcg ttcgggaggt aatgaacgat ccgtatggcc
     1921 tcaaccccgc gggcgagcgt cacggcctcg atgggcccga gcactcggtg cccgaatacg
     1981 acccggattt cggcgcatgg accacaccgt tcgtcatggc agccatcaac accaaggtcg
     2041 ttcgccgcag caacgcgttg tccggctacg cctacgggcg cgactttcgg tacgacgagg
     2101 cgatgctgac gggcttcggc ccctggggct tcccgctggc cgcggcgatg agcgtcggct
     2161 cgacggcttt caacgccgcc gccggggttt ctgcgctgcg tggattgctg gcgcgggttc
     2221 tgcccgagcc cggggagggg ccctcggccg ctgcccgcga gcggggattc ttcgacatcg
     2281 agatgctcgg caagagccgc cgcagcggtg cgcgggtcaa ggtcaggatc cgcggggatc
     2341 gtgatccggg ctacggctcg acctcgcgga tgctgggcga gagcgccgtc tgcctggcca
     2401 gagacgatct ggagacaccc acgggcgtgc ttaccccggc cgtggccatg ggcgaagcgc
     2461 tgatcgggcg tcttggcaac aacgccggcg tgacgttcga ggtgatgccg tgaggcgctc
     2521 acttcgtcgc tcatctaacg aaaggagcgc gtgggcgtga gcgagaccgg gccggctccc
     2581 caggaacgcg gcaaatccaa catcctcctt cggattgcga cctggctgat tactgccggc
     2641 tgcttctatc tcgtttacgg ccggacggcc gaagcggcgg cacgtgacgg cctgaccgtg
     2701 ttggaatacc tggcccgctt cttcggcgag gccaactggc ttgcctggct cgcggtcatg
     2761 gtcccctact ccgttttctt cttcgttgtc gacgctcacg tgacctggcg agtcatccgc
     2821 tggttcaatg cccccgaaat ccggctgggc aacgtgctgc cgatccgtgc cagcgcctac
     2881 atcctgtccc tggtcaacga gcaggtgggc aagggcgcga tgtcccttta cctctatcgt
     2941 cgttacaaag tccctgctct ggcggccatc tccagcatgc tgctgctcgg gctgatggaa
     3001 atctaccagt tgctggtgtt ctcctcggtc ggggtctttc tcggattcga ggtggtccag
     3061 gcggcctcca cggagctgcc gctcgacaag atcctgcccg gcgtgtttgc cgtggcggcc
     3121 atctatctgc cgctgcacat cgcctacttc cggggacatc tactgaaggg atcgacggtt
     3181 cgggagaaga gcctggtccg ggcattccgt gaagcccggc ccattcacta cctgctcatt
     3241 ttgctgttca aggcgccgct tctgatcggt gcgatcgtcg tctacaccat cgccttgaac
     3301 ctcttcaacg tcgacgtcag cttcgctcag atgctcgcct tccttcccgt gatctttctg
     3361 gccgcggcgc tgccgctgcc gttccatgcc ggcgcccttc ttttctggac cattcttttc
     3421 ccggactttc ccgaggtcgg tgctttcagc ctcgtgatgc acaccttctt cgtgctgttc
     3481 aacgcgttga tcggggtggt gttcctgccg atcgcgaatc gggagctatt cggatcggag
     3541 cagccaggct cgggcacggt cgcccatgga aacgatgctg gtgacgacga gcacccaggc
     3601 cagcccctgg atcagggcgg cgctgggcca cggtagggcc agcccctctt taacgatggg
     3661 tatgccggcg gcgaagaagt aggcgatgcc gttcattcgc ccgagatagc tcgtccgcag
     3721 gtgtttgccc gccagcgccc gggagtcgag ggcatactgt atgaaagccg cagcaaccag
     3781 gcaggggagc gcccaggtca ggtagccggc agtggccagg gcgcccagag cgaaggtgac
     3841 gaagagcgcg tccgtcgcgt ggtcgaacaa gcccccgacc gggctggcgt ggttcaagcg
     3901 tcgcgcgagc gggccgtcga gcaggtcggt gatcacggca agcgcgaaca gggctgcggc
     3961 cagcggccaa tcaccggaga caacggccat ggcgcacggc accgcaagcg ccaggcggac
     4021 ggtggtgagg agattcgccc aggtgaacca catggcgcga ttatgctacg ccagccaccc
     4081 atcggtgtcg ccttgctgag cgcgttcgcg cgctccagaa tgtggcccgt gatgcgcgtc
     4141 gaacaccaga gcctgccggt atcgcccgcc gatccatgga tcggcagggg ctgcccatga
     4201 tcgtcgtcac cggagcaaac ggcaatcttg gcaggcggct tctgcagagc ctctccggcc
     4261 gggccccgct gcgggccgtg gtgcgctcgg agcgggccgc cgagcagatc agggctctga
     4321 cgctggaacc cgaacctgag atatgcgtgg tcgattacca ggatgctgcc gccatgaccg
     4381 gggcgctcgc gggggcggaa tatgtcgtcc atctcgtagg tatcatcaag gagacggcgg
//
LOCUS       DBNHQR010000344         6207 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1552186, whole genome shotgun sequence.
ACCESSION   DBNHQR010000344 DBNHQR010000000
VERSION     DBNHQR010000344.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6207)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6207)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6207
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1552186"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1964)
                     /locus_tag="AC16VR_07585"
     CDS             complement(<1..1964)
                     /locus_tag="AC16VR_07585"
                     /inference="COORDINATES: protein motif:HMM:NF015155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HIR6011055.1"
                     /translation="MAGAWLATPLSRWVGGVSRRPWTVLVCIAVATAALGWVALDRFR
                     MNSNLSDLIRQEATWRVDFDRFKEQFPDLVETAVVVVSGTSYRDVEAMTGRLEAALLG
                     RDDLFRAVYAPENDPFFRRHALLYLDEDDLDDMADRLAEAQPMLTAVAEDPSLRGVLE
                     LVGDGIENEPAAGFDTVVRLLAESGEAVLAGGDPTVSWTDELFETDGTIHRLIFLKGQ
                     ADFQESLANARIMKELRALIAQLPAIDGVDVRITGEVALAHEEIEAAYAGVQIAGWVA
                     LALLMIILIVGVRSAKIIIATVLLLFVGVTWTSAFAMLTVGEYNTLSIVFLVMFFGLG
                     VDFALHFSLRYQEEINSGEGDALRSLVSATNSVGGAIAICTITTALGFLSFYPTPYKG
                     LADLGVISAGGMLVAGFLTFTLLPALYAVLGDIRPHIVDLPTGDRLVAYLSRHRGPVL
                     AALGLGAALALFLASRAHFDYSVLALKDPRAESVVTLRVLQADDAVTDYALTIMSARQ
                     APEAALEELPTVETVLSPADYVPDGQDDKLFVLEDLQELLWSALEPLRTADPPTGTEL
                     REAAEALTATVAAAQAAAPSSPDVDRLAAVLTGLLERPPQALLIWQRGVITNLLEELG
                     WLREALYVDALAFEDLPEKLRARLVSEEGQR"
     gene            complement(1966..2688)
                     /locus_tag="AC16VR_07590"
     CDS             complement(1966..2688)
                     /locus_tag="AC16VR_07590"
                     /inference="COORDINATES: protein motif:HMM:NF016236.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0120010 - intermembrane phospholipid
                     transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaA family lipoprotein"
                     /protein_id="HIR6011056.1"
                     /translation="MAKALMLAVAVLLGGCASLEGPEYGPYDPFDKFNRGSYKFSDAV
                     DKNVLIPVVKGYQAVTPQWLRNGIGNVFSNLRELDSALNGYLQLKPGSGTTDLARVAL
                     NTTVGIGGFFDVAATGGLIYQEEDLGQTLAVAGITRTRYVYVPLAGPTTIRDAPYSFI
                     RAWWPRLLLGSAYGWWMGIIDLLNTRAEVLAVTEARDASALDPYAFTREAYYQRRKFL
                     IYDGDPPMDDFFDDFEDEDEAF"
     gene            complement(2681..3298)
                     /locus_tag="AC16VR_07595"
     CDS             complement(2681..3298)
                     /locus_tag="AC16VR_07595"
                     /inference="COORDINATES: protein motif:HMM:NF017319.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6011057.1"
                     /translation="MRESMLGILAAAIWLLAAAAHGQAEADTADPGEDPSPRIESFHA
                     ALLEAMRSNDAYDVRFASLSPVVGEVFDVPTIARISLGRTWKSLDEPARAQFCELLEE
                     LITATYTDRFDSYSGQTFHLLSAEPARRGWVVKTELEKSDGERVSLDYYFRGSRVFNV
                     VADGVSDLSLRRADYNSIVKQEGYDRLLAHIRDSIAEYEAGGTDG"
     gene            complement(3295..4575)
                     /locus_tag="AC16VR_07600"
     CDS             complement(3295..4575)
                     /locus_tag="AC16VR_07600"
                     /inference="COORDINATES: protein motif:HMM:NF018513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR6011058.1"
                     /translation="MIWVGIVCVLIAAVVGAPLFAVLMAAAMLGFVASDIDLTIVAIE
                     VYRIVDTPLLMVLPLFTYSGYLLAEARTSERLVNLVQSLFGWLPSGLAMVGFVACAVF
                     TALTGASGVTIVALGALLLPALARSGYAEKFGLGLVTASGSLGLLLVPSVPLILYGIV
                     AQQLDVGEPFTIFDLFVAGVLPTLLMLTMLIGWTLWRHRGGVIPRTPFSRQRLWEAVK
                     AARWELPLPVIVLGGIYSGYFAISEAAAVTAVYVTLAEVILYREVTWRELPAVMRNSM
                     VMVGGILLVLGVALAFTNFLVDAEVPQKLFEFMQENIQSKIGFLLLLNLTLLVLGAFL
                     DIFSALVIVVPLILPVAVGYGIHPVHLGIIFLANMQIGYFTPPIGMNLFIASYRFNKP
                     LHELYMASLPFMLVLLVALLLITYVPWFSLVFVE"
     gene            complement(4572..5033)
                     /locus_tag="AC16VR_07605"
     CDS             complement(4572..5033)
                     /locus_tag="AC16VR_07605"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR6011059.1"
                     /translation="MIDVLSRWVRLVENGLLVGMLGVMIFLAAYQVIARNFFDSGLIW
                     GDSAVRIAVLWVTLLGAMVASRNDTHIRMDVISQFVDEAARKLLRRIGSVFTCVICGL
                     FAWYSYQFVVLDYQDEIIAFGRVPAWICEAIMPLGGAVMSFRYLLHVFRPA"
     gene            complement(5026..6039)
                     /locus_tag="AC16VR_07610"
     CDS             complement(5026..6039)
                     /locus_tag="AC16VR_07610"
                     /inference="COORDINATES: protein motif:HMM:NF015445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR6011060.1"
                     /translation="MNISRRWLRAAVCAVSLLAAAAPAGAVVFKIATDAPDGSLWVSS
                     LRSAAEQVVEHTQGRVTFKFYPGGVMGDDKAVMRKIRSGQLHGAVMTVGGLTRTYTDI
                     QLYNMPMAFRSLAEVDHVRANMDQTLLNGLEEHGFVSFGIAEVGFAYAMTQERIATLE
                     EARAQKVWVPDGDEGAARSLESFGITPIPLSIADVLGGLQTGLIDSVTVPPVAAIVLQ
                     WHTQLDHVLDLPLLYVYGTLAVSKRQFNKVSDEDQAYMRQVLGELVREVNAQNRQDHE
                     RAVEVLEEQGLAWHAADPVAVAQWQDLADAASLELVEAGIVSAPIYEDMVRLLVEYRD
                     SVD"
     gene            complement(6026..>6207)
                     /locus_tag="AC16VR_07615"
     CDS             complement(6026..>6207)
                     /locus_tag="AC16VR_07615"
                     /inference="COORDINATES: protein motif:HMM:NF028121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HIR6011061.1"
                     /translation="LTKVMFAEQYARLVFDRELHDRLLNEVIETDPVVPGLTLMNVVA
                     RQQAQELLESADEYF"
BASE COUNT         1244 a         2047 c         1945 g          971 t
ORIGIN      
        1 cgctgtccct cctcgctgac caggcgggcg cgcagcttct ccggcaggtc ctcgaaggcg
       61 agcgcatcca cgtacagggc ttcacgaagc caaccaagct cttccaaaag gttggtaatg
      121 acgccccgct gccagatcag cagcgcctgg ggcggccgtt cgaggagccc ggtcagcacg
      181 gcggccaacc ggtccacgtc cggcgaggac ggcgcggcgg cctgggctgc ggccaccgtc
      241 gcggtcaggg cctccgcggc ctcacgcagt tcagttcccg tgggcggatc ggcggttcta
      301 agcggctcga gcgcgctcca tagcaactcc tgcagatcct ccaggacgaa cagcttgtcg
      361 tcctgcccgt cgggaacgta atccgccggt gacagcacgg tctccaccgt cggcagttct
      421 tccagcgccg cctcgggcgc ctgccgcgcg gacatgatgg tgagcgcgta gtccgtcaca
      481 gcgtcgtccg cctgcaggac gcgcagcgtc acgacggact ccgccctggg gtccttcagc
      541 gcgagaacgc tgtagtcgaa gtgcgcgcgg gacgcgagaa agagagcgag ggctgcgccc
      601 aagccgagcg ccgcgagcac gggtcctcga tgtcgcgaga ggtaggcgac caggcggtca
      661 cccgtgggca ggtcgacgat atggggcctg atgtccccga gcacggcgta aagtgccggc
      721 agcagggtga aggtaagaaa cccggccacg agcatgccgc ccgcggagat cacgcccagg
      781 tcggccaggc ccttgtacgg cgtgggatag aagctcaaga accccagcgc ggtggtgatg
      841 gtgcagatgg cgatggctcc accgacgctg ttcgtggccg acaccagcga gcgcagggca
      901 tcgccctctc ccgaattgat ttcttcctgg taacgcagcg agaaatgcag cgcgaagtcg
      961 acgcccaggc cgaagaacat gacgaggaag acgatggaga gcgtgttgta ttcgcccacc
     1021 gtgagcattg cgaacgccga ggtccaggtc acgccgacga agagcaggag caccgtggca
     1081 atgatgatct tcgccgagcg cacgccgacg atcagaatga tcatcagcag ggccagcgcc
     1141 acccacccgg cgatctggac gccggcgtag gcggcctcga tctcctcgtg agcaagagcc
     1201 acctcgccgg tgatccgcac gtcgaccccg tcgatggccg gcagctgggc aatgagggct
     1261 ctgagctcct tcatgatacg ggcgttggcc agagactctt gaaaatccgc ctgacctttg
     1321 agaaagatca gccggtggat cgtcccgtcg gtctcgaaga gctcgtccgt ccaggagacc
     1381 gtcggatccc ctcccgcgag gaccgcctcg cccgattcgg cgagcagacg gaccaccgtg
     1441 tcgaacccgg ccgcgggctc gttctcgatc ccgtcgccga cgagctcgag cacgccccgc
     1501 aggctcggat cctcggccac ggcggtgagc atcggctgag cctccgccag ccggtcggcc
     1561 atgtcgtcga gatcgtcctc gtcgaggtac aacagcgcgt ggcggcggaa gaacggatcg
     1621 ttctccggcg cgtacacggc gcgaaaaaga tcgtcccggc cgagcagtgc agcctccagc
     1681 cgcccggtca tcgcctcgac gtcccgatac gaggttcccg aaaccaccac aacggcggtt
     1741 tcgacgaggt cggggaactg ctccttgaag cggtcgaaat ccacccgcca cgtggcttcc
     1801 tggcgaataa ggtcgctcag gttcgagttc atccggaatc gatcgagggc aacccagcca
     1861 agcgcggcgg tagccacagc gatgcagacc agcaccgtcc agggccggcg cgaaaccccg
     1921 cccacccagc gcgacagcgg cgttgccaac caggcgccgg ccattctaga aggcttcgtc
     1981 ttcgtcttcg aagtcgtcga agaagtcgtc catgggcgga tcgccgtcgt aaatcaggaa
     2041 cttgcggcgc tgatagtagg cttcacgggt aaaggcgtag ggatcgagcg ccgaggcgtc
     2101 tctcgcttcg gtcacggcca gcacctccgc acgggtattg agcaggtcga tgatgcccat
     2161 ccaccagccg taggcgctgc cgagcagcag gcgcggccac caggcccgga tgaaggaata
     2221 aggcgcgtcc cgaatcgtgg tcggcccggc gagaggcacg tagacgtaac gtgttcgggt
     2281 gatgccggca acggccagcg tctgccccag atcttcctcc tgatagatca ggccgcctgt
     2341 cgcggcgacg tcgaagaacc cgccgatgcc gacggtggta ttcagagcca ccctggccag
     2401 atcggtggtg cccgatccgg gcttgagctg aagatagccg ttcagcgcgc tgtccagctc
     2461 ccgcaagttg gagaacacgt tgccgatccc gtttctgagc cactgcgggg tgacggcctg
     2521 gtatcccttg accacgggaa tgaggacgtt cttgtcgacc gcatcggaga acttgtagct
     2581 gccgcggttg aacttatcaa aggggtcgta ggggccgtac tcgggcccct ccagcgaggc
     2641 gcagccaccc agcagcaccg caaccgcgag catcagcgct ttagccatcc gttcctcccg
     2701 cttcgtactc ggcaatgcta tcgcgaatgt gggccagcag ccgatcgtat ccctcctgct
     2761 tgacgatgct gttgtaatcg gccctgcgca gagagagatc gctcaccccg tccgccacga
     2821 cgttgaatac ccgacttccg cggaagtagt aatccaaaga cacccgctcg ccgtcagact
     2881 tctcgagttc ggtcttgacc acccagcccc ggcgcgcggg ctccgcgctc aagaggtgaa
     2941 aagtctggcc gctgtagctg tcgaaccggt cggtgtaagt cgcggtgatc agttcctcca
     3001 gcagctcgca gaactgcgca cgggcaggct cgtccaggga cttccaggtt cgtccgaggc
     3061 tgatgcgggc gatggtgggg acatcgaaca cctcccccac caccggcgac agcgacgcaa
     3121 agcgcacatc gtaggcgtcg ttggagcgca tcgcctcgag cagggccgca tggaagcttt
     3181 cgattctggg agacgggtcc tcacccggat ctgcggtgtc tgcttcggct tgcccgtgag
     3241 cggccgcggc gagcagccag atcgccgccg caaggatccc aagcattgac tctctcactc
     3301 gacgaacacc aggctgaacc agggcacata ggttatcagc aacagagcga ccagtaacac
     3361 gagcatgaac ggcaggctcg ccatgtagag ctcgtgcagc ggcttattga agcggtagct
     3421 ggcaatgaac agattcatgc caattggcgg cgtgaagtag ccgatctgca tgttcgccag
     3481 aaagatgatg cccaggtgaa cggggtgaat gccgtagccc acggccaccg ggagaatgag
     3541 cggcaccacg atgaccagcg ccgagaagat gtcgaggaag gcgccgagaa ccagcagcgt
     3601 caggttgagc agcagaagga agccgatctt gctttggatg ttctcctgca tgaactcgaa
     3661 cagcttctgc ggcacctccg catcgaccag aaagttggtg aacgccagcg ccacgcccag
     3721 aaccagcagg atcccgccca ccatcaccat ggaattgcgc atgacggcgg gcagctcgcg
     3781 ccaggtcacc tcgcggtaga ggatcacctc ggcgagcgta acgtaaacgg cggttaccgc
     3841 cgcggcttcg gagatcgcga agtacccgga gtagatgccg cccagcacga tcaccggcag
     3901 cggcaattcc caacgcgccg ctttgactgc ctcccacagg cgctgcctgg agaagggcgt
     3961 tcttggaatg acccctcccc gatggcgcca cagtgtccag ccgatcagca tcgtcagcat
     4021 cagcagcgtg ggcagcacgc cggcaacgaa caggtcgaag atcgtgaacg gctctcccac
     4081 gtccagctgt tgagccacga tgccgtagag gatgagcggc acggagggaa cgagcagcag
     4141 ccccaggctg ccggaagcgg tcaccaggcc cagcccgaac ttctccgcat accccgaccg
     4201 cgccagggca ggcaggagca gggcgcccag cgccacgatg gtcacgccgg acgcgccggt
     4261 cagcgccgtg aacaccgcgc aggcgacgaa ccccaccatg gccagaccgc tcggcaacca
     4321 accaaagagg ctctgcacga ggttcacgag ccgctcggag gtgcgcgcct ccgccagcag
     4381 gtagccgctg tatgtgaaca ggggcagcac catcagcaga ggcgtgtcga cgatccggta
     4441 gacctcgatg gcaacgatcg tcaggtcgat gtccgaagcg acgaacccaa gcatcgcggc
     4501 ggccatcagc acggcgaaga ggggcgcccc gaccacggcg gcgatcaaca cgcagacgat
     4561 cccgacccag atcatgcggg cctgaacacg tgcaggagat aacggaagct catcacggcg
     4621 ccgcccaacg gcatgatggc ttcacagatc catgccggca cccggccgaa cgcgatgatc
     4681 tcgtcctgat agtccaagac gacgaactgg tagctgtacc aggcgaacag gccgcagatg
     4741 acgcaggtga acacgctgcc gatccgccgc agcagctttc gcgccgcctc gtctacgaac
     4801 tgcgagatga cgtccatgcg aatgtgggtg tcgttgcgcg aagcgaccat cgcgccgagc
     4861 agcgtcaccc agagcacggc gatgcgcacg gcggaatcgc cccagatgag accggaatcg
     4921 aagaagtttc tggcgatgac ctgatacgcc gccagaaaga tcatcacgcc gagcatcccg
     4981 accagcagcc cgttctcgac gagccgcacc cagcgtgaga ggacgtcaat caacgctgtc
     5041 tcggtattcc acgagaaggc gaaccatgtc ctcgtagatc ggcgcggata cgatgccggc
     5101 ttcgaccagc tccagagaag ccgcatcggc gagatcctgc cactgggcca ccgcgaccgg
     5161 atcggcagca tgccacgcaa ggccctgctc ctcgagcacc tccacggcac gttcgtggtc
     5221 ttgccggttc tgggcgttga cctcgcgaac cagctcgccc aggacctgcc gcatgtaggc
     5281 ctgatcctcg tccgagacct tgttgaactg acgcttgctg actgccagcg tgccgtagac
     5341 gtacagcagg ggaagatcca gcacatgatc gagctgggtg tgccactgca gcacgatggc
     5401 cgccaccggg ggcaccgtca cgctatcgat caggccggtc tgaagccctc ccagaacgtc
     5461 ggcgatgctt aacgggatgg gcgtgatgcc gaaagattcc agcgacctcg cggccccttc
     5521 gtcgccgtcc ggcacccaca ccttctgcgc ccgcgcttct tccagggtgg cgatgcgttc
     5581 ctgggtcatg gcgtaggcaa agcccacttc ggcgatcccg aacgaaacga agccatgctc
     5641 ctcgaggccg ttaagtagcg tctggtccat gtttgcgcga acgtgatcga cctcggcgag
     5701 ggacctgaac gccatgggca tgttgtacag ctgaatgtcc gtgtaggtgc gggtcaggcc
     5761 acccacggtc attacggccc catgcagctg cccggagcgg atcttgcgca tcaccgcctt
     5821 gtcgtctccc atgacgccgc ccgggtagaa cttgaaggtg acacggccct gcgtgtgctc
     5881 gacaacctgc tccgcagcag acctcagcga gctcacccac agggaaccgt ccggcgcatc
     5941 ggtggcgatc ttgaacacca ccgctccggc aggcgctgct gccgcgagca gggaaaccgc
     6001 gcatacggcc gcgcggagcc atcgtctaga aatattcatc cgccgattcc agaagctcct
     6061 gtgcctgttg ccgtgcaacc acgttcatga gcgttagacc cggcacgacc ggatccgttt
     6121 cgatcacctc gttcagcaga cggtcatgca gctcgcggtc aaataccagc cgcgcgtact
     6181 gttccgcgaa catcaccttg gtcagaa
//
LOCUS       DBNHQR010000345         4214 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_222203, whole genome shotgun sequence.
ACCESSION   DBNHQR010000345 DBNHQR010000000
VERSION     DBNHQR010000345.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4214)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_222203"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..755
                     /gene="ftsE"
                     /locus_tag="AC16VR_07620"
     CDS             63..755
                     /gene="ftsE"
                     /locus_tag="AC16VR_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017520095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HIR6011062.1"
                     /translation="MGQIEFHHVTKRFDDGFEALKDVSVDFPEGSMTFLTGHSGAGKS
                     TFLKLLLCADRPSAGQIIVNGVNVRHISARRLAHYRQHIGVVFQDHHLLHHRDVYENV
                     ALPLRVAGMRERAVARRVRASLTRVGLLVKERMFPRYLSTGEQQRVGIARAVATRPRI
                     LLADEPTGNLDPELSRDIMHVFEQVNQVGTTVIIASHDRDLIESMGLRIIELAHGNIV
                     RDTDREADIGFE"
     gene            841..1758
                     /locus_tag="AC16VR_07625"
     CDS             841..1758
                     /locus_tag="AC16VR_07625"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HIR6011063.1"
                     /translation="MGLVAWWQDHLRTGRETLHFISARLGTSLLVWLLVGIALALPAG
                     LILLQLNLAGMTADWDGRPGLSVYFELNTDAGKVAAIAQRLSERAEIEGVRVTSPEEA
                     LAEFQSHADVGDALDLLTENPLPASLRATLVPGSSLLDLELVAGLVEGAPGVSEVVVE
                     KTWLERVIDISRVVSRLGIILGALFGVGAVLVTATSVRLAIEARLEELRVLKLVGATD
                     RQIRRPFMYFGALYGLGGGLVAAMLISLCLVAIETPVSNLLGSYGRDLETPGFDPTFL
                     SGLLLVGAFLGVSGALLAARQRLKDLEIL"
     gene            1849..2712
                     /gene="rpoH"
                     /locus_tag="AC16VR_07630"
     CDS             1849..2712
                     /gene="rpoH"
                     /locus_tag="AC16VR_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008175688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HIR6011064.1"
                     /translation="MSTDVLSMKSLAPGRDLDAYVQTVSAFPVLSAEQEKDLAERFYY
                     DNDLEAARELVLCHLRFVVHLARSYRGYGLPQADLIQEGNVGLMKAVKRFNPEVGVRL
                     VSFAVHWIKAEMHEFILRNWRIVKVATTKAQRKLFFNLRSSKKRLAWLTQEETRAMAR
                     ELGVSEEEVARMEGRMAASDLAFDGYASDDDSSPLAAPVHYLEHEGSNPEDLVAEEDW
                     HDEASARLGAALAELDERSRAIVEARWLAEKKATLHELGDTYGVSAERIRQIEQNAIK
                     KLKAACMSDAA"
     gene            2735..3109
                     /locus_tag="AC16VR_07635"
     CDS             2735..3109
                     /locus_tag="AC16VR_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008568527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HIR6011065.1"
                     /translation="MPANVPLAAGALFGLTGVVMGALGAHGLAGQLSESGVDIWDTAV
                     LYHLTHALALVAVGVRLGQKVPPPWPVVLAGWGFGVGILLFSGSLYVLALGGPPVFGP
                     LTPLGGLALCAGWVALLAAARR"
     gene            3129..3809
                     /gene="trmB"
                     /locus_tag="AC16VR_07640"
     CDS             3129..3809
                     /gene="trmB"
                     /locus_tag="AC16VR_07640"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF014447.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HIR6011066.1"
                     /translation="MEERTYLRRRGRMTRGQARALDRAGDTMIVDSTSPDWPAVFGRQ
                     APLGVEVGFGMGHALLDWAAECPDWNLVGIEVYQPGIGALLLGLDSQGSTNVRVLEAD
                     ARQALAAGFEPDSIDEVRILFPDPWPKKRHHKRRLVQPDFVRLLASRMRPGARLGLAT
                     DWQDYAQHMLTVLDAEPLLQNRSGQGSYAERPSGRPTTRFEERGRRLGHQVWDLGYER
                     KRETTESR"
     gene            complement(3773..>4214)
                     /locus_tag="AC16VR_07645"
     CDS             complement(3773..>4214)
                     /locus_tag="AC16VR_07645"
                     /inference="COORDINATES: protein motif:HMM:NF018652.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HIR6011067.1"
                     /translation="EQAGFERYEVSAYARPGRRSRHNVNYWSFGDYVGIGAGAHGKLT
                     SGGRVVRTVKPSQPRLYLQDPESQQRQIVEAEALPFEFMLNALRLRDGVPLDQFTQRT
                     GLALEALDPVWGELVDEGLVHRHRLATTPEGYRYLDSVVSRFLS"
BASE COUNT          695 a         1283 c         1445 g          791 t
ORIGIN      
        1 cccttcgatg ccaaagattt cgtcgacgcc cttctcgcgc ccgagtaagc aaagcgggca
       61 ccatgggaca gatcgagttt catcacgtca ccaaacgatt cgacgacggc ttcgaagcgc
      121 tcaaggacgt cagcgtcgac tttcccgaag gctccatgac ctttctgacc gggcactccg
      181 gggccggcaa gagcacgttt ctgaagcttt tgctgtgtgc cgatcgcccg tcggctgggc
      241 agatcatagt caacggagtg aacgtccggc acatcagcgc caggcgtctc gctcactacc
      301 gacagcacat cggcgtggtt ttccaggatc atcacctgct gcatcatcgt gacgtgtacg
      361 agaacgtagc gctgccgttg agggtcgccg gaatgcgcga gagggcagtg gctcggcggg
      421 tcagggcgtc gctgacccgg gtcgggctgc tggtgaagga gcggatgttt ccgcgttacc
      481 tttccaccgg tgagcagcag cgggttggca ttgcccgggc ggtggccaca cggccgagga
      541 tccttctggc ggacgagccc acgggcaatc tggatcccga gctctcccgg gacatcatgc
      601 acgtcttcga gcaggtcaat caggtcggca ccacggtgat catcgcgagc cacgatcgag
      661 acctgatcga gtccatgggg ctgcgcatca ttgagcttgc ccacggcaac atcgtcagag
      721 atacggacag agaagcggac attggcttcg aataatgagg cgcaaggggg tcggcccgcg
      781 ccggccacgg gcggcgggcg aggccgcacg aagaagggat atccctggcc ccaccgccgc
      841 gtggggctgg tggcctggtg gcaggaccat ctgcgcacgg gacgggagac gctgcatttc
      901 atcagcgccc gtctgggaac gagcctgctg gtctggttgc tggtggggat cgcgctggct
      961 ctgcccgcgg ggctgattct gcttcagctc aacctcgccg gcatgacggc cgattgggat
     1021 gggcgaccgg ggctgtctgt gtatttcgag ctgaacaccg acgcaggaaa ggttgcggcg
     1081 attgcccaac ggctgtccga acgtgccgag atcgagggtg tcagggtgac ctcgcccgag
     1141 gaagcgctgg ctgaatttca aagtcacgcg gacgttggag acgctctcga cctgctgacg
     1201 gagaacccgt tgccggcctc gctgcgcgcc accctggtgc cggggtcttc gctgctcgat
     1261 ctggagctgg tcgcggggct tgtcgaggga gcgccgggag tatcggaggt ggtggtcgag
     1321 aagacctggc tggagcgggt catcgacatt tcccgggtgg tcagccgcct ggggatcatt
     1381 cttggggcgc ttttcggtgt cggcgcggtg ctggtcaccg caacttccgt gcggctggcc
     1441 atcgaggcgc ggctggagga gctcagagtg ctgaaactgg ttggggccac cgaccgacag
     1501 atccgtcggc cctttatgta cttcggcgcg ctttacgggc ttggtggcgg tctcgtggcc
     1561 gccatgctga tcagcctttg cctggttgcc atagagacac cggtctcgaa tctcctcgga
     1621 agctacggcc gtgaccttga aacgccgggg ttcgacccga ccttcctttc cgggctgctg
     1681 ctggtcggcg ctttcctggg agtgtccggg gcgcttctgg ctgcgcggca gcgtctgaag
     1741 gatctcgaaa tcctttagtt ttcggcgggt tagggaacca ttcgcggggt tagcagtctc
     1801 accaccagag tgctaaactg ctgaaattag tgttaccgga ggtatcacat gagcaccgac
     1861 gtgctttcta tgaaaagtct cgccccggga cgcgatctgg atgcttacgt tcagacggtc
     1921 agcgcgtttc cggttctttc tgcagaacag gagaaggatc tggcggagcg gttttactat
     1981 gacaacgacc tggaagccgc ccgggaactg gtgctctgcc atctgcgctt cgtcgtccac
     2041 ctggcccgtt cgtacagagg ctacgggctg cctcaggcgg acctgatcca ggagggcaac
     2101 gttggcctga tgaaggccgt caagcggttc aatcccgagg tgggcgttcg cctggtctct
     2161 tttgccgtgc attggatcaa ggcggaaatg cacgagttca tcctgcgtaa ctggcggatc
     2221 gtcaaggttg ctaccaccaa ggcccagcga aagctcttct tcaacctgcg cagctccaag
     2281 aagcggctcg cctggctcac ccaggaagag acccgtgcca tggcccggga gctgggcgtt
     2341 tccgaggaag aggtcgcgag gatggagggc cggatggcag cgtccgacct ggccttcgac
     2401 ggctatgctt cggacgacga ctcctcgccc ctggccgccc ccgtccacta tctggaacac
     2461 gaaggttcga atcccgagga cctggtggca gaggaagact ggcacgacga agccagtgcg
     2521 cgtctgggcg ccgcgctggc cgagctggat gagcgaagcc gggcaatcgt ggaagcccga
     2581 tggctcgccg agaagaaggc gacgctccac gagttgggcg acacatacgg cgtgtcggcg
     2641 gagcggatcc gccagatcga gcagaatgcg atcaagaagc tgaaagccgc gtgtatgagc
     2701 gacgccgcct gatccgctgg gcatcggggg tacgatgccg gccaacgttc ctttagccgc
     2761 gggagccctc ttcggcctga ccggcgtagt gatgggcgcc cttggcgccc acgggcttgc
     2821 cgggcaactg tctgaaagcg gcgtcgatat ctgggacacc gccgtgctct accatctcac
     2881 gcacgcccta gccctcgtcg ccgtgggcgt gcgcctgggc cagaaggtgc cgccgccgtg
     2941 gccggtcgtt ctggccggct gggggtttgg tgtcggcatc ctgctattct ccggcagcct
     3001 ttacgtgctc gctctgggcg ggccacccgt cttcggccct ctgacgcccc tgggtggcct
     3061 cgcgctctgt gccggctggg tcgccctgct cgccgccgcc cgccgctgag cgccgctcgg
     3121 gacacgcatt ggaagaaagg acttacctgc gccgccgcgg gcggatgact cggggtcagg
     3181 ctcgggccct cgaccgagcg ggcgacacga tgatcgtcga ctcgacatcg cccgactggc
     3241 ctgcggtgtt cggccggcaa gccccgctgg gggtggaggt cggcttcggg atgggacatg
     3301 ccctgctgga ctgggcggcc gagtgcccgg attggaacct ggtggggatc gaggtctatc
     3361 agccggggat aggcgctctg ctgctgggcc tggactccca gggctcgacc aacgttcgcg
     3421 tgctggaagc ggacgcgagg caggcgctgg ctgcgggctt tgagcccgac tcaatcgacg
     3481 aggtgcggat actctttccg gatccctggc ccaagaaacg tcaccacaag cgccggttgg
     3541 tgcagcccga tttcgtccgt ctccttgcgt cgagaatgcg gcctggggct cggctcggct
     3601 tggctaccga ctggcaggac tatgcccagc acatgctgac cgttctcgat gcggagccgt
     3661 tgctgcagaa ccgatcgggg cagggttcct atgccgagcg cccttcgggt cgcccgacca
     3721 cgcgcttcga ggagcgtggc cggcggctcg ggcatcaggt gtgggatctt ggctacgaaa
     3781 gaaagcgcga gaccaccgag tccaggtagc gatagccctc gggggtggtt gcgaggcgat
     3841 ggcggtgaac gaggccttca tccactagct cgccccagac cggatcgaga gcctccaggg
     3901 caagccccgt gcgctgggtg aactggtcca gggggacacc atcgcggagg cgaagcgcgt
     3961 tgagcatgaa ctcgaagggc agagcttcgg cttcaacgat ctgccgctgc tggctttcgg
     4021 ggtcctgcag gtaaaggcgc ggctggctgg gctttaccgt gcggaccacc cgaccaccgc
     4081 ttgtcagctt gccgtgggcg cccgctccga tcccgacgta gtcgccgaac gaccagtagt
     4141 tcacgttgtg acgcgatcgc cgacctggcc tggcgtacgc tgagacctcg tagcgctcga
     4201 agcctgcctg ctcg
//
LOCUS       DBNHQR010000346         3556 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1046364, whole genome shotgun sequence.
ACCESSION   DBNHQR010000346 DBNHQR010000000
VERSION     DBNHQR010000346.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3556)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1046364"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..1021
                     /locus_tag="AC16VR_07650"
     CDS             257..1021
                     /locus_tag="AC16VR_07650"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024809.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6011068.1"
                     /translation="MSNPTQPLKLYGVPFSQPVRAVMWLMLHKRMPFEMVLTNPGTSG
                     ENGSRHPDYLAKNPGGTIPTIEEPDTGFTLGEAHAILCYLCNKYGWSDMYPAEPQERA
                     RVDWYLHYHHRNVREASTGLVAPKIRKDLNIPETVQAAAQATLTRALQAIETGWLGKS
                     AFLAGDAVTIADFAAYVEIGQLQPGFTNVYDFDPFPNVRRWMDSLKTFDSHDDVHVVL
                     AELGDISAEPPSMEAIKNANISALRTLKARLAEMSG"
     gene            1056..1484
                     /locus_tag="AC16VR_07655"
     CDS             1056..1484
                     /locus_tag="AC16VR_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007947151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR6011069.1"
                     /translation="MSETHKGACFCGAVQIEVTGEPNMMGYCHCTDCAAWAGAPVNAF
                     SLWPPENVQVTAGEEKLGTYAKTEGSQRKFCTQCGGHVFTDHPGMGLIDVYLNVVPGK
                     AHTGAMHVFYGEKTMSVPDGLPKYRDLPKEFGGSGETIAE"
     gene            complement(1552..2316)
                     /locus_tag="AC16VR_07660"
     CDS             complement(1552..2316)
                     /locus_tag="AC16VR_07660"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6011070.1"
                     /translation="MGKNAESEPPLCTSWQRGRTESELVLMSDDLLDNPAFAALQHAH
                     AGLGRASELAAVYDPLVSPLAGLSEATPEALLDLAGLVPSGAGVGVLSTQPRLPESAG
                     WRLLRVMPLHQMVCETPPGQPEPFDELTPSDAPEMVALAELTEPGPFLERTIEMGRYV
                     GVRSGGRLVAMAGERMKLPGCTEVSGVCTHPDARGRGLALRMVLNVLAECFARGDRAF
                     LHVVAGSPSETTAIGIYERLGFEHRRPIYAHVLLRE"
     gene            complement(2384..3214)
                     /locus_tag="AC16VR_07665"
     CDS             complement(2384..3214)
                     /locus_tag="AC16VR_07665"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6011071.1"
                     /translation="MAFEAIRYEKTDGVATITKNNPPQYGMTLAVLTEMRAALIDANN
                     DADVCVVVITAGGEGFHMGAVVFGEARSDWKFSPLEFREVIQVGHDLFRLIETLEKPV
                     VGVAKAGAVGGGFENLHACDFVIASEAARFSQPEATLGMICGWGGTQRVPRMVGWRKA
                     KEILMEGVELTGREAAEIGLITRAVPLDEVDEAVSALCERLKICGPVALGYTKLAMNK
                     VWETDYRSGLDYEVEASGMVNSAGEFTAEVFEDFLAGRQPKFAKRQRLTAGREWRGTD
                     "
     gene            complement(3326..>3556)
                     /locus_tag="AC16VR_07670"
     CDS             complement(3326..>3556)
                     /locus_tag="AC16VR_07670"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6011072.1"
                     /translation="GFAIAAVGQDAEPAFAPGRHTGIGWGVESLDDAYETLRSTGVEF
                     TAPPERQPWGGYMALFKDPDGNVFYLDELREH"
BASE COUNT          698 a         1198 c         1068 g          592 t
ORIGIN      
        1 ccggccacga cgacggcaac gagcacgcct gctgcaatca tcaggacctt caacatgatg
       61 ggagcttcgt tcgggcgacg agcatggcgg tcatggtctt tcctttctcc tcgaacctgt
      121 attcaaccat gctcgcgaga cggtgaaatg cacgtgaatc ccccgcgccg gaatgcgacg
      181 ggactgcggt gcagcctcgg gagcttatca ccggacagaa ggcatgatag cttggtcgtc
      241 cgatcaggga ggaatgatga gcaaccccac ccaaccactg aaactctacg gcgtgccgtt
      301 ctctcaaccc gtccgcgccg tcatgtggct gatgcttcac aaacgtatgc cgttcgaaat
      361 ggtactcacc aaccccggca catcgggcga gaacggcagt cgtcacccgg actatctggc
      421 caagaatccg ggcggcacca tcccgaccat cgaggagccc gataccggct tcaccctggg
      481 cgaagcgcac gccatcctct gctatctgtg caacaaatac ggctggtcgg atatgtatcc
      541 agccgagcct caggagcgcg cccgcgtgga ctggtacctg cactatcacc atcgcaacgt
      601 cagagaggca tcgaccgggt tggtggcgcc gaagatccgg aaagatctga acattccgga
      661 gaccgtccag gcggccgccc aggccaccct gacccgggcg ctgcaggcca tcgagaccgg
      721 ctggctcgga aagtccgcat ttctcgccgg cgatgcggtc accatcgccg acttcgccgc
      781 ctacgtggag atcggccaac tgcagcccgg cttcaccaac gtctatgatt tcgacccgtt
      841 tccaaacgtt cgccgctgga tggactcgct caaaaccttc gacagtcacg acgacgtcca
      901 tgtcgtgctc gccgagctgg gcgacatcag cgccgagccg ccttccatgg aggcaatcaa
      961 gaacgccaac atctccgctc tgagaacgct caaggcgcgc ctggcggaga tgagcggatg
     1021 acccggcaat cctgaacacg agcaaaggaa taatcatgag cgaaactcac aagggcgcct
     1081 gcttctgcgg agcggtccag atcgaggtca ccggagagcc caacatgatg ggttactgcc
     1141 actgcacgga ttgcgcggcc tgggccgggg ccccggtgaa cgcgttcagc ctgtggccgc
     1201 cggaaaacgt ccaggtaacg gcgggcgagg agaagctggg cacctatgcc aaaaccgaag
     1261 gcagccagcg caagttctgc acgcaatgcg gcggccacgt gttcaccgac catcccggga
     1321 tgggactaat cgacgtttat ctcaacgtcg tgcccggtaa ggcccacacg ggcgccatgc
     1381 acgtcttcta cggcgagaag acgatgtcgg taccggacgg tttgccgaaa taccgggatc
     1441 ttccaaaaga gttcggcggt tccggcgaaa ccatcgccga gtaggaccgc cgcgagcggt
     1501 gagcggagga cccggggtcc ggtctgcacg ggccgccgat tgagacacgg tttactcgcg
     1561 cagcagaacg tgggcgtaga tcggcctgcg gtgttcgaag ccgagcctct cgtagattcc
     1621 gatggcggtg gtctcgctcg ggctgccggc caccacgtgc agaaacgccc gatcgccacg
     1681 ggcaaagcat tcggccagca cgttcagaac cattcgcaag gccagccccc gaccgcgcgc
     1741 gtcgggatgg gtacacacgc cgctgacctc cgtgcagccg ggcagtttca tgcgctcgcc
     1801 agccatggcg accagccggc cgccgctgcg cacgcccaca tagcgcccca tctcgatggt
     1861 ccgctccaga aacggcccgg gctcggtcag ctcggcaagg gcaaccatct ccggagcgtc
     1921 ggacggcgtc agctcgtcga agggctccgg ctgccccggc ggcgtctcgc agaccatctg
     1981 gtgcagcggc atgacccgca gcagccgcca gcccgcggat tcggggagcc ggggctgggt
     2041 cgaaagaacg ccgacgccgg cgcccgatgg caccagaccg gcaaggtcga gcagggcttc
     2101 aggcgtcgct tcggaaaggc cggcgagcgg cgataccagc gggtcgtaga cggcagccag
     2161 ctcgcttgcc cgaccaagac cagcgtgagc gtgttgcagc gccgcgaaag ccggattgtc
     2221 gagcagatcg tctgacatca gcaccagctc cgattcggtg cggcccctct gccacgacgt
     2281 gcaaagcggc ggctcacttt ctgcattctt gcccatcgag ccgaagatag ttcgagccag
     2341 ggggcgtgtc taacggccct cgcccggcaa taggtgtgcg ctgctagtca gtcccccgcc
     2401 attcccgtcc agcggtaagc cgctggcgtt ttgcgaattt cggctgccgc ccggcgagaa
     2461 aatcttcgaa cacctcggca gtgaactccc cggcgctgtt gaccataccg gacgcctcta
     2521 cctcgtagtc caggcccgag cggtagtccg tttcccagac cttgttcatc gcaagcttgg
     2581 tgtagccgag cgccaccgga ccgcagatct tcaacctttc acagagggcg gagacggcct
     2641 catccacctc gtcgagcgga accgcacggg tgatcaagcc gatctccgcg gcttccctgc
     2701 cggtcagctc cacgccttcc atgaggatct ccttcgcctt gcgccagccc accattctcg
     2761 ggaccctctg ggtaccgccc cagccgcaga tcattccgag cgtcgcctcc ggctggctga
     2821 agcgcgcggc ctcggaggcg atgacgaaat cgcaagcgtg gagattttcg aagcctccgc
     2881 ctacagcccc cgccttggcc acgcccacga cgggtttctc cagcgtctcg atcaggcgga
     2941 aaagatcatg tcccacctgg atgacctcgc gaaactccag gggggagaac ttccagtcgc
     3001 tgcgcgcctc gccgaaaacc acggccccca tgtgaaagcc ctcgccccct gcagtgatga
     3061 ccacgacaca aacgtcagcg tcgttgttgg catcgatgag cgcagcccgc atctccgtca
     3121 gcacggcaag cgtcatgccg tactgcggcg ggttgttctt ggtgatggtg gcaacgccgt
     3181 ccgtcttttc gtagcgaatg gcctcgaacg ccatatggtt ttcctcccag gggtttgcgc
     3241 ctataaacgc ccagaccagc acgaagagcg tgcagccgag ctggtcgacc cccagcgcgc
     3301 gatgcaacgc aaggagagcc ggccctcaat gctcccgaag ctcgtccaga tagaagacat
     3361 tgccgtcggg atctttgaac aacgccatgt aaccgcccca cggttgtcgc tcggggggag
     3421 ccgtgaactc gacccctgtg ctgcgaagcg tttcgtaggc atcgtccaga ctttcaactc
     3481 cccagccgat cccggtatgt cgccccggag caaaggccgg ttcagcgtcc tggccaaccg
     3541 cggcaatggc gaaccc
//
LOCUS       DBNHQR010000347         3860 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1171887, whole genome shotgun sequence.
ACCESSION   DBNHQR010000347 DBNHQR010000000
VERSION     DBNHQR010000347.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3860)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1171887"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1749
                     /gene="sdhA"
                     /locus_tag="AC16VR_07675"
     CDS             <1..1749
                     /gene="sdhA"
                     /locus_tag="AC16VR_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HIR6011073.1"
                     /translation="FDGVIVGGGGAGMRASLQLARSGLKTAVISKVFPTRSHTVSAQG
                     GITCAIASDDPNDDWRWHMYDTVKGSDYIGDQDAIEYMCSQGPQAVFELEHMGLPFSR
                     TEQGRIYQRPFGGQSKDFGKGGQAARTCAAADRTGHTLLHTLYQANLRANTTFLNEWF
                     AVDLVKNQDGAVVGIIAIEMETGETVFVSSKATVLATGGAGRIYASTTNAHMCTGDGA
                     GMALRAGFPMQDMEMWQFHPTGIAGAGTLVTEGCRGEGGYLINKDGERFMERYAPTAK
                     DLAGRDVVARSMILEILDGRGCGPDRDHVMLKLDHLGEEVLNSRLPGILELSRTFAHV
                     DPVKEPIPVVPTCHYMMGGIPTGVLGQALTQDAQGDDREIPGLYAAGEAACVSVHGAN
                     RLGGNSLLDLIVFGRAAGLHIEEVMRQGVSYRDPSDSDLEQAQARLNRWDESGPGETV
                     ADLKKELQTTMQNSFGVFRTGTHMEEGIKKIAELRDRISRAHLGDKSKVFNTARLEAL
                     ELDNLLEVAEATAIAANARTESRGAHAREDFQVRDDENWLCHSLFFSDERRVTKRAVN
                     FSPETVDTFQPMERKY"
     gene            1758..2465
                     /locus_tag="AC16VR_07680"
     CDS             1758..2465
                     /locus_tag="AC16VR_07680"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010166250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (quinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HIR6011074.1"
                     /translation="MMQVSVYRYNPETDEVPAMREMSVELPGDRDLMVLDVLEMLKAE
                     DPTLSYRRSCREGVCGSDGINMNGRNGLACITPVSSVAKRGKLVLRPLPGLPVIRDLV
                     VDMTQFYRQYEKIKPYLINDKPPPAQERLQSPEERAELDGLYECILCACCSTACPSFW
                     WNPDKFIGPAGLLQAYRFLADSRDTATAERLSELDDPFSVFRCRGIMNCVSVCPKGLN
                     PTRAIGKIRTMLLQQGT"
     gene            2554..>3860
                     /locus_tag="AC16VR_07685"
     CDS             2554..>3860
                     /locus_tag="AC16VR_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018925108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR6011075.1"
                     /translation="MADSFRERMERSSHLAGANAAYIEAMYERYLDEPNEVPPEWREY
                     FDTLPRVEGAVGADVPHSTIVDQFERLGRNRLKARPERESHFAQNVHEQKQMRVQDLI
                     SAYRHRGHKKANIDPLGMMERPAMPILDLAYHHLSLADLDETFNVGSFHYGSPEAKLR
                     DLVDALERTYCASIGFEYMHIVDQSEQLWVQQRLESVRARPVLTEDHKRVILERLTAA
                     EGLEKYLHGRYPGTKRFGLEGGESLIPMLHELLQRLGSHGVLEAVIAMAHRGRLNVLV
                     NILGKDPRLLFDEFDGKLPVNEDDMGDVKYHQGFSSNVMTPGGEMHLALAFNPSHLEI
                     VGPVVEGSVRARQDRRRDHVGTRVVPILVHGDASFAGQGVVMETLQMSQTRGFKTGGT
                     IHIIINNQIGFTTHRQDDARSTEYCSEVAKLVQAPIFHVNGDDP"
BASE COUNT          745 a         1211 c         1231 g          673 t
ORIGIN      
        1 tttgacggcg tcatcgtcgg tggcggcggc gcgggtatgc gggcgtctct tcagctggcg
       61 cggtcgggcc tgaaaacggc cgtgatctcc aaggtatttc cgacgcgctc ccacacggtt
      121 tccgcccagg gtgggatcac gtgcgccatc gccagtgacg atccgaacga tgactggcgc
      181 tggcacatgt acgacacggt gaaggggtct gactacatcg gtgatcagga tgccatcgag
      241 tacatgtgca gccagggacc ccaggcggtt ttcgagctcg agcacatggg gctgccgttt
      301 tcccgaaccg agcagggccg catctatcag cggccctttg gcgggcagtc gaaggatttc
      361 ggcaagggcg gccaggcggc ccgaacctgc gcggccgctg acaggacggg tcataccctt
      421 ctgcatacgc tctaccaggc caacctgcgg gcaaacacca catttctgaa cgaatggttc
      481 gccgtggatc tggtcaagaa ccaggacggc gccgtcgtcg gcatcatcgc catcgagatg
      541 gagaccggag aaacggtctt cgtcagctcc aaggccaccg tgctggccac gggaggcgcg
      601 ggccggatct acgcgagcac caccaacgct cacatgtgca ccggcgatgg agccggaatg
      661 gcgctcaggg ccggtttccc catgcaggac atggagatgt ggcagttcca tcccaccgga
      721 atcgccgggg ccggtaccct ggtgaccgag ggttgccgcg gcgaaggcgg ctacctcatc
      781 aacaaggacg gcgaacgctt catggagcgc tacgcgccta ctgcgaagga tctcgcgggc
      841 cgcgacgtgg tggcccggtc aatgatcctg gagattctcg acggtcgcgg ttgtggcccg
      901 gacagggacc acgtgatgct caagctcgat catctgggag aagaggtgct caacagccgc
      961 ctgccgggaa tcctcgagct gtcccgcaca tttgctcatg tggatcccgt caaggagccg
     1021 atccccgtag ttcccacctg ccattacatg atgggcggta ttcccaccgg cgtgctcggc
     1081 caggcgctta cccaggacgc ccaaggcgac gaccgcgaaa tccccggtct ttatgctgct
     1141 ggagaggccg catgcgtctc cgtgcatggc gccaaccgac tcggcggcaa ctcgctgctc
     1201 gacctcatcg tgttcggtcg ggccgcgggg ctgcacatag aggaagtgat gcgtcagggc
     1261 gtttcctatc gggatcccag cgacagtgat ctcgagcagg ctcaggcccg tctgaaccgc
     1321 tgggacgaaa gtggtccggg cgaaacggtc gccgacctca agaaggagct gcagacgacg
     1381 atgcagaaca gcttcggcgt gttccggacc gggactcaca tggaagaggg catcaagaag
     1441 attgcggagc tgcgcgatcg catctctcga gcccatctcg gcgacaagag caaggtcttc
     1501 aacacggcgc ggctagaagc ccttgagctg gacaacctgc tcgaggtggc agaggccacc
     1561 gccattgccg ccaacgcccg cacggagagc cgaggcgctc acgcccggga ggacttccag
     1621 gtgcgcgacg acgagaactg gctttgccac tcgctgtttt tctcggacga aagacgggtt
     1681 accaagcgag ccgtgaactt cagccctgag accgtggaca ccttccagcc catggaacgg
     1741 aagtactaga gaggatggtg atgcaggtta gcgtttatcg atacaacccg gagaccgacg
     1801 aggtcccggc catgcgcgag atgagcgtcg agctccccgg cgaccgggat ctgatggttc
     1861 tcgacgttct cgagatgctg aaggccgagg atcctaccct gtcctaccgg cgctcctgcc
     1921 gggagggtgt ttgcggttcg gacggcatca acatgaacgg gcgaaacggg ctcgcctgca
     1981 tcacgccggt ttcctctgtt gcaaagcggg gcaagctcgt tctgcggccg cttcccgggc
     2041 tgccggtgat ccgggacctg gtggtggaca tgactcagtt ctatcgccag tacgaaaaga
     2101 tcaagccgta cctcattaac gacaagccgc cccctgccca ggagcgcctg cagagtccgg
     2161 aggagcgtgc cgagctcgac gggttgtacg agtgcatcct gtgtgcctgc tgctccaccg
     2221 cctgcccctc gttctggtgg aaccctgaca agttcatcgg tccggcgggt ctgttgcagg
     2281 cctatcggtt cctggccgac agccgggaca cggcgacagc cgagcggctc tcggagctgg
     2341 acgacccctt cagcgtcttc cgatgccgcg ggatcatgaa ctgcgtgagc gtttgcccca
     2401 aggggctcaa ccccacgcgg gccatcggca agatccgcac catgcttctg cagcagggga
     2461 cgtagacgaa ccccttgcgc ggctatactg ctgcttcaac ggacaaaagg ctgacggctc
     2521 tcacggtgag ctcctatccc acgcaggctg acaatggctg actcgtttcg cgagcgcatg
     2581 gagcgtagtt cgcacctggc cggtgccaac gctgcctaca tcgaggccat gtacgagcgc
     2641 tatctcgacg aaccgaacga ggtgccccct gagtggcgcg agtatttcga cacgctgccg
     2701 cgcgtggaag gggctgtcgg tgccgatgtg ccccacagca ccatcgtcga tcagttcgag
     2761 cggctgggac gcaaccgcct gaaagcgaga ccggagcgcg agagccactt tgcgcagaac
     2821 gtgcacgagc agaagcagat gcgcgttcag gatctcatct cggcctatcg gcaccggggc
     2881 cacaagaagg cgaacatcga cccgctgggc atgatggaac gacccgccat gcccatcctc
     2941 gatctggcct atcaccatct ctccctcgcg gatctggacg agacgttcaa cgttggatct
     3001 tttcactacg gatcaccaga ggcgaagctg cgcgatctcg tggatgccct cgagcggacc
     3061 tactgcgcgt ccatcggctt cgagtacatg cacatcgtcg atcagagcga gcagctctgg
     3121 gttcagcagc gcctcgaaag cgtgcgtgcg cggccggtac tgaccgagga tcacaagcgg
     3181 gtgatcctgg aacgcctgac cgctgcagaa ggcctggaaa agtacctgca cggccgttat
     3241 cccggtacca agcgtttcgg cctggaaggc ggggagagcc tcatccccat gcttcacgag
     3301 ctgctgcagc ggctgggttc ccatggcgtc ctcgaggccg tcatcgccat ggcgcatcgg
     3361 ggccggctca acgtgctggt caacatcctg ggaaaagacc cccggctcct tttcgatgag
     3421 ttcgacggca agctgccggt gaacgaagac gacatgggag acgtcaagta ccaccaggga
     3481 ttttccagca acgtgatgac gccgggcggc gagatgcacc tggcgctggc gttcaatcct
     3541 tcccacctgg agatcgtcgg gcccgtggtg gagggatccg tgcgggcgag gcaggatcgc
     3601 cgccgcgacc atgtgggaac ccgggtcgtg cccatcctcg ttcacggcga cgcgtccttc
     3661 gccgggcagg gagtggtcat ggagaccctg cagatgtctc agacccgagg gttcaagacg
     3721 ggcgggacga tccatatcat catcaacaac cagatcggct tcaccaccca tcgtcaggac
     3781 gacgcgcgct ccaccgagta ctgctcggag gtggccaagc tggttcaggc ccccatcttc
     3841 cacgtcaacg gcgacgatcc
//
LOCUS       DBNHQR010000348        10853 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1236660, whole genome shotgun sequence.
ACCESSION   DBNHQR010000348 DBNHQR010000000
VERSION     DBNHQR010000348.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10853)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10853)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10853
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1236660"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="AC16VR_07690"
     CDS             <1..375
                     /locus_tag="AC16VR_07690"
                     /inference="COORDINATES: protein motif:HMM:NF013425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HIR6011076.1"
                     /translation="LFPYTTLFRSRVTAVEHLAEKVAGVAVLKGAGTLIAEHLGSTSR
                     LAGVCGHGNPGMASAGMGDVLSGIIGGLLAQHDVLLSAAVRGVCLHSAAADVVARQVG
                     QRSMLATDLLPEVVSMLAAAEQ"
     gene            372..806
                     /gene="tsaE"
                     /locus_tag="AC16VR_07695"
     CDS             372..806
                     /gene="tsaE"
                     /locus_tag="AC16VR_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HIR6011077.1"
                     /translation="MRPVAVESVFLPDAEATEAFGRDLAVRLEPGDLVLLHGDLGAGK
                     TTLVRGILAGLGHSGSVKSPTYTLLEPYELTSCVVLHFDFYRIGDSRELEFIGFDELM
                     DADAIKLVEWPERAADRLPPPDLEITLRVEDEGRRIEVEAAR"
     gene            784..2094
                     /locus_tag="AC16VR_07700"
     CDS             784..2094
                     /locus_tag="AC16VR_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014819042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HIR6011078.1"
                     /translation="MKSKLLVSAFLLLCYAPPSSAGKLDRVRLHEAPDHTRVVFDVAA
                     PVEFSYFTLRNPDRVVIDLKDTTAGNDFDPSRTGTKSSRVKNIRAAPRGRDYRVVLDV
                     SAPLEPKAFALEPVAAYGHRLVVDLFGAAPKPAAPAAPKADDRRDVIVAIDAGHGGED
                     PGALGPGKVREKDVVLRIARKLEARLNATNGYKAFMVRDGDYYIALRKRVDLARRSRA
                     DLFVSIHADSFRSPEVSGASVYTLSSQGATSETARWMAEKENRSDLIGGVGGVSLDHD
                     DDLLNHVLLDLSTSAKQAASHEAAKSVLGQMARVTKLHKSRVEQAGFAVLKSPDVPSL
                     LVETGYISNPAEANRLASARFQDKIASALFEGVRSYMTRSAPPGTLLAWQRESGPVRY
                     TIERGDTLSDIAVRYGTSTRRLREANGIAGDRIRVGQVIVIPAS"
     gene            2118..3893
                     /gene="mutL"
                     /locus_tag="AC16VR_07705"
     CDS             2118..3893
                     /gene="mutL"
                     /locus_tag="AC16VR_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014853996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HIR6011079.1"
                     /translation="MAGRIQVLEPFVANQIAAGEVVERPASLVKELVENSLDAGARSV
                     RLETELGGMKRVLVSDDGEGIDAADLPLAVARHATSKISGPDDLVGIGSLGFRGEALA
                     SVASVARLTITSATAEAAHGTRLEVDGPGDVHIGPAAHPRGTTVEVRDLFFNTPARRK
                     FLKTERTEQSQVDQVLRRISLCRFDVAFELRHGGTRSVLSLPAGDPELRLARVIGEAF
                     VKRSVALDETRGELRLWGWVGLPTYSRSQADQQYFYVNGRVVRDKLVAHAVRQAYRDV
                     LFGGRQPVFSLYLELPADAVDVNVHPTKHEVRFREARSVHDFIFGTLARALREVRPDA
                     VAAPVAGEAAATGSRGSWTQGRQAGLGLPHAVQDRPFPPAPRSLQEAFAEHGAPITVT
                     ESDQPIPPLGYAVAQLHGVYILAQNADGLVVVDVHAAHERVTYEKLKRQMDTEGVPQQ
                     RLLVPVPVSVSARDADLLEQFEDVVSATGLSLDRSGTQSLMVRAVPALLAGANAEELV
                     RDVLADLAELGSSEQVRNRQHDLLATMACHGSVRANRELTLAEMNALLREMEVTDNAG
                     QCNHGRPTYMVQSMAELDRLFLRGQ"
     gene            3907..4881
                     /gene="miaA"
                     /locus_tag="AC16VR_07710"
     CDS             3907..4881
                     /gene="miaA"
                     /locus_tag="AC16VR_07710"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014860414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HIR6011080.1"
                     /translation="MTVPASGSVALVITGPTAVGKTELAIEIARRVPASLISMDSALV
                     YRGMDVGTAKPSPESLIEHPQALIDVRDPKEPYSAAEFVADADSLVQEAWAAGRVPVV
                     VGGTMMYLRAFREGLADLPQADPQLRRAIAAEAESRGWEALHEQLRAVDPAAAQVIHP
                     NNPQRLQRALEVHRLTGRPISDFWSEQRNADFAARLGGRMETIALMPDRRDRLHARIA
                     RRFAGMLERGLVEEVRQLRARGDLHADLPSMRAVGYRQVWQHLEGELDAAEMRERACA
                     ATRQLAKRQLTWLRGWSWVREVEWGDPVALAEQALLRADQPHGSARRA"
     gene            4993..5241
                     /gene="hfq"
                     /locus_tag="AC16VR_07715"
     CDS             4993..5241
                     /gene="hfq"
                     /locus_tag="AC16VR_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HIR6011081.1"
                     /translation="MSKGHSLQDPYLNTLRKERIPVSVYLVNGIKLQGQIESFDQFVV
                     LLRNSVSQMVYKHAISTVVPSRNVRLSGPGGSEEAAEE"
     gene            5296..6645
                     /gene="hflX"
                     /locus_tag="AC16VR_07720"
     CDS             5296..6645
                     /gene="hflX"
                     /locus_tag="AC16VR_07720"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HIR6011082.1"
                     /translation="MFFERPEAGRRSVLLQVHLKSAAAPDAAVLAESELEAIELARSA
                     GVDVCQVVSARRDNPHPKWFVGPGKVAELAEVLASQEADLLIVNNELNAGQQRDLERD
                     LLCRVLTRTELILHIFADRARTYEGQLQVELAQLRHAQTRLVRGWTHLDRQKGGIGLR
                     GAGETQIEMDQRALGEHIKRVQRKLEQVARRRDQNRRRRRRTRTRTVSLVGYTNAGKS
                     TLFNALTRAGVLVEDKLFATLDPTMRRLTVPGAGDVVLSDTVGFINQLPHDLVDAFKA
                     TLEEVSNADLLLHVVVAGCRDIAERIGQVREVLAEIGASDLPTILVFNKIDQLTPAER
                     RAVLAADVWSAGDGNAMAVAVSAVTGEGLDELTEAIGRRLGVAPEPAKVVLPADDGKT
                     RAWLYRMGAVLGEEITEDGRVALRVRADADLLERLGRTPRVLLQVNDAVPRILPLPN"
     gene            6667..7803
                     /gene="hflK"
                     /locus_tag="AC16VR_07725"
     CDS             6667..7803
                     /gene="hflK"
                     /locus_tag="AC16VR_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003380712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HIR6011083.1"
                     /translation="MAWNEPGGGDKDPWGSRGDQGPPDLDEAFKKLQAQLASIFGGGR
                     GGSGGDGSGLNAGMLGIVLVVAAVAYGLFGLYQLDEQERGVVFRFGAAKEDIVLPGLH
                     WNPPIIDVVRKVNVTRVNSKNHQALMLTEDENIVDVSLTVQYVVNDPLKFLVMVRDPE
                     NSLDHAVESALRHVVGSSLMDDVITAGRAAIAAEVQDRLQTYLDTYGTGILVSKVNID
                     ESAPPNQVRDAFNDVQKAKEDEQRVINEANAYAESIIPEARGEAQKQIEQANAYRDQV
                     IARAEGEAERFEKLLTEYSLAKEVTRDRLYIDAMESVLSRSSKIMIDVEGGNNLLYLP
                     LDRLGQTGSPVAGLATDESVRSIADAILREVNERIASGRIRESR"
     gene            7812..8678
                     /gene="hflC"
                     /locus_tag="AC16VR_07730"
     CDS             7812..8678
                     /gene="hflC"
                     /locus_tag="AC16VR_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019365461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HIR6011084.1"
                     /translation="MNLKNLIIVVIAIIAVLLLMNSVYTVQETERAILLRFGAVSQAD
                     VPPGLHFKMPIAEEVKKADGRVLTLDSVPETYYTLEKKPLIVDSFAKWRIVDVQRYYT
                     GTSFDERRGQRVLQERVNEGLRNQISRRDMHEVVSGERDQLMQELLGDLNAVMSGAVG
                     IEVIDVRVKRIDLPPDVSSSVYDRMNSERQIEARQYRAQGRELALGIRADAERQTVII
                     EAEAYRSSEQIRGDGDARSANIYASAYNRDPEFYQFYRSINAYAKVFKDKSDILVLDP
                     TSEFFKYLANDS"
     gene            8730..9944
                     /locus_tag="AC16VR_07735"
     CDS             8730..9944
                     /locus_tag="AC16VR_07735"
                     /inference="COORDINATES: protein motif:HMM:NF009086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HIR6011085.1"
                     /translation="MVNRKWRLPDGVDELLPPEAWALEQVRRQVLDVFNSWGFDYVEP
                     PVVEYLDALLVGAGPDGGLGHQDDLDLQTLKVVDQRSGRLMGVRADMTAQAVRIDAHS
                     RPSEGVQRLCYAGPVVLANPAAALDSRVPLKAGAEVFGAPGLAADVEVICLLLEILER
                     TGVEAPVLVLGHMGIYLSLVAPLGLDAFTERQLFAAVQSKSEADIGELIGERSDGALL
                     ERLPTLMGGREILREARAALAGGPDGAVAAVDALEELAEAVLVRNPAVEVRYDLAELA
                     GYGYHNGPVFSAYQADQGRAIARGGRYDGIGAPFGRSRPATGFDVNLRQLIGLAGAGT
                     VGQAVWAPWTGSLGGDVAVALAAEVRRLRAAGERVVVALDDEDGAPSGCDRQLSYVSG
                     VWKVERLGRNEG"
     gene            9946..>10853
                     /locus_tag="AC16VR_07740"
     CDS             9946..>10853
                     /locus_tag="AC16VR_07740"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016253964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HIR6011086.1"
                     /translation="MGRNVVVMGTQWGDEGKGKIVDLLTDQVAAVVRFQGGHNAGHTL
                     VIEGEKTVLHLIPSGILRREVQCFIGNGVVLEPNALLSEVSGLEARGVPVRERLRISP
                     ACPLILPYHVKLDLAREEARGNQKIGTTGRGIGPAYEDKAARRGVRLGDLFYWQEFAA
                     KLAEVMEYHNFMLERYYGSETVDYAQTLDECAAVAEQIRPMVADIIPLLHEIRESGAN
                     LLFEGAQGALLDIDLGTYPFVTSSNTTAGGTAVGSGFGPRYLDYILGITKAYSTRVGS
                     GPFPTELFDEVGERLATRGNEFGSTTG"
BASE COUNT         1885 a         3351 c         3751 g         1866 t
ORIGIN      
        1 ctctttccct acacgacgct cttccgatct cgggtgacgg cggtcgagca tctggcggag
       61 aaggttgccg gcgtcgccgt gctcaagggc gccggaacgc ttattgccga acatctcggc
      121 tcgacgagcc gcctggcagg cgtttgcggt cacggcaatc ccgggatggc cagcgcaggc
      181 atgggcgacg tgctgagcgg gatcatcggc gggctgctcg cccagcacga tgtgctcctc
      241 agtgctgccg tcaggggcgt atgtctgcac agcgccgccg ccgatgtggt ggcaagacaa
      301 gtcggtcaac gcagcatgct agcaacggat cttctgccgg aagtggtgtc catgctggcg
      361 gccgccgagc agtgagaccg gtcgcggtgg aatccgtgtt tctgccggac gcggaagcca
      421 cggaggcgtt cgggcgcgac ctggcggtcc ggctcgagcc cggcgatctg gtgctgctgc
      481 atggggacct cggtgctgga aagacaaccc tggtcagggg catactcgcg ggtttaggtc
      541 attccggttc ggtcaagagt cccacgtata ctctgctcga gccttatgaa ttgacgtcgt
      601 gtgtcgtcct gcatttcgat ttttatcgaa taggggacag ccgggaactc gagttcatcg
      661 gatttgacga gctgatggac gcagatgcca tcaagctcgt ggagtggccg gagcgagcgg
      721 ccgatcgcct gcctccgcct gatctcgaga taaccctgcg cgtggaagac gaaggtcgtc
      781 gtattgaagt cgaagctgct cgttagcgca tttctgctcc tttgttacgc gccgccatcc
      841 tcggctggca agctggaccg agtgcgcctg cacgaggccc ccgatcacac ccgcgtggtt
      901 ttcgacgtgg ccgcacctgt cgagttctct tatttcacgt tgcgcaaccc ggaccgggtg
      961 gtcatcgatc tcaaggacac caccgcggga aacgacttcg atccttcgcg gaccgggact
     1021 aaatcgagcc gtgtgaagaa cattcgcgca gcgccgaggg gacgcgacta tcgggtggtg
     1081 ctggacgttt ccgcccccct cgagccgaag gctttcgcgc tcgaaccggt tgccgcgtac
     1141 ggccaccgtc tggtggtcga tcttttcggc gccgcaccga aacccgccgc gccggctgcg
     1201 ccgaaggccg atgaccggcg tgacgtgatc gtcgccatcg atgccggtca cgggggggag
     1261 gatccgggcg cgctcgggcc agggaaggtg cgtgagaagg acgtggtcct gaggatcgcg
     1321 cgcaagctcg aagcgaggct caacgcaacc aacggctaca aggccttcat ggtgcgggac
     1381 ggcgactact acatcgccct tcgcaaacgg gtggacctgg cacgccggtc ccgggccgac
     1441 ctgttcgtgt cgattcacgc ggattcgttc cgcagccccg aagtctcggg cgcttccgtc
     1501 tacaccctct cctcccaggg ggcgaccagc gagaccgcgc gctggatggc ggaaaaggag
     1561 aaccggtccg acctcatcgg cggcgtgggg ggcgtcagcc tggatcacga tgacgatctg
     1621 ctgaaccacg tgctgctcga tctgtccacc agcgccaagc aggcggccag ccacgaggcg
     1681 gccaagtcgg ttctcggcca gatggcccgc gtcaccaagc tccacaagag ccgggtggag
     1741 caggcgggct tcgcggtgct caaatccccc gacgtgccat cgctgctcgt ggagacgggc
     1801 tacatctcga acccggcgga ggccaaccgc ctggccagcg cccggtttca ggacaagatc
     1861 gccagcgcgt tgttcgaggg cgttcgaagc tacatgacgc gctccgctcc gccgggaacg
     1921 ctgcttgcct ggcagcggga aagcgggccg gttcgttaca ccatagagcg gggcgatacc
     1981 ctctccgaca ttgcggtcag gtacggcacc agcacgcggc gcctgaggga agccaacggg
     2041 atcgcgggag accggatccg ggtggggcag gtgatcgtca ttccagcatc gtgagccttc
     2101 tgcgagaatg acgggccatg gccggtcgaa ttcaagtact cgagcccttc gttgccaacc
     2161 agatcgcggc aggcgaggtg gtcgagcgcc cggcttcttt agtcaaggaa ctcgtcgaga
     2221 acagccttga cgccggcgcc aggtcggttc ggctggagac cgagctgggc ggcatgaagc
     2281 gcgtcctcgt cagcgacgac ggcgagggca tcgatgcggc cgacctgccg ctggccgtcg
     2341 ccagacacgc caccagcaag atctcgggac ccgacgacct ggtcggcatc ggcagcctcg
     2401 gtttccgcgg ggaggcgctg gccagcgtgg cgtcggtggc caggctgacg atcacctcgg
     2461 caacggccga ggccgctcac ggaacccgcc tggaagtgga tggcccggga gacgttcaca
     2521 tcggtcccgc cgcccaccca agggggacga cggtggaggt gcgcgatctc tttttcaaca
     2581 ctccggcccg acgcaagttc ctgaagaccg agcgcaccga gcagtcacaa gtagatcagg
     2641 tgctccggcg catcagcctg tgccgtttcg acgtcgcctt cgagctgcgc catggcggca
     2701 cccggtcggt tctgtcgctg ccggcaggag accccgagct gcgacttgcc cgggtaattg
     2761 gcgaggcctt cgtgaagcgc tcggtggccc tggacgagac gcgcggggag ctcaggttgt
     2821 gggggtgggt cgggttgccc acctattccc gcagccaggc ggatcagcag tatttctatg
     2881 tgaacgggcg cgtggtgcga gacaagctcg tcgcccacgc cgttcgccag gcttaccggg
     2941 acgtcctttt cggcggccgg cagccggtct tttcccttta cctggagctg ccggcggacg
     3001 ccgttgacgt gaacgtgcac ccgacgaagc acgaggtccg gtttcgcgag gcgcggtcgg
     3061 ttcacgattt catcttcggt actctggcca gagcgcttcg cgaggtgcgg ccggatgccg
     3121 tggccgcgcc tgttgcgggc gaagcggcgg cgaccggttc ccgggggtcg tggactcagg
     3181 ggcgccaggc cgggctgggt ctgcctcacg cggtccagga tcggcctttt ccgccggccc
     3241 cgcggagcct gcaggaagcg ttcgccgagc acggcgcgcc gatcacggtc acagaatccg
     3301 accagccgat tccgccgctc ggctatgccg tcgctcagct gcacggtgtc tacatcctgg
     3361 ctcagaacgc ggacgggctg gtcgttgtgg acgtgcacgc ggctcacgag cgcgtgacct
     3421 acgaaaagct caagcgacag atggatacgg aaggcgtgcc gcagcaacgt ctgctggttc
     3481 ccgtgccggt gagcgtcagc gcgcgcgatg cggatctgct cgagcagttc gaggacgtcg
     3541 tctcggccac tggtctttcc ttagacagat ccgggacgca gagcctgatg gtgcgcgcgg
     3601 tccccgcgct tctggccggc gccaatgctg aggagctcgt tcgcgacgtc ctggcggacc
     3661 tggccgagct cggttcaagc gagcaggtcc ggaaccggca gcatgatctg ctggctacca
     3721 tggcctgcca cgggtccgtg cgtgcgaacc gtgagctcac tctggctgag atgaacgcgc
     3781 tgctgcggga gatggaagtc acggacaacg ccgggcagtg caatcacggc aggcccacgt
     3841 acatggttca gtccatggcg gagctggacc ggctattcct tcgcgggcag tagacccatc
     3901 gatcccatga cagtaccggc atcgggttcc gtggcgctcg tgatcaccgg gccaacggcg
     3961 gtgggcaaga ccgagcttgc catcgagatc gcccggcgag tgccggccag cctgatcagc
     4021 atggattccg ccctcgttta ccgtggcatg gacgtcggca cggccaagcc ttcaccggag
     4081 agcttgatcg agcaccccca ggctctgatc gacgtccgtg acccgaaaga gccatactcg
     4141 gctgcggagt tcgtcgccga tgccgacagc ctcgttcagg aggcctgggc ggcgggtcgc
     4201 gtgcccgtcg tggtgggcgg caccatgatg tatctgcgag catttcggga aggtctcgcc
     4261 gatctgcccc aggccgatcc gcagctgcgc agggccatcg ctgccgaggc ggagtcccgg
     4321 ggatgggagg ctcttcacga gcagctgcgc gccgtggatc cggccgcggc gcaggtcatt
     4381 catccgaaca acccccaacg cctgcagcgg gcgctggagg ttcaccgcct caccggccgg
     4441 cccatatccg acttctggtc ggagcagcgc aacgccgatt ttgcggcgcg ccttggcggg
     4501 aggatggaga ccattgccct gatgcccgac cgtcgtgacc ggcttcacgc tcggatcgcc
     4561 cgtcgatttg cagggatgct ggagcggggg ctggtggaag aggtgcggca attgcgcgct
     4621 cgcggcgatc ttcacgcgga ccttccgtcc atgcgggcag tgggttatcg ccaggtctgg
     4681 cagcatctcg agggcgagct cgacgcagcc gagatgcgcg agcgtgcgtg cgcggcgacg
     4741 cggcagctcg ccaagcgtca gctcacgtgg ctgcgggggt ggtcgtgggt cagggaggtc
     4801 gagtggggcg acccggttgc gctggcggag caagccctgc tgcgcgcgga ccagccccac
     4861 ggaagtgctc gacgcgcttg aaatatgggc aggctgtagc catagtgaat gtggcggtaa
     4921 aaggacgagc gctgcgtccc cgaaaagtcc tgcaaagccg tcgattgggg ggcgaacttg
     4981 aaggagctgc aaatgtcaaa agggcattcg ttacaagacc cttatctcaa caccctgcgc
     5041 aaggagcgca ttcccgtatc agtttatctc gtcaacggta tcaagctgca gggccagatc
     5101 gagtcgttcg atcagttcgt ggtcctgctg cggaactctg tgagccagat ggtctacaag
     5161 cacgccatct cgaccgttgt gccttcgcgc aacgttcgcc tgtcggggcc cggcggctcc
     5221 gaagaggctg cggaggaata gaccggttca ggccggctct tcccttggcg gaaggcgggc
     5281 ctgaaaggag cacacttgtt ttttgaacga cccgaagcag gccgtagatc ggtcctgttg
     5341 caggttcacc tgaagagcgc tgcggcgccc gatgcagccg tccttgccga atcggagctc
     5401 gaagccatcg agcttgcccg ttccgctgga gtcgacgtct gtcaggtggt ctcggcgcgg
     5461 cgggataacc ctcacccgaa gtggttcgtc ggccccggca aagtcgccga gctcgccgag
     5521 gtgcttgcct cgcaggaagc cgatctcctt atcgtcaaca acgagctgaa cgccggccag
     5581 cagcgcgatc tcgagcgcga tctgctctgc cgggtgctca cgcgtaccga gcttatcctg
     5641 cacatctttg cggatcgtgc ccgaacctac gagggtcagc tgcaggtgga gctggcgcag
     5701 ctcagacatg cccagacccg cctcgtccgg ggttggacgc atctggatcg tcagaagggt
     5761 ggcatcggcc tgcggggtgc gggcgaaacc cagatcgaga tggaccagcg ggcgctgggg
     5821 gagcacatca agcgggttca gcgcaagctg gagcaggtgg cacggcgtcg cgatcagaac
     5881 cggcgccgtc ggcgccgcac ccgcacccgc acggtctcgc tggtgggata cacgaacgcc
     5941 ggcaagtcga ccctgttcaa cgcgctcacc agagccgggg tgctcgtcga ggacaagctg
     6001 ttcgccactc tggatccaac catgcggcga ctgacggttc ccggcgcggg cgacgtcgtg
     6061 ctgagcgaca ccgtcggctt catcaatcag ctccctcacg acctggtcga tgccttcaag
     6121 gcgacgctcg aagaggtcag caatgccgac ctgctgctgc atgtggtggt cgccgggtgc
     6181 agggacatcg ccgagcggat cgggcaggtc cgggaggttc tcgccgagat cggagcgtcc
     6241 gacctgccga cgatcctggt tttcaacaag atcgatcagc tcacgccggc cgagcgccgg
     6301 gcggtgctgg ccgccgatgt ctggtcggcc ggggacggga acgccatggc ggtagcggtc
     6361 agcgcggtca ctggggaggg cctggacgag ctcaccgaag ccatcggaag acgcctcggc
     6421 gtcgccccgg agcccgccaa ggtggttctg cccgccgatg atggcaagac gcgcgcctgg
     6481 ctgtatcgaa tgggcgccgt gcttggcgaa gagatcaccg aggacggacg ggttgcgctg
     6541 cgggttcgtg cggacgccga tctgctggag cgcctgggcc gcacgcccag ggttctgttg
     6601 caggtcaatg acgccgtccc tagaatcttg cccctgccca attaatcttt aacggagcac
     6661 cacgagatgg cctggaacga gccgggtgga ggtgacaagg acccgtgggg aagccgcgga
     6721 gaccagggcc cacctgatct ggatgaagct ttcaagaagc tgcaggccca gcttgcctcc
     6781 atttttgggg gtgggcgcgg cggcagtggt ggcgatggca gcggtttgaa tgcggggatg
     6841 ctgggcattg tgctcgtcgt cgcggccgta gcctacggtc tgttcggcct gtatcagctc
     6901 gacgagcagg agcgaggggt ggtgttccgg ttcggggcgg ccaaggagga catcgtcctc
     6961 ccgggactgc actggaatcc ccccatcatc gacgtggtcc gcaaggtcaa cgtcaccagg
     7021 gtgaactcga agaatcatca ggccctgatg ctgaccgagg acgagaacat cgtcgacgtc
     7081 agcctgaccg ttcagtacgt ggtcaacgac ccgctgaaat ttctggtcat ggtccgcgac
     7141 cccgagaaca gcctcgatca tgccgtggaa agcgcgctgc gccacgtcgt gggaagctcc
     7201 ttgatggacg acgtcatcac cgcgggccgg gcggccatcg cggccgaggt ccaggacagg
     7261 cttcagacct atctggatac ctacggcacg ggcattctcg tgagcaaggt gaacatcgac
     7321 gagagcgcgc cgcccaacca ggtgcgcgac gcgttcaacg acgtccagaa ggccaaggag
     7381 gacgagcagc gcgtcatcaa cgaagccaac gcttacgcgg aaagcatcat tcccgaggcc
     7441 aggggtgaag cgcagaagca gatcgagcag gccaacgcct accgcgatca ggtgattgct
     7501 cgcgccgagg gtgaggcgga gcggttcgag aagctgctca ccgagtacag cctcgccaag
     7561 gaagtgaccc gcgaccggct ctacattgat gccatggagt cggtgctcag ccgatcgagc
     7621 aagatcatga tcgacgtcga gggaggcaac aatctcctgt atctgcctct cgacaggctc
     7681 gggcagaccg ggagcccggt ggccggcctg gccacggacg aatcggtcag aagcatcgca
     7741 gacgccattc ttcgtgaagt caatgaacgc atcgccagcg ggcggattcg ggaaagccga
     7801 taggatccga catgaatctc aagaatctca ttatcgtcgt catagccatc atcgccgtgc
     7861 tgctgctgat gaacagcgtc tacacggtgc aggagaccga acgcgccatc ctgctccgct
     7921 tcggcgcggt cagccaggcg gatgtcccgc ccgggctgca cttcaagatg cccatcgccg
     7981 aggaggtcaa aaaggccgac ggacgcgtgc tgacgctgga ctccgtgcca gaaacctact
     8041 acacgctgga gaagaagccc ctcatcgtcg actccttcgc caagtggcgc atcgtcgacg
     8101 tgcagcgtta ctacacgggc acctcgttcg acgagcggcg cggccagcga gtgctccagg
     8161 agcgggtcaa cgaagggctg cgaaaccaga tcagccgtcg cgacatgcac gaggtggtct
     8221 ccggcgagcg cgaccagctc atgcaggagc tgctggggga cctcaatgcg gtcatgagcg
     8281 gagccgtggg catcgaggtg atcgacgtgc gggtcaagcg catcgacctc cccccggacg
     8341 tctcgagctc cgtgtacgat cgaatgaact cggaacggca gatcgaggcg cgccagtatc
     8401 gcgctcaggg tcgcgagctc gcgctcggca ttcgtgctga cgctgagcgt cagacggtga
     8461 tcatcgaagc cgaggcctac cggagttcgg agcagatacg gggtgacggg gacgcgcgct
     8521 cggctaacat atatgccagt gcgtacaatc gggatcccga gttctaccag ttctatcgca
     8581 gcatcaatgc ctacgcgaag gtgttcaagg acaagagcga catcctggtg ctggatccca
     8641 cgagcgagtt cttcaagtat ctcgccaacg attcctaagc cgggctaaac gttccggctg
     8701 aaatggtccg ctgattggac gtcagatcca tggtcaaccg aaaatggcgc ttgcccgacg
     8761 gggtggacga gctactgccg cctgaggcgt gggcgctgga gcaggtgcgc cgtcaggtgc
     8821 tcgacgtgtt caacagctgg ggcttcgact acgtcgagcc tccggtggtc gagtacctcg
     8881 atgcccttct cgtcggcgca ggtccggatg gggggctcgg tcaccaggac gatctcgatc
     8941 tgcagacgct gaaggtggtc gaccagcgca gcggacgcct gatgggcgtt cgcgcagaca
     9001 tgacggccca ggcggttcgg atcgacgccc acagccgtcc gagcgagggg gtgcagaggc
     9061 tttgctacgc gggccccgtg gttctcgcca acccggcggc ggcgctggat tcccgggttc
     9121 cgctcaaggc aggcgccgag gtgttcggcg cgcccgggct cgcggccgac gtggaagtga
     9181 tctgcctgct gctggaaatt ctcgagcgca cgggcgtcga agccccggtg ctcgtgctcg
     9241 gacacatggg gatctacctg agtctggtcg caccgctggg tctggatgcg ttcaccgagc
     9301 ggcagctgtt cgctgcggtg cagagcaagt ccgaggcaga catcggtgag ctgatcggcg
     9361 agcgctcgga cggggcgctt ttagagcggc tgccgaccct catgggcggc cgcgagatac
     9421 tccgggaggc gcgcgcggcg ttggctggcg ggccggatgg cgcggtggcc gcggtggatg
     9481 ctctggagga gctggcggag gcggtgctgg tccgcaaccc ggcggtggaa gtgcgctacg
     9541 atctggcgga gctcgccggg tacggttatc acaacgggcc ggtgttctcc gcctaccagg
     9601 cggatcaggg ccgcgcgatc gccaggggcg gacgctacga cggaatcggc gcgccgttcg
     9661 gccgcagccg gcccgcgacc ggcttcgacg tgaacctgcg ccagctcatc gggcttgccg
     9721 gggcagggac cgtcggccag gccgtctggg cgccctggac cggcagcctc ggcggcgatg
     9781 tcgctgtcgc gctggctgcg gaggtccggc gcctgcgtgc ggctggagag agggtggtgg
     9841 tggcgctgga cgacgaagac ggggcgcctt cgggctgcga tcgtcagctt tcgtacgtct
     9901 ctggggtatg gaaagtggaa aggctcgggc ggaacgaggg ctgatatggg ccgcaacgta
     9961 gtcgtcatgg gcacccagtg gggggacgag ggcaaaggca agatcgtcga tctcctcacc
    10021 gatcaggtag cggccgtggt ccgtttccag ggtggtcaca acgccggcca caccctggtc
    10081 atagagggcg agaagacggt cctgcacctg attccctcgg gcattctgcg tcgcgaggtg
    10141 cagtgcttca tcggtaacgg cgtcgtcctg gagccgaacg cgctgctcag cgaggtgtcc
    10201 ggtctcgagg cccgtggcgt gcccgtgcgc gagcggctcc ggataagccc cgcctgcccg
    10261 ctcatcctgc cgtaccacgt caagctcgat ctcgcccggg aggaagccag gggtaatcag
    10321 aagatcggca cgaccgggcg cggcataggc cctgcctacg aggacaaagc ggcgcgacgc
    10381 ggcgttcgtc ttggcgatct tttctactgg caggagttcg cggccaagct ggccgaggtc
    10441 atggagtatc acaacttcat gctggagcgg tactacggct cggagaccgt ggactacgcg
    10501 cagaccctgg atgagtgcgc ggcggttgcc gagcagattc gtcccatggt ggcggacatc
    10561 attccgctgc tgcacgagat tcgcgaaagc ggagccaatc tgctgttcga gggagcccag
    10621 ggcgcgctcc tcgacattga tctgggcacg tatccgttcg tgacctcctc caacacgacc
    10681 gcgggcggga ccgcggtggg cagtgggttc ggacctcgat atctggatta catcctgggc
    10741 atcacgaagg catattccac ccgcgtgggc tcgggaccgt ttccgacgga actcttcgat
    10801 gaggttggcg agcggcttgc gacccgcggc aacgagttcg ggtccaccac ggg
//
LOCUS       DBNHQR010000349         5977 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_96122, whole genome shotgun sequence.
ACCESSION   DBNHQR010000349 DBNHQR010000000
VERSION     DBNHQR010000349.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5977)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5977)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5977
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_96122"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(287..547)
                     /locus_tag="AC16VR_07745"
     CDS             complement(287..547)
                     /locus_tag="AC16VR_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011087.1"
                     /translation="MPDVVALSHRGKEFEIRLADDGALELHVDGCLRKRREPTGREPQ
                     YVWTNVELQWEEHHYIEARYWAGNRRLKVTVNGDGIYDRSLP"
     gene            640..2010
                     /locus_tag="AC16VR_07750"
     CDS             640..2010
                     /locus_tag="AC16VR_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR6011088.1"
                     /translation="MPASPDQPRFARVLKSREVITLALGAMIGWSWVLMTGLWVQTAG
                     SLGTLIAFAAGGLAIALIGLTYSELASALPRAGGEHVYTHRALGHRWSFVCTWALLFA
                     YVNVCLFESIALPTAVEYLLPGIRMGTLWSVLGADVDVGFVIVGAGSALLVTVVNVLG
                     IKAAARLQSAAIGLILLAGVILVSGAVVFGETDNARPWIATPATGILSVLIMVPAMLV
                     GFDVIPQSAEEINLQPARIGKLLIVSVLLAVAWYGLIAFAVATALSKPELATSSMATG
                     DAATALWGDPIAGRLLVLGGVAGILTSWNAFVIGGSRVMFALAESGSIPAIFARLHPR
                     YRTPYVGVITIGVMCCFAPLFGRTVLVWLIDAGSFAVVVAYLFVPLAFLALRRQEPDL
                     PRPFKVSHPRLVGVGAFVLAIGLLTAYLPGSPSALIWPYEWAIVLAWTIVGAVLFLAY
                     GAKHPR"
     gene            complement(1975..3543)
                     /locus_tag="AC16VR_07755"
     CDS             complement(1975..3543)
                     /locus_tag="AC16VR_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012686.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HIR6011089.1"
                     /translation="MDLIGWSWLFLVLYLALMLAFGFIGRRRIKSADDFATARESYGP
                     VFLAFAFAATTASGATFVGFPGIAYDAGLPAVWSVFLYPAGVYLGVLICLKVVSNTGH
                     EFGSRSIPEYLGTRYQSDWMRVLVSLFSLLLFFYLAGQLVSGIVMFEIMLGVSPNWAL
                     AITAAVLMVYVVLGGAHADILTDGVQGLIMVAIAVGLVFLFVSGFGVDGGFTGMIDSL
                     ERQDANLVGWLNPDYVLYHSWWSVAAVLLAHIPLGMLPHIGNKLWALEQPSQRRRFIR
                     LAFTFGLALGMLGLGGLLARAILGDALLQPGESSNTALSMLFVELFPGWLAALLGIGI
                     LAAVMSTADGLVVSSSQIIANDLYRCTIVPRFARELPERTVDRQVLIISRIATVAVMA
                     ICTAMAWALADRNVALIVWIGTGGMMAAFAGPLVVGAVWRGVTKAGAFAGLVGGVTVF
                     SIAHGGLVDPGWFEPGALRDAAAWLLREAPNPWSCAAMGEIVSIALTWGVSKLTQPLP
                     SDHLTRMFGPVGQE"
     gene            complement(3576..4694)
                     /locus_tag="AC16VR_07760"
     CDS             complement(3576..4694)
                     /locus_tag="AC16VR_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HIR6011090.1"
                     /translation="MAERNRAQNPPPPSRSDNGWLKGRGATKNEASRYLARHSVPVDD
                     GWSANEVDEPDGSRVATELYPDRTVTLITRNASPDIPFDRSINPYKGCEHGCVYCFAR
                     PTHAYLDLSPGLDFETKIFFKTNVRERLVDELGKRRYQCRTIAMGTNTDPYQPAERHR
                     RITRSVLETLLEARHPVSIVTKSQLILRDLDLLQELARQQLATVAVSLTTLSNDLKTR
                     LEPRTAGPGARLRVIRELRGAGVPVGVMVAPVIPFVNDHEIEDIVAAATDAGARTANY
                     ILLRLPLEVSGLFEAWLGVHFPDRAERVMNAIRSARGGKTYDATWGKRMRGEGEVAEL
                     IAARFKVALRRNGILGATQPPLRTDLFRPPGGSQLSLI"
     gene            4800..5627
                     /locus_tag="AC16VR_07765"
     CDS             4800..5627
                     /locus_tag="AC16VR_07765"
                     /inference="COORDINATES: protein motif:HMM:NF017434.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc protease family protein"
                     /protein_id="HIR6011091.1"
                     /translation="MNRPRTAFEEVRRGAMAHRAGPGRALLRAPLLALLMSGCAVTTG
                     SQDNPNAALVSRVQALEYQVLATQVAVRELAEDQQSGRADLSRQMGDLNTELGRVPEA
                     VGAICASAETAAAAACDSDQPIQTVVMSGDKMVVGEMERVWLEPPGTTLIARVDTGAH
                     SSSLHAENLVEFERDGEEWVRFDLILDDEIKTLERRVVRYVRVYQQADPEGTRRPVVT
                     MRLRIGDVHDSFEFTLADRSHLDFQLLLGRNFLADMALVDVGKQFVQPPYQPPSTSR"
     gene            5624..>5977
                     /locus_tag="AC16VR_07770"
     CDS             5624..>5977
                     /locus_tag="AC16VR_07770"
                     /inference="COORDINATES: protein motif:HMM:NF025758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UUP1 family membrane protein"
                     /protein_id="HIR6011092.1"
                     /translation="MSQKGPFLFLVALLCIAGVGTAAFRHAEYRIPWLPGEQQNVWEI
                     EARIEYMAQGVPTQAFLTLPPNQDGFRVVNETGASSGFGFEVETQRRQRRAHWSKREA
                     NGPQTLFFKLELVRDA"
BASE COUNT         1014 a         2003 c         1932 g         1028 t
ORIGIN      
        1 gccgaagtcc gccgtcatgg cctctaccag ccgcttctcg tgcgtcttga ggagacccac
       61 agcccgctcg aggcgatcca cgcgcttggc caacgaaacg gttccttccc caaggtaatc
      121 ctctcgctgg cgctcgagaa gcgccagcat ctgctcaagg gtcatgtcct gcatgtctgc
      181 tcccagttcg taatctcggc gtttcgagga ccggccatca tactcgcctt caatctcgat
      241 ctcgacgcgc ctcatcggcc cgtctgcagt aggcggcgct catccctcaa ggcagcgatc
      301 ggtcgtaaat cccgtcacca ttgaccgtca ccttcaggcg tcggttccct gcccagtaac
      361 gggcctctat gtaatggtgc tcctcccact ggagctcgac gttggtccag acgtactgag
      421 gctcccggcc cgtcggctcg cgacgcttgc gcaggcaccc gtcgacatgg agttccaggg
      481 cgccgtcgtc cgccaggcgg atctcgaact ccttgccgcg atggctcaat gcgacgacgt
      541 ctggcaaacc aggctccagg tgttgaaatc gaaaggtcga gggaggcggc aattaccgct
      601 aagcttgcgg gctcgaattc tgcccgcagg cgccacgcga tgccagcaag ccctgaccaa
      661 ccccgctttg cccgagtgct gaagagccgg gaagtcatca ccctggcgct gggcgccatg
      721 atcggctgga gctgggttct gatgaccggg ctctgggtgc agacagccgg cagcctgggc
      781 acgctcatcg ccttcgccgc gggcggtctg gccatcgcgc tgatcgggct gacctacagc
      841 gagctcgcct ccgccttgcc gcgcgcgggc ggcgagcacg tctacaccca tcgcgcgctg
      901 ggacaccgct ggtcgttcgt ttgcacctgg gccctcctgt ttgcctacgt gaacgtgtgc
      961 ctgttcgaat ccatcgcttt gcccacggcg gtggagtatc tgctgcccgg tattcgcatg
     1021 ggtaccctct ggagcgttct cggtgcggac gttgacgtcg gcttcgtgat cgtgggcgcg
     1081 ggcagcgcgc ttctggtcac cgtggtcaac gtgctgggca tcaaggccgc cgcgcggctg
     1141 cagagcgcgg ccatcgggct gatcctgctg gccggcgtga ttctcgtcag cggtgcggtg
     1201 gtcttcgggg agaccgacaa tgccaggcct tggatcgcca cgccggccac cgggattctc
     1261 agcgtgctga tcatggtgcc cgccatgctg gtgggctttg acgtgattcc ccagtccgcc
     1321 gaggaaatca acctccaacc cgcccgcatc ggcaagctgc tcatcgtatc cgtgctcctc
     1381 gcagtagcct ggtacgggct gatcgccttc gccgtcgcca cggcgctaag caaacccgag
     1441 ctggccacga gctccatggc cacgggcgac gccgccaccg ctctctgggg tgaccccatc
     1501 gccgggcgcc tcctcgttct cggcggcgtg gccggcatcc tcaccagctg gaacgccttc
     1561 gtgatcgggg gcagccgcgt gatgttcgcg ctggcggagt caggctccat cccggccata
     1621 ttcgcccggc tgcatccccg ctaccggacg ccctacgtcg gggtcatcac catcggcgtg
     1681 atgtgctgct ttgcgcctct attcggacgc accgtgctgg tctggctcat cgatgcgggg
     1741 agcttcgccg tggtggtggc ttacctgttc gtgcccctgg cctttctggc cctgcgacgc
     1801 caggagcccg acctgccccg gccgttcaag gtctcccatc cgcgcctggt gggtgtcggc
     1861 gccttcgtgc tcgccatcgg cctgctcacc gcctacctgc ccggcagccc gtctgcgctc
     1921 atctggccct acgagtgggc catcgtgctc gcctggacca ttgtcggcgc ggtgctattc
     1981 ctggcctacg gggccaaaca tccgcgttag atgatctgac ggcaggggtt gggtgagctt
     2041 ggacacgccc caggtcagcg cgatggacac gatctccccc atggccgcgc aggaccaggg
     2101 gttgggcgct tcccggagaa gccaggcggc ggcatcgcgc agcgccccgg gctcgaacca
     2161 gccggggtcg acgaggccgc cgtgggcaat ggagaaaacg gttaccccgc ccaccagacc
     2221 ggcgaacgcg cccgccttgg tcacgccgcg ccagacggcc ccaaccacca gcgggcccgc
     2281 gaatgccgcc atcatgcccc ccgttccgat ccacacgatc agggcaacgt ttctgtcggc
     2341 cagggcccag gccatggcgg tgcagatggc cattaccgcc acggtcgcta tgcggctgat
     2401 gatgagaacc tgcctgtcca cagtgcgctc gggcagctcg cgcgcaaacc ggggaacgat
     2461 ggtgcagcgg taaaggtcgt tggcgatgat ctgcgacgac gaaaccacca ggccgtcggc
     2521 cgtggacatc accgccgcga ggatgccgat acccagcagg gcggcgagcc atcccggaaa
     2581 aagctccacg aagagcatgg acagcgccgt gttgctcgac tcgccgggct gcagcagggc
     2641 gtctcccaga attgcccgcg cgagcaggcc gcccagcccc agcatcccca gcgcgaggcc
     2701 gaaggtgaat gccagccgga tgaatcggcg gcgctggctg ggctgctcca gcgcccacag
     2761 cttgttgccg atatgcggca gcatgcccaa ggggatgtgg gcgagcagca ccgcagcgac
     2821 agaccaccag gagtgataga ggacgtagtc cgggttcagc cagcccacca ggttcgcatc
     2881 ctggcgctcg aggctgtcaa tcatgcccgt gaagccgccg tcgactccga agcccgagac
     2941 gaagaggaag acgagcccca ccgcgatggc caccatgatc agtccctgca ccccgtccgt
     3001 gaggatgtcg gcatgagccc cgcccagcac gacgtagacc atcagcaccg ccgcggtgat
     3061 cgccagcgcc cagttcgggg agacgccgag catgatctcg aacatcacga tcccggagac
     3121 cagctgacct gccaggtaga agaagagcag cagggaaaat agagagacca gaacccgcat
     3181 ccagtccgac tggtatcggg tgccgaggta ctccggaatg cttctgctgc cgaactcgtg
     3241 cccggtgttg ctgacgacct tgaggcagat caggaccccg aggtagacgc ctgcggggta
     3301 aagaaagacg gaccatacgg caggcagccc cgcgtcatag gcgatacccg ggaagccgac
     3361 gaaggtcgcg ccgcttgccg tggtggccgc aaaggcgaaa gcgagaaaca cgggcccgta
     3421 ggattcccgc gcggtcgcga agtcgtcggc gcttttgatc cgccttctgc cgatgaagcc
     3481 gaaggcgagc atcagcgcca ggtagagcac cagaaataac catgaccagc cgatcagatc
     3541 catgctcacc ctccccgccg agcggttaaa gcttatcaga tcaggctcag ctggctgcct
     3601 ccggggggtc ggaagagatc tgtgcgaaga ggcggctggg tggcgccgag aatgccattg
     3661 cgcctgagcg cgaccttgaa gcgcgcggcg atgagctcgg caacttctcc ctcgccccgc
     3721 attcgcttgc cccacgtggc gtcgtaggtc ttgccgcctc gggccgagcg tatcgcgttc
     3781 atgacacgct cggcgcgatc cggaaagtgc acgcccagcc aggcttcgaa gaggccgctc
     3841 acctccagcg gcaggcgcag caggatgtaa ttcgccgtgc gagcaccggc atcggtagcc
     3901 gcggccacga tgtcctcgat ctcgtgatcg ttaacaaagg ggatgacggg cgccaccatg
     3961 accccgacgg gaacgcctgc gccccgcagc tccctgatga ccctgagcct ggccccaggt
     4021 cccgccgtgc gcggctcaag gcgggtcttc agatcgttgc tcagcgtcgt gaggctcacc
     4081 gccacggttg ccagctgttg gcgagccagt tcctgaagga ggtcgaggtc gcgcagtatg
     4141 agctggctct tggtcacgat gctcaccgga tgccgggctt ccagcagcgt ctccagcacg
     4201 ctccgggtga tccgccggtg ccgctcggcg ggctggtacg gatccgtgtt ggtccccatg
     4261 gcgatcgtgc ggcactggta gcggcgcttt cccagctcgt cgacgagccg ctcgcggacg
     4321 ttcgtcttga agaagatctt cgtctcgaaa tccagccccg gcgagagatc caggtaggcg
     4381 tgagtgggcc gggcgaagca atagacgcag ccgtgctcac agcccttgta gggattgatg
     4441 gaacggtcga aggggatgtc cggcgaggcg ttgcgggtga tcagcgttac tgttctgtcc
     4501 ggatagagct ccgtggccac cctggacccg tctggctcat cgacttcgtt ggccgaccag
     4561 ccgtcgtcca cgggcacgct gtgccgggcg agatagcggc tcgcctcgtt tttggtagcg
     4621 ccgcggccct tcaaccagcc gttgtccgac cggctgggcg gcggcggatt ctgcgcgcga
     4681 tttctctctg ccattgaagg gccttccaac agacaggcag aataactgta tatatgtaca
     4741 gtatcaagcg tcggataccg acagtagact tcgacgccga agcccaaact gacctgcaga
     4801 tgaaccgacc gagaacggcc tttgaagagg ttcggcgcgg cgctatggct caccgcgccg
     4861 gtccgggccg cgccctgctg cgggcgccgc tgctggcgct gctgatgagc ggatgcgcag
     4921 tcaccaccgg cagccaggat aatcccaacg cggcgctggt cagccgcgtg caggcgctgg
     4981 agtaccaggt gctggcgacc caggtcgcgg tcagggagct ggccgaggat cagcagtcgg
     5041 gccgggccga tctctccagg cagatgggcg acctcaacac tgagcttggc cgcgtgcccg
     5101 aggccgtcgg cgcgatctgc gccagcgcgg agaccgccgc ggcggcggcc tgcgattccg
     5161 accagcccat ccagaccgtg gtcatgagcg gcgacaagat ggtggtcggc gagatggagc
     5221 gggtttggct ggagccgccc ggcacgaccc tcatcgcccg tgtcgacacc ggtgcccatt
     5281 ccagctccct gcatgcagag aacctggtgg aattcgagcg cgacggcgag gaatgggtgc
     5341 gcttcgacct gattctggac gacgagatca agactctgga acggcgggtg gtccgctacg
     5401 ttcgcgtcta tcagcaggcc gatcccgaag gcacccgccg accggtggtc accatgcgct
     5461 tgcgtatcgg cgacgtgcac gactccttcg agttcaccct ggcggaccgg tcgcacctag
     5521 attttcagct gctgctggga cgtaacttcc tcgccgacat ggcgctggtg gacgtcggca
     5581 agcagttcgt ccagccgccc taccagcccc cgtcaacctc cagatgagcc aaaaggggcc
     5641 atttctcttc ctggtggccc tcctgtgcat cgccggcgtc ggcacggccg cgttccggca
     5701 cgccgagtac cggataccct ggctgcctgg cgagcagcag aacgtctggg agattgaggc
     5761 gcgcatcgag tacatggccc agggcgtccc gacccaggcc tttctgacat tgccccccaa
     5821 tcaggacggc ttcagggtgg tcaacgaaac cggcgcatcg tcaggcttcg gcttcgaggt
     5881 agagacccag cgccggcagc gcagggcaca ctggtccaag cgggaggcga acgggccgca
     5941 aacccttttc ttcaagctgg agctcgtgcg cgatgcg
//
LOCUS       DBNHQR010000350         2724 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_412519, whole genome shotgun sequence.
ACCESSION   DBNHQR010000350 DBNHQR010000000
VERSION     DBNHQR010000350.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_412519"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="AC16VR_07775"
     CDS             <1..488
                     /locus_tag="AC16VR_07775"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR6011093.1"
                     /translation="LVGVLLLSNRVRLQDIFQADRSVLLGLASGAAFAVAIVSFRGAS
                     LALPSGDYLIRAALTLAVAVSLQSLIMGAYLGRFETGELRRVVRAWRPASLVGLTGML
                     ASAGWLTAVTLESAALVRAVGQVELLFTFAASIWFFGERVGWREAVGVALVVIGIWVL
                     L"
     gene            complement(485..1054)
                     /gene="idi"
                     /locus_tag="AC16VR_07780"
     CDS             complement(485..1054)
                     /gene="idi"
                     /locus_tag="AC16VR_07780"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018402749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HIR6011094.1"
                     /translation="MSHPATKSEIVSSEEELLILVDGDDREVGFLEKSACHDGAGVLH
                     RAFSLFVLNSAGEVLIQQRASGKRLWPDYWSNSCCSHPRRGETMHEAARRRLHQELGM
                     TAPLEFLYKFEDQADYRGLGAEHELCSVYVGHSDATPVINTTEIGDWRWSARDDLKRE
                     LDTRPEQFTPWFLMEWERILRDHPAALHA"
     gene            1184..2071
                     /locus_tag="AC16VR_07785"
     CDS             1184..2071
                     /locus_tag="AC16VR_07785"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6011095.1"
                     /translation="MSAYGHPREHRFQGSQVELAWYEWGEAPHPKVLLVHATGFHARC
                     WDQVVAHLPPGFHILAVDLRGHGRSTKRGPYVWSSFLADLEEFAAALSLAGAVGVGHS
                     LGGHCITQLAARRPGAFDRLVLVDPVILDPEAYARPRYADLQSAEDHPVARRRDHWAG
                     WEEMFERFSSRKPFSRWRPQVLEDYCRHGLEPRAEGGWRLACPPVVEASIYLGNKGSD
                     VYGMLHDVDVPVAVLRAPGRDPDDHDIMDFSKSPTWPELADRFPRGRDVFLPRLDHFI
                     PMQAPELVARFITDGDADP"
     gene            2068..2322
                     /locus_tag="AC16VR_07790"
     CDS             2068..2322
                     /locus_tag="AC16VR_07790"
                     /inference="COORDINATES: protein motif:HMM:NF016141.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HIR6011096.1"
                     /translation="MNFLSWLLLGLVVGTLAKLIMPGPDPGGFFVTIGIGMAGAFIGG
                     YGAHLLGIGDVAGFDLASLGLATAGAVLLLGTYRVVRGGF"
     gene            complement(2295..>2724)
                     /locus_tag="AC16VR_07795"
     CDS             complement(2295..>2724)
                     /locus_tag="AC16VR_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011097.1"
                     /translation="MPLKHPGRLYLLLQLTRMFILGLLWLQPSLWLFYVLIVAWGLNM
                     GVTTTLVRTTVQELAPEHHRAQILSVLLLSFVVSSPISSILLGVLIAETEPLTAMLPG
                     IAISLLIFLIGTTRSGLWGYVSPATKTRAAGPQNPPRTTR"
BASE COUNT          394 a          862 c          939 g          529 t
ORIGIN      
        1 gcctggttgg tgtgctgctt ctttccaacc gcgtgcgcct ccaggacatt tttcaggctg
       61 atcgctccgt gctcctgggg ctcgccagcg gggccgcgtt tgcggttgcc atcgtcagct
      121 ttcgcggagc gtcgctcgcg ctgccctccg gagactacct gatcagggcg gcccttaccc
      181 tggcggtggc cgtctctctg cagtcgctca tcatgggcgc gtacctcggc cggttcgaga
      241 cgggagagct gcgacgcgtc gtccgggcct ggcgccctgc gtctctcgtg gggctcacgg
      301 ggatgctcgc gtcggccgga tggctcacgg cggtgacttt agagagcgcg gcgttggtgc
      361 gggccgtggg gcaggtagag ctgcttttca cgttcgcggc ctccatctgg ttcttcggcg
      421 aacgggtcgg ctggcgcgaa gcggtgggcg tcgccctggt cgtgatcggt atctgggtgt
      481 tgctctaggc gtgcagggcc gcagggtggt cgcgcaggat tcgttcccac tccatgagga
      541 accagggcgt gaactgctcc ggccgcgtgt cgagctcccg tttcaggtcg tcccgggcgc
      601 tccagcgcca gtcgccgatc tcggtggtgt tgatgacggg tgttgcgtcg ctgtggccga
      661 cgtagacgga gcagagctcg tgctcggcgc ccagcccgcg gtagtccgcc tggtcttcga
      721 acttgtagag gaactcgagc ggcgcggtca tgccgagctc ctgatgcagc ctgcggcgcg
      781 cggcctcgtg catggtctct ccgcgccgtg gatggctgca gcagctgttg gaccagtagt
      841 cgggccacag ccgcttgccg gaggcccgct gctggatgag cacctctcca gcggagttga
      901 gcacgaacag ggagaacgcc ctgtgcagca cgccggcccc gtcgtggcag gcggactttt
      961 ccagaaaacc gacctcacga tcatcgccgt ccacgaggat cagcagctcc tcctctgagg
     1021 agacgatctc ggattttgtt gctgggtgag acatgaagac cggctcggcg tgagaaagat
     1081 cagtgtagcg gaattggcga cggcggtctg cgtgtcttgt ctggcctgcc cgctctccgt
     1141 agcatgatca cgttgcggcc atcggccgcg cgatcagttt ttcatgagcg catacggaca
     1201 tcccagggag caccgttttc agggctctca ggtagagctt gcctggtacg aatggggcga
     1261 ggcgccgcat cccaaggtgc tgctcgtgca tgccaccggc ttccacgccc gctgctggga
     1321 tcaggtggtc gctcatctcc ccccgggttt tcacatcctc gccgtggacc tgcgcggcca
     1381 tggccgcagc accaaacggg gtccttacgt gtggagcagt ttcctggcag acctggaaga
     1441 gtttgccgcc gcgctgagcc tggcgggtgc cgtcggcgtc gggcactctc tgggcggtca
     1501 ctgcatcacg cagctggcgg cgcggcgtcc gggcgcattc gaccggctgg ttctggtcga
     1561 ccccgtcatc ctggatccgg aagcttacgc gcgcccgcga tatgccgacc tgcagtcggc
     1621 ggaggatcac cccgttgccc ggcgtcggga tcactgggcc ggctgggaag agatgttcga
     1681 aaggttctct tcgcgcaagc cgttctcgcg atggaggccg caggtgctgg aggactactg
     1741 ccgccacggc ctcgagcccc gtgcggaagg cggctggcgt ctggcctgcc cgcccgtggt
     1801 ggaagcttcg atttacctcg gaaacaaggg cagcgacgtc tacggcatgc tgcacgatgt
     1861 cgacgtgccg gtagccgtgc tgcgagcgcc cgggcgcgac cccgatgacc acgacatcat
     1921 ggatttctcc aagtcgccga cctggcccga gctggcggac aggtttccgc gggggcggga
     1981 cgtctttctg ccgcggctcg atcacttcat tcccatgcag gcgccggaac tggtggcccg
     2041 cttcatcacc gacggagatg cagacccgtg aacttcctat cctggctcct tctcgggctc
     2101 gtcgttggca ccctcgccaa gctgatcatg cctggccccg atccgggcgg ctttttcgtc
     2161 accatcggca tcgggatggc gggcgcgttc atcgggggtt acggcgccca cctgctgggg
     2221 atcggcgacg tggccggatt cgatctggct agcctcggcc ttgccaccgc cggcgcggtg
     2281 ctgctcctgg ggacctaccg ggtcgttcgc ggcgggttct gaggccctgc ggctcgagtc
     2341 ttggtcgcgg gggaaacgta gccccacagc ccgctgcgcg tggtcccgat caggaagatg
     2401 agcagagaga tggcgatgcc gggaagcatc gcggtcagcg gctcggtctc ggcgatcaac
     2461 acgcccagca ggatcgagct gatgggcgag gacaccacga agctcaagag cagcacggag
     2521 aggatctgcg ctctgtgatg ttccggcgcc agctcctgca ccgtggttct gaccagggtg
     2581 gtggtgacgc ccatgttcag gccccaggcc acgatcagca cgtagaacag ccacaggctc
     2641 ggttgcagcc ataaaaggcc caggatgaac atccgggtta gctgcagtag caggtagagc
     2701 cttcccggat gcttcagcgg catg
//
LOCUS       DBNHQR010000351         2072 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_96351, whole genome shotgun sequence.
ACCESSION   DBNHQR010000351 DBNHQR010000000
VERSION     DBNHQR010000351.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_96351"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="AC16VR_07800"
     CDS             <1..925
                     /locus_tag="AC16VR_07800"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6011098.1"
                     /translation="AIGGMNVTATVEGLERYPVNVRYPRELRDTPDALRRVLIATPTG
                     VQIPLEAVADISMNRGAPVIKSENGRPNAWIYVDPRTSDIGGFVAKAQRLVAQQVAIP
                     AGYTIRWSGQYEYMERARTRLQILVPVTLLAIFLLLYGHFRNVLAPAVVMLSIPFGLI
                     GGVWLTIALGYNLSVAVAVGFIALAGMAAEIGVLVLSFIDAEIARLRGDGGRLSAAAI
                     REAARSATAQRVRPVVMTAASTMAGLLPIMLSSGTGSDVTHRIAAPMLGGMLTVMILN
                     LLVLPVIYSLALQHREKRLGSEGTRQSESSA"
     gene            1063..>2072
                     /locus_tag="AC16VR_07805"
     CDS             1063..>2072
                     /locus_tag="AC16VR_07805"
                     /inference="COORDINATES: protein motif:HMM:NF012398.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR6011099.1"
                     /translation="MQTYDKFYINGEWVAPHGTGTIEVIDPSTEEVCGVVPSGDDVDV
                     DAAVAAAKAAFKTWSRSSAAERSEIIGKLADKITENMAKIGELCARELGTPLQASIAT
                     HGGLGIGVMSSYKEIPFEMEKEEQIGNSIVVKEPIGVCAFITPWNFPLHQIVAKLAPA
                     LAAGCTVVVKPSSDTPLTAYFLAEMIEEIGVPAGVFNLITGPGRTVGEAMCTHPDVDL
                     VSITGSTEAGIRVAQLAAGTVKRVCQELGGKSPLVALEDADISATAARAAGGICFNSG
                     QVCAALSRLIVPRGKQDEAIAAAKAAAESVVVGGAFEEGVTMGPVASKSQFDSVRAYI
                     EKG"
BASE COUNT          358 a          639 c          712 g          363 t
ORIGIN      
        1 cgcaatagga ggcatgaacg tcacggcgac ggtggagggc ctggagcgct acccggtgaa
       61 cgtgcgttac cctcgggagc tccgcgatac gccggacgcg ctgcgccggg tgctcatcgc
      121 cacgcccacc ggggtgcaga tcccgctcga ggcggtggcg gatatctcca tgaaccgggg
      181 cgcgccggtg atcaagagcg aaaatggacg tcccaacgcc tggatttacg tggacccgag
      241 gacctccgac atcggcggct tcgtcgccaa ggcccaacgg ctggtagccc agcaggtcgc
      301 gatcccagcc ggctacacca tccgctggtc cggacagtac gagtacatgg agcgagcgcg
      361 tacccgcctg cagattcttg tcccggtgac cctgctggcc atttttctcc ttctctacgg
      421 tcacttccgc aacgtgctcg caccggccgt ggtgatgctt tccatcccgt tcggactcat
      481 cggcggggtg tggctgacaa tcgccctcgg ctacaaccta tcggttgccg tcgctgtggg
      541 cttcatcgcc ctggcaggca tggccgccga gatcggggtt ctggtgctga gcttcatcga
      601 cgcggagatc gcgcgtctgc gcggtgatgg cggccgcctg tcggctgccg cgatccggga
      661 ggcggcccgg tccgccacgg cacagcgggt gcgtccggtg gtgatgacgg cggcctcgac
      721 catggccggc ctgctaccca tcatgctcag cagcgggacg gggtcggacg tgactcatcg
      781 catcgcggcg cccatgctcg gaggcatgtt gaccgtgatg attttgaatc tcctggtgtt
      841 gccggtgatc tacagcctgg ctctgcaaca tcgcgaaaag cgcctcggat ccgagggaac
      901 acggcagtcg gagtcgtcgg cgtagctgtt tacaaccgcc atggcatcat gttgagatca
      961 ggggtgcggc ggcaatctgg ggccgccgaa ggttcggggg aatcgggggc tttcaatcca
     1021 cggcccatgc tgagcactgc agataccaag taggagatga atatgcagac ttacgacaag
     1081 ttttacatca acggtgagtg ggtcgcgcct cacgggaccg ggaccatcga ggtcatcgat
     1141 ccgtccacgg aggaagtctg cggcgtcgtg ccgtcgggcg acgacgtcga cgtcgacgcg
     1201 gcggtggccg cggcaaaggc ggctttcaag acctggtcgc gatcgtcggc cgccgagcgt
     1261 tccgagatca tcgggaagct ggcggacaag attaccgaga acatggccaa gatcggcgag
     1321 ctctgcgccc gtgagctggg caccccgctg caggcgagca tcgccaccca cggtgggctg
     1381 ggcatcgggg tgatgagctc gtacaaggaa atacccttcg agatggagaa ggaggagcag
     1441 atcggcaact ccatcgtcgt caaggaaccc atcggggttt gcgccttcat caccccgtgg
     1501 aacttcccgc ttcatcagat cgtcgccaag ctggcgccgg ctctggcggc cggctgcacc
     1561 gtggtggtca agcccagttc cgacacgccg ctgacggcct acttcctggc ggagatgatc
     1621 gaggaaatcg gcgtgccggc cggtgtgttc aacctgatca cgggtcccgg caggacggtc
     1681 ggagaggcca tgtgcacgca tccggacgtg gacctggtct caatcacggg atccacggaa
     1741 gccggcattc gggtcgccca gctcgcagcg ggaaccgtca agcgcgtgtg ccaggaactt
     1801 ggtggcaagt cgcccctggt cgccctggag gatgcggaca tctcggctac cgccgcccgg
     1861 gcggctggtg gcatctgctt caacagcggg caggtctgcg ccgcgctcag ccggctcatc
     1921 gtgccccgtg ggaagcagga tgaagccatc gcggcggcca aggcggcagc ggaatccgtg
     1981 gtggtcggtg gcgctttcga ggaaggggtg accatggggc ccgtggcttc caagtcgcag
     2041 ttcgactccg tgcgggcgta catcgaaaag gg
//
LOCUS       DBNHQR010000352         3788 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_856544, whole genome shotgun sequence.
ACCESSION   DBNHQR010000352 DBNHQR010000000
VERSION     DBNHQR010000352.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3788)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_856544"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(253..1671)
                     /locus_tag="AC16VR_07810"
     CDS             complement(253..1671)
                     /locus_tag="AC16VR_07810"
                     /inference="COORDINATES: protein motif:HMM:NF012398.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR6011100.1"
                     /translation="MAEQAAHQELTPQEEIRGLIERSRAAQAQIENYTQEQVDDLIRA
                     MVWCVAKPGVAEEIAQFTVDETQLGNYDGKYLKIHRKTRATLMDIINDKSVGILEEDA
                     ERNIVKIAKPMGVIGALAPSTNPEATPVIKAIHAVKGRNSIIVAPHPRAKLTNKMICD
                     KMREAIAACGAPADLVIAIESPSLELTNELMRQCDRVLATGGGAMVHAAYSSGTPALG
                     VGVGNAVITVDETADLEDAAEKIRLSKTLDLAASCSADNSVILVDAIYDEMMNKLEHQ
                     GGYLCNAEEKAKLQSTLWDDEGHLNTAIVAQPAEKIAAMAGIELPEGRSFYIVPEDGW
                     GPEHPFSGEKLSVIMALYRARDIDHAIELTNNIQSYQGQGHSCGIYSTSDDNIMKLAN
                     ATKTSRVMVNQPQAASNSGNLWNGMRQTFSLGCGSWGGNGTNHNISWRDLINETWVSK
                     PLEKTKELPSDEELFGDVMGRI"
     gene            complement(1766..3385)
                     /locus_tag="AC16VR_07815"
     CDS             complement(1766..3385)
                     /locus_tag="AC16VR_07815"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR6011101.1"
                     /translation="MSSHRIEVTTLGDLLLRAADRHGDRDAIIFPEARLTYDQLREGA
                     YARARSLLALGVDRGDHVGILMPNCLDYVECLFAVQLLGAMAVPINARYKARELAYVV
                     ENADLSAILISDVISEYADFAELLQEAFPELSANADPRALELPGAPKLKTVVMLGSSA
                     PAGILPRAELDALASGYQVEDVEARRVCARVRDQAIMMYTSGTTSDPKGCPLSHEVLV
                     RNGFNMNRERYFLDETDCFWAPLPMFHMASILPMICCMDAGAALQSMTHVEAGGALAM
                     MERDATTVAFPSFPTVTNELINHPEFTTRDLSRIRRINNVAPPDVLRKFQDAFPQAVQ
                     TGAYGLTEVGGVIAFNHPDESLEQRLTTCGRPFPGVEVKVVDPDTGAELGPGERGEIL
                     VRGYAVFDGYYKAPEKNAEAFADGWFRTGDLCALGADGSIEFHGRIKDMLKVGGENVA
                     ALEIESYLATHPAVKLAQVIGVPDERLLEVPAAFIELNPNAFATAQELIDYCKGKIAS
                     FKVPRHIRFVDEWPMSSTKVQKFRLLEQFEA"
     gene            complement(3417..>3788)
                     /locus_tag="AC16VR_07820"
     CDS             complement(3417..>3788)
                     /locus_tag="AC16VR_07820"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6011102.1"
                     /translation="INTIGPLLISRAFKSNLAASGDGKLMIVTSQLAASTWPMGGMYI
                     YSTTKAGVSKVGQILALDWKEEPITVGLMHPGWVQTDMGGAHAEITAEESASGIRSVI
                     AGMTKADSGSFYKWNGEIHPW"
BASE COUNT          613 a         1196 c         1233 g          746 t
ORIGIN      
        1 tgtggagtgc gcgatgacat cgctgtcata atcgcggtgg aacaggatat acacagttat
       61 gacagcgctg tcaaatttgt ctccggaatt gtgcgggcgc gcatcgggtc cccgcccggg
      121 gccgctcgtg gggacgacgg cggccttccc acttgcgttt gatgttttgc tggagaggtc
      181 cgcaaaaaaa aacgggcgtc cacaggacgc ccggttgatt ggtgatggcg gggccccggt
      241 cggcccgggc cgctaaatcc ggcccatcac gtctccgaac agttcctcgt ccgacggcag
      301 ctccttggtc ttctccaggg gcttcgacac ccaggtctcg ttgatgagat cccgccagga
      361 gatgttgtgg ttggtgccgt tgccgcccca ggacccgcag cccagggaga acgtctgacg
      421 catgccgttc cacaggttgc cgctgttcga cgctgcctga ggctggttga ccatgacccg
      481 tgaggtcttg gtggcgttgg ccagcttcat gatgttgtcg tcgctggtgg agtagatccc
      541 gcaggaatgc ccctggccct ggtacgactg gatgttgttg gtcagctcga tggcgtgatc
      601 gatgtcccgg gctctgtaga gcgccatgat caccgacagc ttctcgccgg agaagggatg
      661 ctccgggccc cagccgtcct ccgggacgat gtagaagctt ctgccctcgg gcagctcgat
      721 ccccgccatg gcggcaatct tctccgccgg ctgggcgacg atggccgtgt tgaggtgccc
      781 ctcgtcgtcc cacagcgtgc tctgcagctt ggccttttcc tcggcgttgc acagatagcc
      841 gccctggtgc tccagcttgt tcatcatctc gtcgtaaatg gcgtccacca ggatcaccga
      901 gttgtcggcg gagcaggagg cagcgaggtc taaggtcttg gacaggcgaa tcttctctgc
      961 ggcgtcttcc agatcggccg tctcgtcgac cgtgatcacc gcgttgccca ccccgacacc
     1021 cagggcgggc gtgccgctcg agtaggccgc gtggaccatg gcgccgccgc ccgtggctag
     1081 cacccggtcg cactgccgca tcagctcgtt ggtaagctcc aggctgggtg attcgatggc
     1141 gatgaccaga tccgccggcg cgccgcaggc ggcgatggcc tcgcgcatct tgtcgcagat
     1201 catcttgttg gtgagctttg cccgcggatg aggcgcgacg atgatggagt tgcgcccctt
     1261 cacggcgtga atggccttga tcaccggcgt ggcttccggg ttggtggatg gcgccagggc
     1321 accgatcacg cccatgggct tggcgatctt gacgatgttg cgctcggcgt cttcttccag
     1381 gatacccacc gacttgtcgt tgatgatgtc catcagcgtc gcgcgggtct ttcggtggat
     1441 cttcaggtac ttgccgtcgt agttaccgag ctgggtctcg tccacggtga actgggcgat
     1501 ctcctcggcc acgcccggct tcgctacgca ccagaccatg gcccggatga gatcgtccac
     1561 ctgctcctgg gtgtagtttt cgatctgcgc ctgcgctgcg cgcgagcgct cgatgagccc
     1621 gcgaatctct tcttgcgggg tcaactcttg atgggcggcc tgctcagcca tagcggaatc
     1681 tcccttgaca acgatggtgg cccggatgcg ggaccgccga accggatact gtacacaacc
     1741 gtatctggcc tgacaaaagc ctttctcaag cttcgaactg ctccaggagc cggaacttct
     1801 gtaccttggt ggacgacatg ggccactcgt ccacgaagcg gatgtggcgc ggaaccttga
     1861 agctcgcgat cttcccttta cagtagtcga tcagctcctg cgcagtggcg aaggcattgg
     1921 ggttcagctc gatgaaagcg gcgggcactt ccagcagccg ctcgtcgggc acgccgatca
     1981 cctgggcgag cttcactgcc ggatgcgttg ccaggtaaga ctcgatctcc agcgccgcca
     2041 cgttctctcc ccccaccttg agcatgtcct tgatgcgtcc gtgaaactcg atggagccgt
     2101 cggcgcccag cgcgcacaga tcgccggtcc ggaaccagcc gtcggcaaaa gcctccgcgt
     2161 tcttctccgg cgccttgtag tagccgtcga aaacggcgta gccgcgtacg aggatctcgc
     2221 cgcgttctcc cggtcccagc tcggcgccgg tatccggatc caccactttg acctcgacgc
     2281 cgggaaatgg ccggccgcag gtggtgagac gctgctccag gctctcgtcc gggtggttga
     2341 aggcgatcac gccgcccacc tcggtgagcc cgtaggcgcc cgtctgcacg gcttgaggaa
     2401 acgcgtcctg aaatttccgc agcacgtccg gcggcgcgac gttgttgatc cggcggatac
     2461 ggctcaagtc ccgggtggtg aactcgggat ggttgatcag ctcgttggtg accgtcggaa
     2521 aggacgggaa ggcaacggtg gttgcgtcgc gctccatcat cgccagggcc ccgccggcct
     2581 cgacgtgggt catggactgc agggcggcac cggcatccat gcagcagatc atgggcagga
     2641 tggaggccat gtggaacatc ggcaggggag cccagaagca atcggtctcg tcgaggaagt
     2701 agcgctcgcg gttcatgttg aagccgtttc gcaccagcac ctcgtggctg agcgggcaac
     2761 ccttcggatc cgacgtggtg ccggaggtgt acatcatgat ggcctggtcc cgcacccggg
     2821 cacagacccg gcgggcctcc acgtcctcga cttggtaccc ggacgccaac gcgtcgagct
     2881 cggcccgggg caagatgccg gcaggcgccg agcttccgag catcacgacc gtcttgagct
     2941 tcggcgcgcc gggcagctcg agagcccggg gatccgcatt ggcggacagc tcggggaacg
     3001 cttcctgcag cagctcggcg aagtcggcgt actcggagat gacgtcgctg atcaggatgg
     3061 ccgagagatc ggcgttctcc accacgtaag ccagctcccg ggccttgtag cgggcgttga
     3121 tcggtaccgc catggcgcct agcagctgga cggcgaaaag gcactccacg tagtccaggc
     3181 agttgggcat caggatgccg acgtggtcgc cccggtcgac gcctaaagcc aacaggctcc
     3241 ttgcgcgggc gtaggcgccc tctcgcagct ggtcgtaggt gagccgtgcc tcgggaaaga
     3301 tgatggcgtc acggtcgccg tggcgatccg ccgcgcgcag cagcagatcg ccaagggtgg
     3361 tgacctcgat gcggtgtgag ctcaagccga caccttcacg ggtgccagga aacctgctac
     3421 cagggatgga tctcgccgtt ccacttgtag aagctgcccg agtctgcctt ggtcatgccg
     3481 gcgatcacgc tgcggatgcc cgatgcgctc tcctcggcgg tgatctcggc gtgggcaccg
     3541 cccatgtcgg tctgcaccca gcctggatgc atgagaccca cggtgatggg ttcttccttc
     3601 cagtccagcg ccagaatctg accaaccttg ctcaccccgg ccttggtcgt ggagtagatg
     3661 tacatcccgc ccatgggcca cgtggaggcc gcgagctggc tcgtgacgat catcagcttg
     3721 ccgtcgccgg aagctgccag gttgctcttg aacgcccggg agatgagcag cggcccgatg
     3781 gtgttgat
//
LOCUS       DBNHQR010000353         2322 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_540370, whole genome shotgun sequence.
ACCESSION   DBNHQR010000353 DBNHQR010000000
VERSION     DBNHQR010000353.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_540370"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /gene="proB"
                     /locus_tag="AC16VR_07825"
     CDS             <1..1053
                     /gene="proB"
                     /locus_tag="AC16VR_07825"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HIR6011103.1"
                     /translation="TSAMGSWCEQVNRLMDAGVQVLIVSSGAVAEGVSRLGWSSRPVG
                     VHEQQAAAAVGQMGLMQAYERAFAGHARHTAMVLLTHDDLADRERYLNARATLTRLLD
                     LGVIPIVNENDTVATDEIRFGDNDTLAALVTNLLEADLLVILTDTDGLHERDPRKDSQ
                     APLVSRADAADPRLDVMAGSTSGALGRGGMVTKIQAARIAARSGAHTVITSGRIDGVL
                     NRVLEGADVGTLLLASMNPLDARKRWIAGQLKVKGELELDRGAVEALRRRGVSLLPVG
                     VSAVRGRFVRGDVVRCVDGDGLAIAQGLVNYSSEEAARLLRAGSDEIADRLGYAAEPE
                     LVHRDNLVLLSGGSKP"
     gene            complement(1054..1320)
                     /gene="rpsT"
                     /locus_tag="AC16VR_07830"
     CDS             complement(1054..1320)
                     /gene="rpsT"
                     /locus_tag="AC16VR_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011336008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HIR6011104.1"
                     /translation="MANSPQARKRARQAEKRRRQNASQRSMVRTYIKRVDAAIQSREY
                     DAASAALNDAIPVIDRMASKGIMHKNKAARHKSRLNAKVAALKA"
     gene            1380..>2322
                     /gene="murJ"
                     /locus_tag="AC16VR_07835"
     CDS             1380..>2322
                     /gene="murJ"
                     /locus_tag="AC16VR_07835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HIR6011105.1"
                     /translation="MSTGTEKPEQQAAALEGPQNVAAQGGIVSGMTLLSRITGLVRDM
                     ALSYFFGAQDVADAFFVAFRIPNFFRRLFAEGAFSQAFVPVLARYRGGGVGELRRFVA
                     VMQGNLIAALSVFVLLGVIFSPALVAVFAPGFFGDDERFALTSSMVRITFPYLGLISL
                     TAFAGALLNSHHRYAVPAFTPVLLNLSLIAAVLFAADVFATPVFALAWGVLVAGFAQL
                     AFQGPSLRRLGLLVAPRISRTDPGARQVGKLLIPAVLAASVNQINALIDTMLASTLVT
                     GSISWLYYSDRLLELPIGLVAVALGTVLLTTLSRLDSS"
BASE COUNT          323 a          744 c          801 g          454 t
ORIGIN      
        1 acatcggcca tggggtcgtg gtgcgagcag gtgaaccggc tgatggacgc aggagtgcag
       61 gtgctcatcg tgtcctccgg agcggtggct gagggcgtca gccgtctggg ctggagctcg
      121 cggcctgtgg gcgtgcacga gcagcaggcc gcagcggccg tggggcagat gggtctcatg
      181 caggcttacg agcgcgcttt tgccgggcac gcaagacaca ccgccatggt cctgctgacc
      241 cacgacgatc tggcggaccg cgagcgctac ctcaacgccc gggccaccct gacccgcctg
      301 ctcgacctgg gcgtcatccc catcgtcaac gagaacgata ccgtcgccac ggacgagatc
      361 cgcttcgggg acaacgatac tctggcggcg ctggtcacca atctgctgga agccgatctg
      421 ctggtgatcc tcacggacac cgatggcctg cacgagcgcg atccgcgcaa ggattcccag
      481 gccccgctgg tatccagggc cgatgcggcc gatccgcgcc tggacgtgat ggcgggcagc
      541 acttcgggcg cgttggggcg aggcggcatg gtgaccaaga tccaggcggc ccgcattgcc
      601 gcacgctccg gggcccacac cgtgattacc agcggcagaa tagacggcgt gctgaaccgc
      661 gtcctcgaag gggccgatgt gggcacgctg ctgctcgctt cgatgaaccc gctcgatgcg
      721 cgcaaaaggt ggatcgccgg tcagcttaag gtgaagggcg agctggagct cgacaggggc
      781 gcggtcgagg cgctgcgccg ccgcggggtc agccttctgc ccgtaggggt gtctgccgtg
      841 cggggacggt tcgtgcgcgg tgacgtggtc cgctgcgtgg acggcgacgg gctggcgatc
      901 gctcaggggc tggtcaatta cagcagcgag gaggctgccc gattgctgcg ggccgggtct
      961 gacgagattg ccgacaggtt ggggtacgcg gcggagccgg agctcgtgca ccgggacaat
     1021 ctggtgctgc tctccggtgg gtcgaagccc tgatcaggct ttcagggcgg cgactttggc
     1081 gttcaggcgc gacttgtgcc gggctgcctt gttcttgtgc atgatgccct tggaagccat
     1141 ccggtcgatg acgggtatcg catcgttcag ggctgcgctg gccgcgtcgt actcccggct
     1201 ctggatggcc gcgtcgacgc gcttgatgta ggtgcgcacc atggaacgct ggctggcatt
     1261 ttgtcgccgc cgtttctcgg cctggcgtgc gcgcttgcgt gcttgtggac tgttggccaa
     1321 gcgtggctcc tgggggtccg gattgcgaag gcgcggtaat atgccgccga ggtatggtga
     1381 tgtcaaccgg cactgagaaa cccgagcagc aggccgcggc cttggaaggt ccccagaacg
     1441 tggccgcaca gggaggcatc gtttcgggca tgaccctgct gtcccgaatc accgggctgg
     1501 ttcgcgacat ggcattgtcc tactttttcg gggcccagga cgtcgcggat gcgttcttcg
     1561 ttgccttccg catccccaac tttttccgtc ggctcttcgc cgaaggcgcg ttcagccagg
     1621 cgttcgtgcc ggttctggcc aggtacagag ggggtggcgt aggcgagctg cgacgcttcg
     1681 tcgcggtgat gcaggggaac ctcatagccg ccttgtcagt ctttgtgctg ctgggcgtga
     1741 ttttctcgcc cgcgctggtc gccgtgttcg cgcccgggtt cttcggcgac gacgaacgct
     1801 tcgcgctcac cagcagcatg gttcgcatca cgttccccta tctcggcctc atctcgctca
     1861 ccgcattcgc cggggcgctc ctcaacagcc accaccgcta cgccgtgccg gcgttcacgc
     1921 ccgtcctgct caacctgagc ctcattgccg cggtgctctt tgctgccgac gtcttcgcga
     1981 cccccgtgtt cgccctggca tggggtgttc tggtcgccgg gttcgctcag ctcgcctttc
     2041 agggcccctc gctcagaagg ctcggactgc tcgtggcgcc ccggatcagc cggaccgacc
     2101 ccggggcccg gcaggtcggc aagctgctca ttccggcagt gctcgcggcg tcggtcaatc
     2161 agatcaacgc gctcatcgat accatgctcg cctcgacgct tgttacgggg agcatctcgt
     2221 ggctgtacta ctccgaccgg ctgctcgagc tccctatcgg gctggtggcc gttgctctgg
     2281 gcacggtgct cctgacgacg ctgtcgcgcc tggattcctc ag
//
LOCUS       DBNHQR010000354        18359 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_350366, whole genome shotgun sequence.
ACCESSION   DBNHQR010000354 DBNHQR010000000
VERSION     DBNHQR010000354.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 18359)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18359)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18359
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_350366"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..932
                     /locus_tag="AC16VR_07840"
     CDS             195..932
                     /locus_tag="AC16VR_07840"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6011106.1"
                     /translation="MSELVLREDREGLAILTLNRPEALNALSPSLFVELRRHVDDIAQ
                     DVDNVGCVLLRGEGRSFSAGNDLKAIQAGERAPGPHFQAETLDAIEGLPQPVIAAVQG
                     HCYTGSLELALACDLIITARGAKIADTHGKWAMSPTWGMSQRLPRRVGLARAKELMYS
                     GRVIDGEEAAAIGLAVRCVPDEELLEAAVAMARTFLENSWFTLRADKMLVNGGLNHTL
                     ADGLAFERSNSPGRGPDLEERLERFGK"
     gene            complement(952..1542)
                     /locus_tag="AC16VR_07845"
     CDS             complement(952..1542)
                     /locus_tag="AC16VR_07845"
                     /inference="COORDINATES: protein motif:HMM:NF009205.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HIR6011107.1"
                     /translation="MTRLDGVEEAAEVAKQFQFLENTLLKNRTLTLFGEINKDVAQRT
                     AEKLLALAYESDDPIVLYIGSPGGHVESGDTIFDMIRYIKPNVKAVGTGWVGSIATHI
                     YLAPDREDRFCLPNTRFLIHQPAGGFGGSASDVEIHAREIVRTRQRINAIIAEQTGQP
                     LERVEEDTDRDYWMSAEESVEYGIVGRIISSIDELG"
     gene            1603..2541
                     /locus_tag="AC16VR_07850"
     CDS             1603..2541
                     /locus_tag="AC16VR_07850"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019474.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6011108.1"
                     /translation="MPLAPEYQSFLEQLAQVPGPPLHELSPADARAAYRLLRPRDEQI
                     TVAAVNELAAPGPAGSIALRVYRPQGDGPFPMLVNFHGGGWVFGDLDTADTVCRNLCR
                     NTGCLVVSVDYRLAPEHPYPAAIDDAYAATCWLAEHASELEGNGRLAVAGESAGGNLA
                     AAVSLRAREEGGPAIDYQLLFYPVIDADFNRASYEENRSGYLLETATMIWFWDHYVPD
                     RSRRSQAYASPIRADDLGRLPPALILTAEFDPLRDEGNAFAEALKAAGTRAELICCAG
                     LIHDFCGTAHLFESSRKPFEDACAALRRAMSGTPES"
     gene            2586..2873
                     /locus_tag="AC16VR_07855"
     CDS             2586..2873
                     /locus_tag="AC16VR_07855"
                     /inference="COORDINATES: protein motif:HMM:NF015735.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HIR6011109.1"
                     /translation="MLYVMICEDKPDSEALRLANRADHLEWVGGKTAMIRLAGPMLSD
                     DGEHMRGSLFIIQAESADAVRQFNGEDPYTRAGLWGKVTIRPFRQVLPADS"
     gene            2887..3603
                     /locus_tag="AC16VR_07860"
     CDS             2887..3603
                     /locus_tag="AC16VR_07860"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6011110.1"
                     /translation="MRKAVILGVGPDLGLGAQLCKRFAGEGLHVMVAGRTDTSVEAVA
                     GDIRANGGAATAVVADATSEDDVRALFDQAGDDLDLAIYNAGNNMPGRIIDMEADYFE
                     NSWRVGCFGGFLFGREAVRRMVPKGAGTLLFTGASASLRGRAKFGAFNSAKGALRNLA
                     QAMAKEYGADGIHVGHVIIDGPIAGDKIIKAFPEYAQRLGSEGMIGIEGIVEGFAFLY
                     RQPRNAWTFEVDVRTSLEKW"
     gene            complement(3674..4081)
                     /locus_tag="AC16VR_07865"
     CDS             complement(3674..4081)
                     /locus_tag="AC16VR_07865"
                     /inference="COORDINATES: protein motif:HMM:NF024844.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HIR6011111.1"
                     /translation="MALNASDLNAGDTYTECLVENLSRTQIVMYAGTSGDYNPLHSDE
                     VFTTQIAGYPSVFAHGMLTMGMTGRMLTNYVGDGRLTKYGVRFTNQVWPGDTLNATAT
                     VDEVADGIVKLNVETTNQDGAVVLSGYAEARVD"
     gene            complement(4093..4587)
                     /locus_tag="AC16VR_07870"
     CDS             complement(4093..4587)
                     /locus_tag="AC16VR_07870"
                     /inference="COORDINATES: protein motif:HMM:NF024844.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAS1-like dehydratase domain-containing protein"
                     /protein_id="HIR6011112.1"
                     /translation="MSVDRFPIEASHIMMFARSIADDNQIYYDEEYAKGTEPGTIIAP
                     PTFAQASAQFDPDYFLRPKIGEPWFGSAKEATGVKREGGGSGGGGGGGLHAEQHFEYH
                     RHLKPGDVLSAEVKPGKTWEKASKRAGKLLFSETITEYRDQNGELVITARGVGVRTER
                     PVDR"
     gene            complement(4696..5475)
                     /locus_tag="AC16VR_07875"
     CDS             complement(4696..5475)
                     /locus_tag="AC16VR_07875"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6011113.1"
                     /translation="MSEFENILYEVQGPMGLITINRADKHNAISLATLDELHQAVGLA
                     AADDAVRVLTITGAGDKAFASGSDLSEVLHRDFRKALEPIVQGLADQLERMPKPTIAA
                     INGICMGGGLEVALGCDLRIAVPKARFATPEGKLGIIPGGGATARLPRIVGRGWGMEM
                     LLMGEPVDAERALQIGLVTRVVEPDELIPEARRMADHLAGFAPFVPRTMKAMVHFGME
                     ASLAGALMFEKYAQGALVQTDDKVEGITAFLEKRDPEFKGR"
     gene            complement(5517..6173)
                     /locus_tag="AC16VR_07880"
     CDS             complement(5517..6173)
                     /locus_tag="AC16VR_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2959 domain-containing protein"
                     /protein_id="HIR6011114.1"
                     /translation="MANWKTALVAGLVALGSAGCETAYYGAMEQVGVYKRDILVDRVE
                     DAVEAQQDAKEEFADALEQFASVVAVEPSRLKDTYEELSDAYDSAEDRAERVRDRIDS
                     VEDVSEDLFDEWSEEIELIGNPKLKSASASQLRASQRKYDELIKGMRRAERKMEPVLD
                     TFRDHVLFLKHNLNARAIASLKSELTSIEADVAGLIREMEASIAQSQAFIEEMQLVDG
                     "
     gene            6415..7224
                     /locus_tag="AC16VR_07885"
     CDS             6415..7224
                     /locus_tag="AC16VR_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003205055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR6011115.1"
                     /translation="MTAYTDTAIELLMTYGPKFLLALVVLIIGLWIIKRVVKVTSKAM
                     ERGNAEPTLAKFVASLVSVGLKALLIISVASMIGIETTSFIAILGAAGLAVGLALQGT
                     LANFAGGALILLFRPYKVGDWIDAQGVAGTVHAIQIFNTILKTPDNRTIIIPNGAISN
                     GIITNNSTEPTRRVDFVFGIGYGDDIAKAKGIIERLIGADERTLSDPAAQIVVSALAD
                     SSVNLTVRVWVNAADYWGVFFDLTEKVKLAFDEEGVSIPFPQQDVHMHQAG"
     gene            complement(7467..8753)
                     /locus_tag="AC16VR_07890"
     CDS             complement(7467..8753)
                     /locus_tag="AC16VR_07890"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6011116.1"
                     /translation="MAVPEGRRGLWGPASLLARTNLILAVGSLVIALIAIAALNVFVI
                     EPIAERSADDEAALLVLSAQTWVELPPAARPYFEIELAQNHDLIVSAEVRDLPFADLD
                     QPYLSLVEEKLSQRLNTPARLREGDDLIWVTVPMGGYDIQIGFASNRRDIQPLYVGII
                     IVGLGAAVVFITSLLIVLRIARPLVETAERAETYRGGENFEPLPERGPRELVSLTRNF
                     NTMAREITALLANRTTLLAGISHDLRTPLTRMRLALELLPDNVDPKLVERFERNLESM
                     DELITDALRFARGTSEVAQEVELKPFVEDVVASFDQSIPLTSDGDDVRVSVAPGALQR
                     VLINLITNALQHGKGVRVHIAGREVHVLDSGPGIPEEYRERVFQPFFRLDDSRSVATG
                     GSGLGLAIVQQLCQAQGWRVAIQEADNGEIDVFVTL"
     gene            complement(8740..9450)
                     /locus_tag="AC16VR_07895"
     CDS             complement(8740..9450)
                     /locus_tag="AC16VR_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010225330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6011117.1"
                     /translation="MDAEGAKLLVVDDDPEIRELAQAYLSQQGFEVDCVDSGEAMDAY
                     LESNEVDLIILDLMLPGEHGLSIARRLKTDGDVPIIIVSAQGEEIDRIVGLEVGADDY
                     IGKPFNPRELLARVRAVLRRAQRTGQASSAENRVCFGSFELDLSAHQLTADGKTVPLT
                     SGEFDLLAILVSHPNKVLDRDRILDLLTGAERSPFDRSIDVRVTRLRAKIEPDPSTPV
                     FIKTIWGKGYMFCPDGRA"
     gene            complement(9460..9882)
                     /locus_tag="AC16VR_07900"
     CDS             complement(9460..9882)
                     /locus_tag="AC16VR_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011118.1"
                     /translation="MLFTSLSGGAFAADEAVRAGDAASIPNVYAAKSDQELTALAAQW
                     ESLDTAERRALLTEVKMRMARQRGPDGTIRITFQRRYGRIIRNPDGSVVRIETNVVRV
                     RPGAAGNQTYGVGFEQRTADGQREEQSPPVITVKDPSP"
     gene            complement(10146..11330)
                     /locus_tag="AC16VR_07905"
     CDS             complement(10146..11330)
                     /locus_tag="AC16VR_07905"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003292904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR6011119.1"
                     /translation="MKDVVIVDSVRTGLAKSFRGSFNLTRSDDMLAHCIDALLDRNGG
                     VSPHEVEDVVVGAASHVGEQDGNLARLAVVLSKLPITTAGLTINRFCSSGLQSIAIAA
                     NQIATGQTQVAIAGGVDSISMRTRQEAAKAELNKRLVEEKPDIYMAMGNTAEVVARRY
                     QVTREAQDEYALQSQQRYAAAAEAGYVAEEIVPMAATMLKVDKETGEESRVDVVVDRD
                     ECNRPTTTLEGLASLPPAFEEDGSVTAGNASQLSDGASMTLLMSAERAAQLGLEPLGI
                     YRGFTVAGCEPDEMGIGPVFAIPRLLENAGLKQDDVDLWELNEAFASQCLYCRDALGI
                     DNEIYNVNGGSISIGHPFGMTGSRMTGLVLRELKRRGKRYGVVTMCIGGGQGAAGLFE
                     AA"
     gene            complement(11619..11963)
                     /locus_tag="AC16VR_07910"
     CDS             complement(11619..11963)
                     /locus_tag="AC16VR_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011120.1"
                     /translation="MSTIKNWMMALLGVVVLVGFVGCGQDAGSGPADAPAAAEEAAAD
                     ETAGGFIQPAAEAAEEAMDEGMEALEEAADETATEAAAEAAEMMEEAAAEAEEAAESA
                     MGEMNITPPGSN"
     gene            12142..12984
                     /locus_tag="AC16VR_07915"
     CDS             12142..12984
                     /locus_tag="AC16VR_07915"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008940347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR6011121.1"
                     /translation="METITLVDGLCFGEGPRWRDGRLWLSDMHAQQVIAVDGDGHVQV
                     VVEVPNLPSGLGWLPDGDLLIVSMRDRRVLRHDGARLHTHADLSQLASFDCNDMVVDV
                     HGRAYVGNFGFDLHGGEAPRAAELICVEPDGTARIVADDLLFPNGTVITPDGNTLIVA
                     ESWGGRLTAFDIDQAGNLSNRRDWAKLPDGAVPDGICLDSDGGIWTASPATNECLRQI
                     EGGTVTHRVPVNQGAFACMLGGDDGRSLFILTAGSSAPDACRAARSGRVELVTAPFPH
                     AGWP"
     gene            13023..14177
                     /locus_tag="AC16VR_07920"
     CDS             13023..14177
                     /locus_tag="AC16VR_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008060912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6011122.1"
                     /translation="MYENYESPWLNDELRLLQDAAAKFFQQEFVPLNEKWINQGKVDR
                     EAWYKAGEAGLLCAEMPVEYGGGGGDYRHETVIMEEQLATGVTGFGNQVHSSIVAPYI
                     LHYGSEDQKQRWLPKMATGELVGAIAMTEPNTGSDLQAVRTSAIRDGDEYVINGSKTY
                     ISNGQHADLVIVVAKTDPAQGAKGISLIVVETGKSPGFERGRNLKKLGQKAADTSELF
                     FADCRVPVTNRLGEEEGRGFVQLMQQLPQERLNIGQAAVVAMERAIGITLDFVRERRA
                     FGQRILDFQNTRFKLSECKTEALIARTFVDQCVLKLVNGELDAVTASMAKYWCTQKQC
                     EIIDECLQLHGGAGYMDEYEISRMYADARVQTIYGGTNEIMKELIGRSLG"
     gene            14185..15465
                     /locus_tag="AC16VR_07925"
     CDS             14185..15465
                     /locus_tag="AC16VR_07925"
                     /inference="COORDINATES: protein motif:HMM:NF018913.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011123.1"
                     /translation="MIQYWRFYWPLALTGLAMVLAVQFQNAALARFPEAVTELAVFAL
                     AYSAYALFHASLNFVAQLSNVYARSPQGTSRSWRFATAASACIMLPLLALAHTDYGAD
                     ALGSIYGIDDGLVERVTEYLAYLAPLLLISAQRFYYNGLLVQARLTGWVTTLTVVYLT
                     SVLVGLIIGFTLGLKPVHVLVGAEGIALLIQLALSVLIKASRYAPPADPEHADLSYGE
                     LLRFYLPVSTTAVMFALSRPILYAFVARTPNGVLAIAAMRVAFDFATIFQQAANQFRH
                     FFVTFGWNDLGIKRRFLTVICAGITAIMLAVALTPLSELIWGKLMGIPEPVRDIAEHV
                     LVVMCLMPALIVFRNYYHGRLMVERRTSGMAAGGILRVIAILLAAQACTMMGWLNYLS
                     ASFILILGFLVEALVALVAAQKGQGLRANATANM"
     gene            complement(15420..15779)
                     /locus_tag="AC16VR_07930"
     CDS             complement(15420..15779)
                     /locus_tag="AC16VR_07930"
                     /inference="COORDINATES: protein motif:HMM:NF016161.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HIR6011124.1"
                     /translation="MLKSIHLILAYLTVLGFVVRGIWSLVDSPLKNQKWVRISPHVID
                     TALLVLGVALAVQLSVSPLEGWLAAKLAGLLAYIGFGVLTMRASSTPLKLAGFAGALA
                     SVGYIFAVAFARSPWPF"
     gene            15867..17330
                     /locus_tag="AC16VR_07935"
     CDS             15867..17330
                     /locus_tag="AC16VR_07935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HIR6011125.1"
                     /translation="MNIDQAIDQAMRPLADAVSGFIFFSVPVLGTQIPLIVVWLILGG
                     LFFTVYLRFVNIRGFAHAIGLVSGRYHNDQHPGEISQFQALTTAVSGTVGIGNIAGVA
                     VAISMGGPGATLWLIIAGLLGMSTKFAECTLGLKFRRHHEDGSVSGGPMYYLERGLAE
                     RNWPRVGRGLGLFYAASMVIGCLGIGNMFQSNQAAAIFIDVSGGDASYFQDKAWLLGL
                     IMAGAVGLVIIGGIRSIARTTSRLVPGMALLYVISALAIIAINAAELPAAFAAIWAGA
                     FSPEGIAGGLIGVMIIGFRRAVFSNEAGLGSAAIAHSTAKTERPVSEGYVALLEPFID
                     TVVICTMTALVIITTVYEPGLTSGNVSGIELTTRAFASTLSWSPIPLSIAAILFAFST
                     MIAWSYYGLKAFTYLVGHHRVADLGFKIFFLVFVVLGSSVQLGALVDLSDALVFVVAI
                     PNLLGLYLLAPTVRAELRAYESNMPVPNGAAPAPDST"
     gene            17385..18254
                     /locus_tag="AC16VR_07940"
     CDS             17385..18254
                     /locus_tag="AC16VR_07940"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6011126.1"
                     /translation="MEGPTSHVYFSQRLRLHYVDWGNPDAPPMLMIHGGRDHCRNWDW
                     VARSLRDRYHIIAPDLRGHGDSQWMLGGSYNQIDYVYDIAQLLTQKDMTPVTIIGHSL
                     GGSIALTYSGLFPETVKKLVAIEGMGPPPEFIKERLAVPVADRLHQWISDLRELSGRI
                     PRRYATLEEAYERMQTENPHLSEEQARHLTIHGSNQNEDGTYSWKFDNYVRAFPPIGI
                     DFEEQYKLHGRITCPTLLIRGTESWASDPSEDGRAARFQNATVRNIERAGHWVHHDQL
                     DEFLEIVDGFLAD"
BASE COUNT         3258 a         6054 c         5652 g         3395 t
ORIGIN      
        1 cacaggcgcg ggaagtcggc agcggggtga cgccggtagg acccgccgcc ggggtagccg
       61 cattcgcccg ctacttcaac ctgcgcgcca gctccatcgc tggaggcacc aacgaggtgc
      121 agaagaacat cgtcgccaag ctggtcctcg gcctgtgaac ggggcgctgg ccgacaactc
      181 tcaagggaag tgacatgagc gaactcgtac tgcgggagga ccgggaaggt ctggcaatcc
      241 tcaccctgaa cagaccggaa gccttgaacg cgctgagccc cagcctgttt gtggagctgc
      301 gccgccacgt ggacgacata gcgcaagatg tcgacaacgt gggctgcgtg ctgctgcgcg
      361 gcgaaggaag gtccttctcc gccgggaacg acctgaaagc cattcaggcc ggggagcgcg
      421 cgccgggccc gcacttccag gccgaaacgc tggatgccat cgaggggctg ccacagccgg
      481 tcatcgcggc cgttcagggc cattgctaca cgggctcgct ggagcttgcg ctggcctgcg
      541 atctcatcat caccgcccgc ggcgcgaaga ttgcggacac ccacggcaag tgggcaatgt
      601 caccaacctg ggggatgagc cagcgcctgc ctagaagagt cggcctggct cgcgccaagg
      661 agctcatgta cagcggccgc gtcatcgacg gtgaggaggc ggccgccatc ggtctagcgg
      721 tgcgctgcgt gccggatgag gaactgctcg aggcagccgt cgccatggcc cgcacgttcc
      781 tggagaactc ctggttcacg ctgcgagccg acaagatgct ggtgaacggc gggttgaacc
      841 acacgctagc cgacggcctt gcgttcgaac gaagcaacag cccaggcagg gggcccgatc
      901 tcgaggagcg gctcgagcgc ttcggcaagt gagaccggtg gtggcccgtc gtcagccgag
      961 ctcgtcgatg ctcgagatga ttcgacccac gatgccgtac tccaccgact cttcggcgct
     1021 catccagtag tcgcggtcgg tgtcctcttc cacccgttcg aggggctggc ccgtctgctc
     1081 cgcgatgatg gcgttgatgc gctgacgggt tctgacgatc tcgcgggcgt ggatctccac
     1141 gtccgatgcg ctcccgccaa agccgcccgc aggctgatgg atgagaaacc gggtgttggg
     1201 gaggcagaag cggtcctcgc ggtcgggcgc gaggtagatg tgcgttgcga tgctcccgac
     1261 ccagccggtg cccacggctt tcacgttcgg cttgatatac cggatcatgt cgaaaatcgt
     1321 atcgcccgac tccacgtgcc caccggggga tccgatgtag agcacgatgg ggtcgtccga
     1381 ctcgtatgcc agcgccagaa gtttctccgc cgtgcgctgg gcgacgtcct tgttgatctc
     1441 cccgaacagg gtcaaggttc ggttcttgag cagcgtgttc tccagaaact gaaactgctt
     1501 ggctacctcc gccgcttctt ccacgccatc aagcctcgtc atccggtgtc tccagatatc
     1561 tttcggttta aacttggggc gcattctaca cgggggatac gaatgccgtt agcgccggaa
     1621 taccagagtt ttctcgagca gctcgcccag gtgccgggac ctcccctgca cgaactgtcg
     1681 cccgccgatg cccgcgccgc ttaccgcctc ttgcggccga gggacgagca gatcaccgtc
     1741 gccgcggtga acgagctggc cgcgcccggg ccagccggat ccattgcgct gcgcgtctac
     1801 cgtccccagg gagatggccc gtttcccatg ctggtgaact ttcacggcgg gggctgggta
     1861 ttcggcgacc tcgataccgc cgataccgtc tgccgcaatc tctgccgaaa cacggggtgc
     1921 ctggtcgtct ccgtggatta ccggctggcg cccgagcatc cctaccccgc ggccatcgac
     1981 gacgcgtacg ccgccacttg ctggctggcg gaacacgcga gcgagctgga gggcaacggc
     2041 cgcctggcag tggccggcga aagcgcgggc ggaaatctgg ccgccgccgt gagcctgcgc
     2101 gcacgggagg aaggcggtcc ggccatcgac tatcagctgc tcttctatcc cgttatcgat
     2161 gcggacttca accgggcctc ctacgaggag aaccggagcg gctacctgct cgagacggca
     2221 acgatgatct ggttctggga ccactacgtg cccgataggt cacgccgttc tcaggcgtac
     2281 gcttcgccca ttcgggcaga cgatctgggc cgcctgccgc cagcgctgat cctgaccgcg
     2341 gagttcgacc cgctccggga cgaaggcaac gctttcgccg aggcgttgaa agccgcgggc
     2401 acgcgggcag agctgatctg ctgcgccggc ctgattcacg acttctgcgg caccgcgcat
     2461 cttttcgagt cgagccgaaa gccgttcgag gatgcctgtg ccgccctgcg ccgggccatg
     2521 agcggcacgc ccgaaagctg aagccgaaca cccccgcaaa actgaagcct gaccgaggaa
     2581 gaagcatgct ttacgtgatg atctgcgaag acaaaccgga cagcgaggcg ctgcgtctgg
     2641 cgaaccgggc cgaccatttg gaatgggtgg gcgggaagac ggcgatgata cggctcgccg
     2701 gccccatgct gagcgacgac ggtgagcaca tgcgcggatc cctgttcatc atccaggccg
     2761 agtcagccga tgcggtgaga cagttcaatg gggaggatcc ctacacgcgc gcggggctct
     2821 ggggcaaggt gaccatccgg cccttcagac aggtcctgcc agcagattca tgaggcgacc
     2881 cagaccatga gaaaagccgt catcctcggc gtcgggcccg acctcgggct cggcgcccag
     2941 ctgtgcaaac gatttgccgg cgagggtctc cacgtgatgg tcgccggacg cacggatact
     3001 tccgtggagg cagtggccgg ggacatccgc gccaatggcg gcgccgccac cgccgtagtg
     3061 gcggacgcga cttcagagga cgatgtccgc gcccttttcg accaggccgg cgacgacctc
     3121 gacctggcca tctacaacgc cggcaacaac atgcccggcc gcatcatcga catggaagcc
     3181 gactacttcg agaacagctg gcgcgtgggc tgctttggcg gtttcctttt cggccgggag
     3241 gccgtccgcc gcatggttcc aaaaggggcc gggaccctgc tgttcaccgg tgcgagcgct
     3301 tcgctgcgcg gtcgcgccaa gttcggcgcc ttcaactcgg ccaagggcgc cctacgcaat
     3361 ctggctcagg caatggccaa ggagtacggg gcagacggca tccacgtggg tcacgtgatc
     3421 atcgacggac ccatcgcggg agacaagata atcaaagcct tcccggagta cgcgcagagg
     3481 ctggggtcgg aaggcatgat cggcatcgaa ggtatcgtcg agggcttcgc cttcctgtac
     3541 cgccaaccgc gcaacgcctg gacgttcgag gtggacgtgc gcacttccct ggagaagtgg
     3601 taacccgggc agaccccctg agcccatcgg cgagaccacg aaggtccacc gggcctcatg
     3661 gcctctgccc cgttcagtcg acgcgggctt ctgcgtaacc ggaaagcacc accgcgccat
     3721 cctgattggt ggtttccacg ttcagcttca cgatgccgtc ggccacttcg tccaccgtgg
     3781 cggtcgcgtt cagcgtgtcg cccggccaga cctggttggt aaagcgcacg ccgtacttgg
     3841 tgagccgtcc gtcccccacg tagttggtca gcattcggcc ggtcatgccc atggtcagca
     3901 tcccgtgggc gaaaaccgac gggtagccgg cgatctgcgt cgtgaacacc tcgtcggagt
     3961 gcagcgggtt gtaatccccc gatgtgccgg catacatgac gatctgggtc cggctcaggt
     4021 tctcgacgag acactcggtg taggtgtcgc cggcgttgag gtcgcttgcg ttcagtgcca
     4081 tctctccctc ccttatcggt ccaccgggcg ctcggtgcgc acacctacgc cgcgggcagt
     4141 gatcaccagc tcaccgttct gatcccgata ctcggtgatc gtctcactga acagcagctt
     4201 gcccgcccgt ttgctcgcct tctcccaggt cttgccgggc tttacctccg cgctcaggac
     4261 gtcgccgggc ttcaggtgcc ggtgatactc gaagtgctgc tcggcatgca ggccgccgcc
     4321 tccaccgccc ccgctgccgc cgccttcgcg cttgaccccg gtggcttctt tggcagagcc
     4381 gaaccagggc tcaccgatct tcggccggag gaaataatcc ggatcgaact gggcgctcgc
     4441 ctgggcgaac gtcggtgggg cgatgatggt gcccggctcg gttcctttcg cgtactcctc
     4501 gtcgtagtag atctgattgt cgtcggcaat cgagcgtgca aacatcatga tgtggcttgc
     4561 ctcgatcggg aatctgtcca cggacatcgt cgtttccccc tcgtgttcgt tgtgcgcgtt
     4621 ggcgctctac ccctcgccga tatacggcgt ctcagagcaa aggagcggaa gtatccccca
     4681 atggtctggg ggggatcaac gacccttgaa ttccgggtcg cgcttctcca gaaaggccgt
     4741 gatgccttct actttgtcat cggtctgaac caacgccccc tgggcatact tctcgaacat
     4801 gagcgcgccg gccaggctcg cctccatgcc gaaatgcacc atcgccttca tcgtgcgggg
     4861 cacgaagggg gcgaagccgg ccaggtggtc ggccatgcgg cgtgcctcag ggatcagctc
     4921 gtccggctca acgactcgcg tcacgagacc tatctgcaga gccctttcgg catctaccgg
     4981 ctcgcccatg agcagcatct ccatccccca gcccctcccc acgattcggg gcaggcgggc
     5041 cgtggccccg ccaccgggga tgatgcccag ctttccttcg ggggtcgcaa agcgcgcctt
     5101 cggcaccgcg atcctcaggt cacagccgag agcgacttcc agacctccac ccatgcagat
     5161 cccgttgatg gccgcgatgg tcggcttcgg catgcgctcc agctggtccg ccaggccctg
     5221 cacgatgggc tcgagcgcct tcctgaaatc gcggtgcagg acctcagaga ggtcggatcc
     5281 cgaagcaaag gccttatccc cggcgccggt gatggtcagc accctcaccg cgtcgtccgc
     5341 cgcggcgagg cccaccgcct gatgcagctc gtctagggtg gccagggaga tcgcgttgtg
     5401 cttgtccgcc cggttgatgg tgatgagccc catggggccc tggacttcgt agaggatgtt
     5461 ctcgaattcg ctcacgtgcc tggttccgtt gtttggccca tcgtctgctg cgcgggtcag
     5521 ccgtcgacca gctgcatctc ttcgatgaag gcctgggatt gcgcaatgga cgcttccatc
     5581 tcgcggatga ggcccgccac gtccgcttct atggaggtta gctcgctttt cagggacgcg
     5641 atggcccggg cgttgagatt gtgcttcaaa aacagaacgt ggtcccggaa agtgtccagc
     5701 accggctcca ttttccgttc cgcccgccgc attcccttga tcagctcgtc gtactttcgc
     5761 tgtgaggctc gcagctggct ggcgctggcg cttttgagct tcgggttgcc gatcagctcg
     5821 atctcctcac tccactcgtc gaaaagatct tccgagacgt cttcgaccga atcgatacgg
     5881 tcccgaaccc gctcggctcg atcctccgcc gaatcgtagg catccgacag ttcctcgtag
     5941 gtgtccttta gtctcgatgg ctctacagca accacgctgg cgaactgctc taaagcgtct
     6001 gcgaactcct ccttggcgtc ttgctgggct tccacggcgt cctcgacgcg atccacgagt
     6061 atgtcgcgct tgtaaacgcc aacctgctcc atcgcaccgt aataggccgt ttcgcagcct
     6121 gctgacccca gcgctacgag cccggctaca agcgccgttt tccaattcgc caccagcctt
     6181 ctcccaaccc gatacaaacc ctttccacga cccgaccaat ctcaacgaac tcgggaacac
     6241 ggtgctgaac gctcaggtcg ggctgttgtt tctcgcgccg atccttagga caatcgcgtc
     6301 ccaaatcagg cgctcaacgc agaagcgcgc gcatagcgtg cccgagcccc atcaaaacaa
     6361 caaccactga aacacgggag gccacacccc atggaggaga tttccacaga gcagatgact
     6421 gcttacaccg acaccgccat cgagctgctg atgacctacg gacccaagtt cctgttggcg
     6481 ctcgtcgttc tcatcatcgg cctgtggatc atcaagcgcg tcgtcaaagt cacgtcaaag
     6541 gcgatggagc gtgggaacgc cgagcccacc cttgcgaagt ttgtcgcaag cctcgtctcc
     6601 gtaggcttga aagccctgtt gatcatcagc gtggcatcga tgatcggcat agagaccacg
     6661 tccttcatcg ccattctcgg tgccgccggc ctggccgtcg gtctggctct gcagggcacc
     6721 ctcgcgaatt ttgcgggcgg cgctctgatc ctgctctttc gcccttacaa ggttggtgac
     6781 tggatcgacg ctcagggcgt tgcaggaacg gtgcacgcca ttcagatttt caacacgatt
     6841 ctgaagacac cggacaaccg gaccatcatc atcccgaacg gcgccatatc gaacggcatc
     6901 atcaccaaca actccaccga gccgacccgc cgcgtggact tcgtgttcgg catcggctac
     6961 ggggacgaca ttgccaaagc caaaggcatc atcgagaggc tgatcggggc cgacgagcgg
     7021 accctttccg accccgcggc tcagatcgtg gtttccgcgc tcgccgacag ctcggtcaac
     7081 ctcaccgtaa gggtctgggt caacgccgcc gattactggg gcgtgttctt cgatctcacc
     7141 gagaaggtca agctcgcatt cgacgaggag ggagtttcca ttccgttccc tcagcaggac
     7201 gtgcacatgc accaggcagg ctgacgctcg ctggggtgtt ttcgggcgcc gcctgaccag
     7261 cggcgctcga gcaaaaaaaa acgccggctt ggagagaaag ccggcgtcta aaggaccttt
     7321 tacaaggtct caaggggata gagattgcct catggggaag cggtttaacc cgcttcgagg
     7381 tccaatatga gggaaagacg tttcgccgcg atttcctgcc gcgacctttt tgtgacattt
     7441 tgtttcccga tgcccaccgg ttcgacctag agcgtaacaa agacgtcgat ctcgccgttg
     7501 tccgcttcct gaatggccac tcgccagccc tgcgcctggc aaagctgctg gacgatggcc
     7561 agcccgaggc cgctgccacc cgtggctaca ctgcgtgaat cgtcaaggcg aaagaaaggc
     7621 tgaaagaccc gctcccggta ctcttcgggg atgcccggcc cgctgtcgag gacgtgcacc
     7681 tcccggccgg caatgtggac gcgcacgccc ttgccgtgct gcaaggcgtt ggtgatcagg
     7741 tttatcaaca cacgctgcag cgcgcccggg gcgacgctga cgcgaacgtc gtctccgtca
     7801 ctggtcagcg ggatggactg gtcgaagctg gcaaccacat cctccacgaa gggcttgagc
     7861 tccacctcct gcgccacctc ggaagtcccc cgcgcaaatc gcagggcgtc ggtgatcagc
     7921 tcgtccatgg attcgagatt gcgctcaaat cgctcaacga gcttggggtc gacattgtcc
     7981 ggcagcaatt cgagcgccag ccgcattcgc gtgagggggg tgcgcaggtc gtgggagatc
     8041 ccggcaagca gcgtcgtgcg attggctagc agagcggtga tctcacgcgc catggtgttg
     8101 aaattgcggg tcagcgagac cagctcccga ggcccacgct ccggcagcgg ctcgaaattc
     8161 tcgccaccgc gataggtctc ggcgcgttcg gcggtctcga ccagagggcg cgcgatcctg
     8221 agcacgatga gcagcgacgt gatgaacacc acggcagcac caagcccgac gatgatgatc
     8281 ccgacgtaaa ggggctggat gtcgcgccgg ttggacgcga agcctatctg gatgtcatac
     8341 ccgcccatgg gcacggtgac ccagatgagg tcatcgccct cacgcaggcg agccggcgtg
     8401 ttcaggcgct ggctgagctt ctcctcgacc agagaaaggt agggctggtc caggtctgcg
     8461 aaaggcagat cgcgaacctc tgcgctcacg atcagatcgt gattctgcgc cagctcgatc
     8521 tcgaagtacg ggcgggctgc cggcggcaac tctacccagg tctgggcaga aagcacgagc
     8581 agggccgctt cgtcgtccgc cgagcgctcc gcgatgggct cgatgacgaa aacgttgagc
     8641 gcggcgatgg cgatcagcgc gatgaccagc gaaccgactg ccaggatgag gttggtccgt
     8701 gcgagcagag acgccggacc ccagagcccc cgccgcccct caggcacggc catcggggca
     8761 gaacatgtac cctttacccc agatggtctt gatgaatacc ggcgtggagg gatccggctc
     8821 gattttcgcc cgcagccggg tcacccgcac gtcgatgctg cgatcgaagg gcgaccgctc
     8881 ggctccggtc agcagatcca ggatgcggtc tcggtccaga accttgttgg gatgggagac
     8941 gaggatcgcg agcagatcga actctcccga cgtgagcggt accgtcttgc catctgccgt
     9001 cagctgatga gccgaaaggt ccagctcgaa gctcccgaaa cacacgcggt tctctgcgga
     9061 cgacgcctgc ccggttcgct gagcgcggcg cagcacggct cgaacccggg caagcagctc
     9121 gcgggggttg aacggcttgc cgatgtagtc gtctgcgcca acctcgagcc cgacgatgcg
     9181 gtcgatctct tcgccctggg cagacacgat tatgatgggg acatcgccgt cggtcttgag
     9241 ccggcgggcg atcgaaaggc cgtgctcacc gggcagcatg agatcgagaa tgatgagatc
     9301 cacctcgttg gattccaggt aggcgtccat ggcctcgccg ctgtcgacgc agtccacctc
     9361 gaatccctgc tgggagagat aggcctgtgc cagctcacga atctctgggt cgtcgtcaac
     9421 gactagaagc ttggcacctt cagcatccac ggcgggggct tacggtgagg gatccttgac
     9481 cgtgatgacc ggcggcgact gctcctcccg ctggccgtct gcggtccttt gctcgaagcc
     9541 tacgccgtag gtttgattgc ccgccgcgcc ggggcggact cgaaccacgt tggtctcgat
     9601 ccgaaccacg gatccgtccg gattgcggat gatgcggccg taacggcgct gaaacgtgat
     9661 gcggatggtg ccgtccggac cgcgctggcg cgccatacgc atcttgacct cggtcaacag
     9721 ggcccggcgc tcggcggtgt ccagggattc ccactgggcg gcgagagccg tcagctcctg
     9781 gtcggatttg gctgcataga cgttcgggat gctggccgca tcgcccgcac gcacagcctc
     9841 gtcggcggcg aaagcgccgc cggacagtga cgtgaagagc aaaacgctca gggtcaggct
     9901 tctgatgaac gtaacccctg ttccattcgg agctccgacc gcatccgcgc accggatgct
     9961 cccattttcc gccccgtgat gattggacca ctataaatgc ttatggtgcc ggcagcctct
    10021 acgtgtccta caagaacgcg cacaggcctc gcgaattatc cccagagcga cggcaattct
    10081 gactctgcgc tgtttcagcg ccatttccct tgggtttcaa tttgttactt aaccccgggg
    10141 gctgatcagg ctgcctcgaa cagacctgct gcaccctggc caccgccaat gcacatggtg
    10201 acgactccgt acctcttgcc acgccttttc agctcgcgga gaaccaagcc cgtcatgcgc
    10261 gagccggtca tcccgaacgg atggccgatg ctgatgctgc cgccgttgac gttgtaaatc
    10321 tcgttgtcga tgcccagcgc atcccgacaa tacaggcact gcgacgcgaa cgcctcgttg
    10381 agctcccaca gatcgacgtc atcctgcttg agacccgcgt tttccagcag ccgcgggatg
    10441 gcgaacaccg ggccgatacc catctcgtcg ggctcgcagc cggccacggt gaagccccgg
    10501 tagatgccca acggttctag gcccagctgc gctgcgcgct cagcgctcat gagcagcgtc
    10561 atggacgcgc cgtcggagag ctgagaagca tttcccgcag tgacggagcc atcttcctcg
    10621 aaggccggcg gcagcgatgc cagaccttcc agggtggtgg tggggcggtt gcactcgtcc
    10681 cggtcgacga cgacgtccac gcggctttct tcacccgtct ccttgtccac cttgagcatg
    10741 gtggccgcca tgggcacgat ctcttcggcg acatatccgg cctcagcggc ggccgcgtat
    10801 ctttgctggc tctgcagggc atactcgtcc tgggcttcgc gggtcacctg atagcgccgc
    10861 gcgaccacct cggcggtatt ccccatggcc atgtagatgt ccggtttctc ctccaccagc
    10921 cgcttgttga gctcggcctt ggccgcctcc tgacgggtac gcatggagat cgagtcgacg
    10981 ccgcctgcaa tggcgacctg agtctggccg gtcgcaatct ggttggcggc gatggcgatg
    11041 gactgcaggc cggacgagca gaagcgattg atggtcaggc cggcggtcgt gatcggcagc
    11101 ttcgacagca ccacggccag tcgggccagg ttgccgtcct gctcgccgac gtggctagcc
    11161 gcccccacca cgacgtcttc cacctcgtgc ggcgatacgc cgccgttcct gtcgagcagc
    11221 gcgtcgatgc agtgcgcgag catgtcgtcg gagcgggtga ggttgaagct cccacggaag
    11281 gatttggcga gcccggttcg aacgctatcg acgatgacca cgtctttcat gatgttcccc
    11341 tttcaagttg ttcgctcaac gcaggccctg ggttttgcag cgccccggca aaagtgcccc
    11401 gggagcgtac cagaaaaaaa aagacacggt ctcggctggc tgggcccgca ccctcgtcat
    11461 acgcgcgcca atgacgccga ccgcttcgct gcagggcaaa cagaaacggc ccgaggctgg
    11521 cgcaccgcgc cgcgttcgga ccgttacctg atcacgagcg ggccagactt cgaacagttc
    11581 gaaactgcac tgacccgttg gctggaagtg gaaggcggct agttggagcc cggcggggtg
    11641 atgttcatct cccccatggc cgattcggct gcctcttctg cctccgcggc tgcttcttcc
    11701 atcatctcgg ctgcctctgc tgcagcttcg gtggcggttt cgtcagcggc ttcctcaagc
    11761 gcttccatcc cctcgtccat agcctcttcg gctgcttcag cggcaggctg aatgaaccca
    11821 cccgcggtct catccgcggc tgcctcttcc gccgcggcgg gcgcatcagc gggtccggac
    11881 cctgcgtcct ggccgcagcc gacaaacccg acgagaacga caacgccgag cagcgccatc
    11941 atccagttct tgatcgtact catctatccc tcctcgttgc tttcgaccca gggccagacc
    12001 ctgcaggctg agcagacgca ggccttattg ccaccgtcat tccccagcgg tcgaggtatt
    12061 cgcaagacta cagaaaaagg tctggccaac caacctgtac ctgtcggcgt gctaagctaa
    12121 gcggccacct ctggagggtc catggaaacg atcactttgg tcgatggcct gtgcttcggc
    12181 gaggggccgc gctggcgaga cggccgtctg tggctgtctg atatgcacgc tcaacaggtg
    12241 atcgccgtgg acggcgacgg ccacgttcag gtcgtggtcg aggtgccgaa cctcccctcc
    12301 ggcctgggat ggctcccgga cggcgacctc ctgatcgtct ccatgcgcga tcggcgcgtt
    12361 cttcgccacg acggcgcccg cctgcacacc catgctgacc tgtcacagct ggcaagtttc
    12421 gactgcaacg acatggtggt ggacgtccac ggtagggcct acgtgggaaa cttcggtttc
    12481 gatcttcacg gcggagaagc gcccagggcc gcggagctaa tctgcgtgga acctgacgga
    12541 accgcgagga tcgtcgcaga cgacctgctc tttcccaacg ggacggtcat cacgccggac
    12601 ggcaacacgc tgatcgttgc agaatcctgg ggcggccgac tgaccgcttt cgacatcgac
    12661 caggccggca acctgtcgaa ccgcagagac tgggccaagc tccccgacgg cgccgtgcct
    12721 gacggcatct gcctggattc cgacggggga atctggactg cctcgcccgc gaccaacgag
    12781 tgcctgcgcc agatcgaagg gggcaccgtc acgcaccggg tcccggtgaa ccagggcgct
    12841 tttgcgtgca tgctcggggg agacgacggc cgctccctct tcatcctcac cgccgggtca
    12901 tcggcgcccg acgcctgccg tgcggcccgc tccggacgcg tggagctcgt taccgccccg
    12961 ttcccgcacg ccggctggcc gtaaaagccg cgcaaccgac tcacacgagg aaacaagacg
    13021 tgctgtacga aaactacgag tcaccctggc tgaacgatga gcttcgcctg ctccaggacg
    13081 ccgcagcgaa gttttttcag caggagttcg tgccgctgaa cgagaagtgg atcaaccagg
    13141 gcaaggtgga ccgtgaagcc tggtacaagg ccggcgaggc aggcctgctc tgcgcggaga
    13201 tgcccgtcga gtacgggggc ggtggcggcg actaccgcca cgagaccgtc atcatggagg
    13261 agcagctcgc caccggggtg accggcttcg gcaaccaggt ccacagcagc atcgtcgccc
    13321 cgtacatcct gcattatggc tccgaagacc agaaacagcg ctggctgccg aagatggcca
    13381 ccggcgagct cgtcggcgcc atcgccatga ccgagcccaa tacgggctcc gatcttcagg
    13441 ccgtgcgcac gtcggcgatc cgcgacggtg acgagtacgt catcaacggc tccaagacct
    13501 acatttccaa cggccagcac gccgatctgg tcatcgtcgt ggcaaagacc gatccggccc
    13561 agggcgcgaa gggcatatcg ctgatcgtgg tggagacggg caagagtccg ggtttcgagc
    13621 ggggacgcaa cctcaagaag ctgggacaga aggccgccga cacctcggag ctgttctttg
    13681 ccgactgccg ggttcccgtc acgaaccgcc tcggcgagga ggaaggacga ggcttcgtcc
    13741 agctgatgca gcagctgcct caggaacgcc tgaacatcgg ccaggcagcc gtggtggcca
    13801 tggaacgcgc catcgggatc acgctcgact tcgtcaggga gagacgcgcc ttcggtcagc
    13861 ggatcctgga tttccagaac accagattca agctgtccga gtgcaagacg gaggccctca
    13921 tcgcccgcac cttcgtggac cagtgcgtgc tcaagctcgt caacggcgag ctcgacgccg
    13981 tcacggcatc catggccaag tactggtgca cccagaagca gtgcgaaatc atcgacgagt
    14041 gcctgcagct gcacggcggt gccggctaca tggacgagta cgagatctcg cggatgtacg
    14101 ccgatgcaag agtgcagacg atttacggcg gcaccaacga gatcatgaag gagctcatcg
    14161 gccgcagcct gggatagttc caggatgatc cagtactggc gcttctactg gcctctcgcc
    14221 ctcaccggcc tggccatggt actggccgtg cagttccaga acgcggcgct cgcccgtttc
    14281 ccggaagccg ttaccgagct ggccgtgttc gcgctggcat acagcgcgta cgcccttttc
    14341 cacgccagcc tgaacttcgt cgcgcaactc tcgaacgtct acgctcgcag cccccagggc
    14401 acctcacgca gctggcgatt cgccaccgct gcgagcgcgt gcatcatgct gccgctgctc
    14461 gcgctcgctc acaccgacta cggcgccgac gcgctgggct cgatctacgg catcgacgac
    14521 gggctcgtcg agcgggtcac ggaatacctc gcctacctcg ctcctctcct gctgatcagc
    14581 gcgcagcgct tctactacaa cggtctgctc gtgcaggccc gattgaccgg ctgggtgacc
    14641 acgctgaccg tcgtctacct caccagcgtg ctggtcgggc tgatcatcgg cttcacgctg
    14701 ggactcaagc ccgtgcacgt tctggtcggc gcggagggca tcgccctgct gatccagctg
    14761 gcgctgtccg tgctgatcaa agcaagccgc tacgcccctc cggcagaccc ggagcacgcg
    14821 gacctgtcct acggcgagct gcttcggttt tatctcccgg tgtcgaccac ggcggtcatg
    14881 ttcgcgctgt cacggcccat cctctacgcg ttcgtggcac gcacgcccaa cggagtgctc
    14941 gccatcgccg ccatgcgcgt agcgttcgac ttcgccacca tttttcagca ggccgccaac
    15001 cagtttcgcc acttcttcgt gaccttcggt tggaacgacc tgggaatcaa gcgccgcttc
    15061 ctcaccgtga tctgcgcggg tatcaccgcg atcatgctgg ccgtggcgct cacacccctg
    15121 tccgagctca tctggggcaa gctgatgggc atcccggaac cggtccgcga catagccgag
    15181 catgtgcttg tggtgatgtg cctgatgcca gctctgatcg tgttccggaa ctactatcac
    15241 ggccggctga tggtcgagcg ccggacgtcg ggcatggccg ctggcggcat tctcagagtc
    15301 atcgcgatac ttcttgcggc gcaggcatgc acgatgatgg gctggctgaa ctacctgagc
    15361 gcgtcgttca tcctgattct gggctttctg gtggaagcgc tggtggcgct cgtagcggct
    15421 cagaaaggcc agggactgcg ggcaaatgcc accgcgaata tgtagcctac gcttgccagg
    15481 gcaccagcga acccagcgag cttcagcggc gtcgagctcg cccgcatcgt gaggactccg
    15541 aatccgatgt aggccaggag cccggcgagc tttgctgcca gccagccttc cagtggcgaa
    15601 acgctgagct gcacggccag cgccacaccg agcacgagca gcgccgtatc gatgacgtgc
    15661 ggcgagatac gcacccactt ctggttcttc agcggagagt cgaccaggga ccagattccc
    15721 ctgaccacaa acccgagcac cgtcaggtag gcaagaatca gatggatcga tttcagcatt
    15781 gtctctcccc gggcggtcct cgatcacttc gtttatgatc ggcctcccag cgcattcaag
    15841 caacccgaca cctcggaaaa ctatctatga atattgatca ggccatcgac caggccatgc
    15901 ggccccttgc ggacgcggtg tccggattca tcttcttctc tgtgcccgtt ctcggaaccc
    15961 agatcccgct gatcgtggtc tggcttatcc tcggggggct tttcttcaca gtgtatctgc
    16021 gcttcgtcaa cattcgcggc ttcgcccacg ccatcgggct ggtcagcggg cgctaccaca
    16081 acgaccaaca cccgggtgag atctcgcagt tccaggcttt gacgactgcg gtatcgggca
    16141 cggtgggaat aggcaacatc gccggggtag ccgtggccat ttccatgggc gggcccggcg
    16201 cgacgctgtg gctgatcatc gccggcctgc tgggcatgtc gacgaagttc gccgaatgca
    16261 ccctcgggct caagtttcgg cggcatcacg aggacggctc ggtttccgga ggcccgatgt
    16321 attatctcga gcgcggcctg gcggagcgca actggccgcg ggtcgggcgc gggctcgggc
    16381 tgttctacgc ggcatccatg gtcatcggct gtctcggtat cggcaacatg tttcagtcga
    16441 accaggcggc agccatcttc atagacgtca gcggcggcga tgcgagctac ttccaggaca
    16501 aggcttggct actgggcctg atcatggccg gcgcggtagg gctggtaatc atcggcggca
    16561 tccgttccat tgcgcgcacc acctcgaggc tggtccccgg tatggcgctg ctgtacgtga
    16621 tctcggcgct tgccatcatc gccatcaacg cggccgaact gcctgccgcc ttcgccgcca
    16681 tctgggcggg cgccttctct ccggagggta tagccggagg cctcatcggc gtcatgatca
    16741 tcggtttccg cagagccgtg ttctccaacg aggccgggct cggctccgct gccatcgccc
    16801 actccacggc gaagaccgaa cgcccggtga gcgaaggcta cgtcgctctc ctggagccct
    16861 tcatcgacac cgtcgtgatc tgcaccatga ccgccctggt catcatcacg acggtgtacg
    16921 aacccgggct gaccagcggc aacgtgagcg ggatcgagct caccacccgc gcgtttgcga
    16981 gcacgctgtc atggtcgccg ataccactgt ccatcgccgc catcctcttc gcgttctcca
    17041 ccatgattgc ctggtcctac tacggcctga aggccttcac ctatctggtg ggccaccacc
    17101 gggtcgccga cctgggcttc aagatcttct tcctcgtctt cgtggttctg ggcagcagcg
    17161 ttcagctggg tgccctggtg gacctctccg acgcgctcgt gttcgtggtc gcgatcccca
    17221 acctgctggg gctgtacctg cttgccccca cagtgcgggc cgagctgagg gcctatgaat
    17281 ccaacatgcc ggtgcccaac ggcgcggcgc cggctcccga cagcacctga gccgggcgcg
    17341 ttataatgcg cgagctctca gcacgccgga acgctttatc gacaatggaa ggacccacct
    17401 cccacgttta cttctcccag cggctcaggc tgcattatgt ggattggggc aaccccgacg
    17461 ctccacccat gctgatgatt cacggcggcc gcgatcactg caggaactgg gattgggtgg
    17521 cccggtcact tcgcgaccgc taccacatca tcgcccccga cctgcgcggt cacggcgact
    17581 ctcagtggat gctcggcggg agctacaacc agatcgacta cgtgtacgac atcgcccagc
    17641 tgctgactca gaaggacatg acgccggtga cgatcatcgg gcactcgctg ggtggctcca
    17701 tcgcgctcac ttactcgggt ctgttcccgg agaccgtgaa gaagctggta gccatcgagg
    17761 gcatggggcc gccgcccgag ttcatcaagg aacggctcgc cgtaccagtg gcggaccggc
    17821 tgcaccagtg gatctccgat ctgcgcgagc tttcgggaag gatcccccgt cgctacgcga
    17881 ccctggagga agcctacgag cgcatgcaga cggaaaatcc tcacttgagc gaggagcagg
    17941 cacggcacct gacgatacat ggcagcaacc agaacgagga cggcacctac agctggaagt
    18001 tcgacaacta cgtacgggct ttcccgccca tcggcatcga cttcgaggag cagtacaagc
    18061 tccatggccg catcacctgc cccaccctgc tgattcgcgg caccgagtcc tgggcgtccg
    18121 accccagcga ggacggccgc gccgcgcgct tccagaacgc caccgtgcgc aacatcgaaa
    18181 gggccggaca ctgggtccac cacgatcagc tcgacgagtt tctggaaatc gtggacggtt
    18241 ttctcgccga ctgagaatga gtcagcgcgc ggtcattctg caccgctccc cctggcaggg
    18301 cgtgttccac gtaaccggcg gcggatcggg tctgctggcc gagctgctga ccacgcccg
//
LOCUS       DBNHQR010000355         1688 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_223544, whole genome shotgun sequence.
ACCESSION   DBNHQR010000355 DBNHQR010000000
VERSION     DBNHQR010000355.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_223544"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..1080
                     /gene="dusB"
                     /locus_tag="AC16VR_07945"
     CDS             91..1080
                     /gene="dusB"
                     /locus_tag="AC16VR_07945"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HIR6011127.1"
                     /translation="MTVTAIGPFKLRNDVLLAPMAGITDAPFRALAWRFGAGSVVGEM
                     VGSREALWHTPKSRLRRCRVDGVEPFAVQIAGADPRDVAESAARHVEEGAQVIDINFG
                     CPVKKVCNKAAGSALLKEPDLVVAIVRAAVAAVPVPISVKMRTGWSPDSRNAVELAQR
                     MEDEGVAAIAVHGRTRACRFNGEAEHGTVAAIKSRVSIPVFANGDIDSPASARRILEE
                     TGVDGVMIGRAALGRPWLLGEVADGVPRVASLGEKLAVVAEHVAALHEFYGEPGTRIA
                     RKHVQWYLQHLSQLGPREVRSRLGKAFNRLEQAQAQLDFLAELPASAIGRRAA"
     gene            1119..1178
                     /locus_tag="AC16VR_07950"
     CDS             1119..1178
                     /locus_tag="AC16VR_07950"
                     /inference="COORDINATES: protein motif:HMM:NF014950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011128.1"
                     /translation="MLGMNRGTLRKKLKGYGII"
     gene            1175..>1688
                     /gene="purH"
                     /locus_tag="AC16VR_07955"
     CDS             1175..>1688
                     /gene="purH"
                     /locus_tag="AC16VR_07955"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939578.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HIR6011129.1"
                     /translation="MSNADARSVARALLSVSDKTGLEEFARGLSALGVEILSTGGTYR
                     ALSEAGIRVTEVSAHTGFPEMMDGRVKTLHPKIHGGILGRRDVDGATMTEHDIEPIDL
                     VVVNLYPFEATIERADCTDELAIENIDIGGPAMVRSAAKNHRDVLVVVDPGDYGDLLE
                     TLQRDGATTLE"
BASE COUNT          280 a          540 c          584 g          284 t
ORIGIN      
        1 gagctgaccg tcgcgcaccc cggcgacatg gccgttaact attctctttc gttccggtga
       61 cgttatgatg tcgccccttt tcttcaaggg gtgaccgtga ccgccatcgg gccattcaag
      121 ctgcgcaacg acgtactgct ggcgcccatg gcggggatca ctgacgcgcc ctttcgcgcg
      181 ctggcctggc gattcggggc cggctctgtg gtcggcgaga tggtcggctc ccgtgaagcg
      241 ctctggcaca cgcccaaatc ccgcctgcgc cgctgccggg tggacggtgt ggagcccttc
      301 gcggtccaga tcgcgggcgc cgacccccgg gacgttgccg agagtgccgc gcggcacgtc
      361 gaagaaggtg cccaggtgat cgacatcaac ttcggctgtc cggtcaagaa ggtctgcaac
      421 aaggctgcag gatcggctct gctcaaggag ccggatctgg ttgttgccat cgttcgcgct
      481 gcggttgccg ccgtgccggt tccgatctcg gtgaagatgc gcacgggttg gtcgccggat
      541 tcccgtaacg ccgtggagct cgcgcagcgg atggaggacg agggcgtcgc agccatcgcc
      601 gtgcacggcc gcacgcgcgc ctgccgcttc aacggcgagg cggagcacgg caccgtggcc
      661 gccatcaagt ccagggtgtc catcccggta tttgccaacg gcgacataga ctcccctgcg
      721 tcggcgcgcc ggatactcga ggagaccggc gtcgacggcg tcatgatcgg ccgtgctgcc
      781 ctgggcagac catggttgct cggcgaggta gcggacgggg tgcccagggt ggcttcgctg
      841 ggtgagaagc tcgccgtcgt ggcggagcat gtcgccgccc tgcacgagtt ctacggtgag
      901 cccggcaccc gcattgcgcg aaagcacgtt cagtggtacc tccagcacct gagccagctg
      961 gggccgaggg aggtccgctc ccgtctcgga aaggcgttca atcgactgga gcaagcgcag
     1021 gcccagctgg atttcctggc tgaactgccg gcctctgcca tcggtcggag agcggcgtga
     1081 tggcgcgcat cggcggcaat aaaagtcgcg cggcgaccat gctcggtatg aatcggggca
     1141 cgctgcgcaa gaaactcaag ggttacggaa tcatatgagc aacgctgacg cgcggagtgt
     1201 ggcccgcgcc ctgctgagtg tgtcggacaa gacggggctc gaggagttcg cccgcgggct
     1261 ctcggctctg ggcgtggaga tcctctcaac ggggggtacc taccgggcgc tctcggaagc
     1321 gggcatcagg gtcacggagg tctccgccca caccggtttc ccggaaatga tggatggccg
     1381 cgtcaagacg ctgcacccga agatacacgg cggcatcctc ggccggcgcg acgtggatgg
     1441 cgcgaccatg accgagcacg acatcgagcc catcgatctc gtggtcgtca acctctaccc
     1501 gttcgaggcc accatcgagc gcgccgactg caccgacgag ctggccatcg agaacatcga
     1561 catcggcggg cccgcgatgg tccgctcggc agcgaagaac catcgcgacg ttctcgtggt
     1621 ggtagacccc ggggattacg gcgatctgct cgagaccctg cagcgcgacg gcgcgaccac
     1681 gttggaga
//
LOCUS       DBNHQR010000356         8254 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_350465, whole genome shotgun sequence.
ACCESSION   DBNHQR010000356 DBNHQR010000000
VERSION     DBNHQR010000356.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8254)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8254)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8254
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_350465"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..868)
                     /gene="gspN"
                     /locus_tag="AC16VR_07960"
     CDS             complement(149..868)
                     /gene="gspN"
                     /locus_tag="AC16VR_07960"
                     /inference="COORDINATES: protein motif:HMM:NF013376.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HIR6011130.1"
                     /translation="MRTLILLGFLLFLTFCIVFAPAGLTGLVLDRVNGVDLTRVNGTL
                     WQGGGDLYVRSEPVGRLQWDVHPATVVAGALGCDFTLSHAHHQLRGTAVVGLNRTFTL
                     DVSGKVGAQTVNGWLEPYDMTISGTMDLSNVLLTFDDVALRDANGVVEWEGGSISYPA
                     GNGIQSSILPPLRATLGPGPAATITTLDDPAAALVTAELLDNGIAKVGVTARMTRLLN
                     LSWTETEPDSAVVLEVEEQVF"
     gene            complement(871..2016)
                     /gene="dapE"
                     /locus_tag="AC16VR_07965"
     CDS             complement(871..2016)
                     /gene="dapE"
                     /locus_tag="AC16VR_07965"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003293424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HIR6011131.1"
                     /translation="MSEGSQNPVLELTVELMRRASVTPSDEGCQELIGQRLAAMGFVN
                     RPMNFGDVSNLWATHGEGGPLFVFAGHTDVVPAGPQDAWTNPPFEPTLHDGFLWGRGA
                     ADMKASLAAMVVATERFLAAHPRHRGTLAYLLTSDEEGPAVDGTRRVVETLQAERAQE
                     MDFCVVGEPSSAHLLGDTVRVGRRGSVNARLTVFGTQGHVAYPELAENPVHTLLPALA
                     ELTNKRWDEGNDGFPPTGLQVSNIHAGTGAPNVIPGSLVVDFNIRFNTEQTSSGLHRQ
                     AAEILDRHGLQYDLEWVVSGEPFLTAPGDLRAAVNKAVGDVTGRTPEASTSGGTSDGR
                     FIAPTGCEVVELGPVNATIHKIDERVATADLLPLADIYQRVLDQLLG"
     gene            complement(2024..3037)
                     /locus_tag="AC16VR_07970"
     CDS             complement(2024..3037)
                     /locus_tag="AC16VR_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012722589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HIR6011132.1"
                     /translation="MSGIFAFAVGTVRRNTAGAPLDFYFPAPVINPEPDLAAQIEGSF
                     SSGAHEVGPAELARFAKAVGDSAMARLLSSLAETDRAMTAVRLDADEPIDSSATAYLK
                     LHLLSHRMALPNTLNLDGIFAHLPNVAWTDRGPIDPEELADAQLQARLAGGHIEVLAV
                     DKFPRMTNYIVPSGVRIADASRIRLGAYLGDGTTVMHEGQINFNAGALGPNMVEGRIS
                     QGVILGEHSDLGGSASTMGTLSGGGEITISIGKHCLIGANAGTGIPLGDRCTIEAGLY
                     ITAGTPVTLIDETGEDVRTMKARELAGGSDLLFIRNGRSGRIECRSNRKAIELNEQLH
                     AHN"
     gene            complement(3110..4306)
                     /gene="dapC"
                     /locus_tag="AC16VR_07975"
     CDS             complement(3110..4306)
                     /gene="dapC"
                     /locus_tag="AC16VR_07975"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008568138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 -
                     succinyldiaminopimelate:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HIR6011133.1"
                     /translation="MNANLEKLQAYPFERLAELLAGVRGNAEFPHIALSIGEPKHAPP
                     EFVINLLSDPDRLRAGLGAYPATRGIPELREAISGWLARRYGVRLDPDNQVLPVAGTR
                     EALFSFGLAALSGKADARVLMPNPFYQIYEGAALLGNGVPHYVNCDRANDYQPDFAGV
                     PDSVWSACEVVYLCSPGNPTGETIPEDTVLWLLEQADRHDFVVAADECYSEIYLDDSH
                     PPAGLLEIAHRAGRDDYARCVAFHSLSKRSSLPGLRSGFVAGDAAILAGYFKYRTYEG
                     CALPLHVQQASAAAWSDEAHVEANRALYRRKFETVGRVLDPHLDLRTPAGGFYHWLHI
                     GEDDTAFARQLFEQCNLTVLPGSFLGRAGGDSNPGGGHVRVAWVASEAQCVEAAERLA
                     GWLQNR"
     gene            complement(4313..7003)
                     /gene="glnD"
                     /locus_tag="AC16VR_07980"
     CDS             complement(4313..7003)
                     /gene="glnD"
                     /locus_tag="AC16VR_07980"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HIR6011134.1"
                     /translation="MSTTERAARSPGSDGNEGSRRFGNADPFLEELKVRLAQADERMA
                     QRYWDRADVDELIAERTTFFDGLLREVWDHALSEDAQKGLALFAVGGYGRREQFPYSD
                     IDLLIVGKRPASHREAISRFLQVLYDLNLEVGHSVRTVRDCKKEAQKDITVATAIFER
                     RFLFGSDALRHRLDRVLSSSRLWPAEAFFRAKRDEQAHRHRQYDNVEYGLEPNVKSSP
                     GGLRDLQTALWVCQRKFGTTDPEALEALGMLTPLEREWLTEGRRYLWWVRFGLHLIAG
                     RKDDHLQFEHQRALAQRLGFADTNARLGVERFMHLYYRHVLELRELNDILLQHFEEAI
                     LSSRGRPRIEPINERFQIHNDYIEATDPTVFERSPRAILEIFVIMANRRDISGVRAQT
                     IRLIRDSLHLIDDSFRADPENAALFMDLLKAPYTLVSQLTRMRRYGVLARYLPEFGRV
                     VGQMQHDLFHIYTVDAHTMMVIGNMRRFRYRASEERFPVACHCVKEIPKPELLYVSGL
                     YHDIGNGRGGDHSELGAGDVTEFCRRHGLNEADTDLVAWLVRQHLLMSSTAQRKDIYD
                     PQVIHDFATEVKSEMRLNYLYALTVADINATNPDLWNSWRATLLRQLYGETRKALRRG
                     LETPLDKATSVNACRESAHERLVAAGMTPEAIEHAWRVPGDEFFLRHSPRQVAEVTQL
                     IHGHDVRSEPLVTLLDLKGQVSQEGATEVLLYTTDRENLFAASVLALSQLDLSIYDAN
                     IHTSADGLCLNTYVVLTAEGKPLPREGDRRGQVVEKLTRSLADTSTLTGSGKRRLPRQ
                     LRQFTLPTEVSLTTPAGSSNSELTIIASDRPGLLATIGLLFAELGLVVISARIATLGE
                     RVEDVFEISSREGEPITDEEAVYLLENTLRQRLDRQVAAS"
     gene            complement(7023..7814)
                     /gene="map"
                     /locus_tag="AC16VR_07985"
     CDS             complement(7023..7814)
                     /gene="map"
                     /locus_tag="AC16VR_07985"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003092395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HIR6011135.1"
                     /translation="MVAKLENEDDIARMRNAGRLAAEVLEMIGEHVRPGASTEDLNGI
                     CHRYIVEEIDCIPAPLNYSNGPGQPAFPKSICTSVNHVVCHGIPAAKKVLKSGDIVNI
                     DVTVIKDGFHGDTSKMFMVGKPSVLAERLVRVTQECLYRGIDAVRAGATLGDIGHAIQ
                     SHAEAQHFSVVREFCGHGIGRVFHDEPQVVHYGRPNTGMRLEEGMTFTIEPMINAGRR
                     HVKILPDHWTVVTKDHKLSAQWEHTILVTATGAEVLTRRSDESLP"
     gene            8059..>8254
                     /gene="rpsB"
                     /locus_tag="AC16VR_07990"
     CDS             8059..>8254
                     /gene="rpsB"
                     /locus_tag="AC16VR_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017130857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HIR6011136.1"
                     /translation="MNISMRDMLAAGVHFGHQTRFWNPKMAPFIFGSRNKIHIINLER
                     TVPAFNEALTELRNLASRRNK"
BASE COUNT         1411 a         2586 c         2729 g         1528 t
ORIGIN      
        1 cccgcaccaa catccgattg gggcgcggat accaggtaga gcaccgtggt tgccagcgtg
       61 taggcgatca gcgcgacgac cagccagcga gcgggttcgg ccaggcgtgt cacgatgaag
      121 ttgagtaggg cggccatgtg tgtcgatact aaaaaacttg ttcttccacc tctagcacta
      181 ccgcgctgtc gggctccgtc tcggtccacg agagattcag gagcctggtc atgcgtgcgg
      241 taacgcctac cttggcaata ccgttgtcga gcagctcggc ggtcaccagc gcagccgccg
      301 ggtcgtcaag cgtcgtgatg gtagcggcgg gtccggggcc cagggtcgcc cgcaggggcg
      361 gcaggatgct tgactgaatg ccattgccgg cgggatagct gatgctgccc ccttcccatt
      421 ccaccactcc atttgcgtcc cgcagcgcaa cgtcgtcgaa tgtcagcagg acgtttgaca
      481 gatccatggt gccggagatg gtcatgtcgt acggctcgag ccacccgttt accgtttggg
      541 cgccgacttt acccgatacg tccagggtaa acgttcgatt caatcccaca acggcggtac
      601 cccgcaactg gtggtgcgca tggctaaggg taaagtcgca gcccagcgcg cccgccacaa
      661 cggttgccgg atggacgtcc cactgaagcc tgcccacggg ctcggagcga acgtacagat
      721 cgcctccacc ctgccaaagg gttccgttca cccgcgtcag gtcgacgccg ttcacccggt
      781 ccaggacgag gccggtgagg ccggccggcg caaagacgat gcagaacgtc agaaagagca
      841 gaaaacctaa cagaataagg gtgcgcatgt tcagccgagc aactgatcga gaacgcgctg
      901 gtagatgtct gccagtggga gcaggtcggc ggtcgccacc cgctcgtcga tcttgtgaat
      961 cgtcgcgttc acagggccca gctcaacgac ctcgcagccc gtgggggcaa tgaagcgacc
     1021 gtctgacgtg ccgccagagg tcgatgcctc cggcgttctg ccggtgacgt cgcccaccgc
     1081 cttattgacc gcggctcgca ggtcgccggg tgctgtgagg aacggctcgc cggacactac
     1141 ccattcgagg tcgtactgaa ggccgtgacg gtcgagtatt tccgccgcct gccggtgcaa
     1201 cccggatgag gtctgctctg tgttgaagcg gatgttgaag tcgaccacca gggatccggg
     1261 aatgacgttg ggcgcgcccg tgcccgcgtg gatgttcgac acctgcagac cggttggcgg
     1321 aaagccatcg tttccttcat cccagcgttt gttggtcagc tcggccagag cgggcagcag
     1381 ggtgtggacg ggattctctg ccagctccgg gtacgcgacg tggccctggg tgccgaatac
     1441 cgtgaggcgc gcgttcaccg agccgcgccg accgacccgt acggtgtcgc ccagaaggtg
     1501 ggccgaggac ggctcgccga ccacgcaaaa gtccatctcc tgtgcgcgct ctgcctgaag
     1561 cgtctccacc acccgccggg tgccgtccac ggccggtcct tcctcgtcgg aagtcagcag
     1621 gtacgcgagc gtgccccggt ggcgggggtg agctgccagg aagcgctcgg tagcgacgac
     1681 catggcggcc aggctggcct tcatgtccgc ggcgccccgc ccccacagaa acccgtcgtg
     1741 cagcgtgggc tcgaacggcg ggttcgtcca tgcgtcctgc ggtccggccg ggacgacgtc
     1801 ggtgtggccg gcgaaaacga atagcgggcc gccctcgcca tgggtggccc agaggttgct
     1861 cacatcaccg aaattcatcg ggcggttcac gaagcccatc gcagcgagcc gctggccgat
     1921 cagctcctgg cagccctcgt cactcggggt cacgctggcc cgccgcatca gctccacggt
     1981 gagttcgaga accggatttt gcgatccttc gctcaatggt ttgtcagttg tgtgcgtgta
     2041 gctgttcgtt gagctcgatg gctttgcggt tgctgcggca ctcgatgcgt cccgatcggc
     2101 cgttgcggat gaacagcaaa tcggagcctc ctgccagttc gcgcgccttc atcgtccgca
     2161 catcttctcc tgtctcgtcg atgagagtga ccggcgtgcc ggccgtgatg tagagcccgg
     2221 cctcgatcgt gcatcgatcg ccgagcggaa tgccggttcc ggcgttggcg ccgatcagac
     2281 agtgcttgcc gatgctgatg gtgatctctc cgcctcctga cagcgtgccc atggtgctgg
     2341 cgctgccgcc gaggtctgaa tgctccccca ggatgacgcc ttgagagatc cggccctcga
     2401 ccatgttggg gccgagggcg ccagcgttga agttgatctg gccctcatgc atgacggtgg
     2461 tgccgtcgcc caggtaggca ccgagtcgta tgcggctggc gtcggcgatt ctcacaccgc
     2521 tgggaacgat gtagttggtc attcgcggga acttgtccac ggccagaacc tcgatgtgcc
     2581 cgccagcgag ccttgcctgc agctgtgcgt ccgccagttc ttccggatcg atgggcccgc
     2641 ggtcggtcca agcgacgttg ggaagatgag cgaatatgcc gtccagattc agcgtgttcg
     2701 gaagggccat gcggtgcgag agcaggtgca gcttcagata cgcggtcgcc gacgagtcga
     2761 tgggctcgtc agcgtcgagc cgaacggccg tcatagcccg gtctgtctca gccaggctgc
     2821 tgagcagccg ggccatggcc gagtcgccga ctgccttggc gaagcgggcg agttcggcgg
     2881 gccctacttc atgagcgccg ctggaaaacg acccttcgat ctgcgccgcc aggtccggtt
     2941 ccgggttgat gacgggggcc gggaagtaga agtctagcgg cgcgccggct gtgtttcttc
     3001 gcaccgtccc gaccgcgaac gcgaatatgc ctgacatgct attgacctcg cgatgcgggt
     3061 ggcgcgtaac ctaccacaac gccgtctcgg ccgttagtac gtaccggcac tagcggttct
     3121 gaagccaccc ggcaagacgc tcggcggcct cgacgcactg tgcttcggag gcgacccagg
     3181 caacccgtac gtgaccaccg ccggggttcg agtcgccgcc ggcccgtccc agaaacgagc
     3241 cgggcagaac ggtcaggttg cactgctcga aaagttgacg ggcgaatgcc gtgtcgtcct
     3301 cgccgatgtg caaccagtgg tagaagccgc cggcgggcgt gcgaaggtcc agatgcgggt
     3361 cgagcaccct gccgaccgtc tcgaactttc gtcggtagag ggcgcggttg gcttccacat
     3421 gagcttcgtc cgaccacgcg gccgcgctgg cctgctggac gtgcaggggc agcgcgcatc
     3481 cttcgtacgt gcggtatttg aagtagccgg cgagaattgc ggcgtcgccg gcgacgaatc
     3541 cggaacgcag cccgggcagg ctggaacgct tggaaaggct gtggaaagca acgcagcggg
     3601 cgtagtcgtc gcgaccggcg cggtgagcga tctccagcag gccggcgggc gggtgtgagt
     3661 cgtccagata gatctccgag tagcactcgt cggcggcgac gacgaagtcg tgccggtcgg
     3721 cctgctcgag cagccagagc acggtgtcct ccggaatggt ctctccggtc gggttgcccg
     3781 gcgagcacag atagacgacc tcgcaagcgc tccagacgga gtccggcacg ccggcgaagt
     3841 ccggctggta gtcgtttgct cggtcgcaat tgacgtagtg cggcacgccg ttgcccagca
     3901 gcgctgcccc ttcgtagatc tggtagaacg ggttaggcat cagcacacgg gcgtcagcct
     3961 tgccgcttag cgccgcgagg ccgaatgaga acagcgcttc ccgagtgccg gcaaccggca
     4021 gaacctgatt gtcgggatcg aggcgaacgc cgtaacgcct cgccagccag ccgctgatgg
     4081 cctctcgcag ctccggaata cccctcgtgg ctggataggc gcccagcccg gcgcgcagcc
     4141 ggtcggggtc gctcaacaga ttgatcacga actccggcgg ggcgtgcttc ggctcgccga
     4201 tggagagcgc gatgtgaggg aactcggcat tgccccggac gccggccaga agttcggcca
     4261 gccgctcgaa ggggtaggcc tgcagctttt ccaggttcgc gttcaccctg ggtcaggaag
     4321 ccgcgacctg ccggtccagg cgttgccgga gtgtgttttc caggaggtag acggcttcct
     4381 cgtccgtgat cggctcgccc tcccggctgc tgatctcgaa cacgtcctcc acacgttcgc
     4441 ccagggtggc gatccgggcg ctgatgacga ccagcccgag ctccgcgaac agcagcccga
     4501 tggtcgcgag caggccaggt cggtcggacg cgatgatggt gagctcggag ttggaagagc
     4561 ccgcgggcgt cgtcagagac acctcggtcg gcaaggtgaa ctggcgaagc tgtcgaggga
     4621 gcctacgttt gccgctgccg gtcagggtcg aggtgtctgc cagcgatcgc gtcagcttct
     4681 cgacgacctg cccgcgccga tctccctcac gaggcagggg cttgccctca gcagtgagca
     4741 ccacgtaggt gttcagacac aacccgtccg ctgaagtgtg aatgtttgcg tcgtagatcg
     4801 agagatccag ctggctcagc gcgagcacgc tcgcggcgaa caggttctcc cgatcagtcg
     4861 tgtagagcag gacctcggtg gcgccttcct gggaaacctg gcccttgagg tcgagcaggg
     4921 taaccagagg ctcgctgcgg acgtcgtgtc cgtgaatgag ctgggtgacc tcggcgacct
     4981 ggcgcgggct gtggcgcagg aagaactcgt cgccgggaac ccgccaggcg tgctcgatgg
     5041 cttccggcgt catgcctgcc gcgacgaggc gctcgtgggc gctctccctg caggcgttca
     5101 cgctggtcgc cttgtccagc ggcgtctcca ggccccgccg cagggctttg cgcgtctccc
     5161 cgtagagctg gcgcagcagc gttgcccgcc agctgttcca aagatcggga ttggtcgcgt
     5221 tgatgtcggc cacggtgagc gcgtaaaggt agttcagacg catctccgat ttgacctctg
     5281 tggcgaaatc atgaatgacc tggggatcgt agatgtcctt tcgctgagcc gtggaggaca
     5341 tgagcaggtg ctgcctgacc agccatgcca caagatcggt atctgcttcg ttcagcccgt
     5401 ggcggcgaca gaactcggtg acgtcgccgg cgccgagctc cgagtggtcg ccgccccgcc
     5461 cgttgccgat gtcgtggtag aggcccgaca cgtacaggag ttccggcttg ggtatttcct
     5521 tcacgcagtg acaggcgacc ggaaagcgct cttcgctcgc gcgatagcgg aaccggcgca
     5581 tgttgccgat caccatcatc gtgtgcgcgt ccacggtgta gatgtggaag aggtcgtgct
     5641 gcatttgccc gactacgcgt ccgaactccg gaaggtagcg cgccagcacc ccgtaccggc
     5701 gcatcctcgt cagctgcgag accagcgtgt acggcgcttt gagcagatcc atgaacagcg
     5761 ccgcgttctc cgggtcggcg cggaacgagt cgtcgatgag gtgaaggctg tcccggatca
     5821 ggcggatggt ttgcgcgcgc acccctgaga tgtcccgccg attggccatg atgacgaaga
     5881 tttccaggat ggctcggggc gatcgctcga acaccgtggg gtcggtggct tcgatgtagt
     5941 cgttgtgaat ctgaaaacgc tcgttgatgg gctcgatgcg cggccggccg cggctgctca
     6001 ggatcgcttc ctcgaagtgc tggagcagta tgtcgttgag ctcgcgaagc tcgagcacgt
     6061 gacggtagta caggtgcatg aaccgctcta cgcccaggcg ggcgttcgtg tcggcaaatc
     6121 ccagccgctg cgcgagagcg cgctggtgct cgaactgcag gtggtcgtcc ttgcgcccgg
     6181 cgatgagatg aaggccaaac cgcacccacc acagatagcg gcggccttcc gtcagccact
     6241 cgcgttccag gggggtgagc atgccaagcg cctccagagc ttcaggatcg gtggtgccga
     6301 acttgcgctg gcacacccac agcgccgtct gcagatccct cagcccgccg ggcgacgatt
     6361 tgacgttggg ttcgagaccg tactcgacgt tgtcgtactg ccggtggcgg tgcgcctgct
     6421 cgtcgcgctt ggcgcggaag aaagcctccg ccggccacag gcgagatgac gacagcaccc
     6481 gatcgagccg gtgccgcagc gcgtcggacc cgaacagaaa acgccgctcg aagatggccg
     6541 ttgccacggt gatgtccttc tgcgcctctt tcttgcagtc ccgaaccgtg cgaacgctgt
     6601 gcccgacctc caggttcagg tcgtagagca cctgaagaaa ccggctgatg gcctcgcggt
     6661 ggctggccgg ccgcttgccg acgatgagga ggtcgatgtc cgagtagggg aactgctctc
     6721 tgcggccgta gccgcccacg gcgaagaggg cgagcccctt ttgcgcatct tcgctcaggg
     6781 cgtggtccca cacctcgcgc agcaggccgt cgaagaacgt cgtgcgctct gcgatcagct
     6841 catcgacgtc ggcacggtcc cagtatcttt gggccatacg ttcatcggcc tgagcaagac
     6901 gaaccttcag ctcttcgaga aagggatccg cgttgccgaa acggcggctg ccctcgttcc
     6961 cgtcgctgcc cggagatcgg gccgctctct ctgtcgtgct catgacgacg tggccttcgt
     7021 gatcaaggca acgattcgtc gctgcgacgg gtgagcacct cggcacccgt tgcggtgact
     7081 aagatggtgt gttcccactg cgcagaaagc ttgtggtcct tggttaccac ggtccagtga
     7141 tcgggcagga tcttcacgtg acgtctgccc gcattgatca tgggttcgat ggtgaaggtc
     7201 atgccttcct ccaggcgcat tcccgtgttc gggcggccat agtgcacgac ctggggttcg
     7261 tcgtgaaaaa cccggccgat cccgtggcca cagaactcgc gcacgacgga gaagtgctga
     7321 gcttcagcat ggctctggat cgcgtgaccg atatcgccca gcgtcgcgcc cgcgcgcacc
     7381 gcgtcgatgc cccggtacag gcactcctgg gtcacgcgga ccagccgctc tgccaagacg
     7441 ctcggctttc ccaccatgaa catcttgctg gtgtcgccgt ggaagccgtc cttgatgaca
     7501 gtgacgtcga tgttgacgat gtctccgctc ttgagcacct tcttcgctgc cggaatcccg
     7561 tgacagacga cgtgattgac cgacgtgcag atggatttcg gaaacgcggg ctgaccgggc
     7621 ccgttggagt agttcagcgg cgccgggata cagtcgatct cctccacgat gtagcgatga
     7681 cagatcccgt tgagatcctc ggtggaggcc ccggggcgaa cgtgctcgcc gatcatctcc
     7741 agcacctcgg cggcaagccg ccccgcgttg cgcattcttg cgatgtcgtc ctcgttctcg
     7801 agcttggcga ccatgaatct acgtctggct tgggcttgtt taagcggtgg ggcgattatg
     7861 ttataaagcg cgccgcctgc gggcaacgca agccgcccaa cttcggaaga aatatctgcg
     7921 ggaggggggc ggcgggctcc aggcgactaa acgtcacttt gacacaaatg actcacgtcg
     7981 tccgacacgt gtctccgggt gcccgttcga tcgggttgag gcatggggat ggcggaggcg
     8041 caacccggag tacaggatat gaatatcagc atgcgtgaca tgttggccgc tggcgtgcat
     8101 ttcggccatc agacccgttt ctggaacccc aagatggctc ccttcatctt cgggtcccgt
     8161 aacaagatcc acatcatcaa tctggagaga accgtcccgg cgttcaacga ggcgctgacg
     8221 gagctccgaa acctcgccag ccgtcgcaac aaga
//
LOCUS       DBNHQR010000357         2930 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_667628, whole genome shotgun sequence.
ACCESSION   DBNHQR010000357 DBNHQR010000000
VERSION     DBNHQR010000357.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_667628"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1322
                     /gene="nhaA"
                     /locus_tag="AC16VR_07995"
     CDS             <1..1322
                     /gene="nhaA"
                     /locus_tag="AC16VR_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HIR6011137.1"
                     /translation="GTDLPTVVVDRFTGPLARFLKVEAATGVLLLLAAVAALGLANSP
                     WSVSFLAFWETPIGLHFGPFDFSRSLRHWINDGLMTFFFFVIALELKRALILGELRDL
                     RVASLPLAGALGGMIVPASLYFVLMSGRPGANGWGTVMATDTAFVIGCLALFGSRIPA
                     SLRVFAVSVAVFDDVGAILIVAAVYGGALNLNALTLGMLGVVAVAVCARLGMRSIPLY
                     FLLGGAVWLCFDASGIHATMAGVVLGLMTPTRVWVSDTRLRGVLGRALSHPEGEHWSG
                     DSVERSDLLRAGTAVRESLSPLERLEMMLHPWAGFLIMPLFALANAGIAISGADIGQP
                     VSVAIFAALVFGKPVGVLFFSGIAVRLGFAIRPLGLDWPLVAACALLTGIGFTMSLFI
                     AGLAYPPAMLNAAKVGVLTGSLVSALGGLLALLWLTFRRRAAQEAEL"
     gene            2292..2390
                     /locus_tag="AC16VR_08000"
     CDS             2292..2390
                     /locus_tag="AC16VR_08000"
                     /inference="COORDINATES: protein motif:HMM:NF014950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR6011138.1"
                     /translation="MKSCGNKSSAARLLGISRDTLRYRMEKHGFGA"
     gene            2482..2862
                     /locus_tag="AC16VR_08005"
     CDS             2482..2862
                     /locus_tag="AC16VR_08005"
                     /inference="COORDINATES: protein motif:HMM:NF016149.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0046688 - response to copper ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HIR6011139.1"
                     /translation="MKKLISTALGGSLLLVLAAASAPPETGHFGLSRSSPEAGSTVEA
                     PGEVRLWFTQVPQDGTVQVRIVEAADAGVHVMDAVQDGEEPQVFYVEVHGSLPPATYT
                     VSWRGMGEDGHVVRDIFEFTVATR"
BASE COUNT          444 a          964 c          949 g          573 t
ORIGIN      
        1 gcggaaccga cctccctacc gtcgtcgtgg accgattcac cggccccctc gcgcgtttcc
       61 taaaggtgga ggccgcgacc ggcgttctgt tgctcctcgc cgcggtcgcc gcactgggcc
      121 tggcgaactc accgtggtcc gtatcctttc tggcattttg ggagacgccc atcggcctcc
      181 acttcggccc gttcgatttc agccgctcgt tgcggcactg gatcaatgac gggttgatga
      241 cgttcttctt cttcgtcatc gctctggagc tcaagcgggc gctgattctc ggcgagcttc
      301 gtgatctgcg cgtggcgtcg cttccgctcg ccggagcact cggaggcatg atcgtaccgg
      361 cgagcctgta tttcgtgctc atgagcggcc ggcccggcgc gaacggctgg ggcacggtga
      421 tggcgaccga caccgccttc gtcatcggct gcctggcgct cttcggctct cgcatcccgg
      481 cgagcctgcg cgtgttcgcc gtgtcggtgg cggtgttcga tgacgtgggt gccatcctga
      541 tcgtagccgc ggtctacggg ggtgccctga acttgaacgc tctgaccctc gggatgctcg
      601 gagtcgtggc ggttgcggtc tgtgcccgcc tggggatgag gagtattccc ctctacttcc
      661 tccttggagg cgcggtgtgg ctttgcttcg atgcttccgg catccatgcg acgatggctg
      721 gcgtcgttct gggtctgatg acgccgacac gcgtatgggt gagcgacaca cgtctccgag
      781 gcgtcctcgg ccgcgccctt tcccatccgg agggtgagca ctggagcggg gactctgtgg
      841 agcgaagcga cctgctgcga gccggcactg ccgtcaggga gtcgctcagc ccgttggaac
      901 ggctggaaat gatgctccac ccatgggccg gattcctgat catgccattg ttcgctctgg
      961 ccaatgccgg catcgcgatc tccggtgccg acatcggaca gcccgtctcc gtggcgatct
     1021 tcgccgcact ggtcttcggc aagccggtcg gcgtcctgtt cttcagtggg atcgccgtac
     1081 gcctcggctt cgccatccgc ccgcttggct tggactggcc cttggtggcg gcctgcgccc
     1141 tcctgaccgg gatcggcttc accatgtcgc tgttcatcgc gggcctggcc taccccccag
     1201 cgatgctcaa cgctgccaaa gtcggcgttc tcacggggtc gctggtatcc gccttgggcg
     1261 gtctccttgc gctactctgg ctgacgtttc ggagacgggc cgcacaagaa gcggaactct
     1321 gaagcgccct ttgcgctgga gcggtcgctc acccacgacg cgggtaagta cctgggcctc
     1381 gaatgggcag gcaagcgttg cggcgggcct gaatagcgcg ccagcgaggg cccgacaccg
     1441 tgttcgcgaa gcgcctgtcg gcgaggcccg cagctgaaat ctacccaggc ccactgaagc
     1501 tcccccgatc actgaccgag agcttcttca tgttcgtcgg ctaccgacct cgcgaccgcg
     1561 cagcgggctg tgtgccatat caggatcgcc cgccctgagt cggcaagagc gcgacttgcc
     1621 tgcgacgatg cagtgcatct agtctgcgcg tccgttcgca cggatgcagc tgtgacacaa
     1681 cctgcaccag cgcccaccgt cggtggctgt cgctgaccgg cgtgcagagt gcgaaagccc
     1741 aatgattcca gcgcccacca gcgcagaccc gcgccccggc ggattctgat aagcgtgagg
     1801 tcggtagttc aaatctaccc aggcccactg agcccgctcg gtcacgacgg ccgggcgggc
     1861 ttcttcatgc cataacgtcg agaactaccg acctcacggg tgcactccgg tgactcggta
     1921 gttcactcgg aggcgcgtag cgccggagag gagcgagggc cggaccggcg gccgcgaagc
     1981 gcccgccccg ggtgatccgc agctcaaatc tacccaggcc tactcagccc gcccggtcga
     2041 aagacctggc gggcttttcc atgctacccg gtccagaact accgacctca cgggaccacc
     2101 agttgactcg gtagttcacc cggagcgcgc ggtcggcgcg cggagggtag cgagggtctg
     2161 aaaggggttt gcgacagcgc ctgcccgcga gccccgcagc tcaaatctac ccaggcccac
     2221 tgtgaagccc gtccggtccc ttcgacctgg cgggtttcgc cgtccaagag gttgggtttg
     2281 accagacgct tgtgaagtcg tgtggcaaca agagcagcgc cgctcgcctc ctcggtatct
     2341 cgcgtgatac gctccgctat cgaatggaga agcacggatt cggtgcctga gtggggcttt
     2401 gctgggctcg gaccctttcg cggcgaccga tgtacttcgt gcacgatcga ccgtagccct
     2461 aaccaggata gtgacacgac cgtgaagaag ctgatctcta ccgcgcttgg cggctccctc
     2521 ctcctcgtgc ttgcggccgc gagtgcgcct cccgagaccg gtcacttcgg cctgagcagg
     2581 tcctcacccg aagcggggtc caccgtggag gctcctggag aagttcgcct ctggttcacg
     2641 caggtgccac aggatggcac ggtccaggtc cgaatcgtcg aggctgcgga tgccggagtc
     2701 cacgtcatgg atgccgtcca ggacggcgag gaaccccagg tcttctacgt ggaggtgcat
     2761 ggctcgcttc ctccagcgac ctatacggta tcgtggcgcg gaatgggcga agacggtcac
     2821 gtcgtgcgcg acattttcga gttcaccgtg gccactcggt agcgcggacg cgccaatgca
     2881 aggggtggcg ccgcccagtg aggccaggga gtgattctat ccggattgag
//
LOCUS       DBNHQR010000358         3601 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1173077, whole genome shotgun sequence.
ACCESSION   DBNHQR010000358 DBNHQR010000000
VERSION     DBNHQR010000358.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1173077"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="AC16VR_08010"
     CDS             <1..273
                     /locus_tag="AC16VR_08010"
                     /inference="COORDINATES: protein motif:HMM:NF017984.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase AlkZ-like family protein"
                     /protein_id="HIR6011140.1"
                     /translation="PAGKRRYGYFSLPILFGDELVGRMDSKADRRTGTLTVNLLHLEP
                     GADPAAIRDPLMQALNGFARDNGCTRVIMGATEPASLKAELARVLG"
     gene            complement(305..1801)
                     /locus_tag="AC16VR_08015"
     CDS             complement(305..1801)
                     /locus_tag="AC16VR_08015"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6011141.1"
                     /translation="MTDLVRKGWTVLALWALTMILGYFGLGGFLAETVEGIGGVPAWP
                     TALFVVLLAVSLWASVVWARGLKAKPEAGAALEERPLASGRRRFLTGTAAVTGGMLGA
                     AGATIGRVAPWATVTLPALRQPVTVTDPNPKDDWLGARVRSYRPLGDTGLMVSDIATG
                     STRIHHNSDPVGYLKSLLDRGVNYIDASPDYAPESEGIIAEAIVGRPRDQLVIASKFC
                     TRDGHLRQGSSVAAYMTAIDGTLSRMGTDYIDVAHIHSCDSTERLMDENVHEAFDRLK
                     DAGKVRHLGVSTHTPNLEEVADTAIDSGRFKVMMLAYHHGAWPKQMDIIDRAAAAGMG
                     IVAMKTLKGAKHRGLAEFRPEADSYAQAAFKWVLANPSVSNLVVSFYDNQHPDEYLFA
                     SGKSLERSDIAVLGKYDQLIAGTHCFQHCGDCLGACSSGLAINDILRHRMYFEDYGDE
                     KQAMQLYAKLDRQADVCIDCSAPCLGSCPQGVEIQERMIGAHEKLRWA"
     gene            complement(1901..>3601)
                     /locus_tag="AC16VR_08020"
     CDS             complement(1901..>3601)
                     /locus_tag="AC16VR_08020"
                     /inference="COORDINATES: protein motif:HMM:NF012946.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6011142.1"
                     /translation="EQAKYRAFKEVRRGAADYMTMEGEFARYLEDVYSADPVPREALT
                     DECEVLVVGAGFAGLLLWQKLSAAGFTDVRFCEKGGDVGGTWYWNRYPGIACDVESYS
                     YLPLLEEMGYIPSMKFASGFEILEYCQRMAEKFGFYDRCLFHTTVDRTEWDEAAGRWT
                     VYTNRGDAMRARFVVLANGILTTPQLARIDGMETFKGESFHTSRWNYHVDLAGKRVGI
                     IGTGATAVQVIPEIAKIVKELYVFQRTPSSIDVRDQRETTPEEYETWSHEPGWARARR
                     ARFSKISSGRTAIKANDDYLSGKVADFKERKQHERRLSAEEMINKQLETNFRIMEQIR
                     ARVDAIVEDPRTAEALKPYYPYGCKRPTFHDEYLPTFNLPHVTLVDTAPLGVTRINER
                     GVVHDGREYPLDVLIYATGFQWMATSTFNMIRGRGGRTLNEKWQEEGTRTFLGIHSHG
                     FPNLFIVSGPQGGGGSFNFTDAIDTHADYICWMLETMRDRGARTVDVRKEPEDEYAEH
                     CRAADVMTAPLRDCISYYNGHGDAEPGSLAYYGGGRWHKYRVRAQESLEPYDFDDIPE
                     "
BASE COUNT          614 a         1180 c         1155 g          652 t
ORIGIN      
        1 cccgcgggca agcggcgcta tggctatttc agcctgccga tcctgtttgg cgacgagctc
       61 gtcgggcgga tggacagcaa ggcggatcgg cgcacgggca ccctgacggt gaacctgctc
      121 cacctcgaac ccggcgcgga tcccgctgcg atccgagacc ccttgatgca ggcactgaac
      181 gggtttgccc gggacaacgg ctgcacccgg gtcatcatgg gcgcaaccga acccgcatcg
      241 ctgaaagccg agcttgctcg cgtcctcggc tgaccggggc gaaggcccaa tcggctacaa
      301 ggtctcaggc ccagcgcagc ttctcgtggg cgccgatcat gcgctcctga atctcgaccc
      361 cctggggaca gctgccgagg cagggtgctg agcagtcgat gcagacgtcc gcctgccggt
      421 ccagcttggc gtaaagctgc atggcctgct tttcgtcccc gtaatcctcg aagtacatgc
      481 gatgacgcag gatgtcgttg atggcgagcc cactcgaaca cgccccgagg caatcgccgc
      541 aatgctgaaa gcagtgggtg cccgcaatga gctggtcgta tttgccgagc accgcgatgt
      601 cgctccgctc gagggacttg cccgaggcga acaggtactc gtccggatgc tggttgtcgt
      661 aaaacgaaac caccaggttg gaaacggacg ggttggccag cacccacttg aaggccgcct
      721 gggcgtagga gtccgcttcg ggcctgaatt ccgcaaggcc gcggtgcttg gcgcccttga
      781 gcgtcttcat ggcgacgatg cccatgcccg ccgcggccgc gcgatcgatg atgtccatct
      841 gcttcggcca ggcgccgtgg tggtaggcca gcatcatcac cttgaaacgg ccgctgtcga
      901 tggcggtgtc ggcgacctcc tctaagttgg gcgtgtgggt agacactccc agatggcgca
      961 ccttgccggc atccttgagg cgatcgaaag cctcgtggac gttctcgtcc atgagccttt
     1021 cggtgctgtc gcaggaatgg atgtgggcga cgtcgatgta gtccgtgccc atccggctga
     1081 gcgtcccgtc gatggcggtc atgtaagctg cgacgctcga gccctgccgg agatggccgt
     1141 cgcgagtgca gaacttggag gcgatgacca gctggtcccg gggccgcccg acaatggcct
     1201 cggcgatgat gccctcgctc tccggggcgt agtcgggtga ggcgtcgatg tagttgaccc
     1261 cgcggtccag aaggctcttc aggtacccga ccggatccga gttgtggtga atgcgcgtgg
     1321 agccggtggc aatgtcggag accatcaatc cggtgtctcc caaaggccgg tagctgcgca
     1381 ccctggcgcc cagccagtcg tccttcgggt tcggatccgt gacggtcacg ggctggcgca
     1441 gggcaggaag cgtcaccgtc gcccagggtg ccaccctgcc gatggtcgcc cccgcggccc
     1501 cgagcatgcc gccggtcacc gccgcggtac ccgtgagaaa acgacgtcgc ccgctggcga
     1561 gtggcctttc ttccagcgcg gcccccgctt cgggcttcgc tttcagcccg cgcgcccaca
     1621 ccacgctggc ccagagactc acggccaaga ggaccacgaa tagcgccgtg ggccaagcgg
     1681 gtaccccacc gataccttca accgtctcgg cgagaaagcc gccgagaccg aagtagccga
     1741 gaatcatggt caaggcccaa agggccagaa cggtccatcc tttgcgaacg agatcggtca
     1801 tcatcccctc tccaggccgg ctaggcctga actatatcga cagccgcggg gggatcaaac
     1861 cgcgtccgtt gcagtactcg ccgatcccca ggggtttcca ctactccgga atatcgtcga
     1921 aatcgtaggg ttccagactc tcctgagcgc gaacgcgata cttgtgccag cggccgccgc
     1981 cgtaataggc aaggctgcca ggctccgcgt ccccgtgacc gttgtagtag gaaatgcagt
     2041 cgcgcagggg ggccgtcatg acgtccgccg cccgacagtg ctcggcatac tcgtcttccg
     2101 gttccttgcg cacgtctacc gtacgggcgc cccggtcgcg catggtctcc agcatccagc
     2161 agatgtagtc ggcgtgggtg tcgatggcat cggtgaagtt gaagctgccg ccgccgccct
     2221 ggggacccga gacgatgaag aggttcggaa agccatgact gtgtatcccg agaaaggtgc
     2281 gggtcccctc ctcctgccac ttctcgttga gcgtgcgccc accccgtcca cggatcatgt
     2341 tgaacgtgga cgtcgccatc cactggaagc cggtggcgta gatcagcacg tccagggggt
     2401 actcccggcc gtcgtgcacg acgccacgct cgttgatcct ggtgaccccc agcggcgcgg
     2461 tgtccaccag ggtgacgtga ggcaggttga aggtgggcag gtactcgtca tggaacgtcg
     2521 gccgcttgca cccgtacggg tagtagggtt tcagcgcctc ggccgtcctg gggtcctcga
     2581 cgatggcgtc cacgcgggcg cggatctgct ccatgatgcg gaagttggtc tcgagctgct
     2641 tgttgatcat ctcctcggcg gagagcctgc gctcgtgctg cttgcgctcc ttgaagtcgg
     2701 ccacctttcc cgacaggtag tcgtcgttgg ccttgatggc cgtccgcccg gaggagatct
     2761 tcgagaatcg cgcgcgccgc gcccgggccc agccgggttc gtgcgaccag gtctcgtact
     2821 cttccggcgt ggtctcccgc tgatcgcgca cgtcgatgga cgacggcgtc cgctgaaaga
     2881 cgtagagctc cttcacgatc ttggcaatct cggggatcac ctgaacggcc gtggcgccgg
     2941 tgccgataat gcccacccgt ttgccggcca gatccacgtg atagttccag cgtgatgtgt
     3001 ggaaggactc gcccttaaac gtctccatac cgtcgatacg cgcgagctgg ggcgtggtga
     3061 ggatgccgtt ggccagcacc acgaagcgcg cgcgcatggc gtcgccgcga ttcgtgtaaa
     3121 ccgtccagcg accggccgcc tcatcccact ccgtgcgatc cacggtggtg tgaaacaggc
     3181 agcgatcgta gaagccgaac ttttccgcca tccgctggca gtactcgagg atctcgaaac
     3241 ccgaggcgaa cttcatgctg gggatgtaac ccatctcctc cagcagcggg aggtagctgt
     3301 aggactcgac gtcgcaggcg atgcccgggt agcggttcca gtaccaggtg ccgcccacgt
     3361 cgccgccctt ctcgcagaag cgcacgtcgg tgaatccggc tgcggaaagc ttctgccaaa
     3421 ggagcagacc ggcgaaaccc gccccgacca cgagcacctc gcactcgtcg gtcaacgctt
     3481 cccggggcac cggatccgcg gaatagacgt cctctaagta cctcgcgaac tcgccttcca
     3541 tggtcatgta gtcggcggcc ccgcgacgga cctccttgaa ggcccggtac ttcgcctgct
     3601 c
//
LOCUS       DBNHQR010000359         4521 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1173124, whole genome shotgun sequence.
ACCESSION   DBNHQR010000359 DBNHQR010000000
VERSION     DBNHQR010000359.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4521)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1173124"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="AC16VR_08025"
     CDS             complement(<1..466)
                     /locus_tag="AC16VR_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017848940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6011143.1"
                     /translation="MQFPESFDVIVIGAGHAGAEAAAAAARVGARTLLLTQSIETIGQ
                     MSCNPAIGGIGKSHLVREIDALDGIMAKAADRAGIQFRILNSSKGPAVRATRAQADRA
                     LYRQAIRELLEAQENLYLFQQSVVDLIVEDGSVRGVVTQIGLEFRAPCVVLTT"
     gene            complement(540..1877)
                     /gene="mnmE"
                     /locus_tag="AC16VR_08030"
     CDS             complement(540..1877)
                     /gene="mnmE"
                     /locus_tag="AC16VR_08030"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007160130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HIR6011144.1"
                     /translation="MDVDTIAAVATPVGSGGVGIVRISGPRARAIGETVSARSLHPRE
                     AFYCRFRNAQGEVVDDGIVLYFAGPASFTGEDVVELQGHGGPVVVQMVLGIVLEAGAR
                     QARPGEFSERAYLNGKMDLAQAEAIADLISSSSTAAARGAVRSLQGVFSERVRKIDEA
                     VLGVRMYVEAAIDFPEEEVDFLSEGDIAARITAILADVDDLIRDSRQGVLLRDGVTIA
                     LLGAPNVGKSSLLNALAGEDRAIVTDIPGTTRDLVRADLELAGIPVTVVDTAGLRTTD
                     DPVEREGVRRAEDQARSADLILVVTDVTDPNECSIELPEASQEIPVIQVHNKIDRTSA
                     PPGPLTGEPLPVVRVSALTGDGIDELRAQILAAVGYTEHAGTFTARQRHLLALQAAAA
                     GLARAAGLAASADGGELVAEELRGVHTELGTLLGGITADDLLGEIFSSFCIGK"
     gene            complement(1888..3618)
                     /gene="yidC"
                     /locus_tag="AC16VR_08035"
     CDS             complement(1888..3618)
                     /gene="yidC"
                     /locus_tag="AC16VR_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HIR6011145.1"
                     /translation="MLPTEVLRIVLLIALGVVGYLLILQWNEDYMQRPAAPDYAAEPS
                     PSAAEPALTAAPDLPVGAELPAGTGPSDIPDESLIGQDGAETQPALSVPMPDIGERLV
                     VVRTPLLHVWIDRLGGDVVRVALPKFPVDLDRPDVPFVLLDNGLDRTYVAQSGLIGPD
                     GIDAGGDRPLYATQAQSYTLEAGELVVELSTLRDGLEVAKRYMFRSDDYLMEVSYQVR
                     NLTGRTISARMFGQLKRDRKEPYVDTSIALGPQPYLGAAFTTAENRYEKLDFDDLDEG
                     RFRTTEQGGWVAFLQHYFLSAWIANPEETNSYYGQRRPDGTYMFGFSSPVSQVASGAL
                     GEWAASLYVGPKDQKRLEEIAENLNLTVDYGFLWWLAVPLFYLLDWLHGFVGNWGVAI
                     ILLTLLVKLVLYPLSATSYKSMANMRRVAPQMKRLQERHADDRQKLSQEMMALYKKEK
                     VNPLGGCLPMLLPMPIFIALYWVLFESVELRQAPFALWINDLAAMDKYFVLPLLMGAS
                     MYLQQLLSPAVGDPMQVRMMRLMPIMFTVLFLFFPAGLVLYWLVNSVLSIAQQWYVIR
                     QTERAYAAKS"
     gene            complement(3625..3864)
                     /gene="yidD"
                     /locus_tag="AC16VR_08040"
     CDS             complement(3625..3864)
                     /gene="yidD"
                     /locus_tag="AC16VR_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003156579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HIR6011146.1"
                     /translation="MLRNAPRRLLQAGIRLYQWTLSPYLGRQCRFYPTCSHYAHEAIE
                     EFGAVRGSVLAARRLGRCHPFHPGGVDLVPTQEEG"
     gene            complement(3864..4286)
                     /gene="rnpA"
                     /locus_tag="AC16VR_08045"
     CDS             complement(3864..4286)
                     /gene="rnpA"
                     /locus_tag="AC16VR_08045"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HIR6011147.1"
                     /translation="MAARSSTRGAPKVANASPSEAGDRAAASIHSFPRRRRLTRSRDF
                     DRVLKNAEVRRRSGPLRLTAVANTMQGARLGMIVGKRAVGRAHERNRMKRVIRDEFRR
                     RHAELADMDMVILVMAELENRELRAHLARLFESEVQKR"
     gene            complement(4228..4362)
                     /gene="rpmH"
                     /locus_tag="AC16VR_08050"
     CDS             complement(4228..4362)
                     /gene="rpmH"
                     /locus_tag="AC16VR_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HIR6011148.1"
                     /translation="MKRTFQPSNLKRKRTHGFRSRMSTRGGRQVINARRAKGRKRLSV
                     "
BASE COUNT          850 a         1449 c         1410 g          812 t
ORIGIN      
        1 ccgtcgtcag cacaacacag ggcgcccgga attccaggcc gatctgggtg accacaccac
       61 ggaccgagcc gtcttcgaca atcagatcca ccaccgactg ctggaagagg taaaggttct
      121 cctgggcctc taacagctcc cggatggctt gtcggtaaag ggccctgtcc gcctgggcgc
      181 gggtggccct gacggctggc cccttgctgg agttgaggat tcggaactgt atgccggcgc
      241 gatcggcggc tttggccatg atcccgtcca gcgcgtcaat ctcccggacg aggtgggact
      301 tgcctattcc accaatggcc ggattgcacg acatctgccc gatggtttcg atgctctggg
      361 tgagcagcag agtccgcgcc cccacccggg ctgccgcagc ggcagcttcg gcgccggcat
      421 ggccggcacc aatgacgatc acgtcgaacg actcgggaaa ctgcaaagct aatatccgcc
      481 aggttgcaag cccgcgaagt ataagtactc ggattccgat tggaagggcg cggccggggc
      541 tacttgccga tgcagaagct cgagaaaatt tcgccgagca ggtcgtcggc cgtaatgccg
      601 cccagcagag tgccgagttc ggtgtgcacg ccgcgaagct cttccgccac gagctcgccc
      661 ccgtcggccg aagcagccag accagcggct cgcgccaggc ccgcagcagc agcttgcagc
      721 gccagcaggt gcctttgccg ggcagtgaag gttcccgcgt gctcggtgta gccgacggca
      781 gcgagaatct gggcgcgaag ctcgtcgata ccgtctccgg tgagcgcaga aaccctgacc
      841 acgggcaggg gttcgcccgt caatggccca ggtggcgcgc ttgtccggtc aatcttgttg
      901 tgcacctgaa tgacggggat ctcctgcgac gcttcgggca gctcaatcga acattcgttc
      961 gggtcggtga cgtcggtgac caccaggatc agatctgccg agcgcgcctg atcctctgcc
     1021 cgccgaacgc cttcgcgttc caccgggtca tcggtggttc gcaaacccgc cgtgtccacc
     1081 accgtgaccg gtatgcctgc gagctcgagg tcggcgcgca cgagatcgcg cgtggtcccg
     1141 ggaatgtcgg tcacgatggc ccggtcctct ccggccagcg cgttcagcag actcgactta
     1201 cccacgttgg gcgcgccgag aagcgctatg gtgactccgt cccgaagcag aacgccctgc
     1261 cgggaatccc ggatcagatc gtcgacgtca gccaggatgg ccgtgatccg ggcagcaatg
     1321 tcgccttcgc tgagaaaatc cacttcttcc tccggaaagt cgatggccgc ttccacgtac
     1381 atccgcacac cgagtaccgc ttcatctatc ttccggaccc tctcggagaa aacgccctgg
     1441 aggctgcgca cggccccgcg agcggctgcc gtggacgagc tggaaatcag atcggcaatg
     1501 gcctcggcct gagcaagatc catcttgccg ttcagatagg cccgttcgga gaactccccg
     1561 gggcgtgcct gccgggcccc tgcctccagc acgataccaa ggaccatctg cacgactacc
     1621 ggcccgccgt gcccctgcag ctccaccacg tcctctcctg taaaggaggc cggtccggca
     1681 aagtagagaa cgatgccgtc atcgacgacc tcaccctggg cgtttcggaa cctgcaatag
     1741 aaggcctcgc gtgggtggag gctacgggcc gagaccgtct ctccgatggc gcgggctcgg
     1801 ggcccggaga ttcgcacgat gcccacgccc ccggaaccga caggcgtcgc cacggccgcg
     1861 atcgtgtcga cgtccaggcc gagagagtca gcttttggcg gcgtaggcac gctccgtctg
     1921 ccttatgacg taccactgct gggcgatgga cagcacgctg ttgacgagcc agtagagcac
     1981 cagcccggcc gggaagaaga gaaacagcac ggtgaacatg atgggcatga gccgcatcat
     2041 gcggacctgc atgggatcac cgaccgcggg gctcagcagc tgctgcagat acatgctcgc
     2101 gcccatcaga agaggcagga cgaaatactt gtccatggcc gcgaggtcgt tgatccagag
     2161 ggcaaagggc gcctgtctga gctccacact ctcgaaaagc acccagtaca gagcgatgaa
     2221 tatgggcatg ggcaggagca tgggaaggca tccacccaac gggttgacct tctctttctt
     2281 atatagggcc atcatctcct gggacagctt ctgacggtcg tccgcatgcc tctcctgcaa
     2341 ccgtttcatc tgaggggcaa cgcgccgcat gttcgccatg gacttgtagc tcgttgccga
     2401 gagcgggtag agcaccagct tgaccagcag ggtgagcaga atgatggcga ctccccagtt
     2461 cccgacgaaa ccgtgcagcc agtcgaggag gtagaagaga gggactgcca accaccagag
     2521 aaacccgtag tccacggtga gattaagatt ttcagcgatc tcttccaggc gcttctgatc
     2581 cttggggccc acatacaggc tggccgccca ctcgcccaag gccccgctgg ccacctggct
     2641 aaccgggctg gaaaatccga acatgtaggt gccatcgggt cggcgttgac cgtagtaact
     2701 gttggtttcc tccgggttgg ctatccaggc gctcaagaag tagtgctgca ggaaggccac
     2761 ccaaccgccc tgctcggtgg tccggaagcg gccttcgtcg agatcgtcga aatcgagctt
     2821 ctcgtagcgg ttctctgcgg tggtgaacgc agcccccaga tagggctggg gtccgagagc
     2881 aatcgaggta tccacgtacg gctctttgcg atcccgtttg agctggccga acatccgagc
     2941 cgagatggtg cgcccggtca ggtttctaac ctggtaggac acttccatga ggtagtcgtc
     3001 gcttctgaac atgtagcgct tggcgacctc gagaccgtcc cggagcgtcg agagctccac
     3061 tacgagctcc cccgcctcca gggtgtagct ctgagcctgg gtggcataca gcggtcgatc
     3121 gccgcccgca tcgatgccgt ccgggccgat gagcccgctc tgcgccacat aggtgcgatc
     3181 gagcccgttg tcgagcagca cgaacgggac gtcgggccga tccaggtcca cgggaaactt
     3241 gggcaacgcg acgcgcacca cgtccccgcc cagccggtcg atccagacgt gcagcaacgg
     3301 cgtcctgacg acgacgagcc gttccccgat gtccggcatc ggcacgctga gggccggctg
     3361 ggtttcagca ccgtcttgac cgatcaacga ttcgtccggg atgtcggatg gtcccgtacc
     3421 cgcagggagt tccgcgccaa caggcaaatc cggcgcggcc gtcagcgcag gctccgcagc
     3481 ggatggcgag ggttcggccg cgtagtcggg agcagcagga cgctgcatgt aatcctcgtt
     3541 ccactgcagg atgagcaggt aacccaccac ccccagcgcg atgaggagaa cgatcctgag
     3601 gacttccgtt ggcagcatgg ctgatcaacc ctcctcttgg gtcggaacca gatcgacgcc
     3661 gccgggatga aacgggtggc agcgccccag ccttcgcgcg gcaaggacgg agccccggac
     3721 ggcgccgaac tcttcaatgg cctcgtgggc gtagtgagag caggtggggt agaagcggca
     3781 ctggcgcccc aggtacggac tcaacgtcca ctgatacaga cggatgcccg cctgaagcaa
     3841 gcggcgcggt gcgttgcgaa gcatcatctt ttttgcacct ccgactcaaa cagcctcgca
     3901 agatgggcgc gaagctctcg gttctccagc tcagccatca ccagaatgac catgtccatg
     3961 tcggccagct ctgcgtggcg tcgacgaaac tcgtccctga tcacgcgctt cattcggttg
     4021 cgttcgtggg ccctgccgac ggcccgcttg ccgactatca tccccaggcg ggcgccctgc
     4081 attgtattcg ctaccgccgt cagccgtaac ggcccgctcc ggcggcgcac ctcggcgttc
     4141 ttcaggacgc ggtcgaagtc ccgcgatctg gttagccggc gccttcgcgg aaatgagtgg
     4201 atcgaagcag ctgcgcgatc gcctgcctca gacggagagg cgtttgcgac ctttggcgcg
     4261 cctcgcgttg atgacctggc ggccacctcg agtggacata cgcgaacgga agccgtgagt
     4321 gcgcttgcgc tttagattgc ttggctgaaa cgttctcttc atgttcgggt ttccgttgct
     4381 gacttaccga agctcccgcc cctttggcgc gctttagtcg gcggccggga tcgcgaggga
     4441 gcgggatttt atggatcagg ccccttgagt gtcaacgcag ctgcgacatc actccttctc
     4501 atagtaagaa atatatatag a
//
LOCUS       DBNHQR010000360         1998 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1301898, whole genome shotgun sequence.
ACCESSION   DBNHQR010000360 DBNHQR010000000
VERSION     DBNHQR010000360.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1301898"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            278..1243
                     /locus_tag="AC16VR_08055"
     CDS             278..1243
                     /locus_tag="AC16VR_08055"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6011149.1"
                     /translation="MGLGIRPFDKGLLDLGNGAYAWLQPDGSWGWSNAGLVVDGDASL
                     LVDTLFDKHLTREMLSAMRDAAPKATRQFDTLVNTHSNGDHCNGNELVTGAEIVASKA
                     CAEELAAENPRMMQTMMERAPEMGEVGEFFVHCFGAFDFTNINHTLPTRTFEGELGLT
                     VGDKAVLLKQVGPAHTRGDVLAYVPDEKLIYTGDILFIEGHPILWVGPVGNWIEACDY
                     MLGLDLENVVPGHGPVTDKRGVMAVRDYLIYIRDEARKRFDAGMPPDEAAVDISLTDY
                     DSWGDAERIVVNVAVLYKEFAADPEPGDIAELFALMAKVHKARRR"
     gene            1240..1731
                     /locus_tag="AC16VR_08060"
     CDS             1240..1731
                     /locus_tag="AC16VR_08060"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6011150.1"
                     /translation="MTDLRRAGEDDLPALVRIYNHYVAHSHCTFDIETYSVDERRGWW
                     RRFDAARYQCWIAEHAGRVIGYACSQPLKAKGGYETSVEVSVYVDAAHTGSGTGRSLY
                     QQLLAALAPEDVHRAYALVALPNDPSIALHQTMGFREVAHLGEVGRKFGRYWDVIWLE
                     RAL"
     gene            complement(1728..>1998)
                     /locus_tag="AC16VR_08065"
     CDS             complement(1728..>1998)
                     /locus_tag="AC16VR_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011151.1"
                     /translation="QPLPVAAIRATLLCGFAMTTSVRTVLVGVNGGLTGEVWLLTGLA
                     LPVVVLGTWAGRTFEPPVSELAMKRLAFAVLLVMGIWILLRAALG"
BASE COUNT          373 a          678 c          638 g          309 t
ORIGIN      
        1 gtcttgcgtc tgaggtaggc tctgaccagg acgctcgcca ggcggggcca gacccgggcc
       61 aacccgggca tgaggtagac ggccaccgcg cggtcgagtt ctgcgtcttc gaccaggcgc
      121 gcgaccaccc ccgcaacctc cgggtcctcg tagggtcgaa tggcttcgaa gtccagcatg
      181 tgcgccagtt agcgccattt gacaggcaga aaacaagtcg ccgcccgggc ggcttttcct
      241 ctaacatgag gagcctgctc ggtgggggag acatctgatg ggcctaggca ttcgcccctt
      301 tgacaagggc ctgctcgacc tgggaaacgg cgcatacgcc tggctccagc ccgacggcag
      361 ctggggttgg tcaaacgccg gcctggtggt cgacggggac gcctccctgc tggtggacac
      421 gctcttcgac aagcacctga cccgcgagat gctcagcgcc atgcgcgacg ccgcacccaa
      481 ggccacccgg caattcgaca ctctggtcaa cacgcactcc aacggcgacc actgcaacgg
      541 taacgagctg gtgaccgggg ccgagatcgt cgcctcgaaa gcctgcgcgg aagagctcgc
      601 cgccgagaat ccaagaatga tgcagacgat gatggagcgc gcgccggaaa tgggcgaagt
      661 gggcgagttc ttcgttcact gtttcggcgc tttcgacttc accaacatca accacactct
      721 gcccacccgt accttcgagg gagagcttgg actgaccgtc ggtgacaagg cggtgctgct
      781 caagcaggtc gggcccgccc acacccgagg cgacgttctg gcctacgttc ccgatgagaa
      841 gctcatctac acaggcgaca tcctcttcat cgaaggccac ccgattctct gggtgggccc
      901 cgtgggcaac tggatcgaag cctgcgacta catgctcggt ctggacctgg agaacgtggt
      961 gcccggacac ggtcccgtca cggacaaacg cggcgtgatg gccgtgcgcg actacctgat
     1021 ctacattcgt gacgaggctc gcaaacgatt cgatgccggc atgccgccgg acgaggcggc
     1081 tgtggacatc tcgctgacgg actacgactc ctggggcgac gccgagcgga tcgtggtcaa
     1141 cgtggccgtg ctctacaagg agttcgctgc agatcccgag cccggcgaca tcgccgagct
     1201 gttcgcgctg atggccaagg tgcacaaggc ccggcgccgt tgaccgacct gcgccgcgcc
     1261 ggggaggacg atctgcctgc cctggtccgg atctacaacc actacgtggc ccactcccac
     1321 tgcacgttcg acatcgaaac ctacagcgtc gacgagcggc gagggtggtg gcggcggttc
     1381 gacgccgccc gataccagtg ctggatcgcc gagcacgccg gcagggtcat cggctatgcc
     1441 tgcagccagc cgctgaaagc caagggcggt tacgaaacct cggttgaggt gagcgtctac
     1501 gtggacgccg cccatacggg ctcaggaacc ggacgctccc tctaccagca gctgctcgca
     1561 gcgctcgcgc ccgaggacgt gcacagggcc tacgccctgg tcgccctgcc caacgatccc
     1621 tccatcgctt tgcatcaaac gatgggcttc cgggaggtgg cgcacctcgg cgaggtcggc
     1681 cggaagttcg gccgctactg ggacgtgatc tggctggaac gcgcccttta gccgagcgcg
     1741 gcgcgcagca ggatccagat acccatgacg aggagcacgg cgaacgccag ccgcttcatg
     1801 gcgagctcag ataccggggg ctcgaaggtg cggcccgccc aggttcccag caccaccacg
     1861 ggcaaagcca gcccggtgag cagccacacc tcccccgtca acccgccatt gacccccacc
     1921 agcaccgtgc ggaccgacgt cgtcatggcg aagccgcaca gcagcgtggc ccgaatggcc
     1981 gccacgggca gcggttgc
//
LOCUS       DBNHQR010000361         2583 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_541280, whole genome shotgun sequence.
ACCESSION   DBNHQR010000361 DBNHQR010000000
VERSION     DBNHQR010000361.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_541280"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="AC16VR_08070"
     CDS             <1..639
                     /locus_tag="AC16VR_08070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR6011152.1"
                     /translation="LDVPRDAIVWEKGEARAKGTQYSNLPKLSLREIAAKAARTGGPI
                     AGHNEVTADGAGVAFAAHICDVEVDPETGLTRVLRYTVIQDAGKAIHPDYVEGQFQGA
                     AAQGIGWGLNEEYVYGEDGRLQNPGFLDYRIPVCSDLPFIDTKILEVPNPNHPYGIRG
                     VGEASIVPPLGAIGNAIANATGVRMQHVPMSPVRVLDALDQAEASESQVSAG"
     gene            629..871
                     /locus_tag="AC16VR_08075"
     CDS             629..871
                     /locus_tag="AC16VR_08075"
                     /inference="COORDINATES: protein motif:HMM:NF014636.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR6011153.1"
                     /translation="MLVSLNGALRAAAGGQDSVNVEASTIRQLIERLVDRYPAMADEV
                     DKGIAVAIDGVVYRDDWTQRIPENAEVFLMPRIAGG"
     gene            900..1724
                     /locus_tag="AC16VR_08080"
     CDS             900..1724
                     /locus_tag="AC16VR_08080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014664.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR6011154.1"
                     /translation="MADWDERKRRARESYQRFVPDADPERVLASMERSLGALGTFAFA
                     AVGEMWDRPELSRRDRSLMVIAVLAAQARDEELTLHTRIGMRHGLSRTEIEEINLHVA
                     AYAGFPAAMAAQRRMDQAFREAQGVEHIDDREGAEHLGDDERLRRAADVRRTLAGAEA
                     ALDPEADLASLRDYLGGVGEWAMKWAFGEVWSRPQLSRRDRSLVVVAILTALGQEAEL
                     AFHIPWGLNHGLTRAEVEEIMTHLCLYTGFPRAVEGMRAAKHAFAELDGQATAQPQ"
     gene            1757..>2583
                     /locus_tag="AC16VR_08085"
     CDS             1757..>2583
                     /locus_tag="AC16VR_08085"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6011155.1"
                     /translation="MIDVDAHLHEPLDWIEQTDPALARELGPPARFMDVANSVFGIAN
                     PALAELSEGQPPSGGYETILPGFVHHLEMTDQRQPERQCGSDDDPFCGAEARLAFCDE
                     RGIDVQFLNPTFLVGAFVQAARARRFELMGEIRQCWNRWAADQVHGHADRLIPVTQID
                     LSNVPRSIEEMTRMRERGSRAFAIPESPVGKPRFARNGQPALAKSLTHPDFEPLWSAA
                     EDLGMAAIAHVGFSRERINAGWANNVADDLMTYSFLNMVVNPQLAPQLLLAALAFDGV
                     "
BASE COUNT          454 a          848 c          868 g          413 t
ORIGIN      
        1 ctggacgtgc cccgggacgc catcgtctgg gagaagggcg aggcgcgtgc aaagggtacc
       61 caatacagca acctgcccaa gctctctctc agggagatcg ccgccaaggc cgcacgcacg
      121 ggtggcccca tcgcgggcca caacgaggtg acggcggacg gggcgggtgt cgctttcgcc
      181 gctcacatct gcgacgtcga ggtggatccc gagaccggac tgacccgtgt gctgcggtac
      241 acggtgattc aggacgcggg caaggccatt catccggact acgtggaagg ccagttccag
      301 ggcgccgcgg cccagggcat tggctggggg ctaaacgagg aatacgtcta cggcgaagat
      361 ggtcgcctgc agaatccggg cttcctggac taccggatcc ccgtctgctc agacctgccc
      421 ttcatcgaca cgaagattct ggaagtcccg aaccccaacc atccctacgg tatccgtggg
      481 gtgggtgaag cgtccatcgt tccaccgctg ggcgccatcg ggaacgcgat cgccaacgca
      541 accggtgtgc gcatgcagca cgtgccgatg tcgccggtac gcgtgctcga cgccctggat
      601 caggcagagg cgtcggaaag ccaggtcagt gctggttagc ctcaacgggg cgctgcgtgc
      661 tgccgccggg ggccaggact cggtgaacgt cgaggcgtca accattcggc agctgatcga
      721 gaggttggtg gatcgctatc cggccatggc cgacgaggtg gacaaaggca tcgccgtcgc
      781 catcgacggc gtggtctacc gtgacgactg gacccagcgg attccggaga acgccgaggt
      841 ctttctcatg ccgcgcattg cgggtggttg agccagaaaa aaccgagcag aggtaagcca
      901 tggctgactg ggacgagcgc aaacggcgcg cgagggaatc gtaccaacgc ttcgtaccgg
      961 acgcagaccc cgagcgcgtg ctggcatcca tggagcgcag cctcggcgcg ctgggaacgt
     1021 ttgcttttgc tgcggtcggc gagatgtggg accggcccga gctcagccgc cgggatcgca
     1081 gcctcatggt catcgccgtg ctggcggccc aggcccgcga cgaggagctg actctgcata
     1141 cccgcatcgg gatgcgacac gggctcagcc gcaccgagat cgaggagatc aacctccacg
     1201 tcgccgccta cgcgggcttt cctgcggcca tggccgcaca gcggcgtatg gatcaggctt
     1261 ttcgcgaagc ccaaggcgtg gaacacatcg acgaccgcga gggagcggag catctcggcg
     1321 acgacgagcg gctgcgtcgg gcggccgacg tgcggcgcac gctcgccggc gccgaggctg
     1381 ccctcgatcc ggaagccgat ctggcgtcgc tgcgcgacta tctcggcggc gttggcgagt
     1441 gggcgatgaa atgggcgttc ggggaggtct ggtcgcggcc gcagctctcc cgcagagacc
     1501 gctccctggt ggtcgtcgcc atcctgaccg ccctcggcca ggaggccgag ctcgcctttc
     1561 atatcccctg gggtctaaac catggcctta cgcgcgctga ggtcgaggag atcatgaccc
     1621 acctgtgcct gtacaccggg tttccaaggg cagtcgaggg catgcgggcc gccaagcacg
     1681 ccttcgcgga gctcgacggg caggccacgg cacaaccgca atgactcgtg actctggggg
     1741 gattcacgcg atccgcgtga tcgacgtcga cgcccatctt cacgagccgc tcgactggat
     1801 cgagcagaca gaccccgcgc tcgccaggga gctggggccg ccggcacgct tcatggacgt
     1861 ggctaattcg gttttcggga tcgcgaatcc cgctctcgcc gagctgtccg aggggcagcc
     1921 cccctcgggt ggctacgaaa ccatcctgcc tggcttcgtg catcacctgg agatgaccga
     1981 ccagcgccaa ccggagcgtc agtgtggatc agacgacgac cctttctgcg gagccgaggc
     2041 gcgcctggcg ttctgcgacg aacgaggcat cgacgtccag ttcctgaacc ccacgttcct
     2101 ggtgggagcg ttcgtccagg cagcacgcgc acgacgcttc gagctcatgg gcgaaattcg
     2161 ccagtgctgg aaccgatggg cggccgatca ggtgcacggc cacgcggacc ggctcattcc
     2221 ggtcacacag atcgacctgt ccaacgtccc gcggtccatc gaggagatga cgcgcatgcg
     2281 tgagcgcggc agccgggcgt tcgcgatccc ggagtccccg gtgggcaaac cgcgattcgc
     2341 acgcaacggc cagcccgcgc tggccaaatc tctgacccac ccggatttcg aaccgctgtg
     2401 gtcggcagcc gaggacctgg gcatggcggc catcgcccac gtcggctttt cccgggaacg
     2461 catcaacgcc ggctgggcca acaacgttgc cgacgatctg atgacctaca gctttctgaa
     2521 catggtggtc aacccccagc tcgctccgca gctgctgctg gccgctctgg ccttcgatgg
     2581 cgt
//
LOCUS       DBNHQR010000362         7679 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1362927, whole genome shotgun sequence.
ACCESSION   DBNHQR010000362 DBNHQR010000000
VERSION     DBNHQR010000362.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7679)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7679)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7679
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1362927"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="AC16VR_08090"
     CDS             <1..375
                     /locus_tag="AC16VR_08090"
                     /inference="COORDINATES: protein motif:HMM:NF025670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HIR6011156.1"
                     /translation="SQTGYFKASNAELGDEFGSAVAYEGDTLVVGARKEAGLSGNEND
                     NSGARVGAAYVFFREDGIWRQAAYVKASNPDNEDEFGNAVSFDGNALVVTAEGEDSAE
                     TGLGGAGLDNSLNAAGAGYLFR"
     gene            complement(364..1950)
                     /locus_tag="AC16VR_08095"
     CDS             complement(364..1950)
                     /locus_tag="AC16VR_08095"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HIR6011157.1"
                     /translation="MVSGLHPLHWAEVEPDKTAWIFGDQRVSYARLVQSALKGAQLLR
                     KLGLKTGDGVAVVAENHPNTLAFFWAAQMAGLYYTPISVQFHPDEIGYILADCDARLL
                     MASRAQEERVAGAPQPMRLTLDTWLDAIAGEPATLIADACEGAEMLYSSGTTGRPKGV
                     RAASPGAPLGTVSELFRRRLALHEVDGQAVYLSTAPLYHSAPLRYNTMMHRCGATSVI
                     MPRFDAAESLALIETHRVTHSQWVPTMFVRLLKLPAETREAFDLSSHRLAVHAAAPCP
                     VPIKRQMLDWWGPILHEYYSGTEGNGQTAITPEEWLAHPGSVGKPVLGNVHILDEAGN
                     EAPIGTEGCVYFGDGARFEYYKDPAKTAASYDERGWSTLGDIGYLDQEGYLYLTDRAS
                     HMIITGGVNVYPREVEDVLVTHPAVQDAAVFGIPDPEFGEQVKAAIELVPGTTVNGDE
                     LIAFCRARIAHLKCPKSVDFHDTLPRHQTGKLYKEALKRPYWPEPSAKKEPSANKEPS
                     ANKEPSANKEPSANKEPSAE"
     gene            complement(1944..2279)
                     /locus_tag="AC16VR_08100"
     CDS             complement(1944..2279)
                     /locus_tag="AC16VR_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003249769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1820 family protein"
                     /protein_id="HIR6011158.1"
                     /translation="MSDDQKHIYKVLFHNQGQIYEVYAHNIYQSDLYGFIEVEDYIFG
                     KKTQVVIDPAEDKLRTEFDGVQRSFIPMHAVIRIDEVEREGVAKVTESSGGNITPFPV
                     PMPKDTGKS"
     gene            2372..3703
                     /gene="miaB"
                     /locus_tag="AC16VR_08105"
     CDS             2372..3703
                     /gene="miaB"
                     /locus_tag="AC16VR_08105"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010222916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HIR6011159.1"
                     /translation="MMKKLYIKTHGCQMNEYDSARMADLLRESQGYELVAEPEDADLI
                     LLNTCSIREKAQEKVFHQLGRWRALKVRKPGLVIGVGGCVASQEGAAIQSRAPHVDVV
                     FGPQTLHRLPEMIDAAAGPARVPLVDVSFPEIEKFDRLPQPRAEGPSAFVSVMEGCSK
                     YCSFCVVPYTRGEEVSRPVADVMREVIQLAGQGVREVNLLGQNVNSYLGPIDDDHADL
                     AELIYYVAEVDGIDRIRFTTSHPLDFNDSLVRAFADVPELVDHLHLPVQSGSDRVLAL
                     MKRGHTVSEYREKIAALKEVRGGISLSSDFIVGFPGETEADFQATMDLVAEIGFDTSF
                     SFVYSARPGTPAAALPDDTPEAVKRERLALLQGLLRSQADAIACDMVGTRQTVLVTGE
                     SRKNREELQARTENNRVVNFPCPDRRLVGGFVDVDITEALPNSLRGVMAIT"
     gene            3796..4833
                     /locus_tag="AC16VR_08110"
     CDS             3796..4833
                     /locus_tag="AC16VR_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIR6011160.1"
                     /translation="MSEDDSRVVALTTNVSLEPNEPRRLAALCGPFDQNLKHLERRLG
                     VHIRSRGNLFQISGPEQRVQAAGALLSQLYRETLEHETIDPDMVHLFLQESGVDELLA
                     RQRGPDEMPADGEGAVISTGRKSVKPRGGNQTRYVERIRGHDVNFGIGPAGTGKTYLA
                     VACAVQALESQQVSRILLVRPAVEAGEKLGFLPGDLAQKIDPYLRPLYDALYEMMGVD
                     RVNKAIERNIIEVAPLAYMRGRTLNNAFIILDESQNTTIAQMKMFLTRIGFGSTAVIT
                     GDITQIDLPKGQKSGLVNVRNVLSGIDGISFTHFGSKDVVRHPLDQKIVDAYEAYQEP
                     HASPDSDRDAG"
     gene            4823..5278
                     /gene="ybeY"
                     /locus_tag="AC16VR_08115"
     CDS             4823..5278
                     /gene="ybeY"
                     /locus_tag="AC16VR_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017903146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HIR6011161.1"
                     /translation="MPVEVQIASVQAGLPSVARIEGWASAALDELGDEGEPPELCIRV
                     VDGEESRELNARFRGHDKPTNVLSFPADVDLPEAKLLGDVVICAPVVGVEAAEQGKSP
                     EDHFAHMVVHGVLHLSGYDHEQAGEAELMEAIERRILERVGIADPYAER"
     gene            5285..6120
                     /locus_tag="AC16VR_08120"
                     /pseudo
     CDS             5285..6120
                     /locus_tag="AC16VR_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020798742.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
     gene            6117..7598
                     /gene="lnt"
                     /locus_tag="AC16VR_08125"
     CDS             6117..7598
                     /gene="lnt"
                     /locus_tag="AC16VR_08125"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HIR6011162.1"
                     /translation="MKPLLALACGALVPLSLAPFGWWPLGLVAVGGWFALLQSSDRGG
                     LLLGWLFGLGKYAAGVSWIYVSINVYGNAPPPLAAFLVALFAGGLALFPMVNAWVFQR
                     LRTKGAPLVNAWLFACLFVAFEWLLTWFLTGFPWLYPAYGHVETVLGGLAPAGGVMLV
                     SLGLAGSACSVAAAHQCARRLPVLTAALAPWVVGAVLGAVSWVEPGAVRTVALVQGNV
                     DQAVKWLPENRRPIIDRYRGLSEDHWGVDVMVWPEAAITVMAHDAGEVLGDLEREGER
                     SGTAVVLGLPAVERLADDRIVLRNTAIALGDGSGRYVKRRLVPFGEYVPLEGWLRGLI
                     EFFDLPMSRAAPGDWRQPLLRVGQEQAALAICYEIVYPDLVRAQAVGADVLVTISNDT
                     WFGASIGPLQHLQMARMRALENGRWLLRGTNNGVTAIVDHRGRLVARLPQFEAGVLRG
                     EYRLVAGRTPYNRHGDGPVLVLVAFGLGVAFWRRLGAARGADG"
BASE COUNT         1268 a         2378 c         2614 g         1419 t
ORIGIN      
        1 agccagacgg gctacttcaa ggcgtccaac gcggagcttg gcgacgagtt cggctcggcc
       61 gtggcctacg agggcgacac cctggtggtg ggcgcccgca aggaggccgg tttgagcggc
      121 aacgagaacg acaattcggg ggcgcgtgtg ggggccgcct acgttttctt ccgtgaggac
      181 ggcatctggc gccaggccgc gtacgtgaaa gcgtccaacc ctgataacga ggacgagttc
      241 ggcaatgctg tgagctttga cggcaacgct ctggtggtca ccgcggaagg cgaggacagc
      301 gcggagaccg ggctcggcgg agcgggtctc gacaactccc tgaacgccgc gggcgccggg
      361 tacctattcc gctgaaggct ctttgttcgc tgaaggctct ttattcgctg aaggctcttt
      421 attcgctgaa ggctctttat tcgctgaagg ctctttcttc gctgaaggct ccggccagta
      481 gggccgcttg agggcttctt tgtagagctt gcccgtctgg tggcgcggca gggtgtcatg
      541 gaagtccacc gacttcgggc acttcaggtg ggcgatgcgc gcccggcaga aggcgatgag
      601 ctcgtcgccg ttcactgtgg tgccggggac gagctcgatg gcggctttga cctgctcgcc
      661 gaattccggg tccggaatgc cgaacacggc cgcgtcctgc accgccggat gggtcaccag
      721 cacgtcctcc acctccctcg gatagacgtt taccccgccg gttatgatca tgtgcgaggc
      781 gcgatcggtg aggtacaggt agccttcctg gtccagatag ccgatgtcac ccagagtgga
      841 ccatccgcgc tcgtcgtagg aggccgccgt cttcgccgga tccttgtaat actcgaaacg
      901 cgctccgtcg ccgaagtaaa cgcatccctc ggtgccgatc ggcgcttcgt tgccagcctc
      961 gtccaggatg tgcacgttgc cgaggacggg cttgcccacg gaccccgggt gggcgagcca
     1021 ctcttcgggc gtgatggccg tctgcccgtt gccctccgtg cccgagtagt actcgtggag
     1081 gatggggccc caccagtcga gcatctgccg cttgatgggc acggggcacg gtgccgcggc
     1141 atggacggcc aggcggtggc tggaaaggtc gaacgcttcc cgggtctctg ccggcagctt
     1201 cagcagccgt acgaacatgg tgggcaccca ctggctgtgg gtgacccggt gagtctcgat
     1261 cagcgccagg ctttcggcag cgtcgaagcg cggcatgatc acgctggtgg cgccgcagcg
     1321 gtgcatcatg gtgttgtagc gcagcggcgc ggagtgatac agcggcgcgg tggagaggta
     1381 gacggcctgg ccgtccacct cgtgcagcgc cagccggcgc ctgaacagct cggagaccgt
     1441 gccgagaggg gcgcccggac ttgccgcgcg cacgcctttg ggccggcccg tggtgccgga
     1501 ggagtagagc atctccgcgc cctcgcaggc gtcggcgatc agggtcgcgg gctcgccggc
     1561 aatggcgtcg agccaggtgt ccagagtcag gcgcatcggc tggggcgcgc cggccacccg
     1621 ctcctcctgg gcgcggctcg ccatcagcag ccgggcgtcg cagtcagcga ggatgtagcc
     1681 gatctcgtcg ggatgaaact ggacgctgat gggggtgtag tagagccccg ccatctgtgc
     1741 cgcccagaag aatgccaggg tgttcgggtg attctccgcg accaccgcga cgccgtcgcc
     1801 cgttttcagg ccgagcttgc gcaggagctg cgcgcccttc agcgcgcttt ggaccagccg
     1861 cgcgtagctc acgcgctgat cgccgaagat ccatgcggtc ttgtcgggct cgacctccgc
     1921 ccagtggagc gggtgaaggc cgcttacgat ttgccggtgt ccttgggcat gggaaccggg
     1981 aagggggtga tgttgccgcc gctggactcc gtgactttcg ccacgccctc gcgttcgacc
     2041 tcgtcgatgc ggatcacggc atgcatgggg atgaagctgc gctgcacgcc gtcgaactcc
     2101 gtgcgcagct tgtcctcggc cgggtcgatg accacctggg tcttcttgcc gaagatgtag
     2161 tcctccacct cgatgaagcc gtagaggtcg ctctgataga tgttgtgcgc gtagacctcg
     2221 tagatctgac cctgattgtg aaacagcact ttgtagatgt gcttctggtc gtcgctcatt
     2281 ggcgttgata cctgctgtgc cagatgtcct atatagaggt gtatgtggaa aattcaagca
     2341 tttataatcc cgcgccccga atccgggtcg tttgatgaag aagctctaca taaaaaccca
     2401 cggttgccag atgaacgagt acgactcggc ccgcatggcc gatctgctgc gggagagcca
     2461 gggttacgag ctcgtcgccg agccggaaga cgcggacctg atcctgctga acacctgctc
     2521 gatccgcgag aaagcccagg agaaagtctt tcaccagctg ggtcggtggc gcgcgctcaa
     2581 ggtgcgcaag cccgggctgg tgataggcgt cggggggtgc gttgccagcc aggaaggcgc
     2641 ggccatccag agccgggcgc cccacgtgga cgtggtattc gggccgcaga ccctgcaccg
     2701 gctgcctgaa atgatcgatg ccgccgcggg tcccgcacgg gttccgctcg tggatgtgag
     2761 ctttccggag atcgagaagt tcgaccgtct gccccagccg cgggctgaag ggccctcggc
     2821 cttcgtctcc gtgatggagg gctgcagcaa gtactgcagc ttctgcgtgg tgccgtacac
     2881 ccgcggagag gaggtcagcc gaccggttgc cgacgtgatg cgcgaagtga tccagctcgc
     2941 cggtcaagga gtccgggagg tgaacctgct cgggcagaac gtgaattcct acctgggccc
     3001 catcgacgac gaccatgcag acctggcgga gttgatctac tacgtggccg aagtcgacgg
     3061 catcgaccgc atccgcttca ccacctctca tccgctggac ttcaacgatt cgctcgttcg
     3121 cgcgttcgcc gacgttccgg aactggtgga tcatctgcat ctgcccgttc agtccgggtc
     3181 cgaccgggtg cttgctctga tgaagcgggg ccacaccgtg agcgagtatc gtgaaaagat
     3241 cgcggccctc aaagaggtgc gagggggcat cagcctttcg tcggacttca tcgtcggctt
     3301 ccctggcgag accgaggcgg acttccaggc caccatggac ctcgtcgccg agatcgggtt
     3361 cgatacctcg ttcagcttcg tctacagcgc acgccccggg actccggcgg ccgccctgcc
     3421 ggacgacacg ccggaagccg tcaagcgcga gcgcctggcg ctcctgcagg gcctgctgcg
     3481 ctctcaggcg gatgccatcg cctgcgacat ggtcggaacg cggcagacgg tgctggtcac
     3541 gggcgagtcc aggaagaatc gggaagagct gcaggcgcgc accgagaaca atcgggtcgt
     3601 caacttcccc tgtccggacc gtcgcctcgt tggcggcttc gtggacgtgg acattaccga
     3661 agccctgcca aactccctgc ggggcgtgat ggcgatcaca tagggcgcag acgggcgttt
     3721 ggggcggcga aacccgattt gacaggtaac tgcgcgccga tatcctagac aaccctcttc
     3781 tgcacagggc gcgcattgag cgaagacgat tcacgggtgg tggcgctcac cacgaatgtc
     3841 agcctggaac ccaacgaacc ccgccgattg gcagccttgt gcgggccgtt cgaccagaac
     3901 ctcaaacacc tcgagaggcg gctgggcgta catattcgca gccgcggcaa cctattccag
     3961 atcagcgggc cggagcagcg tgttcaggcg gccggcgcgc tgctgtccca gctataccgg
     4021 gagacgctcg agcacgagac catcgacccg gacatggttc acctgttcct tcaggaatcg
     4081 ggcgtcgacg agctgctcgc gcgtcagcgc gggccagacg agatgcccgc ggacggggag
     4141 ggtgcggtca tctcgaccgg gcgaaaatca gtcaagcccc ggggcggcaa ccagacccgt
     4201 tacgtcgagc ggattcgcgg ccacgatgtg aatttcggca tcgggccggc cggaaccggc
     4261 aagacttacc tggcggtggc ctgtgctgtg caggcgttgg aaagccagca ggtgagccgg
     4321 attctgctgg tgcgcccggc cgtggaggcg ggcgagaagc tgggctttct gcccggggat
     4381 ctggcccaga aaatcgaccc ctacctgcgc ccgctctacg acgccctgta cgagatgatg
     4441 ggcgtggatc gcgtcaacaa ggccatcgag cgcaacatca tcgaggtggc cccccttgcc
     4501 tacatgcggg gccggacgtt gaacaatgcc ttcatcatcc tcgacgagag ccagaacacc
     4561 accatcgccc agatgaagat gtttctcacg cgaatcgggt tcggctccac tgcggtgatc
     4621 accggcgaca tcacccagat cgacctgccc aaagggcaga agtcggggct ggtcaacgtg
     4681 cgcaacgtgc tcagcggcat cgacggcatc agcttcaccc acttcggctc caaggacgtg
     4741 gttcgccacc ccctggatca gaagatcgtc gacgcttacg aggcatatca ggagcctcat
     4801 gcaagcccgg acagcgaccg tgatgccggt tgaagtgcag atcgcatccg tccaggcggg
     4861 gcttccgtcc gtcgcgcgca tcgagggctg ggcgtccgcc gcgctggacg agctcgggga
     4921 cgaaggcgag ccgccggagc tgtgtatccg ggtcgtcgac ggagaggagt cgcgggagct
     4981 caatgcccgt ttccgcggtc atgacaaacc aaccaatgtg ctgagctttc cggccgacgt
     5041 cgatctgccg gaagccaagc tgctcggaga cgtcgtcatc tgcgcgcccg tggtaggcgt
     5101 cgaggcggcc gaacagggca agtcgccgga ggatcacttc gcgcacatgg tcgtgcacgg
     5161 ggtgcttcat ctgagcggct acgatcatga gcaggcgggg gaagcggagc tcatggaagc
     5221 gatcgaaaga cgaatactgg agcgggtcgg catagccgac ccctacgccg agcgatgagc
     5281 gaggtcggag gatcggagca gcagcggcgt tccttgctgg actggctcgc agaccttttc
     5341 aacgacgagc ccacggaccg ttccgagctg atggagacgc tgcgcgatgc tgccgaccgg
     5401 cagatcatgg atagcgaggc gctcaacatc attttcgggg ccctgcaggt gtcggacatg
     5461 caggcgcggg acatcatgat tccgcggacc cagctcgtgt atctggagga ctccaaggcc
     5521 cccgaggagc tgctcgccac catcatcgag tctcagcatt cgcgctttcc ggttgtcggc
     5581 gatgatctgg acgacgtcaa aggcattctg cacgccaagg atctgctgcc gctggcgctc
     5641 tccagcggct cgggcagcaa tggcttcgac atcaaggact gcatccggcc ggcaacgatc
     5701 gttccggaaa gcaagcggtt gaacgtcctg ctgcaggagt tcagaagcac ccgcaaccat
     5761 atggcggtcg tggtggacga gtacggtcac gtttccggcg tggtgaccat cgaggacgtg
     5821 ctggagcaga tcgtgggcga gatcgaggac gagcacgacg tcatcgacga cagcttcgtc
     5881 aagcagctcg attcccgaac gttcaacgtg aaggcaacga ctaccatcga ggatttcaac
     5941 gagcacttcg acctcgagct cgacgacgac gagttcgaca ccattggcgg cctcgtgctc
     6001 aaggcgttcg gccacctgcc cgatcgcgga gagtcggtgc ggatcggaga tctcgacttc
     6061 aaagtgctca atgccgattc gcgaagagtc aggctgctgc aggtcaagct gcgcaaatga
     6121 agcccctcct cgcgctcgcc tgcggcgcgc tggtccctct ctctcttgca cccttcggct
     6181 ggtggccgct tgggctcgtc gccgtaggtg gctggtttgc tttgctgcag tcgagtgatc
     6241 ggggcgggct gcttctgggc tggctgtttg gtttaggcaa gtacgcggcc ggcgtatcct
     6301 ggatctacgt cagcatcaac gtttacggca acgcgccgcc gccgctggcc gccttcctcg
     6361 tcgctctgtt tgccggtggg ctcgcgctgt tccccatggt aaatgcctgg gtgttccagc
     6421 gcctgcggac caagggcgcg ccgctcgtca acgcctggct gttcgcctgc ctgttcgtgg
     6481 cgttcgagtg gctgctcacc tggtttctga ccggcttccc ctggctctat cccgcctatg
     6541 gtcacgtcga gacggtcctc ggcgggttgg cccccgcggg cggggtgatg ctggtgagcc
     6601 tcgggcttgc cgggtcggcc tgctccgtcg ccgcggccca tcagtgcgcg cgacggcttc
     6661 ccgtgctcac ggcggctctg gcgccctggg ttgtcggtgc cgttctgggc gcggtttcct
     6721 gggtcgagcc cggggccgtt cgcaccgtag cgctcgtgca gggcaacgtg gatcaggccg
     6781 tgaagtggct gcccgagaac cggcggccca tcatcgatcg ctaccggggg ctgtcggagg
     6841 accattgggg cgtggacgtc atggtctggc cggaagccgc catcacggtg atggcccatg
     6901 acgccgggga ggttctgggt gatctggagc gcgagggcga gcggtcgggc accgctgtcg
     6961 tgctggggct gccggcggtg gagcggcttg ccgacgatcg gatcgtcctg cgcaacacgg
     7021 ccattgcctt aggggatgga tcggggcgtt acgtcaagcg gcggctcgtt cccttcggcg
     7081 agtacgtccc gttggagggg tggctgcggg ggctcatcga gttcttcgat ctgcccatgt
     7141 cgcgcgctgc gcccggcgat tggcgacagc ctctgctcag ggtcggtcag gagcaggcgg
     7201 cgctggccat ctgctacgag atcgtctatc cggatctggt cagggcccag gccgtcgggg
     7261 cagacgtgct ggtcaccatt tccaacgata cctggttcgg tgccagcatc ggcccgctgc
     7321 agcatctgca gatggcgagg atgcgcgctc tggaaaacgg ccgctggctg cttcggggaa
     7381 ccaacaacgg ggtgaccgcc atcgtcgacc accggggcag attggttgcg cggctgcctc
     7441 agttcgaggc cggcgtgctg cggggcgagt accgactcgt ggcaggccga acgccctaca
     7501 accgtcatgg tgatgggccg gttctggtgc tggttgcctt cggtctcggc gtcgccttct
     7561 ggcgcaggct cggcgcggcg cgtggcgccg acgggtagtg tagtatgcgg cgcgtgcgca
     7621 cccgtctacg ctacacctgg ctgatcccgc ctctgttcct cctcatcggg ggctgcggc
//
LOCUS       DBNHQR010000363         2986 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_920541, whole genome shotgun sequence.
ACCESSION   DBNHQR010000363 DBNHQR010000000
VERSION     DBNHQR010000363.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_920541"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="AC16VR_08130"
     CDS             complement(<1..233)
                     /locus_tag="AC16VR_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011163.1"
                     /translation="MKRVLVFLLIGFSTPAFAGPRLYVFDCGLLSLDSVEIFNLAEDE
                     TSVRELFVPCYLIEHENGRMLWDGGLPLSYAQA"
     gene            complement(302..616)
                     /locus_tag="AC16VR_08135"
     CDS             complement(302..616)
                     /locus_tag="AC16VR_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011164.1"
                     /translation="MTIKSAIYDLLVSRRYDAELETVTEDARRRCVEMLAPRPGGRCA
                     MMDTYWPKPPLRLWLMSWSYAADPKRPGFELLRATCDDFSLEHFPPDVEHGFYLASGT
                     KR"
     gene            complement(629..1615)
                     /locus_tag="AC16VR_08140"
     CDS             complement(629..1615)
                     /locus_tag="AC16VR_08140"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR6011165.1"
                     /translation="MRVLVTGGNRYIGRELVFELARRGHELTVINSHESPLPEGTRRI
                     HADRRLPGEFEAALSAHRDDFDAVFDNTAFTLADLEPMVELFRGRVQHYVFTSSMAVY
                     KRSFVQPLKEDFRRHAADDDDPRKAYGVGKVRCEDLLLAEWQRDAFPATWLRVGHTMG
                     PRSPLPTRDPAFFARLEAGRPILVPGEGFAVLQLVHINDVARCMASLLGNERVRGEGY
                     NVSGSEVASVLGVVHLIGRAMGVQPRVVNVPMEVARAHRPPLLHWGESVSGSAMLSID
                     KALAHLDWEPRFGIEAGYRDAYEWWQREGRGLFEFDFAPEDALVERLGLATE"
     gene            1739..2287
                     /locus_tag="AC16VR_08145"
     CDS             1739..2287
                     /locus_tag="AC16VR_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003290281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6011166.1"
                     /translation="MTQTNGGLPGLRGVDHIGITAPDFDQAADFLENVLGLEAFYQMG
                     PFGSEETDWMLDMLNVHPRAVIRRVKQFRCGHGPNIELFEYESPDQRREMPKNSDWGG
                     HHITLYVDDIDAAMDHLRAHGVRLMAGGGPPVDDGPEAGLRSCYFLTPWDLQMELISY
                     PDGKAYERGTDRRLWDPRDPAR"
     gene            complement(2284..2673)
                     /locus_tag="AC16VR_08150"
     CDS             complement(2284..2673)
                     /locus_tag="AC16VR_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011167.1"
                     /translation="MTIFIRVLAALPALLFLVMGIQWLFAPAEAAAGLEMPLLQGVAA
                     STQIGDLGSFFFVNGVLMALGQLPGRSHLLYAPAFLIGGAGVFRIAAALLGHAPFAVD
                     FILAEAIMTTILLVAARRLGQPAAAAA"
     gene            complement(2698..>2986)
                     /locus_tag="AC16VR_08155"
     CDS             complement(2698..>2986)
                     /locus_tag="AC16VR_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011168.1"
                     /translation="TDTEPVLPEAATAPEGFPTRTELAARYAERTGRDLSKLDYYVGF
                     NRWKSAAIVHGVYARYCEGKKTADEGVDLDEMRGRIDRSLMLSEQAIARLR"
BASE COUNT          565 a          987 c          975 g          459 t
ORIGIN      
        1 tcggcctggg cgtaggacag cggcaagccg ccgtcccaga gcatacgccc gttctcatgc
       61 tcaatgaggt agcacggcac gaacagttcg cgtacggacg tttcgtcctc ggcgaggttg
      121 aagatctcca cgctgtcgag gctaagtaac ccgcagtcga agacgtagag ccgcggacca
      181 gcgaaagccg gcgtcgagaa accaatcagc agaaagacga gcactctctt cactttggga
      241 cgagtctcag gaacgggagc gacaaccata ccatcagagc agccggtgcc atgggccgga
      301 gtcagcgctt ggtgccactg gccaagtaaa agccgtgctc gacgtcgggc ggaaaatgct
      361 cgagagagaa atcgtcgcag gtcgcgcgga ggagctcgaa gcccggccgt ttcggatccg
      421 ccgcgtagct ccaggacatg agccagagtc tgagaggagg cttcggccag taggtgtcca
      481 tcatcgcgca acgcccgccg gggcgtggcg cgagcatctc cacgcagcgg cggcgcgcat
      541 cctcggtcac cgtctccagc tccgcgtcgt agcgccggga gacgagcaga tcgtagatgg
      601 ccgacttgat ggtcacgctg atcccgtctc actcagttgc caggccaagg cgctcgacca
      661 acgcgtcttc cggggcaaag tcgaactcga acagaccgcg gccttcccgt tgccaccact
      721 cgtaagcatc gcggtatccg gcctcgatgc cgaaccgggg ctcccagtcg aggtgagcga
      781 gggccttgtc gatggagagc atggccgatc cggacaccga ctcgccccag tgaaggagcg
      841 gcggacggtg agcgcgcgcc acctccatgg gcacgttgac gacacgcggc tgcacgccca
      901 tggcccgtcc gatcaggtgc acaacgccga gcacgctggc cacctcgctc ccggatacgt
      961 tgtagccctc acccctgacc cgctcgttgc ccagcaggga cgccatgcag cgcgccacgt
     1021 cgttgatgtg cacgagctgc aggacggcga acccctcgcc gggtacgagg atcgggcgtc
     1081 cggcttccag acgcgcaaag aacgcagggt ccctcgtggg cagcgggctg cgcggcccca
     1141 tggtatggcc gacgcgcagc caggtcgcgg ggaaggcgtc gcgctgccac tcggcgagca
     1201 gcaggtcctc gcacctgacc ttgccgaccc cgtaagcctt tcggggatcg tcgtcgtctg
     1261 ccgcgtgccg acggaagtcc tccttgagcg gctggacgaa gctgcgcttg tacacggcca
     1321 tggagctggt gaagacgtag tgttgaaccc gaccgcggaa gagctcgacc atgggctcga
     1381 gatcggccag cgtgaacgcg gtgttgtcga acacggcgtc aaaatcatcc ctatgggcag
     1441 acagagcggc ctcgaactcc ccgggcaacc ggcggtccgc atgaatgcgg cgggttcctt
     1501 ccggcagcgg ggactcgtgg ctgttgatga cggtcagctc gtgaccacgg cgggccagct
     1561 cgaagacgag ttcgcggcct atgtaacggt tgccgcccgt gaccagaact ctcatctagg
     1621 accctcccct gcgttcgggc tgaagatagc agaggccggc cacagcacgc catgcacacc
     1681 cccgggcgtt gctgctaacc ttggcggtgt cggcaacggg agcaaagggg agcatgtcat
     1741 gacccagaca aacggaggcc tgccgggact gcgcggcgtt gatcacatcg gcatcacggc
     1801 gccggacttc gatcaggcgg cggacttcct ggagaacgtt ctcggcctgg aggccttcta
     1861 ccagatgggc cccttcggct ccgaggagac cgactggatg ctcgacatgc tcaacgtcca
     1921 cccgcgggcc gtgatccgcc gggtaaagca gttccgctgt ggccacggac ccaacatcga
     1981 gcttttcgag tacgagtctc cggaccagcg gcgcgagatg ccgaagaaca gcgactgggg
     2041 cgggcatcac atcacgctgt acgtggacga catcgacgcg gccatggacc acctccgagc
     2101 ccacggcgtg cgcctgatgg ccggtggcgg gccgccggtg gacgacggcc ccgaggcggg
     2161 cctgcgttcc tgctacttcc tgaccccctg ggacctgcag atggagctga tctcctaccc
     2221 cgacggcaaa gcctacgagc ggggcaccga ccgtcgcctg tgggatcctc gggacccggc
     2281 ccgctaggcc gccgcagcgg ccggttggcc cagtcgtcgg gcggccacca gcagaatcgt
     2341 cgtcatgatc gcttcggcga gaatgaaatc caccgcgaag ggcgcatgac ccagcagggc
     2401 ggcggcaatg cgaaacacgc ccgccccgcc gatgagaaac gcgggggcgt agagcagatg
     2461 gctccggccg gggagctgac ctagagccat cagaaccccg ttgacgaaga aaaagctccc
     2521 aagatcgccg atctgcgtac ttgccgccac gccctgcagc agcggcattt caagacccgc
     2581 ggcagcttcc gccggcgcaa acagccactg aatgcccatg accaggaaca gcagcgcggg
     2641 caacgcggcg agcacccgga tgaatatcgt catgatcgtc tccctagcat tggccgttca
     2701 gcgcagccgc gcgatggcct gctctgaaag catgagcgac cggtcgattc gacctcgcat
     2761 ctcgtccaga tccaccccct cgtccgccgt cttcttcccc tcgcaatagc gcgcataaac
     2821 gccgtgcacg atggcggccg atttccagcg attgaagcca acgtagtagt cgagcttgga
     2881 gaggtcgcgt ccggtgcgct cggcgtagcg tgccgcaagc tcggtgcggg tcgggaatcc
     2941 ttcgggggcc gtggccgctt cgggcaacac gggttccgtg tcggtc
//
LOCUS       DBNHQR010000364         6289 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_920586, whole genome shotgun sequence.
ACCESSION   DBNHQR010000364 DBNHQR010000000
VERSION     DBNHQR010000364.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6289)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6289)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6289
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_920586"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="AC16VR_08160"
     CDS             complement(<1..678)
                     /locus_tag="AC16VR_08160"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR6011169.1"
                     /translation="MQSRPACFVAALLLAFVPAANAADWYPSRYGADDTLGAINNLSP
                     EKVVQAARLVTEGKTYPLGVETGPDSPAYPPRSYSLTVLQPGDGTGSLMGTNRASGND
                     DLLSFWMGIGSQIDGLGHMGIEHVYYNGNRAQDFVSATGLSRLSIDRLPPIVGRGVLI
                     DMAAHMGKAILPAGTAFNSREIKAAAKAQGVTIGTGDVVLFHTGWLNVADDKARVMAG
                     EPGLGVDG"
     gene            complement(723..2126)
                     /gene="mpl"
                     /locus_tag="AC16VR_08165"
     CDS             complement(723..2126)
                     /gene="mpl"
                     /locus_tag="AC16VR_08165"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020798772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HIR6011170.1"
                     /translation="MNAERRIYFLGIGGTLMGSLAQLAREMGYRVSGSDVAIYPPMSD
                     QLAAADIEVFEGFDPAHLDPEPELVVIGNAGLPRGHPGVEHIIENGLRYVSGAEWLGS
                     QVMPGRWVLAVTGTHGKTTTASMLAWILDQAGLKPGYLIGGVPKNFPRSSRLGDDPFF
                     VVEADEYDCSYFDRRSKFVHYHPRTLIINNLEYDHADIFPNLAAIQTQFNHLLRTVPG
                     HGLVIAPSNDDNVNETLQQGCWTPIARVGAPGVKQPLERDNGERWSASRGDGTGAEFD
                     VCLGEENLGTVRWPIFGDFNVRNALAAIAAARHAGVPPEVAIDALGSFEGVKRRLELI
                     AEIGATLIYDDFAHHPTAIRATLQGLRNKVGSEEIIAVIEPRSHTMSLGTLRNELATC
                     CSPADEVYWFRGENIQWDLSELLKSCVIPAHINFELDKLIEALAQLPQKRRHIVIMSN
                     GSFGGIYQKLPTRLRSG"
     gene            complement(2123..2623)
                     /locus_tag="AC16VR_08170"
     CDS             complement(2123..2623)
                     /locus_tag="AC16VR_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011171.1"
                     /translation="MKAVLQLFWQICRLKQSPEYVPTQTWFVLLVLAANLICSAAVSL
                     SAEGAPGLLTTINSIVVGQTTTAALVWVALNLRDMGGRFLATITALFGCDLIITAVFA
                     LILPLTLLAPTMAPVLFLLFVAWSVSVAGFILHRALQVPLALGIVVALGMSVLSLALS
                     QLAIGA"
     gene            2696..3958
                     /locus_tag="AC16VR_08175"
     CDS             2696..3958
                     /locus_tag="AC16VR_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014576136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR6011172.1"
                     /translation="MKRANAFYAQSGGVTAVINASACGVIQTARKHRDRIGKVLAGRN
                     GIIGALAEELIDTGRESAKDIAGLRTTPSGAFGSCRYKLKSLEENEREYRRLIDVFRA
                     HDIGYFFYNGGGDSQDTAHKVSQLGGRLGYPITCIGIPKTVDNDLPFTDCCPGFGSVA
                     KYVAVSIREAAMDVASMCETSTKVFILEVMGRHAGWIAAAGALAQEGPRDAPHIILLP
                     EVPFDRKRFLAKVRETVDGCGYCVIVVSEGAQYPDGTFLADAGSKDAFGHTQLGGVAP
                     TLAAMIKDELGYKYHWAVADYLQRAARHVASATDVEQAYAMGRAAVELALAGENAVMP
                     IVVRKSDSPYRWTVGSVKLSRVANVERKMPKSYISKDGFGITARGRRYLAPLIAGEDY
                     PAYRNGLPQYVRLKNELVAKKLEPFQLS"
     gene            complement(4010..6037)
                     /locus_tag="AC16VR_08180"
     CDS             complement(4010..6037)
                     /locus_tag="AC16VR_08180"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001960.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HIR6011173.1"
                     /translation="MNNIALIPPIFGIVGLIVAFFIFSLVKRYDEGTEEVKRIGDAIH
                     EGAMVFMRREYTMLAGFAAALIVILYIALGTETTIAFIVGALASATAGWIGMYTATKA
                     NVRTTTAAHTAGAAQALSVAFFGGSIMGLTVASMGLLGLGILYYVFGGDPETAHHIHG
                     FGMGASTVALFSRVGGGIYTKSADVGADLVGKVEAGIPEDDPRNPGVIADNVGDNVGD
                     VAGMGSDIFESYCGSMIATIAIASTLALAALTDLAPGMDEGSARAALMGLPLILASTG
                     LICSLVGIALVRLMSDKEPAVALRFGTLGAPVIFIIAAWFVIGQVGLESAVWWSVVSG
                     SVGGIIIGLVTEYYTSSKPIIRIAEAGETGAATVMITGLAVGMQSVVVPILAICGIIF
                     VSTHLAGLYGVGIAALGMLGTVGITMAIDAYGPVADNAGGIAEMSGLGKETRAITDSL
                     DELGNTTAAIGKGFAIGAAALAALAIIAAYVETISYQDPSFTLHIGDPEVLIGLFIGG
                     LIPFLIASITMTAVGDAAMEMIQEIRRQFREIPGLLEGKADPDNAKCIDIATQAALKR
                     MLLPGVIAVAVPPVVGFGLGPAALGGTLGGGLLGCVLLALTMANAGGAWDNAKKYVEK
                     GNLGGKGSDVHAATVVGDTVGDPFKDTSGPSMNILINVMAIVSLVIAPLLV"
     gene            complement(6220..6288)
                     /locus_tag="AC16VR_08185"
     CDS             complement(6220..6288)
                     /locus_tag="AC16VR_08185"
                     /inference="COORDINATES: protein motif:HMM:NF021600.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2333 family protein"
                     /protein_id="HIR6011174.1"
                     /translation="MASYVSRANAAIIDLRALLSQG"
BASE COUNT         1105 a         1962 c         2151 g         1071 t
ORIGIN      
        1 cccgtctacg cccagaccgg gctcgccggc catgacgcgc gctttgtcat cggcgacgtt
       61 cagccagccg gtgtggaaaa gcaccacgtc tcccgtgccg atggttacgc cctgggcctt
      121 ggctgcggcc ttgatctctc ggctgttgaa ggcggtgccg gcggggagaa tggccttgcc
      181 catgtgggcg gccatgtcga tcagcacgcc gcggccgacg atgggcggca ggcggtcgat
      241 gctcagcctc gacaggccgg ttgcgctcac gaaatcctgg gcccggttgc cgttgtagta
      301 cacgtgctcg atgcccatgt ggccgagccc gtcgatctgg ctgccgatgc ccatccagaa
      361 actcagcaga tcgtcgttgc cggacgccct gttggtaccc atcagggatc cggtgccgtc
      421 gccgggctgc agcacggtga gcgaatagct tcgcggcggg taggcgggcg agtcggggcc
      481 ggtctccacc cccagcgggt aggtcttgcc ttccgtgacc agacgggccg cctgaacgac
      541 cttctcagga gacaggttgt tgatggcccc cagggtgtcg tcggcgccgt agcgcgacgg
      601 gtaccaatcc gctgcgttgg cagcagggac gaatgcgagc agcagcgctg cgacgaagca
      661 tgccggtctg gattgcatgg cggggtctcc tggcgaaagt gggctggaag tggtgcccga
      721 gtttagccgc tgcgcagacg ggttgggagc ttttggtaga tcccgccgaa gctgccgttg
      781 ctcatgatca cgatgtggcg gcgcttctgg ggcagctggg ccagggcctc gatgagcttg
      841 tcgagctcga aattgatgtg cgcagggatc acgcagctct tcagcagctc cgagaggtcc
      901 cactgaatgt tctccccgcg aaaccagtag acctcgtccg ccggcgagca gcacgtggcg
      961 agctcgttac gcagggtccc cagtgacatg gtgtgggagc ggggctcgat gacggcgatg
     1021 atctcctcgc tgcctacctt gttgcgcagg ccctgcagcg ttgcgcggat ggcggtgggg
     1081 tggtgagcaa agtcgtcgta gatcaacgtt gcgccgatct cggcaatgag ctccaggcgc
     1141 ctcttgacgc cttcgaagct gcccagggcg tctatggcga cctccggcgg cacgccggcg
     1201 tgccgggcgg cagctatggc ggccagggcg ttgcggacgt tgaagtcgcc gaagatgggc
     1261 catcgcaccg tgcccagatt ttcctcgccc aggcagacgt cgaactcagc acctgtgccg
     1321 tctcccctgc tggcgctcca ccgctcaccg ttgtcgcgtt ccagcggttg cttcaccccg
     1381 ggggcgccca cgcgggcgat gggcgtccag caaccctgct ggagggtctc gttgacgttg
     1441 tcgtcgttgg acggcgcgat gacgagcccg tggccgggca cggtgcgcag caggtgattg
     1501 aactgggtct ggatggcggc gaggttgggg aagatgtccg cgtggtcgta ctccagattg
     1561 ttgatgatca gggtgcgcgg atggtagtga acgaacttgg atctgcggtc gaagtacgag
     1621 cagtcgtact cgtcggcctc caccacgaaa aaggggtcgt cgcccagccg ggacgagcgc
     1681 ggaaagttct tgggcacgcc gccgatcagg tagccgggct tcagccccgc ctggtcgaga
     1741 atccaggcca gcatgctggc cgtcgtggtc ttgccgtggg tgcccgtgac ggccagcacc
     1801 cagcggccgg gcatcacttg gctgccgagc cattcggcac cggacacgta gcgcagcccg
     1861 ttctcgatga tgtgctcgac gcccggatga cccctgggca ggcccgcgtt gccgatcacc
     1921 accagctccg gctcgggatc caggtgggcg ggatcgaagc cttcgaacac ctcgatgtcg
     1981 gcggccgcca gctgatcgct catgggcggg tagatggcca cgtccgaccc ggagacccgg
     2041 tatcccatct ccctggccag ctgggccagg ctccccatca gggtgccgcc gatgcccaga
     2101 aaatagattc ggcgttctgc gttcatgcgc cgatggccag ctggctcaat gccaggctga
     2161 gaaccgacat gcccagggcc acgacgatcc cgagcgcaag cgggacctgc aacgcgcgat
     2221 gcagaatgaa gccggcaacg ctcacgctcc aggcgacgaa gagcaggaac agcacaggcg
     2281 ccatggtcgg tgcgagcagg gtgagcggca ggatgagggc gaagactgcc gtgatgatga
     2341 ggtcgcagcc gaacagcgcc gtgatggttg caaggaaccg cccacccatg tcgcgcaggt
     2401 tcaacgccac ccacaccagg gccgccgtcg tcgtctggcc cacgacgatg ctgttgatgg
     2461 tggtcagcag gccgggcgcg ccttcggcgg acagagaaac ggcagcgctg cagatcaggt
     2521 ttgcggcgag gaccagcagc acgaaccacg tctgggtggg tacgtactcg ggactctgct
     2581 tcagccggca tatctgccag aaaagctgca agacggcttt catcggcgaa attgtcgcac
     2641 aaaactttct cttcacggac aatgaccgct cgttttccgg acgagacgtc gagggatgaa
     2701 gagagccaat gcgttctacg ctcagtcggg cggggtcacg gccgtcatca acgcatccgc
     2761 atgcggggtc atccagaccg cgcgcaagca tcgcgatcgc atcggtaagg tcctcgccgg
     2821 acgcaacggg atcatcggcg cgctcgccga ggagctgatc gacacgggcc gggagagcgc
     2881 caaggacatt gccgggctgc gcacgacgcc gagcggggct ttcggatcct gccggtacaa
     2941 gttgaagtcc ttagaggaga acgagcgcga gtaccggcgc ctgatcgacg tctttcgagc
     3001 ccacgacatt ggttactttt tctacaacgg cggcggggat tcccaggaca ctgcccacaa
     3061 ggtgtcccag ctgggcgggc ggctcggata tccgatcacc tgcatcggga ttcccaagac
     3121 ggtcgacaac gatcttccgt tcacggactg ctgtcccggc ttcggatccg ttgccaagta
     3181 cgtcgcggta tccatccgcg aagcggccat ggacgtcgcg tcgatgtgcg agacctccac
     3241 caaggtcttc attctggagg tcatggggcg tcacgcgggt tggatcgcgg cggcgggagc
     3301 cctggcccag gaagggccgc gcgatgcgcc tcacatcatc ctgctgccgg aggtgccctt
     3361 cgatcggaaa cgttttctcg ccaaggttcg ggagaccgtg gacgggtgcg gttactgcgt
     3421 aatcgtcgtc tccgaaggag ctcagtaccc cgacggcacc ttcctggccg atgccggaag
     3481 caaggatgcc ttcggccaca cccagctggg tggagtggcg ccgacgctgg cggccatgat
     3541 caaagacgag ctcggctaca aataccattg ggcggtggcc gactacctgc agcgggcggc
     3601 caggcacgtg gcctcggcca ccgatgtgga gcaggcttac gccatgggcc gggcggcggt
     3661 ggagctggcc ctggccggag aaaacgccgt catgcccatc gtggtgcgca agagcgattc
     3721 gccctatcgg tggacggtgg gcagcgtcaa gctttccagg gtggccaacg tcgagcggaa
     3781 gatgccgaag agctacatct caaaggacgg gttcggcatc acggcgcgag gcaggcggta
     3841 cctggcgccg ctcatcgccg gagaggatta ccctgcctat cgaaacggcc tcccacagta
     3901 cgtgcggctg aagaacgagc tcgtggcgaa aaagctcgaa cccttccagt tgagctgaag
     3961 ccggagagaa gatgggccgg ccccgcgtgc agcggggccg gcaggttcat cagaccagca
     4021 gcggcgcgat gaccaggctg acgatggcca tcacgttgat caggatgttc atgctgggcc
     4081 cggatgtgtc cttgaaggga tcgcctacgg tgtcgccgac caccgtggcg gcgtgaacgt
     4141 cagagccctt gccgcccagg ttgcccttct cgacgtactt cttggcgttg tcccaggcgc
     4201 caccagcgtt agccatggtc agggcaagca gcacgcagcc gagcagcccg ccgcccaggg
     4261 ttccgccgag agccgccggg cccaggccga agccgacgac gggcggcacc gccacggcga
     4321 tgacgccggg cagcagcatc cgcttgagtg ccgcctgggt ggcgatgtcg atgcacttgg
     4381 cgttgtccgg atccgctttg ccctccaaca gccccggtat ctcacggaac tggcggcgga
     4441 tttcctggat catttccatg gcggcgtcgc ccacggccgt catcgtgatc gaagcgatga
     4501 ggaatgggat cagccccccg atgaacagcc cgatcaggac ctcggggtcg ccgatgtgca
     4561 gggtgaagct cgggtcctgg taagagatgg tctctacgta agcggcgatg atggcaagtg
     4621 ccgccagggc cgctgcgccg atggcgaaac ctttgccgat ggcggcggtg gtgttgccga
     4681 gctcgtccag ggaatcggtg atggcccggg tttccttgcc cagaccgctc atctcagcaa
     4741 tgcctccggc gttgtcggct accgggccgt aggcgtcgat ggccatggtg attcccacgg
     4801 tcccgagcat gcccagcgcg gcgatcccga cgccgtaaag gccggcgagg tgcgtcgaca
     4861 cgaagatgat gccgcagatc gcgaggatgg gaaccaccac ggactgcatg cccacggcga
     4921 ggcccgtgat catcaccgtg gccgcgcccg tctcgccggc ctcggcgatc cggatgatcg
     4981 gcttggatga ggtgtagtac tcggtgacca ggccgatgat gatgccgcca acggagccgg
     5041 agaccaccga ccaccacact gcggattcca gacccacctg gccgatcacg aaccaggcgg
     5101 cgatgatgaa tatgaccggc gcgcccaggg tgccgaaacg cagggccacg gcaggctctt
     5161 tgtcggacat cagcctcacc agcgcgatgc cgaccagcga gcagatcagg cccgtggaag
     5221 cgagaatgag ggggaggccc atgagggccg cccgggcgga gccctcgtcc atgcccggcg
     5281 ccagatccgt cagtgctgcc agcgcgaggg tcgaggcgat ggcgatggtg gcgatcatgg
     5341 agccgcaata gctctcgaag atgtccgagc ccatgccggc gacgtcgccc acgttgtcgc
     5401 ccacgttgtc ggcgatcacg ccggggtttc gcgggtcgtc ctcggggatt cccgcttcga
     5461 ccttacccac caggtcggcg ccgacgtcgg cgctcttggt gtagatgccg cccccgaccc
     5521 gggagaagag cgcgaccgtg gaagcgccca tgccgaaccc gtgaatgtgg tgcgcggtct
     5581 ccgggtcgcc gccgaacacg tagtagagga tgccgagccc gagcaggccc atggacgcga
     5641 cggtgaggcc cattatggat cccccgaaga aggccacgga cagcgcctgg gccgcgccgg
     5701 cggtgtgggc ggccgtagtc gttctgacgt tggctttggt cgccgtgtac atgccgatcc
     5761 agcctgccgt cgcggaggcc agcgcgccga cgatgaaggc gatggtggtt tctgttccga
     5821 gggcgatgta gaggatgacg atcagcgcgg ccgcgaaccc cgccagcatc gtgtactccc
     5881 ggcgcatgaa gaccatggcc ccctcgtgaa tggcgtcccc gatccgcttg acttcctcgg
     5941 tgccctcgtc gtaccgcttc acgaggctga agatgaagaa ggctacgatc aggccgacga
     6001 taccgaatat tggcggtatc agcgcaatat tgttcattgg attccccaac tcgatgacca
     6061 tgtgtggaac gcgggcgatc tgctccaccc cgtgcttgtt gctgcacgat acgcgcggag
     6121 gcagcgctcg gttcggcgcg aattctaacg gagcggtctg cgaataagaa cctagcgcgc
     6181 ggcgggattt gtctgaattt gtatgggacc cgccgccggt cagccctggg agagaagcgc
     6241 ccgcaggtcg atgatggcgg cattcgcccg ggagacgtag ctcgccatg
//
LOCUS       DBNHQR010000365         5769 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1681070, whole genome shotgun sequence.
ACCESSION   DBNHQR010000365 DBNHQR010000000
VERSION     DBNHQR010000365.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5769)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5769
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1681070"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..1863
                     /gene="recN"
                     /locus_tag="AC16VR_08190"
     CDS             211..1863
                     /gene="recN"
                     /locus_tag="AC16VR_08190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIR6011175.1"
                     /translation="MLRELFVRDFALVESLEIGLCEGLTVITGESGAGKSILLGALGL
                     VLGDRADTDTIRPGAKRADVTAEFDLSGQPAAQAFLAEHDLEDADHPERCLVRRVVNQ
                     EGRSRAFINGAAVTRALLREFAEDLVDIHGQHEHQRLAEPATQLKLLDDYGVDAALLE
                     SCRESYRFWQRAVAEADALEAALASKQDRASLLSYQLEELAALGLEPGEFETLESEHK
                     RLSQAQDLRETVGHGLAVLEEADVLGRVSGALANLDDEHPALQAARDLLSSADSLVTD
                     AVRELRRYDDSLDVDEGQLGLLEERLTSVVDLARKHRVQPDRLVEHMALLQAELDAIS
                     TDRGSLDALREAAAGHEAEFRSTAGDVSLQRHEAAVHFASAVSGCMQNLGIGGGELRL
                     AFKEGESERGLESVEYQVMTNPKYPEAPLARIASGGERARISLAIQVVAAEKSALPCL
                     VLDEADVGIGGTTADVLGRLLRSLARHTQVICVTHAPQVAALGNHHLRVTKSSDQDTR
                     IEPLSEDTRIDEVARMLAGSGVTDKSRDYARTLLDEAHQALH"
     gene            1915..2316
                     /locus_tag="AC16VR_08195"
     CDS             1915..2316
                     /locus_tag="AC16VR_08195"
                     /inference="COORDINATES: protein motif:HMM:NF016258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990063 - Bam protein complex [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HIR6011176.1"
                     /translation="MVRLLASTLVLALLLAAGCSFRAPKFSLPRVHKITVQQGNVITQ
                     DMIDQLKPGMTRSQVAYVMGEPVFRNSFDESRWDYVYTVEVPGIFQDERRVSLYFEND
                     LLAYFTGDYAPSAAAGAAGAATDTSTAAEQG"
     gene            complement(2321..2590)
                     /locus_tag="AC16VR_08200"
     CDS             complement(2321..2590)
                     /locus_tag="AC16VR_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007900540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HIR6011177.1"
                     /translation="MKVDVVYALPDRQTQVGVSLADGATVADALGAVADTEPFSALDL
                     AAVPVGVFGESVGRDRQLRDGDRVEIYRPVLVDPKEARRRRAASA"
     gene            complement(2587..3918)
                     /locus_tag="AC16VR_08205"
     CDS             complement(2587..3918)
                     /locus_tag="AC16VR_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007349622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HIR6011178.1"
                     /translation="MWSSRWLFVLAAAGSAVGLGNIWKFPYIAGENGGGAFVIVYLVC
                     IALVGVPIMMAEVMMGREGRQSPINTMRTLTEAHGRSPLWVLIGWMGVLAGFLILSYY
                     VVIAGWALHYIWKMADGTFAGATADVANQTFNDFLADPWALMFWQTVFMAATVWIIAR
                     GVIKGLESAIRWFMPLLFVLLMVLLGYAVTSDGFSQGWDFMFDFNWSALDANAVLIAM
                     GQAFFTLSLGMGAIMAYGAYLPSGTSITTTVATIAGLDTLVALAAGLAIFPIVFANGL
                     EPGQGPGLMFVTIPLAFGQMPLGLVFGTVFFVLVSFAAITSAISISEPALAYLVEQYN
                     AKRSRVAITLGVIGWLLGVGTVLSFNHWADVHVVGELTYFEFVDYASQNVLLPLGGLF
                     IALFAAYALPRSVIGEQLGITDGWRALTWKFFCGLVAPLGVLAVFLYNFLA"
     gene            4043..4519
                     /gene="smpB"
                     /locus_tag="AC16VR_08210"
     CDS             4043..4519
                     /gene="smpB"
                     /locus_tag="AC16VR_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HIR6011179.1"
                     /translation="MNSKTGKPAPKTIALNKRAKRDYHFEQTFEAGLVLEGWEVKSLR
                     AGKAQLVDSYVLVRDGEAWLLGANISPLPSASTHVVADPQRTRKLLLHTKEIAQIFTA
                     TRQKGYACIATALYWKGNRVKCELALGKGKKLHDKRATERERDWNRQKQRIMKQAG"
     gene            complement(4516..5001)
                     /gene="ribH"
                     /locus_tag="AC16VR_08215"
     CDS             complement(4516..5001)
                     /gene="ribH"
                     /locus_tag="AC16VR_08215"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HIR6011180.1"
                     /translation="MAQTAIDINQLPMIENAGRVAILKSKWYPELVDSMARCCEGVLR
                     DRGIDRVEVHTLPGSLELPLAAADLLAADSGGDIDAIVCFGIILKGETLHFEMITNEC
                     MRGLGEVMQRYRRPVIVEILPVLEMAHAESRAADDEFNKGIEAAAAAIEMIAWRRAVG
                     A"
     gene            complement(5378..5575)
                     /locus_tag="AC16VR_08220"
     CDS             complement(5378..5575)
                     /locus_tag="AC16VR_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011181.1"
                     /translation="MSTKPRLAPAIPGSEADFASLMAQAPNSTALFFELYAEFWQNGV
                     VAEEIKEMTRMRNARITDCGY"
BASE COUNT         1087 a         1909 c         1862 g          911 t
ORIGIN      
        1 ggcgagcttc ttcgaggcaa ccgggctgcc gtccgccaga tagcgctcca cgagcagctt
       61 gaagagaagc tgcgcacggt cgtctacgtt gtcttctact tgctttctgg ccattttcgt
      121 tggctaatga ttacggtgcg gcgctctgaa ggcccacggt acaggcgttc tgacagtcca
      181 ctatgaacct aattcatccc gcgtcaagcc atgcttagag agctcttcgt aagggatttt
      241 gctctggtgg agtcgctgga gatcggtctt tgcgaggggc tgaccgtgat caccggcgag
      301 tcgggagcgg gcaagtccat actgctcggc gccctggggc tggttctagg cgatcgggca
      361 gacacggaca ccatcaggcc cggcgccaag cgggcggatg tcaccgccga gttcgacctc
      421 agcggccagc ccgccgctca ggcgtttctt gccgagcacg acctggagga cgccgaccat
      481 cccgaaagat gcctggtccg tcgggtggtc aaccaggagg gtcgctccag agccttcatc
      541 aacggcgccg cggtaacccg cgctctgctg cgcgagttcg ccgaggacct cgtggacatt
      601 cacggtcagc acgagcacca gcgcctggcc gagccggcca cccagctcaa gctcttggac
      661 gattacggcg tggacgccgc gctcctggag tcgtgccggg agagctatcg cttctggcag
      721 cgggcagtgg cagaagcgga cgccctcgag gccgccctgg caagcaagca ggaccgcgcc
      781 tcactgctct cttaccagct cgaggagctg gccgcgctgg ggctagagcc aggggagttc
      841 gaaaccctgg aaagcgagca caaaaggctc tcccaggccc aggacctgcg cgagaccgtc
      901 ggccacggcc tcgcggtgtt ggaggaggcc gacgtcctgg gccgcgtctc gggcgcgctg
      961 gccaacctgg atgacgagca tccggctctt caggctgcac gggatctgct gtcatccgcc
     1021 gacagcctcg tgaccgacgc ggtacgcgag ctgcgccgat acgacgattc cctggacgtg
     1081 gatgagggcc agctcgggct gctggaagag cggctgacga gcgttgtcga cctcgcccgc
     1141 aagcatcggg ttcagccgga ccggctggtc gagcacatgg ccttactgca ggccgagctc
     1201 gacgcgatct ccaccgaccg cggctccctc gacgccctgc gcgaggcggc cgccggacac
     1261 gaagccgagt tccgctcaac cgccggcgac gtgtctctcc agcggcacga ggcggccgtt
     1321 cactttgcct cggccgtcag cggttgcatg cagaatctgg gtatcggcgg cggcgagctg
     1381 cgcctggcct tcaaagaggg cgagtccgag cgtgggctgg agagcgtgga gtatcaggtg
     1441 atgaccaacc ccaaataccc ggaggccccg ctcgcccgga tcgcctccgg cggcgagcgt
     1501 gcccgcatca gcctcgccat ccaggtggtg gccgctgaga agtcggccct gccgtgcctg
     1561 gtcctggacg aggcggacgt gggcatcggc ggaaccacgg cggacgtgct cggaaggctc
     1621 ttgagatctc tggcacggca cactcaggtc atctgcgtca cccacgcccc ccaggtcgcc
     1681 gccctgggca atcatcacct gcgggtgacc aaatccagcg atcaggacac gcgcatcgag
     1741 cccctttccg aggacacccg aatcgacgag gtcgcgcgga tgctggcggg ttctggcgtc
     1801 accgacaagt cgcgggacta tgcccgcacc ctgctcgacg aagcccatca ggcccttcat
     1861 tagcccgcga gatctgggta acatccctgg ccttcgcgca aacaccctga ggccatggtc
     1921 cgactgctcg cttccacact cgtcctcgcg ctgctccttg cagccggctg cagcttccga
     1981 gcgcccaagt tcagcctgcc tcgcgtgcac aagatcaccg tccagcaggg aaacgtaatc
     2041 actcaggaca tgatcgacca gctaaagccg ggcatgaccc gcagccaggt cgcctacgtc
     2101 atgggggagc cggtcttccg caactcgttc gacgagagcc gctgggacta cgtctacacg
     2161 gtggaagtgc ccggaatatt ccaggatgag cggcgggtga gcctgtactt cgaaaacgat
     2221 ctgttggcct acttcaccgg cgactacgcg ccgtccgccg ccgccggcgc ggccggagcc
     2281 gcgaccgaca cctccacggc agccgagcag ggatgaccat tcaggcggag gctgcccgcc
     2341 gccgcctggc ttccttggga tcaaccagca caggccggta gatctcaacg cggtccccgt
     2401 cccgcagctg cctgtcccgg ccaacgctct ccccgaatac ccccacggga accgcggcca
     2461 gatccagcgc cgagaaaggc tcggtatcag cgacggcccc aagggcgtct gctaccgtgg
     2521 cgccatccgc caggctaacg cccacctgcg tctgccggtc cggcagcgcg tagaccacgt
     2581 cgaccttcat gcgaggaagt tgtagagaaa tacggccagc acccccagcg gcgccaccag
     2641 gccgcagaag aacttccacg tgagcgcgcg ccagccgtcc gtgatgccga gctgctcgcc
     2701 gatgacggac ctgggcaggg cataggcggc aaacagggca atgaacagcc cgccgagcgg
     2761 cagcaggacg ttctgcgatg cgtagtcgac gaactcgaag taggtcagct cgccgaccac
     2821 atggacgtct gcccaatggt tgaaggagag caccgtgccc acgcccagca gccagcctat
     2881 gacgcccagc gtgatcgcca cgcgggaccg cttggcgttg tactgctcca cgaggtaggc
     2941 aagggcgggc tcgctgatgg agatggcgga ggtgatggcg gcaaagctca ccagaacgaa
     3001 gaacacggta ccgaagacca ggcccagcgg catctggcca aaggccagcg ggatggtgac
     3061 gaacatgagc cctgggccct ggccgggctc caggccgttg gcgaacacga tgggaaagat
     3121 ggccagcccg gccgccaggg cgaccagggt gtccaggccc gcgatggtcg ccacggtggt
     3181 ggtgatggac gtgccgctcg gcaggtacgc cccgtaggcc atgatggcgc ccatcccgag
     3241 gctcaaggtg aagaaggctt ggcccatggc gatcagcacc gcgttggcgt ccagggcgga
     3301 ccagttgaag tcgaacatga agtcccagcc ctgggagaac ccgtcggagg tcaccgcgta
     3361 accgagcagc accatcagca ggacgaacag cagcggcatg aaccaccgta tggccgactc
     3421 gagacctttg atcacgccgc gggcaatgat ccagaccgtg gccgccatga agaccgtctg
     3481 ccagaacatg agcgcccagg gatcggccag gaagtcgttg aatgtctggt tggcaacgtc
     3541 ggcggtggct ccggcgaacg ttccgtcggc catcttccag atgtagtgca gggcccatcc
     3601 ggcgatgaca acgtagtagc tgagaatgag aaacccggcc agcacgccca tccagccgat
     3661 gagcacccag agcggcgagc ggccatgggc ttcggtgagc gtgcgcatgg tgttgattgg
     3721 gctctggcgg ccttccctgc ccatcatcac ctcggccatc atgatgggca cgccgacgag
     3781 ggcgatgcag acgaggtaga cgatgacgaa cgcgccgccg ccgttctccc cagctatgta
     3841 agggaacttc cagatgttcc cgaggcccac ggccgatccc gctgctgcca gcacgaacaa
     3901 ccatctgctg gaccacatgc catgccgaga ttcggtagcc ggggccatga gcacttccaa
     3961 ggcgatcgcg ttgggccggc attgtggccc aacgtctttg gggcctcaac cgcatataat
     4021 ccgaggccat gaggtcctcg atttgaacag caaaacgggc aagcccgcac caaaaaccat
     4081 cgcgctgaac aagcgcgcga agcgcgacta ccacttcgag cagaccttcg aagccggcct
     4141 ggtgctcgag gggtgggagg tgaaaagcct gcgcgcgggc aaggcgcagc tggtggacag
     4201 ctacgttctc gttcgcgacg gcgaggcgtg gctcttgggc gccaacatct caccgctgcc
     4261 cagcgcatcg acccatgtgg tcgcggatcc tcagcgcacc cgcaagctgc tgctccacac
     4321 gaaagagatc gcgcagatct tcacggccac ccggcagaag ggctacgcgt gcatcgccac
     4381 agcgctgtac tggaagggca accgggtcaa gtgcgagcta gcgctgggca agggcaaaaa
     4441 gctgcatgac aagcgggcca ccgagcgcga gcgtgactgg aaccgccaga agcagcggat
     4501 catgaagcag gcgggctaag cgcctacggc ccggcgccag gcaatcatct cgatggcggc
     4561 cgcagccgcc tcgatccctt tgttgaactc gtcatcggcg gcccgtgatt ccgcatgggc
     4621 catctccagt acgggcagga tctccacgat gaccggccgc cggtatcgct gcatgacctc
     4681 gccgaggccg cgcatgcact cgttggtgat catctcgaag tgcagggttt cgcccttcag
     4741 gatgatgccg aaacagacga tcgcgtcgat gtcgccgccc gagtcggcag caagcaggtc
     4801 ggcggcggcc aggggcagct ccagggatcc cggcagcgtg tgaacctcca cccggtcgat
     4861 gccccggtcg cgaaggaccc cctcgcagca gcgcgccatg ctgtccacca gctcgggata
     4921 ccacttggac ttgaggatcg ccacccgccc ggcgttctct atcatcggca gctggttgat
     4981 gtctattgcg gtctgcgcca tgtcggcaag ctctgtgtca gcggagggcg aagaataccc
     5041 gaatgagccg acgcgggccc gttcaggacc ccggtgtcgg cacgacggtc accggcatct
     5101 cctcgggctc cagcccgagc atgatgagca ccttggacat gccgagaaag cagccgacgc
     5161 ccagcgccat ctcgacgatc tgcgcatcgg agaagtgagc tttgagagcg gccttggcat
     5221 ccgcatcgag tgggccagaa gccccgatga tggcgtccgt cagcttcagg gccgccgtct
     5281 cggccggcgc gagcgagcgc tcgtacccgt ccgcgatctg ctcgaccttc gcttcgtcca
     5341 gccccgccgc acgggcggag tcgaatcgta cgttttttca gtagccgcag tcggtgatgc
     5401 gcgcgttgcg catgcgcgtc atctccttga tctcttcagc gacgacgccg ttctgccaga
     5461 actcggcgta gagctcgaaa aaaagggcgg tggagttggg cgcttgagcc atgagcgatg
     5521 cgaaatcggc ttcactcccg gggattgcgg gtgccagtcg aggcttggtg gacatcggat
     5581 gctcccctga tcagaccatg gcgccgtcgg caaggggcgg gctcgcgagc aaagcgctct
     5641 cctcgtccag ctccagcgtg agcctgagcc tgcagacgac gtcgaagaaa gcgagcgccg
     5701 tcagcaggga caccgtgccc ggcccaccca ggagcccctc tgcatgcaca acctcttcat
     5761 cagtcagct
//
LOCUS       DBNHQR010000366         2194 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_288341, whole genome shotgun sequence.
ACCESSION   DBNHQR010000366 DBNHQR010000000
VERSION     DBNHQR010000366.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_288341"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /locus_tag="AC16VR_08225"
     CDS             complement(<1..1019)
                     /locus_tag="AC16VR_08225"
                     /inference="COORDINATES: protein motif:HMM:NF019640.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6011182.1"
                     /translation="MHEREFVERIRGLVPMLEAHAGEAERDRKPVDSVMAAVEETGAY
                     RFFVPKRYGGWEFGLDTFMDVGLLLGEGCVSTAWVVTFCMEHNWLLGLFNQQAQDEIF
                     GEFPYIIAPGTLAPRGTAEPVDGGYRVSGRWQWGTGVMHANWVMVGALTPSADPEKPD
                     LCMYLIPREEVDVLDTWHVAGMVGTGSNDIEVKDVFVPGHRMQSVSHMRDGGSPGAAL
                     HGTPTYRMPMMPVLGLTAAAPAVGGARRAVALFRELLGERNVYGTTEKQGQRAIAQAR
                     LAHAQIAVENAQILLKDTARRVMEWGESGERCPDVERAHLRLKIATVVRQARDVVQQV
                     VEACGA"
     gene            complement(1031..1489)
                     /locus_tag="AC16VR_08230"
     CDS             complement(1031..1489)
                     /locus_tag="AC16VR_08230"
                     /inference="COORDINATES: protein motif:HMM:NF024212.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR6011183.1"
                     /translation="MADLVRALDWFDNGLQNVLASLGYKPLHRTQSMIMVHVASGVES
                     PADIARSMGLSRQNVHHMAKALIADGLIEQAPDPADPRRTLYRLAGSAAEIRRAALET
                     LAQLELVLAGRIGLRSLHALRGALGKDWGDAIAGGEALAAELDGHDVLAN"
     gene            1780..>2194
                     /locus_tag="AC16VR_08235"
     CDS             1780..>2194
                     /locus_tag="AC16VR_08235"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR6011184.1"
                     /translation="MASWQRQFVSTTSPTGTRNGAGFYPCQGLYYAPTGSRPRTALIA
                     THYNVDFSEHYLGEFMAARGYGFLGWNTRFRGAEAFFLLEHALVDIGTGVRWLKEEAG
                     VERVVILGNSGGGSLMGAYQSQALGVTMTATPGLSL"
BASE COUNT          358 a          777 c          686 g          373 t
ORIGIN      
        1 gcgccgcagg cttccaccac ctgctgaacc acgtcgcggg cctggcggac gacggtggcg
       61 atcttcaggc gcagatgggc acgctcgacg tcggggcaac gctccccgct ctctccccac
      121 tccatcaccc ggcgcgcggt gtccttgagc aggatctggg cgttctcgac ggcgatctga
      181 gcatgggcga gccgggcttg agcaatggca cgctggccct gcttttcggt cgtgccgtag
      241 acgttgcgct cgccgagcag ctcgcgaaag agcgccaccg ccctgcgcgc gccgcccacg
      301 gcgggcgcgg cggccgtcag gcccaggacg ggcatcatgg gcatgcggta cgtcggggtg
      361 ccgtgcagcg ccgcccccgg gctgccaccg tcgcgcatgt gggacacgct ctgcatccgg
      421 tggccgggga cgaagacgtc cttcacctcg atgtcgttgc tgccggtgcc taccattccc
      481 gccacgtgcc aggtgtcgag gacgtccact tcctccctgg gaatcaggta catgcacagg
      541 tcgggctttt ccggatccgc cgacggcgtg agcgctccca ccatgaccca gttggcgtgc
      601 atcaccccgg tcccccactg ccatcgaccg ctcacacgat agccgccgtc caccggctct
      661 gcagttcccc gcggcgcgag ggtgccggga gcgatgatgt agggaaactc accgaagatc
      721 tcgtcctggg cctgctggtt gaacaggccc agcagccagt tgtgctccat gcagaaggtc
      781 accacccagg cggtggatac gcagccttcg ccaagcagga ggccgacgtc catgaacgtg
      841 tccaacccga actcccagcc gccgtagcgt ttcggcacga agaaccggta cgcgccggtt
      901 tcctcgaccg ccgccatcac ggaatccacg ggcttgcgat cgcgctcggc ctcgccggca
      961 tgggcctcca gcatcggcac cagcccgcgg atgcgctcga caaattcccg ttcgtgcata
     1021 cccgcctacc tcagttagcc aacacgtcgt gcccgtcgag ctctgccgcg agcgcttcac
     1081 cgccggcaat cgcgtcgccc cagtctttgc cgagggcgcc ccgaagcgca tggaggctgc
     1141 ggagcccaat gcgtccagcg agcacgagct cgagctgagc cagagtctcc aaagctgcgc
     1201 ggcggatctc cgcggcggag cccgccagcc gataaagtgt gcgccgcggg tccgccgggt
     1261 ccggcgcctg ttcgatgagc ccgtcggcga tgagcgcttt ggccatgtga tgcacgttct
     1321 ggcggctcag gcccatcgat cgggcgatgt ctgccggcga ctcgaccccg ctggcgacgt
     1381 gaaccatgat catggactgg gtccggtgca gcggcttgta gcccagcgag gcgagcacgt
     1441 tctgaaggcc gttgtcgaac cagtcgagcg cgcgcaccag atcggccatc acgccaaccg
     1501 gttcgttgct gctttgcagc gttcgcgact gcccaatcat cgttaacccc gaatttccgg
     1561 atctgatctt cccggtgccg acgtcgccat cgagcacttt ctcacgattc gccgagggcg
     1621 aatcgggccg cgcgcaaagc atccagcccg cccagttagc gtcaaatgat cttacacaga
     1681 accacgggtc ccgaacagcg tcgccagagc caacgccgct ttgaaagctc cggatatatc
     1741 ccgtaatgtc ggtgcctcag gcttccgggg ggatcgccaa tggccagttg gcagcgccag
     1801 ttcgtttcca cgacctcacc gaccggcacc cgcaacggcg ccggttttta tccctgtcag
     1861 ggtctgtact acgccccgac gggctcgcgg ccccggacgg ccctgatcgc gacgcactac
     1921 aacgtggact tcagcgagca ctacctgggc gagttcatgg cagcacgcgg gtacggcttc
     1981 ctgggctgga atacccgttt ccgcggagcg gaagccttct tcctcctgga acacgctctc
     2041 gtcgacatcg gaaccggcgt gcgctggctg aaagaagaag ccggcgtgga gcgggtggtc
     2101 atcctcggca actccggcgg cggctccctg atgggcgcct accagtccca ggcactcggc
     2161 gtaaccatga cggcgacccc cggtctgtcc ttgc
//
LOCUS       DBNHQR010000367        14432 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_668632, whole genome shotgun sequence.
ACCESSION   DBNHQR010000367 DBNHQR010000000
VERSION     DBNHQR010000367.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 14432)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14432)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14432
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_668632"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="AC16VR_08240"
     CDS             <1..463
                     /locus_tag="AC16VR_08240"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6011185.1"
                     /translation="YRANRLIHTTLDEYEQVMAVNATGVFLGLRAVGREMKKAQAGSI
                     VNISSYAGMAAAAGSFAYGASKWAVRGMTKTAAVELARSGVRVNSIHPGAIDTEMLSE
                     AGDPAKLVRGTPLGRVADADEVASMALFLASDESSYSTGAEFLVDGGLFAI"
     gene            complement(475..1434)
                     /locus_tag="AC16VR_08245"
     CDS             complement(475..1434)
                     /locus_tag="AC16VR_08245"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6011186.1"
                     /translation="MQRTVLGRTGFEVSVAGLGCGGYSRLGQAYGKSAAHSEALVAAA
                     LDLGVNFIDTAPAYRTEGILGRALRGRRESAVVSTKALIHLNTEHPPKPSRFITGAEL
                     EARLDRSLEALGTDYVDVYNLHGIVEPELDYVDAELVPKLLDLKAAGKIRSLGITEHF
                     QVDTRHEMLARAVRSGVWDVVMVGFNLLNPCARQNVLAPARAMNVGVLNMHAVRTSLS
                     DPDRLTEALETAGDAGQLDLPPAGTAAALQSLVSESGAGSLTELAYRFCRHEPGVHVV
                     LTGTGKLDHLRANIAAIDNDPLPAPVSAGLEGLFGSVDCLSGE"
     gene            complement(1439..2338)
                     /locus_tag="AC16VR_08250"
     CDS             complement(1439..2338)
                     /locus_tag="AC16VR_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011187.1"
                     /translation="MTGNSSILSDLPATAGVARSTRREGVDVSLNAPTPAQPAIASPE
                     WAVAGWIIRFVRLMAGHRLAIPQEPGDVFLKVVAGALTDRGAFARPREIRDTLLLSEE
                     VTAGHDGALVAVMTRGDGAPERVEVMSELTLHGPLEDAFRWQRFDEKFGHVTHAFDGV
                     EAYMSPGFHLLEEDGDEVCYVNLWTSGKGVDLTTHNHANDPSPAAPAFAETHWVFYNG
                     SGRGGMYGCDEPGAPRTRYPMQTGQEHGPFFAVDEHGAPRRRENGAVAYPWHGWESGE
                     DDRAEQAFDFVAAFETNPDYVKI"
     gene            complement(2335..3903)
                     /locus_tag="AC16VR_08255"
     CDS             complement(2335..3903)
                     /locus_tag="AC16VR_08255"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HIR6011188.1"
                     /translation="MAKGERRQASMYPGSWAQIKGEDVAVLAAGSGDALTWRDLDRRS
                     NQAAHALRRAGLGAGDHLALIMENHLQFFEIAWAAIRSGLYITCVNRYLTAEEAAYIV
                     TDSSAKAVIVSAEIGLGSKLLPLIAECPVRLMARGVEPGWTSYEKTIEACPFTPVADE
                     TVGDSMLYSSGTTGRPKGIKRPLTGQSPAKGIPGVEQMSPYGFSQESVYLSPAPLYHA
                     APFAFCMRTLSLGGTVIMMERFDPLEALGCIERYRVTHSQWVPTMFVRLLNLDDEART
                     GFDLSSHRCAVHAAAPCPVPIKQQMMTWWGPIIWEYYAGTERNGSTLIDPHDWLAHPG
                     SVGRARSGVIHICDDDGRELDPGQEGLIYFEQPERPFEYHNAPDKTANATHPDHGNWT
                     ALGDVGYLDDEGYLYLTDRKAFVIISGGVNVYPREIEDVLIVHPKVADVAVFGVPNEE
                     FGEEVKAVVQPAPGTSPGDALAAELVGHARAHLAGFKVPRSIDFIDEMPRLPTGKLYK
                     QPLRARYWPERKST"
     gene            3990..5087
                     /locus_tag="AC16VR_08260"
     CDS             3990..5087
                     /locus_tag="AC16VR_08260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6011189.1"
                     /translation="MHVGFAPVFQNLGKPVPDHQVYAQELAMARAAEPMGFDSVWSVE
                     HHFTDYTMVPDVTQFLSYMAACTERVQLGSMVIVLPWHDPVRVAEEIAMLDALSGGRM
                     ILGIGRGLGRVEFDGFRVPMDESRERFVESATMILEGLERGFVELDGKHFQQPRRDIR
                     PAPFRTFRGRTFAAAVSPESMPVMARLGVGLLVIPQKPWESVRTDFEVYHRVWAETHG
                     AQSSPPDPMCGGFFFVDESADRAEEMAYKYIGRYYHSVMDHYELRIGHLEGTKGYEFY
                     EKIHEHIETRSDDGAARDFVRLMPFGTPEQVIEKVEFIHRMIGAKGVMTHFAYGGMDY
                     AEANRNMRLFADRVMPELQRLGEQRGALAVA"
     gene            complement(5130..5456)
                     /locus_tag="AC16VR_08265"
     CDS             complement(5130..5456)
                     /locus_tag="AC16VR_08265"
                     /inference="COORDINATES: protein motif:HMM:NF024802.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HIR6011190.1"
                     /translation="MTVARQRLDKIEEAIRAERGNEGPFFNGPDLSLVDAAYAPFFQR
                     FLIVEQWLATGLLESYPLVGAWADALAASPAVANSVPDVFHDEFVANLKRRGFHVAAL
                     MDAAAG"
     gene            complement(5595..5768)
                     /locus_tag="AC16VR_08270"
                     /pseudo
     CDS             complement(5595..5768)
                     /locus_tag="AC16VR_08270"
                     /inference="COORDINATES: protein motif:HMM:NF046279.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
     gene            5957..7138
                     /locus_tag="AC16VR_08275"
     CDS             5957..7138
                     /locus_tag="AC16VR_08275"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6011191.1"
                     /translation="MQVSRALIVGGGIGGLTAALCLARQGIAVSVFEQTADLAEVGAG
                     IQLSPNASRVLHHLGLEGALGSVAFLPRAVEMRDWRSGRLLASSPIDEAFRDRFVYPY
                     YHVHRADLLDVLERALRRAPGIELRTGARVEGIEPGDSAVFVEAGGERHGGDLLIGAD
                     GIHSTVREALFGAEAPRFTGNVAWRGLVPAGAVPSGFVRPAASAWWGPHKHFVHYYVR
                     RGELINCVGVVEKRGWEVESWTEPGDLSELQADFAGWHETVRRLIDNMDPQACFKWAL
                     FDRPPMSAWSDGRVTLLGDACHPTLPFMAQGAAMAIEDAAVLARCLVEGDGVSGSLRR
                     YESLRRARTARVQAGSRRNARLFHSSGPAAGLRNLIVGRASGRVMDWLYGYDALDPGA
                     P"
     gene            complement(7158..8750)
                     /locus_tag="AC16VR_08280"
     CDS             complement(7158..8750)
                     /locus_tag="AC16VR_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007155086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6011192.1"
                     /translation="MNTKPLDAIVVGAGFAGLYMLHRLRGLGLKTRVFEAGSDIGGTW
                     FWNRYPGARCDIESMEYSYQFSDELAREWEWSERYATQPEILGYINHVAERFDLRGDI
                     QLNTRVERARFSEETNRWTVTTSDGGEHEAKYLVMATGCLSSTNLPEFDGIDSFQGDW
                     YHTGQWPHEGVDFTGKRVAVIGTGSSAIQSIPLIAEQAEQLTVFQRSPNFSVPAHNRA
                     LDPGAVARIKARYPEFRDANRQTAFHADFRYNESNALDVSAEQREREYEARWERGGLP
                     FLAAFADLVFDADANETAAEFIRGKIRETVKDPDTAELLCPTSVVGCKRLCVDTDYYA
                     TFNRENVTLVSVRDTPIEAITPKGLRTADEEYEFDAIVFATGFDAMTGALMKMDIRGI
                     GGRSIQDAWSEGPKTYLGLAVSGFPNLFTVTGPGSPSVLTNMLPTIEHHVDWIADCVA
                     YMEHEGMSRIDAMPEAQTAWVAHVNEVGDSSLYPRCNSWYLGANIPGKPRVFMPYLGF
                     PPYAEKCREVAESGYDGFALSA"
     gene            9023..9466
                     /locus_tag="AC16VR_08285"
     CDS             9023..9466
                     /locus_tag="AC16VR_08285"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6011193.1"
                     /translation="MPGESCRVGYRHAMDAVPGSVWRTLADFGSLLSWVAGGDEGRIE
                     LSGEGIGMTRDLWLPSVGRVQHRLDVLDEAGRCLTYSLAEGRPLGMRSYSVTVSVEAA
                     GSGCTLAWQGAFVPEPGADSDAMAENLRRAYVDMSRRLDVLLRAA"
     gene            complement(9533..10729)
                     /locus_tag="AC16VR_08290"
     CDS             complement(9533..10729)
                     /locus_tag="AC16VR_08290"
                     /inference="COORDINATES: protein motif:HMM:NF024241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6011194.1"
                     /translation="MDHHRPNVTTTIDRSPTRPDKAADTTLKALFLLTAVLMVGYGAV
                     FSLLAEIRDAFGFTPTGIGLIAGSAFAAGFFAQLWLARYADRGYGAIMLRAGLAISAL
                     STLWMAVADTLAAWIAARALLGFGAGCVRPAVRRLALTADPANAGHAMGRLTAYETAG
                     FLLGPVLAALLDRWAGLASTFIVMTGVLALLAPMVWRVSIPGASRPTTGPVIGTLLRN
                     PYMQACLALGIAFWITIGVFEAVWAIFLADLGASQMFIGLTMSLFGIPMIFIAPRAGS
                     LAQHRGPLRVASVSMAVATVCMVLYGFLDSLWLLCVPLGIHAIADAYTMPASQLAVGR
                     ASGEGALAAGQGLFGATGMAVGAVTAAGGGVLYQSLGPSGLWWGSAGAMIVCLLISWW
                     RSPRAT"
     gene            10779..11510
                     /locus_tag="AC16VR_08295"
     CDS             10779..11510
                     /locus_tag="AC16VR_08295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6011195.1"
                     /translation="MNDNAIALVTGGATGIGAEVCRQLAAAGARVAICDVNEADGAPL
                     AESLGGEFLFCDVADLASVEGAVAGCCARLGVPDYVHLNAGVMTVGRYDAYLALEDVS
                     VEQYRRIIGVNVDGVFNGIKALLPPMRQKGGAITVTASIAGFAPMAIDPLYGATKAAL
                     ISLVRSVAAANEGGSVRINAICPGVVDTAIVPDAMSGTIPMMPPSAMAAEILDLLSDG
                     PSGEIRVKLNEETPAFHVEPSTLGR"
     gene            11525..12010
                     /locus_tag="AC16VR_08300"
     CDS             11525..12010
                     /locus_tag="AC16VR_08300"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6011196.1"
                     /translation="MNFDDYHAIVRRRYEYALGIDTRDWVLYRSIFTDEITMDFSSYN
                     GRPASGMAADDWVAGCKVLFAGLDATQHVMSNPMVDVDGDNARLRMYMKAEHFLRNDQ
                     GGMDFTIGGYYDDRLVRTDEGWKMQAVTLNVFWSRGNRQIMALAAEIGKGKLEEASSG
                     S"
     gene            12034..13317
                     /locus_tag="AC16VR_08305"
     CDS             12034..13317
                     /locus_tag="AC16VR_08305"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6011197.1"
                     /translation="MNDPESIANLEARMAWEQERAVPPEGFPALPPLPPGRYTSPAFF
                     DLERRHLWSKVWLYAAHDSQLPEPGSFLLLDIPDAPIFLIRGRDASVRAFYNVCSHRG
                     APLVREASGQVARLRCTYHQWTYDTDGRLIAVMDERDFAEPFDRSCLGLKEVRCEQWG
                     RWIFINEDPEAQPLLEWLDMIAAEFEQFDIERLRPAAIKSYELECNWKLTMDAFLEVY
                     HIQGIHPKTVGPSLDHRGAVMGLLPNGHTRMTCPTRMSRTETADTRGYTDSAGEAMPA
                     IPAGEIARNYHVSHNVFPNLITPVGDTARQFLMFWPLSLTRTRVDVVHFGLDWGEGER
                     PSGWDGLLAFWDVVMAEDLQFLEWQQKAVLSPGFQGYRLSYMERRIYYAHESIDRIIG
                     VENIPEALRAVPMLDAYRERAEDRCADQPSSPSSR"
     gene            complement(13354..13704)
                     /locus_tag="AC16VR_08310"
     CDS             complement(13354..13704)
                     /locus_tag="AC16VR_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011198.1"
                     /translation="MTAAREFYVLVGLMVAATVPMIVEIRADHGVGVLQVAAQIWTDA
                     LSAFTGDILNRRPVDRATLTGPVLALRLAYCLGPIVFYGLTLKLIWSYPLTPVRHARL
                     RERIERRNARRTAR"
     gene            13985..>14432
                     /locus_tag="AC16VR_08315"
     CDS             13985..>14432
                     /locus_tag="AC16VR_08315"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6011199.1"
                     /translation="MKVSIGIGGAASGGRRDFEDQVAYVVEAEALGVDTVWTAEAWGQ
                     DAISPLAYLAAKTQRIRLGTGIMQISARTPSMTAMTALTMAAISNDRFILGLGASGPQ
                     VVEGLQGQPFKAPLTRMRETVDIIKLAFAGEKLAYDGKYHRLPLPGG"
BASE COUNT         2393 a         4583 c         4965 g         2491 t
ORIGIN      
        1 ctaccgggcg aaccgcctca ttcacacgac gctcgacgag tacgagcagg tgatggccgt
       61 gaacgcgacc ggtgtctttc tggggctgcg agcggtgggc cgggagatga agaaggcgca
      121 ggcgggatcc atcgtcaaca tctcctcgta cgccggcatg gcggcggcag ccggctcgtt
      181 cgcctatggc gcgtccaagt gggccgtaag gggcatgacc aagaccgccg ccgtggagct
      241 ggcccgctcc ggcgtgcggg tgaactctat ccatcctgga gccatcgaca cggagatgct
      301 gagcgaggcg ggagatccgg cgaagctcgt gcgaggcacg cccctcggtc gggtcgccga
      361 cgcggacgag gttgccagca tggcgctgtt tctcgccagt gacgagagct cctactcgac
      421 gggcgcggag tttctcgtcg acggcggtct gtttgccata tagccgggtc cggctcactc
      481 gccggacagg cagtcgacgg atccgaagag gccttccagg ccggcggaga ccggcgcggg
      541 cagggggtcg ttgtctatgg cggcgatgtt cgcccgcaga tggtcgagct tgcccgtgcc
      601 cgtcagcacc acgtgcacgc cgggctcgtg gcgacagaag cggtaggcga gttcggtcag
      661 cgagccggcg ccggattcgc tcaccaggct ttggagcgcg gcggcggtgc cggccggggg
      721 cagatcgagc tgtcccgcgt ccccggcggt ctcgagcgct tccgtgagcc ggtccgggtc
      781 gctcagcgag gtgcgcacgg cgtgcatgtt gagaacgccg acgttcatcg cccgggctgg
      841 cgccagcacg ttctgccggg cgcaggggtt gagcagattg aaccccacca tgacgacgtc
      901 ccacaccccc gacctcaccg ctcgggccag catctcgtgt cgcgtatcga cctggaaatg
      961 ctcggtgatg ccgaggctgc gaatctttcc ggcggctttc aggtcgagca gcttggggac
     1021 gagctcggcg tcgacgtagt cgagctcggg ctcgacgatg ccgtgaaggt tgtagacgtc
     1081 gacgtagtcc gtgcccaggg cttccaggct tctgtccagg cgcgcttcca gctcggcacc
     1141 ggtgatgaac cgtgacggtt tcggcggatg ctccgtgttg aggtgaatga gcgcctttgt
     1201 cgagaccacg gcggattcgc gtctgccacg cagagcgcgg ccgaggatgc cctcggtgcg
     1261 gtaggcggga gcggtgtcga tgaaattgac gccgaggtcc agcgcggcgg cgacgagcgc
     1321 ctcgctgtgc gcagcgctct tcccgtacgc ctggccgagc cggctgtagc cgccgcagcc
     1381 gaggccggcg acgctcacct cgaaacccgt tcttcccagc accgtgcgct gcatgggatc
     1441 agatcttcac gtagtccggg ttcgtctcga aggcggcaac gaagtcgaag gcctgttccg
     1501 ccctgtcgtc ttctccggac tcccacccgt gccagggata cgccacggcg ccgttctcgc
     1561 ggcggcgtgg cgcgccgtgc tcgtccaccg caaagaacgg gccgtgctcc tgaccggtct
     1621 gcatggggta gcgcgtacgg ggcgcgccgg gctcgtcgca gccgtacatg cccccgcgtc
     1681 cggaaccgtt gtagaacacc cagtgggtct cggcgaaggc gggggcggcg ggggacggat
     1741 cgttggcgtg attgtgggtg gtcagatcga cgcccttgcc cgaggtccac aggttgacgt
     1801 agcacacctc gtcgccatct tcctcaagca ggtgaaatcc gggcgacatg tacgcctcga
     1861 cgccgtcgaa cgcgtgcgtg acgtggccga atttctcgtc gaatcgctgc cagcgaaaag
     1921 cgtcctcgag agggccgtgc agggtcagct cggacatgac ctcgacccgc tccggcgcgc
     1981 cgtcccctct cgtcatcacg gcaaccagcg cgccgtcgtg gcccgccgtc acttcttcac
     2041 tgagtaacag ggtgtcgcgg atctcgcgcg gacgggcgaa agcgccgcga tcggtgagcg
     2101 ctccggcgac caccttgagg aaaacgtcac ccggttcctg tgggatggcc aggcggtggc
     2161 cggccatcag gcggacgaac ctgatgatcc agcccgccac cgcccattcc ggcgatgcga
     2221 tggccggctg ggcgggcgtg ggcgcattca ggctgacgtc caccccttct ctgcgggtgc
     2281 tgcgggcgac tcccgcggtg gcaggcaggt cggataaaat gctcgagttt ccggtcatgt
     2341 cgatttcctt tctggccagt aacgtgcccg cagcggctgc ttgtagagct taccagtggg
     2401 cagcctcggc atctcgtcga tgaagtcgat ggagcggggc accttgaaac cggcgaggtg
     2461 ggcgcgcgca tggccgacga gctccgccgc cagggcgtcg ccggggctcg tgccgggcgc
     2521 cggctgcacc acggctttga cctcctcgcc gaactcctcg ttcggcaccc cgaacacggc
     2581 cacgtccgcc accttcgggt gaacgatgag cacgtcctcg atctcgcgcg ggtagacgtt
     2641 cacccctccg gagatgatca caaacgcctt gcggtcggtc aggtagaggt aaccctcgtc
     2701 gtccagatag ccgacgtcgc cgagggccgt ccagttgccg tggtcggggt gggtggcgtt
     2761 ggccgtcttg tcgggcgcgt tgtggtactc gaacggccgc tcgggctgct cgaagtagat
     2821 caggccttcc tggcccgggt cgagctcccg gccgtcgtcg tcacagatgt ggatgacgcc
     2881 gctgcgcgcc cgtcctaccg agccgggatg ggccagccag tcgtgggggt cgatcagggt
     2941 cgagccgttg cgctccgtgc ccgcgtagta ttcccagatg atcggtcccc accacgtcat
     3001 catctgctgc ttgatgggga ccgggcaggg tgccgcggcg tggacggcac aacggtggct
     3061 gctgaggtcg aatcccgttc gcgcctcgtc gtcgaggttc agcaaacgaa cgaacatggt
     3121 cggcacccac tggctgtggg taacccggta tcgctcgatg cagccgaggg cctccagcgg
     3181 atcgaatcgc tccatcatga tcaccgtgcc gccaaggctc agcgtccgca tgcagaatgc
     3241 gaacggcgcg gcgtggtaga ggggcgcagg tgacaggtag acgctctcct ggctgaaccc
     3301 gtaggggctc atctgctcca ccccgggtat gcctttcgcc ggcgattggc cggtcagggg
     3361 ccgcttgatg cccttgggcc ggcccgtggt gcccgagctg tagagcatgg aatcgcccac
     3421 ggtctcgtcg gcaaccgggg tgaaggggca ggcctcgatg gttttctcgt acgacgtcca
     3481 gcccggctct acgcctctgg ccatcagccg caccgggcac tcggcgatca gcggcagaag
     3541 cttgctgccc agaccgatct ccgcggagac gatgacggct ttcgccgacg agtccgtgac
     3601 gatgtaggct gcctcctcgg ctgtcaggta gcggttgacg caggtgatgt aaagacccga
     3661 gcggattgct gcccaggcga tctcgaagaa ctgcagatgg ttctccatga tcagcgcgag
     3721 gtgatcgccg gcgcctagcc cggcccggcg cagggcatga gcggcctggt tcgacctccg
     3781 gtccagatcg cgccacgtga gcgcatcccc gctgccggcg gccagcacgg ccacgtcttc
     3841 gcccttgatc tgcgcccagc tgcccggata catgctggcc tgcctccgct cgcccttcgc
     3901 catgatgcct gtcccgcggg cggcagggaa agggatcgca tggattttcc ggctgatgtt
     3961 aaattgcggt aagcagaggg aggaaagtca tgcacgtcgg attcgcaccc gtatttcaga
     4021 acctcggcaa gccggtaccc gaccatcagg tgtacgcgca agagctggcc atggcccgcg
     4081 ccgcggaacc catgggcttc gactccgttt ggtcggtgga gcaccacttc accgactaca
     4141 cgatggtgcc cgacgtcacg cagtttctga gctacatggc ggcctgcacg gagcgggtgc
     4201 agctcggctc catggtgatc gtgctgccct ggcacgaccc ggttcgcgtc gccgaggaaa
     4261 tcgccatgct cgatgccctc tccggcgggc gcatgatcct gggtatcggc cgggggctcg
     4321 gccgcgtcga gttcgatggc tttcgcgtgc ccatggacga gtcccgtgag cgtttcgtcg
     4381 aatcggcgac catgatcctc gaagggctgg agcgcggctt cgtagagctc gacggcaagc
     4441 acttccagca gcctcgccgc gacatccggc cggcgccgtt caggaccttc cgggggcgga
     4501 cgttcgccgc ggcggtgtcc ccggagtcca tgccggtgat ggcccggctt ggtgttgggc
     4561 ttcttgtgat cccgcagaaa ccatgggaat cggtcagaac ggatttcgag gtttaccacc
     4621 gggtctgggc agaaactcac ggcgcgcagt cgtctccgcc ggaccctatg tgcggcggct
     4681 tcttcttcgt cgacgagagc gccgatcggg ccgaggagat ggcctacaag tatatcggcc
     4741 gttattacca ctccgtcatg gaccattacg agcttcgcat cgggcacctg gaaggcacga
     4801 aaggttacga gttctacgag aagattcatg agcacatcga aacccgatcc gacgacggcg
     4861 cggcccggga cttcgtccgg ctgatgccgt tcggcacgcc cgagcaggtg atcgagaagg
     4921 tcgagttcat ccaccggatg atcggggcca aaggcgtgat gacccacttc gcctatggcg
     4981 ggatggacta cgccgaggcc aaccgcaaca tgcgtctgtt tgccgacagg gtcatgccgg
     5041 agctgcagcg gctgggcgag cagcgagggg ccttggcggt ggcgtgaagc cggattcagg
     5101 cgacccgcgg gctgcggccg cttccctgct cagcccgccg ctgcgtccat cagggcggct
     5161 acatgaaatc cgcgacgctt caggttggcg acgaactcgt cgtggaagac gtccggcacg
     5221 gagtttgcga ccgccgggct ggccgcaagg gcgtctgccc aggcgcccac cagcgggtaa
     5281 gactcgagca ggccggtggc cagccattgc tcgacaatga gaaaacgctg gaagaacggc
     5341 gcgtaggcgg catccaccag ggagagatcg ggcccgttga agaagggccc ttcgttgccc
     5401 cgctcggcgc gaatggcctc ctcgatcttg tcgaggcgct gtcgggcaac cgtcatgcgt
     5461 tcttcgtggc cctccggaga ctttgcgtag tacagggcgc tcaggccggc ggcgaaggtg
     5521 ggcacgaaat cggtccaggc acggttgcgg gcgcgcttta gcgcatcctc gggatgcagg
     5581 cgcgggggga ccacctcgtc gagatactcg gcgatggcgt tggactcgaa cagcgggacc
     5641 ccgtctacct gcagcacggg caccttgccg tggggggaaa tctccagaaa ccagtcgggc
     5701 ttgtcgcgca ggtcgatgta ggtcacgtcg aagtcgacgt ccttggcgcg catcacgatc
     5761 acggcgcgct ggacccaggg tcacgtgaag gagctgatga ggtgatatct cgcgggtgcg
     5821 ttcatgatgg ctgaagacta cgccgtcagg cggtgacctg acaagcgtac gcgaggttgc
     5881 agccgaccac tcgggcgttg gtctgagcga agttgccggt ataccatcgg tgccgaagcc
     5941 atgccatgga ttaccgttgc aggtatcgcg cgcgctcatc gtcggtggcg ggatcggcgg
     6001 actcacggcc gcgctctgcc tggcaaggca agggattgcc gtcagcgtgt tcgagcagac
     6061 ggccgatctc gccgaggtgg gcgcgggaat tcagctttcg cccaacgcct ctcgcgtgct
     6121 tcatcatctg ggtctcgagg gcgctctcgg cagcgtcgcc ttcctcccgc gcgccgtgga
     6181 gatgcgcgac tggcgaagcg gccgcctgct ggcctctagc cccatagacg aggctttccg
     6241 cgaccggttc gtctatccct actatcacgt gcaccgggcc gatctgctcg acgtcctgga
     6301 acgcgcgctg cggcgggcac cgggtatcga gctgcgcacc ggtgcgaggg tcgagggtat
     6361 cgagccgggc gactccgccg tcttcgtcga ggccggcggc gagcgtcatg gcggtgacct
     6421 gctgatcggt gcggacggta ttcactccac ggtccgcgag gcgttgttcg gcgccgaagc
     6481 gccgcggttc acgggcaacg tcgcctggcg gggcctggtg ccggcaggcg cggtcccctc
     6541 aggcttcgtc cggccggccg ccagcgcctg gtgggggccg cacaagcatt tcgtgcacta
     6601 ctacgtccgg agaggcgagc tgatcaactg tgtgggcgtc gtcgagaagc gcgggtggga
     6661 agtcgagtcc tggacggagc ctggagacct cagcgagctg caagctgact tcgccggatg
     6721 gcacgagacg gttcggcggc tgatcgacaa catggacccg caggcctgct tcaagtgggc
     6781 gctgttcgat cgtccgccga tgtcggcgtg gagcgatgga cgggtgacgc tcctgggtga
     6841 tgcctgtcac ccgacgctgc cgttcatggc ccagggcgcg gccatggcca tagaggatgc
     6901 ggcggtgctg gcgcggtgcc tggtggaagg cgacggggtt tccgggagcc tgcgccgcta
     6961 cgagtcgttg cgcagggcgc gaacggcccg ggtgcaggcc ggttccaggc ggaacgcgcg
     7021 gctcttccac tcgagcgggc cggctgccgg gctccgcaac ctgatcgtgg gccgcgcttc
     7081 gggtcgcgtc atggactggc tttacgggta cgacgcgctg gatcctgggg cgccatgatg
     7141 ggcgccgccg ggttccgtca cgcgctcagc gcaaaaccgt cgtagccgga ttccgccacc
     7201 tcccggcatt tttcggcgta gggcggaaaa cccagatagg gcatgaacac gcgaggcttg
     7261 cccggaatgt tcgcacccag ataccaggag ttgcagcgtg gatacaggct ggaatccccc
     7321 acctcgttca cgtgagcaac ccacgctgtc tgggcctcgg gcatcgcgtc gatgcgggac
     7381 atgccttcgt gctccatgta ggcgacgcag tcggcgatcc agtcgacgtg gtgttctatc
     7441 gtcgggagca tgttcgtcag cacggagggg cttccggggc cggtgacggt gaaaaggttc
     7501 ggaaaaccgg acacggccag gcccaggtag gtcttggggc cttccgacca tgcgtcctga
     7561 attgaacggc cgccgatccc gcgaatgtcc atcttcatca acgcccccgt catggcatcg
     7621 aagccggtgg cgaacacgat ggcgtcgaac tcgtactcct cgtcagccgt tcgcagtccc
     7681 ttgggcgtga tggcctcgat gggcgtgtcc cgcacgctca ccagggtgac gttttcgcgg
     7741 ttgaaggtgg cgtagtagtc cgtatccacg cacaggcgtt tgcaccccac gacgctggtc
     7801 gggcagagaa gctcggcggt gtccgggtct ttgacggtct cgcgtatctt gcctctgatg
     7861 aactcggcgg cggtctcgtt ggcgtcggcg tcgaacacga ggtcggcgaa ggcggccagg
     7921 aagggcaacc cgccccgttc ccagcgcgcc tcatactcgc gctcgcgctg ctcggcggat
     7981 acgtccaggg cgttggactc gttgtagcgg aaatccgcgt gaaaggccgt ctgccgattc
     8041 gcatcccgga actcggggta tcgggccttg atccgggcga ccgctcccgg atccagggcc
     8101 cgattgtgcg cgggcacgga gaagttgggg gagcgctgga agacggtgag ctgctccgcc
     8161 tgctcggcga tgagcgggat gctctggatg gccgacgagc cggtaccgat cacggcgaca
     8221 cgcttgccgg tgaaatccac gccttcgtgg ggccattgcc cggtgtggta ccagtcgcct
     8281 tgaaagctgt cgatgccatc gaattcgggc aggttcgtgg aggaaaggca gccggttgcc
     8341 attaccaggt actttgcttc gtgctcgccg ccatcgctcg tcgtgacggt ccaccggttt
     8401 gtttcttcgc tgaaccgggc tcgctccacc cgggtgttga gctgtatgtc cccgcgcaga
     8461 tcgaagcgtt cggcaacgtg gtttatgtag ccgaggattt ccggttgggt agcgtaacgc
     8521 tccgaccact cccattcccg ggcaagctcg tcggagaact ggtaggagta ctccatgctc
     8581 tcgatgtcgc agcgcgcgcc cgggtatcgg ttccagaacc aggttccgcc gatgtcgctg
     8641 ccggcctcga agacgcgggt tttcaggccc aggccgcgca ggcggtggag catgtagagc
     8701 ccggcgaagc cggcccccac cacgatggcg tcgagtggct ttgtgttcat ttcctttctt
     8761 ccccctgctg ttgcctcgca gcgctgcccg gatgccgtga acgcggccgt ccggcaggga
     8821 catcgcggcg aatgtcctct tggtaaatct taacccatga cgtgcaagcc ttttacaccc
     8881 gggccgggtc gatcgtgttt gaatgggcgc actgggaaat gggagacgca agggatgcag
     8941 ccgcagtcgc cgcgccacga ttcggacgag aatgatatcg gggccgttcg ccaatcccgg
     9001 gcgcgagcca aggtgacgct cgatgccggg tgaatcctgt cgggtcggat accgccacgc
     9061 catggacgcc gtgcctggtt ccgtctggcg gacgctggcg gatttcggat cgcttctgag
     9121 ctgggtagcc gggggcgacg agggccgtat cgagctgtcc ggtgagggca tcggcatgac
     9181 gcgggacttg tggctcccca gcgtaggtcg ggtgcagcat cgactcgatg tcctcgacga
     9241 ggccggccgc tgcctcacct acagcctcgc cgagggtcgt ccgctgggca tgcggagcta
     9301 ctcggtgacc gtgagcgtgg aagcggccgg atccggctgc acgctggcgt ggcagggtgc
     9361 gttcgttccg gagcctggcg ccgattcgga tgccatggcc gaaaacctgc gccgagccta
     9421 cgtcgacatg tctcggcggc tcgatgtgct gctgcgagca gcgtgacccg tcgcctgcaa
     9481 gcctgcatca gcggcaatgg caaagacgag ggcgataagt tcgatgccgt gatcaggtcg
     9541 ctcgagggct gcgccaccag gatatgagca ggcagacgat catggccccg gcagaccccc
     9601 accacagccc gctcgggccg aggctctgat acaagacccc gccgcctgcg gcggtcacgg
     9661 cgccgacggc cattcctgtg gcgccgaaca ggccctggcc cgctgccagg gccccctcac
     9721 cgcttgcccg tccgactgcg agctggctcg ccggcatggt gtaggcgtcg gcgatggcgt
     9781 gaatgcccag cggaacgcat agcagccaca gcgagtccag aaacccgtag agaaccatgc
     9841 atacggttgc gaccgccatg ctgaccgagg ccacccggag aggcccccgg tgctgggcca
     9901 gggagccggc acggggcgcg atgaagatca tcgggatccc gaagagactc atggtgagcc
     9961 cgatgaacat ctggctggcc cccagatcgg cgagaaaaat ggcccagacc gcttcgaaga
    10021 cgccgatggt gatccagaac gcgatgccga gagcaagaca tgcctgcatg taaggattgc
    10081 gcaacagcgt gccgatgacg ggacccgttg tcggtcggct ggccccggga atgctcacgc
    10141 gccacaccat gggcgcgagc agcgccagga cgcccgtcat cacgatgaag gtggacgcca
    10201 ggccagccca gcggtcgagc agcgcggcga gcacggggcc gagaaggaag ccagccgtct
    10261 cgtaggcggt caaacgtccc atggcgtggc cggcattggc ggggtctgcg gtgagtgcca
    10321 gacgacgtac ggcggggcgg acgcagccgg cgccgaaccc cagcagggcg cgcgcggcaa
    10381 tccacgcagc gagggtgtcg gcgacggcca tccacagggt gctcagcgcc gagatcgcga
    10441 gacctgcccg cagcatgatc gccccgtaac cgcgatccgc gtaccgcgca agccacagct
    10501 gggcgaagaa accggccgcg aacgcagagc ctgcgatcag cccgatgccc gtaggcgtga
    10561 agccgaacgc gtcgcggatt tcggccagca gggaaaacac cgccccgtag cccaccatca
    10621 ggacggcggt cagaaggaaa agggctttga gcgtggtatc ggctgccttg tcaggtctgg
    10681 taggtgaacg gtcgatcgtc gtggtaacgt ttggtcggtg atggtccatc gaaccgcaat
    10741 ctagggcgga aagcaaagat catgcgagga ggggaagggt gaacgacaac gcgatcgcgc
    10801 tggtgaccgg aggggcaacc ggtatcggcg ccgaggtgtg cagacagctc gccgcggcgg
    10861 gagcaagggt ggccatctgc gacgtcaacg aggcagacgg cgcgccgctg gccgagtccc
    10921 tgggcggcga gtttctgttc tgcgacgtgg cggacctggc gagcgtggag ggagcggtcg
    10981 ccggctgctg tgcccggctt ggggtgcccg actacgtaca cctgaatgcc ggcgtcatga
    11041 ctgtgggccg ctacgatgcc tacctcgcgc tcgaggacgt cagcgtggag cagtaccgcc
    11101 ggatcatcgg cgtcaacgtg gacggcgtct tcaacggtat caaggcgctt ctgcctccca
    11161 tgaggcagaa gggcggagcc atcacggtga cggcctccat tgccggcttc gccccgatgg
    11221 ccatcgatcc gctctacggc gccaccaagg ccgccctgat cagcctcgtg cgcagcgtcg
    11281 cggcagccaa cgaaggcggc agcgtgcgta tcaacgccat ctgcccgggg gtggtggaca
    11341 ccgccatagt gcccgatgcg atgtccggca cgataccgat gatgccaccg tccgccatgg
    11401 cggctgagat actggacctc ctcagcgatg gcccctccgg cgagatccgc gtcaagctca
    11461 acgaggagac gcccgccttc cacgtcgagc cgagcacctt gggtcgctga acgggcaggc
    11521 catcatgaat ttcgacgatt accatgccat cgtccgtcgc cgctacgagt acgcgctggg
    11581 gatcgacacg cgggactggg tgctctatcg cagcatcttc accgacgaga tcaccatgga
    11641 cttctcttcc tacaatggcc gacccgcatc cggcatggcg gccgacgact gggtggccgg
    11701 ctgtaaggtt ctgtttgccg ggctcgatgc cacccagcac gtgatgtcca atccgatggt
    11761 cgacgtcgac ggggataatg cccggctgcg catgtacatg aaggccgagc actttctgcg
    11821 caacgatcag ggcggcatgg atttcaccat cggcggctac tacgacgacc ggctggtcag
    11881 gacggatgag ggctggaaga tgcaggcggt gacgttgaac gtcttctgga gccgcggcaa
    11941 tcggcagatc atggcgctcg cggcggagat tggaaagggc aagctcgaag aagcgtcgtc
    12001 gggttcgtga cagcgcgtta gggagtacgg ccgatgaacg atccggagtc cattgcgaac
    12061 ctcgaggcgc gcatggcctg ggagcaggaa cgggcggttc cgcccgaggg ctttccggcg
    12121 ttgccgcctc tgccgcccgg ccgctacacc agcccggcat ttttcgatct cgagcgtcgt
    12181 cacctgtggt caaaggtctg gctctacgcg gcccacgaca gccagctgcc ggagcccggc
    12241 agctttctcc tgctggacat ccccgatgcg ccgatctttc tgattcgtgg ccgcgatgca
    12301 agcgttcgcg cgttttacaa cgtctgctcg catcgcggag cgccgttggt gcgggaggcc
    12361 tccggtcagg tggcccggct tcgctgcacc taccaccagt ggacgtacga cacggacgga
    12421 cgactgatcg ccgtcatgga cgagcgcgac ttcgcggagc ccttcgatcg atcctgcctc
    12481 ggcctgaagg aagttcgctg cgagcagtgg ggtcggtgga tattcatcaa cgaggacccc
    12541 gaagcacagc cgctgctgga atggctcgac atgatcgccg ccgagttcga gcagttcgac
    12601 atcgagcgcc tgcgcccggc ggcgatcaag agctacgagc tcgagtgcaa ctggaagctg
    12661 accatggatg cgttcctgga ggtctaccac atacagggca tccacccgaa gaccgtgggg
    12721 ccatcgctcg accaccgggg cgccgtcatg gggctgcttc ccaacgggca cacgcgcatg
    12781 acttgcccga cccggatgtc ccgcaccgag accgccgaca cgcgcggata caccgattca
    12841 gcaggcgagg cgatgcctgc cattccggct ggcgagatcg ctcgcaacta tcacgtctcc
    12901 cacaacgtct ttccgaatct catcacgccc gttggcgaca cggccaggca gtttctgatg
    12961 ttctggcccc tttcgctcac gcggacccgg gtggacgtgg tccatttcgg cctggactgg
    13021 ggtgagggcg agcgtccctc gggatgggat ggcctgctcg ccttctggga cgtggtgatg
    13081 gccgaggatc tgcagtttct ggagtggcag cagaaggccg tgctctcgcc aggatttcag
    13141 ggctaccgcc tgagctacat ggaacgccgg atctactacg cccacgagtc catcgaccgg
    13201 atcatcggcg ttgagaacat tcccgaggcg ctgcgcgccg tgcccatgct ggatgcctac
    13261 cgcgagcgcg cggaagaccg ctgcgcggat cagccgtcga gcccgtcctc ccgttaaccc
    13321 cggggttccc gtgcccgagg ggcccggtga tccctaccgt gccgtgcgcc tggcgttacg
    13381 ccgctcgatc cgctcccgca gccgggcgtg ccgaacggga gtgagcgggt atgaccagat
    13441 gagcttcagc gtcagtccgt agaagactat gggccccagg cagtaggcga gccgcagggc
    13501 cagcaccggg ccggtcaacg tggcgcggtc cacgggtcgc ctgttgagaa tgtctcccgt
    13561 gaaggcgctg agcgcgtctg tccagatctg cgcggccacc tggagcacgc ccacgccgtg
    13621 gtcggcccgg atctccacga tcatcggcac ggtggcggcc accatcagcc ccaccagcac
    13681 gtagaactcc cgggcagcgg tcacccggct gcgctgatgg caggcgggcg accatcagga
    13741 tggtgcccac cagcgccaga gagataccga gccctcccaa gtaatgggcg gggatccaga
    13801 tcgccagcgg gtatccgatc acggcaagcg gcaggccgag gcagccgtga atccagatgg
    13861 tgggtctggg caggggaaga actggagacg acgcgtcgtc tgccgcagcg ctcatggctt
    13921 tctcgctcgt cgctccgtga gcagggtatt atccacggct ttcgcgcagt ggatggaagc
    13981 cggaatgaag gtcagcatcg gcatcggcgg ggccgcctct gggggccgac gtgatttcga
    14041 agatcaggtc gcctacgtcg tcgaggcgga agccctcggc gtggataccg tctggacggc
    14101 ggaagcatgg ggccaggacg ccatcagccc tcttgcctac ctggcggcga aaacgcaacg
    14161 gatacggctc gggacgggga tcatgcagat cagtgcccgc acgccttcca tgaccgccat
    14221 gacggcgctc accatggccg ccatctccaa cgaccgtttc atcctgggcc tgggtgccag
    14281 cggtccccag gtggtcgaag ggctgcaggg acaaccgttc aaagccccgt tgacgcgcat
    14341 gcgcgagacg gtggacatca tcaagctggc ttttgccggg gagaagctgg cttacgacgg
    14401 caagtatcac cgactccccc tgcccggcgg cg
//
LOCUS       DBNHQR010000368         2121 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_98051, whole genome shotgun sequence.
ACCESSION   DBNHQR010000368 DBNHQR010000000
VERSION     DBNHQR010000368.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_98051"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="AC16VR_08320"
     CDS             <1..355
                     /locus_tag="AC16VR_08320"
                     /inference="COORDINATES: protein motif:HMM:NF013237.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HIR6011200.1"
                     /translation="GREDVERVSYPGLPGSPYRDRVARYLPRGAGAIFCFDLPGGREA
                     GQRFIEQLTLFSHLANVGDAKSLVIHPATTTHRQLSDEELAAAGIGPGTIRVSIGIED
                     ADDIIWDLEQGLAGI"
     gene            357..842
                     /locus_tag="AC16VR_08325"
     CDS             357..842
                     /locus_tag="AC16VR_08325"
                     /inference="COORDINATES: protein motif:HMM:NF024773.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIR6011201.1"
                     /translation="MTEHDMERYQDPHVIQGALNQRRIAVVGLSGNPLRASNFVGYYM
                     KRHGYEVVPVNPREEEILGLKSYPDLAAVPEPVGVVDVFRDPSAVPDIARAAVAAGAK
                     YLWLQFGVISFEGAEIAEAGGMQVIMDRCIKIEHARYRGRMHWLGFNTNQISAQRRRA
                     D"
     gene            973..1485
                     /locus_tag="AC16VR_08330"
     CDS             973..1485
                     /locus_tag="AC16VR_08330"
                     /inference="COORDINATES: protein motif:HMM:NF016408.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactofilin family protein"
                     /protein_id="HIR6011202.1"
                     /translation="MFRKVKGGTRMAPAMGERDEIRGELTHFSPADAANEATIGATIV
                     IKGEITGDENLVIEGTLDGTANLSDRNLTIAQTGRILGNVSANSITVEGEIQGNLTAT
                     ERVFVAKTGKVLGDIMAPRVSVEGGAKFTGRIDMDTKGGRVVRPSGPKRSETLTRPAA
                     ETAPAAENKA"
     gene            complement(1549..>2121)
                     /locus_tag="AC16VR_08335"
     CDS             complement(1549..>2121)
                     /locus_tag="AC16VR_08335"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6011203.1"
                     /translation="WAIVVAHFCNNWSLYVLLSWLPTFVNKGLGVDYSAVGWFTMIPH
                     LTAFVFLNVAGGVADRLIHRGMDVGRVRKLMQTIGFGGIAAALLVVGEVPSAWMAIGV
                     MSVGNALGAFCTGGFAVNHMDIAPRHAGTLMGITNTAGTLPGIVGVFVSGVILELTGS
                     WALVFQVAAGVNLFGLVFYLTFASGRRLID"
BASE COUNT          430 a          689 c          706 g          296 t
ORIGIN      
        1 cggtcgcgag gacgtcgagc gggttagcta ccccggcctg cccggaagcc cctatcgaga
       61 ccgggtcgcc cgctacctgc cgcgcggcgc cggagccatc ttctgcttcg acctgccggg
      121 cgggcgcgag gcgggccagc ggttcatcga gcagctcacc ttgttctccc atctggcgaa
      181 cgtgggcgac gcgaagagcc tggtcatcca cccggccacg accacccacc gccagctgag
      241 cgacgaggaa ctggcggccg ccggcatcgg tccgggcacc atacgcgttt ccatcggcat
      301 cgaggatgcc gacgacatca tctgggatct cgagcaggga ctggcgggca tctgacatga
      361 cggagcacga catggaacgg tatcaggacc cccacgtgat ccagggcgcg ctcaaccagc
      421 gcaggattgc cgtggtgggg ctgtcgggca accctctgcg ggcgagcaac ttcgtgggct
      481 actacatgaa acggcacggc tacgaggtgg ttcccgtcaa tccccgggag gaagagatcc
      541 tcgggctgaa aagctatcca gatcttgcag ccgtgccgga gcccgtgggc gtggtggacg
      601 tcttccgcga cccgagcgcg gtgccggaca ttgcgcgcgc cgcggtggcg gccggcgcga
      661 agtacctgtg gctgcagttc ggcgtgatca gcttcgaggg cgccgagatc gccgaagctg
      721 gcggcatgca ggtgatcatg gaccgctgca tcaagatcga gcacgcacgc tatcgggggc
      781 gcatgcactg gctcggcttc aataccaacc agatctccgc acagcgccgc cgggcggact
      841 gaaatccgca gcgccgacct ggatatagct acttgtcaat tcggccggtg ctcagttaat
      901 ctgcacggtg aaaggagccc caatcgaaca aaagaaaggg ggcatgggga attgggacac
      961 gaggccgaag gcatgtttag aaaggtcaaa gggggcaccc gcatggcacc cgccatgggg
     1021 gaacgggacg agattcgcgg agagctaacg cacttcagcc ctgcagacgc agcaaacgag
     1081 gccacgatcg gagcaacgat cgtgatcaaa ggagagatca cgggcgacga gaacctggtc
     1141 atcgagggga ccctggacgg taccgccaac ctctcggacc gcaatctcac catcgcccag
     1201 acgggtcgga ttctcggcaa cgtcagcgcc aactcgatta ccgtcgaggg ggaaatccag
     1261 ggcaacctga ccgccaccga gcgggtgttc gtcgccaaga ccggtaaggt cctcggcgac
     1321 atcatggcgc ccagggtgag cgtcgagggt ggcgccaagt tcaccggccg gatcgacatg
     1381 gacaccaagg gtggccgggt cgtcaggccg tcgggtccga agcgctcgga gaccctgacc
     1441 cgcccggccg ccgagaccgc cccggccgcc gaaaacaagg cctgagaacg ccgcagccgc
     1501 ggatgcgggg cacctcgcga cgaggtcgtc ccgcgctcgc gcgcctgatc aatcgatcag
     1561 gcgcctgccg ctggcgaacg tgagatagaa cacgaggccg aacaggttga ccccggcggc
     1621 cacctgaaac accagggccc aggatccggt cagctcgagg atcacgccgc tgacaaacac
     1681 gcccacgatg cccggcaacg ttcccgccgt gttggtgatc cccatgagcg tacccgcatg
     1741 gcgcggcgcg atatccatgt ggttgacggc aaagccgccg gtgcagaacg cgcccagcgc
     1801 gttgccgacg gacatgacgc cgatggccat ccatgcggac ggcacctcgc cgacgacgag
     1861 cagcgcggcc gctatgccgc cgaagccgat ggtctgcatc agcttgcgga cgcggccaac
     1921 gtccattccg cgatggatga gccggtcggc aacgccgccg gccacgttga ggaagacgaa
     1981 ggccgtcagg tgcggaatca tcgtgaacca tcccaccgcg gagtagtcca cgcccaggcc
     2041 cttgttgacg aacgtcggca accagctcag cagcacgtag agtgaccagt tgttgcagaa
     2101 gtgggcgacc acgatggccc a
//
LOCUS       DBNHQR010000369         3318 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1554718, whole genome shotgun sequence.
ACCESSION   DBNHQR010000369 DBNHQR010000000
VERSION     DBNHQR010000369.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1554718"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="AC16VR_08340"
     CDS             <1..1081
                     /locus_tag="AC16VR_08340"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6011204.1"
                     /translation="PAQTLIARQLLDKHIEPFDTPRVNAAMGGVLVLRPTPETWRAHR
                     QELTDPWVGPGAGRNEEMIRRTADELIDRWIDRGTVDFVSEFAQPLPQMVMANILGWP
                     LEDLKLLKYYGDGTVKPFVFGKGHRSVLPEEEVKSQFEVLEEFKTYTIALIEEKRRNP
                     ADDMITFLTEVEYSPVGRKLNDNEINSVVYNMVIGGLETTQYALSEQVQLLIERDGVW
                     DEIKADRSKVRGFTEDAMRLRAPTQGLSTRTTSRDEVFQGVHVPKGSLLHLRWAAGNI
                     DPDEWECPMDLKLDRRAGTRHLTFSQGARVCPGASLSRLEQTVSWNALLDKVDRFAYG
                     EDNNFMHQPGIMLGLEYLNLTFGKA"
     gene            complement(1105..3234)
                     /locus_tag="AC16VR_08345"
     CDS             complement(1105..3234)
                     /locus_tag="AC16VR_08345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-containing oxidoreductase family
                     protein"
                     /protein_id="HIR6011205.1"
                     /translation="METAKSYCRYCHAYCAIDVDVEDGRPVAIRGDAKDPVYGGYTCI
                     KGRQLPELYDAPDRLHTALKKMPDGRFEEIPTARALDEIAARIERIIERDGPRAVASY
                     CGTYAFMESAALALARSFHQGIGSESFYTSVTIDQPGRPIAMSRFGTWGGGTHSFADA
                     DVVMSIGNNAVVSSFSVFGGIPPFNPYRRMNDAIKDGKKLIVIDPRRTEVARKAHLFL
                     QVRPGEDPTLLAGIIRVILDEDLHDQPFCDQYVDGLDAIKAAVDPFTLEYVARRAAVP
                     AEQIAEAARTFAAGPKGVAVTGTGPNMAERAPLTEHLVLALNTICGRVNRAGERLPNP
                     GILSPLTAVKAEVIPPAPAWGHGPRNRVRGLGQIFGEMPTAALSDEILEPGEGQVRAL
                     LSIGGNPIVAWPDQIKTKRALDALDLLVCVDIRLSATAQLADYVIPGKICLEREDMPV
                     LTDNWYDVPYGHYTEAVCQPNGDLREEWEFYWELAHRLNSDLPLPGGSLPTQEKPGKQ
                     TVLEHMFPNPKVPIADIRAQDGGHVFEGVQVMVEPGQGDARLQLTPGGVADELAEVRA
                     ETLQENGAPGKDGDYSHLLISRRLKHVYNSSGQQLPEIRKKGTTNPAYMNPEDVAALG
                     IGDGDVVQISSRHGQILGVAAAAPDVKRGVISMAHAWGGLPGNDGEVREIGASTNRLL
                     SNDKEYDPITGMARQSAIPVNVRPVAL"
BASE COUNT          517 a         1088 c         1143 g          570 t
ORIGIN      
        1 cccggcccag acgctgatcg cccggcagct gctcgacaag catatcgagc ccttcgacac
       61 tccccgggtc aacgcggcca tggggggcgt gctggtgctg cggcccacgc cggagacatg
      121 gcgggcccac cgtcaggagc tgaccgatcc ctgggtgggg ccgggagccg ggcgcaacga
      181 ggagatgatc cggcgcacgg cggacgagct gatcgatcgc tggatcgacc gtggcacggt
      241 ggatttcgtt tccgagttcg cgcagccgct gccgcagatg gtcatggcga acatcctggg
      301 ctggcctctg gaggatctga agctcctgaa gtactacggc gacggtaccg tgaagccttt
      361 cgtcttcggc aagggccacc gcagcgtcct gcccgaggag gaagtgaagt cccagttcga
      421 ggtgctcgag gagttcaaga cctacaccat cgcgctcatc gaggaaaagc gccgcaaccc
      481 ggccgacgac atgatcacct tcctcaccga ggtggagtac tcgccggttg gccgcaagct
      541 caacgacaac gagatcaaca gcgtcgtcta caacatggtg atcggcggcc tggaaacgac
      601 ccagtacgcg ctgtccgagc aggtgcagct gctcatcgag cgtgacggcg tctgggacga
      661 gatcaaagcc gatcgcagca aggtcagggg attcaccgag gacgccatgc gcctgcgcgc
      721 gcccacacag ggcctttcga cccgcaccac cagccgggac gaggtgttcc agggcgtgca
      781 cgtgccgaag ggttcgctgc tgcatctgcg ctgggcggcg ggcaacatcg atccggacga
      841 gtgggaatgc ccgatggacc tcaagctgga tcgcagggcc ggcacccgac acctgacttt
      901 ctcccagggc gcgcgggtgt gcccgggggc gagcctgtcg cgcctggagc agacggtttc
      961 gtggaacgct ctgctcgata aggtcgaccg cttcgcttac ggcgaggaca acaacttcat
     1021 gcaccagccg ggcatcatgc tcggcctgga atatctgaac ctgacgttcg gcaaggcctg
     1081 acggccggcg ccagcgaccg ggcgctagag cgccaccggc cgcacgttca cgggaattgc
     1141 gctttgccgc gccatccccg tgatcgggtc gtattcctta tcgttgctga gcagccggtt
     1201 cgtgctggcc ccgatttccc gtacctcacc gtcgttcccg ggcaggccgc cccaggcatg
     1261 ggccatggag atgacgccgc gcttgacgtc cggggctgcc gccgcgacac cgagaatctg
     1321 gccgtggcgg ctgctgatct ggacgacgtc accgtcaccg atccccagcg ctgccacgtc
     1381 ctcggggttc atgtaggcgg ggttggtggt gcccttcttg cggatctccg gcagctgctg
     1441 gccggaggag ttgtacacat gcttcagtcg ccggctgatc agcagatgtg agtagtctcc
     1501 gtccttgccc ggcgcgccgt tctcctgcag cgtctctgcg cgcacctcgg ccagctcatc
     1561 cgcgacacca cccggtgtga gctgcaggcg ggcgtcgcct tggccgggtt ccaccatgac
     1621 ctggacccct tcgaaaacgt ggccgccgtc ctgggcgcgg atgtccgcga tgggtacctt
     1681 ggggttggga aacatgtgct ccagcaccgt ctgcttgccg ggcttctcct gcgtgggcag
     1741 agacccgccg gggagcggca ggtcggaatt cagccggtgg gccagctccc agtagaactc
     1801 ccactcttcg cgcaggtcgc cgttgggctg gcagaccgcc tcggtgtagt gcccgtaggg
     1861 cacgtcgtac cagttgtccg tgagcacggg catgtcctcg cgctccaggc agatcttgcc
     1921 gggaatcacg tagtcggcga gctgggccgt ggcggagaga cggatgtcga cgcagaccag
     1981 caggtccagc gcgtccagag cccgcttggt tttgatctga tccggccacg ccacgatggg
     2041 gttgccgccg atggagagca gggcgcgtac ctgcccctcg cccggctcga gaatctcgtc
     2101 gctcagcgct gcggtcggca tctcgccgaa gatctggccc agcccgcgca cccggttgcg
     2161 gggcccgtgt ccccaggccg gcgcgggcgg gatgacctcg gctttcacgg ccgtcagcgg
     2221 ggacaggatg cccgggttcg gtagccgctc gcccgcccgg ttgacccggc cgcagatggt
     2281 gttgagcgcc agcaccaggt gttcggtcag cggcgcgcgc tcggccatgt tggggccggt
     2341 gcccgtgacc gccaccccct tgggcccggc ggcgaatgtg cgggcggctt cggcgatctg
     2401 ctcggccggt acggccgcgc gtctggcaac gtactccagg gtgaacgggt cgacggcggc
     2461 cttgatggca tccaggccgt caacgtactg gtcgcagaaa ggctgatcgt gcagatcctc
     2521 gtcgagaatc acgcggatga tgcccgccag cagcgtcggg tcctcgccgg gccgaacctg
     2581 caggaacagg tgcgccttgc gggccacctc ggtgcgccgg gggtcgatta cgatcagctt
     2641 cttgccgtcc ttgatggcgt cgttcatgcg ccggtagggg ttgaaaggcg ggatgccccc
     2701 gaagacggaa aacgaggaaa ccacggcgtt gttgccgatg ctcatcacca cgtcggcatc
     2761 ggcgaagctg tgggtgccgc cgccccaggt gccgaagcgg gacatggcga tgggccggcc
     2821 gggctggtcg atggtcaccg aggtgtagaa ggactccgag ccgatcccct ggtggaagct
     2881 gcgcgccagc gccagtgcgg ccgactccat gaaggcgtag gttccgcagt agctggccac
     2941 ggctctcggc ccgtcgcgct cgatgatccg ctcgatgcgc gcggcgatct cgtccagggc
     3001 ccgggccgtg ggaatctcct cgaagcgccc gtcgggcatc ttcttcaagg cggtgtgcag
     3061 acggtcgggg gcgtcgtaga gctcgggcag ctggcgcccc ttgatgcacg tgtacccgcc
     3121 gtagaccgga tcctttgcgt cgccgcgtat ggcgacgggc cgcccgtcct ccacgtccac
     3181 gtcgatggcg cagtaagcgt ggcaatagcg gcagtagctc ttggcggttt ccatccgcgt
     3241 cctcccccga attcgccgaa tcaagctttg cgcacggcga ggtgcgattc aagtcccggc
     3301 gagttgccgg tcgctggc
//
LOCUS       DBNHQR010000370         2758 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1744432, whole genome shotgun sequence.
ACCESSION   DBNHQR010000370 DBNHQR010000000
VERSION     DBNHQR010000370.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1744432"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1532)
                     /gene="carB"
                     /locus_tag="AC16VR_08350"
     CDS             complement(<1..1532)
                     /gene="carB"
                     /locus_tag="AC16VR_08350"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003095207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HIR6011206.1"
                     /translation="MPKRTDIDSILILGAGPIVIGQACEFDYSGAQACKALREEGFRV
                     ILVNSNPATIMTDPAMADATYVEPVEWATVARIIEQERPGALLPTMGGQTALNCALDL
                     VREGVLERFGVELIGASQDAIDKAEDRERFDKAMKRIGLETPRSAIAHSMEEAVQVQA
                     QLGFPCIIRPSFTLGGSGGGIAYNAEEFAEICARGLDLSPTNELLIDESLLGWKEFEM
                     EVVRDCVDNCIIVCSIENLDPMGVHTGDSITVAPAQTLTDKEYQLLRNASIAVLREIG
                     VDTGGSNVQFAIHPETGRLVVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDE
                     LDNDITGVTPASFEPSIDYVVTKVPRFTFEKFDQADSRLTTQMKSVGEVMAIGRSFQE
                     SLQKALRGLETGMTGLDPLLEIGGEEAVARLRRELATPGAERIWYVADAFRFGLSLEE
                     VFELSAIDPWFLAEIEDLIAAESQLAGKRLDQIGADALYRLKRQGFSDARLGTLLGAP
                     EQ"
     gene            complement(1525..2652)
                     /gene="carA"
                     /locus_tag="AC16VR_08355"
     CDS             complement(1525..2652)
                     /gene="carA"
                     /locus_tag="AC16VR_08355"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008171520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - L-glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HIR6011207.1"
                     /translation="MVRALLALEDGSVFKGLSIGVEGTSVGEVVFNTAMTGYQEILTD
                     PSYARQIITFTYPQIGNTGINPEDVESDRIWASGMVIRQTPKRASNWRMRQRLEDYLV
                     AEKVVAIADVDTRRLTRLIREKGAMAGCIAAGEAVSEAEAVALARDFPGLAGMDLAQV
                     VTRAEAGSWTQTDWSLESGYGEQTDARFHVVAYDFGVKRNILRLLAARGCRLTVVPAT
                     TPASSVLALEPDGVFLSNGPGDPEPCGYAIEAIGELLDRQIPIFGICLGHQLLGLASG
                     ATTVKMPHGHHGANHPVKDLRDGRVVITSQNHGFAVDADSLPDCLTATHVSLFDGTLQ
                     GIRRSDRPAFGFQGHPEASPGPQDCQYLFDEFIELMESRHA"
BASE COUNT          493 a          910 c          871 g          484 t
ORIGIN      
        1 tgctgctccg gagcccccag cagcgttccc agccgcgcgt ccgaaaaccc ctggcgtttg
       61 agccgataca gcgcgtccgc gccgatctga tcgagccgct tgcccgcaag ctggctttcc
      121 gcggcgatga ggtcctcgat ctcggcgaga aaccagggat cgatggccga cagctcgaac
      181 acctcctcca gcgagagccc gaaacggaat gcgtcggcga cgtaccagat ccgttctgca
      241 cccggggtgg ccagctcgcg ccgcaaccgg gcgacggctt cctcaccgcc gatttcgagg
      301 agcgggtcga gcccggtcat cccggtctcc aaaccccgca gcgccttctg cagggactcc
      361 tgaaagctgc ggccgatggc catgacctca ccgacggact tcatctgcgt cgtgagccgt
      421 gagtcagcct ggtcgaactt ctcgaaggtg aaacggggca ccttggtgac gacgtaatcg
      481 atgcttggct cgaaagaggc gggggtcacg cccgtgatgt cattgtcgag ctcgtccagc
      541 gtgtagccca ctgccagctt cgcggcgatc ttggcgatgg ggaagccggt ggccttggaa
      601 gccagcgccg acgaccgcga aaccctcggg ttcatctcga tcaccaccaa ccgaccggtt
      661 tccgggtgga tggcgaactg cacgttggag ccgccggtat ccaccccgat ttcccgcaga
      721 acggcgatgg aggcgttgcg cagcagctga tattccttgt ccgtgagcgt ctgcgccgga
      781 gccaccgtaa tgctgtcccc ggtgtgcacg cccatggggt ccagattttc gattgagcag
      841 acgatgatgc agttgtccac gcaatcgcgg accacctcca tttcgaactc cttccagccc
      901 agcaggctct cgtcgatgag cagctcgttg gtgggcgaca ggtcgagccc ccgggcgcag
      961 atctccgcga actcctccgc gttgtaggcg atgccgccgc cgctgccgcc caaagtgaac
     1021 gacgggcgga tgatgcacgg aaaaccgagc tgggcctgga cctgaacggc ttcctccatg
     1081 ctgtgcgcga tggcagatcg gggtgtctcc aggccaatgc gcttcatggc cttgtcgaac
     1141 ctttcccgat cctcggcctt gtcgatggcg tcctggctgg caccgatcag ctcgacgccg
     1201 aaacgctcga gcacgccttc cctgaccagg tccagcgcgc aattgagcgc cgtctgcccg
     1261 cccatggtcg gcagcagcgc acccggcctc tcctgctcga tgatgcgcgc cacggtggcc
     1321 cactccaccg gctcgacgta cgtcgcgtcg gccatggcgg gatccgtcat gatggtggcg
     1381 ggattggagt tgacgagaat cacccggaag ccttcctcgc gcagcgcttt gcaggcctga
     1441 gcgccggagt agtcgaactc gcaggcctgg ccgatcacga tgggccccgc gccgagaatg
     1501 aggatggaat cgatatcggt acgcttaggc atggcgcgat tccatgagct cgatgaactc
     1561 gtcgaacaga tactggcaat cctggggccc ggggcttgcc tccggatgcc cctggaaacc
     1621 gaaggccggc cggtcggagc gacgaatgcc ctgcagcgtc ccgtcgaata gagagacatg
     1681 ggtggccgtg aggcagtcgg gcaggctgtc ggcatccacc gcgaaaccgt gattctggct
     1741 ggtgatgacg acgcggccat cgcggaggtc cttgaccggg tgattggccc cgtggtgacc
     1801 gtggggcatc ttgacggtag tggccccgct ggccaggccc agcagctgat gacccaggca
     1861 gataccgaat atgggtatct ggcgatcgag cagctcgccg atggcctcga tggcgtaacc
     1921 gcacggctcc ggatcgccgg gaccgttgga tagaaagacc ccgtccggct ccagggcaag
     1981 aacgctggag gccggggtgg tggccggcac gacggtcagg cggcagccgc gcgccgccag
     2041 caggcggaga atgttccgct tgacgccgaa gtcgtaggcg acgacgtgaa aacgcgcgtc
     2101 cgtctgctca ccgtaaccgc tttcgagcga ccagtcggtc tgagtccagg aaccggcctc
     2161 ggccctggtc acgacctgcg ccaggtccat gccagcaagc ccgggaaagt ctcgggccag
     2221 cgccacggcc tcggcttcgc tgaccgcctc gccggccgct atgcatcccg ccatggcgcc
     2281 cttctcccgg atgagccggg tcagcctgcg ggtgtccacg tccgcgatgg cgaccacctt
     2341 ctccgcgacc aggtagtctt ccagacgctg ccgcatgcgc cagttgctgg cccgcttcgg
     2401 cgtctgccga atgaccatgc cgctggccca gatccgatcg gactccacgt cctccggatt
     2461 gatcccggtg ttaccgatct gcgggtaggt gaaggtgatg atctggcggg cgtacgacgg
     2521 atcggtgagg atttcctggt agcccgtcat ggcggtattg aaaacgactt ccccgaccga
     2581 tgtaccttct acaccaatcg acagaccttt aaacacactc ccgtcctcga gagcgagcag
     2641 tgcccgaacc aaatcgtttt ccaataacag gtcgcaaaaa ggcgagatcg cccagaaccg
     2701 atcccgcctt gaattcgggg cggtagtcta cccaagtgca cccttgaagc ccagcaca
//
LOCUS       DBNHQR010000371         5327 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_35559, whole genome shotgun sequence.
ACCESSION   DBNHQR010000371 DBNHQR010000000
VERSION     DBNHQR010000371.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5327)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_35559"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..169)
                     /locus_tag="AC16VR_08360"
     CDS             complement(<1..169)
                     /locus_tag="AC16VR_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility protein PilT"
                     /protein_id="HIR6011208.1"
                     /translation="MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLEH
                     QQVHSLIYEIMN"
     gene            335..1012
                     /locus_tag="AC16VR_08365"
     CDS             335..1012
                     /locus_tag="AC16VR_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011914971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HIR6011209.1"
                     /translation="MHSISHNVASVRSCVVSAARAAQRDPDEVCLIAVSKRQPARAIE
                     EAHGAGLKDFGENYLQEALPKIAALGHLPLTWHFIGALQSTKTRQVARHFDWVHTVDR
                     PKIARRLNEQCPDDKTLQVCLQVNIDADPGKAGVDPAAVADLLKELVDLERICVRGLM
                     TILDPNADAPESFARMAKLFAGLAPLAAGRWDTLSMGMSNDYEQAIMAGATHVRIGTA
                     IFGAREG"
     gene            1046..1855
                     /gene="proC"
                     /locus_tag="AC16VR_08370"
     CDS             1046..1855
                     /gene="proC"
                     /locus_tag="AC16VR_08370"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HIR6011210.1"
                     /translation="MSSHITFIGAGNMATSLIKGLIASGHDAATIRASDPAPEQLGKI
                     AELGVATSDDNNAQVRGADVVILAVKPQVMASVAGGLTALSTDQLLVSIAAGVPMSSL
                     AGWTSSEQPIVRCMPNTPALLGAGVTALCANERVSPAQRELAESVLATAGRTLWVSLE
                     DQLDAVTAVSGSGPAYFFYLMEAMIEAGQALGLSAETAVTLTLETARGAALMALEGPP
                     PAQLRANVTSPGGTTQAALEILDAADCRGIINAAVAAAAERSRELAIEFGR"
     gene            1886..2422
                     /locus_tag="AC16VR_08375"
     CDS             1886..2422
                     /locus_tag="AC16VR_08375"
                     /inference="COORDINATES: protein motif:HMM:NF014389.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HIR6011211.1"
                     /translation="MAEVLYYATSFILQVIAFLFVVRFLLQACRADFYNPISQGIVKA
                     TDVVLKPVRMVLPGYRNLDFAALLAALIVEVVMILALGAIADRPTGTAVQIATSGVLQ
                     MILLGLFIIKWSILIVIIASFLAPGNYHPALALLQQLTEPVLAPARKLLPPMGGLDFS
                     PILVFLILGVIERVLLQI"
     gene            2523..3677
                     /gene="metX"
                     /locus_tag="AC16VR_08380"
     CDS             2523..3677
                     /gene="metX"
                     /locus_tag="AC16VR_08380"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008174179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase MetX"
                     /protein_id="HIR6011212.1"
                     /translation="MSSSLPSDSVGLVSPQVFAHPSGFSLRCGETLEGFELVYETYGR
                     LNAARSNAILVCHALSGSHHAAGYHSEDDKRPGWWESCIGPGKPIDTDRFFVVCPNNL
                     GGCHGSTGPTTVDPRTGEPFGPNFPVVTVEDWVDTQALLADHLGIDRFAAVVGGSLGG
                     MQAMRWATDYPDRLHAAVLIACAARLSAQNIAFNEIARQAIISDPEFHGGNYRQLGVN
                     PDLGLMLARMVGHVTYLSDDGMRQRFGRQLKSGDIETAQDVEFQVESYLHHQGRSFSQ
                     RFDANSYVLMTRALDYFDPAREHDDDLVKALASATCRFCVISFTTDWRFSVARSKELV
                     DALIAAGKNVASAIIESEHGHDSFLLNVPRYFDVLRAYLGRIATEAVDAP"
     gene            3667..4275
                     /gene="metW"
                     /locus_tag="AC16VR_08385"
     CDS             3667..4275
                     /gene="metW"
                     /locus_tag="AC16VR_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008364885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HIR6011213.1"
                     /translation="MRPDLEIIAEVIKPGARVLDLGCGEGELLAYLQTSKQVNGYGLE
                     IDPDNITTCLHNGVNVIEQDLDEGLDNFPDDSFDLVVMTETLQAVQNPDRMLDDMLRI
                     GGECIVTFPNFGYWRCRAYLALRGRMPVSEHLPHAWYDTPNIHLCTFKDFERLCHSKG
                     LPVIDRFVVDERHLARRLMQRFPNFFGHIAFYRLGRPNSGEA"
     gene            4275..4712
                     /locus_tag="AC16VR_08390"
     CDS             4275..4712
                     /locus_tag="AC16VR_08390"
                     /inference="COORDINATES: protein motif:HMM:NF025822.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4426 domain-containing protein"
                     /protein_id="HIR6011214.1"
                     /translation="MVKTGAGWICALILSWAGTPGHAEQRQSFGGWDVHYVVLPTGFL
                     KPAIAAQYGITRGRDRALVNISVLDPTGAPQKVSIEGTVKNLLGQLQPLAFREVTEGQ
                     AVYYLAAVKHTHEEVLRFEISLSAPDAPPMQLKFQQKLYWEDT"
     gene            4720..5307
                     /gene="rdgB"
                     /locus_tag="AC16VR_08395"
     CDS             4720..5307
                     /gene="rdgB"
                     /locus_tag="AC16VR_08395"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018402585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HIR6011215.1"
                     /translation="MSIVLASNNPGKLAELRLLIAPLGLDAVSQRDLGIASAPETRAT
                     FIENALEKARHVSAAAGMPAIADDSGLAVPALGGAPGVRSARYAGEDATDAENNAKLV
                     AAMADMDDRRASFHCAVVFMRAPDDPVPVVATAAWSGRIVADPRGTGGFGYDPHFFID
                     ETDCTAAELDPSVKNEISHRGRAMRALCAALAGVV"
BASE COUNT          928 a         1800 c         1652 g          947 t
ORIGIN      
        1 cgttcatgat ctcgtagatc agcgaatgca cctgctggtg ctcgagcggc ggcaggttga
       61 tccggcgaac gtcaccgtca acacgaatca tcggcggaag ccccgcggac aggtggaggt
      121 ccgatgcgcc ctgcttggcg ctgaaagcga ggagttcggt aatgtccatc agcgatcctt
      181 gtctactcgc ttgggcttca aactcgccga aatggcaacg aaagccgctg cgaatccggg
      241 ctgtatagta ttatctgacc tgggctttaa ccatgaatcc ctcctcgctc tgccggagac
      301 atgcgaaatc tgcgacgcag ttggcaaaac gaaattgcac tcgatctctc acaacgtcgc
      361 cagcgtgcgt tcctgtgttg tctctgcggc gcgagccgca cagcgggacc ccgacgaggt
      421 ctgcctgatc gcagtctcca agcggcaacc ggcccgtgcc atcgaagagg cccacggcgc
      481 cggcttgaag gacttcggcg aaaactatct gcaggaagcg ctgccgaaga tcgccgcgct
      541 cgggcatctg cccctgacct ggcacttcat cggcgcgctg cagtcgacca agacccgcca
      601 ggtggcgcgg catttcgact gggtgcatac ggtggatcga cccaagatcg cccgccggct
      661 caacgagcag tgtcctgacg acaaaaccct gcaggtctgt ctgcaggtca acatagacgc
      721 cgatcccggc aaggccggcg tcgacccagc cgcggtcgcc gatctgctga aggagctcgt
      781 tgatctcgag cggatctgcg tgcgcggcct catgaccatc ctcgacccga acgcggatgc
      841 cccggagagc tttgcgcgca tggcgaagct gttcgccggg ctcgcgcccc tcgccgccgg
      901 tcgctgggat accctgtcca tggggatgag caacgactac gagcaggcca taatggccgg
      961 cgccacccac gtgcgcatcg gcaccgccat cttcggcgcg cgcgagggct aacccgaacg
     1021 agccactacc cggagttccg atccgttgag cagtcacatt actttcattg gcgccggcaa
     1081 catggcgacc agcctgatca aaggcctgat cgccagcggt cacgatgcgg cgaccattcg
     1141 cgccagcgac cccgcacccg agcagctggg caagatcgcc gagctcggcg tggcaacctc
     1201 cgacgacaac aatgcccagg tacgcggtgc ggacgtggtg atcctcgcgg tcaagccgca
     1261 ggtcatggcc tcggtagccg gggggctgac ggcgctgagc acggatcagc tcctggtctc
     1321 catcgccgcc ggcgttccca tgtcgagcct tgccggttgg accagcagcg agcagcccat
     1381 cgtccgctgc atgccgaaca cgccggccct tctcggcgcc ggagtcaccg ccctgtgcgc
     1441 caacgagcgc gtctcgccgg ctcaaaggga gctcgcagaa agcgtgctgg ccaccgcggg
     1501 caggaccctc tgggtatcct tggaagatca gctcgacgcg gtgaccgccg tctccggcag
     1561 cgggcccgcc tacttctttt acctgatgga agcgatgatc gaagccggcc aggccctggg
     1621 gctcagcgcc gagaccgccg tcaccctgac gctggaaacc gcccgcggcg ccgcgctgat
     1681 ggctctcgaa gggcccccac ccgcgcagct cagggcaaat gtgacctctc cgggcgggac
     1741 gacccaggcg gcgctggaaa tactggatgc cgccgactgc cggggtatca tcaacgccgc
     1801 tgtagcggcc gccgcggaac gatcccgcga gctcgccatc gagttcggtc gctaaacgtc
     1861 gtaatcaggg gaataggcaa cgagcatggc agaggtctta tattacgcaa ccagcttcat
     1921 tctgcaggtc attgccttcc tgttcgtggt tcgtttcctg ctgcaggcat gccgggccga
     1981 cttctacaac cccatttctc aagggatcgt caaagccacc gacgtggtgt tgaagccggt
     2041 ccggatggtg ctgcccggat accgaaatct ggatttcgcc gccctcctgg cagcgctcat
     2101 cgtggaggtc gtgatgatcc tcgccctggg ggccatcgcc gatcggccca cgggaaccgc
     2161 tgtgcagatc gcaacctcag gggtgctgca gatgattctg ctggggctgt tcatcatcaa
     2221 gtggtcgatt ctgatcgtga tcatcgcgag cttcctcgct ccgggtaact accacccggc
     2281 gctggccctg ctccagcagc tcacagagcc cgtcctggca ccggcccgca agctcctgcc
     2341 acccatgggc ggtctcgact tctcgccgat cctcgttttc ctgatcctcg gcgtcatcga
     2401 acgggtgctg ctgcagatct gagctaatcg gctgcgcccc ggccggatgc cccgaccgac
     2461 ccggttgatg gccttgaagc ccttgcttta gactcgccgt cccgttcttc gtggcctact
     2521 cagtgtcgag cagcttgcca tccgattccg tgggcctggt cagcccgcag gtcttcgcgc
     2581 atccctccgg cttttcgctt cgctgcggtg agacgctcga ggggttcgag ctcgtctacg
     2641 agacctacgg acggctcaac gctgcgcgga gcaacgcgat tctcgtctgc catgctctct
     2701 ccgggagcca tcacgctgcc ggctaccact ccgaggacga caaacgaccc gggtggtggg
     2761 aaagctgcat cgggcccggc aagccaatcg acacagaccg gttcttcgtc gtctgcccca
     2821 acaatctcgg cggctgccac ggcagcacgg ggccgaccac ggtcgacccc cgcacgggtg
     2881 aaccgttcgg gccaaacttt cccgtcgtca ccgttgaaga ctgggtagat actcaggccc
     2941 tgctcgccga ccacctgggc atcgatcgct tcgccgccgt cgtcggtggc agcctggggg
     3001 gcatgcaggc catgcgctgg gcaaccgact atcccgatcg ccttcacgcc gccgtgctga
     3061 tcgcctgcgc cgccaggctg tccgctcaga acatcgcttt caacgagatc gcacggcagg
     3121 ccatcatctc ggatccggag tttcacggcg gcaactaccg gcagctgggc gtcaacccgg
     3181 acctgggcct catgctcgcg cgcatggtag gtcacgtcac ctatctgtcc gacgatggca
     3241 tgcgccagcg cttcggccgg cagctgaagt caggggatat cgaaaccgcc caggacgtgg
     3301 agtttcaggt ggagagctat ctgcaccacc agggccgctc gttctctcag cgcttcgacg
     3361 ccaacagcta cgtgctgatg accagggcac tggactactt cgacccggcc cgggagcacg
     3421 acgacgacct ggtcaaagcg ctggcatccg ccacctgccg cttctgcgtc atctcgttca
     3481 ccacggactg gcgcttctcc gtggcgcgct cgaaggagct ggtggacgcg ctcatcgcgg
     3541 cgggcaagaa cgtggccagc gccatcatcg agtcggagca cggccacgac tcgtttctcc
     3601 tgaacgtgcc gcgatacttc gacgtgcttc gggcctatct gggccgcatc gccacggagg
     3661 ccgtcgatgc gccctgacct ggagatcatc gcggaggtga tcaaacctgg agcccgggtg
     3721 ctcgatctcg gttgcggcga gggcgagctg ctcgcttatc ttcagacttc aaagcaggtc
     3781 aacggctacg ggctggaaat cgatcccgac aacatcacca cctgcctgca caacggcgtc
     3841 aacgtcatcg agcaggatct cgatgagggg ctcgacaatt ttccggatga cagcttcgac
     3901 ctggtggtga tgaccgagac gctgcaggcg gtgcagaacc ccgaccgcat gctcgacgac
     3961 atgctccgga tcggcggcga gtgcatcgtc acgtttccga atttcggcta ctggcgttgc
     4021 cgcgcgtatc tggcgctgcg cggccgtatg cccgtctccg agcacctgcc tcacgcctgg
     4081 tacgacacgc ccaacatcca cctgtgcacc ttcaaggatt tcgaacggct gtgccacagc
     4141 aaaggcctac cggtcatcga tcgcttcgtc gtcgacgagc ggcatctcgc aagacggctg
     4201 atgcagcgat ttcccaactt cttcggtcac atcgccttct ataggctggg cagaccgaat
     4261 tcgggagagg cctgatggtg aagacgggcg ctggctggat ctgcgcgctc atcctgtcgt
     4321 gggcggggac gccgggacac gccgagcagc ggcagtcctt cggtggctgg gacgtgcact
     4381 acgtggtgct gccgaccggc ttcctgaaac cggccatcgc cgcgcaatac gggatcactc
     4441 ggggccgcga ccgcgccctg gtcaatattt ccgtgctcga cccgacgggc gctccccaga
     4501 aggtctccat cgaaggcacc gtgaagaatc tgttgggtca gctgcagccg ctggcgttcc
     4561 gggaggtcac cgagggccag gccgtctact atctggcggc cgtcaagcac acccacgagg
     4621 aggtgctccg gttcgagatt tcccttagcg ctcccgacgc gccgcccatg cagctcaagt
     4681 tccagcagaa gctgtactgg gaagacacgt gatcccgcgg tgagcatcgt tctcgccagc
     4741 aacaaccccg gcaagcttgc cgagttgcgg ctcctgatcg ccccgctggg gttggatgcg
     4801 gtcagccagc gcgacctcgg cattgcgagc gcaccggaaa cacgggccac gttcatcgag
     4861 aacgcgctgg agaaagcccg ccacgtcagc gccgcggcag gcatgccggc gatcgccgac
     4921 gactccggac tcgccgtgcc ggcgctgggc ggggcgcccg gcgtgcgctc cgcccgctat
     4981 gcgggcgaag atgccacaga tgcggagaac aacgccaagc tggtcgccgc catggccgac
     5041 atggacgacc gccgcgcaag ctttcactgc gccgtggtct tcatgcgcgc acccgacgac
     5101 cctgtacccg tcgtcgccac ggccgcctgg agcggtcgca tcgttgccga tcccaggggc
     5161 acgggcgggt tcggttacga tccgcatttc ttcatcgacg agacggattg cacggccgcc
     5221 gagctggacc cgtccgtgaa gaacgagatc agccaccgcg gtcgggccat gcgcgcgctg
     5281 tgcgccgcgc tggccggagt cgtttgatgg ggccggtcgg gttgtac
//
LOCUS       DBNHQR010000372         3877 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1111783, whole genome shotgun sequence.
ACCESSION   DBNHQR010000372 DBNHQR010000000
VERSION     DBNHQR010000372.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1111783"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="AC16VR_08400"
     CDS             complement(<1..537)
                     /locus_tag="AC16VR_08400"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR6011216.1"
                     /translation="MKYLARAILRLGGWRIEGAKPDLEKYVLVAAPHTSNWDFLWMIC
                     MAQALEIPVSWLGKDALFHPLIGWFFQLLGGIPVDRSAPSDLVSRLASRFASSSDLVL
                     LVPVEGTRGHGLHWKSGFYHIARAAQVPVVLGFLDYVKRTGGFGPMLVPTGEVASDMD
                     VPRGFYAGIAGKFPALASP"
     gene            complement(609..1490)
                     /locus_tag="AC16VR_08405"
     CDS             complement(609..1490)
                     /locus_tag="AC16VR_08405"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR6011217.1"
                     /translation="MCDEQTVRDNEAYLHTSAGMSRRQFAGAAAGAAIAMLLPRSGNA
                     LTVSGRDVRITTPDGEADAYFVHPAEGKHPGVIVWPDIRGLRPAFRQMADRLAGSGYA
                     VLVVNPFYRDARAPLVPEGASFQDEATRAVLMPLYNSITSTTTDVDAKALVDFLDAQT
                     EVDTTGGIGTMGYCMGGPMTLHSAAAVPDRVAAAASFHGSRLVTDAEDSPHLRVPGMS
                     ASFLIAIAENDDARDPQAKDVLELVFDDAGLAAEIEVYEGALHGWCPPDSSVYHEGQA
                     ERAWERLLVLFESALKS"
     gene            complement(1534..2568)
                     /locus_tag="AC16VR_08410"
     CDS             complement(1534..2568)
                     /locus_tag="AC16VR_08410"
                     /inference="COORDINATES: protein motif:HMM:NF013847.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen
                     decarboxylase/cobalamine-independent methonine synthase
                     family protein"
                     /protein_id="HIR6011218.1"
                     /translation="MAALESGITGSLPKPKWLSDPSAQLRADWMVADDALGEAHDDAV
                     RLAIADQVSAGLDIVTDGEQRRRHYIWGFLEALEGVDAHKLGKKQTRGARYTTELQNV
                     ARVVGDVRRSGPIFVDALKFAKAHSDRPVKVTLPGPMTIVDSVVDEHYGDALPDLAMR
                     FADILNAEARALADAGADIVQFDEPAFNVDTDAVAEWGIATLERAAEGVATQRAVHIC
                     YGYGVPQVIAWKNQNQDWGHYGHTLPLLNESSIDQVSVECAASGVDVSVIQHLSNKRV
                     MLGVISVSTDEVETPELVAGRIRAGLEFCDAERLMPCTDCGLVPRPRAVAQAKMAALA
                     AGTALVRDAL"
     gene            complement(2596..3138)
                     /locus_tag="AC16VR_08415"
     CDS             complement(2596..3138)
                     /locus_tag="AC16VR_08415"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6011219.1"
                     /translation="MSNAPPVNSRLHHNAYVTENMEAVRRFYEEVIGFPLIATWCEQA
                     ELFGRERTYMHCFFDVGGGESLAFFQFADPADAAEFGPELPSSPFRHIAMKVDQETQD
                     AIRDRLAAAGYGEPDTYVLNHGYCYSLYVVDPSKLIIEFTVDHEDVDQINADQRAKAH
                     AELARWLGGDHSPNNEAYHR"
     gene            complement(3161..>3877)
                     /locus_tag="AC16VR_08420"
     CDS             complement(3161..>3877)
                     /locus_tag="AC16VR_08420"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
                     /protein_id="HIR6011220.1"
                     /translation="SGGFNQSGVGEAVPEPGVEDLFDEQTADTYEIGLKATLMDGRVS
                     TSLAAYYTDFEGAYFFFYDPNTSTQNLGSIDEVTYAGVELEANAMLTDNFTAYLGFGY
                     VDSEIDEAANTDHEGNQAPLVSEYTLNVGLQYRQPLGAMNGRLELVGRVDYQRIGDTY
                     WEPANITKRSPVDLVDLRLGVEVPGDWALTVWAKNAFDEKYNAEFSPGPRPAPGVDPT
                     FNFLFKAPPARWGVDFTKRF"
BASE COUNT          680 a         1309 c         1219 g          669 t
ORIGIN      
        1 cggtgacgcc agggccggaa acttgcccgc gattccggca tagaagcctc tcggcacgtc
       61 catgtcggag gcgacctcac ccgtggggac caacatggga ccgaaaccac ccgtgcgctt
      121 gacgtagtcg agaaatccca gcaccacggg cacctgcgcg gctcgcgcaa tgtggtagaa
      181 gcccgatttc cagtggaggc cgtgtccgcg agtgccttcc accggaacca gcagcacgag
      241 atcgctcgaa ctcgcgaagc gggatgcgag ccggctcacc agatcagagg gagccgagcg
      301 gtccacggga atgccaccga gcagttgaaa aaaccagcca ataaggggat ggaagagcgc
      361 gtctttgcca agccaggaca caggaatctc cagcgcctga gccatgcaga tcatccacag
      421 aaaatcccag ttcgaggtgt gcggcgccgc caccagaacg tatttttcaa gatcgggctt
      481 ggcaccctcg atgcgccaac cgcccagccg cagaatggcg cgagccagat atttcactag
      541 agattccgag gggaggaccc ggcagtatag gacctgagtc ccttcactgc accaaatggc
      601 gcgacggatc agcttttcag cgctgactcg aacaagacca gcaaccgctc ccaggcccgc
      661 tcggcctgcc cttcatggta gacggacgag tcgggcgggc accagccgtg cagcgctcct
      721 tcgtagactt cgatctcggc ggccaggccc gcatcgtcga atacgagctc gaggacgtcc
      781 ttggcctgag ggtctcgggc atcgtcgttc tcggcaatgg cgatgagaaa gctcgccgac
      841 atgccgggca ctctcaggtg cgggctgtcc tcggcgtctg tgacgagcct gctgccatga
      901 aaggaagccg ccgccgcaac tcgatccggg accgcagctg ccgagtgcag ggtcatcggg
      961 ccgcccatgc agtaccccat ggtcccgatc cctcccgtcg tgtccacctc ggtttgggca
     1021 tcgagaaagt cgacgagcgc cttggcgtcc acgtcggtgg tggtcgacgt gatggagttg
     1081 tacagcggca tcagcacggc acgggtggcc tcgtcctgaa agctggcacc ttcgggaacc
     1141 aagggcgcgc gcgcatcccg atagaaggga ttgaccacca gcaccgcgta gcccgaacca
     1201 gccaggcggt cggccatctg ccggaacgcc ggccgcagtc cccggatgtc cggccacacg
     1261 attacccccg ggtgcttccc ttcggcagga tgcacgaagt acgcatcggc ctcgccgtcc
     1321 ggcgtggtga tccgaacgtc gcggcccgaa accgtcaggg cgttgccaga gcggggcagc
     1381 agcatcgcaa tcgccgcacc tgcagcggcc cccgcgaact ggcgccgtga catgccggca
     1441 gacgtatgca gatacgcttc gttgtcacga acggtttgct cgtcgcacat ggtcaagcct
     1501 ccctggccgg ccggcgacgg ccgagccaat cgatcagagg gcgtcccgca ccagcgcggt
     1561 gcctgcagcc agagccgcca tcttcgcctg ggccaccgcc cgggggcgcg gcaccagacc
     1621 gcagtccgtg caaggcatga ggcgctcggc gtcgcagaac tcgaggccgg cccggatgcg
     1681 cccggccacc agctccggcg tttccacctc gtcggtgctc acgctgatca cgcccagcat
     1741 cacgcgcttg ttggacagat gctggatcac cgagacgtcg accccggacg ccgcgcactc
     1801 cacggacacc tgatcgatgc tggactcgtt cagcagcggc agcgtgtgcc cgtagtgccc
     1861 ccaatcctga ttctgatttt tccaggcgat cacctggggc acgccgtagc cgtaacagat
     1921 gtgcacggcg cgctgcgtcg ccacgccctc cgccgcccgc tccagcgtcg cgatgcccca
     1981 ctcggccacc gcatcggtgt ccacgttgaa ggcgggctcg tcgaactgga cgatgtctgc
     2041 tccggcgtcg gcgagcgctc tggcctccgc gttgaggatg tcagcaaacc gcatggcgag
     2101 atcgggcagc gcatcgccat agtgctcgtc caccaccgaa tccacgatgg tcatgggtcc
     2161 gggcagggtg acctttaccg gccggtcgga gtgggccttg gcgaacttca gagcgtccac
     2221 gaaaatgggg ccggaccgcc gcacgtcccc aacgacgcgc gcgacgttct gcagctcggt
     2281 ggtgtaacgc gccccgcgcg tctgcttctt gcccagctta tgcgcgtcca cgccctccag
     2341 cgcctccagg aacccccaga tgtagtggcg ccggcgctgc tcgccgtccg tgacgatatc
     2401 caggcccgcg cttacctgat cggcgatggc gagccggact gcatcgtcgt gtgcctcgcc
     2461 gagagcgtcg tccgcgacca tccagtccgc gcgcagctgc gccgaagggt cgctcagcca
     2521 cttcggtttc ggcaggctgc cggtaatgcc actttctaga gccgccatgg gcctttctcc
     2581 gggaacttcg tgatcctagc ggtggtaggc ctcgttgttc ggcgagtgat cgccgccgag
     2641 ccaacgggcg agctccgcat gagccttggc gcgctggtcc gcgttgatct gatccacgtc
     2701 ctcgtgatcc accgtgaact cgatgatgag cttgctgggg tcgacgacgt agagcgagta
     2761 gcagtagccg tgattgagca cgtaagtgtc cggctcccca tagcccgccg ccgcaagccg
     2821 gtcgcgaatg gcgtcctgag tttcctgatc caccttcatg gcgatgtgcc ggaacggcga
     2881 ggaaggcagc tcaggcccga actcggcggc atccgccgga tccgcgaact ggaagaacgc
     2941 gagactctca ccgccgccca cgtcgaagaa gcagtgcatg taggtgcgct ccctgccgaa
     3001 gagctcggcc tgctcacacc aggtggcgat cagcggaaag ccgatcacct cctcgtagaa
     3061 cctgcgcacc gcttccatgt tctcggtgac gtaggcgttg tggtgcagac gtgaattgac
     3121 cggtggtgcg ttgctcatga tgtcctcgct attgcccagc tcagaaccgc ttggtgaaat
     3181 ccacgcccca gcgcgccggc ggggctttga acaggaaatt gaatgtcggg tctacgcccg
     3241 gtgccggcct cggccccggg gagaactccg cgttgtactt ctcgtcgaac gcgttcttgg
     3301 cccagaccgt gagcgcccag tctcccggga cctcgacgcc cagccgcagg tccaccagat
     3361 ccacggggct gcgcttggtg atgttggccg gctcccagta agtatcgcca atgcgctggt
     3421 agtccacccg gcccaccagc tccagccgac cgttcatggc gcccaggggc tggcggtact
     3481 gcaggccgac gttgagcgtg tactcggata ccagcggtgc ctggttgccc tcgtggtcgg
     3541 tatttgccgc ctcgtcgatc tcgctgtcca cgtacccgaa gcccaggtag gcggtgaagt
     3601 tgtcggtgag catggcgttc gcttcgagtt cgacaccggc gtaggtcacc tcatcgatgc
     3661 tgcccaggtt ctgcgtggac gtgttcggat cgtagaagaa gaagtaggca ccctcgaagt
     3721 ccgtgtagta cgcggccagc gacgtgctga cccgcccgtc catcagcgtt gccttcaggc
     3781 cgatctcgta ggtgtccgcc gtctgctcgt cgaagagatc ctcgactccg ggctcgggaa
     3841 ccgcctcgcc tacgcccgac tggttgaacc cgccgct
//
LOCUS       DBNHQR010000373         3393 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1363490, whole genome shotgun sequence.
ACCESSION   DBNHQR010000373 DBNHQR010000000
VERSION     DBNHQR010000373.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1363490"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="AC16VR_08425"
     CDS             <1..286
                     /locus_tag="AC16VR_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011221.1"
                     /translation="ELMPAARALACEIADNSSAISVTLVRQMMWRMLGADHPMEAHKI
                     DSRGVYFTGKSPDAREGVEAFLEKRPARFPGKVSSDLPEFFPWWEERTFS"
     gene            297..536
                     /locus_tag="AC16VR_08430"
     CDS             297..536
                     /locus_tag="AC16VR_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HIR6011222.1"
                     /translation="MSSPTIAQKAPYAVDVQAGRKYFWCACGRSAKQPFCDGSHEGTA
                     LTPLPYTAEKDATLYFCGCKHTKNVPLCDGSHNDL"
     gene            552..3341
                     /locus_tag="AC16VR_08435"
     CDS             552..3341
                     /locus_tag="AC16VR_08435"
                     /inference="COORDINATES: protein motif:HMM:NF015926.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HIR6011223.1"
                     /translation="MLSRIFGNKDPLSQPDPAARRRAVEGLSEGKAGELQERLLELAR
                     SDADPGVRQACVSRLQGTSDLEQLLDDGLMANRAAERIAELLGPDASSELTRHPLVLR
                     ARLFRLAPEDAARLIDSIEDEDTLVDLTAGSREPLRGRILAGIRSARALAALEQQSRG
                     RDKGLNRFAREGMERIKQLRREAQATLDRLNELADALERASRQAPGIAARQRYESLLR
                     EFDQAYERFAGHARALEGCNEPPRDASPIRARVAALVAPAEPVAISRQEETPASGGEA
                     FADLVPQLEALEGLMEAGHDFQTVSETRSRLTEAWLRAADHALPGDAEHRVFERVSHA
                     YQNLADAAGRLTAAGWQTHRDPLPSDLPDDPGEAAGFWQAAEERRRLLKRAEGLVRAV
                     AWPDWASPTAELRDLLDDVERLREEVARSDALGSSRHEDLKMLISTAASAIDEGAVRA
                     ATEALGRARAILQALPARGREAQARALQQESARLSELRDWQTFATTPKREALCEAVQN
                     LADQPLDPPDQAERIKALRGEWNGLGPVSGREDRALADRFNRLAEQAFEPCRAYFAEQ
                     ASVRASNLVERGKICDQLENYLESTDWKAADIKAAERILRAARDEWRRFQPVDRNPGK
                     PVEERFEVLQERLHKLIKAEWDANLQLKRQIVEEAEALAGSEADPHDRAKEAKALQRR
                     WREIGITPRGPDRKLWQQFRGACDAIFDALGAAREASHQAVSEALDAADNLLTSFESD
                     LGNTSATEAEAEILTGFRRRFSELPPLPEREGRQISRRFEELTRSYRSLLHQKSRYQE
                     RAELDRLRALDEAVTHTEIAPGLTAEPTQDAIADPVVSERLQKSGEPIPMDALRALTV
                     RAEILAGAESPAQDREIRLKLQVEEMNVGMGSQRRAQDPLEMAREWCARGPKDEACSA
                     LRERFFDALARALED"
BASE COUNT          617 a         1116 c         1187 g          473 t
ORIGIN      
        1 ggagctgatg ccggcagcaa gagccctcgc ctgcgagatc gccgacaatt cttccgcgat
       61 ctcggtgacg ctggtgcgcc aaatgatgtg gcgaatgctg ggcgcggatc atcccatgga
      121 agcccacaag atcgactccc gtggggtgta cttcaccggc aagtctccgg acgctcggga
      181 gggcgtcgaa gcgttcctgg aaaaacggcc cgcccggttc ccgggcaagg tcagcagcga
      241 tctgccggaa ttcttcccct ggtgggagga gagaacgttt tcatgaggac aacgacatga
      301 gctcaccgac cattgcccag aaagcaccct acgcggtaga cgtgcaggcc ggccgcaagt
      361 atttctggtg cgcctgcgga cgcagcgcca agcaaccctt ctgtgacggt tcccacgagg
      421 gcacggcgct gaccccgctg ccgtacacgg cggaaaagga cgctacgctc tacttctgcg
      481 gttgcaagca caccaagaac gttcccctct gcgacggctc ccacaacgac ttgtgatcgt
      541 gaaggatcgc catgctgtcg cgaatcttcg gaaacaagga ccctctgagc cagccggatc
      601 cggcggcccg gcggcgcgcg gtcgaggggc tgtcggaagg taaagcgggc gagctgcagg
      661 agcggttgct cgagcttgcg cgcagcgacg cagatcctgg cgttcgccag gcctgcgtga
      721 gccggctgca gggaacatcg gacctcgagc agctgctcga cgacgggctg atggcgaacc
      781 gtgcagctga gcggatcgcc gagctgctcg gccccgacgc ctccagcgag ctgaccagac
      841 acccgctcgt ccttcgcgcc cgcctgttcc gcctggcgcc cgaggacgca gcccgcctga
      901 tcgattccat agaggacgag gacacgctgg tggatctgac ggccggctcg cgggagccgc
      961 tgcgcggaag gattctggcg ggaatccgat cggccagggc gctggcggct ctggagcagc
     1021 agagccgcgg tcgggacaag gggctcaatc gcttcgcccg ggaaggcatg gagcgcatca
     1081 agcagctcag gcgcgaggcg caggccacgc tggaccgcct gaacgagctt gccgacgccc
     1141 tcgagcgcgc atcgcggcag gcgcccggca tcgccgcacg acaacgctac gagtcactgc
     1201 ttcgggagtt cgatcaggct tacgagcgtt ttgccgggca cgcccgggcg ctggagggtt
     1261 gcaacgaacc gcctcgggac gcgtcaccca tccgagcccg cgtggccgct ctcgtcgccc
     1321 ccgcggaacc ggtggcgatc agccggcagg aggaaacacc ggcgagcggt ggggaggcgt
     1381 ttgccgacct cgtgccgcag ctggaagcgc tggaggggct gatggaagcg gggcacgact
     1441 tccagacggt atccgagacc cggtcccgtc tgaccgaagc ctggctgcgc gccgccgacc
     1501 acgcgctgcc gggcgatgca gagcatcggg tgttcgagcg cgtctcccac gcctatcaga
     1561 acctggccga tgccgccggg cggctgacgg cggccggctg gcagacgcat cgtgatcccc
     1621 tgcccagtga cctgcccgat gatccggggg aagcagccgg cttctggcag gcggcggagg
     1681 agcggcgcag gttgctcaag cgcgcggagg ggctcgttcg agccgttgcc tggccggatt
     1741 gggcgagccc cacggctgag cttcgagacc tgctcgacga cgtggaacga ctgcgggagg
     1801 aggtggcgcg gtcagacgcc ctcggcagca gtcggcacga ggacctcaag atgctgatca
     1861 gcaccgcggc gagcgccatc gatgagggcg ccgttcgagc ggccacggag gccctcggcc
     1921 gcgcccgggc aattctgcag gcccttcccg cgcgtgggag ggaagcccag gccagagccc
     1981 ttcagcagga gtcggccagg ctgtccgagc tgcgtgactg gcagactttc gcgaccacgc
     2041 cgaagcggga ggccctttgc gaagccgttc agaacctcgc cgatcagccc ctggatccgc
     2101 cggatcaggc cgagcgaatc aaagcgctgc gcggcgagtg gaacggtctc gggcccgtgt
     2161 ccggacgcga agatcgcgcc ctcgccgatc gtttcaaccg cctcgccgag caggcattcg
     2221 agccgtgccg agcctacttc gccgagcagg ccagcgtacg cgcaagcaac ctggtcgagc
     2281 gcgggaagat ctgcgatcag ctggaaaact acctggaaag caccgactgg aaagcggcgg
     2341 acatcaaggc ggccgagaga attctgcgcg ccgcccggga tgagtggcgc agattccaac
     2401 ccgtcgaccg gaacccgggc aaacccgtcg aggagcggtt cgaggtgctc caggaacggc
     2461 tccacaagct catcaaagcc gagtgggatg ccaacctgca gctgaaacgg cagatcgtcg
     2521 aggaggcgga agcacttgcc ggcagcgaag ccgaccccca cgaccgggcc aaggaggcaa
     2581 aagcgctgca acggcgctgg cgggagatag gcatcacgcc cagaggcccc gaccgaaagc
     2641 tgtggcagca gtttcgcggc gcgtgtgatg cgatcttcga tgcgctggga gccgcccggg
     2701 aggcatccca ccaggcggtc agcgaggccc tcgacgctgc tgataacctg ctgacctcct
     2761 ttgagagcga ccttgggaat acgtcagcaa ccgaggccga ggcagagatc ctcaccggct
     2821 tccgacgccg cttcagcgag ctgccgccac tgccggagcg ggaggggcgg cagatttccc
     2881 ggcgcttcga ggagttgacc cgcagctacc gatcccttct gcatcagaag tcccgctacc
     2941 aggagcgggc cgagctcgat cgcctcagag ccctggacga ggcggtgacc cacacggaga
     3001 tcgcgccggg cctgacggcg gagccgacgc aagatgctat cgcggatccc gtcgtctccg
     3061 agcgtctgca gaaaagcggc gaaccgatcc ccatggacgc ccttcgcgcg ctcaccgtgc
     3121 gggcggagat cctcgccggc gcggaatccc cggcccagga ccgggagatt cgcttgaagc
     3181 ttcaggtcga ggagatgaac gtcgggatgg gcagccagcg gcgtgctcaa gaccctctgg
     3241 agatggcccg cgagtggtgc gcccgcggtc ccaaagacga ggcgtgctcg gcgctgcgcg
     3301 agcggttctt cgacgcgctc gcacgggcgc tggaggacta ggcgggcagg tccaggcaga
     3361 tgcgggcgcc ccccagggcg ctggtctcga tac
//
LOCUS       DBNHQR010000374         2700 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_541881, whole genome shotgun sequence.
ACCESSION   DBNHQR010000374 DBNHQR010000000
VERSION     DBNHQR010000374.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_541881"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="AC16VR_08440"
     CDS             complement(<1..228)
                     /locus_tag="AC16VR_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011224.1"
                     /translation="MSDARSVADYHFLKLNDEVLQNPYPFFRRLREEGGVVREADYDT
                     FLVGSLDAIREVNRQPEVFSSVLVANAPYVPL"
     gene            complement(225..1043)
                     /locus_tag="AC16VR_08445"
     CDS             complement(225..1043)
                     /locus_tag="AC16VR_08445"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6011225.1"
                     /translation="MPITIANGIDLYFERRGDGAPLLFISGSHGDLRARPNQFDTPLA
                     RAFDMIAYDQRGLGQTGKPPGEYTMAQYADDAAALLDALRLDAVPVVGVSFGGMVAQE
                     FALRHPDRVDRLILACTSSGGAGGSSYPLHELQALPPRERAEAHLRISDLRHDDEWVA
                     ANPERWERLVELTMAARRPDRDEQGSAKQLRARKGHDTYDRLPRLDVPVLLAGGRRDG
                     IAPVANMEALHRQIAGSRFELFDGGHMFLIQDKTAYPFIVGWLQNDWSELRDEP"
     gene            complement(1145..1220)
                     /locus_tag="AC16VR_08450"
     tRNA            complement(1145..1220)
                     /locus_tag="AC16VR_08450"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1185..1187),aa:Ala,seq:ggc)
     gene            1380..2615
                     /locus_tag="AC16VR_08455"
     CDS             1380..2615
                     /locus_tag="AC16VR_08455"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR6011226.1"
                     /translation="MTKNEAGRPELKRALNLPMLTLYGLGTTIGAGIYALLGEIAGTA
                     GYGAPFSFLIASLLAAFTACSFAELAARFPRAAGAALYVQKGLGTERLSTVVGLLVVA
                     SGLVSSAALVNGFQGYLQEFVELDRTIAIPAICAALAAIAAYGIVQSVTVAAAITVVE
                     IGGLILVIAVGADNFAALPERWPLMLPGTATTSWVGVLMGVSLAFYAFIGFEDMVDLA
                     EEVKNVRLNMPRGILLTLGITTVLYFVLMISALLALTPEELAASSAPMARLFEAGTGN
                     TSMLISVIALFAIINGALIQLVMVSRVLYGLGSRRKLPQWFARVNPKTRTPLVATLTA
                     AGVLFVLALSGHLAALAKTTSLLMLTIFMLVNLALWRIKSTEAQAPDDLPTFPRIVPA
                     MGALLSGLFVLRELSTRLF"
BASE COUNT          466 a          890 c          825 g          519 t
ORIGIN      
        1 cagcggcacg tagggcgcgt tcgctaccag gacgctcgag aacacttccg gctggcggtt
       61 gacttcacgg atggcgtcaa gagaaccaac gagaaacgtg tcgtaatccg cctcgcggac
      121 tacacccccc tcctcgcgca gccggcgaaa gaaaggatag ggattctgca gaacctcatc
      181 attgagcttg agaaagtggt agtcggctac agatcttgca tcgctcacgg ctcgtccctt
      241 aactcactcc agtcgttttg gagccatccc acaataaacg gataggcggt cttgtcctga
      301 atcaggaaca tgtggccgcc atcgaaaagc tcgaaccggc ttccggcaat ctgccggtgc
      361 agggcttcca tgttggcaac gggcgcgatg ccgtccctcc tgccgccggc cagaagaacg
      421 ggaacgtcga gtcggggcag acgatcgtac gtgtcatgcc ccttgcgggc ccggagctgc
      481 ttggccgagc cctgctcgtc tctgtccggg cgtcgcgcag ccatcgtcag ctccaccagc
      541 ctctcccagc gttccgggtt cgctgcaacc cactcgtcgt cgtgacgcag atcggagatc
      601 cggagatggg cttcggctct ctcccgggga ggcagcgcct gaagctcatg gagcgggtaa
      661 gaggatcccc cagcgcctcc cgacgacgtg cacgcgagaa tcaggcggtc gacacggtcc
      721 ggatgacgaa gcgcgaactc ctgcgcgacc atgccgccga acgaaacgcc gaccacggga
      781 accgcatcga gccgcagcgc gtccagcaat gcggccgcgt cgtcggcata ttgcgccatg
      841 gtgtactctc ccggcggctt gccggtctgg ccgagccctc tctggtcgta ggcgatcatg
      901 tcgaaggccc gggccaacgg ggtatcgaac tgattcggcc gggcgcgcag gtcgccgtgg
      961 ctcccgctga tgaacagcag cggcgcgcca tcgccccgac gctcgaagta gaggtcgatc
     1021 ccgttggcta tggtgattgg catgctggcc ctcggcgctg acacgttcgt ttatatgcca
     1081 agcgccgcat caaggccacc ggtcaggaaa ggcggggtct ccaacctcaa gtcattgatt
     1141 taattggtgg agccagacgg gatcgaaccg tcgacctcta caatgccatt gtagcgctct
     1201 cccagctgag ctatggcccc acgatgccgt ctcggccgag acgggaggag ggagcggatg
     1261 gtaagaaccc ggcgatccgg agtcaacatg tcaccctggc cgagccggtc gcagaatccc
     1321 tggatgcatc ccatcgattc cccgcctatg gttgggtcct gctccaggaa gcagggaaca
     1381 tgaccaagaa cgaggcaggc cgaccagaac tcaaacgtgc gttgaacctg ccaatgctca
     1441 cgctctacgg gttgggcacg accatcggcg caggaatcta cgctctgctt ggcgagattg
     1501 ctggcaccgc cggttacggg gcgcccttct ccttcctgat cgcatcgctt ttggcggcct
     1561 tcaccgcgtg ttcgtttgcg gagctggccg cgcgattccc gcgtgccgcc ggcgcggcgc
     1621 tttacgtgca gaagggcctg ggaaccgaac gcctttccac cgtggttggg ctgctcgtcg
     1681 tcgcctcggg actggtttcc tctgccgcgc tggtgaacgg ctttcaaggc tatctgcagg
     1741 aattcgtaga gctcgaccgg accatcgcca tcccggcaat ctgcgccgcg cttgccgcca
     1801 ttgcggccta cggaatcgtg cagtcggtga ccgtcgcagc ggcgataacc gtcgtggaga
     1861 tcggcggcct gattctggtc atcgccgtgg gcgccgacaa cttcgctgcg ctgcccgagc
     1921 gctggccact gatgttgccg ggcacggcca ccacttcgtg ggtaggcgtc ctcatgggcg
     1981 tcagcctcgc gttctacgcg ttcatcggct tcgaggacat ggtcgatctc gccgaggagg
     2041 tcaagaacgt caggctcaac atgccgcgcg gcatattatt gaccctgggc atcaccaccg
     2101 tgctctactt cgtgctcatg atctcggccc tcctcgccct gacgcccgag gagctggccg
     2161 ccagcagcgc ccccatggcc aggctcttcg aggccggcac cggaaacacc tccatgctca
     2221 tcagcgtcat cgcgctgttc gccatcatca acggcgccct gatccagctc gtgatggtct
     2281 cccgcgttct gtacgggctg ggttctcgcc gcaagctgcc tcagtggttc gcacgcgtga
     2341 acccgaagac gcgcacaccc ctggtggcaa ccctcaccgc tgccggcgtg cttttcgtgc
     2401 tcgctctgtc cggccatctg gcagccctcg ccaagacaac gtcgctgctc atgctgacga
     2461 tcttcatgct ggtgaatctg gcgttgtggc gtatcaaaag cacagaggcg caggcgcccg
     2521 acgacctgcc cacgtttccg cgcatcgtcc cggcaatggg tgcgctgctc agcggcttgt
     2581 tcgtgttgcg ggagctgtct acccggctct tctgactgcc ggtgagatca tcacttgcag
     2641 atcacgacgg ccggtcatgg gcgcgtcgag agatactcct ggtagcgctt ctccagccgt
//
LOCUS       DBNHQR010000375         9559 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_414806, whole genome shotgun sequence.
ACCESSION   DBNHQR010000375 DBNHQR010000000
VERSION     DBNHQR010000375.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9559)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9559)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9559
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_414806"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1296)
                     /gene="cgtA"
                     /locus_tag="AC16VR_08460"
     CDS             complement(106..1296)
                     /gene="cgtA"
                     /locus_tag="AC16VR_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016713347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HIR6011227.1"
                     /translation="MQFIDEATIRVEAGKGGGGCLSFRREKYVARGGPDGGDGGDGGD
                     VILVADDSLNTLVDFRYQPLYRAGNGQPGSGRNKTGAHGDARLVRIPVGTSVIDDETL
                     ETLGDLTEAGQELLVARGGRRGLGNAHFKSSVNRAPRRTTTGEPGEVRRLRLQLKLLA
                     DVGLLGLPNAGKSTLIAQVSAARPKVADYPFTTLVPSLGVVGAGTDNSFVMADIPGLI
                     QGAAEGSGLGVQFLRHLARTRILLHLVEVAPLDGSDPIDNARLIEAELADYSQALRER
                     PVWLVLSKTDLVTDADLEGLERRFADAFPGRPLFAVSAVTTAGIGRLTDALLAAVAGI
                     RERLAGDEEYAEREAELQARIGEDVLRGAVLAKPGALRGNSEERDEADDDGEDQVQVT
                     YVPE"
     gene            complement(1345..1614)
                     /gene="rpmA"
                     /locus_tag="AC16VR_08465"
     CDS             complement(1345..1614)
                     /gene="rpmA"
                     /locus_tag="AC16VR_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020291029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HIR6011228.1"
                     /translation="MAHKKAGGSTRNGRDSESKRLGVKKYGGQFVAAGNIIVRQRGTK
                     FHPGDNVGCGRDHTLFATADGYVQFLTRGPHKRKFVCVEPATAEA"
     gene            complement(1632..1943)
                     /gene="rplU"
                     /locus_tag="AC16VR_08470"
     CDS             complement(1632..1943)
                     /gene="rplU"
                     /locus_tag="AC16VR_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HIR6011229.1"
                     /translation="MFAVFSSGGKQHRVTEGEVIRVERLAGEPGDEVVFDKVLMVTDG
                     DDVSVGQPFVDGGKVTAEVVSSGRGKKIRVIKFKRRKDYMRRQGHRQWYTELKITGIN
                     G"
     gene            2134..3159
                     /locus_tag="AC16VR_08475"
     CDS             2134..3159
                     /locus_tag="AC16VR_08475"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014821448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR6011230.1"
                     /translation="MPGDPAIATTTTRISADPNDALTPVYDLARDDFAEVNRLIPHQL
                     TSDVELVEEIGRYIVDSGGKRLRPLMVLLGARCCGYEGDDHVRLAAIIEFLHTATLLH
                     DDVVDRSELRRGRATANALWGNAPSVLVGDFLYSRAFQLMVELEDMDIMAILADATNT
                     IAAGEVQQFASIGNFAIDEAQYMEIIRCKTALLFQAATHTAGVLSSEDPDEVAALKNF
                     GLHFGLAYQLVDDWLDYEGDSSTLGKNVGDDLAEGKLTLPLIHALANAPDADANVVRD
                     AVSARSAERLAEVLQVVRSSGALSYTRSAATRQCARAVECLTGLPDNEYRDALETLAN
                     FCLDRLR"
     gene            3204..4619
                     /locus_tag="AC16VR_08480"
     CDS             3204..4619
                     /locus_tag="AC16VR_08480"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6011231.1"
                     /translation="MPFASALAAAPALEQCELSGSHGVGRVEAVCGRLPRPENPEQPD
                     GARLDLFIAKIPSLSPEPAPDAFTIINGGPGASSVSLFVDLAQAFSGVLRERDIVLVD
                     QRGTGRSNALECPELEKAAEDYSIEAVQEATRACLESLAGDPRFYTTSQAVQDLEAVR
                     AALGYERLNVYGVSYGTRVSMHYLRRYPDRVRTLILDGVVPPGLALGVNVAENAQRRL
                     DELFAQCDRAPECSEAFPDLQASFTQLQSNLNAEPVSLRFPNPITGLEESLAFGYDHL
                     ALVVRLLSYAPETASLLPLIIDQAAGGNYLPAASQALRITDALSGTMSFGMHNSVTCT
                     EDLPFIDFSQVNWDALEATYIGADQVRALRAICEIWPRGAMDPDLKDPVQADTPTLIL
                     SGELDPITPPAYGDQIKPGFGNSMHIIGAGQGHGVVSRGCLPGLVTQMVEAGHLGDLD
                     AGCVERLSAAPFFIDLMGPAP"
     gene            4616..5341
                     /locus_tag="AC16VR_08485"
     CDS             4616..5341
                     /locus_tag="AC16VR_08485"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6011232.1"
                     /translation="MIRAEGLDKSFGEVTAIRHVSFDAADGKVTALLGPNGAGKSTSL
                     RILATVLQPDGGKAEVDGVDVVADPMAVRRQIGVLPHAAGLYARLTARENVAYFGSLH
                     GIAKSRLSERIDELIELLEMGEFAERRVAGFSQGQRIKVALARALVHDPRNVLLDEPT
                     NGLDVMATRALRDIIIRLRDEGRCVLFSSHIMQEVANVCDDVIVIGHGRVLFQGTLDG
                     LRDHAGEADLEEAFIRVIDEEDV"
     gene            5338..6510
                     /locus_tag="AC16VR_08490"
     CDS             5338..6510
                     /locus_tag="AC16VR_08490"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6011233.1"
                     /translation="MTGFAIVLAKECRDNIRDRRTIISSLSLAVLGPALFVGLMAFVL
                     DSAVGESSDPVEITVVGADHAPGLMAFLETQNTRIERVDLEDPRGAVAGGSRKLVLSI
                     PDDYSERFAAGQVTTVSLIYDSSDMGMSRRNFRMAQQFILAHARMIGLLRLQLRGVDP
                     AIASPVQVQAVDTASPAARALAILATWPYLLVLVIFMGGFYLAIDATAGEREHGSLEP
                     LLTQPISRGALVLGKVAATAVFSAASLALFLASLAISLPYAPLHKVGMSLHIDVSTAL
                     TVFVMALPLILFAAALLNVVASFAKSYKEAQTYLTFVILIPTLPLIITRMMNVEPSAA
                     LMMIPSLSQGTLINDAVAGEPVQWVFLLISVVSTTLTAGALTMLAVYLYKRERILI"
     gene            6565..6641
                     /locus_tag="AC16VR_08495"
     tRNA            6565..6641
                     /locus_tag="AC16VR_08495"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6599..6601,aa:Pro,seq:cgg)
     gene            6763..7023
                     /locus_tag="AC16VR_08500"
     CDS             6763..7023
                     /locus_tag="AC16VR_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011234.1"
                     /translation="MPDDELKRARDDARCEAAIQTLTDVLWRFEETCEGSYTLYDLVG
                     YLVEDLVREGCCAACIAESVTAAFKDIGADTTEHRRDEGTIH"
     gene            7096..7866
                     /locus_tag="AC16VR_08505"
     CDS             7096..7866
                     /locus_tag="AC16VR_08505"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6011235.1"
                     /translation="MSLFRLDNKNIAITGASSGFGHHFAGVLSEAGASVVLGARREDK
                     IRDRVDEINAAGGKATAVKLDVRDADSVRGFLEAAEAAYGPLDVVINNAGVEAGAKTY
                     LMIDEDDWDFVMDTNLKGAWLVSKTYTELVLAAGRKQGNIINISSITDTRTIKGQFPY
                     AVSKGALTRMTEVMALEAAKYGIRVNALAPGYILTDVSRLLLESDQAPEFVKGIPMRR
                     FGEFSDLDGPLLLLASDASTYMTGSVVVVDGGHICASL"
     gene            7893..9032
                     /locus_tag="AC16VR_08510"
     CDS             7893..9032
                     /locus_tag="AC16VR_08510"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017255227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase family protein"
                     /protein_id="HIR6011236.1"
                     /translation="MTDVRPFRIEIPEKDLDDLGQRLALTRLPEKETPDDWSQGVPLA
                     YMREIVDYWQSDYDWRRAEEEINRFPQFTTELDGLNVHFIHVKSPEPNALPLVMTHGW
                     PGSIVEFLKVIGPLADPASHGGDPADAFHVVCPALPGYGFSGKPITPGWGVEKIGSEW
                     ANLMARLGYESYVAQGGDWGSAVTTAIGMQDPDHCRAIHLNMVVAQPDPATMDDLTDL
                     EKRGIAGMQHYQQWDSGYSKEQSTRPQTIGYSLVDSPVGQAAWIMEKFWAWTDCDGHP
                     ENALSRDEMLDNIMLYWLTASGASSARLYWESFGSFSAGDVSVPTGCSIFPKEIFTCS
                     RRWAEQRFKNIVYWNELDRGGHFAAFEQPELFVGELRNCFRQVRG"
     gene            9029..>9559
                     /locus_tag="AC16VR_08515"
     CDS             9029..>9559
                     /locus_tag="AC16VR_08515"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6011237.1"
                     /translation="MTEGPIWRPDPVRALESNMARFTEEAARVSGRSMTGYADLHAWS
                     IEEPAAFWDLVWHFTDVRASQAPTAILTNADQLPGARWFEGARLNFAENLLRFDDDRP
                     ALIALLETGERREISYAELRRETAQVAAWLKNHIDPGDRVAGWLPNVPEAVTAMLATT
                     SLGGVWSSCSPDFGAEG"
BASE COUNT         1650 a         3261 c         2984 g         1664 t
ORIGIN      
        1 ccagcccagc gccgtcgtcg gtcagcagcg cgctgccgat cttgaccacg acccgtcggg
       61 cggcggccag ccgcgcacgc acctcgtcac tcggaatatc cctgctcatt cgggaacgta
      121 cgttacctga acctgatcct cgccgtcatc gtccgcttcg tccctctcct cggagttccc
      181 ccgcagcgca ccgggctttg cgagcacggc gccgcgcagc acgtcctccc cgatccgggc
      241 ctgcagctcc gcctcccgtt cggcgtactc ttcgtcaccg gcaagacgct cgcgaattcc
      301 ggccacggca gccagcagcg cgtccgtcag cctgccgatt ccagcggtgg tgacggccga
      361 cacggcaaag aggggacgcc cgggaaaagc gtcggcgaaa cgccgctcaa ggccttcgag
      421 atcggcatcc gtaaccaggt ccgtcttgct gagcacgagc cagacgggcc gttcgcgcag
      481 tgcctgactg tagtcggcca gctccgcttc gatgagcctc gcgttgtcga tggggtcgga
      541 gccgtccagc ggcgcgacct ccaccaggtg cagcagaatc ctggtacgcg caagatggcg
      601 cagaaactgg accccgaggc cggatccttc cgcagcgccc tgaatcaatc cgggaatgtc
      661 cgccatgacg aagctgttgt cggtaccggc gcccacgacc cccaggctgg gaacgagcgt
      721 ggtgaaggga taatccgcca ccttgggccg cgccgcggag acctgcgcga tcagcgtgga
      781 cttgcccgcg ttgggcagcc ccagcagccc gacgtcggcg agcagcttga gctgcaggcg
      841 cagccggcgg acctcaccag gctcgccggt ggtcgttctc cgcggggccc gattgacact
      901 ggatttgaag tgagcgttgc ccagaccccg ccggccaccc ctggcgacga gcagttcctg
      961 accggcctcg gtgagatcgc cgagggtctc cagggtctcg tcgtcgatca ccgaggttcc
     1021 taccggaatc cgcaccagcc tggcgtcacc atgagccccg gtcttgttgc gcccgctgcc
     1081 cggctgtccg ttaccagctc ggtacagcgg ctggtagcgg aaatcgacca gggtgttgag
     1141 cgagtcgtcg gcaaccagaa tgacgtcgcc gccatctccg ccgtcaccgc cgtcgggccc
     1201 tccgcgggcc acgtacttct ccctgcggaa gctcaggcag cccccgccgc cctttcctgc
     1261 ttccaccctg atcgtggctt cgtcgatgaa ctgcatggcg tgctcaacgc aaaaaaaacc
     1321 ccgcgtccgc ggggtctggc tggttcaggc ttccgccgtc gccggctcca cgcagacgaa
     1381 tttgcgcttg tgcggacccc gggtgagaaa ttgaacgtag ccgtccgcgg ttgcaaagag
     1441 cgtgtgatcg cgcccgcagc ccacgttgtc gcccgggtga aacttggtgc cccgctgccg
     1501 aacgatgatg tttcccgccg caacgaactg gccgccatac ttcttgacgc caagccgttt
     1561 cgactcggaa tcgcgaccgt tccgggtgct gccgccagct ttcttgtgag ccatttcctg
     1621 cctcccgccg tttagccgtt tatgcccgtg atcttgagct cggtgtacca ctgccggtgg
     1681 ccctgacgac gcatgtagtc cttgcgccgc ttgaacttga tcacccttat cttcttgccg
     1741 cggccgctgg acacaacctc ggcggtgact ttacctccgt cgacgaacgg ttggcccacg
     1801 gacacatcgt caccgtcggt gaccatgagg actttgtcga acaccacctc gtcgcccggt
     1861 tcacctgcca gccgctccac tcgtatgacc tctccttcgg tgacccggtg ctgcttgcca
     1921 ccgctggaga aaaccgcaaa catacctaaa ctcctctatc caggcggcag cacgatgccg
     1981 cgacgaacac ccacccgagg gctataattc gagccttgct gaagcgattg tcccgggctt
     2041 aagctggcgg cccgtcgcgt aaaatgctcc gggacggcgc cgaaaggcgc gtaattctac
     2101 gggcaaggat attgaattac aacgagttag ccgatgccag gtgacccggc catagcaacc
     2161 acaacgacgc gaatcagcgc cgacccgaac gacgcgctga cgcccgtgta cgacctcgcg
     2221 agggacgatt tcgcggaggt caatcgcctc attccccacc agctgacttc cgacgtggag
     2281 ctcgtcgagg agatcggccg ctacatagtg gacagcggag gcaaacgcct ccgtccgctg
     2341 atggtgctgc tgggcgcccg ctgctgcggc tacgagggcg atgaccacgt gcgccttgcc
     2401 gccatcatcg agttcctcca caccgcgacc cttctccacg acgacgtggt ggaccgctcc
     2461 gagctgcgcc ggggccgggc aacggccaac gcgctctggg gcaatgcccc gagcgtgctc
     2521 gtcggcgatt ttctctactc ccgggctttt cagctgatgg tcgagctgga agacatggac
     2581 atcatggcga tcctagcgga cgcgaccaac accatcgcag cgggcgaggt gcagcagttc
     2641 gcgagcatcg gcaacttcgc aatcgacgaa gcccagtaca tggagatcat ccgctgcaag
     2701 acggcgctgc tgtttcaggc cgccacccat acggccggcg tgctgagcag cgaagatccc
     2761 gacgaagtcg ccgctctcaa aaatttcggc ctccatttcg ggctcgccta ccagctggtc
     2821 gacgactggc tcgattacga gggcgactcc tccaccctgg gcaagaacgt cggcgacgac
     2881 ctggccgagg gcaagctgac cctgccgctg attcatgcgc tggcaaacgc accggacgcc
     2941 gacgccaatg tcgttcgcga cgccgtgagc gcccgatccg ccgagcgtct cgccgaagtg
     3001 cttcaggtcg tgcgttcctc cggcgcgttg agctacaccc gcagcgcggc aacccggcaa
     3061 tgcgcgcggg ccgtggagtg cctaacggga cttcccgaca acgagtaccg cgacgcgctg
     3121 gagacgctgg cgaacttctg cctggatcga ctccgctagc ggtgcgtccc gcaccctcga
     3181 ttctgctggc ctgcctgtgt ttcttgccct tcgccagcgc gctggcggcc gcgcccgcgc
     3241 tcgagcaatg cgagctcagc ggcagccatg gggtcggccg cgtggaggcc gtgtgcggcc
     3301 ggctgcctcg acccgagaac cccgaacagc ccgacggagc tcggctcgac cttttcatcg
     3361 cgaagatacc ctccctgagc ccggaacccg caccggatgc gttcaccatc atcaacggcg
     3421 gtcccggggc ttcctcggtg tcgctgttcg tcgacctcgc ccaggcgttc tcgggcgttc
     3481 tgcgcgagcg ggacatcgtt ctggtcgacc aaagaggcac cgggcggtcc aacgccctcg
     3541 aatgcccgga gctggagaag gccgccgagg actactccat cgaggccgtc caggaagcaa
     3601 cacgggcatg ccttgagtcc ctcgccggcg acccccgttt ctataccacc tcccaggccg
     3661 tgcaggatct cgaggcggtg cgggcagcgc tgggatacga aaggctcaac gtctatggcg
     3721 tgtcctacgg aacccgggtt tccatgcact atctgcgtcg ttatccggac cgggtcagaa
     3781 cgttgatcct ggacggcgtg gtgcctcccg ggctggctct cggcgtcaac gtggccgaga
     3841 acgcgcagcg gcgcctggac gagctgttcg cccagtgcga ccgggcaccg gagtgcagcg
     3901 aggcgtttcc tgaccttcag gccagtttca cccagctgca gagcaatctg aacgccgagc
     3961 cggtcagctt gcggttcccc aatccgataa ccggcctgga agaaagcctg gcgttcggct
     4021 acgaccatct cgccctggtg gtgcgcctgt tgagctacgc cccggaaacc gcatcgcttt
     4081 tgcccctgat catcgaccag gcggctggcg gcaactatct gccggcggcg tcccaggcac
     4141 tgcgaatcac ggacgcgctg agcggcacca tgagcttcgg catgcacaac tcggtcacct
     4201 gcaccgagga tctgcccttc atcgatttca gccaggtgaa ctgggatgct ctggaggcga
     4261 cctatatcgg cgccgatcag gtccgggccc ttcgcgcgat ctgcgagatc tggccgcgcg
     4321 gggcaatgga tccggacctg aaggatcccg ttcaggccga cacgcccacg ctcatcctgt
     4381 ccggcgagct ggaccccatc acaccgcccg catacgggga ccagataaag ccgggcttcg
     4441 gcaactcgat gcacatcatt ggtgcgggtc agggtcacgg cgtcgtatcc aggggatgtc
     4501 tgccgggtct cgtcacgcag atggtggagg cagggcacct gggggacctg gacgccggct
     4561 gcgtcgagcg cctgtccgcg gcaccattct tcatcgatct gatgggtcca gccccatgat
     4621 ccgcgccgag gggctcgaca aatcgtttgg cgaggtcacc gccattcgtc acgtctcgtt
     4681 cgatgcggcc gacggcaaag tcactgccct gctcggcccc aacggcgccg ggaagtccac
     4741 ctcgctcagg attctggcca ccgtgctgca acccgatggg gggaaagccg aggtcgacgg
     4801 cgtcgacgtc gtcgccgacc ccatggcggt gcggcgtcag atcggcgtgc tgccgcacgc
     4861 cgcagggctg tacgcccgcc tcacggcccg ggagaacgtc gcgtacttcg ggtctctgca
     4921 cggcatcgcg aagtcacggc tgagcgaacg catcgatgag ctgatcgagc ttttagaaat
     4981 gggcgagttc gccgaacgcc gggtcgccgg tttttcccag ggtcagcgga tcaaggtcgc
     5041 tcttgcccgc gcgctcgtcc acgacccgcg caacgtcctg ctggacgagc ccaccaacgg
     5101 gctcgacgtc atggcaacgc gggcgctccg agacatcatc atcaggcttc gcgacgaggg
     5161 gcgctgcgtc ctgttctcga gccacatcat gcaggaggtc gccaacgtct gtgacgacgt
     5221 cattgtcatc ggccatggac gcgtgctttt ccaggggacc ctggacgggc tccgcgacca
     5281 cgccggcgaa gccgatctgg aggaggcctt catccgcgtg atcgacgagg aggacgtgtg
     5341 accggtttcg ccatcgtcct tgccaaggaa tgccgcgaca acattcgcga ccgtcgaacg
     5401 atcatctcgt cgctgtctct cgccgtcctg ggacccgcgc tgttcgtcgg gctgatggcc
     5461 ttcgttctgg actccgcggt gggagagtcc agcgatccgg tggagatcac cgtcgtcggc
     5521 gccgaccacg cgcccgggct gatggccttc ctggaaaccc agaacacgcg catcgagcgg
     5581 gtagatctcg aagacccccg gggtgcggtg gccggcggat cccgaaagct ggttctgtcg
     5641 attcccgatg attattccga gcgcttcgcg gcgggccagg tgaccaccgt gtccctcatc
     5701 tacgacagct cggacatggg catgtcccgc cgcaactttc ggatggcgca gcagttcatc
     5761 ctggcgcacg ccaggatgat cgggcttctc cggctccagc ttcgcggggt cgacccggcc
     5821 atcgcgtccc ccgttcaggt tcaggcagtc gacacggcct cgccggccgc ccgcgcgctg
     5881 gccatcctgg ccacctggcc ctacctcctg gtgctcgtca tcttcatggg cggcttttac
     5941 ctggccatcg acgcaacggc cggcgagcgc gagcacggct ccctggagcc gttgctgacc
     6001 cagcccatat cgcgcggcgc cctcgtgctg ggcaaggtag ccgccacggc ggtgttcagc
     6061 gcagcctccc tggcgttgtt cctcgcaagc ctggccatct ctctgcccta cgcgcccctc
     6121 cacaaggtgg gcatgtcgct gcacatcgat gtctcgaccg cgttgaccgt tttcgtcatg
     6181 gcgctgccgc tgatactgtt cgcggccgcc ctgctgaacg tggtcgcttc gttcgcgaag
     6241 agctacaagg aagcgcagac ttacctgacg ttcgtcatcc tcatccccac gcttcccctg
     6301 atcatcacgc gaatgatgaa cgtggagcct tccgcggccc tgatgatgat tccgagcctg
     6361 agccagggga cgttgatcaa cgacgcggtg gcgggtgaac ccgtgcaatg ggtttttctg
     6421 ctgatatccg tggtctcgac gacgctgacg gccggggcgc tgacgatgct ggccgtgtac
     6481 ctttacaagc gcgaacgcat attgatctga tgttgttccc gcgccggggc gcttttacaa
     6541 tacgcggccc tcaccagggg cagtcggagt gtagctcagc ttggtagagc accacgttcg
     6601 ggacgtgggg gccggaggtt caaatcctct cactccgacc attttgactc agtggtttgc
     6661 ggctaccgac gcaccaccga cgcgtcccgc tcaaagcgcg acgcaagccc cgcgccggcc
     6721 ggttacaatg cccgataccg catcatgcct ggagcccaag acatgccgga cgacgaactc
     6781 aaacgcgcgc gcgacgacgc ccgctgcgag gcggcaatcc agaccctcac agacgtgctg
     6841 tggcggttcg aggaaacctg cgagggctcc tacaccctct acgacctcgt gggctacctg
     6901 gtggaagacc tggtccggga gggctgctgc gccgcgtgca tcgcggagtc cgtgacggcg
     6961 gcgttcaagg acatcggcgc cgataccacc gagcaccggc gcgacgaggg aaccatacac
     7021 tgaccaccgc ctgcctggcc tgccacattc tttgtcgcta ggattgtgct cccgtcaggg
     7081 caggagggtt ttcgcgtgag tctgttccgg ctcgacaaca agaacatcgc catcactggc
     7141 gcatcctcgg gattcggcca ccacttcgca ggcgtgcttt ccgaggccgg ggccagcgtg
     7201 gtgctcggcg cccgccgcga agacaagatt cgcgaccggg tcgacgagat caacgctgcc
     7261 ggcggcaagg caaccgcggt aaagctggac gtgcgagacg ccgacagcgt cagaggcttc
     7321 ctcgaagccg ccgaggctgc ctacgggccc ctggacgtgg tcatcaacaa cgccggcgtc
     7381 gaggctggcg cgaaaaccta tctgatgatc gacgaggatg actgggactt cgtcatggat
     7441 acgaacctca aaggtgcgtg gctcgtctcc aagacctaca ccgagctcgt cctcgcggcc
     7501 ggtcgcaagc agggcaacat catcaacatc tcatccatta ccgatacccg cacgataaag
     7561 ggacagtttc cgtacgccgt ctccaaggga gcgctcaccc gcatgaccga ggtcatggcc
     7621 ctggaggcgg ccaagtacgg gatacgcgtc aacgcgctcg cgcccggcta catcctcacg
     7681 gacgtgagcc gcctgctgct cgagagcgat caggcgcccg agttcgtgaa gggcattccc
     7741 atgcgccggt tcggcgagtt ctcggacctg gacggtcccc tgttgctgct ggcctccgac
     7801 gcttcgacct acatgacggg atccgtggtc gtcgtggacg gcggccacat ctgcgcgtcg
     7861 ctgtgacgct gaaaccaggc aggagacaga ccatgaccga cgtacgcccc ttcaggatcg
     7921 agatacccga gaaggatctg gacgacctgg gccagcgcct ggcgctgacc cggctgcccg
     7981 agaaggaaac gcccgacgac tggagccagg gagtgccgct ggcgtacatg cgcgagatcg
     8041 tcgactactg gcagtccgat tacgactggc gacgcgccga ggaagagatc aatcgcttcc
     8101 cgcagttcac caccgagctc gacgggctga acgtccactt catccacgtg aaatcgcccg
     8161 agccgaacgc cctgcccctg gtaatgaccc acggctggcc cggctccatc gtcgagttcc
     8221 tgaaagtgat cggcccgttg gccgatcccg ccagtcacgg cggcgatccg gcagacgcct
     8281 ttcacgtggt ctgcccggcg ctccccggct acggcttttc gggcaagccc atcaccccgg
     8341 gctggggggt ggagaagatc ggcagcgagt gggcaaacct aatggcccgc ctgggctacg
     8401 aaagctacgt cgcccagggg ggcgactggg gctcggcagt caccaccgct atcgggatgc
     8461 aggatcccga ccactgccgc gctattcacc tcaacatggt ggtcgcccag ccggatccgg
     8521 caaccatgga cgacctgacc gacctcgaga aaagaggcat agcgggcatg cagcactacc
     8581 agcagtggga ctccggctac tccaaggagc agagcacgcg tccccagacc atcggctaca
     8641 gcctggtcga ctcacctgtg ggccaggccg cctggatcat ggagaagttc tgggcgtgga
     8701 ccgattgcga cgggcatccg gagaacgccc tgagccggga cgagatgctc gacaacatca
     8761 tgctctactg gctcacggcc agcggcgcat cgtccgcacg gctctactgg gagagcttcg
     8821 gcagcttctc cgcaggcgac gtgagcgtgc cgacgggatg cagcatcttc ccgaaagaga
     8881 tcttcacctg ctcgcgccgg tgggcggagc agcgcttcaa gaacatcgtc tactggaacg
     8941 agctggaccg gggcggacac ttcgccgcct tcgagcagcc tgagctgttc gtcggcgagc
     9001 tgagaaactg cttccgccag gtacgcgggt gaccgagggg cccatctggc gcccggatcc
     9061 ggtccgggcg ctggaatcga acatggcccg cttcaccgag gaagccgccc gggtatccgg
     9121 ccgctccatg acgggctatg cggacctgca cgcctggtcg atcgaagagc ccgccgcctt
     9181 ctgggatctg gtctggcatt tcacggacgt gcgagcttcc caagcgccaa ccgcgatcct
     9241 gaccaatgcg gatcagctgc cgggagcccg atggttcgag ggcgcgaggc tgaacttcgc
     9301 cgaaaatctc ctgcgcttcg acgatgatcg cccggccctg atagcgcttt tggagaccgg
     9361 cgagcggcgt gagatttcct acgccgagct gcgccgcgag acggcacagg tcgccgcgtg
     9421 gctcaaaaat cacatcgacc cgggagaccg ggttgccggg tggctgccaa acgtgccgga
     9481 ggcggtgacc gccatgctgg ccaccacctc cctgggcggc gtctggtcgt cgtgctcgcc
     9541 cgacttcggc gccgagggc
//
LOCUS       DBNHQR010000376         2740 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_478474, whole genome shotgun sequence.
ACCESSION   DBNHQR010000376 DBNHQR010000000
VERSION     DBNHQR010000376.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_478474"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="AC16VR_08520"
     CDS             <1..670
                     /locus_tag="AC16VR_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="Rossmann-fold NAD(P)-binding domain-containing
                     protein"
                     /protein_id="HIR6011238.1"
                     /translation="KETAASTYVDAHERTSEVLCQAADSAGALRIVYLSIVGSEPGSS
                     NPCLASKGAAEAILLAAATPALVVRVPMVLGEGDYAAGALARRARKGWNVLLRGASLE
                     QPIYAGDVIEAVKAGLGSAPAPAALDDAVLDLAGPVSLSRSDLTRRSASILGRSTRVI
                     SLPLFLGLLLAGLLERLLANPPVTAAMLRVLDHDDRLDPEPACHVLGIELTPLDEVLA
                     RCLR"
     gene            803..2494
                     /locus_tag="AC16VR_08525"
     CDS             803..2494
                     /locus_tag="AC16VR_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011239.1"
                     /translation="MQELSNSGLTRSADNAPEETGSGPARAPGKGRELDDLKQELAKW
                     QERVPKLAAALRERTEHASELQAQIEKLEANHRASSDQAAAASKERQQRVDELHSQLQ
                     AQQAKHQELQSELQARGAQIADLRLEVADWKEKHQHLVEAGEQASGPSEEDQAALEAL
                     KRENASLAEALAEKTRLAEERAREVASLREDAESLSSRNATLFETTEFASKQLDSLGD
                     NLGELRDELNARSEALREKTARIEALEGQLSERETELEVFREKMESAGVYEEMALELE
                     ESLAAERQEVERLQQCVKTANRITSEREYERRALSEELETLMERNQELEAAAEAGGDQ
                     DPELEQQRDLMLSLEEELAQAKDEQVALEKSHAKTLAEKERQLSELREELDAAADRAG
                     KLAADESIGEAQPEHPAQDKKVEVLQQQVTTLEELIRERTEELNELKWRAQLADGERE
                     AITEDDAKMMLVLNQQLGEARESNDRLIAVIGRLEERLAASDLGPAGDDFTRMRGIGD
                     KLAAQLNELGIRTFGQIADIDLAALENDHHPLAAFKHRIEKDDWVAQARELAGRG"
BASE COUNT          520 a          860 c          943 g          417 t
ORIGIN      
        1 caaggagacg gcggccagca cctacgtcga tgctcatgag cgcacctccg aggtgctctg
       61 ccaggcggcc gactcggcgg gcgccttgcg gatcgtgtac ctgagcatcg tcggctcgga
      121 accgggcagc agcaatccgt gtctggcgtc gaagggggct gcggaagcca tcctcttggc
      181 cgccgccacg cccgcgctgg tggtgagggt gcccatggtg ctgggcgagg gtgactacgc
      241 cgccggcgcg ctggcgcggc gcgcgcgaaa gggttggaac gttctgctca ggggcgcgag
      301 cctggagcag cccatctacg ccggagacgt catcgaggcg gtgaaggccg ggctcggttc
      361 tgcccccgct ccggcggccc tggacgatgc cgtgctggac ctggccgggc ccgtttccct
      421 ctcgcgcagc gatcttacgc gccggtccgc gagcatcctg ggtcgatcca cccgggtgat
      481 atccctgccc ctgttcttgg ggctgctgct tgccggtctg ctggagcgcc tgctcgcgaa
      541 cccgccggtc accgctgcca tgctgcgggt tctcgaccat gacgaccgct tggatccgga
      601 gcccgcctgc cacgtcctcg gcatcgagct cacgccgctc gacgaggtcc tcgcccggtg
      661 tctgcgatga cgaaaccgtg acgcccgtca cggccatctg aattcccacc cagttcacat
      721 tcaggctggg cgaattcaag tagttttgcg cggtgacccg agaaagtcgg ccagacggtc
      781 ctcggagtgg atggataccc gcatgcagga attgtcgaac agcggcctga ctcggtcagc
      841 cgataatgct ccggaggaga ccggttcggg gccggctcgt gctccaggga agggacgcga
      901 gctggacgat ctcaagcagg aactcgccaa gtggcaggag cgggtgccta agctcgccgc
      961 cgcgctgcgc gagcgcacgg agcacgcaag cgaactgcag gcccagatcg agaagctcga
     1021 ggccaaccac cgtgcctcgt cggatcaggc tgccgctgcc agcaaagagc gtcagcagcg
     1081 ggtggacgag ctgcacagcc agcttcaggc tcaacaggca aagcaccagg agctgcagag
     1141 cgagctgcag gcgcgtggtg cgcagatcgc cgacctccgg ctggaggtgg ccgactggaa
     1201 agaaaaacac cagcacctcg tcgaagcggg cgagcaggcg tccgggccct cggaagagga
     1261 ccaggcggcg ctcgaggcgc tcaagcgtga gaacgcgagc ctcgccgaag cgctggccga
     1321 gaagacgcgg ctcgccgaag agcgcgcgcg ggaggtcgcc tcgcttcgcg aggacgccga
     1381 atcgctctcg agccgcaacg cgacgctctt cgagaccacc gagtttgcca gcaagcagct
     1441 cgacagcctc ggcgacaacc tgggcgagct gcgcgacgag ctcaacgctc gcagcgaggc
     1501 gcttcgggaa aaaaccgctc gaatcgaggc cctggaagga cagctcagcg agcgtgaaac
     1561 cgagctcgag gtcttccggg agaaaatgga gtcagcgggc gtttacgagg aaatggcgct
     1621 ggagctcgaa gagtcgctgg ccgccgagcg tcaggaagtc gagcgcctgc agcagtgcgt
     1681 caagacggcc aaccgcatca ccagcgagcg cgagtacgag cgtcgggcgc tgtccgagga
     1741 gctcgagacg ctcatggaac gcaatcagga actggaggct gcagccgaag cgggcggcga
     1801 tcaggatcct gagctggagc agcagcgcga tctgatgctc agcctggagg aagagcttgc
     1861 ccaggcgaaa gacgaacagg tcgccctgga aaaatctcat gcgaagaccc tggccgagaa
     1921 agagcggcag ctctccgagc tcagggaaga gctggatgcg gccgccgatc gtgccggcaa
     1981 actcgccgct gacgagtcga ttggggaagc acaacccgaa cacccggccc aggacaagaa
     2041 agtcgaggtg ctgcagcagc aggtaaccac gctggaggag ctcatcagag agcgaaccga
     2101 ggagctcaac gagctcaagt ggcgggctca gctcgctgac ggcgagcgcg aggcgatcac
     2161 cgaggacgac gccaagatga tgctggtgct gaaccagcag ctcggcgaag ctcgtgagag
     2221 caacgaccgc ctcatagccg tcatcggtcg tttagaggag cggctcgccg caagcgatct
     2281 gggtcccgcc ggtgacgact tcacgcgaat gcgcggtatc ggcgacaagc tggccgcgca
     2341 gctcaacgag ctgggcatac gcaccttcgg gcagattgcc gacattgatc ttgccgctct
     2401 cgagaacgac caccatcccc tggcggcgtt caagcatcga atcgaaaagg acgactgggt
     2461 cgctcaggcc cgcgagcttg ccgggagagg ctgatggcct cttgagcggc tcgtcgccgt
     2521 gggtccctcg cgggcaaagg acccggcttg ctcaccttga gttttccgcg ggtaactcgc
     2581 tatagtctgc tgcgctcgcc gccctttcga ttgcagctac caggaggttt catgtcgcac
     2641 tcatccgtgc cgttggtttt gcgccgtacg acgagttttc tgttcatggt cgttctggtc
     2701 ccgatggggc tgggtgtggg ctcggcggtc gccgacgtcg
//
LOCUS       DBNHQR010000377         1880 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_478594, whole genome shotgun sequence.
ACCESSION   DBNHQR010000377 DBNHQR010000000
VERSION     DBNHQR010000377.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_478594"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="AC16VR_08530"
     CDS             <1..758
                     /locus_tag="AC16VR_08530"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6011240.1"
                     /translation="QIDLSNVPRSIEEMTRMRERGSRAFAIPESPAGKPRFARNGEPA
                     LAKSLTHPDFEPLWSAAEDLGMAAIAHVGFSRERINPGWANNGADDLMTYSFLNMVVN
                     PQLAPQLLLAALAFDGVFERHPKLTVIVEEVGIAWLPPLLDALDQGVGRAGHRALEDG
                     AYRPDFAGSAYNLPLSPSEYLRRQVRVTPLVASQPLHPTIDLVPEMLCFSSDYPHVEG
                     SGEAMAVCERQLGTLSDEAHRAFYAGVGEMIGL"
     gene            864..1541
                     /locus_tag="AC16VR_08535"
     CDS             864..1541
                     /locus_tag="AC16VR_08535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HIR6011241.1"
                     /translation="MEYDEVVLGRRSIRGFSKDPVPKALIREVVEIAMRAPSSLNTQP
                     WHFHVVSGEPLDRIREGNTERNLAGVPDSREFRGHGGYKGEHRERQKQIAGQLFEAMG
                     IDWGDKPRRQDWVLRGFRQFDAPVSVVVTYDRSIHGGDIAPFDCGAVTNALVNAAWSR
                     GLGCVINSQGIMQSPVVREHARIPDDEVIMICVAMGFPDDSFPANAVVSERKPVDEAV
                     SFVGFQD"
     gene            complement(1538..>1880)
                     /locus_tag="AC16VR_08540"
     CDS             complement(1538..>1880)
                     /locus_tag="AC16VR_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011242.1"
                     /translation="ASRAQIPGKLFEYLPTGRPILALVPGDGATWRLCEALEQVFLVD
                     AGNPGAFAGRIDEFLAAAHDDKRFPVPPEYSEDALAEVFLSSLVTPECAITADDSAGA
                     GAKGLNGKADL"
BASE COUNT          339 a          616 c          600 g          325 t
ORIGIN      
        1 cacagatcga cctgtccaac gtcccgcggt ccatcgagga gatgacgcgc atgcgcgagc
       61 gcggcagccg ggcgttcgcg atcccggagt ctcccgcggg caagccgcga ttcgcacgca
      121 acggcgagcc cgcgctggcc aaatcgctga cccacccgga tttcgaaccg ctgtggtcgg
      181 cggccgagga cctgggcatg gcggccatcg cccacgtcgg attttcccgg gaacgcatca
      241 accccggttg ggccaacaac ggtgccgacg atctgatgac ctacagcttt ctgaacatgg
      301 tggtcaaccc ccagctcgct ccgcagctgc tgctggccgc tctggccttc gatggcgtgt
      361 tcgagcgaca ccccaagctt actgtgatcg tggaggaagt cggcatcgcc tggctgccgc
      421 ctctgctcga cgccctggac cagggcgtgg ggcgggccgg tcaccgggcg ctggaagatg
      481 gcgcctaccg gccggacttt gcgggatccg cgtacaacct gccgctctcg ccctccgagt
      541 acctgcgccg acaggttcgc gtgaccccgc tggtggcctc gcaacctcta caccccacca
      601 tcgacctggt tcccgagatg ctctgcttct ccagcgacta tccccatgtg gaaggttccg
      661 gcgaagccat ggcggtgtgc gagcgacagc tgggaaccct gagcgacgag gcccaccggg
      721 ctttctatgc gggggtgggg gagatgatcg ggctctgacg cgccgtcgtc gcagctctga
      781 atgcccgtat gcccagcctg gacgggagct ggtcaacccg gcaaacgagg tagaatccga
      841 gctttctcac tcaggagcca tcgttggaat acgatgaagt cgttctcggc cgccggagca
      901 tccgaggctt cagcaaggat ccggttccca aggcactgat acgcgaggtt gtggaaatcg
      961 cgatgcgagc gccctcctcg ctcaataccc agccctggca ctttcatgtg gtcagcggag
     1021 agccgctgga ccggattcgc gaaggcaata ccgagcggaa ccttgccggc gtgccggatt
     1081 ccagggagtt ccgcgggcac ggcggctaca agggggagca ccgggaacgg cagaaacaga
     1141 tcgcggggca actgttcgaa gccatgggca tcgactgggg cgacaaaccc cgccggcagg
     1201 actgggttct gcgtggattt cgtcagttcg acgccccggt ctcggtcgtg gtgacctacg
     1261 accgatccat ccacggcggc gatatcgcgc ccttcgactg cggagcggtc accaacgcgc
     1321 tcgtcaacgc cgcctggtcc agggggcttg gatgcgtcat caacagccag ggcatcatgc
     1381 agtcccccgt cgttcgcgag cacgcgcgca ttccggacga cgaggtgatc atgatctgcg
     1441 tggccatggg ctttccggac gacagctttc cggccaacgc cgtggtttca gagcgcaaac
     1501 ccgtggacga agccgtttcg ttcgtggggt tccaagacta aagatcagcc ttcccgttga
     1561 gccccttcgc acctgcgcct gcagaatcat ctgcagtgat ggcgcattcc ggcgtcacga
     1621 gcgacgacag gaagacctcc gctaaggcgt cctccgagta ctcgggcggc acggggaatc
     1681 gcttgtcatc gtgggcggca gcaaggaact catcgatcct tccggcaaag gctccggggt
     1741 tgccggcgtc gaccagaaaa acctgttcca gtgcctcgca cagccgccac gtggcaccgt
     1801 caccaggaac gagcgccagg atcggtcgac ccgtgggcaa atactcgaac agtttaccgg
     1861 gtatctgtgc ccgcgatgcg
//
LOCUS       DBNHQR010000378        10170 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_98769, whole genome shotgun sequence.
ACCESSION   DBNHQR010000378 DBNHQR010000000
VERSION     DBNHQR010000378.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10170)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10170)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10170
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_98769"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="AC16VR_08545"
     CDS             <1..360
                     /locus_tag="AC16VR_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HIR6011243.1"
                     /translation="RLGGCPTGEARITGGYDLPAAHVIHTVGPIWRGGDAGEAALLTA
                     CYRNSLDLARRHHIRSIAFPAISTGAFGYPLAEATAIAVATVGSFIATTPLLARFVCF
                     DARTAHAYREELAAQTG"
     gene            complement(348..974)
                     /locus_tag="AC16VR_08550"
     CDS             complement(348..974)
                     /locus_tag="AC16VR_08550"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF020341.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HIR6011244.1"
                     /translation="MDRARALAMIQTMAEMQESHNRHVHPEWIEQGYAYYRAVWVECA
                     ELLDHFGWKWWKKQQPDLGQVKLEVVDIWHFGLSDLIRAGTLDTAAPVLLKVSASEPT
                     DLEGFRLAVEALAADTLSSRAFAMAPFAAVLESLPMSFAELFEMYVGKNVLNNFRQDH
                     GYKTGEYQKLWQGREDNEHLIDILGELDCPAESVPAELYRALQARYPV"
     gene            1035..2042
                     /locus_tag="AC16VR_08555"
     CDS             1035..2042
                     /locus_tag="AC16VR_08555"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HIR6011245.1"
                     /translation="MAKSPSTALVMHPVVRDELITHAHMERLRKTVNFIDEEPFRDLD
                     AIGGALGQVELLITSWGCPRVDADCLARLPRLKLIAHLAGSVKGFLDDIVWRRGILVT
                     NAVAANAVPVAEYTLAAILFANKRVFQLNRVYLEHREHRPPWTKEAPDVGNYHKSVGI
                     VGASHVGRLVMEHLRRFDIRLLLYDPFVTPLAARDLGATKVGLSELLSQSDTVSLHAP
                     LLKETRHMIGARELALMHDGATIVNTARGALIDQAALEAELTRGRLYAVLDTTDPEEL
                     PPESPLYGLPNVFLTPHIAGSLGTETQRLTDCIIEELERYGRGVPLKYRVRREQLDRL
                     A"
     gene            2142..3332
                     /locus_tag="AC16VR_08560"
     CDS             2142..3332
                     /locus_tag="AC16VR_08560"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6011246.1"
                     /translation="MDLSYGPHYEEFREEVRSFLADHAGSAPRGEHIRSEKTLAWQHL
                     LIENGYAARTIPREYGGYGAAPDILESRIIAEEFAAARVSSGLGGQGISMLVPTLLEM
                     GTQEQKTTFIPPTLRGEMLWCQGYSEPGAGSDLASLSTRAELDGDEWVINGQKIWTST
                     AHIADWMFCLVRTEPDAPKHQGISFLLLRMDTPGIEVRPLVDMTGAANFNETFFTDVR
                     VPKDQIVGRRGEGWLVANAILGHERDSLGDPNVMLSRFNALTELMKTETLGGERLIDH
                     PVFRDRLMQIQGRVMALSYNDLRLLSAKVNAADARLARMIVKLQGTELRHDLEGLAVD
                     AMGELGLAYGDNPYLRDHGSWQSQYMFYLGLIIGGGTSQIQKNIISERGLGMPKEPKY
                     EKQA"
     gene            3342..4409
                     /locus_tag="AC16VR_08565"
     CDS             3342..4409
                     /locus_tag="AC16VR_08565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6011247.1"
                     /translation="MEFGLSEEQTLLQDSLNGFLDANCPLDRVRRFAEEPVAADLHSG
                     LAELGVFGLLVPEEAGGVGLGCFEAALVAEALGSHTAPCAFLGSAVMAPLALTLAGNE
                     PTDTLSRLATGELTVGVAVAEFVGARADGGITADGETLSGKALSVLDFDAQAYLVADA
                     AGSTYLVDAGAPGVFAEALPTIDKTRRTGALTLNGAPAQLICDDPDVLARVIDAGRVM
                     LAADTLGAAQNMLDQAVEYAMQREQFNRPIGSFQAVKHMCAEMAASLEPCRAMVWYAS
                     HALDDLPAEASLCACHAKAHLSEVGKFVAKTATEVHGGMGFTDLLGLHYWFKRIGYNR
                     QMLGAPELLREQAARLQGLCA"
     gene            4420..6747
                     /gene="lon"
                     /locus_tag="AC16VR_08570"
     CDS             4420..6747
                     /gene="lon"
                     /locus_tag="AC16VR_08570"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIR6011248.1"
                     /translation="MPVLPVLPLKDTVVYPQIVRPLGVGRRRSLAALNHAVEGPREII
                     TVAQHDGANEEPGLTDLYAVGCIASISRVENRDGGAQVIVQGTRRVRLTGIAEDEGAF
                     LAAQYEELPGLGMNDLGTDGPAVDATLRENLQLAQRIAEIMDSENGAQIYQQLVGSIS
                     DPITQMYRIASLAPGIELIQEQRILEADTTLALMEAIHELLGHELQVTELRRQIAERA
                     RDDMDQQQREHVLRQQKRAIEQALGESGEDDDIAELKGQLDEARLPEPVQAEVDRELK
                     RLARMSANAADYQVARAYLELIGELPWHQETEDQLDLKHAREILDEDHYGLEDVKERI
                     LESLAVMQLNPGAKAPILCFVGPPGVGKTSLGQSIARAMGRKFERLSLGGLHDEAELR
                     GHRRTYIGAMPGRILQGVRRAGVTNPILMLDEVDKLGRDFRGDPAAALMEILDPAQNR
                     EFRDNYLNLPYDLSRVLFITTANTLEGIPRPLLDRMEVLDLTGYSDLEKQAIARQYLI
                     PRQRSEAGLSEAQLDISDDALTTMIRRYTREAGVRGLERVIGRLARKRARQVVDGQSL
                     DTPVAADDLAELLGPERFKSDRGRTGLEPGVAAGLAWTEAGGDVLYVEATLTQKDEKV
                     TLTGHLGQVMQESARAARSHLWSAADRLGLDRPAIEESGVHIHVPAGAVPKDGPSAGV
                     TMACALYSAYKGRALRDDVAMTGELTLSGLVLPVGGVKEKILAAHRAGLRRVILPKDN
                     EADLAKLPQTVLDEMQISLAAHLDDVLAVAFAEES"
     gene            complement(6744..8561)
                     /locus_tag="AC16VR_08575"
     CDS             complement(6744..8561)
                     /locus_tag="AC16VR_08575"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HIR6011249.1"
                     /translation="MALESVIEGPAWDLTGEYSSPDSQGLQADLDRLSELLDRIEALN
                     GQLNGSGAVAAAQQIHRLADQARELLADPRTYASCLLSVNGRDEAALMLQGRLQGYQT
                     RFGDLLKPLELFIDLADDEGIEAFLADPEVAPAEFVVRHARKRRHERLGLAEEQLING
                     LSQEGIHAWGNLYDQLSGTLTCEVLVGNETREMGIAEAAGLLQKPDVATRRNAWRAIN
                     DSWSAHAETCAASLNAIAGWRLELGRRRGREAPVHFLDAPVHMNRISRETLDTLMDVA
                     AGSRPLARRAARLMARAYGLETIGPWDQRAPAPDLGTAPAAGIPYDQALHTIADAYAD
                     VHPSMGEFVRMMHANRWIEGPRGPNKRPGAYCTGFAKSRTPRIYMTYSGGSSDVITLA
                     HELGHALHSWVMRDLPNSQRGYGMALAETASTFGETLVRDALLGSAGSPQAKLDMAWE
                     EMAALVSFVLNIPTRFEFERRFYEARRERPVLPRELNAMMADAWTECFGDVLEEPDPM
                     FWASKLHFYISGISFYNFPYLFGYLFSLGVYARRGDGADFYPRYEGLLRDTGRMTAEA
                     LAAKHLSEDLSRPGFWEATVASLETRVDAFEDLLDELAL"
     gene            complement(8561..9238)
                     /locus_tag="AC16VR_08580"
     CDS             complement(8561..9238)
                     /locus_tag="AC16VR_08580"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6011250.1"
                     /translation="MIELYTASTPNGWKASVTLEELGLPYEVRPVDLMAGEQKAPEFL
                     KINPNGRVPAIVDDGFPIFESGAIMIHLAEKTGRLMPADAHGRSLVIQWLMFQMGGVG
                     PMMGQANVFYRAMEEKIQPAIDRYQNECRRLFEVLDGRLRNSEWLADHFSIADIANWC
                     WARLYYWSGVSLDGLDAMQRWMAAMEARPACQRGVEVPNRLELPRDDDPDGIERQALQ
                     SRRVLVR"
     gene            complement(9294..9989)
                     /locus_tag="AC16VR_08585"
     CDS             complement(9294..9989)
                     /locus_tag="AC16VR_08585"
                     /inference="COORDINATES: protein motif:HMM:NF053177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF8091 domain-containing protein"
                     /protein_id="HIR6011251.1"
                     /translation="MSIGTLNEGPLHQAIKSLYNGAGAREEVPVGDFVADVFGPDEVI
                     YEIQTGGFGSLKKKLARLVEDHRVVLVHPIAEVRYIVKLPQGEDQQASRRRSPKRGSV
                     AHVLDRLVSIPALLNHPNFELEVLLIEEEELRTFDPRRVRRRDGWRVVQRRLGSVLAS
                     ERFTCAGDLLRMVPGPLPAEFTTEDLAGAMGQPRWLAQKLAYCLREAGEISISGKRGN
                     ALCYRLARSGAVA"
BASE COUNT         1829 a         3411 c         3310 g         1620 t
ORIGIN      
        1 aggctcggcg gatgccccac cggagaagca cgaatcacgg gaggctacga cttgcccgcc
       61 gcccacgtca ttcacacggt agggcccatc tggcgcgggg gtgacgccgg ggaggcggcg
      121 ctgctgaccg cctgctaccg aaattccctg gatctggcca ggcgccacca catccgcagc
      181 atcgccttcc cggccatcag caccggcgcg ttcggctacc cgctggcgga agccaccgcc
      241 atcgccgtcg ccacggtcgg cagcttcatc gccaccacac ccctgcttgc ccggttcgtc
      301 tgcttcgacg cccgaacggc tcacgcctac cgcgaggagc tcgccgctca gacggggtag
      361 cgggcttgca gagctcgata gagctcagca ggaaccgact ccgcggggca atccagctca
      421 cccagaatgt cgatcaggtg ctcgttgtcc tcccggccct gccagagttt ttgatactcc
      481 ccggtcttgt agccgtgatc ctgccggaag ttgttgagca cgttcttgcc gacgtacatc
      541 tcgaaaagct ccgcgaagga catcggcagg ctttcgagca ccgcggcaaa cggcgccatg
      601 gcgaaagcac ggctggaaag cgtgtcggcc gcgagcgcct ccaccgccag gcggaacccc
      661 tcgagatccg tcggctcgct cgccgatact ttcagcagca ccggcgctgc cgtatccagg
      721 gtgcccgccc gaatcagatc gctcaggccg aagtgccaga tgtccaccac ctcgagtttc
      781 acctggccca gatcgggctg ctgcttcttc caccatttcc atccgaagtg gtcgagcagc
      841 tcggcgcact ccacccaaac ggcccggtaa taggcataac cctgctcgat ccattccggg
      901 tgcacatggc gattgtggct ctcctgcatc tccgccatgg tctggatcat cgccagcgcg
      961 cgcgctctgt ccattttcaa accgatcctc gcgggtaaac gaaagacaga aaattatggg
     1021 gtattcttca ccctatggcc aaaagcccga gcaccgcgct ggttatgcat cccgtcgtcc
     1081 gcgacgagct gatcacgcac gctcacatgg agcggttgcg caagacggtc aacttcatcg
     1141 acgaggaacc gttccgcgac ctggacgcca ttgggggcgc gctcggccag gtagagctcc
     1201 tgatcaccag ctggggctgc ccccgggtcg atgcggactg cctcgcacgg cttccacgcc
     1261 tgaagctgat cgcccatctc gccggcagcg tgaagggctt tctcgacgac atcgtctggc
     1321 gccgcggcat tctggtgacc aatgcggtgg cagccaatgc ggtgccggtg gccgagtaca
     1381 ccctggccgc catcctcttc gcgaacaagc gggtgtttca gctcaatcgc gtctatctcg
     1441 agcatcggga gcaccgccca ccttggacga aggaggcgcc ggacgtaggc aactaccaca
     1501 agagcgtcgg catcgtgggc gcttctcacg ttggtaggct tgtgatggag cacctgcggc
     1561 gcttcgacat ccgtctgctg ctctacgatc cgttcgtgac gccccttgcc gcgcgagacc
     1621 tcggcgcgac caaggtgggg ctctccgagc tgctgtcgca atcggacacg gtcagcctgc
     1681 acgcgccgct gttgaaagag acgcgccaca tgatcggggc acgcgagctt gcgctgatgc
     1741 acgacggcgc gaccatcgtg aacaccgcgc gcggcgctct catcgaccag gcggcgctgg
     1801 aggcagagct cacgcgcggc cgcctctacg ccgtgctgga caccaccgat cccgaggagc
     1861 tgccgccgga atcacccctg tacgggttgc cgaacgtctt cctgacgcca cacatcgccg
     1921 gctcgctggg gacggagacc cagcggctca ccgattgcat catcgaggag ctcgaacgct
     1981 acggtcgcgg cgtgccgctc aagtaccggg taaggcggga acagctcgac cgcctggcct
     2041 gaaccccctg cccgaccggc caagttgccg ccccttccgg tagcggttat gttgtcgacc
     2101 gcaccctgtt ttgcgcccct gattccgccc gggagattgg tatggatctt tcctacggtc
     2161 cacattacga agagttccgc gaggaggtca gatcgtttct cgccgaccac gccgggagcg
     2221 ccccgcgcgg cgagcacatc cggtcggaga aaacactcgc ctggcagcac ctgctcatcg
     2281 aaaacggcta cgcggcaaga acgattccac gcgagtacgg gggctacggc gccgcgcccg
     2341 acattctcga atcgcggatc atcgccgagg agttcgccgc cgcgcgcgtg agctccgggc
     2401 ttggcggtca gggcatatcc atgctggtgc cgaccctgct ggagatgggc acgcaggagc
     2461 agaaaaccac cttcatcccg cccacgctgc gcggcgagat gctgtggtgt cagggatatt
     2521 cggaacccgg ggccggcagt gacctcgcca gcctgtccac gcgcgccgag ctcgacggtg
     2581 acgagtgggt gatcaacggg cagaagatct ggaccagcac ggcgcacatc gcggactgga
     2641 tgttctgcct ggtccggacc gagcccgatg cgcccaagca ccaggggatc agctttctcc
     2701 tgctgcgcat ggacaccccc ggcatagagg tccgcccgct ggtggacatg accggcgcgg
     2761 cgaacttcaa cgagacgttc ttcaccgacg tccgggtccc aaaagatcag atcgtcggcc
     2821 gccgcggtga gggctggctg gtcgccaacg ccatcctcgg ccacgagcgg gactctctgg
     2881 gcgacccgaa cgtcatgctc tcccgattta acgcccttac agagctcatg aagacggaga
     2941 ccctcggcgg ggagcggctg atcgaccatc cggtgttccg ggaccgcctc atgcagatcc
     3001 agggtcgggt catggcgctg agctacaacg accttcggct gctgtccgcc aaggtcaacg
     3061 cagcggatgc ccgcctcgca cgaatgatcg tcaagctgca gggcaccgag ctgcgccacg
     3121 acctcgaggg tctggccgtg gacgccatgg gagagctggg gctggcctac ggcgacaacc
     3181 cctacctgcg cgatcacggc agctggcaga gccagtacat gttctacctg gggctgatca
     3241 tcggcggggg cacctcgcag atccagaaga acatcatcag cgaacgcggg ctcggcatgc
     3301 cgaaagaacc caaatacgaa aagcaggctt aggaggccgc catggagttc gggctttccg
     3361 aagagcagac actgttgcag gacagcttga acggcttcct ggacgccaac tgccctctgg
     3421 atagggtacg ccgctttgcc gaggagcctg tggcggccga ccttcattcc ggcctggcgg
     3481 agctcggcgt cttcgggctg ctcgtcccgg aggaagcggg cggggtgggc ctgggctgct
     3541 tcgaggcggc gctggtcgcc gaggcccttg gcagccacac cgccccttgc gcttttctag
     3601 gttccgccgt catggcgccg ctggccctca cgctggcggg caacgagccg acggataccc
     3661 tgtcccgact ggcgacagga gaactcacgg tcggggtcgc cgtggccgag ttcgtcggcg
     3721 cgcgggcgga tggcggcatc accgccgacg gggagacgct ctccggcaaa gcgctctccg
     3781 ttctggattt cgatgcccag gcttacctgg tcgcggacgc ggcagggagc acctacctcg
     3841 tcgacgccgg agcgcccggg gtgttcgccg aagcgcttcc aaccatcgac aagacccgcc
     3901 gcaccggcgc gctcacgctg aacggggcgc cggcacagct gatctgcgac gacccggacg
     3961 tgctcgcccg ggtgatcgat gcgggacggg tcatgctcgc agcggacacc ctcggcgctg
     4021 cccagaacat gctcgatcaa gcggtggaat acgcgatgca gcgagagcag ttcaaccggc
     4081 ccatcggctc gtttcaggcc gtcaagcaca tgtgcgcaga gatggccgcg agcctcgaac
     4141 cgtgccgagc catggtctgg tacgcatccc acgcgctcga cgaccttccc gccgaagcct
     4201 ccctgtgcgc ctgccacgcc aaggcccacc tttcggaggt cggcaagttc gtcgccaaga
     4261 cggcgaccga ggtacacggc ggcatgggct tcacggacct tctcggactc cactactggt
     4321 tcaagcggat cggctacaat cgccagatgc tcggagctcc ggagttgttg cgcgagcagg
     4381 cagcccgcct tcagggcctg tgcgcctgac gagaacgcca tgccagtcct ccccgtcctg
     4441 ccccttaaag acacggtcgt ctacccgcag atcgtccgcc ccctcggcgt gggtcggcgt
     4501 cgctctctgg cggccctgaa ccacgcggtg gaggggccca gggagatcat caccgttgcc
     4561 cagcacgatg gcgccaacga ggagccgggc ctgacggatc tctacgccgt cggctgcatc
     4621 gccagcatca gtcgcgtgga gaatcgcgac ggcggcgccc aggtgatcgt tcaggggacg
     4681 cgccgggtac ggttgaccgg aatcgcggag gacgagggcg cgttcctcgc cgcgcagtac
     4741 gaggagctgc cgggtctcgg catgaacgac ctcgggaccg acggcccggc cgtggacgct
     4801 accctgcggg agaacctcca gctcgcccag cgcatcgcag agatcatgga cagcgagaac
     4861 ggcgcgcaga tctatcagca gctggtgggc tcgatctccg atcccatcac tcagatgtac
     4921 cgcatcgcgt ccctcgcccc ggggatcgag ctgatccagg agcagcgaat tctcgaggcc
     4981 gacaccacgc tcgccctgat ggaagccatc cacgagctgc tgggacacga gctccaggtc
     5041 accgagctgc gcaggcagat cgcggaacgg gcccgggacg acatggatca gcagcagcgt
     5101 gaacacgtcc tgcgccagca gaaacgcgcc atcgagcagg ccctggggga gtcgggggag
     5161 gacgatgaca ttgccgagct gaagggccag ctcgacgagg cccgcctgcc ggagcccgtt
     5221 caggcggaag tggacagaga gctgaagcgg ctcgcccgca tgtcggcgaa cgccgccgac
     5281 taccaggtcg cccgggccta tctggagcta atcggggagc tgccctggca tcaggagacc
     5341 gaggatcagc tcgatctgaa gcacgccagg gaaattctgg acgaagatca ctacggtctg
     5401 gaggacgtca aggagcgcat cctcgaatct ctggccgtca tgcagctgaa ccctggcgcg
     5461 aaagcgccca ttctctgctt cgtaggcccg cccggggtgg gcaagacgtc cctgggccag
     5521 tccattgccc gggccatggg gcgcaagttc gagcggttga gcctcggtgg tctgcacgac
     5581 gaggcggagc tcagaggaca ccggcgcacc tacatcggcg ccatgcccgg ccgcatcctc
     5641 cagggcgtcc ggcgcgccgg cgtcaccaac ccgatcctga tgctcgacga ggtggacaag
     5701 ctcggtcgcg actttcgcgg cgaccccgcc gcggccctga tggagatact cgatcccgcc
     5761 cagaaccgcg agttcaggga caactacctg aatctgcctt acgacctgtc acgcgtcctc
     5821 ttcatcacca cggcaaacac cctggagggg atcccgcggc cgctgctgga ccggatggag
     5881 gtgctggatc tcaccggcta cagcgacctc gagaagcagg ccattgcgcg ccagtacctg
     5941 atcccccgac agcgcagcga ggcgggcctt tccgaggctc agctggatat ttcagacgac
     6001 gcgctcacca ccatgatccg ccgctacacc cgtgaagcgg gcgtgcgggg gctggagcgc
     6061 gtcatcggcc gtctggcgag aaagcgcgcg cgtcaggtgg tggacggcca gagcctggac
     6121 acaccggtcg cagccgacga tctggccgaa ctgctgggac cggagcgatt caaatccgac
     6181 cgcggacgca ccgggctgga gcccggcgtc gcggcggggc tggcctggac cgaggcgggc
     6241 ggggacgtgc tctacgtcga agcgacgctc acccagaagg acgagaaggt cacgctcacc
     6301 gggcatctcg ggcaggtgat gcaggaatct gccagggcgg cccgcagcca cctgtggtca
     6361 gcggcggaca ggctcggcct ggacaggccc gccatcgagg aaagcggtgt gcacatacac
     6421 gttccagccg gtgcggtgcc gaaagatggg ccttccgccg gtgtcaccat ggcgtgcgcg
     6481 ctctactcgg catacaaagg cagggccctt cgcgatgacg tcgccatgac cggagagctg
     6541 acgctgtccg gcctggtgct gcccgtcggc ggggtgaagg agaagatcct cgccgcgcac
     6601 cgcgccgggc tgcgtcgggt catcctgccc aaagacaacg aagccgacct tgccaagctc
     6661 ccgcagacgg ttctggacga gatgcagatc tcgctggccg cccacctcga cgacgtcctg
     6721 gcagtggcct tcgccgagga aagctagagc gccagctcgt cgagcagatc ctcgaaagca
     6781 tcgacccggg tctcgagaga tgcgaccgtg gcctcccaga aaccgggccg cgacaggtcc
     6841 tcggaaagat gcttggccgc gagcgcctcg gccgtcatcc gcccggtgtc ccgaagcagc
     6901 ccttcgtaac gggggtagaa gtcggcaccg tcgccgcgcc gcgcgtagac gcccaggctg
     6961 aacaggtatc cgaacaggta gggaaagttg tagaagctga tgccggatat gtagaagtgc
     7021 agcttgctgg cccagaacat cgggtcgggc tcctcgagca cgtcgccgaa acactctgtc
     7081 cacgcatccg ccatcatggc gttgagctcc cggggcagca cgggccgctc ccggcgggct
     7141 tcatagaaac gccgttcgaa ctcgaaacgg gtggggatgt tgaggacgaa gctcaccagc
     7201 gccgccatct cttcccaggc catgtcgagc tttgcctggg gagagccggc actgccgagg
     7261 agcgcgtccc gaaccagagt ctcaccaaaa gtgcttgccg tctcggccag cgccatcccg
     7321 tagccgcgct ggctgttcgg caggtcccgc atgacccagc tgtgcagggc gtggccaagc
     7381 tcgtgagcca gggtgatgac gtcgctggag ccaccggagt aggtcatgta gatacgcggc
     7441 gtgcgggact tggcgaagcc cgtgcagtag gcgcccggac gcttgttggg gccccggggc
     7501 ccctcgatcc atcggttggc atgcatcatg cggacgaact cgcccatgga gggatgcacg
     7561 tccgcatagg catcggcgat ggtgtgcagc gcctggtcat acgggatccc cgcggcgggg
     7621 gccgtaccaa ggtcgggtgc cggcgcgcgc tggtcccagg gaccgatggt ttccaggccg
     7681 taagcacggg ccatcagccg cgccgcccgc cgggcgaggg gccgcgagcc ggcagccacg
     7741 tccatcaggg tatccagggt ttcgcggctg atccggttca tgtgcaccgg cgcatccagg
     7801 aagtgtacgg gagcttcgcg gccgcgccgg cgcccgagct ccagccgcca accggcgatg
     7861 gcgttgagac tggcagcaca ggtttcggca tgggccgacc acgagtcgtt gatcgcgcgc
     7921 caggcgttcc gccgggtcgc cacgtcgggc ttctgcagca ggccggccgc ttcggcgatg
     7981 cccatctccc gggtctcgtt gcccaccagc acttcgcagg tgagcgtgcc ggagagctga
     8041 tcgtagagat tgccccaggc gtgaatgccc tcctgggaga ggccgttgat gagctgttcc
     8101 tcggccagcc ccagacgctc atggcgccgc ttgcgcgcat ggcgcaccac gaactcggcc
     8161 ggcgccacct ccgggtcggc cagaaacgct tcgatgccct cgtcgtccgc cagatcgatg
     8221 aacagctcca gcggcttgag cagatccccg aagcgcgtct ggtaaccctg caaacgcccc
     8281 tgcagcatga gggcggcttc gtcccggccg ttcaccgaga gcaggcagga ggcgtaagtg
     8341 cgtgggtccg caagcagctc ccgggcctga tcggccagac ggtgaatctg ctgggcggcc
     8401 gcaacagcgc cagagccatt aagctgcccg ttgagcgcct cgatccgatc gagcagctcg
     8461 ctgagtcggt cgaggtcggc ctgcaagccc tgagagtcgg gcgacgagta ctcgccggtc
     8521 agatcccagg ccggcccttc gattaccgat tcgagggcca tcagcgcacc agcacgcggc
     8581 gtgactggag tgcctggcgt tcgatcccgt ccggatcatc gtctcgaggc agctcgagcc
     8641 ggttgggaac ctcaacgcct ctctgacagg cgggacgcgc ctccatggcg gccatccacc
     8701 gctgcatggc gtcgagcccg tcgagggaaa cacccgacca gtagtaaagg cgcgcccagc
     8761 accagttggc gatatcggca atcgagaagt ggtcggccag ccactcgctg ttccgaagac
     8821 ggccatcgag aacttcgaac aagcgccgac actcgttctg gtagcggtcg atggcgggct
     8881 ggatcttttc ctccattgcc cgatagaaga cgttcgcctg acccatcatg gggccgacgc
     8941 cgcccatctg gaacatcagc cactggatga ccagagaacg tccatgagcg tcagccggca
     9001 tgaggcgccc ggttttctca gccaggtgaa tcatgatggc gccggactcg aatatcggaa
     9061 aaccgtcgtc cacgatggcg ggaactctgc cgttcgggtt gatcttcaga aactccgggg
     9121 ccttctgctc gccggccatc aggtcgacgg gccgtacctc gtagggaagg ccgagttctt
     9181 ccaaagtgac ggacgctttc cagccgttgg gcgtagacgc ggtgtaaagc tcgatcaagg
     9241 gtatgctcgc tgatgggcgg gcgaaggagt attcgaggcc cccgggccgc atgtcaagcc
     9301 acggcgcccg aacgcgctag ccggtagcac agggcgttgc cccgcttgcc ggagatgctg
     9361 atctctccgg cctcgcgcag gcagtaagcc agcttctggg caagccagcg cggctgaccc
     9421 atcgcgccgg cgagatcctc ggtggtgaac tccgcaggaa gcggcccggg caccatgcgc
     9481 agaagatcgc cagcacaggt gaaacgctcg gaggcgagca cgctgcccag acggcgctgc
     9541 accacacgcc atccatcccg tcggcggacc ctgcgcggat cgaaggtccg caactcctct
     9601 tcctcgatca gcagaacctc gagctcgaag ttgggatgat tcagaagcgc aggaatgctc
     9661 accagccggt cgagcacgtg ggcaacgctg ccgcgtttgg gcgagcgccg acggctggcc
     9721 tgctggtctt ccccctgggg cagcttgacg atgtagcgca cctctgcgat cggatggacc
     9781 aggacgacgc gatgatcctc caccaggcga gcgagtttct tcttgaggct gccgaaaccg
     9841 ccggtctgaa tctcgtagat gacctcgtcg gggccgaaga cgtcggctac gaagtcgccg
     9901 accggcacct cctcacgggc gccggcgccg ttgtagagcg acttgattgc ctggtgcaga
     9961 ggcccttcgt tgagggtgcc gatgctcacg ggcggccatt aactcaggct ccggaatgtt
    10021 tgtctaccag gcccgctatg ccatactccg agccctcaac tgcaacgact ggagggacgg
    10081 acacatgagc gcagacggca cctggaacac cgtgatgaac acgcccatgg gcgcccagaa
    10141 cggcaccatg accctggcca ccgacggcgg
//
LOCUS       DBNHQR010000379         2030 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_986220, whole genome shotgun sequence.
ACCESSION   DBNHQR010000379 DBNHQR010000000
VERSION     DBNHQR010000379.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_986220"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="AC16VR_08590"
     CDS             <1..784
                     /locus_tag="AC16VR_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR6011252.1"
                     /translation="EGVQVAEIGLDQVVPNRYQPRRDFNEEALAELVASIRSQGVMQP
                     VVVRRRAQGGYELVAGERRWRAAGLAGLDAIPALIRDVSDEQASAMALIENIQREDLN
                     PLEEAHALQRLRDEFGLTQQQVADAVGKSRVAVTNLLRLLNLGPAVRDLLVAGAIEMG
                     HARALLSLDSVVQERAANDVVARGLSVRQTEALVRRLQQGKGPGRPSEAVRDADTAHL
                     ERELSEKIGAPVSISCTAKGRGRLVIRYGSLEELDGILGHIR"
     gene            930..1268
                     /locus_tag="AC16VR_08595"
     CDS             930..1268
                     /locus_tag="AC16VR_08595"
                     /inference="COORDINATES: protein motif:HMM:NF015834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HIR6011253.1"
                     /translation="MPYRTVALQALAGLGVVLAALAWDAREAVAALAATIVSVVPNAY
                     FAWRVSRERAAARILAAEVVKVVATVGMMGLAFATMELRPLGFFATFGVLQLAHVVGG
                     TSLAGKATSR"
     gene            1351..>2030
                     /gene="atpB"
                     /locus_tag="AC16VR_08600"
     CDS             1351..>2030
                     /gene="atpB"
                     /locus_tag="AC16VR_08600"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011064386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIR6011254.1"
                     /translation="MSSGTVTSGEYIQHHLTNLTFGRHPDGTWGFAQSAQEAAAMGFN
                     AINVDSMGWSIGLGLVFVAVFYTAARKATSGVPGGLQNFVEIIIEFIDEITHSIFTHK
                     NDMIAPMALTVFVWVFLMNLMDLIPVDWIPGLAAAMGISHMKIVPTTDVNITMAMAGS
                     VFGLIIYYSIKCKGVGGFLGELAFHPLPKAAAPANLFLEGVTLIAKPLSLGLRLFGNM
                     YAGEMIFIL"
BASE COUNT          335 a          619 c          690 g          386 t
ORIGIN      
        1 cgaaggcgtt caggtcgccg aaatcgggct cgaccaggtg gtaccgaacc gctaccagcc
       61 ccgccgggat ttcaacgagg aagcccttgc cgagctggtc gcctccatca gatcccaggg
      121 agtgatgcag cccgttgtgg tcagacgacg agcccagggc ggatacgagc tcgtcgccgg
      181 tgagcggcgg tggcgtgctg ccggccttgc gggcctggac gccattccgg ccctgattcg
      241 tgatgttagc gatgagcagg cttcggccat ggcgctcatc gagaacatcc agcgcgagga
      301 tctcaacccg ctggaagagg cccacgccct gcagcggctg cgagacgagt tcggcctcac
      361 ccagcagcag gtcgccgatg cggtaggcaa gtcgcgcgtt gcggtgacca acctgctgcg
      421 cctgctgaat ctggggcctg cggtgcggga cctgctggtg gcaggagcga tcgagatggg
      481 ccatgcgcgc gccctgttgt cgctggacag cgttgttcag gagcgcgcgg ccaacgacgt
      541 ggtcgcccgg gggctttcgg ttcgccagac cgaggcgctg gtgaggcgcc tgcagcaggg
      601 caaggggccc gggcgcccgt ccgaggccgt ccgcgatgca gacaccgcgc acctggaacg
      661 cgaactctct gagaagatcg gcgcgccggt ttcgatctcc tgcactgcca agggccgagg
      721 tcgcctcgtc attcggtacg gatcgctcga ggagctcgac ggtatactcg gtcacatcag
      781 gtaggcgccg gcgccgctca gatgcgcgtc gttcgaaatg gccgctttgt caggatcacg
      841 ctaattgaac gcgctctggc ccgtctctat aatacgcgcc gcctttcggg ggttggtgtt
      901 tgtcaggctg tcctgacgtg gacatgtctg tgccgtatcg aaccgtggcg ctgcaggcgc
      961 tcgccgggct cggggtggtg ctggcagcgc tggcgtggga cgcccgtgaa gcggtggcgg
     1021 cgctggccgc tacgatcgtc agcgtcgtgc caaacgctta cttcgcctgg cgcgtcagcc
     1081 gcgagcgggc ggcagccagg atcctcgcgg cagaggtcgt gaaggtggtc gccaccgtgg
     1141 gcatgatggg attggccttt gcgacgatgg agttgcggcc tctgggtttc ttcgccacct
     1201 tcggcgtgct gcagctggcg cacgtggttg gcggaacgag cctggcggga aaagcgactt
     1261 cacgctgaac gccggcgcgt ttgttgacgc gctggtcaaa ttgccgggga cgtggtcttc
     1321 ggcagacatt ggggacgaga gcaacgcagc atgtcgagcg gaaccgtaac ctccggggag
     1381 tacatccagc atcacctgac gaacctgacg ttcggtcgcc atcctgacgg cacctggggg
     1441 tttgcccaaa gcgcccagga ggcggcggcc atggggttca atgccatcaa cgtggattcc
     1501 atgggatggt ccatcgggct cggcctggtt ttcgtggccg ttttctacac cgcggcgcgc
     1561 aaggctacgt ccggcgtccc gggcggtctg cagaacttcg tcgagatcat catcgagttc
     1621 atcgacgaga taacccacag catcttcacc cacaagaacg acatgatcgc gcccatggcg
     1681 ctcaccgtct tcgtctgggt gtttctcatg aacctgatgg acctgatccc ggtggattgg
     1741 atcccggggc tggccgcagc catggggatc agccacatga agatcgttcc gaccacggac
     1801 gtgaacatca ccatggccat ggccgggtcg gtgttcggcc tcatcatcta ctacagcatc
     1861 aaatgcaaag gtgtcggggg tttcctcggc gagctggcct tccacccctt gcccaaggcg
     1921 gccgcgccgg cgaacctgtt tctagagggg gtgaccctga ttgccaaacc gctttctctg
     1981 ggcctgcgac tgttcggcaa catgtatgcg ggggagatga tcttcatcct
//
LOCUS       DBNHQR010000380        11499 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1049373, whole genome shotgun sequence.
ACCESSION   DBNHQR010000380 DBNHQR010000000
VERSION     DBNHQR010000380.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 11499)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11499)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11499
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1049373"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="AC16VR_08605"
     CDS             complement(<1..905)
                     /locus_tag="AC16VR_08605"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6011255.1"
                     /translation="MRYATGMAMYFAQGIPKGLLHIAMPAWLASRGVAPGEIATYLAV
                     IVLPWAFKLVTGPLMDRYEFLPMGRRRPWVMFAQLGMVLSLLGLMIVEDPVEQMGTLI
                     LLGLVVNSFTATQDVAVDGMSIDFVPAREQGRLNAFMSFGKAIGWALTSAVSGVMLVT
                     WGLGVTAVAAAAGASVFFVALMFVREQPGERLLPWTAGQAASVHVEGKTFAAVFAGVN
                     RVLWQRGSLIVLAIMFFHGLWNGYGDALMPIAAINLFGYTTPQWSQLVAVMGLVGAGL
                     ALAAGPLIDRFGAKRMLVLTISLVAV"
     gene            complement(1031..3289)
                     /locus_tag="AC16VR_08610"
     CDS             complement(1031..3289)
                     /locus_tag="AC16VR_08610"
                     /inference="COORDINATES: protein motif:HMM:NF012907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HIR6011256.1"
                     /translation="MPKAVMIRCLIGWCAGLLAAAVAQAQTQNIVYFPEWGVHLQPYY
                     VRHIQDSGAADQLTVINYAFVVPAPNAAGDVVCQMDDPVAAYQQVYDAGMSVRVADTR
                     EADQPASVQRLFGHFNQFKKLKAERPDLKVLIAIGGWLGSVWWSDAAATPKSRETFVA
                     SCIDLFIRGNLPVAGTAGGEGVAAGVFDGFDIDWEYPITGGAGGTHHSANDDVNLTAT
                     LAEFRRQLDALPRAAGTDPYLLTMATPGSAFRGDNYQINADQAHVDWFNLMTYDFHGT
                     WENKTGHQTNILTSVNDPSSDAFKLSIDNAVRLYRDTYGVSVEKLVIGAAFYGRGWSG
                     VSTTDDGLYQSGRTAPGIYEDGYNYYRDLAPLMLGDYTLHWDPLALASWLYSPVERTF
                     WTLDEPQSIALKRRYADAFGLGGVMAWEVSGDDEDGTLVSAMRTGDPGAPVQSSSSAL
                     DGAVEVTQPASCAISVEGFNQVLGAETTVSAPAKVDQVEFFGNGVSLGFDNFPPWSWA
                     WFNLPAGVHEITAVASDWAGSSTSSTSVLLNVYSEADMALWQVGASYQPGDEVFYDGC
                     IYQAKRNHVGSRVRLPTGGRYWDLVTCSDCGGGGGGGGNQPPVIDSVTADPASGLQEG
                     DDVVITVVATDEDGSITKVAFSVEDEDWDGVASEPDTWTWTWSNVPAGTHEVTVTAHD
                     NSGATASDRITIVAASGSGCTAPAWDATLTYDKGDRVQHNDILWQAKRTVAEVEPGTS
                     PSKWTNLGPCTS"
     gene            complement(3326..4618)
                     /locus_tag="AC16VR_08615"
     CDS             complement(3326..4618)
                     /locus_tag="AC16VR_08615"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6011257.1"
                     /translation="MENPVCPASLAEATLADAEVLECPYPTYDLLREQAPVWQDPRTG
                     IFVVTRYEDLRMICTDTERFSNHRPSNDHDKLTGIARKAYELFVEKGWVPGESLAARD
                     DPEHRQMRSIFNEAFRPKRINALDSEVQALAYRLIDDFVDDGHCNFVKQFAVPLPLII
                     ICRQMGAREEDVWQIKAWTDAWFRGTGFNLTEEEVIWATEMEIQAQHYFQPIFERLRK
                     EPDGTLLSDLVNTEVPGWGRTLNDNELHAEMMQDTFVGGSETTTNALSAGIMLLAQNP
                     GVWERLKAEPEKYLRTFCEEVVRLESPVQGLTRMAKTDIELHGVTIPRGAVIDARFAA
                     GNRDPEHFDNPGEVDLDRPNAASHLGFSSGTHYCLGAPLARRELYWGFKAFLDNVASF
                     RLAPGRNDLRHYPNYALRALSELHIEFERVPSGDRTDI"
     gene            4682..5578
                     /locus_tag="AC16VR_08620"
     CDS             4682..5578
                     /locus_tag="AC16VR_08620"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6011258.1"
                     /translation="MTSGKRPDAGRYQDVFFESDDGLDLYARDYPNPDAPLTVLCLHG
                     LTRNSADFADLAAALQPDFRVLAMDQRGRGRSAYDPDPSRYALPTYVKDAFALMDHLA
                     LEHVAVIGTSMGGLMAMLMAARQPDRICGIVFNDVGPVVEAEGLRRIQGYVGKGGEIA
                     SWADAVALTQANNGAAFPGLSEADWLAFARRLFVENDAGVPVLAYDAKISEPMQADQT
                     TAVPADLWPVFDAMGEIPLLVVRGALSDILSRETVEEMARRHPGTTAVEVPDRGHAPM
                     LDEPEAMQAVSEFLAALGGKRG"
     gene            complement(5593..6876)
                     /locus_tag="AC16VR_08625"
     CDS             complement(5593..6876)
                     /locus_tag="AC16VR_08625"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6011259.1"
                     /translation="METEHQTGCPYRHADEIDFMDPVVQENWFDAYDIIREESPAYFM
                     PQIGMYVLTRYDDIEHVLRRPTQFTVGPDVQSTEPLIKHPEALALYEEKGWRRYTPLG
                     ENLPKHAHYRGLVDPALTAAAIRKRESFVRATVNELIDEWIDEGEIEFIDRFAEPLPM
                     IVIAELLGFPRMDLPQLKEWSYAWVLPFSRGLSLEQEMWAVNKHIELQHYIFETMQRK
                     RKDPKDDIITRLTRIEIEDVDIGAKRPLTDTEIIGITDHLLIGGNETTTFALSNGLWL
                     LFRNPDVYRALQADRDKIKNFVEEVLRIESPTQGLYRFVTEDCEIGGVPVPKGATLSI
                     RYGAGNHDPGRFPHPDTPELGRKNAGRHLAFGIGEHVCPGATLSRFEQRWAWEILLER
                     LRNIRPAPGKNDYKHRGGMWVRALEEIYMQFDKAG"
     gene            6987..7433
                     /locus_tag="AC16VR_08630"
     CDS             6987..7433
                     /locus_tag="AC16VR_08630"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6011260.1"
                     /translation="MIVGVHHVAIGVPDFETGLAFYRDVLGFEVLSASELSGNELADR
                     AIGLPDVRAQVAMLKAPNIYIELWSYSNPEPDDRRQRPCDFGYPHFALQVRDMQTEYA
                     RLSAAGMEFVGEPVELGGSAAIYGKDPFGNVIEIYEIRDPEIAQLS"
     gene            7522..7746
                     /locus_tag="AC16VR_08635"
     CDS             7522..7746
                     /locus_tag="AC16VR_08635"
                     /inference="COORDINATES: protein motif:HMM:NF016231.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HP1242/YdcH family protein"
                     /protein_id="HIR6011261.1"
                     /translation="MFEYEQDAVQALLKANDRFQDLYSQHAALKIQVHEAEIGVHPMD
                     DLTLNQLKRQKLLAKDKMAHMIQKYQRRHA"
     gene            complement(7786..8538)
                     /locus_tag="AC16VR_08640"
     CDS             complement(7786..8538)
                     /locus_tag="AC16VR_08640"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6011262.1"
                     /translation="MRLEGRTCVVTGGSSGIGAATVRRFVAEGAEVLIADIGVEQARG
                     LAAELGDAVSVVECDVRFEDSVRGVAAAAHARWDHVDVLVNNAGSELNRAYDEATVEE
                     WDRVQDTDLKGPWLMCKHLVPRMVERGSGSVINVSSLNGLVGFPLSTAYGGAKGGLVV
                     FTRDMAIELASSGVRFNCVCPGVIETPMMERWTDLMPDKGEAQEMLRGVMPIGRMGRA
                     DEVAGAILFFASDDSSLCQGAVLSVDGGFTAQ"
     gene            complement(8600..9469)
                     /locus_tag="AC16VR_08645"
     CDS             complement(8600..9469)
                     /locus_tag="AC16VR_08645"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6011263.1"
                     /translation="MKDFAGRTAVITGGASGIGLAMAQRFAEERMNVVLADIEENALR
                     AAVEHLEARQHRALGVVTDTMRRDAIEALLERAVAEFGNVHVLCNNAGVVVGGEHVPV
                     WEIPDADWEWVMGVNFYGVLYGLQTFVPHMLAHGEPGHIVNTASVAALMPGGDTYGVS
                     KHGVLVISEALHRDLRAAGSALGASVLCPGWVDTKIAEAERNRPTAMTSAKNPEGHGL
                     PLGDTLKQGMPPEVVAEKVLNSIREERFYILPHAGWDEVVLGRVDAIVARGAPYELDR
                     EALVAKRGLGEEV"
     gene            9570..10952
                     /locus_tag="AC16VR_08650"
     CDS             9570..10952
                     /locus_tag="AC16VR_08650"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010952140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HIR6011264.1"
                     /translation="MSGKTRTEKDSMGALEVPADALWSAQTQRAVENFPISGIRMPGA
                     FIRSLGLIKAAAAHVNADLGVLPAELALPIVEAAERVAAGSVDDHFPVDVFQTGSGTS
                     SNMNANEVIARLASDASGLEVHPNDHVNAGQSSNDVIPTAIHVSAVLATREDLLPALA
                     HLAGVLRERAAGLQGVVKTGRTHLMDAMPIRLGQEVGAWATQVEQDIARIRDTLPRCE
                     RLAQGGTAVGTGVNAHPEFAVRFARRLSDVTGVPFLPSEDLPASLASQDAAVELSGQL
                     KVTAVSLMKIANDLRWMNSGPLAGIAEIMLPALQPGSSIMPGKVNPVIPEAIAMVCAQ
                     VIGNDATVTVAGQSGNFQLNVMLPVIAYNLIQSIELLGSGARLLADKALKEFEVAEDT
                     ISERLARNPILVTALNARVGYDAGAAIAKRAYAEGRSVLEVALEMTTLSREELERLLD
                     PAALAGPPRS"
     gene            complement(10977..11381)
                     /locus_tag="AC16VR_08655"
     CDS             complement(10977..11381)
                     /locus_tag="AC16VR_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005344 - oxygen carrier activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0015671 - oxygen transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin"
                     /protein_id="HIR6011265.1"
                     /translation="MSDLVLESLDAAAETGQDVTEAVNRAFRDREPQAAGLMDHMDEH
                     MLGRMMQEVLMVLMSENPEDQLEYLNFEVGSHLGYGVTAPMYGALFAAVRDCVRDLVG
                     EAWSPATETAWKQRIRSLQEIVETTAKAPADA"
BASE COUNT         2027 a         3864 c         3691 g         1917 t
ORIGIN      
        1 acggccacca gcgagatggt cagcaccagc atgcgcttgg cgccgaagcg gtcgatgagg
       61 ggcccggccg ccagcgccaa acccgcaccc accaggccca tgacggccac gagctgggac
      121 cactggggcg tggtgtagcc aaagaggttg atggccgcga tgggcatgag cgcatcgccg
      181 tagccgttcc agagcccgtg aaagaacatg atggccagaa cgatgaggct cccccgctgc
      241 catagcaccc ggttcacacc agcaaagacg gcagcgaacg tcttgccctc cacgtgcacg
      301 gaggccgcct gaccggccgt ccagggaagc agacgctcgc cgggctgctc ccggacgaac
      361 atcagggcga cgaagaagac cgacgcgccg gccgcggccg cgaccgcggt caccccgagg
      421 ccccaggtca ccagcattac cccggaaacg gccgaggtca gcgcccagcc gatggctttg
      481 ccgaagctca tgaacgcgtt cagtcgcccc tgttcccggg cgggaacgaa gtcgatggac
      541 atgccgtcca cggccacgtc ctgggtcgcc gtgaacgagt tgacgaccag gccgagcagg
      601 atgagcgtgc ccatctgctc aacgggatcc tcgacgatca tgagcccgag caggctcagc
      661 accatgccga gctgcgcaaa catcacccag ggccggcggc ggcccatggg cagaaactcg
      721 tagcgatcca tgagagggcc ggtgaccagc ttgaacgccc agggcagcac gatcacggca
      781 aggtaggtgg cgatctcgcc gggggcgacg ccacggctgg cgagccaggc cggcatggcg
      841 atgtgcagca gccccttggg aatgccctgg gcaaaataca tggccatgcc ggtggcgtaa
      901 cgcacaccgg cgctgtcaga cagctgcaga gcagtttgca cgatgctttt gagccctcac
      961 cccgaagacg cgatgatacc ccgtccgccg ctattttaga gcgctttgag cctatctcct
     1021 gcgcgcccga tcaggacgtg cagggcccca gattcgtcca cttggacggg ctggtgcccg
     1081 gctcgacctc ggcaacggtg cgcttggctt gccagaggat gtcgttgtgc tgaacccggt
     1141 cgcccttgtc gtaagtcagc gttgcgtccc atgccggcgc cgtgcagccg gagccgctcg
     1201 ccgcgacgat ggtgattctg tcgctggcgg tggcgcctga gttgtcgtgg gcggtgacgg
     1261 tgacctcgtg agtgccggcg gggacgttgg accaggtcca ggtccaggta tccggttccg
     1321 acgcgacccc atcccaatcc tcatcctcga cggagaacgc gactttcgta atcgagccgt
     1381 cctcgtccgt tgcaacaacg gtaatgacca cgtcatcccc ttcctgcaat ccgctggcag
     1441 gatcagccgt gacgctatcg atcacgggag gctgattgcc gcctccacct ccgccaccgc
     1501 cgcagtcgct gcaggtcacc agatcccagt agcgcccgcc ggtcggcagg cggacccggg
     1561 agcccacgtg gttgcgcttg gcctggtaga tgcagccgtc gtagaacacc tcgtcgcccg
     1621 gctggtagct ggcgcccacc tgccacagcg ccatgtctgc ttcggagtag acgttcagta
     1681 ataccgaggt cgaactggtc gagctacccg cccagtccga ggcgaccgcc gtaatctcgt
     1741 gaaccccggc gggcaggttg aaccaggccc agctccaggg cggaaaattg tcaaaaccca
     1801 gggaaacccc gttgccgaag aactccacct gatccacctt ggcgggcgcc gacacggtcg
     1861 tctcagcgcc gaggacctga ttgaacccct ccacggagat ggcgcaactc gccggctgcg
     1921 tcacttcgac ggccccgtcc aaggcagaac tcgaagattg gactggagcc ccggggtcac
     1981 cggttcgcat cgcgcttacc agggtcccat cttcgtcatc gccgctcact tcccaggcca
     2041 tcaccccacc aagcccgaag gcatccgcgt agcgccgctt gagcgcaatc gactgaggct
     2101 cgtccagggt ccagaaggtc cgctccacgg ggctgtaaag ccaggaggcc agcgccagcg
     2161 gatcccagtg cagggtgtag tcaccgagca tcagtggtgc caggtcgcgg tagtagttgt
     2221 agccgtcctc gtagatgccg ggcgccgtcc gtccgctctg gtaaaggccg tcgtccgtcg
     2281 tgcttacccc gctccagccg cggccgtaga aggcagcgcc gatcacgagc ttctccacgg
     2341 aaacgccgta ggtgtcgcgg taaaggcgca cggcgttgtc aatcgacagc ttgaacgcat
     2401 ccgaggaggg gtcgttcacg gaggtcagga tattggtctg gtgacccgtc ttgttctccc
     2461 aggtgccgtg aaagtcgtag gtcatcaggt tgaaccagtc cacgtgcgcc tgatcggcgt
     2521 tgatctggta gttgtcgccg cggaacgcgc tccccggggt ggccatggtg agcaggtatg
     2581 ggtccgtgcc agcagcgcga ggcaaagcat cgagctgccg gcggaactcg gcaagggtcg
     2641 cggtgaggtt cacgtcgtcg ttggcgctgt ggtgggtccc gcccgccccg ccggtgatgg
     2701 ggtactccca gtcgatgtcg aagccgtcga agacgccggc cgccacgcct tcgccgcccg
     2761 cggtgcccgc caccggcagg ttgccgcgga tgaagaggtc gatgcacgac gccacgaacg
     2821 tctcgcggga cttgggcgtt gccgccgcat ccgaccacca caccgagccg agccacccgc
     2881 cgatggcgat cagcacctta agatccggac gctcggcctt gagctttttg aactggttga
     2941 agtgaccgaa cagcctctga actgaggcgg gctgatcggc ttcccgtgta tcggcaacgc
     3001 gcacgctcat gcctgcgtcg tacacctgct gataagcggc caccgggtcg tccatctggc
     3061 agacgacgtc gccggccgcg ttgggtgccg gcacgacgaa ggcgtagttg atgacggtga
     3121 gctgatcggc ggcacccgaa tcctgaatat gcctcacgta gtagggctgc aggtggacgc
     3181 cccactcggg aaagtagacg atgttctgcg tctgggcctg cgcaaccgca gcagccagca
     3241 agcccgcaca ccagccaatc aggcaccgaa tcatgacggc cttgggcata gctcccacct
     3301 cgaactataa agacaatatg cctgactata tatcagttcg gtcgccggac ggaacccgct
     3361 cgaactcgat atgcagctcg ctcaaggccc gcaacgcgta gttggggtag tggcgcaggt
     3421 cgttcctgcc gggggcgagc cggaaggagg cgacgttgtc gagaaaggcc ttgaagcccc
     3481 agtaaagctc ccggcgcgcc agcggggccc ccaggcagta gtgggtgccg gagctgaagc
     3541 ccagatgaga agccgcgtta gggcggtcca ggtcgacttc gcccgggtta tcgaagtgct
     3601 ccgggtcccg attgccggcg gcaaagcggg cgtcgatcac ggcaccgcgg gggatcgtga
     3661 cgccgtggag ctcgatgtcc gtcttggcca tgcgggtgag cccctgaacg gggctctcca
     3721 gccggaccac ctcctcgcag aacgtgcgca ggtacttctc cggctccgcc ttcaaccgct
     3781 cccacacgcc cggattctgg gccagcagca tgatgccggc ggaaagcgcg ttggtggtgg
     3841 tctccgagcc gcccacgaac gtatcctgca tcatctcggc gtgcagctcg ttgtcgttga
     3901 gcgtcctgcc ccagccgggc acctcggtgt tcaccagatc cgacagcagg gtgccgtccg
     3961 gctctttgcg cagccgctcg aaaatgggct ggaagtagtg ctgggcctgg atctccatct
     4021 ccgtggccca gatcacctcc tcttcggtga ggttgaagcc ggtgcccctg aaccaggcgt
     4081 ccgtccacgc cttgatctgc cagacgtcct cctccctcgc gcccatctgc cggcagatga
     4141 tgatcagcgg caggggcacg gcgaactgct tgacgaagtt gcagtggccg tcgtcgacga
     4201 agtcgtcgat cagacggtag gcgagcgcct gcacctccga atccagcgcg ttgatccgct
     4261 tggggcggaa ggcttcgtta aagatggagc gcatctgccg gtgctccggg tcgtcccgag
     4321 ccgccagcga ttcgccgggc acccagccct tctctacaaa gagctcgtag gccttgcgcg
     4381 cgatgccggt gagcttgtcg tgatcgttgc tcggccggtg gttgctgaag cgctcggtgt
     4441 ccgtgcagat catacgcaga tcctcgtagc gggtgaccac gaagatgccc gtgcgcgggt
     4501 cctgccagac gggggcctgc tcgcgcagaa ggtcgtaggt gggatagggg cactcgagga
     4561 cctcggcgtc cgccagggtg gcctcggcca ggctcgccgg gcagaccgga ttctccattc
     4621 ggttgatttc tgtcattccg ttaccatgcg catctttcgc cacgctaatg ctgccatgac
     4681 catgacttca ggcaagcgcc ccgacgcggg ccgctaccag gacgtgtttt tcgaaagcga
     4741 cgacgggctg gatctttacg cccgcgacta ccccaacccg gacgcgccgc tgaccgtgct
     4801 ctgcctgcac gggctgaccc gcaactcggc agattttgcg gatctggccg cggcgctcca
     4861 gccggacttt cgcgtgctgg ccatggacca gcgaggccgg ggccggtccg cttacgatcc
     4921 cgacccgagc cgttacgcgc tgccgacata cgtgaaggac gcgtttgccc tgatggacca
     4981 cctggcgctc gagcacgtgg ccgtcatcgg cacctccatg ggcgggctca tggccatgct
     5041 catggccgcg cggcagccgg accgtatctg cggcatcgtt ttcaacgacg ttggcccggt
     5101 ggtggaggcc gaaggtctgc gccgcatcca gggctacgtg ggtaagggcg gcgagatcgc
     5161 ctcgtgggca gacgcggtgg cgctgaccca ggccaacaac ggcgcggctt tccctggctt
     5221 gagcgaggcc gactggctcg ccttcgcccg gcgcctgttc gtcgagaacg acgctggcgt
     5281 tccggtgctg gcctacgatg cgaagatctc cgagccaatg caggcggacc agaccacggc
     5341 cgtacccgcc gatctctggc ccgtcttcga cgccatgggc gagattccgc tgctggtggt
     5401 gcgcggcgcg ctctcggaca ttctctcccg ggaaaccgtc gaggagatgg cgcggcgcca
     5461 tcccggcacc accgctgtgg aggtgcccga tcgcggccac gcgccgatgc tggacgaacc
     5521 ggaagccatg caggcggtga gcgagtttct cgccgcgctc ggcggcaaac gcggctgagg
     5581 tcctgacagg cgctatcccg ccttgtcgaa ctgcatgtag atctcctcga gggcgcggac
     5641 ccacatgccg ccgcggtgct tgtagtcgtt cttgcccggc gccggccgga tgttgcgcag
     5701 ccgctcgagc aggatctccc aggcccagcg ctgctcgaag cggctcaggg tggcacctgg
     5761 gcagacgtgc tcgccgatcc cgaacgccag gtgccggccc gcgttcttgc gacccagctc
     5821 cggcgtgtcc gggtgcggaa agcgcccggg gtcgtgattc ccggcaccgt agcggatgct
     5881 gagggtagcg cccttgggaa cgggaacccc gccgatctcg cagtcctcgg tgacgaaccg
     5941 gtagaggccc tgagtggggg attcgatgcg cagcacctct tcgacgaagt tcttgatctt
     6001 gtcgcgatcg gcctgcaatg cccggtagac gtccgggttg cgaaagagca gccacagccc
     6061 gttggaaagc gcgaacgtgg tggtctcgtt gccgccgatc agcaggtgat cggtgatgcc
     6121 gatgatctcc gtgtcggtga gcgggcgctt ggcgccgatg tccacgtcct cgatctcgat
     6181 ccgggtgagc cgggtgatga tgtcgtcctt cggatccttc cgtttgcgct gcatggtctc
     6241 gaagatgtag tgctgcagct cgatgtgctt gttcacagcc cacatttcct gctcgaggga
     6301 cagcccgcgg gaaaacggca gcacccaggc gtaggaccac tccttgagct ggggcagatc
     6361 catgcgggga aaaccgagga gctcggcgat gacgatcatc ggcagcggct cggcgaaccg
     6421 gtcgatgaac tcgatctcgc cctcgtcgat ccactcgtcg atgagctcgt tgacggtagc
     6481 gcgcacgaag ctttcacgtt tcctgatggc cgccgccgtc agcgcagggt ccaccaaccc
     6541 acggtagtgg gcgtgcttgg gcaggttctc accaagcggc gtgtagcgac gccagccttt
     6601 ttcctcgtat agcgcgagcg cctcgggatg cttgatgagc ggttcggtgc tctgcacgtc
     6661 cgggcccacc gtgaactggg tcggccggcg cagcacgtgc tcgatgtcgt cgtagcgggt
     6721 cagcacgtac atgccgatct ggggcatgaa gtaggcgggc gactcctcac gaatgatgtc
     6781 gtaggcgtcg aaccagttct cctgcaccac cggatccatg aaatcgatct cgtccgcgtg
     6841 ccggtacgga caccccgtct gatgctcggt ctccatgatg ctcccctctc ccttaaaacc
     6901 ctgccaacta tacaatttat acaattgcgc cccgaagaga cggtaccatc aaggccctca
     6961 gcattcagca acgcaagggg atagcaatga tcgtaggcgt tcaccacgtg gccatcggcg
     7021 ttccggattt cgagacgggc ctggcctttt atcgggacgt gctcggtttc gaggtcttga
     7081 gcgcaagcga gctctccgga aatgagctcg ccgaccgggc catcgggctg cccgacgtgc
     7141 gggcccaggt cgccatgctg aaagcaccaa acatctacat cgagctgtgg agctacagca
     7201 acccggagcc ggacgacaga cgccagcgcc cgtgcgactt cggctacccc cacttcgcgc
     7261 tgcaggtcag ggacatgcag accgagtacg caaggctctc ggcagccggc atggaattcg
     7321 tcggcgagcc ggtggagctc ggcgggtccg ccgccatcta cggtaaggac ccgttcggaa
     7381 acgtcatcga gatctacgag atccgcgatc ccgaaatcgc ccagctgagc tgacgacctc
     7441 cgccgaaaca gccccgcagt cttgtaagcc acggaatttt tgactagttt cagacgtgca
     7501 caacctggga aggggtgaag catgttcgag tacgaacagg atgcggtgca ggcgcttctg
     7561 aaagcgaacg accgctttca ggacctctac agccagcacg ctgcgctgaa gatccaggtc
     7621 cacgaggcgg agatcggcgt ccatcctatg gatgatctga ccctaaacca actcaagcgc
     7681 cagaagctcc tcgccaagga caagatggcg cacatgatcc agaaatacca acgccggcac
     7741 gcctgacccc gacccgcggg gcctgccgac gtccatctgc gcggcctact gggcggtaaa
     7801 gcctccgtcc acggagagta cggcgccctg gcagaggctg gagtcgtcgg aggcgaagaa
     7861 cagaattgct ccagcaacct catccgcccg gcccatgcgg ccgatgggca tgacgccccg
     7921 caacatttcc tgagcctctc ccttgtcggg catcagatct gtccagcgtt ccatcatggg
     7981 ggtctcgatg acgcccgggc agacacagtt gaaacgcacc ccggagctgg cgagctcgat
     8041 ggccatgtcg cgggtgaaga ccaccagacc gcccttggcc ccaccgtagg cggtcgacag
     8101 aggaaacccc accaggccgt tgagcgagga tacgttgatc acgctgccgc tgccgcgctc
     8161 caccatccgg gggaccaggt gcttgcacat gagccaggga cccttcagat cggtgtcctg
     8221 aacccggtcc cattcctcaa cggtggcctc gtcgtaggcg cggttgagct ccgagcccgc
     8281 gttgttgaca agcacgtcca catggtccca ccgcgcatgg gccgcggccg ccacgccgcg
     8341 cacgctgtcc tcgaagcgca cgtcgcactc caccacgctc accgcatcgc cgagctccgc
     8401 ggcaagcccg cgcgcctgct cgacgccgat gtcggcaatc agcacctcgg ccccctcagc
     8461 cacgaaccgg cgaacggtgg cggccccgat gccgctcgac ccgcccgtca ccacgcacgt
     8521 tctgccctca agcctcatcc tgtctctcct tcaacctggt cccccggccc gcggctcgcg
     8581 cctgccaggc cggcaatgct catacttcct cgccgagacc ccgcttggcc accagggctt
     8641 cgcgatcgag ctcgtaaggc gcaccccggg cgacgatggc gtcgaccctg cccagaacca
     8701 cctcgtccca gccggcgtgg gggaggatgt agaaccgctc ctcccggatg gagttcagca
     8761 ccttttccgc caccacctcc ggcggcatac cctgcttgag ggtatctccc agcggcagcc
     8821 catgcccttc ggggtttttc gcgctagtca tggccgtggg ccggttgcgc tcggcttcgg
     8881 cgatcttcgt atccacccac cccgggcaga ggacggacgc gccgagggcc gaaccggccg
     8941 cgcggagatc ccggtgaagc gcctcgctga tgaccagcac cccgtgcttg ctgacgccgt
     9001 aggtgtcgcc cccgggcatg agggcggcga ccgacgccgt gttgacgatg tgcccgggct
     9061 cgccgtgggc cagcatgtgc ggcacgaagg tctggagccc gtaaagaacc ccgtagaagt
     9121 tgacgcccat gacccattcc cagtcggcgt ccggaatctc ccagacgggc acgtgctcgc
     9181 cgccgacgac gacgcccgcg ttgttgcaga gcacgtgaac gttcccgaac tcggcaacgg
     9241 cgcgctcgag aagcgcttcg atagcgtcgc gccgcatggt gtccgtgacc acgccgaggg
     9301 cccggtgctg acgggcttcg agatgctcga ccgcagcgcg cagcgcattc tcttcgatat
     9361 ccgcgagcac cacgttcatg cgctcctcgg caaaccgctg ggccatggca agcccgatgc
     9421 cgctggcacc cccggtgatg accgccgtgc ggcccgcaaa gtctttcatg tccgctcccc
     9481 gaacgagcgt tctaagataa caggattgcg cggcgcctct acaccgatcg gttaagatcc
     9541 tgctgctgcc gcgcgatcag gagaaaccca tgagcggaaa gacgcgaacc gagaaagaca
     9601 gcatgggagc gctcgaggtg cccgccgatg ccttgtggtc ggcacagacc cagcgcgccg
     9661 tggagaactt ccccatcagc gggatcagga tgcccggcgc cttcatccgt tcgctgggcc
     9721 tgatcaaggc cgccgcggca cacgtcaacg cggatctcgg cgtgcttccg gcggagcttg
     9781 cgctgcccat cgtcgaggcc gccgagcggg tcgccgccgg cagcgtggac gatcacttcc
     9841 ccgtggacgt cttccagacc ggatcgggca ccagcagcaa catgaacgcc aacgaggtca
     9901 tcgcccgcct tgcatccgat gcatcggggc tggaggtgca tcccaacgat cacgtgaatg
     9961 ccggccagag cagcaacgac gtgatcccca cggccatcca cgtcagcgca gtgctcgcca
    10021 cgcgggaaga cctgttgccg gcattggcgc atctggccgg cgtcctgaga gagcgggcgg
    10081 ccgggttgca gggcgtggtg aagacgggcc gcacgcacct catggacgcc atgcccatcc
    10141 ggttgggcca ggaggtgggg gcgtgggcca ctcaggtgga gcaggacatc gcccgcattc
    10201 gcgacaccct gccacgctgt gagcgcctcg cccagggcgg caccgcggtg ggaaccggcg
    10261 tcaacgcgca tccggagttc gccgtgcggt tcgcccgtcg cctcagcgac gtcaccggcg
    10321 ttcccttcct ccccagcgaa gatctgcccg caagcctcgc cagccaggac gctgccgttg
    10381 agctctccgg gcagctgaag gtcaccgcgg tgagcctcat gaagatcgcc aacgacctgc
    10441 gctggatgaa cagcgggccg ctggccggca tcgcggagat catgctgccg gcgcttcaac
    10501 ccggcagcag catcatgccg ggcaaggtca atccggtaat ccccgaagcc atcgccatgg
    10561 tctgcgccca ggtgatcggc aacgacgcca cggtgacggt ggccggccag tcggggaact
    10621 tccagctgaa cgtaatgctg cccgtcatcg cgtacaacct gattcagagc atcgagctgc
    10681 tcggcagtgg cgcccggctg ctggccgaca aggcgctcaa ggaattcgag gtggcggagg
    10741 acacgatcag cgaacggctc gcgcgcaacc ccattctggt gaccgcgctg aacgcgcggg
    10801 tgggctacga tgccggggcg gccatcgcca aacgggcgta cgccgagggc cgctcggtgc
    10861 tcgaggtcgc tctggagatg acgacgctca gccgcgagga gctcgagcgc ctgctggacc
    10921 ccgctgcgct cgccggaccg ccgcgaagct gaaccgcccg tccggcggcg cctcgctcac
    10981 gcgtcggcgg gagctttcgc ggtggtctcg acgatctcct gaaggcttcg gatgcgctgt
    11041 ttccaggccg tttccgtggc cgggctccag gcctcgccga ccaggtctcg cacgcagtcg
    11101 cgcaccgcgg caaacagggc gccgtacatg ggcgcggtaa cgccgtaacc aaggtgcgag
    11161 ccgacctcga agttcagata ctccagctga tcctcggggt tctcggacat cagcaccatg
    11221 agcacctcct gcatcatgcg accaagcatg tgctcgtcca tgtgatccat gaggcccgcc
    11281 gcttggggtt cccggtcgcg gaaggcccgg ttgacggcct cggtcacgtc ctgaccggtt
    11341 tcggccgcgg cgtccaagga ttccagaacc agatcagaca tcgccagtct ccgacgaagg
    11401 ttggccggga ggcgccgaag gatgcctgag cacgaccatc accaccagcg cgccaaggag
    11461 catcagcagt cccgccagaa gaaacgaaac gccggggaa
//
LOCUS       DBNHQR010000381         2168 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_796800, whole genome shotgun sequence.
ACCESSION   DBNHQR010000381 DBNHQR010000000
VERSION     DBNHQR010000381.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_796800"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..1378)
                     /gene="selA"
                     /locus_tag="AC16VR_08660"
     CDS             complement(11..1378)
                     /gene="selA"
                     /locus_tag="AC16VR_08660"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HIR6011266.1"
                     /translation="MPRLRDLPSIDRLLRSPKLAFLVHAHGAETIKQSLRSIQAAWRA
                     TGEVPEWATDPEEYARRLEDQLAGASYRPVFNLTGTMIHTNLGRALLDPEMFRSIEPL
                     VTRPVNLEFDLASGRRGDRDRVVESRLQVLTGAPAATVVNNNAAALMLCLNTAALGRE
                     VIVSRGELIEIGGSFRLPELMERAGCRLVEVGTTNRTHLRDYQAAIGERTGMLLKVHP
                     SNYHISGFTHDVGVAELAALAREHRLPLCVDLGSGTLVDLRKWGLPHEPMPQEILAQG
                     ADLVTFSGDKLLGSVQAGIIVGDSENVGAIKRNPMKRALRPDKLTLAFLDRTLRLYEN
                     PERLAERLPLLRSLTRTLGELENQGEVLARSLETVLGDFDVRLVPSEVQIGSGSLPNQ
                     RIASRAVRITHDDPSEVRRLGERLRRLATPVIGRLHQEAVWLDVRGAEPLDELIEVLH
                     GLAAP"
     gene            complement(1432..>2168)
                     /locus_tag="AC16VR_08665"
     CDS             complement(1432..>2168)
                     /locus_tag="AC16VR_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017148197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HIR6011267.1"
                     /translation="FVYPRNDLAYAENFLHMMFATPCEDYEVNPIIARAMDRIFLLHA
                     DHEQNASTSTVRLAGSTGANPFACIAAGIAALWGPAHGGANEAVLNMLDEIGDEKHID
                     KYVKRAKDKDDPFRIMGFGHRVYKNYDPRAKVMQQTCHEVLDELGVENDPVLRMAQRL
                     EKIALEDEYFVERKLYPNVDFYSGIILKAIGIPTEMFTVIFATGRTPGWIAHWNEMIG
                     APYKIGRPRQLYQGATPRDYVPLAER"
BASE COUNT          360 a          677 c          732 g          399 t
ORIGIN      
        1 agggtaacga tcacggcgcg gcgagcccgt gcagcacctc gatgagctcg tcgagcggct
       61 cggcgcctcg aacgtccagc cagacggcct cctgatgaag cctgccgatc accggcgtgg
      121 ctagcctacg cagacgttcg ccgagcctgc ggacctcgga ggggtcgtca tgggtgatgc
      181 gcactgcccg gctcgcgatg cgttggttgg gcaggcttcc gctaccgatc tgcacttcgc
      241 tgggcaccag gcgcacgtcg aagtcgccca gaacggtctc cagtgatcga gccagcactt
      301 cgccctggtt ttccagctcg cccagggtgc gggtgagcga gcgcaggagg ggaagccttt
      361 cggccagacg ctcggggttt tcgtagagcc tgagagtccg gtccagaaag gccagggtca
      421 acttgtccgg acgcagagcg cgcttcatgg ggtttcgctt tatcgcgccg acgttttccg
      481 agtcgcccac gatgatgccc gcctgaacgg agcccagcag cttgtctccg ctgaacgtca
      541 ccagatctgc gccctgggcg agaatctctt gaggcatggg ttcgtgaggc agcccccact
      601 tccggaggtc gacgagcgtg ccgctgccga ggtccacaca caggggcagc cggtgctccc
      661 gtgccagagc cgccagctcc gcgacgccga catcgtgggt gaaaccgctg atgtgatagt
      721 tggatggatg cactttcagc agcattcccg ttcgctcgcc gatcgcagcc tggtagtcgc
      781 gaaggtgggt gcggttggtg gttcccacct ccaccaggcg acacccggcg cgctccatca
      841 gctcgggaag cctgaagctg ccgccgatct cgatgagctc gccacgggaa acgatcacct
      901 cccgcccgag cgcggccgtg ttcaggcaca acatcagggc ggccgcgttg ttgttgacca
      961 cggtggcggc cggagcacca gtgagcacct gcagccggga ctcgaccacc cgatcccggt
     1021 cgcctcgccg gccgctggca agatcgaact ccaggttcac gggccgggtc accagaggct
     1081 cgatggaccg gaacatctcc ggatcgagca gagcgcggcc taggttggtg tggatcattg
     1141 tcccggtgag gttgaatacc gggcgatagg aggcgccggc gagctgatct tccagccgcc
     1201 gcgcgtactc ctcgggatcc gttgcccact ccgggacctc gccggtggcc cgccaggccg
     1261 cctgtattga tctcaatgac tgcttgatgg tttccgcgcc gtgggcgtgg accaggaagg
     1321 cgagcttcgg ggagcgaagc agccggtcga tgctgggcag gtcacgcagt cgtggcatcg
     1381 tcgatctctc ggcggagagt ccccggcgcg gaaaacgggc cgggaccttt gtcagcgctc
     1441 ggcgaggggg acgtagtccc ggggcgtcgc gccctggtag agctgtcgcg gtcgtccgat
     1501 cttgtaaggc gcgccgatca tctcgttcca atgggcaatc cagccagggg tgcggccggt
     1561 ggcgaagata acggtgaaca tctcggtggg aatgccgatg gccttgagga tgatccccga
     1621 gtagaagtcg acgttggggt acagcttgcg ctcgacgaag tactcgtcct ccagcgcgat
     1681 cttctccagg cgctgagcca tgcgcagcac cggatcgttc tccacgccga gctcgtcgag
     1741 cacctcatga cacgtctgct gcatgacctt ggcacgcgga tcgtagttct tgtacacccg
     1801 gtggccgaaa cccatgatcc ggaacgggtc gtccttgtcc ttggcgcgtt tgacgtactt
     1861 gtcgatgtgc ttctcgtcgc cgatctcatc gagcatgttg agcacggctt cgttggcccc
     1921 tccgtgcgcg gggccccaca gtgcggcaat gccggcagcg atgcacgcga aggggttggc
     1981 gcccgtggag ccggccagcc tgacggtgga cgtcgaggca ttctgctcat ggtctgcgtg
     2041 caggaggaaa atacgatcca tcgcgcgggc gatgatcggg ttgacctcgt aatcctcgca
     2101 cggcgtggca aacatcatgt gcaggaaatt ctcggcgtag gcgaggtcgt tcctggggta
     2161 gacgaagg
//
LOCUS       DBNHQR010000382        10624 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1682384, whole genome shotgun sequence.
ACCESSION   DBNHQR010000382 DBNHQR010000000
VERSION     DBNHQR010000382.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10624)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10624)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10624
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1682384"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="AC16VR_08670"
     CDS             complement(<1..884)
                     /locus_tag="AC16VR_08670"
                     /inference="COORDINATES: protein motif:HMM:NF017773.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6011268.1"
                     /translation="MITERANPSLRLFAAVAMLAALTACAAKGPYVLELMPAPTVYQD
                     EDFSPFADDFSPESLPYRGMLYVTDRRPAGAGKPPDPADPHYVGKRGEGLRAGVASIQ
                     LGEGGFTWEEAIRVSMASDRGEGYRLSVTGVEELGFLEPSIEPFIEAPDFPLADEAFA
                     ALVNDKLAQSEQKDVYIYVHGYRVRFEYPVLVATELWHFLGYEGAFIAFSWPSTPSYL
                     AYFADAEVAVVSARQLRILLEFLAQSTDVRRIHILGYSQGTRTVMQALHMLALKYEED
                     DRESLLERLRIGNVALVG"
     gene            1094..1807
                     /locus_tag="AC16VR_08675"
     CDS             1094..1807
                     /locus_tag="AC16VR_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011269.1"
                     /translation="MAALLMLGSSGLRAEQGDETWWSITPYIWAMDTKLDLSWRDSPV
                     GGTEIDFKDALGNLDAAFQVAVEGGRGRWSAFADVTFVKISDATGEDALRLKTKNKQV
                     FLDAGVAFWPGGAGSPLSIYGGARYTGFDNTYRLQFQGDDVARRDSSADYFDALLGVR
                     YRFDLSDRWSILTLGDVSFGESEGSWQAQALAAYTVGKRRMNRIMFGYRYRDTEFDDA
                     ELTTEYGFHGPLVGFNFRF"
     gene            1929..2729
                     /locus_tag="AC16VR_08680"
     CDS             1929..2729
                     /locus_tag="AC16VR_08680"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6011270.1"
                     /translation="MSDSEIVLCEIDDGTATLILNRPEVLNAMNDELMGGICRAMERV
                     VEDESVRVVILTGAGRGFCAGADLARAAEGPADPDLQDAPDSGDLFNGAVSALMSCPV
                     PTIARVNGPAAGGGLGLALACDVAIAAESAIFVATFGPNLGIVPDLGSTWSIPMRVGR
                     ARALGMALLGDRITANQAEAWGLIWRSVPDEELDAEVGRVAMVLKRSSPSACTRIREC
                     VDAAIHHTFGDQLGLEMAHQGVLIPRNMQEGARAFMEKRPPEFGGDRG"
     gene            2774..3739
                     /locus_tag="AC16VR_08685"
     CDS             2774..3739
                     /locus_tag="AC16VR_08685"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR6011271.1"
                     /translation="MRDSEILQRLEKRLGTAHARERLGVETAHESRLFSHGIRFFHPE
                     NWYSVHSIIRNSLKVSGLYARASRNALDHRVVENHVVLDALPAGLDGLRVLHLSDLHV
                     DVSDELLHAIIERVRELTYDVCVLTGDYRFRTYGAIDDALRGMARLRSNLDGSVYAVF
                     GNHDSVTMLPALEDMGIRMLMNESHAHRHGDDHLYLAGIDDAHFFGVHDIEKALAGVP
                     DTVPRILLSHTPEVYRQAARAGVQLLLCGHTHGGQICLPGGYPVTLDSRVPRRLGRGC
                     WDYKGMRGYTSPGAGASMVAVRLNCPPELTVHVFHSAGPGAAQLQ"
     gene            complement(3733..4227)
                     /locus_tag="AC16VR_08690"
     CDS             complement(3733..4227)
                     /locus_tag="AC16VR_08690"
                     /inference="COORDINATES: protein motif:HMM:NF013981.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HIR6011272.1"
                     /translation="MNLDLILALKVLALALSANGAPVIGKRLFGARWAIPLDGGLTFL
                     DGRPLLGSSKTVRGLVLSLATTTLVATALGYGWQLGVIYSALTMSGDALSSFVKRRRG
                     IPPSGRFRGLDQIPETALPMWVCSSALGLTGWDIAVLVALFFAGDFVLSKVLYRIGIR
                     DEPY"
     gene            complement(4251..5351)
                     /locus_tag="AC16VR_08695"
     CDS             complement(4251..5351)
                     /locus_tag="AC16VR_08695"
                     /inference="COORDINATES: protein motif:HMM:NF013394.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylethanolamine N-methyltransferase
                     family domain-containing protein"
                     /protein_id="HIR6011273.1"
                     /translation="MSGSLLAGWFAPLAVYLAILILHLALPARRVDGYALDPGTGQPL
                     TYRLNGLVVFALVTSVWLAAGWKGWISWDWLYVNRWSAMGGACLVGLAYSLITVLGAP
                     PTGRSLLADLYLGRPENPQHFNKRVDAKMFLYLAGAVLLGLNTLSATAFHAGALGSAS
                     NPGVYLHAALLTFFVLDYLVFERVHLYTYDLFAERVGLKLGWGCLAFYPFFYPVGLWG
                     TAHLGKPSVIADLGWIWLALSALVFLSGWVLARGANLQKYVFKRFPERAFLGRFPPRV
                     IHGGNLALLCGGFWGVARHVNYLGEILMATGLAMSLGHLDSPWPWLYPLYYLALLVPR
                     ERDDDRRCAEKYGTLWDEYRRRVPYRIVPRIY"
     gene            5484..7130
                     /gene="betA"
                     /locus_tag="AC16VR_08700"
     CDS             5484..7130
                     /gene="betA"
                     /locus_tag="AC16VR_08700"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF002550.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HIR6011274.1"
                     /translation="MEVFDYIIAGAGSAGCVLANRLSEDGARSVLLLEAGGSDRHLFV
                     RMPSAFSLPIGRPRFDWCYQAEAEPALDGRSLACPRGRVLGGSSSINGMVYVRGHPRD
                     FERWESLGATGWGYADVLPYFKRAEHVLGAAEGDPYRGRAGPLTVSRGRRANPLYETF
                     LQAAEQAGHALSDDLNGYRQEGFGDLEMTVRDGVRCSAALAYLAPAVSRSNLTVRSGS
                     PVQRLNVESGRCEGLTYRHRGATHTAVAREEVVLAAGAIGSPQLLMLSGIGPGEALEP
                     HGIPVRHELPGVGANLMDHLEIYFQQACTQPVSLNRWLNPLGKGVIGARWLLTASGPG
                     ASNHFETGGFVRSAADVPWPDVQFHFLPAAVSYDGRSVADADGFQVHVGPMLSPSRGR
                     LTLRSADPDVAPVISFNYMSCEEDWQVFRRAIRLSRELFAQRAFDPYRGVEIAPGSGA
                     DSDGAIDAFVRRSTESAYHPCGTCRMGNDPMAVVDSECRVRGIQGLRVVDASIFPHIT
                     NGNLNAPTIMTAEKAADAILGRRLAAEALGYFDAPASPTR"
     gene            7196..7978
                     /locus_tag="AC16VR_08705"
     CDS             7196..7978
                     /locus_tag="AC16VR_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011275.1"
                     /translation="MAQADEKPVPRDAGLEFVYDDGFTESERRKLEAWIAEVAGALEV
                     LVGPLPMDVRFRFARHEGAGPVPWANTLRGRRQGVRLYVDPTQPVEAFRNDWTAPHEL
                     SHLVLPFVGREHAWFSEGFASFMQFRVMRIMDVLDDEAMAARYSERLDTAAAAYSQPN
                     RRFVEAVPQLRAEGLYPTIYWGGASYFLEMEERLKRKGASLVGVLAAYLACCRRNEDD
                     LEDLVARLDDLAGSTTFSDGLTGFRRVVGFPPHRHLQALAPR"
     gene            complement(7975..8664)
                     /locus_tag="AC16VR_08710"
     CDS             complement(7975..8664)
                     /locus_tag="AC16VR_08710"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024809.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6011276.1"
                     /translation="MYRLHGATSSPFVRKTWIFMKEKGLEFEHCELDPLDKSQRFLAM
                     NPLGRIPVLEEPDGNFIADSSVICDYLERCHPGPALYPSNNRDRARALWLEEYADTAL
                     NQVCARIFWMHIIIPFRTGRPVDQGEIDAFKEEDYPGVFDYLDRAAPETGDGMVGGRF
                     GIADMALAAPVRLLDLAGDPLDAERWPGFDAHYQRVVARPSAQSIVAKDLATTEVWRT
                     TGNAPASTQSG"
     gene            complement(8702..9646)
                     /locus_tag="AC16VR_08715"
     CDS             complement(8702..9646)
                     /locus_tag="AC16VR_08715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6011277.1"
                     /translation="MPRKTSPDGEGGMMRIGISVSSSYQVDDPREGARNMIERARAAR
                     DAGLDSLFVGDHHVTPFTYYQNTPILGRMLAEWSEMPFGALYLLPLWHPVVLAEQIAT
                     LASIAPGRFIMQCGLGDDRQGKAMGVDMSRRVGMFRASLEVMRALWRGESVDEERYWH
                     LCGARIAPLPPEPVEVWIGALVQPAIRRAAQLGEGWLASPNLTPAQTADAANVYRQAC
                     AEFGRSPAAVALRRDIFVGATSQAARATVTPYVEAGYRGMSEDALLFGSPGEVADQLR
                     SFADQGYTDVIVRNLSARQDESLATIERLAEVKALLTG"
     gene            complement(9624..10184)
                     /locus_tag="AC16VR_08720"
     CDS             complement(9624..10184)
                     /locus_tag="AC16VR_08720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6011278.1"
                     /translation="MADETDTQATNYDDVRQYRLDPEREQELVNTAGECVFCWANKAG
                     HPIGVITAYVPVKGKIWMTATRERVRIKAIARDGRSSVVISSKGTPMGGGKTVTYKGH
                     TVIHDDQDTKMWMYNLLAQGMAASKDEGNEFTKGLDPEMFVRFLDTPERVVLEFTPEM
                     SIPFDGDKMAMGTAAAYAAFAKENQS"
     gene            10301..>10624
                     /locus_tag="AC16VR_08725"
     CDS             10301..>10624
                     /locus_tag="AC16VR_08725"
                     /inference="COORDINATES: protein motif:HMM:NF012575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HIR6011279.1"
                     /translation="MKPVRNKPSYREIIQGDATPVADVLALTANPQQSTEDIPFEHYI
                     SDEFFEREMRKMWPKVWQIACREEHIPEVGDYFVYDIGRYSILVVRSAEDQIKAYHNS
                     CLHRGT"
BASE COUNT         1771 a         3436 c         3540 g         1877 t
ORIGIN      
        1 ctgccgacga gggccacgtt gccgatacgc agccgctcga gcagcgactc gcgatcgtcc
       61 tcttcgtact tcagcgccag catgtgcagg gcctgcatga ccgtgcgggt gccctggctg
      121 tagcccagaa tgtggatccg cctcacgtcg gtgctctgcg caaggaattc cagcaggatg
      181 cgcagctgtc gggcggagac caccgccacc tcggcgtcgg cgaagtaggc gagatagctc
      241 ggcgtggaag gccacgagaa agcgatgaat gccccttcgt agcccaggaa gtgccagagc
      301 tccgtggcca cgagcaccgg gtattcgaac cgcacccggt agccgtgcac gtagatgtag
      361 acgtccttct gctcggactg ggcgagcttg tcgttgacca gcgccgcgaa cgcttcgtcc
      421 gccagcggga aatcgggcgc ttcgatgaat ggctcgatgc tcggctcgag gaacccgagc
      481 tcttccacgc cggtcacgct caaccggtat ccctcgccgc gatcgctcgc catcgacacc
      541 cgaatggctt cttcccaggt gaagccgccc tcgccgagct ggatggaggc gacgcctgcc
      601 cgaaggccct cgccgcgctt gccgacgtag tgcgggtctg cgggatcggg cggctttccg
      661 gcaccagccg gcctgcggtc cgttacgtac agcatgccgc gatacggcaa cgattccggg
      721 ctgaagtcgt cggcgaaggg gctgaagtcc tcgtcctggt aaacggtggg cgcgggcatc
      781 agctccagca cgtagggccc ttttgcagcg catgcggtca gagcagccaa catggccacg
      841 gcagcaaaca agcgcagcga tgggttggct cgctcagtaa tcaaggcttg gcgacctctt
      901 gatggctgac gttttcattc tcgaagaggt caatgatgcc acatcagcac aggtccagta
      961 tgatgcgcgt cgtcgagggc ctgctgattt caggtacgtg gcacgttgtt gggactagcc
     1021 gagcgtagcg agaggacttg cagttatgaa tcttggatgg gtcagcgtgg gacggcattc
     1081 ggtcatcttc ctgatggccg cattgctgat gcttggcagc agcgggcttc gcgcggaaca
     1141 aggcgacgag acctggtggt ccatcacgcc ctacatctgg gccatggata cgaagctgga
     1201 tctcagctgg cgggactcgc ccgtcggcgg caccgagatc gatttcaagg acgcgctggg
     1261 caatctcgat gcggcgttcc aggtggccgt ggaagggggg cggggtcgct ggagcgcctt
     1321 tgcggatgtg accttcgtca agatctcgga cgcgactggt gaggatgcgt tgcggctgaa
     1381 aaccaagaac aagcaggtgt tcctggacgc cggggttgcc ttctggccgg gcggcgccgg
     1441 ctcgccactg agcatctatg gcggagcgcg ctacaccggg ttcgacaaca cctaccgtct
     1501 gcaattccag ggcgacgatg tcgcccgtcg agattcgtcg gccgactact tcgatgctct
     1561 tctgggcgtt cgctaccgtt tcgatctgtc ggatcggtgg tccatcctca ccctcgggga
     1621 cgtaagcttc ggcgaatccg aaggcagctg gcaggctcag gcactggccg cctacacggt
     1681 gggcaagcgg cgcatgaacc ggatcatgtt cgggtatcgc tatcgggaca ccgagttcga
     1741 cgatgccgag ctgaccacgg agtacgggtt ccacgggccc ctcgtcggtt tcaacttccg
     1801 gttctgagac cgccaacggg ttattgacct ccccgcccgg cgcgatggcg cgtagccctt
     1861 gtccgctccg ggacgtttct ggttccatgg gatgcagctg attcatccgc tcttcatggg
     1921 ggagtcctat gtcagactca gaaatcgtcc tctgcgagat tgacgacggc acagccacgc
     1981 tcattctgaa ccggcccgag gtcctcaacg ccatgaacga cgagctcatg ggcggcatct
     2041 gccgggccat ggaacgggtt gtcgaagacg aaagcgtgcg cgtggtcatc ttgacgggcg
     2101 cggggcgcgg cttttgcgcc ggcgcggatc tcgcccgcgc ggcggagggg ccggcagacc
     2161 cggacctgca ggacgccccg gacagcggcg atctcttcaa cggcgcggtc agcgcgctga
     2221 tgagctgccc ggtgccgacc atcgctcggg tcaatggtcc tgcagccggc ggcgggctcg
     2281 ggctggcgct ggcctgcgac gtcgccatcg ccgcggagtc ggcaatcttc gtggcgacct
     2341 tcggccccaa tctcggcatc gtgcccgatc tcggctccac ctggagcata ccgatgaggg
     2401 ttgggcgtgc ccgtgccctg gggatggcgc tgctgggcga tcgcatcacc gcgaaccagg
     2461 ccgaagcctg gggcctcatc tggcgctcgg tgccggatga ggagctcgac gcggaagtcg
     2521 gccgcgtggc gatggttctg aagcgctcct cgccgagcgc gtgcacccgc attcgcgaat
     2581 gcgttgatgc agccattcac cacacctttg gggatcagct tgggctggag atggcgcacc
     2641 agggagtgct catcccgcgt aacatgcagg agggggccag ggcgttcatg gaaaaacgcc
     2701 cccccgagtt cggcggcgat cggggatagc gggaaacgtc agcgggcagg ggcgtggcat
     2761 atataatctt cagatgcgcg acagcgagat tctgcaacgc ctggaaaaac gcctaggcac
     2821 cgcccacgcc cgtgagcgcc tcggcgtgga gacggctcac gagtctcggc tattcagcca
     2881 tgggatccgc ttttttcacc cggagaactg gtactcggtt cattccatca ttcgcaactc
     2941 gctcaaggtc tcgggcctgt atgcccgcgc cagcaggaac gctctcgatc atcgggtggt
     3001 ggagaatcac gtggtgctcg acgctcttcc cgccggtcta gacgggctgc gggtcctgca
     3061 cctctcggat ctgcacgtgg acgtgagcga cgagctgctg cacgccatca tcgaaagggt
     3121 ccgggagctg acctacgacg tctgcgtgct gaccggcgac taccgtttcc gcacctacgg
     3181 cgccatcgac gatgccctgc gcggcatggc ccggctgcgc agcaacctgg acggatcggt
     3241 ctacgccgtc ttcggtaacc acgacagcgt gaccatgctg cccgccctgg aggacatggg
     3301 cattcgcatg ctcatgaacg aaagtcacgc ccaccggcac ggtgacgatc acctctacct
     3361 tgccgggatc gacgatgctc acttcttcgg cgtgcacgac atcgagaagg ctctggccgg
     3421 cgttcccgac acggtgccca ggatcctgct gagccatacg cccgaggtct accggcaggc
     3481 ggcgcgcgcc ggcgtgcagc tgctgttgtg cggccatacc catgggggtc agatctgcct
     3541 gccggggggc tatccggtca cgctcgactc tcgcgttccc aggcgcctcg gacgcggatg
     3601 ctgggattac aaaggcatgc gggggtacac gtcgcccggc gcaggggcat ccatggtcgc
     3661 cgtccgcctg aactgtccgc cagagcttac ggtgcacgtc tttcattcgg cgggccctgg
     3721 cgccgcgcag cttcagtagg gctcgtcgcg gatgccgatc cggtagagta ctttcgacag
     3781 cacgaaatca ccggcgaaga acaacgccac gagcaccgcg atgtcccagc ccgtcagccc
     3841 cagcgccgag ctgcagaccc acatgggcag agcggtttcg gggatctgat ccaggccgcg
     3901 gaaccgaccg ctgggcggaa tgccccggcg ccgcttgacg aagctcgaca gcgcgtcccc
     3961 ggacatcgtc aacgccgagt agatcacacc cagctgccag ccgtagccta gcgccgttgc
     4021 cacgagggtg gtggtggcca ggctcagcac cagcccccgg acggttttgc tcgaccccag
     4081 cagaggccgc ccgtccagaa aggtgagacc gccgtccagg gggatcgccc agcgggcgcc
     4141 gaacaggcgc ttgccgatca ccggagcccc attggcgctc agcgccagcg cgagcacctt
     4201 cagggcaagt atgagatcca gattcatgtg caggtccgtg ctccgctgcg tcaatagatc
     4261 cgcgggacga tccggtaggg aacccggcgc cggtactcgt cccacagcgt cccgtacttc
     4321 tccgcacagc gacggtcgtc atcgcgctcc cgcggcacca gcagggccag atagtacagg
     4381 ggatatagcc agggccaggg gctgtccaga tgcccgaggg acatcgcgag acccgtggcc
     4441 atgaggatct cgcccaggta gttgacgtgt cgggcgacgc cccagaaccc gccgcacagc
     4501 agggccaggt tgccgccgtg aatgacccgc ggaggaaatc ggcccaggaa cgcccgctcc
     4561 gggaatcttt tgaaaacgta cttctgaagg tttgcgcccc gcgccagtac ccagccggac
     4621 agaaaaacga gcgcgctcag cgcgagccag atccagccga ggtcggcgat caccgacggc
     4681 ttgcccaggt gggcggtgcc ccacaggccc acagggtaga agaaggggta gaaggccagg
     4741 cagccccagc cgagcttgag ccccacgcgc tcggcgaaca ggtcgtaggt gtagagatga
     4801 acccgctcga agacgagata gtccagcacg aaaaaagtga gcagagctgc atggagatat
     4861 acgccggggt tgctggcgct acccagggca cccgcgtgaa acgccgtggc ggacagggtg
     4921 ttcaggccga gcagaacggc gcccgcgaga tagagaaaca tcttcgcatc gacacgcttg
     4981 ttgaagtgct gcggattttc cggcctgccg aggtagagat cggctaaaag tgaccggccg
     5041 gtcgggggcg cgccgagcac ggtgatcagg ctgtaggcca gcccgaccag acaggccccg
     5101 cccattgcgc tccagcggtt gacgtagagc cagtcccagc tgatccagcc cttccagcct
     5161 gcagcgagcc agaccgaagt gacgagggcg aacacgacaa ggccgttcag gcggtaggtc
     5221 agaggctggc ccgtgccggg atccagcgcg tacccgtcta ccctccgcgc gggcagggcg
     5281 agatgcagga tcagtatcgc aagataaacc gccagcggcg cgaaccatcc ggccagcagg
     5341 ctaccgctca ccatcttcct tccccggggc gctgacctgg aattctaacc gccgccgcgc
     5401 cgctgtgccc catttccgcc cggcgtctgc tagcctgcac ctcgggcaga cgcgcagcag
     5461 tgactgggca gggtacggga tcgttggaag tcttcgacta catcattgca ggggccggct
     5521 ccgcgggatg cgtgctcgcc aaccggctct ccgaggacgg tgcccgatcg gtgttgcttt
     5581 tagaggccgg tggcagcgat cggcatctgt tcgttcgcat gccttcggcg ttctcgctgc
     5641 ccattggccg gccgcgattc gactggtgct atcaggcgga ggccgagccg gcgctcgacg
     5701 gcagaagcct ggcctgcccg cgggggcggg tgctgggcgg gtcgtcgtcc atcaatggca
     5761 tggtttacgt gcgcggccac ccgcgcgact tcgagcgctg ggagagcctg ggtgctacgg
     5821 gatggggcta cgcggacgtc ctgccctact tcaagcgggc cgagcatgtg ctcggcgctg
     5881 ccgagggaga cccctatcgg ggccgggcgg gaccgctgac cgtcagccgc gggcggcgcg
     5941 ccaatcccct ctacgagacc tttctccagg cggctgagca agcggggcat gcgctcagcg
     6001 acgacctcaa cggttaccgg caggaaggct ttggtgacct ggagatgacg gtgcgcgacg
     6061 gggttcgctg cagcgcggcc ctggcctatc tcgcccctgc tgtctcgcgg agcaacctga
     6121 cggtccgctc gggctccccg gttcagcggc tgaacgtgga atcgggccgc tgcgaaggct
     6181 tgacctaccg gcatcggggg gcaacccaca ccgcggtggc ccgggaggag gtggttctgg
     6241 cggcaggagc catcggctct cctcagctgc tcatgctctc cggcatcgga cccggggaag
     6301 ccctcgaacc tcacgggatt cccgtgcgtc acgagctccc cggggtgggt gccaacctca
     6361 tggaccatct ggagatctat tttcagcagg cgtgcactca gcccgtgtcg ctcaaccgct
     6421 ggctcaaccc gttgggcaag ggggtgatcg gcgctcggtg gctgctcacc gcttccggtc
     6481 cgggggcctc caaccatttc gagaccggcg ggttcgtgcg cagcgcagcc gacgtcccct
     6541 ggccggacgt ccagtttcac tttctgccgg cggccgtgtc ctacgacggg cggtcggtgg
     6601 ccgatgcgga cggttttcag gtgcacgtgg ggcccatgct gagccccagc cgcggccgcc
     6661 tcaccctgcg ctcggcagac ccggacgtcg cccccgtcat cagtttcaac tacatgtcct
     6721 gcgaggaaga ctggcaggtg ttccggcggg cgattcgcct ctcccgggag ctcttcgccc
     6781 agcgggcatt cgacccttat cggggtgtcg agatcgcccc ggggagcggc gccgattcgg
     6841 acggggcaat agatgccttc gtgcgccgca gcacggagag tgcctatcac ccctgcggaa
     6901 cctgccgcat ggggaacgac ccaatggcgg tggtggactc ggagtgccgc gttcgcggaa
     6961 tccaggggct gcgggtcgtg gacgcctcga tcttccctca catcaccaac ggcaacctca
     7021 acgcccctac gatcatgacc gctgaaaagg cggcagacgc cattctcggg cggcgattgg
     7081 ccgcggaagc gctgggttat ttcgacgcgc cggccagccc cacgcgatga tgacgtctcg
     7141 ctggcgtttg cgagtgctgg gcttggtgtt gctcaacggg ataggcgtct gcagcatggc
     7201 ccaggcggat gagaagccgg tgccccggga tgcgggtctc gagtttgtct acgacgacgg
     7261 ctttacggaa tcggagcgcc gcaagctgga agcctggatt gccgaggtgg ccggtgcgct
     7321 ggaggttctc gtcgggcctt tgcccatgga cgtgcgtttt cgatttgccc gtcacgaggg
     7381 ggcgggcccc gttccctggg ccaacaccct gcgcggccgc cgtcagggcg tgcgcttgta
     7441 tgtggacccg acacagccgg tcgaggcgtt tcgcaatgac tggaccgcgc ctcacgagct
     7501 cagccatctg gttctgcctt tcgtcggccg cgagcatgcc tggttttccg agggcttcgc
     7561 cagcttcatg cagttccgcg tcatgcggat catggacgtg ctggacgacg aggcgatggc
     7621 tgcgcgctac tcggaacgtc tcgacacggc tgccgccgcc tacagccagc cgaaccgccg
     7681 gttcgtcgag gcggttcccc agctgcgtgc cgaggggctc tacccgacca tttactgggg
     7741 tggcgccagc tactttctcg agatggagga gcgcttgaag cgcaagggcg cctcgctcgt
     7801 cggggtgctt gcggcctatc tggcctgttg ccggcgcaac gaggacgatc tcgaggacct
     7861 ggtggccagg ctcgatgacc tggcgggctc gacgacgttc tccgacgggc tgaccggctt
     7921 tcgccgtgtc gtggggtttc caccccaccg gcatctccag gcgctcgctc cccgttagcc
     7981 gctctgcgtg gaggcgggcg cgttgccggt ggtccgccac acctcggtgg tggccagatc
     8041 cttggcgacg atggattgcg ccgagggccg cgcaacgacg cgctgatagt gcgcgtcgaa
     8101 gccgggccag cgctcggcat ccagcgggtc gcccgccagg tcgagcagcc gcacgggtgc
     8161 ggccagcgcc atgtcggcga tgccgaaccg gccgcccacc atcccgtcgc ccgtctccgg
     8221 cgctgcccgg tccagatagt cgaacacccc cgggtaatcc tcttccttga aagcgtcgat
     8281 ctcgccctgg tctaccggcc tgccggtacg gaaaggaatg atgatgtgca tccagaaaat
     8341 ccgcgcgcac acctggttca gcgccgtgtc ggcatactcc tcgagccaca gcgcccgcgc
     8401 gcggtcgcgg ttgttcgagg ggtagagcgc gggcccggga tggcaccgct ccaggtagtc
     8461 gcagatcacg gacgagtcgg cgatgaagtt cccatcgggc tcctccagca ccgggatccg
     8521 gcccagaggg ttcatggcta gaaagcgttg ggacttgtcc agagggtcta gctcgcaatg
     8581 ctcgaactcg agcccttttt ccttcatgaa gatccacgtc ttgcgcacga acggtgagct
     8641 ggtggcgccg tgaagtctgt acattgaaac gtcctctcgg gtcggtcgtg ctcttcgggc
     8701 gtcagccggt cagcagagcc ttcacctcgg ccagacgctc gatggtggcc aggctttcgt
     8761 cctgccgggc gctgaggttg cgcacgatga cgtcggtgta tccctgatcg gcaaaggacc
     8821 tcagctgatc ggccacctcg ccaggagagc cgaacagcag ggcgtcctcg ctcatgcccc
     8881 gatagccggc ctccacgtaa ggggtgacgg tggcgcgcgc ggcctggctg gtcgcgccga
     8941 cgaatatgtc tcggcgcagg gcgacggcgg caggcgatct gccgaactcc gcgcaggcct
     9001 gccggtagac gtttgccgcg tcagcggtct gggcgggggt gaggtttggc gaggccagcc
     9061 atccttcgcc aagctgggcg gcgcgccgta tggcaggctg caccagagcg ccgatccaga
     9121 cctccacggg ctccggcggc agcggggcga tgcgggcgcc gcagaggtgc cagtagcgtt
     9181 cctcgtcgac gctctcgccc cgccaaaggg cgcgcatgac ttcgaggctc gcacgaaaca
     9241 tgcccacccg tcgggacatg tccaccccca tggccttgcc ctgccggtca tcgcccagcc
     9301 cgcactgcat gatgaagcgc cccggcgcga tgctcgccag ggtggcgatc tgctctgcca
     9361 gcacgacggg gtgccacagg ggcagcaggt agagcgcgcc gaacggcatc tcgctccact
     9421 cggccagcat gcgtcccagg atcggcgtgt tctgatagta cgtgaacggc gtgacgtggt
     9481 ggtcgccgac gaacagggag tcgagccccg cgtccctggc ggcacgggcg cgctcgatca
     9541 tgttgcgcgc gccctcgcgg gggtcatcaa cctggtagct ggaagagacc gatatgccga
     9601 tgcgcatcat gccgccctcg ccgtcaggac tggttttcct tggcaaacgc cgcgtacgcc
     9661 gccgccgtgc ccatggccat cttgtcgccg tcgaagggaa tggacatctc cggggtgaac
     9721 tccagcacga cgcgttcggg agtatccagg aagcgcacga acatctccgg gtccagcccc
     9781 ttggtgaact cgttgccctc gtcttttgac gctgccatgc cctgggccaa gaggttgtac
     9841 atccacatct tggtatcctg gtcgtcgtgg atgaccgtgt ggcctttgta ggtaaccgtc
     9901 ttgccgccac ccatgggggt gcccttgctc gagatcacca ccgatgaccg gccgtctctg
     9961 gcgatggctt tgatgcgcac gcgctcgcgg gtggccgtca tccatatctt gcctttcacg
    10021 ggcacgtagg ccgtgatcac gccgatgggg tgacccgctt tgttcgccca gcagaagacg
    10081 cactcgccgg cggtgttgac cagctcctgc tcgcgttccg ggtccagccg atactgccgg
    10141 acgtcgtcgt agttggttgc ctgggtatcg gtttcatcgg ccatcgtggt gctccctttc
    10201 aacgtgcctt cgccccggtg tgttcgacgc ttgatgcgtg gttggccggc aagtataacg
    10261 gtttgcacat cggctcgatg ggccgtatgg ggaggacatc atgaagcctg tgcgtaacaa
    10321 gccaagttac cgggagatca ttcaaggcga tgccacgcct gttgcggacg ttctcgcgct
    10381 cacggcgaac ccgcagcaga gcacagaaga cattccgttc gagcattaca tctcagacga
    10441 gttcttcgaa cgcgagatgc gcaagatgtg gcccaaagtc tggcagatcg cctgccgcga
    10501 ggagcacatc cccgaggtgg gcgattactt cgtctacgac atcggccgct actcgatcct
    10561 ggtggtgcgc agcgcggaag atcagatcaa ggcgtaccac aactcctgcc tgcatcgcgg
    10621 cacc
//
LOCUS       DBNHQR010000383         4113 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_921903, whole genome shotgun sequence.
ACCESSION   DBNHQR010000383 DBNHQR010000000
VERSION     DBNHQR010000383.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_921903"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..2097)
                     /locus_tag="AC16VR_08730"
     CDS             complement(97..2097)
                     /locus_tag="AC16VR_08730"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012612.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HIR6011280.1"
                     /translation="METAPKASALHPEARVVEYRLTIAEQTLSPADRKVQALTINGGT
                     PGPVLRLREGDVARITVVNGLADDSTSIHWHGLLVPNIEDGVPVVTTPVIGPGQSRTF
                     EFLVRQHGTYWYHSHTGHQEQRGVYGAIVIEPKTETKAADHDEVVILSDWTDEDPRDV
                     QRSLLRGSDWYSFRKGTSQSLLGAYQAGKLGEYLDREKALVPPMDLSDVAYDAFLMNG
                     RRRLELPGKPGETVRVRLINAGAASYFYLDAATGPLRIVAADGQDVKPFDQKRLLIGP
                     AETYDVLVTVPAQGAWELRSTAQDGSGSVSAWVGSGERHEATPPPAPDRYGMAAYLTS
                     ILDQIDPEPGAKEQERPISPYAKMRAAQPHPVASSHRELTLKLTGDMIRYEWSFDGLD
                     PREAEAIKVKEGETIRVRLVNNTMMHHPIHFHGHFFRLVDANDKDPATSPLKHTVDVP
                     PMSVRTIEFTANEGQGDWLIHCHLLYHHITGMVRTIRVTSAEGAEPPHAAATHSMATT
                     YAWGQASLTTAHFAGFATLQSGRDNWYLRWDEGVRAGDWHETEALYQRYVDPRLALMA
                     GYRSDTSAGGREAAFAGATFRLPYFLQLTLTQQAGGETRGELTKSLQLSDRFSLDLAL
                     RHGRKTGTFTSADLNYLLNKTFSLTAGYSSDFGASAGILIRF"
     gene            2323..2562
                     /locus_tag="AC16VR_08735"
     CDS             2323..2562
                     /locus_tag="AC16VR_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011281.1"
                     /translation="MSKDSIDELLSAWEVSPRRREAFSSQVIAGRPRGPLVRVSAAVL
                     AVFLVGGLAGWRANTDDTRQMAKAHARSIVEASFR"
     gene            2559..3008
                     /locus_tag="AC16VR_08740"
     CDS             2559..3008
                     /locus_tag="AC16VR_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011282.1"
                     /translation="MRTRLLLTIVLASLLAGYAGWWVARDFRKAEACERGSELGWLQA
                     EFRLDDTAVARIGQLQRRFEEECEVHCEQVRQARALMATKPGDESKVRLEAALGKCQA
                     SRREHVLAIAACMPPEKGQAYVDLIMPQVEALSHEGAPGLDGHHKAK"
     gene            3005..3511
                     /locus_tag="AC16VR_08745"
     CDS             3005..3511
                     /locus_tag="AC16VR_08745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR6011283.1"
                     /translation="MSLLPAKDDQAGWRQLFDEWNAPIRGYLHRLLPDSADAEEVAAE
                     AFATAWRTSDRFTGGNVSTWLFGIAINLARNRRRSWLRRLARFTGLTPEIPEGSVEDS
                     ADVDERAALVRAAVQRLPEGLREPLILTAYSGMSHQEAGEALGLTAKAVERRVASARA
                     RLRSDLII"
     gene            3614..>4113
                     /locus_tag="AC16VR_08750"
     CDS             3614..>4113
                     /locus_tag="AC16VR_08750"
                     /inference="COORDINATES: protein motif:HMM:NF015763.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035444 - nickel cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HIR6011284.1"
                     /translation="MDFSQLLQQGTAHAWLYIPVAILLGALHGLEPGHSKTMMAAFII
                     AVRGTAWQAALLGFCAAVSHTLIIWILAALALNYGSRWNVEQAEPYFLMVSGVIVVAM
                     AVWTIMRLRADKAHAHHHHHDESRRLRTADGELVLDIYEAGIPPVFRVAFPERSGLAP
                     VSVRTIR"
BASE COUNT          636 a         1260 c         1459 g          758 t
ORIGIN      
        1 ggtagggttt ggccgcgctt tcgacggcga cggcggtgat gctggccagg gcggccagca
       61 ggatgacgag catggtgttt ttcatggttg tgtgggtcag aagcggatga gaatcccggc
      121 gctcgccccg aagtcggagc tgtagccggc ggtgagcgag aaggtcttgt tcagcaggta
      181 attcagatcg gccgaagtga aagtacccgt cttgcgcccg tggcgcaagg ccaggtcgag
      241 ggagaagcgg tcggacagct ggagcgactt cgtcagctcg ccacgggttt ccccgcccgc
      301 ctgctgggtg agcgtcagct gaaggaagta ggggagccgg aaggtcgccc ccgcgaaagc
      361 ggcttcgcgg ccgccggcgg acgtatccga ccgatagccg gccatcagcg cgagccgggg
      421 gtccacgtag cgttggtaga gggcctcggt ctcgtgccag tctccggcgc gcacgccctc
      481 gtcccagcgc aggtaccagt tgtcgcgtcc cgactgcaac gtcgcgaaac cggcgaaatg
      541 ggcggtcgtc agggaggcct ggccccatgc gtaggtcgtg gccatcgagt gcgtggcggc
      601 ggcatgggga ggctcggctc cctcggccga ggtgacacgg atggtgcgga ccatcccggt
      661 gatgtggtgg tagagcaggt ggcagtggat cagccaatcc ccctggccct cgttcgcggt
      721 gaattcgatc gtgcgcacgc tcatcggcgg cacgtcgacc gtgtgcttca gcggggaggt
      781 ggccgggtcc ttgtcgttcg catctaccag ccggaagaag tgcccgtgga aatggatcgg
      841 gtggtgcatc atggtgttgt tcaccaggcg cacgcggatg gtctcgcctt ccttgacctt
      901 gatcgcctcg gcctctcggg ggtcgagtcc gtcgaagctc cactcgtagc ggatcatgtc
      961 gcccgtgagc ttgagcgtga gctcacggtg ggaggatgcc acgggatggg gctgcgcggc
     1021 tcgcatcttg gcgtaagggg agatggggcg ttcctgctcc ttggcgccgg gttccgggtc
     1081 gatctggtcg agtatggagg tcaggtaggc ggccatgccg taacgatcgg gcgccggcgg
     1141 gggcgtggct tcgtgccgct cacccgagcc gacccaggcc gagaccgagc cgctgccgtc
     1201 ctgggccgtg gatcggagct cccatgcgcc ttgggcgggc acggtgacga gcacgtcata
     1261 ggtctcggcc ggaccgatca gcagccgctt ctggtcgaag ggcttcacgt cctgcccgtc
     1321 ggcggcgacg atgcgcagcg ggccggtcgc ggcgtccaga tagaagtacg aagccgcgcc
     1381 ggcgttgatc aggcggacgc ggacggtctc gccaggcttg ccgggaagct ccaggcgacg
     1441 ccggccgttc atcaggaacg cgtcgtaggc cacgtcggaa aggtccatcg gcggaacgag
     1501 cgccttctcg cggtcgaggt attcgccgag cttgccggct tggtaagcgc cgaggagcga
     1561 ttgggacgtg cccttgcgga acgaatacca gtcgctgccg cgaaggaggg agcgctgcac
     1621 gtcgcgcggg tcctcgtcgg tccagtcgga gaggatgacg acctcgtcgt gatcggcggc
     1681 cttcgtctcg gtcttgggct ctatgacgat ggcgccgtac acgcctcgct gctcctgatg
     1741 tcccgtgtgg ctgtggtacc aataggtgcc gtgctgccgc acgaggaact cgaaggtgcg
     1801 ggactggccc gggccgatga ccggggtggt cacgaccggc acgccatcct cgatgttggg
     1861 caccagcagg ccgtgccagt ggatagaggt ggagtcgtcg gctagcccgt tgaccaccgt
     1921 gatgcgtgcg acgtcgccct cgcgtagccg aagcaccggg ccgggggtgc ctccgttgat
     1981 ggtcagggcc tggaccttgc ggtcagctgg gctgagcgtc tgctcggcga tcgtgaggcg
     2041 gtactcgacc acccgggcct cggggtgcag tgcagaggcc ttgggcgctg tctccatggc
     2101 gtcgaagtag gccttacggc agatttcgca ggcctgcgcg taggacgcta tggcgactga
     2161 ggcgaccatg agtccccagg tcgaggtcct tccggctttg cgagcttggt gggcgagcat
     2221 gcaagtacat gtccgcgtcc cccggcatcc cccctgatga aataccccgt gccaaaaaga
     2281 tttagggggg acagggccgg gtccggacac tagaaaggta ccatgagcaa ggattccatc
     2341 gacgagttgt tgtccgcttg ggaggtttcc ccccgtaggc gcgaggcttt ttccagccag
     2401 gtcatcgcag gtcggccgcg tggcccgctg gtccgcgtct ctgccgccgt actggcggtt
     2461 ttccttgtcg gcggcttggc cggctggcgg gccaatacgg atgacacccg tcagatggcg
     2521 aaggcccacg cccggtcgat cgtcgaggct tccttccgat gaggacgcga ctgctgctga
     2581 ccatcgtcct ggcctcgttg ttggcaggtt atgccggatg gtgggttgcg cgtgacttca
     2641 ggaaggccga ggcctgcgag cgcggctccg agctcggctg gctccaggcc gaattcaggc
     2701 tagacgatac ggctgtcgcc aggatcgggc agctccagcg tcgtttcgag gaggagtgcg
     2761 aggtgcactg cgagcaggtt cgccaggctc gtgccctgat ggcgacgaag cccggcgacg
     2821 aatctaaggt ccggctcgag gccgctctag gcaagtgcca ggcttcccgt cgcgagcacg
     2881 tccttgcgat cgcggcctgc atgcctcccg aaaagggtca ggcctacgtt gatctcatca
     2941 tgcctcaggt cgaagcactt tcccatgagg gggcgcctgg ccttgatggg caccataagg
     3001 ccaaatgagt ctgctgcctg ccaaggacga tcaggctggg tggcgccagc tattcgatga
     3061 gtggaacgcc cctatccgag gttatctcca ccggctgctg cctgattcgg cggacgccga
     3121 ggaggtcgcc gccgaggcct tcgccacggc ttggcgcacc tcggaccggt tcaccggcgg
     3181 caacgtctca acatggctgt tcggcatcgc gatcaacctg gctaggaatc gccggcgcag
     3241 ttggctccgc cggctggcta ggttcaccgg cctgacgccc gagatcccgg agggttccgt
     3301 cgaggacagc gccgacgtgg acgagcgcgc cgcgctggtg cgcgccgcgg tgcaacgcct
     3361 gcccgaggga ttgcgcgagc cgctgatcct gacggcgtat tcaggcatgt ctcatcagga
     3421 agcgggggag gccttggggc taaccgccaa ggcggtcgag cgacgtgtcg catcggcacg
     3481 cgcgaggttg aggtctgatc tgatcatctg aaaaactcgg gcgcccgcag gggcctgtgg
     3541 cccttgggca acaccggctt ggtgcggcat tctcggcggt ggacaaatgc catagtcagg
     3601 ataggcatag tgcatggact tctcccaact tctgcagcag ggaaccgccc acgcctggct
     3661 ctacattccc gtggcgatcc tgctcggggc cctgcatggc ctcgagcccg gacattccaa
     3721 gacgatgatg gcggccttca tcatcgccgt gcgtggcacc gcctggcagg cggcgcttct
     3781 cggtttctgc gcggccgttt cccacacgct gatcatctgg atccttgccg cgctcgcctt
     3841 gaactacggc tctcgctgga atgtcgagca ggccgagccc tacttcctca tggtgagcgg
     3901 ggtgattgtc gtggcgatgg ccgtctggac gatcatgcgg ctccgggccg acaaagccca
     3961 tgcgcaccat caccaccatg acgagagccg ccgactcagg acggccgatg gcgagctggt
     4021 cctggacatc tatgaggccg gcatcccgcc ggtgttccgc gtcgcctttc ctgagcgttc
     4081 aggattggcc ccggtttccg tgcgcaccat ccg
//
LOCUS       DBNHQR010000384         3833 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_732516, whole genome shotgun sequence.
ACCESSION   DBNHQR010000384 DBNHQR010000000
VERSION     DBNHQR010000384.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_732516"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="AC16VR_08755"
     CDS             complement(<1..500)
                     /locus_tag="AC16VR_08755"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013134.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR6011285.1"
                     /translation="MRYESPASTKEAVALLAQEKGSAFVLAGGTDLLVRMKAELIEPD
                     LVVDIKRIPAMTDIRVTEKGFRIGAAVSAAQLGRHKALCKAWPGVVEAANLIGSDQIQ
                     GRCTVVGNLCNASPAADSVPALVAAGAKAVIVGRNGRRTVPVEKVITGPGRTSLEKGE
                     VIEALTL"
     gene            799..2649
                     /locus_tag="AC16VR_08760"
     CDS             799..2649
                     /locus_tag="AC16VR_08760"
                     /inference="COORDINATES: protein motif:HMM:NF023649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HIR6011286.1"
                     /translation="MPQREVIEGLYSGATYSPYAERSFPSEVFWGETHLHTGLSLDAG
                     LFGNILGHEDAYRLARGEQITSSSGLPIKLGRPLDWLVIADHSDMMGIATDIQRGAPN
                     IRAVPKGREWSDGFKQGGQAAAEAAFDLIQHFAQFKVPPELVEQYSPGSPIYSGVWDS
                     IIETADRFNDPGRFTAFIGFEWTSVPKGFNLHRNVVLRDDGRRASQVMPLTTQPPLGT
                     TDPLDLYQWLEDYESKTGGQALALAHNGNLSNGWMFPMGKTYAGGRVNKEYVQLRAKW
                     EPLYEVTQIKGDGEAHPALSLNDEFADYGTWDKGNLDLSELKKPEMLSGEYAREALKN
                     GLVLEEKFGTNPYKFGMVGATDSHTALPTAEEDNFFGKSTSVEPSATRVAHPFVASEL
                     ATIEGYELVAAGYQGVWATENTRAALFDAMERKETYATTGPRMIVRFFGGWDYNENDL
                     RSRAPAFAGYEKGVPMGGDLTAAPDGKSPTFMAYAVRDPVGANLDRLQIIKGWLDERG
                     KTHEKVYDVTWSGGRQLDANGKLPAVGNTVDIEAANWTNTIGASELAAVWTDPDFDAQ
                     ERAFYYVRVLEIPTPWWALYDKVRLGAEIPEGVELTHQERAYTSPIWYTP"
     gene            2667..3413
                     /locus_tag="AC16VR_08765"
     CDS             2667..3413
                     /locus_tag="AC16VR_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009868041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047661 - amino-acid racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HIR6011287.1"
                     /translation="MSKRVKILVPIPMDAAGVANRASQLPADRIRDGYEVEFVAVSWG
                     AALGDSYYDTMLMDFSVFEAGLKAQEEGFAAICIDTVSDSGLYALRSRLDIPVFGPGQ
                     AALHAACMLGHKFSIITMWDEWFHLYTKILKEYVLEQRCASLRSIKTRPDLEELLAGK
                     EEVIFKKLEEESLRAIEEDGADVIVLGSTTMHQSHAHLESVLPVPVINPGLLCYKFCE
                     VFLELGLSHSKRAFPSPEAPNDASVLGRHH"
     gene            3447..>3833
                     /locus_tag="AC16VR_08770"
     CDS             3447..>3833
                     /locus_tag="AC16VR_08770"
                     /inference="COORDINATES: protein motif:HMM:NF024092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6011288.1"
                     /translation="MADIPYEYYVDIVTKRYFHGVDKCDMDMVLDCFHEDAVLTEVTS
                     MTVHEGRDEGIRRMFEGLFDAHSRIWHGNFVHTADVDGEAICSQFSVEVTPKETGTEL
                     RYENCNRFYLDNGKFSRVFVYMSGENL"
BASE COUNT          722 a         1188 c         1217 g          706 t
ORIGIN      
        1 agcgtgaggg cttcgatgac ctcgcctttt tccagggagg ttctgcccgg cccggtgatc
       61 accttctcca ccggtaccgt tcttcgtccg ttccggccca cgatgacggc ctttgccccg
      121 gcggcgacca gggccggcac gctgtcggcc gcgggcgatg cgttgcacag gtttccgacc
      181 accgtgcagc gcccctgaat ctgatccgag ccgatgagat tggccgcctc caccacgccc
      241 ggccacgcct tgcacagggc tttgtgccgg ccgagctggg cagcggaaac ggccgctccg
      301 atccgaaacc ccttctcggt cacccggatg tcggtcattg ctggaatgcg tttgatgtcg
      361 acaacgaggt cgggttcgat cagctcggcc ttcatcctga ccagcaggtc ggtgccgccc
      421 gccagcacga aggctgagcc tttttcctgg gccagcagcg cgacggcttc tttcgtcgag
      481 gctggggatt cgtatctcat cgtgagtgtc ctgtggctga agtcggtgag ggatcagacg
      541 atctccgcat cctatccgcg gcgccgcctt ccggcaacga tctcgtcttc gagcgaagct
      601 gattgaccgg gatcgattca ttcgtccaca cgaatcgtct accattcgag catcggggga
      661 aaacgtcagt cggcgcgcgc tggctgcagg ctgaaaaaaa tttaacacgc cctcgatgag
      721 gagtccggca tgacacgtcc tatcgcactg tatttgttcc ttctggcacc tctgacggca
      781 tcggcctcgg acatcggaat gccgcaaagg gaggtcatcg aagggctcta ctctggggcc
      841 acatactcgc cttacgccga gcgctcgttc ccgagcgagg tcttctgggg agagacccac
      901 cttcacaccg gcctgtctct cgacgctggg ctgttcggaa acattctggg ccacgaagac
      961 gcttatcgcc tggctcgagg cgagcagatc acgtcctcga gtggtctgcc catcaagctg
     1021 ggccgacccc tcgactggct ggtgatcgcc gaccactccg acatgatggg catcgccacc
     1081 gacattcagc gaggcgcccc caacattcgc gcggtgccga aaggccgcga gtggagcgac
     1141 gggttcaagc agggggggca ggcggctgcc gaggccgcgt tcgatctcat tcagcacttt
     1201 gcgcagttca aggttccccc ggagctggtc gagcagtatt cccctggttc gcccatctat
     1261 tccggcgtgt gggacagcat catcgagacg gccgaccgct tcaacgatcc cgggcgtttc
     1321 accgctttca tcgggttcga atggacgtcg gttcccaagg gtttcaacct gcatcgcaac
     1381 gtggtgctgc gagacgacgg ccgacgggcc agtcaggtga tgcccctgac cacccagccg
     1441 ccgttgggga ccaccgaccc tctggatctc taccagtggt tggaggacta cgagagcaaa
     1501 accggcggtc aggcgctggc tctggcgcac aacgggaacc tgtccaacgg ttggatgttt
     1561 cccatgggca agacatacgc gggcggccgc gtgaacaagg agtacgtgca gctgcgcgcg
     1621 aagtgggagc cgctttacga ggtcacccag atcaagggag acggcgaagc gcacccggcc
     1681 ctctcgctca acgatgagtt cgccgactac ggcacatggg acaagggcaa cctcgatctg
     1741 tccgaactca aaaagcctga aatgctgagc ggcgagtatg ctcgcgaggc gttgaagaac
     1801 gggctggtgc tcgaggagaa attcggcacc aacccttaca agttcggcat ggtcggggcc
     1861 accgacagcc ataccgccct tcccacggcg gaggaggaca acttcttcgg caagtcgacc
     1921 agcgtcgagc cgtcggcgac tcgtgtcgcc catcctttcg tagcgtcgga gctggccacc
     1981 atcgagggct acgagctggt cgccgccggc taccagggtg tgtgggcaac cgagaatacg
     2041 cgcgccgcgc tgttcgacgc catggagcga aaagagacct acgccaccac gggtccccgc
     2101 atgatcgtgc gcttcttcgg tggctgggac tacaacgaga acgacctgcg cagccgggcg
     2161 cccgcctttg ccggctacga gaaaggcgtg cccatggggg gggatctgac agcggcgccg
     2221 gacggcaagt cgccaacttt catggcctac gcggtccggg atcccgtggg ggccaacctc
     2281 gatcgcctgc agatcatcaa gggctggctc gacgagcgcg gcaagaccca cgagaaggtc
     2341 tacgacgtca cctggtccgg cggccgtcag ctggatgcca acgggaagct tcccgccgtg
     2401 ggaaacacgg tggacatcga agcggccaac tggaccaaca ccatcggcgc ctcggagctg
     2461 gccgccgtct ggacggaccc tgacttcgat gcgcaggagc gcgccttcta ttacgtgcgg
     2521 gtgctggaga tccctacgcc gtggtgggcg ctttacgaca aggtgcgcct cggcgccgaa
     2581 attcccgagg gcgtggagct gacgcaccag gagcgggcgt atacctcgcc catctggtac
     2641 acgccctgag ccagaaggag tcgagcatga gtaagcgggt caagattctg gtgccgatac
     2701 cgatggacgc ggcgggcgtt gccaaccggg catcgcagct gccggccgat cgcattcgcg
     2761 atggttacga ggtggagttt gtggcggtca gctggggcgc ggcgttaggg gattcctact
     2821 acgacacgat gctcatggat ttcagcgtgt tcgaggcggg cctgaaggct caggaggagg
     2881 gcttcgccgc catctgcatc gatacggtga gcgactccgg cctttatgcg ctgcgctcgc
     2941 ggctcgacat tccggtcttt ggccccggcc aggcggcgct tcatgcggcc tgcatgctgg
     3001 gccacaagtt ctcgatcatc acgatgtggg acgagtggtt tcatctctac acgaagatcc
     3061 tcaaggagta cgtgctcgag cagaggtgtg cctcgctgcg ttcgataaag acccgtcccg
     3121 atctggagga gcttctggcc gggaaggagg aagtgatctt caagaagctg gaggaggaga
     3181 gcctgcgggc catcgaggag gacggcgccg acgtgatcgt gcttggatcc acgaccatgc
     3241 atcagtctca cgcacacctc gaaagcgtgc tgccggtgcc ggtgatcaac cccggcctgc
     3301 tctgctataa gttctgcgag gtctttctgg agctcggcct cagccacagc aagcgggcct
     3361 ttccgtcgcc ggaggcgccc aacgacgcct cggtgcttgg ccggcaccac tgaacgagct
     3421 cggtaaaacc aagggaggtg aaccccatgg ccgacattcc ttacgagtac tacgtcgata
     3481 tcgtgaccaa gcgatacttt catggtgtcg acaaatgcga catggatatg gtgctggact
     3541 gctttcacga ggacgcggtg ctcaccgaag tgacctcgat gacggtccac gaagggcggg
     3601 acgagggtat ccgcaggatg ttcgaaggcc tcttcgacgc ccacagccgc atctggcacg
     3661 gtaatttcgt ccacacggcg gacgtcgacg gcgaagccat ctgctcccag ttctccgtgg
     3721 aggtcacccc caaagagacc ggaacggagc tgcgctacga gaactgcaac cgcttctatc
     3781 tggacaacgg caagttctcc agagtgttcg tttacatgag cggcgagaac ctg
//
LOCUS       DBNHQR010000385         1535 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_543149, whole genome shotgun sequence.
ACCESSION   DBNHQR010000385 DBNHQR010000000
VERSION     DBNHQR010000385.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_543149"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1413
                     /locus_tag="AC16VR_08775"
     CDS             <1..1413
                     /locus_tag="AC16VR_08775"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR6011289.1"
                     /translation="RRCGQLVAVLRELGIQRGDRVAILAGNCNQYLETYVGVPAGGMV
                     VVPLNTRHAQPELEYALKDSGARVLLTDRDPGALAAAVDHVISIPDSYESHLAHAEPA
                     GLGEGVSEADLAGLFYTGGTTGLSKGVMLTHRNLIANTFNWLACSPHYSSDRTLIMAP
                     LFHAAGSNGVLGAIWNGGCQVTLGAFDPASALDLIEAHAITETLGVPTMLAAIAEEQH
                     ARPRKTDSLRLIAHGGSPIATEVIRRTMSAFPTASMMEVYGATELSPLATVLVGEEKL
                     VDDLRARSCGQALIGNEIRILNPDGQTVDTGEVGEVVVRGPNVMEGYWNKAEQTAAVL
                     KDGGYWTGDLGYMDDRGYLFLVDRSKDMIVSGGENVYCTEVEEILYQHPAVLEAAAFG
                     VPDEKWGEAVHAVVVPRDEFADLNPSEIIDFCREHIAGYKVPKAVDIRSEPLPKSGPG
                     KVLKRELRAPFWEGQDRGVH"
BASE COUNT          276 a          468 c          517 g          274 t
ORIGIN      
        1 cgccgctgtg gccagctcgt tgctgttctg cgcgagctcg ggattcagcg cggcgaccgg
       61 gtggcgatcc tggcgggcaa ctgcaaccag tatctcgaaa cctacgtcgg ggtccccgcg
      121 ggcggcatgg ttgtggtgcc gctgaatacc cgtcatgccc agccggagct cgaatacgcg
      181 ctcaaagatt ccggcgcccg cgtgctgctg accgaccgcg atccgggggc gctggccgcc
      241 gccgttgatc atgtgatcag catccccgac tcctacgagt cacatcttgc tcacgccgag
      301 ccggccgggc tcggagaagg cgtgagcgag gcggatctgg ccggtctgtt ttacaccgga
      361 ggcacgacgg gactgtccaa gggggtgatg ctcacccatc ggaacctcat cgccaacacg
      421 ttcaactggc tggcctgctc gccgcattac tcctcggatc gcaccctcat catggcgccg
      481 ctgtttcacg cggcggggtc caacggcgtt ctcggggcaa tctggaacgg gggatgccag
      541 gttacgctgg gtgccttcga tccggcgtcg gcgctggatc tgatagaagc ccatgccatc
      601 acggaaaccc tcggggtgcc gaccatgctg gccgccatcg ccgaggagca gcatgctcgt
      661 ccccgcaaaa cggactccct gaggctgatc gcccacggcg gttcgcccat cgccaccgag
      721 gtgatccggc gcaccatgag cgcgttccct acggcgtcca tgatggaggt gtacggcgcc
      781 acggagctct ctccgctggc gacggtgctg gtgggagagg agaagctggt cgatgatctc
      841 cgggcacgct cgtgcgggca ggccctgatc ggcaacgaga tccgcattct caaccccgat
      901 gggcagacgg tggacacagg ggaggtcggc gaggtcgtcg ttcgcgggcc caacgtcatg
      961 gaaggttact ggaacaaggc cgagcagacg gcggcggtgc tcaaggacgg cggttactgg
     1021 acgggcgacc tcggctacat ggacgatcgg ggctatctct ttctggtgga ccgcagcaag
     1081 gacatgatcg tctcaggggg cgagaacgtt tactgcacgg aggttgaaga gatcctctac
     1141 cagcatccgg cggtgctgga ggccgccgcc ttcggcgtgc cggacgagaa gtggggcgaa
     1201 gcggtgcacg cggtggtggt gccgcgcgat gagttcgctg acttgaaccc ctccgagatc
     1261 atcgatttct gtcgtgagca cattgccggg tacaaggtcc ccaaggccgt cgacatccgc
     1321 tcggagccgc tgccgaaatc aggccctgga aaggttttga agcgcgagct cagagccccg
     1381 ttctgggaag gtcaggaccg gggcgttcac tgacacgccc tatgaccggc ccggcggctt
     1441 tatcctagct cttgcggtgc ttcttagttt gcgggaccat agagaagcct caggccaaat
     1501 ttggccaaag aaagaaaaaa cgacggagag tattg
//
LOCUS       DBNHQR010000386         7322 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1239997, whole genome shotgun sequence.
ACCESSION   DBNHQR010000386 DBNHQR010000000
VERSION     DBNHQR010000386.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7322)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7322)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7322
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1239997"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="AC16VR_08780"
     CDS             <1..213
                     /locus_tag="AC16VR_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011290.1"
                     /translation="RADDLPDIRVGFTNTPSPSNPLGIKGCGEAGAIGAPPAVINAIT
                     DALGVRELDMPATSEKIWRVIKEQAL"
     gene            210..1514
                     /locus_tag="AC16VR_08785"
     CDS             210..1514
                     /locus_tag="AC16VR_08785"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase family protein"
                     /protein_id="HIR6011291.1"
                     /translation="MKTLLKNANIIDGLGNAPFAGDVLIDEELILAVGAADGEGATVI
                     DVAGKTVMPGLIDAHCHVTLDETHSNDEMFFHRREGLGALVAAYNVQKLLRAGVTGFL
                     DADCIFDIGVDLRDAIEAGIVPGPRMSTGGNALLTSVGGTAGNLIPAEGRVGYAKVVR
                     SRDEIVTEVRRQIKLGVDWIKVHVTGLVPRQKHRGEIQAWSFDELKTVCDTAHELGIP
                     VVGHCRNAKSIADSVRAGMDMILHATFMDDAALEAVIERKVPLVPTFTFQANLADFGD
                     LVGADPNLREIFRREIAESADMIRRAYEAGVPVLCGTETGFSLTPYGEWHYREMEVFV
                     RDMGFTPLEAIRAATSECGRAIGLQDRVGAVEAGRLADLLVVDGDPSKDVTVLGDKTK
                     IERVFLNGAQLDLPPLPARKPIPGWRVSAYSSSQLFWADAHK"
     gene            1511..2707
                     /locus_tag="AC16VR_08790"
     CDS             1511..2707
                     /locus_tag="AC16VR_08790"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6011292.1"
                     /translation="MSLVTVDSYQAANAALKQKDLRQALYDEGAVIMREVLVNLHGDE
                     HRTRRNVETQVFRRDFFRYYEHEVFPRTLEETIAPFLAAGKMDIVDFGYRVMVNLTAD
                     FTGIDRPKKSPEETEQLIKMLRLFGRTATLAHSTEDREPIRRAALDALADFQRDYVDP
                     SVARRQALIASVNRGDLSEEDLPRDILTVLLKNQDTVQLADDVRVREMAFFALAGAHT
                     SIHTLGHVMHEIFTWCASHPEDWSRFETDPLFVQRAVHESIRLHPSSPIAARRALCPV
                     HVAGKDVQADTRVTVDLQKANRDPAAFGADAERFNPDRPRGAKVPPYGLSFGLGMHAC
                     LGLNLAAGALPKPDTDPDDHHYGTVAMIVRTLLAHRARPEPGSPPRKDTTTARDIWAT
                     YPVTLG"
     gene            2709..3911
                     /locus_tag="AC16VR_08795"
     CDS             2709..3911
                     /locus_tag="AC16VR_08795"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6011293.1"
                     /translation="MDLRITDYRHAHQALCSPHLKQSLYDAGGVIMEDTLLTLHGQRH
                     TRRRHLALRVFRRDYARHYEREVFPKALRDALEPLRGQPSIDVVAFGHAVSMNLTADF
                     AGIDRSEDPEATAALQRLTKKFGEGATLVHSTRAHDQVRAEVREALAEYRGRFLEPSM
                     ARRERLISAWRGSEIPEESLPRDMLTVLLTDRGGLDDDGLAREIAFYLQAGAHSTSNA
                     FAHALWEVLTWLGSDLARRAALAGDALATQRAVHEALRLHPASPVAWRRALEPIDFSW
                     GLHVEPEDHLVVDIAAANRDPEVFGPEADAFQPGRTVLDRRANPFGLTFGIGVHNCLG
                     RDIDAGLPQDSDTEAGSHQYGTVTLLVHHFLSELDAMFDPADLPVPDEDTSRPNWGRF
                     PVRVRWQT"
     gene            3908..4648
                     /locus_tag="AC16VR_08800"
     CDS             3908..4648
                     /locus_tag="AC16VR_08800"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6011294.1"
                     /translation="MSVRRALVTGAAHGIGAAIATRLGSAGYRVGVLDMDGTAAAEVA
                     AALPDALPLTADVTDADSIDRALDAFGEPPNLLVNNAGIVHFGPVVEESVADFVKVIS
                     VNLIGCYVASRQCAQRMSGGDHIISITSINDRTPAPGSGAYPAAKAGVRQLMRQLAVE
                     LAERGIRANSVCPGFIDAGMSAPFYADPKVRETRARAVPLGRLGTAEDVANAVAFLDS
                     EAGAYVNGHELVVDGGVTHTLLKHLPRD"
     gene            4648..5325
                     /locus_tag="AC16VR_08805"
     CDS             4648..5325
                     /locus_tag="AC16VR_08805"
                     /inference="COORDINATES: protein motif:HMM:NF018038.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HIR6011295.1"
                     /translation="MRNVMLIGGIYHPFEEAAPVLARLLESLGIESVITSDVSEAVAE
                     LESADLFTIYALRWRMLNDEKYVPFRKQWAFELPEEDAQAIENFVEAGGGMLGLHTAS
                     ICFDTWYEFPRLLGGSWRWGETFHPPEGNVEVRAVADHPATRGVTDFGLHDEIYHHLA
                     IGNESKVLLEGRVPDGDWHPISWAHEAAAGRVVYSALGHDGRSLGDPHHARFIRQAVA
                     WLLGEDE"
     gene            5322..6083
                     /locus_tag="AC16VR_08810"
     CDS             5322..6083
                     /locus_tag="AC16VR_08810"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF048554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6011296.1"
                     /translation="MRPIAGMSILVTGASSGLGAGAAAYFAERGARVTISARREDRLA
                     AVKEAIGDAAAMVVGDVTSAADRQRMIDEAVRHGGGLDALISNAGNMYRGPITDLDEQ
                     ALLDIFHSNVVAGMLLTGLAVPELEKRQGAVIFVGSIHTMRAFPGASPYAATKGALQT
                     LTRVLAAELGERGIRVNCVIPGAVPTEIYQRAGLLSDEDARARIAGMASMHPLGRVGT
                     EEELAEAMAYLIQAEWTTGAELVVDGGLGLGATNA"
     gene            6157..>7322
                     /locus_tag="AC16VR_08815"
     CDS             6157..>7322
                     /locus_tag="AC16VR_08815"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6011297.1"
                     /translation="MAESQPKPGEARCPGISVQDLMDRDSRTVPAYLREHSYAFLGDE
                     DIPFARYTSHDWFDKEIEHMWPRTWQWACREEHLPAPGDYYVYDVGPYSVIVLRDSSG
                     AIRAYRNACMHRGTKLKPSFSTGSADSIRCPYHGWQWNLEGELQSFPCEWDFPHVDPD
                     AVGLSSVRAELWGGFVFINLDPEAVPLREYLHPLPEHVDSSVFEQRYVAIHIQKELNC
                     NWKIASEAFLEAYHVMETHPQLMRTNGDANTQYDFYGDHVNRLINVAGSPSAYLEEPI
                     TEQEVLDLFLVGDREDIGEALVVPEGGTARQVMAEHFRESVNASGGADLSEVCDSEIV
                     DTIAYFVFPNQQFFSGVSFPIVYRFRPLGMSHDRSLFDFVILKTRPNDGSPAPTA"
BASE COUNT         1258 a         2503 c         2411 g         1150 t
ORIGIN      
        1 cgcgcggacg acctgccgga tatccgggtc gggttcacga atacaccctc gccttcgaat
       61 ccgctcggca tcaagggctg cggcgaggcc ggcgccatcg gagcgccacc ggccgtcatc
      121 aacgcaatca ccgacgctct cggagtaagg gagcttgaca tgcctgccac gtccgagaag
      181 atctggcggg tgatcaagga gcaggcgctg tgaagacact gctgaagaac gccaacatca
      241 tcgacgggct cggcaacgcg ccgtttgccg gcgacgtgct catcgacgaa gagctcatct
      301 tggcggtggg cgcagccgac ggggaagggg cgaccgtcat cgacgtcgcg ggcaagaccg
      361 tcatgccagg cctcatcgac gcgcactgcc acgtgacgct ggacgagacc cattcgaacg
      421 acgagatgtt cttccaccgc cgggaaggcc tcggcgccct ggtggccgcg tacaacgtgc
      481 agaagctgct ccgtgccggg gtcaccggtt tcctcgacgc ggactgcatc ttcgatatcg
      541 gcgtggacct gagggatgcc atcgaggcgg gcatcgtgcc cgggccgcgg atgtcgaccg
      601 gcgggaacgc tctgctgacg tccgtgggcg gcaccgccgg taatctgatt ccggcggagg
      661 gccgcgtcgg ctacgccaag gtagtgcgca gccgcgacga gatcgtcacc gaggtgcgcc
      721 gccagataaa gctcggcgtg gactggatca aggttcacgt gacggggctg gttccgcggc
      781 agaagcaccg cggtgagatt caggcctggt ccttcgacga gctgaagacc gtctgcgaca
      841 ccgcccacga gctcggcata cccgtggtcg gccactgccg caacgcgaag agcatcgcgg
      901 actccgtccg ggcgggcatg gacatgatcc tgcacgccac gttcatggac gacgccgcgc
      961 tcgaggcggt catcgagcgg aaggtcccgc tggtgccgac tttcacgttc caggccaacc
     1021 tcgctgattt cggcgatctg gtgggggcgg accccaacct gcgagagatc ttccggcggg
     1081 agatcgccga gagcgccgac atgatccgcc gcgcctacga ggccggcgtg cccgtgctgt
     1141 gcggcacgga aaccggtttc tcgctgacgc cttacggcga gtggcactac cgggaaatgg
     1201 aggtgttcgt ccgcgacatg ggtttcaccc cgctcgaggc gatacgcgcg gccacgtcgg
     1261 agtgcggtcg cgccatcggc ctgcaggatc gcgtcggcgc ggtggaggcc gggcggctgg
     1321 ccgacctcct ggtagtcgac ggcgacccgt ccaaggacgt gacggtcctc ggcgacaaga
     1381 cgaaaatcga acgggtgttc ttgaacggtg cgcagctgga cctgcccccc ctgcccgcac
     1441 gcaagcccat tcccggctgg cgggtctccg cctactcctc aagccagctt ttctgggcgg
     1501 acgcacataa atgagcctgg tcaccgtcga ctcgtaccaa gccgccaacg cggcgctgaa
     1561 acagaaagat ctgcgtcagg cgctctacga cgagggcgcc gtgatcatgc gcgaggtgct
     1621 cgtcaacctg cacggcgacg agcacaggac ccggcgcaac gtggaaaccc aggtcttccg
     1681 tcgcgacttc ttccgctact acgagcacga ggtttttccg cgcaccctcg aggagaccat
     1741 cgcccccttc ctcgccgccg gaaagatgga catcgtggac ttcggctacc gcgtcatggt
     1801 caacctcacc gcggacttca ccggcatcga ccggccgaag aagtcgccgg aggaaaccga
     1861 gcagctcata aagatgctga ggttgttcgg ccgcacggcc accctcgccc actctaccga
     1921 ggatcgcgaa ccgattcgcc gtgccgcgct ggacgcgctc gcggattttc agcgcgacta
     1981 cgttgaccct tcggtggctc gtcgccaggc gctcatcgcc agcgtgaacc gaggcgatct
     2041 gtccgaggag gacctgcccc gggacatcct cacggtgctc ctgaagaacc aggacaccgt
     2101 gcagctggcc gacgacgtgc gggtaaggga gatggctttc ttcgcgctgg caggcgccca
     2161 tacctcgatt cacaccctag gccacgtcat gcacgagatc ttcacgtggt gcgccagcca
     2221 cccggaagac tggtcgcgct tcgagaccga cccgctgttc gtgcagcgcg ccgtccacga
     2281 atccatccgc ctgcatccct cgagccccat tgccgcccgc cgcgcgctct gcccggtcca
     2341 tgtggcaggc aaggacgttc aggcggacac ccgggtgacc gtcgacctgc agaaggcaaa
     2401 ccgcgacccg gcagcattcg gggcggatgc ggagcggttc aaccctgatc gaccccgcgg
     2461 ggcaaaggtg cccccctacg gactttcctt cgggttgggc atgcacgcct gccttgggct
     2521 caatctggcc gccggcgcgc tgccgaagcc ggacaccgat cccgacgatc accactacgg
     2581 caccgtcgcg atgatcgtgc gcaccctgct ggcccatcgg gcacggcctg agcccggctc
     2641 gccgccgcgc aaggacacca ccaccgcgcg cgacatctgg gcgacctacc ctgtcaccct
     2701 gggctgagat ggacctgcgc atcaccgact accgccacgc tcaccaggcg ttgtgcagcc
     2761 cgcatctgaa acagtccctt tacgacgccg gcggggtgat catggaagac acgctgctga
     2821 ccctgcacgg ccagcgccac acccgccgac ggcacctcgc cctgagggtc ttccgcagag
     2881 actacgcccg tcactacgag cgcgaggttt ttccaaaagc gctgcgcgac gccctggagc
     2941 cgctgcgggg ccagcccagc atcgacgtcg tggcgttcgg ccacgccgta tccatgaacc
     3001 tgacagccga tttcgccggc atcgaccggt ccgaggaccc ggaagccacg gccgcgttgc
     3061 agcggttgac gaagaaattc ggcgagggag cgaccctggt gcattccacg cgcgcccacg
     3121 atcaggtcag ggcagaggta cgcgaagcgc tggccgagta ccgcggccgc ttcctggagc
     3181 cgtccatggc gcggcgagag cggctaatct cggcgtggcg tgggagcgag atacccgagg
     3241 aatccctgcc tcgcgacatg ctcacggtgc tgctgacgga ccggggcggc ctcgacgacg
     3301 acggtctcgc gcgggagatc gccttctacc ttcaggccgg cgcccacagc acgtccaacg
     3361 ccttcgccca cgccctctgg gaggtgctga cctggctcgg cagcgacctc gcccgcaggg
     3421 cggccctggc cggcgatgcg ctcgccaccc agcgggccgt gcacgaggcg ctgcggcttc
     3481 accccgccag ccccgtggcc tggcgccggg cgctcgagcc catcgatttc agctggggcc
     3541 tgcacgtgga gccggaggat cacctggtgg tggacatcgc cgcggccaat cgggacccgg
     3601 aggttttcgg gcccgaggcg gacgcgttcc agcccgggcg cacggtgctg gaccggcgcg
     3661 ccaacccctt cggtctgacc ttcgggatcg gcgtgcacaa ctgcctcggc cgagacatag
     3721 acgccggcct gccgcaagat tccgataccg aagccggcag ccatcagtac ggcacggtga
     3781 cgctcctggt tcatcacttt ctatccgagc tagacgccat gttcgatcct gccgacctgc
     3841 cggtgcccga cgaagacacc tcgcgtccca actggggccg ctttcccgtc cgcgtgcggt
     3901 ggcaaacatg agcgtgcgcc gagcgctggt gaccggcgct gcccacggca tcggcgccgc
     3961 cattgccacc cggctgggaa gcgccggata ccgggtcggc gtgctcgaca tggacggcac
     4021 ggcggccgcg gaggtggcgg ccgcgctgcc cgatgcgctg ccgctgaccg ccgacgtgac
     4081 cgacgccgac agcatcgacc gggcgctgga cgccttcggc gagccgccca accttctggt
     4141 gaacaacgcc ggcatcgtgc acttcgggcc ggtggtcgag gaaagcgtgg ccgacttcgt
     4201 caaggtgata tccgtcaacc tgatcggctg ctacgtggcc agccggcagt gcgcccaacg
     4261 gatgagcggc ggggatcaca tcatcagcat cacctccatc aacgaccgga cgccggcccc
     4321 cggctctgga gcttatcccg cggccaaggc aggggtccgc cagctgatgc gccagctggc
     4381 cgtggagctc gccgagcggg gcattcgcgc caattcggtc tgtcccggct tcatcgatgc
     4441 cggcatgtcg gcacccttct atgcggaccc gaaggtaagg gagacccgcg cccgcgcggt
     4501 tccgttgggc cgactgggca ccgcagagga cgtggccaac gcggtcgcct ttctcgactc
     4561 ggaggccggc gcgtacgtca acggccacga gctggtcgtg gacggcggcg tcacccacac
     4621 cctcctcaag cacctgccga gggactgatg cgcaacgtga tgctcattgg cggcatctat
     4681 catccgttcg aggaggcggc acccgtgctc gcccgactcc tcgaatctct cggcatcgag
     4741 agcgtcatta ccagcgacgt ctcggaggcg gtggcggaac tcgaatccgc cgacctcttc
     4801 accatctacg ccctgcgctg gcgcatgctc aacgacgaga aatacgtgcc cttccgcaag
     4861 cagtgggcat tcgagctgcc cgaggaagat gcccaggcca tagagaactt cgtggaagcc
     4921 ggcggcggga tgctcggact gcacaccgca agcatctgct tcgacacctg gtacgagttt
     4981 ccgaggctgc tcggcggctc ctggcgctgg ggcgagacgt ttcatccgcc cgagggaaac
     5041 gtggaggtgc gcgcggtggc cgatcatccg gcgacgcggg gcgtcaccga cttcggactc
     5101 cacgacgaga tctaccacca cctggccatc ggcaacgaaa gcaaggtact gctcgagggc
     5161 cgggttcccg acggcgactg gcaccccatc agctgggcgc acgaggccgc cgcgggcagg
     5221 gtcgtctact cggcgctcgg ccacgacgga cgatcccttg gcgaccctca tcatgcccgg
     5281 ttcatccgcc aggcggtggc ctggctgctg ggagaggacg agtgaggccc atcgccggca
     5341 tgtcgatcct cgtcaccggc gccagctccg ggctcggcgc cggtgcggcg gcttacttcg
     5401 ccgagcgggg ggccagggtc accatctccg cccgccggga ggaccggctc gcggcggtga
     5461 aggaggccat cggcgacgca gccgccatgg tcgtcggcga cgtgaccagc gcagcggacc
     5521 ggcagcgcat gatcgacgaa gcggtccgcc acggcggggg gctcgacgcg ctgatcagca
     5581 acgccggcaa catgtaccgc gggcccatca ccgacctcga cgagcaggcg ctcctggaca
     5641 tctttcacag caacgtcgtc gccgggatgc tgctcacggg cctcgccgta ccggagctgg
     5701 aaaagcgcca gggcgcggtg atcttcgtcg gcagcatcca caccatgagg gcattccccg
     5761 gcgcttcgcc ctacgcggcg acgaagggcg cgctgcagac cctgaccagg gttctggccg
     5821 ccgagctcgg cgagcgcggc atccgggtga attgcgtgat ccccggcgcc gtgcccaccg
     5881 agatctatca gcgtgcgggc ctgctgtcgg acgaggacgc gcgggctcgc atcgcgggga
     5941 tggcctccat gcaccccctc ggccgggtcg gcaccgagga ggagctcgcc gaggccatgg
     6001 cttacctgat tcaggcggag tggaccacgg gggccgagct ggtggtggac ggcgggctgg
     6061 ggctaggggc caccaacgcg tagcgggcag tggtggcgta tcgtcgccgg tgacttaaaa
     6121 tgtccgctgg ggataaggac aaatcagggg gaggcgatgg ccgagagcca gccaaagccg
     6181 ggtgaggcac gctgcccggg catttcggta caggacctga tggacaggga cagccgaacg
     6241 gtgcccgcct atctgcgcga gcacagctac gcgtttctcg gcgacgagga cattcctttt
     6301 gcccgctaca cgtcgcacga ctggttcgac aaagagatcg agcacatgtg gccgagaacg
     6361 tggcagtggg cgtgtcgcga ggagcacctg ccggcccccg gtgattatta cgtttacgac
     6421 gtcggcccgt actcggtcat cgtgttgcgg gacagcagcg gcgcgatccg ggcataccgc
     6481 aacgcctgca tgcatcgcgg gacgaagctc aagccctcgt tcagcacggg ttcggctgac
     6541 tccatccgct gcccttatca cggctggcag tggaacctgg agggcgagct gcaatcgttt
     6601 ccctgcgagt gggatttccc ccacgtcgat cccgatgccg tgggcctgtc ctccgtccgg
     6661 gccgaactct ggggcggctt cgtgttcatc aacctggatc ccgaggccgt tccgctacgg
     6721 gagtaccttc atcccctgcc cgagcacgtg gacagcagcg tcttcgagca acgctacgtg
     6781 gccatccaca tacagaaaga gctgaactgc aactggaaga tcgcctcgga ggcgttttta
     6841 gaagcctatc acgtgatgga aacccaccct cagctcatgc gcaccaacgg cgacgcgaac
     6901 acccagtacg acttctacgg cgatcacgtc aaccggctga tcaacgtcgc cggctcgccg
     6961 agcgcttacc tcgaggagcc gataaccgag caggaggtgc tggatctgtt tctggtgggg
     7021 gatcgtgagg atatcggcga ggcgctggtg gtgcccgagg gcggcaccgc tcgccaggtc
     7081 atggccgagc atttcagaga gagcgtgaac gcgtccgggg gagcggatct ctccgaagtg
     7141 tgcgattcgg agatcgtgga caccatcgcc tacttcgtct tccccaacca gcagttcttc
     7201 tccggggtat cgtttcccat cgtttaccgc ttccggccgc tcggcatgag ccacgaccgc
     7261 agcctgttcg acttcgtcat cctcaaaacg cggcccaacg acggctctcc agcacccacg
     7321 gc
//
LOCUS       DBNHQR010000387         2997 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1050005, whole genome shotgun sequence.
ACCESSION   DBNHQR010000387 DBNHQR010000000
VERSION     DBNHQR010000387.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1050005"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="AC16VR_08820"
     CDS             <1..486
                     /locus_tag="AC16VR_08820"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6011298.1"
                     /translation="TSRSSGLNATRGPKINAFLQIIRSCQDVMGLFIFSGVFDRHPGL
                     KLVCAEADAGWVPHYMYRMDHAYEYHRYWMKAPPLERKPSEYFRDHVAMTFQDDYVAF
                     QCKDLVNVRRLMWASDFPHSDSTWPRSQSVLAEQTAHLTEQERDWICHDNVAGWYGIT
                     V"
     gene            516..2180
                     /locus_tag="AC16VR_08825"
     CDS             516..2180
                     /locus_tag="AC16VR_08825"
                     /inference="COORDINATES: protein motif:HMM:NF005863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HIR6011299.1"
                     /translation="MAWNFADVFEAIAEISNEEHPACIHSGPGGSAGITISWPELMRR
                     SNRLARYLAAQGLRPDSKVAHYMRNCPAYIETMIASFKGRYVHVNVNYRYRDNELHYI
                     LDNADAEAVVYDAEFREHVAALRPRLPQVRSWIEVASYFEQPADRAAHFAVCYEDVVG
                     NGNDSPLDIERSGNDLLFIYTGGTTGMPKGVMWEHQAIWQAGGSGSSLLNPGQAAPES
                     PREHAQRIANAPLRARMLAACPLMHGTAMLTSINTLSQGGCIVTVPEHSLDPHAMWAT
                     VERYRVNSLAIVGDAFAKPLLRALEERPGAYDISSVFGIVSSGVMWSPEVKRGLLRHH
                     PNMVLMDSFGASEAIGFGTSRTTGDEEAATAKFKIGEHCKVFTEDHRPVQPGSGEPGF
                     IARSGPIPVGYYKDPEKTAKTFPMIDGVRYSVPGDWCLVEPDGTLTLLGRGSVCINSG
                     GEKIYPEEIEEALKTHPDVEDALVVGVPDERWGQAVTGVIVPMDPATFDEAALREHVK
                     SQLAPYKAPKRILISEFPLRAPNGKADYKSVTDFARAALGDTADAK"
     gene            complement(2232..>2997)
                     /locus_tag="AC16VR_08830"
     CDS             complement(2232..>2997)
                     /locus_tag="AC16VR_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6011300.1"
                     /translation="VDATRMSQAFTGSGHIQAIVQDQVNEFVSRQRAATSLPVGLALR
                     ARFNPELNKAWFGGVMQIINNITMLSIVLTGAALIREREHGTVEHLLVMPVTPFEIMS
                     AKVLAMAAVVLLASAFALNVVVRGFMGVPIEGSLPLFFTGTALHLFATTSLGIFLATL
                     ARSMPQFGMMLLMVLLPLQMLSGGITPRESMPELVQLVMLAAPNTHFVLLSQGILYRG
                     AGLDVVWPQLMSLAVIGSTLFMFSLARFRKSLASMA"
BASE COUNT          609 a          966 c          929 g          493 t
ORIGIN      
        1 acctcacgct ccagcggcct gaacgcgacc cgcggcccca agatcaacgc gttcctgcag
       61 atcatccgca gctgccagga cgtgatgggt ctgttcattt tctcaggcgt gttcgaccgg
      121 catcccggac tgaagctggt ttgcgcagaa gcagacgcgg ggtgggtgcc ccactacatg
      181 taccgcatgg accacgccta cgaataccac cgctactgga tgaaggcgcc gccactcgaa
      241 cgtaaaccca gcgaatattt tcgcgatcac gtggccatga ccttccagga cgactacgtg
      301 gcgttccagt gcaaggatct ggtaaacgta cggcggctga tgtgggcgag cgatttcccc
      361 cacagcgatt ccacctggcc ccggtcgcaa tcggtgctcg cagagcagac ggctcacctg
      421 accgagcagg agagggactg gatctgccac gacaacgtcg ccggctggta cggtattacg
      481 gtatagaggt gcaccgagca ccaggaggca acgccatggc ctggaacttc gccgacgtct
      541 ttgaagccat cgccgagatc agtaacgaag agcatccggc ctgcatccat tctggcccgg
      601 gcggttcggc aggcatcacg atctcgtggc cggagctgat gcgccgcagc aaccggctgg
      661 cacgctatct ggccgcccag ggcctgcgcc cggacagcaa ggttgcgcac tacatgcgca
      721 actgtccggc gtacatcgag acgatgatcg caagcttcaa aggtcgctac gtccacgtga
      781 acgtcaatta ccgctaccgc gacaacgagc tgcactacat cctcgacaac gcggatgccg
      841 aagccgtggt gtacgacgcc gagttccgcg agcacgtggc agctctgcgt ccgcgactgc
      901 cacaggtacg gtcctggatc gaggtggctt cttattttga gcagccggca gatcgcgccg
      961 cacattttgc cgtctgctac gaagacgtcg tcggcaatgg caacgactca ccgctcgaca
     1021 tcgagcgcag cggcaacgac ctgctattca tctacaccgg aggcaccacc ggcatgccga
     1081 agggcgtgat gtgggagcac caagccatct ggcaggccgg cggctccggg tcgtcactgc
     1141 tgaaccctgg ccaggcggca ccagagtccc cgcgggaaca cgctcagcgc atcgcgaacg
     1201 cgcccttgcg ggccaggatg ctggccgcct gtccgctcat gcacggcacc gcgatgctga
     1261 cgagcatcaa cacgctgagt cagggcggct gcatcgtcac agtgcccgaa cacagtctcg
     1321 atccgcacgc catgtgggca acggtcgagc gctaccgcgt caactcgctc gccatcgtcg
     1381 gtgacgcgtt cgccaagccg ctgctgcggg cgctcgagga gagacctggc gcttacgaca
     1441 tctccagcgt gttcggcatc gtcagctcgg gcgtcatgtg gtccccggaa gtgaaacgcg
     1501 gcctgctgcg gcaccacccg aacatggttc tcatggattc cttcggcgcc tccgaggcca
     1561 tcggcttcgg cacctcccgg accaccggtg acgaggaagc cgccacagcc aagttcaaga
     1621 tcggcgagca ctgcaaggta ttcaccgaag atcatcgtcc cgtgcagccc ggcagtggtg
     1681 agccgggatt catcgcccgc tccggcccga ttccggtggg ctactacaag gacccggaga
     1741 aaacggcgaa gacgttcccc atgatcgacg gcgtgcgcta ctcagtccct ggcgactggt
     1801 gcctcgtgga gccagacggt acgttgaccc tgctcggccg gggctccgtg tgcatcaatt
     1861 ccggcggcga gaagatctat cccgaggaga tcgaggaagc gctcaagacc catcccgatg
     1921 tggaggacgc gttagtggtt ggagtgccgg acgagcgctg gggacaggcg gtcaccggcg
     1981 tgatcgtccc tatggatccg gcaacattcg acgaggctgc actgcgtgag cacgtcaagt
     2041 cacagctcgc accctacaag gcgcccaagc ggatcctgat ttcggaattt ccccttcggg
     2101 cgccgaacgg caaggccgac tataagagcg tcacggattt cgccagagcc gccttgggcg
     2161 acaccgcgga cgcaaagtaa gcctggagtc ccaaacggtg atcggtgtcc agccggtcgc
     2221 cggctccagt cctacgccat cgacgccagg gatttgcgga accgcgccag cgagaacatg
     2281 aacaaggttg acccgattac ggccaggctc atcagctgtg gccagaccac atccaggcct
     2341 gccccacgat agaggatgcc ctgagacaac agtacgaagt gggtgttggg tgcagccagc
     2401 atcacgagct ggaccagctc gggcatgctt tcgcgcggcg tgatgccgcc agagagcatc
     2461 tgcaggggca gcagcaccat cagcagcatc atgccgaact gcggcatcga gcgtgccagc
     2521 gtggccagga agatgcccag cgaggtggtg gcgaacaggt gcagggcagt gccggtgaag
     2581 aaaagcggca gcgatccttc gatgggcacg cccataaatc ctcgcaccac gacattgaga
     2641 gcaaaggccg aggcgagcag gaccaccgcc gccattgcca gcaccttggc ggacatgatc
     2701 tcgaacgggg tgaccggcat caccagcaga tgctccacgg taccgtgctc gcgctcccgg
     2761 atcagcgcgg cgccggtgag cacgatggac agcatggtga tattgttgat gatctgcatc
     2821 accccgccga accaggcctt gttcagttcc gggttgaaac gggcacgcag agccagtccc
     2881 accggcagac tcgtcgccgc gcgttgacgg ctcacgaact cgttgacctg atcctggacg
     2941 atggcctgaa tgtgaccgct gccggtgaag gcctgactca tgcgcgtcgc atccacg
//
LOCUS       DBNHQR010000388         3415 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_543397, whole genome shotgun sequence.
ACCESSION   DBNHQR010000388 DBNHQR010000000
VERSION     DBNHQR010000388.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_543397"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="AC16VR_08835"
     CDS             complement(<1..213)
                     /locus_tag="AC16VR_08835"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6011301.1"
                     /translation="MSDVEFGIGLRRLDSAAEDARLAESLGYEFVTTGEHVFFHGPVG
                     NGLISLAAAAGATQRIRLMSTITLVPL"
     gene            complement(210..1400)
                     /locus_tag="AC16VR_08840"
     CDS             complement(210..1400)
                     /locus_tag="AC16VR_08840"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6011302.1"
                     /translation="MADVESAEHYPLANLKVLDFSRVLAGPFAGRMLADLGADVVKVE
                     PPEGDITRLWGRVIGGVPGYYHQQNAGKRNICVDLRAPGARDLVFELVREADVLIENY
                     RADVMPRLGLAYETLKSVNPRLIMLSVSGFGHGGPESHRPAYAPIVHAEAGLLDRQAR
                     RGDIPFRDLPLSVADTNASLHGLVGVLAAVILRERTGLGQHLDIAMIDATVATDDQLH
                     YDLEDSEDTGPLRADTWQTGAGPILISADFRHLWRLLTGDLGLADPTHKEMDLETKIG
                     LRRQAVAEFMEGLETWESVEAAMASMNLAWGRVRNAATMREQPTVRARAAIVEVDDGA
                     GGRRPIPQSPYRFSAARSGVRGPAPGRGEHNLEVLGDWLGKTEADVEALVAGGVLKTG
                     EEES"
     gene            complement(1479..3113)
                     /locus_tag="AC16VR_08845"
     CDS             complement(1479..3113)
                     /locus_tag="AC16VR_08845"
                     /inference="COORDINATES: protein motif:HMM:NF025114.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6011303.1"
                     /translation="MNDEPDVLVVGAGFAGMYAVHRLGELGLTVKVLEAGGDVGGTWY
                     WNRYPGARCDVPSLEYSYGFSPELEQEWDWPEVFSSQEDILAYANHVADRFDLRRHMR
                     FDTRIVAVEYREDENVWRLTSEAGEVFEAPFCVMATGCLSVPNTPRLPGADSFAGLVL
                     HTGRWPHEAVDLAGKRVGIIGTGSSGVQAIPELARQSGHLVVFQRTPVYTVPANRKAM
                     RPDVQREFREHYRSIRGMQQRNAGGVSGYRPLRGGRAGPAVPGERALRTGILDLDPDQ
                     RRRIVAERGLETLLNFPDVYTDPEANEVANALYRDAVRAEVRDPETAEKLLPKDYGLG
                     CKRQVLDRDFFETFNRDDVTLVDLRETPMETLNPDGIRTSHGHYDLDVLIYATGFDAM
                     TGALTRANVRGRDGQALADKWRYGPVAYLGLQMRGFPNLFTVTGPGSPSVLCNMLVAI
                     EQHVNWIGECITWLRETNRTRIEPLAESETEWVAHVNEVAEGTMYTAPSCNSWYLGAN
                     IPGKPRIFMPYVGGYPRYRERCEREAASGYQGFESG"
     gene            3213..>3415
                     /locus_tag="AC16VR_08850"
     CDS             3213..>3415
                     /locus_tag="AC16VR_08850"
                     /inference="COORDINATES: protein motif:HMM:NF014816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6011304.1"
                     /translation="MPVGLHCRRRLEGEISVLILHHAPNSRAGRIVWLLEELGLDYEL
                     NRMDFHPKDLKSEAHRERHPLGRV"
BASE COUNT          562 a         1184 c         1101 g          568 t
ORIGIN      
        1 cagcggcacc agggtgatgg tgctcatgag ccggatgcgc tgcgtggcgc cagccgctgc
       61 cgccagcgag atgaggccgt tgccgaccgg gccgtggaaa aaaacgtgct cgcccgtcgt
      121 gacgaactcg taccccaggg attccgcgag gcgggcgtcc tcggccgcgc tgtcgagcct
      181 cctcagcccg atcccgaact ccacgtcgct catgattctt cctccccggt tttcaggaca
      241 ccgcccgcaa ccagcgcctc gacgtccgcc tctgtcttgc cgagccaatc tccgagcacc
      301 tcgagattgt gctctccccg acccggcgcc gggccgcgca cgccgcttct cgccgctgag
      361 aaccgatacg gggattgagg aatcggccgc cggccgccgg caccgtcgtc cacctccacg
      421 atggcggccc gggcgcggac agtgggctgc tcgcgcatgg tcgcggcgtt gcggacgcga
      481 ccccaggcca ggttcatgct ggccatggcg gcctccaccg attcccaggt ttccagccct
      541 tccatgaatt cggccaccgc ctgtcggcgc aggccgatct tggtttccag gtccatttcc
      601 ttgtgggtgg gatcagccag acccaggtcc cccgtgagca gccgccagag gtggcgaaag
      661 tccgcggaga tcaggatggg gcctgccccc gtctgccagg tgtccgcacg cagcgggccc
      721 gtatcctccg aatcctccag gtcgtagtgg agctgatcgt ccgtggcaac cgtggcgtcg
      781 atcatggcga tgtccaggtg ctggcccagt cccgttcgct cgcgaaggat gacggcggcc
      841 agcacgccga ccagcccgtg cagcgaggcg ttggtgtcgg caacggacag cggcagatcc
      901 cggaacggga tgtcgccgcg tcgggcctgc cgatcgagca gccctgcctc ggcgtgcacg
      961 atgggcgcgt aggccggccg atgagactcc ggcccgccgt gcccgaatcc ggataccgac
     1021 agcatgatga gacgcgggtt gacggacttg agagtctcgt aggcgaggcc gagacgcggc
     1081 atcacgtccg cgcggtaatt ctcgatgaga acgtccgcct cccgaaccag ctcgaagacg
     1141 agatcccggg ccccgggcgc gcgcaggtcg acgcagatgt tccgcttgcc cgcgttctgc
     1201 tgatggtagt agccgggaac gccgccgatg accctgcccc acaggcgcgt gatgtcgccc
     1261 tcgggcggct ccaccttgac gacgtcggcc ccgagatcgg ccagcatgcg cccggcgaac
     1321 ggccccgcaa gcacccggga gaagtccaga accttaaggt tcgcgagggg atagtgctcc
     1381 gcgctctcca catcggccat cgttgctcct cccctttccg accggggccg agcatagcat
     1441 cgaaagacgc ccgcgagacc gatcggcccg ggagccgttc agcccgactc gaacccctga
     1501 tagcccgacg ccgcctcgcg ctcgcagcgt tcccggtagc gcgggtaccc gccgacgtag
     1561 ggcatgaaga tgcgcggctt gcccggtatg tttgcgccga ggtaccagga attgcagctg
     1621 ggcgcggtgt acatggttcc ttcggccact tcgttgacgt gggcaaccca ctcggtttcc
     1681 gattcggcga gaggctcgat gcgcgtgcgg ttggtctcgc gcagccacgt gatgcactcg
     1741 ccgatccagt tcacgtgctg ctcgatggcc accagcatgt tgcagagcac cgaagggctg
     1801 cccggccccg taacggtgaa caggttcgga aacccgcgca tctgcagacc caggtacgca
     1861 accggaccgt atcgccactt gtctgccagg gcctggccat cgcggccgcg aacgttcgcc
     1921 ctggtcagcg cgcccgtcat ggcatcgaag ccggtggcgt agatcagcac gtcaagatcg
     1981 taatgaccgt ggctggtcct gatcccgtcg ggattgagcg tttccatggg ggtctcgcgc
     2041 aggtcgacca gcgtgacgtc gtcgcggttg aaggtctcga aaaagtcgcg gtccagcacc
     2101 tggcgcttgc atcccagccc atagtctttc ggcagcagct tctccgcggt ctccggatcc
     2161 cgcacctccg cccggaccgc gtcccggtaa agggcgttgg caacctcgtt ggcctccggg
     2221 tccgtgtaga cgtcggggaa gttgagcagc gtttccagac ctcgctcggc aacgatccga
     2281 cggcgttgat cggggtcgag atcgaggatg cccgtgcgca aggcgcgctc gccgggcacc
     2341 gcgggaccag cccgccctcc tctgagcggc cgatatccgg aaaccccacc ggcattgcgc
     2401 tgctgcatgc cgcggatgct gcgatagtgc tcacggaact cgcgctgcac gtcgggccgc
     2461 atcgccttgc ggttcgccgg gaccgtgtag acgggcgtgc gctgaaacac caccaggtgt
     2521 cccgactgac gagccagctc gggaatggcc tgaacgccgg aggatccggt gccgatgatg
     2581 cccacccgct tgcccgcgag atccacggct tcgtggggcc agcgtccggt atggagcacg
     2641 agcccggcga aggagtcggc acccggcagg cggggcgtgt tggggacgga caggcaaccg
     2701 gtggccatga cgcagaacgg cgcttcgaag acctcgcccg cctcgctggt caggcgccag
     2761 acgttctcat cctcccggta ctcgacggcg acgatccgcg tatcgaaccg catgtggcgc
     2821 cggagatcga accggtcggc cacgtggttc gcgtaggcga gaatgtcctc ctgtgaggaa
     2881 aatacctcgg gccagtccca ctcctgctcc agctccgggg aaaagccgta ggagtattcc
     2941 aggctcggaa cgtcgcagcg ggcgccgggg tagcggttcc agtaccacgt gccgccgacg
     3001 tctccacccg cctccagcac cttcaccgtg aggccgagct cgcctaagcg atgcacggcg
     3061 tacatccccg caaaacccgc acccaccacc agcacgtcag gttcgtcgtt catccgggcg
     3121 ccggattccc ctctttgaca tgacctgatt ctaagggcag ccgatgctca gcgtcaccgg
     3181 ccggccctgc agggaactcg cagcgatatt ccatgcctgt cgggttacac tgccgacgtc
     3241 gtctggaggg agagatttca gtgctgatac tgcaccacgc gcccaattca cgggccggac
     3301 gcatcgtctg gcttctcgag gagcttgggc tggattacga gctcaaccgc atggatttcc
     3361 atcccaagga cctcaagtcc gaggcccacc gcgaacgcca cccgctcggc cgcgt
//
LOCUS       DBNHQR010000389         2827 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_732948, whole genome shotgun sequence.
ACCESSION   DBNHQR010000389 DBNHQR010000000
VERSION     DBNHQR010000389.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_732948"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1165
                     /locus_tag="AC16VR_08855"
     CDS             <1..1165
                     /locus_tag="AC16VR_08855"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfotransferase family protein"
                     /protein_id="HIR6011305.1"
                     /translation="HPDLPQLHVSLGHAQKTIGDMNGAVASYQQAYGIRRDFGDAYWS
                     LANTKTYRFTPDEIDGMRQAEADPTCGTQDRIHLCFAAGKAFEDAGSYQESFEYYERG
                     NRLKQGEIRYDAGQIDRRVEAQMAVCTRELFARQEGHGDPAPDPIFVVGLPRAGSTLL
                     EQILASHSMVDGTMELPSLLAIAQRLRGRSSVRSDRYPRILGELKPEQLHQLGAHYLQ
                     ETLPYRQGAPRFVDKMPNNFFHVGLIRLQLPNARVIDARRHPMACCFSGFKQLFGEGQ
                     EFSYGLTEIGHYYRRYVELMKHWDEVLPGFVLRVQHEDVVDDLEGQVRRILEFCELPF
                     EDACVNFHRTERAIRTPSSEQVRQPIYRDGLDQWQRFEAWLDPLKAALGDALG"
     gene            1221..>2827
                     /locus_tag="AC16VR_08860"
     CDS             1221..>2827
                     /locus_tag="AC16VR_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011911733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HIR6011306.1"
                     /translation="MQSGDKAYAELDRPVFLTSASLLAAVIFLAALFPDTMDALFSDV
                     QGAIVAGASWYYVLVVAVILVSVLVFAFSSYGQIKLGPDHSEPDYSFISWFAMMFAAG
                     MGIGLMFFGVAEPVMHFLSPPKGDPGTIAAAREAMKLTFFHWGLHGWAIYAIVALILA
                     YFSFRHGLPLTLRSALYPLIGERIYGPIGTAVDVFAIISTTFGVATSLGLGVNQINAG
                     LNYLFGLPVTQEIRVGLIIVTTAIATVSVVLGLDRGIKRLSEINIVLAVMLMITVLAI
                     GPTTLLLQAFMQNTGDYLADLVGKTFNLYAYDPTDWLGGWTIFYWGWWISWAPFVGLF
                     IARISRGRTLREFVIGVLIGPVVFTFLWMTVFGNSAIDLIMNQGVTELGSAVQANESI
                     ALFRFLEQFPATGFLSALAIVMVVVFFVTSADSGALVLNMLSSNGSDQTPVGRRIFWM
                     SAIGVTAIVLLLVGGLSALQTAVIASALPFSVAVMAGIWGFSKALYVDHAKRVSHAAA
                     TVPPILARPEISWQHRLTQLLRHPQDA"
BASE COUNT          461 a          927 c          891 g          548 t
ORIGIN      
        1 tcatcccgac ctgccgcagc tgcacgtgtc gctcggtcac gcccagaaga cgatcggcga
       61 catgaacggc gccgtggcga gctaccagca ggcttacggg atccgccgcg acttcggcga
      121 tgcttactgg agcctggcca acacgaagac ctatcggttc acgcccgacg agatcgatgg
      181 gatgcggcag gcggaggcgg atccgacgtg cggcacgcag gaccgtattc acctgtgctt
      241 cgccgcgggc aaggccttcg aggacgcggg cagctatcag gagagcttcg agtactacga
      301 gcgcggcaac cgcctgaagc agggcgagat tcgctacgac gccggccaga tcgatcgacg
      361 ggtggaagcg cagatggcgg tctgcaccag agagctgttc gcccgccagg agggtcacgg
      421 agatcccgcg ccggacccga tcttcgtcgt cgggctgccg cgggcgggct cgacgctgct
      481 agagcagatc ctcgcctcgc actccatggt cgacgggacc atggagctgc cgtcgctgct
      541 cgccattgcc cagcgcctgc gaggccgcag ctcggtccgc tcggatcgct atccgcggat
      601 cctcggcgag ctgaaaccag agcagctcca ccagctcggc gctcactacc tccaggaaac
      661 cctgccctac cggcagggcg ccccgcgctt cgtcgacaag atgcccaaca acttttttca
      721 cgtcgggctg atccggctgc agctgccgaa tgccagggtc atcgacgcgc gccggcatcc
      781 catggcctgc tgcttcagcg gcttcaagca gctgttcggc gagggccagg aattcagcta
      841 cgggctgacg gagatcggcc actactaccg gcgctacgtc gagctgatga agcactggga
      901 cgaagtgctg cccggcttcg tgctcagggt gcagcatgag gacgtcgtgg acgacctgga
      961 gggccaggtc cggcgcatac tggaattctg cgagttacct ttcgaagatg cctgcgtgaa
     1021 cttccaccgc accgaacgtg ccatccgcac gccgagctcc gagcaggtac gccaaccgat
     1081 ttaccgcgat ggcctggatc agtggcaacg cttcgaggca tggctggatc cgctgaaggc
     1141 ggcgttgggc gatgcgcttg gttgaatttt cgagatggaa cgaagaaggt ggggcccctt
     1201 tggtcgcaag cgggacgtga atgcaatcgg gtgacaaagc ttacgccgag ctcgaccgac
     1261 ccgtattcct gacctccgcg tcgctgctcg ccgcggtgat ctttctggcg gcactttttc
     1321 ccgacaccat ggacgccctg ttcagcgatg tgcagggcgc catcgtggcc ggcgcgagct
     1381 ggtactacgt gctcgtggtt gccgtcattc tggtgagcgt gctggttttc gcgttctcga
     1441 gctatggcca gataaagctc gggcccgatc actcggagcc cgattattcc ttcatctcct
     1501 ggtttgccat gatgttcgcc gccggcatgg gcatcgggct gatgttcttc ggcgtcgccg
     1561 agccggtgat gcactttctc tcgcccccga aaggagaccc gggcaccatt gcagccgccc
     1621 gggaggcgat gaagctcacc ttcttccact ggggtctcca cggctgggcc atctacgcga
     1681 tcgtggcgct gatcctggcg tatttcagct tccggcacgg tctgccgctc acgctgcgct
     1741 cggccctgta cccgctgatc ggcgagcgca tctacgggcc catcgggacc gccgtcgacg
     1801 tcttcgccat catcagcacc accttcggcg tggccacctc cctcgggctc ggcgtaaacc
     1861 agatcaacgc ggggctgaac taccttttcg gtctgccggt gacccaggag atacgtgtcg
     1921 gtctgatcat cgtcaccacg gccattgcaa ccgtctccgt ggttctcggc ctggaccggg
     1981 gcatcaagcg gctgtccgag atcaacatcg tcctggccgt catgctgatg atcaccgtgc
     2041 tggccatcgg cccgaccacc ctgctcctcc aggcgttcat gcagaacacc ggcgactacc
     2101 tggccgacct ggtgggaaag acgttcaacc tctacgccta cgaccccacg gactggctgg
     2161 ggggatggac catcttctac tggggctggt ggatcagctg ggcgcccttc gtggggctct
     2221 tcatcgcgcg gatctcccgg ggcaggaccc tgcgggagtt cgtgatcggc gtgctgatcg
     2281 gtcccgtcgt cttcaccttc ctgtggatga ccgtgttcgg caacagcgcc atcgatctga
     2341 tcatgaacca gggcgtgacg gagctcggct cggccgtgca ggccaacgag tccatcgccc
     2401 tgtttcgctt cttggagcag tttcctgcca ccggatttct gtctgccctg gccatcgtga
     2461 tggtcgtggt ctttttcgtg acctcggcgg actcgggggc cctggttctg aacatgctgt
     2521 catccaacgg ctcggatcag acaccggtgg gccggcggat cttctggatg agcgccatag
     2581 gggtgacggc catcgtgctg ctgctggtgg gcgggttgtc cgcgctgcag accgccgtca
     2641 tcgcaagcgc cctgcccttc tccgtggccg tcatggccgg aatctggggg ttcagcaagg
     2701 cgctctacgt cgatcacgcc aaacgcgtgt cccatgcggc agcgaccgtg ccgcccattc
     2761 tcgccaggcc cgagatctca tggcagcatc gactgacaca gctgctgcgc cacccccagg
     2821 acgccga
//
LOCUS       DBNHQR010000390         4802 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1050230, whole genome shotgun sequence.
ACCESSION   DBNHQR010000390 DBNHQR010000000
VERSION     DBNHQR010000390.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4802)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1050230"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..144)
                     /locus_tag="AC16VR_08865"
     tRNA            complement(68..144)
                     /locus_tag="AC16VR_08865"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(108..110),aa:Asp,seq:gtc)
     gene            complement(162..237)
                     /locus_tag="AC16VR_08870"
     tRNA            complement(162..237)
                     /locus_tag="AC16VR_08870"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(202..204),aa:Val,seq:tac)
     gene            337..759
                     /locus_tag="AC16VR_08875"
     CDS             337..759
                     /locus_tag="AC16VR_08875"
                     /inference="COORDINATES: protein motif:HMM:NF019500.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HIR6011307.1"
                     /translation="MDYLLATAVSVVLVALCISIHYEMLNLMARVVHRPERHRWIIMV
                     MILGLLFAHVVEIWLFGFGYLLAQEQLALGHITGTGDGLFDYIYYSAMVYTTVGFGDQ
                     VPGGAIRMISSTEALAGLSLITWSASFTFLQMQRLWRD"
     gene            878..2437
                     /locus_tag="AC16VR_08880"
     CDS             878..2437
                     /locus_tag="AC16VR_08880"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF017085.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050355 - inorganic triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH and CHAD domain-containing protein"
                     /protein_id="HIR6011308.1"
                     /translation="MNELEAKYLVAAGRKPRRVLEHVLQTLSWAGYRARLRSERLVVD
                     TYFDTPDWALQRAGWAYRLREDEQKKTRTLAVKAIKTGHYGVFRREEFEQSLDAPLNR
                     PGDPPAGQVADQLWVIVTEEASLKPLFHLRNQRTKYDITSDAYPGGTVELSLDRVTVD
                     EAPGCGYTELELELLEGPEDILNDIVEMLNQQSDIFLARVGKYQRGLLASGRPPSLPS
                     AEPTPSPDKECRWLDLGLYNLRCELDDIRRYEPHAWESIHPEGVHQMRVATRRARAAL
                     RAFGDVLPPKAAKRLTRDIRWLTSSLGPLRDLDVQIAHIDAERERLDARDASAQALYR
                     KHLEKKRRRAHRRLVEALASDEYRKLIADFERLLEASASYHGPNRLMTIREAAHTQMK
                     PQLDRLLARGRNARKRPASRRLHRLRIALKRLRYQLEYLEGSYGKPMGNLTRAMEDVQ
                     DSLGNHQDAMVAVAQLERFRRKGGLKSGSRKSVKRLIAAEQENAARYRKRFRKDWRKF
                     EEAAAKMVKRL"
     gene            complement(2442..3257)
                     /locus_tag="AC16VR_08885"
     CDS             complement(2442..3257)
                     /locus_tag="AC16VR_08885"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR6011309.1"
                     /translation="MPRRPARWLLSGWCVTALLGLAACEPGHTPFPDGEIVDLSHTYD
                     ETTVFWPTADGFQLEVESAGYTDKGYYYAANNFSTSEHGGTHLDAPIHFAEGAPATDE
                     IPLERLVGPGVVIDVSAACTDDADYQIDVADVTRWERANGQTVDGMIVLFRTGFAERW
                     PDVARYLGTAERGAEAVAKLHFPGLHPELAAWLVAERDVKAVGLDTASIDYGQSTLYE
                     THQILFKAGIPAFENVANVSRLPASGFSVVALPMKIGGGSGGPLRIIAIVPPA"
     gene            complement(3258..3998)
                     /locus_tag="AC16VR_08890"
     CDS             complement(3258..3998)
                     /locus_tag="AC16VR_08890"
                     /inference="COORDINATES: protein motif:HMM:NF018961.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin domain-containing protein"
                     /protein_id="HIR6011310.1"
                     /translation="MAEPFRPAPPRTGFGQALRSKRASTMEAVEDLLAHCESLLDVTV
                     QAVDGIAEAQGVDLETRFASDRKDLYRRYLAHCLDDKILTEDENADLQHLLNLLHLNP
                     DDVVPVHDEMAREVYGKAIQEVLADLEVDADEEAFLRRLRGDLKLSDDVASDLLERGR
                     RDAHDVALREASTPDHDFLVYRAPAGEFTGRSDVSFEAAVTDALSKAVIAIPMLHWFE
                     VSNISGYVGDGKPRGWHVTVRGGIEPEK"
     gene            4152..>4802
                     /locus_tag="AC16VR_08895"
     CDS             4152..>4802
                     /locus_tag="AC16VR_08895"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HIR6011311.1"
                     /translation="MALELPFRSAIELAHLIQTREVSSVELTRTYIERIERYDGAVNA
                     VPVRMFDQALADAAEADAVLARGEDVGPLHGVPMTIKESYVLKDTPATWGLEAFKDNV
                     ATRDGLAVSRFRQAGAHFLGKTNVPVDLADFQSYNPIYGTTGNPWNTERIPGGSSGGS
                     SAALAAGFGALEAGSDIGGSIRNPAHYCGVYGHKPTWGIVPMSGHELMEGVPDADLS"
BASE COUNT          845 a         1646 c         1550 g          761 t
ORIGIN      
        1 taacccgctg aaagtacggg ccctttcaga agagggtcaa cagttagcag gagtctaaat
       61 gacttgatgg cggagcggac gggactcgaa cccgcgaccc ccggcgtgac aggccggtat
      121 tctaaccaac tgaactaccg ctccgatgtt cccttcacta gtggtgggtg ttgcaggggt
      181 cgaacctgcg acctacggct tgtaaggccg tcgctctccc aactgagcta aacaccctgg
      241 ggaatcaagg gggcgtatct taccccctga cctatggctg tcaatcggat ccgccggcac
      301 gtgggaggcc ttgggcagtc gcgctacaat ccgcccatgg actacctcct ggccaccgcg
      361 gtttccgtcg tcctcgtcgc gttgtgcatc tccatccact acgagatgct gaacctcatg
      421 gccagggtcg tgcaccggcc cgaacgccac cgctggatca tcatggtcat gatcctgggg
      481 ctgctgttcg cccacgtggt ggagatctgg ctgttcggct tcggctacct gttggcccag
      541 gaacagctgg cgctcgggca catcaccggc accggcgacg ggctgttcga ctacatctat
      601 tactcggcca tggtctacac caccgtgggc tttggcgacc aggtgccggg aggggccatc
      661 cgcatgatct ccagcaccga ggccctggcc gggctttcgc tgatcacctg gtcggcgtcc
      721 ttcaccttcc tgcagatgca gaggctctgg cgcgactgag caccgggcga tcgccaccga
      781 tcggcgccgc cggcgatacc gtcgatcgga tcatcgatga cagggcgcgc cgattcgggt
      841 aataatctgt caacttcttt gatttatttc cccgcccgtg aacgagctgg aagccaaata
      901 tctcgtcgct gcgggcagaa agcccaggcg cgttctggag catgtgctgc agacgctgag
      961 ctgggccggt taccgggcca gactgcgctc agagcgcctg gtcgtcgaca cctacttcga
     1021 cacgccggac tgggccctgc agcgggccgg atgggcctac cgcctgcgcg aggacgagca
     1081 gaagaagacg cgcaccctgg ccgtgaaggc catcaagacc gggcactacg gcgtgttcag
     1141 gcgcgaagag ttcgagcagt ctctcgatgc ccccttgaac cgccccggag acccgcccgc
     1201 cggccaggtg gccgaccagc tatgggtgat cgtcaccgag gaagcttccc tgaagcctct
     1261 gttccacctg cgcaaccagc gcacgaaata cgacatcacc agcgacgcct atccgggcgg
     1321 cacggtggag ctgtccctgg accgggtcac cgtggacgag gccccgggct gcgggtacac
     1381 cgagctggag ctggagctac ttgaagggcc cgaggacatc ctgaacgaca tcgtggagat
     1441 gctgaaccag cagtcggaca tcttcctggc ccgggtgggc aagtaccagc ggggcctgct
     1501 cgccagcgga cgcccgccct cactgccgag cgcagagccg acgcccagcc cggacaagga
     1561 gtgccgctgg ctggacctcg gcctgtataa cctgcgctgt gagctggacg acatccggcg
     1621 ctacgagccc cacgcctggg aatccatcca cccggagggc gtccaccaga tgcgggtggc
     1681 cactcgccgg gcccgggccg ccctgcgcgc cttcggcgac gtcctgccgc ccaaggccgc
     1741 caagcgcctc acccgggaca tccgctggct cacctccagc ctgggcccgc tgcgcgatct
     1801 ggacgtgcag atcgcccaca tcgacgcgga gagggaacgg ctcgacgcca gagacgcatc
     1861 ggcccaggcg ctctaccgga agcacctgga aaagaagcgc cgacgcgccc accggcgcct
     1921 ggtggaagcg ctggccagcg acgagtatcg caagctcatc gcagacttcg aacggcttct
     1981 ggaggcttcc gccagctacc acggccccaa ccggctgatg accatccgcg aggccgccca
     2041 cacgcagatg aagccgcagc tggaccggct gctggcccgg ggacgcaacg cccgcaagcg
     2101 ccctgcctcg cggcggctgc accggctgcg gatcgccctg aagcggctgc gataccagct
     2161 ggagtacctg gagggttcgt acggcaaacc catgggcaac ctcaccaggg ccatggagga
     2221 cgtccaggat tctctgggca accatcagga cgccatggtg gccgtcgctc agctcgagcg
     2281 atttcgcagg aagggagggt tgaagtccgg ctcgcgcaaa tccgtgaagc ggctgatcgc
     2341 cgcagagcag gaaaacgccg cgcgttaccg caagcgcttc cgcaaggact ggcgcaaatt
     2401 cgaggaagcc gcggcgaaga tggtcaagcg gctgtagccc ttcaggcggg tgggacgatg
     2461 gcgatgatcc gcaacgggcc gccgctgccg ccaccgatct tcatgggcaa cgccaccacc
     2521 gagaagccgc tggccggaag gcgtgagacg ttggccacgt tctcgaacgc cgggatgccc
     2581 gccttgaaca ggatctggtg ggtctcgtaa agggtcgact gaccgtagtc gatgctggcc
     2641 gtgtccagac ccaccgcctt cacgtcgcgc tcggccacga gccacgccgc cagctccgga
     2701 tgcagcccgg gaaaatgcag cttggccacc gcctccgcgc cgcgctcggc ggtgccgaga
     2761 tagcgcgcga catccggcca gcgctcggcg aacccggtgc ggaacagcac gatcatgccg
     2821 tccaccgtct ggccgttagc acgctcccat cgggtgacgt ccgccacgtc gatctggtag
     2881 tcggcgtcgt ccgtgcaggc agcgctcacg tcgatcacta cccccggccc caccagcctc
     2941 tccagcggga tctcgtcggt ggcgggcgcg ccctccgcga agtggatggg cgcgtccagg
     3001 tgcgtcccgc cgtgctcgga ggtggagaag ttgttggccg cgtagtagta gcccttatcc
     3061 gtgtaaccgg ccgactccac ctccagctga aagccgtcgg ccgtgggcca gaacacggtg
     3121 gtctcgtcgt aggtgtgaga gagatcgacg atctcgccgt ccggaaacgg cgtatgccca
     3181 ggctcgcagg cggccagacc cagcagggcg gtgacgcacc acccggacag gagccatcgg
     3241 gcgggacggc gcggcatcta cttctccggc tcgatcccgc cccgcacggt cacgtgccag
     3301 cccctcggtt tgccgtcgcc tacatagccg gagatgttgg agacctcgaa ccagtgcagc
     3361 atggggattg cgatcacggc cttggagagc gcgtcggtca cggccgcctc gaaagacacg
     3421 tccgaccgcc cggtgaactc gccggcgggg gcgcggtaga cgaggaagtc gtgatccggc
     3481 gtggatgcct cgcgcagcgc cacgtcgtgg gcgtcccggc ggccgcgctc gagcaggtcg
     3541 gaggccacgt cgtcggagag cttcagatcg ccgcgcaggc gtcgcagaaa ggcttcctcg
     3601 tccgcatcca cctcaagatc ggccagcact tcctggatcg ctttgccgta gacctcccgg
     3661 gccatctcgt cgtgcacggg aaccacgtcg tccggattca gatgcagcag attgagcagg
     3721 tgctgaagat cggcgttctc gtcctctgtg aggatcttgt cgtcaagaca gtgggcaaga
     3781 tagcggcggt acaggtcctt gcgatcgctc gcaaagcgcg tctccagatc caccccctgg
     3841 gcttcggcga tcccgtccac ggcctgcacg gtcacgtcca gaagcgactc acaatgcgcc
     3901 agcaggtcct cgacggcttc catggtactc gcgcgcttcg agcgcagggc ctgcccgaac
     3961 ccggtcctcg gtggggctgg tcgaaacggt tctgccacgg gtctactcct ctcctcgcgt
     4021 gaaggggggc attgtaggcc accttgagcc gattgtggct accaggccgc aagctcctcg
     4081 acggggacga attgcctccc aaccggctag gggtcattct tgagtcccga gaccaacgat
     4141 cagggggaaa gatggcgctc gaactgccct tccgctcggc catcgagctg gcacacctca
     4201 ttcagacccg cgaggtcagc tccgttgagc tgacgcgcac ctacatcgag cgcatcgagc
     4261 gttacgacgg cgccgtcaac gccgtgcccg tgaggatgtt cgaccaggcc ctggccgacg
     4321 ccgccgaggc cgacgccgtg ctggcccgcg gcgaggacgt gggcccgctg cacggcgtgc
     4381 ccatgaccat caaggagtcc tacgtcctga aggacacgcc ggccacctgg ggcctggagg
     4441 ctttcaagga caacgtggcg accagagacg gcctggccgt gagccggttc cgccaggccg
     4501 gcgcccactt cctgggcaag acgaacgtcc ccgtggatct ggccgacttc cagagctaca
     4561 accccatcta cggcaccacg ggcaacccct ggaacaccga gcgcatcccc ggcggctcct
     4621 cgggaggcag ctcggcggcc ctggcggccg ggttcggcgc cctggaagcc ggctcggaca
     4681 tcggcgggtc catccgcaac ccggcccact actgcggcgt gtacggccac aagcccacct
     4741 ggggcatcgt gcccatgtcc ggccacgagc tgatggaggg cgtgcccgat gccgacctct
     4801 cc
//
LOCUS       DBNHQR010000391         2210 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_607001, whole genome shotgun sequence.
ACCESSION   DBNHQR010000391 DBNHQR010000000
VERSION     DBNHQR010000391.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_607001"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="AC16VR_08900"
     CDS             complement(<1..257)
                     /locus_tag="AC16VR_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011312.1"
                     /translation="MDWERYKQLCDQPNVFSRWMLNQTIELIGESPVADTLAGVLGSF
                     PLEKPDDHRGGPATDMFVLELESGVRRAVVELVLLARDAGAA"
     gene            294..1391
                     /locus_tag="AC16VR_08905"
     CDS             294..1391
                     /locus_tag="AC16VR_08905"
                     /inference="COORDINATES: protein motif:HMM:NF053593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv3169 family protein"
                     /protein_id="HIR6011313.1"
                     /translation="MLTKADDYPIHQTPEPIAYSGTDRNFYDRYFFNGYQREGELFFA
                     AALGVYPHLNVMDAAFSILVGGRQYNVQASKVLHMERLNTQVGPIEVEVIEPLNVLRV
                     SCADPANGVAADVCFRARVPAVEEPRFTRRQGSLTFMDYTRLTQNGSYEGWLEVDGQR
                     YELTHDETWGTRDRSWGIRSVGARDPQPNPYAGEPQFYWLWAPLNFPSTNNYYHLNAD
                     REGRPWNTMGRLADPERSEEMAAAASSIRHRSGTRLAESATLEYVRENGEEATIELQP
                     TQTFYMRGLGYMHPEWGHGLFHGELATNYEVYETAEIADADPGYFHVQAICDAQLTDA
                     RGSERGKGVLEQLVIGAYEPYGFRELFDVAP"
     gene            1525..>2210
                     /locus_tag="AC16VR_08910"
     CDS             1525..>2210
                     /locus_tag="AC16VR_08910"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013482.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR6011314.1"
                     /translation="MSTAKEPQDPAGGVSRPDEQAVPSALPRAFGASVKRKEDRRLIT
                     GRGNYTDDLLLPDQAFAAFVRSPHAHAAINSIDAEAAREMPGVVAVLTGEEFAASGLG
                     PLMCGWMIHSRDGEPMKVGEHPALASDKVRYVGDAVALVVADSLQAARDAAECVYVDY
                     DDLPAVADVVSAGSVDAPELHSGIAGNEAFDWEIGDADAVAAAFARAAHITRLRLINN
                     RLVPNAMEPRS"
BASE COUNT          393 a          748 c          721 g          348 t
ORIGIN      
        1 gcggcgcccg cgtcccgagc caggaggacc agctccacca cggcacgccg gacacctgat
       61 tcgagttcca gaacgaacat gtccgtcgcg ggtccgccgc ggtgatcgtc cggcttctcc
      121 aggggaaacg agcccaacac ccctgccagg gtatcggcga ccggactctc tccgatcagc
      181 tcgatggtct ggttgagcat ccatcgggaa aagacgttgg gctgatcgca cagctgcttg
      241 tagcgctccc agtccacgag taacctgatt ttccgccccc ctggaagagg tcgatgttaa
      301 caaaagcgga cgactatccc atccaccaga ccccggagcc catcgcttat tccgggacgg
      361 atcgcaactt ctacgatcgc tatttcttca acgggtacca gcgcgagggt gagctgttct
      421 tcgctgcggc gcttggcgtc tatccgcacc tgaacgtcat ggatgccgcc ttcagcattc
      481 tggtcggcgg caggcaatac aacgtgcagg cgtccaaagt gctccacatg gagcggctga
      541 acacccaggt gggtcccatc gaggtcgagg tcatcgagcc gctcaacgtc ctgagggtga
      601 gctgcgcgga tcccgccaac ggcgtggcgg cggacgtctg ttttcgtgcc cgggtccccg
      661 ccgtggaaga accgcgcttc acccgccgtc agggtagcct caccttcatg gactacaccc
      721 ggctcaccca gaacggcagc tatgagggct ggctcgaggt cgacggccag cgttacgagc
      781 tcacccacga cgagacctgg ggaacccggg accggtcctg gggcatccgc tcggtgggcg
      841 cacgcgaccc ccagcccaac ccgtacgcgg gtgagcccca gttctactgg ctgtgggcgc
      901 ccctgaactt cccgagcacc aacaactatt accacctcaa cgcggatcgc gaaggtcgac
      961 cctggaacac catgggccgc ctggccgatc cggagcgcag cgaggagatg gcggccgcag
     1021 cgagcagcat ccggcaccgg tccggcaccc ggctggccga gagcgccacc ctcgagtacg
     1081 tgcgggaaaa cggcgaggag gcgaccattg agctgcagcc gacccagacc ttctacatgc
     1141 gcgggctggg ctacatgcat ccggagtggg gccacgggct gttccacggc gagctggcga
     1201 cgaattacga ggtctacgag accgccgaga tagccgatgc agatcccggc tattttcacg
     1261 tccaggcaat ctgcgatgcg cagctcacgg atgcccgcgg ctcggagcgc ggcaaaggcg
     1321 tgctggagca actcgtcatc ggcgcctacg aaccctacgg cttccgcgag ctcttcgacg
     1381 tggcgccatg acctgcggct gccccctgcc aagcgccctg gccctttacc cacgattggc
     1441 acgatgcggt aagcttgccg gcggcccggt ccgtgggcgg cgtcgccgcg gcataccggg
     1501 atttgcgggt ggggagagcc aaagatgagc accgcgaagg agccgcaaga cccggcgggc
     1561 ggggtatcgc gacccgatga gcaagccgtt ccgagcgccc ttccgcgggc gttcggcgcg
     1621 tcggtgaagc gcaaggaaga ccggcgcctg atcaccgggc gcgggaacta caccgacgac
     1681 ctgctcctgc ccgatcaggc cttcgccgcc ttcgtgcgat ccccccacgc tcacgctgct
     1741 atcaattcca tcgacgcgga agcggccagg gagatgcccg gcgtggtcgc cgtgctgacg
     1801 ggcgaggaat tcgcggccag cggtctcggc ccgctgatgt gcggctggat gatccactcc
     1861 cgcgacggcg agcccatgaa ggtgggcgag cacccggcgc tggcgtcgga caaggtccgc
     1921 tacgtcggcg acgcggtcgc cctggtggtt gccgactcgc ttcaagccgc acgggacgcc
     1981 gctgagtgcg tgtacgtcga ctacgacgac ctgccggcgg tcgccgacgt ggtttcggca
     2041 gggtccgtcg atgcgccgga gcttcattcg gggattgcag gcaacgaagc gttcgactgg
     2101 gaaataggcg atgccgacgc ggtagcagcc gctttcgcca gggctgccca catcacccgg
     2161 ctgcggctga taaacaaccg cctggtgccg aacgccatgg agccgcgctc
//
LOCUS       DBNHQR010000392         2525 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_670544, whole genome shotgun sequence.
ACCESSION   DBNHQR010000392 DBNHQR010000000
VERSION     DBNHQR010000392.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_670544"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="AC16VR_08915"
     CDS             complement(<1..869)
                     /locus_tag="AC16VR_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007942367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIR6011315.1"
                     /translation="MKKLMLGLAVLCVSAPAFADDLSQGNTAWILTSTALVLFMTIPG
                     LSLFYCGLVRTKNVLSVLMQCFVITCFVSILWLLVGYSLAFDADGGNAIIGGLGKVLF
                     AGVSESSMSGDIPETAFALFQLTFAIITPALIVGAFAERMRFSAMLMFSGAWLLLVYA
                     PICHWVWGGGWLGDLGLLDFAGGTVVHITAGVAAVVAALTLGSRRGFPQQAMPPHNMT
                     LTIAGAGMLWVGWFGFNGGSALAANGDAAMAMLVTHLSASAGAFTWMCAEWVRYGKPS
                     ALGAVTGMVAGRGT"
     gene            1116..2096
                     /locus_tag="AC16VR_08920"
     CDS             1116..2096
                     /locus_tag="AC16VR_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6011316.1"
                     /translation="MQNTEAAVSVEQEKVTTVTDWLSKRIIGQETLLQRLMTALLCGG
                     HLLVEGAPGLAKTRAIKELASAVQGDFHRIQFTPDLLPSDITGTEVYRPEDGSFKFQH
                     GPVFHNLLLADEINRAPAKVQSALLEAMAEHQVSVGRQTFPLPELFLVMATQNPIEQE
                     GTYPLPEAQLDRFLMHVTVSYPSESAETSILHMVRKETGHRDEAATPPFRLTEADIFD
                     ARRAILGLHMAEPVERYIVALIMASREARGELGQWLEFGASPRGTIALDQCARALAWL
                     ERRDYVTPDDVRAIAHDVLRHRLILTFDADAQGVTTDQVIDQLLAEVPVP"
     gene            2159..>2525
                     /locus_tag="AC16VR_08925"
     CDS             2159..>2525
                     /locus_tag="AC16VR_08925"
                     /inference="COORDINATES: protein motif:HMM:NF013998.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR6011317.1"
                     /translation="MDLGDLLSIRYRPAHSKTRPARRVSGLAGNRLSKLRGRGIDFSE
                     VRAYQPGDDVRTIDWRVTARKNKPHTKVFREERERLTLVFVDQSQGMFFGSRARLKSV
                     AAAEVAATAAWQALAHNDRV"
BASE COUNT          489 a          853 c          786 g          397 t
ORIGIN      
        1 gtcccgcgcc cggcgaccat acccgtcacg gcgcccagcg cgctgggctt gccgtatctg
       61 acccactcgg cgcacatcca ggtgaacgcg ccggcggacg cggacagatg ggtgaccagc
      121 atggccatcg cagcgtcgcc gttggctgcc agggcagacc cgccgttgaa tccgaaccac
      181 cccacccaga gcatcccggc ccccgcaatg gtgagcgtca tgttgtgcgg cggcatggcc
      241 tgctgcggga aaccgcggcg ggacccgagc gtgagcgctg cgaccaccgc ggcaacgccg
      301 gcggtgatgt ggaccacggt gccgccggca aaatccagga gcccgaggtc gccgagccag
      361 ccgccgcccc agacccagtg acatatcgga gcgtatacga gcagcagcca agcaccgctg
      421 aacatcagca tcgcagagaa gcgcatccgc tcggcgaagg cgcccacgat gagcgcgggg
      481 gtgatgattg caaaggtaag ctggaagagc gcgaaggccg tctccgggat gtctcccgac
      541 atggaactct cagacacccc ggcgaacagg accttgccca gcccgccgat gatggcgttg
      601 ccgccgtcgg cgtcgaacgc caggctatat cccaccagca accagaggat ggacacgaag
      661 cacgtgatga cgaagcactg catgagcacg ctgagcacgt tcttggtccg gacgagcccg
      721 cagtaaaaga gggaaagacc aggaatggtc atgaacagaa ccagcgccgt cgacgtgagg
      781 atccaggccg tgttgccctg gctcaggtcg tcagcgaacg cgggcgccga cacacacagc
      841 acggccaacc ccaacatcag ttttttcatc gatagtcccc tcttatgtcg ccacccgccc
      901 ggctttctgt gtctccggtt cccggatgcc cgcacaccgc ttgatttcat caacgaattt
      961 acttcgattt cgccaggggt gccagaggcg atccgcgtgc tgctcgattg acgcggaccg
     1021 gaacgccggc gtcgaggatg tatttcgttg ccggaaggct acaatacccg gcccggacgc
     1081 cccggcgggc ttgcccgggc gcgcatgagg acgatatgca gaacacggaa gcagcagttt
     1141 cggtcgaaca ggagaaggtc actacggtga ccgactggct atctaagcgc atcatcgggc
     1201 aggagaccct gctccagcgt cttatgaccg cgctcctttg cggcggccat ctgctggtag
     1261 aaggcgcgcc ggggctggcc aagacccgcg ccatcaagga acttgcaagc gccgttcagg
     1321 gcgattttca tcgcatccag ttcacgcccg atctgctgcc cagcgacatc accggaaccg
     1381 aggtgtaccg cccggaggac ggctcgttca agtttcagca cggtcccgtt ttccacaatc
     1441 tgctgcttgc cgacgagatc aaccgtgccc cggcgaaggt tcaatcggcg ttgctggagg
     1501 cgatggccga gcatcaggtc agcgttgggc gtcagacgtt ccccctgcct gaactttttc
     1561 tcgtcatggc gacccagaac cccatcgagc aggagggcac ctacccgctg cccgaggccc
     1621 agctcgaccg cttcctgatg cacgtgacgg tcagctaccc ctcggaatcc gcggagacgt
     1681 ccattctcca catggtccga aaggaaaccg gacacaggga cgaggcggca acaccgccct
     1741 tccggctgac cgaagccgac atcttcgacg cgcgccgcgc gatactcggt ctgcacatgg
     1801 ccgaacccgt ggagcgctac atcgttgcgc tgatcatggc gtcgcgggaa gcgcgcggag
     1861 agctgggcca atggctggaa ttcggcgcaa gcccacgggg caccatcgcg ctggaccagt
     1921 gtgcccgggc actggcctgg ctggaacgca gagactacgt cacacccgac gacgtgcgcg
     1981 cgatcgctca cgacgttctg cggcatcggt tgatcctcac cttcgacgcg gacgcccagg
     2041 gcgtcacgac ggaccaggtc atcgaccagt tgctcgccga ggtgccggtt ccctgagctc
     2101 gccgactgct agcacgatcc tcggctcggg tcgggaagaa atcctgaccg gcgcctacgt
     2161 ggacctcggc gaccttctgt ccatacgcta ccggccggcc cactcgaaaa cgcgaccggc
     2221 gcggcgggtc agcgggctgg cgggcaaccg cctgtccaag ctgcggggcc ggggcatcga
     2281 cttctccgag gtgcgtgcct accagcctgg cgacgatgta cgcaccatcg actggcgggt
     2341 cactgcgcgc aaaaacaagc ctcacaccaa ggtatttcga gaggaacgcg aaaggctcac
     2401 cctggttttc gtcgatcaga gtcagggcat gttcttcggc agccgcgccc ggctcaaatc
     2461 cgtcgctgcc gccgaggttg ccgccaccgc cgcctggcag gcgctcgctc acaacgatcg
     2521 cgtcg
//
LOCUS       DBNHQR010000393         1503 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1429829, whole genome shotgun sequence.
ACCESSION   DBNHQR010000393 DBNHQR010000000
VERSION     DBNHQR010000393.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1429829"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..533)
                     /locus_tag="AC16VR_08930"
     CDS             complement(114..533)
                     /locus_tag="AC16VR_08930"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6011318.1"
                     /translation="MSDQYVADRIALQDVMLKYAAGVDNRDFDLYRSCFADDVEVLGF
                     GDETVNGGDAWLAYVKNALERFGPTQHMLGPQLATVDGDDAHCRTDVQALHYLKGEES
                     TTLTLWATYETDMRRIDGTWKIVRHRLVSRGTRIQSD"
     gene            699..>1503
                     /locus_tag="AC16VR_08935"
     CDS             699..>1503
                     /locus_tag="AC16VR_08935"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6011319.1"
                     /translation="MGSLQGKIAVVTGASRGIGKGIALALGEQGCTVYVTGRTRGDGE
                     RTIDTTARLVTEAGGEGRAIQCDHGDDEQIAALFQQVKEEAGHIDILVNNVYKIPDPP
                     AWGGGFWDHPIQVWDDQVGIGLRAHYVASWHAAPLIFAAGAGGAILNVSSPGGQSYHF
                     SSSYGAGKAGLDRLTADMAIELEPKGVAAVVLYPGSVATEFIQDHAEAQGMDLSESQT
                     PLYVGRCAVAMLAADDLMSRSGSIQWVEDLGEEFDILDEHGKRPPGYAQR"
BASE COUNT          256 a          507 c          473 g          267 t
ORIGIN      
        1 gtccggcagg tccgggaacc cgtcgggcgg cgagttgcgg ccgacctcgt actccatcag
       61 ctgcctgagc gtgcggattt cctgctcgtt cataggatcg attcctcaac cgttcagtct
      121 gattggatcc gtgtcccccg cgacaccagc ctgtgacgga cgattttcca cgtgccatcg
      181 attcggcgca tgtccgtttc gtaagtcgcc cacagcgtca gcgtggtgct ctcctctccc
      241 ttcagataat gcagcgcctg cacgtcggtc cggcagtgcg catcgtcgcc gtccacggtg
      301 gccagctgcg ggcccagcat gtgctgagtg ggcccgaaac gttccagggc gttcttgacg
      361 taagccagcc acgcatcgcc gccgttgacg gtctcgtcgc cgaatcccag cacttccaca
      421 tcgtcggcga agcacgaccg gtaaaggtcg aaatcgcggt tatccacccc ggcggcgtat
      481 ttgagcatga cgtcctgcag ggcgatgcgg tcagccacgt attgatcact catccctctc
      541 tccctcgagc caatgtcctt caaatctagc ccataaagga agagcgcacc agccgggcgg
      601 gccgccggcg agcactggca atctcgccgc gccccggtgc ctgggtgggc aggcttctgg
      661 tatcctgcgc gccccgctca ctgcattggg aaacagatat gggttcgtta caaggaaaaa
      721 tcgccgtggt caccggggcc agccggggca tcggcaaggg tatcgccctt gctctgggcg
      781 agcagggctg caccgtctac gtgaccggcc gcacacgcgg cgacggtgag cggaccatcg
      841 acaccaccgc ccggctggtg accgaggcag ggggcgaggg ccgagccatt cagtgcgacc
      901 atggggacga cgagcagatc gccgccctgt tccagcaggt caaggaagaa gccgggcaca
      961 tcgacattct cgtcaacaac gtctacaaga tccccgatcc gcccgcctgg ggcggcggtt
     1021 tctgggatca cccgatacag gtctgggacg atcaggtggg cattggcctg cgcgcccact
     1081 acgtggccag ctggcatgcc gcccctctga ttttcgcggc gggtgctggc ggcgccattc
     1141 tcaacgtgtc gtcgcccggc ggccagagct atcacttctc ttcttcctac ggcgcgggca
     1201 aagccggcct ggaccgactc accgccgaca tggccatcga gctcgagccc aaaggggtgg
     1261 ccgcggtggt gctgtacccg ggctccgtcg ccacggagtt catccaggac cacgcggaag
     1321 cccaggggat ggatctttcc gaatcccaga cgcccctcta cgtcgggcgg tgcgccgttg
     1381 cgatgctggc cgccgacgac ctcatgtcac gcagcgggtc gatccagtgg gtggaagacc
     1441 tcggcgagga gttcgacatc ctcgacgagc acggcaagcg cccgcccggc tacgctcagc
     1501 ggc
//
LOCUS       DBNHQR010000394         1474 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1556706, whole genome shotgun sequence.
ACCESSION   DBNHQR010000394 DBNHQR010000000
VERSION     DBNHQR010000394.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1474)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1556706"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="AC16VR_08940"
     CDS             <1..619
                     /locus_tag="AC16VR_08940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6011320.1"
                     /translation="ERGDRYPQFRENVEIVLKAWRSDRLTYHGRFHCYEDIEVLPKPL
                     QDPIPVWMAASSVDSVRWAAQEGFSILMDPHSSMPDIAAKQQIYHETLIAHGHDPEGR
                     DLPVARLLAIAPTDAQAREVAEAGAQWTTGSYAKQPLALKKKDNEQDPVQRYIDEIIL
                     WGSPERVADRLLEQQEDGHLNYLLCAPLSNQSFRLFTDEVLPRIA"
     gene            complement(659..>1474)
                     /locus_tag="AC16VR_08945"
     CDS             complement(659..>1474)
                     /locus_tag="AC16VR_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011321.1"
                     /translation="NLTHDEYRAGHVGYHNSFGRRLNNIRGYLLNVRANATIEETLGT
                     DLVQSITLDEPPGFDDLWDGWGQLLFDSLDDYLAARSPARDMAGPDGLQMDPGVAGVG
                     GDFEYLYGGSPFQFHLAENVVVPVVRPERKLFKLVQFVKRPDGLEPELFRAYLSGRYA
                     RAAATMGGLCGLVVNFRTGVDVMTHFFAPDAEGFTPEGTAIRERFFSYWDAMIEYWLE
                     SPADFVAGRATGSAAENMAALEKTLMSRSFFREVDETVAVLPNRAPPPPFYHR"
BASE COUNT          259 a          501 c          495 g          219 t
ORIGIN      
        1 cgagcggggc gaccgctacc cccagttccg ggagaacgtc gagatcgttc tcaaggcctg
       61 gcgctcggac aggctcacct accacggccg cttccactgc tacgaggaca tcgaggtgct
      121 gcccaagccg ctccaggacc cgatcccggt ctggatggcg gcttcttccg tcgacagcgt
      181 tcgctgggcc gcgcaggagg gattttccat tctcatggat ccgcactcgt ccatgcccga
      241 catcgccgcc aagcagcaga tctaccacga gacgctgatc gcccacggcc acgatccgga
      301 gggccgcgac ctgccggtgg cgcggctgct cgccatcgcg ccgacggacg cccaggcccg
      361 cgaggtggcc gaggcaggcg ctcagtggac cacgggcagc tacgccaagc agccgctggc
      421 cctgaagaaa aaggacaacg agcaggaccc ggtgcagcgc tacatcgacg agatcatcct
      481 ctggggcagc cccgagcggg tggccgatcg gctgctggag cagcaagagg acgggcacct
      541 gaactatctg ctctgcgcgc cgctgagcaa ccagtcgttc cggctcttca ccgacgaggt
      601 cctgccccgc atcgcctgat cggcaggcgc gcgctcggca agcgcacgga gcgacgaatc
      661 agcgatggta gaagggcggc ggcggtgccc gattcggcag cacggccacg gtttcgtcca
      721 cttcccggaa aaagctgcgg ctcatcagcg tcttttcgag cgcggccatg ttctccgccg
      781 cgctgcccgt tgcgcggccg gcaacgaagt cggccgggct ctccagccag tactcgatca
      841 tggcgtccca gtaagaaaag aaacgctcgc ggatggccgt gccctccggg gtgaaccctt
      901 cggcatcggg cgcgaagaaa tgggtcatca cgtcgacgcc cgtccggaag ttcacgacga
      961 gcccgcagag gccgcccatg gtcgccgccg cccgcgcgta gcgccccgaa aggtaggcgc
     1021 gaaacagctc gggctccagc ccgtcggggc gctttacgaa ctgcacgagc ttgaacagct
     1081 tgcgctcggg cctgaccacc gggaccacca cgttctcggc caggtgaaac tgaaacggcg
     1141 agccgccgta gagatactcg aagtccccgc cgacgccggc cacacccggg tccatctgca
     1201 ggccgtccgg gccggccatg tcccgggcgg gagaacgggc ggccaggtag tcgtcgaggg
     1261 agtcgaacag cagctggccc cagccgtccc acagatcgtc gaagccgggc ggctcgtcca
     1321 gggtgatgct ttgcaccagg tcggtgccga gcgtctcctc gatggtggcg ttggcgcgca
     1381 cgttcaggag gtagccgcgg atgttgttga gccggcggcc gaaggagttg tggtaaccga
     1441 cgtgtcccgc acggtactcg tcgtgggtga ggtt
//
LOCUS       DBNHQR010000395         1936 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1176284, whole genome shotgun sequence.
ACCESSION   DBNHQR010000395 DBNHQR010000000
VERSION     DBNHQR010000395.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1176284"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /locus_tag="AC16VR_08950"
     CDS             <1..1263
                     /locus_tag="AC16VR_08950"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HIR6011322.1"
                     /translation="RAPGGGLDVEVAPPTLATEALLMRSARAAGVPEPEVYYVLEPED
                     GLGEGFVMEWLEGEALGARIVRARELDGVRPQLAYECGRVMAKIHAIDLDRAGLRERL
                     ETLTPEELLHRSWDRYKDFPTPQPMIDFTARWLLDNMPATFDMALVHNDFRNGNFMVD
                     ANGIVAVLDWEVAHIGDPMRDLGWICTNSWRFGRSDRPVGGFGEYADLFRGYEDESGV
                     AVDPERVKFWEVMGSFWWAVGCLGMAEHYRTGPDKTVERPAIGRRSSECQVDCVNLII
                     PGDVELVAATDLEDCTDMPRVDELLVSVRDYLRADVMAATSGRTNFLARVAGNSLDIV
                     LRELATGAEARFREQVRLEALLGRSGDLDALRWELVHRLRSGEMALDDVALTDHLRTT
                     VVNQIAIDQPRYSGFKTALARAGIEVPE"
     gene            1260..1748
                     /locus_tag="AC16VR_08955"
     CDS             1260..1748
                     /locus_tag="AC16VR_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011323.1"
                     /translation="MNIRTDAIVRLRDHLLSSDLGAAGQVPDEQPGDAVRLRVEPFAE
                     TMFLVMLADAEQAVSEHDALVVAVDMLTDGLMGREAVDALLAEFAERVRREGREDCVA
                     RIGARLSADRDDREMAFTLAAVIALADDRVAVEENEVLDLMRTYYGISDRRMEAILGT
                     LV"
     gene            complement(1745..>1936)
                     /locus_tag="AC16VR_08960"
     CDS             complement(1745..>1936)
                     /locus_tag="AC16VR_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011324.1"
                     /translation="HLKTKCGQERPWRREHDGILYVNAARVPRIYSGSDDVMRHHVSL
                     HIHEQGIEIDELEVPQGLG"
BASE COUNT          307 a          577 c          705 g          347 t
ORIGIN      
        1 cgggctcccg ggggcggctt ggacgtggag gttgcgccac cgacgctggc caccgaagcc
       61 ctgctgatgc gcagtgcccg cgccgccggg gtcccggagc ccgaggtcta ttacgttctg
      121 gagccggagg acggcctggg cgaaggtttc gtgatggagt ggctggaggg cgaagcgctt
      181 ggcgcgcgca tcgtccgggc gcgcgagctc gacggcgttc ggccgcagct cgcttacgag
      241 tgcgggcgag tgatggcgaa gattcacgcc atcgacctgg accgtgccgg gctcagggag
      301 cggctggaaa ctctgactcc ggaggagctg ctgcatcgca gctgggatcg ctacaaggac
      361 tttcccacgc ctcagccgat gatcgacttc acggcgcgct ggctgctcga caacatgccg
      421 gcgacgttcg acatggcgct cgtccacaac gattttcgca acggcaattt catggtggac
      481 gcgaacggga tcgtcgccgt gctcgactgg gaggtcgccc acatcggcga tcccatgcgc
      541 gacctcggct ggatctgcac caactcctgg cgtttcggac gcagcgaccg gccggtgggt
      601 ggtttcggcg agtacgccga tctgttccgc ggctacgagg acgagtccgg cgtggcagtg
      661 gaccccgagc gcgtgaagtt ctgggaggtg atggggtcgt tctggtgggc ggtcggctgt
      721 ctgggcatgg ccgagcacta ccgtaccggc cccgacaaga ccgtggagcg gccggccatc
      781 ggccggcgct cgtcggagtg ccaggtggat tgcgtcaacc tgatcattcc tggtgacgtg
      841 gagctggtgg cggccacgga tcttgaggat tgcacggaca tgccccgggt cgacgagctg
      901 ctggtgagcg tgcgcgatta cctgcgcgcc gacgtcatgg cggctacgag cgggcggacg
      961 aactttctgg cacgggtggc tggcaactcc ctggacatcg tgctgcgaga gctggcgacg
     1021 ggagccgagg cgaggttccg tgagcaggtc cggctggaag cgctgctggg gcgttcgggg
     1081 gatctggacg ccctgcgctg ggagctcgtc caccggctgc gatccggcga gatggcgctc
     1141 gacgacgttg ccctcacgga tcacctccgc accaccgtgg tgaaccagat cgccatcgat
     1201 cagcccaggt actctggttt caaaacggcg ctggcaagag ccggaatcga ggtgcccgaa
     1261 tgaacattcg aaccgacgcc attgtgcggc tgcgcgatca cctgctctcc agcgatctcg
     1321 gggctgccgg ccaagttccc gatgagcagc ccggcgatgc ggtccgcctg cgggtagagc
     1381 cttttgccga gaccatgttt ctggtgatgc tggcggatgc cgaacaggcc gtttcggaac
     1441 acgacgccct ggtggttgcg gtcgacatgc tcaccgatgg tctgatgggc cgcgaagccg
     1501 tggacgcgct gctggccgaa ttcgctgagc gcgttcgtcg ggaaggaaga gaagattgcg
     1561 tggcgcggat cggagctcgg ttgagcgcgg accgggacga tcgggaaatg gcgtttacgc
     1621 tcgccgccgt catcgcgctg gcagatgatc gtgtcgcggt ggaagagaac gaggtgctcg
     1681 atctgatgcg gacctactac gggatctcgg accgacgcat ggaagccata ctcgggaccc
     1741 tggtttagcc cagcccctgg gggacctcca actcgtcgat ctcgatgccc tgttcgtgaa
     1801 tgtgcagcga gacgtggtgg cgcatgacgt cgtcgctgcc ggagtagatc cgcggaaccc
     1861 gggcggcgtt gacgtagagg atcccgtcgt gctcgcggcg ccaggggcgc tcctggccgc
     1921 acttggtctt gagatg
//
LOCUS       DBNHQR010000396         4490 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1113995, whole genome shotgun sequence.
ACCESSION   DBNHQR010000396 DBNHQR010000000
VERSION     DBNHQR010000396.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4490)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1113995"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="AC16VR_08965"
     CDS             complement(<1..762)
                     /locus_tag="AC16VR_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011325.1"
                     /translation="MRIIVIVACVAIVLAGALFGAGLYFSAPVFDERVSEPPDGAFDI
                     LPVSEALTFARTKDGRLLLVRSADADAIEAVDLGAALDTVATDPLAILRTHGYQRVAA
                     RLTGVAQAVPLEQLGMPFDPGYPHIAAGTNFRAHAEEVGLDEGPFLFPKLTRATAWDA
                     RVPARTRLDYEVELCAVALGVYSADGPAPLGFVLCNDFTDRWALLKEIDLDTPMGQSG
                     FPDAKGGAGMLPIGPFLLVPSRDADFHRRLEIGLAV"
     gene            complement(770..1252)
                     /locus_tag="AC16VR_08970"
     CDS             complement(770..1252)
                     /locus_tag="AC16VR_08970"
                     /inference="COORDINATES: protein motif:HMM:NF042724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6640 family protein"
                     /protein_id="HIR6011326.1"
                     /translation="MPRSFRAELMPVDNPFDQNLIPRILMSLMALAIAIGPMFADFNK
                     THATNPLWPPHARFHVVWQVLTQAGISVVALVLLWRASPDYVLHLYLAAALIYVWLLT
                     FFMTLASIKVYQGALRDVNGIEPFRFNILGKVREVDTNLFGVVLFTALNTVAALLIRG
                     "
     gene            complement(1230..1898)
                     /locus_tag="AC16VR_08975"
     CDS             complement(1230..1898)
                     /locus_tag="AC16VR_08975"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6011327.1"
                     /translation="METFFRQQAIGFNKRLRTRHALIDSAIDVFSEKGFEDASISEIA
                     AVAGLANGTFYNHFRDKDELAAASAAAITLEIAKQLDERMSDLEAGVSRVVAASFAFL
                     RLASEHKPWGMVLVEQFQRRPGEAWAAMQYMRADIQLALEQRGLDVVLDDFLLEQLGA
                     LMIAALRRQLSEGLQEAQLQRTCEHVLRVLGLTPEEARREVEGVAGHVLLGDELSLAE
                     IFPR"
     gene            1980..3605
                     /locus_tag="AC16VR_08980"
     CDS             1980..3605
                     /locus_tag="AC16VR_08980"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR6011328.1"
                     /translation="MLSTQVLIIGAGPVGLLSAIVLDRLGISSVLVERRLDRLQAPKA
                     HAVNPRTLEICQRFGVPADEVRAAGASIEDGGQVHFVDVLNGTCFGSLPYERQDDGAR
                     AFTPWPLVNIPQPRFEEFLSQRLAQCANTSLLRGLTATNTEESEGGVTTKLSAGGLSS
                     DWDIRSDYVIAADGAGSQTRERLGVGMTGPEALQHWIMIHFEADLTELTSKRPGLLYF
                     CMSPQARGVFIGYERDRSWVFMHAYDPSTQSREDFSPEICRRLVGAAAGQSIDPLTVR
                     NVSTWTMSAQVADSYRAGRVFLAGDAAHRFPPTGGLGLNTGIADAHNLAWKLAMVLHE
                     QADPSLLDTYDGERRPIAQINSHQSVTNSAKLLHLFGALYGPDPAATPEHYASACADP
                     TAFPEISQNVEIQRHHFDSFNLQLGYRYGATVDPDTMDISRYVPAFEVGDHLPHVRLQ
                     DGSWLVGKLPVEGFSLITGPKAQAWAKVACPVFSEDVDFKADQRAFFEQAGLHRDGAL
                     LLRPDGHICARWLKAPEDPQAAVDEQVNEATGR"
     gene            3607..4086
                     /locus_tag="AC16VR_08985"
     CDS             3607..4086
                     /locus_tag="AC16VR_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020798267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6011329.1"
                     /translation="MDLGINGRHAIVCASSRGLGKACEAPDILVTNNAGPPPGAFADW
                     GPDEWQAALEANLLAPTFMIQGVIEGMKERRFGRIVNITSAMVKSPHAAMGLSTAARS
                     GLTFEDARQAIANTINAKRFGQPEELGDACAYLCSAQAGFICGQNLQLDGGSYAGLI"
     gene            4070..>4490
                     /locus_tag="AC16VR_08990"
     CDS             4070..>4490
                     /locus_tag="AC16VR_08990"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase family protein"
                     /protein_id="HIR6011330.1"
                     /translation="MRASSDRHGYTRRAAWFGCCMALSFASAALGSTQTGPNIVLILA
                     DDLGWNDVGYHGSEISTPNIDALATQGVVLNQFHSQPTCTPTRAALMTGKSPQRMGIY
                     RQFAKISTEGLPVGEKTLADHLRAAGYQTWLTGKWHLG"
BASE COUNT          890 a         1457 c         1394 g          749 t
ORIGIN      
        1 gaccgcgagg ccgatttcca ggcgccggtg aaagtccgca tcgcgactgg gcaccagcag
       61 gaatggcccg atgggcagca tgcccgcccc gcccttggcg tcgggaaatc cggattggcc
      121 catcggcgtg tccaggtcga tttccttcag gagcgcccag cgatcggtaa agtcgttgca
      181 cagcacgaaa ccgagcgggg caggcccgtc ggcggagtag acgccgaggg caacggcgca
      241 gagctcgacc tcgtaatcca ggcgcgtacg tgccggtacc cgtgcgtccc acgctgtagc
      301 ccgggtcagc ttgggaaaca ggaagggccc ctcgtccagg cccacttcct cggcatgggc
      361 gcggaaattg gtccccgcag cgatgtgcgg gtagccgggg tcgaacggca tgccgagctg
      421 ctccagcgga accgcttggg ccacgcctgt cagccttgcc gccacccgct ggtagccgtg
      481 tgtgcgcaga atggccaacg ggtctgttgc caccgtgtcg agcgcggcgc cgaggtcgac
      541 ggcctctatg gcatccgcgt cggcgctgcg cacaagcagc agccggccat ccttcgtgcg
      601 ggcaaaggtc agcgcctcgc tgacgggcag aatgtcgaag gcaccatcgg ggggttctga
      661 gacgcgctcg tcgaaaaccg gtgcagaaaa atagaggccg gcaccaaaga gcgccccagc
      721 gagcacaatc gccacgcagg cgacgatgac gatgatcctc atgactcgtt cacccccgga
      781 tcagcagcgc ggcaaccgtg ttcagcgcgg tgaaaagaac cacaccgaag agattcgtgt
      841 ccacctcgcg caccttgccc aatatgttga accggaaggg ttcgatgccg ttcacatccc
      901 gaagcgcgcc ctggtaaacc ttaatcgagg cgagggtcat gaagaaggtg aggagccaga
      961 catagatgag cgcggccgcg aggtagaggt gcaacacata gtcaggggac gctcgccaca
     1021 gcagcaccag ggcgaccacg ctgataccag cctgggtcag cacctgccag accacgtgaa
     1081 agcgcgcatg tggcggccac agcgggttcg tggcgtgggt tttgttgaaa tcggcaaaca
     1141 tgggaccgat cgctatggcc aaggccatca ggctcatgag gatgcgggga atcagattct
     1201 ggtcgaacgg gttgtccacg ggcatcaact cagcgcggaa agatctcggc aagactcagt
     1261 tcgtctccga gcagcacatg cccggcgacg ccctccactt cgcgcctggc ctcctcgggc
     1321 gtcagcccca gaacgcgcag gacgtgctca caggtgcgct gtaactgagc ctcctgcaag
     1381 ccctcagaga gctgccttcg caaggcggcg atcatgagcg cgccaagctg ctcgagcagg
     1441 aaatcatcaa gaaccacgtc gagaccacgc tgctccaacg ccagctgtat gtccgcccgc
     1501 atgtactgca tggctgccca cgcctcgcca ggacggcgct gaaactgctc caccaggacc
     1561 atgccccagg gtttgtgctc cgaagcgagc cgaagaaagg caaacgacgc cgccacgacg
     1621 cgcgacaccc ctgcctcgag atcgctcatg cgctcgtcga gctgcttggc gatctccagc
     1681 gtgatggccg ccgcggaagc cgcggccagc tcgtctttgt ccctgaaatg attgtagaaa
     1741 gtgccgttgg ccagacccgc gacagcggcg atctcgctga tgctcgcatc ttcgaaaccc
     1801 ttctccgaga acacgtcaat ggcactgtcg atcagcgcgt gacgtgttcg aagtcgcttg
     1861 ttgaaaccga tggcctgctg ccggaagaaa gtctccatgg ctcggcatcc taggatcaat
     1921 gaattgacat gtcaatcata tttgtattta ctctcatttt ttaacgaaca ctcatatcca
     1981 tgctgtcaac ccaggttctc atcatcggcg ccggaccggt gggcctcttg agcgcgatcg
     2041 ttctcgaccg gctggggatt tccagcgtgc tcgtggagcg acggctcgat cgccttcagg
     2101 cgccgaaggc acacgcggtc aatccgcgca ccctggagat ctgtcagcgc ttcggggtac
     2161 cggccgacga ggtgcgcgcg gccggcgcga gcattgagga cggcgggcag gtgcacttcg
     2221 tggacgtgct caacggcacc tgtttcggaa gcctgcccta cgaacgccag gacgatgggg
     2281 ccagagcgtt cacgccttgg ccgttggtga acattcccca accccggttc gaagagttcc
     2341 tgagccagcg tctggcgcag tgcgcgaaca cctcgctgct gcgcgggctg accgcgacca
     2401 acacggaaga atccgaaggc ggtgtcacca ccaagctgag tgccggcggt ctcagcagcg
     2461 attgggacat acgatcggac tatgtcatcg ccgccgacgg cgccggctcc cagacacggg
     2521 agcgcctcgg cgttggcatg accggcccgg aagccctgca gcactggatc atgatccact
     2581 tcgaagcgga cctcaccgag ctgaccagta agcggcctgg attgctgtac ttctgcatgt
     2641 cccctcaggc caggggcgtc ttcattggct acgagcgcga ccgcagctgg gtattcatgc
     2701 atgcctacga tcccagcacg caaagccggg aagatttcag cccggaaatc tgcagacggc
     2761 ttgttggagc cgcggccggc caatccatcg acccgttgac cgtcaggaac gtgagcacgt
     2821 ggacgatgtc tgcgcaggtc gccgacagct atcgggcggg tcgggtcttc ctggccgggg
     2881 atgccgcgca ccgctttcca cccaccggcg gtctgggcct gaataccggc attgccgatg
     2941 cgcacaacct ggcctggaag ctggcgatgg tgttgcacga gcaggccgat ccgagcctcc
     3001 tggacacata cgatggagaa cgccgaccca tcgcgcagat caacagccac cagagcgtga
     3061 cgaactccgc aaagctgctg catctgttcg gcgcgctgta cgggcccgat ccagcggcga
     3121 cgcccgagca ctatgcttcg gcatgcgccg atcccaccgc gttcccggaa atcagccaga
     3181 acgtcgagat ccaacgacat cacttcgaca gcttcaacct gcagctcggc tatcgatacg
     3241 gcgcaaccgt cgacccggat accatggata tcagccgcta cgtacccgcc ttcgaagttg
     3301 gcgaccatct tccccacgta aggcttcagg acggatcctg gttggtcggg aagctgccgg
     3361 tagagggctt ttccctgatc accggaccga aagctcaggc ctgggcgaag gtcgcttgcc
     3421 cggtgttctc ggaagacgtc gacttcaaag ccgatcaacg cgcctttttc gagcaggcag
     3481 ggctccaccg cgacggggcg ctcctgctgc gcccggatgg ccatatctgc gcgcggtggc
     3541 tgaaagcccc cgaagatccg caagcggcgg tcgacgagca agtgaacgag gcaacgggac
     3601 gctgacatgg accttggaat caacggtcgt cacgccatcg tctgcgcgtc atcgcgcggt
     3661 ttgggcaagg cctgcgaagc acccgacatc ctggtcacca acaacgctgg cccgcccccc
     3721 ggcgcgtttg ccgactgggg ccccgacgaa tggcaggctg cactagaggc gaacctccta
     3781 gcacccacct tcatgatcca gggggtcatc gagggcatga aagagcgtcg tttcgggcgc
     3841 atcgtcaaca tcacctccgc gatggtgaag tccccccacg cagccatggg gttgtccacg
     3901 gcagcgcgca gcggcctgac ctttgaggat gcgcgacagg ccatcgcaaa caccatcaat
     3961 gccaaacgct tcggtcagcc ggaagagctc ggggatgcct gcgcctatct gtgcagcgca
     4021 caggccgggt tcatctgcgg ccagaatcta cagctggacg gcggttccta tgcgggcctc
     4081 atctgaccgt cacggctaca cgcgccgggc agcgtggttc ggctgctgca tggctttgtc
     4141 gttcgcctcg gctgctctgg gctcgaccca aacggggcca aacatcgtcc tgatcctggc
     4201 cgacgacctg gggtggaacg atgtcggcta ccacggcagc gagatttcga caccgaacat
     4261 cgacgccctc gccacgcagg gcgtggtgct gaatcagttt cacagccagc ccacctgcac
     4321 gcccacgcgg gccgcgctca tgaccgggaa gtcgccccaa aggatgggca tctaccggca
     4381 gttcgcgaag atcagcaccg aaggtctccc ggtcggcgag aagacgctgg cagatcacct
     4441 ccgagcggcc ggctaccaga cctggctcac cggaaagtgg catctggggc
//
LOCUS       DBNHQR010000397         2833 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_417002, whole genome shotgun sequence.
ACCESSION   DBNHQR010000397 DBNHQR010000000
VERSION     DBNHQR010000397.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_417002"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="AC16VR_08995"
     CDS             <1..291
                     /locus_tag="AC16VR_08995"
                     /inference="COORDINATES: protein motif:HMM:NF015438.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HIR6011331.1"
                     /translation="IRRFRPNFHVQTPDDMTGLAENEWVGKTIRVGTAVMRITAETPR
                     CGMTVRPQAEIEFDKSILRTIVRESHQNLGVGAHCIEDGRISVGDALVIVDA"
     gene            904..>2833
                     /locus_tag="AC16VR_09000"
     CDS             904..>2833
                     /locus_tag="AC16VR_09000"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF014869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HIR6011332.1"
                     /translation="MAREPLGRTLAMLPLLVAMTATAADRQDLRTNAHAISTLHKQTD
                     QGGSPNYHAAFGLGQQEGLTAVSATTDANNVTHTRYRQTLRGVPVWGEQIIISREASG
                     AVRGARGGLIEGLSAHQVPARPTVAGAAALSATKNRIAAGMSQNPVFERESAELVIYL
                     HDGTPRLSWAVSFFADVPGGGEPTRPTVLLDATTGEVIFEYEGLTHAEGTGPGGNAKV
                     GGYHYGSSEPAMDVAASGTTYTMNNANLKTVNLNHGTSGSTAYSYQGPENVHKEINGA
                     YSPLNDAHFFGGVVYDMYNAWVGTPPLTFQLTMRVHYGNNYENAFWNGSAMTFGDGAS
                     TFYPLVSLDVSAHEVSHGFTEQNSNLVYSGQSGGINEAFSDMSGEAAEFYMRGSNDWL
                     VGADIFKSPSGALRYMYDPTLDGRSIGSANDYVNGMDVHYSSGVFNKAFYLLATTGGW
                     GVQKAFQTFARANQLYWSSGETFRTAAMGVQDAASDLGFSTADVEAAFSAVGVNLGDV
                     CPAEQVPELGNGASSGEFGASAGEWRCFRLSVTAGGTDASFVLAKTAKGRGGDADLYV
                     QHAALPDTVRYDCRSVSSTSSESCAIATPLEGDWYVGVYAYSSYPGVTLTGSFTDPSG
                     SGGGGGDGGGSGSTDADSD"
BASE COUNT          556 a          955 c          885 g          437 t
ORIGIN      
        1 attcgccgct ttcgaccaaa tttccatgtt cagacgccgg acgacatgac gggcttggcc
       61 gagaacgagt gggtcggcaa gaccatcagg gtcggaaccg ccgtcatgag gatcactgcg
      121 gagacaccgc gttgcggcat gacggtgagg cctcaggccg agattgagtt cgacaagtcc
      181 atactgcgca cgatcgtcag agagtcacac cagaatctcg gcgtcggcgc gcactgcatc
      241 gaggatggcc ggatcagcgt cggcgacgcg ctggtgatcg tggacgcttg aatcatgggc
      301 gttctgctcg atcgcccaca acgccctcct gctcttggag atcttaaaaa aaacgggccc
      361 agccgataag accgctgatc ggccggatgc ctaaatccct accggtggca ggcgtccgcg
      421 ccgcaagcgg cccggactgc tgctggtcgc gccgttgccg gagaccgcac agggttggga
      481 tcacatcgcg cctgcgtagg acctttcccg ctttcaggtc tcccacattt tcaaaggtac
      541 ggtccacgaa tccatccccc tgctgagccg gctgcgcagc accctaatcg cggacctgat
      601 cggcctgccg gatgaacaca acctggtggt cgcgctggcg gaagtgggcg actgccggct
      661 cgctgatgct gcggcgctgc cgccctgctc ggatccggtc gtgaacgact tcacgccaat
      721 caagggaagt gcgtgacgcg ggtcacagtt tgggcaggag ggccgcggcg ggcccgccga
      781 aaagcaagga aaacattaca tctgcgcaac caaccgcccg caaaacccac cccatcgccc
      841 aagtaccatg ctagagattc atttctcatg aaaagcaacc ccagcagtaa caaggagaac
      901 cccatggcac gcgaacccct tggccgcacc ctggccatgc tgccgctcct ggtggccatg
      961 accgctaccg cagccgaccg gcaggatctc agaacgaatg cccacgccat cagcaccctt
     1021 cacaagcaga ccgaccaagg gggcagcccc aactaccacg ccgctttcgg cctcgggcag
     1081 caggaaggcc tcacagccgt cagcgccacc accgacgcaa acaacgtcac ccacacccgt
     1141 taccgtcaga ccctgcgagg ggtgccggtg tggggcgagc agatcatcat cagccgggag
     1201 gcttccgggg cagtgcgcgg cgcacggggc ggcctgatcg aggggctgtc agcgcaccag
     1261 gtgcccgcac gcccgacggt cgccggggcg gcggcgttga gcgccaccaa gaaccgcatc
     1321 gcggccggca tgtcacagaa ccccgtcttc gagcgggaga gcgccgagct ggtgatctat
     1381 cttcacgacg gcacaccccg cctgagctgg gcggtgagct tttttgccga cgtgcccggc
     1441 ggcggcgagc cgacccggcc gacggtgctc ttagacgcca cgaccggcga agtcatcttc
     1501 gagtatgagg gcctgaccca cgctgaaggc acggggcccg gcggtaatgc aaaggtgggc
     1561 ggctaccact acggttccag cgagcctgcc atggacgttg ccgccagcgg caccacctac
     1621 accatgaaca acgccaacct gaaaacggtg aacctcaacc acggaacctc gggctctacg
     1681 gcctacagct atcaaggccc ggagaacgtg cacaaggaaa tcaacggcgc ttacagcccg
     1741 ctgaacgacg cccacttctt tggcggcgtg gtctacgaca tgtacaacgc ctgggtgggc
     1801 acgccgccgc tgacctttca gctgaccatg cgcgtgcact acggcaacaa ctacgagaac
     1861 gccttctgga acggctcagc gatgaccttc ggcgacggtg ccagcacctt ctaccccctg
     1921 gtgagcctgg acgtctcggc acacgaggtc agccacggct ttaccgagca gaactcgaac
     1981 ctggtctact ccggacagtc gggcggcatc aacgaagcgt tctccgacat gtccggcgag
     2041 gcggccgagt tctacatgcg cgggtcgaat gactggctgg tcggcgcaga catcttcaag
     2101 agcccaagcg gcgccctgcg ctacatgtac gaccccaccc tggatggccg ctccatcggc
     2161 agcgccaacg actacgtcaa cggcatggac gttcactact ccagcggcgt gttcaacaag
     2221 gccttctacc tgctcgcaac cacaggcgga tggggcgtcc agaaggcgtt ccagacgttc
     2281 gcccgtgcaa accagcttta ctggtcctcc ggagaaacgt tccgcaccgc cgccatgggc
     2341 gtgcaggatg cggcctcgga tctgggcttc tccactgccg acgtcgaggc tgccttcagc
     2401 gccgtgggcg tgaatctggg tgacgtgtgc ccggccgagc aggtcccaga gctgggcaac
     2461 ggcgcgagca gcggggagtt cggcgccagc gccggcgagt ggcgctgctt caggctgagc
     2521 gtcaccgcag gcgggaccga tgcctccttc gtgctggcaa agacggccaa gggtaggggc
     2581 ggcgacgccg atctctatgt ccagcacgca gcgctgccgg acaccgttcg ctacgactgc
     2641 cgctcggtga gcagcaccag cagcgagagc tgcgccatcg ccacgcctct cgaaggcgac
     2701 tggtacgtgg gcgtctacgc ctacagctcc taccccggcg tgacgctcac cggcagcttt
     2761 accgacccca gcgggtcggg cggcggtgga ggagacgggg gcggatcggg gtcgaccgac
     2821 gctgactccg acg
//
LOCUS       DBNHQR010000398         3104 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1430356, whole genome shotgun sequence.
ACCESSION   DBNHQR010000398 DBNHQR010000000
VERSION     DBNHQR010000398.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1430356"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1235)
                     /locus_tag="AC16VR_09005"
     CDS             complement(<1..1235)
                     /locus_tag="AC16VR_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011333.1"
                     /translation="MSAARCLATWLLLATLPGLASAAPGGELLGGSLGRWLDETAGPE
                     LAETLARHPKFKGETVRFVPMLDGRAEPAGNRLVQAVEQRLTRHLIREGGVRIAWERD
                     RQPCAAPEEAPYLLGIEIQQRGPDIYHLNIAMVDVDEGVWVSGVSLSWKGRLLPTERT
                     ALDTRANGGPPGSMQSPLPLSDSSVIGELMLAGLGCAIPAGLDGSLYVAMPVESDLSG
                     VAQALTRQLSVRPLAALTIDRAQASWEMALTARPLGNGTDELVATLRDLRREGSAQQI
                     ASVVISRPRSERPGPVTPLGMPAALLSGLSYETARSRGVCQSSTGPGGCIEVSFDLLR
                     PAYLLVVSSRDERLESQVCGRSLRIAEPGPRRYRIRVPSGAATASRPDAGVFVLAGEK
                     RSVPPRKARQSNPGPGACPG"
     gene            complement(1222..1923)
                     /locus_tag="AC16VR_09010"
     CDS             complement(1222..1923)
                     /locus_tag="AC16VR_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011334.1"
                     /translation="MTFEPVNHAADGDGLQTDVMRFMAIIAFCLIAVLALVKNAEPAV
                     DPQPAPSVQQPVGMAQASELAAPEVEPRAAVRPARPQPVPPVATGTPQERPAEVEDVE
                     DVEKVPGEPAAPHGLALRFASDRDFLRLIETGRVRVFAFQPDDPGGTALGLGRNLEFF
                     ASPPPGRVYELQADTIPSRVTASLARAAGNPGGFSWGVRLPVELEASIRSWIDQDVIG
                     ELIIDRYAQVRHVGS"
     gene            complement(1920..2528)
                     /locus_tag="AC16VR_09015"
     CDS             complement(1920..2528)
                     /locus_tag="AC16VR_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011335.1"
                     /translation="MKGVAEFVCGAGAGAGLALVALGLSGGAVAADAAPVSDWLQLNL
                     GHSAWLFALVLTVYAANLHRLSRLLAGGPDLKVVVKLDQLSDVWINLFVGIGVIWTAV
                     GMRSALQAALGDPGGALSGSAGTVLHKLVDGGILLALTTTIVGGVGGYVMRLVKTMVV
                     GARLQTFYSGVSRADLRELVGLGERIEARLARLDPQEPAVVA"
     gene            complement(2529..3065)
                     /locus_tag="AC16VR_09020"
     CDS             complement(2529..3065)
                     /locus_tag="AC16VR_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011336.1"
                     /translation="MFRRIFVPAFFISLVAGCTSTETVDACAVRFQDDLERSMSLAED
                     RLENGCEYRFDGYFQSLLAIAAENPDSRNPMLFSDHLMRVNDMGVISKRQAELLYNRY
                     FNVKFVSLRGDYNTCSQTCPMRDQVMSDMRAELHDKELGLMRASADRASFYRADTLLK
                     EADLVLEATCRACGADGR"
BASE COUNT          533 a         1077 c         1037 g          457 t
ORIGIN      
        1 cccggacagg ctccgggtcc ggggttgctc tgccgcgcct tgcggggggg gacgctgcgc
       61 ttctcccccg caagaacgaa cacgcccgca tccgggcggc tggccgtcgc cgccccggag
      121 ggaacccgga ttcggtatcg ccggggcccg ggctcggcga tacggaggct gcgtccgcag
      181 acctgagatt ccagacgctc atcgcgactg gagacgacca gcaggtaggc cggacgcagc
      241 agatcgaaag acacctcgat gcagccgccg ggtccggtcg acgactggca gacgccccgg
      301 ctgcgcgcgg tttcgtagct caatccggaa agcagcgcag ccggcatgcc cagcggcgtc
      361 accgggccag ggcgctcgct gcgcggcctg gatatcacca cggaagcgat ctgctgagcg
      421 gagccctccc ggcgcaggtc acgaagggtc gcgacgagct cgtctgttcc gttgcccagc
      481 ggtctcgccg tcagcgccat ctcccagctc gcctgggcgc gatcaatggt caacgcggcc
      541 agaggccgga cgctgagctg ccgggtgagt gcctgagcga cgccggacag atcggactcc
      601 acgggcatgg ccacgtagag cgagccgtcg agcccggcag gaatcgcgca gcccaggccc
      661 gccagcatga gctcgccgat gacgctgctg tcgctcaaag gcagcgggct ctgcatcgac
      721 ccgggcggac cgccgttcgc tcgggtatcg agcgccgtcc gctccgttgg gagcaggcgg
      781 cccttccagg agaggctgac gccgctgacc cagacgcctt cgtccacgtc gaccatggcg
      841 atgttgaggt gatagatgtc cgggccccgc tgctgaatct ctatgcccag cagatagggg
      901 gcctcctcag gcgcagcaca gggctgccgg tcgcgctccc aggcgattcg cacccctcct
      961 tcgcggatga ggtgacgggt caaccgctgc tcgaccgcct gaacgagccg gttgccggcc
     1021 ggttccgcgc gaccgtccag catgggcacg aagcgtacgg tctctccctt gaacttgggg
     1081 tggcgggcga gggtctcggc cagctccgga cccgccgttt cgtcgagcca tctgcccagg
     1141 gatccgccga gcagctcgcc gccaggcgcc gcggacgcca gacccggcag agtcgccagc
     1201 agtagccacg tcgccaggca cctagctgcc gacatgtcgc acctgcgcat atcggtcgat
     1261 gatcagctcc ccaatcacgt cctggtcgat ccagctgcgg atcgacgctt ccagctcgac
     1321 cggcaggcgc acgccccagg aaaagccgcc cgggttcccg gctgcccggg cgagggaagc
     1381 ggtcacgcgc gacggtatgg tgtctgcctg gagctcgtag acgcggcctg ggggcgggga
     1441 ggcaaagaac tccaggttcc ggccgaggcc gagcgcggtg ccgcccggat cgtccggctg
     1501 aaaggcgaat acacggacgc gtcccgtctc gatgaggcgc aggaaatccc gatccgaggc
     1561 aaagcgcaac gcgagcccat ggggagcggc aggttcgccc ggtaccttct caacgtcctc
     1621 aacgtcctca acttctgccg gcctctcctg gggcgttccc gtcgccaccg gcggcaccgg
     1681 ttgcgggcgc gcggggcgca cggcagcgcg aggttcgacc tccggggccg caagttccga
     1741 cgcctgcgcc atcccgacgg gttgctgcac cgacggcgcg ggctgcggat caaccgccgg
     1801 ctcggcgttt ttgacaaggg cgagcacggc tatgaggcaa aaggcaatga tcgccatgaa
     1861 ccgcatgacg tcagtctgca ggccatcgcc gtcggcggcg tgattgaccg gctcgaacgt
     1921 catgccacca ccgccggttc ctgggggtcg agacgggcca gacgggcctc gatccgctcg
     1981 ccaaggccca ccagctcgcg aaggtctgca cggctaacgc cggagtagaa ggtctgcagg
     2041 cgggcgccga ccaccatcgt cttgaccaga cgcatgacgt agcctccgac cccgccgacg
     2101 atggtcgtcg tcaacgccag cagaatacct ccgtcgacga gcttgtgaag gaccgtgcct
     2161 gcggagcccg acagcgcgcc gccgggatca ccaagggccg cctgcagcgc gctgcgcatg
     2221 ccgacggccg tccatatgac cccgataccg acgaacaggt tgatccagac gtcggagagc
     2281 tgatcgagct tgacgaccac cttcaggtcc gggccgccgg cgagcagtcg gctcagccgg
     2341 tgcaggttgg cggcatagac cgtcagcacc agagcgaaga gccaggcgga gtgccccagg
     2401 ttgagctgca gccagtcgct cacgggtgca gcgtccgcgg caacagcccc gcccgagagg
     2461 cccagggcca ccagcgcgag ccccgcgccc gcgcccgctc cgcacacgaa ctccgcgacg
     2521 cctttcattc agcgcccgtc cgcgccgcaa gcccgacagg tcgcttccag gacgagatcg
     2581 gcttccttga gcagggtgtc cgcccggtag aagctcgccc ggtccgcgct ggcccgcatc
     2641 aggccaagct ccttgtcgtg cagctccgcg cgcatgtcgg acatgacctg atcgcgcatg
     2701 gggcaggtct gcgagcacgt gttgtagtcg ccgcgcaggg agacgaactt gacgttgaag
     2761 taacggttgt agagcaactc cgcctggcgc ttgctgatga cgcccatgtc gttcacgcgc
     2821 atcaggtgat cgctgaagag catgggattc ctcgagtccg gattttccgc ggcgatcgcc
     2881 agcagggact gaaagtagcc atcgaagcgg tactcgcatc cgttctccag ccgatcctcg
     2941 gcgaggctca tgctgcgctc gaggtcgtcc tgaaatcgca ccgcacaggc atccacggtc
     3001 tccgtgctgg tgcagccagc gacgagcgag atgaaaaagg ccggaacaaa tatcctacga
     3061 aacatgtctt tctcctgatc gaatcggcgc tacatggcca gcgg
//
LOCUS       DBNHQR010000399         1780 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1430454, whole genome shotgun sequence.
ACCESSION   DBNHQR010000399 DBNHQR010000000
VERSION     DBNHQR010000399.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1430454"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="AC16VR_09025"
     CDS             <1..450
                     /locus_tag="AC16VR_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011337.1"
                     /translation="TWYGLFTETGERPESVDVMQHLWTGAWPSNRSPAVRSVRLDGRR
                     AGRDIVLVPGQTYPILARVRDPEGDALRYRWSVKSESRARQVGGDLEGAIADVDGLID
                     EPSSSRAQLTAPEEPGAYRVFVYAYDGNGGAAHANVPFLVRSPAGAD"
     gene            544..1770
                     /locus_tag="AC16VR_09030"
     CDS             544..1770
                     /locus_tag="AC16VR_09030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6011338.1"
                     /translation="MIDFEIPAETKAIRAKAREFVQKECVAAEAQCTADNFDQVLSEL
                     RGKARAQGLWCPFIPEEHGGMGLKPLANALVQMELGESFLGALALNTQGPDDATMLTL
                     LEHGTDHQKEKFLKPLLDGQKRICYSMTEKAAGADATGMQTAAVLEGDNWVLNGEKWF
                     SSSADVADIAVVMAKTDPEAPRHERYSTFIVELPNPGYQILRNIDTMQPHTELGLKLG
                     HSHSEVKIENLVVPRENLLGGRGKGFAMGQHRLAYGRLRHGMHNVAMAQRALDLAAGH
                     VVNRETFGKRLADRQGVRWMLADCAAEIYKARLMLLHIAYKAERGMDLRQENGIAKVF
                     LAHMVHQVVDTALQLHGALGYSHDTPLASWYTSIRSQRLVDGPDEVHRWRTGANVIKA
                     YEEHGTTAPACGGELL"
BASE COUNT          329 a          578 c          616 g          257 t
ORIGIN      
        1 acgtggtacg ggctgttcac cgaaaccggt gagagaccgg agtcggtcga cgtcatgcaa
       61 cacctctgga ccggcgcatg gccgagcaat cgcagccccg cggtcagatc ggttcgtctc
      121 gacggccgca gggcggggcg ggacatcgtc ctcgttccgg ggcagacgta tcccattctc
      181 gcccgggtgc gcgatccgga aggggacgcc ctgcgctatc gctggagcgt caagtcagag
      241 agccgggcca ggcaggtcgg aggagacctc gagggggcga tcgccgacgt ggacgggctg
      301 atcgacgaac cgtcgtcgag ccgcgcgcag ctgacggccc ccgaagagcc cggggcgtat
      361 cgcgtcttcg tctacgcata cgacggcaat ggcggcgcgg cgcacgccaa cgtccccttc
      421 ctcgtccgtt ctccggctgg agccgactga acgcaccacc cctttcgggg cctgccgtgg
      481 ttcccgcgcc cgtgctcctg atatagtcac gacagacacg aacctgcata gggaaaggga
      541 ccaatgatcg atttcgagat accggccgag accaaagcca tccgcgccaa ggcgcgcgag
      601 ttcgtgcaga aggaatgcgt ggcggcggag gcgcaatgca cggcggataa tttcgaccag
      661 gtgctttcgg agctcagagg caaggcccgc gcccagggcc tgtggtgtcc gttcatcccc
      721 gaggagcacg ggggcatggg gctgaagccg ctggccaacg ccctggttca gatggagctc
      781 ggcgagagct ttcttggcgc gctcgcgctc aacacccagg gtccggatga cgccaccatg
      841 ctcacgctgc tcgagcacgg caccgaccac cagaaagaaa agttcttgaa gcccctgctg
      901 gacggccaga agcgcatctg ctactcgatg acggagaagg cggcgggtgc ggacgccacg
      961 ggcatgcaga ccgcggccgt tctggagggc gacaactggg tgctcaacgg cgagaagtgg
     1021 ttcagctcgt cggccgacgt cgccgacatc gccgtggtga tggccaagac ggacccggag
     1081 gcgccgcgcc acgagcgcta cagcacgttc atcgtcgagc tgcccaaccc gggctatcag
     1141 atcctgcgca acatcgacac catgcagccc cacactgagc tgggcctcaa gctcggccac
     1201 tctcactccg aggtgaagat cgaaaacctc gtggtaccgc gcgagaacct cctcggcggc
     1261 cggggcaagg gctttgccat gggccagcac cggcttgcct acggccggct gcgtcacggc
     1321 atgcacaacg tggccatggc gcagcgggcg ctggatctgg ccgccgggca cgtggtcaat
     1381 cgggagacct tcggcaaacg gctcgccgat aggcagggcg ttcgctggat gctggccgac
     1441 tgcgcggcgg agatctacaa ggcccggctg atgcttctgc acatcgccta caaggccgag
     1501 cgaggcatgg acttgaggca ggagaacggg atcgccaagg tgttcctcgc gcacatggtg
     1561 catcaggtgg tggacaccgc gctgcagctg cacggggcgc tgggctacag ccacgacacg
     1621 ccgctggcga gctggtacac gagcatccgc agccagcggc tcgtggacgg cccggacgag
     1681 gtgcaccgct ggcgcacggg cgccaacgtg atcaaggcct acgaggagca cggtaccacg
     1741 gcgcccgcct gcggcggcga gctgctgtga gcgaggtccg
//
LOCUS       DBNHQR010000400         2949 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1241209, whole genome shotgun sequence.
ACCESSION   DBNHQR010000400 DBNHQR010000000
VERSION     DBNHQR010000400.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1241209"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="AC16VR_09035"
     CDS             <1..554
                     /locus_tag="AC16VR_09035"
                     /inference="COORDINATES: protein motif:HMM:NF014954.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR6011339.1"
                     /translation="NNPAQRDGMIGGWQVGWPVVKAQFPDLIPSAALEAGDRMPDAVG
                     RLLEEMCREPVCLIHTDVRLDNVFFGADEIMLVDWQAVCTSAPEQDVAYFVTQSVAPE
                     VRAQDDLVKFYHGELVSHGIDYGLERCRERYRVSALYLLCFAVVIAGTLDLANERGAQ
                     LGRTLLLNAFAALDEMDAFDLLN"
     gene            577..1359
                     /locus_tag="AC16VR_09040"
     CDS             577..1359
                     /locus_tag="AC16VR_09040"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6011340.1"
                     /translation="MSRLDGRVAVVTGGSRGIGAAIVRAFVDEGAMVAFNGRSAESGA
                     ALMEALGEPERVWFCQGSVTVTADLEKLIDGAVDRFGKLDILVNNAGGSNAMQPVAEL
                     DEAAWESDLRFNLTSTFLGTKLALKHMLPAGYGTVINISSVEGKMGVPVMAAYTACKH
                     GVNGFTKAVAAEVGRAGITVNAICPGLILTDAVTGGGPALAESMGMTFDEMVENVFKA
                     KTLTGELNTVEQIGETAVFLASPAGRGITGAQISVDGGMTPY"
     gene            complement(1392..1979)
                     /locus_tag="AC16VR_09045"
     CDS             complement(1392..1979)
                     /locus_tag="AC16VR_09045"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR6011341.1"
                     /translation="MSSEAGLRHLPNLISSLRLLCAPVLIWLTVQGLSDVFAWILIAA
                     WLSDLADGWLARSFGWVSRIGAMLDSVADIALTLATLYGIWVFHNDVFAVHGWLIWLV
                     LGLWVTVNSIALVRYGRPASFHARLAQVGMALFGVFVLTLFFYDFVGWIYYLCGAVCL
                     AAGVENLAMIYLLDDWTPNVRGGLMAALRRRQGDR"
     gene            complement(1976..>2949)
                     /locus_tag="AC16VR_09050"
     CDS             complement(1976..>2949)
                     /locus_tag="AC16VR_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6011342.1"
                     /translation="IDRDRAGDLGIEMNDIGVALATLLGGNYVNRFSLAGRSYEVIPQ
                     VERRFRASHEMLNDNYLRAASGELVPLSSFISFEHSIEPTRRSQFQQLNAVTINGVMA
                     PGVSLGDAITFMERTAAELFPREFSYDYVGESRQFKQQGSALVVTFFLSIIVIYLVLA
                     AQFESWRDPLIILISVPMSIAGALAFVMLGFASINLYTQVGLITLIGLIAKNGILIVD
                     FANRQQLVAGLSKRAAVEQAAAVRLRPILMTTVAMLMAMVPLLIATGPGAVSRFQIGL
                     VIATGLGIGTLFTLFVVPSVYLLLARDRTDPAQRDAATPRPTPGLEP"
BASE COUNT          544 a          919 c         1009 g          477 t
ORIGIN      
        1 acaacaaccc cgcccagcgc gacgggatga tcggcggctg gcaggtgggt tggcccgtgg
       61 tcaaggccca gtttccggat ctgatcccgt cggccgccct cgaggcgggg gacaggatgc
      121 ccgacgccgt ggggcggctg ctggaggaaa tgtgccggga acccgtttgc ctcatccaca
      181 ccgacgtgcg tctggacaac gtcttcttcg gcgctgacga gatcatgctg gtggactggc
      241 aggctgtgtg cacctctgcc cccgagcagg acgttgccta ctttgtcacc caaagcgttg
      301 cgcccgaggt gcgggcccag gacgatctgg tgaagttcta tcacggcgag ctcgtcagcc
      361 acggcatcga ctacggcctc gagcgctgcc gggagcgtta ccgggtttca gccctttacc
      421 tgctctgctt tgcggtggtc atcgccggaa ccctggatct cgccaacgaa aggggcgccc
      481 agctcggccg aacgctgctg cttaacgcct ttgcagcgct ggacgagatg gacgccttcg
      541 acctgctgaa ctaaccgcgt ttgcccggga ggtaacatga gtcgattgga cggccgggtg
      601 gccgtggtca cgggcggcag tcgcggcatc ggcgccgcga tcgttcgggc attcgtcgac
      661 gagggcgcca tggtggcgtt caacggccgc tccgctgagt ctggcgctgc gctgatggaa
      721 gcccttggtg agcccgagcg ggtctggttc tgtcagggct ccgtgaccgt cacggcggat
      781 ctcgagaagc tcatcgacgg cgcggtcgat cgcttcggca agctggacat cctggtgaac
      841 aatgccgggg gcagcaacgc catgcagccg gttgcggagc tggacgaggc cgcctgggag
      901 agcgatctga gattcaacct cacctcgacg ttcctgggca ccaagctggc gctcaagcac
      961 atgcttccgg ccgggtacgg cacggtcatc aacatctcct ccgtggaggg caagatgggg
     1021 gtgccggtca tggccgccta cacggcctgc aagcacggcg tgaacggctt caccaaggcc
     1081 gtggcagcgg aggtggggcg tgcgggcatc accgtgaacg ccatctgccc gggcctgatc
     1141 ctcaccgacg cggtaaccgg cggcggccct gctctggcgg aatccatggg catgacgttc
     1201 gatgagatgg tggaaaacgt cttcaaggcg aaaacgctga cgggtgagct gaacacggtg
     1261 gagcagatcg gcgagaccgc cgtttttctg gcctcaccgg ccggccgcgg gatcacgggt
     1321 gcccagatct ccgtcgacgg gggcatgacg ccctactagc agcggcatcg ggcggttgcc
     1381 ggtgcctgca atcagcggtc gccctggcgg cgccgcagcg cggccatgag gccgcctcgc
     1441 acgttcggcg tccagtcgtc gagcaggtag atcatggcga ggttttccac gcccgcagcc
     1501 aggcagacgg cgccgcacag gtagtagatc cagccgacga agtcgtagaa gaagagcgtc
     1561 aaaacgaaaa ccccgaagag ggccatgccg acctgtgcga gcctcgcgtg gaagctggcc
     1621 ggtcgtccgt accggacgag cgcgatggaa ttgactgtca cccacaggcc gagaaccagc
     1681 cagatcagcc agccgtgcac ggcgaagacg tcgttgtgaa agacccagat gccgtaaagc
     1741 gtggccagcg tcaatgcgat gtcggcgacg gaatccagca tggcgccaat cctgctgacc
     1801 cagccgaacg agcgggccag ccacccgtct gcaagatcgc tgagccaggc tgcgatcagg
     1861 atccaggcga atacgtccga cagcccctgc accgtgagcc agatcagcac gggcgcgcag
     1921 agaagacgca ggctcgagat caggttgggc aggtgacgca ggcctgcctc tgaactcatg
     1981 gctcgagccc cggcgtcggg cggggcgttg ctgcatcgcg ctgcgcagga tcggttcggt
     2041 cccttgccag caaaagatag acgctcggca cgacgaacag cgtgaacaag gtgccgatgc
     2101 cgagccccgt ggcgatcacg aggccgatct ggaagcggct caccgcccct ggcccggtgg
     2161 cgatgagcag cggcaccatg gccatgagca tggctaccgt ggtcatcagg atcggccgca
     2221 accggaccgc cgcggcctgc tccaccgctg cccgcttgct gagcccggcg acgagctgct
     2281 ggcggttggc gaagtcgacg atgagtatgc cgttcttcgc gatcaggccg atgagcgtga
     2341 tcagccccac ctgggtgtag aggttgatgg aggcgaatcc gagcatgacg aacgccagcg
     2401 cgccggcgat ggacatgggc accgagatga ggatgatcag cggatcccgc cagctctcga
     2461 actgggccgc cagcaccaga tagatgacga tgatcgacag aaagaacgtc accaccagag
     2521 cgctgccctg ctgcttgaac tgacgggact cgcccacgta gtcgtaggag aactcgcggg
     2581 gaaacagctc tgcggcggtg cgctccatga aggtgatggc atccccgagg gatacccccg
     2641 gcgccatgac gccgttgatg gtcacggcgt tcagctgctg aaactggctg cgacgggtgg
     2701 gctcgatgga gtgctcgaag ctgatgaagc tcgacagggg aacgagctcg ccggacgctg
     2761 ctcgcaggta gttgtcgttc agcatctcgt ggctggcgcg gaagcggcgc tcgacctggg
     2821 ggatcacctc gtagctccgg cctgcgaggc tgaaccggtt gacgtagttg ccgcccagca
     2881 gcgtcgcgag cgcgacgccg atgtcgttca tctcgatacc gagatcacct gcccggtccc
     2941 gatcgatca
//
LOCUS       DBNHQR010000401         5415 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_100701, whole genome shotgun sequence.
ACCESSION   DBNHQR010000401 DBNHQR010000000
VERSION     DBNHQR010000401.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5415)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5415)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5415
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_100701"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="AC16VR_09055"
     CDS             complement(<1..255)
                     /locus_tag="AC16VR_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="binds to the HflBKC complex which modulates FtsH
                     activity; Derived by automated computational analysis
                     using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HIR6011343.1"
                     /translation="MANYEVSSVRAAAAPAIEINKVLRNTYMLLGLTLAFSAVVATAA
                     MIADAPYLGFLPTLVGFFGLLFIVHKTANSPWGLFWTFAFT"
     gene            409..2829
                     /locus_tag="AC16VR_09060"
     CDS             409..2829
                     /locus_tag="AC16VR_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007978235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcvT family protein"
                     /protein_id="HIR6011344.1"
                     /translation="MTLPERTDVVIIGGGIIGASIAYHLAELGKLDVCLIERGRLTCG
                     TTWHAAGLVAELRASPNLTRLARYSAELYESLESAGEHTGFRRVGALTLACQPERVRE
                     LKRQAAMARQNQVSCNWLEPPELAERWPDLDLSGVLGAVFMPRDGQTSPVDTTRALAN
                     RARQNGARVLEGVSVTRIEVASGAVAGVTTQRGHIACERVIVCAGLWSRALAADAGAI
                     LPIYPAEHFYAVSEPIDAASRLPIVRSPDQGIYLKPDAGRLLVGCFESAAKPLAPESL
                     PDEFAFDELPFDLDHFAPYLQNAVDRLPALGNTGIRTWFNGPESFTPDGRYIIGPLPE
                     IRGLFAACGMNSIGIQSAGGIGQVMARWVTDGRPPMDLWEVDARRFMPFQNETDYLVE
                     RTSEALGLLYAMHWPYRQHESARPIRQSPLHERLAAAGACFGELAGWERANWYARPGQ
                     APRYEYSYGRQNWFPNAAQEHRAAREAVALFDQSSFAKYEVSGPDACRLLNRLGTSDI
                     DVAIGRVVYCQWLNERGGIEADVTVTRLGPETFWVVSAAPCQRRDLAWLAEHSAGFQA
                     DVKDVTDQHAVIGVMGPDSRRLLTSLADADFGHDAFPFATSQPIAIANTALRATRITY
                     VGALGWELYVPRAEAVTVYDAIAGAGPVHAGYHAMDSLRLEKAYRHWGHDITDEDTPI
                     EAGLSFTAAWDKPEGFIGREALLEQRADGVTKRLALFVLNDPDPLLYHDEPIWWNGRP
                     VGRITSGAYGHTLGRSMGFGYVAADAPFPRQALREGRFQIEIEDTLVDAACHLRAPFD
                     PDNREILR"
     gene            complement(2836..3381)
                     /locus_tag="AC16VR_09065"
     CDS             complement(2836..3381)
                     /locus_tag="AC16VR_09065"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR6011345.1"
                     /translation="MRLAETLWFVAFVILSVAVPGLAAGSELEVGDPAPGFTLEGSDG
                     RTYSLSDYVGRQAVVIAWFPKAYTRGCTIECKSLAENGHLIRRFDVSYFMASVDPIED
                     NIGFAEQQKADFPLLSDPTKETARAYGVLSFAGFAKRHTIYIAKDGTIAAIDTDVRPA
                     TSAEDIATRLGELGVAAAPES"
     gene            complement(3422..4468)
                     /locus_tag="AC16VR_09070"
     CDS             complement(3422..4468)
                     /locus_tag="AC16VR_09070"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6011346.1"
                     /translation="MDQEHESLMSHGRFDNPVIAALAGFKDRFREQPLASRLTDADRF
                     DGKRVLITGANSGLGYALAVDVARRGGSVTMACRSEIPEAGQRAIGESGSQNIEMRHC
                     DLSRMETLHGFCRSAAADGLRFDVVILNAATTLPRARPAPCGQDEMFVVNYLANFALV
                     HLLIDHGLLVPDARVQPRIVVISSDSHQGSSAIDYEEFGRYFDYGVRKAIANYSYFKL
                     LLNTFATELSRRLNSGDGIRVQVNVICPGPVNSNIIKAAPWALRLVLRAIFRVIFKSP
                     AEAARPVIYMAASSDFAGRTNDYLHMFSEKPMDSKVYDESAGSRLWRESAELWARIDD
                     KAADALSAIDKPGL"
     gene            4650..>5415
                     /locus_tag="AC16VR_09075"
     CDS             4650..>5415
                     /locus_tag="AC16VR_09075"
                     /inference="COORDINATES: protein motif:HMM:NF014954.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase family protein"
                     /protein_id="HIR6011347.1"
                     /translation="MAIHPLPPTAADIGPVWLTEVLHGSGGLSAEGRVKEVTWEKLGE
                     GAGMMSDLARLRMTYEGDAGSAPATLVVKQPSAVENNRLVAVKYNTYAREVRYFAELD
                     PVCAAQGPGIHLSEIDPDNNFVILMDDLSDYHIGDQIVGADLPQSEVCVDELAKLHGS
                     FWAAADDLDWLPHIHGSEHAGNMISGAQIGWDTMLKIFGEFVPDAINGERERFLDSIG
                     PLQHRLDTPPITVLHGDFRMDNLFFGQNPEQHPLIML"
BASE COUNT          989 a         1792 c         1719 g          915 t
ORIGIN      
        1 cgtgaaggcg aaggtccaga agaggcccca ggggctattg gccgtcttgt gcacgatgaa
       61 cagaagaccg aagaagccga ccagggttgg gagaaaaccg aggtaggggg catctgcgat
      121 catcgccgcg gtggcgacca ccgcgctgaa cgccagcgtg aggcccagca gcatgtaggt
      181 attgcgcaga accttgttga tctcgatggc cggagcggcg gccgccctga ccgaagacac
      241 ttcgtaattt gccattttct ttactgaatc cttactgcta atcgttccca gggcagcaac
      301 acaccgcccg aaccaagtat ataagtactt accgagccca tttcaagttt gcctgttatc
      361 agtttgacca cgcaacataa ggccggtttc atgccccgga ccccgaaatt gacactaccc
      421 gagcgcaccg acgtggtgat tatcggcggc ggcatcatcg gcgcgagcat cgcctaccac
      481 ctcgccgagc tgggcaagct cgacgtctgc ctgatcgagc gcggacggct cacctgcgga
      541 acgacctggc acgctgccgg gctcgtggcc gagttgcgag cctcaccgaa cctgacccgc
      601 ctggcccggt actcggccga gctgtacgaa tccctggagt cggcgggcga gcataccggc
      661 ttccggcggg tgggtgcgct gaccctggcg tgccagccgg agagggttcg ggagctcaag
      721 cggcaggcgg ccatggcgcg ccagaatcag gtctcctgca actggctcga gccgccggag
      781 ctggccgagc gctggcctga tctggatctg tccggcgtcc tgggcgccgt gttcatgccc
      841 cgtgacggcc agacgagccc cgtagacacg acccgtgcgc tggcgaaccg ggcacgacag
      901 aacggcgcgc gcgtgctgga gggggtctcc gtgacccgca tcgaagtcgc ctcgggagcg
      961 gtcgcggggg taaccacaca gcggggccac atcgcctgcg agcgggtgat cgtctgcgcc
     1021 ggtctatggt ccagggccct ggccgccgat gcaggcgcca tcctgccgat ttatcctgcg
     1081 gagcatttct acgcggtctc cgaacctatc gatgcggcca gccggcttcc catcgtgcgc
     1141 tcgccggacc agggcatcta tctgaaaccc gatgcagggc ggctgctggt gggctgcttc
     1201 gagtccgccg ccaagcccct tgcgcccgag tctctaccgg acgaattcgc cttcgacgag
     1261 ctgcccttcg acctcgacca cttcgcgccg tacctgcaga acgccgtgga ccgtctgccg
     1321 gcattgggca acacgggcat acgcacctgg ttcaacggcc cggagagctt cacgccggac
     1381 ggccgctaca tcatcggccc cctccccgag atccggggcc tgttcgcagc ctgcgggatg
     1441 aactccatcg ggatccagtc cgccggcggc atcggccagg tcatggcacg ctgggtcacc
     1501 gacggccgcc ctcccatgga tctttgggag gtggatgcac gccgtttcat gccttttcag
     1561 aacgagaccg actacctcgt cgagcgaacg tccgaggctc tgggcctgct ctacgccatg
     1621 cattggccgt atcgccagca cgaaagcgca agacccatcc gccagtcgcc attgcacgag
     1681 cggctggccg ccgccggtgc ctgtttcggc gagctggccg ggtgggaacg ggccaactgg
     1741 tacgcccgac cgggccaggc gccccgctac gagtacagct acggcaggca gaactggttc
     1801 ccaaatgctg cccaggagca tcgcgcggcc agggaagccg tcgccctttt cgatcagtcg
     1861 agctttgcca agtacgaggt ttccgggccg gacgcgtgcc gcctgctcaa ccgcctcggc
     1921 acgtccgaca tcgacgtcgc catcggccgg gtcgtgtact gccagtggct caacgagcgg
     1981 ggcgggatcg aagcggacgt gaccgtgacc cggcttggtc ctgaaacgtt ctgggtcgta
     2041 tccgctgcgc cctgccagcg ccgggacctg gcatggctag ccgagcacag cgccggtttc
     2101 caggccgacg tgaaagacgt taccgaccag cacgccgtca tcggcgtcat gggacctgac
     2161 agccgcagac tgctgacatc gctcgcggac gcggacttcg gccacgacgc ctttcccttc
     2221 gccacctcac agccaatcgc catcgcaaac accgccctgc gcgccacgcg catcacctac
     2281 gtcggcgccc tgggctggga gctctacgtg ccccgggccg aagccgtcac ggtgtacgac
     2341 gccatcgccg gggcaggccc cgttcacgcc ggctatcacg ccatggactc gctgcggctg
     2401 gagaaagcgt accggcactg gggtcacgac atcaccgacg aggacacgcc catcgaggcg
     2461 ggactcagct tcaccgccgc ctgggacaag ccggaggggt tcatcggccg cgaagcgctg
     2521 ctcgagcagc gggcggacgg cgtgacgaag cggctcgccc tgttcgttct gaacgacccc
     2581 gaccccctgc tgtaccacga cgaaccgatc tggtggaacg gcaggcccgt gggccgtatc
     2641 acatcaggcg cctacggcca cacgctcgga cggtcgatgg gtttcggcta cgtcgcagcc
     2701 gatgcgccct tcccccgcca ggcgctgagg gaaggacgat ttcagatcga gatcgaggac
     2761 acgctggtgg acgcagcctg ccacctcagg gcgcccttcg acccggacaa ccgggaaata
     2821 ctgcgctgag cgggactagc tctcgggcgc ggcggccacc ccaagctcac ccagccgggt
     2881 cgcgatgtcc tcggcagacg tggccggacg gacgtcggta tcgatggcgg cgatggtgcc
     2941 gtccttcgcg atgtaaatgg tgtgacgctt cgcgaacccg gcgaagctga ggacgccgta
     3001 ggcccgggcc gtttccttgg tgggatcgct taagagcgga aagtcggcct tctgctgctc
     3061 ggcaaaaccg atgttgtcct cgatgggatc aacgctggcc atgaagtagc tgacgtcgaa
     3121 ccggcgtatg aggtgaccgt tctcggccag cgacttgcac tcgatggtgc agccgcgggt
     3181 ataggctttg gggaaccagg cgattacgac ggcctgtcgg ccgacgtagt cggacaacga
     3241 ataggtccga ccgtcgctgc cctccagcgt gaatccgggc gcggggtcac ccacttcgag
     3301 ctcgctgccg gcggccagcc cgggcacggc gactgataaa atgacgaacg caacgaacca
     3361 gagagtctcg gctaaacgca cgacggccag cctccacgca aaccagagag tgcagagcct
     3421 atcacagtcc gggcttgtcg atggctgaca atgcgtcggc tgctttgtca tcaatcctgg
     3481 cccacagctc ggcggactcg cgccacagcc gggaacccgc gctttcgtcg tataccttcg
     3541 aatccatggg tttctcgctg aacatgtgaa gatagtcgtt ggtcctgccc gcgaagtcgg
     3601 agcttgccgc catgtagatg accggtcgcg cggcttccgc cggcgacttg aagatcacac
     3661 ggaagatggc acgcaggacc agccgcagcg cccagggcgc tgccttgatg atgttcgagt
     3721 tcacgggacc gggacagatg acgttgacct gaacccggat accgtcgcct gagttgagcc
     3781 ggcggctgag ctccgtggca aaggtgttga gcagcagctt gaagtagctg tagttggcga
     3841 tggccttgcg aacaccgtag tcgaaatagc gtccgaactc ttcgtagtcg atggccgagc
     3901 tgccctggtg gctgtcggag gagatgacca cgatgcgagg ctggacgcgg gcgtccggga
     3961 cgagcagccc gtggtcgatc agcagatgca caagcgcaaa attcgccagg taattcacta
     4021 caaacatctc gtcctggccg cagggtgccg gccgggccct cggaagggtg gtggccgcat
     4081 tgagaatgac cacgtcgaag cgaagcccgt ccgcggccgc cgaacggcag aacccgtgca
     4141 gcgtctccat cctgctcagg tcgcagtgac gcatctcgat gttctgcgat ccgctctcgc
     4201 cgatcgcgcg ctgacctgcc tcgggaatct ccgagcggca cgccatggtc acgctgccgc
     4261 cgcgccgggc cacgtcgacg gccagcgcat acccgagacc cgaatttgcg ccggttatca
     4321 gaacccgctt gccgtcgaag cgatcggcat cggtcagccg ggatgccagc ggctgttcgc
     4381 ggaagcgatc tttgaagccc gccagcgcgg cgatcaccgg attgtcaaag cggccgtgcg
     4441 acatcagcga ctcgtgctcc tgatccattt cccctcgccc gcacccgggc ttggggtatg
     4501 cgggtgaaag actacgtcct gccaaccacg gcgcaaagct gattcgtggc ccgggccgtc
     4561 accaaccgcg gcgtccaagg aatggtttag tccgaacacc ctgttatcct cttcgtttcc
     4621 aggtttcccg tgcatccagg aggacgatga tggcgataca tccactgccc ccaaccgcag
     4681 ccgacatcgg cccggtgtgg ctcaccgagg ttctgcacgg cagcggcggg ctgtccgccg
     4741 aaggccgcgt caaagaagtc acctgggaaa agctcggtga aggcgccggg atgatgagcg
     4801 acctcgcccg cctgaggatg acctatgaag gcgatgccgg cagcgcgccg gccactctgg
     4861 tggtcaaaca gccatcggcg gtcgagaaca atcggctcgt cgcggtcaag tacaacacct
     4921 acgcccggga agtccgctac ttcgccgagc tcgatcccgt ctgcgcggca caaggccccg
     4981 gcatacacct ttcggagatc gatcccgaca acaacttcgt gatcctgatg gacgatcttt
     5041 ccgactacca catcggcgat cagatcgttg gtgcggatct accacagtcc gaagtgtgcg
     5101 ttgacgagct ggccaagctg catgggtcgt tctgggccgc ggccgacgac ctggattggc
     5161 tgccccacat ccacggcagc gagcacgccg gcaacatgat ctccggggcg cagattggct
     5221 gggacaccat gctgaagatc ttcggagaat tcgtgcccga cgccatcaac ggtgagcgcg
     5281 agcgtttcct ggatagcatc ggtccgcttc agcatcgcct ggacacgccg ccgatcaccg
     5341 tcctgcacgg cgacttccgg atggacaacc tctttttcgg ccagaacccc gagcagcacc
     5401 cgctgatcat gctgg
//
LOCUS       DBNHQR010000402         6145 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1114691, whole genome shotgun sequence.
ACCESSION   DBNHQR010000402 DBNHQR010000000
VERSION     DBNHQR010000402.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6145)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6145)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6145
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1114691"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="AC16VR_09080"
     CDS             <1..902
                     /locus_tag="AC16VR_09080"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TauD/TfdA dioxygenase family protein"
                     /protein_id="HIR6011348.1"
                     /translation="ACHDDAGKSSTCRELCVSGGSRRSWRAMTGALGAIAPPGDALDL
                     PAAEIVQALNAHQVVAFRNQSLDPAELVAFASRLGELDVYPLAEPLPESPFVVAVLKN
                     PEDTSNFGGAWHTDTSYVREPPKFTVLYAVEIPGSGGDTLFADMYQAFAGLSPGLQRC
                     LERMQAVYTADLVHAGSGEYTAVAGQSVPAKESERATTVEHPVVRVHPETGRKALYTS
                     LIHTERFVGMTREESLPLLEYLQSVSVAAENCTRLHWQAGTLAIWDNRCVQHYPLNDY
                     PGERRLMHRVIVRGEEPVGVEPL"
     gene            986..1516
                     /locus_tag="AC16VR_09085"
     CDS             986..1516
                     /locus_tag="AC16VR_09085"
                     /inference="COORDINATES: protein motif:HMM:NF049802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1271..1273,aa:Sec)
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HIR6011349.1"
                     /translation="MVDYHDPRGEVATPVDAYLLSVDLESSNDPRVAFLANGFPDSDV
                     FLEQLAEVMSEKLTSLRAEHYNKGNASIAAPQDMLEEIREGCVAAVAAYGHUGSCTTG
                     TVRDGINMARLGIPAVALVTEDFWPQGDFIAGSLGMPDIPRVRLPHPVAGTGVANLRK
                     IAQEIAPEVFAALSRN"
     gene            1526..2587
                     /locus_tag="AC16VR_09090"
     CDS             1526..2587
                     /locus_tag="AC16VR_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011350.1"
                     /translation="MSGLFTARSEAAAVEHLHELGCTDGLPVIVPTPDRVERMVLASG
                     QDPAAVLGEMGPGKGIATIEKVAVAAVMAGCLPDYMPLVVAAVRAVIDPVFDLTEMQG
                     TTHCTAPLIIVNGPARHWCGPIASGFGALGPGHRANASIGRALRLTMINIGGARPGVS
                     DMALLGHPGKFTYCLAEDEEESPFTPLHVSRGFAAEDSVVTVIGAEAPHSVLYSVDAD
                     DPEDHEKLLGILAVGLGNLATNNAQLRNGSAVVVLNPDHAAVLANAGLSREDVAARLQ
                     ELAVHSRADIERYGKAFMNWSRDEEVFHCFQDPAQILVVMAGGSGLYSMVMPSWCAGG
                     HRNSPVSVRVELDQACEIP"
     gene            2669..3832
                     /locus_tag="AC16VR_09095"
     CDS             2669..3832
                     /locus_tag="AC16VR_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015475134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-sulfuration enzyme family protein"
                     /protein_id="HIR6011351.1"
                     /translation="MNWPKDRYGFETRAIHAGQAPDPATGAVVTPVYLTSTFQHSEPG
                     EFAGFDYSRAGNPTRKAFEDCVANLEGARFGFAFASGCIGAATVVHLLEQGDHVVACD
                     DMYGGTHRLFEQVFRPQGIDFSYLDLSSADRLRGALRPETRMVWIESPTNPLMKLVDI
                     AAVATIAREHGAMLMVDNTFMTPYLQRPLELGADMVLHSTTKYINGHSDLIGGLVVTD
                     DEQLAERLDFLNKTMGGIQGAFDSYLCLRSVKTLAVRMQAHERNARAVAEMLASHPKV
                     EWVGYPGLSSHPQHELARRQASGFGGMVAFRVTGGLAAARRLLGAVEVFTLAESLGGV
                     ESLIEHPGLMTHASLPADRRAALGIDDGLIRVSVGIETTGDLLADLEAALAAA"
     gene            3832..4260
                     /locus_tag="AC16VR_09100"
     CDS             3832..4260
                     /locus_tag="AC16VR_09100"
                     /inference="COORDINATES: protein motif:HMM:NF051508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7832 domain-containing protein"
                     /protein_id="HIR6011352.1"
                     /translation="MIYDDLDRHLPAAGALERAVVPMGMYLAWCVNLQLTGSALEEEA
                     GTLLLRVRYREKTGSELLLAACGGVLDGRWLNDAGRRFTDGYYGGYLDDFRSVFGSDV
                     YGVEDDWDHYDRLAPLLTRRYLGESAKTDRGARRWWKFWQ"
     gene            4279..4692
                     /locus_tag="AC16VR_09105"
     CDS             4279..4692
                     /locus_tag="AC16VR_09105"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6011353.1"
                     /translation="MSGRSNDGGGREFRVTDVRVYRPYPDEVPWDLLDRQAPADPAGD
                     GVQMRIAKLGDHVVGAYVIEPVDPLTFRIVDLVVAEGWRRRGIGRWLLGHAIGLAESR
                     GAREVRVTNPSPRRFFASIGFLDADAGMRFPITPE"
     gene            complement(4679..5839)
                     /locus_tag="AC16VR_09110"
     CDS             complement(4679..5839)
                     /locus_tag="AC16VR_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012506.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HIR6011354.1"
                     /translation="MVADIIAALRCGPFANDKGANDMSSDTLPKGLEGVEVDETGISL
                     VDGERGVLSYRGIDVDELVDKPFDEVAALVATGRLDPDFGARLAASAELSEREAGLVR
                     SLPRDVHPMHVVQGLTPLLDRDGAFADFGDAAQGFAVAAKLPALIATHMLGTAVAGYP
                     TLGHAERFLAQIAAPQTDIARRAFNVTQILQIEHGFNASTFAARVAASTLAPVENALS
                     AGFGTLHGRLHGGADQAALEVAEQVGTAAAAAAFVDRCIADGTRVMGMGHREYRVLDP
                     RARHIKELARSLAAGTPHEKTFETLAAIEDRFRERMAEKGKALHANVEFYKGVVYRVL
                     GMTPAFFTTGFAMARVFGYLAHFIESREDNRLIRPKARYTGPPVGAVRLTPA"
     gene            5938..>6145
                     /locus_tag="AC16VR_09115"
     CDS             5938..>6145
                     /locus_tag="AC16VR_09115"
                     /inference="COORDINATES: protein motif:HMM:NF012354.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR6011355.1"
                     /translation="MQIERHEIRIFSAVVEEGGFSRAAERLNITQSAVSQAVANLEHK
                     LDARLLERRGKPELTEAGKRLFAFT"
BASE COUNT          989 a         2017 c         2094 g         1045 t
ORIGIN      
        1 aagcatgcca cgatgatgcg ggaaaaagca gcacatgccg ggagctctgc gtgagcggcg
       61 gatcccggcg tagctggcgc gccatgacgg gcgcgctcgg cgcgatcgcg ccgccagggg
      121 atgcccttga tctgccggcc gccgagatcg tccaggcact caacgcccat caggtggtcg
      181 cgttcaggaa tcagtctctc gacccggcgg agctggtggc gttcgcttcc cggctgggtg
      241 agctcgacgt ttacccactc gcagagcccc ttcccgaatc gcctttcgtg gtcgccgtgc
      301 tcaagaaccc ggaggatacg agcaacttcg gcggggcatg gcatacggac acctcctacg
      361 tccgcgagcc gcccaagttc accgtgctct acgccgtgga aattcccggc agtggcggtg
      421 acacgctgtt cgcggacatg taccaggcgt ttgccggcct gtcccccggg ctgcagaggt
      481 gccttgaacg catgcaggcc gtgtatacgg ccgacctggt gcacgcgggc agcggggagt
      541 acacggcggt ggcggggcag agcgttccgg cgaaggagag cgagcgggcc accacggtgg
      601 agcacccggt ggtgcgcgtt catccggaaa ccgggcgcaa agcgctctac accagcctga
      661 tccacaccga gcggttcgtc ggcatgacgc gcgaggaaag ccttcctctg ctggagtacc
      721 tgcagagcgt ttcggtggcc gctgaaaact gcacccggct ccactggcag gccggcacgc
      781 tggccatttg ggacaatcgc tgcgtgcagc actatccgct gaacgactat cccggagagc
      841 ggcggctcat gcaccgggta atcgtcaggg gagaagagcc tgtgggcgtg gaaccgctct
      901 gaggcccatt gctgagctgg agttcgctgc ccattcccgt tttaatcggt gtcatcgatc
      961 agcaccctgc atcggaggtg ggtatatggt tgactatcac gacccccggg gagaggtcgc
     1021 aacgccggtg gatgcctatc tgctgagcgt cgatctcgag tccagcaacg acccgcgtgt
     1081 ggcgttcctc gccaacgggt tcccggattc agacgttttt ctcgagcagc tcgccgaagt
     1141 catgagcgaa aagctgacga gccttcgcgc cgagcactac aacaagggca acgcgtccat
     1201 cgccgcgccc caggacatgc tcgaggaaat tcgcgagggc tgcgtggcgg cggtggccgc
     1261 ctacggccac tgaggcagtt gcactaccgg caccgtgcgt gacggcatca acatggcaag
     1321 gctcggcatc ccggcggtgg cgctggtgac cgaggacttc tggcctcagg gcgacttcat
     1381 cgccggctcg ctcggcatgc ccgacattcc cagggttcgg ctgccccacc cggtggcggg
     1441 gaccggtgtc gccaacctgc gcaagattgc gcaggagata gcgcctgagg ttttcgcggc
     1501 gctgtcccgg aactagggcg aggctttgag cggattgttc acggcccgga gcgaggcggc
     1561 cgcggtcgag caccttcacg agctcggctg caccgacggc ctgcccgtca tcgtgcccac
     1621 gccggacagg gtggaacgca tggtcctggc ctccggccag gatcccgccg ccgtcctcgg
     1681 tgagatgggt ccgggcaagg gcatcgccac catcgagaag gtggcggtgg cagcggtgat
     1741 ggccggctgc ctcccggact acatgccgtt ggtggtggcc gccgtgcggg cggtcatcga
     1801 tcccgtcttc gacctcacgg agatgcaggg gaccacccac tgcacggcgc ccctgatcat
     1861 cgtcaacggg cccgcgcggc actggtgcgg tcccatcgcc agcggcttcg gcgccctggg
     1921 accggggcat cgggccaacg cctccatcgg gcgagcgctg cggctcacga tgatcaacat
     1981 cggcggcgcc cgccccggcg tctccgacat ggccctgctc ggccatcccg gaaagttcac
     2041 ctactgtctt gccgaggacg aggaggagag cccgtttacc ccccttcacg tttcccgggg
     2101 cttcgcggcc gaagacagcg tggtgaccgt catcggcgcg gaggcgccgc actccgtgct
     2161 ttacagcgtc gatgccgacg atcccgagga tcacgagaag ctgctcggca tcctcgccgt
     2221 gggcctgggc aacctggcta cgaacaacgc ccaactgcgc aacggctccg ccgtggtggt
     2281 gctaaacccg gatcatgctg ccgtgctcgc caatgcgggc ttgagtcgcg aggacgtggc
     2341 cgcccgcctg caggagctgg ccgtgcactc ccgtgccgat atcgagcgct acggcaaggc
     2401 gttcatgaac tggtccaggg acgaagaagt atttcactgc tttcaggacc ccgcgcagat
     2461 cctcgtggtg atggccggtg gcagcgggct gtattccatg gtcatgccct cctggtgcgc
     2521 gggcggccac aggaactctc cggtcagcgt cagggtcgaa ctcgaccagg cctgcgagat
     2581 cccctgacct gccgcgagcc atgccgtcgg aggtgctccg gcccggggcg atgggtcgcc
     2641 ggaggcaaag gtttcaggga gctgcagcat gaactggccc aaagacagat atggattcga
     2701 gacccgggcg attcacgcgg ggcaggcccc cgatcccgcg acgggcgccg tggtgacccc
     2761 cgtttacctg acctcgacct ttcagcactc cgagccaggc gagtttgccg gcttcgatta
     2821 ctcccgggcc ggcaacccga cccgcaaggc cttcgaggac tgcgttgcca acctggaagg
     2881 ggcgcggttc ggcttcgcct ttgcgtctgg ctgcatcggc gcggcgacgg tcgttcacct
     2941 gctcgagcag ggcgatcacg tggtggcctg tgacgacatg tatggcggca cccaccgcct
     3001 gttcgagcag gtattccggc cccaggggat cgacttcagc tacctcgatc taagctcggc
     3061 ggatcggctt cgcggggcgc ttcgaccgga gacccgcatg gtctggatcg agtcgcccac
     3121 caatcctctg atgaagctcg tggacattgc cgccgttgcg acgattgccc gcgaacacgg
     3181 cgcgatgctg atggtggaca acaccttcat gaccccttat ctccagcgcc cgctggagct
     3241 cggggccgac atggtgctgc actccaccac gaagtacatc aacggtcaca gcgacctgat
     3301 cggcgggctg gtggtcaccg acgacgagca gcttgccgag cgcctcgact ttctcaacaa
     3361 gaccatgggc ggcatccagg gcgcgttcga cagctacctt tgcctgcgca gcgtgaagac
     3421 cctggccgtt cgcatgcagg cacatgagcg aaacgcgcgg gcggttgcgg agatgctggc
     3481 gtcgcacccg aaggtggaat gggtgggcta ccctgggctg tcgagccatc cgcagcacga
     3541 gctggctcgg cggcaggcct cgggcttcgg cgggatggtc gccttccgcg tcacaggtgg
     3601 cctggctgct gcccggcgcc tgctcggcgc ggtggaggtg ttcaccctgg ccgagagcct
     3661 gggtggcgtc gagtccctca tcgagcatcc gggcctgatg acccacgcga gcctgccggc
     3721 cgatcgccga gccgcgctcg ggatcgacga cgggctcatc cgcgtcagcg tggggatcga
     3781 gacgacgggc gatctcctgg ccgatctcga agctgcgctg gctgccgcct agtgatttac
     3841 gacgatctgg accggcatct gcccgctgcc ggcgcccttg agcgcgccgt ggtgcccatg
     3901 ggcatgtacc tggcatggtg cgtcaatctt cagctgacgg gcagcgcgct ggaggaggag
     3961 gccgggaccc ttttgctgcg ggttcgctac cgggagaaaa ccggtagcga gctgctgctc
     4021 gccgcctgcg gcggcgtgct ggacggccgc tggctgaacg acgcgggccg tcgcttcaca
     4081 gacggctact acggtggata tctggacgac ttccgctcgg ttttcggatc cgacgtctac
     4141 ggcgtagagg acgactggga tcactacgat cgtcttgcgc ccctgctgac gcgccggtac
     4201 ctcggagaga gcgctaagac ggatcgcggc gcccggcgat ggtggaagtt ctggcaatga
     4261 tttggcgatg agctggcaat gagcggtagg tctaatgacg gtggcggacg ggaattccgg
     4321 gtaaccgacg tgcgcgttta ccggccttat ccagacgagg ttccctggga cctgctggat
     4381 cggcaggcac cggcggatcc cgcgggagac ggggtgcaga tgcgcatcgc gaagctcggc
     4441 gaccacgtgg tgggcgctta cgtcatcgaa cccgtcgatc cgctgacctt caggatcgtg
     4501 gacctggtgg tggccgaagg ctggcgccgg cgcgggatcg gcagatggct tctgggtcac
     4561 gccatcgggc tggcggagtc ccgtggggct cgcgaggttc gggtgaccaa tccatcccct
     4621 cggcggttct tcgccagcat cggtttcttg gacgccgatg cagggatgcg atttcccatc
     4681 acgccggagt gagcctcact gcgcccacgg gcgggcccgt gtaccgagct ttcgggcgga
     4741 tcaggcggtt gtcctcgcgg ctctcgatga agtgggccag gtagccgaac acccgggcca
     4801 tggcaaagcc cgtggtgaag aacgcgggcg tcatgccaag cacccggtag acgacgccct
     4861 tgtagaactc cacgttggcg tggagcgctt tgcccttctc ggccatccgc tcgcgaaacc
     4921 gatcctcgat ggccgctaag gtctcgaagg tcttctcgtg gggcgtgccg gcggcgaggc
     4981 tgcgcgcgag ttccttgatg tggcgcgcgc gtgggtccag cacgcggtat tcccgatgcc
     5041 ccatgcccat cacgcgggtc ccgtcggcga tgcatcggtc cacgaacgcc gccgcagccg
     5101 ccgctgtgcc cacctgctcg gcgacctcca acgccgcctg gtccgcgccg ccgtgcagac
     5161 gcccgtggag ggtcccgaag ccggcggaca gggcattttc caccggtgcg agggtgctcg
     5221 ccgcgacccg ggcggcaaag gtgctggcgt tgaagccgtg ctcgatctgc aggatctggg
     5281 tgacgttgaa ggcgcgccgg gcgatgtcgg tctgcggggc cgcgatctgg gccaggaacc
     5341 gctccgcgtg ccccaatgtc ggatagcccg ccaccgcggt gccgagcatg tgcgtcgcga
     5401 tcagcgcagg aagcttggcc gccacggcaa atccctgagc ggcgtcgccg aagtcggcaa
     5461 aggccccgtc gcggtcaaga agcggcgtga gtccctgcac tacgtgcatg ggatgaacgt
     5521 cgcgcggcag cgagcggacc agccccgcct cgcgctcgga cagctccgct gaggccgcca
     5581 ggcgcgcgcc gaaatcggga tcgagccggc ccgtcgccac gagcgccgcg acctcgtcga
     5641 aaggcttgtc gaccagctcg tccacgtcga tgcctcggta gctcaggacc ccgcgctcac
     5701 cgtccaccag cgagatcccg gtctcgtcca cctccacgcc ttcgagccct ttgggcaggg
     5761 tgtcgctcga catgtcgttg gctcctttgt cattcgcgaa cggcccgcag cgtagcgcgg
     5821 cgatgatgtc ggcgaccatc cgctttcact tctccggaga tcaggccggc ttatccatta
     5881 gcccagataa tggctctata agtggagcgc ccccgcaggc cctggtagcc tccggacatg
     5941 cagatcgagc gccacgaaat caggatcttc agcgccgtgg tggaggaagg cggcttcagc
     6001 agggccgccg agcgtcttaa catcacccag tcggcggtca gccaggcggt ggcgaatctc
     6061 gagcacaagc tcgatgcccg gctgctcgaa cgccgcggca agccggagct cacggaggcg
     6121 ggcaagcggc tgttcgcgtt caccc
//
LOCUS       DBNHQR010000403         5566 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_227845, whole genome shotgun sequence.
ACCESSION   DBNHQR010000403 DBNHQR010000000
VERSION     DBNHQR010000403.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5566)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5566)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5566
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_227845"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="AC16VR_09120"
     CDS             <1..633
                     /locus_tag="AC16VR_09120"
                     /inference="COORDINATES: protein motif:HMM:NF018172.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030058 - aliphatic amine dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amine dehydrogenase large subunit"
                     /protein_id="HIR6011356.1"
                     /translation="ISTPGCALIYPTVERSFFMLCGDGRMLSITLDETGNPVSRKASE
                     PFIDIDADPLSEKSAKAGGIWHFVSYRGQVQPIDARAAQPELMTTWWLATEKERAANW
                     RPAGWHWTAGHKSGLLWVGMTPDGYDGSHKDPAPEIWLFDTGERERLARIELRAPAIS
                     ISVTQHEKPYLLVASTEATLDVYDAMSGEYLRSIYDLGFTPYMVHPVSYE"
     gene            645..1172
                     /locus_tag="AC16VR_09125"
     CDS             645..1172
                     /locus_tag="AC16VR_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010489016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0009308 - amine metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylamine dehydrogenase light chain"
                     /protein_id="HIR6011357.1"
                     /translation="MNWLDRITQKTTRKVARATSRRGFLAGASAVLLGASATPLLPVA
                     RASESRVPPQSTGNPEDPGDPTQCDYWRYCAIDGFLCSCCGGTQNACPPGTEMSPLTW
                     IGTCRNPVDGKNYVISYNDCCGKSSCGRCLCNRNEDDRPVYRPSANNDINWCMGTTSS
                     VYNCSTAVVLGLATE"
     gene            1176..1814
                     /locus_tag="AC16VR_09130"
     CDS             1176..1814
                     /locus_tag="AC16VR_09130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD domain-containing protein"
                     /protein_id="HIR6011358.1"
                     /translation="MLKLYALFGDDGAESPFQLNQAQALEVLQERFPELRRYRQFLRT
                     GEQLGNLVLAPFRASAEFWLAEHDDPTPLMAALETAAEDVHEVFAVNPRVLIRSEEHN
                     ISGEPFHDSGFKATFLFDRKPSLSLEEFHDYWLTHHGPIAAQTQDAERYVQSHIRTAQ
                     RAYDGVTELFWPDYPTALASMASEQMTTDQANDAPNFVDGESLVVFLAREAN"
     gene            complement(1875..2303)
                     /locus_tag="AC16VR_09135"
     CDS             complement(1875..2303)
                     /locus_tag="AC16VR_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6011359.1"
                     /translation="MKGRTVCFAFLIAAAPHAIGDGPVDAERARLNYMLNCQGCHLPD
                     GSGVEDAVPRMNGFVGNFLHVPGGREFLVRVPGSANAALDDASLAELLNWILVTMSPG
                     ELPPSWVPYSAGEVAELRSAPLREVEKVRAGLVRSIQQHL"
     gene            complement(2300..2821)
                     /locus_tag="AC16VR_09140"
     CDS             complement(2300..2821)
                     /locus_tag="AC16VR_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010489016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0009308 - amine metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylamine dehydrogenase light chain"
                     /protein_id="HIR6011360.1"
                     /translation="MNWLDKLSEKSTRGLAQQTSRRGWLAGIGTVLAGAASVPLLPMA
                     RASSGGQPKLEDVADTGDPLSCDYWRHCSIDGFLCGCCGGSHNTCPPGTEMSPITWVG
                     TCRNPVDGKNYVISYNDCCGKTPCGQCFCNTNQGDRPMDRPDKNNDINWCLGTESTVY
                     HCSTSVLIGLAVE"
     gene            complement(2841..3992)
                     /locus_tag="AC16VR_09145"
     CDS             complement(2841..3992)
                     /locus_tag="AC16VR_09145"
                     /inference="COORDINATES: protein motif:HMM:NF018172.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030058 - aliphatic amine dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amine dehydrogenase large subunit"
                     /protein_id="HIR6011361.1"
                     /translation="MRVFVVFSVLLAASGMSLAADQFEAEPLGEVAQLPSPNPDHWVV
                     IHDLSFNHMREGKLFLVDPHADSVSGQLRGMMSADFVASFLVSARRNEYYVIESFHAR
                     GGRGGERTDVVTIYDPATLSVLEEIIIPPKRLSGMPNRYQVALLDDDRYMVVFNFTPS
                     QSATVVDLERRQVLGEVAVPGCMFAYPTGKRGFSSLCSDGGMLTTRLGEDGSVAGTDR
                     LAPFNDVIDDAMFARPAEIDGVFYFPTFTGNVAPIDMRGEAAALGERWSLTTASERAQ
                     GWRPGGLWPAVGDGNGRLYMLMHGHGAEGTHKDGGDQVWIFDAANGKRIGKIELARWG
                     IALSVSTGERPQLMVSGAEPVIDVYEADGTHVRTLGVDMSTPLLVHGVR"
     gene            complement(4188..4757)
                     /locus_tag="AC16VR_09150"
     CDS             complement(4188..4757)
                     /locus_tag="AC16VR_09150"
                     /inference="COORDINATES: protein motif:HMM:NF014534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HIR6011362.1"
                     /translation="MQIPLQITWRNIPKSEALEADIVAKAEKLEEFFDHILSCRVVVE
                     KSHGRHRKGNLYRLRLDIHVPDKEIVVTRDPGDDHAHEDMYVSIRDAFDAARRQLQDY
                     VRVRRGHVKPHDQHHVGRVLRRIAAEDYGFLGTADGREIYFHRNSVVNADFDKLEPGT
                     EVMFVEETMNEGVQAKMVSVGKHRFPEGS"
     gene            complement(4903..5301)
                     /locus_tag="AC16VR_09155"
     CDS             complement(4903..5301)
                     /locus_tag="AC16VR_09155"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR6011363.1"
                     /translation="MDIQDRIDRDGGVLERLNISVDLSANAGVRLEMKVAEDMVNAGG
                     YCHGGLLFALADTACAYALAEAGLAPVTLDAGITYLRPATAGERIAAETEVVRAGRRI
                     GHCEVRLTREDGTLLAIYRGTCSNASSDSR"
     gene            5378..>5566
                     /locus_tag="AC16VR_09160"
     CDS             5378..>5566
                     /locus_tag="AC16VR_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007162719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR6011364.1"
                     /translation="MGVRCIKCPLADPQDTTVTATHHRLIVLGSGPAGYTATLYAARA
                     NLRPALITGVEVGGQLTTT"
BASE COUNT         1044 a         1878 c         1700 g          944 t
ORIGIN      
        1 atcagcacgc ccggctgcgc gctgatctat ccgaccgtcg agcgcagctt cttcatgctc
       61 tgcggagacg ggagaatgct ctcgatcacg ctggatgaga ccggtaatcc cgtttccaga
      121 aaggccagcg agccgttcat cgacatcgac gcggaccccc tgtccgagaa gtccgccaag
      181 gccggcggaa tctggcattt cgtcagctat cgcgggcagg ttcagcccat cgatgcccgt
      241 gccgcgcagc ccgagctgat gacgacctgg tggctggcca cggagaagga acgtgccgcc
      301 aactggcgtc cggccggctg gcactggacg gcaggccaca aaagcggatt gctctgggtg
      361 ggcatgacgc ccgacggcta cgacggcagc cacaaggacc cggcccccga aatctggctc
      421 ttcgacaccg gcgagcgcga gcgcctggcc cgcatcgagc ttcgcgcgcc cgccatttcc
      481 atcagcgtca cccagcacga gaaaccctat cttctggtgg caagcaccga ggccaccctg
      541 gacgtttacg acgccatgag cggcgaatac ctgcgatcaa tctacgatct cggtttcacg
      601 ccttacatgg tccaccctgt gagctacgag taaggagccg cgcgatgaac tggctggacc
      661 ggatcaccca gaaaaccacg cgcaaggtcg cacgagccac ttcgcggcgg ggctttctcg
      721 ccggcgcctc agcggtcctg ctgggcgcat cggcaacccc gttgctgccc gtagcacggg
      781 ccagcgagag ccgcgtgccc ccccaaagca cgggcaaccc cgaggatccg ggagatccca
      841 cccagtgcga ctactggcgc tactgcgcca tcgacgggtt cctgtgcagc tgctgcggcg
      901 gcacccagaa cgcctgcccg ccgggcacgg agatgtcgcc tctcacctgg atcggcacct
      961 gccgcaatcc ggtggacggc aagaactacg tgatctccta caacgactgc tgcggcaaga
     1021 gcagctgtgg acgctgcctg tgcaaccgta acgaggacga ccggcccgtc taccgaccga
     1081 gcgccaacaa cgacatcaac tggtgcatgg gcacgacctc gagcgtgtac aactgctcca
     1141 ccgccgtggt gctcggcctg gccaccgagt agatcgtgct caagctctac gccctgttcg
     1201 gcgacgacgg ggccgaatca ccctttcagc tgaatcaagc gcaggcactc gaggttcttc
     1261 aggagcgttt tccagagctg cgtcgctatc gccagtttct gaggacgggg gagcaactcg
     1321 gcaatctggt gctcgccccc ttccgcgcct cggccgagtt ctggctggca gagcacgacg
     1381 acccgacacc gctgatggcg gcgctcgaga cggccgctga ggacgttcac gaggtcttcg
     1441 ccgtcaaccc ccgtgtgctg atccgcagcg aagagcacaa catcagcggc gagccctttc
     1501 atgacagcgg ctttaaggcg accttcctgt tcgatcggaa gccgtcgctc agcctggagg
     1561 agttccacga ctactggctg acccatcacg gccccatcgc cgcccagacc caggacgcgg
     1621 agcgctacgt gcagagccac atcaggaccg cccagcgggc ctacgacggc gtcacggagc
     1681 tgttctggcc cgactacccg acggcgctcg ccagcatggc ttcagagcag atgaccaccg
     1741 accaggccaa cgacgctcct aacttcgtgg atggcgagag cctggtcgtg ttcctggccc
     1801 gcgaggcgaa ctgagaccga cgagttttcg gccgccatct tccccttggg tcaggcttgc
     1861 cagaacctcc aaggtcagag gtgctgctgg atgcttcgga ccagcccggc cctcaccttc
     1921 tccacttctc tgagcggtgc cgaacgcagc tcggccacct cgcccgccga gtagggcacc
     1981 cagctcgggg gaagctcgcc ggggctcatg gtgacgagaa tccagttgag cagctcggcc
     2041 agggaggcgt cgtccagcgc cgcgtttgcg gatcccggca cgcggaccag gaactctctg
     2101 cctcccggga catgcaggaa gttgccgacg aagccattca tccgcggcac cgcgtcttcc
     2161 accccggaac catcggggag atggcatccc tggcagttca gcatgtagtt caggcgtgct
     2221 cgctccgcgt cgaccggccc gtccccgatg gcgtgaggcg cggcggcgat cagaaaagca
     2281 aagcagaccg ttcgcccctt cactcgacgg caagccctat cagcacggac gtggagcagt
     2341 gatagacggt gctttcggtg ccaagacacc agttgatgtc gttgttctta tcgggccggt
     2401 ccatgggccg gtcgccctga ttggtgttgc agaagcactg tccgcagggc gtttttccgc
     2461 agcagtcgtt gtaggagatc acgtagttct tgccgtcaac gggattgcgg caggtaccca
     2521 cccaggtgat gggcgacatc tccgtgcccg gcgggcaggt gttgtgagag ccgccacagc
     2581 agccgcagag aaaaccgtcg atggagcaat gccgccagta atcacaggag agcgggtcgc
     2641 cggtgtcagc aacgtcttcc agcttcggct gcccgccact ggaagccctt gccatcggca
     2701 gcaggggaac cgacgcggcg ccagccagca cggtgccgat acccgcaagc cacccgcggc
     2761 gggacgtctg ctgggcgagc ccgcgcgtag acttctcaga tagtttgtct aaccaattca
     2821 tcgacgatct ccaggtctgt tcagcgaacg ccgtgcacga ggagcggcgt ggacatgtcc
     2881 acgccaaggg ttctcacatg ggtgccgtca gcctcgtaga cgtctatgac cggctcggcg
     2941 ccgcttacca tcagctgagg ccgctcgccg gtggagacgc tcagcgcgat gccccagcgg
     3001 gccagctcga tcttgccgat gcgcttgccg ttggccgcat cgaatatcca cacctgatcg
     3061 ccgccgtcct tgtgagtacc ctccgcgccg tgtccgtgca tcagcatata gagccggccg
     3121 ttgccgtcgc cgacggcggg ccagaggccg ccgggacgcc agccctgggc acgctcggag
     3181 gccgtggtca gagaccaccg ttctcccagc gcggccgctt cgccgcgcat atcgatggga
     3241 gccacgttgc ccgtaaacgt cggaaagtaa aacacgccgt cgatctctgc cggccgggcg
     3301 aacatggcat cgtcgatgac gtcgttgaat ggcgccagcc gatccgttcc cgcgaccgat
     3361 ccgtcttcgc caagccgggt cgtcagcatc cccccgtcgc tgcacagcga gctgaacccg
     3421 cgtttgccgg tcgggtaggc aaacatgcat ccgggaacag ctacctcccc tagcacctgc
     3481 cgacgctcga gatccaccac ggtggcagac tgcgacggcg tgaagttgaa gacgaccatg
     3541 taccgatcgt cgtcgagcag ggccacctga taacggttgg gcatgcctga caggcgcttc
     3601 ggcggaatga tgatttcttc cagcaccgac agggtggctg gatcgtagat ggtgacgacg
     3661 tcggtgcgtt cgccgccccg acctccgcgg gcgtggaagc tctcgatcac gtaatactcg
     3721 ttgcgccggg cgctgacgag gaagctggcg acgaaatcgg cggacatcat gccgcgcagc
     3781 tggccggaca ccgagtccgc atggggatcg acgagaaaga gcttgccctc ccgcatgtga
     3841 ttgaaggaca gatcgtgaat caccacccag tgatccggat tgggggacgg cagctgggca
     3901 acctcaccca gaggctcggc ctcgaactgg tcggccgcaa ggctcatgcc ggatgcagcg
     3961 agcagcacgc tgaagacgac gaaaacccgc atggattcct cccactgatc cctggccgaa
     4021 gtgtaacccg cgcgttggcg atcaccaacc aatcggcact acctgcttcc aatcgacagg
     4081 gcggagacag aaattcacga ccccacccat tgccgatcac gcggcgggac ggcactctgc
     4141 cggcggggat ggcggcggct gagccgtccg gcattccgcc accgagatca ggaaccttcg
     4201 ggaaatcggt gcttgcccac ggaaaccatc ttcgcctgaa cgccttcgtt catggtttcc
     4261 tccacgaaca tcacctcggt accgggctcg agcttgtcga agtccgcatt gaccaccgag
     4321 ttacgatgaa agtagatctc gcgaccgtcg gccgttccga gaaagccgta gtcctccgcg
     4381 gcgatccttc gcagaacccg gccaacatga tgctgatcgt gaggcttgac gtgtccgcgc
     4441 ctgacgcgga cgtagtcctg gagctgccgc cgagcggcgt cgaaggcatc gcggatgctc
     4501 acgtacatgt cttcatgggc gtgatcgtcc cccggatcac gcgtcaccac gatctctttg
     4561 tccgggacgt ggatgtcgag ccgcaggcgg tagagattac ccttccggtg ccgcccatga
     4621 gacttctcca ccaccacacg gcagctcaag atgtggtcga aaaactcttc gagcttctcc
     4681 gccttcgcga cgatgtcggc ctccagggct tcgcttttgg ggatgtttcg ccaggtaatc
     4741 tgcaagggta tctgcaagtc gcaactccca tcacgttcta cacacgtcca aagatgcgcc
     4801 tttgggttcc gattggcgct ccggatttcc acgtagttcc ccgcgggggc cggtaggcgt
     4861 gcagccgaac ccggcacgcc ccgcgaaccc cggctgccgg ggttagcgag aatccgagga
     4921 agcattggag caggtccccc ggtagatggc cagcagggtt ccgtcctcgc gcgtgagccg
     4981 gacctcgcaa tgtccgatcc gccgccccgc gcgaacgact tcggtttcgg cggcaatccg
     5041 ttcacccgcg gtcgccgggc gaaggtaggt tatgcccgca tcgagtgtca ccggcgccaa
     5101 tcctgcctcg gccagggcat aggcgcacgc ggtgtccgcc agcgcgaaga gcagaccccc
     5161 gtgacagtat ccacccgcgt tgaccatgtc ttccgccact ttcatctcga gcctgacgcc
     5221 ggcgttcgcc gacagatcga cgctgatgtt caaccgctcg aggacaccgc cgtcgcggtc
     5281 gatgcgatcc tgaatatcca agctggcccc gcccgcgaat caataggccc aataaacctc
     5341 caataccgag ccggagtcaa cgcgtgagcg ttcacccatg ggcgtgcgct gtataaaatg
     5401 cccgctcgct gatccacagg acacaaccgt gaccgccaca catcatcgac tcatcgtgct
     5461 cggatcaggg ccggccggat acaccgcaac gctgtacgcg gcccgggcga acctgcgacc
     5521 cgcgctgatt accggggtgg aggtcggcgg gcagctgacc acgacg
//
LOCUS       DBNHQR010000404         3613 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_481055, whole genome shotgun sequence.
ACCESSION   DBNHQR010000404 DBNHQR010000000
VERSION     DBNHQR010000404.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_481055"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="AC16VR_09165"
     CDS             complement(<1..442)
                     /locus_tag="AC16VR_09165"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6011365.1"
                     /translation="MSWEDIPILVVDDAKYSSAVITKALTDEGFSNVRCVNDGFAALD
                     TLENRSAQILIADWVMPAMDGLELTRRVKRLDEENGHFTYVMLLTGREDFEGMLEAFD
                     TGVDDFLNKHNLRAELQPRIIAAQRIAARRNELLRTNKLLRKKVR"
     gene            651..1133
                     /locus_tag="AC16VR_09170"
     CDS             651..1133
                     /locus_tag="AC16VR_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HIR6011366.1"
                     /translation="MPSFDVISEVDLQEVRNAVDQASRELRTRFDFKGVDASFEYTDE
                     GILLAAMEEFQLGQMLDILKDKLNRRGVDVRALDPGTIEASGKQKRQAFALKQGIDRD
                     TAKRLVKILKDSRLKVQSQIQGEQVRVTGKKRDDLQQAIAALKDAGLEVPLDFTNFRD
                     "
     gene            1189..2412
                     /locus_tag="AC16VR_09175"
     CDS             1189..2412
                     /locus_tag="AC16VR_09175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HIR6011367.1"
                     /translation="MITCAFTGFASGLPLFVLVQLVQAWLRDEGVGLTEIGLFALAQV
                     PYTWKFIWSPLAERYPLPFLGRRRGWMLVTQVALVAGFAGFGYVDPVLNIEAVFALAL
                     LVGFFSASQDIVIDAYRRELLPTEEELALGNSIHVQVYRIAGFVPGSLGLILADVLPW
                     SAVYWTMAGFMAIGVATTLVVREAVLDPRIPPSLKAAVVEPFREYFGRRGWRYGLVVL
                     LFLFAYKFGDSMATALSTTFYLDLGFSKTEIGVVAKNAGFWPALIGGLLGGLFIVKIG
                     INRGLWVFGVVQLITILGFAYLAQVGNDLVVLAVVISAEYLGVGLGTAAFVAFIARET
                     SPALAATQFALFSAVTALPRTLTAAATGWLVDVVGWVEFFYLCTLAAVPGMVLLVWVA
                     PFTAPSNEPQPPGTR"
     gene            2555..3010
                     /locus_tag="AC16VR_09180"
     CDS             2555..3010
                     /locus_tag="AC16VR_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HIR6011368.1"
                     /translation="MGLWFLVFFLTPILEMYLLIEVGGYIGTLPTIALVMLTAVVGVA
                     LLRIQGVETLTRGVGRLQRGEVPATEMAEGILLAVAGALLITPGFVTDAIGFVLLFPP
                     SRTGIARRMLSRLTMVGPDGRPFSPGEPGPGPTVIEGEFERRPPDDGQP"
     gene            3140..3430
                     /locus_tag="AC16VR_09185"
     CDS             3140..3430
                     /locus_tag="AC16VR_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020799908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HIR6011369.1"
                     /translation="MNIRPLHDRVVVRRLEEESTSAGGIVLPDSAAEKPSQGEVLAIG
                     SGKLLDNGDVRALDVKVGDRVLFGQYAGSTVKIDGEELLVLSESEIFGVLEG"
     gene            3457..>3613
                     /gene="groEL"
                     /locus_tag="AC16VR_09190"
     CDS             3457..>3613
                     /gene="groEL"
                     /locus_tag="AC16VR_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253075.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; many bacteria have multiple
                     copies of the groEL gene which are active under different
                     environmental conditions; the B.japonicum protein in this
                     cluster is expressed constitutively; in Rhodobacter,
                     Corynebacterium and Rhizobium this protein is essential
                     for growth; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIR6011370.1"
                     /translation="MSAKDVVFGDEARSRMLEGVNVLAEAVKVTLGPKGRNVVLDKSF
                     GAPTLTND"
BASE COUNT          592 a         1089 c         1186 g          746 t
ORIGIN      
        1 cgcgtacctt ctttcgcagg agcttgttgg tgcgcagcag ctcgttccgg cgcgcggcga
       61 tgcgctgggc ggctatgatt ctgggctgca gctcggcgcg caggttgtgc ttgttgagaa
      121 agtcgtccac cccggtgtcg aaagcttcca gcatgccttc gaaatcttcc ctccccgtga
      181 gcagcatcac gtacgtgaag tggccgttct cctcgtccag gcgtttcacc cggcgggtca
      241 gctccagccc gtccatggca ggcatcaccc agtcggcgat gaggatctgg gccgaacggt
      301 tttccagcgt gtccaacgcg gcgaagccgt cgttcacgca gcgcacgttg ctgaaccctt
      361 cgtcggtcaa agctttggtg atgacggcgc tggaatactt cgcgtcatcg acgacgagga
      421 ttggaatgtc ttcccatgac atgtaagtta gcgtttgctt cgacgccttg tcgcttcctt
      481 gatatgcgag ggacccctcg atgtcaagga aggttcatcg ctcgtgcggc catggtctca
      541 ggatggtgat ctggttcacc tttctgcgcc gcgtggggcc ggcgcctgca gcgcgaaacc
      601 aggcgcctgg ggcgcgatgc tagatgctac tagatgccaa aggaaacgct atgccaagct
      661 tcgacgtgat ttccgaggtg gatctgcagg aggttcgcaa cgcggtggat caggcatcgc
      721 gggagcttcg cacccgattc gatttcaaag gcgtcgacgc cagcttcgag tatacggacg
      781 aggggatcct tctggctgcc atggaggagt tccagctggg tcagatgctg gacatcctca
      841 aggacaagct gaaccgccgc ggcgtggacg tccgtgctct cgatcccggc acgatcgagg
      901 cgtccggcaa gcagaagcgg caggccttcg ctctcaagca gggtatcgac cgcgatacgg
      961 ccaagcgcct ggtcaagatc ctgaaagact ccaggctaaa ggtccagagc cagattcagg
     1021 gcgagcaggt gcgcgtgacg gggaagaaac gggacgatct tcagcaggcc atcgccgccc
     1081 tgaaggacgc aggcctggag gtgccgctcg acttcaccaa cttccgcgac tgacgcagcc
     1141 cctgccggac ggcccgcctt gaaactccgt gaggtactca accggcgcat gatcacatgc
     1201 gcgttcacgg gtttcgcatc gggcctgccg ctgttcgttc tggtgcagct ggtgcaggcg
     1261 tggctgcgcg acgagggcgt cgggctgacg gagatcgggc tgttcgcgct cgctcaggtt
     1321 ccgtacacgt ggaagttcat ctggtcgccg ctggcggaac gctacccgct gcccttcctg
     1381 gggcgccggc ggggctggat gctggttacc caggtcgcgc tggttgccgg ctttgcgggg
     1441 ttcggctacg tggatcccgt gctgaacatc gaagccgtgt tcgccctggc gctgctggtc
     1501 ggttttttca gcgccagtca ggacatcgtc atcgacgcct ataggcgcga gctgctgcct
     1561 accgaagaag agcttgcgct cggcaactcc attcacgttc aggtataccg gattgccggt
     1621 ttcgtgccgg gctccctcgg cctgatcctc gccgacgtgc tgccctggag cgccgtgtac
     1681 tggaccatgg ccggcttcat ggccatcggc gtggcgacca ctttagtggt gcgcgaggcc
     1741 gtgctggacc cgcggatacc gccatcgctg aaagccgccg tggtggagcc ttttcgggaa
     1801 tatttcggcc gccgcggatg gcgctacggg ctggtggttc tgctgttcct gtttgcctac
     1861 aagttcggcg acagcatggc gaccgccctg tcgacgacct tttacctcga tcttggattc
     1921 agcaagacgg aaatcggcgt ggtggccaag aacgccggat tctggcccgc gctcatcggc
     1981 ggcctgctgg gcggtctgtt catcgtcaag atcgggatca accggggctt gtgggtgttc
     2041 ggcgtggtgc agctgatcac catcctgggc ttcgcctatc tggcccaggt gggcaatgac
     2101 cttgtggtgc tcgcggtggt gatctccgcc gagtacctgg gggtagggct cgggacggcc
     2161 gccttcgtgg ctttcatcgc ccgtgagact tcgcccgctc tggcggccac ccagtttgcc
     2221 ctgttcagcg cggtaaccgc gctgccgcga acgctgaccg ccgcggcgac cggctggctg
     2281 gtggatgtgg tcggctgggt cgagttcttc tatctgtgca ccctggcagc ggttccgggg
     2341 atggtgctgc tggtgtgggt cgcgcccttc acggctccca gcaatgagcc gcagccaccc
     2401 gggacccgct gaacgagacc cgctcggcct tcaggcgtgc catctggcct gcgggatcat
     2461 cgccaccacc tgccacaccc gctgcctcgg tgacggttga ctatccgcga gcgtgccctc
     2521 agattgagcc gacctcgtct ggtcacccgc catcatggga ttgtggttcc tcgtcttctt
     2581 cctcaccccc atcctggaaa tgtacctgct catcgaggtg ggcggctaca tcgggacgct
     2641 gccgaccatc gctctggtca tgctgactgc ggtggtcggc gttgcgctgc tgcgcattca
     2701 gggagtagag acgctgaccc ggggggttgg tcggctgcaa cgcggcgagg tgcctgcgac
     2761 ggagatggca gaaggaattc ttctggccgt ggcgggggcc ctgctcatca ccccgggttt
     2821 cgtgaccgat gccatcgggt tcgttctgct cttcccgccg agccgcacgg gtatcgcacg
     2881 gcgcatgctc tccaggctca ccatggtcgg gcccgacggt cgccctttct cgcccggaga
     2941 gccgggtccc gggcccacgg tcatcgaggg agagttcgag cggcgcccgc ccgacgacgg
     3001 ccagccgtaa aacgtttact tcccaggact tgaatctctc cgggccgcca ctattatatg
     3061 cggcccttgg attagcactc tgagcgttag agtgctaacg attacccgat agggacttta
     3121 actgggagaa acacaggtaa tgaacattcg acccttacac gatcgcgttg tcgtgcgccg
     3181 gttggaagag gaaagcacct cggcaggcgg catcgtgctg cccgactctg cggcagagaa
     3241 gccttctcag ggcgaggtgc tggccatagg ttccggcaag ttgcttgata acggtgacgt
     3301 ccgtgcgttg gacgtcaagg taggcgaccg agttcttttc ggccagtacg ccggcagcac
     3361 ggtgaagatc gacggtgagg aactgcttgt cctcagcgag agcgagatct tcggcgtcct
     3421 ggaaggctaa ccagcgagat ttatgaggaa aaagcgatga gcgctaaaga cgtagttttt
     3481 ggtgatgaag cccggagccg gatgctcgag ggcgtgaatg ttctggccga agcggtcaaa
     3541 gtcacgctgg gcccgaaggg gcgcaacgtg gtgctggaca agtcgttcgg cgcgccgacc
     3601 ctgaccaacg acg
//
LOCUS       DBNHQR010000405         2680 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_608300, whole genome shotgun sequence.
ACCESSION   DBNHQR010000405 DBNHQR010000000
VERSION     DBNHQR010000405.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_608300"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="AC16VR_09195"
     CDS             <1..354
                     /locus_tag="AC16VR_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6011371.1"
                     /translation="VMVVDDSVTVRKVTSRLLERQGMDVLVAKDGIEAIAMLQERRPD
                     VMLLDIEMPRMDGFEVARQVRRDERVQDLPIVMISSRTGEKHQEHAAELGVNRFLGKP
                     FQENELLATIDSLVN"
     gene            365..856
                     /locus_tag="AC16VR_09200"
     CDS             365..856
                     /locus_tag="AC16VR_09200"
                     /inference="COORDINATES: protein motif:HMM:NF013731.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HIR6011372.1"
                     /translation="MEPATAQELIEMSCVMIPLQETNLLLPNICVAEILPWRRVSSIE
                     DAPKWCLGMLEWRGEVIPVVRFERMGDASVEDAEEGRCLIVMNRSRTIEGLPFYALAA
                     EGLPRIVQLTAEDMANVPTNLGVSETMALRVGSEPAIIPNLQYLEVQVANLVRGQETA
                     KQA"
     gene            complement(853..1572)
                     /locus_tag="AC16VR_09205"
     CDS             complement(853..1572)
                     /locus_tag="AC16VR_09205"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HIR6011373.1"
                     /translation="MSRRIYLEASLLPGATVRLDPDHSHYLTRVMRLKAGDALRCFDG
                     SGREHAARLAEPNPRSCSIEIAEELRNAAPATVRLHLAQAWLKGQAMDLVIQKATELG
                     VTDIWPIEALRSNVRIDAGRTAGRLMHWRGIAKSATEQSERLFLPRVHEPRRLEALLT
                     ERPCQRLILLDPGAAALPGALPFDSLVLMVGPEGGWTEHERELAQAAGADRYGLGDLV
                     LRAETAPIAALAAVRQSWGWR"
     gene            complement(1569..2426)
                     /locus_tag="AC16VR_09210"
     CDS             complement(1569..2426)
                     /locus_tag="AC16VR_09210"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - L-methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HIR6011374.1"
                     /translation="MPERGQRPRLSFEFFPPRSEEGFASLARVTAKLDELRPDFYSCT
                     YGAGGSTRDGTKQTVARLVSSGHATAPHLSIGADEEETTFAMLDGYRGMGIDRIVALR
                     GDVPSGIGRSRFRANAQALVGRIRKHSGTHFHIEVAAYPEVHPDARSPGSDLEFFRRK
                     VDAGADSAITQYFYSPHAYYDFVDRCAGAGVTIPIYPGIMPITSYEGLIRFSDNCGAD
                     VPRWLRKHLEAFGDDDDGLRRFGIETVSRLCDDLLRAGAPGLHFYTLNRWGASRAICQ
                     NLGLANRDG"
     gene            complement(2452..>2680)
                     /locus_tag="AC16VR_09215"
     CDS             complement(2452..>2680)
                     /locus_tag="AC16VR_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007021908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HIR6011375.1"
                     /translation="TSISINTFGTGKLDDERIIQLVREHFDLRPKGLIDMLDLKRPIY
                     RNTAAYGHFGRSEPEFTWERTDKAEALAAAL"
BASE COUNT          463 a          846 c          904 g          467 t
ORIGIN      
        1 gtcatggtgg tggacgactc ggtcacggtt cgcaaggtga cctcccggct gctggagcgg
       61 cagggcatgg acgtcctcgt ggccaaggac ggcatcgaag ccattgccat gctgcaggaa
      121 cgccgcccgg acgtgatgtt gctcgatatc gagatgccgc gcatggacgg cttcgaggtg
      181 gctcgccagg tgcggcgcga cgagcgcgtg caggacctgc cgattgtcat gatcagctcg
      241 cgaacgggcg agaagcacca ggagcatgcc gccgagctcg gggtgaaccg cttcctcggc
      301 aagccgttcc aggagaacga gctgctcgcc accatcgact cgctggtgaa ctaaagagaa
      361 gcccatggaa cctgctaccg cacaagaact catcgaaatg tcctgcgtga tgatcccgct
      421 gcaggagacg aatctgctgc tacccaacat ctgcgtagcg gagattctgc cctggcgccg
      481 ggtcagcagc atcgaggacg cgccgaagtg gtgtctcggg atgctggagt ggcgtggcga
      541 ggtcatcccc gtggttcgtt tcgagcgaat gggcgacgcg tcggtggaag atgcggaaga
      601 gggtcgctgc ctcatcgtga tgaaccgctc tcgcaccatc gaggggctgc cgttttacgc
      661 cctggcggcg gagggtctgc cgcgcatcgt gcagctcacc gccgaggaca tggcgaacgt
      721 cccaaccaat cttggtgtgt ccgagaccat ggccctcagg gttggcagcg agccggccat
      781 catccccaac ctgcagtatc tcgaggttca ggtggccaac ctggtgcgag gccaggaaac
      841 cgccaagcag gcttagcgcc agccccagga ctgtcggacc gcagcgaggg cggcgatggg
      901 cgcggtttcg gcccggagca caagatcccc caagccgtag cgatcggctc ccgccgcttg
      961 agccagctcc cgttcgtgct cggtccaccc cccctcgggt ccgaccatca ggaccagcga
     1021 gtcgaaaggc agcgcgccgg gaagagccgc tgcccctgga tcgagcagga tgagccgctg
     1081 gcaggggcgt tcggtcaaaa gcgcttcgag ccgtcggggc tcgtgcaccc gcggcaggaa
     1141 cagccgctcg ctctgctccg ttgcgctctt ggcgattccc cgccagtgca tcaggcggcc
     1201 agccgtgcgc cccgcgtcga ttctgacgtt gctgcgcagg gcctcgatgg gccagatgtc
     1261 cgtgaccccg agctcggttg ctttctgaat cacgagatcc atggcctggc ctttcagcca
     1321 tgcctgggcc aggtgcagcc ggacagttgc cggcgcggcg ttgcgcagct cctcggcgat
     1381 ctcaatcgag caggagcggg ggttgggttc ggccagccgg gcagcatgct cgcgcccgct
     1441 gccgtcaaag catcgcagag cgtccccggc cttcaggcgc atcacccgcg tgaggtagtg
     1501 ggaatggtca ggatcgaggc gaacggttgc accgggcagc agcgacgcct ccaggtagat
     1561 gcgccgactc atccgtcccg gttagccaag cccaggttct gacagatcgc ccggctcgcg
     1621 ccccatcggt tgagggtata gaaatgcaga ccgggagcgc ctgcgcgcag caggtcgtcg
     1681 cacaggcggc tgacggtttc gatgccgaac cggcgcaggc cgtcgtcgtc atcgccgaag
     1741 gcttccagat gcttgcgcag ccagcggggc acgtccgcgc cgcagttgtc ggagaaacga
     1801 atcaggccct cgtagctggt aatgggcatg attcccgggt agatgggtat ggtcaccccc
     1861 gcccccgcgc agcggtcgac aaagtcgtaa taggcgtgcg ggctgtagaa gtactgcgtg
     1921 atcgcgctgt cggccccggc gtctactttg cgccggaaga attccaggtc ggagccgggg
     1981 gatctggcgt cgggatggac ctcgggatag gcggccacct cgatatggaa gtgggtgccg
     2041 gaatgcttcc ggatccggcc caccagggct tgcgcgtttg cgcgaaagcg cgagcgtccg
     2101 atgcccgaag gaacatcccc acgcaacgcc acgatacggt cgatgcccat gccgcgatac
     2161 ccatcgagca tggcgaacgt ggtctcctcc tcgtcggccc cgatggacag gtgcggcgcg
     2221 gtggcgtgcc cgctgctcac gagccgcgcg accgtctgct tggtgccgtc ccgggtcgat
     2281 ccgccggcgc cgtacgtgca ggaataaaaa tctggccgca actcgtcgag tttagccgtc
     2341 acccgggcga ggcttgcgaa accctcctcg ctgcggggcg ggaagaactc gaagctgagc
     2401 cttggccgct ggcctcgttc gggcatgctg acgcgttact ccgggttgac gttacagcgc
     2461 tgccgccagt gcctccgcct tgtcggtgcg ctcccaggtg aattcgggct cagatctgcc
     2521 gaaatgaccg tatgcggccg tgttccgata gatcgggcgc ttcagatcca gcatgtcgat
     2581 gaggcctttc gggcgcaaat caaaatgctc acgtacgagt tgaatgatcc tctcgtcgtc
     2641 gagcttgccc gtgccgaaag tgttgatgct gatggacgtg
//
LOCUS       DBNHQR010000406         4439 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_417845, whole genome shotgun sequence.
ACCESSION   DBNHQR010000406 DBNHQR010000000
VERSION     DBNHQR010000406.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_417845"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="AC16VR_09220"
     CDS             <1..383
                     /locus_tag="AC16VR_09220"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RHO alpha subunit C-terminal catalytic
                     domain-containing protein"
                     /protein_id="HIR6011376.1"
                     /translation="MRNSNVSYLMFPNHLTPGGARADGTIGMTLQSFWPISPNQTLSE
                     WHIMVPDWGDGPPPANSERVVDGYSVIMHEDTGYLEHVQKSIESGAIDGLLTSYHERR
                     VYHHEASIDRLIGIDNVPEHLRVE"
     gene            423..1025
                     /locus_tag="AC16VR_09225"
     CDS             423..1025
                     /locus_tag="AC16VR_09225"
                     /inference="COORDINATES: protein motif:HMM:NF037008.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6011377.1"
                     /translation="MALPAESNPQEEIIAATERLLAEDGPQKATVRAITQAAGVNVGA
                     VNYHFGSRDGLMMAICARHMGDSNQDMIDRLQSLAIAEGRESVRRIFEPIVQTAFSVW
                     LRDDVLRGLRNFMFVDRNLVHKLDVSEMSQVYECMQDALREACPSLSAREVRRRFRFA
                     MGTIMHEVRSHDDRRTAEAGEAVVEDLLEFVAGGFAYGQV"
     gene            1012..2013
                     /locus_tag="AC16VR_09230"
     CDS             1012..2013
                     /locus_tag="AC16VR_09230"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6011378.1"
                     /translation="MVRFELRYALRQPEFKGDDFQPRYQACIEQCAWGDRLGFSAVMI
                     SEHHGSPDGYMPSPMVLGGAIAAVTEHMRIRILSLVGPLHNPVRLAEDLATLDLVSNG
                     RVDPCISGGYVGYEFEAMGTSLTCRKEYMEEIVPFLHKAWTGEPFEWKGRTIRVTPRP
                     VQRPGPPIWMGGASKASARRAARHADVFLPAHPDLMKTYREELGKLGRPPKEPRGRSM
                     LVWLAEDPDRFWAEFGPSALHENNAYGQWYEDWGAWNGYVKEESTDALRATERYPVMT
                     PDELVARIEERGGNMTVMFHPMAGGAEPELAWQSLQLIEDKVIPALRDKGIETGGSQ"
     gene            2010..3749
                     /locus_tag="AC16VR_09235"
     CDS             2010..3749
                     /locus_tag="AC16VR_09235"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl-D-amino-acid deacylase family protein"
                     /protein_id="HIR6011379.1"
                     /translation="MSDYDILVRGGTVIDGTMIPRYRADVGIKDGRVARIGVGRKATA
                     ERVIDAGDNIVAPGYIDIHTHYDAQILWDPYCTISGWHGVTSVVLGNCGFGFAPVPPH
                     LRERAMLMMTRNEAIGFETMKEGMEWDRYGWETMPEWIEHLKRNVRLGVNATALVPLN
                     PLYAYVMGGIDEAKERRPTAAEKEEMIRLMYEALDHGACGFSYQRCGVPSVQPDWDGS
                     PMITDMLPDAEIIEFGRALGDYGRGFIEMFDAAPSDHDTVEAFMTSLARASGRPIVRN
                     ILLANDEYPKAHRAFLEWLNEAHEEGLQVFGMGFTVRSPTVLTFEDWSLWDGFPGWNR
                     VMNGPKEQRAELMRNESMRVQLRKEMDTGRVGGLGTSGKPAKLIINDTAGYGELDHYL
                     GRMVSEIAETENKHIVDVILDAVLASDFKVEFRGNAYDTSVETTMEVLNSPYVVPGIS
                     DGGAHTHFSVQGAYPTDLLEWLVREEGVMTAERAHYGLSRLPAHMCDLGDRGIIREGA
                     PADLVIYNPETIRRTPSWDTLDKLRDLPANEWRLVQRAEGLNYTLVNGVVTFEGLECT
                     GATPGELMLHGEV"
     gene            3753..4298
                     /locus_tag="AC16VR_09240"
     CDS             3753..4298
                     /locus_tag="AC16VR_09240"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6011380.1"
                     /translation="MATDAGHGGAAMEAQADEIGRLREEVARLGARLQHLEAVQAVRE
                     TLLDYAHYHDRGLSGELAELFAEDAVLDISGFGDDLDVSISGRGAIRDMYASLDARSD
                     GPPPYKHAITNLRIEIDGEQALASSYLLDWGGPATDLGPGGSVYRQRLERQSDGRWLI
                     THKRIICTAELTVEAASSLPR"
BASE COUNT          839 a         1330 c         1514 g          756 t
ORIGIN      
        1 tcatgcgcaa cagcaacgtc tcctatctga tgttccccaa tcatctgacc ccgggtgggg
       61 cccgggcgga cggcaccatc ggtatgacgc ttcagagttt ctggccgatc tctcccaatc
      121 agaccctgtc ggaatggcac atcatggtgc cggattgggg ggacggaccg ccgcccgcca
      181 actccgagcg ggtggtggac ggctactcgg tgatcatgca cgaggacacg gggtatctcg
      241 agcacgtgca gaaatccatc gagtccgggg ccatcgacgg cctgctcacc agctaccacg
      301 aacggcgggt gtaccaccac gaagccagca tcgatcggct gatcggcatc gacaacgttc
      361 ccgagcacct gcgggtggag tagatattgc ccatcgcgga tgctgattag gatcggcgcg
      421 aaatggcact acctgcagaa agcaatccgc aagaagagat catcgcggct actgagaggc
      481 tgctggccga agacggcccg caaaaggcca cggtgcgcgc gatcacccag gcagcgggtg
      541 tgaacgtagg ggccgtcaac taccacttcg ggtcgcggga cggtctgatg atggccatct
      601 gcgcccggca catgggcgat tccaatcagg acatgatcga tcgtctgcag agccttgcca
      661 ttgccgaagg gcgtgagagc gtaaggcgca tattcgaacc catcgtgcag acggcttttt
      721 ccgtctggct gcgcgacgac gtgctcaggg ggctgcgcaa cttcatgttc gtggatcgca
      781 atctcgtgca caagctcgat gtctcggaga tgtcgcaggt atacgaatgc atgcaggatg
      841 ccttgcgcga agcctgccca agcctgtcgg cccgagaagt tcgccggcgg tttcgcttcg
      901 ccatgggcac catcatgcat gaggtgcgct cccacgatga ccggcgcacg gcagaagccg
      961 gcgaggcggt ggtggaggat ctgctcgagt tcgtagccgg ggggttcgcg tatggtcagg
     1021 tttgagctgc gctacgcgct gcgccagccg gagttcaagg gcgacgactt tcagccccgt
     1081 tatcaggcct gcatcgaaca gtgcgcgtgg ggagatcggc tgggtttttc ggccgtgatg
     1141 atctcggagc atcatggttc cccggacggt tacatgccgt cacccatggt cctgggtggt
     1201 gccatcgcgg cggtcacgga gcacatgcgc atccgcatcc tgtcgcttgt cgggccgctg
     1261 cacaatccgg ttcgtctggc cgaggacctg gcaactctgg atctggtttc caacgggcgg
     1321 gtggatccct gcatcagcgg cggctacgtc ggctacgagt tcgaagccat gggtacctcg
     1381 ctgacttgcc ggaaggagta catggaagag atcgttccat tcctccacaa ggcatggacg
     1441 ggcgaaccct tcgagtggaa gggcaggacc atccgggtga cgccgcggcc ggtgcagagg
     1501 ccgggtcccc caatctggat gggaggcgcg tcgaaagcgt ccgcccgccg cgcggcccgt
     1561 catgccgatg tatttctgcc tgcccatccg gacctgatga agacctatcg cgaggagctg
     1621 gggaagctcg gcaggccacc caaggagccg cggggccggt ccatgctcgt ctggcttgcg
     1681 gaagatccgg acaggttctg ggcggagttc gggccaagcg cgctgcacga gaacaatgcg
     1741 tatggccagt ggtacgaaga ctggggtgcc tggaacggtt acgtgaaaga ggaaagcacc
     1801 gacgcgctgc gggcaacgga gcgttatccg gtcatgaccc cggatgagct ggtggcgcga
     1861 atcgaggagc gaggcggcaa catgaccgtg atgtttcacc cgatggccgg gggcgccgag
     1921 ccggaactgg catggcagag cctgcagctg atcgaagaca aagtgatccc ggcattgcgg
     1981 gacaaaggga tcgaaacggg aggatcgcaa tgagtgacta cgacatactg gttcgcggtg
     2041 gcaccgtcat agacggcacc atgatcccgc gctatcgcgc ggacgtcggc atcaaggacg
     2101 gcagggttgc caggatcggc gtcggccgca aagccactgc agaaagggtg atcgacgccg
     2161 gcgacaacat cgttgctccg ggatacatcg acatccacac ccactacgac gcccagatct
     2221 tgtgggatcc ctactgcacg atctctggct ggcacggggt cacctcggtg gttctcggaa
     2281 actgcgggtt cggcttcgcg ccggtcccgc cgcatctgcg cgagcgcgcc atgctgatga
     2341 tgactcgcaa cgaagccatc ggcttcgaga ccatgaaaga ggggatggag tgggacaggt
     2401 acggctggga aaccatgccg gagtggatcg agcatttgaa gcgcaatgtg cgcctcggcg
     2461 tcaacgccac cgccctggtg cccctcaacc cgctttacgc ctacgtgatg ggcggaatcg
     2521 acgaggccaa ggagcgcagg cccacggcgg cagagaagga ggagatgatc cgcctcatgt
     2581 acgaagccct cgatcacggt gcgtgcggct tttcgtacca gcgatgcggg gtgccctcgg
     2641 ttcagcccga ctgggacggt tcgccgatga tcacggacat gctgccggac gccgagatca
     2701 tcgagttcgg cagggcgctg ggcgattacg gccgcgggtt catcgagatg ttcgatgcgg
     2761 cgccctcgga ccacgatacc gtcgaggcct tcatgacctc cctggcccgc gcgagcggac
     2821 gtcccatcgt tcgcaacatc ctgcttgcca acgacgagta tccaaaggcg caccgggcct
     2881 ttctggaatg gctgaacgag gcccacgagg aagggcttca ggtgttcggc atgggtttca
     2941 ccgtgcgctc gcccaccgtg ctgacgttcg aggactggag cctctgggac ggcttccctg
     3001 gctggaacag ggtgatgaac ggccccaaag agcagcgggc cgagctcatg cgtaacgaga
     3061 gcatgcgggt gcagctccgc aaggagatgg acaccggccg cgtcggcggg ctcgggacca
     3121 gcggcaaacc ggcgaagctg atcatcaacg acacggccgg gtacggggag ctcgatcact
     3181 atctgggccg aatggtgtcc gagatcgcgg agaccgagaa caagcacatc gtcgacgtga
     3241 tcctcgacgc ggtgctggca tccgacttca aggtggagtt tcgcggcaac gcttacgaca
     3301 cctcggtgga gaccaccatg gaggtgctca acagccccta cgtcgtgccc ggcatctccg
     3361 acggcggcgc ccacacccac ttctccgtgc agggggcgta tcccaccgat ctgctggagt
     3421 ggctcgtgcg agaggagggc gtgatgaccg ctgagcgtgc ccactacggg ctgagccgcc
     3481 tgccggcgca catgtgcgat ttgggcgatc gcggcatcat tcgcgagggc gcgcctgcgg
     3541 atctggtgat ctacaacccg gaaaccattc gccgcacgcc ctcctgggac acgctcgaca
     3601 agctgcgcga cctgccggcc aacgagtggc gcctggtgca gcgcgcggag ggcctgaact
     3661 acaccctcgt gaacggtgtg gtcaccttcg agggcctgga gtgcacgggc gcgacccccg
     3721 gtgagctgat gctgcacggg gaggtctgag agttggcgac ggacgccggc cacgggggag
     3781 cggcgatgga ggcgcaggca gacgaaatcg gtcggctgcg tgaggaggtg gcccggctgg
     3841 gcgcgcggct gcagcacctg gaagcggttc aagcggtgcg ggagacgttg ctggactacg
     3901 ctcactacca cgatcgaggc ctttccggcg agctggcaga gctttttgcc gaagatgccg
     3961 tgctggacat ctccggattt ggcgatgatc tggacgtctc gatttccggg cgtggcgcca
     4021 ttcgtgacat gtacgcgagc ctggatgcgc gctcggatgg cccgccgccc tacaagcatg
     4081 cgataaccaa cctgcgcatc gagatcgacg gtgagcaggc tttggcgtcg agctacctac
     4141 tggattgggg tggccccgcc accgatctcg gaccgggagg cagcgtctac cgccagcgcc
     4201 tcgagcgaca gagcgacggg cgctggctca tcacacacaa acgcatcatc tgcaccgcgg
     4261 agctcaccgt ggaagcagcg tcgtcgttgc caaggtagcg cagcaatccg ccaccagaac
     4321 gggagacttc tatggcaggc aagctcgagg gcagggtcgc ggtggtgacc ggtgcgggac
     4381 gcggcgtcgg tcgcgctgtc gcgctgctgt tcgctcgcga aggcgcgaag gtggtcgtt
//
LOCUS       DBNHQR010000407         1074 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1684719, whole genome shotgun sequence.
ACCESSION   DBNHQR010000407 DBNHQR010000000
VERSION     DBNHQR010000407.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1684719"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="AC16VR_09245"
     CDS             complement(<1..198)
                     /locus_tag="AC16VR_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011381.1"
                     /translation="MAEQIHNFGQADPAADETWQLEAQEWLEAMDDVLKERGPEGART
                     LMRQLQTHLSHRGLVLTDAALN"
     gene            complement(393..>1074)
                     /locus_tag="AC16VR_09250"
     CDS             complement(393..>1074)
                     /locus_tag="AC16VR_09250"
                     /inference="COORDINATES: protein motif:HMM:NF015338.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR6011382.1"
                     /translation="KGWKHVLPHDSRTLTLAGIADVVAGFDFVALQEVDAGSIRSRFV
                     NQVEFLAERAGFPYWYTQLNRDLGPIAQHGNGLLSRIALQGMEDHRLPGAIPGRGAIV
                     VRLPFGDASVLVVMLHLSLGERSRGLQLEYVKRLIDGEQHVVVMGDMNSHLTDLLFDS
                     PLAETSLRPADSVGPTYPSWRPSQALDHILVSPSLAIDKCEVLDCRLSDHRPVSVHLS
                     RTDRTLVM"
BASE COUNT          182 a          357 c          358 g          177 t
ORIGIN      
        1 gttgagggcc gcgtcggtca gcaccagacc gcgatgggag agatgcgtct gcagctggcg
       61 catcagggtg cgggcgccct ccgggccacg ctccttgagt acgtcgtcca tggcctcgag
      121 ccattcctgg gcctcgagct gccaggtttc gtccgcggca ggatctgcct gaccgaagtt
      181 atggatctgt tccgccatct gtcttccccc agctgccgct tttcccatca agcccgcgcg
      241 ctggcgagcg ccggcttccg gatccctaat catagcccgc cgacgacgct ttcacgctcg
      301 caggcgggcg ccggacccgg atctggctgc cgaaccccca aacagaaacc gcgcccattg
      361 gcgcggttct cctctccggc tggagcccgg gatcacatca cgagcgtccg atccgtgcgg
      421 ctgagatgca cggaaacggg ccggtgatcc gacaaccggc agtcgagcac ctcgcacttg
      481 tcgatggcca gactcggcga taccaggatg tggtcgagcg cctgcgatgg gcgccaagac
      541 gggtaggtgg ggcccaccga gtccgccggc cgcaatgagg tttctgccaa cggggaatcg
      601 aacagcaggt ccgtaaggtg gctgttcatg tcgcccatca ccactacgtg ctgctcgccg
      661 tcgatgagac gcttgacgta ctcgagctgg aggccgcggg agcgctctcc gagcgagagg
      721 tggagcatca ccacgagcac gctggcgtcg ccgaacggca agcggaccac gatggcacca
      781 cggccaggga tcgcccctgg gagccggtgg tcctccatgc cctgcagcgc tatccgcgac
      841 agcaggccgt tgccgtgctg ggcgatggga ccgaggtccc gattgagctg ggtgtaccag
      901 taggggaagc cggcacgctc ggccaggaat tccacctggt tgacgaagcg gctgcgaatg
      961 ctgccagcgt ccacttcctg cagcgccacg aaatcgaagc cagccaccac gtcggcgatg
     1021 ccggcgagcg taagggtgcg gctgtcgtgg ggcaggacgt gcttccagcc cttg
//
LOCUS       DBNHQR010000408        10028 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_292124, whole genome shotgun sequence.
ACCESSION   DBNHQR010000408 DBNHQR010000000
VERSION     DBNHQR010000408.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10028)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10028)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10028
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_292124"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2045)
                     /gene="cofG"
                     /locus_tag="AC16VR_09255"
     CDS             complement(<1..2045)
                     /gene="cofG"
                     /locus_tag="AC16VR_09255"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF004884.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase CofG"
                     /protein_id="HIR6011383.1"
                     /translation="MALKTLIDDIRALALAGGLPSPAQALALADEPDTAALAAIAAEV
                     RDRGFQNVVTYSRKLFIPLTHLCRDVCHYCTFAQVPRKLKAPYMSVDEVLEIARHGAA
                     MGCKEALFTLGEKPELRYRAAREALAELGFETTLDYLAHVAEIVYRETGLLPHLNPGT
                     MTPEELARLRPLSPSMGIMLESTAERLGEKGMPHHGSPDKVPAVRLETLDHAGAARVP
                     FTTGILIGIGETRRERIESLLAIREVHERHGHVQEIIVQNFRAKPETKMARAPEPDLN
                     ELLWTIAVTRILFGSAMSVQAPPNLSPGVLAQIVAAGINDWGGVSPLTPDFVNPEAPW
                     PHLDDLARETAAAGKDLFERLTVYPGYVRDLDTWVAPELQERVLELSDSEGFARTSQW
                     CPGAEVEPPARLLESVTRPPERVSADLERILRKAGDGRRLEASEVVRLFQARGEDFSA
                     VVQAADGLRRETNGDVVSFCVNRNINYTNICYFKCQFCAFSKGKLSENLRGRPYDLSH
                     EEIARRTQEAWSRGATEVCMQGGIHPEYTGETYVGILQAVKEAVPEMHIHAFSPLEVW
                     QGAATLGLDLDTYLERLKAAGLGTLPGTAAEILDDEIRQIICPDKINTEQWLDVMRAA
                     HRVGFKTTATIMYGHVESAVNWARHLLRIRDLQAETGGFTEFVPLPFVHMEAPMYLKG
                     "
     gene            complement(2049..2720)
                     /gene="cofC"
                     /locus_tag="AC16VR_09260"
     CDS             complement(2049..2720)
                     /gene="cofC"
                     /locus_tag="AC16VR_09260"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:NF014085.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HIR6011384.1"
                     /translation="MWAVVPLKQLEQAKARLANVLTAEERRALMLAMARDVLTALGRS
                     KRLTGILIVSRTPEADALAQAFGTERFAEGPGANLSQALVQASEHLASRLEARGVMIV
                     PADVPLITPAEIDAILEQQDRVTVVPDDENVGTNCLICSPPNCIPYVFDGSSFKPHVD
                     AAFSLGITPTIMPSAGFALDIDTPDDLRTLLDRDPACQTGSYLAKSGIAERLSDADNG
                     SLTSR"
     gene            complement(2759..3688)
                     /gene="cofD"
                     /locus_tag="AC16VR_09265"
     CDS             complement(2759..3688)
                     /gene="cofD"
                     /locus_tag="AC16VR_09265"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HIR6011385.1"
                     /translation="MSGYLAITGGVGGAKLGLGLAQILPPDELTFAVNTGDDFEHLGL
                     HIAPDVDTLTYTLAGLSNTETGWGRADETWDFMDALDGLGGETWFRLGDRDLALHVMR
                     THLLSAGACLTEVTDHICRALGVAHRVLPMSDDPVRTTVHTERGPLPFQHYFVRERCE
                     PAVTGFEFAGIGQARLNPAITSRLDGLRGIIICPSNPFVSIDPMLNLRGARAALKESG
                     VPIVAVSPIVGGAAIKGPTAKMMRELNVPATAAQVAEHYGDLLCGLVLDEQDAHLEGT
                     LSTPTIVAQTVMVTLQDRVDLGRTVMDFIEQLS"
     gene            complement(3688..4482)
                     /gene="cofE"
                     /locus_tag="AC16VR_09270"
     CDS             complement(3688..4482)
                     /gene="cofE"
                     /locus_tag="AC16VR_09270"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HIR6011386.1"
                     /translation="MDKPEATSRTASPGFSVLGIPGVPMVSPGDDLTAIIVDALRDAR
                     ITLEDGDIVCVAQKIVSKAEGRQIPLASVTPSEEAQTLAEETDKDPRLVELILRESSE
                     VMRKKEGVLIVRHRLGLVGANAGIDQSNISHEDGEQALLLPEDPDRSAAELKSALYEA
                     SGARVGVIITDSANRPWRLGTVGIAIGCAGISVLDDHRGGIDLFDRELKVTLISRADG
                     IAGAATLVMGETTERIPVALVRGFPAEQSTDDARMVCRPLHEDLFR"
     gene            complement(4493..5152)
                     /gene="npdG"
                     /locus_tag="AC16VR_09275"
     CDS             complement(4493..5152)
                     /gene="npdG"
                     /locus_tag="AC16VR_09275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0006740 - NADPH regeneration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HIR6011387.1"
                     /translation="MAETIAILGGTGDLGTGLAIRWSKAGHKIIIGSRTLEKAQNAVG
                     NLHKISPETPADAMVNADAAAAGDIVVLTVPAEHQLSTLEGVKQSLTGKILIDVTVPL
                     VPPKVGTVQLPPEGSAGKRAQDMLGEDVMVVTAFQNIAAHLLQEDVHIECDVLVAGNK
                     KAARDKVIELVEQAGMKGYHAGPIENSAAAEALTSILIQINRRHDISHSGIKIVGQSD
                     H"
     gene            complement(5233..5700)
                     /locus_tag="AC16VR_09280"
     CDS             complement(5233..5700)
                     /locus_tag="AC16VR_09280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6011388.1"
                     /translation="MASFTDLSEKFTEITERIVWCTVSTVDAKGRPRSRILHPVWEGA
                     TGWIATGRHSHKARHLANNPYVSLSYWDPQHEQTMIECKAEWQDDQPTRDRIWELLKT
                     TPEPVGYDPALFWSSAADENFGVLKLTPWRLEVWSLAAMSAGKPAQVWRQEVG"
     gene            5811..6722
                     /locus_tag="AC16VR_09285"
     CDS             5811..6722
                     /locus_tag="AC16VR_09285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6011389.1"
                     /translation="MKIGLMMGATGTTTLDDVVALAKSAEAAGLDSLWMANIFSFDAI
                     STLAIIGRETSTIGLGTAVTPTYPRHPTAIAQQALTTAAASGSRFTLGIGLSHKIVIE
                     NMLGFSYDRPARHMREYLSVLMPLVRGETCNFQGEQYRVAGVRIDIPGEITMPVVVAA
                     LGPAMLKITGELADGTNTWMVGPKTMAEHIIPRISAAADEAGRPPPRIVGGYPVILTN
                     KPDEAREKIARELTIYGQLPSYRAMLDREGVAGPADIAIAGDENLVRGELKRLEDMGV
                     TDFNAAIMPVEEGAQERTVSFLASLKG"
     gene            complement(6752..7957)
                     /locus_tag="AC16VR_09290"
     CDS             complement(6752..7957)
                     /locus_tag="AC16VR_09290"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HIR6011390.1"
                     /translation="MIARTGVLLVSMLWAASAKAEPVGFAACVSNLESVAVASGISEA
                     TAKQVLGEVRHLERVIELDRTQPEFVRTFSEYYRVRVTPSRVADGRRLLEENAALLGR
                     LQRQYGIPGHYLIALWGLETNFGSYFGNMSTMDSLATLACDERRSAFFTAELMDALRI
                     VDAGDMEPAAMQGSWAGAMGHVQFMPSVFRRYAVDDDGDGRRDLWTSRRDALASAANY
                     LKQAGWERGFRWGREVRLPEAFDYAGAARDPTRPLADWEAAGVRNAFGGPLASTDLPA
                     TLRLPSGHRGPAFLTYPNFEVIMQWNRSESYALSVGNLADRIAGRSPLTRPPPTDELK
                     LTPDSVRQLQADLNALGLDAGEADGVIGPATRAAIGRFQIGHGLVPDGYPDHRLVEAL
                     RASREAGER"
     gene            complement(7965..9335)
                     /locus_tag="AC16VR_09295"
     CDS             complement(7965..9335)
                     /locus_tag="AC16VR_09295"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6011391.1"
                     /translation="MTQPRLSKATLFCYGVADLPVMLAIMPMSIWLSRFYTGDMGLSL
                     SAVANIMLLARLFDVFTDPLVGHLSDHTRTRWGRRKPWMLASLPLLMIGLYKVFLPPD
                     GIGIWYLFSWMMVMWLGWTMLMIPYYAWAAELSDDYDERTRITGWRAVMGSVGGIGAQ
                     LIPFIALVLFGFGGTANVMTMLGIAALILMPLCVGVALLRVPEFPEMRAPSVPILAGI
                     RIMWRNGPFRRLLLAFVLSSTGLAIVMPLYIFFVEFIVEEPPANVPYMIIISSVAGFL
                     GIPFWVWLSKHVGKHKAWIGGFLVVAAVSPMYLFLGPGDFWLMVPGIIIIGIGTGSFS
                     ALPNSMKADVIDLDTARSGKNRAAFFFSAWSLVTKLASSLGGWLALQSLALFGFDAAN
                     GAQNTPEALTGLRLTFAVLPAIIFVIAAAVIWRYPITKERQARIRAAIDRRTLRRNAA
                     QAGFAK"
     gene            complement(9528..>10028)
                     /locus_tag="AC16VR_09300"
     CDS             complement(9528..>10028)
                     /locus_tag="AC16VR_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011392.1"
                     /translation="VAGLDNYYSSGLGAGLDAQFQATGDCISAIVMSEQLRNGVIGNT
                     GDDQLDAVVEGFSCTLNKGADLDDVRSAAEFLNKQIDAIGSAALSQYEAYLWTPYRGG
                     TNTADFLWVGNYPNLSTWAQGDTDYYASKEGQAAEERFNKISTCTNAIWGGYWIVPPA
                     EGPTAQ"
BASE COUNT         1655 a         3253 c         3384 g         1736 t
ORIGIN      
        1 cccttcaagt acatgggcgc ttccatgtgg acgaacggga gcggcacgaa ctccgtgaaa
       61 ccgccggttt ccgcctgcag atcgcggatc cgcagcaggt gccgggccca gttgacggca
      121 ctctccacgt gcccgtacat gatggtcgcc gtggtcttga accctacccg atgggccgcg
      181 cgcatgacgt ccagccactg ctcggtgttg atcttgtccg ggcagatgat ctgccggatc
      241 tcatcgtcga ggatctccgc cgccgttccg ggcagggtac ccaggccggc cgccttgagg
      301 cgttcgaggt acgtatccag atccagcccc agcgttgctg cgccctgcca gacctccagc
      361 ggggaaaacg cgtggatgtg catctcaggg acggcttcct tgaccgcttg gaggatgccc
      421 acataagtct cacccgtgta ttcggggtgt atgccgccct gcatgcacac ttccgtagcg
      481 ccccgggacc atgcttcctg ggtacggcgg gcaatctcct cgtgggacag gtcgtaggga
      541 cgcccgcgaa gattctccga cagcttgccc ttggagaacg cgcagaactg gcacttgaag
      601 tagcagatgt tggtgtagtt gatgttgcgg ttgacgcaga agctcaccac gtcgccgttc
      661 gtctcccggc gcagcccgtc ggctgcctga accacggcgc tgaagtcctc gccccgcgct
      721 tgaaacagac gcaccacctc ggacgcctcc agccgccggc catcgcccgc tttgcggaga
      781 atacgttcga gatccgccga gacgcgctcg ggtggccgcg ttacggactc gagcagcctc
      841 gccggtggct ccacctcggc acccggacac cactggctgg tgcgggcaaa gccttcgctg
      901 tcggagagct ccagcacgcg ctcctgcagc tcgggtgcaa cccatgtgtc gagatcccgg
      961 acgtagcccg gatagaccgt caggcgttcg aaaaggtctt tgccggccgc cgccgtctct
     1021 cttgcgagat cgtccaggtg cggccacggc gcttccgggt tgacgaaatc cggcgtcagc
     1081 ggcgagacgc cgccccagtc gttgatgccg gccgccacga tctgtgcgag cacccccggg
     1141 ctcaagttcg ggggcgcctg cacgctcatg gcggacccga acaggatgcg ggtcaccgcg
     1201 atggtccaga gcagctcgtt gaggtcgggc tcgggtgcgc gggccatctt ggtctcgggc
     1261 ttggcccgga agttctggac gatgatctcc tgcacgtggc cgtggcgctc gtgcacctcg
     1321 cggatggcca gaagcgattc gatgcgctcc cggcgggtct cgccgatacc gatgagaatg
     1381 ccggtggtga acggaacccg cgcggcgccg gcgtggtcga gggtctcgag acgcacggcg
     1441 ggcaccttgt ccggcgagcc gtggtgaggc atccctttct cgcccagccg ctcggctgtg
     1501 gattccagca tgatgcccat ggatggcgag agggggcgca gccgggcgag ctcctcgggg
     1561 gtcatggtgc cggggttgag gtgcggcagc aggccggtct cccgatagac gatctccgcc
     1621 acgtgggcca gatagtcgag cgtggtctcg aaacccagtt cggcaagcgc ttcccgggct
     1681 gctcggtaac gcagctcggg tttctcgcct agggtgaaca gggcttcctt gcaacccatg
     1741 gcggcgccgt gacgggcgat ttcgagcacc tcgtccacgc tcatgtaggg cgctttgagc
     1801 ttccgcggga cctgggcgaa cgtgcagtag tggcagacgt cccgacacag atgggtgagc
     1861 ggaatgaata gctttctgga ataggtcacc acgttctgga agccgcggtc gcgtacttca
     1921 gcggcgatcg ccgccagggc ggcggtgtcc ggctcgtcag ccagcgcgag ggcctgtgcc
     1981 gggctgggaa gcccgccggc cagggccagg gcgcgaatgt cgtcgatgag cgtcttcagg
     2041 gccatttcct acctcgaggt cagcgagcca ttatcggcat cgctaagccg ctcggctata
     2101 cctgatttgg caagatacga gcccgtttgg caggccggat cgcggtccag caaagtgcgc
     2161 aggtcgtcgg gggtatcgat gtccagggcg aagccggcgg agggcatgat ggtgggcgtg
     2221 atgcccaggg agaacgcagc gtccacatgg ggcttgaaac tggaaccgtc gaagacgtag
     2281 gggatgcagt tgggtggtga gcagatcagg cagttcgtgc cgacgttctc gtcgtccggg
     2341 accacggtga cccgatcttg ctgctcgagg atcgcgtcga tctccgccgg cgtgatcagc
     2401 ggcacgtcgg cgggcacgat catcacgccg cgggcttcca accgcgaggc gaggtgctcg
     2461 ctcgcctgga cgagggcctg ggaaagattc gcgccggggc cttccgcgaa gcgctccgtg
     2521 ccgaaggcct gggccagggc gtcggcttct ggtgtacggg atacgatgag gatgcccgtc
     2581 agccgcttgg agcggccgag ggccgtgagc acgtcgcgcg ccatggcgag catgagcgcc
     2641 cggcgctcct ctgccgtcag aacgttggcc agccgcgcct tcgcctgttc gagctgtttc
     2701 aacggtacca cggcccacat ggggaggttc tctccgtctt gcttggtttc agcttgaatc
     2761 agctgagttg ttctatgaaa tccatgaccg tgcggccgag atccacacgg tcctgcaacg
     2821 ttaccatgac ggtctgggcc acgatagtcg gggtactcag cgtgccttcc agatgcgcat
     2881 cctgctcgtc gagcacgagt ccacagagca gatcgccgta gtgctcggcc acctgagccg
     2941 cggtcgccgg gacgttgagc tcgcgcatca tcttcgcggt gggccccttg atggcggctc
     3001 cgccaacgat gggagagacc gccacgatgg gcacgccgct ttcttttaga gcggcgcggg
     3061 cgccgcgcag gttcagcatg gggtcgatgg agacgaacgg gttggagggg cagatgatga
     3121 tgccgcgaag gccgtccagg cgcgacgtga tcgccgggtt gagccgcgcc tgcccgatcc
     3181 cggcgaactc gaacccggtc accgccggct cgcaccgttc ccgcacgaag taatgctgga
     3241 aaggcagggg cccccgttca gtgtgcacgg tcgtgcgcac aggatcgtcg gacatcggca
     3301 gcacccggtg ggcgacgccc agggctcggc agatgtggtc ggtgacttcg gtgaggcaag
     3361 caccggccga cagcaggtgg gttcgcatca cgtgcagcgc caggtcccga tcgccgagac
     3421 ggaaccaggt ctcaccgccg aggccgtcca gggcatccat gaaatcccag gtctcgtcgg
     3481 cgcgccccca gccggtctcg gtgttgctca gccccgccag ggtataggtg agggtgtcga
     3541 cgtccggcgc gatgtgcaga cccaggtgct cgaagtcatc gcccgtattc acggcgaagg
     3601 tgagctcgtc cggcggcagg atctgcgcga gaccgagccc cagtttcgcc ccgcccacgc
     3661 cacccgtgat ggcgagatac ccggacatta tcgaaacaga tcctcgtgca gaggtcggca
     3721 gaccatgcgg gcgtcgtcgg tggattgctc ggccgggaag ccgcgcacca gggccacggg
     3781 aattctctcc gtggtttcgc ccatgaccag ggtggcggcg ccggcgatgc cgtccgcccg
     3841 gctgatgagg gtgaccttca gctcccggtc gaacagatcg atgccgccgc gatgatcgtc
     3901 gagcacgctg atgcccgcgc agccgatggc tatgcccacg gtgcccagcc gccaggggcg
     3961 attggcgctg tcggtgatga tcacgccgac ccgggcgccg ctggcctcgt agagggcaga
     4021 cttcagctcg gccgccgagc ggtccgggtc ctcgggaagg aggagcgcct gctcgccatc
     4081 ctcgtgggag atgttcgact ggtcgatccc cgcgtttgct ccgaccaggc caaggcgatg
     4141 gcgcacgatc agcacgccct cttttttccg catcacctct gacgattcgc gcaggatcag
     4201 ctcgacgagc cgcgggtcct tgtccgtttc ctccgccagc gtctgggcct cctcgctcgg
     4261 cgtcaccgac gccagaggta tctggcgtcc ctcggccttg gagacgatct tctgcgcaac
     4321 gcagacgatg tcgccgtctt ccagggtgat gcgggcatcg cgcagggcgt cgacgatgat
     4381 ggccgtcagg tcgtccccgg ggctcaccat gggcacgccc gggatgccca gcacggaaaa
     4441 gccgggcgat gccgtcctgc tggtggcttc cggcttgtcc attcgcgttt ggtcagtgat
     4501 ccgattgacc gacgatcttg atcccggagt gggagatgtc gtggcgcctg ttgatctgaa
     4561 tcaggatcga ggtgagcgct tcggccgcgg cggaattctc gatgggcccc gcatggtagc
     4621 ccttcatgcc ggcctgctcg accagctcga tgaccttgtc ccgggcggcc ttcttgttcc
     4681 ccgccaccag cacgtcgcac tcgatgtgca cgtcttcctg aaggaggtgg gcggcgatgt
     4741 tctgaaaggc ggtaacgacc atcacgtcct cacccagcat gtcctgggcc cgcttgccgg
     4801 cgctgccctc gggcggaagc tgcacggtgc cgaccttcgg gggaaccagg ggcacggtca
     4861 cgtcgatgag tatcttcccg gtgagcgatt gcttgacgcc ctccagcgtc gacagctgat
     4921 gctcggcggg aacggtcagg acgacgatgt ccccagccgc ggcggcatcg gcgttcacca
     4981 tggcgtcggc tggcgtttcc gggctgatct tgtggaggtt gcccaccgcg ttctgcgcct
     5041 tctccagcgt ccgcgagccg atgatgatct tgtggcctgc tttggaccac cgtatggcca
     5101 gcccggtgcc gagatctccg gtaccgccaa gtatggctat ggtctctgcc atgtctttct
     5161 cccgcttgtt gctcattgcc ggcgcgcccg ggtgcaggcg ctgacggcgg tgaaaagcgg
     5221 gcgattatgc cgctaaccga cttcctggcg ccatacctgc gccggcttgc cggcggacat
     5281 ggcggccagg gaccagacct cgagccgcca gggtgtgagc ttgaggacac cgaaattctc
     5341 gtccgcagcg cttgaccaga acagcgcggg gtcgtagccc acgggctccg gcgtggtctt
     5401 cagcagttcc cagatccggt cccgggtggg ctggtcgtcc tgccactcgg ctttgcattc
     5461 gatcatcgtc tgctcgtgct gaggatccca gtagctcagc gagacgtagg ggttgttcgc
     5521 gaggtgccgg gccttgtgcg agtggcggcc cgtggcaatc cagcccgtgg cgccctccca
     5581 gaccggatgg aggatgcgcg agcggggccg gcccttggcg tccaccgtgc tgaccgtgca
     5641 ccacacgatg cgctccgtga tctctgtgaa cttctcgctg agatccgtaa atgacgccac
     5701 gtccactcgc cggcctcccc gttcgcctcc aggcggcttt gttgccggcc ctggtttgcc
     5761 ttattgtgcg gcatttcccg acctgtacaa ccatcgccta gggggcagaa atgaaaatag
     5821 gactcatgat gggcgccacc ggcaccacca cgctcgacga cgtcgtcgct ttggcgaaga
     5881 gcgctgaagc ggccgggctc gacagcctct ggatggcgaa catcttctcc ttcgatgcca
     5941 tatccaccct ggccattatc ggccgcgaga ccagcaccat aggtcttggc accgcggtta
     6001 cgcccaccta cccgaggcac cccacggcca tcgcccagca ggccctgacc acggcggccg
     6061 ccagcggcag ccggtttacc ctgggtatcg ggctttctca caagatcgtg atcgagaaca
     6121 tgctcggctt ctcctacgac cgacccgcgc gacacatgcg tgaatatctc agcgtgctga
     6181 tgccgctcgt tcggggggag acctgcaatt ttcagggcga gcagtaccgg gttgccggcg
     6241 tgcggatcga catccccggc gagatcacga tgcccgtggt ggtcgccgct ctcgggccgg
     6301 cgatgctcaa gattaccggt gagctggccg atggcaccaa cacctggatg gtgggcccga
     6361 agaccatggc cgagcacatc atcccccgga tcagcgccgc tgcggacgag gccgggcgtc
     6421 cgccaccgcg catcgtgggc ggttacccgg tgatcctgac caacaagccc gacgaagcac
     6481 gggagaagat cgcccgggaa ctcaccatct acggccagct tcccagctac cgcgccatgc
     6541 tcgaccggga gggggtggcg ggccccgcgg acatcgccat tgccggagac gaaaacctgg
     6601 tgcgcggcga gctcaagcgg ctcgaggaca tgggggtgac ggatttcaac gccgcgatca
     6661 tgccggtgga ggaaggggcg caggagcgca cggtgtcgtt cctggccagc ctcaagggct
     6721 gagtcccggc ttcagcgatc ccttttgagg ttcagcgctc gccggcttcc cggctggcgc
     6781 gcagggcctc cacgagccga tggtcgggat acccgtccgg gaccagaccg tgaccgatct
     6841 ggaaccgccc gatggcggcc cgggtcgccg ggccgatcac gccgtcggct tcgccggcat
     6901 ctaaccccag ggcgttcagg tccgcctgca gctggcgcac cgagtccggc gtcagcttca
     6961 gctcgtccgt gggtggcggc ctggtgagcg gtgaccgtcc ggcgatccgg tcggccaggt
     7021 tgccgacgct cagcgcgtag ctctccgacc gattccactg catgatcacc tcgaagttgg
     7081 gatacgtcag aaacgcaggc ccccggtgcc cggagggcag gcgcagggtg gccggcagat
     7141 ccgtggacgc cagcgggcct ccgaacgcgt tgcgcacccc ggcggcctcc caatccgcga
     7201 ggggccgcgt ggggtcacgg gcggcgcccg cgtagtcgaa cgcctcgggc aggcgtacct
     7261 cgcgccccca gcggaaaccc cgctcccagc cagcctgttt gaggtaattg gccgcagatg
     7321 ccagggcgtc gcgccggctg gtccagagat cccttctgcc gtcgccgtcg tcgtccacgg
     7381 cgtagcggcg gaagaccgaa ggcatgaact gaacgtggcc catggcgccc gcccaggagc
     7441 cctgcatggc tgcgggctcc atgtccccgg cgtcgacgat gcgcagggcg tccatcagct
     7501 ccgccgtgaa gaaggcgctg cggcgctcgt cgcaggccag cgtggcgagc gagtccatgg
     7561 tgctcatgtt gccgaagtag ctgccgaagt tggtctccag accccacagc gcgatcaggt
     7621 agtggccggg aatcccgtac tggcgctgca gccggccgag aagggcggca ttctcctcga
     7681 gcaggcggcg cccgtcggcc accctcgatg gggtcacccg cacccggtag tactcgctga
     7741 acgtgcgaac gaactccggc tgcgtgcggt cgagctcgat cacccgctcc aggtggcgta
     7801 cctcgccgag tacctgcttc gcggtcgcct cggaaatccc ggacgccacg gcaacggatt
     7861 ccaggttgct gacgcaggcc gcgaacccca cgggctcggc tttagccgac gcggcccaga
     7921 gcatggatac gagcagcacg cccgttcgcg cgatcaccgc ggcgttactt cgcgaagcct
     7981 gcttgggctg cgtttcggcg cagcgtgcgc cggtcgatgg ctgcccggat gcgggcctgg
     8041 cgctccttgg tgatcgggta gcgccagatc acggcggcag ctatcacgaa gatgatggcc
     8101 ggcagcacgg cgaaggtgag ccgcagcccg gtcagcgcct ccggcgtgtt ctgcgcaccg
     8161 ttggccgcgt cgaagccgaa cagggccaga gactgcagag ccagccagcc gccaaggctc
     8221 gaggccagct tggtcaccag ggaccacgcg gagaagaaga aggcggcccg gttcttgccg
     8281 ctgcgtgccg tgtccaggtc gatcacgtcg gccttcatgg agttgggcag ggcgctgaag
     8341 gacccggtgc cgataccgat gatgatgatg ccgggcacca tcagccagaa gtcgccgggg
     8401 ccgaggaaga gatacatggg gctcaccgcg gctaccacca gaaagccgcc aatccacgct
     8461 ttgtgcttcc ctacgtgctt ggagagccag acccagaacg gtatgcccag aaaaccggct
     8521 accgagctga tgatgatcat gtaggggacg ttggcaggcg gctcctcgac gatgaactcg
     8581 acgaagaaga tgtacagcgg catgacgatg gcgagcccgg tggaggacag gacgaaagcg
     8641 agtaacaggc gacggaacgg accgttgcgc cacatgatcc ggatgcccgc caagatgggc
     8701 accgacggcg cccgcatctc cggaaactcg ggcacccgaa gcagcgccac gcccacgcac
     8761 aggggcatga gaatgagcgc cgcgatacct agcatggtca tgacgttggc ggtgccgccg
     8821 aagccgaaca gaaccagcgc gatgaagggg atcagctggg cgccgatgcc tcccacgctg
     8881 cccatgaccg cgcgccagcc ggtgatgcgc gtgcgctcgt cgtagtcgtc ggagagctcg
     8941 gcggcccagg cgtagtaggg aatcatcagc atggtccagc cgagccacat gaccatcatc
     9001 cacgagaaca ggtaccagat gccgatgccg tcgggcggca gaaacacctt gtacaggccg
     9061 atcatgagca atggcagcga cgccagcatc cagggcttgc gcctgcccca tcgggtgcgg
     9121 gtgtgatcgc tcaagtgccc caccaggggg tcggtgaaca cgtcgaagag cctggccagc
     9181 agcatgatgt tggccaccgc cgacaggctc aggcccatgt cgccggtgta gaagcgcgac
     9241 agccagatgg acatgggcat gatcgccagc atcaccggaa gatcggcgac gccgtagcag
     9301 aacagggtgg ccttgctgag ccgcggctgg gtcaactgcg ttcccgattg gcgggcttgg
     9361 aggcttcagt gtaagtcggc cgcgcctgag gaatccacca tgactcggcg cgctctgggt
     9421 gctcggcgat cggccgctgg tcgtcaatcc cgtcgtgacg gttcccaagc cggcgacggc
     9481 gtcatgccga ccctggtgtg cttccagggc cggcatggtg gagtaaccta ttgggcggtg
     9541 ggtccctcgg cgggcggtac gatccagtag ccgccccaga tggcgttggt gcaggtgctg
     9601 atcttgttga accgctcctc ggccgcctgg ccctccttgg aggcatagta atcggtgtcc
     9661 ccctgggccc aggtcgagag gttcgggtag ttacctaccc ataggaaatc ggcggtgttg
     9721 gtccctcccc gatagggagt ccacaggtac gcctcgtact ggctcagcgc ggcggagccg
     9781 atggcgtcga tctgcttgtt cagaaactcc gcggcgctgc gcacgtcgtc caggtcggca
     9841 cccttgttga gggtgcagct gaaaccttcg accacagcgt ccagttgatc gtctccagtg
     9901 ttcccaatca cgccgttgcg gagctgctcg gacatgacta tcgcggagat gcagtcgccg
     9961 gtagcctgaa attgcgcgtc gagtccggcg cccaggcccg acgagtaata attatcgaga
    10021 ccggcgac
//
LOCUS       DBNHQR010000409         2918 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1621826, whole genome shotgun sequence.
ACCESSION   DBNHQR010000409 DBNHQR010000000
VERSION     DBNHQR010000409.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1621826"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="AC16VR_09305"
     CDS             <1..490
                     /locus_tag="AC16VR_09305"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014578086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR6011393.1"
                     /translation="KALTVARDRVVRFHYVIRDEAGSELESTREGEPLAVLQGRGSVL
                     RGVEEALEGRNAGERLEVTLPPEQAFGARREDWTQRVPKKYLPDAKRLKPGDAAVLRT
                     REGPRPVTVVKVGSKVVDVDLNHPRAGMTLTFDLEIVEVREATPEEIAHRHVHGPGGH
                     RH"
     gene            complement(500..2095)
                     /locus_tag="AC16VR_09310"
     CDS             complement(500..2095)
                     /locus_tag="AC16VR_09310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019750117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HIR6011394.1"
                     /translation="MIIGAGTAGCVLANRLSADKDADVLLLEAGGKDDYFWIDIPVGY
                     LYTINNPRTDWCYQVEPEPGLNGRRINYARGKVLGGCSSINAMIYMRGQRGDYDHWAQ
                     LGNRGWSWDDVLPVFKRSEDYQHGAGEFHGAGGELRVEERRVDWEILDAWRDAAQECG
                     IPKIEEFNRGDNFGNAYFQVNQRRGVRWSASKAFLRPAMARPNLEVLTNAHVQRLEIE
                     TTDAGLRASGVTVQMGDDAPTTITARREVVLAAGAIGSPQILQLSGVGPGDLLHEHGI
                     RVIHDLPGVGENLHDHLQVRSVYKVHGTVTLNQRANSLIGKALMGLEYFTRKTGPLTM
                     PPSQLGAFAKSDPSVASADIEWHVQPLSLDAFGEPLHRFNAITPSVCNLRPTSRGHLR
                     IKSPDPADYPAITLNYLATEHDRQVILAGMRLTRRIMAAKALERFQPEEWKPGPSLTT
                     DEELLTAAGDLGTTIFHPVGSCRMGSDPMAVVGDRLAVHGVEGLRVIDASVMPQITSG
                     NTNAPTVMIAEKGAEFMRADAAS"
     gene            complement(2118..>2918)
                     /locus_tag="AC16VR_09315"
     CDS             complement(2118..>2918)
                     /locus_tag="AC16VR_09315"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RHO alpha subunit C-terminal catalytic
                     domain-containing protein"
                     /protein_id="HIR6011395.1"
                     /translation="EVLPEHFKNWKLEDRFVALHTRKILPGNWKMCMEAFLEAYHVLA
                     THPEGIHSAGDANADYDIFGRHVTRFVHTIGYPSPHVASEVDERKLFAALGHNPDDLP
                     DGMSARQRHADIMREQLGREWDVDLSGLSNSEMLDSIEYFVFPNACFFPGINIRLVYR
                     FRPIDVDHTMHEILLLQPVPEHGERPKPAEPIDLTIDESYEKANEVSLGGFALAMVLD
                     QDTENFHRQRAGIKAAQRAGVRTAAVLANYQESRIRRLHMTVDEYMAG"
BASE COUNT          462 a          926 c         1025 g          505 t
ORIGIN      
        1 caaggccctc accgtggcgc gggatagggt ggtgcgcttt cactacgtga ttcgcgacga
       61 ggcgggctcg gagctcgagt ccacgcgcga aggcgagcct ctggccgtgc tgcaggggcg
      121 cggcagcgtg ctgcggggcg tcgaggaagc gctggagggc cgaaacgccg gcgagcgcct
      181 ggaagtaacc cttcctcccg agcaggcttt cggcgcccgg cgggaagact ggacccagcg
      241 cgttcccaag aaatatctgc ccgatgccaa acggctcaaa cctggcgatg cggccgtgct
      301 gcggacccgg gaggggccgc gtccggtcac cgtggtgaag gtggggagca aagtcgtgga
      361 cgtggatctc aaccatcccc gcgcggggat gaccctgacg ttcgatctcg agatcgtgga
      421 ggtgcgcgaa gccacgcccg aggagatcgc ccaccgccac gtgcacggcc ccgggggcca
      481 tcggcactga ggcggtgtct caggaggccg cgtcggcccg catgaactcc gcgcccttct
      541 cagcgatcat caccgtgggc gcgttggtgt tgccggaggt gatctgcggc attaccgaag
      601 cgtcgatcac ccgcagccct tcaacgccgt gcacggccag ccggtcgccg accacagcca
      661 tggggtcgct gcccattctg cagctgccca ccgggtgaaa gatggtggtg ccgagatcgc
      721 cggcggcggt gagcagctcc tcgtcggtag tcaggctggg gccgggcttc cactcctccg
      781 gttgaaaccg ctccagtgcc tttgccgcca tgatccggcg ggtgagccgc atcccggcga
      841 ggatgacctg acggtcgtgc tcggttgcga ggtagttgag ggtgatcgcc ggataatcgg
      901 cggggtccgg gctcttgatt cgcagatgcc cgcgactcgt gggccgcaag ttgcacaccg
      961 acggggtgat cgcgttgaag cggtgcagcg gctccccgaa ggcgtccagc gacagcggct
     1021 gaacgtgcca ctcgatgtcc gcggaggcga ccgacggatc ggacttcgcg aacgcgccca
     1081 gctgagaggg cggcatggtg agtggtccgg ttttcctggt gaagtactcc agccccatca
     1141 gcgccttgcc gatgagggag ttcgcccgct gattcaacgt caccgtgccg tgcaccttgt
     1201 agacggagcg cacctgcagg tggtcgtgga ggttctcccc cacgcccggc aggtcgtgga
     1261 tcacccggat gccgtgctcg tgcagcagat cgccggggcc aacaccggag agctgcagaa
     1321 tctgggggga gccgatggcg cctgcggcaa gcaccacttc ccggcgcgcg gtgatggtgg
     1381 tgggcgcgtc gtcgcccatc tgcacggtga cgccggaggc gcgaaggccg gcgtcggtgg
     1441 tctcgatttc caggcgctga acgtgggcgt tcgtgagcac ctcgagattg ggacgcgcca
     1501 tggccgggcg caggaaggct ttgcttgcgc tccagcgcac cccgcgccgc tggttcacct
     1561 gaaagtaggc attgccgaag ttgtcgccgc ggttgaactc ctcgatcttg gggatgccgc
     1621 actcctgggc agcatcccgc caggcatcca gaatctccca gtcgacgcgc cgctcctcga
     1681 cgcgcagctc gcctcccgcg ccgtgaaact cgcccgcgcc gtgctggtag tcctcggagc
     1741 gcttgaacac cggcagcacg tcgtcccacg accagccccg attgccgagc tgagcccagt
     1801 gatcgtagtc gccgcgctga ccgcgcatgt agatcatggc gttgatggac gagcagccgc
     1861 cgaggacctt gccgcgggcg tagttgatgc ggcggccgtt gaggccgggc tcgggctcca
     1921 cctgatagca ccagtccgtg cgcgggttgt tgatggtgta caggtagccg accgggatgt
     1981 cgatccagaa gtagtcgtcc ttgccgccgg cttccagcag caggacgtcg gcatctttgt
     2041 cagcgctcag acggttcgcc agcacgcagc cggcggtgcc cgctccgatg atgatgtagt
     2101 cgaactcgcg aacctgacta acccgccatg tactcgtcca ccgtcatgtg cagccggcgg
     2161 atccgggact cctggtaatt ggccaggacg gcggccgttc gcacacccgc cctctgtgcg
     2221 gccttgatgc ccgcgcgctg acggtgaaag ttctccgtgt cctggtccaa gaccatggcc
     2281 agcgcaaacc cgccgaggga gacctcgttg gccttctcgt atgactcgtc gatggtcagg
     2341 tcgatgggct cggccggctt gggccgctcg ccgtgctccg gcacgggctg cagcagcagg
     2401 atctcgtgca tggtgtggtc cacgtcgatg ggccggaagc ggtacaccag gcggatgttg
     2461 atgccgggga agaagcaggc gttggggaag acgaagtact cgatggaatc cagcatctcg
     2521 ctgttggaga gcccgctcag atccacgtcc cactcgcgcc caagctgctc acgcatgatg
     2581 tccgcgtggc gctgccgggc gctcatgcca tcgggcaggt cgtccgggtt gtggccgagg
     2641 gccgcgaaca gcttgcgctc gtccacctcc gaggcgacgt ggggggacgg gtagccgatg
     2701 gtgtgcacga agcgggtgac gtgacggccg aagatgtcgt agtcggcgtt ggcgtcgccg
     2761 gcgctgtgaa tgccttcggg atgagtggcg agcacgtggt aggcctccag gaacgcctcc
     2821 atgcacatct tccagttgcc cggcaggatc ttgcgggtgt gcagggccac gaagcggtcc
     2881 tcgagcttcc agttcttgaa gtgctcggga agaacttc
//
LOCUS       DBNHQR010000410         5958 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_545328, whole genome shotgun sequence.
ACCESSION   DBNHQR010000410 DBNHQR010000000
VERSION     DBNHQR010000410.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5958)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5958)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5958
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_545328"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="AC16VR_09320"
     CDS             complement(<1..853)
                     /locus_tag="AC16VR_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HIR6011396.1"
                     /translation="MVKAVAEFEVVSDYEPAGDQPTAIAGLVEGLVDGLSHQTLLGVT
                     GSGKTYTVANVIEAVQRPTLVLAPNKTLAAQLYGEFRDFFPNNAVEYFVSYYDYYQPE
                     AYVPASDTYIEKDSSINAHIEQMRLSATKALLERRDTIIVASVSAIYGLGDPQSYLRM
                     VLHLDRGDRVDQRYVLQRLAELQYTRNDMAFQRGSYRVRGDVVDIFPAESEKEAVRVE
                     LFDDEIENISVFDPLTGEIVNRVPRYTVFPKTHYVTPRERILAVLEDIKEELQDRLAY
                     LQKHHKLV"
     gene            complement(860..1234)
                     /locus_tag="AC16VR_09325"
     CDS             complement(860..1234)
                     /locus_tag="AC16VR_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011397.1"
                     /translation="MKVVVKTDEYTIYQRRDERYAVKDATKAWVNGDAKAAILVQHEL
                     ITAALPAAPLEEPEADAGEPAAEAAAAEEVTGAEESAAEESEPAEAAAPAEEAAAAEV
                     AAEDEAGEPASAEEGEEEPKPE"
     gene            1409..3262
                     /locus_tag="AC16VR_09330"
     CDS             1409..3262
                     /locus_tag="AC16VR_09330"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HIR6011398.1"
                     /translation="MVEEKTSYVIRFAGDSGDGIQLQGQQFTTASALAGHDLATMPDF
                     PAEIRAPAGTRYGVSAFQIQFGGDRVTTPGDAPDVLIAFNPAALVVNLSHLRPGALVV
                     VDRQAFTDQRLKRADLTSNPLDDGTLDGFELLDIDISDLTHQAVDALGLGAKDSLRCK
                     NFWALGLVLWMFDQKRDATVSWIEAKFAGNEQVRSANLAALNAGHAFGETAELGPKLR
                     QHAAPEASLASAEYRTLTGAEALALGLVAAGELADTELMFCSYPITPASSLLHQLTGL
                     EDAGVATFQAEDEIAAICSAIGASYAGKLGVTSSSGPGIALKTEAMGLAVAAELPLII
                     VNSQRAGPSTGMPTKTEQSDLYQAVYGRNGDTPLPVVASRSPADCFDTAIEAVRIALR
                     HMTPVILLTDGYIANASELWPLPDFDHYEPIETHKPDPSTDPRHSVFSRDPKTEARLW
                     ATPGDKRFVYRVGGIEKDIHTGNISYEAQNHQEMTNMREAKVASVASFIPDQQVDSGT
                     DAGLAVVAWGSTYGTIYQAVRECVAEGLAVGHIHLRHLNPLPPNLPTLLAQFDRILVP
                     ELNNGQLATLLRDKLVLPVEQHNQVNGQPFAVADVKQAIARYAPKRIKEAG"
     gene            3266..4276
                     /locus_tag="AC16VR_09335"
     CDS             3266..4276
                     /locus_tag="AC16VR_09335"
                     /EC_number="1.2.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HIR6011399.1"
                     /translation="MAELTFKDYATDQEVRWCPGCGDYSILKAVQRALADAGAAKEQT
                     VFVSGIGCSSRLPYYVDTYGFHTIHGRAPAVATGVKLANPDLDVWVITGDGDALSIGS
                     NHLMHALRRNVDLNILLFNNEIYGLTKGQYSPTSRIGTRSPSSPKGSVDSPVQPGLFA
                     VGAGATFIARAVDVDQKVLPGILNAAREHKGAALVEILQNCIVYNKDVFEDVVAKKTA
                     ADTQVVVEHGHPLVFGKERDKGLRLNPQTLALEAVTLGENGVSEADLVVHDETNTALA
                     HMLLSLAPPNPVAMGIIHRRRAPAFDAAYWSSKPTGRSARVAELLRHGNVLDHRRSPQ
                     GR"
     gene            4440..5168
                     /locus_tag="AC16VR_09340"
     CDS             4440..5168
                     /locus_tag="AC16VR_09340"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR6011400.1"
                     /translation="MAKHEEIAESLTRDILSGQYRTGERLPSERDLSVRFEANRGAVR
                     EAMKKLEQLGIANIQPGGARVAPLEEASLDVIGHMLALGDLPDSKLVIHIIEVISALV
                     RLAVESALNTATDEDIERLREHNRPLFEEDLDDLAHMEARLELMGAIMSASDNLVCQL
                     IARSLLAQLTPRTEPLKAFAQFDTEAHADNARRLDEALAKRDADALRHVFTESTALTR
                     ESVLRSFEAYDAANRAPLTGVAAS"
     gene            5168..>5958
                     /locus_tag="AC16VR_09345"
     CDS             5168..>5958
                     /locus_tag="AC16VR_09345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6011401.1"
                     /translation="MKKLLVPGLVILASLFGAVTLMATAPELEPNAIEPLATTVRVVE
                     VSPQPVRLTVHSQGTVVPNMESELIPEVSGRVEWISPSLVNGGYFEQGDVLLRLEATD
                     YRSALTRAEAHLDRAAAEFEHAQYEYQRLKSLEVRQLASKSQMENSLRAFRVAEASLQ
                     EARAAMEQAMRDLERTALTAPFDGLVRREAVDVGQFVSRGSPVATVYASDQAEVRLPI
                     ADRQLAFLNLPVDHRGELPAEQQPRVTLLAEYAGRSLEWQGTIVRT"
BASE COUNT         1111 a         2001 c         1852 g          994 t
ORIGIN      
        1 cgaccagctt gtgatgcttc tgcagatagg ccagcctatc ctgcaactct tccttgatgt
       61 cttcgagcac cgcaagaatt cgctcgcgcg gcgtcacgta gtgagtcttg ggaaagaccg
      121 tgtagcgagg cacccggttg acgatctcgc ccgtcagagg atcgaagacg gagatgtttt
      181 cgatctcgtc gtcgaagagc tccacacgca cggcctcctt ctccgactcc gcgggaaata
      241 tgtccacgac gtcgccgcga acgcggtagg agccccgttg aaaagccatg tcgttgcgcg
      301 tgtactgcag ctccgccaag cgctgcagca cgtagcgctg gtccacgcga tccccacgat
      361 ccagatgcag aaccattcgc aggtaggact gagggtcacc cagcccgtag atggccgaga
      421 ccgacgccac gatgatggtg tcgcgacgct ccagcagcgc cttggtcgcc gacagccgca
      481 tctgctcgat gtgcgcgttg atcgacgagt ccttctcgat gtaagtgtcg gaagccggga
      541 cgtaggcctc cggctggtaa tagtcgtagt aggaaacaaa gtactccacg gcattgttcg
      601 gaaagaaatc ccgaaactcg ccgtagagct gtgccgccag agtcttgttg ggagccagga
      661 cgagcgtcgg ccgctgaacg gcctcgatga cgttggcgac ggtgtacgtc ttgcctgacc
      721 cggttacgcc gaggagcgtc tggtggctca ggccgtcgac gagaccctcg acgagaccgg
      781 cgatcgccgt cggctgatcg cccgcgggct catagtcgga aacgacctca aattccgcga
      841 cggccttgac cattgagcgc tattccggtt tgggttcctc ttcgccctct tcggccgaag
      901 ccggctcgcc cgcttcgtct tccgcagcaa cctcggccgc ggcggcttcc tcggcaggag
      961 cagccgcctc ggcgggttca gactcttccg cggccgactc ttcggcccca gtgacctctt
     1021 cggcggctgc ggcttccgcc gccggctcgc cggcgtcggc ttcgggctct tcgagcggcg
     1081 cggccggcag ggcggccgta atcagctcgt gctggacgag gatggcggcc ttcgcgtctc
     1141 cattgaccca ggccttcgtg gcatccttca cggcataacg ttcgtcgcgg cgctggtaaa
     1201 tggtgtactc gtcggttttg actacgactt tcacttcagg ctccagagtg tggcaagcgg
     1261 cgcactctaa gggatttgaa ccgcaaaatc atccggcgcc tgcaggggca gtcaggacat
     1321 aaccttatgt cccattggat ctccacttgc gcgcggaatt aagtacaatt ccgcaccttt
     1381 ttttgcgggc gggctccggg agcctcaaat ggtcgaggaa aaaaccagct acgttatccg
     1441 ctttgcgggg gactccggtg acggcatcca gcttcagggc cagcagttca ccacggcctc
     1501 cgctcttgcc ggccacgacc tggccaccat gcccgacttt ccggcggaga tccgcgcgcc
     1561 ggccggtacc cggtacggcg tgtccgcgtt tcagatccag ttcggcggcg accgggtcac
     1621 cacgccgggc gatgctcccg acgtcctcat tgccttcaac ccggccgcgc tggtggtcaa
     1681 cctgtcccat ctgaggccgg gcgcgctggt cgtcgtcgac cggcaggcct tcaccgacca
     1741 gcgcctgaag cgcgcggacc tgacgagcaa ccccctcgac gacggcaccc tcgatggttt
     1801 cgagctgctc gacatcgaca tctcggatct gacccaccag gctgtagacg ctctcgggct
     1861 gggcgccaag gactcactgc gctgcaagaa cttctgggca ctgggactcg tgctgtggat
     1921 gttcgatcag aaacgcgacg ccacggtctc ctggatcgag gcgaaattcg ccggtaacga
     1981 gcaagtgcga agcgccaacc tcgcagctct gaacgccggc cacgctttcg gcgagacggc
     2041 cgagctcggc ccgaagctgc gtcagcacgc cgcgcccgaa gcctcgctgg cgtcggccga
     2101 gtaccggacc ctcaccggcg ccgaggcgct ggccctgggc ctggtggccg ccggggagct
     2161 cgcggatacg gagctgatgt tctgctccta ccccatcacg ccggcctcct cgctgctgca
     2221 ccagctgacc gggctggagg atgccggggt ggcgaccttc caggccgaag acgagatcgc
     2281 ggcgatctgc tcggccatcg gcgcctcgta cgccggcaag ctgggcgtca cttcgagctc
     2341 cgggccggga atcgcgctga agaccgaggc catgggcctg gcggtggccg ccgagctgcc
     2401 gctgatcatc gtaaattccc aacgggcggg gccatccacg gggatgccca ccaaaaccga
     2461 gcagtccgac ctttaccagg ccgtgtacgg tcgcaacggg gacacgccgc tgccggtggt
     2521 cgcctcccgc tcccccgcgg attgcttcga taccgccatc gaggcggtgc gcatcgcgct
     2581 gcggcacatg acgccggtga ttcttctgac cgacggctac atcgccaacg cctccgagct
     2641 ttggcccctg ccggacttcg atcactacga gccgatagaa acgcacaaac cggatcccag
     2701 caccgatcca cgccattccg ttttcagccg cgatccgaag accgaggccc gtctgtgggc
     2761 aacgcccggg gacaaacgct tcgtctaccg ggtcggcggc atcgagaagg acatccacac
     2821 cggcaacatc tcctacgaag ctcagaatca tcaggaaatg accaacatgc gcgaggccaa
     2881 ggtggccagc gtcgccagct tcatccccga tcagcaggtg gattcgggaa ccgatgccgg
     2941 gctggccgta gtggcctggg gatccacgta cggaacgatc tatcaggcag tcagagagtg
     3001 cgtcgccgag gggctggccg tgggtcacat acatctgcgg cacttgaacc cgctgccgcc
     3061 gaacctgccg accctgctgg cgcagttcga ccggatcctc gttcccgagc tgaacaacgg
     3121 ccagctcgcg accctgctgc gagacaagct ggtcctcccg gtggagcaac acaatcaggt
     3181 caacgggcag ccgtttgccg tggcggacgt caagcaggcg atcgcgcgat acgcgcccaa
     3241 gcgcatcaag gaggcaggtt gaatcatggc ggagctgact ttcaaagact acgcgactga
     3301 ccaggaagtg cgctggtgcc cgggatgcgg tgactactcc atcctcaagg ccgtgcagcg
     3361 ggcgctggct gatgctggag ccgccaaaga gcagacggta ttcgtgtcgg gcatcggctg
     3421 ctcgtcacgc cttccctact acgtggacac ctacggtttt cacaccattc acggcagagc
     3481 gcccgccgtc gcgaccgggg tgaagctggc caaccccgac ctcgacgtct gggtgatcac
     3541 gggtgacggc gacgccctct ccattggcag caatcaccta atgcacgcgc ttcgccgcaa
     3601 cgtggacctg aatatcctgc tattcaacaa cgagatctac ggcctgacca aagggcagta
     3661 ctcgccaacg tcgcgcatcg gcacgagaag tccttccagc ccgaagggat ccgtggacag
     3721 ccccgtgcaa ccgggcttgt tcgccgtcgg tgcgggagcc accttcatcg ccagagccgt
     3781 ggacgtcgat cagaaggtat tgccgggaat cctcaacgcg gcgcgggaac acaaaggcgc
     3841 cgcgctggtg gagatcctcc agaactgcat cgtctacaac aaagacgtct tcgaggacgt
     3901 ggtcgccaag aaaacggcgg cggacactca ggtcgtcgtg gagcacggcc atccgctcgt
     3961 gttcggcaag gagcgcgaca aggggttgcg cctcaatccg cagaccctgg cgctggaagc
     4021 ggtgacgctc ggcgagaacg gcgtgagcga ggccgatctg gtggtccacg acgagaccaa
     4081 taccgccctg gcgcacatgc tgctgtccct ggcaccaccg aacccggtgg ccatgggcat
     4141 catccaccga cgtcgcgcgc cggccttcga cgccgcctac tggtcgagca aaccgaccgg
     4201 gcgcagcgcc cgggtcgccg agttgctgcg ccacggcaac gtcctggatc accgccgctc
     4261 tcctcagggc cgctgaccgc cgcccatccc caccgcggcc actggccgcg gtttcgcgaa
     4321 cgcgcgcaca tttcgttacc aaaggtaacg aaatgctgga catgcgaacc tgattgacta
     4381 aaatgccccg accatcatgg tcagaccaac atggtcgcac cagtcagaac aggaagaaca
     4441 tggcaaagca cgaggaaatc gccgagagcc tcaccaggga cattctgagc ggccagtacc
     4501 gcaccggcga gcggctgccc tcggaacggg atctttccgt gcgcttcgag gccaaccgcg
     4561 gcgccgtccg ggaggccatg aagaaactgg agcagctcgg catcgccaac atccagcccg
     4621 gcggcgcgcg ggtggcgccc ctcgaagaag ccagcctgga cgtgatcggc cacatgctgg
     4681 ccctgggcga ccttcctgac agcaagctgg tgattcacat cattgaagtc atttccgccc
     4741 ttgtcaggct tgcggtggag tcagcgctga atacggccac cgacgaagac atcgagcgcc
     4801 tgcgcgaaca caaccgaccg ctcttcgagg aggatctgga cgacctcgcc cacatggagg
     4861 ctcgcctgga gctgatgggc gcgatcatga gcgccagcga taatctcgta tgccagctca
     4921 tcgcccgcag cctgctggca cagctcacgc cgcgaaccga gccgcttaag gcattcgccc
     4981 agttcgacac ggaggcccat gcggacaacg cccggcgact agacgaagcc ctggccaaac
     5041 gtgacgccga cgctctgcgc cacgtgttca ccgagagcac tgcgctcacg cgcgaatccg
     5101 ttctacggtc cttcgaagcc tacgacgcgg ccaaccgcgc accgctcacg ggggtagccg
     5161 cgtcatgatg aagaagctgc tcgttccggg actcgtcatc ctggcatccc tcttcggagc
     5221 ggtaacgctg atggcaacgg cccccgagct ggagcccaac gccatagagc ccctggccac
     5281 cacggttcga gtggtggagg tgagtcccca gccggtccgc ctgaccgtgc actcccaggg
     5341 taccgtggta ccgaacatgg agagcgagct gatcccggaa gtgtccggac gcgtcgagtg
     5401 gatctcgccg tcactggtca atggaggtta cttcgagcag ggcgacgtgc ttctgcgcct
     5461 ggaagccacc gactaccgct cggcgttgac gagggcggaa gcccatctgg acagggccgc
     5521 ggcggagttc gagcacgcgc agtacgagta ccagcggctg aagagcctgg aggtacgcca
     5581 gctggcgagc aaatcccaga tggagaactc cctgcgcgcg ttcagggttg ccgaagcatc
     5641 cctccaggaa gcgcgcgccg ccatggagca ggcgatgcgc gacctcgaac gaaccgccct
     5701 caccgccccc ttcgacggcc tggttagacg cgaagccgtg gacgtcgggc aattcgtcag
     5761 ccgcggctct ccggtggcga ccgtttatgc cagcgaccag gcggaggtcc ggctgcccat
     5821 cgccgaccgc cagctggcgt tcctcaacct gcccgtggat caccgcggcg agcttcccgc
     5881 cgagcagcag ccgcgggtta cgctgcttgc cgagtacgcc ggccgcagcc tggaatggca
     5941 gggaaccatc gttcgaac
//
LOCUS       DBNHQR010000411         3208 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_165384, whole genome shotgun sequence.
ACCESSION   DBNHQR010000411 DBNHQR010000000
VERSION     DBNHQR010000411.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_165384"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="AC16VR_09350"
     CDS             complement(<1..376)
                     /locus_tag="AC16VR_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR6011402.1"
                     /translation="MTSPAYGNWHSPLQAAEIASGAIALRDLRSHGDTLYWLESRPAE
                     QGRTTLMALRPGERPTELTPEPFNVRTRVHEYGGGAYLPCAEGVFFANWSDQRIYLIA
                     NGAISALTRNDQSLRFADFCWDG"
     gene            complement(381..902)
                     /locus_tag="AC16VR_09355"
     CDS             complement(381..902)
                     /locus_tag="AC16VR_09355"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6011403.1"
                     /translation="MAERIYIRPVSRRDRSEFLHMTQRSQHVHEPWISPPLSDATFHS
                     YLKRTEQDDHEGLLVCLTDNDDIVGVININNIVRGSFLSASLGYYVSATQMGHGYMRE
                     GLELVKTYAFCQLGLHRLEANIQPENGRSIELVRSCGFQLEGLSRSFLFIAGSWRDHE
                     RWTAFHPRATLLP"
     gene            complement(895..1119)
                     /locus_tag="AC16VR_09360"
     CDS             complement(895..1119)
                     /locus_tag="AC16VR_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011404.1"
                     /translation="MRYLSRFALLCMGFALTTAGLMLWHAVGFGFHGIWFFDNDYRLH
                     PLHLLIVGLAMIPPALWEIFVLESRRRSRG"
     gene            complement(1116..2042)
                     /locus_tag="AC16VR_09365"
     CDS             complement(1116..2042)
                     /locus_tag="AC16VR_09365"
                     /inference="COORDINATES: protein motif:HMM:NF024919.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HIR6011405.1"
                     /translation="MILTERFADPDTGKRYPLRCGGPIGWRSAGDKHTAIIQLPPIPA
                     GAILVPSLSTAPSPERFQFVLRAASRLWALHEVPCRAAPQPSADTAVTTHVDCFHVHE
                     DLPRAELTVTVTGPSSPARYLLTVTARQLELERAPAPTRSAICKAPPFLSQMALGTSV
                     GPRVCSPACITMLLARHGHAADLKSVSESCFDAVTNLYGIWPLAIRAAAEQGSLGAVE
                     LFDDWEEPLSVLDAGMPFAASVRFGEGELPGAPLTRTAGHLVVVHGADPQDVHVNDPR
                     APEAASVPRRYPAVDFSRAWLRHRGAAYILLP"
     gene            2064..2315
                     /locus_tag="AC16VR_09370"
     CDS             2064..2315
                     /locus_tag="AC16VR_09370"
                     /inference="COORDINATES: protein motif:HMM:NF017574.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HIR6011406.1"
                     /translation="MNETGLILFSTDHCALCDEALGLLLSMPELAGISLRVVDVADDD
                     DLIATYGQRLPVLSYAGRELDWPFAATDLLRWIRQVEQP"
     gene            complement(2283..3101)
                     /locus_tag="AC16VR_09375"
     CDS             complement(2283..3101)
                     /locus_tag="AC16VR_09375"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR6011407.1"
                     /translation="MSERLTDIGVNLLNRQFQEDRQAVIDRAHRAGVTRMLITCTDLA
                     ESRLGQAFCQSNPEGFWCTAGVHPHHAKDVGPGWLEELAELAGQPCVRAVGETGLDFN
                     RNFSTPEQQLAVFDAQLKLAANTGKPVFVHDRDSDGKTFELLSRYREKLVGVVVHCFT
                     GTQTDLRHYLEAGFYIGITGWVCDPRRGGGLRDIVTMIPLDRLLIETDAPYLRPHNAP
                     VSPEGPARHKRRNEPALLGCVASQLAELYGIGEADIARASWQNASRLFDLPDPA"
BASE COUNT          583 a          981 c         1098 g          546 t
ORIGIN      
        1 ctccgtccca gcagaagtcg gcaaagcgca gggattggtc gtttcgagtg agcgcgctga
       61 tcgctccgtt ggcgatgagg taaatgcgtt gatccgacca gttggcaaag aacacgccct
      121 ccgcgcaggg caggtaggcg ccgccaccgt actcgtgcac gcgcgtgcga acattgaacg
      181 gctcgggggt gagctcggtg ggccgttctc ccggccgaag ggccatgagc gtggtgcgcc
      241 cctgctctgc cggccggctc tcgagccagt acagggtatc cccgtgagag cgcagatccc
      301 tcagagcgat ggctccggaa gcgatctcgg ctgcctgcaa aggggagtgc cagttgccgt
      361 aggcgggaga cgtcatgggc tcaaggaagc agcgtggcgc gcggatgaaa ggcggtccag
      421 cgctcgtggt cgcgccatga cccggcaatg aacagaaacg acctggacag gccctcgagc
      481 tggaaaccgc agctgcggac cagctcgatg gagcggccgt tctcgggctg gatgttcgct
      541 tccagacgat gcaggcccag ttggcagaag gcgtaggttt tgacgagttc caggccctcg
      601 cgcatgtagc cgtgacccat ttgcgtcgcg ctgacgtagt agcccaggga agcgctcaga
      661 aacgaacctc ggacgatgtt gttgatgttg atcaccccca cgatgtcatc gttgtccgtc
      721 aggcagacca acagcccctc gtgatcgtcc tgctcggtgc gcttgaggta gctgtgaaac
      781 gttgcgtcgc tcaggggtgg agatatccac ggctcgtgaa cgtgctggct gcgctgcgtc
      841 atatgcagaa actcgctgcg gtcccggcgc gagaccggtc ggatgtagat gcgttcagcc
      901 acggcttctt cgtcggctct ccagcacgaa gatctcccac agcgcgggcg ggatcatggc
      961 gagaccaacg atgagcaggt gcagcggatg caatcggtag tcgttgtcga aaaaccagat
     1021 gccatgaaag ccgaagccaa cggcgtgcca gagcatgagt ccagccgtcg tcagtgcgaa
     1081 gcccatgcac agcagcgcga agcgcgaaag atatctcatg gcagcaaaat atacgctgct
     1141 ccccggtgcc taagccaggc tctggagaaa tcgacggcag ggtagcggcg cggtacggaa
     1201 gccgcttccg gcgcccgcgg atcgttgacg tgaacgtcct gcggatcggc gccgtgcacg
     1261 accacgagat gaccggcggt gcgggtcaga ggcgctccgg gcagctcgcc ctcgccgaag
     1321 cggacgctcg ctgcgaatgg cattccagca tccagcacgc tcagcggctc ctcccagtcg
     1381 tcgaacaact ccaccgcacc aagggatcct tgctccgcag cggcgcggat ggccaggggc
     1441 cagattccgt aaaggttggt caccgcgtcg aagcaggatt cgctcacgga cttcagatct
     1501 gcagcgtgtc cgtgccgcgc gaggagcatg gtgatgcagg cgggcgagca cacgcgtggc
     1561 ccgacagacg tccccagtgc catctgagag agaaagggag gtgccttgca gatcgccgat
     1621 cgcgttggag caggcgcacg ttcgagctcg agctggcgcg ccgtgacggt caagagatag
     1681 cgagccgggc tcgaagggcc ggtgacggtg accgtcagct cggcgcgcgg caggtcctcg
     1741 tgaacgtgaa aacagtccac gtgcgtcgtg acggcggtgt cggccgacgg ctgcggcgcc
     1801 gcgcggcagg gaacctcgtg cagcgcccac agccgggaag cagcgcgaag cacgaactgg
     1861 aaccgctccg gggacggcgc cgtcgagagg ctgggtacga gaattgcgcc ggccggtatg
     1921 ggcggcagct ggatgatcgc ggtatgtttg tcaccggccg accgccatcc gatgggcccc
     1981 ccgcagcgaa gagggtaccg cttgccggtg tcagggtcgg cgaagcgttc tgtcagtatc
     2041 atgagttcca gcgattgacc aacatgaacg aaaccggcct gattctcttc tccacagacc
     2101 actgcgcgct atgcgacgag gcgctcgggc tgctgttaag tatgccagaa ctggccggga
     2161 tctccctgcg cgtcgtcgat gtggccgatg acgacgacct catcgccacc tatgggcagc
     2221 ggctgccggt gctgagctac gccgggcgcg agctggactg gccattcgcc gcgaccgacc
     2281 tgttacgctg gatccggcag gtcgaacagc cgtgatgcgt tctgccagct ggcccgggcg
     2341 atgtccgctt cgccaatccc gtagagctcg gcgagctgcg aggccacaca gcctagcaga
     2401 gcgggctcgt tgcgcctctt gtggcgcgcc ggcccttcag gagaaacggg cgcgttgtgc
     2461 ggacgcagat aaggcgcgtc ggtttcgatc agcaggcggt cgagggggat catggtgacg
     2521 atgtcgcgaa gaccgccccc ccgccgcggg tcgcaaaccc atccggtgat gccgatgtag
     2581 aagccggcct ccaggtagtg cctgagatcg gtttgggtgc cggtgaagca atggacgacc
     2641 acccccacca gcttctcccg gtatcggctg agaagctcga acgttttgcc gtcggagtct
     2701 cgatcgtgga cgaacacggg cttccccgtg tttgccgcca gcttgagttg cgcgtcgaat
     2761 acggccagct gctgctccgg cgtggagaag ttgcggttga agtccaggcc ggtttctcct
     2821 accgcccgca cgcagggttg ccccgcaagc tcggcgagct cctccagcca gcccggtccc
     2881 acgtccttgg cgtgatgcgg atggacgccg gccgtgcacc agaaaccctc ggggttcgac
     2941 tggcagaatg cctggccgag ccgggattcg gcgaggtcgg tgcaggtgat caacatccgg
     3001 gtcactcccg cccggtgagc cctgtcgatc acggcctggc ggtcctcctg gaactgacgg
     3061 ttgagcagat tgacgccgat gtcggtcagg cgctcgctca tgggatgctg gtctcgttga
     3121 cctggagcat gggaacgggc gtgcccagaa ggcgcccgta gacctgagcg aaggccagga
     3181 cgttcttgac gtagttgcgg gtttcacc
//
LOCUS       DBNHQR010000412         3700 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_418061, whole genome shotgun sequence.
ACCESSION   DBNHQR010000412 DBNHQR010000000
VERSION     DBNHQR010000412.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_418061"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1379
                     /locus_tag="AC16VR_09380"
     CDS             <1..1379
                     /locus_tag="AC16VR_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008172675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HIR6011408.1"
                     /translation="ARPGAGSLCRMLAKLSDEELNARKAAAELAIRSMGITFTVYTGE
                     GSGSIDREWPFDIIPRLLLKKEWDQISAGLTQRVKALNCFIDDVYHDQRVFADGVLPR
                     EYIEKSREFRGECVGIEPPHRIWSHVCGSDLVRDSDGRVYVLEDNLRVPSGVSYMLEN
                     REVMKRVFPELFEDYVIHPVDDYPARLASCLRTLTSVKSPQIAVLTPGIYNSAYFEHA
                     YLANAMAAQLVEGSDLVVGKDDYVYMKTIDGLVRVDVIYRRIDDLFLDPDVFKPDSML
                     GVPGIVRAWRAGNVALANAPGCGVADDKVIYTYVPDLVRYYLAEEPLLPNVPSYRCVD
                     KEQRDYVLDNLDELVVKPANESGGYGMLIGPKASRAERNRFARLIRANPRNYMAQPML
                     KLSTAPTLVDEHAEPRHLDLRPFILSGASSYVTMGGLTRVALRKGSTVVNSSQGGGSK
                     DTWVVDLE"
     gene            1376..2380
                     /locus_tag="AC16VR_09385"
     CDS             1376..2380
                     /locus_tag="AC16VR_09385"
                     /inference="COORDINATES: protein motif:HMM:NF016086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HIR6011409.1"
                     /translation="MSGHTLSSTAKRTYWLGRYLERTESTARLIAENGNLMIDLPARL
                     PHSWSPLISITGSGATFATLYEEPTERNVTRFLIGDLRNHGSILSSLGWARENVRTLR
                     GIIPRQAVEYIIELHLYARDHLSGALSRSRRAEGLTVVPALIQQIEGFMSANMLHDAH
                     WNFWRLGNFVERADMTTRIIDVGTDNPLQTADAPGPFTDLQWRSVLRSLNATQSYLTV
                     RQEPIHQSAVVGFLIGNRSLPRSLTYSLESLRNGLRTLPRSDRALRAVNRLRRFLQSS
                     EIENLEGPELHQFLDGCQVQLAEIHQQIDRTYFEFHPRRIPQRADRSADTQPTRSDST
                     "
     gene            2377..2688
                     /locus_tag="AC16VR_09390"
     CDS             2377..2688
                     /locus_tag="AC16VR_09390"
                     /inference="COORDINATES: protein motif:HMM:NF024809.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6011410.1"
                     /translation="MIRLYGMGRSNYFSIAKACLVERGMAFEEVATQPSAENAYLAKS
                     PMGKIPCIETERGFLSEAYAIVERIFGKDLLDSIPRARDLLALVGQQPSIQRVEAEKA
                     G"
     gene            complement(2698..>3700)
                     /locus_tag="AC16VR_09395"
     CDS             complement(2698..>3700)
                     /locus_tag="AC16VR_09395"
                     /inference="COORDINATES: protein motif:HMM:NF012686.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HIR6011411.1"
                     /translation="FKGGGHLFQQFLGIPYEGAVGLTLVIVMLYTSIGGFVSVVRTDV
                     VQGILMVIGSVTIFYFVTRAAGGVGVIGQLAERPGSEHVFTWSGGIPFAVLLGISLSG
                     SLKLIVEPRQVSRFFALKDQRSVRVGTWVALGGLTVIMACLFPVGFYAHFLLDGVQDT
                     DMIVPILVSDPNVFPGWVADFLIVAMMAAAMSSMDSVLLVSASVLYKDVVEVVHPAAH
                     PLTWTRIGVVGLALLAAGVALRPPGGIVEITIFSGSLYAVCFFPAILFGLHWRGGSAQ
                     AVLASMVSGVLVLVGWLLAGGREFAHEVFPAMLVSCLSYAWVANVTRPALEHWPPEV"
BASE COUNT          764 a         1248 c         1118 g          570 t
ORIGIN      
        1 gcgcccggcc gggcgccggc agcctctgcc gaatgcttgc caagctgagc gacgaagagc
       61 tcaacgcccg caaggcggca gcggagctgg ccatcagatc catggggatc actttcaccg
      121 tctataccgg agagggaagc ggttccatcg atcgtgagtg gccgttcgac atcatccccc
      181 gcctgctcct gaagaaggag tgggaccaga tctcggcagg gttgactcag cgcgtcaagg
      241 ccctgaactg cttcatcgac gacgtctacc acgaccagcg cgtgttcgcc gacggcgtgc
      301 ttcctcgaga atacatcgag aagtcccggg aatttcgagg ggaatgcgtc ggcatcgagc
      361 ccccgcaccg catatggtca cacgtctgcg gctccgatct ggtccgcgac tcggacggcc
      421 gcgtctacgt gctcgaggac aacctccgtg tgccttccgg cgtttcctac atgctggaaa
      481 accgggaagt catgaagcgc gtctttccgg agcttttcga agactacgtg atccaccccg
      541 tggacgacta tccagccagg ctcgcgagtt gtctgcgcac gcttacctcg gtgaaatccc
      601 cgcagatcgc cgtgctcacg cccggcatct acaactcggc ctacttcgag cacgcctacc
      661 tcgccaacgc catggccgcg cagctcgtgg agggctccga cctcgtcgtc ggcaaggacg
      721 actacgtgta catgaagacc atcgacggcc tggtccgcgt cgacgtcatc taccgtcgca
      781 tcgacgatct ctttctggat ccggacgtct tcaaaccgga ttccatgctc ggcgtccccg
      841 gcatcgtgcg cgcctggcgc gcgggcaacg tggcgcttgc caacgcgccc ggctgcggcg
      901 tggcagacga caaggtgatc tacacctacg tccccgacct cgtccgctac tacctggccg
      961 aggagccttt gctgcccaac gtgccttcct accggtgcgt ggacaaggag cagcgtgact
     1021 acgtgctcga caatctggac gagctggtgg tgaagcccgc caacgagtcc ggaggctacg
     1081 gcatgctgat cgggcccaag gcgtcacggg cggagcggaa ccggttcgcc aggcttattc
     1141 gcgcgaaccc gcgcaactac atggcccagc ccatgctcaa gctctccacc gcgcccacgc
     1201 tggtcgacga gcacgccgaa ccccggcatc tggacttgag acccttcatt ctctccggcg
     1261 ccagcagcta cgtgaccatg ggcggattga cccgggtcgc gctgcgcaag ggctcgaccg
     1321 tggtcaactc gtctcagggc ggcggcagta aagacacctg ggtggtggac ctggaatgag
     1381 cggccatacg ctgtcgagca cggcaaagcg cacctactgg ctgggacgct acctggagcg
     1441 caccgagagc accgcaaggc tgattgcgga gaatggcaac ctgatgatcg atctgcccgc
     1501 acggctcccc cacagctggt cgccgctgat cagcatcacc ggaagcggag cgacgtttgc
     1561 cacgctctac gaagagccca cggagcgcaa cgtcacccgc ttcctgatcg gcgacctgcg
     1621 caatcacggc agcatcctgt catcgctggg ctgggccagg gaaaacgtga ggaccctgcg
     1681 cgggatcatc ccgcggcagg ccgtggagta catcatcgag ctgcatctgt acgcgcgcga
     1741 ccacttgagc ggcgccctgt cacgctcccg tcgagccgaa gggctaaccg tggtccccgc
     1801 gctgatccag cagatcgaag gcttcatgtc cgcgaacatg ctgcacgatg cccactggaa
     1861 tttctggcgg ctcggcaact tcgtggagcg agccgacatg accacccgca tcatcgacgt
     1921 gggcaccgac aaccctttgc agaccgcgga cgcgccgggg ccgttcaccg acctgcaatg
     1981 gcgcagcgtg ctgcgctctc tgaacgccac gcaaagctac ctgaccgtac gccaggagcc
     2041 catccatcag agcgccgtgg tcggatttct catcggcaat cgctcgctgc cgcggtccct
     2101 gacctactcg ctggagagcc tgcgcaacgg tctgcgcacg ctgcctcgca gcgaccgcgc
     2161 cctgagggcc gtcaacaggc tgcgcaggtt cctgcagagc agcgagatcg agaacctcga
     2221 gggccctgaa cttcaccagt ttctggacgg ctgccaggta cagctggcag agatacacca
     2281 gcagatcgac cggacctact tcgagttcca tccacggcgc ataccccaac gggcggatcg
     2341 ttccgcagac actcaaccaa ccaggagcga cagcacatga tcaggctcta cggcatggga
     2401 cgcagcaact atttcagcat cgccaaggcg tgtctggtgg agaggggcat ggcgttcgag
     2461 gaagtcgcca ctcagccgtc tgcggagaac gcctacctcg ccaagagccc catgggcaag
     2521 atcccctgca tcgagacgga acggggcttc ctttccgagg catacgccat cgtcgaaaga
     2581 atcttcggca aggatctgct cgactcgata ccgcgggccc gtgacctgct ggcgctcgtt
     2641 ggccagcaac ccagcattca gcgcgtagag gccgagaaag cgggctgagc gacgacgtca
     2701 gacctcgggg ggccagtgct cgagggccgg gcgggtcacg ttggcgaccc acgcatagct
     2761 gagacacgag accagcatgg cggggaacac ctcgtgagcg aactcccttc cgccggcgag
     2821 cagccagccc accagcacga gcacgccgct gaccatggac gcaagcaccg cctgggctga
     2881 acctccgcgc cagtgcagac caaacagtat ggccgggaag aagcagacgg cgtagagact
     2941 gcccgaaaat atcgtgatct cgacgatgcc gcccggcggc cgcagcgcta ccccggcagc
     3001 cagcagcgca aggccgacca caccgatgcg cgtccaggtc agcggatggg cagccggatg
     3061 gacgacctcg accacatcct tgtagagaac cgaggcggaa accagcagaa cgctgtccat
     3121 ggaggacatg gcagccgcca tcatggcgac gatgagaaag tccgcgaccc acccaggaaa
     3181 cacattcggg tcgctgacca ggatcggaac gatcatgtcc gtgtcctgca ccccgtcgag
     3241 cagaaagtgg gcgtagaaac ccacgggaaa cagacaggcc atgatgacgg tcaggccgcc
     3301 cagcgcgacc cacgtcccca cccgcacgct gcgctgatct ttcagcgcga agaatcgcga
     3361 aacctggcgc ggctcgacga tcagcttcag agaacccgag agggaaatgc ctaacagaac
     3421 ggcgaacggg ataccgccgc tccaggtaaa aacgtgctca ctccccggcc gctcggccag
     3481 ctggccaatc acccccacac ccccggcagc cctggtgacg aagtagaaga tggtcaccga
     3541 cccgatgacc atcagaattc cctgaaccac gtcggtgcgc accaccgaga cgaagccgcc
     3601 gatggaggtg tagagcatga cgatgaccag cgtcagaccg acggctcctt cgtaggggat
     3661 gccgaggaac tgctgaaaca ggtgcccgcc ccctttgaag
//
LOCUS       DBNHQR010000413         4640 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1622280, whole genome shotgun sequence.
ACCESSION   DBNHQR010000413 DBNHQR010000000
VERSION     DBNHQR010000413.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1622280"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="AC16VR_09400"
     CDS             <1..589
                     /locus_tag="AC16VR_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011412.1"
                     /translation="IYGVFVYWYTSFQGPLSEAEIETYLAALPEGGGNDERLALWRAF
                     MESDTGDDFVMINVVDLRDRPLAMEGVSPDETAADVLARYTAPFLGAAIRSAAHPILY
                     GNAAAPALDLWGIDGAERWSNGGLVRYRSRRDLMDQVVRTSRLDIHEFKIAAMEKTIA
                     YPLDPWWHLGDPRLLLALVLGCIGFGLQARGARGR"
     gene            complement(599..1480)
                     /locus_tag="AC16VR_09405"
     CDS             complement(599..1480)
                     /locus_tag="AC16VR_09405"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6011413.1"
                     /translation="MARLAGKNIVVTGSSRGLGRAFAIAMAAEGASVVINGTNREALA
                     EVEKTISSAGGNVLAVPGSVTEETTCAQLVSGCVETFGSIDVMINNAGIVRDRTLLKM
                     TVEEWDDVLAVNLRGAFACTKHAALAMREAGGGHIVQVISASGLSGGFGQGNYAAAKA
                     GMMGLLRTAVLELSRFNIRNNALWPVAETDMTQVVFERAAAMSREQGAEPPTPAEMGF
                     GKPEEVAQGLVWLVSDGAAHFDGQCMSFNGRKTAIWRHPAEEHVSFHDEPWSFDELND
                     YYRDVKPLPIYRPVRAE"
     gene            complement(1566..2429)
                     /locus_tag="AC16VR_09410"
     CDS             complement(1566..2429)
                     /locus_tag="AC16VR_09410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6011414.1"
                     /translation="MKIGVFATFMSPLGTAEMIQDFGRRAEAAGLDSIWMGEHVVLFD
                     EMEFPYPGSRDGRIPVPEGGGLLDTVATFGLLAGSTSRLRFGTGICLVPQRNPIYTAK
                     EFATLDWLSGGRIDFGIGVGWCKEEVIACGYGWEDRGERCDEFLTLMRKLWSEPVVSY
                     SGKHVNVQGCRMDPKPIQKPHIPIIVGGHTPRAMRRAAEFGAGWYGFGITPDQTSAVL
                     TELDAALGAAGRSRDGFEIIITPASEDADSMAAFADLGVDRLILHLGSQRPEKLAARV
                     DQLASLVQAAA"
     gene            2529..3818
                     /locus_tag="AC16VR_09415"
     CDS             2529..3818
                     /locus_tag="AC16VR_09415"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6011415.1"
                     /translation="MSDPASYNLMEDETRECPFPYFNAIRHEQPVYFMEELGAWYVSR
                     YEDVRHVKKHPEIFSNNIFEHGGHRGAERNIAEEYKSEHGWRRVSTLQRTDPPVHSKY
                     RKLINHAFTVSRIRKMTGYVETVVNDLIDQFIDKGEVDLVWEFAIPLPCTVIADQLGV
                     PRDKIWRLKEWSDAMLAPGGGFVDEAAALKCGQLVVEAQKFFAEVMEARRAEPRDDII
                     TDLVQAKVVDEPGGPERPLDMFELQDLLDQLLTGGNETTTNAIGSAMMLLLQRPGLME
                     KMRDDQKLIRNFLEESLRFETPVLHLWRITTQDTELGGAFIPKGSMVAIGYASANRDE
                     DVFQDSEVFDIERQKAGAHLAFGSGPHHCPGAALARQEMFSAFTILLHRLDNIRLADP
                     GDRFLHVPSSFLRGLEHLNLKFDVRDRERYLPGASAY"
     gene            complement(3833..>4640)
                     /locus_tag="AC16VR_09420"
     CDS             complement(3833..>4640)
                     /locus_tag="AC16VR_09420"
                     /inference="COORDINATES: protein motif:HMM:NF006123.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6011416.1"
                     /translation="QKSIFITGGAGGMGMATGRLFAQHGWFVGLFDLDESALELARAA
                     FDDSQSLVRRLDVTSETDFADAMAAFSERTGGRMDVMFNNAGIAPGAWFDEMPLDTIR
                     QVIDINVMGVIIGIRAALPLLRATPGSLCMSTSSSVATYGHAMRAAYTASKFAVKGLT
                     EALSLEFERFGIRTADVLPGCIDTPMLRNALSARTGEPFDESLFETLPKEGAYRLVPV
                     SAIADAVWNAYHSDVIHWYVPEEVGEIDRLKGADFDAARAETRAFLFGEG"
BASE COUNT          781 a         1528 c         1526 g          805 t
ORIGIN      
        1 aatctatggg gtgttcgtct actggtacac gtcgttccag ggcccgctca gcgaggcgga
       61 gatcgaaacc tacctggccg cgctcccgga ggggggcggg aacgacgagc ggcttgcctt
      121 gtggcgcgcg ttcatggagt cggacacggg tgacgacttc gtcatgatca acgtcgtcga
      181 tctgcgcgat cgcccgcttg ctatggaggg ggtttcgccg gatgaaaccg ccgccgacgt
      241 cctggcccgg tacacggctc cgtttcttgg agctgccatc cgcagcgcag cgcacccgat
      301 cctctacggt aacgcggcgg cccccgcgtt ggatctatgg ggaatcgacg gcgcggagcg
      361 ctggagcaac ggcgggctcg tgcgctatcg cagccgccgg gatctcatgg atcaggtggt
      421 tcgaaccagt cgcctggaca tccacgaatt caagatcgcc gccatggaga agaccatcgc
      481 ctacccgctg gacccctggt ggcacctggg cgatccgcgt ctgctgctcg ccctggtgct
      541 cgggtgcatt ggcttcgggc tgcaggcgcg gggcgcccgg ggccgctgag cgatccgcct
      601 actcggcgcg caccggtcgg tagatgggta gcggtttgac gtcccggtag tagtcgttga
      661 gctcgtcgaa gctccagggc tcgtcgtgaa acgacacgtg ctcctccgcc gggtggcgcc
      721 agatggccgt cttgcggccg ttgaacgaca tgcactggcc gtcgaaatgg gcggccccgt
      781 cgctgaccag ccacacgagc ccctgggcga cttcctcagg cttgccgaag cccatctccg
      841 ccggtgtggg gggctcggcg ccctgctcgc gggacatggc ggccgcgcgc tcgaacacca
      901 cctgggtcat gtcggtctcc gccaccggcc acagcgcgtt gttgcggatg ttgaagcggg
      961 agagctccag cacggccgtg cgcagcaggc ccatcatgcc cgccttggcc gcggcgtagt
     1021 tgccctgacc gaacccgccg gagaggcccg acgcggagat gacctggacg atgtggccac
     1081 cgccggcctc gcgcatggcg agcgccgcgt gcttggtgca ggcaaacgcg ccgcgaaggt
     1141 tgacggccag cacgtcgtcc cactcctcga cggtcatctt caggagcgtc cggtcgcgca
     1201 cgatgccggc gttgttgatc atgacgtcga tgctcccgaa ggtttccacg cagccggaca
     1261 ccagctgtgc gcaggtggtc tcctcggtga cggatccggg gaccgccaga acgttgccgc
     1321 ccgcggagga tatggtcttc tcgacctcgg ccagggcctc ccgattggtg ccgttgatga
     1381 ccacgctggc tccttccgcg gccatggcga tggcgaaagc gcgccctaag cccctcgacg
     1441 agccggtgac gacgatgttt ttccctgcaa ggcgtgccat gagcaaatgt ctccagtctg
     1501 gttgaggatc cccccgcggc cccattgtgc cacgagagcg ctcaccaccg gccggcacgg
     1561 ccggttcagg cggccgcttg gaccagtgac gccagctgat ctacccgcgc ggcgagcttc
     1621 tccgggcgct gtgagcccag gtgcagaatc aggcgatcga cgcccaggtc ggcaaaggcg
     1681 gccatgctgt cggcgtcctc cgacgccggg gtgatgatga tctcgaagcc gtcccgtgac
     1741 cggcccgctg cccccagcgc cgcgtccagc tcggtcagca cggcagatgt ctgatccggg
     1801 gtgatcccga aaccatacca gcccgcgccg aactcggcgg cccggcgcat ggcgcgaggg
     1861 gtgtggccgc cgacgatgat ggggatgtgt ggtttctgga tcggcttggg gtccatccga
     1921 catccctgca cgttcacatg cttgccggag tagctgacca ccggctcgct ccacagcttg
     1981 cgcatgagcg tgaggaactc gtcgcatcgc tcgccccggt cttcccagcc gtacccgcag
     2041 gcaattacct cctccttgca ccatcccacc ccgatgccaa agtcgatccg tccgccgctc
     2101 agccagtcca gggtggcgaa ctcctttgcg gtgtagatgg ggttgcgctg ggggaccagg
     2161 cagataccag tgccgaagcg cagccgggaa gtgcttcccg ccagcagccc gaaggtggcg
     2221 acggtgtcga gcagcccgcc gccctcggga accgggatgc gaccgtcccg ggatcccggg
     2281 tagggaaact ccatctcgtc gaatagaacc acgtgctccc ccatccagat ggaatcgaga
     2341 cccgccgcct cggcccgacg gccgaagtcc tggatcatct ccgccgtgcc cagcggcgac
     2401 atgaaggttg cgaacactcc gatcttcata gcagttgcct ccctgttctg ttctacagat
     2461 tctaggggtt cgccgcccgc tttgctggta ggattcagcg gttgaatgtg tgtttggtgg
     2521 gagagcgcat gtcagacccg gcgagttaca acctgatgga ggacgagacg cgagagtgtc
     2581 cgtttcccta tttcaacgcg ataaggcacg agcagcccgt ctacttcatg gaagagctcg
     2641 gcgcgtggta cgtcagccgc tacgaagacg tccgccacgt gaagaagcac cccgagatct
     2701 tttcgaacaa cattttcgag cacggcggcc accgcggcgc ggagcggaac atcgccgaag
     2761 agtacaaaag cgagcatggc tggcggcgcg tgtccacgct gcagcggacg gatccgccgg
     2821 tgcattcgaa gtatcgcaag ctgatcaacc acgccttcac cgtcagccgg atccgcaaga
     2881 tgacgggata cgtggagacc gtggtcaacg atctcatcga tcagttcatc gacaaggggg
     2941 aggtggatct ggtctgggaa ttcgccatcc cgctgccctg cacggtgatc gccgaccagc
     3001 tcggcgttcc ccgtgacaag atctggcgcc tcaaggaatg gtccgacgcc atgctcgcac
     3061 ctggcggtgg gttcgtggac gaggcggctg cgctgaagtg cgggcagctg gtggtcgagg
     3121 cccagaagtt ctttgccgag gtcatggagg cgcgccgcgc cgagcctcgc gacgacatca
     3181 tcaccgatct cgtgcaggcc aaggttgtcg acgagcccgg tggtcccgag cgtccgctgg
     3241 acatgttcga gctccaggat ctgctcgatc agctcctgac gggcggcaac gagaccacca
     3301 ccaacgccat cgggtccgcc atgatgcttc tgcttcagcg gccggggctg atggagaaga
     3361 tgcgggacga ccagaagctt atccgcaact tcctcgaaga gtcgctgcgc tttgagacac
     3421 ccgtgctcca tctgtggcgg atcaccacgc aggataccga gcttggcggg gcgtttattc
     3481 ccaaaggatc catggtggcc atcggttacg cttcagcgaa tcgggacgag gacgttttcc
     3541 aggattccga ggtcttcgac atcgagcgtc agaaagcggg cgcccatctg gcgttcggct
     3601 ccggtccgca ccactgcccc ggcgcggccc tggccaggca ggagatgttc tcggcattca
     3661 ccatcctgct gcaccggctg gacaacatcc gcctggcgga tccgggcgac cgattcctgc
     3721 acgtccccag cagcttcctg cggggcctcg agcacctcaa cctgaaattc gacgtccgcg
     3781 accgggagcg ttatctgcca ggggcgtccg cttactagcg acccccgcgc gctcagccct
     3841 cgccgaacag gaaagcccgg gtctccgccc gggcggcgtc gaagtcggcg cccttgaggc
     3901 gatcaatctc gcccacctcc tcgggcacgt accagtggat cacgtcggag tggtaggcgt
     3961 tccagacggc gtccgcgatg gcggaaaccg gcacgagccg gtaggcgccc tccttgggga
     4021 gggtctcgaa cagggactcg tcgaagggct cgcccgttcg cgccgacagg gcattgcgca
     4081 gcatgggcgt gtcgatgcag cctggcagca cgtcggcggt gcggatgccg aagcgttcga
     4141 actccaggct gagcgcttcg gtcaggccct tcaccgcgaa cttggaggcc gtgtaggcgg
     4201 cgcgcatggc gtgtccgtaa gttgccaccg aggaggacgt gctcatgcac agcgagcccg
     4261 gcgtggctcg cagcaggggc agggcggcgc ggatgccgat gatcaccccc atgacgttga
     4321 tgtcgatgac ctgacgaatg gtatccagcg gcatctcgtc gaaccacgcc cccggcgcta
     4381 tccctgcgtt gttgaacatc acgtccatgc gcccccccgt gcgctcggag aacgcggcca
     4441 tcgcatcggc gaagtcggtc tcgctggtga cgtcgagccg ccggacgagg ctctgcgagt
     4501 cgtcgaaagc cgcccgcgca agctccaggg cgctttcgtc gaggtcgaag aggccgacga
     4561 accagccatg ctgggcgaag aggcgtccgg ttgccatgcc catgccgccg gccccgccgg
     4621 taatgaaaat cgatttctgc
//
LOCUS       DBNHQR010000414         2843 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_101963, whole genome shotgun sequence.
ACCESSION   DBNHQR010000414 DBNHQR010000000
VERSION     DBNHQR010000414.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_101963"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..2293)
                     /locus_tag="AC16VR_09425"
     CDS             complement(92..2293)
                     /locus_tag="AC16VR_09425"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6011417.1"
                     /translation="MKIFSRIKGPRLGTKIMLLGLTLLIVPWFSYRQLVEMERLLIQG
                     QSQAQLLTAEGISTLFNGREDLFNDLPVAVEDFENLYANPLQAAVRLDGRLDDWGSEI
                     PGKLMHFGSEKTGADADFELVLGERGGQLHVFMRVTDPDLIYRNPDYLRLDNADHVRL
                     SFIRADGEDGRILLTLPAPGLVTAYEMDADWRFAESGNPTNAVQGFMEPVDGGLQLEF
                     RLPLQLLGSSRYFGIAYVDVDDPALREIVHVTQTLPKAGLESFNLVVLRSPEVLNIIQ
                     GLGYSGARILVIDAQQRVRAETGSNRPIEETDPASPTFVLLRDGFEQVRPFLHWVSTG
                     EDYLPVKSRPEDSEGTADVAITTSLTGDPIALRRALSENNEIIIAAHPIVSRDAVIGT
                     VVVEQNIDDILSFQRSALEQVILLSIASLFAVFIALLAFAGRLAWRIRNLRQEASSAI
                     DQYGRLKISELHNEMKAGDEIGDLARSVSNMLSKLHQHNNFLENMPRTLRHEINNPLN
                     TLSTSLQNLAEENPEIQDSKYMESAKRGVNRIGSIVQNLADAANLEESLEAEELEVVD
                     IAQLLENYVANCRITHPTCEFVFRGPNRPVLSRVSDFRIEQLLDKIIDNAIDFHRSNS
                     PIVVQLDAYRDHLQITVANRGPILPAKAEKSLFDSMVSHRGQQNRLHFGLGLYVVRVI
                     ADHHGGFVRALNLADGSGVAIVVQLPTVETTEGTPGRQRDAEDIEPGVAAS"
     gene            complement(2309..>2843)
                     /locus_tag="AC16VR_09430"
     CDS             complement(2309..>2843)
                     /locus_tag="AC16VR_09430"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR6011418.1"
                     /translation="LGEEVDAGFQLCRDLLARAPSLPVIFLTERINEIDKISGLRLGA
                     WDYLPKPISLDYLAERISSLLRINEVRAEQQETESATAKRIGDLMLDQDALLVSWKGH
                     RIDLSGTEFRMLAKLVRMPGHAVSYETLMNATMQSLVTNNTINTHMRNIRKKFEKVDK
                     DFSSIKSEYGFGYRWSA"
BASE COUNT          476 a          862 c          970 g          535 t
ORIGIN      
        1 gcagcgttct ggctgcggtg gaagggctgg tggcccgggg cgacctcgcc tgcgacggcg
       61 cggtctctct cgacggcaac taccgactga cttagctcgc cgctaccccg ggctcgatat
      121 cctccgcgtc ccgctgacga cccggcgttc cctccgtcgt ctcgactgtg ggcagctgaa
      181 cgacgatggc gactccggag ccatcggcca ggttgagcgc ccgcacgaac ccgccgtggt
      241 ggtcggcaat gacccggacc acgtacaggc cgaggccgaa gtgcagccgg ttctgctgtc
      301 cccggtgact gaccatggag tcgaaaagcg acttttccgc cttggcggga aggatggggc
      361 cgcggttagc caccgtgatc tgcagatggt cacgataggc atcgagctga accacgatgg
      421 ggctgttgga gcggtgaaag tcgatggcgt tgtcgatgat cttgtcgagc agctgctcga
      481 tgcggaagtc ggacacccgg ctgagaaccg gacgattggg cccgcgaaac acgaactcgc
      541 acgtcgggtg ggtgatgcgg cagttcgcca cgtagttttc caggagctga gcgatgtcca
      601 ccacttcgag ctcctcggct tccagcgact cttcgagatt cgcagcgtcc gccagattct
      661 gcacgatgga gccgatgcgg ttgacgccgc gcttggcgct ctccatgtac ttgctgtcct
      721 gtatctcggg gttttcctcg gccaggttct gcagggacgt gctgagggtg ttcagcgggt
      781 tgttgatctc gtgccggagc gtgcggggca tgttctccag gaagttgttg tgctggtgca
      841 gcttggacag catgttggaa acgctgcgcg cgagatcgcc gatctcgtcg ccggccttca
      901 tctcgttgtg cagctcgctg atcttcagac gcccgtactg atcgatggcc gagcttgcct
      961 cctgacgcag gtttcggatg cgccatgcga ggcggccggc aaaggcgagc agggcgatga
     1021 agacggcaaa caggctggca atggacaaca ggatcacctg ctccagggcc gaccgctgga
     1081 aggagaggat gtcgtcgatg ttctgctcca ccaccacggt gccgatgacg gcgtctctgg
     1141 agacgatggg gtgcgcggcg atgatgatct cgttgttctc gctcagcgcc cggcgcagag
     1201 cgatggggtc gccggtgagc gaggtggtga tcgccacgtc cgccgtgcct tcggaatcct
     1261 ccgggcggct cttcacgggc aggtagtcct cgccggtgga gacccagtgc agaaagggcc
     1321 gcacctgctc gaagccgtcg cgcagcagga cgaacgtcgg gctcgcagga tcggtctcct
     1381 cgatggggcg gttgctgccg gtctccgcgc gaacccgctg ctgggcgtcg atgaccagga
     1441 tgcgggcgcc ggaatacccc aggccctgaa tgatgttcag cacctcgggc gagcgcagca
     1501 ccaccaggtt gaagctctcc agccccgcct tgggcagggt ctgggtgacg tggacgatct
     1561 cgcgcagggc cgggtcgtcc acgtccacgt aggcgatgcc gaagtagcgt gacgagccca
     1621 gaagctgcag cggcaggcgg aactccagct gcagcccgcc atccaccggc tccatgaatc
     1681 cctgaaccgc gttcgtcggg ttgcccgact cggcgaagcg ccagtcggcg tccatctcgt
     1741 aggcggtgac gagcccggga gctggcaaag tgagcagaat ccgcccgtcc tcgccatcgg
     1801 cgcggatgaa gctcaggcga acgtggtcgg cgttgtcgag ccgcaggtaa tcggggttgc
     1861 ggtagatcag atccgggtcg gtgacgcgca tgaagacgtg cagctgcccg ccgcgctcac
     1921 cgagcaccag ctcgaagtcc gcatcggccc ccgtcttctc gctgccgaag tgcatgagct
     1981 tgccggggat ctcgctgccc cagtcgtcaa gccggccgtc caggcgcacg gccgcctgga
     2041 gggggttggc gtaaaggttt tcgaagtctt ccaccgcaac gggcagatcg ttgaacagat
     2101 cctcccggcc gttgaagagg gtggagatgc cttcggcggt gagcagctgg gcctgtgact
     2161 ggccctgaat gagcagccgc tccatctcca ccagctggcg gtagctgaac cagggaacga
     2221 tgagcagggt gagcccgagc agcatgatct tggtgccgag ccggggaccc ttgatgcgac
     2281 tgaagatctt catgggcgtt ggcgagcgtc aggccgacca gcgatatccg aacccgtact
     2341 cgctcttgat gctcgagaag tccttgtcga ccttttcgaa cttcttgcgg atgttgcgca
     2401 tgtgggtgtt gatggtgttg ttggtgacga ggctctgcat ggtcgcgttc atgagcgttt
     2461 cgtagctcac ggcatggccc ggcatgcgaa cgagctttgc cagcatccgg aactcggtgc
     2521 cggacagatc gatgcggtga cctttccagg acaccagcag cgcgtcctgg tcgagcatga
     2581 ggtcgccgat ccgctttgcc gtggcgcttt ccgtctcctg ctgctcggcc cgcacctcgt
     2641 tgatgcgcag cagcgaggag atccgctcgg ccaggtagtc caggctgatg ggcttgggca
     2701 ggtagtccca ggcgcccaga cgaagccccg agatcttgtc gatctcgttg atccgctcgg
     2761 tgaggaagat gacgggcaac gatggcgcgc gggcgagcag gtcccggcaa agctgaaagc
     2821 cagcgtccac ctcctcgccg aga
//
LOCUS       DBNHQR010000415         5326 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1115661, whole genome shotgun sequence.
ACCESSION   DBNHQR010000415 DBNHQR010000000
VERSION     DBNHQR010000415.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5326)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5326
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1115661"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="AC16VR_09435"
     CDS             complement(<1..270)
                     /locus_tag="AC16VR_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011419.1"
                     /translation="MCRDHHRVADLVLLDGPQGGFRIEPAEDGLTPWADWLAGNSADD
                     PPDDMAPRPNLLYTSGTTGFPKGTPLPPTMFAGGDTVREHLANLAA"
     gene            256..1029
                     /locus_tag="AC16VR_09440"
     CDS             256..1029
                     /locus_tag="AC16VR_09440"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding enzyme"
                     /protein_id="HIR6011420.1"
                     /translation="MVPTHFVRLLALPEEIRNQYDVSSLIRVGHTGAKCPVDVKRAMI
                     EWWGPVFFDAYGASEVGTTCMITSQEWLEHPGSVGKAIPPFEAMILDDGGNPLGPNEE
                     GQLYFKDTTGRGIVYHNAPEKAAAAHIAPGVFTLGEIAYMDEDGYVYITDRFSDMVVS
                     GGVNIYPAESEHVIIEHPEVLDVACIGVPNQEMGEELKALVIPRDPATPPDPEDVMAW
                     CRERLSHYKCPRTVDIVDDLGRTTMGKINKRKLRAPYWE"
     gene            complement(1054..1758)
                     /locus_tag="AC16VR_09445"
     CDS             complement(1054..1758)
                     /locus_tag="AC16VR_09445"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6011421.1"
                     /translation="MATISQRANLMADASALSEDPKEQTWQQTKSALTRTAILEAAVL
                     CFYELGYASTTTENIANAAGVSRGAMLHHFPTRFDLIRAAVEHLNQKRLDWYAAEEEE
                     IQRGAEHTRIEEGIDTYWKQLNTPEFIVFHELNVAARTDPELAEVLVPALREFRQKSE
                     EASRQVFPDLALSEAWERTNFLSRFLLEGMAAAKMIEGPEVPDQQMLEWLKRELRRSY
                     QDVLNTVRRPGSPTDE"
     gene            1815..2630
                     /locus_tag="AC16VR_09450"
     CDS             1815..2630
                     /locus_tag="AC16VR_09450"
                     /inference="COORDINATES: protein motif:HMM:NF018038.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HIR6011422.1"
                     /translation="MRSILLVTKGHPFEREPFFQIFDVMDGVDWTHVEHPAAEALFNV
                     REAEAYDAFVMYDMPGIRFHPDRAPDFAEPREDFRRQFLELVDSGHGFVFLHHAVAGW
                     PAWDEFAEIVGGRFLYLPAELRGRSCPDSGYRHGVEHTVSVVAKHPVTEGIPGSFSIT
                     DELYLYEVFEDSVEPLLRSDFSFTRDNFYSAARVVRDQKMYDNEGWEHEPGSNLVGWT
                     RNRGNSRIVYLQCGDDPVAYANPHFQKLIRNAIFWVSEASARGRAGSGGRTGA"
     gene            2739..4058
                     /locus_tag="AC16VR_09455"
     CDS             2739..4058
                     /locus_tag="AC16VR_09455"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6011423.1"
                     /translation="MTVYRKIERFADPQTSRTPPQKEPDPDLGYDIIPKERYTSPEFM
                     QLEWDRMWTKVWLMGAWIGDLQNPGDYVVTEIGKESIVITRDENGDYNAFYNVCSHRG
                     NQVAYGKGGNTKTFRCSYHLWEYNLRGEIVNVPDQETFPQGVPCEKLAIVKLPCAAWG
                     AWVWFSLNPDVEPLEDFLGMIPEHLDPYHFEKMALVNDVTVEWDVNWKASVDAFNETY
                     HVAGTHPQLMWMLEDMDVQIDCYEKHNRYLIPFGCVSTHIEDGTPIAEALRVFMESYG
                     MDPDNFDGDGLEVRRGIQKWVRANAEVQGFDYSELNDDQLTDDYHYMIFPNITFNTHC
                     NSVMVFRQRPHPTDPNKMFYDVQNYSMMPAGTEWPERPPHRQWKHGEDTLGEVLDQDA
                     SNLPMVQRGMNSTGFRGLWISSQELRIRHFHKTLDDYLHRQPIKWMT"
     gene            4060..4404
                     /locus_tag="AC16VR_09460"
     CDS             4060..4404
                     /locus_tag="AC16VR_09460"
                     /inference="COORDINATES: protein motif:HMM:NF025178.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HIR6011424.1"
                     /translation="MTPVAPQNALDDGEMMACNVNGVDVLLCRVDGQYYAVSNRCSHA
                     RQVLSTGKLRGHEINCPLHGARFDIRNGHCLAAPASQPIKTFPVTLEGGKVHVTVTAD
                     DLPARPRFGPLN"
     gene            4450..5208
                     /locus_tag="AC16VR_09465"
     CDS             4450..5208
                     /locus_tag="AC16VR_09465"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF048554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6011425.1"
                     /translation="MDLQLKGSKALITGGTKGIGRAIANALADEGCNVAICARNAEEV
                     EAAVADLSAKGVTAFGQAVDVGDGDAFKAWIAASAEALGGIDCIVSNVSGGGAQGEDA
                     WRRHLEYDMLGAVRMVEGAMPYLEKSENASVVLISTTAALEKFMSAGPYNALKAALLN
                     YNGALSQDLAPKGIRVNAVSPGPIFIEGGAWDNIKQHMTAFYDATVADIPIGRMGTAE
                     EVAAQVAFLASPRAGFTTGTNIVIDGGLTKRIQF"
BASE COUNT         1013 a         1623 c         1739 g          951 t
ORIGIN      
        1 ggcagccagg ttcgccaggt gctcgcgaac cgtgtcgcca ccggcgaaca tggtcggcgg
       61 cagcggcgtg cctttgggga aacccgtggt tccggaggtg tagagcaggt tgggccgggg
      121 cgccatgtcg tccggcgggt cgtcagccga gttgcccgca agccagtcgg cccagggcgt
      181 gagcccgtcc tcggccggtt cgatgcggaa gccaccctgc gggccatcga ggagtaccag
      241 gtcggcaaca cggtgatggt cccgacacac ttcgtgcggc tgctcgccct gccggaggag
      301 atccggaacc agtacgacgt cagctccctg atcagggtcg ggcacacggg ggcgaaatgc
      361 ccggtggacg tcaagcgggc gatgatcgag tggtggggcc ccgtcttctt cgacgcctac
      421 ggcgcaagcg aagtcggcac cacttgcatg atcacctcgc aggagtggct ggagcatccc
      481 ggctctgtcg gcaaggccat cccgccgttc gaggccatga tcctggacga cggtggcaac
      541 cccctaggac ccaacgagga gggccagctc tacttcaagg acacgaccgg ccggggcatc
      601 gtctatcaca acgcgccgga gaaggcggct gcggctcaca tcgcccccgg cgtctttacc
      661 ctgggcgaga tcgcctacat ggacgaggac ggctacgtct acatcaccga ccggttcagc
      721 gacatggtgg tgtccggcgg ggtgaacatc tacccggcgg agtccgagca cgtgatcatc
      781 gagcatccgg aggtgctgga cgtggcgtgc atcggcgttc ccaaccagga gatgggcgag
      841 gagctcaaag cgctggtgat tccccgggat ccggccacgc cgcccgatcc ggaggacgtc
      901 atggcgtggt gccgggagcg gttgtcccac tacaagtgcc ctcgcaccgt ggacatcgtc
      961 gacgatctcg ggcgcaccac catgggcaag atcaacaagc gcaagctgcg ggcgccttac
     1021 tgggagtaga accgggatcg cccggagcct gggttactcg tcggtgggcg agccgggtcg
     1081 gcggacggtg ttcagcacgt cctggtagct gcgcctgagc tcgcgtttga gccattcgag
     1141 catctgctga tcgggaacct ccgggccctc gatcatcttc gcggccgcca tgccctccag
     1201 cagaaaccgg gagagaaagt tcgtccgttc ccaggcttcg gagagcgcca ggtcggggaa
     1261 gacctggcgg gaggcctcct ctgatttctg ccggaactcg cgaagcgccg gtaccagcac
     1321 ctcggcaagc tcgggatccg tgcgggcagc gacgttcagc tcgtgaaaga cgatgaactc
     1381 gggcgtgttc agctgtttcc agtaggtatc gatgccttcc tcgatcctgg tgtgctcggc
     1441 gccgcgctgg atctcctctt cctccgccgc gtaccagtcc aggcgcttct ggttgaggtg
     1501 ctcgaccgcg gctcggatca gatcgaagcg cgtggggaag tgatggagca tcgctccccg
     1561 cgacacgccg gccgcgttgg caatgttctc cgtcgtggtg ctggcgtatc cgagctcgta
     1621 gaagcagaga acggcggcct caagaatcgc ggtgcgcgtc agcgcgctct tggtctgctg
     1681 ccaggtctgt tcttttgggt cctcgctcag cgcgctggcg tctgccatga ggttggcccg
     1741 ctgtgatatc gttgccatgt tatgcgccgt gcccaagcgg agcaaccgcg cggggcagga
     1801 tgcatggggg gagcatgaga agcattctgc tggttacgaa gggtcatccc ttcgagaggg
     1861 aaccgttctt tcagatcttc gatgtcatgg acggtgtcga ctggacccac gtggagcatc
     1921 ccgccgcaga ggctctgttc aacgtccgcg aggccgaagc ctacgacgcc ttcgtgatgt
     1981 acgacatgcc cggcatccgt tttcatcccg accgggcgcc cgatttcgcg gagccccgag
     2041 aggatttccg ccgtcagttt ctggagctcg tcgacagcgg tcacgggttc gtttttctgc
     2101 accacgcggt ggccggatgg ccggcatggg acgagtttgc cgagatcgtc ggcgggcggt
     2161 ttctctatct gccggcagag ctgcggggcc ggtcctgtcc ggattccgga taccgtcacg
     2221 gcgtcgagca cacggtcagc gtggtggcga aacatccggt gaccgagggc attccgggct
     2281 cctttagcat tacggacgag ctgtatctct acgaggtgtt cgaggacagc gtggagcccc
     2341 ttctgcgaag cgacttctcg ttcacccggg acaacttcta ctccgccgca cgggtcgttc
     2401 gcgaccagaa gatgtacgac aacgaaggct gggagcacga gccgggcagc aacctggtgg
     2461 gctggacgcg caatcgcggc aacagccgca tcgtctacct gcagtgcgga gacgacccgg
     2521 tggcctacgc caacccccat tttcagaagc tgatccgcaa cgcgatcttc tgggtttcgg
     2581 aggcgtcggc tcggggccgc gccgggagcg gcggccgcac gggtgcctga acgggcatgc
     2641 gggggtgcaa accggcggcg gtggtggtac aaatcgcctg ttaaaacctg cacagttgac
     2701 tgttttcagc tggcagccaa gagaggagga ttaacggcat gacggtttat cgcaagatcg
     2761 agcggttcgc agacccgcag acttcgagga cgccccctca gaaggaacct gatcctgatc
     2821 tgggctacga cattattccg aaagagcggt acacgtcccc cgagttcatg cagctcgagt
     2881 gggatcggat gtggaccaag gtctggctca tgggtgcctg gatcggcgat ctgcagaatc
     2941 cgggcgacta cgtcgtcacc gagatcggca aggagagcat cgtcatcacc cgggacgaga
     3001 acggcgatta caacgcgttc tacaacgtct gctctcatcg cggtaaccag gtggcgtacg
     3061 gtaagggcgg caacaccaag acgttcaggt gctcctacca cctctgggag tacaacctgc
     3121 ggggcgagat cgtcaacgtt cccgaccagg agaccttccc tcagggcgtt ccctgcgaga
     3181 agctcgccat cgtcaagctc ccctgcgctg cgtggggtgc ctgggtatgg ttcagcctca
     3241 acccggacgt ggagccgctg gaagattttc tcggcatgat ccccgagcac ctggatccct
     3301 accacttcga gaagatggcg ctggtcaatg acgtcacggt ggagtgggac gtcaactgga
     3361 aagcttcggt ggatgcgttc aacgaaacct atcacgtggc cggaacccat ccgcagctca
     3421 tgtggatgct cgaagacatg gacgtgcaga tcgactgtta cgagaagcac aaccggtacc
     3481 tgatcccctt cggctgcgtg agcacgcaca tcgaggacgg cacgccgatc gcggaggcgc
     3541 tccgggtctt catggaaagc tacggaatgg atccggacaa cttcgacggc gacggtctcg
     3601 aggtgcggcg cggcatccag aaatgggtgc gtgcgaatgc cgaggtccag ggcttcgatt
     3661 actcggaact caacgacgat cagctgaccg acgactatca ctacatgatc ttcccgaaca
     3721 tcacgttcaa cacccattgc aacagcgtga tggtgttcag gcagcgtccg catcccacgg
     3781 acccgaacaa gatgttctac gacgtgcaga actactcgat gatgccggcc ggcacggagt
     3841 ggcccgagcg gccgccacat cgtcagtgga agcacggcga ggacaccctg ggagaggtgc
     3901 tcgatcagga cgccagcaac ctgcccatgg ttcagcgcgg catgaacagc acgggattcc
     3961 gcggtctgtg gatctccagc caggagcttc gcattcgtca tttccacaag acgctcgacg
     4021 actatctcca cagacagccg atcaagtgga tgacctaaaa tgacgccggt ggcccctcag
     4081 aacgccctcg atgatggcga gatgatggct tgcaacgtca acggcgttga cgtgctattg
     4141 tgccgggtgg acgggcagta ttacgctgtg tccaaccgct gcagtcacgc caggcaggtg
     4201 ctctcgacgg gcaagctgcg cgggcacgag atcaactgcc cgctgcatgg tgcccgtttc
     4261 gacatccgca acgggcactg ccttgcggcg ccggcttcgc agccgatcaa gacatttccg
     4321 gtgactttgg aagggggcaa agttcacgta acggttaccg ctgacgatct gccggcccgt
     4381 ccccgattcg ggccgctcaa ctaacgcagc tccagacagc tgggaaacga aaaagagagg
     4441 acactgacga tggatttaca actcaaagga tcaaaagcgc tgatcaccgg tgggaccaag
     4501 ggtattggcc gggcgatcgc gaacgccctg gccgacgagg gctgcaacgt ggccatctgc
     4561 gcacgcaacg ccgaagaggt cgaggctgcc gtggccgacc tgagcgccaa gggcgtcacc
     4621 gctttcggcc aggccgtcga cgtgggtgac ggagacgcgt tcaaggcctg gatcgccgcc
     4681 agcgccgaag cgctcggcgg catcgactgc atcgtctcca acgtcagtgg cggtggtgcg
     4741 cagggagagg atgcctggcg caggcacctc gagtacgaca tgctcggcgc ggtccggatg
     4801 gtcgagggcg ccatgcccta tctggaaaaa tccgagaacg ccagcgtggt tctcatctcg
     4861 accactgcgg ccctcgagaa attcatgagc gcgggtcctt acaacgctct gaaagcggcg
     4921 cttctgaact acaacggtgc tctgtcgcag gatctggcgc ccaagggcat tcgcgtcaac
     4981 gccgtgagcc ccggcccgat cttcatcgag ggcggtgcgt gggacaacat caagcagcac
     5041 atgaccgcgt tttacgatgc caccgtcgcc gacatcccca ttggacgcat gggcacggcg
     5101 gaggaagtcg cggcgcaggt cgcgtttctt gccagcccgc gggcgggatt caccaccggt
     5161 accaacatcg tcatagacgg cggcctgacg aaacgcatcc agttctgagg tgcgggcggt
     5221 cgcccgaagg gataccgcgg gccggcgttg ccgacccgcg gcgcctttcg ccgtctgata
     5281 tctgataggt agttaaagga cgctacagca acctgatctc tggggg
//
LOCUS       DBNHQR010000416         5440 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_228872, whole genome shotgun sequence.
ACCESSION   DBNHQR010000416 DBNHQR010000000
VERSION     DBNHQR010000416.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5440)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5440)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5440
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_228872"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..945)
                     /locus_tag="AC16VR_09470"
     CDS             complement(121..945)
                     /locus_tag="AC16VR_09470"
                     /inference="COORDINATES: protein motif:HMM:NF041278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmcJ/NvfI family oxidoreductase"
                     /protein_id="HIR6011426.1"
                     /translation="MSGACLQRYCETSLNYGDGDSARTVQVTIRNARTTPDLEFEECG
                     FSRLDHVSEVHDWSDAAHLADVHMAEVRELAQGFLGCDHALAYPPIVRSPASARAVAD
                     YAPIEFVHSDFTDDYLDMIRDPDRAYGAFLAPALETIGVRQDEIAKAERVVMLQFWRN
                     VGARRPDRPLALCDAASVPRSDLFSILVPEYGGQRLEFETFGVRCPKDPDRHRWYTYP
                     DLTAEEVIVFRTYDSRCAAEGRPFWTPHSAFRDPRAGTDPAQRESVEMRVLCFFGL"
     gene            complement(956..1642)
                     /locus_tag="AC16VR_09475"
     CDS             complement(956..1642)
                     /locus_tag="AC16VR_09475"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR6011427.1"
                     /translation="MQKLYTWFAVIGFGSVAASLMVGFRHDPAAPAVNYLLNAGLFLA
                     YMVVHYVMMTPGFKKLVSSNPAGSPAERRLYMVVAIASWIAVYLIHYPMPGPALALPG
                     WVTFLGGCAFMMSFLAFTEGTTFEGIRAFAGVPGHEQTHGASAEAPLQTEGSYASVRH
                     PMYRGACYMGLTSLLLHPNAAQLAWAGIMGLTFLTFIPIEEKQLLKGRGDEYREYMKQ
                     TPYRIFRGVW"
     gene            1776..3116
                     /locus_tag="AC16VR_09480"
     CDS             1776..3116
                     /locus_tag="AC16VR_09480"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6011428.1"
                     /translation="MTIEIVNHTPEPAVIANYSTARYVDRDYMEREWEHVWRRSWLLA
                     GLESDVQKPGDYFVFDMGREQILVTRTASGNVQGFYNVCQHRGNLLVAGERGHADSFR
                     CAYHAWTYDTEGSLKIIPYQERFARGVPCAERSLRKVHTDVWDGFVFVSLAEEPEPLL
                     DFLGPVVEMLAPYRFRNMVLVEDQTVHHKCNWKAVVDNFSELYHVDFLHPQHKRMVDC
                     CNDTVHLFPRGHTGLAVPGATVNPRFPIPDEPTDLQSAQLSSIGLDPADFRGRVMDVR
                     EAVQKRKREVGAEMGFDYSVFSEDQLSDVWQYNLFPNIIFSFTPEHCWVMRPRPHPTD
                     PQQCYFDKLSLLLFADPEINSKATSIVGPGRPEAISGTHLPDDYVRPERDVFDHQAII
                     SGEKTMTITIDQDIELLGGVQTGMASAGFDTVWLNDDEMRVQHFHNELDRLIGS"
     gene            complement(3160..3675)
                     /locus_tag="AC16VR_09485"
     CDS             complement(3160..3675)
                     /locus_tag="AC16VR_09485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6011429.1"
                     /translation="MSEKEYAEDYEDVTQYGLDEDKETELRQKQRECTFMWTNKQGEP
                     VGVIMSYLETDDGRLWLTGSEQRARFPAIRRDPRTCVCISSAGTDMGYGKTVTYKGIS
                     IIHDKSNRKIKDWFYPTFAKKIRAEQGDDRVQQFVEFLDSPRRVVVEFVPTKKIVYDG
                     AKMAAATPAIR"
     gene            complement(3761..4645)
                     /locus_tag="AC16VR_09490"
     CDS             complement(3761..4645)
                     /locus_tag="AC16VR_09490"
                     /inference="COORDINATES: protein motif:HMM:NF024743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HIR6011430.1"
                     /translation="MKVVATITLAAAFGLSSNASVAAALDERIDAAGAEVVEVVNRSG
                     SVTVTGGSDDVVVVSGSLGSNALGLDLDRDGDRIVVAVRYPEGDSGRNMASKLNISMP
                     RAAGLVVRSISADLEIGGIDGEQRLNTISGDIVTAVGSADVELRTVSGDMAITGRNEA
                     AVLKAASVSGDFGADAIAGQVDAATTSGDLQLLAGTLERARLASVSGDIEVRAQVASK
                     GSLVMESTSGDVAVVVEDAADISVEAETFSGSIDSCFDAETDGGFGPGESLRFRKGDG
                     DRSVRIMSFSGNVVVCDS"
     gene            complement(4642..5190)
                     /locus_tag="AC16VR_09495"
     CDS             complement(4642..5190)
                     /locus_tag="AC16VR_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011431.1"
                     /translation="MSDDRDDLEFLRRVRGLSGEVEPERDLWPEIRAALDEPGPRAKR
                     WRLPSALAASLLLGAVAASLLFRAGTAPEPAGVPAARPALLEVGLAPELMRSRAQLIA
                     SLDAELARMSPESQAAVAESLKEIEASLAKIHDELEHNPDSRLLLDLLVSTYLRQIAV
                     LGEMDGLARSVARSAQIERTET"
     gene            complement(5190..>5440)
                     /locus_tag="AC16VR_09500"
     CDS             complement(5190..>5440)
                     /locus_tag="AC16VR_09500"
                     /inference="COORDINATES: protein motif:HMM:NF019886.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR6011432.1"
                     /translation="QHEPEEAWNEQASETAGASKDLDLERAIAGLPEQARNVFVLCGV
                     YGYGHREAAGLLGIAEGTSKAHYHTARRKLREALAGAE"
BASE COUNT         1001 a         1820 c         1641 g          978 t
ORIGIN      
        1 ggcttcgagc aactcgaccc aggacaaggg atagatgttc ttcagccgga cgaaaccgtc
       61 ccgtgcatag gcctcgcgct cggcgtccga cacgtctctg accggcgagc cgtccacggc
      121 ttagaggccg aagaagcaga gcacgcgcat ctccacgctt tcgcgctgag cgggatccgt
      181 acccgcgcgc ggatctctga atgcggaatg aggagtccag aagggccggc cctcggccgc
      241 gcagcggctg tcgtaagtgc gaaacacgat gacctcttcg gcagtcaggt ccgggtacgt
      301 gtaccagcga tgccggtccg ggtccttcgg acagcgcacg ccgaaggttt cgaactccag
      361 ccgctggccg ccgtactccg gcaccaggat ggaaaacaga tccgagcggg gcacgctggc
      421 cgcgtcgcac agggcgagcg gtcggtccgg tcgtcgggcc cccacgttgc gccagaactg
      481 cagcatgacc acgcgttccg ctttcgcgat ctcatcctga cggacaccga tggtctccag
      541 agccggcgcg agaaaggcgc cgtaggctcg atccgggtcc cggatcatgt cgagatagtc
      601 gtcggtgaag tccgaatgaa cgaactcgat gggcgcgtag tcggccactg cgcgggcact
      661 ggcagggctg cggacgatcg gcggatacgc cagcgcgtga tcgcaaccga ggaacccctg
      721 cgccagttct cgcacttcgg ccatgtgaac gtcggcaagg tgggcagcgt cgctccagtc
      781 gtggacctcc gagacatgat ccagtcggga aaaaccgcac tcctcgaatt cgagatccgg
      841 cgtcgttctt gcattgcgga tcgtaacctg gaccgttcgg gccgaatcgc cgtcaccgta
      901 gttcagcgat gtttcgcagt agcgctgcag acaagcgccg gacatcgcgc ggcgcctacc
      961 agacgcctcg gaatatgcgg taaggcgtct gcttcatgta ctccctgtac tcatcgccac
     1021 gtcctttgag caactgcttc tcctcgatgg gtatgaacgt caggaacgtg agccccataa
     1081 tccccgccca ggccagctgc gccgcgttgg gatgaagcag cagggaggtc agccccatgt
     1141 aacacgcgcc ccggtacatt ggatgtctga cgctggcgta ggaaccctcc gtctgcagcg
     1201 gcgcttcggc agaagcgcca tgcgtctgct cgtgtcccgg tactcccgca aaggcccgaa
     1261 tcccttcgaa cgtggtccct tctgtgaacg cgagaaaaga catcatgaac gcgcaacctc
     1321 ctagaaacgt cacccagccc ggaagcgcca gcgcggggcc cggcatcgga tagtgaatca
     1381 gatacacggc gatccaggag gcgatggcca ccaccatgta gagcctgcgc tccgcggggc
     1441 ttcccgccgg attgctcgaa accagcttct tgaacccggg cgtcatcatc acgtagtgga
     1501 cgaccatgta ggccaagaac aagccggcgt tgagaaggta gttgacggcc ggagctgcgg
     1561 gatcatggcg aaaccccacc atgagagagg cggcgaccga accgaagcca atgaccgcaa
     1621 accaggtata cagcttctgc atgctctcat cccctcagaa aagagtttcc cgaatatagc
     1681 cgaaggcgcc gcgcagcgcc tccccgcaag ggaggcgttt ccagccgcga ccgcggctgg
     1741 catcattggg cgcaggagaa gccagcggat ccgtcatgac catcgagatc gtcaaccaca
     1801 cccccgaacc tgccgtcatc gccaactact cgacggcccg ctacgtggac cgcgactaca
     1861 tggagcggga gtgggagcac gtctggcgca ggtcctggct gcttgccggg ttggagagcg
     1921 acgtgcagaa gcccggcgac tatttcgtct tcgacatggg gcgcgagcag attctggtga
     1981 cccgaacggc ttccggcaac gtgcagggct tctacaacgt ctgtcagcat cgaggcaacc
     2041 tgctggtggc cggcgagcgc ggtcacgccg acagcttccg ctgcgcctac cacgcgtgga
     2101 cgtacgatac cgaaggctct ctgaagatca ttccctacca ggagcggttc gccagaggtg
     2161 tcccgtgcgc cgagcgatcg ctgcggaagg tgcacacgga cgtatgggac ggtttcgtgt
     2221 tcgtctccct cgccgaggag cctgagccgc tgctcgattt tctgggtccg gtggtggaga
     2281 tgctcgcgcc ttaccgcttt cgcaacatgg ttctggttga ggaccagacg gtacaccaca
     2341 agtgcaactg gaaggccgtc gtcgacaatt tcagcgagct ttaccacgtg gactttctgc
     2401 atccccagca caaacgaatg gtggactgct gcaacgacac ggtgcatctg tttccccgcg
     2461 ggcacacggg cctcgccgtg ccgggcgcga cggtgaaccc gcggttcccg atccccgacg
     2521 agccgacgga ccttcagtcc gcgcagctat cgagcatcgg ccttgatccg gccgatttcc
     2581 gaggaagggt gatggacgtg cgggaggcgg tgcagaagcg taagcgcgag gtgggcgcgg
     2641 agatgggttt cgactactcc gttttctcgg aagatcagct ctccgatgtc tggcagtaca
     2701 acctgttccc aaacatcatt ttctccttca cgccggagca ctgctgggta atgcgcccgc
     2761 ggccccaccc gaccgatccg cagcagtgct atttcgacaa gctctcactg ctgcttttcg
     2821 ccgatcccga gatcaactcc aaagccacgt cgatcgtggg tcccgggagg ccggaggcaa
     2881 tatccggcac gcacctgcct gacgactacg tccgcccgga gcgcgacgtg ttcgatcatc
     2941 aggccatcat cagcggcgag aagaccatga ccatcaccat cgatcaggac atcgaactgc
     3001 taggcggcgt tcagaccggt atggcgtctg cgggtttcga tacggtctgg ctgaacgacg
     3061 acgagatgcg cgtgcagcac tttcacaacg agctggaccg actcatcggc agctgacatc
     3121 gcccggcgtg ggccttccgg gggcatctcc gatggccgct taccgaatcg ccggggttgc
     3181 cgccgccatc ttggcaccgt cgtagacgat cttcttcgtt gggacgaact ccacgacgac
     3241 ccgccgggga ctatcgagga actccacgaa ctgctgaacg cggtcatcgc cctgttctgc
     3301 ccgaatcttc tttgcaaagg tcgggtagaa ccagtccttg atcttgcggt tgctcttgtc
     3361 gtggatgatc gagattccct tgtaggtcac cgtcttcccg taacccatgt ccgtcccggc
     3421 gctggagatg catacgcacg tcctgggatc gcgccggatg gcgggaaacc gtgcccgctg
     3481 ctccgacccc gtgagccaga gacggccatc gtcggtctcg agatagctca tgatcacgcc
     3541 tacaggctcg ccctgcttgt tggtccacat gaacgtgcac tcgcgctgct tctgccgaag
     3601 ctccgtctcc ttgtcctcgt ccagcccgta ctgcgtgacg tcctcgtaat cctccgcgta
     3661 ttccttttcc gacatggctg cgcttccccg gtgataaagc ttcgagtcta gcccgtcacg
     3721 gtcccggcgc aaacaagctc gagcccacct gcggatcggc tcaggagtcg cagaccacca
     3781 cgttgccgct gaaactcatg attcgtacgc tgcgatcgcc gtcgcccttt ctgaaacgca
     3841 gcgactcgcc cgggccgaag cccccgtcgg tctcggcatc gaagcagctg tcgatggagc
     3901 cgctgaaggt ctcggcctct acggagatgt cagctgcatc ctcaaccacg acggcaacgt
     3961 cgccgctggt cgactccatg acgaggctgc ctttcgacgc aacctgggcg cgaacctcga
     4021 tgtcgccgct gaccgaggca agacgtgccc gctccagcgt tccggccagc agctggagat
     4081 cgccgctcgt cgtggccgca tccacctgcc cggcaatggc gtcggcgccg aaatcaccgc
     4141 tcacgctcgc tgccttgagc acggcggcct cgttccggcc agtgatcgcc atgtcgcccg
     4201 agaccgtcct caattccaca tccgcgctcc cgacagcggt gacgatgtct cccgagatcg
     4261 tgttgaggcg ttgctctccg tcaatcccgc cgatctccag atcggcgctg atcgatcgca
     4321 cgacgagacc ggccgccctc ggcatcgaga tgttcaactt gctcgccatg ttcctgcccg
     4381 agtcaccctc cgggtatcga acggcgacga cgatccgatc gccgtcccga tccagatcca
     4441 ggcccagggc gttgctgcca agggagcccg agaccaccac cacgtcatcg gagccacccg
     4501 taacggtgac cgatccagac cggttgacga cctcgacgac ctcggcgccg gctgcgtcga
     4561 tacgttcgtc cagagcggcc gccactgacg cgtttgagct cagaccgaac gcggcggcca
     4621 atgtgatcgt tgcgacgact ttcatgtttc tgtcctctca atctgggccg agcgggccac
     4681 ggagcgggca agcccgtcca tttcgcccag cacggctatc tgccgaagat aggtggagac
     4741 gagcaggtcg agcagcagtc ggctatcggg gttgtgctcg agctcgtcgt gaattttcgc
     4801 aagagacgct tcgatctcct tgaggctctc cgcgaccgcg gcctgggact ccggcgacat
     4861 ccgcgccagc tccgcatcca gggacgcgat cagctgcgcc cgggaccgca tgagctctgg
     4921 cgcgagcccg acctccagaa gtgccggacg ggcagccggc acgccggccg gctcgggagc
     4981 cgtcccggcg cggaatagca gcgacgcggc aacagcgcct agaagcagcg acgcggcaag
     5041 cgccgaaggc agacgccagc gcttcgcgcg gggccccggc tcatccagcg ctgcgcggat
     5101 ttccggccag agatcccgct cgggctcgac ctccccggaa agcccgcgaa cgcgccgcag
     5161 gaattccagg tcgtcgcgat cgtcactcat cattcagctc ccgccagcgc ttctctcaac
     5221 ttgcgcctgg ccgtgtggta atgcgccttg ctcgtgcctt ccgcgatacc cagcaggcca
     5281 gccgcttcac gatggccgta cccgtagacg ccgcatagca cgaatacgtt gcgggcctgc
     5341 tccggcaagc cggcgattgc ccgttccaga tccagatctt tcgacgctcc cgcagtttcc
     5401 gacgcctgct cgttccaggc ttcctccggt tcgtgctgat
//
LOCUS       DBNHQR010000417         1095 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_799814, whole genome shotgun sequence.
ACCESSION   DBNHQR010000417 DBNHQR010000000
VERSION     DBNHQR010000417.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_799814"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..809)
                     /locus_tag="AC16VR_09505"
     CDS             complement(60..809)
                     /locus_tag="AC16VR_09505"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6011433.1"
                     /translation="MSDAFRLDGQVAVVTGAGKGIGRGIALCLAQAGADVVVSARTLS
                     DVQGVAAEIEKLGRRALAVSADVTSAEQLETLVVAAQELGGLDIWVSNAGGLPDATPR
                     YLTRTPEDRWDAQIDLNLKAVWQGAVVAAQNMDRGVIINISSRSAQGPSFKNGPYGAS
                     KAAVNSLTQTLSLELAPNIRVNAVAPGPIPTENFNESTNFPDDKPIEQIIGVPLGRLG
                     TPEDIGNAVVFMASPAAAWITGQCLYVTGGL"
     gene            867..>1095
                     /locus_tag="AC16VR_09510"
     CDS             867..>1095
                     /locus_tag="AC16VR_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011434.1"
                     /translation="MRHALGAPRQGQEQTNMSYADGRLINDADSHLMELPDFLTAHAD
                     PGMRGRLPELNPGGQFDMSERTHQRGHPPEVV"
BASE COUNT          188 a          385 c          328 g          194 t
ORIGIN      
        1 ccaggcctcg taaggcgggg cccggtcctc tccttgctcc ggggaagacg ggcgatcgcc
       61 taaagcccgc cggtcacgta gaggcactgc cccgttatcc aggccgccgc cggcgaggcc
      121 atgaacacga cggcattgcc gatgtcctcg ggcgtgccga gccgtcccag gggcaccccg
      181 atgatctgct cgatgggttt gtcgtccggg aagttcgtgg attcgttgaa gttctccgtc
      241 gggatcggtc cgggcgctac ggcgttcacg cggatgttcg gcgccagctc cagcgaaagc
      301 gtctgggtaa ggctgttcac ggccgccttg gaggcaccgt aaggcccgtt cttgaaagaa
      361 gggccctgag cggatcgtga ggatatgttg atgatgacac cgcgatccat gttctgcgcc
      421 gcgacgacgg caccctgcca cacggctttg agattgaggt cgatctgcgc atcccagcgg
      481 tcctccggcg tccgagtcag gtatcgcgga gtcgcgtctg gcagaccgcc cgcattgctg
      541 acccagatgt cgagcccgcc cagctcctgt gccgccacca ccagcgtctc gagctgctcc
      601 gcgctggtga cgtccgccga tacggccagc gcccgacgcc cgagcttttc gatctctgca
      661 gccaccccct gaacgtcgct cagcgtgcgg gccgaaacca ccacgtctgc ccctgcttgt
      721 gcaaggcaaa gcgctattcc ccggccgatt cccttaccag cccccgtaac caccgccacc
      781 tggccgtcca gtcgaaacgc gtcggacatc gtcacaccct ctctttgaaa gcttcgtact
      841 atactaaacc cttcccggca tgattcatgc ggcacgcact aggcgcgccg cgccagggtc
      901 aggagcagac caacatgagc tacgccgatg ggcgtctgat caacgatgcc gacagccacc
      961 tgatggagct gccggacttc ctcactgccc atgcggaccc gggcatgcgt ggccgtcttc
     1021 ccgagctcaa tccgggaggc cagttcgaca tgagcgagcg aacccaccag cgcggtcacc
     1081 cacccgaggt cgtcg
//
LOCUS       DBNHQR010000418         3764 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_989614, whole genome shotgun sequence.
ACCESSION   DBNHQR010000418 DBNHQR010000000
VERSION     DBNHQR010000418.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_989614"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1798
                     /locus_tag="AC16VR_09515"
     CDS             <1..1798
                     /locus_tag="AC16VR_09515"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HIR6011435.1"
                     /translation="LVPAFEIFNRYQERLTDRLEFLLEELALGLKQIDFTAEDQIEIE
                     RENAPWPANEAELDDLWRKRLKAAVLSMKLNGKELDEIQTLLTKRYTNRLKQSRQTKS
                     EDAFQLYVNAFATTYDPHTQYFSPRTSQNFNINMSLSLEGIGAVLRSEDEYTSIVELV
                     PAGPADKSGLVKPADRIVSVGQGDTGPLIDVVGWRLDDVVELIRGPKGSQVRLEIIPG
                     DSEDDTSRVISITRNTVKLEEQAAQKKLLTMERNGQTQKIGVIEIPTFYVDFKAIQQG
                     DPNYKSTTRDVRLLIEELKAEGIDGLVVDLRNNGGGALQEADSLTGLFIKSGPTVQVK
                     SASRRPLVYSDDDNRAVWDGPLAVLVNRLSASASEIFAGAIQDYERGIIIGSQTFGKG
                     TVQTLVPLNRGQLKITAAKYYRVSGQSTQHQGIIPDIEFPEIYDAERIGESALDDAMP
                     WDMIQPAVYPRSHQIQPVLAELNSKHEERAGGDPDFQYFEALAQRSRENNRRTHVSLN
                     EKERVVQKKDEDTWRLDLENQLRVAKGEEPLESIDDLDDREADEDEAEDPFGDPMIRE
                     SANILLDYIGLTRQIAYVEDLDKAAGPATAIQ"
     gene            1930..3762
                     /locus_tag="AC16VR_09520"
     CDS             1930..3762
                     /locus_tag="AC16VR_09520"
                     /inference="COORDINATES: protein motif:HMM:NF015557.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR6011436.1"
                     /translation="MPEVAVESSLHAINAHAVAVMLLTVSALYLFTRERIPMEVTSVG
                     LLAILAVGFSIFPFPGIEPTDFFHGLGHEALVAVCALMVVGQGLVRTGALEPVGRLLG
                     KIWSRAPFLSFLITLIVGAVLSAFVNNTPIVVLLLPILISVCLRTSSSPSGILLPMGF
                     ATLVGGMATTIGTSTNLLVVSVANDLGLERFGMFDFALPAAIAGCVALLYLWLIAPRM
                     LPKRTIGLEDSAPRLFEAGLHLGDNSPAVDRTLAEAKAMTGGDMQVIRIRRGDTYLVP
                     LPDANLRAGDILRVRDTPQRLKSYEDVLKARLYSGDHEVDEDHPLSAGNQMLAEVAVV
                     RGSTLDRTNLRFARFLSRYQLAALALHRAGKSVERPIEEIEDVILQQGDILLVQGARD
                     QIAALKRSTEFLVLDASVALPHTSKAPLALTIVAGVVAAAAFGIMPIAVSAMAGAALL
                     LLTRCLNLGAAIRAISPSVFFIVVASLALGQALIATGATDYVTSLFLSATRGASPTVI
                     LSALMLLLAVLTNMISNNAAAVIGTPIGIGIAAQLGLPAEPFVLAVLFGANMSYATPM
                     AYKTNLLVMSAGNYSFSDFVRVGVPLTIIMWLTLTWVLSSMYGL"
BASE COUNT          693 a         1247 c         1169 g          655 t
ORIGIN      
        1 cctcgtgccc gccttcgaga tcttcaaccg gtaccaggag cgcctgaccg accggctgga
       61 gttcctgctg gaagagctgg cgctgggtct gaagcagatc gacttcaccg ccgaggacca
      121 gatcgagatc gagcgcgaga acgccccgtg gcccgcgaac gaggctgagc tggatgatct
      181 gtggcgcaag cggctaaaag cagcggtgct cagcatgaag ctcaacggca aagagctcga
      241 cgagatccag accctgctga ccaagcgcta caccaaccgg ctcaagcaga gccggcagac
      301 caagagcgaa gatgcgtttc agctctacgt gaacgccttc gcgaccacct acgacccgca
      361 cacccagtac ttctcgccgc gaacgtcgca gaacttcaac atcaacatgt cgctttccct
      421 ggaaggcatc ggcgccgttc tgcgctcgga ggacgagtac acgtccatcg tcgagctcgt
      481 ccccgccggg cccgcggaca aatccgggct ggtcaagccg gccgaccgga tcgtcagcgt
      541 gggccagggc gataccggac cgctgatcga cgtggtgggc tggcggctcg acgacgtcgt
      601 ggagctcatc cgtggcccca agggctccca ggtgaggctg gagatcatcc ccggcgactc
      661 ggaggacgac accagccgcg tgatcagcat cacgcggaac accgtgaagc tcgaggagca
      721 ggccgcccag aagaagctgc tcaccatgga gcgcaacggc cagacccaga agataggcgt
      781 catcgagatc ccgacgttct atgtcgactt caaggccatt cagcagggcg atcccaacta
      841 caagagcacc acgcgagacg tgcggctgct gatcgaggag ctcaaggcgg agggcataga
      901 cggcctcgtc gtcgatcttc gcaacaacgg cggtggcgcc ctgcaggaag cggattcgct
      961 caccgggctg ttcatcaagt ccgggcccac cgtgcaggtc aaatccgcct cgcgacggcc
     1021 cctggtctat tcggacgacg acaaccgggc cgtgtgggac ggccccctcg cggtgctggt
     1081 caatcgtctc tccgcttcgg cctcggagat cttcgcgggc gccatacagg actacgagcg
     1141 cggcatcatc atcggcagcc agaccttcgg caaaggcacg gtgcagaccc tggtcccctt
     1201 gaaccggggt cagctgaaga tcaccgcggc caagtactat cgggtgtccg gccagagcac
     1261 ccagcaccag ggcatcattc ccgacatcga gttcccggag atatacgacg cggagcgtat
     1321 cggcgaaagc gccctcgatg acgccatgcc ctgggacatg atccagcccg ccgtgtaccc
     1381 gcgatcacat cagattcagc cggtccttgc ggagctcaac tccaagcacg aagagcgtgc
     1441 gggcggcgat cctgacttcc agtacttcga agccctcgcc cagcgcagcc gcgagaacaa
     1501 ccgtcgcacc cacgtctccc tgaacgagaa agagcgcgtg gtgcagaaga aggacgagga
     1561 cacgtggcgc ctggatctgg agaatcagct ccgggtcgcc aaaggagaag agccgctgga
     1621 atccatcgac gacctggacg atcgggaggc ggacgaggac gaagcggagg atcctttcgg
     1681 cgatcccatg attcgcgaga gcgccaacat tctgctcgac tacatcggcc tcacccggca
     1741 gatcgcctac gtggaagacc tcgacaaggc tgccgggccc gcgacggcca tccagtagaa
     1801 aatcgccggg cgacccgacg ctccgggcca atattgagag atttctgcag gctttgaccc
     1861 cagaatagac gtacacttct ccgaccttct cgtggacgtc atctaagttg ctttcttgtc
     1921 agacgcacga tgcctgaagt ggctgtagaa agcagcctgc atgcgatcaa cgctcacgcg
     1981 gtcgccgtta tgctgctcac ggtcagcgcg ctctatctct tcacccgcga gcgcataccc
     2041 atggaggtga ccagcgtcgg tctgctcgcc atcctcgcag ttggattctc catttttccc
     2101 ttcccgggca ttgagcccac cgatttcttt cacgggctcg gccacgaagc gctggtggcc
     2161 gtctgcgccc tgatggtggt cggccagggc ctggtccgga caggggcgct cgagcccgtg
     2221 ggtcgattgc tcggcaagat ctggagccgc gctccgttcc tgtcgtttct gatcacgctc
     2281 atcgtcggcg ccgtgctgtc ggcgttcgtg aacaacacgc ccatcgtggt tctgctgctg
     2341 cccattctca tcagcgtctg cctgcgcacg tcatcgtcgc catccggcat tctccttccc
     2401 atgggtttcg ccaccctggt gggcggcatg gcgacgacca tcggcacgtc cacgaacctg
     2461 ctggtggtca gcgtggcgaa cgacctgggc ctggagcgtt tcgggatgtt cgacttcgcg
     2521 ctgcccgcgg ccattgcggg ctgcgtagcc ctgctttatc tgtggctgat cgccccccga
     2581 atgctgccga agcggaccat cggcctggag gactcggcgc cccggctatt cgaggccgga
     2641 ctgcatctgg gagacaactc gcccgcggtg gaccgcacgc tggccgaggc gaaggcgatg
     2701 accggcggcg acatgcaggt cattcgaatc cgccggggcg acacgtatct cgtgcctctg
     2761 cccgacgcca acctgcgcgc gggagacatt ctgcgggtca gggatacgcc ccagcggctc
     2821 aagtcctacg aggacgtgct gaaagcacgg ctctactccg gcgatcacga ggtggacgaa
     2881 gaccacccgc tttcagccgg aaaccagatg ctggccgagg tcgcggtggt tcgcgggtcg
     2941 accctggacc gcaccaacct gagattcgcc cgcttcctgt cgcgctacca gctggccgcg
     3001 ctcgccctgc accgggctgg aaaatcagtg gagcggccca tcgaggagat cgaggacgtg
     3061 atccttcagc agggagacat cctgctggtc cagggcgcac gggatcagat cgcggcctta
     3121 aagcgcagca ccgagtttct cgtcctggac gcgtcggtcg ctctgccgca cacgtccaag
     3181 gccccgctgg cgctgaccat cgtcgccggg gtggtcgccg cggccgcctt cgggatcatg
     3241 ccgatcgccg tgagcgccat ggcgggtgcg gcgctgttgc tgctcacgcg gtgtctgaat
     3301 ctgggggcgg ccatccgagc catcagcccc tcggtgttct tcatcgtcgt ggcaagcctg
     3361 gcgctcggcc aggccctcat cgccaccggg gcgacagact acgtgacgtc gcttttcttg
     3421 agcgcgaccc ggggggcatc gccgacggtc atcctgtccg cgctgatgct tctgctggcg
     3481 gtgctcacca acatgatctc gaacaacgcg gcagccgtca tcggcacgcc cattgggatc
     3541 gggatcgccg cgcagctggg gctaccggcg gagcccttcg tccttgccgt gctcttcggc
     3601 gcgaacatga gctatgccac gccgatggcc tacaagacca acctgctcgt catgagcgcc
     3661 ggcaactaca gcttctccga cttcgtccgg gtgggggtac cgctgaccat catcatgtgg
     3721 ctcacgctca cctgggtatt gagcagcatg tacgggttgt gagc
//
LOCUS       DBNHQR010000419         5696 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1053155, whole genome shotgun sequence.
ACCESSION   DBNHQR010000419 DBNHQR010000000
VERSION     DBNHQR010000419.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5696)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5696)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5696
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1053155"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="AC16VR_09525"
     CDS             complement(<1..586)
                     /locus_tag="AC16VR_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008174102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR6011437.1"
                     /translation="MESMETENPGIETIPLRAYTERAYLDYAMYVINDRALPHIADGL
                     KPVQRRIIFAMSELGLSNQAKFTKSARTVGDVLGKFHPHGDSACYEAMVLMAQPFSFR
                     YPLVDGQGNWGAPDDPKSFAAMRYTEARLSAYAELMLSESKQGTVDFIANFDGTLTEP
                     RLLPARAPFVLLNGSTGIAVGMATDIPPHNMNEVI"
     gene            complement(626..2518)
                     /gene="parE"
                     /locus_tag="AC16VR_09530"
     CDS             complement(626..2518)
                     /gene="parE"
                     /locus_tag="AC16VR_09530"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HIR6011438.1"
                     /translation="MADASKYSAESLQVLSGLEPVRRRPGMYTDTSRPNHLVQEVVDN
                     SVDEALAGYAREIEVTLFKDGSVEVVDDGRGMPVDVHPKHKVSGVELILTRLHAGGKF
                     DNENYSFSGGLHGVGVSVVNALSEWLEVEVKREGKVFRQRYKGGKPTSKLKPEGTVGQ
                     RNTGTRVTFKPDAGYFDSVTVAVGRLKHLLRAKAVLCPGLRVSLNVEGKESESWHYEN
                     GLEGYLVNALAGAEVIPEKPFMHAATGNQEGVDWAVQWVLDGGELITESYVNLIPTAQ
                     GGTHVNGLRSGLIDALKEFCEFRDLLPRGVKLTGDDLWDQCSYVLSAKLKDPQFSGQT
                     KEKLSSRQSAVFIGGVAKDAFSLWLNEHPQDGERLAELAISNAQKRVQAAKKVARKKV
                     TAGPALPGKLADCSGQDAERAELFLVEGDSAGGSAKQARDREFQAVMPLRGKILNTWE
                     VDVSQVLDSQEVHDIALAIGVDPGSPDLSGLRYAKVCILADADSDGLHIATLLCALFL
                     RHFRALVDAGHVFVAMPPLYRIDVGKEVFYALDEAEKEGVLDRIAAEKKRGQIQVQRF
                     KGLGEMNPMQLRETTMNPDTRRLVRLELDSPTKTDEVMDMLLAKKRSPDRRAWLEKKG
                     DQASLA"
     gene            complement(2536..3333)
                     /locus_tag="AC16VR_09535"
     CDS             complement(2536..3333)
                     /locus_tag="AC16VR_09535"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HIR6011439.1"
                     /translation="MKTVLHITDLHLQPRESDRLLGVDTADSLRAVLARALAEGRPDA
                     LLVTGDVSHEGDEASYRRFAAIVADHYEGPVLCLPGNHDKSAAMREVLGETWALDLQE
                     WSLIGLDSHVDGQVGAGLSETDMAALEGRLAASRGRHVLVATHHPPVDVGCPWLDKDR
                     IQNGAELLEWLSEHAGVKGMVFGHAHQVIESAYENLALLGTPATCFQFAPRTERFAVD
                     ERKPGYRWLRLDAQGGLQTEVGRVDDYPLTLDLSERSRSAGRAPKPN"
     gene            complement(3392..3796)
                     /locus_tag="AC16VR_09540"
     CDS             complement(3392..3796)
                     /locus_tag="AC16VR_09540"
                     /inference="COORDINATES: protein motif:HMM:NF018552.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 domain-containing protein"
                     /protein_id="HIR6011440.1"
                     /translation="MAECDANYLRLMKLFPSLREEDVSDFAVCIGDMHYEVCIEVQDR
                     GPYTTLVRLSQLPEAPWSTRPALTVRVYHDARSAEVVEYQRGGHFQAVYDYPNRDMRQ
                     PDEKAQVNRFLGEFLAMCLTHGVSVREPEFAS"
     gene            4078..5466
                     /locus_tag="AC16VR_09545"
     CDS             4078..5466
                     /locus_tag="AC16VR_09545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HIR6011441.1"
                     /translation="MNKPRLIIALLGLAWASAASATDILDAYRDAQSNDPVLGAARAG
                     LDATQELIPQSRSALLPRVAIGGQTSWNERSFPSPVFIEDIGTRVRIPDQEFNEHGWQ
                     AELRQPLFDLEAWFNFRSSQAGVEGAEWGFVSVEQDLIVRTVVAYLNVLRAQDLLDSA
                     KAAEDAVKRQLEQVQQRFDVGLVAITDVLEAQAVYDSAQVVRIQAAGDHDIFFETLRT
                     LTGQPYDSIARISEALPVVDPTPGNEEEWVNTALTGNPTILAANSQLDAAERTLKARR
                     SGHMPTVDATVSTNHFVTGGASFLGGKIDQDVYALSITLPIYQGGLTSSRAREARALA
                     EQARQELLDRQLTVSRDTRNLFRAVATDVVRVRARKKAIESSKSALEATQTGYEVGTR
                     NIVDVLDAQNRLYASEFDYADSRYNYIIDLMQLRQTAGTLNGEDVEQLNVYADSNDPV
                     ERLNSLKARVIN"
     gene            complement(5459..>5696)
                     /locus_tag="AC16VR_09550"
     CDS             complement(5459..>5696)
                     /locus_tag="AC16VR_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011442.1"
                     /translation="FNFPDVVAAFEDSAALLRVGDAAGLAAEVARCLGDAELRERMAA
                     SARRVVAANTGATERLLALLREHVGRVSGGGPLS"
BASE COUNT         1002 a         1900 c         1795 g          999 t
ORIGIN      
        1 tgatgacctc gttcatgttg tgcggcggga tgtcggtggc catgcccacc gcgatgcccg
       61 tgctgccgtt gagcagcacg aagggcgccc gggccggcag aagccggggc tcggtgagcg
      121 tgccgtcgaa gttggcaatg aagtccaccg tgccctgctt ggattccgaa agcatgagct
      181 cggcgtaggc agacaggcgc gcttccgtgt agcgcatggc cgcgaaagac ttggggtcgt
      241 cgggcgcgcc ccagttgccc tggccgtcaa cgagcgggta gcggaaggaa aacggctggg
      301 ccatcagcac catggcttcg taacacgcgc tgtcgccgtg gggatggaat ttgcccagca
      361 cgtcgccgac cgtccgggcc gatttggtga acttggcctg attcgaaagg cccagctcgc
      421 tcatggcaaa gatgatccgg cgctggacgg gcttgagccc gtcggcaatg tggggcaacg
      481 cgcgatcgtt gatgacatac atggcgtagt cgagatacgc ccgctccgta taggcccgca
      541 ggggtatggt ttctatgccg ggattttccg tttccatgct ttccatggcg ccatctggcg
      601 cctccaaatt gcggttgttc gaaggctacg cgaggctcgc ctggtcgccc ttcttctcca
      661 gccaggcgcg gcgatcgggc gaacgcttct tggccagaag catgtccatg acctcgtcgg
      721 tcttcgtcgg tgagtcgagt tcgagccgga ccaatcgacg ggtgtcggga ttcatcgtcg
      781 tctcgcgcag ctgcatcggg ttcatctcgc caagcccctt gaatcgctgc acctgaatct
      841 gcccgcgttt cttctccgcc gcgatccggt ccagcacgcc ctctttctcc gcctcgtcca
      901 gggcgtagaa gacttccttg cccacgtcga tccggtaaag cggcggcatg gcgacgaaca
      961 cgtgtccggc gtcgaccagc gcgcggaagt ggcgcagaaa cagcgcgcag agaagcgtag
     1021 caatgtgcag gccgtccgag tccgcgtccg cgaggatgca gaccttcgcg tagcgcaggc
     1081 cgctcaggtc cggcgagccc ggatccaccc cgatggccag ggcgatgtcg tgcacctcct
     1141 gggagtcgag gacctgactg acatccactt cccaggtatt gagtatcttg ccgcgcagcg
     1201 gcatgaccgc ctggaactct cgatcccgcg cctgcttggc ggagccgccg gccgaatctc
     1261 cttcgacaag aaagagctcg gcccgctcgg cgtcctgacc ggagcagtcc gccagcttcc
     1321 cgggcagagc gggacccgca gtgaccttct tgcgcgcgac cttctttgcc gcctggaccc
     1381 gcttctgcgc gttggagatg gccagctccg ccagccgctc gccgtcctgc gggtgctcgt
     1441 tgagccagag actgaaggcg tccttggcca cgccgccaat gaaaacggcc gactggcggg
     1501 aagagagctt ttccttggtc tgcccggaaa actgcgggtc tttgagcttg gcggagagca
     1561 cgtagctgca ctgatcccag agatcgtcgc cggtgagttt gaccccccga ggaagaagat
     1621 cccggaactc gcagaattcc ttgagcgcgt cgatgaggcc cgaacgcaga ccgttgacgt
     1681 gcgtccctcc ctgagccgtg gggatcaggt tgacgtagga ctcggtaatc agctcgccgc
     1741 catcgagcac ccactgcacg gcccagtcca caccttcctg gttgccagtg gcggcgtgca
     1801 tgaacggctt ctcgggaatt acctcggcac cggcgagcgc gttaaccagg taaccttcca
     1861 gaccgttctc gtagtgccag gactcggatt ccttgccttc cacgtttagg ctcacgcgca
     1921 gacccggaca gagcacggcc ttggcccgca gcagatgctt gagcctgcca acggcgacag
     1981 tgacggagtc gaagtatccg gcatcgggct tgaacgtgac ccgggtcccc gtgttgcgct
     2041 gacccacggt cccctcgggt ttcagcttgc tggtgggctt gccgcccttg tagcgctggc
     2101 gaaagacctt gccttcgcgc tttacttcca cctccagcca ttcggagaga gcgttcacca
     2161 cggaaacgcc cacgccatgc aggccgccgg agaagctgta gttctcgttg tcgaatttgc
     2221 cgccggcgtg aaggcgggtg aggatcagct ccaccccgga aaccttgtgc ttcggatgga
     2281 cgtccacggg catgccgcgg ccatcgtcga ccacctccac ggagccatcc ttgaacagcg
     2341 tgacctcgat ttcccgcgca tatccggcga gcgcctcgtc cacgctgttg tccaccactt
     2401 cctggaccag atggttcggc cgggaggtat cggtgtacat ccccgggcgg cggcgcacgg
     2461 gctcgagccc ggagagcacc tgcagcgatt cagcggaata cttgctggcg tcagccatga
     2521 acggattgga cctcttcagt tgggtttggg ggcccggcca gcgcttcggc tgcgctcgga
     2581 caggtcgagt gtgagcgggt aatcgtcgac ccgacccacc tccgtctgca ggcctccctg
     2641 tgcatccagg cgcagccagc ggtatcccgg cttgcgctcg tccacggcga acctctccgt
     2701 gcgcggagca aactgaaagc atgtggccgg cgtgcccagc agagcgaggt tttcgtaggc
     2761 agactcgatg acctgatgtg cgtggccgaa caccatgcct tttacaccgg cgtgctcgga
     2821 cagccattct agcagctcgg cgccgttttg gatgcggtct ttatcgagcc agggacatcc
     2881 cacgtccacc ggaggatggt gcgtggcgac gagcacgtgg cgccctcgcg atgccgcaag
     2941 ccggccctcg agcgccgcca tgtccgtctc tgaaagcccc gcgcccacct gtccgtccac
     3001 atgactgtcc agcccgatga gcgaccactc ctgcagatcg agcgcccacg tctcgcccag
     3061 cacctcccgc atcgcagcgc ttttgtcatg gttaccgggc aggcagagca ccgggccctc
     3121 gtagtgatcg gcgacgatcg ccgcgaagcg ccggtagctg gcctcatccc cttcgtggct
     3181 gacgtcgccc gtgaccagca gagcgtccgg gcggccctcc gccagggctc ttgccaggac
     3241 ggcacgaagg ctgtccgcgg tgtccacgcc cagcaaccgg tcgctttccc ggggctgcag
     3301 atgcaggtcg gtaatgtgga gaacggtttt caggtttcga tggccgcgcg gccgacgtcg
     3361 ggctccgggg gggtgcgact tccggagccg ctcagctggc gaactccggt tcgcgcacgg
     3421 aaaccccgtg agtcaggcac atggcgagga actcacccag gaatcgattc acctgcgcct
     3481 tctcgtcggg ctggcgcatg tccctgttcg gataatcgta gaccgcctga aagtggccgc
     3541 cgcgctgata ctccaccacc tccgcgctgc gggcatcgtg atagacacga acggtgagcg
     3601 ccggccgcgt gctccagggc gcttcgggta gctggctgag ccgcacgagc gtggtgtacg
     3661 ggcctcgatc ctgtacctcg atgcaaacct cgtagtgcat gtcgccgatg cagacggcaa
     3721 agtcgctcac gtcttcctcg cgcagggagg ggaagagctt catcagccgc agatagttgg
     3781 catcgcattc agccatgtgt gcgggcaaat caatgctgta acgtttgtcg ggcactgtcc
     3841 acgaaaagcc agccatttca cctgccaata taccgacgcc gcgcggaggc gtcaccgttg
     3901 acagcgctgc gacggctaat ttaaggcaac ggcccacaat tccaggggca gatggcgcag
     3961 ggatgggaac cagttcaacg ccccgcccaa gcgtccgacg atcagctcgg cggtgcttcg
     4021 cagggtgcgt ttgctaatat gcgcgctctc ttcctaacag caaagacgga cattgccttg
     4081 aacaaaccca gattgatcat cgccctgctg ggcctggcgt gggcctctgc cgcgagcgct
     4141 accgatatcc tggacgcgta ccgggacgca cagagcaacg accccgttct gggcgctgcc
     4201 cgtgccggcc tggatgcaac ccaggaactg ataccccaga gccgttcggc gctcctgccc
     4261 agagtcgcca tcggcgggca gacgtcctgg aacgagcgga gcttcccgtc acccgtcttt
     4321 atcgaggaca ttggcacccg ggtacggatt cccgatcagg aattcaacga gcacggctgg
     4381 caggccgagc tgcgccagcc ccttttcgac ctggaagcct ggttcaactt ccgcagctct
     4441 caggccggcg tcgagggcgc cgaatggggc ttcgtgtcgg tggagcagga tctgatcgtg
     4501 cgcaccgtgg tcgcctacct caacgtcctg cgggcccagg atctgctgga ctccgcgaaa
     4561 gcggcagagg acgcggtgaa gcgccagctg gagcaggttc aacagcgctt cgacgtgggc
     4621 ctggtggcca tcacagacgt gctggaagca caggccgtct acgacagcgc ccaggtggtc
     4681 cggatccagg ccgcgggcga tcacgacatc ttcttcgaaa ccctgcgcac cctcaccgga
     4741 cagccttacg acagcatcgc gcgcatctcg gaagcgctgc ccgtggtcga cccgacgccg
     4801 ggcaacgagg aggagtgggt gaacaccgca ttgaccggca acccgaccat cctcgcggcc
     4861 aactcccagc tcgacgccgc ggagcgaacc ctcaaggcgc ggcgctcggg gcacatgccc
     4921 acggtggatg ccacggtgag caccaaccat ttcgtcaccg gcggtgcttc ctttctcggg
     4981 ggcaaaatcg atcaggacgt ctacgcgctg tccatcaccc tgcccatcta ccagggcggc
     5041 ctgacctcgt cgcgtgcgcg ggaggctcgg gctctggccg agcaggcgcg tcaggagctg
     5101 ctggaccggc agctgaccgt gagccgggat acccgcaacc tgttccgcgc ggtcgccacc
     5161 gacgtggtcc gggtccgggc gcgcaagaag gccatcgagt ccagcaagtc ggccctcgaa
     5221 gccacccaga ccggctacga ggtaggcacc cgcaacatcg tcgacgtgct ggatgcgcag
     5281 aaccgactct acgcatccga gttcgactac gccgactccc gctacaacta catcatcgat
     5341 ctcatgcagc tcaggcaaac ggccggcacc ctcaacgggg aggacgtcga gcagctgaac
     5401 gtgtacgcgg actccaatga ccccgtggag cggctcaact cgctgaaagc ccgagtcatc
     5461 aactgagcgg accgcctccg ctaacgcggc cgacgtgctc gcgcagaagc gcgagcaacc
     5521 gctcggtcgc tccggtattc gcggcgacga cccggcgcgc ggacgcggcc atcctctccc
     5581 gcaactcggc atcgcccagg cagcgcgcga cctcagcggc aaggccggcc gcgtcgccga
     5641 cccgcaagag agctgccgag tcctcgaacg cggccaccac gtcagggaaa ttgaac
//
LOCUS       DBNHQR010000420         3672 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1495720, whole genome shotgun sequence.
ACCESSION   DBNHQR010000420 DBNHQR010000000
VERSION     DBNHQR010000420.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1495720"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="AC16VR_09555"
     CDS             complement(<1..230)
                     /locus_tag="AC16VR_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011443.1"
                     /translation="MRKSTRASAPVFIITVYYVVLLGGFMAASSYLPGLRVYLPVDGI
                     SELIERGGASFEPVEIIRTAIVEAPHMARLALG"
     gene            complement(230..2083)
                     /locus_tag="AC16VR_09560"
     CDS             complement(230..2083)
                     /locus_tag="AC16VR_09560"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR6011444.1"
                     /translation="MSTLHKPTCLIALFLSVTLMTPPGWTAAREIGPNHWAGVSRVVA
                     FGDVHGAHDQMVTALESAGVVDAELNWAAGDTHLVSLGDLLDRGAHSRQTMDLLMRLQ
                     RQAKGAGGRVHVVLGNHELMNLTGDMRYVAADEFAAFADDEDPSVREAAREAFVAAAD
                     PASGKDPSVEFDERYPAGYFGHRAGFAAAGSYGDWLLQLPLMVVVNETAFVHGGLPAL
                     IADQTLEEINRQYQRDLGEIMALGGRLADAGWVSAERDLLLQDDAVLARLEAAAEGDD
                     GDLSRAGTRFIELVRGPLFGSRGPNWYRGTAMCHASLEETVLKAALKALGAKRVVVGH
                     TPTSNRRVQQRMDGRAILADTGMLRSYYSGQPAPVVLTGDDIEILYPLSETRADAPDV
                     IDPLARYGLDQPRLEAVMSNAAIAEVSTAGTGQRFTTVTRLDEGKRYEFAFERGQGSD
                     NDRKVGAYRLSRLLELNVVPVTVSRTVEGAKGVLIALPEKRVSETERANQGLSRSKWC
                     SFGNDYQLMYAFDALIRNEQRTTDTMIYDQEDWQLTLIGHEASFGNGTDLPAYLAAVE
                     KILPAAMAARMAAMNEDSLTAALGDQLSKRQIRALLKRRDRMIKDWQLQAL"
     gene            2202..2600
                     /locus_tag="AC16VR_09565"
     CDS             2202..2600
                     /locus_tag="AC16VR_09565"
                     /inference="COORDINATES: protein motif:HMM:NF024865.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6011445.1"
                     /translation="MHPNEALFANESFYLAFAAGDSDAMEQLWSRQHPLVCIHPGWPA
                     LVGRESVIDSWRQILANPDQPEVEFYGARAVELGSGIAVVCYEVLPGTVLVAMNLFIA
                     ENGEARLVHHQSGPCGDPPPRDAVVRRLDA"
     gene            complement(2604..>3672)
                     /locus_tag="AC16VR_09570"
     CDS             complement(2604..>3672)
                     /locus_tag="AC16VR_09570"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6011446.1"
                     /translation="QEPSDLWRTRMDAKYRDRLPGVAMDAKGQKFQKTEGFRPLRLQN
                     IKFEGEDALRNQSGHTPEERLADLAADGVDCEILFPNKGLTMWATPDAAFSQAMCRVF
                     NDWAWEVFGPYNDRLAPMACVASADIPGAVEEIQRCARLGFRGVTLPCKPVWGAPDHE
                     ALNYNLPDFDPLWACVQDAGLPVTFHVSTGRDPRTARGNGGAVINYAVHSLAPTMEPI
                     ANICASGVIDRFPKLRFGTIEAGIGWVPWTLAALDEAYKKHHMWVRPKLKMLPSDYFR
                     QNGFASFQEDQPGLDLAREHRLIDNFLWANDYPHHEGTWPHSAEAIERTMGHLTDSER
                     AKILGLNAASIFGFEVPRGKA"
BASE COUNT          624 a         1189 c         1167 g          692 t
ORIGIN      
        1 cccagggcca gccgggccat atggggtgcc tcgacgatgg cggttctgat gatctcgacg
       61 ggctcgaaag aggcgccgcc ccgctcgatg agttctgaga tgccgtcgac cgggaggtat
      121 acccgcagac cgggcagata gctggatgcc gccatgaagc cgcccaggag gacgacgtag
      181 taaaccgtga tgatgaagac gggcgcagat gctcgtgtcg acttgcgcat tacagagcct
      241 ggagctgcca gtctttgatc attcggtccc tccgcttgag caacgcgcga atctgccgtt
      301 tgctcagctg gtcgcctaag gcagccgtga ggctgtcttc gttcatggct gccatgcgcg
      361 cggccatggc tgccggtagg atcttttcta ccgccgccag gtaggcgggc aggtcggtgc
      421 cgttgccaaa gctcgcctcg tggccgatga gcgtcagctg ccagtcttcc tggtcgtaga
      481 tcatcgtatc ggtagtacgc tgctcgttgc gaatcagcgc gtcgaaagcg tacatcagct
      541 ggtagtcatt gccgaagctg caccacttcg aacggctcag tccctggttt gcccgctcgg
      601 tctccgaaac gcgcttctcc ggcagggcga tgagcacgcc tttggcgccc tcgacggtgc
      661 ggctgaccgt tacgggcact acgttgagct cgagcaggcg ggacaggcgg taagcgccga
      721 ccttgcgatc gttgtctgaa ccctggccgc gttcgaacgc gaactcgtac cgtttgcctt
      781 cgtcgagccg cgtgacggtg gtaaaccgct ggcccgtccc ggcggtgctg acttctgcga
      841 tggctgcatt gctcatgacg gcctccagcc gcggctgatc cagcccgtag cgtgccagcg
      901 ggtcgatgac gtcaggagca tcggcgcggg tttcactcag agggtagaga atctcgatat
      961 cgtcgccggt cagcaccacc ggggcgggtt ggccactgta gtaagagcgg agcatgccgg
     1021 tatcggcaag gatcgcgcga ccgtccattc gctgctgaac gcggcggttc gacgtggggg
     1081 tatgacccac gaccacgcgc tttgctccca gggctttcag cgcagccttc aacaccgtct
     1141 cttcgaggga ggcgtggcac atggcggtgc ctcgatacca gttcggtcct cgggatccga
     1201 agagggggcc tcttacgagc tcaatgaaac gcgtgcccgc tcgggaaaga tcgccgtcgt
     1261 ctccctcggc ggcggcttcc agccgcgcca gcaccgcatc gtcctgcagc agcagatcgc
     1321 gctcggcgct gacccatcct gcatcggcca gccggccgcc caacgccatg atctcgccaa
     1381 ggtccctttg gtactgccgg ttgatctctt cgagcgtctg atcggcgatc agcgcgggga
     1441 gcccgccgtg gacgaatgcg gtctcgttga cgaccaccat cagcggcagc tgcagcagcc
     1501 agtctccgta gctgccggcc gcggcaaatc ccgcccggtg accaaagtat ccggccgggt
     1561 accgctcgtc gaactccaca gatgggtcct tgccggaagc ggggtccgcc gcggcgacaa
     1621 acgcctcgcg cgccgcctcg cgcaccgacg gatcttcatc gtctgcaaac gccgcaaact
     1681 catcggcggc cacgtagcgc atgtcgccgg tcagattcat gagctcgtga ttgcccagga
     1741 cgacgtgcac ccggccgcct gctcccttgg cctgcctctg caatctcatc agcagatcca
     1801 tggtctgccg ggaatgcgcg ccccgatcga gcagatcgcc gaggctcacc agatgcgtgt
     1861 cgccggcggc ccagttgagc tcggcgtcga ccacgccagc ggattccaat gcggtgacca
     1921 tctggtcgtg cgcgccgtgg acgtctccga acgcaacgac gcgagacacg ccggcccagt
     1981 gattgggacc gatttccctg gccgccgtcc agcccggtgg cgtcatgagc gtcacgctca
     2041 gaaaaagagc gatcaggcaa gtgggtttgt gcagcgtgct caaagcagaa gtccgacccg
     2101 agtcgccccc atttcacgaa aagccggctc catttgcaac gacggacgcc gggcgaactc
     2161 gccttgagcc attctcccag agcgtggata ctcgcgccat catgcatccg aacgaagcac
     2221 tgtttgccaa cgagtcgttc tatctcgctt tcgcggccgg cgactcggat gccatggagc
     2281 agctctggtc acggcagcac ccgcttgtct gcattcatcc cgggtggccg gcgcttgtag
     2341 gccgtgagtc ggtcatcgac agctggcgac agatcctcgc taatcccgat cagcccgagg
     2401 tggagttcta cggcgcccgg gcggtggagc tcggcagtgg cattgcggtg gtctgttacg
     2461 aggttctgcc agggacggtg ctggtcgcca tgaacctgtt catcgctgag aacggcgaag
     2521 cgcgcctagt ccaccatcag tcggggccct gcggagaccc gccgccgcgc gacgcggtcg
     2581 ttcgccgcct ggacgcctga ctcctaggcc ttcccccgcg ggacctcgaa gccgaagata
     2641 cttgccgcgt tgagacccaa gatctttgct ctctcggaat ccgtcaggtg gcccatggtt
     2701 cgctcgatgg cctcggccga atgaggccat gtgccctcgt ggtggggata gtcgtttgcc
     2761 cacagaaaat tgtcgatcag ccgatgctcg cgggcgaggt ccagcccggg ctgatcctcc
     2821 tggaagctgg cgaagccgtt ctgccggaag tagtcgctgg gtagcatctt gagcttcggc
     2881 ctcacccaca tgtggtgttt tttgtaggct tcgtccagcg ccgcgagggt ccagggcacc
     2941 cacccgatgc ccgcctctat ggttccgaac cggagcttcg ggaaacgatc gatcacgccg
     3001 gacgcgcaga tgttggcgat gggctccatg gtcggggcca gcgaatgcac ggcgtagttg
     3061 atgacggcgc cgccattgcc gcgagccgtg cgcggatcgc gcccggtgga aacgtggaag
     3121 gtgaccggca gacccgcatc ctgcacgcat gcccacagcg gatcgaaatc cggcaggttg
     3181 tagttgagcg cctcgtgatc cggagcgccc cacaccggtt tgcagggaag ggtcacgccg
     3241 cgaaagccca acctggcgca gcgctgaatc tcctcgactg ctccgggtat gtcggcgctc
     3301 gccacgcagg ccatgggcgc gagccgatcg ttgtaagggc cgaaaacttc ccatgcccaa
     3361 tcgttgaaca cccggcacat ggcctggctg aaggcggcat ccggtgtggc ccacatggtc
     3421 agccctttgt tcggaaacag gatttcgcaa tcgacgccgt ccgctgcaag atcggccaga
     3481 cgctcttcgg gtgtatggcc ggattggttg cgcagagcgt cctcaccttc gaacttgatg
     3541 ttctgcagcc gcagaggccg aaagccctcc gtcttctgga acttttggcc cttagcgtcc
     3601 atggcgacac cgggcaggcg atcgcggtac ttcgcgtcca tgcgcgttcg ccacagatcc
     3661 gatggctcct gc
//
LOCUS       DBNHQR010000421         2994 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_736051, whole genome shotgun sequence.
ACCESSION   DBNHQR010000421 DBNHQR010000000
VERSION     DBNHQR010000421.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_736051"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="AC16VR_09575"
     CDS             <1..500
                     /locus_tag="AC16VR_09575"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HIR6011447.1"
                     /translation="ASAATLVRGWGVTCGMECARAHRDFYPDRKAEYGPVLAAFIDYG
                     RAVSREDYGLLETVRAEFRDGLNGCLESADVIIAPCMPELPPPADALADGPRRPSDRA
                     DLITFTAPFNYSGHPTITLPAKLERGSLPRAFQLIARPLGEPTLVRVGSAFESALGLD
                     AHPIP"
     gene            complement(554..946)
                     /locus_tag="AC16VR_09580"
     CDS             complement(554..946)
                     /locus_tag="AC16VR_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011448.1"
                     /translation="MIELVLRLSLWISLPLNAGAAYILAFPSSWPGQLLGLPAAPDPV
                     YAYLAAYLVALLGLAYGWMALQPSIVRPLLWLGALGKGGAFAVAAALWLAGLARAPLA
                     AAVTVDLLLAAFWIAWLTGTRGKASADG"
     gene            complement(972..1811)
                     /locus_tag="AC16VR_09585"
     CDS             complement(972..1811)
                     /locus_tag="AC16VR_09585"
                     /inference="COORDINATES: protein motif:HMM:NF046782.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HIR6011449.1"
                     /translation="MPINPDAVGAEGRPVRRSWSSKDALLYAVGVGSGTDELAFTTEN
                     TKDVAQRVLPTFAVIIGGGGVPMDAIGSFNPALMVHGEQGIELYDEIPPDGEIDSVGK
                     VTAIWDKGSAAVLEFESTSTNVATGKPLLKTRTSLFCRGEGGWGGDRGPSEKVEFPDR
                     EPDHQVTYATREDQALTYRLSGDRNPLHSDPSFAALGGFEKPILHGLCTWGFTGRALL
                     HTLCDGDPSRFKSMNSRFSKPVIPGDELTISIWVVDEGRALFRTTNQNGDAVIDQGVF
                     TYA"
     gene            1959..2855
                     /locus_tag="AC16VR_09590"
     CDS             1959..2855
                     /locus_tag="AC16VR_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR6011450.1"
                     /translation="MSVPTSRTSAAALLACLLVAAAATEAASYRLPEGGEDVIGASIS
                     LTLAYEDTFAGVAQQFGVGYQELVDANPGVDPWLPGDGARIVMPTAYVLPSAPREGIV
                     INVAEYRLYYYPPGRGSVITFPVGVGRMDFPTPLISTKVVTRIENPSWTPTDAARREH
                     AEMGDPLPHVVPPGPDNPLGHLAIQLSAPGYFIHGTNKPFGVGQGVSHGCVRLYPGHI
                     ETLSELVPNGTPVYIVNEPYKVGWRDGELWIEAHRNLYGENQASELVALIVDATEKRD
                     ASVDWRRVDEVSAALTGIPVRL"
BASE COUNT          538 a          985 c          957 g          514 t
ORIGIN      
        1 cggcgagtgc cgcgacgctc gtgcgagggt ggggagtcac ctgcggtatg gagtgcgcgc
       61 gtgcccaccg ggatttctat cccgaccgca aggctgaata cggacccgtg ctggcggcct
      121 tcatcgatta cgggcgggcg gtttcccggg aggattacgg attgctcgaa accgtccgcg
      181 cggagtttcg cgatgggctc aacgggtgcc tggaaagcgc cgacgtcatc atcgctccct
      241 gcatgcccga gttgccgccc ccagccgacg cattggcgga cggaccacga cggccgtcag
      301 atcgggcgga tctcatcacc tttaccgcgc ctttcaatta ttccggtcac ccgaccatca
      361 ccctgccggc gaagctcgaa cggggcagcc tgccgagagc ctttcagctc atcgcgcgac
      421 ccctgggaga gcctaccctc gttcgtgtcg gcagcgcgtt cgaaagcgct ctcggcctgg
      481 acgcgcatcc cattccctga ccgggccccg acgcccggcg tcgctggcta cgcctgaccc
      541 ggaaatcaag cgatcaaccg tcagcgctcg cctttccccg cgtgcctgtg agccaggcga
      601 tccagaacgc cgcgagcagc aggtcgacgg tcacggcggc cgccagggga gcgcgagcca
      661 gaccggcaag ccaaagggct gccgccaccg cgaaggcgcc ccctttgcca agggcgccca
      721 accacagcag gggcctgacg atgctcggtt gcagggccat ccagccgtag gcgagcccga
      781 gcagggccac cagatatgct gcaaggtagg cgtacacggg atcgggcgct gccggcaggc
      841 ccagcagctg acccggccag gacgagggga aagccaggat gtaggcagca ccggcgttga
      901 gcggcaggct tatccagagc gacagccgca gaacaagctc gatcattgcc gctcgccgca
      961 ggcgcacgct atcaggcgta tgtgaacacg ccctgatcga tgaccgcgtc gccgttctga
     1021 ttggtcgtcc tgaacaaagc cctgccttcg tccaccaccc atatcgagat cgtcaactcg
     1081 tccccgggga tcacgggctt ggaaaaccgg ctgttcatgc tcttgaatcg gctcgggtct
     1141 ccgtcgcaaa gggtgtgcag gagggcgcga ccggtgaatc cccacgtgca cagcccgtgc
     1201 agtatgggct tctcgaagcc gccgagcgcc gcaaaggaag gatccgaatg cagcgggttg
     1261 cgatcgcctg agagccggta ggtgagcgcc tgatcctccc tggtcgcgta ggtgacctga
     1321 tggtccggct cgcggtcggg aaattccact ttctcgctgg gccctcgatc gccgccccag
     1381 cccccttcgc cgcggcagaa gaggctcgtt cgtgtcttca gaagcggctt gcccgtggcg
     1441 acgttcgtgg atgtggattc gaactccagc accgccgcgg agcctttgtc ccagatggcg
     1501 gtcactttac ccacgctatc gatctctccg tccggcggga tctcgtcgta gagctcgatg
     1561 ccctgctcgc catggaccat gagcgcgggg ttgaaggacc cgatggcgtc catcggcaca
     1621 ccgccgccgc cgatgatcac ggcgaaggtg ggcagaaccc tttgcgccac gtcctttgtg
     1681 ttctcggtgg tgaaagcgag ctcatcggtg cctgaaccga cgcccacagc gtagagcaaa
     1741 gcatctttgg acgaccagct gcggcgcacc ggacgtccct cggcgccgac ggcatctggg
     1801 ttgattggca tgcgaagatc ctcccgctta catggatgcc cgctaccata gccgtgggcc
     1861 ccattgcgat caacgcgaat gcagccgcgg cagcggactg gacctgggaa tccagatggg
     1921 gacaatggcg tcctcccggc cgatggtttg agaacgactt gtctgtcccg acatcacgga
     1981 cttctgccgc agccctgctg gcctgcctgc ttgttgcggc ggccgcgacc gaagcggcgt
     2041 cctatcggct gcccgaaggc ggagaggacg tcatcggcgc cagcatcagc ctgacgctcg
     2101 cctacgaaga cacctttgca ggtgtggctc agcaattcgg cgtgggctat caggaactgg
     2161 tggatgccaa ccccggggtg gatccctggc tacccggaga tggtgcccgt atcgtcatgc
     2221 ccaccgccta cgtgctgccc tccgcgccaa gggaagggat cgtcataaac gtcgccgagt
     2281 atcggctcta ctactatccg cctggtcgcg gctcggtcat cacgttcccg gtgggggtag
     2341 ggcggatgga cttccccacg ccgctcatca gcaccaaggt ggtcaccagg atagagaacc
     2401 ctagctggac cccaacagat gcggcccggc gggagcatgc agagatgggt gatccgcttc
     2461 cccacgtggt gccgccaggg cctgacaacc ctctggggca tctagccatt cagctgagcg
     2521 cgcctggata tttcattcac ggcaccaaca aaccgttcgg cgtaggtcag ggtgtcagcc
     2581 acggctgcgt ccgcctgtat ccgggccaca tagagacgct gtcggagctc gttccgaacg
     2641 ggacgcccgt atacatcgtc aatgagccct acaaggtggg atggcgcgat ggcgagctct
     2701 ggatagaggc gcaccgcaac ctatatggcg aaaaccaggc ttccgaactc gttgcgctga
     2761 ttgtcgatgc cacagaaaaa cgcgacgcca gtgtggactg gcgtcgcgtc gacgaggtca
     2821 gtgcagcact gaccgggatt ccggtcaggc tttaacggag cactacttcc gggagcagca
     2881 ggtctccgaa atccgctccg cccgctcgtt ggccgcattg gcggcgtcca gcgcacgctc
     2941 cgcggtggac tgagcagccg cggcgtcgct ggccgctcgg gaagcagcag aagc
//
LOCUS       DBNHQR010000422         1877 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1178666, whole genome shotgun sequence.
ACCESSION   DBNHQR010000422 DBNHQR010000000
VERSION     DBNHQR010000422.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1178666"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /gene="murJ"
                     /locus_tag="AC16VR_09595"
     CDS             <1..615
                     /gene="murJ"
                     /locus_tag="AC16VR_09595"
                     /inference="COORDINATES: protein motif:HMM:NF015013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HIR6011451.1"
                     /translation="VDWGMRMGAILGVPAAAALYVLAVPLVASIFLRGALTPLDARMA
                     SLSLQAFAVGLPALVLVKVLAPAYFARRDTATPFRIGAVAVGVNVALNLALFFWMGHV
                     GLALATSASAWVNALMLLRGLVSGDAYRPGRPFLVSTARALAASAVMVAAFAYWVPEP
                     SAWMAAPDGERVVWLAGVVAGGGSVYLLCLIVFGERPRNLLHRA"
     gene            636..1562
                     /gene="ribF"
                     /locus_tag="AC16VR_09600"
     CDS             636..1562
                     /gene="ribF"
                     /locus_tag="AC16VR_09600"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HIR6011452.1"
                     /translation="MEIIRGLHNVKPRHRGCVLTIGNFDGVHHGHQVLLAHLIAKSEE
                     LGVPSMLMTLEPAPREFFAGETVPARLTRFREKVTLLEPTGLHRILCLPFNERIASIP
                     AEWIVEELLHNQLGIRYIVVGDDFRFGKGAAGDYRMLQAAGERYGFEVSHIGTLTFDH
                     ERVSSTRIRECLSAGDFALAEKLLGHPYFIMGRVVYGRQLGRKLGIPTANIQLQRYRA
                     ALEGVFAVSVRGLEKEYQGVANVGIRPTVDGREPLLEVHIFDFDRDIYGTLLTVEFRH
                     KIRDEIAFDGLDALKAQIQEDQRTARAWFANE"
     gene            1555..>1877
                     /locus_tag="AC16VR_09605"
     CDS             1555..>1877
                     /locus_tag="AC16VR_09605"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HIR6011453.1"
                     /translation="MNEKMGRAGMLKWLGLSAIVVVLDQATKWWIVQEFRLGERMDWL
                     PFLSWVRWHNDGAAFSLLSGAGGRWFFVALAVAFAVFIIWELRRLPVGGRLMGWAYAL
                     ILGGAL"
BASE COUNT          277 a          556 c          668 g          376 t
ORIGIN      
        1 gtggactggg gcatgcgaat gggggcgatt ctcggcgtgc cggcggcagc cgcgctttac
       61 gtgcttgcgg ttccgctggt tgcgagcatt tttctgcgcg gcgcgttgac ccctctcgat
      121 gcccgcatgg cctcgctcag cctgcaggcg tttgccgtcg gcctgccggc gctcgtgctc
      181 gtcaaagtgc tggctccggc gtacttcgcg cgccgggaca ccgccacgcc gttccgaatc
      241 ggcgcggtcg ccgtgggcgt caacgtggcg ctgaatctgg cgctcttctt ctggatgggg
      301 cacgtgggcc tggcgctggc gacgtcggct tccgcgtggg ttaatgcgct gatgctgctg
      361 cgcgggctcg tctccggtga cgcttaccgc ccgggccggc cgttcctggt gagtacagca
      421 agggctctgg ccgcgtcggc cgtcatggtg gccgctttcg cctactgggt tccggagcct
      481 tcggcctgga tggccgcgcc cgatggcgag cgcgtcgtct ggctggcggg cgtggtggcg
      541 ggcggtggca gcgtgtacct gctttgcctc atcgttttcg gcgagcggcc gcggaacctg
      601 ctccatcggg cgtgaggcgc ctataatggt gcgccatgga aatcatcaga ggcctccaca
      661 acgtcaaacc ccgccatcgt ggctgcgtgc tcacgatcgg caacttcgac ggtgtgcacc
      721 acggccacca ggtcctgctc gcccatctca tcgcgaaaag cgaggagctg ggcgtgccga
      781 gcatgctcat gacgctcgag cccgcgccca gagagttttt cgccggggaa acggttcccg
      841 cccggctgac ccggtttcgc gagaaggtca cgctgctgga acccacaggg ctccacagga
      901 tcctatgcct tcccttcaac gagcgcatcg ccagcatccc ggccgaatgg atcgtggagg
      961 agctgctgca caaccagctg ggcatccgct acatcgtggt gggtgacgac ttcaggttcg
     1021 ggaagggcgc cgcgggcgac tatcggatgc ttcaggcagc cggggagcgt tacgggttcg
     1081 aggtcagtca catcggcacg ttgacgttcg atcatgaacg agtcagcagc acgcggatca
     1141 gggagtgtct gtcggcggga gacttcgcgc tggcggaaaa gctgctgggc cacccttact
     1201 tcatcatggg acgggtggta tacgggcggc agctcgggcg caagctgggg attccgaccg
     1261 cgaacattca gctgcagcgc tatcgggccg ccctggaggg cgttttcgcg gtgagcgtgc
     1321 gaggcctgga gaaggagtat cagggcgtcg caaacgttgg cattcgtccg accgtggacg
     1381 ggcgcgagcc gctgctggag gtgcacatat tcgactttga cagggacata tacggaacgc
     1441 tgctcaccgt cgagttccgt cacaagatca gggacgagat tgcctttgac ggccttgatg
     1501 ctctcaaggc gcagattcaa gaggaccagc gtacggcaag ggcgtggttt gccaatgaat
     1561 gaaaagatgg gacgtgccgg gatgctgaag tggctggggc tcagcgcgat cgtggtggtg
     1621 ctggatcagg cgaccaagtg gtggatcgtc caggagtttc gtctgggtga gcgcatggac
     1681 tggcttccgt tcttgagctg ggtgcgatgg cacaacgacg gggcggcttt ctcgttgctc
     1741 agcggcgccg gcggacgctg gttcttcgtt gccctcgccg tcgcgttcgc cgtgttcatc
     1801 atctgggagc tcaggcgcct tccggtcggc gggcggttga tgggctgggc ctacgcgctg
     1861 attctcggcg gcgcgct
//
LOCUS       DBNHQR010000423         3802 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_166876, whole genome shotgun sequence.
ACCESSION   DBNHQR010000423 DBNHQR010000000
VERSION     DBNHQR010000423.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_166876"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="AC16VR_09610"
     CDS             <1..377
                     /locus_tag="AC16VR_09610"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR6011454.1"
                     /translation="ITSSLTLLALLVAAIGVHNSLVALRLNQQQGVHLLVMLGVTAGE
                     LARVAVRRALGIGSVAVALALPLGVLMAWLLCAVINPRAFGWTVDLHLALSDVAVPLA
                     LGIGAAVLAGLLPSPRDSAGEI"
     gene            384..1508
                     /locus_tag="AC16VR_09615"
     CDS             384..1508
                     /locus_tag="AC16VR_09615"
                     /inference="COORDINATES: protein motif:HMM:NF018805.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HIR6011455.1"
                     /translation="MRIVTLPALLLTLLIVAPGCQPDEPAGGAMGEGTLRLGAVLGET
                     DAQGFARAEAVVPFAFPGDHGPHPRFRSEWWYLTANVQNEFGAEFGVQFTVFRQALAP
                     GPGPANPWLSNQVFLAHAAVTGVLEQGHWHEERVARGHPGVAGATAEPFRVWVDGWEL
                     AGRNGGFDEQRLSVKADAFAVDLQLDALKRPVLQGDRGLSAKGPEQASYYYSVPRLSA
                     RGTLRIGEVEHQVSGRAWIDREWSTSALSSGQVGWDWFALHLDDSWEIMVYQLRRADG
                     SRDRYDHAVLIDPYGAKHPLGADDFELEPEIFWQDDDGVAWPLQWRLKALDRELRIRA
                     AVPDQRMETSVSYWEGLVGVFDMAGRRTGRGYMELTGYEG"
     gene            1505..2092
                     /locus_tag="AC16VR_09620"
     CDS             1505..2092
                     /locus_tag="AC16VR_09620"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6011456.1"
                     /translation="MSVDSELELRWERLGSEYGNDLILFKTRFDRMRNPRNDAVLRRL
                     VLESVDWVNVVALTADGLSVMVRQFRFGVDYTTLETPGGMVDPGEDSLAAARRELLEE
                     TGYGGGDWTYLGAVEPNPAFHDHLCHHWLASGVSLLQEPAPTPGEAIEVTLMSERAVA
                     DAVQQGVIKHALALSALSRVFELWPRPFRHERDPS"
     gene            2169..>3802
                     /locus_tag="AC16VR_09625"
     CDS             2169..>3802
                     /locus_tag="AC16VR_09625"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR6011457.1"
                     /translation="MSESSTADASSPRESFSRLLNRKSAGSGLVGASILLVDDKPELL
                     NSLYQLMVLHGYEPDKALGGQQALDLLAEKSYDVVLLDLIMPGVSGHDVLDYAARNEL
                     TCKIIVVSGDSSFSGVKHALHCGAFDFVKKPYEAGELIATMETALRQCQLEAQNELME
                     EKVKESEELHRYIVNSSPDLVYMLDRNGCFTFLNDRVESLLGFSRDELIGKHYTELVD
                     DDHLELARNVFNERRTGDRAVSNAEMRLKSRHARRGARLFHAHAIWTELTAMGVYSDP
                     KERTRENFVGTYGIARDISERKEAQEVISFQAYHDLLTHLPNRALLKDRLSLAITQAR
                     RSKRRLAVMFLDLDRFKIVNDTLGHSMGDRLLKAVANRLQSCLRRGDTLSRFGGDEFT
                     LLLPEVRTRDDVVVIATKILDRLGGPFVIDGLELFVGASIGVAMYPEAGETEEALIQN
                     ADIAMYHVKGRGKNGYQFFSEEMNHKYSTRLSMERELRNGLTQEELVVYYQPQVALKT
                     GEIVGVEALVRWRHPERGLVEPGDFLPMAEETGLIT"
BASE COUNT          639 a         1181 c         1308 g          674 t
ORIGIN      
        1 ccattacgtc ctccctgacc ctgctcgccc tgctggtggc cgccatcggc gtgcacaact
       61 ccctcgtcgc cctgcgcctc aatcagcagc agggcgtgca cctgctcgtc atgctgggcg
      121 tcaccgcggg cgagctcgcc cgggtggccg tgcgccgggc gctgggcatc ggcagcgtcg
      181 cggtagcgtt ggccctgccc ctgggggtgc tcatggcatg gctgttgtgc gcggtgatca
      241 acccgcgggc cttcggctgg acggtggatc tgcatctggc attgagcgac gtggccgtgc
      301 cactcgcgct cgggatcggt gctgccgtgc tggcgggact gttgccgtcg ccgcgggatt
      361 cggccgggga gatctgatag ggaatgcgca tcgtaaccct gcccgcgttg ctgctgacgc
      421 tgctgatcgt ggcccccggt tgccaaccgg acgagccggc gggcggcgcc atgggggaag
      481 ggactctgcg gctcggcgcc gtgctgggcg agacggacgc gcagggtttt gcccgggcgg
      541 aggctgtcgt gccgttcgcg ttccctgggg accacggccc tcacccgagg ttcaggagtg
      601 agtggtggta tctgaccgcc aacgtccaga acgagttcgg cgcggagttc ggggtgcagt
      661 tcaccgtgtt tcgccaggcg ctggcgcccg ggcccgggcc agccaacccg tggctgtcca
      721 accaggtctt tctggcgcac gcagcggtta ccggcgtcct ggagcagggg cactggcatg
      781 aggagcgcgt ggcgcgtggc cacccgggcg tcgccggtgc caccgcagag ccatttcgcg
      841 tctgggtgga cggctgggag cttgccggcc ggaacggcgg cttcgacgag cagaggttga
      901 gcgtgaaggc tgacgcgttc gccgtcgacc tgcagctgga tgcgctgaaa aggcccgttc
      961 tgcagggtga tcgcggcctg agcgccaagg ggccggagca ggcttcctat tactactcgg
     1021 tgccgcggct aagtgcgcgg ggcacgctca ggatcggtga ggtagagcat caggtcagcg
     1081 gccgggcctg gatcgaccgc gagtggagca ccagcgcgct ctcgtccggg caggtgggtt
     1141 gggactggtt cgcccttcac ctcgacgaca gctgggagat catggtctac cagctcaggc
     1201 gtgcggatgg gtcccgggat cgctacgatc acgccgtgct catcgacccc tacggcgcga
     1261 agcatcctct tggcgccgac gacttcgagc tcgagccgga gatcttctgg caggacgacg
     1321 acggcgttgc ctggccgctg cagtggcgcc tgaaggcgct cgatcgcgag ctgcgcatcc
     1381 gcgccgccgt tcccgatcag cgcatggaga ccagcgtttc ctactgggaa gggctggtgg
     1441 gcgtcttcga catggcgggc agacgcactg gccgcggcta catggaattg acggggtacg
     1501 aaggatgagt gtggattcgg agctggaact gcgctgggag cgtctgggct cggagtacgg
     1561 caatgacctg attctgttca agacccgctt cgacaggatg cgcaatccgc gcaacgatgc
     1621 ggtgctccgg cggctggttc tggagtccgt ggactgggtg aacgtcgtag cgcttacggc
     1681 cgatgggctc tccgtcatgg ttcgccagtt ccgcttcggc gtggattaca cgactttgga
     1741 gactccggga ggcatggtgg acccgggcga ggactcgctc gccgccgccc ggcgggagct
     1801 cctggaggag accggttacg gcggtgggga ttggacctac ctcggcgccg ttgagccgaa
     1861 tcccgctttt cacgaccacc tctgccatca ctggctggcc tccggcgttt cgttgctgca
     1921 ggagccggcg ccgaccccgg gcgaagccat cgaggtgacg ctgatgagcg agcgggcggt
     1981 cgccgacgcg gtgcagcaag gggtgatcaa gcacgcgctg gcgctttccg cgctgtcgcg
     2041 cgtgttcgag ctctggccgc gcccgtttcg gcacgagcgt gatccgtcat gagcgggcgc
     2101 ctatccagag cagcaaaaat tcgtcacaat tggtaagcat gtcgagcacg acgtgggaca
     2161 gttctggtat gagtgaaagc agcacggcgg acgcttccag tccccgagag tcattctccc
     2221 gattgctcaa tcgaaaaagc gccgggtccg ggctggtggg tgcgtcgatt ctcctcgtcg
     2281 atgacaaacc cgagctgctc aacagccttt accagctgat ggtgctgcac ggctacgagc
     2341 cggacaaagc cctcggcggc cagcaggcgc tggatcttct ggcggagaaa agctacgacg
     2401 tcgttctgct ggacctgatc atgccgggcg tgagcgggca cgacgtcctc gactacgcgg
     2461 cccgcaacga gctcacctgc aaaatcatcg tcgtcagcgg cgattccagc ttcagcggcg
     2521 tcaagcacgc gctgcattgc ggggccttcg acttcgtcaa gaagccctat gaggcgggcg
     2581 agctcatcgc gaccatggag acggcgctgc gacagtgcca gctcgaggct caaaacgagc
     2641 tcatggaaga gaaggtcaag gaatcagagg agctgcatcg ctacatcgtc aacagctccc
     2701 cggatctggt ctacatgctc gaccgcaacg gctgcttcac ctttctcaac gaccgtgtcg
     2761 aaagtctgct gggattctcc cgtgacgaac tcatcggcaa gcactacacg gagctggtgg
     2821 acgacgacca cctggagctt gcgcggaacg tgttcaacga gcggcgcacg ggcgacaggg
     2881 cggtgtccaa cgcggagatg cgcctgaaga gccgccacgc ccgacggggc gcgcgtctgt
     2941 ttcatgccca cgccatctgg actgagctca cggccatggg cgtttattcc gatcccaagg
     3001 agcgtacccg ggaaaacttc gttggaacct acggcatcgc gcgggatatc agcgaacgca
     3061 aagaggccca ggaggtcatc agctttcagg cctaccacga tctgctcacc catctgccga
     3121 accgcgctct gctcaaggac cgcctgtcgc tggcaatcac ccaggcacgg cggagcaagc
     3181 gaagactggc cgtgatgttc ctggatctgg accgcttcaa gatcgtcaat gacaccctgg
     3241 gccacagcat gggcgatcgg cttctcaaag cggtagccaa tcgcctgcag agctgtctgc
     3301 ggcgcggcga cacgctgtcg cgcttcggcg gcgacgagtt cacgctgctg ctgccggagg
     3361 tgcgcacgcg cgacgacgtt gtggtcatcg ccaccaagat cctcgaccgg ctgggcggtc
     3421 ccttcgtcat tgatggcctc gagctgttcg tgggtgccag catcggcgta gccatgtacc
     3481 cggaggccgg ggagaccgag gaagcgttga tccagaacgc cgacattgcc atgtaccacg
     3541 tcaagggtcg cggcaagaac ggctaccagt tcttctccga ggagatgaac cacaagtact
     3601 cgacccggct gtccatggag cgcgagcttc gcaacgggct tacccaggaa gagctggtcg
     3661 tgtactacca gcctcaggtg gcgctgaaga ccggggagat cgtcggtgtc gaggcgctcg
     3721 tccggtggcg gcatccggag cgcgggctgg tagagcccgg cgacttcctg cccatggccg
     3781 aagaaaccgg tctgatcacc ca
//
LOCUS       DBNHQR010000424         2219 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1179243, whole genome shotgun sequence.
ACCESSION   DBNHQR010000424 DBNHQR010000000
VERSION     DBNHQR010000424.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1179243"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /gene="lpxD"
                     /locus_tag="AC16VR_09630"
     CDS             <1..1031
                     /gene="lpxD"
                     /locus_tag="AC16VR_09630"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HIR6011458.1"
                     /translation="YTLGELADHIQARLVGDANAVINGLGSLATAEAGEVSHLSSPAY
                     RQHLAGTRATALILREADLPLWDGNALVSENPYLSFALVSQLFTRRPELAEGLHPTAE
                     IHPEAVVEPGARLGPGVVVGARTRVSAGARLHAHVVVGEDCRIGEEVTLMPGVVIYPR
                     VKIGARSTAHSGVVIGADGFGFTPSSNGSLEPIAQLGGVSIGADVSIGAGTTIDCGAI
                     EDTVIEDGVKIDNQVQIGHNCHIGAHTVICGCVGIAGSTRIGRHCVLAGKCGIGGGGP
                     VELADGVVLSGGTNVMVSIDRPGVYSGSVLHETHPRWKRNAMRLRRLDDLFRRVADLE
                     KKVKDQTG"
     gene            1092..1529
                     /gene="fabZ"
                     /locus_tag="AC16VR_09635"
     CDS             1092..1529
                     /gene="fabZ"
                     /locus_tag="AC16VR_09635"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HIR6011459.1"
                     /translation="MKDITEIFDYLPHRYPLLLVDRVLEIVPGERIRGFKNVTFNEDF
                     FNGHFPGNPVMPGVLIVEAMAQLSGVLAFETHDRRPSDGYLYYLASIDNTRFKRPVVP
                     GDRLMMESELVADRRGLMKFKLAAFVDDDLACTAEIACMERKL"
     gene            1531..>2219
                     /gene="lpxA"
                     /locus_tag="AC16VR_09640"
     CDS             1531..>2219
                     /gene="lpxA"
                     /locus_tag="AC16VR_09640"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HIR6011460.1"
                     /translation="MIDPRAIIDPSAVIGEGVTIGPWTFIGPDVVIGDGCWVAPHVII
                     RGPTRIGRNNKIYQFATVGEDTPAMAYHGEPTTLEIGDDNVIREGVTIHRGMMQDLGR
                     TVIGSHCLLMAYVHVGHDCIVGDHVIMANNSSISGHVRVGDHANFGGYSGVPQFRRIG
                     AYSHISGMSLVLKDVPAYMTVMGNPASAIGLNIEGMRRRGYSKELIDALRSAHRVVYR
                     EGRTSQDACRRL"
BASE COUNT          412 a          675 c          743 g          389 t
ORIGIN      
        1 cctatacgct tggagagctc gctgaccaca tccaggcccg cctcgttggc gacgccaatg
       61 cggtaatcaa cgggctgggg agcctcgcga cggccgaggc gggcgaagtg agccatcttt
      121 cgagccccgc gtaccgacaa catctggccg gtacgcgggc aaccgcgctg attctccggg
      181 aggcagatct gccgttgtgg gacggcaacg ccctggtctc ggagaaccca tacctgtcat
      241 ttgccctggt ttcacagctc ttcacccggc gacccgagct tgcggaggga cttcacccca
      301 cagcggagat tcatcccgag gcggtggtcg agcccggtgc ccggctgggc ccgggcgtgg
      361 tcgtgggggc gagaacccgg gtgtccgcgg gtgccaggct gcacgcccac gtcgtggtgg
      421 gggaagactg ccggatcggc gaggaggtaa ccttgatgcc gggggtagtg atctaccctc
      481 gcgtgaagat cggcgcgcgc agcacggccc acagcggcgt ggtgatcggg gccgacggtt
      541 tcgggttcac gccgtcatca aacggtagcc tggaacccat tgcccagctc ggcggcgtgt
      601 caatcggcgc ggacgtcagc atcggcgcgg gaaccaccat cgactgcggc gccatcgaag
      661 acacggtcat cgaggatggg gtcaagatcg acaaccaggt gcagatcggc cacaactgcc
      721 acatcggcgc ccacaccgtg atctgcgggt gcgtggggat tgcgggaagc acacgaatcg
      781 gcagacactg tgtgctcgcc ggcaaatgcg gcatcggcgg aggcgggcct gtggagctgg
      841 ccgatggggt agtgctcagt ggcgggacca acgtgatggt ttccatcgat cggcccggtg
      901 tgtattcggg ctcggtgctg cacgagaccc atccgcgctg gaagcggaac gccatgcggc
      961 tgcgccggct ggacgatctg ttccggcgag tggcggacct cgagaagaag gtaaaggacc
     1021 agacaggctg atcggcagtt ttggctttcg ggtcgatcag cgcaaacccg acaaagaacg
     1081 tggtcatcgg catgaaagac atcaccgaaa tcttcgacta tctcccccat cgctacccgc
     1141 tgcttctcgt ggaccgcgtg ctggaaatcg tcccggggga gcggattcgg ggattcaaga
     1201 acgtgacctt caacgaagac ttcttcaacg gtcactttcc aggcaacccc gtgatgccgg
     1261 gcgttctcat cgtcgaagcc atggcgcagc tctcgggcgt gctggccttc gagacccatg
     1321 accggcgccc ttcggatggc tacctctact atctggcgag catcgacaac acccgattca
     1381 agcgtccggt ggtgcccggt gaccgcctga tgatggagag cgagctggtg gcagaccggc
     1441 gggggctgat gaaattcaag ctcgcggcgt tcgtggacga cgatcttgcc tgcactgcgg
     1501 agatcgcctg catggaacgg aagctctgag gtgatcgacc cgcgcgccat catcgacccg
     1561 agcgcggtca tcggggaagg cgtcactatc ggtccgtgga cgttcatcgg ccccgacgtg
     1621 gtgatcggcg acggctgctg ggtcgcgccc catgtgatca tcaggggccc aacccgaatc
     1681 ggccgcaaca acaagattta ccagttcgcc acggtgggcg aggacacgcc ggccatggcg
     1741 tatcacgggg agcccaccac cctggagatc ggcgacgaca acgtgatccg cgagggcgtc
     1801 accattcatc gcggcatgat gcaggatctc ggcagaaccg tgatcggcag ccattgtctg
     1861 ctcatggcat acgtgcacgt gggccatgac tgcatcgtgg gcgatcacgt gatcatggcg
     1921 aacaactcgt ccatctccgg gcatgtgagg gtaggagatc atgccaattt cggcggttac
     1981 tcgggcgtcc cgcagtttcg ccggatcggc gcctactccc acatttcagg catgagcctg
     2041 gtgctgaagg acgtgccggc ctacatgacg gtgatgggca atccggcctc tgccattggc
     2101 ctgaacatcg aagggatgcg gcggcgcggt tactccaagg agctcatcga cgcgcttcgc
     2161 agcgcgcaca gggtcgtcta tcgcgagggc aggaccagtc aggatgcctg tcgacgcct
//
LOCUS       DBNHQR010000425         1958 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_103469, whole genome shotgun sequence.
ACCESSION   DBNHQR010000425 DBNHQR010000000
VERSION     DBNHQR010000425.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_103469"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /locus_tag="AC16VR_09645"
     CDS             <1..947
                     /locus_tag="AC16VR_09645"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR6011461.1"
                     /translation="IGGAAFITRPKVGISVYPTDGDDSTSLIRCAQRALLSSSDLRGG
                     RVHFHCEAMNDVIIDRLINEAALRRAFENGGFHLVYQPKVELSTGRTTGVEALLRLVD
                     PELGELPPEEIVPLAEQVGLISELTRWVLAEVCGQIRRWRQAGLPRVPVSVNVSATDM
                     LTPDFADFLTGLLLDNGVAPQALELEIPCRSFDGANDGMAAMVAELRTVGVTIAVGDF
                     GADGSALDCLDHLPVDALKIDRHLIREITRSRISTAITAGAIAASRSLNLKVVAAGVE
                     TVEQVRLLRELDCLEVQGAAYSMPVSPEQLADWLIRTR"
     gene            complement(948..>1958)
                     /locus_tag="AC16VR_09650"
     CDS             complement(948..>1958)
                     /locus_tag="AC16VR_09650"
                     /inference="COORDINATES: protein motif:HMM:NF012363.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HIR6011462.1"
                     /translation="GLLDQIRALEWVRDNIQQFGGDPDRVTIAGESAGAFSVCSLLGS
                     PLAQGLFHRAIAQSGAAHHTLRKATAERITDLFLESLGIDDPTDLDSLPVERILDAQT
                     EIVQRLEDRSGALAVLGELHDPVGPFYPCVGNAVLPQSPIEAIRSGSGASIPLLIGTN
                     ADEATLWGYGDVPEQRLHEVVQAYNASAPLETLRAQRPKAATDQLMIALTTDFLFRIP
                     AIRVAEARAGFDTATWMYLFSWKSRAFGGRLGATHALEIPFVFDNLDRAGVDIFLGEG
                     PTPQALADVMHRAWIRFVQGDDPGWERYEPGSRATMRFDDPCELIYDPDREEREAWDG
                     YR"
BASE COUNT          338 a          600 c          668 g          352 t
ORIGIN      
        1 agatcggcgg cgcggcgttc atcacgcgac cgaaggtcgg gatcagcgtt tacccgacgg
       61 acggcgacga ctccacatcg ctcatccgct gtgcccagcg cgcgttgctg tcatcgagcg
      121 atttgcgcgg tggccgagtc catttccact gcgaagccat gaacgacgtg atcatcgacc
      181 ggctgatcaa cgaggcggcg ctgcgtcgag cattcgaaaa cggcggattc cacctggttt
      241 accagccgaa ggtggaactc tcgaccggcc gcaccaccgg cgtggaagct ctgctgcgcc
      301 tggtcgaccc ggagctgggc gagctgcctc cggaggagat cgtcccgctt gcggagcagg
      361 tgggcctgat cagcgagctc acccgatggg tgcttgccga ggtgtgcggg cagattcgac
      421 gctggcgaca ggccgggctc ccacgagtac ccgtgtccgt caacgtgtcc gccacggata
      481 tgctgacgcc cgatttcgcc gacttcctga ccggcctgct gctcgacaac ggcgtcgcgc
      541 cccaggctct ggagttggag ataccctgcc ggagtttcga cggggcgaat gacgggatgg
      601 cggctatggt cgccgagctg agaactgtcg gtgtgaccat cgccgtgggc gactttggcg
      661 ccgacggatc ggcgttggat tgcctggatc accttccggt ggacgctttg aagatagacc
      721 gacacctgat tcgcgagatc accagaagca ggatcagcac ggcgatcacc gctggtgcca
      781 tcgcggcttc gcggtcattg aatctcaagg tggtggcggc gggcgtggaa accgtggagc
      841 aggtgcgcct tctgcgcgag ctcgactgcc tggaggttca gggagccgca tactccatgc
      901 cagtttcgcc cgagcagctg gctgactggc tgattcgcac ccgctgatca gcgatagccg
      961 tcccaggctt cgcgttcctc acgatcgggg tcgtagatca gctcgcaggg atcgtcgaag
     1021 cgcatggttg cccgggagcc cggctcgtat cgctcccaac cgggatcgtc cccctgcacg
     1081 aagcgtatcc aggctctatg catgacgtct gcgagcgcct ggggggttgg tccttcgccc
     1141 aggaatatgt ccacgcccgc ccgatccagg ttgtcgaaaa cgaaggggat ttccagggca
     1201 tgagtcgctc cgaggcggcc gccgaacgcc cgtgatttcc aggagaagag atacatccac
     1261 gtcgcggtgt cgaagccggc tcgcgcctcg gccacccgga tggcggggat ccggaaaaga
     1321 aagtcggtgg tcagagcgat catcagctga tccgtcgccg ctttgggccg ctgggcgcgg
     1381 agagtttcca gcggcgccga ggcgttgtag gcctggacga cctcgtgaag cctttgctcg
     1441 ggaacgtctc cgtaacccca cagcgtagcc tcgtcggcat tggttccaat gagcagcgga
     1501 atgctggcgc cggagccgct gcggatggcc tcgatggggc tctgtggcag aacggcgtta
     1561 cccacgcaag ggtagaaggg tcccacggga tcgtggagtt cccccagaac ggcgagagcg
     1621 ccggagcgat cttcgagccg ctgcacgatc tcggtctggg catcgaggat gcgctccacc
     1681 ggcagcgagt ccagatctgt cgggtcgtcg atgcccagcg attcgagaaa gaggtcggtg
     1741 atccgctcgg cagttgcctt gcgcagggtg tggtgggcgg ccccgctttg cgcaatcgcc
     1801 cggtgaaaca gtccctgggc gagaggtgag ccgagcagcg agcagacgct gaaagcgccc
     1861 gcagactcac cggctatcgt gacccggtca gggtcgccgc cgaattgctg gatgttgtcc
     1921 cgtacccatt cgagagctcg gatctggtcg aggaggcc
//
LOCUS       DBNHQR010000426         2950 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_41117, whole genome shotgun sequence.
ACCESSION   DBNHQR010000426 DBNHQR010000000
VERSION     DBNHQR010000426.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_41117"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1464
                     /gene="mnmG"
                     /locus_tag="AC16VR_09655"
     CDS             <1..1464
                     /gene="mnmG"
                     /locus_tag="AC16VR_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HIR6011463.1"
                     /translation="LGGRIHIGLDNHPGGRAGDAPSNALAERLRALPFRVDRLKTGTP
                     PRIDARSVDFSVMAEQPGDEPIPCLSFLGSPDDHPRQVSCHVTHTNDRTHSLIRAALD
                     RSPLYTGVIEGVGPRYCPSIEDKVVRFSERTEHQIFVEPEGLDTHELYPNGISTSLPF
                     DVQVALVRSIAGFERAHITRPGYAIEYDFFDPRDLHYSLETKALEGLFFAGQINGTTG
                     YEEAAAQGLLAGTNAALKVAGRAPWCPRRDEAYLGVLVDDLVNMGTKEPYRMFTSRAE
                     FRLRLREDNADHRLTPVGRDLGLVGEKRWDAYTTKRERVERVHGRMATRNVIAGSEVA
                     DRLASLTGERMTRDQTLITYLKRPGVGIAALVGLLGDVLDLQDGDLDGQVLAQVEVEA
                     KYEGYIARQEEEIARIRRHEGLKLDEGLDFAGIEGLSAELKQKLTEARPSTLARAARI
                     PGMTAAGLSVLLVHARRARATGAVAAPEDEPARGSGG"
     gene            1457..2092
                     /gene="rsmG"
                     /locus_tag="AC16VR_09660"
     CDS             1457..2092
                     /gene="rsmG"
                     /locus_tag="AC16VR_09660"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HIR6011464.1"
                     /translation="MAEAIAEELRGGAAELGMTLSACQVEKLVAYADLLRRWNDKFNL
                     ISRRDVPRLVSRHLLDSLSISPWLGGETTLDLGSGAGLPGIPLAIAHPEREFLLADRS
                     ARKARFVEQAAMRLGLSNVSVFAGDAAALEQSFACVVARAVAGPAELWRIAGHLVRPG
                     GRLVCMSRTAGSGEGATLAADVPDAGSVLAERVAVPGNAGPHEVIVIEAAA"
     gene            2089..2865
                     /locus_tag="AC16VR_09665"
     CDS             2089..2865
                     /locus_tag="AC16VR_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015278981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR6011465.1"
                     /translation="MTRIIAVANQKGGVGKTTTSVNLSASLALSGSRVLLIDLDPQGN
                     ATMGSGVEKGGLERSIYHWLMEEVPLDDVLQRCASGYALLPSNTDLTAAEVELLSQPQ
                     REHRMRRRLAEDAARFDFVIIDCPPSLNILTLNALIAADGVLIPMQCEYYALEGLTAL
                     LETVEGVRQQANPSLSIEGLLRTMYDPRNALTRDVSRQLIQYFGDRVFRTVIPRNVRL
                     AEAPSHGLPAILYDRQSRGAVAYMALAGEIKRKHEEDDDP"
BASE COUNT          496 a          935 c         1007 g          512 t
ORIGIN      
        1 ctcggcggcc gtattcacat cggcctggac aatcaccccg gcggccgagc gggagatgca
       61 ccgtccaacg cgctggcgga gcgtctgcgc gcgctgccgt tccgcgtgga ccggctgaag
      121 acgggtaccc ccccgcgaat cgacgcccgc tccgtggatt tctcggtgat ggccgagcag
      181 cctggggatg agccgatccc ctgcctgtct tttctcggct cccccgacga tcacccccgt
      241 caggtcagtt gccacgtcac ccataccaac gatcggaccc actcccttat tcgcgcggcg
      301 ctggatcgct cacctctcta caccggggtc atcgaaggcg ttgggccgcg atactgtccg
      361 agtatcgagg acaaggtcgt gcggttctcg gagcgaactg aacatcagat attcgtcgag
      421 ccggaagggc tggacactca cgagctctac cccaacggga tctccacgag cctgccgttc
      481 gatgtgcagg tcgccctggt gcgcagcatc gccggattcg agcgggccca catcacgcgg
      541 ccgggctatg ccatcgagta cgactttttc gacccccggg acctgcatta ctcgctggag
      601 acgaaggcgc tggaagggct gtttttcgcc ggtcagatca acggcaccac cggatacgag
      661 gaggcggcgg ctcaaggcct gctcgccgga acgaacgcgg cgctcaaggt ggccggccgg
      721 gcgccctggt gtccccgccg cgacgaggcc tatctgggcg ttctggtgga tgatctggtg
      781 aacatgggca ccaaggagcc ctatcggatg ttcaccagcc gggcggagtt ccggctgagg
      841 cttcgcgaag acaacgcgga ccatcgcctc accccggtag gccgggatct ggggctcgtg
      901 ggcgaaaagc gctgggatgc gtacaccacg aagcgtgagc gggtcgagcg cgtccacggg
      961 cggatggcga cccgcaacgt aatcgcgggg tcggaggtgg ctgaccggct cgccagcctc
     1021 accggtgagc ggatgactcg agatcaaacg ctcatcacct atctcaagcg tccgggcgtg
     1081 ggcatcgccg cgttggtcgg tctccttggg gatgtcctgg acctgcagga tggggacctc
     1141 gacggccagg tgctggcgca ggtggaggtg gaagctaagt acgagggcta cattgcgcgc
     1201 caggaagaag agattgccag aatccgtcgg cacgagggcc tcaagctcga cgaaggcctg
     1261 gacttcgccg gtatcgaagg gctttccgca gagctcaagc agaagctgac cgaggctcga
     1321 ccctcgacgc ttgctcgcgc tgcgcgtatc cccggaatga cggcggccgg cctgtccgtt
     1381 ctgctggtgc acgcccgaag agcccgggct acaggtgctg tcgccgcccc ggaagacgag
     1441 ccggcgcgtg gttccggtgg ctgaggcgat cgccgaggag ctgcgggggg gcgccgccga
     1501 gctgggaatg accctatcgg cctgccaggt ggaaaagctc gttgcctacg ccgatctcct
     1561 gcgccgatgg aatgacaagt tcaacctgat ctcgcggcgg gatgttcctc ggttggtatc
     1621 ccgccacctc ctcgacagtc tgagcatctc gccatggctg ggcggggaga ctactctgga
     1681 cctcggctcg ggggccggcc tgcctggaat ccctctggcc atcgcccatc cggaacgcga
     1741 attcctgctg gctgaccggt cggcgcgcaa ggcgcggttt gtcgagcagg cggcgatgcg
     1801 tctaggcctt tcgaacgtga gcgtgtttgc cggggacgcc gccgccctgg agcaatcgtt
     1861 cgcttgtgtg gttgcccggg cggtggccgg acctgccgag ctgtggcgta ttgccgggca
     1921 cctggttcgt ccagggggaa ggttggtctg catgagccgg acggcaggct ccggggaagg
     1981 cgcaaccctt gccgccgacg tgcccgacgc cggtagcgtt ctcgccgagc gggtggcagt
     2041 tcctggcaac gcagggcccc acgaggtgat cgtcatcgag gccgctgcgt gacgaggatc
     2101 atcgcggttg cgaatcagaa agggggggtc ggcaagacca ccaccagcgt gaacctgagc
     2161 gcatcccttg cgctttccgg ctcccgggtt cttctcatcg acctggatcc ccagggcaat
     2221 gccaccatgg gctctggtgt ggaaaagggt gggcttgagc gcagcattta ccactggttg
     2281 atggaggagg tcccgctgga cgacgtcctg cagcgatgcg ccagcggcta cgccttgctg
     2341 ccgtccaaca cggatctcac tgccgccgag gtggaactgc tgagccagcc gcagcgcgag
     2401 cacaggatgc ggcggcggtt ggccgaggat gcggcgcgct tcgacttcgt catcatcgac
     2461 tgcccaccat cgctgaacat actgaccctc aacgctctga tcgccgctga cggcgtgctc
     2521 attcccatgc agtgcgagta ctacgccctc gagggcctga cggctctgct cgaaacggtc
     2581 gaaggcgtcc ggcagcaggc caacccctcg ctgtccatag agggcctgtt gcgaaccatg
     2641 tatgatcccc gcaacgccct cacgcgggac gtttcccggc agctgatcca gtatttcggc
     2701 gaccgggtgt tccgaacagt gatcccgcgg aacgtccggc tggcagaggc gcccagtcac
     2761 gggcttccag ccattctcta cgatcggcag tcgcggggcg cggtggccta catggcgctt
     2821 gcgggggaaa tcaagcgcaa gcacgaagag gacgacgacc cgtgagcaat tgcgcgtgac
     2881 caagaagaga ctgggcagag gtctggacgc gctgttgtcg ggcgaggttg tggccgatct
     2941 gggcgcgggt
//
LOCUS       DBNHQR010000427         4353 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_483730, whole genome shotgun sequence.
ACCESSION   DBNHQR010000427 DBNHQR010000000
VERSION     DBNHQR010000427.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4353)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4353)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4353
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_483730"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..1510)
                     /locus_tag="AC16VR_09670"
     CDS             complement(65..1510)
                     /locus_tag="AC16VR_09670"
                     /inference="COORDINATES: protein motif:HMM:NF013696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR6011466.1"
                     /translation="MSDAIAPFVNELWDRSIIPTLSDYVRIPNKSPAFDADWEAHGYM
                     QAAVELLRGWCSVPDIDGLTTEIVTIPGRTPVLFCEVPATAPDAGTVLLYGHYDKQPE
                     FDGWEPGLGPWEPVLRDGKLYGRGGADDGYALFGSLAAIAALQAEGIPHGRCVLLIEG
                     CEESGSFDLPAYVELLEERIGQPDLVICLDAECGNYEQLWTTTSLRGMLPGVLTVKVL
                     SEGQHSGAAGGIVPSSFRILRALYDRVEDADTGSLHDCLYVPIPDDARRQLADVAQVL
                     GATTIDRFPWLDGVTPDGDDLQDLLLANAWEPSLATVGLAGAPDVGNAGNTLRPGTSA
                     KLVFRLPPTLDADEAARTIKAELERSPPNGAHVRFELETPQTGWCAPPVAPWLESALM
                     RASQQYFGKPAMYMGMGGTIPFMKMLGDSFPGVQFVVTGVLGPRSNAHGPNEFLEIAT
                     GKRITACVAAVLAEHARRHARRQARRQARQN"
     gene            complement(1507..3348)
                     /locus_tag="AC16VR_09675"
     CDS             complement(1507..3348)
                     /locus_tag="AC16VR_09675"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR6011467.1"
                     /translation="MVLATVLALLAPSLSFAEPDPELETITVTAHRIRTDRPVLPVLT
                     RTTGTGPAVGADALRSLPSLAISQAGSLGSVTQVRVRGSEANHLMVLMDGVELADPAT
                     DASFNFANLSLTGTTRVELLAGAHSAIWGSDAVAGVLNFITKPTERRRRLSLEGGSFD
                     TRQAALQLADTTGSYHYNLALSEFRTDGTNIATTGEEDDGFDQSSYRLSGGYRSESWT
                     IEALVHHLKSDADFDPIPFPAFQPRDGDRVSKHDEYLGSVAATWHGDERFEQQIRLSY
                     FDTDNRSFADGARESRTAAARFKATSLTRLRASSRHAVTLMLEHEKERFEQRGAPSIF
                     GDPNQNQSVRTNSLALEYLWAPLDDLSLALSARTDDNSDFENKESYRAAARYRLTGAV
                     ELWANWGTGIKNPSFVERFGFTPDTFLGNPDLDPETNRHLSAGAVIGHRQWTLRATAF
                     RDRLEDEINGFYFDGAAGAFTSINEPGTSKRDGVEVETTLAIPTGVLSMGASWLDAAE
                     PDAAREIRRPQWQAYATVSHERGPFSAELSGYYVGNREDLDFSGFPAERVRLGDYVLL
                     RAHLAYRLGRHLKLALRGENLLDESYQDQLGYASPGRAVYLQVAIDL"
     regulatory      complement(3395..3604)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(3607..>4353)
                     /locus_tag="AC16VR_09680"
     CDS             complement(3607..>4353)
                     /locus_tag="AC16VR_09680"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR6011468.1"
                     /translation="VTLTFGSQEICHDVVIGCDGEASAVRAGMGYAETTQTTQNVVWT
                     GTCPGTDLPIGLRANAVTTWLGPRAYLTQWPSADGCAARFLAFARNPGAGAKLAGAFR
                     NWHEDVFRLVDSAIGVTRSAPVEREPIAQWYDRRVALLGDACHPVTPHLQQGASLAME
                     DAWVLSRMLERWEDEPAKGFPDYERYRKVRLGRFRQGQRHHLAELTTDPGGKAWRRNL
                     RLSLTSRFLPEIAMQRMDWLYGYDCIKGFE"
BASE COUNT          665 a         1418 c         1525 g          745 t
ORIGIN      
        1 gactttctgc gaaagagctt cggctcgaac agccggctgc tgcaataggc gtcggggcgt
       61 ccggctagtt ctgccgcgcc tgccgccgcg cctgccgccg cgcatgccgc cgcgcatgtt
      121 cggccagcac ggcggcaacg caggcggtaa tgcgcttgcc cgtggcgatt tctagaaact
      181 cgttcggccc gtgggcgttt gagcgtggcc cgagaacgcc ggtcacgacg aactgcacgc
      241 ccgggaagct gtcgcccagc atcttcatga acgggatggt gccgcccatg cccatgtaca
      301 tggccggctt gccgaagtac tgctgggatg cgcgcatgag cgcgctttcc agccacggcg
      361 cgaccggcgg cgcgcaccat ccagtttgcg gagtctcgag ctcgaagcgg acgtgggcgc
      421 cgttgggcgg gctccgctcc agttccgcct tgatggtccg cgcggcctcg tctgcatcca
      481 gggttggcgg cagccggaag accagcttgg cgctggtgcc cggtctgagg gtgttgcccg
      541 cgttccccac gtccggagcg ccggcaaggc ccacggtggc gaggctaggc tcccatgcat
      601 tggccagcag caggtcctgc aggtcgtcgc cgtcgggagt gacgccgtcc agccagggga
      661 agcggtcgat ggtcgtggca ccgagcacct gggcgacgtc tgcgagctga cggcgcgcat
      721 cgtcggggat gggcacatac aggcaatcgt gcagggaacc ggtgtcggca tcctccaccc
      781 gatcgtagag cgcgcgcagg atccggaagc tcgagggaac gatgccgccg gcggctccgg
      841 agtgctgccc ctcgctgagc accttcacgg tcagcacgcc ggggagcatg ccgcgcagcg
      901 aggtggtggt ccacagctgc tcgtagttgc cgcactcggc gtcgaggcag atgacgagat
      961 cgggctggcc gattcgctcc tcgagcagtt ccacgtaggc gggcagatcg aagctgccgc
     1021 tttcctcgca gccttcgatg agcagcacgc atcggccgtg gggaatgcct tccgcctgca
     1081 gggcggcaat ggcggccagc gagccgaaca gggcgtagcc gtcgtcggcg ccgccgcgcc
     1141 cgtacagctt gccgtctctg agcaccggct cccaagggcc gaggccgggc tcccagccgt
     1201 caaactccgg ctgcttgtcg tagtgaccgt acagcaggac ggtcccggcg tcgggcgccg
     1261 tggccggcac ctcgcagaac aacaccggcg tgcggccggg gatcgtgacg atttcggtgg
     1321 tgaggccgtc gatgtcgggc acggagcacc accctcgcag gagctcgacg gcggcctgca
     1381 tgtagccgtg ggcttcccag tcggcgtcga aagccggcga cttgttggga atacgaacgt
     1441 agtcgctcag cgtcgggatg atgcttcgat cccacagctc gttcacgaac ggggcgatgg
     1501 cgtcgctcat aggtctatgg ccacttgcag gtacacggca cgtcctggag atgcataacc
     1561 tagttggtcc tgatagctct cgtccagaag gttctcgccg cgaagcgcga gcttcagatg
     1621 ccggccgagg cgataagcca gatgggcgcg cagcagcacg tagtcgccca gccgaacccg
     1681 ctcggcgggg aagccgctga agtcgagatc ctcccggttg cccacgtagt agcccgacag
     1741 ctcggccgag aacggcccac gttcgtggct cacggtggcg taagcctgcc actgcggcct
     1801 gcgaatctct ctcgctgcgt ccggctcggc agcatccagc cagctcgcgc ccatgctcaa
     1861 gacgcccgtg ggtatcgcca gcgtggtctc tacctctacg ccgtcgcgtt tgctggtgcc
     1921 cggctcgttg atggaggtga acgcgccggc ggcgccgtcg aagtagaagc cgtttatctc
     1981 gtcttccagg cgatcgcgga aggcggtggc gcgcagggtc cattgccggt gcccgatcac
     2041 cgcccccgcc gacagatgcc tgttggtctc ggggtccaga tccggattgc cgagaaacgt
     2101 gtccggggtg aacccgaagc gttccacgaa gctcgggttc ttgatgccgg ttccccagtt
     2161 ggcccagagt tccactgccc ccgtcaggcg ataacgggca gccgcgcggt agctctcctt
     2221 gttctcgaag tcggagttgt cgtcggtgcg tgcggacagc gccagggaga gatcgtccag
     2281 gggcgcccaa aggtactcca gggcgaggct gttcgttcgc acggactgat tctgattcgg
     2341 atcaccgaag atcgagggcg cgccgcgctg ctcgaatcgc tccttttcgt gctccagcat
     2401 cagcgtgacc gcgtgacgcg agctcgcccg cagccgggtg agggaagtgg ctttgaatcg
     2461 ggccgcggcc gttctgcttt cccgggcacc gtcggcgaag ctgcgattgt cggtatcgaa
     2521 ataggagagg cgaatctgct gctcgaaccg ctcgtcgccg tgccaggtcg ccgcgacgct
     2581 gccgaggtac tcgtcgtgct tcgagacgcg atccccgtcg cgcggctgaa acgcaggaaa
     2641 cgggatggga tcgaagtccg cgtcgctctt caggtgatga acgagcgcct cgatggtcca
     2701 ggattcgctg cggtagccgc cgctcaagcg gtagctgctc tggtcgaagc cgtcgtcctc
     2761 ctcgccggtg gtggcgatgt tcgttccgtc ggttctgaat tcgctgagcg cgaggttgta
     2821 gtgatacgag cccgtcgtgt ccgcgagctg cagcgccgcc tggcgcgtgt cgaacgaccc
     2881 gccttccagg gacaacctgc gccggcgctc ggtgggcttc gtgatgaagt tcagcacgcc
     2941 ggccaccgcg tcgctccccc agatggcgct gtgggcaccg gccagcagct cgacgcgcgt
     3001 cgtgcccgtg agcgagaggt tggcgaagtt gaagctcgca tcggtggcag ggtctgccag
     3061 ctcgacgccg tccatgagga ccatgagatg gttggcttcg gatcctcgaa cgcgaacctg
     3121 ggtcacggag cccaggctgc cggcctggct gatggcgaga ctcggcaggc tgcgcaaggc
     3181 gtcggcgccc acggcgggcc cggtcccggt cgtgcgagtc aggacgggga gcaccggccg
     3241 gtccgtgcgt attcgatgcg cggttacggt gatggtctcc agctcggggt cgggctcggc
     3301 aaaggagagg ctcggggcga gcagggccag cactgtggcc aggaccatgg gcatgacggc
     3361 agactccggc ggcctgcctc accccgggca gggttgctga taccgggatc gggccggtct
     3421 ccggactcgc gagcatcaat ggcgtgcgcc gccttcccaa cgtttcagcg tcagtggctg
     3481 agcctgctca gtggcggctc cggcgcggcc gtctcgatta ccgttgcggg ggcagtgtcg
     3541 gatttccacc gacttcccgt tcacccgatc ccaccgcgcg gcaaccgcgc agcggcgcca
     3601 gtatagctac tcgaaccctt tgatgcagtc gtatccgtag agccagtcca tgcgctgcat
     3661 ggcgatctcc ggcaggaagc ggctggtcag cgacagtcgc agattgcggc gccaggcttt
     3721 cccgccgggg tccgtggtca gctctgccag gtgatgccgc tgtccctggc gaaagcggcc
     3781 gaggcgcacc ttccggtagc gctcgtagtc ggggaacccc ttggccggct cgtcctccca
     3841 gcgctcgagc atgcgcgaca gcacccaggc gtcttccatg gcaagcgacg cgccctgctg
     3901 caggtgcggg gtgacggggt ggcaggcgtc gcccaggagc gcgacccgcc ggtcgtacca
     3961 ctgggcgatg ggttcccgtt ccaccggggc agaccgggtg acgccgattg ccgagtccac
     4021 gagacggaag acgtcctcat gccagttccg gaaggcgccg gccagcttcg cccctgctcc
     4081 cgggtttcgg gcaaaggcaa ggaaacgcgc ggcgcagccg tcggcagacg gccactgggt
     4141 gaggtaggcg cggggtccga gccaggtggt gaccgcattg gcgcgcagac cgattggcag
     4201 gtcggtgccc gggcaggtgc ccgtccagac cacgttctgg gtcgtctgcg tcgtctcggc
     4261 gtatcccatg cccgcgcgaa cggctgaggc ctcgccatcg cagccgatga cgacgtcgtg
     4321 acagatctcc tgggagccga acgtcagcgt tac
//
LOCUS       DBNHQR010000428         3923 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_611072, whole genome shotgun sequence.
ACCESSION   DBNHQR010000428 DBNHQR010000000
VERSION     DBNHQR010000428.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_611072"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="AC16VR_09685"
     CDS             complement(<1..236)
                     /locus_tag="AC16VR_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HIR6011469.1"
                     /translation="MPATLPGWLGYIEGQHPQQIELGLDRVRAVATRLEVLKPAPVSI
                     IVAGTNGKGSTATCAEAVLLGAGLSVGATLSPHL"
     gene            complement(250..1053)
                     /gene="trpA"
                     /locus_tag="AC16VR_09690"
     CDS             complement(250..1053)
                     /gene="trpA"
                     /locus_tag="AC16VR_09690"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HIR6011470.1"
                     /translation="MSRLSRRLSALGGQGRKALVSFITAGDPDPSVTVPALHALVAGG
                     ADVLELGVPFSDPEAEGPVIQASSERALAKGTTLRAVLEMVARFRKDDDLTPIVLMGY
                     LNSVLAMGEETFAEAAAEAGVDGLIMVNLPPEEAGALRGLLKEKDIDLIFLVAPTTTP
                     ERAALITEQAGGFVYYVSLKGITGADNLETGSVSKQVGALKQMTELPVLVGFGIKDGG
                     TARAVAEKADGVVVGSALVRTMAGSADALAAIPALLTAQLAEIRRALDA"
     gene            complement(1050..2264)
                     /gene="trpB"
                     /locus_tag="AC16VR_09695"
     CDS             complement(1050..2264)
                     /gene="trpB"
                     /locus_tag="AC16VR_09695"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HIR6011471.1"
                     /translation="MSEGKGFNKYDQPVGGHFGQHGGRFVAETLMHALDELEALYERA
                     RKDPEFQAELAHDLEHFVGRPTPLYHAARLSRDLGGAQIYLKREDLNHTGAHKINNTV
                     GQALLAKRMGKPRVIAETGAGQHGVATATVAARLGLECCVYMGAEDVKRQSLNVYRMK
                     LLGAEVVPVNSGSRTLKDALNEAMRDWVTNVDTTHYIIGTVAGPHPYPMLVRDFQAVI
                     GQEARAQSLSMLGRLPDVLVACVGGGSNAIGLFYPFVDDVDVRMVGVEAGGLGLETGR
                     HAAPLSAGRVGVLHGNRTYLMEDDDGQIIETHSISAGLDYPGVGPEHSFLKDVGRAEY
                     DTVTDDEAMAGFRLLNRTEGIMPALETSHAIAWAARHARDFDRDAVMLINLSGRGDKD
                     ILTVAEIDGIEV"
     gene            complement(2242..2877)
                     /locus_tag="AC16VR_09700"
     CDS             complement(2242..2877)
                     /locus_tag="AC16VR_09700"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003104200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HIR6011472.1"
                     /translation="MPTKVKICGIARPEDARCVADAGADALGLIFVRQSPRFVSTGLA
                     REIAGEVAGSLSLIGLFVDADAAEVEAVLRDVDLDVLQFHGDEPGSYCEAFGVNYMKA
                     TRVSGPVDVAGLEAEYPGACCLLLDAYVPGVAGGTGVRLDLACWPEAGAKPLVLAGGL
                     TPENVADAISRVRPFGVDVSSGVEGGIKGRKDPERVRKFVSEVRRVGREGV"
     gene            complement(2930..3727)
                     /gene="truA"
                     /locus_tag="AC16VR_09705"
     CDS             complement(2930..3727)
                     /gene="truA"
                     /locus_tag="AC16VR_09705"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIR6011473.1"
                     /translation="MHLALGVEYDGSAFHGFQLQKAAATVQGALEQALSRIADHPLRI
                     AAAGRTDAGVHATGQVVGFASNAERPIQAWVKGVNALTPPSVKVRWAREVDPRFHPRY
                     SATARRYMYLWYEDDVTSPTLDRWAVRVRHLDDDAMHAASRSLVGEHDFSAFRASGCQ
                     SESAHRCVHRITVHRHGPLVVLDIAANAFLLHMVRNIAAALRDVGEGRRPAGWLAETL
                     AGRDRKHVGPTAPAHGLYLVGVRYPDQAFPPGEPPPLLRSLGGLARV"
     gene            complement(3727..>3923)
                     /locus_tag="AC16VR_09710"
     CDS             complement(3727..>3923)
                     /locus_tag="AC16VR_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003119743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FimV C-terminal domain-containing protein"
                     /protein_id="HIR6011474.1"
                     /translation="ATIDEDAFDFDVERDADSAETKLDLAKAYIDMGDEDGARDILKE
                     VLEEGTSDQQTKAQTLLEQL"
BASE COUNT          680 a         1332 c         1252 g          659 t
ORIGIN      
        1 tgcagatggg gggataacgt ggcccctacc gacagaccag ccccgagcag cacggcctcg
       61 gcgcaggtcg ctgtggaacc cttcccgttt gtcccggcga cgatgatcga gacgggtgca
      121 ggtttgagga cctccagccg ggtggcgacc gcccgcaccc tgtcgaggcc cagctcgatc
      181 tgttgcgggt gctgcccctc gatgtaccca agccaccctg gcagcgtggc gggcattccg
      241 gctggccggt caggcatcca gggcacgccg gatctcggcc agctgagcgg tgaggagagc
      301 gggtatggcg gccagggcgt ccgcgctgcc ggccattgtc ctgacgagag cgctcccaac
      361 aacgaccccg tcggctttct cggcaaccgc gcgagccgtc ccgccgtcct tgatgccaaa
      421 gccgaccagc acgggcagct cggtcatctg cttcagcgcc ccgacttgct tcgagaccga
      481 cccggtttcg aggttgtcgg cccccgtgat gcccttcagc gagacgtagt acacgaagcc
      541 gcctgcctgc tcggtaatca gcgccgcccg ctccggtgtc gtggtcgggg ccacgagaaa
      601 aatgaggtcg atgtcctttt ccttgagcag accgcgcaac gcgccggctt cttccggtgg
      661 caagttgacc atgatcaaac catcgacgcc tgcctcggca gccgcttcgg caaatgtttc
      721 ctcccccatg gcgagcacgg agttcaggta acccatgaga acgatcgggg taaggtcatc
      781 gtctttgcgg aatctggcca ccatctccag cacggcacgc aacgtggtcc ccttggcaag
      841 cgcccgctct gaggacgcct gaatcacggg cccttcggcc tccggatccg agaaaggcac
      901 gccgagttcc agcacgtcgg ctccgcccgc gaccagcgcg tgcagggccg gtaccgtgac
      961 tgacggatcg ggatcgccag cggttatgaa cgagaccagg gctttgcggc cctggccgcc
     1021 cagcgcgctc agccttcggg agagacggct cacacctcga tcccgtctat ctcggccacc
     1081 gtgaggatgt ccttgtctcc gcgcccggac aggttgatca gcatgacggc gtcacgatcg
     1141 aaatctcggg cgtgccgcgc tgcccaggct atggcgtgac tggtctccag cgcaggcatg
     1201 atgccctctg tccggttcag caggcgaaag ccggccatgg cttcgtcgtc cgtgacggta
     1261 tcgtactcgg cgcggccaac gtccttgagg aacgaatgct ccggcccgac gccggggtag
     1321 tcgagcccgg ccgatatgga atgcgtttcg atgatctgac catcatcgtc ttccatgagg
     1381 taggtgcggt tgccgtggag cacgcccacc cgcccggcgc tcaggggcgc cgcgtgcctg
     1441 ccggtctcga gacccaagcc gcccgcttcc acaccgacca tgcgtacgtc gacgtcgtcg
     1501 acgaacgggt agaaaagacc aatggcgttg gagccgcccc ccacgcaggc cacgaggacg
     1561 tcgggcagcc ggccgagcat gctcagagac tgagcccgcg cttcctgccc gatcaccgcc
     1621 tggaagtcac ggaccagcat gggataggga tggggtccgg ccaccgtgcc gatgatgtag
     1681 tgggtcgtat cgacgttggt cacccaatcc cgcatggcct cattgagcgc atccttcagg
     1741 gtgcgcgagc cggagttgac ggggaccacc tccgccccga gcagcttcat ccgatagacg
     1801 ttcaggctct gccttttgac atcttcggcc cccatgtaga cgcagcattc caggccgaga
     1861 cgggcagcga cggtcgccgt cgcgacgccg tgctggccag cgccggtctc ggcgatgacg
     1921 cgaggcttgc ccatgcgctt ggcgagaagc gcctggccga cggtgttgtt gatcttgtgg
     1981 gcgccggtat ggttgaggtc ctcgcgcttg aggtagatct gcgcgccacc cagatcccgg
     2041 gacagacgcg cggcatgata gagaggggtt ggccgtccaa cgaagtgctc gaggtcgtgc
     2101 gcgagctcgg cctgaaactc gggatccttg cgtgcgcgct cgtaaagggc ctccagctcg
     2161 tccagggcgt gcatgagcgt ctcggcgacg aagcggccgc cgtgctggcc gaagtgaccg
     2221 cccacgggct ggtcgtactt gttaaacccc ttcccttccg acacggcgca cctcgctgac
     2281 aaatttcctg acccgctccg ggtccttgcg gcccttgatc ccgccctcga cgccgctgct
     2341 gacgtccaca ccgaacggcc gcacacggct gatggcatcg gccacgttct ccggtgtcag
     2401 gccaccggcc agaaccagcg gcttggcacc agcttccggc cagcaggcta gatccaggcg
     2461 tacgcccgtg ccgccggcaa cccccgggac ataggcgtcc agtagcaggc agcaggcgcc
     2521 cggatactcg gcctcgaggc cggcgacgtc cacggggccg ctcacacgcg ttgccttcat
     2581 gtaattcacc ccgaacgcct cgcaatagga tccgggctcg tctccgtgaa actgaagcac
     2641 gtccaggtca acgtcccgaa ggacggcttc cacctcagca gcatccgcgt caacgaaaag
     2701 cccgatgagg ctgagcgacc cggccacctc ccctgcgatc tcacgagcga gcccggtgga
     2761 aacgaagcga gggctctgcc gcacgaagat caacccaagg gcgtcggcgc cggcgtccgc
     2821 aacgcaacgt gcgtcctcgg gccgcgcaat cccacaaatt tttaccttgg tcggcacgac
     2881 cggatccggg ggaaaagggg cgtagtctag cggagacgta actggccttc tagacgcgtg
     2941 cgagcccacc cagcgagcgc agcaagggtg ggggttcgcc gggaggaaag gcttgatccg
     3001 ggtagcgaac gccaacgagg tagagcccat gggcaggtgc cgtcggcccg acatgctttc
     3061 ggtctctgcc ggcaagggtc tcggccaacc acccagccgg ccggcgccct tcgccgacgt
     3121 cgcgaagagc cgccgcgatg ttgcgcacca tgtgcagaag aaacgcgttt gcggcgatgt
     3181 ccagcaccac caggggaccg tgacgatgga cggtgatccg atgcacacat cggtgagccg
     3241 actcggactg gcacccgctt gctctgaacg ccgagaaatc gtgctcgcca acgaggctgc
     3301 gcgacgccgc gtgcatggcg tcgtcatcga ggtgccgcac ccgcacggcc caccgatcca
     3361 gcgtcgggga ggtcacgtcg tcctcgtacc acagatacat gtagcgtcgc gccgtggccg
     3421 agtagcgcgg atggaatcga ggatcgactt cccgcgccca gcgcaccttg acggatggcg
     3481 gggtcagggc gttcacccct ttgacccagg cctgaatcgg ccgctcggca ttggaggcga
     3541 aaccgacgac ctgacccgtg gcgtgaaccc cggcatcggt gcgcccggcg gctgcaattc
     3601 tcagaggatg atcggcaata cgcgaaagcg cctgctccag ggcaccctga accgtagctg
     3661 ccgccttctg cagctggaag ccgtgaaagg cactgccgtc atactcgacg cccagcgcca
     3721 ggtgcatcag agctgttcga gcagcgtctg cgcttttgtc tgctggtcgc tggtaccttc
     3781 ctcgagaact tccttgagaa tgtcccgcgc gccgtcctcg tcacccatgt cgatgtaggc
     3841 cttcgcgaga tccagcttcg tctccgcgct gtcggcgtcg cgttcgacgt cgaaatcgaa
     3901 cgcatcctcg tcgatggtgg cga
//
LOCUS       DBNHQR010000429         2374 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_926863, whole genome shotgun sequence.
ACCESSION   DBNHQR010000429 DBNHQR010000000
VERSION     DBNHQR010000429.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_926863"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="AC16VR_09715"
     CDS             <1..733
                     /locus_tag="AC16VR_09715"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6011475.1"
                     /translation="GWGMACRVWDNTTACLQDAGVGVVAYDHRNCGRSDKDFRDVSIS
                     ALGDDVVALCDHLGLGGVVVNGWSLGGVVVTDAAAKLGDRLAGLILTAAASPRYTQAD
                     GFPYGGQPEDVTATVAALRADRVNFLRTLYYEGAYAKDVGEAVKNWCWQISLQASPGA
                     DASLGALADADYREAMTQIAAPALVFVGALDGVADPGIGRTAAELLPNGRLVEMADCG
                     HTPFLEDPEAYHRALLEFLGELGNG"
     gene            726..1739
                     /locus_tag="AC16VR_09720"
     CDS             726..1739
                     /locus_tag="AC16VR_09720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6011476.1"
                     /translation="MADRQVNFGLWYDFRNPPPSTVSFEVLYETCLEQIGWAETLGFD
                     SVWLTEHHFCEDGYTPSPLVLAAAIGQRTKSMRIGTNLMLLPLADPVRLAEDSAALAI
                     LTNGRFDLGVGLGYRQLEFDYFGRKLSHRPSLMEEGVDILRQAWSGEPIRIDGKRYQI
                     DGLTVGPVPAHPPRLFMGGMAEPAIARAARIGDGFLSTGGIGHDVYVKALQDQGKDRS
                     EGAICAGNWAIIAEDPEKEAAKVGEHVLYQTNQYVSWGAFGPPDQVPLFPDAETAIRE
                     GLYELWDADQAVAGLTAMLKEYPEIVDVHFWAQFPGEPVESGSDRIEYIANHVLPRVR
                     AAL"
     gene            1829..2281
                     /locus_tag="AC16VR_09725"
     CDS             1829..2281
                     /locus_tag="AC16VR_09725"
                     /inference="COORDINATES: protein motif:HMM:NF024844.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAS1-like dehydratase domain-containing protein"
                     /protein_id="HIR6011477.1"
                     /translation="MGLLSPELLSNIGKTEAPRTQVVTRRDIRKYAIATNQTRRKYLD
                     GDVAPPLFHVAFFWDVVELDELNADGVSVDKLLPKFPLEREMAGGLDIDYHSRIKAGD
                     TLVATRTLTDIYEKQGRSGPLIFYEVVMEVKREDGEPVLTQKTTRILR"
BASE COUNT          399 a          762 c          830 g          383 t
ORIGIN      
        1 cggatgggga atggcctgcc gggtatggga caacaccacg gcgtgccttc aggacgccgg
       61 cgtcggcgtg gttgcctacg accatcgcaa ctgcggacgc tctgacaagg acttccgcga
      121 cgtctccatc tcggcattag gcgacgacgt ggtggcgctc tgcgatcatc tcgggctggg
      181 tggggtggtc gtcaacggct ggtcccttgg cggggtggtg gtcaccgacg ccgcagccaa
      241 gctcggtgat cggctggctg gactgatcct cacagcggcc gcctcgcctc gctacaccca
      301 ggccgacggt tttccctatg gcggtcagcc ggaggacgtg accgccacgg tggccgcgct
      361 gcgtgcggac cgggtcaact tcctgcgtac gctttactac gaaggcgctt acgccaagga
      421 cgtgggcgaa gcggtcaaga actggtgctg gcagatctca ctccaggcga gcccaggggc
      481 ggacgcctcg ctcggtgctc tcgccgacgc cgactaccgg gaagccatga cacagattgc
      541 ggcgcccgcc ctggttttcg tgggcgccct ggacggggtt gccgatcccg gcatagggcg
      601 cacggcggca gagctactgc ccaacggccg gctcgtggag atggccgact gcgggcacac
      661 gccgttcctc gaggatcccg aggcatacca tcgggcgctg ctcgagtttc tgggagagct
      721 gggcaatggc tgatcggcag gtcaacttcg gtctctggta cgacttccgc aacccgccgc
      781 cgagcacggt gagcttcgag gtgctctacg agacgtgcct ggagcagatc ggctgggcgg
      841 agaccctcgg cttcgactcg gtctggctca cggagcatca tttctgcgag gacggctaca
      901 cgccctcgcc gctggtgctg gcggccgcca tcggtcagcg cacgaagtcc atgcgcatcg
      961 gcaccaacct gatgctgctc cctctggccg acccggtgcg gctggccgag gactcagcgg
     1021 cgttggccat cctcaccaac gggcgattcg acctgggcgt ggggctgggg taccggcagc
     1081 tcgagttcga ctacttcggc cgcaagctca gccatcggcc gagcctgatg gaggagggcg
     1141 tggacattct ccgccaggcc tggtcgggtg agcccatccg catcgacggc aagcgctacc
     1201 agatcgacgg cctcaccgtg ggtccggtgc ccgcgcaccc gccgcggctg ttcatggggg
     1261 gcatggcgga accggccatt gcccgcgcgg cccgcatcgg ggacggtttc ctctccaccg
     1321 gcgggatcgg ccacgacgtc tacgtcaagg cccttcagga ccagggcaag gaccgctccg
     1381 agggcgccat ctgcgccggc aactgggcga tcatcgctga ggacccggag aaggaggcgg
     1441 ccaaggtggg cgagcacgtc ctctaccaga cgaaccagta cgtctcctgg ggcgccttcg
     1501 gcccgccgga ccaggtgccg ctgttcccgg acgccgagac cgcgatacgg gaggggctct
     1561 acgagctgtg ggatgcggat caggcggtgg ccggcctcac cgcgatgctt aaggagtatc
     1621 cggagatcgt cgacgtgcac ttctgggcgc agtttcctgg cgagccagtg gagagcggca
     1681 gcgatcgcat cgagtacatc gcgaaccacg tgctgccacg ggttcgggcg gcgctgtgaa
     1741 gcgcaccggg ccgtatacaa acgactgacg caacgggtta agctgctggc tctcgccaga
     1801 gcgctgggca aacgggaggg agagccctgt gggattgttg agcccggagc ttctcagcaa
     1861 catcggcaaa accgaagcgc cgcgcaccca ggtggtcacg cggcgtgaca tccgcaagta
     1921 cgccatcgcc accaaccaga cccggcgtaa gtacctggac ggcgacgtgg cgccgcccct
     1981 tttccacgtg gccttcttct gggacgtggt ggagctggac gagctcaacg ccgatggcgt
     2041 ctcggtagac aagctgctgc cgaaatttcc cctggagcgg gaaatggcgg gtgggctgga
     2101 catcgactat cacagccgga tcaaggcggg tgatacgctg gtggcgaccc gcaccctcac
     2161 agacatctac gagaagcagg ggcgctccgg gccgctcatc ttctacgagg tggtgatgga
     2221 ggtgaagcgc gaggacgggg agccggtgct gacccagaag acgacgagga tcctgcgatg
     2281 agcggcccca ccatcgagtc cgtcagcgag ggcgacgccc tgccggggcg ggcgttcacg
     2341 tgcacggagg tggagctctt cctctacaac gccg
//
LOCUS       DBNHQR010000430         4516 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_547672, whole genome shotgun sequence.
ACCESSION   DBNHQR010000430 DBNHQR010000000
VERSION     DBNHQR010000430.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4516)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_547672"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="AC16VR_09730"
     CDS             <1..212
                     /locus_tag="AC16VR_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011478.1"
                     /translation="FLSTYGAHLVLLTRKAPGRFPPLGEIEESVRQDAERAALQKANE
                     TAIQAIVDTYEVERQPEPRTRAGAG"
     gene            209..1231
                     /locus_tag="AC16VR_09735"
     CDS             209..1231
                     /locus_tag="AC16VR_09735"
                     /inference="COORDINATES: protein motif:HMM:NF025167.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HIR6011479.1"
                     /translation="MRTLRSAVLGLWACCAALPAVGHDARPNYVQITETAPNSFNVAW
                     KVPASMPGNALPHPLLPDDCKPQQPPRWHTTGAEHAAQQEFICEQGLSGRVVGIEFPV
                     INPSLSTLYRVRLANGEEHLKILKPDETVWMVPDAEDRLSVAKEYTGLGIEHIWIGLD
                     HLLFVACLLFISRTPRRLLITITGFTVAHSITLALSALDLVRIPTPPVEAVIALSVVF
                     MAWEIVRGDDDSLAYRFPVAVSSSFGLLHGFGFAAVLRDIGLPQTELPTALLFFNVGV
                     EIGQILFVMALLIAFVALRPALGRALRAEPGGAVHWSYLTTPASYVIGTLASYWLIER
                     LAGFWG"
     gene            complement(1265..2533)
                     /locus_tag="AC16VR_09740"
     CDS             complement(1265..2533)
                     /locus_tag="AC16VR_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011480.1"
                     /translation="MKGATLLFAVLGITFTCVANAQTIEQIEEYERRIDEQQRQLDAL
                     RAELDVLKKVAGMPVSPDEEEATLEVAETLPDSAPESKPFLERRSDNGTFTLGGRLHR
                     VLMQVDDSASSTGFFMDSNQGPTMLRADVFAPLNGGWRLSGALEVGIQSNRPDRVTQD
                     NPSPGTDVTVREADVELHNDKVGTFSFGRGFAAAWVVPEIDLSGTVPSSLLAVGNLAP
                     GMRFVDRTNDELSDISVNQHFADTERLLLVDRLRYDSPRLGGGFQLSGTVAADSRWDA
                     ALRYYPSNDDWTIRAAATYQHKPFQDLDYRVELGLSARHNRTGLSLTTGLLFGDASGR
                     DASGYIVKAGWLTNLTSLGSTAFSADFSSGSDIRLPDDDSESVGFFVQQKLDAVGLDV
                     YGGYRRYKVDRPDFDLRPLKVVVLGAMYTF"
     gene            complement(2639..4048)
                     /locus_tag="AC16VR_09745"
     CDS             complement(2639..4048)
                     /locus_tag="AC16VR_09745"
                     /inference="COORDINATES: protein motif:HMM:NF019157.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HIR6011481.1"
                     /translation="MAPHCKVVGVIGTETNFELLLPENWNGKFVMGGGGGFVGSVQNT
                     SLLLGSLQKGYATVGTDAGHAGHPLNASWAYNNLERLVSFGHQAVHRTAVTAKALITD
                     FYDEGISRSYFTGCSRGGGQGFMEAQRYPEDFDGIVAGSPAYDFLGVAAAATQISAAM
                     YPDPSNLQEAVVGPQEQRLIESSYLAMCDAMDGLEDGILNDPRQCQFDVATLLCEGEK
                     TDSCLSEEQLAAVRVVYDGPKDSQGRSLYYGFPFGGEGAEGGWSRWLTGGLKYDDDAD
                     DFQSGIDMGPFESPVTPSAYFSFGKEIMRYLIYNDPEWSYVGYDYDNLAKDGARAAET
                     LNATDPDLSAFRERGGKMLIYNGWSDNAQTSLAFADYYEQVLAHDESAADDVRMFLMP
                     GVEHCIGGPGPSWVNFLDEIDAWVESGKAPDQVVAYWLDETMQPAGSRPVCAYPAVAQ
                     YDGQGDPRDASSFSCSGGG"
BASE COUNT          848 a         1526 c         1328 g          814 t
ORIGIN      
        1 catttctgtc aacctacggc gcccacctgg tcctgctgac ccgcaaagcg cccggccgct
       61 ttcccccgct cggtgagatc gaagaaagcg ttcggcagga cgccgagcga gccgcgttgc
      121 agaaggcgaa cgaaaccgcc attcaggcca tcgtggacac ctacgaggtg gagcggcagc
      181 ccgagccccg cacgcgcgcc ggcgcaggat gagaactctc cgctcagccg tcctgggtct
      241 gtgggcgtgc tgcgctgcgc tgcccgccgt cggccacgat gcgcggccga actacgtcca
      301 gatcacggag acggcgccca atagcttcaa cgtggcgtgg aaggttcctg ccagcatgcc
      361 gggcaacgcg ctgccccatc ctctgctgcc ggacgactgc aagccgcagc agcctcccag
      421 gtggcacacc accggggcag agcacgcggc gcaacaggaa ttcatctgcg agcagggcct
      481 ctccggacgg gtcgtgggca tcgagtttcc cgtcatcaac ccttccctct ccacgctgta
      541 ccgcgttcgg ctggcaaacg gcgaagagca tctgaagatc ctgaagcccg acgagacggt
      601 gtggatggtt cccgatgcgg aggaccgtct ctcggtcgcg aaggagtaca cggggctcgg
      661 gatcgagcac atctggatcg gcctcgacca cctcctgttc gtcgcctgcc tgctgttcat
      721 ctcgcgcacg ccccgacgcc tgctcatcac catcacgggc ttcaccgtgg cgcactccat
      781 caccctggcg ctgtcagctc tcgacctggt gcgcatcccc acgccgccgg tggaagccgt
      841 catcgcgctc agcgtggtgt tcatggcctg ggagattgtg cgcggcgacg acgactcttt
      901 ggcctaccgt tttccggtcg ccgtttccag ctccttcggt ctcctccacg gcttcggttt
      961 cgccgccgtg ctgcgcgaca ttggcctgcc ccagacggag ctgcccaccg cgctgctgtt
     1021 cttcaacgtg ggcgtggaga tcggccagat actcttcgtg atggcgctgc tgatcgcctt
     1081 cgtagcgctg cggccggccc ttgggcgtgc gctgcgcgct gagcccggcg gagccgttca
     1141 ctggtcgtac ctgaccaccc cggcaagcta cgtcatcggc accctggcga gctactggct
     1201 gatcgagcgc cttgctggct tctgggggtg atcgcgcacc ccgcggattg atcgctcgcc
     1261 cctgtcagaa cgtgtacatc gcccccagca cgacgacctt cagcggccgc agatcgaaat
     1321 caggccggtc caccttatag cggcgataac ctccgtagac gtccagccca acggcgtcca
     1381 gcttctgctg gacgaaaaag ccaacggatt ccgaatcgtc gtcagggagg cgaatatcgc
     1441 tgcccgatga aaagtcggcg gaaaacgccg tcgatccgag actcgtgagg ttggtgagcc
     1501 agcccgcctt gacgatgtaa ccggacgcat cccgccccga agcgtcgccg aacaacaggc
     1561 ccgtggtcag gctcagaccc gtccggttgt gccgcgcgga aaggcccaat tcaactcgat
     1621 agtcgaggtc ttggaacggt ttgtgctgat aggtagcagc cgcgcgtatg gtccagtcgt
     1681 cgttcgacgg gtagtagcgg agcgccgcat cccagcgcga gtcggcggcg acggtccccg
     1741 acagctgaaa gccgccacca aggcggggcg agtcataacg cagacggtcg acgagcagca
     1801 gccgctccgt atcggcaaag tgctgattga cgctgatgtc gctcagctcg tcgttcgtcc
     1861 ggtcgacgaa ccgcatgccg ggtgcaaggt tgccgacggc aagcagcgag gacgggaccg
     1921 tgccggaaag atcgatctcg ggaaccaccc aggcagccgc gaagccgcgc ccgaaactga
     1981 acgtgcccac tttgtcgttg tgcagctcca cgtccgcttc tctgacggta acgtccgtac
     2041 ccggactcgg attgtcctgg gtcacgcggt cgggccggtt gctctggatt cccacctcga
     2101 gagcgccgga caaccgccat ccgccgttca gcggcgcaaa cacgtccgcc cgcagcatgg
     2161 tcggcccctg attcgaatcc atgaaaaacc cggtgctcga tgcgctgtca tcgacctgca
     2221 tgagcacccg atgcaggcgc ccgcccaagg tgaacgtacc gttgtcgctg cggcgctcga
     2281 ggaacggctt cgattccggc gcggaatccg gcagcgtttc ggccacctcc agcgttgcct
     2341 cctcctcatc cggcgaaacc ggcatgccag cgactttttt gagtacgtcg agctcggcgc
     2401 gcagggcgtc gagctgccgc tgctgctcgt cgatgcgccg ctcgtactcc tcaatctgct
     2461 caatcgtttg cgcgttcgcg acacaggtaa acgtgatccc cagcaccgcg aacaaaagtg
     2521 tcgcgccctt catgatgatt cccctcaggc tgcccaggcg atgaagcaag ctcggcgcaa
     2581 tggtcaacac cccattgcgc cgacgagttc catatgacct gattttgtcg gctatttgtc
     2641 agccgccccc ggaacagctg aaactcgacg catcccgcgg atcaccctga ccgtcgtact
     2701 gcgcgactgc agggtaggca cagacaggcc tcgatccggc cggctgcatg gtctcgtcga
     2761 gccagtaggc gacgacctgg tccggggcct tgcctgactc gacccaggcg tcaatctcat
     2821 cgaggaaatt gacccaggag ggccctggtc cgccgatgca atgctccacg cccggcatca
     2881 ggaacatacg cacgtcatcc gcagccgact catcgtgcgc aaggacctgc tcatagtagt
     2941 cggcaaacgc gagagatgtc tgcgcattgt ccgaccagcc gttgtaaatc agcattttgc
     3001 cgccgcgctc gcgaaacgcc gacaggtccg gatcggtggc attgagcgtc tcggcagcac
     3061 gggccccgtc cttggcaagg ttgtcatagt cgtaaccgac gtaactccac tcaggatcgt
     3121 tgtagatcaa ataccgcatg atctccttgc caaatgagaa ataggcactc ggcgtcacgg
     3181 gcgattcaaa tggccccatg tctataccgg actgaaagtc atcggcgtcg tcgtcgtact
     3241 tcagcccgcc ggtgagccag cgggaccacc ccccctcggc gccttcacca ccaaacggaa
     3301 atccgtagta caaggaccgt ccctgggaat ccttgggccc gtcgtaaacg accctgaccg
     3361 ccgcaagctg ctcctcgctg aggcagctgt cggtcttctc cccttcgcac agcaaagttg
     3421 ccacatcgaa ctggcactgt cgggggtcgt tgaggatccc gtcttccaaa ccgtccatcg
     3481 catcgcacat ggcgaggtac gacgactcga tcaaacgctg ctcctgcggg cccaccacgg
     3541 cttcctgcag gttggaagga tcggggtaca ttgccgcgct gatctgggtg gccgccgcag
     3601 caacccccag aaaatcgtat gccggtgagc cggccacgat gccgtcgaaa tcctccggat
     3661 accgctgagc ctccatgaag ccctgccccc ctccgcgaga gcatccggtg aagtagctgc
     3721 ggctgatgcc ctcgtcgtaa aagtcggtga tcagcgcctt cgccgttacc gcggtgcggt
     3781 ggacggcctg gtggccaaag ctgacgaggc gctccaggtt gttgtaggcc cagctcgcat
     3841 tgagcggatg ccccgcatgg ccggcgtcgg tgccgaccgt tgcatagcct ttttgcaggg
     3901 atccgagcaa aagcgaggtg ttttgcacgc ttccgacgaa cccaccgccg ccgcccatga
     3961 cgaacttgcc gttccagttc tccggcagca gaagctcgaa attggtctcg gtaccgatca
     4021 cgccgaccac cttgcaatgc ggcgccatcg aggcctcctg cgtgaccgag atgagccgga
     4081 cgtcgggaag tgccggaaac gatgcctgag aacagaccgg acccgaggtc gccggcaggc
     4141 tctgctccga ggttccgcac gccgtgagca cggctgtcag cagaattaac aagcctacat
     4201 ctaatggcct ttcaaacatc atttcgtccc cttccactct cgatcgcaat cagccagatc
     4261 ctcaggtaag gcaacagaaa cctcaacccc cgaggcagtt tccgaccggc actgccgatc
     4321 cgacgatgtt cacaagcata ttcacctgct ccgccattcg cttatcattt gacgccaccc
     4381 tcgcaaaggc gctgagcaag agggaaccat gagcgagacc agcaggcact gcagcaagcc
     4441 acaggtgcca attatccgtg cggccaacct gatgggcttc atcgactttc tggagaaaac
     4501 cgccgggcca gcggcc
//
LOCUS       DBNHQR010000431         1233 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_926902, whole genome shotgun sequence.
ACCESSION   DBNHQR010000431 DBNHQR010000000
VERSION     DBNHQR010000431.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1233)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_926902"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="AC16VR_09750"
     CDS             <1..357
                     /locus_tag="AC16VR_09750"
                     /inference="COORDINATES: protein motif:HMM:NF047238.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase C-terminal domain-containing protein"
                     /protein_id="HIR6011482.1"
                     /translation="FEIAGIGPEDVDLAQLQDTESGAEVMHMAENGFCEHGEQEHIIQ
                     NGETEIGGRFPVNTDGGCLANGEPVGASGLRQVYENVLQLRGGAGERQVPGNPKVAYT
                     HVYGAPGISGVNILTT"
     gene            462..>1233
                     /locus_tag="AC16VR_09755"
     CDS             462..>1233
                     /locus_tag="AC16VR_09755"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIR6011483.1"
                     /translation="MSDTNRQFLLAARPVGMVKESDLQLHEAPVPEPGEGEVLVRVEY
                     ISLDPSMRGQMENRADYVAPLALGDVMRARGVGRVVRSNDPEFPVGAVVSGTFGMQEY
                     AIGSDRVDRFRRWDDAVDPKVVLGTLGGTGLTAYFGLLDVGEPKSGDMVVVSGAAGAT
                     GSVAGQIAKIKGCTVIGMAGTDEKCRWLTDELGFDGAINYKTQDVAAELDRLCPDGID
                     VYFDNVGGEILDICLDRIALNARVVLCGGISRYNVEGEL"
BASE COUNT          213 a          384 c          449 g          187 t
ORIGIN      
        1 ttcgagatcg ccggcatcgg tccggaagac gtggacctgg cccagctgca ggacacggag
       61 tccggcgccg aggtcatgca catggcggaa aacggcttct gcgagcatgg cgagcaggag
      121 cacatcattc agaacggcga aacggagatc ggcgggcggt tcccggtgaa caccgacggc
      181 ggctgcctgg ccaacggcga gccggtgggg gcctcgggtc tgcgccaggt ttacgagaac
      241 gttcttcagc tgcgcggcgg tgcaggcgag cggcaggtgc ccggcaatcc caaggtcgcc
      301 tatacccacg tgtacggcgc gcccggcatc agcggggtga acatcctcac gacgtagtcg
      361 aaagatgact gtcctcaacg cgcacgacgc gctcttaagc gcccgcgcca cgggccatcc
      421 ggggcagcac cgtctgccct ggaaaccgtg ggagggcgct gatgagtgac acgaaccggc
      481 agttcctgct ggccgcccgt cccgtgggca tggtcaagga gtcggatctg cagctccacg
      541 aggcacccgt tccggagccc ggcgaggggg aggtgctggt gagggtggag tacatctctc
      601 tggacccctc catgcgcggg cagatggaga accgcgccga ctacgtcgcc ccgctcgctc
      661 tcggtgacgt catgcgggcc cggggggttg gccgggtggt gcggtccaac gatccggagt
      721 tcccggtggg cgctgtggtg agcggcacgt tcgggatgca ggagtacgcc atcggcagtg
      781 acagggtgga ccgtttccgc cgctgggacg acgctgtgga ccccaaggtg gtgctgggga
      841 ccctcggtgg cacagggctc acggcctact tcggcctgct tgacgtgggc gagccgaagt
      901 ctggagacat ggtggtcgtc agcggcgcgg ccggcgccac cggcagcgtg gcgggccaga
      961 tcgcgaagat caaaggctgc accgtcatcg gcatggcggg cacggacgag aaatgccgat
     1021 ggctcaccga cgagctcggc ttcgacggcg ccatcaacta caagactcag gacgtggccg
     1081 cagagctcga ccggctgtgc ccggacggca tcgacgtcta cttcgacaac gtgggcggcg
     1141 agatcctcga catctgcctg gaccggatcg cgctcaacgc ccgggtcgtg ctctgcggcg
     1201 gtatctcccg ctacaacgtg gaaggagagc tgc
//
LOCUS       DBNHQR010000432         2661 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_484219, whole genome shotgun sequence.
ACCESSION   DBNHQR010000432 DBNHQR010000000
VERSION     DBNHQR010000432.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_484219"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="AC16VR_09760"
     CDS             <1..308
                     /locus_tag="AC16VR_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011484.1"
                     /translation="RIPANALEHCLELLVALDVLAETDEPKPSYSPALPLDTTTVAEV
                     LQRVETYQPPGTYAPALGADEDIVTIEAAGGSGRDRALGGLTLKQLAAGDSSEVVTA"
     gene            630..>2661
                     /locus_tag="AC16VR_09765"
     CDS             630..>2661
                     /locus_tag="AC16VR_09765"
                     /inference="COORDINATES: protein motif:HMM:NF013117.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR6011485.1"
                     /translation="MTSLDRISGEINELGQLADDAAELDALQIQTDELISITENIDGR
                     GADASFDGSLVKARALAVRAEAVTTALSDHALAMEAKLEEIRQTTAQWQRIQGEITDI
                     PEAISQRIARILATGAGLENRINAKLNETVELQNEALGVLDVARSLQERFDSYGRSRR
                     GQLLEANAAPVWLMKSEHLSSSSEKAGRDFVGTLRRDYATWVETSQAIIGGHFLLLPL
                     LLFLLTKLKATALQSPTGALARPTSTGLLIWMLLGVAIYAGAPVAVRTVFVAIASIVT
                     GFVLVGFLDKRLRAGAIAFLILNVVEDLISGFRVTDALPRLVYLLLALGAIVLVMLAR
                     RPHTLSAMLAWRMPRPILVSGMWTGIALLVLSCIANVAGYVYLSQVLVNGVLASTGVL
                     LVLFAGLTSVSEIIVAVLSLDALNNVRSIANHRYRLNRALRRPLVVLAMFLWAWATTR
                     AFGIDDWVIASIGAIVGAEMSIGSVTLSLQGVLVFVIAVLSAVWASRIVRAVLNYDVL
                     PRMQLPRGVPNTISMTLHYSIILIGLLLGVGFMGIDLSSLAFIVGALGVGIGFGLQNL
                     VNNFVSGLILIFEAPIQVGDTVEVGPLMGKVVQIGIRTSRVRTFGGSEVIVPNGDLVS
                     NQVINWTLSDRSRRLELEVGVAYGSDPERVTEVLRSVVDGDEDVLEDPAPIIM"
BASE COUNT          460 a          884 c          838 g          479 t
ORIGIN      
        1 atcgcatccc agccaatgcc ctggagcact gcctcgagct tctcgtggcg ctggacgtgc
       61 tcgcggagac ggacgagccc aagccttcct acagtcccgc ccttcccctg gacaccacca
      121 ccgtcgccga ggtgcttcag cgggtggaga cataccagcc acccggaacc tacgcgcccg
      181 ccctgggcgc tgacgaggac atcgtgacga tcgaggcagc cggcggttcc ggcagagatc
      241 gtgcgctcgg cgggctcacc ctcaagcagc tggcagccgg cgattcgagc gaagtcgtca
      301 cggcctgatc aggatcctcc cgccctggaa ggagcccccg cgcgatcgcg attggcccgt
      361 ggatcggctc gtcaacgtgc gcgcctgggt accggttgag accaggaagc ttcgccccta
      421 aacttcgcca ctttccccaa agggccatgg tgatgcgtcg atcgctgggt cgccacagat
      481 tgatcatggc gtcgtgcttc atctggctcg ctagttggcc cggccacgcg tcagcgcagc
      541 agaccacaag cccggatgcc gccggatccc aggggacgcc cgcggaagtc cagccaacca
      601 cggacctcac cagccgcacc caggcaacgt tgacgtccct ggatcgcatc tcaggagaaa
      661 tcaacgagct gggccagctc gccgacgacg ccgcagagct ggacgccctg cagattcaga
      721 ccgatgagct gatcagcatc accgaaaaca tcgacgggag aggcgcagac gcctcgttcg
      781 acggttcgct ggtgaaagcc cgagcgctcg ccgtcagagc agaggcggta accaccgccc
      841 tgtcggacca cgctctcgcc atggaagcca aactcgaaga gatccgccag acgacggccc
      901 agtggcagcg gatacagggc gagatcacgg acattccgga agccatcagc cagcgtatcg
      961 cgaggatcct ggcgaccggt gcaggcctgg agaaccggat aaacgccaag ctgaacgaaa
     1021 ccgtggagct ccagaacgag gcgctcgggg tgctcgacgt cgcccggtcg ctgcaggaac
     1081 gcttcgacag ctacgggcgg tcccggcgcg ggcagctgct ggaagcgaat gcggccccgg
     1141 tctggctgat gaaatccgaa catctcagca gctcttcgga gaaagcgggt cgggacttcg
     1201 tcggcacgct gcgtcgcgac tacgcaacct gggtagaaac ctcccaggcg atcatcggcg
     1261 gtcacttcct cctgcttcca ctgctgctgt tcctgcttac gaagctcaag gcgacggcac
     1321 tgcaatcccc gacgggcgct ctggcccgcc ccacgtcgac cgggctcctc atctggatgc
     1381 ttttgggcgt ggcgatctac gccggggcgc cagtcgccgt gcgtaccgtc ttcgtggcga
     1441 ttgcctcgat cgtgaccggg ttcgtcctcg tcggtttcct cgacaaaaga ctgcgagccg
     1501 gggcgatagc attcctgata ctgaacgtgg tggaggatct gatcagcggc ttccgcgtga
     1561 ccgacgcgct gccacgtctc gtctacctgc tccttgcctt gggcgccatc gttctcgtga
     1621 tgctggcccg acgaccccat acgttgagcg ccatgctcgc ctggcgcatg ccccgtccga
     1681 tcctggtcag cgggatgtgg acgggcatcg cccttctggt gctcagctgc atcgccaacg
     1741 tcgccgggta cgtgtacttg tcgcaggtcc tggtcaacgg cgtcctggcc agcaccgggg
     1801 tgctgctggt gctgttcgcg gggctcacgt cggtctccga gatcatcgtt gccgtgctga
     1861 gcctggacgc actgaacaac gtacggtcaa tcgccaatca ccgctatcgc ctcaaccggg
     1921 cgttgcgcag accgctcgtt gtgctggcca tgttcctgtg ggcatgggca acgacccgcg
     1981 cgttcggcat cgatgactgg gtcatcgcct ccatcggcgc gatcgtcggc gccgagatgt
     2041 cgatcggctc ggttacgctg tcattgcagg gggtgctggt cttcgtcatc gcggtgctgt
     2101 ccgccgtctg ggcctcgcgg atcgtccggg ccgttctcaa ctatgacgtt ctcccccgaa
     2161 tgcagctgcc ccgcggggtt cccaacacca tatcgatgac gctgcactac tcgatcatcc
     2221 tgatcggcct gctgctcggc gtgggcttca tgggcatcga cctcagcagc ctggcattca
     2281 tcgtgggcgc gctcggcgtg ggtatcggct tcggcctgca gaacctggtc aacaacttcg
     2341 tctcgggcct gatcctgatc ttcgaagccc ccatacaggt gggcgatacg gtggaagtcg
     2401 gtccgctgat ggggaaagtg gtgcagatag gcatccggac gagcagggtt cgcacgttcg
     2461 gaggttcgga ggtgatcgtg cccaacggcg acctcgtctc caaccaggtc atcaactgga
     2521 cgctctcgga ccgcagccga cgtctggaac tcgaggtcgg cgtggcctac ggcagcgacc
     2581 cggaacgggt caccgaagtg ttgcgcagcg tcgtggacgg cgacgaggac gtcctcgaag
     2641 accccgcacc gatcatcatg t
//
LOCUS       DBNHQR010000433         2069 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1180052, whole genome shotgun sequence.
ACCESSION   DBNHQR010000433 DBNHQR010000000
VERSION     DBNHQR010000433.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1180052"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="AC16VR_09770"
     CDS             complement(<1..305)
                     /locus_tag="AC16VR_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011486.1"
                     /translation="MKALKYVLLVVLVAVIGAVAYVVVNAGSIVKQVIETVGSDVLGV
                     PVTVRSVDLSLDGGSIGGLQISNPEGFETPYALRVETAAVKINAQASTTKLVVIDQIL
                     "
     gene            410..1522
                     /locus_tag="AC16VR_09775"
     CDS             410..1522
                     /locus_tag="AC16VR_09775"
                     /inference="COORDINATES: protein motif:HMM:NF012335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR6011487.1"
                     /translation="MAGINRAALFFEPGKPFEIVELPIPEPAAGGLTLKVTRANICGS
                     ELHMWRGDGRFAQVITPKGRILGHEATGVVHALGEGVTTDWAGNALAEGDRIAYQYFQ
                     PCGRCRNCTRGMSEACRDSFSIQRGSLEEWPYTRGSFADYLYLHPRQAIFKVPDHVTD
                     TMVVSANCALAQVVMGLERVNVQLGDRVVVQGCGGLGVYACAIAKERGAELVIAIDGI
                     DERLELAQRMGADHVIDLRQVEDSKARVGAVKDLTGGLGADVVVEVVGFASVINEGLR
                     MLAWGGRYLEMGTFYTGTSFECDPGRLVGQNQRIEAVASYDAASLQRAIEFLARNGDR
                     LPLDEVVVDYPLDQIERAFADQDSGLVRRASLVMGD"
     gene            1530..>2069
                     /locus_tag="AC16VR_09780"
     CDS             1530..>2069
                     /locus_tag="AC16VR_09780"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6011488.1"
                     /translation="MTEYDVIIIGAGMSGMYQLYRLRQLGLSVRVYEAGSGVGGTWYW
                     NRYPGARFDSESYSYGYSFSQELLDEWDWSEHFSAQPETLRYLNHVADKFDLRRDMQF
                     DSRVVRATYDDAAPGWEIELESGERARAWLLISAVGPLSAPIMPDIPGIDDFRGEAYH
                     TGLWPHEPVSFAGKRVAVIG"
BASE COUNT          333 a          642 c          735 g          359 t
ORIGIN      
        1 aggatctggt cgatgaccac gagcttcgtg gtgcttgcct gggcgttgat cttcaccgcg
       61 gctgtttcca ccctgagggc gtagggggtt tcgaacccct cggggttgga gatctgcaag
      121 ccgccgatgc tgccgccgtc cagggagagg tcgacggacc ggacggtgac cggaacgccg
      181 agcacgtccg accccaccgt ctcgatgacc tgcttgacga tgcttcccgc gttgaccacc
      241 acgtaggcga cggcgccgat cactgcaacc agcacgacca gcaggacgta cttcaatgcc
      301 ttcatcgatc ctcccctttg ctctaggatg cgccaggagg gcgcgtttct gggggcacgt
      361 tgcctttaaa cgctggcggt ttcaaggttt tcagactggg gaggtagaca tggcgggcat
      421 caacagggcg gcgctctttt tcgagccggg caaaccgttc gagatcgtcg agctgccgat
      481 tcccgagccg gcggccgggg ggctgacgct caaggtaacc cgggccaaca tctgcggatc
      541 cgagctgcac atgtggcggg gtgacggccg cttcgcccag gtcatcacgc ctaaggggcg
      601 cattctgggc cacgaagcca ccggtgtggt tcacgccctt ggcgaggggg tcacgaccga
      661 ctgggcgggc aacgcgctcg cagaagggga ccggatcgcc tatcagtact ttcagccctg
      721 cggtcgctgc cgcaactgca cccggggcat gagcgaggcc tgccgggaca gcttcagcat
      781 ccagcgcggc agcctggagg agtggcctta cacccgcggg tcgtttgccg actacctgta
      841 cctgcatccg cgccaggcca tcttcaaggt gccggaccac gtcaccgaca ccatggtggt
      901 gagtgcgaac tgcgcgctgg cccaggtggt catgggtctg gaacgtgtca acgtgcagct
      961 gggcgaccgg gtggtggttc agggctgcgg ggggctgggc gtttatgcct gcgcgatcgc
     1021 caaggagcgg ggcgcggagc tcgtgatcgc catcgacggc atcgacgagc gtctggagct
     1081 cgctcagcgc atgggcgcgg atcacgtcat cgatctgcgt caggtggagg actccaaagc
     1141 cagggtgggc gccgtcaagg acctcaccgg cgggctcggc gcggacgtgg tcgtcgaggt
     1201 ggtggggttt gccagcgtga tcaacgaagg cttgaggatg ctcgcctggg gcggccgtta
     1261 cctggaaatg ggcacgttct acacaggcac gagcttcgag tgcgaccccg gcaggctcgt
     1321 cgggcagaac cagcgcatcg aggcagtggc ctcctacgac gcggcaagcc tgcagcgcgc
     1381 catcgagttc ctcgcccgca acggggaccg cctgcccctg gacgaggtgg tggtggacta
     1441 cccgctggat cagatcgagc gggcctttgc ggaccaggat tcggggctcg tccggcgcgc
     1501 gtccctcgtg atgggggatt gatgcgacga tgaccgagta cgacgtcatc atcatcggcg
     1561 ccggcatgtc cgggatgtac cagctgtacc ggctgcggca gctgggtctg tcggtgcgcg
     1621 tctacgaggc gggcagcggc gtgggcggca cgtggtactg gaaccgctat ccgggtgcgc
     1681 ggttcgactc ggagagctac agctacgggt actcgttctc ccaggagctc ctggacgagt
     1741 gggactggag cgagcacttc tcggcccagc ccgagaccct gcgctacctg aatcacgttg
     1801 cggacaagtt cgacctgcgg cgtgacatgc agttcgacag ccgggtcgtg cgggccacct
     1861 acgacgacgc tgcgccgggt tgggagatcg agctcgagag cggcgagcgg gcgcgagcct
     1921 ggctcctcat cagcgccgtt ggcccgctgt cggcgccgat catgcccgat atccccggca
     1981 tcgacgattt ccgcggcgag gcgtaccaca cggggctgtg gccccacgag ccggtgagct
     2041 tcgccggcaa gcgggttgcc gtcatcggc
//
LOCUS       DBNHQR010000434         5388 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_991473, whole genome shotgun sequence.
ACCESSION   DBNHQR010000434 DBNHQR010000000
VERSION     DBNHQR010000434.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5388)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5388)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5388
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_991473"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..1245)
                     /gene="solA"
                     /locus_tag="AC16VR_09785"
     CDS             complement(79..1245)
                     /gene="solA"
                     /locus_tag="AC16VR_09785"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF008425.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HIR6011489.1"
                     /translation="MSYDVIVLGVGAMGAAALERLASRGARVLGIEQWSVPNSLGSSG
                     GDTRLIRKAYFEHPDYVPLLERAYEGWRRLEALSGLELLYPTGTVYVGPPDGPLIGGS
                     RMAAESHRLEFEPLAPAELHDRFPGFRLPDGCVSAFEPQAGFVLAGHGINAFAKLAIA
                     NGATLMSQTRVDGWRAESGGVVVSTSAGELEAGALVIAAGSWSSDICRLPRVALNVTR
                     QTLFWIWPERCAEFSLGRFPCWALEIAGHPGIYYGFPACVPNTARVGAKFAHHAPGET
                     CEPDGPKAPPSEAEYQATLRAVRSLLPGLRGSRIGSSICMYTNSRDGHFIVDRHPDHD
                     AVTVACGFSGHGFKFAPVMGELLADYALEGTSRLPAEFLRIGTRDLWHADEPKP"
     gene            1244..2437
                     /locus_tag="AC16VR_09790"
     CDS             1244..2437
                     /locus_tag="AC16VR_09790"
                     /inference="COORDINATES: protein motif:HMM:NF016214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HIR6011490.1"
                     /translation="MAQSYLSSAKRAEGTVHIAAANRLHRRYDARNCSSRRVTSVDPV
                     SQAALGAVVARSVSHRQLGAGAVALGAAAGALPDIDVFFSLDGDYFDQLVSHRGITHS
                     LFFAPVVGPLFGWLAWRWTARRRNDPGEPAALRAWMLAMTLAMLSHPLLDLLTPYGTQ
                     LLQPFSDARFAVAAMPIIDPIYTIVLLAGLVAGYRFTAAGGRIAAATLLVSCAYLGYG
                     WHLNEGAERFARAQLAAQGLPNVEVAAFPTILQIHHRRVVARTPDADRVGFLSMWDPC
                     DIGWQREPRLPRSQVEAFLRTREGAIFDWFTMGWSHYAIQDGHMTATDLRYGSDENPR
                     ASVFTASVALDHLGRPSGNIAGGRFTPDANGRNLERLIAMTYAPGCGTSPGRMAQTSL
                     EAGAG"
     gene            2502..4010
                     /gene="nhaC"
                     /locus_tag="AC16VR_09795"
     CDS             2502..4010
                     /gene="nhaC"
                     /locus_tag="AC16VR_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HIR6011491.1"
                     /translation="MDDDSLTMEQEAGRPNDPSNLDVVVAIVPTTVLLALSVALFGSD
                     SSSGANQIALVIGAALATMVGVKNGHSWKVIEENIVGGISTAMPAIMILLSVGSLIGV
                     WMLSGTVPTMIYYGLLLLEPAVFYAASCLLCAVTALSIGSSWTVAGTLGIGLIGVAAG
                     LELSLPITAGAIISGAYFGDKLSPLSDTTNLAPAVTEIDIFTHIRHMTWTTLPSFVFA
                     LGAFTAIGLMESTPETQTSLRVTLETLDEAFAINPLMLLPVALVVLLAGRKKPAVACI
                     LAGVALGILMALVYQRPAIVRFADDASLGPTVAALKAIWIVLFDGYAGSTGEANLDEL
                     LSRGGMSSMLNTVWLILAAMTFGAAMEQAGVLRRLVHALASAARSTGNLIATTLVTCI
                     GVNVLAADQYMSIVLTGRMFRSEYRRRGLHEKNLARTLEDGGTITSPLVPWNTCGAYM
                     AATLGVPTLAYLPFCFFNLANPFVSAAYGYAGFKIEPLKPGGEGEAAPSRAQ"
     gene            complement(4035..5225)
                     /locus_tag="AC16VR_09800"
     CDS             complement(4035..5225)
                     /locus_tag="AC16VR_09800"
                     /inference="COORDINATES: protein motif:HMM:NF014244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR6011492.1"
                     /translation="MSPLLPSAGGGLKLFSIFGIEIRLDYSVAIIFGLVVYSLGGGLF
                     PQWHPDWPPSTAWLTALVAGVLFFASLLAHELSHSLTARRYGIRVPRITLFLFGGVAE
                     IESEAQTPSAELTIAIAGPLMSALLGAVFTVVAFGSMDTLAREAFAEDPETVIASLSP
                     GYTACLWLGSVNMMLAVFNLIPGFPLDGGRVFRAIIWKLTGNQLRATRLASTTGRWFG
                     WAIMGIGLWNLLVLKSAGGLWLLLIGWFLSHLAAASYSQALAQQTLAPLRVRDVMRTR
                     FEFAPSGITIDEFINDYLLRSNQILWPLQGPAGVVGAVNFEQVASVPPDQRATRLVQE
                     VMVPVDRLPALDADDLATNALNTLLGAGDQPVPVVSGQRIVGLVRGSDILKWVMLHQG
                     GTPS"
BASE COUNT          986 a         1823 c         1657 g          922 t
ORIGIN      
        1 gcccactatt acccggcggg aacatacctc ccaggcgacc tggcggccgc agagcgcggc
       61 agcgactgac caccgagcct atggtttcgg ttcatcggca tgccacagat cgcgggtccc
      121 gatccgaagg aattccgccg gcagccgcga ggtaccctcg agcgcgtagt cggccagcag
      181 ctcgcccatc accggcgcga atttgaatcc gtggccgctg aacccgcaag ccacggtgac
      241 cgcgtcatga tcggggtgcc gatcgacgat gaaatgacca tcgcgggaat tcgtgtacat
      301 gcagatcgac gagccgatgc gcgagcctcg cagcccgggg aggagagacc tcacggcgcg
      361 cagcgtcgcc tgatactcgg cctcggaggg cggcgctttc gggccatcgg gttcgcatgt
      421 ctcgccggga gcatgatggg cgaacttggc tcccacacgc gcggtattcg ggacgcaggc
      481 agggaacccg tagtagatcc ccggatgccc cgcgatctcc agcgcccagc acggaaacct
      541 gcccagagag aattctgcgc agcgctcggg ccagatccag aaaagcgttt ggcgggtcac
      601 gttcagagcg acgcggggca gccgacagat gtcgctcgac cagcttcccg ccgcgatgac
      661 gagtgcaccg gcctcgagct ccccagcgct ggtgctgacc actaccccgc ctgattcggc
      721 ccgccaaccg tctacccggg tctggctcat cagcgtagcg ccgttggcaa tggcgagctt
      781 cgcaaacgcg ttgatgccgt gaccggcgag cacgaacccg gcttgcggct cgaaggccga
      841 gacgcagccg tcgggtaagc gaaaccccgg gaaccgatcg tgcagctcgg ctggcgcgag
      901 cggctcgaat tccagtcgat gggattccgc agccattcgt gagcccccga tcagcggccc
      961 atccggaggc cctacataga cggtgcccgt cggatagagc agttcgagcc cgctgagagc
     1021 ctcgagtcgg cgccagcctt cgtaagcccg ctcgagtagc ggaacatagt cgggatgctc
     1081 gaagtaagcc tttcggatga ggcgcgtatc acccccactg gagcccaagg agttgggcac
     1141 cgaccattgc tcgatgccca gcaccctggc gccccgggat gccagccgct ccaaagccgc
     1201 ggcgcccatg gcgccgacac cgaggacgat cacgtcgtag ctcatggcgc aatcctatct
     1261 cagctcggcg aagcgggcag agggaacagt gcacattgcg gctgccaatc ggctccatcg
     1321 ccggtatgat gctcgcaact gctccagccg tcgtgtcact tccgtggatc ctgtctccca
     1381 agccgccctc ggcgccgtgg tcgcccgcag tgtcagccat cgccaacttg gtgccggcgc
     1441 cgtcgcgctt ggggccgcgg caggcgccct gccggacatc gacgtgttct tctccctgga
     1501 tggtgactac ttcgaccagc tggttagcca tcgcgggatc acgcattcgc tgtttttcgc
     1561 ccccgtggtg ggccccctct tcgggtggct ggcctggcgc tggaccgccc gccgccgaaa
     1621 cgaccccggg gagccggcgg cattgcgcgc ctggatgctc gcgatgaccc tggcgatgct
     1681 ttcccacccg cttctagatt tactaacgcc gtacggcacg caactcctcc agcccttcag
     1741 cgacgcgaga ttcgcggtgg cggccatgcc catcatcgat ccgatctaca ccatcgtgtt
     1801 gctggctggg ctcgttgccg gatatcggtt cacggcggcc ggggggagga tcgcggcagc
     1861 aacgctgctc gtctcctgcg cttacctggg ttacggctgg cacctcaacg agggcgcgga
     1921 gcggtttgcc cgcgcccagc tcgcagccca ggggttgccg aacgtcgagg tagccgcgtt
     1981 cccgacgatc ctgcagattc accaccgacg tgtggtcgca cgcaccccgg acgccgaccg
     2041 ggtcgggttc ctctccatgt gggatccgtg cgatatcggc tggcaaaggg aacctcgcct
     2101 gccccgatca caggtggagg ccttcctccg cacgcgggaa ggggcgatat tcgactggtt
     2161 caccatgggt tggtcacact acgccatcca ggacgggcac atgacggcaa ccgatcttcg
     2221 ctacgggtct gatgagaacc cccgggcaag cgttttcacg gcatcggtcg ccctggacca
     2281 cctcgggcgc ccctcaggaa atatcgccgg cggccgcttc accccggacg ccaacggccg
     2341 gaacctcgaa cggctgatcg ccatgaccta cgcacccggc tgcggcacga gccctggacg
     2401 gatggcccaa acgagcctgg aagccggcgc cggctgatgg cgtcaacgga gcatctgcaa
     2461 cgatcgcttc gaacgcagtc agggccatgc cttccggacc catggatgac gacagtctga
     2521 ccatggagca ggaagccggc cggccgaacg acccaagcaa tctggacgtc gtggtggcca
     2581 tcgtcccgac gacggtcctc ctggcgctgt cggtcgccct cttcggcagt gactcatcga
     2641 gtggcgccaa tcagattgcc ctggtcatcg gagctgcgct ggccaccatg gtcggcgtga
     2701 agaacggcca ttcctggaag gtgatcgaag agaacatcgt tggcggcatt tccaccgcga
     2761 tgccggcaat catgattctc ctgtcggtcg gcagcctcat cggggtgtgg atgctgtcag
     2821 gaaccgtgcc cacgatgatc tattacgggt tgctgcttct cgaaccagcg gtgttttacg
     2881 ccgcgagttg tctcctgtgc gccgtgacgg cgctctccat cggaagctcc tggacggtag
     2941 cgggcacgct cggcatcggt ctgatcgggg tagccgccgg gctcgaactt tcgctgccga
     3001 tcaccgctgg ggccatcatc tccggagcct atttcggcga taagctctcc ccactttccg
     3061 ataccacgaa tctcgccccg gccgtgaccg agatcgacat cttcacccac atacggcaca
     3121 tgacctggac caccctgccg agcttcgtgt ttgcccttgg ggcgttcacc gccatcggcc
     3181 tgatggaaag cacgcccgag acgcaaacgt cactgcgcgt caccctggaa accctcgacg
     3241 aagcttttgc catcaacccc ctgatgctgc tccccgtcgc cctcgtcgtt cttctcgcgg
     3301 gcagaaagaa accggccgtt gcctgcatcc ttgccggcgt ggctctggga atcctgatgg
     3361 cgctcgtcta ccaacgtccc gccatcgtcc ggtttgcaga cgacgcgtcc ctggggccca
     3421 cggtcgccgc gctcaaggca atctggatcg tcctgttcga cggctacgcc ggctccaccg
     3481 gcgaagccaa tcttgacgag ttgctctctc gcggcgggat gagcagcatg ctcaacaccg
     3541 tctggctgat actggcagca atgaccttcg gagccgccat ggaacaggca ggggtgcttc
     3601 gccggctggt ccatgctctg gcatccgcag cgcgcagcac gggcaatctc atcgcgacga
     3661 cactggtcac ctgcatcggg gtgaacgtgc tggccgccga tcagtacatg tccatcgtct
     3721 tgaccggccg catgttcaga tcggaatatc gacgccgcgg gctgcacgag aaaaaccttg
     3781 cccggaccct cgaagacggc ggcaccatca cctcgcctct cgtcccatgg aacacctgcg
     3841 gcgcctacat ggcagcgacg ctgggcgtac ctaccctggc ctatctgccg ttctgtttct
     3901 tcaatctggc caacccgttc gtctcggccg cctacggcta cgcggggttc aagatcgagc
     3961 cgctaaaacc cggcggggaa ggcgaagctg ccccctcgcg cgctcaatga cgaagaccga
     4021 agacgggaac gtgatcacga cggagtgcca ccctggtgaa gcatcaccca tttcaggatg
     4081 tccgagccgc gcaccagacc gacgatccgc tgaccgctca ctaccggaac cggttgatct
     4141 ccggcaccga gcagcgtgtt cagggcattg gtcgccagat cgtcggcatc gagcgcaggc
     4201 agcctgtcca ccggcaccat cacctcctgg accagcctgg ttgcgcgctg atccggaggc
     4261 acgctggcaa cctgctcgaa gttgacggct ccaaccactc ccgcggggcc ctgaagcggc
     4321 cagagaatct ggttgctcct gagcaggtag tcgttgatga actcatcgat cgtgatgccc
     4381 gacggtgcga actcgaagcg agtgcgcatc acgtcgcgga cccgaagggg agcgagcgtc
     4441 tgctgagcga gggcctggga gtaactggcc gctgcgagat ggctcaaaaa ccagccgatg
     4501 agcagcagcc agagcccgcc agcggatttg agcaccagca ggttccacag gccgatgccc
     4561 atgatggccc agccgaacca gcgaccggtg gtacttgcca gccgtgttgc cctgagctgg
     4621 tttccggtca gcttccagat aatggcacga aacaccctgc cgccatcaag cgggaaaccg
     4681 ggaatcaggt tgaacacggc cagcatcatg ttcacggagc cgagccacag acaggcggtg
     4741 tatcccgggc tcaggctggc gatgacggtt tccggatctt ccgcaaacgc ttccctcgcc
     4801 agggtgtcca tgctcccaaa cgcaaccacc gtgaacaccg cgcccagcag agcgctcatc
     4861 aacggacccg caatggctat ggtcagctct gctgacgggg tttgcgcctc cgactcgatc
     4921 tcggctacgc cgccaaacag gaacagggta attctcggca cccttatccc gtatcggcgg
     4981 gccgtgagcg aatgagaaag ctcgtgggcc agcaacgatg cgaagaagag cacgccggcc
     5041 accagcgccg tcagccacgc cgtcgagggc ggccagtcgg gatgccactg gggaaacagc
     5101 ccgcctccca ggctgtacac gacgaggccg aagatgatgg caacgctgta atcgagccgg
     5161 atctcgatgc cgaagatgct gaacagcttc agccctccgc ccgcactcgg caacaatgga
     5221 ctcatggggc attctcccgt tcgttcagat aacgcccgat ggctgcctcg cccaagggat
     5281 cgggattctt gacagtcagg ctctgcgacc aatcccggcc gaagtcccgg tcgaaggatg
     5341 agtggacggc ggcaatcacc agggtggcgg ccagcgcgcc gccggttg
//
LOCUS       DBNHQR010000435         4447 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_168437, whole genome shotgun sequence.
ACCESSION   DBNHQR010000435 DBNHQR010000000
VERSION     DBNHQR010000435.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_168437"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..287
                     /locus_tag="AC16VR_09805"
     CDS             36..287
                     /locus_tag="AC16VR_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011493.1"
                     /translation="MDIDEVVSALRTERDELRVRLHLLKAEARDEFEELEKKWQHLES
                     RAAQVKEGAKESAEEIGAAAKTLAEEIGAAYTRIKKTLS"
     gene            361..1785
                     /locus_tag="AC16VR_09810"
     CDS             361..1785
                     /locus_tag="AC16VR_09810"
                     /inference="COORDINATES: protein motif:HMM:NF016032.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - modified amino acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HIR6011494.1"
                     /translation="MGDEIDRRYYDAEDFSDFRRRLDDETALLSDLFRRSEFSRRGDV
                     AGFELEVWLVDDEENPAPINDEYLKCLNNPLVVPELAEYNVELNGSPSALRGRVFSRL
                     HDELSATWRACRQAADRMGCALVTIGILPTVTEADLSSRYMSHMVRYQTLNDRVMALR
                     DGRPLELEIEGEGRLSTKHSDVMLEAAATSFQIHLQCRPDWAVRDFNASIVASAPIVA
                     ASANSPFLFGQSLWDETRIPLFEQAVDVGPNNPSRVSFGQGYIRESLLEIFEENRRDH
                     LILIPAVQDAPASKFSHVRFHNGTLWRWNRPLIGFDFDGQVHLRIEHRVVPAGPTIKD
                     CIANSAFYFGLIRGFGLQDEPVESRLPFEVARDNFYNCARYGLNGRVRWIRDGREEEL
                     LVRSLIMEDLLPLARRGLQSQDIPGEEIEAYLDVVAARVDSGQNGAAWQRRWVSLNGP
                     DLHGMVGAYRQQQDAGEPVHTWSL"
     gene            1789..2787
                     /locus_tag="AC16VR_09815"
     CDS             1789..2787
                     /locus_tag="AC16VR_09815"
                     /inference="COORDINATES: protein motif:HMM:NF048461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HIR6011495.1"
                     /translation="MPFNEFHSLPPGLLECEARELEGALGLPSLIHLPGGREPALFVS
                     VLLHGNEPSGWNGLRRLLADHPELPRSMSIFVGNVQAARAGMRALPGQQDFNRVWRGA
                     GGVEGEMARGVAGRLAERRFLAAVDLHNNTGHNPHYSVLSDLSVENLGMAYLFCDKAV
                     LIEEPDTVITHLFAGRCPAVTLELGPVSDPECEIRAYDYLLRCLRLDAVPAGHAEDFS
                     LYRSEARVHVPLGVSFHFADEGGAAPLVLTGGVEGVNFHELPAGFRFGSSEGRLEDVL
                     RVLDGAHQDVTERYFALQDGAILLKRPVVPAMYTTDPYVVDQDCLCYLMQRLEHAP"
     gene            complement(2829..4007)
                     /locus_tag="AC16VR_09820"
     CDS             complement(2829..4007)
                     /locus_tag="AC16VR_09820"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6011496.1"
                     /translation="MSELSEFRSDVAAWLNENCPPGARGRGQIPTGSSKVELEPDVKL
                     WLERCAERGWTAPTWPTEYGGGGHSAGETKIIYEEMAEIGARAPLMGMGLSMIGPTLL
                     EFGTPDQKERHVPSITRGEVHWCQGYSEPNAGSDLAALQTRAEDRGDHFVINGQKIWT
                     SGATLADWMFALVRTDFDAPKHEGISFVLLTMDQPGVTIKPIRLISGNSPFCETFFDD
                     AVASKADLVGELNKGWSVGKRLLQFERSGIGGLGGANARANPGSGLAEMARRFAGGAP
                     GRIQDPDVRQAVTRFNMNRAAFRLTTQRASEENRSGTPGAATSIFKLYGAALQQDAAE
                     LRRVFLGTQGLGADEGAFSDDELETTREWLATRATTIYGGTNEVQANIIAKRVLGLPD
                     "
     gene            complement(4039..>4447)
                     /locus_tag="AC16VR_09825"
     CDS             complement(4039..>4447)
                     /locus_tag="AC16VR_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011497.1"
                     /translation="IQSDPVAYHAALRDWVDKGKESDYALPAEEVVARSRTRNPEIAR
                     GHAHFELATQLEIDGQHDAAIRHFREAHRLVPDSWTFRRQAWSLEPVGEGPLARFWQG
                     PDPEDPQAWPYEGDWLTDIRATGAQNYNDPWRP"
BASE COUNT          713 a         1424 c         1462 g          848 t
ORIGIN      
        1 tagggcgcct gacaacaact ctattaggag tgatcatgga tattgatgaa gtggtttcag
       61 ccctgcgcac ggaacgggac gagttgaggg tgcggttgca cctgctcaag gccgaagctc
      121 gcgacgagtt cgaggagctg gagaagaagt ggcagcacct cgagagccgt gcggctcagg
      181 taaaggaagg cgcgaaggag tccgccgagg aaatcggcgc cgcagctaag acgcttgccg
      241 aggagatcgg tgccgcctac acgcgaatca aaaagacgct ttcctaggac atagacggac
      301 ctgccgcccc ggcccggcgg agcggttgcc gtcaggctga gctcgagacg cgggatttac
      361 atgggcgacg agatcgatag acgctactac gatgctgagg acttcagcga tttccgccgc
      421 cgactcgatg atgaaaccgc gctgttgagc gatctgtttc gtcgcagcga gttcagccgc
      481 cggggcgacg tggccgggtt cgagctcgaa gtctggctgg tggacgacga ggaaaatccc
      541 gcgcccatca acgacgagta tctcaagtgc ctgaacaacc cgctcgtcgt tcctgagctt
      601 gccgagtaca acgtcgagct caacggcagc ccgtccgcgc tccggggccg ggtcttctcc
      661 cggctgcacg acgagctgag cgctacctgg cgcgcctgca ggcaggccgc ggatcggatg
      721 ggctgcgctc tggtgaccat cggcatcctg cctacggtga ccgaggccga cctcagctcc
      781 cggtacatgt cgcacatggt ccgctatcag acactcaacg atcgggtcat ggcgctgcgc
      841 gacgggcgtc ccctcgagct ggaaatcgag ggtgaagggc ggctttccac gaaacactcg
      901 gacgtgatgc tcgaagcggc cgccacgtct ttccagattc acctgcagtg ccggccggac
      961 tgggccgtca gggacttcaa cgccagcatc gtggcttctg cgcccatcgt cgcggcaagc
     1021 gccaactcgc cgtttctttt cggtcagtct ctttgggacg agacccgcat cccgctcttc
     1081 gagcaggccg tcgacgtggg acccaacaac ccctcccggg tgagcttcgg tcagggttac
     1141 atccgcgagt ccctgctgga gatcttcgag gagaaccgcc gcgaccacct catcctgatc
     1201 cccgcggttc aggacgcccc ggccagcaag ttcagtcacg tgcgtttcca caacggcacc
     1261 ctgtggcggt ggaatcgccc gctcatcggc ttcgacttcg acggccaggt ccacctacgc
     1321 atcgagcacc gggtggtccc ggcaggaccg accatcaagg actgcatcgc caacagcgca
     1381 ttctatttcg gcctgattcg cggctttggc ctgcaggatg agccggtgga gtcgcggctg
     1441 ccgttcgagg ttgccagaga caacttctac aattgcgctc gctatgggct caacggcaga
     1501 gtgcgctgga ttcgggatgg acgtgaggag gagctgctgg ttcgaagcct gatcatggaa
     1561 gacctcctcc ccttggcgcg ccgtggcctg cagtcacagg acatcccggg ggaggagatc
     1621 gaagcctatc tggacgtggt ggcggctcgg gtcgactcgg ggcagaacgg agccgcctgg
     1681 cagcgtcgct gggtttccct caacggcccg gacctgcacg gcatggtcgg tgcttaccgc
     1741 cagcagcagg atgccggcga acctgtccac acctggtctc tctagtcggt gcctttcaac
     1801 gagttccact cgctgccgcc gggtctgctg gagtgtgaag cccgggagct ggaaggggcc
     1861 ctcggtttgc ccagcctgat tcatctgccc ggcgggcggg agccggcact gttcgtgtcc
     1921 gtgctgttgc acggcaacga gccgagcggc tggaacggtc tgcgtcggct tcttgccgac
     1981 caccccgaac tgccccggtc catgtcgatt ttcgtgggca acgtgcaagc cgcccgggcg
     2041 gggatgcggg cgttgccggg tcagcaggat ttcaatcgcg tctggcgggg ggcaggtggt
     2101 gttgagggtg agatggcgcg gggcgtggct ggccggttgg ccgagcgccg gtttctggcc
     2161 gccgtcgatc tgcacaacaa cactggccac aacccgcatt actctgttct ctccgatctg
     2221 tcggtggaga acctgggcat ggcctatctc ttctgcgaca aggccgtgct gatcgaggaa
     2281 ccggataccg tcattaccca cctttttgcc ggccggtgtc cggcggtcac gctggagctc
     2341 ggcccggtgt ccgatccgga gtgcgagatt cgcgcctacg attatctgct gcgctgcctg
     2401 cgcctggatg cggtgccggc cgggcacgcc gaggacttca gcctctatcg gagcgaagcc
     2461 cgcgttcacg tcccgttagg tgtgagcttt catttcgctg acgaaggcgg cgccgcgccg
     2521 ctggttctga ccggaggcgt ggagggggtc aatttccacg agcttcccgc gggatttcgg
     2581 tttggcagct ccgaaggccg cctagaggac gtgctgcgtg tcctggacgg cgctcaccag
     2641 gacgtgacgg agcgttattt cgctctgcaa gacggcgcca tcttgttgaa gcggccggtg
     2701 gtccctgcca tgtacacgac cgatccttac gtcgtggatc aggattgcct ttgctacctg
     2761 atgcaacgcc tagagcacgc gccgtgacgg gcttcagccg ccattgccgg caaacggcgg
     2821 gctgctcgtc agtccgggag ccccagcacg cgttttgcga tgatgttggc ctgcacctcg
     2881 ttggtcccgc cgtagatcgt cgttgccctg gtggccagcc attcgcgcgt cgtctccagc
     2941 tcgtcgtctg agaatgctcc ctcgtcggct cccagaccct gggtgccgag gaacacgcgg
     3001 cgcagctccg ccgcgtcctg ctgcagcgcg gccccgtaca gcttgaagat ggatgtagcc
     3061 gcgccgggcg tgccggatct gttctcctcc gaggcgcgct gggtggtgag ccggaacgcg
     3121 gcccggttca tgttgaaacg ggtgacagcc tggcgcacat ccgggtcctg gatcctgccg
     3181 ggcgcgccgc cggcgaaacg tcgagccatc tctgccaggc cgctgccggg gttggcccgg
     3241 gcattggcgc cgccaaggcc gccgatgccg gatcgctcga actggagcag ccttttgccc
     3301 accgaccagc ctttgttcag ttcgccgacc agatccgcct tgctggccac ggcgtcgtcg
     3361 aagaaggtct cgcagaatgg cgagttcccg gagatcaggc ggatgggctt tatggtcacg
     3421 ccgggctgat ccatggtcag gagaacgaag ctgatacctt cgtgcttagg ggcatcgaag
     3481 tcggtgcgca ccagggcaaa catccagtcg gccagcgttg ctccggaagt ccagatcttc
     3541 tgcccgttga tcacgaaatg atcccctcgg tcttctgccc gggtctgcag ggcggccagg
     3601 tcggagccgg cattcggctc agagtacccc tgacaccagt gcacctcgcc gcgcgtgatc
     3661 gatggaacgt gtctttcttt ctgatccggg gttccgaact ccagcagcgt cggcccgatc
     3721 atggacagcc ccatgcccat cagcggtgcc cgcgcgccga tctcggccat ttcctcgtag
     3781 atgatcttcg tctcgcccgc agagtggccc ccgccgccgt actcggtcgg ccaggtcggc
     3841 gccgtccagc cgcgctcggc gcagcgctcc agccacagct tcacgtccgg ttcgagctcc
     3901 accttgctgc tgcccgtggg gatctgcccg cgcccgcgag cgccgggtgg acagttttcg
     3961 ttcagccagg cagcgacgtc gctccgaaac tcgctcagtt ccgacatgtt gcttgctcca
     4021 gtgggagagg gtggggcgtc atggtcgcca cggatcgttg tagttctggg cacccgtcgc
     4081 tcggatgtcg gtaagccagt cgccctcgta gggccacgcc tggggatcct cgggatcggg
     4141 cccctgccag aatcttgcca gaggcccttc gcccacgggc tccagagacc aggcctggcg
     4201 tcgaaaggtc caggaatcgg gcaccagccg gtgggcctcc cggaagtgcc ttatcgcggc
     4261 gtcgtgctgg ccgtcgattt cgagttgggt ggccagctcg aagtgggcgt gcccgcgggc
     4321 aatctcggga ttgcgggtac gtgaccgcgc gaccacctcc tcggcaggca gcgcgtagtc
     4381 gctctccttg cctttgtcga cccagtcgcg cagcgcggca tggtacgcga ccggatcaga
     4441 ctgaatt
//
LOCUS       DBNHQR010000436         1586 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_41886, whole genome shotgun sequence.
ACCESSION   DBNHQR010000436 DBNHQR010000000
VERSION     DBNHQR010000436.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_41886"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1586
                     /locus_tag="AC16VR_09830"
     CDS             <1..>1586
                     /locus_tag="AC16VR_09830"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6011498.1"
                     /translation="AIADSDLLIFYASPSSIESKFCSQELQFALSRDIPVITVMLEET
                     PFPPWMELALGNHQILQRFDVPQRAYRSLLESGIANQLDQIPPAAPPLTSPRGRWRGM
                     WIGAGAVTALVFVLWRSFLPEPLAIAVAPFETDGSLRAAEMAGGVTAELSQGLTNLDL
                     MPVKSRLPDRRPAAGAFVGGSNDASHVIIGHVQLLDPGYRIRTSLIATEDDQEIWAQT
                     FVYVEDDPKEVQHQHVQNLLVALPDVLVGTIPALDRAAPSARAAAQQIYGEGARMLAQ
                     PGELQHVDTIRQLFEQALDNQPGFGLALLGLCDAHARTYQESRELDDLLHAETRCLQA
                     LAVGSEPLKAHLALGRVFLARGEAQLARFHFQRASAVAPGLAEPLIGLADVADQNGDL
                     EMARAYYEQALALPGTSWEAHLAYGNHLLYQGEFRAAAEQYEVVADLYPAAAIAYVNL
                     GAAWYSLGEFERASQAWRKSVELEPNPMSWGNLGAAQTYARNFSAAANAYREAAALAP
                     SDHRWLGHLGEILAYDPTGD"
BASE COUNT          282 a          523 c          501 g          280 t
ORIGIN      
        1 cgccatcgcg gacagtgatc tgctgatctt ttacgcctcc ccgtcctcca tcgagtccaa
       61 attctgctcc caggaattac agttcgctct gagccgcgac attcccgtca taacggtcat
      121 gctggaagaa accccgtttc ctccatggat ggaactggcg ctgggtaatc accagattct
      181 gcagcgcttc gacgtgccgc agcgtgcgta ccgatccctg ctggaatcag ggatcgcaaa
      241 tcagctcgat cagattccac ccgcggcccc gcctttgacg tccccgcgcg gtcgctggag
      301 gggaatgtgg atcggcgccg gagcagtcac cgcgctcgtc ttcgtcctgt ggcgaagctt
      361 cctgcccgag cctctggcta tcgccgtagc gcccttcgaa accgacggga gccttcgagc
      421 ggctgagatg gcggggggcg tcacggcgga actctcacaa ggactcacca acctggatct
      481 gatgccggtg aagagccggc tcccagatcg gcgccccgca gcaggcgcgt tcgtcggcgg
      541 cagcaacgac gcaagtcatg taatcatcgg acacgtccag ctcctggacc ccggttaccg
      601 gatccgcacg tctctcatcg caaccgagga tgaccaggag atctgggctc agacgttcgt
      661 gtacgtcgag gacgacccga aagaggttca acatcagcat gtgcagaatc tgctggtggc
      721 cttgccagac gtgcttgtcg gcaccattcc agccctggat cgggccgcgc cgagcgcgag
      781 agcggcggcg cagcagatct acggcgaggg tgctcggatg ctggctcagc ccggcgagct
      841 gcagcacgtg gatacgatcc gacaactgtt cgaacaggcc ctggacaacc agcctggctt
      901 tgggctcgcg ctgttgggct tgtgcgacgc tcacgcccga acctaccagg agagccgcga
      961 gctagacgac ctgcttcacg cggagacccg ctgcctgcag gcgctggccg tgggttccga
     1021 accgctgaag gcgcatctgg ccctaggccg ggtctttctg gcccgaggcg aggcacagct
     1081 cgcccgattc cactttcagc gtgccagcgc ggtggctccc ggccttgcgg aaccactgat
     1141 cggcttggcg gacgtggccg accagaacgg cgatctggag atggctcgcg cctactacga
     1201 acaggccctg gcgctcccgg gaacgagctg ggaagcccac ctcgcctacg gcaatcatct
     1261 gttgtaccag ggcgagtttc gggcggccgc cgagcagtac gaggtggtgg ccgatctgta
     1321 ccctgccgct gccatcgcct atgtcaatct gggcgcggcc tggtacagcc tgggcgagtt
     1381 cgagcgggcg agccaggcgt ggcgcaagag cgtcgagctc gagcccaacc ccatgagctg
     1441 gggaaacctg ggcgctgcac aaacttatgc gcgaaatttt tctgctgcgg cgaacgcgta
     1501 ccgggaggcc gccgccctgg caccctccga tcaccgctgg ctcggccatc tgggggaaat
     1561 cctcgcctac gacccaaccg gtgatg
//
LOCUS       DBNHQR010000437         5164 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_41922, whole genome shotgun sequence.
ACCESSION   DBNHQR010000437 DBNHQR010000000
VERSION     DBNHQR010000437.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5164)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_41922"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1236
                     /locus_tag="AC16VR_09835"
     CDS             <1..1236
                     /locus_tag="AC16VR_09835"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HIR6011499.1"
                     /translation="SNGYLGVLHAAVTDVSAWHGHYWNPAQPAPTRGEAPGALGVVEP
                     VWQPLPVKHVVLIVEESFSGEVWDDLALRGTYMPRFSSLADSGVLFENVYSTGTRTTR
                     GLEALLNGFPPLPGIAVNQRGGFERLPSLARALSLAGFHNVFVYGGWPGFSNFFPYWR
                     ALGYEELTSRDDFPEPRFETSWGVADEHLFEAIREHMSRLTQNHERVFLTSLTVSHHR
                     PFDFPAGRVPFPAEERRSEYALAYADWALGQFIEDASGEPWFDDTLFVIAADHGPRIS
                     GDAPIPVAGFRVPLLLYAPAHLNPRRVDHLGSLMSFGVTLLELLGLPNDEGLYGDNLL
                     LTEQGLVPMEHEYQVGLLTRDGLTVLHLGGEVSGWRQEDDRLVLDAPDWEVAARAARL
                     FHGAHQWFYGSASAAADGR"
     gene            complement(1211..4495)
                     /locus_tag="AC16VR_09840"
     CDS             complement(1211..4495)
                     /locus_tag="AC16VR_09840"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HIR6011500.1"
                     /translation="MVGIYHHNPLTVGTLWALALLWAAVPLLMARRANRSTLDEAARN
                     ALESSPCGSAVIDATGRIVWVNAAFAAQIDRGSDSMAGTLLSDVAREDLWAELESRRE
                     ELDRGVVIDVSSCLVRADGEEVWVAGHVRAMEPESGRLCLQLVDVSSALGAQDSLAKS
                     VAEFRQALESSNDLILMVSKHGKINYANPVATSCLAGDADLNEAVITDLVELNDRERF
                     ITALKNSRKKGEVAELSGLHLLRPDRDSDFPVAARIAGLRTPDGVGGAIVTCRLMDDQ
                     IASERELRSSEARFSRIFHSSPDAILIVRNADSTILDFNAGFIRLLGYTREDAIGQAE
                     SDMQLWVHEAERERILNNLQTDQEVTAQETKLRRKNGAVVYVELSLRYIEIEGELCIL
                     WIGRDVSERAIAEIARRQSEEKFVEVFNRSPDGIAIVRQEDMLICDINEAFVNSSGHQ
                     REDLIGESLFELDVVSESALADAALRLEEDDHFSNLEMTLHTKSGEEIPALVSATTLE
                     VEEEPCFLIIAKDLRDLRATEEQLRASEQRFRGAFENAPIGILLVDMDGKVFQANRFA
                     AELLSYRNASMRNLHISRLVPDDDRDKYNDLMQRLARGHQATLRTEQRMLCADGAEIW
                     TNMHVVLLRSQDGEPAYGIVQIADITEMKHSQQRMERMAFYDILTDLANRRLFYDRLS
                     QAIEYTTRNNRLAALLYLDLDQFKRVNDTLGHEAGDTLLRTVAERLSHNVRQEDTVAR
                     PGGDEFTVLLYNIGKPEDAGHVAEKILNELRRPITVAGEQLVMTTSIGISIIPSDGTD
                     PKQLMSNADLAMYRAKEQGRNQFQFYSKEMNTNAAHRLKTENELRAALERQEFELYYQ
                     PKVRLSDLQIVGVECLLRWHHPERGLLLPGEFIEVAEETGTIVDLGKWVIETACSAAT
                     QLAHEAGEPICTAVNISPRQFRDVELVSTVQRCLAQTGLDPDNLEIEITETMLMDDVE
                     AASATVCDLHDLGVRLAIDDFGTGYSSLNYLKKFPIDTIKVDRSFVADIPESQDDMAI
                     TAAVIAMARRLHLAVVAEGVQTREQLEFLQQHECEFAQGFLFSQAVPLEALKQLLVPN
                     VRLLRR"
     gene            4637..5050
                     /locus_tag="AC16VR_09845"
     CDS             4637..5050
                     /locus_tag="AC16VR_09845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HIR6011501.1"
                     /translation="MNYTLSVSTGRQGLYDITHKVVECLGRSGISEGLVTVFVRHTSA
                     SLLIQENADPSAREDLESWLNRLVPEVDELYTHRAEGPDDMPAHIKSALTATSLSIPV
                     QDGAMLLGTWQGVFLWEHRHHHDRREIIVSVLDGS"
BASE COUNT          833 a         1624 c         1695 g         1012 t
ORIGIN      
        1 agcaatggtt acctgggcgt gctccacgca gccgtgaccg acgtttctgc ctggcacggt
       61 cactactgga acccggcgca gcccgccccg acacgtgggg aagcacctgg cgctctgggg
      121 gtggtggagc cggtctggca gccgctcccg gttaagcatg tagtgctgat cgtggaggaa
      181 tccttctccg gcgaggtttg ggacgatttg gcgcttcgtg ggacctacat gccacgtttc
      241 tcttcactcg ccgattccgg cgtgcttttc gagaacgtct actcgacggg aacccgcacg
      301 acccgggggt tggaggccct gctgaacggc tttccgcccc tgccgggcat cgccgtcaat
      361 cagcgcggcg ggttcgaacg gctgccctcc ttggcgcggg cattgagcct tgccgggttt
      421 cacaacgtct tcgtttacgg cggatggccc ggattctcga actttttccc ctactggcgt
      481 gccctgggat acgaagagct gaccagccgc gacgacttcc cggagccgcg tttcgaaacc
      541 agctggggcg tggccgacga gcatctgttc gaagccatcc gtgagcacat gagccgtctt
      601 acccagaacc acgagcgggt gttcctgacg tcgctgacgg tcagccatca tcgcccgttc
      661 gactttccgg cagggcgggt cccgtttccg gccgaggaac gccgctcgga gtacgcgctg
      721 gcctatgcgg actgggccct gggccagttc attgaagatg cgagcgggga accctggttc
      781 gacgacaccc tgttcgtcat cgctgccgac cacggccccc gcatcagcgg cgacgcgccc
      841 attccggtcg cgggttttcg cgttccgctg ctgctgtacg cgccagctca cctgaacccg
      901 cgtcgggtcg accatctggg gtcgctgatg agtttcggcg tcacgctgct cgagctgctg
      961 gggcttccga acgacgaagg cctctacgga gacaatcttc tgctcaccga acagggcctc
     1021 gtgcccatgg agcatgaata ccaggtgggt ctgctcaccc gggatggcct gaccgtgttg
     1081 caccttggcg gtgaggtctc ggggtggcgg caggaagacg accggctcgt gctcgatgcg
     1141 cctgattggg aagtggcggc acgggcggcg cgcctcttcc acggcgccca tcagtggttc
     1201 tacggtagcg ctagcgccgc agcagacgga cgttaggcac cagcagctgt ttcaaagctt
     1261 ccagcgggac ggcctggctg aaaagaaacc cctgggcgaa ttcgcactcg tgctgctgca
     1321 ggaactcgag ctgctcgcgg gtctgcacgc cttcggcgac cacggccagg tgcagccgtc
     1381 gcgccatggc gatcacggcc gcggtgatgg ccatgtcatc ctggctctcg gggatgtcgg
     1441 ccacgaagct gcgatcgacc ttgatggtgt cgatgggaaa tttcttcagg tagttcaggg
     1501 aagaataccc ggtaccgaaa tcgtcgatgg ccagccgcac gcccaggtcg tggaggtcgc
     1561 acaccgtggc cgatgccgcc tccacgtcgt ccatcagcat ggtttccgtg atctcgatct
     1621 ccagattgtc ggggtccagg cctgtctgcg ccaggcagcg ctgcacggtg ctcaccagct
     1681 cgacgtcccg gaactggcgc ggcgagatgt tcaccgccgt gcagatcggc tcgcctgcct
     1741 catgagccag ctgggtggcg gcggagcagg ccgtctcgat gacccacttg ccgagatcga
     1801 cgatcgtgcc ggtttcctcg gcgacctcga tgaactctcc cggcagcagc aggccgcgct
     1861 cggggtgatg ccagcgcagc aggcactcca cgcccacgat ctgcagatcg gacaggcgca
     1921 ccttgggctg gtagtagagc tcgaactcct gccgctcgag agcagcgcgc agctcgttct
     1981 cggtcttcag acggtgggcg gcgttggtgt tcatctcctt gctgtagaac tggaactgat
     2041 tgcgcccctg ttcctttgcc cggtacatcg cgaggtcggc gttggacatg agctgcttgg
     2101 gatccgtgcc gtcgctcggg atgatcgata tgccgatgct ggtggtcatc accagctgct
     2161 ccccggccac cgtgatcggt cgtctcagct cgttgagaat cttctcggcg acgtgccccg
     2221 cgtcttccgg cttgccgatg ttgtagagca gaacggtgaa ctcatcgccc ccgggcctgg
     2281 cgaccgtgtc ctcctggcgc acgttgtggc tcagccgctc ggccaccgtt cgcaggaggg
     2341 tgtctcccgc ctcgtggccg agggtgtcgt tcacccgctt gaactggtcg aggtccaggt
     2401 acagcagagc ggcaaggcgg ttgttgcgcg ttgtgtactc gatggcctgg gagagccggt
     2461 catagaacag acgccggttg gcgaggtcgg tgaggatgtc gtagaaggcc atgcgctcca
     2521 tgcgctgctg gctgtgcttc atctccgtta tgtcggcaat ctgcacgatg ccgtaagccg
     2581 gctccccgtc ctgagatcgc aggagcacca cgtgcatgtt ggtccagatc tctgcgccgt
     2641 ccgcacaaag catgcgctgc tcggtgcgca gcgtggcctg atgccctctg gcgagccgct
     2701 gcatcaggtc gttgtacttg tcgcgatcat catcgggaac cagacgcgag atgtgcaggt
     2761 tcctcatgga ggcgttgcga tacgacagga gctcagccgc gaaccggttc gcctgaaaga
     2821 ccttgccgtc catgtcgacc agcagaatgc cgatgggcgc gttctcgaaa gcaccgcgga
     2881 agcgctgctc actggccctc agctgttcct cggtagcgcg cagatcgcga aggtccttgg
     2941 caatgatgag gaagcagggt tcctcttcga cctcgagcgt ggtggccgac accagggcgg
     3001 gaatctcctc gccggacttg gtgtgaaggg tcatctccag gttggagaag tggtcgtcct
     3061 cctccaggcg cagggcggca tctgcgaggg ccgactcgct caccacgtcc agctcgaaaa
     3121 gggactcccc gatcagatcc tcgcgctggt ggcctgagga attcacgaac gcttcgttga
     3181 tgtcgcagat cagcatgtct tcctggcgga cgatggcgat gccgtccggg ctgcggttga
     3241 agacctcgac gaatttctcc tcgctctgcc gtcgggcgat ctcggcgatg gcccgctcgg
     3301 acacgtcccg accgatccac aggatgcaca gctccccctc gatctcgatg tagcgcaggg
     3361 acagctccac gtacaccacc gctccgttct tgcggcgcag cttcgtctcc tgggcggtta
     3421 cttcctggtc ggtctggagg ttgttgagaa tgcgttcccg ctccgcctcg tgcacccaca
     3481 gctgcatgtc cgattccgcc tggccgatgg cgtcttcgcg cgtgtagccg agcaggcgga
     3541 tgaagcccgc gttgaaatcg aggatggtgg agtccgcgtt gcggacgatg aggatggcgt
     3601 ccgggctcga gtgaaagatt cgggagaacc gggcttcgga gctgcgcagc tcgcgctccg
     3661 atgcgatctg gtcgtccatc agccggcagg tcacgatggc tccgccaacg ccgtcgggcg
     3721 tgcgcaggcc ggcgatccgc gccgccaccg ggaagtcgct gtcgcggtcg gggcgcagca
     3781 ggtgaagacc ggagagctct gccacctcgc ccttcttgcg ggagttcttc agggcggtga
     3841 tgaatcgctc gcggtcgttc agctcgacga ggtcggtgat cacggcctcg ttgaggtccg
     3901 cgtccccggc aaggcaggaa gtggccaccg ggttcgcgta gttaatcttg ccgtgcttgg
     3961 agaccatcag gatgaggtcg ttgctcgatt ccagggcctg acggaactcg gccacggact
     4021 tcgccaggga gtcctgggcg ccgagggcgg aagagacgtc caccagttgc aggcagagcc
     4081 ggccgctttc gggctccatg gcccggacgt ggccggcgac ccagacctcc tccccgtcgg
     4141 cgcggacaag gcaggaggag acgtcgatca cgacgccgcg gtcgagctcc tcgcggcggc
     4201 tttccagctc ggcccagagg tcttcccggg caacgtcgct caggagggtg cctgccatgg
     4261 agtcgctgcc tcggtcgatc tgggcggcga acgccgcgtt tacccagacg atacggcccg
     4321 ttgcgtcgat gaccgcgctg ccgcagggcg aggactccag ggcgttgcga gctgcctcgt
     4381 ccagcgtcga tcggtttgcg cggcgcgcca tgagcagggg cacggcggcc catagcaggg
     4441 cgagcgccca gagcgtgccg acggtcagcg gattgtgatg gtaaataccc accacaagcc
     4501 ctgccgagat gcatacgagt ggtcctaggg cgaccagaag ctgtcgtaca cgtttgccct
     4561 gcatgtcttt gttgttcccg gatcccggcg atcgctatgc ttagcggctg tccgcgcgtg
     4621 gtctgagatt tcttccatga attacacgct gagcgtatcc accggtcgtc aaggcctcta
     4681 tgacatcacg cacaaagttg tcgaatgtct aggcaggtcc ggcataagtg agggcttggt
     4741 cactgtgttc gtccgccaca cctccgcgag cctgctgatt caggaaaatg cggacccgag
     4801 cgcccgcgag gatctggaat cgtggctgaa ccggctcgta ccggaggtgg acgagttgta
     4861 tacccatcgg gcagagggac cggacgacat gcccgcccac atcaaatcgg cactcaccgc
     4921 tactagcttg tcaattccgg tccaggatgg tgccatgctg ttggggacct ggcagggcgt
     4981 gtttctctgg gagcaccggc accaccatga ccggcgcgag atcatcgtca gcgttctaga
     5041 cggttcctga cgttctccat ccagcggcgg tgaccgtccg gatcgagaat catggacgag
     5101 aagtacagca gcagctcctt gtggcgctgg gtcagcttgg ccgcgcccat ggcgcgctct
     5161 ccgg
//
LOCUS       DBNHQR010000438         8918 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1687813, whole genome shotgun sequence.
ACCESSION   DBNHQR010000438 DBNHQR010000000
VERSION     DBNHQR010000438.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8918)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8918)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8918
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1687813"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="AC16VR_09850"
     CDS             <1..316
                     /locus_tag="AC16VR_09850"
                     /inference="COORDINATES: protein motif:HMM:NF015100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HIR6011502.1"
                     /translation="TILATIAVLAAAFWDGGSSTSSGSPLFHWFTGSTMLTVFFFATD
                     PVTHPVSPRGQIVFGIVVGTATFLIRAFGNYPDGAAFAILLGNALTPYLDKRLQEASA
                     NA"
     gene            321..797
                     /locus_tag="AC16VR_09855"
     CDS             321..797
                     /locus_tag="AC16VR_09855"
                     /inference="COORDINATES: protein motif:HMM:NF016121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HIR6011503.1"
                     /translation="MLIAAACAMLLALTDAWTADRIARNARSHELSQITDLAGAAPPA
                     DTTWNEDVWDFCSGTKLARSSAAGYGGPIRLVVGVSQGRILGIRVTAHQETPGLADFL
                     REPKRGWLAALNGRTGPETAAVDAVAGATITSEAVVRAVCDAMDRAQDASAEGCRA"
     gene            794..1459
                     /gene="rsxE"
                     /locus_tag="AC16VR_09860"
     CDS             794..1459
                     /gene="rsxE"
                     /locus_tag="AC16VR_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015476183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HIR6011504.1"
                     /translation="MSVAKPFRNAAIASNPAWAQLLGLCPLLAVSTSLVNALALGAAS
                     AFVLLGSNITIAAIRRWIPELARLPSFVLVIATFTTCAMLLMQAYAFELYLRIALFVQ
                     IIVTNCMILGRVEAFASRQPVGRAFLDAAGTACGFALALLAMGAARELIGHGSLMAGM
                     EQLIPGSESWRLNLSESGRGLLLASLPPGAFIIAGLLLGAGNAILRRPNPAPSGSAGE
                     NSA"
     gene            1456..2097
                     /gene="nth"
                     /locus_tag="AC16VR_09865"
     CDS             1456..2097
                     /gene="nth"
                     /locus_tag="AC16VR_09865"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014421910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HIR6011505.1"
                     /translation="MNNDKRRAIFERLRAANPAPTTELDYRNPFELLIAVILSAQATD
                     VSVNKATGPLFEAAGTPTEMKALGLRKLKSYIKTIGLFNTKAANILKTCEMLLTQHGG
                     EVPEDRQALEALPGVGRKTANVVLNTAFGHPTIAVDTHIFRVCNRTRIAPGKNVNQVE
                     QKLLKVVPDEFKRDAHHWLILHGRYTCIARKPRCGSCVIEDLCEFRQKTDLEG"
     gene            complement(2130..2696)
                     /locus_tag="AC16VR_09870"
     CDS             complement(2130..2696)
                     /locus_tag="AC16VR_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011506.1"
                     /translation="MNNYQVLFSGEVSPQANRDIVRAKLACELDIDDRKAAQLFSGRT
                     VVIKSQLSHRQAMELQERLASLGAICRVKDLTPKTRNNVADFRVDRSGKSDATLRDIT
                     AAHTECPRCGHMQLEAAHCTRCGVDIAAAVRQRQKEDLIIEKKIRELRAKQDGLLGRA
                     GAAPGKALQGAASSTVKTRGIGWFRKSS"
     gene            complement(2700..3911)
                     /locus_tag="AC16VR_09875"
     CDS             complement(2700..3911)
                     /locus_tag="AC16VR_09875"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HIR6011507.1"
                     /translation="MKIVLASSGGLDTSVICRWLQETYGAEVVTFTADIGQGEEVEPA
                     RAKAQAMGVQEIFIEDLRDEFVRDYVYPMFRANTLYEGEYLLGTSIARPLIAKRLVEI
                     AAETGAEAVAHGATGKGNDQVRFELGAYALNPDIRVIAPWREWDLNSREALMAFCEQH
                     QIPVDYQRGAKSPYSMDANLLHISYEGDNLEDPWQEADADMWRWTVAPEEAPDEAQLV
                     EIDFLQGDPVAVDGEKLGPADLLARLNSLGGAHGIGRNDIVENRFVGMKSRGCYETPG
                     GTLLLKAHRAIESLTLDREVTHLKDELMPRYATLIYNGYWWSPERKVLQALIDESQKP
                     VSGRVRLKLYKGAVSVVGRMSQEESLYDANVVTFEDDGGAYHQADAAGFIRLNALRLR
                     LGARRGRKLSE"
     gene            4080..4667
                     /gene="rnt"
                     /locus_tag="AC16VR_09880"
     CDS             4080..4667
                     /gene="rnt"
                     /locus_tag="AC16VR_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HIR6011508.1"
                     /translation="MADRFRGFLPVVVDLETGGFDPGSHAILEIAAVQLRFDRGRIAP
                     DRKWHQAVEPFPGSRVEAASLKVTGIDLKDPNRQAAPENDALRGLFREVRRAVREAGC
                     TRAILVAHNAAFDQQFLNNALARQQIKRSPFHPFSFIDTASLAAVTYGHTVLSEACAR
                     AHIEFEPARAHNALYDAERTAELFCAIVNAWGRPR"
     gene            complement(4675..5001)
                     /gene="grxD"
                     /locus_tag="AC16VR_09885"
     CDS             complement(4675..5001)
                     /gene="grxD"
                     /locus_tag="AC16VR_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017640308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HIR6011509.1"
                     /translation="MSEEVIATIEEQIATHPILLYMKGSPGAPQCGFSAQTVQCLVAC
                     GERFAYVDVLDNPEIRAALPGYANWPTFPQLWVRGELVGGCDIITEMFQAGELEPLIK
                     EAAETE"
     gene            5128..6306
                     /locus_tag="AC16VR_09890"
     CDS             5128..6306
                     /locus_tag="AC16VR_09890"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HIR6011510.1"
                     /translation="MPTETGTSEFDVEIDRTNTSSSKWDKYAGRDVLPFWVADMDFAM
                     PSFIRAAIAERLAHPVLGYTRTPDAAVQAFQNWLERCYGWEVPEEWLVWIPGVVTGFN
                     LAARAVAEPGGSVLIPTPVYYPFLSTPRHAGQDSIEVPLVRDGERWAMDFDAMSAACR
                     NGTRLFMLSNPQNPTGRAYTEEELSELAAFCLRHDLYLCSDEIHASIILAPEARHIPI
                     AAIAPEIAERTISLYAATKTYNIPGLGCAVAVIADARLRRDFRRAMGGLVPGIGPLAY
                     AASTAAFADASDWTTRLIDYLRGNHQRVREVVGERMTPVEATYLAWIDLTDLGFADPA
                     ARLEAFGIGLSEGADFGGAGYVRFNFACPRSLLDRGLARFRDALARLDAAQRAGASPD
                     "
     gene            6416..7576
                     /locus_tag="AC16VR_09895"
     CDS             6416..7576
                     /locus_tag="AC16VR_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine/succinylornithine family
                     transaminase"
                     /protein_id="HIR6011511.1"
                     /translation="MTHLMNNYARLPVAFARGSGAYLEDTSGKQYLDALSGIAVCGLG
                     HAHPRVTRAIAEQAETLLHTSNLYEVPLQERLADRLCEISGMDRVFFGNSGAEANEAA
                     IKIARLYGHKRGEKRPTIVVVDNSFHGRTMATLTATGNRKVQAGFEPLLAGFVRAPFD
                     DLDSVREIAGNNGDVVAVMVEPILGEGGIILPNDDYLPGLREICDQHEWLLILDEVQT
                     GNGRTGRYFAYQHADVLPDVVTTAKGLGNGVPIGACLARGQAAELLVPGTHGSTFGGN
                     PLVCAAAHAVLDALTEDGLIERAASLGERIAAGLKQSLAGNNRVVDIRGRGLMIAVQL
                     NEPCGRLVKAGLEAGILINVTRDDTVRLLPPLTLSDAEADQLVELVSGLVSA"
     gene            7595..8515
                     /gene="argF"
                     /locus_tag="AC16VR_09900"
     CDS             7595..8515
                     /gene="argF"
                     /locus_tag="AC16VR_09900"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIR6011512.1"
                     /translation="MAKRDFLTLLDCSGDELSALVARGRELKQNLARRDRPLLGHTAV
                     LILALNSTRTRVGFETGMNQLGGHTVVLNPEDTQLGRGEPIEDMARVLSEMVDVIIIR
                     TLEQTDIERFARAASVPVINAMSAKYHPCQLLADVQTFEELRGPIAGRTVAFVGDGHN
                     MCHSYINAARQWGFNLKIACPEGYSPDPSVLGSTNRASLVESAREAVEGADLVVTDVW
                     SSMGQEEEAARRREQFAPFQVNETLLDHAPDHTVFMHCLPAHRGEEISDKLLDDPRSA
                     VWQEAGNRLHSQKALLEFLILGEFPGSSGG"
     gene            complement(8542..>8918)
                     /locus_tag="AC16VR_09905"
     CDS             complement(8542..>8918)
                     /locus_tag="AC16VR_09905"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR6011513.1"
                     /translation="SKICFEITESVAVANIENAVEFMNRMKIIGCRFALEDFGSGRAS
                     YAYLRRLPVDYVKIDDVFVREIAANPGDYAVVRSINEIGHYMGKKTIAEFVEDEEVRA
                     RLAEIGVDYLQGMDAQSAIPLR"
BASE COUNT         1519 a         3029 c         2807 g         1563 t
ORIGIN      
        1 gaccattctg gccaccatcg ccgtgctggc ggccgcgttc tgggacggcg gcagctcgac
       61 cagcagcggg tccccgctct tccactggtt caccggctcc acgatgctga ccgtcttctt
      121 cttcgcaacg gaccccgtta cgcacccggt ttcgccgcgc ggtcagatcg tcttcggcat
      181 cgtggtgggc accgcgacct tcttgattcg ggccttcggt aactatccgg acggcgcggc
      241 attcgccatc ctcctcggca acgccctcac gccctatctc gacaagcgtc tgcaggaggc
      301 gtcggcaaat gcataagctc gtgctcatcg cggccgcctg cgcgatgctg ctcgccctca
      361 ccgatgcttg gacggcggat cgcatcgccc ggaacgcccg cagccacgag ctgagccaaa
      421 tcaccgacct ggcgggagca gcgccgcctg ccgatacgac ctggaatgag gacgtctggg
      481 atttctgcag cggcacgaaa ctcgcccgca gctcggcggc gggttacggc ggcccgatcc
      541 ggttggtcgt cggcgtgtcg caagggcgca tcctgggcat cagagtcacc gcgcaccagg
      601 aaacgcccgg ccttgccgac ttcctgcgcg agcccaagcg cggttggctt gccgcattga
      661 atggccggac cggaccggaa accgcggccg tcgatgcggt agcgggggcc acgatcacca
      721 gcgaagcggt cgtccgcgcg gtttgcgacg ccatggaccg ggcgcaggac gccagcgccg
      781 agggatgccg cgcatgagcg tcgccaagcc gttccgcaac gccgccatcg ccagcaaccc
      841 ggcctgggcg cagctgctgg gcctctgccc cctgctcgct gtcagcacca gcctggtcaa
      901 cgccctggcg ctgggcgcgg caagcgcgtt cgtgcttctc ggcagcaaca tcaccatcgc
      961 cgccatccgc cgatggatac cggagctcgc ccgtcttccg agtttcgtgc tcgtcatcgc
     1021 cacgttcacg acgtgcgcga tgctcctgat gcaggcctac gccttcgagc tgtatctgcg
     1081 aatcgcgctg ttcgtccaga taatcgtcac caactgcatg atcctcggcc gggtcgaggc
     1141 gttcgcgagc aggcagccgg tggggcgcgc tttcttggac gcggcgggca cggcttgcgg
     1201 gttcgcgctg gccctgctcg ccatgggcgc cgcccgagag ctcatcggcc acggctccct
     1261 gatggcggga atggagcagc tgattcccgg ctcggagtca tggcggttga acctgtcgga
     1321 gagcggacgc ggtctgctcc tggcgagcct gccgcccggc gctttcatca tcgccggcct
     1381 gctcctcggt gcgggcaacg cgattctgcg ccgaccgaac ccagccccat ccggctccgc
     1441 aggagaaaac tcagcgtgaa caacgacaaa cgccgcgcca tcttcgagcg cctacgggcg
     1501 gccaacccgg cgccgacgac agagctcgac taccgcaacc ccttcgagct gctgatcgcc
     1561 gtcatcctct ccgcccaggc caccgacgtc agcgtcaaca aagccacggg cccgctgttc
     1621 gaggcggccg gaacgccgac cgagatgaag gcgctcgggc tgcgaaagct caagagctac
     1681 atcaagacca tcggcttgtt caataccaag gcggccaaca tcttgaagac ctgcgagatg
     1741 ctgctcacgc agcacggcgg cgaggtcccg gaggatcgcc aagccctgga agcgctcccc
     1801 ggggtgggac gcaaaaccgc caacgtggtt ctcaacacgg cgttcgggca ccccaccatc
     1861 gccgtggaca cccacatctt cagggtctgc aaccgcaccc ggatcgcgcc gggcaagaac
     1921 gtcaatcagg tggagcagaa gctgctcaag gtcgtgccgg acgagttcaa gcgcgacgcg
     1981 caccactggc tgatactgca cggccgctat acctgcatcg cgcgcaagcc gcgctgcgga
     2041 tcatgcgtga tcgaggatct ctgcgagttt cgtcaaaaga ccgatctcga gggctgaggt
     2101 atccgcggcg cggccgcgca ggcgggcact caggacgact ttctgaacca gccgatgccg
     2161 cgagttttca ccgtcgacga agccgcgccc tgcagcgcct ttccgggcgc ggcgcccgct
     2221 cgcccgagca gaccgtcctg cttggcgcgc agctcgcgta tcttcttctc gatgatcagg
     2281 tcctctttct gccgctgccg gacggctgcc gcgatgtcga ccccgcagcg cgtgcagtga
     2341 gcggcttcca gctgcatgtg cccgcagcgc gggcactccg tgtgagcagc ggtgatgtcg
     2401 cgcagggtcg cgtcggattt accggacctg tccacccgga agtccgccac gttgttgcgg
     2461 gtcttcggcg tgaggtcttt gacccggcat atggcgccca ggctggctag acgctcctgc
     2521 agctccatgg cctgccgatg gctgagttga ctcttgatga ccaccgtccg gccgctgaaa
     2581 agctgcgcgg ctttgcggtc gtcgatgtcg agttcgcagg ccagctttgc ccgcacgatg
     2641 tccctattcg cctgcgggga aacctcaccg ctgaacagca cttgatagtt gttcatcgat
     2701 tactccgaaa gctttcgtcc tctccttgcg cccaagcgca ggcggagggc gttaaggcgg
     2761 atgaaacccg ccgcgtcggc ctgatggtac gcccccccgt cgtcctcgaa agtgacgacg
     2821 ttcgcatcgt acaggctctc ctcctgcgac atccgtccca cgacggatac cgcacccttg
     2881 tacaacttga gcctaacccg acccgagacg ggtttctgtg attcgtctat cagcgcttgg
     2941 aggacttttc tctcaggaga ccaccagtag ccgttgtaga tgagcgtcgc gtaccggggc
     3001 atgagctcgt ccttgagatg ggtgacttcc cggtccagcg tgagggactc gatggcgcgg
     3061 tgggccttga gcagcagagt tccccccggt gtctcgtagc agcctcggga cttcatcccg
     3121 acgaagcggt tctccacgat gtcgttgcga ccgataccgt gggcgccccc cagggaattg
     3181 agcctggcga gcagatcggc cggtccgagc ttctcgccgt cgacggccac gggatcgccc
     3241 tgcaggaaat cgatctcgac gagctgcgcc tcgtccggcg cctcctcggg cgccaccgtc
     3301 cagcgccaca tgtcggcatc cgcctcctgc cagggatctt ctaggttgtc gccttcgtag
     3361 gagatgtgga gcaggttggc atccatggag taaggagact tggcgccgcg ctgataatcc
     3421 acggggatct ggtgctgctc gcagaacgcc atcaacgcct cccgggaatt gagatcccac
     3481 tcgcgccacg gcgcgatcac ccgaatgtcc ggattcagcg cgtatgctcc gagctcgaag
     3541 cggacctggt cgttgccctt gccggttgct ccgtgagcga cggcctccgc gccggtttca
     3601 gcggcgatct cgacgagacg cttggcaatc agaggccgcg caatgctcgt cccgagcagg
     3661 tactcgcctt cgtagagagt atttgcgcgg aacatcgggt agacgtagtc gcgcacgaac
     3721 tcgtcgcgca gatcctcgat gaagatctcc tgcacgccca tggcctgggc cttcgccctg
     3781 gcaggctcca cctcctcacc ctggccgatg tccgcggtga acgtcaccac ctcggcacca
     3841 taggtttcct gcaaccagcg gcagatcacg gaggtatcca ggccacctga ggatgccagc
     3901 acaatcttca tcgtcacgtt tcccccgagt gcggtcgccg agaaaaaagg gtcgcaatct
     3961 taagtaactt gagcagggcc cgccagccgc cgtcccgcgg gaaacccttc cggccgggtg
     4021 ccttcccgct tccggcgcgc aaagtcgcgt caaagccgtg ttaatgtgcc cgcatggaga
     4081 tggcggatcg ttttcggggt tttctgccgg tggtggtcga tctggagacg ggaggcttcg
     4141 atcccggctc ccacgccatt ctggagatcg cagccgtgca gctgcgtttc gatcgcgggc
     4201 ggatcgcgcc ggaccggaag tggcatcagg ccgtggagcc cttcccgggc agccgggtgg
     4261 aggccgcttc gttgaaggtg accggcatcg acctgaagga tcccaaccgg caggcggcac
     4321 cggagaacga tgcgttgagg ggtcttttcc gggaagtgcg ccgggccgtg cgagaagccg
     4381 gctgcacgcg tgccattctg gtcgcgcaca acgccgcgtt cgatcagcag tttctcaaca
     4441 atgcccttgc gcgccagcag atcaagcgca gccctttcca tccgttcagc ttcatcgaca
     4501 ccgcgtctct ggccgcggtg acttacggtc acacggtgct cagcgaagcc tgcgcgcgtg
     4561 cgcacatcga gttcgagccg gcccgggcac acaacgcgct ctacgacgcc gagcgaacgg
     4621 ccgagctttt ctgcgccatc gtgaacgcct gggggcgtcc gcgttgaacg atcgtcactc
     4681 cgtctcggcg gcttctttga tcagaggctc gagctcaccc gcctggaaca tctcggtgat
     4741 gatgtcgcag cctccgacga gctcgccgcg cacccagagc tggggaaagg tcggccagtt
     4801 cgcgtagccc ggcagcgcgg ctctgatttc cgggttgtcg aggacgtcca cgtaggcaaa
     4861 tcgctctccg caggcgacca ggcactgaac cgtctgggca gaaaatccgc actgcggtgc
     4921 gcccgggctg cccttcatgt aaagcagaat cggatgtgtc gcgatctgct cctctatggt
     4981 ggcgatcact tcttcgctca ttgctcgtca accctcgacg cgaattgcgc ttggcatgga
     5041 atgccgacat tctaccctcc gatcaggccg cgcaacaggg agccggagcg cagtccatca
     5101 aggcgcggat cggtgcacgg agggaacatg ccgaccgaaa cgggaacgag cgagttcgat
     5161 gtcgagatcg atcgcaccaa cacgtcgagc tccaaatggg acaagtacgc aggccgcgac
     5221 gtgctccctt tctgggtcgc ggacatggat ttcgccatgc cgtcattcat ccgcgcggcc
     5281 atcgcggagc gcctcgcgca tcccgtgctg ggatacacgc gcactcctga cgctgcagtg
     5341 caggcgttcc agaactggct cgagcgctgc tatggctggg aagtgcccga ggagtggctg
     5401 gtctggatac ccggggtggt gacaggattc aacctggccg cgcgcgcggt ggccgagccc
     5461 gggggctccg ttctcattcc cacacccgtc tactacccgt ttctgtcgac cccccgccat
     5521 gcaggccagg actccatcga ggtcccgctc gtgagagacg gcgagcgatg ggccatggac
     5581 ttcgacgcca tgagcgccgc ttgccgcaac gggacacggc tgttcatgct gagcaacccg
     5641 cagaacccga cgggccgcgc gtataccgag gaggagctgt ccgagctcgc ggcgttctgc
     5701 ctgcgccacg acctctacct atgctcggac gagattcacg caagcatcat tctggcgccc
     5761 gaggctcgtc acattcccat tgccgccatc gctccggaaa tcgccgagcg caccatcagc
     5821 ctttacgccg ccaccaagac ctacaacatt cccggcctcg gctgcgcggt ggcggtgatc
     5881 gccgatgcgc gcctgcgacg cgacttccgt cgggccatgg gggggctcgt cccgggtata
     5941 ggaccgctgg cctacgcggc ttcaaccgct gcctttgcag acgccagcga ttggacgacg
     6001 cgcctgatcg actacctgcg cggcaatcac cagcgcgtgc gcgaggtggt cggcgagcga
     6061 atgacgcccg tcgaggccac ctatctcgcc tggatcgatc tcaccgatct cggctttgcc
     6121 gatcccgccg ctcgcctgga agcattcggc attggtcttt ccgaaggggc ggacttcggc
     6181 ggggccgggt acgtgcgttt caacttcgcc tgccctcgat ccctgctcga ccgcggcctg
     6241 gcacgctttc gagatgcgct cgcccggttg gacgcagcgc agagggcggg tgcgtcgccc
     6301 gattgaactg accactcccc tgcagtacga ttcagcgttt ttccgggcag caacagctgc
     6361 ctggaatggt tttggggagg cgaaaatctt ccctacgacg atccgcaggc agcacatgac
     6421 gcacctcatg aacaactatg cccgcctacc cgtggcgttc gcccggggta gcggcgcgta
     6481 tctcgaggac acgtccggaa agcagtatct ggacgctctc agcggcatcg ccgtctgcgg
     6541 cctggggcac gcccatcccc gggtcacgcg cgccatcgcc gagcaggcgg aaacgcttct
     6601 ccacacttcc aacctctacg aggttcccct gcaggagcgg ctggccgatc ggctctgcga
     6661 gatctcaggc atggaccggg tcttcttcgg caattccggc gccgaggcaa acgaggcggc
     6721 catcaagatc gcccggctct acggccacaa gcggggagaa aagcgaccca ccatcgtggt
     6781 cgtggacaac agctttcacg gccgcaccat ggcaacgctg acggcgacgg gaaaccgcaa
     6841 ggtgcaggcg ggcttcgagc ccctgctggc cggattcgtc cgcgctcctt tcgacgacct
     6901 ggactcggtt cgcgagatcg cgggcaacaa cggcgacgtg gtcgccgtga tggtggagcc
     6961 cattctcggc gaggggggca tcatcctgcc gaacgacgac tatcttcccg ggctgcgaga
     7021 gatctgcgac cagcatgagt ggctgctcat cctggacgag gtccagacgg gcaacggccg
     7081 caccggccgc tactttgcct accagcacgc cgacgtgctc ccggacgtgg tgacgacggc
     7141 caagggtctc ggcaacggcg tgcccatcgg tgcctgcctg gcccgtggtc aggcggcgga
     7201 actgctggtc ccgggcaccc acggctccac gttcggcggc aacccgctgg tctgcgccgc
     7261 ggcccacgcg gtgctggacg cgctgacgga agacgggctg atcgagcgcg ccgccagcct
     7321 cggcgaaaga atcgcagccg gcctgaagca atccctggcc ggcaacaacc gggtcgtcga
     7381 catccggggt cgcggcctga tgatcgcggt gcagctcaac gagccatgcg gcaggctggt
     7441 gaaggcgggc ctggaggccg ggattctgat caacgtgacc cgagacgaca ccgtcaggct
     7501 gcttccgccg ctgactttga gcgacgccga ggccgaccag ctggtagagc tggtgagcgg
     7561 tctcgtctcc gcctgacccg cggaacctgc caccatggcc aagcgcgact tcctcaccct
     7621 tctcgactgt tccggcgacg agctgagcgc gctcgtggcg cgaggccggg aactcaagca
     7681 aaacctcgcc cgacgtgacc gaccgttact cgggcacacg gcggtgctca tcctcgccct
     7741 gaactcgacc cgcacccggg tggggttcga gaccggcatg aatcagctcg gcggccacac
     7801 cgtcgttctc aacccagagg acacccagct cggccgcggc gagcccatcg aggacatggc
     7861 tcgggtgctt tccgagatgg tggacgtgat catcattcga accctcgagc agacggacat
     7921 cgaacgcttc gcccgcgcgg ccagcgtccc ggtgatcaac gccatgagcg cgaagtatca
     7981 tccctgtcag ctgctcgccg atgttcagac cttcgaggag ctgcgcggtc ccatcgcggg
     8041 gcgcacggtg gcgttcgtgg gcgatggcca caacatgtgc cactcctaca tcaacgcggc
     8101 ccggcaatgg ggcttcaacc tgaagatagc ctgtcccgaa ggctactcac ccgacccctc
     8161 ggtgctcggc tccacaaacc gggcaagcct ggttgaatcc gcccgggagg ccgtggaggg
     8221 agccgacctg gtggtcaccg acgtctggtc ttcaatgggc caggaggagg aagccgcccg
     8281 gcgccgcgag cagttcgcgc cgttccaggt caacgagacg ctgctggacc atgctccgga
     8341 ccacaccgtc ttcatgcact gcctgcccgc ccatcggggc gaagagatca gcgacaagct
     8401 cttggacgat ccccgctccg cggtctggca ggaagcaggc aacagattgc acagccagaa
     8461 ggcgctgctc gagtttctga ttctggggga atttccaggc tcgtccggcg gttgatttca
     8521 agccggagga cggctgggcg attaccgcag cgggatcgcg ctctgggcat ccatgccctg
     8581 caggtagtcc accccgatct cggccagccg ggcgcggacc tcctcgtcct cgacgaactc
     8641 cgcgatggtc ttcttgccca tgtagtgacc gatctcgttg atcgagcgaa ccacggcgta
     8701 gtcgccggga ttggccgcga tctcccggac gaacacgtcg tcgatcttca cgtagtctac
     8761 gggcaggcgg cgcaggtagg cataggacgc gcggcccgac ccgaagtcct ccagggcgaa
     8821 acggcagccg atgatcttca tgcggttcat gaactccacc gcgttctcga tgttggcaac
     8881 cgccaccgac tcggtgatct cgaagcagat cttgctgg
//
LOCUS       DBNHQR010000439         5315 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_168562, whole genome shotgun sequence.
ACCESSION   DBNHQR010000439 DBNHQR010000000
VERSION     DBNHQR010000439.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5315)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_168562"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1683
                     /locus_tag="AC16VR_09910"
     CDS             <1..1683
                     /locus_tag="AC16VR_09910"
                     /inference="COORDINATES: protein motif:HMM:NF049598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HIR6011514.1"
                     /translation="DSTPDVSVQTLTNLTAQDVSDLRGSPDVTILSVEGTYAENEPAL
                     TVKVQVVNENGRLVHQGVPVENFIFDSGATSLQGPTPGFRIIGHREEMTIADAEARGI
                     DRELALNSVGEDTRSEFEAEARANVSETDLDYVAGVEDNLPVVIYEVYVHMDPSGDGT
                     NELHKVLLAGDGSQRLISYEAVPDHPFSMGSVYPLPHTPVGEGLVHGLKDEQDAATSM
                     MREMLDNLQLSNRPGKEVVEGMVNLDDLMATELGSLVRVRAPNMIKDMAVPFVAAQSL
                     PIMQFLDQQIERKTGISASAMGFSPDMLQSTTKSAVDAANRAGMGQTEYMARNIAETL
                     LKPVFRRSARLLAENMDRAFWVELAGEPVQIDPRGWTSNFDLEVNVGLGSGQPEEKAA
                     VLREVAALQKEILMGRGVDNGLVSVNQFVNTLSDILALSGLHNPARYFGPVTPEVVQG
                     MLAKEQQAKQAPQGGDPTQGLVEAEKIKTQGRMEEAKLKASLDTQKAALDAKLRAVDG
                     MKRDDLARDKMLQELVLSAIEIQAKYGAQVDIASLRAEQARNTPPTNGGTQQ"
     gene            1925..3037
                     /locus_tag="AC16VR_09915"
     CDS             1925..3037
                     /locus_tag="AC16VR_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011515.1"
                     /translation="MTADGMLSAESGPDDQRILDMVAPPPPKDVKPARVEPEPESADA
                     EQLADDTEDQISDDDPPEQSEQDTDADPSSEDEPPQELDLGPVNRWPAEDREVFLALP
                     PEAQEIWLKRVRQNEAVLTKKSQELAEQRRQAEEQQHDLTEGMGRTTQLIEHLVARGR
                     AVDNALQQVDQFVQSGLFPQEPPIEMLDTDPIGYQRQQALHQKAVATVRGLQQQRAAL
                     LLEEEQQRGARFQSHLHREAQKLVSKVPELADPDPAKRDAFKSGVTQMLITKFGASPD
                     EIGKISDHRLLALAADHYRVLQRLERGDRAIQKGRDNSQRTAQGTTPTIRPAPRKSSQ
                     AAAPAKKRELKLRDKMKTSGSVEDTAAWIGGSAHGR"
     gene            3027..4004
                     /locus_tag="AC16VR_09920"
     CDS             3027..4004
                     /locus_tag="AC16VR_09920"
                     /inference="COORDINATES: protein motif:HMM:NF036552.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SU10 major capsid protein"
                     /protein_id="HIR6011516.1"
                     /translation="MAVNANTVETYDVSTIREDLQEALVSISPEECPFMQNAGQRSVS
                     QSLFEWPVMELAATDSSNRVIEGDDDPAVDAGTIAGRAQNYCQISDKVVSVSHTDDAS
                     DGAGDDHHRFNKQVAIKLRELKRDMEVMLLGAVVAAPGSSGVARSTAGLPAWLKTNTS
                     RGAGGTDPTVSGAGDFDGYPNAAPGAGTGRALTETIFNDVIELIWNEGGEPGMVLCNG
                     ARKRKISTFTGNASRYKNAEDRRVVAAIDLYASDFGFDIQIVPDRFMAVATDVYLCTP
                     GMYRLAYLEKTKQKELAETGHSRKRLVWNEYGLQVDNEKAHGVIADITS"
     gene            4066..4362
                     /locus_tag="AC16VR_09925"
     CDS             4066..4362
                     /locus_tag="AC16VR_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011517.1"
                     /translation="MPETCWIVNRARSTAHVPVPHDDPEVHDPRRIASGDYEEVPGSV
                     DTWKARLKGHAHLEVRARAPREGEDILAAAVLPKPPKQRGSTNRRSREQIDGAA"
     gene            4349..4678
                     /locus_tag="AC16VR_09930"
     CDS             4349..4678
                     /locus_tag="AC16VR_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011518.1"
                     /translation="MGLLDPQNGSLRAPDGQVTDRWSVDPETGIVHREQAEDVESVID
                     HNAHLRDFLPKYHGHAAWRHCASIPSTIANLLSRECGAAPGSPEFAAYAKRQIVLRFP
                     KLLVKGW"
     gene            4685..>5315
                     /locus_tag="AC16VR_09935"
     CDS             4685..>5315
                     /locus_tag="AC16VR_09935"
                     /inference="COORDINATES: protein motif:HMM:NF049196.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adaptor protein"
                     /protein_id="HIR6011519.1"
                     /translation="MAFTTHAEFKTEIEEWLDRPDLSAKIDSFIDIAEATIRRRCKGL
                     EREVRAQATVTAGQRYVALPTDYRGMKNLSIRPEDGGGYLEQRTPDELDRLTERNALS
                     SSTSTPFWYALNGNELELSPVAGADIILEMTYIETLSPISPSGTNLVFDRHPDIYLYA
                     TLCAASYYTVEDRRVPLWKASLTEAVNEYNGDAEQRKYGAAPLRMRPALK"
BASE COUNT         1068 a         1754 c         1707 g          786 t
ORIGIN      
        1 gactcgacgc cggatgtcag cgtgcagacc ctcacgaact tgaccgcgca ggacgtgtcg
       61 gatttgcgcg gttcgccaga cgtgaccatc ctgagcgtcg agggcaccta tgccgagaac
      121 gagccggcgc tgaccgtcaa ggtgcaggtg gtcaacgaga acggccgcct ggtacatcag
      181 ggcgtgcccg tcgagaactt catcttcgac agcggcgcga cctcgctgca ggggccgacc
      241 cccggttttc gcatcatcgg gcaccgcgag gagatgacca tcgccgacgc cgaggcgcgc
      301 ggtatcgatc gcgagctggc gctcaattcc gtgggtgagg acacccggtc ggagttcgag
      361 gccgaggccc gcgccaacgt gtccgaaacc gacctggact atgtggccgg cgtcgaggac
      421 aacctgcccg tggtgattta cgaggtctat gtccacatgg acccctcggg cgacggcacc
      481 aacgagctgc acaaggtgct cctggccggc gacggctcgc agcgcctgat ctcctatgaa
      541 gcggttcccg accatccgtt ctcgatgggc agcgtgtatc cgctgccgca cacaccggtt
      601 ggcgagggcc tggtgcacgg cctcaaggat gagcaggacg ccgccacgtc gatgatgcga
      661 gaaatgctcg acaacctgca actctcgaac cgccccggca aggaagtggt cgagggcatg
      721 gtgaacctcg atgacctgat ggcgactgag ctggggagcc tggtgcgcgt ccgtgccccg
      781 aacatgatca aggacatggc cgtgcccttc gtggcggcgc agagcctgcc catcatgcag
      841 ttcctcgatc agcagatcga gcggaaaacg ggcatcagcg cgtcggcaat gggtttctcg
      901 cccgacatgc tgcagagcac gaccaagagc gccgtggacg ccgcgaaccg cgccggcatg
      961 ggccagaccg agtacatggc gcgcaacatc gccgagaccc tgctgaagcc tgtattccgt
     1021 cgatccgcgc gcctgctcgc cgagaacatg gaccgcgctt tctgggtcga gctggccggc
     1081 gagccggtgc agatcgaccc ccgcggctgg acgagtaact tcgacctgga ggtcaatgtc
     1141 ggcctgggca gcgggcagcc cgaggaaaag gctgcggtgc tgcgcgaggt cgcggcgctg
     1201 cagaaagaaa tcctcatggg tcgtggcgtg gacaatggcc tggtgtcggt gaatcagttc
     1261 gtgaacaccc tgagcgacat cctcgccctg agcggcctgc acaaccccgc gcggtacttc
     1321 ggcccggtga ccccggaagt ggtgcagggg atgcttgcca aggagcagca ggccaagcag
     1381 gcgccgcagg gcggtgaccc cacgcagggc ctggtcgaag ccgagaagat caagacgcag
     1441 ggccgcatgg aagaggcgaa attgaaggcc tcgctggata ctcagaaggc cgcgctggac
     1501 gcgaagctgc gcgccgtgga cggcatgaag cgggacgacc tggcgcgcga caagatgctc
     1561 caggagctgg ttctgtccgc gatcgagatc caggccaagt acggcgccca ggtcgatatc
     1621 gcgtccctgc gtgccgagca ggcgcgcaac actccgccca ctaatggagg cacccagcaa
     1681 tgagcgaccc gacccctgcc cagcatcgcc tcgcgacggc gatggtcaac gactccgcct
     1741 accctgaatt gctcaagggg ttgcaggacg gcgcacacct gctgttccag gcttcggcgc
     1801 cctccgacac caagggtctg caagaggcta gacttttact cacggccata agtatgctag
     1861 atggtgcact acgcggttgg gcgcgcccag ctccgatcac accgcagaag aggaagcagg
     1921 cacgatgacg gccgatggta tgttgtccgc agagagcggc cccgacgatc agcgcatcct
     1981 tgacatggtg gcgcccccgc cgcccaagga cgtgaagccc gcgcgcgtgg aacctgaacc
     2041 ggagagcgcc gacgccgagc agctcgccga tgacaccgaa gatcagatca gcgacgacga
     2101 tccccccgag cagtcggaac aggacacgga cgctgacccc tccagcgaag atgagccgcc
     2161 ccaggaactg gatctcggcc ctgtcaatcg ctggcccgcc gaggaccgcg aagtctttct
     2221 ggccctcccc cctgaagccc aggaaatctg gctgaagcgg gtgcgccaaa acgaagcggt
     2281 actcaccaag aagtcccagg aactcgccga gcagcgccgc caggctgaag agcagcagca
     2341 cgacttgacc gagggaatgg ggcgcaccac gcagctgatt gagcatctgg tggcgcgagg
     2401 acgcgccgtc gataatgctc tgcagcaggt cgatcaattc gtgcagtcgg gcctgttccc
     2461 gcaggaaccc ccgatcgaaa tgctcgacac ggaccccatc ggctaccagc gccagcaggc
     2521 gctgcaccag aaagcggtcg ccactgtgcg cggcctccag cagcagcgcg ccgcgttgtt
     2581 gctggaggaa gagcagcaga ggggcgcgcg tttccagagc catctgcatc gcgaagctca
     2641 gaagctcgtc tcgaaagttc ctgagctggc cgacccagac cctgcgaagc gagacgcatt
     2701 caaatctggt gtgacccaga tgctgatcac caaattcggg gcttccccgg atgaaatcgg
     2761 gaagatcagc gaccaccgcc tcctggcgct ggccgcggat cactaccggg tgctgcagcg
     2821 tctggagcga ggggacagag ccatacagaa gggccgcgac aacagccagc ggacggcaca
     2881 gggcaccacg cccaccatcc gccctgcacc gcgcaaatcc tctcaggccg ctgcacctgc
     2941 caagaagcgg gagctgaagc tgcgcgacaa gatgaaaacc agcgggtcgg tggaagacac
     3001 cgccgcttgg attggagggt cagcacatgg ccgttaatgc caacaccgtc gagacctatg
     3061 atgtctcgac catccgcgaa gacctgcagg aagcgctggt ctcgatctcc cccgaggaat
     3121 gcccgttcat gcagaatgcc gggcagcgca gcgtctcaca gtcgctgttc gaatggcctg
     3181 tcatggaact ggccgccacc gatagctcga accgggtcat cgagggcgac gacgatcctg
     3241 cggtcgatgc cggcacgatc gccggccgcg cgcagaacta ctgccagatt tccgacaagg
     3301 tggtgtccgt ttcgcacacg gacgacgcct ctgacggcgc cggtgacgac caccaccgct
     3361 tcaacaagca ggtcgccatc aaattgaggg agttgaagcg cgatatggag gtcatgctcc
     3421 tgggggctgt ggtcgcggcg cccgggtcga gcggcgttgc ccgctcgacg gcgggtcttc
     3481 cggcgtggct gaagaccaac actagccgcg gcgccggtgg caccgacccc accgtctccg
     3541 gggccggtga cttcgacggt taccccaacg ccgcccccgg cgccggcacg ggccgagcgc
     3601 tcaccgagac catcttcaac gatgtgatcg agctgatctg gaacgagggc ggcgagccgg
     3661 gcatggtgct ctgcaatggc gcccggaagc ggaagatcag cacgttcacc ggcaatgcga
     3721 gccggtacaa gaacgccgag gatcgccgcg ttgtggccgc gatcgacctc tacgcgtcgg
     3781 acttcggctt cgacatccag atcgtgcctg acaggttcat ggcggttgcg accgacgtgt
     3841 atctgtgcac gccgggcatg taccggttgg cgtacctgga gaagaccaag cagaaggagc
     3901 tggccgagac cgggcactcc cgcaagcgac tggtctggaa cgagtacggc ctgcaggtgg
     3961 acaacgagaa ggcacacggc gtcatcgccg acatcacctc gtaagcccaa caggggggcg
     4021 cttcggcgcc cccttgaccc cggaaacaca tcaggagctg cccccatgcc cgagacctgc
     4081 tggatcgtca accgcgcgcg atcgaccgcg cacgttcccg tgcctcacga tgacccggaa
     4141 gtgcacgacc cccgccgcat cgcatctggc gactatgaag aagtgcccgg ctccgtggac
     4201 acctggaagg cgcggctgaa gggccatgca catcttgagg tccgggccag ggcgccccgt
     4261 gaaggcgagg acatcctggc cgctgcggtg ctgcccaagc ccccgaagca gcgcggttcc
     4321 acgaaccgca gatccaggga acagatcgat ggggctgctt gatccgcaaa acggttctct
     4381 gcgtgcgcca gatggtcaag tcaccgaccg ctggagcgtc gatcccgaga caggcatagt
     4441 gcatcgcgag caggctgaag acgtggagag cgtcatcgac cacaacgcgc acttgcgtga
     4501 cttcctcccg aagtaccacg gtcacgccgc ctggcggcac tgcgcctcca ttccctccac
     4561 gatcgccaat ctgctctccc gggagtgcgg cgcggccccc gggtcacctg aatttgctgc
     4621 atacgcgaag cggcagatcg tgctacgctt ccctaagctg ctcgtcaaag gatggtgatc
     4681 gaccatggcc ttcacgaccc acgccgagtt caagaccgag atcgaggagt ggctggaccg
     4741 gcctgacctg tccgccaaga tcgacagctt catcgacatc gccgaggcca ccatccgccg
     4801 ccgttgcaag ggtctcgagc gcgaagtgcg cgcccaggcg acggtgactg ccgggcagcg
     4861 ctacgtcgcc ctgccgaccg actaccgcgg catgaagaac ctgtcaattc ggcccgagga
     4921 cggcggaggc tacctggagc agcgcacccc ggacgagctg gaccggctca ccgagcgcaa
     4981 tgcgctcagc tccagcacgt ccacgccgtt ctggtacgcg ctcaatggca acgagctgga
     5041 actcagcccg gtggccggcg cggacatcat cctggagatg acctacattg aaaccctgtc
     5101 gccgatcagc cctagcggca ccaatctcgt tttcgaccgg caccccgaca tctatctcta
     5161 cgcgaccctg tgcgcggcgt cctactacac cgtggaggat cgccgcgtgc cgctctggaa
     5221 ggcgtccctg accgaagcgg tcaacgagta caacggcgac gccgagcagc gaaagtacgg
     5281 cgcagcgccg ctgcggatgc ggcccgccct gaaga
//
LOCUS       DBNHQR010000440         2826 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1118279, whole genome shotgun sequence.
ACCESSION   DBNHQR010000440 DBNHQR010000000
VERSION     DBNHQR010000440.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1118279"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            260..1624
                     /gene="ffh"
                     /locus_tag="AC16VR_09940"
     CDS             260..1624
                     /gene="ffh"
                     /locus_tag="AC16VR_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008151618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HIR6011520.1"
                     /translation="MFDNLSERLTQTLRGISGKAKLTEENVQDALRDVRMALIEADVA
                     LPVVKDFLSQVQTRALGQTVSSALNPGEEFVRIVHEELVHVMGDDNDALNLATTPPAV
                     VLMAGLQGAGKTTTVAKLALLLKERERKKVAVVSADVYRPAAIEQLATLAGEVGVICV
                     PSSAQEKPLEIARRAVDEARNQFADVLLVDTAGRLAIDADMMAEIKLLHEELQPIETL
                     FVVDAMTGQDAANTAKAFNDALALTGVVLAKADGDARGGAALSVRAITGKPIKFIGMG
                     ERVDALEPFYPDRMASRILGMGDVLSLIEEAERKVDKRKAQKLAKKIQRGRKFDLEDF
                     REQLDQMSNMGGVSSMLEKLPGMGQLPPAAQAQLDDGMFRKMAVIIDSMTPHERRFPD
                     VITGSRKQRVARGSGTQVPDVNRLLKQHKQMQRVMKKMSRKGGMQRMMRGMQGMRPPP
                     GVRR"
     gene            1723..1995
                     /gene="rpsP"
                     /locus_tag="AC16VR_09945"
     CDS             1723..1995
                     /gene="rpsP"
                     /locus_tag="AC16VR_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HIR6011521.1"
                     /translation="MLTIRLARHGSKKNPFYHITVADRTAKRDGRFVERVGFYNPVAK
                     GQAEELRVDLERIDYWLSVGALASDPVTRLIRRARRQEAAVEATAD"
     gene            2052..2555
                     /gene="rimM"
                     /locus_tag="AC16VR_09950"
     CDS             2052..2555
                     /gene="rimM"
                     /locus_tag="AC16VR_09950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HIR6011522.1"
                     /translation="MHSQEYVIVGRFGGPYGVHGWIRVTSFTDPPANLLLYGPWHVER
                     DGQWHAYHPVQVRPHQQGLIACYADIPDRSVAAALTGRFIAVPRDCLPVLDAEEYYWR
                     DLEGLEVWNRGARLGVVDHLIDTGANSVLAVRGDSTQTLIPFVSRFVTGVDLAAGRID
                     VDWEEVD"
     gene            2558..>2826
                     /locus_tag="AC16VR_09955"
     CDS             2558..>2826
                     /locus_tag="AC16VR_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014576266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011523.1"
                     /translation="MWCGIVSLFPEMFRRLSELGVLGRAIDRGIVTLELFNPREFARD
                     RHRTVDDRPYGGGPGMVMQVEPVVAAVSAAREAARAALGESVSVI"
BASE COUNT          508 a          885 c          954 g          479 t
ORIGIN      
        1 tccccactgc gatgagcggc tgcaacaaca cgggagccga tcgcgacagt cgtcgcagct
       61 gcatcgcggt gcccaggagg tacagcaaca cggcgcacgt tccggcggcg atgctcgtag
      121 ttgtggcaac catttcggtc ggcgtcgtcg atggctggag cgtcggggag tttggcataa
      181 cgcaccaata gcgtgaaggg ctataatcgg cccgccgccg atggtcccgg cgtgcgtcca
      241 acggctcgac gaggttttca tgttcgacaa tctttccgaa cgacttaccc agacgctccg
      301 cggcatctcg ggcaaggcga agctcacgga ggaaaacgtc caggacgcct tgcgcgacgt
      361 gaggatggcc ctcatcgagg ccgacgtcgc gttgccggtg gtgaaagact ttctctccca
      421 ggtccagacc agggccctgg gccagacggt ttcgtctgct ctcaatcccg gcgaggagtt
      481 cgttcggatc gttcacgaag agctagtcca cgtgatgggc gatgacaacg acgccctcaa
      541 cctggccacc acgcccccgg ccgtcgtgct catggccggc ctgcagggcg ctggcaaaac
      601 gaccacggtt gccaaactcg cgctgctcct gaaagaaagg gagcgcaaga aggtggcggt
      661 ggtcagtgcc gacgtttacc gtccggctgc catcgagcag ctcgcgaccc tggccgggga
      721 ggtcggcgtg atctgtgttc catccagcgc gcaggagaag ccgctggaga tcgcccgtcg
      781 tgccgttgat gaagcccgca accagtttgc cgacgttctg ctcgtggaca ccgcgggacg
      841 ccttgcgatc gatgccgaca tgatggcgga gatcaagctg ctgcacgaag agctccagcc
      901 catcgagacg cttttcgtcg ttgacgccat gaccggtcag gacgcggcca acacggccaa
      961 ggctttcaac gatgcgttgg cgctaaccgg cgtcgtgctg gcaaaggccg acggggatgc
     1021 ccggggcggt gccgccctgt cggtccgcgc catcacgggc aagcccatca agttcatcgg
     1081 catgggcgaa cgggtcgatg ccctggagcc tttctatccg gaccgcatgg catcgcgaat
     1141 cctcggcatg ggcgatgtcc tcagcctcat cgaggaagcc gagcgcaaag tcgacaagcg
     1201 caaggcacag aaactcgcca agaagattca gcgtggtcgc aagttcgacc tggaggactt
     1261 ccgcgagcag ctcgatcaga tgtccaatat gggaggcgtt tcgagcatgc tggagaagct
     1321 gccgggcatg gggcagctgc cgcccgcggc gcaggctcag ctggacgacg ggatgtttcg
     1381 caagatggcg gtgatcatcg actccatgac cccccacgaa cgccgcttcc cggacgtgat
     1441 caccggatcc cgcaagcagc gggtcgcgcg gggcagcggc actcaggtgc cggatgtgaa
     1501 ccgcctgctc aaacagcaca agcagatgca gcgggtgatg aagaagatgt cgcgcaaagg
     1561 cggcatgcag cgaatgatgc ggggcatgca ggggatgcgg ccgccgcccg gcgtgcgtcg
     1621 gtgatggagc ctcaccggcc ttgcatgagc cgggcgttcc attaaaatac cgcgcctttc
     1681 aacgattggg cccgcctggc gggcccgcag cagggtcatc acatgctaac gattcgcctg
     1741 gcacgccacg gatccaagaa gaaccccttc tatcacatca cggtcgcgga tcgcacggcg
     1801 aagcgagacg gcagattcgt cgagcgggtc ggcttctaca atccggtggc caagggtcag
     1861 gccgaggaac tgcgcgttga tctcgagcgg atcgactact ggctgtccgt tggcgcgctc
     1921 gcgtctgatc cggtcacccg gctgattcgt cgggcacggc ggcaggaagc cgccgtggaa
     1981 gccaccgccg actgacgggc cgcgtcgggc gccccacggc gggcgttgcc ggggatcccg
     2041 gagtcttccg catgcattcc caggaatacg tcatcgtcgg ccgcttcggc ggtccttacg
     2101 gtgtgcacgg ctggatccgg gtcacctcgt tcacggatcc gcccgccaac cttctcctct
     2161 acgggccctg gcacgtggag cgtgacgggc aatggcacgc ctaccacccg gtccaagtgc
     2221 ggccccacca gcagggcttg atcgcctgct acgcagacat cccggaccgc agcgtggcgg
     2281 ccgcgctcac cgggcggttc atcgccgtac cccgggactg tctgccggtg ctggacgcgg
     2341 aagagtatta ctggcgagac ctggaaggtc tcgaggtatg gaaccgcggg gcgcggctcg
     2401 gcgtggtgga tcatctcatc gacactggcg cgaactcggt gctggcggtt cgaggcgact
     2461 cgacgcagac gctgattcct ttcgtcagcc ggtttgtaac cggggtcgat cttgccgcgg
     2521 gcaggatcga cgtcgactgg gaagaggtcg actagacgtg tggtgcggca tcgtcagtct
     2581 ttttccggag atgttccgac ggctctccga gctgggcgtg ctcggcagag cgatcgatcg
     2641 aggcatcgtg accctcgagc tcttcaaccc gcgcgaattc gcccgggacc gacaccgcac
     2701 ggtggatgac cggccatacg gcggcgggcc cgggatggtc atgcaggtag agccggtcgt
     2761 tgcggccgtc agcgcggcgc gggaggcggc ccgagccgcc ctgggtgaga gcgtgtcggt
     2821 gatcta
//
LOCUS       DBNHQR010000441         3987 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_991914, whole genome shotgun sequence.
ACCESSION   DBNHQR010000441 DBNHQR010000000
VERSION     DBNHQR010000441.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_991914"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1238
                     /gene="argS"
                     /locus_tag="AC16VR_09960"
     CDS             <1..1238
                     /gene="argS"
                     /locus_tag="AC16VR_09960"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HIR6011524.1"
                     /translation="TQFGMLLTHMTETGADSNTLSDLEDFYREAKARFDADPDFAERS
                     RQTVVRLQSGDPDTRAHWSRFIDISLSHCQAIYDRLGVKLTAADVAAESAYNDALPEV
                     IEALRASGLLEESDGALCVFLDEFTGKDDQPLPVIVRKSDGGYLYATTDLAAIAHRSR
                     TLHADRAMYFTDARQALHFRQVFAVARTAGLAAPEMRLDHLPFGNMLGKDGTPFKTRE
                     GGVVRLEELLDEAEVRAYALVGEKSPDLPETERRQIARAVGIGAIKFADLSKNRTSDY
                     VFDWDQMLSFEGNTAPYLQYAYSRIRSVFRRGDVQPDALDTPARVEMSTPEERGLAVM
                     LVRFQEVLEQVATEACPHFLCAYLYELASAFTRFYEACPILTADEPLRASRLFLADRT
                     ATTLEQGLGLLGIETMERM"
     gene            complement(1243..2448)
                     /locus_tag="AC16VR_09965"
     CDS             complement(1243..2448)
                     /locus_tag="AC16VR_09965"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019340796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DesA family fatty acid desaturase"
                     /protein_id="HIR6011525.1"
                     /translation="MSVILEWLSGGILNLSVLGMVIFTLVVTHVTIVAVTVYLHRHAA
                     HRSVEVHPALGHFFRFWLWLTTGMITREWASIHRKHHAVCETEEDPHSPKIKGLGAIL
                     WRGAEAYREASKQREIMDRYGAGCPDDWVERNVYQRFNFLGIVLMLIIDVALLGVAGI
                     TVWAVQMIWIPFFAAGVINGIGHYWGYRNFECPDAATNISPWGILIGGEELHNNHHTY
                     PNSAKLSQKPWEFDIGWFWIRLFETFGLARPLSTGPVVERVSGKTAIDVDTAWAVLND
                     RFRVMARYAEEVVAPLVEQEYHRADHATRALFKKAKKTLCREDSLVDAAGKTRIAELI
                     GHSPEIREIYEFRLRLQDVWAKRAAGADALLNELKQWCADAEATGIEVLRDFVEELKS
                     YSIPRLARA"
     gene            2665..3477
                     /locus_tag="AC16VR_09970"
     CDS             2665..3477
                     /locus_tag="AC16VR_09970"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014852133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000098 - sulfur amino acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HIR6011526.1"
                     /translation="MFTTLISADDLTSRLESGGDGLVVLDCRARLGEPDYGRRAYAAG
                     HLPGAVHVDLDADLAAPPGAGGRHPLPDRAELAAKLRERGLNDADQVVVYDDAGGAFA
                     SRAWWCLRWLGHRAVALLDGGLEQWPGPLSSQLPDVTPGDFSLRPPLTRTIDADRLAA
                     SLATLTLVDARTRARFEGREELIDPVAGHIPGAVCLPFGENLDSDGRFLDARTLARRF
                     EGLGADIVCYCGSGVTAAHNVLAMTIAGLDEPILYPGSWSEWLVDPTRPRAP"
     gene            3507..>3987
                     /locus_tag="AC16VR_09975"
     CDS             3507..>3987
                     /locus_tag="AC16VR_09975"
                     /inference="COORDINATES: protein motif:HMM:NF017266.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MnmC family methyltransferase"
                     /protein_id="HIR6011527.1"
                     /translation="MTWRDRQPYSAAFGDIYHDGTQAEEVNRVFVAPSRLVEKAREQR
                     SLVVGELGFGTGLNFVVAAAACLQASARLHFVSFEAAPISPPEFAALSASRAGQHPLY
                     GELGRQYPPLVRGWHRRVLSNGQITLSLYWGDAEAGLDDIDGRQLRPFDVWFLDGFAP
                     "
BASE COUNT          680 a         1362 c         1274 g          671 t
ORIGIN      
        1 gtacccagtt cggcatgctg ctcacacaca tgaccgaaac cggcgcggac tcgaacaccc
       61 tttcggacct ggaggacttc taccgggaag ccaaagcacg cttcgatgcc gaccccgact
      121 tcgccgagcg cagccgccag accgtggttc gtctgcagtc gggagacccg gacacccgcg
      181 cccactggtc ccggttcatc gacatttcgc tgtcacactg ccaggcgatc tacgaccgcc
      241 tgggcgtgaa gctcacggcg gccgacgtgg cagcggagag cgcctacaac gacgcgctgc
      301 ccgaggtgat cgaagcgctg cgcgcaagcg gccttctcga ggagtcggac ggggcgcttt
      361 gcgtgtttct cgacgagttc acgggcaagg acgatcagcc gcttcccgtg atcgtgcgaa
      421 agtcggacgg cggctatctg tacgcgacca ccgacctcgc agccatcgcg caccgcagcc
      481 ggacactgca cgcggatagg gccatgtatt tcaccgacgc acggcaggcg ttgcactttc
      541 gccaggtctt cgcggttgcc cgcaccgcgg ggctcgccgc cccggagatg cgcctggacc
      601 acctcccctt cggaaacatg ctcggcaagg acggcacgcc gttcaaaacc agagaaggcg
      661 gcgtggtacg actcgaggag ctgctcgacg aggcggaggt gcgggcttac gccctcgtgg
      721 gcgagaagag tcccgatctt ccggaaaccg agcggcgtca gatcgcccgg gcggtgggca
      781 tcggcgccat caagttcgcc gacctgtcca agaaccggac gagcgattac gttttcgact
      841 gggatcagat gctttctttc gaggggaaca ccgcccccta ccttcagtac gcctactcac
      901 gaattcgcag cgtgttccgg cgcggcgacg ttcagcccga tgctctggac acgccggccc
      961 gcgtagagat gagcacgccc gaggaacgtg ggctcgccgt catgctggtg agattccagg
     1021 aagtgctcga gcaggtagcg accgaagctt gcccgcattt cctgtgcgcg tatctctacg
     1081 agctggcgag cgcgttcacc cgcttctacg aggcctgccc catcctgaca gccgacgagc
     1141 cgctgcgcgc gtcgcgtctg ttcctggcgg accggacggc cacgaccctc gagcagggac
     1201 tcggcctgct cgggatcgag actatggaga gaatgtgagg cgtcaggccc gggccaggcg
     1261 cggaatcgag taggacttca actcttcgac gaagtcccgc agcacctcaa tgccggtcgc
     1321 ttcggcatcc gcgcaccact gcttgagctc gttgaggagc gcgtcggcac cggccgcccg
     1381 cttcgcccag acatcctgca accgcagcct gaactcgtag atctccctga tctcggggct
     1441 gtgcccgatg agctcggcga tgcgcgtctt gccggcggcg tccacgagag agtcctcgcg
     1501 gcaaagcgtc ttcttggcct tcttgaacag cgcgcgggtc gcgtgatccg cgcggtggta
     1561 ttcctgctcc accagcggcg cgacgacttc ctcggcatag cgagccatga cgcggaagcg
     1621 atcgttgagc accgcccagg ccgtgtcgac atcgatggcg gtctttccgc tgacccgctc
     1681 taccacaggg cccgtgctca gcggacgggc aaggccgaac gtctcgaaga gccggatcca
     1741 gaaccagccg atgtcgaatt cccatggctt ctgagaaagc ttggccgaat tcggataggt
     1801 gtgatggttg ttgtgaagct cctcgccgcc gatgaggatg ccccagggcg agatgttcgt
     1861 cgccgcgtcg gggcactcga aattccgata gccccagtag tgtccgatcc cgttgatcac
     1921 cccggccgcg aagaagggga tccagatcat ctggaccgcc cacacggtga tgccggcaac
     1981 gccgagcaag gccacgtcga tgatcagcat cagcacgatg ccgagaaagt tgaatcgctg
     2041 gtagacgttg cgctcgaccc agtcatccgg gcaccccgcg ccgtagcgat ccatgatttc
     2101 gcgctgcttg gaggcttccc gataggcctc cgcgccgcgc cagagaatgg cgccaagccc
     2161 tttgatcttg gggctgtgcg gatcttcctc ggtctcgcag accgcatggt gcttgcggtg
     2221 aatggacgcc cactcgcggg tgatcatccc cgtggtgagc cacagccaga atcggaagaa
     2281 atgccccaga gccgggtgca cctcgacaga gcggtgcgcc gcgtgccggt gcaggtagac
     2341 ggtgaccgcg acgatggtca cgtgagtgac gacgagggtg aagatcacca tgcccaggac
     2401 ggacaggttc aaaatgccac cactgagcca ttccagaatc acgctcacgc ctacagacct
     2461 ccttgtgcac agctcccccg ggagcagatc gcgcggcatt gtgacatagg ctgctcgaat
     2521 tgccaccttg cctatcaacg cctaccagta taagaccgcc gcaacgggca aaggttacat
     2581 ccgctgcgac atgagacctc catcgtgcta aaaggcagcc tctgagagcc agttcacgat
     2641 atccacccgg caacgccctg ggccatgttc accactctga tttcggctga cgatctgacc
     2701 tcccgcctgg agagcggggg cgacggtctc gttgtgctgg actgccgcgc acggctgggc
     2761 gaaccggact acggccgccg cgcgtacgcc gcagggcatc tccccggagc cgtgcacgtg
     2821 gacctggatg cggacctggc cgccccgcca ggggccggcg gccgccaccc cctgccggac
     2881 cgggcggagc tggccgcgaa gctcagggag cgcggactga acgatgccga tcaggtggtg
     2941 gtttacgacg atgccggcgg cgcgttcgcc agccgggcct ggtggtgcct gcgctggttg
     3001 ggccaccgcg ccgtcgccct tctggacggc ggcctcgagc agtggcccgg accgctctcc
     3061 tcccagcttc ccgacgtcac gcccggagat ttcagcctgc ggccgcccct gacccggacg
     3121 atcgatgcgg accggctcgc ggcatcactg gccaccctta ccctggtaga tgccagaacg
     3181 cgcgcgcgtt tcgagggccg cgaagagctc atcgatccgg tcgccggcca catccccggc
     3241 gccgtctgcc tgccctttgg tgagaacctg gacagcgacg gcagatttct cgacgcccgg
     3301 acgctggccc gacgcttcga agggctgggg gcagacatag tctgctactg cggctccggc
     3361 gtcacggccg cccacaacgt gctggccatg accatcgcgg ggctcgatga gccgatcctc
     3421 tatcccggct cctggagcga gtggctcgtc gaccccaccc ggccgcgggc accttgacgc
     3481 gtcgggagac cctagctcct ggcgttgtga cgtggcgcga ccgccagccc tactccgccg
     3541 cgttcggcga catctaccat gacggcaccc aggccgagga ggtcaaccgg gtcttcgtcg
     3601 cgccgtcccg actcgtcgag aaggcgaggg agcagcggtc cctggtcgtg ggcgagctcg
     3661 gcttcggcac cggtttgaac ttcgtcgtgg cggcggccgc gtgcctccag gcgagcgccc
     3721 gtctgcattt cgtcagcttc gaagcagccc ccatctcacc cccggagttc gccgcgctga
     3781 gcgcgtcccg ggcaggacag catccccttt acggtgagct cggccggcag tacccgccgc
     3841 tcgtccgtgg ctggcaccgc cgcgtgctga gcaacggaca gatcaccctg tctctctact
     3901 ggggcgatgc cgaggcgggg ctggacgaca tcgacggacg ccagctgcgt ccgttcgacg
     3961 tctggtttct cgacggcttt gcccccg
//
LOCUS       DBNHQR010000442         1715 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_548680, whole genome shotgun sequence.
ACCESSION   DBNHQR010000442 DBNHQR010000000
VERSION     DBNHQR010000442.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_548680"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="AC16VR_09980"
     CDS             <1..374
                     /locus_tag="AC16VR_09980"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6011528.1"
                     /translation="ISGVYGAATQASYAAGKAAVIGLTKSLSKEWGPYNVTVNAVAFG
                     YIETRLTQTIGDEVETIDVKGRQHRVGLTPEAIEAYRSTSPLRRAGAPEDAANAVALF
                     CFPESDFVTGEVLIASGGTTG"
     gene            449..>1715
                     /locus_tag="AC16VR_09985"
     CDS             449..>1715
                     /locus_tag="AC16VR_09985"
                     /inference="COORDINATES: protein motif:HMM:NF013355.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HIR6011529.1"
                     /translation="MTIRFLGKNVSGPFTIPSGIVATAVPIIEYLFRHVPQIGVMTTK
                     SIGLAPRTGNREPIYSQYAPGCFVNAVGLTNPGAERSAELFAALDVPEDRFLLTSIFG
                     GSVEEFVAVAKILAPVSDGLELNLSCPHAKGYGMEMGRDPELVREITAAVKAAVDVPV
                     VPKLTPNADNIGEIAQAAVAGGADALCAINTVGPGYYSAAGEPVLSNELGGMSGKGIL
                     PIGLKCVREIAGRVNVPVIGCGGISSAADVNAYRAAGAHIFGVGSALTGMTSPEITQY
                     FQTLAADLADDGNDAEARIQYRLDMGFRPYTLVTNTRVTDDICILTFDRPLDIKAGEF
                     VFLWIPGLGEKPFSALTDDPFSLVVIDLGQFTHALMDLAPGTRTYVRGPHGLPAEPPP
                     GARIVAVAGGTGLAAVYQLARDHGNADIFM"
BASE COUNT          299 a          586 c          564 g          266 t
ORIGIN      
        1 cgatatcggg ggtgtacgga gccgccaccc aggcgagcta cgctgccggc aaggcggccg
       61 tgatcggcct gaccaagtcc ttgagcaagg agtggggtcc ctacaacgtg accgtgaacg
      121 ccgtggcttt cgggtacatc gagacacgcc tcacccagac gatcggcgac gaggtggaaa
      181 ccatcgatgt gaagggccgc cagcaccggg tgggcctcac gccggaagcc atcgaggcct
      241 accggagcac gagcccgctg cgccgggccg gagctccgga ggacgcagcg aacgccgtcg
      301 ccctgttctg ctttccggag tccgatttcg tcaccggcga ggtgctgatc gccagcggag
      361 gcactacggg ttgacgcgtg gttcgattgg gcgcgccgaa cctgtagaat cccgctccga
      421 atcccgacac cgctgagacc cgcccgcctt gacgatccga ttcctgggca agaacgtatc
      481 cggcccgttc accatcccat cggggatcgt cgcgaccgcc gtgccgatca tcgagtacct
      541 gttcaggcac gttccgcaga tcggtgtgat gaccaccaag agcatcgggc ttgcaccccg
      601 caccggcaat cgcgagccca tctacagcca gtacgcgccc ggctgcttcg tcaacgccgt
      661 gggcctgacc aacccgggcg ccgaacgttc ggccgagctc ttcgccgcgc tcgacgtccc
      721 ggaagatcgc tttctgctca cctccatctt cggcggcagc gtcgaggagt tcgtggcggt
      781 ggcgaagatc ctggcgccgg tctccgacgg cctggagctg aacctgtcct gcccccacgc
      841 caaggggtac ggcatggaga tgggccggga tccggagctg gtgcgcgaga tcaccgcggc
      901 ggtgaaagca gccgtggacg tgcccgtggt gccgaagctc acgccgaacg cggacaacat
      961 cggcgagatt gcgcaagccg ccgtggccgg cggggcggac gccctgtgcg ccatcaacac
     1021 cgtggggccc ggctactaca gcgccgcagg cgagcctgtg ctgagcaacg agctcggcgg
     1081 catgtcgggc aaaggcatcc tgcccatcgg cctgaagtgc gttcgggaga tcgccggaag
     1141 agtcaacgtc cccgtgatcg gctgcggtgg gatcagcagc gcggccgacg ttaacgccta
     1201 ccgcgcggca ggggcccaca tattcggggt gggctcggcg ctgaccggca tgacgtcgcc
     1261 agagatcacc caatacttcc agacgctggc ggcagacctg gcagacgacg ggaacgacgc
     1321 ggaagccagg atccagtacc ggctcgacat gggcttccgc ccgtacacgc tggtgacgaa
     1381 cacccgcgtc accgacgaca tctgcattct caccttcgac cggccgctgg acatcaaagc
     1441 gggtgagttc gtcttcctgt ggatacccgg tctgggcgaa aagcctttct cggcgctgac
     1501 cgacgatccg ttctcgctgg tggtcatcga cctggggcag ttcacccacg cgctcatgga
     1561 tctcgctccg ggtaccagga cctacgtgcg cggtccccac ggcctgccgg cggaaccgcc
     1621 cccaggcgcg cgcatcgtcg cggtcgccgg cggtacgggc ctcgccgccg tctaccagct
     1681 ggcaagagac cacggcaacg ccgatatctt catgg
//
LOCUS       DBNHQR010000443         4808 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_612392, whole genome shotgun sequence.
ACCESSION   DBNHQR010000443 DBNHQR010000000
VERSION     DBNHQR010000443.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_612392"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /locus_tag="AC16VR_09990"
     CDS             <1..1213
                     /locus_tag="AC16VR_09990"
                     /inference="COORDINATES: protein motif:HMM:NF014998.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase
                     domain-containing protein"
                     /protein_id="HIR6011530.1"
                     /translation="DLVPVHDLKSGLFLPRLAPAERVDVEDHLHLVTLPAGSGEQALL
                     ELAQRLHAECLDRSRPLWDVYLITGFGERCFAMFVKIHHAIFDGHGGLRLFVETLEEA
                     PVTRLPEPFWSRPSAAAPGHGRPLGPFEAVGALARSAGGLFRNAAVGARHASRWLLGS
                     APVAPPAFSEPASILNGPSSGRRRLAHLSLPLPEIERCAADHGASVNDVLLAICGGAL
                     RRYLEARSALPDRSLTALVPCSLRADAGRLPAGNRLTFIPMPLGTDIAEHPDRVSAVR
                     AGAGEAKERLEELGDPGALQYGMLINAVATLPRLARLPLPPISPANLVVTNLAGPAAP
                     RHLGSARMTQIYAHAPMSDGVGLNIALVSYAGEAFLGLTADPSLVDDVDELARLLART
                     ASELLGGSEAA"
     gene            complement(1214..2143)
                     /gene="nudC"
                     /locus_tag="AC16VR_09995"
     CDS             complement(1214..2143)
                     /gene="nudC"
                     /locus_tag="AC16VR_09995"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HIR6011531.1"
                     /translation="MSHVHIFAGNPLDRADALRRDPEWLDGARADPGSRYLPFARLNV
                     LLCGERPELGWLRSSEVQRLGISVPPIFLGLREGIAHFAIDVSELGDPAHELNLDDSW
                     RFEDCRAAGTVLSVPDSGIVAQSRSQIDWHRRHQFCSVCGSRSEPERGGHVRRCPSCS
                     AEHFPRTDPVAIMLITNGDECLLGQAAGRLSRLGMYSALAGFIDQGESIEEAVRREVR
                     EEAGVRVGAVRYHSSQPWPFPSSLMIGCHGTALSTDITIDPSEMADVRWFSRDSVRLA
                     LQDDNPELRVPGPIAIAHHLIRAWADGEVDLDT"
     gene            2355..2636
                     /locus_tag="AC16VR_10000"
     CDS             2355..2636
                     /locus_tag="AC16VR_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011532.1"
                     /translation="MISLTFYGLACLCGIAAVLILLVGFVDWLQTERWTNLSVLEFGY
                     DAHLIRARWFINNRWSWWLHDLMHLIPLYVFLLALAPLSWVVGAWFGRR"
     gene            complement(2661..3017)
                     /locus_tag="AC16VR_10005"
     CDS             complement(2661..3017)
                     /locus_tag="AC16VR_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011533.1"
                     /translation="MSERDEQEKLEEQACALLLRSPHLSVSQVLDLMDIGDSEFREML
                     RSNPRIASLMEQRREGALPRVESKMTECPACGEWFIPYASARCCSDTCAKIDQISNRP
                     NAARRFRLRNRQTGGS"
     gene            complement(3153..>4808)
                     /locus_tag="AC16VR_10010"
     CDS             complement(3153..>4808)
                     /locus_tag="AC16VR_10010"
                     /inference="COORDINATES: protein motif:HMM:NF005863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HIR6011534.1"
                     /translation="HVIVSEARAMTYPWNYGDLLDAVEHITPGSRAALIHGDRIVSWA
                     DFSRRTNNLARALLNGGAAPGDKIAFYMRNRPEYSEGIAAGFKARLTHVNVNYRYVDH
                     ELIYLLDNSDAAVVVYAAEFAHHVAAIRDQLPGVGQWIEVPDGHPGLDDALDYERLVA
                     DGDGAPLDLDRSPDDMLFLYTGGTTGMPKGVMWRHQDLWCVTGAGGNPRIGVEPSPDL
                     QSYVSRLQAEPPPVNLPLPPIMHGTGLLSVVGAMAHGGTAVTLTGRSFEAEAALEAIQ
                     RHAVTSTTIVGDAFARPMVEALDAAPGRHDISSLKIVSSSGVMWTRAIKAGLLRHNPD
                     LVLVDAFSSSEAIGLGTSVMTRDEEIEVAKFTLGPSCKVFGEDLREIAPGSGETGMVG
                     VTGFLPVGYYKDEEKTARTFPVVDGVRYSIPGDWVRVEADGSLTLLGRGSNCINTAGE
                     KVYPEEVEEALKHHESVADALVVGLPDERWGQAITAVITLHPGYSLDEIGLREFARRE
                     LAGYKLPKRILAKDDLERAPNGKANYAAIREFAQAELGISSGS"
BASE COUNT          750 a         1607 c         1596 g          855 t
ORIGIN      
        1 cgacctggtt cccgtgcacg atctgaaaag cgggctcttt ctgccgcggc tggccccggc
       61 cgagcgcgtg gacgtggagg atcatctgca tctggtaacc cttccggccg ggagtggcga
      121 gcaggcgctc ttggaacttg cccagcgcct ccatgccgag tgtctcgacc ggagccgccc
      181 gctctgggac gtgtacctga tcaccggctt cggcgagcgc tgtttcgcga tgttcgtgaa
      241 gattcatcac gcgatcttcg acgggcacgg cggcctgcgc ctgttcgtgg agaccctgga
      301 agaagccccc gtgacgcggc tgccggagcc gttctggtcc aggcccagcg ccgcggcccc
      361 cggccatggc cgccctctgg gtccgttcga ggcggtgggt gccctggcgc gttcggccgg
      421 cgggctcttc cggaacgcgg ccgttggcgc gcgccatgcc tcccgctggc tgctcggttc
      481 ggcgccggtg gccccgcccg cattcagcga gccggcgagc atcctcaacg ggccctccag
      541 cggtcgacgc cgactggccc acctgagcct gccgctgcct gagatcgagc gatgtgcggc
      601 cgaccacggg gcatccgtca acgacgttct gctcgccatc tgcggcggcg cgctgcgccg
      661 ctatctcgaa gcgcgcagcg ccctgccgga ccgctcgctc accgcgctgg tgccctgctc
      721 gttgcgggcc gacgccggcc ggctgccggc cgggaaccgg ctcacgttca tacccatgcc
      781 gcttggcacc gacatcgccg aacatcctga ccgcgtttcg gcggtccgtg ccggggcggg
      841 cgaagccaag gaaaggctcg aggaactggg cgaccctggc gccctgcagt acggcatgct
      901 catcaatgcc gtggcgaccc tgccgagact cgctcgcctc ccgctgccgc ccatctctcc
      961 tgccaacctc gtggtcacga acctggcggg cccagctgcg ccccgtcatc tgggaagtgc
     1021 gcggatgacg cagatctatg cccatgcccc catgtccgat ggcgtaggtc tcaacatcgc
     1081 cctggtttcc tatgccggtg aagccttcct cgggctcacc gcagatccct cgctggtgga
     1141 cgacgtggac gagctggccc gactgctggc tcggaccgcc agtgagcttc tcggcggaag
     1201 cgaggcagcc tgatcaggtg tcgaggtcga cctcaccgtc ggcccaggct ctgatgaggt
     1261 ggtgggcgat ggcgatgggc ccgggcaccc tgagctccgg gttgtcgtcc tggagcgcga
     1321 gccggacgga gtcgcgggag aaccagcgca cgtctgccat ctcgctggga tcgatggtga
     1381 tgtccgtgct cagcgcggtt ccgtgacagc cgatcatcag cgacgaggga aacggccacg
     1441 gctgcgagga gtggtagcgc acggcgccca cccggacgcc ggcctcctct cgaacctccc
     1501 ggcgcacggc ttcctctatg gattcgccct gatcgatgaa tcccgccagg gccgagtaca
     1561 ttcccagccg cgacaggcgt ccggcagcct gcccgagcag gcactcgtcg ccgttggtga
     1621 tcagcatgat ggccaccgga tcggtccggg gaaagtgctc ggcgctgcac gaggggcagc
     1681 gacggacgtg gccgccccgc tccggctcgc tgcgggaacc acacacgctg cagaactggt
     1741 ggcgtctgtg ccagtcgatc tgggatcgac tctgagccac gatgccgctg tccgggacgg
     1801 aaagcacagt gcctgcggcc cggcagtcct cgaaacgcca ggagtcgtcc aggttcagct
     1861 cgtgggccgg gtctccgagc tcggagacgt cgatcgcgaa gtgggcaatg ccctcccgca
     1921 agcccaggaa gatggggggc acgctgatgc ccagccgttg cacctcgctc gacctcagcc
     1981 agccgagctc cggccgctcc ccgcacagca gcacgttcag ccttgcaaag ggcagatagc
     2041 gcgagccagg atcggctctc gcgccgtcga gccactcggg atcgcgccgc agcgcgtccg
     2101 cgcggtccag gggattgcca gcaaagatat gaacgtggct cacggatctt ccaggtaagg
     2161 gtcaggtgga tgaaaggaga agggtaaaga ccttgcgcgc attgtgaaac cgccgtcctc
     2221 gagccgttct ggaacgggtt cggcttcccg ggattcgtga aagccgtcac ggcatcgcct
     2281 tcgcgcatcc ggaactctac gcttgggtca atggttcatc ttcagacgga tcgactggca
     2341 ccatggctga tcgcatgatt tccttgactt tctacgggct cgcttgtctc tgcgggatcg
     2401 cggcggtgct gattcttctg gtcggtttcg tcgactggct gcagacagag cgctggacca
     2461 atctaagcgt ccttgagttc ggctacgacg ctcatctcat cagggcgcgc tggttcatca
     2521 acaaccgctg gagctggtgg ctccacgacc tgatgcacct gatcccgctc tatgtgtttc
     2581 ttcttgctct ggcccccctg tcctgggtgg tgggagcctg gttcggtcgg cgctgaaccc
     2641 ccgactgggc gtggtcgacg tcaggagccg ccggtctgcc ggttgcgcag acgaaagcgt
     2701 cgcgccgcgt tgggcctgtt gctgatctgg tcgatcttgg cgcaggtatc cgagcagcag
     2761 cgcgcagacg cgtagggaat gaaccactct ccgcaggcgg ggcactccgt catcttggac
     2821 tccaccctcg gcagagcgcc ctcccgacgc tgctccatga gcgaggcgat gcgcggattg
     2881 ctcctgagca tttcgcgaaa ttcggagtcg ccgatgtcca tcaggtcgag aacctggctg
     2941 acgctgagat gcggagaccg cagcaggagg gcgcacgcct gctcctcgag tttctcctgc
     3001 tcatcccttt cgctcacgcg gccaatactc ctgccggcag atgactcaat cggtgtcggc
     3061 cggccgggtc acggccagtc gtgcgctcat gtcgccgcgc aatgttgccc tgggtaaatg
     3121 acgatcgcct acggcccgtc ctccagacgg tattacgaac ccgatgaaat gcccagttcc
     3181 gcctgagcaa attcccggat ggccgcatag tttgccttgc cgttgggtgc gcgttcgagg
     3241 tcgtccttgg ccagaatgcg cttgggcaac ttgtaaccgg cgagttcccg acgggcgaac
     3301 tcgcgcagac cgatctcgtc cagggagtag cccggatgga gcgtgatcac ggcggtgatc
     3361 gcctggcccc agcgctcgtc ggggagtccc accaccaggg catccgccac cgactcgtga
     3421 tgcttcagcg cctcctcgac ttcctccgga tataccttct ccccggcggt gttgatgcag
     3481 ttgctgccgc gtcccagcag cgtgagcgac ccatccgcct cgacccggac ccagtcaccg
     3541 ggaatcgagt agcgcacgcc gtcgacgacc ggaaaggtgc gcgcggtctt ttcctcgtcc
     3601 ttgtagtagc ccacgggaag gaagccggtg acgcccacca tgccggtctc tccggatcca
     3661 ggcgcgatct cccgcaggtc ctctccgaaa accttgcagc tcggccccag cgtgaacttg
     3721 gcgacctcta tctcctcgtc gcgggtcatg accgaggtac caaggccgat ggcctccgag
     3781 gaggaaaacg cgtcgacgag aacgaggtcc ggattgtgac gcagcaggcc ggccttgatg
     3841 gcccgcgtcc acatgacgcc cgaggagctc acgatcttga gcgacgagat gtcgtggcgg
     3901 ccgggtgcgg cgtccagcgc ttccaccatg ggccgggcga aggcgtcgcc gacgatggtc
     3961 gtggacgtca ccgcgtgccg ctggattgct tccagcgcgg cttccgcttc aaagctccgg
     4021 cccgtcaggg tcaccgccgt tccgccgtga gccatggcgc ccaccacgga tagcagcccc
     4081 gtaccgtgca tgatgggagg cagcggcagg ttgacgggcg gcggctcggc ctgcagacgg
     4141 gacacgtaac tctgaagatc gggggacggc tccacgccga tccgcgggtt gccgccggcg
     4201 ccggtgacgc accacagatc ctggtgacgc cacatgacgc ccttgggcat gcccgtggta
     4261 ccgccggtgt agagaaacag catgtcgtcc ggggagcggt cgagatccag gggcgcgccg
     4321 tcgccgtcgg cgacgaggcg ctcgtagtcg agagcgtcgt cgaggccggg gtggccgtcg
     4381 ggtacttcga tccactgccc gacccccggc agctggtcgc gaatggcggc aacgtggtgg
     4441 gcgaactcgg cggcgtagac gaccactgca gcatcggagt tgtcgagcag gtaaatcagc
     4501 tcgtggtcga cgtagcgata gttgacgttc acgtgagtga gccgggcctt gaagccggcg
     4561 gcgatgccct cgctgtactc gggccggttt cgcatgtaga aggcgatctt gtcgccgggc
     4621 gccgctccgc cgttcagcag cgcccgggcc agattgttgg ttcttcggga gaaatcggcc
     4681 caggagacga tgcgatcgcc gtggatcagg gcggctcgtg accctggcgt gatgtgctcg
     4741 accgcgtcga gcaggtcacc gtagttccac ggataggtca tcgcccgtgc ctcgctaaca
     4801 atgacgtg
//
LOCUS       DBNHQR010000444         3989 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1625523, whole genome shotgun sequence.
ACCESSION   DBNHQR010000444 DBNHQR010000000
VERSION     DBNHQR010000444.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3989)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1625523"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="AC16VR_10015"
     CDS             <1..671
                     /locus_tag="AC16VR_10015"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR6011535.1"
                     /translation="VIKEETNALDDIAQIRAGALSTLNLGMSSMFNRLFGRELLLEFC
                     ERNPLTRLKLDVAPSRELIYGVDEDRWELGIGPFQTLMPGHFETKRFFEEERVLVAHE
                     KHPRFDALMSQPARELAQLTLLTSYLDDATKRPGSERLRNQFASVWEISSLELRLTLA
                     EAGMGVTYVSDRLVGDLEGFHPIDHMEISTIPRVVGIFHKKHVALSEGAKRFLAICQR
                     RFAG"
     gene            complement(682..1290)
                     /locus_tag="AC16VR_10020"
     CDS             complement(682..1290)
                     /locus_tag="AC16VR_10020"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HIR6011536.1"
                     /translation="MKIQERMRAVLQAEAEAIAAVRIDETFEHAVRALLECRGKVITT
                     GIGKAGHIARKFAATLCSGATPADFIHPAEAAHGDLGLVGPDDVMIAFSTSGKSREVL
                     EILELARHLGLAMVIGITSHPDSELRDLSDLVLDMGEIEEPCPVGLTPSASTAVMLAI
                     SDAIALALMEAKGITKEDWGLRHHGGYLGRAARADNVPHDGG"
     gene            1424..3439
                     /locus_tag="AC16VR_10025"
     CDS             1424..3439
                     /locus_tag="AC16VR_10025"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012931.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004563 - beta-N-acetylhexosaminidase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HIR6011537.1"
                     /translation="MPYIAGPEGALLPLPRRFAPGPGGPGAVGVVDARFADASSARLV
                     RAVERLNDRLGRRNGEAGLKMALEISCAIPCPEHPQLGQPEAYQLNVTDQGARLVAET
                     EWGVLHGLETLFQLATAPGGLGCVAVDDAPRFPWRGLLLDVARHYLGMDALLRTLDAM
                     AQVKLNVLHLHLTDDQAFRFPSERFPELASPRHYSRRELAELINHAADRGIRVVPEID
                     VPGHTTCWLVARPDWGTGVTTSTRRFGVHEACLDPTSEAVYAALAELLGEVAEVFPDR
                     HLHLGGDEVHPAWWSASDRVQTFMSERGMTGLDDMQAHFNSRVARTVSGLGKRPMGWD
                     EVVHDDLPDDVVVQSWRGATARDRALGSGHDCVVSSGYYLDLFYPADVHYRFDPQAPE
                     SELVGLEDALLDDPRFEHVAEGMRWTEHWREPAGAAGAAPGSVLGAEACLWGELVTEE
                     LLDVRLWSRLPALAERFWSPRSVRDLGSLYQRLGSCFEQLAAGSGVDVRAASRALMAR
                     AGADEDVVELLELLEPVKWYGRLLGEEALAARIAGTEMPQARPYDADTPLDRVVDALF
                     PESLPARRVARLCQRHFEGDTGAVAGILDCALAWQDLGPRPDCPADVAPALELLCRVG
                     EAVVDVLEGCMAPSAALSLLETAARPQGEYLIAVVPPLRSWLAGLGG"
     gene            3436..>3989
                     /locus_tag="AC16VR_10030"
     CDS             3436..>3989
                     /locus_tag="AC16VR_10030"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR6011538.1"
                     /translation="MIDPLQVARSGWRVGKEVDRLGAGHINDTYLVTDGVDRWVLQRI
                     NDQVFTDPALLMRNLGRVVEHVCARAPGFVPGLLSCRDGSNVFVDEEGGSWRLTEYVS
                     GTESHRRLESVDQATSAARAFARYQNLLADLPAPPLDDPIPGFMRLDGYLAALDAATA
                     RAAGEHGAVDLLGFVEGRRDLAQVL"
BASE COUNT          581 a         1320 c         1410 g          678 t
ORIGIN      
        1 ccgtgatcaa agaggaaacc aacgcgctgg acgacatcgc gcagattcgc gccggcgcgc
       61 tttcgaccct caatctgggg atgagcagca tgttcaaccg gctgttcggc cgcgagctgc
      121 tgctcgagtt ctgtgagcgc aacccgctga cccggctcaa gctcgacgtg gcgccgagcc
      181 gcgagctcat ctacggcgtc gacgaggatc gctgggagct cggcatcggg ccgttccaga
      241 ccctgatgcc cggacacttc gagaccaaac gattcttcga ggaggagcgc gtgctggtgg
      301 cgcacgagaa gcacccgcgc ttcgacgcgc tcatgagtca acccgcccgc gagctggctc
      361 agctgaccct gctcacctcg tatctggacg acgccaccaa gcgcccgggc agcgagcgcc
      421 ttagaaacca gtttgcgagc gtctgggaga tcagcagtct ggagctgcgc ctgactctgg
      481 cggaggcggg catgggcgtg acctacgttt ccgaccggct ggtcggcgac ctggaggggt
      541 tccatcccat agatcacatg gaaatctcga ccattccccg agtggtgggc atcttccaca
      601 agaaacacgt tgccctttcg gaaggcgcca agcgctttct ggccatatgc cagcggcggt
      661 ttgccggctg attcgagcgg gtcagccgcc gtcgtggggc acgttgtcgg cgcgggccgc
      721 cctgcccaga tagccgccgt gatggcgcaa gccccagtct tccttggtga tgcccttggc
      781 ctccatgagc gccagcgcaa tggcgtcgct gatggcgagc atgacggccg tgctggcgct
      841 gggggtcagc cccaccgggc aaggttcttc gatctcgccc atgtccagca ccaggtcgga
      901 aaggtcgcgt agctcggagt cggggtggga ggtgatgccg atgaccatgg ccaggcccag
      961 gtgcctggcc agctcgagta tttccagcac ctcgcggctc ttgccgctgg tcgagaaggc
     1021 gatcatcacg tcgtcgggtc ccaccagccc caggtccccg tgagcggctt cggcagggtg
     1081 gatgaagtct gccggcgtcg cgccggagca gagcgtggcg gcaaacttgc gggcgatgtg
     1141 acccgccttg ccgatgcccg tggtgatcac cttgccgcgg cactcgagca gggcgcggac
     1201 ggcgtgctca aacgtctcgt cgatccgaac cgccgcaatg gcctcggcct cggcctgcag
     1261 cacggcccgc atacgttcct gaattttcat tgccgacacg gtccgcgatt gcccggcgca
     1321 atggtgtcac gcgcatccgg cgtcctcaag gtgacgccgt gcattgacag cgtctggcgc
     1381 ctcttacaga gtccgggtct cttcgacgtt cgaggcacga cgcttgccgt acatagcggg
     1441 accggaaggg gcgctgttac ccctgccgcg ccggtttgcc ccggggcctg gtgggccggg
     1501 ggcggtgggc gtcgtggatg cccgctttgc cgacgccagc tcggcgcggc tggtacgcgc
     1561 ggtggaacgg ctgaatgacc gtctgggccg gcgcaacggg gaggcgggtc tgaaaatggc
     1621 cctcgagatc tcctgcgcga tcccgtgtcc ggagcaccct cagctgggcc agccggaggc
     1681 ttaccagctc aacgtgactg accagggcgc ccggctcgtg gcggagaccg agtggggggt
     1741 cctgcacggg ctggaaaccc tgttccagct ggcgacggcg ccgggcggcc tcggctgcgt
     1801 ggctgtcgac gatgcgccca gatttccctg gcgcggtctg ctcctggacg tggcccggca
     1861 ctatctcggc atggacgcgc tcctgcgtac gctcgacgcc atggctcagg tgaagctgaa
     1921 cgtcctgcat ctgcacctga cggacgacca ggcgttccgg tttcccagcg agcgattccc
     1981 cgagctcgcc agcccacgac actattcccg acgcgagctc gccgagctca tcaaccacgc
     2041 ggccgaccgc ggcattcgcg tggtgcccga aatcgacgtc cccgggcaca cgacctgctg
     2101 gctcgtggcc cgccccgact gggggaccgg cgtgacgacg tcgaccaggc ggttcggtgt
     2161 ccacgaggcc tgcctggatc ccaccagcga agccgtctac gccgctctgg ccgagctgct
     2221 gggcgaggtg gccgaggtgt ttccggatcg ccatctgcat ctggggggcg atgaggtgca
     2281 tccggcttgg tggtcggcaa gcgaccgcgt tcagaccttc atgtcggagc gcggcatgac
     2341 cgggctcgac gacatgcagg cccacttcaa ctcccgggtg gcccgcacgg tgagtgggct
     2401 tggcaagcgg cccatgggtt gggacgaggt ggtgcacgac gatctgcccg acgacgtggt
     2461 cgttcagagc tggcggggcg cgaccgcgcg ggaccgggct ctgggtagcg gccacgactg
     2521 cgtcgtttcg tcgggctact acctggacct cttctatccg gccgacgtgc actaccgctt
     2581 cgatccgcaa gccccggagt ccgagctcgt cgggctcgag gatgcgctcc tcgacgaccc
     2641 ccggttcgag cacgtggccg agggcatgcg ctggaccgag cattggcgcg agcctgccgg
     2701 cgcagccggc gctgcccccg gttcggttct gggcgccgag gcgtgcctgt ggggcgagct
     2761 ggtcaccgag gagctgctcg acgtgcgcct gtggtcccgg ctgccggccc tggccgagcg
     2821 attctggtcg ccccgctcgg tgcgcgatct ggggagcctt taccagcggc tgggaagctg
     2881 ctttgagcag ctcgccgcgg gctcgggggt ggacgtccgg gcggcgagcc gtgccctcat
     2941 ggcgcgggcg ggagcggacg aggacgtcgt cgagctgctc gagctgctgg aaccggtcaa
     3001 gtggtacgga cggctcctgg gcgaggaagc gctcgccgcc cgcatcgcgg gaaccgagat
     3061 gccccaggcc cggccctacg atgcggacac gccgctggac agggttgtcg atgcgctgtt
     3121 tccggagagc ctgcccgcgc gccgtgtggc caggctgtgc caacgtcact tcgaaggcga
     3181 caccggcgct gtggcgggta tcctggattg cgcgctggca tggcaggacc ttggcccgcg
     3241 gccggactgc ccggccgacg tcgcgcccgc cctcgagctc ctctgccgcg tgggcgaggc
     3301 ggtggtggac gtgctcgaag gttgcatggc gccttccgct gcgctgtcgc tgctggagac
     3361 ggctgccagg ccccagggtg agtatctcat cgccgtggtg ccgcccctgc ggtcgtggct
     3421 cgcggggctg ggcggatgat cgatccgttg caggtggccc gctccggctg gcgagtcggc
     3481 aaggaggtcg atcgcctggg tgccggacac atcaacgaca cgtacctggt gaccgacggc
     3541 gtcgaccgct gggtcctgca gagaatcaac gaccaggtgt ttacggaccc ggccctcctg
     3601 atgcgcaacc tgggccgggt agtggaacac gtttgcgctc gcgcgccggg attcgtgccc
     3661 gggctgctgt cgtgccgcga tggctcgaac gtcttcgtgg acgaggaagg cggcagctgg
     3721 cgcctgacgg agtacgtgag cggaaccgaa agccaccgtc gtctcgaaag cgtcgaccag
     3781 gcaacgtcag cggcgcgtgc gttcgcccgt taccagaatc ttctcgccga tcttccggcc
     3841 ccgccgctcg atgatccgat acccggcttc atgcggctcg acggctacct cgcagcgctc
     3901 gatgccgcca cagcacgcgc ggcaggcgag cacggtgcgg tggaccttct cggattcgtg
     3961 gaggggcgac gagatctggc ccaggtgct
//
LOCUS       DBNHQR010000445         2139 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_549344, whole genome shotgun sequence.
ACCESSION   DBNHQR010000445 DBNHQR010000000
VERSION     DBNHQR010000445.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_549344"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="AC16VR_10035"
     CDS             <1..564
                     /locus_tag="AC16VR_10035"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HIR6011539.1"
                     /translation="AAALMAMFFSMGAAGVFKDQRGTNLLDSGSHFYDTYETKDGKYV
                     CIGSIEPQFYALLVEKAGLDAERFAAQMDASRWPDYKRELTEVFKSKTRDEWCEIMEG
                     SDVCFAPVLSIFEAPDHPHSQARNAFIELDGVVQPAPAPRFSRTEVAASHGARVPGED
                     SVSVLEDCGFTGDEIAGLKNDGVIGGN"
     gene            580..1116
                     /locus_tag="AC16VR_10040"
     CDS             580..1116
                     /locus_tag="AC16VR_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011540.1"
                     /translation="MAVELLDTERFHGFRVRRQIAGKTYQEYFSLKDDGKRIKGPQRA
                     TVKALAEARDAELANLQKAARERADRTVRVDARGQVRGILCRLKTEKSGTLTPVFQIG
                     VMSRLEDKIVNTTVSISRYGETEAWRRAIDFYALHKGISKRSRAYRDLLAAQPSGAKI
                     KSLLKASGRAAPRRSARR"
     gene            1113..>2139
                     /locus_tag="AC16VR_10045"
     CDS             1113..>2139
                     /locus_tag="AC16VR_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016562180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6011541.1"
                     /translation="MSYASEYARSLSDPEGFWREKAAELPWLEFPQTILDRAPSGAWR
                     WFHGGKTNTCWLALDRHVADGRGAQVALIYDSPATGSVQRFTFAELTERVAACAGGLA
                     ALGVSRGDRVIVYMPMIPEAAIAMLACARIGAIHSVVFGGFAAPELATRIDDAEPKVV
                     LSASCGIEFANVIPYQPLVDEAISLAVHKPERVVMLQREQQEADLSGERYLDWASWEA
                     SAQGVGCVPLDATDPLYVLYTSGTTGKPKGVVRDNGGHAVALNYSMEAIYGMDAGDVF
                     WAASDVGWVVGHSYIVYGPLIRGCTSVLFEGKPVRTPDAGVFWRVIQDHRVKCFFVAP
                     TALRAIRK"
BASE COUNT          389 a          669 c          711 g          370 t
ORIGIN      
        1 gcggcggccc tgatggccat gttcttctcc atgggcgctg ccggggtgtt caaggaccag
       61 cgcggcacca acctgctcga cagcggctcg cacttctacg acacctacga gacgaaggac
      121 gggaagtacg tctgcatcgg ctccatcgag ccccagttct acgccctgct ggtcgagaaa
      181 gcaggcctgg acgcggaacg ctttgccgcc cagatggacg cctccagatg gccggactac
      241 aagcgtgagc tgaccgaggt gttcaaaagc aagacgcgcg acgagtggtg cgaaatcatg
      301 gaaggcagcg atgtctgctt cgctcccgtg ctgtccattt tcgaagcccc cgaccatcct
      361 cacagccagg cccgcaacgc cttcatcgag ctggacggcg tcgtccagcc cgcgccggcc
      421 cctcgcttct cccgaaccga ggtggcggcc agccacggtg cccgggtacc cggcgaggac
      481 tcggtatcgg tgctggagga ctgcggattt acaggagacg agatcgccgg gctcaagaat
      541 gacggcgtga tcggcggcaa ctgaccggag gatcggcaga tggcggtaga gttgctggac
      601 acggagcgtt ttcacggatt ccgtgtgcgc aggcagatcg ctgggaagac ctatcaggag
      661 tatttctctc tcaaggacga cggcaagcgc atcaaaggtc cgcagcgggc cacggtcaaa
      721 gccctggccg aagcccgcga tgccgaactc gcgaatctgc agaaagcggc gcgggaaaga
      781 gcggacagga cggtgcgcgt agatgccaga ggccaggttc gcggaatcct gtgtcggctc
      841 aagaccgaga agagcggtac cctgacaccg gtatttcaga tcggcgtcat gtcgcgcctg
      901 gaagacaaga tagtcaacac gaccgtttcg atcagccgct acggcgagac ggaggcctgg
      961 cgccgcgcca tcgacttcta cgcgctgcac aaagggatca gcaaacgatc ccgcgcctat
     1021 cgtgacctgc tggcagcgca accgagcggc gcgaagatca agagcctgct caaggcgtcc
     1081 gggcgcgccg caccgcggcg atccgcccga agatgagcta cgcgtcagag tacgcgcgat
     1141 cgctctctga tccagaaggg ttctggcggg aaaaagcggc agagcttccc tggctcgagt
     1201 ttccgcagac catactggac cgcgcgccgt cgggcgcctg gcgctggttt cacggcggca
     1261 agacgaacac ctgctggctg gccctggaca ggcacgtggc ggacggccgc ggggctcagg
     1321 tcgcgctcat ctacgattcg cccgcgaccg gcagtgtcca gcggttcact ttcgcagagc
     1381 tcacggagcg ggtggccgcc tgcgcgggtg ggcttgcggc cctcggcgtg tccaggggcg
     1441 atcgtgtcat cgtctacatg cccatgattc ccgaggcggc gattgcgatg ctggcctgtg
     1501 cgcggatcgg tgccattcac tctgtggttt tcggcgggtt tgccgcgcct gagctcgcta
     1561 cccggatcga tgatgccgag cctaaggtgg ttctgagcgc gtcgtgcggg atcgagttcg
     1621 ccaacgtcat cccgtatcaa cctctcgtag acgaagccat ttcccttgcc gtccacaaac
     1681 cggaacgcgt ggtgatgctg cagcgcgagc agcaagaagc ggatctttcc ggcgagcgtt
     1741 atctggactg ggccagctgg gaagcctcgg cccagggtgt cggctgcgtg ccgctcgatg
     1801 ccaccgatcc gctctacgtc ctttacacgt cgggcaccac cggaaagccc aagggtgtgg
     1861 tgcgggacaa cggcggccac gcggtggcgc tgaactacag catggaagcc atctacggca
     1921 tggacgcggg cgacgttttc tgggccgcgt cggacgtcgg ctgggtggtg ggccattcct
     1981 acatcgtcta cggacccctc atccgtggct gcacctcggt cctgttcgaa gggaagcccg
     2041 tgcggacccc cgacgcgggc gttttctggc gcgtgattca ggatcaccgg gtgaagtgct
     2101 tcttcgtcgc gccaacggcc ttgcgcgcga ttcgcaagg
//
LOCUS       DBNHQR010000446        10194 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_549473, whole genome shotgun sequence.
ACCESSION   DBNHQR010000446 DBNHQR010000000
VERSION     DBNHQR010000446.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10194)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10194)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10194
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_549473"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="AC16VR_10050"
     CDS             <1..317
                     /locus_tag="AC16VR_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HIR6011542.1"
                     /translation="HQTDAALYWPLGRRFGLIGRWNYDIESGRTIEGLAGIEYNNCCW
                     QLRLVARRYIDSPSARQIATVDADKGIFFQFVFKGLAGFGDRLEAVLRRGIRGYSTEV
                     YD"
     gene            350..1627
                     /locus_tag="AC16VR_10055"
     CDS             350..1627
                     /locus_tag="AC16VR_10055"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR6011543.1"
                     /translation="MSLTLGLLTASALASAPALAEYRELDAIVAVVDEDVVLASELLS
                     RLKSIREQFAEQQGQLPSDDILVSQLMERLIIESLQMQEAERRGVQIDDETLTRAVMS
                     FAERNGMSAEQFRQALAADGVSYVEFREQIKQEMTLQRLQRNLVNRRITITDSDIDDL
                     LNSPYYQQLLSDEFRVGHILLAIDDKATEEAIQEAARQAVEIVEELRAGADFAQIAVA
                     RSAGSRALEGGDLGWRRAGELPSLFADQVLALEPGETAEPVRSASGIHIVQLLEQRGA
                     GMQKEEQTLLRHILVMPSEIRSEEEAEALIRQVHDELEAGGDFVALAAEHSEDPGSAL
                     NGGDLGWTSGNEFVPAFREAMYATTTGELSEPFRSQYGWHVLEVQERREQDLSEETRR
                     NMAMQILHQRRFDEELQEWLKELRDEAFVEMRL"
     gene            1633..2613
                     /gene="pdxA"
                     /locus_tag="AC16VR_10060"
     CDS             1633..2613
                     /gene="pdxA"
                     /locus_tag="AC16VR_10060"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017640355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HIR6011544.1"
                     /translation="MPISLAITPGEPAGVGPELLLRLAESPRTRPWVAVADADLLAER
                     ARALGTTVRLDDAWDRPAMEAGRLTVRHVPLEAPCTPGQLDPRNAAYVLETLRLATDG
                     CLSGEFAGLVTGPVQKSVMNDAGIPFTGHTEFLCERAGVADVLMLLVAGTLRVGLATT
                     HLPLREVPDALSRALLEGRLGLLLDGLRRDFGIARPRVLVAGLNPHAGEGGHLGTEEI
                     EIIDPVCERYRAAGEQVKGPLPADTLFTPAMLDTADAVLAMYHDQGLPVLKHAGFGRA
                     VNVTLGLPFVRTSVDHGTALDLAGSGVADPGSFAAAASLAGDLLDFRASA"
     gene            2660..3376
                     /gene="rsmA"
                     /locus_tag="AC16VR_10065"
     CDS             2660..3376
                     /gene="rsmA"
                     /locus_tag="AC16VR_10065"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003206170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HIR6011545.1"
                     /translation="MADLIRLGPHDQVLEIGPGTGALTAALLDASGAAPARYLAVEID
                     RDLAPELGRRFPTVEVVNADVLRVPLDELLGSQSWRVVGNLPYNISTPLLVRLFACTD
                     RIRDMHFMLQREVALRLAAVPGTKAWGRLTVLAQYHCQVELLFEVAPESFSPPPKVWS
                     AVARLVPHAAKLPLENPDLLDLVLRHAFSGRRKRLGNALKSLSMPWHELDVDDAARPD
                     QLSVKDFVTLANAVQLEQTP"
     gene            3373..3753
                     /gene="apaG"
                     /locus_tag="AC16VR_10070"
     CDS             3373..3753
                     /gene="apaG"
                     /locus_tag="AC16VR_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HIR6011546.1"
                     /translation="MSGPASGIEVAVDARYVVGESDPIRNRYVFAYTITISNLGPEPG
                     QLLDRHWFIADGDNEVREVQGPGVVGEQPRLEPGTAFRYTSAAVLETPVGTMHGLYGF
                     RRDDGTRFEVPIPMFSLAAPNAVH"
     gene            3763..4575
                     /locus_tag="AC16VR_10075"
     CDS             3763..4575
                     /locus_tag="AC16VR_10075"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HIR6011547.1"
                     /translation="MATYAIGDVQGCYAEFCRLLKKMEFDKKRDRLWMVGDLINRGPD
                     SLRVLNLVRSLGKRAVIVLGNHELHFLAIHYGGYSANRTDTFDDLLSSPARAEIAEWL
                     CQQPLLVRSNKLGYVMTHAGIPHIWDIDVAEALAGEVEKAIRGKRRKRYFESMYGNVP
                     DVWDPKLAGMDRLRCITNYFTRMRLVDKAGRLDFSFKGGLADAPKPWLPWYELRNRKP
                     LPVKLVFGHWAALQGHTGLKDVIALDTGCVWGRKLSALCLDTGEKYSVRAGG"
     gene            4867..6789
                     /locus_tag="AC16VR_10080"
     CDS             4867..6789
                     /locus_tag="AC16VR_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HIR6011548.1"
                     /translation="MGVFEHYQERFEAAREEELSLQEYLDLCKEQPATYATAAERLLL
                     AIGEPEFVDTAKDSRLSRIFSNKIIKRYPAFDDFYGMEEAIEQIVSFFRHAAQGLEEK
                     KQILYLLGPVGGGKSSLAEKLKALMQREPFYALKGSPVNESPLGLFDPVEDGAILESD
                     YGIPRRYVRGLMSPWAAKRLKEFGGDITQFRVVKKYPSILDQVALSKTEPGDENNQDI
                     SALVGKVDIRKLEDFAQNDPDAYSFSGGLCKANQGMLEFVEMFKAPIKVLHPLLTATQ
                     EANYNGTEAIGSIPFDGIVLAHSNESEWQTFRNNRNNEAFIDRVYIVKVPYCLRVREE
                     VNIYRKLIDTSSLNAAPCAPDTLRMLAQFSVLSRLKEPENSNVFSKMRIYDGENLKDT
                     DPKAKTLQEYRDTAGVDEGMDGLSTRFAFKILSKVFNFDAIEVAANPVHLMYVLEQQI
                     EQEQYPQEVRDRYLRFIKEYLAPKYVDFIGKEIQTAYLESYSEYGQNVFDRYVTYADF
                     WIQDQEYRDPETGDILDRASLNEELEKIEKPAGISNPKDFRNEIVNFVLRARASNNGT
                     NPSWLSYEKLRTVIEKKMFSNTEDLLPVISFNAKSSSDEQRKHDDFVARMVERGYTDK
                     QVRLLAEWYLRVRKSQ"
     gene            6865..8133
                     /locus_tag="AC16VR_10085"
     CDS             6865..8133
                     /locus_tag="AC16VR_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009685232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HIR6011549.1"
                     /translation="MSYIIDRRENAKNKSAVNRQRFLERYKQRIKKAVSDAVNERSIT
                     DMERGEEVTIPAQDISEPVFQHGSGGRRSIVHPGNKEFIAGDRIARPEGGAGGGSGDG
                     EASNSGEGSDDFVFQLTQEEFLEYLFDDLALPNLTKRHLKGNSSFKYEHAGFTSDGVP
                     AKLSVLRSLRQAKARRIALAGEPKRLLREAREAFDQAELDGDELQRRRMRDEIERLEK
                     RLGRIPFLDTNDLRYNLHLRKPVPTSQAVMFCLMDVSGSMDQHIKDMAKRFYLLLYMF
                     LKRHYERTEIVFIRHHTVAKEVDEQEFFYSRETGGTVVSSALKLMLEVLEDRYSPNEW
                     NIYGAQASDGDNWNDDSPLCRQLLEKKILPLVQYFSYVEITKRSHQALWREYEHLAER
                     FPESFAQRQITDVSDIFPVFHDLFQKKDAA"
     gene            8133..9665
                     /locus_tag="AC16VR_10090"
     CDS             8133..9665
                     /locus_tag="AC16VR_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011531833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HIR6011550.1"
                     /translation="MEILLPGRGPREPISDGGDWTFELIGRYDREIGAIAEQFGLDTY
                     PNQIEIIRSDQMMDAYASIGMPIGYHHWSFGKHFVDVERSYQRGFMGLAYEIVINSNP
                     CISYLMEENSMTMQALVIAHACYGHNSFFKGNYLFQTWTDASAIVDYLLFARNYIASC
                     EERYGVQEVEMILDSCHALMNYGVDRYKRPYPISAEEEKRRQAERAEYVQRTLNDLWR
                     TLPQARQLDSSDEEVFPREPQENLLYFIEKHAPLLEPWQREIVRIVRKVAQYFYPQRQ
                     TQVMNEGWASFWHYTLLNELYDRGLVTDGFMMEFLASHTAVLFQPSFDHPAYRGINPY
                     ALGFAIYSDLRRVCEHPTDEDREWFPDIAGGNWLEVLPAAMRDFKDESFVLQFLSPRV
                     MREMRLFGVVDDDAEDEVLISAIHDRGGYREVRSLLAGQYNIGDREPNIQVVRVDVRG
                     DRSLTLRHAQHARQPMGQSTDEVMRHLHRLWGFPVRLESVWHDEVVATTACPAEEAQS
                     AA"
     gene            9823..>10194
                     /locus_tag="AC16VR_10095"
     CDS             9823..>10194
                     /locus_tag="AC16VR_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003286177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HIR6011551.1"
                     /translation="MGRLGELLAPDHNGIRLPAGFSSRIVAESGNRPVSSSSYAWHSA
                     PDGGATFAHPEGGWVYVSNCEMPLGFGGASALRFDESGTLVDAYPILGRTSGNCAGGA
                     TPWGTWLSCEEVRRGRVYECDP"
BASE COUNT         1868 a         3237 c         3312 g         1777 t
ORIGIN      
        1 ttcaccagac cgatgccgcc ctctactggc cactcggacg tcgcttcggc ctgatcggcc
       61 ggtggaacta cgacatcgag agcggtcgaa cgattgaggg cctggcggga atcgagtaca
      121 acaactgctg ctggcagctg aggctggtgg cacgacgtta catcgacagc cccagcgcgc
      181 ggcagatcgc cacggtggac gcggacaaag gcatcttctt ccagttcgtc ttcaagggcc
      241 tggcggggtt cggcgatcgg ctcgaggcgg tgctgcgccg cggcatccgg ggatacagca
      301 cggaggttta cgactgatgg ttggtttggg aagcggatcg cgacgggtga tgagcctgac
      361 gctcgggttg ctgacggcct ctgcgcttgc gagcgcgccg gcgttggccg agtaccggga
      421 actcgacgcc atcgtggcgg tggtggacga ggacgtggtg ctcgcctcgg agctgctttc
      481 gcgcctgaaa agcatccgcg agcagtttgc cgagcagcag ggtcagctgc cgtcggacga
      541 cattctggtc agccagctca tggagcggct catcatcgag agcctgcaga tgcaggaggc
      601 cgagcgccgc ggcgtgcaga tcgacgacga gaccctgacc agggcggtga tgtcgtttgc
      661 agagcgcaac gggatgagcg cggagcagtt ccgtcaggca ttggccgccg acggggtcag
      721 ctacgtggag tttcgcgagc agatcaagca ggagatgacc ctgcagcgcc tgcagcgcaa
      781 cctggtgaac cgtcgcatca ccatcaccga cagcgacatc gacgatctgc tgaactcgcc
      841 ctactaccag cagctgcttt ccgacgagtt cagggtcggg cacattctgc tggccatcga
      901 cgacaaagcg accgaggaag ccatccagga ggcggccagg caagcggtag aaatcgtcga
      961 ggagcttcgc gcgggcgcgg acttcgctca gatcgccgtg gcccggtcgg cggggagtcg
     1021 ggcgctggaa ggcggcgatc tcggctggcg gagagccgga gagctgccga gcctctttgc
     1081 cgatcaggtc ctggcactcg agcccggcga gaccgccgag cccgtccgca gcgccagcgg
     1141 catacacatc gtccagctgc tcgagcagcg gggcgcgggc atgcagaaag aagagcagac
     1201 gcttctgcgt cacatcctgg tcatgccctc ggagatccgc tcggaagagg aagccgaagc
     1261 gctcatccgc caggttcacg acgagctcga ggccggcggc gatttcgtcg ccctggcggc
     1321 cgagcactcg gaggatccgg gcagcgcgct caacggcggt gacctgggct ggacgtcggg
     1381 caacgagttc gtgcccgcgt tccgcgaggc gatgtatgcg accacgacgg gcgagctgtc
     1441 cgaaccgttt cgctcccagt acggctggca cgttctggag gttcaggagc gacgcgagca
     1501 ggatttgagc gaagagaccc gccgcaacat ggcgatgcag atcctccatc agcgccgctt
     1561 cgacgaggaa ctccaggagt ggctgaagga actgcgggac gaagcgttcg tggagatgag
     1621 gctctagcgg gtgtgccgat aagcctcgcc atcacgcccg gcgagccggc gggcgtcggc
     1681 ccggagcttc tgctccgact ggcggaaagt ccccgcacgc gcccctgggt agcggtggcc
     1741 gacgcggatc ttctggccga gcgggcccgc gccctgggca ccacagtccg gttagacgac
     1801 gcctgggacc gcccggccat ggaagccggg cggctgaccg tgcggcacgt gcctctcgag
     1861 gctccttgca cccccgggca actcgatccc cgcaacgccg cctacgtcct ggaaaccttg
     1921 cggctggcga cggacggttg cctgtcgggg gagtttgccg gccttgtcac gggacccgtg
     1981 cagaagtcgg taatgaacga cgcgggcatc ccgttcaccg ggcacacgga gttcctgtgc
     2041 gagcgggcgg gcgttgccga cgtgctcatg ctgctggtgg cgggcacgct tcgggtcggg
     2101 ctcgccacga cccatttgcc cctgcgcgag gtgcccgacg ccctttcccg agctctgctc
     2161 gagggccggc tcggcctgct gcttgacggg ctgcgaaggg atttcggtat tgcccgaccc
     2221 cgggtactgg tcgccggttt gaacccccac gcgggggagg gcgggcacct gggtaccgag
     2281 gagatcgaga tcatcgatcc cgtgtgcgag cgctaccgcg ccgccgggga gcaggtgaag
     2341 gggccgctgc ctgccgacac cctcttcacc ccggccatgc tcgacaccgc agatgcggtg
     2401 ctcgccatgt atcacgacca gggtcttccg gtgctcaagc acgccgggtt cgggcgggcg
     2461 gtcaacgtca cgctcggcct tcccttcgtg cggacgtcgg tcgaccacgg taccgcgctt
     2521 gacctggcgg gctccggggt ggctgacccg ggcagctttg ccgccgccgc ctcgctggcg
     2581 ggcgacctgc tggatttccg tgcgagcgcg taagcggttc ggccagcact ttctcaccga
     2641 cgaagcggtg ctgcagcgca tggcggatct gatccgtctc gggccgcacg accaggtgct
     2701 ggagatcggt ccgggcaccg gggcgctcac cgccgctctg cttgacgcct ccggagcggc
     2761 gccagcgcgt tacctggcgg tggagatcga ccgcgatctg gcccctgagc tcgggcgccg
     2821 cttccccacc gtggaggtcg tcaacgccga cgtgctgcgc gttccgctcg acgagctgct
     2881 gggctcgcag agctggcgcg tggtgggcaa tctgccctac aacatctcca cgccgcttct
     2941 ggttcgtctg ttcgcctgca ccgaccgaat ccgcgacatg cacttcatgc tccagcgcga
     3001 ggtggcgctg cgcctcgcgg ccgttccggg caccaaggca tggggccggc tgacggtgct
     3061 cgcccagtac cactgccagg tggagctgct cttcgaggtt gcgccggaga gtttcagccc
     3121 accgccgaaa gtctggtccg ccgtcgcgcg gctggtgccc catgcggcca agctcccgct
     3181 cgagaacccg gacctgctgg atctggttct gcgccacgcg ttctccgggc ggcgcaaacg
     3241 gctcggcaat gcactaaaat ccttgagcat gccctggcat gaactcgatg tggatgacgc
     3301 cgcccggccc gaccagctgt cggtgaagga tttcgtcacg ctcgccaacg ccgtgcagtt
     3361 ggagcagaca ccgtgagcgg acccgccagc ggcatcgagg tggcggtgga tgcgcgctac
     3421 gtcgtcggcg agtcggatcc gatccggaac cgctacgtgt tcgcctacac cattacgatc
     3481 agcaacctcg ggccggagcc cggtcagctc ctcgaccgcc actggttcat cgccgacggc
     3541 gacaacgagg tgcgcgaggt gcaggggccg ggagtggtcg gcgagcaacc ccggctcgag
     3601 cccggcaccg ccttccgtta taccagcgcc gcggttttag aaacgcccgt gggaaccatg
     3661 cacgggctct acggcttccg ccgtgacgac ggcacgcgct tcgaggtgcc gattcccatg
     3721 ttctcgctag ccgcgcccaa cgcggtgcat tgagaccggg cagtggccac ctacgcgatc
     3781 ggtgacgttc agggttgcta cgcggagttc tgccggctgc tcaagaaaat ggagttcgac
     3841 aagaagcgcg atcggctctg gatggtgggt gatctgatca accgtgggcc cgattcgttg
     3901 cgggttctga acctggtgcg ttccctgggc aagcgcgcgg tcatcgtgct cggcaaccac
     3961 gagctccatt ttctcgccat ccattacggc ggctacagcg ccaaccggac cgacacgttc
     4021 gatgatctgc tgagctcccc ggcccgcgcc gagatcgccg agtggctgtg ccagcagccg
     4081 cttctggtcc ggagtaacaa gctgggctac gtcatgaccc atgccggtat tcctcatatc
     4141 tgggacatcg atgtcgcgga agctctggcc ggcgaggtgg aaaaagccat tcgcggcaaa
     4201 cggcgcaaac gctacttcga aagcatgtac ggcaacgtgc cggacgtgtg ggatccgaag
     4261 ctcgcaggca tggaccggct gcgctgcatc accaactact tcacccgcat gcgtctggtg
     4321 gacaaggcgg gacggttgga tttttccttc aaaggggggc tggccgacgc gcccaagccc
     4381 tggctgccgt ggtacgagct gcgcaaccgc aaaccgctgc cggtcaagct ggttttcggt
     4441 cactgggcgg cgctgcaggg gcacacgggg ctgaaggacg tgatcgccct ggatacggga
     4501 tgcgtgtggg gccgcaagct cagcgccctg tgcctggaca cgggcgagaa gtattcggtt
     4561 cgggcgggcg gctgaggtga caggggcccc gggtgggccg taacaaagcc gttttcttgc
     4621 ccggtccggc aactccggcg cagcaaggat cggttcagag gcgacttgag tcccattcct
     4681 tgggtttcgc cgttccaaat gtcaacgaat cacgcgttcc gtaccgccga taaggcggaa
     4741 ttagccgctc atccattagt gattccggtc gcaggtgcgt gtgtgacacg gttcacggat
     4801 cgacgctgaa tgcatcggta gcttcagtga ggtacacgcg gatgcagcaa aggagcgcta
     4861 gcaaaaatgg gtgtattcga gcactatcag gaacgattcg aagcggctcg cgaagaagag
     4921 ctttcccttc aggaatacct ggatctctgt aaagaacaac ccgccaccta cgccacggcc
     4981 gccgagcggc tgctgctggc gatcggcgag ccggagtttg tcgacaccgc caaggattcg
     5041 cggctttcgc gcatcttttc caacaagatc atcaagcgct acccggcgtt cgacgacttc
     5101 tacggcatgg aagaagccat cgagcagatc gtgtctttct tccgccatgc ggcccaaggc
     5161 ctcgaggaga aaaagcagat tctctacctc ctcgggcccg tcggcggcgg caagagctcg
     5221 ctcgccgaaa agctcaaagc gctcatgcag cgcgaaccgt tctacgcgct gaagggctcg
     5281 ccggtgaacg agtcgcctct ggggctgttc gatcccgttg aggacggcgc catcctcgag
     5341 tccgactacg gcatcccgcg ccgctacgtt cggggactca tgtcgccatg ggcggccaag
     5401 cggctcaagg aattcggcgg cgacatcacc cagttccggg tggtgaagaa atacccgtcg
     5461 atcctggacc aggtggcctt gtcgaagacc gagcccggtg atgagaacaa ccaggacatc
     5521 tctgccctgg tgggcaaggt ggacatccgc aagctcgagg acttcgcgca gaacgacccg
     5581 gacgcgtaca gcttctccgg cgggctgtgc aaggccaatc agggcatgct cgagttcgtc
     5641 gagatgttca aggcgcccat caaggtgctg cacccgctgc tcaccgcgac ccaggaagcc
     5701 aactacaacg gcaccgaagc catcggctcc attccgttcg acgggatcgt gctggcccac
     5761 tccaacgagt cggagtggca gacgttccgt aacaaccgca acaacgaagc cttcatcgac
     5821 cgcgtgtaca tcgtgaaggt gccctactgc ctgcgggtcc gggaggaagt gaacatctac
     5881 cggaagctga tcgataccag ctctctgaac gcggcgccct gcgcccccga cacgctgcgc
     5941 atgctggcgc agttctcggt gctttcccgg ctcaaggagc cggagaactc caacgtgttc
     6001 tccaagatgc gaatctacga cggggaaaac ctcaaggata cggaccccaa ggccaagacg
     6061 cttcaggagt accgcgacac ggccggtgtc gatgagggca tggacggtct gtccacccgc
     6121 ttcgccttca agatcctctc gaaggtgttc aatttcgacg ccatcgaggt ggccgccaac
     6181 cccgttcacc tgatgtacgt gctcgagcag cagatcgagc aggagcagta tcctcaggag
     6241 gtccgggacc ggtatctgcg cttcatcaag gagtacctgg ctcccaagta cgtggacttc
     6301 atcggcaagg aaatccagac ggcgtacctc gagtcctact ccgagtacgg tcagaacgtc
     6361 ttcgaccgct acgtcaccta cgccgatttc tggattcagg atcaggagta tcgcgacccg
     6421 gaaaccggcg acattctcga tcgcgcttcc ctgaacgagg agctggagaa aatcgagaag
     6481 cccgccggca tcagcaatcc gaaagacttc cgcaacgaga tcgtcaactt cgtgctgagg
     6541 gcgcgtgcaa gcaacaacgg caccaacccg tcgtggttga gctacgagaa gctgcgcacg
     6601 gtcatagaga agaagatgtt ctccaacacc gaggatcttc tgccggtgat ctctttcaac
     6661 gcgaagtcct ccagcgacga gcagcgcaag cacgacgact tcgtcgcccg gatggtggag
     6721 cgcggttata ccgacaagca ggtgcgactg ctcgcggagt ggtacctgag agttcgcaag
     6781 tcacagtagc aacggaaccc tctcaccgtc ccggaggctg atgcccccgg gacggtgaga
     6841 ggcaccgcgc agcgggggca tggcatgagc tacatcatcg accgccggga aaacgccaag
     6901 aacaagagcg ccgtcaatcg gcagcgtttt ctggagcgtt acaagcagcg catcaagaag
     6961 gcggtatcgg atgcggtgaa cgagcgcagc atcaccgaca tggaacgggg cgaggaagtc
     7021 accatccccg cccaggacat ctccgaaccg gtctttcagc acggcagcgg cgggcgccgc
     7081 agcatcgtgc atcccggcaa caaggagttc attgccggag accgcattgc gcgtccggaa
     7141 ggcggcgccg gcggcggttc cggggatggc gaggcctcaa actctggtga gggttccgac
     7201 gacttcgtct ttcagctcac ccaggaggag ttcctcgagt acctcttcga cgacctggcc
     7261 ctgcccaacc tcaccaagcg gcatctgaaa ggcaactcga gcttcaagta cgaacatgcc
     7321 gggttcacca gcgacggggt gcccgccaag ctctcggtgc tgcgttccct gcggcaggcg
     7381 aaggcgcggc gcatcgcgct ggccggcgag cccaagcggc tgcttcgcga ggcgcgcgag
     7441 gcgttcgatc aggcggagct cgacggcgat gagctccagc gtcgccgcat gcgcgacgag
     7501 atcgagcgct tggaaaaacg gctcggccgc ataccctttc tggataccaa cgatctgcgc
     7561 tacaacctgc acttgcgcaa gccggtgccc acctcccagg ccgtgatgtt ctgcctcatg
     7621 gacgtgtccg gctccatgga ccagcacatc aaggacatgg cgaagcgctt ctacctgttg
     7681 ctctacatgt tcttgaagcg ccattacgaa cgcacggaga tcgtcttcat ccggcatcac
     7741 accgttgcca aggaggtgga cgagcaggag ttcttctatt ccagggaaac gggcgggacc
     7801 gtggtctcca gcgcgctcaa gctgatgctc gaggtgctgg aagatcggta ctcgcccaac
     7861 gagtggaaca tttacggggc ccaggcctcg gacggggaca actggaacga cgactcgccc
     7921 ctctgccggc agcttctgga gaagaagatc ctgcctctgg tgcagtactt ctcctacgtg
     7981 gagatcacca agcgcagcca tcaggcgctg tggcgggagt acgagcacct ggccgagcgg
     8041 tttccggaat cgtttgccca gcgccagatc accgacgtgt cggacatctt tcccgtgttt
     8101 cacgacctct tccagaagaa ggacgccgcg tgatggaaat cctcttgccg ggcagggggc
     8161 cgcgcgagcc catctccgac ggcggggact ggacgttcga gctcatcggt cggtacgacc
     8221 gcgagatcgg cgcgattgcc gagcagttcg gtctcgacac gtaccccaat cagatcgaga
     8281 tcatccgttc ggaccagatg atggatgcct atgcctccat cggcatgccc atcggctatc
     8341 accactggtc cttcggcaag cacttcgtcg acgtcgagcg cagctaccag cgcggattca
     8401 tgggcctggc ctacgagatc gtgatcaact cgaatccctg catttcctac ctcatggaag
     8461 agaacagcat gaccatgcag gccctggtca tcgcccatgc ctgctatggc cacaactctt
     8521 tcttcaaggg gaactacctg ttccagacct ggacggacgc cagcgccatc gtcgactacc
     8581 tgctcttcgc ccgtaactac attgccagct gcgaggagcg ctacggcgtg caggaagtgg
     8641 agatgatcct cgactcctgt cacgccctga tgaactacgg ggtcgaccgc tacaagcggc
     8701 cgtacccgat ctcggcagag gaagagaagc ggcggcaggc cgaacgcgcc gagtacgtcc
     8761 agcgcacgct caacgatctg tggcggacgc ttccccaggc ccggcagctc gactcgagcg
     8821 acgaggaagt cttcccgcgg gagcctcagg aaaatctcct ctacttcatc gagaagcacg
     8881 cgccgctctt ggagccctgg cagcgcgaga tcgtgcgcat cgtccggaag gtggcgcagt
     8941 acttctaccc tcagcggcag acccaggtga tgaacgaggg gtgggcgtcg ttctggcatt
     9001 acaccctttt gaacgagctc tacgatcgcg gcctggtcac cgacggcttc atgatggagt
     9061 ttctcgcctc gcacacagcg gttctgttcc agccgagttt tgatcacccc gcgtaccggg
     9121 gcatcaatcc ctacgcgctg ggctttgcca tctactcgga tctgcggcgc gtgtgcgagc
     9181 atccgacgga cgaggaccgc gagtggtttc ccgacatcgc cgggggcaac tggctcgagg
     9241 tgctgccggc cgccatgcgg gacttcaagg acgagagctt cgtgctgcag ttcttaagcc
     9301 ccagggtcat gcgcgagatg cgtctgttcg gcgtcgtcga cgacgacgcc gaagacgagg
     9361 tgctcatctc ggccattcac gatcggggcg gctaccgcga ggtgcgcagc cttctggccg
     9421 ggcagtacaa cattggcgac cgggagccga acattcaggt ggtgcgggtc gacgtcaggg
     9481 gtgaccggag cctcacgctg cggcacgcgc agcatgcgcg ccagcccatg ggtcaaagca
     9541 ccgacgaggt catgcggcac ctgcatcggc tctggggatt tcccgttcgc ctggagtccg
     9601 tctggcacga cgaggtggtt gccaccaccg cctgccccgc ggaggaggct cagtcggcgg
     9661 cgtagcccct gttacaatgc cgagatggac agacgatctt tcctgaccat tgccggtatc
     9721 ggcaccgcgt atctgctgag gccctcgctc gctcgcgcct gccctaccca aggtgggtcg
     9781 tcgtctgcca attgggcgga cagactgggc cctgggtccg cgatgggccg cctgggcgag
     9841 ctgcttgccc cggatcacaa cggtatccgg ctgcctgccg ggttcagctc gaggatcgtt
     9901 gccgagtccg gcaatcgccc cgtgtcgtcc tcgtcctacg cctggcactc ggccccggat
     9961 ggaggtgcta cgtttgcgca tcccgaagga ggttgggtct acgtcagcaa ctgcgagatg
    10021 ccgctggggt tcggcggtgc cagcgccctg cggttcgatg agtcgggaac cctggtagac
    10081 gcctacccga tcctcggccg aacgagcggc aactgcgctg gcggggcgac gccgtgggga
    10141 acctggttga gctgcgagga agttcgccgc ggccgggtat atgaatgcga cccg
//
LOCUS       DBNHQR010000447         2690 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_612813, whole genome shotgun sequence.
ACCESSION   DBNHQR010000447 DBNHQR010000000
VERSION     DBNHQR010000447.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_612813"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="AC16VR_10100"
     CDS             complement(<1..598)
                     /locus_tag="AC16VR_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014420717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR6011552.1"
                     /translation="MLKTFVNAGVEVCFTNPGTSEMHFVAALDTEDGMRCVLGLFEGV
                     VTGAADGYARMAGKPACTLLHLGPGLANGLANLHNARRANSPIVNVVGEHATYHRHLD
                     APLTSDIEGFASPVSGWTRVSESAASVAADGAEAVRASLQPPGQIATLILPADTAWNE
                     PAAEADPLPSADPGPFDALAVEAAARALRSGEPCALLLN"
     gene            747..2099
                     /gene="gorA"
                     /locus_tag="AC16VR_10105"
     CDS             747..2099
                     /gene="gorA"
                     /locus_tag="AC16VR_10105"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019751004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HIR6011553.1"
                     /translation="MSYDYDLFVIGAGSGGVRAARMSAGYGARVAVAESTYLGGTCVN
                     VGCVPKKLFVYGSHFHEDFEDAAAYGWDVSPTGFDWPRLRDNKTREIERLNGVYANLL
                     EKAGVETKWGHARIAGPNTVAMDGQTFTAEHILIATGSWPAVPDVPGRELAITSNEAF
                     YLESFPKRVVVVGGGYIAVEFAGIFKGLGADVDLLYRRDLFLRGFDDGVRQFVANELT
                     QKDIGLRFNTNVARIEAVDGRRRVHLVEGGSLDADLVLYATGRTPKTGDLGLETVDVA
                     LNERGGIVVDEFYRTTAPGIYALGDVIDNVQLTPVAIAEGMCVSANLFTDRPASRVNY
                     ENIPTAVFCQPNIGTVGPTETEARERYPDLAVFESEFRPMKHTLTGRQERTFMKLLVD
                     GASDRVVGAHMVGPEAGEIVQGIGVAITAGATKAHFDQTIGIHPTAAEEFVTMREPAR
                     "
     gene            2129..>2690
                     /locus_tag="AC16VR_10110"
     CDS             2129..>2690
                     /locus_tag="AC16VR_10110"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008174004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HIR6011554.1"
                     /translation="MKRMTSFRTRWPRWLAATSLTALAACATMPEQEPASRNVILFLG
                     DGMGISTVTAARIFEGQRQGLAGEEHALAFEHFPHVALVKTYNTDGQVPDSAGTMSAI
                     MTGEKTRVGHIAVGPEVDNDDCPGSLAGKRQTLLEEAELRGYATGIVSTARITHATPA
                     ATYAHVPNRNWEADSMLPAPASAAGCR"
BASE COUNT          472 a          864 c          939 g          415 t
ORIGIN      
        1 cgtttaagag cagcgcgcag ggctcgccgg agcgcaacgc ccgggcggcc gcttccacgg
       61 cgagggcatc gaatggtccg gggtcggccg aaggaagagg atcggcctcg gccgcaggtt
      121 cgttccaggc cgtgtcggcg ggcaggatga gggtggcgat ctgccccggc ggctgcagcg
      181 aagcccgcac cgcctcggcg ccatcggcgg ctaccgatgc ggctgactcc gagacgcgcg
      241 tccagccgga aaccgggctc gcgaagcctt cgatgtcgga ggtcagcggc gcgtccagat
      301 gccggtggta ggttgcgtgc tcacccacca cgttgacgat tggagagttc gcccggcggg
      361 cgttgtgcag attggcgagg ccgttggcca ggcccgggcc caggtgcagc agcgtgcatg
      421 ccggcttgcc ggccatgcgg gcgtacccgt ccgccgctcc ggtgaccact ccctcgaaca
      481 gcccgagcac gcagcgcatg ccgtcctccg tgtccagcgc ggcaacgaag tgcatctccg
      541 aggttcccgg attggtgaag cagacttcca cgcctgcgtt cacgaacgtc ttcaacaagc
      601 tctgcgcgcc gttcatggcc gtaccggtga cgagaggaaa gcggagtgta tgtgggccgg
      661 aagaagcatt gcaattcggt gctcatcaat tacgctccgt cacatcccgc atctgcgggt
      721 ccgtttcccc ggggagaatc gagacgatga gctacgacta cgacctgttc gtgatcggcg
      781 cgggatccgg cggcgtcaga gccgcgcgga tgtcggccgg atacggcgcc cgggtggccg
      841 tagccgagag cacctacctg ggcggcacgt gcgtcaacgt gggctgcgtg cccaagaagc
      901 tcttcgtcta cggctcccac tttcacgagg atttcgagga cgccgcggcg tacggctggg
      961 acgtttcgcc cacgggattc gactggccga ggctgcgcga caacaagacg cgggagatcg
     1021 agcggctcaa cggcgtgtac gcgaacctgc tcgagaaggc gggcgtggaa accaaatggg
     1081 gccatgcgcg aatcgcaggg ccgaacacgg tggccatgga cgggcagacg ttcacggccg
     1141 agcacattct gatcgccaca ggcagctggc ccgcggtgcc cgacgtcccc ggccgggagc
     1201 tggccatcac ctccaacgaa gcgttctatc tggagtcgtt tcccaaacgc gtggtggtgg
     1261 tcggcggcgg ctacatcgcg gtggagtttg ccggaatatt caaaggcctc ggcgcggacg
     1321 tagacctgct ctaccgtcgc gatctcttcc tgcggggctt cgacgacggg gtgcgtcagt
     1381 tcgtcgccaa cgagctcacc cagaaggaca ttggtctgag gttcaacacc aacgtggccc
     1441 gcatcgaagc ggtggacggc cggcggcgcg tgcacctggt ggagggtgga tctctcgacg
     1501 cggatctggt gctctacgct accggcagga ccccgaagac gggcgatctg ggcctggaaa
     1561 cggtagatgt tgcgctcaac gagcgtggcg gcatcgtggt ggacgagttc taccggacga
     1621 cggcgcccgg catctatgcg cttggcgacg tgatcgacaa cgtgcagctg acgccggtag
     1681 ccatcgccga ggggatgtgc gtgtctgcca acctgtttac cgaccggccg gccagccgcg
     1741 tgaattacga gaacattccc accgccgtgt tctgccagcc gaacatcggc acggtggggc
     1801 cgacggagac cgaagcccgg gagcgctacc cggaccttgc cgtgttcgag tcggagttcc
     1861 ggcccatgaa gcacacgctg accggccggc aggagcgcac gttcatgaag cttctggtcg
     1921 acggtgcgag tgaccgcgtc gtcggtgcgc acatggtcgg tccggaagct ggcgagatcg
     1981 tccagggcat cggcgtcgcc atcacggcgg gtgcgaccaa ggcgcacttc gaccagacca
     2041 tcggcattca ccccacggcg gcggaggagt tcgtcaccat gcgcgagccc gcaaggtagc
     2101 cgcgggtgca gcaaggggag cggacggcat gaagaggatg acaagtttcc ggacgcgatg
     2161 gccgagatgg ctcgctgcca catccctcac ggcgctggcg gcctgcgcga ccatgccgga
     2221 gcaggaaccg gccagccgga acgtcattct cttcctcggc gacggcatgg gcatcagcac
     2281 cgtcaccgcg gcccgcatct tcgaggggca acgtcagggc ctcgcgggcg aggagcacgc
     2341 gctggctttc gagcactttc ctcacgtggc gctggtgaag acctacaaca cggacgggca
     2401 ggttccggac agcgccggga ccatgtccgc gatcatgacc ggggagaaga cccgcgtagg
     2461 ccacatcgcc gtggggccgg aggtcgacaa cgacgattgc ccgggatctc tggccggcaa
     2521 acgtcagacc ctattggaag aggccgagct gcgcggctac gcgacgggca tcgtctccac
     2581 ggccaggatc acccacgcga cgccggcggc cacctatgcc cacgtgccca accggaactg
     2641 ggaagccgac agcatgctgc ccgccccggc ttccgcggcc ggctgcaggg
//
LOCUS       DBNHQR010000448         4812 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_992841, whole genome shotgun sequence.
ACCESSION   DBNHQR010000448 DBNHQR010000000
VERSION     DBNHQR010000448.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4812)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4812)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4812
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_992841"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="AC16VR_10115"
     CDS             complement(<1..533)
                     /locus_tag="AC16VR_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008569903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HIR6011555.1"
                     /translation="MNTAEIRSAFLSFFAEREHRVVPSSSLVPANDPTLLFTNAGMNQ
                     FKDALLGREDLGYRRAATAQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     EETIGWAWDLVTDVFAVPVEKLWVTVHTSDDESRAIWERKIGVPAERVISLEENFWAM
                     GDTGPCGPCSELFYDHGP"
     gene            complement(651..1139)
                     /locus_tag="AC16VR_10120"
     CDS             complement(651..1139)
                     /locus_tag="AC16VR_10120"
                     /inference="COORDINATES: protein motif:HMM:NF046475.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HIR6011556.1"
                     /translation="MLIEHKAVQEPDSAGDEQFQVAFARGLKLLATREHSAAELRRKL
                     RVKGFSEALAGDAVAALQDQGLQSDRRFAEVFVRSRLSKGYGPVRIRRDLYQRGIGDD
                     VIDEELTFPAERWVELATAVRAKRFPESEPTEHGVWNVQARFLSRKGFPADIIYRVLG
                     QV"
     gene            complement(1172..2260)
                     /gene="recA"
                     /locus_tag="AC16VR_10125"
     CDS             complement(1172..2260)
                     /gene="recA"
                     /locus_tag="AC16VR_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019342350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HIR6011557.1"
                     /translation="MANEQSNRKDSQADSQQDPQRSRALSAALAQIERQFGKGSMMRL
                     GDRELVAIPAIPTGSLGLDIALGIGGLPRGRVVEIYGPESSGKTTLTLQVIAEAQKGG
                     GTCAFIDAEHALDPIYADALGVDTDNLLVSQPDTGEQALEICDMIVRSGAVDVVVIDS
                     VAALTPKAEIEGEMGDAHVGLQARLMSQALRKMTGNIKQSNTLVIFINQIRMKIGVMF
                     GSPETTTGGNALKFYSSVRLDIRRIGAVKEGEEVVGNETRVKVVKNKVAPPFRQVEFQ
                     ILYGEGINHLGEVLELGVQQGIVEKSGAWYAYKSDRIGQGRKNACDYLESNPEIAAEI
                     EAQIREQLLPVRERSEDLVIPEPAQETL"
     gene            2394..>4812
                     /gene="mutS"
                     /locus_tag="AC16VR_10130"
     CDS             2394..>4812
                     /gene="mutS"
                     /locus_tag="AC16VR_10130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HIR6011558.1"
                     /translation="MNTGSRTSHTPMIQQYLSIKARHPDDLLFYRMGDFYELFYEDAE
                     RAAELLDITLTARGQSAGKPIPMCGVPYHAADGYLARLVRQGVRVAICEQIGDPATSK
                     GPVAREVQRIVTPGTLTEEALQDARRDSILMAINPGPDEVYGLALLNLAAGRLQVAEV
                     RTRPLLAAEVARSDPSEILAVDELPEAVTGQRHLTRRDPLEFDTDLGRAKLTRHFATR
                     SLEGFDVPEDSPMVGAAAAVLEYAKSSQCRDLAFIDAMHRVAADDVVVLDPHSRRNLE
                     IDRRVDGSEENTLFALFNTTQTPMGARLLRSWLAGPSRNAHTVEARQASVESLLGADN
                     DNLRRLLKEVGDLERIVARVALGSATPRDLSRLRTGLGQVPDVKDFLQTLEAPRLADL
                     MARLPDFNDEVELLGTAIVEAPPATIRDGGVIASGYDAQLDSLRNLSDNAAGWLADLE
                     LRERERTGIATLKVGYNRVHGYYIETSRAAGNEVPAEYVRRQTLKNAERYITPELKQF
                     EDEALTSQARALKLEKQLYQNLLEQLGARAGGLRDAAAILAELDVLSCFAERASALRL
                     VRPEFSADPIIDIRQGRHPLVEQASAKPFVANDLSLGEKARMLIVTGPNMGGKSTYMR
                     QSALIVLLAFAGSYVPAEAARLGPIDRIFTRIGAADDLAGGRSTFMVEMTETANILRN
                     ATPRSLVLLDEIGRGTSTYDGLALAWATAEHLARETGAFTLFATHYFELTELPEELSQ
                     CRNVHLTAAEHDGKIVFLHSVEPGPASQSYGVQVARLAGVPNEVIARARTRLQSLEQQ
                     QAERIAQQ"
BASE COUNT          896 a         1613 c         1476 g          827 t
ORIGIN      
        1 ggcccgtgat cgtaaaacag ctcgctgcaa ggcccgcagg gtcccgtgtc acccatggcc
       61 cagaagttct cttccagcga gatgacccgc tccgcgggaa cgccgatttt ccgctcccag
      121 atggccctcg actcgtcatc cgaggtgtga acggtcaccc acagcttctc tacgggaaca
      181 gcaaatacgt ccgtgacgag atcccacgcc cagccgatgg tctcttcctt gaagtagtcg
      241 ccaaaactga agtttccgag catctcgaaa aacgtgtggt ggcgagccgt gtatccgacg
      301 ttttccagat cgttgtgctt accacccgcc ctgacgcaac gctgagccgt cgccgcgcga
      361 cgatagccta gatcctcgcg accgagcagc gcgtccttga actggttcat tccggcgttg
      421 gtgaaaagca aggtcggatc gttggccggc accagggaac tcgacggcac gacgcggtgc
      481 tcccgctcgg cgaagaacga caggaatgct gaacggattt cagcggtgtt catctttgga
      541 atcaaccaat taggaggcgc gcagtatacg gaaaccgcgt ccaattgaca tggccgcctg
      601 gtctgccacg gggtgacgga cagggcctcc gcggccgcga gattgggcgc ctagacctgc
      661 cccagcaccc ggtaaatgat gtcggccgga aaccccttgc gggacagaaa acgcgcctgc
      721 acattccaga cgccgtgctc tgtgggctcg ctttccggaa accgtttggc ccgaaccgca
      781 gtggcgagtt cgacccagcg ttccgccggg aaggtcagct cctcgtcaat cacgtcatcg
      841 ccgatgccgc gttgatatag atcccgacga attcggacgg gcccgtaccc cttgctgaga
      901 cgactgcgaa caaacacctc cgcgaagcgc cggtcggatt gcagcccctg gtcctgcagc
      961 gcagcgacgg cgtctccggc caacgcctcg gaaaatcctt tgacgcgaag cttgcgtcga
     1021 agctcggccg ccgaatgctc ccgggtggcg agcagcttga ggcctcgggc aaacgccacc
     1081 tggaactgct catcgcccgc gctgtccggc tcctgaaccg ccttgtgctc aatcaacatg
     1141 cgaccaccgc gctccccggg gcctgattcg tctacagcgt ttcctgggcc ggctcgggaa
     1201 tgacgagatc ctcggaacgc tctctaacgg gcaggagctg ctcgcgaatc tgggcttcga
     1261 tctcggccgc gatttcagga ttggactcga ggtagtcgca ggcgttcttc cgtccctgcc
     1321 cgatccgatc ggacttgtag gcataccagg cacccgattt ctcgacgatg ccctgctgca
     1381 cacccaactc caggacctca ccgaggtgat tgatgccctc cccgtaaagg atctggaact
     1441 cgacctgcct gaagggcgga gccaccttgt ttttgaccac tttgacccga gtctcgttgc
     1501 cgaccacctc ttcgccttcc ttcacggcgc cgatccggcg gatgtccagc cgcaccgacg
     1561 agtaaaattt cagcgcgttc ccgccggtgg tggtttccgg cgaaccgaac atgaccccga
     1621 tcttcatacg aatctgattg atgaagatga ccagggtgtt cgactgcttg atgttgcccg
     1681 tcatctttcg aagcgcctgg ctcatcagac gagcctgcag gccgacgtgg gcatccccca
     1741 tttccccttc gatctccgcc ttgggggtca gtgccgccac ggagtcgatg accaccacgt
     1801 ccacggctcc ggaacggacg atcatgtcgc agatttccag agcctgctcg ccggtatccg
     1861 gctgggatac cagcagattg tccgtatcga caccgagcgc atcggcgtag atggggtcga
     1921 gggcgtgctc cgcgtcgatg aaagcgcagg tgccgccgcc cttttgagcc tcggcaatga
     1981 cctggagggt gagcgtcgtc ttacctgacg actccggacc gtagatctcg accacccggc
     2041 cccttggcag accgccgata cccagagcaa tgtctagccc cagcgagccc gttgggatcg
     2101 ccggtatggc gaccagctcc cgatctccaa gccgcatcat cgagcctttg ccgaactggc
     2161 gctcgatctg cgccagcgcc gcggacagcg cccgggatct ctgcggatcc tgctgggaat
     2221 ccgcctgcga atctttgcgg ttggactgct cgttggccat gaatctctcc tgcggggtta
     2281 acggacggta ctgtatatat aatcagtatt gatggcaacc gctcgaaggg cccttctata
     2341 cgttctcgca catgctcttg agacatctcg gagccacaga gaataatagg ttcatgaaca
     2401 cgggatccag aaccagccac acgccgatga tccagcagta cctgagcatc aaggcccggc
     2461 atccggacga cctgctgttc taccgcatgg gcgatttcta cgagcttttc tacgaggatg
     2521 ccgagcgcgc ggccgagctg ctggacatca cgctcactgc ccgtggccag tcggcgggca
     2581 agccgatacc catgtgtggc gtgccctacc acgcggctga cgggtacctc gcccggttgg
     2641 tgcgccaggg cgtgcgggtg gccatctgcg agcagatcgg cgacccggcc accagcaagg
     2701 gaccggtggc cagagaggtc cagagaatag tcacccccgg caccctcacc gaggaggctc
     2761 tccaggacgc ccgacgcgac agcatcctca tggccatcaa tccgggcccg gacgaggtct
     2821 acggcctggc gctgttgaac cttgcggccg gcaggctgca ggtcgcagag gtgcggaccc
     2881 gcccgctgct ggcagccgag gtggcccgca gcgatccgag cgagatcttg gccgtcgacg
     2941 agttgcccga agcggtcacc ggacagcgcc acctgacgcg gcgcgatccg ctggagttcg
     3001 acaccgatct cggccgggcc aagctgacca gacacttcgc gacgcgctcc ctggagggct
     3061 tcgacgtccc cgaggactcg cccatggtgg gcgccgcggc cgccgtgctc gagtacgcaa
     3121 agtcctcgca gtgtcgcgat ctggcgttca tcgacgccat gcatcgcgtg gctgcggacg
     3181 atgtggtcgt gctggatccg cactcacggc gcaatctgga aatcgatcgg cgcgtggacg
     3241 gcagcgagga gaacacgctt ttcgctctgt tcaacacgac gcaaacgccc atgggagcac
     3301 gcctgctgcg aagctggctc gccgggccca gccgcaacgc ccacaccgtc gaagcgcgcc
     3361 aggcctcggt cgaaagcctg ctgggtgcgg acaacgacaa cctgcgccga ctgctcaagg
     3421 aggtcggcga cctggagcga atcgtcgccc gggtggcgct gggttcggcg acgccgcgcg
     3481 acctgtcacg gctgcgcacg ggcctgggac aggtgccgga cgtgaaagac ttcctgcaga
     3541 cgctcgaagc cccccggctc gccgatctga tggctcgcct gcccgacttc aatgacgagg
     3601 tcgagctgct cgggaccgcg atcgtcgagg cgcctccggc cacgattcga gatggcggcg
     3661 tgatcgcctc cggctacgac gcccaactcg atagcctgcg gaatttgagt gacaacgccg
     3721 ccggctggct ggccgatctc gagctgcgcg agcgggagcg gacgggcatc gcgaccctga
     3781 aggtcggcta caaccgggtc cacggctact acatcgagac gagccgcgcg gccggcaacg
     3841 aggttccggc cgagtacgtc cgcagacaga ccctgaaaaa cgccgagcgc tacattaccc
     3901 cggagctcaa acagttcgag gacgaagcgc tcaccagcca ggcacgagcg ctcaagcttg
     3961 aaaagcagct gtaccagaac ctgctggagc agctgggcgc aagagccggc ggtttgcgcg
     4021 atgccgcggc catcctcgcg gagctggacg tgctctcgtg cttcgccgag cgcgcaagcg
     4081 ccctgaggct ggtgcgaccc gagttctcgg cggaccccat catcgacatc aggcagggcc
     4141 gccatccgct cgtcgagcag gcgagcgcaa agcccttcgt agccaacgac ctctcgctgg
     4201 gtgagaaggc acgcatgctc atcgtgacgg ggcccaacat gggcggcaag tctacgtaca
     4261 tgaggcaatc ggccctcatc gtgctgctcg ctttcgccgg cagctacgtc ccggcagaag
     4321 ccgcgcggct aggccccatc gaccgcatct tcacccgcat cggcgcggcc gacgacctcg
     4381 ccggcggtcg ctctaccttc atggtggaga tgaccgaaac cgcgaacatt ctgagaaacg
     4441 ccacgccgcg cagcctcgtc ctgctcgacg agatcggtcg cgggaccagc acgtatgacg
     4501 ggctggcact ggcctgggca accgccgagc atctggcccg ggaaacgggc gcgttcaccc
     4561 tgttcgcaac gcactacttc gagctgacgg agctgcccga ggagctttcc cagtgccgca
     4621 acgttcatct gaccgctgct gagcacgacg ggaagatcgt cttcctgcac agcgtcgagc
     4681 cagggcccgc cagccagagc tacggtgtgc aggtggcccg acttgccggc gtgcccaacg
     4741 aggtcatcgc ccgcgcgcga actcgcctgc agtcgctgga gcaacagcag gcagagcgta
     4801 tcgcgcagca ac
//
LOCUS       DBNHQR010000449         2593 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_613082, whole genome shotgun sequence.
ACCESSION   DBNHQR010000449 DBNHQR010000000
VERSION     DBNHQR010000449.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_613082"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="AC16VR_10135"
     CDS             <1..800
                     /locus_tag="AC16VR_10135"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HIR6011559.1"
                     /translation="YLTDIVALAVSRGIEVLGLKAERPEEVLGVNDRNQLAVLERRYQ
                     LERAGELMAAGATLADPSRLDVRGAVTVGADCFIDVNVVFEGTVVMGREVSIGPGSVI
                     RDSQLGDGVRVEAHTLVDGARVEAGCSLGPFARIRPGTQLGEGVKIGNFVETKKAVLG
                     AGSKASHLTYLGDAILGANCNVGAGTVTCNYDGVDKHRTTIGDGVFVGTNSTLVAPVT
                     LGDDAFVAAGSTITGDVEKGELAVGRGRQRNVQGWVRPGRRDRADED"
     gene            816..>2593
                     /gene="glmS"
                     /locus_tag="AC16VR_10140"
     CDS             816..>2593
                     /gene="glmS"
                     /locus_tag="AC16VR_10140"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020190289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 -
                     L-glutamine:D-fructose-6-phosphate transaminase
                     (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HIR6011560.1"
                     /translation="MCGIVGAVADRNVAPILLEGLQRLEYRGYDSAGLAVIDGAGEMD
                     CRRRVGKVKELAGDLSARRCDGHMGIAHTRWATHGVPSENNAHPHVSNGRIALVHNGI
                     IENFEELREELQSEGYAFASETDSEVIVHLVDSYFDKGLSLLEAVRSAVKRLEGAYAI
                     GVIALADPDRIVAARMGSPLVVGKGLGENFIASDPLALRPVTDRFVFLEEGDLVEITR
                     DEISIWNVENESVIRASVRVELGHDDVDRGNYRHFMQKEIYQQPEVLAATLEGRIGRT
                     RVLEPAFGVQAGQIFDKVQAVTIVACGTSYYAGSIARYWIEELARIPCQVEIASEFRY
                     RQVAVTPGNLFVTISQSGETAETLAALRVAKELGYVSTLTICNVATSTLVRESDIALM
                     TQAGTEVGVASTKAFTTQLADLLLLTILIARRHGMDAAREQELVEALAGLPNAVEQVL
                     GLDGRIRVLAERFMDRSNALFLGRGPMFPVAMEGALKLKEISYIHAEGYPAGELKHGP
                     LALVDENMPVVAVAPNDELLEKVQSNLQEVRARGGALYVFADERTGFKEEPGVTVIPL
                     PPVHDTLAPIVYVVPLQLLAYHVAVL"
BASE COUNT          458 a          804 c          898 g          433 t
ORIGIN      
        1 actacctcac cgacatcgtg gccctcgcgg tcagccgcgg tatcgaggtg ctgggtctga
       61 aggcggagcg gcccgaagag gtgctgggag tgaatgaccg aaaccagctg gccgtcctcg
      121 agcggcgcta ccagctcgag cgggccggcg agctgatggc cgccggggcc accctggcgg
      181 atccgtcgcg actggacgtg cgcggcgcgg tcaccgtggg cgccgactgc ttcatcgacg
      241 tcaacgtcgt tttcgagggc accgtggtca tgggccggga agtgagcatc ggtcccggct
      301 cggtcattcg ggattctcag ctgggcgacg gtgtgcgcgt cgaagcccac accctggtgg
      361 acggtgccag ggtcgaagcc ggttgcagcc tggggccctt tgcccggatc cgaccgggca
      421 cgcagctggg cgaaggcgtg aagatcggca acttcgtcga gaccaagaag gccgtgctgg
      481 gtgcaggaag caaggccagt cacctgacct atcttggtga tgccatcctc ggggccaact
      541 gcaacgtggg cgccggcacg gtgacctgca actacgacgg tgtcgacaag caccgaacga
      601 ccattggcga cggcgtcttc gtaggcacca acagcacgct ggtcgccccg gtcacgctgg
      661 gcgacgatgc tttcgtggcg gcggggtcca cgatcaccgg cgatgtcgag aagggcgagc
      721 tggcagtggg gcggggccga cagcgtaacg ttcagggctg ggtacgacct ggccggcgcg
      781 accgcgcgga cgaggattag catgaggagc ctggcatgtg cgggatcgta ggggccgtcg
      841 ccgaccgcaa cgtggcgccc atcctcttgg aaggacttca gcggctggag tatcgcgggt
      901 acgactccgc tggccttgcc gtgatcgacg gggcgggtga gatggattgc cggcgtcgcg
      961 tcggcaaggt caaggagctc gccggagacc tgagcgcccg gcgatgcgat ggtcacatgg
     1021 gaattgccca cacgcgttgg gcaacccacg gcgttcccag cgagaacaac gcccaccccc
     1081 acgtctccaa cggccgcatc gcgctggtgc acaacggcat catcgaaaac ttcgaggagc
     1141 tgcgcgaaga gcttcaaagc gagggttatg cgtttgcgtc cgagaccgac tcggaggtga
     1201 tcgttcacct ggtggattca tatttcgaca agggattgag cctgctcgag gccgtcaggt
     1261 ccgcggtcaa gcggctggaa ggcgcttacg ccatcggcgt catcgcgctc gccgaccctg
     1321 atcggatcgt cgccgcgcgc atgggcagcc cgctggtggt tggcaagggg ctgggagaaa
     1381 acttcatcgc ttccgacccc cttgccttga gaccggtgac cgaccgattc gtgttcctgg
     1441 aggaagggga tctcgtcgag attacccgag atgagatctc catctggaac gtggagaacg
     1501 agtcggtgat ccgcgccagc gtccgggtag agctcggtca cgacgacgtg gaccggggca
     1561 attaccggca cttcatgcag aaggagatct accagcagcc ggaagtgctc gcagcgactc
     1621 tggaagggcg cataggccgt acccgggtgc tcgagcccgc attcggggtg caggccgggc
     1681 agatcttcga caaagtccag gcggtgacca tcgtggcctg cgggacgagc tactacgcgg
     1741 gctccatcgc gcgttactgg atcgaggagc tcgcgcgtat cccctgccag gtggagatcg
     1801 ccagcgaatt ccgctaccgg caggtggcgg ttaccccggg caacctgttc gtgaccatct
     1861 cccagtcggg tgagaccgcg gagaccctgg cagcgctgcg cgtggccaaa gagctcggct
     1921 acgtctcgac gctcaccatc tgcaacgtcg ccaccagcac gctggtgcgc gagtccgaca
     1981 tcgcgctgat gacccaggcc gggacagagg tgggcgtggc gtcaaccaag gcgttcacca
     2041 cgcagctggc cgacctgttg ctgctgacca ttctcattgc ccgccggcac gggatggacg
     2101 cggccagaga gcaggagctg gtggaggcgc tcgccgggct tcccaatgcc gttgagcagg
     2161 tgctcggcct cgacgggcgc atccgcgttc tggcggaacg cttcatggac cggagcaacg
     2221 cgctgtttct cggacgcggg cccatgttcc cggtggcaat ggaaggcgcg ctgaagctga
     2281 aggaaatctc ctacatccat gccgagggct atcccgccgg ggagctgaag cacggccctc
     2341 tggcgcttgt cgacgagaac atgccggtcg ttgccgtggc gccgaacgac gagctgctcg
     2401 agaaggtgca gtccaacctg caggaagtgc gcgcgcgcgg cggtgccctc tatgtctttg
     2461 ccgatgagcg caccggcttc aaagaagagc ccggggtcac ggtgatcccg ctgccccccg
     2521 ttcacgacac gctggctccc atcgtctacg tcgtgccatt acaattgctc gcctatcacg
     2581 tcgcggtgct caa
//
LOCUS       DBNHQR010000450         4020 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1626136, whole genome shotgun sequence.
ACCESSION   DBNHQR010000450 DBNHQR010000000
VERSION     DBNHQR010000450.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1626136"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..570)
                     /locus_tag="AC16VR_10145"
     CDS             complement(82..570)
                     /locus_tag="AC16VR_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018405356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HIR6011561.1"
                     /translation="MFYDGRFAAVATTMQIWVDADACPGPIKEILFRAADKRGVQLTL
                     VANHPMRVQKSAFIRLLQVEQGFDKADAFIADRVEAGDLVITADIPLAADVVEGGGAA
                     LNPRGTLYTEENIREHLARRNFMEELRSTGQISGGPAALGKPDVQKFANSLDRYLTRA
                     VG"
     gene            complement(603..1283)
                     /locus_tag="AC16VR_10150"
     CDS             complement(603..1283)
                     /locus_tag="AC16VR_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011562.1"
                     /translation="MRPWRGTCGWCIGLVGVIAAAAFPAGAVQAAAISIAEARAAEPG
                     TLVTVEGSVTVPSGRFSASTFDQGFAIQDGSAGIYVSVADDPKLNNHRRVRVTGVVGD
                     DGYGQLVLRPGVDADVEILQGAARVAARQVATGDVGEATEGLLVEVTGSVVRGPTDDL
                     PYGYSVYIDDGSGETQIFIPASTGINPFRIGYLDEGVAIRVTGLSSQFQNNLEVLPRT
                     GGDIQRGR"
     gene            complement(1280..2338)
                     /locus_tag="AC16VR_10155"
     CDS             complement(1280..2338)
                     /locus_tag="AC16VR_10155"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6011563.1"
                     /translation="MDYGIALATTMDSWRAARRAEELGFSHAWFYDTQLLNPDVFMGM
                     TLAAVNTSRIRLGTGVLIPSNRIEPVTANALVSLNKLAPGRIDFGVGTGFTGRRTMGL
                     KAIPLERMKTYIERVQALVKGETVTWDFEGETHDIAFLNPELGLINVDDPIALHVSAF
                     GPRSRDLTAELGAGWLNFGVSEPVAFGSLAKMRESWARAGRDAGDLYSTLFSLGCVLK
                     PGEAFDSPRAKAQAGTAVAVFLHDMAETSEPGSLEPVMGKAVSHVVEAYREIYLGYPE
                     GQRHLLNHRGHLMFLKPEEQDLITAELIQAITFTGERAALRERLDAFEAAGYGQFTVQ
                     LIEGHEDAIEDWAEVFFG"
     gene            complement(2352..3557)
                     /locus_tag="AC16VR_10160"
     CDS             complement(2352..3557)
                     /locus_tag="AC16VR_10160"
                     /inference="COORDINATES: protein motif:HMM:NF013188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR6011564.1"
                     /translation="MNTLLIVGAVFLIGLAADVAGRLTPLPRVTLLLLAGVLVGPSVF
                     ALIPSGFIDQWFPTLTHMALAMVGFLMGHQLSVAELRKRGKVVSLLALGKVVGAALVV
                     FVAVWALTGDLALALVLGGVATATAPAATFDVVHEAHARGAFADNLVSIVAIDDAWGL
                     VLFSVLLALAASMANGDALDASIVGAGVMEVLGSLVLGVVIGVPMAYLTGRLTFGERQ
                     GEPIQAESFGFVLACAGGAVLLDLSPILASMAMGSVVASLARHHARPFHAIEGVEWPF
                     MILFFVLAGASLEVEGVSLVGWLALAYLVARALGTHLGIYLTAGIIGTDGPTRRWMGL
                     ALMPQAGVALGMALIASQRLPGHAETILTLVLSTTILLELVSPIITRRILERVGAGGE
                     LPDATDTTN"
     gene            complement(3554..>4020)
                     /locus_tag="AC16VR_10165"
     CDS             complement(3554..>4020)
                     /locus_tag="AC16VR_10165"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6011565.1"
                     /translation="QWSQLVAVMGLVGAGLALAAGPLIDRFGAKRMLVLTISLVAVHA
                     FLVAQTQHLWQDTTYVRVMLSIWVTMDPVIMVCVIALAMAICSRENSATQFAIYMSAA
                     NLGASLGAKAYGLVAEQSTYVENYTLLGVFILVMLFTVVFHRHPLNRQSEPA"
BASE COUNT          724 a         1377 c         1324 g          595 t
ORIGIN      
        1 tccgctgtcg gggaccaagt tttccgtccg ctacacacgt ctgaacctca acccgtcaac
       61 catttggtgg cttacgccga cctaacccac cgccctggtc agataccgat ccagcgagtt
      121 cgcgaacttc tgcacgtcgg gcttgcccag cgcagccggg ccgcctgaga tctgtccggt
      181 ggagcgcagc tcctccatga agttgcgccg ggcgaggtgc tcgcggatgt tttcctcggt
      241 gtacagggtg ccgcgggggt tcagcgccgc gccgccgcct tcaaccacat cggccgccag
      301 ggggatgtcc gcggtgatca cgaggtcgcc cgcttcgacc cggtcggcga tgaaggcgtc
      361 cgccttgtcg aacccctgct ccacctgcag cagccggatg aaggccgact tctgcacccg
      421 catggggtgg ttggccacca gggtgagctg caccccgcgc ttgtccgcag cgcggaagag
      481 gatctccttg atgggcccgg ggcaggcgtc ggcatccacc cagatctgca tggtcgtggc
      541 aacggcggca aagcggccat catagaacat ccatagaaca tcgaggaccg gtggcgttgg
      601 ccttatctgc cccgttgaat atctccaccg gtgcgcggca gcacttccag attgttctga
      661 aactggctgc tcagccccgt gacgcgaatt gccacgccct catccaggta gccgatcctg
      721 aacgggttta tccccgtgct ggcgggtatg aagatctgcg tctccccgga gccatcgtcg
      781 atgtaaacgc tgtagccgta gggcagatcg tccgtcggcc cccgcacgac ggatcccgtc
      841 acctccacca gcagaccctc cgtggcctcg cccacgtccc ccgttgccac ctgccgggcg
      901 gccacgcggg cagcgccctg gaggatctcc acatccgcat cgacacccgg ccgcagcacc
      961 agctgcccgt agccgtcgtc cccgacgacg ccggtgaccc gcacgcggcg atgattgttc
     1021 agcttggggt catcggcaac ggagacgtag atccccgccg agccgtcctg gatggcgaaa
     1081 ccctgatcga acgtagacgc ggaaaaccgg ccggacggga ccgtcaccga gccctccacg
     1141 gtcaccaggg tgcccggctc cgcggcccgc gcctcggcga tggagatcgc ggcggcctga
     1201 acggcgcctg cggggaaagc ggcagccgca atgacgccga caagcccgat gcaccagccg
     1261 caggtgcccc gccacggcct catccgaaga acacctccgc ccagtcctcg atggcgtcct
     1321 cgtggccttc gatgagctgg acggtgaact ggccgtagcc ggcggcctca aacgcgtcga
     1381 gccgctcgcg cagggccgcc cgctcgccgg tgaaggtgat ggcctggatg agctcggcgg
     1441 tgatgaggtc ctgctcctcg ggcttcaaga acatcaggtg acccctgtgg ttcagaagat
     1501 gacgctggcc ctccgggtag ccgagataga tctcccgata agcctccacc acgtggctca
     1561 cggccttgcc catgacgggc tctaaagaac ccggctcgga ggtctccgcc atgtcgtgga
     1621 ggaacacggc caccgcggtg cccgcctggg ccttggccct tggcgagtcg aacgcctcgc
     1681 cgggcttcag cacgcagccc agcgaaaaca gcgtgctgta gagatcgccg gcatcccgcc
     1741 ccgcgcgggc ccaggattcg cgcatcttcg ccagcgaccc gaacgccacc ggctcgctca
     1801 caccgaagtt cagccacccg gcgccaagct ctgccgtcag gtcacgggag cgcgggccga
     1861 aggctgagac gtgcagcgcg atgggatcgt ccacgttgat gaggccgagc tcgggattga
     1921 gaaaggcgat gtcgtgggtt tcgccctcga agtcccaggt caccgtctcg cctttgacaa
     1981 gcgcctgaac gcgctcgatg tacgtcttca tccgctcgag cgggatggcc ttcaggccca
     2041 tggtgcgccg gccggtgaag ccggtgccaa cgccaaagtc gatgcgcccg ggggcaagct
     2101 tgttgaggct cacgagcgcg ttggcggtga ccggctcgat gcggttgctc gggatcagca
     2161 ccccggtgcc gaggcggatg cgtgaggtgt tcaccgccgc cagcgtcatg cccatgaaga
     2221 cgtccgggtt caagagctgg gtgtcgtaga accaggcgtg gctgaagccg agctcctccg
     2281 cgcgccgggc cgcccgccag gagtccatgg tggtcgctag agcaattccg tagtccatgt
     2341 ttgcgtccgt ttcagtttgt cgtatcggtt gcgtccggca gctcgccacc cgcaccgacg
     2401 cgttccagga tccggcgcgt gatgatcggc gagaccagct ccagcaggat ggtggtgctc
     2461 agcaccaggg tgagaatggt ctcggcatgg ccgggaaggc gctgagaggc gatgagcgcc
     2521 atgccgagcg ccaccccggc ctggggcatg agggccagac ccatccagcg gcgggtcggg
     2581 ccgtccgtgc cgatgatgcc tgcggtaagg tagataccga ggtgcgtgcc caacgcgcgg
     2641 gccaccaggt acgcgagggc gagccagcca accaggctga cgccttccac ctccagcgag
     2701 gcgccggcca gcacgaaaaa aaggatcatg aaaggccatt ccaccccctc gatggcgtga
     2761 aaagggcgcg cgtggtgcct ggcgaggctc gccaccacgc tgcccatggc catggaggca
     2821 aggatgggcg aaaggtcgag cagcaccgcg ccccccgcgc aggccagcac gaacccgaaa
     2881 gattccgcct gaatgggctc gccctgccgc tcgccgaaag tcagccgccc ggtgaggtag
     2941 gccatgggca cgccgatgac gacgccgagg accaggctcc ccagcacctc catcaccccg
     3001 gcgccaacaa tggaggcatc cagcgcgtcg ccattggcca tgctggccgc cagcgccagc
     3061 agcacggaga acaggacgag gccccaggca tcgtcgatgg cgacgatgct cacgaggttg
     3121 tcggcaaacg ctcctctggc atgagcctcg tgcaccacgt cgaaggtggc ggcaggcgcg
     3181 gtggccgtcg ccaccccgcc gagcaccagg gctagggcaa ggtctccggt cagggcccac
     3241 acggcgacga acaccaccag cgcggccccc accaccttgc ccagcgccag cagcgagacc
     3301 accttgccgc gcttgcgcag ctctgcgacg gatagctgat ggcccatgag aaagcccacc
     3361 atggccaggg ccatgtgggt gagcgtggga aaccactgat ctatgaagcc cgatgggatc
     3421 agagcaaata cgctgggtcc gacgagaacg cccgccagca gcagcagcgt cacccgcggc
     3481 agcggcgtca ggcgcccggc cacgtcggcc gccagcccga tcaggaacac ggcgccgacg
     3541 atgagcaggg tgttcacgcg ggctcgctct gccggttcag cgggtgccga tggaacacca
     3601 cggtgaagag catgaccagg atgaacacgc caaggagcgt gtagttctcc acgtaggtgg
     3661 actgctccgc caccaggccg taggccttgg cacccagcga ggcgccgagg ttggccgcgg
     3721 acatgtagat ggcgaactgg gtggccgagt tctcccggga gcagatggcc atggcgagcg
     3781 cgatcacgca gaccatgatg accgggtcca tggtcaccca gatggagagc atcacgcgca
     3841 cgtaggtggt gtcctgccac aggtgctggg tctgcgccac gaggaaggcg tgcacggcca
     3901 ccagcgagat ggtcagcacc agcatgcgct tggcgccgaa gcggtcgatg aggggcccgg
     3961 ccgccagcgc caaacccgca cccaccaggc ccatgacggc cacgagctgg gaccactggg
//
LOCUS       DBNHQR010000451         4848 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_485810, whole genome shotgun sequence.
ACCESSION   DBNHQR010000451 DBNHQR010000000
VERSION     DBNHQR010000451.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4848)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4848)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4848
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_485810"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..738)
                     /gene="radC"
                     /locus_tag="AC16VR_10170"
     CDS             complement(64..738)
                     /gene="radC"
                     /locus_tag="AC16VR_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013793377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HIR6011566.1"
                     /translation="MATVKWPDSDQPRNRLLQAGPDRLTDAELLAVCLRTGHGPQDPV
                     TYARSLLRRFGHIGKLLDASPDRLLGMPGMGPAKAAALKASLGVAERYLRAGLGGGRL
                     MGDSRAVTDFLKHQLGSLQREVFACLFLDARHRFIAFEKMFLGSLDRASVHPREVLKR
                     TLELNAAAVIFAHNHPSGVAEPSASDVQITEELKALLERLEVCVLDHVVIGRGCQVSF
                     AERGLL"
     gene            819..2096
                     /gene="coaBC"
                     /locus_tag="AC16VR_10175"
     CDS             819..2096
                     /gene="coaBC"
                     /locus_tag="AC16VR_10175"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017526818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HIR6011567.1"
                     /translation="MTQPDNQAPANLGGRKILLGVSGGIAAYKAPILVRLLRQAGADV
                     QVVLTRSATRFVTATTLQAVSGRPIRDDLWDAAAEAAMGHIELARWADAVLIAPATAE
                     MLSRLATGRADDLLTTLVLATRAPIFVAPAMNNAMWDHPATQRNASTLTDRGVNVLGP
                     DSGEQACGEFGPGRMREPADLVADLCASFEPKDHLLAGKNVLVTAGPTREAIDPVRYV
                     TNHSSGKQGYAMAAAARAAGAQVTLISGPVHLPTPIGVRRIDVTSAAEMHQAVLAHAS
                     TCDVFIGVAAVADYRPDRAHDQKIKKTDTGSGESGLSLRLVENPDIIAGVSSLERRPF
                     VVGFAAETHDALSHARDKRKRKGMDLIVVNDVSDSSIGFNSDQNAATVIWEDGELSIP
                     RQGKDLVARAVIDLIARQIRDPLAGANPERIAK"
     gene            2168..4648
                     /locus_tag="AC16VR_10180"
     CDS             2168..4648
                     /locus_tag="AC16VR_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012020142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HIR6011568.1"
                     /translation="MESDKDDQPAAKKTTGKPAKKRSKPAPTGAFGSTVWAPLLGTLG
                     VVCAFALLWLQLIESGNANHRELQTAVHRDFLVAHFNDRIANLRNQVLSIARTRTVTE
                     AATSEDPATIADAGSALSDLSAFALRVDIFRPGQAVLDKEAEVPISYAALDLIRRAEA
                     AAMVGPEGASINQRGYVYMAAQIGEPGSRAGVLFAVVSPDFFLSGLAWLDERIGRVRI
                     EQLSESVDPIEIFGWGGQSDPAAAEIVTLENQKWNLVFEPGSAILQPVTKMVDLALPF
                     GVALLLVIAGGYLSTTRVTKRLRQAAAAMPVQAQPPEEAPAAETAVEEEAEPVGEEAE
                     PGAGAASTLPSRGFTDDAKDNFGIEVSEEVSLGEMGIDLDPQIFRAYDIRGIVTDNLT
                     EDVVYWIGRAFAAEALAQEQTRAAVGRDGRHSSEALRTALIKGLTDGGLDVADVGEVP
                     TPLLYFATHALDLGTGIMITGSHNPPEYNGLKMMIAGDTLAEERIQRLKERLENNELS
                     EGEGDVEEVDIVRYYIDSALDDIVVAQQLKVVVDCGNGVAGAVAPVLLEEAGCEVVPL
                     YCDVNGDFPNHHPDPADPKNLEDLITVVQAESADLGLAFDGDGDRLGVVTSSGQIIWP
                     DRLLMLFAQDIVSRNPGADIVYDVKCSRHLNAIIGDHGGRPIMWKTGHSHIKAKLKET
                     GALLGGEFSGHICFNDRWYGFDDALYSAARLLEILGAEGGTVDEVFEQFPTGQSTPEL
                     KIQTTEDAKFGIIEELSRSADFGDGTVTSIDGVRVDYPDGWGLIRASNTSPVLTLRFE
                     ADDAEALARIQSDFAVQIAKVDPSLKIS"
     gene            4653..>4848
                     /locus_tag="AC16VR_10185"
     CDS             4653..>4848
                     /locus_tag="AC16VR_10185"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011064225.1"
                     /note="catalyzes the phosphorylation of
                     N-acetyl-L-glutamate to form N-acetyl-L-glutamate
                     5-phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HIR6011569.1"
                     /translation="MPISESQARNIASVLTEALPYIQRFHGGTVVVKYGGNAMVDETL
                     KTTFARDVVLLKLFGLNPPKD"
BASE COUNT          918 a         1623 c         1553 g          754 t
ORIGIN      
        1 tcctggcgaa aaagaggcgc gtttatacca gagcgccctt cggccggcaa cagccggccg
       61 cgttcagagc aggcctcgct cggcgaagga gacctggcaa ccgcgcccga tcacgacgtg
      121 atcgagcacg cacacctcca gccgctccag cagcgctttg agctcttcgg tgatctggac
      181 gtcgctggcg ctcggctcgg cgacgccgga gggatggttg tgagcgaaaa tcaccgccgc
      241 cgcgttgagc tccagagtcc gtttaagcac ctctcttgga tgcacgctgg cccgatccag
      301 cgatccgaga aacatcttct cgaaggcgat gaaccgatgc ctggcgtcca ggaacaggca
      361 ggcgaacact tcccgctgca aactgccgag ctgatgcttg agaaagtcgg tcacggcccg
      421 agagtcaccc atcagacggc cgccgccgag gccggcacgc aggtaccgct cggcgacccc
      481 caggctggct ttcagcgcgg cggctttcgc cggccccatc ccaggcattc cgagcaggcg
      541 atcgggagag gcgtccagca gcttgccgat gtgcccaaaa cgccgaagca gcgagcgcgc
      601 atacgtaacc gggtcctggg ggccgtgccc ggttcttagg cacacggcga gcagctcggc
      661 gtcggtcaga cgatcgggtc cggcctggag aagacgattt cggggctggt cactgtcagg
      721 ccatttgacc gtggccatgg gaaaacctcc tgcggcgaat cggtggcggt ggtatcttta
      781 ggctcaatca gtttccagcc aggcccaaca gctaaacgat gacgcagcca gacaaccagg
      841 cgccggcaaa tctcgggggc agaaagatcc tactcggggt cagcggaggc atcgcggcgt
      901 acaaggcccc aattcttgtg cgccttctgc gacaggccgg ggcagacgtc caggtggttc
      961 tgacccgcag cgcgacgcgg ttcgtcaccg cgactaccct gcaggccgtc tccggcaggc
     1021 ccatcaggga cgacctctgg gacgccgcgg ccgaagccgc catgggccac atcgagctcg
     1081 cccgctgggc cgacgccgtg ctcatcgccc cggcaactgc cgagatgctc agccggcttg
     1141 ccaccggccg cgccgacgac ctgctgacga ccctggttct ggccactaga gcgccgatct
     1201 tcgtcgctcc cgcgatgaac aacgccatgt gggatcaccc ggccacccag cgcaatgcga
     1261 gcaccctgac agaccggggc gtgaacgtgc tggggcccga cagcggcgag caggcgtgcg
     1321 gggaattcgg tccgggccgc atgcgcgagc ccgcggatct ggttgccgac ctgtgtgcgt
     1381 ctttcgagcc taaggaccat ctgctggccg gcaagaacgt cctcgttaca gccgggccca
     1441 cccgcgaggc catcgacccg gttcgctacg taaccaacca cagctccgga aagcaggggt
     1501 acgccatggc cgccgccgca cgggccgccg gtgcccaggt gaccctgatc tccggacccg
     1561 tgcacctccc cacacccatc ggcgtgcggc gcatcgacgt cacgagcgcc gcagagatgc
     1621 accaggccgt cctggcacac gccagcactt gcgacgtgtt catcggcgtg gccgcggtag
     1681 ccgattatcg tcccgaccgc gctcatgacc agaagataaa gaaaaccgac acaggatccg
     1741 gcgaatcggg cctctccttg agactggtcg agaacccgga catcatcgcc ggcgtatcca
     1801 gcctggagcg acgtcctttc gtcgtcgggt tcgccgccga gacccacgat gcgttgagcc
     1861 acgcgcgcga caagcgcaag cgcaagggca tggacctcat cgtcgtcaac gacgtgtcgg
     1921 attcgagcat tggtttcaac agcgaccaga atgctgccac ggtcatctgg gaagacggag
     1981 agctgtcgat tccgcggcag ggcaaggacc tcgttgcccg cgcggtgatc gatctgattg
     2041 cccggcagat acgcgacccg ttagcgggcg caaaccccga acgcatcgca aaatagcgcg
     2101 cggcgctgga ttgccctggc atatccgcat taccattgcc ccgccccgga cgcctggatc
     2161 ctgacccttg gaaagcgaca aagacgacca gcccgccgcc aagaagacca ccggaaagcc
     2221 cgctaagaag agatcaaagc ccgctcccac cggcgcgttc ggctcgacgg tctgggcacc
     2281 gcttctaggc actctcggcg tggtctgcgc cttcgcgctg ctttggctgc agctgatcga
     2341 atccggcaac gccaatcacc gagagttgca gacggccgtg caccgcgact tcctcgtcgc
     2401 ccacttcaac gaccgcatcg ccaatctgcg caatcaggtg ctctccatcg cgagaacgcg
     2461 caccgtcacc gaggcagcca ccagcgaaga cccggccacc atcgccgatg caggcagcgc
     2521 gctgagcgac ctgtccgcct ttgccctgcg cgtggacatc ttccggccag gccaggccgt
     2581 cctggacaaa gaagccgagg tgccaatttc ctacgccgcc ctcgatctga tcagaagagc
     2641 cgaggccgcg gcgatggtgg ggcccgaggg cgcatcgatc aatcagcgcg gttatgtcta
     2701 catggctgcg cagattggag agccgggctc ccgcgccggg gtgttgttcg ccgtggtctc
     2761 tccggacttt ttcctgagcg ggctggcctg gctggatgag cgcatcgggc gcgtgcgcat
     2821 cgagcagctc tccgagagcg tcgaccccat cgagattttc ggctggggcg gccaatccga
     2881 ccccgccgcc gcggagatcg tcacgctgga gaaccagaaa tggaatctcg tctttgagcc
     2941 cggttcggcg attctgcagc cggtgaccaa gatggtcgac ctggcactgc cattcggggt
     3001 cgcccttctg ctggtaatcg ccggcggata cctctccacc acgcgcgtta ccaagcgtct
     3061 gcgccaggcc gccgccgcca tgccggtgca ggcccaaccg cccgaggaag cgccagcggc
     3121 ggagacggcc gtcgaggagg aagccgagcc cgtcggcgag gaggccgagc cgggagcggg
     3181 agcggcaagc acgctgccca gccgcggctt taccgacgac gccaaggaca acttcggcat
     3241 cgaggtcagc gaggaagtgt ctctgggcga gatggggatc gatctggacc cgcagatatt
     3301 ccgggcctac gacattcgcg gcatcgtcac cgataatctg accgaggacg tcgtgtactg
     3361 gatcgggcgg gcgtttgccg ccgaggcgct ggcccaggag cagacccggg cggccgtagg
     3421 ccgcgatggc cgacactcga gcgaggcgct gcgcaccgcg ctcatcaagg gcctgaccga
     3481 tggcggtctc gacgtcgccg atgtcggcga ggtgccgacc ccgctgctct actttgccac
     3541 ccacgctctg gacctgggca cgggcatcat gatcacgggc agccacaacc cacccgagta
     3601 caacgggctg aagatgatga tcgctgggga caccctggcg gaagaacgca tccagcggct
     3661 caaggagcgg ctggagaaca acgagctgtc tgagggcgag ggcgacgtgg aagaagtcga
     3721 catcgtccgc tactacatag acagcgcgct ggacgacatc gtggtcgccc agcagctcaa
     3781 ggtcgtcgtc gactgcggca acggcgtggc gggtgccgtc gcgcccgtgc ttctggagga
     3841 agccggctgc gaggtcgtcc cgctttactg cgacgtcaac ggcgatttcc ccaaccacca
     3901 tccggatccg gcggacccaa agaacctgga agacctgatc accgtggtcc aggccgaatc
     3961 cgccgacctg ggactcgcct tcgacggcga cggcgatcgc ctgggagttg tgacctcttc
     4021 cggccagatc atctggccgg acaggctgct gatgctgttc gcccaggata tcgtgagccg
     4081 gaatccgggt gccgacatcg tctacgacgt gaagtgcagc cggcatctga acgccatcat
     4141 cggagaccac ggcgggcggc ccatcatgtg gaagaccgga cactcccaca tcaaggcgaa
     4201 gctcaaggag accggagcgc tgctgggcgg agagttcagc ggtcacatct gcttcaacga
     4261 tcggtggtac gggttcgacg acgccctcta ctccgcggca cggctgctgg aaatcctcgg
     4321 cgccgagggc ggcaccgtgg acgaggtgtt cgagcagttc cccacggggc aaagcacgcc
     4381 cgagctcaag atccagacca cggaggatgc gaagttcggg atcatcgagg agctcagcag
     4441 gtcggccgat ttcggcgacg gcacggtcac cagcattgac ggggtgcgcg tcgactaccc
     4501 cgacggctgg gggctgatcc gagcctcgaa cacgagcccc gtgctcacgc tacgtttcga
     4561 ggccgacgac gccgaggcgc tcgcgcgcat tcagtcggat ttcgcggtgc agatcgcaaa
     4621 ggtcgacccc tcgctgaaga tctcctgaag ccatgccgat ctccgaatcc caagcccgca
     4681 acatcgcgag cgtactgacc gaggcgcttc cttatataca gcgattccac ggcggcaccg
     4741 tggtggtcaa gtacggcggc aacgccatgg tggacgagac gctcaaaacg accttcgcgc
     4801 gcgacgtcgt gcttctgaag ctgttcggcc tgaatccccc taaggata
//
LOCUS       DBNHQR010000452         5648 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_676585, whole genome shotgun sequence.
ACCESSION   DBNHQR010000452 DBNHQR010000000
VERSION     DBNHQR010000452.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5648)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5648)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5648
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_676585"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="AC16VR_10190"
     CDS             <1..224
                     /locus_tag="AC16VR_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011570.1"
                     /translation="FTDAGLANSQTNAVTVATSDTPVRAAPEDIVPERKSTPTVAQSG
                     SGKSEGQKSGLRGYRSSWARDPDRFKNLN"
     gene            complement(276..1535)
                     /locus_tag="AC16VR_10195"
     CDS             complement(276..1535)
                     /locus_tag="AC16VR_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011571.1"
                     /translation="MWRRHRSWLIGPFLGLWATGSAAQSDFSARVKWFGTATALPEHD
                     LQRNLSGTPAYDQNYDLRLMFQKTISAVTLQVEHSTTLVTGDSLNFPTAPGTVLEQSP
                     EGDERRLMDLTWEIEDGDRHRSFHRLDRLALKLRRDDWAVTVGREAVSWGAGLVFRPM
                     DLFNPFAPTAVDRDYKAGDDLLLIERLFSDGSDLQVLAVGRRDETGDFTGQAGSLAGK
                     WRGFIGACEVELMAAKHYRDQVYGIGVRYPLKGAMLRTDLVATRLADGGARVGGVVNL
                     DYSLEVAGRTTYVFAEYFHNGFGVSELPETVEELPAPLLDRLGRGELFNVMRDYLAGG
                     VTYQWHPLLVQTVTLIGNLNDGSLLLQTQINFDPGDNQRLELGVVEPLGGSGDEFGGV
                     PVVADPAGSRLTVGGGSRLYARWVYYF"
     gene            complement(1519..2220)
                     /locus_tag="AC16VR_10200"
     CDS             complement(1519..2220)
                     /locus_tag="AC16VR_10200"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6011572.1"
                     /translation="MAERVVRCIGVSRTYQDDSVPVHALRDVDFEVGKGEFVTLAGPS
                     GSGKSTLLNMIGGLDRPSSGCVEVDGVDLGSLSESRLSDLRLRKIGFVFQSYNLIPVL
                     SARENVEFIMQLQGVSAAERAARARAALEALGIGELEKRRPGELSGGQQQRVAVARAI
                     VTEPVLLLADEPSANLDSATTRELLEYLRRLNQDRGVTIVAATHDPMVMGYAQRQVGL
                     RDGAIVKDSADVAPA"
     gene            complement(2228..3436)
                     /locus_tag="AC16VR_10205"
     CDS             complement(2228..3436)
                     /locus_tag="AC16VR_10205"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6011573.1"
                     /translation="MLKLLFQLSARNLLRHKRRNLMLLMAVVVAVGGVTLANALIRGF
                     QHDMREAAVQNLTGHLKVLAPGYLDDPGIQRSFTLVQGWSPDIGPRLLQGWAARIRIP
                     AVIMSERETRGVQFVGVDPAQETISFLGDASYRGEPLSGAADRRVVIGQALAEQLDTD
                     VGRRLVLITQGADGLNREAGFRIAGVYDADGQGLEKMFVFTGVTRLQELVDANVFTEV
                     SIKLREEPRGDGLKQGLAETFTDLDVMDWKELEPQTAAMFLFADTAIFIWFCIMMGAL
                     IFGLVNALITAVMERIREFGMLRAVGMRPGSVVVQVVLESTLIMAAGVALGLGLGFLL
                     VVWLSDGIDLTEWAAGVELFGMRSVLVPRLLLADVVLVAAMSIVFGLIASLYPAWRAV
                     QIGPLDALRR"
     gene            complement(3525..4793)
                     /locus_tag="AC16VR_10210"
     CDS             complement(3525..4793)
                     /locus_tag="AC16VR_10210"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6011574.1"
                     /translation="MTQARTPAAGGRARPALLLRIGWRNLARNRRRTWLTAGGIAFAV
                     LLVVFSMAMQLGQYDIMMENATSLLTGHIQIQTADYVDSARFEDTMGSAGALRRVIEA
                     TAGVEAVAPRAEAFALASAGERSFGARIIGVDPDAERDTVRLLKMVREGRAIAAPDDG
                     LLGSLLARNLGVTLGDEVVLLGSEKHGGVAAMVITVAGILETGVADLDRVTLITPLAS
                     VQSAFGLADEVHTFAIRTEDLDRSEAVAESLRHSLTDRPALVVRHWSEVMPELRQAIE
                     IDRLGGELFFYVVEVLVVFSIVNTFIMTVFERTREFGMLKAIGMKPWRIVAMMQWEGF
                     FIWLLGASLGLGLAAIIVAWLQDVGIYMGETLEEYAEQFYMPARLYPAFSLQAFLSAP
                     LVMLMGTQIAALLPSLRIFRLNAAAALRGE"
     gene            complement(4790..5563)
                     /locus_tag="AC16VR_10215"
     CDS             complement(4790..5563)
                     /locus_tag="AC16VR_10215"
                     /inference="COORDINATES: protein motif:HMM:NF028441.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HIR6011575.1"
                     /translation="MHSVKRLLLTAAHSMLVIFPAGVLAAEEPTARELVAAAIDLTRG
                     QSSYAEFAMVIHRPDWQRTSKLVAWTRGRKDALIRFTAPARDAGNATLKQGEKMWTYN
                     PKLNRTIRLPFSLMSQSWAGSDFSYNDLSRTDKLLEEYELAITATRRSEGHTLYTIEA
                     VPREEAPVVWGKEEIVLRDDHVLISQTFFDQAMRPLKRMETLEIGEMGGRIFGTRMRM
                     SKLDEPENYTELSYARVDFDIELEDRIFTLFSLQSGQRP"
BASE COUNT         1061 a         1945 c         1725 g          917 t
ORIGIN      
        1 acttcacgga tgccggcctt gccaacagcc agacgaatgc ggtgaccgtg gctacctccg
       61 acacgcccgt ccgcgccgcc ccggaggaca tcgtccccga gcggaagagc acgccaaccg
      121 ttgcccaatc ggggtccggc aagtcagagg gccagaaaag cggcctgcgc ggctaccgca
      181 gcagctgggc gcgcgatccg gaccgcttca agaatctcaa ctgaggatca tccgtttttg
      241 tgaggcgcca gcgggtcaga ccgtctgccc gcgcctcaga aatagtagac ccaccgcgcg
      301 taaaggcgcg acccgccccc tacggtgaga cgggagcccg ccggatccgc gaccacgggc
      361 accccgccga actcgtctcc tgaaccgccg agcggttcga ccactccgag ctcgagacgc
      421 tggttgtcgc cagggtcgaa gttgatctgc gtctgcagaa gcaggctgcc gtcgttgaga
      481 ttgccaatga gcgtcacggt ctgaaccagc agagggtgcc actggtacgt cactccgccg
      541 gccagatagt cgcgcatgac gttgaacaac tcccctcggc cgagacggtc gagcaggggc
      601 gccgggagtt cttcgacggt ctccggcagc tctgatacgc caaagccgtt gtgaaaatac
      661 tcggcgaaca cgtacgtcgt tcggcccgcg acctccaggc tgtagtccag attgaccacc
      721 ccgccgactc tggcgccgcc gtccgcgagg cgggtggcca ccaggtcggt gcgcagcatg
      781 gctcctttca ggggatatct gacgccaatc ccgtatacct gatcgcggta atgcttggcg
      841 gccatcagct ccacctcgca ggccccgata aacccgcgcc atttgcccgc gaggctcccc
      901 gcctggcccg tgaaatcacc cgtctcgtca cgccgtccca ccgcgagcac ctgcagatcg
      961 ctgccatcgg agaagaggcg ctcgatcagc aggagatcgt cgcccgcctt gtaatcccgg
     1021 tccaccgcgg tgggcgcgaa cgggttgaac aggtccatgg gccggaatac caggccggcg
     1081 ccccagctga cggcctcgcg gccaacggtg acggcccagt catccctgcg cagcttgagg
     1141 gccagccggt cgaggcgatg gaacgagcgg tgccggtcgc cgtcttcgat ctcccaggtc
     1201 aggtccatca gccggcgctc gtctccctcg ggggactgtt cgagaaccgt gccgggtgcc
     1261 gtggggaagt tcagcgaatc tcccgtgacc agcgtggtcg agtgctccac ctgcagcgtt
     1321 accgccgaga tcgtcttctg aaacatcagg cgcaggtcgt aattctggtc gtaggcgggg
     1381 gtgccgctga ggttgcgctg aagatcgtgc tccggcaggg cggtagccgt gccgaaccat
     1441 ttgacccgag cggaaaagtc cgactgcgcc gcgctgcccg tcgcccacag accgagaaac
     1501 ggcccgatca gccagcttct atgccggcgc cacatcggcg ctgtccttca cgatcgcgcc
     1561 gtcgcgcagc ccgacctgac gctgagcgta gcccatgacc atgggatcgt gggtggcggc
     1621 aacgatggtc acgccgcggt cctggttgag gcgacgcaga tactcgagca gctccctggt
     1681 ggtggccgaa tcgagattgg cgctcggctc gtcggccaac agcagaaccg gctcagtgac
     1741 gatggccctc gcaaccgcca cccgctgctg ctgacctccg gacagctccc ccggacgtcg
     1801 cttctccagc tcgccgatgc ccagggcctc gagcgcggcg cgagcccgag cggcacgctc
     1861 cgcggccgac acgccctgca gctgcatgat gaactccacg ttctcccttg cggacagaac
     1921 gggaatgagg ttataggact ggaaaacgaa gccgatcttg cgcagccgca gatccgacag
     1981 ccgactctcg ctgagcgaac cgaggtcaac gccatccacc tcgacgcatc cggagctggg
     2041 gcggtccagc ccgccgatca tgttgagcaa cgtggacttt cccgatccgg acggtcccgc
     2101 cagcgtcacg aactcgccct tgccgacctc gaagtccaca tcccgaagag catgcacggg
     2161 caccgaatcg tcctggtagg tccgggagac cccaatacag cgcactaccc gttcggccat
     2221 ttcttcatca tctccgcaaa gcatccaggg ggccgatctg cacggcacgc catgccgggt
     2281 agagactcgc aatcaggcca aagacaatgc tcatggccgc gaccagcacg acgtccgcga
     2341 ggagaagtct cggaacgagc acgctgcgca taccgaagag ctccacgccg gccgcccatt
     2401 ccgtcaggtc gattccgtcg ctcagccaca cgaccagcag aaaacccaag ccgagcccca
     2461 aggccacccc agcggccatg atgagggtgg attccagcac gacctgcacc accaccgagc
     2521 cgggacgcat gccgacggct cgcagcatgc cgaactctct gatccgctcc atcacggcgg
     2581 tgatcagggc gttgacgagc ccgaatatca aagcgcccat catgatgcag aaccagatga
     2641 agatcgccgt atccgcgaac aggaacatgg ccgccgtctg cggttcgagt tccttccagt
     2701 ccatcacgtc caggtcggta aaggtttccg ccagcccctg cttgaggcca tcgcctcgag
     2761 gctcttcgcg cagcttgatc gacacctcgg tgaagacgtt ggcgtccacg agctcctgaa
     2821 ggcgcgtcac gccggtgaag acgaacatct tctccagccc ctgaccatcg gcgtcgtaaa
     2881 cgcccgcaat ccggaatccg gcttcgcgat tgagcccgtc cgcgccctgt gtgatcagca
     2941 ccaggcgcct gcccacgtcg gtatcgagct gctcggccag cgcctgcccg atcacgaccc
     3001 gccgatcggc tgcaccggat agcggctcgc cgcggtagct ggcatccccc agaaaggaga
     3061 tcgtctcctg cgccgggtcc acgccgacga actgcacgcc gcgggtttcc cgttcgctca
     3121 tgatgacggc cgggatcctg atgcgtgccg cccatccctg aagcagccgg gggccgatgt
     3181 cgggcgacca gccctgcacc agagtgaagc ttctctgaat tcccgggtcg tccagatagc
     3241 cgggcgcaag cactttcaga tgaccggtga ggttctgcac agcggcttct ctcatgtcgt
     3301 gctgaaaacc gcgaatcagc gcgttggcca gggtcacgcc gccaaccgca accactaccg
     3361 ccatgagcag catcaggttc cggcgcttgt gccgaagcag gtttcgagca gagagctgaa
     3421 agagaagctt caacatggac gacaccggcg cggcgacccg aacaccctcc ctgtcctccg
     3481 ggcagccctc gcaccgcccg cgttcgaagc agcgtcaccc gacatcattc gcctctcagc
     3541 gctgcggcgg cgttcagccg gaaaatgcgc agagacggaa ggagcgcggc gatctgggtt
     3601 cccatcagca tgaccagcgg cgcggagaga aacgcctgaa gcgaaaacgc cgggtaaagc
     3661 cgggccggca tgtagaactg ctcggcatac tcctcgagcg tctcgcccat gtatatgccc
     3721 acgtcctgaa gccatgccac gatgatggcg gcgaggccca gccccagcga agccccgagc
     3781 agccagatga agaacccctc ccactgcatc atcgcgacga tgcgccaggg cttcatgccg
     3841 atggctttga gcatcccgaa ttccctggtc cgctcgaaga cggtcatgat gaaggtgttc
     3901 acgatgctga acacgacgag cacctcgacg acgtagaaga agagctcacc cccgagccgg
     3961 tctatctcga tggcttgccg cagctccggc attacctcgc tccaatgcct gaccacgagc
     4021 gcgggccggt cggtcaaact gtgcctcaga ctctcggcga cggcctcgct gcgatccaga
     4081 tcctccgtgc ggatggcgaa agtgtgcacc tcgtcggcaa gcccgaatgc gctctggact
     4141 gacgcgagcg gggtgatgag ggtgacccga tccagatcgg ctaccccggt ctccaggatg
     4201 cccgccacgg tgatcaccat ggcggcgaca ccgccgtgct tctcagatcc caagagcacc
     4261 acctcatcgc cgagagtcac gccgaggttg cgcgccagca aagagcccag caggccgtca
     4321 tccggggcgg caatggctct accctcgcga accatcttca gcaggcgcac ggtatcgcgc
     4381 tcggcgtcgg gatctacgcc gatgatccgg gcaccaaatg accgctctcc cgcggaagcc
     4441 agcgcgaacg cttccgcgcg cggcgcgacg gcctcaacgc cggcggtggc ttcgatcacc
     4501 cgccggagcg caccggcgct ccccatggtg tcctcgaagc gggcgctgtc gacgtagtcg
     4561 gccgtttgaa tctggatgtg acccgtcagc agggaggtgg cgttctccat catgatgtcg
     4621 tactgaccga gctgcatcgc catggagaaa accaccagca gcaccgcaaa cgcgatgcca
     4681 cccgccgtca gccacgtccg cctgcggttg cgcgccaggt tgcgccaacc gattcggagc
     4741 agcagcgccg ggcgggcacg ccccccggcc gccggcgtcc gcgcctgggt cacggccgct
     4801 ggcccgactg cagcgagaag agcgtgaaga ttcggtcttc cagctcgatg tcgaagtcga
     4861 cccgggcgta gctgagctcg gtgtagtttt ccggctcatc gagcttggac atgcgcattc
     4921 gggtaccgaa gatccgcccg cccatctcgc cgatctccag ggtttccatc cgcttgagcg
     4981 gacgcatggc ctgatcgaag aacgtctgcg agatcagaac gtggtcgtcg cgcagcacga
     5041 tctcctcttt gccccacacc accggcgcct cttcgcgggg aaccgcctcg atggtgtaaa
     5101 gggtgtggcc ctcggatcgc cgggtcgccg tgatggccag ctcgtattct tccaacagct
     5161 tgtccgtgcg ggagagatcg ttgtatgaga agtccgagcc tgcccagctc tgcgacatca
     5221 ggctgaaggg caaccggatc gtgcggttca gcttcgggtt gtaggtccac atcttctcgc
     5281 cctgcttgag ggtcgcgttg cccgcatccc tggccggcgc cgtgaaccgg atcagggcgt
     5341 ccttgcggcc gcgggtccag gcgaccagct tcgaggtccg ttgccaatcc ggccggtgaa
     5401 tgaccatggc gaactccgcg taagacgatt gcccccgggt gagatcgatg gcggcggcga
     5461 ccagctcccg agcagtaggc tcttcggcgg cgagaacgcc ggccgggaag atgacgagca
     5521 tcgagtgcgc agcggtgagc agcagccgct tgacgctatg cattgacaag cctccacgag
     5581 caggcaatat ccggatcggc ctcaacgttt gactctatca acaattattg tccccatgcc
     5641 gctgcgag
//
LOCUS       DBNHQR010000453         7752 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_550000, whole genome shotgun sequence.
ACCESSION   DBNHQR010000453 DBNHQR010000000
VERSION     DBNHQR010000453.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7752)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7752)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7752
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_550000"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="AC16VR_10220"
     CDS             <1..492
                     /locus_tag="AC16VR_10220"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR6011576.1"
                     /translation="NVFQAAVEAGVPRVVFASSGATVAGWEHDEPYRALVEGRYDDLP
                     DRWPMITVDAPPRPRGVYGSTKVWGEALARHFADTTATSFISVRIGFVNAEDRPANAR
                     QRSVWCSQRDVVNALVAALGAPEAVRCGTFFANSRNRYGYRDLDHGRDLLGFEPLDAA
                     EAG"
     gene            complement(489..1367)
                     /locus_tag="AC16VR_10225"
     CDS             complement(489..1367)
                     /locus_tag="AC16VR_10225"
                     /inference="COORDINATES: protein motif:HMM:NF004843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6011577.1"
                     /translation="MNDFEGKTAVVTGAASGIGLALAERFAAAQMQVVLADIEEAALD
                     QAVKRLEERQARVIGVVTNTMVESSVAELAERALGEFGNVHVLCNNAGVASLGAGGRG
                     IWEVPAADWDWVMGVNFYGVLFGIQAFVPHMLEHGEPGHIVNTASLAGLFPGGGTYGV
                     SKHAVLALTETLYNDLKARGAALSASVLCPGFVSTRIAKAERNRPAGLEIDQGDVSSE
                     MAAAAEALIAQGKDPAEIAEAVFEAIAQDNLYVLPHPAWDDFVRARVDHVLDRRGPAT
                     MDPAELMRRRAGGEAL"
     gene            complement(1407..3218)
                     /locus_tag="AC16VR_10230"
     CDS             complement(1407..3218)
                     /locus_tag="AC16VR_10230"
                     /inference="COORDINATES: protein motif:HMM:NF012946.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6011578.1"
                     /translation="MSRSEVEGAEFGFDPEALRAKYAEERDKRLRADGNAQYLEVAGK
                     FAHFLDDPYVERTPRDALTDEVEVAIIGGGFGGLLAGARLREAGVEDLRIIEKGGDFG
                     GTWYWNRYPGAQCDIESYIYLPLLEEVGYIPREKYSFAPEIMAHSQAIGRKYDLYRNA
                     CFQTEVTELRWDEEDQRWEIRTNRGDRMKARFVCMSNGPLNRPKLPGIEGIESFQGHT
                     FHTSRWDYAYTGGDPNGNLENLAGKRIGIIGTGATAVQCVPHLAETSEHLHVFQRTPS
                     SIDARGNRPTDPGWAAKLAPGWQRDRMHNFNVLVTGGYQEEDLVSDGWTDIIRNLGII
                     SARRAQTESLTLEDMAKNVELADFQKMEQIRARVDELVDDPETAEALKPYYRQFCKRP
                     CFHDEYLQAFNRPNVTLVDTDGRGVERITDKGIVANGVEYELDCIIFATGFEVGTSYS
                     RRAGYEIVGRNGLTLTDKWADGMKTLHGFQTNGFPNCFFMGVTQGGFTANFPHLLNEQ
                     SIHIAYMIDQARNKNAHVLEASPEAEAGWVETIRQMAVFNQNFLESCTPGYYNNEGNP
                     EVARSLLGSQYGGGPEAFFQILRDWREEGGMEGLDFS"
     gene            3526..4194
                     /locus_tag="AC16VR_10235"
     CDS             3526..4194
                     /locus_tag="AC16VR_10235"
                     /inference="COORDINATES: protein motif:HMM:NF013760.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR6011579.1"
                     /translation="MFKLTDANLVFRALVIFGLVLFGFFLAEQQGLVSLALGSDKSRI
                     SYLILGLYGAVSVHWLWLARALGAERRRFVALENSICAGEAVDVRGGLLGGFLENLSL
                     KRDDDASALLEAFGDELANRHAFGHFASDVLLKLGLLGTVVGFILMLLPVGEIEEFDP
                     SLMQQLLSAMSGGMAVALYTTLAGLVTSTLLKLQYHLLDASAAQLVTRLAVLVDVRLK
                     RDAA"
     gene            4184..4801
                     /locus_tag="AC16VR_10240"
     CDS             4184..4801
                     /locus_tag="AC16VR_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011580.1"
                     /translation="MRHDVFRQRESQDAFTDLLFNALLGFAFMFVAAFALISDPSNTG
                     KIDSKAEVLITVRWADRHPDDVDTLVEGPRGQLVWYHNRDSGLMHLDRDDRGLFADRI
                     VLDGREVSNPINQETVTVRALQPGEYVVNVLHYQSNYSEPLPVTVKVEKLNPTVTLVY
                     YGTRQLTGVGDEQTAVRFTIGADGQVASTSELPKPLLTRAVGGKT"
     gene            4798..5364
                     /locus_tag="AC16VR_10245"
     CDS             4798..5364
                     /locus_tag="AC16VR_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011581.1"
                     /translation="MTGPILGLTLAYVAVAALLLCLNLATRFSPWVKGIAVVLVTALY
                     ASAWLGYQGLLGWASPAALPETFRVLWITVEEPDKESGAPGTIFFWVRELDEAGFPVA
                     PPRAHRIAWDEASARAAQEALEKLEEGERLNGRLSRGVVDEGEGRPEETTGYAAEQSV
                     TGAVGRRPDFEFVQVPPPALPPKAVPGG"
     gene            complement(5368..7590)
                     /locus_tag="AC16VR_10250"
     CDS             complement(5368..7590)
                     /locus_tag="AC16VR_10250"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003239772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HIR6011582.1"
                     /translation="MRAFIWIALVAIAAVVYFFATSTAPDGATEDGPTAAGGGTLESG
                     PSVGLIDDARINAADSEPGNWLAYGRTYEEQRFSPLTQINRESVGELGLAWYKDMGTN
                     RALEATPIVVDGIMFFTTTWSRVHAVDARTGETVWFFDPEVPGETGRKACCDVVNRGV
                     AVYQGKVFVGSLDGRLIALDAATGEKVWEVDTIVDRTRAYTITGAPRAARGKIFIGNG
                     GAEYGVRGYVTAYDAQTGEQVWRFYTVPGDPSKPFEHPEMEMAAKTWKGGEWWEIGGG
                     GTVWNSIVYDPDFNHVYLGVGNGSPWTRVIRSPGGGDNLFLSSIVALNADTGEMQWYY
                     QTTPGDNWDYTAVQDIALADMEVDGEMRKVLLQAPKNGFFYVIDRTNGELLRANPYVA
                     VNWATHVDMATGRPVENPAMDYADTAKWILPGPLGGHNWQAMSVDVEAGLVYIPALEN
                     PLIYGMPDEWQQTGVYKRDPGRWNTGIELGAIAQLFVDNIAEQPTPRGLLKAFDPLSG
                     EERWSVEIPHYWNGGVLGTAGGLVFQGNAMGMFKAYDKSTGEALWEFNTYTSMLAPPV
                     TFELDGEQYVSILTGFGGGDLFGGEPLPPLSMHASLKYNNHGRLLVFKLGAKEQLPVP
                     SARDRTIPEQLVADVDVADIARGERLYNDYCAVCHGLVARSSGAIADLRLMSAESHDA
                     FDGVVLEGLMSARGMTAFSDVLTVEDSERIHHYVRARAHEDREVALGNSEAARLTWLN
                     "
BASE COUNT         1203 a         2527 c         2555 g         1467 t
ORIGIN      
        1 aacgtctttc aggccgccgt ggaggccggc gtgccgcggg tcgttttcgc ctccagcggg
       61 gccacggtgg ccggttggga gcacgacgag ccttaccgcg cgctcgtcga ggggcgctat
      121 gacgacctgc ccgatcgctg gccgatgatc acggtggacg cgcccccgcg accccgcggc
      181 gtttacggca gcaccaaggt ctggggcgag gcgcttgccc gccactttgc cgataccacc
      241 gccacgtcct tcatctcggt ccgcatcggt ttcgtcaacg ccgaggaccg gccggcgaac
      301 gcccgccagc gcagcgtctg gtgcagccag cgggacgtgg tcaacgccct cgtcgcggcc
      361 ctgggcgcgc cggaggcggt gcgttgcggg acttttttcg ccaattctcg aaatcgctat
      421 ggataccgcg atctcgacca cggccgcgat ctgctcgggt tcgaaccgct ggacgccgcg
      481 gaagccggct agagggcctc gccgcccgct cgtctgcgca tgagctcggc cgggtccatg
      541 gttgcggggc cgcgtctgtc cagcacgtga tcgacccggg cgcgaacgaa gtcgtcccat
      601 gccggatggg gcaggacgta gagattgtcc tgggcaatgg cctcgaagac ggcttccgcg
      661 atctcggcgg gatcctttcc ctgggcgatc agggcttcgg cagcggccgc catctcgctg
      721 cttacgtcgc cttggtcgat ctcgagcccc gcgggacggt ttcgctcggc cttggcgatg
      781 cgggtgctga cgaatcccgg gcagaggacg gaggcgctga gcgccgcgcc ccgtgccttg
      841 agatcgttgt acagggtttc cgtcagggcg agcaccgcat gcttgctcac cccgtaggtg
      901 cccccgccgg gaaagaggcc ggccagcgag gcggtgttga cgatgtgccc cggctcacca
      961 tgctcgagca tgtgcggcac gaaggcctgg attccgaaga gcaccccgta gaagttcacg
     1021 cccatgaccc agtcccagtc ggcggccggc acctcccaga tgccccggcc cccggcgccg
     1081 aggctcgcca caccggcgtt gttgcagagc acgtgaacat tgccgaactc cccgagcgcg
     1141 cgttcggcca gctcggccac ggacgactcc accatggtgt tggtgaccac accaatcaca
     1201 cgcgcctgcc gctcttcgag tcgtttgacc gcctggtcca gcgcggcctc ctcgatgtcc
     1261 gccagcacca cctgcatctg cgcggccgcg aaccgttccg ccagggcgag gccgatgccg
     1321 ctcgcggcgc cggtcaccac tgccgtcttg ccttcaaagt cgttcatgtc cgctccctct
     1381 atggtcgaca cccggcgcga gaatcatcag gaaaagtcga gaccctccat gcccccctcc
     1441 tcgcgccagt ccctgagaat ctgaaaaaac gcctccggcc cgccgccgta ctggctgccg
     1501 agcagcgatc gggccacctc cgggttgccc tcgttgttgt agtagccggg cgtgcaggat
     1561 tccagaaagt tctggttgaa aaccgccatc tgccgaatgg tctctaccca gcctgcctcc
     1621 gcttcggggg aggcttccag aacgtgggca ttcttgtttc tcgcctgatc gatcatgtaa
     1681 gcgatgtgga tgctctgctc gttcagaaga tggggaaagt tggcggtaaa accgccttgc
     1741 gtgacgccca tgaaaaagca gttcggaaaa ccgttggtct gaaaaccgtg taaggtcttc
     1801 atgccgtcgg cccacttgtc agtcagcgtc aggccgttcc ggccgacgat ctcgtagccc
     1861 gccctgcggc tgtacgaggt gcctacctcg aagccggtcg cgaagatgat gcagtcgagc
     1921 tcgtactcca cgccgtttgc cacgatccct ttgtccgtga tgcgctccac gccccttccg
     1981 tccgtgtcca ccagggtgac gtttggacgg ttgaatgcct gcaggtactc gtcgtgaaag
     2041 cacggtctct tgcagaactg tcggtaatag ggcttgagag cctcagccgt ttccggatcg
     2101 tcgacaagct cgtccacgcg cgcccggatc tgctccatct tctggaagtc ggccagctcc
     2161 acgttctttg ccatgtcctc gagcgtcagg ctctcggtct gagcgcggcg ggcggagatg
     2221 atgcccaggt tgcggatgat gtcggtccag ccatcgctga ccagatcttc ctcctgatag
     2281 ccgccggtca cgagcacgtt gaagttgtgc atgcggtccc gctgccagcc cggcgcgagc
     2341 tttgccgccc agcccgggtc ggtaggccgg ttgccgcgcg catcgatgga tgatggcgtt
     2401 cgctggaaca cgtgaaggtg ttcagaggtc tcggccagat gcggcacgca ctgaacggcg
     2461 gtggctccgg tgccgatgat gccgatgcgt ttgcccgcca ggttctcgag gttgccgttc
     2521 gggtcgccgc cggtgtaggc gtagtcccag cggctggtgt gaaacgtgtg gccctggaac
     2581 gattcgatgc cttcgatgcc gggcagcttc ggccggttga gcggaccgtt ggacatgcag
     2641 acgaaacgag ccttcatccg atcaccgcgg ttagtgcgga tctcccaccg ctggtcctcc
     2701 tcgtcccagc gaagctccgt gacttcggtc tggaagcagg catttcggta aaggtcgtac
     2761 ttgcggccga tggcctggct gtgggccata atctccggcg cgaagctgta cttctccctg
     2821 gggatgtagc cgacttcctc gagcagcggg agatagatgt acgactcgat gtcgcactgg
     2881 gcgcccgggt aacggttcca gtaccacgtg cccccgaaat ccccgccctt ctcgatgatc
     2941 ctcagatcct cgacgcctgc ttcccgaagc cgggcgccgg caagcaggcc gccaaacccg
     3001 ccgccgatga tggcgacttc gacctcgtcg gtcagggcgt cccttggtgt gcgctccacg
     3061 tagggatcgt ccaggaaatg ggcgaacttg ccggcaactt ccaagtactg ggcgttgccg
     3121 tcggcgcgca ggcgcttgtc ccgctcctct gcgtattttg ctcgcagggc ttccgggtcg
     3181 aacccgaact ccgccccttc gacttctgac ctgctcatgg gtcgacctcc aactgcacgc
     3241 ggaaaagcgg cctatttagg aatgccgcta tgtagattgc aaccttcgct ggcgggagcg
     3301 tatgcggccg tcggggttgg acggggatgc ggcttcttga cacctgccgg cgtccttggt
     3361 cgcattgtgc agtccatcgg tcggcgggga ggggcaccgt gattggggga agctgtcagt
     3421 ttggtgccgt gagctttgag cttgcggctg atccagcgag catttgcgag ctgcccgtcc
     3481 ggcatatcga cggggctgaa acccggcagt tcgtggatta gggccatgtt caagctcaca
     3541 gacgccaacc tcgtctttcg cgccctcgtc atcttcggtc tcgtgctgtt cgggtttttt
     3601 ctggccgagc agcagggcct ggtgagcctg gctctcggct ccgataagag ccgcatctca
     3661 tacctcattc tcggtcttta cggcgctgtg agcgttcact ggctgtggct ggcccgcgcg
     3721 ctgggtgcgg aacggcggcg cttcgtcgcg ctggagaaca gcatctgcgc aggtgaggcc
     3781 gtcgacgtca gaggcggtct gctcggcggg tttctcgaga acctctccct caagcgcgat
     3841 gacgacgcct cggctctgct cgaagcgttt ggcgacgagc tggcgaaccg gcacgcgttc
     3901 ggtcatttcg ccagcgacgt tctgttgaag ctcggcctgc tcggcaccgt ggtcggcttc
     3961 atcctcatgc tcctgcccgt cggggagata gaagagttcg acccgagcct catgcagcag
     4021 ctgctgagtg ccatgagcgg cggcatggcg gtagcgctct acacgacgct ggccgggttg
     4081 gtcaccagca ccctgctcaa gctgcaatat cacctgctcg atgcgtcggc cgctcagctc
     4141 gtcacgcggc ttgcggtgct ggtggacgtg aggctgaagc gcgatgcggc atgacgtctt
     4201 ccggcagcgc gaatctcagg acgctttcac cgacctgctt ttcaacgcgc tgctgggctt
     4261 cgccttcatg ttcgtcgccg cgttcgccct gatcagcgat ccgagcaaca cggggaagat
     4321 cgactcgaaa gccgaggtgc tcatcaccgt gaggtgggcc gaccggcacc cggacgatgt
     4381 cgataccctg gtcgaggggc caagagggca gctggtctgg taccacaatc gcgacagcgg
     4441 gctcatgcac ctggaccgcg acgatcgggg cctgttcgcg gatcggatcg ttctggacgg
     4501 ccgggaggtt tccaacccga tcaatcagga gacggtgacc gttcgcgcgc tgcagccggg
     4561 tgagtacgtg gtgaacgtgc tccactatca gtcgaactac agcgagccgc tgcccgtcac
     4621 cgtgaaggtg gagaagctca accccacggt tacgctcgtc tactacggca cccggcagct
     4681 caccggggtg ggggacgagc agacggccgt tcggttcacc atcggcgccg acggacaggt
     4741 cgcgagcacc agcgagctgc cgaagccgct cctgacccgg gcagtgggcg gcaagacgtg
     4801 accggcccga ttctcgggct caccctggcc tacgtggcag tggccgccct gctcctgtgc
     4861 ctgaacctgg cgacccgctt ctcgccgtgg gtcaagggaa ttgcggtggt gttggtcact
     4921 gcgctttacg cgtcggcctg gctcggctac cagggtctgc tcggctgggc atcgcctgct
     4981 gcgctgccgg agacgttccg cgtgttgtgg atcacggtgg aggagccgga caaggaaagc
     5041 ggcgcccccg gcaccatctt tttctgggtg cgtgagctgg acgaggcggg ttttccggtc
     5101 gcccccccca gagcgcaccg gattgcctgg gacgaagcgt ctgccagggc ggcccaggag
     5161 gctcttgaaa agctggagga gggggagcgg ctcaatgggc gtttgagccg aggcgtggtg
     5221 gacgagggcg aaggccgtcc cgaagagacc accggctacg cggccgagca gtccgtgacg
     5281 ggcgccgttg ggcgccgccc ggacttcgag ttcgtgcagg tcccgccgcc ggcgctgccg
     5341 cccaaggcgg tcccgggcgg gtagcggtca gttcagccag gtgagcctgg cagcctccga
     5401 gtttccgagc gccacttccc ggtcctcgtg tgcccgggct ctgacgtagt ggtgaatgcg
     5461 ttcggaatcc tccaccgtga gcacgtcgct gaacgccgtc atgccccttg cggacatcag
     5521 cccctcgaga acgaccccgt cgaaggcgtc gtgggactcc gcggacatca ggcgcaaatc
     5581 ggcgatggcc ccgctggagc gtgcgaccag cccgtggcag acggcgcagt agtcgttgta
     5641 gaggcgctcg ccccgggcga tgtccgccac gtcgacgtcg gcgaccagct gctccggaat
     5701 ggtccggtca cgggccgagg gaaccggcag ctgctctttg gcgccaagct tgaacaccag
     5761 caaacggccg tggttgttgt acttcagcga agcgtgcatc gacagcggcg gcaggggctc
     5821 gccgccgaac aggtcgccgc cgccgaaccc ggtgaggatc gatacgtatt gctcgccgtc
     5881 cagctcgaaa gtgacgggtg gtgcgagcat ggaggtgtaa gtgttgaact cccacagcgc
     5941 ctcgccggtg gatttgtcgt aggccttgaa catccccatg gcgttgccct ggaagaccag
     6001 cccgccggcg gtgccgagca cgccgccgtt ccagtagtgt gggatctcca ccgaccagcg
     6061 ctcctcgcct gagagcgggt cgaaggcttt gagcagaccg cgaggcgtgg gctgctcggc
     6121 gatgttgtcc acgaacagct gcgcgatggc gccgagctcg atccccgtgt tccagcggcc
     6181 cgggtcgcgc ttgtagacgc ccgtctgctg ccactcatcc ggcatgccgt agatcagcgg
     6241 gttctccagc gccgggatgt agaccaaacc ggcctccacg tccacggaca tggcctgcca
     6301 gttgtgcccg ccgagcggtc ccgggagtat ccacttcgct gtgtcggcgt aatccatggc
     6361 cggattttcc actggccggc cggttgccat gtccacgtgg gtggcccagt tcaccgcgac
     6421 gtagggattg gcgcgcagaa gctcgccgtt ggtccggtcg atgacgtaga agaagccgtt
     6481 cttgggcgcc tggagaagca ccttgcgcat ctctccgtcc acttccatgt cggcaagcgc
     6541 gatgtcctga acggccgtgt agtcccagtt gtcgcccggg gtggtctggt agtaccactg
     6601 catctcgccg gtgtccgcgt tcagggcgac gatggaggag aggaacaggt tgtcgccgcc
     6661 gcccggtgat cggatcaccc gcgtccaggg ggaaccgttg cccacgccga ggtagacgtg
     6721 gttgaagtcc gggtcgtaga cgatggagtt ccacaccgtg ccgccaccgc cgatctccca
     6781 ccactcgccg cccttccagg tcttcgcggc catctccatc tccggatgct cgaagggctt
     6841 ggagggatcg ccgggcaccg tgtagaagcg ccagacctgc tcgccggtct gggcatcgta
     6901 ggcggtgacg tagccgcgca cgccgtactc ggcgccgccg ttgcctatga agatcttgcc
     6961 ccgtgccgct cgcggcgcgc cggtgatggt gtaggcccgg gtacgatcga cgatggtgtc
     7021 cacttcccat accttctcgc cggtggccgc gtccagcgcg atgagccggc cgtccaggct
     7081 gccgacgaag accttgccct gataaacggc gaccccgcgg ttgaccacgt cgcagcaggc
     7141 tttgcgtccg gtctctcccg gcacctcggg atcgaagaac cagaccgtct ccccggtccg
     7201 tgcgtccacc gcgtggacgc ggctccaggt ggtggtgaag aacatgatgc cgtccacgac
     7261 gatgggcgtg gcctccagcg cacggttggt gcccatgtcc ttgtaccagg cgaggcccag
     7321 ctccccgacg ctttcccggt tgatctgagt cagcggcgag aagcgctgct cctcgtaggt
     7381 gcgcccgtag gccagccagt ttccgggctc gctgtcggcg gcgttgatgc gcgcgtcgtc
     7441 tatcagcccg acggacggcc cagattccag agtgccgccg ccggccgcgg tcggcccatc
     7501 ctccgttgcc ccgtcaggag cggtggatgt cgcgaaaaag tagaccacag cagcgatcgc
     7561 gaccaatgcg atccagatga acgcgcgcat gatgtctccc tttacctgcg tcggggatca
     7621 taccgccgct ctcccgaccg cgacaaactt cgtgcgcggc gtatgccgcg gggttcgcgc
     7681 tgcgcttctg gcgggcgtgt ggttcgaggg atagactgca attgggaggg ttgccatgaa
     7741 gagctggatc ga
//
LOCUS       DBNHQR010000454         2495 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1626327, whole genome shotgun sequence.
ACCESSION   DBNHQR010000454 DBNHQR010000000
VERSION     DBNHQR010000454.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1626327"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="AC16VR_10255"
     CDS             complement(<1..284)
                     /locus_tag="AC16VR_10255"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KdsC family phosphatase"
                     /protein_id="HIR6011583.1"
                     /translation="MAFPAPVLERAADIELVAFDVDGVLTDGKLYYSDDGRELKAFHV
                     QDGAALKLLLSHGIVVAIITGRSSSIVTRRADELGIAHVYQGVADKAAVLA"
     gene            complement(284..1255)
                     /locus_tag="AC16VR_10260"
     CDS             complement(284..1255)
                     /locus_tag="AC16VR_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HIR6011584.1"
                     /translation="MLLSIAALIVGFMALLWSADRFLAGAASTAGHLGVSKMLIGLTV
                     VSLGTSAPEILVALTAAVEGNPLLAVGNAIGSNIANIGLVLGITAIIVPLPFSQTVLR
                     AELPWLLGATFLALALLFDLEMSALDSVLLLAGLGVILWRLVRSQKTAGSDLAESITE
                     ELEDLPQMSRGQAAVWLLIGLLILLASAEVLVWAASDIADRLGVNDIIIGLTIVAIGT
                     SLPELAATVGSALKGHTDIAIGNVVGSNILNILAVLAVPSLVDPILIENVWLWRDGGM
                     MLALTLLLAVFAYAVGSQPVITRFEGTVMLCAWVGYNLLLVSQAQLG"
     gene            1384..2199
                     /locus_tag="AC16VR_10265"
     CDS             1384..2199
                     /locus_tag="AC16VR_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6011585.1"
                     /translation="MNGADDNLIEIRNLSFVRGDRVIFDDLSLSVPRGKITAIMGPSG
                     TGKTTLLRIIGGQVRPDSGVVLIDGQDVCGMSRRELFELRKDMGMLFQSSALFTDLNV
                     FENVAFPLRVHTDLPEDTVRDLVLMKLNAVGLRGARDLMPSQLSGGMSRRVALARAVA
                     LDPNLLMYDEPFTGQDPIAMGVLLSLIESLNDSLKTTSLVVSHDIHETISIADQIYLI
                     ADGTIMGSGTPDTLAVDDSPRVHQFMHGSPDGPVPFHYPADEFLYDVLGDVNA"
     gene            2196..>2495
                     /locus_tag="AC16VR_10270"
     CDS             2196..>2495
                     /locus_tag="AC16VR_10270"
                     /inference="COORDINATES: protein motif:HMM:NF014460.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6011586.1"
                     /translation="MIQFLRSFGGRSLDRMASLGRATIMWFTALAGLPGPGDLRLLVR
                     QIYHVGVLSLIIIVLSAFSIGAVIALQFHTQLARFGAEDSVGLGLALVLLRELGPV"
BASE COUNT          437 a          819 c          806 g          433 t
ORIGIN      
        1 gccaggacgg cggccttgtc cgcgaccccc tggtagacgt gggcgatgcc cagctcgtcg
       61 gctcgtctcg tgacgatgct ggaagaccgg cccgtgatga tcgccaccac gatgccgtgg
      121 gaaagcagga gcttcaaagc ggcgccgtcc tggacatgga aggccttcag ctcgcgaccg
      181 tcgtcgctgt agtagagctt gccgtcggtg agcacgccgt ccacatcgaa agccaccagc
      241 tcgatatccg ccgcgcgctc gagcaccggc gccgggaaag ccatcagccc agctgtgcct
      301 gagacacgag cagcaggttg tagccgaccc aggcgcacag catgaccgtg ccctcgaagc
      361 gggtgatcac gggctgggac ccgacggcgt aggcgaacac cgcgagcagg agcgtcagcg
      421 ccagcatcat gccgccgtcc cgccacagcc agacgttctc gatgaggatc gggtccacca
      481 ggctggggac ggccagcacc gccaggatgt tcagaatgtt ggatcccacg acgttgccga
      541 tggcgatgtc cgtgtggcct ttcagcgcgg agcccaccgt tgccgcaagc tctggaaggc
      601 tggtgccgat ggcgacgatt gtcaggccga tgatgatgtc gttgacgccc agccggtcgg
      661 cgatgtcgct ggcggcccag accagcacct cggcgctggc cagcaggatc agcaacccga
      721 tcagcagcca cacggctgcc tggccgcgcg acatctgcgg aagatcctcc agctcctcgg
      781 tgatgctttc tgccaggtcg cttcccgcgg tcttttgact gcgcaccagg cgccacagaa
      841 tcacgcccag cccggccagc agcagcaccg agtccaatgc ggacatctcg agatcgaaga
      901 gaagagccag ggccagaaag gtggcgccca gcagccaggg cagctcggcg cgcagcacgg
      961 tctgggaaaa gggcagcggc acgatgatcg cggtgatgcc cagcaccagg ccgatgtttg
     1021 cgatgttcga gccgatggcg ttgcccacgg cgagcagcgg gttgccttcc accgcagccg
     1081 tcagggccac gagtatctcg ggggccgacg tgccgaggga gaccaccgtg aggccgatga
     1141 gcatcttgga cacaccgagg tgcccggcgg tgcttgccgc accggcgaga aagcgatcgg
     1201 cgctccacag cagtgccatg aagccaacga tgagggcggc tatcgagagc agcatgagct
     1261 ggcgccggcc gggttatggg gatcaatagt caagttggcc actttagtct catcgcgccc
     1321 gtcagccaac tccagcccgc gatgttatag ttgcgcccgt cttgctcacg accccagacg
     1381 ttcatgaacg gcgccgacga caacctcatt gagatccgca atctctcttt cgtccgcggt
     1441 gaccgggtca ttttcgatga tcttagcctg tcggtgcccc gcggcaagat cactgcgatc
     1501 atgggcccga gcggcacggg caagacgacc ctgctgcgaa tcatcggagg ccaggtgcgc
     1561 cccgacagcg gggtcgtgct catcgatggt caggacgtct gcgggatgag ccgacgcgag
     1621 ctgttcgagc tgcgtaagga catggggatg ctgttccagt cgtccgcgct gttcaccgac
     1681 ctgaacgtct tcgagaacgt ggcgtttccc ctgcgcgttc acaccgatct ccccgaggac
     1741 accgtacgcg acctcgttct gatgaagctg aacgcggtag ggctgcgcgg cgcgagagat
     1801 ctcatgccga gccagctttc cggtggcatg tcgcggcggg tcgccctggc acgtgccgtc
     1861 gcgctggatc cgaacctcct gatgtacgac gaacccttca ccgggcagga tccgatcgcc
     1921 atgggggtgc tgctcagcct catcgagagc ctcaacgact cgctgaagac gacgagcctc
     1981 gtggtttctc acgacattca cgagaccatc agcattgccg accagatcta tctcatcgcc
     2041 gacggcacga tcatgggatc cggcacgccc gatactctgg cggtggacga ctcgccacgc
     2101 gtccaccagt tcatgcacgg ctcgccggac ggccccgtgc cctttcacta tcctgccgac
     2161 gagttcctct acgacgttct aggggacgtg aacgcgtgat ccagttcctg cgcagcttcg
     2221 gtggccgcag cctggaccgg atggcgagcc tcggcagggc gaccatcatg tggttcacgg
     2281 cgctcgcggg gctcccgggg cccggggacc tgcgcctgct cgttcgccag atctaccacg
     2341 tgggcgtgct gtccctcatc atcatcgttc tttcggcgtt ctccatcggt gcggtgatcg
     2401 ctctgcagtt tcacacccag ctggcgcgtt tcggtgccga ggactcggtg ggtctcgggc
     2461 tcgcgctggt gctgctgcgc gagctcgggc cggtg
//
LOCUS       DBNHQR010000455         2611 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_613350, whole genome shotgun sequence.
ACCESSION   DBNHQR010000455 DBNHQR010000000
VERSION     DBNHQR010000455.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_613350"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="AC16VR_10275"
     CDS             complement(<1..454)
                     /locus_tag="AC16VR_10275"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6011587.1"
                     /translation="MFLEVFRFENRFQVRSPLFLTISTVWFLFGFLITGSESVSVGGV
                     GSNLNLNASFAIIQTITVLTIIGMFAAIAFVASAITRDRETKTAEIFYSSGISERSYL
                     LGRFAGGCVFATAAVTAGVVGTMLGTFMPWLDAERIGSFSLAPYVFSML"
     gene            complement(466..1353)
                     /locus_tag="AC16VR_10280"
     CDS             complement(466..1353)
                     /locus_tag="AC16VR_10280"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017737462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_function: GO:0140657 - ATP-dependent activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6011588.1"
                     /translation="MLKINALSKTYPNGVQALDGVDLEIAKGMFGLLGPNGAGKSTLM
                     RTIATLQSPDSGSIHFNDVDVLADPNALRRVLGYLPQEFGAYPRTSPEVMLDHIAALK
                     GIGGRGARKALVEQLLVQTNLWDVRKKSIDSFSGGMRQRFGIAQALIGDPQLVIVDEP
                     TAGLDPAERRRFHNLLARIGEEVVVILSTHIVEDVAELCPNMAVMGGGRILVQGEPKE
                     LTGRLAGRLWSKVVPRDEAEGLRRSLPVVSTRLVAGQVELRVLSEQDPEGGLAAVEPS
                     LEDVYFATLMKHGMNPVVD"
     gene            1514..>2611
                     /locus_tag="AC16VR_10285"
     CDS             1514..>2611
                     /locus_tag="AC16VR_10285"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HIR6011589.1"
                     /translation="MSELARQAALDTAGMIRRGDASAVEVMDAHYDRIEILNPQINAL
                     VNVLPREAALELARQADRARSRGEPCGPLHGLPMAVKDTHDAAGFPTTWGFVPWAERV
                     ARHDGAVAARQRAAGALFIAKSNVPEFALGSHTFNNLFGTTSNPYDLSRTAGGSSGGA
                     AAALATGMLPLADGSDMGGSLRNPAGYCNVVGLRPSLGRVPADGPFGWFARLLTPGPM
                     ARNVPDAALLLSVQAGPNQDDPLSRPEPGRHFRDLPHADPATLKIAWSADLGFAPVDA
                     AMVRLLEAAAVVFEDLGCEVENACPDLRGGMSVFQTQRAASLAQTGRALDRSLPRWRE
                     HAKETMIWNVEQGYALTAEDLLRCEVERTAIY"
BASE COUNT          474 a          892 c          811 g          434 t
ORIGIN      
        1 ccagcatgct gaacacataa ggcgccaggc tgaagctgcc gatacgctcc gcatcgagcc
       61 agggcatgaa ggtgcccagc atggtcccga cgacgccggc ggtcaccgcg gcggtggcga
      121 agacgcaccc cccggcaaat cgtcccagca gatagctcct ctcggatatc ccgctgctgt
      181 agaagatctc ggccgtcttg gtttcgcgat cgcgcgtgat ggcgctggcc acgaaggcta
      241 ttgccgcaaa catgccgatg atggtgagca ccgtgatcgt ctggatgatg gcaaacgacg
      301 cgttcaggtt caggttgctg ccgacaccgc cgacgctcac cgattcgctg ccggtgatca
      361 gaaaaccaaa aaggaaccac accgtggata tcgtcaggaa cagcggggag cggacctgaa
      421 agcggttttc gaatctgaag acctcgagaa acatggcgcc tcctctcagt cgaccaccgg
      481 gttcatgccg tgcttcatga gggtggcgaa gtaaacgtcc tccaggctcg gctcgaccgc
      541 ggcgaggcca ccctcggggt cctgctccga gagcaccctt agttccacct gccccgccac
      601 cagtcgcgtc gacaccaccg gcagggaacg ccgcaaccct tcggcctcgt cgcggggaac
      661 cactttcgac cacagccgtc ccgcaagacg cccggtcagc tccttgggtt caccctgcac
      721 cagaatccgc ccgccgccca tgacggccat gttcgggcaa agctcggcaa cgtcctcgac
      781 gatgtgggtg gagagaatga ccaccacctc ctcgccgatt ctggccagca ggttatgaaa
      841 gcgccggcgc tctgcgggat cgagccccgc cgtgggctcg tcgacgatca ccagctgcgg
      901 atcgccgatg agcgcctggg cgatcccgaa gcgttggcgc atacccccgg aaaagctgtc
      961 gatgctcttc ttgcgcacgt cccagaggtt cgtctgcacc agcagctgct cgacgagcgc
     1021 tttgcgagcg ccgcgaccgc caatgccctt gagcgctgcg atgtgatcga gcatcacttc
     1081 cggagacgtg cgcggatacg cgccgaactc ctgcgggagg tagcccagca cgcgacgcag
     1141 ggcgttggga tccgcgagca catcgacgtc gttgaagtga atggaaccgc tgtccgggct
     1201 ctgcagcgtc gcgatggtgc gcatgagcgt ggacttgccg gcaccgttcg gcccgagcaa
     1261 cccgaacatg cccttggcga tctccagatc cacgccgtcc agcgcctgca caccgttggg
     1321 ataggtcttc gacaaagcgt ttatcttcag catgctttct ccttgcagag ctcgatcggt
     1381 cgttcctcga cgcccgagaa ccaccgcgct cccggcgcgg cccgttccac caacagttcg
     1441 accatgccgg ccaaccgcaa tgcgtgcgcg ccaccacctt gccggcgcgc gttggcgaat
     1501 cgagaatacc tcaatgagcg aactggcgcg gcaagcggct cttgatactg ccgggatgat
     1561 tcgacgggga gacgcatccg ccgtggaagt catggacgca cactacgatc gcatcgaaat
     1621 cctgaacccc cagatcaatg cgctcgtcaa cgttctgccc agagaggcgg cactcgagct
     1681 ggctcgccaa gcggatcggg cgcgcagccg cggtgagccg tgcggtcctc tgcacggtct
     1741 gcccatggcc gtcaaggaca cccatgacgc cgcaggcttt ccgaccacct ggggatttgt
     1801 cccctgggcc gaacgcgtgg ctaggcacga tggagcggtg gccgcacgcc aacgggccgc
     1861 aggcgcactt ttcatcgcga aatccaacgt gcccgagttc gccctgggat cacacacgtt
     1921 caataacctc ttcggaacca cgagcaatcc ctacgacctg tcgcgcacgg ccggaggcag
     1981 cagtggcggc gccgccgcgg cgctggcaac ggggatgctt cccctggccg acgggtcgga
     2041 catgggcgga tcgctgcgca acccggccgg gtactgcaac gttgtcggcc ttaggccctc
     2101 attggggcgc gtcccggcgg atggcccgtt cggctggttt gctcggctgc tcacccccgg
     2161 ccccatggct cgtaacgtgc ccgacgcggc tctgctgctg tccgttcagg caggccccaa
     2221 tcaggacgac cccctgagcc ggccggagcc gggcagacac ttccgcgatc tgccccacgc
     2281 cgatccggca accctgaaga tcgcctggtc cgcagatctc ggctttgcac ccgtggacgc
     2341 cgccatggtc cggctgctcg aggcagcggc ggtcgttttc gaagacctcg gctgcgaggt
     2401 ggagaatgcc tgtccggacc tgcgcggagg catgtcggta ttccagaccc agcgcgcggc
     2461 cagcctggcc cagaccggcc gggccctgga tcgctcgcta ccgcgctggc gcgaacatgc
     2521 caaggaaacc atgatctgga acgtggagca gggctacgcg ctcaccgcag aggatctgtt
     2581 gcgctgcgag gtcgaacgta cggcgattta c
//
LOCUS       DBNHQR010000456        11753 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1499318, whole genome shotgun sequence.
ACCESSION   DBNHQR010000456 DBNHQR010000000
VERSION     DBNHQR010000456.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 11753)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11753)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11753
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1499318"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="AC16VR_10290"
     CDS             complement(<1..511)
                     /locus_tag="AC16VR_10290"
                     /inference="COORDINATES: protein motif:HMM:NF024758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HIR6011590.1"
                     /translation="MRHTLKCAVFGWLLLGSLTAAAALPLGLPGGEQLPSLAPMLDQA
                     TPAVVNIATYTTVQVRNPLLEDPFFRRFFNVPDQRRYRRTQSAGSGVVIDAGKGFIVT
                     NNHVVERADEISVTLADGRSMTAELVGSDPQVDLAVLKVEADDLQQIGFADSGELRVG
                     DFVVAIGNPF"
     gene            642..1070
                     /locus_tag="AC16VR_10295"
     CDS             642..1070
                     /locus_tag="AC16VR_10295"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR6011591.1"
                     /translation="MPIKNVFEAIQREKANIENLSVEELKQALADDPDLLLLDIREIQ
                     ERVDLGTIPGSVHVPRGMLEFWADPASPYFRDYFQEDRRTVVFCAGGGRSVLAVRALQ
                     DMGFTDVAHLEPGFGGWKKAAEPIEDVASSSRWVRRADDD"
     gene            1146..1221
                     /locus_tag="AC16VR_10300"
     tRNA            1146..1221
                     /locus_tag="AC16VR_10300"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1179..1181,aa:Gly,seq:ccc)
     gene            complement(1225..3006)
                     /gene="pabB"
                     /locus_tag="AC16VR_10305"
     CDS             complement(1225..3006)
                     /gene="pabB"
                     /locus_tag="AC16VR_10305"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - aminodeoxychorismate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008153 - 4-aminobenzoate biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HIR6011592.1"
                     /translation="MSTDVIVYDAVAQQWLRFCDPVELLIADREDQVLDVVAEIESRT
                     VGDGLFAAGFLTYEAAPGIARELVTHRPHRLLPLAWFGLFRRVLPSADLPLAPGDCLG
                     GWSASVTRKDYDSAIARIRDYIAAGDTYQVNYSFRLRARLNDSPAALFANMVSSQGPG
                     LAAFIDTPGFAVASASHELFFARDGDELVSRPMKGTAPRGLRDQTDRDQAGWLAASAK
                     NRAENLMITDMVRNDLGRIARIGSVVADDLFALERYPTVWQMTSTVRARSTASLPDVM
                     GALFPAASITGAPKRRTMEIIRELEHEPRGIYTGTIGFVRPEGRCQFNVAIRTAAIDK
                     TTSEAEYGVGGGIVWDSRAEDEYRECFAKARIVTGGRREFELLETLAWHPRDGFRRLE
                     EHLERLHSAADYFCWSLSTGKAQALLEAAASAYREPMRVRLLADRSGHVRLEASPLKP
                     LPEPYRVALSEPMEDLQSEFVYHKTTQRSVYDKALDAARSVRPDLDDVLLVNGDGQVT
                     ESTIANVAVEVDGRLLTPELGCGLLPGIERQVLVADKILEEARVPVSLLVEAERIWLL
                     NSVRGMWPATLVATEQGGSDLRSEFPG"
     gene            complement(3128..4093)
                     /locus_tag="AC16VR_10310"
     CDS             complement(3128..4093)
                     /locus_tag="AC16VR_10310"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6011593.1"
                     /translation="MRPPALTWLILGSLLAVLSLPAISSEPARPLSAHHVIVDGVPLA
                     VWRKAPASAKRVVLLVHGRTWSARPNFDLNVPGEDLSLMDGLVEEGIATYALDMRGYG
                     DTPRDASGWLTPSRAAADVIAVLEWIAQRNPGLPGPYLFGYSRGAMVAQLAAQQQPGR
                     ISGLIVFGYPLRQDSAVNPPGLTTEPARIANSAADARSDFIAPGAARRETIDAFVESA
                     LKHDPVRVDWRAYPEWLALDAARVTVPTLLVDAEHDPLTDPKVLSRFFAGLATADKAW
                     VSIPGADHAAFLEEPRDYFRRAVAAFMLGERAAVEVISADLLDFD"
     gene            4251..5024
                     /locus_tag="AC16VR_10315"
     CDS             4251..5024
                     /locus_tag="AC16VR_10315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6011594.1"
                     /translation="MDVSGKIVVVTGGASGIGRAMAQRFHAEGAGKVIVADLDGDGAR
                     RVADEIDGSSMQVDVGREADLVALIEHAESEFGPIDLFCSNAGIGIGKGLDEPDEVWD
                     TIWKVNTMSHVYAARHMVPRMKARGGGYLLNTASAAGLLTQIGSVTYAVTKHAAVALA
                     EWIAITHARDGIKVSVLCPQAVRTPMTADGPGVAGVDGMIEPEEAAECVIEALREERF
                     LVLPHPEVATYIQRKTSDYDRWLNGMRRLQDLYVAEEGE"
     gene            complement(5074..5871)
                     /locus_tag="AC16VR_10320"
     CDS             complement(5074..5871)
                     /locus_tag="AC16VR_10320"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013134.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR6011595.1"
                     /translation="MYQFNYHRPASVEEAVGLFESMDDGIYMAGGQTLIPTMKQRLAA
                     PTDVIDLSGIEALSGIEVSADRVSIGAFTVHDAVASSPEIRATLPVLAFLAGQIGDGQ
                     VRNRGTLGGSVANSDPAADYPAALIGLGATVHTQTRSIEADDYFKDLFETALNPGELI
                     TRIDFPVPTRAAYRKFPNPASRYAVVGVLVADFGGKVRVGVTGAGPCAFRATAIEELL
                     SRNLDPSALDGVEVPDAGFNNDLHASAEYRAHLVKVMAKRAVADLLS"
     gene            complement(5880..8228)
                     /locus_tag="AC16VR_10325"
     CDS             complement(5880..8228)
                     /locus_tag="AC16VR_10325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR6011596.1"
                     /translation="MSEAAATGIGASVRRREDVRFITGSGRFTDDLTQPGQTYAVFLR
                     SPYARARIGEIDVSEALATEGVIAVLTGRDMAADGLGDLPCGWLVKQKDGSDMISAPH
                     PPLATEVANYVGEPYGLVIAESLAAARQAAECVMADFEELDAVVDLATAVDSSQIHEG
                     VPGNRAYDWEIGDREATEAALAGAAHVTRIDLTNNRLIPNAMEPRAALGVYDPAGDDY
                     TLYTTSQNPHLERLVLSAFVQVAPEHKLRVIAPDVGGGFGSKIFIYAEETAVIWASRR
                     VGRPVKWTAERSESFLADAHGRDHITHAELGLDAEGNFTALKVQTSANLGAYLSTFGS
                     SVPTYLYGTLMAGQYRTPNIYVEVQGIYTNTAPVDAYRGAGRPEATYVVERIVETAAR
                     ELGTDPAELRRRNMIRPDQFPYETPVALVYDTGDYEAGLDRALALVDYAGFPARRAAS
                     EAAGKRRGIGLACYIEACGLAPSQLAIQLGAGVGLYESGEVRVNPTGSVTVFTGSHSH
                     GQGHETTFAQVVSDRLGVPFESVEVVHGDTGRVEFGLGTYGSRSLAVGGSALIAATDK
                     VIAKGKRIAAHMLEAQESDVEFADGNFVVRDSNQSLSFGEVAFAAYVPANYPEDLEPG
                     LSEKAFYDPENFTYPAGTHIAEVEIEEETGVVRLVNFVAVDDFGHVINPMIVDGQVHG
                     GLAQGAGQALLEHGVYDDYGQLQTGSYMDYCMPRADDVPDFVVDTTVTPCTHNPLGVK
                     GCGEAGAIGSPAAIMNAVTDALGVNDISMPATAEKVWRALRA"
     gene            complement(8238..8696)
                     /locus_tag="AC16VR_10330"
     CDS             complement(8238..8696)
                     /locus_tag="AC16VR_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019366335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR6011597.1"
                     /translation="MKVALNVNGTDRELEVPDNTLLVDLLREHLRLTGTHVGCDTSQC
                     GACTVHLNGRSVKSCTVLAAHAEGAEVVTIEGIGAPDDLHPMQEAFRDCHALQCGFCT
                     PGMIMSAIDLVNQSDDLDNQAVREGLEGNICRCTGYHNIVRAVLQAAEKM"
     gene            complement(8736..9287)
                     /locus_tag="AC16VR_10335"
     CDS             complement(8736..9287)
                     /locus_tag="AC16VR_10335"
                     /inference="COORDINATES: protein motif:HMM:NF017999.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6011598.1"
                     /translation="MEQFGEYRISAARAMVWEGLNDPEVLGRCIQGCLSMERTSDEQF
                     DASVKAKVGPVSATFQAQLMLSEIDAPSSYTLTANVKGGAAGFGKGTAKVSLLEDGAS
                     TLLRYQVSANVGGKLAQVGSRLIDAAARKMADDFFAEFGREVGGAEPEALAAVAEAPV
                     AGGRWKIWVAVAAIVVIILLLMV"
     gene            complement(9347..10957)
                     /locus_tag="AC16VR_10340"
     CDS             complement(9347..10957)
                     /locus_tag="AC16VR_10340"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spinster family MFS transporter"
                     /protein_id="HIR6011599.1"
                     /translation="MQQNEPSDSSGESADGVGGRYANYVLIVLIIVYVFNFIDRQILS
                     ILAEEIKADLGISDADIGFLYGTAFAIFYAVFGIPMARLADTWIRKDLIAIGLGFWSL
                     MTALSGTARNFASLATFRFGVGVGEASASPAAFSMLSDYFSPRVRATVLAIYSSGVYI
                     GAGIGVFLGGIIVDSWNDAFPDPVLAPFGLKAWQAAFFAVGLPGLLMAIWVWTLREPQ
                     RGVSEGLVVPTHPHPFRETARSLVTVLPPFTVVSLWVSGGARTVAVNLGLALLIAAVG
                     YGANLATGTPNQWIALGIGVYAVVSWAQGLKLSDPVAFGMIFGSRAMVLLTLGFPCLA
                     FVTYGVGFWSPPYLLRAHGVNLAEAGTLLGLGSALGGWIGVTLGGIVADRLRARSVNG
                     RIYIGVASPFLAAPAAIWFVLTDNVWVAYFCAFVFSMFSPMWLGAAASTVNDLVLPRI
                     RAVASAYYILMLTFIGLALGPYLIGQVSDIYLASGLDDGQALGRAILWGCGMLGVSLL
                     FLAGAMRYLGAEEANRLDRARALGEPMD"
     gene            complement(11050..11715)
                     /locus_tag="AC16VR_10345"
     CDS             complement(11050..11715)
                     /locus_tag="AC16VR_10345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR6011600.1"
                     /translation="MELYDVMRTTFAAREFLDEPVTDGDIRNILDNARFAPSGGNRQG
                     WRVIVIREAETREAMLPMIMPTFQRYVAQVQAGEAPWNTINETRLTAEEIAATEPPEA
                     FIRQVLAAPVLLLVLVDLSVVASFDAQLDRVGVISGASIYPFVWNVLLAARNEGFGGT
                     LTTFIAGREGEIKRLLGIPDHLAVAALLPIGRPVKQLTRLSRKPVDEFAMLERWDGAP
                     LGD"
BASE COUNT         2140 a         3932 c         3767 g         1914 t
ORIGIN      
        1 cgaagggatt gccgatggcc acgacgaagt cgcccacccg cagttcgccg gagtccgcga
       61 agccgatctg ctgcaggtcg tccgcctcca cctttagcac cgccagatcg acctggggat
      121 cgctgccgac gagctcggcc gtcatcgaac ggccgtcggc cagcgtcacc gagatctcat
      181 cggcccgttc gaccacgtga ttgttggtca cgatgaagcc cttgccggca tcgatcacca
      241 cgccagagcc cgcactctgg gttcgccggt aacggcgctg gtcagggacg ttgaagaacc
      301 ggcggaagaa cggatcctcc aacagcgggt tgcggacctg taccgtggtg taggtggcaa
      361 tgttgaccac ggcgggcgtg gcctggtcga gcatgggcgc aaggctggga agctgctcgc
      421 cgcccggcag ccccagcggc aaagccgcag cggccgtcaa gctgcccagc agcagccacc
      481 cgaaaaccgc gcacttcaga gtgtgcctca attgaccgtt cctcaccttg gttggtagca
      541 ccgatgttgg acagcgcccg gttgccggca gttccaccag gacggggcgg ccggcaggga
      601 ctcagtgtac actcactgct cgcattcggg ggagcacgga cgtgccgatc aaaaatgtct
      661 tcgaagccat ccagcgcgag aaggcgaaca tagagaactt gagcgtcgag gagctcaagc
      721 aggctcttgc agacgacccc gacctgctgc tgctcgacat tcgcgagatc caggagcgcg
      781 tggaccttgg caccataccc ggttccgttc acgtccccag gggcatgctg gagttctggg
      841 ccgacccggc tagcccgtac tttcgggact atttccagga agaccggcgc accgtggtgt
      901 tctgcgccgg cggcggccgc tccgtgctgg ccgtcagagc cctgcaggac atgggcttta
      961 ccgacgtggc ccatctcgag cccggcttcg gcggctggaa aaaagccgcc gagcccatcg
     1021 aagacgtggc ttccagcagc cgctgggtac ggcgggccga cgacgactga agccgtgccg
     1081 cttgcgggac catcacctcg ggatggtcac cggcgagcgc tcgtgccaga atcgacaacc
     1141 tctcggcggg actagctcat ctggtagagc gacagcttcc caagctgtag gcgacgggtt
     1201 cgagtcccgt gtcccgctcc atcttcaccc cgggaattcc gagcgcagat cgctgccacc
     1261 ctgctcggtc gcgaccagcg tcgccggcca catcccccgg acggagttga gcagccagat
     1321 gcgctccgcc tcgacgagca ggctaaccgg aacgcgcgct tcttcgagga ttttgtcggc
     1381 gacgagcacc tgacgttcga tcccgggaag caggccgcag ccgagctcgg gcgtcagcag
     1441 gcggccgtcg acctccacgg ccacgttggc aatggtcgac tccgtcacct gcccgtcgcc
     1501 gttgaccagc agcacatcgt cgaggtccgg ccggacgctg cgggccgcgt cgagcgcctt
     1561 gtcatacacc gaacgttgcg tcgtcttgtg gtacacgaac tcgctctgca aatcctccat
     1621 cggttcggat agggccacac ggtagggttc gggcaaaggt ttcagcggcg aagcctctag
     1681 ccttacgtga cccgaacggt cggcgagcag gcggacgcgc atgggctcgc gatacgccga
     1741 cgcggccgct tcgagcaagg cttgcgcctt tccggtgctc agcgaccagc agaaatagtc
     1801 agccgcgctg tgcagcctct cgagatgctc ctcgagacgg cgaaacccgt cacgggggtg
     1861 ccaggccagg gtttcgagca gctcgaattc ccggcgccca ccggtgacga tgcgcgcttt
     1921 cgcgaagcac tcccgatact cgtcttcggc tcgggagtcc cagacgatgc cgccacccac
     1981 accgtactcc gcctcggacg tcgtcttgtc gatggcggcg gtgcgaatag ccacgttgaa
     2041 ctggcagcgc ccctcaggac gcacgaagcc aatggtgccc gtgtagatcc cgcgcggctc
     2101 atgctcgagc tcgcgaatga tctccatggt tctgcgtttg ggcgctccgg tgatggaggc
     2161 cgccgggaac agggcgccca tgacgtccgg caggctggcg gtcgatcgtg ctcttaccgt
     2221 gctggtcatc tgccagacgg tggggtaccg ctccagggca aagaggtcgt cggcaaccac
     2281 cgatccgatc ctggcaatgc ggccaaggtc gttgcggacc atatcggtga tcatcaggtt
     2341 ttcggcacga ttctttgccg atgcggccag ccagccggcc tgatcccgat ccgtttggtc
     2401 ccgcagaccg cgcggcgcgg tgcccttcat tggccgggaa acgagctcgt ccccgtcgcg
     2461 ggcgaagaac agctcgtggg atgcgctggc aacggcgaac ccgggcgtgt cgatgaaagc
     2521 agccaggcca gggccctgag agctcaccat gttcgcgaac aaggcggcgg gggaatcgtt
     2581 cagacgagcg cgcaggcgaa acgagtagtt cacctgatag gtgtcaccag ccgcgatgta
     2641 gtctcgaatc cgggcgatgg ccgaatcgta gtccttgcga gtgacggacg ccgaccatcc
     2701 gccaaggcag tcgcccgggg caagaggaag gtcggcgctc ggcagaaccc gccggaacag
     2761 accgaaccat gcgagcggaa ggagccggtg cggcctgtgc gtcaccagct cgcgcgcaat
     2821 tcccggagcg gcttcatagg tcagaaatcc cgccgcgaag aggccgtcgc ccaccgtgcg
     2881 gctctcgatc tcggcgacga cgtcaaggac ctgatcctcg cggtccgcga tcagcagctc
     2941 aacgggatcg caaaagcgca accattgttg cgcgacggca tcgtagacta tgacgtctgt
     3001 cgacatgggc ttgcggatca gggtggccgg gtatgctaac cgcggtcagc tgcgagactc
     3061 aatgcgccat tcccggacag gcggccccta cccaccctgg gcagatgcgg taggcaccaa
     3121 agaagggtca gtcgaagtcg agcagatccg cggagatcac ctctaccgct gcccgttctc
     3181 ccagcatgaa cgcggccacc gcccgacgga agtagtcccg gggctcttct agaaacgccg
     3241 catgatccgc cccgggtatg ctcacccagg ccttgtccgc cgtggccagc cccgcaaaaa
     3301 agcgcgagag aaccttgggg tccgtgagcg ggtcgtgctc agcgtccacg agcagcgttg
     3361 gcacggtgac gcgggcggcg tccagagcca gccactccgg ataggcgcgc cagtcgacgc
     3421 gcaccggatc gtgcttgagc gcgctctcca cgaacgcatc gatcgtttcc cggcgggcag
     3481 ccccgggggc aatgaaatcc gacctggcat ccgctgcgga attggcgatc cgcgccggtt
     3541 cggtggtcag tcctggtggg ttcaccgccg agtcctgccg caaggggtag ccgaaaacaa
     3601 tgagccccga gatccgcccg ggctgctgct gagcggcgag ttgggccacc atggcccctc
     3661 gggagtatcc gaacaggtac ggcccgggga ggccggggtt gcgctgggca atccattcca
     3721 gcaccgcgat gacgtccgca gcggcccggg acggggtgag ccatccggag gcatcccggg
     3781 gcgtgtcccc gtagccgcgc atgtccagcg cgtacgtggc aataccctcc tcgaccagac
     3841 cgtccatgag agaaagatcc tcgccgggca cgttcagatc gaagttgggc cgcgcgctcc
     3901 acgtgcgccc gtgaacgagc agcacgacgc gcttggcgct ggccggcgct ttccgccata
     3961 ccgcaagcgg cacgccgtcg acgatcacgt ggtgggccga gagcggtcgc gccggctcgc
     4021 tcgagatcgc cggcagcgaa agaaccgcca gcagcgaccc caggataagc cacgtcaggg
     4081 ctgggggccg catcgtcaac ttgctcctca tggtctgcgt ccaaactccg ggtcattcgg
     4141 cgacgttgca cgccccccgg accgcgcaat ccttcgcgac tatagattca acgttctgga
     4201 acgtctaatg ttcagagcat caaaacaacg cggttcacgg gggaaacgag atggacgtct
     4261 cgggcaagat cgtcgtcgtc accggcggcg ccagcggtat cgggcgtgcc atggcgcagc
     4321 gctttcatgc cgagggcgca ggcaaggtga tcgtcgccga cctcgacggc gacggcgccc
     4381 gccgcgtggc cgacgaaata gatggttcgt ccatgcaggt agacgtcggc agggaagcag
     4441 acctcgtcgc gctgatcgag cacgctgaat ccgagttcgg gccgatcgat ctgttctgtt
     4501 ccaacgcggg aatcgggatc ggcaaaggtc tcgacgagcc cgacgaggtg tgggacacca
     4561 tctggaaggt caacaccatg tcccacgtct acgccgctcg ccacatggtg ccgcgcatga
     4621 aggcgcgcgg aggcggttat ctgctcaata ccgcctcggc cgcgggcctg ctgacacaga
     4681 tcggctcggt cacctacgcg gtgaccaagc acgccgcggt tgcgctggcc gagtggatcg
     4741 cgatcactca cgctcgcgac ggcataaaag tctccgtgct ctgcccccag gccgtgcgca
     4801 ccccgatgac ggccgatggc ccgggcgtgg ctggcgtgga cggcatgatc gaacccgagg
     4861 aggccgccga atgcgtgatc gaagcgctgc gcgaagagcg cttcctggtg ctgccgcatc
     4921 cggaggtcgc cacctacatc cagcgcaaga cgtccgatta cgaccgctgg ctgaacggca
     4981 tgcgccgcct tcaggatctg tacgtcgccg aagaaggcga gtgagcgctg ccgccgacgc
     5041 cgccggctga gccagccgcc ccggtacgaa gcttcagctc agcaggtccg ccacggcccg
     5101 cttcgccatc accttgacca ggtgcgcccg gtactcggcg gaggcgtgca ggtcgttgtt
     5161 gaatcccgca tccgggacct cgacgccgtc cagcgccgac gggtcgagat tgcggctgag
     5221 caactcctcg atagcagtgg cgcgaaaggc gcagggaccc gcaccggtca cccccacgcg
     5281 gaccttgccg ccgaagtcgg ccaccagcac gcccaccacg gcgtagcggg aagcagggtt
     5341 cgggaacttg cggtaggcgg cccgggtcgg cacgggaaag tcgatccgcg taatcagctc
     5401 tccggggttg agggccgtct cgaagagatc tttgaagtag tcgtccgctt cgatgctgcg
     5461 cgtctgagta tgcaccgtgg cgcccagacc gatcagcgca gcggggtaat cggcggccgg
     5521 gtcgctgttg gcgacggatc cccccagcgt gcctcggttg cgcacctggc cgtcgccgat
     5581 ctggccagcc aggaaagcca gcacgggaag ggtcgcgcga atctccggcg agctcgccac
     5641 cgcatcgtga acggtgaacg cgccgatgct gacccggtcg gcgctcacct cgataccgct
     5701 gagcgcttcg atacccgaca ggtcgatgac gtcggtaggc gcggccagcc gctgcttcat
     5761 cgtcgggatg agcgtctgcc ccccggccat gtagatcccg tcgtccatag attcgaacag
     5821 cccgacggct tcctctaccg acgccgggcg gtggtagttg aactgataca tggcttctcc
     5881 taggcccgca gcgcccgcca caccttctcg gccgtggctg gcatggaaat gtcgttgacg
     5941 ccgagggcgt cggtgacggc gttcatgatc gccgccgggg agccgatggc gcccgcttcc
     6001 ccgcagccct tgacgccgag agggttgtgg gtgcacggcg tcaccgtggt gtcgaccacg
     6061 aagtccggca cgtcgtccgc gcgcggcatg cagtagtcca tgtagctgcc tgtctgcagc
     6121 tggccatagt cgtcgtacac gccgtgctcc agcagcgcct gtccggcacc ctgagcaagc
     6181 cctccgtgaa cctgcccgtc gacgatcatc ggattgatga cgtgcccgaa gtcgtcgacg
     6241 gcgacgaagt tgacgagccg gacgacgccc gtctcctctt cgatctccac ctccgcaatg
     6301 tgcgttcccg ccgggtaagt gaagttctcg ggatcgtaga aagccttctc ggaaagtccc
     6361 ggctcgagat cctcggggta attggccggc acgtacgccg caaaggcaac ctccccgaag
     6421 gacagcgact ggttgctgtc cctgacgacg aaattgccgt cggcgaactc cacgtcgctc
     6481 tcctgggctt cgagcatgtg cgcggcgatg cgtttaccct tggcaatgac cttgtccgtg
     6541 gccgcgatca acgccgaacc accaaccgcc aacgacctcg acccgtaggt accgaggccg
     6601 aactcgacgc ggccggtgtc gccatgcacc acctcgacgc tctcaaacgg cacgccgagc
     6661 cggtcagaca ccacctgggc aaaggtggtc tcgtgaccct ggccgtggct gtgagatccg
     6721 gtgaagacgg tgacggaacc cgtcgggttc accctcacct cgccgctctc gtagagcccg
     6781 acgcctgcgc cgagctggat ggccagctgc gagggcgcga ggccgcaggc ttcgatgtag
     6841 cacgcaaggc caatgccacg ccgcttgcct gccgcttcgg aagcagcgcg gcgagccggg
     6901 aagccggcgt agtcgacgag cgcaagcgct ctgtccagac cggcttcgta gtcgccggtg
     6961 tcgtagacca gcgccaccgg cgtctcgtag gggaactgat ccggccggat catgttgcgc
     7021 cgccgcagct ccgccggatc cgtgccgagc tccctggcgg cagtctcaac gatccgctcg
     7081 accacatagg tggcctccgg acgcccggct ccgcggtagg cgtctaccgg tgcggtgttg
     7141 gtgtagatgc cctgcacctc gacgtagatg ttcggggtcc ggtactggcc ggccatcagc
     7201 gtcccgtaga gataggtcgg gacggaggag ccgaacgtgg acagataggc gccgagattc
     7261 gcgctggtct gcactttcag cgccgtgaag ttaccttccg cgtccaggcc aagctccgca
     7321 tgggtgatgt gatcccgacc gtgggcgtcg gcgagaaacg actcgctgcg ctccgccgtc
     7381 cacttcaccg gccgaccgac cctgcgcgat gcccagatga cggcggtctc ctccgcgtag
     7441 atgaatatct tggaaccgaa accaccgccc acgtccgggg cgatgaccct cagcttgtgc
     7501 tccggtgcga cctgaacgaa ggcggacaac accagccgct cgaggtgggg gttctggctg
     7561 gtggtgtaga gcgtgtaatc gtcgccggcc ggatcgtata cgcccagagc ggcgcgaggc
     7621 tccatggcgt taggtatcag gcggttgttg gtcagatcga tccgggtgac gtgggcggct
     7681 ccggccagcg ccgcttccgt ggcctcccgg tcgccgatct cccaatcgta ggcccggttc
     7741 ccgggcacgc cctcgtgaat ctggctggaa tcgacggcgg tcgccaggtc gactaccgcg
     7801 tccagctcct cgaaatccgc catgacgcac tcagcagcct gcctggcggc ggccagtgac
     7861 tccgcgatca ccaatccgta gggttcgccc acgtagttgg cgacttcggt ggccagcggc
     7921 gggtgcggcg cggagatcat gtccgagccg tccttctgct tgacgagcca cccgcaggga
     7981 aggtcaccca ggccgtcggc tgccatatcc cggccggtga gcacggcgat cacgccctca
     8041 gtggcaagcg cttcgctcac gtcgatctcg ccgatcctgg cgcgcgcgta ggggctgcgc
     8101 aggaatacgg cataggtctg gcccggctgc gtcaggtcgt ccgtaaagcg cccgctgccg
     8161 gttatgaaac ggacgtcctc gcgacgcctg accgacgctc cgatccccgt tgccgctgct
     8221 tcactcatct cgaatcctca catcttctcg gctgcttgca gcacggcacg cacgatgttg
     8281 tggtaacccg tgcaccggca gatgttgcct tccaggcctt cccggaccgc ctggttatcc
     8341 aggtcgtccg actgattcac cagatcgatg gcgctcatga tcatccccgg cgtgcagaac
     8401 ccgcactgaa gcgcgtggca gtcgcggaac gcctcctgca tggggtgcag gtcgtctggc
     8461 gcgccgattc cctcgatggt caccacctcg gcgccttcgg cgtgtgcggc gagcaccgtg
     8521 cacgacttga ccgagcggcc attgagatgc acggtgcacg cgccacactg gctcgtatcg
     8581 cagccgacgt gggtgcccgt gagcctgagg tgctcccgca ggaggtccac caggagcgtg
     8641 ttgtccggca cctcgagttc gcggtcggta ccattgacgt tcagcgcgac tttcatattc
     8701 gaatcctaga ctctttgatt cacgacgacg gccgctcaga ccatgagcag cagtatgatc
     8761 accacaatgg ccgcaacggc cacccatatc ttccagcgcc cgccggcaac aggcgcctct
     8821 gccacagccg ccagagcctc gggctccgca ccgcccacct cgcgtccgaa ctcggcaaag
     8881 aaatcgtctg ccatctttcg ggctgccgcg tcgatcagcc gggatcccac ctgagcgagc
     8941 ttgccaccga cgttggcgct cacctgatag cggagcaacg tgctggcacc gtcttctaag
     9001 aggctgacct tcgccgtccc tttcccgaac ccggcggcac ctcccttgac gttggccgtc
     9061 aacgtgtagc tggagggcgc gtcgatctcg ctgagcatga gctgcgcctg aaaggtagcg
     9121 ctgaccggac ccaccttggc cttgaccgag gcatcgaact gctcatccga cgtccgctcc
     9181 atggacaggc agccctggat gcagcgcccg agcacctcgg ggtcgttcag gccttcccat
     9241 accattgccc gtgcggcgga aatgcgatat tcgccgaact gctccactcg gaacctcctc
     9301 cctcgagcgc ctaatttcgc acgattgcct tatcccggca acgcgttcag tccatgggct
     9361 ctcccagggc ccgcgcgcgg tcgagccggt tggcctcctc ggctccgagg tacctcatgg
     9421 cgccggcgag gaacaggagg ctgacgccca gcatgccgca gccccagaga atcgccctgc
     9481 ccagcgcctg gccatcatca aggcccgacg ccagatagat gtcgctcacc tggccgatca
     9541 gatagggccc gagggcgagc ccgatgaagg taagcatcag gatgtagtag gcggacgcca
     9601 ccgcccttat tcgcggcagg acaagatcat tcaccgtgct cgctgcggca ccaagccaca
     9661 tgggcgagaa catgctgaac acaaacgcgc agaaataggc cacccagacg ttgtcggtca
     9721 gcacgaacca gatggccgcg ggtgcggcga gaaagggcga agccacgcca atgtatatgc
     9781 ggccgttcac cgaacgcgcc cgcagccggt ccgccacgat gccgcccagc gtgacgccta
     9841 tccagccgcc cagggcggaa cccaagccca gcagcgtgcc ggcctcggcg agattgacgc
     9901 catgggcgcg aagcagatag ggcgggctcc agaaacccac gccgtaggtc acgaaagcca
     9961 ggcaggggaa gcccagggtc agcagcacca tggcacggga gccgaagatc atgccgaacg
    10021 ccacaggatc cgaaagcttc aggccctggg cccacgagac caccgcgtag acgccgattc
    10081 ccagagcgat ccattgattc ggcgtgcccg tcgccaggtt cgcaccgtag ccgacggccg
    10141 caatgagcag ggcgagtccg agattgacgg cgacggtccg ggcgccgccg ctgacccata
    10201 gcgagaccac ggtgaacggg ggaagcacgg tgacgagcga ccgggccgtt tcgcggaaag
    10261 gatgcgggtg cgtcggcacc acaagcccct cgctgacccc gcgctgcggc tcgcgcagcg
    10321 tccagaccca gatggccatg agcaggccgg gtaggcctac cgcaaagaag gccgcctgcc
    10381 aggctttgag gccgaacggc gccaggacgg gatccggaaa ggcgtcattc caggaatcga
    10441 cgatgatccc accgaggaag acgccgatac cggcgccgat gtagacccca ctggaataga
    10501 tggcaagaac ggtggcccgc acgcgcggcg agaagtaatc ggagagcatg gagaaagcgg
    10561 ccggcgacgc gctcgcctca cccacgccga ccccgaagcg gaaagtggca agggaagcaa
    10621 aattccgggc agtccccgac agcgccgtca tcagactcca gaagccgaga ccgatagcga
    10681 tgagatcctt gcggatccag gtgtccgcca gccgagccat gggaatgccg aacacggcgt
    10741 agaagatggc aaaagcggtt ccgtaaagga aaccgatgtc cgcatcggat atgcccaggt
    10801 cggccttaat ttcctcggcg aggatggaca ggatctgccg gtcgatgaag ttgaagacgt
    10861 aaacgatgat gagaacgatg agaacgtagt tcgcatacct accgcccacc ccatcggccg
    10921 attccccgga agaatcgctc ggttcgttct gctgcacggc tctcccctcg acccggtggc
    10981 atccccttgt gcgctgggca gcctagcaaa ccacgaagcg atgtccatgg ttaggcggcg
    11041 cgggcgcctt caatcgccca gcggcgcgcc gtcccagcgt tccagcatcg cgaactcgtc
    11101 caccggcttg cggctgagcc gggtcagctg cttgaccggc cgcccgatcg ggaggagggc
    11161 cgccacggcg aggtggtcgg gtattccgag aagacgcttg atctccccct cgcgcccggc
    11221 gatgaaagtg gtcaacgtac cgccgaatcc ctcgttcctt gcggccagga ggacgttcca
    11281 gacgaacggg tagatggagg cgccggaaat caccccgacc cggtcgagct gcgcgtcgaa
    11341 ggacgccacc acgctcagat ccacgagaac cagcagcagc accggcgcgg caagaacctg
    11401 ccgaatgaag gcttccggcg gctcggtagc ggcgatctct tcagccgtta ggcgggtctc
    11461 gttgatggtg ttccacggcg cttcgcccgc ctgaacctgt gcgacgtagc gctgaaacgt
    11521 gggcatgatc atgggcagca tggcttcccg ggtctccgct tcgcggatca cgatcacccg
    11581 ccaaccctga cgattacccc cgctgggggc aaaccgcgca ttatctaata tattgcgtat
    11641 atccccgtct gtgaccggtt cgtccaggaa ttcccgggcc gcgaaggtcg ttctcatcac
    11701 gtcgtagagt tccacctgat ctcccaattt ataacagttg cttgacagcg cac
//
LOCUS       DBNHQR010000457         4034 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_486488, whole genome shotgun sequence.
ACCESSION   DBNHQR010000457 DBNHQR010000000
VERSION     DBNHQR010000457.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4034)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_486488"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="AC16VR_10350"
     CDS             complement(<1..802)
                     /locus_tag="AC16VR_10350"
                     /inference="COORDINATES: protein motif:HMM:NF019845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR6011601.1"
                     /translation="MKAALMENGRIWVDTVPDPEPRLGEVLVKSAGCGICGSDLHAYR
                     HTEEFVATSRESGGAFRLTTMNPVVLGHEFCAEVVDYGPGTQRRLPVGTLVCAIPALP
                     RGGGFEPIGYSEDAPGGFAEYFLLNERLMMPVPPGLSWELAALTEPMAVGHHAINKAR
                     LDGEAVLVIGAGPVGLAVIANLKQRGVGPVVVAELSPGRRALAERLGADVVVDPAAGS
                     PYAVPEVSRAKGLVIFECVGVPGMIGQIFLDAPREAKIVVVGVCLQQDV"
     gene            complement(824..2116)
                     /locus_tag="AC16VR_10355"
     CDS             complement(824..2116)
                     /locus_tag="AC16VR_10355"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6011602.1"
                     /translation="MASNPQSGDVSDIQLFDVETLKDPWDAYARLRDHAPVFFVPGFD
                     IHVVTRYDLVREVIKDTETYSSKFDLFLNRSRELMFASAPPEVQAELIRLNDEMIQIP
                     PTMLTLDEPEHTKYRSLVSQLFTVSQIRKAEDAVQAVIDETLETFVDRRSVDFMDAFA
                     FPVPLRIIADRLGIPEDDRAFFDDAATAAAAGLRLSPITPEEMVRRAQLGVDLQKLLV
                     RIIEERRTDPREDMISILANSKLEAEDRYLTHGECLSILNQFLVAGHETTTSTFGWGM
                     LLLCRNPEAQRALRADPSLTRTFVEEALRLEAPVQGLPRLVTKDTELGGVELEAGKLI
                     MIRYGAANRDGRQFENPDELDLHRKKAGMQLAFGSGVHHCIGAPLARQELNLGFPTLL
                     RHMSEIRLSADHPPPEAEASFILRNLPELHIDYDLIEP"
     gene            2258..3811
                     /locus_tag="AC16VR_10360"
     CDS             2258..3811
                     /locus_tag="AC16VR_10360"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR6011603.1"
                     /translation="MTDPLTELARSQPDKPALIDDRPDGTLLKWTYSELEERTNALAR
                     VFQNAGVEAGNRLIWCGPNSAWVVAAGIAARKIGAVAVPLNYRLTAEEAAYVVDNSDA
                     VTVFADAGYADLFAEIRDDIPQVREVLIFDGPAGPGQTSAEERLAQEGTEELAVKVDP
                     TTSGTMIYTSGTTGRPKGAVRKDTGDPALIAALMELMQLRGDDVYLTTGPLYHSGPSG
                     FMAFAFAFGNTIVLQRKFDAEDWLRLIDKYGVSFTFSAPTPVRLATQLPPETIKRYDL
                     SSVRITIANAAPWSFALKKAYLEHFPADSLFEVYGSTELGVNTIMLPADQLRKPGSCG
                     QAAPGVEIILIDEEGREVTEPKVPGEVFIRSPSVFSTYHKAEDKYLEDRRGDMQTVGD
                     IAYRDDEGFYYICDRKKDMIISGGVNVYPAEIEAALEHHPDILDVAVFGIPSEEWGES
                     VHAVVVKRSGSGLNEEAIQAFAREHLAGYKVPRSIEYRDAIPRTGTGKILKRELRAPW
                     WEGHNSSVS"
BASE COUNT          767 a         1308 c         1298 g          661 t
ORIGIN      
        1 tgacgtcctg ctgaaggcag acgcctacca ccacgatctt ggcttcccgc ggagcgtcga
       61 ggaatatctg cccgatcatg cccggcacgc ccacgcactc gaagatcacc aggcccttcg
      121 cgcgggagac ctccgggacc gcgtaaggcg agcccgcggc gggatccacc acgacgtcgg
      181 cccccaggcg ctcggccaga gctcgccgac ccggtgagag ctcggcgacc accaccggcc
      241 cgacgcccct ctgcttgagg tttgcgatca ctgcaagacc tacgggaccg gcgccgatga
      301 cgagaaccgc ttcgccatcg agccttgctt tgttgatggc gtggtggccg acggccatgg
      361 gctccgtcag cgcggcaagc tcccaggaaa gcccaggggg tacgggcatc atcaatcgct
      421 cgttgagcag aaagtattcg gcgaaccccc ccggcgcgtc ctccgagtaa ccgatgggct
      481 cgaaaccgcc gccccgcgga agcgcgggaa tggcacagac caaggtgcca acgggaaggc
      541 ggcgctgcgt cccgggtccg tagtcaacga cctcagcgca gaactcgtgg cccaggacca
      601 ccggattcat tgtggtcagg cggaaagcgc ccccggactc gcggctggtc gccacgaact
      661 cctccgtgtg acggtaggca tggaggtcag atccgcatat cccgcaaccc gcggacttga
      721 ccagcacctc tccgagtcga ggctcgggat cggggacggt gtctacccag atccgaccat
      781 tttccatgag cgccgctttc atgatgatcc ggcgtctcgg cgatcagggc tcgatcaagt
      841 cgtagtcgat gtgcagctcc ggcaggttgc ggagaatgaa gctggcctcc gcctccgggg
      901 gcggatgatc ggccgataga cgaatctcgc tcatgtgccg tagcagggtc ggaaaaccca
      961 gattcagctc ctgccgggcc agcggggcgc cgatgcagtg atgaaccccg gaaccgaacg
     1021 ccagctgcat gcccgctttc ttgcggtgca ggtcgagctc gtccggattc tcgaactgac
     1081 gaccgtcgcg attggccgca ccgtagcgga tcatgatcag cttgcctgcc tccagctcga
     1141 cgccgccgag ttcggtatcc ttcgtcacca ggcgcgggag cccctggacg ggtgcttcca
     1201 gccgcagggc ctcctccacg aaggtccgcg tgagtgacgg atcggcgcgc agcgcccgct
     1261 gggcctcggg gttgcggcac agcagcagca ttccccagcc gaacgtgctc gtcgtggttt
     1321 cgtgtcccgc cacgagaaac tggttgagga tgctcagaca ctcgccatga gtgaggtaac
     1381 gatcctcggc ctccagcttg ctgttcgcga ggatgctgat catgtcctcg cgagggtcgg
     1441 tccgtcgctc ttcgattatc cggaccagca gcttctgcag atccaccccc agctgggccc
     1501 ggcggaccat ttcttcgggc gtgatggggc tgagccggag accggccgcc gcggcggtgg
     1561 ccgcgtcgtc gaagaacgct cggtcgtcct cgggaatacc cagccgatcg gcgatgatcc
     1621 tcagcggcac gggaaacgca aacgcgtcca tgaagtccac cgagcggcgg tcaacgaacg
     1681 tttccagcgt ctcgtcgatg acggcctgaa cggcgtcctc ggccttgcgg atctgactga
     1741 cggtgaacag ctgcgatacc agcgaccgat acttcgtatg ctcgggctcg tcgagcgtga
     1801 gcatcgtggg tgggatctgg atcatctcgt cgttgagacg gatgagctcc gcctgcacct
     1861 cgggcggtgc cgatgcaaac atcagctccc gggaacggtt gaggaacaag tcgaacttgc
     1921 tcgagtaggt ttccgtgtcc ttgatgacct cgcgcacgag gtcgtatcgc gtcaccacat
     1981 ggatatcaaa accaggtacg aagaaaacgg gggcgtgatc gcgcaggcgg gcataggcat
     2041 cccaggggtc cttgagggtt tctacgtcga acagctggat gtcagagaca tccccagatt
     2101 gcggattgct ggccataagc acactccccg aagttcgttc gttcccggag aattctacgc
     2161 ctgctgcccg tcggttacga atggctgcgg atgcacgacc acgaacgccc ggctaccatc
     2221 agcccgggaa actcattcgg ggcaagagga gagcgagatg acggatccgt tgaccgagct
     2281 ggcgcgcagc cagccggata agccagcgct gatcgacgac cggcccgacg gaaccctgct
     2341 gaagtggacg tactcagagc tggaagagcg cacgaacgcc ctcgcacggg tctttcagaa
     2401 cgccggcgtg gaagccggca accggttgat ctggtgcggg cccaattctg catgggtggt
     2461 cgcggccggc atcgccgcgc gcaagatcgg cgcagtcgcc gtgccgctga actaccggct
     2521 gaccgcagag gaagccgcct acgtggtgga taactccgac gccgtgacgg ttttcgcaga
     2581 tgccgggtac gccgacctgt tcgccgagat tcgggacgac atcccccagg ttcgggaggt
     2641 cctgatattc gacggtccgg ctggaccggg acagacgtcg gccgaggagc gcctggctca
     2701 agagggcacc gaggaactcg cagtgaaagt cgacccgacg acctccggca ccatgatcta
     2761 cacctccggg accaccggtc gtccgaaagg cgcggtgcgc aaggacaccg gcgatccggc
     2821 cctgattgcc gcgctgatgg aactgatgca gctcaggggc gacgacgtct acctgaccac
     2881 cgggccgctc tatcactccg gacccagcgg cttcatggcg tttgcgttcg ccttcggcaa
     2941 caccatcgtc ctgcagcgca aattcgacgc cgaggactgg ctgcgactca tcgacaagta
     3001 tggggtctcc ttcaccttca gcgcgccgac gcccgtaagg ctagccacgc agttgccgcc
     3061 cgagaccatc aagcgctatg acctctcgag cgtgcgcatc accatcgcca acgcggctcc
     3121 gtggagcttc gcgctgaaaa aggcctacct cgagcacttt ccggccgaca gcctgttcga
     3181 ggtttacggg tccaccgagc ttggcgtgaa caccatcatg ctgcccgcgg accagctcag
     3241 aaagccgggg tcctgcggtc aggccgcgcc cggtgtggag atcattctca tcgacgagga
     3301 aggccgggag gtcacggaac caaaagtccc cggcgaggtg ttcatccgct cgccgagcgt
     3361 gttctccacc tatcacaagg cagaggacaa gtatctggaa gaccgccgcg gcgacatgca
     3421 gacggtgggc gacatcgcct atcgcgacga cgaaggcttc tactacatct gtgatcgcaa
     3481 gaaggacatg atcatctccg gcggggtcaa cgtctatccg gcggagatcg aagccgcgct
     3541 ggagcatcac cccgacatac tcgacgtcgc cgtgttcgga atccccagcg aggagtgggg
     3601 agagagcgtg cacgccgtcg tcgtgaaacg cagcggctct ggattgaacg aggaggccat
     3661 tcaggcattc gcccgggagc acctggcggg ctacaaggtt ccgcgctcga tcgaatatcg
     3721 cgacgcgatc cctcgcacgg gaaccggcaa gatcctcaag cgggagctgc gcgcgccctg
     3781 gtgggaaggg cacaacagca gcgtttcgta gaaacgcagc ccggtgacgt gaagaccatc
     3841 gcgcggcggg tgccccaagg cgaaagaggc gaacgaccct gcaccggaag cggcccaggc
     3901 gcggccggcg ctaagcctga tcctcccggc tgcgcagatg gcgaacgagc tgggcgtcgt
     3961 aacggctggt ggcagagtgc cggcccgtgc ggaccgccgg agcccaacgc gccccgctgc
     4021 cttccggccg gtct
//
LOCUS       DBNHQR010000458         3083 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1563513, whole genome shotgun sequence.
ACCESSION   DBNHQR010000458 DBNHQR010000000
VERSION     DBNHQR010000458.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1563513"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..163
                     /locus_tag="AC16VR_10365"
     CDS             8..163
                     /locus_tag="AC16VR_10365"
                     /inference="COORDINATES: protein motif:HMM:NF016064.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HIR6011604.1"
                     /translation="MLLLFGARLAGGCTSGHMISGISQLAVGSFIFGVMTFGSAILMA
                     KFLYRAR"
     gene            177..710
                     /locus_tag="AC16VR_10370"
     CDS             177..710
                     /locus_tag="AC16VR_10370"
                     /inference="COORDINATES: protein motif:HMM:NF016064.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HIR6011605.1"
                     /translation="MNTLIIGFLIGCAFGAILYMGGASSYRRILGTLLLKDMWIIKLM
                     ATAIGVGTLGVYLLDLGGLAHMSIKPAYIWGIALGGIIFGIGWAVSGYCPGTCVVGTS
                     EGKRDALFTLLGAFVGAFVFSLVFPYLESGLIERANFGSITLEDVLGFNGIYLAIPFS
                     LALIALAFFGLRDRYGQ"
     gene            complement(747..1220)
                     /locus_tag="AC16VR_10375"
     CDS             complement(747..1220)
                     /locus_tag="AC16VR_10375"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HIR6011606.1"
                     /translation="MQRSNPIPQAGLLALASLLASLPWADALAEGMNDEKPFAEAHVI
                     LQVSDADPSRHQTVLDIANNLTRHYGGPDMADIEIIAFSSGVKILLTTDNPHTQRIAN
                     LMENGVRFYVCGNTLDTIERRDGKRPVVLPGVREVQTGVAFMIEEIAKGYVPVHP"
     gene            complement(1245..2993)
                     /gene="soxB"
                     /locus_tag="AC16VR_10380"
     CDS             complement(1245..2993)
                     /gene="soxB"
                     /locus_tag="AC16VR_10380"
                     /inference="COORDINATES: protein motif:HMM:TIGR04486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfohydrolase SoxB"
                     /protein_id="HIR6011607.1"
                     /translation="MNRREFLEILGAASITAQLSGCTSLRTARPDDYYALPAWGDARL
                     LHITDTHAQLNPIYFREPSINLGVGAARGQLPHLVGRNLLDVLTSTNPRDVHAFTCID
                     FSEAAERYGRVGGFAYVKTLIDTLRGESGALLLDGGDTWQGSGTSLWTRGRDMVEACN
                     LLGVDVMTGHWEFTYQAQEVLDNADLLSADFVSQNVRATEDSLFEGTHVFDEDTGHVF
                     PPYVVKELNGRRIAIIGQSFPYTPIANPQRFIPDWTFGIRESDLAELVAQVRSRERVD
                     AVVLLSHNGMDVDLKLAGRVPGIDFILGGHTHDGVPVPAVVANPGGRTVVTNAGSNGK
                     FVGVLDIKFGAAGIDGYRYRLLPVFANLLEPDPQMQAHIDRVREPYLQRLEEPLAVTD
                     SLLYRRGNFNGTFDQVICDALRQVLDAQISLSPGFRWGTSVLPGQTITMENVMDQTGM
                     TYPETYVREMRGEEIRVILESVADNLFNPDPYYQQGGDMVRVGGLRYSCAPDAPNGER
                     IRNLELTDGTPLQAGAAYKVAGWATVGERSPGPPVWDVVADYLRDQGTIRIDKANEPT
                     LEGVKDDPGIARYDCS"
BASE COUNT          493 a          941 c         1034 g          615 t
ORIGIN      
        1 cggcggcgtg ctgctgttgt tcggggcgcg gcttgcagga gggtgcacca gcggccacat
       61 gatctccggc atctcgcagc tggcggtggg gtcgttcatc ttcggggtca tgaccttcgg
      121 ttccgccatt ctgatggcca agtttctcta ccgggcgcgc tgaggaggat cagcaaatga
      181 acacattgat catcggtttc ctcatcggtt gtgctttcgg cgcgattctt tacatgggcg
      241 gtgccagcag ctatcggcgg atcctgggca ctctgctgct caaggacatg tggatcatca
      301 agctcatggc tacggccatt ggcgtcggca cgctgggcgt ctacctgctc gacctcggcg
      361 gtctcgccca catgtcgatc aagcctgcct acatctgggg gatcgccctg ggaggcatca
      421 tcttcgggat cggttgggcg gtctccgggt attgcccggg tacctgtgtg gtgggcacgt
      481 cagagggcaa acgcgacgcg ctgttcaccc tgctcggcgc cttcgtgggc gctttcgtgt
      541 tctccctggt ctttccttac ctggagtccg ggctcatcga acgagccaat ttcggcagca
      601 tcaccctcga ggacgtgctc ggtttcaacg ggatttacct ggcgatcccg ttcagtcttg
      661 cgctgatcgc cctggccttc ttcgggttgc gggaccgcta cgggcagtga cggaagggcg
      721 ccgccacagc ggcgccgccc gacccttcaa gggtgcacgg gcacgtagcc cttagcgatc
      781 tcttcgatca tgaaagcgac gccggtctgt acctctctca cgccgggcag aacgaccggc
      841 cgcttgccgt cgcgacgctc gatggtgtcc agggtgtttc cacagacgta gaaacgcacg
      901 ccgttctcca tgaggttggc gatccgctgc gtgtgcggat tgtccgtcgt cagaagtatc
      961 ttgacgcctg agctgaaggc gatgatctcg atgtccgcca tgtcgggacc gccgtagtgc
     1021 cgggtgaggt tgttggctat gtccagcacc gtctggtggc gcgaaggatc cgcgtcgctg
     1081 acctgaagga tgacgtgtgc ctcggcgaat ggcttttcgt cgttcatgcc ttctgccagg
     1141 gcgtcagccc atggcaggct cgccaggagg cttgccagcg cgagcaggcc ggcttggggg
     1201 atagggttgg atctctgcat gggtctccca ggacggatcg aatctcagga gcaatcgtac
     1261 cgcgcgatgc cgggatcgtc tttcacgccc tcgagcgtcg gctcgttggc cttgtcgatg
     1321 cggatggtgc cctggtcgcg caggtaatcg gcgaccacgt cccagacggg cggccccgga
     1381 gagcgctcgc cgacggtggc ccagccggcc actttgtagg cggccccggc ctgcagcgga
     1441 gtgccgtcgg tgagttcgag gttcctgatc cgttccccgt tcggcgcgtc cggcgcgcag
     1501 ctgtagcgca gcccgccgac ccgcaccatg tcgccaccct gctggtagta gggatccgga
     1561 ttgaagaggt tgtctgccac gctttccaag attacgcgga tctcttctcc gcgcatttcc
     1621 cggacgtagg tctccggata ggtcatcccg gtctggtcca tcacgttctc catcgtgatg
     1681 gtctggcccg gcagcacgga cgttccccac cgaaaacccg gggaaaggct gatctgcgca
     1741 tcgagaacct ggcgcagggc gtcgcagatc acctgatcga acgtgccgtt gaagttgccg
     1801 cgtcggtata aaagagaatc ggtgaccgcg aggggctcct ccaggcgctg cagatacggc
     1861 tcgcgcaccc gatcgatgtg ggcctgcatc tgggggtcgg gctcgagaag gttcgcaaac
     1921 acgggcagca gccggtagcg gtaaccgtcg atgccggccg cgccgaactt gatgtccagc
     1981 acgccaacga actttccgtt cgacccggcg ttggtcacca ccgttcttcc acccgggttg
     2041 gcgacgaccg ccggtacggg gacgccatcg tgagtgtggc caccgagtat gaagtcgatt
     2101 ccgggcacgc ggccggccag cttcaggtcc acgtccatgc cgttgtggga gagcaggacc
     2161 actgcatcca cccgctcccg ggagcgaacc tgcgccacga gctccgccag atctgattcc
     2221 cgaataccga aggtccagtc cgggatgaat cgctgggggt tggcgatggg ggtatagggg
     2281 aaggactggc cgatgatggc gatccggcgg ccgtttagct ccttgaccac gtacggcggg
     2341 aagacgtgtc cggtatcctc atcgaatacg tgggttcctt cgaagagtga gtcctccgtg
     2401 gcccggacgt tctgcgaaac gaagtcggcg ctgagcagat ctgcgttgtc gagcacttcc
     2461 tgggcctggt aggtgaactc ccagtgtccg gtcatcacgt cgacgccgag aaggttgcag
     2521 gcttccacca tgtcccgtcc gcgggtccaa agcgacgttc cggatccctg ccaggtgtcg
     2581 ccgccatcga gaagcagcgc cccggactcg ccgcgcagcg tgtcgatcag cgtcttcacg
     2641 taggcgaaac caccgacccg accatagcgc tcggcggcct cggaaaagtc gatgcaggtg
     2701 aacgcgtgaa cgtcgcgggg gttcgtcgaa gtaagcacgt cgagcaggtt gcgacccacc
     2761 aggtgaggca gctggccgcg cgctgcgccc acgccgaggt tgatgctggg ttcccggaag
     2821 tagatgggat tgagctgtgc gtgggtgtcg gtgatgtgca gcaggcgggc gtcgccccag
     2881 gccgggagcg cgtagtaatc gtcgggccgt gcggtgcgaa gcgacgtgca gccggaaagc
     2941 tgagcggtga ttgacgccgc gccgaggatc tccaggaatt ctcggcggtt cattgggctg
     3001 ggggcttggg aaagcggtcc tgccaggcgc cttcttcggt gcgcgcctgc gccagagatg
     3061 cctcggccag acggagcgct ttc
//
LOCUS       DBNHQR010000459         8222 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_423173, whole genome shotgun sequence.
ACCESSION   DBNHQR010000459 DBNHQR010000000
VERSION     DBNHQR010000459.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8222)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8222)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8222
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_423173"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2226
                     /locus_tag="AC16VR_10385"
     CDS             <1..2226
                     /locus_tag="AC16VR_10385"
                     /inference="COORDINATES: protein motif:HMM:NF037144.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR6011608.1"
                     /translation="ERLESLPPTARQSALRWLQDFSFPAADAAHMSADDAGGIYYVDE
                     APDGVEEAEGAAAEPPTSGALSAAETFALHSKPGASKVLFLDFDGHVIEGTAWNANSS
                     RPDTYYARPFDTDGSPSSFGSAELETIHEVWHRVAEDFAAFDVDVTTEDPGSFGPSVG
                     HVLVTKDDDELGNAMPSAGAGGVAYVGVFGRSNYTYYQPALVYYDNLGNGHPPYVAEA
                     SSHEFGHNLSLSHDGTSTTSYFQGLGSTSSFVSWAPIMGVGYYRQVTQWSNGEYADAN
                     NTQDDLSIITGHLGPRGDDHGDDILSASLLVTDGAGTISHSNPQSDPGNFNPENKGII
                     DQQGDVDVFGLQVGTGTVDITVTPAWDAFTRSMRRGANLDIQARLLDDTGTVIATSDP
                     GDDTDASIQVQLDAGYYYLAVTGVGNSSVPYSDYASQGMYFISGQAVTGEPNGAPTAN
                     GDTAGTAEDFAVDVAVLANDSDPDGDALSILSVSSPANGTTDISGTMVIYTPNANFAG
                     NDAFTYSVGDGRGGSDSATVTVTVTGMNDAPSAVNDAAATASDTPVSVAVLSNDTDVD
                     GDALSVVGVGAAGNGTVSHDGSAVQYTPAAGFSGTDTFGYTVSDGQATASATVTVTVS
                     APAPATPGSFAATDGANGTAALTWTDSANETGYEVERESLHRKRGVWVGTSLVATPGQ
                     DATSHTDASGTGNYRYRIRAVNGAGASSWTPWQEVAVTETSGSGGGGGGTKGGGNGRK
                     K"
     gene            complement(2233..3666)
                     /locus_tag="AC16VR_10390"
     CDS             complement(2233..3666)
                     /locus_tag="AC16VR_10390"
                     /inference="COORDINATES: protein motif:HMM:NF024241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6011609.1"
                     /translation="MSRKRTGHRGSQPYSSDPQTERSLRHSIKDGVYFSAMTGGAESY
                     FSAFAIHLNASTTIIGLLASLPPLLASFMQMLSAWLGRRTGRRKPIIVAGALLQAALL
                     VPIAAVPLLWPDAAAVLLIPLVFVYLCGPNLGAPQWNSLIGDLLPEARRGRFFALRTR
                     LSSVASLAALVIAGLVLEAFDKSGDAYWGFVVIFLLAGAARCVSAYHLNAMTEPTRNV
                     AGLLAPDDLSVWRRVRHSSLLRFSLFFAAMQFAVAISGPYFTLYMLRDLALSYVEFMV
                     LSFVSVTIQFLTLNRWGRLADLFGNRLILITTGSIITIIPSLWLFSTHYGWLLVVQAV
                     SGLAWSGFTLSATAFVFDLTPSEHRATLFAVHNVLAAVAVFLGAGAGALIAAHMPSSL
                     SGFGMEVPLLTPLYGVFFVSCLMRLTVAALFLRTINEVRRVRPMSPGGLIFRVTRMHP
                     ISGLIYEIVGRSRPRSAAPTSRDPAER"
     gene            3954..4625
                     /locus_tag="AC16VR_10395"
     CDS             3954..4625
                     /locus_tag="AC16VR_10395"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016107.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR6011610.1"
                     /translation="MLVAAIAATFAGSAALAGLVFWPAGTFAWPRAWGYLALVGANFV
                     VNLVYLQRVNPSLIAARARFGAGTKRWDVIWSVCFAPAFCSIYVVAGLDAVRYEWTAM
                     SDRLWWLGLAVFVPGTSLFSWSMGVNPFFEKTVRIQTERGHRVVDAGPYQIIRHPGYV
                     GFFGWCFSAPLLLGSWWAFVPATISALGMVVRTALEDRTLQRELPGYADYARRVRYRL
                     LPGVW"
     gene            4718..5281
                     /locus_tag="AC16VR_10400"
     CDS             4718..5281
                     /locus_tag="AC16VR_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011611.1"
                     /translation="MRIEPLKGWHRYTELMNAETDPRRKAMLDNMRHHLKYECLGDPA
                     IFDSMVPDPEYRFYASFDNAVLTGMEEVREFYYNIWNTRSSLVELAIHRCAAADWGVA
                     CEGEWYQQVPGETLIANGQQVEDRDAWYLSHAHLSWFFPFREIDGRMLLEGEICYIDE
                     PGSTLQKLDPADVITLEEAKASWAALP"
     gene            5278..5799
                     /locus_tag="AC16VR_10405"
     CDS             5278..5799
                     /locus_tag="AC16VR_10405"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6011612.1"
                     /translation="MTLPISATFHHVGITVSNLERAVEWYSKVFGLKPGVALEVSGPT
                     GEQVLRLPPHIHRAALLPVGDFAIELLEFEPTRRQMDMRQDDVGYVYICFAVDDLDDV
                     YARLSAEGIDFHTPPMLAEEGQIAGSKFCVMRDPDGKTIELVEPGPGMKVPALHAAAR
                     RGVALDDPLIMDG"
     gene            complement(5827..7005)
                     /locus_tag="AC16VR_10410"
     CDS             complement(5827..7005)
                     /locus_tag="AC16VR_10410"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase family protein"
                     /protein_id="HIR6011613.1"
                     /translation="MKPQPFRIEVPEETLRDLADRLDRSGWPDDFANDDWSYGTNGAY
                     LRDLVGFWRRDYDWRAVERKINAFDHYRVEIEGMPVHFLREPGRGPNPVPLILSHGWP
                     WTFWDLSEVIRPLADPASFGGDPEDAFEVIVPSLPGFGFSTPLTTPGINFWRTADLWH
                     TLMTEVLGFQRFGAQGGDWGALVTAQLGHKYAESLFGIHLTNLIPLTLFNHQRPWDIT
                     AGVMVPDDVPADERDRQLAQQRRIASHVAVQVLDPQTLAYGLHDSPVGLLAWLLERRY
                     FWGDCNGDVESRFSREHLITTAMIYWVTSSFATSARYYAEAARNPWRPSHDRSPQVEA
                     PAGATYLGGDVGRGTIPAVREAFDLRYENSHPVGGHFAPMEEPEVVINDIRETFRPLR
                     "
     gene            complement(7012..7575)
                     /locus_tag="AC16VR_10415"
     CDS             complement(7012..7575)
                     /locus_tag="AC16VR_10415"
                     /inference="COORDINATES: protein motif:HMM:NF025050.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6011614.1"
                     /translation="MSNGTMPIFQHAYFVNDIEEAAHRWNRLYGAGPFVLVPHHKTDK
                     FQYRGTDQEADVSYGFGYLGENQIQFIQQHDETPSIYRDMYQAGEEGFHHVGCLVNDF
                     AAARQRMLDLGFVNACELWADDVNAAYFDTRSVNGGFTEIHGDPVHILGTFATWKRAH
                     QNLKPGDSPIMDMSAPFPDGRDPGYLD"
     gene            complement(7618..>8222)
                     /locus_tag="AC16VR_10420"
     CDS             complement(7618..>8222)
                     /locus_tag="AC16VR_10420"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6011615.1"
                     /translation="PGSTGLGKPLKSILHCEEDIRIFAASITPKGVETAAEVADGFFP
                     IWMDPEKFSVFEEPIRKGFAAAGDKDLSSFEVSPFVTVVIGDDVEQCMAPIRGNMALY
                     IGGMGARNKNFYNDYAKALGFEDAAVEIQDHFLAGRREEAMRAVPAELIDACHLVGPA
                     DRIRERLQRWKAAGGNGQVASMLLGTQQPEALELVASEML"
BASE COUNT         1496 a         2765 c         2660 g         1301 t
ORIGIN      
        1 gagcgtctcg agagcttgcc ccccacagcc cgccagagcg cgttgagatg gctccaggac
       61 ttcagcttcc ccgcggccga cgccgctcac atgagcgccg atgacgccgg cggcatctac
      121 tacgttgacg aagcgcccga cggcgttgag gaagccgaag gcgcggccgc cgagccgccg
      181 accagcgggg ctttgagcgc ggctgagact ttcgccctgc acagcaagcc cggcgcctcc
      241 aaggtgctct ttctcgactt cgacggccac gtcatcgaag gcacggcctg gaacgccaac
      301 agcagccggc ccgacaccta ctatgcccgc ccgttcgaca ccgacggttc gccgtcgagc
      361 ttcggctcgg cggagctgga aaccattcac gaggtctggc accgggtggc ggaggacttc
      421 gccgccttcg acgtggacgt aaccaccgag gatccgggca gcttcggccc gtccgtcggg
      481 cacgtgctcg tcaccaagga cgacgacgag ctcggcaacg ccatgcccag cgcgggcgcc
      541 ggcggggtcg cctacgtggg cgtgttcggc cgctcgaact acacctacta ccagcccgcg
      601 ctcgtctact acgacaacct gggcaacggg catcccccgt acgtggcgga agcctcctct
      661 cacgagttcg gccacaactt gagcctctcc cacgacggga cgagcaccac cagctacttc
      721 cagggactcg gaagcacctc gagcttcgtc tcgtgggcac ccatcatggg ggtgggctac
      781 taccgccagg tgacccagtg gagcaacggc gagtacgcgg acgccaacaa cacccaggat
      841 gacctgtcca tcatcaccgg ccatctcggc ccccgtggtg atgaccacgg cgacgacatc
      901 ctctcggcca gccttctcgt caccgatggc gcgggcacca tttctcacag caacccgcaa
      961 tcggatccgg gcaacttcaa tccggaaaac aaaggcatca tcgaccagca gggcgacgtc
     1021 gacgtcttcg ggctccaggt cggtaccggc acggtcgaca tcacggtgac gcccgcatgg
     1081 gacgccttca cccgcagcat gcgccggggc gcgaacctgg acatccaggc ccggctgctg
     1141 gacgacaccg gcaccgtcat cgccacgtcc gatcccggcg acgacaccga tgcgagcatt
     1201 caggttcagc tggacgccgg ctactactac ctcgccgtta ccggcgtcgg gaacagctcg
     1261 gtcccctact ccgactacgc gagccagggc atgtacttca tatcggggca ggcggtcacc
     1321 ggcgagccca acggcgcgcc caccgccaac ggcgacacgg ctggaacggc cgaggacttc
     1381 gccgtggacg tcgcggtgct ggccaacgac agcgatccgg acggcgatgc gctctcgatc
     1441 ctgtccgtca gctcgccagc aaacggcacc accgacatca gcggcaccat ggtcatctac
     1501 acgcccaacg caaacttcgc cggcaacgac gcctttacct actcggtcgg cgacggccgg
     1561 ggcggctcgg actccgcaac ggtgaccgtg accgtgaccg ggatgaacga cgcgccgagc
     1621 gcggtgaacg acgctgccgc cacggcgtcc gacaccccgg tatccgtcgc ggtgctgagc
     1681 aacgacacgg acgtcgacgg cgacgccttg agcgtggtcg gggtcggcgc ggccggcaac
     1741 ggcacggtca gccacgacgg ctccgccgtg cagtacacgc ccgcagccgg gttctccgga
     1801 acggatacgt tcggctacac cgtcagcgac ggtcaggcaa ccgcatccgc cacggtgacg
     1861 gtcaccgttt ccgccccggc accggcaacg ccgggaagct tcgccgccac ggacggcgcg
     1921 aacggcacgg cagcgctgac ctggaccgac agcgcgaacg agaccggcta cgaggtggaa
     1981 cgcgaatcgc tgcacaggaa gcgcggcgtt tgggtcggca cctccctggt ggcaacgccg
     2041 gggcaggacg ccaccagcca cacggacgcc tcgggaaccg gaaactaccg ctaccggata
     2101 cgggcggtta acggcgccgg cgcttcctcc tggaccccat ggcaggaggt cgccgttacg
     2161 gagaccagcg gcagtggtgg cggaggtggc ggcaccaagg gcggcggcaa cggccgcaag
     2221 aagtagccac cgtcagcgct cggcggggtc cctggacgtg ggcgccgccg agcgcggccg
     2281 ggatcggccg acaatctcgt agatcaaccc cgagatcgga tgcatgcggg tgacccggaa
     2341 gatgagcccc ccgggggaca tcggcctcac ccgccgaacc tcgttgatgg ttcgcagaaa
     2401 cagcgcggca accgtgaggc gcatcaggca agatacgaag aagacgccgt aaagcggcgt
     2461 tagcagaggc acctccatac cgaaaccgct cagggaagaa ggcatgtgcg cggcgatcag
     2521 ggcgccggct cctgccccga ggaacaccgc caccgcagca aggacgttgt gcaccgcgaa
     2581 cagggtcgcg cggtgttcgg atggggtcag gtcgaacacg aaagcggtgg cgctcagcgt
     2641 gaaccccgac caggcgagcc cgctgaccgc ctgaacaacc agaagccacc cgtagtgggt
     2701 agaaaacaac cacagcgacg ggatgatggt gatgatgctg ccggtggtaa tcaggatcag
     2761 ccggttgccg aaaaggtccg ccagccgccc ccaacggttc agggtgagga actggatcgt
     2821 gaccgacacg aagctcagca ccatgaactc gacgtagctc agcgccagat ccctgagcat
     2881 gtagagcgtg aagtacggcc ctgatatcgc caccgcgaac tgcatcgcgg cgaagaacag
     2941 cgagaaacgc agcagactag agtgccggac ccgccgccac accgaaaggt cgtccggcgc
     3001 cagaagcccc gccacgttgc gcgtcggctc tgtcatggca ttgagatggt aggccgagac
     3061 gcagcgcgca gcgcccgcga gcaggaagat caccacgaac ccccagtagg cgtctccgga
     3121 cttgtcgaac gcctcgagga cgagaccggc gatcaccagc gcggccaggg aggcaacgct
     3181 ggacaaccgg gtgcgcaggg caaagaaacg gccgcggcgg gcctccggaa gcagatcacc
     3241 gatgaggctg ttccattggg gtgcgccgag gtttggtcca cacaggtaga cgaacaccag
     3301 aggaatcagc agcaccgcgg cggcatcggg ccagagcagc gggaccgccg cgatgggcac
     3361 aagcagcgcc gcctgcagga gggcacccgc tacgatgatc ggcttgcggc ggccggtgcg
     3421 gcggccgagc caggcggaaa gcatctgcat gaatgatgcg agcagcggtg gcagcgaggc
     3481 gagcaacccg atgatcgtcg tgctggcatt gagatgaatg gcgaatgccg agaagtagct
     3541 ctccgccccc ccggtcatgg cggagaagta aacgccgtcc ttgatggaat ggcgcagcga
     3601 ccgctcggtc tggggatcgg acgagtaagg ttggctcccg cggtggccgg ttctttttcg
     3661 tgacaatgtc ggcttcccgc caaccggccc taaacccgga gctcccgggc gaacaatgcg
     3721 cgaaccatca cgatactctg aaactggcgc ggcgcagatt accccagcgc tgctcgcgac
     3781 accatcatcg cgcgtcggcg ggacgtcctc gcacgggccg tcgacttcca gggagatggg
     3841 tttggctatg gccggcagct gggttaacgt cgggcccgat cgatcaccgg cttgatatcg
     3901 agcgcttggc aaaccatcca gttccagcag ggtccccagg gcagatcggg aaagtgctcg
     3961 tcgccgcgat cgccgccacg ttcgccggca gcgcggcgct ggcgggcctc gtcttctggc
     4021 ctgccggcac cttcgcctgg ccccgggcgt ggggctacct tgcgctcgtg ggcgccaatt
     4081 tcgtcgtcaa tctcgtgtat ctgcagcgcg tgaatccctc cctgatcgcg gcgcgcgcgc
     4141 gtttcggcgc cggcacaaaa cgctgggacg tgatctggag cgtgtgcttc gcgccggcgt
     4201 tctgctcgat ctacgtcgtc gcgggcttgg acgccgttcg ctacgagtgg acggccatga
     4261 gcgaccggct gtggtggctg ggtctcgccg tgtttgtgcc ggggacgtca ctgttcagct
     4321 ggtcaatggg cgtcaaccct ttcttcgaga agaccgtccg gatccagacg gagcgaggcc
     4381 accgggtcgt cgacgccggc ccttaccaga tcatccggca tcccggctac gtcggcttct
     4441 tcggctggtg cttctcggcc cccctgctgc tgggttcctg gtgggcgttc gttcccgcca
     4501 ccatctcagc actcggcatg gtggtgcgaa cggccctgga ggaccggacg ctgcagcggg
     4561 agcttcccgg ctacgccgac tacgcccggc gcgtccgtta ccgcctgctc ccgggcgtct
     4621 ggtgaggacc atcgcccgac gccggcctcc ggcaggcaga gacgccgaca aatcgtgata
     4681 acttaagcag ctcgatcgtt aagaatgggg agacgagatg cgcatcgaac cgctgaaggg
     4741 ctggcaccgc tacacggagc tgatgaacgc ggagaccgac ccgcgccgca aggccatgct
     4801 cgacaacatg cggcaccacc tgaaatacga atgcctcggc gaccctgcga tcttcgattc
     4861 catggttccc gaccccgaat accgtttcta cgcgagcttc gacaacgcgg tgctcaccgg
     4921 catggaagag gtccgggagt tctactacaa catctggaac acgcgcagca gcctggtgga
     4981 gctcgccatc caccggtgcg cggccgcgga ctggggcgtg gcctgcgagg gcgagtggta
     5041 ccagcaggtg ccgggtgaaa cgctcatcgc caacggccag caggtggaag atcgtgacgc
     5101 ctggtatctg tctcacgcgc acctttcctg gttctttccg ttccgtgaga tcgacgggcg
     5161 catgctgctg gaaggcgaga tctgctacat cgacgagccg ggcagcacgc tgcagaaact
     5221 cgatcccgcc gacgtgatca cgctggaaga ggcaaaggcc tcctgggccg cgctcccatg
     5281 accctgccga tatcagcaac ctttcaccac gtcggcatca cggtgagcaa tctggagcgc
     5341 gccgttgagt ggtacagcaa ggtgttcggg ctgaagcccg gggtagcgct ggaagtttca
     5401 ggccccaccg gcgagcaggt gctgcggctt ccgccccaca tacatcgcgc cgcgctgctg
     5461 cccgtcggcg atttcgccat agagctgctg gagttcgaac cgacccgccg gcagatggac
     5521 atgcgccagg atgacgtcgg ctatgtttac atctgcttcg ccgtggacga tctcgacgat
     5581 gtctacgccc gtctcagcgc cgagggcatc gactttcaca cgccgcccat gctggccgag
     5641 gaaggtcaga tcgccggctc caagttctgc gtgatgcgcg accccgacgg caagaccatc
     5701 gagctggtgg agcccgggcc cggcatgaag gttcccgccc tgcacgcagc cgcccggcga
     5761 ggcgtcgccc tggacgaccc gctgatcatg gacggctagc cgtccccgcc atcgaccgcg
     5821 cgaaagtcac cgcaacgggc ggaacgtctc ccggatgtcg ttgatcacga cctccggctc
     5881 ctccatcggg gcgaagtggc cgcccacggg atgtgagttc tcgtagcgca ggtcgaacgc
     5941 ctcccgcacg gccggaatcg ttccacgacc cacgtcccct cccaggtagg tggcccccgc
     6001 cggcgcctcc acctggggcg agcggtcgtg gctcggccgc cacggattgc gcgcggcctc
     6061 ggcgtagtat cgggcagacg tggcgaagct ggacgttacc cagtagatca tcgccgtggt
     6121 gatcaggtgc tcgcggctga agcgggattc cacgtcgccg ttgcagtcgc cccagaagta
     6181 gcggcgttcc agcagccacg ccagcagccc cacgggggag tcgtgcagcc cgtacgccag
     6241 cgtctgcgga tcgagcacct gcaccgccac gtggctggcg atgcgccgct gctgagcgag
     6301 ctgccggtcg cgctcgtccg cgggaacgtc atcgggaacc atcacgcccg cggtgatgtc
     6361 ccacggacgc tgatgattga agagcgtcag cgggatcagg ttcgtcaggt ggatcccgaa
     6421 gaggctttcg gcgtacttgt ggcccagctg ggcagtcacc agcgcgcccc agtcgccgcc
     6481 ctgggcgccg aagcgctgaa agccgagcac ctcggtcatc agcgtgtgcc agagatcggc
     6541 cgtcctccag aagttgatcc caggggtcgt gagcggggtc gagaagccga agccgggcag
     6601 cgatggcacg atgacctcga acgcatcctc gggatctccg ccaaacgacg ccggatcggc
     6661 cagggggcgg atcacctcgc tgaggtccca gaacgtccag ggccagccgt ggctgagtat
     6721 caggggcacc gggttcgggc cgcgccccgg ctcgcgcaga aaatgcacgg gcatgccctc
     6781 gatctctacc cggtagtgat cgaaagcatt gatcttgcgc tccaccgcgc gccagtcgta
     6841 gtcccgtcgc cagaacccga ccaggtcccg caggtaggct ccgttggttc cgtaggacca
     6901 gtcatcgttc gcgaagtcgt cgggccagcc ggaacgatcg agccggtcgg ccagatcgcg
     6961 cagcgtctct tccgggacct cgatgcggaa aggttggggc ttcacggcgc ttcagtccag
     7021 atacccggga tccctgccgt ccgggaacgg tgccgacatg tccatgatgg ggctgtcgcc
     7081 gggcttcagg ttctgatgcg cccgcttcca ggtcgcaaaa gtacccagga tgtgcaccgg
     7141 atctccgtgg atctcggtga agccgccgtt caccgagcgc gtatcgaaat acgcggcgtt
     7201 aacgtcgtcc gcccagagct cgcaggcgtt cacgaaaccc agatccagca tgcgctgacg
     7261 ggcggcagcg aagtcgttga caagacagcc gacgtgatgg aagccctctt cccccgcctg
     7321 atacatgtcc cgatagatcg acggggtttc gtcgtgctgc tggatgaact ggatctggtt
     7381 ttcgcccagg tagccgaacc cgtaggatac gtccgcttcc tggtccgtgc cccgatactg
     7441 gaacttgtcc gttttgtggt ggggcacgag cacgaaaggc ccggctccgt agagccggtt
     7501 ccaccggtga gcggcttcct cgatgtcgtt gacgaagtac gcgtgctgaa agatcggcat
     7561 ggtgccgtta ctcattgctg ttgtctcctt ttcctgtgaa ggtcgtcctg agcgccccta
     7621 gagcatctcc gaggcgacca gttccagcgc ttcgggctgc tgggtgccga gcagcatgct
     7681 cgccacctga ccgttgccgc ccgccgcctt ccatctctgc agcctttccc gaatgcggtc
     7741 ggccggaccc accaggtggc aggcgtcgat cagctccgcc ggcaccgcgc gcatggcctc
     7801 ttcgcgccgc ccggccagga aatgatcctg gatctcgacg gcggcgtctt cgaacccgag
     7861 cgctttggcg tagtcgttgt agaagttctt gttgcgggct cccatcccgc cgatgtagag
     7921 cgccatgttt ccacggatgg gcgccatgca ctgctccacg tcgtccccga tgaccacggt
     7981 gacgaacggc gacacctcga aactgctcag atctttgtcg ccagctgcgg caaaaccttt
     8041 ccgtatgggc tcctcgaaca cggagaactt ttccggatcc atccagatcg ggaagaaccc
     8101 gtcggcaacc tccgcggcag tctccacacc cttcggcgtg atgctcgcgg cgaagattct
     8161 gatgtcctcc tcgcagtgca ggatgctctt cagcggtttg cccagacccg tggagccggg
     8221 gc
//
LOCUS       DBNHQR010000460         4419 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1372009, whole genome shotgun sequence.
ACCESSION   DBNHQR010000460 DBNHQR010000000
VERSION     DBNHQR010000460.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1372009"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /locus_tag="AC16VR_10425"
     CDS             complement(<1..1065)
                     /locus_tag="AC16VR_10425"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR6011616.1"
                     /translation="MTTVAGSEDTFNRSAGRQLLLFAAISIGSIGLLMYALAWASSLT
                     GSGAGSEVAVDPETRTVTLFLREEPPQLNSSLATDMVSGMVLGHVMEGLLRYDEHNRL
                     VPGMAERWEIDANGAIFHLRSDAVWSDGAPVTAHDFVFAWQLALDPANASQYAFILYP
                     VKNGRAIAEAKLPVSQLGARAVDDRTLEVTFERPVAYFDKLVAFPTYYPIRRDFYEAT
                     KGRYGADAHTLLYNGPFRMTRWVHGAHVRLEKNPTYWDEERIRIEVLDFPYITSDANA
                     TLNLFKDNKVAYAGLLAENLNDAMEQRWHIKRFMDGSVFFIEFNFRDGRVTANTNLRK
                     AMQHAVDPAELVYKVTKLPGY"
     gene            complement(1079..2578)
                     /locus_tag="AC16VR_10430"
     CDS             complement(1079..2578)
                     /locus_tag="AC16VR_10430"
                     /inference="COORDINATES: protein motif:HMM:NF017569.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR6011617.1"
                     /translation="MDRTLTNFIRALRNSEVRISTAETLDAFNTVELVGYRDRAALKR
                     SLALVLPKTADEKETFEQCFDAYFSFTDIQGSQGAAGEAERASDAETTAGGEEGGEGG
                     GAQPGEARPGDAAGSAKKKQKPGASADAEADEDEDLGAGAMSKPQSTLGQLLMANSKV
                     ELSVATAAAGEAVDVRDIEVFTQKGLYTRRIMEEMGLADLNREISELRQRPAVPDRRL
                     GQELTRRRDWLREQVRDYVEKQFLLHADASGKRLREELLRKVKLSNVEQRSFRLIQEI
                     VFRMAKKLAALHSRRKKVFKRGQINIPRTLRHNMAYDGAIFDLRWKSVKIDRPKVFAI
                     CDVSGSVANYARFMLMFLYSLEEVMPKVRSFAFSSDLAEVTELFERNAIEDAIAKTIR
                     DYSGGSTDYGQSLMDFKKLCLDEVDNRTTIIILGDARNNYGDPHTELLKELYDRCKRL
                     IWLNPEPRNAWGVGDSEMRRYAAYCHQVEECNSLMHLERIVGNLLRVVQ"
     gene            complement(2585..3535)
                     /locus_tag="AC16VR_10435"
     CDS             complement(2585..3535)
                     /locus_tag="AC16VR_10435"
                     /inference="COORDINATES: protein motif:HMM:NF012234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6011618.1"
                     /translation="MAEAAVNIEELTESVGSLRDGLESHRYICSDQIATAVYLAYHLH
                     KPILIEGPPGVGKTELAKTTAEMFALPLIRLQCYEGLDEAKAIYEWKYGKQLLYTQVL
                     KETLDEVLQGAKGFAESVTRLHEFGDIFFSEEFLEPRPLLKALKEEAGCILLIDEVDK
                     SDHEFESLLLEMLSDFQVSIPEIGTVKAVRQPPMVFLTSNNTREISDALKRRCLHLYI
                     PFPDVDLEQRIIHARVPEIPPELRRQLVQFIHELRDLDLKKVPAISETIDWARTLLLL
                     HTESLDPKLVRDTLNVILKFQEDIDNVDAEVTTLTSKALK"
     gene            complement(3660..>4419)
                     /locus_tag="AC16VR_10440"
     CDS             complement(3660..>4419)
                     /locus_tag="AC16VR_10440"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF044908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR6011619.1"
                     /translation="GQVLGPALVAASRTVPEDRHAHSLHGYFLRGGDTTVPILYTVDR
                     IRDGRSFTTRRVVAIQHGKAIFNMAISFQIDEPGLSHQMPMPEVPDPEGLPDESELRK
                     AWADKLPEPLEAAFDRERPIEVRPIDPVDVFAPEKRPPQQMCWMKCRDRLPDDPGLHQ
                     CVLAYLSDWSLLDTATLPHGVSFMQDNMQMASLDHAMWFHRPFRADEWLLYVQDSPSA
                     SGARGLNRGLIYNRAGELVASAAQEGLIRLHETD"
BASE COUNT          713 a         1407 c         1450 g          849 t
ORIGIN      
        1 gtagccgggc agcttggtca ccttgtagac cagctccgcc gggtccacgg cgtgctgcat
       61 ggccttgcgc aggttcgtgt tcgcggtgac gcgcccgtcg cgaaagttga actcgatgaa
      121 gaagaccgag ccgtccatga atcgcttgat gtgccagcgc tgctccatgg cgtcgttgag
      181 attctccgcc agcagcccgg cgtaggcgac cttgttgtcc ttgaagagat tcagcgtcgc
      241 gttggcgtcg gaagtgatgt acggaaagtc gagcacctcg atgcggatcc gttcctcatc
      301 ccagtacgtg gggttcttct ccagcctcac gtgggcgccg tgcacccagc gcgtcatgcg
      361 gaacgggccg ttgtagagca gcgtgtgcgc atccgcgccg tagcgaccct tggttgcctc
      421 gtaaaaatcc ctgcggatcg ggtagtaagt cgggaaagcc acgagcttgt cgaagtaggc
      481 caccggccgc tcgaacgtga cctcgagggt gcggtcgtcc acggcccgcg cgccgagctg
      541 cgaaacgggg agcttggcct cggcgatggc ccgcccgttc ttcaccgggt agaggatgaa
      601 ggcgtactgg gacgcgttgg cggggtccag ggccagctgc caggcgaaca cgaagtcgtg
      661 ggcggtgacg ggcgcaccgt cgctccacac cgcgtcgctg cgcaggtgaa agatggcgcc
      721 gttggcgtcg atctcccagc gctcggccat acccggcacc agccggttgt gctcgtcgta
      781 gcgcagcagg ccttccatca cgtggcccag caccatgccg ctgaccatgt cggtggccag
      841 ggagctgttc agctgggggg gttcctcgcg caggaagagc gtgaccgtgc gtgtctccgg
      901 atccacggca acctcggagc cagcgccgga cccggtcagg ctggaagccc aggccagcgc
      961 gtacatcagc agcccgatgc tgccgatgct gatggcggcg aacaggagaa gctgccgccc
     1021 ggcggaccgg ttgaaggtgt cctcgcttcc tgcgaccgtt gtcaccgcgc tccgcttcct
     1081 actgaacgac ccgcagcaga ttacccacaa tgcgctcgag atgcatgagg ctgttgcact
     1141 cttctacctg atgacagtac gccgcgtacc ggcgcatctc cgagtcccct acgccccagg
     1201 cgttacgcgg ctcgggattc agccagatca gccgcttgca gcgatcgtag agctccttta
     1261 agagctccgt gtgcggatcg ccgtaattgt tgcgggcatc gccgaggatg atgatggtcg
     1321 tgcggttgtc cacctcgtcc aggcaaagct tcttgaagtc catgagcgac tggccgtagt
     1381 ccgtcgagcc gccactgtag tcgcggatgg tcttggcgat ggcgtcctcg atggcgttgc
     1441 gctcgaagag ctcggtcacc tcggccagat ccgacgagaa ggcgaaggag cgcaccttgg
     1501 gcatgacctc ctccaggctg tacaggaaca tcagcatgaa gcgcgcgtag ttggcgacag
     1561 agccggagac gtcgcagatc gcgaaaacct tgggccggtc gatcttcaca gatttccagc
     1621 gcaggtcgaa gatggctccg tcgtaggcca tgttgtggcg cagggtgcgc ggaatgttga
     1681 tctgccccct cttgaacact ttcttgcggc gcgaatgcag agcggccagc ttcttggcca
     1741 tccggaagac gatttcctgg atgaggcgga agctgcgctg ctccacgttg gacagcttta
     1801 ccttgcgcag cagctcttcg cgaagccgct tgcctgacgc gtcggcgtga aggagaaact
     1861 gtttctccac gtagtcgcgc acttgctcgc gcagccagtc ccggcgccgg gtgagctcct
     1921 ggccgaggcg gcgatcaggc acggccgggc gctggcgcag ctcggaaatc tcccggttca
     1981 gatccgcaag gcccatctcc tccatgatcc ggcgggtgta gagccctttc tgagtgaaca
     2041 cctcgatgtc gcggacgtcg acggcctctc cggccgccgc cgttgccacg gacagctcga
     2101 ccttggaatt ggccatgagc agctgcccga gggtcgactg cggcttgctc atcgcgccgg
     2161 cgcccagatc ctcgtcctcg tctgcctccg cgtcggcgct tgcgcccggc ttctgttttt
     2221 tcttcgcgct gccagcggca tcacccgggc gcgcttcgcc gggctgagcg ccgccgcctt
     2281 caccgccttc ctcgccgcct gcggtggtct cggcgtcgga ggcgcgctca gcctcgccgg
     2341 ccgctccctg gctgccttgg atgtcggtga aggagaaata cgcatcgaag cactgctcga
     2401 acgtctcctt ctcgtccgcc gtcttgggta gcaccagcgc aagcgagcgc ttgagcgcgg
     2461 cccgatcccg atagcccacg agctccacgg tgttgaaggc gtcgagcgtc tccgccgtgg
     2521 agatgcgcac ctcggaattg cgcagcgcgc ggatgaagtt ggtcagggtc cggtccatcc
     2581 ggacttattt gagcgccttg ctggtcagcg tggtcacttc cgcgtccacg ttgtcgatgt
     2641 cctcctggaa cttgaggatc acgttgaggg tgtcccggac cagcttgggg tccagcgact
     2701 cggtgtgcag gagcagcagc gtgcgcgccc agtcgatggt ctcgctgatg gccggcacct
     2761 tcttcaggtc cagatcgcgc agctcgtgaa tgaactgcac cagctggcgt ctcagctccg
     2821 gcgggatctc cgggacccgg gcgtggatga tgcgctgctc gagatcgacg tctggaaacg
     2881 gaatgtacag atgcaggcag cggcgcttga gcgcgtcgga gatctcccgg gtgttgttgc
     2941 tggtgagaaa caccatgggc ggctgccgca cggccttcac cgtgccgatc tcgggaatgg
     3001 acacctggaa atcggacagc atctccagca ggagcgattc gaactcgtga tcggacttgt
     3061 ccacctcgtc gatgagcagg atgcagccgg cttcctcctt cagggccttg agcagcgggc
     3121 gcggctctaa aaactcctcg gagaagaaga tgtcgccgaa ctcgtgcagc cgcgtcacgg
     3181 attccgcaaa gcccttcgct ccctgcagaa cctcgtcgag cgtctccttg agcacctggg
     3241 tgtacaggag ctgcttgccg tacttccact cgtagatggc cttggcctcg tcgagcccct
     3301 cgtagcactg cagacggatc agcggcagcg caaacatctc tgccgtcgtc ttggccagct
     3361 ccgtcttgcc tacccccgga gggccttcga tgaggatcgg tttgtgcagg tggtaggcga
     3421 ggtagacggc cgtggcgatc tggtcgctgc agatgtagcg atgtgactcg aggccgtccc
     3481 tgaggctgcc cacggactcg gtgagctctt cgatgttcac tgccgcttcg gccactgctt
     3541 cccccagccc ggctcttacc tgggcgtgcg tatttcgcgc tgcacaaaac ccgctattat
     3601 cgctgctccc cttcagcctt gtacaccgcg ctggcgagcg atccgttggc catcgccgat
     3661 cagtcggtct cgtgaagccg gatcaggcct tcctgggcgg ctgacgccac cagctctcct
     3721 gcgcggttgt agatcaggcc acggttgagg ccccgggcgc cgctggcgct ggggctgtcc
     3781 tgaacgtaga gcagccactc gtcggcacgg aaaggacggt gaaaccacat ggcgtggtcc
     3841 aggctcgcca tctgcatgtt gtcctgcatg aagctcaccc cgtgggggag ggtggcggtg
     3901 tccagcaggg accagtcgga aaggtaggcc agcacgcact ggtgcaggcc cgggtcatcg
     3961 ggcaggcggt cgcggcactt catccagcac atctgctgtg gcggccgctt ctccggcgcg
     4021 aagacgtcca ccgggtcgat gggacgcacc tcgatgggcc tttcccggtc gaaggccgcc
     4081 tccagcggct cggggagctt gtcggcccat gccttacgca gctcgctctc gtcgggcagc
     4141 ccctctggat ctggcacttc gggcatgggc atctgatgag acagcccggg ctcgtcgatc
     4201 tgaaaggaga tggccatgtt gaagatggcc ttaccgtgct gaatggctac cacacgacgg
     4261 gtggtgaagc tgcgcccgtc ccggatccgg tcgacggtgt acaggatggg caccgtggtg
     4321 tcgccgccgc gcaggaaata gccgtgcagg gagtgggcgt gacggtcctc gggtacggtg
     4381 cgtgaggcgg ccaccagggc cggccccaga acctgcccc
//
LOCUS       DBNHQR010000461         2919 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_297639, whole genome shotgun sequence.
ACCESSION   DBNHQR010000461 DBNHQR010000000
VERSION     DBNHQR010000461.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_297639"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="AC16VR_10445"
     CDS             <1..620
                     /locus_tag="AC16VR_10445"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6011620.1"
                     /translation="TYLQAFLARFSGEVRLADPVQAVARTASGVDITSASGTARFDAV
                     VLACHSDEALGLLADPSEAERDVLGAIGYQPNRVVVHSDDRVMPEHRPAWSSWNALVG
                     AERPERCQVSYWMNLLQSLPGRENFFVTLNPQMPLKRVWSVREYAHPVFTRRARAAQR
                     RKAEISGVRNTWFCGAYWGWGFHEDGFVSGMEVAEGMLGSMADAA"
     gene            610..1368
                     /locus_tag="AC16VR_10450"
     CDS             610..1368
                     /locus_tag="AC16VR_10450"
                     /inference="COORDINATES: protein motif:HMM:NF018770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HIR6011621.1"
                     /translation="MPPELLRDSVCEGWVYHRRAAPEHRFRYRVWMLWLDVARLGQGP
                     LRLLRRRLGLSPLGIAPKDFLDGSDDLLGAVRARLASHGYPAPARVFLLTQPRSWISG
                     FNPVSFYFCVGQAGLDCILAEINNTPWDERHTYVLDARGQSGEFSFSFPKAFHVSPFM
                     PMDLEYRWRFAIEPEAVKIAMGLFREGEETFNAGLRLSVVPMTRRRLLLGMARYPLQN
                     VATLARIYWQALRLYSKRSRFYPHPDRAEEAPAS"
     gene            1365..2618
                     /locus_tag="AC16VR_10455"
     CDS             1365..2618
                     /locus_tag="AC16VR_10455"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6011622.1"
                     /translation="MTDNTLYGDGRRTAPSGPFAALARKALGRLRDGTIRLTEAGRTE
                     VFGCGAPEVHVEVRDPRFFRRLVMGGSVGAGESYMDADWSCDDLVSLIRIFARNRDAL
                     DVVDGAGSVLVRWANRSRHLLRRNTRRGSRRNIEAHYDLSNEFFETFLDGRMMYSSAV
                     FESPDATLEEASEAKLERLCRKLELGPEDHLLEIGSGWGGLAVYAAAQFGCRVTTVTI
                     SPAQLEATQARVRAAGLQGRVRAVLSDYRDLDGCYDKVVSVEMVEAVGHEFLDRYFES
                     LSGLTRPGGLIALQAITIADRRYRAALAEADFIKKHIFPGSFIPSVCVLVDAAARRTD
                     AVLVNLEDIGLDYARTLRAWRERLEARRGEIQSLGFDERFLRMWRFYLAYCEGGFLER
                     AISDVQLLFAKPAYRGQPWRGALDG"
     gene            2652..>2919
                     /locus_tag="AC16VR_10460"
     CDS             2652..>2919
                     /locus_tag="AC16VR_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011623.1"
                     /translation="MSILIDLAEARRFPDALVRAGIRRLVAQRLKDEGSQGVAAQAER
                     HQRLLRELQESPVALDTELANEQHYEVPAEFFRAVLGKHLKYSSC"
BASE COUNT          450 a          940 c          991 g          538 t
ORIGIN      
        1 ccacctatct gcaggcgttt ctggcccgtt tcagcggcga ggtgcggctg gccgacccgg
       61 tgcaggcggt agccaggacg gcgtcgggcg tggacatcac ctcggcgtcg ggaaccgcgc
      121 gcttcgatgc cgtcgtgctg gcctgccaca gcgacgaggc cctggggctg ctggccgacc
      181 ccagcgaggc ggagcgggac gtgctgggcg ccatcggcta ccagccgaac cgggtcgtcg
      241 ttcactccga cgaccgggtc atgcccgagc atcggccggc gtggtcgagc tggaacgcct
      301 tggtcggggc cgagcgcccc gagcgttgcc aggtgagcta ctggatgaac ctgctccagt
      361 cattgcccgg gcgcgaaaac ttttttgtga cgttgaaccc gcagatgccc ctcaagcgcg
      421 tgtggagcgt gcgcgagtac gctcacccgg ttttcactcg ccgtgcccgt gcagcccagc
      481 gtcgcaaggc cgagatcagc ggcgttcgca atacctggtt ctgcggtgct tactggggct
      541 ggggctttca cgaggacggc ttcgtcagcg gcatggaggt tgccgaaggc atgcttgggt
      601 ctatggccga tgccgcctga gctcctccgc gacagcgtct gtgaggggtg ggtttaccac
      661 cgccgcgccg ccccggaaca ccgtttccgg taccgcgtct ggatgctctg gctcgatgtt
      721 gcccgcctcg gccaggggcc cttgcgcctg ctgcggcgcc ggctgggact gagcccgctc
      781 gggatagcgc ccaaggactt tctcgacggc agcgacgacc ttctcggcgc ggttcgcgcg
      841 cgcctggcca gtcacggcta cccggcgccg gcccgggtgt ttctgctcac ccagccccgc
      901 agctggattt ccgggttcaa tccggtcagc ttctacttct gcgtgggcca agcagggctt
      961 gattgcattc ttgccgagat caacaacacg ccctgggatg aacgccatac ctacgtgctg
     1021 gacgcgcgcg gccagtcggg cgagttctcc ttctcttttc ccaaggcctt tcacgtctcg
     1081 cctttcatgc cgatggatct cgaataccgt tggcggttcg ccatcgagcc cgaagcggtg
     1141 aagatcgcca tgggtctgtt cagggaaggg gaagagacct tcaacgccgg gctgaggctg
     1201 tcggtagtgc ccatgacccg gcgccgcctg ctcctgggca tggcccgcta tccgctgcag
     1261 aacgtggcga cgctggcacg aatctactgg caggccctcc ggctctattc caagcgcagc
     1321 cgtttctatc cgcaccccga tcgcgctgag gaggcgccgg catcatgacc gacaacacct
     1381 tgtatggcga cggccgacga accgcgccct cgggcccgtt tgccgcgctg gctcgcaagg
     1441 cgctgggcag gttgcgagat ggcaccatcc ggctgaccga ggcgggccgt accgaggttt
     1501 tcggttgcgg cgccccggag gtgcacgtgg aggtccgtga tccgcgtttc ttccggcgcc
     1561 tggtcatggg cggaagcgtc ggggccggcg agtcatatat ggacgcggac tggtcctgcg
     1621 acgatctggt gtctctgatc agaatctttg cccgcaaccg cgacgccctg gatgtggtgg
     1681 atggcgccgg cagcgttctt gtgcgatggg ccaaccgctc caggcacctg ctgaggcgca
     1741 acacccgacg gggaagccgg cgcaacatcg aagcgcacta tgacctgagc aacgagttct
     1801 tcgagacttt tctcgacggc aggatgatgt actcgtcggc cgtgttcgag tcgccggatg
     1861 caaccctgga ggaggcgagc gaagccaagc tggagcggct gtgccgaaaa ctcgagctcg
     1921 gcccggagga tcaccttctg gagatcggct cgggctgggg cgggctcgcc gtgtacgcgg
     1981 cagcgcagtt cggctgtcgg gtcaccacgg tcacgatttc ccccgctcag ctcgaagcca
     2041 cccaagcgcg ggttcgggcc gccgggctcc agggccgcgt cagggccgtt ctgtcggact
     2101 atcgcgatct ggacggctgt tacgacaagg tggtatccgt tgagatggta gaggcggttg
     2161 ggcacgagtt cctcgatcgc tacttcgagt cgctgtccgg actcacccga ccaggtgggc
     2221 tcatcgccct gcaggcaatc accattgcag accggcggta ccgcgcagcg ctcgcagaag
     2281 cagacttcat caagaaacac atcttccccg gcagcttcat tccctcggtc tgcgtgctgg
     2341 tggacgcggc ggcgcgccgg accgacgcgg tgctggtcaa cctcgaggac atcggcctcg
     2401 actacgcgcg caccctgcgc gcctggcgtg agcgcctgga agcccggcgc ggggagattc
     2461 agtcgctggg cttcgacgag cggtttctgc gcatgtggcg gttttacctc gcttattgcg
     2521 aaggcgggtt cctggagcgg gccatcagcg acgtacagct tcttttcgca aagcccgcgt
     2581 accgggggca gccctggcgg ggtgccctcg acggttgagg gggcggctcg aatcaacaag
     2641 gagacggttt catgagcata ctcatcgatc tggccgaggc gcgccgattt ccggacgccc
     2701 tggttcgcgc gggtatccgc aggctcgtcg cgcagcggct gaaagacgag gggtcgcaag
     2761 gcgtcgccgc tcaggcggag cgacaccagc gcctgctgcg cgagctccag gaaagccccg
     2821 ttgcgctgga tacggagctg gccaacgagc agcactacga ggtccccgcc gagttctttc
     2881 gtgcggtgct tggaaagcac ctcaagtaca gcagctgtt
//
LOCUS       DBNHQR010000462         5256 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_44450, whole genome shotgun sequence.
ACCESSION   DBNHQR010000462 DBNHQR010000000
VERSION     DBNHQR010000462.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5256)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_44450"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="AC16VR_10465"
     CDS             <1..768
                     /locus_tag="AC16VR_10465"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013692821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6011624.1"
                     /translation="VVVGGGQTPGETIGNGRATAIQFAREGARVLVGDRDVGSAEETV
                     AMILDEGGEAASAEIDVTVEESVASFMAAAVDRWGRIDVLHNNVGVGLAGADAGVEEI
                     SADVFDQLVRINLRGMVLTAKHVLPGMRERGSGVIINISSMAAWKPYPFVGYKTTKAG
                     VIALTEQLAQANARYGIRANVILPGLMNTPMAVEPRVSQTMTREEVIADRDRRVPLGR
                     KMGTAWDVAHAAAFLASDRARFITGVALPVDGGASVS"
     gene            complement(808..1647)
                     /locus_tag="AC16VR_10470"
     CDS             complement(808..1647)
                     /locus_tag="AC16VR_10470"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6011625.1"
                     /translation="MTTGRPVAVVIGATSKWQADGRNTRLAHGHDVDDSDLPVSARWG
                     IGGAVAQKFAREGFLVVLTTRTAANAAGLEAAIAEQGGECMTVELDLVSERSVSEAFA
                     TIRREAGEPEVVVYNAGYLEGRDLPPDQELLEHIPVEMFDTAQHISSRGPFLVAKEIL
                     PGMRERGSGSFLITNNAASLRGRKRYTGQSLYYPRVMMRTLAQVLTEEYSEHGVHVAN
                     IIVDGLIDSPGTRALPMAQQRPEIIMNPAAIADAYYYLHTQDRSCWTHELQLTPHSTK
                     PSF"
     gene            1824..2495
                     /locus_tag="AC16VR_10475"
     CDS             1824..2495
                     /locus_tag="AC16VR_10475"
                     /inference="COORDINATES: protein motif:HMM:NF018961.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HIR6011626.1"
                     /translation="MNTFEAIEDLLARCERLQDVAVDSLDEVCSSLGVQLARRFLSER
                     KHLYRRYLAFCLEDKELTEAEHADLAHLQHLLYLGAADVALIHDDVAHEVYGKALDEV
                     LSDLKISAEEEAFLRRLRSELKLSEAEAAVMLEAGRERMRDVALEQASTSDPEFSKPR
                     APIGQFVGRSDESLEQAVRDAVSKARLAIPRLHWFEVGNISGYVDDEGPKSWHVTVRG
                     GIKTS"
     gene            2587..3711
                     /locus_tag="AC16VR_10480"
     CDS             2587..3711
                     /locus_tag="AC16VR_10480"
                     /inference="COORDINATES: protein motif:HMM:NF019640.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6011627.1"
                     /translation="MASLAWARRGETEQDRRLAPPLVEALAASRLCRLLLPGSLEGAG
                     VTPPAFLDVLAILSASEASLSWMVWNNALPCLLARYLQRSARAEIFADPGWLYASSTR
                     PSGRAVREEHGYRVSGRWALVSGCEHAEWIAFLLIVEEGGETSMLTPEVPEMRMAFLR
                     RGAYEIEDTWHTGGLRGTGSHHVRVTGQLVPERFTFSLADPPGDAHPLERLPVFCNMS
                     AGFAAQMLGIAGMARETLVTLARTKTTGSRMPDLRDRSAAQSFAAAQSAAGEAARSYL
                     YASTEQVWEDAVAGRTGDPAHVAALFAAAQHAIAIARDTVDGAFALVGTAALYVDNPL
                     ERAHRDMHAMLRHILAQPSWLEQAGRLEFGLEITEPMFFV"
     gene            3783..4514
                     /locus_tag="AC16VR_10485"
     CDS             3783..4514
                     /locus_tag="AC16VR_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003210139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6011628.1"
                     /translation="MTTSEKVVLVIGAGVATGGAVARRFAREGFTACMARRQGEKLQG
                     LVDEIAAAGGRAHGYSCDATEEAQVVDLVERVEAEVGPIEVACYNAAVGASHSITEFP
                     ADAYERVWRINTFGAFVMGREVSRRMVERGRGTILFTGATSALRGKARLAAFAGSKHA
                     LRALSESMARELFPQNIHVAHVIIDGPIDTPLIRRVMPSVFEERPADGVLLPDDIAET
                     YWNIHCQPRSAWLHETQLRPWLEPW"
     gene            4536..5126
                     /locus_tag="AC16VR_10490"
     CDS             4536..5126
                     /locus_tag="AC16VR_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR6011629.1"
                     /translation="MSKTVEIFFDFLSPPSYLAWTQVPGIVQRTGASAVWRPMFTIGL
                     HQLTENRSPVMVPNKARWIAGDLQRFARRYGVTLNPNPHGIINILPADRAAALAEKEG
                     AVEQLMATAYPAMWVDGRDLSDPEVLGEVIAAAGLDASRYLAAIETDEVKNLLKQNTQ
                     EAADRGAFGAPTFFVGDEMFFGQDRLDFVEEALAGG"
     gene            5128..>5256
                     /locus_tag="AC16VR_10495"
     CDS             5128..>5256
                     /locus_tag="AC16VR_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004350408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6011630.1"
                     /translation="MAQAYDYIIVGAGSAGCVLAYRLSADPGVNVLLLEAGGVDRSP"
BASE COUNT          828 a         1722 c         1803 g          903 t
ORIGIN      
        1 gtggttgtgg gcggtggcca gacgccgggg gagaccatcg gcaacggccg cgccacggca
       61 atccagttcg ctcgggaggg cgcgcgggtc ctggtgggcg accgcgacgt gggctcggcg
      121 gaggaaaccg ttgccatgat cctcgatgaa ggcggcgagg ccgcgtccgc cgaaattgac
      181 gtgacggtgg aagagagcgt cgcctcgttc atggctgccg ccgtcgaccg ttggggacgc
      241 atcgacgtgc tccacaacaa cgtcggcgtc gggcttgccg gggccgatgc cggcgtggaa
      301 gagatctcgg cggacgtgtt cgatcagctg gtgcgcatca acctgcgcgg catggtgctc
      361 accgccaagc acgttctgcc cggcatgcgc gagcggggct caggagtgat catcaacatc
      421 tcttccatgg ccgcgtggaa gccgtatccc tttgtgggtt acaagaccac gaaggccggt
      481 gtcatcgccc taaccgagca gctcgcccag gccaacgccc gctatggcat tcgagccaac
      541 gtgatcctgc ctggcctgat gaacacgccc atggccgtgg agccccgcgt ctcccagacc
      601 atgacccggg aggaggtcat cgccgaccgt gaccggcgtg tgccgctggg gcgcaagatg
      661 ggaacggcct gggacgtggc ccacgcggca gcctttcttg cctccgaccg cgcccggttc
      721 attacggggg tcgctctccc cgtggacggc ggcgccagcg tcagctgagg ctttggctgg
      781 gcgggccgct tgtcctgcag gcgaggctca gaagcttggc ttggtcgagt gcggcgtcag
      841 ctgcagctcg tgggtccagc atgatctgtc ctgggtgtgc aggtagtagt aggcgtcggc
      901 gatggcggcc gggttcatga tgatctccgg gcgctgctgc gccatgggca gagcccgtgt
      961 gcccggcgag tcgatgagcc cgtcgacgat gatgttggcc acgtggacgc catgttccga
     1021 gtactcctcg gtcaacacct gggccagcgt gcgcatcatc accctcgggt agtacagcga
     1081 ctgccccgtg tagcgcttac gaccccgcag tgaggccgcg ttgttggtga tgaggaacga
     1141 tccggagccg cgttcgcgca tgcccggcag gatctccttg gcgacgagaa aggggccgcg
     1201 gctggagatg tgctgggcgg tgtcgaacat ctccacggga atgtgttcca ggagttcctg
     1261 atcgggcggc aggtcccggc cctcgaggta gcccgcgttg tagaccacca cctcgggctc
     1321 gccggcctcc cgtcggatgg tcgcgaaggc ttcgctgacg gaccgctccg acaccaggtc
     1381 gagctcgacg gtcatgcact cgccgccctg ctcggcgatg gccgcctcca ggccggccgc
     1441 gttggccgcc gttcgtgtgg tcagcaccac gagaaagcct tcacgcgcga acttctgcgc
     1501 caccgcgccg ccgatgcccc aacgcgccga gacgggcagg tcggaatcgt ccacgtcgtg
     1561 cccgtgggcg agacgggtgt tgcggccgtc ggcctgccac ttggaagtgg cgccgatgac
     1621 cacggcaacc ggtctgccag tcgtcatgtc tacgatctcc cgtgttcgtg ctgtttccga
     1681 aactgtatcc catgaagcgc cgccggtacc atgtcccccc cgtgggaatc acctcaaacg
     1741 attggaaaca cgacgtggca gcaccctttc gcccggctca gccggccagc cccggcctct
     1801 cgcgcagcat ccgatccaag cgcgtgaata cctttgaggc catcgaggat cttctggccc
     1861 gttgcgaacg gctgcaggac gtcgccgtcg acagcctgga cgaagtctgc agcagcctgg
     1921 gcgttcagct tgcccgccgt ttcctgagcg agcgcaagca tctctaccgg cgctatctgg
     1981 cgttctgcct cgaggacaag gagctcaccg aagccgagca cgccgatctc gcccatctgc
     2041 agcacctgct ttatctgggc gcggcggacg tagcccttat tcacgacgat gttgcccacg
     2101 aagtttacgg gaaggcgctg gacgaggtgc tgtcagatct gaagatcagc gccgaagagg
     2161 aagcttttct gcgccggttg agaagcgagc tgaagctctc tgaggcggaa gccgccgtga
     2221 tgctcgaagc cggccgggag cggatgcgcg atgtggcact cgagcaggcg tcgacgtcgg
     2281 acccggagtt ttcgaagccc cgagcgccga tcggtcagtt cgtcgggcgc tcggacgagt
     2341 ccctggagca ggcggtgcgc gacgccgtgt ccaaggcccg gctcgccatc ccccggctgc
     2401 actggttcga ggtcggcaac atctccgggt atgtggacga cgaaggcccg aagagctggc
     2461 acgtcaccgt gcgcggcggc atcaagacca gctgacgggc atggcgcggc gcccccacct
     2521 ggagcgcatc gtcaacgagt tgaggcgtaa ccgatgaggg acgggctgac aagcaaagca
     2581 acggaattgg catccctggc atgggcacgt cgcggcgaga ccgagcagga tcgccgcctc
     2641 gccccgccgc tggtcgaagc gctggccgcc agtcggctct gtcgcctgct gctgcccgga
     2701 tccctcgagg gcgccggcgt cacgcccccc gcctttctcg acgttctggc gatcctgtcc
     2761 gcctcggagg cctcgctctc ctggatggtc tggaacaacg ctctgccctg cctgctcgcc
     2821 cgctatctac agcggtccgc ccgggcggag atattcgcgg atccgggttg gctctacgcc
     2881 agctcgacgc ggccatcggg gcgggccgtg agggaggagc acggctaccg ggtgagcggc
     2941 cgctgggctc tggtctcggg ctgcgagcac gccgagtgga tcgccttcct gctcatcgtc
     3001 gaggagggcg gcgagacgag catgctcacc ccggaggtgc ccgagatgcg catggctttc
     3061 ctgcgcaggg gcgcctacga gatcgaggac acctggcata ccggaggctt gcgcggcacg
     3121 ggcagccatc acgtgagggt gaccggtcag ctggtccccg aaagattcac gttttccctg
     3181 gccgacccgc ccggggacgc ccatcctttg gagcggttgc ccgtattctg caacatgtcg
     3241 gccggtttcg ccgcccagat gctgggcata gcggggatgg ctcgggagac gctcgtgacg
     3301 ctcgcgcgca ccaagaccac cgggtcgcgc atgcccgact tgcgtgaccg gtccgccgcc
     3361 cagtcgtttg cggccgcgca gagcgcggcc ggcgaggcgg cccgaagcta cctttacgca
     3421 tctacggagc aggtctggga agatgcggtg gctggccgga cgggcgaccc tgcgcacgtc
     3481 gccgcgctct tcgccgccgc ccagcacgcc atcgcgatcg cccgggatac tgtggacggt
     3541 gcctttgccc tcgtcggcac cgccgccctc tacgtggaca accccctcga gcgcgcccat
     3601 cgcgacatgc acgcgatgct ccgccacatc ctcgcccagc cgtcctggct ggagcaggcg
     3661 ggccggctgg agttcgggct cgagatcacg gaaccgatgt tcttcgtctg aactcctgac
     3721 cggcggctcg cgctatattc gggaatccgg gtgggttact gtcgggccaa gggggattgc
     3781 ctatgacaac ttcggagaag gtggtgctcg tcatcggggc cggggtcgcc accggcggcg
     3841 cggtggcgcg gcgttttgcc cgggagggtt tcaccgcctg catggcgcga cgtcagggag
     3901 aaaagctgca ggggctggtg gacgagatcg ccgcggcggg cggacgggcc catggttaca
     3961 gctgcgatgc gaccgaagaa gcccaggtcg tggatctggt cgagcgggtg gaagccgagg
     4021 tcggacccat cgaggtggcc tgctacaacg ccgccgtggg tgcatcccac agcatcaccg
     4081 agttcccggc agatgcctac gagcgcgtct ggcgcatcaa caccttcggt gccttcgtca
     4141 tggggcggga ggtgtcccgg cgcatggtgg agcgggggcg tgggaccatc ctcttcacag
     4201 gcgccacctc cgcgctgcgc gggaaagccc ggctcgcggc ctttgcaggg tcgaagcacg
     4261 cgcttcgcgc attgagcgag agcatggccc gggagctttt cccgcagaac atccacgtgg
     4321 cccacgtgat catcgacggg cccatcgaca cgccgctcat ccgccgcgtg atgccgtcgg
     4381 ttttcgagga gcgtcctgcg gacggcgttc tgcttcccga cgacatcgcc gagacgtatt
     4441 ggaacattca ctgccagccc cgcagcgcat ggcttcacga aacccagctg cgcccctggc
     4501 tggagccctg gtagacggtt ggaggtggtg tcatcgtgag caaaaccgtg gaaattttct
     4561 tcgacttctt aagcccgccg agctatctgg cgtggaccca ggtgccgggc atcgtccagc
     4621 ggaccggcgc cagcgccgtc tggcgaccca tgtttaccat cggcctgcac cagcttacgg
     4681 agaaccgttc gccggtcatg gtgccgaaca aggcacgctg gatagccggc gacctgcagc
     4741 gatttgcgcg ccgttacggc gtcaccttga atcccaatcc ccatggcatc atcaacatcc
     4801 tgccggccga ccgtgccgct gcgctggcgg agaaagaggg cgcagtggag cagctcatgg
     4861 ccacggcgta cccggccatg tgggtggacg gccgcgacct gtcggacccc gaggtgctcg
     4921 gcgaggtcat cgctgccgcc ggtctcgatg catcccgcta ccttgccgcc atcgagaccg
     4981 acgaggtgaa gaacctgctc aagcagaaca cccaggaagc tgccgatcgg ggtgccttcg
     5041 gcgctccgac cttcttcgtg ggcgacgaga tgttcttcgg ccaggatcgc ctggatttcg
     5101 tcgaagaagc gctggcgggg ggctgagatg gcccaggcgt acgactacat catcgtcggt
     5161 gctggttcgg ccggctgcgt gctggcctat cggctctccg cagatcccgg cgtgaacgtt
     5221 ctgctcctgg aggcgggtgg ggtggacagg agcccc
//
LOCUS       DBNHQR010000463         3150 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_106948, whole genome shotgun sequence.
ACCESSION   DBNHQR010000463 DBNHQR010000000
VERSION     DBNHQR010000463.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_106948"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="AC16VR_10500"
     CDS             complement(<1..517)
                     /locus_tag="AC16VR_10500"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR6011631.1"
                     /translation="MQEFHVNVATPDGEMECFAAHPDGEGPFAAVILYMDAPGIREEL
                     RDFCRRIAGQGYFCLLPDLYYREGRVRFDLRKGEEELKRMFAVGSRLTVEAVMRDTGG
                     MLAYLEDNPLARSPTGCIGYCMSGQFVVAAAGTFPGEIAAAASLYGTRIVTDKPDSPH
                     KLAERMKGELYL"
     gene            691..2421
                     /locus_tag="AC16VR_10505"
     CDS             691..2421
                     /locus_tag="AC16VR_10505"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl-D-amino-acid deacylase family protein"
                     /protein_id="HIR6011632.1"
                     /translation="MHLDLVIRNGMLIDGRRTPSYRADVGIRDGKVAVIGRIAPGSGD
                     RELDADGLIVAPGVVDLHTHYDSQIFWDPWCRISGQHGVTSVVVGNCGFGFAPVRPEH
                     RDRAMLTMARNEAVPLECMQQGMPWDWTTYPEFLDSLDRTPKGVNVLSYVGLNPLMVW
                     LTGSVEEAKGRPLTRAEKAEAERLLAEAMDAGACGWSAQFIGADTVQRDHDGTPMITD
                     LMSREDIEFFAAALGRLGRGGIQMIGPPKMADVLAAASGRPVIYNVLAARSDQHGSPA
                     ESYKDVIAWLARANAEGNRIIAHAVTCAIDYQFTMEDWNLFDSSPLWRELTIGTVPER
                     KAKMMDAQRRAALHEEWAAGKAPIAGGGTEQREERIGVKFQDLVLAYVTEEGEHLRSW
                     EGHTVAEIAAGMGVHPIDAFLDVSIACDLKAGWQSEPRTIDPQVMRDIANCPYAVPGL
                     SDGGAHTKFLTTGTYPTEFLATWVRDNGVMDLEEAHWRLSAYSAQTAGLTDRGFIAEG
                     MPADIVVYDLQALELLPSERVADWPGGAWHLDRRANGWRWTIVNGAVTFADGVCTGAT
                     PGRLLRHGHA"
     gene            2418..3053
                     /locus_tag="AC16VR_10510"
     CDS             2418..3053
                     /locus_tag="AC16VR_10510"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6011633.1"
                     /translation="MNTVPATAASVALDPLTQTLIDAAEACFVRHGVAKTTMSHVAEE
                     AGVSRTTLYKRFAVMEEVLQAVFIREFDRFEARLAGNLEPIDDPGERLVEIVVAIAEN
                     VPENAGLARLLDGSRTRTENMALAAGRRALNDRVIDMIGDPLDGLARAGVLRGDVERG
                     ELIEWIRRVVNSLAVFPQPRSRSSAERRRFVARFLLPSITAAHDLPPMEVC"
BASE COUNT          549 a          974 c         1082 g          545 t
ORIGIN      
        1 cgaggtacag ctcacccttc atgcgctcgg cgagcttgtg gggtgagtcg ggcttgtcgg
       61 tgacgatgcg ggtaccgtaa agcgacgcgg cagcggcgat ctctccggga aaggtgcccg
      121 ccgccgccac gacgaactgg ccgctcatgc agtagccgat gcagcccgtc ggcgatcgcg
      181 ccagagggtt gtcttccagg taggcgagca tcccgccggt atcacgcatg accgcctcca
      241 cggtgagccg cgagccgacg gcaaacatgc gcttgagctc ttcctcgccc ttgcgcaggt
      301 cgaagcggac gcggccctcc cgatagtaga gatcgggaag cagacagaag tagccttggc
      361 ccgcgatgcg ccggcagaag tcgcgcagct cttcgcgaat acccggcgcg tccatgtaaa
      421 ggatgacggc ggcaaacggg ccttcgccat cggggtgcgc ggcgaagcac tccatctcgc
      481 catccggagt ggccacattg acgtgaaatt cctgcatcgt gttctcgttt ttttcgcggg
      541 atgagggcaa cgagcataaa acgtgggtcg gaacctcaca atggcggtaa cgcgtggtaa
      601 ttccagacca agaaacccgg cccgccgcat tgacggcaag tgaacagatt tgataatttg
      661 ttcagattcg gccgctgcag ggatcaacca atgcatctcg atctcgtcat ccgtaacggt
      721 atgctcatcg atgggcgccg cactcccagc tatcgcgccg acgtcggcat ccgcgatggg
      781 aaggtcgccg tcatcgggcg gatagcgccc gggagcggag atcgcgagct cgatgccgac
      841 gggctcatcg tggcgcccgg cgtcgtcgat cttcataccc attacgacag ccagatcttc
      901 tgggatccct ggtgccggat ttccgggcag cacggagtca ccagcgtggt ggtgggtaac
      961 tgcgggttcg gcttcgcgcc ggttcggccc gagcatcgcg acagggccat gctcaccatg
     1021 gcgcgtaacg aagccgtgcc gctggagtgc atgcagcagg gcatgccttg ggactggacc
     1081 acctatccgg aatttctcga cagcctcgat cgcaccccga aaggggtgaa cgtgctctcc
     1141 tacgtggggc tcaacccgct gatggtgtgg ctcacgggct cggtggaaga ggcgaaaggc
     1201 cggccgctga cccgggcaga aaaggccgag gcggagcgcc tgcttgccga agccatggac
     1261 gccggcgcct gtggctggtc ggctcagttc atcggcgccg acaccgtcca gcgcgatcac
     1321 gacggcacgc ccatgatcac cgacctcatg agccgcgagg acatcgagtt cttcgccgcg
     1381 gcgctgggtc gtctgggccg cggcggcatt cagatgatcg gtccgccgaa gatggcggac
     1441 gtgcttgcgg cggcgtccgg gcgtccggtg atctacaacg tgctcgccgc tcgcagcgat
     1501 cagcacggct cgcccgcgga gtcttacaag gacgtaattg cgtggctcgc ccgggccaat
     1561 gccgagggaa accgcatcat cgctcatgcg gtgacctgcg ccatcgacta ccagttcacc
     1621 atggaggact ggaacctgtt cgactcgtcc ccgctgtggc gggagctcac catcggcacc
     1681 gtgcctgaaa gaaaggcgaa gatgatggac gcccagcgtc gtgctgcgct ccatgaggaa
     1741 tgggcggcgg gaaaggcgcc catcgcgggc ggtggaaccg agcagcgaga ggagcggatc
     1801 ggcgtcaagt ttcaggacct ggtactcgcc tacgtcaccg aagaaggcga gcacctgcgg
     1861 agctgggagg gccataccgt tgccgagata gcggcgggga tgggcgttca tcccatcgac
     1921 gcgttcctgg acgtatccat cgcctgcgac ctcaaggctg gctggcagag cgagccgagg
     1981 accatcgatc cccaggtcat gcgcgacatc gccaactgtc cgtatgccgt gcccgggctg
     2041 tccgacggcg gggcgcacac caagtttctg accacgggca cctacccgac ggagtttctc
     2101 gccacgtggg tgcgcgacaa cggggtgatg gacctggagg aagcgcactg gcgtctctcc
     2161 gcctactccg cgcagacggc ggggctgacg gaccgggggt tcatcgcgga gggcatgccc
     2221 gcggacatcg tggtctacga tctgcaggcg ctggagctgt tgccgtccga gcgcgttgcg
     2281 gattggcccg gcggcgcgtg gcacctcgac cggcgcgcga acggctggcg ctggacgatt
     2341 gtcaacggcg cggtcacctt tgcagacggc gtgtgcaccg gcgcgacgcc cgggcgcctg
     2401 ctcaggcacg gtcacgcatg aacaccgtgc cggccacggc ggcttcggtc gcgctggacc
     2461 cgctcacgca gaccctgatc gatgcagcgg aggcctgctt cgtgcgccac ggagtggcga
     2521 agacgaccat gtcgcacgtg gccgaggagg cgggcgtttc gcgcaccacg ctctacaagc
     2581 ggtttgccgt catggaggag gtgctgcagg cggtcttcat tcgcgagttc gatcggttcg
     2641 aggcgcggct tgcaggcaac ctcgaaccga ttgatgatcc cggtgagagg ctggtggaga
     2701 tcgtcgtggc gattgctgag aacgtgcccg aaaacgcggg gttggcccgc ttgctcgacg
     2761 gctcgaggac gagaacggag aacatggcgc ttgccgccgg gcggcgtgcg ctcaacgatc
     2821 gggtgatcga tatgatcggc gatcccctcg atgggcttgc gcgcgccggg gtgctgcgtg
     2881 gggacgtgga acggggcgag ctgatcgagt ggatccggcg tgtggtcaac tctttggcgg
     2941 tatttcccca gcccaggagc aggtcgtcgg ctgagcgccg gcggttcgtc gcccgctttc
     3001 tgcttccttc catcactgcc gctcacgatc tgccacccat ggaggtttgc tgatgtccgc
     3061 ttcgccgctc agaaacgtga cgacggaaga aatcgaatcc tttcaccgcg acggagccgt
     3121 tctggtcaag ggggtgctgt ccgacgagtg
//
LOCUS       DBNHQR010000464         7436 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1753371, whole genome shotgun sequence.
ACCESSION   DBNHQR010000464 DBNHQR010000000
VERSION     DBNHQR010000464.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7436)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7436)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7436
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1753371"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="AC16VR_10515"
     CDS             <1..822
                     /locus_tag="AC16VR_10515"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6011634.1"
                     /translation="PTYDAGLLTRDDMPAPVAAVLSRLESRIDEETMQGLNRRVASQG
                     EEPARVAADYLRETGLVSAADAVAVAGYERILGNTLVHLKLTGIALLLGCLVAVPLAL
                     VVARHQRIASGLLYVTGLVQTVPSLALLALLIPLVGLGQLPAIIALFLYSLLPIVRNT
                     LTGLFNVDPLLKEVAGSMGLTTAQQLRKVELPLAMPTILAGIKTAAIISIGTATLAAF
                     VGAGGLGEPIITGLTLNDHALILEGAVPAALLAVVVELLFELIERATVPAHLRVS"
     gene            964..2238
                     /gene="egtB"
                     /locus_tag="AC16VR_10520"
     CDS             964..2238
                     /gene="egtB"
                     /locus_tag="AC16VR_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019365043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HIR6011635.1"
                     /translation="MGKALAIDATAAAFSDTRRQSVDVCRPLEIEDYGLQAAVFASPP
                     KWHLAHTTWFFETFILKPFVSGYAPVEPIYEVLFNSYYNGIGEQHPRHQRGLLSRPTL
                     DEVLAYREHVDAAMAGCLELAGRADPQAIRQRTVLGIEHERQHQELFFTDLKYSLSVN
                     PLYPAYLAAELPVQSGTPVRWLGCEGGLVEIGHDGSGFCFDNELPRHRCFVEPFEIAS
                     RLVTNEEYQAFVDDDGYGRPELWLADGWTTLQSEGWKAPLHWLERDGQALEFTLHGLQ
                     PRRGSRPVCHVSGYEADAFARWANARLPTEQEWETVAAGHPAGAAGMDDGVYHPQAAP
                     ADEGPTQLYGACWQWTRSAYGPYPGYSASPGAIGEYNGKFMSNQWVLRGGSCVTKSSQ
                     ARPTYRNFFYPQDRWQFSGIRLARSVEGERRL"
     gene            2235..3209
                     /gene="egtD"
                     /locus_tag="AC16VR_10525"
     CDS             2235..3209
                     /gene="egtD"
                     /locus_tag="AC16VR_10525"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052706 - L-histidine
                     N(alpha)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HIR6011636.1"
                     /translation="MKQARRKARNVMFRDEHPDLGDSRREILAGLQRDQKAVNPKWFY
                     DERGSQLFDQITRLPEYYPTRTEVGILQENRREISACCGSGCLFIEPGSGSSEKVRIL
                     LETLKPSAYVPVDISAGFLEEAALKLGDEYPWLDIYAVCADFNRSWSYVDDLPEGKRV
                     VFYPGSTIGNLEPEAAVGFLRRVREITGDSGGVVIGVDTHKSTERLNAAYNDAAGVTA
                     DFNLNVLRRMNDLLDAEFDEGSFRHHAFYNTELKRIEMHLVSERAQAVRCNGATVEFA
                     EGETIHTENSYKYTVEDFAELVDAAGLELQQSWTDADELFSVHYLRVA"
     gene            complement(3225..3737)
                     /locus_tag="AC16VR_10530"
     CDS             complement(3225..3737)
                     /locus_tag="AC16VR_10530"
                     /inference="COORDINATES: protein motif:HMM:NF016162.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HIR6011637.1"
                     /translation="MWKFTGKSRPEFAEEPGPGQESVWDYPRPPALDPSDEQVEVRAD
                     GTPIASSRRTLRVLETAGPPTYYIPEQDIDWSQLTEIAQTTYCEWKGHASYYALASDP
                     ARVPVAWLYADPSDSFRAIDRHASFYPGRVECLVNGERVRPQPGEFYGGWITDRVVGP
                     FKGEPGTGHW"
     gene            3893..4414
                     /locus_tag="AC16VR_10535"
     CDS             3893..4414
                     /locus_tag="AC16VR_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011638.1"
                     /translation="MSIVELGAIGEFLGAFAVVITLVYLGLQVRHNTTSTRAGTLAYA
                     TDAWSDYLQRQSVDDLNLLLGLSIEPDELSSAEFMRAYYLYRVMFRRMESDFYQYRSG
                     ILDADTWDAYVESWRGDVFITPGGRAMWKLQRDYFGPEFRSAFDDVEASTPRTPQHLR
                     RRFAELWEAERPV"
     gene            complement(4476..5180)
                     /locus_tag="AC16VR_10540"
     CDS             complement(4476..5180)
                     /locus_tag="AC16VR_10540"
                     /inference="COORDINATES: protein motif:HMM:NF013223.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriorhodopsin"
                     /protein_id="HIR6011639.1"
                     /translation="MDKLFLQSGDFVGVTFWLVSIAMIAATVFFLYEGVKVKPQWRLS
                     LLVAGLVTLVAGVHYDYMRDYWVVEQATPIVYRYIDWLITVPLLMIEFYIILRAAGAA
                     LGHGSFVRLLVGTLVMLIFGYLGEAQVLNIHVGFWIGMVGWAIIIYEIFGGAAGKAAS
                     ASAQAVQSAFSAMRLIVLVGWAIYPIGYIWGYMLGGVDEGALNMVYNLADFVNKILFG
                     LIIWNLAIKDSGEAGH"
     gene            5326..5910
                     /locus_tag="AC16VR_10545"
     CDS             5326..5910
                     /locus_tag="AC16VR_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011640.1"
                     /translation="MGVFEYVAGFVAIVVGLAVARVMGGIGAFILSDRRAAMDWLLAG
                     WCLALIVTLIGWWMLAWFTLGQLETITYGAVFGWFAATALLYLASYILVPAGGAAFGG
                     AGAALGELRPAFFVCLAVHFAANIVESAVTGSLLPAQGLVVLMVVLSGIGAFLRGARA
                     HALLLLTWMVCMLVLNFVAVPAVGVNSGSVLEAG"
     gene            complement(5950..>7436)
                     /gene="xsc"
                     /locus_tag="AC16VR_10550"
     CDS             complement(5950..>7436)
                     /gene="xsc"
                     /locus_tag="AC16VR_10550"
                     /EC_number="2.3.3.15"
                     /inference="COORDINATES: protein motif:HMM:NF005713.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050487 - sulfoacetaldehyde
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0019529 - taurine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfoacetaldehyde acetyltransferase"
                     /protein_id="HIR6011641.1"
                     /translation="LLVTPQAANKTIGQGGFQEIEQMKLFEDMVCYQEEVRDPSRIAE
                     VLNRVIQNAKRRSAPAQINVPRDYWTQQVDIQLPAIVDFERPAGGEAAIAAAAKLLSE
                     AKFPVILNGAGVILADAIGDSAALAERLEAPVCCGYQHNDAFPGSHPLFAGPLGYNGS
                     KAGMELIAHADVVLALGTRLNPFSTLPGYGIDYWPKDAAIIQVDINPSRIGLTKPLSV
                     GIVGDAGDVARALLERVEEVSDEERDARRNLIARTKANWQQELAGMDHEDDDPGTTWN
                     ERARADKPDWMSPRMAWRAIRSVLPTEAIISSDIGNNCAIGNAYPTFETGRKYLAPGL
                     FGPCGYGLPSIIGAKIGCPDVPVVGFAGDGAFGISVTELTAIGREEWPAITMIVFRNY
                     QWGAEKRNSTLWFDDNFVGTELDEQVSYAGIAQACGLLGVQARTMDELSAALGEALDN
                     QMNHGRSTLIEAMINQELGEPFRRDAMKKPVEVAGIDPADMRRA"
BASE COUNT         1220 a         2345 c         2498 g         1373 t
ORIGIN      
        1 cccacctacg acgccgggct gctgacccgt gacgacatgc ccgcgcccgt ggccgccgtg
       61 ctgtcgcggc ttgaaagccg tatcgacgag gagaccatgc aggggctgaa ccggcgcgtg
      121 gccagccagg gcgaggagcc tgcccgcgta gcggccgact acctccggga aacggggctg
      181 gtgtcggcgg ctgatgccgt ggccgtggcc gggtacgagc ggatactggg caacaccctc
      241 gttcacctga agctcaccgg cattgcgctg cttttgggtt gcctcgtggc cgtgcccctg
      301 gcccttgtcg tcgcgcggca ccagcgcatc gccagcggcc tgctgtacgt gacgggcctg
      361 gtgcagaccg tgccttccct tgcgctgctg gcgttgctca tcccgctcgt tggcctgggc
      421 cagctgccgg ccatcatcgc gctgtttctc tactccctgc tgcccatcgt gcgcaacacg
      481 ctcaccgggc tgttcaacgt cgacccgctg ctcaaggagg tggccgggag catggggctg
      541 accacggcgc agcagctcag gaaggtggag ctgcccctgg ccatgcccac catcctcgcg
      601 ggcatcaaga ccgctgcgat catcagcatc gggacggcca ccctggccgc cttcgtaggc
      661 gccggcgggc tcggcgagcc gatcatcacc ggtctcacgc tgaacgatca cgcgctgatt
      721 ctggaaggcg cggttcctgc cgctttgctg gccgtcgtcg tggagctgct cttcgagctc
      781 atcgagcggg cgacggttcc cgcacatctg cgggtttcct gacgatgtcg tcaaagtccg
      841 gtccgaagct gttaggcttt agaggagtcg gaggcggcgg gcctatggcc tgaccccccg
      901 gaatggcgca cgctcagcgg cgcgcggctc ggatctgcgg gccgggaggg gcagacgatc
      961 aatatgggaa aggctttggc aatcgacgcg acggcggccg cgttcagcga tacccggcga
     1021 caatccgtgg acgtctgccg gccgttggag atcgaggact acggcctgca ggccgccgtg
     1081 ttcgcaagcc ctccgaagtg gcatctcgct cataccacct ggtttttcga gaccttcatc
     1141 ctcaagccgt tcgtctcggg ctacgcgccc gtcgagccca tctacgaggt gctcttcaac
     1201 tcttactaca acgggatcgg cgagcagcat ccgcggcatc agcgggggtt gctatcgcgc
     1261 cccacgctgg atgaggtgct cgcctaccgg gagcacgtgg acgcagcgat ggcaggctgt
     1321 ctggaactgg ccggacgggc cgatccgcag gccatacgcc agcgcaccgt tctgggcatc
     1381 gagcacgagc gccagcacca ggagctcttc ttcacggacc tcaaatactc gctgagcgtc
     1441 aacccgctgt acccggccta cctcgccgcc gagctgccgg tgcagagcgg gacgccggtg
     1501 cggtggcttg gctgcgaggg cggcctcgtc gagatcggcc atgacggaag cggcttctgc
     1561 ttcgacaacg agctgcctcg acatcgatgt ttcgtggagc ctttcgagat cgcctccagg
     1621 ctcgtcacca acgaggagta ccaggcgttc gtggacgacg acggctacgg gcgtccggag
     1681 ctgtggctgg ccgacggctg gacgacgttg cagagtgaag gatggaaagc gccgctgcat
     1741 tggctcgaac gtgacggcca ggcgctggag ttcaccctgc acggcctgca gccgaggcgg
     1801 gggtcgcggc ccgtctgtca tgtgagcggc tacgaggccg atgctttcgc ccgctgggcg
     1861 aacgcgcggc tgccgaccga gcaggagtgg gaaacggtgg cggccggaca tcccgccgga
     1921 gcggcgggca tggacgacgg cgtctaccac cctcaggcag ctcctgcaga tgaaggcccg
     1981 acgcagctgt acggcgcgtg ctggcagtgg acgcggagcg cgtacggtcc gtacccgggt
     2041 tacagcgctt cgcccggcgc catcggcgag tacaacggca agttcatgag caatcagtgg
     2101 gttctgcgag gaggctcctg cgtgaccaag tccagccagg cgaggccaac ttaccgcaat
     2161 tttttctatc cccaggatcg ctggcagttc agcggcatcc gcctggcccg ctccgtggag
     2221 ggcgaacgac ggttatgaag caggcgcgca gaaaggctcg aaacgtcatg ttccgggacg
     2281 agcatccgga tctcggcgac agccgccgcg agatattggc cggtctccag agagatcaga
     2341 aagcggtcaa cccgaagtgg ttctacgacg agcggggctc ccagctgttc gatcagatta
     2401 cccgcctgcc cgagtactac ccgacccgga cggaggtggg gatcctgcag gagaatcgtc
     2461 gcgagatcag cgcctgctgc ggcagcggtt gtctgttcat cgagcctggg agcggttcca
     2521 gcgagaaggt caggatcctg ctcgagactc tcaaaccgtc agcctacgtc cccgtggaca
     2581 tctccgccgg tttcctcgag gaagccgcct tgaagctcgg cgacgagtac ccctggctgg
     2641 acatctacgc ggtctgcgcg gatttcaatc ggagctggtc ctacgtggac gacctgccgg
     2701 aaggtaaacg ggtggttttc taccctgggt cgaccatcgg caaccttgaa cccgaggcgg
     2761 cggtggggtt tctgcgtcgg gttcgcgaga tcacgggcga ttcgggcggc gtcgtcatcg
     2821 gtgtggacac gcacaagtca accgaacgac tgaacgcggc gtacaacgat gcggccggcg
     2881 tcacggcgga cttcaacctg aacgtgctgc ggcggatgaa cgacctgctc gatgccgagt
     2941 tcgacgaggg gagctttcgc caccatgcgt tctacaacac cgagctgaag cggatcgaga
     3001 tgcacctggt gagcgagcgg gcccaagccg tgcgctgcaa cggtgccaca gtcgagtttg
     3061 ctgagggaga gaccatccac acggagaact cgtacaagta caccgtggag gactttgcgg
     3121 agctcgtgga cgcggccggc ctggagctgc agcagagctg gacggacgcc gacgagctct
     3181 tcagcgttca ctacctgaga gtggcctgac ccgaagcccg aggtctacca gtgcccggtg
     3241 ccgggttctc ccttgaacgg gccgaccacg cggtcggtga tccagccccc gtagaactcc
     3301 ccgggctggg gccttacccg ctcgccgttg accaagcact cgacccgccc cggatagaac
     3361 gaggcgtgcc ggtcgatggc ccggaaggag tcggaagggt cggcgtaaag ccacgcgacg
     3421 ggcaccctgg cggggtcgga tgcgagagcg taatagctgg cgtgaccctt ccactcgcag
     3481 taggtggttt gggcgatctc cgtcagctga ctccagtcga tgtcttgctc tgggatgtaa
     3541 taggtgggcg gccccgccgt ctccagaacc cgcagcgtcc tgcggcttga tgcgatcggc
     3601 gtcccgtcag ccctcacctc gacctgctcg tcgctcgggt ccagcgctgg tgggcgaggg
     3661 tagtcccaga cggactcctg gccgggtccc ggttcctcgg caaactccgg tctgctcttt
     3721 cccgtaaatt tccacatggc cgaggatact aacaggtgtt caacccggcc ggcatggggc
     3781 gctagcccgt cgacggcggt tcgtggtcga tgccgtgctt ccctggagat cgggactcgg
     3841 ccaaacgcct gagcttcgtt acgcttggca tgccgtaatt gaggagccat tcatgagcat
     3901 cgtagaactt ggcgcgattg gggagtttct gggcgcgttt gccgtggtca tcactctggt
     3961 ctacctgggt ctgcaggtcc gtcacaacac cacgtcgacc cgggcgggca cgctcgccta
     4021 cgcgacggac gcctggagcg actacctgca gagacagtcg gtggacgatc tgaacctgct
     4081 gctggggctt tcgatcgagc ccgacgagct gtccagcgcg gagttcatgc gcgcctacta
     4141 tctgtatcgc gtgatgttcc ggcggatgga aagcgacttc tatcagtatc gcagcggaat
     4201 cctcgatgcg gacacatggg atgcttacgt ggagagctgg cgcggcgacg tgttcatcac
     4261 gccgggcgga cgcgccatgt ggaaattgca gcgcgattat tttggaccgg agtttcgaag
     4321 cgctttcgat gacgtcgaag cgagcacgcc ccgcacgcct cagcacctgc gccgccggtt
     4381 cgcggagttg tgggaggctg agcggccggt ttgaccttct gctctcagcc gaacgggccc
     4441 caccccgggt ggggccctca ggtggctggg ctaactcagt gtccggcttc gcccgagtcc
     4501 ttgatggcga ggttccagat gatcagaccg aacaggatct tgttcacgaa gtcggccagg
     4561 ttgtacacca tgttcagcgc gccttcgtcc acgccgccga gcatgtagcc ccatatgtag
     4621 ccgatcgggt agatggccca tccaaccagc acgatcaggc gcatggcgct gaaagcggac
     4681 tgcaccgcct gcgccgaggc agatgccgct ttgccggcag ctccgccgaa tatctcgtag
     4741 atgatgatgg cccacccgac catgccgatc cagaagccga cgtgaatgtt gagcacctgg
     4801 gcttcgccga gatagccgaa gatgagcatc accagcgtgc cgacgagaag ccgcacgaac
     4861 gacccgtgtc ccagggctgc acccgccgcg cgcaggatga tgtagaactc gatcatcagc
     4921 agcggaacgg tgatcagcca gtcgatgtag cggtagacga tgggcgtcgc ctgctcgacg
     4981 acccagtagt cgcgcatgta gtcgtagtga acgccggcga ccagagtcac gaggccggcg
     5041 accagaagtg agagccgcca ctgcggtttg accttgacgc cctcgtaaag gaagaagacg
     5101 gtggcagcga tcatcgcgat agacaccagc cagaaggtga cgcctacgaa gtcgccgctc
     5161 tgcagaaaga gcttgtccat tgttaatccc tgtaaattgc gttattgctt gagttttact
     5221 gcatcgtgga tgtcaacagg gctcacgtga aagctgcgtg aacgggtctg tcatcactgg
     5281 tgatgctgga tcgggaaagg ccagggtgtg cgacggggag ttgccatggg cgtgttcgaa
     5341 tacgtagccg ggttcgttgc catcgtcgtc ggcctcgccg tcgcgcgggt catgggtggg
     5401 atcggcgctt tcattctgtc ggataggcgc gcggcgatgg actggctcct ggccggctgg
     5461 tgcctggcgc tgatcgtcac gctgatcgga tggtggatgc tggcctggtt caccctgggg
     5521 cagctggaga ccatcaccta cggcgccgta tttggttggt tcgcggcgac ggcgctgctc
     5581 tatctcgcga gctacattct ggttcccgct ggaggagcgg ctttcggcgg agccggtgcg
     5641 gccctgggcg aattgcggcc cgccttcttc gtctgcctgg ccgtgcactt tgctgccaac
     5701 atcgtcgagt ccgctgttac aggttctctc ttgccggctc aggggctggt cgtgttgatg
     5761 gtcgtgctct cgggaatcgg cgcgtttctt cgcggcgctc gggcacacgc cctgctgctg
     5821 ctgacctgga tggtctgcat gcttgtcctg aacttcgttg cggtcccggc cgtgggggta
     5881 aactcgggct cggtgctgga agcaggctga gccgaacgcg cccgctgccc ggccagcccc
     5941 gattccagcc taagcgcgtc gcatgtccgc gggatcgatg cctgcgacct caaccggctt
     6001 cttcatggcg tcgcgccgga agggctcgcc gagctcctgg ttgatcatgg cctcgatgag
     6061 cgtcgacctg ccgtggttca tctggttgtc cagcgcttca ccgagagcgg cggaaagctc
     6121 gtccatggtt ctggcttgga ctccgagcag cccgcaggcc tgggcgatgc cggcgtagct
     6181 cacctgctcg tcgagctccg tgccgacgaa gttgtcgtcg aaccacagcg tggagttgcg
     6241 cttctccgcc ccccactggt agttgcggaa gacgatcatg gtgatggccg gccactcctc
     6301 gcggccgatg gcggtgagct cggtgaccga gatgccgaag gcgccgtccc cggcgaagcc
     6361 caccaccggc acgtcggggc agccgatctt cgcgccgatg atcgaaggca ggccgtagcc
     6421 gcaggggccg aacaggccgg gcgcaagata ctttctgccg gtctcgaagg tggggtaggc
     6481 gttgccgatg gcgcagttgt tgccgatgtc cgagctgatg atggcttccg tgggcagcac
     6541 gctgcgtatc gcccgccagg ccatgcgggg gctcatccag tccggcttgt cggcgcgcgc
     6601 gcgctcgttc caggtggttc ccggatcgtc gtcctcgtga tccatgccgg ccagctcctg
     6661 ctgccagttg gccttggttc tggcgatcag gttgcgccgc gcgtcacgct cttcgtcgct
     6721 cacctcctcc acgcgttcca gcagggcccg ggcgacgtcg cccgcatcac ccacgatgcc
     6781 tacgctcagg ggcttggtga ggccgatgcg tgacggattg atgtcgacct gaatgatggc
     6841 ggcatccttc ggccagtagt cgatgccgta gccgggcagg gtcgagaacg ggttgagcct
     6901 cgtccccagg gcgagcacca cgtcggcgtg ggcaatgagc tccatgccgg ccttggatcc
     6961 gttgtacccc agcgggcccg cgaacagggg gtgcgagccg ggaaaggcat cgttgtgctg
     7021 gtagccgcag cacacgggag cctccaggcg ctcggccagg gcggcggaat cgccgatggc
     7081 atcggcaagg atcacgccgg cgccgttcag gatcaccggg aacttggcct ccgacagcag
     7141 cttcgccgcc gccgcgatgg cggcctcgcc gccggccggc cgctcgaagt ccacgatcgc
     7201 cggcagctgg atatcgacct gctgggtcca gtagtcgcgc ggcacgttga tctgcgccgg
     7261 agccgagcgg cgcttggcgt tctggatcac ccggttcagc acctcggcga tgcgcgaggg
     7321 gtcgcgcacc tcctcctggt agcagaccat gtcctcgaac agcttcatct gctcgatctc
     7381 ctggaagccg ccctggccga tggtcttgtt ggcggcctga ggggtgacca gcagca
//
LOCUS       DBNHQR010000465         2196 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1057761, whole genome shotgun sequence.
ACCESSION   DBNHQR010000465 DBNHQR010000000
VERSION     DBNHQR010000465.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1057761"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..364
                     /locus_tag="AC16VR_10555"
     CDS             83..364
                     /locus_tag="AC16VR_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011642.1"
                     /translation="MLERKSATSFSAVSVEPAGDVCCEAARRIAGDRMLVVDAPPLPL
                     DDCDRIMDCRCKYKKWSDVRQDERRSPIPNQFYFDNERRGRLPGRRALD"
     gene            complement(428..1249)
                     /gene="nei"
                     /locus_tag="AC16VR_10560"
     CDS             complement(428..1249)
                     /gene="nei"
                     /locus_tag="AC16VR_10560"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF007763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HIR6011643.1"
                     /translation="MPEGPEIRREADRIEAAIQGQRIEKVFFGLDRLSVWSSTLTGAT
                     VEAVGTRGKALLTTFDNGYTLYSHNQLYGKWMVRKRGTLPKTNRQLRVALHTANASAL
                     LYSASSIEVLPTDALHDYPPIARLGPDVLDTELEWRDVARRLRDPAFSGRALAALYLD
                     QHFMAGIGNYLRSEILHTAGVHPSVRPKELTTARIGQLARATLAISRRSYETGGITAS
                     KATVRRLRKQGMSRSGYRFSAFAREGLPCHRCGEPIARITANSRRLYYCRACQPR"
     gene            complement(1242..>2196)
                     /locus_tag="AC16VR_10565"
     CDS             complement(1242..>2196)
                     /locus_tag="AC16VR_10565"
                     /EC_number="1.6.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6011644.1"
                     /translation="GAGLAGCTCASALARHGLRVLILEKSGDIGGRLATRRRPKGHWN
                     HGAPALSPGDPAFTQFLGSLESAGTAQREDGPGGLPRFQGLPDMRELLRPLVARTPLA
                     FNTLARRLFRRGASWMVETEFGDQGPFRAVVVALPAPQATDLLARSRLDGLMPPAPVT
                     MAPVWTLLLGFDEQRPTPAPADRRIVERVIPQTHRPGDRPGWCAERWVVHATARWSSD
                     HVELDAADAGERLLRALAPSLADGDADRPDVVMTHRWRFAQTTSPMGRTHLWDPDTAV
                     GLAGDWCLGATAEDAFTSGGALARDISKCLLTDSAKAQGHA"
BASE COUNT          356 a          713 c          735 g          392 t
ORIGIN      
        1 attcaattgc atttttacga cagaccgggg ctggtttcgt tgatagttta ggggtccaac
       61 aagctaaaaa cttccttgac tcatgctgga acgcaaatct gccacctctt tttccgcggt
      121 ctcggtggaa cctgccggcg acgtctgctg tgaggcggca cgtcgaatcg caggggatcg
      181 tatgctggtc gtcgacgcgc cgccgctgcc gcttgatgat tgcgaccgga tcatggactg
      241 ccgatgtaag tacaagaagt ggagtgatgt ccgtcaggac gaacgacgct cgccgatccc
      301 caaccagttt tacttcgata atgagcgccg tggccgcctc ccgggacgcc gcgcgctcga
      361 ctgacagggc gcgctgtgtt ggctcacctc gtcaacttgt ccgcccgcaa cacgcctgcc
      421 cgttggctca acggggctga catgctcggc agtagtacag ccgccgggag ttcgcggtga
      481 tccgagcaat gggctcgccg caacgatggc atggcagccc ctcccgcgcg aaagcgctga
      541 agcggtagcc cgatcgcgac attccttgct ttcttaagcg cctgaccgtt gccttgctcg
      601 cggtgatgcc acccgtctcg tacgaccgcc ggctgatggc cagggtggcg cgtgccagct
      661 ggcctatgcg ggcagtggtc agctccttcg gcctgaccga cgggtgcacg ccggccgtgt
      721 gcagaatctc cgaacgcagg tagttgccga tcccggccat gaagtgctga tccaggtaca
      781 gggcggccag ggcgcgcccc gagaatgcgg gatctcgcag acgtcgggcg acgtctcgcc
      841 actcgagctc cgtgtcgagc acgtccggtc cgagccgggc aatgggcggg taatcgtgca
      901 acgcgtcggt aggcagaacc tcgatgctcg atgcgctgta aaggagggcg ctggcgttgg
      961 ccgtgtgcag cgcgaccctg agttgccggt ttgtcttcgg aagcgtgccc cgctttctga
     1021 ccatccactt gccgtacaac tggttgtgtg agtagagcgt gtagccgttg tcgaacgtgg
     1081 tcagcagagc cttgccccgg gtgcccacgg cctcgaccgt tgctccggtg agcgtgctgg
     1141 accatacaga caggcgatca agaccgaaga agaccttttc gattctctgc ccctggatcg
     1201 ctgcctcgat ccggtcggct tcgcggcgaa tttcaggtcc ttcaggcatg gccttgcgcc
     1261 ttggcgctgt cagtcagcag gcatttggag atgtcccggg caagcgcgcc gccgctggta
     1321 aaggcgtcct ccgccgtcgc tcccaggcac cagtcccccg ccagacccac cgctgtgtct
     1381 ggatcccaca ggtgggttct gcccatgggt gacgtggtct gcgcaaaccg ccaccggtgt
     1441 gtcatgacca cgtcgggtcg atccgcgtct ccgtcagcca gcgagggggc gagggcccga
     1501 aggagcctct cgccggcgtc cgcagcatcc agttcgacgt ggtcgctcga ccaccgggcc
     1561 gttgcgtgca cgacccagcg ctccgcgcac catccgggcc gatcgccggg gcgatgggtc
     1621 tgtggaatca cccgctcgac gatacggcgg tcggcagggg ccggcgtcgg ccgttgctcg
     1681 tcgaatccga gcagcagcgt ccacaccggc gccattgtga caggcgctgg cggcatcaag
     1741 ccgtcaagtc gacttcgcgc aaggaggtcg gtagcctggg gtgccggcag ggcgacgacc
     1801 accgctcgaa aagggccttg atccccgaac tcggtttcga ccatccagct ggctccgcgc
     1861 cgaaacaggc ggcgcgcgag ggtgttgaac gcgagtggcg tgcgggcgac gagcgggcgc
     1921 agcagctcgc gcatgtccgg caggccttgg aagcggggca gccccccggg accgtcctcg
     1981 cgctgcgcgg tcccggcaga ctccagagac ccaaggaact gcgtgaaggc cggatccccg
     2041 ggagagagcg caggcgcgcc atgattccaa tggcccttgg ggcgccgtcg ggtggccagg
     2101 cggccgccga tgtcaccgct cttttccagg atcaacacgc gcaggccgtg cctggccagg
     2161 gcagatgcgc aggtgcagcc ggccaggccg gctccg
//
LOCUS       DBNHQR010000466         2529 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_867118, whole genome shotgun sequence.
ACCESSION   DBNHQR010000466 DBNHQR010000000
VERSION     DBNHQR010000466.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_867118"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="AC16VR_10570"
     CDS             <1..505
                     /locus_tag="AC16VR_10570"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6011645.1"
                     /translation="EEARYLELSPVDGQIDILASDRLPRLPLVGNGYVVPGAGGLVVG
                     TTYEYRPWPAGSATAANLRQLPEDVGRAARWLGRARGTRAVTSDRLPVAGRLFDLNAH
                     PLTNAYATTGHGSMGTVFAPFAGAVVASLISGDFSPLERDLEQALSSARFRTRQARRG
                     YRHGAQP"
     gene            complement(511..1689)
                     /locus_tag="AC16VR_10575"
     CDS             complement(511..1689)
                     /locus_tag="AC16VR_10575"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HIR6011646.1"
                     /translation="MYKIRTLNNIAVRGLERFSREHYEVGSEIADPHAILLRSHQLAP
                     TDLNESLRAVARAGAGVNNVPVADCTEKGIVVFNTPGANANSVKELVLAALLISARDV
                     VGGIEFVSSLGDIEDEDELDRLVEAEKKRFKGRELQGKSLGVVGLGAIGSLVARAALD
                     LGMEVLGYDPAISVDAAWRLPSQVRRMENLPSLFARADYVTLHVPLLPETTGMINDES
                     LRLFRPGSVLLNFARQPIVDSGALRAALDDGRVALYVADFPVPGLTDHPNVRLTPHLG
                     ASTEEAEENCAVMAAEQLIEFLETGNIRNSVNFPSVNLEPSKGYRLAITNRNVPGMLG
                     KMTSILAESNINVIDMLNKSRDEVAYNLIDVAEEPAPELIDAICAIESVMNVREFKID
                     "
     gene            complement(1779..>2529)
                     /locus_tag="AC16VR_10580"
     CDS             complement(1779..>2529)
                     /locus_tag="AC16VR_10580"
                     /inference="COORDINATES: protein motif:HMM:NF016027.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR6011647.1"
                     /translation="VFAAWLRRVPVVAHESDLTPGLANRLALPFLRTVCTSFSSTAFP
                     GFKGRVVHTGTPLREALLAGDRARGRQRLGAGKDERVLLITGGSLGADVINQVVREVL
                     DELLASWLVVHVCGPGKVTGTAQPRYLQFEYVTEGWGDILTAADLVVSRAGANTLYEL
                     LALGKPNLLIPLSRRASRGDQLENAEFALAQGYSRVLLEEDLDGPALLREIAALDAQR
                     EAAAARLHGFAPPDAVELICSELTPFLPAGS"
BASE COUNT          404 a          875 c          828 g          422 t
ORIGIN      
        1 cgaagaagcc cggtacctgg agttatcgcc cgtcgacggc cagatcgaca tcctggcatc
       61 cgaccgcctt cccaggttgc ctttggtggg caacggctac gtggtgcccg gcgccggcgg
      121 gctcgtcgtg ggcacgacgt acgagtaccg gccctggccg gccggttccg ccaccgcagc
      181 aaacctgcgt cagctgccgg aggacgtcgg gcgcgcggcc cgctggctgg gtcgtgcgcg
      241 aggaacccgc gcggtgactt cggaccggct gccggtggcc gggcgcctgt tcgacctgaa
      301 cgcgcacccc ctgactaacg cgtacgcgac caccggtcat ggctccatgg ggacggtgtt
      361 cgcgcctttc gccggcgccg tcgtcgcctc gctgatcagc ggcgacttct ccccgctgga
      421 gcgggatctg gagcaggccc tgtcgtccgc gcggttccgc acgcgccagg cgcggcgtgg
      481 ctatcggcac ggggcgcagc cttaggccgg ctagtcgatc ttgaactcgc gcacgttcat
      541 gacgctctcg atggcgcaga tggcatcgat gagctcgggc gccggctcct cggcaacgtc
      601 gatcaggttg taggccactt cgtcccggct cttgttgagc atgtcgatga cgttgatgtt
      661 cgactccgcc aggatcgacg tcatcttgcc cagcatgccc ggcacgttgc ggttcgtgat
      721 cgccagccga tagcccttgc tgggctccag attcacggac gggaagttga ccgagttgcg
      781 gatgttgccg gtttccagaa actcgatgag ctgctccgcg gccatcaccg cgcagttctc
      841 ctctgcctcc tcggtgctcg ccccgagatg aggggtgagc ctgacgttgg ggtggtcggt
      901 cagccccggg accgggaaat cggccacgta gagggcgacc cggccatcat ccagcgctgc
      961 gcgcagggcc cccgagtcaa cgatgggctg tcgggcaaag ttcagcagca cgcttcccgg
     1021 ccggaagagc ctgaggctct cgtcgttgat catccccgtg gtttccggaa gcagcggaac
     1081 gtgcagggtc acgtagtcgg cccgcgcaaa caggctcggc aaattctcca ttcggcggac
     1141 ctggcttggc agacgccagg cggcatcgac cgagatggcc gggtcgtagc ccagcacctc
     1201 catgccgaga tcgagggccg cacgagccac cagcgagccg atggcgccaa gccccacgac
     1261 gccgagtgac ttgccctgca gctcgcgccc cttgaaccgc tttttctcgg cttcgacaag
     1321 ccgatcgagc tcgtcttcgt cctcgatatc gcccagcgag ctgacgaact cgatgccgcc
     1381 caccacgtcc cgcgcggaga tcagcagagc ggccagaacc agctccttga ccgagttcgc
     1441 gttggcaccg ggcgtgttga acaccacgat gcccttttcc gtgcagtccg ccaccgggac
     1501 gttattgacc ccggcacccg cccgcgctac cgcgcgcagg ctctcgttga ggtcggtcgg
     1561 ggccagctgg tggctgcgca gaagaatggc gtgcgggtcc gcgatctccg agcccacctc
     1621 gtagtgctcg cgggaaaatc tttccagccc gcgaacggca atgttgttta atgtccgaat
     1681 cttgtacata cgctgcagcc gctgtcccgt tgcccatcac ccccggaggc ggggggcgcg
     1741 tatggtatac aaatcctgtt gccgcgcgga aggtccgatc aggatccggc cggaagaaag
     1801 ggggtcagct ccgagcagat cagctccacc gcgtcaggcg gcgcaaagcc atgcagccgg
     1861 gccgccgcgg cttcacgttg cgcatccagc gcggcgatct ctcgcagcag cgcggggccg
     1921 tccagatctt cctccagcag cacccggctg taaccctggg ccagggcaaa ctcggcgttc
     1981 tccagctgat cgcccctgct ggcccgccgg gaaaggggga tcagcagatt cggcttgccg
     2041 agagccagca gctcatagag cgtgttggcc cccgcacggg agaccaccag gtctgcggcc
     2101 gtcaggatgt cgccccagcc ctcggtgacg tactcgaact gcaggtagcg cggttgcgct
     2161 gtgcccgtga cctttcccgg cccacacacg tggaccacca gccagctcgc cagcagctcg
     2221 tcgagcacct cgcgaaccac ctgattgatg acgtccgcgc cgaggctgcc cccggtgatc
     2281 agcagcacgc gctcgtcttt tcccgccccg agccgctgcc ggccccgggc gcggtccccg
     2341 gccaggagcg cttcccgcag gggtgtcccc gtatgcacca cccgcccttt gaagccggga
     2401 aacgccgtcg aggaaaagct ggtgcagacg gttctaagga acggcagggc caggcggttg
     2461 gccagaccgg gcgtgaggtc ggattcgtgg gcaacgacgg gcacgcgccg cagccaggcg
     2521 gcgaaaacc
//
LOCUS       DBNHQR010000467         2141 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1247691, whole genome shotgun sequence.
ACCESSION   DBNHQR010000467 DBNHQR010000000
VERSION     DBNHQR010000467.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1247691"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..1182)
                     /locus_tag="AC16VR_10585"
     CDS             complement(7..1182)
                     /locus_tag="AC16VR_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007351880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6011648.1"
                     /translation="MSGPLTGVRVLDLTSMVSGPVATMLLGDQGADVIKVEPLTGDLV
                     RYMGPNRDGLTSGFISSNRNKRSIAVDLKQEAGMDVVKRLAATSDVFVQNFRPGAIER
                     MGLGEDVVREIRPDIVYVSISGFGETGPYAHKRVYDPVIQALSGLAAIQRDRETGRPK
                     MVRTIIPDKTTALTAAQAITAALFARERSGEGQHVKLAMLDTMVAYLWPEGMAGFTFI
                     GKEVKAARAQFAQDLIFATTDGFITAGAVSDAEWQGMCRALEREEWIDDERFNTPNGR
                     IVNVQERLRLTAEVLETRSSAEWLARLDAEGVPCAPVLDRHEVFEHEQVRINEMVEEL
                     DHPVAGRIRQPRPAARFDRTPAGIHRHAPMLGEHTDELLQEMGLDADALRAAGVIGG"
     gene            complement(1192..>2141)
                     /locus_tag="AC16VR_10590"
     CDS             complement(1192..>2141)
                     /locus_tag="AC16VR_10590"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding enzyme"
                     /protein_id="HIR6011649.1"
                     /translation="VVACPLMHGTGMWLGSLAPLLTGGTVITTPQLGLDPDLIWTLVG
                     SHRATDLVVVGDAFARPLLAALDEAERRGEPYDLSSLRQIVSSGVMWSREVKEGLLRH
                     RDMMLVDAMGSTEGSMGSSVSSRASGPTTAKFQLAEGVKVFTDEGREVEPGSGEIGKL
                     ATSGFVPLGYYKDPVKSAETFREVDGVRYSFPGDYATVEADGSITLLGRGSVCINTAG
                     EKVFPEEVEEAVKRHPAVVDCLVVGIPDERFGERVVAVASFHDDQDVSEPELIEFARD
                     HLAGYKLPKQVLRVPQVQRAPNGKADYKWAKSTALAELT"
BASE COUNT          349 a          756 c          662 g          374 t
ORIGIN      
        1 ggcggatcag ccgccgatca ccccggcggc cctgagcgcg tccgcgtcca gacccatttc
       61 ctgcagcagc tcgtcggtat gctcgccgag catcggcgcg tgccggtgga tgccggccgg
      121 cgtcctgtcg aaccgcgccg ccggtctcgg ctgacgaatg cggccggcca ccgggtggtc
      181 cagctcttcg accatctcgt tgatcctcac ctgctcgtgc tcgaagacct cgtggcgatc
      241 cagcacgggg gcgcagggaa cgccttcggc atcgagtcgg gccagccact ccgcgctgct
      301 gcgcgtctcc agcacctcgg cagtcaggcg cagccgctcc tgcacgttga cgatccggcc
      361 gttgggcgtg ttgaaccgct cgtcatcaat ccactcctca cgctctagcg cgcggcacat
      421 accctgccac tccgcatcgg acaccgcgcc tgccgtgatg aagccgtcgg tggtggcaaa
      481 gatgaggtcc tgagcgaact gggcccgcgc agctttcact tccttgccga tgaacgtaaa
      541 gcccgccatg ccctccggcc agagataggc caccatggtg tcgagcatgg ctagcttcac
      601 atgctgacct tcgccgctgc gctcccgggc gaagagggcc gcggtgatgg cctgggccgc
      661 ggtaagcgcc gtcgtcttgt ccggaatgat ggtgcgcacc attttcggcc ttccggtttc
      721 ccggtcgcgt tggatcgcgg caagaccgga aagcgcctga atcaccgggt cgtacacccg
      781 cttgtgcgcg taagggcccg tctctccgaa tccgctgatg gacacgtaga cgatgtccgg
      841 gcggatctcc cgcaccacgt cctctcccag acccatgcgc tcgatggccc caggccggaa
      901 gttctgaacg aagacgtcgg aagtggcagc caggcgcttg accacgtcca tgccggcctc
      961 ctgcttgagg tccaccgcga tgctccgctt gttgcggttc gagctgatga acccggacgt
     1021 gagaccatcc cggttgggcc ccatgtagcg gacgagatcg cccgtcaacg gctcgacctt
     1081 gatgacgtcg gcaccctgat caccgagcag catggtggcc accgggccgg aaaccatgct
     1141 ggtcaggtcg aggacccgga ctccagttag cggtccgctc atatcaacct ctcaagtcag
     1201 ttccgcgagc gccgtgcttt tcgcccactt gtagtccgcc ttgccgttgg gcgcgcgctg
     1261 cacttgcggc acccgcagaa cctgcttggg aagcttgtag cctgcaaggt ggtcgcgggc
     1321 aaactcgatc aattcgggct cggatacgtc ctggtcgtcg tgaaaggacg cgaccgccac
     1381 cacgcgctcg ccaaaacgct catcgggaat gccgacgacc aggcaatcga cgacggccgg
     1441 atggcgcttg acggcctctt cgacctcctc ggggaacacc ttctcgccgg ccgtgttgat
     1501 gcagacgctg ccgcgcccga gcagcgtgat gctgccgtcc gcttccacgg tggcgtagtc
     1561 gccgggaaag ctgtaccgaa cgccgtccac ctcgcggaac gtttccgcag atttgaccgg
     1621 atccttgtag tagccgaggg gcacgaagcc gctggtggcg agcttaccga tctcgcccga
     1681 acccggctca acctcgcggc cctcgtcagt gaagactttc accccttcgg cgagctgaaa
     1741 cttggccgtg gtcggccccg acgcccggga gctgacggag ctgcccatgc ttccttccgt
     1801 cgagcccatg gcgtccacga gcatcatgtc ccgatgacgc agcagccctt ccttgacctc
     1861 tctgctccac atcaccccgc tggagacgat ctgccgcagg gaactgaggt cgtaaggctc
     1921 gccgcgacgc tcggcctcgt cgagcgcggc cagcagcgga cgcgcgaaag cgtccccgac
     1981 aacaacgaga tccgtcgccc ggtggctgcc caccagggtc catatcagat cgggatccag
     2041 ccccagctgc ggcgtcgtga tcaccgtgcc gccggtgagc agcggcgcga ggctgcccag
     2101 ccacatgccc gtgccgtgca tcagcgggca ggcgaccacc g
//
LOCUS       DBNHQR010000468         4840 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1121413, whole genome shotgun sequence.
ACCESSION   DBNHQR010000468 DBNHQR010000000
VERSION     DBNHQR010000468.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4840)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4840)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4840
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1121413"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(312..1703)
                     /locus_tag="AC16VR_10595"
     CDS             complement(312..1703)
                     /locus_tag="AC16VR_10595"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6011650.1"
                     /translation="MVEHASTDSAGRDAVPPLSRRTLFYYGLADMPIQMAAVPVAAFV
                     PNYYGQDLGVSLAAVGLVLLLSRSFDAITDPLIGWLSDRTDTRWGRRRVWMLASVPIL
                     MLAIYKLFMPDPGDASAVYLLIWLVVLWLGWTMLFIPYYAWAAELSPEYNERSRITGW
                     RSSIGLAANVLSKLVPVVAIFCCAYGGTQETLFLVGTMTLILLPLTVGLTVAMVPEGK
                     DFIPARMPLVPGLKIMWRNGPFKRLVAAFFVNQLGTSISTALIVFYVRSVLQDDDKTI
                     LQLLVYYGFNLCGIPFFVRYSGIVGKHRAWCIALLLFAFFMLGYLLLGAGDFYWMLPI
                     TAITGFLAGSFWVIPNSMKADVIDIDRLESGEDRTAWYFAVWSLATKVALSVGPAIAL
                     GLLSLTGYVPAAEAGAYEGSTLGLKLLFVFGPSTGLLLTALIAWRYPLTETKHRELRQ
                     ALARSRSRPRPAG"
     gene            complement(1687..3087)
                     /locus_tag="AC16VR_10600"
     CDS             complement(1687..3087)
                     /locus_tag="AC16VR_10600"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6011651.1"
                     /translation="MASDQASGASARPDAAHSGPARPLSRRTLFYYGLADMPIQMAGI
                     PVAAFIPNSYGQDLGVSLGAVGTIWLLTRLFDAVSDPLIGWLSDRTDTRWGRRRVWMV
                     AAVPILMLSVYKLFMPDPAAVSAGYLLGWLVALWVGWTMLFIPYYAWAAELSPDYNER
                     TRITGWRSWIGMAANVVSKVVPVIAIYCCAFGGTEETLFLIGTMTLILLPVTVGLTVM
                     KVPEGRDYLPTRIPLARGLKLMWQNAPFKRLVLAFFINQLGSSISTALVVFYIRVVLQ
                     EDAMPIVFLLTYYLFNLSGIPLWVWFSGKVGKHRAWCVGLALFGTAQCGYLLLGPGDF
                     LYMLPIGALTGFLGGSFWVLPNSMKADVIDVDTLNTGEDRAAWYFAVWSFTTKVGQSI
                     GPWLALTLLAFVGYDPASQPEPTAGGLLGLKLLYVFGPACGFWLAALIAWNHPLTESR
                     HREIRASLEKVRGRAR"
     gene            3231..>4840
                     /locus_tag="AC16VR_10605"
     CDS             3231..>4840
                     /locus_tag="AC16VR_10605"
                     /inference="COORDINATES: protein motif:HMM:NF013199.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR6011652.1"
                     /translation="MKARAFVAAILVLTGASAGARENVAWPAYGGGPGGGHYSQADQI
                     TPDNVAGLEVAWQHRSGDYREGTIRGVEGFSGDAVAASAFIGTPIVVNDTLYYCTPFN
                     RVFALDPQTGAERWMFDPGVKHELEHLTNCRGVSSWTGPLASPDALCAHRIIAPTLDG
                     RIFAVDGKTGKRCEGFGRNGEIDLTEGLTEHGAQEYGITSAPAILNDILVTGSYVTDS
                     IRIDIPSGVVRAYDLRTGAFRWGWNPVPPGTAERDEAGNYRAGTTNVWSTISVDAERN
                     LVIVPTGNSSPDYYGGHRDGDLDYYSSSVVALDGDTGEVVWHYQTVHHDIWDYDLPAQ
                     PTLLDVTIDGKTRPAVVQVTKMGMTFVLDRETGQPLFPVEERPVPQEGAAPGEYLAPT
                     QPFPLKPEPLHKLGISPDDAWGFTFLDRGWCRDALEAMTTGPIYTPISVNKETPLYPS
                     VIGGQNWGAPAVDPERQIMVANTHHVAMILNLIPRDQCDGTENFPQEGSPYCATLRPF
                     LSRFGAPCTKPPWGTLSAVNLSTGDIEW"
BASE COUNT          942 a         1553 c         1613 g          732 t
ORIGIN      
        1 tgatctgcgc cacgacgcga tcgcgatcgg tcaccagaac ggtttcgccg cccgctgcca
       61 ggcgcacgta ctcgctcagc ttgttcttga gtactttgat gcctacggcc ttcatttcac
      121 aaaagtagct actagtagct accaatgcaa ccgggatttt cgtctgcccg aaaaaactac
      181 tcgagcgcgg ctccctgcgg aaccggcagc gcttgtacga gtatgtagtc tgtcgccgga
      241 ggcgagcggc agccgaagcc gcctctagcc tctccaccgc tggacggggc ctttacagga
      301 gcgcgctggg ttcaacccgc cggccgcggc ctgctgcgcg agcgggcgag agcctgtctc
      361 agttcccggt gcttggtctc ggtgagcggg tagcgccacg cgatcagcgc ggtgaggagg
      421 agcccggtgg acggcccgaa gacgaacagc agcttgagcc ccagcgtcga gccttcgtaa
      481 gcgcctgcct cggcagccgg cacgtagccg gtgagggaga gcagccccag agcaatagcc
      541 ggcccgactg acagggcgac cttggtggcc agggaccaga cggcgaagta ccaggcggtg
      601 cggtcctcgc cgctctccag ccggtcgatg tcgatcacgt cggccttcat ggagttgggg
      661 atcacccaga aagacccggc gagaaaaccc gtgatcgccg tgatgggcag catccagtag
      721 aagtcgccgg ctcccagcag caggtagccc agcatgaaga aggcgaacag caacagagcg
      781 atgcaccagg ctcgatgctt gccgacgatg ccggagtagc gcacgaaaaa cgggatgccg
      841 cagaggttga acccgtagta caccagcagc tgcaggatgg tcttgtcgtc gtcctgcagc
      901 acgctgcgca cgtagaagac gatgagcgcc gtggagatgg aggtccccag ctggttgacg
      961 aagaacgcgg cgaccagacg tttgaagggc ccattgcgcc acatgatctt cagccccggc
     1021 accaggggca tgcgggccgg gatgaagtcc ttgccttcgg gaaccatggc caccgtcagc
     1081 cccacggtca gtggcaaaag aatcagcgtc atggtgccga cgaggaacag ggtctcctgg
     1141 gtgcccccgt aggcacagca gaagatggcc accacgggta ccagcttgct gagcacgttg
     1201 gcggcgagcc cgatgctgga ccgccagccc gtgatgcggg aacgctcgtt gtactccgga
     1261 gacagctcgg ccgcccaggc gtagtaggga atgaacagca ttgtccagcc gagccacaga
     1321 accaccagcc agatcagcag gtagacggcg gaggcgtcgc cgggatcagg catgaagagc
     1381 ttgtagatgg cgagcatcag gataggcacg gaagccagca tccagacccg gcgacggccc
     1441 cagcgcgtat ccgtgcggtc cgacagccag ccgatcagcg gatcggtgat ggcgtcgaac
     1501 gatcgcgaga gcaggagcac cagcccgacg gccgccaggc tcacgcccag atcctggccg
     1561 tagtagttgg gcacgaaggc ggccaccggc accgcggcca tctggatggg catgtcggcc
     1621 aggccgtagt agaacagcgt acgccgcgaa agcggcggca cggcgtcccg gccggctgag
     1681 tcggtgctag cgtgctcgac cacggacctt ctccaggctg gcgcgtatct cccggtggcg
     1741 cgactcggtg agcggatgat tccaggcaat gagggcagcc agccagaagc cgcaggccgg
     1801 cccgaacacg taaagcagct tgagtcccag caggccaccc gccgtgggct ccggctggct
     1861 ggccggatcg tagcccacga aggccagaag cgtcagcgcg agccaggggc cgatggattg
     1921 cccgaccttg gtggtaaagg accagacggc gaagtaccac gccgcgcggt cctcgccggt
     1981 gttgagcgtg tccacgtcga tcacgtccgc cttcatggag ttcgggagca cccagaaaga
     2041 gccaccaaga aacccggtga gcgcgccgat gggaagcatg tagaggaagt cgccgggacc
     2101 cagcagaaga tagccgcatt gggcagtgcc gaacagggcc aggccgacgc accaggcgcg
     2161 atgcttgccc accttgcccg agaaccagac ccacagcgga atgccgctca agttgaacag
     2221 gtagtaggtg agcagaaaca cgattggcat ggcgtcctcc tgcaacacca cgcgaatgta
     2281 gaacaccacc agcgccgtgg agatggacga gcccagctgg ttgatgaaga acgccagcac
     2341 caggcgcttg aacggagcgt tctgccacat gagcttgagc ccccgcgcca ggggaatacg
     2401 ggtgggcagg tagtcgcgac cttcggggac cttcatcacg gtcaggccca cggtgacggg
     2461 cagcaggatc agggtcatgg tgccgatgag aaacagcgtc tcctcggtgc cgccgaacgc
     2521 gcagcagtag atggcgatca cgggaaccac cttgctgacc acgttggccg ccatgccgat
     2581 ccaggaacgc cagcccgtga tgcgggtgcg ctcgttgtag tccggagaca gctcggccgc
     2641 ccaggcgtag taggggatga acagcatggt ccagcccacc cagagcgcca ccagccagcc
     2701 cagcagatag cctgcgctca ccgcagcagg atccggcatg aagagcttgt agaccgacag
     2761 catgagtatg ggcactgctg ccaccatcca aacgcgtcgg cgcccccacc gcgtatccgt
     2821 gcggtcggac agccagccga tcagcggatc cgataccgca tcgaacaggc gcgtgagaag
     2881 ccagatggtc ccgaccgccc cgaggctgac gccgaggtcc tggccgtagg agttggggat
     2941 gaaggcggcc acgggaatgc ccgccatctg gatgggcatg tcggcgagcc cgtagtagaa
     3001 cagggttctg cgcgacagcg gccgcgccgg gccggagtgc gcggcatcag gccgtgccga
     3061 tgcgccggac gcctggtcgc tggccaaatg gatctcccct caccctgaaa agcggatgca
     3121 ccacgtcccg ccgagtataa cgtgttttcc ttttgatctg cggcggagcg ggtggttgaa
     3181 tgaggctcag cgcaggctgg agccgcgcga tgatcgggag gggattcacc atgaaagcca
     3241 gagctttcgt cgctgccatt ctggtgctta cgggcgcatc cgcgggtgct cgggagaacg
     3301 tcgcctggcc ggcctacggt ggcggacccg gaggcggtca ctactcccag gccgaccaga
     3361 tcacgccgga caacgtggcg ggcctggagg tcgcctggca gcatcgctcg ggcgactatc
     3421 gagagggcac catccgcggc gtggagggct tcagcggcga cgcggtggcc gcctcggcct
     3481 tcatcggcac gcccatcgtc gtgaacgaca cgctgtatta ctgcacgccg ttcaatcggg
     3541 tttttgccct ggatcctcag acgggcgccg agcgttggat gttcgacccg ggcgtgaagc
     3601 acgagctgga gcacctcacc aactgccgcg gggtgagctc ctggacgggc cccctggcgt
     3661 ctcccgacgc cctttgcgcc caccgcatca tcgccccgac cctggacggc cgtatcttcg
     3721 ccgtggacgg aaaaacgggt aagcgttgcg aaggcttcgg ccgcaacggc gagatcgatc
     3781 tcaccgaggg gttgaccgag catggcgccc aggaatacgg cattaccagc gcgccagcca
     3841 tactcaacga cattctcgtc acgggctcct acgtcacgga cagcatccgc atcgacatac
     3901 cctcgggcgt cgtgcgtgcc tatgacctgc gcaccggagc gttccgctgg ggttggaacc
     3961 ccgtaccgcc gggcaccgcc gagcgcgacg aggcggggaa ctatcgcgcg ggcaccacca
     4021 acgtctggtc caccatcagc gtggacgccg aacgcaacct ggtgatcgtg ccgacgggca
     4081 actcgtcgcc cgactactac ggcggccacc gggacggcga cctggactac tactcgagct
     4141 ccgtggtggc gctggacggc gacaccggcg aggtcgtctg gcactaccag acggtgcatc
     4201 acgacatctg ggattacgac ctgcccgcgc agccgaccct gctggatgtc accatcgacg
     4261 gaaagacgcg tccggccgtg gtgcaggtga ccaagatggg tatgacgttc gtgctcgatc
     4321 gggagaccgg gcagccgctc tttcccgtgg aggagcggcc ggtgcctcag gagggtgcgg
     4381 cgcccggcga gtatcttgcg cctacccagc ccttccccct caagcccgag ccgctgcaca
     4441 agctgggcat ctcccccgac gatgcgtggg gcttcacgtt cctggacagg ggctggtgtc
     4501 gggatgcgct cgaggccatg accaccgggc cgatctacac gccgatctcc gtgaacaagg
     4561 aaaccccgct gtacccgtcg gtgatcggcg gtcagaactg gggcgcgccg gcggtggatc
     4621 cggagcgtca gatcatggtg gccaataccc accatgtggc catgattctc aatctgatcc
     4681 cgcgggatca atgcgacggc acggagaact ttcctcagga gggctcgccc tactgcgcga
     4741 cgctgcggcc ttttctgtca cgctttggcg cgccgtgcac caagccgccg tgggggacct
     4801 tgtcagccgt gaacctgtcc acgggcgaca tcgagtggca
//
LOCUS       DBNHQR010000469         3840 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1754020, whole genome shotgun sequence.
ACCESSION   DBNHQR010000469 DBNHQR010000000
VERSION     DBNHQR010000469.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1754020"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="AC16VR_10610"
     CDS             <1..501
                     /locus_tag="AC16VR_10610"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6011653.1"
                     /translation="VIAAISQLSLVNLNDLLGVSILPVAGWGSVWHMLVPSMVLGLGT
                     MAYLTRLMRSSMLEIVNADYVRTAKSKGLPPTRIFTRHQLRNAILPVITVLGPSIAAI
                     TTGGFVVELVFAIPGLGRYFVQAVQQLDYTVIMGTTVFYGSFLVFMVIVVDLLYGLID
                     PRVRLE"
     gene            505..1872
                     /locus_tag="AC16VR_10615"
     CDS             505..1872
                     /locus_tag="AC16VR_10615"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6011654.1"
                     /translation="MAETALTARDFEPVHAERRIQTISRPSLSYWQDAWIRLKANRRA
                     LFSLYIVLGLLAFTIAGPFVWTVDPAAQDVDQISQPPGTDRRAAVVEPYQPWDGSSVL
                     PLTEGLRLAEPATNQAVRLMWDATPGAAGYVIYRNLFPVGPENALGLPLGENFSAEDT
                     KFEDLLDLRPRRYYYSVVSLGAAGNELAQETLMVNVDRVITVAEAEARGLAEGGELAV
                     GDVVELPLHPLGTDYLGRDLLARLMEGARVSLFIGICAPFLYVLFGVAYGSAAGFLGG
                     HFDQALMRFADFVIALPFLLFMILFKIAFGIGPGESGIMPMLAAMVLLSWPGTSRLVR
                     GQILQIREEGYISASRLLGAKTGYLVLRHMIPNTMGVILVTLTFAVPSAIFTEAFLSF
                     IGMGVAPPTPSWGSMCNEGIRTMLTTPHELIFPALFISVTVLAFNLLGDGLRDALDAR
                     MRSTE"
     gene            1869..2894
                     /locus_tag="AC16VR_10620"
     CDS             1869..2894
                     /locus_tag="AC16VR_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020289227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6011655.1"
                     /translation="MSTPRLLEVEDLKVEFDTYGGVVQAVRGVSFGVDEGETLAIVGE
                     SGCGKSVTVQSIMGLTPMPPGRITSGSARLRGTDIIGRKEIGGEDIRGGQIGMIFQDP
                     MTSLNPTMNIGSQIAETLEVHRGYSKKQAFQRAVELLDMSRIPEAASRARQYPFEFSG
                     GMLQRAMIAMAIACEPAILIADEPTTALDVTIQAQILDLMLDLQRETGMAIILITHDL
                     GVVARMADEVAVMYAGQIAEQGTVDDVFYRSSHPYTLGLRAAMPNNDPARQHELKPID
                     GSPPDLFHPPKGCAYAARCPYAMRICESEDPHAYQMTERHFARCWQHHEGAAARVEGL
                     YYREARA"
     gene            2891..>3840
                     /locus_tag="AC16VR_10625"
     CDS             2891..>3840
                     /locus_tag="AC16VR_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010428636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6011656.1"
                     /translation="MSERPVLLETDHLTKHFLIGRGQEVHAVDGVSLAIHEQEIVGLV
                     GESGSGKSTYGKTVVGLHDKTSGTVTYRGERLPQRYGAADFQKHARHMQMIFQDPYSS
                     LNPRMTVGEIIGEGLRLHANLDVREVRERVAEWLKRVGLEPDHMSRYPHEFSGGQRQR
                     IGVARALILEPEFVVCDEPISALDVSVQAQVINLLDDLKRSMGLTMLFIAHDLSMVRY
                     VSDRMAVMYLGSVVEIGPANDVFFNPKHPYTEILVGSNPEPDPEAERHRASTAIVGEI
                     PSPVNVPEGCRFANRCPKVMDVCRSVTPELIPLKNAEAVR"
BASE COUNT          614 a         1371 c         1226 g          629 t
ORIGIN      
        1 gtcatcgccg ccatcagcca gctgagcctg gtcaacctca acgacctgct gggcgtctcg
       61 atactgcccg tggccggctg gggcagcgtg tggcacatgc tggtgccgtc catggtcctc
      121 gggctcggca ccatggctta cctgacgcgg ctcatgcgct cctccatgct ggaaatcgtc
      181 aacgccgact acgtccgcac ggccaagtcc aaggggctgc cgcccacgcg catcttcacc
      241 aggcaccagc tgcgcaacgc gatcctgccc gtgatcaccg tgctgggccc gtccatcgcc
      301 gccatcacca cggggggctt cgtggtggag ctcgtcttcg ccattcccgg tctcggccgc
      361 tacttcgtgc aggccgttca gcagctcgac tacacggtga tcatgggcac cacggtattc
      421 tacggctcgt tcctggtctt catggtcatc gtggtggacc tgctctacgg gctcatcgac
      481 ccgcgcgtga ggctcgagta gcccatggcc gagactgccc tcaccgcacg agacttcgag
      541 cccgttcacg ccgagcggcg gatccagacc atctcccggc cgtccctgtc ctactggcag
      601 gacgcctgga tccgcctgaa ggccaaccgc cgcgcgctgt tctcgctcta catcgtgctg
      661 gggctgctgg ccttcaccat cgccggcccg ttcgtgtgga ccgtggaccc ggcggcccag
      721 gacgtggacc agatcagcca gccaccgggc acggaccggc gtgccgccgt ggtggagccc
      781 taccagccct gggacggctc atcggtgctg ccgctcacgg aaggtcttcg actcgccgag
      841 ccggccacca accaggccgt gcggctgatg tgggacgcaa cgcccggtgc tgccggctac
      901 gtcatctacc ggaacctctt ccccgtcggc ccggagaatg ccctggggct gccgctgggc
      961 gaaaacttct ccgccgaaga cacgaagttc gaggatctgc tggatcttcg cccgcgccgc
     1021 tactactaca gcgtcgtcag cctgggcgcc gccggcaacg agctggctca ggagacgctg
     1081 atggtcaacg tcgaccgggt catcaccgta gcggaagccg aggcacgggg cctggctgaa
     1141 ggcggcgagc tcgccgtggg cgacgtcgtc gagctgcccc tgcacccgct gggcacggac
     1201 tatctgggcc gcgacctcct ggcccggctc atggagggcg cgagggtgtc cctcttcatc
     1261 ggcatctgcg cgccgttcct ctacgtgctg ttcggggtgg cctacggcag cgcggcgggc
     1321 tttctcggcg ggcacttcga tcaggcgctc atgcgcttcg ccgacttcgt catcgccctg
     1381 cccttcctgc tgttcatgat cctgttcaag atcgccttcg gcatcggccc gggcgagagc
     1441 ggcatcatgc ccatgctggc ggccatggtc ctgctctcct ggccgggcac gtcgcgcctg
     1501 gtgcgcgggc agatcctgca gatccgggag gagggctaca tcagcgcctc gcgcctgctg
     1561 ggcgcgaaga ccggctacct ggttctgcgc cacatgattc ccaacaccat gggcgtcatc
     1621 ctggtcacgc tgaccttcgc ggtgcccagc gccatcttca cggaggcgtt cctgtccttc
     1681 atcggcatgg gcgtcgcccc cccgacgccg tcctggggca gcatgtgcaa cgagggcatc
     1741 cgcaccatgc tgaccacgcc ccacgagctg atcttccccg cgctcttcat cagcgtgacg
     1801 gtgctggcct tcaacctgct cggagacggc ctgcgcgatg cccttgacgc ccgcatgagg
     1861 tccacggagt gagcacgccg cggcttctgg aggtggagga tctgaaggtg gagttcgaca
     1921 cctacggcgg cgtggtgcag gccgtccgcg gggtgagctt cggggtggac gagggcgaga
     1981 cgctcgccat cgtcggcgag tccggctgcg gcaagtcggt gacggtgcag agcatcatgg
     2041 gcctgacccc catgccgccc ggccgcatca cctcgggcag cgcccggctg cgcggcacgg
     2101 acatcatcgg gcgcaaggag atcggcggcg aggacatccg cggcggccag atcggcatga
     2161 tcttccagga ccccatgacg tccctgaacc ccaccatgaa catcggctcc cagattgcgg
     2221 agaccctgga ggttcaccgg ggctactcca agaaacaggc attccagcgg gccgtggagc
     2281 tcctggacat gagccggatc cccgaggcgg ccagccgggc gcgccagtac ccgttcgagt
     2341 tctccggcgg catgctgcag cgggcgatga tcgccatggc catcgcctgc gagccggcca
     2401 tcctcattgc cgatgagccc accaccgccc tggacgtgac catccaggcg cagattctcg
     2461 atctcatgct cgacctgcag cgcgagaccg gcatggccat catcctcatc acccacgatc
     2521 tgggggtggt ggcgcggatg gcggacgagg tggcggtgat gtacgcgggc cagatcgccg
     2581 agcaaggcac cgtggacgac gtcttctacc gctcctccca cccctacacc ctggggctcc
     2641 gggcagccat gcccaacaac gacccggccc gccagcacga gctgaagccc atcgacggca
     2701 gcccgcccga cctcttccac ccgccaaagg gatgcgccta cgccgcgcgc tgcccctacg
     2761 ccatgcgcat ctgcgaaagc gaagatccgc acgcctacca gatgacggag cgccacttcg
     2821 cccgctgctg gcagcaccac gaaggcgccg ccgcgcgcgt cgagggcctg tactaccggg
     2881 aggcgcgggc gtgagcgaac ggccggtgct tctcgagacg gaccatctca ccaagcactt
     2941 cctgatcggc cggggccagg aggtgcacgc cgtggacggc gtgagcctgg ccattcacga
     3001 gcaggagatc gtcgggctcg tcggcgagag cggatccggc aagtccacct acgggaagac
     3061 cgtggtgggg ctccacgaca agaccagcgg caccgtcacc taccggggcg aacggctacc
     3121 ccagcgctac ggcgctgccg atttccagaa gcacgcccgc cacatgcaga tgatcttcca
     3181 ggacccctac tcgtcgctga acccgcgcat gacggtgggc gagatcatcg gcgagggctt
     3241 gcggctccac gccaacctgg acgttcgcga agtgcgcgag cgggtggccg agtggctgaa
     3301 gcgcgtcggg ctcgagcccg accacatgtc ccgctacccc cacgagttct ccggcggcca
     3361 gcgccagcgc atcggcgtcg cccgggcact gatcctggaa cccgagttcg tcgtctgcga
     3421 cgagcccatc tccgcgctcg acgtctccgt gcaggcgcag gtcatcaacc tgctcgacga
     3481 cctgaaacgc tccatggggc tcaccatgct cttcatcgcc cacgatctgt ccatggtgcg
     3541 ctacgtgagc gaccgcatgg cggtgatgta cctgggctcc gtggtggaga tcggccccgc
     3601 caacgacgtt ttcttcaacc cgaagcatcc gtacacggag atcctcgttg gctccaaccc
     3661 ggagccggac ccggaggccg aacgccatcg ggcgtcaacg gccatcgtcg gcgagatccc
     3721 ttcaccggtg aacgtccccg aggggtgccg cttcgccaac cgctgcccga aagtgatgga
     3781 cgtgtgccga agcgtcacgc cggagctgat cccgttaaag aacgcggaag ccgtccgtga
//
LOCUS       DBNHQR010000470         3221 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_740966, whole genome shotgun sequence.
ACCESSION   DBNHQR010000470 DBNHQR010000000
VERSION     DBNHQR010000470.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_740966"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="AC16VR_10630"
     CDS             complement(<1..297)
                     /locus_tag="AC16VR_10630"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043023 - ribosomal large subunit binding
                     [Evidence IEA];
                     GO_function: GO:0048039 - ubiquinone binding [Evidence
                     IEA];
                     GO_process: GO:0045333 - cellular respiration [Evidence
                     IEA];
                     GO_process: GO:0045947 - negative regulation of
                     translational initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HIR6011657.1"
                     /translation="MPRVERSIEIAAPVSRVQQVIVDFERYPEFVPFMERAELLGIEG
                     ERRRVRFTLNLVARISYTLDLVPGTDSGLTWTLVDGPFVRNNGSWRLKATNDDGT"
     gene            complement(305..2323)
                     /locus_tag="AC16VR_10635"
     CDS             complement(305..2323)
                     /locus_tag="AC16VR_10635"
                     /inference="COORDINATES: protein motif:HMM:NF023649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HIR6011658.1"
                     /translation="MSGNDFVPKQAAAGLLLLAVVGCGSEEAPPADAGREQALPAGTN
                     SGHLVAYSEVREPCANADPLRIPLFGDVHVHTSFSFDAAANSVGTTPADAHKFALGEP
                     IPFFPVDASGTPTGTIRIDRPLDFLAVTDHGEFLGERSLCRDPRSPKYSSAFCEEYRT
                     GERHGMRMLGRVITSETPRRVPELCGDDGALCLEYAEGPWRRIVEAAEAAYDRSPGCT
                     FTSFVGYEYTGTPGTSNYHRNVIFRNASVPPRPVSYIDAPVDSLLWNALDAACGAVDG
                     CDYLTIPHNSNLANGRMGPYRGIELTEANRRAYAEQRQRREPVMEMFQHKGASECING
                     LTTVLGEPDELCDVEAVRVIGRAETYQTARMVDGELVLGETTEVTQECAPGESSSNGM
                     LGAGCVSATDYMRSGLLVGLAEEQEIGVNPIKLGMIAATDTHTSTPGAVAEGDWRGHV
                     TGEATPAERLEPGLLTSGIDGNPGGLAGVWAVENSRDAIFDAMLRREVFGTSGPRIVP
                     RFFAGWGYDAEMCGSADLVARGYGGGVPMGGDLPRGAPGDRPAFIAWAARDPAPSAQP
                     LESLQLVKGWIDAEGQARNRVIPIAGGEGQVARDTLCAVYRDDDFDPAVPAYYYLRVV
                     EPPTPRWSTFDCQRLPESDRPAVCSDGSYPQTIREMAWTSPIWYRPEG"
     gene            2447..>3221
                     /locus_tag="AC16VR_10640"
     CDS             2447..>3221
                     /locus_tag="AC16VR_10640"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6011659.1"
                     /translation="MPDNDDRISEPAADGELDVAIVGAGFSGLYMLHRVRDVLKLKTR
                     VFEAADGVGGTWHWNRYPGARCDSESYFYCYSFSKDLEQEWTWTSKFPEQPEILSYLN
                     HVADRFDLRRDIQLNTRVTGALFEDRSNRWIISTDTGETVSARFLISAVGCLSTRNLP
                     DIPGIESFAGEHYHTGNWPHEGVDFRGKRVGLIGTGSTGIQAAPVIAEEAEHLYVFQR
                     TPNFVVEAQNRPLPRAEMDAIKANYDALRAEAKSVFSAYV"
BASE COUNT          562 a         1098 c         1043 g          518 t
ORIGIN      
        1 ggtcccgtcg tcgttcgtgg ccttaagacg ccagctgccg ttgttgcgca cgaagggccc
       61 gtctaccagg gtccatgtca ggcccgaatc ggtgccgggc acgaggtcca gggtatagct
      121 gatccgcgct accagattca gcgtgaagcg aacgcggcgt cgttcgccct cgatacccag
      181 cagctccgcg cgttccatga agggaacgaa ctccggatag cgttcgaagt cgacgatgac
      241 ctgctgcacg cgcgatacag gcgccgcgat ctcgatgctg cgctcgaccc tgggcatctg
      301 ccgctcagcc ctcgggccgg taccagatgg gagacgtcca ggccatttcc cggatcgtct
      361 ggggatacga gccgtcgctg cacacggccg gccggtcgga ttcaggcaga cgctgacagt
      421 cgaaggtgct ccaacgcggc gtcggcggct ccaccacgcg caggtagtaa tacgcgggca
      481 cagcgggatc gaagtcgtca tcgcgataaa cggcgcaaag ggtgtcccgc gctacctgcc
      541 cctctccgcc agcaatggga atgacccggt tgcgcgcctg accctcggcg tcgatccatc
      601 ctttgaccag ctgcagcgac tcgagcggct gggccgaggg cgccggatcc cgcgccgccc
      661 aggcgatgaa agcgggccgg tcacccgggg caccccgggg gagatcgccg cccatgggca
      721 ccccgccccc atagcctcga gccaccaggt cggcgctgcc gcacatctcg gcgtcgtagc
      781 cccagcccgc aaaaaagcgc ggcacgatgc ggggcccgga ggtgccgaac acctcccgcc
      841 ggagcatggc gtcgaatatg gcatcgcgcg aattttccac ggcccagacg cctgccaggc
      901 cgccaggatt gccgtcgatc ccgctggtca gcagccccgg ctcgaggcgc tcggccggcg
      961 tcgcttcgcc ggtcacgtgg ccgcgccagt cgccttccgc cacggccccg ggagtggagg
     1021 tgtgggtgtc cgtggcggcg atcatgccga gctttatggg attgaccccg atttcctgct
     1081 cctcggccag gcctaccagg agcccggagc gcatgtaatc cgtggcagac acgcagccgg
     1141 cgcccagcat gccgttgctg gaagactccc cgggcgcgca ttcctgggtg acctcggtgg
     1201 tctcccccag caccagctca ccgtcgacca tccgcgccgt ctggtacgtc tccgcgcggc
     1261 cgatcacccg gacggcctcc acatcgcaca gctcgtccgg ctcgccgagc acggtggtca
     1321 gcccgttgat gcactccgat gcccccttgt gctggaacat ctccatgacg ggctcccggc
     1381 gctgacgctg ctccgcgtaa gcacgccgat tcgcctcggt gagctcgatg ccccgataag
     1441 ggcccatccg tccgttggcc aggttcgagt tgtgcggaat ggtcagatag tcacaaccat
     1501 cgacggcacc gcaggccgca tccagcgcgt tccagagcag actgtccacg ggcgcatcga
     1561 tgtaagacac cggccgcggc gggacgctgg cattgcggaa gattacgttg cggtggtagt
     1621 tcgaggtgcc aggcgtgccc gtgtactcgt agccgacgaa gctggtgaac gtgcagccgg
     1681 gcgagcgatc gtaggcggcc tcagcggcct cgacgatccg gcgccacgga ccctcagcgt
     1741 attccagaca cagcgcgccg tcgtctccac acagctcggg cacccgtcgc ggcgtctccg
     1801 aggtgattac cctgcccagc atgcgcatgc cgtgtcgctc gccagtccgg tactcctcgc
     1861 agaaagcgga cgagtacttc ggcgagcgcg ggtcccggca cagagaccgc tcgcccaaga
     1921 actccccgtg gtcggtcacc gcgaggaaat ccagcggccg gtcgatgcgg atggtgcccg
     1981 tcggcgtgcc gctcgcgtct accggaaaga agggtatggg ctcgccaagc gcgaacttgt
     2041 gcgcatccgc gggcgtggta cctacgctgt tcgccgcggc gtcgaacgag aagctggtgt
     2101 gaacgtgaac gtcgccgaac aggggtatgc gcagcggatc ggcgttggcg caaggctcgc
     2161 gcacttcgct gtaggcaacg agatgccccg agttcgtgcc ggcaggcaac gcctgttccc
     2221 gcccggcgtc cgccgggggc gcctcttcac ttccgcagcc gacgacggcg agcagcagca
     2281 agccggctgc cgcctgcttg ggaacgaaat cgtttccgct cacgtcaaat ccctcccgat
     2341 cctcgcgacc gtcaacctta gcatggagcc gatcggccgc gccggtcacg acttgcaggt
     2401 cacggcgaaa ccggcaaact agaaagaagg tcggagggag agaagcatgc cagacaacga
     2461 cgacaggatt tccgagccag cggcggacgg cgagctcgac gttgccatcg tcggcgcggg
     2521 cttttccggc ctctacatgc tgcaccgggt gcgcgacgtg ctgaagctga agacgcgcgt
     2581 cttcgaggcg gcggacgggg tcggcggcac gtggcactgg aaccggtacc caggtgcccg
     2641 ctgcgattcg gaaagctact tctactgcta ctcgttctcc aaggatctcg agcaggagtg
     2701 gacgtggacg agcaagttcc ccgagcaacc ggaaattctc agctacctca atcacgtggc
     2761 agaccggttc gatctgcgcc gtgacattca gctcaacaca cgagtcaccg gcgccctttt
     2821 cgaagatcgg agcaaccgct ggataatctc caccgacacc ggcgagaccg tgtccgcccg
     2881 ctttctgatc agcgcggtgg gttgcctctc gacccggaac ctgccggata ttcccggcat
     2941 cgagagcttt gccggcgagc actaccacac gggcaactgg cctcatgaag gcgtcgactt
     3001 ccggggaaag cgcgtgggcc tcatcggcac gggctccacc ggaatccagg cggcaccggt
     3061 gatcgccgag gaagccgagc acctctacgt ctttcagcgc acgccgaatt tcgtcgtcga
     3121 ggcgcagaac cggccgctgc cgcgcgctga gatggacgcg atcaaggcga attacgatgc
     3181 gctgcgcgcg gaggcgaaat cggtcttcag cgcctatgtg t
//
LOCUS       DBNHQR010000471         4109 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_995263, whole genome shotgun sequence.
ACCESSION   DBNHQR010000471 DBNHQR010000000
VERSION     DBNHQR010000471.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4109)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4109)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4109
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_995263"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="AC16VR_10645"
     CDS             complement(<1..377)
                     /locus_tag="AC16VR_10645"
                     /inference="COORDINATES: protein motif:HMM:NF013053.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HIR6011660.1"
                     /translation="MPALDKTSVGLGERPALLVVDASVAFTDPACALGADFSAEIGVI
                     AQLMALAGESGWPRFFSTVWYESDDEARVFREKVPALNLLDAGSPLVAIDPRLPVDAS
                     DRVFRKTHASCFFGTDLYRWLREA"
     gene            complement(384..2267)
                     /locus_tag="AC16VR_10650"
     CDS             complement(384..2267)
                     /locus_tag="AC16VR_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018403849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HIR6011661.1"
                     /translation="MTGRILQTNDAPFTRLDVDPVTLDIIENALRNARVEMDATLFRT
                     AMSPGIREQGDAFPMIANVEGKMVVGQFGSFIHGFMEAFDGVLEEGDMIFTNDPYSCG
                     AAVSHLNDWLVLKPIFKDGRHLCWAAHFGHMTDIGGKVPGSLPIDAREIFEEGVAVPP
                     VKIFRKGELQEDIISIVLRNSRMPHWNKGDFNGVIAACNMAERRCIEIAERFGDDVFY
                     SAMDELLARNCRAMTRLIQDTVPETKQVFEDYICDDGMGMGPYKIRCAMWREGDKVIF
                     DFDGTDPQSSGSINFLLNEEMFKMFCGTFMISVFDPQILFNDGFYDFMEVRIPEGCML
                     KPRRPAALSCRTHALGRIFDVISGLLGQGNPEFLCAAGFSDSPHLMYSGYDKEGEWYQ
                     LFQIGFGGIPGRPMGDGPDGHSLWPGFTNVPNEYIEAYFPLRIETYETIPDSGGPGQN
                     RGGNGIRMGYRFLEDGQISIHDDRWLTYPWGVNGGLPGGRGSKVLVRVDGTQEQLPSK
                     CDRIEVAEGDILYYNTWGGGGAGDPFEREPERVGKDVERGLVTAEGARRYGVVCDAAG
                     VVDADATEQLRSQLRAARQEIPLFDFGGTVDEIKRRCREETGLEPPDAPVFARRGSGL
                     AAG"
     gene            complement(2279..>4109)
                     /locus_tag="AC16VR_10655"
     CDS             complement(2279..>4109)
                     /locus_tag="AC16VR_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008937730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HIR6011662.1"
                     /translation="TTRGYRQVLQIARSYVPGGLGGWVIFNKSDPMAPLECTVEVPER
                     IGADGSVVEALDEDAARSALRSLGARGIEALTVSLINSFTNPAHEERLAEIAAEELPG
                     VPVSLSSEVIPEMQEYERTITTVANSYVRPEVSRYIDNLQRELDERMNSPKLSILRSD
                     GGLSTAPAAAKYPVNLLMSGPAGGVAGALWVGRQAGFDNLLTFDMGGTSTDVSLIEQG
                     KAVVRRETTVGDVNVRASSMDVRTVGAGGGSIAYVPELTGALRVGPDSAGAVPGPAAY
                     DLGGERPTVTDANVVLGYLPAEQKLGGDMTISRDKAAAAVQQIADAMGLSLEAAAEGI
                     VKIVNENMFGALRLVSVEKGYDPRNFSLIAFGGAGPLHANALGKLTQAWPVIVPPGPG
                     VLCAYGDATTRLRDEASRTLISKLDDIEPAAIADELKQLEALARQAMLAEGVGDAAAQ
                     VTDADITAQFQVDVRYSGQGLVLTVDTSEEDLLSDGFAKVSEAFDRLHDQLFTFALDA
                     DKEFVNLRVVVEGPEAHVSASELEPGAADPAAASLGEHQIYADGQTHAGTLYDRARLH
                     PGNVVPGPAVIMEMDSTTLILPGHHAEVDRIGNLLIRPDGHDD"
BASE COUNT          702 a         1385 c         1309 g          713 t
ORIGIN      
        1 gcctcccgaa gccagcggta cagatcggtg ccgaagaagc aggatgcgtg ggttttgcgg
       61 aacaccctgt cggaggcatc cacgggcaac ctcgggtcga tggcgaccag ggggctgccc
      121 gcatcgagca gattcagagc ggggaccttt tcccggaaca cgcgggcttc gtcatcggac
      181 tcgtaccaga cggtggagaa aaaccggggc cagcccgatt cgccggccag cgccatcagc
      241 tgcgcgatga cgccaatctc agccgagaag tcggcgccga gcgcgcacgc ggggtcagtg
      301 aaagccacgc tcgcatccac cacgagaagg gccggacgct cgcccagacc gaccgacgtc
      361 ttgtccaggg caggcacgac ctgctacccc gccgccagac cgcttccccg gcgggcaaat
      421 accggtgcat ccggcggctc cagaccggtc tcctcgcggc atcggcgctt gatctcatcc
      481 accgtgccgc caaagtcgaa caggggaatc tcctggcgcg ccgcccgcag ctgcgacctg
      541 agctgctcgg tggcgtccgc atcgacgacc cccgcagcgt cgcagaccac gccgtagcgg
      601 cgggcacctt ccgcggtgac caggccgcgc tcgacgtcct tgcccacccg ctcgggctca
      661 cgctcgaaag gatcacccgc gccgccgccg ccccaggtgt tgtagtagag gatgtctcct
      721 tcggcgacct cgatccggtc gcacttggat ggcagctgtt cctgggtgcc gtcgaccctg
      781 accagcacct tgctgccccg gccgccgggc agcccgccgt tgactcccca gggataggtg
      841 agccagcgat cgtcgtgtat ggagatctgg ccgtcttcca ggaaacggta gcccatccgg
      901 atcccgttgc cgcccctgtt ctggccgggc cctccggagt cagggatcgt ctcgtaggtc
      961 tcgatgcgca gcgggaagta ggcctcgatg tactcgttgg gaacattcgt gaagccgggc
     1021 cacagcgagt ggccgtcggg cccgtcaccc atgggccggc ccggaatgcc tccgaagccg
     1081 atctggaaga gctggtacca ctcgccctcc ttgtcgtagc ccgagtacat caggtgcggg
     1141 ctgtcggaga acccggcggc gcagaggaac tcgggattgc cctgcccgag caaacccgag
     1201 atcacgtcga agatgcgtcc gagagcgtgc gttcggcaag acagcgccgc gggtcggcgc
     1261 ggcttgagca tgcagccctc ggggatgcgc acctccatga agtcgtagaa gccgtcgttg
     1321 aagagaatct gagggtcgaa caccgagatc atgaacgtcc cgcagaacat cttgaacatc
     1381 tcttcgttga gcaggaaatt gatggagccc gagctctggg gatcggtgcc gtcgaaatcg
     1441 aagatcacct tgtccccttc gcgccacatc gcacagcgga tcttgtacgg ccccatgccc
     1501 atgccgtcgt cgcagatgta gtcctcgaaa acctgcttgg tctccggaac ggtatcctgg
     1561 atcagccgcg tcattgcccg acagttgcgg gccagcaact cgtccatggc agagtagaac
     1621 acgtcgtcgc cgaaacgctc ggcgatctcg atgcagcgcc gctccgccat gttgcaggcg
     1681 gcgatcaccc cgttgaagtc ccctttgttc cagtggggca tgcgcgagtt gcgcaggacg
     1741 atgctgatga tgtcttcctg cagctcgcct ttgcggaata tcttcaccgg cggcaccgcg
     1801 acgccttcct cgaagatctc ccgggcgtcg atgggcagag agcccggcac tttgccgcca
     1861 atgtcggtca tgtggccgaa gtgggcggcc cagcagaggt gccggccgtc tttgaagatc
     1921 ggcttcagta ccagccagtc gttgagatgg ctcaccgcgg cgccgcagct gtacgggtcg
     1981 ttggtgaata tcatgtcccc ttcttcgagc accccgtcga aggcttccat gaacccgtgg
     2041 atgaacgagc cgaactggcc caccaccatc ttcccttcga cgttggcgat catggggaac
     2101 gcatccccct gctcccggat gcccggcgac atggccgtcc ggaacagcgt ggcgtccatt
     2161 tccacccgag cattgcgaag cgcgttctcg atgatgtcca gggtcaccgg gtccacgtcc
     2221 agccgcgtga acggagcgtc gttggtctgt agtattcgtc ctgtcatgtg agcgcccctc
     2281 agtcgtcgtg cccgtccggg cggatgagca gattgcctat acggtcgacc tcggcgtgat
     2341 gccccggaag gataagagtg gtcgagtcca tctccatgat caccgccggc cccggcacca
     2401 cgttacccgg gtggagtcgc gcccggtcgt aaagcgttcc cgcgtgggtc tgcccgtcgg
     2461 catatatctg gtgctccccc agggaggcgg ccgcgggatc ggccgcgccc ggctccaact
     2521 cggacgcgga aacgtgcgcc tccggccctt ccaccaccac acgcagattg acgaactcct
     2581 tgtccgcatc cagcgcaaag gtgaagagct ggtcgtggag ccggtcgaac gcctccgaca
     2641 ccttcgcgaa cccatctgag agcagatcct cttcgctggt atccacggtg agcacgaggc
     2701 cctggccgct gtagcgaacg tcgacctgga actgcgccgt gatgtcggca tcggtgacct
     2761 gagccgcagc gtccccgacg ccttcggcca gcatggcctg ccgggcgagc gcttcgagct
     2821 gcttgagctc gtcggcaatg gccgccggct cgatgtcgtc cagcttggag atcagcgtcc
     2881 gggaagcttc gtcgcgcagc ctggtggtcg cgtcgccata ggcacacagc acgccgggcc
     2941 ctggcgggac gatcacgggc caggcctggg tcagcttgcc cagcgcgttg gcgtgcagcg
     3001 gtccggcacc gccaaaggcg atgagggaaa agttccgggg atcgtaaccc ttctccaccg
     3061 agaccagccg cagggcgcca aacatgttct cgttgacgat cttgacgatg ccttcagccg
     3121 cggcttcgag ggagagcccc atggcgtcag cgatctgctg cacggcggcc gccgctttgt
     3181 cccggctgat ggtcatgtcg ccgccgagct tctgctccgc gggcaggtag ccgagaacca
     3241 cgttggcatc ggtgaccgtc gggcgctcgc cgcccagatc ataggcggcc ggcccgggaa
     3301 ccgctccggc cgaatccgga cctacgcgca gcgcgccggt cagctccggc acgtaggcga
     3361 tggacccgcc gccggcaccc actgtgcgca cgtccatgga gcttgcccgg acgttgacgt
     3421 caccaacggt ggtttccctg cgcaccacgg ctttgccctg ctcgatgagg gacacatccg
     3481 tggacgtgcc gcccatgtcg aaggtcagga ggttgtcgaa acccgcctgc cggccgaccc
     3541 aaagagcgcc ggcaacccca cctgccggcc cgctcatgag caggttcacg gggtatttgg
     3601 ccgcagccgg agccgtggag aggccaccgt ccgatctcag aatcgatagc ttcggcgagt
     3661 tcatgcgctc gtcgagttcc cgctgcagat tgtcgatgta acgcgacact tcgggcctga
     3721 cgtaggagtt agccacggtc gtgatggtgc gttcgtactc ctgcatctcc ggaatgactt
     3781 ccgatgacag agaaacaggc acgccgggta gctcttccgc ggcgatctcc gctaggcgct
     3841 cctcgtgggc cgggttggta aagctgttga tgagcgatac ggtcaatgcc tcgatgccgc
     3901 gggcgcccag ggaacgaagc gccgatctgg cggcgtcttc gtcgagcgct tcgactaccg
     3961 agccgtccgc gccgatccgc tcggggacct ccaccgtgca ctccagcggc gccatgggat
     4021 ccgacttgtt gaagatcacc cagccgccca agccgccggg cacgtaggat cgggcaatct
     4081 gcaggacctg ccggtacccg cgcgtggtc
//
LOCUS       DBNHQR010000472         7817 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_298822, whole genome shotgun sequence.
ACCESSION   DBNHQR010000472 DBNHQR010000000
VERSION     DBNHQR010000472.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7817)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7817)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7817
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_298822"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..560
                     /locus_tag="AC16VR_10660"
     CDS             9..560
                     /locus_tag="AC16VR_10660"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019410888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HIR6011663.1"
                     /translation="MDGPVGRLLLAGDGKRLKLIGFSTGARARGAQQDWERYDEPFRP
                     AKRQLDEYFDGRRRYFDIELAPDVTPFQGKVLEALLEIPYGETRSYGEIARAIGQPRA
                     VRAVGGANGSNPIPIVIPCHRVIGSNGSLTGFGGGLDTKQYLLDLERSHSGLFEGLVG
                     DSQQAPQRPVRAGKGQVVFTVTR"
     gene            complement(570..1115)
                     /locus_tag="AC16VR_10665"
     CDS             complement(570..1115)
                     /locus_tag="AC16VR_10665"
                     /inference="COORDINATES: protein motif:HMM:NF016269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HIR6011664.1"
                     /translation="MRSFLRFLTLCAVCLAPAGAAWSASKVEIDARISAALESLYEIE
                     PAARELAAKASGVLVMPRVYKLGFGLGGEYGEGALLINNQAVQYYRVTAASFGFQLGG
                     QAKSEVLLFMSSEALDGFRNSDGWEAGVDGAITFIEFGVGKDFDTHTLQDPIIGFVYG
                     NKGLMYDLSLEGSKFWRIDKN"
     gene            1346..1930
                     /locus_tag="AC16VR_10670"
     CDS             1346..1930
                     /locus_tag="AC16VR_10670"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HIR6011665.1"
                     /translation="MDTNGLRRDPEGARRRILDSAAEHFSRHGPDGARVDAIAAAARV
                     NKRMLYHYFGDKQALFRAVLDDRAARPGGTGNTSGWDTTTARLLVWAAGLDPVGATPG
                     NLEQWTALARHLGDEQDRGRLRNDIRAGTAAMLYLALAALPALLPAHVRAILGSNDAA
                     MLQGDLRKLLNPPAELHGGRGRPRVRMQPQVRPR"
     gene            complement(1933..3210)
                     /gene="aroA"
                     /locus_tag="AC16VR_10675"
     CDS             complement(1933..3210)
                     /gene="aroA"
                     /locus_tag="AC16VR_10675"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIR6011666.1"
                     /translation="MGSLTIQPIQALEGDITLPGSKSISNRALLLAAEAQGQTRLVNL
                     LDSDDTAYMLRALGQLGISITENGGDHLVTGVAGPLVNDERHESLDLGLAGTALRPLT
                     AALTLGRGVFELDGTARMRERPVGHLVEGLRQLGASIRYTGAEGFPPIEVTGTGLLGG
                     RVTMQGNVSSQFLTSLLLAAPLAAGPVEVVVEGEQVSKPYLDITVDMMRRFGATVGHE
                     DHRLFRVEPGGYQSPGRYLIEGDASAASYFLAAGAIRGRGVRVHGIGAGSVQGDMAFV
                     EVLKAMGARVTVDAEWVRVEPGALTGVDLDLNHIPDAAMTLAVLALFAEGSTRIRNIY
                     NWRVKETDRLAAMATELKKLGAEVDECEDSLTVNPPKYMNAASIDTYDDHRMAMCFSL
                     AALGGVAVTINDPDCVSKTFPRYFAEFERLARR"
     gene            complement(3257..3847)
                     /locus_tag="AC16VR_10680"
     CDS             complement(3257..3847)
                     /locus_tag="AC16VR_10680"
                     /inference="COORDINATES: protein motif:HMM:NF018996.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein Syd"
                     /protein_id="HIR6011667.1"
                     /translation="MSVQTALSQFVSHYEKSYPELTEIFDPEWRSPCETSEPYQDDDG
                     IVRVPWSPLARGLKVPEEHDFAGLENALETEIHPDIKAYYGSFWSGGLEAEAPQGHVS
                     LILLWNAEDASRLIENLIGHALAKRRARAPLSVFFACTDQDSELFLSVENTTGEVLIE
                     KPGHKPVDVVAGSLASFIDSLVPAPPELHPERSLLR"
     gene            complement(3886..4374)
                     /locus_tag="AC16VR_10685"
     CDS             complement(3886..4374)
                     /locus_tag="AC16VR_10685"
                     /inference="COORDINATES: protein motif:HMM:NF025173.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HIR6011668.1"
                     /translation="MPRSKWLVLLLVLSLGANLAMAGFLAGRATGDGGGPRLLDPNLR
                     VTRMLLQQLPEERREALRPLVRDQVRSIRPSVGGIREAQRDIEAALVAEPFHPEKLEE
                     ALGAFRTHLSRSQETSHAAFVGLARSLSPEERRLLGKALAGSGRHRPHGHPPPGMPAV
                     RP"
     gene            complement(4364..4780)
                     /locus_tag="AC16VR_10690"
     CDS             complement(4364..4780)
                     /locus_tag="AC16VR_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011669.1"
                     /translation="MNIDDFEQGLDRWGSDLDSWPDPERDAASMLVERSERARALMVQ
                     GQALDGWLDEGRTHRAPMQLKQKILDQLPRPDIWQRAADWFASAFWPPALAGACALLL
                     GFATGTLLPAANEEALIDDVAGLAFTETYLEFEDAP"
     gene            complement(4806..5315)
                     /locus_tag="AC16VR_10695"
     CDS             complement(4806..5315)
                     /locus_tag="AC16VR_10695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR6011670.1"
                     /translation="MVLIAKQDHEAFSHLLTRHLDSIHRYLVRLAGSTADADELAQET
                     FLRVWLNARTFEAGRVAVTTWMHRIAHNVCVDELRRRRRTTSLDEAPEPGVDERGVHQ
                     ASERLEHLQAALGSLPEAQRSAIVLSRIQGFSNQDAARIMNVSVHALESLLARARRNL
                     RLRLADLSE"
     gene            complement(5359..5961)
                     /locus_tag="AC16VR_10700"
     CDS             complement(5359..5961)
                     /locus_tag="AC16VR_10700"
                     /inference="COORDINATES: protein motif:HMM:NF024890.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HIR6011671.1"
                     /translation="MTMKTTMTLALLALTGLATGTSWSHHHDRDFPISIAEAEARAAE
                     AFGEIDANGDGKITSEEFANAERPMPKGRHHAHSGTHRAERGAHHRPGHHGDGPPPDQ
                     AALDAALFDALDADGDGKLSRGEFSHEKMHENHQRLRREETFARLDSNDDGVLTNDEL
                     LDRRLERLKKVDADGDGNVTRDEMKAAMREHHGGDRGPGG"
     gene            complement(6015..6518)
                     /gene="greB"
                     /locus_tag="AC16VR_10705"
     CDS             complement(6015..6518)
                     /gene="greB"
                     /locus_tag="AC16VR_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HIR6011672.1"
                     /translation="MSRYRPPRPRSSPYITPAGQERLAAELKELWKVKRPEVTQKVAE
                     AAALGDRSENAEYIYGKRQLREIDARIRFLAKRLDELTVVDRPPRDQGKVFFGAWVRL
                     ADETGEEARYRLVGSDEFDRAPEFISIDSPVARLLLGKSVGDEVDLPGAGGERTWEVV
                     AIGYEPA"
     gene            complement(6567..>7817)
                     /locus_tag="AC16VR_10710"
     CDS             complement(6567..>7817)
                     /locus_tag="AC16VR_10710"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR6011673.1"
                     /translation="GYQGSAATELAAITPFEPEGSPFGTPGQWVIGMGYDDSLIEEQR
                     HPERSDLDAISSEHPIALIHVSGHLTTVNSMALDLSGITAETPDPPGGMIRRQPGSNE
                     PNGVLEETATAPLRHIYSGGYGGGGGAPGADLRKALAMYAGNGVTAVQDGGASWEIHE
                     MVREASIAGDVNLDVVLYPVGMDPTFSVPEGVTLGEYQDRVKVGGIKLLLDGSPQGKT
                     AYLSKPYEVAPHGQPDDYRGYPMIPQAMADERVAHFIGRGIPMLVHCNGDAAAEMLLD
                     ALDKANETMDLGDHRTVMIHAQTVREDQLDRMAALGVVPPDMMRLLWATVNRITRSGQ
                     TLGEAQRISALEAIRAVTVHAAYQNFEEDLKGSLTPGKQADLVILSANPVTVDPQQIL
                     DIEVVETISRGVTVYQEPSLSASL"
BASE COUNT         1264 a         2537 c         2631 g         1385 t
ORIGIN      
        1 acacctatat ggacggcccg gtgggccgac tgctgctcgc cggtgacggc aagcgcctga
       61 agctgatcgg cttctccacg ggcgcccggg cgcgcggtgc gcagcaggac tgggaacgct
      121 acgacgagcc gtttcgcccg gcgaaacgcc agctcgacga gtacttcgac ggccggcgcc
      181 gctacttcga catcgagctt gcgcccgacg tgaccccgtt tcagggcaag gtgctggagg
      241 cgctgctcga gatcccgtat ggggagacac gttcttacgg cgagattgcg cgggccatcg
      301 gtcagcctcg cgcggtgcgg gccgtcggcg gggccaacgg cagcaatccg atccctatcg
      361 tgatcccctg ccacagggtc atcggcagca acggctcgct cactggcttt ggcggtggcc
      421 tggacaccaa gcagtacctc ctggacctgg agcgcagcca ctccggactc ttcgagggtc
      481 tcgtcggtga cagccagcag gcgccccaac gacccgtgcg tgcgggtaaa gggcaggtgg
      541 tgttcacggt cacccgctag acggctcgct cagttcttgt cgatcctcca gaacttggag
      601 ccttccagcg aaagatcgta catcaggccc ttgttgccgt agacgaagcc gatgatcggg
      661 tcctgcaggg tgtgggtgtc gaaatccttg ccgacgccga actcgatgaa ggtgatggcc
      721 ccgtccacgc cggcctccca gccgtcactg ttgcggaagc cgtccagcgc ttcggatgac
      781 atgaacagca agacctcgga tttggcctgg ccgccgagct ggaaaccgaa cgacgcggcc
      841 gtcacccggt agtactgcac cgcctgattg ttgatcagca gcgcgccctc gccgtactcg
      901 ccgcccagcc cgaacccgag cttgtagacg cgcggcatca ccagcacgcc ggaggctttt
      961 gccgccagtt cccgcgcggc gggctcgatc tcgtagagcg attcaagggc ggcggagatg
     1021 cgggcgtcga tctcgacctt ggaggcagac catgcggccc cggcaggtgc aaggcagacg
     1081 gcgcaaaggg tgaggaatcg aaggaacgaa cgcatcgggc atcatcctgc tcggttgaga
     1141 ccggggcgtt ttataccacc gggcggagcc ttggtcgagg gcagaaatta tgtaaccaat
     1201 tgtttactat acctgtcgcg aagggccggc aagcgaatgg tccagatacc ggccggacgt
     1261 tcgctgacgg agcccaggcg aaagtagtaa ctaaatggat atgtatgatc ttttcagccg
     1321 ctgactggca gtattgagta accaaatgga tacaaacggc ctcaggcgtg accccgaggg
     1381 agctcgccgg cgaattctgg attcggctgc cgagcacttc tcgcgccatg gcccggacgg
     1441 tgcccgggtg gacgccatcg cggccgccgc ccgtgtcaac aagcggatgc tctatcacta
     1501 ctttggtgac aagcaggcgc ttttccgggc ggtcctggac gatcgcgcgg cgcggcctgg
     1561 cggcacaggg aacacgtccg gctgggatac cacgacggcg cgcctgctgg tctgggcagc
     1621 cggtctggat ccggtcgggg cgacgccggg aaacctggag cagtggacgg cgctggccag
     1681 gcacctgggt gatgagcagg acaggggccg gttgcgcaac gatatccggg ccggtaccgc
     1741 ggcgatgctt tatctcgcct tggccgcctt gcccgcgctg ttgccggctc acgtgcgggc
     1801 catcttaggc tccaacgatg ccgcgatgct gcagggggac cttagaaagc ttctgaatcc
     1861 gcccgccgag ctccacggcg gcagggggcg tcccagggta cgcatgcagc cccaggttcg
     1921 tccgcgctga ggtcagcggc gtgcgagacg ctcgaactcc gcgaagtagc gcggaaacgt
     1981 cttgctgacg cagtccgggt cgttgatcgt gaccgccacg ccgcccaacg cggccagcga
     2041 gaaacacatg gccatgcggt ggtcgtcgta ggtgtcgata gaggctgcgt tcatgtattt
     2101 tggaggattg actgtaagtg agtcctcaca ttcatctacc tcggcaccca gcttcttaag
     2161 ctccgtcgcc atggcggcaa ggcggtccgt ctccttgacc cgccagttgt atatgttccg
     2221 gattcgtgtg gaaccctcgg cgaagagcgc gagcacggcc agcgtcatgg cggcatcggg
     2281 gatgtggttc aggtccagat cgacgcccgt gagtgctccc ggctcgaccc gcacccattc
     2341 ggcgtctacg gtcacccgcg cccccatggc ctttaggacc tcgacgaagg ccatgtcgcc
     2401 ctggacggaa cctgctccga tgccgtgcac gcggacgccc cggccacgga tggcgcccgc
     2461 ggcgaggaaa taggaagcgg cggatgcgtc gccttcgatg aggtagcgcc cgggcgattg
     2521 gtagccgccg ggctcgacgc gaaacaggcg atgatcctcg tggccgacgg tcgcgccgaa
     2581 gcggcgcatc atgtctacgg tgatgtcgag atagggcttg gacacctgct cgccctccac
     2641 gacgacctcc accggtcccg cggccagggg ggcggcgagc agcagggagg tcaggaactg
     2701 gctggacacg ttgccctgca tggtcacgcg cccgccgaga aggccggtgc cggtcacctc
     2761 gatgggcggg aagccctccg cgccggtgta gcggatgctc gcgccgagct ggcgcagccc
     2821 ttccaccagg tgcccgacgg gccgttcgcg cattctcgcg gtgccgtcca gctcgaatac
     2881 gcctcggccc agggtcagtg ctgcggtgag tggccggaga gcggtgccgg ccaggccgag
     2941 atccagagac tcgtggcgct cgtcgttgac caggggcccg gccacgccgg tcacgagatg
     3001 gtcgccgccg ttctcggtta tggatattcc cagctgcccc agggcgcgaa gcatgtaggc
     3061 cgtgtcgtcg ctgtcgagca ggttcacaag acgggtctgg ccttgggctt cagcggccag
     3121 gagcagggcg cggttcgaaa tgctcttgga gcccggcagc gtgatgtcgc cttccagagc
     3181 ctggatgggt tgaatggtca gagatcccat ggcgtgggat taaacgacaa accgggtgcg
     3241 ggcgcgaacc tgcgcgttac cgcagcagac tgcgctcagg gtgcagctcc ggaggtgcgg
     3301 gcaccagcga gtcgatgaag ctggcgaggc ttccggccac cacgtccacc ggcttgtgac
     3361 cgggcttctc gatcagcacc tcaccggtgg tgttctccac ggagagaaag agctccgagt
     3421 cctgatcggt gcaggcgaag aagacgctga gaggcgccct ggctcgccgt ttggccagcg
     3481 cgtgcccgat gaggttctcg atcagccggg aagcatcctc tgcgttccac agaagaatga
     3541 ggcttacgtg accctggggc gcctctgcct cgagccctcc cgaccagaag ctgccgtagt
     3601 aagccttgat gtccgggtgt atttcagtct ccagggcgtt ctccaggccg gcgaagtcgt
     3661 gctcctcggg aactttcagc ccgcgggcaa gcgggctcca gggtacccgg acgatgccgt
     3721 cgtcgtcctg gtagggctcg ctcgtctcgc agggcgagcg ccattcggga tcgaatatct
     3781 ccgtaagctc ggggtaggac ttctcgtagt gggagacgaa ttgggaaagc gcggtctgga
     3841 cggacaacga agatctcgct tggaatcgag gcggtatttt acctgctacg ggcgcacggc
     3901 aggcatgccg gggggcgggt gcccgtgagg ccgatggcgg cctgagcccg ccagcgcctt
     3961 cccgagcagg cgacgctcct cgggtgagag agatcgcgcc agcccgacga aggcggcgtg
     4021 actggtttcc tggctgcggc tcagatgggt acggaacgcc ccaagcgcct cctcgagctt
     4081 ttcggggtgg aacggctcgg ccaccagcgc ggcttcgata tcccgctgag cctcacggat
     4141 cccgccgacg cttggccgga tcgacctgac ctggtcgcgc acgagcgggc gcagcgcctc
     4201 gcggcgctcc tccggaagct gttgcagaag catccgggtc accctcagat tgggatcgag
     4261 cagacgaggt ccgcccccat caccggttgc gcgtcccgcc aggaatccgg ccatggcgag
     4321 gtttgcgccc agcgacagga ccagcagaag aacgagccat ttgctacggg gcatcttcga
     4381 actccagata ggtctcggta aacgcaagcc ctgcgacgtc atcgatcagg gcttcttcgt
     4441 ttgccgccgg cagcagcgtg cccgtggcga agcccagcag cagcgcgcaa gcgccggcca
     4501 gcgccggtgg ccagaacgcg gaggcgaacc agtcggccgc gcgctgccag atgtccgggc
     4561 gcggcagctg gtcgagaatc ttctgtttga gctgcatcgg cgcgcgatga gttcggccct
     4621 cgtcgagcca gccatccaga gcctgcccct gaaccatgag cgcccgggcc cgctccgaac
     4681 gttccaccag catcgacgcg gcgtctcgtt ccggatccgg ccaggagtcg agatcggatc
     4741 cccagcggtc caggccctgt tcgaagtcgt cgatgttcaa cgcgtggctc cccttgttaa
     4801 cccgctcatt cgctgaggtc ggccaaccgt aatctgaggt tgcgccgggc tcgcgccagc
     4861 agcgactcca gggcgtggac gctgacattc attatccggg ccgcgtcctg gttcgaaaaa
     4921 ccctgaatcc ggctcagcac gatggccgag cgctgcgcct cgggcagcga gccgagcgcg
     4981 gcctgcagat gctcgagccg ctcgctggcc tggtgcacgc cacgctcgtc gaccccgggt
     5041 tcgggcgctt cgtctaggct cgtcgtccgg cggcggcgtc gcaactcgtc gacgcagacg
     5101 ttgtgggcga tgcggtgcat ccaggtggtt accgccaccc ggccggcctc gaaggtgcgg
     5161 gcattgagcc atacgcgcag gaacgtctcc tgggcgagtt cgtcggcgtc ggcggtcgag
     5221 ccggcgagac ggacgaggta gcggtgaatg gaatcgagat gacgagtcag cagatggctg
     5281 aacgcctcgt gatcctgctt ggctatcagc accatcagcg cttcgtccgt cggctctccc
     5341 accctcgagc gtgtcgggtc agccgccggg cccgcggtcg ccgccgtgat gctcgcgcat
     5401 cgcggctttc atctcgtcgc gggtgacgtt cccatcaccg tcggcgtcca ctttcttcaa
     5461 acgctcgaga cgacggtcca gcaactcatc gttcgtgagc acgccgtcgt cgttgctgtc
     5521 cagacgcgcg aacgtctctt ccctgcgcag acgctgatgg ttctcgtgca tcttctcgtg
     5581 gctgaactcg ccccgggaca gcttgccgtc gccatcggcg tcgagggcgt cgaacaacgc
     5641 ggcgtccagg gcggcctggt cgggtggcgg cccatcgccg tgatggcccg gtcgatgatg
     5701 cgctccccgt tcggcgcgat gggtaccgga gtgagcgtga tggcgccctt tcggcatcgg
     5761 ccgctccgcg ttggcgaatt cctcgctggt gatctttccg tcgccgttcg cgtcgatttc
     5821 tccgaacgcc tcggccgcgc gggcctctgc ttccgcgatg gatatcggga agtcccggtc
     5881 gtggtgatgg ctccagctcg tcccggtggc gagaccggtc agggcgagca gagcaagggt
     5941 catggtcgtc ttcatggtca ttcctgttgg tttcctggcg gttatacgca cggagtcgaa
     6001 aaacccgtcg ctcgtcaggc tggctcgtag ccgatggcca cgacctccca cgttctctcg
     6061 ccgccggctc cgggcaggtc gacctcgtcc cccaccgact tgcccagcag caaccgggcg
     6121 acgggggagt cgatgctgat gaactcaggc gcccggtcga actcgtccga gcccaccagc
     6181 cggtagcggg cctcttcgcc ggtctcgtcc gcaagcctca cccacgcgcc gaaaaagacc
     6241 ttgccctggt ccctcggggg gcggtccacc accgtcagct cgtccagccg cttggcgaga
     6301 aaccggatgc gggcgtcgat ctctctgagc tggcgcttgc cgtagatgta ctcggcattc
     6361 tcggaacggt cgcccagcgc ggctgcttca gcgacctttt gggtcacctc ggggcgtttg
     6421 accttccaca gctccttgag ttcggcagcc agacgctcct ggccggccgg cgtgatgtag
     6481 ggggaggatc tgggccgggg cgggcggtag cggctcatcg gcgcccgtgc gttaaggttt
     6541 ttttgccagc agacattggg tccggcctaa aggctcgcgg agagcgaggg ttcctggtag
     6601 acggtaacac cccgggagat cgtttccacc acctcgatgt ccagaatctg ctgggggtcc
     6661 acggtgaccg ggttggccga gaggatcacc agatccgcct gcttgcccgg tgtcagggac
     6721 cctttgaggt cttcctcgaa attctggtag gcggcgtgga cggtcaccgc gcggatcgcc
     6781 tccagggcgc tgatgcgctg agcctcgccc agggtctggc cgctgcgggt gatgcgattc
     6841 accgtggccc acagcagacg catcatgtcg ggcggcacca cgccgagtgc cgccatgcgg
     6901 tcgagctgat cttcccgaac cgtctgggcg tggatcatca ccgttcggtg atcgcccaga
     6961 tccatggtct cattcgcctt gtccagggcg tcgagcagca tctcggcggc agcgtcgccg
     7021 ttgcagtgca ccagcatggg gatgccgcgg ccgatgaagt gggccacccg ctcgtctgcc
     7081 atggcctgcg gaatcatggg atagccgcgg taatcgtccg gctggccgtg gggcgccacc
     7141 tcgtagggtt tgctcaggta ggccgtcttg ccctggggcg aaccatccag aagcagcttg
     7201 atgccaccga ccttcacccg gtcctggtac tcgcccagcg tgacgccttc cggaacggaa
     7261 aacgtcgggt ccatacccac ggggtacagc accacgtcga gattgacgtc cccggcgatg
     7321 gatgcttccc ggaccatctc gtggatctcc caggaagcgc ccccgtcctg gaccgcggtg
     7381 accccgttcc cggcgtacat cgcaagggcc ttgcgcaggt ccgcaccggg cgcccccccg
     7441 ccgccgccat agccgccaga atagatgtgg cgcaacggcg cggtagccgt ctcctccaga
     7501 acgccgttgg gctcgttgct gcccggctgg cggcggatca tgccgcccgg gggatcgggc
     7561 gtctcggccg tgatgccgct taagtccagc gccatggagt tcacggtggt cagatgaccg
     7621 gaaacgtgga tgagcgcgat gggatgctcg cttgagatcg cgtccaggtc ggaacgctcc
     7681 ggatggcgct gctcctcgat gagcgaatcg tcgtagccca tgccgatcac ccactggccc
     7741 ggcgtgccga aaggcgagcc ctcgggctcg aagggcgtaa tggcggcgag ctccgtggcc
     7801 gcgctaccct ggtagcc
//
LOCUS       DBNHQR010000473         6143 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_931295, whole genome shotgun sequence.
ACCESSION   DBNHQR010000473 DBNHQR010000000
VERSION     DBNHQR010000473.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6143)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6143)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6143
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_931295"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="AC16VR_10715"
     CDS             <1..592
                     /locus_tag="AC16VR_10715"
                     /inference="COORDINATES: protein motif:HMM:NF014879.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphohexomutase domain-containing protein"
                     /protein_id="HIR6011674.1"
                     /translation="VDEQGDIVDGDELLYVIAKHRATQGTLHGGIVGTLMTNYGLERA
                     LAGLNVPFERAAVGDRYVLEMLRERGWTLGGEASGHIVCLDLTTTGDAIVAALQVLTA
                     IVQSGQPLHQLKQGMSKLPQVMVNVSAPDPSRAADERALQLAVKDKSRLLAGRGRILV
                     RRSGTEPVIRVMVEGEDHAEVSAIADDLAAAVQQEG"
     gene            682..1443
                     /gene="tpiA"
                     /locus_tag="AC16VR_10720"
     CDS             682..1443
                     /gene="tpiA"
                     /locus_tag="AC16VR_10720"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008017141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HIR6011675.1"
                     /translation="MSPRRPLVAGNWKMNGSSEFVRSFAAELMARVVEDAVSVVVFPP
                     AAYLGALVRAFEGSAIEVGGQNAHMESSGAFTGEVAPEMVADVGGRWLLVGHSERRQY
                     FGESDDLIADKYAAALRACLAPVLCVGETLEEREAGEAERVVCRQLAAVTERLDIAEV
                     ARGVVAYEPVWAIGTGRTATPGQAQEMHRVIREALSERGAKLAEGMRILYGGSVNADN
                     AGELMAQPDIDGGLVGGASLSADGFAAIVAAAAGR"
     gene            1502..1858
                     /gene="secG"
                     /locus_tag="AC16VR_10725"
     CDS             1502..1858
                     /gene="secG"
                     /locus_tag="AC16VR_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008171532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HIR6011676.1"
                     /translation="MTTFETILLMLLVVDAIALAALVLLQQGRGADVGAAFGSGSSNT
                     MFGSAGSASFLTKLTVWLAIGFFLISFGLAYTAKERAAIIDEVGVPSVAPAEETVVPE
                     QLTPEPESVDSDIPDV"
     gene            1867..1953
                     /locus_tag="AC16VR_10730"
     tRNA            1867..1953
                     /locus_tag="AC16VR_10730"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1901..1903,aa:Leu,seq:gag)
     gene            2073..2149
                     /locus_tag="AC16VR_10735"
     tRNA            2073..2149
                     /locus_tag="AC16VR_10735"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2107..2109,aa:Met,seq:cat)
     gene            2411..2881
                     /gene="rimP"
                     /locus_tag="AC16VR_10740"
     CDS             2411..2881
                     /gene="rimP"
                     /locus_tag="AC16VR_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HIR6011677.1"
                     /translation="MNAKEKQIEALLTPTVEGLGCQIWGVEYASQGRHSRLRVYIERE
                     EGVSVDDCERVSRHVSDVLDVEEVISGGYTLEVSSPGMDRVLFKAEQYAANVGQHVDV
                     RLVYPFEGRKRIVGVLAGLEEDDSCGHVIVQVDDEQYLLPLESVQRTRIIPQYD"
     gene            2897..4414
                     /gene="nusA"
                     /locus_tag="AC16VR_10745"
     CDS             2897..4414
                     /gene="nusA"
                     /locus_tag="AC16VR_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HIR6011678.1"
                     /translation="MSKEMLLVVDTVSNEKGVPKDVLFGAVEAALATATKKRYDDDAE
                     FRVAIDRESGEYETFRVWTVADPEALEEEEEWNPAAQLTVEEARERDANLQLGDVIEE
                     PVESVGFGRIAAQTAKQVIVQKVREAERAQVVEAYLPRVGELVNGTVKKLGRDSVIVD
                     LGNNAEGLLTREHLIPRETFRVGDRLRALLLEVNPDNRGPQLMLTRTSPKMLIELFKL
                     EVPEIAEDVIEIRGAARDPGSRAKIAVKTNDGRIDPVGACVGMRGSRVQAVSGELAGE
                     RIDIVLWDDNPAQLTVNAMAPAEVAAIVLDEDTHAMDIAVTEDNLAQAIGRGGQNVRL
                     ASDLVGWSLNIMTEEDAAAKQEEEAVRYIEEFMRALEVDEDVATVLVEEGFTSIDEVA
                     YVPIEEMTAIEGFDEEIVEELRNRAKDALLTKAIASEELLGEKEPADDLLNMEGMERR
                     LAFQLASNDIITMEDLAELSVPDFIEIVPEVDEETAAALIMTARAPWFEEAEAEG"
     gene            4440..>6143
                     /gene="infB"
                     /locus_tag="AC16VR_10750"
     CDS             4440..>6143
                     /gene="infB"
                     /locus_tag="AC16VR_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009613042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIR6011679.1"
                     /translation="MSEVTVKQLADTVGAPVDRLLKQMQDAGLPHDSDDAAVTEEEKQ
                     TLLAYLKRSHGSDDAAPKKITLKRKTVGTLKSGQGRTGRTVNIEVRRKRTYVKRDEAE
                     TAGAAAEAPAKPASQSELEAQRIREQELARKEAEEETRRKEAERKAEEEKRVEEIKRQ
                     EEADLQARETKPGEAPAEDSAAKAAAAAAEAQAKAQASERGGKRVRGKERERPGEKRD
                     EFGDGKGRRRELSLKTQRRGKRRQPAPMPVEQQGGEFRPTDFIAREVEIPDIITVGEL
                     AQRMTVKAGEVIKALMGMGVMATINQSVDQDTATLIVEELGHKPKPIHEDAMEQALEA
                     SLQIEGDAAKRAPVVTVMGHVDHGKTSLLDYIRKTRVTSGEAGGITQHIGAYSVDTPH
                     GKISFIDTPGHAAFTAMRARGARVTDIVILVVAADDGVMPQTEEAIQHAKAAGVPMVV
                     AINKMDLEGADPDRVTNELAAKEVVPEDWGGDTQFVKVSAVSGEGIDELLEALLLQAE
                     ILELTAVEDAPGRGVVIESRLDRGRGPVSTVLVQNGTLKQGDVIIAGECHGRVRAMLN
                     DK"
BASE COUNT         1204 a         1748 c         2177 g         1014 t
ORIGIN      
        1 ggtcgacgag cagggggaca tcgtcgatgg agacgagttg ctgtatgtga ttgccaagca
       61 ccgggcgacg caggggaccc ttcatggggg catcgtcgga accctgatga ccaattacgg
      121 cctcgagcgc gcgctggccg ggctcaacgt gccgttcgaa cgggccgccg taggcgaccg
      181 gtacgtgctg gagatgctgc gcgagcgcgg ctggaccctt ggcggtgaag cgtcgggaca
      241 catcgtgtgt ctggatctca cgaccacggg cgatgccatc gtggcagccc tccaggtcct
      301 gaccgccatc gtccagagcg ggcagccgct gcaccagctc aagcagggca tgtccaagct
      361 gccccaggtc atggtcaacg tttctgcacc cgacccgtcc cgtgcggcgg atgagcgggc
      421 gctgcagctg gcggtgaagg acaagagccg cctgctcgcc gggcgtggcc ggattctcgt
      481 tcggcgttcg gggacggagc ccgtcatccg ggtcatggtc gaaggcgaag atcacgccga
      541 ggtctctgcc attgcggacg atcttgccgc cgcggtccag caggaaggct gaagccttgc
      601 aagctgctgg cgcgccgggt cgcggcgttg tctgcgtcag ccccaaagta taagattgcg
      661 cgcccttagg tggaggccgg catgtcccca cgtaggccgc tggtcgcggg caactggaaa
      721 atgaacggct cttccgagtt cgtccggagt ttcgcggctg aactcatggc ccgggtcgtg
      781 gaagatgcgg tgagcgtagt cgtgttccct cccgcggcct atctcggcgc gctggtccgg
      841 gccttcgaag ggtctgccat agaggtcggt gggcagaatg cacacatgga gtcctcgggg
      901 gctttcaccg gcgaggtcgc cccggaaatg gtggccgacg tcgggggtcg ctggctgttg
      961 gtcggtcatt ccgagcggcg ccagtatttc ggcgagtcgg acgacctgat cgctgacaag
     1021 tatgccgccg cgttgcgagc gtgccttgcc ccggtgctat gcgtgggcga gacgctcgag
     1081 gaacgcgaag ccggggaggc cgagcgggtc gtctgcaggc agctcgccgc cgtcacggag
     1141 cgattggaca tcgccgaggt ggcccggggc gtagtggctt acgagccggt atgggccatc
     1201 ggcacgggtc gcacggcgac gcctggccag gcccaggaga tgcatcgggt cattcgcgaa
     1261 gcgttgagcg agcgcggtgc aaagctggcg gaagggatgc ggattctcta cggtggcagc
     1321 gtaaacgccg ataatgccgg cgagctgatg gcccagccgg atatcgacgg cgggctggtc
     1381 ggaggcgcat cgctcagcgc ggacgggttc gcggcgatcg ttgctgcggc ggctggaagg
     1441 tgacggaaca gtgggcctgc tccggggcag gcggtgagca gaaaaatcgg taacgtgcca
     1501 catgacaact tttgaaacga ttctgttgat gctgctcgtt gtggacgcca tcgcgctggc
     1561 agcgctggtg ctgctgcagc agggccgtgg cgcggacgta ggcgcggcat tcggcagcgg
     1621 gtcctccaac accatgtttg gcagtgccgg gtcggcttcg ttcctgacga aactgacggt
     1681 gtggctggcc ataggctttt tcctgatttc tttcgggctc gcctacaccg ccaaggagcg
     1741 cgcggccatt atcgacgagg tcggggttcc cagcgtggct cctgctgagg aaactgtcgt
     1801 cccggagcag ttgactccgg aaccggaaag tgtagattcc gacatccccg atgtttgagg
     1861 ggttctgccg aagtggtgaa actggtagac acgctacctt gagggggtag tgggcgtacg
     1921 cccgtggagg ttcgagtcct ctcttcggca ccattcccgt tccagctcgg cccggcaaaa
     1981 atgtcgttta aaaccgcgat gttgcgtggt gcggcccgat atttaccggt tgcggggggc
     2041 atgagccatc aatataatgc gccacttgct gatgcggggt ggagcagtct ggtagctcgt
     2101 cgggctcata acccgaaggt cgtgggttca aatcctgccc ccgctaccaa aggataaaag
     2161 cccctcctgt gctttgcacg aaaaggtcag gggcttttta ttacgccggg gggcgcgtct
     2221 tcccggcgta tgcgcagcgg gccttatgaa cccgccatga catccttgta ggtggcccgc
     2281 cgcgtaccgg ctgggccgcc cgtggtctga tgcgaaagaa gcgatctcgc gcaattgcat
     2341 cgggtatgag aatgggcgta agcccatttt ttattggctg aaggaaagcc tgacggcgtt
     2401 tgacaagctg ttgaatgcga aggagaaaca gatcgaagcg ttgctgacgc ccacggtaga
     2461 agggctgggc tgccagatct ggggggtcga gtacgcctcc cagggtcggc attcgcgtct
     2521 gcgtgtctac atcgaacgcg aggaaggcgt ttcggtggac gactgcgagc gtgtgagccg
     2581 acatgtcagc gacgttctgg atgtggaaga ggtgatatcc ggcggctata cgctggaggt
     2641 gtcctctccg ggcatggatc gggtcctgtt caaggcggag cagtacgcgg cgaacgtcgg
     2701 ccagcacgtg gatgtccgtc tcgtatatcc gttcgagggg cgcaaacgga tcgtaggggt
     2761 gttggcaggc ctggaggaag acgattcctg cggacacgta atcgtgcagg tggatgatga
     2821 acagtacctg ttgccgctgg agagcgttca gcgcaccagg attattcccc agtatgactg
     2881 aggtgcgacc aaagttatga gcaaagagat gttgttggtg gtggataccg tctccaacga
     2941 gaagggcgtc ccgaaggacg tgcttttcgg cgccgtggag gcagcgctcg cgactgccac
     3001 caagaagcgc tacgacgacg acgccgagtt ccgcgtcgcc atcgaccggg agtctggaga
     3061 gtacgagacg ttcagagtct ggaccgtggc ggatccggag gcgctggaag aagaggaaga
     3121 gtggaacccc gcggctcagc tgaccgtgga ggaagcccgg gaacgggatg cgaatcttca
     3181 gctcggcgat gtgatcgagg agccggtgga gtcggtgggc tttggccgta tcgctgcgca
     3241 gacggccaag caggtcatcg tgcagaaggt ccgtgaggcc gagcgtgctc aggtcgtcga
     3301 agcttacctg ccgcgggtcg gggagctcgt taacggaacc gtgaagaaac tcgggcgtga
     3361 cagcgtcatc gtagatctcg gcaacaacgc cgagggcctc ctgacccggg aacacctgat
     3421 tccccgggaa acgtttcgtg tgggtgaccg gttgcgcgcg cttctgctcg aggtcaatcc
     3481 ggacaatcgc ggacctcagc tcatgctgac gcgcacctcg ccaaagatgc tcatcgagct
     3541 tttcaagctg gaggttcccg agatcgccga ggacgtcatc gagatacggg gcgcggcgcg
     3601 ggatccgggg tccagggcga agatcgcggt gaagaccaac gacggtcgga tcgatccggt
     3661 aggcgcctgc gtcggcatgc ggggctcccg cgtgcaggcc gtatccggtg agctggccgg
     3721 ggagcggata gacatcgttc tttgggacga caacccggcc cagcttaccg tcaacgccat
     3781 ggcgccggcc gaggtggccg ccatcgtgct cgatgaggac acccacgcca tggatatcgc
     3841 ggtcaccgag gacaacctcg ctcaggcgat cggccgcggc gggcagaatg tgcgtctggc
     3901 cagtgatctg gtcggctggt cgctgaacat catgacggaa gaggatgcgg cggcgaaaca
     3961 ggaagaggag gccgtgcgct acattgagga gttcatgcgc gccctcgagg tcgacgagga
     4021 cgtcgccacc gtgctggtgg aagaaggttt cacctccatc gacgaggtcg cctacgtgcc
     4081 catcgaggag atgacggcca ttgaagggtt cgacgaggag atcgtcgagg agctgcgcaa
     4141 ccgggccaag gacgctctgc tgacgaaggc gatcgccagt gaggagctgc tcggcgaaaa
     4201 ggagcctgcg gacgatctgc tgaacatgga aggcatggag cgacgcctgg cgttccagct
     4261 ggccagcaat gacatcatca cgatggaaga tcttgctgaa ctttcggtgc ccgatttcat
     4321 cgagatcgtc ccggaggtcg acgaagagac cgcggctgct ctcatcatga cggcccgcgc
     4381 gccctggttc gaagaagcgg aggcggaagg gtaagccgcc ccgaagagga aaaagagtca
     4441 tgtcggaagt cacagtcaag caactcgcag atacggtggg ggcgcccgta gaccgtctgc
     4501 tcaagcagat gcaggatgcc gggcttccac acgacagcga cgacgcggcg gtcacggaag
     4561 aggagaagca gacgctgctc gcctacctca agcgcagcca cggatctgac gacgccgcgc
     4621 caaagaagat cacgctgaag cgaaagaccg tcggaaccct caagtccggc cagggcagga
     4681 cgggccgtac cgtgaacatc gaggtgcgcc gtaagcgcac ctacgtgaag cgggacgagg
     4741 ccgagaccgc gggcgcggcg gcagaagcgc cggcaaagcc ggccagccag tctgagctcg
     4801 aggcgcagcg tattcgggag caggaactcg ctcgcaaaga ggccgaagag gaaacccggc
     4861 gcaaggaagc ggagcgcaag gccgaagagg aaaagcgggt agaggagatc aagcgtcagg
     4921 aggaagccga ccttcaggcg cgcgaaacca aacccggcga ggctccggca gaagactcag
     4981 cggcgaaagc ggccgctgcc gctgcagagg cccaggcgaa ggcccaggca agcgagcgcg
     5041 gcgggaaacg cgtacgcggc aaggagcgcg agcggccggg cgaaaaacgc gacgagttcg
     5101 gcgacggcaa aggccgccgc cgggagctct cgctcaaaac gcagcggcgt ggcaaacgtc
     5161 gtcagcccgc accgatgccc gtcgagcagc agggcgggga attccgaccg acggacttca
     5221 tcgcgcggga ggtcgagata cccgacatca tcacggtggg cgagctggcc cagcgcatga
     5281 cggtgaaggc aggcgaagtc ataaaagcgc tcatgggtat gggcgtgatg gcgacgatca
     5341 accagagcgt cgatcaggat accgccacgc tcatcgtcga ggagctgggc cacaagccga
     5401 aacccatcca cgaggatgcc atggagcagg ctctggaagc ctcgctgcag atcgagggcg
     5461 atgccgcgaa gcgggcaccc gtggtgacgg tcatggggca cgtcgaccat ggcaagacct
     5521 cgctgctgga ctacatccgc aagactcggg tcaccagcgg cgaagctggt gggatcaccc
     5581 agcacatcgg cgcctacagc gtggacacgc cgcacggcaa gatcagcttc atcgatacgc
     5641 ccggtcatgc cgcctttacc gccatgcggg ctcgtggcgc cagggttacg gacatcgtca
     5701 tcctggtcgt ggctgcggac gacggcgtca tgccccagac cgaggaagca atacagcacg
     5761 ccaaagcggc cggcgtgcct atggtcgtgg ccatcaacaa gatggacctc gaaggcgccg
     5821 acccggaccg ggtcacgaac gagctggcgg ccaaggaagt ggttcccgag gattggggcg
     5881 gcgacactca gttcgtcaag gtctcggcgg tctccggcga aggcattgac gagcttctcg
     5941 aggcgttgct cctgcaggcg gaaatcctgg agctgacggc cgtggaggat gctcccgggc
     6001 gcggcgtcgt catcgaatcg cggctcgatc gcggtcgggg gccggtgtcc accgtcctgg
     6061 tgcagaacgg caccctcaag cagggggacg tgatcattgc gggcgaatgc cacggccgcg
     6121 ttcgtgccat gctcaatgac aag
//
LOCUS       DBNHQR010000474         1780 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1628570, whole genome shotgun sequence.
ACCESSION   DBNHQR010000474 DBNHQR010000000
VERSION     DBNHQR010000474.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1628570"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="AC16VR_10755"
     CDS             complement(<1..240)
                     /locus_tag="AC16VR_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011680.1"
                     /translation="MTRHLLFAAAGLLLAACSTVSIDGPTAPRGDPAAGRLTLPSGRT
                     VWVYPNDLAFTGQARYEWPDGRAYAGGWLSGRPHGT"
     gene            319..1353
                     /locus_tag="AC16VR_10760"
     CDS             319..1353
                     /locus_tag="AC16VR_10760"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HIR6011681.1"
                     /translation="MTASKDDGSIEGVDIPRVTHWLVGRIAGLRPPLEFSLIAGGHSN
                     LTYRFADQAGAEYVLRRPPLGHVLESAHDMAREHRIVSALAGTEVPVAPTHGLCENRD
                     VNGAPFYVMSYVRGAVLHDADAARELDPAERHALGEDVINVLARLHALDPDAVGLGTL
                     GRKEAYLARQLKRWKKQWEASKTHEIPEMEETARLLEENMPEQIGAAIVHGDYRLGNM
                     IVADGRILAVLDWELCTLGDPLADVGYLLNNWIQPEDGAGELVPTAAGGFPSREALCD
                     GYTAATGRDLSGINYYRAFSHWRLAAIGQGVYKRYLVGAMGERPDMDLDTYKASVQQR
                     AAAALELLTA"
     gene            complement(1350..>1780)
                     /locus_tag="AC16VR_10765"
     CDS             complement(1350..>1780)
                     /locus_tag="AC16VR_10765"
                     /inference="COORDINATES: protein motif:HMM:NF025701.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HIR6011682.1"
                     /translation="ALYGCSGRDDVSLVEVLNHDQCRMSQAGTEVVSIEDVARIRGGR
                     LLSAPDEPATTPTDLLLVAVSRGQQPTPGYGFELVAGTMKGDAVTVRLAWNQPPADAV
                     LAQVITNPCIVIGLEGEGFSRLRVIDQDDTPLGEIVLPGG"
BASE COUNT          296 a          579 c          617 g          288 t
ORIGIN      
        1 ggtgccgtgt ggcctgccgg acagccagcc gcccgcatag gcgcgcccgt cgggccattc
       61 gtagcgagcc tgcccggtga atgccagatc gttcgggtag acccacacgg tgcgcccgga
      121 gggcagcgtg agcctgccgg cagcgggatc gcctcggggg gcggtgggac cgtctatgga
      181 gaccgtagag catgccgcca ggagcagacc tgcggcagcg aagagcagat gtcgcgtcac
      241 tgggttatgg aaaacgatct catgtcaaaa ctcaaaggct ttgggtaacc ttgcccgggt
      301 ctggaacggg agaattccgt gaccgcgagc aaagacgacg gcagcatcga aggcgtcgac
      361 atcccgcgcg tgacccattg gctggtaggc cggatcgccg gcctgcggcc gccgctggag
      421 ttttcgctga ttgcgggcgg tcactcgaac ctcacctacc gctttgccga tcaggctggc
      481 gccgagtacg tgctgcgtcg cccgcccctg ggccacgttc ttgaaagtgc tcacgacatg
      541 gcccgggagc atcgtatcgt ctccgcgctc gcgggcaccg aggttcccgt ggcgcccacc
      601 catggccttt gtgagaaccg ggacgtcaac ggggcgccct tctacgtgat gagctacgtc
      661 cggggggccg tgctgcacga tgcggacgcc gcccgtgagc tcgatccggc cgagcggcat
      721 gcccttggcg aggatgtgat caacgtgctc gccaggctcc atgccctgga tcccgacgca
      781 gtgggtctcg gcacgctggg gcgcaaggaa gcctaccttg cccggcagct caagcgctgg
      841 aagaagcagt gggaggcatc gaagacccac gagattcctg agatggagga gacggccagg
      901 ctgctcgagg aaaacatgcc cgagcagatc ggggcggcca tcgtccacgg tgactatcgc
      961 ctgggcaaca tgatcgtcgc cgacggccgt attcttgccg tcctcgactg ggagctctgc
     1021 acgctgggcg acccgctggc ggacgtcggc taccttctga acaactggat tcagcccgag
     1081 gacggcgcgg gtgagctcgt gccgacggcg gccggggggt tcccgtcccg ggaggccctc
     1141 tgtgacggtt acacggcggc gacggggcgg gacctctcgg ggatcaacta ctaccgggct
     1201 ttttcccact ggcgtctggc cgccatcggt cagggcgtct acaagcgata cctggtgggc
     1261 gccatgggcg agcgcccgga catggacctg gacacctaca aggcgagcgt ccagcagcgg
     1321 gcggccgccg cgctcgagct gctgacggct tagccgccgg gcagcacgat ctcgcccagc
     1381 ggggtgtcgt cctggtcgat gacgcgcagc cggctgaagc cctcaccttc cagcccgatg
     1441 acgatgcagg gattggtgat cacctgagcg agcacggcat ccgcaggcgg ctggttccag
     1501 gccagccgta ccgtgaccgc atcgcctttc atggtccccg cgacgagctc gaagccgtaa
     1561 cccggcgtgg gctgctggcc tctggagacc gcgacgagca gcaggtccgt gggcgtggtg
     1621 gcgggctcgt caggggcaga cagcagcctg cccccgcgga tgcgcgccac atcctcgatg
     1681 ctcaccactt ccgttccggc ctgggacatc ctgcattggt cgtggttgag cacttccacc
     1741 aaagagacgt cgtcacgacc gctgcagccg taaagcgcgg
//
LOCUS       DBNHQR010000475         2690 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_868202, whole genome shotgun sequence.
ACCESSION   DBNHQR010000475 DBNHQR010000000
VERSION     DBNHQR010000475.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_868202"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2024)
                     /locus_tag="AC16VR_10770"
     CDS             complement(<1..2024)
                     /locus_tag="AC16VR_10770"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HIR6011683.1"
                     /translation="MKTIAGADAPFEICWDHLGIPHVYASTVADAYRGMGYVEGYERL
                     WQIHLSCLFANGNAAADMGERFVAQDALHRAFGVHASYDLPESPGDWVVDAYLEGLNA
                     YVDTLDDPPPEFRHTGALPRRFTRADVAARYRFTNFFQHRSWPNKLMLGRLMATHGVE
                     WFRGHLLRFSDEDEKLIELLREPLKSLNLRPINLLFPEAPLSGSNNWVVTGRLSASGK
                     PMLGMDPHQAHSIPNTFFYVHLHAPGWDVFGASFPGVPYFMMGYTKKIAWGLTTGFVD
                     NYDVYVEEIAGDRYRTPDGWAEVERRTETIGVRGGTTREVEIASTRHGPLLEPLTDAL
                     EMTANGPRRYQTAVSWNLTHLETSAGALALLPLAEDAGGFGGALFEGDVCPLVNNIIC
                     VDTKDDIRRFVAATLPARKGVTGMVPLPGWDPDCDFRWSRADELLVEQAPASGFALTA
                     NNDTMGDAGPFPIHNFPTLPARAERIRDLLEAGGSADGKFTPADFERMQLDLYDYRAR
                     DQIPGILMALEGREDDDLELAAAILRGWDCQATGESAAACLYYPFVDRVWQRSFMMAV
                     LDDDVLRGIPLAAPGLNRFDVDAFLAEGSPWRAHRELLFLTMAEVMKGVVQDVRKDLG
                     EPDTWAWGRLHQVRFWHSLRKHEPWKDMQVGPDPVGGSGTTLAMALHVGNG"
     gene            2168..>2690
                     /locus_tag="AC16VR_10775"
     CDS             2168..>2690
                     /locus_tag="AC16VR_10775"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6011684.1"
                     /translation="MLKTENYRTMLVERRENGVGVLTLNRPERLNAVNGTMHSELARF
                     SLDFASDPDLRVLVVTGAGRAFCAGGDFSPDDPVGSNPDGTDMWAEARQIVDRMLECE
                     KPIISAVNGYAMGLGATVALLADVVVVSPKAVIADTHVAMGIGAGDGGQLIWPHLIGV
                     NRAKYYLMSGDRID"
BASE COUNT          458 a          848 c          914 g          470 t
ORIGIN      
        1 ccgttcccca cgtgcagggc catggccagg gtcgtgccgc tgccgccgac gggatccggt
       61 cccacctgca tgtctttcca gggctcgtgc ttgcgcaggc tgtgccagaa acgcacctga
      121 tgcagccggc cccaggccca ggtgtcgggt tcgccaaggt cttttctcac gtcctgcacg
      181 acgcctttca tgacctcggc catggtcagg aacagcagtt cccggtgcgc gcgccatggt
      241 gatccctcgg cgagaaacgc gtccacgtcg aagcggttga ggcccggcgc cgccagcgga
      301 atgccccgaa ggacgtcgtc gtccagcacg gccatcatga agctccgctg ccagactcgg
      361 tccacgaacg ggtagtagag gcatgcggcc gccgactctc cggttgcctg gcaatcccag
      421 ccgcgcagga tggccgcggc gagctcgagg tcgtcgtcct cgcgaccttc cagcgccatg
      481 aggatcccgg gaatctgatc ccttgcccgg tagtcgtaga ggtccagctg catgcgctcg
      541 aaatcggccg gggtgaactt cccgtccgcg gagccgcccg cttcgagcag gtcgcgaatc
      601 cgctccgcgc gggccggcag cgtcggaaag ttgtggatgg gaaagggccc cgcatcgccc
      661 atggtgtcgt tgttcgccgt cagcgcgaag cccgaggcag gcgcctgctc gaccagaagc
      721 tcatcggctc gcgaccaccg aaagtcgcag tcgggatccc atccgggaag cgggaccatc
      781 cccgtcacgc ccttgcgtgc gggcagggtc gccgcgacga agcgcctgat gtcgtccttg
      841 gtgtcgacgc agatgatgtt gttgacaagc gggcagacgt cgccttcgaa cagcgcgccg
      901 ccgaatccgc cggcatcctc ggcgagcggc agcagggcaa gcgcgccggc cgacgtctca
      961 agatgagtga ggttccagct caccgcggtc tggtagcgcc gcggcccgtt ggcggtcatt
     1021 tcgagcgcgt cggtcagggg ctcgagcagc ggtccgtgac gcgtcgaggc aatctcgacc
     1081 tcccgcgtgg tgccgccccg gaccccgatg gtctcggtcc gcctttccac ctcggcccag
     1141 ccatcgggcg tgcggtagcg gtccccggcg atctcctcga cgtagacgtc gtagttgtcc
     1201 acaaacccgg tggtgagtcc ccaggcgatc ttcttcgtgt agcccatcat gaagtagggc
     1261 acgccgggaa acgaagcgcc gaacacgtcc cagccgggcg cgtggaggtg cacgtagaag
     1321 aacgtgttgg gaatggaatg agcctgatgc ggatccatcc ccagcatggg ctttccggaa
     1381 gcggacagcc tgccagtcac cacccagttg ttgctgccgg acaacggcgc ctccggaaag
     1441 agcaggttga tgggcctgag gttgagcgat ttgagaggct cacgcagcag ctcgatgagt
     1501 ttctcgtcct cgtcggagaa gcgcagcagg tggccgcgga accactccac gccgtgggtg
     1561 gccatcagcc ggccgagcat gagcttgtta ggccacgacc ggtgctggaa gaagttggtg
     1621 aatcggtagc gcgcggcgac gtcggcgcgc gtgaagcgcc ggggcagggc gcccgtgtgg
     1681 cggaattcgg ggggagggtc atcgagggtg tcgacgtagg cgttcaatcc ctcgagatag
     1741 gcgtccacca cccagtcacc gggactctcg ggaagatcgt agctggcatg cacgccgaag
     1801 gcgcgatgca gggcgtcctg agccacgaac cgctctccca tgtcggcggc ggcattgccg
     1861 ttggcgaaca ggcagctcag gtgaatctgc cacaggcgct cgtagccctc cacgtagccc
     1921 atgccccggt aggcgtcggc gaccgtggag gcatatacat gggggatgcc gaggtggtcc
     1981 cagcagatct cgaacggggc atctgctccg gcgatggtct tcatgctggt cttcctctat
     2041 cgcgcgctcg ggtgtgggtc caggggatga ttcattctct gtcggatcgt ttcgccgtag
     2101 agtagcggat cgaagatggt ggtgcaggtt tcaacaaagc tggctgacaa ctcggaggac
     2161 atctcacatg ctcaagacgg aaaactatcg gaccatgctc gtcgagagac gggagaacgg
     2221 cgtcggtgtg cttacgctca atcgccccga gcgtctcaac gcggtgaacg gcaccatgca
     2281 ttcggagctt gcgcgcttca gcctggactt tgccagcgac ccggacctgc gcgtgctggt
     2341 cgtcactggc gcgggccggg cgttctgcgc gggaggcgat ttcagccccg acgacccggt
     2401 gggttcaaat cccgatggga ccgacatgtg ggcggaagcc cggcagatcg tcgaccgcat
     2461 gctggaatgc gaaaaaccca tcatctcggc cgtcaacggg tacgccatgg ggcttggcgc
     2521 taccgttgcg ctgctggccg acgtcgtggt cgtgtctccc aaagccgtga ttgcggacac
     2581 ccacgtggcc atgggcattg gcgcgggtga cgggggccag ctcatctggc cgcacctgat
     2641 tggtgtgaat cgcgccaagt actacctgat gtcgggggat cggatcgacg
//
LOCUS       DBNHQR010000476         2393 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1373571, whole genome shotgun sequence.
ACCESSION   DBNHQR010000476 DBNHQR010000000
VERSION     DBNHQR010000476.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1373571"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1102
                     /locus_tag="AC16VR_10780"
     CDS             <1..1102
                     /locus_tag="AC16VR_10780"
                     /inference="COORDINATES: protein motif:HMM:NF016251.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HIR6011685.1"
                     /translation="SVLPTALRAFDRDAPKVRRVGLLLPLSGPLAVAGRAVRDGYIAA
                     YLHESAQSEQEIIVYDTAAAPLPSLYEKALLDEVDVLIGPLTKEEASALSGLAPSVPT
                     LVLNYLEDGQRAPSNVYQLGLAIEDEADTMVSRLLEDGAERLLVVHNDQDWSRRAARR
                     LTSSWPYPVTREFFPDVKTITESVGRAMQIDASLERRDELASLLGTDLEFLPRARGDL
                     DAVVALVDTVEANALVPALRFHFAQDLPVYASSQTVRGATAQELGELAGFRVSELPWI
                     LFSDPIYLAMADVFLLQGNRFAALYALGVDAYRVSGRLTLLASGGVHQLLGSTGLLTV
                     DGSGRFRRELAWGLIRRGRLEPLPVMAGQSAL"
     gene            1099..1464
                     /locus_tag="AC16VR_10785"
     CDS             1099..1464
                     /locus_tag="AC16VR_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HIR6011686.1"
                     /translation="MTPQARAGAGAESAALDHLRARGLELVARNFRCRLGELDIVMVD
                     GNELVFVEVRLRNHARFGDGAASITAAKRQKLARAARAFLQRHPRYQSCPCRFDVVAI
                     KGEGQRMEMRWLKNAFAVG"
     gene            complement(1517..1909)
                     /locus_tag="AC16VR_10790"
     CDS             complement(1517..1909)
                     /locus_tag="AC16VR_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HIR6011687.1"
                     /translation="MKAKAKRPYLLRALYDWLVDSSLTPYVLVSAEDEAAMVPADYVN
                     DGKIVLNISPSAMRGLVIDDAGLSFDGRFGGAPYPVSAPISSVLAIYAKETGEGMIFD
                     PEEFPSRTDDSPEAPKDRPSGPELKVIK"
     gene            complement(1928..>2393)
                     /locus_tag="AC16VR_10795"
     CDS             complement(1928..>2393)
                     /locus_tag="AC16VR_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007162413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HIR6011688.1"
                     /translation="TLPTLLDRDLVLYEANVIMEYLDERFPHPPLLPVYPVQRALSRL
                     WITRVEKEWSGRLDILMAGKGRETTITKARKELRESIISIAPIFSEKPFFMNDEFTLV
                     DCCVAPILWRLKYVGINLPERSTRPLQRYMQVMFERSSFRESLSEAELEMRD"
BASE COUNT          407 a          777 c          788 g          421 t
ORIGIN      
        1 ttccgtcttg cccacggctc tccgcgcctt cgaccgggac gccccgaagg tgcgacgggt
       61 cgggttgctg ctgccgctca gcggccccct cgccgttgcc gggcgcgccg ttcgggacgg
      121 ctacatcgcc gcctatctgc acgagtctgc ccagagcgag caggagatca tcgtctacga
      181 cacggccgcc gcaccgctgc cgagcttgta cgagaaggcc ctcctggacg aggtggacgt
      241 gctcataggg cccctgacga aggaagaggc gtccgccctc agcgggctcg ccccgtccgt
      301 tcccaccctg gtgctcaact atctcgaaga cggccagagg gcgccctcga acgtctacca
      361 gctcgggctt gccatcgagg acgaggccga caccatggtg agcaggctcc tggaggatgg
      421 cgcagagcgc ttgctggtcg tccacaacga tcaggattgg tcgcgtcgag cggcgcggcg
      481 acttacttcc agctggccct atccggtcac gcgcgagttc tttccggacg tcaaaaccat
      541 caccgaatcc gtcggacggg ccatgcagat cgacgcgagc ctcgagcgcc gcgacgagct
      601 ggccagtctc ctggggaccg acctggagtt tctgccacgc gccagggggg acctcgacgc
      661 cgtggtcgcg ctcgtcgata ccgtcgaggc caatgccctc gtgcctgcgt tgcgctttca
      721 ttttgcccag gatctgcccg tgtacgcgag ctcgcagacg gtgcgtggag caacggcaca
      781 ggaactcgga gagcttgccg ggtttcgcgt cagcgagctg ccctggatat tgttctccga
      841 ccccatctac ctggccatgg ccgacgtgtt cctcctgcag ggcaatcgct tcgccgcctt
      901 gtacgcgctt ggcgtggacg cttatcgcgt gagcgggcgc ctgacgctgc tggcctccgg
      961 cggggtccac cagctgctgg gcagcacggg cctgctcacg gtggatggct ccggacggtt
     1021 ccgtcgggag ctggcgtggg gactgatccg caggggccgc ctcgaaccgc tcccggtgat
     1081 ggccgggcaa tcggccctct gacgccccag gcacgggcag gtgccggggc agaatcggcc
     1141 gcgctggacc atctccgcgc gcggggcctc gagctggtag cccgcaattt ccgctgccgt
     1201 ctgggcgagc tggacatcgt catggtggac ggcaacgagc tggttttcgt cgaagtccgt
     1261 ctgcgcaacc acgctcgctt cggcgacggc gcggcgagca tcaccgccgc aaagcggcag
     1321 aagctggcaa gggccgcccg ggcatttctc caacgccacc cccgatacca gagctgccct
     1381 tgccggttcg acgtggtggc catcaaagga gagggtcaac gcatggagat gcgatggcta
     1441 aaaaacgcgt tcgcggtcgg gtaatgattc gatcctgccg ccagctggcg cggtgaagca
     1501 atgagaaatg cagccgctac ttgatgacct tcagctcggg gccggacggg cggtccttcg
     1561 gcgcttccgg ggaatcatcg gtacgggaag gaaactcctc cgggtcgaag atcatgccct
     1621 cgccggtctc cttggcgtag atggccagaa cactcgagat gggcgcggat accggatagg
     1681 gcgctccacc gaaccgcccg tcgaagctga gcccggcgtc atcgatgaca agtcctcgca
     1741 tggccgacgg cgatatgttg agaacgatct tgccgtcgtt gacgtagtcg gccggcacca
     1801 tcgccgcttc gtcctccgcg gagacgagca cgtacggggt gagcgaagag tcgacgagcc
     1861 agtcgtaaag ggctcgaagc aggtaaggac gtttcgcctt ggctttcacg ccagaccgcc
     1921 cccggggtca gtcccgcatc tcgagctcgg cctcggagag gctttctcgg aacgagctgc
     1981 gctcgaacat gacctgcatg tagcgctgca ggggacgcgt cgagcgttcc ggcaggttga
     2041 tgccgacgta cttcaatcgc cacaggatgg gcgccacgca gcagtcgaca agcgtgaact
     2101 cgtcgttcat gaaaaaaggc ttctcgctga aaatcggcgc gatgctgatg atgctttcgc
     2161 gcagttcctt gcgcgccttg gtgatggtcg tctcgcgacc tttgccggcc atcagaatat
     2221 ccagccggcc gctccactcc ttctccacac gcgtgatcca aagccgcgac agagcgcgct
     2281 gaacgggata gacgggcagc agcggcgggt gcggaaaccg ctcgtccaga tattccatga
     2341 tcacgttggc ctcgtagagc acgaggtcgc ggtcgagcag cgtcggcaag gtg
//
LOCUS       DBNHQR010000477         1620 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_615774, whole genome shotgun sequence.
ACCESSION   DBNHQR010000477 DBNHQR010000000
VERSION     DBNHQR010000477.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_615774"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="AC16VR_10800"
     CDS             complement(<1..656)
                     /locus_tag="AC16VR_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen
                     decarboxylase/cobalamine-independent methonine synthase
                     family protein"
                     /protein_id="HIR6011689.1"
                     /translation="MSADRILTTHTGSLPRPDDLIQMMWAKGDGIPVDEGALAERVAA
                     AVQETVDKQVAAGISIVNDGEMSKPSYATYVKDRLSGFGGESPERYYFADLDAYPRSA
                     EIVAANPGRRKRSAPACNAPIGVQDAEAAALDMVNLCRSAEGADVEGIFSSAASPGVV
                     SLFFGNEYYASDEEYVFAIAEAMRHEYEAIAAAGATVQLDCPDLAMGRHTSYADMDLA
                     A"
     gene            801..1448
                     /locus_tag="AC16VR_10805"
     CDS             801..1448
                     /locus_tag="AC16VR_10805"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR6011690.1"
                     /translation="MGKISLTIDNGPHPTVTPEVLDALADRQVTATFFLIGQEAARPG
                     GLDLIEHISKAGHPIGNHTWSHTVAFGLNPGASAIADEVARTQKLIAPFASQPPMFRP
                     AGGGGRLDSSLLSAELIDYLCRERYTCALWNVVPRDWEDPQGWVNRALREVGTLDWSV
                     VVVHDVIDGNADQIAAFIDRARDDGHEFVTDIAPECLPIVGGTITADLAALTGSR"
BASE COUNT          271 a          558 c          521 g          270 t
ORIGIN      
        1 gcggcgagat ccatgtccgc ataggaggtg tgtcttccca tggcgagatc ggggcagtcg
       61 agctggaccg tggcgccggc ggcggcaatg gcctcgtact cgtggcgcat ggcctcggcg
      121 atggcgaaca cgtactcctc atcggaggcg tagtactcgt taccgaagaa cagggagacc
      181 acacccggcg aggcggcgct ggagaagatc ccctccacgt cggcgccttc agccgaccgg
      241 cacaggttga ccatgtccag agcggctgct tcggcgtcct gcacgccgat cggcgcgttg
      301 caggccgggg ccgagcgctt gcgccggccg ggattggcgg caacgatctc cgcgctccgg
      361 ggatacgcat cgaggtcggc gaagtagtag cgctccggag actcaccgcc gaacccgctg
      421 agtcggtcct tgacgtaggt ggcgtagctg ggtttcgaca tctcgccgtc gttgacgatg
      481 ctgatacccg ccgccacctg tttgtccacg gtctcctgga cggccgcggc gacccgctcg
      541 gcaagcgccc cctcgtctac cgggatgccg tcccctttgg cccacatcat ctgaatgaga
      601 tcatccgggc gcggcaggct gcccgtatgg gtcgtgagaa ttcggtctgc gctcatgtgc
      661 ttccccgtgg ccttccgccc gaagattagc tcatctgcaa agcggctccc agccgcagcc
      721 ggtggccgcc gaaactcggc aagcgccttc tgctaagatc ccccgactct cgagcgaccc
      781 aaccggccag gaccaaatcc ttgggcaaga tctccctgac aatcgacaac ggaccgcatc
      841 cgacagtcac tcccgaagtg ctcgacgcgc tcgccgacag gcaggtcacc gccacgttct
      901 ttctgattgg ccaggaggcg gcacgacccg gcgggctgga tctcatcgag cacatcagca
      961 aggcgggcca tccgatcggc aatcacacct ggagccacac cgttgcgttc ggcctgaacc
     1021 cgggcgcgtc ggccatcgcc gacgaggtcg cccgaacgca gaagctgatc gcgccctttg
     1081 cgagccagcc acccatgttc cgtcccgccg gtggcggcgg gcggctagac tcgagcctcc
     1141 tctcagcgga gctcatcgac tacctctgcc gcgagcgcta cacctgcgcg ttgtggaacg
     1201 tggtgccccg agactgggaa gacccgcagg gctgggtaaa ccgggcgctt cgagaggtcg
     1261 gcacgctgga ctggagcgtg gtggtcgttc acgacgtcat cgacgggaac gccgatcaga
     1321 ttgccgcctt catcgaccgg gcccgggatg acggccacga gttcgtcacg gacatcgcgc
     1381 ccgagtgcct gcccatcgtg ggagggacca tcaccgccga cctggcggcc ttgaccggct
     1441 cacgttagaa ccatctcctg cgggccgcgg cgtttacggg taaccggctg gagccaccac
     1501 ctccaaccgc ccgtgacgcg gcaggtacag cgccgttttc acggggcggt cctcgagctg
     1561 ctcgaaaagc cgccggtagc cgtcgagctg ttcctgatag cgctcgcgct cctgggcgaa
//
LOCUS       DBNHQR010000478         2975 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_425167, whole genome shotgun sequence.
ACCESSION   DBNHQR010000478 DBNHQR010000000
VERSION     DBNHQR010000478.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_425167"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="AC16VR_10810"
     CDS             complement(<1..227)
                     /locus_tag="AC16VR_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011691.1"
                     /translation="MDPWTLGIFLGGALFGSYVQSVTGFAMAMILVAVASAAGNSQVS
                     VVAAVVSLLALVNVALALRGHLERVEARLFV"
     gene            309..1085
                     /locus_tag="AC16VR_10815"
     CDS             309..1085
                     /locus_tag="AC16VR_10815"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6011692.1"
                     /translation="MDLGLTGKTALVSGSYRGTGLVIARHLAAEGVRVFVHGLEPGQA
                     ERAVEEIGSGDPVTGDITCDAGAREVAEALGDSRVDILVNNYGTADRGTWQDSDTGDW
                     LDAYQKNVLSAQRLIRAYFAPMRERGWGRIINLGTVGSTRPNSRMPHYYASKGALATM
                     TISLAKEASGTGVRVNLVSPGLILTPEVEAAYLERGRAKGWGETWEEVEPNVAADIPI
                     GRIVRREEVADLVTFLASPLADAIHGQNIRVDGGALDVLS"
     gene            1082..1468
                     /locus_tag="AC16VR_10820"
     CDS             1082..1468
                     /locus_tag="AC16VR_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011693.1"
                     /translation="MTGPGLTRPGIRLLGLAALGLCVLAYDAGSYTLLHRLVLPIAMG
                     VAAGLMVQSAAAVLLAGTLLAGVHARPADPDWVIGIAYPALAALCGVGLTWIVVLRFR
                     RRIEATREARWRGRRERSAAPEGSES"
     gene            1470..2321
                     /locus_tag="AC16VR_10825"
     CDS             1470..2321
                     /locus_tag="AC16VR_10825"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6011694.1"
                     /translation="MGTRCFLVSGTTSGIGLATARALAATGGTVVTANRDPDRGESTA
                     ALLRDATSNPNVINTVCNLSSLASVRSCAARIREQFPRIDVLINNAGVWTAKPQLSED
                     GFELSFATNHLGPFLLTHMLLEPLRAAGGARIVNVASVQHRAADLDLDHLTPKTPYRG
                     LRAYSRSKLANVMTTLAFARRFDAAEISANCLHPGVVASNIVPENSPLLRRAARVVSG
                     LMRSPARGAATSIYLATSPEVTGVTGKYFDPDQRIIQPSAEARDEAAQDLLWERSLEL
                     TGLAEAG"
     gene            2323..>2975
                     /locus_tag="AC16VR_10830"
     CDS             2323..>2975
                     /locus_tag="AC16VR_10830"
                     /inference="COORDINATES: protein motif:HMM:NF042569.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase kinase/phosphatase AceK
                     regulatory subunit"
                     /protein_id="HIR6011695.1"
                     /translation="MTRGKSAMASAADGIARAIAMGYESYQAEFQAITAMAQSRFEGQ
                     DWAGVQDAGARRLAIYGEHVRDVVAEVHTLTGGSEIDASLWRKSKQAFTSIISEHYNA
                     ELYETFYNSIHREMTDDSVVDDTEMFVRTLFPIPPVQPAEPVFERHAIAGEPEEFVRT
                     LLDGLAINRPWVDRDRDVRNILRSLSEERPEIQDPLRLDVDILKPIFYRNKGAYVVGR
                     "
BASE COUNT          545 a         1008 c          973 g          449 t
ORIGIN      
        1 cagacgaaaa gacgcgcctc cacccgctcg agatgaccgc gcagcgcgag cgccacgttc
       61 acgagagcga gcaggctgac caccgccgcc accaccgaga cctgagaatt gccggcggcg
      121 ctggccacgg ccaccaggat catggccatg gcgaagccgg tcacggactg cacgtagctg
      181 ccgaacaacg cgccgccaag gaagatgccg agagtccagg ggtccatcgc ggacggcagt
      241 ataggcgatc gcggcagatc gggtggtata gtcccggcct ccatcggggg cacgacggaa
      301 aagcaggaat ggatctcggc ctcacaggca agacggcgct cgtcagcggc agctatcggg
      361 ggaccgggct cgtcatcgcc cgccatctgg cagccgaggg cgtgcgggta ttcgtccacg
      421 ggctggagcc cggtcaggcg gagcgcgccg tggaggaaat cggcagcggc gacccggtga
      481 ccggcgacat cacctgcgat gctggcgccc gagaggtggc cgaggccctg ggcgacagcc
      541 gcgtcgacat acttgtcaac aactacggaa ccgcggaccg gggtacctgg caggatagcg
      601 acaccggcga ctggctggac gcctaccaga aaaacgtgct ctccgctcag cgtctgatcc
      661 gagcgtactt cgctccaatg cgggagcggg gctggggaag gatcatcaac ctcgggaccg
      721 tgggatccac ccgacccaac agccggatgc cccattacta cgcgtcaaag ggcgcgttgg
      781 ccaccatgac catcagcctt gccaaggagg catcgggcac gggagtgcgg gtgaatctgg
      841 tttctcccgg cctgatcctc acccccgagg tggaggccgc ctacctcgag cgcggccgag
      901 ccaagggctg gggagaaacc tgggaagagg tggagccaaa cgtggcggcc gacattccca
      961 tcgggcgcat cgtccgacgt gaggaggtgg ccgacctcgt cacgttcctg gcaagcccac
     1021 tggccgacgc cattcacggc cagaacatcc gggtggacgg cggggcgttg gacgtgctgt
     1081 cttgaccgga ccaggcctga ccagaccggg tatccggctg ctcgggctgg ccgcgctggg
     1141 cctgtgtgtg ctcgcctacg acgcgggcag ctacaccctc cttcaccgtc tcgtcctgcc
     1201 catcgccatg ggcgtggctg caggcctgat ggtgcagagt gcggccgccg ttctgctcgc
     1261 cggcaccctg ctcgccggcg ttcacgcccg tcctgcggac ccggactggg tcatagggat
     1321 cgcctatccg gcgctggccg ctctgtgtgg cgtggggctg acctggattg tcgtgctgcg
     1381 tttccgccgg cggatcgaag ccacgcgcga ggcacgctgg cgcggccgtc gcgagcgctc
     1441 ggcagcgccg gaaggatcgg agagctgaag tgggaacacg ctgcttcctg gtcagcggca
     1501 ccaccagcgg catcggtctg gccaccgcac gcgccctcgc ggccaccgga ggcaccgtcg
     1561 tcacggcaaa ccgcgaccct gacaggggcg agagcacggc cgccctgctg cgggatgcca
     1621 ccagcaaccc caacgtcatc aacaccgtgt gcaacctgtc ttcgttggca tctgtccgct
     1681 cctgcgcagc ccgcatccgg gagcagttcc cgcgcatcga cgtgctgatc aacaacgctg
     1741 gcgtgtggac cgccaagcct cagctttccg aggacggctt cgagctttct ttcgccacga
     1801 atcacctcgg gccgtttctg ctcacccaca tgctcctcga gccgctgaga gcagccggcg
     1861 gcgcaaggat cgtgaacgtc gcctcggtgc agcaccgcgc ggcggacctg gacctggatc
     1921 atctgacccc aaagacgccc tatcggggtc tgagggccta cagccggtca aagctcgcca
     1981 acgtgatgac gacgctcgcc tttgccaggc gcttcgacgc cgcggagatc tccgcaaact
     2041 gcctccatcc tggcgtggtt gcctccaaca tcgttcccga aaacagcccc ctgctgcgca
     2101 gagctgcgcg tgtcgtgagc ggcctgatgc gcagccccgc ccgcggggcc gcaacttcca
     2161 tctacctggc aacgtcgcca gaggtgacag gggtgacggg caaatacttc gaccccgacc
     2221 aacgcatcat ccaaccgtcc gcggaagccc gggacgaggc ggcacaggac ctgctctggg
     2281 aacgcagcct cgagctgacc gggctggcgg aggccggctg aaatgacacg aggcaaatcc
     2341 gccatggcgt ccgcggccga cggcatcgcc cgagcgattg ccatggggta cgaatcctat
     2401 caggcggagt ttcaggccat caccgccatg gcccaaagcc gtttcgaagg ccaggactgg
     2461 gctggcgttc aggacgcggg ggcgcgtcgc ctggccatct acggggagca cgtgcgcgac
     2521 gtggtcgctg aggtccatac gctcacggga gggtccgaga tcgacgcttc gctgtggcgc
     2581 aaatccaagc aggcgttcac atcgatcatc agcgagcact acaacgccga gctctacgag
     2641 acgttctaca actccattca tcgcgagatg accgacgaca gcgtagtcga cgacacggag
     2701 atgttcgttc gtaccctgtt ccccatcccg ccggtgcagc cagccgagcc ggtcttcgaa
     2761 agacacgcca tcgccggcga acccgaagag ttcgtgcgca cgctgctcga cggtctggcc
     2821 atcaaccggc catgggtcga ccgggaccgc gacgtgcgga acatactgcg ctctctttcc
     2881 gaggagcgcc cggagatcca ggacccgctc aggctggacg tggacatcct gaaacccatc
     2941 ttctacagaa acaagggcgc atacgtggtt ggccg
//
LOCUS       DBNHQR010000479         1859 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1313612, whole genome shotgun sequence.
ACCESSION   DBNHQR010000479 DBNHQR010000000
VERSION     DBNHQR010000479.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1313612"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..1139
                     /locus_tag="AC16VR_10835"
     CDS             51..1139
                     /locus_tag="AC16VR_10835"
                     /inference="COORDINATES: protein motif:HMM:TIGR03857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03857 family LLM class F420-dependent
                     oxidoreductase"
                     /protein_id="HIR6011696.1"
                     /translation="MSETPTSRFPELGFYGLPGHTRTPRDVLRQVQDAEALGIGNVMI
                     SERSDYKEIAAICGACAAVTESIYIGTSGTNLNTRHPVVTASMGSTLSRLSNGRFALG
                     LAKGSAMRWKAWGLPHPTYAREAEFVDLMRRLWRGETVSGYEGALGRYAHLALAPYVN
                     EDIPILYVGFGPKSLEHAGRIYDGAHLHTFMSDRALSEAVARIRSGEASAGRPAGTVK
                     AWSVLATACDVSEEVYLKYIIARLATYLQIPGYGDVLVAINGWDPEVLQRFRTHKAVT
                     SVGGLIDSVASTAQMAAVEKLIPESWRPAAVGDARTCAQRWVDQFNAGADGIIIHAST
                     PEEFEPVLREYEKIRPAERFAERTNRPG"
     gene            complement(1140..>1859)
                     /locus_tag="AC16VR_10840"
     CDS             complement(1140..>1859)
                     /locus_tag="AC16VR_10840"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR6011697.1"
                     /translation="LLEDLAPAVQGDQLAGCGAEVARSAVLELVGLQAPSWCREELKR
                     YDWLHAGDLSGAAMVGLYAQLLPGFLDRYGEALAADERRIIAKVAESPGCPLFQPVST
                     PFCLEHVDYRLDNMLIDARQTPPAVSVVDWQSVRIGKPMNDVAYFLGAGMLPDARRRV
                     EQDIVRDYHAKLTETGIEGYPWAQCWDDYRRGSFSGFGVTVVASMIVQQTERGDRMFI
                     TMARRHARHALDMGAEAFLSA"
BASE COUNT          331 a          650 c          601 g          277 t
ORIGIN      
        1 cgggccggat tgggtaacat ggtcgcgccc gatctgccca gagacgcatc atgtccgaga
       61 ccccgacttc ccgttttccc gaactgggct tctacggcct gcccggacac acccgcacgc
      121 ccagagacgt gcttcgccag gtgcaggatg cggaggcgct tggcatcggc aacgtgatga
      181 tctcggagcg gtccgattac aaggagattg ccgccatctg cggggcgtgc gcggcggtga
      241 ccgagtcgat ctacatcggc acctcgggca cgaacctgaa cacccggcac cccgtcgtga
      301 ccgcctccat gggcagcacc ctcagccgac tgtccaacgg ccggttcgca ctcggcctgg
      361 ccaaggggtc cgccatgcgc tggaaagcct gggggctacc gcacccgacc tacgcccggg
      421 aagcggagtt cgtcgacctg atgcggcggc tgtggcgcgg cgaaaccgtc tccggctacg
      481 aaggggcact cggcaggtac gcccacctgg cgctggcacc ctacgtgaac gaggacatcc
      541 ccatactcta cgtgggcttc gggccgaaga gcctcgaaca tgcgggccgc atctacgacg
      601 gcgcccacct gcatacgttc atgagcgatc gggccctgtc cgaggccgtc gccaggatcc
      661 ggtccggaga ggcgagcgcc gggagaccgg ccggcacggt gaaggcctgg tccgtactcg
      721 ccaccgcctg cgacgtgagc gaggaggtct atctcaagta catcatcgcc cggctcgcca
      781 cctacctgca gattccgggg tacggagatg tgctggtcgc catcaacggc tgggaccccg
      841 aggtcctgca acgcttccgc acccacaagg cggtgacgtc cgttggcggg ctgatcgaca
      901 gcgtggcaag caccgctcag atggcggccg tagagaagct catcccggaa tcctggcggc
      961 ccgcggcggt aggtgacgcc cgcacctgcg cccagcgctg ggtggatcag ttcaatgcgg
     1021 gcgcagacgg catcatcatc cacgcgagca cgccggaaga gttcgaaccg gtgctgcgcg
     1081 agtacgagaa gatacgccca gccgagcgct tcgcagaacg caccaaccgg ccgggctgat
     1141 caggcggaga gaaacgcctc ggcgcccata tcgagcgcgt gccgggcgtg ccgacgggcc
     1201 atggtgatga acatccggtc accccgctcg gtctgctgga cgatcatgga ggcaacgacg
     1261 gtcacgccaa aacccgagaa gctgccccgg cggtagtcgt cccagcactg ggcccagggg
     1321 taaccctcga tgccggtctc cgtcagcttc gcgtgataat cccggacgat gtcttgctcg
     1381 acccggcgcc gcgcgtcggg cagcatcccc gcaccgagaa aataggcgac gtcattcatc
     1441 ggtttgccga tccgcacgct ctgccagtcg accacgctca ccgccggggg cgtctgacgc
     1501 gcgtcgatca gcatgttgtc gagccggtaa tccacgtgtt ccaggcagaa gggtgtgctg
     1561 accggctgga acagcgggca gcccggcgac tctgccacct tcgcgatgat gcgccgttcg
     1621 tccgctgcca gtgcctctcc gtagcgatcc aggaagccgg ggagcagctg ggcatacagc
     1681 cccaccatcg ccgccccgga cagatcgccc gcgtgcagcc agtcgtagcg cttcagctct
     1741 tcccggcacc agctcggcgc ctgcagaccg accagctcga gcacggccga ccgggccact
     1801 tcggcaccgc atccggcaag ctgatcaccc tgcacggccg gcgcaaggtc ctcgagcag
//
LOCUS       DBNHQR010000480         5838 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1565607, whole genome shotgun sequence.
ACCESSION   DBNHQR010000480 DBNHQR010000000
VERSION     DBNHQR010000480.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5838)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5838)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5838
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1565607"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1374
                     /gene="mvk"
                     /locus_tag="AC16VR_10845"
     CDS             <1..1374
                     /gene="mvk"
                     /locus_tag="AC16VR_10845"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HIR6011698.1"
                     /translation="RAIEAAAHAYAARDGQYRSLTRWYKNEAGDLVGEIQIPMKVGTV
                     GGSLETNPSVRINHRLLGTPGATELAGIMAAVGLAQNFAALRALSTDGIQQNHMTLHA
                     RSVASTANVPEELFDTVVEALIESGEIKVWKAQEIVKNLTRKIAMPADVERSSACGKV
                     ILLGEHAVVYGRPALAVPIPLAVEASVRRGGGDGINLVIPRWGLEQKVRASNAQGLSG
                     ILYHVLEQLGLDQENMTIEVIPHVPRAMGLGGSSANAVAILRALDHTFRLRLSDGRLN
                     ELAYECETGAHGTASGIDNTIATYGAPLVFERGEDGPRFEEIRVAEPLPLVIGITGKE
                     CLTANTVALVRRAWEAHQARYDGIFDQIATLTRAGVDAMRDAHFAELGELMNLCQGYL
                     NALQLSTPELEELIHTARANGALGAKLTGGGGGGSMVALCPDSQDQVARAMEAAGYQA
                     LKVTIQP"
     gene            complement(1376..2074)
                     /locus_tag="AC16VR_10850"
     CDS             complement(1376..2074)
                     /locus_tag="AC16VR_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011699.1"
                     /translation="MDEAYRLVFRGEFLEGQHPAVARKRLVQALQLNDAQAEKLFSGH
                     PVVVKKRADAATAARYQDLFKQAGARLRVMPLASEAPAAEETTATDAGASSGASALEV
                     MPSGSDVLRADERSTFEELEIDLSHLAVLDWAPEVPDSAAPEIVAPDYSVADLGVDLA
                     ERISREHPGIKADFDLAEPGSLIPNLAVDTAPVVDVAAIEFDVAEVGADIGDPSDDAD
                     VTSPDVSHLSMAVE"
     gene            2321..3178
                     /locus_tag="AC16VR_10855"
     CDS             2321..3178
                     /locus_tag="AC16VR_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010562866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HIR6011700.1"
                     /translation="MSINIGKFEYPAHPKLTAHPTLWKVLPDARELVTANVERLSELQ
                     ERLYANNRYSVLMIFQGMDAAGKDSTIKYVTSGVNPMGFQVVNFKQPTYKELDHNYLW
                     RYWRAMPERGRIGIFNRSHYEEVGVARVHPEIIERQNLPSEHVNGDFWENRFEDIRAM
                     ERHLHTNGTIVLKFFLNVSKKEQKARLMKRLTNRDKHWKFDPRDIDERVHWDDHMKAY
                     EAAIAATHFDHAPWYVIPADDKWTMRVIVAEVLCTHLEQLRLSYPPAKGAHKHALAEA
                     LSRLEAEKE"
     gene            complement(3181..4341)
                     /locus_tag="AC16VR_10860"
     CDS             complement(3181..4341)
                     /locus_tag="AC16VR_10860"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR6011701.1"
                     /translation="MIEIHGYCDERFSPVREVFARNFADKDEVGASLALTVEGEMVVD
                     MWAGHRDAAKSLPWEQDTIVNVWSTTKTMSALCALVLADRGEIDLYAPVTRYWPAFGQ
                     NGKEKIEVRHFMSHSAGLAGTDEPIRGEALYDWERVVNALERQAPWWEPGTASGYHAI
                     TQGHLIGEVVRRVTGMSCGTFFRREIAEPLGADFHIGTAPEHFSRIGDLLPPGVSLGG
                     ALAHEPSGRGGGEGRAASIAVRAFRSPFVRAEDAATAEWREAEIPAANGHGNARSVAR
                     IQTVIANGGVSAGTRILSEAGCRAVFDEQTHGRDLVLKAPLTFGMGYALLHGQSPREL
                     AFWGGWGGSSVVVAPQSRMCIAYVMNRMEPSLLGDDRGVSLNRAALAVMGRG"
     gene            complement(4368..4811)
                     /locus_tag="AC16VR_10865"
     CDS             complement(4368..4811)
                     /locus_tag="AC16VR_10865"
                     /inference="COORDINATES: protein motif:HMM:NF013920.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HIR6011702.1"
                     /translation="MSYYLRPGLPQPAPLPDGLDRPYWDGLREDRLMLQRCAQCGGWQ
                     WGPEWICHHCLGFELAFEAVTPRGIVYSYERVWHPVHPALAEQGPYLVVLVEIPQADG
                     VRLVGNLLGDPLQDVEIGSAVEAVFEHHEDVDEPFTLLQWRLLAD"
     gene            complement(4808..>5838)
                     /locus_tag="AC16VR_10870"
     CDS             complement(4808..>5838)
                     /locus_tag="AC16VR_10870"
                     /inference="COORDINATES: protein motif:HMM:NF047238.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiolase C-terminal domain-containing protein"
                     /protein_id="HIR6011703.1"
                     /translation="VDGFASYSNDRSDPSRLAAALGCRELRFANMQWGGGGGGGSGAL
                     GNAAAAVATGMADCVVVFRALAQGQFGRFGQGPRRNVIAGDMDHVVPYGLMSPAQMFA
                     MKVQRFMHDHGVEQSALRAISLASYHHAQNNPRAVMHGRPLDEATYDASRWIVEPFHL
                     YDCCLENDGAAAMIVVPADRAGDCRPVPAYLLGAVSGSHYRAGASVHNTPDYATSTFK
                     TLAPRLYEMAGVVPADVDVLQSYENFTGGVLMSIVEHGFCEAQRCNEFFVPERLTAPD
                     GDLPLNTSGGNLAECYMHGLGLNIEAVRQIRGESTNQVMGAEISMVISGPMVTPVSAC
                     ILGSEAAL"
BASE COUNT         1125 a         1955 c         1830 g          928 t
ORIGIN      
        1 cgcgccatcg aagccgccgc tcacgcgtac gcggcccgcg atggccagta ccgctcgctg
       61 acacgctggt acaagaacga ggccggcgac ctcgtcgggg aaatccaaat ccccatgaag
      121 gtcgggaccg tgggcggctc gctggaaacc aaccccagcg tccgcatcaa tcaccggctc
      181 ctgggcaccc cgggggcgac ggagctcgcc ggcatcatgg ctgcagtagg gctcgcgcag
      241 aactttgccg ctctgcgcgc gctgtcgaca gatgggatac agcagaatca catgaccctg
      301 cacgcccgca gcgtggcgag cacggccaac gttcccgagg agctgttcga cacggtggtc
      361 gaagcgctca tcgagtcagg cgagatcaaa gtctggaagg ctcaagaaat cgtaaagaac
      421 ctgacccgga agatcgccat gcccgcagac gtggagcgaa gctcggcgtg cggcaaggtg
      481 atactgctgg gtgagcacgc cgtggtttac ggaagacccg ccctggcggt gccaatcccc
      541 ctggcggtag aggcctccgt gcgtcgcggc ggcggggacg gcatcaacct ggtcataccg
      601 cgctggggac tggagcagaa ggtccgcgcc agcaacgccc agggactcag cggcatcttg
      661 tatcacgttc tcgagcagct ggggctggac caggagaaca tgaccatcga ggtcattcct
      721 cacgtgcccc gcgccatggg gctgggtggc tcatcggcca acgccgtggc gatcctgcgc
      781 gcgctggacc acaccttccg gctcaggttg agcgacgggc gcctcaacga gctcgcctac
      841 gaatgcgaga cgggcgctca cggcacggcc tccggaatcg acaacaccat cgccacctac
      901 ggcgcgccgc tggtcttcga gcggggcgag gacggtccca ggttcgaaga gatccgcgtg
      961 gccgagccgc tgccgctggt catcggcatc accggaaagg agtgcctcac cgcaaacacc
     1021 gtcgccctgg tcaggcgggc gtgggaagcc caccaggcgc gctacgacgg gatcttcgac
     1081 cagatcgcca cgctgacccg cgccggcgta gacgccatgc gggacgctca tttcgccgag
     1141 ctcggcgagc tgatgaacct ttgccagggc tatctgaacg ccctgcagct ttcgaccccc
     1201 gagctggagg agctgatcca tacggcccgc gccaatggcg cgttgggagc caagctgacg
     1261 ggaggtggtg gcggcgggtc catggtcgcc ctgtgcccgg atagccagga tcaggtcgcg
     1321 cgcgccatgg aggctgccgg ctaccaggcg ctcaaggtga cgattcagcc gtagttcact
     1381 cgaccgccat actcaggtgt gagacgtccg gcgaagtgac gtcggcgtcg tccgacggat
     1441 cgccgatgtc cgcaccgacc tcggcaacgt cgaactcgat ggcggccaca tcgaccacgg
     1501 gcgcggtgtc caccgcaaga ttcggtatca ggcttccagg ctcggcaagg tcgaaatccg
     1561 ctttgatgcc gggatgctca cgggaaatgc gctctgccag gtcgacgccg agatctgcca
     1621 cggagtaatc cggggcgacg atttccggcg cggccgaatc gggcacctcg ggcgcccagt
     1681 cgagcaccgc caggtggctg agatcgatct cgagctcctc gaacgtggag cgttcgtcgg
     1741 cacgcagaac gtcgctgccg ctcggcatga cctccagcgc ggacgcaccg gagcttgcgc
     1801 ccgcatccgt cgccgtggtt tcttcggccg ccggtgcttc cgaggccagc ggcatcacgc
     1861 gcaatctggc gccggcctgt ttgaacaggt cctgatagcg tgctgcagta gccgcgtccg
     1921 cgcgcttctt gaccaccacc gggtgaccgc tgaagagctt ctccgcctgc gcgtcgttca
     1981 gctggagcgc ctgcaccagg cgtttcctgg ccaccgcggg gtgctgtcct tccaggaact
     2041 cgccgcggaa caccaatcgg taagcttcgt ccatcatgag cattctagaa ctgtgcgcca
     2101 catctacttc ggcgcaatga ccgcatgttc acacttctcg caagcggaaa atgggcacgg
     2161 gcgcacaagt cgcgccagtg attggtaaaa gcgtcccaga tatcggcgtt gagccgtggg
     2221 cacccgtgcg tttgttactc gacgtccctc atggcgctgg ttaaggtatc tcgaacggcg
     2281 caagcgggcg ccgcgcgcgc aaaggcacat tggactccag atgagcatca acatcgggaa
     2341 attcgagtat ccagcgcacc ccaagctgac agcgcacccc acgctgtgga aagtgctgcc
     2401 agacgcaagg gagctggtca ccgcgaacgt cgagcggctc tccgagctgc aggagcgact
     2461 gtacgccaac aaccgctatt cggttctcat gatcttccag ggcatggacg cagcggggaa
     2521 ggacagcacc atcaagtacg tcaccagcgg cgtcaatccc atgggctttc aagtggtcaa
     2581 cttcaagcag cccacctaca aggaactcga ccacaactac ctgtggcgct actggcgcgc
     2641 catgccggag cgaggccgca tcggcatctt caaccgttcg cactacgaag aggtcggagt
     2701 cgcccgcgtt caccccgaga tcatcgagcg gcagaatctg cccagcgagc atgtcaacgg
     2761 cgatttctgg gagaaccgct tcgaggatat ccgtgccatg gaacggcacc tgcacaccaa
     2821 cggcaccatc gtattgaagt tctttctgaa cgtttccaag aaagagcaga aagcgcgcct
     2881 gatgaagcgc ctgacgaacc gcgacaagca ctggaagttc gacccccgcg acatcgacga
     2941 gcgcgtgcac tgggacgacc acatgaaagc ctacgaggcc gccatcgcgg ccacccactt
     3001 cgatcacgcg ccctggtacg tgattccggc ggacgacaaa tggaccatgc gcgtcatcgt
     3061 cgccgaggtg ctctgcacgc acctcgagca gctcaggctc agctacccgc cggccaaggg
     3121 cgcgcacaag cacgcgctgg ccgaggcgct atcacgtctt gaagccgaga aggagtgagc
     3181 ctagccgcgg cccatgacgg caagcgccgc gcggttcaag gacaccccac gatcatcgcc
     3241 cagaaggctc ggttccatgc gattcatcac gtaagcgatg cacatgcgcg actgaggagc
     3301 gacgaccacc gaggacccgc cccagccgcc ccagaacgcg agttcccttg gagattgtcc
     3361 gtgtaacagg gcatatccca taccgaacgt gagcggcgct ttgagaacga ggtcgcggcc
     3421 gtgggtctgc tcgtcgaaaa ccgcccggca cccagcctcg gacaggatcc gagttccagc
     3481 agacacgccg ccattggcga tcaccgtctg gatgcgggcc acggatcgcg cgttgccgtg
     3541 accgttggcg gccgggatct ccgcttcgcg ccattccgcg gtcgccgcat cttccgcccg
     3601 cacgaacggg ctgcggaacg cgcgcaccgc gatgctggcc gctcggccct cgcctccacc
     3661 tctccctgag ggctcatgcg caagagcccc gccaaggctc acacccggcg gcagcaggtc
     3721 gccaatgcgc gagaagtgtt cgggagcggt gccgatgtga aagtcggcgc ccagaggctc
     3781 agctatctcg cgccggaaga acgtgccgca ggacataccc gtcacgcggc gcacgacctc
     3841 gccgatgagg tgtccctgag tgatcgcgtg gtatccgctc gccgttccgg gttcccacca
     3901 gggcgcctgc ctttcaagcg cgttgacgac ccgctcccag tcgtagagag cttcaccacg
     3961 aatcggctca tcggtgccgg caaggccggc ggagtggctc atgaagtgtc ttacctcgat
     4021 cttctctttg ccgttctggc cgaacgccgg ccagtaccgg gtgaccgggg cgtaaagatc
     4081 gatctcgccg cgatcggcga gcaccagggc gcaaagcgct gacatggtct tggtggtcga
     4141 ccacacgttg acgatggtgt cctgctccca gggcagagac tttgcggcat cccggtggcc
     4201 ggcccacatg tcgacgacca tctcgccctc aaccgtcagg gcgagcgaag ccccaacctc
     4261 gtccttgtcg gcaaagtttc gggcaaagac ctcacgcacg ggcgagaagc gctcgtcaca
     4321 gtagccgtgg atttcgatca tgctgaactc cgagttcaag ggcagaatta gtcggccaga
     4381 agcctccact gcagcagcgt gaagggttcg tccacgtcct cgtgatgctc gaacacggcc
     4441 tccacggccg agccgatctc cacatcctgg agcgggtcgc cgagcaggtt gccgaccaat
     4501 cgcaccccgt ccgcctgggg aatctcgacc agaacgacca ggtaagggcc ctgctcggca
     4561 agcgcgggat ggaccgggtg ccagacccgt tcgtagctgt agacgatacc gcgtggtgtt
     4621 accgcttcga aagcaagctc aaagccgaga cagtggtggc agatccactc gggaccccac
     4681 tgccagccgc cgcactgcgc gcaccgctgc agcatcagcc gatcctcgcg cagcccgtcc
     4741 cagtaaggtc ggtccaggcc gtccggaagc ggcgcgggct gtggcaggcc gggtctgagg
     4801 tagtagctca tagcgccgcc tccgaaccca ggatgcacgc gctcaccggg gtgaccatgg
     4861 ggcccgagat caccatggat atctctgcgc ccatcacctg attggtggac tcaccgcgaa
     4921 tctgcctaac cgcttcgatg ttgagcccca gaccgtgcat gtagcactcg gcgagatttc
     4981 cgccgctggt attcagcgga aggtccccat cgggcgccgt caaccgctcc gggacgaaga
     5041 actcgttgca ccgttgcgcc tcgcagaatc cgtgctcgac gatgctcatg agcacgccgc
     5101 cagtgaagtt ctcgtagctc tgcagcacgt cgacgtcggc ggggaccacc ccggccatct
     5161 cgtacaggcg gggagccagc gtcttgaacg tgctcgtcgc gtaatcgggc gtgttgtgca
     5221 ccgaagcccc ggcccgatag tgggaaccgg acacggcacc aagcagatag gcaggaaccg
     5281 ggcggcagtc gcctgcacga tccgccggca ccacgatcat ggcagccgcg ccgtcgttct
     5341 cgagacagca atcgtagaga tgaaacggct cgacgatcca tcgagacgca tcgtaagtcg
     5401 cctcatccag aggccgtccg tgcatgaccg cacgggggtt gttctgagca tggtggtaag
     5461 acgccaagga gatcgcccgc agcgccgact gctcaacacc gtggtcgtgc atgaatcgct
     5521 gcaccttcat cgcgaacatc tgagctggcg acatcaggcc gtacggcacc acgtggtcca
     5581 tgtcaccggc gatgacgttg cgtcgcgggc cctgcccgaa ccggccgaac tgcccctggg
     5641 cgagcgcgcg aaaaaccacc acgcaatcgg ccatgccagt ggcaacggcg gccgcggcgt
     5701 tgccgagcgc cccggagccg ccccctccgc caccgcccca ctgcatgttg gcaaagcgga
     5761 gttcacgaca acccagcgct gcggccagcc gcgacgggtc gctgcggtcg ttgctgtagg
     5821 aagcgaaacc atcgacgt
//
LOCUS       DBNHQR010000481         3911 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1313950, whole genome shotgun sequence.
ACCESSION   DBNHQR010000481 DBNHQR010000000
VERSION     DBNHQR010000481.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1313950"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="AC16VR_10875"
     CDS             complement(<1..420)
                     /locus_tag="AC16VR_10875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HIR6011704.1"
                     /translation="MTQRIEIHAELREDVGKGASRRLRRLADKVPGVIYGGDADPVPL
                     MLSSNELSKAMQLEAFYSQILNVVLDGKGEQAVVRDLQRHPASEKVQHIDFLRIRADR
                     LIQVSVPLHFIDEDKCIGVKQGGGQIVHNMNEVEISCL"
     gene            complement(490..1434)
                     /locus_tag="AC16VR_10880"
     CDS             complement(490..1434)
                     /locus_tag="AC16VR_10880"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HIR6011705.1"
                     /translation="MSNLMIFTGSSNPVLSARIAEELRMDLGHADLGRFSDGEVNVEI
                     HENVRGQDVFVIQSTCAPTNDNLMELMIMSDAIRRASAQRVTAVIPYFGYARQDRRAR
                     SARVPISAKVVADMLGSVGIDRMLTVDLHADQIQGFFNMPVDNVYGSPVLIDHMLGEH
                     YENPIIVSPDVGGVVRARAVAKQCNDLDLAIIDKRRPQANDSRVMNVIGDVRGKTCIL
                     VDDIVDTAGTLCTAAEALKEEGAIGVVSYITHAVLSGPAIERIAGSELDRMVVTDTIP
                     LSGSAESCSKIEQLSLARLLAEAIRRVSNEESISAMFR"
     gene            complement(1501..1575)
                     /locus_tag="AC16VR_10885"
     tRNA            complement(1501..1575)
                     /locus_tag="AC16VR_10885"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1541..1543),aa:Gln,seq:ttg)
     gene            complement(1725..2255)
                     /gene="lolB"
                     /locus_tag="AC16VR_10890"
     CDS             complement(1725..2255)
                     /gene="lolB"
                     /locus_tag="AC16VR_10890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HIR6011706.1"
                     /translation="MALCAGCASFRPPVETPADFEVRGKAGVVEGAESFSARFVWRQY
                     GSAFTIDLWGPLGQGRVRLDGDERRLAIVDGKGAVISEGPHAEVMRTHLGWSLPLDVL
                     PSWVLGKPGEGTGAGGRQYDDDGRLVAFEQLDWSVAYARHNQVQTARGSRWLPGRITA
                     VRGDYRVRLSISDWVL"
     gene            2364..3617
                     /gene="hemA"
                     /locus_tag="AC16VR_10895"
     CDS             2364..3617
                     /gene="hemA"
                     /locus_tag="AC16VR_10895"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HIR6011707.1"
                     /translation="MAILAYGINYRTAPIDVRERVAFPEDALPAALEDAMSSIPDVSE
                     IAIISTCNRTELIVSAAPEAEESVARWLASYRPVSMGELNSMAYSHWDQDAARHLIRV
                     AAGLDSQVLGEPQIMGQVKQAYELARAAGALGSELSLLSRLTLNAAKRIRTDTDIGRN
                     PVSVAYAAVTMAQQIFADLHHNKALLIGAGDTIQRVAEHLSALGTAGLAIANRTLANA
                     EALAAGFDAVPMQLTDIAAHLADYDIVITSTGSSLPVLGKGTVEAAIKRRRHKPMFIV
                     DIAVPRDVEPEVAELPDVYLYTIDDLTEIIEENIKQRRSAAESAEHLVMEGAEAYTRE
                     RRAHEGQALLRSFRENAEAIQRAELEKALTALGRGGDPNEVLKSLSRSLTNKLIHQPT
                     VAIRDASASDRSDLLEYLKSLYQLD"
     gene            3621..>3911
                     /locus_tag="AC16VR_10900"
     CDS             3621..>3911
                     /locus_tag="AC16VR_10900"
                     /inference="COORDINATES: protein motif:HMM:NF015427.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HIR6011708.1"
                     /translation="MSLSASMMAKLSDLTDRFDEVSALLSDPETMADRDRFTALSKEY
                     AELEPLILTFRRHDVLDTELREAQELAGDEDPEMRELAREDVDRLSKALDEEE"
BASE COUNT          740 a         1287 c         1193 g          691 t
ORIGIN      
        1 cagacagctg atctcgacct cgttcatgtt atgcacgatc tgaccgccgc cctgcttcac
       61 gccgatgcac ttgtcctcgt cgatgaagtg gagcggcacg gacacctgaa tgagacggtc
      121 ggcgcggatt cgcagaaagt cgatgtgctg caccttttcc gaggccggat ggcgttgcag
      181 gtcccgtacc accgcctgct caccctttcc gtccagaacg acgttcagga tctgcgagta
      241 aaaggcctcg agctgcatgg ccttggaaag ctcgttgctg ctcagcatca gcgggaccgg
      301 atcagcatca cccccataga ttacgccggg cactttgtct gcaaggcgac gcaggcggcg
      361 gctcgcacct ttccccacgt cctcgcgcag ctcggcatga atttcaattc gctgcgtcat
      421 cagatttctc catcaaatac ggacgtcccg gatcgcgacc aagtcctggc gtccaattgc
      481 aaccatcagc tagcgaaaca tcgcgctgat ggattcctcg ttactcaccc gtcggatggc
      541 ttccgccaga agacgggcga ggctgagctg ctcgatcttc gagcagcttt cggccgatcc
      601 gctcaacggt atcgtgtcag tcacgaccat ccggtcgagc tcggagccgg cgattctttc
      661 gatggcgggt cccgaaagaa ccgcatgcgt aatgtaggaa accacgccga tggcgccctc
      721 ttccttgagc gcttcggccg ccgtgcacag cgtgcccgcc gtatcgacga tgtcgtcaac
      781 caggatgcac gtcttgccgc ggacgtcgcc gatgacgttc atcacgcggg aatcgttcgc
      841 ctgcgggcga cgtttgtcga tgatggcgag gtccagatcg ttgcactgct tggccaccgc
      901 gcgagcgcgc accaccccgc ccacgtcggg tgagacgatg atgggattct cgtagtgctc
      961 accgagcata tggtcgatca ggaccgggga cccgtaaacg ttgtccaccg gcatgttgaa
     1021 aaagccctgg atctggtccg cgtggagatc gacggtgagc atacggtcga tgccgacgct
     1081 gccgagcatg tcggcgacca ccttggcgct gatgggcact cgtgccgagc gggctcgcct
     1141 gtcctgacgc gcatagccaa aatacggtat caccgccgtc acgcgctgcg ccgatgcgcg
     1201 gcggatggcg tcggacatga tcatgagctc catgaggttg tcattggtcg gagcgcaggt
     1261 ggattggatg acgaagacgt cctgaccgcg cacgttctcg tggatctcca cattcacctc
     1321 accgtcgctg aacctcccca ggtcggcgtg cccgagatcc atccgcagct cctcggcgat
     1381 gcgggcgctc agcacggggt tcgagctccc cgtgaagatc atcaggtttg acaattggct
     1441 tcccctcgtc cgcgctcttt gcgcgtgcta tgcggttgcg gcttcaagag atcaacagtt
     1501 tggctggggt ggcaggattc gaacctgcga atgccgggat caaaacccgg tgccttacca
     1561 cttggcgaca ccccaacaga cagctcgttg ccatgtgcgc ccgccggcgc tgttcctgac
     1621 gtttcgcggt tctcgccccg gcagcggggc gctattgtga tgttgggtct gttggctgtc
     1681 aaaccgccag acctgacggg cagacgtcca cgtatcgggg cccgtcagag cacccagtcg
     1741 gatatggaaa gccgcacccg atagtcgccc cgcacagccg tgatccggcc cggcagccag
     1801 cgggaaccac gagccgtctg cacctggttg tgccgggcgt aggcgactga ccagtcgagc
     1861 tgctcgaaag cgaccaggcg cccatcatcg tcatactgcc ggccgccggc gccggttccc
     1921 tctcccggct tgcccagcac ccagctgggc agcacgtcca gtggaaggct ccagccaaga
     1981 tgcgtacgca tgacctcggc gtgaggcccc tcggagatca cggctccctt gccgtctaca
     2041 atggccaggc ggcgctcgtc cccatcgagc ctgacgcgcc cctgcccgag gggcccccag
     2101 aggtcgatgg tgaatgccga gccgtactgc ctccagacga agcgcgccga aaaactctcc
     2161 gcgccttcga cgacgccggc cttgccgcgc acctcgaagt cggccggggt ctcgacggga
     2221 gggcggaacg acgcgcagcc ggcgcagagg gccaaacaaa acaactttaa aatgaatggc
     2281 ttaagtacgt tcacaaatct ccgaagctgc tagaatgctg ggtcggcaag aaggctctca
     2341 gctgagatca taccctgcac aacatggcga tattggcata cggcattaac tatcgcacgg
     2401 ctcccatcga cgtccgggag cgcgtcgcat ttccggagga cgccctgccg gcggccctag
     2461 aggatgccat gtcgtctatt cccgatgtca gcgagatcgc catcatctca acgtgcaacc
     2521 gcacggagct catcgtctcc gcagcgcccg aggccgagga atccgtggcc cgttggctgg
     2581 cgagctatcg gccagtgagc atgggcgagc tgaacagcat ggcctacagc cactgggacc
     2641 aggacgcagc gcgacacctg attcgtgtcg cggcgggcct ggattctcag gtgctcggcg
     2701 agccgcagat catgggccag gtgaaacagg cttacgagct ggctagggcg gccggcgctc
     2761 ttggttccga gctgagcctg ctgtcccggc ttacgctcaa tgccgccaag cggatccgca
     2821 ccgacaccga tatcggccgc aaccccgtct cggtggccta cgctgcggtc accatggcgc
     2881 agcagatctt tgccgacctg caccacaaca aagcactgct catcggcgcc ggcgacacga
     2941 ttcagcgggt ggccgagcac ctttccgcgc tgggcaccgc gggcctggcc atcgcgaacc
     3001 ggaccctggc gaacgcggag gctcttgcgg caggctttga tgccgtcccc atgcagctga
     3061 ccgacatcgc cgctcacctt gccgactacg acatcgtcat cacgtccacc ggcagctcac
     3121 tgcccgttct cggcaaaggg accgtggagg ccgccatcaa gcgtcggcgc cacaagccga
     3181 tgttcatcgt cgacatcgcc gtgccgcgag acgtggagcc tgaggtggcc gagctgcccg
     3241 acgtgtacct ctacaccatc gacgatctca ccgagatcat cgaggagaac atcaaacagc
     3301 gccgatcggc tgccgagagc gccgagcatc tggtcatgga aggcgccgaa gcctacaccc
     3361 gcgagcgtcg cgcccacgag ggccaagcgc tgctgcgcag tttccgcgag aacgccgaag
     3421 ccatccaacg ggcggagctc gaaaaagcgc tgacggcact gggccggggg ggcgacccga
     3481 atgaggtgct gaagtcattg agccggtctc tgaccaacaa gctgattcac cagccgaccg
     3541 tcgcgattcg cgatgccagc gcgagtgacc gcagcgatct cttagaatat ctcaagtctc
     3601 tctaccagct agactgaatc atgagcctgt ccgcctcgat gatggccaag ctgtcagatc
     3661 tgaccgaccg cttcgacgag gtcagcgccc tcctgtcgga tcccgagacg atggccgacc
     3721 gggaccggtt taccgcgttg tccaaggagt acgccgagct ggagcccctc atcctgacgt
     3781 tccgccgcca cgacgtgctg gacacagagc tgcgcgaagc ccaggagctc gccggggatg
     3841 aggaccccga gatgcgcgag ctcgcgcggg aggacgtgga tcgcctgtca aaggcgctcg
     3901 acgaggagga g
//
LOCUS       DBNHQR010000482         3676 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1439198, whole genome shotgun sequence.
ACCESSION   DBNHQR010000482 DBNHQR010000000
VERSION     DBNHQR010000482.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1439198"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            363..713
                     /locus_tag="AC16VR_10905"
     CDS             363..713
                     /locus_tag="AC16VR_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011709.1"
                     /translation="MAHDDAQHCGDCPDGSPSSPMQGTDSHGDVAAGHQGDSGCGFDC
                     SPDGSTDTGHAGCGFDCAMLSSGHCGSPVSPTLAAMALLATTSLADAFGRDRSSVMLP
                     DPPLFDFLRPPTRS"
     gene            787..1299
                     /locus_tag="AC16VR_10910"
     CDS             787..1299
                     /locus_tag="AC16VR_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011710.1"
                     /translation="MNTLKIIALVPAAFVAVAAAPYAGAAAKPTAGHDHAAMVQDHQA
                     KSATSTNTVAAHEAMANHCEGMMDHAEAAATGHSDTAGRHPCRAEAQSATLTFEQHLA
                     AARELEAKAAAASAKASEHEKMARAGGAPKDDGRSMDNHCSELVIRYRAQAESYSAQA
                     AEHKRLAAAR"
     gene            1534..2583
                     /locus_tag="AC16VR_10915"
     CDS             1534..2583
                     /locus_tag="AC16VR_10915"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR6011711.1"
                     /translation="MVAHSIAIALGGGGARGLAHLGVLEVLDDQGIRPAFLSGTSMGG
                     LIAALRATGTDPDDITATARRFKLPSRFLPGRLLVWDTIFASAVPRLEGRTFEDLATP
                     LVVSAVDLMTGEEVALCSGSLLPAVRATCAVPGVFPPEKVGARCLVDGGVTNMLPVDL
                     AWACDPDIVVAVNIVASPPSTLRLDSAYCRAATRLGKVVANPLTALFSYEVAMRAAEI
                     ALDRQRAMAVAMTGPEVLIDVDLSHVAIDDFQRFDEIVEAGRNATRRALPGIQAALSA
                     PADGGERATADATLHIDPVCRMTVSSARARATGEREGKTFYFCSTSCRDIFARHGDRY
                     VGATALRPPIVRGKS"
     gene            complement(3029..>3676)
                     /locus_tag="AC16VR_10920"
     CDS             complement(3029..>3676)
                     /locus_tag="AC16VR_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011712.1"
                     /translation="TLLVAAPSVADKLEAVHMVDFPSSARAKANIDALWMDPAMQGRK
                     LTLYAQEFGDLQASHLVVEDFDSYEEYMDSTAKRFASPGWARYALDSDVFEYYRGSNL
                     VTVVDDHGVKRRSAGYLAAYLVNVTDAAAYRAGIADLNRAVGNPGVLRLVAFRSGNMG
                     VTHAVLIGGPDFKAVNEYLDKLFASEAFAKFTSKVGPIRKLVSVQMYRRVGAWGD"
BASE COUNT          616 a         1225 c         1197 g          638 t
ORIGIN      
        1 gatttcggac tttgaggtgt cgactcgaat cggcagcgcg gcctgtccgc tatgggtcgg
       61 atgcggccat gcttggcctg accccccgtc ttccgcgagg ccaggcgtcc gtcggttctg
      121 cgatctccag ggcgccctct cctccgttta cgatccaccg gacggcgcca gcgaaccggg
      181 ccgtccgggc gccttgatga ccggaccgtc ggcaaaccac gacacggcta cgtgtacctg
      241 tgtatgtctc gcagcggcaa aaggctcaga ctcattgcat gtgcggtcgg tctcattccg
      301 tcctgttcgc agcgctgctc ctcgtggtac agctgccgct cggagtgttg tcaggcggag
      361 ccatggcgca cgacgacgcg cagcattgcg gcgactgccc cgacggcagt ccgagcagcc
      421 cgatgcaggg aacggactcc catggtgacg tggcagcagg tcaccaaggt gattcgggct
      481 gcggcttcga ctgttcgccc gatggctcga ccgataccgg tcatgcgggc tgcgggttcg
      541 actgcgccat gttgagcagc ggtcactgcg gctctcccgt cagcccaaca ctcgccgcca
      601 tggcgctgct ggccacgacg tctctcgccg acgcattcgg ccgcgaccgt tcgtcggtca
      661 tgctgccaga cccaccgctc ttcgactttc tccgtccgcc gactcgcagc tgaatcagct
      721 gcactcggtt tcccatcgcc tggcgaggcc caggcgacct gatgacttct ttcgacggag
      781 attgaaatga acaccctcaa gatcattgcc cttgtcccgg ccgcgttcgt tgccgttgcc
      841 gcggctccct acgccggcgc cgcagcgaaa cccacggcag gccatgacca tgctgcgatg
      901 gtgcaggacc accaggccaa gtccgccacg tcgacaaata cggtggctgc gcacgaggcg
      961 atggcgaacc attgtgaagg catgatggac catgcagagg cggcggctac cgggcattcg
     1021 gacacggctg gccggcatcc atgccgcgca gaggcccagt cggcgacact cacgttcgag
     1081 cagcacctcg ccgccgctcg cgagctcgaa gccaaggcgg cagctgccag cgccaaagcg
     1141 tccgagcacg agaagatggc tcgcgctggt ggcgcgccga aggacgacgg gcggtcgatg
     1201 gacaatcact gcagtgagct ggtgatccgg taccgtgcac aggccgagtc gtacagcgcg
     1261 caggccgcgg agcacaagcg gctcgcagct gccaggtagt tgcgtgacgg gaggctacgg
     1321 gcgaccgtag cctcccccgc tgtcggcggg cacatcgcgc gcgtcgagca tccggtcccg
     1381 gtcgtcagcg aacttctgga tccgggatgc gccaggcctc gcgccgtcat cgtcgacgcc
     1441 agacggcgct ccctgcgaca caattgccgt cggcacgacc tacgtcacga agtccgtatt
     1501 gaaagcgagg cgacccagcg tagtctgacc aagatggttg cacattccat cgccatagcg
     1561 cttggcggcg gcggcgcccg gggcctcgcc cacctggggg tgctcgaggt gctcgacgac
     1621 cagggtatcc ggccggcgtt cctctcaggt acgagcatgg gcgggctgat cgccgcgctg
     1681 cgtgccaccg ggaccgaccc ggacgacatc acggccacgg cccggaggtt caagctgcca
     1741 agccgcttcc tgccggggcg gctgctcgtc tgggacacga tcttcgcctc cgcagtgcca
     1801 cggctggagg ggcggacatt cgaggacctg gcgaccccgc tcgtggtgtc ggcggtggac
     1861 ctgatgaccg gcgaagaggt ggcactctgc agcgggtcac tgctccctgc ggtcagggcc
     1921 acctgcgcgg ttcctggcgt gtttccgccc gagaaggttg gcgcccgctg cctcgtcgac
     1981 ggcggcgtga ccaacatgct gcccgtggat ctcgcctggg cgtgcgaccc tgacatcgtc
     2041 gtcgccgtga atatcgtcgc ttcgccacct tcgaccctgc gtctcgactc ggcttactgc
     2101 cgggcggcaa cgaggcttgg caaggtggtg gcaaatccac tcaccgccct gttttcctac
     2161 gaggtcgcga tgcgtgccgc cgagatcgcc ctggaccggc aacgggccat ggcagtcgcg
     2221 atgaccggac cggaggtcct gatcgacgtc gacctcagcc acgtagccat cgacgacttc
     2281 cagcggttcg acgagattgt cgaggccggg cggaatgcca cgcggcgggc gcttccggga
     2341 atccaggctg ccctttcggc gccggccgat ggaggcgagc gggccaccgc cgatgccacg
     2401 ctgcacatcg acccggtgtg ccgcatgacc gtgagctcgg cgcgggcacg ggccacgggt
     2461 gagcgtgaag gcaagacgtt ctacttctgc tccaccagct gccgcgacat attcgcgcgc
     2521 cacggtgacc gatatgtcgg ggcaacggcc ctgcgcccac cgatcgtgcg cggcaagagc
     2581 taggccaccg gagtgccgga agccggccgc gcccggcctc gacctgctgg cgcccatgga
     2641 gttcgggtgc gaaccgtccc gcgagatggc gttgggtggg gctgaaagga caaggggctc
     2701 acgcggtttc gtaaaccctc ggtctggatg gcgcgtgcgg agaggttact cgcggcactg
     2761 cgtggagcct cgccgaacat cctgaaccga aggttcgtag gcggtcctga gagacagcag
     2821 aactttcttg gccaacagcc tctagcgctt tagggtcctg aaaccagcct gtccccggca
     2881 cgggcagtct caaggcgtcg cctctgcggc gcatgtccga tgacggccag aagcggacaa
     2941 gggcctggga agggaaaccc cggcgcgggg ccggggttcc gtagcttgca acgatcagac
     3001 tgctcgcggc agttgaggtc gaacgtgttc aatctcccca ggcacccacg cgccggtaca
     3061 tctggacgct gaccaactta cgtatcgggc cgaccttgga agtgaacttt gcgaatgcct
     3121 cgctcgcgaa cagcttgtcg aggtactcgt tgaccgcctt gaagtcgggc ccaccgatta
     3181 gtactgcgtg tgtcacaccc atgttgccgc tgcgaaacgc aaccagccgc aggacgcccg
     3241 ggttgcctac tgcacgatta aggtcggcta ttcccgcccg ataagccgcg gcatcggtga
     3301 cgttgacgag gtaggctgct aagtagccgg cactgcgccg cttcacgcca tgatcatcta
     3361 ccaccgtcac cagattgctt ccgcgatagt actcgaagac gtctgaatca agcgcgtacc
     3421 tggcccagcc gggactggcg aaccgtttcg ccgtggagtc catgtactcc tcgtagctgt
     3481 cgaaatcctc gacgacgaga tgcgaggcct gcagatcccc gaattcctgc gcgtaaaggg
     3541 tcagcttgcg accctgcatt gcaggatcca tccacagcgc gtcgatattt gccttggcgc
     3601 gcgcgctgct cgggaagtcg accatgtgga ctgcctcgag cttgtcggct accgatggtg
     3661 cggccaccag cagcgt
//
LOCUS       DBNHQR010000483         3357 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1185349, whole genome shotgun sequence.
ACCESSION   DBNHQR010000483 DBNHQR010000000
VERSION     DBNHQR010000483.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1185349"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="AC16VR_10925"
     CDS             <1..202
                     /locus_tag="AC16VR_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011713.1"
                     /translation="PIITFSEIPEIKAHLIDRRHEPALGAGEASTGPTPAAIANAIGN
                     AAGVRVRDIPFTAEALRRAAAE"
     gene            286..1005
                     /locus_tag="AC16VR_10930"
     CDS             286..1005
                     /locus_tag="AC16VR_10930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR6011714.1"
                     /translation="MSADATPAAIGLLEGIATTRAIRRYLDEPIPDEALRDILFAATR
                     GPSGSNRQPFRFVVLTDSPKAKEAKRLIGSAARSMWSDKRRNDGYDQGSGADESSPKA
                     RMARTMQSYVDEFDRVPVLVLPCLVRYRAPTPMEGASVYPAVQNLLLAARALGYGGVI
                     TGFHGAVDAQLKALLGIPEEVFIAATVTLGRPRGAHGPVRRRPMRELVYGDEWGTPPA
                     WAEDPPGTRYTSAGPPKRKPT"
     gene            1022..2656
                     /locus_tag="AC16VR_10935"
     CDS             1022..2656
                     /locus_tag="AC16VR_10935"
                     /inference="COORDINATES: protein motif:HMM:NF013086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6011715.1"
                     /translation="MEADVIVLGTGAAGLTAALAAHGHGADVLVLEKSDKVGGTSAWS
                     GGMIWIPCNHHMRAAGIEDSREEALTYLSALSHDLIDPDLAEAFVDTGPEMVAWMESN
                     TPVQFQLVPDFPDYHPEHPGGKPGGGRSLECPLFAYETLEAHRDLVTVGYNYGTAPIT
                     MEESNLGRAVPIEVPEEERARRAERDERGCGQALMGRLFKGCLERNITVRTGFRAMRL
                     LVEDGSVKGVAGEGPDGPFEFSARGGVVLATGGFEWNHDLVRTFLRGPMTSPVSVPTN
                     EGDGLTMSMRIGAALGNMREAWWMPAVEIPGDRGDGRPHQHLFASERARPRSIMVNRA
                     GRRFTNEAANYNAFGAAFHEQDVARFDYANLPCWFIFDQKHLDTYGFIGTPPGAPAAN
                     WVANAPTLRALAAQLGIDADGLDKTVAQWNGNVAEGRDPDFHRGESAHDTWWGDPWEK
                     GTPAATLGPVDTGPFYAVEVRSGALGTKGGPRTDGLARVKNVDGEIISGLYAAGNVMA
                     SAMGMTYGGAGGTLAPGMVFGFLAGRDAARRAVGKS"
     gene            complement(2683..3183)
                     /locus_tag="AC16VR_10940"
     CDS             complement(2683..3183)
                     /locus_tag="AC16VR_10940"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6011716.1"
                     /translation="MGTVNRVNHTGISVADMERSLAFYRDLLGLELIFDSDVDDVVDL
                     NAVVGMERARGRVAWLRAGDTMIELWQWDHPRGRPLPKDYRPADRGVTHFALEVDDVD
                     QLCERIVDAGYHANTTPKDLGLHKTTYVRGPDDEIIEILEDRATDEWLARLTAEAEAR
                     RREGDA"
BASE COUNT          607 a         1159 c         1074 g          517 t
ORIGIN      
        1 ccccatcatc actttcagcg agattccgga aatcaaggcc catctcatcg accggcgcca
       61 cgaacccgcg ctgggagccg gagaagccag caccggcccg acgccggcgg cgatcgccaa
      121 cgcgataggc aacgccgccg gcgtaagggt ccgggacata ccgttcaccg ccgaggcctt
      181 gcgacgcgcg gccgccgagt agcgctggga cgccgtgagc tactatgttc cccatctctt
      241 ctacaccata atcgagtgcc gccgcacgtc catcaggaac tcacaatgtc cgccgacgcc
      301 acgcccgccg ccatcggcct gctggaaggt atcgccacca cgagggccat ccgccggtac
      361 ttagacgagc ccattcccga cgaagcgctg cgcgacatcc tgtttgccgc cactcgcggg
      421 ccgagcggct ccaaccggca gccgttccgc ttcgtggttc tgaccgacag ccccaaggcg
      481 aaagaagcca aacgcctgat cggctctgcg gcgcgaagca tgtggtcgga caagcgccgc
      541 aacgacggtt acgaccaggg ctcgggcgcc gacgagagct ccccgaaagc gcgcatggcc
      601 cgcaccatgc agtcctacgt ggacgagttc gaccgggttc ccgtgctggt gctaccctgc
      661 ctggtacgct accgggcgcc cacgcccatg gaaggcgctt cggtgtaccc cgctgtgcag
      721 aacctgctgc ttgcagccag ggcactgggc tacggtggcg tcatcacggg cttccatggc
      781 gccgtggacg cgcagctgaa ggccctgctt ggtattcctg aagaggtgtt catcgccgcc
      841 accgtgaccc tgggtagacc acggggtgcc cacggtcccg tccggcgaag acccatgcgc
      901 gagctcgtct acggggatga gtggggcaca ccgcccgcct gggccgagga ccctccggga
      961 acccgttaca ccagcgcggg ccccccaaag cgaaaaccca cctgaccgag tggaggtatc
     1021 gatggaagca gacgtcatcg tgctgggcac cggagccgcc ggcctgaccg ccgctctagc
     1081 ggcacacggc cacggggcag acgttctggt gctcgaaaaa tcggacaagg tcggcgggac
     1141 cagcgcgtgg tccggcggca tgatctggat cccctgcaac catcacatgc gcgctgccgg
     1201 catcgaagac tccagagaag aagcgctcac gtatctatcg gccctgtctc acgatctcat
     1261 cgaccccgac ctcgccgagg ccttcgtgga cacaggcccg gaaatggtcg cctggatgga
     1321 gtccaacact cccgttcagt ttcagctggt accggacttt cccgactacc acccggagca
     1381 ccccggcgga aagccgggcg gcggacgttc cctggagtgc ccgctgtttg cctacgaaac
     1441 gctcgaagcg caccgcgatc tggtcaccgt gggctacaac tacggcaccg cgcccatcac
     1501 gatggaagaa tccaacctgg gtcgcgcggt gcccatcgag gtcccagagg aagagcgggc
     1561 ccgccgggcc gagcgcgacg agcggggttg cggccaggcg ctgatgggac gactgttcaa
     1621 ggggtgcctc gagcggaaca tcacggtaag aacggggttc cgggccatgc gcctgctggt
     1681 ggaggacggc tcggtgaagg gcgtcgcggg cgaaggcccc gacggcccgt ttgagttttc
     1741 cgcccgcggc ggcgtggtgc tggcgacggg cggtttcgag tggaaccacg acctggtccg
     1801 gacttttctg cgcggaccga tgaccagccc ggtatccgtg cctaccaacg agggtgacgg
     1861 gttgaccatg agcatgcgca tcggcgctgc gctgggcaac atgcgcgagg cctggtggat
     1921 gcccgcggtg gagatccccg gtgaccgcgg cgacggccgc ccgcaccagc acctgttcgc
     1981 atccgagcgc gcgcggccca ggtcgataat ggtcaatcgg gccggccgcc gtttcaccaa
     2041 cgaggcggcc aactacaacg cgttcggggc cgcctttcac gagcaggacg tggcgcgctt
     2101 cgactacgcc aacctgccct gctggttcat cttcgaccag aaacatctgg acacctacgg
     2161 cttcatcggc accccgccgg gggcgcccgc cgccaactgg gtagccaacg cgcccactct
     2221 gcgagcgctg gctgcccagc tcggcatcga tgccgacggg ctggacaaga cggtggcaca
     2281 gtggaacggc aacgtggcgg aaggccgcga tccggacttc caccgcggcg agagtgccca
     2341 cgacacctgg tggggcgatc cctgggagaa aggcacgccg gccgcgacgc tgggccctgt
     2401 tgacaccggc ccattttacg ccgtggaggt caggagcggc gcgctgggca ccaagggcgg
     2461 cccgagaacc gacggcctcg ccagggtgaa aaacgtggac ggcgaaatca tcagcgggct
     2521 gtacgccgcc ggcaacgtca tggcctcggc catgggcatg acctacggag gcgccggcgg
     2581 aacgctggcg cccggcatgg tcttcggatt tctcgcggga cgcgatgcag caaggcgggc
     2641 tgtcggaaag agctgacgcc ccggtcaggg cggacattcg gcctacgcgt caccctcgcg
     2701 acgccttgcc tcggcctcgg ccgtcagccg cgccagccac tcatcggtag cacgatcttc
     2761 gagtatctcg atgatctcat cgtccggccc ccgcacgtac gtcgtcttgt gcaggcccag
     2821 atctttcggc gtggtattgg cgtgatatcc ggcatccact atgcgctcgc agagctgatc
     2881 gacgtcgtcc acctccagcg cgaaatgggt cacgcctctg tccgccggcc ggtagtcttt
     2941 gggcagcggc cggcctcgcg ggtgatccca ttgccacagc tcgatcatgg tgtcgcccgc
     3001 gcgcagccag gccaccctgc cccgggcgcg ttccatccct accacggcat tgaggtcgac
     3061 gacgtcgtcg acgtccgaat cgaaaatcag ctcgagcccc aggagatccc ggtagaacgc
     3121 gagcgagcgt tccatgtccg ctacgctgat gccggtgtga ttaacccgat tgactgtccc
     3181 cataactcaa ccccaaaacg gactcttcgc cgtcacctgc ctgcgcgccg gcaaaaacgg
     3241 tgaccgcgtg aatatacgcc cgcaccggcg ggaatgacag acgatcgatt tgaccctccg
     3301 ctcacgctat gctgagcgtc cctggacaca gcgtttccct agaaaaacga acgacac
//
LOCUS       DBNHQR010000484         3789 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1566484, whole genome shotgun sequence.
ACCESSION   DBNHQR010000484 DBNHQR010000000
VERSION     DBNHQR010000484.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1566484"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="AC16VR_10945"
     CDS             complement(<1..342)
                     /locus_tag="AC16VR_10945"
                     /inference="COORDINATES: protein motif:HMM:NF027439.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003860 - 3-hydroxyisobutyryl-CoA
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6011717.1"
                     /translation="MELKATEYSVASGIATVRLNRPHRLNAWTGRMHSEYRWCIDRAE
                     RDPAVRAIVVTGNGRGFCVGGDAEALEGHARKGGYDPGTPARLAAPGYGRAPEFDASF
                     AYHYGLSKPVIA"
     gene            complement(376..1461)
                     /locus_tag="AC16VR_10950"
     CDS             complement(376..1461)
                     /locus_tag="AC16VR_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR6011718.1"
                     /translation="MMVMRILALVLMFGLAGCGAPQQTAPEAVPDPDKVYRWKLITTW
                     PKNLPALGMAPERLAENLRVMSRGRLDIRVYGAGELVGAFEVFDTVRQGTAEMGHGAA
                     YYWRGKLPVAAMFATVPFGMMAQEMNGWLRYGGGMELWREAYEPFGLVPLAAGNSGVQ
                     MAGWFNKEINSLEDLQGLKMRIPGLGGEVLKRAGGVPVSMAGSEIYTAMQTGVIDATE
                     WVGPYNDLAMGLQDVARYYYYPGWHEPGPTLEAIVNKAAWDSLPEDLQRMVEVAAAAA
                     NEDLLSEFTARNASALKTLLSKHNVELRRLPDDVLAKLKVESEAVVVEATGDSDLARR
                     IRESYGRFLSDVREYHAISEQAYINAR"
     gene            complement(1479..3656)
                     /gene="uvrD"
                     /locus_tag="AC16VR_10955"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(1479..3656)
                     /gene="uvrD"
                     /locus_tag="AC16VR_10955"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016971704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HIR6011719.1"
                     /translation="MDVTNILEHLNKDQREAVSAPLGNALVVAGAGSGKTRVLVHRIA
                     WLIEAERVSPHGILAVTFTNKAAAEMRARIETLLDLSVRTMWVGTFHGIAHRLLRMHW
                     QEAGLKQNFQILDADDQLRLVKRVMRALDIDEQKWPARQAVWFINAQKDEGRRAKDVA
                     AGDDLFQITHKKIYESYESLCESGGLVDFAELLLRSHELWLNRSDLLAHYQERFQNIL
                     VDEFQDTNTIQYAWLRVLAGSSANIMAVGDDDQSIYGWRGARIENIHQFSQDFAGASL
                     IRLEQNYRSTATILKAANALIRQNVDRLGKELWTEGGDGEPIRVYSAYNDLDEARFVA
                     ERTAEALASGASADDVAVLYRSNAQSRVLEEAFLRAQIPYRIYGGVRFFERAEIKNAL
                     AYMRLTVDRHSDTAFERVVNTPARGIGDKTVEAIRAQARARNVSLWQACKEALAENVL
                     ASRTAAKIADFLGLIDGLAEQTAELDLHEVADACIQSSGLLEYHMRERGERGLARKEN
                     LEELVSACRQFSGEQVFPLLADAAEQTSILEEFLDQVALDAGDRQAEAGPAVQMMTLH
                     SAKGLEFPMVFLAGMEEGLFPHRMSAEEPGRMEEERRLCYVGITRAKQTLYLTHAETR
                     RLHGTDTYNRPSRFLLEIPTELVHEVRMGGAVQRPYGRPAEAGHAVGAEGGLRMGQRV
                     LHAKFGEGVVLQAEGSGERARVQVNFAGAKWLMLGYANLQALD"
BASE COUNT          633 a         1298 c         1164 g          694 t
ORIGIN      
        1 tgcgatcacc ggtttggaaa gcccgtagtg ataggcaaag ctggcgtcga attcgggcgc
       61 ccgaccgtat cccggcgctg cgagccgagc aggcgttccc ggatcgtagc cgcccttgcg
      121 cgcgtgccct tccagcgctt cggcgtctcc cccgacgcag aaacccctcc cgttgcccgt
      181 gacgacaatc gcgcggaccg ctgggtcacg ctccgcgcga tcgatgcacc agcggtactc
      241 gctgtgcatt cgacccgtcc acgcgttgag gcgatgaggc cggttcaggc gcacggtggc
      301 gatgccggac gcgaccgagt actccgtcgc cttcagctcc aacgcgacct ccccgccggc
      361 cggtcgcgca gaggctcagc gggcattgat gtaggcctgc tcgctgatgg cgtggtactc
      421 gcgcacgtcg ctgagaaacc gcccgtagga ttcacgaata cgacgggcaa gatcggagtc
      481 accggtagcc tcgaccacca ccgcctcgga ctcgacttta agctttgcca gcacgtcgtc
      541 cggcaagcgc ctgagctcca cgttgtgctt cgaaagaagc gttttgaggg cgctggcatt
      601 gcgggcggtg aactcgctca ggagatcttc gttggcggca gccgcggcga cctccaccat
      661 tctctgcaga tcctcgggca acgaatccca cgccgctttg ttgacgatgg cttccagggt
      721 ggggccgggc tcgtgccagc ccgggtagta gtagtagcgt gccacgtcct gaagccccat
      781 ggccaggtcg ttgtaggggc ccacccattc cgtggcgtcg atgacgcccg tctgcatggc
      841 cgtgtaaatc tccgaccccg ccatggacac cggcacgccc ccggcacgct tgagcacctc
      901 gccgccgagc cccgggatgc gcatcttcaa tccctgaaga tcctccaggg agttgatttc
      961 cttgttgaac cagcccgcca tctgcacgcc gctgttaccg gcggcgaggg gcacgaggcc
     1021 gaacggttcg taggcctcgc gccacagctc catgccgccg ccgtagcgca gccagccgtt
     1081 catctcctgg gccatcatgc cgaaaggaac cgtggcgaac atggcggcta ccgggagctt
     1141 gccgcgccag tagtaggcgg ctccatggcc catctcggcc gtgccctggc gtaccgtatc
     1201 gaacacctcg aacgcgccga ccagctcccc ggcgccgtag acccggatgt ctagccgacc
     1261 ccgactcatg acccgcagat tttcggccag gcgctccggc gccatgccga gcgccggaag
     1321 gtttttcggc caggtcgtaa tgagcttcca gcgatagacc ttatcgggat cgggaaccgc
     1381 ttcgggcgcc gtctgctgcg gcgcgccgca gccggcgagg ccaaacatga gcaccagggc
     1441 caggatccgc atcaccatca tgtcgctctt ctccagtttc aatcgagggc ctgcaggttt
     1501 gcatagccca gcatcaacca tttcgcgccg gcgaagttga cctgcacccg ggcgcgctca
     1561 ccggaaccct cggcctggag caccacgccc tcgccgaact tggcgtgtag cacccgctga
     1621 cccattcgca ggccgccctc agccccgact gcgtgcccgg cttctgcagg ccggccgtag
     1681 ggtcgctgca ccgcgccgcc catgcgcacc tcgtgaacga gctccgtcgg aatctccagc
     1741 aggaagcgcg acggccggtt gtaagtatcc gttccgtgca ggcgccgagt ctccgcatgg
     1801 gtgaggtaga gcgtctgctt ggcgcgggtg atgcctacgt agcacagccg cctttcctct
     1861 tccatgcgcc cgggctcttc ggcggacatg cggtggggga agaggccttc ctccatcccc
     1921 gcgagaaata ccatggggaa ctccagcccc ttggccgagt gcagggtcat catctgcacc
     1981 gcaggacccg cttccgcctg gcgatcaccc gcgtccagag ctacctggtc gagaaactcc
     2041 tcgagtatcg acgtctgctc ggcggcgtcg gccaggagag ggaatacctg ctcgccgctg
     2101 aactggcggc aggcgctgac cagctcctcc aggttctcct tgcgagccag gccacgctcc
     2161 ccccgctcgc gcatgtggta ctccagaagg ccgctggact gaatgcaggc gtcggcgacc
     2221 tcgtgcagat ccagctcggc cgtctgctcc gccagcccgt ctatgagccc gaggaaatcg
     2281 gcgatcttgg ccgccgtccg gctggcaagc acattctcgg caagggcttc cttgcacgcc
     2341 tgccacaggg acacgttgcg ggcgcgcgcc tgggcccgga tcgcctccac ggtcttgtcc
     2401 ccgataccgc gagccggggt gttcacaacc cgctcgaagg ccgtgtccga atgccggtcc
     2461 acggtcagac gcatgtaggc aagcgcgttc ttgatctccg ccctttcgaa gaaccgcacg
     2521 ccaccgtaga tgcgataagg aatctgcgcc ctcaagaatg cttcttccag cacccgagat
     2581 tgggcgttcg agcggtacag aacggcgacg tcatcggcgc tggcccccga ggccagcgct
     2641 tcggcggtgc gttcggccac gaagcgcgct tcgtcgaggt cgttgtaggc ggaatagacg
     2701 cgaatgggtt ctccgtcccc gccttcggtc cagagttcct tgccgagccg gtcgacgttc
     2761 tgacgtatga gcgcgttcgc cgctttgagg atggtggccg tcgagcggta gttctgctcc
     2821 agccggatca gcgatgcccc agcaaagtcc tgggagaact gatggatgtt ctcgatccgc
     2881 gcgcctcgcc agccatagat tgactgatcg tcgtcgccca cggccatgat gttcgccgag
     2941 cttcctgcca gcacccgcag ccacgcgtac tgtatcgtgt tggtgtcctg aaactcgtcg
     3001 acgagaatgt tctgaaaccg ctcctgatag tgggcgagga gatccgagcg gttgagccac
     3061 agctcatggc tgcgcagaag cagctcggca aaatccacta ggccgccgct ttcgcaaagg
     3121 ctttcgtagc tctcgtagat cttcttgtgg gtgatctgaa acagatcgtc acccgcggcg
     3181 acgtcctttg cgcgccgccc ctcatccttc tgggcgttga tgaaccagac cgcctggcgg
     3241 gcgggccatt tctgctcgtc gatgtccagc gcccgcatga cgcgcttgac caggcgcagc
     3301 tgatcgtcgg cgtcgagaat ctgaaagttc tgtttgaggc ccgcttcctg ccagtgcatc
     3361 cgaagcagcc gatgggcgat gccgtgaaac gtaccgaccc acatggtgcg aacggagaga
     3421 tccaggagcg tttcgatcct agcccgcatc tccgccgccg ctttgttggt gaaggtgacg
     3481 gccaggatgc cgtgggggga tacccgctcg gcctcgatca gccaggcaat ccgatgaacc
     3541 agcacccgcg tcttgccgct gccggcgcca gccaccacca gggcattgcc cagaggcgcc
     3601 gagaccgcct cccgctggtc cttgttcaga tgctcgagaa tgttcgtgac gtccactgct
     3661 acccgcccta agcctgtcca agggacgggg gttcagggaa ccgcccggcg cggcttaggg
     3721 ccggaacgga ctactccacc gtgaccgact tagccagatt ccttggctgg tccacgtccg
     3781 tgcctttga
//
LOCUS       DBNHQR010000485         1896 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1123835, whole genome shotgun sequence.
ACCESSION   DBNHQR010000485 DBNHQR010000000
VERSION     DBNHQR010000485.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1123835"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="AC16VR_10960"
     CDS             complement(<1..208)
                     /locus_tag="AC16VR_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011720.1"
                     /translation="MTRPAAPRLPSGLLLALLLAPQVASGHGGVVLEEDQCVLKMDFL
                     LAHFTGYQPESRGSEEFCEDIPEVG"
     gene            306..1289
                     /locus_tag="AC16VR_10965"
     CDS             306..1289
                     /locus_tag="AC16VR_10965"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HIR6011721.1"
                     /translation="MATIAILGLGNFGTALARVWLLGGHDVRGWTVEEDVFDAIRSKG
                     SNSKYLDGIDLTGLQVSMELGESVPGAELVVLALPSHVVLHVADDVIPLLTPDQVVLD
                     LAKGIAGEELVSDAIQAKLAAAGKTNPLAVITGPTIAPELASGVLTTALVASEDESLA
                     SRLAEQLSTSTFSLKAASDPRGAELWGGFKNVIALGCGIADGLDEGSGDNLKAAIFTA
                     GFAEGNRLLTALGAAPETAFTPAGIGDLFVTASSPHGRNRSLGQKLGAGQTLEEAMAG
                     SVMVAEGVRATRMFAELAAARSVTTPFIAALTDLLDGNCTAPDCVRRLMAI"
     gene            1385..1684
                     /locus_tag="AC16VR_10970"
     CDS             1385..1684
                     /locus_tag="AC16VR_10970"
                     /inference="COORDINATES: protein motif:HMM:NF018717.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HIR6011722.1"
                     /translation="MAAFFVAQYTVNNPALYAEYQQGAGPTLATYGAELVSFDVGAET
                     VEGNPPGPQTVIIKFESTDAAKAWYNSAEYQAVVGKRLEATDGFAVIAQSMNAGG"
BASE COUNT          334 a          641 c          618 g          303 t
ORIGIN      
        1 cccccacctc gggaatgtcc tcgcagaatt cctcggatcc cctggattcg ggctggtagc
       61 cggtgaagtg ggcgagaagg aagtccatct tcagaacgca ctggtcctcc tcgagcacta
      121 ccccgccatg acccgatgcg acctggggcg ccagcagcag ggcgagcagc agcccgcttg
      181 ggagacgagg agcggccggt ctggtcattg ggagtttgat gcgttcttcc tccggcgtct
      241 tgcctgatcg gctaactttg gcatacaaca ggcaacgatc caacggtgac caaggggcgg
      301 cagacatggc cacgatcgcg attctcgggc tcggcaactt cggcacggcc ctggcccggg
      361 tctggctgct aggcgggcac gacgtccgcg gatggacggt ggaggaagac gtgttcgacg
      421 ccatccgctc caaaggcagc aatagcaaat acctcgacgg catcgacctc accggcctgc
      481 aggtatccat ggagcttggc gagtcggtcc ccggggcgga gctcgtggtg ctcgccctgc
      541 cctcccacgt ggttcttcac gtcgccgacg acgtcatacc tctgctgacg ccggatcagg
      601 tggtgctgga tctcgccaag ggcatcgccg gcgaggagct cgtttccgat gccatccagg
      661 ccaagctggc ggcagccggc aagaccaacc cgctcgcggt gatcaccggc ccaaccatcg
      721 cgcccgagct tgccagcggc gtgctcacca cggcgctcgt ggccagcgaa gacgaatcgc
      781 tggcgagccg cctcgccgag cagctatcca cctcgacttt ttcgctgaag gcggccagcg
      841 acccgcgcgg cgcggagttg tgggggggct tcaagaacgt gatcgcgctg ggctgcggaa
      901 tcgccgacgg gcttgacgag ggaagcggcg acaacctgaa ggcggcgatc ttcacggccg
      961 gttttgccga aggtaatcgc ctgctcaccg cgctgggggc cgcgccggag acggcgttta
     1021 ctccggccgg catcggcgat cttttcgtca ccgcatcgtc accccacggc cgcaaccggt
     1081 ccctgggcca gaagctgggc gccgggcaga ccctggagga agccatggcc ggcagcgtca
     1141 tggtcgcgga gggcgttcgc gccacgcgaa tgtttgccga gctcgccgca gcccggagcg
     1201 tgaccacgcc gttcatcgca gcgcttacgg acctcctcga cggaaactgc accgcgcccg
     1261 actgcgtccg ccgactgatg gcgatctgaa gcagacggcc cctctcggca acggccaatt
     1321 gtggctgcgg ggtaatcggg taccatagcc ggtctcatca catcgcaaag gggaggagcc
     1381 aaacatggcc gcattcttcg tcgctcagta caccgtcaac aacccggccc tttacgccga
     1441 gtaccagcag ggcgccgggc ctactctggc cacctacggg gctgagctgg tctctttcga
     1501 cgtaggcgcc gagaccgtcg agggcaaccc gcccggcccg cagacggtga tcatcaagtt
     1561 cgaatccacg gacgccgcca aagcctggta caactcggct gagtatcagg ccgtggtcgg
     1621 caagcgcctg gaagccaccg acggctttgc cgtcatcgct cagtcgatga acgcgggcgg
     1681 ctgatcgcgc accgcacggc gcggggttga cggccgctca gccgtccagc tcaccgcgcc
     1741 gtgccagatc cctctccagg tcccgacccg ccatccagta gtacacggtg gccacgacgt
     1801 tgagcagcag cacgcagatc agcgcgtagc gcagcgagtc ggtgccgagg tccccgaagg
     1861 cgttgaaccc atcgctgagg atgcccgtga cctggg
//
LOCUS       DBNHQR010000486         2499 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_300908, whole genome shotgun sequence.
ACCESSION   DBNHQR010000486 DBNHQR010000000
VERSION     DBNHQR010000486.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_300908"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1928)
                     /locus_tag="AC16VR_10975"
     CDS             complement(<1..1928)
                     /locus_tag="AC16VR_10975"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HIR6011723.1"
                     /translation="MTSGKPADSNFWPHCASLVGQDAATELRVADVLSRMTLEQKVGQ
                     MIQAEIAAVTPAQAAEHHLGSVLNGGGSFPNGDKHASVADWVALAESYRAASSDAGPG
                     RLGIPLIWGTDAVHGHNNVYRATIFPHNIGLGAAGDPDLIEEIGAATAREIAATGIGW
                     TFAPTLAVVRDDRWGRTYESYSEDPQIIRAYAGRMVTGLQGPAGTDAFLGAGRVVATA
                     KHFLGEGGTEGGVDQGENNADETTLRDVHGQGYVTALGSGAQTVMATFNSWRGVKVHG
                     HRYLLTDVLKGQMGFEGFVVSDWNGFKQVADCRQEACQLSINAGVDMLMIPDDWQEVI
                     GDLVALVEDGQIPMSRIDDAVTRILRVKARAGLLDPAESSDRPHTDPAVVGCAAHRDV
                     ARRAVRQSLVMLKNRGGMLPLDPSLHVLVAGDGADDIGKQSGGWTLTWQGTENENGDF
                     PGASSIYDGISAAVTNAGGRATLSPDGSWSERPDVAVVVFGEDPYAEGQGDRAHLSFS
                     ALFPEGLALLKKLKGDGIPVVSVFLTGRPLWVNPELNASDAFVVAWLPGSEGAGVSDV
                     LFRTVGGEVAHDFTGRLAFSWPRSSLQATVNRHDADYDPLFPYGFGLGAADVDTLGDE
                     LIEEDEALGLAADESIGIL"
     gene            complement(2128..>2499)
                     /locus_tag="AC16VR_10980"
     CDS             complement(2128..>2499)
                     /locus_tag="AC16VR_10980"
                     /inference="COORDINATES: protein motif:HMM:NF024770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR6011724.1"
                     /translation="TLVMQGYFDFESGQRCARKLLELPNPPTAIFASNDDMAAGVIFT
                     ARERGIGVPDELSVAGFDDSPAASHTWPPLTTVKQPIVEMAARATQRLIGQLRGVGEE
                     GDEEIFTCELIVRRSTAKRGD"
BASE COUNT          420 a          849 c          818 g          412 t
ORIGIN      
        1 aggatgccga tgctctcgtc ggccgcaagg ccgagcgcct cgtcttcctc gatgagctcg
       61 tcaccgaggg tatcgacgtc tgcggcaccg agcccgaagc cgtaggggaa cagcgggtcg
      121 taatccgcat cgtggcgatt caccgtggcc tgcagcgagg agcgcggcca ggaaaaggcc
      181 agccgtccgg taaagtcgtg agcgacctcg ccacccacgg ttcgaaagag cacgtcgctc
      241 acgccggcgc cctccgagcc cggaagccag gccaccacga aggcgtcgga agcgttcagc
      301 tcggggttga cccagagagg ccggcccgtg agaaagacgc tcaccaccgg gatgccgtcg
      361 cccttcagtt tcttgagcag cgcaagccct tcgggaaaca gggcggagaa gctcagatgg
      421 gcgcggtctc cctgcccctc ggcgtacggg tcctctccga acaccaccac cgccacatcg
      481 ggccgctcgg accagctgcc gtcaggtgac agcgtggccc ggccgccggc gttggtaacc
      541 gcggcggaga tgccgtcgta tatcgagctc gccccgggga aatcgccgtt ctcgttctcg
      601 gtgccctgcc aggtcagcgt ccagccgccc gactgcttgc cgatgtcgtc cgccccgtcg
      661 cccgctacga gaacgtgcag ggaagggtcg agaggtagca tgccgccgcg attcttgagc
      721 atcaccaggg actggcgaac ggcccgtcga gcgacgtcgc ggtgtgcggc gcagccaacc
      781 acggccggat cggtatgtgg ccggtcggac gactctgcgg ggtcgagcag gccagcgcgg
      841 gctttgacgc gcaagatccg ggtgaccgca tcgtcgatgc gggacatggg aatttggccg
      901 tcctccacca gagccaccag atcgccgatg acctcctgcc agtcgtcggg gatcatcagc
      961 atgtcgacgc cggcgttgat cgacagctgg caggcttcct ggcggcaatc ggcgacctgc
     1021 ttgaagccgt tccagtcact gaccacgaac ccctcgaagc ccatctggcc tttgagcacg
     1081 tcggtgagca ggtaacggtg accgtgtacc tttacgcccc gccagctgtt gaacgtggcc
     1141 atgaccgtct gggcgccgga gcccagggcc gtgacgtaac cctgaccgtg aacgtcgcgc
     1201 agggtcgtct cgtcggcgtt gttctcgccc tggtccacgc ctccttcggt gccgccctcg
     1261 ccgagaaagt gcttcgccgt ggcgaccacg cgcccggccc cgaggaacgc gtcggtaccg
     1321 gccggaccct gcaggccggt gaccatccgc ccggcatagg cgcggatgat ctggggatct
     1381 tcggagtagc tttcgtaggt gcggccccag cggtcgtcgc gaaccaccgc cagggtcggt
     1441 gcgaacgtcc atccgatgcc ggtggcggca atctctctgg ccgtggccgc gccgatctct
     1501 tcgatcaggt cggggtcccc ggcggctccc aggccgatgt tgtgaggaaa tatggtcgcg
     1561 cgatagacgt tgttgtgacc gtgaaccgca tcggtgcccc agatcaacgg gatcccgagc
     1621 cggccaggac ccgcatcgct ggaggccgcg cgataactct cggccagcgc cacccagtcc
     1681 gccaccgatg cgtgcttgtc accgttcgga aacgaaccgc ctccgttgag aacggaccca
     1741 agatgatgct cggccgcctg ggccggggtg acggcggcaa tctccgcctg gatcatctgg
     1801 ccgaccttct gttccagggt catccgggac agcacgtcgg cgacccgaag ctccgttgcg
     1861 gcatcctgcc ccaccaggct ggcgcagtgc ggccagaaat tcgaatcggc gggtttaccg
     1921 ctggtcatcg tgatcctcaa aaacatcagg ccagagatac gcgacgcgcc gccacgaccc
     1981 cactggccgc gaatatgaca acgctgtcat tttgacccga agttaacccg attcccggcg
     2041 agcttgtcaa acgtttgcag ggcagatggc cggcacacgg cccgcggcgg atctcggcca
     2101 ggcgtctcgg cccgccgtca gcccgcttca gtcgccgcgc ttggcggtgg agcgtcgcac
     2161 gatgagctcg caggtgaaga tttcctcgtc gccctcttca cccacacccc gcagctggcc
     2221 aatgagccgc tgggtcgctc gtgcagccat ctccacgatg ggctgcttga cggtcgtcag
     2281 cggcggccag gtgtgcgacg ccgccggaga gtcgtcgaat ccggccacgg agagctcgtc
     2341 gggaacgccg atgccgcgct cacgcgcggt gaaaatgacg cccgccgcca tgtcgtcgtt
     2401 gctggcaaag atggccgttg gcgggttcgg cagctctagg agcttccggg cgcagcgctg
     2461 accggactcg aaatcgaagt agccctgcat taccagggt
//
LOCUS       DBNHQR010000487         1699 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_301003, whole genome shotgun sequence.
ACCESSION   DBNHQR010000487 DBNHQR010000000
VERSION     DBNHQR010000487.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_301003"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="AC16VR_10985"
     CDS             <1..312
                     /locus_tag="AC16VR_10985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/IscA family protein"
                     /protein_id="HIR6011725.1"
                     /translation="AAAAHIRSQIATSGHKYLRLGVKESGCNGYMYTLDYLDEPAEND
                     RAFEVADSLSLYVSTDDLPMVSGTEVDYVTEGLNSSLKFKNPNAESHCGCGESFSIAG
                     S"
     gene            327..851
                     /gene="sufT"
                     /locus_tag="AC16VR_10990"
     CDS             327..851
                     /gene="sufT"
                     /locus_tag="AC16VR_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019340522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HIR6011726.1"
                     /translation="MEKRIIATTRECPARLVPTGDPITIPSNTFVTLTQNLGGTYTVI
                     MNGNMARVDSADADALGFEPQTLEFEPPADGKVDMEQVKAALATVYDPEIPVNVVDLG
                     LIYDCRLTDRHVHIAMTLTAPGCGMGPVLVEDIKSRVGRVPYVESVDVELVFDPPWSR
                     DMMTAEAQLELGLF"
     gene            855..1271
                     /locus_tag="AC16VR_10995"
     CDS             855..1271
                     /locus_tag="AC16VR_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008169334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HIR6011727.1"
                     /translation="MDIGDIRDSFAFFDSWEDKYRFVIEIGRDLPPLDAALKTEDNLV
                     RGCQSQVWLVAAYDPVADRLTFAIDSDAHIVRGLIAIILAAFDKRSPAEILAFDVEAL
                     FEELELLQHLSPSRGNGLRAMVGRIRGIAAENAAGA"
     gene            complement(1303..>1699)
                     /locus_tag="AC16VR_11000"
     CDS             complement(1303..>1699)
                     /locus_tag="AC16VR_11000"
                     /inference="COORDINATES: protein motif:HMM:NF021365.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HIR6011728.1"
                     /translation="DWGLPLFEQRGGDLGERMAAAMAHGCARNVATLIVGIDCPCIDG
                     AYVRAAAQTLLTTDLVLAPAEDGGYGLIGLRRPMPELFRGVAWGSSSVLAETLVKAER
                     LRLRYELLEAVWDVDDAADWERFLESGYG"
BASE COUNT          328 a          567 c          510 g          294 t
ORIGIN      
        1 gccgcggctg cccacatccg gagccagatc gccacgtccg gccacaaata tctgcggctg
       61 ggcgtcaagg aaagcggctg caacggctac atgtacactc tggattacct ggacgagcct
      121 gcagagaacg accgggcgtt cgaggttgcc gattctctga gcctctacgt gagcaccgac
      181 gacctgccca tggtgagcgg cacggaggtc gattacgtga ccgagggcct gaactcgagc
      241 ctcaagttca agaaccccaa cgccgagtct cactgcggtt gcggggaaag cttctccatc
      301 gcaggttcct agagagcgac cggccggtgg aaaagcgcat catcgccacc accagggagt
      361 gcccggcaag gctcgtcccg acaggtgatc ccatcacgat cccatccaac acctttgtga
      421 cgttgaccca gaatctcggc gggacgtaca ccgtgatcat gaacggcaac atggctcgtg
      481 tggacagtgc cgatgctgac gctttggggt tcgagcccca gaccctcgaa ttcgagcctc
      541 ctgcggatgg caaggtcgac atggagcagg tcaaggccgc cttggcaacc gtttacgatc
      601 cggagatccc cgtcaacgtc gtcgatctcg ggctgattta cgattgcagg ctgaccgacc
      661 ggcacgttca catcgccatg accctcaccg caccgggttg cgggatggga cccgtgctgg
      721 tcgaggacat caaatccagg gtcggaaggg ttccctacgt cgaatccgtg gacgtggagc
      781 tcgtcttcga tccgccctgg tcccgggaca tgatgaccgc ggaagcccag ctggagctcg
      841 gcctgttctg atccatggac atcggcgaca tccgcgacag ctttgcgttt ttcgattcct
      901 gggaggacaa ataccgcttc gtcatcgaga tcgggcgtga ccttccaccg ctggacgccg
      961 ccctgaagac cgaggacaac ctggtgcgcg gctgccagag ccaggtgtgg ctggtagccg
     1021 cctacgaccc ggttgccgac aggctcacct tcgccatcga cagtgacgcg cacatcgtcc
     1081 ggggactgat cgcgatcatc cttgctgctt tcgacaaacg ctcgcccgcc gagattctgg
     1141 cattcgacgt ggaagccctc ttcgaggagc tggagctgct ccagcatctc agccccagcc
     1201 ggggaaacgg gttgcgtgcg atggtcggca ggatcagagg catcgctgcc gagaacgcgg
     1261 caggcgcctg atcgaagcac gcatcgcccg gctccccaag attcacccgt agccggactc
     1321 gagaaatcgc tcccagtccg ccgcgtcatc cacgtcccag acggcttcga gcagctcgta
     1381 tcgaagccgc agcctctcgg ctttcaccag cgtttcggcg aggacgctgc tgctgcccca
     1441 cgccacgccc cggaacagct cgggcattgg ccgccgcagc ccgatcagcc cgtaaccgcc
     1501 gtcctccgcg ggtgccagaa cgagatccgt cgtgagaagc gtttgggccg cagcgcgcac
     1561 ataggcgccg tcaatgcatg gacagtcgat tccaacgatc agcgtcgcaa cgttccgggc
     1621 acagccgtgg gccatggctg cggccatgcg ctctcccaga tcgccaccac gctgctcgaa
     1681 caacggcagg ccccagtcc
//
LOCUS       DBNHQR010000488         7445 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_933660, whole genome shotgun sequence.
ACCESSION   DBNHQR010000488 DBNHQR010000000
VERSION     DBNHQR010000488.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7445)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7445)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7445
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_933660"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="AC16VR_11005"
     CDS             <1..537
                     /locus_tag="AC16VR_11005"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR6011729.1"
                     /translation="LVAAGEWWRCITALTLHGDLGHIVANSLFGSLFGLYVGRYLGSG
                     FGWLLILASGALGNAMNAWIQPDAFSSIGASTASFGALGVSAAFVWRRGYYRNRDWRR
                     SLAPIFAGIALLAFTGTGGENTDVVAHFTGFAAGALLGAAAAAFDIRRLGVSGQYLAG
                     AAAITLVVTAWSFAGTVG"
     gene            complement(538..1116)
                     /locus_tag="AC16VR_11010"
     CDS             complement(538..1116)
                     /locus_tag="AC16VR_11010"
                     /inference="COORDINATES: protein motif:HMM:NF016270.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HIR6011730.1"
                     /translation="MSHITTTFLRGLGVVLPLSLTIWFVVWLAVTTESLLRSVFLVIL
                     PEGLYLPGLGLIFGIVMVYGVGVLAQIFFLQSLWRHARNLLDRIPLVKTVYTAISDFY
                     QFFSSGPADGAARVVSVDVSDDVSVIGFVTGPSPSFMDQGGSRRIAVYFPMSYQIGGY
                     TLMVPEDRVSPLDIGVEDAMRVILTASVRSRR"
     gene            1253..2293
                     /locus_tag="AC16VR_11015"
     CDS             1253..2293
                     /locus_tag="AC16VR_11015"
                     /inference="COORDINATES: protein motif:HMM:TIGR03559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6011731.1"
                     /translation="MKLGLALGYSGAYLNIPLKKVQLAERLGYDSVWTAEAYGSDAIT
                     PLAYIAAHTERIRLGTGVIQLAARTPANAAMAFATLDQLAGGNRAICGIGVSGPQIVE
                     GWYGQPWGRPYYRIKDYVAIMKKIFAREEPVTHAGKEISLPYQGEGALGIGKPLKSIL
                     HMNPNIPIFLGTGMESTVRLTAEVADGWLPLGFVPETAALYDGWIDEGLAKGGKTSEQ
                     FERQAGTSVQVTDDVKSALDRLKPGIALYVGGMGHKTKNFHKEMMIRRGFGEAAERIQ
                     ELYLAKRKDEAIAAVPDEFVDQGALVGPKERIARRFRDWEDSGLTGLTISGDEEAVRF
                     MAECARLNFAAE"
     gene            complement(2305..3639)
                     /locus_tag="AC16VR_11020"
     CDS             complement(2305..3639)
                     /locus_tag="AC16VR_11020"
                     /inference="COORDINATES: protein motif:HMM:NF024895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR6011732.1"
                     /translation="MSRHWCQGALASMLGCALLWSSCAAADQAGGWYAGVGIGWNHAE
                     NMELDELGGVVDYDFGAPVWSAALGWAFGPDWRVEVEASRQRNDVELVSFSAPVSASA
                     AALDPAPENAFSATTLAINAVREFAVGRDFRPYLGAGVGLAWTELDITDGSIEAQIDP
                     EPRDPLVDDQATSFAFQLIGGISLPLTRRMDLGVEYRYWQAPSVKLKEFTGTKLDTKH
                     AVHSGWLQLRYRSGGVERSRPAASPRPSPRGFYLTTFAGAGFSVDVEIDPGQTNFDAL
                     DVGPVAAFGVGYALSRRWRFELETAFRKNEVELLDFGLRFGSKQGSGRIRSTSLLASG
                     IYQFFPEARTRPYLGVGAGLVWADYDVDVLDELFVDDKDQSYAAEFRLGAEVSVSQRL
                     TVSAEYRYWISSDLKMTGPDGGPVEAYQQTHSMMVGLRYALGSGRSAGASGR"
     gene            complement(3680..4519)
                     /locus_tag="AC16VR_11025"
     CDS             complement(3680..4519)
                     /locus_tag="AC16VR_11025"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6011733.1"
                     /translation="MAYDYELLDVDIDGRVATVTVSNPPINLITVALYRELASLGAEL
                     RADPNLTVVVMKSADPDFFLAHFDVETILSFPVDGEAQRSAELSEYHLMCERFRTMDK
                     VTIAQIEGRVGGGGSELAASFDMRFGVRGRTKINQMEVPLGILPGGTGTQRLPRLVGR
                     GRALEIVLGGADLDAETAESWGYLNRIFEPEEIGGFVAALARRIASYPVEAVRLAKEA
                     VLGAETPVHQGLSNEAYLFQRLLRTEGAQRNMRRFLQIGGQTREGELRMGELCAELGE
                     ESR"
     gene            4621..5802
                     /locus_tag="AC16VR_11030"
     CDS             4621..5802
                     /locus_tag="AC16VR_11030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIR6011734.1"
                     /translation="MDGRQQAIVNRIDELADELLAVSHAIHANPELAFEEHDACALLA
                     STARRFDLPVETGVFTLETAFQGSFGGRTSGPTVAILAEYDALPGIGHACGHNLIATS
                     ALGAALGLKAVDDELPGTVRLIGTPAEERGGGKELMARAGAFDGVDAAMMIHPAGVNL
                     ATMPCICVAEVEVIYHGRSAHASAMPHKGINALDGLLLAYQAISNLRQHIRDHERIHG
                     IVTEGGLAPNIVPDRAAGAFYVRAANEQDLARLKPRVQACFEAGAKGSGCELEVNWAN
                     VDYLDLNTNWPLANRFQAHGERLGREFLKPASLGGAGSTDMGNVSYRVPSIHPMLACA
                     PPNVVIHNPEFAKWARSEQGDAAAIDGAKALALTAADYLLDGSLQAEVNKAFAISKGL
                     A"
     gene            5819..6358
                     /locus_tag="AC16VR_11035"
     CDS             5819..6358
                     /locus_tag="AC16VR_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003839 - gamma-glutamylcyclotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061928 - glutathione specific
                     gamma-glutamylcyclotransferase activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HIR6011735.1"
                     /translation="MNVWLFGYGSLIWRPEIAYVERRPARLHGWARRFWQGSHDHRGM
                     PRQPGRVVTLIEEEGGHCDGMAYLVDRGDVEAIFAALDHREKNGYRRFDVELHFSRYN
                     PAPATTGVVYIAPPGNHAFLGPAPLAEMARQISECAGPSGRNVDYLLELAEALRQLDA
                     QDAHVFELEAAVRHVSGSR"
     gene            6358..>7445
                     /locus_tag="AC16VR_11040"
     CDS             6358..>7445
                     /locus_tag="AC16VR_11040"
                     /inference="COORDINATES: protein motif:HMM:NF014790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6011736.1"
                     /translation="MDISFSAEHERFRDEVRVFLDGALDDELREAQRFCPGIFLDYEH
                     NIRWHRILHAKGWVAPAWPREYGGTGWDLTRRYIWSTETTLAGAPSLAPMGLGMCGPM
                     LIGYGTEAQKAYYLPRILSGEDYWCQGYSEPGSGSDLASLKLRADGDGDHYVLNGTKI
                     WTTHAHYANRMFCLVRTDDGGRPQQGITFLLLDMDTPGISVEPIVFASGTHEVNQVFF
                     DDVRVPKANRVGEENDGWTVAKYLLEFERGGGGSAAYRTALTRVRKLAGQVPADGASL
                     AEDPDFKRALDETDVKLQAIQYTEFRIMAALSRGQNPGPESTIMKNLGADIGQRLTEL
                     TVEAMGQYIAPHQPEARQVGSNVPVVGPP"
BASE COUNT         1353 a         2548 c         2354 g         1190 t
ORIGIN      
        1 ctcgtcgccg ccggcgagtg gtggcgctgc atcactgcgc tcaccctgca cggcgacctg
       61 ggccacatcg tagccaactc gctgttcggc tcgctgttcg gcctgtacgt cggccggtac
      121 ctcggctccg gcttcggctg gcttttgatc ctcgccagcg gcgcgctcgg caacgccatg
      181 aacgcgtgga tccagccgga cgcgttcagc tccatcggcg catccaccgc gagcttcggc
      241 gccctgggcg tgagcgcggc tttcgtgtgg cgccgcggct attaccgcaa ccgcgactgg
      301 cgccgcagcc ttgcgccgat attcgcgggt atagccctgc tggcgttcac cggcaccggg
      361 ggggagaaca ccgacgtggt cgcgcacttc accggctttg ccgcgggcgc cctcctcggc
      421 gccgcagccg ccgcgttcga catccgtcgt ctgggcgtct ccgggcagta tcttgcgggg
      481 gccgcggcca tcaccctcgt cgtgacggcc tggtcgttcg ccggcaccgt cggttgatca
      541 ccgcctgctg cgcacggagg cagtcagaat cacccgcatg gcgtcctcta cgccgatgtc
      601 caacggcgac acccgatctt ccgggaccat gagcgtgtag ccgccaatct ggtaactcat
      661 gggaaagtag acggcgatcc gccggctgcc gccttggtcc atgaaggatg gagaggggcc
      721 ggtcacgaag ccaatgaccg agacgtcatc tgagacgtcc acgctcacga ccctcgccgc
      781 gccgtcggcc ggcccgctgg agaagaactg gtaaaagtcg ctgattgccg tgtacaccgt
      841 tttgaccagc ggaattcggt ccaggagatt ccgggcgtgc cgccataggc tctgcaggaa
      901 aaagatctga gcgagcacgc cgaccccgta gaccatcacg atgccgaata tgaggcccag
      961 gcccggcagg tagaggccct ccggcaggat gaccaggaac accgagcgca gcagggactc
     1021 cgtggtcacg gccagccaga cgacgaacca gatggtcagg gaaaggggca gcacgacgcc
     1081 gagaccgcgc aaaaaggtag tggtgatgtg gctcatgacg atacgacctt gaagttgatg
     1141 cagcgaagtc tagccgttcg cgcgacgccg cgttcaaccg cgccgatggt cggcccgacg
     1201 ggcggccgcc acttgagtta agctccaggc aaaagttgcg caggagctga cgatgaaact
     1261 gggactggct ttagggtatt cgggcgcgta cctgaacatc ccattgaaga aggttcagct
     1321 ggccgagcgg ctgggctacg actccgtctg gacggccgag gcctacggtt ccgacgcgat
     1381 aacgccgctg gcttacatcg cggctcacac cgagcgcatc cggctcggca ccggcgtcat
     1441 ccagctcgct gcccgtacgc cggccaacgc cgccatggcg ttcgccacgc tcgatcagct
     1501 ggcgggcggc aaccgtgcca tctgcggtat cggggtttcc ggcccgcaga tcgtcgaggg
     1561 ctggtacgga cagccctggg gacggcccta ctaccggatc aaggattacg tggccatcat
     1621 gaagaagatc ttcgcccggg aagagcccgt cacccacgcc ggcaaggaga tcagcctgcc
     1681 ttaccagggc gagggcgccc tggggatcgg caaaccgctg aagtcgatcc tccacatgaa
     1741 cccgaacata cccatcttcc tgggaactgg catggagtcc accgtgcgcc tgacggccga
     1801 ggtcgccgac gggtggctgc cgctgggatt cgtgcccgaa accgcagcgc tctacgatgg
     1861 ctggatcgat gagggccttg ccaaaggcgg caagacgtcg gagcagttcg agcggcaggc
     1921 aggtacgtcg gtccaggtga ccgacgacgt gaaaagcgct ctggaccggc tcaagccagg
     1981 catcgcgctg tacgtcggcg gcatgggcca caagaccaag aacttccaca aggagatgat
     2041 gatcaggcgc ggcttcggcg aagccgcaga gcggatccag gagctctacc tggcaaagcg
     2101 caaggacgag gccatcgccg ccgttcccga cgagttcgtc gaccagggcg ccctggtggg
     2161 tcccaaggag cgcatcgcca ggcgtttccg ggactgggag gattcgggcc tcaccggtct
     2221 gaccatttcc ggggacgagg aagccgtgcg cttcatggcc gagtgcgcgc ggctgaattt
     2281 cgccgccgag tgagcccgcg gcgcctagcg gccggaagcg cccgctgagc gccccgaacc
     2341 cagggcgtac cgcagaccca ccatcatcga gtgggtctgc tggtaggcct ccaccgggcc
     2401 gccgtccggc cccgtcatct tcagatcgct cgaaatccag taccggtatt ccgccgacac
     2461 cgtcagccgc tgggacacgc tgacctctgc acccaggcga aactcggccg cgtaagattg
     2521 gtccttgtcg tccacgaaca gctcatcgag aacgtctacg tcgtagtcgg cccacacgag
     2581 ccccgcgccg acacccaggt aagggcgggt ccgggcttcc ggaaagaact ggtagatgcc
     2641 ggacgcgagc aggctggtgg agcggatccg gcccgagccc tgcttggaac cgaacctgag
     2701 cccgaaatcc agaagctcga cctcgttctt ccgaaacgcg gtttccagct cgaagcgcca
     2761 gcgccggctg agcgcgtaac cgaccccgaa cgccgccacc gggcccacgt ccagagcgtc
     2821 gaaattcgtc tgaccgggat cgatctcgac gtccacggag aatcccgcgc ccgcaaaggt
     2881 ggtgaggtag aagccccggg gcgaaggccg aggggacgct gccggccgcg accgctcgac
     2941 gccgccggaa cggtagcgca gctgcagcca gcctgaatga acggcatgtt tcgtatcgag
     3001 cttggtccct gtgaactcct tcagctttac ggacggcgcc tgccagtaac ggtactcgac
     3061 ccccagatcc atccttcggg tcaggggcag gctgatcccc ccgatcagct gaaatgcaaa
     3121 gctcgtggcc tggtcgtcga ctaaaggatc tctgggctcg ggatcaatct gcgcctctat
     3181 gctgccatcg gttatgtcca gttccgtcca ggccaacccg acgccagcgc cgaggtaagg
     3241 gcgaaagtcc cttcccaccg caaactcgcg caccgcgttg atcgccaacg tcgtcgcgga
     3301 aaaagcgttc tcgggagcgg ggtcgagggc ggcggcagac gccgataccg gcgcggaaaa
     3361 cgacacgagc tccacgtcgt tgcgctgccg cgacgcctcg acttccaccc gccagtccgg
     3421 cccgaaagcc caacccagcg ccgcagacca gaccggagcg ccgaagtcgt agtcgacgac
     3481 gccgcccaac tcgtccagct ccatgttctc ggcgtgattc cagccgatcc ccacgcccgc
     3541 ataccagccg ccagcctggt ccgcggcagc acaggagctc caaaggaggg cacaccccag
     3601 catggacgcc agagcgccct ggcaccagtg gcgactcacg ccgggatcga ccggcgaccg
     3661 gatggttgcc gatacggggt catcgggatt cctcgccaag ctctgcacac agctcgccca
     3721 tccgcagctc gccttcccgg gtttgcccgc cgatctggag gaacctgcgc atgttgcgct
     3781 gggcgccttc cgtgcgcagc agccgctgaa acaggtaggc ttcgttcgag agcccttggt
     3841 gaacgggggt ctcggccccc agaacggcct ctttcgccag cctcacggcc tccaccgggt
     3901 acgacgcgat ccgtcgcgca agcgccgcca cgaacccgcc gatttcctcc ggctcgaata
     3961 tgcggttcag atagccccag ctttccgccg tctcggcatc gagatcagcg ccgcccagca
     4021 cgatctccag cgcgcgcccc cgacccacca gcctaggcag acgctgtgtt ccggtcccgc
     4081 cagggagaat gcccaggggc acctccatct ggttgatctt cgttcgaccg cgcaccccga
     4141 aacgcatgtc gaagcttgct gccagctcgc ttccgccacc gccgacgcgc ccttcgatct
     4201 gcgcgatggt caccttgtcc atggtgcgga accgctcgca catgaggtgg tactccgaaa
     4261 gctcggcgga gcgttgggcc tcaccgtcca cggggaacga aagaatggtt tccacgtcga
     4321 aatgcgcgag aaagaagtcc gggtccgcac tcttcatcac caccaccgtt aggttcgggt
     4381 cggcccgaag ctcggcgccg agactcgcaa gctcgcggta gagggccacg gtgatgaggt
     4441 tgatgggcgg attgctgacg gttaccgtcg ctacccgccc atcgatgtcc acgtcgagca
     4501 gctcgtaatc gtaagccacg gatatctccc ttcctggtga tcaaagcgcc gcggcattcc
     4561 tctggaggcg tgccgcacga tgccccatac tcgagggcat tatatcgacc tgaccggaat
     4621 atggacggaa gacagcaggc catcgtcaac cgaatcgacg agctcgccga cgagctgctc
     4681 gccgtgtccc atgccattca cgccaatccg gagctcgctt tcgaggagca cgatgcctgc
     4741 gcgctgctgg caagcacggc caggcggttc gatcttccgg tggaaacggg cgttttcacg
     4801 ctggaaaccg ccttccaggg aagctttggc gggcgcacgt cggggcccac cgtggcgatt
     4861 ctcgcggagt acgatgctct gcccgggatc ggccacgcgt gcggccacaa cctcatcgcg
     4921 acatccgctc tcggtgccgc tctggggctc aaggcggtgg atgatgagct gcccgggacc
     4981 gtccgactca tcgggacccc tgccgaggaa cgcgggggcg gaaaggaact gatggcgcgg
     5041 gcgggcgcat tcgacggcgt ggacgcggcg atgatgatcc atcccgcggg ggtgaatctt
     5101 gcgaccatgc cctgcatctg cgttgccgag gtggaggtga tctatcacgg ccgctctgcc
     5161 cacgcctcgg cgatgcctca caagggcatc aatgccctcg acggcctcct gctcgcctac
     5221 caggccatat ccaacctgcg ccagcacata cgcgatcacg agcgcatcca cggcatcgtc
     5281 accgaaggag gcctggcacc gaacatcgtc cccgaccggg ccgccggggc cttctacgtg
     5341 cgagccgcca acgaacagga tcttgcccgc ctgaagccgc gcgtgcaggc atgtttcgag
     5401 gcaggcgcaa aagggagcgg ctgcgaatta gaggtcaact gggcgaacgt cgactacctg
     5461 gacctgaaca ccaactggcc tcttgccaac cgcttccagg cccacggcga gcgtctgggg
     5521 cgcgagtttc tcaaacccgc ttccctcggc ggcgcgggca gcaccgacat ggggaacgtc
     5581 agctaccggg taccatccat acatccgatg ctggcctgtg ctccacccaa cgtcgtcatc
     5641 cacaacccgg agttcgccaa gtgggctcgt tccgagcagg gcgacgccgc ggccatcgac
     5701 ggcgcgaaag ccctggctct gaccgccgcc gactacctcc tggatggctc gctgcaggcg
     5761 gaggtgaaca aagcgttcgc gatctcgaag ggcctggcct gaccgcccgc ccggcctgat
     5821 gaacgtctgg ctgttcggct acggatccct catctggcgc ccggagatcg cttacgtgga
     5881 gcgccgcccc gcccgcctcc acggctgggc gcgccgcttc tggcagggct cccacgacca
     5941 ccgcggcatg ccccgccagc ccggcagggt ggtgaccctc atcgaggagg aaggcggtca
     6001 ttgcgacggc atggcctatc tcgtggacag gggcgatgtc gaagccatat tcgcggcgct
     6061 ggatcatcga gagaagaacg gctatcggcg cttcgacgtg gaactgcatt tctctcgcta
     6121 caatcctgcg ccggccacca cgggagtggt ctacatcgcg cccccgggca atcacgcttt
     6181 cctgggaccc gccccgcttg cggagatggc tcgccagatc agcgagtgcg cggggccgag
     6241 cggccgcaac gtcgactacc tgctcgagct ggccgaagcg ctgcgtcagc ttgacgccca
     6301 ggacgcccat gtgttcgagt tggaagcggc cgtccggcac gtatccggga gcagataatg
     6361 gacatttcct tcagcgcgga gcacgaacgc ttccgcgacg aggttcgggt ttttctcgac
     6421 ggggccctgg acgacgagct gcgcgaagcc cagcgcttct gccccggcat cttcctggat
     6481 tacgagcaca acatccgctg gcaccgcatc ctgcacgcca agggctgggt cgcccccgcg
     6541 tggccccggg aatacggggg caccggctgg gacctgacgc gccggtacat ctggtccacg
     6601 gagaccaccc ttgccggcgc accctcgctc gcgccgatgg gccttggcat gtgcggcccg
     6661 atgctgatcg gctacggaac cgaagcccag aaagcttatt acctcccgcg gattctctcc
     6721 ggcgaggact actggtgcca gggctactcg gagccgggtt ccgggtcgga tctcgcctcc
     6781 ctcaagctcc gggcagacgg cgacggcgac cactacgtcc tgaacggaac caagatctgg
     6841 accacccacg cgcattacgc caatcgcatg ttctgcctcg tcagaaccga cgacggggga
     6901 cgtccccagc aggggatcac gtttctgctt ctcgacatgg acacgccggg catcagcgtg
     6961 gagcccatcg tgttcgcatc gggcactcac gaggtcaatc aggtgttctt cgacgatgtc
     7021 agggttccca aggccaaccg tgtcggtgag gagaacgacg gctggaccgt ggccaagtac
     7081 ctgctcgagt tcgaacgagg tggcggcggc agcgccgcct accggacggc cctgacccgg
     7141 gttcgcaaac tggccggcca ggttcccgcc gatggcgcgt ccctggccga agacccggat
     7201 ttcaagcggg cgctggacga aaccgacgta aagctgcagg ccattcaata caccgagttt
     7261 cgcatcatgg ccgcgctctc ccggggccag aaccccgggc ccgaatccac cataatgaag
     7321 aacctgggcg ccgacatcgg ccagcgcctc accgagctga ccgtcgaagc catgggccag
     7381 tacatcgcgc cccatcagcc cgaggcccgg caggtgggca gcaacgtccc cgtggtagga
     7441 ccccc
//
LOCUS       DBNHQR010000489         1847 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1315547, whole genome shotgun sequence.
ACCESSION   DBNHQR010000489 DBNHQR010000000
VERSION     DBNHQR010000489.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1315547"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="AC16VR_11045"
     CDS             <1..343
                     /locus_tag="AC16VR_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011737.1"
                     /translation="SSYEAVYQLQTYAGIKFQEELVEQLTQAIGVFPTGTLVELNTGE
                     VGVVIAQNPERRLRPKINLILNEEKNLRRLPIITDLNAMDTDPATPPRVWIKRELPHG
                     AYGIEATDYFI"
     gene            complement(400..1263)
                     /locus_tag="AC16VR_11050"
     CDS             complement(400..1263)
                     /locus_tag="AC16VR_11050"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA dioxygenase family protein"
                     /protein_id="HIR6011738.1"
                     /translation="MSRSTFDVRPLDASFGAVVTGLRLADLGDDAFFDLYDAWLQHAL
                     LIFPDQHLSNAEQVRFAQRFGALEFDLTPLSNVRGDGSLRPDDGSDDVVKVLRGNMGW
                     HCDSTYMPVQAKGAVFTAHVVPDEGGETGWADMRAAYDALDEEMRERIADLCAYHSLY
                     HSQAKVGHVPSSESAYSNYGFHEGEPPLRPLVKIHPETGRPALMVGRHAYGIPGMDAD
                     ASERLLDELVEFACRPPRIYHHTWSPGDAVVWDNRCLMHRACPWDMSKPRVMYHARIA
                     GDPASESGLAA"
     gene            complement(1309..>1847)
                     /locus_tag="AC16VR_11055"
     CDS             complement(1309..>1847)
                     /locus_tag="AC16VR_11055"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024802.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6011739.1"
                     /translation="DFSPAYRATPEWRRLNPVGKVPVLVDDDVTMFESGAMVEHLLDR
                     HGAGRLRPPPGTAERAAYLQWSWFAEATFARPLGEIVNHRREFPGESEIPAVLEEMSA
                     RAALCVQALDEALAGRQYLLGDEFTAADVMMGYSVLLARALGIEGARAGRVGAYFDVL
                     ARRSGYPAATADLPDAWR"
BASE COUNT          353 a          621 c          573 g          300 t
ORIGIN      
        1 gtcctcctac gaggccgtct atcagctgca gacgtatgcc ggcatcaaat tccaggagga
       61 gctcgtcgag cagctgaccc aggccatcgg cgtgtttccc acgggcacgc tcgtggagct
      121 caacacgggc gaagtgggcg tggtcatcgc gcagaacccc gagcggcgtc tgcgacccaa
      181 gatcaacctc attctgaacg aggagaagaa cctgcgccgc ctgcccatca tcaccgacct
      241 caacgccatg gacaccgatc ccgccacgcc ccccagggtc tggatcaagc gggagttgcc
      301 tcacggcgcg tacggaatcg aagccaccga ctacttcatc tgagggcagg tctcgatgcg
      361 cttgacgccg atcttgcgag cggggggcag agcgaccgct cacgcggcca gtccggactc
      421 cgaggcaggg tcgccggcaa tacgggcgtg atacatcacc cgcggcttgc tcatgtccca
      481 cggacacgcc cgatgcatca gacagcggtt gtcccacacc accgcatcac ccggcgacca
      541 cgtgtggtga tagatgcgcg gcgggcggca ggcaaactcg acgagctcgt caagcagacg
      601 ctctgaagca tcggcatcca tgcccggaat gccgtaggca tggcggccca ccatgagagc
      661 cggccggcct gtctccgggt gaatcttcac gagagggcgc agaggcggct cgccctcgtg
      721 aaacccgtag ttgctgtagg cgctttcgct gcttggcaca tgacccacct tcgcctggct
      781 atggtagagc gagtggtagg cacacaggtc ggcaatgcgc tccctcatct cctcgtccag
      841 cgcatcgtaa gcggcacgca tgtccgccca gcccgtctcg ccgccttcgt ccgggacgac
      901 gtgggcggtg aacaccgcgc cttttgcctg gacgggcatg taggtgctgt cgcaatgcca
      961 gcccatgttg ccgcgaagta ccttgaccac gtcatcgctg ccgtcatcgg ggcgcaggct
     1021 gccgtccccg cgcacgttgc tcagcggcgt gagatcgaac tccagcgctc cgaaccgctg
     1081 cgcaaatcgg acctgctcgg cgttgctcag gtgctgatcg gggaagatga gcagcgcgtg
     1141 ctgcagccat gcgtcataaa ggtcgaaaaa agcatcatcc ccaaggtctg caaggcgcaa
     1201 gccggtcacc acggcgccga acgaggcgtc cagcggccga acatcaaatg tcgatctgct
     1261 catgcttttc ctccggttct tcatcgctca ttccggggcg cggcagcgtc agcgccaggc
     1321 gtcgggaagg tccgccgttg ccgcaggata tccggacctt cgggccagca cgtcgaagta
     1381 tgcgcccaca cgaccggccc gggccccctc aattcccaga gcacgcgcca gcagaacgga
     1441 atagcccatc atcacgtccg ccgccgtgaa ctcgtcgcca agcagatact ggcggccggc
     1501 gagggcttcg tccagcgcct gaacacagag ggcagcacgg gcgctcatct cctccaacac
     1561 agccggaatt tctgattcgc cgggaaactc ccggcgatgg ttgacgatct cgccgagcgg
     1621 ccgggcaaag gtggcttccg caaaccagga ccattgcaga taggcggcac gctcggcagt
     1681 acccggcggg ggacgcagcc gacccgctcc gtgacggtcg agcagatgct cgaccatggc
     1741 accggattcg aacatggtga cgtcgtcgtc gacgagcacg ggcactttgc ctaccggatt
     1801 gagccttcgc cattcggggg ttgcccggta ggccggagag aagtcga
//
LOCUS       DBNHQR010000490         3302 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_301550, whole genome shotgun sequence.
ACCESSION   DBNHQR010000490 DBNHQR010000000
VERSION     DBNHQR010000490.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_301550"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            260..1498
                     /locus_tag="AC16VR_11060"
     CDS             260..1498
                     /locus_tag="AC16VR_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017642823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR6011740.1"
                     /translation="MSNKPRLHIPHPPARPGQEPDFSYVDISPAGAVPRPDVTTRLRD
                     IERSLAFEMVRVLDDDHAAVGSWNPHLDDHDLQIGLRHMLLTRLYDDRMLRMQRQGRI
                     SFYIKCTGEEAVAIAPAMALRPSDMLFPSYRTQGLQIARGRSLVDLMCHCLSNSRDMC
                     KGRQMPVMYHWARGNIFSVSGNLTTQFPQAVGWAMAAAIKGDDDVAAAWIGEGSSAEA
                     DFHHAMTFASVYLAPVILNIVNNQWAISTFQGFAGGERRPFSARGPGYGIPGIRVDGN
                     DFLAVHSVTSWAAERARRGVGPTLIEHVTYRASAHSTSDDPSRYRPRDEERSWPLGDP
                     IERLKQHMIREGYWSQAQHEQLVEELQAAVDEAWQEALSYGSMTEGPWLDRSEMFEDV
                     FKDVPAHLLAQREQLRALEE"
     gene            1512..2528
                     /locus_tag="AC16VR_11065"
     CDS             1512..2528
                     /locus_tag="AC16VR_11065"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010955111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR6011741.1"
                     /translation="MAQMNMIQALNSALNIMLSRDPGVIVIGQDVGFFGGVFRVTDGL
                     QRQYGEHRVIDTPIAEGGIVGSAIGMGVNGLRPVAEIQFADYIYPGCDQIISELARLR
                     YRTGGDFFAPVTIRTPCGGGIRGGQTHSQSPEGIFTHVCGLKVVMPSNPYDAKGLLIS
                     AIEDDDPVIFFEPKRIYNGPFDGDANKPAVPWSTHPKGEVPEDYYTIPIGRAEVIRPG
                     SEVTILCYGTMVHVCAAAAEAAEIDAEIIDVRTMAPLDVETLKTSVEKTGRCVIAHEA
                     TRFSGYGAELAATVSEECFYHLEAPIQRVAGWDTPYPHAFEWEYFPGQKRVAAALERV
                     LEAP"
     gene            2525..>3302
                     /locus_tag="AC16VR_11070"
     CDS             2525..>3302
                     /locus_tag="AC16VR_11070"
                     /inference="COORDINATES: protein motif:HMM:NF012584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HIR6011742.1"
                     /translation="MSEYVFKLPDLGEGTVEAEIVEWHVRPGDEVNEGDLLVDVMTEK
                     ASVEVPAPVTGTVLRTSGMPGDLIAVGAELAAIETGASQETTKAPSTVGSAIALPESP
                     DVSMRPEPVEAPIEDAVTHPEPSSPSSADSDQVATLPDTPVESARRVITSPAIRRRAK
                     EAGVDLESLTGTGPRGRILQRDLEQAIAGRETAAPPASGTGAVNEVKVIGVRRVIAKR
                     LQAAVREIPHFTYVEEVDVTALESLRSHLNADRAEGRPALT"
BASE COUNT          656 a         1078 c         1033 g          535 t
ORIGIN      
        1 ccgcccgccg actctgaatc tttagccaag tcgcctcctc cgcccattgg tagcataggc
       61 aatcgcccca ggcctgaaca gtagtagaat catgaggggg caacacggac gtaacccgtc
      121 atcatgcggt caatcgcgac gcggaaattc tgggtagcgc cggcgcagcg caaagcatcg
      181 ccgagggcga tggcctgcac ccgatcatga cgtcacagcc catcatgcac acggatagaa
      241 gccccggagg ccttagacaa tgagcaacaa acccaggctg cacataccgc acccgccggc
      301 tcggccgggg caggaaccag acttcagcta cgtcgacatt tcccctgcag gcgcagtgcc
      361 ccggcccgac gtcactaccc ggctcagaga catagagcgc agcctcgctt tcgagatggt
      421 acgcgttctg gacgacgacc acgccgccgt cggctcctgg aatccgcatc tggacgacca
      481 cgatcttcag atcggcctgc gacacatgct tctcacacgc ctgtacgacg accggatgct
      541 gcgcatgcag aggcagggtc gcatttcgtt ctacatcaag tgcaccggag aggaagccgt
      601 cgccatcgcc ccggcgatgg ccttgaggcc gagcgacatg ctgttccctt cctaccgcac
      661 tcaaggcctg caaatcgcca ggggccgcag cctggtcgat ctcatgtgcc attgcctgtc
      721 gaacagccgt gacatgtgca aaggtcgaca gatgcccgtg atgtatcact gggcccgagg
      781 gaacatcttc tcggtttccg gcaacctgac cacccagttc cctcaggccg tgggctgggc
      841 catggccgcc gccatcaagg gcgacgacga tgtggccgcc gcatggatcg gcgagggctc
      901 aagcgccgag gcggactttc accatgcgat gacgttcgca tcggtctacc tggcccccgt
      961 catcctcaac atcgtgaaca accaatgggc aatctcgact tttcagggtt tcgcgggcgg
     1021 ggaacgccgc cccttctcgg ccagggggcc cggttacggc attccgggaa tccgggtaga
     1081 cgggaacgat tttctcgccg tgcactcggt aacgagctgg gccgccgaac gcgcacgacg
     1141 cggtgtggga ccgacgctca tcgagcacgt gacctatcgg gcatccgccc actccaccag
     1201 cgacgacccg tcgcggtatc gtccccgcga cgaagaacgg agctggccgc tgggagatcc
     1261 catagagcgt ctgaaacagc acatgattcg cgaaggttac tggtcacagg cccagcacga
     1321 gcagctggta gaggagttgc aggccgctgt cgacgaggcc tggcaggaag ccctgagcta
     1381 cggctccatg acggagggtc cgtggctcga ccgcagcgag atgttcgagg acgttttcaa
     1441 ggatgttccc gcccacctgc tcgcccagcg cgaacagctc cgcgcgctgg aagaatgagg
     1501 aggtcaccga catggcccag atgaacatga tccaggcgct aaactcggct ctgaacatca
     1561 tgctgagtcg agacccgggc gtgatcgtca ttggccagga cgtcggtttc ttcggcggcg
     1621 ttttccgggt caccgacggt ctgcagaggc agtacggcga gcaccgcgtc atcgatacgc
     1681 ccatcgccga aggaggcatc gtgggcagcg ccatcggcat gggcgtcaac ggactacggc
     1741 cggtagccga gattcagttt gccgactaca tctacccggg ttgtgatcag atcatcagcg
     1801 agctcgcgcg cctgcggtat cgaaccggcg gcgacttctt cgccccggtg acgatcagaa
     1861 cgccctgcgg aggcggcatt cgcggcgggc agacccactc ccagagtccg gagggaatct
     1921 tcacccacgt ctgcgggctg aaggtcgtca tgccctcaaa cccttacgat gccaaggggc
     1981 tcctgatcag cgccatcgag gacgacgacc cggtgatttt cttcgagccc aaacgaatct
     2041 acaacggccc cttcgacggc gacgcgaaca aaccggccgt tccgtggagc acgcacccca
     2101 agggcgaggt gccggaagac tactacacga tccccatcgg ccgcgccgag gtgatacggc
     2161 ccggcagcga agtgaccatc ctctgttacg gcaccatggt tcacgtctgc gcggcggccg
     2221 cggaagccgc tgaaatcgac gccgagatca tcgacgtgcg gaccatggcg ccgctggatg
     2281 tcgaaacgct caagacctcg gtggagaaga cggggcgctg cgtaatcgcc cacgaagcaa
     2341 cgcggttcag cgggtacggc gccgagctag ccgctacggt tagcgaagag tgcttttacc
     2401 acctcgaagc acccatccag cgagttgccg gttgggatac cccgtatccc catgcattcg
     2461 agtgggagta ttttccgggc cagaagcgcg tcgcggccgc cctggaacgg gtactggagg
     2521 cgccatgagc gaatacgtat tcaagcttcc cgatctcggc gagggaacgg tcgaggcgga
     2581 gatcgtcgag tggcatgtac gcccgggcga tgaagtgaac gaaggcgatc ttctggttga
     2641 cgtgatgacc gagaaagcca gcgtggaggt ccccgcgccg gtcaccggga ccgtgctcag
     2701 aacgtccgga atgccgggcg atttgatcgc cgtcggcgct gagctggccg ccatcgagac
     2761 cggagcctcc caggagacga cgaaggcccc gagcacggta gggtccgcca tcgcccttcc
     2821 ggaatcccct gatgtctcca tgcggcccga gccagtggag gcacctatcg aggacgcggt
     2881 cactcatcca gaaccctcgt ccccgtcgtc ggccgattca gaccaagttg ccacgctgcc
     2941 cgatacgccg gtggagagcg caaggcgcgt gatcacgtcc cccgccatcc gacgacgggc
     3001 gaaggaggcc ggcgtcgatc tggagtcgct cacgggcacg ggaccgcgcg gacggatact
     3061 gcagcgcgat ctcgagcagg ccatcgccgg ccgggagaca gcagcgcccc cggcgagcgg
     3121 cacgggtgcc gtaaacgaag tcaaggtcat cggcgtgcgt cgcgtcatcg ccaagcggct
     3181 tcaggcagcc gtgcgcgaaa ttccgcactt cacctacgtc gaggaagtgg atgtcaccgc
     3241 cctggaatcc cttcggtcgc acctgaacgc tgatcgggca gagggtcgcc ctgccctgac
     3301 ct
//
LOCUS       DBNHQR010000491        15015 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_301730, whole genome shotgun sequence.
ACCESSION   DBNHQR010000491 DBNHQR010000000
VERSION     DBNHQR010000491.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 15015)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15015)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15015
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_301730"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..1733
                     /locus_tag="AC16VR_11075"
     CDS             3..1733
                     /locus_tag="AC16VR_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012271558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6011743.1"
                     /translation="MLLAGCDGSAPDAPGVDRHAAAKRWITQEFQPSTLTPDEQAAEM
                     AWFIEAAAPFRGMEISVVSETLTTHEYEAGVLARAFAEITGIRVSHDLIQEGDVIEKL
                     QTQMQSGRNVYDAYVNDSDLIGTHARYGYVVALSDFMAGEGADVTLPTLDVDDFIGRS
                     FTTGPDGKLYQLPDQQFANLYWFRYDWFQREDLRARFRELYGYDLGVPVNWSAYEDIA
                     EFFSEYVRTIDDQRIYGHMDYGKKDPSLGWRFTDAWLSMAGAGDPGIPNGLPVDEWGI
                     RVDGCRPVGASVSRGGAANGPAAVYALEKYIDWLKRFAPPQAPGMTFSEAGPVPAQGH
                     IAQQIFWYTTFTADMIDGDLAVVDEDGLPRWRMAPSPHGPYWEEGMKLGYQDAGAWTL
                     LASTPLERRKAAWLYAQFVVSKTVSLKKTLVGLTPIRDSDLRSAAMTEAAPRLGGRVE
                     FYRSPARTLWTPTGTNVPDYPKLAQLWWPNVANAVSGAVSAQEAMDTLARDQDRVLER
                     LQRAGIQGDCGPELNPEVDPEEWLARPGAPKPRLADEKPPGKTVPYDELVADWGALPR
                     PGEPIGRRSH"
     gene            1737..2654
                     /locus_tag="AC16VR_11080"
     CDS             1737..2654
                     /locus_tag="AC16VR_11080"
                     /inference="COORDINATES: protein motif:HMM:NF018649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HIR6011744.1"
                     /translation="MAQPTQTARGGPGRGSAFGWIWIAYLAAVVVGVAGTMIVADPAT
                     DHPVYLAGIADVLATVVIFAFSVAFRNSSFYDPYWSVVPPVLGLYWWWVVGSVDDPRV
                     WLAMLVVSCWAVRLTYNWAFGWTGLGHEDWRYRDLQNRLGILYWPVSFLGIHMMPTVL
                     VFLGCVPLFLIMGRESGPLNGIDLLALAVGAAAVWIETRADLELHRFRARRGSPAEAL
                     TTGVWGWCRHPNYLGEIGLWLSLGLFAWAAGADPVWCFAGFVAMVALFELVSIPMIEK
                     KLLAAKPGYEAYRRSTFRLLPFSALKRFS"
     gene            2677..3396
                     /locus_tag="AC16VR_11085"
     CDS             2677..3396
                     /locus_tag="AC16VR_11085"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6011745.1"
                     /translation="MLIHGAWHGGWCWHKLVAELSRRGHRALAPDLPAHGHDATPRDR
                     VTLDAYADSVIEHLNDFAGPVCLVGHSMGGMVISAVGQRVPERLERLVYLCAFVPADG
                     MSLLSSDPTESGAQAFAGKIRGGEDGASVMIDDAAVAALFYHDCTDEDVAFARARLVP
                     QPSAPLGAAVALTDAAFGSLAKSYIECTADQAAPIAAQRAMYTRAGIDSVVSLSTSHS
                     PFFSAPTLLADSLEALIGTPA"
     gene            3509..5191
                     /locus_tag="AC16VR_11090"
     CDS             3509..5191
                     /locus_tag="AC16VR_11090"
                     /inference="COORDINATES: protein motif:HMM:NF021354.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPL-N domain-containing protein"
                     /protein_id="HIR6011746.1"
                     /translation="MISSRRGGYAAFFATIVVALTLGASPADGARGGKKGGGGPVCKI
                     TDQTDVAIYVGGGIGPSSQLWAEALIDFWKTGSRIPGEPGLLNDDGGATWTGDGSLDY
                     VTLTLSEFNACRDDDFAKLELFFMPGGSAYEIQANLGTSGKDRLTRFLDSGGSYVGMC
                     AGGYYAARGYYWKGDDGAPTDNCRNQFCRYEVDGTFSYEAATSDFTVHEWGGVSYHAD
                     LLAYGPLAEVLVEGPIEEIAGPWHAQSDPNHPYDSHLVTTDDPAMPWLRAVYWGGATE
                     NYVYTDNAPWGTEHAHFAADASGNDDLPFPTDGSLWTLKSVATGTGGAIMISSAHLEA
                     SLFHTASTFTDGGMTECQQYNNYTYLIRRMQSQLGAPFAVPGYDAFCAPDRAGEVKET
                     AVLFPSGLAYQNAPRIDTSGGGGGDGGGDGGGGTGSTSISFDDGTLGDFQLEGDGLRP
                     WQANATAACNGAYGAMAGVPGGVDMESFLTLTVPAGVSRVTYRYSYPSALDRGDDFHV
                     IVNGAVRKSYETGSGAQCATDSLPVTPGDELRFKCRSGGNGETCAVDEIVFD"
     gene            complement(5216..6793)
                     /locus_tag="AC16VR_11095"
     CDS             complement(5216..6793)
                     /locus_tag="AC16VR_11095"
                     /inference="COORDINATES: protein motif:HMM:NF013206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HIR6011747.1"
                     /translation="MTTQAGGAGVPARGGLGAVACGHPATACAAAEILEEGGNAFDAV
                     IAAQFAACVAEPVLASVGGGGFLLAHPAPGAPVVYDFFVQTPRSPREASAVDFRPVHA
                     DFGTATQEFHIGLGAAATPGSLAGMEAVHGDLGSLPMARLVEPAARAAREGVRIERLQ
                     ASIFEIVKPIYAASSEARSIYGGIQEGRLLRQSDLADTLEQVARQGAGRFYRGAFADA
                     VARLAQEQGGHLSMEDLLGYGVVKRMPLSLRHAGATVLTNPPPSSGGILIGFGLQLLQ
                     AMAVADQTFGGPSHLAALAEIMAATNQARVELLAAAGSHAELDPALVERYRREVLGTP
                     RFNRGTTHISVVDAKGNAAAMTLSNGEGCGTMIPGTGVMLNNMLGEEDINPDGFGNWP
                     PDQRLTSMMAPTLVETVAGRRLALGSGGSNRIRTAIVQVISNLLDFGDSVDAAVRRPR
                     LHLEGDLLSIEDGFDRDAVRALEAGPAEIRRWPGQSLFFGGVHVAELAKDLFRAAGDP
                     RRGGVGRVVTARGAPEG"
     gene            complement(6806..7852)
                     /locus_tag="AC16VR_11100"
     CDS             complement(6806..7852)
                     /locus_tag="AC16VR_11100"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6011748.1"
                     /translation="MALALEEHTATREGHTTFYLAAGPEDGPLIIFVHGWPELSLSWR
                     HQLPALAALGYRAVAPDMRGYGRSSTYERYEDYAQRAVVGDMMALLEGLGRTEAIWVG
                     HDWGAPTVWSIASHHPEACTAVAGLCVPYFTLERGLQACVDQVNRDVYGPEYPAGQWE
                     YQLFYQEHFDRARAEMDANPLNTVKLLFRRGDPNGAGRPAATAMTRINGGWFGPAGEA
                     PDVPRDDDVISEEDLAVYADALARNGFFGPNAYYMNHAENSAYAAEAVNDGYLDMPVL
                     FLGARYDYVCECYDSTLADPMRERCRRLTETTVYSGHWMAQERPLDVNAALVRWLATD
                     LPEVWPRSRAPDPA"
     gene            7983..8585
                     /locus_tag="AC16VR_11105"
     CDS             7983..8585
                     /locus_tag="AC16VR_11105"
                     /inference="COORDINATES: protein motif:HMM:NF013460.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HIR6011749.1"
                     /translation="MRIWHWVLAACVTGSLYTGLAGEIALMERHQQFGYGVLGLLLFR
                     LGWGLWGGRYARFGNYRLAPRRLIEHFRGLDHGAAHTAPGMAFTIVFLLALAAQVGTG
                     LFATDDIFTQGPLSRLVENDTARALTGIHHRVYWLILAALAAHLTAHAVYALRRDPMP
                     LAMITGRKRLDLEPARAAWTAALLTALGAAALVWAMLELA"
     gene            complement(8589..9017)
                     /locus_tag="AC16VR_11110"
     CDS             complement(8589..9017)
                     /locus_tag="AC16VR_11110"
                     /inference="COORDINATES: protein motif:HMM:NF013487.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6011750.1"
                     /translation="MRGRDWLIGLVALGAASAATASEGELEYREHTMEAIGGHMQAAV
                     DILRQKVPHASHMTIHADALAALSGIVGTLFPEGSEGGDALPAIWEEPEDFAGRVEAM
                     REAATAFSAAARSNDDAALGQAFQALGQSCKGCHDNYRAD"
     gene            9148..9591
                     /locus_tag="AC16VR_11115"
     CDS             9148..9591
                     /locus_tag="AC16VR_11115"
                     /inference="COORDINATES: protein motif:HMM:NF015180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerC domain-containing protein"
                     /protein_id="HIR6011751.1"
                     /translation="MIVYATMPNAPSPLPEAHPAVQPVPNGMDRFAITLSGLCLLHCL
                     LIPVVLAMAPAFSSLVLGTETTVHWVFLGLAVPTSVWALWRGYGRCQHLRALVLGIAG
                     LLVMFLGVSHLFDPRLEVPLTLVGVTMVVVAHVLNLRIPEPAPRG"
     gene            9655..10140
                     /locus_tag="AC16VR_11120"
     CDS             9655..10140
                     /locus_tag="AC16VR_11120"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012075279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:9760..9762,aa:Sec)
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HIR6011752.1"
                     /translation="MSTFHDIRMESIDGEPVELSKFQNNLCLIVNVASRUGLTPQYAG
                     LRTLQEQYGDRGFTVLAFPCNQFGAQEPGSNEEILDFASSKFDVNFPMFGKIEVNGSG
                     ACELYQYLKAARADEEGNADIAWNFTKFLVDGDGNVIARFAPPVTPEEIGEQIGSLLP
                     G"
     gene            complement(10178..11209)
                     /locus_tag="AC16VR_11125"
     CDS             complement(10178..11209)
                     /locus_tag="AC16VR_11125"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR6011753.1"
                     /translation="MSADYRLVEVAEGLELPWCVAFLPDGRLLVTERPGRLRQISPEG
                     EIGSPMEGIPEVLFRAQGGLFDVAPHPDFAENRLIYISYAHGTPRANTTRIARGRLDG
                     NSLKDLEVIFTARPNKTTPQHYGGKFLFLPDGTLLFTTGDGFDHREEAQDLSGLLGKT
                     VRIRDDGSIPDGNPYVRREGADPAVWTWGHRNPQGLAVDHVTGRVFLHEHGPRGGDEI
                     NLLRPGRNYGWPVITFGLDYSGARISPYQQMPGMEQPLHSWVPSIAPSGLAVYRGDVF
                     PEWQGDLFVGALVDREARRVRLRDDRVVEEEALFGELGERLRDIRVGPEGHLYLVTDE
                     GRVLRVERP"
     gene            11339..11788
                     /locus_tag="AC16VR_11130"
     CDS             11339..11788
                     /locus_tag="AC16VR_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011754.1"
                     /translation="MKPLAATVIARIAGAYLLSVLVAYLLAATAATQASLAGLEGLGV
                     TITVGERISTTGQDLMGMLPTYGLLIAIGFAIALPIAAAFARFIPSWRAVGLVAAGAA
                     AVGLIHVGLNSVFETHLVAATRTTVGLLVQVMAGGVGGYAFYRLRDP"
     gene            11864..13420
                     /locus_tag="AC16VR_11135"
     CDS             11864..13420
                     /locus_tag="AC16VR_11135"
                     /inference="COORDINATES: protein motif:HMM:NF015746.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HIR6011755.1"
                     /translation="MREPHSSASGRGPAAMGRFLDAVERAGNRLPDPALLFFLLLLAV
                     WALSALLSQLDYGHVDPRSGDPVEVRNLLTGEALTSFFAGMVETFVGFHPLGVVLLAM
                     LGIGVADHTGFIKAGLRRLLSLTSQRLLTPMLFLIGILSHSAVDAGYVLVIPLGGVIF
                     QAAGRHPLAGIAVAFAGVSGGFSANFLPSALDPMLQGISQAGAQLMDPGLVLNPLNNW
                     FFTSASTLLVVLLGWYLTARIVEPRLADEPLDGDLADAPALDDLSDEERRGLRAGLVA
                     MLLGIALLAVTAWPDSSAWRSGGQLTAAGAPLMQSIVPLIFLLFIIPGVVYGYVSGTV
                     RGHRDVIAGMSQAMSGMGYYIVMAFFASLFIAEFGRSNLGVLVALQGAAWLEAAAMPA
                     AVTVVGVVAITGVVNLFVGSASAKWALLAPVLVPMLMALGISPDLAQAAYRVGDSSTN
                     IITPLMPYFPLVVVFAQRYVTRTGIGTIVAMMLPYSVGFLVVWTLFLLVYWMLGIPLG
                     LQASYGYPGG"
     gene            13506..14360
                     /locus_tag="AC16VR_11140"
     CDS             13506..14360
                     /locus_tag="AC16VR_11140"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR6011756.1"
                     /translation="MSLFRIALRVIVLLALPVWAVAGTLDEIAASGEFRLAYRADTPP
                     FSSRGKGGEPEGFTIDLCRIIARDVQAALNLDEMKITWVPVTAENRFDAIVSGRAHLE
                     CGSSTVTLSRQERVDFSNLTYVTGASLMRWADSDIDRVGDLDGRRVSAIAGTTTEKVL
                     KETLERDGIDAKVVVVGDHAEALQLLLDRKIDAMAGDQATLFGIGFKTQGDDDLVITE
                     DLLSFEPYALPLRRNDADFRLAVNRSLSELYVRGDVGRSWEKWFGSHGVRPTRLLLML
                     YRLNSFSE"
     gene            complement(14401..>15015)
                     /locus_tag="AC16VR_11145"
     CDS             complement(14401..>15015)
                     /locus_tag="AC16VR_11145"
                     /inference="COORDINATES: protein motif:HMM:NF013954.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HIR6011757.1"
                     /translation="LVAADRARLLELAKGPGQLAPDDADWLANLAARYRLRDESAAGP
                     EAIEELMLRVDVLPPSLILAQAAEESGWGTSRFADVGNALFGQWAWDDGIIPLERREE
                     LGEYYVARFETPLDSIRAYMRNLNTHRAYRRLRARRAELRAMGELPSGWELARTLTSY
                     SERGADYVRTLHSIMKANNLDAVDDAYLRDMEPIALVPVGDGAH"
BASE COUNT         2318 a         4911 c         5034 g         2752 t
ORIGIN      
        1 ctttgctgct cgccggttgc gatgggtccg cgccggacgc gcccggggtt gaccggcacg
       61 cggcggcgaa gcgctggata acccaggagt ttcaaccctc cacgctcacg ccagacgagc
      121 aggcggcgga aatggcgtgg ttcattgagg ctgccgcgcc gttcaggggc atggaaatca
      181 gcgtcgtctc ggaaaccctg accactcacg agtatgaagc gggggtgctc gctcgggcct
      241 tcgccgagat cacgggtatc cgggtgtccc acgatctcat tcaggaaggc gacgtcatcg
      301 agaagctgca gacccagatg cagtccggcc gcaacgtcta cgacgcctac gtcaacgact
      361 ccgacctcat cggcacccac gcgcgttacg gttacgtggt cgcgctgtcc gacttcatgg
      421 ccggcgaggg cgccgacgtc acccttccca ccctggacgt ggacgatttc atcggccgga
      481 gctttaccac gggtccggat ggcaagctct accagctgcc ggaccagcag ttcgccaacc
      541 tctactggtt tcgatacgac tggttccagc gcgaggatct tcgtgcccga ttccgcgagc
      601 tctacggcta cgacctcggc gtgcccgtga actggtcggc atacgaggac atcgcggagt
      661 tcttctccga atacgtccgg accatcgacg accagcggat ctacggccac atggattacg
      721 ggaagaagga cccgtcgctg ggctggcgct tcaccgatgc ctggctgtcc atggccggtg
      781 ccggagaccc ggggatcccg aacggcctgc ccgtggacga atggggaatc cgggtggacg
      841 gctgccgtcc ggtcggcgcc tccgtcagtc gcggcggggc ggccaacggg cccgcggccg
      901 tctacgcgct ggagaagtac atcgattggc tgaagcgctt cgcgcccccg caggccccgg
      961 ggatgacttt ctccgaagcc gggccggtgc cggcccaggg tcacatcgcc cagcagattt
     1021 tctggtacac gaccttcacc gcggacatga tcgacggcga cctggccgtg gtcgacgaag
     1081 acggcctgcc gcgatggcga atggcgccgt ccccccatgg accatactgg gaggaaggca
     1141 tgaagctcgg ttatcaggat gccggcgcct ggacgctgct tgccagcacg ccgctggagc
     1201 gtaggaaggc tgcctggctg tacgcgcagt tcgtggtttc caagaccgtc tccctcaaga
     1261 aaaccctggt ggggctgacg cccattcgtg attccgacct gcggtcggcg gccatgaccg
     1321 aggccgcgcc gcgtctgggc gggcgggtgg agttctacag gagcccggcg cggacgctct
     1381 ggacgcccac ggggacgaac gtgcccgact atccgaagct ggcacagctg tggtggccca
     1441 acgtggccaa tgcggtgagc ggcgccgtca gcgctcagga ggccatggat accctggcgc
     1501 gggatcagga cagagtgctg gagcgccttc agcgggcggg catccagggc gattgcggac
     1561 cggagctgaa tccggaggtg gatcccgaag agtggctcgc acggcccggt gcgcccaagc
     1621 cccgcctggc cgacgagaag ccgcccggca agacggttcc ctacgatgag ctggtggcag
     1681 actggggggc tttgccgcgg ccgggcgagc cgatcggccg aaggagtcac tgaaacatgg
     1741 cacaaccaac acagactgcc aggggcggcc cgggccgggg cagcgcgttt ggctggatct
     1801 ggatcgccta tctggcagct gtcgtggtgg gtgtggccgg cacgatgatc gttgccgacc
     1861 cggccaccga tcaccccgtt tatctggccg gcattgccga cgttctggcc accgtcgtga
     1921 tcttcgcatt cagcgtcgct ttccgaaatt ccagtttcta cgatccgtac tggagcgtgg
     1981 tgccaccggt gctgggcctt tactggtggt gggttgtggg ctccgtagac gatccgcggg
     2041 tctggcttgc catgctcgtc gtcagctgct gggcggtacg cctgacctac aactgggctt
     2101 tcggctggac gggccttggc cacgaagact ggcggtatcg cgacctgcag aacaggctgg
     2161 gaatcctcta ctggcccgtg agcttccttg ggatacacat gatgcccacg gtgctggtct
     2221 tcctcgggtg cgtgccgctt ttcctgatca tgggtcggga atcggggccc ctgaacggca
     2281 tcgacctgct ggccctggcg gtgggggccg cggcggtctg gatcgaaacc cgcgccgatc
     2341 tcgagctgca tcgctttcgg gcccgtcgcg gctcacctgc cgaggccctc acaaccgggg
     2401 tctggggttg gtgccgccat cccaactatc tcggcgagat cggcctgtgg ctctcgctcg
     2461 ggctgttcgc ctgggcagcc ggggcggatc cggtctggtg cttcgccggc ttcgtcgcca
     2521 tggtggcgct cttcgagctc gtgagcatcc cgatgatcga gaagaagctg ctggccgcca
     2581 aacccggtta cgaggcgtat cggcggtcca cgttccgcct gctgcctttt tccgcgctca
     2641 aacgtttctc ctagagggcc gatcatggcg aacttcatgc tgattcacgg tgcctggcac
     2701 ggaggctggt gctggcacaa gttggtcgcc gagctcagcc gccgggggca ccgggccctc
     2761 gcgcccgatc tgccggctca tgggcacgat gccacgcccc gcgaccgggt gacgctggac
     2821 gcttatgccg acagcgttat cgagcatctc aatgactttg caggccccgt ctgcctggtc
     2881 ggccactcca tggggggcat ggtgatctcg gcggtcggtc agcgggttcc cgagcggctc
     2941 gagcggctcg tctacctgtg cgcgttcgtg cctgccgacg ggatgtctct gctgtcctcg
     3001 gaccccacgg agagcggagc ccaggctttt gccggaaaga tccgcggggg tgaagacggt
     3061 gcctcggtga tgatcgatga cgccgccgtg gccgcattgt tctatcacga ttgcaccgac
     3121 gaggacgtgg ctttcgcgag agcccgcctg gttcctcagc ccagcgctcc gctgggtgcg
     3181 gccgtcgccc tgactgacgc ggctttcggc agcctggcca agagctacat cgagtgcacg
     3241 gcggaccagg ccgcgcctat cgctgcccag agggccatgt atacccgcgc cgggatcgac
     3301 tcggtggtca gcctaagcac gtcccactcg cccttcttct cggcccccac cctgctcgct
     3361 gactccctcg aggcgctcat cgggacgccg gcatgaggcc tcgggagtgc aattggctta
     3421 cgaatcggta acaagtcgtc gttgcgtcga gcatgcacgg gtcacagact ggcgtgaagg
     3481 acgggaagga atacatatgc aacgggtcat gatcagctcg cggcgtggtg ggtatgccgc
     3541 gtttttcgca acgatcgtcg tcgcgctcac gttgggcgcc tcgcctgccg atggggcgag
     3601 gggcggcaag aagggcggcg gtggaccggt ctgcaagatc accgaccaga cggacgttgc
     3661 gatttacgtt ggcggcggca tagggcccag cagccagctg tgggccgagg cgctgatcga
     3721 cttctggaaa accgggagcc gcatccccgg tgagcctggg cttctcaacg acgacggcgg
     3781 ggccacctgg acgggcgatg gcagcctgga ctacgtcacc ctcacgctgt cggaattcaa
     3841 cgcctgcagg gacgacgact tcgccaagct ggagcttttc ttcatgccgg gcgggagcgc
     3901 ctacgagata caggcgaacc tgggcacttc cggcaaggat cggctgacgc gctttctgga
     3961 cagcggcggc agctatgtcg gcatgtgtgc cggcggctac tacgcggcgc gcgggtacta
     4021 ctggaaaggt gacgacggcg ctcccacgga caattgcagg aaccagttct gccgctacga
     4081 ggtggatggc accttttcct acgaggcggc aacatcagac ttcaccgtgc acgagtgggg
     4141 cggggtttcc tatcacgcgg atctgctcgc ctacgggccg ctggccgagg tgctggtgga
     4201 ggggcccatc gaggagattg ccggtccatg gcatgcccag agcgacccca accatcccta
     4261 cgattcccac ctggtgacca ccgacgaccc ggccatgccc tggcttcgtg ccgtttactg
     4321 gggaggcgcg acggagaact atgtctacac ggacaacgca ccgtggggca ccgagcacgc
     4381 ccactttgcc gccgatgcga gcggcaatga cgatctgccg tttcccacgg acgggtcgct
     4441 gtggacgctc aagagcgtgg cgaccggcac cggcggggcg atcatgatct ccagcgcgca
     4501 cctggaggcc tccctgtttc acacggctag cacctttacc gacgggggca tgaccgagtg
     4561 tcagcagtac aacaactaca cgtatctcat ccggcgaatg cagtcccagc tgggcgcgcc
     4621 atttgccgtg cccggctatg acgccttctg cgctccggac agggccggcg aggtgaagga
     4681 gacggccgtc ctgtttccgt ccggcctggc ttaccagaat gcgccgcgaa tcgacacctc
     4741 tggtggaggc ggcggtgatg ggggtggtga tggcggcggt ggcaccggat cgacgtcgat
     4801 ctccttcgac gacggcacct tgggtgactt tcaactggaa ggcgacgggt tgcggccgtg
     4861 gcaggccaat gcgactgctg cctgtaatgg cgcgtacggc gcgatggccg gagtgcccgg
     4921 cggggtggac atggagtcgt tcctgaccct gaccgttcct gcaggcgtga gccgggtaac
     4981 ctaccgatac agctacccga gcgctctgga ccggggcgac gacttccacg tcatcgtcaa
     5041 cggcgcggtc agaaagtcct acgaaacggg cagcggcgcg cagtgcgcga cggattcgtt
     5101 gccggtgacg ccgggggatg agctgcggtt caaatgccgt tccggcggca acggcgagac
     5161 ttgcgccgtg gacgagatcg tcttcgactg aaacctcggc gcccggcgag caaggtcaac
     5221 cctctggcgc tccgcgcgcg gtcacaaccc gtccgacgcc gccgcggcgg ggatcgccgg
     5281 ctgcccggaa gagatccttg gccagctcgg ccacatgcac gccgccgaag aacaggctct
     5341 ggccgggcca ccggcgaatt tcggccgggc ctgcctccag cgcccgaacc gcatcgcggt
     5401 cgaacccgtc ctctatggaa agcagatcgc cctcgagatg cagccgtggt cggcgaaccg
     5461 cggcgtcgac ggagtccccg aagtcgagca ggttgctgat gacctgcacg atggcggtgc
     5521 gaattctgtt cgagcctccc gatcccaggg ccaggcgcct cccggcgacg gtttccacca
     5581 gggtgggcgc catcatcgac gtgagccgtt ggtctggcgg ccagttgccg aacccgtccg
     5641 ggttgatgtc ctcctcgccc agcatgttgt tcagcatcac gccggttcct ggaatcatgg
     5701 tcccgcaccc ctcaccgttg gaaagggtca tggcggcggc gttgcccttt gcgtccacga
     5761 cgctgatgtg cgtggtgccg cggttgaagc gcggcgtccc gagcacctcc cgccggtaac
     5821 gctcgacgag cgccgggtcc agctccgcgt ggctgccggc cgcggcgagc aattccactc
     5881 gggcctgatt ggttgccgcc atgatttcgg ccagggcggc aagatgcgac gggcccccga
     5941 aggtttggtc ggcaacggcc atggcctgga gaagctgcag gccaaagccg atcaggattc
     6001 cgcctgagga gggcggaggg ttggtcagca cggtggcccc ggcgtggcgc aggctcaacg
     6061 gcatgcgttt gacgaccccg tagccgagca gatcctccat ggaaaggtgc ccgccttgct
     6121 cctgcgccag ccgtgccacc gcgtctgcga acgcgccgcg gtagaagcgc cccgcgcctt
     6181 gccgcgcgac ctgctcgagg gtgtcggcca ggtcgctctg gcgcaggagg cgcccctctt
     6241 ggatgccgcc gtagatcgac cgggcctcgc tgctcgctgc gtagatgggc ttgacgatct
     6301 cgaagatgct tgcctgcagg cgctctatgc gcacgccttc ccgtgcggca cgagcggccg
     6361 gttcgaccag ccgagccatg ggcaggctcc ccaggtcgcc gtgaacggct tccatcccgg
     6421 ccagggagcc gggcgttgcc gcggctccca ggccgatgtg gaattcctgg gttgcggtgc
     6481 cgaagtcggc gtggaccgga cgaaagtcca cggccgaagc ctctctaggc gagcgcggcg
     6541 tctggacgaa gaagtcgtag accaccggcg cgcctggagc ggggtgtgcc agcaggaagc
     6601 ccccgccgcc aacggatgcc agcaccggtt cggcgacgca ggcggcgaac tgggcggcga
     6661 tcaccgcgtc gaaggcgttg cctccttcct cgaggatctc cgcggccgcg caggcggttg
     6721 ccgggtgacc gcaggcgacg gcccccaggc caccccgcgc cgggacgccc gcccctccgg
     6781 cttgagtcgt caaagccagg cagacctagg ccgggtcggg ggcccgcgag cggggccaca
     6841 cctccggcag gtcggtcgcc agccagcgta ccagcgccgc gttgacgtcc agcggacgct
     6901 cctgggccat ccagtgcccg gagtagacgg tggtctcggt aagccgccgg cagcgctcgc
     6961 gcatgggatc ggcaagcgtg gagtcgtagc attcgcagac gtagtcgtag cgggcgccga
     7021 gaaacagaac cggcatgtcg aggtagccat cgttgaccgc ttctgccgcg taggcggagt
     7081 tttcggcgtg gttcatgtag taggcgttcg ggccgaagaa gccgttgcgg gcgagggcgt
     7141 cggcgtagac cgccaggtct tcttcgctga tcacgtcatc gtccctgggc acgtcgggcg
     7201 cttctccggc aggaccgaac cagccgccgt tgattcgggt catcgccgtc gccgccggcc
     7261 ggccggcgcc gttggggtcg cccctgcgaa acaggagctt tacggtgttc agggggttgg
     7321 catccatttc ggccctggcc ctgtcgaagt gctcctgata gaacaactgg tattcccatt
     7381 gccccgccgg gtactcgggc ccgtagacgt cccggttcac ttggtccacg cacgcctgta
     7441 atcccctctc cagggtgaag tagggcacgc agagcccggc cacggcggtg caggcctcgg
     7501 gatgatggct cgctatgctc cacacagtcg gcgcccccca gtcgtgacct acccagatcg
     7561 cttccgtgcg tcccaagccc tccagcagcg ccatcatgtc gcccaccacc gcgcgctgag
     7621 cgtagtcctc gtatcgctcg taggtcgagg atcttccgta ccctctcatg tcgggggcga
     7681 cggctcgata accgagcgcg gccagcgcgg gtagctgatg ccgccagctc agggagagct
     7741 cgggccagcc atgaacgaat atgatcagcg ggccgtcctc ggggcccgcg gcgagataga
     7801 atgtcgtgtg gccctcgcgt gttgcggtgt gctcctccag tgccagtgcc aaagcccttc
     7861 tcccgtgtct tgacctcatc gtgattgttc gaatcatatc ccttatggca aacggggcac
     7921 ctaccaggat ttcgccgacg ggcgcgggcg cctctgcggg caccctgatc tgggaactgc
     7981 ccatgaggat ctggcactgg gtattggcag cctgcgtaac gggctctctc tacaccgggc
     8041 ttgcgggtga gattgctctc atggaacgcc accagcagtt cggctacggc gttctcggcc
     8101 tgctgctctt tcggctgggc tggggcttgt ggggaggtcg atatgcgcgt ttcggcaact
     8161 accgcctggc ccctcgacgc ctgattgagc actttcgcgg cctcgaccac ggcgctgcgc
     8221 acaccgcgcc cgggatggcc ttcaccatcg tgttcctgct cgcgctggcc gcgcaggtcg
     8281 gcacgggttt gtttgccact gacgacatct tcacccaagg cccgttgagc cgcctcgtcg
     8341 agaacgatac ggcccgcgcg ctcacgggca tccaccaccg ggtgtactgg ctgatcctcg
     8401 cggccctggc cgcccacctg acagctcacg ccgtttacgc gctgcggcgt gacccgatgc
     8461 cgctggccat gatcaccggt cgcaaacgcc tcgacctgga gccggcgcgt gcggcctgga
     8521 cggcggcgtt gctcaccgca ttgggagccg ctgctctggt ctgggcgatg ctggagctcg
     8581 cctgagcgtc agtcggcgcg gtagttgtcg tggcagcctt tgcaggactg acccagggcc
     8641 tgaaaggcct ggccgagcgc cgcatcgtcg ttggatcgtg ctgcagcgct gaaggccgtg
     8701 gccgcttcgc gcatggcttc cacccgcccg gcaaaatcct cgggttcttc ccagatcgcc
     8761 ggcagggcgt cgccgccctc ggatccttcg gggaacagcg tgccgacgat gccggacagc
     8821 gcagccagcg catcggcgtg gatggtcatg tgggaggcgt gcggcacctt ctgcctcaag
     8881 atgtccacgg ctgcctgcat gtgcccgccg atggcctcca tggtgtgctc gcggtattcg
     8941 agctcccctt cgcttgcggt ggccgcgctg gcggcaccca gcgccactaa cccgatcagc
     9001 cagtctcgac ctcgcatgaa ctctcctcat cgttgctgga gttgattgcg cgcaccgtat
     9061 ttgaaatccg gctccgcggc aattgccgcc gtccgggagc ggtgcagatc cggtcccttc
     9121 tttggcagtg ttcagcgggc cggcccggtg atagtttacg ccaccatgcc taatgccccg
     9181 tcacctctgc cggaagcgca tccagcggtg cagcccgtgc ccaacggcat ggaccgcttt
     9241 gccatcacgc tgtccgggct ctgcctcctc cactgcctgt tgattccggt ggtgctggcc
     9301 atggcgcctg cattttccag cctcgtgctc ggcacggaga cgaccgtgca ctgggttttt
     9361 ctggggctgg ccgtgccgac cagcgtctgg gccctctggc gcggctacgg aagatgccag
     9421 cacctgcgag ccctggtgct gggcatcgcc ggcctgctcg tgatgttcct gggcgtttct
     9481 caccttttcg atccgcggct cgaggttccg ctcacccttg tcggcgtcac catggtggtg
     9541 gtggctcacg tgctcaacct gcgaattccc gagccggctc cgcgcgggtg acttggtttc
     9601 gccgaatcga ctatgatcgg tcggttcgga tcgagcatag ggaggagaac gctcatgtcc
     9661 acgtttcatg acattcgaat ggaaagcatc gacggcgagc ccgtggagct gtccaagttt
     9721 cagaacaatc tctgcctgat agtcaacgtc gcgagccgct gaggcctcac ccctcagtac
     9781 gcaggtctgc gtacccttca ggaacaatac ggcgaccggg gttttaccgt gctcgccttc
     9841 ccgtgtaacc agttcggtgc ccaggagcca ggctccaacg aggagatcct ggattttgcg
     9901 agctcgaagt tcgacgtgaa cttcccgatg ttcggcaaga tcgaggtcaa cggctcgggc
     9961 gcgtgcgagc tgtaccaata tctcaaagcc gcccgtgcag acgaggaagg caatgcggac
    10021 atcgcctgga acttcacgaa gtttctcgtg gacggggacg ggaacgtcat cgcccgtttc
    10081 gcgccgcccg tgacgccgga ggagatcggc gagcagatcg ggtcgctgct gccgggctag
    10141 catgctcggg ccccggaccg tgcggggccc agatttctca gggccgctcc acgcgcagca
    10201 cccgcccttc gtcggtgacc aggtagaggt gaccctccgg gcccacccgg atgtcccgca
    10261 gccgctcgcc cagttccccg aacagggcct cctcctccac gacgcgatcg tcgcgcaggc
    10321 gcactcgccg cgcttccctg tccaccagcg cgccgacgaa caggtcgccc tgccactcgg
    10381 gaaagacgtc gccccggtag acggcgaggc ctgacggcgc gatggaaggt acccaggagt
    10441 gcagcggctg ctccatgccg ggcatctgct ggtaaggaga gatgcgcgcg ccgctgtagt
    10501 cgaggccgaa cgtgatgacc ggccagccgt agttccggcc gggcctgagg agattgatct
    10561 cgtccccgcc gcgaggaccg tgctcgtgga gaaatacccg gccggtcacg tgatccacgg
    10621 ccaggccctg cgggttgcgg tgcccccagg tccacaccgc cgggtccgcg ccctcgcgtc
    10681 tcacgtaggg attgccatcg ggaatgcttc cgtcatccct gatgcgaacg gttttgccga
    10741 gcagcccgga aagatcctgg gcttcctccc ggtggtcgaa gccgtccccg gtggtgaaaa
    10801 gcaacgtccc gtcgggaagg aacaggaact tgccgccgta gtgctggggc gtcgtcttgt
    10861 tcgggcgggc cgtaaagatc acctctaagt ccttcagcga gttgccatcc aggcggccgc
    10921 gggcgatgcg cgtggtgttg gctcgcggcg tgccgtgggc ataggagatg tagatcagcc
    10981 ggttttcagc gaagtccggg tggggggcca catcgaacag gccgccctgg gcgcggaaca
    11041 acacctcggg gattccttcc atgggcgagc cgatctcgcc ctcgggcgag atctgccgaa
    11101 ggcgaccggg acgctccgtg accagcaacc gcccgtcggg aaggaaggcc acgcaccacg
    11161 gcagctccag gccctcggcg acctcgacca ggcggtagtc ggccgacatt gcctcggtgg
    11221 agatggccag agacagcgtc agaaagatta gcgttgaagg ctttatggcg acttccggat
    11281 tgaatgaggg ttcgaagggt gaagcgaagc cgctatgcta ggacatctgc tcagagaggt
    11341 gaaaccattg gccgcgaccg tgatcgccag aatcgcaggc gcttatctcc tatccgtgct
    11401 ggtcgcctac ctcctggccg ccacggccgc tacccaggcc tccctggctg gcctggaagg
    11461 actgggcgtg accatcacgg tgggtgagcg gatatccacc acgggccagg acctgatggg
    11521 gatgttgccc acttacgggc tgctgattgc catcggtttt gccatcgcgc ttcccattgc
    11581 cgccgccttc gcccggttca ttccttcctg gcgggccgtc gggctcgtcg cggcaggagc
    11641 ggccgcggtc ggcctcattc atgtcgggct gaactcggtg ttcgagaccc acctcgttgc
    11701 cgcgacccgg acgacggtgg gccttctggt tcaggtcatg gcgggtggcg tcggcggcta
    11761 cgctttctat cggctgcggg acccgtgact gaggcaggca aagtcggctc gtgcagccga
    11821 cgactcctga gaagagcaga aggaccaggg gagcgcggga ttcatgaggg agccgcacag
    11881 ctcagccagc ggtcgcgggc cggccgccat gggtcggttc ctggatgcgg tcgagcgcgc
    11941 cggcaaccgt ctacccgacc cggcgctcct gttcttcttg ctcctgctgg cggtctgggc
    12001 gctatcggcg ctgctctcgc agctcgacta cggtcacgtc gaccctcgca gcggggatcc
    12061 ggtggaggtt cgcaacctgc tgaccggcga ggccctgacc agcttcttcg ccggcatggt
    12121 cgagacgttc gtggggtttc acccgctggg cgtcgttctc ctcgccatgc tcggtatagg
    12181 ggtggccgac cacaccggct tcatcaaggc ggggctgcgc cggctgctca gcctgacttc
    12241 gcagaggctg ctcaccccga tgctctttct gatcggcatt ctcagccaca gcgccgtgga
    12301 tgccggatac gtgctcgtca tacccctggg cggcgtcatc tttcaggcgg ctggaagaca
    12361 ccctctggcg ggaatcgcgg tggccttcgc cggcgtctcc ggagggttca gcgcgaactt
    12421 tctgccctcc gccttagatc ccatgctgca gggcatcagc caggccggcg cgcagctgat
    12481 ggatccgggc ctggtgctga acccgctcaa caactggttc ttcacctccg cctccacgct
    12541 gctcgtcgtg cttctcggat ggtacctgac ggctcggatc gtggagccgc ggctggctga
    12601 cgagcccctg gacggcgatc tggccgatgc gcccgccctg gatgatcttt ccgacgaaga
    12661 gcgtcgcggg ctacgcgcgg ggcttgtcgc catgctgctc ggaattgcgc tgctggcggt
    12721 cacggcctgg ccggacagtt cggcatggcg ctcgggcggc cagctgaccg ccgcgggtgc
    12781 gcccctcatg cagagcatcg ttccgctcat ctttctgctc ttcatcattc ccggcgtggt
    12841 ctacggttac gtctccggca cggttcgggg gcatcgggac gtgatcgcgg gcatgagcca
    12901 ggccatgagc gggatgggtt actacatcgt gatggccttc ttcgcttcgc tgttcatcgc
    12961 cgagttcgga cggtccaacc tgggcgtgct cgtcgcgctg cagggagcgg cctggctcga
    13021 ggcggccgcc atgccggcgg cagtcaccgt ggtgggggtg gtggccatca ccggtgtggt
    13081 gaacctcttc gtcggctcgg cctcagcgaa gtgggcgcta ctcgcgccag tgctggttcc
    13141 gatgctgatg gctctgggca tctcgccgga tctcgcccag gccgcctacc gagtaggcga
    13201 ctcctcgacg aacatcatca cgccgctgat gccctacttt cccctggtgg tggtcttcgc
    13261 gcagcgctac gtgacgcgca cgggcatcgg caccatcgtg gcgatgatgc tgccttactc
    13321 agtgggcttc ctcgtggtgt ggacgctttt cctgctggtt tactggatgc ttggaattcc
    13381 gctgggcttg caggcgagct acggctatcc cgggggttga gcccgatcgt cgccgtaggg
    13441 gacccgccgg ccgtgatcgg ctagagtagg tggctgatca gaatcattgg aatcggggtc
    13501 ttctcatgag cttatttcgc atcgcgttgc gggtaatcgt gttgctggcc ttgccggttt
    13561 gggccgtggc gggaaccctg gacgagatag ccgcctcggg cgagttccgg cttgcctacc
    13621 gagcggacac gccgccgttt tcgtcgcgcg gcaagggcgg cgagcccgag ggcttcacca
    13681 tcgacctgtg ccgcatcatt gcccgtgacg tccaggcagc gctgaacctc gacgagatga
    13741 aaatcacctg ggtgcctgtg acggcggaga accgattcga tgccatcgtc tccggccggg
    13801 cgcatctgga gtgcggctcc tccacggtca ccctgtcccg gcaggagcgc gtcgacttct
    13861 ccaacctgac ctatgtgacc ggcgccagcc tcatgcgctg ggcggactcg gacatagatc
    13921 gggtcggtga tctggacggt cggcgcgttt cagccatcgc gggcacgacc acggagaagg
    13981 tcctcaagga aaccctcgag cgagatggga ttgatgccaa ggtggtggtc gtgggtgatc
    14041 acgcggaagc gctgcagctg ctgctggaca gaaagatcga tgccatggct ggcgatcagg
    14101 ccacgctctt cggtatcgga ttcaagaccc agggtgatga cgacctggtg atcaccgagg
    14161 atcttctctc ctttgagcca tacgctctgc cgctcaggcg caacgacgcc gactttcgcc
    14221 tggcggtcaa ccgctcgctc tccgagctct acgttcgagg tgacgtgggt cggtcctggg
    14281 agaagtggtt cggctcccac ggggtgcgcc cgacgcggct actgctgatg ctctaccgcc
    14341 tcaactcctt ctcggagtag ggcgcgcgac ggctcgtcag gcggccgccc gctgggctgc
    14401 tcagtgcgcc ccgtcgccca cgggaaccag cgctatgggc tccatgtcgc gcaggtaggc
    14461 atcgtccacg gcgtccaggt tgttcgcctt catgatcgag tgcagggtac ggacgtagtc
    14521 cgctccccgt tccgagtatg aggtcagggt gcgggccagc tcccagccgc tgggaagctc
    14581 acccatggca cgcagttccg ctcggcgtgc gcgcaggcgc cggtaggccc ggtgcgtgtt
    14641 cagattccgc atgtacgcgc gtatggaatc cagcggtgtc tcgaagcgag cgacgtagta
    14701 ctcgcccaac tcctcacggc gttccagagg aatgatgccg tcgtcccatg cccattggcc
    14761 gaacagggcg ttgcccacgt cggcgaagcg cgacgtgccc cagccgctct cctctgcggc
    14821 ctgagccagg atcagagatg ggggcagaac gtccacgcgc agcatcagct cctcgatggc
    14881 ctccggcccg gcggcacttt cgtccctcag ccggtaacgg gcggccagat tcgccagcca
    14941 gtcggcatca tccggtgcca gctgcccggg gcccttggcc aactcgagca gccgagctcg
    15001 atcagcggcg accag
//
LOCUS       DBNHQR010000492         3802 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_490985, whole genome shotgun sequence.
ACCESSION   DBNHQR010000492 DBNHQR010000000
VERSION     DBNHQR010000492.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_490985"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /gene="ftsY"
                     /locus_tag="AC16VR_11150"
     CDS             complement(<1..905)
                     /gene="ftsY"
                     /locus_tag="AC16VR_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIR6011758.1"
                     /translation="MSEQDQSSAEGGGLWSRLKRGLGQTRNQLAEGVGNLLLGEKEID
                     DEVLSELETALLLTDVGIDTTREIMDKLAVRVRRKELNDTVALHQALGSLLKELLLPV
                     EKPFTIAQDKRPFVIFMVGVNGAGKTTTIGKLAKRLRDDGLNVVLAAGDTFRAAAVEQ
                     LQTWGERIGVPVIAQGQGSDSASVVYDAVAAARARGHDVVLADTAGRLQAKANLMEEL
                     AKVKRVVNRMDPDAPHEVLLVLDAGVGQNALSQVREFDAAVGVTGLIFTKLDGTAKAG
                     VLFGIARQVMKPVYFVGVGEGADDL"
     gene            946..1533
                     /gene="rsmD"
                     /locus_tag="AC16VR_11155"
     CDS             946..1533
                     /gene="rsmD"
                     /locus_tag="AC16VR_11155"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HIR6011759.1"
                     /translation="MSDWHGSLTILRMASNQVRIIGGKWRGRKLAFPDAATLRPTLGR
                     VRETLFNWLAGRIAGACCLDLYAGTGALGFEALSRGAAEADMVERARTAVAALRRNAQ
                     LLKAPARVHGIPAERFLQATDRTWDVIFLDPPFADDALPRALEVIAERGLLRPGGCVY
                     FEQAERGGEEPAGPGWRLLKKSRAGASEFGLLVRD"
     gene            1576..2049
                     /gene="coaD"
                     /locus_tag="AC16VR_11160"
     CDS             1576..2049
                     /gene="coaD"
                     /locus_tag="AC16VR_11160"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HIR6011760.1"
                     /translation="MRTVVYPGSFNPITNGHTDLVKRALNLFDHVIVAIGTSAQKDPS
                     VALKNRIDLCRLVLAEFGDKVTVEGFNSLLVNYVRERNARFILRGLRTVTDFDYEFQM
                     AEMNQSLAPEIEYVFLPTAQNCSFISSTLVREIASLGGDISQFVHPAVVEAYHRD"
     gene            complement(2098..2409)
                     /locus_tag="AC16VR_11165"
     CDS             complement(2098..2409)
                     /locus_tag="AC16VR_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011761.1"
                     /translation="MKRMKRAILAFTLALFMATPALAAPEDSPSAFTMTGDLVVARPL
                     GVAMTAIGAAVFVVSLPFSALGGNVGESADALVMGPVNETFVRCLGCPSAGRRQAPPA
                     N"
     gene            complement(2472..3344)
                     /gene="mutM"
                     /locus_tag="AC16VR_11170"
     CDS             complement(2472..3344)
                     /gene="mutM"
                     /locus_tag="AC16VR_11170"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010489454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HIR6011762.1"
                     /translation="MPELPEVETTRRGVAPLVDGRYLLDWNLRNPNLRWPVQLPDELR
                     GSRIARVRRRAKYLIFELEPSALGAAGSLIIHLGMSGNLRVLPQGSPYLPHDHVELAL
                     ASEGEGAATRVLRLNDPRRFGSLHWQPGEAEAHWLLAGLGPEPLSEAFSGEYLKRAAR
                     GRRVAVKNFVMDGRVVVGVGNIYANEALFLAGLRPTARAGRVTRLGYDRLVAAIKQVL
                     TEAIDMGGTTLRDFVNQDGNPGYFKQSLNVYGRLGLPCHRCATPLKGTRTGQRATVFC
                     PNCQGAQSFSGRNR"
     gene            complement(3373..3528)
                     /gene="rpmG"
                     /locus_tag="AC16VR_11175"
     CDS             complement(3373..3528)
                     /gene="rpmG"
                     /locus_tag="AC16VR_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIR6011763.1"
                     /translation="MRDKIKLVSSAGTGHYYTTDKNKRTTPDKLEMKKYDPVVRKHVV
                     YTEAKIK"
     gene            complement(3554..3790)
                     /gene="rpmB"
                     /locus_tag="AC16VR_11180"
     CDS             complement(3554..3790)
                     /gene="rpmB"
                     /locus_tag="AC16VR_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011064219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HIR6011764.1"
                     /translation="MSRVCQVTGKRPITGNNVSHAHNKTRRRFSPNLHYHRFWVDSEK
                     RFVRLKVSSQGMRIIDKLGIDQVLADMRKRGEKV"
BASE COUNT          643 a         1270 c         1185 g          704 t
ORIGIN      
        1 ctgaggtcgt ccgcaccctc gcccacgccg acgaagtaga cgggcttcat gacctgacgc
       61 gcgatcccga acagcacccc ggccttggcc gttccgtcca gcttcgtgaa gatcagcccc
      121 gtcaccccaa cggccgcgtc gaactcgcgg acctggctga gggcgttctg ccccacgccg
      181 gcatcgagca cgagcaggac ctcgtgggga gcgtccgggt ccatgcgatt cacgacccgc
      241 ttcaccttgg cgagttcctc catgagattg gccttcgcct gcaggcgccc cgcagtgtcc
      301 gccagcacga cgtcgtggcc gcgagcccgg gccgccgcca cggcgtcata gaccaccgag
      361 gcgctgtcgg acccctgacc ctgggcgatg accggcacgc caatccgctc gccccaggtc
      421 tgaagctgct ccacggccgc agcgcgaaag gtgtcgcccg cggccagcac cacgttcagg
      481 ccgtcgtcgc gaagtcgttt ggccagcttg ccgatggtcg tcgtctttcc cgcgccgttg
      541 acgcccacca tgaaaatcac aaacggacgt ttgtcctgtg caatggtgaa gggcttctcg
      601 acgggcagta aaagctcctt gagcagactc cccaatgcct ggtgaagggc gacggtgtcg
      661 ttgagctcct tgcggcggac gcgcacggcg agcttgtcca tgatctcccg ggtggtgtcg
      721 atgccgacgt cggtgagcag caacgcggtc tccagctccg agagaacttc gtcgtcgatc
      781 tccttctcgc cgagaagcag attgccgacg ccttcggcca gctggttgcg cgtctggccg
      841 agcccccgct tcagccggct ccacagccct cctccctccg cactcgactg atcctgctcg
      901 ctcacgatga tccttttccc ggcgatgcgc tcgccggtct acggcttgtc tgactggcac
      961 ggttcgctca ccatccttcg gatggccagc aaccaggtgc gcattatcgg cggtaagtgg
     1021 aggggacgaa agctcgcctt cccggatgcc gccacgctca ggcccaccct gggtcgcgtc
     1081 cgcgaaactc tgttcaactg gcttgcgggc cggatcgccg gcgcctgctg cctcgatctc
     1141 tacgccggca ccggcgcgct gggtttcgaa gcgctgtccc gcggcgccgc ggaggcagac
     1201 atggtggaga gggcgcgtac cgccgtggcc gcgctgcgac gcaacgcgca gctgctgaag
     1261 gcgccagccc gcgttcacgg cattccagcc gaacgtttcc tgcaagccac ggaccgcacc
     1321 tgggacgtga tcttcttgga tccgccattc gccgacgatg cgctgccccg cgccctagag
     1381 gtgatcgccg agcgcggcct gcttcggccc ggcggctgcg tttacttcga gcaggccgaa
     1441 agggggggcg aggagcccgc cggccctggc tggcgcctgc tgaagaagag ccgcgccggc
     1501 gccagcgagt tcggcctgct tgtcagggat tgaccgcccc gataccatcg cgggcttctc
     1561 agtggggtgg ggacaatgcg cacggtcgta tacccgggtt cgttcaaccc gatcactaac
     1621 ggacacacgg atctggtcaa gcgggcgctc aacctcttcg accatgtcat cgtggccatc
     1681 ggcacgtcgg cacagaaaga tccttcagtg gcgctgaaga accggatcga tctctgccgc
     1741 ttggtgctgg cggagttcgg cgacaaagtc accgtggaag gattcaattc cctgctggtg
     1801 aactacgtgc gggaacgcaa cgcgcggttc attctgcgcg gcctgcgcac ggtgaccgac
     1861 ttcgactacg agtttcagat ggcggagatg aaccagtcgc tagcaccgga gatcgagtac
     1921 gtgtttctgc ccacagcgca gaactgctct ttcatttcgt ccacgctggt gcgcgagatc
     1981 gcctccctgg gcggagacat ctcccaattc gtccatcccg cggtggtcga ggcctaccac
     2041 cgggactagc caccagcacc ggggccccta tcgaagagcc ccggcatcag ggggaaatca
     2101 gttggcgggc ggcgcctggc ggcgaccggc gctggggcaa ccgaggcaac gcacaaacgt
     2161 ttcgttgacg gggcccatga ccagcgcgtc cgcggactcg ccgacgtttc cgccaagtgc
     2221 cgagaacggc aagctgacga cgaatactgc ggcgccgatt gccgtcatgg cgacgccaag
     2281 gggcctggcg acgacaagat cgcccgtcat cgtgaatgca gagggtgagt cctccggcgc
     2341 agcaagtgcc ggagtggcca tgaacagggc cagggtgaat gccagaattg cgcgtttcat
     2401 tcgcttcatg tcctttttgc tagtcaaccg ccctcgaccc ttttcctggt gccgagcgca
     2461 ccgcgcctac tttagcgatt gcgtccgcta aaactttggg cgccttggca atttggacaa
     2521 aaaacggtag ctcgctgccc ggtgcgggtc cctttgagcg gcgtcgcgca gcgatggcag
     2581 ggcaatccga gccggccata gacgttgagc gactgcttga aataacccgg attgccatcc
     2641 tggttgacaa aatctctcaa tgtggtgccg cccatgtcga tggcttccgt cagcacctgc
     2701 ttgatggccg ccaccagccg atcgtagcca agcctggtga cacggcctgc ccgtgccgta
     2761 ggccgcaggc cggccaggaa cagtgcctcg ttggcataga tgttcccgac gcccaccacc
     2821 accctgccgt ccatgacaaa gttcttgacg gcaacccggc ggcctctggc cgcccgcttc
     2881 agatactctc ccgagaaggc ctcagagagc ggctccgggc cgagccccgc caggagccag
     2941 tgggcttcgg cctcacccgg ctgccagtgc aggctgccga accgacgcgg gtcattcagc
     3001 cgaagcacgc gcgtcgcggc cccctcgccc tccgatgcga gggccaactc cacgtgatcg
     3061 tggggcaggt agggcgagcc ctggggcaga acccgcaggt ttccagacat gcccaggtgg
     3121 atgatgagac tgcccgcagc gcccagggcc gatggctcga gctcgaatat gaggtacttg
     3181 gcccggcgcc tgactctggc tatccgggaa ccgcgcagct catcgggtag ctgcaccggc
     3241 cagcgcagat tggggttgcg cagattccag tcgagcaggt agcgcccgtc gaccaggggt
     3301 gctacgccgc ggcgggtggt ctcgacttca ggtagttcgg gcaccgatca gcagggggcg
     3361 ctgatccgcc ggttacttga tcttcgcttc ggtatagacg acgtgcttgc ggacgacggg
     3421 atcgtacttc ttcatctcca gcttgtccgg cgtcgtacgc ttgttcttat cggtggtgta
     3481 gtagtgtccg gtgcccgcac tggaaaccag ctttatcttg tcgcgcatct gtctttacct
     3541 gccgttagcc cactcagacc ttctcgccgc gcttgcgcat gtcggcaagg acctgatcga
     3601 tgcccagctt atcgattatg cgcatgccct gggacgaaac cttcagccgc acgaagcgct
     3661 tctcgctgtc cacccagaag cgatggtaat gcaggttagg cgaaaaacgc cggcgcgtct
     3721 tgttgtgcgc atgactgacg ttgttgccgg tgatcggccg cttacccgtt acctgacaaa
     3781 cgcgcgacat ggtcccgttt tc
//
LOCUS       DBNHQR010000493         3623 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1631464, whole genome shotgun sequence.
ACCESSION   DBNHQR010000493 DBNHQR010000000
VERSION     DBNHQR010000493.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1631464"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="AC16VR_11185"
     CDS             complement(<1..947)
                     /locus_tag="AC16VR_11185"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6011765.1"
                     /translation="MSTSLWIALRYLVTRRRQFAAFVTWVSVAGLALGVLVLTVVVSV
                     MNGFDAELRHRILGSVPHLIIEGESVTDPNVGSLLDRPEIVGSFDFFLGSGMVTRSGA
                     VHPVTVYGLDAEGLTGLGEIGQAMRSGRLSGLFEIPRGIVIGEPLAAHLGLLPGDSVA
                     VVISEPTASGVQPRFGRYELVGTFEVGAVIDYSVVVVARDALDGLRLDEVGVNGARLV
                     LADPLDAPRLAAELAMARPAWNLVSWTDSYGELFQAVGLEKMVMFIILLMVVAVAAFS
                     IVSGQMMAVADKRADIAILSTMGASRRTILGIFLLQGVLV"
     gene            complement(944..1618)
                     /locus_tag="AC16VR_11190"
     CDS             complement(944..1618)
                     /locus_tag="AC16VR_11190"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008169187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6011766.1"
                     /translation="MSEPVLEARGLVKSFTQGTTRVVVLSGADLDVVAGERVAIVGRS
                     GSGKSTLLHLLAGLDDADAGSVIVGGKDMTAGSPAQRAHIRNAHMGFVYQFHHLLPEF
                     SALENVIMPLRLGGMDPAAAAERARGMLERVGLAERFEHRPSALSGGERQRVAVARAL
                     VGSPLVVLADEPTGNLDRDNAEHVFRMMCELSDEHGVGFVIVTHDEEMLGSMHRVLRL
                     ENGRLA"
     gene            complement(1611..2810)
                     /locus_tag="AC16VR_11195"
     CDS             complement(1611..2810)
                     /locus_tag="AC16VR_11195"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6011767.1"
                     /translation="MGSRYAFSRRSTLSFISLVAISGLALSVAVLVIVTSVVNGFDRE
                     LRERVFGVLPHLSFVGREPLAFSLDDLEALRAIPGVRGIAPFVEGAALVAVADGVGAV
                     MVTGMEPESYESVSDVHQFVRGRMPQAGEYGVLLGDQVAQRLGVAIGDAVTLVLPSAT
                     VTPAGLFPRQKRFVITGLLDSQSDLDGRAAYVRLTVAQKLFRLGDRIHGYQARLDDLF
                     AALEVGSAGVLALGPQRLFARSWMRTHGNLYQAIGMLKSTLFVLLSFLIAVAAFNLIS
                     TLVMVVDQRSGDIAVLRTLGSDTSTLIWTFVILGVLLGGAGIAAGLGLGAALALALPG
                     LYEWLTTLLGADLLAQYFVTYLPVELQPGDLLGIAVTAFLLCVLSTLYPARRAAGLQP
                     FKVLAYE"
     gene            complement(2915..3139)
                     /gene="nqrM"
                     /locus_tag="AC16VR_11200"
     CDS             complement(2915..3139)
                     /gene="nqrM"
                     /locus_tag="AC16VR_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003091178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HIR6011768.1"
                     /translation="MTILLTFLCMMAVVGLMAIGVMFGRRPIAGSCGGLKNLGIGGEC
                     EICGGDPARCESPGDTRLAVDATNRARRSR"
     gene            complement(3141..>3623)
                     /locus_tag="AC16VR_11205"
     CDS             complement(3141..>3623)
                     /locus_tag="AC16VR_11205"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF014477.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HIR6011769.1"
                     /translation="DRIAQRLFELECSDFLVDVGGEVRASGVNPQGAAWRIGVEVPDP
                     DSFGAIQRVLRISGSGLATSGDYRNFLEVDGERFAHTIDPRTGYPVDHTLASVTVVHE
                     SAMLADGYATLLNVLGPQEGFSLAEGLGLPVLFLIRTPNGFEERYTVGLEQFLGQTDY
                     "
BASE COUNT          767 a         1268 c         1089 g          499 t
ORIGIN      
        1 accaaaactc cctgcagcag gaagatgccg aggatcgtcc ttcgcgaagc gcccatcgtg
       61 ctcagaatcg caatgtccgc gcgcttgtcg gccaccgcca tcatctgccc cgatacgatg
      121 ctgaacgcgg ccaccgcaac caccatgagc agaatgatga acatcaccat cttctccagg
      181 cccaccgcct gaaagagctc gccgtagctg tccgtccagc tgaccagatt ccatgcaggc
      241 cgagccatcg ccagctccgc tgcgagccgc ggcgcatcga ggggatccgc cagaaccagg
      301 cgcgccccgt tgacgcccac ctcgtccaaa cgcaagccgt caagcgcgtc gcgggcgaca
      361 acgaccaccg agtagtcgat gacggcgccg acctcgaaag taccgaccag ctcgtagcga
      421 ccaaagcgtg gctgcacgcc ggacgccgtc ggctcggaga tcaccacagc caccgaatcg
      481 cctggcagca ggcccaggtg cgccgccagc ggctcaccaa tcacgattcc ccgcgggatc
      541 tcgaacaggc cggacaaacg tccgctgcgc atggcttgac cgatctctcc aagcccggtg
      601 agtccttctg cgtccaggcc gtagacggtc accggatgaa ccgcgccgct tcgggtgacc
      661 attcccgaac ccaagaagaa gtcgaaagag ccgacgattt ccggccggtc gagcagcgaa
      721 ccgacgttcg ggtcggtgac gctctcccct tcgatgatga gatggggcac ggatccgaga
      781 atgcggtgcc tcaactcggc atcgaacccg ttcatcacgc tgacgaccac cgtcagcacc
      841 agaacgccca gcgccagacc ggcaacggag acccacgtga cgaaagcggc aaactggcgg
      901 cgccgcgtga ccagatagcg cagagcaatc cagagcgacg tgctcacgcc agccggccgt
      961 tttccagacg caggactcgg tgcatgctgc ccagcatctc ttcgtcgtgg gtgacgatga
     1021 caaaacccac accatgctca tcgctgagct cgcacatcat gcggaaaacg tgctccgcgt
     1081 tgtcccggtc gaggttcccc gtcggctcgt cggcgagcac caccagcgga ctgcccacca
     1141 gcgcgcgggc aacggccacc cgctggcgct ctccgcccga cagagctgat ggccgatgct
     1201 cgaaccgctc ggccagaccc actcgctcca gcataccgcg cgctctctct gcggcggcgg
     1261 cagggtccat gcctccgagc cgcagaggca tgatgacgtt ttccagagcc gagaactccg
     1321 gcagcagatg gtgaaactgg tagacgaagc ccatgtgcgc gttgcggatg tgcgcccgct
     1381 gcgcgggaga tcccgccgtc atgtccttgc cgccgacgat caccgacccc gcgtcggcat
     1441 cgtcgagacc tgccagcaga tgcaacaggg tgctcttgcc cgagccggac cggccgacga
     1501 tggcgacccg ctcgccggcg acaacgtcga gatccgcccc cgacaacacc acgacccggg
     1561 tcgtgccctg agtgaacgac ttgacgaggc cgcgggcttc gaggaccggc tcactcatac
     1621 gcaagaacct tgaaaggctg taaccccgcc gcgcgccgcg ccgggtacag ggtgctcaaa
     1681 acgcacagca aaaaggccgt cacggcgatg ccgagaagat cgcccggctg cagctcgacc
     1741 ggcaggtagg taacgaagta ctgggccagc agatcggctc ccagaagcgt cgtcagccac
     1801 tcatacaggc cgggaagagc cagagccagc gcggcgccca ggccgagacc cgcggcgatc
     1861 ccggcaccgc cgagcagaac gccgagaatg acgaacgtcc agatcagcgt cgaggtgtca
     1921 ctgcccagcg tccgcagcac ggcaatgtcg ccgcttcgct ggtccaccac catcaccagg
     1981 gtcgagatca ggttgaacgc ggccaccgcg atcagaaacg aaagcaggac gaacagcgtg
     2041 ctcttcaaca tgccgatggc ctgatagagg ttgccatggg tacgcatcca ggaacgcgcg
     2101 aacaggcgct gcggaccgag ggcgagaaca cctgccgaac ccacctccaa cgcagcgaac
     2161 agatcgtcca gcctggcttg atacccgtgt atgcgatcgc caagccggaa cagcttctgc
     2221 gccacggtca ggcgcacata ggccgcgcga ccgtccaggt cggactgcga gtcgagcagc
     2281 cccgtaatca caaagcgttt ctgccgcggg aacagccccg caggcgtgac cgtcgccgac
     2341 ggcagcacca gggtgacagc atcgccaatg gccacgccga gccgctgggc cacctgatca
     2401 ccgagcaata ccccgtactc gccggcttga ggcatgcggc cgcgaacaaa ctgatgcacg
     2461 tcggagacgc tctcgtagct ttccggctcc atgcccgtga ccatcacggc gcccaccccg
     2521 tcggccaccg cgaccagcgc ggcgccttca acgaatgggg cgatcccccg gacgccggga
     2581 atggcccgaa gcgcctccag atcatcgagg ctgaatgcca acggctcgcg cccgacgaag
     2641 ctcagatgag gaagcacgcc gaaaacccgc tcgcggagtt ctctgtcgaa gccgttcacg
     2701 accgacgtca cgatcaccag cacggcaacg ctgagggcga gcccgctgat ggcgaccagg
     2761 ctgatgaagg aaagcgtgct ccgtctcgag aaggcgtagc gactgcccac gctgagcgca
     2821 actgctttga tcaatgcaaa ccgacccggc ccgggggagg ccgcatgcta acacgtcggt
     2881 agcgccgcgc gccctcggga agaagccctg gtaatcagcg ggagcgtcgt gcccggttgg
     2941 ttgcgtccac ggcaagacgg gtgtccccag gcgattcgca gcgcgccggg tcgccgccgc
     3001 agatctcgca ctcgccgccg atccccaggt tcttcagccc accacagctg cccgcgatgg
     3061 gccggcgccc gaacatgacg ccgatggcca tgagccccac caccgccatc atgcagagaa
     3121 aggtcagaag aatcgtcacg tcaataatcc gtttgcccga gaaactgctc caagccgaca
     3181 gtatacctct cttcgaagcc gttcggagtt cggatcagga aaagtaccgg taatcccagc
     3241 ccctcggcca gggaaaagcc ctcttgcggc cccaggacgt tgagaagcgt ggcgtaaccg
     3301 tccgccagca tggcggattc atgaaccacg gtcaccgagg cgagggtgtg gtcgaccgga
     3361 tagcccgtgc ggggatcgat ggtgtgagca aagcgctcgc cgtcgacctc tagaaaattg
     3421 cgatagtcgc cggaagtggc aaggccagac ccgctgatcc gaagcacccg ctgaatcgcg
     3481 ccgaagctgt ccgggtccgg gacctccacg ccaattcgcc aggccgcgcc ctgagggttc
     3541 acgccggagg cgcgaacctc gccgccaacg tcgacgagaa aatcggaaca ttcaagctcg
     3601 aagagccttt gcgcaatccg gtc
//
LOCUS       DBNHQR010000494         3649 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_808497, whole genome shotgun sequence.
ACCESSION   DBNHQR010000494 DBNHQR010000000
VERSION     DBNHQR010000494.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_808497"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="AC16VR_11210"
     CDS             complement(<1..277)
                     /locus_tag="AC16VR_11210"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6011770.1"
                     /translation="MVPFPAPRFESVNGIRIACYEMGEGRPVVLCHGFPELAFSWHRV
                     MPRLADAGYRAIAIDQRGYGASDCPPAVEDYAITEIAADVAGLLDALG"
     gene            392..1366
                     /locus_tag="AC16VR_11215"
     CDS             392..1366
                     /locus_tag="AC16VR_11215"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6011771.1"
                     /translation="MTSNRFPDPAMIFTNGIRMTVYQDGPADGFPVVLCHGFPELAYS
                     WRHQLQALADAGFHAIAPDQRGYGRTDKPDDAGAYDIHHLTGDLAGLLDAMGLERAVF
                     CGHDWGGLVVWQMALLHPSRVAGVIGAGVPFLPRAEEDPVETFRRMWGERMYIVNFQT
                     SHEADELFDADPERVFQVLMRKNPMSLAEYAELPLQERVADLIARVKAGPSSTPSIIS
                     EEELRVFASAFRAGGFTGPINWYRNWSRNWSTTAHVPQKIEVPALYIKAGNDLATAAI
                     PDLESRMAGHVADLEIREVPESGHWLQQEFPDEVSGFTVDWLTRRFTS"
     gene            complement(1356..1604)
                     /locus_tag="AC16VR_11220"
     CDS             complement(1356..1604)
                     /locus_tag="AC16VR_11220"
                     /inference="COORDINATES: protein motif:HMM:NF021362.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HIR6011772.1"
                     /translation="MTASDLPQTESHLRSVAKAFSWRVVATLTTMTIAWMITGEVDTA
                     IMIGGFEFVLKIFIYYLHERAWQLVPRGAVRRIARLSS"
     gene            complement(1601..3499)
                     /gene="cysN"
                     /locus_tag="AC16VR_11225"
     CDS             complement(1601..3499)
                     /gene="cysN"
                     /locus_tag="AC16VR_11225"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008019847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HIR6011773.1"
                     /translation="MAHKSELIAGDIEAYLEAHQHKSLLRFITCGSVDDGKSTLIGRM
                     LYEAQLIFEDQVTSLKADSKRQGTQGGDIDFALLVDGLAAEREQGITIDVAYRFFNTD
                     RRKFIVADTPGHEQYTRNMVTGASTADVAVILVDASQGVLTQTRRHSFITSLLGIRHV
                     VLAVNKMDLVDYDRETFETITAAYEQFAEQLAFESITAIPLSALAGDNVIERSQRTSW
                     YSGPTLLGFLETVEVGRSQREHDLRFPVQWVNRPHSEFRGYSGTVVAGAVAVGDSVRV
                     LPSGEVAQVDGIVLHEDSLDQAVADQAVTITLDRELDVSRGDVIVAADAPCEVSDQFD
                     ASLVWMDQETGFIGRSYWLLMGTSRVNATLHDVKFKYNINTLEELPTRAFELNEIGRV
                     TLSLDKAVPFEAYQDCHSMGAFVLVDRYTHATVAAGMINFALRRARNIHRQAVTVDKQ
                     ARAKLNGHPPRVLWFTGLSGSGKSSITNAVEQVLHLQGVRTYILDGDNVRHGLNRDLG
                     FTETDRIENIRRIAEVAKLMVDAGVVVLTAFISPFRAERDMARSLFDKGEFLEIFLDV
                     PLEAAESRDPKGLYRKARRGELPNFTGIDSPYESPETADLTLNTAELSVDDCVSRVLE
                     LLKDQGVS"
     gene            complement(3499..>3649)
                     /locus_tag="AC16VR_11230"
     CDS             complement(3499..>3649)
                     /locus_tag="AC16VR_11230"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912238.1"
                     /note="with CysN catalyzes the formation of
                     adenylylsulfate from sulfate and ATP; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HIR6011774.1"
                     /translation="LTGAIESDADTLPAIIQEMLLTTTSERQGRVIDHDAAASMEKKK
                     QEGYF"
BASE COUNT          629 a         1194 c         1177 g          649 t
ORIGIN      
        1 cgccgagggc gtcgagcagg ccagccacgt ccgcggcgat ctcagtgatg gcgtagtctt
       61 ccaccgccgg cgggcagtca ctggccccgt aaccgcgctg atcgatggcg atggcgcgat
      121 agccggcatc cgccagcctc ggcatcaccc ggtgccatga gaacgcaagc tccggaaagc
      181 cgtggcagag caccacggga cgcccctccc ccatctcgta gcaggcgata cgaatgccgt
      241 tgacgctttc gaatcggggc gctggaaaag gaaccatcat ttcgtgccaa tcgtcgttcc
      301 gggtcgtcat cttaccctca cgaacgcccc ttgcgcctgg ccctgtctcg cgcgatggtt
      361 gggttcgcag cgctgacagc agagatgatc cgtgacgagc aaccggtttc ccgatcccgc
      421 gatgatcttc acgaacggca ttcgcatgac cgtctaccag gacggtcctg cggacggctt
      481 cccggtggtg ctctgccacg gctttccgga actcgcttac tcctggcgtc atcagctcca
      541 agcgctggcc gacgcgggct tccacgcgat cgcgcccgac cagcggggat acggccgcac
      601 ggacaagccc gatgacgcgg gggcgtacga catccaccac ctgaccggcg atctggccgg
      661 cctgctcgat gccatggggc tggagcgtgc cgtgttctgc gggcacgact ggggtgggct
      721 ggtggtctgg cagatggcgc ttctgcaccc gtcccgcgtc gccggcgtca ttggcgccgg
      781 agtaccgttt cttccgaggg cggaagagga ccccgtggaa acgttccgac gcatgtgggg
      841 cgaacgcatg tacatcgtga atttccagac gagccacgag gcagacgagc tgttcgacgc
      901 ggatccggag cgcgtcttcc aggttctgat gcgcaagaac ccgatgagcc tggccgaata
      961 cgccgagctt ccgctccagg agcgagtggc cgacctgatc gcgcgggtga aagctggacc
     1021 gtcgagcacg cccagcatca tctccgagga ggagctccgc gtcttcgcgt ccgctttccg
     1081 ggcaggcggg ttcaccgggc cgatcaactg gtaccgcaac tggagccgca actggtcaac
     1141 gaccgcccac gtgccgcaga agatcgaggt gcccgcactc tacatcaagg ccggcaacga
     1201 tctggcgacc gccgccatcc cggacctgga gtcacgcatg gccgggcacg ttgccgacct
     1261 ggagatccgc gaagtgcccg agagcgggca ctggcttcag caggaatttc cggacgaggt
     1321 gagcggtttc acggtggatt ggttgacccg gcgcttcacg agctgagacg cgcgatcctg
     1381 cgcaccgccc ctcgcggcac cagctgccac gcgcgctcgt gcaggtagta gatgaagatc
     1441 ttcagcacga actcgaagcc gccgatcatg atggcggtgt ccacctcgcc ggtgatcatc
     1501 caggcgatgg tcatggtggt gagcgtcgcc accacccgcc aggaaaaagc cttggcgacg
     1561 ctgcgcaggt gcgactcggt ttgcggtagg tcggacgctg tcatgaaacc ccctgatcct
     1621 tgagaagctc gaggacccgc gagacgcaat cgtccacgct gagctcggcc gtgttgagcg
     1681 tgaggtcggc cgtttccggc gactcgtaag ggctgtctat gccggtgaag ttcggcaact
     1741 ctccgcgacg ggctttacgg tagaggcctt tggggtcgcg cgactccgcc gcttccagcg
     1801 gaacgtccag gaagatctcc aggaactcgc ccttatcgaa cagtgaccgg gccatgtccc
     1861 gctccgcccg gaagggcgag atgaacgcgg tgagcaccac gacgcccgcg tccaccatga
     1921 gcttggcgac ctcggcaatc cggcggatgt tctcgatccg gtcggtttcg gtgaagccga
     1981 gatcccggtt cagcccgtgg cggacattgt ccccgtcgag gatgtacgtg cgcaccccct
     2041 gcaggtgcag gacctgctcc accgcattgg tgatgctgga tttcccggaa ccgctcaagc
     2101 ccgtgaacca gaggacgcgg ggcgggtggc cgttgagctt cgccctcgcc tgcttgtcga
     2161 ccgtcacggc ctgtcggtga atgttgcgag cccgacgcag ggcgaagttg atcatcccgg
     2221 cggcgacggt ggcgtgcgtg taccgatcga cgagcacgaa cgcgcccatg gagtgacagt
     2281 cctggtaagc ctcgaaggga acggccttgt ccaggctgag cgttacccga ccgatctcgt
     2341 tgagctcgaa agcccgggtc ggcagctcct cgagggtgtt gatgttgtac ttgaacttca
     2401 cgtcgtgcag cgtggcgttc acccgtgagg ttcccatcag caaccagtag gaacgtccga
     2461 tgaatcccgt ttcctgatcc atccacacca gggacgcatc gaactgatcc gagacctcgc
     2521 atggcgcatc cgcggccacg atcacgtcgc cccgggaaac gtcgagctcc cggtccagag
     2581 taatggtcac ggcctgatcg gccacggcct ggtccaggga atcctcgtgc aggacgatgc
     2641 cgtcgacctg tgccacctct ccggatggca gcacccgcac ggagtcgccg acggccaccg
     2701 cgcccgccac caccgtgccg gagtaaccgc gaaactccga gtgcggccgg ttcacccact
     2761 gcacgggaaa acgcaggtcg tgctcacgct gagagcgccc cacttccacg gtttccagaa
     2821 aacccagcag cgtcgggccg ctgtaccagg acgtccgctg cgaccgctcg atcacgttgt
     2881 cgcccgcaag cgcggacagc ggaatggcgg tgatcgactc gaaggcaagc tgctccgcaa
     2941 actgctcgta tgctgcggtg atggtttcga aggtctcccg gtcgtagtcc acgaggtcca
     3001 tcttgttcac ggccagcacc acgtgccgga tcccgaggag cgaggtgatg aaggagtggc
     3061 gccgggtctg ggtcagcacg ccctgggagg catccaccag gatcacggcg acgtccgcgg
     3121 tggaagcacc ggtgaccatg ttgcgcgtgt actgctcgtg gcccggcgtg tccgccacga
     3181 tgaacttgcg ccggtcggta ttgaagaagc ggtaggccac gtcgatggtg atgccctgct
     3241 cccgctccgc cgcaaggccg tccaccagca gggcgaagtc gatgtctccg ccctgggtcc
     3301 cctggcgctt ggagtcggct ttcagcgagg tcacctgatc ctcgaagatg agctgggcct
     3361 cgtagagcat gcggccgatg agcgtgctct tgccgtcgtc gacgctgccg caggtgatga
     3421 acctcagcag gctcttgtgc tggtgcgcct cgaggtaggc ctcgatgtcg cccgcgatca
     3481 gctcggactt gtgcgccatc agaagtagcc ttcctgcttc ttcttctcca tcgacgcagc
     3541 cgcgtcgtgg tcgatcacgc gtccctgccg ttccgaggtc gtcgtcagga gcatctcctg
     3601 gatgattgca ggcagcgtgt cggcgtcgct ctcgatcgcg ccggtcagc
//
LOCUS       DBNHQR010000495         8385 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1061485, whole genome shotgun sequence.
ACCESSION   DBNHQR010000495 DBNHQR010000000
VERSION     DBNHQR010000495.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8385)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8385)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8385
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1061485"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1214)
                     /gene="tig"
                     /locus_tag="AC16VR_11235"
     CDS             complement(<1..1214)
                     /gene="tig"
                     /locus_tag="AC16VR_11235"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIR6011775.1"
                     /translation="MSGLERRLTISVPSQTFEGQITERLNRAAQQVRLPGFRAGKVPM
                     KEVRRRFGPAVRAEVAGELMQSSFVEAVRQEELAPAGSPNLEVVKMSPGIDFEFTATF
                     EVFPKIELADLSGVHINRPNAQITELDVDEMVERLREQRKEWVAVDRGAEDGDRVTID
                     FAGRIDDEAFEGGSGEDVTFIVGAGQMIEDFDHGVRGMQAGTDGEFQATFPDDYRAEE
                     LRGKQATFSITVKEVTEGKLPELDDEFFAAFGIEEGGVDAFREDVRNNMQRELDNAVA
                     SQVKTQVMDQLNELHTVQLPKAVVKQEIEALKAQMLQQFQMPGTAAGGLPDLPDELFA
                     EQAQRRVAVGLIVSELVSRKSLEADADKVRARIQSIAEQYAEPQQVINWYYGNEDQLR
                     QIEMAVLEEQVI"
     gene            complement(1285..1360)
                     /locus_tag="AC16VR_11240"
     tRNA            complement(1285..1360)
                     /locus_tag="AC16VR_11240"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1325..1327),aa:His,seq:gtg)
     gene            complement(1434..1510)
                     /locus_tag="AC16VR_11245"
     tRNA            complement(1434..1510)
                     /locus_tag="AC16VR_11245"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1474..1476),aa:Arg,seq:tct)
     gene            complement(1527..1603)
                     /locus_tag="AC16VR_11250"
     tRNA            complement(1527..1603)
                     /locus_tag="AC16VR_11250"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1567..1569),aa:Pro,seq:tgg)
     gene            1773..2624
                     /gene="folD"
                     /locus_tag="AC16VR_11255"
     CDS             1773..2624
                     /gene="folD"
                     /locus_tag="AC16VR_11255"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010166832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HIR6011776.1"
                     /translation="MTASILDGKSLAKSLRADLAAAVQERLESGGRPPGLAVILVGND
                     PASEVYVRHKSRDCEEVGFHSVVQHLSENVTQQDLEERIVALNANPSIDGILVQLPLP
                     AHIDSHRIIDRIDPLKDVDGFHPYNIGRLALRRPVLRPGTPKGIMQLLEVTGVPIRGL
                     DATILGASNHVGRPMGLELLLAGCTVTTAHKFSRDARASARSADILVSAVGKPGLVGG
                     DWIKPGAIVIDVGITRGEDGKLRGDVVFDEAMEVASWVTPVPGGVGPMTRVAMLQNTL
                     AAADGSD"
     gene            2629..3345
                     /locus_tag="AC16VR_11260"
     CDS             2629..3345
                     /locus_tag="AC16VR_11260"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:TIGR01854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HIR6011777.1"
                     /translation="MHRIYISDLHLSDESEPQFATLAALLRHEAPRADAIFILGDLCE
                     AWVGDDDDSPLATALRAALKYAGEQTSLHVMRGNRDFLLGERFAAEIGCCLVEDPCLI
                     DPDTLIAHGDAFCIDDEAYQAMRRVLRSEAWQQDVLARPLEERRGLAAQMREQSRLSN
                     ANKAENIMDVSADEVDRTVKEYGARRLIHGHTHRPGVHPGDWGTRYVLGSWEHCGWLL
                     REQDTVTALECFPLSGRYGT"
     gene            complement(3299..3937)
                     /locus_tag="AC16VR_11265"
     CDS             complement(3299..3937)
                     /locus_tag="AC16VR_11265"
                     /EC_number="1.14.99.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007351798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045301 - tRNA
                     2-(methylsulfanyl)-N(6)-isopentenyladenosine(37)
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaE family protein"
                     /protein_id="HIR6011778.1"
                     /translation="MSATRSRAKPEDAAEFLKCSTPVLWFEQAAGNLETLLVDHANCE
                     KKAAGTALSLIYRYVDRPELLQRLSRLAREELRHFEQVHALMCSMGIAYHALAPGRYA
                     ASLMQQVRTHEPARLVDTLLIGAIVEARSCERFAGLVDVLPGGVGELYAALMVSEARH
                     FRGYLDLAQRYGDGSLEERLDHLLGFESELASGPDTQFRFHSGPIAGNIPAQ"
     gene            complement(3948..4394)
                     /locus_tag="AC16VR_11270"
     CDS             complement(3948..4394)
                     /locus_tag="AC16VR_11270"
                     /inference="COORDINATES: protein motif:HMM:NF018630.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HIR6011779.1"
                     /translation="MTATRRSTPCVGICSTTYGDLVCRGCKRFAHEIVSWNAFDVDQR
                     EAVWSRLEELRTGAIARCLRVLDEERLIAEAEAVRVPQAAQRELLDLAYEALRRWRDR
                     DAPPERLGVEALAGADTAQDLIRQIDREFYSRSLAHYERSFKIAAQ"
     gene            complement(4405..6426)
                     /locus_tag="AC16VR_11275"
     CDS             complement(4405..6426)
                     /locus_tag="AC16VR_11275"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF023418.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase TgpA family protein"
                     /protein_id="HIR6011780.1"
                     /translation="MASARASSGVGFAYQIPRNSLALLMFAQVVVVLPFALQLSPWII
                     AVGLFCGYWRAGVYQGRWSYPHRWVKALLVLASVVGVAVSGAGAFSLEAAASLLILAF
                     ALKLIEMKSRRDAYLVIFLAYFVIATQFLFDQSILVAVYELGAICVVTAAMIGLNQLH
                     TRVRPLASLRDASKLVLQALPFTLVLFLLFPRIAPLWTVPLPTAGKTGITDVVKPGAI
                     AALTQSDELAFRVVFEGEIPRYRDLYWRGLVYSEFEDGAWSVGPELPAVPSSLRPEPG
                     RGEVAYEVLLEPTMSDWLFALDTAFPRSAGVASTQDYRLRARDPILSVFRYGVASYPD
                     FVMDERLTGRLITRETRIDLQDSPRAVALARSLHDGDDERFVDAVLELIRQEPFRYTL
                     NPPRLPDAGSIDAFWFESRAGFCTHYAGAFVYLMRSVGIPARMVGGYQGGEINPISNH
                     LMVRQYDAHAWAEVWLRGKGWQRVDPTAAVAPERIERGLSAALSANDRARLSALTSAR
                     LGAWSTLADMLYWVDSLEHRWNLWVIGYDSRFQAGFLERLLGKVTPGRTGLVMLAGGV
                     ASIGLVAVFLFWRRRPRKRHPVERMFLRFAGKVSGYGLQRNPQESPGAFVRRAARNAG
                     WRDEQADQIVAQLNALLYNPAVSWGRAEVASLAAQLRRLQFRLAFASAR"
     gene            complement(6407..7375)
                     /locus_tag="AC16VR_11280"
     CDS             complement(6407..7375)
                     /locus_tag="AC16VR_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR6011781.1"
                     /translation="MADTAAHAFVGQRFGRWLDRRIPPGSRITLSQRNVFIFPTPTGF
                     VFGGLLILLVLGAINYQASLVYGVAFLLGSVFLMTILYTFRNLSGLSLELVSTRAGFV
                     GEAVEFSVRVVRPKGAGREGIQLGWPNGIKQWAELYEQEADTLRLYVKGERRGYLNPG
                     RLLVETYYPLGLLRAWTWVDLDARALVYPKPIFQDFPLSEQGRRDEGELIDPLGSEDF
                     TDIKGYQPGDPIKHIIWRSYARSNDVMVKRYASYLEPRLWLDLETVAGPLEERLSRLT
                     GMALTATRLEREFGLRLGGLEIKPATGETHLEQVLRELALHGIRES"
     gene            complement(7381..8355)
                     /locus_tag="AC16VR_11285"
     CDS             complement(7381..8355)
                     /locus_tag="AC16VR_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6011782.1"
                     /translation="MRKSTPIRRSSSYTTTPEAKDLIQALLSALNTVLLGKETQVKLA
                     LGCLFARGHLLIEDLPGMGKTLLSHALAKVLGLSYNRIQFTSDVLPGDIIGTSIFERD
                     TGGFRFIEGPLFAQLVLADEINRATPKSQSALLEAMEERQVTIEGKTRSLPEPFFVIA
                     TQNPTTQTGTFPLPESQLDRFLMRIELGYPEPDAERELLRGGDRRAVLEGLEPVSDLE
                     GLRALQRAVDEVKVSDLLIEYVQRLVAHSRRGSEFAFGLSPRGALALLHGARAWALIN
                     DRSHVVPEDVQAVLPSVVEHRLREAADFSGHGGGALAQKLLSSVDVVG"
BASE COUNT         1547 a         2790 c         2636 g         1412 t
ORIGIN      
        1 tcgatgacct gctcctctaa gacggccatc tcaatctggc gcaactggtc ttcgttgccg
       61 tagtaccagt tgatgacctg ctgcggctcg gcgtactgct cggcgatgct ctggatccgg
      121 gcccgcacct tgtcggcgtc ggcttccagg ctttttctgg acaccagctc gctgacgatg
      181 agaccaacgg caacccgacg ttgcgcctgc tcggcgaaca gctcgtcggg aaggtcaggc
      241 agaccgcccg cagcggtacc cggcatctga aactgctgca gcatctgggc cttgagcgct
      301 tcgatttcct gcttgaccac ggccttgggc aactggaccg tgtgaagctc gttgagctga
      361 tccattacct gggttttgac ctggctggca accgcgttgt cgagttcgcg ctgcatgttg
      421 ttgcgaacgt cttcccgaaa cgcgtccacg cctccttctt cgatgccgaa cgccgcaaaa
      481 aactcatcgt ccagctcggg aagcttgccc tcggtaactt ccttgacggt aatgctgaag
      541 gtcgcttgct tgccgcgcag ctcctcggcc cggtagtcgt caggaaacgt tgcctgaaac
      601 tcaccatcgg ttcccgcctg catgccgcgc acgccgtgat cgaaatcctc gatcatctgc
      661 cccgcgccca cgatgaaggt cacgtcttct cccgagccgc cctcgaatgc ctcgtcgtcg
      721 atgcgccccg caaaatcgat cgtgacccgg tcgccgtcct cagcaccgcg atccacggcc
      781 acccactcct tacgctgctc ccgaaggcgc tccaccatct cgtccacgtc gagctcggtg
      841 atctgggcgt tgggcctgtt gatgtgcacg ccgctcaaat ccgccagctc gatcttcgga
      901 aacacttcga aagtagcggt gaactcgaag tcgatacctg gcgacatttt cacgacttcg
      961 aggttgggcg acccggccgg cgccagttcc tcctgacgga cggcttccac gaagctcgac
     1021 tgcatcagct caccagccac ctccgccctt accgccggcc cgaaacggcg gcgcacctcc
     1081 ttcatgggca cctttccggc tctgaaaccc ggcagacgca cctgctgtgc ggctcggttc
     1141 agcctttcgg tgatctgacc ttcgaatgtc tgcgacggaa ccgagatggt caaccggcgc
     1201 tcgaggccgc tcatggtctc aacggaaata tgcatctgtc tgccgtgctt gccgctacct
     1261 ttaactgccg ccctggggtt gaaatggggt ggacgatggg gctcgaaccc acgaccgccg
     1321 ggatcacaac ccggagctct accaactgag ctacgtccac cgtagcctgg tctttcaccc
     1381 gaccgctggg cggcgccatt ctaaccgaac catcgagccg ctcgatacga gtctggtacg
     1441 cccggaagga ctcgaacctt caaccctcgg cttagaaggc cgatgctcta tccagttgag
     1501 ctacgggcgc attctcgatg agaaattggt cggggtagag agatttgaac tcccgacatc
     1561 ctgctcccaa agcaggcgcg ctaccagact gcgctatacc ccgtggctgt tgcacaaccc
     1621 gcgcccgaag cggcgcgatg atactggtgg tgttctgagc cgtcaaacgc agacgccggg
     1681 ccctgaacgg gtcgatcggc ggtcggggtt ggccggtctg aggccggttc gttgtaatat
     1741 gggggcctac aggcaagcgg aatccgggtg acgtgacagc ctccatcctc gacggcaaaa
     1801 gtctggcgaa atcgctcaga gcagacctgg cggcggcggt gcaggagcgg ctcgaatccg
     1861 gcgggagacc accgggcctt gcggtcattc tggtcggaaa cgatccagcc tcggaggtct
     1921 acgtgcgtca caagagtcgg gactgcgagg aagtcgggtt ccactccgtg gtgcagcatc
     1981 tgtccgagaa cgtcacccag caggacctcg aggaacgcat cgttgccctc aacgcaaacc
     2041 cgtccatcga tggcatactg gtccagctcc cgcttcccgc ccacatcgac agtcaccgga
     2101 tcatcgaccg gatcgaccct ctcaaggacg tggacggctt tcatccctac aacatcggcc
     2161 gtctcgccct gcgcagaccc gtgctgcgac ctggcacgcc aaaaggcatc atgcagctcc
     2221 tggaggtcac cggtgtcccg attcgcggcc tggacgcaac cattctcggc gcctccaatc
     2281 acgtcggccg accgatgggc ctggagctcc tgctggccgg atgcacggtg acgaccgcgc
     2341 acaaattctc ccgcgacgcc cgggcctcag cccggtcggc cgacatcctg gtgtctgccg
     2401 tgggcaagcc cgggctcgtc gggggcgact ggatcaaacc cggcgccatc gtgatcgacg
     2461 tcggcattac ccggggtgag gacggcaagc ttcggggaga cgtggtgttc gacgaggcga
     2521 tggaagtcgc cagctgggtc acgcccgtgc ccggcggcgt ggggcccatg acgcgggtgg
     2581 ccatgcttca gaacaccctc gccgcagccg acggatcgga ctgaaccctt gcaccggatc
     2641 tacatctcgg accttcatct ttcggatgaa agcgagccgc agttcgcaac gcttgcggct
     2701 ctgctgcggc acgaggcgcc tcgcgccgac gccattttca ttcttggcga cctgtgcgaa
     2761 gcgtgggttg gtgacgacga cgactcaccg ttggctaccg cgcttcgcgc agcgctgaag
     2821 tacgccggcg agcagaccag tctgcacgtc atgcgaggca acagggactt cctgctcggc
     2881 gagcggttcg cagcggaaat cggatgctgc ctggtcgagg atccctgtct gatcgacccc
     2941 gacaccctga tcgcccatgg ggacgccttc tgcatcgatg acgaggccta tcaggccatg
     3001 cgccgcgttc tgcgctccga ggcgtggcag caggacgtgc tggcacgccc gctggaggag
     3061 cggcgcgggc ttgctgccca gatgcgcgag cagagccgcc tgagcaacgc caacaaggca
     3121 gagaacatca tggacgtctc ggctgacgag gtggatcgga cggtcaagga gtacggggcc
     3181 agacggctaa tacatggtca tacccatcgc cccggcgttc acccggggga ctggggaacc
     3241 cgttacgtgc tcggctcctg ggagcattgc gggtggctgc tgcgcgagca ggacaccgtc
     3301 actgcgctgg aatgtttccc gctatcgggc cgctatggaa cctgaactga gtgtccggac
     3361 ctgacgcgag ctcggactcg aagcccaaca gatgatcgag cctctcctcc agcgaaccgt
     3421 cgccgtacct ctgcgctaga tccaggtatc ccctgaaatg gcgcgcttcc gagaccatca
     3481 gagccgcgta cagctcgccg accccgccag gaagcacgtc cacgagcccg gcaaaccgct
     3541 cacaggatcg ggcctccacg atggcgccaa tcaggagggt gtcaacgagc cgggccggct
     3601 cgtgggtcct gacctgctgc ataaggctgg ccgcgtagcg gccgggagcc agcgcgtgat
     3661 aggcgatgcc catggagcac atcagcgcgt gcacctgctc gaagtgacga agctcctccc
     3721 gggcgagacg ggacagccgc tgcagcagct cgggccgatc cacgtagcga tagatgagac
     3781 tgagcgcggt gcccgccgcc ttcttctcgc aattggcgtg gtcgacgagc agagtctcga
     3841 ggttcccggc cgcctgctcg aaccacagca ccggtgtcga gcacttgaga aactcggcgg
     3901 catcctcggg tttcgcgcgg cttcgggtgg cgctcacagc gtggcgctca ttgagcggcg
     3961 atcttgaagc tgcgctcgta gtgggcgagc gaccggctgt agaactcacg gtcgatctgg
     4021 cgaatcagat cctgcgccgt gtctgccccg gcaagggcct ccacgcccag ccgctccggg
     4081 ggcgcgtcgc gatcgcgcca cctcctgagc gcttcatagg ccaggtccag cagctctctc
     4141 tgcgcggcct gggggacccg cacggcttcg gcttcggcga tcagccgctc ctcgtcgagg
     4201 acgcgcaggc atcgggcgat ggcgccggtt cgcagctcct cgagacggct ccagacggcc
     4261 tcacgctggt ccacatcgaa ggcgttccag ctgacgatct cgtgggcgaa ccgcttgcac
     4321 ccgcggcaga ccaggtcgcc gtaggtcgtc gagcatatgc cgacacaggg ggtgctgcgg
     4381 cgcgtggccg tcatgacgat gtcgctacct cgcagatgca aaggccaatc tgaactgaag
     4441 ccgccggagc tgcgcggcaa gcgacgcgac ctctgcgcgg ccccaggaga cggccggatt
     4501 gtacagaagc gcattcaatt gggcgacgat ctgatccgcc tgctcgtccc gccatccggc
     4561 attgcgggct gcccggcgga cgaaagcacc cggcgactct tgggggtttc gctgcagacc
     4621 gtagccggaa acttttccgg caaagcgcag gaacatgcgc tcgaccgggt gccgtttgcg
     4681 ggggcgcctg cgccagaaga ggaagacggc gacgaggccg atgcttgcga caccgccggc
     4741 aagcatcacc agacccgtgc ggcctggcgt gaccttgccg agcaggcgct cgagaaatcc
     4801 ggcctggaag cgggaatcgt agccaatcac ccagaggttc cagcgatgct ccagcgaatc
     4861 tacccagtac agcatgtccg ccagggtgct ccaggcgccg agccttgcgc tcgtcagcgc
     4921 cgacagcctc gcgcgatcgt tggccgatag cgcagccgag agcccccgct cgatgcgctc
     4981 cggggccacg gccgccgtgg gatcgacgcg ctgccagcct ttgccgcgca accaaacttc
     5041 cgcccaggca tgagcgtcgt actgcctcac catgaggtga ttgctgatcg ggttgatctc
     5101 accgccctga tagccaccca ccatgcgcgc cgggatccca acggaccgca tgagatagac
     5161 gaacgccccc gcgtagtgcg tgcagaagcc ggcgcgagac tcgaaccaga acgcatcgat
     5221 actgcccgcg tcaggcagcc tgggagggtt cagcgtgtag cgaaagggct cctggcgaat
     5281 cagttcgagc acggcgtcga caaagcgctc atcgtcgccg tcgtgcagcg atctggccaa
     5341 cgccaccgct ctggggctgt cctggagatc gatcctcgtt tcacgcgtga tcaggcggcc
     5401 ggtcagccgc tcgtccatca cgaagtccgg ataggacgcc acgccgtatc ggaagacgga
     5461 caggatcggg tcgcgcgccc gcagccgata atcctgggtc gacgcaacgc cggccgaacg
     5521 cggaaatgcg gtgtccaggg cgaaaagcca gtcgctcatg gtcggctcga gcagaacctc
     5581 gtaggcgact tcgcccctcc cgggctcggg cctgagcgag ctcggcactg cgggcagctc
     5641 gggcccgacg gaccaggcgc cgtcctcgaa ttccgagtag acgagcccgc gccagtacag
     5701 gtcgcgataa cgcgggatct cgccttcgaa cacgaccctg aacgccagct cgtccgactg
     5761 ggtcagagcg gcgatcgcgc cgggcttgac cacgtccgtg atgcccgtct tccccgcggt
     5821 aggtagaggc accgtccaca gcggcgcgat gcgcggaaaa aggagaaaca gcaccagggt
     5881 gaaaggcagc gcctggagca ccagcttgga ggcgtcgcgc aacgaagcca gcgggcgcac
     5941 gcgcgtgtgc agctggttca ggccgatcat ggcggccgtg accacgcaga tggcccccag
     6001 ctcgtagacg gccaccagga tggactgatc gaagagaaac tgcgtggcga tcacgaagta
     6061 agcgagaaag atcaccagat aggcgtcgcg gcggctcttc atctcgatga gcttgagagc
     6121 aaacgccagg atgagcagcg aagccgccgc ctccaggctg aacgcccccg caccgctcac
     6181 ggccacgcca accacggacg ccagcaccag caaggctttc acccatcgat gcggatagct
     6241 ccaccggccc tggtagacgc ccgcgcgcca gtagccgcag aacaggccaa cggcaatgat
     6301 ccagggggag agctgcagcg caaagggcaa taccaccacg acctgcgcga acatcagcag
     6361 cgccaggctg ttgcggggaa tctggtaggc gaagcctacg ccggaactag ctctcgcgga
     6421 tgccatggag ggccagctcc ctgagcacct gctcgagatg cgtctcacct gtggcgggct
     6481 tgatttccaa cccccccagc ctcagaccga actcccgctc caggcgggtg gccgtcagtg
     6541 ccatgcccgt gagacggctc aaacgctctt ccagcgggcc ggcgacggtc tccagatcca
     6601 gccagagcct gggctcgaga tagctggcat accgcttcac catgacgtcg ttcgatcgcg
     6661 cgtacgagcg ccagatgatg tgctttatcg gatctccggg ctggtagccc tttatgtccg
     6721 tgaagtcctc gctgcccagg ggatcgatga gctcgccttc gtcccggcgc ccctgctccg
     6781 aaagagggaa gtcctggaag atgggcttgg gataaacgag ggcccgggcg tcgaggtcta
     6841 cccacgtcca cgcccgaagc aatccgaggg ggtagtaggt ctcgacgagc agccgcccgg
     6901 ggttgagata tcctcgtcgc tcacccttca cgtacaaccg cagggtatcg gcctcctgct
     6961 catagagctc ggcccactgc ttgatgccgt tcggccatcc cagctgaatc ccctcgcgcc
     7021 cggcgccttt tggacgcacc acgcgaacgc tgaactccac cgcctcgccg acgaacccgg
     7081 cgcgcgtgct gaccagctcc aggctgagcc ccgacagatt gcggaacgtg tagagaatgg
     7141 tcatcaggaa gacgctgccg agcaggaagg cgacgccgta gaccaggctc gcctgatagt
     7201 tgatggcgcc gaggacaagc aggatgagca gaccgccgaa aacgaacccc gtcggggtcg
     7261 gaaagatgaa aacgtttcgc tgactgagcg tgatacgcga accaggcggg atgcgtctgt
     7321 cgagccagcg cccgaaccgt tgcccaacga aggcgtgagc ggctgtgtcg gccacggatg
     7381 ctagcctacg acgtcaacgc tcgacagcag cttctgcgcg agcgcgccgc ccccgtgacc
     7441 cgagaagtcg gcagcttccc gcagccggtg ctccaccacg cttggcagca cggcctgcac
     7501 gtcttccgga accacatggc tgcgatcgtt gatgagcgcc caggcccggg cgccgtgcag
     7561 cagggccagg gcgccgcgag gcgaaagccc gaaggcgaac tccgatccac gacggctgtg
     7621 cgccaccagc cgctgcacgt actcgatcag cagatccgag accttcacct cgtccaccgc
     7681 tcgctgcagg gcgcgaagcc cttccaggtc cgaaaccggt tcgagcccct cgagcacggc
     7741 gcgccggtct ccccctcgca gaagctccct ctcagcatcg ggttccgggt acccgagctc
     7801 gatacgcatc aggaagcggt ccagctgaga ctcgggcagc gggaaggtgc ccgtctgagt
     7861 ggtggggttc tgagtcgcga tcacgaagaa cggttcgggc agcgaccggg tcttaccctc
     7921 gatggtgacc tgccgctcct ccatggcctc taggagcgcg ctctggctct tgggcgtcgc
     7981 gcggttgatc tcgtctgcaa ggaccagctg ggcaaagaga ggcccctcga tgaaacgaaa
     8041 cccgccggtg tcccgttcga agatggacgt tccgatgatg tcgcccggaa gcacgtcact
     8101 ggtgaactga atgcggttgt agctcaaccc gagcaccttc gcgagcgcgt gggagagcag
     8161 cgtcttgccc atcccgggca ggtcctcgat gagcagatga ccccgggcaa agaggcagcc
     8221 gagcgccagc ttgacctggg tctctttgcc cagaaggacg gtgttgagag cggacaggag
     8281 ggcctggatc aggtccttgg cctcaggcgt ggttgtgtag ctcgatgacc gtctgatcgg
     8341 cgttgatttc cgcatcccgg cgctgtttca tctggtcact tcgat
//
LOCUS       DBNHQR010000496         2970 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1631613, whole genome shotgun sequence.
ACCESSION   DBNHQR010000496 DBNHQR010000000
VERSION     DBNHQR010000496.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1631613"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="AC16VR_11290"
     CDS             <1..311
                     /locus_tag="AC16VR_11290"
                     /inference="COORDINATES: protein motif:HMM:NF044725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011783.1"
                     /translation="NSGGDRVYYNPETDYQITAGLTHEWRLMRRYQQSFVQRLSVDGG
                     EYHQKGFGGGPIWTLRLEHDWQIGRRVGMSWGATWGGRRYDGEREHVTTVFLTFRGHL
                     "
     gene            308..2242
                     /gene="pgaB"
                     /locus_tag="AC16VR_11295"
     CDS             308..2242
                     /gene="pgaB"
                     /locus_tag="AC16VR_11295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HIR6011784.1"
                     /translation="MRRRLAVIAAALALIVGAHGAGAESAHRIIAISYHDVVEHPSEV
                     GADRFTVTVDQLINQLAWLDAHGFEPITLDQWAAAAEGEPLPPRPVLLTFDDGYASFA
                     EHVMPVLKLFDFPAVLAPVTSWVDAPEGSVVQYGDQPMPRERFLTWDQLREISKTGLV
                     EIATHTHDMHRGLTGNQFGNELPAAVTRRWNDERNAYEPEADYEARIRSDLKRSRDRI
                     AEQLGKPPRAVVWPYGAYNLQAASVARTLGLTYTLTLDSLPNDPRIDGGRIHRELVST
                     DTSMLFFTTWALGKEQETPLRAAHVDMDYVYDPDPAQAERNLGRLLDRIKAMKLSFVF
                     LQAFADPDGDGVADALYFRNRHLPMRADLFNRVAWQLKTRAGVEVFAWMPVMAFELPD
                     AERNQALAVKAYDGSHESRYHRLSPYNPEARRIVGEIYDDLGRGSRFLGVLYHDDGFL
                     TDREDVSPAALARTVPDEAARGDGVLAPAVVSDFRGKTRFLIDFTHELSDVLRRHQPA
                     LLTARNLYARPVVDPQAEAWFAQSLGAFQRSYDYTAVMAMPYLEQAEEPEAWLSDLVE
                     KVRAIPGGMERTVFELQTVDWREGNPVRAATLANQMDLLLDRGVKHIAYYPDDFIQGY
                     PPVPLVRSRLSVNYHPALEE"
     gene            2247..>2970
                     /locus_tag="AC16VR_11300"
     CDS             2247..>2970
                     /locus_tag="AC16VR_11300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR6011785.1"
                     /translation="MTLDLFYRVLQEFALIYPLFMAYLWMVGGIYYLFHWERAGHARV
                     DRPPPLPEYPGVSVLVPAHNESFAIVETIRSLQNLKYPEYEIIVINDGSTDDTAEILD
                     ELAEEGGIRVIHLASNQGKAAALRVGAMASNHEFLVCIDGDAILDCHAVHWIMRHFLI
                     GPRVGAVTGNPRVRNRSTLLGKIQVAEFSSIIGLIKRAQRIYGRLFTVSGVVSAFRKT
                     ALYRVGFWDLTKLTDDIDISWRL"
BASE COUNT          507 a          962 c          966 g          535 t
ORIGIN      
        1 ggaactccgg cggcgaccgg gtctactaca acccggagac cgactaccag atcacggcgg
       61 gcctgaccca cgagtggcgt ctgatgcggc gctaccagca gtccttcgtt cagcggctga
      121 gtgtggacgg cggtgagtac caccagaagg gtttcggtgg cggcccgata tggaccttgc
      181 ggctcgaaca cgactggcag atcgggcgcc gggtgggcat gagctgggga gccacctggg
      241 gcgggcgccg ctacgacggc gagcgcgagc acgtgaccac cgtgttcctg actttccggg
      301 gacatctgtg aggcggcggc tggcagtcat cgccgccgcg ctggcgctca tcgtcggcgc
      361 gcatggtgcc ggtgccgaat cggcccatcg gatcatcgcg atctcctacc acgacgtggt
      421 ggagcatccc tctgaggtcg gggccgaccg cttcacagtg accgtagatc agctcatcaa
      481 tcagctggcc tggctcgacg cccacggctt cgagcccatc accctcgacc agtgggcggc
      541 tgcagccgag ggagagccgc tcccgccccg gccggtgctt ctcaccttcg acgacggcta
      601 cgcgagcttt gcagagcacg tgatgcccgt tctgaaactt ttcgattttc cggccgtgct
      661 cgcgccggtg accagctggg tggacgcccc cgaaggtagc gtcgtccagt acggcgacca
      721 gcccatgccg cgggagcgat ttctgacgtg ggatcagctc agagagatat cgaagaccgg
      781 cctcgtggag atcgcaacgc atacccacga catgcatcgt gggctcacgg gcaaccagtt
      841 cggcaacgag ttgcctgctg ctgtgactcg aaggtggaac gacgagagga acgcctacga
      901 gccggaggcc gattacgagg cgcgcatcag aagtgacctc aagcgcagcc gggaccgcat
      961 cgccgagcaa ctcggcaagc cgcctcgggc cgtggtgtgg ccctacggag cctacaacct
     1021 tcaggcggca agcgtcgccc ggaccctcgg gctcacgtac acgctgacgc tggacagcct
     1081 gccgaacgat ccgcgcatcg atggcggccg cattcatcgc gagctggtga gcaccgacac
     1141 gtcaatgctg tttttcacca cgtgggccct gggaaaggag caggagacac cgctgcgggc
     1201 agcgcacgtg gacatggatt acgtgtacga cccggacccg gcccaggccg aacgaaatct
     1261 cggccggctc ctggatcgta tcaaggcgat gaagttgtcc tttgtcttcc tgcaggcctt
     1321 cgcagatccc gacggcgacg gcgtcgcgga cgcgctgtac ttccgcaaca ggcacctgcc
     1381 catgcgtgcc gatcttttca accgggtggc ctggcagctg aaaaccaggg cgggcgtgga
     1441 ggtgtttgcc tggatgccgg tcatggcgtt cgagctgcct gatgccgaac gcaatcaagc
     1501 gcttgcggtg aaggcctatg acgggtccca cgagtcccgc taccaccggc tgtctccgta
     1561 caacccggag gcccggcgca tcgtggggga gatctatgac gatctcggtc gcggcagccg
     1621 ctttctgggc gtgctctacc acgatgatgg gttcctgacc gatcgcgagg acgtcagccc
     1681 ggccgctctc gcccgcaccg tccccgacga ggcagcgaga ggcgacggcg ttctggcgcc
     1741 ggcggtcgtg agcgacttcc gcggcaaaac ccgattcctc atcgatttca ctcacgagct
     1801 ttcggacgtg ctgcgtcggc accagcccgc gctgctcacc gcgcgaaacc tgtacgcgag
     1861 gccggtcgtg gatccccagg ccgaggcctg gtttgctcaa agcctcggcg cttttcagcg
     1921 tagctacgac tacacggccg tcatggccat gccctatctc gagcaggcgg aggagccgga
     1981 ggcgtggctt tccgacctgg tggagaaggt gcgggcgatc cccggcggca tggagcggac
     2041 ggtgttcgag ctccagaccg tcgactggcg cgagggcaac cccgtgcggg cggcgacgct
     2101 ggcgaaccag atggatcttc tgctggaccg cggcgtgaag cacatcgcct actatcccga
     2161 cgacttcatc cagggctacc cgccggtgcc cctcgtgcgc tcgcgcctgt ccgtcaacta
     2221 ccacccggcg ctggaggagt agtggcgtga cgctggatct tttctatcga gttctccagg
     2281 agtttgcgct catctacccg ctgttcatgg cctatctgtg gatggtgggc ggtatctact
     2341 acctctttca ctgggagcgt gccgggcacg cccgcgtgga ccgtccgccg ccgcttcccg
     2401 agtaccccgg tgtttccgtt ctggtgcccg cgcacaacga gtcgttcgcc atcgtcgaga
     2461 ccatccggtc gctgcagaac ctcaagtatc ccgaatacga gattatcgtc atcaacgacg
     2521 gcagcaccga cgacactgcc gagattctcg acgagctggc ggaggaggga ggcatccggg
     2581 tcattcatct tgcctccaac cagggcaagg cggccgcgct gcgggtgggt gccatggcct
     2641 cgaatcacga gtttctggtc tgcatcgacg gggacgccat tctcgattgc catgccgtcc
     2701 actggatcat gcggcacttt ctgattggtc ccagagtagg ggcggtgacc ggcaacccgc
     2761 gggtcaggaa ccgctccacg ctgctgggta agattcaggt ggccgagttc tcgtccatca
     2821 tcgggctgat caagcgggcc cagcgcatct acgggcgtct gttcacagtc tcgggcgtgg
     2881 tttcggcgtt tcgaaagacg gctctctatc gcgtcgggtt ctgggacctg accaagctca
     2941 ccgacgacat cgacatcagc tggcggctgc
//
LOCUS       DBNHQR010000497         2489 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1316249, whole genome shotgun sequence.
ACCESSION   DBNHQR010000497 DBNHQR010000000
VERSION     DBNHQR010000497.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1316249"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..794)
                     /locus_tag="AC16VR_11305"
     CDS             complement(3..794)
                     /locus_tag="AC16VR_11305"
                     /inference="COORDINATES: protein motif:HMM:NF024762.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HIR6011786.1"
                     /translation="MAGLAEQELIQAVRAFASDPEADELEAAVLVNRALEPSLDADTL
                     RGRIAELAAACPETQTPWDYLRELGFVGNAANYDALDNSRLDRLLDTRRGIPIALGVL
                     LIEVARASGRMAVGVNFPGHFLVRVGESLVDPFRMTQTSEDECVAGLGQARGNPGAGG
                     DLFPSATAAAVLLRMLNNIKYRYAQAGEWHRSLDMVDLQLAAAGESAHLLLERGELWL
                     RLGARGPARADLERALALAGDGDPRLAAAARRLLDGLGSDPELLH"
     gene            complement(795..2390)
                     /locus_tag="AC16VR_11310"
     CDS             complement(795..2390)
                     /locus_tag="AC16VR_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003379714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HIR6011787.1"
                     /translation="MVATARTAALAGGSDRRTFAIISHPDAGKTTITEKLLLFGNAIQ
                     LAGTVKARKSDRHATSDWMAMEQQRGISVTTSVMQFPYRDRVVNLLDTPGHEDFSEDT
                     YRTLTAVDSAIMVIDGSKGVEPRTIKLMEVCRLRDTPILTFINKLDREIRDPVEVMDE
                     IEDILQIQCAPMTWPIGMGRQFKGIYDLYADEIVCFVQGHGHHIFDYPRIKGLESNAA
                     RELLGSDHDDVVEEIELVRGASHAFDVDDYLGATLSPVYFGTALGNFGVKEMLDGFVT
                     YAPPPQARHADARDVKPGEEQFSGFVFKIQANMDPKHRDRIAFLRVCSGRYAQGMRMR
                     HVRLGKDVKVADAVTFLAGDRAQTVEAFPGDIIGLHNHGTIQIGDSFTQGEVIKFTGI
                     PNFAPELFRRVRVKDPLRSKQLERGLDELSEEGASQVFKPLIRNELIVGAVGMLQFDV
                     VAFRLREEYGADCVYDSTNIFAARWVVSDDKKKLEDFTRKHRDQLAVDGGGHLAYLAP
                     TRANLQLVEERWPEIRFLKTREH"
BASE COUNT          449 a          829 c          775 g          436 t
ORIGIN      
        1 aatcagtgca gcagctccgg gtcggacccc agcccgtcga gcaaccgtcg agccgcggcg
       61 gccaagcggg gatcgccgtc cccagcaaga gccaacgcgc gctccagatc ggcacgtgcc
      121 ggtcccctgg cgcctagccg caaccacagc tcaccgcgtt cgagaagcag atgcgcgctc
      181 tcccccgcgg ccgctagctg cagatccacc atgtcgaggc tgcgatgcca ctcccccgct
      241 tgggcgtagc ggtacttgat gttgttgagc atacgcagca ggacggccgc cgcggtggcc
      301 gaaggaaaga gatcgccccc cgcgcccggg ttccctcgcg cctggccgag accggcaacg
      361 cattcgtcct cggaggtctg cgtcatccgg aacggatcta ccagactttc tcccacccga
      421 accagaaagt gcccgggaaa gttgacgccg acggccatgc ggccgctggc gcgcgccacc
      481 tcgatcagca ggacgcccag ggcgatgggt atgccccgcc gggtgtcgag cagacggtcc
      541 agccggctgt tgtcgagcgc gtcgtaattc gcggcattgc cgacgaatcc aagctcccgg
      601 aggtaatccc agggtgtctg cgtttccgga caggccgctg caagctcggc aatgcgccca
      661 cgcagggtgt ccgcatccag agacggctcc agagcgcgat tgacgagcac ggccgcttcg
      721 agctcgtcgg cttcagggtc cgaggcaaac gctctgaccg cctgaatcag ttcctgctct
      781 gcgaggccgg ccaatcaatg ctcccgggtc ttgagaaagc ggatctcggg ccagcgctcc
      841 tccaccagct gcaggttcgc ccgggtcgga gcaaggtaag caaggtgtcc tccgccatcc
      901 acggccagct gatcgcggtg cttgcgcgtg aagtcttcga gctttttctt gtcgtccgag
      961 accacccagc gcgcagcaaa gatgttggtc gaatcgtaga cacagtcggc cccgtactct
     1021 tcgcgcagac ggaacgccac cacgtcgaac tgcagcatgc ccaccgcacc aacgatgagc
     1081 tcgttgcgga tcaagggctt gaaaacctga ctggctccct cctccgacag ctcgtccagc
     1141 ccccgctcca gctgtttgct cctgagcgga tcctttaccc gcacccgtcg gaacagctcg
     1201 ggcgcaaagt tcgggatccc ggtgaacttg atcacctcac cctgggtgaa ggaatcgccg
     1261 atctgaatgg tgccgtgatt gtgaagcccg atgatgtcgc caggaaacgc ctccaccgtc
     1321 tgggcccggt caccggcgag aaaggtcacc gcatcggcaa ccttgacgtc ctttccgagc
     1381 cgcacgtggc gcatccgcat gccctgggca taacggcccg agcacacccg gaggaaggcg
     1441 atgcgatcgc ggtgcttcgg gtccatgttc gcctggatct tgaacacgaa cccgctgaac
     1501 tgttcctccc ccggcttcac gtcgcgggcg tcggcgtgtc gggcctgggg gggcggggcg
     1561 tacgtgacga acccgtcgag catctctttg actccgaagt tgccgagcgc ggtcccgaag
     1621 tagacgggcg acagcgtcgc gccgagatag tcgtcgacgt cgaacgcgtg gctggccccg
     1681 cgtaccagct cgatctcttc gaccacatca tcgtggtccg aacccagcag ctcgcgcgcg
     1741 gcgttgctct ccaggccctt gatccgcggg tagtcgaaaa tgtgatggcc gtgcccctga
     1801 acgaagcaga cgatctcatc ggcgtacaga tcgtagatgc ccttgaactg gcggcccatg
     1861 ccgatcggcc aggtcatggg cgcacactga atctgcagga tgtcctctat ctcgtccatc
     1921 acctccaccg ggtcccggat ctcccggtcg agtttgttga tgaacgtgag aatcggcgta
     1981 tcgcgcagcc ggcagacttc catgagcttg atcgtgcgcg gctcgacgcc cttggagccg
     2041 tcgatgacca tgatggcgga atcgaccgcg gtgagcgttc gataggtatc ttcggagaag
     2101 tcctcgtgcc ccggggtatc gagcaggttc accacccggt cccgataggg aaactgcatc
     2161 accgaggtgg tgacggaaat cccccgctgc tgttccatgg ccatccagtc ggaggtcgca
     2221 tggcggtccg acttgcgcgc tttcaccgtg ccggcgagct ggatggcgtt gccgaacagg
     2281 agcagcttct cggtgatcgt ggtcttgccg gcatccggat gagagatgat cgcaaaggtg
     2341 cgtcgatcgc tcccccccgc gagcgcggcg gtccgggccg ttgccaccat gataaagact
     2401 cctcagcgac cgacttttcc gaagccggac ggccccgccg gaaaacggcg cggagtatag
     2461 cggggcgctg cggaaatgag catcaagat
//
LOCUS       DBNHQR010000498         3992 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_555141, whole genome shotgun sequence.
ACCESSION   DBNHQR010000498 DBNHQR010000000
VERSION     DBNHQR010000498.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3992)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_555141"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1890
                     /gene="metG"
                     /locus_tag="AC16VR_11315"
     CDS             <1..1890
                     /gene="metG"
                     /locus_tag="AC16VR_11315"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012613760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HIR6011788.1"
                     /translation="YVCADDTHGTATMLKAEEEGVEPERLIERIRAEHARDFQGFLVA
                     HDNYYSTHSQENRHYAELIYQRLKDGGLIFTREVAQLYDPQRKLFLADRFVKGRCPRC
                     DAEDQYGDNCEACGATYDATELKDPRSLISGAVPVLERSEHHFFDLPQYTEMLKAWTA
                     GGAVQPEVQNKLAEWLDAGLKPWDISRDAPYFGFLIPGTQDKYFYVWMDAPIGYMASF
                     QNYVSERKDVDFDTYWEPEGDAEVHHFIGKDIVNFHALFWPAVLEGAGFRKPTRLHVH
                     GFVTVEGRKMSKSRGTFVNASTYLEHLNPEFLRYYYATKLNGTIDDMDINLDDFVQRV
                     NSDLVGKVVNIASRCAGFITKQFDGRLAGTLHDAELQGRFVDAAAPIAELFEAGDTSK
                     AVREITALADQANQYIAHHAPWSMIKEAGREAEVQAVCSQGINLFRILAIYLKPILPR
                     MASEAEAFLEIEPLAWKDVDAPLLDHPIGAFRPLFTRMERKAVDRMIEAGREAGSEPV
                     PDSAPSSEAPAESHQISIDDFARIDLRVAKISAAEPVAGADKLLRLTLDLGDSNRQVF
                     AGIKSAYEAQSLQGRLCVVVANLAPRKMRFGVSEGMVLAAGPGGKDIFLLSPDSGAEP
                     GMIVR"
     gene            1897..2475
                     /gene="rsxA"
                     /locus_tag="AC16VR_11320"
     CDS             1897..2475
                     /gene="rsxA"
                     /locus_tag="AC16VR_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HIR6011789.1"
                     /translation="MAELALIVVGALLVNNFVLAQFLGLCPFIGVSGNYDTAVAMGFA
                     TTFVLTLAAAVSYMAYHWLLLPLGLEFLRVIVFIVVIAALVQLTEVFLRNTSPVLHNA
                     LGIYLPLITTNCAVLGVALLAINHSLSLLGTVAYAIGAAVGFTMVLVVFAAMRERLAH
                     AEVPAAFRGAPITLISAGILSLAFLGFAGLGN"
     gene            2495..3409
                     /locus_tag="AC16VR_11325"
     CDS             2495..3409
                     /locus_tag="AC16VR_11325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HIR6011790.1"
                     /translation="MLTALVLTAVLTAVVAALGAALVWADRRYPPDTRTLVESIDGLL
                     PQTQCAQCGYPGCRPYAEAVAAGAAINLCPPGGPETHRALARLLGRSEEDPPQEASKL
                     KARIREDGCIGCYLCVEACPVDAIVGAPRYLHTVLEAACTGCELCVPACPVDCIDMTP
                     AREASPISHQPRRRWPDRQSSPCIRCGACQPACPVGLSPQELLWYAGGSSSGRPTGQA
                     AAATRGLDRCIECGLCNPVCPSNIDLVGVFRQARRAQAARHEAERDAADAKARHARRT
                     ERLEETTAKRAEDREQRIARRSGRQWRA"
     gene            3397..>3992
                     /locus_tag="AC16VR_11330"
     CDS             3397..>3992
                     /locus_tag="AC16VR_11330"
                     /inference="COORDINATES: protein motif:HMM:NF015100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HIR6011791.1"
                     /translation="MAGLSSRGIMMAVCLALLPGTATMIYFYGVGVVHNLLLAVPVGL
                     AVEAICLQLQQRSVGRTLADGSTVLTCVLIALALPPGAPFEILSIAVVAAVALAKHLY
                     GGLGSNLFNPALVGYAVVLVSFPVAMASWPIPMDGETGATALVSMKYREGLTVAEAWQ
                     GARGFGAIGGYGWEWINLGFLLGGAVLVWRRLAAWRVPA"
BASE COUNT          645 a         1362 c         1310 g          675 t
ORIGIN      
        1 tacgtctgcg cggacgatac ccacggcacg gccaccatgc tcaaggccga ggaagaggga
       61 gtcgagcccg aacggctcat cgagcggatc cgcgccgagc atgccaggga cttccagggc
      121 tttctcgtgg cccacgacaa ctactactcc acccattccc aggaaaaccg ccattacgcc
      181 gagctcatct accagcgcct gaaggacggc ggcctgatct tcacccggga ggtggcccag
      241 ctctacgacc cccagcggaa gctgtttctc gccgaccgtt tcgtcaaggg ccgctgcccg
      301 cgctgcgacg ccgaggatca gtacggcgac aactgcgaag cgtgcggcgc aacctacgac
      361 gccaccgagc tgaaggatcc gcgctcgttg atctccgggg cggtccccgt gctggagcgc
      421 tcagagcacc acttcttcga tcttccccag tacacggaaa tgctgaaagc gtggacggcc
      481 ggcggcgcgg tgcagcccga ggtgcagaac aagctggccg agtggctcga cgcgggcttg
      541 aagccctggg acatttcccg ggacgcgccg tacttcggct ttctgattcc agggactcag
      601 gacaagtact tctacgtgtg gatggacgcg cccatcggct acatggcgag cttccagaat
      661 tacgtctccg agcgcaaaga cgtcgatttc gacacgtact gggagccgga gggcgacgcc
      721 gaggtacatc atttcatcgg caaggacatc gtcaactttc acgcgctgtt ctggccggcg
      781 gtgctggagg gcgccgggtt ccgcaagccc acccgtctgc acgttcacgg ctttgtcacc
      841 gtcgaagggc gcaagatgtc gaaatcgcga ggcacgttcg tcaacgcgtc cacctacctc
      901 gagcacctca accccgagtt cctgcgctac tactacgcga ccaagctgaa cggaaccatc
      961 gacgacatgg acatcaatct ggacgacttc gtccagcgcg taaactccga tctggtcggt
     1021 aaggtcgtca acatcgccag ccgctgtgcc gggttcatca ccaagcagtt cgacgggcgc
     1081 ctggcgggca cgctgcacga cgccgagctg caggggcggt tcgtcgacgc cgccgcgccc
     1141 atcgccgagc tcttcgaagc cggcgacacc agcaaggccg tgagggaaat cacggcgctg
     1201 gccgaccagg cgaaccagta catcgcccac cacgcgccct ggtccatgat caaggaagcg
     1261 ggccgagaag ccgaggtcca ggcggtgtgc agccagggca tcaacctgtt ccgcatcctg
     1321 gcgatctacc tcaagccgat cctgccgcgg atggcgagcg aagccgaagc cttcctcgag
     1381 atcgaaccgc tcgcctggaa ggatgtggat gcgcctctgc tcgatcaccc gatcggcgcg
     1441 ttcaggcctc tgttcacgag aatggagagg aaggccgtgg accggatgat cgaggcgggc
     1501 cgggaggcgg gctccgagcc cgtcccggac tccgccccat ccagcgaggc gcccgccgaa
     1561 tcacatcaga tcagcatcga cgactttgcc aggatcgacc tgagagtggc gaagatcagc
     1621 gccgcggagc ccgtggcagg cgccgacaag cttctgcggc tgactctgga tctcggcgat
     1681 tccaaccgcc aggtcttcgc cggtatcaag tctgcctacg aagcacagag tctgcagggc
     1741 aggctgtgcg tcgtggttgc caacctcgct ccgcgcaaga tgcgcttcgg ggtatccgag
     1801 gggatggtgc tcgccgccgg cccgggcgga aaggacatct tcctgctgag cccggactcg
     1861 ggcgccgagc cgggcatgat cgtgcgctga gccgccatgg ctgagctcgc tctcattgtc
     1921 gtcggcgctc tgctcgtcaa caatttcgtg ctggcccagt tcctcggcct gtgcccgttc
     1981 atcggcgtgt ccggcaacta cgacacggcg gtggccatgg ggttcgccac caccttcgtg
     2041 ctgaccctgg cggccgccgt gagctacatg gcctatcact ggctgctgct gcctttgggg
     2101 ctggagtttc tgcgggtcat cgtgttcatc gtggtcatcg ctgccctcgt ccagctgacg
     2161 gaggtttttc tgcgcaatac gagccccgtt ctgcacaacg ccctgggcat ctacctgccg
     2221 ctgatcacca cgaactgcgc ggttctgggc gtcgccctgc tggccatcaa ccacagcctc
     2281 tccctgctgg gaaccgtggc gtacgccatc ggggcggcgg tgggtttcac gatggttctg
     2341 gtggtcttcg cggccatgcg ggaacgcctc gcccacgccg aagtgccggc ggcatttcgc
     2401 ggtgcgccca ttaccctgat cagcgcggga attctctctc ttgcgttcct cgggttcgcg
     2461 gggctcggca actgatgggc agcgcaggtc ccccatgctg accgctttgg tactgactgc
     2521 cgtgctgacc gccgtggtgg ccgccctggg ggccgcgctc gtctgggcgg accggcgcta
     2581 cccgccggat acgcggacgc tggtggagtc gatcgacggt ctgctccccc agacccagtg
     2641 cgcgcagtgc ggataccccg gctgccgacc ctacgccgag gccgtcgccg cgggcgcggc
     2701 catcaacctg tgccctccag gcggtcccga gacacaccgg gccctcgccc ggttgctcgg
     2761 ccgcagcgaa gaagacccac cgcaggaggc ttccaagctc aaagcccgca tccgagagga
     2821 cggctgcatc ggctgttacc tgtgcgtgga ggcatgcccc gtggacgcca tcgtgggcgc
     2881 gccgcgctat cttcacacgg tcctcgaggc ggcgtgcacg ggctgcgagc tttgcgtccc
     2941 ggcgtgcccg gtggactgca tcgacatgac gcccgcccgg gaggcttccc ccatcagcca
     3001 tcagccgcgc cggcgatggc cggaccggca gagctcacca tgcatcaggt gcggagcctg
     3061 ccagcccgcc tgcccggtgg ggctgagccc ccaggagctg ctctggtacg ccgggggctc
     3121 ctccagcggg aggcctaccg gtcaggcggc cgccgcgacc cgcggcctgg atcgctgcat
     3181 cgagtgcggg ctttgcaacc cggtctgtcc cagcaacatc gacctcgtgg gcgtctttcg
     3241 ccaagcgcga cgagcgcagg ccgcaaggca cgaagccgaa cgggacgccg ccgatgccaa
     3301 ggcgcggcat gcaagacgca ccgaacgcct cgaggagacg accgccaagc gcgctgagga
     3361 tcgcgagcag cgcattgccc gacggagcgg caggcaatgg cgggcctgag ctcccgcggg
     3421 atcatgatgg ccgtctgcct ggcgcttttg ccgggcaccg cgacgatgat ctatttctac
     3481 ggggtcggcg tggtgcacaa cctgctgctc gccgtgcccg tcgggctggc cgtcgaagcc
     3541 atctgcctgc agttgcagca gcgttccgtc ggccggacgc tggccgatgg cagcacggta
     3601 ctcacgtgcg tgctcatcgc gctcgccctg ccgcccggcg cgccctttga aattctctcc
     3661 atcgccgtcg ttgcggccgt agccctggca aaacaccttt acggcgggct ggggagcaac
     3721 ctgttcaacc ccgcgctcgt ggggtacgcc gtcgtcctgg tctcgtttcc cgtggcgatg
     3781 gcgagctggc ccatcccgat ggacggcgaa accggcgcca cggcgctggt gtcgatgaag
     3841 taccgggagg ggctcaccgt cgcggaagcg tggcagggcg cacgcggctt cggcgccatc
     3901 ggcggctacg gctgggagtg gatcaacctg ggcttcctcc taggcggggc tgtgctcgtg
     3961 tggcggcggc tggcggcatg gcgcgtgccg gc
//
LOCUS       DBNHQR010000499         5815 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1187642, whole genome shotgun sequence.
ACCESSION   DBNHQR010000499 DBNHQR010000000
VERSION     DBNHQR010000499.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5815)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5815)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5815
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1187642"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="AC16VR_11335"
     CDS             complement(<1..435)
                     /locus_tag="AC16VR_11335"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6011792.1"
                     /translation="MIFRQLFDADSSTYTYLLGCADTRDAVIIDPVFEQHGRDCALIR
                     ELGLKVRYVLDTHVHADHVTSAWLMHSSLDAEIVLSRRYGIEGVDVAVDHGDVLAFGN
                     CSLTVRATPGHTSGCVTYVTNGQDMAFTGDCLMIRGAGRTDFQ"
     gene            501..1670
                     /locus_tag="AC16VR_11340"
     CDS             501..1670
                     /locus_tag="AC16VR_11340"
                     /inference="COORDINATES: protein motif:HMM:NF013624.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I family protein"
                     /protein_id="HIR6011793.1"
                     /translation="MYTPFAAATERVPNFAASGLIIRSQGPSAPDPETQRLPRAIASL
                     PGVADRHLNLAKQLERQLARFTDAGQADTAVAESARTLWRAAVSDVQNGLDDDRPLYW
                     GRLALRRSVYRSALREKDSLLRSIEHYSRGMGDLRLGDDVLNVLITGFDPFHLETRLD
                     QSNPSGLAALALNGSEFGSGGGRARVCASIFPVRFEDFDDGIVERFFQQILAEREPAM
                     VVTLSMGRDAFDLERFPGRRRSAQDPDNHNVLTGASPDKPLPPGKTGSHLEGPEFLEF
                     SLPVHAMRAADGHWPVRDNRWVTTIERGEFEAGSLADLQSQLAVRGSGGGYLSNEIAY
                     RSRLLHGQLGCSCPIGHVHTPAIQGHNAALERLMVGQIRQMLAQAAASLTPRPPS"
     gene            complement(1667..2689)
                     /locus_tag="AC16VR_11345"
     CDS             complement(1667..2689)
                     /locus_tag="AC16VR_11345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019471932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIR6011794.1"
                     /translation="MMNVNRQWVLAKRPYGMVTKDNFEYREETIPEPGDGEVLIRNLY
                     LSFDPTQRGWMEDRESYLPPVQIGEVMRAGSVGQVVESRSDDFSAGDLVQTTGGWQDF
                     TVMSAGMGPMGLRKLPEGVTPRLALGALGVTGLTAYWGMLDLGQPKAGETVLVSGAAG
                     ATGSVAGQIARIKGCRVVGIAGGPEKCAWLKDEARFDDVIDYKNEDVDRRIGELCPNR
                     VDVFFDNVGGGILEAALNHINMRARVVLCGGISAYNATEPQPGPVNLMNLVIMRARME
                     GFIVIDYMDRSHIAVPELLGWINSGEIVHQEDVQEGFENIPDTLNRLFTGRNLGKQLL
                     KIADPS"
     gene            2768..4222
                     /locus_tag="AC16VR_11350"
     CDS             2768..4222
                     /locus_tag="AC16VR_11350"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF004816.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR6011795.1"
                     /translation="MSDIAFLSATELARRIRDRELSSEELLLHYLQRVDRYNGALNAI
                     VVDIREEALMAARAADAAVARGVDVPPFHGVPMTVKESYNVAGTPTTWGNPKWRGNVA
                     FEDAESVKRLKDAGVIIFGKTNVPLALADFQSFNAIYGTTNNPFDHSRAPGGSSGGSA
                     AALAAGLTGLEAGSDIGGSIRNPAHFCGVFGHKPTWNLLWMRGHAPPGDVRASSDISV
                     IGPLGRSAADLETAVRTMAGPDDIMARGYTLSLPTLKRGGLGGLRIAIWEDDPVCPVS
                     AETKARVLAVASACRDAGAEIIEGARPDFSSEHSHETYQNLLQPTMASRMPDEEYESL
                     LEYVANLDPRDTSRTARVLRAQVARFRDWKRSHELREHLRWKWHLFFQDYDLMLTPMM
                     PTSAFPHDHRPISQRTIEVDGTSMPYMDQIFWAGLTGVAYLPSTVIPTGLDDSGLPIG
                     VQLVGPEYGDLITIGVARELETAGFRFVAPPAYA"
     gene            4235..5179
                     /gene="mvaD"
                     /locus_tag="AC16VR_11355"
     CDS             4235..5179
                     /gene="mvaD"
                     /locus_tag="AC16VR_11355"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018402752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HIR6011796.1"
                     /translation="MGTTARAHPNIALIKYWGKAPGQENIPATPSLSITLDSLVTETH
                     VATGPSRDELYFDDEPVADRKVLDWLEVLRQRYDIPPLEIRTANNFPTAAGLASSASG
                     FAALAVALDAQCGLQLDAATLSDLARRASGSAARSIFGGYVAVQGPRWVAQPVLSPEA
                     WPLKTVIAVTTTARKSVSSSEGMAISAATSPYYPGWVESTRADFTDALAAVRTRDFQA
                     LAELAEFSCLKMHGLMLGSRPGLLYWKAATVSCLHAVRALRQDGVDVFFTVDAGPQVK
                     AICKPSAESDVRAALAAVPGVEQIVVTGLGSGARVIDP"
     gene            5176..>5815
                     /locus_tag="AC16VR_11360"
     CDS             5176..>5815
                     /locus_tag="AC16VR_11360"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012509.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase family protein"
                     /protein_id="HIR6011797.1"
                     /translation="MIHASAPGKVVLWGEYAVLTGAPGLVLAVDRRASCTLTPGGDAW
                     HFETRGLPEPAVDLDVADLTGAHPPGPSGGALAWHVLRHLDSEVPAGATLTTDTASFQ
                     DGGHKLGIGSSAAVCTAVYGAFCELLEIGPSLNDALAVHRSLQGGSGSGIDVAAAYLG
                     GSLRYQLRGGARPPAAEPFDLPDGLILRFIWTGTPAQTTRHVARFEAWRAENQ"
BASE COUNT         1000 a         1981 c         1844 g          990 t
ORIGIN      
        1 ctgaaaatcg gtgcggcctg cgccccggat catcaggcaa tcgcccgtga aggccatgtc
       61 ctggccattg gtcacgtagg tcacacagcc gctggtatga ccgggcgttg ccctcaccgt
      121 cagggagcaa ttgccgaagg caaggacgtc gccgtggtcc acggcgacgt cgacgccttc
      181 gatcccgtaa cgtcgcgaga gcacaatctc ggcatcgagg gaactgtgca tcagccaagc
      241 actggtcaca tgatccgcat ggacgtgggt gtcgagcacg tacctgactt tcaaaccgag
      301 ctctctgatg agcgcgcaat cgcggccgtg ctgctcgaag acggggtcga tgatgaccgc
      361 atcgcgagta tccgcgcacc cgagcaggta ggtatacgtg gacgagtccg catcgaaaag
      421 ctgtctgaat atcatgacgt tccctcaccc taccggtgcg ctcaaagtct accccgcgag
      481 ccgatacctc gcatcctgac gtgtatactc cctttgccgc cgccacggag cgggtgccga
      541 atttcgcggc gtccggactc atcattcgta gccagggccc ttctgctcca gaccccgaaa
      601 ctcaacgtct gccccgggcg attgcctcgc tcccaggcgt tgccgaccgg cacctgaatc
      661 tcgccaagca gctcgagcgt caactcgcgc gcttcactga cgccggtcag gccgatacgg
      721 cagtcgccga atccgcccgg acgctctggc gcgccgcggt gagcgatgtc cagaatggcc
      781 tggacgacga tcgcccgctc tactgggggc gactggcact tcggcgatcc gtctaccgca
      841 gcgccctccg tgaaaaggac tcgctgcttc ggtccatcga gcactactcc cgaggcatgg
      901 gtgaccttcg gctcggcgat gatgtcctca acgtgctgat cacgggattc gacccatttc
      961 acctggagac ccggctcgat cagagcaacc cgtccgggtt ggcagcgctg gcgctgaacg
     1021 gatccgagtt cggttccggc ggtgggcggg ccagagtctg cgcgagcata tttcccgtca
     1081 ggttcgaaga tttcgacgac ggcatcgtcg agcgcttttt ccagcagatc cttgctgagc
     1141 gcgagcccgc catggtggtg acgctgagca tgggccgcga cgccttcgac ctggaacgtt
     1201 tcccgggacg gcggcgctcc gcgcaggatc cggacaatca caacgtgcta accggcgcga
     1261 gcccggacaa acctttgccc ccgggcaaaa ccgggtcgca cctggagggc cccgagttcc
     1321 tggagttctc tctcccggtg catgccatgc gggcggcgga cggccactgg ccggtccgcg
     1381 acaaccgatg ggtcaccacc atcgagcgtg gggagttcga ggcgggatct ctggcggacc
     1441 tgcaatccca gctggccgtg cgaggttctg gagggggtta cctgtccaac gagatcgcgt
     1501 accgaagccg tctgctccat gggcaactcg gctgcagctg ccccatcggc cacgtccaca
     1561 cccccgctat ccagggccac aacgcagcgc tggagcgact catggtggga cagatccggc
     1621 agatgctggc gcaggctgcc gccagcctga cgccccggcc gccgtcctag ctcggatccg
     1681 cgatcttcag cagctgcttg ccgaggttac gaccggtgaa cagcctgttc agggtgtccg
     1741 gaatgttctc gaagccttcc tgaacgtctt cctgatgaac gatctcgccg ctgttgatcc
     1801 aaccgagcag ctcggggacc gcgatgtgcg aacggtccat gtaatcgatg acgatgaagc
     1861 cctccatgcg cgcccgcatg atgaccaggt tcatgaggtt caccggcccc ggctgcggct
     1921 ccgtggcgtt gtacgccgat atgcccccgc agaggacgac gcgcgcgcgc atgttgatgt
     1981 gattcagcgc ggcctcgagg attccgccgc caacgttgtc gaaaaagacg tccacccggt
     2041 tcgggcacaa ctcgccgatc cggcggtcga cgtcttcgtt cttgtaatcg atcacgtcgt
     2101 cgaagcgcgc ctcgtccttg agccacgcgc acttctccgg gccaccggcg atcccgacca
     2161 cccgacatcc tttgatacgc gcgatctggc cggctaccga tcccgtcgcg cccgcggccc
     2221 ccgatacgag caccgtctcc ccggccttcg gctggccaag atccagcatg ccccaatagg
     2281 cggtcagccc ggtcaccccg agcgcgccca gggcaagacg cggtgttacc ccctccggca
     2341 gcttgcgcag ccccatgggc cccatgcccg ccgacatcac cgtgaagtcc tgccagcccc
     2401 ccgtggtctg aacgagatca ccggcgctga agtcgtcgct tctgctctca acgacctgac
     2461 ccacggaccc agcgcgcatc acctcgccga tctggactgg cggcaggtag ctttcacgat
     2521 cctccatcca tccgcgctga gtcggatcga aagagaggta aagattgcgg atgagcactt
     2581 cgccatctcc gggctccgga atggtttctt cccgatactc gaagttgtct ttggtgacca
     2641 ttccatacgg ccgtttggcc agtacccatt gtctgttgac gttcatcagc ggtcctccat
     2701 gcgagcgctt gattaaatca tcggcaaagg tcagagtcac cgcggtctgc gaatctgata
     2761 ggggacaatg agcgacatcg cgttcctctc ggccaccgag ctcgcccgca ggatccgcga
     2821 ccgcgagctt tccagcgaag agctgctgct gcattacttg cagagggtcg accgctacaa
     2881 cggcgccctc aacgccatcg tggtggacat ccgggaggag gcattgatgg cggcgcgcgc
     2941 cgccgatgcg gcggtagccc gaggcgtcga cgtgcccccc tttcacggtg tgcccatgac
     3001 ggttaaggag tcatacaacg tcgccggcac ccccacgacc tggggcaacc ccaaatggag
     3061 gggcaacgtc gcgttcgagg acgccgaatc ggtgaagcgt ctgaaggacg ccggcgtgat
     3121 catcttcggg aagaccaacg ttcctctcgc gctggccgac ttccagagct tcaacgccat
     3181 ctacggcacc accaacaacc cattcgatca ctcgcgcgcg ccgggcggct cgtcgggggg
     3241 gtcggcggcg gcgctggcgg ccgggctcac gggtctcgag gccggctccg acatcggagg
     3301 ttctattcgc aatcccgcac acttctgcgg ggtcttcggt cacaagccga catggaacct
     3361 cctgtggatg cgcggacacg caccgccagg cgatgtgcga gcgagctcag acatctccgt
     3421 catcgggccg ctgggtcgat ctgccgcaga cctggaaacc gcggttcgta ccatggccgg
     3481 gcccgacgac atcatggcgc gggggtacac gctgagcctg ccgaccctga agagaggggg
     3541 gctcggaggg ctcaggatcg ccatatggga ggacgacccc gtgtgtccgg tgagcgccga
     3601 gacgaaggcc cgcgtgctcg ccgtggcgag cgcatgcagg gacgccggtg ccgagatcat
     3661 cgagggcgcg cggcccgact tcagctcaga gcacagccac gagacctacc agaatcttct
     3721 ccagcccacc atggcatcac gcatgccgga cgaggagtac gagagtcttc tggaatatgt
     3781 ggccaatctc gatccgcggg acaccagccg gacggcgcgg gtgcttagag cccaggtcgc
     3841 caggttccgg gattggaaac gcagccacga gctgcgcgag cacctgcgct ggaagtggca
     3901 cctgttcttc caggactacg atctgatgct gaccccgatg atgccgacct cggcgtttcc
     3961 ccacgatcac cgccccatca gccagcgcac catagaagtc gacgggacca gcatgccgta
     4021 tatggatcag atcttctggg ccgggctgac cggggtggcc tacctgccgt ccacggtcat
     4081 accgaccggc ctggacgact cgggcctgcc catcggcgtg cagctggttg ggccggagta
     4141 cggggacctc atcaccatcg gcgtggccag ggagctggag accgccggct tccgcttcgt
     4201 tgcgccaccg gcttacgcct gacgggttgc agccatgggc acaacggctc gcgcgcatcc
     4261 gaacattgcg ctgatcaagt actggggaaa agcgccgggg caggaaaaca tcccggccac
     4321 gccctcactg tccatcaccc tggattccct ggttaccgaa acccacgttg ccaccgggcc
     4381 atcccgggac gagctctact tcgacgacga gccggtggcg gaccgcaagg tgctcgactg
     4441 gctcgaggtg ctgcgtcaac gctacgacat accaccgctg gaaatccgca cggccaacaa
     4501 tttcccaacc gccgccgggc tggcgtcgtc cgcgtccggc ttcgccgcgc tggccgtggc
     4561 tttggatgcc caatgcggcc tgcagctgga tgcggccacg ctctcggatc tcgcccgccg
     4621 cgcgtcggga tccgccgcgc gttcgatatt cggcggctac gtcgccgtgc aggggccgcg
     4681 ctgggtcgcc cagcccgttc tcagcccgga agcctggccc ctgaagacgg tcatcgcggt
     4741 gacgaccacg gcccgaaaat ccgtctcgtc ctccgaggga atggccattt ccgctgccac
     4801 ctcaccctac tacccgggat gggtggagag caccagggcc gacttcaccg acgccctggc
     4861 cgccgtcaga acgcgcgatt ttcaggcgct ggctgagctt gcggagttca gctgcctgaa
     4921 aatgcacggc ctcatgctgg gctcgcggcc cgggcttttg tactggaagg ccgccaccgt
     4981 ctcctgcctc cacgcggttc gcgcgctgag acaggacggc gtggacgtct tctttacggt
     5041 ggatgcgggc ccccaggtca aggcgatctg caaaccgagc gccgagagcg acgtgagagc
     5101 ggccctggca gcggtgccgg gtgtggagca gatcgtcgtc acgggtctgg gttccggcgc
     5161 gcgggtcatc gatccgtgat ccacgcgagc gccccgggta aggtcgtgct ctggggcgag
     5221 tacgcggtgc tcaccggcgc ccccggactg gtgctggccg tcgatcgccg ggccagctgc
     5281 acgctgacgc cggggggcga tgcctggcac ttcgaaaccc ggggactgcc cgagccagcc
     5341 gtggatctcg acgttgccga tctgaccgga gcgcacccgc ccggcccgtc gggcggcgcg
     5401 ctggcatggc acgttctgcg gcatctggac agcgaggtcc cggccggcgc gaccctgacg
     5461 accgacaccg ccagcttcca ggacggcggc cacaagcttg gcatcgggtc gagcgcagcg
     5521 gtttgcaccg ccgtctacgg ggcattctgt gagctgctcg aaatcggccc gagcctgaac
     5581 gacgccctcg ccgtgcaccg aagcctgcag ggtggaagcg gcagcggcat cgacgtcgcc
     5641 gcggcgtatc tcggcgggtc gttgcgctac cagctgcgcg gcggggcccg gcctccggcg
     5701 gcagagccgt tcgatctgcc tgacgggttg atcctgcgct tcatctggac gggaaccccg
     5761 gcacaaacca cccggcacgt cgcgcggttc gaggcatggc gggcggaaaa ccaga
//
LOCUS       DBNHQR010000500         1934 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_111786, whole genome shotgun sequence.
ACCESSION   DBNHQR010000500 DBNHQR010000000
VERSION     DBNHQR010000500.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_111786"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1354)
                     /gene="purB"
                     /locus_tag="AC16VR_11365"
     CDS             complement(<1..1354)
                     /gene="purB"
                     /locus_tag="AC16VR_11365"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018403030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HIR6011798.1"
                     /translation="MQTHPLMTIGALDGRYRNRLEPLAPLVSEFGLMRYRVLVECRWF
                     AHLAQTDSIGELPALSSGQAAALAAVAEDFSLADAERIKAIERTTNHDVKAVEYFVKE
                     RVAAIDGLASHIEFVHFACTSEDVNNLSYALMLRDSRELLGEQMQALIDQISAVADDA
                     AEIPMLSRTHGQAASPTTMGKELKNVAYRLSRQLRQLREVALLGKMNGAVGNYNAHVV
                     AYPEVDWPELARAFVEGLGLAQNPLTTQIEPHDYIAELFHALVRFNQILLDFDRDMWG
                     YIAIEYFVQQPVAGETGSSTMPHKVNPIDFENSEGNLGIANALLEHMAAKLPVSRWQR
                     DLSDSTVLRNVGSACGHCSVAYQASQRGLGRVKINADRIRADLDASWEVLAEAVQTVM
                     RKHGLIEPYEQLKAATRGKRIDRDLYSEMLETLDLPASAREELRALTPASYTGLAADL
                     T"
     gene            complement(1363..1611)
                     /locus_tag="AC16VR_11370"
     CDS             complement(1363..1611)
                     /locus_tag="AC16VR_11370"
                     /inference="COORDINATES: protein motif:HMM:NF016259.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF489 family protein"
                     /protein_id="HIR6011799.1"
                     /translation="MNAPEIDLARRLKGISRLGKRIQVADDPDLLKRESVADTIRAEL
                     LAGVRFAWLWQQLGGRRWHLVLRRRQLLVALETLERKT"
     gene            complement(1608..>1934)
                     /locus_tag="AC16VR_11375"
     CDS             complement(1608..>1934)
                     /locus_tag="AC16VR_11375"
                     /inference="COORDINATES: protein motif:HMM:NF042466.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase beta-barrel
                     domain-containing protein"
                     /protein_id="HIR6011800.1"
                     /translation="PEAPWYVVDKVTDGNTLVVSQSEEDLLSEWLAAGETNWLAPEPE
                     LPRQCTAKVRYRQTDQPCLVSHRADGRLLVRFEQPQRAVTPGQYVCFYDGDVCLGGAL
                     IEATGS"
BASE COUNT          307 a          605 c          665 g          357 t
ORIGIN      
        1 tggtgaggtc ggcggccagg ccggtgtagg aagccggcgt cagcgcgcgc agctcttccc
       61 tggcgctcgc gggcagatcg agggtctcga gcatctcgct gtacagatcg cgatcgatcc
      121 gtttgccgcg ggtggcggct ttcagctgct cgtagggctc gatcaggccg tgcttgcgca
      181 tgacggtctg tacggcctcc gccagaactt cccagctggc gtccaggtcg gcccggatcc
      241 ggtcagcgtt tatcttcacg cggccgaggc cgcgctggct cgcctggtag gcgacgctgc
      301 agtggccgca ggcagaaccg acgttgcgaa gcacggttga atccgacagg tcccgttgcc
      361 agcgcgagac gggcagcttg gcggccatgt gctcgagcag ggcgttggcg atgcccaggt
      421 tgccctcgga gttctcgaag tcgatggggt tgaccttgtg tggcatggtg gacgagccgg
      481 tttcgccggc gaccggctgc tggacgaagt actcgatggc gatgtagccc cacatgtcgc
      541 ggtcgaagtc gagcagtatc tgattgaatc tgaccagcgc gtgaaagagc tcggcgatgt
      601 agtcgtgggg ttcgatctgg gtagtcagcg ggttctgcgc gaggcccagg ccttcaacaa
      661 aagccctcgc cagctcgggc cagtcgactt cgggataggc caccacgtga gcgttgtagt
      721 tgccgaccgc cccgttcatc ttgcccagca gggccacctc acgcagctgc ctgagctgac
      781 gggacaggcg gtaagcgacg tttttgagct ccttgcccat ggtggtgggc gaggccgcct
      841 gaccgtgggt tcgggacagc atcgggatct cggccgcgtc gtcggcgacc gcgctgatct
      901 gatcgatgag cgcctgcatc tgctcgccga gcagctcgcg agagtcgcgc agcatcaggg
      961 cgtaagagag attgttgacg tcctccgacg tgcaggcgaa gtggacgaac tctatgtgcg
     1021 aggcgagccc gtcgatagcc gcgacgcgct ccttgacgaa gtactccacg gccttcacgt
     1081 cgtgattcgt ggtccgctcg atcgccttga tgcgttcggc gtccgcgagc gaaaagtctt
     1141 ccgccacggc ggcaagggca gcggcctggc cggaagatag ggcgggcagc tcgccgattg
     1201 aatcggtctg ggcgagatga gcgaaccagc ggcactccac cagcacgcgg tagcgcatca
     1261 gtccgaactc gcttaccagc ggtgccagtg gttcgaggcg gttccggtag cgcccgtcca
     1321 gggcgccgat ggtcatgagc gggtgtgtct gcacggaggt ggtcacgttt tcctctcgag
     1381 agtttccaag gctaccagca gctgccggcg tcgcaaaacc agatgccacc ggcggccgcc
     1441 cagctgctgc cacagccagg cgaaccggac gccggcaagc agctcggcgc ggatcgtatc
     1501 ggccacgctt tcacgcttga gcagatcggg atcgtcggcg acctggatgc gcttgccgag
     1561 ccggctgatg ccctttagcc ttcgggccag gtcgatctcg ggcgcgttca tgagcccgtc
     1621 gcctcgatca gcgcgccgcc caggcaaacg tcgccgtcgt agaaacacac gtactggccc
     1681 ggggtaactg cccgctgggg ttgctcgaag cgcaccagaa ggcgaccgtc ggctcggtgg
     1741 ctgaccaggc agggttggtc cgtctgacgg tagcggacct tcgccgtgca ttgcctggga
     1801 agctcgggct ccggtgccag ccagttcgtc tcgccggccg ccagccactc gctgagcagg
     1861 tcctcttcgc tctgagacac tacgagcgtg ttgccgtccg tgaccttgtc caccacgtac
     1921 cacggcgctt cggg
//
LOCUS       DBNHQR010000501         2569 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1632104, whole genome shotgun sequence.
ACCESSION   DBNHQR010000501 DBNHQR010000000
VERSION     DBNHQR010000501.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1632104"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="AC16VR_11380"
     CDS             complement(<1..344)
                     /locus_tag="AC16VR_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019689834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HIR6011801.1"
                     /translation="MTYQHIEVPADGEKITVNADDSLNIPDNPIIPFIEGDGIGVDIT
                     PVMKDVVDAAVAKAYGGSRRIHWMEIYSGEKSLEVYGEDEWLPAETLDAMREFIIGIK
                     GPMTTPVGGGIRS"
     gene            complement(470..949)
                     /locus_tag="AC16VR_11385"
     CDS             complement(470..949)
                     /locus_tag="AC16VR_11385"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003316924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030366 - molybdopterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HIR6011802.1"
                     /translation="MHSEVRIQEADFSVAAQWEALRGRASGEVGAVASFVGLVRDRDP
                     DAPIQGLYLEHYPGMTEASIGKILDETASRWPILDIVVIHRVGGLEPGDQIVFVQVAA
                     AHRPAAFAACEFVMDYLKTDAVLWKRERLMEGERWIEATGDDRRRVARWKADPVGDS"
     gene            complement(952..1209)
                     /locus_tag="AC16VR_11390"
     CDS             complement(952..1209)
                     /locus_tag="AC16VR_11390"
                     /inference="COORDINATES: protein motif:HMM:NF014636.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR6011803.1"
                     /translation="MIPVRVLFFASLREAVGREEVFLELPDSARIDDLLASLDDILSA
                     EAMAALRAENVRLAVNQEMLTAPGRLRPNDEVAFLPPVTGG"
     gene            complement(1206..1682)
                     /gene="moaC"
                     /locus_tag="AC16VR_11395"
     CDS             complement(1206..1682)
                     /gene="moaC"
                     /locus_tag="AC16VR_11395"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019821998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HIR6011804.1"
                     /translation="MSKLTHLNAAGEASMVDVSGKSETLRRAVARGIVRMAPETLREI
                     LQSGVPKGDVIAVARVAGIQAAKRCADLIPLCHPLPLTKVALDFSPAGDDGLEVTAEC
                     VVTGQTGVEMEALTAVSVAALTVYDMCKAIDRGMVIESVCLVSKEGGRSGTWERTP"
     gene            1784..>2569
                     /gene="mnmA"
                     /locus_tag="AC16VR_11400"
     CDS             1784..>2569
                     /gene="mnmA"
                     /locus_tag="AC16VR_11400"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HIR6011805.1"
                     /translation="MATPRQTKVIVGMSGGVDSSVAALLLLQEGYAVEGLFMKNWEED
                     DGTEYCTAKEDLADAQRVAERLGITLHLANFAAEYWDNVFEHFLAEYDAGRTPNPDVL
                     CNREIKFKQFADYARALGADMIATGHYVRGAMQDGRFVLLKGVDENKDQSYFLQAVPA
                     EELARCLFPLGALTKTEVRAIAAQAGFENSGKKDSTGICFIGERRFRDFLQRYLPSRP
                     GPIKDTQAMTVGEHLGLAYYTLGQRQGLGIGGLAGRPEAPWYVV"
BASE COUNT          472 a          867 c          796 g          434 t
ORIGIN      
        1 gaccggatgc cgccgcccac cggcgtggtc atggggccct tgatcccgat gatgaactcc
       61 cgcatggcgt cgagggtctc cgccggcagc cactcgtctt ccccgtagac ctccagggat
      121 ttctcgccgg agtagatctc catccagtgg atcctgcggc tgccgccgta ggccttggcc
      181 accgcggcgt ccaccacgtc cttcatgacc ggggtgatgt cgaccccgat gccgtccccc
      241 tcgatgaaag ggatgatcgg gttgtcgggg atgttcagtg aatcatctgc gttgacggtg
      301 atcttctcgc cgtcagcggg cacttcgatg tgctgatagg tcatctaaag ctccgtttgg
      361 aaatccgttt gggtaacagc cttgacccgt cgcgataggt cgcgatggaa aggcgcaccg
      421 gaaaaaggcg cgagattata gcccttccga cgaggctgtg cagtgggtat caggaatcgc
      481 cgaccgggtc ggccttccat cgggcgacac ggcgacggtc gtccccggtc gcctcgatcc
      541 agcgctcgcc ttccatcagc cgctcccgct tccacagcac ggcgtcggtt ttcagatagt
      601 ccatgacgaa ctcgcaggca gcgaaggctg ccgggcggtg cgcggccgcc acctgcacga
      661 acacgatctg gtcgccgggt tcgaggccac ccacgcggtg aatgaccacg atgtccagga
      721 tgggccagcg tgatgcggtc tcgtcgagaa tcttaccgat gctcgcttcc gtcatgccgg
      781 gataatgctc gagatacaga ccctggatgg gcgcatcagg atcgcggtcc ctcacgagcc
      841 cgacaaagct cgctaccgcg ccaacctcgc ccgacgcgcg cccgcgcagc gcctcccact
      901 gtgccgccac gctgaaatcc gcttcctgaa ttcgaacctc ggaatgcacg gtcagccccc
      961 cgttaccggc gggaggaagg cgacctcatc gttgggacga agccgcccag gggccgtcag
     1021 catctcctgg ttgacggcca ggcgaacgtt ttccgcccgc agcgctgcca tcgcctcggc
     1081 gctgagaatg tcatccagcg aggcgagcag gtcgtcgatc cgcgccgaat ccggcagctc
     1141 gaggaagacc tcctcgcgac cgacggcttc cctgagcgaa gcgaaaaaca gaacgcggac
     1201 gggaatcacg gcgtgcgttc ccaggtgccg ctgcggccgc cttctttgct caccaggcac
     1261 acggactcga tgaccatgcc ccggtcgatg gctttgcaca tgtcatagac cgtcagcgca
     1321 gccacgctga cggcggtcag ggcttccatc tcgaccccgg tctggcccgt gaccacgcac
     1381 tcggcggtaa cctcgagacc gtcgtcgccg gccgggctga aatccagagc caccttggtg
     1441 agcggcaacg gatggcaaag cgggatgaga tccgcgcacc gtttggcggc ctgaatgccg
     1501 gctacgcggg cgaccgcaat gacgtcaccc ttgggaaccc ccgactgcag gatctcgcga
     1561 agggtctccg gcgccatccg cacgatcccc cgggccacgg cacgccgcag cgtctcggac
     1621 ttgccggaga cgtcgaccat gctcgcctcg cccgcggcat tgagatgggt aagcttgctc
     1681 atggtcgcat tatagccccc aacgggctgt cgggcgggca agaacccctg cttgggggta
     1741 gaatggccat ccttcacaca gcagtgctgc acgcacccct gaagtggcaa ctcccaggca
     1801 aaccaaagtc atcgtcggca tgtcgggcgg tgtcgactcc tccgtcgccg ccctcctgct
     1861 cctgcaggag gggtacgccg tggagggcct gttcatgaag aactgggaag aggacgacgg
     1921 caccgagtac tgcacggcca aagaggatct ggccgacgcc caacgggtcg ccgaacgcct
     1981 gggcataact ctgcacctgg cgaacttcgc tgccgagtac tgggacaacg tcttcgagca
     2041 ctttctcgcc gagtacgacg cgggccgcac gccgaatccc gacgtgctct gcaaccgcga
     2101 gatcaaattc aagcagttcg cagactacgc ccgcgccctc ggcgcggaca tgatcgccac
     2161 ggggcactac gtgcgtggcg ccatgcagga cggccgcttc gtcctgctca agggcgtgga
     2221 tgaaaacaag gaccagagct acttcttaca ggccgtcccg gctgaggagc tcgccaggtg
     2281 cctctttccc cttggcgcgc tgaccaaaac cgaggtacgt gccattgccg ctcaggcagg
     2341 attcgaaaac tcgggcaaga aggacagcac gggcatctgc ttcatcggtg agcgacgctt
     2401 ccgggacttc ctgcaacgct atctcccaag ccgaccgggc cccatcaagg acacccaggc
     2461 aatgaccgtg ggcgagcacc tgggcctcgc ctactacacc ctgggccagc gtcaggggct
     2521 gggcatcggc ggcctggcag gacgtcccga agcgccgtgg tacgtggtg
//
LOCUS       DBNHQR010000502         3670 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_302648, whole genome shotgun sequence.
ACCESSION   DBNHQR010000502 DBNHQR010000000
VERSION     DBNHQR010000502.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_302648"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..1100
                     /locus_tag="AC16VR_11405"
     CDS             231..1100
                     /locus_tag="AC16VR_11405"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000098 - sulfur amino acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HIR6011806.1"
                     /translation="MAYPRPELLEEPAALAARLDDADLRIVDATVFLVPAERGYRAES
                     GRERYEQGHIPGAAFMDLIDAFSDTTTGLGFSLPDPGVIADALGRLGISDDSEVVVYS
                     TGHLMWATRAWWLLRYVGHQRASVLNGGFDAWRDLGLPFETGTARRAAVTYRPRPDAS
                     LFTDRDGMCRAMDEPGVCTVNALSPEVYAGTGTTNYGRPGHIPGSVNVPYEDLLAGGR
                     FKPAGALAEALESTGLLDADRVVTYCGGGIAATLDGFACLLLGHDNVAVYDGSMSEWA
                     RDPSLPLNVGEAP"
     gene            complement(1177..1824)
                     /locus_tag="AC16VR_11410"
     CDS             complement(1177..1824)
                     /locus_tag="AC16VR_11410"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR6011807.1"
                     /translation="MKNAVVLMRHSAREFAPGRHDLDNPLTEEGRDLARRLGERLPED
                     LTVRAYASPPERCMETAELVLAGYASTGGRVTRHRPVEALGVFYALDQMKMWQVMQSA
                     GGMVPFLEAWFSGDVPQDAVMPPRLAAALVVRLMTDKLSAMDGARRLDVCVSHDMTLH
                     LVRDRLLGQSVRDVDVDFLDGLVAFEREGARWLASHHGEPVKLDAQLLSLGGLWD"
     gene            2006..3238
                     /locus_tag="AC16VR_11415"
     CDS             2006..3238
                     /locus_tag="AC16VR_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008082102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6011808.1"
                     /translation="MDLGISERVKPLIDDITQYIHDYVVPNERAYAEQVDAGGRWTET
                     EAMEEMKEKAKERGLWNFFLPDSERGSGLNNLDYAHVAEVLGRYPLSSEAMNCAAPDT
                     GNMEVIERYGSEQQKEEWLRPLLEGKIRSAFAMTEPQVASSDAANISLEARLEGDEWV
                     LNGEKWWTSGIGDPRCKIMIVMCVTEPDAPRHSRQSQILVPVETDGVEVLKMMHVFGY
                     DDAPHGHGHVRFTDVRVPKDNIILGSGRGFEIAQGRLGPGRIHHCMRSIGVAERALEL
                     MCRRALSKEAFGKPIADLGGNYDKIADARINIEMARLLTLKAAYMMDTVGNKIARSEI
                     AQIKVAVPNIALKVIDDAIQIHGGAGVSQVFPLAKMWAGQRTLRLADGPDEVHRRAVA
                     RLELGKYPDKDPIRTDVW"
     gene            3251..>3670
                     /locus_tag="AC16VR_11420"
     CDS             3251..>3670
                     /locus_tag="AC16VR_11420"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6011809.1"
                     /translation="MGRLDDKVAVITGAGRGIGREIALMMAGEGAKIVVNDLGGNMDG
                     TGTGMIADDVVAEIRAAGGEAVSNTASVAEVAGGESLLQSALGAFGAMDILVNNAGIL
                     RDRTIFKMEEADWDAVQAVHLKGHYCCTRPFARYIREN"
BASE COUNT          712 a         1202 c         1191 g          565 t
ORIGIN      
        1 ggcgctcgcc cagctgcgat cgctccggga acgggcatac gagatgctcg tcgaggagca
       61 gctggccgcg gacaccagct tcctgatctt cgtgctgctg accaacgatg ccatcgccca
      121 gatcgactcc ccggagaacg ggccatccgt ctagcgcggc acgcccgcgg agaggacgag
      181 ccgggatgag gccgtccagc accaaaccga caaaccagca ggagaatcaa atggcatacc
      241 cacgtcctga gctgctagag gaacccgcgg cactcgccgc gcggctggac gatgccgatc
      301 tgcggatcgt cgatgccacc gttttcctcg ttcctgccga gcgcggctac cgagcggaga
      361 gcgggcgcga acgctacgag caggggcaca tacccggcgc cgcgttcatg gacctcatcg
      421 acgcgttctc cgatacgacc acgggcctcg gcttcagcct gccggatccg ggggtcatcg
      481 ccgacgcgct cggccgcctg ggcatctccg acgacagcga ggtggtcgtc tactcgacgg
      541 gccacctcat gtgggctacc cgtgcatggt ggctgctgcg ctacgtcggc catcagcgag
      601 cgtccgtcct caacggcggg ttcgacgcgt ggcgcgatct ggggcttccg ttcgagaccg
      661 gcaccgcgag gcgagcagcg gtgacctacc gcccccgacc agacgccagc ctgttcaccg
      721 atcgggacgg gatgtgccga gcgatggacg agccgggtgt ctgcacggtc aacgcgctct
      781 caccggaggt atacgcgggc accggaacca cgaactacgg ccggccaggc cacatcccgg
      841 gcagcgtgaa cgtgccctac gaagatctgc tggcgggcgg acgcttcaag ccggccggcg
      901 cgcttgccga ggcgctcgaa tccacggggc tgctcgatgc cgaccgggtc gtgacctact
      961 gcggcggggg catcgccgcc acccttgacg gcttcgcctg cctgctcctc gggcacgaca
     1021 acgtggccgt ctacgacgga tccatgtccg agtgggcgcg ggacccctca ttgcccctca
     1081 acgtaggcga ggccccttag gacacatagg gcacataggg cacataggac acttagggca
     1141 cgccatgggc cgggcgccgc gtgcgtccag ctctcctcag tcccacaggc cccctagaga
     1201 gagcagctgc gcatcgagct tcacgggctc gccatgatgg ctcgccagcc agcgcgcgcc
     1261 ctccctttcg aaggcgacca gcccgtccag aaagtccacg tcgacgtcgc gtacgctctg
     1321 gcccagcaac cggtcgcgga cgaggtgaag ggtcatgtcg tgggacacgc agacgtcgag
     1381 ccgtcgcgcc ccgtccatgg ccgaaagctt gtcggtcatc agacgaacca ccagcgctgc
     1441 cgcaagccgg ggcggcatca ccgcatcctg gggaacgtca ccggaaaacc aagcctccag
     1501 gaagggcacc attcctccgg cggattgcat gacctgccac attttcatct ggtccagggc
     1561 atagaagacg ccaagcgcct cgaccgggcg atgccgagtc acccgcccgc cggtggacgc
     1621 gtaaccggcc aggacgagct cggctgtttc catgcatcgc tccggcgggc tggcatacgc
     1681 gcgcaccgtc aggtcctcgg gcaaccgctc cccgagccgt cgggccagat cacgtccttc
     1741 ctcggtgagc gggttgtcca gatcgtgacg cccgggcgcg aactcccgag ccgagtggcg
     1801 catcagcacg acggcgtttt tcacaccgcc cccaaacagg tcgttcagga gccgaacggt
     1861 ggcatcgccg ggcgcggtca atggactccc ccctgtcaaa tagcaccacg taaccttctg
     1921 tattagtatc gaattctcac gccttttaat agagatattc actgtcccgc tcgaacatcg
     1981 ggcaccgggc acccaggggg aagcaatgga cctaggcatc tctgagcgcg tcaagccgct
     2041 catcgacgac attactcagt acattcacga ttacgtggta cccaacgagc gcgcctacgc
     2101 cgagcaggtc gatgcgggcg gtcgctggac cgaaaccgag gccatggagg agatgaagga
     2161 aaaggccaag gagcgcggcc tgtggaactt ttttcttccg gacagcgaac gcggttccgg
     2221 actcaacaac ctcgactacg cgcacgtcgc cgaggtgctg gggcggtacc cgctctcctc
     2281 ggaggccatg aactgcgcgg cgcccgacac cggcaacatg gaagtgatcg agcggtacgg
     2341 ctccgagcag cagaaagagg aatggttacg gccgctgctg gaaggaaaga tccgctcggc
     2401 gttcgcgatg accgagccgc aggtcgcttc ctcggatgcc gccaacatat ccctggaagc
     2461 gaggctcgaa ggggacgagt gggtgctcaa cggcgagaag tggtggacct cggggatcgg
     2521 cgacccccgc tgcaagatca tgatcgtgat gtgcgttacc gagccggacg cgccacgaca
     2581 ctcgcggcag tcgcaaatcc tggtgccggt ggaaaccgac ggcgtcgagg tcctgaagat
     2641 gatgcacgtc ttcggctacg acgacgcgcc ccacggacac gggcacgtcc gattcaccga
     2701 cgtccgagta ccaaaggaca acatcattct cgggtccggt cgcggtttcg agatcgccca
     2761 gggccgactg ggtccggggc gcatccacca ctgcatgcgt tctatcggcg tcgccgagcg
     2821 ggccttagaa ctgatgtgcc gccgcgcgct ctccaaagaa gcgttcggca agcccatcgc
     2881 cgacctaggc ggcaactacg acaagattgc cgatgcgcgc atcaacatcg agatggccag
     2941 gctgctgacg ctgaaggctg cctacatgat ggataccgtg ggcaacaaga tcgccaggag
     3001 cgagatcgcc cagatcaagg tggcggtacc caacatcgcg ctgaaagtca tcgacgacgc
     3061 cattcagatc catggtggcg ccggcgtatc ccaggtattt ccgttggcca agatgtgggc
     3121 aggacagaga actctacgcc ttgccgacgg ccccgacgaa gtgcaccggc gcgcggtggc
     3181 caggctggag ctgggcaaat acccggacaa agatccgatc cgaacggacg tttggtagga
     3241 ggcgatcgtc atgggccgac tggatgacaa ggtggccgtc atcaccgggg cgggtcgcgg
     3301 gatcggccgc gagattgcgc tgatgatggc cggtgaaggt gcgaaaatcg tcgtcaacga
     3361 tctcggcggc aacatggacg ggaccggaac cggaatgatc gccgacgacg tggtggccga
     3421 gattcgcgcg gccggcggcg aggccgtctc gaacaccgca tccgtcgccg aggtggcggg
     3481 cggcgagagc cttctgcagt cggccctggg cgcgtttggc gccatggaca tcctggtcaa
     3541 caacgcgggg atattgaggg accggaccat cttcaagatg gaggaggcgg actgggacgc
     3601 ggttcaagcc gtgcacctga aaggccacta ctgctgcacc cgccccttcg cgcgctacat
     3661 ccgggaaaac
//
LOCUS       DBNHQR010000503         3852 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_745405, whole genome shotgun sequence.
ACCESSION   DBNHQR010000503 DBNHQR010000000
VERSION     DBNHQR010000503.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3852)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3852
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_745405"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="AC16VR_11425"
     CDS             complement(<1..528)
                     /locus_tag="AC16VR_11425"
                     /inference="COORDINATES: protein motif:HMM:NF025637.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIR6011810.1"
                     /translation="MIATLPEFDRAPRVLRLNIAGHPVDWVSWQEAVCLYARDIVIWT
                     LGDPILRIRGGHSRLDSSQSSVEIHAIIACDGRVTRPSRSVPPLTNEALFRRDLNVCL
                     YCGGTFADGALTRDHVVPTSRGGRDVWDNCVAACRRCNHHKGSRLAEECGMELLALPY
                     VPNFAEYLALINSGRI"
     gene            748..1446
                     /locus_tag="AC16VR_11430"
     CDS             748..1446
                     /locus_tag="AC16VR_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011811.1"
                     /translation="MGKVRAMLRLPIMLLFALLVACGRGGILQGGKLEIPQSTGNWFC
                     QPGTGGDEWDCIQDEELARSPQLAAATTPAPPVEESGEGPEAEPPEPAATESPVPRDV
                     EPGAVQEQPPIEEPSAADPEPPKAAEPELDADGLMGLPDDYFAVQLVALDSAAELSAF
                     VDTHGLGYLPHARVERDGRIHHVLLLGFYESYALAEAASLDPPAPIEPGDAWIRPLAG
                     LKRAVARAVSLDAG"
     gene            complement(1452..2348)
                     /gene="rarD"
                     /locus_tag="AC16VR_11435"
     CDS             complement(1452..2348)
                     /gene="rarD"
                     /locus_tag="AC16VR_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HIR6011812.1"
                     /translation="MSEQRAGMLFALAAYGFWGFAPVYFKVLDFAGPVEIIAHRVLWS
                     VPLLALLIGARRQFGALLALRADQVGWLGLSGLLVAVNWGVFVWALLNGRMLETSLGY
                     YINPLVSVLLGVLFLGEWLRRGQLVAVALAAIGVVNEVVSVEVIPWAGLTLAVSFGFY
                     GLVRKRLGVDSAVGLGVEATLLLPVALGYLAYQFEAGQGALDRGEGGEIAWLAASGLV
                     TTFPLVCFAAAALKLPLSTLGFVQYLAPTITFFLAVLVFGEAFRWEQSVTFGCIWLAV
                     VIFSAEGVYHQRRLQRRLGRWA"
     gene            complement(2376..2732)
                     /locus_tag="AC16VR_11440"
     CDS             complement(2376..2732)
                     /locus_tag="AC16VR_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6011813.1"
                     /translation="MKYVRIYSGDDGQSHFEDVEVDLADHGPLGRISDLVRGKGVVFR
                     EVEGEYHYDFHNAPRRQFVVNLTGSVDIEVGDGTVRRMEPGSILLAEDLTGQGHVSRS
                     VNGEPRTCLFIPLADD"
     gene            2839..3732
                     /locus_tag="AC16VR_11445"
     CDS             2839..3732
                     /locus_tag="AC16VR_11445"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR6011814.1"
                     /translation="MDQTSNHVLREIVDILNVERIETNLYRGQNHQTEHVFGGQVLAQ
                     AIAAAYRTVDVPHGLHSIHAYFLRPGDWRVPILYEVDRIRDGRSFSTRRVVAVQNGRA
                     IFSMASSWQKDEEGLEHAVQMPDVPPPGALRGDREAYLEAAREHPEISRFAFRFEAID
                     SRQVERILMTDSGTHPPYKHTWLRTRETLPDDPEIHLAMLAYMSDLDFMSTSMLPHGR
                     NTIREHVQGASLDHSIWFHRPFRADEWLLFAKESPNAGGARGFVRGRFFNRDGELVAS
                     AAQECLIRPRGALAENLASPA"
BASE COUNT          727 a         1347 c         1210 g          568 t
ORIGIN      
        1 gatacggccg ctgttgatca gggccaggta ctcggcaaag ttcggaacgt agggcagcgc
       61 cagcagttcc atcccgcact cctcggccag ccggctgccc ttgtgatgat tgcagcgcct
      121 gcaggccgcc acgcagttgt cccagacatc ccgtccgcca cgggaagtgg gcaccacgtg
      181 atcgcgcgtg agagcgccgt cggcaaacgt cccgccgcag tagaggcaga cgttcaggtc
      241 ccgacggaac agcgcttcgt tggtcagggg cgggacgctc ctggacggcc gcgttacgcg
      301 gccgtcgcag gcgatgatgg cgtgaatttc gacgctggac tgggagctgt ccagccgtga
      361 gtgaccgccg cgaattcgca ggatgggatc gcccagggtc cagatgacga tgtcccgggc
      421 gtacaggcac accgcctcct gccagctcac ccagtccacg ggatggccgg cgatgttcag
      481 tcgcagcacc cgaggcgcgc gatcgaactc ggggagcgtt gcaatcatgc ctggcagctc
      541 aaaacctcag attgatgcgc aatcggggca cgctcgcccc gatggcggcg ccggtatgat
      601 gaccaatcgt tcctgacgat tcaacgtgga gctcccgcgg gcgcccgcgc ctggcccaag
      661 catccagcag cagcgttcgg gccgcttcgt gacccagggg catacggtgc tcgactcgcc
      721 agatcagctt tgacaccaac tgcggcggtg ggcaaagtac gcgccatgct gcgcctaccc
      781 atcatgctgc tcttcgcgtt gctcgtggcc tgcggccgtg gcgggatcct gcagggcggc
      841 aagctggaga tcccgcaatc caccggcaac tggttctgtc agccaggcac cggcggcgac
      901 gagtgggact gcattcagga tgaagagctg gctcggtcgc cacagctggc cgctgcaacg
      961 acccccgccc cccccgtgga ggagtcgggc gagggtcccg aagccgaacc gccagagccc
     1021 gcagccaccg aatcgccggt tccgcgggat gtcgagcccg gcgcggtcca ggagcagcct
     1081 ccgatcgaag agccgtccgc cgcagacccg gagcccccaa aggctgcgga acccgagctt
     1141 gacgccgatg gtctgatggg cctcccggac gactacttcg ccgttcagct ggtcgccctg
     1201 gatagcgcag ccgagctgag cgcgttcgtg gatacccacg gcctcgggta tctgccccac
     1261 gcccgggtgg agagagatgg gcgcatccac cacgtgctgc tgctcggctt ctacgaaagc
     1321 tacgccctcg ccgaggcggc aagcctcgac ccgccagcgc cgatcgagcc cggcgacgcc
     1381 tggattcgtc cgctggccgg cctcaaacgc gccgtggcga gggccgtctc cctggacgca
     1441 ggctgaactg gtcaagccca gcggccaaga cgccgctgca gcctgcgctg gtggtagacg
     1501 ccctcggcgg agaagatcac cacggccaac cagatgcagc cgaacgtgac gctctgctcc
     1561 cagcgaaacg cctcgccgaa cacgaggacg gccagaaaga acgtgatggt gggcgccaga
     1621 tactgaacga agcccagggt cgacagcggc agctttagcg ccgccgccgc aaagcagacc
     1681 agtgggaagg tcgtgacgag cccgctggca gccagccacg cgatctcgcc gccttcgcca
     1741 cgatcgaggg caccctgccc cgcctcgaac tgatacgcca ggtaccccag cgccacgggc
     1801 agaagcaggg tggcctccac gcccagcccc acggccgagt ccactcccag ccgcttacgg
     1861 accagcccgt agaagccgaa gctcaccgcc agggtcaggc ccgcccaggg gatgacctcc
     1921 acggagacga cttcgttcac cacgccgatc gcggcgagag caaccgccac cagctgcccg
     1981 cgacgcagcc actcgccgag aaacagcacc ccgagcagaa cggagaccag cgggttgatg
     2041 tagtacccaa ggctggtttc gagcatgcgc ccgttgagca gagcccagac gaacacgccc
     2101 cagttcacgg ccacgagcag gccgctcaac ccaagccagc caacctgatc cgcacgaagc
     2161 gcgagcagcg cgccgaactg gcgccgcgcg ccgatcagca gcgcgagcag aggaaccgac
     2221 cagaggaccc tgtgcgcgat gatctccacc ggcccggcaa aatccagaac cttgaagtaa
     2281 acgggggcaa agccccagaa gccgtaagcg gccagcgcga acagcattcc cgcgcgttgc
     2341 tcgctcatgt cggtttccgg aatggcagag acgattcagt cgtctgcaag cggtatgaaa
     2401 agacaggtgc gaggctcgcc attgacgcta cggctgacgt gcccctgccc ggtgagatct
     2461 tcggcgagca ggatgctgcc gggctccatg cgccgaacgg tgccatcacc gacttctata
     2521 tccacggacc ccgtcaggtt gaccacgaac tgccggcgcg gagcattgtg aaaatcgtag
     2581 tggtactcgc cttcgacttc gcggaacacc acccccttgc cgcgcaccag atccgagatc
     2641 ctgcccaggg gcccgtgatc ggcgagatcc acctctacgt cctcgaaatg cgattggccg
     2701 tcgtccccgc tgtagatcct cacgtatttc acgtcgctgc ctccgtagtg tccccacccg
     2761 accccgatcc gggcatctca tcgggtgcca tgtccagtag aatgccacca accgtgcggc
     2821 gcagccatcg acccgaccat ggatcagacc agcaaccacg tgcttcgaga gatcgtcgac
     2881 atcctgaacg tcgagcggat cgaaacgaac ctctaccgcg gacagaacca tcagaccgag
     2941 cacgttttcg gcggccaggt gctggctcag gccatcgccg ccgcgtaccg caccgtggac
     3001 gtaccccatg gcctgcactc catccacgcc tatttcctcc gccccggtga ctggcgggtg
     3061 ccgatcctgt acgaggtgga ccggatccgg gacggccgca gcttctccac gcgccgggtg
     3121 gtggccgtcc agaacggacg ggccatcttc agcatggcgt cctcgtggca gaaagacgag
     3181 gaaggactag agcacgccgt gcagatgccc gacgtgccgc cgcccggcgc tctgcgcgga
     3241 gaccgtgagg cgtacctgga agccgcgcgc gagcacccgg aaatcagccg tttcgccttt
     3301 cgtttcgaag ccatcgacag caggcaggtg gagcggatcc tgatgaccga ctcgggaacc
     3361 cacccgccgt acaagcacac ctggctgcgg acccgcgaga ccttgcccga cgatcccgag
     3421 atccatctcg ccatgctcgc ctacatgtcc gacctggact tcatgagcac gtccatgctg
     3481 ccccatgggc gcaacacgat ccgcgagcac gttcagggag cgagcctgga tcactcgatc
     3541 tggtttcacc ggccgttccg ggccgacgag tggctgctgt tcgcaaagga gtcgcccaac
     3601 gcgggcggcg cgagaggttt cgtgcgcggg cggttcttca accgggatgg cgaactcgtg
     3661 gcgagcgccg cacaggagtg cctgatccga ccgcggggcg cgctggccga aaacctcgcg
     3721 agccccgcgt gaccggcaga cctagagcgc ttctagtatc gcctcggcct tgctcacctc
     3781 gaactgcccg ggctcttcga gcgcgagctt ggtgacggtg ccgtcctcca cgatcatcgc
     3841 gtagcgctgg ga
//
LOCUS       DBNHQR010000504         2944 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_492143, whole genome shotgun sequence.
ACCESSION   DBNHQR010000504 DBNHQR010000000
VERSION     DBNHQR010000504.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_492143"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..559
                     /locus_tag="AC16VR_11450"
     CDS             110..559
                     /locus_tag="AC16VR_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6011815.1"
                     /translation="MSENNAVEETKAVLGRRGHVTTSWDELETLQLSTGIETTMFRIG
                     MPDDPSAPTVFKVYYPPGCKVEAHTHECDYSEIVLEGSQKVGNEWLHPGDIRVGMANR
                     GYGPLVAGDEGATVLVIFADGQWPAVTIGRNDGSSLGVDELLKTYGR"
     gene            complement(564..1424)
                     /locus_tag="AC16VR_11455"
     CDS             complement(564..1424)
                     /locus_tag="AC16VR_11455"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6011816.1"
                     /translation="MSGGRLEGKVAIVTAAGRGIGRGIALRLAEAGADLVVNSYGEDT
                     TRSTAEAVEATGRKALQVPGDITDPEKMLELTERALETYGQIDVLVNNVGAGPKSAIE
                     PEPGPLGPVAALWDALYQQNLRPVVLMTEAVLPHLIARKSGRIIHISSIAGRTSLSAP
                     MLTHFVHNSYGAMKAGLINYTQTLAELVGPHNINVNAICPGIVWTDAWRANAERAVTA
                     LPEFAGQDPREWFEGIARGDYPEIFDRTPLRREQTVEDIGNAAVFLASDDAMNITGQS
                     LMVDGGMVKV"
     gene            1614..1970
                     /locus_tag="AC16VR_11460"
     CDS             1614..1970
                     /locus_tag="AC16VR_11460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HIR6011817.1"
                     /translation="MTVIIAGIMELEDPSQVKEMLLAARPHIEGALQEEGCIEYAWTE
                     DHLNPGRVHVYEEWTSSETLEAHLQSHWYRDMGAHLRQYPRKPPTRVIKKYRVDLEEP
                     VYDSEGVARGHFFTES"
     gene            complement(2056..>2944)
                     /locus_tag="AC16VR_11465"
     CDS             complement(2056..>2944)
                     /locus_tag="AC16VR_11465"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIR6011818.1"
                     /translation="DVMVQLTTLPPTATTEEIMEVIHADGAVIVKDMLTGDEVGAVVD
                     EVRPYMDTTPVGADEFAGFKTTRTGALVARSPKCRDLIMNQTVLDATKAFLAPYCLRF
                     QLHLGQIIRLMPGQPAQQIHRDRWAWGKYLKDVEPQFNTIWALSDFTQANGATQVVPG
                     SIDWPDDRVAKPDEICQAVMSAGSALLYTGSVFHGGGENRSDGDRIGMNLTYSLGWLR
                     QEENQYLSCPPEIARELPEEMQNLLGYQLGSYALGYDTPPLPPGEGPEAVGPQWAVGR
                     RDSHEALGDAELLEAVINM"
BASE COUNT          473 a          945 c          992 g          534 t
ORIGIN      
        1 ctcctccccc ttggtgcctg ggattatcac acaccggccg cttcaggcga tcgttgagtg
       61 cccgtgccgg tcttgctaac gttcgggaat tcttgagctg gagcgatcag tgagcgagaa
      121 caacgccgtt gaggaaacca aggccgtgct gggacgccgg ggccacgtca ccacgtcctg
      181 ggacgagctg gaaacgctgc agctttccac ggggatcgaa accacgatgt tcaggatcgg
      241 gatgcctgac gatccgtccg ccccgacggt attcaaggtc tactacccgc cgggctgcaa
      301 ggtcgaggcg cacacccacg agtgtgacta ttccgagatc gtgctggagg gatctcagaa
      361 ggtgggcaac gagtggctgc acccggggga catccgggta ggcatggcaa accggggcta
      421 cggcccgctg gtggccgggg acgagggcgc cacggtgctg gtgatatttg ccgacgggca
      481 gtggccggcg gtcaccatcg gccgcaacga cggcagctcc ctgggagtcg acgagctcct
      541 gaaaacctac ggccgctgag ccctcagacc ttgaccatgc cgccgtccac catcagggac
      601 tggccggtga tgttcatcgc gtcgtccgat gccaggaaga cggcggcgtt gccgatgtcc
      661 tcgaccgtct gctcccggcg cagcggcgtt cggtcgaaga tctccggata gtcgccgcgg
      721 gcgatgccct cgaaccattc ccgcggatcc tggccggcga actcgggcag cgccgtgacg
      781 gcccgctccg cgttggcccg ccaggcgtcc gtccagacga tgccgggaca gatggcgttg
      841 acgttgatgt tgtgtggacc gacgagctcg gccagggtct gggtgtagtt gatgagcccg
      901 gccttcatgg cgccgtagga gttgtgcacg aagtgggtca gcatcggcgc cgacagcgag
      961 gtgcgcccgg cgatggacga gatgtgaatg atcctgccgc tcttccgggc gatgaggtga
     1021 ggtagaaccg cctcggtcat cagcaccacg ggccgaagat tctgctgata gagggcgtcc
     1081 cacagcgcgg ccaccgggcc cagggggccg ggctcgggct cgatggccga cttgggcccg
     1141 gcgcccacgt tgttgaccag cacgtcgatt tgaccgtagg tctccagggc ccgctcggtg
     1201 agctcgagca tcttttccgg atcggtgatg tcccccggga cctgcaacgc cttgcggccg
     1261 gtggcctcca cggcctcggc cgttgatcgc gtggtgtcct caccgtatga gttcaccacc
     1321 agatcggcgc cggcctcggc caggcgcagg gcgatgcctc gcccgattcc cctgccggcg
     1381 gcggtgacga tggcaacttt gccttccagc ctgccaccgc tcatcgcgcg gtccccccgt
     1441 cccgtcccgg cgtctggcgg ctcgactcta tgattgcccg ctgtctgcgt tgaccgttca
     1501 gctcctctcc catcagcctt tgctccctgg tggcggttcc ctcgcatgcc gaggtgattg
     1561 agatagactt tgcatggcaa tttcgggatc agtcaactgc gggagaacga tcgatgacgg
     1621 tgatcattgc ggggatcatg gagctcgagg atccgagcca ggtgaaggag atgctgctgg
     1681 ccgcgcgccc gcacatcgaa ggcgcgctgc aggaggaggg ctgcatcgag tacgcgtgga
     1741 ccgaggacca tctgaacccc gggcgcgtgc acgtctacga ggagtggacg tcctctgaga
     1801 cgctggaggc ccatctgcag agccactggt atcgtgatat gggggctcat ctgcgccagt
     1861 atccgcgcaa gccgcccacc cgggtcatca agaaataccg cgtcgacctg gaagagcccg
     1921 tctacgattc cgaaggcgtc gcgcgcgggc acttcttcac cgaaagctag gatcgggaat
     1981 tgatctggtt ccagacctga tcgggagggc gtgcgcccgt ttttcccagc gcaccgccgt
     2041 cactggcggc cgacgctaca tgttgatgac cgcttccagc agctccgcgt cgcccagggc
     2101 ctcgtggctg tcgcgccgcc ccacggccca ctgcgggcca acggcctcgg gcccttcacc
     2161 gggaggcagc ggcggggtgt cgtaccccag ggcgtagctg ccgagctggt agcccagcag
     2221 gttctgcatc tcctcgggca gttcccgggc gatctcgggt gggcaggaga gatactgatt
     2281 ctcctcctgt cgcagccacc ccagggagta ggtcaggttc atgccgatgc gatccccgtc
     2341 ggagcggttc tcgccgccgc cgtggaagac cgatcccgtg tagagcaggg ccgatccggc
     2401 gctcatgacg gcctgacaga tctcatcggg tttcgccacc cgatcgtccg gccagtcgat
     2461 gctgcccggc accacctggg ttgcgccgtt ggcttgggtg aaatcggaca gcgcccagat
     2521 ggtgttgaac tggggctcca cgtccttgag gtacttgccc caggcccagc ggtcacggtg
     2581 aatctgctgg gccggctgcc cgggcatgag gcggatgatc tgccccaggt gaagctggaa
     2641 ccgcaggcag taaggtgcca ggaaggcctt ggtggcgtcc agcacggtct ggttcatgat
     2701 cagatcccgg catttcgggg atcgggcgac cagcgcgcca gtgcgcgtag tcttgaagcc
     2761 ggcaaactcg tccgctccga ccggggtcgt gtccatgtac ggccgcacct cgtcgaccac
     2821 cgcgccgacc tcgtcgcccg tgagcatgtc cttgacgatc accgcgccgt cggcgtgaat
     2881 gacctccatg atctcctcgg tggtcgccgt ggggggcagg gtggtgagtt gaaccatgac
     2941 gtcg
//
LOCUS       DBNHQR010000505         2482 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1442079, whole genome shotgun sequence.
ACCESSION   DBNHQR010000505 DBNHQR010000000
VERSION     DBNHQR010000505.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1442079"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="AC16VR_11470"
     CDS             <1..528
                     /locus_tag="AC16VR_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017846111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HIR6011819.1"
                     /translation="EIEESKSEYDREKLQERLAKLAGGVAVINVGGATEVAMKERKMR
                     VDDALAATRAAVEEGVVPGGGVALLRASAELAKAAPRNQDQASGVDALRRAAEAPLRQ
                     IVANAGLQPGVVLRKIQSEQGHFGFNAQTETFGDMLEMGILDPCKVVKSALHNAASVA
                     GMMMSTEALVADAPR"
     gene            complement(563..1102)
                     /locus_tag="AC16VR_11475"
     CDS             complement(563..1102)
                     /locus_tag="AC16VR_11475"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6011820.1"
                     /translation="MTSPCKEHPPGDAWVHLREPAARDRGPVASVLVSTGFFSDAEVA
                     VALEVFDAALQGKDSGYEHLVAESAGRVIGYVCWGGPIEMTLSSFDLYWIAVDAAARG
                     RGVGHRLLAAAESLAAASGCAQLFAETSGRADYAPTHSFYESCGYLRAAELADFYAPG
                     DAKVFYVKRLTGGNQAPPE"
     gene            complement(1105..2091)
                     /locus_tag="AC16VR_11480"
     CDS             complement(1105..2091)
                     /locus_tag="AC16VR_11480"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF019121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR6011821.1"
                     /translation="MRVAIAYNKPGDAAGSAEADVLEQVEAVETALVGAGHQVCALPC
                     TLDLAGFDGALAKQRPDVVFNLVEALGGTDRLAVLVTLLLDARGVPYTGAGSRALELS
                     ADKVGAKERMRRLGLPTPDWYVCEKTQVDFPGAGCYIVKARFEHASLGLDDQSVVQVA
                     DAGQLTRRIRARGKHSGAQQFAERYVAGREFNVALLASETGPAQVLPPAEIDFSAFAP
                     GKPRIVGHDAKWSSRAFEYHATPRRFDFPAADARLLDLLGTLAMQVWECFAMRGCARV
                     DFRVDEDGRPWILEINPNPCLSPDAGFAAALDAAGIPAEEMVGRLVRTAATD"
     gene            complement(2082..>2482)
                     /locus_tag="AC16VR_11485"
     CDS             complement(2082..>2482)
                     /locus_tag="AC16VR_11485"
                     /inference="COORDINATES: protein motif:HMM:NF019121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011822.1"
                     /translation="ARVLGTLEIELLATAEANVYSYLNKEECESRVLYRLVDGRADPV
                     VARAEAAALGAWRALGARDGGRIDLRADSAGEPNFIEANPLAGLHPAHSDLPILATAV
                     GMPYAELIRAIVDSASERLAMDATEMNRCA"
BASE COUNT          453 a          829 c          834 g          366 t
ORIGIN      
        1 gaaatcgagg agtccaagtc cgagtacgac cgcgaaaagc tgcaggagcg tcttgccaaa
       61 ctggcaggcg gcgtggcggt gatcaacgtc ggcggcgcca cggaagtcgc catgaaggag
      121 aggaagatgc gcgtggacga cgcgctcgct gctactcgcg ctgccgtgga agagggcgtc
      181 gtgccgggag gcggcgtggc actgctgcgc gcctcagccg agctcgcaaa ggccgcgccc
      241 cgaaaccagg accaggcaag cggcgtggac gcgcttcggc gggctgccga ggcgcccctg
      301 cgccagatcg tcgccaacgc cggtctgcag ccaggcgtgg tgctgagaaa gattcagtcg
      361 gagcagggac acttcgggtt caacgcgcag accgaaacgt tcggcgacat gctggaaatg
      421 ggcatactgg acccctgcaa ggtcgtgaag tcggcgctgc acaacgcagc gtccgtggcc
      481 ggcatgatga tgtcgacgga ggcgctggtc gcagacgcgc cccgctagag agggccgcgc
      541 tcggaacacg gtcggaacgc cgtcattccg gtggggcctg attgccgccg gtcagccgtt
      601 tgacgtagaa aactttggcg tcacctggcg cgtagaagtc cgcgagctcg gccgcgcgca
      661 ggtagccgca ggattcgtag aagctgtgcg tgggcgcgta atcggcacgt ccggaggtct
      721 ccgcaaaaag ctgggcacag cccgacgccg ccgccaggct ctcggccgcg gccagcagcc
      781 gatgaccgac cccgcggccc cgggccgccg catccacggc gatccagtaa aggtcgaagc
      841 tcgagagcgt catctctatg ggcccgcccc agcagacgta cccgatcacc cttccggcgg
      901 attcggccac gagatgctcg tagccggagt ccttaccctg caatgccgca tcgaagactt
      961 ccaacgccac agcgacttcg gcatcactga aaaatcccgt cgagaccagc accgaggcca
     1021 cgggtccacg gtcgcgtgcc gccggctccc gcagatgaac ccaggcgtcc ccggggggat
     1081 gctctttgca gggtgaggtc atgctcagtc cgtagcggcc gtcctgacca gtcgcccgac
     1141 catctcctcc gccgggatcc cggccgcatc gagcgcggca gcgaagccgg catcgggcga
     1201 gagacacgga ttcgggttga tctccagaat ccagggtcgg ccgtcctcgt cgacccggaa
     1261 gtcgacgcgg gcgcagccgc gcatcgcgaa gcactcccag acctgcatcg cgagggttcc
     1321 aagcaggtcg agcaaccggg catcggctgc ggggaagtcg aaacgccgcg gggtcgcatg
     1381 atactcgaag gcgcggctcg accacttggc gtcatgcccg acgatgcggg gtttgcccgg
     1441 cgcaaacgcc gagaagtcga tctccgccgg aggcagcacc tgggcggggc cggtttccga
     1501 agccagaagc gccacgttga actcacgccc ggcaacgtag cgttcggcga actgctgcgc
     1561 gccactgtgc tttccgcgtg cgcggatgcg cctggtgagc tgcccggcat cagcaacctg
     1621 aacgaccgac tggtcgtcca ggccaaggga ggcgtgctca aagcgtgcct tgacgatgta
     1681 gcaccctgcc ccagggaaat ccacctgggt cttctcacag acgtaccagt ccggcgtcgg
     1741 caggccgagc ctgcgcattc gttccttggc gccgacctta tcggcgctga gctccagcgc
     1801 ccgcgatccc gctccggtgt aggggacacc gcgggcgtcg aggagcagcg tcaccagcac
     1861 ggccaggcgg tcggttccgc ccagggcctc gaccagattg aagacgacgt caggccgctg
     1921 ctttgccagg gcaccatcga atccggcgag atccagcgtg cagggcaacg cgcagacctg
     1981 gtgaccggcg cccacgaggg cagtctcaac cgcttcgacc tgctcgagca cgtcggcttc
     2041 cgcgctgccc gcagcgtctc ccggcttgtt gtaagcgatg gctacgcgca acggttcatt
     2101 tccgtagcgt ccatggcaag acgttccgaa gccgagtcga cgatggcacg aatgagctct
     2161 gcataaggca ttcccaccgc ggtagccaga atgggcagat cggaatgagc gggatgcagc
     2221 cctgccaggg ggtttgcctc gatgaaattg ggttcccccg ccgagtccgc gcgaaggtcg
     2281 atgcgccctc cgtcccgggc cccgagggcc cgccaggcgc cgagcgccgc ggcctcggcc
     2341 cgagcaacga cgggatcggc acgaccatcg acgagtcggt acagcacacg ggactcgcac
     2401 tcctccttgt tgaggtacga gtagacgttc gcctcggcag ttgccaggag ctcgatctcg
     2461 agagtcccga gcaccctggc at
//
LOCUS       DBNHQR010000506         6158 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_556191, whole genome shotgun sequence.
ACCESSION   DBNHQR010000506 DBNHQR010000000
VERSION     DBNHQR010000506.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6158)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6158)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6158
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_556191"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..1086)
                     /gene="cysD"
                     /locus_tag="AC16VR_11490"
     CDS             complement(121..1086)
                     /gene="cysD"
                     /locus_tag="AC16VR_11490"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018403501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HIR6011823.1"
                     /translation="MAYRAAESVARHNDSPPRRHPGQELSHLDRLEAESIHIMREVAA
                     QAENPVMLYSVGKDSSVMLHLARKAFYPSVPPFPLMHVDTRWKFKAMYDFRDQMARET
                     GMELIVYINPEGVEKDINPFDHGSALHTDVMKTQGLKQALDLHGFDVAFGGARRDEEK
                     SRAKERVFSFRTSSHRWDPKNQRPELWNLYNGYKSPGESIRVFPLSNWTELDIWEYIY
                     RERIPIVPLYFAAERPVVERDGMLIMVDDDRMRLFPGEKIQTRRVRFRTLGCYPLTGA
                     IDSTASDLSAIVLELLQSRTSERQGRAIDTDSSGSMEKKKQEGYF"
     gene            complement(1745..2578)
                     /locus_tag="AC16VR_11495"
     CDS             complement(1745..2578)
                     /locus_tag="AC16VR_11495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR6011824.1"
                     /translation="MRIGVSLPVRELENDLGAIRAFAQLAEHLGLTHLRVPDQVLRQG
                     NKHLHEPLTLLAWIAGATERIELVPSVIVLPSRQTALVAKQAAEIDVLSNGRLRLGIG
                     VGGSPDEYAAMGMDYTTRGARCEEQMKLLRLLWTRKIVSFDGRWDKVNEAGLNPLPVQ
                     RPIPLWIGARGVPAQRILHRIGRLADGWFVLCTPEELPALDGAIAGAAREAGRDPGEI
                     GTEAGVAVVGPREAEWQDRVVGWRQAGLTHLCLRTLGGGLSPAEHMSKLEEVVDRLRE
                     L"
     gene            complement(2661..3872)
                     /locus_tag="AC16VR_11500"
     CDS             complement(2661..3872)
                     /locus_tag="AC16VR_11500"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6011825.1"
                     /translation="MGLLFQAVVFGIGFFCFTFWVAPWRAEFGSSRGEVMAILVTVQI
                     VMGCAAPFAGRAMDHLSIRALVVAGAVCFAAALALAAHATALWQIGLIYSVLFVAGAL
                     LAGPLAAQTLAAKWFFARRGTAIGLSSVGTSLGGFVLPLLVTWLIAAYGWRQANLVLA
                     ALVVVVVVPVVWLVVSNTPGDRGVAPEPEASRPDGTSPVAFPQWTTRTILSERAFWVA
                     VIAFAPMMAAFGAAQQNFAPYTADLGIDARATSYLVSLMAIVMVAGKVFFGAMSDRLD
                     HRLLYWIAVGVLIVSLVLMTRTPAYGLLVVISGLLGFAAGGFLPLLGAVVGSRFGPAA
                     FGRVMGLLGPFTTVAAIGPWIAGNLRDASGTYDDAWMLFVGIMVPAALAMAFMTPRPQ
                     PLALASAERAS"
     gene            complement(3960..4715)
                     /locus_tag="AC16VR_11505"
     CDS             complement(3960..4715)
                     /locus_tag="AC16VR_11505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6011826.1"
                     /translation="MRLGALLGPIVDASEPNALADQARRYAGEGFESLWSAQAIGRGA
                     MYTDPFVALSVAAAVTEKVELGTAVVQVPLYHPVDLAHRALSLRQVAGERVLLGVGAG
                     STQTDFDAFGRDYASRFRTFGESVEVLRQLFEEGRAGETNLSPWPAVLGGLPLLLGSW
                     GKGVERAAASFDGWIASAHYRTPDQVVEALDGYRAAGGRRAVVSTILLGAGTDLGETG
                     DLLGRFAEAGFDDAVVMLLPGGPSATDVRRLVA"
     gene            complement(4746..5966)
                     /locus_tag="AC16VR_11510"
     CDS             complement(4746..5966)
                     /locus_tag="AC16VR_11510"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6011827.1"
                     /translation="MNEPSGPLADITIIDCTMALAGPFGAAILADLGANVIKVEPPDG
                     DVARSVPPLPEDYANANSDQSAGCDFGGYFASVNRNKRSVVIDLKTPAGRETLLALCD
                     KADAIIENMRTGVMDRLGLGYEVLRARNPALVYGCIRGFGDPRTGRSPYAEWPAYDIV
                     AQSMGGIAHITGPESSHGFPAGASIGDLYPGTLLALGVVAAVHHARRTGEGQFMDVGM
                     YDAVMFLSETVIANFGYAGTELGPRGAHHPNLCPFGIFPTSDGAVAIAAPGPGHWQAL
                     CQAIGRPDLVDDEKTRNTFVRRKHQHFVEGVVSDWTATRTRAEVVAALGGRVPCGPVN
                     TAADIFADPHVAARGMVSQVELPGENGEVSIVGTPIKFTGTPTGLYRRPPMLGEHTEE
                     VLREMGLKGAANDE"
BASE COUNT         1099 a         2134 c         1967 g          958 t
ORIGIN      
        1 tcttgccgtc gtcgacgctg ccgcaggtga tgaagcggag cagatcgagg cgcgcctggt
       61 ttgcgatgta ggcgtcgacc cgctcctgca ggtcttcctg aataacttct gcttcggcca
      121 tcagaaatag ccctcctgtt tcttcttctc catggacccg gaggagtccg tatcgatggc
      181 ccggccctgg cgctcactgg ttcggctctg caggagctcg agcacgatgg cggacaggtc
      241 gcttgccgtg gaatcgatgg cgcccgtgag cgggtagcag ccgagggttc gaaagcgcac
      301 ccgccgggtc tgaatcttct cgccggggaa caaccgcatg cggtcgtcgt ccaccatgat
      361 cagcatgccg tcgcgctcga cgacggggcg ctcggccgcg aagtagaggg gaacgatggg
      421 gatgcgctca cggtagatgt attcccagat gtcgagctcg gtccagttcg acaggggaaa
      481 cacccggata ctctcgcccg ggcttttgta gccgttgtac aggttccaca gctcagggcg
      541 ctggttcttc ggatcccagc ggtggctcga cgtgcggaac gagaacacgc gctccttcgc
      601 ccggctcttc tcctcgtccc ggcgggcgcc gccaaaggcg acgtcgaagc cgtggagatc
      661 cagcgcctgc ttgagaccct gggttttcat cacgtcggtg tgcagagcgc tgccgtgatc
      721 gaaggggttg atgtctttct ccaccccttc ggggttgatg tagacgatca gctccatgcc
      781 cgtctcccgg gccatctggt cgcggaagtc gtacatggcc ttaaacttcc agcgcgtgtc
      841 cacgtgcatg agcggaaacg gcggtacgct gggatagaag gccttacggg cgaggtgcag
      901 catcaccgag ctgtccttgc ccaccgagta cagcatcacc gggttctccg cctgggcggc
      961 gacttcccgc atgatgtgga tgctttccgc ctccagccgg tccaggtgtg aaagctcctg
     1021 ccccgggtgc cgacggggcg gcgagtcatt atggcgagcg acgctttcgg cggcacgata
     1081 ggccagcgtc cccatccgcg ccttgccgcc ggaaggcagc tcgatatcgt gggagtctgc
     1141 ccgttccacc agcgcccgac aggcgcgcgc tacctcccca tggggccaga tcagcagctg
     1201 cttgggcgct tcccgaccgc cggcaagcac gtccagcgcg ttcaggagcc catcgacatc
     1261 cagccgcccg ccatccaggt agagccagcg tctgcccatg cgcccgtcgt tggcgaagag
     1321 catgagcccc gccccgggaa cctcgtgagc ctggagaatc agggagagat gacgcttacg
     1381 cttggcttcg gcttccagag catccaccac cagccggacc ggctcgcggc cgagctcgtc
     1441 gcgcacctcg tccagcttga gcgtggcggg cagcaggtcg gcgctttcca gaagacgctc
     1501 cgtcgacctg agagactgag cgctgcccga ttcgctgagc agctcgtgga cggcggccgt
     1561 atcccaggca ccctgggcct gggaccggtt cttgtaaagg ctggcgggta gctccactgg
     1621 gcgcgcacct cgaatgtcgt gatgcgcaat aatattcgaa atcagcgcgc ctgcaacgaa
     1681 ttagacacgg attaatgtaa ccaatacgta caaatctgca tattgcgaca cccaacagcg
     1741 ggcctcaaag ctcccgcaac cgatcgacca cttcttccag cttgctcatg tgctctgccg
     1801 gcgacagccc gccgcccagg gtccgcaggc agaggtgcgt cagcccggcc tgccgccacc
     1861 ccacaacccg gtcctgccac tcggcctccc gcggtcctac cacagccaca cccgcctccg
     1921 ttccgatctc cccaggatca cgtcccgctt cccgtgccgc cccggcgatg gcgccgtcga
     1981 gcgcgggaag ctcctcgggc gtgcacagca cgaaccagcc atcggcaagc cggccgatgc
     2041 ggtgaagaat ccgctgcgct ggaaccccgc gggcgccgat ccacagggga atcgggcgct
     2101 gcacgggcaa agggttcaag cccgcctcgt tgaccttgtc ccagcgcccg tcgaagctca
     2161 cgatcttccg ggtccacagc agtcgcagca gcttcatctg ctcctcgcag cgggcgccac
     2221 gcgtcgtgta gtccatcccc atggccgcgt actcgtccgg gctgccgccg acgccgatgc
     2281 ccagccgcaa ccggccgtta gagagcacgt cgatctccgc ggcctgcttg gcaaccaaag
     2341 ccgtctgccg cgacggcagg acgatgaccg acggcacgag ctcgatgcgt tcggtcgcgc
     2401 cggctatcca ggcgagaagc gtcaggggct cgtgcagatg cttgttaccc tgacgcagca
     2461 cctggtcggg tacccggaga tgggtcagcc cgaggtgctc ggccagctgc gcgaacgccc
     2521 tgatagcacc cagatcgttt tcaagctccc gcacgggtag cgatacgccg attcgcatgt
     2581 ggatcctccc ttagagttct ccctcgcgac gccgagaggg tcagttttcg agatgagtca
     2641 gcctgttgtg tccatcactt tcacgacgcc cgttcagcgc ttgcaagagc gagcggctga
     2701 gggcgcgggg tcatgaacgc catggccaga gccgccggaa ccatgatgcc gacgaagagc
     2761 atccaggcgt catcgtaggt tcccgaggcg tcgcgcagat tcccggcgat ccagggaccg
     2821 atggcagcaa cggtggtgaa cggcccaagc aggcccatga cccgaccgaa cgcggccggc
     2881 ccgaagcggc tgcccaccac cgcgccgagc agcggcagga aaccgcccgc cgcgaatccc
     2941 agcaggccac tgatcaccac cagcagcccg tacgccgggg tccgggtcat gagcaccagc
     3001 gagacgatga gcacacccac ggcgatccag tagagcaggc gatgatccag gcgatcggac
     3061 atggcgccga agaacacctt gccggccacc atcacgatgg ccatgagcga gacaaggtat
     3121 gacgtggctc gggcgtcgat gccgagatcg gccgtgtagg gcgcgaaatt ctgctgggcg
     3181 gcgccaaatg ccgccatcat cggcgcgaac gcgatgacgg ccacccagaa ggcccgttcc
     3241 gagagaatgg tgcgcgtcgt ccattggggg aacgccaccg ggctggtgcc gtcgggccga
     3301 gatgcctccg gttccggggc aacgccgcga tcgcccggcg tgttactcac caccagccag
     3361 accacgggca ccaccacgac caccaccagg gcagccagga ccaggttggc ctggcgccac
     3421 ccgtaggcgg ctatgagcca ggtcaccagc agcgggagca cgaagccgcc cagggaggtc
     3481 cccaccgacg acaggccgat ggccgtgccc cgacgggcga agaaccactt ggccgccagg
     3541 gtctgagccg ccagcggtcc cgccagcagc gcgccggcaa cgaacagaac cgaatagatg
     3601 aggccgatct gccacagcgc ggtggcgtga gcggcaagcg ccagggccgc agcgaaacag
     3661 accgcgcccg caaccaccag agcccggatc gacaggtggt ccatggcgcg gcccgcgaag
     3721 ggtgccgcgc agcccatcac gatctgaacg gtgaccagga tggccatgac ctcgccccgg
     3781 ctgctgccga actccgcgcg ccacggcgcc acccagaagg tgaagcagaa aaagccgatg
     3841 ccgaagacca cggcctggaa caaaagcccc acgcccagca cgttccagcc gtagtaccag
     3901 cggggttcgg ttcgtgctcg cggcgtgccg tcgatgttgc gggctccccg gggcctgggt
     3961 caggcgacca ggcgccggac gtccgtggcg gatggcccgc cgggcaggag catgaccacg
     4021 gcgtcatcga atccagcctc cgcaaagcgg ccgagcaggt cgccggtttc gcccaggtcg
     4081 gtgccggctc cgagcaggat ggtggacacc acggcgcgcc ttccaccggc ggcgcggtag
     4141 ccatccaacg cctccaccac ctggtccggg gtgcggtagt gggcagaagc gatccagccg
     4201 tcaaatgacg cggccgctcg ctcgacgccc ttgccccacg agcccagcag cagcggcagc
     4261 ccgccgagca cggccggcca gggagagaga ttggtctccc ccgcacgccc ttcctcgaag
     4321 agctgcctga gcacctcgac cgactcgcca aacgtccgga agcgggaagc gtagtcccgg
     4381 ccgaaggcgt cgaagtccgt ctgggtcgac cccgccccca ccccgagcag cacacgctcg
     4441 ccggctacct gccgcagcga aagcgcgcgg tgagccagat cgaccgggtg gtaaaggggc
     4501 acctgaacca ccgcagtgcc gagctctacc ttttcggtca ccgccgccgc cacggaaagc
     4561 gcgacgaagg ggtccgtgta catggcgcca cgcccgatgg cctgggccga ccagagactt
     4621 tcgaaaccct cgccagcgta tcggcgcgcc tgatccgcca gcgcattggg ctcggacgcg
     4681 tcgacgattg gtcccagcaa tgccccaagc ctcatgacaa ctcctccttc attcgaattg
     4741 atcgctcact catcgttggc ggcacctttt aagcccattt ctcgaagcac ctcttccgta
     4801 tgctcgccca gcatgggcgg tcggcggtag aggccggtgg gggtacccgt gaacttgatg
     4861 ggggtgccca cgatggaaac ttccccgttt tctcccggca gttccacctg gctgaccatg
     4921 ccgcgcgccg ccacgtgggg gtcggcgaag atgtccgcag cggtgttgac cggcccgcac
     4981 ggcaccctgc ccccgagcgc ggccaccacc tcggccctgg tgcgcgtcgc cgtccagtct
     5041 gacacaacgc cctccacgaa atgctggtgt ttgcgccgca cgaacgtgtt gcgcgtcttc
     5101 tcgtcgtcca cgaggtcggg ccggccgatg gcctgacaga gcgcctgcca gtgtcccgga
     5161 ccaggagcgg cgatggccac cgcgccgtcc gaggtcggga aaatgccgaa cgggcagagg
     5221 ttgggatgat gcgcgccccg ggggccaagc tccgtgccgg cgtagccgaa gttggctatc
     5281 accgtctcgg agagaaacat cacggcatcg tacatcccca cgtccatgaa ctgcccttcg
     5341 cccgtccgtc tggcatggtg gacggccgcg accactccca gggcgagcag agtccccgga
     5401 tagagatcgc ctatgctggc gccagccgga aacccgtgag agctctcagg ccccgtgatg
     5461 tgcgcgatgc cgcccatgct ctgcgccacg atgtcgtagg cgggccactc ggcgtatgga
     5521 ctgcggcccg tgcgcggatc cccgaaaccc cggatgcagc catagaccag ggccgggttg
     5581 cgcgcccgca gcacctcgta gccgagcccg aggcgatcca tcaccccggt tcgcatgttc
     5641 tcgatgatgg catccgcctt gtcgcacaac gccaggaggg tctcgcgccc cgccggggtc
     5701 ttcagatcaa tgaccacact gcgcttgttg cggttgacgc tggcgaagta gccgccgaaa
     5761 tcgcagccgg ccgactggtc cgagtttgcg ttggcgtaat cctcgggcag cggcggcacg
     5821 cttcgggcca catccccgtc cggcggctcc accttgatga cgttggcgcc gaggtcggca
     5881 agaatcgccg caccaaacgg ccccgccagc gccatggtgc aatcgatgat ggtgatatcg
     5941 gccagcggcc cagagggctc gttcatgcgc catctctcct cccggagcaa ctatacccgc
     6001 accgatcggg ccggcacacc tcgaagcgcc cgtggccggg cgaccccgct tttagctaga
     6061 attttcgatc tcgacaaaca ggcgaaaccg ccgggggaag aacatgggca tgttggatgg
     6121 caaggtagcg ctggtcaccg gagccggtgg agggcttg
//
LOCUS       DBNHQR010000507         3320 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1442312, whole genome shotgun sequence.
ACCESSION   DBNHQR010000507 DBNHQR010000000
VERSION     DBNHQR010000507.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1442312"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="AC16VR_11515"
     CDS             complement(<1..233)
                     /locus_tag="AC16VR_11515"
                     /inference="COORDINATES: protein motif:HMM:NF018649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HIR6011828.1"
                     /translation="MGLFELYGVAALTVWVMVTTLWAVSVPTKDASIIDMFWGPLFVA
                     IAWVLLPVYGGGVQAKPYLVMLLVTLWGLRLAY"
     gene            complement(331..984)
                     /locus_tag="AC16VR_11520"
     CDS             complement(331..984)
                     /locus_tag="AC16VR_11520"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007152156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6011829.1"
                     /translation="MTDLLDCVEIDPGGTAGSVIWLHGLGADAHDFEPVVPLLPLNRP
                     LRFVFPNAPVRPVTINAGMEMRAWYDIDPGNPLSGDSDIQSSAKAIAGLVEREHDRGV
                     PYDGITLAGFSQGGVIALELGLSFPHRLAGIMALSTYVPDHEHLVDRIGFANVDAPIF
                     MAHGIGDPMIPITRAITSREALLAMNYEVEWHEYGMGHQVCPEEIADIGTWLNTIYR"
     gene            1118..2947
                     /locus_tag="AC16VR_11525"
     CDS             1118..2947
                     /locus_tag="AC16VR_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009686258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HIR6011830.1"
                     /translation="MEDGLDRQASRELTRLLPALGFLRPYVRQVVFASIALVVTAGVT
                     LSIGQGMRLVIDEGLGSGSAAVLVQSISIFAVLMVVLTAGTFLRFYFVSWVGERVSAD
                     IRQAVFAHLIRLHPGFFETNLPTEIQSRITTDTTVLQTVIGSSVSIALRNVLMFVGGL
                     VLLFVTNPKLSLIVVASVPFVVAPVILFGRRVRALSRDSQDTLAEVGSFAGESLRNIK
                     IVQSFNHEGLDIEAFRQRVERAFQVAVRRILQRSWLVALVMMLVLGAIATMLWVGGRD
                     VLAGQTSPGELAAFIFYAFIVAGSVGAISEVYSDLQRAAGAMERLVELLNSPSLLTDP
                     ESPVLPAAREPSVAVAPGGRLTVRNLGFAYPSRPDAPVLRGLSFAVAPGEMVALVGPS
                     GVGKSTLFDLLQRFYDPDAGQILLDGVDLRELRLADVRERVGLVPQDPVLFAGTVESN
                     LIYGAPDAGDDEKSRALELAHASAFIEALPEGLATVVGEGGVGLSGGQKQRLAIARAL
                     LKRPEILLLDEATSALDAESEQHIRASITELKGQCSIVVIAHRLSTVREADRILVLSE
                     GRLQASGRHEELLKSSELYARFSRIQFAGEDEADGSGARTTAG"
     gene            3015..>3320
                     /locus_tag="AC16VR_11530"
     CDS             3015..>3320
                     /locus_tag="AC16VR_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007977274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HIR6011831.1"
                     /translation="MGPLHGFRIVELAGIGPGPFCGMMLSDMGAEVIRVDRISGGANR
                     PKDVLARNRRSIAVDLKSPAGAETVLRIVEKSDALFEGFRPGVTERLGLGPEDCWARN
                     "
BASE COUNT          528 a         1059 c         1109 g          624 t
ORIGIN      
        1 tgataggcca gccgaaggcc ccacagggtg acgagcagca tgaccaggta aggcttggcc
       61 tgcacaccgc caccgtagac gggcagcagc acccaggcta tggccacgaa cagcggcccc
      121 cagaacatgt cgatgatgct tgcgtccttg gtcggcacgc tgacggccca cagtgtcgtg
      181 accatcaccc atacggtcag tgcggcgacc ccgtacagct cgaacagtcc catgtctatc
      241 tccactctat ctccgctcga tcctcgcggc cgttactgaa ttcgcgggtg cgccttaggg
      301 ccacccgagt tctcgctctt tcccgacgca tcagcggtag atggtgttca gccacgtgcc
      361 gatgtcggcg atctcctccg ggcacacctg gtggcccatg ccgtactcgt gccactcgac
      421 ctcatagttc atcgccagca gcgcctcccg cgaggtgatc gcacgggtga tggggatcat
      481 cggatctcca atgccgtgtg ccatgaagat gggggcgtcc acgttggcga acccgatccg
      541 atccaccagg tgctcgtgat cgggcacgta ggtggagagt gccatgatcc cggccagcct
      601 gtgggggaac gacaggccca gctccagcgc gatgacgccc ccctgggaaa accccgcgag
      661 cgtgatgcca tcgtatggca cgccgcggtc gtgctcgcgc tctaccagcc ccgcgatcgc
      721 cttggccgag gattgaatgt ccgaatcgcc ggacagcggg ttcccgggat cgatgtcgta
      781 ccaggcgcgc atttccatgc cggcattgat ggtgaccggg cgaacgggcg cgttggggaa
      841 cacgaagcgc agcggccggt tcagcggcag caggggcacc actggttcga agtcgtgcgc
      901 gtcggcgccc agcccgtgca gccatatcac cgaaccggcg gttccgcccg ggtcgatctc
      961 gacgcagtcc agcagatcag tcaccgtatt tcccgacggg cgagctccgc ggcagctaaa
     1021 caagatctcg gggggaaagc aatctccgtt tctctcgcca gggtcggtca acgcggcggc
     1081 cctacgttcg cggccctgca ggtgagatac tacggccatg gaggacggac tcgacagaca
     1141 ggcatcacgg gagctgacgc ggctgctgcc cgctctgggc tttctgcgcc cgtatgttcg
     1201 ccaggtggta ttcgcgagca ttgccctcgt cgtgacggcg ggcgtgacgc tttccatagg
     1261 ccaggggatg cgcctggtca tcgacgaagg cctgggcagc ggatcggccg ccgtgctcgt
     1321 gcagagcata tcgattttcg ccgttctcat ggtggttctc acggccggga cgtttctgcg
     1381 tttctacttc gtctcctggg tgggagagcg cgtcagcgcg gacatccgcc aggcggtctt
     1441 cgctcatctc atccgccttc atccaggttt cttcgagacg aatctgccca ccgagatcca
     1501 gtcgcggatc accaccgaca cgaccgtgct gcagaccgtc atcggatcgt ccgtgtccat
     1561 cgccctgcgc aatgtcctga tgttcgtcgg cgggctggtg ctcctgttcg tgaccaatcc
     1621 gaagctctcg ctgatcgtgg tggccagcgt gcccttcgtg gtggccccgg tgatcctgtt
     1681 cggccggcgc gtgcgggcgc tgtcgcgcga cagccaggac acccttgccg aggtgggaag
     1741 ctttgccggc gagtcgctgc gcaacatcaa gatcgttcag tccttcaatc acgaagggct
     1801 ggacatcgaa gcgttccggc agcgggtgga gcgggctttt caggttgccg tccgccgtat
     1861 cctgcagcgc tcgtggctgg tcgccctggt catgatgctg gtgctcgggg ccatcgcgac
     1921 catgctctgg gtgggggggc gggacgtgct cgccggccag acctcgcccg gagagcttgc
     1981 cgccttcatc ttctatgcct tcatcgtcgc aggctcggtc ggtgccatca gcgaggtcta
     2041 cagcgacctg cagcgagctg ccggtgccat ggagcgcctc gtggagctgc tgaactcgcc
     2101 atcgctcctg accgatcccg agtcgcccgt cctgccggct gcccgggagc cgtcggtggc
     2161 ggttgctccg ggtggccgcc tgacggtccg gaacttgggg tttgcatatc cgagccggcc
     2221 ggatgcgccc gtgctgcgcg gtctgagctt tgctgtggcc ccgggagaga tggtcgccct
     2281 ggtggggccg tcgggcgtgg gaaagagcac gctgttcgat ctgctgcagc gtttttacga
     2341 tccggacgcc gggcagattc tgctcgacgg cgtcgacctg agggagttac ggcttgccga
     2401 cgttcgtgaa cgggtgggtt tggtgcccca ggatcctgtg ctctttgctg gtaccgtaga
     2461 gagcaacctg atctacggcg cgcccgacgc gggcgatgac gaaaaatccc gcgcgctcga
     2521 gctcgcccat gccagcgcct tcatcgaggc tctgcccgag ggcctggcca cggtggtggg
     2581 cgagggtggc gtcgggctct ccggagggca gaagcagcgg ctggccatcg cccgcgccct
     2641 tctgaaacga ccggagatcc tgctgctcga tgaggcgacc agcgcgcttg atgccgagag
     2701 cgagcagcac atccgggcga gcatcaccga actcaagggc caatgcagca tcgtcgtcat
     2761 cgcccaccgt ctgtccacgg tccgcgaggc ggatcggata ctcgtgctga gcgagggacg
     2821 gctccaggcc agcgggcgcc acgaggagct tctcaaaagc agtgagctgt atgcccgttt
     2881 ctcgcgtatt cagttcgccg gcgaggacga agcggacggc tcgggtgcta gaaccaccgc
     2941 gggatgatgc ccacgcaaaa gatcgctaaa ctcctcggct gatagtctga tttcttaccg
     3001 gggggaaggt gagcatgggg ccgttacacg ggtttcgtat tgttgagctg gctgggatcg
     3061 gtcccggacc tttctgcggg atgatgctgt ccgacatggg cgccgaggtg atccgggtgg
     3121 accggatcag cggcggggcc aaccgcccga aggacgtgct tgcccgcaat cgacgctcca
     3181 tcgccgtgga cctgaagagc ccggcgggcg cggaaaccgt gctgcgcatc gtcgagaaat
     3241 cggatgcgct gttcgagggt ttccgcccgg gcgtgaccga gcggctcggc cttggccccg
     3301 aggattgctg ggcccgcaac
//
LOCUS       DBNHQR010000508         4405 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1188715, whole genome shotgun sequence.
ACCESSION   DBNHQR010000508 DBNHQR010000000
VERSION     DBNHQR010000508.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4405)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1188715"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..76)
                     /locus_tag="AC16VR_11535"
     tRNA            complement(1..76)
                     /locus_tag="AC16VR_11535"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Lys,seq:ttt)
     gene            complement(177..590)
                     /locus_tag="AC16VR_11540"
     CDS             complement(177..590)
                     /locus_tag="AC16VR_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011832.1"
                     /translation="MIPNPSKALVDLAMKLSTGVLPEIGSPYAAANAGMTAVLLIMLS
                     QDSERAIANRMNDIDELKALFADAAAFPDRVGLASFVDAEPDSLLLADVDRLHADGMR
                     LLIDLHAWAESHDAGLDQEIWAFLGRHADRNRFDV"
     gene            complement(587..1630)
                     /locus_tag="AC16VR_11545"
     CDS             complement(587..1630)
                     /locus_tag="AC16VR_11545"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HIR6011833.1"
                     /translation="MSAVLDTLGERFAAYLTKSWKQPATTLHIEQIPGGASRETYRVR
                     VSIGGQEQGLILRRDPVSSLIDTERSLEYRTYEAVFPTSIPVPEPLLLEEDAVHLERP
                     FSVMREIGGCESSAQSLAAPPYQAMRETIGRRKWEILGQLASLDLSELGVDAFMAVPD
                     DPARRELEHWSKVIADDALHPQPIAEAARRWLERNLPEPSGRFALVHGDYRSGNFLYD
                     EEGVIRGVLDWEMAHIGDPLEDLAWSLDPLWSWPERELAGSLLPREQAIGIWEQASGI
                     TVDRDAFRWWQVFASYKGLAIWISSTEDFVNGASKEPILAVAGWLMSDRQNRILVDRL
                     NPRSDHAYAEPIV"
     gene            complement(1650..2990)
                     /locus_tag="AC16VR_11550"
     CDS             complement(1650..2990)
                     /locus_tag="AC16VR_11550"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6011834.1"
                     /translation="MAEAYEFHDDDVIAPEVYGRHGHPHGAFAWLRANDPLRYVEPTE
                     YLPFWAVTKHADIVEIEKQPEIFASEPRPILVKEAQSPEVRQDVLMQIFERLKDSPKL
                     LEVLATIEQGGLIRSLVQMDPPDHPKYRTLVQPWFKPSNIKRLEDSLTRIIANILDEM
                     MGDGSERELEFVQDVAVWPPLKLITELLGVPEEDEWRILKLTNELFAGDDPEMKRAGD
                     DPLSIIDTIKDLYDYFTRLTDERRANPTEDLSSYIANGRIDGEYLPYKELISYYIIVA
                     TAGHETTRTAISGGLLALLENPEQMSLLRNNIDDEDLIKLAVEEMIRWTTPVSQFSRT
                     AMRDYELRGKQIRQGDALGLFYASANRDEEIFDDPFNFRVDRQPNRHLAFGTGPHICL
                     GILLARLEMRIFFKQFLPRIEKMELVDRGPEYLKASFVHGLKHLPIRFQLRPAR"
     gene            3135..3911
                     /locus_tag="AC16VR_11555"
     CDS             3135..3911
                     /locus_tag="AC16VR_11555"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6011835.1"
                     /translation="MKLSKLSRSVEGKVVIVTGAASGMGRATARVFADEGAKVAAMDI
                     NAEPLNEVVDEIAAAGRDIKGWALDLSDPDAIVEVFAEVPDHFGGIDILVNNAGISMF
                     APIDSDDYEHAWERSLTVLLTAHTRTIRAALPYLRKAEAARIINIASTEGLGATKFGS
                     PYTAAKTGVIGLTRSLAVELGEEGITVNCICPGPILTGMTAAIPDEAKAEFARRRVAL
                     KRYADPEEVAHATLSLALPAACFITGVALPVDGGLTIRNA"
     gene            3929..>4405
                     /locus_tag="AC16VR_11560"
     CDS             3929..>4405
                     /locus_tag="AC16VR_11560"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6011836.1"
                     /translation="MADGSPTPYADPRARVPNTAVVAYGAPGIGAGYMYLMMSLYVMK
                     FSTDVLLIAPAVMGVIFSVSRIWDAVSDPLAGYLSDRTRSRMGRRRTWILGSMIPIGG
                     AFIMVFAPPDGLSDGALVAWMAVAIIGFYSAMTGFLVPHLSLGAELSESYHERSRLF"
BASE COUNT          749 a         1363 c         1470 g          823 t
ORIGIN      
        1 tggtgggtcg tctggggctc gaacccagga ccaacgggtt aaaagcccgg tgctctacca
       61 actgagctaa cgaccctctt gccggatggc ggcaaaaaag agcgcgaatt gtagtcagcg
      121 taagggccca tctcaaccct tgccggtggg gtcgcttcca tgggagcggc gcccgttcag
      181 acgtcgaagc ggttccgatc ggcgtggcgt cccaggaatg cccagatctc ctgatcgagc
      241 ccggcgtcgt gggattccgc ccaggcgtgc aggtcgatga ggagcctcat accgtctgcg
      301 tgcagccggt ccacgtctgc cagcaggagc gagtcgggct cggcatcgac gaaggacgcg
      361 agcccgaccc gatccggaaa cgccgcggcg tccgcgaaaa gagctttgag ctcgtcgatg
      421 tcgttcatgc ggttcgcgat ggcccgttcg ctgtcctgtg agagcatgat cagcaggacg
      481 gccgtcatgc ccgcattcgc tgccgcgtaa ggtgagccga tttccggaag cacgccggtg
      541 gaaagcttca tggccaggtc gacgagagcc ttcgaggggt tgggaatcat acgatgggct
      601 ccgcgtaggc gtggtcggag cgggggttca agcggtccac cagaatccgg ttctgacggt
      661 cgctcatcag ccagccggcc actgccagta tgggctcctt ggacgcgccg ttgacgaagt
      721 cctcggtgga ggagatccag atcgccagcc ccttgtagga ggcgaatacc tgccaccagc
      781 ggaaggcgtc tctgtccacg gtgatgccgc tggcttgttc ccaaataccg atggcctgct
      841 cccgcggcag cagggagccg gcaagctcgc gctcgggcca tgaccacagg ggatcgaggg
      901 accatgccag atcctccagg ggatcgccga tgtgggccat ctcccagtcc agcacgcccc
      961 gaatgacgcc ttcttcatcg tagaggaagt tgcccgagcg atagtcgccg tggaccaggg
     1021 caaagcggcc cgacggctcg ggcagatttc gctccagcca gcggcgggca gcttccgcga
     1081 tgggctgggg atgcagcgcg tcgtcggcaa tgaccttcga ccagtgctcg agctcccgtc
     1141 gtgcaggatc gtccggcacc gccatgaagg cgtcgacgcc cagctcgctt aagtcgagcg
     1201 acgcgagctg gccgagaatt tcccactttc gccggccgat ggtctcgcgc atggcctggt
     1261 aaggcggtgc tgcaagggac tgcgcgctgg actcgcagcc gccgatctcg cgcatcacgg
     1321 aaaacggccg ttccaggtgg acggcgtcct cctcgagcag cagcggctca ggcacgggga
     1381 tcgacgtcgg aaatactgcc tcgtaggttc ggtactcgag ggaacgctcg gtgtcgatca
     1441 ggctggaaac gggatcgcgc cgaagtatga gtccttgctc ttgcccaccg attgaaaccc
     1501 tgacgcggta ggtttccctg gaagcgccgc cgggaatctg ctcgatgtgc agcgtcgttg
     1561 ccggctgctt ccagctcttt gtcaggtaag cggcaaagcg ctcgcccagg gtatcgagaa
     1621 ccgcgctcat gacccggttg cccgtgtgct caccgcgccg gtctcagctg gaaccgaatc
     1681 ggcaggtgct tgagcccgtg cacgaagctt gccttgaggt actccggacc ccggtcgacc
     1741 agttccatct tctcgatgcg gggcaggaac tgcttgaaga atatccgcat ctccagccgc
     1801 gccagcagga tgccgagaca gatgtgaggc ccggtcccga aggcgaggtg acggttgggc
     1861 tggcggtcga ccctgaaatt gaaggggtcg tcgaagattt cctcgtcccg gttggcggag
     1921 gcgtagaaca ggcccagcgc gtcgccctgg cgaatctgct tgccgcgcag ctcgtagtcc
     1981 cgcatggcgg tgcgggagaa ctgactgacc ggcgtcgtcc agcggatcat ctcctccaca
     2041 gcgagcttga tcaggtcctc gtcgtcgatg ttgttccgca gcagactcat ctgctccggg
     2101 ttttcgagca gggcgagcag accaccggaa atggccgtgc gcgtggtctc gtggccggcc
     2161 gtcgcgacga tgatgtagta ggagatcagc tccttgtacg gcaggtactc gccgtcgatg
     2221 cggccgttgg cgatgtacga ggaaaggtcc tccgtggggt tggcgcgccg ttcgtcagtc
     2281 aagcgggtga agtagtcgta caggtccttg atggtgtcga tgatggagag cggatcgtcg
     2341 cctgcccgtt tcatctccgg gtcgtcgccg gcaaacagct cgttggtgag cttgaggatc
     2401 cgccactcgt cctcctcggg cacgcccagc agctcggtga tcagcttgag cggcggccag
     2461 acggcaacgt cctggacgaa ctccagctcg cgctcggagc cgtcgcccat catctcgtcg
     2521 agtatgtttg cgatgatgcg ggtcaagctg tcctcgaggc gtttgatgtt ggatggcttg
     2581 aaccagggtt ggaccagcgt ccggtacttc gggtggtctg ggggatccat ctggaccagc
     2641 gagcggatca acccgccctg ctcgatggtg gccagcacct ccaggagctt cggcgagtcc
     2701 ttgagcctct cgaagatctg catcaggaca tcctggcgca cctccgggct ctgtgcctcc
     2761 ttgaccagga tcggccttgg ttcgcttgcg aagatctccg gctgcttctc gatctcgacg
     2821 atgtcggcgt gcttggtcac ggcccagaag ggaaggtact cggtgggctc gacgtagcgc
     2881 agcgggtcgt tggcgcggag ccaggcaaac gcgccatggg gatggccgtg gcgcccgtaa
     2941 acctccggag cgatcacgtc gtcgtcgtgg aattcgtagg cttctgccat cttcgaggga
     3001 actcccgccg gtgggtgaag tggtttacgt tagcgggatt ccccggccga ttgcaatgtg
     3061 cgatggtgct ccctagagtg gcacgggacc gcatcgaacg ggcagaccgc tccggccgtc
     3121 acgaggtaca cgatatgaag ctcagcaagc tcagccgatc ggtggaaggc aaggtggtca
     3181 tcgtcaccgg tgcggccagc ggtatggggc gcgccacggc gcgggtcttt gccgacgagg
     3241 gcgccaaggt cgcggccatg gacatcaacg ctgagccgct caacgaggtt gtcgacgaga
     3301 tcgctgccgc gggccgtgac atcaagggct gggcgctcga tctttccgac cccgacgcca
     3361 ttgtcgaggt gttcgcagag gtgcccgatc atttcggcgg catcgacatt ctggtgaaca
     3421 acgcgggcat atcaatgttc gcgcccatcg acagcgatga ttacgagcac gcctgggaac
     3481 gatccttgac cgtgctcctc accgcgcata ctcgaacgat ccgcgcggcg ctcccttatc
     3541 tgcgcaaggc cgaggctgcc aggatcatca acatcgcttc caccgagggg ctcggcgcga
     3601 cgaagttcgg cagcccttac acggcggcga agacgggcgt catcgggttg acgcgctccc
     3661 tggcggtgga gcttggtgag gaaggcatca ccgtgaactg tatctgtccg ggaccgatcc
     3721 tcacgggcat gacggctgcc attcccgacg aagccaaggc cgagttcgcg cgacgccggg
     3781 tggccctcaa gcgctatgcg gatcccgaag aggtcgccca cgccacgttg agcctggcgt
     3841 tgccggcagc ctgcttcatc acgggcgtag ccctgccggt ggatggcggt ctgaccatcc
     3901 gcaacgcctg agccgcgggc ggggtcgggt ggcagacggc tcgccgacgc cgtacgcgga
     3961 cccccgcgct cgcgtgccca acaccgccgt ggtcgcctac ggagcgcccg gtatcggggc
     4021 tgggtacatg tacctgatga tgagccttta cgtcatgaag ttctccacgg acgtgctgct
     4081 catcgcgccg gccgtcatgg gcgtcatctt cagcgtatcg cgtatctggg acgcggtatc
     4141 cgatccgctg gccggctacc tcagcgatcg cacgcgcagc cgcatgggcc gaaggcgaac
     4201 ctggattctg ggcagcatga ttcccatcgg cggtgccttc atcatggtct tcgcgccgcc
     4261 ggacggcctc agcgacggcg ccctggtggc ctggatggcc gttgccatca tcggattcta
     4321 ctccgcaatg accggattcc tggtgccgca cctttcgctg ggtgccgagt tgtccgagag
     4381 ctaccacgag cggagccgac tgttc
//
LOCUS       DBNHQR010000509         4089 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1633120, whole genome shotgun sequence.
ACCESSION   DBNHQR010000509 DBNHQR010000000
VERSION     DBNHQR010000509.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4089)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1633120"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="AC16VR_11565"
     CDS             complement(<1..725)
                     /locus_tag="AC16VR_11565"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6011837.1"
                     /translation="MSLITARGLAKSFGDVRALDGLDLTVEAGRIVGLIGPNGSGKTT
                     ALKAILGLSHLDAGTLDVLGMNPATSRKALMQRTAYIADVGILPRWMRVSQLLDFVAG
                     VHPGFRREVAEAHLESTDVRPDKRINGLSKGMNVQLHLALVLAIDAELLVLDEPTLGL
                     DIIYRQKFYDALLGDYFDGNRSILVTTHEVREIEHILTDVVFIHHGRSLLSLSMESIH
                     DEFAKVVAQPTQAGAVRSLGPIA"
     gene            complement(722..1087)
                     /locus_tag="AC16VR_11570"
     CDS             complement(722..1087)
                     /locus_tag="AC16VR_11570"
                     /inference="COORDINATES: protein motif:HMM:NF012610.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR6011838.1"
                     /translation="MPDWKDNEHIYLQLKDLVLRQIMLGTLLEGEPVPSVRQVASQER
                     INPLTVSRAYQMLVDEGLLEKRRGVGMFVCEGAREQALKQERTRFLEEDWPEVVERIA
                     LLGLTRDELLNTAAEEGRQ"
     gene            1287..2519
                     /locus_tag="AC16VR_11575"
     CDS             1287..2519
                     /locus_tag="AC16VR_11575"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6011839.1"
                     /translation="MARGPTTVSTTFQAGWRSPLMLLMLMSVAMPVAFNAWSALLNNF
                     VVERAAFTGVEIGILQSLREVPGFLAFTTIFVLLVVREQTFAVLSLAMLGLGVALTGF
                     LPSEYGLYFTTVVMSTGFHYFETLKQSLSLQWLHRDEAPGMLGRLLAVGSLTSLVTYG
                     VLWVLLELFRLDYVWNFLISGGVCVGLAVVMWLWFPHFETKTPQTKRLVLRRRYWLYY
                     ALTFFSGARRQIFVVFAAFLMVEKFGYSAAQVTLLFLINYAFNWAFAERIGRLIGRFG
                     ERAALTFEYIGLIGVFVGYAFVSDPYVAAGLYVVDHMFFALAIAMSTYFQKVADPADI
                     ASSAGVSFSINHIAAVVVPAALGLLWIHSNALVFLIGAGFAVCSLLLSQLIPERPAPG
                     NEIARGRLFPQPADSAGR"
     gene            complement(2542..>4089)
                     /locus_tag="AC16VR_11580"
     CDS             complement(2542..>4089)
                     /locus_tag="AC16VR_11580"
                     /inference="COORDINATES: protein motif:HMM:NF012363.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HIR6011840.1"
                     /translation="TAAGPLCAQRAGVISGDGFTPTRVAAGSEDCLYLDIYAPANARR
                     LPVMFWIHGGGNSVGHNGPYDGARLATWRDVVVVTVNYRLAHLGWFAHPALTTGNRLH
                     DSGNYGTLDLIQALTWVRDNIQAFGGDPDNVTVFGESAGGTNTLSMLASPRAAGLFHR
                     AIAQSGGLTPMPMARASAYAEDGGHPSSAREIVNRLLIADGRASSPEDARRVQENMPA
                     GAIRDYLYGKSLTELFAVFDNGFAGMIDTPEALGDGHVLPDGPLADVFGDPSRHNAVP
                     VILGSNRDEPALFMAASPEHVNTYLWIFRRLKDEAGYRRQVKYLGQAWKARGVDEFAN
                     AMAAAGNPHVYAYRFDWDEEPSVLGFDLSVALGAAHALEIPFVFGSFSGGLGLGYLYH
                     ESEGRHALAESITSYWTEFAYNGDPGTGRDGGEVRWLPWGRNGATSIVLDTLEDKGIY
                     MTTERVTTDSVKTAIATDPDVPDQRERCLLYYRAFRWGSQFDVDEFAGFGDGGCAAYD
                     PAALGDF"
BASE COUNT          592 a         1337 c         1383 g          777 t
ORIGIN      
        1 tccgcgatgg gcccgaggga cctcaccgcg ccggcttgcg tcggttgagc gaccaccttg
       61 gcgaactcgt cgtggatgct ttccatcgag aggctcaaca ggctcctgcc gtggtgaatg
      121 aacaccacgt ccgtgaggat gtgctcgatc tcgcggacct cgtgggtggt gaccagaatg
      181 ctgcgattgc cgtcgaagta gtcgcccagc agcgcgtcgt agaatttctg ccggtagatg
      241 atgtcgagcc ccagggtcgg ctcgtccagc acgagcagct cggcgtcgat ggccagcacc
      301 agggccaggt gcagctggac gttcatgccc ttggacagcc cgtttattcg tttgtccgga
      361 cggacgtcgg tggattcgag atgggcttcc gctacctcgc ggcggaagcc gggatgcacg
      421 ccagctacga agtccagcag ctgggagacg cgcatccagc ggggcaatat gcccacgtcg
      481 gcgatgtagg ccgttcgctg catcagcgct ttccggctgg tcgccgggtt catgccgagt
      541 acgtcgaggg tgccggcgtc gaggtgagac aggccgagga tggctttcaa ggcggtggtc
      601 ttgccggagc cgttggggcc gatcaggccg acgatgcgcc cggcctccac ggtgaggtcg
      661 aggccgtcca gggcgcgcac gtcgccgaag gatttggcaa gaccgcgggc ggtgatgagg
      721 ctcattggcg gccctcctcg gcagcggtgt tcaggagctc gtcccgggtc aggcccagca
      781 gggcgatgcg ctcgaccacc tcgggccaat cctcttcgag aaaacgcgtt ctctcctgtt
      841 tcagggcctg ctccctggca ccctcgcaga cgaacatgcc cacgccgcgg cgtttttcta
      901 aaaggccctc gtccaccagc atctggtagg cgcgggagac ggttagggga ttgatgcgct
      961 cctggcttgc gacctggcgc accgagggga ccggctcgcc ttcgagcagc gtgcccagca
     1021 tgatctgccg caggaccagg tccttcagct gaagatagat gtgctcgttg tccttccagt
     1081 cgggcacggg gccgtcgctc tatatgtgca gtagtgttat acataaccat aacaccataa
     1141 cgattccggg ggcaaccccg gcttattcgg gcccctgcgg ccatcagggc ccggtttctg
     1201 ggcccgttca tggcgtcgag ttccggcggc gcctccttta gaatcgcccc ttcgcccagc
     1261 cgggcatatc actggaccgc accgcattgg cccgaggacc cacaaccgtc tccaccacgt
     1321 tccaggcagg atggcgcagc ccgctgatgc tgctgatgct gatgtcggtg gccatgcctg
     1381 tcgcgttcaa cgcctggtct gccctgctca acaacttcgt ggtggagcgt gcggccttca
     1441 ccggcgtgga gatcggcatt ctgcagagcc tgcgcgaggt gccgggtttc ctggcgttca
     1501 ccaccatctt tgtgctcctc gtggtgcgcg agcagacgtt tgccgtgctg tcactggcca
     1561 tgctggggct cggcgtcgcg cttaccggct ttctgccttc ggagtacggt ctttatttca
     1621 ccaccgtggt catgtccacc ggctttcatt acttcgagac gctcaagcag tccctttccc
     1681 tgcagtggct gcaccgggac gaggcgcccg gcatgctggg acggctgctt gccgtggggt
     1741 cgctcacctc gctggtgacc tacggcgtgc tatgggtgtt gctcgagctg ttccggctgg
     1801 actacgtctg gaactttctg atctcgggtg gcgtctgcgt ggggctggcc gtggtcatgt
     1861 ggctgtggtt tccccacttc gagacgaaaa cgccccagac gaagcgcctg gtgctgcggc
     1921 gccgctactg gctctactac gcgctgacgt tcttctccgg cgcccggcgg cagatcttcg
     1981 tggtcttcgc ggcgtttctg atggtcgaga agttcggcta ctcggcggcc caggtgacgc
     2041 tgctgttcct gatcaactac gccttcaact gggcgttcgc cgagcgcatc ggccggctca
     2101 tcggccgctt cggcgagcgg gcggcgctca ccttcgagta catcggcctc atcggcgtgt
     2161 tcgtgggcta cgccttcgtg tcggacccgt acgtggcagc cggtctgtac gtggtggacc
     2221 acatgttctt cgcgctggcc atcgccatga gcacctactt ccagaaggtg gccgatccgg
     2281 cggatattgc cagctccgcc ggggtgtcct tctccatcaa ccacatcgcc gcggtggtgg
     2341 tgccggccgc tctgggcctg ctctggatcc actccaacgc cctggtcttt ctcatcggcg
     2401 ccggtttcgc cgtctgctcg ctgctgctct cccagctgat ccccgagcgc ccggcccccg
     2461 gcaacgagat cgcccggggc cggctgtttc cgcagccggc cgattccgcc ggccgctgac
     2521 gccgcctccc ctggagatcg gtcagaagtc gcccagggca gcggggtcgt aggcggcgca
     2581 gccgccgtcg ccgaatccgg cgaactcatc gacgtcgaac tgcgatcccc atcggaaagc
     2641 ccggtagtag agcaggcagc gctcgcgctg atcggggacg tccgggtccg tcgcgatggc
     2701 ggttttcacc gagtctgtgg tcacgcgctc ggtggtcatg tagatgccct tatcctcgag
     2761 ggtgtccagc acgatggacg tggcgccgtt ccggccccag ggcaaccagc ggacctcacc
     2821 gccgtcccgt cccgtgcccg ggtcgccgtt gtaggcgaac tccgtccagt aggaagtgat
     2881 gctctcggca agcgcgtggc ggccttcgct ttcgtggtag aggtagccga gccccagccc
     2941 gcccgagaag gaaccgaaga cgaaggggat ctccagggcg tgggccgcgc cgagggcgac
     3001 gctcagatcg aagcccaaca cgcttggctc ctcgtcccag tcgaaacggt aggcgtagac
     3061 gtgggggttg ccggcggccg ccatggcgtt ggcgaactca tcgacgccgc gtgccttcca
     3121 cgcctggccg agatatttca cctggcgccg gtagccggcc tcgtccttca gcctgcggaa
     3181 gatccacaga taggtattca cgtgctcagg gctcgcggcc atgaacaggg cgggctcgtc
     3241 ccggttgctg ccgaggatca cgggcacggc gttgtggcgc gaggggtcgc cgaacacgtc
     3301 ggccagcgga ccatcgggaa gcacgtggcc gtcgccgagg gcctccggcg tgtcgatcat
     3361 cccggcgaac ccgttatcga acacggcgaa cagctcggta aggctcttgc cgtaaagata
     3421 gtcgcggatg gcgccggccg gcatgttctc ctgaacccgc ctggcgtcct ccggggagct
     3481 tgcgcggccg tcggcgatca gcaggcggtt gacgatctca cgagcgctgc tggggtggcc
     3541 gccgtcctcg gcgtatgcgc ttgcccgggc catgggcatg ggcgtgagcc cgccgctctg
     3601 ggcgatggcc cggtgaaaca ggccggccgc ccggggcgag gccagcatgc tgagcgtgtt
     3661 ggtgccgccg gcggactcgc cgaagacggt gacgttgtcc gggtcgccgc cgaacgcctg
     3721 gatgttgtcg cggacccagg tcagcgcctg gatgaggtcc agggtgccgt agttgcccga
     3781 gtcgtgcagc cggttgccgg tggtcagggc ggggtgggcg aaccagccga ggtgggcgag
     3841 ccggtaattc accgtgacca ccaccacgtc gcgccaggtg gccagacggg cgccgtcgta
     3901 ggggccgttg tgccccacgg agttgccgcc gccgtgtatc cagaacatca ccggcaggcg
     3961 ccgggcgttg gccggggcgt agatgtccag atacagacag tcctcgctcc ccgccgccac
     4021 ccgggtgggc gtgaacccgt cgccggagat cacccccgct ctctgcgcgc acagcggtcc
     4081 ggcagcggt
//
LOCUS       DBNHQR010000510         5204 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1378166, whole genome shotgun sequence.
ACCESSION   DBNHQR010000510 DBNHQR010000000
VERSION     DBNHQR010000510.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5204)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5204)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5204
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1378166"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1745)
                     /locus_tag="AC16VR_11585"
     CDS             complement(<1..1745)
                     /locus_tag="AC16VR_11585"
                     /inference="COORDINATES: protein motif:HMM:NF023282.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MtrB/PioB family outer membrane beta-barrel
                     protein"
                     /protein_id="HIR6011841.1"
                     /translation="MTARLLIVFTLLIAGAASTRSAAAATFTGHVSVGYREVDVSGDE
                     GKYQQHVNLDDGARLFDLGMVFQPDRDANSPAGMPDRVELNASGLGGDPYESVRVRVQ
                     RYGSYRLTYDHRSSDYFYEDLLLAPEEASVEQSTGGDFHHFDFERNRDALALDLDLSS
                     RAKLNLGFARYEKRGESTTVLDVEREEFELDKPIDESLDRYELGFTYAWDHVTVALGQ
                     TWQEYDNDSQIFLPGFSPGSEADAPTSLDLFFLDQPYGFTSSDQRLSVQVRPSSQWIV
                     ELDARAGNLDLDMTATERAQGTDYLGRPFDREDAGAADVDRDTLLLDASVAYLVNDRL
                     QLNAGVRRYDLEQDGAVLFGDTAGASAWDIETTGFELGVDMAVTTTVTLSGGWSTERR
                     KSVVSHAIEGAGHTEAVDTDRDGFFGRLSYRPSRRAQVTLSFEDDRIDDPYTLASATD
                     ARRYKARASYRWDNGLKVSASYRRTDYENDNTGWISESRQSDIRLTHAGDRLTVSVGA
                     AFVELQREVDQLVTGGSRQDLFAISYGADADFWDGAVRWHVTDRVDLTASYRAYENEG
                     SFRVDRDDVRAGDEIL"
     gene            complement(1753..2601)
                     /locus_tag="AC16VR_11590"
     CDS             complement(1753..2601)
                     /locus_tag="AC16VR_11590"
                     /inference="COORDINATES: protein motif:HMM:NF025890.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR6011842.1"
                     /translation="MRLFSMAVGTLFLLATGQVGGNPTCAACHGELVADFHGTPHGAR
                     TEDDEASGCRSCHGDPATHLAAPAEPGSLLAFDDEPSATINAACGSCHADAHTARANP
                     HSQAGLACTDCHSIHPSSAAEIALPAEFDRVDAASATCFECHQETFSQFSFNERHRLQ
                     EGSVSCTSCHDPHAPASSGHLGAFGQRVCAECHADVDGPFVFEHDASRVEGCGACHEP
                     HGSPNRHMLTHQQVGELCYSCHAIVPQFHLGFSPAGPPRFDEGTVCTNCHATIHGSNL
                     DPAFLR"
     gene            2691..3962
                     /locus_tag="AC16VR_11595"
     CDS             2691..3962
                     /locus_tag="AC16VR_11595"
                     /inference="COORDINATES: protein motif:HMM:NF048463.2"
                     /note="uses the energy from reduction of ubiquinone-1 to
                     ubiquinol to move Na(+) ions from the cytoplasm to the
                     periplasm; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit A"
                     /protein_id="HIR6011843.1"
                     /translation="MLIEITTGLDVEVSGRPEQTIDTGPEVGQVALLGGDYLNLRPSL
                     EVDEGARVKAGDTIFSDRARPEIRFVAPAAGRITQINRGPRRTLVSVVIDVDGDERAI
                     VDVPDDPSRREEIVALLLRSGLWPALRSRPFGRVADPGGTPAALLLTAIDTRPLAPDP
                     AVVVGLREADFAAGARALACLAPVTYLCSRAGASLPDVDAEGLSVVEFSGPHPAGLVG
                     THLRHLCPEADEVWHIGYQDVLALGRLLVSGELCDERVVSVAGPGAVRPRLLRTRAGA
                     RLSELVQGESENGCAVIAGSVLSGRAPTPASGYLGRYDSQVSLLRAAGPSRRRWLDVV
                     WPRGAAPARNIGRGSGMLPVESFDRVWPFQAPVAPLLRALLTGDTELASSLDCLSLVE
                     EDLALCAYVCPSRLDYGAALRATLHEIERGR"
     gene            3962..5107
                     /locus_tag="AC16VR_11600"
     CDS             3962..5107
                     /locus_tag="AC16VR_11600"
                     /inference="COORDINATES: protein motif:HMM:NF015100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HIR6011844.1"
                     /translation="MADSTILQRVRGIVIDLADPYRAPEARAAGSPPFLRQALDTPGL
                     LACFVVATLPAWAVGVWNTGRRGLEAGFGSEGESWQLGMLAAFRVDADPTAMLACLTL
                     GVLYFIPLLAAVLATSAFWALVFARTRQGGVDPGWLFSGWLMVLLMPPSTPLTIAVLA
                     ASFGVVLGSHIFGGTGKYLISPALLGVLFLHFSYPELVQTPLAAGDAGSADGWRALAA
                     GGEDVVEFLLGREPGGLATGSALACAVGALYLVRVGAASWRTIAGAVVGFLVAATVVN
                     VSAGEDPAWHVPWYAHLAVGYVAFGVAFLATDPSAAPLTRPARWAHGLLIGVLTVVIR
                     VLDPTHPEGSLYALLLAGLSVPFLDYLVVRRYRTRVRIRAQLRTRGL"
BASE COUNT          728 a         1683 c         1835 g          958 t
ORIGIN      
        1 agaatctcgt cgcccgcgcg gacgtcgtcc cggtcgaccc tgaagctgcc ctcgttctcg
       61 taagctcggt agctggcggt caggtccacc cggtcggtga catgccaacg aacggcgccg
      121 tcccagaaat ccgcatccgc gccgtaggag atggcgaaca gatcctgccg ggagcccccg
      181 gtcaccagct ggtccacttc ccgttggagc tcgacgaagg cggctcccac cgacaccgtc
      241 aggcggtcgc ctgcgtgggt caggcggatg tcggactgac ggctttcgga gatccagccc
      301 gtattgtcgt tttcgtagtc cgtgcgccgg taggacgcgg aaaccttcag gccgttgtcc
      361 caccggtagg atgcgcgcgc cttgtagcgt cgggcatcgg tagccgatgc cagcgtgtag
      421 ggatcgtcga tccggtcgtc ctcgaagctc aaggtgacct gggctcttcg gcttggccgg
      481 tagctgagcc gcccgaagaa gccgtcccgg tcggtatcca cggcttcggt gtggccggcg
      541 ccttcgatgg cgtggctgac gacgctcttc cgccgctcgg tggaccatcc gccgctgagt
      601 gtcaccgtgg tcgtcacggc catgtctacc ccgagctcga acccggtggt ctcgatgtcc
      661 cacgcgctcg cgccggcggt gtcgccgaac agcaccgcgc cgtcctgctc cagatcgtag
      721 cggcgcacgc cggcgttgag ctggagacga tcgttgacca ggtaggcgac cgaggcgtcg
      781 agcagcaggg tgtccctgtc cacgtctgct gcccccgcgt cctcgcggtc gaacggacgc
      841 ccgaggtagt cggtgccctg ggcccgctcc gttgccgtca tgtccaggtc caggttgccg
      901 gcccgcgcgt cgagctccac gatccactgg gatgatggcc gcacctgcac gctcagacgc
      961 tgatcgctgg acgtgaagcc gtagggctgg tccagaaaaa agagatccag cgaggtgggt
     1021 gcgtccgcct cgctgcccgg cgagaacccg ggcagaaaga tctgcgagtc gttgtcgtac
     1081 tcctgccagg tctggcccag ggccacggtg acgtgatccc aagcgtaagt gaagccgagt
     1141 tcgtatctgt ccagggactc gtcgatgggc ttgtcgagct cgaattcctc ccgctccacg
     1201 tccagcacgg tggtgctctc gccgcgtttc tcgtatcggg cgaatcccag gttcagcttg
     1261 gcccggctgc tcagatccag atccagcgcg agggcgtcgc gatttctctc gaagtcgaaa
     1321 tgatggaagt cgccgcctgt ggactgctcg acgctggctt cctcgggtgc gagcagcagg
     1381 tcctcgtaga aatagtccga gctgcgatga tcataggtga gccggtagct tccgtaacgt
     1441 tgaacgcgca cccgcacgga ttcgtagggg tccccgccga gcccgctggc gttgagctct
     1501 acccggtcgg gcatgccggc cgggctattc gcgtcccggt cgggctggaa gaccatgccg
     1561 agatcgaaca ggcgggcccc atcatcgagg ttgacgtgct gttggtactt gccctcgtcc
     1621 ccggacacgt cgacctcgcg gtagccgacg ctcacgtgac cggtgaaggt cgcggcggcg
     1681 gcgctgcggg tacttgccgc accggcgatc agcagcgtga aaacgatcag gagccgagcc
     1741 gtcatgagcc cgtcacctca gaaaggccgg gtccaggttg gacccgtgaa tggtggcgtg
     1801 gcagttcgtg cacacggtgc cttcgtcgaa tcggggcggg cccgcggggc tgaagccgag
     1861 gtggaactgg gggacgatgg cgtgacacga gtagcagagc tcgcccacct gctggtgcgt
     1921 gagcatgtgc cgattcgggc tgccgtgggg ctcgtggcag gcgccgcatc cttccacacg
     1981 ggacgcgtca tgctcgaaca cgaacgggcc gtcgacgtcg gcgtggcact cggcgcacac
     2041 cctctggccg aaggcgccaa gatgcccgga gctcgccggc gcgtgggggt cgtggcagga
     2101 cgtgcaggaa acgctgcctt cctgtaggcg atgacgctcg ttgaaggaga actggctgaa
     2161 tgtctcctgg tggcattcga agcacgtggc cgaggccgcg tcgacgcgat cgaactcggc
     2221 aggcagagcg atttcggctg cgctcgatgg gtgaatgctg tggcaatccg tgcaagcgag
     2281 cccggcctgg gagtgcgggt tggcgcgagc ggtgtgagcg tccgcgtgac agctgccaca
     2341 ggcggcattg atcgtcgcgc tgggctcgtc atcgaatgcg agcagggatc ccggctccgc
     2401 gggtgcggcg aggtgagttg ccggatcgcc gtggcaactc ctgcatccgg acgcttcgtc
     2461 gtcctccgtc cgggccccgt ggggagtacc gtggaagtcc gccacgagct cgccgtggca
     2521 agcggcgcag gtcgggtttc cgccgacctg gccggttgcc agcagaaaca gagtgcccac
     2581 cgccattgaa aacaaacgca tgggtttagg gtccagcagc acgtgtcgct ctgctattgt
     2641 gagttctacg tagagatact acccgagggt cgtgtcagtt tggtgccggc atgctgatcg
     2701 agatcaccac aggccttgac gtcgaagtct ccggtcggcc ggagcagacc atcgatacgg
     2761 gtccggaggt cggccaggtt gccctgcttg gtggggacta cctgaacctt cgcccgtcgc
     2821 tcgaggtgga cgaaggtgcc cgggtcaaag cgggtgatac gatcttcagc gaccgggcgc
     2881 gtccggaaat tcgcttcgta gcaccagccg ccggccggat cacccagatc aatcgtggac
     2941 cacggcgcac gctggtctcg gtggtgatcg acgtcgatgg ggatgagcgg gcgatcgttg
     3001 atgtgccgga tgacccgtca cggcgcgagg agatcgtcgc gctgctcctg cgttcgggcc
     3061 tttggcccgc cctcagatcg cgacccttcg gtcgcgtggc cgatcccgga gggacaccgg
     3121 ccgcgctgct cctcacggcc atcgacaccc ggcccctggc cccggatccg gccgtggtcg
     3181 tcgggcttcg agaggctgac tttgccgccg gcgcccgggc gctcgcctgt ctggcgccgg
     3241 tcacctatct ctgctcccgg gcaggggcca gcctcccgga cgtcgatgcc gagggcttga
     3301 gcgtggtgga gttcagcggc ccgcatccgg ccggcctggt gggtacccat ctccgccacc
     3361 tgtgcccgga ggcggacgag gtctggcaca tcgggtatca ggacgttctg gcactcggac
     3421 gattgctcgt ctcgggcgag ctttgcgatg aacgagtggt ttcagtggcc gggccgggag
     3481 cggtcaggcc gcggctgctg cgcacgcggg cgggtgcgcg cctgagcgag cttgtgcagg
     3541 gcgagtcgga aaacggctgt gccgtgatcg ccgggtccgt tctgagcgga cgcgcaccga
     3601 cgccggcgag tgggtatctt ggccggtacg attctcaggt atcgctgctg cgcgcggcag
     3661 gtccgtcacg acgccggtgg ctggatgtgg tttggccgcg cggggccgct ccggcgagga
     3721 acatcggtcg tgggagcggg atgctacccg tcgagagctt cgaccgcgtc tggccgttcc
     3781 aggcccccgt cgcgccgctc cttcgtgcgc tgctcacggg tgacaccgag ctcgcgagct
     3841 ccctggattg cctgtctctg gtcgaggaag acctggccct gtgcgcatac gtgtgcccat
     3901 cgcgactaga ctacggggcc gcgctgcgtg cgaccctgca cgagatcgag cggggccgct
     3961 gatggctgac tcgacgatcc ttcagcgggt caggggaatc gtgatcgacc tcgcggatcc
     4021 ctaccgcgca cctgaagccc gcgcggcggg atcgccgccc tttctgcgcc aggcgctgga
     4081 cactccggga ctgctcgcct gcttcgtagt ggctaccctg ccggcctggg ccgtcggtgt
     4141 ctggaacacc ggtcggcgcg gactggaagc agggttcggg agcgagggcg aaagctggca
     4201 gcttggcatg ctggccgcgt tccgcgtcga tgcggatcca acggccatgc tggcctgcct
     4261 gacgctcggg gtgctgtatt tcatcccgct acttgcggcc gtcctggcca ccagtgcctt
     4321 ctgggccctg gtttttgcga gaacccggca gggcggcgtc gaccctggct ggctgttcag
     4381 cgggtggctc atggtgctgc tcatgccgcc gtcgaccccg ctgaccatcg cggtcctggc
     4441 ggcgagcttt ggcgtggtgc tcggtagtca catctttggc gggacgggga aatacctgat
     4501 cagcccggcc ctgctcggcg ttctgttcct gcacttcagc tatcccgagc tggtacagac
     4561 cccgctcgcc gctggcgatg cggggagcgc agacgggtgg cgcgcgctgg ccgcgggcgg
     4621 cgaggacgtc gtcgaattcc tcctggggcg cgagccggga ggcctggcga ccggctccgc
     4681 gctcgcctgc gccgtcggag cgctctatct ggtgcgcgta ggcgccgcct cctggcgcac
     4741 gatcgcgggc gccgtcgtgg gcttcctcgt cgcggccacc gtcgtcaacg tctcggcggg
     4801 ggaagacccc gcctggcatg tgccctggta cgctcatctg gcggtcggct acgtggcttt
     4861 cggggttgcc tttctggcca ccgatcccag cgctgcgcca ctgacccggc cggcccggtg
     4921 ggcgcacggt ctgctgatcg gcgtgctcac ggtagtgatc cgcgtgctgg atccgaccca
     4981 cccggaaggc tctctgtacg cgctcctgct ggcggggctc agcgtgccgt ttctggacta
     5041 cctggtggtg cggaggtacc gcacgcgggt gcggattcgg gcgcagctgc ggacgcgggg
     5101 cctctgatcg tggcgcacga accgatttcc cgcacgctgg ctgtcgctgt ggccatagcg
     5161 ctggtctgct cggtcatggt ttctaccgcc gtctacctgc ttcg
//
LOCUS       DBNHQR010000511         3600 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_50481, whole genome shotgun sequence.
ACCESSION   DBNHQR010000511 DBNHQR010000000
VERSION     DBNHQR010000511.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_50481"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..1179
                     /gene="coxB"
                     /locus_tag="AC16VR_11605"
     CDS             64..1179
                     /gene="coxB"
                     /locus_tag="AC16VR_11605"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008569177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HIR6011845.1"
                     /translation="MGLGLAAAARADELNMTPGVTQISRSIYDLHMVIFWICVAIGVV
                     VFGVMFWSIFQHRKSRGAQAASFHESTRLEIAWTLIPILILIGMAFPATQTLVAMYDT
                     GGEDMAVEVRGYQWKWQYKYLDEDYNSTFSFFSNLATSRDEIENRVVKNDNYLLEVDE
                     PLRIPANRKVRFLITSEDVIHAWWVPDFGIKRDAIPGMLNELWTVVETPGVYRGQCTE
                     LCGMDHGFMPIVVEVMAEEDYDAWYADRVRMEEARHEALSKTFTPAELMEEGERVYAT
                     FCASCHQANGKGLPPVFPALAGSPIATGPLSDHLDIIVNGRAGTSMNAFGNQLDAAEI
                     AAVTHYERNAWGNDVGDVTQPIDVIEFMSGKEAEETP"
     gene            1176..2777
                     /gene="ctaD"
                     /locus_tag="AC16VR_11610"
     CDS             1176..2777
                     /gene="ctaD"
                     /locus_tag="AC16VR_11610"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HIR6011846.1"
                     /translation="MSEAVLTHHDDHHHGPASGITRWLFTTNHKDIGTLYLWFSFLML
                     LIGGAMALVIRTELFQPGLQFVEPAFFNQMTTNHGLIMVFGAIMPAFVGLANWLLPMM
                     VGAPDMALPRMNNLSFWILPFAFGMLISTLFMEGGGPNFGWTFYAPLSTTYAPDTVTY
                     FIFGVHLMGASSIMGSINIIATILNMRAPGMTLMKMPLFVWTWLITAYLLLAVMPVLA
                     GAVTMMLFDIHFGTSFFNAAGGGDPVLFQHIFWFFGHPEVYIMILPAFGVVSQIIPTF
                     ARKPLFGYDSMVYATASIAFLSFIVWAHHMFTVGMPLAGQLFFMYATMLIAVPTGVKV
                     FNWVSTMWRGSMTFETPMLFAIAFVVLFTIGGFSGLMLAIAPADYQYHDTYFVVAHFH
                     YVLVPGALFSIIAAVYYWLPKWCGRMYDETLGKVHFWLSFIGVNVTFFPQHFSGLAGM
                     PRRIPDYALQFADFNMVSSVGAFIFGFSQLVFLYVVIKAIRSGRPATDRVWEGAHGLE
                     WTLPSPAPYHSFTTPPTVTEAEAHS"
     gene            2817..3353
                     /locus_tag="AC16VR_11615"
     CDS             2817..3353
                     /locus_tag="AC16VR_11615"
                     /inference="COORDINATES: protein motif:HMM:NF003465.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HIR6011847.1"
                     /translation="MHKSSKGIVWKLGLMVVGMFGFGFALVPLYDLLCEVTGLGGRTG
                     GPYAYDPAVVEVDRSRLVKVNLLTNTNDGMPWRFWSEVGGMRVHPGEAKDVKFYVKNT
                     TDRRMVGQAVPSVVPITATDYFHKTECFCFESQVLEPGEEMELPLRFIVDAGLPKNVQ
                     TISLSYALFDATGFAAAE"
     gene            3382..>3600
                     /locus_tag="AC16VR_11620"
     CDS             3382..>3600
                     /locus_tag="AC16VR_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011848.1"
                     /translation="MASSSDQATYYIPHSGWYPIWVAVGSALLLFGLGSWLNDLKAET
                     EPSRALFYTGAVILSVVLFAWFAKVVNEN"
BASE COUNT          640 a         1106 c         1118 g          736 t
ORIGIN      
        1 cacaacctaa cgcaagggtg agcaggtttg aaacattact ggcatgggct gccgacgtgc
       61 ctgatgggcc ttggactggc cgccgctgcg cgagcggacg agctgaacat gacgccggga
      121 gtgacgcaga tcagtcgctc catctacgac cttcacatgg tgatcttctg gatctgcgtc
      181 gccatcggcg tggtggtttt cggggtcatg ttctggtcga tttttcagca caggaagtcg
      241 cggggcgcgc aggcggccag cttccatgaa agcactcgcc tggagattgc ctggacgctg
      301 attccgatcc tcatcctcat cggcatggcc tttccggcca cccagaccct cgtcgccatg
      361 tacgacacgg gcggagagga catggcggtc gaggtgcgtg gctatcagtg gaagtggcag
      421 tacaagtatc tggacgagga ctacaacagc acgttcagct tcttcagcaa cctggcaacc
      481 tccagggacg agatcgaaaa ccgtgtggtg aagaacgaca actacctcct ggaggtcgat
      541 gagccgctca ggatcccggc gaatcggaag gtgcgctttc tcattacctc ggaggacgtg
      601 atccacgcgt ggtgggttcc ggatttcggc atcaagcgag acgcgatccc aggcatgctc
      661 aacgagctgt ggaccgtcgt ggagacgccg ggcgtttacc gggggcaatg cactgagctt
      721 tgcggtatgg accacggctt catgcccatc gtcgtcgagg tcatggcgga ggaagactac
      781 gacgcctggt atgcggaccg ggtgcgcatg gaggaagcgc gccacgaagc gctctccaag
      841 acgtttacgc cggcggagct catggaggag ggggagcgcg tctacgcgac gttctgcgca
      901 agctgtcatc aggcaaacgg caaagggctg ccaccggtgt tcccggccct tgccggaagc
      961 cctatcgcca cggggccgct cagcgaccat ctggacatca tcgtcaacgg ccgtgccggg
     1021 acgagcatga atgcctttgg caaccagctg gacgcggcgg agatcgcggc cgtgactcat
     1081 tacgagcgga acgcctgggg taacgacgtg ggcgacgtta cgcagcccat cgatgtcatc
     1141 gagttcatgt ctgggaagga agccgaggaa acgccatgag tgaagccgta ctgactcatc
     1201 acgacgacca tcatcacggg cccgcttccg gaatcacccg ctggctgttc accacgaacc
     1261 acaaggacat cggtaccctc tacctctggt tcagcttcct gatgctgctc atcggcggcg
     1321 ccatggcgct ggtgatacgt acggagctgt tccagccggg gctgcagttc gtggaacccg
     1381 ccttcttcaa ccagatgacc acgaaccacg gcctgatcat ggtcttcggg gccatcatgc
     1441 cggccttcgt cggtctggcg aactggctgc tgccgatgat ggtgggcgcg cccgacatgg
     1501 cgctgccgag gatgaacaac ctgtcgttct ggatcctgcc gttcgccttc ggcatgttga
     1561 tctcgacgct gttcatggag gggggcgggc cgaatttcgg atggacgttc tacgcccccc
     1621 tgtcgacgac ctatgcgccg gacacggtga cctatttcat tttcggcgtt cacctgatgg
     1681 gtgcgtcctc catcatgggg agcatcaaca tcatcgccac catcctgaac atgcgcgcgc
     1741 cgggcatgac gctcatgaag atgccgctgt tcgtctggac ctggctgatt actgcctatc
     1801 tgctgctggc cgtcatgccg gtgctggccg gggcggtcac catgatgctg ttcgacatcc
     1861 acttcgggac gagctttttc aacgcggccg gcggcggtga ccccgtgttg ttccagcaca
     1921 tcttctggtt cttcgggcac ccggaggtct acatcatgat cctgcccgcg ttcggcgtcg
     1981 tctcccagat catccccacg ttcgcgcgca agcctctgtt cgggtacgac tcgatggtct
     2041 acgccaccgc cagcatcgcc tttctgtcgt tcatcgtctg ggctcaccac atgttcaccg
     2101 tgggcatgcc gctggccggg cagctgttct tcatgtacgc aaccatgctc atcgcggtgc
     2161 ccaccggggt aaaagtgttc aactgggttt ccaccatgtg gcgtggatcc atgacgttcg
     2221 agacgcccat gctgttcgcc atcgcgttcg tcgtgctgtt caccatcggc gggttctcgg
     2281 gcctgatgct cgccatcgcc cccgcggact atcagtacca cgacacctat ttcgtggtgg
     2341 cgcacttcca ctacgtgctc gttccgggcg cgctgttctc catcatcgcc gccgtctact
     2401 actggctgcc caagtggtgc ggccggatgt acgacgagac cctgggcaag gtgcacttct
     2461 ggctgtcgtt catcggggtc aacgtgacgt tcttccctca gcacttctcg ggcctcgccg
     2521 gcatgccgcg gcgaatcccc gactacgcgc tgcagtttgc ggacttcaac atggtctcca
     2581 gcgtcggggc gttcatcttc ggtttctccc agctggtctt cctctacgtg gtgatcaaag
     2641 ccatcaggag cgggcggccg gccaccgacc gagtctggga gggcgcccat gggctggaat
     2701 ggacgctgcc ctcgccggcg ccctaccaca gcttcacgac tccgcccacc gtcaccgagg
     2761 ccgaagccca ctcctgatgg gggaggggct aatcgggtag gaggcagcga agccgcatgc
     2821 acaagagcag caaaggcatc gtatggaaac tcgggctgat ggtcgtgggc atgttcggct
     2881 tcggcttcgc cctggtcccg ctttacgatc tgctgtgcga ggtcaccggc ctgggcggca
     2941 ggaccggtgg gccctacgct tacgacccgg cggtcgtgga ggtggatcgc tcccgactgg
     3001 taaaggtcaa cctgctgacc aataccaacg atggcatgcc ctggcggttc tggtccgagg
     3061 tggggggcat gcgcgtgcat cctggtgagg ccaaggacgt gaagttctac gtcaagaaca
     3121 ccaccgatcg gcgcatggtg ggccaggcgg tcccctcggt ggtcccgatc acggcaaccg
     3181 actattttca caaaactgaa tgcttctgtt tcgaaagcca ggtcttagag cctggcgagg
     3241 aaatggagct gccgctgcga ttcatcgtcg atgcggggct gccgaagaac gtgcagacca
     3301 tatcgctcag ctacgccctg ttcgacgcca ccggtttcgc ggcggcggag taacccgaca
     3361 ccgaaaaggg agagaacggc catggccagc tcatcagacc aagccactta ttacatcccc
     3421 cacagcgggt ggtacccgat ctgggtggcc gtcgggtcgg ccctgcttct gttcgggctg
     3481 ggctcctggc tgaacgatct caaggccgag acggagccga gccgcgcgct gttctatacc
     3541 ggggccgtca tcctgtctgt ggtgctgttc gcctggtttg caaaggtggt caacgagaat
//
LOCUS       DBNHQR010000512         4981 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1378406, whole genome shotgun sequence.
ACCESSION   DBNHQR010000512 DBNHQR010000000
VERSION     DBNHQR010000512.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4981)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4981)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4981
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1378406"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="AC16VR_11625"
     CDS             complement(<1..579)
                     /locus_tag="AC16VR_11625"
                     /inference="COORDINATES: protein motif:HMM:NF013528.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIR6011849.1"
                     /translation="MTQTGPGPQIVTRPGADADRLPHLPPLLARIYAARGIVEAADVD
                     HALAGLHAPDTLPDIQRAGQRLARAVMEDERILVVGDFDADGATSVALALTLLRALGA
                     AQVEFMVPNRFDFGYGLSPEIVDLAARRQPAVLVTVDNGVSSVAGVARANEHGIDVIV
                     TDHHLPGDVRPDAYAIVNPNLPESAFPSRSVAG"
     gene            complement(576..1892)
                     /locus_tag="AC16VR_11630"
     CDS             complement(576..1892)
                     /locus_tag="AC16VR_11630"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015885695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HIR6011850.1"
                     /translation="MEPLKVGMCGLGTVAQGVLEVLATNRESITRRAGREIRVTRVAS
                     RRAKPDVDLGRAEFSTDVHALARDPDIDVLLELIGGEDTALELVTSALARGTPVVSGN
                     KAIVALHGNELLPLAAQHGAHLGFEAAVAGSIPIVSSLNRSLAANQHEWLAGIINGTS
                     NFILSAMTQSGRAFADALAEAQTLGYAEADPTFDVEGIDAAHKLTILTALAFDVGFDF
                     HSVYTEGISKLTAEDIGYARQLGYRIKHLGIARRREHGIEARVHPVLVPEQHLLASIG
                     GAMNAVLVHSNAAGDTLYSGAGAGALPTASAVVADLIDLARGTARLPSPVGDVDLPIL
                     PIAETDSAYYLRIPSVDRPGAFARVATILSEREISIEAAIQREEARVRTRGAESGDGA
                     WVPIVILTHSVREAVMNEALEAVQALPDVVGEIMRIRVEHFDEDGE"
     gene            complement(1986..2720)
                     /locus_tag="AC16VR_11635"
     CDS             complement(1986..2720)
                     /locus_tag="AC16VR_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003756 - protein disulfide isomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HIR6011851.1"
                     /translation="MLACLAVIGHADEAAEADEVKDVTDAKGGQALVERLTALRPGIP
                     IEQVRETPLAGIYALELKGGSFFYGTADGKYLFAGDMYELTDTSLVNLAEAGRTAKRQ
                     ALMAQVTAEEMVVFSPEGTTKASISVFTDVDCGYCQKLHLEVPKLNEMGIEVRYLAYP
                     RAGVGSQSYQKIVSAWCSADRNTAITRLKARQTIPTLTCSNPVASQLDLGREVGVTGT
                     PAIVLEDGRLLPGYMPAEQLAEALGI"
     gene            complement(2880..3785)
                     /gene="xerD"
                     /locus_tag="AC16VR_11640"
     CDS             complement(2880..3785)
                     /gene="xerD"
                     /locus_tag="AC16VR_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HIR6011852.1"
                     /translation="MGEVLPKDEAARHTVDEYLDAVWLERGLSENTLAAYRRDLYQLA
                     DMLKRDPLTANDADLLECLALRHRQGYRASSAARWLSSVKGFYRHAAGRGRIGADPSA
                     HLEHPKMGRRLPGSLSEAEVRQLLAAPRLDDPIGLRDKAMLELLYASGLRISELVGLT
                     MSAVNARQGIVRIVGKGGKERLVPMGGEAMRWLERYLHEGRPLIKGSDATAAMFPSRR
                     GSQMTRQNFWHAVKRYAMKAGIHRSISPHTLRHAFATHLINHGADLRAVQMMLGHADL
                     STTQIYTHVAQARLKDLHRTHHPRG"
     gene            complement(4129..4479)
                     /gene="rplS"
                     /locus_tag="AC16VR_11645"
     CDS             complement(4129..4479)
                     /gene="rplS"
                     /locus_tag="AC16VR_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010220244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HIR6011853.1"
                     /translation="MRRNKVIEELESEQLKSDLPEFGPGDTVVVQVKVREGNRERLQA
                     FEGVVIGKRNRGLNSAFTVRKMSHGVGVERTFQSHSPMIASVSVKRRGDVRQAKLYYL
                     RERSGRSARIKEKV"
     gene            complement(4486..>4981)
                     /gene="trmD"
                     /locus_tag="AC16VR_11650"
     CDS             complement(4486..>4981)
                     /gene="trmD"
                     /locus_tag="AC16VR_11650"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003092638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (guanine(37)-N(1))-methyltransferase"
                     /protein_id="HIR6011854.1"
                     /translation="QGRRLTQQRVSELSQQRGVILLAGRYEGIDERAVSLAVDEEVSI
                     GDYVLSGGELPALVVLDAVTRLLPGTLGNSASIEAESHLDGLLDYPHYTRPENTHGLG
                     VPEVLLSGDHEAVSRWRRKMALLRTWQRRPDLLVGRQLDDEDSALLRECFEELAARTE
                     SGEE"
BASE COUNT          877 a         1684 c         1522 g          898 t
ORIGIN      
        1 cccggcgacc gaccggctcg gaaaggccga ctcgggaaga ttaggattga cgatggcgta
       61 cgcatccggc cgcacgtcgc cgggcagatg atgatcggtg acgatcacgt cgatgccatg
      121 ctcgttggcg cgtgccaccc ccgcgacgct ggacacgcca ttgtcgacgg taaccagaac
      181 ggccggctgc cttcgcgcgg cgagatcgac gatctccgga gaaagcccgt acccgaagtc
      241 gaaccggttc ggcaccatga actccacctg cgccgccccg agggcgcgaa ggagagtaag
      301 ggcgagcgcc acggacgtag cgccgtcggc gtcgaagtcg cccaccacga gaatccgctc
      361 atcttccatg accgcacggg caagacgctg accggcccgc tgaatgtcgg gcagcgtgtc
      421 gggcgcgtgg agcccggcga gcgcatgatc gacgtctgct gcctcgacga taccccgcgc
      481 ggcgtagatc ctcgcgagca gcggcggcag gtgcgggagc cgatccgcgt cggctcccgg
      541 acgggtaacg atctgcgggc caggccccgt ctgcgtcatt cgccgtcttc gtcgaagtgc
      601 tctacccgga tgcgcatgat ctcgccgacg acgtccggca gagcctgcac ggcctcgagc
      661 gcttcattca tgacggcctc gcgcacgctg tgggtcagaa tcacgatagg cacccaggct
      721 ccatcgcccg attccgctcc gcgggtccga actctggctt cttcccgctg aatggccgct
      781 tcgatgctga tctcccgttc gctgagaatg gtcgcaaccc gggcgaacgc gcccggacgg
      841 tcgacggagg gaatccgcag gtaataggcg ctgtcggtct cggcgatcgg cagaatggga
      901 aggtcgacgt ctccgaccgg gctgggcaat ctggcagtgc cacgggcgag gtcaatgagg
      961 tcagcgacca cggctgaggc cgtcggcagc gcgccggctc ccgcaccgct gtagagcgta
     1021 tcgcccgcgg cattgctgtg caccaggacg gcgttcatcg caccgccgat actggcgagc
     1081 agatgctgct ccggcaccag cacggggtgc acgcgagctt cgatgccgtg ctcgcggcgc
     1141 cgcgctatgc cgaggtgctt gatgcgatag ccgagttgcc gggcatagcc gatatcctcg
     1201 gctgtcagct tcgagatgcc ctccgtatag accgagtgaa agtcgaagcc gacatcgaag
     1261 gcgagcgccg tgaggatcgt cagcttgtgg gcagcgtcga tgccctccac gtcgaaggtg
     1321 gggtcggctt cggcgtagcc gagggtctgc gcctcggcca gcgcatcggc gaaggcccgc
     1381 ccactctggg tcatggcgct gagaatgaaa ttggaggttc cgttgatgat gccggccagc
     1441 cattcatgct ggttggcggc cagagaccgg ttgaggcttg aaacgatcgg tatgctgccc
     1501 gctaccgcgg cttcgaaccc gaggtgcgcg ccgtgctggg ctgccaacgg caggagctcg
     1561 tttccgtgca gcgccacgat ggctttgtta cccgaaacca ccggcgttcc cctggcgagg
     1621 gcactggtga cgagctccag ggcggtatcc tcacccccaa tgagctctag gagaacatcg
     1681 atgtcgggat ccctggcgag ggcatgcacg tcggtggaaa actcggcccg gccaaggtcc
     1741 acgtccggct tggcccgccg gctggccacg cgggtcaccc gtatttcccg gcccgcccgc
     1801 cgggtaatgc tttccctatt ggtggccagc acctccagca cgccctgtgc cacggtgcct
     1861 agaccacaca ttcctacctt gaggggttcc aacgccaatg accaagatgc tgcgacgagg
     1921 cgccaagtct actggcgcgc cgtcctgtgc tcaaaggtat cgacgggaaa cttcgcccga
     1981 ggggatcaga tgcccagcgc ttccgccagc tgctcggccg gcatgtagcc gggcagcagc
     2041 cgcccgtcct cgagaacgat ggccggtgtg cccgtcacgc ccacttcgcg cccgaggtcg
     2101 agctgggaag ccaccgggtt tgagcaggtc agcgtgggaa tggtctgtcg cgccttcagc
     2161 ctggtaatgg ccgtgttgcg atcggccgag caccaggccg acacgatttt ctgataggac
     2221 tgagaaccca ccccggcccg cggataggcc aggtaccgga cctcgatgcc catctcgttg
     2281 agcttgggca cctcgaggtg cagcttctga cagtaaccac agtccacgtc cgtgaacacg
     2341 ctgatggacg ccttggttgt cccttccggg gagaaaacca ccatctcctc cgccgtcacc
     2401 tgggccatca gggcctgccg ctttgccgtt cgccccgctt cggcgaggtt caccaggctg
     2461 gtgtcggtga gctcgtacat gtctccggcg aacagatact taccatccgc ggtaccgtag
     2521 aaaaacgaac cgcccttgag ctcgagggcg taaatgccgg cgagcggggt ttcgcgtacc
     2581 tgctctatgg gtataccggg acgcagcgcc gtcagccgct cgacgagcgc ctggcctcct
     2641 ttggcgtccg tgacgtcctt gacctcatcg gcctcggctg cctcgtcggc gtgtccgatg
     2701 acggccaggc acgctagcac tgccccgacc cagagggtcc gaaatgacaa cttcattgat
     2761 ttcttcacgg tgcctcctgc acggccggag aattagacca gcgagcgcgc ccaaatatcc
     2821 agcttaacca cattcctttc caaatagcct gctccaatac tcaaacccgc gcttcgctct
     2881 catcctcgcg ggtgatgggt ccgatgcagg tccttcagcc gagcctgcgc gacgtgcgtg
     2941 tagatctggg tcgtggacag gtccgcatgt cccagcatca tctgcacggc cctgaggtcc
     3001 gccccgtgat tgataaggtg ggtggcaaag gcatggcgca gcgtatgcgg cgagatgctc
     3061 cggtggatgc ccgccttcat ggcatagcgc ttcaccgcgt gccagaagtt ctgtcgggtc
     3121 atttgcgagc cccggcggct cggaaacatc gctgccgtgg catcgctgcc cttgatgagc
     3181 ggacggccct cgtggagata acgctcgagc cagcgcattg cctccccacc catgggcacc
     3241 aggcgctcct tgcccccctt cccgacgatt ctcacgatcc cctgccgcgc gttcacggcg
     3301 ctcatcgtga gacccaccag ctcggatatc ctcaaccccg aggcgtagag aagttccagc
     3361 atggccttgt ccctgagccc gatgggatcg tccaggcgcg gtgctgccag cagctgacga
     3421 acttccgcct ccgacaacga gcccggcagc cggcgtccca tcttcggatg ctccaggtga
     3481 gcgctgggat ccgcgccgat ccgcccgcgc ccggccgcgt gccggtagaa gcctttcacg
     3541 gaggagagcc agcgggcggc ggagctggcg cggtaaccct gacgatgccg cagcgccagg
     3601 cactcgagca gatccgcgtc gttggctgtc agcggatccc gcttcagcat gtcggcaagc
     3661 tggtagagat cccgacgata ggcggccagc gtgttctcgc tcaagccccg ctcgagccag
     3721 accgcgtcga gatattcgtc caccgtatgg cgggccgcct catccttcgg cagaacctcg
     3781 cccatttcgt ctccttgggc acccatcacg gacactttgt gacgtagttg gtcctcagtc
     3841 aaccgacata aaaaccagcg gatagttatt caactacatg aaacaaaagg atttttaatc
     3901 tattttgaac ggaatccaga tagctggcac ggtagctgca ttggcttata cgggcgcgat
     3961 ccacccaccc agctgtcccc tggagtgatc gtgacccacg ctgaaggaag ccggccccct
     4021 ctccaccggt ttccgaagac ggccagcttg ccggggcctc cccccaactc ccccaacccg
     4081 gcaagctggc ttcatttttt ttaagccgcc gggcggtgaa acgacgggtt agaccttttc
     4141 cttgatgcgc gcagaccggc ccgagcgctc acgcaggtag tagagcttgg cctgacggac
     4201 gtcgccgcga cgtttgacac tgaccgaagc gatcatcggg ctgtgactct gaaacgtgcg
     4261 ctcgacaccg accccatggg acatcttgcg aaccgtgaat gccgagttca gcccgcgatt
     4321 gcgcttgccg atcaccacgc cttcgaaggc ctgcagccgt tctcggttac cctcacgaac
     4381 cttgacctgc acgacgaccg tgtctcccgg cccgaactcc ggcaggtcgg acttcagctg
     4441 ctccgactcg agctcctcga tgactttgtt tctgcgcacg atcttctact cctcgcccga
     4501 ctcggtccga gcggccaatt cttcaaaaca ctcgcgcaag agcgcgctgt cctcatcatc
     4561 cagctgccgc ccgaccagca gatcggggcg ccgctgccag gtccgcagca aggccatctt
     4621 ccgccgccag cggctcactg cctcgtggtc tccgctgagc aggacctcgg gcacacccag
     4681 gccgtgggtg ttctcggggc gcgtatagtg agggtaatcg agcaatccat caaggtgtga
     4741 ttcagcctct attgaggccg aattgccgag agtccccggc agcagccgcg tgacggcgtc
     4801 cagcacgacc agcgcgggca actcgccgcc gctgagaacg tagtcgccga tggaaacttc
     4861 ctcgtccacc gccagcgaga cggccctctc gtcgatgccc tcgtaacgac ccgccagcag
     4921 tatcaccccg cgctgctgag acaattcact gactctctgc tgcgtcagcc gccgaccctg
     4981 g
//
LOCUS       DBNHQR010000513         2932 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1000599, whole genome shotgun sequence.
ACCESSION   DBNHQR010000513 DBNHQR010000000
VERSION     DBNHQR010000513.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1000599"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="AC16VR_11655"
     CDS             <1..1049
                     /locus_tag="AC16VR_11655"
                     /inference="COORDINATES: protein motif:HMM:NF012385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR6011855.1"
                     /translation="GDSAPIRWLHRDERYAEKLATPDITIADLIGEVDPIKVAEGRYL
                     SDELTLHYGLVPRVNRGIFAINELPDLSERIQVGLLNLLEERDVQIRGHKVRLPLDVF
                     IVATANPEDYTNRGRIITPLKDRYGAQIRTHYPADVDQEVAIMEQEAAVADMGDYRLE
                     IPAFMKEIVAEITHHARRSPDINQRSGVSVRTSIADYEAMLANAFRRALRLGEDEVVP
                     RISDLAFVLPSFQGKVEFEAMEEGQEDKITQRIMQAAIKSVFDRYYDVNDLESIALAF
                     NDGLTVDTGEATPAADYNAIGERIPGLMDVFPDREGVSPGVRAAIIEFILEGLHLHKL
                     LNKFDNRGAATYSG"
     gene            1080..>2932
                     /locus_tag="AC16VR_11660"
     CDS             1080..>2932
                     /locus_tag="AC16VR_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR6011856.1"
                     /translation="MRHFPSGYRFSRWDGSQKLPLDADEIMAALADDLMEYGDLRWAM
                     RNLMSRGMQIPQGGYMQGLRDMLRQLRERKREQLERFDLSSVFEDFRERLDEILAMER
                     KRIDEWLDPKPATDSEAPAGNGPSGEPEQSTSADQPAAQGDGPDFSGDVLKQIAQRNR
                     DELDGLPPDLAGQVRTLEKYEFLNPDAQRKFLELLNELRRAMTNTFFNDIENMVNQLS
                     DGDVERMKEMIKALNEMLVKRIAGEDPGFEDFMAQYGDMFGDDPPQSLDELLERMAQQ
                     MAAAQSLLNSMSPEQQQQLQSLLSDRFGDPELNSELRKLAKEMDFLNPDGGKYRFSGD
                     ERLDLEAAMKLMSEMQDLDELISQVQEAERGGGTDRIDRDLLSDLLGDAAADDLEQLQ
                     QMLKALEEAGYIRPTDDDGWEITPRGSRMIGQKALGEIYARLKRQNLGNHAVPEEGRF
                     GERLEQTKAYEFGDPFHLHMPRTLRNAIDREGPQTPVSLKPEDFEIYRSELITSTATA
                     MLVDLSWSMALRGSFQAAKKVALALHNLITSQYPKDTFYIIGFAAYAKELKPKDLPYL
                     QWDEYVLGTNMQHALLLAERLLAKHQAGSKQIIMISDGEPTAHLENGQAQFA"
BASE COUNT          598 a          959 c          921 g          454 t
ORIGIN      
        1 agggggacag cgcccccatt cgctggctgc accgggacga gcgatacgcc gagaagctcg
       61 ccactccgga catcaccatc gcggatctca tcggcgaggt cgaccccatc aaggtggccg
      121 aaggccgcta tctctccgat gagctcaccc tgcactacgg gctcgttccg cgcgtcaacc
      181 ggggcatatt cgccatcaac gagctgcccg acctgtccga gcgcattcag gtgggacttc
      241 tgaatctcct cgaagagcgc gacgtgcaga tcaggggtca caaggtccgg ctgccgctgg
      301 acgtcttcat cgtggccacc gcgaaccccg aggactacac caaccgcggc cgcatcatca
      361 ccccgctgaa ggaccgctac ggcgctcaga tccggaccca ctacccggcg gacgtcgatc
      421 aggaagtggc catcatggag caggaagcag ccgtggccga catgggcgat taccggctcg
      481 aaatccccgc cttcatgaag gagatcgtcg cggagatcac ccatcacgcc cggcgctcgc
      541 ccgacatcaa ccagcgatcg ggcgtttccg tgcgaacgtc catcgccgat tacgaagcga
      601 tgctggcgaa cgccttccgg cgggcgctgc gcctcggcga ggacgaggtg gtgccgcgca
      661 tctccgatct ggcgttcgtg ctcccgtcgt ttcagggcaa ggtcgagttc gaggccatgg
      721 aggaaggcca ggaggacaag atcacccagc ggatcatgca ggccgccatc aagtcggttt
      781 tcgatcgcta ctacgacgtg aacgatctgg agtccattgc cctggctttc aacgacgggc
      841 tcacggtgga taccggcgag gccacacccg cagcggacta caacgccatc ggcgagcgca
      901 tacccggcct gatggacgtg tttcccgacc gtgagggcgt aagccccggc gtgcgggcgg
      961 ccatcatcga gttcatcctg gaggggctgc acctgcacaa gcttctcaac aagttcgaca
     1021 acaggggagc tgccacctac tcggggtgag cggcaaccca caccacagct gaggccgtca
     1081 tgcgtcactt tccctcaggc taccgattct cccgctggga cggctcccag aagctgccgc
     1141 tggacgccga cgagatcatg gccgcgctgg ccgacgacct gatggaatac ggcgaccttc
     1201 gctgggccat gcgcaacctc atgtcacgcg gcatgcagat acctcagggc ggctacatgc
     1261 agggcttgcg ggacatgctc cggcagctga gggaacgcaa gcgggagcag ctcgagcgct
     1321 tcgacctgtc gagcgtgttc gaggacttcc gggagcggct cgacgagatc ctcgccatgg
     1381 agcgaaagcg catcgacgag tggctcgatc cgaagcccgc aacggattcc gaagctcccg
     1441 ctggcaacgg gccgagcggc gaacccgagc agagcacgtc tgccgaccag cctgccgccc
     1501 agggggacgg tcccgatttc tccggcgacg tcctcaagca gatcgcccag cgcaaccggg
     1561 acgagctcga cgggctgccg ccggatctcg ccgggcaggt ccgaaccctg gagaagtacg
     1621 agtttctgaa ccctgacgcc cagcgaaagt tcctggagct gctcaacgag ctgcgccggg
     1681 ccatgacgaa cacgttcttc aacgacatcg agaacatggt caaccagctc tccgacggcg
     1741 acgtcgagcg catgaaggag atgatcaaag cattgaacga gatgctggtg aagcggatcg
     1801 ccggggagga tccgggattc gaggatttca tggcccagta cggggacatg ttcggtgacg
     1861 atccgccgca gtccctggac gagcttctgg agcgcatggc ccagcagatg gcggccgctc
     1921 aatccctgct gaactccatg tcgcccgagc agcagcaaca gctgcagtcc ctgctcagtg
     1981 atcgcttcgg cgatccggag ctgaactctg agcttcgcaa gctcgccaag gagatggact
     2041 tcctcaaccc ggacggcggc aagtaccggt tctccggaga cgagcggctc gatctcgaag
     2101 cggccatgaa gctgatgagc gagatgcagg acctcgatga gctgatctcc caggtccagg
     2161 aagcagagcg cggtgggggc acggaccgta tcgatcggga cctgttgagc gacctgctcg
     2221 gggacgcagc ggccgacgac ctcgagcagc tgcagcagat gctcaaggcc ctggaggaag
     2281 cgggctacat ccgccccacc gacgacgacg gctgggaaat cacgccgagg ggttcccgca
     2341 tgatcggcca gaaggccctt ggcgagatct acgccaggtt gaagcggcag aacctcggca
     2401 atcatgccgt gcccgaggag gggcgtttcg gcgagcggct ggagcagacc aaagcctacg
     2461 agttcggcga cccgttccat ctgcacatgc cccgaaccct gcgcaacgcc atcgatcggg
     2521 agggcccgca gacgccggtg agcctcaagc ccgaggactt cgagatctac cgcagcgagc
     2581 tcatcacgtc cacagccacg gccatgctgg tggacctgtc ctggtccatg gcgctcaggg
     2641 gctctttcca ggcggcaaaa aaggtggcgc tggcgctgca caacctcatc acctcgcaat
     2701 atcccaagga caccttctac atcatcggct ttgccgctta cgccaaggag ctgaagccga
     2761 aggatctacc ctaccttcag tgggacgagt acgtgctggg gaccaacatg cagcacgccc
     2821 tgctgctggc agagcgtctc ctggccaagc accaggcggg cagcaagcag atcatcatga
     2881 tctccgacgg ggagccgacc gcgcacctgg agaacggcca ggcccagttc gc
//
LOCUS       DBNHQR010000514         1725 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_873168, whole genome shotgun sequence.
ACCESSION   DBNHQR010000514 DBNHQR010000000
VERSION     DBNHQR010000514.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_873168"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="AC16VR_11665"
     CDS             complement(<1..733)
                     /locus_tag="AC16VR_11665"
                     /inference="COORDINATES: protein motif:HMM:NF012361.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR6011857.1"
                     /translation="MADPAQSSSSFSFVDMEHQILDFWEQGRVFQRSLENTREKKPYI
                     FYDGPPFATGLPHHGHIVASTIKDIVPRYWTMKGRYVLRRFGWDCHGLPIEHEIDKQL
                     NMSAQQAVAEMGVAGYNQQCRAIVQRYVDEWRHTITRLGRWVDFDNDYKTMDAWYMES
                     VWWVVKQLWEQGLVYQGVKVMPVSTALGTPLSNFEANQNYMDVQDPAITVLFKLVDED
                     AYLSAWTTTPWTLPSNLAICVGPDID"
     gene            837..1700
                     /locus_tag="AC16VR_11670"
     CDS             837..1700
                     /locus_tag="AC16VR_11670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6011858.1"
                     /translation="MEFGAVFPHNEIGTDPGAIKAYAQGVEAMGATHLLIYDHVLGAD
                     PNRPGGWKGAYDKDVAFHEPFTTFAFIAAVTDKLEMITTVLILPQRQTVLVAKQAAEV
                     AILSNNRFRLGVGSGWNQVEYVGLNEDFHTRGRRQAEQVELMRKLWSEDSLTFDGAFH
                     TIDAASINPRPGASVPVWFGGSAAPLLKRCAELGDGWIPLMGANPKARECIDTIRAHR
                     EAAGLPWEGFGIQAQAQYAGGNPDRWASHAGKWQDMGCTHLAIATHNAGPTNADGHLR
                     RIEEYLQAVAA"
BASE COUNT          322 a          549 c          558 g          296 t
ORIGIN      
        1 agtcgatatc gggacccacg cagatggcca ggttggacgg cagcgtccac ggcgtggtcg
       61 tccacgccga caggtaggcg tcctcgtcta cgagcttgaa cagcaccgtg atggccggat
      121 cctgcacgtc catgtagttc tggttggcct cgaagttgga cagcggtgtc ccaagggccg
      181 ttgaaaccgg catcaccttg acgccctggt agacgagccc ctgctcccag agctgcttta
      241 cgacccacca gaccgactcc atgtaccagg cgtccatggt cttgtagtcg ttgtcgaaat
      301 cgacccagcg gccgaggcgc gtgatcgtgt ggcgccactc gtcaacgtag cgctgcacga
      361 tggcgcggca ctgctggttg tagccggcga cccccatctc cgccaccgct tgctgggcag
      421 acatgttgag ctgcttgtcg atctcgtgct cgatgggcag gccgtggcag tcccatccga
      481 agcggcgcag cacgtagcgg cctttcatcg tccagtaccg cggaacgatg tccttgatgg
      541 tgctggccac gatgtgaccg tggtgaggaa gaccggtcgc aaagggcgga ccgtcgtaga
      601 agatgtaggg tttcttctcc cgcgtgttct ccaggctgcg ctggaacaca cggccctgct
      661 cccagaaatc cagaatctgg tgctccatgt ccacgaacga gaacgatgag ctcgattgtg
      721 ctgggtcggc cattggggtc atgcggtcga aaacggccgc gcattgtaac gccattgcgc
      781 gccattccgt agggcactaa gctcgcccgt tcgccgcagg actcgagggg atgtcaatgg
      841 aattcggagc cgtatttccg cacaacgaga tcggaacgga ccccggcgcc atcaaggcct
      901 acgcccaggg agtggaagcc atgggcgcga cccatctgct gatctacgac cacgtgctgg
      961 gcgcggatcc aaaccgtcca ggcggctgga agggggcgta cgacaaggac gtggctttcc
     1021 acgaaccctt caccacgttc gccttcatcg ccgcggtaac cgacaagctc gagatgatca
     1081 ctacggtgct gatcttgccc cagcggcaga cggtgctggt cgcaaagcag gcggccgagg
     1141 tggccattct ctccaacaac cggttccgcc tgggcgtggg tagcggctgg aatcaggtgg
     1201 agtacgtggg gcttaacgaa gacttccata cccggggcag gcgccaggcg gagcaggtcg
     1261 agctcatgcg caagctctgg agcgaggaca gcctgacctt cgacggcgcc tttcacacca
     1321 tcgacgccgc cagcatcaat ccgcgccccg gcgccagcgt cccggtgtgg ttcggcggca
     1381 gcgccgcgcc gctgttgaag cgctgcgccg agctcggcga cggctggata cccctcatgg
     1441 gcgcgaatcc gaaggccagg gaatgcatcg acacgatccg tgctcaccgc gaagccgccg
     1501 ggctgccctg ggagggattc ggtattcagg cccaggccca gtatgcgggc ggcaaccccg
     1561 atcgatgggc atcccatgca ggcaagtggc aggacatggg ctgcacccac ctggccatcg
     1621 ccacccacaa cgcggggccg acgaacgcgg acggtcatct gcgccgcatc gaggaatacc
     1681 ttcaggccgt ggcagcctga cgccgccggg agctggcagg acgat
//
LOCUS       DBNHQR010000515        17511 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1506006, whole genome shotgun sequence.
ACCESSION   DBNHQR010000515 DBNHQR010000000
VERSION     DBNHQR010000515.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 17511)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17511)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17511
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1506006"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..2760)
                     /locus_tag="AC16VR_11675"
     CDS             complement(235..2760)
                     /locus_tag="AC16VR_11675"
                     /inference="COORDINATES: protein motif:HMM:NF027306.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CS1-pili formation C-terminal domain-containing
                     protein"
                     /protein_id="HIR6011859.1"
                     /translation="MFRRPPTWQVAWTLVAATLMLATGAVRATEGGPANVDEQPVVAA
                     VPTRLHPAPIPPARRDTWIPPGFEDLAAPQTTLVDVFYGGDFLISTMVRFSLDEVVIL
                     HPEAVADRLPDLIDPRGFAEKLARAFYPNSELLCRSKYQQDCELIETDDVDLIFDRHR
                     LQLSLFIGSPLLRTRTTEQMKYLPASDAGASIMNDTAAYYSGFVGEDLTYNVASESVF
                     AFAESRISARSNLTDTHGLEVDELAFRREFRGRDLQLGLFRSNIGGFTFLQNQQFIGV
                     NAESSLTTRANVDYALASDVTVFLHSRSRVEIFKDGRLLQSGFYNPGNQVLDTSNLPS
                     GAYDVEIRITDSAGRVTTQPYYFSKTPRLPPTDQALYFFQAGRETVGGANDFFPETAD
                     QGFVRAGLRKRLGASTGGGAALSLNGDSVLVESSLFKQGRNLQAELSAAFESLGIAGV
                     DLGLRYSWRGANLAASARRVWHVEQQPVAQEAMLVDDPAQIGAATTQYSTTFSMPAWK
                     GRFGGFFRYSERDGSALQNYGLNWSASGWSVAGGTLAANIEVSRNDGSVFGAIRLSYR
                     FSREAWSGGVSATLEGNKASDRAFGVDPRGRAEMRWRSDESFYNDYEFALAASSESER
                     DVEAYASVASDMGTAETSVRWIDSTSRTEIRGQLGTRFAATPQSLSFGGRGHAQSAVL
                     VGVDAGEGSDAEFEVLVDTVKRATLRAGQTRLIALGAFRTYDVEVRSVGDTFASLNKT
                     KERTTVYPGNVLSLNWKAKRIFVGMGRLVDKDGEPIRNALIKNASGLATTDEYGFFQA
                     ELDQSAEMLAVQKRGSSCLVDLGEFEVVDQVSYLGTLTCTMSS"
     gene            complement(2783..3469)
                     /locus_tag="AC16VR_11680"
     CDS             complement(2783..3469)
                     /locus_tag="AC16VR_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043711 - pilus organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis chaperone"
                     /protein_id="HIR6011860.1"
                     /translation="MVALATGSACAELVVNRAIVVFDSPSQHRQDVELVNIDAGDNLF
                     VDIESFQVERPGEDDQALTSLRGNENPGLIATPQKAMIGPSNSQLVRFMNLDRDGDRE
                     KVFRVNMTPVTRPPEFDAGDVARGETASQIQIVIGYQVLVIVPPVDPRAVVDYGRSGS
                     SATFKNSGNSNYLLTDGRQCDPDAPEVCMDLVSYRVYPGNHRQVELPFDGPFSYKIRT
                     VAGITSQRFE"
     gene            complement(3552..4127)
                     /locus_tag="AC16VR_11685"
     CDS             complement(3552..4127)
                     /locus_tag="AC16VR_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011861.1"
                     /translation="MLARLRTASVNALCLGIALLVVVGGGSARGAQPNGIDGAFGETS
                     KAEARVTLRIRSGARIVRGPGTGGRPDAPPGGGRGRPDLNLSSRDLSETGEAVLPLCL
                     NAPSGQAFEVSALPDGGDEASGLRTASGSAVSYEVSLGAEGGASATFISSGRDCTPEN
                     SLRLSISTTRSAPDTELTNLTGHVRLFVVPV"
     gene            complement(4133..5077)
                     /locus_tag="AC16VR_11690"
     CDS             complement(4133..5077)
                     /locus_tag="AC16VR_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011862.1"
                     /translation="MTYAANIGNDNYLSVGGVPDVDFGTWSGGSLSTTVRGCIASANE
                     RQPNPPSPPAEVYPYQAGVDNRNPGSDFYLYLNNDASSPESARITVVVEHRDIVAGSA
                     FETLTEGVFDTHYHLGRFQECLGTGDNSELRISIPEANLTNKVAGVYHGQFRLEAVGG
                     TSGTKYDRTDFDLILTVQSANVRISGMDDMALGTYVPGTGNVRQDEHFCVQSLADNAA
                     YRISVSSSNQTPGGDFRLKHATDAVYLPVDVLFSSVGSGSGTEQITNNSISALGSPDS
                     FCGGSDNATITVRALEADVQAVPSGSYGDTLVLLVVPE"
     gene            complement(5263..5784)
                     /locus_tag="AC16VR_11695"
     CDS             complement(5263..5784)
                     /locus_tag="AC16VR_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011863.1"
                     /translation="MKKTSAIALTTLGLLAASSAMAATDGSYDTTSTGTTDVSLTVVD
                     ATRITGLDDIDLGTYAATDTDGVDASDTFCVHVNGGDAYNITATYGPLASPTTSDTIA
                     VQVSLIDNAAGTGAEDVNTGGVSSNYTGAALIDCGGSDNAYINVEVEDADIRAATTAS
                     DYATTVSLLVSPQ"
     gene            complement(6201..8159)
                     /locus_tag="AC16VR_11700"
     CDS             complement(6201..8159)
                     /locus_tag="AC16VR_11700"
                     /inference="COORDINATES: protein motif:HMM:NF012927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR6011864.1"
                     /translation="MKLLSPVEVGPIEVKNRVVSTAHAAFLEFFNPVSDGERYMAYQE
                     RRAQGGTGLIIFTAMHVHESSQIPYHYVFDADTMAPKFAQISTRLHRHGAKCISQLFH
                     FGAQGKSDPRDDFHPLWSFSGTTTLEGEVTHKMTDAEIEEVIDAFAFAAKVATENGMD
                     GVELHGTHGYLIQQSFSPFANQRDDKWGEELVFVKTLARRVRDAIGPGKIMGFRISAD
                     DFIDPADGGVGHERLCHIASEVVGTGLFDYLNHSEGAGGVHYARAIGSFRHKFGEYLP
                     LTRNLRDAIGGAVPLIGVGKIPTPDLAEQALQAGDCDLVGMTRAQIADPDLVSKVAAG
                     RAHRVRTCTGSNQGCFERASYPITCFQNPEVGEENRFKALDIPVVQLKKVLVVGGGPA
                     GMKAAEIAARRGHSVTLAESGDRLGGRLNLVETAGDATNLLSATAWIEEELGGLDVDI
                     RLQTTVDEAFVRQVNPDAIVLATGAYVTNDLGVATDGSVQVLSTDDAASGTFEGNRFE
                     LEGTRCLMIDFRGNYETAVVLESLGRRGSKVTLVTPAPVFGRNIGVSHADDFRRTVIP
                     QLGIRLLPNTTLAQISQGRGHLVNHVTGEEIEEFFDFIVAGTPPRPRDVLFEVLGRHA
                     PVTVVGDAVAPRDAMLAFREGDRAGRTI"
     gene            complement(8275..9810)
                     /locus_tag="AC16VR_11705"
     CDS             complement(8275..9810)
                     /locus_tag="AC16VR_11705"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6011865.1"
                     /translation="MVETVTGTNRSAGVSYSEVLDRDSHPVRDILRVDNPLPPGPTIV
                     DPKVYYSREVFEREKERLWKRVWQMACHEDDIPNVGDAIVYDIADLSYMVVRSAEDEF
                     HAFPNACLHRGRQILHQNEERLHNLRCAFHGWSWGLDGELQEVPCHWDFPTVSKETHS
                     LKQVKVGRWGGFVFINPDPDCEPLEDFLGNLDEHMTLLPMERRYKAVHVAKRLPCNWK
                     VAHEAFAEAYHSVATHPTLLSTLGCANSKYDIFGNFSRALSAHHVGSPHVPSWLAEHP
                     YLEATPFTKQRHELTGSIYERVDDGRVKVTTTDNRVGYFDDRGNHIDGEKFHADIHLC
                     NWVGGKLIEGMDELPSSVITGDFAEKRAKAAAVRREELRKILGDKMDEVSDAELLDPI
                     YYSLFPNVSPWGSFNQIFYRFRPYGDNPEECIHECLFMWPVAEGQPRPPAAKMQWLDL
                     DDDYVDAPELGHLAKVFNQDTLNLPYVQKGLHSLDEVIFANYGETKPRHFHMLYNEWM
                     SKE"
     gene            10062..11690
                     /locus_tag="AC16VR_11710"
     CDS             10062..11690
                     /locus_tag="AC16VR_11710"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR6011866.1"
                     /translation="MKDHDVKPLGRLLTAAAAAHPDRDAMVLVDERATYAELVCRAKR
                     AGRSLMALGVEPGDRVGILMANCLDFVDILFGTTLIGAVPVLYNARFKAREIAHVTAD
                     AGVKVVVTNDIVEQHTNYVELLRRSLPGLAEHDAIRTLALKAAPDLRTAVTLSSSSTA
                     GFLNRNAFYELGSTHPEEVVDERGARVRPEDVAVMIYTSGTTAMPKGCPLTHLVLQHA
                     GVVGGVERLGLIEGDVMWAPLPMFHTAFTQPLTGILHVGGTFLSMTHFDAAAALRMIH
                     DEQATVMFPAFPTITMQLLDHPSYTAQSFACVRVAINVGPPEELCAMQERMPHTTQIT
                     VFGMTETGGSAAMCERDDSLAHRTETSGFALPGNEIQIRDPDSGAELPAGERGEIVVR
                     GRGVFSGYHKAPEKTAESFYDGGWFRTGDLGVMDADGRVTFRGRLKDMLKVGGENVAA
                     VEVEGFLATHPAVNLAQVVAVPDDKYGEVPAAFVELAPGASASEQEIIDYCKDEIASF
                     KIPRFVRFVEEWPMGATKILKYELRERICSELGL"
     gene            complement(11820..12635)
                     /locus_tag="AC16VR_11715"
     CDS             complement(11820..12635)
                     /locus_tag="AC16VR_11715"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6011867.1"
                     /translation="MSERPATEQFGEFTTLERRGRVVVASFDRGDGLNALSSNLMREL
                     TALAGELADDTESSVVVLTGRGAFSAGADLKERDAGASRTPTLLERRQALSIGPDLCA
                     AWERLEQITIAAVEGFCIGGGVALAVACDHRVAGSSAHFRLPEVPLGMNMSWQSNPRT
                     VALIGPSRAKLFTILGEKLAADTALEWGLVDEVVADGQALASAVSLADRYAAMPPLPL
                     RMTKQAINAATGALNYATSYMDRDQFLLTALSDDQKEGVRAFLEKRTPEFRGD"
     gene            complement(12863..13693)
                     /locus_tag="AC16VR_11720"
     CDS             complement(12863..13693)
                     /locus_tag="AC16VR_11720"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR6011868.1"
                     /translation="MRKFLGRMTRRGIVSLLAGLLVTLPIGVNAAKDQSPVLAQIAKW
                     GKIRVGMSGSQPPYNFVSREGKLIGMDVDIAELLAKSLDVQLEMVQMNFNELLPALES
                     GKVDLVISGMTATLQRNMRAAFVGPYHITGKSILTRSETIAALGSNDLNREDLRIAAL
                     KGSTSEDFVRRVAPNAKLVATDTYDEAIDQLMANKAELFVADAPRVMLEVMRWPDAGL
                     TAAQRPLTIEPIGIAVPASDPLLLNLVENYMAALESSGALEKLEEKWFKSGGWLVQLP
                     "
     gene            complement(13814..14830)
                     /locus_tag="AC16VR_11725"
     CDS             complement(13814..14830)
                     /locus_tag="AC16VR_11725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010221163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HIR6011869.1"
                     /translation="MQAIRCHEISEDIGAVRLETISLDPPGEGEVQVRLKACAVNFPD
                     LLMIQGKYQFKPPLPFSPGTEAAGDVVAVGDGVSAFDVGDRVVVALRYGGFAEAVNVP
                     AESVQPVPGSMSYDKAASYRTAYLTAYVALAKRGDLTPGEVLLVHGATGGVGMAAVDL
                     GKRLGAVVIATGGSDEKLAVVKARGADHVINYTQADGTLGGFREEVKALTDGRGADVI
                     YDPVGGDVFDESMRCINWGGRILTIGFTSGRWPQAPDNLILIKQIAVIGVRAGESGRR
                     DPATGREAERALYAMAAAGEIDPYVCAGFPLERAVEAMRMLEQRRVVGKVVVTMNGFD
                     VASP"
     gene            complement(14840..15235)
                     /locus_tag="AC16VR_11730"
     CDS             complement(14840..15235)
                     /locus_tag="AC16VR_11730"
                     /inference="COORDINATES: protein motif:HMM:NF014790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6011870.1"
                     /translation="MIRDAETFEHLLAAVRRFVAEVAVPNEERVEADDEIPAEGVEAM
                     RNLGAFGRSIPEAYGGSGLTSEELALAFMELTRCSVAYRVVQIFGGQGYVKGRGSERF
                     YRDVRAFRIYEGTSQIHLLNIAKQLLKDA"
     gene            complement(15235..16179)
                     /locus_tag="AC16VR_11735"
     CDS             complement(15235..16179)
                     /locus_tag="AC16VR_11735"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024809.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6011871.1"
                     /translation="MEIYLHHYPASLFSEKIRLLLGYLGGSWHSVEIPSIMPRPLLMP
                     LSGGYRRTPVLQIGANVYCDTAVIARGLARHFGDEDLFAAGFTANRVAEWADTQLFRV
                     TVALNFRPEAVGGFMSQLSPEEIAAFQKDRAELVGDAPMVSFSPEAAQAYLATYLDQA
                     DRALDGGFLFGGKPCIADFCFYHCLWFLENNPLNAPLLDAYGNVRGWMQTMAGFGHGS
                     VAPSSGEAALEHARSCSPVAPVLESPLAAPFELDVGRCVTVTPADYGRVPVAGSLVAW
                     DCEEVVILREDAATGPIMNHFPMAGFQVAPAGNLGSSG"
     gene            complement(16264..>17511)
                     /locus_tag="AC16VR_11740"
     CDS             complement(16264..>17511)
                     /locus_tag="AC16VR_11740"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF039907.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HIR6011872.1"
                     /translation="EVAIRTGSNFSASIREGELETLVEAGFRELEVKLIVDQKVATEA
                     SSDLSEATLKLLIDNGIGRARLSGADPFAGLPEKEPVKVRAEALDIFDEAVVDMSPES
                     KIAAARRVEAICLGDKRIRRSMGAGFGTTIETLYLANSNGFSDAFRQTACSNGVYLQV
                     GEPDNLISEGKYDYSRNLEGLRSPEDIAAEAIDRSTRLIGARKVPTQNVPVVLEPEMS
                     AELLQFLASCVDGRGVYRKQSFLADRIGERVAGANVNVFDDGLMPGAPGAKPFDREGV
                     PLRKTAVIEKGVLKSFLLDSYSARKLGMKPTGNGAGPNNLYLASGKDSPADIIGSVDE
                     GLLLTGSIGFGAVPTTGDISRGAFGIWIEGGELAYPVAGITIGANLGTLLQQVEMIGD
                     DLDHRRYVNGPTIKVAEMTIGGT"
BASE COUNT         3230 a         5678 c         5478 g         3125 t
ORIGIN      
        1 gaaagttcgt tgaaattcgc ggatctcgta gtccatgatt tagtcgtgaa tcccagcaaa
       61 tacgcggctt tcgcgatagt cgggcacctg cctgaaaatc cgcgtgtcgg cagttcgatt
      121 ctgcccctgg ccaccagacg agacctaagg tcgaggcgca gtggccagcg gccaccagac
      181 gagacctaag gtcgaggtgc agtggccagc ggccaccaga cgaggcagaa gctgttagga
      241 cgacatcgtg caggtcaggg taccgagata ggaaacctga tccacgacct cgaactcgcc
      301 gaggtcgacc aagcagctgg aacccctttt ctgaactgcc agcatctcgg cgctttggtc
      361 gagctccgcc tgaaaaaagc cgtattcgtc cgtggtcgcc aggcccgatg cgttcttgat
      421 cagcgcgttg cggatcggct cgccgtcttt gtcgaccagt cggcccatgc cgacgaagat
      481 gcgcttcgcc ttccagttga gcgacagcac gttgcccgga tacacggtcg tgcgttcttt
      541 tgtcttgttg agggaagcaa aggtatcccc caccgaccgc acctcgacgt cgtaggttct
      601 gaaggcgccg agcgcaatca gccgggtctg gccggcccga agcgtggcgc gcttgaccgt
      661 atcgaccagc acttcgaact ccgcgtcgga cccttccccc gcatcgaccc ccacaagcac
      721 cgcgctctgc gcatggccac ggccgccaaa gctcagtgat tgtggggttg cagcgaaccg
      781 ggtgcccagc tggccgcgaa tctcggtgcg gctcgtcgag tcgatccagc gcaccgacgt
      841 ctccgccgtg cccatgtcgg aggcaacgct tgcgtaggct tcgacgtcgc gctcggactc
      901 ggacgaagcg gccagcgcga actcgtagtc gttgtaaaag gactcgtcac tgcgccagcg
      961 catttccgcc cgaccgcgcg ggtccacgcc gaatgccctg tccgaagcct tgttaccctc
     1021 gagagtagcc gagacgccac cggaccacgc ttccctggag aatcgatagc tcaatcgaat
     1081 cgcgccgaac accgagccat cgttccggga aacttcgatg ttggcggcaa gggtgcctcc
     1141 ggcgacactc caaccgctgg ccgaccagtt cagcccgtag ttctgcaggg cagagccatc
     1201 gcgctcggag tatcggaaaa agccgccgaa gcgccctttc caggccggca tagaaaacgt
     1261 ggtgctgtac tgggtcgtcg cggcgccgat ctgggcgggg tcatcgacaa gcatggcttc
     1321 ctgagctacc ggttgctgct cgacgtgcca gacacgccgg gcagaagcgg cgagattggc
     1381 gccccgccag ctgtatctca ggccgaggtc cacccccgca atgcccaggc tttcgaatgc
     1441 cgcactgagc tccgcctgca gattgcgtcc ctgcttgaag agactggact cgacgagcac
     1501 gctgtcgcca ttcagggaaa gcgccgcgcc gccaccggtg gacgctccca gtcgcttgcg
     1561 cagcccggca cggacaaacc cctgatccgc ggtttccgga aaaaaatcat tggctccccc
     1621 cacggtttcg cgcccggcct gaaagaagta caacgcctga tccgtgggcg gaagccgcgg
     1681 cgtcttggaa aagtagtagg gctgcgtggt gacccggcca gccgagtcgg tgatgcgtat
     1741 ctccacgtcg tacgcaccgg acggcaggtt cgaggtatcc aggacctgat tgccggggtt
     1801 gtaaaaaccg ctctgcagca gccggccgtc cttgaagatc tccacccggc tgcggctgtg
     1861 caggaagacg gtgacgtcgc tcgcgagggc gtagtccaca ttggctcggg tggtgaggga
     1921 gctctccgcg ttcacgccga tgaactgctg attctgcaga aaggtgaaac cgccgatgtt
     1981 cgaccgaaac aatccgagct gaaggtccct gccgcgaaac tcacgacgaa acgcaagctc
     2041 gtccacctcc agaccatgag tatccgtcag gttgctgcga gccgagatcc gactctcggc
     2101 aaaagcgaag acgctctcac tggcgacgtt gtaggtcagg tcttcgccaa cgaacccgct
     2161 gtagtaggcg gccgtgtcat tcatgataga ggcccctgca tcggacgcgg gcaggtattt
     2221 catctgctcc gttgtgcgcg tccgaagcag cggcgaaccg atgaagaggc tcaactgaag
     2281 gcggtggcga tcgaaaatca ggtcgacgtc gtcggtttcg atgagttcgc agtcctgctg
     2341 atacttggat ctgcacagca gctcggaatt cggatagaag gcccgagcga gcttttccgc
     2401 aaatccccgt gggtcgatca ggtccggcag gcggtcggca accgcctccg gatgcaggat
     2461 aacgacctcg tcgaggctga agcggaccat ggtggaaatg aggaaatctc cgccgtagaa
     2521 gacgtccacc aaagtggtct gcggcgcagc aaggtcttcg aaacccgggg gaatccaggt
     2581 atcccgacga gccgggggaa tcggcgccgg atgcagccgg gtgggcacgg cggcgaccac
     2641 cggctgctcg tcgacgtttg cagggccgcc ctccgtcgcc cggacggcgc cggtcgcgag
     2701 catcagggtt gccgcgacca gggtccaggc tacttgccac gtcggcggcc tgcgaaacat
     2761 ctagcgagcg aaggccgtgc aactactcga agcgctggct ggtgatgccc gccaccgtcc
     2821 gaatcttgta gctgaagggc ccgtcgaagg ggagctcgac ctgcctgtgg ttgcccggat
     2881 aaacccgata ggaaacaagg tccatgcaaa cctccggcgc gtcaggatca cactgacgac
     2941 cgtcggtgag aaggtagttg gagtttccgg aattcttgaa ggtcgcggag cttcccgagc
     3001 ggccgtagtc aaccactgcc cggggatcta ccggcggaac gatcaccaag acctgataac
     3061 caatcacgat ctggatctgc gaggcggttt cgcctcgtgc aacatctccc gcatcgaatt
     3121 ccggcggcct ggtcacgggc gtcatgttca cccgaaatac tttttcccga tcgccgtcgc
     3181 gatccaggtt catgaagcgc acgagctggc tgttggaggg gccgatcatg gccttctgcg
     3241 gcgtcgcgat cagcccggga ttttcattgc cccgcaacga ggtcagggcc tggtcgtcct
     3301 ctcccggccg ctcgacctga aaggattcga tgtcgacgaa aaggttatca ccggcgtcga
     3361 tgttgaccag ttccacatcc tggcgatgct ggctgggaga gtcgaagaca acaatggcgc
     3421 gattgaccac cagctccgcg cacgcggagc cggtggccaa tgcgaccatg gagcaaacga
     3481 tgatcgaaca gatatggaaa cgctcagttt tcacggcagc ctcctatttg gcgtctggtt
     3541 gatgtgcgtg gtcacacggg aacgacgaac agccggacgt ggccggtcag gtttgtgagt
     3601 tcggtgtccg gcgcagatcg ggtggtgctg atcgacaggc gaagagagtt ctccggcgtg
     3661 cagtcgcgac cgctggatat gaaggtcgcc gacgcgcctc cttcggcccc aaggctaacc
     3721 tcgtaactca cggcggagcc cgatgccgtg cgcagcccgg aggcttcgtc accgccgtct
     3781 ggcaacgcgg acacttcaaa ggcctgcccg ctgggcgcgt tgagacacag cggcagcacc
     3841 gcctcacccg tctcgctgag atcccggctc gacagattca ggtcgggtct ccctcgcccg
     3901 ccgccgggag gcgcgtccgg gcgcccgccg gtaccgggtc ccctgacaat ccgcgcaccg
     3961 ctccggattc gcaacgtcac gcgcgcttct gctttgctgg tctcgccaaa cgccccatcg
     4021 atcccgttcg gctgggcgcc ccgggcgctt ccgcccccga cgacgaccag caaggcgatg
     4081 ccaaggcaga gcgcattgac cgaagcagtt cgcaaacgtg ccaacatcgc cgctactccg
     4141 gtacgaccag cagaaccagc gtgtccccat aggagcctga ggggacggcc tgcacgtcgg
     4201 cctctaaggc gcgcacggtg atggtggcgt tgtcggagcc gccgcagaag ctgtcggggc
     4261 tgcccagggc cgagatggag ttgttggtga tctgctccgt gccgctgcca gaccctacag
     4321 aagagaagag cacgtccaca ggcaggtaaa cggcatccgt ggcgtgcttc agtcgaaaat
     4381 cgcccccagg ggtctgattg ctggatgaga cgctgatgcg gtaggcagcg ttgtccgcga
     4441 ggctctgcac gcagaaatgc tcgtcctgcc gaacgttacc ggtcccgggg acgtaagtac
     4501 ccagcgccat gtcatccata ccgctgatac ggacgtttgc gctctgcacg gtgaggatca
     4561 gatcgaaatc ggtccggtcg tacttcgtcc cgctcgttcc ccccaccgct tcgagccgga
     4621 actgcccgtg atagaccccg gccaccttgt tcgtcaggtt cgcctccgga atggagatgc
     4681 gcagttccga gttgtccccg gtcccaaggc attcctggaa gcggccgaga tggtagtgcg
     4741 tgtcaaagac cccctcagtg agggtctcga acgcgctccc cgcaacgatg tcgcggtgtt
     4801 cgaccacaac ggtgatgcgg gcactttcag gactcgaggc gtcgttgttc agataaagat
     4861 agaagtccga gcctggattg cggttgtcca caccggcctg gtacgggtaa acctccgccg
     4921 gcgggctcgg cgggttgggc tggcgctcat tggcagaagc aatgcagccg cgcacggtgg
     4981 tggacaggct tccgccggac caggtgccga agtccacgtc ggggacgccg ccaacgctta
     5041 agtagttgtc gttaccaatg tttgcggcat aggtcatctt aggaaacccg gcggacagca
     5101 ggacgcaaag cccggtgagc aggagcggaa gttgcggcca tctgtgtaga ggaatggggt
     5161 gcaagtcgga tcagctcggt gagtgaaaac atgtcggtca gctcggaaac cgggcgccgc
     5221 gggcctggtg cccgtgttcg ggcaccaggt caccgccccg ggtcactgag gcgaaaccag
     5281 cagcgagacg gtcgtggcgt aatcgctggc cgtcgtcgcg gcgcggatat ccgcatcctc
     5341 cacctcgacg ttgatgtacg cgttgtcact gccgccgcag tcgatcaggg cagctcccgt
     5401 gtagttggaa gagacacctc cggtgttgac gtcttcggcg cccgtgccgg cagcgttgtc
     5461 aatgagcgac acctgcacgg cgatcgtgtc cgacgtggtc gggctcgcca ggggtccgta
     5521 ggttgccgtg atgttgtatg cgtcaccgcc gttgacgtgc acgcagaacg tatccgaagc
     5581 gtccacgccg tccgtgtcgg tggctgcgta ggtgccgagg tcgatgtcgt ccagcccggt
     5641 gatgcgggtg gcatccacga ccgtcaggct gacgtcggtg gtgccggtgc tggtcgtgtc
     5701 gtaggacccg tcggtggccg ccatggcgga gcttgcggcg aggaggccga gggtagtcag
     5761 ggcaattgcg gaggtctttt tcactgttca gattccattg gtagttgagt cacacgttta
     5821 cgacgacctt caggtgaagg atctgccgcg cgcttcaaca gccgttgctg agttccgatc
     5881 tgcctgagct gggatcccgg catactgcat cgaagcttcg agcccctggt gcagggggcg
     5941 tgccaacttt ccggcaatgc aattttgctg gccgcgtcgc cgcctgaaat tcttatgaat
     6001 ttcagcttgt tagcgacgag cgaagaaatt ttcccgacga gcggccgctg ctcgccgtag
     6061 ccgacatgga tcgcgcgcga ttgtcacagc gggtcacatt tcgacccacg ctgtgacgcc
     6121 ttcgtcagtc gagccgagaa gccaggaaca cgatttgtgc ccagcgcgtc atggcggcag
     6181 agagatcggg cacgcggggg tcagatggtg cggcccgccc ggtccccctc gcggaacgcc
     6241 agcatcgcat cccgcggcgc gacggcatcg cccaccactg tcaccggggc atggcgaccg
     6301 agaacctcga agaggacgtc ccggggccgg ggcggcgtgc cggcaacgat gaagtcgaag
     6361 aactcttcga tctcctcacc cgtcacgtga ttgaccagat ggcccctgcc ctggctgatc
     6421 tgcgccagcg tcgtgttggg cagcaagcgg attcccagct gcggaatgac cgtgcgtctg
     6481 aagtcgtcgg cgtgggagac gccgatgttg cggccgaaga ccggcgccgg ggtcaccagg
     6541 gtaaccttgc tgccccggcg tccgagcgat tcgagcacca cggccgtttc gtaattgccg
     6601 cggaaatcga tcatcaaaca gcgcgtccct tccagctcga agcggttgcc ctcgaaggtg
     6661 ccggaggctg cgtcatcggt ggacagcacc tgcacggaac cgtcggttgc gacgccgagg
     6721 tcattggtca cgtaggcgcc ggtggcgagg acgatggcgt ccggattcac ctggcggacg
     6781 aatgcttcgt ctacggtggt ctgcagcctg atatcgacat ccaggccgcc aagctcctct
     6841 tcgatccatg cggttgcgct caacaggttg gtggcgtcgc cggccgtttc caccaggttg
     6901 agccgtccgc caagccggtc accagactcg gccagggtga ccgagtgtcc gcgcctggcg
     6961 gctatctcag ccgccttcat gcccgccgga ccaccgccca ccaccagcac cttcttgagc
     7021 tggaccacgg ggatgtcgag ggctttgaag cggttttcct caccaacctc ggggttctgg
     7081 aaacaggtga ttggatacga ggcgcgctcg aagcagccct ggttggagcc ggtgcaggtg
     7141 cgcacgcgat gggctcggcc cgcggccacc ttgctgacca gatcgggatc cgcaatttgc
     7201 gcgcgggtca tgccgacgag atcgcagtcg ccggcctgga gcgcctgctc cgccagatcc
     7261 ggggtcggaa tcttccccac accgatgagg ggcaccgcac ccccaatcgc atcgcgcagg
     7321 ttgcgcgtca gcggcaggta ctcgccgaac ttgtgccgga acgagccgat ggcgcgcgca
     7381 tagtgcacgc cgccggcacc ctcggaatgg ttcaggtagt cgaagaggcc cgtgcccacc
     7441 acctcggagg cgatgtggca tagccgctca tggcccacgc cgccgtcggc cggatcgatg
     7501 aagtcgtcgg ccgagattcg aaatcccatg atctttccag gcccgatggc atcccgaacc
     7561 cggcgcgcca gggtcttcac gaaaacgagc tcctcacccc acttgtcgtc gcgctggttg
     7621 gcaaaggggc tgaaagactg ctggatcaga tagccgtggg tgccgtgcag ctccacgccg
     7681 tccatgccgt tctcggtagc gaccttggcg gcgaaggcga aagcatcgat gacctcctcg
     7741 atctccgcgt cggtcatctt gtgggtgacc tcgccttcca gcgtcgtggt tccggagaag
     7801 ctccacaaag gatgaaagtc atcgcgaggg tccgacttgc cctgagcacc gaaatgaaac
     7861 agctgggaga tgcatttcgc cccgtgccga tgcagccgcg tcgagatctg cgcgaacttc
     7921 ggcgccatgg tgtccgcatc gaaaacgtag tggtagggga tctggctcga ttcgtgtacg
     7981 tgcatggccg tgaagatgat cagcccggtc ccgccctggg cacgacgctc ctgatacgcc
     8041 atgtagcgtt cgccatcgga gacgggattg aagaactcga ggaacgcagc gtgggcggtg
     8101 gagaccaccc ggttcttcac ctcgatcggg ccaacctcga cagggctgag cagcttcatc
     8161 atggcgttcc tcgcagaccg atacttcgag tgtagtaccg ccacagagca aatgtctcga
     8221 ttcgttcgac aggcatcggc ccccgctcgg ctcagcgaat tccgcccgcc ttgcttactc
     8281 cttcgacatc cactcgttgt agagcatgtg gaagtggcgc ggtttggttt ctccgtaatt
     8341 ggcgaagatc acctcgtcca gcgaatgcag ccccttctgc acgtagggca ggttcagggt
     8401 gtcctggttg aagactttcg cgagatgtcc gagctccggc gcgtcgacat agtcatcgtc
     8461 cagatccagc cactgcatct tcgccgccgg cggacgcggt tgtccttcgg ctaccggcca
     8521 catgaacaga cactcgtgga tgcactcctc cgggttgtcg ccgtaaggtc ggaaacggta
     8581 gaagatctgg ttgaaagacc cccaggggct gacgttgggg aacaggctgt agtagatggg
     8641 atcgagcagc tccgcgtcgc tcacctcgtc catcttgtcg ccgagaatct tgcgcagctc
     8701 ttcgcgccgc acggcagccg ccttcgcacg cttttcggca aagtcgcccg tgattaccga
     8761 agagggcagc tcgtccatcc cctcgatcag cttcccgccc acccagttgc acaagtgaat
     8821 gtcggcgtgg aacttctcac catcgatgtg gttgccacga tcatcgaagt agcccacgcg
     8881 gttgtcggtg gtggtgacct tgacgcgccc gtcatcgact cgttcgtaaa tgctgccggt
     8941 gagctcgtga cgctgcttcg tgaacggcgt cgcctccaga taggggtgct ccgccagcca
     9001 cgatggcacg tgggggctgc caacatgatg ggccgacagg gcgcgggaaa agttcccgaa
     9061 gatgtcgtac tttgagttgg cgcaccccag cgtgctgagc agcgtcgggt gggtcgctac
     9121 cgagtgatag gcttcggcga acgcctcgtg ggcgaccttc cagttgcacg gcagccgctt
     9181 ggcgacgtgc acggctttgt accgtctttc catgggcagc agcgtcatgt gctcgtccag
     9241 attgccgagg aagtcctcca gcggttcgca atccggatca ggattgatga acacgaaccc
     9301 gccccagcgg ccgaccttca cctgcttcag ggagtgggtc tccttcgaga cggtggggaa
     9361 atcccagtga cagggcacct cctgcagctc gccgtcgagc ccccaggacc agccgtggaa
     9421 agcgcagcga agattgtgca gacgctcttc gttctggtgc aggatctgcc ggccgcggtg
     9481 caggcaggca ttcgggaacg catggaactc gtcctctgcg ctgcgcacga ccatgtagga
     9541 aaggtccgca atgtcgtaga cgatggcgtc gccgacgttg ggaatatcat cctcgtggca
     9601 cgccatctgc cagacccgct tccacaagcg ctctttctcc cgttcgaaaa cctcacggga
     9661 gtaatagact ttcgggtcga cgatcgtcgg gcccggcggc aacgggttgt ctacacgcag
     9721 gatgtcccgc accgggtgag agtcgcggtc gagcacctcg ctgtaactga cgcctgcgga
     9781 tcggttcgtg cccgttactg tttcgaccat gaattcgccc cctccaagca gatcaaatgc
     9841 gcctcatcta tcagcgcatc tatcagaccg gggggcaata gcggaaactg cgtacggcca
     9901 accccgggag gagctggtaa cgctagcaaa agggcgtcat ttcacgggcg gctgcatgcg
     9961 cgtcgccgct caagccctta aactatcgtc cggttatgct cagcggaaat atccgaagtg
    10021 cctgtgcttt ctcaccacgc cgacgagaat tcgccttgcc catgaaagac cacgacgtca
    10081 aacctctggg ccggctattg accgccgccg cggccgcaca ccccgaccgc gatgccatgg
    10141 tgctcgtgga cgaacgcgcc acctacgcag agctcgtctg ccgggcaaag cgcgccggcc
    10201 gctcgctcat ggcgctgggc gtggaacccg gcgaccgggt gggcatcctg atggccaact
    10261 gtctggactt cgtggacatc ctgtttggca cgacgctcat cggcgccgtg cccgtgctct
    10321 acaacgcccg cttcaaggcc cgggagattg cgcacgtcac cgccgacgcc ggcgtcaagg
    10381 tggtggtcac caacgacatc gtcgagcagc acaccaacta cgtggagctg ctccggcgct
    10441 ccctgccggg tctcgccgag catgacgcca tccgcacgct ggccctgaaa gcggcgcccg
    10501 atctgcgcac ggcggtgacc ctctcgtcat cgagcacggc cggcttcctg aaccgaaacg
    10561 cgttctacga gctgggctcg acgcatccgg aggaggtcgt cgacgagcgc ggcgcccggg
    10621 tccgcccgga ggacgtcgcc gtgatgatct acacctcggg aaccaccgcc atgccgaaag
    10681 gctgccctct cacccacctc gtgctgcagc acgctggcgt ggtcggcggc gtggagcggc
    10741 tcgggctcat cgaaggggat gtgatgtggg caccgctgcc gatgtttcac acggcgttta
    10801 cccaaccact gacgggcatt ctgcacgtgg gcggcacctt cctctccatg acgcacttcg
    10861 acgccgcggc ggctttgcgc atgattcacg atgagcaggc gacggtcatg ttcccggcgt
    10921 ttccgaccat caccatgcag ctgctcgacc acccttcgta caccgcgcaa agctttgcct
    10981 gcgtacgcgt cgcgatcaac gtagggcctc ccgaggagct ttgcgccatg caggaacgca
    11041 tgccgcacac cacgcagatc accgtattcg gcatgaccga gacgggcggg tcggcggcga
    11101 tgtgcgagcg cgacgacagc ctggcccatc gcacggaaac ctccgggttc gcgctgccgg
    11161 gtaacgagat tcagatccgc gatccggaca gcggcgccga gttgcctgcc ggcgagcggg
    11221 gagagatcgt cgtcagggga cgcggcgtgt tctccggcta ccacaaagcg ccggagaaga
    11281 ccgcagaatc attctacgac ggcggctggt tcagaaccgg cgacctcgga gtcatggacg
    11341 ccgacggccg ggtgacgttt cggggccggc tcaaggacat gctcaaggtc ggcggcgaaa
    11401 acgtcgccgc ggtggaggtg gaggggttcc tggccaccca tcccgccgtc aatctggcac
    11461 aggtagtcgc cgttcccgac gacaagtacg gtgaggtacc agccgccttc gtcgagctcg
    11521 cgcccggagc ctcagcgagc gagcaggaga tcatcgacta ctgcaaagac gagatcgcaa
    11581 gcttcaagat tccccgattc gtccgcttcg tggaggagtg gcccatgggc gcgaccaaga
    11641 tcctgaaata cgagctgcgc gagcgcatct gctcggagct cgggctctga cgagcgtcga
    11701 gcgggtcccc gtcgagggcg gtctgtacgg agcttccccc caaagacaac gctgaggcgc
    11761 ggccaggcag cgcccggttg cctggacagg gctttgaacc cgccctgccc gcgcctggat
    11821 cagtctcctc taaattccgg cgttcgtttc tccagaaagg ctcgcacgcc ctccttctgg
    11881 tcatcgctca gcgcggtgag cagaaactgg tcccgatcca tgtagctcgt tgcgtagttg
    11941 agggccccgg tggcggcgtt gatggcctgc ttggtcattc gcaggggcag cgggggcatg
    12001 gccgcgtacc gatcggccag cgaaacggct gacgccagcg cctgcccgtc ggcgaccact
    12061 tcgtccacga ggccccattc cagcgcggtg tctgcggcga gcttctctcc gaggatggtg
    12121 aaaagcttgg cgcgggaggg accgatcaga gccaccgtgc ggggattgct ctgccagctc
    12181 atgttcatcc ccagcggaac ttccggcagc cggaagtggg cagaggagcc ggccacccga
    12241 tggtcgcacg caacagcgag ggcaacgcca cccccgatgc agaagccttc caccgcagca
    12301 atggtgatct gctcgagccg ctcccaggcg gcacagaggt ccggcccgat cgaaagagcc
    12361 tgacgccgct cgagcaacgt cggcgtccgg gaagcgcccg cgtcgcgttc cttcagatcg
    12421 gcacccgccg aaaacgcgcc ccgaccggtc aggacgacaa cggagctttc cgtgtcgtcg
    12481 gcaagctcac cggcaagggc cgtgagttcg cgcatcaggt tggaggaaag ggcattcaag
    12541 ccatcgccgc ggtcgaacga agcgacgacg acgcggcccc gacgttccag cgtggtgaac
    12601 tcgccgaatt gctcggtggc gggccgttcg ctcatgttcg cctccctggc tggtcctgcc
    12661 cgatcttaac aaaagccgcc cgagccgagc cccggctccg cggtttcagc cgtcttcgct
    12721 ttgggtcatt gacatcccga cgagcgcaag gatgacgggc acaacgagga tcaggttggg
    12781 ctgacggggc gaggataggg acatggtcca ggctcgcacc gaaggaatcg gcgcgaaacc
    12841 aggaccacgg gttcatctca ggtcacggca gctgaacgag ccaaccgccg ctcttgaacc
    12901 acttttcttc gagcttctcc aaggcgccgc tcgactcgag agcggccatg tagttctcca
    12961 ccaggttcag cagcaacggg tcgctggcgg gaaccgcgat gccgatgggc tcgatggtca
    13021 gcggacgctg ggccgcggtc aggcccgcat ccggccaccg catgacctcg agcatgactc
    13081 ggggagcatc ggcgacgaac agctccgcct tgttggccat cagctggtcg atggcctcgt
    13141 cgtaagtgtc cgtggccacc aacttggcat tcggagccac ccgccgcacg aaatcctcgc
    13201 tggtcgagcc tttcagcgcg gcgatgcgca ggtcctcgcg gttcagatcg tttgagccca
    13261 gggcggcaat ggtttccgag cgcgtcagaa tggacttgcc ggtgatgtgg taaggcccga
    13321 caaacgcggc gcgcatgttg cgctgcaacg tggccgtcat accggatatc accagatcga
    13381 ccttgccgga ctccaatgcc ggcagaagct cgttgaagtt catctgcacc atctcgagct
    13441 gaacatcgag cgacttggcc agcagctcgg cgatgtccac gtccatgccg atgagcttgc
    13501 cctcccggct gacgaagttg tacggcggct ggctaccgga catgccgacg cggatcttgc
    13561 cccacttggc gatctgagcg agaacgggcg actgatcctt ggcggcattg acgccgatgg
    13621 ggagagtgac caggaggccg gcgagcagac tgacaatgcc ccttctggtc atacgtccga
    13681 gaaacttacg catgggtctt ccttagttga ttggtttcca tatttcttga cgcccgcctt
    13741 caaacgggcg ggtcgaacgg ggaggccgtt atacaacaag ccaggcgcga cgaacagcct
    13801 ctcggcggag gagctatggt gaggcgacat caaagccgtt catggtgacc accaccttgc
    13861 caaccaccct gcgctgctcg agcattcgca tggcctctac cgcccgctcc agcggaaaac
    13921 ccgcgcagac gtacgggtcg atctcgccgg cggccgccat ggcatacagt gcccgctcgg
    13981 cctcgcgccc cgtcgcggga tcccgccgcc cggactcgcc ggcgcgaacg ccgatcacgg
    14041 caatctgctt gatcagaatc agattatcgg gcgcctgggg ccagcgcccg ctggtgaacc
    14101 cgatggtaag gatgcggccg ccccaattga tgcagcgcat ggattcatcg aacacgtcgc
    14161 cgccaacggg atcgtagatg acgtcagccc cccgcccgtc ggtcagcgcc tttacttcct
    14221 cgcgaaaccc gcccagcgtc ccatcggcct gggtgtagtt gatcacatga tccgcgccac
    14281 gggccttgac caccgccagc ttctcgtcgc tcccgccagt tgcgatgacc acggcaccca
    14341 gacgtttgcc cagatccacc gccgccatgc ccacacctcc ggtggcaccg tgaacgagca
    14401 gcacctcgcc gggcgtcagg tcgccacgct tggccagcgc cacgtaggcg gtgaggtagg
    14461 ccgtccggta cgaagccgcc ttgtcgtagc tcatcgagcc gggaacgggc tgcacactct
    14521 ccgcgggcac attcaccgcc tccgcgaaac cgccgtagcg cagcgccacg accacccggt
    14581 cgcccacgtc gaacgcgctc accccatcgc cgacggcgac gacgtcaccg gcggcttccg
    14641 tgccgggaga aaacggcagg ggcggcttga actgatactt tccctggatc atgagcagat
    14701 cgggaaagtt gaccgcacag gccttcaggc gcacctgaac ctccccctca cccggcgggt
    14761 cgaggctgat ggtttcgaga cggacagcgc cgatgtcctc ggagatctcg tgacagcgga
    14821 tggcttgaat atcggtcacc taggcgtcct tgagcagttg cttggcaatg ttgagcagat
    14881 gaatctggct ggtaccctcg tagatccgaa acgcgcgaac gtcccgatag aaccgctcgc
    14941 tgccgcggcc cttgacgtag ccctgcccac cgaagatctg caccacccgg taggctaccg
    15001 agcaacgggt cagctccatg aaggcgagcg caagctcctc gctggtgaga ccgctgccgc
    15061 cataggcctc agggatgctc cggccgaagg caccgagatt acgcatcgcc tccacgccct
    15121 ccgccgggat ctcatcgtcc gcctccacac gctcctcgtt gggaacggca acctccgcga
    15181 cgaatcggcg cacggctgcc agcagatgct cgaaggtttc tgcgtccctg atcatcagcc
    15241 cgacgacccc agattgccgg cgggagccac ctgaaagcct gccatcggaa agtggttcat
    15301 gatcggcccg gtagccgcgt cctctcgcaa gatcacgacc tcctcgcaat cccaggcaac
    15361 gagggatccg gccacgggca ctcgtccgta gtcggccggc gtcacggtga cacaccgacc
    15421 cacatcgagt tcgaagggag ccgcaagcgg gctctccagt accggggcga cgggactgca
    15481 ggaccgcgcg tgctcgagcg cagcctcgcc gctgcttggt gcaacggatc cgtgaccgaa
    15541 gcccgccatg gtctgcatcc agccgcgaac gttcccatag gcgtccagca gaggagcgtt
    15601 cagcggattg ttttccagaa accacagaca atgataaaag cagaagtcgg cgatgcaggg
    15661 cttcccgccg aacaggaacc cgccgtcgag cgcccggtct gcctggtcga ggtaggtggc
    15721 cagataggcc tgcgccgctt caggggagaa cgagaccatc ggcgcatcac ccacgagctc
    15781 ggcccggtcc ttctgaaagg ctgcaatctc ttccggggaa agctggctca tgaaaccgcc
    15841 gaccgcctcc ggccggaagt tgagcgcgac cgtcacgcgg aacagttggg tatccgccca
    15901 ctccgcgacc cggttggccg taaagccggc cgcaaacaga tcttcgtcgc cgaaatgccg
    15961 ggcgagcccc ctggcaatga ccgcggtatc gcagtagacg ttggcaccga tctgcagcac
    16021 gggcgtgcgt cgatagcctc ccgacagcgg catcagcaaa gggcgcggca tgatgctcgg
    16081 tatctcgact gagtgccagg aaccgcccag gtaccccaga agcagccgga tcttctccga
    16141 aaacagggaa gccggatagt ggtgcaggta aatttccaac tgttccccca gaacggacct
    16201 tcgcggtacg cgcgcgacca ctgcgtttgc cgcccgggac acacgcgtgc tccgtacccg
    16261 gcctcaggtc ccgccgatgg tcatctccgc caccttgatg gtcgggccgt tgacgtagcg
    16321 gcgatggtcc aggtcgtcgc cgatcatctc cacctgctgc agcagcgtcc caagattagc
    16381 accgatggtg attccggcaa ccgggtaagc gagttcgccc ccttcgatcc agatgccgaa
    16441 ggcgccccgg gagatgtcgc ccgtggtggg caccgccccg aacccgatgc tgcccgtgag
    16501 gagcaggccc tcgtccactg aaccgatgat gtccgcagga gaatcctttc ctgacgctag
    16561 atagagattg ttcggccccg ccccgttccc cgtgggcttc atccccagct ttcgagcgga
    16621 gtaggagtcg agcaggaagc tcttgagcac ccctttttca atcaccgccg tcttgcgcag
    16681 ggggaccccc tcgcggtcga agggcttcgc gcccggcgcg cccggcatca gaccgtcgtc
    16741 gaatacgttc acgttcgccc cagccacccg ctcgccgatc cggtcggcca gaaagctctg
    16801 cttgcgatac acaccgcggc catcgacgca gctcgcgaga aactgcaaca gctctgcgct
    16861 catctccggc tccaggacca cgggcacgtt ctgagtagga accttgcggg cgccaatcag
    16921 gcgggtagat cgatcgatgg cctccgccgc gatgtcctcc gggctcctca gcccttcgag
    16981 gttacgggag tagtcgtact ttccctcgct gatgaggttg tcgggctccc ccacctggag
    17041 gtacacgcca ttggaacagg ccgtctgccg gaacgcgtcg ctgaacccgt tggaattcgc
    17101 gagatacagc gtctctatgg tggtgccgaa gccggcaccc atggatcgcc tgattcgttt
    17161 gtcgccaagg caaatggcct ccacgcggcg tgcagccgcg atcttgctct ccggagacat
    17221 gtcgaccacc gcctcgtcga atatgtcgag ggcttcggct cgtaccttga ccggctcctt
    17281 ctccggcaac cctgcaaacg ggtccgcgcc ggacaaccgc gcccgtccga tcccgttgtc
    17341 gatcagcagc ttcaacgtcg cctccgacag atcggatgac gcctccgtgg cgaccttctg
    17401 gtcgacgatc agcttcacct cgagctcccg gaaacctgcc tccaccaggg tttccagctc
    17461 gccttccctg atggacgcag agaagttgct gcccgtgcgg atggcaacct c
//
LOCUS       DBNHQR010000516        10051 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1000883, whole genome shotgun sequence.
ACCESSION   DBNHQR010000516 DBNHQR010000000
VERSION     DBNHQR010000516.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10051)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10051)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10051
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1000883"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="AC16VR_11745"
     CDS             <1..243
                     /locus_tag="AC16VR_11745"
                     /inference="COORDINATES: protein motif:HMM:NF019887.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HIR6011873.1"
                     /translation="AVLAALAETVGTTLERAAYVGDDLPDLGVFSQAGMAISVPNAHP
                     AAAAAADYVTLAPGGAGAAREIAQLFLEARGEWPYG"
     gene            255..884
                     /gene="lptC"
                     /locus_tag="AC16VR_11750"
     CDS             255..884
                     /gene="lptC"
                     /locus_tag="AC16VR_11750"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HIR6011874.1"
                     /translation="MRKRSKWERWSTWTAILGVAVAGVVLLSEPLEPETEAETPPAGI
                     AEEPDLYVEDAVITQYLPSGTVHYRLVSKLLRQFEADELVRLTKPNLVVNSETRSPWQ
                     VQSEHGYVRRMGGPERGGEGEEVVFLREDVQLEQREPDGRHIRLTTETLYVYPEREYA
                     ETDRDVMIDTHAGRTTATGLKGDLQRGLLQLSSSDRRRVHTILLPDQFK"
     gene            898..1380
                     /gene="lptA"
                     /locus_tag="AC16VR_11755"
     CDS             898..1380
                     /gene="lptA"
                     /locus_tag="AC16VR_11755"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HIR6011875.1"
                     /translation="MTLGAVLLAPGALALPEDADQPIHIRANSAEMDQEQGLVIYRGD
                     VQVDQGTLRVMADEMTVEYEDQEVIRITAKGEPARYQQQIEVDEEVVRARSSTIIYHT
                     RDERIDLVGNAFLTQEGNEITGDLIEYDIVAGKVDAKAESETPVRMILQPARKKPDGG
                     "
     gene            1389..2114
                     /gene="lptB"
                     /locus_tag="AC16VR_11760"
     CDS             1389..2114
                     /gene="lptB"
                     /locus_tag="AC16VR_11760"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003313594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HIR6011876.1"
                     /translation="MPTLEVRSLRKQYRNKVAVEDVSLHVSTGEIIGLLGPNGAGKTT
                     TFYMIVGLLRADGGDIFLDEQRITRTPMHVRARAGIGYLPQEASVFRKLSTEDNVRAI
                     LELRRDLNRTQRQAMLDQLIEEFHLGGVRESLGERLSGGERRRLEIARALAMEPSFML
                     LDEPFAGVDPISVGDIKKEIRDLAARGIGVLITDHNVRDTLDICDRAYIVHEGHIIAE
                     GDSGAILADEKVKNVYLGESFSL"
     gene            2275..3750
                     /locus_tag="AC16VR_11765"
     CDS             2275..3750
                     /locus_tag="AC16VR_11765"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014851653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HIR6011877.1"
                     /translation="MKQTLSLKLGQQLTMTPQLQQAIRLLQLSCLDLRQEIQDALEGN
                     PMLELDEEDGGDEPTGSPAEADRAESDGDDDLDGVDATLEGDVIDDLPVDTSWDEIYQ
                     PAPAPAPAAGDSESWDFETRNAPEETLQDHLLWQLNLTPMSDSDRVIAMAIIDTISPD
                     GMLTTPVEELVTSLEGVVDEEAEIAHAEVMAVLKQIQHFDPIGVGARDLGECLRIQLE
                     QLPAGTPWLAESLTLVNEHLPLLASRDFAALKRRTRLGEEELGEVVRLIQTLNPRPGT
                     TVAQEAAEYVVPDVMVSRKEGRWLVELNPDATPGLRINAGYAGLVKRADNSADNTFLR
                     DHLQEARWFLKSLQSRNETLLKVATRIVEHQRGFLEYGEEAMKPLVLNEIAEAVEMHE
                     STISRVTSRKYMHTPRGIFELKYFFSSHVSTSSGGEVSSTAIRALIKKLTADENPRKP
                     LSDSRIATILAEQNINVARRTVAKYRESMAIPPSNERKRLV"
     gene            3807..4112
                     /gene="hpf"
                     /locus_tag="AC16VR_11770"
     CDS             3807..4112
                     /gene="hpf"
                     /locus_tag="AC16VR_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation-promoting factor, HPF/YfiA
                     family"
                     /protein_id="HIR6011878.1"
                     /translation="MQLSISGHHIDVTEALRNYVTEKMQKLERHFDHITNVHVVLSVE
                     KLRHRAEATIHASGTEVFADCDCDDLYAAIDGLTDKLDRQVLKHKEKLLDRQQRRGR"
     gene            4122..4589
                     /locus_tag="AC16VR_11775"
     CDS             4122..4589
                     /locus_tag="AC16VR_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011532414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR6011879.1"
                     /translation="MPDITTILRPDCTRASVEAPSRKRALEAAADLVAKHHPDLSARK
                     LFDELMARERLGSTGLGDGVAIPHCRIPCEQITGALLHLREAVDYESMDDEPVDLLFV
                     LAVPPEETTAHLDLLAALARIFGDPENRRRLRSSATDEALYQELVGLFSSQAA"
     gene            4586..5440
                     /gene="rapZ"
                     /locus_tag="AC16VR_11780"
     CDS             4586..5440
                     /gene="rapZ"
                     /locus_tag="AC16VR_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HIR6011880.1"
                     /translation="MKLIVISGRSGSGKSTALRALEDSGYNCIDNFPVDLLPHLIANA
                     TRAVEPQPDLAVCIDARNPDLEAFPQIIESLRSPSVDCEVIFLDAQSATLVKRFSETR
                     RRHPLTGTGKDLLQAIESEDAILQPIAGLADLSIDTTTMRGSALTELVRSRIAHNRSN
                     SLSLLFRSFGFKFGVPVDADFVFDIRCLPNPYWDESLRPLTGKDGAVASFLEAEAAVG
                     QMFSDIQSFLERWIAAFEKDNRAYMNIALGCTGGQHRSVYMAEKLAAHFSAQRPNVLV
                     RHRELAGS"
     gene            5448..5717
                     /locus_tag="AC16VR_11785"
     CDS             5448..5717
                     /locus_tag="AC16VR_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-carbohydrate
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR6011881.1"
                     /translation="MREGRITIVNRLGLHARAAAKFVNLAKTFSSQVELTRDAERVDG
                     KSIMSVMLLAAPIGTELILHVVGPDEDEAFEALEQLVDDRFGEAE"
     gene            5769..7106
                     /gene="mgtE"
                     /locus_tag="AC16VR_11790"
     CDS             5769..7106
                     /gene="mgtE"
                     /locus_tag="AC16VR_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIR6011882.1"
                     /translation="MDFQEQLSEVIRQYGSGSLDETRFLLESLRAPEIANLMESTPPR
                     VRRVIWGLLDEEDENQVIQHLREDVRADFLESMNTERLVAAGVDLEVDDFADILQQLP
                     ATLSEQVLASLDQSDRARVETVLSYPEDSAGGIMNTDAITVRPRHSVELVLRYLRLRK
                     DLPEATDALIVVNQRDEYVGMVSLASLITSDPTVTVREVMNTEARSIPADTPDTEVAR
                     LFSEQDLISAPVVDQQGRLIGRITIDDVVDVIIEDADEAVLARAGLDLEDDTFAPIMK
                     SVRGRALWLGVNLLTTFVAAAVINVFEETIAKVVALAVLMPIVASMGGIAGTQTLTLV
                     IRGIAVGQLGRANLPYLLNREFLVAALNGLMLAIVVAVAAAVAFGDTVLGLVIAIAML
                     INLLVAAVSGTLLPSILNALRIDPAIAGGVVLTTITDVTGFFTFLGLATWAYA"
     gene            complement(7124..8470)
                     /gene="pmbA"
                     /locus_tag="AC16VR_11795"
     CDS             complement(7124..8470)
                     /gene="pmbA"
                     /locus_tag="AC16VR_11795"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HIR6011883.1"
                     /translation="MPNELIDLQDSEAGLSSLVGDILDEARRQGADAAEVSVNEDAGL
                     SVSVRKGELETVEFNQDRGFGITVYYGLRKGSASTSDSSLTAIEDTVRAARDIARHTE
                     EDPCNGLADAGLMPDALPELDLYHPWRLDVGEAERLAIACEAAGLSRDERIVNSDGAQ
                     VSTQQSCRVYGNSHGFIGSYLGTRHSVSCVLIAADESGMQRDYWYSLARDPEGLEDME
                     SVGVEAARRVVARLSPRKPLTGRFPVVFSAQMSAGLVGHLVGALSGGSLYRNASFLLD
                     SLGHGVAAPHLSVLEEPHLPGRIGSSGFDGDGVGTSAKHFVEDGVVRSYVLSTYSGRK
                     LGMPTTGNAGGVFNLLLRGRTLAVDDLYRSMGSGLLVTELMGQGVNLVTGDYSRGAAG
                     FWVENGAIAYPVHEVTIAANLKDMLLDVQALGDDVDERGNVRTPSVLVGSMMVAGS"
     gene            8512..8997
                     /gene="yjgA"
                     /locus_tag="AC16VR_11800"
     CDS             8512..8997
                     /gene="yjgA"
                     /locus_tag="AC16VR_11800"
                     /inference="COORDINATES: protein motif:HMM:NF016626.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HIR6011884.1"
                     /translation="MTEPPISKSERKRAAARLQEIGRALAELRPARLDEIPLPETLER
                     AIYDYRRFASREAKRRQLQRIGKLMREIDTAPIEAALERLEGRSASARYEQHQLERWR
                     DRLIEDPAALTDYLAEHPHSERQTLRHLIERARTAGNEQQQKTASRALFRFLREDVPT
                     G"
     gene            complement(9044..>10051)
                     /gene="tldD"
                     /locus_tag="AC16VR_11805"
     CDS             complement(9044..>10051)
                     /gene="tldD"
                     /locus_tag="AC16VR_11805"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015475821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HIR6011885.1"
                     /translation="AARQADPRVKEVNVRLSASHESILVMASDGTLCADVRPLVRLDV
                     SVIVEQDGRRERGSAGAGGRGGLGLVSDSEFTDGLAREAVRTALVNLEAVDAPAGPMP
                     VVLGHGWPGVLLHEAVGHGLEGDFNRKGSSAFANRVGEKVASELCTVVDDGTLDGRRG
                     SLTVDDEGSPSQETVLIENGVLRGYMQDKLNARLMKTSSTGNGRRQSFAHLPMPRMTN
                     TFMKAGEHDPEEIIASVDRGIYAVNFGGGQVDITSGRFVFSATEAYLIEKGRVTHPVR
                     GATLIGNGPEVMTRISMVGNDLELDRGIGVCGKDGQSVPVGVGQPTLKIDEVTVGGTS
                     A"
BASE COUNT         1881 a         3428 c         3155 g         1587 t
ORIGIN      
        1 gccgtcctgg cggccctggc cgagacggtc ggcaccaccc tcgagcgagc tgcctatgtc
       61 ggcgacgatc tgccggacct cggcgtgttc tcccaggccg gaatggccat cagcgtaccc
      121 aacgcgcacc cggccgcggc ggccgccgcc gactacgtca ccctggcccc cgggggtgca
      181 ggggcggcgc gggagatcgc ccagcttttc ctggaagccc ggggcgagtg gccctacggg
      241 taaccggctg agccatgcgc aagcgctcca aatgggaacg atggtcgacg tggacggcca
      301 tcctgggtgt tgcggtggcc ggggtcgttc tgctgagcga gcccctggag ccggagacag
      361 aagcggagac cccgcccgcc ggcatcgccg aagagccgga tctttacgtc gaagacgccg
      421 tcatcaccca gtacctgcca agcggaaccg tccactaccg gctggtgtcg aaattgctga
      481 ggcagttcga ggcggacgag ctggtgcggc tgaccaagcc gaacctggtg gtcaacagcg
      541 aaacgcgctc gccgtggcaa gtgcagtcgg agcacggcta cgtgcgccga atgggcggac
      601 ccgagcgtgg cggcgaaggc gaagaggtgg tttttctgcg cgaggacgtc cagctagagc
      661 agcgggaacc cgacggtcgc cacatacgcc tgaccacgga gaccctctat gtgtatccgg
      721 agcgagagta cgccgagact gaccgcgatg ttatgatcga cacccatgcc ggccggacca
      781 cggccaccgg actcaaaggt gacttgcagc gtggattgct acagctgtcc tcctcagatc
      841 gacgccgcgt gcacacgatt ctgctgccgg atcagttcaa gtaggctgct gcgtgccgtg
      901 acgcttggcg ccgtcctgct cgctccgggc gcgctcgctc tgcccgagga cgccgaccag
      961 cccattcaca tacgcgccaa cagcgcggag atggatcagg aacaggggct ggtgatctat
     1021 cgcggcgacg ttcaggtgga ccaggggact ctgcgcgtca tggccgacga gatgaccgtg
     1081 gagtacgagg atcaggaggt gatacgcatc accgccaagg gagagcccgc ccgctaccag
     1141 cagcagatcg aggttgacga ggaagtggtt cgcgctcggt cgtcgaccat catctaccac
     1201 acgcgcgacg agcgcatcga cctggtcggc aacgcgtttc tcacccagga aggcaacgag
     1261 atcaccgggg acctcatcga gtacgacatc gtcgccggca aggtggatgc gaaggcggaa
     1321 agcgaaacgc cggtgcggat gatccttcag cccgctcgca agaaacccga cggcgggtaa
     1381 acggccccat gcccacactg gaagttcgct ctctacgcaa acagtaccgt aacaaggtcg
     1441 cggtggaaga cgtcagcctg cacgtcagca ccggcgagat catcggcctg ctgggaccca
     1501 acggtgccgg caaaaccacc accttctaca tgatcgtcgg cctgctgcga gccgacggcg
     1561 gcgatatctt tctcgacgag cagcggatca cccggacccc catgcacgtg cgcgcccggg
     1621 cgggtatcgg ctatctgccc caggaagcaa gcgtcttcag aaagctcagc acggaggaca
     1681 acgtgcgggc catcctggag ctgagacggg acctgaaccg gacccaacgc caggccatgc
     1741 tcgatcagct gatcgaggag tttcacctgg gcggggtgcg cgagtccctc ggcgagcggc
     1801 tctccggcgg cgagcgacgc cggctggaga ttgcccgggc actcgccatg gagcccagct
     1861 tcatgctgct cgacgagccg ttcgcgggcg tcgaccccat ttccgtcggc gacatcaaaa
     1921 aggagatccg cgatctggcg gcccgcggca tcggcgtgct catcaccgat cacaacgtgc
     1981 gggacaccct ggacatttgt gaccgggcct acatcgttca tgagggccac atcattgcag
     2041 aaggcgattc tggcgccata cttgcagacg agaaggtcaa aaacgtatat ctgggcgaga
     2101 gcttctctct ttgagcctgg ctccttgaga tcagccctca gttccaggcc gcgatagcgc
     2161 ggccgcacag ccaggggcga gcgtcaggca cgccaagcct tttgacccgt taggacaaca
     2221 accggcaacc atgccgcgcg gaaggacgac cgctcaccag agccctcggt ttgcatgaaa
     2281 cagactctgt cgctcaagct cggacagcag ctcaccatga cgccgcagct gcagcaggcc
     2341 atccggctgc tgcagctctc ttgcctggac ctgcgtcagg aaattcagga tgccctggaa
     2401 ggcaacccga tgctcgaact cgacgaggag gacgggggcg acgagccgac gggctctccc
     2461 gcagaggccg atcgggccga aagcgacggt gacgacgacc tcgacggcgt ggatgcgacc
     2521 ctggaagggg acgttatcga cgatctgccg gtcgacacga gctgggacga gatctaccag
     2581 cccgcgcctg ctcccgctcc ggcagccggc gattccgaat cctgggattt cgaaacgcgg
     2641 aacgcgcccg aggagaccct gcaggaccat ctgctctggc agctgaacct cacgcccatg
     2701 tccgactccg atcgggtgat cgccatggcg atcatcgaca ccatcagtcc ggacgggatg
     2761 ctgaccacgc ccgtggaaga gctggtgacg tctctggaag gcgtggtgga cgaggaggcg
     2821 gagattgccc acgccgaggt catggccgtg ctcaagcaga tccagcattt cgaccccatc
     2881 ggggtgggcg cccgggacct gggcgagtgc ctgaggatcc agctcgagca gctgccggcg
     2941 ggcacgccgt ggctcgcgga gtcgctgacc ctcgtcaacg agcacctgcc tctgctggcc
     3001 agccgggact tcgccgccct gaagcgccgg accaggctgg gagaggagga gctcggcgaa
     3061 gtcgtccggc tgattcagac gctcaatccg cgcccgggca ccacggtggc ccaggaggcg
     3121 gcagagtacg tcgtccccga cgtcatggta tccaggaaag aggggcgctg gctggtggag
     3181 ctcaaccccg acgcgacgcc gggcttgaga atcaacgccg gctacgccgg gctcgtcaag
     3241 cgcgccgaca acagcgccga caacaccttc ctgcgcgatc atctgcagga agcgcgatgg
     3301 ttcctcaaga gcctgcagag ccgcaacgag accctgctca aggtcgccac ccgcatagtg
     3361 gaacatcagc ggggattcct ggaatacggg gaagaagcca tgaagcccct ggtgctcaac
     3421 gagatcgccg aggccgtgga aatgcacgaa tcgaccatct cccgggtcac tagccgcaag
     3481 tacatgcaca cgccccgggg catcttcgag ctgaaatatt tcttttccag ccacgtgtcc
     3541 acgagcagcg gcggcgaggt ttccagcacg gccatccgcg cgctcatcaa gaagctgacg
     3601 gccgatgaaa atccaagaaa accgttgagc gacagccgca tcgctacgat actggcggag
     3661 cagaacatca acgtcgccag acgcaccgtg gccaaatatc gagagtcgat ggccatacca
     3721 ccgtccaacg aacgcaagcg tctggtttga tcattcatga cggcacacga cgcgactcga
     3781 catcaacagc aaaacaagga gccagcatgc agttgagcat ctccggtcat cacatcgacg
     3841 tcaccgaggc gctgcggaac tacgtgaccg aaaaaatgca gaaactggaa cgccacttcg
     3901 accacatcac caacgtccac gtggttctga gcgtcgagaa gctgcgccat cgcgccgagg
     3961 cgaccataca cgcgagcggc accgaggtgt tcgccgactg cgattgcgac gacctctacg
     4021 cggcaatcga cgggctcacg gacaaactcg accgtcaggt cctcaagcac aaggagaagc
     4081 tgctggaccg acaacaacgc cggggacgct gagcgggggc catgccggac atcaccacca
     4141 tactcagacc ggattgcacg agagcttccg tcgaggcgcc ttcgcgcaag cgcgctttgg
     4201 aggcggccgc agacctggtg gccaagcatc atccagatct gtccgcgcgc aagctgttcg
     4261 acgagctcat ggcgcgcgag cggctcggca gcacggggct cggcgacggc gtcgccatcc
     4321 cccactgcag gattccgtgc gagcagatca ccggcgccct gctccacctg cgagaggccg
     4381 tggactacga gtccatggac gacgagccgg tggatctgct gtttgtgctc gccgtgccgc
     4441 ccgaggaaac gacggcccat ctggacctgc tggccgccct ggcgcggatt ttcggcgatc
     4501 cggaaaatcg cagacgcctg cgcagcagcg caacggatga agccctctat caggaactgg
     4561 tggggctctt cagcagtcag gcggcatgaa gctcatcgtc atcagcggtc ggtccgggtc
     4621 gggcaagagc acggccctgc gagcgctgga ggattccggc tacaactgca tcgacaactt
     4681 ccccgtcgac ctgctccccc acctgatcgc gaacgccaca cgggccgtcg agccgcagcc
     4741 ggacctggcc gtgtgcatcg atgcccgcaa tccggacctg gaagcctttc ctcagatcat
     4801 cgagtcgctg cgctccccca gcgtggactg cgaggtgatc ttccttgacg cccagagcgc
     4861 gacgctggtc aaacgcttca gcgagacccg ccggcgccac ccgctgaccg gcacgggtaa
     4921 ggacctcctc caggccatcg aatcggagga tgcgatcctg caaccgattg ccggcctggc
     4981 ggatctcagc atcgacacca ccaccatgag aggcagtgcc ctgaccgagc tcgtgcgcag
     5041 ccgcatcgcc cacaaccgca gcaactcgct ctctcttctg ttccgatcat tcgggttcaa
     5101 gttcggtgtg cctgtggacg ccgacttcgt cttcgacatc cgctgcctgc ccaatcccta
     5161 ctgggacgag tccctgcgtc cgctgaccgg caaggacggg gcggtggcgt cgtttctgga
     5221 agcggaagcg gccgttggcc agatgttctc ggacatccaa tcgttcctgg aacgctggat
     5281 tgccgccttc gagaaagaca accgggcgta catgaacatc gccctgggct gcaccggcgg
     5341 ccagcaccgc agcgtgtaca tggcggaaaa gcttgccgcc cacttctcgg cccagcggcc
     5401 caacgtgctg gtcaggcacc gcgagctggc aggttcgtaa ccccgccatg cgggagggaa
     5461 ggatcaccat agtcaaccgt ctcgggctgc acgcccgggc cgcggccaag ttcgtcaatc
     5521 tggcaaagac cttctcgagc caggtggagt tgacgaggga cgccgagcgc gtggacggca
     5581 aaagcatcat gagcgtcatg ctgctcgccg cgcccatcgg caccgagctg atcctgcacg
     5641 tcgtcgggcc ggacgaggac gaggccttcg aagccctgga gcaactcgtc gacgaccgct
     5701 tcggagaagc tgagtgaacg gccggcgcat ctggtaatct gcgcgcgccc tcaagtttcg
     5761 gtacccgcgt ggactttcag gaacagctaa gcgaagtcat ccgccaatac ggcagcggaa
     5821 gtctggacga gaccagattt ctgctcgaat ccctgcgcgc gccggaaatc gccaacctca
     5881 tggagtccac cccgcccaga gtccgccggg tcatctgggg gctcctggac gaagaggacg
     5941 agaaccaggt cattcagcat ctccgcgagg acgttcgggc cgattttctg gagtccatga
     6001 acaccgagcg gcttgtggcc gccggcgtcg acctggaggt cgacgacttc gcggacatcc
     6061 tccagcagct gccggcaacc ctttccgagc aggtcctggc atccctcgat cagagcgacc
     6121 gggcccgggt ggagaccgtg ctctcctatc cggaggacag tgccggcggc atcatgaaca
     6181 ccgacgcgat caccgtgcgg ccacggcact ccgtggagct ggtgctgcgc tacctgcgcc
     6241 tgcgaaagga cctgccggag gccaccgacg cgctcatcgt cgtgaaccag cgggacgagt
     6301 acgtcggtat ggtgtcgctg gccagcctga tcacctcgga ccccaccgta accgttcgcg
     6361 aggtgatgaa taccgaagcg cggtcgatcc ccgccgacac gccggacacc gaggttgcgc
     6421 gcctgttctc cgagcaggat ctgatctccg cgcccgtcgt ggatcagcaa gggcgcctga
     6481 tcggccggat caccatcgac gacgtcgtcg acgtcatcat cgaggacgcc gatgaagcgg
     6541 tgctcgcccg ggccggtctg gacctggagg acgacacctt cgcgcccatc atgaagtccg
     6601 tgcgcggccg cgcactgtgg ctcggcgtca acctgctgac caccttcgtg gccgcggcgg
     6661 tcatcaacgt gttcgaggaa accatcgcca aggtggtggc gctggccgtg ctgatgccca
     6721 tcgtcgccag catgggtggc atcgcgggca cgcagacgct gacgctggtc attcgcggca
     6781 ttgccgtggg gcagctgggt cgcgccaacc tgccctatct gctcaatcgg gaatttctgg
     6841 tcgccgcgct gaacggcttg atgctcgcca tcgtggtagc cgttgccgcc gctgtcgcct
     6901 tcggcgacac cgtgctgggc ctggtcatcg ccatcgccat gctgatcaac ctgctggtcg
     6961 ccgccgtgtc gggaaccctg ttgcccagca ttctcaacgc cctgcggatc gatccggcca
     7021 tcgccggcgg cgtggtgctg accacgatca ccgacgtgac cggatttttc acctttctcg
     7081 gtctcgcgac ctgggcctac gcctgacccg gcgacacccc ggtttacgac cccgcgacca
     7141 tcatggagcc gacgagcacg ctgggcgtgc gcacgttccc gcgttcgtcc acgtcgtcgc
     7201 ccagcgcctg gacgtccagc agcatgtcct tgaggttggc ggcaatggtg acctcgtgca
     7261 ccggataggc gatcgccccg ttctctaccc agaaccccgc ggcaccccgc gagtagtcac
     7321 cagttaccag gttgacgccc tgacccatca gctccgtgac caggagcccg gagcccatgg
     7381 agcggtaaag gtcgtccacg gccagcgttc ggcccctcag cagcaggttg aaaacacccc
     7441 cggcgttgcc cgtcgtgggc atgccgagct tccgacccga gtaggtggac aggacgtagc
     7501 tgcgcacgac gccgtcctcc acgaaatgct tcgccgaggt gcccaccccg tcaccgtcga
     7561 aacccgagct gccgatgcgg ccgggcaggt ggggttcctc gaggacgctc aggtgaggcg
     7621 cggcgacgcc gtggcccagc gaatcgagga gaaacgacgc gttccggtag agactcccac
     7681 cgctcaacgc cccgaccagg tgccccacca gaccggccga catctgcgcc gagaagacga
     7741 cgggaaagcg gccggtcagc ggcttgcggg gcgacagccg tgccaccacc ctgcgggcgg
     7801 cttcgacgcc cacgctctcc atgtcctcca gcccctccgg atcccgagcc aggctgtacc
     7861 agtaatcacg ctgcatgccc gattcatcgg ccgcaatcag cacacagctg acgctgtgtc
     7921 tggttccgag gtagctgccg atgaacccgt gactgttgcc gtagacccga cacgactgct
     7981 gcgtgctcac ctgcgccccg tcggagttca cgatgcgctc gtcgcggctg agcccggccg
     8041 cctcgcaggc gatcgccagc cgctcggcct cgccgacatc caagcgccag gggtgataca
     8101 gatccagctc cggcagcgca tccggcatca aaccggcgtc cgccaggccg ttgcaggggt
     8161 cttcctcggt gtggcgggca atgtcccggg ccgcgcgcac ggtgtcctcg atggccgtca
     8221 ggctgctgtc cgacgtgctc gccgagccct tgcgcaaacc gtagtagacg gtgattccaa
     8281 atccccggtc ctggttgaac tcgacggttt ccagctcacc tttgcgcacc gaaacggaaa
     8341 ggccggcgtc ctcgttcacg gacacctcgg cggcgtccgc gccctggcga cgggcttcgt
     8401 ccaggatgtc gccgaccagg gacgaaagcc ccgcctcgct gtcttgcagg tcaatcaact
     8461 cgttcggcat ggagtcctca catttgcctc tgcttcgggt acgctcgacc gatgaccgag
     8521 cctcccatca gcaaatccga acgcaaacgc gccgcagcgc gcctgcagga gatcggccgg
     8581 gccctggccg agctgcgtcc cgccaggctg gacgagatcc ccttgccgga aacgctcgag
     8641 cgcgccatct acgactaccg gcgatttgcc tcccgggagg ccaaaagacg ccagctccag
     8701 cgcatcggca agctcatgcg tgagatcgac accgccccga tcgaggcggc cctggagcgt
     8761 ctcgaggggc gctcggccag cgccaggtac gagcagcacc agctggaacg gtggcgcgac
     8821 cggctgatcg aggatcccgc cgcgctcacc gactacctcg ccgaacatcc gcactccgag
     8881 cgccagacgc tgcggcacct gatcgagcgg gcccgcacgg caggcaacga gcagcagcag
     8941 aagacggcat cccgagccct gttccgcttt ctgcgggaag acgtgcccac cggataggca
     9001 cgctcggcgg gcgacgcgcg cgcggcccca atcgccattg cgttcacgcg ctcgttccgc
     9061 cgacggtgac ctcgtcgatc ttcaacgtgg gctggcccac gcctaccgga accgactgcc
     9121 cgtccttgcc gcaaacgccg atgccgcgat cgagctcgag gtcgttacca accatggata
     9181 tcctggtcat gacctcgggc ccgttgccga tgagggtggc accacggaca ggatgggtca
     9241 cgcgaccctt ctcgatgaga taggcctcgg tggctgagaa aacgaacctg cccgaggtga
     9301 tgtccacctg accgccgcca aagttgacgg catagatgcc gcggtcgacg ctcgcgatga
     9361 tctcctcggg atcgtgctcc ccggccttca tgaaggtatt ggtcatccgc ggcatgggca
     9421 ggtgcgcaaa agactggcgg cgcccgttac ccgtgctcga tgtcttcatc agcctggcat
     9481 tgagcttgtc ctgcatgtag ccccgcagca cgccgttctc gatgagcacc gtttcctggc
     9541 tgggcgagcc ctcgtcgtct acggtcagcg acccacggcg cccgtcgagg gttccgtcgt
     9601 ccaccaccgt gcacagctcg ctcgcgactt tctcgccaac gcggttggca aacgccgagc
     9661 tgcccttgcg gttgaagtcc ccttccagac cgtgccccac cgcctcgtgc agcagcaccc
     9721 cgggccagcc gtggccgagc acgaccggca tgggtcctgc cggcgcgtcg acggcttcca
     9781 gattcaccag cgccgtacgc accgcctccc gggccaggcc gtccgtgaat tcagaatcgc
     9841 tgaccaggcc gaggcccccg cggcctccgg ctccggcaga gccccgctcc cgacggccgt
     9901 cctgctcgac gatcaccgag acgtccaagc gcaccagcgg cctgacatcg gcacagagcg
     9961 tgccgtcgga ggccatcacc aggatcgact catggctcgc cgagagacga acattcacct
    10021 ctttgacgcg cgggtctgcc tgccgggccg c
//
LOCUS       DBNHQR010000517         4307 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_113495, whole genome shotgun sequence.
ACCESSION   DBNHQR010000517 DBNHQR010000000
VERSION     DBNHQR010000517.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4307)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_113495"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1216
                     /gene="uvrC"
                     /locus_tag="AC16VR_11810"
     CDS             <1..1216
                     /gene="uvrC"
                     /locus_tag="AC16VR_11810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HIR6011886.1"
                     /translation="SNAVLEAFKGNMQKAAEAMDYEAAAKYRDQIAHLRKVQEQQYVH
                     AAEGDVDVFALMEASGVCCVQALFVRAGRMLGQRTWFPKNELGLEPAEMLSAFLSQYF
                     LAGAERDVPRTVITAERIVDQDLLADALSKRSGRRVDVLSQVRGQRARWVRLGLDNAA
                     HSLNAYLADKRNVYARLVALQDLLGLEDVPERLECFDISHTMGEATVASCVVFDRNGP
                     LKSDYRRFNIEDVAAGDDYAAMEQALRRRYTRLKQGEGKLPDVLVIDGGRGQLQRAKD
                     VLDELQVDGVHLLAIAKGPSRKPGLEKLFANGAEVDYPEQGAAAHLLQHIRDEAHRFA
                     ITGHRQRRQRKRSRSELDDVAGVGPKRRRELLTHFGGLSGVKGASVEEIAKVPGISRR
                     LAEEIYGTLHAE"
     gene            1253..1852
                     /gene="pgsA"
                     /locus_tag="AC16VR_11815"
     CDS             1253..1852
                     /gene="pgsA"
                     /locus_tag="AC16VR_11815"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012315213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HIR6011887.1"
                     /translation="MNLPNLLTLGRILFVPVFVLVYVIPGERTYLLAATLFALAAFTD
                     WLDGYLARRLNQTTPFGAFLDPVADKLIVVTALVLLIGHHRTLWLTLPALVIVGREIV
                     ISALREWMAEMNRRGVVAVSWLGRVKTTFQMIAVVVLLANPPAFDRPWVLLGYGLLYV
                     AAAMTLASMALYLHAAWPTLKSGYGGPEGEPGEDPRDRS"
     gene            1923..1996
                     /locus_tag="AC16VR_11820"
     tRNA            1923..1996
                     /locus_tag="AC16VR_11820"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1955..1957,aa:Cys,seq:gca)
     gene            2170..3378
                     /locus_tag="AC16VR_11825"
     CDS             2170..3378
                     /locus_tag="AC16VR_11825"
                     /inference="COORDINATES: protein motif:HMM:NF019640.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6011888.1"
                     /translation="MSGTLLERVASLKSVIEEGGDEAQKLRRVPDETIRTLVDSGFFR
                     FTLPEELGGENATIRETIEVLEAISAIDGSVGWNVMLGSEINAMAAGGMDKELAKEVY
                     LDNPRVIMCGGGGPGSMPTKAVAQEDGSYRVWGQTTFISGCHNAEWCFMAAPIVENDA
                     PKMHENGMPMVRMWFLHRSEWEIVDTWDVAGLRGSGSHDVRAEGALVPERFVPVQLVS
                     LPAHYPNPVYRVPVPLRLAYNKAAVAIGVARGALDTFADLAQSKIPMLSSSTLRDRPI
                     AQYKMGECEAKLRAARAFLMEAMDAVHDELEAGAPTPSAERTYMGRLACTYAANASME
                     VVDVIHNAAGTSGMRMYSPLERKLRDAHGCATHRWVAHPLYQDLGKIFLGHEPAPEFA
                     GEGDPMQGKK"
     gene            3441..3584
                     /locus_tag="AC16VR_11830"
     CDS             3441..3584
                     /locus_tag="AC16VR_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011889.1"
                     /translation="MEAAFLMSQLPLLRLEAPRWRGPVLLLPGIMADVYRLISRILAR
                     PGS"
     gene            complement(3595..4041)
                     /gene="phaR"
                     /locus_tag="AC16VR_11835"
     CDS             complement(3595..4041)
                     /gene="phaR"
                     /locus_tag="AC16VR_11835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HIR6011890.1"
                     /translation="MRELKKYPNRRLYDLDESHYVTVDDVRQMIVAGESIRVLDSKDG
                     SDITRSILLQILAEQEAEGHEPILTNRAIEQIIRFYGQNFGSVVSRYIEQSILTFLEH
                     QDQFRRRMREMSDLNPLNFVRQAMERTFGDTDRDGGRDGGRNKGDK"
     gene            complement(4044..>4307)
                     /locus_tag="AC16VR_11840"
     CDS             complement(4044..>4307)
                     /locus_tag="AC16VR_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HIR6011891.1"
                     /translation="PEASLSVPTTAIYSKSDGVVHWSSCLELDGEQSENVEVLGSHSG
                     MAHNPLVFHVLADRLAQPEGEWRPFQRGKGCRALLFPKPEARS"
BASE COUNT          758 a         1326 c         1443 g          780 t
ORIGIN      
        1 aagcaacgcg gtgctagagg ccttcaaggg gaacatgcag aaagcggcgg aagcaatgga
       61 ctacgaagcc gcggcaaagt accgggatca gatcgcccac ctgcgcaagg ttcaggagca
      121 gcagtacgtt cacgccgcgg aaggagacgt ggacgtcttc gccctgatgg aggcttccgg
      181 cgtgtgctgc gtgcaggcgc tgttcgtgcg cgcggggcgc atgctggggc agaggacgtg
      241 gtttccaaag aacgagctcg gtctggagcc ggccgagatg ctgagcgctt ttctgtctca
      301 gtactttctg gccggcgctg aacgggacgt gccgcgcacg gtgatcaccg cggagcgcat
      361 cgtcgaccag gatcttctcg ccgacgcgct ttccaagcgg tccgggcgcc gggtggacgt
      421 cctctcccag gttcgcggcc agcgcgcccg ctgggtgagg ctgggtctcg acaacgcggc
      481 ccacagcctg aacgcctatc tggccgacaa gcgcaacgtt tatgcgcggc tggttgctct
      541 tcaggatctg ctgggcctgg aggacgtgcc ggaacgcctg gagtgcttcg acatcagcca
      601 caccatgggc gaggccacgg tggcgtcctg cgtcgtcttc gaccgcaacg ggccgctcaa
      661 gtcggactat cggcgattca acatcgagga tgtggcggcc ggcgatgact acgcagctat
      721 ggagcaagcg ctccggcgtc gatacactcg cttgaagcag ggcgaaggaa agctgcccga
      781 cgtgctggtg atcgacggtg gccgcgggca actgcagcgg gctaaggacg ttctcgacga
      841 gttgcaggtc gacggcgtgc acctgctggc catcgccaaa gggcccagca gaaagccggg
      901 actggaaaag ctgtttgcta acggcgcgga ggtggactac ccagagcagg gggctgccgc
      961 tcatctgctt cagcacattc gcgacgaagc gcaccgcttt gccatcaccg gccaccgcca
     1021 acgccgtcag cgcaaacgca gccgatcgga gctcgatgac gtcgccggcg tggggccgaa
     1081 gcggcggcgc gagctgttga cccacttcgg tgggctctcc ggcgtgaaag gtgccagcgt
     1141 cgaggagatc gccaaggtgc ccggaatcag ccgcaggctg gcggaagaga tctacggtac
     1201 cctgcacgcg gagtgatcgt tacgcggtga gcacacagcc atgaaaccgg tcatcaactt
     1261 acccaatctg ctcacgctgg ggcgaattct tttcgtgccg gttttcgtgc tcgtctacgt
     1321 catcccggga gaacgtactt acctcctggc agccacgctg tttgcgctgg ccgcattcac
     1381 cgactggctg gacggttacc tggcccgacg cctcaaccag accacgccct tcggggcgtt
     1441 tctcgacccg gtggcggaca agctcatcgt ggtcaccgct ctcgtgcttc tcatcggcca
     1501 ccaccgcacg ctgtggttga ccctcccggc gctggtgatc gtcgggcgag agatcgtcat
     1561 cagcgcgctg cgcgagtgga tggcggaaat gaaccgccgc ggcgtcgttg cggtttcctg
     1621 gctggggagg gtgaaaacca cctttcagat gatcgcggtg gtcgtgctgc tggccaaccc
     1681 gccggctttc gatcggccct gggtgctgct cggctatggg ctcctttacg ttgccgccgc
     1741 catgaccctc gcctccatgg ccctgtatct gcatgctgcc tggccgacgc tgaagtccgg
     1801 ttacggcggc ccggaaggcg agcccggcga agaccctcgc gatcgctcct aaagcccgct
     1861 agaattcgcc ggcgtgttcg cagggcgacc gcgactgacg tcgcgcgccg gccggcgacg
     1921 aaggctgagt ggcagagtgg tcatgcagcg gactgcaact ccgtgtacgc cggttcgatt
     1981 ccgacctcag cctccaaatc tcccacggga ctcgaaaccc gtccacccgc cgctgcggcg
     2041 ggggccgggc gtgcgcccgc gggaacgtga gacatccgcg aagggttgtg taaaatcctg
     2101 gtatgcggag agttgagagg tagacgagaa gtggctgagc agatcgcaac agaagaactg
     2161 ataccacccg tgagcggaac gttgctggaa cgggtcgcgt cgttgaagag cgtcatcgaa
     2221 gagggtggcg acgaagccca gaaactgcgg cgcgtgccgg atgagacgat ccggacgctg
     2281 gtggattccg gatttttccg tttcacgcta cccgaagagc tcggcggtga gaacgcgacc
     2341 attcgcgaga ccatcgaggt gctggaggca atctcggcca ttgacggctc cgttggctgg
     2401 aacgtcatgc tgggctcgga gatcaacgcc atggccgccg ggggtatgga caaggagctg
     2461 gccaaggaag tctatctgga caaccctcgg gtgatcatgt gcggcggcgg cgggccgggc
     2521 agcatgccca ccaaggcggt ggcccaggag gacggcagtt accgggtgtg gggacagacc
     2581 accttcatca gcggctgtca caacgccgag tggtgcttca tggccgcgcc gatcgtcgag
     2641 aacgacgcgc cgaagatgca cgagaacggc atgcccatgg tgcgcatgtg gttcctccat
     2701 cgaagcgaat gggaaatcgt ggatacctgg gacgtcgccg ggctgcgagg gtccggaagc
     2761 cacgacgtcc gggccgaggg cgcgctcgtt ccggagcggt tcgttcccgt tcagctggtg
     2821 tcgcttcccg ctcactatcc caatcccgtc tatcgggtgc cggtgccgct tcgccttgcc
     2881 tacaacaagg cagcggtcgc catcggcgtt gcccggggcg ctctggatac cttcgccgat
     2941 ctggcgcagt cgaagattcc gatgctctcg tcttccaccc tgcgggaccg gcccattgcc
     3001 cagtacaaga tgggggagtg tgaggccaag cttcgcgcgg cccgggcttt cctcatggag
     3061 gccatggacg cggtgcatga cgagctcgag gcgggggcac ccaccccatc cgcggaacgc
     3121 acctacatgg gacggcttgc ctgcacgtac gctgcgaacg cgtccatgga agtcgtggac
     3181 gtcattcaca acgcggccgg taccagcgga atgcgcatgt acagccctct ggagcgcaag
     3241 ctgagggacg ctcacggctg tgcgacccat cgctgggtag cccatccgct ttaccaggac
     3301 ctgggcaaga tttttctcgg tcacgagccg gcgccggagt tcgcgggcga gggcgatccc
     3361 atgcagggca agaaatagcc gaccgcgagt tcattgctcc gccgggacga ctgaaccagc
     3421 tgctggaggg gcgtgcggcc gtggaagccg cgtttctcat gagccagctg ccgttgctgc
     3481 gcctcgaggc gcctcgctgg cgtggccccg tgctgctcct tcctggcatc atggccgacg
     3541 tctatcgcct gatttccaga atcctcgcga ggcccggcag ctgaagggca ggggctactt
     3601 gtctcccttg ttgcgccccc cgtcgcgccc cccatcgcga tcggtatcgc caaacgttcg
     3661 ttccatggcc tggcggacga aattgagcgg gttgaggtcg gacatttcgc gcatcctgcg
     3721 gcgaaactga tcctggtgct ccaggaaggt caggatgctc tgctcgatat atctggacac
     3781 gacgctgccg aaattctgtc cgtagaaacg gatgatctgc tcgatggccc ggttggtcag
     3841 aatgggctcg tggccttcag cctcctgttc cgcaaggatc tgcagaagga tcgatcgggt
     3901 gatgtcggag ccgtccttgc tgtcgagcac gcggatgctt tcgcctgcga cgatcatctg
     3961 acgaacatcg tccacggtca cgtagtgact ttcgtcgaga tcgtaaaggc gccggttggg
     4021 atacttcttg agctcgcgca tcctcaggaa cgcgcttccg gcttggggaa cagcaacgct
     4081 cgacagcctt tgccgcgctg gaaggggcgc cattctccct cgggctgggc gagccggtcg
     4141 gcaaggacgt gaaagacgag cgggttgtgg gccatgcccg aatggctgcc cagcacctcg
     4201 acgttctcgc tctgctcgcc gtccagctcc aggcagctcg accagtggac cacgccgtca
     4261 gacttgctgt agatggcggt ggtgggtacc gagagagagg cttcggg
//
LOCUS       DBNHQR010000518         1321 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_747031, whole genome shotgun sequence.
ACCESSION   DBNHQR010000518 DBNHQR010000000
VERSION     DBNHQR010000518.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_747031"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..>1321)
                     /locus_tag="AC16VR_11845"
     CDS             complement(129..>1321)
                     /locus_tag="AC16VR_11845"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6011892.1"
                     /translation="AGMFLALLLARRGVTVTVLELHRDLDRDFRGDTVHASTLEVLDQ
                     IGLADRLLALPHAKMRQVTLHTAARTLELVDFSRLKTRFPYVMVMPQVVFLNYLHEQA
                     KSYPGFRCVLGAQVQQLVEVDGRVSGVRYRRDGEEHELRADLVIAADGRFSRLRKLAG
                     LEAESLSPPMDVCWFRLPREASDGHEAGGFFVGQGRMLIALPRSDEWQIGYVFPKGDF
                     KTLRESGIQAFRDSIAATAPWLAGRTDVIHGWERVHLLTVSSDRLPTWHRPGLLCIGD
                     AAHVMSPVGGVGINAAIGDAVEAANVLTRPLLDAVAPGEAALAEVQRRREVPTRIIQG
                     LQARIQDTVVSRALRDEEFHLPLPARLILATPGLRNLPPRIFAQGIKPLRLEALPRTE
                     HQRT"
BASE COUNT          228 a          438 c          445 g          210 t
ORIGIN      
        1 ctcgacgctg ccgccaccga ggtcgcccgt gaagaacagc aggtcgtagc gcgtcgcgtt
       61 gaactgcgcc acgtcgatat cgtcccagtg ggtgtgcagg tcgccgacca cgcccaaacg
      121 cagccccgtc atgttctttg atgctccgtg cgggggaggg cttccaatct tagcggcttg
      181 atgccctgcg cgaaaatcct tgggggaagg tttctcagcc ccggcgtggc gaggatcagc
      241 cgggcgggaa gcggcagatg gaactcctcg tctctcagtg cgcgcgacac cacggtgtcc
      301 tgtatgcgcg cctgcaagcc ctgaatgatc cgggtgggaa cctcccggcg ccgctgaacc
      361 tcggccaggg ccgcttcgcc gggagcgacg gcgtccagca ggggtcgagt cagaacgttc
      421 gctgcctcga cggcgtcgcc aatggccgca ttgatgccca caccccccac cggggacatg
      481 acgtgcgcgg cgtccccgat gcagaggagc ccgggccggt gccacgtcgg cagccggtca
      541 gagctcaccg tcaacaggtg caccctttcc cagccgtgaa tgacgtcggt gcgtccggcc
      601 agccagggcg cggttgccgc gatggagtcc cggaacgcct ggattccgga ttccctgagc
      661 gtcttgaagt ctcccttggg aaacacgtag ccgatctgcc actcgtccga acggggcagc
      721 gcgatgagca tgcggccttg cccgacgaag aaaccgcccg cctcgtggcc gtcggatgct
      781 tcccggggca gccggaacca gcagacgtcc atgggcgggc tcaggctttc ggcctccagc
      841 ccggccagct tgcgcagccg cgagaagcgc ccgtcggcgg cgatgaccaa atcggcgcgc
      901 agctcgtgct cctcgccgtc gcgccgatag cggacgcccg agacgcgtcc gtccacctca
      961 acgagctgtt gaacctgggc gccgagaacg caccgaaatc ccgggtagct tttcgcctgc
     1021 tcgtgaaggt agttgaggaa gacgacttga ggcatgacca tcacgtaggg aaagcgcgtc
     1081 ttcagccgcg aaaagtccac cagctcgagc gtccgcgcgg cggtgtggag agtgacctgg
     1141 cgcatctttg cgtggggcag cgcgagcagg cggtcggcca ggccgatctg atcgagcacc
     1201 tccagcgtgg aggcatgcac ggtatctccg cggaagtcgc ggtccaggtc gcgatggagc
     1261 tcgaggacgg tgaccgtcac tcctcgccgg gccaaaagaa gtgccaggaa cattcctgcg
     1321 g
//
LOCUS       DBNHQR010000519         2595 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_873564, whole genome shotgun sequence.
ACCESSION   DBNHQR010000519 DBNHQR010000000
VERSION     DBNHQR010000519.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_873564"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="AC16VR_11850"
     CDS             complement(<1..556)
                     /locus_tag="AC16VR_11850"
                     /inference="COORDINATES: protein motif:HMM:NF028503.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbiEi antitoxin N-terminal domain-containing
                     protein"
                     /protein_id="HIR6011893.1"
                     /translation="MSSKINQLLQQWPPRTVGTLRWLSRKGVDRRLADKYVQSGWLER
                     LGHGAYRRTGATVDWPGAVHALQSQLGLDLHPGGITAIELRGYSHYVAFAGRAVVLFG
                     KPGTKLPAWFVDHSWSRPVTLVTTGVFSNGDKATSSLQFDEVELDVATLEQAAFEVMY
                     LIPKRQSYEEAVQVMESLTSLRPQV"
     gene            complement(780..1520)
                     /locus_tag="AC16VR_11855"
     CDS             complement(780..1520)
                     /locus_tag="AC16VR_11855"
                     /inference="COORDINATES: protein motif:HMM:NF012920.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HIR6011894.1"
                     /translation="MPSRFSRFIFTIVLNTNPADAMKRRHDTVRLSNLELLVAEAGSA
                     AALARLAGTSESYLSQIRNQLLTAKGTPRGVGDELAAKLEQAMGKPHGWMDESHQEFM
                     EERARYNAEPGPDLRSLHPLISWVQAGGWAEIAGGFALGDAEDLLPCPVRCSPDTFVL
                     RVRGESMEPKFHDGDLIFVDPEAAPLSGRYVVVRLEDSHEATFKQLIVEGDRQFLKAV
                     NPDWPQRIIEVDSNATICGVVVFKGEVV"
     gene            1551..1826
                     /locus_tag="AC16VR_11860"
     CDS             1551..1826
                     /locus_tag="AC16VR_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011895.1"
                     /translation="MELKYFLKAASKRERAEVATVCNDSVSYLYQLAGKHRYASALMA
                     ARIETQTRAVAAGTDGRLATVPRETLVRYPEIFERDEPYPMVAEERP"
     gene            1823..>2595
                     /locus_tag="AC16VR_11865"
     CDS             1823..>2595
                     /locus_tag="AC16VR_11865"
                     /inference="COORDINATES: protein motif:HMM:TIGR03764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HIR6011896.1"
                     /translation="MTGKAALRQSGTGQSPGDGLELVYLDVARIKPYEHNPRRAPNAE
                     FDRIKASIRVDGLGQPLVVTQRPGESDYLVHAGGNTRLRILKELFAETEDPRFSRVAC
                     VVRRWTREADVLLAHLKENDLRGELTFLDKALAVANAKRLLEEDGSAEFTQARLAEVL
                     TRSGYGLSQGLISQMAYVVERLRPLLPQTLESGIGRPQVARIRALDRAARSLWLERPV
                     DTEAEYDQVFEILCCRYDTPDWDIAHLRRALEAEIAERA"
BASE COUNT          489 a          849 c          808 g          449 t
ORIGIN      
        1 cgacctgagg acgcagtgac gtcagcgatt ccatgacctg gacggcttcc tcgtacgact
       61 gccgcttcgg tatgaggtac atcacttcga aggctgcttg ctcgagagtc gcaacatcga
      121 gctcgacctc gtcgaactga agcgacgagg tggccttatc gccgttggag aacacgccag
      181 tcgtaaccag cgtcaccggc cgggaccagg agtgatccac gaaccacgcc ggcagcttcg
      241 tgccaggctt gccgaacagg acgaccgcgc gtcctgcgaa cgccacgtag tgggagtaac
      301 cgcgcagctc gatggcggtg atcccgccgg ggtgcaggtc aagaccgagc tggctctgca
      361 gcgcgtgaac ggcgcccggc cagtcgaccg tcgccccggt tcgcctatag gcgccatggc
      421 ccaatcgttc cagccagccg gactggacgt acttgtccgc aagccgacgg tcgacgccct
      481 tgcggctcaa ccagcgtagg gtgcctaccg ttcgaggtgg ccattgctgc aacaactggt
      541 ttattttcga gctcatgcga caaccaatcg gcgacgttca cgcgtcagaa taaactgatg
      601 gacgaggtta gcaccaagtt cgggaaaatt ccaaaatgtc gccgctaggg cgacaattcc
      661 gcctgaaacc gaactgggct ccgccaccca gacatctgcg accggctgtc actccgggcg
      721 cgaacaggcc gaatcgctct ccaagccttg gtgccgcgca aagagcgcga taggcggtgc
      781 taaaccacct cccccttgaa cacgaccacc ccacagatcg tcgcattcga atccacctct
      841 atgatccgct gcggccagtc ggggttcaca gccttcagga actgccggtc gccctcgacg
      901 atcagctgct tgaaggtcgc ttcgtgcgaa tcttccaggc gaacgacgac gtaccggccg
      961 ctgagtggcg cagcctcggg atcgacgaag atcaggtcgc cgtcgtggaa cttgggctcc
     1021 atgctttcgc cccggacgcg cagcacgaag gtgtccggac tgcagcgaac cggacagggg
     1081 agcaggtctt cggcgtcgcc cagcgcgaag ccgccggcga tttcggccca tccgcccgcc
     1141 tgaacccacg agatcagtgg atgcaagctg cgcaggtccg ggccgggctc agcgttgtag
     1201 cgtgcgcgtt cttccataaa ttcctggtgc gactcgtcca tccagccgtg cggcttgccc
     1261 atggcctgct cgagcttcgc tgccaattca tcacccacgc cgcgcggcgt gcccttcgcg
     1321 gtcagtagct ggttgcgaat ctgactgagg taagactcgc tggtacccgc gagacgggca
     1381 agcgcggctg ccgatccagc ttcggcaacc aggagctcga ggttgctcag ccgaaccgtg
     1441 tcatgccttc gcttcatcgc atccgccggg ttcgtgttta gcacgatggt aaagataaac
     1501 ctgctaaatc tgcttggcaa gctaaactat ctcgcgctac cgtcttgtgc atggagctaa
     1561 agtattttct gaaagcggca agcaagcgcg agcgggcgga agtggccacc gtgtgcaacg
     1621 attcggtgtc ctatctctac cagctggctg gcaagcaccg ttacgccagc gcgctgatgg
     1681 ccgcccggat cgagacccag acccgtgcgg tggcggccgg gacagatggg cgtctggcga
     1741 ccgtccctcg ggagactctt gttcggtacc cggagatttt cgagcgtgat gagccgtacc
     1801 ccatggtcgc cgaggagcga ccgtgaccgg gaaggcggcc ctgcgccaga gcgggaccgg
     1861 ccaatccccc ggggacggcc tcgagctggt ctatctggac gtggccagga tcaagcccta
     1921 cgagcacaac ccgcgccgag cgccgaacgc ggaattcgac cgcatcaagg catccatccg
     1981 cgtcgacggg ctcggtcagc cgctcgtcgt tacccagcgc ccgggtgaat ccgactatct
     2041 ggttcacgcg ggcggtaaca cccgcctgcg catactgaag gagctcttcg ccgagacgga
     2101 agacccccga tttagcaggg tcgcctgcgt ggtacgacgc tggacgcggg aggccgatgt
     2161 cctgctcgcg cacctgaagg agaacgatct gcgtggcgag ctcacctttc tggacaaggc
     2221 cctcgcggtg gccaacgcca aacgtctcct ggaggaagac ggctcggccg agttcaccca
     2281 ggcacggctc gccgaggttc tcacacgatc cggctatggt ctgagccagg ggctcatctc
     2341 gcagatggcg tacgtggtgg agcggctacg accgttgttg ccgcagacct tggagagcgg
     2401 catcggccgg ccccaggtcg ccaggatccg cgcactggat cgcgccgccc ggtccctatg
     2461 gctggagcgt cccgtcgata ccgaggccga gtacgaccag gtcttcgaga tcctctgctg
     2521 ccgctacgac acacccgact gggacatcgc ccatctgcgc cgcgcgctgg aggcagaaat
     2581 cgccgagcga gcgga
//
LOCUS       DBNHQR010000520         3502 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_304286, whole genome shotgun sequence.
ACCESSION   DBNHQR010000520 DBNHQR010000000
VERSION     DBNHQR010000520.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_304286"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="AC16VR_11870"
     CDS             <1..296
                     /locus_tag="AC16VR_11870"
                     /inference="COORDINATES: protein motif:HMM:NF013998.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR6011897.1"
                     /translation="MGDHWRDALKGLSRHNEVVAVRIFDPLERDLPPPGRYTVTDTEQ
                     RWQFHSGNRLLRKTYRERFADHEIELKTICEGSGVRYRSLATDARVGPMAEWA"
     gene            296..772
                     /locus_tag="AC16VR_11875"
     CDS             296..772
                     /locus_tag="AC16VR_11875"
                     /inference="COORDINATES: protein motif:HMM:NF025674.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4381 domain-containing protein"
                     /protein_id="HIR6011898.1"
                     /translation="MQPNPLEQLRDIHLPPDPGWWPPAIGWWLLALVIAFVCAAGIRA
                     IYEYRRRRRPVRQARRLYERLSEHLRSGSLSGEAFTHECNELLKRLLVHGFGERDARA
                     ATDETWLAMLDERYGERAFTTGPGRLLGNERFRPDPEFATDELDALMQRFLKRVQP"
     gene            769..1770
                     /locus_tag="AC16VR_11880"
     CDS             769..1770
                     /locus_tag="AC16VR_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012075094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR6011899.1"
                     /translation="MIEWLWPWAFLLAPLPLAMRLLVSPVARQQAALTVPSVAAFRAT
                     DDSAGTASGRFPWRAVALWLIWLALVTATARPQWTGDPVTLPVTGRDLMLAVDISGSM
                     GTEDLELGGRMVNRLTVVKNVVSQFVDARRGDRVGLILFGTNAYLQAPLTFDLDSVNR
                     LLVEAPVGIAGGKTAIGDAIGLAVKRLRRRPEGERVLILLTDGASNVGEVAPIKAAEL
                     ARQEEIRIHTIGVGADEMRMPSLFGVFGSRVINPSAELDEETLQTIADATGGRYFRAQ
                     NTQRLQEIYTIIDALEPVEQDAETYRPIAALYYWPLGAAWFMSMLLIAGNWRGWGRD"
     gene            1763..3478
                     /locus_tag="AC16VR_11885"
     CDS             1763..3478
                     /locus_tag="AC16VR_11885"
                     /inference="COORDINATES: protein motif:HMM:NF024909.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR6011900.1"
                     /translation="MIESLQFIRPWWLLGLIPAIGLAALWARNRAAGSHWESIIAPEL
                     LNVLLEPTGKGGMRRTAVLAALALAAATVGLAGPTWERLPQPVEQRSDALVVVLDLSL
                     SMYAQDVPPSRIVRARQKITDVLRARDEGFTGLVAYAGDAHSVAPLTDDTETVQNLLA
                     ALSPGMMPVLGSNARSAIDLARQLLDNAGFAQGRILLVTDGVARASDLTDQAHPSYPI
                     SILGVGTASGGTIPLDFVDQPGDVLRTESGEIVRARLDAQYLAALADACHGRYRSMSL
                     GDEDIAFLTATPLPQDAEMLEVEREFDTWADMGYWVCIALIPLALLGFRRGLLAAVCI
                     GILPLPAEANLWDDLWQRRDQQAVEMLEQGDPAAAANLFEDPDWRAAARYRAGDYPGA
                     AEAFAAQLSATGIYNLGNALAHQGDLQTAIAAYSEVLAQQPGHEDAAFNKALLEELLE
                     QQQSQQQQDQNQQDDGQGSEDDQSTPSGSGEQQDQTAEQDPGEQSDRPSGQSASGDEP
                     SEPAPGSETELADARDEQQDALEQWLRRVPDDPGGLLRRKFRYETNQRLRRGEYQSNP
                     SEQIW"
BASE COUNT          585 a         1168 c         1176 g          573 t
ORIGIN      
        1 ccatgggcga tcactggcgc gatgcgctca aggggctgtc gcgccacaac gaggtggtcg
       61 ccgtccggat attcgacccg ctggagcgag acctgccgcc accaggccgc tatacggtga
      121 ccgataccga gcagcgctgg cagtttcaca gcggcaaccg gctgcttcgc aagacctatc
      181 gggaacgctt cgcggatcac gagatcgaac tgaagaccat ctgcgagggc tccggcgtcc
      241 gctaccgttc gctggcgacc gacgcgcggg tggggccaat ggccgagtgg gcgtgatgca
      301 gccgaatccc ctggagcagc tcagagacat ccatctgccc ccggaccccg ggtggtggcc
      361 gccggccatc ggttggtggc tgctggcatt ggtgatcgct tttgtctgcg cggccggcat
      421 ccgggccatc tacgagtatc ggcgccggcg ccgtcccgtc cggcaggccc ggcgtctcta
      481 cgagcgtctc agcgagcacc tcaggtcggg gtccctttcc ggcgaggcct tcacccacga
      541 gtgcaacgag cttttgaagc ggttgctcgt tcacggcttc ggcgagcgtg acgccagagc
      601 ggccacggac gaaacctggc tggcgatgct ggacgaacgt tacggcgaac gggcgttcac
      661 caccggtccg ggccggcttc tcggcaatga gcgctttcgc cccgacccgg agtttgccac
      721 cgacgaactc gacgcgctga tgcagcgctt ccttaagcgg gtgcagccat gatcgagtgg
      781 ctgtggccgt gggccttcct cttggcgcca ctgcccctgg caatgcggct gctcgtcagc
      841 cccgtggcgc gtcagcaggc agctctaacg gtgccaagcg tcgcagcttt ccgcgccacg
      901 gacgatagcg ccggcacggc cagcggacgc tttccctggc gcgcggtcgc gctgtggctg
      961 atctggttgg cgctggtcac ggccaccgca aggccgcaat ggacgggcga tccggtcacg
     1021 cttccggtaa ccggaaggga cctcatgctg gccgtggaca tatccggaag catgggcacc
     1081 gaggatctgg agctgggcgg gcgaatggtc aatcgcctga cggtggtcaa gaacgtcgtc
     1141 agccagttcg tggacgcccg gcgcggtgac cgcgtcgggc tgatcctgtt cggcaccaat
     1201 gcctacctcc aggcgccgct cacgttcgac ctggacagcg tcaaccgcct gctggtggaa
     1261 gctcccgtgg gcatcgccgg cggcaagacg gcgatcggtg acgccatcgg tctggccgtc
     1321 aaacggctgc gcagaagacc cgagggcgaa cgcgtgctca tccttcttac cgatggggcg
     1381 agcaacgtcg gcgaggtcgc gccgataaag gcggcagagc tcgctcgcca ggaggagatc
     1441 cgcatccaca ccatcggcgt gggcgcggac gagatgcgga tgccgagcct tttcggcgtc
     1501 tttgggtcca gggtgatcaa cccctcggcg gagctcgacg aggaaaccct gcagaccatc
     1561 gccgacgcca ccggagggcg ctacttccgc gcccagaaca cccagcgcct tcaggaaatc
     1621 tacaccatca tcgacgcgct cgagcccgtg gagcaggatg ccgagaccta ccggcccatc
     1681 gccgcgctct actactggcc gctgggcgcc gcctggttca tgtcgatgct gctgatcgcc
     1741 ggcaactggc gaggctgggg ccgtgattga gtcattgcaa ttcatccgcc cgtggtggct
     1801 gcttggcctg attcccgcca ttggcctggc tgcgctgtgg gcccgaaacc gcgccgcggg
     1861 gtcgcactgg gagtccatca tcgccccgga gcttctcaac gttctgctcg agccaaccgg
     1921 caaggggggc atgcggcgaa cggccgtcct cgcggcattg gcgctggcgg cagcaaccgt
     1981 cggtctggcg gggccgacgt gggaacgcct gcctcaacct gtggagcaga gaagcgacgc
     2041 tctggtggtc gtgctcgatc tctcgctgtc catgtacgcg caggatgtgc cgccctcgcg
     2101 catcgttcgc gctcgccaga agatcacaga cgtccttcgg gcccgggacg agggtttcac
     2161 cggcctggtc gcctacgcgg gagacgctca ctcggttgca ccgctgaccg atgacaccga
     2221 gaccgtccag aacctgctgg cagctttgag cccgggcatg atgcccgtcc tcggcagcaa
     2281 cgcccggtcg gccatcgacc ttgcgcggca gctcctggac aatgccggtt ttgcgcaggg
     2341 acggatcctg ctggtcaccg acggcgtcgc gcgcgcctcg gatctcaccg accaagctca
     2401 ccccagctat cccatctcca tactcggcgt gggcaccgcc tcgggcggca cgatccccct
     2461 ggatttcgtt gatcagccgg gagacgtgct gcgaaccgaa tcgggagaga tcgtccgcgc
     2521 ccgcctggat gcccagtatc ttgccgccct tgccgacgcc tgccacggcc gttatcggtc
     2581 catgagtttg ggcgacgagg acattgcctt tctcacggcg accccgctgc cccaggacgc
     2641 cgaaatgctt gaggtggaac gggagttcga cacgtgggcg gatatgggtt actgggtctg
     2701 catcgcgctc atcccgctcg cgctgctcgg tttccggcgc ggtctgctag ccgccgtgtg
     2761 cattgggatc ctcccgctgc ccgccgaggc aaacctctgg gacgatttgt ggcaacggcg
     2821 ggatcagcag gccgtggaga tgctcgagca gggcgacccg gcggccgctg cgaacctgtt
     2881 cgaggatccc gactggcgcg ccgccgcccg ataccgggca ggcgactacc caggggcggc
     2941 ggaggcgttt gccgcccagc tgtcggcaac gggcatctac aacctgggca acgccctggc
     3001 gcaccagggg gatctgcaga ccgccatcgc cgcctacagc gaggtgctgg cgcagcagcc
     3061 cggccatgaa gatgcggcct tcaataaagc cctcctcgag gagttgctgg agcagcagca
     3121 atcgcaacag cagcaggatc agaaccagca agacgacggg caaggatcag aagatgacca
     3181 gtccacgccc tccggttccg gtgagcagca ggatcagacc gcagagcagg atcctgggga
     3241 gcagagcgat cggccttcgg ggcaatcggc gtccggcgac gagcccagcg agccggctcc
     3301 ggggtcggag acggaactgg ctgacgctcg cgatgagcag caggatgcgc tggagcagtg
     3361 gctgcgccgg gtgcccgacg atccgggcgg ccttctgcgc cggaagttcc gctacgaaac
     3421 caatcagcga ctgcgcagag gcgagtacca aagcaacccc tcggagcaga tatggtgagc
     3481 cggcgactca tcgtcctgct ca
//
LOCUS       DBNHQR010000521         4831 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_874330, whole genome shotgun sequence.
ACCESSION   DBNHQR010000521 DBNHQR010000000
VERSION     DBNHQR010000521.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4831)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4831
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_874330"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="AC16VR_11890"
     CDS             complement(<1..247)
                     /locus_tag="AC16VR_11890"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017130527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR6011901.1"
                     /translation="MTSAHGQLTSALSPSQVRTDPDSLHEWGRDWTRTAEPAPAAVVF
                     PETTADVVAIVRLANSEGLKLVPSGGRTGLSGGAVAAD"
     gene            complement(255..1214)
                     /locus_tag="AC16VR_11895"
     CDS             complement(255..1214)
                     /locus_tag="AC16VR_11895"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023568.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6011902.1"
                     /translation="MEVGFDPDSLRQALKPLVPGSPTVLTPQEDAYLRHYGIHFVEEY
                     PDLAHHFGAVETDSHSVAAHLWSPRDARGTAVIVHGYYDHTGLYGHLIRHLIDRGLAV
                     LSFDLPGHGLSTGAPATIETFDHYVAAFDACIAALGAHLPKPWHLLGQSTGGAVAMEW
                     LLANGVARATSPFSGIVLLAPLVRPYLWPVGRLFYEVVRRFITERERTFSVNAENPEF
                     IEFLKHHDPLQARVLPVQWVTAMVAWKKRFVSRAPSDLGLLVVQGTADRTVDHRYNLK
                     IIEQLFDAKVVYVPEARHHLVNESVVLRQQIFQAVDAQLDAAG"
     gene            complement(1228..2391)
                     /locus_tag="AC16VR_11900"
     CDS             complement(1228..2391)
                     /locus_tag="AC16VR_11900"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIR6011903.1"
                     /translation="MSQRRNMTDLDLAERRVLIREDLNVPIRDGRITSDARVRAALPT
                     IEAAMRAGARTLVMSHLGRPVEGEFDPALSLAPVAGRLGELLGVDVPLVGLDDVGVID
                     PGAVSLLENVRFLKGEGADDEALARRLASLCDIYVMDAFATAHRAQASTHGVAKFAPV
                     ACAGPLLAGELEALERALRAPARPMTAIVGGAKVSTKLEVLDTLATIADRIIVGGGIA
                     NTFIAAAGHPVGRSLYEEDLLEAARSIAAKVEVPLPVDVMVGERLDAGAVAVLRGLDE
                     VRPDEMILDIGPETARRYAAELGSAGTILWNGPLGVFEFDQFGEGTRVVAQAIASSGA
                     FSIAGGGDTLAAIDKYDVRDGVSYISTGGGAFLEFVEGKTLPAVAVLASRAEG"
     gene            complement(2399..4381)
                     /gene="tkt"
                     /locus_tag="AC16VR_11905"
     CDS             complement(2399..4381)
                     /gene="tkt"
                     /locus_tag="AC16VR_11905"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007155654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIR6011904.1"
                     /translation="MVTRTDRANAIRALAMDAVQAANSGHPGAPMGLADIAEVLWRDF
                     LKHNPADPAWANRDRFVLSNGHGSMLLYSVLHLSGYAVTMDDLRSFRQLHSRTPGHPE
                     YSECPGVETTTGPLGQGLANAVGMALAEERLSRRFNRDGFSVVDHRTWVIVGDGCLME
                     GISHEAASLAGTLQLGKLICIYDDNGISIDGDVKAWFTEDVPARFEAYGWRVIRDVDG
                     HDPNSIQRALDDAVSRQDRPTLVCCRTVIGFGAPNKQGTADVHGSALGEEEVANARRE
                     LGWAAPPFEIPEPVYASWDCREKGAEQQRSWEALLAEYRAAHPEDAAELERRVSGDLP
                     EGWEGPIRALAEASQEAMEPLETRKSSQRCLAALAGALPELFGGSADLTGSNGTRWDG
                     ADEDRYLSFGVREFGMTAITNGMALHGGFRPFSGTFLVFMEYARNAVRLAALMKIPNI
                     FVYTHDSVAVGEDGPTHQPIEQLTNLRTTPGLSTWRPCDTVESAVAWEAAVASRQRPT
                     ALIFTRQKTAPQPRSAQAFADIRRGGYVLKAEAGTLAAVVIATGSEVEIAVAAAASLE
                     SDGVGVRVVSMPSTDVFLSQDRAYQDAVLPPALRARVAVEAAHPDYWYRFVGLDGAVV
                     GIDRFGLSAPGGQAMAALGITADNVAEAVRSVLKEA"
     gene            4612..>4831
                     /locus_tag="AC16VR_11910"
     CDS             4612..>4831
                     /locus_tag="AC16VR_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6011905.1"
                     /translation="MNEYSIFTSESVGEGHPDKMADQISDAVLDAMLEQDAESRVACE
                     TLIKNNLVVLAGEIRTDAWVDVDRLARQV"
BASE COUNT          857 a         1660 c         1586 g          728 t
ORIGIN      
        1 cgtccgcggc caccgcaccc ccggaaagac ccgtgcgccc acccgacggc accagcttca
       61 ggccctcgga gttggccagg cggacgatgg cgaccacgtc agcggtagtc tcgggaaaaa
      121 cgacggcggc aggcgcaggc tcggcagtcc gggtccagtc ccgcccccac tcgtgcaggc
      181 tgtcggggtc agtgcggacc tgcgaaggcg atagcgcgga ggtcagctgc ccgtgcgcag
      241 atgtcatccc ccgctcatcc ggcggcatcg agctgcgcat ccaccgcctg aaaaatctgt
      301 tggcggagga ccaccgactc gttgaccagg tggtgtcgcg cctcggggac atagaccacc
      361 ttggcatcga agagctgctc gatgatcttc aggttgtagc gatggtcgac cgtgcggtcg
      421 gcggtgccct gcaccaccag cagccccaga tcgcttggcg cgcggctgac gaagcgcttc
      481 ttccaagcca ccatggcggt gacccactga acgggcagca cgcgcgcctg cagcgggtcg
      541 tggtgcttga ggaactcgat gaactccgga ttctcggcgt tgacggagaa cgttcgctcc
      601 cgctcggtga tgaagcgccg cacgacctcg tagaagagcc ggccgacggg ccacaggtac
      661 ggccgcacca gcggtgctag gagcacgatg cccgaaaacg ggctggtcgc ccgagcgacg
      721 ccgttggcca ggagccactc catggccacc gcgcctcccg tgctctggcc cagcaggtgc
      781 catggcttgg ggaggtgcgc gccgagcgcc gcgatgcagg cgtcgaacgc ggcgacgtag
      841 tgatcaaacg tttcgatggt ggccggcgcg ccggtggata gcccgtgccc gggcaggtcg
      901 aacgacagca cggcaagccc gcggtcgatg aggtggcgga tcaggtgccc gtaaaggccc
      961 gtgtggtcgt agtagccgtg cacgatcacg gcggtgcccc gggcatcgcg cggcgaccag
     1021 agatgggcgg cgacgctgtg cgaatccgtt tccaccgccc cgaaatgatg ggccaggtcg
     1081 ggatactcct cgacgaagtg aatgccgtag tgccgcaggt aagcatcctc ctgcggcgtc
     1141 agcaccgtgg gagagccggg gacgaggggc ttaagcgcct gcctgaggct gtccgggtcg
     1201 aaaccgactt ccatcgaccg ctgaccgtta accttcagcc cggctcgcga ggacggccac
     1261 ggcgggcagg gtcttgccct ccacgaactc gaggaacgcg cctcccccgg tggagatgta
     1321 ggacacccca tcgcgcacgt cgtacttgtc gatggctgcc agggtatccc cgccgccggc
     1381 tatggagaac gcaccgctgg acgcgatggc ctgggcaacc acgcgagtgc cctcgccgaa
     1441 ctggtcgaac tcgaagacgc ccagcgggcc gttccagaga atggtgcccg cagacccaag
     1501 ctctgccgcg taacgtcggg ccgtctccgg accgatgtcc aggatcatct cgtcggggcg
     1561 gacctcgtcg agcccccgca gcaccgccac cgcgcccgcg tccaggcgct cgcccaccat
     1621 cacgtccacc ggcagcggca cctccacctt ggcggcaatg ctgcgcgccg cttccagcag
     1681 gtcctcctcg tagagcgagc gccccacggg gtgaccggcg gcagcgatga aggtattggc
     1741 tatgccgccg ccaacgatga tgcggtcggc aatggtcgcc aaggtgtcca ggacttcgag
     1801 cttggtcgaa accttcgcac ccccgacgat ggccgtcatg ggccgggcgg gcgccctcaa
     1861 ggcgcgctcc aacgcctcga gctcaccggc cagcaacggt ccggcgcagg ccaccggggc
     1921 gaacttcgca acgccgtggg tggaagcctg tgcgcgatgg gcggtggcaa acgcatccat
     1981 gacgtagatg tcgcacagcg acgccagccg cctggcgagg gcttcgtcat cggcaccctc
     2041 gccctttaga aagcgaacgt tctccagcag cgagacggcg ccggggtcga tcacgcccac
     2101 gtcgtccagg ccgaccaatg ggacatccac gcccagcaac tcgcccagcc gccccgccac
     2161 gggggccaag gacaacgccg ggtcgaactc gccctccacc gggcggccga ggtgagacat
     2221 caccagggtt ctcgcgccgg cgcgcatggc ggcctcgatg gtcggcagcg ccgcccggac
     2281 ccgcgcgtcg ctcgtgatac ggccatcgcg gatcggcacg ttcagatcct cccggatcag
     2341 cacccgccgc tccgcgagat ccagatccgt catgttcctt cgctgcgaca tgaaaagatc
     2401 aggcctcctt cagaacgctc cggacggcct cggcgacgtt gtccgcggtg atgccgaggg
     2461 cggccatggc ctggccaccc ggcgcggaga gaccgaagcg gtcgatgccc acgacggcgc
     2521 cgtccaggcc cacgaatcga taccagtagt caggatgcgc ggcctccacc gccacccgcg
     2581 ctcgcagcgc agggggcaat acggcgtcct ggtaggcacg gtcctgggac aagaaaacgt
     2641 cggtggaggg catggagacc acccgcaccc cgacgccgtc cgactcgagg ctcgctgccg
     2701 ccgccacggc gatctccacc tcggaccccg tggcgatgac cacggcggcc agcgtgccgg
     2761 cctcggcttt caggacataa cctccccggc ggatgtcggc gaacgcctga gcagaacggg
     2821 gctggggcgc cgtcttctgg cgggtgaata tcagcgcggt cggtcgctgc cgggacgcca
     2881 ccgccgcttc ccaggcaacc gcactctcca cggtgtcgca agggcgccac gtcgacaatc
     2941 cgggcgtggt ccgaagattg gtgagctgct cgatgggctg atgggtcggg ccgtcctcac
     3001 cgacggccac ggagtcatgg gtgtacacga agatgttggg gatcttcatc agcgccgcaa
     3061 gccgcacggc gttgcgggca tactccatga acaccagaaa agttccggag aacggtcgaa
     3121 agcccccgtg gagggccatg ccgttggtga tggccgtcat gccgaactcg cgaacgccga
     3181 agctcaaata ccggtcttcg tctgcaccat cccagcgggt cccgttggag cccgtgagat
     3241 cggcggagcc tccgaaaagc tccggcaggg cgccggccag cgcggcaagg catcgctggg
     3301 acgatttccg cgtctccagg ggctccatcg cctcctggga cgcttcggcc agggcgcgga
     3361 tcggaccctc ccaaccttcc ggcagatcgc cggacacgcg gcgttcgagc tccgccgcat
     3421 cctccgggtg cgcggcgcga tactccgcca gcagcgcctc ccaggaccgc tgctgctcgg
     3481 cgcctttctc ccgacagtcc caggaggcgt agacgggctc cggaatctcg aagggcggcg
     3541 cagcccagcc gagctcccgg cgggcgtttg ccacctcttc ttcgcccagc gccgagccgt
     3601 gcacgtcggc agttccctgc ttgttgggcg cgccaaaacc gatcaccgtc ctgcagcaaa
     3661 ccagggttgg tcgatcctgc cggctcaccg cgtcgtccag agcccgctga atcgaattcg
     3721 ggtcgtgccc gtccacgtcg cgtatgacgc gccaaccata ggcctcaaag cgagccggga
     3781 cgtcctccgt gaaccaggct ttgacgtccc catcgatgga gatgccgttg tcgtcgtaga
     3841 tgcagatgag cttgccgagc tgcagggtgc cggcaagcga agccgcctcg tgagagatcc
     3901 cctccatgag gcagccgtca ccgacaatca cccaggtccg gtgatctacc accgagaaac
     3961 cgtcgcggtt gaagcgccgg gagagccgct cttcggccag cgccattccc actgcgttag
     4021 ccaggccctg gcccagcgga ccggtggtcg tttcgacacc cggacattcg ctgtactccg
     4081 ggtgaccggg cgtgcgcgag tgaagctgcc tgaacgagcg cagatcgtcc atggtgaccg
     4141 cataccccga cagatgcagc acgctgtaca gcagcatgga tccgtgaccg ttggaaagga
     4201 cgaaccgatc gcggttggcc caggccggat ccgccgggtt gtgcttgaga aagtcgcgcc
     4261 acagcacttc tgcaatgtcg gccagaccca tgggcgcccc gggatgaccg gagttagcgg
     4321 cctgcaccgc gtccatggcg agcgcacgaa tggcgttcgc cctgtcagtc ctcgttacca
     4381 tttggttttc gctagcgatg taagagtaag gatcggtgcc agccccctgc cgaccctgtc
     4441 tattgtccgt tactcgcacc gacatgcgca aatgccgcgc gccccatcgg gcactggccg
     4501 cctgggctcg gaaaggccgc gccgtgagtc gaaccagaga aaagctgtac aatgcgccgc
     4561 ttgccgcaac agggcctggc agggcttgga agagaaccgg aaggaagaaa catgaacgaa
     4621 tacagcatat tcacctcgga gtcggtgggc gaggggcacc ccgacaagat ggcggatcaa
     4681 atctccgatg cggtgctcga cgccatgctc gaacaggatg cagagtcccg cgtcgcctgc
     4741 gagacgctga tcaagaacaa tctggtcgtt ctcgccggcg agatccgcac cgatgcatgg
     4801 gtcgacgtcg accggctggc ccgtcaggtc a
//
LOCUS       DBNHQR010000522         4598 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_241154, whole genome shotgun sequence.
ACCESSION   DBNHQR010000522 DBNHQR010000000
VERSION     DBNHQR010000522.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4598)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4598)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4598
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_241154"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..1240
                     /locus_tag="AC16VR_11915"
     CDS             113..1240
                     /locus_tag="AC16VR_11915"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HIR6011906.1"
                     /translation="MLIGVPKETWPGEHRAALVPQSVKKLVGAGFEVVIEAGTGVPAG
                     YPDAAYVDAGATIGGDRAGLLASADVVLRVRKPEADEIAGLKKGSVHVSFLDPFNERG
                     LVDAMAAQGVTAISMEMIPRSTRAQKMDALSSQANLAGYVTVVLAANHCPKIFPMMMT
                     PAGTIAPSRVFVIGAGVAGLQAIATAKRLGARVEAFDTRPVVAEQVRSLGGKFVEIDL
                     GEVGQTEQGYAKELTPEQVELQKEGQKKVIGQSDVVITTAQVFGRPAPRIVSKDMVEA
                     MQPGSVVVDMAVETGGNVEGSVLNEVVDVGGVKIIGLGNLPSQVARHASDMYSSNLFN
                     LLDEFWSQEESKLDLDPEDEIVRACVITRDGSIVNETIKNL"
     gene            1253..1558
                     /locus_tag="AC16VR_11920"
     CDS             1253..1558
                     /locus_tag="AC16VR_11920"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011783921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha part 2"
                     /protein_id="HIR6011907.1"
                     /translation="MEAVFLAFVLMLSIFLGFELISKVPATLHTPLMSGANAISGITL
                     VGAITSAGAGADQIATLLGAVAVGFATINVVGGYLVTNRMLSMFKRKDTGGQSGGRS"
     gene            1555..2967
                     /locus_tag="AC16VR_11925"
     CDS             1555..2967
                     /locus_tag="AC16VR_11925"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011783920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HIR6011908.1"
                     /translation="MSLELINLWYIVAAALFIFGLKQLSSPDTAVRGNLLSSVGMLIA
                     VVVTLFSRDILEFQWIAAAAAVGAIIGAVAAQRVAMTSMPEMVALFNGSGGIASLLVG
                     WAALYLGDGAAAEAAVADTAVVTVGLSTFTIITILLSVLIGGLTFSGSLIAWGKLSEV
                     MTSRAIVFGAQRLVNAALLIGLVACGVMFAMEPSLTSPYLYAVIALSLIFGVMAVIPI
                     GGADMPVVISLLNSYSGLAACAAGFAINNNILIVAGSLVGAAGIILTNIMCKAMNRSL
                     ANVLFSGFGAVKTATKVEGEVKPITPDDAFLILEAASSVTFVPGYGMAVAQAQHVVRE
                     LGELLEENGAEVTYAIHPVAGRMPGHMNVLLAEANVPYDQLVEMDDINPRIDNIDVAV
                     VIGANDVVNPAAREDENSPIYGMPIINVDQARTVFVLKRSMASGFSGVDNPLFFGENT
                     RMLFGDAKASISSVIAEFKG"
     gene            complement(3091..>4598)
                     /locus_tag="AC16VR_11930"
     CDS             complement(3091..>4598)
                     /locus_tag="AC16VR_11930"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR6011909.1"
                     /translation="AASFYAQASGKTGIVNLHVAPGLGNGIGMIYGALKANSPLIITA
                     GQQDTRMRRREPLLAHDLVAMAEPVCKWSAEPRSADEIGPMLRRAFKIANEPPRGPVF
                     MSLPVDVMEQETSVGATTAGDLRYGTLADAEGIRRLARWLAGSANPAIVVGDDIAAEG
                     AHRALITLAVQTGAAVWQQGLRAQISFPNVHPNSKGRLPFEAGAIRRALAGHDLILLM
                     GGPFFEEIWFDAENALPEEARVVHVESSHARLALNLAPDLGLVGNLRTTIEHLVEEIA
                     EGAPDDFGAAAKARNDALAESHRAAGDAAADRIERQGDTSPMTPARALLEISRALPKG
                     AIVVDESITASLEVASRFDYAAPGDFFAGSGGGIGQGLAGALGVQVAQPNRRVLAISG
                     DGSAMYSIQALWSAVHHGLPIVFVILSNREYRILKHNLDIYRQRFDAGSNKPYPHMDL
                     TRPALGFPAMAEGMGMEGELVTRPEKIGSAIERAFAANAPYLVEVEISGKP"
BASE COUNT          771 a         1441 c         1504 g          882 t
ORIGIN      
        1 ttaacatgtg aattgttttc gcgacggggt ttggctaact tgctgcgctt tcctgcgccg
       61 catcttccgc tctcttcttc atgccggcgc gactgcgatt tggggggatt gcatgctcat
      121 tggggtaccc aaggaaacct ggccggggga acatcgtgcg gcgctcgttc ctcagagcgt
      181 caaaaagctc gtaggtgccg ggttcgaagt agtcatagaa gccgggacgg gagtccctgc
      241 gggttatccc gacgccgcct acgtcgatgc cggtgcgacc atcgggggcg atcgggcggg
      301 cctgctcgcc tcggcggacg tcgtcctgcg ggtgcgcaag ccggaagcgg acgagatcgc
      361 cgggctcaaa aagggatcgg tgcacgtcag ctttctggat cccttcaacg agagagggct
      421 ggtcgatgcc atggcggccc agggcgtcac ggccatttcc atggagatga tcccgcgcag
      481 cacgcgcgcg cagaagatgg acgccctgtc atcccaggcc aacctcgccg gatacgtcac
      541 ggtggttctc gccgccaatc attgccccaa gatctttccc atgatgatga cgccggccgg
      601 gacgattgcc ccgtcgcggg tgttcgtgat cggcgcgggg gtcgccggac tgcaggcgat
      661 cgccacggcg aagcggctcg gcgctagagt cgaagcattc gatacccgcc cggtggtggc
      721 cgagcaggtt cgctctctcg gcggcaagtt cgtcgagatc gatcttggcg aggtcgggca
      781 gacagagcag ggctatgcaa aagagctgac gcctgagcag gtggagctcc agaaagaagg
      841 tcagaaaaag gtgatcggcc agtccgacgt ggtcatcacc accgcgcagg tgtttggtcg
      901 tccggcaccc cgcatcgtgt caaaggacat ggtcgaggcc atgcagccgg gaagcgtcgt
      961 ggtcgacatg gccgtcgaga ccggcggcaa cgtcgagggc tctgttctca acgaagtggt
     1021 cgacgtcggc ggcgtgaaga tcattggcct gggcaatttg ccgtcgcagg ttgcgcggca
     1081 tgccagtgac atgtactcga gcaatctctt caacctgctg gatgaattct ggagccagga
     1141 ggaaagcaag ctcgacctcg atccggaaga cgaaatcgtt cgggcctgcg tgatcacgcg
     1201 ggacggatcg atcgtcaacg aaaccatcaa gaacctctag gagtccggaa ccgtggaagc
     1261 cgttttcctc gccttcgtat tgatgctctc catcttcctg ggcttcgagc tcatttccaa
     1321 agtgcctgcg acgctgcaca cgcctctgat gtcgggcgcg aacgcgatct cgggcatcac
     1381 cctcgtcggc gcgatcacat ccgcgggcgc gggggccgac cagatcgcga cgctgctggg
     1441 cgctgtcgcc gtcggttttg ccaccatcaa cgtggtgggc ggttacctcg tcaccaatcg
     1501 catgttgtcc atgttcaaga ggaaagacac cggtggtcag tcggggggtc ggtcatgagc
     1561 cttgaactga tcaacctctg gtacatcgtc gccgccgcgc ttttcatatt cggcctcaag
     1621 cagctcagct cgccggacac ggccgtgcgt ggcaacctgc tgtcatccgt gggcatgctc
     1681 attgccgtgg tggtgaccct gttctcccgg gatatcctcg agttccagtg gattgcggcg
     1741 gctgctgccg ttggcgcgat catcggggcc gttgccgcgc agagagtggc catgacgagc
     1801 atgccggaga tggtcgccct gtttaacggc tcgggaggca ttgccagcct gctggttggc
     1861 tgggcggccc tctacctcgg cgatggcgcc gccgccgagg ccgcagtggc cgacaccgcc
     1921 gtcgtcacgg tgggtctttc tacctttacc atcatcacca tcctgctctc cgtgctgatt
     1981 ggcggcttga ccttctcggg cagcctcatc gcctggggca agctgtcaga ggtgatgacg
     2041 agccgggcca tcgtcttcgg ggctcagcgc ctggtcaacg ccgctctgct gatcggtctc
     2101 gttgcctgtg gcgtgatgtt cgccatggag ccgtcgctga cctcacccta tctctacgcg
     2161 gtaatcgcgc tgtcgctcat cttcggcgta atggcggtca ttcccatcgg tggcgccgat
     2221 atgccggtgg tgatctcgct gctcaacagc tactccggtc ttgccgcatg cgcggcggga
     2281 tttgcgatca acaacaacat cctcatcgtc gcgggttcac tggtgggcgc tgcgggcatc
     2341 atcctgacca acatcatgtg caaggccatg aaccgttccc ttgccaacgt gcttttctcc
     2401 ggcttcggtg ccgtcaagac cgccaccaag gtcgagggcg aggtcaagcc gatcacgccg
     2461 gacgacgcgt tcctcattct ggaagcagcc tcatcggtta cgttcgtccc gggttacgga
     2521 atggccgttg cccaggcgca gcacgtggtt cgggagcttg gtgagcttct cgaggaaaac
     2581 ggtgccgagg tgacctacgc catccatccg gtggccgggc ggatgccggg gcacatgaac
     2641 gtcctgctgg ccgaggcgaa cgtgccttac gatcagctgg tcgagatgga tgacatcaac
     2701 ccccgcatcg acaacatcga cgttgcggtc gtgatcggcg ccaacgacgt ggtcaatccg
     2761 gctgcccggg aggacgagaa cagcccgatc tacggcatgc ccatcatcaa cgtcgaccag
     2821 gcgcgaaccg tgttcgtcct caagcgatcg atggcttccg ggttctcggg cgtggacaac
     2881 ccgctgttct tcggcgagaa cacgcggatg ctgttcggcg acgccaaggc gtcgatctcc
     2941 tcggtgatcg ccgagttcaa agggtagccg acaggccttt cagggcgtag acacgcccga
     3001 caccgccgag atgaacccat cgaggttggg catcgtgata cccggcagcg tggtccggat
     3061 cgcgttgcac tggagggttc tctgccaacg ctagggcttt ccggagatct cgacctccac
     3121 cagataaggc gcgtttgcgg cgaacgcgcg ctcgatggcg gagccgatct tctccggccg
     3181 tgtgaccagc tctccctcca ttcccattcc ctcggccatg gcgggaaaac ccagtgccgg
     3241 tctggtgaga tccatgtgcg ggtacggctt gttggaccct gcatcgaagc gttggcggta
     3301 aatgtcgaga ttgtgtttga gaatccgata ttcacggttc gacaggatca cgaacacgat
     3361 gggcagcccg tgatgcaccg cggaccacag ggcctgaatg ctgtacatgg cagagccgtc
     3421 tccggagatc gccaggacgc gccgattggg ctgagccacc tgaacgccca gggcgccggc
     3481 caggccctgg ccgataccgc cgcccgagcc cgcgaagaag tcgccggggg cggcgtaatc
     3541 gaatcgcgat gccacttcca acgatgcggt gatggattcg tccacgacga tggcgccttt
     3601 gggcagcgcg cggctgattt ccagcaaggc cctggctggc gtcatcggcg aggtatcgcc
     3661 ctggcgctcg atccgatccg cggcggcatc gccggcggcc cgatgggatt cggccagcgc
     3721 gtcgtttcgc gcctttgccg ccgccccgaa gtcgtcgggc gcgccctcgg cgatttcctc
     3781 caccagatgc tcgatcgttg tccgcaggtt gccgaccaga ccgagatcgg gggccaggtt
     3841 cagtgccaga cgggcatgac tggactccac gtgaacgacc cgggcctcct cgggcaacgc
     3901 gttttccgca tcgaaccaga tctcctcgaa gaacgggccg cccatcagca gaatcagatc
     3961 atggccggcg agcgcgcggc gaatggcgcc ggcttcgaac ggcaggcgtc ccttgctgtt
     4021 ggggtgcacg ttcgggaagg aaatctgagc ccgcaaaccc tgctgccata cggcggcgcc
     4081 ggtctgcacg gcgagtgtga tcagcgcccg gtgggcaccc tcggcggcga tgtcgtcgcc
     4141 gacgacgatg gctgggtttg cgctcccggc cagccagcgc gccagtcggc ggatgccctc
     4201 ggcgtccgcg agcgtcccgt agcggagatc ccctgcggta gtggcgccaa cgctggtttc
     4261 ctgctccatg acgtccaccg gcagggacat gaaaaccggg ccccgcggcg gctcgttcgc
     4321 gatcttgaac gcccgccgaa gcatggggcc gatctcgtcg gcgctgcgtg gctcggcgct
     4381 ccacttgcag accggctcgg ccatggcgac gaggtcgtga gcgagcagag gttcccgcct
     4441 gcgcatgcgg gtgtcctgct gccccgcggt gatgatgagc ggcgagttgg ctttcagcgc
     4501 gccgtagatc atcccgatgc cgttgccgag cccgggagcg acgtgcaggt tcacgatgcc
     4561 cgtcttgccg ctcgcctggg cgtagaagct cgcggcgc
//
LOCUS       DBNHQR010000523         2022 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_874513, whole genome shotgun sequence.
ACCESSION   DBNHQR010000523 DBNHQR010000000
VERSION     DBNHQR010000523.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_874513"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="AC16VR_11935"
     CDS             complement(<1..653)
                     /locus_tag="AC16VR_11935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HIR6011910.1"
                     /translation="MPEFQGFPPRTLKFLKALERNNDRDWFRENKALYERDVLDPSLD
                     FIATMGPKLAAISDHFVAIPKRTGGSLMRVYRDTRFSKDKTPYKTNIGIQFRHELGKD
                     VHAPGYYLHIDTAGCFVAAGMWRPDAPSLARVRELIAERPGDWQKARDARPFKAAFEL
                     GGESLKRPPKGFAPDAPHLQDLRRKDFIASANVATGELGQPQLVDVVAELYARATPF"
     gene            complement(716..>2022)
                     /locus_tag="AC16VR_11940"
     CDS             complement(716..>2022)
                     /locus_tag="AC16VR_11940"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6011911.1"
                     /translation="PTNGVSSFPMAGVWQELDRIVRPQGQVFYGWWIVLASSGVQWLA
                     AVLWMQSYGAYMVLLQQDFGWSKAMVAGAFALTRIESGILGPLQGWLVDRFGPRAILT
                     IGTVLFGVGFILFGQVESLLTFYLTFALIALGASLGGFATLMVAIVNWFSRHRSKAIA
                     VSQLGFSLGGLSVPLIIWALEAFGWRATAVVSGLLVLVVGLPLVQVVRHRPEAQGELA
                     DGHAASGGGPEGRFSLGGDFTARQAMRTRSFWLISFGHALALLTVSAMMVHLVPHLTE
                     GMGYSLSSAGMVVAVLTAFQLLGQLSGGYLGDLFNKRLICGVCMFAHAGGLLLVTYAD
                     SIVGVLAFTLLHGLAWGTRGPLMVSLRADYFGPTSFGTIMGFSSLIVMLGMSGGPIIA
                     GIMADVSGDYHSGFTLLASLSFMGSLCFFAATPPSPPARASA"
BASE COUNT          427 a          658 c          655 g          282 t
ORIGIN      
        1 atgaagggcg tcgccctcgc gtaaagctcc gccacgacat cgacaagctg cggctggcca
       61 agctcgccgg ttgcaacgtt cgcgctggca atgaagtcct tgcgcctgag atcctgcaga
      121 tggggcgcgt ccggcgcgaa gcctttcggc ggccgcttca gcgactcgcc ccctagctcg
      181 aatgccgcct tgaaggggcg ggcgtcacgc gccttctgcc agtccccggg ccgctcggcg
      241 atgagctccc gcacgcgggc aagcgacggc gcatcaggac gccacatgcc cgcggcaacg
      301 aagcatccgg cggtgtcgat gtgcagatag taaccgggcg cgtgaacgtc tttgccgagc
      361 tcgtgacgga actggatccc gatgttggtc ttatacggcg tcttgtcttt agagaagcgg
      421 gtatcccgat agacgcgcat gagcgacccg cccgtccgct tggggatcgc cacgaaatgg
      481 tcggatatgg cggccagctt ggggcccatg gtggcgatga aatccagcga cgggtcaaga
      541 acgtccctct cgtagagcgc cttgttctcc cggaaccagt cccggtcgtt gttgcgttcc
      601 agggccttca gaaacttcaa cgtccggggc ggaaaaccct gaaactcagg cattggcccg
      661 ctcctcacca atcatcgaga aaggatggga gcatacccag cttccggccc gagcgtcatg
      721 ccgatgcgcg tgcaggcggg ctgggaggcg tggcggcaaa gaagcacagc gaacccatga
      781 acgacaggct ggccagcagg gtgaacccgc tgtgatagtc gccggaaacg tcggccatga
      841 tgcccgcgat gatcggcccg cccgacattc ccagcatcac gatcagagac gagaagccca
      901 tgatggtgcc gaaagaggtg ggaccgaaat aatcggcccg cagcgagacc atgaggggcc
      961 ctcgggtgcc ccaggcaagg ccgtggagca gcgtgaacgc cagcacgccc actatggagt
     1021 ccgcgtaggt gacgagcagg agcccgccgg catgagcgaa catgcagacc ccacagatca
     1081 ggcgcttgtt gaacagatcg ccgagatagc cgccggagag ctgaccgaga agctgaaagg
     1141 cggtgagcac ggctaccacc atgcccgcgg aggacagcga gtagcccatg ccctcggtca
     1201 gatgaggcac caggtgaacc atcatggccg agaccgtgag cagggccagc gcgtgaccga
     1261 aggagatcag ccagaacgag cgcgtccgca tggcttgtct ggccgtgaag tcgccgccca
     1321 gggagaagcg tccctccgga ccgccgccgg aggccgcgtg cccgtctgcc agctccccct
     1381 gagcctccgg tcgatggcgc accacctgta ccagaggaag gccgacgacg agcaccagca
     1441 ggccggacac cacggcggtg gcgcgccagc cgaacgcctc cagcgcccag atgatcagcg
     1501 gcaccgagag cccgccgagg gagaagccga gctgggatac cgcgatggcc ttcgaccggt
     1561 ggcgactgaa ccagttgacg atggccacca tcaacgtggc gaaacctccc aggctcgcgc
     1621 cgagcgcgat gagcgcgaac gtcagataga aggtcagcag ggactccacc tggccgaaca
     1681 ggatgaaccc gaccccgaag agcaccgtgc cgatggtgag gatcgcgcgg ggaccgaatc
     1741 ggtccaccag ccagccctgg agcggtccga ggatgccgct ctcgatccgg gtcagggcaa
     1801 acgcccccgc aaccatggct ttgctccacc cgaaatcctg ctgaaggagc accatgtaag
     1861 ccccgtagga ctgcatccaa agcacggcgg ccagccattg gacaccgctg ctggccagca
     1921 cgatccacca gccatagaaa acctgtccct gaggccgcac gatgcggtca agttcctgcc
     1981 agacgcctgc catgggaaaa gatgatacgc cattggtcgg cc
//
LOCUS       DBNHQR010000524         2485 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_811977, whole genome shotgun sequence.
ACCESSION   DBNHQR010000524 DBNHQR010000000
VERSION     DBNHQR010000524.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_811977"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2485)
                     /gene="msbA"
                     /locus_tag="AC16VR_11945"
     CDS             complement(<1..>2485)
                     /gene="msbA"
                     /locus_tag="AC16VR_11945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005319 - lipid carrier activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HIR6011912.1"
                     /translation="VALVRGSGEVIGEMLLSQISFGVVHNIRTALFDRLLLMPSAYFD
                     ASSQGHLVSRLTYNVAQLRDAGTDALKTIIQDGGKVIVYLGYLFYLSWKLTLIFVAAA
                     PLVGLVAVYASRRFRRISRRIQNSMGDVTHVASEAVSGYRVVRIFGGEAYEQARFHKS
                     SRSNRRQNLKMVATKVSSTQAIQVLVAVALALLIGLLFRPEIGGTLSTGQVVTFLGLS
                     GLLARPIRKLSEVNARLQRGLAAAEDVFGQMDADTESDLGSMTTERVAGRLEFRDVSF
                     TYERGSAPVLSHVSFTVEPGKTVALVGRSGSGKTTIASLIPRFYEPTGGEILLDGVPL
                     PRYRLEALRSQIALVTQQVTLFNDTLERNIAYGALAGADPDEVDRAVRRAHADRFVSE
                     LPDGLQTVVGDDGVLLSGGQRQRVAIARALLKDAPVLILDEATSALDTESERHIRAAL
                     DEVMRGRTTLVIAHRLSTIENADEILVVDEGRIVEAGTHQALLNRGGHYAALYQAQFD
                     DEPDSAAVAEAPTGSTAPGLTGKLTVGQTLRPLAQAWYTGSRWTVLLAPFAWAFERYA
                     VRRRLRFLTGKRRSWRSPVPVIVVGNITAGGTGKTPMVIWLVEQLRALGYSPGVVSRG
                     YGGKRGRKPLLVTPDSDPAVVGDEAPMIASRARCPVVVAADRARAVESLLQAANSDVV
                     VSDDGLQHYALARDLEIALIDGHRGVGNGRCLPAGPLREPVSRLESVDWVISNGLGSG
                     IVARETVMTLIPTAFVRVTPGPGEARQRPEDFVAAHPNVNAVAGVGNPARFALALREL
                     GLAPVLHAQPDHHPYDGSELVFDNDWPVVC"
BASE COUNT          449 a          841 c          790 g          405 t
ORIGIN      
        1 gcagaccacc ggccagtcgt tgtcgaagac tagctcgctg ccgtcgtagg gatgatgatc
       61 gggctgggca tgaagaaccg gggcaagccc cagctcgcgc aacgccagcg caaatctggc
      121 gggattgccc acaccggcga cggcgttgac gttcggatga gcggccacga aatcctccgg
      181 acgctggcgt gcctctcccg gtcccggcgt gactcgaacg aaagcggtgg ggatcagcgt
      241 catgacggtc tctcgggcca cgatcccgga tcccaggcca ttgctgatca cccagtcgac
      301 gctctcgagc cggctgacgg gctcgcgtag cggcccggcg ggaaggcagc ggccattgcc
      361 gacgcctcga tgaccatcga tcagcgcgat ttcgagatcc ctcgccaggg cgtagtgctg
      421 cagcccgtcg tccgagacga ccacgtcgct gtttgccgcc tgcagaagac tctctaccgc
      481 cctggcacga tcggctgcca ccaccaccgg gcagcgggcc cggctcgcga tcatgggcgc
      541 ttcgtcaccg acgacggcgg ggtccgaatc cggggtcacc agcaggggct tgcgtccccg
      601 tttaccgccg taacctctgg agacgactcc cggcgagtac ccgagcgccc tcaactgctc
      661 gaccaaccag ataaccatgg gcgtcttgcc cgtaccaccc gcggtgatgt ttcccaccac
      721 gatcacggga accgggctgc gccaggagcg gcgcttgccg gtaagaaagc gcaacctgcg
      781 acgcaccgcg tagcgttcga acgcccatgc gaaaggcgcg agcagcaccg tccagcggct
      841 gcccgtgtac caggcctggg ccagggggcg cagggtctgg cccaccgtca gcttgccggt
      901 caggcccgga gccgtggacc cggtgggtgc ctcggccacc gcagcggaat ccggctcgtc
      961 gtcgaactgg gcctgataga gcgcggcgta atgtccaccc cggttcagca gcgcctggtg
     1021 agtacccgcc tctacgatcc tgccttcatc aacgacgaga atctcgtcgg cgttctcgat
     1081 ggtggacaga cgatgggcaa tgacgagcgt ggtgcgtccg cgcatcacct catccagcgc
     1141 cgcccggata tgccgttcgg actccgtgtc cagagccgac gttgcctcgt ccagaatgag
     1201 caccggcgcg tccttgagca gggccctggc gatggcgacc cgctggcgct gacctcccga
     1261 aagcagaacg ccgtcgtcgc ccaccaccgt ctgcagtccg tccggaagct cggagacgaa
     1321 ccgatccgca tgagcccgcc tcaccgcgcg atcgacctcg tccggatcag ccccggccag
     1381 ggctccgtag gctatgttgc gctcgagggt atcgttgaag agcgtcacct gctgggtcac
     1441 aagggctatc tggctgcgca gagcttccaa ccggtagcgc ggaagcggca ccccgtccag
     1501 gagaatctcg ccccctgtcg gctcgtaaaa ccggggtatc aggctcgcga tggtggtttt
     1561 gccgctgccg gagcgaccca ccagagccac ggtttttccc ggctcgacgg taaagctgac
     1621 gtggctgagc accggtgcgc tgccgcgttc ataggtaaag ctgacgtcgc gaaattccag
     1681 tcgacccgcg acgcgttcgg tcgtcatgct acccagatcg ctctcggtgt cggcatccat
     1741 ctgcccgaaa acgtcctccg ctgcggcaag accacgctgc agtcgggcgt tcacctccga
     1801 gagtttgcgg atcggccttg cgagcagccc gctcaagccc agaaaggtga ccacctgacc
     1861 cgtgctgagg gttccgccga tctccgggcg gaacagcagg ccgatgagca gcgccagggc
     1921 cacggcgaca agcacctgga ttgcctgagt gctggaaacc ttggtcgcca ccattttcag
     1981 attctggcga cgattggacc ggctcgattt gtggaaccgg gcctgctcgt aggcttcccc
     2041 gccgaagatc cgcacgacgc ggtacccgct aacggcttcg gaagccacgt gagtgacgtc
     2101 gcccatggag ttctgaatgc gccggcttat ccgcctgaaa cgccggctgg cgtagacggc
     2161 caccaggccg accagcggag ccgcggcgac gaagatcagc gtcagcttcc agctcaggta
     2221 gaacaggtaa cccaggtaga cgatcacctt cccgccatcc tggatgatcg ttttgagcgc
     2281 gtcggtaccc gcatcgcgca gctgcgccac gttgtaagtg aggcgcgaga ccaggtgtcc
     2341 ctgagagctg gcatcgaaat acgcgctggg catgagcaga agccggtcga acagcgccgt
     2401 gcggatgttg tgaacgacgc cgaacgaaat ctggctgagc agcatctcgc cgatcacctc
     2461 gccgctgccg cgaacgaggg cgacc
//
LOCUS       DBNHQR010000525         6897 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1319875, whole genome shotgun sequence.
ACCESSION   DBNHQR010000525 DBNHQR010000000
VERSION     DBNHQR010000525.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6897)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6897)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6897
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1319875"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /gene="argB"
                     /locus_tag="AC16VR_11950"
     CDS             <1..803
                     /gene="argB"
                     /locus_tag="AC16VR_11950"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014821799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HIR6011913.1"
                     /translation="EEDLENAFARDIVLLKLVGMNPVVVHGGGPQIGELLDRLNIPTR
                     FVKGMRVTDAETMDVVEMVLGGLVNQDIVSLINKNGGRAVGITGKDGDLIRARRLALS
                     ADAAELAPSEIIDIGHVGEVESVNPAVLDALVRSNFIPVIAPIGVGPDGESYNINADL
                     VAGRLAEELRAEKLILLTNTAGILDANGQTLTSLSVAEINELIAAGTIGSGMLPKAQC
                     AVSAAERGVGSTQIIDGTIPHALLLEIFTDAGIGTQITTAEASGSDAS"
     gene            793..1377
                     /gene="slmA"
                     /locus_tag="AC16VR_11955"
     CDS             793..1377
                     /gene="slmA"
                     /locus_tag="AC16VR_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HIR6011914.1"
                     /translation="MPPRSGRRDEILSAFARMLESNPGTRITTAALAGQIGVSEAALY
                     RHFPSKAKMIEGLIEFIEESLFSRINRILAEEASAASRCRAVLWLLLSFAERNPGLAR
                     LLVGDALQGETQRLRARMRQVFERLETELRSIIRDWSAARVPAPTLGATTAANLLLAS
                     AEGRISQFVRSEFRALPTSGWEEQWKALEGAVFG"
     gene            complement(1402..2007)
                     /locus_tag="AC16VR_11960"
     CDS             complement(1402..2007)
                     /locus_tag="AC16VR_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011915.1"
                     /translation="MVAILLMPSWASANRLYRYLDASGHIAISHTIPSDRVKFGYEIV
                     DAGSGRVIEVVEAQKSPEEIARLEQEAAARKACEDALERVYSVYQSELDIENAEEQML
                     ESLEQRIANAEFNLERAQSQKDGFEATAARLERSGKELDTSLIANIERSQVQVRSLET
                     EIVQRRHEQAKAKERFARDRALFRQEICVEQTAISAVTETN"
     gene            complement(2103..2771)
                     /gene="pyrE"
                     /locus_tag="AC16VR_11965"
     CDS             complement(2103..2771)
                     /gene="pyrE"
                     /locus_tag="AC16VR_11965"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013981594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HIR6011916.1"
                     /translation="MSASRNDRSLVAFLIEQGVLAFGDFTLKSGRRSPYFFNLGALNT
                     GSALARLGAAYAERIRRLDVPFDVVFGPAYKGIPIAVSTAEALAALGGDVGWAFNRKE
                     AKSHGEGGLFVGADVSGRVMLVDDVLTAGTAVREAAALIGSAGAEIAGVVIALDRQER
                     VAPNSGETAVSALELELGAPVVSILKLQDVIEYLDLDVDRDKHRAELIREMKRYRDEH
                     CVLN"
     gene            complement(2768..3487)
                     /gene="rph"
                     /locus_tag="AC16VR_11970"
     CDS             complement(2768..3487)
                     /gene="rph"
                     /locus_tag="AC16VR_11970"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016495384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HIR6011917.1"
                     /translation="MTETQVQRPSGRAPSEMREVRFTRGFTKHAAGSVLVEFGDTKVI
                     CTASVENSVPGFLRGKGVGWVTAEYGMLPGATHVRTDREAARGKQGGRTQEIQRLVGR
                     ALRASVDMQALGERTIRLDCDVIQADGGTRTASITGSALALADAIASAGCNGALKELV
                     ASVSVGLWRGQPVLDLDYAEDSTAETDMNVVMLESGGFIEIQGTAEAGAFSDSELMSM
                     LALARSGTAELLRRQRQALSS"
     gene            3642..4496
                     /locus_tag="AC16VR_11975"
     CDS             3642..4496
                     /locus_tag="AC16VR_11975"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HIR6011918.1"
                     /translation="MTAFARVQGSAKGLSLIWEMRSVNHRFLETQFRLPEQMRPLEHG
                     LRETLRRHVKRGKFDCSLKIDRSTGGDGIELNRPLLLQLLAAVEQIRRDAPEIGPGNA
                     MDVLRWPGIIGDASTLEDEPLVQAATDLFEEALVQLIDNRQREGAQLASVISERLDEI
                     DSIAREVKALTATMSHEVRSRLTQRLNDLKVQADEGRLDQEVALLAQRADVAEELDRL
                     VIHVEEARSNLRGPGPHGRRLDFLTQELNREANTLGAKSVLAQTSQKAVDLKVAIEQI
                     REQVQNVE"
     gene            4493..5125
                     /gene="gmk"
                     /locus_tag="AC16VR_11980"
     CDS             4493..5125
                     /gene="gmk"
                     /locus_tag="AC16VR_11980"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - GMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HIR6011919.1"
                     /translation="MSQASRQGLLFIVSAASGTGKTSLVRALIAQDGNLAVSISHTTR
                     PRRPGDQEGVDYHFVEPKLFREMAQRGDFLEDADVFGNRYGTSKRTVDEQLRTGVDVI
                     LEIDWQGARQVRAAYPDATSIFILPPSRAALVQRLEGRGLDAANVIEARSDKAREEIS
                     HYGEYDYLVVNDDFATALSELTQIIAASRLGLQPQRERLGALLKDLLRQE"
     gene            5225..5497
                     /gene="rpoZ"
                     /locus_tag="AC16VR_11985"
     CDS             5225..5497
                     /gene="rpoZ"
                     /locus_tag="AC16VR_11985"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003195508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HIR6011920.1"
                     /translation="MARITVEDCLENVENRFELVMLATRRARQMRRFGAEPLVPEEND
                     KPTVIALREIAEGLISNEMLDAQEASTEDEELEVSFGIGDGETGEI"
     gene            5475..>6897
                     /locus_tag="AC16VR_11990"
     CDS             5475..>6897
                     /locus_tag="AC16VR_11990"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013981587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIR6011921.1"
                     /translation="MAKPARSDHQGSKHPGSRVVRKKTRAGEKPKLPQPESYFVGLLA
                     DESRRDDIAAPVSIESLTTLLSTYLEPDQVRRVERAHAYAINAHEGQFRRTGHPYISH
                     PLAVANILATMRMDHETIMAALLHDVIEDTDADRNALAQRFGKSVAEIVDGVSKLSKI
                     FRSRAEAQAENFQKMALAMAKDIRVIMVKLADRLHNMRTIGVMSREARQRIARETLDF
                     YAPIANRLGIHTMKVEFEELGFRALYPLRADRIGRAVRAARGNRKELVEELRKSLQDA
                     LDQESVTAEVIGREKHLYSIYRKMKAQHKPFAEIMDVFGFRVIVDEVEDCYRALGVVH
                     NTYKPVAGRFKDYIAIPKVNGYQSLHTTLFGMHGVPIEVQIRTRDMAAVADNGIAGHW
                     LYKSEGEDFQSSQQRARRWVKDLLDLQQRAGNPMEFVESLKIDLFPDEVYVFTPQGDI
                     LELPRGACAVDFAYAVHTDIGNRC"
BASE COUNT         1312 a         2267 c         2168 g         1150 t
ORIGIN      
        1 tcgaggaaga cctggaaaac gcgttcgcgc gcgacatcgt gctgctgaaa ctggtcggca
       61 tgaatccggt ggtcgtccat ggcggcgggc cgcagatcgg cgagctcctg gatcggctca
      121 acattcccac ccgcttcgtc aaaggcatgc gggtcacgga cgccgagacc atggacgtgg
      181 tggagatggt tctcggcggg ctggtgaatc aggacatcgt atccctgatc aacaagaacg
      241 gcggtcgcgc cgtggggata acgggtaaag acggcgatct gatcagggcg cgccggctgg
      301 cgctgagcgc cgacgcggcg gagctcgccc cgtcggagat catcgacatc ggccacgtcg
      361 gcgaggtcga aagcgtcaac ccggccgtcc tggacgccct cgtgcgcagc aacttcatcc
      421 cggtgatcgc gcccattggc gtggggcccg atggcgagtc ctacaacatc aatgccgacc
      481 tcgtcgcagg ccgcctggcg gaggagctgc gcgcagaaaa gttgatactg ctgaccaaca
      541 ccgccggcat cctggacgcc aatggccaga cactcacctc gctgagcgtc gcggagatca
      601 acgagctcat cgccgcgggc accatcggct cgggcatgct gcccaaggcg cagtgcgccg
      661 tgtccgctgc cgagcgcggc gtagggagca cacagatcat cgacggaacc atacctcacg
      721 ctctcctgct ggaaatcttc accgatgccg gaatcggtac gcagatcacc accgccgagg
      781 cgagcgggtc cgatgcctcc tagatcgggc cgccgcgacg aaattctcag cgcgttcgcg
      841 cgaatgctgg aaagcaaccc gggaacgcgg ataaccaccg ccgccctggc cggccagatc
      901 ggggtatccg aggccgccct ctaccggcac tttcccagca aggcaaaaat gatcgagggg
      961 ctcatcgagt tcatcgagga gagcctcttc agccgcatca atcgcattct cgcagaggag
     1021 gccagcgccg cgtcccgctg ccgggccgtg ctgtggctgc tgctttcctt cgccgagcgg
     1081 aatccgggac tggcgcgctt gctggtcggg gacgccctgc agggcgagac ccaacggctt
     1141 cgggcgcgca tgcgccaggt tttcgagcgg ctggaaaccg agcttcggtc gatcatccgg
     1201 gactggtcgg ccgcccgggt gccggcacca acgctcggtg ccacgacggc cgccaacctg
     1261 ctcctggcca gtgcggaggg tcgcatcagc cagttcgttc gaagcgaatt tcgcgcgctg
     1321 ccgacctccg gctgggagga acagtggaag gctctggaag gggccgtctt cggctagcca
     1381 gccaaggaac ggcaaacgcc ctcagttcgt ttccgtaacc gcgctgatgg ccgtctgctc
     1441 gacacagatt tcctggcgga aaagagcccg atcacgggcg aaacgttcct tggctttcgc
     1501 ctgctcatgg cggcgctgaa cgatctccgt ctccaggctc ctcacctgaa cctgggacct
     1561 ctcgatattg gcgatgaggg aggtgtccag ttccttgccg gagcgctcga gtcgggcagc
     1621 ggtggcctcg aagccgtctt tctggctttg cgcgcgctcg aggttgaact cggcgttcgc
     1681 aatgcgctgc tctagagatt ccagcatctg ctcttctgcg ttctcgatat cgagctcaga
     1741 ctggtacacc gaatagacgc gctcgagcgc gtcttcgcac gccttgcggg cggccgcttc
     1801 ctgttccagc ctggcgattt cctcagggct cttctgggcc tccacgacct cgatcacccg
     1861 cccgctgccg gcgtcgacga tctcgtagcc gaacttgacc cggtcgctgg ggatggtgtg
     1921 agagatcgct atgtgaccgc tggcatcgag gtaccggtac agacgattgg ccgaagccca
     1981 ggacggcatc aggaggatcg ccaccatgat caagaccgcc gcccgcgggc gacattggaa
     2041 attgcctcgg tatcgtgttc ccatcaatcg ctcaatcgtc gagttatgag cgactagccg
     2101 gttcagttca gaacgcaatg ctcgtcgcgg taccgcttca tttcgcggat tagttcggct
     2161 cgatgtttgt cccgatcgac gtccagatca aggtactcga tcacgtcttg gagcttgaga
     2221 atgctgacta ccggtgcgcc aagctccagc tccagggcag aaaccgccgt ttcccctgag
     2281 ttcggggcta cgcgctcctg tctgtcaagc gcaatgacaa cgccggcaat ctccgcgcct
     2341 gcagatccaa tgagcgcggc cgcttcccgc acggcggtac ccgcggtaag cacgtcgtcc
     2401 acgagcatga ccctgcccga gacgtctgcg cctacgaaga ggccgccctc cccatgggac
     2461 ttggcttcct tcctgttgaa tgcccaacca acgtcgccac caagagcagc aagcgcctcc
     2521 gcagtggaaa ccgcaatggg aatgcccttg taagcgggac cgaacaccac gtcgaacggc
     2581 acgtccagcc ggcgaatgcg ctcggcgtag gcggcgccca gccgcgccag ggctgaaccc
     2641 gtattcagag cccccaggtt gaagaaatag gggcttctgc ggccggattt cagcgtgaaa
     2701 tccccgaacg cgagaacgcc ctgttcgatg agaaacgcca ccagggagcg atcgttccgg
     2761 gaggcgctca agagctcagc gcctgtcgtt ggcggcgcag cagctccgcc gtgcccgacc
     2821 gagccagggc cagcatgctc atcagctcgc tgtcgctgaa cgcccccgcc tcggccgttc
     2881 cctgaatctc gatgaaccca ccggattcca gcatcaccac attcatgtcg gtctccgccg
     2941 tggagtcctc ggcgtaatcc agatccagca cgggttgacc gcgccacaga ccaaccgaca
     3001 ccgaggcaac cagctccttg agcgccccgt tgcaaccggc cgaggcgatg gcgtcggcca
     3061 gcgccagggc ggagcccgtt atggatgccg tccgcgtgcc gccgtctgcc tggatcacgt
     3121 cgcaatccag ccggatggtg cgctcgccga gcgcctgcat gtccacggac gcccgcaagg
     3181 cgcgcccgac gagccgctgt atttcctgcg tgcgaccgcc ctgcttgcca cgcgctgcct
     3241 ctcgatcggt tcgcacatgg gtggcgccgg ggagcatgcc gtactcggca gtcacccagc
     3301 ccacgccctt gcctcgcagg aagccgggca ccgagttctc cacgctggcc gtacagatca
     3361 ccttggtatc accgaactcc acgaggacgg agcccgcggc atgcttggtg aatccgcggg
     3421 tgaagcggac ttcccgcatc tctgagggcg cccggccact gggacgctga acctgtgttt
     3481 cggtcatcaa aatcctcggg acggcagaag tgggcgggca ttataaaggc aacggccggc
     3541 agcaacgcga tcgcgcggcg cggttggatg gcagggcccc cgcccgggta gaatgcgcgc
     3601 tcgccatttt acgtgccgct gacctaccga tgatccacag catgaccgca ttcgcccgcg
     3661 tccagggcag cgccaagggg ctctcgctga tctgggagat gcgatcagtg aatcaccgtt
     3721 ttctggaaac tcagttccgg cttcccgagc agatgcgccc gttggagcac ggcctgcgtg
     3781 aaaccctgcg gcgtcacgtc aagcgcggca agttcgactg ctcgctgaag atcgaccgaa
     3841 gcaccggcgg cgacggtatt gagctcaacc gcccgctgct cctccaactg ctcgccgcgg
     3901 tcgagcagat ccgccgggac gctccggaga tagggcccgg caacgccatg gacgtcctgc
     3961 gctggccggg catcattggc gatgcgtcaa ctctggagga cgaacccctc gtccaggccg
     4021 ctactgatct gttcgaggaa gcgctcgtcc agctcatcga caatcggcaa cgggagggag
     4081 cgcagctcgc ctccgtcatc tccgagcggc tggacgagat cgacagcatc gctcgcgagg
     4141 tcaaagcgct gaccgcgacg atgagtcacg aggtgcgctc caggctgacc caacgactca
     4201 acgatctgaa ggttcaggcc gacgaaggcc ggttggacca ggaagtcgcc ctgctggccc
     4261 agcgggccga cgtcgccgag gagctcgacc ggctggtcat tcacgtggag gaggcccgca
     4321 gcaacctgcg cggcccgggg ccccacgggc gccggctcga ctttctcacc caggaactta
     4381 accgggaagc caatacgctg ggcgcgaagt cggtgctggc ccagacgtcg cagaaggcgg
     4441 tggacctgaa ggtcgccatc gagcagatcc gcgagcaggt tcagaacgtc gaatgagcca
     4501 agcgtctcgc cagggcctgc tgttcatcgt ttccgcagcc tctggaacag gcaagacgag
     4561 cctcgttcgt gcgctcatcg ctcaggacgg caacctggcc gtttcgatct cccacaccac
     4621 gcgtccgcgc aggccgggcg atcaggaagg cgtggattac cacttcgtcg aaccgaaatt
     4681 gttccgggag atggcgcaac gaggggactt cctggaggac gccgacgtct tcggaaaccg
     4741 ctacggaacg tcgaaaagga ccgtcgacga gcagctgcgc acaggcgtcg acgtcattct
     4801 cgagatcgac tggcagggtg cccggcaggt tcgcgccgct tacccggacg cgaccagcat
     4861 tttcatcctg ccgcccagcc gcgccgccct ggtgcagcgg ctggaggggc ggggcctgga
     4921 tgccgccaac gtcatcgagg cgcgcagcga caaagcccgg gaggagattt cgcactacgg
     4981 ggagtacgac tacctggtgg tgaacgacga cttcgccaca gccctgagcg agctgacgca
     5041 gatcatcgcg gcgagccggc tcgggctgca gccccagcgg gagcggctgg gcgcgctgct
     5101 caaagacctc ctccggcagg agtgacggca ctttacaatc ttccggaagg gcggctacac
     5161 tcgcgcgctc gctgcacacc ggctctctcg gagccacttt agaagatcgc atcagaggtt
     5221 cgccatggcc cgcatcaccg tcgaggattg cctggaaaac gtagaaaacc gcttcgagct
     5281 cgtgatgctc gcaacgcggc gggcacgcca gatgcgccgc ttcggcgcgg agcccctggt
     5341 tccggaagaa aatgacaaac ccaccgtgat cgctctgcgc gaaattgccg aagggctcat
     5401 atcaaatgaa atgcttgacg cacaagaagc ctccacggag gatgaggaac ttgaggtttc
     5461 tttcggtata ggagatggcg aaaccggcga gatctgacca ccagggctcc aagcaccccg
     5521 ggtctcgcgt tgtccgcaag aaaacacgcg ctggcgaaaa accgaagctc ccccaacccg
     5581 aatcctactt cgtcggcctg ctcgccgacg agtcgaggcg cgacgacatc gccgcgccgg
     5641 tatcgatcga atccctgacg accctgctgt ccacttatct ggagccagat caggttcgcc
     5701 gcgtcgagcg cgcccacgcc tacgccatca acgcccacga agggcagttc cgccgcacgg
     5761 ggcaccctta catctcgcac cccctggcgg tagcgaacat cctcgccacg atgcgcatgg
     5821 atcacgagac gatcatggcc gctctgctcc acgacgtcat cgaggacacc gacgcggacc
     5881 gcaacgccct ggcccagcgt ttcgggaagt cggtggcgga gatcgtcgac ggggtgtcaa
     5941 agctgtcaaa gatattccgc tcccgagccg aggcccaggc ggaaaacttt cagaagatgg
     6001 ctctggccat ggccaaggac atccgggtga tcatggtgaa gctggccgac aggctgcaca
     6061 acatgcgcac catcggcgtc atgtcccggg aggcgcgtca gcgcatcgcg cgggagactc
     6121 tggatttcta cgcgcccatc gccaaccggc tcgggataca caccatgaag gtggagttcg
     6181 aggagcttgg tttccgggcc ctgtacccgc tgcgcgccga ccgaatcggc cgggctgttc
     6241 gtgccgcccg ggggaaccgg aaagagctgg tcgaagagct gcgtaaatcc ctccaggacg
     6301 cgctggacca ggaatcggtc acagcggagg taatcggtcg cgagaagcac ctctattcca
     6361 tctaccggaa gatgaaggcg cagcacaagc cgttcgccga gatcatggac gtgttcgggt
     6421 tccgcgtgat cgtggacgag gtagaggact gctatcgggc cctcggggtg gtgcacaaca
     6481 cctacaagcc ggttgccggc cgcttcaagg actacatcgc gatcccgaag gtgaacggtt
     6541 accagtcgct gcacaccacg ctcttcggca tgcacggcgt tcccatcgag gtgcagattc
     6601 gcacgcggga catggcggcc gtcgccgaca acggcatcgc cggccactgg ctgtacaaat
     6661 ccgaagggga ggatttccag agcagccagc agcgggcgcg gcgctgggtc aaggatctgc
     6721 tcgatctgca gcagcgtgcg ggcaacccga tggaattcgt ggaaagcctc aagatcgacc
     6781 tgttccctga cgaggtgtac gtgttcacgc cccagggcga cattctcgag ctgccccgcg
     6841 gcgcctgcgc cgtggatttc gcctacgcgg ttcacacgga catcggcaac cgctgcg
//
LOCUS       DBNHQR010000526         3935 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1255795, whole genome shotgun sequence.
ACCESSION   DBNHQR010000526 DBNHQR010000000
VERSION     DBNHQR010000526.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1255795"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="AC16VR_11995"
     CDS             <1..284
                     /locus_tag="AC16VR_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6011922.1"
                     /translation="EVHHPSIKSELVKEAMEIFFDVRKVPGLKKKPSTSELIDWLKLL
                     MADDIPDEILTNRDTSKAIPPLYGALVKNEQDVHLLERLAFMNRRDSRG"
     gene            326..1498
                     /locus_tag="AC16VR_12000"
     CDS             326..1498
                     /locus_tag="AC16VR_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012722828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR6011923.1"
                     /translation="MLINFFLTLRKYKVPVSIRELMDLLEALSLRLVYANIDDFYLLS
                     RTCMVKDEKHYDKFDRAFAAYFKGLEDLHGMLESLIPDEWLRREFEKSLTAEELEKIQ
                     SLGGLEKLIEAFQEARAEEERRQAEQARQGEEGEEGENPDRQGQKGPGGLGKGKKKKA
                     KKVWDQRQYKNLDDSVELGTRNIKMALRRLRKFARSGKEEELDIDTTIRSTAHNGGML
                     DIRMRPERKNTVKVLLFLDIGGSMDSHVKVCEELFSATRTEFKHMESFYFHNFIYESV
                     WKDNRRRMNERLSTWEILNKYGQDYKVVFVGDATMAPYEITHAGGSVEHWNEEPGAEW
                     MNRFTEIYDRLVWINPTPQETWEYSSSVGMTQQLVNGQMYPLTIRGLEEGMSFLSK"
     gene            1563..>3935
                     /gene="hrpA"
                     /locus_tag="AC16VR_12005"
     CDS             1563..>3935
                     /gene="hrpA"
                     /locus_tag="AC16VR_12005"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HIR6011924.1"
                     /translation="MRRDVHRLKSLRRSNPSAWQALREQSEARLLARRGQRLEIAYPP
                     ELPISQHADEITRLLADHQVIVVAGETGSGKTTQLPKMCLEAGFGYRGMIGHTQPRRL
                     AARAVAARGAEDLGARVGEAVGYAVRFSDQVGEHTLVKLVTDGLLLNEIRSDRFLDAY
                     EVIIVDEAHERSLNVDFLLGYLRRLVDRRHDLRVIITSATIDVASFSEHFGGAPVVEV
                     GGRGFPVETRYADEEALADRSAEELIVDRIRSIEKTPVAGSARDILVFQSGEREIFDT
                     AHALRQALDERYEILPLYARLSARDQQRIFKPSGYRRVVIATNVAETSLTVPNIGFVI
                     DPGFARVSRYSFRSKLQRLPVEPISQASADQRLGRCGRVAPGVCYRLYTENDYLSRPP
                     YTDPEIKRTNLAQVVLQMRSLGLGKVAGFPFLDPPDPRAVRDAERLLHELGALEGGGL
                     SKTGRMMARLPVDPRLARMIIAGHEHRSLSEVLVIVSALAAQDPRERPLERQGSADRA
                     HSEFSDPRSDFLAFLNIWQWYLEARQRLSRARLSRELGSRFISATRMREWHALHRQLL
                     LACRELALKPNDQPADYSAVHRALLQGSLSLVGLHEEKGSYLGPRNLRFRIFPGSALA
                     RRGPKWLMAGEITETRRVYARCVAGVEARWIEEAAGDLVKRRYSEPRWSVRRGEVIAL
                     ETVTLYGLPLADGRRVSYRKVDPDACRELFITEALVRGGLEGPFEFLLHNRGLIREIV
                     DEESKGRRRDLLIGDAAQADLYAARLPREVCSVSTLGKFFKQAGPEARERL"
BASE COUNT          750 a         1171 c         1291 g          723 t
ORIGIN      
        1 tcgaggtgca tcatccgagc atcaagtccg agctggtcaa ggaagcgatg gagatcttct
       61 ttgatgtgcg caaggtgccg gggctgaaga aaaagccgtc cacatccgaa ctcatcgact
      121 ggctgaagct gctgatggcg gacgacattc cggacgagat tctcaccaat cgggatacga
      181 gcaaggccat accgccgctt tacggcgccc tggtgaagaa tgaacaggac gtgcacctgc
      241 tcgagagact ggcgttcatg aatcgtcggg acagccgcgg ctagaagcgg cgggtcgata
      301 gccaccgagg gagcacgacc ggacaatgct catcaacttc tttctcaccc tgcgcaaata
      361 caaggtgccg gtctccatcc gcgagttgat ggatctgctg gaggcgctca gccttcggct
      421 ggtttacgcc aacatcgacg atttctatct gctcagccgc acctgcatgg tcaaggacga
      481 gaagcactac gacaagttcg accgggcctt tgcagcctac ttcaaagggc tggaggacct
      541 gcacgggatg ctggagtccc tcataccgga cgagtggctg cgtcgcgaat tcgaaaaatc
      601 gttgacagcc gaagagctcg aaaagattca atccctcggc ggcctggaga agctcatcga
      661 ggcgtttcag gaagccaggg ccgaggagga acggcgtcag gcagagcagg cgcgtcaggg
      721 cgaggaaggc gaggagggcg agaatcccga ccggcagggc cagaagggcc cgggcggcct
      781 tggcaaaggc aaaaagaaga aagcaaagaa agtctgggat cagcggcagt acaagaatct
      841 cgacgactcg gtggagctcg gcacccgcaa catcaagatg gcgctgcgcc ggctgaggaa
      901 gtttgcccgt tccgggaagg aagaagagct ggatatcgac accacgatcc ggtcgacggc
      961 gcacaacggc ggaatgctcg acatacgcat gcggcccgag cgcaagaata cggtgaaggt
     1021 gcttcttttc ctcgacatcg gcggttccat ggactctcat gtgaaggtct gtgaggagct
     1081 tttctcggcg acccggaccg agttcaagca catggaaagc ttctacttcc acaacttcat
     1141 ctacgagtcg gtgtggaaag acaaccgccg gcggatgaac gagcgtctgt cgacgtggga
     1201 gatcctcaac aagtacggcc aagactacaa agtggtcttc gtgggagacg ccaccatggc
     1261 tccctacgag attacccatg ccggtggtag cgtggagcac tggaacgaag agcccggcgc
     1321 ggagtggatg aaccgcttca ccgaaattta cgaccggctc gtctggatca atcccactcc
     1381 tcaagaaacc tgggagtatt cctcgtcggt cggcatgacc cagcagcttg tgaacgggca
     1441 gatgtacccc ctgacgattc ggggcctcga agagggaatg agcttccttt ccaaatagcc
     1501 tggccgacca tctcacgatg gcttctcgac gcgccgatac ctcgtttgat cccgctgccg
     1561 tcatgcggcg ggacgtccat cgtctcaaat cactgagaag gagtaatcct tccgcctggc
     1621 aggcgcttcg tgagcaatcc gaggccaggc tcctcgcccg gcgcggccag cggctggaga
     1681 tcgcttaccc gcccgagctt cccatcagtc agcatgccga cgagatcacg aggctgctgg
     1741 ccgatcatca ggtcatcgtc gtggccgggg agaccgggtc gggaaagacc actcagctgc
     1801 ccaagatgtg cctcgaggcg ggcttcggct atcgcgggat gatcgggcac acgcagccaa
     1861 ggcgtctggc ggctcgagcg gtggccgccc ggggcgcgga ggacctcggc gcccgggtcg
     1921 gtgaggcggt gggctacgct gtccggttca gcgatcaggt gggcgagcac acgctggtga
     1981 agctggtgac ggacggtctg cttctcaacg agatacgttc ggacaggttt ctcgacgcct
     2041 acgaggtgat catcgtcgac gaggcccacg agcgcagcct gaacgtggac tttctgctcg
     2101 gctacctccg caggctggtc gaccgccgcc acgatctgag ggtcatcatc accagcgcca
     2161 ctatcgacgt ggcctctttc tctgagcatt tcggcggggc accggtggtg gaggtcggcg
     2221 gacgagggtt tcctgtcgag acccgttatg cagacgagga agcgctggcg gatcgaagcg
     2281 ccgaggagct gatcgtcgac cgtatccggt ccatcgagaa gacgcccgtg gcgggatcag
     2341 cgcgggacat cctcgtgttc cagtccggcg agcgggagat attcgatact gcccacgcgc
     2401 tgcgccaagc gctcgatgag cgttacgaga tactgcctct gtacgcgcgc ctgtcggccc
     2461 gggaccagca gcgcatcttc aagccctcgg gatatcgacg ggtggtgatc gccaccaatg
     2521 tggccgaaac gtcgttgacg gtgcccaaca tcggcttcgt catcgatccc ggctttgccc
     2581 gggtgagccg ctactcgttt cgctccaagc tgcaacggtt gcctgtcgag cccatatcac
     2641 aggccagcgc ggatcagcgt ctgggccgct gcggccgggt ggcgccgggc gtgtgctatc
     2701 ggctttatac cgagaatgac tatctgagcc ggcctccata taccgatccg gaaatcaagc
     2761 gcacgaacct cgcccaggtc gtgcttcaga tgcgctcgtt ggggttgggt aaggtcgcgg
     2821 gctttccgtt tctcgacccg cccgaccccc gggcagtccg tgacgcggag cgtctgctcc
     2881 acgagctggg cgcgcttgaa gggggcggtc tttccaagac cgggcgcatg atggcccggc
     2941 tcccggtgga cccgcgcctg gcccggatga tcattgcggg ccatgagcac cggtcactgt
     3001 ccgaggttct ggtgatcgtg agcgctctgg ccgcccagga tccccgtgag cggccgctgg
     3061 aacgccaggg gtccgccgac cgggcccatt cggagttctc ggatccgcgc tcggattttc
     3121 tcgcctttct gaacatctgg cagtggtacc tggaagcccg gcagcgtctt tctcgcgcca
     3181 ggctgtctcg cgagctcggc tcgcgcttca tttccgccac gcggatgcgg gagtggcatg
     3241 cgctgcatcg gcagctgctg ctggcttgcc gtgagcttgc gctcaaaccg aacgatcagc
     3301 ccgccgatta ctcggccgtg catcgcgcgc tgctccaagg atccttgagc ctggtaggtc
     3361 tgcacgaaga gaagggcagc tatctggggc cgcgtaacct gcgattccgt atcttccccg
     3421 gatcggcgct ggccaggcgt gggccgaagt ggctgatggc cggagagatc accgagacac
     3481 gccgggttta cgcgcgctgc gtggccggcg tcgaggctcg atggatcgag gaagctgccg
     3541 gcgatctggt caagcgccgc tactccgagc cgcgctggag cgtacgaaga ggcgaggtga
     3601 tcgcgcttga gaccgtcacg ctgtacggcc ttcctttggc ggacgggcgt cgcgtgagct
     3661 atcggaaggt agacccggat gcctgtcgtg agctcttcat taccgaggcg ctcgtgcgcg
     3721 gagggctcga agggcccttc gagttccttc tccacaatcg gggcctgatt cgggagatcg
     3781 tcgacgagga atccaagggc cgccggcgcg atttgctgat cggcgatgcg gctcaggcgg
     3841 atctttacgc ggccaggctc ccgcgggaag tgtgctcggt ttcgaccctt ggaaagtttt
     3901 tcaagcaggc ggggcctgag gcgagggagc gtctc
//
LOCUS       DBNHQR010000527         2762 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_115245, whole genome shotgun sequence.
ACCESSION   DBNHQR010000527 DBNHQR010000000
VERSION     DBNHQR010000527.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_115245"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="AC16VR_12010"
     CDS             complement(<1..671)
                     /locus_tag="AC16VR_12010"
                     /inference="COORDINATES: protein motif:HMM:NF013741.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HIR6011925.1"
                     /translation="MGTSGPARALRFGSATLTVTTMYTLLITFFTLAIVFSFLCSLWE
                     AVLLSITPAYAEIQKQHGSAIGRHLSTFKSNIDRPLAAILTLNTIAHTVGAIGVGGQA
                     AAIWAETSPWVTKAIIPVAMTLAILILSEIIPKTIGATFWKELAPFTVRSLLVVIAAL
                     GPLVWLSQRVTRILKREAGDSVLSRSDFLAMTKIGERAGVFEKSESEIIKNLLRFEQV
                     CAKDI"
     gene            806..1681
                     /locus_tag="AC16VR_12015"
     CDS             806..1681
                     /locus_tag="AC16VR_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007943000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HIR6011926.1"
                     /translation="MGAIEQPFEMIRYERPGEHVARIMLAREETRNAQNVQMLYEIDA
                     ALDAAMQDDDVRAVIIGADGPHFSSGHDLKGGGRIGDFGPPKMGVGGFGKPGQEGHMS
                     HEQEVFIGFCWRWRNMPKPTIAAVQGKAIAGGLMLVWPFDLIVASEDAQFSDPVVTFG
                     VNGHEYFVHAWEVGHRKAKEMLFTGESISAQEGKELGMVNRVVKREDLMSETLALAEK
                     IARQPSMGLKLSKLAVNQSLDAQGMWTAIQSAFNLHHLGHAHMRIMHGQAVEPAGAKK
                     IKTQARADKPKLTGP"
     gene            complement(1696..>2762)
                     /locus_tag="AC16VR_12020"
     CDS             complement(1696..>2762)
                     /locus_tag="AC16VR_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007349022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HIR6011927.1"
                     /translation="NGYQRNPGREQVKRIVICADGTWNRPERNLAEDQPTNVLRLARA
                     IRPSGSTGTAQQVFYDWGIGSYYDPFVGGVTGRGINKNIMDGYRYLVQNYAPGDQIFL
                     FGFSRGAYTVRSLCGLINNCGILKRPDARLIQAAFDHYKRPGSAHHPDGPRSREFRAE
                     HAHPSRRIRFVGVWDTVGALGIPFSFLGMLDREDEFYDTKIGPNIEIARHAMAIDERR
                     SDFEPTIWQQRTGTDISQVWFAGAHGNVGGGLYREGKGGRLSDIALAWMINEARGAGL
                     SVEGHLGDRLRPDPLAPVYGSRRHIYRSRPRADRPIDHGSAPVLVHESVRQRWDALPG
                     YRPRNLVRYLEINGWPTLVP"
BASE COUNT          493 a          848 c          947 g          474 t
ORIGIN      
        1 atgatgtctt tggcgcaaac ctgctcgaag cgaaggagat tcttgatgat ctccgactcc
       61 gacttctcga agacgccggc ccgctcgccg atcttggtca tggcgagaaa gtcggagcgc
      121 gagaggacgc tatctccggc ctcccgcttg aggattcggg tgacccgctg gctcagccag
      181 acgagcggtc ccagcgcggc gatcaccacg agcagcgagc gcaccgtgaa aggcgccagc
      241 tccttccaga aggtggcgcc gatggtcttc gggatgatct ccgacaggat gagaatggcg
      301 agcgtcatcg ccacgggaat gatggccttg gtgacccagg ggctggtttc cgcccagatg
      361 gcggcggcct gaccacccac gccgatggcg ccgacggtat gggcgatggt gttgagcgtg
      421 aggatggcgg ccagcggccg gtcgatgttg ctcttgaaag tgctcaggtg gcgcccgatt
      481 gccgagccgt gctgtttctg aatctcagcg tacgcgggcg taatgctcag cagcacggcc
      541 tcccacaggg agcagagaaa agagaaaacg atggccagcg tgaagaaggt gatcagcagc
      601 gtgtacatgg tcgtgactgt gagcgttgcg gacccaaagc gcaacgccct ggccggaccg
      661 gacgttccca tggtgccggg cggaagttgc tggctgatcg gccagggatt agagtcggca
      721 gtcgccgtgc ccgggggcgc ggcttccggc aaacagggtc cgagccgcgg acgcgcgcgg
      781 gcccgccttg aattgggggg atgtcatggg tgccattgag cagcctttcg agatgatccg
      841 ctacgagcgg cccggcgagc acgtggcgcg catcatgctg gcgcgggaag agacccgcaa
      901 cgcgcagaac gtccagatgc tctacgagat cgacgcggcg ctggatgcgg ccatgcagga
      961 cgacgacgtg cgggcggtga tcatcggcgc cgacggcccg catttctcgt cgggccacga
     1021 cctgaagggc gggggccgca tcggcgactt cggacccccg aaaatgggcg tcggcggttt
     1081 cggcaagccc gggcaggaag gccacatgtc tcacgagcag gaggtcttca tcggcttctg
     1141 ctggcgctgg cgcaacatgc ccaagccgac catcgccgcc gtgcagggga aggccatcgc
     1201 cggagggctc atgctggtat ggcctttcga tctcatcgtc gccagcgagg atgcgcagtt
     1261 ctccgacccc gtggtcacct ttggcgtcaa cggccatgag tatttcgtgc acgcctggga
     1321 agtcgggcac cgcaaggcca aggagatgct gtttaccggg gagtcgattt ccgcccagga
     1381 gggtaaggaa ctcggcatgg tcaaccgcgt ggtcaagcgg gaagacctga tgagcgagac
     1441 cctggccctt gcagaaaaaa tcgctcggca gcccagcatg gggctgaagc tgtccaagct
     1501 cgccgtcaac cagtccctgg atgctcaggg catgtggacg gccattcaat cggcgttcaa
     1561 cctgcatcac ctcggccacg cccacatgcg catcatgcac ggccaggccg tggagcccgc
     1621 cggcgctaag aagatcaaga ctcaggcccg ggcggacaag cccaagctca ctggcccttg
     1681 aagcgagccg ccggctcagg gaaccagcgt cggccagccg ttgatctcaa ggtagcggac
     1741 caggttgcgc ggccggtagc ctggcagcgc atcccagcgc tggcgaacgc tttcgtgaac
     1801 gagcaccgga gcgctgccgt ggtcgatggg acgatccgct cggggccggg agcggtagat
     1861 gtggcggcgt gacccgtaca cgggcgcgag gggatcgggc cgcagccggt cacccaggtg
     1921 gccctccact gaaagcccgg cgccccgggc ttcgttgatc atccatgcca gcgcgatgtc
     1981 cgaaagccgg ccacccttgc cttcccggta gagcccgccc ccgacgtttc cgtgggcgcc
     2041 tgcaaaccaa acctgtgaga tgtcggtgcc cgtgcgctgc tgccagatcg taggttcgaa
     2101 gtcggagcgg cgctcgtcga tggccatggc gtggcgggcg atctcgatgt tgggaccgat
     2161 cttggtgtcg tagaactcat cctcacgatc gagcatcccc aggaaggaga acggaatgcc
     2221 cagcgcaccg acggtgtccc agacgcctac gaagcggatc ctgcggctgg ggtgcgcatg
     2281 ctccgctcga aactcccggg agcgcgggcc gtcgggatga tgggcgctgc ctggtcgttt
     2341 gtaatgatcg aatgccgcct ggatgagcct ggcgtcaggg cgcttgagga tgccgcagtt
     2401 gttgatcagc ccgcacaggc tgcggaccgt gtaggcgccg cggctgaagc cgaacaggaa
     2461 gatctggtcg ccgggggcgt agttctgcac caggtagcgg tatccgtcca tgatgttctt
     2521 gttgattccc cgtcccgtca cgccgccgac gaagggatcg tagtaagagc cgatccccca
     2581 gtcgtaaaac acctgctggg cggtgcccgt gctgcccgat ggccggatgg ccctggccag
     2641 ccgcaggacg ttggtgggct gatcttcggc caggttgcgt tccggtcggt tccatgtgcc
     2701 atccgcacag atgacgatcc tcttcacttg ctccctcccc gggtttcgtt gatacccgtt
     2761 cg
//
LOCUS       DBNHQR010000528         7329 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_305880, whole genome shotgun sequence.
ACCESSION   DBNHQR010000528 DBNHQR010000000
VERSION     DBNHQR010000528.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7329)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7329)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7329
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_305880"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..1604)
                     /locus_tag="AC16VR_12025"
     CDS             complement(30..1604)
                     /locus_tag="AC16VR_12025"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing
                     sulfotransferase family protein"
                     /protein_id="HIR6011928.1"
                     /translation="MVKTADLGALLRDGLGALNAGELERAGAICKQVLARQPNLVQGH
                     FLVGLVALEAKDRKTAFQAFGSVTKLDPAHSAAWAQLARLFMSEGQVNRADAALAEAT
                     QHEPNDPLVHDVIGTVYSLMGEYGLAGEWFEKACATRPDHPPFMLNLANNHVYHGRTP
                     QALALLERVVALQPDSPQAHWSLAGSRTARDDDHIRACARLLMRPGLHPRLQAFYYYA
                     IGKECEDLERWDDAFEAFARGAAARRSTVEFDEAAEVEMFAYLEQQFDESWLESAPAG
                     HPDASPIFVLGQPRTGTTLVERIISSHSEVTSAGELQQFGLGIRRLSGHRDPKRFSTG
                     LFEASLAVDPKQLGGVYLDTTRRMRGDTPRFVDKLPQNYLCIPLILAALPNAKIVHLT
                     RDPMDACFASFKQLFADAYLHSYDQREMARHHARYRRLMDVWRERFPGRFLDISYEET
                     AADLEPNVRRLLAFLDLPFEEACVSFHSQASAVSTASAVQVREPAHTRSVGRWKRYER
                     QLEPMREALAGAGVVG"
     gene            1693..3063
                     /locus_tag="AC16VR_12030"
     CDS             1693..3063
                     /locus_tag="AC16VR_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009395600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HIR6011929.1"
                     /translation="MPIQSRELPEELDRTRHLHPFTDYAAYARSGGRVYTRGEGIFIY
                     DAQGRELLDGMSGLWCTSLGYSQPKIVDAVTEQLQRLPYYNSFFNCSNDAAVAMANAL
                     VEVTPPHFNHVFFTNSGSEANDTNIRLVHRYFDLIGKPDKKIILSRENAYHGSTIAAA
                     SLGGMSSMHKQFTRLPYVHHVQQPYWFELGSGRSPEEFGRQAAQAVADRIDELGADNV
                     AAFIAEPVQGAGGVIIPPETYWPAVQRILDERDVLLISDEVICGFGRTGRWFGCDYFG
                     TRPELLTFAKAVTNGYQPLGGVMVGDRIADVLTTQGGEFAHGFTYSGHPACCAAGLAT
                     LELYHELDTLSYVADDIAPYWASRWSELAAHPIVGESRTLGMLAAVELVSDKSGHTRL
                     DSDGKAALFCRDEAIGGGLMVRAVQDSIISAAPLVSTREEIDLLIERLTQALNDTARH
                     YGINDA"
     gene            3056..4435
                     /locus_tag="AC16VR_12035"
     CDS             3056..4435
                     /locus_tag="AC16VR_12035"
                     /inference="COORDINATES: protein motif:HMM:NF012544.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR6011930.1"
                     /translation="MHDSPPIERHRSLGQRDVVMYTVSAILLLETLTAAASIGAAAIF
                     WWLFLGVVFFLPFALICAEMGCTYPEQGGIYAWVRDAFGGRWGARASWGYWVNTAVWL
                     PAIYILFAGIFAQLYAPGLSLAGQIAIGIGLTWLTVGLNVVTLDVGKWVPNLGAAVKV
                     LVFLTLIGAASYHVATQGFANPINLETIRPTWSDSVAFIPAIIYGMLGFELVSASSEE
                     IKNPRRNVPRGILISGLIIISLYTLATTAMLAALPADEIDLVEGLVATLHQFFASSGA
                     GEIIVSILGAGALYTFFTNGVTWAMGCNRAAAEAGIEGELPRFFALETRRGTPLGAAV
                     VMGAFSTGVLLLYGGLASNSEDLFWSLFAFSAVIFLLPYLGLTLAFLHARRHDAGRHR
                     PFAVPGGMAIARTCAYVCFAALAASIGLFIYTPGEGIVHPVLWGALVTLAIGELTIRF
                     GEHHPVRAG"
     gene            complement(4448..4663)
                     /locus_tag="AC16VR_12040"
     CDS             complement(4448..4663)
                     /locus_tag="AC16VR_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011931.1"
                     /translation="MAYLFCGANSQTAQSKESFEFTYAELGLGSTVVTGGMALAFADS
                     WAHFCAHLFDQDGLVRERSRLTAWLDG"
     gene            complement(5082..5549)
                     /locus_tag="AC16VR_12045"
     CDS             complement(5082..5549)
                     /locus_tag="AC16VR_12045"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6011932.1"
                     /translation="MSDDEYLRDVHEIRNLKHTYCELADRCCSSSDARLAADEIVQLF
                     LEDGELEVPTEYGGRQVGRAAIHAFWMQQSVVFSFADHLVFNERIDVNGIEARGRWKN
                     VIPATIFVEDKPVAQWILGNYEDEYVKVDGQWRIRSVKVSIKRVFGRDEAGVA"
     gene            complement(6076..6834)
                     /locus_tag="AC16VR_12050"
     CDS             complement(6076..6834)
                     /locus_tag="AC16VR_12050"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6011933.1"
                     /translation="MPDLSGKTFIITGGNGGIGLGMAEGIAAAGAGVAIWGRKEEKNA
                     EALEKLKAYGTPAFAYVCDVSDEMAVIETLKRSTDDLGRLDGLFANAGRGGVGKKFVD
                     MTLEDWRAVMAVNLDGVFLTLREAARVLIEQGEGGSLVAVSSTSAIHGAAGNEAYGTA
                     KTGLNGLVRALAVAFARYGIRVNSLLPGWTKTDLAMSSYQSEKFRTVTTNRTPVRRWA
                     DPAEFREIGAFLADPSLTFHTGQEVCVDGGYTVF"
BASE COUNT         1357 a         2382 c         2220 g         1370 t
ORIGIN      
        1 ccttggctga atacccccaa ggggtaggat cacccgacaa cgccggctcc tgcaagcgct
       61 tcgcgcatgg gctccagctg acgttcgtat cgcttccagc gtcccaccga gcgggtgtga
      121 gccggctcgc ggacctggac ggcgcttgcc gtggagacgg cgctcgcctg gctgtggaag
      181 ctgacgcagg cctcctcgaa gggaagatcc aggaaggcca aaagccgacg cacgttgggc
      241 tcgaggtctg cagcggtctc ctcgtaggag atgtccagaa accttcccgg gaaccgttcc
      301 cgccagacgt ccatcagccg tcggtatcgg gcgtggtgac gggccatctc ccgctggtcg
      361 taagagtgca ggtaggcgtc cgcgaaaagc tgcttgaagc tggcaaagca ggcatccatg
      421 ggatcacggg tcagatgcac gatcttggcg ttgggcagtg cggccagaat caacgggatg
      481 cagagatagt tctggggcag cttgtcaacg aagcgggggg tgtcaccgcg catgcggcgt
      541 gtggtgtcca ggtacacgcc cccgagctgc ttcgggtcca cggccagcga ggcctcaaac
      601 agcccagtcg aaaaacgctt cggatctcta tggcccgaaa gacgccgtat ccccaggccg
      661 aactgctgca gttctccggc ggaggtcacc tctgagtgac tcgatatgat gcgctcgacg
      721 agcgtcgtgc cggtgcgcgg ctggccgagc acgaagatgg gcgaggcgtc cgggtgcccg
      781 gctggggcag actcgagcca ggattcatcg aactgctgct cgaggtaagc gaacatctcg
      841 acttccgcgg cctcatcgaa ctccaccgtg ctgcggcggg ctgcggcgcc ccgggcaaac
      901 gcttcgaacg cgtcgtccca ccgctccaga tcctcgcact ccttgccgat ggcgtagtag
      961 tagaaagcct gcaaccgggg atgcagtccc ggtcgcatga gcaggcgggc acacgccctg
     1021 atgtggtcgt cgtcccgtgc ggtccgagag cctgccaggg accagtgcgc ctggggcgag
     1081 tccggctgca gagcgaccac ccgttccagc agagccagcg cctggggcgt gcgcccgtgg
     1141 tagacgtggt tgttggcgag attgagcatg aaaggggggt gatcggggcg cgtcgcgcag
     1201 gctttctcga accattcccc ggcgagcccg tactcgccca tcaacgagta gaccgtgccg
     1261 atcacgtcat gcacaagtgg gtcgttgggt tcatgttgcg tcgcctccgc gagggcggcg
     1321 tctgcgcggt tcacctgccc ctcgctcatg aacagtcggg ccagctgggc ccaggctgca
     1381 gagtgcgcgg gatcgagctt cgtgaccgat ccaaatgcct gaaatgcggt tttgcgatcc
     1441 ttggcctcca gcgcgaccag accgacgagg aagtggccct gcaccagatt aggctgtcgg
     1501 gcgagcacct gcttgcagat tgccccggct cgttccagct cacccgcatt cagcgcgccc
     1561 agcccgtccc gcagcaatgc gcccagatca gcggtcttga ccatctccct gctcactccc
     1621 ccttttgggg taaggcaaca actcggcaat gatgtcacca tcggattgct cacactaggg
     1681 aaggcccgcg cgatgcccat ccaaagcaga gaattgcccg aggagctcga ccgcacccgg
     1741 cacctgcacc ccttcaccga ctacgcggcc tacgcccgtt ccggcgggcg tgtctacacc
     1801 cggggagagg gtatcttcat ctacgatgca caaggccggg agctcctgga cgggatgtcc
     1861 gggctgtggt gcacgagcct cggctacagc cagccgaaga tcgtcgacgc cgtcaccgag
     1921 cagctgcagc gccttcccta ctacaacagc ttcttcaact gctccaacga tgcggcggtt
     1981 gccatggcca acgccctggt ggaagtgacg cccccgcatt tcaaccacgt gttcttcacg
     2041 aactccggct ccgaggcgaa cgacaccaac atccggctcg tgcaccgcta cttcgacctc
     2101 atcggaaagc ccgacaagaa gatcatcttg agccgggaga acgcctatca cggcagcacc
     2161 atcgcggctg ccagcctcgg cggcatgtcc agcatgcaca agcagttcac tcgtctgccc
     2221 tacgtgcacc acgtccagca gccttactgg ttcgagctgg gatcaggacg atcgccggag
     2281 gagttcggcc ggcaggcggc ccaggccgtg gcggacagga tcgacgagct gggcgcggat
     2341 aacgtggccg cgttcatcgc cgagccggtg cagggggcgg gcggggtcat cattcctccg
     2401 gaaacttact ggccagccgt ccagcgcatt ctggacgaac gcgacgttct gctgatcagc
     2461 gacgaggtga tctgcggttt cggccgcaca gggcgctggt tcggttgcga ctacttcggt
     2521 acccgcccgg agcttctgac cttcgccaag gcagtgacca acggctatca accccttggt
     2581 ggcgtcatgg tcggcgacag gatcgccgac gtgctgacca cccagggcgg cgagttcgcc
     2641 cacggattca cctattccgg ccaccccgcc tgctgcgcgg cgggcctggc gaccctggag
     2701 ctttaccacg agctcgacac gctcagctac gtagccgacg acatcgcccc ctactgggcg
     2761 agccgctggt cggagctcgc cgcgcatccc atcgtcggag agtcccgcac cctggggatg
     2821 ctggcagcgg tagagctggt cagtgacaag tccggccaca cacgcctgga ttcggatgga
     2881 aaggcagcgc ttttctgccg tgacgaggcg atcgggggcg gtctgatggt ccgggccgtc
     2941 caggacagca tcatcagcgc cgcgccgctc gtcagcaccc gcgaggagat cgatctgctg
     3001 atcgaacggc tgacccaggc tttgaacgac accgccaggc actacggcat caacgatgca
     3061 tgactcgccg ccgatcgaaa ggcaccgatc gctgggccag cgcgacgtgg tcatgtacac
     3121 cgtctctgca atccttctcc tggagactct caccgctgcc gcttccatcg gcgcggccgc
     3181 catcttctgg tggctgttcc tcggcgtggt ttttttcctg ccctttgcgc tcatctgcgc
     3241 cgagatgggc tgcacctatc ccgagcaggg cggtatctat gcctgggtgc gggacgcctt
     3301 cggcggtcgc tggggggcca gggccagctg gggctactgg gtcaacacgg ccgtgtggtt
     3361 gcccgccatc tacatcctgt tcgccggcat attcgcgcag ctctacgctc ccggcctctc
     3421 cctggccggg cagatcgcca tcggcatcgg cctcacctgg ctcacggtcg ggctgaacgt
     3481 cgtgaccctg gacgtgggca agtgggtgcc gaatctgggg gcagcggtca aggtcctcgt
     3541 cttccttacc ctgatcggag ccgccagtta ccacgtggcc acgcagggat tcgcgaaccc
     3601 catcaaccta gagaccatcc ggccgacgtg gagcgactcc gtcgccttca tccccgcgat
     3661 catctacggg atgttggggt ttgagctcgt cagcgccagt agcgaggaga tcaagaatcc
     3721 ccgcaggaac gtgccccggg gcatcctgat cagcgggctg atcatcattt ccctgtacac
     3781 cctggcgacc acggcgatgc tggccgcgtt gcccgccgac gagatcgacc tggtggaggg
     3841 cctcgtcgcc acgctgcacc agttcttcgc atccagcggt gcgggcgaaa tcatcgtttc
     3901 gatactcggc gccggggccc tctacacgtt cttcaccaac ggcgtgacct gggccatggg
     3961 gtgtaaccgg gcggccgccg aggccggcat cgagggcgag ctgccgagat tcttcgcgct
     4021 ggaaacgcgc cgcggcacac cgctgggcgc tgccgtggtc atgggggcgt tcagcaccgg
     4081 cgtgcttctg ctctacggcg gcctggcgag caacagcgaa gatctcttct ggtcgctgtt
     4141 cgcgttcagc gccgtgatct tcctgctgcc ctatctcggt ttgacgctcg ccttcttgca
     4201 cgcgcgccgc cacgatgccg gccgccaccg gcccttcgcc gtgccaggcg gcatggccat
     4261 cgccagaacg tgcgcctacg tgtgcttcgc cgccctggcg gcatccatcg gcctgttcat
     4321 ctacaccccc ggcgagggta tcgtccatcc ggtgctgtgg ggcgcgctgg tcaccctggc
     4381 gatcggtgag ttgaccatcc gattcggaga gcatcacccg gtgagagcgg gctagcccga
     4441 gccggcttca cccgtccagc cacgcggtga gtcgcgatct ctcccggacg aggccatcct
     4501 gatcgaagag atgcgcacag aagtgcgccc atgagtccgc gaacgccaaa gccatgccgc
     4561 cggtgaccac ggtggagccc agaccaagct cggcgtaggt gaactcgaag ctctcctttg
     4621 attgcgcggt ttgggaattg gctccacaga acaggtaagc caacgctcag ctcgcatgat
     4681 ggcgcgcgaa gccgatcatt tagacggcca acgcgtattc atgagcggcg gggtacgcaa
     4741 gctgcggggc cagcggatat gacctgcgcc cgtgcaccga ctctaattgg tgagagcggc
     4801 tttcactcac gacaggccag ggatatgggc aggaagcagt gtaagccgga gccgaccatc
     4861 cacatgcggg gggagacggg gatcgagctg gccggcggta tgatcacggg cgaagtgtgc
     4921 cgtgaacttg ggattgccga gcggggtctg cctcttcttc ccgatcgcgg aacggcccgt
     4981 ttgcgacagt caccgattct cagcatctgt ctaacgggcc gagccagcta ttgtggatcc
     5041 ggcttccagc gaatattgac ggtcaatcca ccaatatcgc ttcaggctac tccggcctca
     5101 tcgcggccga acacacgctt gatgctcacc ttgacactcc tgatccgcca ctggccgtcg
     5161 accttcacgt attcatcttc gtagttaccg agtatccatt gagcaactgg cttgtcctcg
     5221 acgaatatcg tcgccggaat cacgttcttc catcgaccac gggcctcaat gccgttcacg
     5281 tcaatgcgct cattgaacac aagatgatcg gcaaagctga acaccacgga ttgctgcatc
     5341 cagaacgcat gaatcgcggc ccgaccgacc tgtcggccac catactcggt cggcacctcc
     5401 agctcaccgt cctccaggaa cagctgaaca atctcgtccg cggccagtcg ggcatcggac
     5461 gatgagcagc aacgatcggc taactcgcaa taggtgtgct tgaggttgcg aatttcatgc
     5521 acatcacgca gatactcgtc gtctgacatg gcgtttcagc cctccttggg aaccgtattc
     5581 ttcggcgaga aaacattgtg gcaacgttcg atggaatcga ggcaacaaat gccaatccag
     5641 gcatgtgggc gcgattcatg ccgaccacac gtcgttggcc tatcaggcaa gagtaggcgc
     5701 gccccaacta aacgccacat cctagaagta gcggattccg ttcgtgtagc cacagaccac
     5761 tgatgcgcat ctcggcagca tgatctccac tggtgcttgc tgagatcggc tgccgggcac
     5821 cactcatcag gaaggttaga agccctttct ccgagaatcc agctcagatc ctcaccgatc
     5881 agggccattc cctcttcccg gtcagcccat gttccacaac tggctctgac ggctctcatt
     5941 gaccggtgct gaaactaaag gtttgcgagc atgatcatca acccgtccac caaggagccg
     6001 actcgacatg aatccgcgcg aaacggagca gctggcagca cgggcgattc aacccagtcg
     6061 ggcggaccat gcgggctaga acaccgtgta gccgccgtcc acgcagactt cctgtccggt
     6121 gtggaacgtg aggctcggat ctgccagaaa agcgccgatc tcccggaact cggcggggtc
     6181 cgcccagcgc ctgacgggcg tccggttggt cgtgacggtg cggaatttct cgctctgata
     6241 actgctcatt gccaggtcgg tctttgtcca gccaggcagc agggaattca cccggattcc
     6301 atagcgggca aatgccaccg ccagggcgcg gaccaggccg ttgagtccgg tcttggccgt
     6361 gccgtaggct tcgttgcctg ccgccccgtg aatggccgaa gtggaggaga cggccaccag
     6421 gctcccgccc tcaccctgct cgatgagcac ccgggccgct tcgcgcagag tcaggaacac
     6481 cccatccagg ttcaccgcca tgaccgcccg ccagtcctcg agggtcatgt cgacgaattt
     6541 cttgcccacg cccccgcggc cggcgttggc gaaaaggcca tcgagccgcc cgagatcgtc
     6601 ggtggatcgc ttcagagtct caatcacagc catctcgtca ctgacgtcgc agacataggc
     6661 gaacgcgggg gttccgtagg ctttgagctt ttcgagcgct tccgcgttct tctcctcctt
     6721 gcgcccccag atggcgacgc ctgccccggc agctgcaatg ccctcggcca tgccgaggcc
     6781 tatgccaccg ttcccgccgg taatgatgaa agtcttcccg ctgagatcag gcatcccggt
     6841 catctcctcc tggacgttca ttgagcatct gttggttccc cgctggcgcg accggaagaa
     6901 tagctaaatc tgaggtcacc tggcgatggc tgggacagag aacggctgcc tgaacgggca
     6961 ggagtagcaa cagagcagac accatcgccg cagttcatca ccaccatgtt tccggcggtt
     7021 gttcttctta tacccgctta tatgtacagt ggatctcatc ttcgagcgac ggaccaacgc
     7081 gacgggctca gaacctcgag atgtagattc ggcgttggta tccgagcacg agtttcatgg
     7141 ccaaatcgat acttggcaga ccttcagact tgccgatgca taaagcgccg tcgttgtcct
     7201 caaaatccaa gcatccggct gatttgaacg atcgccatga cgattagaac gacaaatatg
     7261 acctgcgata ttgtgaaagc gattcgcaga tcgttcatcg ccatcatcat aatcatgatg
     7321 tcctgacca
//
LOCUS       DBNHQR010000529         4207 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_179894, whole genome shotgun sequence.
ACCESSION   DBNHQR010000529 DBNHQR010000000
VERSION     DBNHQR010000529.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_179894"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(247..693)
                     /locus_tag="AC16VR_12055"
     CDS             complement(247..693)
                     /locus_tag="AC16VR_12055"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6011934.1"
                     /translation="MPYKHVLVAVDLSEESTQVLDKAREAADGNGAKLSLLSVVKPLT
                     QVYGGLDMAPIASGAVSFEEEALQRAEAQLKDLGSRYGVADDDVHVLLGAPAHEIREA
                     AKAYAADLIIMGTHGRHGLGLLLGSTANAVLHGLECDVLVVRVHFE"
     gene            874..1203
                     /locus_tag="AC16VR_12060"
     CDS             874..1203
                     /locus_tag="AC16VR_12060"
                     /inference="COORDINATES: protein motif:HMM:NF045308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HIR6011935.1"
                     /translation="MNTQEAKSRLEARRAELTDRQERVARHTRHRDEPLSQDFAEQAV
                     ELENGETLVALDREISQELVQIDHALKRIDADTYLICEGCGEPVGEERLKALPYASRC
                     IDCASER"
     gene            1222..1656
                     /locus_tag="AC16VR_12065"
     CDS             1222..1656
                     /locus_tag="AC16VR_12065"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR6011936.1"
                     /translation="MKEPQLKTAMTPFPYAVELSAPIAQARKLMLEHHVHHLPVTEDH
                     ELKGIITDRDIKLLLGPELGSPDPKELTVEDAYIDDCYAVDMGTPLRQVLTTMAARHI
                     GAAVVTAHGRLAGIFTATDACRVFAELLNAQFGSGSEPPDAA"
     gene            complement(1662..3218)
                     /locus_tag="AC16VR_12070"
     CDS             complement(1662..3218)
                     /locus_tag="AC16VR_12070"
                     /inference="COORDINATES: protein motif:HMM:NF025052.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside
                     phosphotransferase/ATP-binding protein"
                     /protein_id="HIR6011937.1"
                     /translation="MAQPELVEHLLRPEAYPHPCRAVRLVETHISWVFLTGDYAYKVK
                     KPVLFSFVDFSTARLRKHYCEEELRCNRAFSPELYLDVVEIVRDVEGRIRVGGTGEVV
                     EHAVKMRQFPQSSALDRLLVAGVLEPGGLSRFGLELATLHAGLPRLEAPDDELDRRIL
                     GPVLDNFATLRATRAGGDRSDILQRLESASKDAFDGKRTTFSRRLAKGFVRECHGDLH
                     LRNLVMIDGRVRAFDCLEFNPDLRWIDIASDAAFLIMDCAVRDRVDLAYAFQDGYLAG
                     CGDYVGATLSPFYQAYRSVVRAKVAALEIEGSPKDGKLGAARERLDRQLNWAWERLHG
                     PRGRMVLMCGLSGSGKSYVAERLVPRLPAVRIRSDVLRKVAAGLEPLQRSGSSVDAGL
                     YTPSRSQSVYAEMQAIALALAAAGETVIVDATFLERAARKAFRGAAAAAGVPCVVLHC
                     QASEPTMRSRVAAREREGRDASEAGIAVLEAQQSRFEPPTADEETVVVDTETTAVMAQ
                     IEGALRAAFA"
     gene            3390..>4207
                     /locus_tag="AC16VR_12075"
     CDS             3390..>4207
                     /locus_tag="AC16VR_12075"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6011938.1"
                     /translation="MFAVCPLISPMVAHPNTAEQEKTGKELYEQQCARCHQRDGGGVD
                     GLYPSLRNAPSLWLDRKTAIRGVLAGRADDTVVNSPLMPTHGYLGNETVASTLTFLLQ
                     EWGPGGQPYTAEEVAAERLELLASQPAPHPGLPDVSPLADMDAVQYVTSSGPPMSVSE
                     FDRARSLYYGHCTGCHGVLREGTAGSPLTPVLMRQRGTEYLQAVINYGSSAGMFNWGT
                     SAALTAEEINMLARFLQHPVPQAPDMTEADVRASWSLEVPLKDRPGVPQGRHPL"
BASE COUNT          822 a         1406 c         1262 g          717 t
ORIGIN      
        1 gctttgaccg cagaccgtgg acacagtgtt acccaactgg tcggagctga ccaatgaaac
       61 atccgcaagg ttgtgtggat atgttgtgga tcagaccgtc gcaatcctgt ggaaaagttc
      121 acgcaagctt gcccgcgttt gagctcgcgc tgccaagcaa ctgaaagctc gactttttcc
      181 gcacatgcat ctcgttcaat acgcaaggcc tcgagcgcaa cgtcaattgg cgcggtcgcg
      241 tccgcttcac tcgaagtgca cgcgcacgac cagcacgtcg cattccagcc cgtgcagaac
      301 ggcgtttgcc gtggagccga gcaggagccc caggccgtgc cggccatgag tgcccatgat
      361 gatgagatcg gcggcgtaag ccttggccgc ctcacggatc tcgtgcgcgg gcgcgccgag
      421 cagcacatgg acgtcatcgt ccgccacgcc gtaacggctg cccaggtcct tcaactgggc
      481 ttccgctcgc tgcagcgctt cctcctcgaa ggaaaccgcg ccgctggcaa tgggggccat
      541 gtccagacca ccgtagactt gagtcagcgg ctttaccacg ctcaagagag acagtttagc
      601 cccgttgccg tccgcggctt cccgagcctt gtcgagtacc tgcgtcgact cttcggacag
      661 atctaccgcc accagcacgt gtttgtacgg catcgggcca cctcccgtga tcatttctcc
      721 ttgagattag cccaccatcg cagcgagagc catccttgtc ggtaccggga acttcggcga
      781 tcctctattc ggaaactgcg taaggacggc gggcagcgag aggcggtatg atctacccga
      841 acacgttcaa gccggacgaa ctcggagcat acagtgaaca cacaagaagc caaatcgagg
      901 ctggaggcgc gccgggcgga actcacggac cggcaggagc gcgttgcccg ccacaccaga
      961 caccgggacg agccgctgtc gcaggatttc gccgagcagg cggtggagct ggaaaacgga
     1021 gagaccctcg tcgctctgga cagggagatc agccaggagc tcgttcagat cgatcacgcc
     1081 ctgaaacgaa ttgatgcaga cacctatctg atttgcgagg gatgcgggga gcccgtcggt
     1141 gaggagcgac tgaaggctct gccctatgcc agccgctgca tcgactgcgc cagcgagcgc
     1201 tgaaccggat ccggcagcct ggtgaaggaa ccgcagctca aaacggccat gacgcctttc
     1261 ccctacgccg tcgagctctc ggctccgatc gcccaggccc gaaagctcat gctcgaacac
     1321 catgtccatc atcttccggt cacggaagat cacgagctca aaggcatcat taccgaccgg
     1381 gacatcaagc ttctgctcgg ccccgaactc ggctccccgg atcccaagga gcttaccgta
     1441 gaggatgcct acatcgacga ctgctacgcc gtcgacatgg ggacacctct gcggcaggtg
     1501 ctgactacca tggctgcgcg gcacatcggc gcggccgtgg tcaccgctca cgggcgactt
     1561 gccggaatct tcaccgccac cgatgcgtgc cgcgttttcg cggagcttct caacgcccag
     1621 ttcgggtccg gcagcgaacc accggacgct gcctgatagg ctcaggcaaa cgccgcccga
     1681 agggccccct cgatttgcgc catcacggct gtggtttccg tgtcgaccac gaccgtttcc
     1741 tcatccgctg tgggcggctc aaatcgcgac tgctgcgcct ctagcaccgc gatgccggcc
     1801 tcggaggcat ccctgccctc ccgctcccgg gctgccaccc gtgacctcat cgtgggttcg
     1861 gatgcctggc aatgcaggac gacgcagggc acgcccgccg ccgccgcggc gccgcggaaa
     1921 gcctttcttg ccgcgcgctc gagaaaagtg gcgtccacga tgaccgtttc gcctgcagca
     1981 gctaaagcca gcgcgattgc ctgcatctcg gcgtaaaccg attggctccg tgaaggcgtg
     2041 tacagccccg catccaccga tgatccggat cgttgcagcg gttccaggcc ggctgccacc
     2101 ttgcggagca cgtcagatcg gatacgcaca gcgggcaatc gcggcacgag ccgctccgct
     2161 acatacgatt ttccagagcc ggatagaccg cacatcaaaa ccatcctgcc ccggggccca
     2221 tgcagacgct cccaggccca gttcagttgc cggtcgagac gttcccgggc agcgcccagc
     2281 ttgccgtcct tcggcgatcc ttcgatctcc agggcagcca ccttggctcg gacgacggat
     2341 cggtaggcct gataaaaagg ggacaacgtc gccccgacgt aatctccgca gccggccagg
     2401 tagccgtcct gaaaagcgta ggccaggtca acgcgatctc gcaccgcaca gtccatgatc
     2461 aggaaagcgg cgtcgcttgc gatgtcgatc cagcgcaggt cggggttgaa ctcgagacag
     2521 tcgaacgccc tcacccgccc gtcgatcatg acgagattac gcaggtgcag atcgccgtga
     2581 cactctcgca cgaagccctt ggccaggcgg cgcgagaacg ttgttcgctt gccgtcaaaa
     2641 gcgtccttcg aggccgactc gagcctttgg aggatgtccg agcgatcccc gccggcccgg
     2701 gttgccctga gcgtcgcgaa gttgtccagc accggcccca ggatccgccg gtccagctcg
     2761 tcgtccgggg cctccagacg tggcagccca gcatgcagag tggccagctc cagtccgaat
     2821 cgactcaacc caccgggctc gagaacgcct gcgaccagaa gccgatccag cgcgctgctc
     2881 tgaggaaact gccgcatctt cactgcatgc tcaacgacct cgccagtccc gccaacccgg
     2941 atccggccct cgacatcccg gacgatctcg accacgtcca gatagagctc cggagagaac
     3001 gcccggttgc agcggagttc ctcctcgcaa tagtgtttcc tgagacgagc ggtgctgaaa
     3061 tccacaaagc tgaagaggac cggtttcttg actttgtagg cgtaatctcc cgtgaggaag
     3121 acccaggaga tgtgcgtctc gaccaggcga acggctctac aggggtgcgg gtaagcctcg
     3181 ggacgtagca gatgctcgac gagctcgggc tgggccactc tcaacacctc gccgaagcgg
     3241 ggctgacgtc cgagcctgcc gccagatgct cactagggga actacggata ggcaaatcgt
     3301 cgccgaaaac cgttagcatt gacacgacca tgccatgccg ggaaaccctc ttgacgccag
     3361 ctaaacccat taaggccacg ttgatcggct tgtttgccgt ctgcccgctc atctctccga
     3421 tggtagccca ccccaatacg gcagaacagg aaaagacggg caaggagctc tacgagcagc
     3481 agtgcgcccg atgccaccag cgcgacgggg gcggcgtaga cgggctctat ccgagcctga
     3541 gaaatgctcc ctccctttgg ctcgaccgga aaacggcgat tcgtggggtg cttgccggac
     3601 gcgctgacga caccgtggtc aattcccccc tcatgccgac ccacggctat ctcggcaacg
     3661 agaccgtcgc cagtaccctg acgttcctgc tgcaggaatg ggggccgggg gggcagcctt
     3721 acaccgccga ggaggtcgcc gctgagcgac tcgagctgct ggcaagccaa ccggcccccc
     3781 acccgggact gcccgatgtg agcccgctgg ccgatatgga cgcggtgcag tacgtgacgt
     3841 cgagcggccc tcccatgtcc gtgagcgagt tcgaccgggc gcgatctctg tactacgggc
     3901 actgcacggg ttgtcacggc gtgctccgcg aaggtactgc gggatcgcca ttgacgccgg
     3961 tgctgatgcg ccaacgcggg accgaatacc ttcaggcggt catcaactac ggttcatccg
     4021 cgggaatgtt caactggggc acctcggccg cgctgacagc cgaggagatc aacatgctcg
     4081 ctcgctttct gcagcacccg gtgcctcagg ctcccgacat gaccgaggca gacgtgcgcg
     4141 cgagctggtc tttggaggta cctctaaagg accgtccagg ggtgccccag gggcgccatc
     4201 cgctgga
//
LOCUS       DBNHQR010000530         4130 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_115720, whole genome shotgun sequence.
ACCESSION   DBNHQR010000530 DBNHQR010000000
VERSION     DBNHQR010000530.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4130)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_115720"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1282
                     /locus_tag="AC16VR_12080"
     CDS             <1..1282
                     /locus_tag="AC16VR_12080"
                     /inference="COORDINATES: protein motif:HMM:NF023284.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3376 domain-containing protein"
                     /protein_id="HIR6011939.1"
                     /translation="CIVYIEPDPSAVDVVSPERNLGYLRTIRAALSSIPRNQPIVDDL
                     QAIVSQDKRVRINRRIVESNAAEINTLVRELQDVARRQEMTADLITYLRTAILERAEE
                     DMSIAYHAYVQRRVWRLTDALVDEWAVLAENPNDERTRDGMSESISRSWQGAESGDAG
                     RRDNMQETFLDRFDVTYRIRRLQFVIRRINQHEDVTELDEASTDALDSFKNDAYGFLE
                     RLHRLRRSQYLDGPLIEKLAVAARNLPLEPDEARDLLKDIFGALGLQSFDREFDALIC
                     TFLDRLTDDRLRDQMLTDYVGFPVFDVLLMSPASLEGGPDPLTPISVERISPEDAPTL
                     RSVFKGLKSRQFMGFLGFFNRAYREHDYLWGRLNAADRLVDLLVQSSRDAISDPDRMR
                     LKLFRAVVDRERQQLYRCDDELDRIDAFLKAAGV"
     gene            1300..2667
                     /locus_tag="AC16VR_12085"
     CDS             1300..2667
                     /locus_tag="AC16VR_12085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR6011940.1"
                     /translation="MSSGVDLSAGPVSRHLVNLTIPMFLGISSMILASMIDTVYVGWI
                     GTRELAAVSFSFPVVLAMATVSMGIGVGAASIIARVVGAGDMERVRRLSTNALYLVAM
                     LVLSLTLLGMLFVEPLFRVLGAGSDILPLSVRYMTVWFLGLPAFALPMVATNIMRAVG
                     SARLPGVIMAAGAGLQVLIAPPLIFGIPGVADGLGITGAAWSFVLSRLAMFGYTVRVM
                     QRMNLLSFAREPWSSRRESWGEVLKIGLPSMVGNLIGPVSMGVVVALLADYGHEVVGG
                     FGVASRIESLATMIIMALAASTGPVIGQNWGARRYERIYRAQSLGFRFSFLWSLLAFA
                     LLAGFGRPIVGFINQDPAVVDATYLYLLLLPATYGLLGVGMVASSTFIALGKPMPTLV
                     LSLLRMIVLYIPMALIMETLFGYAGIFAAAAVCNCLFGGLAYGWVRRMLHKEISRHGR
                     YEGAG"
     gene            2668..>4130
                     /locus_tag="AC16VR_12090"
     CDS             2668..>4130
                     /locus_tag="AC16VR_12090"
                     /inference="COORDINATES: protein motif:HMM:NF023167.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane phospholipid transport protein
                     YdbH family protein"
                     /protein_id="HIR6011941.1"
                     /translation="MKWLLAIVILLAGLLAGAYASLPWLAETLALREAAAVGIEVREL
                     DVRFPRWGEIAVDRLVASMQGNELRAEQLSVAYNWSGVRERRLAFVGAALLTVYVEDS
                     SGDAEPGALGEQIVVLPGLFLDAWQELPVESVQVDEVRLRTGELVFAGTFGFTPAAAS
                     LAGEVSGVEPLGALRLSMRADAAGSVGLAIQQLERPANSLAVSADARRLAGEAVIAWN
                     LELPVEDGELAVTLEGRGPIELSENGWRLGSGLQARVDGGAWRGGFAVAEAEGPWPED
                     EATELVRVSGAWQLEADLPHDVAARGTLSAGLDRSGAKLSIDPGMVVTGAAVSGASWS
                     LEGIRLDTLDGLALEVGIEPMTLTLGSPVRAALAADAARWENFALAPYVDLRIDSLAM
                     DPGSARGAFAATVPALQLSGSGQIRADAESSTMVVELSARQRLRAPLLATFLAGWDQP
                     YDVDAGNVAVTGSLTLGDGALSGDVVVTLEGVQSHYG"
BASE COUNT          595 a         1313 c         1413 g          809 t
ORIGIN      
        1 ctgcatcgtc tacatcgagc ccgatccgag cgccgtggac gtcgtgagcc cggagcgtaa
       61 cctgggatac ctccgcacga tccgcgcggc gctctcctcg attccccgca accagcccat
      121 cgtagacgat ctccaggcca tcgtgtccca ggacaagcgg gtgcgcatca accggcgcat
      181 cgtcgaatcc aatgcggccg agatcaatac cctggtccgc gagctgcagg acgtggccag
      241 gcgccaggag atgacggcag atctgatcac ctacctgcgg acggctattt tggagcgcgc
      301 cgaggaggac atgagcattg cctatcacgc ctacgtgcag cggcgcgtct ggcggctgac
      361 cgatgcgctc gtcgacgagt gggcggtgct tgccgagaac cccaacgacg agcgaacgcg
      421 ggatggcatg agcgagtcca tatcgcgctc gtggcagggg gcagagtccg gggatgccgg
      481 ccggcgcgac aacatgcagg aaacgttcct cgaccgattc gacgtcacct accgcatccg
      541 gcgcctgcag ttcgtcatcc ggcggatcaa tcagcacgag gacgtcacgg agctggatga
      601 agcgtccacc gacgcgctgg atagcttcaa gaacgacgcc tacgggttcc tcgagcgcct
      661 gcatcgactg cggcgatccc aatacctcga cggcccgctg atcgaaaagc tcgcggtggc
      721 cgcccggaac ctgccgctcg agccggacga ggccagggat ctactcaagg acatattcgg
      781 cgcgctgggg ctgcagtcgt tcgaccgcga gttcgatgcc ctgatctgca ccttcctcga
      841 ccggcttacc gacgaccggc tgcgcgacca gatgctcacg gattacgtcg gctttccggt
      901 tttcgacgtg ctgctcatgt cgccggcgtc cctggagggc ggccccgacc cgctcacgcc
      961 catcagcgtg gagaggatca gcccggagga cgcgccgacg ctcaggtcgg tcttcaaagg
     1021 actgaagtcg cgccagttca tgggttttct cggtttcttc aaccgggcct atcgcgagca
     1081 cgattacctc tggggcaggc tcaacgccgc ggaccggctg gtcgatctgc tcgttcagtc
     1141 gtctcgtgat gccatcagcg accccgatcg gatgcgcctc aaactctttc gcgccgtcgt
     1201 cgaccgcgag cgtcagcagc tgtaccggtg cgatgacgag ctcgacagga ttgatgcgtt
     1261 tctgaaagcc gcgggcgtct agcggatgag accggattcg tgagctcggg cgttgacctg
     1321 tccgcgggcc ccgtatcccg ccacctggtc aacctgacga tccccatgtt cctcgggatc
     1381 tcgtcgatga tcctcgcgtc gatgatcgat acggtctacg tcggctggat cggcacccgg
     1441 gagctggcgg cggtgagctt ctcctttccc gtggtcctgg ccatggcgac ggtctccatg
     1501 ggaatcgggg tcggggcggc gtccatcatc gcccgggtgg tgggggcggg cgacatggaa
     1561 cgggtgcggc gcctgtcgac gaacgcgctc tacctggtcg ccatgctggt attgagcctc
     1621 accctgctcg ggatgctttt cgtcgagccg ctcttcaggg tgctgggcgc agggtccgac
     1681 atcctgcctc tgtcggtgcg ctacatgacg gtgtggtttc tcggcctgcc ggcttttgcg
     1741 ctgcccatgg ttgccaccaa catcatgcgc gcggtgggca gcgcgcgcct gccgggcgtc
     1801 atcatggccg cgggtgccgg ccttcaggtg ctcatcgcgc cgccgctgat cttcggtatc
     1861 cccggcgtgg cggacgggct gggaatcacc ggggcggctt ggtcattcgt gctgtctcgg
     1921 ctcgccatgt tcggctacac ggtcagggtg atgcagcgca tgaacctgtt gtcgttcgcc
     1981 cgtgagccct ggtcttcgcg tcgcgagtcc tggggggaag ttcttaagat cggcctgccg
     2041 tccatggtcg gcaacctcat cggcccggtc tccatgggcg tggtggtggc cctgctggcc
     2101 gactacgggc acgaggtggt gggcgggttt ggcgtcgcct cgcgcatcga gtccctggct
     2161 accatgatca tcatggccct ggctgccagc acgggaccgg tgattggcca gaactggggt
     2221 gctcgccgct acgagcgcat ctaccgggcg caatccctgg ggttccgctt cagcttcctg
     2281 tggtcactgc tggctttcgc cctcctcgcc ggtttcggcc ggcccatcgt gggcttcatc
     2341 aaccaggacc cggccgtcgt cgatgcaacc tacctctacc tgttgttgct tccagctacc
     2401 tacggccttc tcggtgtggg catggtggcg agctcgacct tcatcgcgtt gggcaagccc
     2461 atgcccaccc tggtgctttc gctgttgcga atgatcgttc tctacatacc aatggctttg
     2521 atcatggaaa cgctcttcgg ctatgcggga atctttgccg cggcggccgt ctgcaactgc
     2581 ttgttcggcg gcctggccta tggttgggtg cgccggatgc tgcacaagga gatttcccgt
     2641 catgggcgct acgaaggggc cgggtaggtg aagtggctcc tggcaatcgt gattcttctg
     2701 gccggcctgc tggcaggcgc ttacgcgtcg ctgccctggc tggccgagac gctggccctg
     2761 cgcgaggcgg ccgctgtcgg catcgaggtg cgggagctgg acgtgcgatt tccgcgctgg
     2821 ggtgaaattg ccgtggatcg gctcgtcgcg agcatgcagg gcaatgagct gcgggccgag
     2881 caactgtccg tcgcttataa ctggtcgggc gtccgggagc gaaggctggc gttcgtgggc
     2941 gctgcgctgc tgacggtgta cgtcgaggac tccagcggcg atgcggaacc cggggcgctt
     3001 ggagagcaga tcgttgtgct gccgggcctg ttcctggacg cctggcagga actgccggtg
     3061 gagagcgttc aggtggatga ggtccgtttg cgcacgggag agctcgtctt tgccggtacg
     3121 tttggattca cgccagcggc agccagcctg gcaggcgagg tgagcggcgt cgagcccctt
     3181 ggcgcgcttc ggctttccat gcgcgcagat gccgcgggct cggttggcct ggccatccag
     3241 cagctagagc gaccggccaa ctccctggct gtctcggccg atgctcggcg tctggcgggc
     3301 gaggcggtta ttgcctggaa tctggagctg cccgttgagg atggcgaact cgccgtcacc
     3361 ctggaaggcc ggggccccat cgagcttagc gagaatggct ggcggttggg atccgggctg
     3421 caggcgcgcg tggacggcgg ggcttggcgc ggaggcttcg cggtggccga agcggagggc
     3481 ccctggcctg aagacgaggc taccgagctc gtccgtgtct ccggcgcctg gcagctcgaa
     3541 gctgacctgc cccatgacgt tgcggcgcgt ggaaccctgt cggccgggtt ggacagatcc
     3601 ggcgcgaagc tgtccatcga cccgggcatg gtggtgaccg gtgccgccgt ttcgggtgcg
     3661 tcttggagtc tcgagggtat ccgtctggat accctcgacg gccttgcgct cgaagtcggg
     3721 atcgaaccaa tgacgctcac cctggggagc ccggttcgcg ccgcgctggc tgcggatgcc
     3781 gcgcgctggg agaacttcgc acttgctccc tacgtcgacc ttcgcatcga ttccctcgcc
     3841 atggaccccg gcagcgcgcg gggcgcgttt gcggcaacgg tccccgccct ccagctctcc
     3901 ggctctgggc agattcgtgc ggacgccgag tcgtcgacca tggtcgtcga gctatcggct
     3961 cgccagcggc tgagagcgcc gctgctggcc acgttccttg ctggctggga tcagccttac
     4021 gacgtcgacg cgggaaacgt cgcagtgacg ggcagcctga ccctcggtga cggggcgctg
     4081 tcgggcgacg tggtcgtgac cctcgaaggc gttcaatccc actacggcga
//
LOCUS       DBNHQR010000531         3263 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1445535, whole genome shotgun sequence.
ACCESSION   DBNHQR010000531 DBNHQR010000000
VERSION     DBNHQR010000531.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1445535"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1452
                     /locus_tag="AC16VR_12095"
     CDS             <1..1452
                     /locus_tag="AC16VR_12095"
                     /inference="COORDINATES: protein motif:HMM:NF014585.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HIR6011942.1"
                     /translation="TLRPGDVLLTNDPWDGTGHLNDFTAVTPVFLADACVGLFASTSH
                     IADVGGRGFGPDANDLFEEGLRIPITFLFREGVRDETLMMLVAANVRDPVVAEGDLYS
                     LTACNDTGARQLVELMDDFGLADLEEVGREILEKSRRAMMAEIRNLPPGEYRHTMRID
                     GYDRPVDLACTLTVSGSGIDIDWTGTSPVSAAGINVPLPYTEAYASFGVRCVVGNDVP
                     NNAGSLDTIRVTAPRGCILNAEPPRAVSARHAVGQMLPDVVLGCLAQVVSDRVPAEGA
                     SCIWNPVFLNAPSEDGGNAPELQFVTNPIYNGGTGARPGKDGLSTTAFPSGVRTTSTE
                     VNEATAPLLIWRKEYRQDSAGPGEHRGGLGQTVEVAHARGESFVISKMFDRIRHPARG
                     RNGGGDGEPGRVYVKGGDDFRGKGRDVVPPGATLVMETPGGAGMGRVAERDPDRIAAD
                     VEAGLVSPDAARDRYGLDAGVRQNDDEGGAADV"
     gene            1523..1598
                     /locus_tag="AC16VR_12100"
     tRNA            1523..1598
                     /locus_tag="AC16VR_12100"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1556..1558,aa:Gly,seq:gcc)
     gene            1931..2158
                     /locus_tag="AC16VR_12105"
     CDS             1931..2158
                     /locus_tag="AC16VR_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011943.1"
                     /translation="MLEFAVVKQGHARNKLLQTARSIFYIEVSPENQVAVTDLFRSCD
                     YDIFHLQGDASEQRVQECTFYTVARPRDAAG"
     gene            complement(2488..2931)
                     /locus_tag="AC16VR_12110"
     CDS             complement(2488..2931)
                     /locus_tag="AC16VR_12110"
                     /inference="COORDINATES: protein motif:HMM:NF016955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HIR6011944.1"
                     /translation="MRNRPFLEASMAASALIAMADEHVNAAERLSIESVMENIRRLNV
                     FEPRTAIRLHCQFVAAIRANAATGEALALRTISRFKGDLKAGEMLVNIAHYTAHADNE
                     LVPEELVAIRRVYRELGIDPAHHEPAIEYEEPDGPRSRTYRLRTA"
BASE COUNT          549 a         1016 c         1093 g          605 t
ORIGIN      
        1 acgctgcggc cgggtgacgt gctgctcacc aacgacccct gggacggaac cggtcacctc
       61 aacgatttca cggcagtcac gccggtcttt ctcgcggatg cgtgcgtagg cctgtttgcg
      121 tccaccagcc acatcgcgga tgtcggggga cgaggcttcg ggcccgatgc caacgacctg
      181 ttcgaggaag ggctgcgcat tcccatcact tttctgttcc gggagggggt gcgcgacgag
      241 acgctcatga tgctcgtcgc cgcgaacgtg cgagatccgg tcgtggcaga aggcgacctc
      301 tattcgctga ccgcctgcaa cgacacgggc gcgcgccagc tcgtggagct gatggacgac
      361 ttcgggctgg ccgacctgga ggaggtgggg cgcgagatac tcgaaaagtc ccgtcgtgcc
      421 atgatggccg agatccgcaa ccttccgccg ggcgagtacc ggcacaccat gcggatagac
      481 ggttacgaca ggcccgtgga tctggcctgc acgctgacgg tcagcggcag cgggatcgac
      541 atcgactgga cgggcacctc gcccgtttcc gcggcgggca tcaacgtgcc tctaccctac
      601 accgaggcct atgcgtcatt cggcgtgcgc tgcgtggtgg gcaacgacgt gccgaacaac
      661 gcgggttccc tggacaccat ccgggtcacc gctccccgag gctgcatcct caacgcggag
      721 ccgccgcgcg cggtctcggc gcgtcacgcc gtaggtcaga tgttgccgga cgtggtgctc
      781 ggctgccttg cccaggtggt ttcggaccgg gtgccggcgg agggcgcgtc gtgtatctgg
      841 aaccccgtgt tcctgaacgc tccctcggag gacgggggca acgcaccgga gctgcagttc
      901 gttaccaacc ccatctacaa cggaggcacc ggggctcggc cgggcaagga tggtctttcc
      961 accacggctt tcccgtcggg ggtgcgcacc acgtcgacgg aggtcaacga agcaacggcg
     1021 ccgctgctga tatggcgcaa ggagtatcgg caggactcgg ctggtccggg ggagcatcgc
     1081 ggcgggctgg ggcagacggt ggaggttgcg catgcgcggg gagaatcttt cgtgatctcc
     1141 aagatgttcg atcgcatccg gcatcctgcc cggggtcgaa atggcggggg agacggcgag
     1201 cctggtcggg tctacgtgaa agggggagat gacttccggg gcaagggcag ggacgtggtg
     1261 ccaccaggcg ccacgctggt catggagacc ccgggcgggg ccggcatggg cagggtggcc
     1321 gagcgcgacc ctgaccggat cgccgccgat gtcgaggccg gtctggtcag ccccgatgca
     1381 gcgcgcgatc gctacgggct cgacgccggg gtccggcaga atgacgatga aggcggcgcg
     1441 gcagacgtct agcgcttcca ctcggcgggc cgattcggct atagttcgcg tccggccgtc
     1501 ggaccggacg gattcttcca gggcgggaat agctcagttg gtagagcaca accttgccaa
     1561 ggttggggtc gcgagttcga gtctcgtttc ccgctccact tcatttcgac gcgttcgtcg
     1621 attctttctc ccatgcacgc cgatgatgtt gatgagaggg cggtttcccg ggacggtcga
     1681 ggaaccatga atttccgcaa cctcgtcaat ttcgacttca gtaaacgttt gcccagggag
     1741 ttcgggggcg agcttatgta cgtcacggcg cggtccgaca tccgcgtaca gggttggtcc
     1801 gcagggcgcc atttacgcgc tggaagccga tccggcttac gcagatcgga taatccgctc
     1861 gagccgccgc ctgtcatcgg cctaccagcc ggtcaacgtt ctgtgcgccg ccatcgccga
     1921 tcacgcgggg gtgctggagt tcgctgttgt gaaacagggg cacgcacgga acaagctgct
     1981 ccagaccgcg cggtcgatct tctatatcga ggtgtctccg gaaaaccagg tggcagtcac
     2041 cgatctgttc cgaagctgtg actacgacat cttccacctt cagggtgacg cctccgagca
     2101 gcgggtgcag gagtgcacct tctacacggt ggcccggccc cgcgatgctg ccgggtaacc
     2161 cgtccggcaa gggcgcttcg gcagcgccgt gacgggagat cacggcatcg cctcggatcg
     2221 atggctgctc gccgagcccg cgcagatcgg ctgcacctac ggctgatccg cgcggttgcc
     2281 gagcttctcg gcgtcgcggt gaccgaactg aggctgaccc aatctgactt ctggcctaag
     2341 tacggcgccg gggaccgacc gccgttctgg gtttcccgca gcccggttcc ccctactgga
     2401 cgacggcgac catcgatgcc gttgaggccc gctacggcgt attcggaatg aacatgacgc
     2461 cgtatcggca ggcgctggat tcctgactca ggcggtgcgc aggcggtatg tgcgcgaacg
     2521 cgggccgtcg ggttcctcgt actcgatggc tggttcgtgg tgagccggat cgatgccgag
     2581 ttccctgtag acgcgacgaa tggcgaccag ttcctccggc acgagctcgt tgtcggcgtg
     2641 tgccgtgtag tgggcgatgt tcaccagcat ctcgccggcc ttcaggtcgc ccttgaagcg
     2701 ggagatggtg cgcagcgcca gcgcttcgcc cgtagcagcg ttggccctga tcgcagctac
     2761 gaactgacag tgaagccgaa tcgcggtgcg tggttcgaag acgttgagcc gtcggatgtt
     2821 ctccatcacg ctctcgatgg acaggcgctc cgcggcgttc acgtgctcgt cggccatggc
     2881 gatcagcgcg ctggctgcca tggaagcctc gaggaacgga cgatttctca atcgtacgta
     2941 accgtctccg gcgatcttct ccctcaaact cactatcgac tccgcttccc tcgaacgtgg
     3001 gcgccggcat tgaatgctct gttctacgcc cagagcgatc gaaccatggg gccgatgcac
     3061 ctgccacgaa tcagttcaca agaggcgggc acatcccatg gcaatggatc aaaaccccga
     3121 ccgcgtatta cggcaagcgt gaagtgtgtt caagttttcc ctacgggaag cgccacgacg
     3181 ttggattgcg atggctgcag tttgatccat cggccgaccg ccgcgcgtaa ctcaccgtcg
     3241 ctgagcggcg tcatcaattt tcg
//
LOCUS       DBNHQR010000532         3621 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1508782, whole genome shotgun sequence.
ACCESSION   DBNHQR010000532 DBNHQR010000000
VERSION     DBNHQR010000532.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1508782"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="AC16VR_12115"
     CDS             complement(<1..252)
                     /locus_tag="AC16VR_12115"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6011945.1"
                     /translation="MSRVLISGASRGIGLELVKQYAAAGDEVIACVRDTGNAALLDEV
                     ASGADNVTVQQMDAGDPASIEAAAAAIGDAPIDILINNAG"
     gene            complement(280..693)
                     /locus_tag="AC16VR_12120"
     CDS             complement(280..693)
                     /locus_tag="AC16VR_12120"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybH family protein"
                     /protein_id="HIR6011946.1"
                     /translation="MTSSADDIAAIEKLQADWNAAVEAGSVDGYAAVLDDDIELLPTD
                     AETIRGIGNYRAMLHGVFTTDDFRIEIAVPATIEVDGDMAWSRYDYVIHRTAKDGSGT
                     FSSYRKFLDVMRRQDDGTWRVYKHIWNYNDPDVVP"
     gene            complement(735..1703)
                     /locus_tag="AC16VR_12125"
     CDS             complement(735..1703)
                     /locus_tag="AC16VR_12125"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HIR6011947.1"
                     /translation="MAQTVTLTRRWPAQVEAHLQSLYDLSVDTTDRPRTRDELAAALA
                     SADALCPTVTDRIDADLLGSAEVRTRIIASFGVGHEHIDLDAARARGIVVTNTPGVLT
                     DCTADLTLALLLGIARRIGEGEREVRGGSWTGWRPTHMMGTRVTGKTLGIVGLGRIGV
                     AVARRARLGFGMRVLFHNPTPPPPERIAAAGAEPCSTLVELLQRSDFVSLHCPATADT
                     HHLINAERLAQMPAHAYLINTARGTVVDSEALATALRERRIRGAGLDVYEGEPEVPAG
                     LLELDNVILLPHLGSATEETRTAMGMRVAENLAAFFAGSEPPDRLV"
     gene            1783..2394
                     /locus_tag="AC16VR_12130"
     CDS             1783..2394
                     /locus_tag="AC16VR_12130"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6011948.1"
                     /translation="MRLHESPSPNARRVHVFLKEKGIEAPPTVRVDIRGGENLSDAYR
                     KLNPFGRVPVLELDDGTCLAESVAICRYFEGLHPEPSLFGREPLEMAIIEMWNRRAEI
                     NFMLQATGAFRNLTGFFNDREKVSKEWGAIALGNAADALGRFDTALGTSRFLAGETFS
                     IADITFAVVLQFAQMVKLDLPFDLPNIERYLAEVGSRPSFQES"
     gene            2459..3235
                     /locus_tag="AC16VR_12135"
     CDS             2459..3235
                     /locus_tag="AC16VR_12135"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6011949.1"
                     /translation="MKLEGKVAAITGGTRGIGRGIAEAFLAEGANVVVNGRTPEKGQR
                     ALEEMGVGDAAHFIAGDVRVQADVEAFVDGTIERYGKIDILVNNAGGSSGFAPISELS
                     DEAWNEALDWMLNATFWGTRRALPDMLSRKWGRIINISSVEGLLINKTTVGHYIVNKH
                     AINALTKVVAYENGPDGITSNAILPGAVETDLMTITGAEAAKSLGMTYEQYIDGYAQE
                     AAIKRINTVEEVAAVALLLASDLGGGITGALMEVHGGTAL"
     gene            3268..>3621
                     /locus_tag="AC16VR_12140"
     CDS             3268..>3621
                     /locus_tag="AC16VR_12140"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6011950.1"
                     /translation="MAAARSLPYPNRAYAWFVLAVLVVAALIAFIDRQVVAIVVDPMK
                     ADLGVGDAQIGWLYGVFALFYAVAALPIAWLADRKSRKHIIAAGIFLWSLMTIACGLS
                     RSYWNVFLARIGVGIG"
BASE COUNT          597 a         1156 c         1216 g          652 t
ORIGIN      
        1 gccggcgttg ttgatcagga tgtcgatggg ggcatcgccg atggcagcag ccgccgcttc
       61 gatggaggcc gggtcgccgg cgtccatctg ctgaacggtg acgttgtccg cgccgctggc
      121 aacctcgtca agcagggcgg cattgcccgt gtcccgcacg cacgcgatca cctcgtcgcc
      181 ggccgccgcg tactgcttca ccagctccag tccaattccc cggctcgcgc cgcttatcag
      241 tactctcgac atatttgagt cctccctctt cgaatttccc tagggtacta catcgggatc
      301 gttgtagttc cagatatgtt tgtacacccg ccaggtgccg tcgtcctggc gtcgcatgac
      361 gtccaggaac ttccggtagc tgctgaacgt cccgctgccg tccttggccg tgcggtgaat
      421 gacgtagtcg tagcgggacc aggccatgtc gccgtccacc tcgatggtgg cggggaccgc
      481 gatctcgatg cggaagtcgt cggtggtgaa caccccgtgg agcatggccc ggtagttgcc
      541 gatgcccctg atggtttccg catcggtggg cagcagctcg atgtcgtcgt ccagcacggc
      601 ggcgtaaccg tcgacgctgc cggcctcgac ggccgcgttc cagtcggcct ggagcttctc
      661 aatggcggcg atgtcgtccg ctgatgacgt catggttccc ccaacacgtt gcctgtgcga
      721 agcgcgaaag tttatcagac aagccgatcc ggaggctcgc tccctgcgaa gaacgcggcc
      781 agattttcgg cgacccgcat gcccatggcg gtgcgcgtct cctcggtggc gctgcccaga
      841 tgcggcagca ggatgacgtt gtcgagctcg agcaggccgg cgggtacctc cggttcgccc
      901 tcgtagacgt cgagcccggc gccgcggatt cggcgctcgc gcagcgccgt cgccagggct
      961 tcggaatcca ccaccgtgcc tcgggccgtg ttgatgagat aggcgtgggc cggcatctgt
     1021 gcaagccgct cagcgttgat cagatggtgg gtgtcggcgg tggctgggca gtgcagcgac
     1081 acgaagtcgg agcgttgcag caactcgaca agggtggagc atggctcggc gccggcagcc
     1141 gcgattcgtt ccggtggtgg cggcgtcggg ttgtggaaga gcacgcgcat cccgaaaccc
     1201 agccgggcgc gccgggccac ggctacgccg attctgccga gcccgacgat gcccagcgtc
     1261 ttgcccgtca cccgcgtgcc catcatgtgt gtcggacgcc agcccgtcca ggacccgcct
     1321 cgaacctccc gttcgccctc gccgatacgg cgggcgatac cgagcagcag cgccagggtg
     1381 agatcggccg tgcagtcggt cagcactccg ggcgtgttgg tgaccacgat cccccgggcc
     1441 cgggcggcat ccaggtcgat gtgctcgtga ccgacgccga agctcgcgat gattctcgtt
     1501 cgcacctcag ctgagcccag caggtcggca tcgatccggt ccgtcacggt ggggcagagc
     1561 gcgtccgcgc tagcaagcgc ggcagccagc tcgtcgcggg tgcgcggtcg gtcggtagtg
     1621 tctaccgaga ggtcgtaaag cgactggaga tgggcctcca cctgcgcagg ccagcgccgg
     1681 gtgagcgtga ctgtctgggc catatgcttt gtcctcggaa gcgaatcatg gtattgcaca
     1741 tcgcgtacaa ttgtccgcat cgctgaagcg gggagagttc tgatgaggct gcacgagtcg
     1801 ccatcgccaa atgcgcgccg ggtccacgtt ttcctgaaag agaagggcat cgaagcgccg
     1861 cccacggtga gggtggacat ccgggggggc gaaaatctca gcgacgccta ccgcaagctc
     1921 aaccccttcg gccgggttcc cgtgctggag ctggatgacg gcacctgcct ggcggaatcc
     1981 gttgccatct gtcgctactt cgaggggttg catccggagc cctcgctgtt cggccgggag
     2041 cctctggaga tggcgatcat cgagatgtgg aaccgccggg ccgagatcaa cttcatgctt
     2101 caggcaaccg gcgcgttccg aaacctgacc ggcttcttca atgaccgtga gaaggtctcc
     2161 aaggaatggg gcgccatcgc gctgggcaac gccgcagatg cgctcgggcg cttcgacacc
     2221 gcgctcggca cttcccggtt cctggccggc gagacgttca gcatcgccga catcaccttt
     2281 gcggtggtgc tgcagtttgc ccagatggtc aagctggacc tgccttttga tctgccgaac
     2341 atcgagcgat atctggcaga ggtgggatcg cgacccagct tccaggagag ctgatctcag
     2401 cgcgtcgccc tagcggcggc cagaggggta tggtcaaacg acgggaggaa accgacggat
     2461 gaaactggaa ggcaaagtgg ccgccatcac tggcgggacg aggggcatcg gccgcggcat
     2521 cgcggaggcc tttctggccg agggggcgaa tgtcgtcgtg aatgggcgaa cgcccgaaaa
     2581 gggccagcgt gccctggagg agatgggagt cggagacgcc gcgcacttca tcgccggcga
     2641 cgttcgcgtt caggccgatg tggaagcctt cgtggacggc accatcgagc gctacggcaa
     2701 gatcgacatt ctggtgaaca acgccggcgg ctcatccggc tttgcaccga tttccgagct
     2761 cagcgacgag gcgtggaacg aagcgctgga ctggatgctc aacgcgactt tctggggcac
     2821 gcgacgggcg ctgcccgaca tgctgtcccg caagtggggc cggatcatca acatctcctc
     2881 cgtggagggg ctgctcatca acaagacgac ggtgggccac tacatcgtca acaagcacgc
     2941 catcaacgcg ctcaccaagg tggtggccta cgagaacggt cccgacggga tcacctccaa
     3001 cgccatcctg ccgggcgctg tggaaaccga cctgatgacc atcaccggtg ccgaagccgc
     3061 caagtccctc ggcatgacct acgagcagta catcgacggc tacgcccagg aggcggccat
     3121 caagcggatc aataccgtgg aggaagtcgc ggcggtggcc cttctgctgg cctccgacct
     3181 gggcggcggc atcaccggtg cgctcatgga agtgcatggg ggcaccgcgc tctgagcacg
     3241 ccctcgggag ccggggaacg cggcgcattg gccgccgcgc gttctctgcc gtatccgaac
     3301 cgggcctacg cgtggttcgt gctggccgtt ctggtggtcg ccgcgctcat tgccttcatc
     3361 gaccggcagg tggtcgccat cgtggtggat cccatgaagg cggacctggg cgtgggagac
     3421 gcccagatcg gttggctgta cggcgttttc gcgctcttct acgcggttgc cgcgctcccc
     3481 atcgcctggc tggccgaccg caagagccgc aagcacatca tcgcggccgg catctttctt
     3541 tggtccctga tgaccattgc ctgcgggctg tcgcgcagct actggaacgt gttcctggcc
     3601 cgcatcgggg tgggcatcgg c
//
LOCUS       DBNHQR010000533         3235 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_686721, whole genome shotgun sequence.
ACCESSION   DBNHQR010000533 DBNHQR010000000
VERSION     DBNHQR010000533.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_686721"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="AC16VR_12145"
     CDS             complement(<1..480)
                     /locus_tag="AC16VR_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6011951.1"
                     /translation="MPEYDRSAEDVGNVQMLEHVNVTVPDQGLAALFYVTGLGFTRDP
                     YIDFGTHNMWVNLGEQQFHLPQSDAQILRGEIAVVVPNLEDLAKRLKFVSKALKDTRF
                     SWEEADQRVVLSCPWGNRIVAYPAGSFPAMDLGMPYVDMSVPPGTAPGIARFYESVMG
                     "
     gene            591..1691
                     /locus_tag="AC16VR_12150"
     CDS             591..1691
                     /locus_tag="AC16VR_12150"
                     /inference="COORDINATES: protein motif:HMM:NF014305.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011952.1"
                     /translation="MGQDTDVLLEATMALVPPLLTALDALAHAGRHLHPQSLAHLVPQ
                     IDAFARPLRQGARKFEEIPWPPHLERFRDHISASAGEALAAMDAMSACLEQANPVLAA
                     YQAMGAATRATERLYPVSFMLPPVSRFFLTEAGRDDGALARRLERADPGRDHVGVMHA
                     HNGPGERGGFSLYVPEYYDAGEAWPVVVALHGGSGHGRSFLWNWLKDARSRGVLLVAP
                     TSRDRTWSLAGSDADSASLDAIVERVCEHWRVDERRILLTGMSDGGTFSYLSGLRSDT
                     PFTHLSPISASFHPFILEGVDRDRLTGLPIYLVHGTLDWMFPVDVARTARDALTAAGA
                     ALTYREVDDLSHTYPREENARILDWLMGPSAP"
     gene            complement(1696..2331)
                     /locus_tag="AC16VR_12155"
     CDS             complement(1696..2331)
                     /locus_tag="AC16VR_12155"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6011953.1"
                     /translation="MLTLWGGATSRTLRAHWMLHELGLDYEPRLIGSRTGATQTDAFK
                     QLNPKEKIPVLVDDEFTLTESVAIVTYLGDTYGSGSGLVPEPFTRDRARYNEWASFIQ
                     MELDAHTLYVLRKHRDLAGIYGEAPAAVATAIGGFAKQVAVADEALRGSAHLIGSKFS
                     AADLLLVSCLDWAAAYGLELTPGLDEYRLRIRKRPAYRAAARLNFSISAGA"
     gene            complement(2338..2640)
                     /locus_tag="AC16VR_12160"
     CDS             complement(2338..2640)
                     /locus_tag="AC16VR_12160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HIR6011954.1"
                     /translation="MIIVNAIIESDENSIAAMREGITTMVTATLEEPGCDDYCFSVEL
                     DDPTRIRITERWHSIEALAAHFQTPHMAAFQQVMAANPPKNVTAHCYDAEEIDLPR"
     gene            complement(2637..>3235)
                     /locus_tag="AC16VR_12165"
     CDS             complement(2637..>3235)
                     /locus_tag="AC16VR_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar-transfer associated ATP-grasp
                     domain-containing protein"
                     /protein_id="HIR6011955.1"
                     /translation="SLGGRADVAILESLVRVSPLFRNVSYEGVPDIRLIVFQGYPVMG
                     MLRLATLASDGKANLHQGAVGVGLDIGTGKSVAAVQHNRIVRNHPDTGAELTELSVPN
                     WRQLLELAARCFEVTQLGYLGCDIVIDELRGPLLLELNARPGLSIQLANDAGLVPRLR
                     HIENLESFAHNVEDRVDYAMETFTRERRTEQTKLWEGQ"
BASE COUNT          516 a         1064 c         1065 g          590 t
ORIGIN      
        1 gcccatcacc gattcgtaga accgtgcgat gccaggggcg gttccgggcg ggacggacat
       61 gtccacgtag ggcattccca ggtccatggc gggaaaagat ccggcggggt aggcgacgat
      121 gcgattgccc cagggacagc tgagcaccac cctttggtct gcctcttccc acgagaagcg
      181 ggtgtccttc aacgccttgg agacgaactt cagtcttttc gcgagatctt cgaggtttgg
      241 aacgaccact gcgatctcgc cgcgaaggat ctgggcgtcc gactggggga gatgaaactg
      301 ctgctcaccg agattgaccc acatgttgtg cgtgccgaag tcgatgtacg ggtcgcgggt
      361 gaagccgaga ccggtcacgt agaacagcgc ggccagcccc tgatcgggaa cggtcacgtt
      421 gacgtgctcc agcatctgca cgttgccgac gtcctcggcg ctgcggtcgt actcgggcaa
      481 ttgacgatct tcgagtggcg caacccatga ctttaatcaa tctgccgacg ccgatccaac
      541 gcggcgggcg ccggcgctca gccggtccat cggcagggta tagtgcgctg atgggccaag
      601 acaccgacgt cctgctcgaa gcaaccatgg cgctggttcc accgctgctc accgcgctgg
      661 atgccctcgc ccatgcgggc aggcacctgc atcctcagtc cctggcgcat ctggttcctc
      721 agatcgacgc gttcgcccgg ccgctccggc agggcgcgcg caagttcgaa gagatcccgt
      781 ggccgcccca cctggagcgg tttcgcgatc acatcagcgc ttctgcgggt gaagcgctcg
      841 cggccatgga cgcgatgagc gcgtgcctag agcaggcgaa cccggtgctc gccgcctacc
      901 aggccatggg tgccgcgacc cgcgctaccg aacgcctgta cccggtctcc ttcatgctcc
      961 ccccggtcag ccgctttttc ctgaccgagg ccggccgcga cgacggcgcg ctggcgagac
     1021 gcctggagcg ggcggatcct ggccgggacc acgtgggggt gatgcatgcc cacaacggac
     1081 ctggtgagcg gggcggtttt tccctctacg ttccggagta ctacgacgcc ggcgaggcgt
     1141 ggcctgtggt ggtcgccctg cacggcggca gcggtcacgg gcgcagcttt ctctggaact
     1201 ggttgaagga tgcccgctca cggggcgtgc tcctggtcgc gcccacctcc cgcgaccgaa
     1261 cgtggtcgct ggcaggctcc gatgcggaca gcgcgagcct ggacgccatc gtcgaacggg
     1321 tctgcgagca ttggcgcgtg gacgagcgcc gcatactgct cacgggcatg tccgacggcg
     1381 gaaccttcag ctatctgagc ggcctgcgct cggatacccc gttcacccat ctctcgccca
     1441 tctcggccag ctttcatccc ttcattctgg aaggcgtgga tcgggatcgc ctgaccggcc
     1501 tgcccatata cctcgtgcac gggacgctgg actggatgtt ccccgtcgac gtggcgcgca
     1561 cggcgcgcga tgccttgacc gcagctggag ccgcgctcac ctaccgcgag gtcgacgatc
     1621 tatcccacac ctatccccgg gaggagaacg cccgcatcct ggactggctg atggggcctt
     1681 cggctccctg agggctcagg ctccggcaga tatagagaag ttgagccggg ccgcagcccg
     1741 gtaagcgggc cgcttgcgga tccgcaaccg gtactcgtcg aggccgggcg tgagctcgag
     1801 cccgtaggcg gcggcccagt ccaggcagct gaccagcagc aggtctgctg cgctgaactt
     1861 gctgccgatc agatgagccg accccctgag cgcttcgtct gccacggcca cctgcttggc
     1921 aaacccgcca atggcggtag ccaccgcggc gggggcttcg ccgtagatgc ctgccaggtc
     1981 ccggtgtttg cgcaggacgt agagcgtgtg cgcgtcgagc tccatctgga tgaagctggc
     2041 ccattcgttg tagcgcgccc ggtcccgggt gaaaggctca ggtacgagac cgctgccgga
     2101 gccgtaggtg tcgcccaggt acgtgacgat ggccacgctc tcggtgagcg tgaattcgtc
     2161 gtccaccagc acgggtatct tctccttggg gttgagctgc ttgaaggcgt ctgtctgggt
     2221 ggcgcccgtg cgcgagccga tcaggcgggg ctcgtagtcg agccccagct cgtgcagcat
     2281 ccagtgcgcc cgcagcgtgc ggctggtcgc gcccccccaa agcgtcagca cgacggctca
     2341 gcgcggcagg tcgatttctt cggcgtcgta gcagtgggcg gtgacgttct tcggcgggtt
     2401 ggcggccatc acctgctgaa acgccgccat gtggggcgtc tgaaaatggg cggccagcgc
     2461 ttcgatgctg tgccagcgct cggtgattcg gatcctggtc ggatcgtcga gctccacgct
     2521 gaagcaatag tcgtcgcatc cgggctcctc gagcgtggcc gtcaccatgg tggtgatgcc
     2581 ttcgcgcatg gcggcgatgc tgttttcgtc cgactcgatg atggcgttga cgatgatcat
     2641 tgtccctccc agagtttcgt ctgttccgtt ctgcgctctc gagtgaaggt ctccatggcg
     2701 tagtcgacgc gatcctcgac gttgtgcgcg aaagactcga gattctctat gtgtcgcagg
     2761 cgcggcacca gccccgcgtc gttggccagc tggatgctca gccccggccg agcgttcagc
     2821 tccagcagca gcggcccgcg aagctcgtcg atgacgatgt cgcagcctag gtagcccagc
     2881 tgtgtcacct cgaagcatcg ggcagccagc tccagaagct ggcgccagtt cggaacgctc
     2941 agctcggtca gctcggcccc tgtatcgggg tgattgcgaa cgatccggtt gtgctgcacg
     3001 gcggccacgc tcttgcccgt gccgatgtcg agcccgaccc ctaccgcacc ctgatgcaga
     3061 ttcgccttgc cgtcggaggc cagagtggcc aggcgcagca tgcccatgac cggatagccc
     3121 tgaaagacga tcagccggat gtctggaaca ccctcgtagc tcacgttccg gaacagcggg
     3181 ctgacgcgca ccagagattc cagaatagcc acatcggcgc gcccgcccag ggagt
//
LOCUS       DBNHQR010000534         4168 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_307128, whole genome shotgun sequence.
ACCESSION   DBNHQR010000534 DBNHQR010000000
VERSION     DBNHQR010000534.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4168)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_307128"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /gene="cobS"
                     /locus_tag="AC16VR_12170"
     CDS             <1..918
                     /gene="cobS"
                     /locus_tag="AC16VR_12170"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobS"
                     /protein_id="HIR6011956.1"
                     /translation="DFQVPAFSKRSDYVPEVDVDYRFDPDTTLAILAGFAHNRRVMIA
                     GYHGTGKSTHIEQVAARLNWPCIRVNLDSHISRIDLIGKDAIVLEEGKQVTEFREGIL
                     PWAIQHPVALVFDEYDAGRPDVMFVIQRVLEVEGKLTLLDQNKVITPHPFFRLFSTTN
                     TIGLGDTTGLYHGTQQINQAQMDRWSIVTNLNYLEHDAEVDIVLGKAKHYAETEDGAR
                     TISNMVAVADLTRKGFVNGDVSIVMSPRTVITWAENAEIFGDIGYAFRVTFLNKCDEL
                     EQPIVAEYYQRCMGEDLGYATAGIALKSA"
     gene            935..2746
                     /gene="cobT"
                     /locus_tag="AC16VR_12175"
     CDS             935..2746
                     /gene="cobT"
                     /locus_tag="AC16VR_12175"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobT"
                     /protein_id="HIR6011957.1"
                     /translation="MSEEEHPLEPFKRATTATIRAIAENDELEVTFGQGPPTVRGNRI
                     RVPLPAVGSAPQEVDAIRGMGDEFALRIRYHDDDVHRSRAPRSGPAQEMFQWIEDARI
                     ASIGSLRMEGVAQNLDASLEVQCQQAAFDTITAEADAPLSVAVGLIVRQHLTGRDLPP
                     SAENVVRFWRAYVEENAGDDIDALRDCLLDQAEFAERCRAIIADLGLGAELDEPPELD
                     ESQDDTDAMDEDQDADSDISPDDVVLDDDSMMDESAEGDATMMEMDADMDMDELGAEM
                     EPDEAPTQMPDESGRIRMDFNYEAYTQEFDETVRAEELCDADEMIRLRALLDQQLVPL
                     QHATSKLANRLQRKLMAKQSRTWEFDLDEGMLDASRLSQVIIDPMNPLAYKQEKEMQF
                     RDTVVTMLIDSSGSMRGRSITIAAMCADILGRTLERCSVRVEVLGFTTRAWRGGQSRE
                     KWIASGKPSNPGRLNDLRHIIYKAADDPWQRTRRNLGLMMREELLKENIDGEALIWAH
                     NRLVVRHEERKVLMVISDGLPVDNSTLLVNPSNYLEQHLKYAIDQIENYSSVELIAIG
                     IGHDVTHHYQRAVTITDAEQLGGAMTEQLAELFEAKR"
     gene            complement(2805..>4168)
                     /locus_tag="AC16VR_12180"
     CDS             complement(2805..>4168)
                     /locus_tag="AC16VR_12180"
                     /inference="COORDINATES: protein motif:HMM:NF012361.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR6011958.1"
                     /translation="MGIEIVRVVAGEGDGADTPLAEAYTGDGVLVNSGPFDGLGVADG
                     KRAVTAWLGERGLGELKVNYRLRDWCISRQRYWGPPIPIVHCDACGAVAVPEDQLPVE
                     LPRVDDFKPDDTGVSPLARVASWYETTCPGCDGPARRETDVSDTFLDSSWYFLRYPST
                     DRNDAPMDDGITARWLPVDSYIGGNEHAVLHLMYSRFINMVLKDLGHIDFEEPFVKFR
                     AHGLITRNGAKMSKSRGNVITPDAIIDQYGADTFRTYLMFMGPLEEGGDYRDEGIQGP
                     FGFLHRLWDAVVTAKPDTAADEDVERKLHKTIKQATEQIPELGYNTAIAAMMECLNAV
                     RAGGRQPAVAEVEPLVVMIAPFAPHLAEELWERLGHGKSIFAGANWPAYDEAKVRDEV
                     VSVAVQVNGKLRGTISLAPGTDQAEAEAVARAEENVARHLDNAEVRRVIYVPNRLLNF
                     VVG"
BASE COUNT          743 a         1389 c         1372 g          664 t
ORIGIN      
        1 gacttccagg tgccggcgtt cagcaagcga tcggactacg tgccggaggt ggacgtggat
       61 taccgtttcg atccggacac cacgcttgcg atcctcgctg gtttcgccca caaccgccgg
      121 gtgatgattg ccggctacca cggcacgggt aagtccaccc acatcgagca ggtggccgcg
      181 cggctgaact ggccgtgcat ccgcgtgaac ctcgacagcc acatcagccg gatcgacctg
      241 atcggcaagg acgccatcgt gctggaggag ggcaagcagg tcaccgagtt ccgcgaaggc
      301 atcctgccct gggcgatcca gcatccggtg gcgctggtat tcgacgagta cgacgcgggc
      361 cgccccgacg tgatgttcgt cattcagcgg gtgctggagg tcgaaggaaa gctcaccctt
      421 ctcgaccaga acaaggtcat cacgccccat ccgttcttcc gtctcttctc caccaccaac
      481 accatcggtc ttggcgacac caccgggctg taccacggca cccagcagat caaccaggcc
      541 cagatggacc gctggagcat cgtgaccaac ctcaactacc tcgagcacga tgcggaggtg
      601 gatatcgtgc tgggcaaggc caagcactac gcggagaccg aggacggcgc gcgcacgatc
      661 tcaaacatgg tggcggtggc cgatctcacc cgcaaaggct tcgtcaacgg cgacgtctcc
      721 atcgtgatgt ccccgcggac ggtgatcacc tgggcggaga atgcggagat cttcggagac
      781 atcggttacg ccttccgcgt gacgttcctg aacaagtgcg acgagctcga gcagcccatc
      841 gtggccgagt actaccagcg ctgcatgggc gaggatctgg gttacgccac ggcgggaatt
      901 gcgctcaagt cggcttgagt cctgacaagc gccgatgtct gaggaagaac acccgctaga
      961 gccgttcaag agggccacca cagccaccat ccgggccatc gcggagaacg acgagctgga
     1021 ggtcaccttc ggccaggggc cgcccaccgt gcgcggcaac cggatccggg tgccccttcc
     1081 ggcggtgggg tccgcaccgc aggaggtgga cgccatccgc ggcatgggtg acgagttcgc
     1141 cctgcgcatc cgctatcacg acgatgacgt gcatcgcagc cgcgcgccgc gatcgggccc
     1201 ggcccaggaa atgttccagt ggatcgagga tgcccgcatc gcctccatcg gctccctgcg
     1261 tatggagggt gtcgcccaga acctggatgc aagcctggaa gtgcagtgcc agcaggcggc
     1321 cttcgacacc atcaccgctg aggctgacgc ccccttgagc gtcgccgtcg ggctcatcgt
     1381 ccgccagcac ctcacgggcc gcgacctgcc gccgtcggcc gagaacgtgg tccgtttctg
     1441 gcgcgcctac gtcgaggaga acgcgggcga cgacatcgac gccctgcgcg attgcctgct
     1501 cgaccaggcg gagtttgccg agcgctgtcg ggccatcatc gccgacctcg gcctgggtgc
     1561 cgagctcgac gagccgccgg aactcgacga gagccaggac gataccgacg ccatggacga
     1621 ggaccaggac gcggattcgg acatcagtcc ggacgacgtg gttctcgacg atgactccat
     1681 gatggacgag tccgccgagg gcgacgccac catgatggag atggacgccg acatggacat
     1741 ggacgagctc ggcgcggaga tggagccgga cgaagcgccc acgcagatgc ccgacgagag
     1801 cggtcggatc cgcatggact tcaactacga ggcctacacc caggagttcg acgagaccgt
     1861 ccgcgccgag gagctgtgcg acgccgacga gatgatccgg ctccgcgcgc tgctcgacca
     1921 gcagctcgtc cccctgcagc atgccacctc caagctcgcc aaccggctgc agcgcaagct
     1981 catggccaag cagagccgga cctgggagtt cgatctggac gaggggatgc tggacgcctc
     2041 gcggctatcc caggtgatca tcgatcccat gaacccgctg gcctacaagc aggaaaagga
     2101 gatgcagttt cgcgacacgg ttgtcacgat gctcatcgac agctccggat ccatgcgcgg
     2161 acggtccatc accatcgccg ccatgtgcgc ggacattctg ggccgcaccc tcgagcgctg
     2221 ctccgtgcgg gtggaggtgc tcggtttcac cacgcgagcc tggcggggcg gccagtcccg
     2281 ggagaagtgg atcgcctcgg gcaagccgtc gaacccgggg cgcctgaacg atctccgcca
     2341 catcatctac aaggcggccg acgacccctg gcagcgcacg cgccgcaatc tcggcctcat
     2401 gatgcgcgag gagctgttga aggagaacat cgacggcgag gcgctcatct gggcccacaa
     2461 tcgcctggtg gtgcgtcacg aggagcgcaa ggtgctcatg gtcatctccg acggcctgcc
     2521 ggtggacaac tccacgctgc tggtgaaccc gagcaactac ctagagcagc acctgaagta
     2581 cgccatcgac cagatcgaga actactcgtc ggtggagctc atcgccatcg gcatcgggca
     2641 tgacgtgact caccattacc agcgcgcggt caccatcacg gatgcggagc agctcggcgg
     2701 tgccatgacc gagcagctgg cggagctctt cgaggccaag cgctgacggg ccgggccctg
     2761 ggaagccgac ttcaaacggg agcctgcggt ccccgacacg acgactaacc gaccacgaag
     2821 ttcagcaggc ggttgggcac gtagatgacg cgacgcactt cggcgttgtc cagatggcgg
     2881 gccacgttct cctcggcccg ggccaccgcc tcggcttcag cctggtcggt gccgggtgca
     2941 aggctgatgg tgccgcgcag cttgccgttg acctgcacgg ccaccgagac cacctcgtcg
     3001 cggaccttgg cctcgtcgta ggccggccag tttgcgcccg cgaagatgct cttgccgtgt
     3061 ccgaggcgct cccacagctc ctcggccagg tggggggcaa agggcgcgat cataaccacc
     3121 agcggctcca cctccgccac tgccggttgc ctgccgccgg cgcgcacggc gttcaggcac
     3181 tccatcatgg cggcgatggc cgtgttgtaa ccgagctcgg gaatctgctc ggtggcctgc
     3241 ttgatggtct tgtgcagctt gcgctccacg tcctcgtccg cagcggtgtc gggcttggcg
     3301 gtcacaaccg catcccacag ccggtgcagg aacccgaagg ggccctggat gccctcgtcc
     3361 cggtagtcgc cgccttcctc cagggggccc atgaacatga ggtaggtgcg aaaggtgtcc
     3421 gcgccgtact ggtcgatgat ggcgtccggg gtgatgacgt tgccccggga tttggacatc
     3481 ttcgcgccgt tgcgggtgat gagcccgtgg gcgcggaact tcacgaaggg ttcctcgaaa
     3541 tcgatgtggc cgaggtcctt gagcaccatg ttgatgaaac gcgagtacat caggtgcagc
     3601 acggcgtgct cgttgccgcc gatgtagctg tccaccggca gccagcgggc ggtgatcccg
     3661 tcgtccatgg gcgcgtcgtt ccggtccgtg gacgggtagc gcaggaagta ccaggaggag
     3721 tccaggaagg tgtcggacac gtcggtttcg cggcgcgccg ggccatcgca gccggggcag
     3781 gtggtttcgt accagctggc cacccgggcg agcgggctca cgccggtgtc gtcgggcttg
     3841 aagtcgtcga ctctgggcag ctccaccggc agctgatcct caggcaccgc caccgcgccg
     3901 caggcatcgc agtgcacgat ggggatgggc ggtccccagt agcgctgccg ggagatgcac
     3961 cagtcccgga gccgatagtt gaccttgagc tcgcctaggc cccgctcgcc cagccacgcg
     4021 gtgaccgcgc gcttgccgtc agccacgccg agcccgtcga aggggccgga attcaccagc
     4081 acgccgtcgc cggtgtatgc ctcggccagc ggcgtgtcgg ccccgtcgcc ttccccggcc
     4141 accacgcgga ctatctcgat gcccatgg
//
LOCUS       DBNHQR010000535         4758 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_560650, whole genome shotgun sequence.
ACCESSION   DBNHQR010000535 DBNHQR010000000
VERSION     DBNHQR010000535.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4758)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_560650"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="AC16VR_12185"
     CDS             complement(<1..385)
                     /locus_tag="AC16VR_12185"
                     /inference="COORDINATES: protein motif:HMM:NF039185.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HIR6011959.1"
                     /translation="MIGRALRALFPPICLLCDQSCAAEADVDLCSRCLPVLPWNPQPC
                     PRCALPRENGTGRCQPCEDDPLPVARTVAPLLHAGYPRHWVHRLKFHEGMVEGRLLAA
                     LLADAVRQRYAEADLPDVLVPVPAPP"
     gene            592..2358
                     /locus_tag="AC16VR_12190"
     CDS             592..2358
                     /locus_tag="AC16VR_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014590723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HIR6011960.1"
                     /translation="MPASLKDVRFLIHDVFDFAGHYARLELDEPPGRDLIDAIVEEAA
                     RFADGELAPLNRVGDESGCRLEDGSVSTPAGFKEAYAKYVEGGWAGLSGSAAYGGQGL
                     PDSMSFFLEDLMCCANMAWTMYPGLSRGVVGALEEHGSAELKDAFLANLLSGEWTGTM
                     CLTEPHAGSDVGLARTRAEASADGTYRISGTKIFISAGEHDMAENIVHLVLARIPDAP
                     PGTKGISMFVVPKFDLDGTRNGVSCGSIEEKMGIHGNATCVLNFDGARGYLVGEPNHG
                     MRYMFTMMNGARLVVGLQGASQAHAACLLSLEYAQDRLQMRSLTGPKAPERPADPIIV
                     HPDVRRMLLFQRAIAEGGRALVYYAAQIIDRFRDGSPDERASAGELLDFLTPVVKGIL
                     TELGFESVNQAVQIYGGHGFIRETGVEQYVRDTRITTIYEGTTQIRALDLVGRKILQN
                     QGKGLVSFIGEMQAVAASLEDPLPRLAAPLKALADEWGELTLQLSATAMQDLDEVGAA
                     AVDYLYYSGYAALAFCWALMARSADEAIRAGSTDPFHAGKIAVADFFFSRVLPRAKAH
                     QAAIEAGKGTLMDVDEGALVAL"
     gene            2407..4143
                     /locus_tag="AC16VR_12195"
     CDS             2407..4143
                     /locus_tag="AC16VR_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015502313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HIR6011961.1"
                     /translation="MLEYQAPLRDFDFLLFDVFRAGETWGRIPAFAELSEELVRAVLA
                     EGGRVAREVMAPLNQTGDEQGCRWDDGVVTAPAGFKAAYDDMAGGGWLGISGNPEFEG
                     QGMPKMLGCLVEEMFWSANTSLFLYGTLSVGASICIDTHGSAAQKSLYLPKLYSGRWS
                     GAMDLTEPHAGSDLGMMRTRAESAGDGTYRITGTKIFITSGEHDLSENIVHLVLAKVP
                     GAPAGSRGISLFIVPKFLPDADGEPGERNALASGSIEHKMGIRGSATSVMNFDGAVGF
                     LVGEENQGLAAMFTMMNYERLSVGLQGLGAGELAYQQAARYATERIQGRSPEGPQKPE
                     AAADPLIVHPDVRRMLLTIRCLTEAGRAFGLFAGMQLDLGKHLGDPDALALGDLLTPI
                     AKAFLSDRGLDCAVLGQQVFGGHGYVREWGMEQIVRDARIAQIYEGTNGIQAQDLIGR
                     KILRDGGAVLRRLATIVEADARDSAYRADLLAAVARLVKVTDSLVQRSRADAALPGAV
                     AVDYLDLAGYTLYAWLWARMADAAPGDDFGRSKRACAEFYFKRLLPRTLGLAAAIEAE
                     SEAVMALAADAF"
     gene            complement(4140..>4758)
                     /locus_tag="AC16VR_12200"
     CDS             complement(4140..>4758)
                     /locus_tag="AC16VR_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011962.1"
                     /translation="LAGCDTGSGAEDTAQAQARTGAEPASPELLMARPPEGWKVWSAT
                     QDPGLRVAQYVPADTDADNWEQMLRLESMAGDPLPDPIEFLTALGRDQAAGCGQSSDL
                     NIHSGQENNYATSVRLFSCRNDAAPDLDQVTLIKAIRGNENFYVIALTRRTTSGESTR
                     PPVSDAEMAVWAAYMRTVTVCDPTRPEHPCPTSGEAPEQPAATAG"
BASE COUNT          771 a         1498 c         1656 g          833 t
ORIGIN      
        1 ctggaggagc gggaacgggc accagcacgt ccggaagatc ggcctctgcg tagcgctgcc
       61 ggaccgcatc ggccagcagg gccgccagca accggccctc gaccatgccc tcgtgaaact
      121 tgagccggtg gacccagtgg cgcgggtagc cggcgtggag cagcggcgcc acggtgcggg
      181 ccaccggcag agggtcgtct tcgcagggtt ggcatcgccc ggtgccgttc tcccgcggaa
      241 gcgcgcagcg gggacagggc tgcgggttcc agggcaggac gggcaggcac cggctgcaca
      301 ggtctacgtc tgcctcggct gcgcagctct gatcgcagag caggcagatg ggcgggaaca
      361 gggctcgcaa cgcgcgaccg atcatcggtt gatcctgccc gttggacgtt tggcacgaca
      421 gtagcgaatg tcctcgtatg gcgtgcgaag cccgtcacga ttcacccgct cctccgggcg
      481 ggagcaggcc gcgattttgc gcatcagcgc ggggcgaacg cgatctgtgg agggggtcat
      541 tgacgggccg tgaagatttg ctaccgtgcc ctccgcattg gaggatccaa gatgccagca
      601 tcgctcaaag acgtgcggtt tttgattcac gacgtgttcg atttcgccgg gcactacgca
      661 cggctcgagc tcgacgaacc accgggccgc gatctcatcg acgccatcgt cgaggaagct
      721 gcgcggtttg ccgatggcga gctggcgccg ctcaaccgcg ttggggacga atccggctgc
      781 cgactggaag acgggtcggt gagcacgccg gccgggttca aggaggccta cgccaagtac
      841 gtcgaaggag gctgggccgg tctcagcggt tccgctgcct acggtggcca gggcctgccg
      901 gacagcatga gctttttcct ggaggacctg atgtgctgcg cgaacatggc ctggaccatg
      961 tacccgggcc tttcgcgggg ggtcgtcggc gctttagagg agcacgggag cgcggagctc
     1021 aaggacgcgt ttctcgccaa tctcctctcg ggtgagtgga cggggaccat gtgtctgacc
     1081 gagccccatg ccgggtcgga cgtgggcctg gccagaaccc gggcggaggc ctctgcagac
     1141 ggaacgtacc ggatctcggg gaccaagatc ttcatctctg ccggcgagca cgacatggcg
     1201 gagaacatcg tccacctggt tcttgcccgc attccggacg cgcctccggg aaccaagggc
     1261 atatccatgt tcgtcgtgcc caagttcgat ctcgacggca cgcgcaatgg cgtgtcgtgt
     1321 ggctccatcg aggagaagat gggcatccac ggcaacgcca cctgcgttct caacttcgac
     1381 ggcgccaggg gctatctggt gggcgagccg aaccacggca tgcgttacat gttcacgatg
     1441 atgaacggcg cgcggctggt ggtgggtctg cagggcgcca gccaggccca cgcggcctgc
     1501 ctgctgtcgt tggaatacgc ccaggatcgc ctgcagatgc gctccctcac ggggcccaag
     1561 gcgccggagc ggccggcgga tcccatcatc gtgcacccgg acgtgcggcg aatgctgctc
     1621 ttccagcgcg ccatcgcgga agggggacgg gcgctcgtct actacgccgc acagatcatc
     1681 gatcgtttcc gggacgggtc cccggatgag cgggcttcgg ccggcgagct gctcgatttt
     1741 ctgacccccg tggtcaaggg tatcctgacg gagcttggct tcgagtcggt caatcaagcc
     1801 gtgcagattt acggtggcca cggcttcatc cgggagaccg gtgtcgagca gtacgtgcgc
     1861 gacaccagaa tcaccaccat ctacgagggc accacgcaga tacgggccct cgatctcgtg
     1921 ggtcggaaaa ttctgcagaa ccagggcaag gggctcgtca gcttcatcgg cgagatgcag
     1981 gccgttgccg cctccctgga ggatccgctg ccccgtctgg ccgctcctct caaagcgctt
     2041 gccgacgagt ggggcgagct cacgctgcag ctcagcgcga cggccatgca ggatctggat
     2101 gaggtaggtg ctgcggccgt ggactacctc tactacagcg ggtacgcggc gctcgctttc
     2161 tgctgggctc tcatggcgcg cagcgcggac gaggcgattc gagcggggtc gacggatccc
     2221 tttcatgcag gcaagattgc ggtcgccgac tttttcttca gtcgcgttct gccgcgcgcg
     2281 aaggcacacc aggctgccat cgaagcgggc aaagggactc tcatggacgt cgacgaaggt
     2341 gcgctggtcg cgctctgaac accgtgcgct tgaagtcatg attgccttgg acggcagggg
     2401 gaacccatgc tcgagtatca ggccccgctg agagacttcg actttcttct gttcgatgtg
     2461 tttcgggcgg gggagacctg gggccgtatt cccgcatttg ctgagctatc cgaagaactc
     2521 gttcgcgccg tgctggccga ggggggaagg gtcgccagag aagtaatggc accgctcaac
     2581 caaaccggag acgagcaagg ctgtcgttgg gacgacggcg tcgtcaccgc gcccgcgggc
     2641 ttcaaagccg catacgacga catggccggc ggcggctggc tggggatctc gggcaatccg
     2701 gagttcgagg gtcagggcat gccgaagatg ctcggatgtc tggtcgagga gatgttctgg
     2761 tccgcaaaca cgagcctgtt cctgtacggc acgctttcgg tgggggcctc gatctgcatc
     2821 gacacccatg gctcggctgc gcagaaatcg ctctacctgc caaagctcta cagcggccgc
     2881 tggagcggcg ccatggacct caccgagccc cacgcaggct cggatctcgg catgatgcgc
     2941 acgcgggcgg agtcggcggg cgacggcacc taccggatca cgggcacgaa gatcttcatc
     3001 accagcggcg agcacgacct gtccgagaac atcgttcatc tggtgctggc aaaggttccg
     3061 ggggcgccgg cgggcagccg gggcatatcg ttgttcatcg tgccgaagtt cctgcccgac
     3121 gccgatggcg agcccggcga gcgcaacgcg ctggccagcg gatccatcga gcacaagatg
     3181 ggcattcgtg gcagcgccac gtcggtcatg aacttcgacg gcgcggtcgg ctttctcgtc
     3241 ggcgaggaga accagggcct cgccgcgatg ttcaccatga tgaattacga gcggctctcg
     3301 gtgggcctgc agggcctggg cgcaggggag ctcgcctacc agcaggcggc gcgttatgcc
     3361 accgagcgaa ttcagggtcg ctccccggag ggaccgcaaa aacccgaggc tgccgcggac
     3421 ccgctgatcg ttcatccgga cgtgcgccgc atgctgctca cgatccgatg tctcactgaa
     3481 gcaggtcggg cgttcgggct cttcgccggc atgcagctcg atctgggcaa gcacctgggt
     3541 gatccggacg ccctggccct tggcgacctg ctcaccccga ttgccaaagc gtttctgagc
     3601 gatcgcggcc tggattgcgc cgtcctggga caacaggtct tcggcgggca cggatacgtt
     3661 cgtgagtggg gaatggagca gatcgtccgg gatgcccgca tcgcgcagat ctacgagggc
     3721 accaacggca ttcaggccca ggacctgatc gggcggaaga ttctgcgcga cggtggtgcg
     3781 gtgctgcgcc ggctcgccac catcgtcgag gctgatgccc gcgactcggc gtaccgcgcg
     3841 gatctgctgg ctgccgtcgc gcggctggtg aaggtcacgg actctctggt gcagcgttcg
     3901 cgcgccgatg ccgctctgcc gggagcggtg gccgtcgact accttgatct cgccggttat
     3961 accctgtacg cctggctgtg ggcccgaatg gcggacgcgg cccccggtga cgactttggc
     4021 cgcagcaagc gggcctgtgc cgagttttac ttcaagcggc ttttgccgcg gacgctgggc
     4081 ctcgccgctg ccatcgaggc ggaatccgag gcggtcatgg cgctagccgc cgacgcgttc
     4141 tagccggccg tggcggcggg ctgctcgggc gcctcgcccg acgtcgggca ggggtgctcg
     4201 ggacgggtgg ggtcgcagac ggtcaccgtg cgcatgtagg ccgcccacac cgccatctcc
     4261 gcatcgctga cgggcggtcg cgtgctctcc ccggaggttg tccgtctggt cagcgcgatg
     4321 acgtagaaat tctcgtttcc ccggatggct ttgatcaggg tcacctggtc caggtccgga
     4381 gccgcgtcgt tgcgacagga gaagagccgg acggacgtgg cgtagttgtt ctcctggccg
     4441 gaatgaatgt tcagatccga tgactgtccg cacccggccg cctgatccct gcccagcgcc
     4501 gtcagaaatt cgatgggatc cggcagcgga tcccccgcca tggattccag gcgaagcatc
     4561 tgctcccagt tgtcggcgtc ggtgtcggcc gggacgtatt gggccactcg cagccccgga
     4621 tcctgggtgg cagaccatac cttccagccc tccggcggtc gggccatgag cagctccggg
     4681 gaagccggct cggcgccggt tcgggcctgt gcctgcgcgg tgtcctcggc gccggagccg
     4741 gtgtcgcatc cggcaagc
//
LOCUS       DBNHQR010000536         4645 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1004263, whole genome shotgun sequence.
ACCESSION   DBNHQR010000536 DBNHQR010000000
VERSION     DBNHQR010000536.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1004263"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..1327
                     /gene="ald"
                     /locus_tag="AC16VR_12205"
     CDS             206..1327
                     /gene="ald"
                     /locus_tag="AC16VR_12205"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011920710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000286 - L-alanine dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0042853 - L-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HIR6011963.1"
                     /translation="MRIGVPREIKADEYRVGMTPASVRETVEHGHEVFVQSLAGEAVG
                     LHDADYQEAGARVVQDAEAVFESAELIVKVKEPQPEEAALLRPDHILFTYLHLAPNPS
                     LTLALSESGCTAIAYETVTDTQGGLPLLTPMSAVAGRMAVQAGAHCLEIEQGGRGLLL
                     GGVPGTPPARVTVLGAGVVGSNAARVALGMEADVTILDTSLRKLAELDLHFGPALNTI
                     FSTADAVQEFVLDADLVVGAVLVPGSETPKLISEAMVTEMRKGSVLVDVAVDQGGCAA
                     TSRPTTHSNPTYTVDDVVHYCVANMPGAVARSSTFALNNATLPFVLAIADKGAVTAMR
                     DDAHLRHGLNVCAGRVTYAAVASSAGLPFAEAEDVLRNP"
     gene            complement(1324..1536)
                     /locus_tag="AC16VR_12210"
     CDS             complement(1324..1536)
                     /locus_tag="AC16VR_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6011964.1"
                     /translation="MGENDDVVAACGGCHQGPLALDGFGADDLASRLRRIRDGDTPHP
                     PLNLEADDDHALEDLARRILAAGSDG"
     gene            complement(1645..2073)
                     /locus_tag="AC16VR_12215"
     CDS             complement(1645..2073)
                     /locus_tag="AC16VR_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR6011965.1"
                     /translation="MSIEPQRVRDYMSRELLTVSPETDIMRAIHLLVEHDISGLPVVD
                     AQDRLCGILTERDCIRIAVQAGYFDEAGGRVADYMSPSVHTVTPDDSLMDLAELFVES
                     PFRRCPVVEDGRLVGLICRRDVLRALKGGAWFADAGEHPR"
     gene            complement(2095..3318)
                     /gene="nqrF"
                     /locus_tag="AC16VR_12220"
     CDS             complement(2095..3318)
                     /gene="nqrF"
                     /locus_tag="AC16VR_12220"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HIR6011966.1"
                     /translation="MLEIVVPAVALTTIVFLLSLAVLLVRRGLVPGEDVEILINDQRA
                     LRGDAGEKLLWALADHDVYLPAACGGRGSCGQCRIEVTRGGGAITPAEENHIARRDAA
                     RGVRLACMVTLGDDVAVHLPPELLEIRRWSSKVLANRHVTTYLKELTLELPPGLELNF
                     NAGAYVQLEAPAGIVRFDGFDIAAEYDAEWQRHGLRDLVVEIPETTTRAYSLASHPGE
                     SGAIKLVVRIALPPPDAPVGTPPGRVSSYAFGLKPGDEVWLAGPFGEFRARDTDREMV
                     LIGGGAGIAPLRAIVLDQLLNKRSGRKISFWYGARNERELCYHSELTELARQHENFAY
                     RVALSDPAPDSAWQGLTGLVHAVVYREYLEGHPSPEEAEYYLCGPPLMSSAVLAILED
                     LGVDRDSVFFDDFGS"
     gene            complement(3321..3929)
                     /gene="nqrE"
                     /locus_tag="AC16VR_12225"
     CDS             complement(3321..3929)
                     /gene="nqrE"
                     /locus_tag="AC16VR_12225"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HIR6011967.1"
                     /translation="MEHLLNIVVRAIFVENLALVFLLGMCTYIAVSKRVETAIGLGVA
                     VVVVQLITVPVNNLLYEYVLTPGALAWMGLGTLDLSFLKLITFIGVIAALVQMLELVI
                     ARFAPQLQQHLGIYLPLLTVNCAILGASLLMVQRSYDFAESVAYGLGVGIGWAMAIVL
                     FAAIRERLKYSDVPKGLEGLGLAFIVTGFLAFGFIGFTGVEF"
     gene            complement(3929..4579)
                     /locus_tag="AC16VR_12230"
     CDS             complement(3929..4579)
                     /locus_tag="AC16VR_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HIR6011968.1"
                     /translation="MLRHLTHPLVSENPITVQILGLCSALAVSSTLKPAFIMSVSVVA
                     VLVFSNVSVSLLRNVFPRSIRLILEVTLIASAVIVVDEVLKAFAPETADILTVFVGLI
                     VTNCIILGRAEAFSMHHRVAPSLADALGNGLGYALVLLAVATARELFGHGTLLDVQIL
                     PLSEAEGGWYRPNQMMRFAPSAFFLIGLIIWGVRSVRAEQVEAGAYPPASPSYREG"
BASE COUNT          888 a         1528 c         1510 g          719 t
ORIGIN      
        1 gacgcgttct tgccgcccac cagggcaaga tcggccgagg cgatttccga gaaccatcga
       61 acataaccgg gcgctgccgc catgagccct ccctcgcatt gccgtttctg ctccctcagg
      121 ccccgacgtt atctaggggt aattccgtaa tcaatggcct ttcgcggacg tgtacaaacg
      181 caccacgcac aaaaaggtga aagacatgcg aatcggtgtg cccagagaga tcaaggccga
      241 cgagtaccgg gtgggcatga cgccggcgag cgtccgggaa accgtcgagc acggacacga
      301 ggtattcgtg cagtccctgg caggcgaagc cgttggcctg cacgacgccg actatcagga
      361 ggcgggcgcc agggtggtcc aggacgccga ggcggtgttc gaatcagccg agctcatcgt
      421 caaggtgaag gagccccagc cggaggaagc cgcgctgctg cgcccggacc acatcctctt
      481 cacctacctg catctggctc ccaacccttc gctcaccctg gcgctgagcg aatccggatg
      541 cacggcgatt gcttacgaga cggtcaccga cacccagggc ggcttgcccc tgctgacgcc
      601 catgagcgcc gtggccggac gcatggccgt tcaggctggc gcccactgtc tggaaatcga
      661 gcagggcggc cgcggtctcc tgcttggcgg cgtgcccggc acgccgccgg ccagggtcac
      721 cgtgctgggc gccggcgtcg tcggcagcaa cgcggcgcgg gtggccctgg gcatggaggc
      781 cgacgtgacc attctcgaca cctcgctgcg caagctcgcc gagctcgacc tgcatttcgg
      841 acccgcgctc aacaccatct tctcgacagc ggacgccgtg caggagttcg tgctggatgc
      901 ggacctggtg gtaggggccg tgctggtgcc cggcagcgag acgccgaagc tgatctcgga
      961 agccatggtg accgagatgc gcaagggctc ggtgctggtg gacgtcgccg tcgaccaggg
     1021 cggttgcgcg gccacctctc ggcccaccac gcattcgaac cctacctaca ccgtcgacga
     1081 cgtcgttcac tactgcgtcg caaacatgcc cggcgcggtc gcccgctcgt ccaccttcgc
     1141 gctcaacaac gccaccctgc cattcgtgct ggccatcgct gacaaaggcg cggtgaccgc
     1201 catgcgcgat gacgctcacc tgcgtcacgg gctgaacgtt tgcgcaggga gggtcacgta
     1261 cgcggcggtc gccagctcgg cgggccttcc tttcgccgag gcggaggacg ttctgcggaa
     1321 tccttagccg tcgctgccgg ctgccaggat ccgccgcgcg aggtcctcga gggcgtgatc
     1381 gtcgtctgct tcgaggttca gggggggatg gggcgtgtcg ccgtcacgga tacgcctgag
     1441 tcggctcgcg aggtcgtcgg ctccgaagcc gtcaagggca agcgggccct gatgacagcc
     1501 accgcaggct gccacgacgt cgtcgttctc tcccatcgcc gtgcctgcct ctaggcaaaa
     1561 ggaaacactc agcatgccgg cggacaataa cgcgcgtctt cgattcaacc tcgacccctt
     1621 cccggaaact gcggagctgc aatcctaccg tgggtgctca ccggcgtcag caaaccaggc
     1681 gccgccttta agggcgcgca gaacgtcgcg ccggcagatg agccccacca gccggccatc
     1741 ctccaccacc gggcagcgtc gaaaaggcga ctccacaaaa agctcggcga ggtccatcag
     1801 gctgtcgtcg ggcgtgaccg tgtggaccga aggggacatg tagtccgcca cccggccgcc
     1861 cgcctcgtcg aaatacccgg cctgaaccgc tatgcggatg caatcccgct ccgtcagtat
     1921 cccgcagagg cggtcctgcg cgtccaccac cggcagtccc gaaatgtcgt gctcgaccag
     1981 cagatgaatg gcccgcatga tgtcggtctc gggcgagacg gtcagaagct cccgcgacat
     2041 gtagtccctt acccgttgag gttcgatgct catgatcatg cctcctttgt ccgatcagga
     2101 tccgaagtcg tcgaagaaca cgctgtcgcg gtccacaccg aggtcttcga gaatggccag
     2161 gaccgcgctg ctcatcagcg gcggcccgca gaggtagtac tcggcttcct cgggagacgg
     2221 atgcccctcc aggtattccc tgtacacgac ggcgtgcacg aggccggtca gtccctgcca
     2281 ggcggagtcc ggcgctggat ccgacagcgc cacgcggtag gcaaaattct cgtgctgacg
     2341 ggcgagctcg gtcagctccg agtggtagca gagctcccgc tcgttgcggg cgccgtacca
     2401 gaaactgatc tttcgccccg accgcttgtt gagcagctgg tcgagcacga tggcgcgcag
     2461 cggcgcgatg cccgcgccgc cgccgatgag aaccatttcc cggtcggtat cccgcgcgcg
     2521 aaactcgccg aacgggcccg ccaaccagac ttcgtcaccg ggcttcaggc caaacgcgta
     2581 ggacgagacc cgcccgggcg gggtccccac cggggcatcc gggggcggca gggcgatgcg
     2641 aacgaccagc ttgatggcgc cggattcgcc ggggtggctt gcaagagaat acgcgcgggt
     2701 ggtcgtctcc ggaatttcca cgacgagatc ccgaaggccg tgccgttgcc attcggcgtc
     2761 gtactcggcg gcgatgtcga agccgtcgaa ccgcacgatg ccggccgggg cctcgagctg
     2821 cacgtaggcg ccggcgttga aattcaactc caggccaggc ggcagctcga gggtgagctc
     2881 cttcaagtag gtggtcacgt gacggtttgc cagaaccttc gagctccagc ggcggatctc
     2941 cagcaactcg ggaggcagat gcacggcgac gtcgtcgcca agggtgacca tgcaggcgag
     3001 gcgcacgccg cgagcggcat cacggcgcgc aatgtggttc tcttccgccg gcgtgatggc
     3061 gccaccgccc cgcgtcacct cgatgcgaca ttgcccgcag ctgccccggc caccgcaggc
     3121 cgccggaaga tagacatcgt ggtcggccag cgcccacagg agcttctcgc cggcgtcgcc
     3181 ccgcagagcc cgctgatcgt tgatcagaat ctccacgtcc tcgccgggca ccaggccgcg
     3241 gcggacgagg agcaccgcca gcgagagcag gaagacgatg gtcgtcagcg cgacggctgg
     3301 cacgacgatc tccagcacgt ctagaactcc actccggtga agccgatgaa gccgaaggcg
     3361 aggaacccgg tcacgatgaa ggccaggcca aggccttcga gccccttcgg cacatcgctg
     3421 tacttcaagc gctctcggat ggcggcaaag agaacgatgg ccatcgccca gccgatgccg
     3481 acgccgagcc cgtaagcgac gctttcggcg aagtcatagc tgcgctgcac catgagcagg
     3541 gaagcgccga gtatggcgca gttgacggtg agcagcggca ggtagatgcc caggtgctgc
     3601 tgcagctgcg gcgcgaaccg cgcgatgacg agctccagca tctggacaag agcggcgatg
     3661 acgccgatga aggtgatcag cttgaggaac gaaagatcca gggttcccag ccccatccag
     3721 gccagcgccc cgggagtgag gacgtattcg tagaggaggt tgttgacggg cacggtgatg
     3781 agctgcacga cgacgacggc gacgccgagc ccgatggcgg tctccacacg cttggagacc
     3841 gcgatgtacg tgcacatgcc gagcaggaac accaaagcca ggttctcgac gaagatggcg
     3901 cgcacaacga tgttcaggag atgctccatc agccctcccg gtagctcggg ctcgccggcg
     3961 ggtaagctcc ggcttcgacc tgttccgcgc gcacgctgcg aacgccccag atgatgagcc
     4021 cgatcagaaa aaaagcgctg ggcgcaaagc gcatcatctg gttggggcga taccaaccac
     4081 cttccgcctc gctcaggggc agtatctgaa cgtcgagcaa cgtcccgtgc ccgaacagct
     4141 cgcgagccgt ggcgacggcc agcaacacca gcgcgtagcc cagaccattc ccaagggcgt
     4201 cggcaagact gggggccacc cggtggtgca tggaaaacgc ttcggcccgt ccgagaatga
     4261 tgcagttggt gacaatcagg ccaacgaaca cggtcagtat gtcagccgtc tccggagcga
     4321 aggccttgag cacctcgtcc accacgatca ccgccgaggc gatgagcgtc acttccagga
     4381 tcagacgaat gctgcgggga aagacgtttc tcaacaggct gacggacaca ttcgagaaaa
     4441 ccagcaccgc gacaacggaa acgctcatga tgaaagccgg cttgagggtg ctggacaccg
     4501 caagggcgga gcagagaccg aggatctgca cggtgatcgg gttctcgctg acgagcggat
     4561 gggtgagatg cctcaacatg aatggtgtcc acgcttcata ttcgtgctcc aggcgccgcg
     4621 atccgggaaa gccagggcga gtaga
//
LOCUS       DBNHQR010000537         4319 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_876674, whole genome shotgun sequence.
ACCESSION   DBNHQR010000537 DBNHQR010000000
VERSION     DBNHQR010000537.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_876674"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="AC16VR_12235"
     CDS             complement(<1..180)
                     /locus_tag="AC16VR_12235"
                     /inference="COORDINATES: protein motif:HMM:NF014998.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase
                     domain-containing protein"
                     /protein_id="HIR6011969.1"
                     /translation="MTLSDRLLDLGFLEAETPRTPMHFSALMFFDTPSDPAPFVSGLR
                     EAWTGAVPVAPFDLVP"
     gene            complement(190..669)
                     /locus_tag="AC16VR_12240"
     CDS             complement(190..669)
                     /locus_tag="AC16VR_12240"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013062.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HIR6011970.1"
                     /translation="MTLAAEERAELEEVYARYADLLDDGPLADWPALFHEECLYLLIP
                     RENHDRGLPLAIMRCESRGMLEDRVRAVQETIMHEPRYLRHHITNVRGARTDDGGYSV
                     IANYSVIEVLQDELPRILSVGRYLDVLTRSPTGALMFREKRVVFDSVMVPNTIVYPL"
     gene            complement(666..1898)
                     /locus_tag="AC16VR_12245"
     CDS             complement(666..1898)
                     /locus_tag="AC16VR_12245"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6011971.1"
                     /translation="MDVDGATWSWPDGGVTEVPYAIYTDPGIFAREMERIFSGPSWAY
                     VGLEAEIPQPGDYLVTRIGVRSVVIARDEEGAIHGFANRCAHRGVKVCRQRHGNARFF
                     VCPYHQWSYDARGRLRGVPFINGVRQQGGMPEGFAREDHNLPPLDIATRGGVIFASFS
                     ATAPRLEDYLGDTNLSYFDRVFDGRQLKVLGYSRQRIPGNWKLMFENIKDPYHASLLH
                     VFLVTFGLFRADQPAQVRMDPSGAHGLLISRRGEQGATADTGEMSSMKEDFSLADARL
                     LDPVKEFPDDATVVMQTIWPNLIVQQQSNTLALRQIQPLDPGRFELHWTFFGYGDDDD
                     AMTLRRLRQANLMSSAGLVSADDSEVIKLAQDGVAQYPDDVGVMLMGGRGTDDEDHMV
                     TETAIRAFYAHYRRVMGL"
     gene            1998..3107
                     /locus_tag="AC16VR_12250"
     CDS             1998..3107
                     /locus_tag="AC16VR_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6011972.1"
                     /translation="MSDRAPLIVDRTGEPLTTSEPARSKPARDWPPITITREMIDEEI
                     ERLADLPQPANGRRQAMFVHPNAPDPGRGLTPGVQVTLDVLKPGEETVPVRHNSTQVS
                     FCIRGGGTAIVGDRPIRYAQYDAWNHPSYATYRHVNDSRELQARLTYSNAALLEMMRV
                     HLVEEDPQDEPVARSAQAPAATGAQTETFRIGEDGAALMPYETLINPPSVASDALHWP
                     WRTVKANLDRLEALGADYVGRRLYLLFNPRTERFNGTTPNFFATITIRPPGIVDKPHR
                     HVSSAINYYFNGSGYSRVEGKRYDWKAGDLMVSAPGWAVHNHASNEGDPVYELTIQDQ
                     PLNIYMESLLWQEDLAEPARILGSHEGFSTNRSTL"
     gene            3128..3748
                     /locus_tag="AC16VR_12255"
     CDS             3128..3748
                     /locus_tag="AC16VR_12255"
                     /inference="COORDINATES: protein motif:HMM:NF013756.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR6011973.1"
                     /translation="MFYDPREDPQPEPLGHNPINALVAPRPIGWIASLSADGAPNLAP
                     FSYFNAFSADPPVVAFAPNEKSGAGGPKDTLANVRSVPEFTASLVSEPLARRMNESSR
                     VLPHGVSEFIEAGLTPAASRLVAPPYVGEALAALECRVFDIIELPGRPNGRRSHLVIG
                     EVVGIHIDDGVIVDGRIDSLALAQLARLGYHDYTVVRELIEIPRPD"
     gene            3750..4202
                     /locus_tag="AC16VR_12260"
     CDS             3750..4202
                     /locus_tag="AC16VR_12260"
                     /inference="COORDINATES: protein motif:HMM:NF024854.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR6011974.1"
                     /translation="MAPSTSDPDRSYGYQSRATHRAFDRLLQQHLRQHGLTNGYWYVL
                     RVLWEEEGMSQRELARASNLTESSAVIMLDGMQRAGLVARRKDPADGRRQRVFLTAKA
                     KRLKPKLLPVAGELNALAASGIAAEDLATYLAVAHRMRANLVAALETG"
BASE COUNT          809 a         1464 c         1363 g          683 t
ORIGIN      
        1 gggaaccagg tcgaagggcg ccaccggaac cgcgcccgtc caagcctctc tcaacccgga
       61 aacgaagggc gccggatcgg atggcgtatc gaagaacatc agcgcgctga agtgcatggg
      121 cgtgcgaggc gtctccgcct ccaggaagcc gagatcgagg agccggtcgg agagggtcac
      181 cgcgccgacc tacagcgggt agacgatggt gttgggcacc atcaccgaat cgaataccac
      241 ccgcttctcc cggaacatca gcgcgcccgt tggggatctt gtcaaaacgt cgagataccg
      301 acccacgctc agaatgcgcg gcaactcatc ctgcagcacc tcgatgacgg agtagttggc
      361 aatcaccgag taaccaccgt cgtcggtgcg agcaccgcgg acgttggtga tgtgatgccg
      421 aaggtagcgc ggctcgtgca tgatggtttc ctgaaccgca cggacccgat cctccagcat
      481 cccacggctc tcgcagcgca tgatggcgag cggcaggcct cgatcgtgat tctctctggg
      541 tataagcagg tagaggcact cctcgtggaa gagtgcgggc cagtccgcca gcgggccgtc
      601 atccaggagg tcggcatagc gagcgtagac ctcctccagc tcggcgcgct cctcggcggc
      661 gagggtcaca gccccatcac ccgtcggtaa tgagcgtaga acgctctgat ggcggtttcc
      721 gtgaccatgt ggtcctcgtc gtcagtgcct cggccgccca tgagcatcac cccgacgtcg
      781 tcgggatact gggcgacgcc gtcctgagcc agcttgatca cctcgctgtc gtccgcggag
      841 accagaccgg ccgagctcat caggttcgcc tgcctcagac gccgcagcgt catcgcatcg
      901 tcgtcgtccc cgtagccgaa gaacgtccag tgcagctcga agcggccggg gtccagaggc
      961 tgtatctggc gcagcgccag ggtattggat tgctgctgca cgatcagatt cggccagatg
     1021 gtctgcatca ccaccgtggc gtcgtccgga aactccttga ccggatccag gagacgcgcg
     1081 tctgccaggg agaagtcctc cttcatgctg gacatctccc cggtgtccgc cgttgctccc
     1141 tgctctcccc gccgcgagat cagcagccca tgggcgccgc tagggtccat gcgcacctgg
     1201 gcaggctgat ccgcccggaa cagcccgaag gtcacgagaa agacatgcag cagcgacgca
     1261 tgatagggat ccttgatgtt ctcgaacatg agcttccagt tcccggggat gcgctgccgc
     1321 gagtagccga ggactttcag ctgccggccg tcgaacacac ggtcgaagta ggagaggttg
     1381 gtgtcaccga ggtaatcctc gagccgcggc gccgtcgcgg agaaggacgc gaagatgacg
     1441 ccgccacggg tggcgatgtc gaggggcggc aggttgtgat cctcacgggc gaacccctcg
     1501 ggcattcctc cctgctgccg gacgccgttg atgaagggaa cgccgcgcag gcggccccgg
     1561 gcatcgtagg accactgatg gtaggggcag acgaagaatc gcgcgttgcc gtgacgctgg
     1621 cggcaaacct tcactccccg gtgcgcgcag cgattggcga agccgtggat cgcaccttcc
     1681 tcgtccctgg cgatcaccac ggaccgaacg ccgattcgtg taacaagata gtccccgggc
     1741 tgggggatct ccgcctccag cccgacgtag gcccagctcg gacccgagaa tatgcgttcc
     1801 atctcccggg caaaaatccc agggtccgtg tagatggcgt agggcacctc ggtcacccca
     1861 ccatcgggcc acgaccacgt cgcgccatcg acatccatga gctaaccccg cagttgacgt
     1921 cccacatatt actttgatat caaagtattt caacgcgccg gcttcgccgg tccagcttcc
     1981 cgctcgagag gccaagaatg agcgaccgcg caccactgat agtcgatcgc accggagagc
     2041 cgctgaccac ctcggagccg gcccgatcaa agccggccag ggactggccg ccgatcacca
     2101 tcacccggga gatgatcgac gaggagatcg agcggctggc agatctgccg caaccggcca
     2161 acggccgacg ccaggcgatg ttcgtccatc ccaacgcgcc ggatccaggc cggggcctca
     2221 ccccgggcgt tcaggtgacc ctggacgttc tgaaacccgg cgaggaaacg gttcccgtcc
     2281 gccacaattc cacccaggtg agcttctgca tccggggcgg cggcacggcc atcgtcggcg
     2341 accggcccat ccggtacgcg caatacgacg cctggaatca cccctcctac gccacctacc
     2401 ggcacgtcaa cgacagccgc gagctccagg caaggctgac ctactctaac gccgctctgc
     2461 tggagatgat gcgcgtgcat ctggtggagg aggacccgca ggacgagccg gtggcaagga
     2521 gcgcccaggc gcccgcggcc accggggcgc aaacggagac cttccgcatc ggtgaggacg
     2581 gcgcggcgct gatgccctac gagaccctca tcaacccgcc gtcggtagcc tccgacgcgc
     2641 tgcactggcc ctggcggacg gtgaaagcaa atctggacag gctggaagcg ctgggagccg
     2701 actacgtggg acgccgcctc tacctgctgt tcaatccccg aaccgagagg ttcaacggca
     2761 ccacgccgaa cttctttgcc accatcacga ttcgaccgcc gggaatcgtg gacaaacccc
     2821 atcgccacgt gtcctccgcc atcaactatt acttcaacgg ttcgggttac agccgcgtcg
     2881 agggcaaacg ctacgactgg aaggcaggtg atctcatggt gtccgcgccg ggctgggccg
     2941 tgcacaacca cgcctccaac gaaggcgacc cggtgtacga gctgaccatt caggatcagc
     3001 cgctgaacat ctacatggag tcgctgctgt ggcaggagga cctggccgag cctgcccgaa
     3061 tactcggatc tcacgaggga ttctccacca accgttcgac gctctgatcg ctgctgacgc
     3121 ccgaaacatg ttttacgatc cgcgagaaga tccccagccc gagccgctgg gccacaatcc
     3181 gatcaacgcc ctcgtcgcgc caaggcccat cgggtggatc gcctccctct ccgccgacgg
     3241 cgcgccgaat ctggcaccgt tctcctactt caacgcgttc tcggcagacc cgcccgtcgt
     3301 ggccttcgcc cccaacgaga agtccggcgc ggggggtccc aaggatacgc tggccaacgt
     3361 caggagcgtg cccgagttca ccgcgagcct cgtctccgaa cctctggccc ggcggatgaa
     3421 cgaatcgtcc cgcgtgctgc cccacggcgt cagcgagttc atcgaagccg gattgacccc
     3481 ggcggcgtcg cgcctggtgg ccccgccgta cgtgggcgaa gcgctggccg cgctggaatg
     3541 ccgcgtgttc gacatcatcg agctgccggg ccgcccgaac ggacgccgca gccacctggt
     3601 catcggcgag gtggttggga tccacatcga cgacggggtg atcgtcgacg ggcgcatcga
     3661 ctctctcgcc ctcgctcagc tcgcccgcct gggttaccac gactacaccg tggtgcggga
     3721 gctgatcgaa attccgaggc ccgactgaga tggcgccgtc taccagcgac ccggatcgca
     3781 gctacggtta ccagtcgcgg gccacccacc gcgccttcga ccggctgctg cagcagcacc
     3841 tgcgccagca cggtctcacc aacggctact ggtacgtgct gcgcgtgttg tgggaggagg
     3901 agggcatgtc acagcgggag ctggctcgag cgtccaacct gaccgagtcc tccgcggtga
     3961 tcatgctgga cggcatgcag cgcgcgggcc tggtggcgcg cagaaaggat cctgcggatg
     4021 gtcggcgcca gcgcgtcttc ctgacggcaa aggcgaaacg actgaaaccg aagctgctgc
     4081 cggtagcggg cgagctcaac gcccttgcgg cgagcggcat cgccgccgag gacctggcga
     4141 catacctggc cgtggcccac cggatgcggg cgaatctggt cgccgccctc gaaacgggct
     4201 gatcgagcga cgggccctag accggagcgt tggcgggcag tccgagcagc aggcgtccat
     4261 agagctcacc gcccacgtcc atgtcgaaca ccgtgtggca ggacagcgtg ttcaggtca
//
LOCUS       DBNHQR010000538         4383 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1321782, whole genome shotgun sequence.
ACCESSION   DBNHQR010000538 DBNHQR010000000
VERSION     DBNHQR010000538.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4383)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1321782"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..1460)
                     /locus_tag="AC16VR_12265"
     CDS             complement(120..1460)
                     /locus_tag="AC16VR_12265"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6011975.1"
                     /translation="MQRLKNSTLFYYSLTDLPIAMSMFPVAVFIPRFYASDVGVPLAV
                     VGTIILVVRIFDVVTDPLMGYLSDQTRTRFGRRKPWIFAATPIMMTSIYMLFMPPEDA
                     GWLHMLTWSMLLSIAITMMLIPYYAWGAELSPDYNERSRITGGRAMAGVFGSLSAQLI
                     PAGALLFFGLGGSDVVLTLVGTTMLVLMPICVLLTLLNTPEPEVRHKSTVPVGKGLKL
                     MWANGPFKQLVLAFMIGSIGLNITTPLYIFFIADVLGAEDQAIYMLTFFFLVSFGSVP
                     FWVKMARRIGKHRAYLTSFFLLAFAHPFYLLLGEGDFWWMLPVTFATGFASGGFSQTL
                     PNSMKADVIDLDTLESGENRAALFFSAWSFAQKATASVGSSIALFGLALFGFDAAPEA
                     VNGPDELFGLRFLFSTFPSLFFLTGAAIVWSYPITEKRHEEIRRGIEARLQAGR"
     gene            1646..2059
                     /locus_tag="AC16VR_12270"
     CDS             1646..2059
                     /locus_tag="AC16VR_12270"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6011976.1"
                     /translation="METAVKLAIHDLLGRSAYALDERHVDMLEGTFTEDAAFEIDIQG
                     VDGTMSFAGREAIMGLMTDSMAEQTDQRRHVVTNVFFEEVGEERARLISNITITSVEH
                     GQIRLVTSGLYRDEVVLHNGDWRIARRRIELDMPY"
     gene            2075..2854
                     /locus_tag="AC16VR_12275"
     CDS             2075..2854
                     /locus_tag="AC16VR_12275"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6011977.1"
                     /translation="MNLKGQVAVISGAAVGIGRGIAVALAEQGAAVACLDIDGVGNQE
                     TAGLAEEAGGRGLALDCDVADRAQVRRAMSEALGALGRIDVLVNNAAIYVDTSLTVGS
                     YDAQTRAYERSIAICALGSYYCARAAVPAMRESGGGNIVNIITEHIKEGHLMTGPGAT
                     GYDGAKWVMWRQTESWAVELAEHNIRVNALCMGATDTPMLRAVSVPIAEAGMRPEDVG
                     QAVLNVLAHGRRGPTGQSYVFGTTGTSREQSLGEIAALAPR"
     gene            2872..4191
                     /locus_tag="AC16VR_12280"
     CDS             2872..4191
                     /locus_tag="AC16VR_12280"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6011978.1"
                     /translation="MRDRFSQAYLWYVVAVLTIASTFSIIDRQILNVMIGPVKRDLGG
                     ISDFQVSLIMGFAFTFLYSVLSYPAGWVADRYNRKNLMAVGIAAWSAMTMLCGMVSQY
                     WHLFATRMGVGVGEATLQPAATSSLSDYFPPHMLPLAMGIVASAPFIGQGLANIAGGP
                     LIDYLEATPNYVLPLFGEVFSWQMVFLLVGAPGLLVALAMWGLREPSRHGKQTEEAGV
                     PLREVWAFVKDRGAFFFLVFTAYLCLATQGWSLFSWLVEFYVRNHEWTRTEIGLTYGF
                     IAMFVGMAGSIIGGAWAGRLIRRDSPDGTIRVVMYGSIVLLPVGAAITIVPDPWLGVA
                     LLVPATFCMAMPPGLIMATLQAIAPNELRGQMVAFYLIAVNFLAYTFAPSLPAVLSDF
                     VFGSEMALGKSISLLAVVNYTISSLCLALGLKHYRRALDRARAWASA"
     gene            complement(4241..4366)
                     /locus_tag="AC16VR_12285"
     CDS             complement(4241..4366)
                     /locus_tag="AC16VR_12285"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6011979.1"
                     /translation="MAVGQQQGFHSRGYRDAILSGQESRVRRFHEVLNDYLEGRR"
BASE COUNT          783 a         1365 c         1461 g          774 t
ORIGIN      
        1 gcgctacgtg gtgtccaccg aggatctcga gcggatgttc agcgagctgg atgagccaga
       61 cgcgctcgag aaagcgcctg ctgattccga gcaggacccc aaggcagcct gaacccgcgt
      121 caccggcccg cctgcagcct ggcttcgatg ccccgtctga tctcctcgtg acgcttttcc
      181 gtgatggggt agctccagac gatggctgcg cccgtcagga agaacaggga aggaaacgtc
      241 gagaacagaa aacgcagccc gaacagctcg tcgggcccgt tgaccgcctc gggcgcggca
      301 tcgaatccga acagggcgag cccgaacaac gcaatcgagc tgcccaccga agcggtcgcc
      361 ttctgagcga aggaccaggc ggagaagaac agggccgccc ggttctctcc gctctccagg
      421 gtgtcgaggt cgatgacgtc ggctttcatg gagttgggaa gggtctgcga gaagccgccg
      481 ctggcgaagc cggttgcgaa ggtgaccggg agcatccacc agaagtcgcc ctcgcccagc
      541 agcagataga aggggtgggc gaaggccagg agaaagaagg acgtcaggta agccctgtgc
      601 ttgccgatgc ggcgcgccat cttcacccag aacggcacgg agccgaagct gacgagaaaa
      661 aagaaggtga gcatgtagat cgcctgatcc tcggcaccga ggacgtcggc gatgaagaag
      721 atgtagagcg gcgtagtgat gttcaggccg atggagccga tcatgaaggc gaggaccagc
      781 tgtttgaaag ggccgtttgc ccacatcagc ttgagcccct tgcccaccgg taccgttgac
      841 ttgtggcgca cctcgggctc cggggtattg agcagggtga ggagcacgca aatgggcatc
      901 agcacgagca tggtcgtgcc caccagcgtc agtaccacgt cgctgccgcc caggccgaag
      961 aagagcaggg cgcccgcggg gatcagctgc gcgctcaggc tgccgaacac gccggccatg
     1021 gcccgtccgc ccgttatcct cgagcgctcg ttgtagtctg gtgagagctc cgcgccccac
     1081 gcgtagtagg ggatcagcat catcgtgatc gcgatggaca gcagcatgct ccaggtgagc
     1141 atgtgcagcc agcccgcgtc ttcagggggc atgaacagca tgtagatcga ggtcatcatg
     1201 atgggcgtcg cagcgaagat ccagggcttg cgccggccga accgggtgcg ggtttgatcg
     1261 ctgaggtagc ccatcagagg gtcggtgacc acgtcgaaga tccgtaccac caggatgatc
     1321 gtgccgacca cggccagggg aacgccgacg tcgctggcgt aaaaacgcgg gatgaacacg
     1381 gccacgggga acatcgacat ggcaatgggc aggtcggtaa ggctgtagta aaacagcgtg
     1441 gagtttttca gccgctgcac gtggatttcc ttccggggcg gcctattgga cttggcggcg
     1501 catttgcgtg tcaaggaagc tcgcaacggt ggcgcccggg ccgatcatcg cctcaacatt
     1561 cctggcgctg ctgcggctgg caatcctgca aggaatgcca taacgtcggg gctgcatcga
     1621 cggccggatc cgctgggagg ggaagatgga aaccgcagtc aagctcgcca ttcacgatct
     1681 gttggggcgc tccgcttacg ccctggacga acgtcacgtg gatatgctgg aaggaacgtt
     1741 tacggaagac gctgccttcg agatcgatat ccagggcgtc gacggcacga tgtccttcgc
     1801 gggtcgcgag gccatcatgg ggctgatgac cgattccatg gcggagcaga ccgatcagcg
     1861 acgccacgtg gtcacgaacg tgtttttcga ggaggtggga gaggagcgcg ccaggctgat
     1921 ctcgaacatc accatcactt cggtggagca cggccagatc cgtctggtca cctccggcct
     1981 gtatcgggac gaggtggtgc tgcacaacgg tgattggcgc atcgcccggc ggcggatcga
     2041 gctggacatg ccttactgat cccaaggagg ctgcgtgaat ctcaaaggtc aggttgccgt
     2101 catcagtggt gcggccgtgg gaatcgggcg cggcatcgcc gtggctctgg cggaacaggg
     2161 cgcggcggtc gcttgcctgg acatcgacgg cgtgggaaac caggagacgg ccgggctcgc
     2221 ggaggaggcg ggtgggcgcg ggctcgcgct cgactgtgat gttgccgatc gagcccaggt
     2281 gcggcgtgcc atgagcgagg cgctgggcgc gctgggaaga atcgacgtgc tggtgaacaa
     2341 cgccgccatc tacgtggaca cctctctcac ggtgggcagc tacgatgccc agacccgcgc
     2401 gtacgaacga tccatcgcca tctgcgccct gggcagctac tactgcgccc gggccgccgt
     2461 gcccgccatg cgcgagtccg gcggcggcaa catcgtcaac atcatcaccg agcacatcaa
     2521 ggaagggcac ctcatgaccg gaccgggcgc cacaggttac gacggtgcga agtgggtaat
     2581 gtggcggcag accgaatcct gggccgtgga gctcgccgag cacaacatac gggtcaacgc
     2641 gctctgcatg ggcgctaccg acacgccgat gctgcgggcg gtttcggtgc ccatcgccga
     2701 ggccggcatg cggccggagg acgtgggcca ggcggttctg aacgtgcttg cccacggtcg
     2761 gcgcggaccg acggggcaat cctacgtctt cggcaccacg ggcacgtccc gggagcagag
     2821 tctcggcgag atcgccgctc tggctccgcg ctgagcccgc cccgggcacc catgcgggac
     2881 aggttcagcc aggcatacct ttggtacgtg gtggcggtgc tgaccatcgc ctccaccttc
     2941 tccatcatcg atcgtcagat cctgaacgtg atgatcggac ccgtgaagcg cgacctgggc
     3001 ggcatctcgg atttccaggt ctcgctcatc atgggcttcg ccttcacctt cctctacagc
     3061 gtgctttcct atccggccgg ctgggttgcc gaccgctaca accggaagaa cctgatggcc
     3121 gtgggcattg ccgcctggag cgcgatgacc atgctttgcg gcatggtgag ccagtactgg
     3181 cacctcttcg ccacgcgcat gggggtcggg gtcggcgagg cgactctgca accggcggcc
     3241 acgtcctcgc tttccgacta ctttccacct cacatgctgc cgctcgccat gggcatcgtg
     3301 gcctcggcgc cattcatcgg gcagggcctg gccaacatcg ccggcggtcc tcttatcgac
     3361 tacctggagg caacacccaa ctacgtgctg ccgctctttg gtgaggtatt ttcctggcag
     3421 atggtgttcc tcctggttgg cgcgcccggg ctgctggtgg cgctcgccat gtgggggctg
     3481 cgcgagccgt cccgacacgg caagcagact gaagaggccg gcgtgccgct ccgcgaggtc
     3541 tgggccttcg tcaaggatcg gggggctttc ttcttcctgg tcttcaccgc ctacctgtgc
     3601 ctggctaccc agggctggtc acttttctcc tggctcgtgg agttctacgt gcgcaaccac
     3661 gagtggacgc gcacggagat cggactgacc tacgggttca tcgccatgtt cgtcggcatg
     3721 gcgggcagca tcatcggcgg ggcctgggcg gggcggctga tccgccgcga ctcgccggac
     3781 ggcacgatcc gggtcgtcat gtatggatcc atcgtgctcc tgccagtcgg ggccgccatc
     3841 accatcgtcc ccgacccctg gctgggcgtg gctctgctgg tgcccgccac gttctgcatg
     3901 gccatgccgc cgggcctcat catggcgacc ctgcaggcca tcgctcccaa cgagctgcgc
     3961 ggccagatgg tggccttcta tctcatcgcg gtgaactttc tcgcgtacac gttcgcgccg
     4021 tcattgccgg ccgtgctgag cgatttcgtg ttcggcagcg agatggcgct gggtaagtcc
     4081 atctcgctgc ttgccgtcgt gaactacacg atctccagcc tctgcctggc cctgggtctg
     4141 aagcactatc gacgcgcttt agaccgcgcg cgggcgtggg cgagcgccta gcgctccgac
     4201 gacatctcaa gcaggccgat ttccacgcag ctggcggatg ctaacggcga ccttccagat
     4261 agtcgtttaa aacctcgtgg aagcgccgca cccggctctc ctgaccgctt aagatcgcgt
     4321 cgcgatagcc gcgggaatga aacccctgct gctggccgac cgccaccccg agatcctgga
     4381 tca
//
LOCUS       DBNHQR010000539         2591 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_54638, whole genome shotgun sequence.
ACCESSION   DBNHQR010000539 DBNHQR010000000
VERSION     DBNHQR010000539.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_54638"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1269)
                     /locus_tag="AC16VR_12290"
     CDS             complement(<1..1269)
                     /locus_tag="AC16VR_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010324201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR6011980.1"
                     /translation="MSQLLHENLAKLDLHLARFRNRTLGHLIDGEEAEGVSGETFTNT
                     TPVDDSPVNEVARGAEEDVTRATRAAVRAFGEWRDMPGARRRGLLHGIADAIEARAEE
                     IALVESYDTGQPLRFMSSAALRGAENFRFYADRAPEARNGLSTPADQHVNYSVRQPIG
                     PVGVITPWNTPFMLSTWKIAPALAAGCTVVHKPAEWSPLSASILAEICRDAGLPAGVL
                     NTVHGLGEEAGVALTEQPDIRAIAFVGESQTGRAIMSQGASTLKRVHFELGGKNPVIV
                     FDDADLERALDACTFMIYSLNGERCTSSSRLLVQATIYDEFCARVAERAGQIRVGHPL
                     DPATEVGPLIHPRHLEKVLGYCALAQTEGATVAVGGGPPGDAAAGNYVAPTLFTHARN
                     DMRIAQEEIFGPVLTAIPFDDEAEAVELANQ"
     gene            complement(1266..1697)
                     /gene="hpaR"
                     /locus_tag="AC16VR_12295"
     CDS             complement(1266..1697)
                     /gene="hpaR"
                     /locus_tag="AC16VR_12295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HIR6011981.1"
                     /translation="MLLLRARETSMAYFRPSLQERGLTEQQWRVLRALQESGELSASG
                     LSHACVILAPSMTRILRRLAEEGLVSSKRSRRDGRELTVRLTRAGERLIDEIAPISER
                     QYALIRERLQPEKLEQLHSLLHDFVELDGEHLKGAASGETQ"
     gene            complement(1812..>2591)
                     /locus_tag="AC16VR_12300"
     CDS             complement(1812..>2591)
                     /locus_tag="AC16VR_12300"
                     /inference="COORDINATES: protein motif:HMM:NF015214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase C-terminal
                     domain-containing protein"
                     /protein_id="HIR6011982.1"
                     /translation="PIDTRGLRLICRDTYDAGKSHFDAPLASRFEEMDAVVIFDNVLV
                     PWERVFMYQAPLLCNRAFAETNAVVHMMHQVACGKLAKAEFIVGLLCAMAKASGKDKD
                     ANVKGQIAEAMWIVETVRAFLFSAEQQAEPDRWGLWVPKRLPLDTSRNLFPRLYPKLV
                     ETVHMLGSSSLMATPCEADFENAIAGDVEQYFQLTNLESRDRVALFRLAHDVAVSGFG
                     NRQVLYERFFFGPQPVMASIYYDLYDKDSVMDRVTEMLNGC"
BASE COUNT          454 a          856 c          810 g          471 t
ORIGIN      
        1 ctggttggca agctcaacgg cctcggcttc gtcgtcgaaa gggatcgccg tcagcacggg
       61 accgaatatc tcctcctggg cgatacgcat gtcgttgcgc gcgtgggtaa agagcgtggg
      121 cgccacgtag ttcccggcgg ccgcgtcccc cggagggccg cccccgaccg ccacggtagc
      181 cccttcggtc tgcgccagcg cgcagtaccc gagcaccttt tcgaggtggc gcggatgaat
      241 cagcggtccc acctcggtgg cgggatccag cgggtggccc acacggatct gcccggctcg
      301 ctcagccacc cgggcgcaga actcatcgta gatcgtcgcc tgcacgagca gtcggctcga
      361 cgaggtgcac cgctcgccgt tgaggctgta gatcatgaac gtgcaggcat ccagcgcgcg
      421 ttcgagatcg gcgtcgtcga acacgatgac cggattcttc ccgccgagct cgaagtgcac
      481 gcgcttgagc gtgctcgcgc cctggctcat gatcgcgcgg cccgtttggg actcgcccac
      541 gaaagcgatg gctcggatgt ccggctgctc cgtgagcgcc acgccagcct cctcgcctag
      601 cccatgaacc gtgttgagca cgccggcagg cagcccggca tctcgacaga tctccgcgag
      661 tatgctggcc gagagggggc tccactctgc cggcttgtgc accaccgtgc agccggcggc
      721 cagagccgga gcgatcttcc aggtggagag catgaacggc gtgttccaag gggtgatgac
      781 gccgaccggc ccgatcggtt ggcggacact gtagttcaca tgctgatcgg cgggagtgga
      841 gaggccgttg cgggcttccg gcgctcggtc ggcataaaac cggaagtttt cggctccgcg
      901 cagcgccgcg ctgctcatga aacgcagcgg ctggccggtg tcgtacgact ccaccagggc
      961 aatctcctca gcccgcgcct cgatggcatc ggcgatcccg tggagcaggc cgcgccggcg
     1021 ggcacccggc atgtcacgcc actcgccaaa cgcccttacg gctgcccgcg tcgctctggt
     1081 tacgtcttcc tcggcacccc gggccacctc gttcaccgga gagtcgtcca ccggggtagt
     1141 gttcgtaaac gtctcgcccg agacgccttc ggcttcctca ccgtcgatga ggtgccccag
     1201 cgttcggttg cggaaacgcg ccaggtgcag gtcgagcttt gccaggtttt cgtgaagcag
     1261 ttggctcatt gggtctctcc cgatgcggcc cctttcaggt gctcgccatc cagttccacg
     1321 aagtcgtgca gcaggctgtg aagctgctcc agcttttccg gttgcagccg ttcgcggatg
     1381 agcgcgtact gacgctcgct gatgggcgcg atctcgtcga tcagacgctc gcccgcgcgg
     1441 gtcaacctca ccgtgagctc cctgccgtcg cggcgcgacc gcttcgacga aacgagcccc
     1501 tcctcggcaa gccgacgcag tatccgcgtc atgctgggcg ccagaatcac gcacgcgtgt
     1561 gaaagccccg acgccgacag ctcgccagat tcctgcaacg cccgaagcac acgccattgc
     1621 tgctcggtga gcccccgctc ctgcagggaa ggccggaaat aggccataga ggtctcgcgg
     1681 gcccggagca gaagcatggg cagagactca ctgaatttgc gtggcgcaag gctccggcct
     1741 tccaagatga gcgattattt aacatgttaa ttccctgtgt tgcatccgca atgccactgg
     1801 aacccgtcgt ctcaacagcc attgagcatt tccgtgacgc gatccatcac gctgtcctta
     1861 tcgtaaagat cgtagtagat cgaggccatg accggctggg gaccgaagaa aaagcgctcg
     1921 tagagcacct gacggttgcc gaatcctgag accgcgacgt cgtgcgccag ccggaacagg
     1981 gcaacgcggt ccctgctctc cagattggtg agctgaaagt attgctccac gtccccggca
     2041 atggcgtttt cgaaatcggc ctcgcagggc gtggccatga gcgaactaga gccgagcatg
     2101 tgcacggttt ccacgagctt cggataaagc ctcgggaaca ggttacggga cgtatccaga
     2161 ggcaggcgct tgggtaccca caggccccag cgatcgggtt ctgcctgctg ctcggcgctg
     2221 aacagaaagg cccggacggt ttcgactatc cacatcgctt ccgcgatctg gcctttgacg
     2281 ttggcatcct tgtctttgcc cgaggccttg gccatggcgc agagcagacc cacgatgaac
     2341 tccgccttgg ccagcttccc gcaggccacc tgatgcatca tgtgcacgac ggcgttggtc
     2401 tccgcaaacg cgcggttgca cagcagcggc gcctggtaca tgaacacacg ctcccagggc
     2461 accaacacgt tgtcgaagat gaccaccgcg tccatttcct cgaatcggga tgccagaggc
     2521 gcatcgaaat ggctcttgcc cgcgtcgtag gtgtcccgac agatgagccg caggcccctg
     2581 gtgtcgatgg g
//
LOCUS       DBNHQR010000540         7223 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_307304, whole genome shotgun sequence.
ACCESSION   DBNHQR010000540 DBNHQR010000000
VERSION     DBNHQR010000540.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7223)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7223)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7223
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_307304"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="AC16VR_12305"
     CDS             complement(<1..959)
                     /locus_tag="AC16VR_12305"
                     /inference="COORDINATES: protein motif:HMM:NF014805.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6011983.1"
                     /translation="MSRPISRILVANRGEIARRIIRTAHELGIGTVAVYADADADAPY
                     VREADTAIALGGRSSTETYLDIPKVIEACRQSGADAVHPGYGFLSENERFAQAVVDEG
                     VRWIGPAPRVIGLMGDKLSAKRLMVEAGVPTLPAVEITEATDLAEAASQIGYPVLVKA
                     SAGGGGRGMRVVEDEGGLADAVLGAKREAASSFGDDTVFFEKWLESSRHVEVQILGDG
                     HGNVLHCFERECSIQRRHQKIIEEAPSPAVTPDLRARMGAAAVAAAKKLGYTSAGTVE
                     FLLSGDEFYFLEVNARLQVEHPVTEAVIGWDLVREQIRVAEGER"
     gene            complement(956..2587)
                     /locus_tag="AC16VR_12310"
     CDS             complement(956..2587)
                     /locus_tag="AC16VR_12310"
                     /inference="COORDINATES: protein motif:HMM:NF013225.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR6011984.1"
                     /translation="MPVLESSLDTRAEAFVRNKADMQQALEEFRALLDEAAEGGGAEA
                     AARLASRGKMPIRERISFVLDRDSPFLEISPLAAWRSAYAVGSGFVVGIGVIEGVECV
                     ILGHDPSVRAGAFNPFNSKKLMRGLEIARINRMPYVQFVESAGADLRGETGEDPEAAI
                     RREGDHFAESGRLFYEITELSKMRIPTISVVFGASTAGGAYQPGMSDYNIFIKNQSRV
                     FLGGPPLVKMATGEDANEESLGGADMHTQVSGLGDYLALDELDGIRMCREVVKHLNWR
                     KLGPEPRADFAEPLHDPEDLLGMVSKDLRSPFDMREVIARIVDASYFEEFKPLYGPTL
                     VCGWASIHGYPVGILGNNGALFSESSEKAAQFIQLCNQIDVPLLFLHNITGFIVGTDF
                     EQGGITKNGSKMINAVANSTVPHITVIVGASYGAGNYGMSGRAFGPRFTFIWPFAKIA
                     VMGPKQMAGVMSIVGRAAAARRGIEFDEEADASRAAVAEHWAEERSKGLYATSRVSDD
                     GMIDPRDTRTVVAIALSACFNNRVEGTKEFGTWRM"
     gene            2702..3901
                     /locus_tag="AC16VR_12315"
     CDS             2702..3901
                     /locus_tag="AC16VR_12315"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6011985.1"
                     /translation="MTTDQQLADFEHQVDGWFAENKPADPGFLLPETFMEVGTDQQFE
                     FLRDWQRKVYEAGYVGMAWPKAYGGGGLPQIYQDIATRAMARHRVPFLPNTIGLNWAG
                     PLILHMGSEEDKQRYIKGILSGDDIWCQGFSEPDHGSDLGNAQCRATRDGDEYVINGS
                     KIWTSLGTYAKYMILLARTATGGSSKYAGLSFFLCPMDAPGIEPRPIQKLTGEYGFCQ
                     TFFDEARIPATCLMGQEGDGWRIAMITLTFERGATGGQAGGLSAMDLNINDVVELARR
                     ARRDGGPALADPLIQDELVQLMIESRGNALMADRTTIPALASDWPEAIAMSGKLRATE
                     LKRRMCRFALAMQGGNGARYVSADAVDGGMWQRSYLNAFSGTIGGGTSEIQRNILGER
                     VLGLPKD"
     gene            3898..5034
                     /locus_tag="AC16VR_12320"
     CDS             3898..5034
                     /locus_tag="AC16VR_12320"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6011986.1"
                     /translation="MSTTVASIEFSDEQSLLLETAASFCRDRSPIERVRAGLGDENGC
                     DAAVWQEMVDLGWLGIAAPLEHGGSGLGLAGVVPVVECMGRHLMGSPYVATIQAIEAL
                     LGSASDEQLAQWLPTILAGAPATVALMEPDGSWDVRAATASGESTGNAIRLRGTKTFV
                     LDAAAAELIVTSIDLDGETRLVALRRDQLPDGALNRETVIDETRRSYGLVLDGIRVPR
                     SQILPGSDFERIERAALLLVCAEIAGGLCGVLHTIVGYLTTRRQFDRLIGSYQALKHP
                     TAEILLAAERSKSHLYHAATTIGRGGREAEVALRMAKAQGSEAFAWAGDRAVQFHGGF
                     GFTYECDAQLFLRRALWCQYQLGDERYHRRKLAPLLLAATDTEH"
     gene            5039..6040
                     /locus_tag="AC16VR_12325"
     CDS             5039..6040
                     /locus_tag="AC16VR_12325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR6011987.1"
                     /translation="MKAITSITGIDLNEVGPLTRDLEARGFSGVSTQENRFNPFLPLA
                     VAATQSSTLELRTSVAIAFARSPMVSANLGWDLQAASRGRFTLGLGSQVKGHNVRRFS
                     VPWSPPAPRLREYVRALRAIWDCWQNGTPLDFAGEHYQFNLMTPNFTPPPLATAPPRV
                     QLAGVGPATMKVAAEECDGVMLHGFCTRRYVEEAVMPRLETGLAAAGRAREDFEISGG
                     GFVATGKDDAAVAEMFEWVRTRVGFYGSTPSYWPVFEVHGLEDLGHKLNDMSKLGQWD
                     AMAGEISDDVVRLFAAVGRFDEIAGAIEERFGGAVDVVALPASAPPDLLEDIARIAC"
     gene            complement(6069..6803)
                     /locus_tag="AC16VR_12330"
     CDS             complement(6069..6803)
                     /locus_tag="AC16VR_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008570400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HIR6011988.1"
                     /translation="MAHEIDYRICGESMQYLEVELDPGESVVAEAGTMMYLSEGIAYE
                     ARLGDGSASGVVGKLFGAAKRLLGSESLFMTHFTNEADGPRQMGFAAPYPGQVVAVDL
                     AQAGGEIVCQKDAFLCAEMGTSVSVAFSKRLGAGFFGGEGFILQRLAGDGTAFIHAGG
                     TVLEKRLEGEKLRVDTGCIVGFEPGIDYDIQLAGGLKSMMFGGEGLFLATLEGHGRVW
                     LQSLPFARLADRVLENAVHLERKGES"
     gene            6960..>7223
                     /locus_tag="AC16VR_12335"
     CDS             6960..>7223
                     /locus_tag="AC16VR_12335"
                     /inference="COORDINATES: protein motif:HMM:NF051574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7884 domain-containing protein"
                     /protein_id="HIR6011989.1"
                     /translation="MKTKIFDRFLTNGTLAIVDADGSSRQFGCGAPFATMRLNRRGTL
                     TRILRNPELNLGETYLAGEWDVIEGSLHDLLTSLRTNLERHAGR"
BASE COUNT         1273 a         2472 c         2279 g         1199 t
ORIGIN      
        1 cgctccccct cggcaacacg aatctgctcc cgcaccagat cccaaccgat cacggcctcc
       61 gtgaccgggt gctccacctg aagccgcgcg ttcacctcca gaaagtagaa ctcgtcgccg
      121 ctcagcagaa actccaccgt tccggcagac gtgtagccga gcttctttgc ggccgccacc
      181 gcggccgcac ccatgcgggc tcgaagatcc ggcgtgaccg ccggcgaggg cgcttcctcg
      241 atgatcttct ggtggcgccg ctggatggag cactcgcgct cgaagcagtg cagcacgttt
      301 ccgtgaccat caccgagaat ctgcacctct acgtgtcgcg agctctccag ccacttctcg
      361 aaaaacaccg tatcgtcacc gaaggaggac gcggcttcgc gcttcgcgcc cagcaccgcg
      421 tcggccagcc cgccctcgtc ctcgacgacc ctcatgcccc gcccgccgcc gcccgcggaa
      481 gccttcacca gcaccgggta gccaatctgc gatgcggctt ccgcaagatc cgtcgcctcg
      541 gtgatctcca ccgcaggcag ggtaggcacg ccggcttcta ccatgagccg ctttgccgac
      601 agcttgtccc ccatgagccc gatcacgcga ggggccggcc cgatccaccg caccccctcg
      661 tccaccacgg cctgggcgaa acgctcgttc tccgagagaa atccataccc ggggtgcacg
      721 gcgtcagcgc cgctctggcg acaggcttcg atgacctttg ggatgtcgag ataggtctcg
      781 gtactggaac ggcccccgag cgcaatggcc gtatccgcct cgcgcacgta tggcgcatcc
      841 gcgtccgcat ccgcgtacac ggcaacggtg ccgattccga gctcgtgggc cgtgcggatg
      901 atgcgacggg cgatctcgcc gcgattagcg acgaggatcc gcgagatcgg cctgctcaca
      961 tccgccacgt gccgaactcc ttggtccctt ccacccgatt gttgaagcag gcagacaggg
     1021 caatcgcgac cacggtgcga gtgtcccggg ggtcgatcat gccgtcgtcc gagacccggg
     1081 aggttgcgta cagccccttg gagcgctctt cggcccaatg ctcggcgacg gccgcccggc
     1141 tcgcatcggc ctcctcgtcg aattcgatgc cacgccgggc cgccgccgcg cggccaacga
     1201 tgctcatcac gcccgccatc tgcttcgggc ccatgacggc gatcttggcg aacggccaga
     1261 tgaaggtgaa acggggcccg aacgcgcgcc cgctcatgcc gtagttgccc gctccgtacg
     1321 aggcgccaac gatgacggtg atgtgcggaa ccgtggagtt ggcaacggcg ttgatcatct
     1381 tcgaaccgtt cttggtgatg ccgccctgct cgaaatccgt gcccacgatg aagccggtga
     1441 tgttgtggag gaacagcagc ggcacgtcga tctggttgca cagctgaatg aactgggccg
     1501 ccttctccga agattccgag aagagcgcgc cgttgttgcc caggatgccg acggggtagc
     1561 catgaatgct ggcccagcca cacaccaggg tgggcccgta caacggcttg aactcctcga
     1621 agtaggaggc gtcgacgatg cgggcgatga cttcgcgcat gtcgaagggc gagcgcagat
     1681 ccttggagac catgcccagc agatcctcgg gatcgtgaag gggctcggcg aagtcggctc
     1741 tcggctcagg gccgagcttg cgccaattga gatgtttgac cacttcccgg cacatacgga
     1801 tgccgtccag ctcgtcgagc gccaggtaat cgcccaaccc ggatacctgg gtgtgcatgt
     1861 cagcgccgcc gaggctctct tcgttcgcat cctcgccggt ggccatcttc accaggggcg
     1921 gtccgccgag aaagacccgc gactggttct tgatgaagat gttgtagtca ctcattcccg
     1981 gctggtaagc gcccccggcc gtggacgcgc caaagaccac cgatatggtg gggatgcgca
     2041 tcttggaaag ctcagtgatc tcgtagaaca gccgcccgga ctccgcgaag tgatccccct
     2101 cccggcggat ggccgcctcc gggtcctccc cggtctcgcc acgcaggtcg gcacccgcgg
     2161 actccacgaa ctgcacatag ggcatgcgat tgatccgcgc gatctccagc ccgcgcatga
     2221 gcttcttcga gttgaacggg ttgaaggcac cggcgcgcac ggaagggtcg tggccgagaa
     2281 tgacgcactc cacgccctcg atgacgccga tgcccaccac gaaaccggac ccgacggcgt
     2341 acgcggagcg ccaggccgcc agcgggctga tctccagaaa cggcgagtca cgatcgagaa
     2401 cgaatgaaat ccgctcgcga atcggcatct ttccgcgtga ggccagccga gccgcagctt
     2461 ccgctccgcc gccctcggcg gcttcgtcca gcagagcccg gaactcttcg agagcctgct
     2521 gcatgtcggc cttgttgcgc acgaacgcct cggccctggt gtccaggctc gattccaata
     2581 ccggcatgcg gtccccccga tccgtcgagg ccactttatg ccagaacttt ctcgtcagaa
     2641 caaaacgtgc cgggagaggc gtttcgttgt gctagattga gcccgcgacg ccggaggaga
     2701 gatgacgacg gaccagcaac tcgccgattt cgagcaccag gtggacggct ggtttgccga
     2761 gaacaagccc gccgacccgg gctttctgct cccagagacc ttcatggagg tcggcaccga
     2821 tcagcagttc gagtttctcc gcgactggca gcgaaaagtc tacgaggccg gatacgtggg
     2881 gatggcctgg cctaaggcct acggcggggg cggcctcccg cagatctacc aggacatcgc
     2941 gacccgggcc atggcccgcc accgggttcc cttcctgccg aacaccatcg gcctgaactg
     3001 ggcagggccg ctgatccttc acatgggatc ggaagaggac aagcagcgct acatcaaagg
     3061 cattctcagc ggggacgaca tctggtgcca ggggttctcg gaacccgacc acggctcgga
     3121 tctgggcaac gcccagtgtc gggcgacgcg cgacggcgat gagtacgtga tcaacggctc
     3181 caagatctgg accagtctcg gcacctatgc caaatacatg atcctgctcg cccgaacggc
     3241 caccggcgga agcagcaagt acgcgggcct gagctttttc ctttgcccga tggatgcgcc
     3301 cggcatcgag ccgagaccca tacagaaact caccggcgag tacggtttct gccagacgtt
     3361 cttcgacgag gctcgaatac ccgccacctg cctgatgggg caggaaggcg atggctggcg
     3421 catcgccatg atcacgctca cgttcgagcg cggcgcgacg ggcggccagg ccggcgggct
     3481 gtccgccatg gatctcaaca tcaacgacgt ggtggagctg gccaggcgcg ctcgtcgcga
     3541 cggcggtccg gcgctggcgg atccgctgat tcaggacgag ctggtgcagc tgatgatcga
     3601 gtcccggggg aatgccctga tggcagaccg cacaaccatc cccgcgctcg cctccgactg
     3661 gcccgaagcc attgccatgt cgggcaagct gcgcgccacc gagctcaaac gccgcatgtg
     3721 ccgattcgcg ctggccatgc agggcggcaa cggcgcccgc tacgtgagcg cggatgccgt
     3781 ggatggcggc atgtggcagc gctcctacct gaacgcgttc tcgggcacca tcggcggggg
     3841 cacgtctgaa attcagcgca acatcctcgg agagcgggta ctcggcctgc cgaaggattg
     3901 agcaccaccg tggcgtccat cgaattctca gacgagcagt cgctgctcct ggaaaccgcc
     3961 gcctcgttct gtcgcgatcg atccccgata gagcgggtgc gcgccggcct cggcgacgaa
     4021 aacggctgcg acgccgccgt gtggcaggag atggtagacc tcggctggct ggggatcgct
     4081 gccccgctgg agcacggcgg ttcagggctc ggtctggcag gcgtcgttcc ggtggttgag
     4141 tgcatgggcc gccacctgat ggggtcaccc tacgtcgcca ccatccaggc cattgaagcg
     4201 ctgctgggct cggccagcga cgagcagctg gcgcagtggc tgccgacgat actggccggg
     4261 gcgccggcaa ccgtggcgct gatggagcct gacggcagct gggacgtccg cgcggccact
     4321 gccagcggag agtcgacggg aaacgccatt cgcctgcgcg gcaccaagac cttcgtgctg
     4381 gacgcggcgg ccgccgagct gatcgtgacg tccatcgacc tcgacggcga aacccggctg
     4441 gtggcccttc gccgcgatca gctgcccgat ggggcgctca accgcgagac ggtgatcgac
     4501 gagacccgcc gcagctatgg cctggtcctg gacggcatca gggtaccgcg atcccagatt
     4561 ctgccgggca gcgatttcga acggatcgag cgggcggccc tgctcctcgt ctgcgcggag
     4621 atcgccggcg ggctttgcgg cgtgctgcac accatcgtcg gctacctgac cacgcggcgc
     4681 cagttcgacc gcctcattgg cagctaccag gcgctcaagc accccacggc cgagatcctg
     4741 ctcgcggccg agcgcagcaa gtcacacctc taccacgcag ccaccacgat cggccggggc
     4801 gggcgcgaag ccgaggtcgc cctgcggatg gcgaaagcac agggaagcga ggccttcgcg
     4861 tgggccggtg accgggcggt gcagtttcac gggggcttcg gcttcaccta cgagtgcgac
     4921 gcccagctgt ttctgcgccg ggcgctatgg tgccagtatc agcttggtga cgaacgctat
     4981 catcggcgca agctcgcgcc gctactccta gcagcgacag acacggagca ttgagctcat
     5041 gaaggccatt acctccatca ccggcatcga cctcaacgag gtcgggcccc taacccggga
     5101 cctcgaagcg cgcgggttca gcggcgtgtc cacccaggaa aaccgtttca acccctttct
     5161 cccgctggcc gtggcggcca cccagtcgtc gaccctggag ctgagaacca gcgtggcgat
     5221 tgccttcgcg cgcagcccca tggtttccgc caatctcggc tgggacctgc aggcggcttc
     5281 ccgcggccga ttcacgctcg gcctcggcag tcaggtcaaa gggcacaacg ttcgccgctt
     5341 cagcgttccc tggagcccgc cggcgccaag gctgcgcgag tacgtccgcg ctcttcgggc
     5401 catctgggac tgctggcaga acgggacccc gctcgatttc gccggcgagc actaccagtt
     5461 caacctgatg acgccgaact tcaccccacc gccgctggcc accgccccgc cccgggtgca
     5521 gctggccggc gtcgggcccg ccacgatgaa ggttgcagcc gaggagtgtg acggggtcat
     5581 gctgcatggg ttctgcacgc gccgatacgt ggaggaagcg gtgatgccgc gcctcgagac
     5641 gggcctggcc gccgccgggc gcgcgcgcga ggacttcgag atctccggcg gtgggttcgt
     5701 cgccacgggc aaagacgatg ctgccgttgc ggagatgttc gagtgggtcc gaacccgcgt
     5761 gggcttttac ggttccaccc cctcctactg gcccgtattc gaggtgcacg ggctcgagga
     5821 tctcggccac aagctgaacg acatgtccaa gctcggccag tgggatgcca tggccggaga
     5881 aatatctgac gacgtggtcc ggctcttcgc cgcggtgggc cgtttcgacg agatcgctgg
     5941 tgccatcgag gagcgctttg gcggcgccgt ggacgtggtt gccctgcccg cctcagcccc
     6001 gcccgacctg ctcgaggaca tcgcccggat cgcttgctga gtccccctca ggagccgccg
     6061 gatccggttc aggactcgcc tttgcgttcc aggtgcacgg cgttctccag gaccctgtcg
     6121 gccagccgcg cgaacggcag ggattgcagc catacccgtc catgcccctc cagagtggca
     6181 agaaacaggc cctcgccacc gaacatcatg gatttcaggc cgccggcaag ctggatgtcg
     6241 tagtcgatgc ccggctcgaa gccgacgatg cagccggtgt ccacgcgaag cttctcgccc
     6301 tcgagacgtt tttctagcac ggttcccccg gcgtgaatga acgccgtgcc gtcaccggcc
     6361 agacgctgaa ggataaagcc ctcgccaccg aagaagcccg caccaagacg cttgctgaac
     6421 gccacggaca cggaggtgcc catctccgcg cagagaaaag cgtccttctg acagacgatc
     6481 tcgccacctg cctgtgccag atccaccgcg accacctgcc cgggatacgg agccgcaaag
     6541 cccatctgcc gcgggccgtc ggcttcgttg gtgaagtgcg tcatgaacag cgactcgctg
     6601 ccgagcagcc gtttggccgc accgaagagt ttacccacca ccccgctggc cgacccgtcc
     6661 ccgagccgtg cctcgtaggc aatgccctcg ctcagataca tcatggtgcc cgcttcggcg
     6721 accacagact ccccgggatc gagttctact tcgaggtact gcatggattc accgcaaatc
     6781 cggtagtcga tctcgtgcgc catcgcgcgg tcctcctcgt tctcaagccc cgacctgcac
     6841 cttctggccc ccggcgacgc caaaaggtct agttgaaaaa acacccgcag cgcgataatt
     6901 attgccatgt gaggggatgg ctggcaaaga accggccgcc gagcaggggg catgacgtcg
     6961 tgaaaacaaa gatcttcgat cggtttctca ccaacgggac gctggccatc gtcgacgccg
     7021 atggttcaag ccgtcagttc ggctgcggcg ctccgttcgc cacgatgcgc ttgaaccggc
     7081 gcgggacgtt gacccggatt ctgcgcaatc ccgagctcaa cctgggcgag acctacctgg
     7141 caggcgagtg ggacgtcatc gagggctctc tgcacgatct gctcaccagt ttgcgcacga
     7201 atctcgagcg tcacgccggt cga
//
LOCUS       DBNHQR010000541         2655 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1321904, whole genome shotgun sequence.
ACCESSION   DBNHQR010000541 DBNHQR010000000
VERSION     DBNHQR010000541.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1321904"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="AC16VR_12340"
     CDS             complement(<1..1038)
                     /locus_tag="AC16VR_12340"
                     /inference="COORDINATES: protein motif:HMM:NF013740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR6011990.1"
                     /translation="MSTEQDTDVIRTIVSQDLLDVLVRVGLIGILLVMSFRIFSPFLS
                     ILLWALVLAVSLYPVHLKLVGKMGGRQGASATVIVIVGMLLIGTPTIALGSLFAGYIH
                     ETYQAFATNSVTVNPPRPEVAEWPIIGERMYAAWSAAASDMPAFLEELQPQLGVLTDR
                     ALSTAASTMGDVLLFLGALIIAGIMMAFGQSGSDAMGRILSRLSGPENGPRLHNLTTA
                     TIRSVATGVIGVAFIQALLLGVGFAFAGIPAAGLLALVVMLVGIVQLPALLVTLPVIA
                     YLWWGGDASTTSNVIWTIYLLVAGMADNVLKPLLLGRGVDAPMPVILIGALGGMVSAG
                     IVGLFVGAVILA"
     gene            complement(1105..>2655)
                     /gene="selD"
                     /locus_tag="AC16VR_12345"
     CDS             complement(1105..>2655)
                     /gene="selD"
                     /locus_tag="AC16VR_12345"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HIR6011991.1"
                     /translation="ALDCRHGLLESSDGRSTAADHAFWVTGVMAASWPAAAGLATDVS
                     GFIEVDRCLRSTSHESVYAAGDVACLIDQPRPKSGVYAVREGPALTRNLRRAIVGRPG
                     IPFRAQKRFLSLIGTADGSAVAARGPWAASGRWAWRWKDWIDRRFVARFNRLPVMTEP
                     GWSLPESLRDEAPDAMRCGGCGAKLGASPLMRVLERLPPQVSGNVRVGIGDDAAILGA
                     TGGETILTVDGFRSLVSDPYLFGRITAHHALNDIIAMGGKGTAALAMASVPLMSEALM
                     EEDLFQLLRGAVDVLNAHEVPLVGGHSSEGAELSLGLTVAGVPDGPTLTKGGAEPGDA
                     LVLTKPLGTGSILAGQMRGAVRSDSVSAAIASMDASNASALVVFRRYGIHAATDVSGF
                     GLLGHLGEMLRASALGVRLDLNAVPFIEGAVEAVSAGAVSSLQDNNELALADFALEGC
                     EPGDATVRLLVDPQTSGGLLGCVPEAEAEACVRDLREVGYPRAAVIGRVLSNGWTVTA
                     EREARPLS"
BASE COUNT          505 a          943 c          833 g          374 t
ORIGIN      
        1 cgccaggatc acggcgccga cgaacagccc cacgatgccg gcggacacca tgccgcccag
       61 cgcgccgatc agtatcaccg gcatgggcgc gtcgacgccg cgacccagca gcaggggttt
      121 gaggacattg tcagccatac cagctacgag caggtagatc gtccatatga cgttgctggt
      181 cgtggacgca tcgccgcccc accacagata ggcgatcacc ggaagcgtga ccagcagcgc
      241 gggcagctgg acgatgccga ccagcatcac caccagcgcc agcaaacctg cggctgggat
      301 gcccgcaaag gcgaatccca cgccgaggag cagggcctga atgaacgcca cgccgatcac
      361 gcccgtggcc accgagcgaa tcgtcgccgt cgtcaggttg tgcaggcggg gcccgttttc
      421 cgggccggac agccgactga ggatccgacc catggcgtcg ctgcccgact ggccgaacgc
      481 catcatgatg cccgctatga tgagggcacc gagaaagagc agcacatcgc ccatggtgct
      541 ggccgccgtg ctcagcgcac gatccgtcag aacgccgagc tggggctgca attcctccag
      601 gaaggcaggc atgtcgcttg ccgccgccga ccaggccgca tacatccgct ccccgatgat
      661 gggccactcg gcgacctccg gcctcggtgg gttgacggtg accgagttgg tcgcaaacgc
      721 ctgataggtt tcgtggatat agccggcaaa aaggctgccc agggctatgg tcggcgtgcc
      781 gatcagcagc atcccgacaa tgacgatcac ggttgccgac gcgccctgcc gaccacccat
      841 cttgccgaca agtttcagat gcaccgggta cagtgacaca gcgagaacga gcgcccacag
      901 gagaatgctg agaaacggag aaaatatccg gaagctcatc accagaagga tgccgatcag
      961 ccccacccgg accagcacgt ccagcaagtc ctgggaaacg atggtgcgga tgacgtcggt
     1021 gtcctgctcg gtgctcattt ccactccccc aactgatgcc aaacggcgaa tcatacgcga
     1081 ggacgcgcat ggtcgtctcg cggctcagct gagcgggcgc gcctctcgtt cggcggtaac
     1141 ggtccagccg ttcgacagca cccggcctat gacggccgca cgcgggtaac cgacctcgcg
     1201 cagatcccga acgcaggctt cggcctccgc ctccggcacg cacccgagca gcccgccgga
     1261 agtctgaggg tcgacgagca gacgcacagt cgcatcgccc ggctcgcagc cttccagcgc
     1321 gaaatccgcg agggcgagct cgttgttgtc ttgcagcgag ctgacggcac cggcgctgac
     1381 ggcctcgacc gcaccctcga tgaaaggcac cgcgttgagg tcgagccgca cacccagggc
     1441 ggacgccctg agcatctccc ctaggtgacc gagcaacccg aagcccgaca cgtcggtggc
     1501 ggcatggatg ccgtagcgac ggaacacgac cagagccgat gcgttcgatg cgtccatgct
     1561 ggcaatagcg gcggagacgc tgtccgagcg aacggcgccg cgcatctgcc cggccaggat
     1621 cgaacccgtg ccgagcggct tggtgagcac cagcgcgtcc cccggttccg ccccgccctt
     1681 ggtcaaggtc ggtccgtcgg gaacgcccgc aacggtaaga cccaggctca gctcggctcc
     1741 ctcactcgag tgcccgccca ccaggggcac ctcgtgcgca ttgagcacgt ccaccgcgcc
     1801 gcgaagcagc tgaaagagat cttcctccat gagcgcttcg gacatcagag gcacgctcgc
     1861 catcgctagg gcagccgtac ccttaccgcc catggcgatg atgtcgttca aggcgtggtg
     1921 ggccgttatg cggccaaaca ggtaagggtc ggagacgagg ctgcggaagc cgtcgacggt
     1981 gagtatcgtc tcgccaccgg tcgcgccgag gatggccgcg tcatcgccga tgccgacccg
     2041 cacgttcccg gacacctgag gcggcaggcg ctcgagcacg cgcatcagcg ggctcgcgcc
     2101 gagcttcgcc ccgcagcctc cgcatcgcat ggcgtccggc gcttcgtcac ggagactctc
     2161 tggcagcgac cagcccggtt ccgtcatgac cggcagccgg ttgaagcgcg ccacgaaccg
     2221 gcggtcgatc caatccttcc atcgccaggc ccagcggccg ctggccgccc aagggccgcg
     2281 agcggcgaca gcggacccgt cggcagttcc gatgaggctc aagaagcgct tctgtgcccg
     2341 gaacggaatc ccgggccgtc cgacgattgc ccgccttaga ttgcgggtca gcgccgggcc
     2401 ctcccgaacg gcgtacacgc ccgacttggg cctcggctga tcgattagac aggccacgtc
     2461 cccggccgcg taaacgctct catgactcgt ggaccgcagg cagcgatcaa cctcgatgaa
     2521 cccgctgaca tccgtggcca gcccagccgc agccggccac gacgctgcca tcaccccggt
     2581 aacccagaac gcatggtccg ccgccgtcga ccgcccatcg gagctttcga gcagcccgtg
     2641 cctgcaatcc agcgc
//
LOCUS       DBNHQR010000542         9425 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_940197, whole genome shotgun sequence.
ACCESSION   DBNHQR010000542 DBNHQR010000000
VERSION     DBNHQR010000542.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9425)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9425)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9425
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_940197"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="AC16VR_12350"
     CDS             <1..242
                     /locus_tag="AC16VR_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016490546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="uL15 family ribosomal protein"
                     /protein_id="HIR6011992.1"
                     /translation="FRSRVGMTTAEVRLGEIGKVEGDVVDLDALKAANVLSRNVKRAR
                     IMQSGRVERPLTVKGLIVTKGARSAIEAAGGKVED"
     gene            242..1573
                     /gene="secY"
                     /locus_tag="AC16VR_12355"
     CDS             242..1573
                     /gene="secY"
                     /locus_tag="AC16VR_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014869934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIR6011993.1"
                     /translation="MATNAGQLTGVQAGLSELRSRLLFVLIALLVYRMGTHIPVPGIN
                     PDRLAALFDQQQGGILDLFNMFSGGALERMSILALGVMPYITSSIIMNLMTMMYPSLQ
                     QLRKEGESGRRKITQYTRYGCLLIALIQGTSLSVTLANQGLAFSANFAFFFVATTTLV
                     TGALFMMWLGEQITERGVGNGISLIIFSGIVSGFPAAIGQSFEAARQGDINIIVLLFI
                     AALAVAIVGFVVFVERGQRRITVNYAKRQQGRRVFQGQSSHLPLKINMAGVIPAIFAS
                     SLLLAPASMAQWFGNSPGMDWLQQIALVLSPGQPLYILMFAAGIIFFSFFYTAIMFDP
                     KDMADNLKRQGAYVPGIRPGQQTARYIDDVITRLTVFGALYVTMVCLLPEFMIVAFNV
                     TFQLGGTALLIVVVVAMDFMSQVQAHLMSSQYAKLMEKSNLKGYGRSGRPR"
     gene            1583..1699
                     /gene="rpmJ"
                     /locus_tag="AC16VR_12360"
     CDS             1583..1699
                     /gene="rpmJ"
                     /locus_tag="AC16VR_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HIR6011994.1"
                     /translation="MKVRASVKRMCRNCKIIKRNGVVRVICSAEPRHKQRQG"
     gene            1714..2070
                     /gene="rpsM"
                     /locus_tag="AC16VR_12365"
     CDS             1714..2070
                     /gene="rpsM"
                     /locus_tag="AC16VR_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HIR6011995.1"
                     /translation="MARIAGINIPDNKHAVVSLTYIFGIGRTTAAKICEAAGVNPSSK
                     IQDLDEANLDAIRAEVAKFTVEGDLRRAVSMNVKRLMDLGCYRGIRHRRGLPVRGQRT
                     RTNARTRKGPRRPIRK"
     gene            2073..2447
                     /gene="rpsK"
                     /locus_tag="AC16VR_12370"
     CDS             2073..2447
                     /gene="rpsK"
                     /locus_tag="AC16VR_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HIR6011996.1"
                     /translation="MAEKKKAKRVVIDGVAHVHASFNNTIITISDRQGNALCWATAGG
                     SGFRGSRKSTPFAAQVAAERVSNTALEHGMKSVDVYIKGPGPGRESAVRALNAAGLRI
                     NSIADVTPIPHNGCRPPKRRRV"
     gene            2472..3092
                     /gene="rpsD"
                     /locus_tag="AC16VR_12375"
     CDS             2472..3092
                     /gene="rpsD"
                     /locus_tag="AC16VR_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005781973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HIR6011997.1"
                     /translation="MARYIGPKLKLSRREGTDLFLKSGVRPIDSKCKLEQPPGQHGAR
                     RGRLSDYAIQLREKQKVRRLYGVLEKQFRNYYKKAERQKGATGENLLRLLECRLDNVV
                     YRMGFGATRAESRQLVSHKAITVNGSLVNIPSYQVAADDVVGVYEKARGQLRIQSAMQ
                     LASQRAPVEWVDVDPTKLEGVYRRPPDREELPPEINENLIVELYSK"
     gene            3140..4135
                     /locus_tag="AC16VR_12380"
     CDS             3140..4135
                     /locus_tag="AC16VR_12380"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018403735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HIR6011998.1"
                     /translation="MAQSVTEFLTPRHIDIEASSPTRAKVTLEPLERGFGHTLGNTLR
                     RILLSSMPGCAITEVQIDGVLHEYSTLEGVQEDVINILLNLKGIAVILHSQDEAVITL
                     NKDGAGQVTAGDFQLTQDVEIANPDHLICSLNDNGAIRLEARVTRGRGYVPADSREEE
                     EDSRPIGVLSLDATFSPIRRVAYGVESARVEQRTDLDKLVLDLETNGTIEPEEAIRRA
                     ATILQQQLAVFVDLESEGEPVAEEKEDEIDPILIRPVDDLELTVRSANCLKAENIYYI
                     GDLIQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASLRGSDKVA"
     gene            4226..4672
                     /gene="rplQ"
                     /locus_tag="AC16VR_12385"
     CDS             4226..4672
                     /gene="rplQ"
                     /locus_tag="AC16VR_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HIR6011999.1"
                     /translation="MRHRKSGRHLNRTSSHRQAMFRNMAASLIEHEVIKTTLPKAKEL
                     RRVAEPLITLAKEDSVANRRLAFARTRSDAAVGKLFVELGPRYKDRPGGYTRILKCGN
                     RAGDAAPMAFIELVDRPVVDEFDEDEFDAADEQADEAEELEAEEKG"
     gene            complement(4690..7515)
                     /gene="uvrA"
                     /locus_tag="AC16VR_12390"
     CDS             complement(4690..7515)
                     /gene="uvrA"
                     /locus_tag="AC16VR_12390"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR6012000.1"
                     /translation="MDSIAVRGARTHNLKNVNLDIPRDRLVVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSMMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIYDYLRLLYARVGEPRCPNHDLPLDAQTVSQMVDQVLGLPEDKRVMVLAPVVTERKG
                     EHLHVFQELMANGFIRARIDGIVVDLDHPPNLEKNKKHTVEAVIDRLRIRADAQQRLA
                     ESFETALDLAEGIAKVASMDDADEPELVFSARFACPACGYSISELEPRMFSFNNPAGA
                     CQACDGLGVKQFFDPGLVVQDEELTLAEGAIRGWDRRNVYYFHMLSSLAAHYGFDVET
                     PFRKLPKKHRDAILYGSGRERIDFSYANDRGDVIHRRHRFEGVIPNMERRYHETDSSL
                     VRENLQKYLRVRSCPDCGGTRLREASRNVFIDGVSLPEITSGSVEGSLAYFDELKLSG
                     RRGEIADKILKEIRARLRFLVDVGLNYLTLDRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYILDEPSIGLHQRDNARLLKTLEHLRDLGNTVLVVEHDEEAIRSADHIIDIGPGAGV
                     HGGCVVAAGSLEKILKSRRSITGQYLSGRKKIPVPEKRTAFVEEETIRIIGATGNNLQ
                     SVSVEIPCGLLTCVTGVSGSGKSTLINHTLYPVAATRLNKATTLTPAPVEEVLGLNHV
                     DKVVDIDQSPIGRTPRSNPATYTGLFTPIRELFAQTQEARTRGYKPGRFSFNVKGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDVCKGKRYNRETLDVRYKGRNIHEVLEMTVEEARE
                     FFDAVPMLARKLQTLVDVGLSYIALGQSATTLSGGEAQRVKLSRELSKRDTGKTLYIL
                     DEPTTGLHFHDIAQLLDVLHRLRDQGNTVVVIEHNLDVIKTADWVVDLGPEGGSGGGR
                     VVAAGTPEHLARKRGSHTGRFLKKMLG"
     gene            7725..8168
                     /gene="ssb"
                     /locus_tag="AC16VR_12395"
     CDS             7725..8168
                     /gene="ssb"
                     /locus_tag="AC16VR_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019339955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR6012001.1"
                     /translation="MARGINKVILIGNLGRDPETRYSQGGNAVTSFSVATTENWRDRQ
                     SGEQQERTEWHNVVCFARLAEIAGEYLRKGSKVYIEGSLRTSSWEQDGQKRYRTEVMA
                     RDLQMLDSRGGGQGGQGGGFESAAPAASENAPTLADDDFDDDIPF"
     gene            complement(8165..8758)
                     /locus_tag="AC16VR_12400"
     CDS             complement(8165..8758)
                     /locus_tag="AC16VR_12400"
                     /inference="COORDINATES: protein motif:HMM:NF014269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HIR6012002.1"
                     /translation="MEIPLFPLRVVLFDGGRLPLQIFETRYLDMVSRCTRESIPFGVV
                     LIRGAGSDARLDPLANQPAIFEVGTEAHIVDFNQLPNGRLGIIVRGGRKFRVVSTREQ
                     ADHLLLGTVDFLPAEPDSTVPSEHAPLVDILKELVKHPGVQKLGLEIDFADARSVGAR
                     LAELLPIEPEIKQSLLQLQLPRERLQELTRLVNKFRD"
     gene            8817..>9425
                     /locus_tag="AC16VR_12405"
     CDS             8817..>9425
                     /locus_tag="AC16VR_12405"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIR6012003.1"
                     /translation="MPLDCAAVDWLQIIILALVQGITEFLPISSSAHLILPALLTEWP
                     DQGLAFDIAVHAGTLLAVLSYFRREIGRFAASGARLLARGQRDDDADLLLKLIAATLP
                     IVLVGYLAHDWVESSLRTVPVIASATIVFALALWWADGRRSGADHPSWTAAMLIGAAQ
                     VIAIVPGTSRSGITMTAALLLGLSRTGAARFSFLLSIPTILGA"
BASE COUNT         1752 a         2879 c         3069 g         1725 t
ORIGIN      
        1 gcttccgctc gcgggtcggt atgaccaccg cggaggtgcg actgggcgag ataggtaaag
       61 tcgaaggcga cgtggtcgac ctcgacgcgt tgaaggcggc caacgtgctg tcgcggaacg
      121 tcaagcgggc gcgaatcatg cagtccgggc gcgtggagcg accgcttacc gtcaagggtt
      181 tgatcgtgac caaaggcgcg cgcagcgcca tcgaagcggc cgggggcaag gtagaagact
      241 gatggcgacc aacgcgggtc aactcacggg cgtgcaggcg ggcttgagcg agcttcgctc
      301 ccggctgctg ttcgtcctga tcgcgctcct ggtttatcgc atggggaccc acatcccggt
      361 tcccggcatc aatccggatc gcctggctgc gctgttcgat cagcagcagg gcggcattct
      421 ggatcttttc aacatgttct cgggcggcgc gctggagcgg atgagcatac tcgcgctggg
      481 tgtgatgcct tacatcacct ccagcatcat catgaatctg atgaccatga tgtacccgag
      541 cctccagcag ctgcgaaagg agggggagtc gggacggcgc aagatcaccc agtacacccg
      601 ctacggctgc ctgctgatcg cgctcattca gggcacctct ctttcggtca ctctggcgaa
      661 tcagggcctg gctttctccg ctaacttcgc cttcttcttc gtcgcgacca cgacgctggt
      721 caccggcgcg ttgttcatga tgtggctcgg cgagcagatc acggaacgcg gggtcggtaa
      781 cggcatctct ctcatcatct tctcgggaat cgtctccgga tttccggccg ccatcgggca
      841 gtctttcgaa gccgcgcggc agggcgacat caacatcatc gtgctgctgt tcatcgcggc
      901 attggcggtg gccatcgtcg gtttcgtcgt gttcgtcgag cgcggccagc gccggatcac
      961 agtgaactac gccaagcgac agcagggtcg ccgggtgttc cagggccaga gcagccacct
     1021 gcctctgaag atcaacatgg cgggcgtgat ccccgccatc tttgcttcga gccttctgct
     1081 ggcgccggcc tccatggccc agtggttcgg aaacagcccg ggaatggact ggctccagca
     1141 gatcgccctc gtgctctcgc cagggcagcc gctgtatatc ctgatgttcg cggcgggcat
     1201 cattttcttc agtttcttct acaccgcgat catgttcgat ccgaaggaca tggcggacaa
     1261 cctcaagcgg cagggcgctt acgttcccgg aatccgtccg ggccagcaga cggctcgcta
     1321 cattgatgac gtgatcaccc gcttgaccgt gttcggggcg ctttacgtga cgatggtctg
     1381 tctgctgccg gagttcatga tcgtggcctt caacgtcacg tttcagcttg gcggtaccgc
     1441 cctgctcatc gtggtggtgg tcgccatgga tttcatgtct caggtgcagg cgcatctgat
     1501 gtcgagccag tacgcgaagc tcatggagaa atctaacctc aagggctatg gccgatcggg
     1561 ccggccgagg tagggagtcg ttatgaaagt aagagcatcg gttaagcgga tgtgtcgaaa
     1621 ctgcaagatc atcaagcgca atggtgtggt tcgggtgatc tgcagcgccg agccgcggca
     1681 caagcagcgg cagggctgac taggagcatt tgaatggcac gaattgccgg aatcaacata
     1741 ccggacaaca agcacgcggt cgtatcgctg acctatattt tcgggatcgg tcgcaccacg
     1801 gcggcgaaga tctgcgaggc ggccggggtg aacccctcga gcaagattca ggacctggac
     1861 gaggccaatc tcgacgccat cagggcagag gtggcgaagt tcaccgtgga aggcgacctg
     1921 cggcgtgccg tgtccatgaa cgtcaagcgg ttgatggatc tgggttgcta ccgcgggatt
     1981 cgccaccggc gcgggctgcc ggttcggggt cagagaacgc gcaccaacgc gcgcactcgc
     2041 aaaggtcccc gtcgtccaat caggaaatag atatggctga gaagaaaaag gcgaagcggg
     2101 tggtgatcga cggcgtggcg cacgtgcacg cgtcgttcaa caacaccatc atcaccatca
     2161 gcgaccggca gggcaatgcg ctgtgctggg cgacggcggg cggctcgggc ttccgcggct
     2221 ctcgcaagag cacgccgttt gctgcccagg tcgcggccga gcgcgtatcc aacacggcgc
     2281 tcgagcacgg gatgaaaagc gtggatgtct acatcaaggg tccgggaccg ggccgcgagt
     2341 ccgccgttcg cgctctcaac gcggcgggcc ttcgaatcaa cagcatcgct gatgtgaccc
     2401 ccattcctca caacggctgc cgcccgccga agcggcgtcg tgtctgacga gcaagggaga
     2461 gatagacgac catggctcga tacataggac caaaactcaa gctttcgcgc cgcgagggca
     2521 ccgacctgtt tctcaagagc ggcgtacgcc ccatcgactc gaagtgcaag ctcgagcagc
     2581 ctcccggtca gcatggtgct cggcgcggca ggttgtccga ctacgcgatc cagctgcgtg
     2641 aaaagcagaa agtcagacgc ctttacggcg tcctcgagaa gcagttccgc aactactaca
     2701 agaaggcgga acgacagaag ggcgccaccg gcgaaaacct gctcaggctt ttggaatgcc
     2761 gcctggacaa cgtggtgtat cgcatgggct tcggggcgac cagagcggaa tcacggcagc
     2821 tggtctcgca caaggccatc acggtgaacg gttcgctggt caacatcccc tcctaccagg
     2881 tggccgccga tgacgtggtc ggcgtgtatg aaaaggcacg cgggcagctg cgcatccagt
     2941 cggctatgca gctggccagc cagcgggcgc cggtggaatg ggtggacgtg gacccgacca
     3001 agctcgaggg cgtgtacagg cgcccgccgg atcgggaaga gctgccgccg gagatcaacg
     3061 agaacctcat cgtggagctc tactccaagt aagggcccga agagtaactg aacactccag
     3121 atatcaggag acgaaacata tggcacagtc ggtaaccgaa tttctgaccc cacgccacat
     3181 cgacatagag gccagcagcc cgacccgggc gaaggtgacg ctggagcctc tcgagcgggg
     3241 cttcggtcac acgctgggca acacgctgcg gcgcattctg ctgtcatcca tgccgggctg
     3301 cgcgatcacc gaggtgcaga tcgacggcgt gctgcacgag tacagcaccc tcgaaggcgt
     3361 gcaggaagac gtcatcaaca tcctgctcaa cctgaaaggg atcgctgtga ttctccacag
     3421 ccaggacgag gcggtaatca ccctgaacaa ggacggcgct ggccaggtga cggccggtga
     3481 cttccagctg actcaggacg tggagatcgc gaatccggat cacttgatct gctctctaaa
     3541 cgacaacggc gccattcgcc tggaagcccg tgtcacgcgc ggtcggggct acgtgccggc
     3601 cgacagccgt gaagaggaag aggactcccg gccgatcggt gtgctcagcc tcgacgctac
     3661 cttcagcccg attcgtcggg tcgcctacgg cgtcgagagc gcccgggtcg agcagcgaac
     3721 cgacctggac aagctcgtgc tggatctgga gaccaacggg accatagagc ccgaggaggc
     3781 gatccgccgg gcggcgacca ttctccagca gcagctggcg gtgttcgtgg acctggagag
     3841 cgagggcgag cctgtcgcgg aggagaagga agacgagatc gacccgatcc tcatccgacc
     3901 cgtggacgat ctcgagctca cggtgcgctc ggccaactgt ctcaaggcgg aaaacatcta
     3961 ctacatcggc gacctcattc agcgcaccga ggttgagctg ctcaagaccc ccaatctcgg
     4021 taagaaatca ctgaccgaga tcaaggacgt cctggcgtct cgcggtctgt cccttggcat
     4081 gcgcctggag aactggcctc cggccagtct cagaggcagc gacaaggtcg cctgaagagg
     4141 tcgcctgaag aggtcgagga aggccggagg tggtgatctc gggccgtcgt cgcggcaaag
     4201 gcgcggagta acttaggaag cagtcatgcg acatcgcaag agtggacgac atctcaatcg
     4261 cacgagctca caccggcagg ctatgttccg taacatggcc gcgtcgctca ttgagcacga
     4321 ggtcatcaag acgacgctcc ccaaagcgaa agagctccgg cgcgtcgccg agcctctcat
     4381 caccctcgcg aaggaagaca gcgtcgccaa ccggcgcctg gcattcgcgc gcacgcgcag
     4441 cgacgcggcg gtgggtaagc tgttcgtaga gttgggaccg cgctacaaag atcgcccggg
     4501 cggctacacc cgcatcctga aatgcggcaa tcgggcgggc gatgcggcgc ccatggcgtt
     4561 catcgagctg gtggatcgtc ccgtggtcga cgagttcgac gaagacgagt tcgatgcggc
     4621 ggacgagcag gcggacgaag cagaagagct ggaagccgag gagaagggct gagcctggcg
     4681 cgaaggcacc taccccagca tcttcttgag aaaacgcccg gtgtgagagc cacgctttct
     4741 ggcgagatgc tccggggtcc ctgcggccac cacccggccg ccgccagagc cgccctcggg
     4801 ccccaggtcc accacccagt cggccgtctt gatgacgtcc aggttgtgct cgatcacgac
     4861 caccgtattt ccctgatcgc gcagacggtg cagcacgtcc agcagctggg cgatgtcgtg
     4921 gaagtgaagg cccgtagtgg gctcgtccag gatgtacagg gtcttgccgg tgtcccgctt
     4981 ggaaagctcc ctggacagct tgacgcgttg cgcctcgccg cctgaaagcg tcgtcgcgct
     5041 ctgtccgagg gcgatgtatg acagccccac gtccaccagg gtctgaagct tgcgtgcaag
     5101 catgggaacc gcgtcgaaaa attcccgggc ttcctccacg gtcatctcca ggacctcgtg
     5161 gatgttcctg cctttgtagc ggacgtcgag cgtctcccgg ttgtagcgtt tgcctttgca
     5221 gacgtcgcat ggcacgtata tgtcgggaag gaagtgcatc tccaccttga tgacgccgtc
     5281 accctgacac gcctcgcagc gcccgccctt gacgttgaaa ctgaagcggc cgggcttgta
     5341 accccgggtc ctggcctcct gggtctgagc gaagagctca cggatcggcg tgaacagacc
     5401 cgtatacgtt gccggattgg agcgcggcgt gcggccgatg gggctctggt cgatgtcgac
     5461 caccttgtcg acatgattca gacccagaac ctcctccacg ggtgccggcg tcagcgtggt
     5521 tgctttgttg agtctggtag ctgccacggg gtagagagtg tggttgatca gcgtcgactt
     5581 gcccgagccg gaaacgccgg ttacgcaggt cagcaggccg cacggtatct cgaccgacac
     5641 ggactgaaga ttgttgccgg tggcgccgat gatgcggatg gtctcctcct cgacgaacgc
     5701 cgtccgcttt tcaggcacgg gaatcttctt cctaccggag agatactggc cggtgatgga
     5761 gcgcctggat ttcaggatct tctcgagact gccggcggca accacgcagc cgccgtgcac
     5821 gccggcccct ggtccgatgt cgatgatgtg gtctgcggag cggatcgcct cctcgtcgtg
     5881 ctctaccacc agcaccgtgt ttcccagatc gcgaaggtgc tccagagtct tgagcagccg
     5941 ggcgttgtcc cgctggtgca ggccgatgga aggctcgtcg aggatgtaca tgacgccgac
     6001 caggccggcg ccgatctggc ttgccaggcg gatgcgctgg gcttcccccc ccgaaagcgt
     6061 ctcggcgctg cggtccaggg tgagatagtt gagcccaacg tcgaccagaa accgcagacg
     6121 ggcgcgaatc tccttgagga tcttgtcggc gatctctccc cgtcgccccg acagcttgag
     6181 ctcgtcgaag taggccaggg agccctccac ggacccggac gttatctccg gcaggctgac
     6241 gccgtcgatg aagacgttgc gcgacgcctc gcgcagccgc gtgccgccgc agtcggggca
     6301 gctcctgacc cgaaggtact tctgcaggtt ctctcggacc aggctggagt cggtctcgtg
     6361 ataacggcgc tccatgttcg gtatcacgcc ctcaaaccgg tggcggcgat ggatcacgtc
     6421 gccgcggtcg ttggcgtagc tgaaatcgat ccgctctctg ccgctgccgt agaggattgc
     6481 gtcccgatgc tttttgggca gctttcggaa aggcgtctcc acgtcgaagc cgtagtgggc
     6541 tgcaagagag gagagcatgt ggaagtagta aacgttgcgc cggtcccagc cgcgtatggc
     6601 gccttcggcc agcgtcagct cctcgtcctg gaccaccagg ccgggatcga aaaactgctt
     6661 caccccaagg ccgtcgcaag cctggcaggc gccggcgggg ttgttgaagg agaacatgcg
     6721 gggctcgagc tcggatatgc tgtacccgca ggcgggacat gcgaaccttg ccgagaaaac
     6781 gagttccggc tcgtcggcat cgtccatgga cgccaccttc gcgatcccct cggcgagatc
     6841 tagagcggtt tcgaaggatt cagcgagccg ctgctgcgca tccgcccgga tacgcagccg
     6901 gtcgatgacg gcttccacgg tgtgcttttt gttcttctcc aggtttggcg ggtggtcgag
     6961 atcgaccacg atgccgtcga tccgggctct gatgaagccg tttgccatga gttcctgaaa
     7021 gacatgcagg tgctcgccct tacgttcggt gaccacgggc gcgaggacca ttacccgctt
     7081 gtcctccggc aggccgagaa cctggtccac catctggctg accgtctggg cgtcaagggg
     7141 caggtcgtgg ttggggcaac gcggctcgcc gaccctcgcg tacagcagcc gcagatagtc
     7201 atagatctcg gtgatcgtac ccacggtgga gcggggattg tgcgaggtgg acttctgctc
     7261 gatggagatg gccggggaca ggccctcgat atggtcgacg tccggcttct ccatcatcga
     7321 caggaactgg cgggcgtagg ccgagaggga ctccacgtag cggcgctgcc cctccgcata
     7381 gagggtgtcg aaggcaagcg aggactttcc ggatcccgat agcccagtga tcaccacgag
     7441 ccgatcgcgg ggtatgtcca gattgacgtt tttaagattg tgggtgcgtg ccccgcgaac
     7501 cgcgatgctg tccaatgcgc gtgccttatg ccctgccagc gcggctcgcc gaccctagct
     7561 tgcgctgatt gaccccggaa gtggggaaac ccggcaacat aaaccgatcc ctactcaggg
     7621 gcaagctgga ccatcgctta cggacttttc acggcgccgt gctagactgc gggcctggag
     7681 ttttctgggc atttgaacgg gtaagaggca acgggaaaaa ggctatggcg cggggtatca
     7741 acaaagtcat tctcatcggc aatctgggca gggatcctga gacgcgctac tcccagggcg
     7801 gcaatgcggt caccagcttc agcgtcgcca ccacggaaaa ctggcgggac cgccagtcgg
     7861 gcgagcagca ggaacgcact gagtggcaca acgtggtgtg cttcgcgcgc cttgccgaga
     7921 tcgcgggtga gtatctgcgc aagggctcga aggtttacat cgagggcagc cttcgtacca
     7981 gcagctggga gcaggacggt cagaaacgct accggactga ggtcatggcg agggacctgc
     8041 agatgctcga cagccgcggg ggcggtcagg gcggccaggg cggcgggttc gaatcagctg
     8101 cgcccgcagc gtccgagaac gcgccaacgc tggcggacga cgacttcgac gacgacattc
     8161 ccttctagtc ccggaatttg ttgaccagcc gggtcagctc ctggaggcgt tcccggggca
     8221 gctgcagctg cagcaggctc tgcttgatct cgggttctat gggaagcagc tcggccaggc
     8281 gggcgcccac cgaccgcgcg tcggcgaagt cgatttccaa cccgagcttc tgcaccccgg
     8341 gatgtttcac cagctctttg aggatgtcga ccagaggggc gtgctctgat ggcacggtgc
     8401 tatcgggctc ggccggcagg aagtccaccg ttccgagcag caggtggtcg gcctgctccc
     8461 gggtcgatac cacgcggaat ttgcggccac cccggacgat gatgccgagc cgtccgttcg
     8521 gcagctgatt gaagtcgacg atgtgggctt cggtgcctac ctcgaaaatc gcgggctggt
     8581 tggcgagcgg atccaggcga gcgtcgctgc ccgcaccccg tatgagcacg acgccgaagg
     8641 ggatcgactc ccgcgtgcag cggctcacca tgtccaggta gcgggtttcg aatatctgca
     8701 aaggcaaccg gccgccgtcg aaaagaacga cgcgcagggg gaacagcggg atctccatcg
     8761 gagaacctta gcacgctgct gcatcgcagt cgcgctgcgg tcgcgaaagg ggatgcttgc
     8821 cgttagactg cgcagccgtg gactggctgc agatcatcat ccttgccctg gtgcagggca
     8881 tcacggagtt cctgccgatc tccagctcgg ctcatctcat cctcccggca ttgctcaccg
     8941 agtggcccga tcaggggctg gcgttcgaca tagccgtgca tgcgggcacg ctgctggccg
     9001 tgctgtctta ctttcgccgc gagatcggcc gcttcgctgc cagtggtgcc cgtttgctgg
     9061 cccgcgggca gcgggatgac gacgccgacc tgcttctcaa gctgattgcc gcgacgttgc
     9121 ccatcgttct ggtgggctat ctcgcgcacg actgggtgga gtccagcctg cgcactgtgc
     9181 ccgtcatcgc ttctgccacc atcgtctttg cgcttgctct gtggtgggcg gacgggcgcc
     9241 ggagcggcgc cgatcatccg tcgtggacgg cggcaatgct catcggcgct gcgcaggtca
     9301 tcgccatcgt gcccggcacc tcccggtcgg gtattaccat gacggccgcg ctgctgctgg
     9361 gcttgagtcg tacgggtgcc gcccggttct cctttctgct ctccataccc acgattctgg
     9421 gcgcg
//
LOCUS       DBNHQR010000543         2428 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1322138, whole genome shotgun sequence.
ACCESSION   DBNHQR010000543 DBNHQR010000000
VERSION     DBNHQR010000543.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1322138"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="AC16VR_12410"
     CDS             complement(<1..186)
                     /locus_tag="AC16VR_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012004.1"
                     /translation="MESKTPLADLLAYRDGEELPADVARGIEGDPAAQGELEKLRGIR
                     QQLNALPAVLPDPAVWEE"
     gene            complement(143..739)
                     /locus_tag="AC16VR_12415"
     CDS             complement(143..739)
                     /locus_tag="AC16VR_12415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR6012005.1"
                     /translation="MASFAEIRLEPGVARGLKRGDRSAQGIAYRVLAPTVMGLACRIL
                     QDEGSAQEVVQDTFVDLIEHARTLKDAQAVVGWVRQVAVNHCLMRLRSPWNKRRDELD
                     ADPAGDVYVDMAEGAARTEGMQDIEKALSELEPETRLVIWLHDVEGYTHQEIAGLVGR
                     TASYSKSQMARGYQKLAKRFRGTEDGIENTASRSARVP"
     gene            complement(798..2075)
                     /locus_tag="AC16VR_12420"
     CDS             complement(798..2075)
                     /locus_tag="AC16VR_12420"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6012006.1"
                     /translation="MKFGIFYEHQLPRPWDDGAEQRLFNEALDQVELADRLGIDYAWE
                     VEHHFLEEYSHSSAPEVFLAACSQRTKNIRLGQGIRQVIPQYNHPARTAEVIATLDLV
                     SQGRVEFGTGESSAELELGGFRIPVAKKRKIFMEATEQICNMLAMDPYPGYKGQYFEM
                     PCRNVVPKPVQRPHPPVWVACSQRETIRMAARLGIGALTFAFVDPMEAQSWVDEYYGI
                     IKSDQCVPIGHAVNANICMVSSFSCHHDRQTAVQRGLEGFEFFGYALGSLYGFGEHKP
                     GRTDLWTEFQTVRAPQVRNEVADFSEALLGARGGIGTPEDLRQHLRKFERCGVDQVTF
                     IQQAGMNRHEHICESLEIFAAQVMGEFKEREAEREERKMRELEPYIAAAMERKVRMKP
                     LADDEIPTFPALGRRITEEQEDAPSIYERPAAG"
     gene            2222..>2428
                     /locus_tag="AC16VR_12425"
     CDS             2222..>2428
                     /locus_tag="AC16VR_12425"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6012007.1"
                     /translation="MTDRTFCYPRFVGNNDKPAANSRAELENRLARVFIEHGYEGASL
                     SRLATASGLGKASLYHHFPGGKAEM"
BASE COUNT          400 a          823 c          760 g          445 t
ORIGIN      
        1 ctcctcccac accgccggat cgggaagaac ggctgggagc gcgttgagct gctgccttat
       61 gccacgcagc ttctccagct cgccctgggc ggcagggtcg ccttcgatgc cgcgcgccac
      121 gtcggcgggc aactcctcgc cgtcacggta cgcgagcaga tcggctagcg gtgttttcga
      181 ttccatcttc cgttcctctg aatctcttcg cgagcttctg gtagcctcgc gccatctggg
      241 acttcgagta gctggcggtg cggcccacca gccctgcgat ctcctggtgg gtgtagccct
      301 ccacatcgtg gagccagatc accagccggg tctccggctc cagctcgctc agcgctttct
      361 ctatgtcctg catgccctcc gttcgggccg ccccttcggc catgtcgacg tatacgtccc
      421 ctgccgggtc cgcatcgagc tcgtcccgcc gcttgttcca gggggaacgc agccgcatca
      481 ggcagtggtt tacggcaacc tgccgcaccc agccgacgac cgcctgcgcg tccttgagcg
      541 tgcgcgcgtg ctcgatcaga tccacgaagg tgtcctgcac gacttcctga gccgatccct
      601 cgtcctggag aatgcggcag gcgaggccca tcacagtggg cgcgagcaca cggtaggcga
      661 tgccctgggc actgcggtca ccccgcttga ggccccgggc aacgcctggc tccaggcgaa
      721 tttcggcaaa actggccata ccactctata gatgcgtcag acccgcaaag ggtcgcagca
      781 acggagattt ttttcggcta cccggccgcc gggcgctcgt agatggacgg ggcgtcctcc
      841 tgttcctcgg tgatccgccg tccgagcgcc gggaaagtcg gtatctcgtc gtccgcgagc
      901 ggcttcatcc tcaccttgcg ctccatggcg gcagcgatgt aaggctcgag ctcacgcatc
      961 ttgcgctcct cccgctccgc ttcccgctcc ttgaactctc ccatgacctg cgcagcgaag
     1021 atctcgagag actcgcagat gtgctcgtgc cggttcattc cggcctgctg gatgaaggtc
     1081 acctgatcca cgccgcagcg ctcgaacttg cgcagatgct ggcgcagatc ctcaggggta
     1141 ccgataccgc ctctggcgcc cagcagagcc tcgctgaagt cggccacctc gttcctgacc
     1201 tgaggcgcgc gcacggtctg gaactccgtc cagagatccg tgcggcccgg tttgtgctcg
     1261 ccaaagccgt agaggctgcc aagggcgtag ccgaagaact cgaagccttc caggccgcgc
     1321 tgcacggcgg tttggcggtc gtggtgacag gagaagctgg acaccatgca gatgttggcg
     1381 ttgacggcgt gaccgatggg cacgcactga tcgctcttga tgatgccgta gtactcgtcg
     1441 acccaggact gagcctccat ggggtcgacg aaggcgaagg tcagcgcccc gatgcccagc
     1501 cgggcggcca tccggatggt ctcgcgctga gagcaggcca cccagacggg cggatggggc
     1561 cgctgcacgg gcttgggaac cacgttccgg cagggcatct cgaaatactg gcccttgtac
     1621 ccgggatacg ggtccatggc gagcatgttg cagatctgct cggtggcctc catgaagatc
     1681 ttgcgcttct tggccacggg aatccggaag ccgccgagct cgagctccgc cgaggactcg
     1741 ccagtgccga actccacgcg gccctgggag acgagatcca gggtggcgat cacctcggcc
     1801 gtgcgggccg gatgattgta ttgcgggatc acctgccgaa tgccctgacc caggcggatg
     1861 ttcttggtgc gctgggaaca cgccgccagg aagacctcgg gcgccgagga gtgggaatac
     1921 tcctcgagaa agtggtgctc gacctcccag gcatagtcga tgcccagcct gtccgcgagt
     1981 tccacctggt ccaacgcctc gttgaacagc cgctgctccg cgccgtcgtc ccagggccgc
     2041 ggcagctgat gctcgtagaa gatcccaaat ttcatgttca tgtccccact gtgttgtgca
     2101 gatccccggc ggccgggcgg ccagggcccc gaatggcgct ttcccgctgc ccgagtctcc
     2161 cctcaaaggg cacgtcgcat caaggggcac cgcgccagcg gcgcggatca gacggcgcct
     2221 tttgaccgac cgaacgttct gttaccctcg tttcgtgggc aacaacgaca agccagctgc
     2281 gaatagtcgc gccgagctcg aaaatcggct cgccagagtc ttcatcgagc acggctacga
     2341 gggcgccagc ttgagccgcc tggcgaccgc cagcgggctg gggaaagcca gcctctacca
     2401 tcactttccc ggcggcaagg cggagatg
//
LOCUS       DBNHQR010000544         7029 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1763684, whole genome shotgun sequence.
ACCESSION   DBNHQR010000544 DBNHQR010000000
VERSION     DBNHQR010000544.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7029)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7029)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7029
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1763684"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="AC16VR_12430"
     CDS             <1..666
                     /locus_tag="AC16VR_12430"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6012008.1"
                     /translation="DPNCRAIVLAAQGRHFCAGANFGSGKDDDSGSEDFTEEGFRNTT
                     GILYREAARLFGSKKPVIGAIQGAAIGGGLGLSLVPDFRVACPEARFGANFVKLGIHQ
                     GFGISVSLPRIIGQQAASMLLLTGRRIGGEEAFKLGLVDVLTDAESLRDEAMKLAQEI
                     AENAPLAVLSVRGTMRAGLAEAVIEATEHELKEQQWLRATEDAQEGIRSVAERRPGNF
                     QGR"
     gene            668..1540
                     /locus_tag="AC16VR_12435"
     CDS             668..1540
                     /locus_tag="AC16VR_12435"
                     /inference="COORDINATES: protein motif:HMM:TIGR03620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03620 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR6012009.1"
                     /translation="MKLGKLGVWYFLDGLPADAAAAAAQRIEELGYSALWIPEATGRN
                     PMVHASWLLANTKQLIVATGIANIYHREPGVTVSAQKALAEQSGDRFLLGIGVSHKPF
                     VEGVRGLTYGPPVASMRDYLDKMAAAPYTSVPPAEDPPTVIAALGPKMLALAAEKTAG
                     AHPYFVSPDHTAMASEVLGPDKWLCVEQKVILETDPSRARELARPAAQMYLALPNYRN
                     NWLRMGFTEADLGNGGSDRVIDATFAWGSAAQVEARIREHFDAGATHVCIQPVNPNGQ
                     FGDLHWEALEALAP"
     gene            1537..2220
                     /locus_tag="AC16VR_12440"
     CDS             1537..2220
                     /locus_tag="AC16VR_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6012010.1"
                     /translation="MSSDYTGNVVVVTGAAGALGRAVVEHFAARGATVAQLDVVAIDN
                     AHFSDTCDLTDPDSCRQAVTRIIETLGRIDVLANIAGGFTMGEAVHETSEETWDFMMG
                     LNARSVLNMARAVVPIMLQQGAGKIVNVGAGAGLAGVANMGAYSASKSVVIRLTEAMS
                     AELKERRINVNCVLPSIIDTERNRADMPDADFHRWVTPAAMAGVIGFLASPAADPIHG
                     AAVPVSGLS"
     gene            complement(2255..3997)
                     /locus_tag="AC16VR_12445"
     CDS             complement(2255..3997)
                     /locus_tag="AC16VR_12445"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR6012011.1"
                     /translation="MVNRRRFVEAGALTAAGLSLWGCGADTRPRSAGAGDGQVAGPGR
                     AVVFRSGVVLPVDAGFSEQRAIAIRGNRILAVGDDDDVLAAAGKDPQVVDLDGRVLLP
                     GFIEPHMHFALMAGLGHWPDIGPFKHESTEDALAALRLIARETPADEWIVARQFDPSL
                     QTGPEMLTTRELDTVSTDRPVFVLNASGHLAYGNSRLLEFAGIDRDTPDPPGGEYFRD
                     PDGRPNGAMSQVAYQPVMMRNEALIAVLGSGFVEAGLRVGEEAAALGITTLCDQATGG
                     IAGMGDIDFYRQMFASSRMSARIRASLYSETAAEWDDNGVAFGDGDALMRIVGWKIVT
                     DGSNQGFTGRQREPYLDSDNRGMYYVAPEALNEMVLSRGRRGWPLVLHGNGDAAIDSI
                     LDAVQAAADAGVDVGKLRCRIEHASILHDGQIQRIRELGMGPSFLINHVHYWGHAMRD
                     KVFGPEKVQLLDRCRSVEDAGITWTMHSDAPVSPLGTLHKIRVAVARDLWKEPDTTLA
                     PHERVSVEAAIRAVTINAAWQCHSETEVGSLAEGKLADLVVLEDDPRKVEPKAISEIG
                     VSQTWMDGNQVFGA"
     gene            4365..5369
                     /locus_tag="AC16VR_12450"
     CDS             4365..5369
                     /locus_tag="AC16VR_12450"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024151.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR6012012.1"
                     /translation="MEVWYPADDEHAGQDLDPDTQDHFQPMSLSPPVPQAAVRDAAPA
                     EGEPRPLLIFSHGFGGTGRQTTHLCTHWASHGYVVAAMDHVGNTTEDVMAAAAQPVDP
                     GTLLERLKSFAADRPVDVSFVIDRMVAGDAGLTVDPDRVGVSGHSFGGWTTLASAGMD
                     PRIRAALPLAPAGGQSSQWQSSIADRLADALAMSWEREVPTLFLVAEHDVVLPLDSMH
                     DLIERTPHPRRAVNLLNADHFHFCDRVEQTHDMVRMMGQMIFGTRSEADQEAPSALAA
                     MKPSSELCPGDHAYALLQGLGLAHMDAHVRELDRAHALLAGDLASLMAERGVAVTVMA
                     "
     gene            complement(5422..5901)
                     /locus_tag="AC16VR_12455"
     CDS             complement(5422..5901)
                     /locus_tag="AC16VR_12455"
                     /inference="COORDINATES: protein motif:HMM:NF024844.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR6012013.1"
                     /translation="MSDTETNMNNALEILRQRLDQPNPSSDWFEITQDRVNDYADVSM
                     DHQWIHVDVERAKQGPFGAPIAHGNLTIAVMGHLPRSETASQPPLEGHKLSINYGFDR
                     IRFPAPVRVGDRIRATSTPRRAEIKGGMIETMNEIVVEIEGQEKPALVAESLGRLVF"
     gene            complement(6010..>7029)
                     /locus_tag="AC16VR_12460"
     CDS             complement(6010..>7029)
                     /locus_tag="AC16VR_12460"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HIR6012014.1"
                     /translation="SNAGGLGVLGAAGCGPELLRRWIRETRELTDRPFGVDTLLPASV
                     RRANYDETEGPTPMDLLPETQAYAAEFMEREGLEPVASESPRPPRTSDEPAVFTKDFF
                     EAQMRVVIEERVPVYASGLGNPGPWMDELRANGTKVMAVVGAVRHAIQVKSSGLDIIV
                     AQGHDGGGHNSPIGTMALIPQVVDAVGDTPVLGAGGIVDGRGIAAAMMLGAEGVWIGS
                     AFLASQEANIFDFQKQAIVDATEEGTSVSRSVTGKPARIIRSKWTDYWARGERDPLPM
                     PFQGLVSQPVLAAANRDGRGDINPGFAGQGIGMIKAVRPAREIFDELVYGAEQALART
                     SNLLR"
BASE COUNT         1191 a         2428 c         2275 g         1135 t
ORIGIN      
        1 gatccgaact gcagggccat cgttctggca gcacagggcc ggcatttctg cgcgggggcc
       61 aatttcggca gcggaaagga cgacgactcc ggaagcgagg attttacaga ggagggcttt
      121 cgcaacacca cgggcattct ctaccgcgag gccgcgcggc tgttcggcag caagaaaccg
      181 gtaatcggcg cgatccaagg cgccgccatc ggcggaggcc tgggtctgtc cctggtcccg
      241 gattttcgcg tcgcctgtcc ggaggcgcga ttcggagcca acttcgtcaa gctcggcatt
      301 caccagggct tcggcatctc cgtcagcctg ccccgcatca tcggtcagca ggctgcaagc
      361 atgctgctgc tgacaggccg gcgcatcggc ggcgaagagg cgttcaagct cggcctggtg
      421 gacgtactga ccgacgccga gagcctgcgc gacgaggcga tgaagctggc gcaggaaatc
      481 gccgagaacg ccccgctggc cgtcctgtca gtcaggggca ccatgcgcgc cgggctcgct
      541 gaggccgtca tcgaggccac cgagcacgag ctcaaggagc agcaatggct gcgcgccacc
      601 gaggacgccc aggaaggcat ccgctccgtg gccgagcgtc ggcccggcaa cttccagggc
      661 cgttgaggtg aagctcggca agctcggcgt ctggtacttt ctcgacggcc tgccggcgga
      721 tgccgcggcc gccgccgctc agcgcatcga ggaactgggt tacagcgctc tgtggatacc
      781 ggaggcgacg gggcgcaacc ccatggttca cgcctcgtgg ctgctggcca acaccaagca
      841 gctgatcgtg gccacgggaa tcgccaacat ctaccaccgg gaacccggcg tcacggtctc
      901 ggcgcaaaag gccctggccg agcagtcggg cgaccggttc ctgctcggaa ttggtgtttc
      961 tcacaagccc ttcgtggagg gcgtgcgcgg cctgacttac ggcccgcccg tggcgagcat
     1021 gcgggactat ctggacaaga tggccgctgc gccctacacg agcgttccgc cggccgaaga
     1081 cccgcccacg gtgatcgccg cgctgggccc gaagatgctg gccctggccg ccgagaagac
     1141 tgcaggcgca cacccctact tcgtttcgcc cgaccacacc gccatggcca gcgaagtcct
     1201 cggtccggac aagtggctgt gcgtagagca gaaagtcatt ctggagacgg atccttcccg
     1261 ggcccgggag ctcgcccgcc cggcggcgca gatgtacctg gccctgccca actaccgaaa
     1321 caactggttg cgcatgggct tcaccgaagc cgacctcggc aacggcggtt ccgatcgggt
     1381 catcgacgcc acctttgcct ggggcagcgc ggcccaggtt gaagcccgca ttcgcgagca
     1441 ttttgatgcc ggcgcgaccc acgtctgcat ccagcccgtc aatcccaacg gtcagtttgg
     1501 cgatctacac tgggaagccc tggaggcgct cgcgccatga gcagcgacta tacaggcaac
     1561 gtagtcgtcg tcacgggcgc ggccggcgcc ctgggccgtg ccgtggtcga gcacttcgcc
     1621 gcacgcggcg ccaccgtggc gcagctggac gtggtggcca tcgacaacgc ccacttctcc
     1681 gacacctgtg atctgaccga cccggacagc tgcaggcagg cggtcacccg gataatcgag
     1741 acgctggggc gcatcgacgt gctggccaac atcgccggcg gcttcaccat gggagaggcc
     1801 gttcacgaaa cctccgagga gacctgggat ttcatgatgg gcctgaacgc gcgctcggtg
     1861 ctgaacatgg cccgggccgt ggtccccatc atgcttcagc agggggccgg caagatcgtc
     1921 aacgtcggcg ccggcgcagg gctcgccgga gtcgcaaaca tgggtgccta cagcgcgtct
     1981 aagagcgtgg tcatccgatt gacggaagcc atgtccgcgg agctcaagga gcgacgcatc
     2041 aacgtcaact gcgtgctgcc gtccatcatc gataccgagc gcaaccgggc cgacatgccc
     2101 gacgccgatt tccaccgttg ggtgacgccc gcggccatgg ccggggtcat cggctttctg
     2161 gcgtcacctg cagcggaccc cattcacggc gccgccgtgc ccgtcagcgg gctgagctga
     2221 ggggcgcggg ccgaccggcc cgcgacgctg cggatcaggc tccaaatacc tggttcccgt
     2281 ccatccacgt ctggctcacc ccgatctcgg agatggcctt gggctcgacc ttcctcgggt
     2341 cgtcctccag cacgaccaga tcggccagct tgccttccgc taggctgccg acctcggtct
     2401 ccgagtgaca ctgccatgcc gcgttgatgg tgacggccct tatcgccgcc tccacggaaa
     2461 cgcgctcgtg gggcgcaagt gtggtgtcgg gctccttcca gaggtcgcgg gccaccgcaa
     2521 cccggatctt gtgcagggtg ccaagcggtg acaccggcgc gtcgctgtgc atcgtccaag
     2581 tgatgccggc atcttcgacg ctccggcagc gatcgagcag ctgcactttc tccgggccga
     2641 acaccttgtc gcgcatggcg tgaccccaat agtgcacgtg gttgatgaga aagctggggc
     2701 ccatgcccag ctcgcgaatg cgctggatct gcccatcgtg gaggatcgac gcgtgctcga
     2761 tgcggcagcg cagctttccc acgtccacgc ccgcatccgc cgccgcctgc accgcgtcca
     2821 gaatgctgtc gatcgcggcg tcgccgttgc cgtgcagcac cagcggccag cctcgacggc
     2881 cacggctgag caccatttca ttcagcgctt cgggcgcgac gtagtacatg ccccgattgt
     2941 ccgagtctag atacggctca cgctgccggc ccgtgaaacc ctggttggac ccatcggtca
     3001 cgatcttcca gcccacaatg cgcatcaggg cgtccccgtc accgaaggca acgccgttgt
     3061 cgtcccactc cgcggccgtc tcggaataga gcgaggcgcg aatccgcgcg ctcatccggc
     3121 tcgaggcaaa catctgccgg tagaagtcga tatcgcccat gccggcaatg cccccggtgg
     3181 cctggtcaca gagggtggtg atcccgagcg cggccgcctc ttcgccgacc cgcaacccgg
     3241 cctcaacgaa cccggagcca agaaccgcga tgagcgcctc gttccgcatc atcaccggct
     3301 ggtaggcgac ctgggacatg gcgccgttgg gccgcccatc ggggtcacgg aaatactccc
     3361 cgccgggcgg atccggcgtg tccctgtcga tgccagcgaa ctccagcagg cgggagttac
     3421 cgtaggcgag atggcccgag gcgttcagca caaagacggg ccggtctgtt gacacggtat
     3481 ctagctcccg ggtcgtcagc atctcgggac ccgtctgaag gctgggatcg aactgacggg
     3541 caacgatcca ctcatcagcc ggcgtctccc tggcaatgag gcgcagagcg gcgagcgcgt
     3601 cctcggtgct ttcgtgtttg aacgggccga tgtcaggcca gtggcccagc ccggccatca
     3661 gcgcgaagtg catgtggggt tcgatgaatc ccggcaggag gacccggcca tcgagatcga
     3721 ccacctgggg gtctttcccc gccgccgcca gcacgtcgtc gtcatccccg acggcaagga
     3781 tgcggtttcc gcggatggca atcgccctct gctcggagaa tcccgcatcc accggcaaca
     3841 ccacgccgct gcggaaaacg acggcgcggc cgggacccgc aacctgcccg tcgccggccc
     3901 cggcagacct cggacgggta tcagcaccgc aaccccacag cgacaagccg gcagccgtca
     3961 gggcgccggc ctctacgaat cgtcttctgt taaccatccc ggcgggcctc caaaatctgt
     4021 tcttccctcg acttccggtg agtcctgccg ccaacgggcc acggtctcct ttgctgcgtc
     4081 agcgtacacg gcgagccagc cgaaacgaag caacccgata catgcaaggc tcatgctggc
     4141 ggcaatggaa tcctctcggt atccttcgcg gcgcgtcgag atctcgagag tcgagccagc
     4201 ctcgattcgg gcagcaccac gctgccagac tgcgagcccg acgctcaacc gttctgacac
     4261 agaggtagac gatgacatcg tatgatccgt tcctgcgtgg cccgcatccg gtcggcgtgc
     4321 gcacctttca ccggaccgac aaaacaaggc aacgaacgtt acccgtggaa gtgtggtacc
     4381 cggccgacga cgagcacgcc gggcaggatc tcgacccgga tacccaggat cacttccagc
     4441 ccatgagcct gtcgccaccg gtaccccagg cagccgttcg cgacgcggca ccggccgagg
     4501 gtgagccccg gcctctgctg atcttctctc atggcttcgg cgggaccggc cgccagacga
     4561 cacacctgtg cacccattgg gccagtcacg gctacgtggt cgccgccatg gaccacgtcg
     4621 gcaacaccac ggaggacgtg atggcggcgg ccgcccagcc agtggatcca gggaccctgc
     4681 tcgagcgcct gaaatcgttt gccgcagacc ggccagtgga cgtcagcttc gtgatcgacc
     4741 ggatggtggc cggcgatgcc ggcctgaccg tcgatcccga ccgcgttggc gtctccgggc
     4801 acagcttcgg cggctggacc accctggcat cggccggaat ggatccacgc atccgcgccg
     4861 ccttgcccct ggcaccggcc gggggccaat cgagccagtg gcagtcgagc atcgccgacc
     4921 gcctggcaga cgcgctcgcc atgagctggg agcgcgaggt acccacgctg tttctggtcg
     4981 ccgagcacga cgtggtcctg ccgctggaca gcatgcacga cctcatcgag cgaacgccgc
     5041 acccgcgccg cgccgtgaat ctactgaacg ccgatcactt ccacttctgc gatcgggtgg
     5101 agcagaccca cgacatggtg aggatgatgg gacagatgat cttcggcacg cgcagcgaag
     5161 cggaccagga agcgccctcc gcgctggcgg ccatgaagcc gagcagcgag ctgtgccctg
     5221 gcgatcacgc ctacgcgctg cttcaggggc tgggcctggc ccatatggac gcccacgtga
     5281 gggagctcga ccgggcccac gcgctgctgg cgggcgacct ggcgtcgctg atggccgaac
     5341 ggggcgtggc ggtcacggtc atggcctgag cagcgcacgc cggcaccccc cgttcccccg
     5401 tccgggtgac ggcccggcag atcagaagac gaggcgaccc aggctttcgg ccaccagcgc
     5461 cggcttttcc tgaccctcga tctcgaccac gatctcgttc atggtctcga tcatgccgcc
     5521 cttgatctcg gcgcggcgcg gcgtgctggt ggcgcggatc ctgtcgccca cccgcacggg
     5581 cgcgggaaaa cggatacggt cgaagccgta gttgatgctg agcttgtggc cctccagcgg
     5641 tggctgggag gccgtctcag atcgcggcag gtggcccatc acggcgatgg tcaggttgcc
     5701 gtgggcgatg ggcgcaccga acggcccctg cttggcccgc tcgacgtcga cgtggatcca
     5761 ctggtgatcc atgctgacgt cggcatagtc gttgacccga tcctgagtga tctcgaacca
     5821 gtctgaggag gggttaggct ggtcgagacg ctgtcgcaga atctccaggg cgttgttcat
     5881 gttggtttcg gtgtcggaca tgatctcgtt ctccggtaat cgtcaaaaat caaacgggcg
     5941 gggcccaccg gctggcatcg tcgttcgcat cgagggagcc gatgaggtcc acgatcgggc
     6001 cggcgccgct cagcgaagca ggttcgaagt tcgagcgagc gcctgctcgg ccccgtaaac
     6061 cagctcgtcg aatatttccc gggccgggcg gacggccttt atcatgccga tgccctggcc
     6121 ggcgaaaccc gggttgatgt cgccccggcc atcgcggttc gccgcggcca gcaccggctg
     6181 ggagaccaag ccctggaagg gcatcggcag gggatcccgc tcgccccgcg cccagtaatc
     6241 ggtccatttg ctgcgaatga tccgtgccgg cttacccgtc acggaccggg acacggaggt
     6301 tccctcctcg gtggcatcga cgatggcctg cttctgaaaa tcgaagatgt tggcttcctg
     6361 ggacgcgaga aacgcggagc cgatccagac cccctcggct ccgagcatca tggctgcagc
     6421 aatgcctcgg ccgtcgacga tgccaccggc gcccagcacc ggcgtatcgc ccaccgcgtc
     6481 caccacctgg ggtatcagcg ccatggtgcc gatgggcgaa ttgtgaccgc cgccgtcgtg
     6541 accctgggcc acgatgatgt cgagcccgga ggacttcacc tgaatcgcgt gccggacggc
     6601 gcccaccacg gccatcacct tggtgccgtt ggcgcgcagc tcgtccatcc acggtccggg
     6661 gttgcccaga ccggacgcgt aaacggggac ccgctcctcg atgaccacgc gcatctgcgc
     6721 ctcgaagaaa tctttcgtga acacggcggg ctcgtcggac gttcgcggcg gccgcggcga
     6781 ctcgctcgcc accggctcca ggccttcgcg ttccatgaac tcagccgcgt aggcctgggt
     6841 ctcaggcagc agatccatgg gcgtcggccc ctcggtctcg tcgtagttgg cgcgccgaac
     6901 cgacgcaggc agcagcgtgt cgacaccgaa gggtctgtcg gtgagctcgc gcgtctcgcg
     6961 gatccagcgc cgcaggagct ccggaccaca tcccgcggct cccagcacac cgagcccgcc
     7021 ggcgttgga
//
LOCUS       DBNHQR010000545         4421 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_940526, whole genome shotgun sequence.
ACCESSION   DBNHQR010000545 DBNHQR010000000
VERSION     DBNHQR010000545.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4421)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4421
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_940526"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1465
                     /locus_tag="AC16VR_12465"
     CDS             <1..1465
                     /locus_tag="AC16VR_12465"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR6012015.1"
                     /translation="NLEFDAPNTPQTRFNAGSVAKQFTATAILRLEQSGELSLDDPIR
                     RYVPELPGVARSITLRQMLHHTSGLRDVWALTDLGGWQPADVRTPTQALRILARQRAL
                     NFLPGNGFGYSNSGYLLLAEVIRRTTGRPFPDWMRQHVLEPAGMEDTYFYEDPIKLLP
                     GVASPYRFLGRSRGFARDDLNSGLAGSGNLVTTAADLGRWSAHLLTSKIGGAPLVERL
                     SEQGILPDGRKTGYGMGLFVGTHRGLPVVSHGGANAGYRSELLMFPDQRVAVVVLGNV
                     NTVSAERLARSMADVVLAEDLPAPSPLPETTPVPMALPPASFAGRYALGQMLLEIRES
                     DGSLYFRIGGGDLRPLTATGPVTFATSEPGVSLEFQLDENRSITGVDLLAAGRTLPAE
                     RLEPEMLTATDARAYEGRYHSEELDTSYRVVYENGHLVARHLRGSDIVLTAFGQDRFL
                     ESAGGDLRVDFQRSRRGRITGFEVSVDRARAIAFERI"
     gene            complement(1514..2884)
                     /locus_tag="AC16VR_12470"
     CDS             complement(1514..2884)
                     /locus_tag="AC16VR_12470"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spinster family MFS transporter"
                     /protein_id="HIR6012016.1"
                     /translation="MGGPSAGSGAALQRPGTAAAWYATVILTLAYTFSFVDRQVLNLL
                     VEPIRHDLGLSDTSISFLQGLAFVIPYVLMSVPIGRLVDKFNRILVLIAGVLFWSVAT
                     FGCGVAGNYGQLMAARMGVGVGEASLTPAAWSLLADYFPPDKLARPVSFFLMGPYLGA
                     GLALIGGAAVIDWAASIGELSLPVLGSIRAWQFTFMMVALPGVLIAALLAMLPNPQRQ
                     REAGDAEPAPGWSEVMRFMKRHARIYLAVLLGVPFLVVVLYGLQGWVPTILVRVYGWD
                     LASAGRIYGTLALVAGSAGVLSGPVVGGWLKRRGYADYPLRLATGAAAAVALSMVTLP
                     FQTSGAGALVCIGLASFFVTLPLALMTFVTQTVTPRNMRGVLAGFFVVGTNVIGLGLG
                     PTLVAATTDYVLGDPGKVHVSLMIISVCVAPVAMLLLWSGMRALGEWERQNQGSPDVE
                     DSTAGR"
     gene            complement(2918..3268)
                     /locus_tag="AC16VR_12475"
     CDS             complement(2918..3268)
                     /locus_tag="AC16VR_12475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HIR6012017.1"
                     /translation="MIIVVARLVFDTQEHRDKAVELSTDIQGRTRDEEPGCRMYCFAP
                     DPCVPAEIQVYELWDDAASLEAHFKHPNYAAMVEALGSAGGLLESINRQYVADDRGTV
                     YGDDRTPRIQALLD"
     gene            3405..4121
                     /locus_tag="AC16VR_12480"
     CDS             3405..4121
                     /locus_tag="AC16VR_12480"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6012018.1"
                     /translation="MRTQEGSDARDLGELSDIEAVSRLLDVKDLRLIDVGCGAGHLAR
                     SLAELGAIVLGIEPDPAQAERNRTADPVPNVTLLEAGAEKIPADDHSVDGVMFFRSLH
                     HVPRQLMDASLIEAARVLKPEGFLCVVEPGMEGSHYAMMRPFHDETEVRTLAQEALAR
                     TAEDLFDSAHGYLYTQHPRHDSFEAMVEFFTSLSFNDISRGMIDVPEVHKHFEAARTD
                     EGYVFEQPTLVNVYRHPKAN"
BASE COUNT          842 a         1494 c         1359 g          726 t
ORIGIN      
        1 gaacctggag ttcgacgcgc ccaacacccc gcaaacccgt ttcaacgcgg ggtccgttgc
       61 caagcagttc accgccacgg ccatcctcag gctcgagcaa tctggggagc tcagcctgga
      121 tgacccgata cgtcgatacg tgcccgagct gcctggggtt gccaggtcca ttaccctgcg
      181 gcaaatgctt catcacacca gcggtctgcg ggacgtctgg gcgctcacgg acttaggcgg
      241 ctggcagcca gccgacgtga ggacgccgac ccaggctctg cgtatcctgg cgcgccagcg
      301 ggcgctgaat tttttgcccg gcaatgggtt cggctactcc aacagcggct atctcctcct
      361 ggccgaagtg atccgacgta ccaccggcag gccgtttcca gactggatgc gccagcacgt
      421 cttggaaccg gccggcatgg aggataccta cttctacgag gacccgatca agctgctgcc
      481 aggcgtcgcc agcccgtacc gcttcctcgg cagaagcagg ggttttgctc gtgacgatct
      541 caacagcggg ctggcaggca gcggcaatct cgtcacgacg gcagccgacc tcgggcgatg
      601 gtcggcgcat ctcctgacat cgaaaatcgg gggcgctccg ctcgtcgaac ggctctccga
      661 gcagggcatc ttgccggacg gtcggaagac cgggtacggc atgggcctgt tcgtcgggac
      721 ccaccgcggc cttcccgtgg tctcgcacgg aggcgccaac gctggatacc gaagtgagtt
      781 gctgatgttc cccgatcagc gggttgccgt cgtggttctg ggcaacgtca acacggtctc
      841 ggcggagcgg ctggcgcgat ccatggcgga cgtcgtcctc gcagaagacc tgcccgcccc
      901 ttccccgctt ccggaaacca cacccgtgcc catggccctg ccgcccgcaa gctttgccgg
      961 ccgctacgcc ctgggccaga tgctgctgga gatccgcgag tccgacgggt ccctttattt
     1021 ccgcattggc ggcggcgacc tgcgcccgct gaccgccacc ggaccggtga ctttcgccac
     1081 ctcggaaccg ggcgtctcgc tggaatttca gcttgacgag aacagatcaa ttaccggcgt
     1141 cgatctgctc gctgccggac gcacgctccc ggccgagcgc ctcgagccgg aaatgcttac
     1201 cgccacagac gctcgggctt acgagggccg ctaccacagc gaggaactcg acacctcata
     1261 ccgtgtcgtt tacgagaacg gccacttggt cgccagacac ctgcgcggga gcgatatcgt
     1321 gctcacggcc tttggtcagg accgtttcct ggaatcagca gggggtgatc tgagggtcga
     1381 ctttcagcgc tcccggcgcg gtcgaatcac cggcttcgaa gtgtccgtcg atcgggcccg
     1441 ggccatcgcc ttcgagcgga tctgaaaccc caggagtgat cagacgacgg cccggaaacc
     1501 gacccagcca ccctcatctc ccagccgtcg aatcttccac gtcgggcgat ccctgattct
     1561 gccgctccca ctccccaagc gcccgcatgc cgctccacag gagcaacatg gcaacggggg
     1621 ccacgcagac cgagatgatc atcagcgata cgtgcacttt gccgggatcg ccgagaacat
     1681 agtccgttgt cgccgcgaca agcgtcgggc caagccccag cccgatgacg ttcgtcccga
     1741 cgacgaagaa gcccgccagg acgccacgca tgttgcgggg ggtcacggtc tgcgtgacga
     1801 aggtcatcag cgccagcggc agcgtgacga aaaagctggc aagcccgatg cagacaagcg
     1861 cgcccgcgcc cgacgtctgg aacggcaggg tcaccatgct cagcgcgacg gccgccgccg
     1921 cgccggtggc cagacgcagc ggatagtcgg catatccccg ccgcttcagc cagccgccca
     1981 cgacgggacc gctgagcacg cccgccgagc ccgccaccag cgccagggtt ccgtagattc
     2041 gtccggcgct ggcgaggtcc cagccgtata cgcgaacgag gatcgtcggt acccaaccct
     2101 gcagcccgta gaggacgacc acgagaaagg ggacacccag cagcacggca aggtagatac
     2161 gggcgtggcg tttcatgaat cgcatgactt cgctccagcc cggtgccggc tcggcatcgc
     2221 cggcttcccg ctgacgctgg ggattgggga gcatggccag cagcgccgcg atcagaacgc
     2281 ccggcaacgc caccatcatg aaggtgaact gccacgcgcg gatgcttccg agcacgggca
     2341 acgacagctc accaatggag gccgcccagt cgatcacggc ggctccaccg atcagcgcaa
     2401 ggcccgcgcc cagatacggt cccatgagaa agaagctcac gggacgcgcc agcttgtccg
     2461 gaggaaagta gtcggcaagg agggaccagg ccgccggggt cagagaagcc tcccctaccc
     2521 ccacacccat tctcgcggcc attagctgtc cgtagtttcc cgccactccg cagccgaagg
     2581 tcgccacgct ccagaacagc acgcccgcta tcagcaccag gatgcggttg aacttgtcca
     2641 ccaggcgccc gatgggcacg ctcatcagca cgtaggggat gacgaaggcg agcccctgca
     2701 gaaagctgat gctggtgtcg ctgagcccca gatcgtgcct gatcggctcg accaggaggt
     2761 tgagaacctg ccggtcaacg aaagagaacg tgtaggccag cgtcaggatg acggtcgcgt
     2821 accacgcggc cgccgtcccc ggtcgctgca gagcggcgcc ggagcctgcc gaaggcccac
     2881 ccaccgctat ggaatcctct gcgttaccgc gggcacctca atccagcaga gcctggatac
     2941 gcggcgtgcg atcatcgccg tagacggtgc cgcgatcgtc tgctacgtat tggcggttga
     3001 tggactcgag aagaccccca gcgctgccca gggcttccac catggccgcg taattagggt
     3061 gcttgaagtg cgcttccagc gacgcggcat catcccaaag ctcgtacacc tgtatctcgg
     3121 ccggcacgca gggatccggc gcgaagcagt acatgcggca tccaggctcc tcgtcccgag
     3181 tccgaccctg gatgtcggtg gacagctcca ccgccttgtc gcggtgttcc tgagtgtcga
     3241 aaacgagccg ggcaactacg atgatcatgt ctttctcctg atcttatgtg gcaccgaaga
     3301 gcagccaaga acgggcgatt ccgtgcggcg ccatccgcag caacgtctac cgcggatgaa
     3361 acggcgtgag gccctccctt cgccctcatt aaaccggcgc aaaattgaga actcaagaag
     3421 gttccgacgc ccgcgatctc ggtgagctat cggacatcga agccgtatca cgattgctgg
     3481 acgtcaaaga tctccgtctg atcgacgtcg ggtgcggcgc cggccacctc gctcgctccc
     3541 ttgccgagct gggcgccatc gtcctgggca tcgagcccga tccggcacag gcggagcgta
     3601 atcggaccgc ggatcctgtc ccgaacgtga cgctgctcga ggcgggggcc gagaagatcc
     3661 ccgcggacga ccacagcgtc gacggcgtca tgttcttccg ctcgctgcac cacgtgccgc
     3721 gccagctgat ggacgcgtcg ctcatcgagg cagccagggt gctcaagccc gaaggattcc
     3781 tctgcgtcgt ggagcccggc atggagggga gtcactacgc gatgatgcgg ccgttccacg
     3841 acgaaacgga agtgcgcacc ctcgcccagg aggccctggc ccgaacggct gaggacctgt
     3901 tcgatagcgc ccacggctac ctctacaccc agcacccgcg ccacgacagt ttcgaggcca
     3961 tggtggagtt cttcaccagc ctgtcgttca acgacattag ccggggaatg atcgacgtcc
     4021 ccgaagtgca caagcatttc gaggcggcgc gtacagacga aggctatgtc ttcgagcagc
     4081 ccacgctggt gaacgtctac cgccacccga aggccaactg accggccaat cggcccgtcc
     4141 gagcgttctt tttcatcggg aatcggacat tctgaggact cgttcccatt ggttggcccg
     4201 ccatatgagg cgatgttctc atcctggagc cacttcgctg gatacactcg atgcaaccgt
     4261 caaaggggtc gagcatgaaa acattcatgg acctatccgc agttctgttg ctatcgacct
     4321 gcgtggcggg cgagcaacat cgcgatgacg tgcccatcat ggccggcgtc gttgatacgc
     4381 gctcgggtga catccgaagg tgcgagtgga aaggctacga c
//
LOCUS       DBNHQR010000546         3764 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_687941, whole genome shotgun sequence.
ACCESSION   DBNHQR010000546 DBNHQR010000000
VERSION     DBNHQR010000546.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_687941"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..2175)
                     /locus_tag="AC16VR_12485"
     CDS             complement(133..2175)
                     /locus_tag="AC16VR_12485"
                     /inference="COORDINATES: protein motif:HMM:NF013199.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR6012019.1"
                     /translation="MARLTHLPTDLDGGERVRGFAFFIAGLLTIWGASGCSAPEDARD
                     EAPIEGPTAAAPTETGGASDEAGEGAVLASTDEFQVGLGDLGDREALPGAALYADNCA
                     ACHDGSVPKAPLFTWLEMMSARTLLGAMNDGIMAAQAADLTADERVHIVEYLTRQRYV
                     AGAVDDLADAHCEAPRAAFDRDRPAPRVGWGHDTRRFVPGDVAGLSAADVPDLELAWA
                     FVFPDALRARSQPAIGYGAVFVGSQDGRVYALDLETGCVRWHYQAAAEVRTGIVVAEI
                     HGRPMAFFGDILAWVHAVDALTGEPVWKRKADEHPGATLSGTPALHEGVLYVPVSSLE
                     IIAAADPAYECCTFRGSVLAVNAANGSERWRSYSIPEPPTVAQKTSAGTNVYAPSGAP
                     IWASPAIDAKRNRLYVGTGENYSTPADGNSDAVLAIDLTTGERIWTRQMTEGDAWNVA
                     CMMADNPNCPPEDGPDFDLGSSQMIVDLADGRQVLVGGHKIGAAFAFDLDAVKGDDPE
                     PLWWTRVGRGSIQGGVHFGMAADGTTIYVPINDMNDTRNGDVLDPEAARPGVHAVNAA
                     DGKVLWSRVQPDRCGDDRPFCDPGISAATTAVPGVLFAGHLDGVIRAYERDTGNVLWS
                     FDSAREFTGVDGRVGRGGGMSGAGPAVGEGYVVVNSGYGLYFHEPGNVLLAFRPSR"
     gene            2233..>3764
                     /locus_tag="AC16VR_12490"
     CDS             2233..>3764
                     /locus_tag="AC16VR_12490"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-containing oxidoreductase family
                     protein"
                     /protein_id="HIR6012020.1"
                     /translation="MSEVRTAICRFCHSFCGVNVTLDDGRIIKVLGDIENPMYHGYSC
                     IKGRQLAEQHYHLERLLAPHKRSNGSHGKVPSMQALDEIAERLGELIDAHGPRSVATY
                     TGTYSFPYPAGTAMATAFMDAIGSPMRFTSGSIDQPGKPMAIAFHGRWSAGPPAFADS
                     DVWMLVGANPVVSKWGGIPQYNPGKRLHDALKRGMKLIVIDPRETECAKRAHLHLQCK
                     PGEDPTILAGIMHVIINEGLHDAEFIDSDVTGFDALKDAVADFTPDYVAQRAEIPKAQ
                     LIEAARTFAAASRGMTTAGTGPNMAPRGTLTEYLVLSLNTLCGRWLQAGEVMPNPFAL
                     LPERRGRAQPEPKPEPFGYGERLRVRGLTENVSGLSAAALADEILLEGEGQVKALFCV
                     GGNPMAAWPDQLKTYQAMKALELNVCLDIKMSATAKLAHFVLPPRLGLESPAISLPNE
                     NIWFYGFSTGFPEPYAMYQPQLVEPPAGSDLLEEWEVFYGVAQRMGLGLTFAGEGLDM
                     NR"
BASE COUNT          660 a         1338 c         1210 g          556 t
ORIGIN      
        1 acccggatag tgctcgaaat agattcgtct atcgccttct acggccagat gtgccacgac
       61 ttcccccatc aaaaaaccaa ggctcatcga gcctaaggat cctcggcatt cagcgcaacc
      121 ggggctgccc gatcagcggg acggtcgaaa ggccagcagc acgttgccgg gctcgtggaa
      181 gtaaaggccg tagcccgaat tcaccaccac gtagccctcg cccaccgcag gccctgcccc
      241 gctcatgccc ccgccgcgac caacccgccc gtccaccccg gtgaactccc gggcggaatc
      301 gaagctccac aagacgttcc cggtgtcccg ctcgtaggca cgaatgacgc cgtcgagatg
      361 gcccgcgaag aggacgcccg ggaccgccgt ggtggccgcc gagatgcccg ggtcgcagaa
      421 cggccgatcg tccccgcagc ggtcgggctg cacccggctc cacagcacct tgccgtccgc
      481 cgcgttgacc gcgtggacgc cgggccgggc cgcctcggga tcaaggacgt cgccgttgcg
      541 ggtatcgttc atgtcgttga tgggcacgta gatggtggtg ccgtcagcgg ccatgccgaa
      601 gtgcacgccg ccctgaatgc tgccccgccc cacccgcgtc caccacagcg gctccggatc
      661 gtcacccttc accgcgtcca ggtcgaaggc gaaggcggcg ccgatcttgt ggcccccgac
      721 cagcacctgc cggccgtcag ccaggtcgac gatcatctgg ctggagccga gatcgaaatc
      781 cgggccgtcc tccggcgggc agttcgggtt gtcggccatc atgcaggcca cgttccaggc
      841 atcgccttcg gtcatctgcc gcgtccagat gcgctcaccg gtggtgagat cgatggcgag
      901 cacggcatcg ctgttgccgt ccgccggggt ggaatagttc tcgccggtgc ccacgtagag
      961 ccggttgcgc ttggcgtcga tggccggact ggcccagatg ggcgcgcccg aaggggcgta
     1021 gacgttggtg cccgcagacg tcttctgcgc gacggtcggg ggctcgggga tggagtagct
     1081 gcgccagcgc tcggagccgt tggccgcgtt cacggcgagc acgctgccac ggaacgtgca
     1141 gcactcgtag gcggggtcgg cggccgcgat gatctccagc gaggagacgg gaacgtacag
     1201 gacgccctcg tgcagcgcag gggtacccga cagcgtggct ccggggtgct cgtccgcctt
     1261 gcgtttccag accggctcgc ccgtgagcgc atccaccgcg tgaacccacg cgaggatgtc
     1321 gccgaagaag gccatgggcc gaccgtgaat ctccgcgacg acgatccccg ttcggacctc
     1381 ggccgccgcc tggtagtgcc agcgcacgca gcccgtttcc agatccaggg cgtaaacccg
     1441 gccgtcctgg ctgccgacga agacggcgcc gtagccgatt gccggctggg aacgcgcgcg
     1501 cagcgcgtcg ggaaagacga aggcccaggc gagctccaga tcgggcacgt cggccgcgct
     1561 caggccggcg acgtcaccgg gaacgaagcg acgcgtgtcg tgcccccagc caacccttgg
     1621 cgccggacga tcacggtcga aggcggcgcg aggcgcttca cagtgggcgt ccgcaagatc
     1681 gtcgacagca cccgccacgt agcgctgacg ggtcaggtac tcgacgatgt ggacgcgctc
     1741 gtcggccgtc agatcagcgg cctgggcagc catgatcccg tcgttcatcg ctccgagcag
     1801 cgtgcgcgcg gacatcatct ccagccaggt gaacaggggc gccttgggca cgctgccgtc
     1861 gtgacaggcc gcgcagttgt cggcgtaaag ggccgcgccc ggcagcgcct cgcggtcacc
     1921 cagatcgccg agccccacct ggaactcgtc ggtagacgcc agaacggccc cctcgccggc
     1981 ttcgtccgag gcacccccgg tttcagtcgg cgccgccgcg gtcggaccct cgatgggcgc
     2041 ctcatcccgg gcatcctccg gcgccgagca cccggaagca ccccagatcg tcagcagccc
     2101 tgcaatgaaa aaggcaaacc cgcgcacccg ctccccccca tccagatcag ttggcagatg
     2161 tgtgagccta gccatttgcc ggcccaaccg caaagcgaca aaatgggccg ggccaatcgg
     2221 ggagaaatca tcatgtcgga agtgcgcaca gccatctgca ggttctgcca ctcgttctgc
     2281 ggcgtgaacg tcacgctgga tgacggacgg atcatcaagg tgctcggcga catcgagaac
     2341 cccatgtatc acggctactc ctgcatcaag gggcgccagc tcgcggagca gcactaccat
     2401 ctcgaacggt tgctggcgcc tcacaagcgc agcaacggat cccacggaaa ggtcccgtcg
     2461 atgcaagcgc tggacgagat cgccgagcgg ctcggcgagc tcatcgatgc ccatgggccc
     2521 agaagcgtcg ccacgtacac gggcacctac tcgtttccct accccgccgg cacggccatg
     2581 gccaccgcgt tcatggatgc catcggctcg cccatgcgct tcacctccgg ctccatcgac
     2641 cagcccggca agcccatggc catcgccttt cacggccgct ggagcgccgg ccccccggcg
     2701 ttcgcggact ccgacgtctg gatgctggtg ggcgcgaacc ccgtggtgtc caagtggggc
     2761 ggcatcccac agtacaaccc gggcaaacgc ctgcacgatg cgttgaaacg cggcatgaag
     2821 ctcatcgtca tcgacccgcg agagacggaa tgcgcgaagc gagcccacct ccatctgcag
     2881 tgcaagcccg gcgaggaccc cacgattctc gccggcatca tgcacgtgat catcaacgaa
     2941 ggactgcacg acgccgagtt catcgactcg gacgtgaccg gcttcgacgc gctcaaggat
     3001 gccgtggccg acttcacgcc cgactacgtg gcccagcggg ccgagatacc caaggcgcag
     3061 ctgatcgagg cggcccgaac gttcgcagcg gcgagccgcg ggatgaccac ggccggcacc
     3121 gggcccaaca tggcgccccg agggacgctg accgagtatc tggtgctctc cctcaacacg
     3181 ctgtgcgggc gctggcttca ggccggcgag gtcatgccca accccttcgc gctcttgccc
     3241 gagcgccgtg gccgcgccca gcctgagcca aaacccgaac cgttcggtta cggcgagcgg
     3301 ctgagagttc gggggctgac cgagaacgtc agcggcctgt cggcagcggc cttagcggac
     3361 gagatactgc tggaaggcga aggccaggtg aaagcgctct tctgcgtcgg cggcaacccc
     3421 atggcggcct ggcccgacca gctgaagacc taccaggcca tgaaggcact cgagctcaac
     3481 gtctgcctgg acatcaagat gtcggccacc gccaagctcg cccacttcgt gctgccgccc
     3541 cggctcggcc tggaatcgcc cgccatctcg ttgccgaatg agaacatctg gttctacggc
     3601 ttctccaccg ggttccccga accttacgcc atgtaccagc cccagctcgt cgagccgcca
     3661 gccggctccg acctgctgga agagtgggag gtcttctacg gcgtcgccca gcgcatgggg
     3721 cttgggctca ccttcgccgg ggaggggctg gacatgaacc gcaa
//
LOCUS       DBNHQR010000547         5560 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_814837, whole genome shotgun sequence.
ACCESSION   DBNHQR010000547 DBNHQR010000000
VERSION     DBNHQR010000547.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5560)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5560
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_814837"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="AC16VR_12495"
     CDS             complement(<1..763)
                     /locus_tag="AC16VR_12495"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF018305.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6012021.1"
                     /translation="MADYSNHRERFVVPELCNAAAISLLVLFGELLVLVLLFAGGPIT
                     WLRLALMSLFVQWVVIMSAGALCWTRAWLSRFAVVPGAVLAFSMVMAITLFVGISADR
                     IMAPPGVEAINWMGILGQLLISGIITSLALRFLYVQQQLRAQEQSELQSRIQALQSRI
                     RPHFLFNSMNIIASLIATDPETAESVVEDLSELFRASLNDAGNQVPLQDELELCERYV
                     RIESLRLGDRLRVEWNVDDSGQNLKIPLLTLQPLLE"
     gene            789..2168
                     /gene="argH"
                     /locus_tag="AC16VR_12500"
     CDS             789..2168
                     /gene="argH"
                     /locus_tag="AC16VR_12500"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HIR6012022.1"
                     /translation="MSRDSKPWGGRFSEATDAFVESFTASVAFDKRLYHHDIRGSIAH
                     ANMLSRIGVLTEGERNEIVSGLQAIHKEIDEGELAWRVELEDVHMNVESRLIELIGET
                     GKKLHTGRSRNDQVATDIRLYLKDAIDELVALETQLLGALVDLAEAHADTVMPGFTHL
                     QPAQPVTFGHHLLAWFEMLHRDRDRLNDCRRRVDVMPLGSAALAGTTYPIDRAGVAAE
                     LGFGAVAENSLDAVSDRDFAIEFTSVAALTMNHFSRWSEELVLWTSQQFGFVELPDRF
                     CTGSSIMPQKKNPDVPELIRGKSGRVAGNLVALLTLMKGQPLAYNKDNQEDKEPLFDT
                     VDTLRDCLTALNGLVPAITPNRERMRSAAAAGFTTATDLADYLVRRDVPFRDAHEVVG
                     RAVAHAIGQGKGLDELTIEELRAFSDSIGEDVFSVLTLEGSVSARNHPGGTAPDSVRE
                     AAARARDRL"
     gene            2352..3626
                     /gene="lysA"
                     /locus_tag="AC16VR_12505"
     CDS             2352..3626
                     /gene="lysA"
                     /locus_tag="AC16VR_12505"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HIR6012023.1"
                     /translation="MDAFSITDGVLHAENVPLPRIAEAVGTPTYVYSTAHLKAQYQRL
                     RRALGELDADVCYAVKANANLAILRLFAEQGAGFDIVSGGELQRVISAGGDPARVIFS
                     GVGKSVAEIDFALKLGIRCFNVESASELDCLSERARVLKRQAPMSIRVNPDVDAGTHP
                     YISTGLRSNKFGIPPGQAVELYRRAQADEYLSVHGIDCHIGSQIGTIDPLIDALRNLL
                     ELTDRLSAEGIRLEHVDVGGGLGIRYGDEEELDVEAYGAALCELLRGRELKLLLEPGR
                     YLVGNGGVLLTRTRFLKPTTAETEKNFAVVDAAMNDLIRPALYQAWHEVRPVTPRAGA
                     AELCWDVVGPVCETGDFLAERRELALQEGDLLAVMSAGAYGMVQSSNYNTRPRPAEVL
                     VAGDRFRVVRRRETTRDQLALELEAEHAETWR"
     gene            3623..4474
                     /gene="dapF"
                     /locus_tag="AC16VR_12510"
     CDS             3623..4474
                     /gene="dapF"
                     /locus_tag="AC16VR_12510"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016703183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HIR6012024.1"
                     /translation="MKLEFAKMHGLGNDFMVLDLISQDFEPNREQIHAWADRRTGIGF
                     DQMLTISAPTDPDADFRYRIYNADGTEVEQCGNGARCAARFIAERKLSPKDTLVLETL
                     AGPISTALLDAETVEVDLGVPSTRAEDVPFSPEAACGAQGDAVGTFTLRAGEGEHEVT
                     PVSVGNPHGVLFVTQITDAPVDAVGASLTRHPFFPEGANIGFCQVVDPHFIRLRVYER
                     GVGETRACGTGACAAVVAGRLLGHLEDRVKVSLPGGKVRIAWQGPETTVRMAGPATLV
                     YEGHMSL"
     gene            4471..5145
                     /locus_tag="AC16VR_12515"
     CDS             4471..5145
                     /locus_tag="AC16VR_12515"
                     /inference="COORDINATES: protein motif:HMM:NF016243.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HIR6012025.1"
                     /translation="MTAAQDEPTRLTDKDVISYLRLDPDFFQRHSKLLSELNLPHDTG
                     GAVSLIERQVAILRERNMAMRRRMNELVQAAKANETLFEKTRSLTLELLHVDTWHALN
                     EVLATYVLVDFHADFVCCHLRNSEIRMDHVRGHGEELPNERYSRSIDPACVTLRADEL
                     EVLFPAENHDEGGSAVLAPLVLNEAIGCLAIGSRDPAHFESDMDTLFVAYIAEVVARV
                     VQRLSP"
     gene            5142..>5560
                     /locus_tag="AC16VR_12520"
     CDS             5142..>5560
                     /locus_tag="AC16VR_12520"
                     /inference="COORDINATES: protein motif:HMM:NF024886.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HIR6012026.1"
                     /translation="MTDPAVKRFQDYLRVEKRYSPNTLAAYRRDLERLGAVARVPWTE
                     IRGHHVSTFVAKLHAEGLAPRSIQRALSSIRSFYSYLEKIKLVASNPAAGIKAPKTRM
                     KLPKTLDTDQASQLFAFEAKTPIQKRDRAMAELLYGSG"
BASE COUNT         1020 a         1814 c         1849 g          877 t
ORIGIN      
        1 tttccagaag tggctgcaga gtcagcagag gaatcttgag attctggccc gagtcgtcca
       61 cgttccattc gacgcgcagc cgatcaccga gccgcaggga ctcgatgcgc acgtaacgct
      121 cgcaaagctc cagctcgtcc tgcagcggca cctgatttcc cgcgtcgttg aggctggcac
      181 gaaagagctc ggagaggtcc tccacgacag actccgcggt ctcggggtcg gtggcgatga
      241 ggcttgcgat gatgttcatg ctgttgaaca ggaagtgcgg gcgaatgcgc gactgcaacg
      301 cctggatgcg cgactggagc tccgactgct cctgagcgcg cagctgctgc tggacgtaca
      361 ggaagcgcag cgccagggaa gtgatgatcc cggaaatgag gagctggccg agaatgccca
      421 tccagttgat cgcttccacg ccgggcggag ccatgatccg atcggcgctg atcccgacga
      481 acagggtgat cgccatgacc atcgagaaag cgagcacggc gccaggcacc accgcgaacc
      541 gggagagcca ggctcgcgtc cagcacaggg ctcccgcgct catgatgaca acccactgca
      601 cgaacaggga catgagcgcg agccggagcc acgtgatggg gccgccggcg aacagcagca
      661 ccagaaccag aagctcaccg aagagcacga gcagggaaat ggccgccgcg ttgcaaagct
      721 cggggaccac gaaccgctcg cgatggttgc tataatcggc catccgctaa aggaaccctc
      781 ttgtcaccat gagccgagat tcaaaaccct ggggcggccg cttttcggaa gccaccgatg
      841 cgttcgtcga atcgttcacg gcctcggtgg cattcgataa gcgtctttac catcacgata
      901 tcaggggctc catcgcccac gccaacatgc tgagccggat cggcgtgctc accgaaggtg
      961 agcgcaacga gatcgtatcg ggattgcagg cgattcacaa agagatcgac gaaggcgagc
     1021 tcgcctggcg ggttgagctc gaggacgtcc acatgaacgt ggagtcgcgc cttatcgagc
     1081 tcattggaga aaccgggaaa aagctgcaca cgggccggtc ccgcaacgac caggtcgcca
     1141 ccgatatccg gctctacctg aaggacgcca tcgacgagct ggtcgctttg gaaacccagc
     1201 tgctcggcgc gctggtggac ctggccgaag cccacgcgga caccgtcatg cccggtttca
     1261 cgcatctgca gccggcgcag cccgtaacgt tcggccacca tctgctggcg tggttcgaga
     1321 tgcttcaccg cgaccgcgac cgcctcaatg actgccgccg gcgcgtggac gtcatgcccc
     1381 tgggcagcgc ggcgcttgcc ggcaccacct atcccatcga tcgggccggt gtcgctgccg
     1441 agctcggatt cggcgccgtg gccgagaact cgctggacgc ggtgagcgac cgggacttcg
     1501 ccatcgagtt cacgtcggtt gccgcgttga cgatgaacca cttttctcgc tggagcgagg
     1561 agctggtgct gtggacgtcc cagcagttcg gattcgtcga gctgcccgat cgtttctgca
     1621 cgggctcgag catcatgccg cagaagaaga accccgacgt ccccgagctg atccggggca
     1681 agtcgggcag ggtggccggc aacctggtgg ctctgctgac gctgatgaaa ggccagccgc
     1741 tggcctacaa caaggacaac caggaggaca aggagccgct gttcgacacg gtggacaccc
     1801 tgcgcgattg cctgacggct ctgaacggcc tcgtcccggc catcaccccc aatcgggagc
     1861 ggatgcgttc ggccgccgcc gcgggcttta ccaccgcgac ggacctggcc gattacctgg
     1921 tgcggcgcga tgtgcccttc cgggacgccc acgaggtggt gggccgcgcc gtggcgcacg
     1981 cgatcgggca gggcaagggt ctcgacgagc tgacgatcga ggagctgcgc gcgttcagcg
     2041 acagcatagg cgaggacgtg ttttccgtgc tcacgctcga ggggtccgtc agtgcgcgca
     2101 atcacccggg cggcaccgcc cccgacagcg tgcgcgaggc ggcagcccgc gcccgcgacc
     2161 ggctttgacc gcggccgctg cgccgccatg aggcggtgta tactgactcg ttcggcctag
     2221 ctgcgagcca ccggtgcgct ggatcctgtt aatcattctt gcctctcagg cggtcatgtg
     2281 cggtcagaag ggtcccctgg agctgcccga cgctcagtca gaagcgcccg ccgccagcca
     2341 ccggccctga cgtggacgcc ttctccatca cagacggggt gctccacgcg gagaacgtgc
     2401 cgctgccgcg gattgccgag gcggtcggca cgccgacgta cgtctactcg accgcccacc
     2461 tgaaggcgca gtaccagcgg ctgcgccgcg cgctcggcga gctcgacgcg gacgtctgct
     2521 acgcggtcaa ggccaacgcc aatctggcca ttctgaggct cttcgctgag cagggcgccg
     2581 gtttcgacat cgtctccggc ggcgagctgc agcgggtcat cagcgcagga ggcgaccctg
     2641 ccagggtgat tttctccggg gtaggcaagt cggtggccga gatcgacttc gcgctcaagc
     2701 tcggcatccg ctgcttcaac gtggagagcg ccagcgagct cgactgcctg agcgagcggg
     2761 cacgggtgct caagcgccag gcgcccatgt ccatccgggt caacccggac gtggatgccg
     2821 ggacgcatcc ctacatctcc acggggttac gcagcaacaa gttcggtata ccgccgggcc
     2881 aggcggtcga gctgtacagg cgggcgcagg cagacgagta cctatccgtg cacgggatcg
     2941 actgccacat cggctcccag atcggcacca tcgacccgct gatcgacgcc cttcgcaacc
     3001 tgctggaact caccgaccgg ctgtccgcgg agggaatccg cctggaacac gtggacgtgg
     3061 gcggcgggct gggcatccgc tacggggacg aggaggagct cgacgtcgag gcatacggtg
     3121 cggccctttg cgagctgctg cgcggccggg aactcaagct cctgctggaa cccggcaggt
     3181 atctggtggg gaacggcggc gtgctgctga cccgaacgcg attcctgaaa ccaacgacgg
     3241 ccgagaccga gaagaacttc gcggtggtcg acgctgccat gaacgatctg atccgcccgg
     3301 cgctgtacca ggcgtggcac gaggtccgtc ccgtcacgcc gcgagcgggg gcggccgagc
     3361 tgtgctggga cgtggtgggc cccgtctgcg agaccggcga cttcctggcc gaacgcagag
     3421 aactggccct tcaggaaggc gaccttctgg ccgtcatgtc cgccggggcc tacgggatgg
     3481 tgcagagctc caactacaac acgcgtccca gacccgcgga ggttctggtc gccggagacc
     3541 gcttccgggt ggtgcggcgc cgcgaaacca ctcgcgatca gctcgccctg gagctcgagg
     3601 cggagcacgc ggagacgtgg cgatgaagct cgagttcgcg aagatgcacg ggctcggtaa
     3661 cgacttcatg gtgctggacc tgatcagcca ggatttcgag ccgaaccggg aacagattca
     3721 cgcctgggcc gatcgccgca ccgggatcgg cttcgatcag atgctcacca tctcggcgcc
     3781 caccgacccg gatgcggact tccgctaccg catctacaac gcggacggca ccgaggtgga
     3841 gcagtgcggc aacggcgccc gatgcgcggc cagattcatc gccgagcgaa agctgagccc
     3901 gaaagacacc ctggtgctcg agacgttggc ggggcccata tccacggccc tgctggacgc
     3961 cgagaccgtg gaggtcgatc tgggggttcc aagcacccgg gccgaagacg tgccgttcag
     4021 cccggaggcg gcgtgcggcg cgcagggcga tgcggtcggc accttcacgt tgcgcgcggg
     4081 ggagggcgag catgaggtga ccccggtctc cgtcgggaat cctcacgggg tgctcttcgt
     4141 aacgcagatc accgatgcgc ccgtcgacgc ggtgggggcc agcctgaccc gccacccgtt
     4201 ctttcccgaa ggcgcgaaca tcgggttctg ccaggtggtg gatccgcatt tcatccggct
     4261 gcgcgtctac gagcgagggg tgggcgaaac gcgggcttgc ggaacgggcg catgcgccgc
     4321 cgtcgtcgcc ggccggctgc tgggccacct ggaagaccgg gtgaaggtat cgttgccggg
     4381 aggcaaggtc cgcatcgcct ggcagggccc ggagaccacg gttagaatgg ccggacccgc
     4441 cacactggtt tacgaaggcc acatgagtct atgaccgcgg cccaggacga gccgacgagg
     4501 cttaccgaca aagacgtcat ttcttacctg cgactcgacc ccgacttctt ccagcggcac
     4561 tcgaagctgc tttccgagct caacctgccc cacgacacgg gcggcgcggt ctcgctgatc
     4621 gagcgtcagg tagccatcct tcgcgaacgc aacatggcga tgcggcgccg gatgaacgag
     4681 ctcgttcagg cggccaaggc caacgagacg ttgttcgaga agacgcgttc cctgacgctg
     4741 gagctgctcc acgtggacac gtggcacgct ctgaacgagg tgctcgcgac ctacgtgctg
     4801 gtggatttcc atgcggactt cgtatgctgc catctgagga attcggagat ccgcatggac
     4861 cacgtgcggg gccacggcga agaacttccc aacgagcgct actccagaag catcgatccc
     4921 gcgtgcgtca ccctgcgcgc cgacgagctc gaggtgcttt tccccgcgga gaatcacgat
     4981 gagggcggca gcgcggtgct ggcaccgctg gtgctcaacg aggcgatcgg ctgtctcgcc
     5041 atcggcagcc gcgatcctgc tcacttcgaa tcagacatgg atacgctgtt cgtcgcctac
     5101 atcgcggagg tggtcgcgcg ggtggtccag cgcctgtcgc cgtgaccgat cccgccgtca
     5161 agcggttcca ggactacctg cgggtcgaga agcgctactc acccaacacg ctggccgcct
     5221 accggcggga tctggaacgg ctgggcgcgg tggccagggt gccctggacc gagatccgcg
     5281 gacaccacgt gagcacgttc gttgccaagc tgcacgccga agggctggct ccgcgaagca
     5341 tccagcgcgc cctgtcgagc atccgttcct tctacagcta tctggagaag atcaagctgg
     5401 tcgccagcaa ccccgcagcc ggcatcaaag cgccaaagac ccggatgaag ctgcccaaga
     5461 cgctcgatac ggatcaggcc tcacagctgt tcgccttcga ggccaaaacg ccgattcaga
     5521 aacgcgaccg cgcgatggcc gagctgctct acggcagcgg
//
LOCUS       DBNHQR010000548         2665 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1067336, whole genome shotgun sequence.
ACCESSION   DBNHQR010000548 DBNHQR010000000
VERSION     DBNHQR010000548.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1067336"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="AC16VR_12525"
     CDS             complement(<1..329)
                     /locus_tag="AC16VR_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012027.1"
                     /translation="MWSTLLLASVFATSVLSGVLGMAGGMILMAILVSLMSVPAAMII
                     HGVVQATSNGSRAWFLRAHIRWRVLPPYLLGAAVTLAAFAALTLVPEAGLVLVLVGIF
                     PWLARVVP"
     gene            435..1289
                     /locus_tag="AC16VR_12530"
     CDS             435..1289
                     /locus_tag="AC16VR_12530"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR6012028.1"
                     /translation="MIENLETLVTLARTGTMMETSTVLKISQSAVSKRIAALERYYDR
                     DLIERHGRRVVLTHHGTRLVEKVTPVISELRSVFLEDNALRKGKIIIGVSEAILSSWG
                     PRLFARVRSELPEVELEFHAQRSPVVLDRIRSGEYMVGICTGSPDADTDLQSEVIRLE
                     PMVIIPSALAPLDYEVGDELDVITIESRSGAWRSIEDDMRRQNLRRVVPLESFFGVAQ
                     MALAGFGHGLVPEGVAQTLGVTAGERIPLDSAGLNRPVRFVARKSMFSQQLVQSFYQA
                     VSRFAAEV"
     gene            complement(1343..2353)
                     /gene="hemH"
                     /locus_tag="AC16VR_12535"
     CDS             complement(1343..2353)
                     /gene="hemH"
                     /locus_tag="AC16VR_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019406282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - protoporphyrin ferrochelatase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HIR6012029.1"
                     /translation="MTRGLLLVNLGTPLSSDVRDVRAFLGEFLMDRYVLDVPWLLRRL
                     IVTGFILPFRPNQTARAYAKIWNAAGPETGSPLLHYSELCRHGVEERTGMPTALAMRY
                     GTPSVRDAIAALLGHGVRQITMLPLYPQHADSTITTSIEAVRRSLPTNVSLTVVPPFY
                     SQPDYLDAQAAAIRENLPASWDHLLFSYHGLPERHITKADPTGNHCLALENCCQIPSE
                     AHATCYRHQVYRTSELLAQRLGLSEDQYSVSFQSRLGRLPWLTPYTDQVLEALPGKGV
                     RRLAVTCPAFVADNLETLEEIGIAGRESFLAAGGESFSLVPCLNDRADWIDAIVRIAG
                     AA"
     gene            2446..>2665
                     /gene="hisH"
                     /locus_tag="AC16VR_12540"
     CDS             2446..>2665
                     /gene="hisH"
                     /locus_tag="AC16VR_12540"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="with HisF IGPS catalyzes the conversion of
                     phosphoribulosyl-formimino-5-aminoimidazole-4-carboxamide
                     ribonucleotide phosphate and glutamine to
                     imidazole-glycerol phosphate,
                     5-aminoimidazol-4-carboxamide ribonucleotide, and
                     glutamate in histidine biosynthesis; the HisH subunit
                     provides the glutamine amidotransferase activity that
                     produces the ammonia necessary to HisF for the synthesis
                     of imidazole-glycerol phosphate and
                     5-aminoimidazol-4-carboxamide ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HIR6012030.1"
                     /translation="MSHAIIIDYDAGNLRSVQRACSEVGIDAEISADADRVAAADRLI
                     FPGVGAAGSAMRSVRRNGLDQALRSALAG"
BASE COUNT          486 a          802 c          928 g          449 t
ORIGIN      
        1 gggacgacgc gagccagcca gggaaaaatc ccgacgagaa cgagcaccag gcctgcttcg
       61 gggacaagcg tcagggctgc aaaagcggca agggtgacgg ccgcgcccag cagataaggg
      121 ggcagcaccc gccaccggat gtgtgcgcgc agaaaccagg ccctggaccc gttcgaggtg
      181 gcctgaacca cgccgtgaat gatcatggcg gcgggcacgg acatcaggga gaccaggatg
      241 gccatcagga tcatgccgcc cgccatcccc agcacgccgg agagcacgct ggttgcgaag
      301 acgctcgcga gcagaagggt cgaccacatg atcacgcccg gcaaaatgtg ctcagggtgc
      361 cccaaccata accatgaaaa aagagaatgg atggaatatg atcgtcgccg aaatcgaatc
      421 aattcaatcg ggccatgatc gaaaacctcg agactctggt caccctggcg cgcacgggca
      481 ccatgatgga gaccagcacg gtgctgaaga tttcccaatc cgccgtgagc aaacgcatcg
      541 ccgcgctcga gcgctactac gaccgcgatc tgatcgagcg ccatggccgg cgggtggtgc
      601 tcacccacca cggaacgcgg ctggtggaga aggtcacccc ggtgatctcc gagctgcgca
      661 gcgtgttcct ggaggacaac gcgctgcgca agggcaagat catcatcggc gtttccgagg
      721 ccatcctctc cagttggggc cctcgactgt ttgcccgggt gcgctccgag ctgccggagg
      781 tggagctcga gtttcacgcc caacgttcgc cggtggtgct cgaccggatc cgctccggtg
      841 agtacatggt cggcatctgc acgggctcgc cggacgcgga tacggacctg cagagcgagg
      901 tcatcaggct cgagcccatg gtcatcatcc cgtctgcgct ggcgcctctc gattacgagg
      961 tgggtgacga gctggacgtg atcaccatcg agtcgcgctc gggtgcctgg cgctccatcg
     1021 aggacgacat gcgccggcag aacctcaggc gcgtcgtgcc gctggagtca tttttcggcg
     1081 tggcgcagat ggccctggcc ggttttggtc acgggctggt gccggagggg gttgcccaga
     1141 cgctgggggt gacggccggc gaacggatac cgctggacag cgcagggctg aaccggcccg
     1201 ttcgtttcgt tgcccggaaa tccatgttct cgcagcagct ggtacagagt ttctaccagg
     1261 cggtttcccg tttcgccgcc gaggtgtgac cgcttcgaag acccgttttt ccggtgatga
     1321 ccgatgtggc cggaggcggg ctctaggccg ccccggcaat tcggacgatg gcgtcgatcc
     1381 agtccgcgcg gtcgttcagg cagggaacca gcgagaacga ctctccgccg gcagccagga
     1441 agctctcgcg cccggcgatg cctatttcct ccagagtttc cagattatcc gcgacgaagg
     1501 ccgggcaggt gacggcgaga cgcctgaccc cttttcctgg cagggcttcc agcacctggt
     1561 cggtataggg ggtgagccac ggcagccgtc ccagccgcga ctgaaagctc acgctgtact
     1621 gatcctcgga gaggcccagg cgttgggcaa ggagctcgga ggtgcggtag acctgatgcc
     1681 ggtagcaggt ggcgtgcgcc tcggagggga tctggcagca gttctccagg gcaaggcaat
     1741 ggttgccggt gggatctgcc ttggtgatgt gccgctcggg cagaccgtgg taactgaaga
     1801 gcaggtgatc ccaggacgcc ggcaggttct ctcggatcgc ggcggcctgg gcatccagat
     1861 agtccggctg cgagtagaaa ggcggcacca cggtcaggct aacgttggtt ggcagacttc
     1921 gtcgtacggc ctcgatgctg gtggtgatgg tggagtcggc gtgctgcggg tacaggggaa
     1981 gcatggtgat ctgccgcacg ccatgtccga gcagggcggc gatggcgtcc cgcacgctgg
     2041 gggtcccgta gcgcatggcg agagcagtag gcatccccgt gcgttcctcg accccgtgac
     2101 ggcatagctc cgagtaatgc agaaggggcg agccggtttc cggtcctgcc gcattccaga
     2161 tcttggcgta ggcccgggcg gtctggttgg gacggaacgg cagaatgaac ccggttacga
     2221 tcaggcgccg cagtagccag ggcacgtcga ggacgtatcg gtccatcaga aattcgccca
     2281 ggaacgcgcg cacgtcgcga acgtcactcg aaagcggggt gccgaggttg accagcagca
     2341 ggcctcgggt cacgggaggt ctccggtgaa tgtagtttgc ccaagcgcga tgggatagta
     2401 tccgcgctcg ttttggatgt cacttttcct accggatccc acgggatgtc ccacgccatc
     2461 atcatcgact atgacgccgg caacctgcgc agtgttcagc gggcctgctc ggaggtcggc
     2521 atcgatgcgg agatcagcgc cgatgccgac cgcgtggcgg ccgccgaccg gctgatcttc
     2581 ccgggcgtcg gcgcggcggg gagcgcgatg cgaagcgtgc gccgcaacgg gctggatcag
     2641 gcgttgcgct cggccctggc gggcg
//
LOCUS       DBNHQR010000549         4724 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1574434, whole genome shotgun sequence.
ACCESSION   DBNHQR010000549 DBNHQR010000000
VERSION     DBNHQR010000549.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4724)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4724)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4724
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1574434"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="AC16VR_12545"
     CDS             complement(<1..754)
                     /locus_tag="AC16VR_12545"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HIR6012031.1"
                     /translation="MTTHDESTGPEGYRVAEVEAWIAENVQALKPPFEWTRLQGGHSN
                     LTYLLVDGDGNRAVIRRPPQGELLPKAHDMSREWALISALGPTPVPVPAALGFCESPD
                     VTGAWFYVMGFVNGHPLYNSDDTRQWVPREHRRKMAFSFIDVLADLHAVDPDEVGLGE
                     LGKKENYVGRQLKTWYRSWTSSIEAADYDDPRAHELQRYLLENIPEQGPARVVHGDYG
                     LHKCLVGPDCTIAAVVDWEISTLGDPLADLAYA"
     gene            complement(802..1254)
                     /locus_tag="AC16VR_12550"
     CDS             complement(802..1254)
                     /locus_tag="AC16VR_12550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HIR6012032.1"
                     /translation="MSERQLPEGLPDWIVEHIRLYREDPDKGHMWDSTALGGPGVLPT
                     LLLTTRGRRSGEPRILPLIYAEVQGGYAIIASKGGFPTHPAWYLNLVDEPECGVQVGQ
                     DEFRARARTADGEEREAIWAQMEGIYPPYRDYQAATERRIPVVVLERI"
     gene            complement(1336..2553)
                     /gene="aroQ"
                     /locus_tag="AC16VR_12555"
     CDS             complement(1336..2553)
                     /gene="aroQ"
                     /locus_tag="AC16VR_12555"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma subclass chorismate mutase AroQ"
                     /protein_id="HIR6012033.1"
                     /translation="MTLPGLSRRWWCVALCLTVPVCAWAGDPGVAELAHQRLALMQDV
                     AAHKWVNGLPVEDGAREAKVLESAEAAALRFGVDVSATRALFEAQIEAAKEIQRYWFA
                     RWALGSPPRSAPDLVGDLRPRLLALGDLILAGLAAAPGRAGSVPPLPGLSESSRRRLS
                     DALSAVEPYPSRLEQVRRSGVLRIGTTGDYPPFSHRVDDDGPLGGADVDLGRLLAHSL
                     GAEARFVPTSWPTLMDDLAAGRYDVAMSGVSRTAARERVAAFTRAYHVGGKTPIARCS
                     ERERFSSLAQIDRRGVRVIVNPGGTNELFVDNNLTLADKVLHTDNRSIFQALIEGRAD
                     VMITDRIEVRLQVRLHEGVLCATTPRNFNHQEKAYLLPRDAAWHAYVDAWLVSMLAQG
                     VVERTIARHLGED"
     gene            complement(2550..4436)
                     /locus_tag="AC16VR_12560"
     CDS             complement(2550..4436)
                     /locus_tag="AC16VR_12560"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIR6012034.1"
                     /translation="MKKAALPENEGERLERLRDYDILDTLPEQDYDDITYLASQICDT
                     PIAVISLVDDDRQWFKSAQGLDVSETPRDVAFCAHTILEPDKLMVVEDASQDDRFADN
                     PLVAGDLGIRFYAGAPLVTASGHALGTLCVIDQKPRGLTDQQRKTLAVLARQVVALLE
                     LRRSVAELKRSSDALHESHQFLALRGQQLNRSRDELAEAVRVLEGQADVIEQDLHRAE
                     VIQRSLLPHEVPRLEDFSVRSLYRPGHTVGGDLYDVVSIADRYLAIVVADACGHGVSA
                     AMLSVLFKQHLRLQDPASGIPYPPAWALERINRSVVANRPAPGVFLTAVYALVDTQTR
                     KLKLGSAGHPPVICLRASGEVESFAHTGPALGLDESSTYEEAEIELGVGDKLLFYTDG
                     LLDICESSPTVEALGQAMVSVADKPNLLEQVLMEVTHGRARPDCDDVTMVLLDAAPGE
                     SVFNESVEGLELQEMPTTASPTISWAASEDGVFLILEGWVTWLCGETLFNAAMPLIKA
                     HRRLVIDLELCEHMDSTMFGTLHELIQEADRLGSAVVLQHVGPRQMEDFEELSMAAAL
                     AHVVTDPVPVPSQRTPLELRSTSIEGQQRRLLQAHEALLKLSDANRDQFGNLVDNLRE
                     ELEG"
BASE COUNT          828 a         1593 c         1547 g          756 t
ORIGIN      
        1 gcgcgtaggc cagatccgcc agcggatcgc ccagggtaga tatctcccaa tccaccaccg
       61 cggcgatggt gcaatccgga ccaaccaggc atttgtgcag gccgtaatcg ccgtgaacca
      121 cgcgggccgg cccctgctcg ggaatgttct ccaggaggta gcgctgcagc tcgtgggcgc
      181 gcgggtcgtc gtagtcggcc gcctcgatgg acgaggtcca ggagcgatac caggtcttga
      241 gctgacggcc cacgtagttc tccttcttcc ccagttctcc caggcccacc tcgtcggggt
      301 ccacggcgtg cagatcggcg agcacgtcga tgaacgagaa cgccatcttc cgacggtgct
      361 cccggggtac ccattgccgg gtgtcatcgg agttgtacag cgggtgcccg ttcacgaagc
      421 ccatgacgta gaaccaggcg ccggtaacgt ccgggctctc gcagaatccc agcgcggcgg
      481 gcacgggcac cggtgttggc ccaagggccg agatcagggc ccactcgcgg ctcatgtcat
      541 gggctttcgg cagcagctcg ccctggggcg gcctgcggat gacggcccgg ttgccgtcac
      601 cgtccacgag caggtaggtg aggttggagt ggccgccctg aaggcgcgtc cactcgaaag
      661 gcggcttcag ggcctgcacg ttctcggcga tccacgcctc cacctcggca acccggtagc
      721 cctccggacc cgtgctctca tcatgcgtcg tcatcgtatc tccccccgcg cgacggcggc
      781 gcccccgcgc cttccggccc ctcagatcct ttccagcacc accaccggta tccgccgctc
      841 ggtggcggcc tggtaatccc ggtagggcgg atagatccct tccatctgcg cccagatggc
      901 ttcccgttcc tcgccatcgg cggtgcgggc ccgggcgcgg aactcgtctt ggcctacctg
      961 aacgccgcat tccggttcgt ccacgagatt cagataccac gccgggtgcg tcggaaaccc
     1021 gcctttggag gcgatgatag cgtagccgcc ctgcacctct gcgtagatga gggggaggat
     1081 gcgcggctcc cccgacctgc gcccacgggt agtcagcagc agcgtgggca gcacgcccgg
     1141 accgcccagc gcggtggaat cccacatgtg gcccttgtcc ggatcttccc ggtacagcct
     1201 gatgtgctcg acgatccaat cgggcaagcc ttccggcagc tgccgttcac tcatgtccgc
     1261 ttcctcccct ttcagtcggc tcgatgctct catggtgcac cagagaagcc gtccccgcca
     1321 gccgtcgccc tcgcgtcaat cctcgcccag atgccgggcg atggtgcgct ccaccacgcc
     1381 ctgagcgagc atgctgacga gccaggcgtc aacgtaggcg tgccatgccg catcccgggg
     1441 caggaggtag gctttctcct ggtggttgaa gttacggggc gtggtggcgc aaagaacccc
     1501 ttcgtgcagg cggacctgca ggcgcacctc gatacggtcg gtgatcatga catccgcacg
     1561 gccttctatc agagcctgaa agatagagcg gttgtctgtg tgcagaacct tgtcagccag
     1621 cgtcaggttg ttgtccacaa agagctcgtt ggtgcccccg gggttgacga tgacccgaac
     1681 cccgcggcgg tcgatctggg cgaggctcga gaaccgttcc cgctccgagc acctggcaat
     1741 aggcgtcttc ccgccgacgt ggtacgcgcg ggtaaacgcg gcaacccgct cgcgcgccgc
     1801 cgtccgagac accccgctca tggcgacgtc gtagcgtcca gcagccagat cgtccatgag
     1861 ggtaggccag gaggtaggga cgaatctggc ttccgctccg agcgaatggg ccagcaagcg
     1921 accgagatcg acgtccgccc cgcctaaggg cccgtcatcg tcaacacggt gcgaaaacgg
     1981 ggggtagtct ccagtggtgc cgatgcgcag aacgcccgag cggcgaacct gctccagacg
     2041 gctcggatag ggctccacgg ccgacagcgc atcgctcaga cgccgacgcg acgattcgct
     2101 gagccccggg agcggcggaa ccgaccctgc gcgacccggc gccgcggcga gcccggccag
     2161 gatgaggtcc ccgagggcaa gcagccgggg ccggagatcc ccgacgaggt cgggggccga
     2221 gcgcggcggt gagccaaggg cccagcgagc aaaccagtag cgttggatct ccttggccgc
     2281 ttcgatctga gcttcgaaga gcgcccgggt ggcagacacg tcgacgccga atcgaagcgc
     2341 agcagcttcc gcgctttcga gcaccttcgc ctcgcgcgcg ccatcctcga ccgggaggcc
     2401 gttaacccac ttgtgagccg ccacgtcctg catgagcgca aggcgctgat gggcaagctc
     2461 ggcgaccccg gggtcgccag cccacgcaca tacaggaaca gtcagacaca gggcgacgca
     2521 ccaccatcgc cgacttaacc cgggaagcgt cagccttcga gctcctcgcg caggttgtcc
     2581 accaggttgc cgaactggtc gcggttcgca tcgctgagct tgagcagcgc ctcgtgagcc
     2641 tggagcagac gtcgctgctg accctcgatg ctggtgctgc gcagctccag cggcgtgcgc
     2701 tgggatggca ccgggaccgg gtcggtgacc acgtgggcaa gcgcagcggc catgctgagc
     2761 tcctcgaaat cttccatctg ccgcgggccc acatgctgca ggaccaccgc ggatccgagc
     2821 cggtcggctt cctggatcag ctcgtgcagg gtgccgaaca tggtgctgtc catgtgctcg
     2881 caaagctcga ggtcgatgac aaggcgccgg tgcgctttga tcaacggcat ggccgcattg
     2941 aacaacgtct ccccgcacag ccaggtcacc cagccctcca ggatgagaaa gaccccgtcc
     3001 tccgaggctg cccagctgat cgtcggcgag gccgtggtcg gcatctcctg cagctcaagt
     3061 ccctccacag actcgttgaa cacgctttcg ccgggagcgg catccagcag caccatggtc
     3121 acgtcgtcgc aatccgggcg ggcgcggccg tgagtcacct ccatgagcac ctgctcgagg
     3181 aggttcggct tgtcggctac cgagaccatg gcctggccga gcgcttccac cgtgggggaa
     3241 gattcgcaga tgtccagcag cccgtcggtg tagaacagaa gcttgtcgcc cacgcccagc
     3301 tcgatttcgg cctcctcgta agtcgaactc tcgtcgagcc ccaacgccgg gcccgtgtgg
     3361 gcaaacgact cgacctcgcc gctcgcgcgc agacaaatta ccggcggatg accggcggag
     3421 cccagcttga gctttcgggt ctgagtatcc acaagcgcgt agacggcggt cagaaacacg
     3481 ccgggcgcag gccggttggc gaccacggaa cggttgatgc gctccagcgc ccacgccggc
     3541 gggtaaggaa tgcccgacgc cgggtcctgc agccgcaggt gctgcttgaa gaggaccgag
     3601 agcatcgcgg cgctgacacc gtgaccgcag gcgtcggcga ccacgatggc gagataacgg
     3661 tccgcgattg aaaccacgtc gtacagatcc ccgcccaccg tgtgccccgg gcgataaagc
     3721 gatcgcacgc tgaagtcctc gagccggggg acctcgtggg ggagcagcga acgctggatc
     3781 acttctgccc gatgcagatc ctgctcgatc acgtccgcct gaccctccag cacccgcacg
     3841 gcttcagcaa gctcgtcccg cgacctgttg agctgctgcc cccgaagggc gagaaactgg
     3901 tgagactcgt gaagggcatc cgagctacgc ttaagctcgg cgacggaacg ccgcaattcg
     3961 aggagcgcga ccacctggcg agcgagcacc gcgagcgttt tgcgctgctg atcggtcagt
     4021 ccccggggtt tctggtcgat gacgcacaac gtgcccaggg cgtgacccga cgcggtgacg
     4081 aggggggcgc cggcatagaa gcgtatgcca agatcgccgg cgaccagagg gttgtccgca
     4141 aagcggtcat cctggctggc gtcctccacc accatgagtt tgtcgggctc gagaatcgtg
     4201 tgtgcgcaga acgcaacgtc tctcggtgtc tcggagacat ccaagccctg ggccgacttg
     4261 aaccactgac gatcgtcatc caccaggctg atgacggcta tgggggtatc gcagatctgc
     4321 gaagcgagat aggtgatgtc gtcgtagtcc tgctcgggaa gcgtgtcgag aatgtcgtag
     4381 tcacgcagcc gctcaagacg ttcgccctcg ttctcaggaa gcgcagcctt cttcatgacg
     4441 tacagattac gcctttaagt ctgccaggcg aagccgcaaa cgctatctgg cgcgctcagt
     4501 cgagccgcat gggcgtcggc ctgccggcgc gaaccccgcc gccccgcctg gaaatggtcc
     4561 cgggatcgaa ggccggccgg gacgtgcggg ccgacggagc gggctgagaa ggccgggagg
     4621 tgcgcgccga cggggcaggc tgcaagcgcc gggacgtggg aaccgccggc cgcggcggcg
     4681 aagatcgagg catgaacgcc atcggagccg gccgcgaggg caca
//
LOCUS       DBNHQR010000550         3471 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_181791, whole genome shotgun sequence.
ACCESSION   DBNHQR010000550 DBNHQR010000000
VERSION     DBNHQR010000550.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_181791"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1501
                     /locus_tag="AC16VR_12565"
     CDS             <1..1501
                     /locus_tag="AC16VR_12565"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6012035.1"
                     /translation="IQQWIIDGLVTRDPVTLEWLPHVAESWNISEDGLTISFVVRDGV
                     VFSDGEPLTAEDVAFSWRFVMDEKIAAPRARAYLSRIDDVTVDGRTVTFRFTEPYFGS
                     FSLAGNLGILPRHFYSKYLESVAAAEEYNTSTGLLLGSGPYRLPSPTAWKPGDLIELV
                     RNERYWGWVPAPFDRRIWKTINSDAAQLTEFKNGGIDVYAALPLEYRDLTQNEEIMKR
                     TRSYEYYNPRGGYSYIAWNNLRDGKPTAFADRRVREAMTYLTDRERVIDEVLLGYAQP
                     ANGPFNPLGKQADPSLSVRGYDLDKAKALLAEAGYLDRDGDGVLESEAGEPLAFKLTY
                     PSGSDNYKRIMLLLKDLYVQAGVLMEPDPVDWPVLINTLNTKNFDAVSLAWTSGFEID
                     IYQMLHSSQTEPGGDNFISYINPGLDELIETARQEMDEEARMAIWHQVHREIWAEQPY
                     TYLFRTKSLIFADRRIRNVQVVRAGLNEGGLWNMPMEWYVPTDAQKYSD"
     gene            1535..2608
                     /locus_tag="AC16VR_12570"
     CDS             1535..2608
                     /locus_tag="AC16VR_12570"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6012036.1"
                     /translation="MVNYVIRRLLLIFPTLLGITILVFAVMAAAPGGVGASLLSRSGD
                     LRPEERAAMERYINERYGLDKPMPMQYLRWLNNVSPIGFRKDENNEDAGFGFKVPDLG
                     MSFAKNRPVTQLVAQALPITLLLNALSVPLVFILSIVMGVYTARFRGQALDVGIGTAN
                     LALWSIPTIWMGVLLIGLLASEEYARIFPTSGLESLRADRWTFLPVVTEQGWQRGYLL
                     DVVWHLVLPIVCLTYTGFAVLTKLTRSAVLENLQADYARTARAKGASERTVLWYHVFR
                     NSLLPLLTVAASILPGMLVGSVIVESIFSIDGMGRLVVEAVQLRDREVVLSITLITSL
                     LTLLSYLIVDICYAAADPRVSFE"
     gene            2605..>3471
                     /locus_tag="AC16VR_12575"
     CDS             2605..>3471
                     /locus_tag="AC16VR_12575"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6012037.1"
                     /translation="MTETAITRAVEKVGEESFSRRVTRDTLRNTGAKIGIAWVATVAV
                     LAVFAPFIANSHPYLMRVEGMWSSPLLAHLSWADVTMLVTFLAVVAAWRLRRRFGPGQ
                     LLVGVGVISLVTLLAAQQLVQVPRAVIYDQYRVGLAEGGIETAYFAPVPFSPTDRSRD
                     NPAPLHEPDSVHWFGTDEDGADVLSRMIHACRIALGIGLVATGIALALGIVYGGIMGF
                     FSGWLDIAMMRFLEIFESIPSLFLLLTIVAFFGRNIYLIMVVIGLTSWPGYARFLRAE
                     FLKLRNQDYVQAA"
BASE COUNT          595 a         1203 c         1061 g          612 t
ORIGIN      
        1 cattcagcag tggataatcg acggcctggt gacccgggac ccggtcaccc tggagtggct
       61 gccccacgtg gccgaatcgt ggaacatcag cgaggacggg ctgacgatca gcttcgtcgt
      121 ccgcgacggc gtggtcttct ccgacggcga gcccctcacc gcggaggacg tggctttcag
      181 ctggcgcttc gtcatggacg agaagatcgc cgcgccgcgg gctagggcct acttatcccg
      241 catagacgac gtcaccgtcg acggccgcac ggtgaccttc cgcttcacgg agccctactt
      301 cggttccttc tctctggccg ggaacctggg cattctgccc aggcacttct actcaaagta
      361 tctggagagc gtggccgcag ccgaggagta caacacctcc accgggctgc tgctcggctc
      421 ggggccgtac cggctgccct cgccaaccgc ctggaagccg ggcgatctca tcgagctggt
      481 gcgcaacgag cgctactggg gctgggtacc cgcgcccttc gaccggcgta tctggaagac
      541 catcaactcc gacgcggccc agctcaccga gttcaagaac ggcggcatcg acgtttacgc
      601 cgcgctgccc ctggaatatc gagatctcac acagaacgaa gagatcatga agcgaaccag
      661 gtcgtacgag tactacaacc cgcgcggcgg ttactcctac atcgcctgga acaacctgcg
      721 cgacggcaaa cccaccgcgt tcgccgatcg cagggtccga gaagccatga cctacctgac
      781 ggaccgggag cgcgtcatcg acgaggtcct gctcggctac gcccagcccg ccaacggccc
      841 gttcaacccc ctgggcaagc aggccgaccc gagcctgtcc gttcgcggct acgatctgga
      901 caaggcgaag gccctgctcg ccgaagccgg ctacctggac cgcgacggcg atggggtcct
      961 cgagtccgag gccggcgagc cgctggcctt caagctcacc tacccctccg gcagcgacaa
     1021 ctacaagcgc atcatgctgc tgctcaaaga cctttacgtt caggccggcg tgctgatgga
     1081 gcccgacccg gtggactggc ctgtactgat caacacgctg aacaccaaga actttgacgc
     1141 ggtgagcctc gcgtggacat ccggattcga gatcgacatc taccagatgc tgcattcttc
     1201 gcagacagag cccggcgggg acaacttcat cagctacatc aacccggggc tggacgagct
     1261 catcgaaacc gcccgccagg agatggacga agaggcccgc atggccatct ggcatcaggt
     1321 gcaccgcgag atctgggccg agcagcctta cacctatctg ttccggacca agtcgctgat
     1381 cttcgcggat cggcgcatcc gcaacgtgca ggtggttcgc gccgggctca acgagggcgg
     1441 actgtggaac atgcccatgg aatggtacgt gcccaccgac gcgcagaagt acagcgactg
     1501 acggcaggct gagaaggaag cagagcgcaa cacgatggtc aactacgtca tccggcgcct
     1561 gctgctgata ttcccgaccc tgctgggcat caccatcctc gtcttcgccg tgatggcggc
     1621 tgcccccggt ggcgtgggcg ccagcctgct ctcccgctcc ggagatctgc gacccgagga
     1681 gcgcgccgcc atggagcgct acatcaacga gcgttacggc ctggacaagc cgatgcccat
     1741 gcagtacctg cgctggctca acaacgtctc gcccatcggc ttccgcaagg acgagaacaa
     1801 cgaggacgcc gggttcggct tcaaggtgcc cgacctcggc atgagcttcg ccaagaaccg
     1861 tccggtgact cagctggtag cccaggcgct gcccatcacg ctcctgctga acgcgctttc
     1921 cgtgcccctg gtgttcatcc tgtccatcgt gatgggcgtc tacacggcac gtttccgggg
     1981 gcaagccctc gacgtgggca tcggcaccgc caacctggcg ctgtggtcca ttcccaccat
     2041 ctggatgggc gtgctgctca tcggcctgct ggccagcgag gagtacgcgc gcatctttcc
     2101 cacatccggc ctcgagagtc tgcgcgccga ccgctggacg ttcctgccgg tggtcaccga
     2161 gcagggctgg cagcgcggct acctgctgga cgttgtctgg cacctggtgc tgccgatcgt
     2221 ctgcctgacc tacaccggct tcgccgtgct caccaagctg acccgctcgg cggtgctgga
     2281 gaacctgcag gcagactacg cgcgcaccgc ccgggccaag ggcgcctcgg agagaacggt
     2341 gctctggtat cacgtcttcc gcaacagcct gctgccgctg ctgaccgtag ccgcctccat
     2401 cctgccgggc atgctggtgg ggtccgtcat cgtggagagc atcttctcca tagacggcat
     2461 gggccgtctc gtggtcgagg ccgtgcagct gcgggaccgg gaagtagtgc tgtcgatcac
     2521 cctcatcacc agcctgctca cgctgctgtc ctacctcatc gtcgacatct gctacgccgc
     2581 ggcggatccg agggtcagct tcgaatgacc gagaccgcca tcaccagagc tgttgagaag
     2641 gtgggagagg aatcgttctc ccgccgcgtg acccgggaca ccctgcgcaa cacgggcgcg
     2701 aagataggga tcgcctgggt cgccacggtg gccgttctgg cggtattcgc gccgttcatc
     2761 gccaactcgc acccctatct gatgcgcgtg gaaggcatgt ggtccagccc cctgctcgcc
     2821 cacctgagct gggcagacgt caccatgctc gtcaccttct tggccgtcgt cgccgcctgg
     2881 cgcctgcgcc gccgattcgg gcccggccag ctgctcgtcg gcgtcggggt gatcagcctg
     2941 gtgacgctgc tggctgccca gcagctcgtc caggtgcccc gtgcggtgat ctacgaccag
     3001 taccgggtcg gcctggccga aggcgggatc gaaaccgcct acttcgcacc cgtgccgttc
     3061 tcgccaaccg accggtcccg ggacaacccc gcccccctgc acgaaccgga cagcgtgcac
     3121 tggttcggca ccgacgagga cggcgccgac gtgctcagcc ggatgattca cgcctgccgc
     3181 atcgcccttg gcatcggcct ggtggccacc ggcatcgcgc tcgccctcgg catcgtttac
     3241 ggcggcatca tgggcttctt cagcggctgg ctggacatcg ccatgatgcg ctttctcgaa
     3301 atattcgagt ccatcccgtc actgttcctg ctgcttacca tcgtggcgtt cttcggccgc
     3361 aacatctatc tgatcatggt ggtcatcggc ctgacgtcgt ggcccggcta cgcccgcttc
     3421 ctgcgtgccg agttcctgaa gctgcgcaac caggactacg tgcaggcggc g
//
LOCUS       DBNHQR010000551         2760 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1382802, whole genome shotgun sequence.
ACCESSION   DBNHQR010000551 DBNHQR010000000
VERSION     DBNHQR010000551.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1382802"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..942
                     /locus_tag="AC16VR_12580"
     CDS             52..942
                     /locus_tag="AC16VR_12580"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR6012038.1"
                     /translation="MQRKDHIDTLAAVTLIVMNVLLGLNQSVVKLVNDGFAPVFQSGL
                     RSACAILPVLGYALVMRKRLSVTDGSLGLGMLNGLLFSGEFCLLFLALDYTSVARVSL
                     FFYVMPVWVTIGAHFLVPGERMTLLKAGGLVLALAGVALGLGIGSGSAGEQAWIGDLL
                     ALGGAIFWASIALLTRTTRLQRCSPEMNLLYQLAVSAVILLAVAPLFGDTVREPTPAI
                     LGMFAFQVIVIVAIGFVVWFWILSIYPVSHMASFSLLAPVFGVLFGWLIFDDQITPAF
                     VIALLLVGSGIVLINRSPAG"
     gene            947..1957
                     /locus_tag="AC16VR_12585"
     CDS             947..1957
                     /locus_tag="AC16VR_12585"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIR6012039.1"
                     /translation="MDNGRWTVASRPEDQVEEANFQFVSESVGEPEDGEFLIGTRYLV
                     VSPPARMALTSGGIARRPVPIGATMRGTGLGQVVASRHPGFAEGELVIGDLGWQEYAI
                     SDGATRHPVRKVAARPGMPESTLLHVLGAGGATAYFGMIEYAKPHVGDTLVVSAAAGN
                     VGSLVCQLGRLQGCRVVGIAGSDEKCSWLTNDLGCAAAINYKREDVAAALDGACPDGI
                     DVYFDNVGGEILDAALARIAQGARVVLCGGTSQYNHDLDWYGPKNYFNLVYRQAEMAG
                     FYVYNFSHRFEEAFGRLESLLATGELKYAEDVLQGIGQTPAALIRVLKGENFGVQLVR
                     VA"
     gene            complement(1944..>2760)
                     /locus_tag="AC16VR_12590"
     CDS             complement(1944..>2760)
                     /locus_tag="AC16VR_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012040.1"
                     /translation="QGFLSAVLEDGNLLVERLDIRPPSGRVQGRLAVRPEDESGTIDL
                     ELRGDRVALNVAANLEPSAEPADQLVFDVDVYLRARGTDARALAASLEGEVSALSDGG
                     RIHNRQLALIFGGFGRELLGNINPFIKHDPYTDLSCVVFRFSATDGILKADPYLAIRS
                     DKLNLVSSGFANLATERIDFTFNALPRLRLSISATELINPYVRVSGTLLKPTLSLDSR
                     SAAVTGGAAALTGGLSLLAQATWKRTFGSRDACGKALEELRAEEGAAAAVTPP"
BASE COUNT          431 a          864 c          923 g          542 t
ORIGIN      
        1 cccgggtttc gttgataccc attcggttca ctgcggctat catgctgccc catgcagcgc
       61 aaagaccaca tagacactct ggcggccgta acgctcatcg tcatgaacgt cctgctggga
      121 ctgaaccagt ccgtggtcaa gcttgtcaac gatggatttg ctcccgtgtt tcagtcggga
      181 ttgcgctccg cgtgtgccat cctgcccgtt ctcggctacg cgctcgtgat gcgcaagcgt
      241 ctctcggtca ccgatggcag cctgggcctg gggatgctca acggcctgct tttctccggc
      301 gagttctgcc tgctcttcct cgctttggac tacaccagcg tggccagggt ctccctgttt
      361 ttctacgtca tgccggtatg ggtgaccatc ggcgcccact ttctcgttcc gggcgagcgg
      421 atgacgctgc tcaaggccgg cggccttgtc ctcgcgctgg ccggcgtagc cctgggtctt
      481 ggcatcggct ccggctcggc cggcgagcag gcctggatcg gagacctgct ggcgctggga
      541 ggcgcgatct tctgggcgag catagcgctg ctcacccgta ccacccggct gcagcgatgc
      601 tcgccggaga tgaacctgct ctaccagctc gccgtatccg ccgtgattct gctggcggtg
      661 gcgcctctgt tcggcgacac ggtgcgtgag cccacaccgg ccatcctcgg gatgtttgcg
      721 tttcaggtca tcgtgatcgt cgccatcggc ttcgtggtct ggttctggat cctgtcgata
      781 tatcccgtct cccacatggc ttccttcagc ctgctggcgc cggtgttcgg ggtcttgttc
      841 ggatggctga tcttcgatga tcagattacg ccagcgttcg tgatcgcctt gctgctggtt
      901 ggatctggta tcgtgctgat caaccgcagc ccggcaggat gagtggatgg acaacggcag
      961 atggacggtc gcgtcgcgcc cggaggacca ggtggaagag gccaatttcc agttcgtcag
     1021 cgagtcggtg ggcgagccgg aggacggcga gtttctcatc ggcacgcgct atctggtggt
     1081 gagcccgccg gcccggatgg cgctcacctc tggcggcatc gcgcgccgtc cagtgccgat
     1141 cggggccacc atgcgcggaa cggggctggg ccaggtggtc gcctcccgcc atccggggtt
     1201 tgccgagggc gaactcgtca tcggggatct cggctggcag gagtacgcca tctcggacgg
     1261 cgccacgcgc caccccgtgc ggaaggtggc agccagaccg gggatgccgg aaagcaccct
     1321 gctgcatgtg ctgggggccg ggggtgcaac cgcctacttc ggcatgatcg agtatgccaa
     1381 gccgcacgtg ggcgacacgc tggtggtctc ggcggcggcg ggcaacgtcg gctcgctggt
     1441 ctgtcagctg ggccggctgc agggctgccg ggtagtcggg atagcaggca gcgacgagaa
     1501 gtgttcctgg ctcaccaacg atctaggctg cgcggcggcg atcaactaca aacgtgaaga
     1561 cgtcgccgca gccctggacg gagcctgccc tgacggcata gacgtctact tcgacaacgt
     1621 cgggggagag atcctcgacg ctgcgcttgc gcgcattgcc cagggcgcca gggtggtgct
     1681 gtgtggcgga acgtctcagt acaaccacga cctggactgg tacggaccga agaactactt
     1741 caatctggtc tatcgccagg ctgaaatggc cggattctac gtctacaact tctcccaccg
     1801 cttcgaggag gcctttgggc ggctggagtc cctgctcgcg acgggagagc tcaagtatgc
     1861 cgaggacgtg ctgcagggaa tcgggcagac gccggcagcg ctgattcgcg tcctcaaagg
     1921 cgagaacttc ggcgtgcagc tggtcagggt ggcgtgacgg ctgctgctgc gccctcttcc
     1981 gcgcgcagtt cctccagggc cttgccgcag gcgtcgcggg agccgaaagt gcgcttccag
     2041 gtggcctgag cgagcaggga caaacctccg gtcaatgccg ccgcgccgcc cgtcaccgcc
     2101 gcgcttcggc tgtccaggct caaggtcggc ttaagcaggg ttccactcac ccgcacgtag
     2161 gggttgatca gctcggtggc gctgatactc agtcggagtc ttggcagcgc gttgaaggtg
     2221 aaatcgatgc gctcggtggc caggttggcg aatccgctgc tgaccaggtt cagcttgtcg
     2281 gagcgtatcg cgaggtacgg atcggctttc agaatgccgt ccgttgcgga gaatcgaaac
     2341 accacacagg acaaatccgt gtaaggatca tgtttgatga aaggattgat gttgccgagc
     2401 agctcgcgcc cgaacccgcc gaagatcagc gcgagctgtc ggttgtggat ccggcccccg
     2461 tcgctcagcg cgctgacctc gccttccagc gatgctgcca gagcccgcgc atcggtgcct
     2521 cgggcgcgca gatacacgtc gacgtcgaac acgagctggt cggcaggctc cgcggacggt
     2581 tcgaggtttg ccgccacgtt cagcgccacc cgatcaccgc ggagctcgag gtcgatggtg
     2641 ccggattcgt cttccggccg gaccgctaga cggccttgca cccggccgct gggtgggcgg
     2701 atgtcgaggc gttccaccag caagttgccg tcctccagca cggcggacag gaacccctgg
//
LOCUS       DBNHQR010000552         3733 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_371655, whole genome shotgun sequence.
ACCESSION   DBNHQR010000552 DBNHQR010000000
VERSION     DBNHQR010000552.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_371655"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="AC16VR_12595"
     CDS             <1..209
                     /locus_tag="AC16VR_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014577884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HIR6012041.1"
                     /translation="ASVDGLVIAGFTTSGCVRASAVDALQNDYRTTVIEDAVGDRDPA
                     AHAANLYDIEAKYGDVRSLVSLLA"
     gene            complement(214..1923)
                     /locus_tag="AC16VR_12600"
     CDS             complement(214..1923)
                     /locus_tag="AC16VR_12600"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl-D-amino-acid deacylase family protein"
                     /protein_id="HIR6012042.1"
                     /translation="MLDLIIRNGRIIDGTGAPAFSGDLGIVGGRIAAVGSVADDARRE
                     IDAAGRVVAPGFVDVHTHYDAQVFWDGTLSPSCYHGVTTIFGGHCGFSIAPLSTEAAP
                     YLLHMLARVEGMPVASLEAGVPWDWQSFAEYLGKLDGRLGINAGFMAGHSAIRRVVMG
                     ERAVGHQASDAEIRSMADLLDRCLAEGAMGFSSTLSPTHNDADGQPVPSRHASREELI
                     ELARVCGLHEGTSIEFLPEVGTFSEETKQLMSDLSTTAGRPVNWNVLTPGNPEVVASQ
                     LDASDVARRNGGKVVALTVPQPVLLRINLYGGMVFDAMPGWAPLFELPVPERIEALKD
                     PARRRALDASANSGGPFKELAEWRKVLVHATFRPENKQLEGRTIGSIADEQGKEPIDA
                     LFDLAISEGLRTSFMPPAFAGSGDESLWAVRGELWQDERTVIGASDAGAHLDMIDTFA
                     FSTQVLGNGVRKYGVISLEEAVHQITQVPAELFGLRERGRLESGWHADVVVFDPETVA
                     TGPTHVRSDLPAGEPRVYADAVGVDHVIVNGVEIVRDGTHTGALPGTTLRSGLDTQTP
                     RMD"
     gene            2013..3356
                     /locus_tag="AC16VR_12605"
     CDS             2013..3356
                     /locus_tag="AC16VR_12605"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spinster family MFS transporter"
                     /protein_id="HIR6012043.1"
                     /translation="MQSANDDAAEWPSAARGWYATIILLIAFTFSFVDRQVLNLLVEP
                     IRDHFGISDTEVSILQGFAFAVTYVLLSVPVGRMVDRFNRVAIMIGGVLVWSATTISC
                     GLSRTYTQILVSRLGVGAGEASLAPAAWSVLSDYFRPERLSRPISVYLMGPYLGAGIA
                     MIAGAQVLDWTRDVDFVEVPLLGQLAPWQLTFIAVGLPGVLIAALVGTVREPARHGRK
                     DAELIAPPWRDVLGYLWRHRGIYTSLHLGVPCIVVMLYGLQAWTPTVLVRVYEWDLAQ
                     AGRVYGFIALFAGSAGVLSGPTVARVLERRGYPDYPLRLAVIAAGCATLSMVALPWQS
                     SAEGALVCIALASFFVTLPLALMTSTMQFVTPNEMRGVVAGMYVVTTNVLGLGLGPTL
                     VAASTDFIFADTSALAYSMALVSVVMGPMAVVLLSVGMKPYITRAAELGAQGGKP"
     gene            complement(3371..>3733)
                     /locus_tag="AC16VR_12610"
     CDS             complement(3371..>3733)
                     /locus_tag="AC16VR_12610"
                     /inference="COORDINATES: protein motif:HMM:NF012398.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR6012044.1"
                     /translation="YYEPTLLADVHNGMTVAREEIFGPVTAVIPYEGIDEAVQIANDS
                     PFGLAASVYTRDQDLAMQVARQLRSGSVAVNAAGVSLTQPFGGYKQSGWGRECGPEGI
                     LEFTQIKQIVLGASYLDG"
BASE COUNT          555 a         1237 c         1241 g          700 t
ORIGIN      
        1 aggcttcggt ggacggcctt gtcatagcgg gattcaccac cagcggttgc gtgcgcgcct
       61 cggcggtgga cgctctgcag aacgactatc gaacgacggt gattgaagac gccgtgggcg
      121 accgggaccc cgccgcccac gcggccaacc tctacgacat cgaggcgaag tacggcgacg
      181 ttcgctctct ggtcagcctg ctggcctgaa cgatcagtcc attcgcggcg tctgggtatc
      241 cagacccgat ctcagggtgg tgccgggcaa ggcgccggta tgggtcccat cccggacgat
      301 ctcgacaccg ttcacgatca cgtgatccac gcccacagcg tcggcataga cccggggctc
      361 tccggcgggc aggtccgatc gcacgtgcgt tgggccagtg gccacggttt ccgggtcgaa
      421 cacgaccacg tccgcatgcc agccgctctc caggcggccg cgctcccgca gtccgaacag
      481 ctctgccggc acctgggtga tctggtggac ggcttcttcc agagagatca cgccgtattt
      541 gcgaacgccg ttgccgagca cctgggtcga aaaggcaaag gtgtcgatca tgtccagatg
      601 ggcgcccgcg tccgaggcgc cgatgaccgt tcgctcatcc tgccagagct cgccgcggac
      661 ggcccacagg gactcgtcgc cgctgcccgc gaaagcgggg ggcatgaacg aggtccgcag
      721 gccctcggag atggcgaggt cgaagagggc gtcgatgggc tctttgccct gctcgtcggc
      781 aatcgagccg atggtgcggc cctctaattg tttgttctcg ggtctgaacg tggcgtgcac
      841 gagcactttg cgccactcgg ccagctcctt gaaggggccg ccggagttgg cgctggcatc
      901 gagcgcgcgg cgccgggcgg gatccttcag cgcttcgatg cgctcgggaa cgggcagctc
      961 gaacagcggc gcccagccgg gcatcgcgtc gaacaccatg ccgccgtaga ggttgattct
     1021 gagcagcacc ggctggggaa ccgtgagcgc caccaccttg cctccgttgc gtctggcgac
     1081 gtcgctggcg tcgagctggc ttgccacgac ttccggattg cccggtgtga gcacgttcca
     1141 gttgaccggg cggcctgcgg ttgtcgaaag gtccgacatc agctgcttgg tttcctcgga
     1201 gaacgtccct acctcgggga gaaactcgat gctggtcccc tcgtgcaggc cgcacacgcg
     1261 cgccagctcg atgagctctt cgcgactggc gtgccgggag ggaacgggct gaccgtccgc
     1321 gtcgttgtgg gttggggaga gcgtggagga gaagcccatg gcaccttcag ccagacagcg
     1381 atccagcagg tcggccatgc tccggatctc tgcgtcgctg gcttgatggc cgaccgcgcg
     1441 ctcacccatg acgacccggc ggatggcgct gtggccggcc atgaaacccg cgttgatccc
     1501 cagccgccca tcgagcttgc cgagatactc ggcaaagctc tgccagtccc agggcactcc
     1561 ggcctccagg ctcgccaccg gcattccttc cacgcgggcc agcatgtgga gcaggtaggg
     1621 ggccgcctcg gtgctcagcg gcgcgatgct gaaaccgcaa tggccgccga atatggtggt
     1681 cacgccgtgg taacacgacg ggctcagcgt gccgtcccag aagacctggg catcgtaatg
     1741 agtgtgcacg tccacgaagc cgggggcgac cacccgaccc gccgcgtcta tttcccgccg
     1801 cgcgtcgtcg gcgaccgagc caaccgcggc gatgcgtccg ccgactatgc cgagatcgcc
     1861 gctgaacgcc ggcgcgccgg tgccgtcgat gatgcggccg ttcctgatga tcagatcgag
     1921 catctcgctc ctcccgttgt tagcccgtaa gatatagctt tccggcgcgt taccaaagcc
     1981 cggtgatcag ctcaagcccg ggaggggccc ctttgcagtc tgccaatgac gacgcggccg
     2041 aatggccgtc cgccgcgcgg ggatggtacg ccaccatcat cctgctcatc gccttcactt
     2101 tctctttcgt ggatcggcag gtactgaacc tcctggtcga gcccattcgg gatcacttcg
     2161 ggatctccga tacggaagtc agcatcctcc agggctttgc gttcgcagtc acctacgtgc
     2221 ttctgagcgt tcccgtgggg cgcatggtgg atcggttcaa ccgcgtggcc atcatgatcg
     2281 gcggcgtgct ggtctggagc gcgaccacca tctcctgcgg gctgtcgcgc acctacaccc
     2341 agatactcgt ctcgcgcctg ggcgtggggg ctggcgaggc ttcgctggcg cccgcggcat
     2401 ggtccgtgct gtcggactac tttcgcccgg agcgtctttc gcgccccatc agcgtctacc
     2461 tgatgggtcc gtacctcggc gccggtattg ccatgatcgc aggcgcacag gtgctcgact
     2521 ggacccgcga cgtcgacttc gtggaggtgc ctttgctggg ccagctggcg ccatggcagc
     2581 tgaccttcat cgcggtaggg ctgcccggcg tgctcatcgc ggcgctggtc ggcacggtgc
     2641 gtgaaccagc ccgtcatggt cgcaaggacg ccgagctcat cgccccgccc tggcgggacg
     2701 tgcttggcta cctgtggcga cacagaggca tctacacctc cctgcatctc ggggtgccct
     2761 gcatcgtggt gatgctttac gggctgcagg cgtggacgcc cacggtgctt gtgagggtct
     2821 acgagtggga tctggctcag gcggggcggg tctacggctt catcgcgctc tttgccggtt
     2881 ccgcgggggt gctgtcgggg cccaccgtgg cacgtgttct ggaacggcgc ggataccccg
     2941 actacccgct gcggcttgcc gtgatcgctg ccggctgcgc gacgctgagc atggtcgcgc
     3001 tgccctggca gtcaagcgcg gagggtgcgc tggtctgcat cgcgcttgcg agcttcttcg
     3061 tcactctgcc tctggcgctc atgacgtcca ctatgcagtt cgtgacgccc aacgagatgc
     3121 gcggcgtcgt cgcgggcatg tacgttgtta ccaccaacgt gctcggtctg gggctcgggc
     3181 ccacgctcgt cgccgcttcc accgatttca tctttgcaga cacctcggcg ctggcctatt
     3241 ccatggcgct ggtctctgtc gtcatggggc ctatggcggt ggtgctgctg agcgtcggca
     3301 tgaagcccta catcacccgt gcggcggagc tcggcgcgca gggagggaag ccgtaggccg
     3361 gcctgccaga tcagccgtcc agatagctgg cgccgaggac gatctgcttg atctgggtga
     3421 actccagaat tccttcgggt ccacactcgc ggccccatcc cgactgcttg taccctccga
     3481 atggctgggt cagcgagacg ccggcggcgt tcacggccac gctgccgctg cgcagctggc
     3541 gcgccacctg cattgccaga tcctggtcgc gggtatagac cgacgccgcg agcccgaaag
     3601 gtgagtcgtt ggcgatctgc accgcttcgt cgatgccctc gtagggaatg accgcggtta
     3661 ccgggccgaa gatctcttcc ctggccaccg tcatgccgtt gtggacgtcg gccagcaggg
     3721 tcggctcgta gta
//
LOCUS       DBNHQR010000553         2065 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_561893, whole genome shotgun sequence.
ACCESSION   DBNHQR010000553 DBNHQR010000000
VERSION     DBNHQR010000553.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_561893"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /gene="ccmI"
                     /locus_tag="AC16VR_12615"
     CDS             complement(<1..1003)
                     /gene="ccmI"
                     /locus_tag="AC16VR_12615"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HIR6012045.1"
                     /translation="MSFWLMAGLMMLAGVLIVVVPLLRGGLQGGRTDRSVAVRSLYQE
                     RLAELDVDVAAGQLSPASRDAVAEELGSALLGDFEQTASGQGVDSRPVSPAVVLTLAV
                     LLPLVVLIVYLEVGDPGAQQIAGAEALLAMDPRTQRAEVAGWRDRLDSRVERRSDDAS
                     SWYLLGLANLQLGSFGRAAQAFAMSHALSGESPVVDAYWLQARYLSSGGEIDRETRVI
                     GDRLLKAQPNQPLVLEIYAIDAYRRGDYREAVALFSRALSGNLETEQRAVLAAGLQQA
                     RARLGDLVPSLDVSVTADGASPAGWTLFVIARPPGGGMPYAVVRRPSTSLPLDLRLDD
                     "
     gene            complement(1000..1464)
                     /locus_tag="AC16VR_12620"
     CDS             complement(1000..1464)
                     /locus_tag="AC16VR_12620"
                     /inference="COORDINATES: protein motif:HMM:NF015853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HIR6012046.1"
                     /translation="MRMADRRRAAIFGLLVAAGAALGAGPVDVYDFPDPATEARYRAL
                     IAEFRCPKCLNTNLAGSDAPIAKDLRRTVHRLVVVEGMSDAEVREFLQSRYGDFVLYD
                     PPFRFDTSILWLAPGLLLLVGLGVLYRVLRQPPPPKLSETDARRLREILEES"
     gene            complement(1451..1990)
                     /locus_tag="AC16VR_12625"
     CDS             complement(1451..1990)
                     /locus_tag="AC16VR_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HIR6012047.1"
                     /translation="MRRLRLFVPLLVFVIIVGFGYAGFKLNDPRELPSALLDRPFPEF
                     SASLLGGAGTVSRDALLGSPVLVNVWATWCPTCRAEHDELMRIRAETGLRLVGVNYKD
                     DPVKALRWLADFGDPYEFTLQDADGSLGVELGVYGAPESFLLDARGVIVYKRVGEVNE
                     RVWKEEILPALERLHDENG"
BASE COUNT          392 a          730 c          660 g          283 t
ORIGIN      
        1 cgtcgtccaa ccgcaggtcc aggggcagag atgtcgatgg tctgcggacc accgcgtacg
       61 gcatcccgcc tcccggcggg cgggcaatga cgaacagcgt ccagcccgcg ggcgaggcgc
      121 cgtccgcggt cacggacacg tccaggctcg gaaccaggtc tccgaggcgg gctcgcgcct
      181 gctgcagccc ggctgcgagc acggcgcgct gttcggtctc caggtttccc gagagagccc
      241 gcgagaacag agccactgcc tcccgatagt cgccgcgccg ataggcgtcg atggcgtaaa
      301 tctcgagcac cagcggctgg ttgggctgcg ctttcagcag acgatcgccg atgaccctgg
      361 tttcccggtc aatttcgccc ccggagctca ggtaccgagc ctggagccag taggcgtcga
      421 ccaccgggct ttcgcccgac agcgcgtggg acatggcaaa cgcctgcgcc gcccgcccga
      481 agctgcccag ctgaagattc gcaaggccca ggagatacca gctgctggca tcgtccgagc
      541 gccgctcaac gcggctatcg agccggtctc gccaaccggc gacctcggcg cgctgggtgc
      601 gcgggtccat ggcgagaagc gcttccgcgc ccgctatctg ctgcgcgccc ggatctccga
      661 cctcgagata gacgatcagc accaccaggg gcaacagcac cgcgagcgtc agcacgacgg
      721 cgggcgacac cggtctggaa tcgacgccct gcccggacgc cgtctgctcg aaatcgccga
      781 gcagagcaga gcccagctcc tcggccaccg cgtcgcggga tgctggcgag agctgaccgg
      841 ccgccacgtc gacgtccagc tcggcgagcc gctcctggta gagggatcgg accgcaacgc
      901 tcctgtccgt ccggccgccc tgaagccctc cgcgcagcag cgggacaacc acgatgagca
      961 cacctgcgag catcatcagc cccgccatca gccagaagct catgattctt ccaggatctc
     1021 acgcagccgc cgggcgtctg tctcgctgag cttcgggggc ggcggctgcc ggaggacgcg
     1081 atacagaacc ccaaggccta cgagcaacag gaggccggga gccagccaga ggatcgacgt
     1141 gtcgaaccga aagggcgggt cgtaaagcac gaaatccccg taacgcgact ggagaaactc
     1201 gcgaacttcg gcgtcgctca tgccctcgac gacgacgagc cgatgcaccg tgcgccgcag
     1261 gtccttggca atgggcgcat cggagccggc gaggttggtg ttcagacact tcggacagcg
     1321 aaactccgca atcagcgcac gatagcgcgc ttccgtagcc gggtcgggaa agtcgtagac
     1381 gtccaccggg ccggccccca gggccgcacc cgcagcaacg agcaggccga aaatggccgc
     1441 gcgacgacga tcagccattc tcatcgtgca ggcgctccag cgcgggcagg atttcttctt
     1501 tccagacgcg ctcgttcacc tccccgaccc gcttgtagac gatcaccccc ctggcatcga
     1561 ggagaaagct ttccggagcc ccgtagacgc cgagctccac ccccagggat ccgtccgcgt
     1621 cctgaagcgt aaattcgtat gggtccccga aatcggcgag ccagcgcagc gccttgaccg
     1681 ggtcgtcctt gtagttcacc cccacgaggc ggagccccgt ctcggcgcga atgcgcatca
     1741 gctcgtcgtg ctcggcccgg caggtgggac accaggttgc ccatacgttg accagcaccg
     1801 gggagcccag cagagcatcc cggcttacgg tgcccgctcc cccgagaagc gaagccgaga
     1861 actctggaaa cggacgatcg agcagcgccg aggggagctc gcgaggatca ttgagcttga
     1921 agcccgcata gccgaaaccg acgatgatga cgaacaccag cagcggcacg aacaggcgca
     1981 gccgtctcat cgcgccgcct ccgcgacctg agccgacagg agcttctcct tgagccgccg
     2041 gtagcgcgaa tcgaaaaccg ccagg
//
LOCUS       DBNHQR010000554         6248 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1638619, whole genome shotgun sequence.
ACCESSION   DBNHQR010000554 DBNHQR010000000
VERSION     DBNHQR010000554.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6248)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6248)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6248
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1638619"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..158)
                     /locus_tag="AC16VR_12630"
     CDS             complement(<1..158)
                     /locus_tag="AC16VR_12630"
                     /inference="COORDINATES: protein motif:HMM:NF023178.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HIR6012048.1"
                     /translation="MPEILFLLVAGHAICDFVLQSEAMATGKNHRLKEKMRERHGPSF
                     PSWPYWLTS"
     gene            356..784
                     /locus_tag="AC16VR_12635"
     CDS             356..784
                     /locus_tag="AC16VR_12635"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6012049.1"
                     /translation="MIELDEEIKGRLDRAIEEHKTVTVAYVDVDGKPHISFYGSTHVH
                     SSNQLAIWVRNPQNDLIRTIPDRPNVAIIYGDIGGRVYYTFEGTASVTDDAEEQERVY
                     QEMHPVERQFDPDRGGVAVVVDLDRVTLLSAAAGKQVMER"
     gene            complement(810..2018)
                     /locus_tag="AC16VR_12640"
     CDS             complement(810..2018)
                     /locus_tag="AC16VR_12640"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6012050.1"
                     /translation="MAELSLESIFTPEIIADPYPLYRQLRETSPVLELPDANMVILTR
                     YADVQTVLRDRRLGHADESMLTRGDLEQARNNPAIANLRRTMLLKNPPDHTRLRGLVV
                     KAFDARRVEAMRTRIRAIANELVDAFVDAGSGDLVRLFTHPLPVIVICDLLGVPKADQ
                     AEFVQGTRISGRLIDPSPMTEEELAQANASTTESQRYFEQLCDERRQAPRDDLITALV
                     QSETEHGKLSKEELTSNISLLFAAGHETTVNLMGNGLIALYRNRDQLDRLRADPSLMP
                     NAVEEFLRYDSSVQLTGRDALEDAEIAGIALPRGRAVLALLAAANRDPEAFDRPEELD
                     IARKRIKPVSFGGGIHLCLGAQLARIEAHEALNVLLERLPNLELDDVATPDWKQTITL
                     RGVRTLPATW"
     gene            complement(2088..3041)
                     /gene="cysK"
                     /locus_tag="AC16VR_12645"
     CDS             complement(2088..3041)
                     /gene="cysK"
                     /locus_tag="AC16VR_12645"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015371271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019344 - L-cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HIR6012051.1"
                     /translation="MIYDNILQTIGNTPIVRLNRTGPDHVEMYVKVEAFNPLASVKDR
                     LAYAIIKDATDSGALQPGQTVVEATSGNTGIALAMVCAATGHPFVATMVETFSIERRK
                     IMRALGAKVILTPAAERGSGMVRRAEELAAEHGWFLARQFENPANPAYHRNTTGPEIL
                     KDFAGRRLDYWVTGWGTGGTLTGAGEVIKVARPDVKIIAAEPEQASMLAGKEWSPHMI
                     QGWTPDFIPAVLNPEVCDAIVPVNQDVAKDTALALARNEGIFVGVSAGGTLAAALDVA
                     EDAAPGSVFVVMLPDTGERYLSTFMFEGINEGSDDDWLESL"
     gene            complement(3158..3712)
                     /locus_tag="AC16VR_12650"
     CDS             complement(3158..3712)
                     /locus_tag="AC16VR_12650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR6012052.1"
                     /translation="MTQPARETLAAMLQVHLGRLRRLALVLTENPDDAEDLVQDVVVK
                     LCGRPHEVAGVRDLSTWLNRVVYNQFIDDRRRRKRQPLQLVGSADELDAAMPGESAEP
                     MLEALCKDRDQRLGDAVNALNAEQRMVLLLHDSQGYRLTEIHQLTDIPLGTLKSRLHR
                     ARSRMRVLLDSERVPDDRSEPDQG"
     gene            complement(3877..4224)
                     /locus_tag="AC16VR_12655"
     CDS             complement(3877..4224)
                     /locus_tag="AC16VR_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012053.1"
                     /translation="MSTTNPGNDPHKDPLDALLSEAFQQDIEQVGDADMAGRVMRTLK
                     RRTRLRAAILGVSMAAGLSLATALALPGLTDLLRLLAEQAPVEPSSWGVLPIAALLAI
                     LTPWLFSLVDDRV"
     gene            complement(4221..4772)
                     /locus_tag="AC16VR_12660"
     CDS             complement(4221..4772)
                     /locus_tag="AC16VR_12660"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR6012054.1"
                     /translation="MEVSDETLVQQVVRLKDLQAFERLVQRHEKRILHLQRRFIRDHA
                     LAEDLCQETFLRAWDRLETFSGRGSFAAWLTKLAYNIFLQHRRKHAARTSRETPYEEE
                     AEAGQTALEGPAPNADELPDLPRLLAILPEADRLVLLLSYGHGLSIAEIADVLETPDG
                     TIKSQIHRAKDKIRRHFSIGAAA"
     gene            complement(4791..5153)
                     /locus_tag="AC16VR_12665"
     CDS             complement(4791..5153)
                     /locus_tag="AC16VR_12665"
                     /inference="COORDINATES: protein motif:HMM:NF039701.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HIR6012055.1"
                     /translation="MEDILVPISLFLVIGAVISLSFYFRYRTRQEIQSTVRAAIERGQ
                     ELTPEVLDGLSDALNSRHGDLRRGIISLAIGIALFLFAGLLGEPDAEGPLMAISAFPL
                     LLGIGYLAMWFFLRRKAG"
     gene            5374..5949
                     /locus_tag="AC16VR_12670"
     CDS             5374..5949
                     /locus_tag="AC16VR_12670"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013315.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6012056.1"
                     /translation="MADQDRKKWDQRYAAGAYADRPHPSAILADWLPRLSAGDALDIA
                     CGAGRNALHMARAGFRVDAVDVSAEALARAASAAAEAGLEVNWMQRDLDTGLPVEGPY
                     DLIVMIRYVDESLLRALPALLRPGGVLIVEEHLTTEEPVIGPRDARFRVMPGALRAAV
                     PDLEVLEATDTVTDDPDGRRVALARLVARKP"
     gene            complement(5963..>6248)
                     /locus_tag="AC16VR_12675"
     CDS             complement(5963..>6248)
                     /locus_tag="AC16VR_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012057.1"
                     /translation="IVEYGDGWLPVPVPEFDERLALLNRLCEERGRDRSEIDVCLMTV
                     VDSADTLAGIRDKGVNRVILSLPTLGEDEALRVLDGYANVVEWAKEIGDG"
BASE COUNT         1031 a         1977 c         2129 g         1111 t
ORIGIN      
        1 gaggtcagcc agtaaggcca ggaggggaag gaggggccgt gccgctcccg catcttctcc
       61 ttgaggcggt ggttcttgcc ggtcgccatg gcttcggact gcaggacgaa atcgcagatg
      121 gcgtgtcctg caacgagaag aaacagtatc tcaggcattg cttgtccaat ccgtggacga
      181 ccgggccgcg gccggcaatc cgcatcgaaa gtatggtaag tttcgccccc ctcgcgaagc
      241 gctccagccc ccggtctgcc cgggcggcga cggcgcttcg gcgacgcagg ggtcgccctc
      301 gtggcaagcg agtatagacc aaagacgggc agccaaagcc ccaggaggag acgcgatgat
      361 cgagctggac gaggaaatca agggccggct ggaccgggcc atcgaggaac acaagaccgt
      421 cacggtggcc tacgtggacg tggacggcaa gccccacatc tccttctatg gaagcactca
      481 cgttcacagc agcaaccagc tggcgatctg ggtgcgcaac ccgcagaacg atctgatcag
      541 aacgattccc gaccggccca acgtcgccat catctacggt gacatcggcg gtcgcgtgta
      601 ctacaccttc gagggtaccg cctcggtgac ggacgacgct gaagagcagg agcgggtgta
      661 tcaggagatg caccccgtgg agcggcagtt cgacccggac cggggtggcg ttgccgtggt
      721 cgtcgatctc gaccgggtca ccctgctgag cgcggccgcc ggcaaacagg tcatggagcg
      781 ctaaaggggg ggacgctctc cgccgccgac taccaggttg ccggcagcgt gcgcacgccg
      841 cgcagggtga tcgtctgctt ccagtccggc gtggccacgt cgtccagctc tagattcggc
      901 agtctttcga gcaacacgtt cagggcctcg tgggcctcga ttcgcgccag ctgcgccccc
      961 agacacaggt ggatgcctcc gccgaacgac accggcttga tgcgcttccg ggctatgtcc
     1021 agctcctccg ggcggtcgaa cgcctcgggg tcgcggttgg cggctgccag cagcgccagg
     1081 acggcgcgcc cgcgcggcag ggcaataccg gcaatctcgg cgtcttccag cgcgtcccgg
     1141 cccgtcagct gcacggagct gtcgtaacgc aggaactcct cgacggcgtt gggcatgagc
     1201 gaggggtctg cgcgcagccg gtcgagctgg tcccggttcc ggtagagggc gatgagcccg
     1261 ttgcccatca ggttgacggt ggtctcgtgg ccggctgcga acagcaaaga gatgttcgac
     1321 gtgagctcct ccttgctgag cttgccgtgc tcggtctcgc tctgcacgag ggcggtgatc
     1381 aggtcgtccc tgggcgcctg gcggcgctcg tcgcacagct gctcgaagta acgctggctc
     1441 tccgtggtgc tcgcgtttgc ctgagcgagc tcttcctccg tcatggggct cgggtcgatg
     1501 agccgtccgg aaatgcgcgt gccctgcacg aactcggcct ggtctgcctt gggcacgccg
     1561 agcaggtcgc atatgacgat gaccggaaga ggatgggtga acaggcggac caggtcaccg
     1621 ctgccggcat cgacgaacgc gtccacgagc tcgttggcta tggctcgaat gcgcgtgcgc
     1681 atggcctcca cccgcctcgc gtcgaaagcc ttgacaacca gaccccgcag gcgggtgtgg
     1741 tcgggcgggt tcttgagcag catggtgcgc cgtaaattgg cgatggccgg gttgtttcgc
     1801 gcctgctcga gatcgccccg ggtcagcatg gactcgtcgg cgtggcccag gcggcgatcc
     1861 cgcaggaccg tctgcacgtc ggcgtagcgg gtgagaatca ccatgttggc gtcgggaagc
     1921 tcgagaaccg ggctcgtctc gcgaagctgc cggtatagcg ggtaggggtc cgcgatgatc
     1981 tcgggcgtga agatcgattc gaggctcaat tcagccattg ttactctccg ttcgcccgcc
     2041 gacctgccgg ttgtcagggc agctcggcgg catgactcgg cggccgatca cagggactcg
     2101 agccagtcgt cgtccgaacc ttcgttgatg ccctcgaaca tgaaggtgga aagatacctt
     2161 tctccggtgt ccggcagcat cacgacgaac acgctgccgg gcgccgcgtc ctcggcgacg
     2221 tccagggccg cggcgagcgt ccccccggcg gaaacgccga cgaagatgcc ctcgttgcgc
     2281 gccagcgcca gcgcggtgtc tttcgccacg tcctgattga cgggaacgat cgcatcgcac
     2341 acctcgggat tgagcaccgc ggggatgaag tcgggcgtcc atccctgaat catgtgcggg
     2401 ctccactcct tgcctgccag catggatgcc tgctctggct cggcggcgat gatcttcaca
     2461 tcggggcggg cgaccttgat cacttccccg gcgcccgtca gcgtgccgcc ggtgccccag
     2521 ccggtcaccc agtagtccag ccggcgcccg gcgaagtcct tcaggatctc ggggccggtg
     2581 gtgtttctgt ggtaggccgg attggccggg ttctcgaact gtcgcgccag gaaccagccg
     2641 tgctcggcag ccagctcctc cgctcgacgg accataccgc tgccgcgctc tgcggcgggt
     2701 gtgagaatga ccttcgcgcc cagggcgcgc atgatcttgc gccgctcgat ggagaacgtc
     2761 tccaccatgg tggcgacgaa cggatgtccc gtggccgcgc agaccatggc gagcgcgatg
     2821 cccgtgttgc ccgacgtggc ctcgaccacg gtctgtcccg gctgcagcgc gccggagtcc
     2881 gtggcgtcct tgatgatggc gtaggcgagg cggtccttca cggatgccag cgggttgaac
     2941 gcctcgacct tcacgtacat ctccacgtgg tcggggccgg tccgattgag cctgacgatc
     3001 ggcgtgttgc cgatggtctg caggatgttg tcgtaaatca tcgggatgcc actccgtgtg
     3061 cgaacttcgc tgcagcccac gataccccaa tggggtttgc ctcatccagg tgggcgtagc
     3121 cggtcggggc gtttcgagtt cttccggggc ggttcggtca gccctggtca ggttctgatc
     3181 ggtcgtccgg gaccctctcg ctgtccagca gcacgcgcat tcgggagcgg gcccgatgca
     3241 gcctggattt cagagtgccc agcggaatgt ccgtaagctg gtgaatctcg gtgagacggt
     3301 agccctgcga atcgtgcagg agcagcacca tcctctgctc ggcgttcaga gcgttcacgg
     3361 cgtcccccag gcgttggtcg cggtccttgc agagggcctc cagcatcggc tcggcggact
     3421 cgccgggcat ggcggcatcg agctcgtcgg ccgacccgac cagctgcagc ggctggcgct
     3481 ttcgccggcg ccggtcgtcg atgaactggt tgtagacaac gcggttgagc catgtggaaa
     3541 gatcccggac gccagcgact tcgtgcggcc gtccgcagag cttgactacc acgtcctgca
     3601 ccaggtcctc ggcgtcgtcc gggttttccg tgagaaccag ggccaggcgt cggagcctgc
     3661 ccagatgaac ctgcagcatg gctgcaaggg tttcccgagc gggttgagtc atgggaggtt
     3721 tcgtggtcct gatcaaactg cgatcgcgga tcagtgttgc acctcggacc ggcaaaggct
     3781 cgaacgtgtg cccacaatgg gtgcctggcg gaagggaaaa gtgtgcgcga gcccgcaaag
     3841 ttgagcgagc gcgccgtcgc gatgcgctcc ttcaggtcag acgcggtcgt ccaccaggga
     3901 gaagagccag ggcgtgagga tggccagaag cgcagcgatg ggcaggactc cccaggaaga
     3961 gggctcaacg ggggcctgct ctgcgagcag gcgcagcagg tcggtaaggc ccggcagggc
     4021 cagggctgtt gccaacgaaa gacccgctgc catgctcacc cccaggatgg cggcgcgcag
     4081 cctggtgcgc cgcttgagag ttcgcataac ccggccagcc atgtctgcat cgccgacctg
     4141 ctcgatgtcc tgttggaacg cctcgctgag cagggcgtcg agaggatcct tgtgaggatc
     4201 gttgccagga ttggtagtgc tcatgctgcg gcccctatgc tgaagtgcct gcgaatcttg
     4261 tcttttgccc ggtggatctg ggattttatc gtaccgtccg gcgtttcgag aacgtcggcg
     4321 atttctgcga tgctcagccc gtggccgtaa ctcagcagca gcacgagccg gtcggcctcg
     4381 ggaagtatcg ccagcagacg aggcaggtcc ggcaactcgt cggcatttgg cgccgggccc
     4441 tcgagcgccg tctgccctgc ctcagcctct tcctcgtatg gggtttcacg gctcgttctg
     4501 gcggcatgct ttctgcggtg ctgcagaaag atgttgtagg ccagcttggt gagccaggca
     4561 gcgaaactgc cccgcccgct gaaggtttcc agccggtccc acgcccggag aaacgtttcc
     4621 tggcagaggt cctccgccag ggcgtggtcc ctgatgaagc ggcgctgaag gtgcaggatg
     4681 cgcttctcgt gccgctggac caggcgctcg aacgcctgca gatccttcag ccgaacgacc
     4741 tgctgtacca gggtttcgtc gctgacctcc atcacgccat tagaccactg tcatccggct
     4801 ttgcggcgca ggaagaacca catcgcgaga tagccgatcc ccagcagcag cgggaaggca
     4861 gaaatggcca tcaggggacc ctccgcgtcc ggctcgccga gaagccctgc aaaaaggaaa
     4921 agcgcgatgc cgatggccag cgaaatgatt ccgcgacgca ggtcgccgtg gcgggagttc
     4981 agggcatcgg agaggccgtc gagcacctcg ggcgtcaact cctggccgcg ctcgatggcc
     5041 gcccggaccg tgctctggat ttcctggcgg gtccggtatc ggaagtaaaa ggacagtgag
     5101 atgacggctc ctatgacgag gaacagcgag atgggcacga gaatgtcttc cacgatctgt
     5161 ctccgtaatt ttctgtggct ctaactggac tgatgcacgt tttccgcttc cggttgcatg
     5221 ccgggagcga aaagtcggag acggtggggg gagatgaagg atgccccggg accgaggtgg
     5281 ggagagggga gggccggttc ccggggcgtc cagggtgaaa tgtgccccgg acgctgtgaa
     5341 aatcacgtga acgcccgatc acctggagag cccgtggcgg atcaggaccg gaagaagtgg
     5401 gatcagcgct atgcggccgg cgcttacgcc gatcgtccgc accccagcgc catcctcgcg
     5461 gattggctgc cccggctttc cgccggtgat gctctggaca tcgcctgcgg ggcgggccgc
     5521 aacgccctgc acatggctcg cgctggtttt cgggtcgatg ccgttgatgt ctccgcagag
     5581 gctctggcca gggcagcctc ggcggccgcc gaggccgggc tcgaggtgaa ctggatgcag
     5641 cgcgacctgg acaccggcct gcccgtggag gggccctacg acctcatcgt catgattcgc
     5701 tacgtcgacg agtcgctgct acgagcgctg ccggcattgc tgcgccctgg cggggtcctg
     5761 atcgtcgagg agcatctgac caccgaagag ccggtgattg gtccgcggga tgctcgcttt
     5821 cgggtgatgc cgggcgcgct gcgcgccgcg gtgcccgatc tcgaggtgct cgaggcaacg
     5881 gacacggtta ccgacgaccc cgatgggcgc agggtggccc tcgcccggct ggttgccaga
     5941 aagccctgag caaccggccg gctcagccgt cgccgatctc cttcgcccac tcaacgacgt
     6001 tggcatagcc gtccaggacg cgcagcgcct cgtcctcgcc cagcgtgggc agagacagga
     6061 ttacccggtt gacgcctttg tcccggatgc cggccagggt gtcggcgctg tccaccacgg
     6121 tcatcaggca gacgtcgatc tcgctgcggt cacggccgcg ttcctcgcag agccggttca
     6181 gcagagcaag gcgctcgtcg aactcgggta ccgggaccgg cagccagccg tcgccgtatt
     6241 ccacgatc
//
LOCUS       DBNHQR010000555         1106 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1510727, whole genome shotgun sequence.
ACCESSION   DBNHQR010000555 DBNHQR010000000
VERSION     DBNHQR010000555.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1510727"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..862)
                     /locus_tag="AC16VR_12680"
     CDS             complement(134..862)
                     /locus_tag="AC16VR_12680"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF025053.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR6012058.1"
                     /translation="MKIVAYSDTGKVRRNNEDAYGFDAEAGVAVLADGMGGLDAGEVA
                     SRIAVDTVLSGLRSSTGLSDALLAAVIAEANQAVLEKSIEKGVEMGTTLVTWLLTGQG
                     QCLVGHVGDSRAYRVRAGILRRLTRDHSMVQQMVDEGLLNEAEAETSPNRNVITRALG
                     IGGEVEVDVRSWVHSPGDLYLLCSDGLTDMVPEVEIQSILAAVGPPTEAQAADLDSAA
                     AALVDRANAAGGHDNVSVMLIRPC"
     gene            934..>1106
                     /gene="yaaA"
                     /locus_tag="AC16VR_12685"
     CDS             934..>1106
                     /gene="yaaA"
                     /locus_tag="AC16VR_12685"
                     /inference="COORDINATES: protein motif:HMM:NF015820.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HIR6012059.1"
                     /translation="MLAVISPAKRLDFTSEATTAKHSLPAFLKESKQLIQVLRKQSPA
                     ELAKLMGLSADLA"
BASE COUNT          202 a          384 c          311 g          209 t
ORIGIN      
        1 cgttcaccac ttccagatgc gcagggtcga accggtcggc aagcttccga ttgatggtgt
       61 cttgcgtggt caaggtgtct gcctgcaacg aaacggccga ttataccccg cgtcgcagca
      121 cgccgggatc gattcagcag gggcggatca gcatcacgga gacgttgtcg tggccgccgg
      181 cggcattggc ccggtcgacc aacgcggcgg ccgccgagtc gaggtctgcg gcttgtgcct
      241 cggtaggggg gccgaccgcc gccaggatgg actgaatctc cacctccggg accatgtcgg
      301 tcagcccgtc gctgcagagc agatagagat ctcccggcga gtgcacccag gagcgcacat
      361 ccacttccac ctcaccaccg atccccagcg cccgcgtgat cacgttccgg ttcggggacg
      421 tctcggcttc cgcctcgttc agcaggccct cgtcgaccat ctgctggacc atgctgtgat
      481 cccgggtcag cctgcgcaga attcccgcac gcacccgata ggctcgcgag tcacccacgt
      541 ggcccaccag acactgtccc tggccggtca gcagccaggt caccaaagtg gtgcccatct
      601 ccacgccttt ttcgatggac ttctcgagca ccgcctggtt ggcttcggca atcacagcgg
      661 cgagcagggc gtccgaaaga ccggtcgagc tccgcaaccc gctgagtacc gtgtctacgg
      721 cgatgcggct ggctacctca cccgcatcga gcccgcccat gccgtccgca agcacggcaa
      781 cacctgcctc ggcgtcgaac ccatacgcgt cctcgttgtt tctacgcacc ttgccggtat
      841 cgctgtacgc gactatcttc attctctctt tccggcagat ttcgttaaac tagccggcct
      901 tgccgttttt acttgagcta agagaaccgc tgaatgctgg ccgtcatatc gcccgccaag
      961 agactcgact tcacgagcga agccaccacg gccaaacata gcctgccagc tttcctcaag
     1021 gaatcaaaac agcttatcca agtgctccgc aagcagtcgc cggctgagct cgccaagctg
     1081 atgggattgt ccgccgacct ggcgga
//
LOCUS       DBNHQR010000556         4001 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1131498, whole genome shotgun sequence.
ACCESSION   DBNHQR010000556 DBNHQR010000000
VERSION     DBNHQR010000556.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4001)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1131498"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="AC16VR_12690"
     CDS             <1..532
                     /locus_tag="AC16VR_12690"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6012060.1"
                     /translation="VDSAPEIPPPLKVMLKNAGIDPASYDGPVGGIRKAVQQHMRESQ
                     AALGKDYSELNDDQLTDDYNYLIFPNVTCNTHADNLMLFRNRPHPEDPNRMLFDIWMF
                     ELLPEGEERPGLPRHDFRPEGSTRSLGLVIDQDASNLASVQAGMNSAAYPGLWLSDQE
                     LRLRHFHKTISDFVGD"
     gene            775..1935
                     /locus_tag="AC16VR_12695"
     CDS             775..1935
                     /locus_tag="AC16VR_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012061.1"
                     /translation="MKRIPKWLSGGGLAALLLVLGGQAFGKACADLIAGQHTLVGTVC
                     VGVADDSVQVEYTVIAGWSINSVHFFFDDKPTEGELSVNSCPLGPNFPMTDPKDPYDC
                     GNPKLGHFTYNTSGLNTSTYVFSLPLNGIDYLEHGDLCGSEFNIVAHADVTGPEGQTE
                     GAWAVSWGEMEIDGQMVSFPNYILVDPEDPHERPRGNWGFAGMFDMKVNCEGSFKCET
                     AFAKGDYTFETWPHLDFNSNRWGWLLGPFGKGFVANSESFPLYAGAGQNDISKGALAG
                     VVTVSWDTAYEANQVDFKHHNVAVSYNMNPEWFISSTAENDPTQLLPGSVHIFIGAEG
                     ELLTTAPGQYTYVLDQSEAEVYAYSMSPVTVDAEEFWVVAHAEVCSFVSGEE"
     gene            2099..3307
                     /locus_tag="AC16VR_12700"
     CDS             2099..3307
                     /locus_tag="AC16VR_12700"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6012062.1"
                     /translation="MKLEDVDPLDPDIVECPYEFYRALRERGVYRVPQRGFFLVGNYD
                     TAMQVIKDTDTFSSKSGVAVPRSPDGSHPSNPREVRTLLTADPPEHRKYRELVNKAFS
                     AKRVASWEPRIREIADELIEGFAGTGTCELVHDFAVPLPLIVICEAMGLPREMLRTFK
                     RWSDGIASLGGMITPEQRRQIAAEQADFAAYVTEMIEERRSNLGDDFVSDLIRARFDG
                     ARPLNIDELTSLVIQFLVAGNETTTNTIASGMWLLLTHPEQLQAAREDPGLIPNLVEE
                     VLRIESPVQAHFRAAARDTELDGVPIPAGAGVGVIFGCCNRDDDKFADAAEFDVRRDN
                     ASRHLAFSQGIHFCPGAPLARLESAIAFERLLERLGNLRLEGDPNDLVHVPSFTHRGL
                     KKLVVRFDPT"
     gene            3333..>4001
                     /locus_tag="AC16VR_12705"
     CDS             3333..>4001
                     /locus_tag="AC16VR_12705"
                     /inference="COORDINATES: protein motif:HMM:NF017197.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HIR6012063.1"
                     /translation="MSRKIWIPAVIALLLISIGLAAPLAIKRYVEGWLRDRGVAEARI
                     TDVDLNPFTGRVRVEYLDAPGAADESGSFEMLELDLELLGLVTGRVMVSRAILRDAVL
                     DVRRMPDGRFSVGGLMFPLAQAEQEVESDGEGPAGSPRPRLGLAGLLVENVTLTYDDG
                     MLSDRLYIRSLTIDRMVTWEPERAARLVAELELNGAPVGLEAEVFAFADERRATLSID
                     VDAFD"
BASE COUNT          729 a         1271 c         1259 g          742 t
ORIGIN      
        1 ggtggactcc gcgccggaga taccgccccc gctgaaggtc atgctgaaga acgccgggat
       61 cgatcccgct tcctacgacg gtcccgtggg cgggatccgc aaggccgtcc agcagcacat
      121 gcgggagagc caggcggccc ttggaaagga ctactcggag cttaacgacg atcagctgac
      181 cgacgattac aactacctga tcttccccaa cgtcacctgc aatacccacg ccgacaatct
      241 gatgctgttc cgcaaccggc cccacccgga ggatccgaac cggatgctat tcgacatctg
      301 gatgttcgag ctgctgcccg agggcgaaga gcggcctggg ctgcccaggc acgacttccg
      361 gccggaaggc agcacgcggt ctctcgggct cgtcatcgat caggacgcct cgaacctcgc
      421 gtccgtccag gccggcatga acagcgccgc gtatcccggg ctctggctct ccgaccagga
      481 gctccggctg cgccactttc acaagaccat cagcgacttc gtcggcgact gacgagcacc
      541 gcgcgacggc gggcctttca gcttggatac ggtcgtccga ccagttctgt gaactggtcg
      601 acacttttgc atttgcccta cgtaaccggg gcccacccgg acaaagcgga cacactcctt
      661 taccacaacg ccagcaccaa tcgtcagaat cttcagatcg gtaatccgac atagaaacag
      721 gagccggtcg cgccgagggg gatgcgcgtt ctctacaact aggaggtgca tctcgtgaag
      781 agaatcccga agtggctcag cggaggcggc ctcgccgctt tgcttctcgt gttaggtggt
      841 caggcctttg gcaaggcctg tgccgacctc attgcaggcc agcataccct cgtcggtacc
      901 gtttgcgtcg gcgttgccga tgacagcgtg caggtcgagt acacggtgat cgccggctgg
      961 tcgatcaact ctgttcactt cttcttcgac gacaagccga cggaaggaga gctttcggtg
     1021 aattcgtgtc cgctcggtcc gaattttccc atgaccgatc ccaaggatcc ttacgactgc
     1081 ggcaatccga agctcgggca cttcacctac aacacgagtg gcctgaacac ctcgacctac
     1141 gtcttcagcc tgccgttgaa cggcatcgac tatctcgagc atggtgatct ctgtgggtcc
     1201 gagttcaaca tcgttgccca cgccgatgtc acgggccccg aaggccaaac cgaaggcgct
     1261 tgggccgtca gctggggaga gatggagatc gatgggcaga tggtgagctt ccccaactac
     1321 atcctcgtgg atcctgaaga cccccatgag cgcccgcgcg ggaactgggg ctttgccggt
     1381 atgttcgaca tgaaggtcaa ctgcgaaggt agctttaagt gcgagacagc cttcgccaaa
     1441 ggggattaca cttttgagac ctggccccac cttgacttca acagcaatcg ttggggctgg
     1501 ctgctcgggc cgttcggcaa agggttcgtc gccaactcgg agtccttccc gctctacgcg
     1561 ggagccggtc agaacgacat atccaaaggc gctctggctg gtgtggtcac cgtgagctgg
     1621 gataccgcct acgaggccaa ccaggtggac ttcaagcatc acaacgtcgc ggtcagctac
     1681 aacatgaatc cggaatggtt catctcgagc accgcggaga acgacccgac ccagttgctc
     1741 cccggatccg tgcacatatt catcggtgct gagggggaac tcctcacgac ggcccctggc
     1801 cagtacacct acgtcctcga tcagagcgag gccgaggttt atgcttacag catgagcccg
     1861 gtgacggtgg atgccgagga attctgggtg gtggcgcacg ctgaggtttg ctcgttcgtc
     1921 tccggcgagg agtagatcga caggacactg ttcagaagaa caggcggatt ggagggtgct
     1981 tcggcaccct ctttttttgg ccgctcggtt acttgtgggg cgaggctgac gtccgggccg
     2041 attgcgcgcc ggcagcgctc cgcgcagaat cgcatcgggg ggtgggagtg aaccgcagat
     2101 gaagcttgaa gacgtcgatc cgctggatcc cgacatcgtc gagtgcccgt acgagtttta
     2161 ccgggcgctg cgggagcgcg gcgtctaccg ggtgccgcag cgcggattct tccttgtcgg
     2221 caactacgac accgccatgc aggtgatcaa ggatacggac accttttcgt cgaagtccgg
     2281 cgtggcggtg ccgagatccc cggacggcag ccacccgtcg aacccgcgcg aggtgcgcac
     2341 gctgctcacg gcggaccccc ccgagcaccg caagtaccgc gagctggtca acaaggcctt
     2401 ctccgccaag cgggtggcct cctgggaacc gcgcatccgg gagatcgccg acgagctgat
     2461 cgaaggcttt gccggcaccg gcacctgcga gctcgtccac gactttgccg tgcccctgcc
     2521 gctcatcgtc atctgcgagg ccatgggcct gccccgggaa atgcttcgca ccttcaagcg
     2581 ctggtcggac gggatcgcga gcctcggtgg catgatcacg ccggagcaac gccggcagat
     2641 cgccgcggag caggcggatt tcgccgcata cgtcaccgag atgatcgagg agcgcagatc
     2701 gaatctcggc gacgacttcg tctccgatct gatccgagcc cgcttcgacg gcgcacggcc
     2761 gctgaacatt gatgagctta cgagcctcgt catccagttt ctggtggcag gtaacgagac
     2821 caccaccaac accatcgcgt ccgggatgtg gctcctgctc acccatcctg agcagctcca
     2881 ggccgcgcgc gaggaccccg gcctgatccc caacctcgtc gaggaagtgc tgagaatcga
     2941 gtcgcccgtg caggcgcact tcagagctgc cgcccgggac acggagctcg acggcgtgcc
     3001 gatccccgcg ggagccgggg tgggcgtcat cttcggctgc tgcaatcggg acgatgacaa
     3061 gtttgccgac gcggcggaat tcgacgtccg gcgcgataac gcctcgcgtc acctcgcctt
     3121 cagccagggc attcacttct gcccgggcgc gccgctggcc cgtctggaaa gcgcgattgc
     3181 cttcgagcgc ctcctcgaac ggctcggcaa cctgcgtctc gaaggggatc cgaatgatct
     3241 tgtccacgtc cccagcttca cccatcgtgg cctgaagaag ctggtagttc gcttcgatcc
     3301 gacatgatga gatgcagacg ttgcccgtca gcgtgagccg aaagatctgg attccggccg
     3361 ttattgccct gctgctgatc agcatcgggc tggccgcgcc cctggccatc aagcgctacg
     3421 tggaaggctg gctgcgtgat cgcggcgttg ccgaggcccg tatcaccgac gtggacttga
     3481 accccttcac cggcagggta cgggtcgagt atctggatgc tcccggcgcc gccgatgagt
     3541 cgggcagctt cgagatgctg gagctggatc tggagctgct cggcctcgtt accgggcggg
     3601 tgatggtttc gcgggccatc ctgcgggatg ccgtcctgga cgtgcggcgg atgccggacg
     3661 ggcgcttctc cgtgggtggc ctgatgttcc ccctggccca ggcggaacag gaagtggaga
     3721 gcgacggcga gggtcccgcc ggatcaccgc gtcccaggct cggcttagcc ggactcctgg
     3781 tggagaacgt gacccttacc tacgacgacg ggatgctcag cgatcggctc tacatccgtt
     3841 ctctgaccat cgatcgcatg gtcacctggg aacccgagag ggccgctcgg ctggttgccg
     3901 agctcgagct gaacggggcg ccggttgggc tggaggcgga ggtgttcgcg ttcgcagacg
     3961 agcggcgcgc caccctatcg atcgacgtgg atgctttcga t
//
LOCUS       DBNHQR010000557         1520 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1383100, whole genome shotgun sequence.
ACCESSION   DBNHQR010000557 DBNHQR010000000
VERSION     DBNHQR010000557.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1383100"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="AC16VR_12710"
     CDS             <1..219
                     /locus_tag="AC16VR_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012064.1"
                     /translation="VFHIVEVPLERTSQLGELPELVTTEEDMRERVIEAHRTLMSMNE
                     SNRETFQDLVAALEAEEQPVMERRRASR"
     gene            complement(191..643)
                     /locus_tag="AC16VR_12715"
     CDS             complement(191..643)
                     /locus_tag="AC16VR_12715"
                     /inference="COORDINATES: protein motif:HMM:NF016955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HIR6012065.1"
                     /translation="MLSQLLARLKEPLANSEAETAALLPLAAAVLYLEVAWADHDIAD
                     EEMQLIRRTLSEQFDLTAEEVDVIVSESKEQHDASVGLYRFTRTITEAWDEETRFQLV
                     VRLWGLALSDDELHSFEEHTIRKIAELLYVSHSRFIEAKLIAKRDAAP"
     gene            complement(663..>1520)
                     /gene="dusA"
                     /locus_tag="AC16VR_12720"
     CDS             complement(663..>1520)
                     /gene="dusA"
                     /locus_tag="AC16VR_12720"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: protein motif:HMM:NF008774.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HIR6012066.1"
                     /translation="TTGALLNGPRRELLAFDPEEHPVAIQLGGCEPADLARAAAIAQT
                     AGFDEINLNLGCPSPRVSKGRFGACLMREPELAAACIGAMRDACRLPVTAKIRLGVDD
                     HDSDALLHDFVARLVEAGCHALYLHARKAYLSGLSPAQNRDIPPLQPERAYGLKRQFA
                     ELPIILNGGISREEEAAEHLRHVDGVMIGRAAYHDPMFLSRLDARLSGRAPVPLEAVV
                     TGYLAYMDRQLLRGTRLHDMTRHMLGLFKGVPGARRYRRLLSDSARLKANDLNLVHEA
                     IAATTAQAA"
BASE COUNT          260 a          475 c          507 g          278 t
ORIGIN      
        1 gtcttccata tcgtggaggt ccctctggag cgcacgtccc agttgggcga gctccccgag
       61 ttggtgacca ctgaagaaga catgcgtgag cgagtcatcg aagctcaccg gacactgatg
      121 tcgatgaacg aatccaatcg cgagacgttt caggaccttg tcgccgctct cgaagccgaa
      181 gagcagcccg ttatggagcg gcgtcgcgct tcgcgatgag cttggcctcg ataaaacggc
      241 tatggctgac atacagcagc tcggcgatct tgcggatagt gtgctcttcg aagctgtgca
      301 gctcgtcgtc tgacagtgcc agaccccaga gccggacgac gagctgaaac ctggtttcct
      361 cgtcccacgc ctcggtgatg gtgcgggtga atcggtacag ccccacgctg gcgtcgtgct
      421 gctctttgga ttcgctcacg atcacgtcca cctcttcggc cgtgaggtcg aactgctccg
      481 agagcgttcg gcggatcagc tgcatttcct catcggcgat gtcgtgatcg gcccaggcga
      541 cttccaggta caaaacggct gcggccaggg gtagcagcgc cgccgtttcg gcttccgaat
      601 tcgccagcgg ttccttgagc cgagcgagca gctggctgag catcaggcgg tctccatggg
      661 agtcatgctg cctgtgccgt ggtcgccgcg atcgcttcgt ggacgaggtt cagatcgttc
      721 gccttcagcc gcgccgagtc gctcagcagc cggcggtaac gtctggcgcc ggggacgcct
      781 ttgaacaggc ccagcatgtg gcgtgtcatg tcgtgcagac gcgtgcctcg aaggagttgt
      841 cggtccatgt aggccaggta gccggtgacc acggcttcca gaggcaccgg agcgcggcct
      901 gacagcctgg catccagccg cgacagaaac atggggtcgt gataagcggc ccgaccgatc
      961 atgactccgt cgacgtgtcg aagatgctcc gcggcctctt cctcgcggga tatgcccccg
     1021 ttgagaatga tcggcagctc ggcgaactgc ctcttgaggc cgtacgcgcg ctccggctgc
     1081 agcgggggaa tgtcccggtt ctgcgcgggc gaaaggccac tcaagtaagc cttgcgggcg
     1141 tgcaggtaca acgcgtggca accggcctcg acgagccgcg caacgaaatc atggagcagg
     1201 gcgtcggagt cgtggtcgtc tacccccagc cggatcttcg cggtgacggg cagcctgcag
     1261 gcgtcgcgca tggccccgat gcaggcggcg gcgagctcgg gttcccgcat caggcacgcg
     1321 ccgaagcggc ccttgctcac cctgggtgaa gggcagccga gatttaggtt gatctcgtcg
     1381 aacccggcgg tctgggcaat cgcggcagcc cgggcaagat ccgccggctc gcagccgccg
     1441 agctgaatgg ccaccggatg ctcctcaggg tcaaaggcga gcagctctcg ccggggcccg
     1501 ttcagcagcg cgccggtggt
//
LOCUS       DBNHQR010000558         3729 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1447860, whole genome shotgun sequence.
ACCESSION   DBNHQR010000558 DBNHQR010000000
VERSION     DBNHQR010000558.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1447860"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="AC16VR_12725"
     CDS             <1..533
                     /locus_tag="AC16VR_12725"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF019555.7"
                     /note="penicillin-binding protein 5; removes C-terminal
                     D-alanyl residues from sugar-peptide cell wall precursors;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HIR6012067.1"
                     /translation="NRLLWRDRTVDGVKTGHTSAAGYCLVASAKRGNMRLISVVLGTE
                     SEEARMRESQKLLSYGFRYFETQKLYDAGVPLKTSKIWYGDADDIQLGLAEEVFVTIP
                     RGHYEDLKAELSVVKTIEAPVAAGQELGELRLHFGDELMLSAPLVALQEVKEADMFSR
                     LLDGTRLLFRDLFGVD"
     gene            526..789
                     /locus_tag="AC16VR_12730"
     CDS             526..789
                     /locus_tag="AC16VR_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014420668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HP0495 family protein"
                     /protein_id="HIR6012068.1"
                     /translation="MTEPPKIEFPCDYPIRVIAENHLALRETVVRIVRLHAPDLAEST
                     VSVRNSREGRYCSVRFSITARGEPQLRALHRALMDEPMVKMVL"
     gene            809..1438
                     /gene="lipB"
                     /locus_tag="AC16VR_12735"
     CDS             809..1438
                     /gene="lipB"
                     /locus_tag="AC16VR_12735"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020295282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HIR6012069.1"
                     /translation="MVRQLGRTRYEPVFRAMQVFTEERAEPTADELWFTEHDPVFTQG
                     QAGKAEHLLAPGDIPVVQTDRGGQVTYHGPGQIVAYLLFDLRRMGLSVRGLVSGIEEA
                     VVRLLADYDIRSAPRRDAPGVYVAGSKIAALGLRVRRGYSYHGLALNVQMDLEPFSRI
                     NPCGLFGMEVTQLADLGGPADVQTVEDELLTKLADVYGFTPSSPDTQGP"
     gene            complement(1404..2108)
                     /locus_tag="AC16VR_12740"
     CDS             complement(1404..2108)
                     /locus_tag="AC16VR_12740"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017641082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HIR6012070.1"
                     /translation="MLPEVDVAGQESLLAGTVLIVGMGGLGAPAAIYLASAGVGHLII
                     SDHDRVEASNLQRQIVHHDASIGQTKVASACQSLLQTNPELRITGIDRKLEGDELLKI
                     VGDADVVLDATDNFAARFAINAACWEAGVPLVSGAAIRWEGQLAVFDPRDAASPCYRC
                     LYAEGHDEALNCAENGVIAPLVGVIGTCQAIEAIKLLTGIGEPLTGYVLYFDAKRMDW
                     RKLKLPKDPACPGCSA"
     gene            complement(2153..2953)
                     /gene="prmC"
                     /locus_tag="AC16VR_12745"
     CDS             complement(2153..2953)
                     /gene="prmC"
                     /locus_tag="AC16VR_12745"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIR6012071.1"
                     /translation="MTTVRAWIDRERELDLLDRHLLLAELLQTNRAGVMTYPERVLND
                     AELRTLDLWSSRRRHGEPMAYILGRKDFWGLTLRVNPTVLIPRPETELLVESALEVAA
                     AGQRLLDLGTGSGAVAIALARELANAAVSASDVSQAALKLAAANAAAHGARLTFIASH
                     WFENVTPSYDVIVSNPPYVAERDAHLTSLRAEPTAALVSGADGLDAIRYIVPAARRYL
                     NEAGWLIVEHGHDQGSSVRSLMRCSGYREVTTRRDLAGLERVTRGRRP"
     gene            complement(2950..>3729)
                     /gene="prfA"
                     /locus_tag="AC16VR_12750"
     CDS             complement(2950..>3729)
                     /gene="prfA"
                     /locus_tag="AC16VR_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014420703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIR6012072.1"
                     /translation="PRDPSDASNVYLEIRAGTGGDEAALFAGDLFRMYGKFAESRGWS
                     FEVLGARAGEHGGYKEIITRVAGKDVYSQLKFESGAHRVQRVPETESQGRIHTSACTV
                     AVMPEVDEVDSIEINKSDLREDTFRASGAGGQHVNKTDSAIRLTHLPSGIVVECQDER
                     SQHKNRARALALLQAKLLDAAQSQQRSEQAETRRNLVGSGDRSERIRTYNFPQGRVTD
                     HRINLTLYKLTEVMEGALENVIDPLVREHHADQLRSLGDEQ"
BASE COUNT          597 a         1202 c         1258 g          672 t
ORIGIN      
        1 gcaaccggct gctctggcgg gaccgcaccg tggacggggt caagaccggg cacacctcag
       61 ccgccggcta ctgcctggtc gcctcggcca agcgcggcaa catgcgattg atttccgtcg
      121 tgctgggcac ggaaagcgaa gaggccagga tgcgcgagag ccagaagctg ctttcctacg
      181 gatttcgcta tttcgagacg cagaaactct acgatgccgg tgtccctttg aagacctcga
      241 agatctggta cggcgatgcg gacgacatcc agctgggcct tgcagaggag gttttcgtaa
      301 ccatcccccg gggccactac gaagacctca aggccgagct cagcgtggtg aagaccatcg
      361 aggctcccgt agctgccggt caggagctgg gggaactgcg tttgcacttc ggtgacgagc
      421 tgatgttgag cgcgcccctg gtcgcgttgc aggaagtcaa ggaagcagac atgttctcgc
      481 gcctgctgga cggcacccgg ctcctctttc gcgacctttt cggcgttgac tgaacccccg
      541 aaaatcgagt ttccctgcga ctaccccatc cgggtgatcg cggagaatca cctcgcgctc
      601 cgcgagaccg tggtgcggat cgttcgcctg catgccccgg acctggcgga gagcaccgta
      661 tcggtcagga acagccgcga aggccgctac tgctcggtgc gcttctcgat aaccgccagg
      721 ggggaacccc agctgcgggc cctgcatcgg gctctcatgg acgaacccat ggtcaagatg
      781 gtgctgtgag gtggctgatg tccccgtaat cgtcaggcag ttggggcgga cccgttacga
      841 gccggtattt cgagccatgc aggtcttcac cgaggaacgg gcggagccca ccgccgatga
      901 gctctggttc accgagcacg atccggtgtt cacccagggt caggcgggca aggccgagca
      961 cctcctggcg ccgggcgaca ttccggtggt gcaaactgac cggggcgggc aggtgaccta
     1021 tcacggcccg ggccagatcg tcgcctatct gctgttcgac ctgcgtcgca tggggctgag
     1081 cgtgcgcggc ctggtcagcg gcatcgagga agccgtcgtc aggctgctgg ccgactacga
     1141 tatccgcagc gcgccgcgcc gtgatgcgcc gggggtctac gtggccgggt ctaagatcgc
     1201 ggctctcggg ctgagagttc ggcgcgggta ctcctaccac ggactggctc tcaatgtgca
     1261 gatggactta gagccgttct cgcgcatcaa cccgtgcggg ttgttcggca tggaggtgac
     1321 gcagcttgcc gacctgggcg gtccggcgga cgtgcagacc gtcgaggatg agctcctgac
     1381 gaagctggcc gacgtctacg ggttcacgcc gagcagcccg gacacgcagg gtccttaggc
     1441 agcttcagct ttcgccaatc catgcgtttg gcgtcgaagt agaggacgta gccggtcaac
     1501 ggctcgccga ttccggtgag cagcttgatg gcttctatgg cctggcaggt gccgatgact
     1561 cccaccagag gggcgatgac cccgttctcg gcgcagttca gcgcctcgtc gtgtccctcg
     1621 gcgtaaaggc agcggtaaca ggggctcgct gcatcccgcg ggtcgaagac ggcgagctga
     1681 ccttcccagc ggatggcggc gcctgagacc agggggactc cggcctccca gcaggcggcg
     1741 ttgatcgcga agcgggcggc aaagttgtcg gtggcgtcca gcaccacgtc ggcgtcaccc
     1801 acgatcttta gcagctcgtc cccctccagc tttcggtcga tgccggtgat ccgcagttcc
     1861 gggttggtct gcagcagcga ctggcatgcc gatgccacct tggtctggcc gatgctggcg
     1921 tcgtggtgga cgatctgtcg ctgcaggttg gaagcctcca cccgatcgtg atcgctgatg
     1981 atgaggtggc ccacgcctgc gcttgccagg taaatggcgg cgggcgcgcc gagcccaccc
     2041 atgccgacga tgagcacggt cccggcgagc agggattcct ggccggcgac gtcgacctcc
     2101 ggcagcatga tctggcggct gtatcgaagc agctgatcgt cgctcagccc attcaaggtc
     2161 ttcggcctcg cgtgacgcgc tctagcccgg cgaggtcccg tcgcgtcgtc acctcgcggt
     2221 agccgctgca acgcatgagc gaacggaccg agctgccctg atcgtggccg tgctcgacga
     2281 tgagccaacc ggcttcgttg aggtagcgcc tggcggcggg aacgatgtag cgaatggcgt
     2341 ccagcccgtc tgcgccgctc accagcgcgg cggtgggctc ggcccgaagc gaggtgagat
     2401 gggcatcgcg ctcggcaacg taaggcgggt tgctgacgat gacatcgtag gaaggggtga
     2461 cgttctcgaa ccagtggctc gcaatgaacg tcagcctggc gccgtgggct gcggcatttg
     2521 cagccgcgag cttgagcgct gcctgggaga cgtcgcttgc gctcaccgcc gcgttcgcga
     2581 gctcccgggc cagcgcgatg gcgacggcgc cgctgcccgt gccgagatcc agtaggcgct
     2641 gaccggccgc ggctacctcc agcgcgctct ccaccagcag ctccgtctcg ggtcggggga
     2701 tcaataccgt cgggttcacc cgaagcgtca gcccccagaa atccttgcgg ccgaggatgt
     2761 aggccatggg ctcgccgtgg cgccgacggc tcgaccagag gtccagggtg cgaagttccg
     2821 cgtcgttcag aacgcgttcc ggataggtca tgacgccggc gcggttggtt tgcagcagct
     2881 cggcgagcag caggtggcga tccagcagat ccagctcccg ctccctgtcg atccaggcac
     2941 ggaccgtggt cactgctcgt cgcccaggct gcgcagctga tccgcgtgat gctcacgcac
     3001 cagggggtcg atcacgtttt ccagtgcccc ctccatgacc tcggtcagtt tgtagagggt
     3061 caggttgatc cgatgatcgg tgacgcgacc ctggggaaag ttgtaggtgc ggatgcgctc
     3121 cgagcggtcg cccgagccca ccagattacg gcgcgtctcc gcctgctcgg atcgttgctg
     3181 cgactgggcg gcgtcgagca gcttcgcctg gagcagcgcc agcgcccgcg cgcggttctt
     3241 gtgctgcgag cgctcgtcct gacactccac gacgatgccg ctcggcaggt gagtcagccg
     3301 gatggccgag tccgtcttgt tcacgtgctg tccgccggcc ccggaggcgc ggaaggtgtc
     3361 ctcgcgcaga tcggacttgt tgatctcgat ggagtccacc tcgtccacct ccggcatcac
     3421 ggccacggtg cacgcggacg tgtgtatgcg tccctgggac tccgtttcgg gaacgcgctg
     3481 cacccggtgc gcgcctgact cgaatttcag ctggctgtag acgtccttgc cggcgacacg
     3541 ggtgatgatc tccttgtatc cgccatgctc gcctgcccgg gcgcccagca cttcgaagct
     3601 ccagccgcgg gactccgcga acttgccgta catgcggaag aggtccccgg caaacagcgc
     3661 cgcctcatca ccacccgtac ccgcgcgaat ctccaggtag acgttcgacg cgtcgctggg
     3721 gtcccgtgg
//
LOCUS       DBNHQR010000559         6540 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_878325, whole genome shotgun sequence.
ACCESSION   DBNHQR010000559 DBNHQR010000000
VERSION     DBNHQR010000559.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6540)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6540)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6540
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_878325"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..114)
                     /locus_tag="AC16VR_12755"
     CDS             complement(<1..114)
                     /locus_tag="AC16VR_12755"
                     /inference="COORDINATES: protein motif:HMM:NF019814.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIR6012073.1"
                     /translation="MATVSTNEFRSGLKVLLEGEPCSILENEFVKPGKGQAF"
     gene            359..2476
                     /locus_tag="AC16VR_12760"
     CDS             359..2476
                     /locus_tag="AC16VR_12760"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR6012074.1"
                     /translation="MAKKDMTAQHNPSAPVRLLIAENSENAAQEFDSLLRDAGIPTRS
                     EIIDLPMAVDAMHEADLLLANSSLPELEQIIPRLSDQAPNVPIVLVNYDDATITTTRG
                     MQLGAADVVPKSEPEHLVLVVKRELEHVLQYQSLSQTRRALQETEQRCQLLLRSSKAA
                     IAYVHDGMHIYANEGYLKLFGFEDVEDLIGLPHMDLLDADSAVALKVAMKQFQRDGEE
                     KEIEFVGQNTAEETIAGTMTLAAAEYEGEHCMQVTVRTGRATDAPATAQAAADDASTA
                     NGEGSAPADVRLHAFFDAVAGNPRTRSEDAFSAIFVAEIDDYAEVQRSHGLAGALEVC
                     RHVERCLGELIASDALHRLADHQFAFAVTADNEDEVRAEADQLRGTIEASLLEVREKT
                     VRPTITLAGMEIATEEDQGVRERLEDCLNEAYDVLRTSMEQNEGNCVELKSQGAGDEP
                     ESESARLLRLINEAIDDQRFVLLFQPMISLHGDSDEHYEVLLRMLDDEGKQMLPGAFL
                     RTAIENNLAGRIDRWVILQAIKSLSAHRAKGNLTRLTINLTSNSVNDPEFLEWLGVAF
                     KAVRLPTDAVVLQITEQDANTYVRQTREFFEQLKQMHCVASLSRFGRLGNSFELLEQI
                     PAEYVKVDGSLIEKIASSSEAREALTEMIHGLQGAGKLTVVPMVESANILSTLWQAGA
                     NYVAGHYLQEPSRSMDYDFSADD"
     gene            complement(2511..3749)
                     /gene="serB"
                     /locus_tag="AC16VR_12765"
     CDS             complement(2511..3749)
                     /gene="serB"
                     /locus_tag="AC16VR_12765"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HIR6012075.1"
                     /translation="MSSEIVLVNVSGEDQPGLMAMLTSTLARYQVRVLDVGQAVIHDE
                     LNLGMLLQIISDGNGVEAAEVLSAIEANVLDQGMTARFTPISAGDYADWVKSQGKPRY
                     IVTLLTAGATGEQLAAVTSIVRHHGLNIDAIRRLSGRLPLTSDAGGQRICIEMSLRGV
                     LADAGALKAGLLEAAERLAFDFSVQQDTVYRRNRRLVAFDMDSTLIDAEVIDELAAAH
                     GVGEQVARITERAMRGELKFADSFRERAMLLKGLSEDALREVADGVALNDGARRLIRT
                     LKHFGYKTAIISGGFQYVGERLRDELGIDYVYANSLEIRDGLMSGEVEGEIIDAGRKA
                     ELLKEIARREGISGQQTIAIGDGANDLPMLNAAGLGVAYHAKPMVKETASHAISTFGL
                     DSVLYLMGFSDRDIDEALTG"
     gene            3775..4896
                     /locus_tag="AC16VR_12770"
     CDS             3775..4896
                     /locus_tag="AC16VR_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012076.1"
                     /translation="MKLTSWLGLVKPPPLSLVGACLGSLVSVAVMLSYWALNDPDQLR
                     HDAYGRSMSTGLAQLAIEPLLTHDRITLGVIANQAADIPGVSGVEIVTVNEQALASSG
                     SLSNARVYQSPVTFNDQIMGAVRVALSEPPSHLGDAVAVAAVTLIAAPFLALALLSIR
                     LQPPLPRQPARIEVELHTPRERHFLVAVNLYDQLGMEPTQRVAELNYARIAAGKVAGL
                     YGGDVENLPGTGLLLDFNDGGDADRPWQVLSAAAVLSELLDEEAGDYRVAIHVGDAPV
                     GHRVLLDQQAVGDAALLSALAKPATLAASSVFSACLDRGGELVERTVIEHPLLEELAT
                     VGPDSVLITALSAPQQDLVQHQAAHLAGHRPATASESTF"
     gene            complement(4860..>6540)
                     /gene="parC"
                     /locus_tag="AC16VR_12775"
     CDS             complement(4860..>6540)
                     /gene="parC"
                     /locus_tag="AC16VR_12775"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HIR6012077.1"
                     /translation="NEVISACIHLLDSPKATVADLMTHIKAPDFPSEAIIITPPAEIR
                     EAYETGRGSVKARAVWKKEAGDIVVTALPHQASPAKVLEQIAQQMNAKKLPMLADLRD
                     ESDHENPTRLVLVPRSNRVDAERLMSHLFATTDLEKSFRINLNIIGLDQRPQVKSLQA
                     ILKEWLTYRLETVRRRLTFRLDRINERLHILDGLLKAYLNLDEVIRIIREDDHPKASL
                     MKRFTLSEIQANAILDLRLRQLAKLEEDKLKAEKSELESEKADIEKTLKSKARMKALL
                     KSELVEIAEGYGDARRSPLGAVEDAAAYSEEDLISNEPITVVLSEKGWVRSAKGHETN
                     PEELSYRSGDGYGHAARGRTNDILVFVDSTGRCYTVAAHSLPSARGQGEPLTGRLKPP
                     AGSRFVGVALGPGESKVLLASDAGYGFVGRLSGLVAKNKSGKACLSVPKGGLALPPLL
                     LADQPDLQVVAVTDQGRMLVFPLTEVPELTRGKGNKLLNIPSAAFKSGEERLVAVTVI
                     RPDDELLVLAGQRHLRMKHRDLENYVGERARRGRKLPRGFQKVDSLAVAGR"
BASE COUNT         1167 a         2151 c         2052 g         1170 t
ORIGIN      
        1 gaaggcctgg cccttccccg gcttcacgaa ctcgttctcg aggatcgagc agggctcgcc
       61 ctccagaagg accttcagtc ccgagcggaa ctcgttggta gaaacagtcg ccatgtgcag
      121 cggaccagtg tggaatcggc ggcatcatat ccgcttggcc gtcgagggga tagccggctt
      181 cggagcggcg ccggggcttc cggacttgct cggattcacg ctctctggtc aaagcgcgac
      241 ccggttcaca tttcgtctgt tggatggtta cttggattgg ccacgtttcg gccgcaccat
      301 gagttcctcc gcggacgtcc aatgcccgac gtccgggtga ttcgaaaaaa gggaaaggtt
      361 ggctaaaaag gatatgacgg ctcagcacaa tcccagcgcg cccgtgcgcc tgctaattgc
      421 ggagaattcc gagaatgcgg cgcaggagtt cgactcgctc ctgcgcgacg caggcattcc
      481 cacccggtcg gagatcatcg atcttcccat ggccgtcgac gccatgcacg aagcggatct
      541 gctgctggcg aactcttctc tgcccgagct agagcagatc atcccgcggc tcagcgacca
      601 ggcgcccaac gttccgatcg ttctcgtcaa ttacgatgac gcgacgatca cgaccacgcg
      661 cggcatgcag ctcggcgccg ccgacgtggt cccgaaatcc gagccggagc acctcgtcct
      721 ggtggtcaag cgggagctcg agcacgttct ccagtaccag agcctgagcc agacacgccg
      781 ggcactccag gaaaccgagc agcgctgtca gctgctgctc agaagctcca aagccgccat
      841 cgcctacgtc cacgacggca tgcacatcta cgccaacgag ggttacctga agctcttcgg
      901 ctttgaagac gtggaggacc tcatcgggct gccgcacatg gatctgctcg acgccgacag
      961 cgccgtggcc ttgaaggtcg cgatgaagca gttccagcgc gacggcgagg aaaaagagat
     1021 cgagttcgtc ggtcagaaca cggccgaaga gaccattgcg ggcaccatga ccctcgccgc
     1081 cgccgagtac gagggcgagc actgcatgca ggtcaccgtg cgaaccggcc gggcaaccga
     1141 cgcccctgcg accgcgcagg ccgcagccga cgatgcttcg actgccaacg gcgaaggcag
     1201 cgcgccagcc gatgtgaggc ttcacgcctt cttcgacgcg gtggccggca accccaggac
     1261 ccgctccgag gacgcatttt ccgcgatttt cgtcgccgag atcgacgatt acgccgaggt
     1321 gcagcgatct cacggtctgg ctggagcgct cgaggtctgc cgtcatgtcg agcggtgtct
     1381 cggcgagctg atcgcatcgg acgcgctgca tcgactggcg gatcatcagt tcgcctttgc
     1441 ggtaaccgcc gacaacgagg acgaggtccg agcagaggcc gatcagcttc gcgggaccat
     1501 cgaggcatcg ctgctcgagg tcagggagaa gaccgtacgg cccacgatta ccctcgccgg
     1561 catggagatc gccacggagg aagatcaggg cgtccgtgag cggttggagg actgtctgaa
     1621 cgaggcctac gatgtcctgc gcacatccat ggaacagaac gaaggaaact gcgtcgagct
     1681 gaagtcccag ggagcaggcg acgagccgga gtccgaatca gcaaggctgc tgcgcctgat
     1741 caacgaggcc atcgacgacc agcgattcgt tctgctgttc cagccgatga tcagcctgca
     1801 cggcgactcc gacgagcact acgaagtgct gctgcgcatg cttgacgacg aaggcaaaca
     1861 gatgctcccg ggcgctttcc tgcgcacggc catcgagaac aacctcgccg gacggatcga
     1921 cagatgggtc attctccagg cgatcaagtc gctgtctgcg caccgtgcca agggcaacct
     1981 gacccggctg accatcaacc tgaccagcaa cagcgtcaac gacccggagt tcctggagtg
     2041 gcttggcgtc gccttcaagg ccgttcggct gcctaccgat gcggtggtcc tgcagatcac
     2101 ggagcaggac gccaacacct acgttcgcca gacccgcgag ttcttcgagc agttgaagca
     2161 gatgcactgc gtagcctcgc tgtcgcgatt tgggcgcctc ggcaactctt tcgaactgct
     2221 cgaacaaatc ccggcggaat acgtcaaggt cgacggttcg ctgatcgaga agatcgccag
     2281 cagctcggag gcgcgtgagg cgctgaccga gatgattcac ggcctccagg gcgccggcaa
     2341 gctcacggtg gtccccatgg tggaaagcgc caacatcctg tcgaccctat ggcaggccgg
     2401 tgccaactac gtggccgggc actacctgca ggagccctcc agaagtatgg attacgactt
     2461 cagcgccgac gactgatccc cgctgaccgg atatcgccgg ggcggcggct tcagcccgta
     2521 agcgcttcgt cgatgtcgcg gtcgctgaag cccatcaggt acagaacgct gtccaggccg
     2581 aaggtggaga tggcgtggct agccgtctcc ttgaccatgg gtttcgcgtg gtatgccacc
     2641 ccaagaccgg cggcattgag catgggcagg tcattcgcgc cgtcgccgat ggcgatggtc
     2701 tgctggccgg atatcccctc acggcgggcg atctccttca agagctcggc cttgcggccg
     2761 gcgtctatga tctccccttc gacctcgccc gacatcagcc cgtcgcgaat ctccagcgag
     2821 ttggcataaa cgtaatcgat gcccagctcg tcacgcagcc gctcaccaac gtactgaaag
     2881 ccgcctgaga tgatggccgt cttgtagccg aaatgcttga gcgtgcggat caggcgccgg
     2941 gcaccgtcgt tcagcgcaac gccgtctgcc acctcacgca gggcgtcctc ggaaagaccc
     3001 ttgagcagca tggcccgctc tcgaaagctg tcggcgaact tcagctcacc gcgcatggct
     3061 cgctcggtga tccgagctac ctgctcgcca accccgtgcg cggccgccag ctcgtcgatg
     3121 acctcggcat cgatgagcgt agagtccatg tcgaacgcca ccagccgccg gttgcgccgg
     3181 tacacggtgt cctgctgcac gctgaaatcg aacgccaggc gctcggcggc ttcgagaagg
     3241 cccgctttca gggcgccggc atcggcaagg acgccgcgca ggctcatctc gatgcagatc
     3301 cgctgaccac cggcgtcgga cgtcaagggc aagcgccccg acagccgtcg tatggcgtcg
     3361 atgttgaggc cgtggtgacg aacgatggag gtcaccgcgg cgagctgctc gccggtggcg
     3421 ccggccgtga ggagggtgac gatatagcgg ggcttgccct ggctctttac ccagtcggcg
     3481 tagtcgcccg ccgagatggg agtgaagcgc gccgtcatgc cctgatcgag aacgttcgcc
     3541 tcgatggcgg acagcacctc ggcggcctcc acgccgttac cgtcactgat gatctgcagc
     3601 aacatgccga ggttgagctc gtcgtggatc accgcctgcc ccacgtcgag cacccgcact
     3661 tgataacgtg ccagagtgga tgtcaacatg gccatcaggc caggctggtc ctctccggac
     3721 acgttcacca atacaatctc gctgctcaca tcgccgcgcg gaataggaag tagtttgaag
     3781 ctaacgtctt ggctggggct cgtcaaacca ccgccgcttt cgctggtcgg cgcttgcctg
     3841 ggctcgctgg tttccgtggc cgtgatgctg tcctactggg ctctcaacga tcccgatcag
     3901 ctccgtcacg acgcctacgg caggagcatg tccacagggc tcgcccagct ggccatcgag
     3961 ccgctgctga cccacgatcg catcacgctg ggcgtgatcg ccaatcaggc ggcggacatt
     4021 cccggcgtca gcggggtcga aatcgtaacg gtcaacgagc aggcgctcgc ctcgagcggc
     4081 tccctttcga acgcgcgcgt ctaccagagc ccggtgacct tcaacgacca gatcatgggc
     4141 gccgtgcggg tcgccctgag cgagcctcca agccacctcg gggatgccgt ggcggtggcc
     4201 gctgtcacct tgattgctgc gcccttcctg gcgctggccc tgctcagcat ccggcttcaa
     4261 ccgcccctgc cgaggcagcc cgcgcgtata gaggtggagc ttcacacgcc gcgggaaagg
     4321 cacttcctcg tcgccgtgaa cctgtacgac cagctcggca tggagcccac gcaacgcgtc
     4381 gccgagctga actacgcgcg catcgccgcg ggcaaggtcg ccggcctgta cggcggcgat
     4441 gtggaaaatc tgcccggtac cggattgctg ctggacttca acgacggggg cgatgcggac
     4501 cgtccctggc aggtgctgag cgcagcggcg gtgctctcgg agcttctcga cgaggaggcg
     4561 ggtgactatc gagtggccat tcacgtcggc gacgcgccgg tcggccatcg cgtgctgttg
     4621 gaccagcagg cggtcggcga tgccgcgctc ctgtccgcgc tcgccaagcc ggccaccctg
     4681 gccgcgagca gcgttttctc cgcctgcctc gaccgcggcg gcgagctggt ggagcgcacg
     4741 gtcatcgagc accccctgct cgaggagctg gcaacggtgg gtcccgactc ggtcctgatt
     4801 acagcgctgt cagccccgca gcaggatctg gtacagcacc aggccgccca cctcgccggt
     4861 caccgtcccg caactgccag cgagtctact ttctgaaatc cgcgcggaag cttgcgcccg
     4921 cgccgtgccc tctcaccaac gtagttctcg agatccctgt gcttcatgcg gagatgacgc
     4981 tgccccgcca gcacgagcag ctcgtcgtcg ggacgaatga ccgtcaccgc aaccagccgc
     5041 tcttcgccgg atttgaaagc cgcggacgga atgttgagaa gcttgtttcc cttgccgcgg
     5101 gtcagttcgg gcacctccgt cagcgggaag acgagcatcc gaccctggtc ggtgacggcc
     5161 accacctgca ggtccggctg atcggcgagc agcagggggg gcagggcgag gccccccttg
     5221 ggcacgctga gacaagcttt acctgacttg ttcttggcga cgagccccga cagccggccg
     5281 acgaaaccgt agccagcgtc gcttgccagc agcaccttcg actcgcccgg tccgagggcg
     5341 acgcccacga acctcgatcc cgcgggaggt ttgagccgcc cggtgagcgg ctcgccctgt
     5401 ccccgggcag aggggaggct gtgagccgcc acggtatagc agcggcccgt gctgtccaca
     5461 aagaccagaa tgtcgttggt tcggccccgg gccgcgtgcc cgtagccgtc gccgctccgg
     5521 tagctcagct cctcggggtt tgtttcgtgg cctttggcgg accggaccca tcccttctcc
     5581 gagagcacca ccgtgatcgg ctcgttgctg atcagatctt cctcgctgta ggccgccgca
     5641 tcttccaccg cacccagagg cgatcggcga gcgtcgccgt aaccctcggc gatctcgacc
     5701 agttcggact tcaacagggc cttcatgcgg gctttcgatt tgagcgtctt ctcgatgtcc
     5761 gctttttccg actcgagttc cgacttctcg gccttgagct tgtcctcctc cagcttggca
     5821 agttgccgca ggcgaaggtc caggatggcg ttggcctgga tttccgacag cgtgaaccgc
     5881 ttcatcaggg aagccttcgg gtgatcgtct tcccggatga tccggatcac ctcgtcgagg
     5941 ttcagatagg ccttgagcag gccgtcgagg atgtgcagtc gctcgttgat ccggtcgaga
     6001 cgaaacgtga gccgccggcg cacggtttcc aggcgatagg tcaaccactc cttgagaatg
     6061 gcctgcaagg atttcacctg agggcgctgg tccagaccga tgatattcag gttgatccgg
     6121 aaacttttct ccagatccgt ggtggcaaac aggtggctca tcagacgctc ggcatccacc
     6181 cgattggaac gaggcaccag gaccagccgg gtcgggttct cgtggtccga ctcgtcacgc
     6241 agatccgcca gcatcggcag cttcttcgcg ttcatctgct gggcgatctg ctctagcacc
     6301 ttagccggcg acgcctgatg gggcagcgcc gtgacgacga tgtccccggc ctcctttttc
     6361 cacaccgcgc gcgccttcac cgatccgcgc ccggtctcgt aggcctcgcg gatctctgcc
     6421 ggcggggtga tgatgatggc ttcggacggg aagtccggag ccttgatgtg cgtcatgagg
     6481 tcggcaacgg tggccttcgg cgagtcgagc aggtgaatgc aggcgctgat gacctcgttc
//
LOCUS       DBNHQR010000560         6267 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_182722, whole genome shotgun sequence.
ACCESSION   DBNHQR010000560 DBNHQR010000000
VERSION     DBNHQR010000560.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6267)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6267)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6267
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_182722"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..793
                     /gene="pspA"
                     /locus_tag="AC16VR_12780"
     CDS             53..793
                     /gene="pspA"
                     /locus_tag="AC16VR_12780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HIR6012078.1"
                     /translation="MSIFSRMSDIINSNINALLDKAEDPEKMVRLIIQEMEEALVEVR
                     STSARAIADKKELARRQEYLDADALEWERKAEVAVAKGRDDLAKGALVERNKAQEAAA
                     AITKDLQILDDTLAKLNDDVTALQAKIKDAKARQNAIIMRGKAAQTRLGVRRQLNDHN
                     IDDAIQRFESYERKMDDLEGQIEAYDMGRKTLADEIAELEVDETLDEELAQLKARMGN
                     ETRSSADAAGAKDAPGREQNCPEHSANG"
     gene            802..1047
                     /gene="pspB"
                     /locus_tag="AC16VR_12785"
     CDS             802..1047
                     /gene="pspB"
                     /locus_tag="AC16VR_12785"
                     /inference="COORDINATES: protein motif:HMM:NF006993.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HIR6012079.1"
                     /translation="MDGLAIAFFVPTVVFLTIVAPIWIFMHYRSKQRAQGALSEDERM
                     ELEALTSQAERMAERIETLESILDSETPGWRSRAQTE"
     gene            1157..1777
                     /locus_tag="AC16VR_12790"
     CDS             1157..1777
                     /locus_tag="AC16VR_12790"
                     /inference="COORDINATES: protein motif:HMM:NF015957.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HIR6012080.1"
                     /translation="MEKLEAALHQLVDTARDQLSDRATAFIEETTATLEREFGRGTGP
                     DRPAAGRRRHGHGHRARDRATEDGIPSRSRRLYRDPGRAKIGGVCAGIARYYGIETWV
                     VRCAAVTGLIFVPQFVFPAYWIAYFLMGLPPREPASGSNGRARDDHSSPAPELGSKLS
                     PRRSLRNIQADLTQIELKLRRMETHVVSGQYELQKELNKIDEVGRG"
     gene            1813..2043
                     /locus_tag="AC16VR_12795"
     CDS             1813..2043
                     /locus_tag="AC16VR_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012081.1"
                     /translation="MDSVFMMVVLIVLISCGAGVANNYLRNQRKRAEADADPEVRTDL
                     DRLRERVEVLEKIVTDEKFHLAKEINRLEREP"
     gene            complement(2018..2716)
                     /locus_tag="AC16VR_12800"
     CDS             complement(2018..2716)
                     /locus_tag="AC16VR_12800"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF015000.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HIR6012082.1"
                     /translation="MTFNRHPPLVIGHRGAAGLKPENTLASFSAALELGVDAVELDVH
                     LVEDALVVIHDSTLDRTTNGIGRLSDCTLDTLRLLDAGGGAQIPLLEEVFDLVPPDVG
                     INVELKGVGTGERLADFLTGRWRPEVLVSSFRRPELAAFRRRCPETNVAPLFSRWHPR
                     IWDVAARFTAWSVNLNIKLATRKRIADAQRRGFRVLVYTVNDLDLARSLVDAGVHGIF
                     TDHPERIRVPVLSD"
     gene            2772..3869
                     /gene="dnaX"
                     /locus_tag="AC16VR_12805"
                     /pseudo
     CDS             2772..3869
                     /gene="dnaX"
                     /locus_tag="AC16VR_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003285342.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
     gene            3951..4316
                     /locus_tag="AC16VR_12810"
     CDS             3951..4316
                     /locus_tag="AC16VR_12810"
                     /inference="COORDINATES: protein motif:HMM:NF023592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau C-terminal
                     domain-containing protein"
                     /protein_id="HIR6012083.1"
                     /translation="MVESLEIGGVARMIAEHSVVGSHQGDRLRLTLDTAHDTLLNSAQ
                     QAALQRALGGKLGSDLLLEIEAGSCAAETPAQRNARIAAERQHQAEQTLKRDGVVQSL
                     LSEFDGTLGDVKPMEDSAG"
     gene            4313..4630
                     /locus_tag="AC16VR_12815"
     CDS             4313..4630
                     /locus_tag="AC16VR_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014421330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HIR6012084.1"
                     /translation="MKGLGDFMKQAQAMQERMQQVQEEIAKLEVVGESGAGLVKVTMN
                     GRHEVRRVHLDDSLLAEAKDVLEDLVAAACNDAVRKVEAAQRDKMAGMASGLGLPLDK
                     FPF"
     gene            4630..5229
                     /gene="recR"
                     /locus_tag="AC16VR_12820"
     CDS             4630..5229
                     /gene="recR"
                     /locus_tag="AC16VR_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015503171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HIR6012085.1"
                     /translation="MGISPLIDRLIGAFQVLPGVGPKGAQRMVLHLLQRDREGARQLA
                     ETLLESLDRVSRCSRCRNLSEEAVCSICRDPRRDDDVLCVVESPADILAIEAAGGFRG
                     RYFVLHGRLSPIDGVGPEELGVDTLQRRVAELEPKEVILATNPTVEGEATAHYISKLL
                     KADVPNISRIAHGVPVGGELEYIDGGTIVHALQGRRSIS"
     gene            5264..>6267
                     /locus_tag="AC16VR_12825"
     CDS             5264..>6267
                     /locus_tag="AC16VR_12825"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:NF013755.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HIR6012086.1"
                     /translation="MASWGDVIGLDTEFQRTDTFFPVPGLYQVISGEDVYFLDPLSID
                     DFSAFADVLHDPAIVKVMHACSEDLELIRHHLGVEPQGIFDTQLAYAFQNSRYSVSYA
                     NLVAALLGHDLGKHATRSNWLRRPLTEEQLHYAWEDVRYLPEVYEALTARLDELGRRS
                     WFADAMAEKGRVQGAHPDEYYRGVKKAWRLDALELGVLKRLCAWRERRAMAEDVPRNR
                     VVWDEHLVEFARLGSLSETDVHERLPGPVARRYASELTAEHREGVSAGLAEPLLARPL
                     SPSESKVSRTLRDLAWHKADTMEFARELLARKRDVEACIRHWLDTGELSPAYSGWRAG
                     "
BASE COUNT         1197 a         1916 c         2105 g         1049 t
ORIGIN      
        1 gattcagcac gtcgacgggg ccggcacagg aaactacaga ggcagatttg agatgagcat
       61 tttttctcgc atgtccgaca tcatcaattc gaacatcaac gccctgctcg acaaggcgga
      121 agatcccgag aagatggtgc ggctcatcat ccaggaaatg gaggaagcgc tcgtcgaggt
      181 gcgcagcacg tcggcccggg caatcgcaga caagaaggag ctggctcgcc gtcaggagta
      241 cctcgatgcg gatgccctcg agtgggagcg caaggccgag gtagcggtgg cgaagggccg
      301 cgatgacctc gccaagggcg cgctcgtaga gcgcaacaag gcacaggaag cggcggcggc
      361 catcacaaag gatctgcaga tcctcgacga cacgctggca aagctgaacg acgacgtcac
      421 cgcgctgcag gcgaagatca aggacgccaa agctcggcag aacgcgatca tcatgcgcgg
      481 caaggcggcg cagacgcgcc tgggcgtgcg ccgtcagctg aacgaccaca acatcgacga
      541 tgcgattcag cgattcgaga gctacgagcg taagatggac gatctcgagg gtcagatcga
      601 ggcctacgac atgggtcgaa aaacccttgc cgatgagatc gccgagctcg aggtggacga
      661 gaccctcgat gaagagctcg cccagctcaa ggcgcgcatg ggaaatgaaa cccgatcatc
      721 cgctgacgcc gcgggagcga aggacgcgcc cgggcgggaa cagaattgcc cggaacactc
      781 tgctaacggt taggagcaac catggacggc ttagctatcg cgttcttcgt ccccacggtc
      841 gtctttctca ccatcgtcgc gccgatctgg atcttcatgc attaccggag caagcagagg
      901 gcccaggggg ccttgtccga ggacgagcgc atggagttgg aggctttgac gagccaggcg
      961 gagcgaatgg cggagcgtat cgaaaccctc gaatcgatcc tcgattccga gacgcctggg
     1021 tggcggtcgc gggcgcaaac ggaataggct gagtggacgg agcgaaggca tgaacgattc
     1081 gaattcaggt ggtggatcct ctcgatcgcc tggcgcaggc cggcagcgcg acggagcctc
     1141 tgattttcag caggccgtgg aaaagctgga agcggcgttg caccagctgg tcgacacggc
     1201 cagggatcag ctgtcagacc gagccactgc cttcatagaa gaaaccacgg ctacgctgga
     1261 gcgagagttc gggcgcggca cagggcccga ccggccggcg gccggaaggc gccgccacgg
     1321 gcacgggcat cgggcgcgtg atcgcgcaac ggaggacggc attccctcgc gatcccgccg
     1381 gctgtaccgg gatccggggc gcgccaagat cggcggcgtg tgcgccggca tcgcgcggta
     1441 ctacggcatc gagacctggg tggtgcgctg cgcggcggtg acggggctca tcttcgtccc
     1501 ccaattcgtg ttccccgcct actggatcgc ctatttcctg atgggtctgc cgccgcgtga
     1561 gcctgcgtcg ggcagtaacg gccgtgcccg cgacgatcat tcttcgccgg ccccggagct
     1621 tggttcgaag ctgtcgccgc gtcgcagcct gcgcaacatc caggcagatc taacgcagat
     1681 cgagctcaag ctgcgccgca tggaaaccca tgtggtttcc ggccagtacg agctgcagaa
     1741 agaactcaac aagatcgacg aggtcggccg tggctgaccc ggccaccgcc aaccctgatc
     1801 cgggggcctg ctatggatag tgtcttcatg atggtggtgc tgatcgtgct gatcagctgc
     1861 ggagcaggcg tggccaacaa ctacctgaga aaccagcgca agcgggcgga ggcagacgcc
     1921 gaccctgagg tccgcacgga cctagaccgt cttcgtgagc gggtcgaggt gcttgagaag
     1981 atcgtcacag acgagaaatt tcacctcgcc aaggaaatca atcgcttaga acgggaaccc
     2041 tgatccgctc ggggtgatcg gtgaagatcc cgtggacgcc tgcgtccacc aggctgcgcg
     2101 cgagatcgag gtcgttgacc gtgtagacga gcacccgaaa accgcgtctc tgcgcgtctg
     2161 cgatgcgctt gcgcgtcgcc agcttgatgt tcaggttgac cgaccaagct gtgaaccttg
     2221 ccgcgacatc ccagattcgc ggatgccatc gactgaacag cggcgccacg ttcgtctcag
     2281 ggcaacgcct gcgaaaggcc gccagttcgg ggcggcggaa tgacgagacc agcacctccg
     2341 gtcgccaccg gccggtcaga aagtcggcca gccgctcgcc ggtccccacc cctttcagct
     2401 ctacgttgat accgacgtct ggcggcacca gatcgaaaac ttcctccagc agaggtatct
     2461 gcgcgccgcc cccggcgtcg aggaggcgta gcgtgtcgag ggtgcagtcg gagagccggc
     2521 cgataccgtt cgtggtgcga tccagggtcg agtcgtggat gaccaccagg gcgtcctcaa
     2581 ccagatgcac gtcgagttcc accgcgtcca cgcccagctc cagcgcggct gagaacgacg
     2641 caagcgtgtt ttccggcttc aggccggcgg cgccgcggtg tccgatcacc agtgggggat
     2701 gtcggttgaa ggtcacgggc gggtccaggg cagtttgggt gtctgtggct tacccactac
     2761 aatgggcgat catggggtat caggtactag cacgcaaatt ccggcctgcc ggcttcgacg
     2821 cgctcgtagg ccaggagcac gtcgtgcgcg ccctgcgaca cgccttggac aacgaccgtc
     2881 tgcatcacgc ctatctcttt accgggactc gtggcgtggg caagacgacc attgcgcgac
     2941 tggtggccaa gagcctgaac tgcgaggccg gcgtttccgc atcaccctgc ggcgagtgct
     3001 cgatctgccg ggagattgcc gagaaccggt tcgtcgacct gatcgaggtg gacgccgcct
     3061 cgagaacggg ggtcggcgac acccgcgagc tcttggagaa cgcccagtac ctgccgacac
     3121 gcggccggta caaggtctac ctgatcgacg aggttcacat gctctcgacg ccggctttca
     3181 acgccctcct gaagacgctt gaggagccgc cggagcacgt gaagtttctg ctggcgacga
     3241 cggacccgaa aaaggtaccg gtcaccgtcc tctcccgctg cctgcagttt cagctgaaaa
     3301 acatctccag cgagcgaatc gccgcctacc ttgccgaggt gctccaggca gaggagatct
     3361 ccttcgagcc cgaggcactt gagaccatcg ccgcggccgc gctgggcagc atgcgggacg
     3421 cgctttccat caccgatcag gccatcgctt acggcggcgg gtctctcggc cacgccgacg
     3481 tggtagccat gcttggcctg gtgggacgcg acgaggtgca cagcctgttg gaagcactgg
     3541 ccagtggcga cgccgagcgg gtgcttgcca tgacggcgga gctggcagag cgaggggcgg
     3601 attttgagga agttctaagc ggcgtgatcg cggcctttca ccgtatggcc gtggagcagg
     3661 cgacgggttc aggagccaac gcgctctttg ggccggaggt ggttcagctc tactaccaga
     3721 ttgcgctcat gggctatcgg gacatgcaca tcgttccgga tccgcgtagc ggtttcgaga
     3781 tgaccctgct gcgtatgatt gcgtttgctc ccgacgaggg ccttctgact caaagcactg
     3841 cgccctcgac gacggccgaa cggggagcaa aggagcggcc ggtgccggaa agcgccgggg
     3901 ggcgcgacgg agtcgagtcg accctggccg acggcacgcc gtggcacgag gtggtcgagt
     3961 cccttgaaat tggcggcgtc gcacggatga ttgcagagca cagcgtggtg ggttcccacc
     4021 aaggggaccg gctgcggctt acgctcgata cggcccatga cacgttgctc aactccgccc
     4081 agcaggcggc gcttcagcgc gccctcggag gcaagttggg atcagatctg ctgctcgaga
     4141 tcgaagcggg aagctgcgcg gcggagacac cggcgcagcg aaacgcgcgc attgcggcgg
     4201 agcgtcagca tcaggccgag cagaccctca agcgagacgg ggtcgtgcag tcgttgctca
     4261 gcgagttcga cggcaccctg ggagacgtca aacccatgga ggattcggca ggatgaaagg
     4321 gctcggtgac ttcatgaaac aggcccaggc catgcaggaa cgcatgcagc aggttcagga
     4381 ggaaatcgcg aagctcgaag tcgtgggcga gagcggggcc ggtctcgtca aggtcacgat
     4441 gaacgggcgg cacgaagttc ggcgcgtcca tctcgacgac agcctgcttg cggaagcgaa
     4501 agacgtgctc gaggacctgg tcgctgccgc ctgcaacgac gcggttcgga aggtggaagc
     4561 ggcccaacgg gacaaaatgg cgggaatggc atccggtctc ggcctgccgc tcgacaagtt
     4621 ccctttctga tgggaatcag tccgctaatc gaccggctga tcggggcctt tcaggtgctt
     4681 cccggagtcg gccccaaggg agcgcaacgc atggtcctgc acctgttgca gcgcgatcgt
     4741 gagggcgccc ggcaacttgc ggagacgctt cttgagtccc tggaccgcgt gtcgcgctgt
     4801 tcacgctgcc ggaacctgtc ggaagaggcc gtctgctcga tctgtagaga tccgcgccgc
     4861 gacgatgacg ttctctgcgt ggtcgagtcg cccgcggaca ttctcgccat cgaggcggcg
     4921 ggcggctttc gcggacgcta cttcgtcctg cacggacggc tgtcccccat cgatggggtg
     4981 gggcccgagg aactcggcgt agacacgttg cagcgcaggg tcgcggagct ggaaccgaag
     5041 gaagtcatac tcgccacgaa ccccacggtg gagggcgaag ccacggcgca ttacatcagc
     5101 aagctgctga aggccgacgt acccaacata tcgcgaatcg cccacggggt gcccgttggc
     5161 ggggagctcg aatacattga cggaggaacg atcgttcatg ccttgcaggg ccgacgcagc
     5221 atcagttagc tggatcgacg acactggcgc tctggtcgac gcggtggcgt cctggggcga
     5281 cgtgatcggt ctggacacgg agtttcagcg cacggacacc ttttttccgg tgcccggcct
     5341 ttatcaggtc atcagcggcg aggacgtgta tttcctcgac ccgctgtcca tcgacgattt
     5401 ctcggcgttt gccgacgtgc tgcacgatcc ggccatcgtc aaggtgatgc acgcctgctc
     5461 cgaggacctc gagctcattc gtcatcacct cggggtggaa ccccagggga tattcgatac
     5521 ccagctggcc tatgcgtttc agaactcgag gtacagcgtg agctacgcca acctggtagc
     5581 ggcattgctg ggacacgatc tgggcaagca cgccacccgg tccaactggt tgcgccggcc
     5641 cctgaccgag gagcagctgc actatgcctg ggaggatgtg cgctaccttc ccgaagttta
     5701 cgaagcgctg accgcgcggc tcgacgaact cggcagacgc tcgtggttcg ctgacgccat
     5761 ggccgagaaa ggtcgggttc agggagccca tccggatgaa tactaccgtg gcgtcaagaa
     5821 ggcctggcgc ctggacgctc ttgagctcgg tgtgctcaag cggctttgcg cgtggcgcga
     5881 gcgccgggca atggccgaag acgtgcctcg caaccgggtg gtctgggacg agcatctggt
     5941 ggaattcgcg cgcctcggat cgctttctga gacggacgtg cacgagcgtc tgccgggccc
     6001 tgttgcccgc cggtacgcct ccgagctcac agccgagcat cgggaaggcg tctcggccgg
     6061 gctcgccgag ccgctgctcg ctcgaccctt gagcccgtcg gagagcaagg tcagccggac
     6121 cctgcgcgac cttgcctggc acaaggccga tacgatggag tttgccaggg aactgctcgc
     6181 ccgcaagcgt gacgtggaag cctgcattcg ccactggctg gacaccggcg agctgtcgcc
     6241 cgcctactcc ggctggcggg ccggctt
//
LOCUS       DBNHQR010000561         3211 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_309023, whole genome shotgun sequence.
ACCESSION   DBNHQR010000561 DBNHQR010000000
VERSION     DBNHQR010000561.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_309023"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="AC16VR_12830"
     CDS             <1..456
                     /locus_tag="AC16VR_12830"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6012087.1"
                     /translation="RNMGAAPNQMWLYHCGPQWTKRLLLTGDTLSGEDAARIGLVLKS
                     VPEAYLESEVEGLADRLSWIDPEMLSTNKRIINVGMELMGAQVLQRLAAENDARAHTT
                     QAARNVFKQIATEGLRSALRDRDAPFGDSRARVIGPETRDEQGRLVPWE"
     gene            complement(487..1092)
                     /locus_tag="AC16VR_12835"
     CDS             complement(487..1092)
                     /locus_tag="AC16VR_12835"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001886.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HIR6012088.1"
                     /translation="MTYLEWRPHPWHGLEPGDLDSGVVNAFIEITPFDTVKYEVDKES
                     GYLRVDRPSRTSSLPPTLYGFVPRTYCGDRVKVLMEGADSGDFDPLDICVISDRLVNR
                     AEVIIPARVVGGLPMLDGGEADDKIIAVHANDEIWRDTNDIGDLPEQYVARLRHYFAS
                     YKTLPGESSTVHVGEPYGRERALRVLHASISDYQEQFPANP"
     gene            1261..2265
                     /locus_tag="AC16VR_12840"
     CDS             1261..2265
                     /locus_tag="AC16VR_12840"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIR6012089.1"
                     /translation="MSTSNTRVLLAKRPVGQATPDCFSVETEQLPALEPGQVRVAVEY
                     ISVDAGTRTMLRGEGFHHQVGLGQTILAGGVGHIVDSAADGWELGQAVRGGLGAQTIA
                     TVTPELIERVDESVAPLSAYLGVLGGSTGVTAWIGMRRVAKPQPGDVFVVSAAAGAVG
                     SIAGQIAKRDGARVVGIAGGPRKVSYLLERLGFDAAIDYKNEDVNARLRELAPDGVNV
                     FFDNVGGPILDAVLDNIAMRARVVICGAISQYDDLDNVTGPSLYLRLAERQSTMEGFA
                     FFHFPESIGPATEELARWLADGALVFDEEILEGIERYPEALGFMFEGGNIGKLLVKAR
                     "
     gene            2325..2942
                     /locus_tag="AC16VR_12845"
     CDS             2325..2942
                     /locus_tag="AC16VR_12845"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR6012090.1"
                     /translation="MSEDFSLREAIYNCRAMRRIRPDPVPEELLVELIDAANQAPSGS
                     NSQRARWIVVRDPAVKRQLADQNRRHAEPYIAADVSNPATPKLKRMLDAVVWQMEHMH
                     EIPALIVACFDYPEKVEGMGVYRSAGSVWPGIQNLLLTARALGLGAAPTTLALRDQDA
                     VRKILKLPETFAALCLIPVGYPMGKFGPVTRKPVSEVMRFDAWND"
     gene            2984..>3211
                     /locus_tag="AC16VR_12850"
     CDS             2984..>3211
                     /locus_tag="AC16VR_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012091.1"
                     /translation="MTRWIAVLLMLLWCRPALGGDIGGTWKNDADPVWIEVVHDDGVA
                     TGTVVRNDRNPAAAGRLLLKDLKPGDEANVWR"
BASE COUNT          570 a          970 c         1099 g          572 t
ORIGIN      
        1 cgcaacatgg gcgcggcgcc gaaccagatg tggctgtatc actgcggccc gcaatggacc
       61 aagcgtcttc tgctgacagg ggacacgctg agcggcgagg atgcggccag aatagggctc
      121 gtgctgaagt ccgtgcccga agcgtacctg gagagcgaag tggaagggct tgcggaccgg
      181 ttgagctgga tcgatccgga aatgctctcc accaacaagc ggatcatcaa tgtgggcatg
      241 gagctcatgg gtgcccaggt gctgcagcgg ctggcggcgg agaacgatgc ccgggctcac
      301 accacgcagg cggcgcgaaa cgtgttcaag cagatagcca ccgaagggct gcgctccgcg
      361 cttcgggacc gggatgcgcc gttcggcgac agccgtgcca gggtcatcgg gccggaaacc
      421 agggacgagc agggaaggct ggtgccctgg gagtgatcag agggcccgca gccgcacccg
      481 gtgggctcag gggtttgcgg gaaactgttc ctggtagtcg gaaatcgacg catgcagaac
      541 ccgcagcgcc cgctcgcgcc cgtacggctc gcccacgtgg acggtcgacg attccccggg
      601 aagggtcttg tacgaggcga agtagtgccg caggcgggca acgtattgct cgggcaggtc
      661 gccaatgtcg ttggtgtcgc gccagatctc gtcgttcgca tgaaccgcga tgatcttgtc
      721 gtcggcctcc ccgccatcga gcatcggtag cccgccaacc acccgtgccg ggatgatgac
      781 ctcggcgcgg ttgaccagcc ggtcactgat cacgcagatg tccagagggt cgaaatctcc
      841 cgagtccgcc ccttccatca gcactttcac acggtctccg caatacgtgc gcggtacgaa
      901 accgtacagc gtcggtggca gcgaggaggt gcggctcggc cggtccaccc gcagataacc
      961 cgactccttg tcgacctcgt atttcaccgt gtcgaaaggc gtgatctcga tgaaggcgtt
     1021 gacgacaccg gagtcgaggt cgccgggctc gagcccgtgc cacgggtggg ggcgccactc
     1081 taggtaggtc actggtcgtt ctgcgttggt tggtgaaggt ggactatatc tgtgcccgcc
     1141 ggtaattggc agatttggcg tcacctttcg atggctgctt tttcgcggct taagacggct
     1201 tctagggacg gcgggatgcg ggacggggcg aaggtgggcg ggctgtgcta ggctcgcgcg
     1261 atgtctactt cgaacacccg tgtcctgctg gcaaagcggc ccgtcggaca ggcaacgcct
     1321 gactgtttct ccgtggagac cgagcagctg ccggccctcg agcctggcca ggtgcgcgtc
     1381 gccgttgagt acatctcggt ggatgccgga acgcgcacca tgctgcgcgg agaggggttt
     1441 catcatcagg tgggactcgg gcagacgata ctcgcgggcg gggttggcca catcgtcgac
     1501 tcggccgcag atggctggga attgggtcag gcagttcgcg gcgggcttgg cgcgcagacg
     1561 atcgcgacgg tcacgccgga attgatcgag cgcgttgacg aatccgtagc acccctgagc
     1621 gcgtatctgg gcgtgctggg gggatccacg ggcgtgaccg cctggattgg catgcggcgc
     1681 gtcgccaaac ctcagccggg ggacgttttc gtggtgtccg cggcggcagg agcggtggga
     1741 tccattgccg gtcagattgc caaacgcgac ggcgcgcgcg tcgtcggaat cgccggcggt
     1801 ccccgaaagg tctcctacct tctcgaacgc ctagggttcg acgctgcgat cgactacaag
     1861 aacgaggacg tcaatgcccg cctgcgcgag ctggcgcccg acggggtgaa cgtcttcttc
     1921 gacaacgtgg gcggccccat cctggatgcc gtgctggaca acatcgcgat gcgggcgagg
     1981 gtcgtcatct gtggagccat ctcgcaatac gacgatctgg acaacgtcac cggcccctcg
     2041 ctctaccttc ggctggccga gcgccagtcc accatggaag ggtttgcgtt ctttcacttc
     2101 cctgagagca ttggaccggc gaccgaggag cttgcgcggt ggctggccga cggcgcgctc
     2161 gttttcgacg aggagatcct ggaaggtata gagcgctacc ccgaggcgct tgggttcatg
     2221 ttcgaaggcg gcaacatagg caagctgctc gtgaaggcgc gttgaccaac ggggtcgccg
     2281 cactttgcac cctcttttga ttcgtaaact cacaggaagc caaggtgtct gaagacttct
     2341 cacttcgcga ggcgatctac aactgccggg ccatgcgccg gatcaggccc gaccccgttc
     2401 cggaggaact cctcgtcgag ctgatcgatg cggcgaacca ggcgccgtcg ggatcgaact
     2461 cccagagggc gcgatggatc gtcgtcagag accccgcggt caagcgtcag ctggcggacc
     2521 agaaccgcag gcacgcagag ccttacattg ctgccgacgt ctctaacccg gccacgccga
     2581 agctgaagag gatgctcgat gccgtcgtat ggcagatgga gcacatgcac gagataccgg
     2641 cgctgatcgt cgcctgtttc gattaccccg agaaggtgga gggcatgggc gtgtaccgca
     2701 gcgccgggtc cgtatggcct ggcatccaga acctgctgct gacagctcgc gctctgggcc
     2761 ttggcgcggc gcccaccacc ctcgcgctcc gcgatcagga tgccgttcgg aaaattctga
     2821 agctgcctga gacctttgcc gcgctctgcc tgatcccggt cggctatccg atggggaagt
     2881 tcgggcccgt cacccgaaag cccgtttctg aggtcatgcg gttcgacgcc tggaacgact
     2941 agcggccggt attttgcccg ccggaaggag gagaaaccga cccgtgacga gatggattgc
     3001 tgttctgctg atgctgctgt ggtgccggcc ggcactcggt ggtgacatcg gcggaacctg
     3061 gaaaaacgac gccgatcccg tgtggataga ggtcgttcat gacgacggcg tggccaccgg
     3121 caccgtggtc cgcaacgacc gcaatcccgc ggcggccggc cggctgctgc tcaaagatct
     3181 gaagccgggc gacgaagcga acgtctggcg g
//
LOCUS       DBNHQR010000562         4524 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1765066, whole genome shotgun sequence.
ACCESSION   DBNHQR010000562 DBNHQR010000000
VERSION     DBNHQR010000562.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4524)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1765066"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="AC16VR_12855"
     CDS             <1..459
                     /locus_tag="AC16VR_12855"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008171464.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HIR6012092.1"
                     /translation="HHAGNSSGVVDGASAVLVVSPDFAKAHGLRPRARIRMTATAGTE
                     PLIMLTAPAPAAQRCLQKAGMTRDDIDLWEINEAFASVVLKFMKEMGVDHDVVNIDGG
                     AIALGHPIGATGGMLIGTAVDQLEREDKETALVAMCTGGGMGTATILERI"
     gene            complement(493..3057)
                     /locus_tag="AC16VR_12860"
     CDS             complement(493..3057)
                     /locus_tag="AC16VR_12860"
                     /inference="COORDINATES: protein motif:HMM:NF023649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HIR6012093.1"
                     /translation="MRAFSSFTAPLLVLLALSAFAQPTTDYLTPDLRARVERLKADVQ
                     QVPTNTTNVAERSRVLWNWANALSLDGGVLPVNLTQAVSFVFAYPDLMADRHDVIDGF
                     IQELTLLDEEPGALGTLRADSGPFQAASFVTLRQTFTVGSKAIQAGGGILVARHFQPN
                     YGAWQTGDPAADNYLSITTSAADASFTTETAPMIGMHGGFRRPADALVFRVASGTLRP
                     GDTVTLTYGDRSQGSRGFLMADFSSDMMPLPLYVAFTGSGRFFSLPIQPVAVIGGPAA
                     GVHAFAPSIVKAGEPFTLAVRAEDRFYNRASDGHPDWLVSLNGEPFREVPASEEPITL
                     LRNIEIAEPGVYRFTIASRDGQISGRGNPILVKQTAEERIFWGDTHGHSGFAEGIGTP
                     DFFMRWARDDARLDFVTHSEHDIWLDDGEWEQLRNNARSYTREGEFVAFLGYEWTVRN
                     THGGHHNVLFRTPAGRNRLAAPFYPSLSKLYAGLRGDAQPEDVLVIPHAHQAGDYRTS
                     DPELEHLVEVNSQHGNFEWFGQAYLRHGHQVGFTAASDNHLSQPGYGAPKNGTLAQRG
                     GLGAVIARARTTDALFDAMKQARAYATSGERIILEFSVNGTPMGQRGPFSETRTIEGR
                     VIGTAPIDDISLLRNGEEIWRRELLTDAADNTSSPGAFLLTFASDSDPRQPGDNPRGW
                     RHWRGTIEVRDATLIDASGMDFNNQLLQQIEADAGDPDLLRFSTITRGDTSSIRLDLE
                     DVSRATQVRVTLEPTQETGGAPATYGRHEQLPAAEVKLEIRDFENGETRAQLPAGPRE
                     DTITLRRIITQGSMDEAFTIDDVSGRQGDYYYVRVRQADDSLAWSSPVWIGGHAPR"
     gene            complement(3140..3985)
                     /locus_tag="AC16VR_12865"
     CDS             complement(3140..3985)
                     /locus_tag="AC16VR_12865"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019579154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA dioxygenase family protein"
                     /protein_id="HIR6012094.1"
                     /translation="MSFAVHPKPRGFGAAVTGIRLSDPISASLADELRAAWLAHQVLF
                     FPDQPMSHEDLERFTLCFGAFGNDPYVKAIPGHPHILEVRREPGENVAPFGASWHSDW
                     SFQAQPPAATILHAKIVPPVGGETWYADGYAAYEALPATLRDEISELTAIHSARRPYS
                     HQGFLAGRGPERSMTILPSDEALQTQDHPLVRTHPETGRKALWVNGVYTIGIRNMAEE
                     DAKNLLKRLFDHAIRDEFVYRHRWAPDMLTMWDNRSVQHCARGGYDGYRRVMHRTTVA
                     GDSPV"
     gene            complement(3982..4188)
                     /locus_tag="AC16VR_12870"
     CDS             complement(3982..4188)
                     /locus_tag="AC16VR_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012095.1"
                     /translation="MRYLVQLIIPVLIIVTVMFVVARNRRRRDEAGEQSDSEKGMFIF
                     ILVIGALVTVAIVVALQSFWGAGA"
     gene            4291..>4524
                     /locus_tag="AC16VR_12875"
     CDS             4291..>4524
                     /locus_tag="AC16VR_12875"
                     /inference="COORDINATES: protein motif:HMM:NF012686.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family transporter"
                     /protein_id="HIR6012096.1"
                     /translation="MAWVADHWVAAVLFAVYTALLFYNANVGRRRSHDLTDYYVGGRG
                     MGGVVVGISFFATFASTNSYIGHAGKGYEYGLPW"
BASE COUNT          751 a         1397 c         1571 g          805 t
ORIGIN      
        1 caccacgccg ggaactcctc gggcgtggtt gacggcgcca gcgccgtgct cgtcgtgagc
       61 ccggattttg caaaggctca cggtctgcga ccccgagccc gcatccgcat gacggccacc
      121 gccggaaccg agccgctcat catgttgacc gcaccggctc ctgcggccca gcgttgcctg
      181 cagaaggcgg gcatgacgcg ggacgacatc gatctgtggg aaatcaacga ggcgtttgct
      241 tccgtcgtgc tcaagttcat gaaggagatg ggggtcgatc acgacgtggt caacatagac
      301 ggtggcgcca tcgcgttggg tcatcccatc ggcgcgacgg gcggcatgct catcggcacg
      361 gcggtggatc agctcgagcg cgaggacaag gaaaccgcgc tggttgcgat gtgcacaggc
      421 ggaggaatgg gtacggctac gatcctcgag cggatctgag gtcggctccg gcggggtatc
      481 ccaaggcgcc gctcagcgcg gcgcgtgccc gccgatccac accgggctag accacgccag
      541 gctgtcgtcg gcctggcgga cgcgcacgta gtagtagtca ccctggcgcc cgctgacgtc
      601 gtcgatggtg aaagcttcgt ccatgcttcc ctgggtgatg atccggcgaa gggtgatggt
      661 gtcctcccgt ggaccggcgg gcagctgcgc ccgggtctcg ccgttctcga aatctcttat
      721 ctccagcttc acctccgcgg ccggcagctg ctcatggcgg ccgtacgtgg ccggcgcgcc
      781 gccggtttcc tgggtgggtt ccagggtaac gcggacctgc gtggctctgg atacgtcctc
      841 caggtcgagc cgaatgctgc tcgtgtcccc gcgggtgatg gtcgagaaac gaagcaggtc
      901 cggatcccct gcgtccgctt cgatctgctg gagaagctga ttgttgaaat ccatgccgct
      961 ggcatcgatg agcgtcgcgt ccctcacctc gatggttcca cgccagtggc gccaccctcg
     1021 cggattgtct cccggctgtc gcggatcgga gtcggaggcg aaggtcagca ggaaagctcc
     1081 cggagagctc gtattgtcgg cagcgtcggt cagcagttcc cggcgccaga tctcctcgcc
     1141 gtttcgcagc aacgagatgt cgtcgattgg cgccgtgccg atcacccgac cttcgatcgt
     1201 gcgggtttcg gagaagggtc cgcgctgccc catgggcgtc ccgttcaccg agaactccag
     1261 gatgatgcgc tcgccggaag tcgcataggc gcgagcctgc ttcatggcgt cgaacagcgc
     1321 atcggtggtc ctggcccggg caatgacggc gcccagaccg cctcgctggg caagtgtccc
     1381 gttcttgggg gcaccgtatc ccggctggct gagatggttg tctgacgcgg cggtgaagcc
     1441 cacctggtgc ccgtggcgca gataggcctg accgaaccat tcgaagttgc cgtgctggga
     1501 gttgacctcc acgaggtgct cgagttccgg gtcgctggtg cggtagtcgc cggcctggtg
     1561 cgcgtggggg atgacgagca cgtcctctgg ctgggcatca ccgcgcaggc ctgcgtaaag
     1621 ctttgacagg gacgggtaga agggcgctgc cagccgattg cggccggcgg gcgtgcggaa
     1681 cagcacgttg tggtgtccgc cgtgggtatt ccggaccgtc cactcgtagc cgaggaaggc
     1741 gacgaactcg ccttcccggg tgtagctgcg ggcgttgttg cgcagctgct cccactcgcc
     1801 atcgtccagc cagatgtcgt gctccgaatg ggtgacgaag tccaggcgcg catcgtcgcg
     1861 cgcccagcgc atgaagaagt cgggcgtgcc gataccctcc gcgaatcccg agtggccgtg
     1921 ggtatcgccc cagaatatcc gctcctcggc cgtctgcttg accaggattg gatttccacg
     1981 tccgctgatc tggccgtccc ggctcgcgat ggtgaaccga tatacgcctg gttcggctat
     2041 ctcgatgttg cgcaacaggg tgatcggctc ctcgctggcc ggcacctccc ggaacggttc
     2101 gccattgagg gaaaccagcc agtccggatg tccgtccgag gcgcggttgt agaagcggtc
     2161 ctcggcccgg accgccaggg tgaagggctc tccggccttc acgatagacg gcgcgaaggc
     2221 gtgcacgccg gccgccgggc cgccgatcac cgccacgggt tgaatgggta gcgagaagaa
     2281 gcgtccggat ccggtgaacg ccacgtacag gggcaacggc atcatgtcgg aggaaaaatc
     2341 ggccatcagg aatccgcggc tcccctggct gcggtcaccg taggtcaggg tcacggtatc
     2401 gcccgggcgc agcgtcccgg aggcaacgcg aaagacgagc gcgtcagccg gacggcggaa
     2461 accgccgtgc atgccgatca tcggggcggt ttcggtggtg aaactcgcgt cggcagcgct
     2521 ggtggttatt gacaggtagt tgtccgcggc tggatcgccc gtctgccacg cgccgtagtt
     2581 gggctggaaa tgcctggcca ccagaatgcc tccgcccgcc tgtatcgcct tggaaccgac
     2641 ggtgaacgtc tgcctcagcg tgacgaagga cgctgcctgg aagggtcccg aatcggctcg
     2701 aagggttcca agcgccccgg gctcctcgtc gagcagggtc aactcctgga tgaagccgtc
     2761 gatgacgtcg tgccgatcgg ccatcaggtc cggataggcg aacacgaaag agacggcctg
     2821 ggtcaggttg accggcagca caccaccgtc gagagacaag gcattggccc agttccaaag
     2881 cacgcgggat cgctccgcga cgttggtggt gttggtcggt acctgctgga cgtcggcctt
     2941 cagccgttcg acgcgcgcgc gcagatcggg cgtcaggtaa tccgtggtgg gctgcgcgaa
     3001 agccgacagg gcgagcagca cgagcagggg cgcggtgaac gaactaaagg cgcgcaagcg
     3061 tcagggctcc actgtcactg gaccggggcg agcttctcac atggcggcgt gggagggtag
     3121 cgcaatcgcc ggccgggcct cagaccggcg agtcgccggc cacggtggtg cggtgcatga
     3181 cccggcgata cccgtcgtat ccgcccctgg cgcaatgctg aaccgagcgg ttgtcccaca
     3241 tggtcagcat gtcgggcgcc cagcggtgac ggtagacgaa ctcgtcccta atggcgtgat
     3301 cgaacaaccg cttcagcaga ttctttgcgt cttcctccgc catgttccga atgccgatgg
     3361 tgtagacgcc gttcacccac agggccttgc ggccggtttc ggggtgcgtg cgaacgagcg
     3421 gatggtcctg ggtctgcagt gcttcgtcgc tgggcaggat ggtcatgctc cgctccgggc
     3481 ctctcccggc caggaatccc tggtggctgt aggggcggcg ggcgctgtgg atcgcggtga
     3541 gctcggagat ctcgtcgcgc agggttgccg gcagggcctc gtaagccgcg tagccatccg
     3601 cgtaccaggt ctcgccgccc accgggggga cgatcttggc gtgcagaatg gttgcggccg
     3661 gcggctgggc ctggaaggac cagtcggagt gccaggacgc cccgaaaggg gcgacgttct
     3721 ccccaggctc gcgcctgact tccaggatgt gcggatgacc ggggatggct ttcacgtagg
     3781 ggtcgtttcc gaaagcgccg aagcacagag taaatcgttc caggtcctcg tggctcatgg
     3841 gctggtcggg gaagaagagc acctgatggg ccagccaggc cgctctgagc tcatcggcca
     3901 gggaggcgct gatcgggtcg gaaagccgga taccggtaac ggcggcgcca aacccccgcg
     3961 gcttaggatg cacggcgaag ctcatgcccc ggccccccag aaggattgca aggcgacgac
     4021 gatggccacc gtcaccaggg caccgatgac caggatgaag atgaacatcc ccttctcgct
     4081 gtctgattgc tcccctgcct catcgcggcg gcggcggttg cgggcgacca cgaacatcac
     4141 cgtcacgatg atcagaaccg ggatgatgag ctgtaccagg taacgcatgg acggattccc
     4201 gcggcttgtg gcgttcgagc ataacgagac ggtccgccag cgatcaatgg tacgctttgg
     4261 ccgggtcagg ccgggaaagg tagtgttcgc atggcgtggg tcgccgacca ttgggttgct
     4321 gccgtgcttt ttgccgtcta cacggcgctg ctgttctaca acgccaacgt ggggcgcagg
     4381 agaagtcacg acctgacgga ttactacgtc ggcggtcgcg gtatgggcgg cgtcgtggtg
     4441 ggcatttcct tcttcgcgac ctttgccagc accaacagct acatcggtca tgcgggcaag
     4501 ggctacgagt acggtctgcc gtgg
//
LOCUS       DBNHQR010000563         2161 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1639483, whole genome shotgun sequence.
ACCESSION   DBNHQR010000563 DBNHQR010000000
VERSION     DBNHQR010000563.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1639483"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="AC16VR_12880"
     CDS             <1..922
                     /locus_tag="AC16VR_12880"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6012097.1"
                     /translation="GVVLEELGKALAVTPYLSSVVLGGGAVDLGGSAEQKAEILPGVC
                     DGTTLLTLAYQETARHDPYAIETSAVKTDGQWVLNGRKQLVLDGGSADRIVVAARSSG
                     APGDRSGLTLFLVDAGAAGLDVKPGLLLDSRSAAHVALNDVTVGEDAVIGEVDGGAEI
                     LDRVFERAAVALSAEMLGGIQEAFDRTVEYLKEREQFGAKIGSFQGLKHRAARWFCEV
                     ELTRSIVMQALRAIDDGAENLAEIVSTCKARASDAFRLSGQEGIQMHGGMGVTDEADI
                     GFFLKRARVSELLLGDSAFHRNQFAELKGY"
     gene            939..1364
                     /locus_tag="AC16VR_12885"
     CDS             939..1364
                     /locus_tag="AC16VR_12885"
                     /inference="COORDINATES: protein motif:HMM:NF013920.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HIR6012098.1"
                     /translation="MTQVSINDNVLELSAEGPRLKGMRCKTCGNHVFPFQKGCPKCAG
                     TDVEPVDLDTRGTLWAWTIQGFPPKAPPYLGEADPEKFKPYGVGYVEIPGQTKVEARL
                     TESDPTKLKEGMEMELTVEPISKDEDGNDIVTYAFKPVE"
     gene            1376..>2161
                     /locus_tag="AC16VR_12890"
     CDS             1376..>2161
                     /locus_tag="AC16VR_12890"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012336.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR6012099.1"
                     /translation="MSDVAIVGIGMHPFGRHEGVTGMEQGASAVREACRDAGVEWKDI
                     QFAFGGSSAAGAPDTMVSQLGLTGLQFINVQNGCATGGSALFSAYNTIMSGAYDLGIA
                     IGFDKHERGAFRIQSGSGKGRDWYGGSGMALTTQFFGMKINHYMQQYGISQSTLAKVA
                     SKAFKNGSMNPMAWRRQALSEEEVLQSQMLSYPLTQYMFCSPGEGGVALILARADQAH
                     KYTKTPVYLKAAVVRSRRFGSFEVLAPSLALEHNAGPTIDASKA"
BASE COUNT          391 a          663 c          748 g          359 t
ORIGIN      
        1 cggcgtggtg ctcgaggagc tcggcaaggc gctggcggtg acgccctacc tgtccagcgt
       61 ggtgctaggc ggcggcgccg tagacctcgg cggcagtgcc gagcagaagg ccgagatcct
      121 tcccggcgtc tgcgatggca cgacgctgct gacgctcgcc taccaggaga ccgcccgtca
      181 cgatccctac gccatcgaga cgagcgccgt gaaaaccgat ggccagtggg tgctcaacgg
      241 ccgcaagcag ctggttctgg acggcggcag cgcggaccgg atcgtggtcg cggcaaggtc
      301 gagcggggcg cccggcgatc ggtcggggct gaccctgttc ctggtcgacg cgggcgccgc
      361 cgggctcgac gtgaagccgg gcctgctgct cgacagccgc agcgcggctc acgtggccct
      421 gaacgacgtg accgtgggcg aggatgccgt gatcggcgag gtcgacggtg gtgccgagat
      481 tctcgaccgg gtcttcgagc gcgcggcggt ggcgctctcg gccgagatgc tcggaggcat
      541 ccaggaagcc ttcgaccgga ccgtcgagta tctcaaggaa cgcgagcagt tcggcgcgaa
      601 gatcggttcc tttcagggct tgaagcaccg cgcggcccgc tggttctgtg aggtggagct
      661 gacccgctcc atcgtcatgc aggcgctgcg cgccatcgat gacggcgctg aaaacctcgc
      721 cgagatcgtg agcacctgca aggcgagggc ttcggatgcc ttccggcttt cgggccagga
      781 agggatacag atgcacggcg gcatgggcgt gacggacgag gcggacatcg gcttcttctt
      841 gaagcgcgcc cgggtctcgg agctgctgct cggggactcg gccttccatc gcaaccaatt
      901 tgctgagtta aagggctatt agcccgactg gaggtgacat gacccaggtt tccatcaacg
      961 acaacgtgct ggagctgtcg gcggaagggc cgcggctcaa aggcatgcgc tgcaagacct
     1021 gcggcaacca cgtttttccg tttcagaagg gctgcccgaa gtgcgcgggc accgacgtgg
     1081 agcccgtgga tctagacacc cgcggcacgc tgtgggcctg gacgattcag ggctttccgc
     1141 ccaaggcccc tccctatctg ggtgaagccg atccggaaaa gttcaaaccc tatggcgtgg
     1201 gctacgtcga gattcccggg cagaccaagg tcgaggcccg gttgaccgag tcggacccga
     1261 cgaagctcaa ggagggcatg gagatggagc tgacggtgga gcccatcagc aaagacgaag
     1321 acggtaacga catcgtcacc tacgcattca aaccggtcga gtaaggagag aaaccatgag
     1381 cgacgtagca attgttggca taggcatgca cccattcggc cggcacgagg gcgtgaccgg
     1441 gatggagcag ggtgcgtcag cggtccgcga agcttgcagg gatgcgggcg ttgaatggaa
     1501 ggacattcag ttcgccttcg gcggcagctc ggcggccggc gcgccggaca cgatggtctc
     1561 gcagctcggt ctcacgggtt tgcagttcat caacgtccag aacggctgcg cgactggcgg
     1621 gtcggcgctc ttctccgcct acaacaccat catgtccggg gcttacgatc tcgggatcgc
     1681 catcggcttc gacaagcacg agcgcggggc gtttcggatt cagagcggct ccggcaaggg
     1741 gcgtgactgg tacggcggca gcggcatggc gctgacgacc cagttcttcg gcatgaagat
     1801 caaccactac atgcagcagt acggcatctc gcagagcacg ctcgccaagg tcgcttccaa
     1861 ggccttcaag aacggctcca tgaaccccat ggcctggcgg cgccaggcgc tgtccgagga
     1921 ggaggtgctg cagtcccaga tgctctccta cccgctgacc cagtacatgt tctgctcccc
     1981 gggcgagggt ggcgtggcat tgatactcgc tcgtgcggat caggctcaca agtacacgaa
     2041 gacgcccgtc tacctgaagg cggccgtggt gcgctcgcgg cgtttcggca gcttcgaggt
     2101 gctggcgccg tcccttgcgc tcgagcacaa cgccgggccg accatcgatg cgtccaaagc
     2161 c
//
LOCUS       DBNHQR010000564         2403 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_878817, whole genome shotgun sequence.
ACCESSION   DBNHQR010000564 DBNHQR010000000
VERSION     DBNHQR010000564.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_878817"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="AC16VR_12895"
     CDS             <1..225
                     /locus_tag="AC16VR_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012100.1"
                     /translation="EFAGERMPFLPRDVLTLPVRNVTVEELAPFLLERLGSDPEIRTL
                     ELQRIELRVSSGPGQWAAATWTAGQEIREV"
     gene            229..924
                     /locus_tag="AC16VR_12900"
     CDS             229..924
                     /locus_tag="AC16VR_12900"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6012101.1"
                     /translation="MKILLITGASAGIGLHTAQRFVAEGFAVVNLSRRRCPIDAVSQI
                     NCDLSVPGFIDNISNQLSGMLQEADRVVLVHNAARMGNDTAVETPSVALREALEVNVV
                     APNTLNYFTIPYMKPGSAIIYVGSTLAEKAVPGTYTYVVSKHALIGMMRSTCQDLAGQ
                     SIHTACVCPGFTDTEMLRQHVPAEAMASIRAMSAYGRLIDPDEIAETIFWAASNPVIN
                     GAVLHANLGQRES"
     gene            924..1730
                     /locus_tag="AC16VR_12905"
     CDS             924..1730
                     /locus_tag="AC16VR_12905"
                     /inference="COORDINATES: protein motif:HMM:NF014631.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0160254 - tRNA queuosine(34) biosynthetic
                     process from salvaged queuosine or its precursors
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HIR6012102.1"
                     /translation="MQGGVRTAGGNEETSGNRLSEQDLNERRERVFIVLAGFFLCAMT
                     LLNVVGITRFIQLGPMALAVGVLPYPLTFLCTDLISELYGRKRANFLVWVGFTLNFFI
                     LGMLWLGSSIPAVDPATQPPWQVIELAEEIALPNGDVLSGSVELFQLLYACTSGAVFA
                     SMMAYIAAQFCDVQLFHFFKRLTKGRHLWLRNNFSTLTSQMVDSFMVISVTFGAAYFA
                     GSITLAAMLVLMGSNYLFKMTVALADTLPFYYLTGRLKRYLQIDNVHVLE"
     gene            complement(1773..>2403)
                     /locus_tag="AC16VR_12910"
     CDS             complement(1773..>2403)
                     /locus_tag="AC16VR_12910"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6012103.1"
                     /translation="GYAPVMLRAFDVRPSEKRELFERSLALMRQAWRGEPVVLDDGET
                     TVTLDPLPVQRPHPPLWVAAFGPKALAQAGRLGLPYLASPVETLDTLEANYRRHGEAA
                     AEAGHGPRDTIPVMRTVFVSSNPRETRELLRRMNAEAEKSGRLAPGAEAHEWTIVGDD
                     QFVRDRIVEYRERLGVNHLIVTRLRLGGVDEGAVQASVGRVADIVGGLP"
BASE COUNT          413 a          808 c          756 g          426 t
ORIGIN      
        1 gagttcgccg gcgagcgcat gccctttctg ccacgggacg tcctcaccct tcccgtgcgc
       61 aacgtcacgg tagaggaact ggcgccgttt ctgctggagc gtctcggctc ggatcccgag
      121 atccgcacgc tcgagctgca gaggattgag ctgcgcgtct cttcaggacc cggccagtgg
      181 gctgcggcga cctggaccgc cgggcaagag atacgggagg tgtaagccat gaagatactg
      241 ctcattaccg gagccagcgc ggggatcggg ctgcacactg cccaacgctt cgttgccgaa
      301 ggattcgccg tggtgaacct ctcgcggcgc cgctgtccca tcgacgcggt cagccagatc
      361 aactgcgacc tgtcggttcc cgggttcatc gacaacatca gcaatcagct cagcggaatg
      421 ctgcaggagg ccgaccgggt ggtgctcgtg cacaacgccg cccggatggg caacgacacg
      481 gcggtggaga ccccgagcgt ggccctgcgc gaggcgctgg aggtgaacgt ggtcgcaccg
      541 aacacgctca actacttcac catcccgtac atgaaaccgg gctcggccat catctacgtg
      601 ggctccaccc tggcggagaa ggcggtaccc ggcacgtata cctatgtggt gagcaagcac
      661 gccctgatcg gaatgatgcg ctcgacctgc caggatctgg cggggcagag catccatacc
      721 gcgtgcgttt gcccgggctt cacggatacc gagatgctgc gtcagcacgt tccggccgaa
      781 gccatggcga gcatccgcgc catgtcggcc tacggacggc tgatcgaccc ggacgagatc
      841 gccgaaacca tcttctgggc cgccagcaac ccggttatca acggggccgt tctgcacgcc
      901 aatctgggtc agcgggaaag ctgatgcagg gaggcgtaag aacagcgggg ggcaacgagg
      961 aaacgtcggg aaaccgcctc agcgagcagg acctgaacga gcgacgcgag cgggtgttca
     1021 tcgttctggc cggctttttc ctctgcgcca tgacgctgct aaacgtcgtc ggcatcaccc
     1081 gtttcataca gctgggcccc atggccctgg cggtgggcgt gctgccctac ccgctcacct
     1141 tcctgtgcac ggatctcatc agcgagctgt acggccgcaa acgcgccaac ttcctggtct
     1201 gggtgggctt caccctgaac ttcttcattc tcggcatgct ctggctcggc agctcgatac
     1261 cggccgtaga tcccgccacg cagccgccct ggcaggtcat cgagctggcc gaggagattg
     1321 cgctgcccaa cggcgacgtc ctcagcggca gcgtggagct gtttcagctg ctctatgcct
     1381 gcacctcggg cgccgtgttc gcttcgatga tggcctacat cgccgcgcag ttctgcgacg
     1441 tgcagctttt tcacttcttc aagcggctga ccaagggtcg acacctgtgg cttcgcaaca
     1501 acttttccac gctgacgagc cagatggtgg actccttcat ggtcatcagc gtcactttcg
     1561 gcgcagccta cttcgccggc agcatcacgc tggcggccat gctcgttctg atgggcagca
     1621 actacctctt caagatgacg gtggcgctgg cggacaccct gcccttctac tacctcacgg
     1681 gtcgcctcaa gcgctacctg cagatcgaca acgttcacgt cctggagtga ccggcccgat
     1741 cggatcgctc cggcgtgatc cggtggaggc agtcagggca gaccgccgac gatgtccgcc
     1801 acccgcccga cggaagcctg cacggcacct tcgtccaccc cgccgagcct gagtcgcgtc
     1861 acgatcaggt gattgacccc gagccgctcg cggtactcga cgatccggtc cctgacgaat
     1921 tggtcgtctc cgacgatggt ccactcgtga gcttccgcgc cgggcgccag gcgaccggat
     1981 ttttccgctt ccgcattcat ccggcggagc agctcccgtg tctctctcgg gttggacgaa
     2041 acgaataccg tgcgcatcac cgggatcgta tcgcgaggcc cgtggccggc ctccgctgcc
     2101 gcctcgccgt gacgccgata attggcttcg agcgtatcga gggtctccac gggcgacgcg
     2161 aggtaaggca gcccgagacg gcccgcctgg gcgagtgcct tcggcccgaa ggcggcgacc
     2221 cacagcggcg ggtgcgggcg ctgaaccggc agcggatcca gggtgacggt ggtctcgccg
     2281 tcatccagca cgaccggctc accgcgccac gcctgccgca tgagggctaa ggagcgctcg
     2341 aagagctcgc gcttctcgct gggccggacg tcgaacgctc gaagcatgac cggcgcgtac
     2401 ccc
//
LOCUS       DBNHQR010000565         3955 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1448578, whole genome shotgun sequence.
ACCESSION   DBNHQR010000565 DBNHQR010000000
VERSION     DBNHQR010000565.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1448578"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="AC16VR_12915"
     CDS             <1..535
                     /locus_tag="AC16VR_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine amidotransferase-related protein"
                     /protein_id="HIR6012104.1"
                     /translation="LGICYGLHAAVIDYARHVCGLDGAHSTEIDAETPHPVIALITEW
                     LDRDGQIEQRTSETDLGGTMRLGEQVCVLKDGTIAQRMYGAETVRERHRHRYEVNNQY
                     IERLEAAGLVVAGRSEDGELVEMIELSGHPWFVACQFHPEFTSTPRAGHPLFSGFIGA
                     ASDRAAAAPVLRVGTSD"
     gene            551..1396
                     /gene="kdsA"
                     /locus_tag="AC16VR_12920"
     CDS             551..1396
                     /gene="kdsA"
                     /locus_tag="AC16VR_12920"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF003543.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HIR6012105.1"
                     /translation="MRLCDFEVGLDRPLFLIAGPCVIESEQLALETAEALKALTDELA
                     VAFVYKSSFDKANRSSHESFRGPGLEAGLRILERVNREVGVPVLTDVHEDTPLDEVAA
                     VVSVLQTPAFLCRQTDFIQRVCSQGLPVNIKKGQFLAPADMIHVVTKARATGNEQILV
                     CERGASFGYNNLVSDMRGLAIMRATGCPVVFDATHSVQMPGGLGAASGGQREMVPVLA
                     RAAVAAGISGIFMETHPDPDQALSDGPNSWPLSLMAPLLRQLVDLDRVAKERPFLEDQ
                     YAQGR"
     gene            1400..2683
                     /gene="eno"
                     /locus_tag="AC16VR_12925"
     CDS             1400..2683
                     /gene="eno"
                     /locus_tag="AC16VR_12925"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIR6012106.1"
                     /translation="MSRIAAVIGREILDSRGNPTVQADVVTDDGFMGSACAPSGASTG
                     SREALELRDGDASRYLGRGVLNAVGAVNGEVAKALAGVDPADQEAIDALLCELDGTPN
                     KGRLGANGLLAVSLAAAKAAAAAKNQALFEHINDLFGGVAMRMPLPMMNILNGGAHAD
                     NNVDIQEFMVQPVSMGSFREGLRCGAEVFHALGRLLRSRGLTTSVGDEGGFAPDLASN
                     AHALAVVAEAVSDAGYELGKDVTLALDCAATEFYADGQYRLAGEHRDLSSEEFAAYLE
                     DLTARYPVVSIEDGMDENDWDGWRALTDRVGDRIQLVGDDLFVTNTEILAEGIRRGVA
                     NAILIKLNQIGTLSETLSTIRMAQEAGYRTVISHRSGETEDTTIADLAVATGAGQIKT
                     GSLSRSDRVAKYNRLLRIEEMLGDAAEYRGRGELL"
     gene            2688..2969
                     /locus_tag="AC16VR_12930"
     CDS             2688..2969
                     /locus_tag="AC16VR_12930"
                     /inference="COORDINATES: protein motif:HMM:NF016839.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HIR6012107.1"
                     /translation="MATRLLIVVLAVLVALLQYRAWFGEVGYFAAQELADEVDEQRRR
                     GAELARRNELLTAEVVALKTGVDALEARARKDLGMIKRGETFYLIPDDD"
     gene            2992..3924
                     /gene="truD"
                     /locus_tag="AC16VR_12935"
     CDS             2992..3924
                     /gene="truD"
                     /locus_tag="AC16VR_12935"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF013322.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HIR6012108.1"
                     /translation="MAVPGKRAKARVKATPEDFLVDELRAAAPSGAGEHLYLHVEKRL
                     LETRQVARSLAELYGVAAVDVGYAGMKDKRAVARQWLSVRTSLGPEVAERGGVVVLAA
                     GRDERKLRRGELAGNRFEIRLCEVSGDDWRDRLGRVFESGAPNYFGPQRFGGDNLDRA
                     LAWLPVRRRRSISPFRRGLYLSVLRSLLFNAVLARRVQEKTWRALLPGDVDLDGMPTA
                     PLWGRGRSATSGVALAAESAALAPFADVLDGLEHAGVDQARRSLVLLPQNAWVAEEDE
                     AVRLGFELPPGGYATSLLREAFELGEATDGAPAS"
BASE COUNT          667 a         1223 c         1393 g          672 t
ORIGIN      
        1 tctgggaatc tgctacggtc tacatgccgc cgtgatcgac tacgcccgcc acgtctgcgg
       61 attggacggt gctcattcca ccgagataga cgccgagacg cctcacccgg tcattgccct
      121 gatcaccgaa tggctggatc gggacggcca gatagagcag cgcaccagcg aaaccgacct
      181 cggcggcacg atgcggcttg gcgagcaggt gtgtgttctc aaggacggca ccatagccca
      241 gcggatgtac ggcgccgaga ccgtccgaga acggcaccga caccgctacg aagtgaacaa
      301 ccagtatatc gagcggctgg aagccgccgg actggtggtg gcaggacgct cggaagacgg
      361 cgagctggtg gaaatgatcg agctgagcgg ccatccgtgg ttcgtagcct gccagttcca
      421 cccggaattc acgtcgaccc cgcgggccgg acaccccctg ttttcgggct tcatcggggc
      481 agcgagcgac cgcgccgccg cggctccggt gctgagagtc ggcacgtccg actagcaagg
      541 accccccctc atgcgcctct gcgatttcga ggttggtctg gaccggccgc tcttcctcat
      601 tgccggtccc tgcgtgatcg agtctgagca gctggcactg gagaccgcgg aggcgttgaa
      661 agcgctcacc gacgagctag ccgttgcctt cgtctacaaa agctcctttg acaaggccaa
      721 ccgttcgtct cacgagagtt ttcgcgggcc cgggctcgag gccgggttgc ggattctgga
      781 acgcgtcaac cgcgaagtgg gcgtacccgt gctgaccgac gttcacgaag acaccccgct
      841 cgacgaggtg gccgccgtgg tttcggtgct gcagacgccg gcctttctct gccggcaaac
      901 agacttcata cagcgggtct gcagccaggg gctgcccgtt aacatcaaga agggtcagtt
      961 tctcgcccct gcggatatga ttcacgtggt cacgaaggcg cgcgcaacgg gcaacgagca
     1021 gatcctggtc tgcgagcggg gcgcgagctt cggctacaac aacctggtct cggacatgcg
     1081 cgggctcgca atcatgcgag ccacgggctg ccccgttgtc ttcgacgcga cccactcggt
     1141 gcagatgccg ggcggcctgg gcgctgcctc gggggggcag cgggagatgg ttccggtgct
     1201 ggccagagcg gcggtggcgg ccgggatatc ggggatattc atggagaccc atcccgatcc
     1261 cgatcaagcg ctatccgacg ggcccaattc ctggcccctg tcgctgatgg cgcccctgct
     1321 ccgacagctg gtggacctgg accgcgtggc gaaggagagg ccgtttctcg aggaccaata
     1381 tgcacaagga agataggaca tgagcagaat tgcggcggta attggccgcg agattctcga
     1441 ttcgcggggt aaccccacgg tgcaggccga cgtcgtcacc gacgacggct tcatgggctc
     1501 cgcctgcgct ccctccgggg cgtccacagg gtccagggag gcgctcgagc tgcgcgacgg
     1561 cgatgcatcc cggtatcttg gcaggggcgt gctgaacgcg gttggggccg taaacgggga
     1621 ggttgccaag gcgttggcag gcgtcgaccc ggccgaccag gaggcgatcg acgcactgct
     1681 ttgcgagctc gacgggacgc caaacaaggg caggctcggc gcaaacgggt tgcttgcggt
     1741 gtccctggcg gccgcgaaag cggcggcagc ggccaagaac caggcgctct tcgagcacat
     1801 caacgatctc ttcggtggtg tcgcgatgcg catgccgctg cccatgatga acatcctcaa
     1861 tggcggcgcc catgcggaca acaacgtgga catccaggag ttcatggtgc agccggtctc
     1921 catggggtcg ttccgcgaag ggctgcgctg cggggcagag gtatttcacg ccctggggag
     1981 gctgctgcgc tcgcggggcc tcaccacctc ggtcggggac gaaggcggct tcgctccgga
     2041 cctggcctcg aacgcgcatg ctctggccgt ggtcgccgaa gcagtgagcg acgccgggta
     2101 cgagctcgga aaggacgtaa cgctcgcgct ggattgcgct gccacggagt tctacgcgga
     2161 cggtcagtac cgtctggccg gcgagcatcg tgacctgtcc agcgaggagt tcgccgccta
     2221 tctcgaagac ctgactgcgc gctatccggt ggtctccatc gaggacggca tggacgagaa
     2281 cgactgggac ggctggcgcg cgctgaccga tcgggtcggc gatcggattc agctcgtggg
     2341 cgacgatctg ttcgtcacga acacggagat ccttgccgag ggcatacgcc ggggggtggc
     2401 aaacgcgatc ctgatcaagc tcaaccagat cgggactctg agcgaaaccc tcagcacgat
     2461 cagaatggct caagaggcag gatatcgcac cgtgatatcc caccggtcgg gtgaaacgga
     2521 ggacaccacc attgccgatc tggccgttgc caccggcgcg ggccagatca agaccggctc
     2581 cctgagccgt tcggatcggg tggcaaaata caaccgcctg ctgcgcatcg aggagatgct
     2641 cggcgatgcc gctgagtacc gcggtcgcgg cgagctgctc tgagcccatg gcgacgcggt
     2701 tgctcatcgt cgttctggcc gttctggtcg ccctcctgca gtaccgggca tggttcggcg
     2761 aggttggtta cttcgccgcg caggagctgg cggacgaggt ggacgagcag cgccgccggg
     2821 gtgcggagct ggcgcggcgc aacgagctgc tgacggcgga agtggtggcg ctcaagacgg
     2881 gcgtagacgc gctcgaggcc cgggcacgca aggatctggg catgatcaag cggggtgaaa
     2941 ctttttatct gattccggat gacgattgat ccgcatttcg gccgtctgcc cttggccgta
     3001 ccgggaaagc gggccaaggc ccgtgtcaag gcgactccgg aagacttcct ggtcgacgag
     3061 ctgcgagccg cggcgccgag cggcgcgggc gagcatctct acctccacgt ggagaagcgc
     3121 ctcttggaaa cgcggcaggt ggcccgtagt ctggccgagc tttacggggt cgcggccgtg
     3181 gatgtgggct atgccggaat gaaggacaag cgcgccgttg cccgtcagtg gttgagcgtg
     3241 cgcacgtctc tcggccccga ggtggcggaa cggggcggcg tggtcgtgct tgccgcaggt
     3301 cgtgatgagc gcaagcttcg tcggggtgag ctcgccggaa accggttcga gatccggttg
     3361 tgcgaggtgt ccggtgacga ctggcgcgac cgcctcgggc gcgtcttcga gtcgggagcg
     3421 ccgaactact tcgggcccca gcgttttgga ggcgacaatc tcgatcgcgc cctagcctgg
     3481 cttcccgtac gccgccgccg gtccatctcg ccgttcagga ggggtctcta cctgtccgtg
     3541 ctccgcagcc tgctcttcaa cgcggttctg gcccggcggg tgcaggagaa aacctggcgg
     3601 gcactgctcc cgggcgacgt ggatctggac ggaatgccca cagcccccct ctggggccgg
     3661 ggtcggtctg cgaccagcgg cgtggccctg gcggcggaga gcgccgcgct cgccccgttc
     3721 gcggatgtgc tagacggcct cgagcatgcg ggggtggatc aggccaggcg atccctggtg
     3781 ctgttgccgc agaacgcctg ggtggcggag gaagacgagg ctgtgcgcct cggcttcgaa
     3841 ctgccccctg gcggatatgc gaccagcctg ctgcgcgagg ctttcgagct gggcgaggcc
     3901 acggatggag caccggcgtc gtgacgggct acgatctggt cggcatcggc atgac
//
LOCUS       DBNHQR010000566         4524 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1324026, whole genome shotgun sequence.
ACCESSION   DBNHQR010000566 DBNHQR010000000
VERSION     DBNHQR010000566.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4524)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1324026"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="AC16VR_12940"
     CDS             <1..417
                     /locus_tag="AC16VR_12940"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF016267.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HIR6012109.1"
                     /translation="ILQDVDLDAMRRFACRLAGKAITGGHEVHIHTQSAEDASIVDEL
                     LWSYPDDRFIPHETLDDSGRAAVAAPVTLGWTEPDGLDGVLINLSDEIPRFFGRFDRV
                     AEVIPRSRRDAGRDRYKFYRDRGYPLFHHELDDWEA"
     gene            417..902
                     /locus_tag="AC16VR_12945"
     CDS             417..902
                     /locus_tag="AC16VR_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012110.1"
                     /translation="MSTKDSADSEDELLGDLESLRSILDEEDELMPQDDEAVPLLDDV
                     VADAADAEEPARASPGGLDDDLFQALLSDDWRESAAAIIEQAPAALGQAVAWKPREIA
                     ALRQALRLKQDETLNQWISDALERHAEALQAELLEAFSGELKPAVEGILRRDAGGEHG
                     E"
     gene            892..3666
                     /locus_tag="AC16VR_12950"
     CDS             892..3666
                     /locus_tag="AC16VR_12950"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010952166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HIR6012111.1"
                     /translation="MESSFDPHAIEQKLYADWEGADYFAPRGNGEGYCIAIPPPNVTG
                     SLHMGHAFQHTLMDALIRYERMKGRRTLWQMGTDHAGIATQMLVERQLLAEGLKREDI
                     GRDAFVERVWDWRNHSGNNISKQLRRMGSSLDWSRDRFTMDEGFCHAVLEVFVRLYEE
                     GLIYRGQRLVNWDPELKSVISDLEVENTEERGHLWHFRYPLAGGLTTREGKPYLVVAT
                     TRPETMLGDTAVAVHPDDERYAALVGGSVILPLADREIPIIADEYVDRDFGTGCVKIT
                     PAHDFNDNEVGARHGLALINVFNEDATINEAAPQPYRGLDRFDAREKVVADLKSQGLL
                     DGIEDHTLMVPRGDRSNAIVEPLLTHQWFVKIQPLADPAIKAVENGDVEFVPKQYENV
                     YFSWMRNIQDWAISRQQWWGHRIPAWYDAEGNVYVGRDEDEARRNGGLADDVPLDQDP
                     DVLDTWFSSALWTFVTLGWPEETEELSQFHPTDVLVTGHDIIFFWVARMIMMTLKFTG
                     EVPFRQVYVHGLVRDADGKKMSKTKGNGLDPLDLIDGVSLDELIDKRTQNLTQPQMAP
                     SIEKNTRADFPEGIPSYGTDALRFTYCALASTGRDVRFDLSRIEGYRNFCNKLWNAAR
                     FVLMNCPDDEEPGRRDLSLADRWIVSEMRSFLETSERAINTYRFDLYANAVYEFVWHE
                     YCDWYLELTKPLLWDEQADPSLLRGTRYTLLSVLDTLLRAAHPIMPFITETIWREVAP
                     RLGNTNPTIMLQPFPEPADLPSDPDAEAAVEWLKGVIVGIRNIRGEAGIKPAQEISIL
                     LQGGSDADRALAQVAESLLKRLAKVDHIDWLADGAEPPPNALALVGELRVMVPLAGLI
                     DVAAERARLAKEVEKKSQELNRIEAKLANENFVAKAPQAVVEKERAKAGDARAALATL
                     EQQLASLDDL"
     gene            complement(3649..>4524)
                     /locus_tag="AC16VR_12955"
     CDS             complement(3649..>4524)
                     /locus_tag="AC16VR_12955"
                     /EC_number="1.6.5.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - quinone reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HIR6012112.1"
                     /translation="VHATAVNRADLVQRSGGYPPPPGASKILGLECAGEVLAIGEGVT
                     RVKPGDRVCALLAGGGYAERVAVPAGQVLPVPSGLDMLQAAALPEVFATAYLNLYTEA
                     RLERGERVLIHAGASGVGTAAIQLCSTTSNPCFVTVGNPQKIERCVALGAEGGWDRHQ
                     GSFLEAVRQWGGVDVVLDPVGAGYLADNLSCLNIDGRLSLIGLMGGVQSEINLATVVM
                     KRIRIIGSTLRARSAAAKSLVMDGLQRDVWPHLEAGSIVPIIDEVLPIESAERAHELM
                     AGDQTFGKLVLQIVE"
BASE COUNT          861 a         1515 c         1447 g          701 t
ORIGIN      
        1 atcctccagg acgtcgatct ggacgccatg cgcagattcg cctgccgtct tgccggcaag
       61 gccatcacgg gcggccacga ggtccacata catacccaga gcgccgagga tgccagcatc
      121 gtggacgagc tgctctggtc gtaccccgac gaccgcttca tcccccacga gaccctcgac
      181 gactccggcc gcgcggccgt cgccgcgccg gtgactctgg ggtggaccga gcccgacggt
      241 ctggacggcg tgctcatcaa cctgagcgac gagatcccgc gcttcttcgg ccgcttcgac
      301 cgggtcgccg aggtgatccc gcggtcacgc cgggacgcag gacgggatag atacaagttc
      361 tatcgggacc gcggatatcc gctgttccac cacgagctcg acgactggga agcgtgatga
      421 gcaccaaaga ctctgcagac tccgaggacg agctgctggg cgacctcgaa tccctgcgtt
      481 cgattctgga cgaggaggac gagctgatgc cccaggacga cgaggccgtt ccgcttctgg
      541 acgacgtggt cgcagatgcg gccgacgcgg aggagcccgc ccgggccagt ccgggaggat
      601 tggacgacga cctgttccag gcgctgctga gcgatgactg gcgcgaatcg gccgccgcca
      661 tcatcgagca ggcgcccgct gccctcggcc aggccgttgc ctggaagccc cgggagatcg
      721 cggcccttcg gcaagccctg cgtctgaagc aggacgaaac cctgaatcag tggataagtg
      781 atgcactcga gcgccacgcc gaagcgctgc aagccgagct gctcgaagcc ttctccggtg
      841 agctgaagcc cgcggtagaa ggcatcttgc gtcgcgacgc ggggggcgaa catggagagt
      901 agtttcgatc cccacgccat cgagcagaaa ctgtatgccg attgggaagg cgcggactat
      961 tttgcgcccc ggggcaatgg cgaaggctac tgcatcgcca tccccccacc caacgtcacg
     1021 ggcagcctcc acatggggca cgcgttccag cacaccctga tggacgcgct catccgctat
     1081 gagcgcatga aggggcgacg cacgctgtgg cagatgggta cggatcatgc gggaatcgcc
     1141 acccagatgc tggtcgagcg gcagctgctg gcagaggggc tgaaacggga ggacatcggc
     1201 cgggacgcct tcgtcgagcg agtctgggac tggcgcaacc actccggcaa caacatctcc
     1261 aagcagctgc gccgcatggg ttcgtctctc gactggtccc gcgaccggtt caccatggac
     1321 gagggcttct gccacgccgt gctcgaggtg ttcgtgcgcc tctacgagga agggctcatc
     1381 taccggggcc agcgcctggt gaactgggac ccggagctga aatcggtcat ttcggatctg
     1441 gaagtcgaga acaccgagga gcgcgggcac ctctggcact tccgctatcc gctcgccggc
     1501 ggcctgacaa cccgcgaggg caagccgtac ctggtggttg caactacccg acccgaaacc
     1561 atgctcggtg acacggccgt cgccgtgcac cccgacgacg agcgctacgc cgctctggtg
     1621 ggcggctcgg tgattctgcc gctggcggac cgggaaattc ccatcatcgc cgacgagtac
     1681 gtcgatcgcg acttcggcac cggctgcgtg aagatcacgc cggcccacga tttcaacgac
     1741 aacgaggtgg gcgcacgaca cgggctcgcg ctgatcaacg tcttcaacga ggacgccacc
     1801 ataaacgagg ccgcgcccca gccctaccgc gggctggacc ggttcgacgc tcgcgagaaa
     1861 gtggtggcgg acctgaaaag ccaggggctc ctggacggga tcgaggacca tacgctgatg
     1921 gttccccggg gagaccgctc gaacgcgatc gtcgagccgc tgctgactca ccagtggttc
     1981 gtgaagatcc agccgctggc agacccggcc atcaaggctg tcgagaacgg tgacgtcgag
     2041 ttcgttccaa agcagtacga gaacgtctac ttctcctgga tgcgcaacat ccaggactgg
     2101 gccatctcgc gccagcagtg gtggggccac aggatccccg catggtacga cgccgaaggc
     2161 aacgtctacg tgggccggga cgaagacgaa gcccgccgca acggcggcct cgccgacgac
     2221 gttcccctgg accaggaccc ggacgtcctg gacacctggt tctcctccgc gctgtggacg
     2281 ttcgtgacgc tgggctggcc ggaggagacc gaggagcttt ctcagtttca ccccacggac
     2341 gtgctcgtca cgggtcacga catcatcttc ttctgggtgg ccaggatgat catgatgacg
     2401 ctgaagttca ccggcgaggt gccgttccgg caggtctacg tgcacgggct ggtgcgcgac
     2461 gccgatggaa agaagatgtc caagaccaag ggcaacggcc tggatcccct agacctgatc
     2521 gacggggtct ctctggacga gctgatcgac aagcgaaccc agaatctcac ccaaccgcag
     2581 atggccccga gtatcgaaaa gaacacccgg gccgatttcc ctgaaggcat tccgtcctac
     2641 ggaacggacg ccctgcgttt cacctactgc gccctggcaa gcaccggccg cgacgtgcgt
     2701 ttcgacctca gccgcatcga gggataccgc aacttctgca acaagctctg gaacgcggcc
     2761 cgtttcgtgc tcatgaactg ccccgacgac gaagagccgg gcaggcgcga tctgagcctg
     2821 gccgaccgct ggatcgtctc cgagatgcgc tctttcctgg agacgtccga acgcgccatc
     2881 aacacctacc gcttcgacct ctatgccaac gcggtgtacg agttcgtctg gcacgaatac
     2941 tgcgactggt atctggagct gacgaagccg ctgttgtggg acgagcaggc cgacccgagc
     3001 ctcctgcgcg ggacgcgcta cacgctgctg agcgtgctgg acacgctgct gcgcgcagcc
     3061 cacccgatca tgccgttcat caccgagacc atctggcgcg aggtggcccc gcggctgggc
     3121 aacaccaacc cgaccatcat gctgcaaccg tttccggagc ctgcggacct gccgtcggat
     3181 ccggacgcgg aagcggccgt cgagtggctc aagggcgtca tcgtcggcat ccgcaacatc
     3241 cgcggagaag ccggcatcaa gccagcgcag gagatcagca ttcttctgca gggcggcagc
     3301 gacgcggatc gggcgctcgc tcaggtggcg gaatcgctgc tcaagcggct ggcaaaggtc
     3361 gaccacatag actggctggc cgacggcgcg gaaccgccac caaacgctct ggcgctcgtc
     3421 ggcgagctga gagtgatggt ccccctggcc ggcctgatcg acgtggctgc ggaacgcgcg
     3481 cggctcgcca aggaagtgga gaaaaagtcc caggagctca accgcatcga ggcgaagctg
     3541 gccaacgaga atttcgtcgc caaggccccg caggcggtgg tggaaaagga gcgggccaaa
     3601 gccggcgatg cacgcgccgc gctggccacg ctcgagcagc agctcgcctc actcgacgat
     3661 ctgtaacacc agcttaccga acgtctgatc gcccgccatg agctcgtgag cccgctccgc
     3721 ggattcgatg gggagcacct cgtcgattat cggaacgatg gaccccgctt caagatgcgg
     3781 ccagacgtcc cgctgcagac cgtccatgac cagcgacttg gccgccgccg agcgcgccct
     3841 gagcgtggag ccgatgatgc gaattcgctt catgaccacg gtggcgaggt tgatctcgga
     3901 ctgaacccca cccatcaaac cgatcagcga gagccggcca tctatgttga gacaggaaag
     3961 gttgtctgca aggtagccgg ctccgaccgg atcgagcacg acatcgacgc cgccccactg
     4021 cctgacggct tcgaggaaac tgccctggtg ccgatcccag ccgccctcgg cccccaaagc
     4081 cacgcagcgt tcgatcttct gaggattgcc cacggttacg aagcagggat tgctcgtggt
     4141 gctgcacagc tgaatggccg cggtaccgac gccgcttgcc ccggcatgga tgagcacgcg
     4201 ctcaccgcgc tccagacgcg cctccgtgta aaggttgaga taagcggtgg caaatacctc
     4261 cggcagggcg gccgcctgca acatgtccag cccggacggc accggcagga cctgccccgc
     4321 agggaccgcg acgcgctccg cgtagccgcc cccggccagg agcgcgcaga cccgatcgcc
     4381 gggctttacc cgggtcacgc cttccccaat ggccagcacc tctcccgcgc attccaggcc
     4441 caggatcttg ctcgctcccg gtgggggcgg gtagcctccc gagcgctgca ccaggtccgc
     4501 acgattgacg gccgtcgcgt gcac
//
LOCUS       DBNHQR010000567         2730 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_689813, whole genome shotgun sequence.
ACCESSION   DBNHQR010000567 DBNHQR010000000
VERSION     DBNHQR010000567.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_689813"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1697)
                     /locus_tag="AC16VR_12960"
     CDS             complement(<1..1697)
                     /locus_tag="AC16VR_12960"
                     /inference="COORDINATES: protein motif:HMM:TIGR03623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012113.1"
                     /translation="MSPIAPLVVTPTARLARTLSRRVALERAAGGAGAWVPPRIQSFP
                     AWLANQREDYFLTATDHRVPIGAHQALWLWQSLIDRQVFIGDPEVAALAQRAWRLIHE
                     HALEAPERWPELMLSEDARAFRGWAAAYRTECDRRGVVDEWAFAGELPGLIEAGHLAI
                     PQGLELAGFDLPMTPLQVRIFDALEAMGCAVSGRPEGSASRTSQTAAMELIRFHLPDD
                     ELRGAAGWARSVLEAEPGRTVAVVVPDLAGRLERVESIFREVFDPPASVLGPRGAEPF
                     HVSLGRRLSEWSLVADALAFLELGSQRLTQPQAGRLLRSPFLAGWPDETLDRSRALAR
                     LVSRAPFEVTVNELRWALDGVGAAALDEALQSWQTLREAAAQSAWPSDWAGRFQQELS
                     ALGFASGRPLDSREYQVLNRWHDLLESFGALDVVAAGPWSRTQALRQLAERARSSVFR
                     ERNPGVPVEVLGVEEALGSSFDAVWITTLDSATWPGSPVREPLIPAAVQASVPRASGD
                     GCLARAQLELDALCASAPDVRGSYARGVEDQALEITGLLRHLPVIDAPPPTPAPGAR"
     gene            complement(1771..2412)
                     /locus_tag="AC16VR_12965"
     CDS             complement(1771..2412)
                     /locus_tag="AC16VR_12965"
                     /inference="COORDINATES: protein motif:HMM:NF018717.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HIR6012114.1"
                     /translation="MSRPAYMIIAIDVRDPSRMAEYAGGTQPLLEAAGARVLAATNGI
                     TVEDGVWARERIALLEFPSMESARSFWSADAYAPLKALRESISTSDVVLVEGTQDERV
                     GEEDGAHYLLGASTMLNADWVEEYIQKVPPISARFGVKMLAGGPEFETLEGAWPGESV
                     VVLRFPSEHAFREFWYGDDYRPMKELREANAPGHHISFAETFELPDAGGPSQG"
     gene            2520..>2730
                     /locus_tag="AC16VR_12970"
     CDS             2520..>2730
                     /locus_tag="AC16VR_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012115.1"
                     /translation="MAEAIPTREIQSWPEQALRGHRIPGSRYTSKEFAEKEWEGMWTK
                     VWLLLGREEELPNPGDWQCEEVGPES"
BASE COUNT          509 a          952 c          896 g          373 t
ORIGIN      
        1 aaacgtgccc cgggcgccgg cgtcggcggg ggcgcgtcaa tgaccggcaa atggcggagc
       61 aggccggtga tctccagcgc ctgatcctct acgccccggg catagctgcc gcggacatcg
      121 ggggccgacg cgcaaagcgc gtccagctcc agctgggccc tggccaggca tccgtctccg
      181 ctcgcccgcg gcacggatgc ctgaacggca gccggtatca gcggttcgcg aaccggcgaa
      241 cccggccacg tcgcgctgtc caaggtggtg atccaaaccg catcgaagct ggagccgagc
      301 gcctcctcga cgccgagaac ctccacgggc acgccggggt tgcgctcacg aaagacgctg
      361 gatcgggcac gctctgcaag ctgtcgcagg gcttgcgtac gggaccaggg cccggcggcc
      421 accacgtcca gagcgccgaa actctccagc agatcgtgcc agcgattgag cacctgatac
      481 tcgcggctgt ccaggggccg ccccgaggcg aacccgagag ccgacagctc ctgctgaaat
      541 cggcccgccc aatccgacgg ccatgccgac tgagcagccg cctcgcgcag cgtttgccag
      601 ctctggagcg cctcatcgag ggctgccgca ccgaccccgt cgagcgccca gcgcagctcg
      661 ttgacggtca cctcgaaggg tgcccgggaa accaggcgcg ccagcgcccg ggaccgatcg
      721 agcgtctcgt cgggccagcc ggcgagaaag ggagagcgca gcaatcggcc cgcctgaggc
      781 tgggtgagcc gctggctgcc cagctccaga aaggccaggg cgtcagcaac cagggaccat
      841 tccgagaggc gtctgcccag ggaaacgtga aacggctcgg cacctcgcgg accaaggact
      901 gatgccggcg ggtcgaacac ctcccggaaa atggactcca cccgctcgag ccgtcccgcc
      961 agatccggga ccaccacggc gaccgttcgc cccggctccg cttccagcac agatcgcgcc
     1021 cagcccgccg cgccgcgcag ctcgtcgtca ggaagatgaa agcggatgag ctccattgcc
     1081 gccgtctggg aagttcggga cgcagaccct tcggggcgtc cggagaccgc gcagcccata
     1141 gcctcgagcg catcgaagat gcggacctgc agcggcgtca tcggcagatc gaagccggcc
     1201 agctccagtc cctgcgggat cgccaagtgg cccgcctcga tgagtcccgg cagctccccg
     1261 gcgaacgccc attcgtccac cacaccgcgc cggtcacact cggtacggta cgcggcagcc
     1321 cagccgcgga acgcccgtgc gtcctccgac agcatgagct cgggccagcg ctccggcgct
     1381 tccagggcgt gctcgtggat gagccgccat gcccgctgcg ccagcgccgc cacctcgggg
     1441 tcgccgatga acacttggcg atcgatgagc gactgccaca gccacagcgc ctgatgggcg
     1501 ccgatgggaa cgcgatgatc ggtggcggtg aggaagtaat cctcgcgctg attggccagc
     1561 caggccggga agctctgaat gcgcggcggc acccaggcac cggccccgcc ggccgcccgc
     1621 tccagagcca cgcgacggga aagcgttcgg gcgagcctgg cggtgggcgt cacaacgagg
     1681 ggcgcaatcg ggctcacccg tgcctcaccc gatcaaggcg agccgaacgg ctcgatcgga
     1741 cagtacccgg ccaagggcgg ggagaccgcc tcaaccttgg gatggcccgc cggcgtcggg
     1801 cagctcgaag gtttccgcaa aagagatgtg atgccccggg gcgttggctt cgcgaagctc
     1861 cttcatcggg cggtagtcat caccgtacca gaactcccgg aaggcgtgct cggaagggaa
     1921 ccgcaacaca accacggact cgccgggcca ggcaccttcg agggtctcga attccgggcc
     1981 tccggccagc atcttgacgc caaagcgggc cgagatggga ggcaccttct gaatgtactc
     2041 ttccacccag tctgcgttca gcatggtgct cgccccgagc aggtagtggg caccgtcctc
     2101 ctctccaacc cgctcgtcct gcgtaccctc gaccaggacc acgtccgacg tgctgatgga
     2161 ttctcgcagc gccttcagcg gcgcgtacgc atcagcgctc cagaaggacc tggcgctctc
     2221 catcgagggg aactcgagga gggcgattcg ctcccgagcc cagacaccgt cttcgacagt
     2281 gatcccgttg gtcgcggcga gcaccctggc gccggcggct tccagaagcg gctgtgtgcc
     2341 gcccgcgtac tcggccattc gtgacggatc cctgacatca atggctataa tcatgtatgc
     2401 cggtcggctc atgggctgac tccctgacgc gctctttaag ttgcacatca taagggaaag
     2461 tgaacgcgtt atactcggca gcccggagcg aacaaaacgt cagagcgaga gaaagcacaa
     2521 tggccgaagc aattccaacc cgcgaaattc aaagctggcc cgagcaggcg ctgcgcgggc
     2581 accgcatacc aggatcccgc tacacctcga aggagtttgc cgagaaagag tgggaaggca
     2641 tgtggaccaa ggtctggctg ctgctgggcc gggaagagga gctgccgaac cccggcgact
     2701 ggcagtgcga ggaggtgggc cccgagtcca
//
LOCUS       DBNHQR010000568         1927 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_752770, whole genome shotgun sequence.
ACCESSION   DBNHQR010000568 DBNHQR010000000
VERSION     DBNHQR010000568.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_752770"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="AC16VR_12975"
     CDS             complement(<1..322)
                     /locus_tag="AC16VR_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012116.1"
                     /translation="MLERIRNLSLLRVATIYVVGGWIVIQFAEIALEAFEVPIFVMQW
                     VMAIVFSGFPLVILALSMMGVPFRRSAAEEVEERDESREESAQPQQPVDPGDLPARDN
                     RPSIA"
     gene            329..493
                     /locus_tag="AC16VR_12980"
     CDS             329..493
                     /locus_tag="AC16VR_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012117.1"
                     /translation="MTFKKLGGKTGSGFQQRSESEGYRRIRHDATPARRPFSQHVQTR
                     ETGPVARDIV"
     gene            564..1001
                     /locus_tag="AC16VR_12985"
     CDS             564..1001
                     /locus_tag="AC16VR_12985"
                     /inference="COORDINATES: protein motif:HMM:NF018717.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HIR6012118.1"
                     /translation="MEVINQLYPTRKDQIEKMMEPGPDGPIVMVNLLKFREKAEYEDG
                     RETDLTGREAYMLYGAAVSKLITEYGGRLVFAGDVTNLTIGEVEELWDEIALAEYPNR
                     AALVAMSTSEAYAKIAAHRTAGLAGQLNIETTYQAGLTQPLRG"
     gene            1152..1901
                     /locus_tag="AC16VR_12990"
     CDS             1152..1901
                     /locus_tag="AC16VR_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012119.1"
                     /translation="MRKILTSIAAATLLFGISLSAAAAPGWLATGLTVMPENAPKVVA
                     ALDALFDSKVGRKLPGRVILRANLADGNNPETHAIVSLFRSAAESEAYRAELWADPAW
                     GEFMAAMRGLNETAGSTMRGMIAYNSGERADSDVVWVNHYVTVSEPAVLLSAMRSYSQ
                     SATGQAAPGQVYLSAVTAGGVGSASHIISVGWASEAEWESEVQRTAGNPQWNALIRTM
                     RAVAEYHGATLQRDIKSWGKASVEEITNLGN"
BASE COUNT          409 a          613 c          610 g          295 t
ORIGIN      
        1 gggcaatcga cggtcgattg tcgcgcgccg gaaggtctcc aggatccacc ggctgttgag
       61 gctgtgcgct ctcttcccgg gactcgtcac gctcctcgac ttcctcggcg gcggaccggc
      121 gaaagggcac gcccatcatg ctcagggcga gaatcaccag gggaaatcct gaaaaaacga
      181 tcgccatgac ccactgcatg acgaaaatgg gcacctcgaa cgcttcaagg gcgatttctg
      241 caaactggat gacgatccag ccgcccacca cgtagatcgt cgcgacccgc agcagactga
      301 gattccggat tcgctctagc acttcgtcat gacgttcaag aaattagggg gcaagaccgg
      361 gagtggattt cagcagcgtt ccgaatccga aggctaccgc agaatacgcc acgacgcgac
      421 gccggcacgg cgccccttca gccagcacgt tcagaccagg gaaaccggcc cggttgcgcg
      481 tgacatcgtg taatgattac cattatttca acgcgccccg cggtcgctat ggagcccgag
      541 ggtatcagcc agaggaaccc gatatggaag tgatcaatca gctctacccc acccgaaagg
      601 accagatcga gaagatgatg gagccggggc ccgatggtcc catcgtcatg gtcaacctgc
      661 tgaagttccg ggaaaaggcg gagtacgaag acggccgcga aacggacctg accggacgcg
      721 aggcctacat gctctacggc gcggcggtat cgaagctcat caccgagtac ggtggccggc
      781 tcgtcttcgc aggggacgtg accaacctga ccatcggcga ggtggaggag ctgtgggacg
      841 agatcgccct ggcggagtac cccaatcgcg ccgccctggt ggccatgtcc acctccgagg
      901 cctacgcgaa gatcgccgcc caccgcaccg ccggcctggc gggccagctc aacatcgaga
      961 cgacttacca ggcgggcctt acccagccac tgcgggggta gccctggcgc atgcatgcag
     1021 ggcaacggac gcttcgggga acggataccc cggcccaggg gcgaaaagac gggccaaacg
     1081 tcgctctcgt tcgctatagt gaccggcacc tgcctcggga aggggcagat caataaagga
     1141 aaatactaac catgagaaag atcctgactt caatcgccgc agcaactctg ctgttcggaa
     1201 tcagcctcag cgcggccgcc gcaccgggat ggctcgccac cgggctgacg gtcatgcccg
     1261 agaacgcccc caaggtggtg gcggccctgg atgcgctgtt cgactccaag gtcggacgca
     1321 agctgcccgg ccgcgtgatc ctgcgggcaa acctggcaga cgggaacaat ccggaaaccc
     1381 acgccatcgt atcgctcttc cgatcagcgg ctgagagcga ggcctatcgc gccgagctct
     1441 gggccgaccc cgcctggggg gagttcatgg ccgcgatgcg cgggctgaac gagaccgcag
     1501 gcagcacaat gcggggcatg atcgcctaca acagcggcga gcgagcagac tccgacgtcg
     1561 tctgggtgaa tcactacgtg acggtttccg agccggccgt tctgctgagc gccatgcgca
     1621 gctacagcca gtcggcaacc ggccaggccg ctccgggtca ggtgtatctc agcgcggtca
     1681 ctgccggcgg ggtggggtcg gcttcccaca tcatctccgt cggctgggca agcgaggctg
     1741 aatgggagag cgaggtccag cgcaccgccg ggaatcctca atggaatgcg ctaatcagaa
     1801 ccatgcgggc agtggccgag taccacggcg ccaccctgca gcgggacatc aagtcctggg
     1861 gcaaagcgtc tgtcgaagag atcaccaatc tcggtaactg aagtccgcct tcggcgcccg
     1921 gcgcatg
//
LOCUS       DBNHQR010000569         3283 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_183639, whole genome shotgun sequence.
ACCESSION   DBNHQR010000569 DBNHQR010000000
VERSION     DBNHQR010000569.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_183639"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1522)
                     /locus_tag="AC16VR_12995"
     CDS             complement(<1..1522)
                     /locus_tag="AC16VR_12995"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HIR6012120.1"
                     /translation="MRTDIAREVWEARYRFYRSGEPVDQTIEDTWQRVADTIASRERE
                     PGRWASAYRDLLGDFCFLPGGRILAGAGTGRRVTLCNCFVMGTIEDAMEPIFTALKEG
                     ALTMQAGGGVGYDFSTLRPVGTPARETGNTASGPVSFMHIWDTMCATLCSTGARRGAM
                     MATLRCDHPDIHAFVDAKRDPDALTHFNCSVLVTDAFMNALRDDAEWPLVFPAHHLPT
                     DQGTDIIRREWPGEDGAVPCAVVRRVRARELWEQIMRATYDCSEPGVLFIDRINHENN
                     LSYCERLSATNPCGEVPLPAYGACDLGSFNLTSFVRDPFAAGAAFDFDALRRRVPEAV
                     RFLDSVIDVSGYPLPQQAEQARASRRIGLGFTGLGDALIMLGLHYGSDAARELAAKVM
                     TCIRHEAYAASVALAREKGPFPLFQADAYLQSPGIQRLPESLRRDIARDGIRNSHLTA
                     VAPTGTISLLANNVSSGIEPVFAAEYRREVRGADGTSAVRTVRDFACQLWRDTGRSE"
     gene            complement(1537..2061)
                     /locus_tag="AC16VR_13000"
     CDS             complement(1537..2061)
                     /locus_tag="AC16VR_13000"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR6012121.1"
                     /translation="MSESQISVESAKPAIRAEQAEPAEESKGLATAPRERDMERLFED
                     FFDKRWLRPWSREWPGFESILEKKGPKVDVVDREHEIFVRAELPGMSKDDIEVSVSDD
                     SLSIKGSSHKEEETEEGDYHRREISTSYVARTVSLPARVRGDDAKATLRDGMLEVTIP
                     KAEDSKRRRVEVES"
     gene            2276..3109
                     /locus_tag="AC16VR_13005"
     CDS             2276..3109
                     /locus_tag="AC16VR_13005"
                     /inference="COORDINATES: protein motif:HMM:NF026066.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR6012122.1"
                     /translation="METASREDVNLWVAGSLKEMASLLEDQAANPFRINAYRRAARTL
                     EGLDEPVDALYRRSGREGLMALPSIGEGIATAIENLLRTGRLPQLERLRGSVEPETVF
                     DSLPGVGPRLAAAIHDTLHVDTLEELEVAAHDGRLQTVPGIGPSRAASIRALLAERLK
                     RPPRRALARAPSVAAILDADGQYRREAAAGRLRTIAPRRFNPSHKAWLPVLHTDRNDW
                     HMTLLFSNTARAHELGKTDDWVVVYYYDGDHNEGQCTVVTETAGPRRGARVVRGRENE
                     A"
BASE COUNT          544 a         1081 c         1109 g          549 t
ORIGIN      
        1 cctcgctcct gccggtgtcg cgccacagct ggcaggcgaa gtcacggacg gttcgaacgg
       61 cggaggtgcc atcggcgccg cgcacctcgc gccggtactc ggcggcgaag acgggctcga
      121 tgccgctgga gacgttgttc gccagcaggc tgatggttcc ggtgggcgcg acggcggtca
      181 ggtggctgtt gcggatcccg tcccgggcga tgtcgcggcg gagactttcg ggcagtcgtt
      241 ggatgcccgg cgattgcagg tacgcgtcag cctgaaacaa aggaaagggc cccttttccc
      301 gggccagcgc cacgctggcc gcgtaggctt cgtgtcggat gcacgtcatg actttcgctg
      361 ccagctcgcg cgcggcgtcg ctcccgtagt gaaggcccag catgatcagg gcgtcgccga
      421 gacccgtgaa gccgaggccg atgcggcggc tggcgcgcgc ctgctccgcc tgctgcggca
      481 gcggatagcc ggaaacgtct atgaccgaat cgagaaagcg aacggcctcg ggcactcggc
      541 ggcgcagggc gtcgaagtcg aaggcggcgc ccgcggcgaa cgggtctcgc acgaacgacg
      601 tgaggttgaa gctgccgaga tcgcaggcgc cgtaggcggg cagagggacc tcgccgcagg
      661 gattggtggc cgagaggcgc tcgcagtagc tgagattgtt ctcgtggttg atgcggtcga
      721 tgaacagcac ccccggctcg gagcagtcgt acgtcgcccg catgatctgt tcccacagct
      781 cgcgcgcccg gaccctgcgc accacggcgc acgggacggc tccatcttcc ccgggccatt
      841 cgcgccggat gatgtcggtg ccctggtccg tcggcaggtg gtgggcggga aagaccaggg
      901 gccattcggc gtcgtcgcgc aacgcgttca tgaacgcatc ggtaacgagg accgagcagt
      961 tgaaatgggt cagggcgtcg gggtcccgtt tggcatcgac gaaggcatgg atgtcgggat
     1021 gatcgcagcg aagggtcgcc atcatggcgc cgcggcgggc tccggtagag cacagggtgg
     1081 cacacatggt gtcccagatg tgcatgaacg agaccggccc ggaggccgtg ttgccggttt
     1141 cccgggcggg cgttcccacc gggcgcaggg tggagaagtc gtagccgacc ccgccgcccg
     1201 cctgcatggt cagcgcgcct tccttcagag cggtgaagat gggctccatc gcgtcttcga
     1261 tggtgcccat gacgaagcag ttgcaaaggg tgacccggcg cccggttcca gcgccagcca
     1321 ggatccggcc cccgggaagg aagcagaaat cacccagcag atcccggtaa gcggaagccc
     1381 aacggcccgg ctcccgctcc cgggaagcga tcgtgtccgc gacacgctgc cacgtatcct
     1441 cgatggtttg atcgaccggc tcgccgcttc gatagaagcg gtagcgggcc tcccagacct
     1501 cccgcgcgat gtcggtccgc aaagggtccg agatggctac gactcgactt cgacccgacg
     1561 gcgcttggag tcctccgcct ttggaatcgt gacctccagc atgccgtcgc gcagcgtcgc
     1621 cttggcatcg tcgccccgaa cccgcgccgg cagcgaaacg gtgcgggcga cgtagctcgt
     1681 ggagatctcg cggcgatgat agtcaccctc ctcggtctcc tcttccttgt gtgaggagcc
     1741 cttgatgctg aggctgtcat cgctgacgga cacctcgatg tcgtctttgc tcattcccgg
     1801 cagctcggca cggacgaaga tctcgtgctc gcggtccacc acgtccacct tcgggccctt
     1861 cttttccaga atcgattcga atccaggcca ctcgcggctc cacggccgca gccagcgctt
     1921 gtcgaagaaa tcctcgaaga gccgttccat gtcgcgctcg cgcggtgccg ttgccaaccc
     1981 tttactttcc tcggcaggtt ccgcctgctc ggcccttatt gccggcttgg cgctttccac
     2041 ggaaatctga gactcgctca tttcacatct cctcaatcag tccacggggg attatctgcc
     2101 cgctgtggaa ggcacgtgta tccgaacaaa tacttacccg gatccggttt tctcgaggga
     2161 accgggcggc gattcggcgc ggccggccga ccgcgccgtc taggtgaaat acctgatgac
     2221 gcgggcccgg caggcatgtc acattgcccg gagttgcaac gggtgaagcc ttaccgtgga
     2281 aacggcaagc cgtgaagacg tgaacctctg ggtggccggc tctttgaagg agatggccag
     2341 cctactggag gatcaggccg ccaacccgtt ccgcatcaat gcctaccggc gggcggcgcg
     2401 gaccttggag ggcctggacg agccggtgga tgcgctctac cggcgctcgg ggcgcgaggg
     2461 gttgatggcg ctgccttcca tcggcgaagg catcgcgacc gccatcgaga acctgctgcg
     2521 caccggcagg ctgccccagc tcgagcggct gcgcggcagc gtggagccgg agacggtgtt
     2581 cgactccctg cccggcgtcg gcccgcgcct ggcggcagcc attcacgaca ccctgcacgt
     2641 ggacaccctg gaggagctgg aagtggccgc ccacgacggt cgtctgcaga cggttcccgg
     2701 catcggcccc agccgggcgg ccagtatcag agcgctgctt gcagagcggc tgaagcggcc
     2761 gccgcgtcgc gccctggcaa gggcgccgag tgtcgccgcc atactggatg ccgacgggca
     2821 atatcgacgc gaagccgcag ccggccggct gcgcaccatc gccccgcgcc gtttcaatcc
     2881 gtcccacaag gcctggctcc ccgtgctgca caccgaccgc aacgactggc acatgacgct
     2941 gctcttttcc aacaccgcca gggcccacga gctcggcaag acggatgact gggtggtcgt
     3001 ctactactac gacggcgatc acaacgaagg ccagtgcacg gtggtcaccg agacggcggg
     3061 gccgcgcagg ggcgcgcgcg tcgtgcgcgg gcgtgagaac gaagcctagc ccatggcgtc
     3121 gaacccgacg ccttttcggg ctcccgattg agcgctagcc gatcggcacc cagatcccga
     3181 gatcgcagcg cagcttcatc gggccgctct ttaccacggc gccgtttgga aatgcttcgg
     3241 aattgaagta gcagaagggc tcgtccggga tctcttctct gag
//
LOCUS       DBNHQR010000570         2057 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1069177, whole genome shotgun sequence.
ACCESSION   DBNHQR010000570 DBNHQR010000000
VERSION     DBNHQR010000570.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1069177"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="AC16VR_13010"
     CDS             <1..647
                     /locus_tag="AC16VR_13010"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003222172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HIR6012123.1"
                     /translation="GIGMTSQRTRDRLVQRLAEHGIVDQEILDLIGHVPRHIFVDEAL
                     SHRAYEDSALPIGHGQTISQPFVVAFMTSTLLNAPRARVLEVGTGSGYQTAVLRSSRR
                     VRRVFTVERLGPLLARAEERLKAMGLESVRYRHGDGYEGWPEEAPFDGILVTAAPRQV
                     PEALLDQLAEGGRMVVPVGGGGVQELRVYDRTATGVNVDTVRHVRFVPLVKGLL"
     gene            658..1530
                     /locus_tag="AC16VR_13015"
     CDS             658..1530
                     /locus_tag="AC16VR_13015"
                     /inference="COORDINATES: protein motif:HMM:NF015951.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HIR6012124.1"
                     /translation="MSARGARQWSGLFARGMAMGVAEVVPGVSGGTIAFITGIYHELV
                     TTLAGFRPASARLLVTAGPVAFWQQHNLGFLAVLVAGMAASVLLFARLIGYWLDVVPT
                     LVWAFFFGLIAASVFQIGRGRPTRTLAVYGTIGLAAGASLLLVDPLPAQGAVWVFFFA
                     GMIAISAWLLPAISGSFILLILGLYEPVIEALNGFDWVVLTSLGLGCATGLMIFARAL
                     EWLLSYWREPLLSLLTGFMAGSLVRLWPWAQGGTLLTPAEYAAVTGQASLELWAVAAA
                     AAGALALWLLSRLE"
     gene            complement(1547..1870)
                     /gene="fdxA"
                     /locus_tag="AC16VR_13020"
     CDS             complement(1547..1870)
                     /gene="fdxA"
                     /locus_tag="AC16VR_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HIR6012125.1"
                     /translation="MTFVIGENCIKCKLTDCVEVCPVDCFYEGPNMLVIHPDECIDCA
                     LCEPECPVDAIYSEDELPEDQQDFLEINREQAELWPNITEKKDPAEDYEDWIGKPDKR
                     HLLER"
BASE COUNT          285 a          647 c          689 g          436 t
ORIGIN      
        1 tcggcatcgg catgacctct cagcgcaccc gggatcggct cgtgcagcga ctggccgagc
       61 acggcatcgt cgatcaggaa atactcgacc tgatcggcca cgtgccccgg cacatcttcg
      121 tggacgaagc gctgtcccac cgcgcgtacg aggattcggc cctgcccatc ggccacgggc
      181 agaccatctc gcagcccttc gtcgtcgcct tcatgacctc cacgctgctg aacgcgccac
      241 gtgcgcgggt tctggaagta gggaccgggt ccggatacca gacggccgtg ctgcgatcga
      301 gccggcgcgt tcgccgggtc ttcaccgtcg agcgtctcgg gccgctgctc gcccgcgccg
      361 aagagcgctt gaaggccatg gggctggaat cggtcaggta ccgccatggc gacggttacg
      421 aaggatggcc ggaggaagca ccgttcgacg gcattctcgt gacggcagca ccgcggcagg
      481 ttccggaagc tctgctggat cagctggcgg aaggcgggag gatggtggtg cccgtgggcg
      541 gcggtggcgt gcaggaactg cgcgtttacg atcgcactgc caccggcgtc aacgtggaca
      601 cggtgaggca cgtacgcttc gtgccgctgg tcaagggcct gctatgaggg tcgcagcgtg
      661 agcgcccgcg gcgcccgtca gtggagcggt ctcttcgcgc gcggcatggc catgggcgtc
      721 gccgaggtgg ttcccggtgt gtccggaggc accattgcct tcatcacggg catctaccac
      781 gagctcgtga ccaccctggc cgggttcagg cccgcttccg cgcgtctgct cgtgaccgca
      841 ggaccggttg cgttctggca gcagcacaat ctggggtttc tggccgtgct ggtggcgggt
      901 atggctgcca gcgttctgct cttcgcccgg ctcatcggct actggctcga cgtggtgccg
      961 accctggtct gggctttctt cttcgggctc atcgccgctt cggtgttcca gatcggccgc
     1021 ggtcgcccga cgcgtacgct tgccgtgtac ggcaccatcg ggctcgctgc cggggcatcg
     1081 ctcctgctcg tcgacccgtt gcccgcccaa ggggccgttt gggtcttctt ctttgccggg
     1141 atgatcgcca tctctgcatg gctgctcccc gctatctcgg gtagcttcat cctgctgatt
     1201 ctcgggctct atgagcccgt catcgaggcg ctgaacgggt tcgactgggt ggtgctcacg
     1261 agtctgggtc tcggatgtgc caccgggctg atgatcttcg cccgggcgct ggagtggctg
     1321 ctctcctact ggcgcgagcc gttgctttct ctactgacgg gattcatggc gggctcgctg
     1381 gtgcgcctgt ggccctgggc ccaaggcggc accctgctga cgccggccga gtatgctgcg
     1441 gtgacgggtc aggcgagtct ggaactgtgg gcggttgctg cggccgctgc gggcgcattg
     1501 gccctctggc tgttgtccag gctggagtag acgcaggccg aatcggtcag cgttcgagga
     1561 gatggcgctt gtcgggtttg ccgatccagt cctcgtagtc ttccgcagga tccttcttct
     1621 cggtgatgtt cggccacagc tcggcctgct cgcggttgat ctccaggaag tcctgctgat
     1681 cctcgggaag ctcgtcttcc gagtaaatgg catccaccgg gcattcgggc tcgcaaaggg
     1741 cgcagtcgat gcattcgtcc ggatggatga cgagcatgtt tggcccttcg tagaagcagt
     1801 ctaccgggca tacctcaacg cagtctgtga gcttgcactt gatgcagttt tccccaatga
     1861 cgaacgtcat ctgtattgcc ttcctgtcgg gtaagttggt gtttcgggtc tgcgaaggga
     1921 ccgtattcta accatattcg atcccctgtc gagcgggcgc ccgtcagcta tcgggggacg
     1981 ccaaaccctt gagttcgtag agcagcgcga gggcttcccg agggctcaac ccgtctacgt
     2041 ccagctcgga cagcctc
//
LOCUS       DBNHQR010000571         6133 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_753137, whole genome shotgun sequence.
ACCESSION   DBNHQR010000571 DBNHQR010000000
VERSION     DBNHQR010000571.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6133)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6133)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6133
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_753137"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..1723
                     /locus_tag="AC16VR_13025"
     CDS             185..1723
                     /locus_tag="AC16VR_13025"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR6012126.1"
                     /translation="MEVRTLMARAAAHYADREAVIHGDRRLTFREAWQRSLRLGNGLR
                     GLGLTPGDRIGVLEDNSVEAADLFGGAAVANLVRVPLYPRNGRDAHRHMLGHTNCRAL
                     IVSENHAEEVAGLADELPDLEHVIIRDAGYDGWLAAQTDEDPRLPISSDDYFIIRHTG
                     GTTGLSKGVAYTHKAWLAAGRDWFYAFPPVEPGDKCLHLGPISHGSGYQYLPIWLAGG
                     CNVMMDHFDTGETLDLIEREGIAYAFMVPTMVNAVVHDNSARGRDFSSLKCLLIAAAP
                     IQDATALAAREVFGDVLWQGYGQTEVVPVSMMGPRQWFAEVEGSSPLRACGLPLPFAE
                     VQIWDEDNQPVAAGEVGEIVARADGQMTGFWNNPEASAERIVDGWVKTGDLGRLDANG
                     YLYVVDRADDMIISGGYNIWPAELENVLASHPAVIEVAAFGVPSQKWGESPYAVCVVG
                     NAEAVGEAELIDLVAEELGSYKKPVGVTITTDPLPKSPVGKIKRKDLREPFWKGHERR
                     VAGS"
     gene            complement(1742..2158)
                     /gene="arfB"
                     /locus_tag="AC16VR_13030"
     CDS             complement(1742..2158)
                     /gene="arfB"
                     /locus_tag="AC16VR_13030"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HIR6012127.1"
                     /translation="MSVSRIRQIPPEACSERFVRSPGAGGQNVNKVATTVQLRVDLDR
                     ANLPPAVRERLEKLAGRQVNQRGELVITASRFRTQARNRADAMSRLSELLEQAQRVPR
                     RRVATRTPAGERVRRKDTKRKRGTTKRLRARPPMDD"
     gene            complement(2207..3343)
                     /gene="epmB"
                     /locus_tag="AC16VR_13035"
     CDS             complement(2207..3343)
                     /gene="epmB"
                     /locus_tag="AC16VR_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008174110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HIR6012128.1"
                     /translation="MSRRPTRDRDRDRLRYETADDCAILVPDVNPMTQSAPEQSAARL
                     RTRTVADDPATGSWQEALRNAIRTPRELIEALELDPAWAPVMDGHDDDFPMLVPRSFA
                     ARMRKGDPHDPLLRQVLPDGAERITAPGFEADPLQEQRFARAGVVRKYSGRALLIASG
                     ACPVHCRYCFRRNFPYADQLASRQRWTGAVDALSADTQVTEVILSGGDPLTLTNEAIG
                     ELIRAMENVASVQTLRFHTRFPIIIPERVDAGLVDTLAATRLRTVFVVHCNHANEIDA
                     SVRRALADLKAAGAQLLNQSVLLKGVNDNEDDLAELSQRLFDAGVLPYYLHLLDPVAG
                     AAHFDVAPARGRALVAGLRRRLPGYLVPRLAREVPGESSKTILA"
     gene            complement(3340..4644)
                     /locus_tag="AC16VR_13040"
     CDS             complement(3340..4644)
                     /locus_tag="AC16VR_13040"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR6012129.1"
                     /translation="MKFSARTATAVIMANMIGTGVFTSLGFQLADIRSEFVLLMLWAV
                     GGLTALCGALSYAELGAALPRSGGEYNFLGRIYHPAVGFVSGWISATIGFAAPTALAA
                     ITFGIYLASVFPALSPVWLACGLVVVLSVAHATSRRSSGGVQRAFTAVKIILILGFCL
                     LAAVLVDEPQGISLLPHEGDGALLTSGAFAVALIYVNYAYTGWNAATYLTGELDRPEQ
                     TLSRVLLQGTVVVMLLYVLLNATFLYVAPAEAMEGRLEIGYIAAEHAFGSNGAAIMAV
                     ILALLLISTASAMIMAGPRVLQVIGQDYPAFRFLARVSGDGVPVTAIVVQSVLALAFI
                     LTASFEAILVFAGFTLGVSTFFTVLGLFVLRIRQPDLPRPYRTTWYPLPPLVYLAITG
                     WTLGYIIAHRPKEGLWGLAIIAGGALLYLLIRRGRPTEEVSS"
     gene            complement(4688..5128)
                     /locus_tag="AC16VR_13045"
     CDS             complement(4688..5128)
                     /locus_tag="AC16VR_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012130.1"
                     /translation="MARLALTGAIIILLSGCAEPFIVFAGGALSGDEKVPPADWSFLE
                     AIDTVQIETDPNDPYSVNVWSVGIGADVYVATGEDGTNWTERIDGDPSVRLRTNGDIY
                     PLRATAVTEMAERRRVAQAYLNKYDVDDDEGWVDTGKIYRLDRR"
     gene            5258..>6133
                     /locus_tag="AC16VR_13050"
     CDS             5258..>6133
                     /locus_tag="AC16VR_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017338569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HIR6012131.1"
                     /translation="MRAMQLTEPGGDFRLVDLPKPRPAAGEVLLAVHACGVCRTDLHV
                     VDGDIVDGRYPVVPGHQIVGVVEQAPDGDLQPGQRVGVPWLGHTCGTCVFCLAGQENL
                     CDSAEFTGYHRQGGFAEYAVADARFCFPIPESYPDVQAAPLLCAGLIGYRALGMVGDA
                     RKLGFYGFGNAAHILTQIAVWEGREVSAFTRPGDAAGQRFARGLGAVWAGDASTPPPE
                     PLDAAIIFAPDGALVPAALKHVRKGGVVVCAGIHMSDIPSFPYADLWGERIIRSVANL
                     TREDGVRLLEVAPRVP"
BASE COUNT         1013 a         1969 c         2127 g         1024 t
ORIGIN      
        1 aacaatgacg tgaagctcat ctgttgatcc caatgctcgg aagtgtccct gcggacgatg
       61 aaatacccga cattgccatg ccgatcaagg gtggtaggct tttgcgcttg tcctgccgcc
      121 ggtctgagcg gcgcgctggt cggtcgccct gctgaggcga tcgggcccgg aaggagggga
      181 gatcatggag gtccgtacgt tgatggctcg ggcggccgcg cactacgccg atcgcgaggc
      241 ggtcattcac ggcgaccgtc ggctgacctt ccgcgaagcc tggcagcgtt ccctgcggct
      301 cggcaacggc cttcgtggcc tggggctcac cccgggggat cgcatcggcg ttttagagga
      361 caactcggtg gaggcggccg acctcttcgg cggcgctgcg gtggcgaacc tagtgcgcgt
      421 gcccctttac ccgcgcaacg gccgcgacgc ccatcggcac atgctcggcc acaccaactg
      481 ccgcgcgctg atcgtcagcg agaatcacgc ggaggaggtg gccgggctcg cagacgagct
      541 gcccgacctc gagcacgtca tcatccgcga cgcgggctac gacggctggc tcgcggccca
      601 gaccgacgag gacccgcgtc tgcccatctc ctcggacgac tacttcatca ttcgccatac
      661 cggcggcacg acgggcctct ccaagggagt ggcctacacc cacaaggcct ggctcgccgc
      721 ggggcgcgac tggttttacg cgtttccccc ggtggagccg ggcgacaagt gcctgcatct
      781 gggccccatc tcccacggct cgggctatca gtacctgccc atctggctcg ccggcggctg
      841 caacgtgatg atggaccact tcgacaccgg ggaaaccctg gatctaatag agcgcgaagg
      901 aatcgcgtac gcgttcatgg tgccgaccat ggtgaacgcc gtcgtccatg acaactcggc
      961 cagggggcgc gatttcagca gcctgaaatg cctgctcatc gcggccgcgc cgatccagga
     1021 cgcgaccgcg ctggcggccc gtgaggtctt cggtgatgtg ctctggcagg gctacggtca
     1081 gacggaggtg gtgccggtgt ccatgatggg cccgcgccag tggttcgccg aggtggaggg
     1141 ctccagccct ctgcgggcat gcggcctgcc gctgccgttt gcagaggtgc agatctggga
     1201 cgaggacaac cagccggtgg ctgcgggcga ggtgggcgag atcgtggccc gcgcggacgg
     1261 gcagatgacg ggtttctgga acaatcccga ggcgagtgcc gaacgtatcg tggatggctg
     1321 ggtcaagacg ggcgatctcg gcaggctgga tgccaatggc tatctgtacg tggtggaccg
     1381 ggcggacgac atgatcatct cggggggcta caacatctgg ccggcggagc tcgagaacgt
     1441 tctcgccagc catccggccg tcatcgaggt ggcggcattc ggcgtgccca gccagaaatg
     1501 gggcgagtcg ccgtatgccg tttgcgtggt cgggaatgcc gaggcggtcg gcgaggccga
     1561 gctcatcgat ctggttgccg aggagctagg cagctacaag aaacccgtcg gcgtgaccat
     1621 caccacggat cccctgccaa agagcccggt cgggaagatc aagcgcaagg atcttcgcga
     1681 gcccttctgg aagggacacg agcggcgcgt cgccggcagc tgaccggttc aggcctggcc
     1741 ctcagtcatc catgggcggc ctggcccgga ggcgcttggt ggtcccccgc ttgcgcttgg
     1801 tgtccttgcg tcgtacccgc tccccggctg gcgttctggt ggcaacccgg cgtcggggca
     1861 cgcgctgcgc ctgttccagc agctcgctca accggctcat cgcgtcggcc cggttgcgcg
     1921 cctgggtccg gaagcggctg gccgtgatga cgagctcccc gcgctgattc acctggcgcc
     1981 cggccagctt ctccagtcgt tcgcggacgg ccggcgggag gttggcgcga tcgagatcga
     2041 ctcggagctg caccgtggtg gcgaccttgt tgacgttctg cccacccgcg cccgggctcc
     2101 ggacgaaacg ctcggagcaa gcctcgggcg ggatctgcct gatacgtgaa acgctcattg
     2161 gccaatcata gcagcggcgc cttctttcca gacgcgggcc ggcacgctag gcgaggatgg
     2221 tcttgctcga ttcgccgggc acctcgcgtg ccagcctggg caccagatag ccgggcagcc
     2281 tgcgacgcag accggccacc agggcacgcc cgcgcgctgg cgccacgtcg aagtgggcgg
     2341 cgccggccac cggatccaga aggtgcaggt agtagggcag cacgccggca tcgaacagcc
     2401 gttgactcag ctcggccagg tcatcctcgt tgtcgttgac tcccttgagg agcacggact
     2461 ggttcagaag ctgggcgccc gcggccttga ggtccgccaa cgcccgtcgc accgaggcgt
     2521 cgatctcgtt ggcatgattg cagtgcacga cgaaaaccgt ccgcaagcgg gttgcggcca
     2581 gcgtatcgac caggcccgcg tcgacccgtt cgggaatgat gatggggaag cgggtgtgga
     2641 accgcagggt ttggacgctt gcgacgttct ccatggcgcg aatcaactcg ccgatcgcct
     2701 cgttggtcag cgtgagtgga tcgccgcctg aaagtatgac ttccgtcacc tgcgtgtccg
     2761 cgctcagcgc gtcgacggcg cccgtccagc gttggcgtga cgcaagctgg tccgcgtagg
     2821 ggaaattcct gcggaagcag tagcggcagt ggacgggaca cgcgccggag gcgatcagca
     2881 gcgcgcggcc ggaatacttt cgcacgacgc cggctcgggc aaagcgctgc tcctgcagcg
     2941 ggtcggcttc gaagcccggc gccgtgatgc gctctgcccc atcggggaga acctggcgca
     3001 gcagggggtc gtggggatct cccttgcgca tgcgtgccgc aaaggatctc ggaaccagca
     3061 tgggaaagtc atcgtcgtga ccgtccatca ccggcgccca ggccgggtcg agctccaggg
     3121 cctcgatcag ctcccggggc gttctgatgg cgttgcgcaa cgcctcctgc cagctgccgg
     3181 tcgccggatc gtcggcgacc gtgcgtgtcc gcagacgggc tgcggattgc tcgggtgcac
     3241 tttgagtcat ggggttcacg tccgggacga gtatcgcgca atcgtctgcg gtctcatagc
     3301 gcagccggtc ccggtcccgg tcccgggtcg gccgtcgact caagaagaga cctcctccgt
     3361 cggccgcccc cgccggatca ggaggtagag gagcgcgcct cctgcgatga tggccaggcc
     3421 ccacaacccc tctttcggtc ggtgggcgat gatgtagccg agggtccatc ccgtgatggc
     3481 caggtagacg agcggcggta gcgggtacca cgtcgtgcga taggggcggg gcaggtcggg
     3541 ctggcggatg cgcagcacga acaggcccag caccgtgaag aacgtgctga ccccgagggt
     3601 gaagccggca aacacgagaa tggcttcgaa agacgctgtg agaatgaagg ccagcgccag
     3661 cacggactga acgacgatgg ccgtcaccgg taccccgtcg ccgctcaccc gagccagaaa
     3721 acgaaacgcg gggtagtcct ggccgatcac ctgcaaaacc cttggacccg ccatgatcat
     3781 ggcgctggcg gtggatatca gaagcagagc cagaatgacg gccatgatgg ccgcgccgtt
     3841 cgagccgaag gcgtgctcgg cggcgatgta gccgatctcc agcctgccct ccatggcctc
     3901 cgccggcgcg acgtacagaa aggttgcatt caaaagcacg tacagcagca ttacgaccac
     3961 ggttccctgg agcagcaccc gggagagtgt ctgctcgggg cggtccagct cgcccgtgag
     4021 gtaggtggcc gcgttccagc ctgtgtaggc gtagttgacg tagatcaaag ccacggcgaa
     4081 cgcgccgctg gtgagcagcg ctccgtcacc ctcgtggggc agcagcgata tcccctgagg
     4141 ctcgtcgacc agcacggcgg cgagcaggca gaaaccgaga atcaggatga tcttcacggc
     4201 agtgaacgcc cgctggacgc cgccgctcga gcgtcgcgag gtggcgtgag caacggaaag
     4261 caccacgacc agaccgcacg ccagccagac cggcgacagc gcgggaaaga ccgaggccag
     4321 atagatgccg aaggtaatgg cggcgagcgc cgtgggtgcg gcaaacccga tggtggcgga
     4381 gatccagccg gacacgaagc ctacggccgg atgatagatt cgtcccagga agttgtactc
     4441 gccacccgag cgcggcagag ccgcgccgag ctccgcgtag gacagcgccc cgcagagcgc
     4501 cgtcaacccc cccaccgccc agagcatcag caggacgaac tcggatctga tgtcggccag
     4561 ctgaaagccc aggctcgtga acacgccggt accgatcatg ttggccatga tcacggccgt
     4621 ggctgtgcgc gcgctgaatt tcatgagccc ggggcctcag gcacccgtca cggtgagccg
     4681 gtcgcgacta gcgccggtcc agacgataga tcttgccggt atcgacccag ccttcgtcat
     4741 cgtcgacgtc gtacttgttc aggtaggcct gagcgacgcg cctgcgctcg gccatctcgg
     4801 tgacggcagt cgcgcgcagc gggtagatgt cgccgttggt cctgagcctt acgctcgggt
     4861 caccgtcgat tcgctccgtc cagttggtgc cgtcctcgcc cgtggccacg tacacgtctg
     4921 cgccgatccc gacggaccac acgttcacgg agtaggggtc gttcggatcc gtctctatct
     4981 gcacggtatc gatggcttcc agaaaggacc agtcggccgg cggcaccttc tcgtcgccgc
     5041 tgagcgcccc cccggcgaag acgatgaacg gctctgcaca gccgctcagg aggatgatga
     5101 ttgcgcctgt gagtgcgagt cgcgccatgg cctgaccttc gagaagacgg caattagggc
     5161 gaattctttg ccgcctgtaa atagatttct cgccgtgatc ggcccacgga ccgtatgatc
     5221 tgagggtccg gtcgcttgcg tatgggaggg cgtggacgtg cgcgcgatgc agcttacgga
     5281 gcccggtgga gatttccgcc tggtggactt gccgaagcct cgaccagcgg cgggcgaggt
     5341 tctactggcc gtgcacgcct gcggcgtctg ccgcaccgat ctccacgttg tggacgggga
     5401 catcgtcgac ggccgctatc ccgtcgtgcc cgggcaccag atcgtcgggg tggtcgagca
     5461 ggcgccggac ggcgatctgc aaccgggaca gcgggtgggc gtcccgtggc tcggccatac
     5521 ctgcggcacc tgcgttttct gccttgcggg ccaggagaac ctgtgcgatt cggccgaatt
     5581 caccggatac catcgccagg gtggattcgc ggagtacgcg gtcgccgacg cccgcttctg
     5641 tttccccatc ccggaaagct atccggacgt tcaggcggcg ccgctgctgt gcgcggggct
     5701 gatcggctac cgggccctgg gaatggttgg ggatgctcga aagctgggtt tctacgggtt
     5761 cgggaatgcc gcccacatcc tcacccagat tgcggtatgg gaggggcgcg aagtgtccgc
     5821 gtttacccgc ccgggggatg cggccggcca gcgattcgcg aggggtctgg gtgctgtgtg
     5881 ggcgggtgat gcgtcgacgc cccctccgga gcccctggac gccgccatca tcttcgcgcc
     5941 ggacggggcg ctggtgccgg cggccttgaa gcatgtgcgc aagggtgggg tggtggtctg
     6001 cgcgggcatt cacatgtccg acatcccttc gttcccctac gccgatctct ggggcgagcg
     6061 catcatccgc tcggtggcca acctgacccg cgaagacggc gtgcggttgc tggaggtggc
     6121 gccccgcgtc ccg
//
LOCUS       DBNHQR010000572         3322 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1576500, whole genome shotgun sequence.
ACCESSION   DBNHQR010000572 DBNHQR010000000
VERSION     DBNHQR010000572.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1576500"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /gene="cysS"
                     /locus_tag="AC16VR_13055"
     CDS             complement(<1..1052)
                     /gene="cysS"
                     /locus_tag="AC16VR_13055"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HIR6012132.1"
                     /translation="MDIRLHNTKSGRKEAFDPVDPQRITMYVCGPTVYGLVHIGNGRP
                     AVVFDVLYRLLRAAHPEVVYARNITDIDDKINAAARDNDEPIQALADRFSSAYREDVA
                     ALGVLEPTVEPRATHHIAEIIAMIETLIAKGNAYEAQGHVLFHVPSDPEYGSLSHRSL
                     EDMLDGARVEVAPYKQDPKDFVLWKPSTTDLPGWDSPWGRGRPGWHIECSAMISKHLG
                     QTIDIHGGGSDLAFPHHENEAAQSRCANDHPEYVRYWLHNGMLTMGQEKMSKSVGNIV
                     TIRDLLDRFPGEVLRYALLSGQYRSQLAWSEDLLDQAGSSLERLYQALRDAGVEDETS
                     SDYRDLPAEEYPTVVVA"
     gene            complement(1071..1472)
                     /gene="hisI"
                     /locus_tag="AC16VR_13060"
     CDS             complement(1071..1472)
                     /gene="hisI"
                     /locus_tag="AC16VR_13060"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015637550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HIR6012133.1"
                     /translation="MFEYLEDAELGHEVALDALLDAVKFDSDGLVPVIAQDADSRDVL
                     MLAWMNREAVERTLKDGYACYWSRSRGAFWRKGESSGHLQALKEMRFDCDGDAILLIV
                     DQTGPACHTNRPSCFYLSVSDGTARVSSEPV"
     gene            complement(1474..2838)
                     /locus_tag="AC16VR_13065"
     CDS             complement(1474..2838)
                     /locus_tag="AC16VR_13065"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013079.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HIR6012134.1"
                     /translation="MAEALIVSSDAAATPLRLIRTSDFEAFASELSEAERSWIRRAQF
                     AAKSGEFAWLPGADGHPAELVAGWDGGDDLASLGGLPTRIPEGVYELKTEVSDLQLLG
                     WALGSYQFVRYRERDRAPAQLLIPARNDADLVANMDAATTLVRDLINIPAGDMLPSHL
                     ADEAIQVADANQANCRVLVGEQLLEGGFDAIHAVGRAAADAPRLIDITWGDPDHPLIV
                     LVGKGVCFDSGGLDIKPAASMRLMKKDMGGAANALGLAQLIMASNLPVRLRVLIAAVE
                     NAVGANAYRPGDVVPTYKGISVEIDNTDAEGRMVMCDCLALGAEDEPALIVDFATLTG
                     SARSAVGAEIAAMFCNDDEVAAGLSAHGDRWDDPVWRLPLHKPYAYLLDSKVADTVNS
                     ASSPFAGSITAALFLEKFVGSAPWVHFDIMAFNTRPRPGRPEGAEAMAIRAVFGYLQE
                     RFGG"
     gene            2907..>3322
                     /locus_tag="AC16VR_13070"
     CDS             2907..>3322
                     /locus_tag="AC16VR_13070"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6012135.1"
                     /translation="MLTEVTTHHLELTDPDQFKPAATGPAYALKQVQRPTAELNRFLY
                     STVGAAWFWYERLSWSRARWLEFLDRPEVVTWVAYVDGSPAGYFELEDQPGGSVEICY
                     FGLLPAYIGQGMGGALLTDAIRAAWARGASRVWLHT"
BASE COUNT          509 a         1080 c         1126 g          607 t
ORIGIN      
        1 gccaccacga cggtcgggta ctcttcggcg ggcaggtcgc ggtagtcgct ggatgtctcg
       61 tcctccacgc cggcatcacg cagcgcctga tacagacgct ccaggctgct acccgcctgg
      121 tcgaggagat cctccgacca ggccagctgg gaacggtact gacccgaaag cagcgcgtag
      181 cgcaggactt cccctggaaa gcgatccaga aggtcgcgga tggtgacaat gttccccacg
      241 gacttggaca tcttctcctg gcccatggtg agcatgccgt tgtgcagcca gtagcggacg
      301 tactcgggat ggtcgttcgc gcagcggctc tgagcagctt cgttctcgtg gtgaggaaac
      361 gccagatccg agccgccgcc gtggatgtcg atggtctggc cgagatgctt gctgatcatc
      421 gccgagcact cgatgtgcca gccgggccgc ccccgtcccc aggggctgtc ccagcccgga
      481 agatccgtcg tggaaggctt ccacagcacg aaatccttgg ggtcctgctt gtacggcgct
      541 acctctaccc gcgcgccgtc gagcatgtcc tcgaggctgc ggtgggaaag gctgccgtat
      601 tcgggatcgg agggaacgtg gaagagaacg tgcccctgag cctcgtaggc gttgcccttg
      661 gcgattaggg tctcgatcat cgcgatgatc tctgcgatgt ggtgtgttgc cctcggttcc
      721 accgtgggct cgagcacgcc cagcgcggcc acgtcttccc ggtaggcgct gctgaaacgg
      781 tcggccaggg cctggatggg ctcgtcgttg tcccgggcgg cggcgttgat cttgtcgtcg
      841 atgtccgtga tgttgcgcgc gtagaccacc tcggggtgcg cggcgcggag cagtcggtag
      901 agcacgtcga acaccacggc cgggcgcccg ttgccgatgt gcaccaggcc gtagacggta
      961 ggcccgcaga cgtacatcgt tatgcgctgg ggatcgacgg gatcgaaggc ttccttgcgg
     1021 ccggatttcg tgttgtggag tcggatgtcc ataagcggct cgccgtctgc tcagaccggt
     1081 tcggacgaca cgcgcgcggt gccgtcgctg acgctaaggt aaaagcacga gggccggttc
     1141 gtgtggcagg ccggccccgt ctggtccacg atgagcagga tggcgtcgcc atcgcagtcg
     1201 aagcgcatct ccttgagcgc ctgcagatgt ccggaactct cgcccttgcg ccagaaggcc
     1261 ccgcggctgc gagaccagta gcaggcgtag ccgtctttca gggtgcgttc gaccgcctcg
     1321 cggttcatcc aggcgagcat gagaacgtcc ctgctgtcgg cgtcctgggc gatgacgggc
     1381 accaggccgt ccgagtcgaa cttgaccgcg tccagcagcg cgtcgagggc gacctcgtgg
     1441 ccgagctccg cgtcctcgag gtattcgaac atctcagcct ccgaatcgtt cctgcaggta
     1501 gccgaaaacg gccctgatcg ccatggcttc cgcaccctcg ggtcggcccg ggcgggggcg
     1561 tgtgttgaag gccatgatgt cgaagtgcac ccacggcgcc gagcccacga acttctccag
     1621 gaagagggcg gccgttatgg atccggcgaa cggcgagctt gccgagttga cggtgtcggc
     1681 caccttgctg tcgagcaggt aggcgtaggg tttgtgcagc ggcagccgcc acacagggtc
     1741 gtcccagcgg tcgccgtggg cgctcagccc ggcggcgacc tcgtcatcgt tgcagaacat
     1801 cgccgcgatc tctgcgccca cggccgatcg cgcggatccg gtcagcgtgg cgaaatcgac
     1861 gatgagggcg ggttcgtcct cggcacccag cgccaggcag tcacacatga ccatgcgccc
     1921 ttcggcgtcg gtattgtcga tttccaccga gatgcccttg taggtcggca cgacgtcgcc
     1981 aggccggtaa gcgttcgcgc cgacggcgtt ctccactgcc gcaatgagca cgcgcagacg
     2041 aacgggcaga ttgctcgcca tgatgagctg ggcgaggccg agagcgttgg ctgccccgcc
     2101 catgtccttt ttcatgagcc gcatactggc ggccggcttg atgtcgagcc cgccgctgtc
     2161 gaagcacacc cccttgccca ccagcacgat cagcggatgg tccgggtctc cccaggtgat
     2221 gtcgatcagg cgcggcgcgt cggccgccgc ccgcccaacg gcgtgaatcg cgtcgaagcc
     2281 gccctccagc agctgctcgc ccaccagcac ccggcagttt gcctggttgg cgtccgccac
     2341 ctggatggcc tcgtctgcga gatggctcgg cagcatgtcg cctgcgggga tgtttatgag
     2401 atcgcgcacc agggtagtgg ccgcgtccat gttcgcaacg aggtccgcgt cgttgcgtgc
     2461 aggaatcagg agctgggcgg gagctctgtc gcgctcccgg tagcggacga actgatagga
     2521 accgagcgcc cagccgagca gctgcaggtc ggagacctcg gttttcagct cgtacacgcc
     2581 ttcgggtatg cgggtgggaa ggccgcccag gctggccagg tcgtccccgc cgtcccatcc
     2641 tgccaccagc tcggccgggt ggccgtcggc tccgggcagc caggcgaact cgccgctctt
     2701 cgccgcgaac tgagcacgcc ggatccagct ccgttcggct tccgaaagct cgctggcaaa
     2761 cgcctcgaag tccgaggttc tgatcagcct gagcggcgtt gcggcggcgt cggatgaaac
     2821 gatgagcgct tctgccattg gggctccggt tggttgaagt ccgagatggt ctatgatggc
     2881 ctcccggttc gcaagcggtc tgagccgtgc tcacggaagt caccacgcat cacctcgagc
     2941 tcaccgatcc ggatcagttc aagccggcgg ccaccggtcc ggcctacgcg ctcaagcagg
     3001 tccagcgtcc caccgcggag ctgaaccgct ttctctactc gacggtcggg gcggcgtggt
     3061 tctggtacga gcgcttgagc tggtcccggg cccgctggct cgagtttctc gaccggccgg
     3121 aggtcgtcac ttgggtcgcc tatgtggatg gcagccccgc cggctatttc gagctggagg
     3181 atcagccggg cgggtcggtg gagatctgct atttcggcct cctgccggcc tacatcggtc
     3241 agggcatggg cggcgctctg ctgacggatg ccatccgcgc cgcctgggcc aggggcgcgt
     3301 cccgcgtctg gcttcacacc tg
//
LOCUS       DBNHQR010000573         7226 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_499759, whole genome shotgun sequence.
ACCESSION   DBNHQR010000573 DBNHQR010000000
VERSION     DBNHQR010000573.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7226)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7226)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7226
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_499759"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="AC16VR_13075"
     CDS             complement(<1..229)
                     /locus_tag="AC16VR_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012136.1"
                     /translation="MEPVRVLLVTKGHAFDHDAFLAMFEGMDELDVTMVEQPAAQVIL
                     RPENVGDYGAVLFYDMSGIPGIGLMHDGAGDT"
     gene            379..831
                     /locus_tag="AC16VR_13080"
     CDS             379..831
                     /locus_tag="AC16VR_13080"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6012137.1"
                     /translation="MTTIAELLELEEIKNLRHMYSHYFDGKQVDKLADLFTDDAICEF
                     GPDYGGDWVGKEQIRANYATYTEAEGDEHEVMHAVTNPWIRFIDETTANGRWYLLDLR
                     TAEGAENPLILFGIYDDVYKKVDGQWLIHRTRIDFLWPKRHFHGYRDL"
     gene            868..1374
                     /locus_tag="AC16VR_13085"
     CDS             868..1374
                     /locus_tag="AC16VR_13085"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6012138.1"
                     /translation="MEEQLQELLDKKACEEVLLRYGRTQDWLDTPGQASCFWPDAEVD
                     FGFFTGNGEDWVKTVMPHEQEALRRWHMSTSIMIQVDGDRATAECYGIAAGTAEIDGQ
                     LMDTVYGGRYMDELKKRGGEWRISKRTYVLDWSHRFPNALEANTGEDFALHILKIDAP
                     GHPLYREL"
     gene            complement(1371..2246)
                     /locus_tag="AC16VR_13090"
     CDS             complement(1371..2246)
                     /locus_tag="AC16VR_13090"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6012139.1"
                     /translation="MDSVRDKVAFITGAASGIGFGLASVFSREGMRVVMADVEEKALE
                     LAAGALAEGGAEVVPVQLDVTDRDAMARARDRALDAYGAVHLLCNNAGVNAAGPLYRV
                     TYEDWDWVLGVNLGGVVNGVTAFLPELMRHGRDAHVVNTASVGGLVGMPGLGIYNAAK
                     FGVVGLSEALHADLMESGVGVSVLCPGVVRTALTTSDRNRPAHLTSGQATATGESDEP
                     SANEASAMGTEPLDLAEQVLEGVLENRFFLCTHPEFREIVAERNAVLNASFTGTADPA
                     LVEAMRGLVRPIGRG"
     gene            2350..4311
                     /locus_tag="AC16VR_13095"
     CDS             2350..4311
                     /locus_tag="AC16VR_13095"
                     /inference="COORDINATES: protein motif:HMM:NF013199.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR6012140.1"
                     /translation="MRRTLCVALCFAAALLSGCSEQGPESASQAATPAAATEEAEQLQ
                     PGERGAQDYADEIFARESMPGKAVYAQHCAACHDGAVKKAPHKLMLGLMTPEAVLETL
                     STGLMKEQGALLDDEDKRLVSEYLAGVPMGTQAATAVEVCSGAAAEFDFNDPPRTTNW
                     GLQRTNTRSISAADAGLDKASLADLRLEWAFAFPGANRARSQPTLAAGAIYVGSHNGL
                     VYALDRSSGCVRWTYESAAEVRTGIVVDSWNAGDSEAKPRAYFGDVLGNVYGIDAVNG
                     TEIWRMRADEHPNATITGTPSLHDGRLFVPVSSLEVSLAADPAYECCTGRGSVVAVDA
                     ASGEMLWQTYTIEEEPTVQGQNRVETDMYGPSGATVWNSPSIDAKRNQLYVGTGENMS
                     SPATLTSDAIFAMDLETGKVKWVFQATPNDAWNSACDTDRPDSCPVEDGPDFDFGAAT
                     MLLATEKAGDLVIGGQKSGLVHALDPETGKLVWQSRVGRGGIQGGIHFGMASDGKRLY
                     VPISDMRDGRTYPDPERPGLHALDPSTGQELWSFENPDLCTGRDFCHPGISQAITSAG
                     ALVLAGGMDGVMRVHDAATGALLWERDTTETIATLSGARAFGGSFGGGSAPVVRDGLV
                     VLSSGYGIYGHMPGNLLMVLEAEEAPAAD"
     gene            4524..6872
                     /locus_tag="AC16VR_13100"
     CDS             4524..6872
                     /locus_tag="AC16VR_13100"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6012141.1"
                     /translation="MNIRSCGGTAAFVLAGLAAGLLESRPVSAAEEESALVHEEVIVT
                     ARRREESLQTVPVAVSVLTGEELVEQGGMKIDAIGKLVPNVHFEAAGGTSGVKSPIVF
                     IRGMGQNDFIPVEDPAVGIYLDGVYMGRNIGSVFDLVDIERVEVLRGPQGTLFGRNTI
                     GGAVNIISKKPGDEFGGSLKVAGGDEGYLEAQGSVNLPLGDRAAARLSGFVRERDGYV
                     QALQHDDLELGSDDIWGLRGSLRAELTDSFSLDIALDYSKAEETPGAISPIAGIGGFN
                     GDVITLGLPAQPFAFFWNAIWSGVAPPGCTTGTGQASNSACYGPVWNTDDPYKINSSY
                     VDNTGTQIDPEQSVEVWGGNVTATWQIGSVELKSITSYREFDIGFFNDLDFSPYILFA
                     NNHDEYTQEQWSQELQLSGTAMSDRMDYLLGFYYFDEEGHEAIFNQISFAPPLAAPPD
                     FFFQYLDRFIDNDSWAVFGQVNFDLTETLTLTVGGRYTESNKDFALITERRAPAPISV
                     QGGKLTTEEFTPLVSLAWQVQEDVMVYATWSEGYRDGSYAARFTGAVPDPLPNYDPEY
                     VNNYEIGVKTTLLDGRMRLNATAFLMDYEDMQINASSDEVTTSSTKANLGDATISGVE
                     LEISALVTENLTLGLNLGILDDDIDSLKGTLISNTVVIDETSDLPNTPNWTLSLMAKY
                     EIPLNNGSRLAFRADYVAKDDYYSRAENIPELLIDNYENLNVSGTFISPGGDWEVGVG
                     VRNATDEFYYQSATPFATFGLSFGQPVRPRTWYGSLTYNFGN"
     gene            complement(6951..>7226)
                     /locus_tag="AC16VR_13105"
     CDS             complement(6951..>7226)
                     /locus_tag="AC16VR_13105"
                     /inference="COORDINATES: protein motif:HMM:NF024851.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HIR6012142.1"
                     /translation="APGYQLAVHRFRRVPVSSGTLDGVRPPMKVKIDYEKCYKSGECY
                     YNHPELFRMGDEGYPVVLVDEISDDATRRHAEEAIEVCPAVAITLED"
BASE COUNT         1418 a         2356 c         2269 g         1183 t
ORIGIN      
        1 ccgtgtcccc tgccccgtcg tgcatgagac cgatgccggg aatacccgac atgtcgtaga
       61 agagcacggc gccgtagtcc ccgacgttct ccgggcgcag gatgacctgc gccgccggct
      121 gctcgaccat cgtcacgtcg agttcgtcca tcccctcgaa catcgccaga aaagcatcgt
      181 gatcaaacgc gtggcctttc gtgaccagaa gtaccctcac cggctccatt cattcgtcct
      241 ctcttcccgt tagtcgaaac cccgctgcgg acgcggctgc cagaaactaa ccccggcgcg
      301 ggatcgacgc aacggcttga cgcccgcctg cgatacgcac caatctacgt caaggtagac
      361 aggccctggg agggaaccat gacgacgatc gcggagctgc tcgagctcga ggaaatcaag
      421 aacctgcgcc atatgtacag ccactacttc gacggcaaac aggtggacaa gctggccgat
      481 ctcttcacgg atgatgcgat ctgcgaattt ggacccgact acggtggaga ctgggtaggc
      541 aaagagcaga tccgagccaa ctacgcaacg tacacggagg cagaggggga cgagcacgag
      601 gtcatgcacg cggtcaccaa cccgtggatt cgtttcatcg acgagacgac ggccaacggc
      661 cgctggtacc tgcttgatct gcgcaccgcc gagggtgccg agaacccgct cattctgttc
      721 ggcatctacg acgatgttta caagaaggtg gacgggcagt ggctcatcca ccgaacgcgc
      781 atcgactttc tctggccgaa gcgccacttt cacggttacc gggatctctg atctgaaaca
      841 cagaaggacg aaacgatgga cgcgagcatg gaagaacagc ttcaggaact gctggacaag
      901 aaggcgtgcg aggaggtgct gctgcgctac gggcgcaccc aggactggct ggatacgccg
      961 ggacaggcaa gctgcttctg gcccgacgcg gaggtggact tcggcttctt caccggcaac
     1021 ggcgaggact gggtgaagac cgtgatgccg cacgagcagg aagccctgcg gagatggcac
     1081 atgagcacca gcatcatgat ccaggtcgac ggcgatcgcg ccaccgcaga atgctacggg
     1141 atcgctgccg gcaccgcgga gatcgacggc cagctaatgg acaccgtcta tggcggccgg
     1201 tacatggacg agctgaagaa gcgcgggggc gaatggcgca tctcaaaacg gacgtacgtc
     1261 ctggactgga gccatcgatt ccccaacgcg ctggaggcca acaccggcga ggatttcgcg
     1321 ctacacattc tgaagatcga cgcgcccggc caccccctgt accgggagct ttagcctcga
     1381 ccgatcggac gaaccagacc tcgcatggct tccaccaacg cggggtcggc ggttccggta
     1441 aacgacgcat tcagcacggc gtttcgctcc gcgacgatct cacggaactc gggatgggtg
     1501 cagaggaaaa agcggttctc cagaaccccc tcgagcacct gctctgccag gtccaggggt
     1561 tcggttccca tggcggaagc ctcgttggcg ctcggctcgt cgctctcccc ggtcgcggtc
     1621 gcctggccgc tggtgaggtg agccgggcga ttgcggtcgc tcgtcgtcag cgcggtgcgg
     1681 accacgcccg ggcaaagcac cgagaccccg acgccgcttt ccatgaggtc cgcgtggagc
     1741 gcttctgaca gaccgacgac gccgaacttg gccgcgttgt agatccccag gcccggcatt
     1801 cccaccaggc cgcccacgga agcggtgttg acgacgtggg cgtcccgccc gtgccgcatg
     1861 agttccggaa gaaaggcagt cacgccattc accacgccac cgagattgac gccgagcacc
     1921 caatcccagt cttcgtacgt cacccggtag agcgggcccg ccgcgttcac cccggcgttg
     1981 ttgcagagca ggtgcacggc gccgtaggca tctaaagcac gatctcgcgc ccgggccatg
     2041 gcgtcgcggt cggtcacgtc cagctgaacg gggacgacct ccgccccgcc ttccgccagc
     2101 gcgcccgcag caagctcgag ggccttctcc tccacgtcgg ccatcaccac acgcatcccc
     2161 tcccgcgaaa acacgctggc gagcccgaag ccgatgccgc ttgccgcacc cgtaatgaaa
     2221 gccactttgt ctcgaacgga gtccatcgct tgtctccctc ttccacgctc attcctggct
     2281 ggaaactatc attgtccatg ccactgtgat accaatattc gattgggaat ccatggggag
     2341 agaacttacg tgagacgtac gctttgcgtc gcgctctgct tcgcggccgc cttgctgtcg
     2401 gggtgttcgg aacaggggcc cgaatcagca tctcaggcgg caacgccggc cgccgccacg
     2461 gaggaggcag agcagctcca gcccggcgag cggggcgcgc aggattatgc cgacgagatc
     2521 ttcgcccgcg aatccatgcc cggaaaagcc gtttacgccc agcactgcgc tgcctgtcac
     2581 gacggagcag tcaagaaggc gcctcacaag ctcatgctcg ggctgatgac gcccgaggcg
     2641 gttctggaga cgctcagcac cgggttgatg aaggaacaag gcgcccttct ggacgatgag
     2701 gacaagcgcc tggtgtccga gtacctggcc ggcgtgccga tgggaaccca ggcggcgacc
     2761 gcggtggaag tctgctccgg cgcggccgcg gaattcgact tcaacgatcc cccgaggacg
     2821 accaactggg ggctgcagcg caccaacacg cgttccatca gcgccgcgga tgccggcctc
     2881 gacaaagcca gtcttgccga cctcaggctc gagtgggcct tcgcgtttcc aggcgccaac
     2941 cgggcccgat cccagcccac cctggccgct ggcgcgatct atgtgggcag ccacaacggt
     3001 ctggtctacg cgctcgatcg ctcgagcggc tgcgttcgct ggacgtacga gtccgccgcc
     3061 gaggtacgca ccggcatcgt ggtcgacagc tggaacgcag gcgattccga ggcgaagccc
     3121 agagcctact tcggcgacgt gctgggcaac gtctacggca tcgacgccgt caacggcacg
     3181 gagatctggc gcatgcgggc cgacgagcac cccaacgcca ccatcaccgg cacaccgtcg
     3241 ctgcatgacg gacggctctt cgttccggtt tcgtccctgg aggtcagcct ggcggccgat
     3301 ccggcgtacg agtgctgcac cggcaggggc tcggtggtgg cggtggatgc cgccagcggc
     3361 gagatgctct ggcagaccta caccatcgag gaagagccca cggtccaggg ccagaaccgg
     3421 gtcgagacgg acatgtacgg accgtccggg gccacggtct ggaacagccc gtccatcgac
     3481 gcaaagcgca atcagctcta cgtcggcacc ggagagaaca tgtcctcgcc tgccaccctc
     3541 accagcgacg cgatcttcgc catggacctc gaaaccggaa aggtgaagtg ggtattccag
     3601 gcgacgccga acgacgcctg gaactcggca tgcgacaccg accggcccga cagctgcccc
     3661 gtggaagacg gcccggactt cgacttcggc gccgcgacga tgctgctggc cacggaaaaa
     3721 gccggcgatc tcgtcatcgg cggccagaag tcgggtctgg tccacgcgtt ggacccggag
     3781 acgggcaaac tcgtctggca gagccgtgtg ggtcgcggcg gcattcaggg cgggattcat
     3841 ttcggcatgg cgtcggatgg caagcggctc tacgtgccca tcagcgacat gagagacggc
     3901 agaacgtatc cggatccgga aaggcccggc ctgcacgcgc tggatccgag cacgggccaa
     3961 gaattgtggt ccttcgaaaa ccccgacctg tgcaccgggc gcgatttctg ccatcccggc
     4021 atatctcagg ccatcacgtc agccggcgcc ctggtgctgg ccggcggcat ggacggtgtc
     4081 atgagagttc acgacgccgc cacgggggct ctgctctggg agcgcgatac cacggaaacc
     4141 atcgcgaccc tgtcgggcgc ccgcgccttc ggtggatcgt tcggcggcgg ctcggcccct
     4201 gtggtcaggg atgggctggt ggtcctgagc tccggctacg gaatctacgg gcacatgccg
     4261 ggcaatctgt tgatggtgct ggaagcagag gaagcgccgg cagccgactg acccggcccg
     4321 ggcaccgaag tcgatgggct ggcacttccc ggatcggacc atcggttgac ggcaggctca
     4381 atagtcaaca tcattgaccc tcgatccggc caagggaagg aatttgcgcg tcccggtctt
     4441 ggcatggaag acggtgagca ccctcgcgaa cgaacaccgg ggtgctgagg cgatagacaa
     4501 caaagcttcc tggggagggg gttatgaata tcagatcttg cggcggaacc gctgcattcg
     4561 tattggcggg cctggcagcc ggactgctcg agagtcgacc ggtctcagcc gccgaggaag
     4621 agtccgcgct ggtgcatgag gaagtcatcg tcaccgcccg gcgtcgcgaa gagagcctgc
     4681 agacggtgcc tgtggccgtt tcggtgctga cgggagagga actcgtcgag cagggcggca
     4741 tgaagatcga cgccatcggc aagctcgtgc ccaacgtgca tttcgaggcg gccggcggga
     4801 ccagcggcgt caaatcgccc atcgtcttca tccgcggcat gggccagaat gacttcatcc
     4861 cggtcgaaga tcctgccgta ggcatctatc tcgacggcgt gtacatgggc cgcaacatcg
     4921 gcagcgtatt cgacctcgta gacatcgagc gggtagaagt gctcaggggc ccccagggca
     4981 ccctgttcgg ccgcaacacc atcggcggcg cggtcaacat catctccaag aagccgggcg
     5041 acgagttcgg cggctcgttg aaggtggccg gcggagacga gggttacctc gaagcccagg
     5101 gcagcgtgaa cctgcccctg ggcgatcggg ccgcagcccg gctgagcggc ttcgtgcgcg
     5161 agcgcgacgg ctacgtgcag gcccttcagc acgacgatct ggagctcggc agcgacgaca
     5221 tctgggggct gcggggcagc ctgcgggcgg agctgacgga ttcgttcagc ctcgacatcg
     5281 ctctggacta ctcgaaggcc gaggagacgc cgggtgccat ctcgcccatc gccggcatcg
     5341 gcgggttcaa cggcgacgtc attaccctcg ggcttcctgc ccagccgttc gccttcttct
     5401 ggaacgcgat ctggagcggc gttgcaccgc ctgggtgtac gaccggcacc ggccaggcca
     5461 gcaacagcgc gtgctacggc ccggtctgga acacggacga cccctacaag atcaactcga
     5521 gctatgtgga caataccggg acccagatag accccgagca aagcgtcgag gtctggggcg
     5581 gcaacgtcac ggccacctgg cagatcggct ccgtggagct gaagtccatc acctcctatc
     5641 gggagttcga catcggcttc ttcaacgatc tggatttctc gccctacatc ctgttcgcga
     5701 acaatcacga cgagtacacc caggagcaat ggtcccagga gctgcagctg agcggaactg
     5761 ccatgagcga ccgcatggac tacctcctcg gcttctacta cttcgacgag gaaggccacg
     5821 aggccatctt caaccagatc tccttcgcac cgccgcttgc tgcgccgcct gacttctttt
     5881 tccagtacct ggaccgcttc atcgacaacg acagctgggc cgtgttcggg caggtgaact
     5941 tcgacctcac cgagaccctg acgttgacgg taggcggtcg ctacacggag agcaacaaag
     6001 acttcgccct gatcaccgag cggcgtgcgc cggcacccat ctccgtccag ggcggcaagc
     6061 tgaccaccga agagttcacc cccctggtgt cgctcgcctg gcaggtccag gaagacgtca
     6121 tggtctacgc gacctggtcg gaaggctatc gggacggcag ctacgcggcc cgattcaccg
     6181 gcgcggttcc ggacccgctg cccaactacg atcccgagta cgtcaacaat tacgagatcg
     6241 gggtcaagac cacgcttctg gacggccgca tgcggctgaa cgccaccgcg ttcctgatgg
     6301 actacgagga catgcagatc aacgcgtcca gcgacgaggt gacgacgtcc tcgaccaagg
     6361 ccaacctggg agacgcgacc atctccggcg tggagctcga gatctcagcg ctggtaacgg
     6421 agaacctcac cctggggctg aatctgggca ttctcgatga cgacatcgac agcctgaagg
     6481 gaacgctgat ttcgaacacc gtcgtcatcg acgagaccag cgacctgccc aacacgccga
     6541 actggacgct gtccctgatg gcaaagtacg agattccgct caacaacgga tcacgcctcg
     6601 ccttccgcgc cgactatgtg gccaaggacg actactacag ccgcgctgaa aacattcccg
     6661 agctgctgat cgacaactac gaaaacctga acgtcagcgg gaccttcatc tcgcccggcg
     6721 gtgattggga agtgggcgtc ggggtgcgca acgcgacaga cgagttctac taccagtcgg
     6781 ccacgccgtt cgccacgttt ggtctgagct tcggccagcc ggtgaggccg cgaacctggt
     6841 acggatcgct gacctacaac ttcgggaact gaagcatcca gaaagccgcg cccgacgcgc
     6901 tcatcagcga ccgcgtcggg caatttgcct cgtcacacag gtgatggggt tcagtcttcc
     6961 agcgtgatgg ccacagccgg gcacacctcg atggcttcct cggcgtgcct gcgcgtggcg
     7021 tcatcgctga tctcgtccac cagcaccacc gggtagccct cgtcccccat gcggaacagc
     7081 tccgggtggt tgtagtagca ctcgcccgac ttgtaacact tctcgtagtc gatcttcacc
     7141 ttcatgggcg gacgaactcc atccaaagtt cctgaggaga ccggaacccg ccggaatcga
     7201 tggacggcga gctgataccc gggtgc
//
LOCUS       DBNHQR010000574         1467 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1702726, whole genome shotgun sequence.
ACCESSION   DBNHQR010000574 DBNHQR010000000
VERSION     DBNHQR010000574.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1467)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1702726"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="AC16VR_13110"
     CDS             <1..545
                     /locus_tag="AC16VR_13110"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HIR6012143.1"
                     /translation="VVPRDSEARNGTVLRLDPGLAFGTGGHPTTRLCLGWLASHDLAG
                     QRLVDYGCGSGILGIAAALLGAAQVLAVDHDPQALLATEENARANAVDARRLRAVGPD
                     EVPKRGDYDVLVANILANPLLELASRFTDLIRPGGMIVLSGLLARQASEVVAGYPSFR
                     FADPQPDGDWVLLSGRKRDR"
     gene            594..>1467
                     /locus_tag="AC16VR_13115"
     CDS             594..>1467
                     /locus_tag="AC16VR_13115"
                     /inference="COORDINATES: protein motif:HMM:NF023334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HIR6012144.1"
                     /translation="MIEQKLESMITECPHCQTRFRVTDVQLEAAAGRVRCGSCLNVFQ
                     GARHMVPARVPEPAPRRTATVRHLYGGHEAQPPSAEPDESSQVTGARDAAGPPPPPEA
                     DHAARAAEPESADTPSAAGQGAALEVPRSLKKLSAAEAQGLALYAQPPRRRWWAPLAG
                     AAAVLLVATQVLWLQFDTWARDPQVRPLYAMVCGVVGCELPVMRDVESLLAKNLVVRR
                     DPERTDALLVNAIIVNQAAFAQPFPVLELSFTALNGSPVKEERFEPGTYLAGEMAGAT
                     DMPPRTPVHIELTVA"
BASE COUNT          224 a          483 c          526 g          234 t
ORIGIN      
        1 gggtggtccc gcgggattct gaggcgcgca acggaaccgt gctgcgtctg gatccggggc
       61 tggcgttcgg cacgggggga catccgacca cgcggctgtg tctgggatgg cttgcgtctc
      121 acgaccttgc cggccagcgg ctggtggatt acggatgcgg ttccggcatc ctgggcattg
      181 ccgcggcgct gctcggcgcg gcgcaggtgc tcgcggtgga tcacgatccc caggcgttgc
      241 tggcaaccga ggaaaacgct cgggcgaatg ccgtggatgc ccggcggctg agggcggtcg
      301 gaccggacga ggtacccaag cgcggtgact acgacgtgct ggtggctaat attcttgcca
      361 atccgctgct ggagcttgcg agccgcttca cggatcttat ccgaccgggt ggcatgatcg
      421 tgctctccgg gctgcttgcc aggcaggcaa gcgaggtcgt agcagggtat ccttcattcc
      481 gcttcgccga cccccagcct gacggcgact gggtgttgct ctcagggcgc aagcgtgacc
      541 ggtgactgat cgaagccgca ggcagggtcg agggacggac gaagtctaaa gtcatgattg
      601 agcagaagct ggaatcgatg atcacggagt gcccgcactg ccagactcga ttccgggtga
      661 cggacgtgca gctcgaggcg gctgcaggga gagtgcgttg cgggtcctgc ctcaacgtct
      721 tccagggcgc gcgccacatg gtgcctgccc gcgtcccgga gccggccccg cgtcgaaccg
      781 ctaccgtgcg ccacctctac ggaggccacg aggcgcagcc gccttcggcg gagccggacg
      841 aatcctcgca agtcacgggc gcccgggatg cggcgggccc gcccccgccc cccgaagccg
      901 accatgcagc gcgggcggca gagcccgaaa gcgccgatac tcctagcgca gccgggcaag
      961 gcgctgccct ggaggtcccc agatccctca agaagctctc cgcggcggag gcccaggggc
     1021 tagccctgta tgcgcagccg ccgcgcagac gctggtgggc gccgctggcc ggggcggccg
     1081 cggtgctgct ggtggcgacc caggttctct ggcttcagtt cgacacctgg gcgcgcgacc
     1141 cccaggtgag gccgctgtat gccatggtat gcggcgtcgt gggctgcgag ctgccggtga
     1201 tgcgcgacgt cgagagcctg ctcgccaaga atctggtggt gcggcgcgac ccggagcgaa
     1261 ccgatgccct gctcgtcaac gccatcatcg tcaaccaggc cgccttcgcg cagccctttc
     1321 ccgtgctcga gctgagcttt accgcgctca acggctcgcc ggtcaaggag gagcgattcg
     1381 agccgggaac ttatctggca ggggagatgg ccggggccac cgacatgccc ccgcggacgc
     1441 ccgttcacat cgagctgacc gtcgcgc
//
LOCUS       DBNHQR010000575         5250 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1512612, whole genome shotgun sequence.
ACCESSION   DBNHQR010000575 DBNHQR010000000
VERSION     DBNHQR010000575.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5250)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5250)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5250
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1512612"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="AC16VR_13120"
     CDS             complement(<1..190)
                     /locus_tag="AC16VR_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012145.1"
                     /translation="MSTLAVAFTAGGAHAQPDSGASHQHAAPVVLAPGYADLDFAPPP
                     VGGYALPPLGEVADARVVD"
     gene            complement(215..1552)
                     /gene="mtoX"
                     /locus_tag="AC16VR_13125"
     CDS             complement(215..1552)
                     /gene="mtoX"
                     /locus_tag="AC16VR_13125"
                     /EC_number="1.8.3.4"
                     /inference="COORDINATES: protein motif:HMM:NF052453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008430 - selenium binding [Evidence IEA];
                     GO_function: GO:0018549 - methanethiol oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanethiol oxidase"
                     /protein_id="HIR6012146.1"
                     /translation="MKTIRKMLAQLAAGALLAMPVWADETCMSPYMAKIVGQEDFVYV
                     WTLGMEGVGDGQDKLVTVDVNPTSKTYGEVVHSLSVGGRNEAHHSGLTDDRRFLWASG
                     LDTSKIFIFDVHTNPGAPTLHRMIDDFVARSGGVVGPHTNYALPGRMMLTGLSNNRDH
                     GGRTALVEYTNAGDYIATHWMPTDDNLQGAVKSGSYADGYGYDVRVLPRRNVMITSSF
                     TGWNNYMMNLGAMMADAEAMKRFGNTVVVWDLHARQPKKILDVPGAPLEIRCAWGAKS
                     NYCFTSTALTSKIWLIYEDEGGEWQAKAVADIGDAAKIPLPVDISISAEDNLLWVNTW
                     NDGLVHLYDISDPHAPKQLFEQKIGEQVNMVSQSWDGERLYFTSSLLANWDKTGPSGP
                     DLQYFKLYGWDGEKMTPGFTIDFVAAGLGAPHQMRFGSYALYGKRPPQQVGLH"
     gene            1621..2850
                     /locus_tag="AC16VR_13130"
     CDS             1621..2850
                     /locus_tag="AC16VR_13130"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004130 - cytochrome-c peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HIR6012147.1"
                     /translation="MWRGPITSQAVAALMLTACCTWAVTPGDPRDGYEHADYETASRQ
                     LSALRGSALALAELAGNPPLGLPPLPAERLPVAAAIDLGRRLFFDRRLSANATLSCAM
                     CHVPEQGFTQNELKTPVGIEGRFVHRNAPSLYNVAYQEALFHDGREDDLANQIWAPLL
                     AANEMGNASREAVVERLGGLEEYEGEFLRVFGDGISAATLGRALASYQTALLSADSPF
                     DRWYFGGEADAVGAEVKRGFRSFVNEGCATCHQLQTGHATFTDGAYHDTGIGFLAAAS
                     AGTPARRLQIAPGVIIDVETDIATPHVEDDGRAAVTGDPADRWRYRTPGLRNVALSAP
                     YMHDGSLATLEAVIDYYAAGGAPHDGQDARIRPLSLGADERADLVAFLRALTGSNVDA
                     LAGDARVAPIGDTHHGP"
     gene            complement(2866..>5250)
                     /locus_tag="AC16VR_13135"
     CDS             complement(2866..>5250)
                     /locus_tag="AC16VR_13135"
                     /inference="COORDINATES: protein motif:HMM:NF012361.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR6012148.1"
                     /translation="RDPQLGADVYMAAQRVADYGSFEEVGRVKGRELVGRRYEPLFPY
                     FREAGEEGAFVVVMDDYVTTESGTGLVHQAPAFGEDDYRIARAAGIDALVCPVTVNGE
                     FTDEVTDFAGQHVKDADRDIIRYLKDHNLLYRQDVIQHSYPYCYRSDTPLIYRAIPSW
                     YVRVTEFVDKLVKNNQQIRWVPDHIKTGRFGNWLEGAIDWSISRNRVWGTPIPIWIND
                     VTGNEVCIGSIDELEALTGVRVDDLHREHVDPLTFTLQGEEGTYHRIEEVLDCWFESG
                     SMPYAQLHYPFENEKVFKAGFPAEFIAEGLDQTRGWFYTLTVLAAALYDKPAFRNVIV
                     NGMVMAEDGKKMSKRLRNYTPPDELMETYGADALRLYLINSGLMRGEEQRFADAGVRD
                     MTRRALLPWYNAFTFLKTYAEIDQWSPDKGQHTGDNVLDHWILSRLQTLKANVGREME
                     VYKLYNVVPQLFDFIEDLTNWYIRLNRQRFWGEDITADKIAAYSTLYAALYELCQVMA
                     PFAPFLAEHVYQELSALGGGRAKPESVHLCDYPEPEGALIRSELEEAVDRMQQVVLLG
                     RQKREEVKIGLRTPLRRLTIVNRDRALLEELARLESYVKTELNVLAVSYDDDEGAYIE
                     VVAKPNFPVLGKRLGKRMKAFQSAIATLSPEQIAAFQAEGEIELDGEVFNLEEIEVLQ
                     KARPGTNTISNSRIAVDLDCVLDDELVRGGYAREIVNRIQRRRKELELNVADRIEVCF
                     AGDRALVEAAAEHRDYIAAETLATDFAEGDPGSQAADSEIDGKAFRFSVVRAGS"
BASE COUNT          894 a         1754 c         1670 g          932 t
ORIGIN      
        1 catcgaccac cctggcatcg gccacttccc ccaggggcgg cagcgcgtag ccgcccacgg
       61 ggggcggggc gaagtccaga tcggcgtatc ccggcgcaag caccaccggc gcggcatgct
      121 ggtgcgaagc gccggagtca ggctgcgcgt gagctccacc ggcggtgaaa gccaccgcca
      181 gcgtgctcaa caggcaggca cgccatccca ccggtcaatg cagtcccacc tgctgcggcg
      241 ggcgtttgcc gtagagcgcg tatgagccga agcgcatctg gtgcggcgcg cccagcccgg
      301 ccgccacgaa atcaatcgtg aaccccggcg tcatcttctc gccatcccag ccgtagagct
      361 tgaagtactg caggtccgga ccgctgggtc ccgtcttgtc ccagttggcc agcagcgagg
      421 aggtgaagta gagccgctcg ccgtcccagc tctgggacac catgttcacc tgctctccga
      481 tcttctgctc gaacagctgc ttcggcgcgt ggggatcgga gatgtcgtag aggtgcacca
      541 gaccgtcatt ccaggtattg acccagagga ggttgtcttc cgcagagatc gaaatgtcca
      601 ccggcagcgg gatcttggca gcatcgccga tatccgctac ggccttggcc tgccactcgc
      661 cgccctcgtc ctcgtagatc agccagatct tggaggtcaa cgccgtggag gtgaagcagt
      721 agttgctttt ggcgccccag gcgcagcgaa tctccagcgg ggcgccggga acgtcaagaa
      781 tcttctttgg ctgacgggca tgcagatccc acacgacgac cgtgttgccg aagcgcttca
      841 tcgcctccgc atcggccatc atggcgccta agttcatcat gtagttgttc caccccgtga
      901 acgaggacgt gatcatcacg ttgcgccggg gcagcacccg aacgtcgtag ccgtaaccat
      961 cggcgtagct gccggacttg acggcgccct gcaggttgtc gtccgtgggc atccagtgag
     1021 tggcgatgta atcccccgcg ttggtgtact cgaccagcgc cgtgcggccg ccgtggtcac
     1081 gattgttaga caggccggtg agcatcatcc ggcccggcag cgcgtagttg gtgtgcgggc
     1141 ctaccacccc tccgcttctg gccacgaaat cgtcgatcat ccggtgcagc gtgggggcgc
     1201 cggggttggt gtgcacgtcg aagatgaaga tcttgctggt atccagaccg ctcgcccaga
     1261 gaaagcgccg gtcatcggtc aggcccgagt gatgcgcctc gttgcggccg cccaccgaca
     1321 ggctgtgcac cacctctcca taggttttcg aggtcgggtt gacgtccacg gtcacgagct
     1381 tgtcctgacc gtcgcccacg ccctccatcc cgagcgtcca gacgtaaacg aaatcttcct
     1441 gcccgacgat cttggccatg tagggtgaca tgcacgtctc gtctgcccag acgggcatgg
     1501 cgagcaacgc gcccgccgcc agctgcgcga gcatcttcct gatagtcttc atcggttccc
     1561 cctgttctgg aacggtctcg caggtatcct atcaccccaa tggagtcggt cctaagacac
     1621 atgtggcgag gcccgatcac cagtcaggca gtagccgccc tcatgctgac cgcgtgctgc
     1681 acctgggccg tcacgcccgg tgatccgaga gacgggtacg agcacgccga ctacgagacg
     1741 gcgtcgaggc aactcagcgc actccgcgga tcggcgctgg cgctcgcgga gctggcaggc
     1801 aacccgcctc tcggcttgcc gcccctgccg gcagagcggt tgccggtggc ggcggccatc
     1861 gacctcggcc gacggctgtt cttcgacagg cgactgtctg ccaacgcgac gctgtcctgc
     1921 gccatgtgtc acgtgccgga gcagggtttc acccagaacg agctgaaaac cccggtgggc
     1981 atcgaaggcc gcttcgtgca ccgcaacgca ccctcgctct acaacgtggc gtaccaggaa
     2041 gccctgtttc acgacggccg ggaagacgac ctggccaacc agatctgggc cccgctgctg
     2101 gcagccaacg agatgggcaa cgcctcccgc gaggccgtgg tcgagcgcct ggggggcctg
     2161 gaggagtacg agggcgagtt tctccgggtg ttcggcgacg ggatcagcgc cgcaacactg
     2221 ggtcgggcgc tggccagcta ccagacggct cttctgtcgg ccgactcccc cttcgaccgc
     2281 tggtacttcg gcggcgaggc cgatgccgtc ggcgcggagg tcaaacgcgg atttcgatca
     2341 ttcgtcaatg aaggttgcgc gacctgccat cagctgcaga cgggccacgc cacgttcacg
     2401 gacggggcct accacgatac cggcatcggc ttcctggcag cggccagtgc gggcacgccg
     2461 gcccgccgcc tgcagatcgc gcccggggtc atcatcgacg tcgaaacgga catcgcgacc
     2521 ccgcacgtcg aggacgacgg ccgagcagcc gtgacggggg atccggccga ccgctggcga
     2581 taccgaacgc cgggtttgcg caacgtggcg ctgagcgcgc cttacatgca cgacggctcg
     2641 ctcgccaccc tggaggcggt catcgactac tacgcagcag ggggcgctcc ccacgacggc
     2701 caggacgccc gaatcaggcc gctgtctctg ggcgctgatg aacgcgccga cctcgtcgcc
     2761 ttcctgcggg cgctgacagg cagcaacgtc gacgccctgg ctggcgacgc cagggtcgcg
     2821 cccatcggcg acacgcatca cggtccttga gcgcggtctg agtgctcagc tcccggcgcg
     2881 gacgacggag aagcggaacg ccttgccatc gatctcggag tccgccgcct gggatcccgg
     2941 atcgccctcg gcaaagtcgg tggccagagt ctccgcagcg atgtagtcac ggtgttcggc
     3001 ggccgcctcg accagagccc gatccccagc gaagcagacc tcgatgcgat ccgccacgtt
     3061 cagctccagc tccttgcgcc ggcgctgaat gcggttgacg atctcgcggg cgtaaccgcc
     3121 gcgcacgagc tcgtcgtcca gaacgcagtc cagatcaacg gcgatacggc tgtttgagat
     3181 ggtgttggtc ccaggtcgcg ctttctgcag cacctcgatc tcctcgaggt tgaagacctc
     3241 cccgtcgagc tcgatctcgc cctcggcctg aaaggcggcg atctgctcgg gagacaaggt
     3301 cgcaatcgcg ctctgaaagg ccttcatccg cttgcccagc cgcttgccga gcacgggaaa
     3361 gttcggcttg gcgaccacct cgatgtacgc gccttcgtcg tcatcgtagc tcaccgccag
     3421 gacgttgagc tccgttttga catagctctc cagccgggcg agctcctcga gcagcgctcg
     3481 gtcgcggttg acgatggtca ggcgtctgag cggcgtgcga agaccgatct tcacctcctc
     3541 gcgcttctga cgaccgagca gcacgacctg ctgcatgcgg tcgacggctt cttccagctc
     3601 gctcctgatc agcgcgccct ccggctccgg ataatcgcac agatgcaccg actcgggctt
     3661 ggcccggccg ccgcccagcg ccgacagctc ctggtacacg tgctccgcaa ggaacggcgc
     3721 gaaaggcgcc atcacctggc agagctcgta cagcgcggcg tagagcgtgc tgtaggcggc
     3781 aatcttgtct gccgtgatgt cctcgcccca gaaccgctga cggttgaggc ggatgtacca
     3841 gttggtcagg tcctcgatga aatcgaacag ctgcgggacc acgttgtaga gcttatagac
     3901 ctccatctcc cggccgacgt ttgccttgag ggtctgcagg cgcgagagga tccagtgatc
     3961 caggacgttg tcgccggtgt gctgaccctt gtccggcgac cactgatcga tctccgcgta
     4021 ggtcttcagg aaagtgaacg cgttgtacca gggcagcagg gcccggcgcg tcatgtcccg
     4081 cacgccggca tcggcgaaac gctgctcctc cccccgcatc agccccgagt tgatgagata
     4141 caggcgcagg gcgtccgcgc cgtaagtttc catgagctca tcgggcgggg tgtagttgcg
     4201 aagccgcttg gacatcttct tgccgtcctc cgccatcacc atcccgttga cgatgacgtt
     4261 gcggaaagcg ggcttgtcgt agagcgcggc ggctagaacc gtcagcgtgt agaaccagcc
     4321 tcgcgtctgg tccagccctt cggcgatgaa ctcggccgga aagccggcct tgaagacctt
     4381 ctcgttctcg aacggatagt gcagctgggc atagggcatg gagccagact cgaaccagca
     4441 gtccagcacc tcctcaatcc gatggtaggt gccttcctcg ccttggagcg taaaggtcag
     4501 cggatccacg tgctcgcggt gcagatcatc gacgcggacg ccggtgagcg cttccagctc
     4561 gtcgatggag ccgatgcaga cctcattgcc cgtgacgtcg ttgatccaga tgggaatcgg
     4621 cgtgccccag acccggttgc gggagatgga ccagtcgatg gcgccttcca gccagtttcc
     4681 gaaccgcccg gtcttgatgt ggtcgggaac ccagcggatc tgctggttgt ttttgacaag
     4741 cttgtcaacg aactccgtta cccgcacgta ccaggacggt atggcccgat agatcagggg
     4801 cgtatcgctg cgatagcagt aggggtagct gtgctggatc acgtcctggc ggtagagcag
     4861 gttgtgatcc ttcagatagc ggatgatgtc gcgatccgcg tccttgacat gctgcccggc
     4921 aaagtcggtg acctcgtcgg tgaactcgcc gttgacggtt accggacaga ccagggcatc
     4981 gataccggcg gcccgcgcga tacggtagtc gtcctcgccg aaggccggcg cctggtgcac
     5041 caggccggtg ccgctttcgg tggtgacgta gtcgtccatc accaccacga acgcgccctc
     5101 ctcgcccgcc tcgcggaaat aggggaagag cggctcgtag cggcgaccca cgagctcgcg
     5161 ccctttgacg cgccccacct cctcgaagct gccgtagtcg gctacgcgct gcgccgccat
     5221 gtagacgtcg gcgcccagct gcgggtcccg
//
LOCUS       DBNHQR010000576         2513 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_753478, whole genome shotgun sequence.
ACCESSION   DBNHQR010000576 DBNHQR010000000
VERSION     DBNHQR010000576.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_753478"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="AC16VR_13140"
     CDS             complement(<1..536)
                     /locus_tag="AC16VR_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR6012149.1"
                     /translation="MHTDRSRELMRRAALSIPGGVNSPVRAFKGVGGSPIFFERGEGA
                     FLTDVDGNRYIDYVGSWGPMLLGHGNPEVVAAVRAQAEKALGFGAPTELEIELAEQVV
                     DMVPSIEKIRMVNSGTEATMSAIRLARGFTGRDLIVKFDGCYHGHSDSLLVKAGSGVL
                     TLGIPNSPGVPAAIAEQTL"
     gene            complement(564..998)
                     /gene="hemJ"
                     /locus_tag="AC16VR_13145"
     CDS             complement(564..998)
                     /gene="hemJ"
                     /locus_tag="AC16VR_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007251893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HIR6012150.1"
                     /translation="MSAGYLWLKALHIIFVVTWFAALFYLPRLFVYHSSSTDAISRDR
                     FKVMESKLYRVIMRPSMLLATGFGLWLVFLGWNAFAGSGWLWVKLTAVVALLGYHHYC
                     ARLVRAFAADADPHSETFYRWFNELPALVLIVVVIMVVVKPF"
     gene            complement(1007..>2513)
                     /locus_tag="AC16VR_13150"
     CDS             complement(1007..>2513)
                     /locus_tag="AC16VR_13150"
                     /inference="COORDINATES: protein motif:HMM:NF012861.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HIR6012151.1"
                     /translation="IAGAGLIGLALNRLSAAERRVGVVHVMERLSRHQGYLPWRNAAV
                     QFFGGIAALASGQSGGREGPAIHLGAASSSLIGQIFRLPNNSLRTLVACGSAAAIASS
                     FNTPIAGVIFAMEVVMMEYTIGSFIPVIIAAVTSTLLTHYFIGDAPAFTVAPLQMQSL
                     SEVPFVVLAGIVIGCTAAGFTALVQLFARLSRWPFWSRALLAGALTGLAATMVPAVMG
                     IGYDTVNAAMLGQLSLTALLLVALFKSISSAAAVGLGLPVGLIGPTLVIGAALGGCIG
                     MIGNLLEPADAISEGFYVMLGMAAMMAAVLQAPLAALMAVMELTLNPNLRLPAMLMIV
                     VATLVSSVAFRQRSVFISTLKTLGLEYPPSPVTAHMQRAGVAAIMCEEVAVLPRDCSL
                     DAARRALEQRPLWIAIASDDGETRWVLKSSDLAAHLESEQIDTADVDLLNIPGMRRDA
                     TSVDVRCTVDEAQMALQGSPAEALCVRRLGVTAAGSLLGIVTQEDIDTYRG"
BASE COUNT          470 a          812 c          839 g          392 t
ORIGIN      
        1 agcgtctgct cggcgatggc cgcgggtacc ccgggtgagt tcgggatgcc cagcgtcaga
       61 acgcccgagc ccgccttgac cagcagggag tcggaatgcc cgtgataaca gccgtcgaac
      121 ttaacgatga ggtcgcgccc ggtgaagcct ctggcgagcc ggattgccga catggtggcc
      181 tcggtgcccg agttcaccat ccgtatcttc tcgatgctcg gcaccatgtc cacgacctgc
      241 tccgcgagct cgatttcgag ctcggtgggc gcgccgaagc ccagagcttt ctcggcctgg
      301 gccctcacgg cggcgacgac ttcggggttg ccatgcccta acaacatcgg gccccaggag
      361 ccgacgtagt cgatgtaacg gttgccgtcc acgtctgtga gaaacgcgcc ctcaccccgc
      421 tcgaagaaaa tgggcgagcc gccgacgccc ttgaacgcgc ggacggggga gttgacgccg
      481 ccgggaatgg acagggccgc ccgccgcatc agttccctcg acctgtctgt gtgcatcgct
      541 cgacccctat cgcgtgacgg gagctagaag ggtttgacca ccaccatgat gaccaccacg
      601 atcagcacga gtgcgggcag ctcattgaac caccggtaga acgtctcgct gtgggggtcg
      661 gcatctgccg caaaggctct gacgagccgc gcgcagtaat gatgataacc gagcagtgcg
      721 accaccgcag tcagtttcac ccacagccag cccgagccgg cgaacgcgtt ccagccgagg
      781 aagacaagcc agaggccgaa gccggttgcc agcagcatgg agggccgcat gatgacgcgg
      841 tagagcttgc tctccatcac cttgaatcgg tcgcggctga tcgcatcggt gctgctggag
      901 tggtagacga acagcctcgg caggtagaag agggccgcga accaggtcac gacgaagatg
      961 atgtgaaggg ccttcagcca caggtagccg gcgctcatcg tttggatcaa cctctgtagg
     1021 tgtcgatgtc ttcctgggtg acgatgccca gcaggctccc ggccgccgtc acgcccagcc
     1081 ggcgcacgca cagcgcttcg gcagggcttc cctgcagggc catctgggct tcgtcaacgg
     1141 tgcagcgcac gtcgacgctg gtcgcgtccc tacgcatgcc gggaatattg agcaggtcga
     1201 cgtcggcggt gtcaatctgc tcggattcta ggtgggcggc caggtcgctc gacttgagga
     1261 cccagcgggt ctcgccgtcg tccgatgcga tcgcaatcca taacggcctt tgctcgagtg
     1321 cccttcgcgc ggcgtcgaga gagcagtcac ggggaagcac cgccacttct tcgcacatga
     1381 tggccgccac gccggcgcgc tgcatgtgcg ccgtgaccgg gcttggcggg tactcgagcc
     1441 ccagggtctt cagggtggag atgaacaccg atcgctgcct gaacgccacg ctgctcacca
     1501 gcgtcgccac cacgatcatc agcatggcgg gcagtcttag attcgggttc agggtgagct
     1561 ccatgacggc catgagcgcg gcgagcggag cctgaagcac ggcggccatc atggccgcca
     1621 tgccgagcat gacgtagaac ccctcggaga tcgcatcggc cggctcgagc aggttgccga
     1681 tcatgccgat gcagccgccg agagccgcgc cgatgaccaa cgtgggaccg atgaggccaa
     1741 cgggcaggcc caggcccacc gccgccgcgc tgctgatcga cttgaacagc gcgacgagca
     1801 gcagcgccgt aaggctcagc tgtcccagca ttgccgcgtt gaccgtgtcg tagccgattc
     1861 ccatcaccgc cggcaccatg gtagcggcaa gcccggtcag ggctccggca agcagggctc
     1921 ggctccagaa gggccagcgg gagagacgcg cgaaaagctg aactagcgcg gtgaatcctg
     1981 ccgccgtgca gccgatgacg atcccggcca gcacgacgaa gggcacttcc gacagggact
     2041 gcatctgcag tggagccaca gtgaatgcgg gcgcgtcgcc aatgaaatag tgggtcagca
     2101 gggtggaagt gacggcggcg atgatgaccg ggatgaagct gccgatggtg tactccatca
     2161 tgacgacctc catcgcgaag atgacccccg cgatgggcgt attaaaggag ctggcgatgg
     2221 cggccgcgct gccgcaggcg accagggtgc gcaggctgtt gttgggtagc cggaagatct
     2281 ggccgatcag gctcgagctt gcggcgccaa gatgaatcgc gggtccttcc cttcccccag
     2341 actgaccgga cgccagggcc gcgatgccgc cgaaaaactg caccgcggcg ttgcgccacg
     2401 gcaggtagcc ctggtgccgg gagagccgct ccatgacgtg gacgacgccc acccgccgct
     2461 ccgccgcgga caggcggttg agcgccagcc cgatcaggcc ggcgccggcg atg
//
LOCUS       DBNHQR010000577         9822 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1069954, whole genome shotgun sequence.
ACCESSION   DBNHQR010000577 DBNHQR010000000
VERSION     DBNHQR010000577.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9822)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9822)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9822
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1069954"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2408)
                     /locus_tag="AC16VR_13155"
     CDS             complement(<1..2408)
                     /locus_tag="AC16VR_13155"
                     /inference="COORDINATES: protein motif:HMM:NF024516.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HIR6012152.1"
                     /translation="MHRIVLRPFLVVVTLTMLFVALMQGTGRLGFMFLPDLELVANQW
                     LSGQRVRVSGLEGSWRYLNPVVRAARVDLPAGHLREVVVELDWLESLVRNRFVARRVL
                     VGEGAVRLEKTPAGKWRLAGTSGDGEFDASGIVYHADELRFSGVIAFGLAGEEEAQSV
                     QVDYLATNRGGLHRHRLHLDNQDADCAADCGLHLEYQARDGRWPFWDERIQLSLDGGG
                     FVIPAAVLDSPLELMSIGIDWHLRGDESGGRLHAHARGLRLPGGVELAGLVRADVRGF
                     EDVHDAVVHDLAFGLVAHLDDSEEQVRTQRALRMPAVHVRKDAEVVQFWAQRVDMGGV
                     SEFLERALRGSERARRWLSALKLQAEALNVRGYYRLPTGESGFAATLAGLDVDGYRGA
                     PFVRGAAGELLGHARGLELKLKSEDVLLKFPQTFTDTWQMPYLQGAFQAWFGNGYLGL
                     RGVNLRAETLFSRAAGSFALTRPPGERYEERLSLLIGVDALDTDQAFTFVPYTIDEGL
                     EDWLRGGLKAGLLTRIRFGYHGQQHLKGNELARRIELSARISDGRIAYAPDWPDVHEL
                     DADVVVAGPMVRVTVDKGASEGVRLGGSQVVLRNNASYADVELVSRMETDLALDFVRT
                     TPLAESMGFVAPEWVGDGRLEVLGNLHIPIKLPEDVDASEQLQVDVNVRMLDAGLRMP
                     DFRLTLGDLEGDLRYRYPNGLRSSRISGTLFGNDVLIDADSNSQEMILSFAGRATHED
                     VLAVLDMEDPGVVTGSADFLASLHVGVDPDHVTRLEVVSDLQGMALDLPADLRKPAEV
                     ARPV"
     gene            complement(2547..4040)
                     /locus_tag="AC16VR_13160"
     CDS             complement(2547..4040)
                     /locus_tag="AC16VR_13160"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HIR6012153.1"
                     /translation="MSEELLINVNDFESRVALVSGSVVEEVQVARAGADSLTGNIYLG
                     RVQRIIPGMQAAFVDCGLERPGFLHVSDIAGPRLMLGEEPDGAVDIRELLHDGQQLMV
                     QVAKDPIASKGARLTTQLTIASRYLVLMPFSDHIGISQRVDEEDERERLRLLVDEVRG
                     GQDAGVGFIVRTAADGVDREPLEADVRVLLRIWDRIMARRAECGCPGIVYEELPLHTR
                     VIRDMATAELECIRIDHPPTLERVRKFVAEFLPELTDKVVLYDEPRPIFERFGVDDEL
                     TRALGSRVELKSGGHLVIEQTEAMTTVDVNTGAYLGKHNLEETVFRTNLEAAQVLPRQ
                     LRLRNLGGIIVVDFIDMEDEEHRRQVLRALEKACEGDPARIRIEGFSSLGLVQMSRKR
                     TRESLVQQLCEPCSHCHGAGVVKTAQSTCIEIFRSIMQDAESGCGAHSQGDYVIRAAD
                     NVVDRLLDEDADRLAAMAARVGRRVRIELEPSYGAGEFDIVLVGDTQ"
     gene            complement(4033..4605)
                     /locus_tag="AC16VR_13165"
     CDS             complement(4033..4605)
                     /locus_tag="AC16VR_13165"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007998187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HIR6012154.1"
                     /translation="MVLASGSPRRAELLRQIGLPFVVRPPDVDESLRPGESPERYVER
                     LAIGKARAVVAEDGPALGADTAVVVDEEILGKPADRASAIDMILRLSGRTHRVLTGVA
                     VTDGRAVRSCVVAADVTFRAITRDEAAAYWETGEPRDKAGGYGIQGIGGIFAQSINGS
                     YSAVVGLPLAETEALLHAIGVNTWELRASV"
     gene            complement(4680..5174)
                     /gene="mreD"
                     /locus_tag="AC16VR_13170"
     CDS             complement(4680..5174)
                     /gene="mreD"
                     /locus_tag="AC16VR_13170"
                     /inference="COORDINATES: protein motif:HMM:NF016019.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HIR6012155.1"
                     /translation="MNPAQGGWLILASLVVALVLSIFHLPETWPQWLGWLRPAWVPLF
                     VFFWVMALPHRLGLVSAWIFGIFVDVLFGDPIGLNGAVLAGITYIGWRFYERLRMYSV
                     VQQCGVVFLLILGGDLVRMLVHDLSADRGWSWAVLVPALTSMLVWPFVSLFLQRLRLQ
                     FGVE"
     gene            complement(5171..6058)
                     /gene="mreC"
                     /locus_tag="AC16VR_13175"
     CDS             complement(5171..6058)
                     /gene="mreC"
                     /locus_tag="AC16VR_13175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HIR6012156.1"
                     /translation="MATRTCCRPNRETQAIKALFVRESGAGFLLTGLVLVSLILVTLD
                     LTTSFMRGTRQVFGTMLAPLHYVAETPYLVGEELGNVFATRSALLDENQMLKRQVLEL
                     SKISLQYRNLLKENENLRELLGSRARLPDEVLIAEIIGIVPTVGAHQVVIDKGSDAGV
                     QIGQAVIDSEGLFGQVVEVDVYSSRVLLITDRDHAVPVQINRNGIRSIAGGTGRMDQL
                     ELENVTITADVVKGDLVETSGLGGRFPEGYPVGEVESVVVDPAASLSRVLVRPSALLD
                     RSRHVLVVFAPETRDEVLP"
     gene            complement(6027..7067)
                     /locus_tag="AC16VR_13180"
     CDS             complement(6027..7067)
                     /locus_tag="AC16VR_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HIR6012157.1"
                     /translation="MFKNIRGLFSRDLSIDLGTANTLIYVRDQGIVLNEPSVVAIRVQ
                     GNQKSVAAVGLDAKRMLGRTPGNITAIRPLKDGVIADFLVTEKMLKYFIAKVHENSFI
                     RPSPRVLVCVPCKSTEVERRAIRESALSAGARDVRLLEEPMAAAIGAGLPVQEAVGTM
                     VVDIGGGTTEIAIISLNGVVYSDTVRVGGDRFDESIVAYVRRTQGSLIGEATAERIKQ
                     EIGAAYPQKEVREIDVRGRNLAEGVPRSFTLNSNEILEALQEPLSMIVQALKSALEQC
                     PPELASDIAETGLVLTGGGALLRDLDTLLAEETGLPVIVAEDPLTCVARGGGKALELM
                     DKAGNADLLSTE"
     gene            7237..7518
                     /gene="gatC"
                     /locus_tag="AC16VR_13185"
     CDS             7237..7518
                     /gene="gatC"
                     /locus_tag="AC16VR_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003120927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HIR6012158.1"
                     /translation="MASIEIAHLCRLAQLALTESEQAAALADLQRIIAMIDEMQAMDT
                     DGVEPLAHPIESDQRLRPDTVSETVDRDHYQRGAPATRDGLYLVPRVVE"
     gene            7520..8977
                     /gene="gatA"
                     /locus_tag="AC16VR_13190"
     CDS             7520..8977
                     /gene="gatA"
                     /locus_tag="AC16VR_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HIR6012159.1"
                     /translation="MDPFASIGQHAARLRDGSYSSVELTRFFVDRAREHADLNAFICV
                     DEEGALAAAEAADARLRAGTATALTGIPLAHKDIFCTRGTITSCASKMLENFVAPYDA
                     TVVERLNAAGAVTVGKANMDEFAMGSSNENSRFGPVRNPWDPGRVPGGSSGGSAAAVA
                     AGLVPVASGTDTGGSIRQPSAFCGVSGIKPTYGRVSRYGMVAFASSLDQGGFLGRSAE
                     DLALSLAFCAGADPRDSTCSEHVDEWLDGVAENGVGNLEGGLRIGLPREYFADLSTGS
                     ELLDAVAGFFESIGCSLEEVSLPHTSAAIPAYYIIAGAEASTNLSRYDGVRFGYRCEN
                     PGSLEDLYRRSRSEGFGAEVKRRILTGTYALSVGYYDAYYVKAQRLRRLIQRDFLEAF
                     ETVDMILAPTAPGVAFPLGELTHDPVAMYQQDIFTIPASLAGLPAMSLPCGFHQGLPI
                     GVQLIGPHFAEKTLLQAGAAYQRETDWHTRHPEAG"
     gene            8979..>9822
                     /gene="gatB"
                     /locus_tag="AC16VR_13195"
     CDS             8979..>9822
                     /gene="gatB"
                     /locus_tag="AC16VR_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020292891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIR6012160.1"
                     /translation="MGFRIPQGWEAVIGLEVHVQLATRSKIFSGASTRFGAEPNTQAC
                     PVDLGLPGVLPVLNAEAVRMAVTFGLAVGAEIRPYSVFDRKNYFYPDLPKGYQISQLP
                     HPIVGRGAITIEASGEAPRTIGITRAHLEEDAGKSIHDAYAGLTGIDLNRAGTPLLEV
                     VSEPELRTPEEAGAYFRKLHSLVRHLKICDGNLAEGSMRCDANVSVRPAGETALGQRT
                     EIKNVNSFRFLERAIEFEIERQIDVLEAGGAIEQQTLLYDPDSHETRPMRTKEYSEDY
                     RYFPD"
BASE COUNT         1747 a         3441 c         3042 g         1592 t
ORIGIN      
        1 tcaaccggtc gggcgacctc cgcgggcttg cgcagatcgg ccggaagatc cagcgccatg
       61 ccctgaagat cggagacgac ctccagccgg gtgacgtgat ccggatccac gcccacgtgc
      121 agcgaggcca ggaagtcggc gctgccggtc accacgccag gatcttccat gtcgaggacg
      181 gccagcacgt cctcgtgggt ggctcgccca gcgaagctga ggatcatctc ctgcgaattg
      241 ctgtcggcat cgatgagaac gtcgttgccg aacagggtac cgctgatccg ggaggacctg
      301 agcccgttcg gataccggta ccgcagatct ccctccagat cccccagggt cagacggaaa
      361 tcgggcatcc ggagcccggc gtccaacatt ctgacgttga cgtccacctg cagctgctcg
      421 gaggcgtcca cgtcctcagg gagcttgatg ggaatgtgca ggttgccgag cacctcgagc
      481 cggccatccc cgacccactc cggcgccaca aatcccatgg actccgcaag gggcgtggtt
      541 cgcacgaagt ccagcgccag gtcggtctcc attcgagata cgagctccac gtccgcgtag
      601 gaggcgttgt tgcgcagcac gacctgggag ccgcccagcc ggacgccttc gcttgcgccc
      661 ttgtcgacgg tgacacgcac catcggaccg gcgaccacga cgtcggcgtc gagctcgtgg
      721 acgtccggcc agtctggcgc gtaggcaatg cggccatcgc tgatgcgggc tgacagctcg
      781 atccggcggg ccagctcgtt ccccttgagg tgctgctggc cgtgatagcc gaaccgaatg
      841 cgcgtcagca ggccggcttt cagcccgcca cgcagccagt cctcgagacc ctcgtcgatg
      901 gtgtacggca cgaacgtgaa cgcctgatcg gtgtccagag cgtccacgcc gatgagaaga
      961 gaaagccgct cctcgtagcg ctcgccgggc ggccgcgtga gggcaaagct gcccgccgcc
     1021 ctgctgaaca gggtctcggc ccgcaggttg acgcctcgaa ggccgagata accgttgccg
     1081 aaccaggcct gaaacgctcc ctgcaggtag ggcatctgcc aggtatccgt gaaggtctgg
     1141 ggaaacttga ggagcacgtc ctcgctcttc agcttcagct ccagcccccg ggcgtggccc
     1201 aaaagctctc ccgccgcccc gcgaacgaac ggggcaccgc ggtagccgtc aacgtccagg
     1261 ccggccagcg tggcggcaaa cccgctctct cccgtgggca gtcgatagta acctcggacg
     1321 ttgagcgctt cggcctgcag cttgagagcg ctcaaccagc ggcgcgcgcg ctccgaaccc
     1381 ctgagcgctc gctcgagaaa ctcggatacg ccacccatgt ccacccgttg agcccagaac
     1441 tgaaccactt ccgcatcttt gcggacgtgc acggcgggca tgcgcaatgc ccgctgcgtg
     1501 cgcacctgct cctcggaatc gtccagatgg gcaaccagcc caaaggccag gtcgtgcacc
     1561 acggcgtcgt gaacgtcttc gaagcctctg acgtccgcgc gtacgagccc ggccaactcg
     1621 acacccccgg gcagccgcag tcctcgcgcg tgggcgtgca gccgcccgcc ggattcgtca
     1681 ccgcgcaggt gccagtcgat tccgatactc atcaactcca gagggctgtc cagcacggca
     1741 gccggaatga caaagccgcc accgtccaac gaaagctgaa tccgctcgtc ccagaacggc
     1801 cagcggccgt cgcgggcctg gtactccagg tggagcccgc agtccgcggc gcagtcggcg
     1861 tcctggttgt cgagatgcag tcgatggcga tgcaggccac cgcggttcgt ggccaggtag
     1921 tccacctgca ccgactgtgc ctcttcctca ccggcaaggc caaaagcgat gacgccggag
     1981 aagcgcagct cgtcggcgtg atagacgatg ccgctcgcgt cgaactcgcc atcccccgat
     2041 gtccccgcca ggcgccattt cccggccggc gtcttctcga gccgcacggc accctccccg
     2101 acgagcaccc gccgcgccac gaagcggttg cggaccaggc tttccaacca gtcgagctcc
     2161 acgacgactt cccggagatg accggcgggg aggtccacgc gcgccgctcg aaccaccgga
     2221 ttgaggtagc gccacgaacc ttccagaccc gagacgcgaa cgcgctggcc ggacaaccac
     2281 tggttcgcga ccagttcgag atccggcagg aacatgaacc ccaaccgccc ggtgccctgc
     2341 atcagcgcga cgaagagcat cgtcagcgtg accacgacca ggaacggcct gagtacgatt
     2401 cggtgcaagt aatggggtcc tatgtgagat tagtttacgg aagtcggccc ggaatcggcc
     2461 tcaaggcccg gttcagccct tactaccttt cgaccccgca tttcacctta aatttccgtg
     2521 ccgttcgtcc ggtcagatca acattttcac tgcgtatcgc cgacgaggac gatatcgaac
     2581 tcgcccgcgc cgtagcttgg ttccaattcg atacgcaccc ggcgacccac gcgcgccgcc
     2641 atcgcggcca gccgatcggc gtcttcgtcc agtaacctgt ctacaacatt gtccgctgcc
     2701 cggatgacgt agtcgccctg cgagtgagcg ccgcacccac tttccgcatc ctgcatgatc
     2761 gagcggaaaa tctcgatgca cgtgctctgg gccgtcttca ccaccccggc gccgtgacag
     2821 tgcgagcacg gctcgcacaa ctgctgaacc aggctctccc tggtgcgctt ccggctcatc
     2881 tgcaccagcc cgagcgatga gaacccctcg atgcgaatcc gcgccgggtc gccctcgcag
     2941 gccttctcca gcgcgcgcag cacctgacgg cgatgctctt cgtcttccat gtcgatgaag
     3001 tccacgacga tgatgccgcc gaggttgcgc aggcgaagct gtcgcgggag cacctgggct
     3061 gcttccagat tcgtgcggaa aacggtttcc tctaggttgt gcttgccgag ataggcgccc
     3121 gtgttgacgt ccaccgtcgt catcgcttcc gtctgctcga tcaccaggtg accgccggat
     3181 ttgagttcca cgcggctgcc cagcgctcgg gtcagctcgt cgtcaacgcc gaagcgctcg
     3241 aaaatgggtc tcggctcgtc gtagagcact accttgtccg tcaactccgg caggaactcg
     3301 gcgacgaatt tccgcacccg ctccaacgtc ggcgggtgat cgattcgtat gcattccagc
     3361 tccgccgtcg ccatgtcgcg aatcacccgg gtgtgcagcg gcaactcctc gtaaacgatc
     3421 ccggggcagc cgcactcggc tcgcctggcc atgatccggt cccagatacg caggaggacg
     3481 cgcacgtcgg cttccagcgg ttcccggtcg acgccatccg cggcggtgcg cacgatgaag
     3541 cccacgccgg cgtcttgccc gccgcgcacc tcgtcgacga gcaagcgcaa ccgctcacgt
     3601 tcgtcttcct cgtcgactcg ctgggaaatc ccgatgtggt cgctgaaggg catgagcacc
     3661 agatagcgcg atgcgatggt gagctgggtg gtcaggcggg cacccttgct ggcgatggga
     3721 tctttcgcca cctggaccat cagctgctgg ccgtcgtgca gcagctcgcg aatgtcgacc
     3781 gccccgtccg gctcttcgcc gagcatcagc cgggggcctg cgatgtcgct gacgtgcagg
     3841 aagccggggc gttccaggcc acagtcgacg aacgcggcct gcatccccgg aatgatgcgc
     3901 tggacgcgac ccagatagat gttgccggtc aggctgtctg cgccggcccg ggcgacctgc
     3961 acctcctcga cgacggaccc cgacaccagc gcaacccggg actcgaagtc gttgacgttg
     4021 atgagcagtt cttcagacac ttgcccgcag ctcccacgtg ttcacgccaa tggcgtgaag
     4081 cagcgcttcg gtctccgcca ggggcaagcc gaccaccgcg ctgtagctgc cgttgatgct
     4141 ttgcgcaaat atacccccga tcccctgaat accgtaaccg cccgccttgt cgcgcggctc
     4201 gcccgtctcc cagtacgcgg cagcctcatc gcgggtgatg gcccgaaacg tcacgtcagc
     4261 ggccaccacg caggaccgca cggcccgccc gtcggtcacc gccacccccg tgagcacccg
     4321 atgggtgcgt ccggagaggc gcaggatcat gtcgatggcc gatgcccgat ccgccggctt
     4381 gccgaggatc tcctcatcca cgaccacggc ggtatcggcc ccgagggccg gcccgtcctc
     4441 tgccaccacc gcccgcgcct tgccgatcgc gaggcgctcc acgtatcgct cgggggactc
     4501 acccgggcga agcgactcgt ccacgtccgg cgggcggacg acgaagggaa ggccgatctg
     4561 acgaagcagc tcggcgcggc gcggcgaccc ggaagccagc acgatgtggc tcgccgaatg
     4621 gctcgccgaa tggcttgccg aatggctcgc cgaacggccg gctggcaccg acgccggact
     4681 cactcgacgc cgaattgcag ccgcaggcgc tgcagaaaca gggagacgaa cggccagacc
     4741 agcatgctcg tgagcgcggg gaccagcacg gcccagctcc agccgcgatc ggcggaaagg
     4801 tcgtgcacca gcatgcgcac cagatcaccg ccgaggatca gcaggaagac caccccgcac
     4861 tgctgaacca cggagtacat tcgcaggcgc tcgtagaagc gccagccgat gtaggtgatg
     4921 cccgccagca ccgcgccgtt gaggccgatg ggatccccga acagcacgtc gacgaagatc
     4981 ccgaagatcc acgcggatac cagccccagc cgatggggca gcgccatcac ccagaagaag
     5041 acgaacagcg gtacccaagc cggccggagc cagcccagcc actgcggcca cgtctccggc
     5101 agatgaaaga tggaaagcac cagggcaacc accagacttg cgaggatgag ccacccgccc
     5161 tgagcagggt tcacggcaac acctcgtcac gggtctcggg cgcgaacacc accagaacgt
     5221 gtcgcgatcg atccagcagc gccgacggcc gcaccagcac ccgggacagg ctcgccgccg
     5281 gatccaccac gaccgactcc acctccccca ccgggtaccc ctcgggaaaa cggcctccga
     5341 gtcccgaggt ttccaccaga tcacccttca ccacgtccgc cgtgatggtc acgttctcca
     5401 gctccagctg gtccatccgg cccgtgccgc cggcgatgct gcgaatgccg ttacggttga
     5461 tctgcacggg caccgcgtga tcccggtccg tgatgagcag cacccggctg gaatagacgt
     5521 ccacctccac cacctgaccg aacagccctt cggagtcgat gacggcctgg ccaatctgaa
     5581 cgccggcgtc cgagcccttg tcgatcacca cctgatgggc gcctaccgtc ggcacgatcc
     5641 cgatgatctc ggcaatgagc acctcgtccg ggagcctggc ccggctgccg agcagctccc
     5701 tgagattctc gttctccttg agaaggttcc ggtactgcag cgagatcttc gaaagctcca
     5761 gaacctgacg cttgagcatc tggttttcat cgagcagcgc ggagcgggtc gcgaacacgt
     5821 tgccgagctc ctctcccacc aggtacggcg tttccgccac gtagtgaagg ggagcgagca
     5881 tggtgccgaa cacctggcgg gtgccgcgca tgaaagaggt ggtgaggtcc agggtgacca
     5941 gtatcaggga tacgaggacg agcccggtga gcaggaagcc agcgccagat tctcgaacaa
     6001 acagcgcttt gatggcttga gtctccctat tcggtcgaca gcaggtccgc gttgccagcc
     6061 ttgtccatga gctcgagggc cttgccgccg ccgcgggcga cgcaggtcag cggatcctcg
     6121 gcgacgatca ccggcaagcc ggtctcctct gccaggaggg tgtcaaggtc acggagcagc
     6181 gcgccgccgc ccgtcagcac caggccggtt tccgcgatgt ccgaggcgag ctcgggcgga
     6241 cactgctcca gggcgctctt cagagcctgc acgatcatcg acaggggttc ctgcagcgct
     6301 tccaagatct cgttgctgtt cagcgtgaag ctgcgcggca ccccttccgc taagttgcgg
     6361 ccgcgaacgt cgatctcgcg cacctctttc tgcgggtagg cggccccaat ctcctgcttg
     6421 atgcgctccg ccgtggcttc gccgatgagg ctgccctggg tacggcgcac gtaggccacg
     6481 atggattcat cgaagcggtc tccaccgacc cgcacggtat ccgaatacac cacgccgttc
     6541 agcgagatga tggcgatctc cgtcgtgccg ccgccgatgt ccaccaccat ggtccccacc
     6601 gcctcctgca ccggcaggcc tgcgccgatg gcggccgcca tgggctcctc gagaagacgc
     6661 acgtcccgcg cgccggcgga aagcgcgctt tcgcggatgg cgcgccgctc gacctcggtg
     6721 gatttacagg gcacgcagac cagcacccgg ggactgggac ggatgaacga gttctcgtgg
     6781 accttggcaa tgaagtactt gagcatcttc tcggtgacga gaaagtcggc gatgacgccg
     6841 tccttcaggg gccggatggc ggtaatgttg cccggtgtgc gtcccagcat gcgcttcgca
     6901 tccagcccta ccgcggccac gctcttctgg ttgccctgca cccggatggc gacgacggaa
     6961 ggttcgttca gcacgatgcc ctgatccctg acgtagatga gcgtgttggc ggtgcccagg
     7021 tcgatggaca ggtcccggga gaacagcccg cgaatatttt taaacatctg gaataaggac
     7081 tcgggttaat tgactgaaca cttgatcccc tgacaagagg ggggatctcg gcggacggta
     7141 ctctatcaat ttgcaacggc aacgggaact gcccggcaca ccctcccggg caccggaaaa
     7201 tggcttcagt gaccgctttc cattacgctt cgcgccatgg ccagcatcga aatcgcccac
     7261 ctctgccgcc ttgcccagct ggcccttacg gagtcggaac aggccgccgc gctcgccgat
     7321 ctgcagcgca tcatcgccat gatagacgag atgcaggcca tggacaccga cggcgtcgag
     7381 ccgttggccc atccgatcga gtcggatcag cgtctgagac ccgacaccgt ctccgagacc
     7441 gtcgaccgcg atcactacca gcgcggcgcg ccggccaccc gggacggcct gtacctcgta
     7501 ccccgagtcg tcgagtaacg tggacccgtt tgcttctatc gggcagcacg cggcccggct
     7561 gcgagacggc agctactcga gcgttgagct cacccgtttc tttgtcgacc gcgcccggga
     7621 acatgccgac ctgaacgcct tcatctgcgt cgacgaggag ggcgcgctcg ccgcggccga
     7681 agctgccgat gcgcggctga gagcgggcac ggccacggcc ctcaccggca tccccctggc
     7741 gcacaaggac atcttctgca cccggggcac gatcacgtcc tgcgcctcga agatgctcga
     7801 gaacttcgta gcaccctacg acgcgacggt ggtggaacgc ctgaacgcgg ccggggcggt
     7861 caccgtcggc aaggccaaca tggacgagtt cgccatgggc tcgtccaacg agaacagccg
     7921 cttcgggccg gtgcgcaatc cctgggaccc aggccgagta ccgggcggat cctccggcgg
     7981 ttcggccgcc gcagtcgccg ccggcctcgt gcccgtggcc tccggcacgg acaccggcgg
     8041 ctccatccgt cagccgtccg ccttctgcgg cgtttccggc atcaagccca cctacggccg
     8101 cgtgtcccgc tacggcatgg tggcgttcgc gtcgagcctg gaccagggag gtttcctggg
     8161 cagaagcgcc gaggatcttg ccctgtccct ggccttctgc gccggcgcgg acccgcggga
     8221 ttccacctgc agcgagcacg ttgacgagtg gctcgacggg gtcgccgaga acggcgtcgg
     8281 aaatctggaa ggcggtctgc ggatcgggct gccgcgggaa tacttcgcgg atctttccac
     8341 gggctcggag ctgctggacg ctgtggccgg cttcttcgaa agcatcggct gctcgctcga
     8401 ggaagtctcg ctgccccata ccagcgcggc gattcccgcc tactacatca ttgccggcgc
     8461 ggaggcgtcc accaacctgt cgcgctacga cggcgtgcgt ttcggctatc gctgcgagaa
     8521 tccgggctca ctggaggacc tatacaggcg ctcgcgctcc gaggggttcg gcgcggaggt
     8581 gaagcgacgc atactcaccg gcacctatgc cctttccgtg ggctactacg acgcctacta
     8641 cgtgaaggct cagcggctgc ggcggctgat ccagagggac ttcctcgagg cgttcgagac
     8701 cgtggacatg atcctcgcgc ccaccgcccc cggcgtggcc ttcccgctcg gcgagctgac
     8761 ccacgatccc gtcgccatgt accagcagga catcttcacg ataccggcga gccttgccgg
     8821 cctgccggcc atgtccctgc cgtgcggttt tcaccagggg cttcccatcg gcgtgcagct
     8881 catcgggccg cacttcgcgg aaaagaccct cctgcaggcg ggcgcggcct atcagcgcga
     8941 gaccgactgg cacacccggc atccggaggc cggctgagat gggcttccgg attccccagg
     9001 gttgggaggc cgtcatcggg ctcgaggtgc acgtccagct ggccacgaga agcaagatat
     9061 tctccggggc ctcgacccgg ttcggcgcgg agccgaacac ccaggcctgc cctgtggatc
     9121 tgggcctgcc cggggtgctt cccgttctga acgcggaagc cgtgcgcatg gccgtgacgt
     9181 tcggcctcgc cgtcggggcg gagatccgac cctactcggt gttcgaccgg aagaactact
     9241 tctacccgga ccttcccaag ggctatcaga tcagccagct gcctcatccc atcgtcggcc
     9301 ggggcgcgat caccatcgaa gcctccggcg aagcgccccg gaccatcggc atcacgcggg
     9361 cccacctgga agaggacgcc ggtaagtcca ttcacgacgc gtacgccggt ttgaccggca
     9421 tcgatctgaa ccgggccggc accccgcttc tcgaggtggt ctcggaaccc gagctgcgca
     9481 cgccggagga ggcgggcgca tacttcagaa agctgcacag cctggtgcgc cacctgaaaa
     9541 tctgtgacgg caacctggcc gaaggcagca tgcgctgcga cgccaacgtc tcggtgcgcc
     9601 ccgccggcga gaccgctctg ggccagcgca cggagatcaa gaacgtcaac tctttccgtt
     9661 ttctcgagcg cgccatcgag ttcgagatcg agcggcagat cgacgttctc gaagccggcg
     9721 gcgccatcga gcagcagacg cttctctacg atccggacag ccacgagacc aggccaatgc
     9781 gcaccaagga atattccgag gactaccgct actttccgga cc
//
LOCUS       DBNHQR010000578         2805 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1449941, whole genome shotgun sequence.
ACCESSION   DBNHQR010000578 DBNHQR010000000
VERSION     DBNHQR010000578.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1449941"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..1750)
                     /locus_tag="AC16VR_13200"
     CDS             complement(125..1750)
                     /locus_tag="AC16VR_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HIR6012161.1"
                     /translation="MANLYENDLDANAANFAALTPLTFLARARDIYPHRTAVIYGKRR
                     YTWSEHYERCVRVSDALRRRGVGSGDTVAILAGNIPEMLEAHFAVPMAGAVLNAINVR
                     LDAPTVRFILEHGQAAVLLVDEAFGELASHALEGMASAPLVVGIEDSSLEDGRLIGAA
                     TWNELVAEGSSEATWALPGDEWAAIALNYTSGTTGNPKGVVYHHRGAYLNAVSNLLTW
                     NMGDHPTYLWTLPMFHCNGWCFPWTLAVNAGTSVCVRQVRADEIFDAIARYGVSHFCG
                     APVVLNTLLSADDGLKAKVTHQVNVMTAGAAPPAAVIEGMEAMGMSVTHVYGLTETYG
                     PVTLCAWQGERWDDLSPSERAALKARQGVRGHMLEGLMVADPETLVPVPMDGATLGEV
                     MMRGNNVMKGYLKNPDATAQSFEGGWFHSGDLAVWHPDGYIQIKDRSKDIIISGGENI
                     SSIEIEDTLYRHPKVREAAVVAKSDARWGETPCAFVELKPDVAASAEEIIDFCRENMA
                     RFKAPKHVVFGELPKTSTGKVQKFVLRERAEQI"
     gene            1858..>2805
                     /locus_tag="AC16VR_13205"
     CDS             1858..>2805
                     /locus_tag="AC16VR_13205"
                     /inference="COORDINATES: protein motif:HMM:NF013506.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HIR6012162.1"
                     /translation="MSEPGLMSRIGSWLTRARNFTANLIFVLLVVLLVTVILTSGERI
                     RVPEGAALVLNPTGIVVDKETLGDAIAELLAPESTRRETELAGLLTALETGAGDDRIK
                     TLVLDLTELDFVSTAHAATLGEAIRTFSDAGKEVIAYAHYFTQPQYLVASYADAVYMH
                     PDGQILPIGYEVNRLFFSEMLNKLDINVHIFRVGKYKEFVEPYTRSDMSDEAREANQK
                     LVDGLWRHYSETVTANREIEAQSFESYAEQYSPLLKAAAGDMGRLALEFHLVDELLTP
                     DQTRARIAATVGYAREGEFNGIGYRDYLTARDSDPARSGG"
BASE COUNT          519 a          945 c          847 g          494 t
ORIGIN      
        1 cggacccgcc ttgcgcgaat cgatcacgat gcccgacacg ccgggacctt cgcaaacctc
       61 caggctcagg gccctgcaca gctccggcca ggtccaaagc gggggcgatg cgtccacgcc
      121 cacctcagat ctgctccgcc cgttcccgca gcacgaattt ctggaccttg cccgtggacg
      181 tcttgggcag ctcgccaaac accacgtgct tgggggcctt gaaccgcgcc atgttctccc
      241 ggcagaaatc gatgatctcc tcggcgcttg ctgccacatc gggcttgagc tccacgaacg
      301 cgcacggcgt ctcgccccac ctggcgtcac tctttgccac caccgccgcc tcgcgcacct
      361 tcgggtggcg gtaaagcgtg tcctcaatct cgatgctgga gatgttctcg cccccggaga
      421 tgatgatgtc cttcgagcgg tctttgatct ggatgtagcc gtccgggtgc cagacggcca
      481 ggtcaccgga gtgaaaccaa cctccctcga aggattgagc ggtggcatcg gggttcttca
      541 ggtaaccctt catgacgttg ttgccccgca tcatcacctc gcccagggtc gcgccgtcca
      601 taggcaccgg caccagggtt tccggatctg ccaccatcag cccttccagc atgtgcccgc
      661 gcacgccctg ccgggctttc agggccgccc gctcggatgg ggacaggtcg tcccagcgct
      721 cgccctgcca ggcacaaagg gtgacgggcc cgtaggtctc ggtgaggccg tacacgtggg
      781 tcaccgacat gcccatggcc tccatgcctt cgattaccgc ggccggaggc gccgccccgg
      841 cggtcatgac attgacctgg tgcgtgacct tcgctttgag cccgtcatcg gcgctcaaca
      901 acgtgttcag caccaccggc gcgccgcaga agtgcgagac cccgtagcgg gcaatggcgt
      961 cgaatatctc atccgcgcgt acctgtctca cgcagaccga ggtgccggca ttgaccgcca
     1021 gggtccaggg gaaacaccag ccgttgcaat gaaacatcgg cagcgtccac agataggtcg
     1081 gatggtcccc catgttccac gtgagcaaat tggaaacggc gttcaggtat gcgccgcggt
     1141 ggtgatacac cacccctttg ggattgcccg tggtgccgga tgtgtagttg agggcgatgg
     1201 ccgcccactc gtcaccaggc agcgcccagg tcgcctcaga cgaaccctcg gccacgagct
     1261 cgttccacgt cgctgcacca atgagccggc cgtcctcgag gctcgagtct tcaatgccca
     1321 ccaccagggg cgcgctcgcc atgccctcca gcgcgtggga agccagctcg ccgaacgcct
     1381 cgtccaccag cagcacggcg gcctggccgt gctcgaggat gaagcgcacc gtcggcgcat
     1441 cgagcctgac gttgatggca ttgagcaccg cgcccgccat gggaaccgcg aagtgcgcct
     1501 ccagcatctc gggaatgttg cccgccagga tcgcgaccgt atcgccggag cccacgccac
     1561 gccgtctcag ggcgtctgac acccgcacgc agcgctcgta gtgctcggac caggtgtaac
     1621 gccgcttgcc gtagatgacg gccgtccggt ggggatagat atcgcgtgcc cgtgccagga
     1681 acgtcagcgg ggtgagcgcc gcaaagttgg ccgcattggc atccagatcg ttttcgtaga
     1741 gattggccat gtgcgtgggc gccggcggga aattctcact tcagcacagg gcaccgccta
     1801 gctcaagcac gtccagcatg agatgcttga aagatctaag caatccggag ccaccccatg
     1861 agcgaaccgg gcctgatgtc ccgcatcggc agctggttga cccgcgcgcg caacttcact
     1921 gccaacctga tattcgtgct cctcgtcgtt ctcttggtga cggtgatcct cacctccgga
     1981 gagcgcatcc gcgtaccgga aggggccgcc ctggtgctca atcccaccgg catcgtcgtg
     2041 gacaaggaga cgctgggcga tgcgattgcc gagctgctcg cacccgaatc gacgcgccgg
     2101 gaaacggagc tcgccggcct gctgaccgcc ctggaaacag gcgccgggga cgaccggatc
     2161 aaaaccctgg tgctggatct caccgagctc gacttcgtct ccacggccca cgccgcgacc
     2221 ctgggtgagg cgatcaggac gttcagcgac gctggcaagg aagtgatcgc ctacgcgcac
     2281 tacttcaccc agccccagta cctggtcgcc agctacgccg atgcggttta catgcatccc
     2341 gatggtcaga tactcccaat tggctatgaa gtaaaccgtc tgtttttcag tgagatgctg
     2401 aacaaacttg atataaacgt tcatatattc cgcgttggaa agtataaaga gttcgtggag
     2461 ccgtataccc gcagcgacat gtccgacgaa gcccgcgaag ccaaccagaa actggtggac
     2521 ggtttgtggc gccactactc ggaaacggtg accgccaacc gggagatcga ggctcagagc
     2581 ttcgagagct acgcggagca atacagcccc ctgctgaagg cggctgccgg tgacatgggg
     2641 cgactggcgc tggagttcca tctcgtcgat gagctcctca ctccggatca gacccgtgcc
     2701 cgtatcgcgg ccaccgtcgg ctatgcccgc gaaggcgagt tcaacggcat cgggtatcgg
     2761 gactatctga cggcccggga cagcgacccc gccagatccg gcggg
//
LOCUS       DBNHQR010000579         3521 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1513069, whole genome shotgun sequence.
ACCESSION   DBNHQR010000579 DBNHQR010000000
VERSION     DBNHQR010000579.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1513069"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="AC16VR_13210"
     CDS             <1..451
                     /locus_tag="AC16VR_13210"
                     /inference="COORDINATES: protein motif:HMM:NF015297.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HIR6012163.1"
                     /translation="CGSPRVRCLVMGTSDLVKELRGRHTRTRSNLNFALQRCVLSARS
                     AGVDILDGVHLDFRNAETFRDACEQGRDMGFDGKTLIHPTQIEAANEAFGYSAADVDH
                     ARAVIAAWESAQSEGKGVAVLDGQLVENLHAAEAQRVLAFAAAVGDR"
     gene            complement(485..1432)
                     /locus_tag="AC16VR_13215"
     CDS             complement(485..1432)
                     /locus_tag="AC16VR_13215"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR6012164.1"
                     /translation="MSISLGVHAGQQNMAMNDMRALWRKLDDKGVDWISAWDHFYEAP
                     PKGGTEPHFEALTTLGALAAETSNARIGCLVFYVGYRNPALLAKAATTLDHITSGRFE
                     LGIGAGWHVWEASAYGYPFPDIGTRLDMLEEAAQIIRGMLTQDRTSFSGKHYQVDDAS
                     CLPKPVQERLPIWIGGVGEKRTLKLVADYADGWNAAYLSPEEFARVNEVLNHWCEAEA
                     RDPASLRRAANVMFNLGMDARDLERQREALQRDWGAMADRVADGALLCTPEAAVDRIM
                     EYVNAGADEINIALRAPWNEEALDAYLTDIMPKVRALCE"
     gene            complement(1840..>3521)
                     /locus_tag="AC16VR_13220"
     CDS             complement(1840..>3521)
                     /locus_tag="AC16VR_13220"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6012165.1"
                     /translation="KAQRTLNELEDGDPTRLKSVTGWYARLLTITSRHALATLSGILV
                     ILVTIFWGYGAYGKGMVFFSDSDPQFAQVAVRARGNLSADEIDALVREVDSEILEVSG
                     IRTVNTYTLLSGGSTRGGFDRVGSMFLELHPESERDRKGAEIFAEIRQRTAGLAGIAV
                     EVQKLEQGPPVGKPVQIQFSSYDRELLEPAVTRVREYMETLPGLLDIDDTRSLPGIEW
                     KLSVDRAQAALYGADVSQVGIAVQLVTNGVKVGEYRPERADDAVDIRVRYPSDARGIS
                     ALDDLKISTTKGLIPISNFVKREPAPNVDTFQRIDGVPVEFIRANVAEGVLADDQVTL
                     IRDWLSAQTFDPDLDIDFRGANEEQAQSLAFVQVAFGLSLLLMFVLLVTQFNSIYQSA
                     LILVAVVMSTAGVLLGLLILQAPFSAILTGVGIVALAGIVVNNNIVLIDTYNHLRRQH
                     PDLDHVTLIVRTGAQRLRPVMLTTVTTIFGLLPLASNLSIDLVNRSIIYGGQLSGFWV
                     PLSQAIVSGLTFATLLTLIATPALLALPHQLKDLAAQLRALVSRTRQPTGA"
BASE COUNT          619 a         1070 c         1185 g          647 t
ORIGIN      
        1 ctgcgggtca ccgagggttc gttgtcttgt catggggacc agtgacctgg tcaaggaact
       61 gcgagggcgg cacacgcgaa cccggagcaa tctgaacttc gctctgcagc gttgcgtgct
      121 ctcggcccga tcggcagggg tcgacatcct cgacggggtg catctggatt tccgcaatgc
      181 ggaaacgttt cgtgatgcct gcgagcaggg ccgcgacatg ggatttgatg gcaagacgct
      241 gattcacccg acgcagatcg aggccgccaa cgaggccttc ggctactcgg cggcagacgt
      301 ggatcatgcc cgggcggtca ttgcggcatg ggaatccgcg caaagcgaag gcaaaggggt
      361 tgccgtgctc gacgggcagc tcgtcgagaa cctgcatgcg gcagaggcgc agcgggtgct
      421 cgcgttcgcg gccgcggtcg gcgatcgctg acgggcctat ccgatcagca gcagacgtcc
      481 gccgctattc gcagagggcg cggaccttgg gcatgatgtc cgtgaggtaa gcgtccagcg
      541 cctcctcgtt ccagggcgcg cgcaaagcga tgttgatctc gtccgcgccg gcattcacgt
      601 actccatgat ccggtccacg gcggcttccg gcgtgcagag cagggcgccg tccgccacac
      661 gatcggccat ggcgccccag tcgcgctgca gcgcttcccg ctggcgctcg aggtcccggg
      721 catccatgcc caggttgaac atcacgttgg ctgcgcgacg cagagaggcg ggatcccgcg
      781 cctcggcctc gcaccagtga ttgagaacct cgttgactct ggcaaactcc tccggtgaca
      841 ggtacgcggc gttccagccg tccgcgtagt cggcaacgag cttcagcgtc cgcttttcgc
      901 cgacgccgcc gatccagatc ggcaggcgtt cctggaccgg cttcggcagg cagctggcgt
      961 cgtcgacctg gtagtgcttg cctgagaagc tggtgcggtc ctgggtgagc atcccgcgga
     1021 tgatctgcgc agcttcctcg agcatgtcga gccgcgtgcc gatgtccgga aaagggtagc
     1081 cgtatgccga ggcttcccag acgtgccagc cggcgccgat gccgagctcg aaccgccctg
     1141 aggtgatgtg atccagagtg gtcgccgcct tggccagcag cgccggattg cggtacccga
     1201 cgtagaaaac gaggcagccg atgcgcgcgt tgcttgtttc ggcggccagc gcgccgaggg
     1261 tggtcagagc ctcgaagtgc ggctctgtgc cgcccttggg cggggcctcg tagaagtgat
     1321 cccacgcgga aatccaatcc acgcctttgt cgtcgagttt gcgccagagc gcgcgcatgt
     1381 cgttcatggc catgttttgc tggcccgcgt ggacgcccaa cgagatgctc atccctgcct
     1441 ctaccggttc agtgctgcgc cggcaggata catgagcggg agggcggacg gaattcgtcg
     1501 agttgccact gtcggggcgc tcgagctggt cggccgtcgc ttacgaactg atacagtctc
     1561 gaatcaagcg gttacaagat cgatgtggcg tttcggcggt tttatcggaa ctcttacgcc
     1621 gaatatcgga caaagtgtag tgagagctga gccatttgct ataaacgatc tcgaacggtt
     1681 tggagatgat atccagcgtt aagaaagaaa agatccacaa cgtctgacga gtttctgagt
     1741 accccggcgg caacctcccc tcccctcccc aatcccttgc cgccggggtc ttttttttgg
     1801 ccggcaaaga ccctcagggt gctgcaggac cggcgggttt caggcgccgg tgggctgccg
     1861 ggttcggctg acgagcgcgc gcagctgggc ggcgagatcc ttgagctgat gggggagtgc
     1921 cagcagcgcg ggcgttgcga tcagcgtcag cagcgttgcg aaggtcagtc cggaaacgat
     1981 ggcctgggat agaggcaccc agaagcccga cagctgaccg ccgtagatga tgctgcgatt
     2041 caccaggtcg atggacaggt tgctggcaag cggcagcagc ccgaagatcg tcgttaccgt
     2101 cgtcagcatg accgggcgca gccgctgagc gccggttcga acgatgagcg tcacgtggtc
     2161 caggtccgga tgctggcgcc ggaggtggtt gtacgtgtcg atgaggacga tgttgttgtt
     2221 cacgacgatg ccggccaggg ccacgatacc gacgccggtg aggatggcgc tgaagggtgc
     2281 ctgcaggatc aacagaccga gcagcacgcc ggcggtggac atgaccacgg cgaccaggat
     2341 cagcgcgctc tggtagatgc tgttgaactg ggttaccagc agcacgaaca tgagcagcag
     2401 ggacaggccg aaagcgacct gaacgaacgc cagggactgg gcctgctctt cgttggcacc
     2461 tcgaaagtcg atgtccaggt cggggtcgaa agtctgcgcg ctcagccagt cgcgaatcag
     2521 cgtcacctga tcgtcggcaa gaacgccttc ggctacgttg gcgcggatga actccaccgg
     2581 gacgccgtcg attcgctgaa acgtgtcgac gttgggggcg ggctcccgct tcacgaagtt
     2641 ggaaatgggg atcagccctt tggtcgtgct tatcttcagg tcgtccaggg cgctgatgcc
     2701 gcgagcgtcg ctcgggtacc tgacccggat gtccaccgca tcgtccgcgc gctccggacg
     2761 atactcgccg actttcacgc cgttcgtgac cagctgcacg gcaatgccga cctgggaaac
     2821 gtcagcgccg tacagggcgg cctgggcgcg atccacggac agtttccatt cgatgccagg
     2881 cagggatcgc gtgtcgtcga tgtccagcag cccgggcaag gtttccatgt actcgcgcac
     2941 cctcgtaacg gcgggttcca gcagctcacg gtcgtaggaa gaaaactgta tctgcaccgg
     3001 tttgcccacc ggagggccct gctcgagctt ctgcacctcg acggcaatgc cggccaggcc
     3061 ggcggtgcgt tgccgtatct ccgcgaagat ttccgcaccc ttgcggtcgc gctcgctctc
     3121 ggggtgcagc tcgaggaaca tggagcccac gcggtcgaaa ccgccccgag tggagccgcc
     3181 gctcaagagc gtgtaggtgt tgacggttcg tatgcccgaa acctcgagga tctcgctgtc
     3241 cacctcgcgg accagagcgt cgatttcatc ggctgacagg ttgccgcggg cgcgcacggc
     3301 aacctgggcg aactggggat cgctgtctga gaagaacacc atgcccttcc cgtaggcgcc
     3361 gtacccccag aagatggtga ccaggatgac caggatgccg gagagggtgg ccagggcgtg
     3421 tcggctggtg atggtcagca gccgcgcgta ccagccggtc acgctcttca gacgcgtggg
     3481 atcgccgtct tccagctcgt tgagcgtcct ctgcgctttg g
//
LOCUS       DBNHQR010000580         1488 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_817800, whole genome shotgun sequence.
ACCESSION   DBNHQR010000580 DBNHQR010000000
VERSION     DBNHQR010000580.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1488)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_817800"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="AC16VR_13225"
     CDS             complement(<1..860)
                     /locus_tag="AC16VR_13225"
                     /inference="COORDINATES: protein motif:HMM:NF023568.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6012166.1"
                     /translation="MKPGKLTVSDGAEIHYYRGLPGGEIRGAVQIAHGMGEHAGRYDA
                     LAGRLNQEGYAVYANDHRGHGLTAGADRLGIFGRGGWDRVIQDARELNDHVASKHPSA
                     PRALLGHSMGAMLTQQYIARHGDTIHAAVISGSPGLGSAFGLWLSQTMARLEHWRHGD
                     VVTERMQERVFAAPNASFEEPEATGFEWLSRDREEVRKYVEDPYCGFVVSAGSLSALL
                     GGAREARRRRTIGNIPAHLPVYVFSGSDDPVHGNERGLRNLLEGYRSRLHRVDYRLYP
                     EGRHEMLNEV"
     gene            complement(862..>1488)
                     /locus_tag="AC16VR_13230"
     CDS             complement(862..>1488)
                     /locus_tag="AC16VR_13230"
                     /inference="COORDINATES: protein motif:HMM:NF019887.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HIR6012167.1"
                     /translation="PAITCSGAQVYCPDIGGDVFDERLPADFTNDLYAICDAERCIAT
                     VTVDDRVLLKLDGKPEPGLFPEEMEHVAQLAGADPSLPRVAAIQGSHVNRLIREELQP
                     IYQDRVHFFDSIGPTGKVILTLTASTASKGDALRAACEHLGIDAAEVIAFGDSENDIE
                     MFRAAGASVAMGQADERTRAAAIVVSTPHDEDGVANAVEHLLRTGDIV"
BASE COUNT          235 a          478 c          506 g          269 t
ORIGIN      
        1 ttgacctcgt tgagcatctc gtgacgcccc tcggggtaga ggcggtagtc gacccggtgg
       61 aggcgggagc ggtacccctc gagcaggttg cgcagcccgc gctcgtttcc gtgaaccgga
      121 tcgtcgctgc ctgagaacac gtagacggga agatgggcgg ggatgttgcc gatggtgcgc
      181 ctgcgccggg cttcccgtgc gccccctagc agggcggaca aactcccggc actcaccacg
      241 aacccgcaat aggggtcctc cacatatttg cggacttcct cccggtctct ggacagccac
      301 tcgaaacccg tagcctcggg ctcctcgaac gacgcattgg gtgcggcgaa cacccgttcc
      361 tgcattcgct cggtgaccac gtcgccatgc cgccagtgct cgaggcgggc catggtttga
      421 gaaagccaca gaccgaaggc ggaaccgagc ccgggggagc cgctgatgac ggccgcgtgg
      481 atcgtgtcgc catggcgggc gatgtattgc tgggtgagca tggcgcccat gctgtgaccg
      541 agcagtgccc gaggcgcgct cggatgcttg gatgcgacgt ggtcgttgag ttccctggca
      601 tcctgaatga cccggtccca gccgccccga ccgaagatgc ccaaccggtc cgcacctgct
      661 gtgagcccgt ggccgcggtg gtcgttggca taaaccgcgt acccttcctg attgaggcgg
      721 ccggccagcg cgtcgtagcg gccggcatgc tcgcccatgc cgtgggcgat ctgaaccgcg
      781 cctcgaatct cgccgccggg aagcccgcgg tagtagtgga tctcggcgcc gtcgctgacg
      841 gtgagcttgc ccggcttcat gtcagacgat gtctcccgtg cgcagcaggt gttccacggc
      901 gttggcgacc ccgtcctcgt catggggggt gctgaccacg atggccgccg ctctggtgcg
      961 ctcgtcggcc tgtcccatgg cgacggacgc gccggcggcc cgaaacatct cgatgtcgtt
     1021 ctccgaatcg ccgaaggcga tcacctcggc agcatcgatt ccgaggtgtt cgcaggccgc
     1081 gcgcagggca tcgcccttgc tcgccgtgct cgccgtgagc gtgaggatca ccttgccggt
     1141 ggggccgatg ctgtcgaaga agtgcacgcg atcctgataa atcggctgta gctcctcgcg
     1201 aatcaggcgg ttcacatggg agccttgtat ggcggccacc cggggaagtg agggatccgc
     1261 tcctgccaac tgagcgacat gctccatttc ctcagggaag aggccgggtt cgggtttgcc
     1321 gtccagcttg agcagtaccc ggtcgtctac cgtgacggtg gcaatgcagc gctcggcgtc
     1381 gcagatcgcg taaagatcat tggtaaagtc ggcgggcagg cgctcgtcga acacgtcccc
     1441 gccaatgtcc gggcagtaga cctgggctcc ggagcaggtg atggccgg
//
LOCUS       DBNHQR010000581         5286 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1385384, whole genome shotgun sequence.
ACCESSION   DBNHQR010000581 DBNHQR010000000
VERSION     DBNHQR010000581.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5286)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5286
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1385384"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="AC16VR_13235"
     CDS             <1..384
                     /locus_tag="AC16VR_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016486038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR6012168.1"
                     /translation="YDTEAGEGGNRLSAGQKQLVSFARAILADPQILVMDEATSSVDT
                     HTEQRIQRGMANVLAGRIAIVIAHRLSTIRNADRIIVVEEGRITESGTHAELMGRRGH
                     YFELYKRQGLQESSATLSDLEPRPA"
     gene            complement(356..1756)
                     /locus_tag="AC16VR_13240"
     CDS             complement(356..1756)
                     /locus_tag="AC16VR_13240"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6012169.1"
                     /translation="MSKDYDVIVVGSGAAGLAAAVAAADAGASVLLVEADKRVGGSSR
                     LSGGHFYAAGTSTQREAGVDDDTADAMFEHYMTLNQWLVEPSVVRRYCDLSAPTFEWL
                     KGQGVSFSPQAVYRSGVGSVARGHPPEGSGEAVVNILDARRSSLGVDVVLDARVDGLL
                     LDDAGRVHGIRIGDDEATCGAVVMATGGFGANPEMLAEYYPAAAANGDWSWYIGTEMA
                     RGDGIRLGQDAGAAIDGHNRGLLLVTPGFSRDLEVLLPGWLVLINEQGRRFTDETAPY
                     TVLAGLIQHQTGKVYAVFDEAARRGAKRNPASQAYWVDEVLEQKADAGRILRADSLDE
                     LAAAAGIHADGFKGTVARYNADCEKGEDTAFFKSPETGMRAIEEGPFYAAEVRPAIIC
                     WTGTGLRIDAEARVLRTDEHPIPGLFAAGELVGSLHGDRYIGGGGSFGPCIVFGKLAG
                     ENAASYTLTQAWAQDR"
     gene            complement(1865..2854)
                     /locus_tag="AC16VR_13245"
     CDS             complement(1865..2854)
                     /locus_tag="AC16VR_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009045556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-thiouracil desulfurase family protein"
                     /protein_id="HIR6012170.1"
                     /translation="MADALPERPVPPLPIAISECLLGSKVRYDGSSSRSSFPHTLLDG
                     LFTYRGICPEVGIGMGTPREPIRLVGKPDDARVVGVKDPTMDVTERLIEFGRETAASL
                     TDVCGYVLMKNSPSCGLFRVKVYPVTDGEVTGAPDPRGRGAHAAAITAALPDLPAEEN
                     GRLHDPVLRENFVTRVFTYGHWQRCFGSADDLTAAKLVGFHSRYKYLLMAHSVPYYQA
                     AGRLLSDLRGDLPGKAAEYFSLLMRGLSRPATRKGHANVLSHLQGYFKRLLDGPSRQE
                     LEALIHSYRRGEQPLDAPLTLLKHHLRRHPQEYLEYQVYLDPHPGFAGLRRLL"
     gene            complement(2847..3797)
                     /locus_tag="AC16VR_13250"
     CDS             complement(2847..3797)
                     /locus_tag="AC16VR_13250"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6012171.1"
                     /translation="MSTRQMLTDSAERIFADHCTKALLDGAQEGEFPGDLWAVLKENG
                     FAELATADSGFELGDAFAVLKVAGRHAAPLPLAEIMLGNRWLGSADRLVSVGLVDGDV
                     VRDVPWGRRADAVIALDPSGGPPSVLEVTQVDSTGSNLAGEARDVLALTAALPLGSNE
                     PGLELLALARVAQMAGALERILELTLRYANEREQFGRPIARFQAIQHNLAVMAAEVAA
                     AVRIADAAVDALGGERFEFEVAAAKSRVGEAAGVCAESAHQIHGAMGFTHEHQLHHFT
                     RRVWAWREEFGNEIYWRRRLGDHIAGLGADAVWDFLATRG"
     gene            complement(3797..4942)
                     /locus_tag="AC16VR_13255"
     CDS             complement(3797..4942)
                     /locus_tag="AC16VR_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020296375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6012172.1"
                     /translation="MLQFDSMELPEETVALRSEVRSFLDSEHSHLTRRNSDFGSGHDP
                     EFSRKLGAKGWIGMAWPKAYGGGERTFFERYVVTEELLSAGAPVGAHWIADRQSGPLL
                     LRFGTEAQREKYIPPITRGESYFSIGMSEPNSGSDLASVRTTASKVADGWVVNGTKLW
                     STDAHRNNYMIALVRTEPPSENRHAGLSQVIIDLHDDGAQIRPIKNIAGHEDFNEVVF
                     EDVLIPEDRLVGEPGNGWEQVTSELAYERAGPERFLSSFRVFVELVRAVGKDPLPHEA
                     DAIGRIASHLMALRRMSISVAGMLEAGKSPQVEAALVKELGNNFEKLLPEIARLAAPD
                     GLSGDEERLRSFRSTFTDTLLGSPSFTIRGGTREILRGVIARGLGLR"
BASE COUNT          929 a         1819 c         1685 g          853 t
ORIGIN      
        1 tacgacacgg aagccggtga gggcgggaat cgtctttcgg cgggacagaa gcagctcgtc
       61 tccttcgccc gcgccatact ggcggacccg cagattctcg tcatggacga ggccacctca
      121 tcggtggata cgcacaccga gcagcggatt cagcggggca tggccaacgt gctggcggga
      181 cgcatcgcca tcgtcatcgc ccaccggctg tccaccatcc gcaacgctga ccgcatcatc
      241 gtggtcgagg agggccgcat caccgagtcg ggcacccacg ccgagctcat gggccgacgt
      301 ggccactatt tcgagctcta taagcggcag ggcctgcagg agtcgagcgc caccctcagc
      361 gatcttgagc ccaggcctgc gtgagcgtgt aagaggcggc gttctcacca gccagcttgc
      421 cgaacacgat gcaggggccg aaagaaccgc caccgccgat gtagcggtct ccgtgcagcg
      481 agcccaccag ctcgcctgcg gcgaagagcc cgggaatcgg gtgctcgtcg gtgcgcaaca
      541 cccgggcctc cgcgtcgatg cgcaggcccg tgcccgtcca gcagatgatg gccggacgaa
      601 cttccgcagc gtagaacggt ccctcctcga tggcgcgcat gccggtttcc gggcttttga
      661 agaacgccgt gtcctcgccc ttctcgcagt cggcgttgta gcgggccacg gtgcccttaa
      721 agccatccgc gtgaatgccc gcagcagcgg cgagctcgtc gagggagtcc gcccgaagga
      781 tgcgccccgc atcggccttc tgctctaaaa cctcgtccac ccagtacgcc tgggaggccg
      841 ggttgcgttt ggccccgcgc ctggcggctt cgtcgaacac cgcgtacacc tttcctgtct
      901 gatgctggat gagccccgca agcaccgtgt agggcgccgt ctcgtcggtg aagcggcgtc
      961 cctgctcgtt gatgaggacc agccatcccg ggagcagaac ttccagatcg cggctgaagc
     1021 cgggcgtgac gagcagcaga ccgcggttgt ggccgtcgat ggcggcaccg gcgtcctgac
     1081 cgagccggat gccgtcgccc cgggccatct cggttccgat gtaccacgac cagtcgccgt
     1141 tggccgccgc agccgggtag tactccgcga gcatctcagg gttggcaccg aaccccccgg
     1201 tggccatcac cacggccccg caggtcgcct cgtcgtcgcc gatcctgatg ccgtggaccc
     1261 gcccggcgtc atcgaggagc agcccgtcca ctctcgcgtc cagcaccacg tccacgccca
     1321 gactcgaacg acgggcgtcg aggatgttga cgacggcctc tccggatcct tccggcgggt
     1381 ggccacgggc aacgctgccc accccggacc ggtagacggc ctgggggctg aagctgacgc
     1441 cctgaccctt gagccactcg aacgtgggcg cggacagatc gcagtagcgg cggaccaccg
     1501 agggctcgac gagccactgg ttcagcgtca tgtagtgctc gaacatggca tcggccgtgt
     1561 cgtcgtcgac cccggcctcc cgctgggtgc tggtgcccgc cgcatagaaa tgcccgccgc
     1621 tgagacgtga cgatccgccc acccgtttgt ccgcctcgac cagaagcacg ctggctccgg
     1681 cgtctgcagc agcgacggcc gcggcgagac cggctgcgcc gctccccacg acgatcacat
     1741 cgtagtcttt gctcatggtg tccctcccct ggctgcgttc accatcgcgt cgggaagagt
     1801 ttaccccgtt aagggcgacg cgccaccggt tggtcaacgg caagggcccc agggcgcaga
     1861 acgctcagag caatcgacgc aggccggcaa agcccggatg aggatccaga tagacctgat
     1921 actccagata ctcctgaggg tgacggcgca gatgatgctt caacagggtg agcggagcgt
     1981 ccagcggctg ctcgccacgg cggtagctgt gaatcagcgc ttcgagctcc tggcggctgg
     2041 gcccgtccag aaggcgcttg aaatatccct gcagatggga gagcacgttg gcgtgccctt
     2101 tgcgggtcgc cggtcgggac aggccccgca tgagcaggga gaaatactcg gccgccttgc
     2161 cgggcagatc gccgcgcaga tcgctcagga gcctgcccgc cgcctgatag tacgggacgc
     2221 tgtgcgccat gagcaggtac ttgtaccggc tgtgaaagcc gacgagcttt gcggcggtga
     2281 gatcgtcagc cgagccgaaa cagcgttgcc agtgcccgta ggtgaaaacc cgggtcacga
     2341 agttttcccg cagcactgga tcgtgcagcc tgccgttctc ctccgccggc aggtcgggca
     2401 gcgcggcggt aatcgccgca gcgtgagcgc cgcggccccg gggatcgggc gcgcccgtca
     2461 cctcgccgtc ggtgacaggg taaaccttga ccctgaacag gccgcagctg ggcgagttct
     2521 tcatgagaac gtaaccgcag acgtccgtca gcgacgccgc cgtctcccga ccgaattcaa
     2581 tgagccgctc ggtgacgtcc atcgtcgggt ctttcacccc cacgacccgc gcatcgtccg
     2641 gcttgccaac gagcctgata ggctcccgcg gcgtgcccat gccgatgccc acctccggac
     2701 agattccccg atacgtgaac agaccgtcga gcagcgtgtg cgggaacgac gatcgtgacg
     2761 agctgccgtc gtagcgtacc ttgctgccca ggaggcactc gctgatggca atgggcaggg
     2821 gcgggaccgg ccgctctggc agggcgtcag ccacgcgtgg cgaggaagtc ccacacggca
     2881 tcggcaccca gcccggcgat gtggtctccc aggcgacggc gccagtagat ctcgttgccg
     2941 aactcctcgc gccaggccca gacgcggcgg gtgaaatggt gcagctgatg ctcgtgggtg
     3001 aagcccatgg cgccgtgaat ctgatgggcg ctttccgcgc aaacgccggc agcctcgccg
     3061 accctggact tggcggcggc aacctcgaac tcgaaacgct caccgcccag ggcatccacg
     3121 gcggcgtcgg cgatgcgcac cgcggcggct acctcggccg ccatgacagc cagattgtgc
     3181 tgaatggcct gaaagcgcgc gatggggcga ccgaactgct cccgttcgtt ggcataccgc
     3241 agggtaagct cgagtatccg ttccagcgcc cccgccatct gggccacgcg ggcaagggcc
     3301 aggagctcga ggccgggttc gttgctgcca agcggcaagg ctgcggtcag ggcgagcacg
     3361 tcccgagctt cgccggccag gttgctgccc gtagagtcga cctgcgtcac ctccagcacg
     3421 gacgggggtc cgcccgaggg gtccagcgcg atcacggcgt ccgcccggcg gccccagggc
     3481 acgtctctca ccacgtcccc gtccacgagc cccaccgaca ccagacgatc ggcactgccc
     3541 agccaccgat tgcccagcat gatctcggcc agcggcagcg gcgcggcgtg acggcccgcc
     3601 accttgagca cggcgaaagc atctccgagc tcgaagccgc tgtctgccgt cgcaagctcg
     3661 gcaaagccgt tctccttgag cacggcccag aggtcgccgg gaaactctcc ttcctgtgcc
     3721 ccgtccagca gggccttggt gcagtgatcg gcgaagatcc tttccgcgct gtccgtgagc
     3781 atctgacgcg tgctcatcag cgcagcccca gcccgcgggc aatcacgccc cgaagaattt
     3841 cccgcgtccc gccgcggatg gtgaacgagg gcgacccgag cagcgtatcc gtaaacgtcg
     3901 agcggaacga acgcagccgc tcttcgtcgc cgctcagacc gtccggagcc gcgagccgcg
     3961 cgatctccgg aagaagcttc tcgaagttgt tgcccagctc cttgaccaaa gcagcttcca
     4021 cctgggggct cttgcccgct tcgagcatgc cggccacgga gatggacatg cgccgcagcg
     4081 ccatcagatg cgaagcgatg cggccaatgg cgtcagcttc gtgaggaagc ggatctttac
     4141 ccacggccct gaccagctcc acgaatacgc ggaaactcga caggaaccgt tccggacccg
     4201 cccgctcgta ggcgagctcg ctcgtgacct gctcccagcc gttgccgggc tcgccgacca
     4261 gacggtcctc aggaatcaga acgtcttcga agaccacctc gttgaagtcc tcgtgcccgg
     4321 cgatgttctt gatcggacgg atctgcgccc cgtcgtcgtg cagatcgatg atcacctggg
     4381 agagaccggc atgacggttc tcagagggag gctcggtgcg aaccagcgcg atcatgtagt
     4441 tgttgcgatg cgcgtccgtg gaccacagct tcgtcccgtt gacgacccaa ccatccgcga
     4501 ccttgctggc cgtggtccgc acggaggcga ggtcagatcc cgagttcggc tcgctcatgc
     4561 cgatggagaa gtagctttcg ccccgggtga tcggcgggat gtacttctca cgctgggcct
     4621 cggttccgaa gcgcagcagc agcggtccgc tctgacggtc cgcgatccag tgcgcgccca
     4681 ccggggcgcc cgcgctcaag agttcctcgg tgaccacgta gcgctcgaaa aacgttctct
     4741 ccccgccgcc gtaggccttg ggccaggcca tgccgatcca acccttcgca cccagcttcc
     4801 gggaaaattc agggtcgtga ccggaaccga aatccgagtt tcggcgcgta aggtgcgaat
     4861 gctcgctgtc gagaaacgag cgcacctcgc tgcgcagcgc caccgtttcc tcgggcagct
     4921 ccatggagtc gaattgcaac accgggaatc gtccaatgca gataaggcgt cgctaaatta
     4981 ctgacagccc aggttcattg caaaccgctc gcctcgttgt cgggcaattt cgcgttgcct
     5041 acactagcga catcgattcc gcagctgaaa aatccgatga cgtcgatccc caaacgagag
     5101 cagagctcga atagacctac cccgggcgaa cgcgcgctca accggcggcg accgccggca
     5161 gcatgaggaa agaggatctg caggccgcct tcggaccggt gctcgatgag gtcgccgagc
     5221 gttcccaggt agccgacgct ttcctggaca aggatctcta caggatctac atcgccacgc
     5281 tatggg
//
LOCUS       DBNHQR010000582         4618 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_58336, whole genome shotgun sequence.
ACCESSION   DBNHQR010000582 DBNHQR010000000
VERSION     DBNHQR010000582.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4618)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_58336"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..1269
                     /locus_tag="AC16VR_13260"
     CDS             205..1269
                     /locus_tag="AC16VR_13260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR6012173.1"
                     /translation="MKAETLLPLGKLDPGLAAPEAALDLHDVAEDARTVDALGYWGLL
                     MEETKDDPFQVLAMAAAATSRVRLGTSVAIAFARSPFVTAMSAWTLQKLSNGRFELGL
                     GSQVRGHIRRRFGLQWHPPGPWMRDYVRAVRAIWASWQNGTKLDFSSQHYEIDLTVPL
                     FTPTPIDHPDIPIFVAAVNAYMCGVAAEVADGVRLHPVCSPRYIRDVVAPAVAEKRPA
                     GKPFEVCLKPLIAAADDGKTLSERAEVARQRLAFYVSTPAYRRPFENAGLGDLAAELS
                     RLSKEQRWHAMAERISDDVLEEWAVVATYDELAATIRSRFDGLVDRVEVGIPVASPED
                     RESLSRIVAELQSGGGSRAS"
     gene            1419..2405
                     /locus_tag="AC16VR_13265"
     CDS             1419..2405
                     /locus_tag="AC16VR_13265"
                     /inference="COORDINATES: protein motif:HMM:NF016241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HIR6012174.1"
                     /translation="MLTGILSSHAHATDFVMMKNGDRITGEIKQIWNGEVFIEPEYGD
                     TYPIDLDYVAYVHTDEDFEVEFVEGRRSRTVTGRLGLDDLGRPSVIVGDAAETYPLAK
                     IDNMQEIEDFFEWELATDISVNLATGNSDTRSSRFNVVGKMKLGEHRHRLEYTRDQAS
                     AEGDTTKDQTYVAYEDVWTFRDDWFVRGAVNWTRDPIRDLASRSQIFLGPGFHFWDDS
                     KRTLNLSVGPNYLSEDIGGENKQSVAAQLVFRYEQHFLDDDLVVFQETDYQTIVSGRK
                     NKILNTSTGVRWDVLEDVYIKMQVDFDYETNPASAREKEDVTYLVGVGLELD"
     gene            complement(2422..3294)
                     /locus_tag="AC16VR_13270"
     CDS             complement(2422..3294)
                     /locus_tag="AC16VR_13270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6012175.1"
                     /translation="MKVGLAFASSIGIDGATALDICRRAEAAGFESVWGGEHVIIPDA
                     IASKYPYTPDGKVPVEADTPIPDPLMWLAFAAAAAPTLRLGTCILIVPQRNPLILAKE
                     LATLDQLSGGRVELGLGVGWLKEEFEALGVPWERRGARNDEYIAAMRALWAGPHAEYH
                     GDFVDFAPVTCSPRPVNGSIPVIVGGDTDAAIARAVRLADGYFPGEGDAERLGALLGR
                     LRAAAEQAGRDPASIEVNAMFGVQMADPAAGIEQMAGLGVDRIMIPAFFFAGPDGLDR
                     LSEFGERVMPLTRG"
     gene            3456..3752
                     /locus_tag="AC16VR_13275"
     CDS             3456..3752
                     /locus_tag="AC16VR_13275"
                     /inference="COORDINATES: protein motif:HMM:NF012267.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HIR6012176.1"
                     /translation="MTEACIGVKGEGCMEVCPEFCILSEPADHMSYIDPARCTDCGVC
                     VAACVVGAIFADKDVPRASREFININESWFRHKTGVRRRVRAMASEIGHWLPPE"
     gene            complement(3801..4565)
                     /locus_tag="AC16VR_13280"
     CDS             complement(3801..4565)
                     /locus_tag="AC16VR_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012177.1"
                     /translation="MRNKILYTLGFALFVLVWLGVMDARADSHAAVAPSVLESYACSY
                     KPGKDREDLDKLRDFYLKQAEKAGIAAPPAWLWTLNKGAAPVNFVWLNVHESLTAFGG
                     TDDAIAASPEMAEAFERADTVMTCQAGLGTFRQTYGGEMQESDSSVVSFGCGFREGSG
                     PATMDALHAQIADLNEMAGDLAVNSIFEVTPLTQGPGGSDIVMVVITDGTSGWARHMD
                     YINSIPAGLAAQSHFNAALDCNMNLWRLEQIVGGGD"
BASE COUNT          903 a         1532 c         1408 g          775 t
ORIGIN      
        1 gaagtctaca aaagcgggtg gcgaggttgt tcagactatt gaggtaagag caaacccgcc
       61 agatgggggg agatcacggg cgggcgcgta ttaccgccgg cgccaggcgg gaaggatcag
      121 ccaatcaggg cgtttcgacg accccccacc ggttcgttag actctccccg ccttcaccac
      181 gtcgaccggg acggggaaac cgacatgaaa gccgagaccc ttctgccgct gggcaagctg
      241 gatccagggc tcgcggcgcc cgaggcagcg ctcgacctcc acgacgttgc agaagatgcc
      301 cgcaccgtgg acgcgctcgg ctactggggg cttctcatgg aggaaaccaa ggacgatccg
      361 ttccaggtgc tcgccatggc ggccgcggca accagccgcg tgcgcctggg cacctccgtg
      421 gccatcgcct tcgcccgcag tccattcgtc accgccatgt cggcgtggac attgcagaaa
      481 ctgtccaacg gccgcttcga gctcggcctg ggttctcagg tccgcggcca catccgcaga
      541 cgcttcggcc tgcagtggca tcctccggga ccgtggatgc gcgactacgt gagggcggtg
      601 cgagccatct gggcgagctg gcagaacggc acgaagctcg acttttcgag ccaacattac
      661 gaaatcgacc tgaccgtacc cctttttacg ccgaccccca tcgatcatcc cgacattccc
      721 atattcgttg ccgcagtcaa cgcctacatg tgtggcgtcg ccgcggaggt ggccgacggg
      781 gtgcgcctgc atcccgtctg ctcgccccgc tacatccggg acgtggtggc acccgccgtg
      841 gccgagaaac ggcccgcggg caagccgttc gaagtctgcc tgaagccgct gatcgcggcc
      901 gccgacgacg ggaagacgct cagcgagcgg gcagaggtcg cccggcagcg cctggccttt
      961 tacgtgagca cacccgcata ccgccgtccg ttcgagaacg ccggcctggg cgacctcgct
     1021 gccgagctca gccgcctgtc taaggaacag cgatggcatg cgatggccga gcgcataagc
     1081 gacgacgtgc tggaggagtg ggcggtggtc gccacctacg acgagctcgc cgcaacgatt
     1141 cggtcccgct tcgacggtct cgttgacagg gtggaggtcg gcatcccggt cgcaagcccc
     1201 gaggacaggg agtcgctgtc gaggatcgtc gccgagttgc aaagcggtgg cgggagccgc
     1261 gcgagctgag ccggggctcg tccaccgcac gagaattgga cctttccagc gaattgtggc
     1321 agaacacccg cccctgaaga gcgcgccact catcgcgccg ctgaattcgt cgagctcgag
     1381 gacccgacat gcgacatttc ccattgctca tcgcagcaat gcttaccggc attctgagca
     1441 gccatgccca cgcgaccgac ttcgtcatga tgaagaacgg cgaccgaatc acgggcgaga
     1501 taaaacagat ctggaacggc gaggtcttca tcgagcccga gtacggcgat acgtatccca
     1561 tcgacctcga ctacgtcgct tacgtgcaca cggacgaaga cttcgaggtc gagttcgtcg
     1621 agggccggcg cagccgcacg gtgacgggac ggctggggct ggacgatttg ggccgtccgt
     1681 ccgtcatcgt cggcgatgcg gcggagacct acccgctggc caagatagac aacatgcagg
     1741 aaatcgagga cttcttcgaa tgggaattgg cgaccgacat cagcgtcaac ctcgcaaccg
     1801 gaaactcgga cacccgctcc tcccgcttca acgtggtggg aaaaatgaag ctgggggagc
     1861 atcgacaccg gctcgaatac acccgggacc aggcgagcgc cgaaggcgac actaccaagg
     1921 accagacata cgttgcctat gaggacgtct ggacgttccg cgacgactgg ttcgtccgcg
     1981 gcgccgtcaa ctggacgcgc gacccgattc gggacctggc gagccgaagc cagatcttcc
     2041 tcggccccgg cttccacttc tgggacgact ccaagcgcac gctgaacctc agcgtcggcc
     2101 ccaactacct ctcggaggac atcggcgggg agaacaaaca atccgtggca gctcagctgg
     2161 tcttccgcta cgagcagcac ttcctggatg acgatctcgt cgtttttcag gaaaccgact
     2221 accagaccat cgtgagcggc cggaagaaca agattctcaa caccagcacg ggcgtgcgat
     2281 gggacgtgct cgaagacgtc tacatcaaga tgcaggtgga cttcgactac gagaccaacc
     2341 cggcgagcgc ccgcgagaaa gaagacgtca cctatctggt cggcgtcggg ctggagctcg
     2401 attgagtccg ctgaggcgcg ctcaaccccg ggtcaggggc atcacccgct cgccgaactc
     2461 gctgagacgg tcaagaccat ccggtccggc aaagaagaag gccggaatca tgattctgtc
     2521 cacgcccagc cccgccatct gctcgatgcc ggccgccggg tctgccatct gcacgccgaa
     2581 catggcgttg acctcgatgc tggcggggtc ccgacccgcc tgctctgccg cggcacgcag
     2641 ccgtcccagc aaggcaccga gccgctccgc gtccccctcg ccagggaaat agccgtccgc
     2701 caggcgcacc gcgcgagcga tggccgcatc ggtgtcgccg ccgacgatca ccggaatcga
     2761 gccgttcacg ggacgcgggc tgcaggtcac gggcgcgaaa tccacgaaat ccccgtgata
     2821 ctccgcatgt ggtccggccc aaagcgcgcg catggccgcg atgtactcgt cgttgcgcgc
     2881 tccgcgccgc tcccagggca cgcccagggc ttcgaactcc tccttcagcc acccgacgcc
     2941 gagaccaagc tcgacccggc cgccggagag ctggtccagc gtcgccagct ccttcgcgag
     3001 aatcaggggg ttgcgctggg gcacgatcag aatgcaggtg ccgagacgca acgtgggcgc
     3061 cgccgcggct gcaaaagcca gccacatgag ggggtcgggg atcggggtat ccgcctccac
     3121 cggaaccttg ccgtccggcg tgtagggata tttggaagcg atggcatcgg ggatgatgac
     3181 gtgctcaccg ccccaaaccg actcgaatcc cgccgcctcg gctcggcggc agatatccag
     3241 agcagtagcg ccatcgatgc cgatgctgct cgcgaaggct agtccgactt tcaccgtttg
     3301 attcccctgc tggtttgaaa ggcacatcct acaccgcctc gttcgccagg acccaatctc
     3361 ttccgggtcc cgatcggaaa cccgcagggt agccccgggc ttggggtaag atcggctgac
     3421 cgcagacgaa cggaacagaa ttgatgaccc acgtcgtgac tgaagcctgc atcggcgtca
     3481 agggcgaggg gtgcatggag gtgtgcccgg agttctgcat cctcagcgag ccggccgatc
     3541 acatgtcgta catcgatccc gcccgctgca cggactgcgg ggtctgcgtc gccgcctgcg
     3601 tggtcggcgc catattcgcc gacaaggacg ttcccagagc atcgcgggag ttcatcaaca
     3661 tcaacgaatc ctggtttcgc cacaagacgg gcgtgcgcag gcgcgtcaga gcaatggctt
     3721 ccgaaattgg ccactggctg ccgccggaat gaggcgctcc gcgaggagcc gacccgcatg
     3781 gggtcggccc cgcgtcgaga ttaatctccg ccgccgacga tctgctcgag acgccagagg
     3841 ttcatgttgc aatccagagc ggcattgaag tggctctggg ctgcgagccc ggcaggtatg
     3901 gagttgatgt agtccatgtg acgggcccat ccggacgtgc cgtccgtgat taccaccatc
     3961 acgatgtcgg acccgcccgg tccctgcgtc agcggcgtga cttcaaagat cgagttgacc
     4021 gccagatcgc cggccatctc attcagatca gcgatctggg cgtgaagggc atccatggtc
     4081 gccggaccgg atccctctct gaatccgcac ccgaatgaaa caacgctgct gtcactttcc
     4141 tgcatctctc caccatacgt ctgcctgaac gttcccagtc cagcttgaca ggtcatgacg
     4201 gtatctgccc gctcgaaagc ttccgccatc tccggcgatg cggcgatggc atcatcggtc
     4261 cccccgaaag cggtaaggct ttcatgcacg ttcaaccaaa cgaagtttac cggtgcagcg
     4321 cccttgttga gggtccacaa ccatgcaggc ggcgcggcaa tgccggcctt ttccgcctgc
     4381 ttgagataga agtccctcag cttgtccaga tcttccctgt ctttccctgg cttgtagctg
     4441 caggcatagc tttccagaac actcggcgcc accgccgcat gggagtcagc ccgagcatcc
     4501 atcactccca gccagaccaa cacgaacaaa gcaaatccca acgtgtagag aattttgttc
     4561 ctcatagtca actgaacctc ctgtgcacct cgaccatcca gccccggcta cagtctcc
//
LOCUS       DBNHQR010000583         2038 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_374359, whole genome shotgun sequence.
ACCESSION   DBNHQR010000583 DBNHQR010000000
VERSION     DBNHQR010000583.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_374359"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="AC16VR_13285"
     CDS             <1..656
                     /locus_tag="AC16VR_13285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6012178.1"
                     /translation="LCPTSHPIGTKRICVDTGSYETYNRPNVTLVDISGTPIDTVTPK
                     GLRVRNVDYEFDVLVLATGFDAMTGALLGMDIRGVGGERLADHWRAGPRSYLGLCVTG
                     FPNLVTITGPGSPSVLSNMMVSIEQHVDWVADCLGHMADRGLTRLEALQQAEDGWVDH
                     VNEVANATLFPKGGSWYLGANIPGKPRVFMPYAGGVGPYRDICDGVAARGYEGFSIT"
     gene            746..1906
                     /locus_tag="AC16VR_13290"
     CDS             746..1906
                     /locus_tag="AC16VR_13290"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIR6012179.1"
                     /translation="MAESKRDTSADYGPHEEAMGAYLREGEARALALGNRGPIRFGAD
                     GRLAADILDAYSRLGFYVFTGVLSAEELDDLRSDVEDMWSRLPVSRGAAVDALGRPAL
                     GADLRAPTVQWARPLSDPLGGTDKANGRHPAKMFEPQPPADAPPFVCQLVLGSLQFSD
                     ACLRVYGHPQLLAVAAAINGDDFAPFNEAVWRKEPGLGPSVAWHQDGTTHWDSPDLDE
                     GTHGFNFMAQIYGCTAANAVWVVPGSHARGKVDIAALVARAGSDRLPDAVPLICDPGD
                     VAICNRQAVHGSFANTGGDLRVTVNFGFHRRQSLLGVEGGGIHNRREIYDERRIRERS
                     RVIGYAIDARRQRFPDEAPYVYRPFAGEEDEYRWSDAARARLKDYNLLDLGI"
BASE COUNT          317 a          675 c          689 g          357 t
ORIGIN      
        1 gcctgtgccc cacgtcgcat cccatcggaa ccaaacggat ctgcgtggac accggctctt
       61 acgagaccta caaccgcccc aacgtgacgc tggtggacat ctccggtacc cccatcgata
      121 ccgtcacgcc gaaagggttg cgggtgcgca acgtcgacta cgagttcgac gttctggtgc
      181 tcgccacggg cttcgacgcc atgaccggcg cgctgctcgg catggacatc cggggtgtcg
      241 ggggggagcg gctggccgat cactggcgtg cgggcccgcg gagctacctg ggcctgtgcg
      301 tcaccgggtt tcccaacctg gtgaccatca ccggcccggg cagcccctct gttctgagca
      361 acatgatggt ttccatcgag cagcacgtgg actgggtggc cgactgcctg ggccacatgg
      421 ccgatcgcgg gctcacccgc ctggaggctc tgcagcaggc tgaggacggt tgggtggatc
      481 acgtcaacga ggtcgccaac gccacgctgt tccccaaggg agggtcctgg tatctcggcg
      541 caaacattcc cggcaagccg cgcgtgttca tgccctatgc gggcggcgtg ggcccgtatc
      601 gtgatatctg cgatggcgtg gccgcgcgcg gctacgaggg cttttcgatc acctagcggc
      661 gccgcagcgc gggtcgacgg caaggcttgc ccgaggcgat acttcggggg cgcgcaccgc
      721 gatgcggatc gatcgggaga taggtatggc tgagtcgaag cgggacacca gcgccgacta
      781 cgggccccat gaggaggcca tgggcgccta tcttcgcgaa ggcgaggcgc gcgcccttgc
      841 cctgggtaac cggggcccca tccgcttcgg cgccgatggt cggctcgcgg cggacatcct
      901 cgacgcctac tcgcgcctgg gcttctacgt gttcaccggg gtgttgtcgg ccgaggagct
      961 ggacgatctc cgttcggacg tcgaggacat gtggtcgcga ctgccggtct cccggggtgc
     1021 tgcggtggac gcgctgggtc gtcccgcgct gggcgccgac cttcgcgcac ccacggtgca
     1081 gtgggcgcgg cccctcagcg atcccctggg cggcacggac aaagccaacg gtcggcaccc
     1141 ggccaagatg ttcgagccgc agccgccggc cgatgcgccg cctttcgtct gccagctggt
     1201 gctcgggtcc ctgcagttct ccgatgcgtg cctcagggtt tacggccatc ctcagctgct
     1261 cgcggtggcg gcagccatca acggggacga tttcgcgccg ttcaacgaag ccgtctggcg
     1321 gaaggagccg ggtctgggcc cgtcggtcgc ctggcaccag gacggtacca cccactggga
     1381 cagtcccgat ctcgacgagg gaacccacgg attcaatttc atggcgcaga tttacgggtg
     1441 cacggcggcg aacgccgtct gggtcgtgcc gggatcacac gctcgcggaa aagttgacat
     1501 cgccgcgctc gtcgcccgcg caggctcgga tcgccttccc gacgcggtgc ccctcatctg
     1561 cgatcccgga gatgtggcca tctgcaaccg tcaggcggta cacggatcct tcgccaatac
     1621 cgggggcgat ctgagggtca ccgtcaactt cggatttcac cgacgccaat cgctgcttgg
     1681 cgtggagggc ggcggcatcc acaaccgccg ggagatatac gatgaacggc gtatccgcga
     1741 gcggtcgcgt gtgatcggtt acgccatcga tgcgcgcagg cagcgctttc ccgacgaggc
     1801 gccctacgtc tatcgaccgt tcgcaggcga ggaggatgag taccgctgga gcgacgcggc
     1861 ccgcgcgcgg ctcaaggact acaacctcct cgacctgggg atctaaggcg cctaggactc
     1921 ttcggcgttc atgacctcgt agatgcgctg ctggaagtac cagcgcccgt ccaccttgac
     1981 gtagtcgtcc tcgtaacgtc ccgtgacgtt gcgtttcacg ccgtccttct ggtggaga
//
LOCUS       DBNHQR010000584         3346 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_754199, whole genome shotgun sequence.
ACCESSION   DBNHQR010000584 DBNHQR010000000
VERSION     DBNHQR010000584.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_754199"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1228
                     /locus_tag="AC16VR_13295"
     CDS             <1..1228
                     /locus_tag="AC16VR_13295"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6012180.1"
                     /translation="RDNMPRVLYSNANLLDGEHAARPGSSVIVEDGHILAVGTPESMA
                     AAAYDELIDLEGRTLMPGLVMGHYHAAYRGYGEEGGSQTATAVEQAFFALGNVQIALK
                     YGYTSLISAGTHYNIDVELAQAIDAGEVVGPRLVPCSRNYMPAMSADGVMENEETCMA
                     RGPEDFRKGVLFDLERGVKIIKIFASGGHGAPEGLCMSAEEVKAVVEVAGDYGARVRA
                     HVAGKQQILDCLRAGVTIIDHGDEADEECIAAYLEHDAFVLPSGYVLLKAAELGGNTF
                     GFSEDLKDFESLCRLLPRLVDAGVKLVPGDDFGIWQIPHGDYAQELVCYAEHAGIPPL
                     ELIKWSTLHGGEMTGFEGIGTIAPGKVADLLILNDDPSRDIRVLTRPESVQAVLKGGQ
                     LVSGELPTPLAAVSAG"
     gene            complement(1298..2176)
                     /locus_tag="AC16VR_13300"
     CDS             complement(1298..2176)
                     /locus_tag="AC16VR_13300"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIR6012181.1"
                     /translation="MSDAQARGLPPDDPTTLITPELIEQYRRDGVICVRNALHPEWLM
                     LLEMGLERIMADPGQQKHLFYRGEPGEFIETIRNFDVAPEIRRLIYDSPIADMIGKLI
                     GSGNIWLYSDEFFVKEGGGCERTPWHQDLPYWPLEGEQIASMWISLDPLPREDCLEYV
                     AGSHQGPMFDGFDPQRVTEDPTLPHYGEGLPPLPDIEADRVSFNIVSWEIGPGDVILA
                     HPGVLHGGGATGIADRRRAITVRCYGDDVVYATRPPTRPTVPLTPGLGLKLKPGDPLR
                     HPYYPRLRPLPEHQRV"
     gene            2347..>3346
                     /locus_tag="AC16VR_13305"
     CDS             2347..>3346
                     /locus_tag="AC16VR_13305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HIR6012182.1"
                     /translation="MDLNTLTSFEQRYLWEPDYRQAPPWQRVLVGMARTLMLLFRDIF
                     HGDISLRAMSLVYTTLLSMVPLLALSFSILKGFGVQNQLDETLRAALAPLGEEKALEL
                     TENLITFVNNMNVGVLGAVGLLFLLYTVVSLMQKIEDSFNHTWRIRTPRTFGERFTTF
                     LSAITIGPLLIFTAIALTGSIMSTTVMTHVQEIAVVGPAIQIVVRLLPFVFVIGAFTF
                     LYVFIPNTRVRISAALTGGIVAGVLWELLGFGFASYASGASRYQLIYATFATAIFFLI
                     WLYLNWLILLIGASIAFYRQHPEVVASGLKEVHFSQQLTTRYVLSLLAKIGRRHYEK"
BASE COUNT          604 a         1083 c         1048 g          611 t
ORIGIN      
        1 gagggacaac atgccacgag ttctgtacag caatgcgaac ctgctcgacg gtgagcacgc
       61 cgccagaccc ggatcctcgg tcatcgtgga agacgggcac atccttgccg tcggcacccc
      121 ggagtccatg gcggccgccg cgtacgacga gctgatcgat ctcgagggcc gcacgctcat
      181 gcccgggctg gtcatgggcc actatcacgc cgcgtatcgc ggctacggtg aagagggcgg
      241 cagccagacg gccaccgccg tcgagcaagc gtttttcgcg ctcggcaacg tgcagattgc
      301 gctcaagtac ggctacacca gcctgatcag cgcgggcacc cactacaaca tcgacgtcga
      361 gctcgcccag gccatcgacg cgggcgaggt ggtcggcccg cggctggtgc cctgcagccg
      421 caactacatg cccgccatga gcgccgacgg cgtcatggag aacgaagaga catgcatggc
      481 gcgtggcccg gaggatttcc gaaagggcgt gctgttcgac ctcgaacgcg gtgtgaagat
      541 catcaagatc tttgccagcg gcggtcacgg cgcgcccgag ggcttgtgca tgagcgccga
      601 ggaggtcaaa gccgtggtgg aggtcgccgg cgactacggc gccagggtcc gagctcacgt
      661 cgccggcaag cagcagatac tcgattgcct gcgtgccggc gtcaccatca tcgatcacgg
      721 cgacgaggcg gacgaagaat gcatagcggc ctacctggag cacgacgctt tcgtgttgcc
      781 cagcggctac gtgctgctca aggcagccga gctggggggc aacacgttcg gtttcagcga
      841 ggacctcaag gactttgaga gcctgtgcag actgttgccc cgcctggtgg atgccggcgt
      901 gaagctcgtg cccggcgacg acttcggcat ctggcagata ccgcacggcg attacgccca
      961 ggagctcgtc tgctacgcgg agcacgccgg catcccgccg ctggagctga tcaaatggag
     1021 cacgttgcac ggtggcgaga tgacgggctt cgagggcatc gggaccatcg caccgggaaa
     1081 ggtggccgac cttctgatcc tgaacgacga tccgtcgcgg gacattcgcg tgctgacgcg
     1141 gcctgagagc gttcaggcag tgctgaaagg cgggcagctg gtgagcgggg agctgcctac
     1201 cccgctggct gcggtatccg caggataggc atccgccgca cacgggcaga gcgcaacgag
     1261 cggacccgca ggggtcgatg cgcccgctga tccctcctca gacccgctgg tgctcgggca
     1321 gcggccgcaa ccgcggatag tagggatggc gcagcgggtc tcccggtttc agctttagac
     1381 cgaggcccgg cgtgaggggc accgtgggcc gcgtgggcgg ccgggtggcg tagacgacgt
     1441 cgtccccgta acagcgaacg gtgatcgctc tgcgacgatc cgcgatcccg gtcgcaccgc
     1501 caccatgcag aacgcccgga tgcgcgagga tcacatcccc gggaccgatt tcccaggaga
     1561 cgatgttgaa gctgactcga tccgcctcga tgtccggcag cgggggcagc ccctcgccgt
     1621 aatgcggaag ggtcgggtcc tccgttaccc gctgggggtc gaaaccatcg aacatggggc
     1681 cctggtggga gcccgccaca tactccaggc aatcttcccg gggcagcgga tccagagaga
     1741 tccacatcga ggcgatctgc tctccttcca gcggccaata cggcagatcc tgatgccacg
     1801 gcgtccgctc acacccgccg ccttccttca cgaagaactc gtccgagtaa agccagatgt
     1861 ttccggaacc gatgagcttg ccgatcatgt cggcaatggg cgagtcgtat atgagccgcc
     1921 ggatctcggg cgccacgtcg aagttgcgta tggtttcgat gaattcccca ggctcgcccc
     1981 gatagaacag atgcttctgc tggccggggt cggccatgat gcgctcgaga cccatctcca
     2041 gcagcatcag ccactccgga tgaagggcgt ttcgaacgca gatgacgcca tcgcgccgat
     2101 actgctcgat cagctccggc gtgatcagcg tggtgggatc gtccggcggt agcccgcgag
     2161 cctgcgcgtc tgacattctc cctctccaga aaccctttgc cctaatctag cccagcccgg
     2221 caaacctcgc tacggctaag cagcgcgctg cgcggccgcg tcgtatgggc gcacccgcct
     2281 gggcgagggg ccttgacggc acccgggaaa tccctttccc ttcactccaa gggcaggggg
     2341 acgcacttgg atctcaacac tctcacatcg ttcgagcagc gctatctctg ggagccggac
     2401 tatcggcagg ctcccccatg gcagcgcgtg ctggtgggca tggcccgaac gctcatgctc
     2461 ctgttccggg acatctttca cggtgacatc agcctgagag ccatgagcct cgtgtacacg
     2521 acgctgctct ccatggtgcc gctgctcgcg ctgagcttct cgattctcaa gggattcggg
     2581 gtccagaatc agctcgacga gactttacgt gcagcgctgg cgcctctggg cgaggaaaag
     2641 gccctcgagc tgacggaaaa cctgatcacc ttcgtcaaca acatgaacgt gggcgtgctg
     2701 ggcgcggtcg gtctgctgtt tctgctctac accgtggttt cgctgatgca gaagatcgag
     2761 gattcattca atcacacctg gcgcattcgc acgcccagaa cgttcgggga acgtttcacc
     2821 accttcctct ccgccatcac catcgggccg ctgctcatct ttactgccat cgccctgacg
     2881 ggctcgatca tgagcaccac cgtgatgacc cacgtccagg agatcgccgt ggtcggcccg
     2941 gccatccaga ttgtcgtcag gcttctgccg ttcgtcttcg tcatcggcgc gttcaccttc
     3001 ctgtacgtct tcatccccaa tacgagagtg cgaatctcgg cggcattaac tggcggcatc
     3061 gtcgccggcg tcctctggga gctcctgggc ttcggatttg cctcctacgc cagcggcgcc
     3121 tcccgctacc agctgatcta cgccacgttt gctacggcca tattctttct catctggctg
     3181 tatctgaact ggctgattct tctcatagga gcaagcatcg ccttctatcg gcagcatccg
     3241 gaggtggtgg cctcgggctt gaaggaggtg cacttcagtc agcagctgac tacccgctac
     3301 gtgctctccc tgcttgcaaa gatcggtcgc cggcattacg agaaga
//
LOCUS       DBNHQR010000585         5017 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_944080, whole genome shotgun sequence.
ACCESSION   DBNHQR010000585 DBNHQR010000000
VERSION     DBNHQR010000585.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5017)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_944080"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(268..1413)
                     /locus_tag="AC16VR_13310"
     CDS             complement(268..1413)
                     /locus_tag="AC16VR_13310"
                     /inference="COORDINATES: protein motif:HMM:NF019640.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6012183.1"
                     /translation="MNPVETAAGFAAEIRRRAPEIEQHRRMPGDLANRMAGAGLFRLL
                     VPELYGGLQVEPLAFFETLKETARADGAVGWCQMIGVTTGMMAASLPDEWSRTIYGCN
                     PDTITSGVTAPLGIAVAEGDGLRVSGRWPFGSGCEVSDWICGGCQLMEDGEPRKNRFG
                     SPEIALVFFPADDVTIHDTWHTSGLCGTGSHDIEVKDAEVPAGRWVTMGKRARVDAPL
                     YRFPTLGLLALGVSAVALGIAEHAIEAFTTLARDKVPTGSARPLSARSGAQRDLARAM
                     AAVQSAAALTREAIEDAWGEAHREGSLGLETKARLRLAATNNAWSAAEAVDLLYHAAG
                     GSSIYAHSALQRCFRDVHVATQHLMVAQPIYEVVGKVALGIDPKTLL"
     gene            complement(1410..1889)
                     /locus_tag="AC16VR_13315"
     CDS             complement(1410..1889)
                     /locus_tag="AC16VR_13315"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6012184.1"
                     /translation="MAELKVCDVKLAAIRDAGLIARMSRDYIEHGLRWRWRAPQIRAK
                     IRHPETAVIVAEVELGGVRFIGGFASMDFGEDKAALNLLAVHPNLRRRGIGGNLVEWL
                     HKSAGVAGCQHIELQVRAGNVSARRFYGRMGYTEEALINGYYDGKEAAYQMKFRLAQ"
     gene            complement(1967..2416)
                     /locus_tag="AC16VR_13320"
     CDS             complement(1967..2416)
                     /locus_tag="AC16VR_13320"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6012185.1"
                     /translation="MDNQLIAEIQRDCEALSLAYARAIDFRDYDHFVSLFTDDAVLDL
                     GRPLRGREEIHAAISARPDELRSRHVITNVFVDVVDENTARGIAYLTLYRHIADKPSI
                     PGPGSEPAPTTLPAAVGHYQDRFARGPDGWRFEARSLHLAFRDPAAF"
     gene            2521..3303
                     /locus_tag="AC16VR_13325"
     CDS             2521..3303
                     /locus_tag="AC16VR_13325"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6012186.1"
                     /translation="MERMSLEFVGKVAVLKFNHPEVMNAVGSQMLLDFADALKDIAHP
                     GNGARCLLLTGEGRGFCAGANLQDGERSERRGGAGDGLRASYHPILLTLRDLNMPMVT
                     AVNGAAAGVGMSFAMMGDIVCASSNAFFLQAFARIGLVPDGGSTFLLPRLVGWGRAME
                     LSMLAERLPAEKAHEWGLVNRLFDDNEALLEGAMAIADRLASGPRSLALIRQAYWNTW
                     HNAFEQQLDLEARLQNQAGASRDFAEGVAAFLEKRDAEFTGE"
     gene            complement(3307..4224)
                     /locus_tag="AC16VR_13330"
     CDS             complement(3307..4224)
                     /locus_tag="AC16VR_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007349674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HIR6012187.1"
                     /translation="MLLITHRDCLLHEMQHRHPERPDRLRAVLQLLESSGLSRDLKHA
                     EALPASQEALRAVHDPDYLRTLSESVPESGLLPVDPDTAMGPSSLRAAALAAGAVADG
                     VRAVLEGDQSTVFCAVRPPGHHAEHDAAMGFCFYNSIALGAVTALEDPRVERVAVLDF
                     DVHHGNGTVDIFKDRPEVLVCSSFQHPFYPHRYADLARPNIVNTPLPAGTAGADFRRA
                     VEAQWLPAIDNHQPQLIMVSAGFDAHRLDPLAQLELCEDDFRWITRLIVASAKTHANG
                     RIVSALEGGYDLAALAASAHAHIEELLAA"
     gene            complement(4265..>5017)
                     /locus_tag="AC16VR_13335"
     CDS             complement(4265..>5017)
                     /locus_tag="AC16VR_13335"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR6012188.1"
                     /translation="PSALERDEFSLLLQPQWSLQDNRLTAVEALLRWQGRDIGGLAPS
                     HFIPLVEQQGQMARVGDWVLERACRETAAWLPRMVQPILLTVNVSPQQFHGRSIVTQI
                     ARNSAEHRLNPASLELELSQRNMLRLVDDYREQMYTLRDWGVRFAIDNLGGGLIEPEK
                     LLRCPVDTLKIDRAVVSALSEDTQAADLAAQICELGARFNLRVVGVGVETEEQLKLLA
                     EWGCTDAQGLLVSRPVPVTGIQHLMNRLADLN"
BASE COUNT          836 a         1587 c         1739 g          855 t
ORIGIN      
        1 gaacatgtgc cccgcatcgg cgagcagggt cagggagttg gccagcagcc caccgatgac
       61 ctcgacgacc atgaagccgg cgatgagcac gaaagcgcgg ccaacgatga cctcgttgtc
      121 cctggtatga atgtggttgt ggtcgtggcc cacgtttacg cgttgtcctg cagccgactc
      181 acgaacgccc gtgattatac cgactcaccg cgctcggtgg tccgctccgt cccgggcgtc
      241 gtgggtcacg tcgccacggg actcggctca gagcagcgtc ttcggatcga tgcccagggc
      301 gaccttgccg accacctcgt agataggctg cgctaccatc agatgctgcg tggcgacgtg
      361 cacgtcgcga aaacaccgct gcagcgcgct gtgcgcgtaa atcgacgatc ctcccgcggc
      421 atggtagagg aggtccaccg cctcggccgc cgaccaggcg ttgttggtgg cggccagcct
      481 gaggcgggcc ttggtttcca ggccgaggga tccttcgcgg tgggcttccc cccaggcgtc
      541 ttcgatggcc tcgcgggtga gcgccgccgc tgactggacg gcggccatcg ctctggccag
      601 gtcgcgctgg gccccagacc gggcggaaag aggccgggcg ctgccggtgg ggaccttgtc
      661 ccgggcgagc gtggtgaaag cctcgatggc gtgctctgcg atgcccagtg caaccgcgga
      721 cacgccaagg gcgagcaagc ccagcgtagg aaatcggtag aggggcgcgt ccacccgcgc
      781 gcgcttgccc atggtgaccc atcgccccgc gggaacctcg gcgtccttta cctcgatgtc
      841 gtggctgccg gtcccgcata agcccgacgt gtgccaggta tcgtgaatcg tcacgtcgtc
      901 tgcgggaaag aaaaccaggg cgatctcggg cgagccgaac cggttcttgc gcggctcgcc
      961 atcttccatg agctggcagc cgccgcagat ccaatccgag acctcgcacc cgctgccgaa
     1021 gggccaacgg ccgctgaccc tgagaccgtc gccttcggca acggcgatgc ccagcggggc
     1081 ggtgacgccg gaagtgatgg tatcggggtt gcaaccgtag atcgtgcgag accattcgtc
     1141 gggcagcgag gcggccatca tcccggtggt cacgccgatc atctggcacc agccgaccgc
     1201 cccgtcggcg cgggccgtct ccttgagggt ttcgaagaaa gctagaggct cgacctgcag
     1261 gccgccgtag agctccggga cgagcaggcg gaacagtccc gcaccagcca tgcgatttgc
     1321 gaggtcgccg ggcatccggc gatgctgctc gatctccgga gcacggcggc ggatttccgc
     1381 agcgaagcct gcggcggtct cgaccgggtt cattgggcca ggcggaactt catctggtaa
     1441 gcggcctcct tgccgtcgta gtagccgttg atgagggctt cctcggtgta gcccattcga
     1501 ccatagaaac gacgcgcaga cacgttgccc gctctgacct gcagctcgat gtgctggcag
     1561 ccggccacgc ccgcgctctt gtgcagccac tcgacgaggt ttccaccgat gccgcgccgt
     1621 ctcaaattcg ggtgcacggc aagcaggttc aatgcggcct tgtcctcgcc gaaatccatc
     1681 gaggcaaaac cgccgatgaa gcgaacgccg ccgagctcca cctccgcgac gatgacggcc
     1741 gtttcgggat gccggatctt ggcgcggatc tgcggcgcgc gccagcgcca gcggagcccg
     1801 tgctcgatgt agtcccggga catgcgcgcg atcagccccg cgtctctgat tgcggccagc
     1861 ttgacatcgc agactttcaa ttcggccatt gaccgattat tgccaatccc ccatcccgag
     1921 gggaacgggt ccgggcgccg aggttgcaaa cgacttgtag aaccggtcag aaggcagccg
     1981 ggtcgcggaa cgccagatgc aggctgcgcg cctcgaaccg ccatccgtcc ggcccgcggg
     2041 cgaaccggtc ctggtagtgg ccgacggcgg cgggaagcgt ggtcggcgcg ggttcgcttc
     2101 cgggacccgg tatcgacggt ttgtcggcaa tgtggcgata gagggtcaga taagcgatgc
     2161 cgcgggcagt gttttcatcg acgacgtcta cgaacacgtt ggttatgacg tgacgcgagc
     2221 gcagctcgtc tggccgcgcc gagatggcgg cgtgaatctc ctcgcgcccg cgcagcgggc
     2281 ggccgaggtc gagcacggcg tcgtcggtga aaagggagac gaaatggtcg tagtcccgga
     2341 agtcgatggc gcgggcgtat gccagggaga gcgcttcgca gtcgcgctgg atttccgcga
     2401 tcagctgatt gtccacgtcg ggtttctccc tcctggtttg agcctggcca tcgggttggt
     2461 tatcgttccc gatccgtcgc agatcgacaa gtcggtcgcg ctttcagatt gggggaacca
     2521 atggaacgaa tgtcgctgga gttcgtgggc aaggtggccg tgctcaaatt caatcatccc
     2581 gaggtaatga atgcggtcgg cagccagatg ctgctggatt tcgccgacgc gttgaaggac
     2641 atcgctcatc cgggcaatgg cgcccgctgc ctgctgctca ccggcgaggg gcgcggcttc
     2701 tgcgccggcg ccaaccttca ggatggcgag cgctccgagc gccgcggcgg cgccggtgac
     2761 ggcctgcgtg ccagctatca tccgatactt ctaaccttgc gggatctcaa catgccgatg
     2821 gtcaccgccg tcaacggggc cgctgccggc gtcggcatga gctttgccat gatgggcgat
     2881 atcgtctgcg catcttcgaa cgccttcttt ctgcaggcgt tcgcgcgaat cggtctggtg
     2941 cccgacggtg gctcgacctt cctgctgccc cgcctggtgg gatggggccg cgccatggag
     3001 ctctccatgc tggccgagcg gctgccggcg gagaaagccc acgagtgggg gctggtgaac
     3061 cgtttgttcg acgacaacga ggccctgctc gaaggtgcca tggccatcgc cgaccggttg
     3121 gccagcgggc cgagatcgct tgccctgatt cgccaggctt actggaacac gtggcacaac
     3181 gcgtttgagc agcagctcga tctcgaagcc agactgcaga atcaggccgg cgcgtcgaga
     3241 gacttcgccg agggcgtggc cgcgttctta gagaagcgcg acgcggagtt caccggcgag
     3301 taacgactac gcggcgagca gctcctcgat gtgcgcatga gccgatgcgg ccagcgcggc
     3361 gaggtcgtaa ccaccttcta aagcggagac gatgcgtccg ttggcatggg tcttcgcgct
     3421 ggcgacgatg agccgggtga tccagcggaa atcgtcctcg cacagctcga gctgagccag
     3481 ggggtcgagg cggtgggcgt cgaaccccgc tgagaccatt atcagctggg gctggtggtt
     3541 gtcgatggcg ggaagccact gcgcctccac ggcccggcga aagtcggcgc ctgcggtgcc
     3601 tgcgggcagg ggcgtgttga cgatgttggg gcgcgccagg tctgcgtagc ggtgggggta
     3661 gaacgggtgc tggaagctcg agcagaccag cacctcgggc cggtccttga atatgtccac
     3721 cgtgccgtta ccgtggtgga cgtcgaagtc caggacggcg acgcgctcta cgcgggggtc
     3781 ttcgagggcg gtgacggccc ccagggcgat gctgttgtag aagcagaaac ccatggccgc
     3841 gtcgtgctcg gcgtgatgac ctggagggcg aacggcacag aacactgtgg attggtctcc
     3901 ctcgaggacg gcgcgaacgc cgtcggcaac cgcgccggcg gccagggcgg ccgcccgcag
     3961 gctggatggc cccatggccg tgtccggatc aacgggcagc agtccggact cgggcaccga
     4021 ttcggacagc gtgcgcaggt aatccggatc gtgcacggct cgcagcgctt cctggctcgc
     4081 cggcaaggcc tctgcatgct tcaggtcccg ggacagaccg ctggactcga gcagttgcag
     4141 caccgcgcga agacgatcgg gtcgctcggg atgccggtgc tgcatctcgt gcagcaggca
     4201 gtcccggtga gtgatcagca gcatcggccg atggtagcag ggtccccgga aggaagcagc
     4261 ggggtcagtt taagtcggcg aggcgattca tcaggtgctg gattccggtt accggtaccg
     4321 gacgagagac gagcagcccc tgggcgtccg tgcagcccca ctcggcgagc aatttgagct
     4381 gctcctcggt ctcgacgcca accccgacga cgcgcaggtt gaatcgcgcc cccaactcgc
     4441 agatctgcgc ggcgagatcg gccgcctggg tatcctcgga caacgcgctc accacggctc
     4501 ggtcgatctt gagcgtgtcc accgggcagc gcagaagttt ctcgggctcg atgagcccgc
     4561 cgccgagatt gtcgatggca aagcggacgc cccagtcgcg cagggtatac atctgctcgc
     4621 ggtagtcgtc caccagtcgc agcatgttgc gttgcgacag ctccagctcc agggacgcgg
     4681 ggttgagccg gtgctcggcg ctgttgcggg cgatctgggt gacgatgctg cggccgtgaa
     4741 actgctgggg cgatacgttt accgtcagca gaatgggctg caccatgcgg ggaagccacg
     4801 cggcggtctc gcggcaggcg cgctctaaaa cccagtcacc cacccgggcc atctgtccct
     4861 gctgctcgac caggggaatg aagtggctgg gggcaagccc gccgatgtcg cggccctgcc
     4921 agcgcagcag cgcctcaacg gcggtcagcc ggttgtcctg cagactccac tggggctgca
     4981 gcagcagcga gaactcgtcc cgctccaggg cggaagg
//
LOCUS       DBNHQR010000586         5779 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1577692, whole genome shotgun sequence.
ACCESSION   DBNHQR010000586 DBNHQR010000000
VERSION     DBNHQR010000586.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5779)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1577692"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="AC16VR_13340"
     CDS             <1..308
                     /locus_tag="AC16VR_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017700834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HIR6012189.1"
                     /translation="LSGQIPLVPATMDMVAGDVIEQAHQVFRNLRAVASAAGGSLDDL
                     VKVTVYLTDLNDFQSVNEVMTAYFSEPYPARAAIGVAALPRGAAVEVEGVMAHPGDG"
     gene            338..2350
                     /gene="recG"
                     /locus_tag="AC16VR_13345"
     CDS             338..2350
                     /gene="recG"
                     /locus_tag="AC16VR_13345"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019694287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIR6012190.1"
                     /translation="MIAEQRVESLKGVGPSLKAKLERLGIFRVGDLLLHLPIRYQDRS
                     RVVPLRDVHPQQECLVQGQVVDSSLGYGRRRSWLVTIEDGTGYLGLRFFHFSKRQQSG
                     LQPGTYVRCFGEIRFGPRGGGPGLEMAHPEYRAFTSEPPDPDPELTPVYPTTKGLGQN
                     RLRGLIDQALSLEWPSDSGAPYQTLAYLHRPPADAPAADIERAQERVALDELTAYYLV
                     MKQRQRDRAKSRALPLPQSQQLGRELLRRLGFRLTHAQRRVITEVLEDLSSETPMLRL
                     LQGDVGSGKTVVAAFAAIRAAEHGRQTALMAPTEILAEQHYVNFSAWLEPLGIPVVLL
                     TGSQSAKERAARQQALEEGEALVAVGTHALFQDKVNFNALALTIVDEQHRFGVHQRMA
                     LRSKGAAPHQLVMTATPIPRTLTMALYADMDVSVLDELPPGRTPIDTRVISDARKEEV
                     IRSAADAMARGEQVYWVCPLIEDSEQLDAAAAEPTAELLAERLEPPIGLLHGRMTGEQ
                     KAKAMMDFKEGRTRLLVATTVIEVGVDVPNATLMVIENPERMGLAQLHQLRGRVGRGE
                     KASRCLLVYKTPLSAAARQRLSVMRESQDGFHIAEKDLELRGPGELLGARQTGEQQFR
                     VADLARHSHLIAEVLARGERLLQTDPTTARELLNAWAPEDNGHIAV"
     gene            complement(2372..3640)
                     /locus_tag="AC16VR_13350"
     CDS             complement(2372..3640)
                     /locus_tag="AC16VR_13350"
                     /inference="COORDINATES: protein motif:HMM:NF012593.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HIR6012191.1"
                     /translation="MNLTIRILLGMGLGLVLGSSIQAAEFDPSHWVHVYLVGGLIDAG
                     GQIFVRSLQLMVVPLVFVSLVCGAASLSATDSIGRVAGKTVALYLFTTAVAISIALLL
                     ATIVQPGAGVAPASLGTDFQPAEPTSIKDTLVSIIPTNPVSAMAEGRMLQIILFALLL
                     GLALSRSGQAGRRLAGVFDDLNEALMKLITLLIKLAPIGVFCLMAKLFATLGWSEITR
                     LLQYFLTVVGALLIHVTLVYPTLLMVLGRVSPIRFFGKMREPLLVAFSTASSGATLPV
                     TLRTVEERVGVKNEVASFVVPLGATINMDGTAIMQGVATVFIAQLYGIELGMSQFLII
                     ILTATMASVGTAGVPGVGMIMLVMVLEQVGLPLDGIVFILAVDRLLDMLRTAVNVAGD
                     GMVATVVARSENELDLDTFEDPDAGRVIGS"
     gene            complement(3657..3824)
                     /locus_tag="AC16VR_13355"
     CDS             complement(3657..3824)
                     /locus_tag="AC16VR_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0043448 - alkane catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HIR6012192.1"
                     /translation="MRKWQCIVCGWIYDEAEGYEEEGIAPGTAWEDVPEDFVCPECGV
                     SKEDFEMIEVG"
     gene            3984..4892
                     /gene="ubiA"
                     /locus_tag="AC16VR_13360"
     CDS             3984..4892
                     /gene="ubiA"
                     /locus_tag="AC16VR_13360"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007160213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     polyprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HIR6012193.1"
                     /translation="MEKTRQPPLSAVIAYARLMRLDRPVGTLLLLWPTLAALWVAAEG
                     LPPVDLIVVFAVGTFLMRAAGCVVNDYADRNLDPGVARTANRPLATGAVSTNAALGAF
                     FVLAFLALTLLLFLNTLTRWFAVAGLAIAVAYPFMKRWTYLPQVVLGAAFSWGIVMAF
                     TAVRHVVPEPGWLLFLASVLWIVAYDTMYAMVDREDDLRVGIKSTAILFGEADRLMIG
                     LLQTGTILTLVLVGQRLGYHSAYFWGIAVAAGLFCYQQYLIRDRSREGCFRAFANNVW
                     VGFSLFVGVAWETMTRGAFQPGGGLS"
     gene            4946..5407
                     /locus_tag="AC16VR_13365"
     CDS             4946..5407
                     /locus_tag="AC16VR_13365"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HIR6012194.1"
                     /translation="MAWLTALMVVLMVTVVVLRYAFDQGAIPIQEAIVYLHATVIMLG
                     IPCALRDGVHVRVDILHSRLSERARGIIDVAGHLLFLVPTCLLILYFSIPYARNAWLV
                     LEGSPEVGGIPAVFLLKTLIPVMAVALLLQGLAETVRRLPIILPSHFGSER"
     gene            5412..>5779
                     /locus_tag="AC16VR_13370"
     CDS             5412..>5779
                     /locus_tag="AC16VR_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HIR6012195.1"
                     /translation="MVELIPIFMFLAVFAALLAGYPVALTLAGVALLFALGGMAGGTF
                     DGRDLGFLPGRLFGIITNPTLIAVPLFVFMGVMMEKTRIAENLLSSLSRLMAGTPGGL
                     AFAVIVVGMLMAASTGIVGAS"
BASE COUNT          989 a         1949 c         1829 g         1012 t
ORIGIN      
        1 ttctctccgg tcagattccg ctggttccag cgaccatgga catggttgcg ggcgacgtga
       61 tcgagcaggc gcatcaggta ttcaggaatc tgcgcgctgt tgccagcgct gcaggcgggt
      121 ccctggacga tctggtgaag gtcaccgtct acctcacgga tctcaacgac tttcagtcgg
      181 tcaatgaggt aatgacagcc tatttcagcg agccgtatcc cgcgcgcgcc gcgatcggcg
      241 tggccgcgct gccacgtggc gcggccgtgg aggtggaagg cgtcatggct caccccggtg
      301 acggctgatt cgtcaaagcc ctgagccggc aggcgccgtg atcgctgaac agcgcgtgga
      361 atccctgaaa ggtgtcggac cgagcttgaa ggcgaagctc gagcgcctgg gcattttccg
      421 ggtcggcgac ctgctgctgc acctgcccat tcgctaccag gatcgcagcc gcgtggtccc
      481 ccttcgcgac gtccacccac aacaggagtg cctggtgcag gggcaggtgg tggattcatc
      541 cctcggctac ggtcgccgtc gcagctggct ggtgaccatc gaggacggca ccggctacct
      601 gggcctgaga ttctttcact tctcaaagcg tcaacagtcc gggctgcagc cggggacgta
      661 cgtgcgttgc ttcggcgaga tccgtttcgg cccccggggc ggcggtcccg ggctggagat
      721 ggcgcacccg gagtaccgcg cctttacctc cgagcccccg gatccagatc cggagctaac
      781 gcccgtctac cccacgacca agggtctagg gcagaaccgc ctgcgcgggc tgatcgatca
      841 ggccctttcc ttggaatggc ccagcgactc cggcgcgcca taccagaccc tcgcctatct
      901 gcatcggccg ccggcggacg cgcccgccgc cgacatcgaa cgagcccagg agagggtcgc
      961 cctggacgag ctcaccgcct actacctggt catgaagcag cgccagcgcg atcgcgccaa
     1021 atcccgcgcg ctgccgctgc cccaatccca gcagctcggc cgggagctct tgcgcaggct
     1081 cggattcagg ctcacccatg cccaacgaag ggtgattacg gaggtgctgg aggacctgtc
     1141 cagcgaaacc cccatgctgc ggcttctgca aggcgacgtc ggctccggca agaccgtggt
     1201 cgccgctttc gcagccatta gggcagccga gcacggccgc cagacggcat tgatggcgcc
     1261 gaccgaaatc cttgcggagc agcactacgt gaacttctcc gcctggctcg aaccgctcgg
     1321 gatccccgtc gtgttgctca cgggttccca gtcggcgaaa gaacgtgccg caaggcagca
     1381 ggcgctagag gagggcgaag cgctggtggc ggtgggcacc catgccctgt tccaggacaa
     1441 ggtgaacttc aacgccctgg cgctgaccat cgtcgacgag cagcatcggt tcggcgtgca
     1501 ccagcgcatg gcgctgcgca gcaagggggc cgcgccccat cagctggtca tgaccgccac
     1561 gccgattccg cgcacgttga ccatggccct gtacgcggac atggacgtct ccgttctcga
     1621 cgagcttccg ccggggcgga cgcccatcga cacccgtgtg atcagcgacg cccgcaagga
     1681 ggaagtgatt cgaagcgcag ccgatgcgat ggctcggggt gagcaggtct actgggtatg
     1741 ccccctcatc gaggattccg agcagctcga cgctgccgct gcggaaccca ctgccgagct
     1801 gctcgccgag cgcctcgagc ccccgatagg cctgcttcat ggtcggatga ccggcgagca
     1861 gaaagccaag gccatgatgg acttcaagga gggccgcacg cggctgctgg tcgcaaccac
     1921 ggtgatcgag gtcggcgtag acgtgccgaa cgccacgctg atggtcatcg aaaacccgga
     1981 gcgcatgggc ttggcacagc tgcatcagct gcgcggcagg gtcgggcggg gagagaaagc
     2041 atcgcgatgc ctgctggtct acaagacacc gctgagcgcg gcggcacgcc agcgtctttc
     2101 ggtcatgcgg gagagccagg acggctttca catcgccgag aaggacctgg agctgcgcgg
     2161 accgggcgag ctgctgggcg cgcgccagac gggcgagcag cagttccgag ttgccgatct
     2221 cgcccgccac agccatctga ttgccgaggt gctggcacgc ggcgaacgcc tcctgcagac
     2281 ggacccgaca acggcccggg agcttctcaa tgcctgggca cccgaggaca acgggcacat
     2341 cgccgtgtag gagggccctt ccgggcagct cttatgagcc gatcacacgg ccggcatcgg
     2401 ggtcctcgaa cgtgtcgaga tccagctcgt tttcggaccg ggccaccacg gtcgccacca
     2461 tgccgtcacc ggccacattg accgccgtcc tcaacatgtc cagcagccgg tccacggcca
     2521 agatgaagac gatgccgtcg agaggcagcc cgacctgctc cagcaccatg accagcatga
     2581 tcatgccgac gccggggacc cccgccgtgc cgacggaggc catggtcgcc gtgaggatga
     2641 tgatgaggaa ctgcgacatt cccagctcga tgccgtacag ctgcgcgatg aacaccgtcg
     2701 ccaccccctg catgatggcg gttccatcca tgttgatggt tgcgcccagg ggcacgacga
     2761 agcttgccac ttcgtttttc acgccaaccc gctcctccac ggtgcgcagg gtgaccggca
     2821 gggtcgcccc gctcgaggcg gtgctgaagg ccaccagcag cggctcgcgc atcttgccga
     2881 agaaacggat cgggctgacc ctgcccagca ccattagcag cgtcgggtag accagggtga
     2941 cgtgaatgag caaagcgccc accaccgtca ggaaatactg caggagccgc gtgatctccg
     3001 accagcccag ggtggcgaac agcttcgcca tcaggcagaa gaccccgatg ggcgcgagct
     3061 tgatcagcag cgtgatcagc ttcatcaacg cttcgttcag atcgtcgaaa acgccggcca
     3121 gccgccggcc ggcctgcccc gaccggctca gggccaggcc gagcagaaga gcgaacagga
     3181 tgatctgcag catcctgcct tccgccatgg cgctaaccgg gttcgtcggg atgatggaga
     3241 ccagcgtgtc cttgatgctc gtcggctccg ccggctggaa gtcggttccc agggaggccg
     3301 gcgcgacgcc ggcaccaggc tgaacgatcg ttgcaagcag cagggcgatg gaaatggcca
     3361 cggccgtggt gaacagataa agggcaacgg tctttccggc cacccgaccg atgctgtccg
     3421 tcgccgacag gctggcagca ccgcacacca acgacacgaa taccagggga accaccatga
     3481 gctggaggct gcgaacgaat atctggccgc cagcgtctat caggccgccg acgaggtaga
     3541 cgtggaccca atgggaagga tcgaattccg ccgcctggat cgaagagccg aggacgagcc
     3601 ccaagcccat gccgagtaga atgcggatcg taaggttcac gacgttctaa tcggcctcag
     3661 ccgacctcga tcatctcgaa gtcttctttg ctgacgccgc actccgggca aacgaaatcc
     3721 tcgggcacat cctcccaagc cgtacccggc gcaatgccct cttcctcata accctcggct
     3781 tcgtcgtaga tccagccgca gacgatgcat tgccattttc gcattcttgg tcgcttccct
     3841 ttgttcaagc cggcgccggc gcaaaggcgc gataaactac tcatcccccg cggaaaaatc
     3901 aatcgtgcgg ccggcggccg cctggcctgc ctggtggcga aacgctacac tccgccgcca
     3961 agctcaagcc cgcccgcgcg aacatggaga aaacccgtca gccgccgctc tccgccgtga
     4021 tcgcgtacgc gcgcctgatg cgcctcgacc ggcccgtggg caccctgctg ctcctgtggc
     4081 cgaccctggc cgctctctgg gtagccgccg aggggctgcc gcccgtggat ctcatcgtgg
     4141 tctttgccgt cggcacgttt ctgatgcggg ccgccggctg cgtggtgaat gactacgccg
     4201 atcgtaacct ggatcccggc gtcgccagaa ccgctaatcg accccttgcg accggagccg
     4261 tcagcaccaa cgccgcgctg ggcgccttct tcgtgctggc cttcctcgcc ctgaccctgc
     4321 tgctcttcct caacacgctg acccgatggt tcgccgtcgc aggcctggcc atcgcggtgg
     4381 cctatccctt catgaagcga tggacctacc ttccgcaggt ggtgctgggc gccgcgttca
     4441 gctggggcat cgtcatggcg tttaccgcgg tacggcacgt ggtgccggag cccggctggc
     4501 tgcttttcct ggcaagcgtt ctctggatcg tcgcctacga caccatgtac gccatggtgg
     4561 accgggagga cgatctgcga gtggggatca agtccacggc catactgttt ggcgaggccg
     4621 accgtctgat gatcgggctt ctgcagaccg gcacgatcct caccctggtc ctggtcggcc
     4681 agcggctcgg ctaccacagc gcctacttct ggggcatcgc cgtcgcggcc ggcttgttct
     4741 gctatcagca atacctgatc cgggaccgaa gccgggaggg ttgcttccgc gccttcgcca
     4801 acaacgtgtg ggtcggtttt tcgctgttcg tcggcgtcgc atgggaaacc atgacccgcg
     4861 gcgccttcca gccaggtggg gggctgagct gaacacacgg ctgctcgagc acgccgatcg
     4921 cgtcaccgag gcgacggccc gcagcgtggc ctggctaacc gccctcatgg tcgtgctgat
     4981 ggttaccgtc gtcgttctgc gctacgcgtt cgaccagggc gcgataccga ttcaggaagc
     5041 catcgtctat ctgcacgcca cggtgatcat gctcggcatt ccctgcgcct tgagagacgg
     5101 cgtccacgtc agggtggaca tccttcactc gcgcttgagc gaacgcgccc gggggatcat
     5161 cgacgttgcc gggcacctgc tgttcctcgt gcctacctgc ctgctaatcc tctatttcag
     5221 cattccctat gcccgcaacg cgtggctcgt gctcgaggga tcacccgagg tcggcggcat
     5281 tccggccgtc tttctcctga aaacgctcat tccggtcatg gcggttgccc tgctgctgca
     5341 gggcctcgcg gagaccgtgc gccggctgcc catcattctg ccctcccatt tcggtagcga
     5401 gcgctgacaa cgtggtcgag ctcataccga tcttcatgtt cctggccgtc ttcgccgccc
     5461 tgctggccgg ataccccgta gcgctgaccc tggccggggt tgccctgctg ttcgccctgg
     5521 gcgggatggc tggcggaacc ttcgacggcc gtgatctggg tttcctgccc ggccggctgt
     5581 tcggcatcat cacgaacccc acgctcatcg cggtgccgct gttcgtcttc atgggcgtga
     5641 tgatggagaa gacgcggatc gccgagaacc tgctcagcag cctttcgcgg ctgatggcgg
     5701 gaacgcccgg ggggctggct tttgccgtca tcgtggtcgg catgctcatg gctgcgagca
     5761 ccggtatcgt cggcgcctc
//
LOCUS       DBNHQR010000587         3909 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1385811, whole genome shotgun sequence.
ACCESSION   DBNHQR010000587 DBNHQR010000000
VERSION     DBNHQR010000587.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1385811"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1268)
                     /locus_tag="AC16VR_13375"
     CDS             complement(<1..1268)
                     /locus_tag="AC16VR_13375"
                     /inference="COORDINATES: protein motif:HMM:NF015573.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HIR6012196.1"
                     /translation="MTSTFPGVIAFAFMVCMLLAGTVLRAKVPLLRRALVPASLVGGI
                     LGFALVSAGLSFGYESSDFTVFTFHFFTLSFMSLCLTGGSRERGKNTSIIPGGMWMSV
                     VWVVSLVTQGLVGLGALVGYNVLTGGELSSFLGLLATHGFTQGPGQALAYASIWESEL
                     GVVHAINFGIIYASAGFIVAFAVGVPVARHAIKAGRNVNRVARIDDEFQAGILNADSD
                     VSAGRQITHSANIDTLAFHIAVLGVAYLVTDQYLSFMRPIADGWAIGDVNFGVLFSHN
                     LFFVHGLMVCVIMRSLLDRFGLGHYIDDETQRRITGTSVDFMVVATIMSIHFGLLSEF
                     IVPILVVCLAVSAVTVVLCFGLGRHLPNLSAERSLTSFGCCSGSTGSGLLLLRIVDPD
                     LSTGIAKELAFFNIAILVLSFHVLGFMAPIL"
     gene            complement(1311..3098)
                     /locus_tag="AC16VR_13380"
     CDS             complement(1311..3098)
                     /locus_tag="AC16VR_13380"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR6012197.1"
                     /translation="MYDRATTYGSDRVHEGPCHEHGPHGCPCCAATLGELLRRVGLDV
                     EAQAGRSVPAAPRAPVPTLFCNARVLTMDDAGSEFDTLLISDGRIQWVGNADADAVPE
                     VGDDTVRVDCNGRTILPGFVEPHMHLSAIAMLRRFENVGPFRYPTVSGALARLKEVAA
                     DTDPGAWIIGRQFDPSLQEGPGTLTASMLDEVSTTHPIFVYNASLHFGYCNSLALEIA
                     GITRDTPDPEGSPFGRLPDGTPDGVLKGGVSQAMVARHNPMLREHDLVEGCLDVFRHA
                     NSVGFTTICDQGTGAMQGAKELALYEALRESNRMTARFRYSLFNTLAADWEASDVSFG
                     TGDSWVRASAWKIVSDGSNQGLSGLQREPFMNADSHGIAYIEPEALNEAVKRRLGQGW
                     PVAVHANGDLAIDRVLDAFQAARAAGLDPARARCRIEHCSILHDEQIERIADLGLSPS
                     FLIGHVYWWGKAFRDEIFGLEKASLLDRTAACEAKGVRWTLHSDEPVTEMGPLRCIEN
                     AVTRRLWRTDDEVLAPHECVGVEQALRAMTRDAAWQCHSDHEIGTLEPGKLADLVVLE
                     EDPRSVPPSRLGAVRVLETWVGGERVYQA"
     gene            3224..3358
                     /locus_tag="AC16VR_13385"
     CDS             3224..3358
                     /locus_tag="AC16VR_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012198.1"
                     /translation="MTTGMTVMIVMTTLAVTVGVVALRVSWRIVNRLSEERPQNEEER
                     "
     gene            3496..3786
                     /locus_tag="AC16VR_13390"
                     /pseudo
     CDS             3496..3786
                     /locus_tag="AC16VR_13390"
                     /inference="COORDINATES: protein motif:HMM:NF041106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          776 a         1237 c         1293 g          603 t
ORIGIN      
        1 agaatcggcg ccatgaagcc gagcacatga aagctcagca ccaagatggc gatgttgaag
       61 aacgcgagct ccttggcgat gcccgtgctc aggtccggat cgacgattct gagcagcagc
      121 agcccacttc ccgtggatcc ggagcaacag ccgaacgagg tcagcgagcg ttcagccgaa
      181 aggttgggca ggtgcctgcc aagaccaaag cacaacacca cggttacggc agacacggca
      241 aggcagacca cgaggatcgg cacgatgaac tcggacagca gcccaaaatg aatgctcatg
      301 atggtggcga cgaccatgaa gtccaccgac gtgccggtta tccgccgctg ggtctcgtca
      361 tcgatgtagt ggcccaggcc gaaacggtcc agcagcgatc gcatgatcac gcagaccatc
      421 aggccgtgga cgaagaaaag gttgtggctg aagagaacac cgaagttcac atccccgatg
      481 gcccacccgt cggcaatcgg ccgcatgaag ctcaagtact ggtcggtcac gagataggcc
      541 acgccgagaa cggcgatgtg gaacgccagg gtatcgatgt tggcggagtg ggtgatctgt
      601 cggcctgcgg agacatcgga gtcggcgttc agaatgcctg cctgaaactc gtcgtctatc
      661 cgagcgaccc gattcacgtt gcgtcctgcc ttgatggcgt ggcgcgccac ggggacgccc
      721 acggcgaaag cgacgatgaa gccggccgat gcgtagatga tgccgaagtt gatggcatgg
      781 accacgccca gctccgactc ccagatcgag gcgtaagcga gcgcctgacc aggcccctgg
      841 gtgaagccgt gggttgccag caggccgagg aagctcgata gctcgccgcc cgtcagcacg
      901 ttgtagccaa ccagcgcgcc gaggccaacg agaccctgcg tgaccaggct gaccacccag
      961 accaccgaca tccacatgcc gccagggatg atggaggtgt tcttgccgcg ctctctgctg
     1021 ccgcccgtca ggcagagcga catgaagctg agggtgaaaa aatggaaggt aaacaccgtg
     1081 aagtcactgc tctcgtagcc gaaggaaagt cccgccgaga cgagagcgaa gccgaggatg
     1141 ccccctacca ggctggccgg cacgagcgct ctgcgcagaa gcggcacctt cgcgcggagc
     1201 acggttccgg ccagcagcat gcacaccatg aaggcaaagg cgatcacccc cggaaacgtc
     1261 gaagtcatgt tgaaaccctt ctgttgtgaa acgtcgcccg aggctgcgcg tcaggcctga
     1321 tagacccgtt cgccgccaac ccaggtctcg agcacccgaa cggcgccgag gcgagacggc
     1381 ggaacgctgc ggggatcctc ttccagcacc acgagatccg ccagcttgcc cggctccagg
     1441 gtgccgatct cgtgatccga gtgacactgc caggccgcat cccgggtcat cgcccgcagc
     1501 gcctgctcca cgcccacgca ttcatggggg gcgagcacct cgtcgtcggt ccgccacagc
     1561 cggcgggtca cggcgttctc gatgcagcgc agcggaccca tctcggtgac gggctcgtcg
     1621 gagtgcaggg tccagcgcac gcccttggcc tcgcaggccg cggtccggtc caggagactg
     1681 gctttctcca gcccgaatat ctcgtctcga aaggccttgc cccaccagta gacgtggccg
     1741 atcagaaagc tgggcgagag cccgaggtcg gcgatccgct cgatctgctc gtcgtggagg
     1801 atgctgcagt gctctatgcg gcagcgcgct cgcgccggat cgagccccgc ggcccgggcg
     1861 gcctggaagg catccagcac cctgtcgatg gcaagatcgc cattggcatg cacggcaacc
     1921 ggccagccct gcccgagtct tcgtttcacg gcctcgttga gcgcctcggg ttcgatgtag
     1981 gcgatgccgt gggagtcggc gttcatgaac ggctcccgct gcagcccgct caacccctga
     2041 ttggaaccgt cgctgacgat cttccaggcg ctggcgcgaa cccagctgtc tcccgtgccg
     2101 aacgagacgt cggaggcctc ccagtcggcg gcaagggtat tgaacaggga gtaacgaaag
     2161 cgcgccgtca tccggttgct ttcgcgcagc gcttcgtaaa gagcgagctc cttggcgccc
     2221 tgcatcgcgc cggtgccctg atcgcagatg gtggtgaagc cgacgctgtt ggcatggcgg
     2281 aacacgtcca ggcacccctc gaccagatcg tgctcgcgca gcatggggtt gtgccgcgcc
     2341 accatggcct gggagacgcc gcccttgagg acgccgtccg gggtgccgtc gggcagtcgt
     2401 ccgaagggcg aaccttccgg gtccggcgta tcccgggtga tcccggcgat ctccagcgcg
     2461 agggagttgc agtagccgaa gtgaagcgac gcgttgtaga cgaagatcgg gtgcgttgtg
     2521 ctgacttcgt ccagcatgga ggcggtcagg gtgccaggcc cctcctgcag cgaggggtcg
     2581 aactgtcggc cgatgatcca cgcgccaggg tcggtatcgg ctgcgacttc cttgagcctg
     2641 gccagcgctc cgctcacggt cgggtagcgg aagggcccca cgttctcgaa ccgtctgagc
     2701 atggcgatgg cggagaggtg catgtggggt tcgacgaacc ccggcaggat ggttctgccg
     2761 ttgcagtcca cgcgcacggt gtcgtcaccg acctcgggca cggcatcggc atcggcattg
     2821 ccgacccatt gaatgcgccc gtcggagatc agcagcgtgt cgaactccga gcccgcatcg
     2881 tccatggtca gaaccctggc gttgcagaac agcgtgggca cgggcgccct cggtgcggcc
     2941 ggcacggacc gccccgcctg ggcctcgacg tccagcccca cccggcgcag gagctcgccc
     3001 agagtcgccg cgcagcaggg acagccgtgc gggccgtgct cgtggcaggg accctcgtga
     3061 acgcggtctg atccgtaggt agttgccctg tcgtacacgc gcatctcccc ccgaggccac
     3121 aagcgcatag attacgctat ttgcatggag cccacattgg gaacactgga cccggagcgc
     3181 gcaatgaggc accgtagcaa ccacggtatc gggaggaagc accatgacta ccgggatgac
     3241 ggtgatgatc gtcatgacca cgctggcggt gaccgtcggc gtcgtcgctc tgcgcgtgtc
     3301 gtggcgtatc gtgaaccggc tgtcggaaga gcggccgcag aacgaggagg agcgctgacc
     3361 gctatcccag cgacggccat caggggcccg tcggcgcgat cccccatacc gcaacgagtc
     3421 tgaatcaacg gagggacgac cgtggcaaag cgcgtcgact ggtattacta ccgcaacggc
     3481 tgaaccagct gtaccagagc atccaagttc ttggaggcaa gcaaaataga gatcaaggac
     3541 cgcacgccgg ccagccagaa gctgacggaa ccggacgccc gcgagctgct cggcaaagcg
     3601 tcgaaaatga tcgtcgcgaa gggtaagaaa atcagcgagt tcggcggtgg taaagcggcc
     3661 accgacgacg cggtggcggc catgctcgga ccgaccggca atctgcgtgc ccctacgctg
     3721 aaagtgggca agacgctgct ggtcggcttc aacgaggagg cctacggcgg cgtgttcggc
     3781 gggtaggacc ggcagcggcc ggagagggag gcgaggatga aattcggcat cttctacgag
     3841 ctgcagctgc caaaaccctg ggaagcggac agcgagtacc ggctgttcaa caacgcgctg
     3901 gatcagatc
//
LOCUS       DBNHQR010000588         4729 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1008534, whole genome shotgun sequence.
ACCESSION   DBNHQR010000588 DBNHQR010000000
VERSION     DBNHQR010000588.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4729)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4729)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4729
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1008534"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="AC16VR_13395"
     CDS             <1..805
                     /locus_tag="AC16VR_13395"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HIR6012199.1"
                     /translation="CGPLARSAADLALALGIMAGPMEREAVGWRLDLPGPDIASLKSL
                     RVAIWPNDDQAPVSRETEARVVMVGETLERLGATVSDTARPDFDVRKAHINYRSLLES
                     VMGSAKPMAYFEEAQARAADLDPEDMSDEAVTLRGIVMHHRDWIRHNFRRERLRRAWD
                     EFFAEWDVLVCPQMATPAFPHDHRPFAERTINVDGVEQPYWQQLFWAGMVSNAYLPST
                     VFPTGPGSDGLPIGLQAISGPYRDYRTIEFASLMAEEIGGFAAPPAMRG"
     gene            1078..2019
                     /locus_tag="AC16VR_13400"
     CDS             1078..2019
                     /locus_tag="AC16VR_13400"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0050896 - response to stimulus [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6012200.1"
                     /translation="MKAFEHAIVGLDFNRDCAAAILDRATQLVDPEEIDVVHAFSGND
                     HSPVTAEEAGLMTMCADYGVASVKFVSGESAHALHEQAGKRSDLIIVGSHGRSGLRGI
                     IHSHSNATLHATPCDLLTVHLDDPPQSRSPPYRSVLVAIHLGPVTLPSKCERCSFWPS
                     AQEEDRLNRGHRFMNALKPIKLIPRHSHASEESHYPLCHVPTEVILHARKIAQQSGAE
                     LSLCHVYMGYDVDTQQMERELLDSLGELYEVPEDRRYSVPGSVSGAIHRLASRIGADL
                     VVLGTHGRSGPSLAVGSTANAVLHGATCDQLSVRIGA"
     gene            2217..2921
                     /locus_tag="AC16VR_13405"
     CDS             2217..2921
                     /locus_tag="AC16VR_13405"
                     /inference="COORDINATES: protein motif:HMM:NF015682.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HIR6012201.1"
                     /translation="MTELTADLIAELQRLDTPTVCNALEVVAPERRGYGYTVRPLVCT
                     RPELPPLVAVARTATIRAAHPGHLRGSDAQALRDAYYAYVDEGPKPSVMVIQDMDGDQ
                     RGYGSWWGEVNSNVHKGFGCLGVITDGSVRDIPDIAEGFQMLADRIGPSHAYVHPVDF
                     GRTVNVAGMRVNGGDLIHADQHGAVVIPWEVAAEVAAAADTIARRERVIIEAARQPGF
                     DMASLRDAWGDAADIH"
     gene            complement(2937..4265)
                     /locus_tag="AC16VR_13410"
     CDS             complement(2937..4265)
                     /locus_tag="AC16VR_13410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HIR6012202.1"
                     /translation="MNVQPLSVRERMGYGVGDFGVNLFFIPAMTYLLYFYTDVFGLSA
                     AAAGGVFLVARVVDAVTDPLMGGIAERTSSRWGRLRPHILFGALPLGAIAVLTFTTPD
                     LNDAGKLWWAAVTYVLFGIAYTVVSIPYSALTASITADHHERTLLTTVRMGCAFAGGY
                     VVSVGMLPLVGLFETQAAGFAWAMVIFATFATGLLWLTVANTRERVQPPATQKLTVRD
                     SFRAVFANPPLLVVIVLFTGGMLSFTIRQSVTLYYFKYNLGREDLIALFFGITLPVMM
                     VGLLGVPRLAAAVGKAGGIVVGALVTIVGCAGLYLTPYDSVTWVFVWSCVIALGGTPI
                     AVLGWAMIPDTVEYAQWRMGLRADGAIFGFASFFQKLAKAVGGAGVAGALALAGYVAN
                     VEQSEASLRAIHNMMTLAPALIMLVLIIAARAYRLDAATHRRIVQELSGA"
     gene            complement(4273..>4729)
                     /locus_tag="AC16VR_13415"
     CDS             complement(4273..>4729)
                     /locus_tag="AC16VR_13415"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HIR6012203.1"
                     /translation="WQRFCEALGADDLLAHPDYQTTADRIARKDQLKEAINAITSQRT
                     WADLVGVLNEAGVPCGAINTIGEGFEDPQVRHLGMAKPAPHPDMGSVNLIRSPINLSR
                     FPQEAAFRNAAPDPGFDTRAVLTELGIPSSRIDALAAAGAIACTDPASA"
BASE COUNT          886 a         1581 c         1526 g          736 t
ORIGIN      
        1 gtgcgggccg ctcgcgcgca gcgccgcgga tctggccctg gcgctgggga tcatggcggg
       61 gcccatggag cgggaggcgg tcggctggag gctcgacctg cccgggcccg acatcgcgtc
      121 gctcaaatcc ctgcgcgtgg ccatctggcc caacgacgac caggctcccg tctcgagaga
      181 aaccgaggcc agagtcgtca tggtgggcga gacgctcgag cggctcggcg ccaccgtctc
      241 ggacaccgcg cgtccggatt tcgatgtgcg caaggcccac atcaactacc gcagcctgct
      301 cgaatccgtg atggggagcg ccaagcccat ggcttacttc gaggaggccc aggccagggc
      361 agcggacctg gatcccgaag acatgtccga cgaggcagtg accttgcggg gcatcgtcat
      421 gcaccaccgg gactggatcc gtcacaactt ccgccgcgag cgcctgcgcc gggcgtggga
      481 cgagttcttc gccgagtggg acgtcctcgt ctgcccgcag atggccaccc cggcatttcc
      541 ccacgaccac cggcctttcg cggagcggac gatcaacgta gacggcgtcg agcagcccta
      601 ctggcagcag ctcttctggg cgggcatggt cagcaacgcc tacctgccga gcacggtgtt
      661 tcccaccggg cccggcagcg acgggctgcc aatcggcctg caggccatca gcgggccgta
      721 ccgcgactat cggaccatcg agttcgcgag cctgatggcc gaggagatcg gcgggttcgc
      781 agccccgccc gccatgagag gatgagttgg gggcgtaagg cgccccatat cctcgaccac
      841 ccgctgctgc cagaaccgcg aatggccgtg gccccgcatg gtgagcccat gcgaaccgtt
      901 ctcgcccgct tctgatcagg cagcgatact ccactgaaac cttcagattt cctaatcgaa
      961 aatcattctt atattcatcg ggctaattag aaaaccgggg gttattccaa aggggtcgtt
     1021 cagaccatgg cttgagggtg ttagactgtt tgatactacc caaacggacg cgttggcatg
     1081 aaagcctttg aacacgcgat agtcgggtta gatttcaacc gcgattgcgc ggcggcgatc
     1141 cttgatcggg ccactcagct ggtcgaccct gaagagatcg acgtcgtgca cgccttctcc
     1201 gggaatgatc actctcccgt cactgccgag gaggccggac tgatgacgat gtgcgccgat
     1261 tacggtgtcg cttccgtcaa gttcgtcagc ggcgaatctg cccacgcact tcacgagcaa
     1321 gccgggaaac gaagcgatct aatcattgtc ggctcccacg gccggtccgg gctgcggggc
     1381 atcattcact cccattccaa cgccacgctg cacgcaaccc cctgcgatct gctgacggtg
     1441 cacctggacg atccgcccca gagtcgctcg ccaccgtatc gaagcgttct cgtcgcgatt
     1501 cacctgggcc cggtcacgct tccttccaaa tgcgagcgat gcagtttttg gccgagcgcg
     1561 caagaggagg atcgactgaa ccgaggccac cggttcatga atgccctcaa acccataaag
     1621 ctgatcccgc gccattcaca cgccagcgaa gagtcgcact accctctgtg ccacgttcca
     1681 accgaggtaa ttctgcacgc ccgcaagatt gctcagcaaa gcggagccga gctctctctg
     1741 tgccacgtgt acatgggcta cgacgtcgat acccaacaaa tggaacgcga gctgctcgac
     1801 agcctcggtg agctctacga ggttcccgag gataggcggt actcggtgcc cggatcggtc
     1861 tcaggtgcaa ttcaccggct ggccagtcgc atcggagcag atctcgtcgt gctcggcacg
     1921 cacggacgct caggcccgag tcttgcggtg ggttcgaccg ccaatgcggt cctgcacggc
     1981 gcaacgtgcg atcagctgtc ggtgcgaatc ggcgcctgac acccggcacg tcggcgggca
     2041 cctcgtcagg ccgggctcag tcggcgaact tgcgcacgtt gattaaccgg acgataagac
     2101 gataaacagc attccgttcg caacgtgcac gccggtgaaa gagctccgac gcagcgcttg
     2161 gcccatccgc gcgcgggcgt gatactttca gcgtatggaa ccgtcaggaa gagcccatga
     2221 ccgaactcac cgcggacctc atcgccgagc ttcagcgtct ggatacgccc accgtctgca
     2281 acgcgctgga ggtggttgcc ccggagcggc gcggctacgg ctacaccgtt cgcccccttg
     2341 tctgcacccg cccggagctg ccgcccctgg tggcggtggc gcgcacggcc accatccgtg
     2401 ccgcgcaccc cggacacctg cgcggcagcg atgcacaggc actgcgtgac gcctactacg
     2461 cctacgtgga cgaggggccc aagcccagcg tcatggtcat ccaggacatg gacggcgacc
     2521 agcgcggcta cggctcctgg tggggcgagg tgaacagcaa cgtgcacaag gggttcggct
     2581 gcctcggcgt gatcaccgat ggcagcgtgc gcgacatccc ggacatcgcc gaaggttttc
     2641 agatgctggc cgaccgcatc ggcccgtcac acgcctacgt tcacccggtg gacttcggcc
     2701 gcacggtgaa cgtggccggc atgcgcgtga acggcggcga cctgattcac gccgaccagc
     2761 acggcgcggt ggtgatcccc tgggaagtcg cagccgaggt cgccgcggcg gcggacacca
     2821 tcgcgcgccg cgagcgggtc atcatcgagg cagcccgaca gcccggcttc gacatggcga
     2881 gcctgcgcga cgcctggggg gacgcggcgg acattcacta gaattacccg ggcgcgtcac
     2941 gcgccggaga gctcctgcac gattcgccga tgagtcgcgg cgtccaggcg gtaggcccgg
     3001 gcggcgatga tcagcaccag catgatgagc gccggcgcca gggtcatcat gttgtgaatg
     3061 gctctgagcg acgcctcgga ctgctccacg ttggccacgt agccggccag ggccagagct
     3121 cccgcgacgc ctgctccgcc cacggccttg gccagcttct gaaagaagct cgcgaaaccg
     3181 aagatggctc catcggctct gagccccatg cgccattggg cgtactccac cgtgtccggg
     3241 atcatggccc agcccagcac ggcgatgggc gtcccgccaa gggcgatgac gcacgaccag
     3301 acgaacaccc aggtcaccga gtcgtagggc gtcaggtaca gacccgcgca tccgacgata
     3361 gtgacaagcg cccccacgac gatgccgccc gctttgccaa cggcggccgc cagccggggc
     3421 accccgagca ggccgaccat catcacggga agagtgatgc cgaagaaaag cgcgatgaga
     3481 tcctcccggc cgaggttgta cttgaagtag tagagggtga ccgactggcg gatggtgaaa
     3541 gagagcatgc cgccggtgaa aagcacgatc acgacgagca gcggcgggtt tgcgaacacg
     3601 gccctgaagc tgtcacgaac cgtcagcttc tgggtagccg gcggctgcac gcgctcgcgc
     3661 gtgttcgcca cggtcagcca cagcagcccc gtggcaaacg tagcgaagat caccatggcc
     3721 caggcaaaac ccgcggcttg ggtctcgaaa agacccacca ggggcagcat gcccacggag
     3781 accacgtagc cgccagcgaa cgcgcaaccc atgcgcacgg tggtcagcag cgtccgctcg
     3841 tgatgatcgg ccgtgatcga cgcggtcagc gccgagtagg gaatggagac caccgtgtag
     3901 gcgatgccga acagcacgta cgtgacggcc gcccaccaga gcttgccggc atcgttcagg
     3961 tccggggtgg tgaaggtgag cacggcgatc gcccccagag gcagcgcgcc gaagagaatg
     4021 tggggccgaa gccgccccca gcgactgctc gtgcgctcgg caatcccgcc catgagcggg
     4081 tcggtcaccg cgtccaccac ccgggccacg aggaacacgc ctccggcggc cgctgcgctc
     4141 agcccgaaca cgtccgtgta gaagtacagc aggtacgtca tggccgggat gaagaacaga
     4201 ttgacgccga aatcgcccac gccgtagccc atgcgctccc gaaccgaaag cggttggacg
     4261 ttcacggcgg cttcaggccg atgccgggtc ggtgcaggcg atggcgcccg ccgccgccag
     4321 cgcgtcgatg cgggagctgg gaataccgag ctcggtcagc acggcgcggg tgtcaaaacc
     4381 ggggtccgga gccgcgttgc ggaaggccgc ctcctgagga aagcgcgaca ggttgatggg
     4441 ggagcggatg aggttcaccg aacccatatc agggtgcggc gccggcttcg ccatgcccag
     4501 gtggcgcacc tgaggatcct cgaaaccttc gccaatggtg ttgatggccc cgcacggcac
     4561 gcccgcctcg ttcaggacgc ccaccagatc ggcccacgtg cgctggcttg tgatggcgtt
     4621 gatcgcctcc ttgagctggt ccttgcgcgc gatgcgatcc gccgtggtct gataatcggg
     4681 atgggccagc agatcgtccg cccccagcgc ctcgcagaac cgctgccac
//
LOCUS       DBNHQR010000589         4144 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1450576, whole genome shotgun sequence.
ACCESSION   DBNHQR010000589 DBNHQR010000000
VERSION     DBNHQR010000589.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4144)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4144)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4144
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1450576"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="AC16VR_13420"
     CDS             complement(<1..411)
                     /locus_tag="AC16VR_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012204.1"
                     /translation="MSFLPAGFHVPELLVTPTFSARPLTVDDVVRDYDAVMSSRERLV
                     GTFGSESSWPAADLSLKQNLIDLGWHQKEFQKRRSFAYTLVDPDDTLTIGCFYVYPEA
                     PDGFDASVYLWVRTSHSDMDEAVFSAVKQWLEEAW"
     gene            complement(416..739)
                     /locus_tag="AC16VR_13425"
     CDS             complement(416..739)
                     /locus_tag="AC16VR_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012205.1"
                     /translation="MRAVAIIIAAALLAVSPVVTGDRSAAGIRVSESQRYCQAARQCT
                     KVQTRCGGCDCGTAINESFAAEHRDRLAALCGSHEQVECERSCPETRPICVIGVCALE
                     PLRGS"
     gene            complement(747..950)
                     /locus_tag="AC16VR_13430"
     CDS             complement(747..950)
                     /locus_tag="AC16VR_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012206.1"
                     /translation="MNEIHLLLGLVLSAIGIGYLVYGRRQQAPVFFIAGLGLVSIPFF
                     VGSTSTLLVAGAVVGAAPFLIRL"
     gene            complement(1033..1434)
                     /locus_tag="AC16VR_13435"
     CDS             complement(1033..1434)
                     /locus_tag="AC16VR_13435"
                     /inference="COORDINATES: protein motif:HMM:NF016152.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HIR6012207.1"
                     /translation="MTAEHPPMDGEAARAHLLARPEAREDFPFGPDVYVYKIGPKMFA
                     TLSYVNGVAQMNLKCEPNEAMALRDIFEAVRPGYHMNKAHWNTVTLDGSIPAGELERM
                     MDRSYALVVRGLRKAERTGLEIRHGKEAVYR"
     gene            complement(1431..1613)
                     /locus_tag="AC16VR_13440"
     CDS             complement(1431..1613)
                     /locus_tag="AC16VR_13440"
                     /inference="COORDINATES: protein motif:HMM:NF022662.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3012 domain-containing protein"
                     /protein_id="HIR6012208.1"
                     /translation="MHIQTKLSIIVLAAALAACSPEVGSDAWCEDMAEKDKGDWTANE
                     AAEFARSCLFKSYDDE"
     gene            1927..2199
                     /locus_tag="AC16VR_13445"
     CDS             1927..2199
                     /locus_tag="AC16VR_13445"
                     /inference="COORDINATES: protein motif:HMM:NF012305.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA recognition motif domain-containing protein"
                     /protein_id="HIR6012209.1"
                     /translation="MRTKVNMKLFVGNLPWSVTDQDLEEMFAPFGAVGSAKVITDRDT
                     GRSRGFGFVEMSDDSARNAIADLNESEISGRKLTVNEARDRDRGRR"
     gene            complement(2327..2617)
                     /locus_tag="AC16VR_13450"
     CDS             complement(2327..2617)
                     /locus_tag="AC16VR_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012210.1"
                     /translation="MRAIRGRALIVSAAIGLSACASDPRVEDLGGGRISVDCSGGTPS
                     WTRCQALATEACAGRGFDVVSQVSNEGSANVGRNDWSTEGSQVTRRMIVACR"
     gene            complement(2614..2916)
                     /locus_tag="AC16VR_13455"
     CDS             complement(2614..2916)
                     /locus_tag="AC16VR_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012211.1"
                     /translation="MIDAFNYFVFVLVPIFLLGFQAVLLAVLFFLKARKWLSVYAFAS
                     GGFVAGLGFGYLFATLSGSTQTLLVVCLSVGAFGILSGIFWWFLLVKKASQAQEQA"
     gene            complement(2965..3309)
                     /locus_tag="AC16VR_13460"
     CDS             complement(2965..3309)
                     /locus_tag="AC16VR_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012212.1"
                     /translation="MNLDLTLKAAAILLLLISGMEYLVSSVIHLGPVLFANVMFVTSI
                     FSRTQPRRIAVIALAFAVVIPIGAFRAYVRDDSALWVALLNLAIFCYVAFVAVQTLTA
                     PSGRNIGNGQGS"
     gene            complement(3425..3964)
                     /locus_tag="AC16VR_13465"
     CDS             complement(3425..3964)
                     /locus_tag="AC16VR_13465"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR6012213.1"
                     /translation="MRQISSIPFDIFSVRFKARLALLALGPLLGSAPAALADLKIDRL
                     VIEKSQRVLILEHDGAPVRSYSIALGGAPRGHKQREGDQRTPEGQYTIDARNEDSAFH
                     RSLRISYPNEADRRRAAMEGVNPGGQIMIHGIGEADPEMRKLHPYIDWTAGCVAVTDS
                     EIEEIWALVEIGTPIVIRP"
BASE COUNT          790 a         1277 c         1340 g          737 t
ORIGIN      
        1 ccaggcctcc tccagccact gcttcacagc agagaaaacc gcctcgtcca tgtctgagtg
       61 gctggtgcgc acccacaggt agacggaggc atcgaatccg tccggcgcct cgggataaac
      121 gtagaagcag ccgatggtca gggtgtcgtc cgggtcgacg agcgtgtagg cgaacgagcg
      181 gcgcttctgg aactccttct ggtgccagcc gaggtcgatg agattctgct tgagggaaag
      241 atcggcggcg ggccaactcg attccgagcc gaacgtgccg accagccgtt cacggctgct
      301 catcaccgcg tcgtagtcgc gaaccacgtc gtccacggtc agcggtctgg cgctgaaggt
      361 gggcgtgacc agaagttccg gaacgtgaaa acccgccggc agaaagctca cgagactatg
      421 atcccctcag aggctccagg gcgcacaccc cgataacaca gatcggccgg gtttcagggc
      481 acgaccgctc gcattctacc tgctcatgac tgccgcacag cgcggccagc cgatcgcggt
      541 gctcggcggc aaacgactcg ttgatggcgg tgccgcaatc gcagccgccg cagcgggtct
      601 ggaccttggt gcattggcgt gccgcctggc agtagcgttg ggattcgctg acgcgaatgc
      661 cggcagccga tcggtcaccg gtcaccacgg gtgagaccgc gagcagcgca gcggcgatga
      721 tgattgcaac ggccctcatg gcgggctcac agcctgatca ggaaaggcgc cgcaccgacg
      781 acggccccgg cgaccagaag cgttgacgtg gagccgacga aaaatgggat cgaaacgagc
      841 cccagaccgg caatgaaaaa aacgggcgcc tgctggcggc gtccgtatac cagatagcct
      901 atgccgatgg cgctgagcac caggcccaag agaaggtgaa tttcgttcat ccgctcctca
      961 gcgattgctg gcgggaggac tataacagcg tgctgaacac ctttgcagac gccccgggat
     1021 cgatgcctga agctaacggt agacggcctc tttgccgtgg cgtatctcca gccccgttct
     1081 ctctgctttt ctgaggcctc ggacgaccag agcgtatgat cggtccatca tgcgctccag
     1141 ctcgcccgca ggaatggatc cgtcgagcgt gaccgtgttc cagtgggcct tgttcatgtg
     1201 ataaccgggc cgaacggcct cgaagatgtc ccgcagggcc attgcctcgt tgggttcgca
     1261 tttcaggttc atctgggcga cgccgttcac gtagctcagg gttgcgaaca tcttagggcc
     1321 gatcttgtag acgtagacat ccggtccgaa cgggaagtct tcccgcgcct cgggccttgc
     1381 caggaggtgg gcgcgcgcgg cttccccgtc catgggcgga tgctctgcgg tcactcgtcg
     1441 tcgtaggatt tgaacaggca gctgcgggcg aattccgcgg cctcgttggc agtccaatcg
     1501 cccttgtcct tctcggccat gtcttcgcac cacgcgtcac ttcccacctc gggcgagcaa
     1561 gctgcgagcg ctgcggcgag aacgatgatc gatagcttcg tttgaatgtg cattgtgctc
     1621 tccggcccag cgggcgctcg gggtttcaag gatcgttcag ctggctcgtg gggcgccgtt
     1681 gctagggacg ctgcggcgac cgtggcaagc ggcccgcgag gcccccgcaa atgaccacct
     1741 ggcgggtttc ctgcgccccg gcagcctgtt gtatatgcag gaaaaacgag ttactttctc
     1801 gtcaccggtc gggcctcgag tttaacgcct ctctgagctg ccttcgataa gtctaattca
     1861 gcaagcggtg cgggtgcctg atagtgcaga ttgcgcccgc cggtctgggg gatgagaacg
     1921 taccgcttgc gcactaaggt gaatatgaag ctatttgttg gaaatctgcc ctggagcgta
     1981 acagaccagg atctcgaaga aatgttcgcg ccgttcggtg ccgttggctc tgccaaggtg
     2041 atcaccgacc gcgacactgg tcgatcgcgt ggtttcggtt tcgtcgagat gagtgacgat
     2101 tcggcgcgca acgccatcgc agatctgaac gagtccgaga tatcgggccg caagctcacg
     2161 gtcaacgaag cccgcgatcg cgatcgcggg cgccggtagc cgcaccggaa cacgtcagcc
     2221 tgtcagtcaa acgcgcgacg ggtcccttta gggccgtcgc cgttggcggt gggctggctt
     2281 cagttgacag gcacgtcggc cgcccgatgg ccgtggcgga ggctcgtcat cggcacgcga
     2341 cgatcatacg acgagtgacc tgcgagcctt cggtcgacca atcgttgcgg ccgacgttcg
     2401 cgctcccctc gttgctcacc tgggagacca cgtcgaagcc gcgcccggcg caggcctcgg
     2461 tggccagcgc ctggcagcgc gtccacgacg gggtgccgcc ggagcaatcc accgagatgc
     2521 gcccgccgcc gagatcttcc acgcgggggt ccgacgcgca ggcgctgagc ccgatcgcgg
     2581 ccgagacgat cagggcgcga ccacggatgg ccctcatgcc tgttcctgag cctgcgaagc
     2641 cttcttgacc agaagaaacc accaaaagat gcctgacaag attccaaacg cgcctacgga
     2701 caggcacacg accagcagcg tctgcgtcga tccgctcagc gtggcgaaga ggtagccgaa
     2761 ccccaggccg gcgacgaagc cgccgctggc gaacgcgtaa accgagagcc actttcttgc
     2821 cttgagaaag aagaggacgg cgagcagcac ggcctggaaa cccagcagaa atatgggtac
     2881 cagaacgaat acgaaatagt tgaacgcgtc gatcacgtcc ggtctccagt ctttgcttgt
     2941 cggaaggcta cactggccgg gcccctacga accttgcccg ttcccgatgt tcctgcccga
     3001 aggcgccgtc agcgtttgca cggcgacgaa ggcaacgtaa cagaaaatcg ccaggttgag
     3061 cagggcgacc cacagggcgg agtcgtccct gacgtaggcc cggaacgcgc cgataggaat
     3121 gacgacggca aacgcgagag cgatgacggc gattcgccga ggttgggttc ggctgaaaat
     3181 gctggtgacg aacatcacgt tggcaaacag cacgggaccg aggtggatga ccgacgagac
     3241 caggtactcc atgccggaga tcagcagcag cagtatggcg gcagctttca gggtgagatc
     3301 gagattcaag atgaaggatc ctcgaggccg tcgatcgggc gccgtccggg ttgaggggcg
     3361 agctcggctg atgacgtggc ggcgcggttc atctgcagtg cgtcgagtcc ggcggggcgc
     3421 gtcgtcaggg ccgaatgacg atgggggtac cgatctcgac cagggcccag atctcctcta
     3481 tctcgctgtc ggtcaccgcg acgcagccgg cggtccagtc gatgtagggg tgcagtttgc
     3541 gcatttccgg atcggcctcg ccgatgccgt ggatcatgat ctgaccaccg ggattgaccc
     3601 cctccatcgc cgctcgccgc cggtcggcct cgttcgggta ggagatccgc agagagcggt
     3661 ggaatgcgct gtcctcgttg cgggcatcga tggtgtattg cccctccggc gtgcgctggt
     3721 cgccctcgcg ctgcttgtgc ccccgcggcg ccccgcccag cgcgatcgag taggatcgca
     3781 cgggtgcccc gtcgtgctcg aggatcagca ctcgctgaga cttctcgatc accagccggt
     3841 caatcttgag atccgcaagc gctgccggtg ccgacccgag cagcgggccc agcgccagaa
     3901 gcgccagtcg ggctttaaag cgaacggaaa agatgtcgaa agggatcgag gaaatttgcc
     3961 tcatgcccgt accatgaccg cgtgacctct cgagcccgaa gatagcacgt gaattctcac
     4021 ctgcaacggt cagtttgtat ccgggttacg gctcgggtgg cggcacctga tcgaaggtta
     4081 tccgaaccag catgtggtct tcagggtcgg cgatgacctc gagcgcctgc aaggcatccc
     4141 gata
//
LOCUS       DBNHQR010000590         3085 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1703955, whole genome shotgun sequence.
ACCESSION   DBNHQR010000590 DBNHQR010000000
VERSION     DBNHQR010000590.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1703955"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="AC16VR_13470"
     CDS             <1..447
                     /locus_tag="AC16VR_13470"
                     /inference="COORDINATES: protein motif:HMM:NF024675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HIR6012214.1"
                     /translation="EDGWIDYNGHMNMAFYNLAFDRALDHVYDRLGIGADYVRLRGGS
                     CFTREVHVNYLRELSAGDPIRVTFQLLDWDAKRLHFFEEMYHADQGYLAATSEQLALH
                     VSMTSRSTHPFPQDIQAHLEELMTAHRPLPTPAQVGRVMGIRRSPR"
     gene            complement(463..2136)
                     /locus_tag="AC16VR_13475"
     CDS             complement(463..2136)
                     /locus_tag="AC16VR_13475"
                     /inference="COORDINATES: protein motif:HMM:NF027688.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcineurin-like phosphoesterase C-terminal
                     domain-containing protein"
                     /protein_id="HIR6012215.1"
                     /translation="MRLCCCVALALLGLGWSSFGLGSGTANGKVFEDLDGDGVWEPGE
                     PGVKGVRVSNGIAVVLTGEDGGYTLPVPEEAVIFITKPSGYATPVNAHQLPQFYYVHQ
                     PDGSPDHLRYPGVDPTGPLPERIDFPLRRQDEPARFQAILFADTQPQTEEELDFIRDD
                     VIAELIGTGARFGMTMGDILFDELSLFPRYNALIGTLGIPWYNVPGNHEMNFLAADDR
                     HALETFKRYFGPPYYAFEYADALFVVLDNIEYLGNGESDPADVRGTGGYVARIGRRQL
                     DWLAAELRHVPVDKLVFLGMHAPLATYLDDSPRNNTADRRELFGLLKGRPNLYAVAGH
                     THTTEHHYFGRDEGFAGPGELHHHVLSTVSGAWWSGPFDTRGVPTSDQRDGTPNGYHV
                     LEVDGTKVTVRYKAASAPADYQMRIMFDVVHHGLRKDAMRDFRHGQLFDGRFSVDQVP
                     AADILVNLFDGGPRSEVAFRIGDGEYLPMRRVRRADPYLIELHARNAETKKSWFKAEP
                     SSHIFVADVPDDLGPGVYPVGVRAIDEFGRAHHAHAVLEITGGSSPSGL"
     gene            complement(2149..>3085)
                     /locus_tag="AC16VR_13480"
     CDS             complement(2149..>3085)
                     /locus_tag="AC16VR_13480"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6012216.1"
                     /translation="KGLGVVTEETGRTLAASPLFASVWVCGTLVNLGGSEEQKGELLP
                     LISTGELLMSLALEESHRHDPYGLETRAAAADGGFVLNGRKTFVLDGHVADKLVVAAR
                     TSGERGDRNGITLFVVDSDAEGLSVTRTVMVDSRNAANVELDGVRVGADAVLGAVGGA
                     AEVLDPALDIARIGLCAEMLGSLQECFERTVEYLKEREQFGVPIGSFQALKHRAANMF
                     CEVELAKSCVLEALTALDENRDPQEIARLASLAKAKVGETFHLVSREGIQMHGGIGMT
                     DEFDIGFFIKRAAVTEQTFGDVGFHRNRYGELEGY"
BASE COUNT          562 a         1032 c          975 g          516 t
ORIGIN      
        1 gaggatggct ggatcgacta caacggccac atgaacatgg ccttctacaa cctcgccttc
       61 gaccgggcct tggatcacgt ctacgaccgg ctgggcatcg gcgcagacta cgtacggctg
      121 cgcggcggct cctgcttcac cagggaagtc cacgtgaact acctgcgcga gctaagcgcc
      181 ggcgacccga tccgggtgac gtttcagctg ctggactggg acgctaagcg gctgcacttt
      241 ttcgaagaga tgtaccacgc agaccagggc taccttgccg ctacctcgga gcagctggcg
      301 cttcacgtga gcatgacaag ccgatcaacg cacccctttc cccaggacat ccaggcgcat
      361 ctcgaggaac tcatgacggc acatcggccg cttccgacgc cggctcaggt cggacgggtc
      421 atgggcatca gacgcagccc ccgctgaaac ggcgcaggaa aatcagaggc ccgacggcga
      481 gctgccgccg gtgatttcca gcaccgcatg cgcgtggtgc gcgcgaccga actcgtcgat
      541 cgcacgcacg cccaccgggt agacgcccgg accgagatcg tcgggaacgt ccgcaacgaa
      601 gatatgcgac gacggctcgg ccttgaacca ggacttcttc gtctcggcgt tgcgcgcgtg
      661 cagctcgatc agatagggat ccgcgcgcct cacccggcgc atcggcaggt actccccgtc
      721 accgatgcgg aatgccacct cggagcgcgg acctccatcg aagaggttga ccaggatgtc
      781 tgcggcgggg acctgatcca cggagaaacg accatcgaac agctgcccgt ggcggaagtc
      841 acgcatggcg tccttgcgca gaccgtggtg caccacgtcg aacatgatgc gcatctggta
      901 gtcggcgggc gcgctggcag ccttgtagcg cacggtcacc ttcgtgccgt ccacctccag
      961 gacatggtac ccgttggggg ttccgtcgcg ctgatccgac gtgggcacgc cgcgggtatc
     1021 gaacggcccg ctccaccacg ccccggaaac cgtgctgagc acgtgatggt ggagctcacc
     1081 gggcccggca aatccttcgt ccctgccgaa gtagtgatgc tcggtggtgt gcgtgtggcc
     1141 ggcgacggcg tacagattgg gccggccttt gagcaatccg aagagctcac gccggtcggc
     1201 ggtattgttt ctcgggctgt catcgaggta ggtggcaagc ggggcgtgca tgccgaggaa
     1261 aaccagcttg tcgacgggca cgtggcgcag ctctgccgcc agccagtcga gctgccgcct
     1321 gccgatcctg gcaacgtagc cgccggttcc ccggacgtcg gccggatcgc tttcgccgtt
     1381 accgagatac tcgatgttgt ccagaaccac gaagagcgca tccgcatact caaaggcgta
     1441 gtaaggcgga ccgaaatacc gcttgaacgt ctcaagcgcg tggcgatcgt cagccgccag
     1501 gaaattcatc tcgtgattgc ccggcacgtt gtaccagggt atgcccaggg tcccgatgag
     1561 cgcgttgtag cgcggaaaaa gcgagagctc gtcgaacagg atgtcaccca tggtcatacc
     1621 gaagcgcgcg ccggtgccga tgagctccgc gatcacgtcg tcccggatga aatccagctc
     1681 ctcctcggtc tggggctggg tgtcggcgaa caggatggcc tggaaacggg cgggttcatc
     1741 ctgccggcgc agcgggaaat cgatacgctc gggcaacggg ccggtgggat ccaccccggg
     1801 gtagcgcaga tggtcgggcg agccgtccgg ctggtggacg tagtagaact gcggcagctg
     1861 gtgagcgttc accggcgttg cgtaaccgct gggcttggtg atgaagatca cggcttcctc
     1921 gggaaccggc agcgtgtagc cgccgtcctc gccggtcagc accacggcga tgccgttgga
     1981 gaccctcaca cccttcacac caggctcgcc gggttcccag acgccgtcgc cgtcaagatc
     2041 ctcgaagact tttccgttcg ccgttccgga gccgagcccg aagctcgacc agcccagacc
     2101 gagcagggcc agcgcgacgc agcagcacag cctcaacgtc ccgggcccct agtaaccttc
     2161 cagctctccg tatcggttcc ggtggaagcc cacgtcaccg aaagtctgct cggtcaccgc
     2221 cgctcgcttg atgaaaaacc cgatgtcgaa ctcgtcggtc atcccgatgc cgccatgcat
     2281 ctgtatgcct tcccgggaaa ccaggtgaaa cgtctcgccc actttcgctt tcgcgaggct
     2341 agccagccgg gcgatttcct ggggatcgcg gttctcgtca agcgccgtca gggcttcgag
     2401 cacacaggat ttggcaagct cgacctcgca gaacatgttt gccgcgcggt gcttgagggc
     2461 ctgaaaggaa ccgatgggca cgccgaactg ctcgcgctcc ttgagatact cgaccgtgcg
     2521 ctcgaagcac tcctggaggc tgcccagcat ctcggcgcag aggccgatgc gggcaatgtc
     2581 gagcgccggg tccagaacct cggccgcgcc gccgacggcg ccgagcaccg catcggcgcc
     2641 gacccgaacg ccgtccagct cgacgttcgc agcgttgcgc gagtccacca tgacggtgcg
     2701 tgtcaccgac agcccttcgg cgtcggaatc cacgacaaac agcgtgatcc cgttccgatc
     2761 cccccgctca cccgaagtcc gggcggcgac cacgagcttg tcggccacgt gcccgtcgag
     2821 cacgaaggtc ttgcgcccgt tcagtacgaa cccgccgtct gcggcggcgg ccctggtttc
     2881 gagcccgtaa gggtcatgac ggtgagactc ctcgagcgcg aggctcatca gcagctcacc
     2941 ggtggagatc agcggcagca gctcgccctt ctgctcctcg ctgccaccca gattcacgag
     3001 ggtgccacac acccagacgc tggcaaaaag cgggctggcc gccagggtcc gaccggtttc
     3061 ttcggtcacc acgcccaggc ccttg
//
LOCUS       DBNHQR010000591         2053 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1513812, whole genome shotgun sequence.
ACCESSION   DBNHQR010000591 DBNHQR010000000
VERSION     DBNHQR010000591.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1513812"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /gene="sucC"
                     /locus_tag="AC16VR_13485"
     CDS             <1..390
                     /gene="sucC"
                     /locus_tag="AC16VR_13485"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701527.1"
                     /note="catalyzes the interconversion of succinyl-CoA and
                     succinate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit beta"
                     /protein_id="HIR6012217.1"
                     /translation="CMVNGAGLAMGTMDLVKIHGGNPANFLDVGGGATKEAVSEAFKI
                     ILSDENVKAVLINIFGGIVSCAIIAEGIVGAVQEVGVEVPVVVRFEGNNAPRGREVLA
                     ASGLNIIAAESLADAARKAVAAAGGRP"
     gene            387..1262
                     /gene="sucD"
                     /locus_tag="AC16VR_13490"
     CDS             387..1262
                     /gene="sucD"
                     /locus_tag="AC16VR_13490"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009619384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HIR6012218.1"
                     /translation="MSILVDSSTRVICQGFTGAQGTLHSEQAIAYGTQVVGGVTPGKG
                     GSEHLGRPVFNTVADAVEATGATASVIYVPAPFCKDSILEALDAGIELVVCITEGIPT
                     LDMLAVKAKLDTTSSRVIGPNCPGVITPGECKIGIMPGEIHLPGRVGIVSRSGTLTYE
                     AVKQTTDRGFGQSSCVGIGGDPIPGSHFIDILALFEADPATEAIVMVGEIGGTAEEEA
                     AEFIRSNVSKPVVSYIAGVTAPPGKRMGHAGAIIAGGKGTAAEKFAALEAAGVRTVRS
                     LAEIGDAIAEVTGWS"
     gene            1320..>2053
                     /locus_tag="AC16VR_13495"
     CDS             1320..>2053
                     /locus_tag="AC16VR_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6012219.1"
                     /translation="MSEQPAQETFGFQTEAKQLLKLMIHSLYSNREIFLRELISNASD
                     AIDKLRFRSISDPSLAGDDAEFAITLSFDEDAGTVTVADNGIGMTRDEVIEHLGTIAK
                     SGTAEFFAQLSGDQKKDAALIGQFGVGFYSAFIVADEVEVLTRGAGTEQGTRWVSKGE
                     EDFSVADAPDVPRGTRVTLHLKEDAKEFADSFRLQSVVRRYSDHIGVPVRMPKVGDGE
                     DAGPEYETVNSAKALWTRSRSEVEDD"
BASE COUNT          394 a          617 c          688 g          354 t
ORIGIN      
        1 tgcatggtga acggcgcggg cctggccatg ggcaccatgg atctggtgaa gattcatggc
       61 ggcaacccgg ccaacttcct cgacgttggc ggcggcgcga ccaaggaggc agtttcggag
      121 gccttcaaga tcattctctc ggacgagaat gtgaaggccg tcctgatcaa catattcggc
      181 ggcatcgtct cgtgcgccat catcgccgaa gggatcgtgg gagccgtcca ggaagtgggg
      241 gtcgaggtgc ccgtggtggt gcgtttcgag ggcaacaacg ccccgcgggg acgcgaggtg
      301 ctcgccgcca gcggtttgaa catcatcgct gcggagagtc tggccgacgc cgcccggaaa
      361 gccgtggcag cggcaggagg acggccatga gcattctggt cgacagcagc acaagggtca
      421 tctgtcaggg cttcacgggc gctcagggca ccctgcacag cgagcaggcc attgcctacg
      481 gcactcaggt agtcggcggc gtcacgcccg gcaaaggtgg ttccgagcac cttggacgac
      541 cggtgttcaa tacggtggcg gatgccgtgg aagccaccgg cgccaccgcg tcggtgatct
      601 acgtgcccgc tccgttctgc aaggactcca tcctcgaggc gctggacgcc ggcatcgagc
      661 tcgtggtctg catcaccgaa ggcataccga ccctggacat gctcgcggtg aaagccaagc
      721 tcgacacgac gagcagccgc gtcatcgggc ccaactgccc tggagtcatc acgccgggcg
      781 agtgcaagat cggcatcatg ccgggcgaga tccatctgcc gggccgcgtg ggcatcgtgt
      841 cccgctcggg aaccctcact tacgaagcgg taaagcagac tacggaccgc ggattcggcc
      901 agagcagctg cgtcggcatc ggcggtgatc ccattcctgg gagccacttc atcgacatcc
      961 tggcgctgtt cgaagcagac ccggcgaccg aagccatcgt gatggtgggg gagatcggtg
     1021 gaacggcgga ggaggaagcg gccgagttca ttcgatcgaa tgtctccaaa cccgtggttt
     1081 cctacatcgc cggggttacc gccccgccag gcaagcgtat gggtcatgcc ggggcgatca
     1141 tcgccggagg caagggcacg gcggccgaga aatttgcggc tcttgaggct gcgggcgtgc
     1201 gcacggtgcg aagcctggca gaaattggcg acgccattgc cgaggtgacc ggctggtctt
     1261 gaaattccgg ctttagcagc catatagatg ccaacgcggg gatcacgtga cgggtagcca
     1321 tgtctgagca accagcccag gagaccttcg gcttccagac ggaagccaag caactgctca
     1381 agctgatgat tcactctctg tactcgaaca gggaaatctt tctgcgcgag ctcatatcca
     1441 acgcctcgga cgccatcgac aagcttcgct tccggtccat ttcggacccc agcctggcag
     1501 gtgacgacgc cgagtttgcc attaccttgt cttttgatga ggacgcgggc accgtgacgg
     1561 ttgccgacaa cggcatcggg atgacgcgcg acgaggtgat cgagcacctc ggcaccattg
     1621 ccaagtccgg tacggcggag ttctttgctc agctcagcgg agatcagaag aaggatgcgg
     1681 ccctgatcgg gcagttcggc gttggtttct acagcgcttt catcgttgcc gacgaggtcg
     1741 aggtgctgac ccgaggcgcc ggcaccgagc agggaacccg ctgggtctcc aaaggcgagg
     1801 aggacttctc cgtcgcggat gcgcctgacg tgccgagagg gacccgcgtg acccttcacc
     1861 tcaaggaaga tgccaaggag ttcgccgatt ccttccggct gcagagcgtc gtccgccggt
     1921 actcggatca tatcggggtt cccgtccgga tgcccaaggt cggtgatgga gaggacgccg
     1981 gcccggagta cgaaaccgtc aactcggcca aggcgctgtg gacccggtcg cgcagcgagg
     2041 tcgaggacga cga
//
LOCUS       DBNHQR010000592         2956 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1642198, whole genome shotgun sequence.
ACCESSION   DBNHQR010000592 DBNHQR010000000
VERSION     DBNHQR010000592.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1642198"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="AC16VR_13500"
     CDS             <1..532
                     /locus_tag="AC16VR_13500"
                     /inference="COORDINATES: protein motif:HMM:NF012568.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR6012220.1"
                     /translation="IAPEVRTSIVGRLADAIGIQGLSAGQERDLHEAQSTTSRAALEK
                     RHHQKTGALFVACLEAGARIAGVDETTMAAMTKFGERVGIGFQILDDLLDLSASESTG
                     KDAHRDEGKATFATVMTIQEAEREAITCLDNALAELADQIADAGPFAVFVDLVVRAYA
                     DQVSRRGSEIAGALRP"
     gene            529..1014
                     /locus_tag="AC16VR_13505"
     CDS             529..1014
                     /locus_tag="AC16VR_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020305761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HIR6012221.1"
                     /translation="MTVVVNAACFLTALAIMEWVAWVTHKYVMHGFLWSLHESHHQPR
                     KGRFERNDWFAVFFAVPSIVLIYLGNNGYPAALWGGLGVAAYGLIYFIFHDVIVHRRL
                     DTGYAPRSRYMRRIVHAHRLHHAVSEKHGAVSFGFVYAPPIPVLRAEMKALAASGASG
                     S"
     gene            complement(1003..1932)
                     /locus_tag="AC16VR_13510"
     CDS             complement(1003..1932)
                     /locus_tag="AC16VR_13510"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014597913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HIR6012222.1"
                     /translation="MSDVFAASASIISRGSKSFAAAARLFDPTTRGHAIMLYAWCRHC
                     DDEIDGQDLGIGQRSPAPPEQRARLEQLRRDTEAAMRGEAVDGTPFVAFQRVFLECDI
                     PRNYPLELLDGFAMDVEGHECRTLSDTLRYCYHVAGTVGGMMATVMGIRDPEVVARAV
                     DLGIAFQLTNIARDVMQDAADGRCYLPRQWLADAGVPEDTGQFPDHRDVIYGQTIRIL
                     DEAERYYESARHGIGRLRFRNAWAIASALAIYRDIGRVVRRDGPDVWQHRATVGVGRK
                     LVLLLKGAALAGLQVTFGRRRTAPIRRGLWSAS"
     gene            complement(1933..>2956)
                     /gene="crtI"
                     /locus_tag="AC16VR_13515"
     CDS             complement(1933..>2956)
                     /gene="crtI"
                     /locus_tag="AC16VR_13515"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007253234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR6012223.1"
                     /translation="VSKFIEDEHLRQAFSFHTLLVGGNPFTTSSIYTLIHSLEQRWGV
                     HFPKGGTGALVQGLVRLFGDLGGELKLNTPVAEILTSGDRVTGVRTDNGWEGRFDAVA
                     SNADVVFTYERLLGRTARGRAAARALRRKRFSPSLFVVYFGVRGSYPELKHHSILFGP
                     RYRELLKEIYAGPGLPDDFSLYLHAPSVTDPSLAPPGCSTFYVLSPVPNLKAADLDWS
                     ELGPRYRDHILAYLEKHYLPGLGERLVTTRTFTPDDFQTQLNAHLGSAFSLEPVLWQS
                     AWFRTHNRDDVLKGLYFAGAGTHPGAGIPGVVGSAKATSRLMLDDFGRTYPDSDRSDQ
                     FAKEMA"
BASE COUNT          541 a          958 c          940 g          517 t
ORIGIN      
        1 gatcgcgccg gaagtgcgaa cgagcatcgt cggccgcctg gcggacgcga tcggaatcca
       61 agggctctct gccgggcagg agcgcgatct gcatgaggcc cagagtacga ccagccgggc
      121 cgctctcgag aagcgccatc atcagaaaac gggcgctctg ttcgtggcgt gcctggaagc
      181 cggcgcccgc atcgccggcg tggacgagac caccatggcc gcgatgacga aattcggcga
      241 gcgggtcggc atcggtttcc agattctgga cgatcttctg gacctgtccg catccgagtc
      301 caccggtaag gacgctcatc gcgacgaggg caaagccacc ttcgccaccg tgatgacgat
      361 tcaggaagcc gaacgtgaag ccatcacctg cctcgataac gcgcttgccg aactggccga
      421 ccagatcgca gacgcggggc cgttcgccgt tttcgtggat ctggtggttc gcgcctacgc
      481 cgatcaggtg agcaggaggg ggtccgagat cgctggcgcg ttgcggccgt gaccgtcgtc
      541 gtcaacgccg cctgtttcct gaccgctctc gccatcatgg aatgggtggc ctgggtcacc
      601 cacaagtacg tgatgcacgg gtttctctgg agcctgcacg aatcgcatca tcagccccga
      661 aaggggcgct tcgagcgcaa cgactggttt gccgtgttct ttgccgtacc gtccatcgtg
      721 ctcatctatc tcggcaacaa tggctatccg gccgcgctct ggggcgggct cggcgtcgcg
      781 gcctacgggc tgatctactt catctttcac gacgtgatcg tccaccgacg cctcgacacc
      841 ggttacgcgc cgcgcagccg ctacatgcgt cgtatcgttc acgcgcatcg ccttcaccac
      901 gccgtgtccg aaaagcatgg cgcggtatct ttcggcttcg tctacgcgcc gccgattccc
      961 gtattgcggg cggagatgaa agccctggcg gcctccgggg cgtcaggaag ctgaccagag
     1021 cccacgtctg ataggggccg tccggcgtcg cccgaacgta acctgcagtc ccgctaacgc
     1081 tgctccttta agaagcagca cgagctttcg gcctacgccg acggtcgccc ggtgctgcca
     1141 gacgtcaggc ccgtcacggc gaacgacccg accaatgtcg cgatagatgg caagcgcgct
     1201 ggcgatcgcc caggcattgc gaaacctaag gcggccaatg ccgtgtcttg ccgactcgta
     1261 gtagcgctcg gcctcgtcga gaatccgaat ggtctgaccg tagatgacgt cgcggtggtc
     1321 gggaaactgc ccggtgtcct cgggcacccc ggcgtcggcg agccattgcc tgggcaggta
     1381 gcagcgtccg tcggcagcgt cctgcatgac gtcgcgggca atgttggtca gttgaaacgc
     1441 gatccccagg tcgacggccc gagccaccac ctctggatca cgaatcccca tgaccgtcgc
     1501 catcatcccg cccacggtcc ctgccacgtg atagcagtaa cgcagcgtgt cgctgagcgt
     1561 gcggcactca tgaccctcga cgtccatggc gaacccatcg agcaattcca gcgggtagtt
     1621 ccggggaatg tcgcattcga gaaagactct ctgaaaggcg acgaacgggg ttccgtcgac
     1681 cgcctcaccg cgcatggccg cttcggtatc ccgccgcaac tgctccagcc gcgcgcgctg
     1741 ctccggaggt gccggagagc gctgcccgat gccgagatcc tggccgtcga tctcgtcatc
     1801 gcagtggcgg caccacgcat atagcatgat cgcgtgaccc cgggtcgtcg ggtcaaacag
     1861 gcgcgcggcc gcagcgaagc ttttcgagcc cctggagatg atcgacgcgc tggctgcgaa
     1921 aacgtcactc actcaggcca tctccttggc gaactgatcc gaccggtcgc tgtccggata
     1981 cgttcggccg aagtcatcga gcatcaggcg cgatgtggcc ttggcggagc cgaccacgcc
     2041 gggtatcccc gcgcccgggt gcgtgcccgc tccggcaaag taaaggcctt tgagcacgtc
     2101 gtcgcggttg tgcgtgcgga accaggcgct ttgccatagc acgggttcta aagaaaacgc
     2161 cgagccaaga tgcgcgttga gctgcgtctg aaagtcatcc ggcgtgaacg tgcgggtggt
     2221 gaccagacgc tcaccgagcc ccgggagata gtgcttttcc aggtacgcaa gaatgtgatc
     2281 ccggtaacgc gggccaagct cggaccagtc gaggtcggcc gccttgagat tgggtaccgg
     2341 cgacaacacg tagaaggtcg agcagcctgg cggggcgaga gaggggtcgg taacgctggg
     2401 cgcgtgcaga tacaacgaga aatcgtccgg cagcccggga ccggcgtaaa tttccttgag
     2461 aagctcccgg taacgcggac cgaagagaat gctatggtgc ttcagttcgg ggtagctgcc
     2521 cctgacgcca aagtaaacca cgaacagcga ggggctgaat cttttgcgcc gcagcgcgcg
     2581 ggcggccgct ctgccccgcg ccgtccggcc aagcagcctt tcataggtga acaccacgtc
     2641 ggcgttgctg gccacggcgt cgaaccgtcc ctcccagccg ttgtccgtcc ggacacccgt
     2701 gacgcggtcg ccgctggtca atatctccgc caccggcgtg ttgagcttga gctcgcctcc
     2761 aagatcgccg aaaaggcgga ccagcccctg caccagagcg ccggttccac cctttggaaa
     2821 gtgcacgccc cagcgctgct cgagggagtg aatcagcgtg tagatggacg aggtcgtgaa
     2881 cgggttgccc cccaccagaa gggtgtggaa gctgaacgcc tgacgcaggt gctcgtcctc
     2941 gatgaatttc gacacc
//
LOCUS       DBNHQR010000593         3584 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1578244, whole genome shotgun sequence.
ACCESSION   DBNHQR010000593 DBNHQR010000000
VERSION     DBNHQR010000593.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1578244"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..264)
                     /locus_tag="AC16VR_13520"
     tRNA            complement(188..264)
                     /locus_tag="AC16VR_13520"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(228..230),aa:Arg,seq:cct)
     gene            complement(339..1472)
                     /locus_tag="AC16VR_13525"
     CDS             complement(339..1472)
                     /locus_tag="AC16VR_13525"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014596192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HIR6012224.1"
                     /translation="MDLLSQIWEGSGLNQLTWGQAIMIGVGLVLLYLAIARQFEPLLL
                     VTIGFGGILANIPGAGIAEGDGLLHLAYVVGIESGAFPLIIFMGVGALTDFGPLLANP
                     KTLFLGAAAQFGIFATLLGALGLSELGVFDFGLREAAAIGIIGGADGPTAIYVAGRLA
                     PELLGAIAVAAYSYMALVPLIQPPIMRALTTEAERKIEMVQLREVSRLERIVFPLLLL
                     LLVALLLPSAAPLLGMFCFGNLMRECGVVDRLSDTTQNALINIVTIFLGLAVGSKLSA
                     EQFLVPSTLGILALGMAAFALGTASGVLMGKLLNLVTREHINPLIGAAGVSAVPMAAR
                     VANRVGLEANRHNYLLMHAMGPNVAGVIGSAVAAGIMIMLVGD"
     gene            complement(1475..3262)
                     /gene="oadA"
                     /locus_tag="AC16VR_13530"
     CDS             complement(1475..3262)
                     /gene="oadA"
                     /locus_tag="AC16VR_13530"
                     /EC_number="7.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-extruding oxaloacetate decarboxylase
                     subunit alpha"
                     /protein_id="HIR6012225.1"
                     /translation="MSTKRPLGITDVVLRDAHQSLLATRMRVEDMLPIAGKLDQVGFW
                     SIESWGGATFDACIRYLGEDPWERMRKLKAAMPNTPQQMLFRGQNILGYRHYADDVVG
                     KFVERAAANGVDVFRIFDALNDIRNLRTAIRATLDVGRHAQGTISYTVSPVHTMEVWV
                     DLGKQIEDTGAHSIAIKDMAGLLKPYVAFELVSRLKESVSVPIHMQCHATTGLSTATY
                     LKAIEAGIDNVDTAISSMSMTYGHSPTETLVSILEGGDRDTGLDISLLEEIAAYFRQV
                     RKKYAKFEGTLKGVDSRILVAQVPGGMLTNLESQLREQNASDRLDEVLDEIPRVREDL
                     GFIPLVTPTSQIVGTQAVINVLMNERYKNVTKETAGVLKGEYGATPAPVNADLQARVL
                     NGESPITGRPADHLQPEVEALTGELQELAAERGFALAGDVVDDVLTYALFPQVGLKFL
                     ENRGNPDAFEPAPWVVEVPALEAGRVVPAPPAPPRASTYNVRVNGRQFTVEVSEAGAV
                     SSVKPAEPAFKAPVRAGAGTIIAALAGNVFKVLVQPGDVVTEGQTILVVEAMKMETAV
                     AASQDGTVAEVHVREGDAVAVGDPLVTLA"
     gene            complement(3273..3467)
                     /locus_tag="AC16VR_13535"
     CDS             complement(3273..3467)
                     /locus_tag="AC16VR_13535"
                     /inference="COORDINATES: protein motif:HMM:NF016187.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HIR6012226.1"
                     /translation="MVQGLHLMLAGMATVFVFLSVLVGATVVMSRVLMRLQGEPGPTA
                     DEVAAIVAAIERHRASGPDR"
BASE COUNT          683 a         1203 c         1102 g          596 t
ORIGIN      
        1 cagagagcca ctggcggtgg ctggcgccag cgcgaggact accagagctc gtacgccagc
       61 tgcacgggat ccaacgtcta ccggaccaca attctccaca gaccgtaacc ccaaccctaa
      121 cgtcacgtcg ccacgctccg tggcgacgtg acgatggccg cggcccgttc gggaaagcac
      181 gaattactgg cgccccgaga aggattcgaa ccttcgacct gccccttagg agggggccgc
      241 gctatccagc tgtgccatcg gggcggatgc tccgctcggc gaagcccagc aaagataccg
      301 caccagctgc tgcgaacgct acgggctcga ctaggcgttc agtcacccac cagcatgatc
      361 atgattccag cggccacggc cgatccgatc acgcccgcca cgttcgggcc catcgcgtgc
      421 atgagcagat aattgtggcg attggcttcg agcccgaccc ggttggcgac ccgcgccgcc
      481 atggggaccg ccgagacgcc ggcagcgccg atcagcgggt tgatgtgctc gcgggtcacc
      541 aggttcagca gcttgcccat cagaacgccg gacgcggttc ccagcgcaaa cgcggccata
      601 ccgagtgcca ggatgcccag cgtgctgggc accaggaact gctctgcgct tagcttggag
      661 cccacggcaa ggccgaggaa gatggtcacg atgttgatca gggcgttctg ggtggtatcc
      721 gacaaccgat ccaccacgcc gcattcgcgc atcaggttac cgaagcagaa catccccagc
      781 agaggcgcgg cagatggcaa cagcagcgcc accagcagca gcagcagcag cggaaaaacg
      841 atgcgctcga gcctggagac ctcgcgcagc tgtaccattt cgatcttgcg ctccgcctcg
      901 gtggtcaggg cgcgcatgat gggcggctgg atcagcggca ccagggccat gtaggagtaa
      961 gcggccacgg cgattgctcc gagcagctcc ggggcgagcc tgcccgcgac gtatatcgcc
     1021 gtaggcccgt cggcgccgcc gatgatcccg atggcggcgg cctcgcgcag cccgaagtcg
     1081 aagacgccga gctccgagag gcccaacgcg cccaggaggg tggcaaaaat gccgaactgg
     1141 gccgcggcgc cgagaaagag cgttttcggg ttggccagga gcgggccgaa gtcggtgagc
     1201 gccccgacgc ccatgaagat gatcagcgga aaagcgcccg actcgatccc caccacgtag
     1261 gccaggtgca gcagaccgtc gccttcggcg attcccgcgc cgggaatgtt ggccagaatc
     1321 ccaccgaaac cgatggtcac gagcagcaga ggctcgaact gacgggcaat ggccaggtaa
     1381 agcagcacga ggcccacgcc gatcatgatg gcctgacccc aggtgagctg gtttaagccc
     1441 gaaccctccc agatctgact cagcaggtcc acgatcaggc cagcgtcacc aacggatcac
     1501 ccaccgcaac cgcatctccc tcgcggacgt gaacttcagc cacggtgccg tcctgagagg
     1561 ccgctacggc cgtctccatc ttcatggcct ccaccaccag gatcgtctgc ccctcggtga
     1621 ccacgtcccc cggctgcacg agcaccttga agacgttgcc tgcaagcgct gcgatgatcg
     1681 taccggcacc tgcccgaacg ggcgccttga aggccggttc agccggcttg acgctggaca
     1741 cggcgcccgc ttcggaaacc tccacggtga attgcctgcc gttcaccctc acgttgtagg
     1801 tgcttgcccg agggggtgcg ggcggcgccg gcaccaccct gccggcttcc aatgcaggca
     1861 cttccaccac ccagggcgca ggctcgaacg catcggggtt gcctcgattc tcgagaaact
     1921 tgagccccac ctggggaaac aaagcgtaag tcaggacatc gtccacgacg tcgcctgcca
     1981 gcgcgaaacc ccgttccgcc gcaagttcct gcagttcgcc cgtcaacgct tccacctctg
     2041 gctgcagatg atctgccgga cgaccagtga tcggcgactc cccgttgagc acacgggcct
     2101 gaagatccgc gttgaccggg gccggcgttg cgccgtattc ccccttgagc acgccggccg
     2161 tctccttggt gacgttcttg tagcgctcgt tcatcaggac gttgatgacc gcctgggtgc
     2221 caacgatctg ggaggtgggc gtaaccagag ggatgaaacc gagatcctca cggacacggg
     2281 ggatctcatc gagcacctca tcgagccggt ccgaggcgtt ctgctcgcgc agctggctct
     2341 ccaggttcgt cagcatcccg ccaggcacct gggctaccag aatgcgcgag tccacgccct
     2401 tgagcgtgcc ctcgaatttt gcgtacttct ttcggacttg gcggaagtag gcggcaatct
     2461 cctccagcag cgagatgtcg agcccggtgt cgcgatcgcc gccctcgagt atcgacacca
     2521 gcgtctccgt aggcgagtga ccataggtca tgctcatgga cgagatggcc gtgtccacgt
     2581 tgtcgatgcc ggcctcgatg gccttgaggt acgtggccgt cgacagcccc gtcgtggcat
     2641 ggcactgcat gtggatgggc acgctgaccg actccttcag gcggctcacc agctcgaagg
     2701 ccacataggg cttgagaagc ccggccatgt ccttgatggc gatggagtgc gcgcccgtat
     2761 cttcgatctg cttgcccagg tcgacccaga cttccatggt gtgcacgggg ctgacggtgt
     2821 acgagatcgt cccctgggcg tgcctgccca catcgagcgt cgcgcggatg gcggttcgca
     2881 gattcctgat gtcgttgagc gcatcgaata tccgaaaaac gtcgaccccg ttggccgccg
     2941 cgcgctcgac gaatttgccc acgacgtcgt ccgcataatg ccggtaaccc agaatgttct
     3001 ggccgcggaa gagcatctgc tgcggtgtgt tcggcatggc cgctttaagc ttgcgcatcc
     3061 gctcccacgg atcttcaccc agataccgga tgcaggcatc gaaggtcgcg ccgccccagg
     3121 attcgatgga ccagaacccg acctgatcga gcttgccggc aatcggcagc atgtcctcga
     3181 cgcgcatgcg ggtagccagc aggctctgat gggcgtcacg caggaccacg tcggtgatcc
     3241 ccaacggtct cttggtgctc atgggtacct catcatcggt ccgggccgga ggcgcgatgc
     3301 ctttcaatgg ccgcaacgat ggctgccact tcgtccgcgg tggggccggg ctcgccctgc
     3361 agccgcatga gcaccctgga catgacgacc gtcgcgccga cgagaaccga gagaaagaca
     3421 aacaccgtcg ccatgccagc cagcatcagg tgcaaaccct gaaccatcag atcagacatc
     3481 tccggaccga ttgcgccact aaaactcggg ttgcgcattc tgcatcaggt gcccgggcaa
     3541 aggtagccgc agcgccgcgc tcctatgaac tgtcggccct ttct
//
LOCUS       DBNHQR010000594         1410 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1135163, whole genome shotgun sequence.
ACCESSION   DBNHQR010000594 DBNHQR010000000
VERSION     DBNHQR010000594.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1410)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1135163"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="AC16VR_13540"
     CDS             complement(<1..462)
                     /locus_tag="AC16VR_13540"
                     /inference="COORDINATES: protein motif:HMM:NF012363.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HIR6012227.1"
                     /translation="MSQPNIAQTRTGAVEGRMKDGTLLFSGIPYAAAPEGSLRFRPAA
                     PVTPWTGVRPALKFGPAAPQVATGGLTNSAAVRWSEDCLSLNVCTPALDGALRPVLVW
                     IHGGSFRTGQGAVPWYNGNRFALHGDMVVVSINYRLGALGFSELSRLGSGFE"
     gene            complement(472..1371)
                     /locus_tag="AC16VR_13545"
     CDS             complement(472..1371)
                     /locus_tag="AC16VR_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019691519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HIR6012228.1"
                     /translation="MTRYQIVHKTHYEYARPVSSSYQHLHLTPRSFAKQNVISSDLLI
                     EPAPTSNQTRQDYFGNPVTDVVIRSRHDSLEITSRALLDVNADENILLDLSPPWEAVA
                     GAVGDPRSSEAWDAARFCFPSPHVDPREALAYAEPSLSPGTPFLRSVEHITARIYEEF
                     TYKGGVTDVQTPVEEVLQSRAGVCQDFAHLAIASVRAFGLPARYVSGYLLTRPPQGQP
                     RLTGADASHAWISVWCPEFGWIDFDPTNNLRPRDEHITLGWGRDYSDVSPTRGFIHGG
                     GSQQLEVSVDVTPVPLADHRARR"
BASE COUNT          248 a          435 c          481 g          246 t
ORIGIN      
        1 ctcgaaaccc gatccgaggc gggacagctc ggagaatccc agcgcgccca gccggtagtt
       61 gatgctgacg acgaccatgt cgccgtgaag cgcgaaccgg ttgccgttgt accagggcac
      121 cgctccctgg ccggtgcgga agctgccgcc gtgaatccac acgagcaccg ggcgcagcgc
      181 tccgtccagc gccggggtgc agacgttgag ggataggcaa tcctcggacc agcgaacagc
      241 ggcgctgttg gtgagaccgc cggtcgccac ctgaggagcg gcgggtccga acttcaacgc
      301 cgggcggact ccggtccagg gcgttaccgg tgccgcaggc ctgaaacgaa gggaaccctc
      361 cggtgcagcg gcgtagggta ttccggaaaa caggagcgtg ccgtctttca tgcggccttc
      421 tactgcaccg gttcgggtct gggcaatgtt tggttggctc atgggaagag gttaccggcg
      481 ggcgcggtga tcggccagcg ggacgggtgt cacgtccacg gaaacttcca gctgctggct
      541 tccaccgccg tgaatgaatc cccgggtggg gctgacgtcg ctgtagtcgc ggccccagcc
      601 cagcgtgatg tgctcgtccc ttgggcggag attgttggtc ggatcgaagt cgatccagcc
      661 gaactccggg caccacaccg atatccaggc gtgggacgca tccgcgccgg tgagccgagg
      721 ctgtccttgg ggaggtcggg taagcaggta gccgctgacg tacctggccg gcagcccgaa
      781 cgcccgcacg ctggcgatgg ccaggtgagc aaagtcctgg cacaccccgg ccctcgactg
      841 cagcacctct tccaccggcg tctggacgtc ggtgacgccg cccttgtagg tgaactcctc
      901 gtagatccga gccgtgatgt gctcgacgga tcgcagaaat ggcgtgccgg gcgacaggct
      961 gggctcggcg taggcaagag cttcccgcgg atcgacatga ggcgagggaa agcagaagcg
     1021 ggcggcatcc caggcctcgc tcgatctggg atcgccgacc gccccggcga cggcttccca
     1081 cggcgggctc agatccagaa ggatgttctc atctgcattc acgtcaagca gcgcacgact
     1141 ggtgatctcg agcgagtcgt gtctgctgcg gatgacgacg tcggtgaccg ggttgccgaa
     1201 gtaatcctgc cgcgtctggt tcgaagtggg ggccggctct atgagcaggt ccgagctgat
     1261 cacgttctgc ttggcaaagc tgcgcggcgt gaggtgcaga tgctggtagg acgagctcac
     1321 cggacgagcg tactcgtagt gcgtcttgtg aactatctga taacgcgtca tctcacaccg
     1381 cctttttcag ccacctcaat aaccctccgt
//
LOCUS       DBNHQR010000595         4189 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_312404, whole genome shotgun sequence.
ACCESSION   DBNHQR010000595 DBNHQR010000000
VERSION     DBNHQR010000595.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_312404"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="AC16VR_13550"
     CDS             <1..504
                     /locus_tag="AC16VR_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HIR6012229.1"
                     /translation="SNIQSDTVSELDVASGKQVRAYPTPKQPEAIAVTADGSEIWVGS
                     NAEGSLSVIRTDDATIETIAEGFGWPYRILISRDGRLVLVPDLKRNVLRMFDRTNRVE
                     LASVELADAAPQGLVFHPEERWLFVSLNGQNQVAVIDLSRSAIVGRIDTGPRPDGIGY
                     AATKGQP"
     gene            501..1139
                     /locus_tag="AC16VR_13555"
     CDS             501..1139
                     /locus_tag="AC16VR_13555"
                     /inference="COORDINATES: protein motif:HMM:NF013695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation efflux transmembrane domain-containing
                     protein"
                     /protein_id="HIR6012230.1"
                     /translation="MSDDCCANGIDASGLETRHRRALASVLAINVAAFLVMIVGAVLS
                     GSSSLLSGTLDNLGDALTYALSLAVIGSTASAKARVAFFKGLLIFAAALGVAAQIGWR
                     LSNMETPVFETMGVAATLNLAANLICLRILFPFRNDDLNMTSVWECSRNDIFDGLAVL
                     TATVAVWVSDSAWPDLLIAIALLIVFLRSAARILSRSWRELHAAPTPGPGTG"
     gene            complement(1173..2276)
                     /locus_tag="AC16VR_13560"
     CDS             complement(1173..2276)
                     /locus_tag="AC16VR_13560"
                     /inference="COORDINATES: protein motif:HMM:NF014954.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR6012231.1"
                     /translation="MAGIPETPEDFTTSWLCEVLGVPESEVLDVDVRTSEADSGFVSL
                     ACFLELETRPGSGLPRQLVGKLAPTFEGARKLSREFGLFAREKQFYSEIAPLVAIRSP
                     GAYHCDYDAELGTGVLLLEDCSHMRAPVQTDEQPTTLAELELIVDAAADMAISSWNAD
                     WLATKDELMGPDHPAFVGYFSGIQEGYKDFVDSEFMSWAPDGFEGLAVRLGENMRTIA
                     AAQPETNLSLCHMDLRLANVFLDEESDDPVVLYDWQSMYPGRTAQDLGYLITSGYTPA
                     FRREHERNLVSRYHQRLVDAGIPGYSFDEAWFDYRHGIIIGLRLLPMALGDLDVSTEG
                     GQAIFRKIIRNFPEAALDHGGVELVDEILARRA"
     gene            2426..3205
                     /locus_tag="AC16VR_13565"
     CDS             2426..3205
                     /locus_tag="AC16VR_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012232.1"
                     /translation="MKTINRTLGRSLLVLIPFLALNGMFAYADSHGPALPSGVLEVFQ
                     CNYNSGKDRDDLNALRDFYLKQAKKVNYTPPDAFLWTMTNGSWPTTLTWVNVHEDLSA
                     YSRSYDTIAASTDLAAVNERAETVMTCVPALGSYAHAFIRDPDAQGPWEATVASYACD
                     LRPEQDMSDLEDLMEHVATVNQSMGDDAMDELLHFIPMTGLNNQPDVLVFAASRRGAS
                     SWSSHLALLEQSGAGRAVLAHFDSVLDCRLSLWDVEQMVGG"
     gene            complement(3226..>4189)
                     /locus_tag="AC16VR_13570"
     CDS             complement(3226..>4189)
                     /locus_tag="AC16VR_13570"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
                     /protein_id="HIR6012233.1"
                     /translation="FARGYKPGGLNGFFSTNPTYDREDVNSFELGFKSLLADDSLMLN
                     VAAFFNDYEGLQLTNLAAEQFTRNIENTNVDADMYGAEIEARWRPPFAPRAELEIGYS
                     WLNAELKNEPPRIDALWLTNGDPEYVELLSFATNAGSPNGRYVARVEDVLPWVNPAIA
                     IGAAIGPDEAPATIYPNGIPAWFDGAFLSAVGVELLPGIPVPVSGNSVPDTPEHTVHL
                     GVSYTWDVPAGALTARWDYYWQSKSYLTIFNRSAETIDAWDQHNLSLIYETGDGRWSA
                     RAWVRNIENDLHILGGYRQHSQQDFSVSEPRAFGVSLRFNFAPG"
BASE COUNT          782 a         1353 c         1276 g          778 t
ORIGIN      
        1 agcaacatcc agagcgacac ggtctccgag ctcgacgtgg cctccgggaa acaggtgcgc
       61 gcctacccaa ccccgaaaca acccgaagcc atcgcagtga ccgctgacgg ctcggaaatc
      121 tgggtcggca gcaacgctga aggctcgctg agcgtgatcc gaaccgatga cgccaccatc
      181 gagacgattg ccgaaggctt cggttggccg taccgtatcc tgatctccag ggacggcagg
      241 ctggtgctgg tcccggacct caagaggaac gtgctgcgca tgttcgatcg caccaatcgg
      301 gtagagctag ccagcgtgga gttggcggac gcggcgcccc agggactcgt cttccatccg
      361 gaagaacgct ggcttttcgt ctcgctcaat ggtcagaatc aggtggccgt catcgatctg
      421 agcagatccg ccatcgtcgg gcgcattgac acgggcccac ggcctgacgg cattggctac
      481 gccgcgacga aggggcagcc atgagcgacg attgctgcgc caacggaatc gacgcgagcg
      541 gtctcgaaac gcggcaccgg cgcgcgctgg cgtccgttct cgccataaac gtcgccgcat
      601 tcctcgtgat gatcgtgggc gccgtgctca gcggctcatc ctctctgctc tccggcaccc
      661 tggacaacct cggcgacgcg ttgacctacg cccttagcct ggcagtcatc ggttccacgg
      721 catccgcaaa ggcccgggtc gcctttttca agggcttgct gatctttgca gccgccctcg
      781 gggtggccgc gcagatcggc tggcggcttt ccaacatgga gacgccggtg ttcgaaacca
      841 tgggggtggc ggccaccctc aacctggccg cgaacctgat ctgcctgcgc atcctgtttc
      901 ccttccggaa cgacgacctg aacatgacgt ccgtctggga gtgctcgcgc aacgacatct
      961 tcgacggcct tgcagtgctg accgccaccg tggccgtgtg ggtttccgac tccgcatggc
     1021 cggacctctt gatcgcgatc gctctgctga tcgttttcct gcgatccgct gcccgcattc
     1081 tcagccgttc ctggcgggag ctgcacgccg cacccacgcc ggggccgggg accgggtagg
     1141 aaaacggcga ccggatgcag cgtcaggcag atctatgctc gccgggccag aatctcatcc
     1201 accagctcga cgccgccgtg gtccagggct gcctcgggga agttccgaat gatcttcctg
     1261 aagatggcct ggccgccctc ggtggagacg tccagatcgc ctaacgccat cggcaacagc
     1321 cgcaggccga tgatgatgcc gtgccggtag tcgaaccagg cttcatcgaa gctgtaaccc
     1381 gggatccccg cgtccaccag ccgctggtgg taacgactaa ccaggttacg ttcatgctcg
     1441 cgacgaaacg ccggcgtgta ccccgaggtg atcagatacc ccagatcctg ggccgttctg
     1501 ccgggataca ttgactgcca atcgtagaga acgaccggat cgtcggattc ctcatcgagg
     1561 aacacgttag ccagccgtaa atccatgtgg cagagagaca gattggtttc cggttgcgca
     1621 gcagcgatcg tccgcatgtt ctcgccgagc cgaacggcca gaccctcgaa cccgtcgggc
     1681 gcccaggaca tgaactcgct gtcgacgaag tccttgtagc cctcctggat ccccgagaaa
     1741 tagcctacaa aggcggggtg atcgggcccc atcagctcat cctttgtcgc gagccaatcc
     1801 gcattccagg aagaaatcgc catgtcagcg gcagcgtcga cgatcagctc cagctctgcc
     1861 aaggtagtcg gctgctcgtc ggtctgcacc ggcgcgcgca tgtgcgagca atcctcgagg
     1921 agcagaacac cggttcccag ctcggcatcg taatcgcagt gataggctcc cggtgagcgg
     1981 atggctacga gcggagcgat ttccgagtaa aactgctttt cgcgcgcgaa gagcccgaac
     2041 tcgcggctta gtttcctcgc tccctcgaat gtcggcgcca gcttgcctac gagctggcgg
     2101 ggcaacccgc tcccgggccg tgtctccagc tccagaaagc acgcgaggct cacgaagccc
     2161 gagtccgctt cggaggtcct cacatcgaca tcgaggactt cgctctcagg cacgccaagc
     2221 acctcgcaca gccagctcgt ggtgaagtcc tctggagttt caggtatgcc tgccatctcg
     2281 ctggttctcc tctctatccc gatttctcat cccaccgacg ccacgacctt gccatggcgt
     2341 cgggaattag gaaagtcgcc ccggcgcagc cacttgccgt aacattcgtg ccggaccgcg
     2401 atgaccagag tttaaggaga caacgatgaa aacaatcaac cgaacgttgg gtcgatccct
     2461 gctggtgctg attcccttcc ttgccctgaa cggcatgttc gcctacgccg attcccacgg
     2521 gccggctctc ccatcgggcg tgctcgaagt ttttcagtgc aactacaaca gcggcaagga
     2581 tcgggacgac ctgaacgccc tgcgggactt ctacctgaag caggcgaaaa aggtgaacta
     2641 cacgccgcct gacgcgtttc tctggacgat gaccaatggc agctggccca ccacccttac
     2701 gtgggtcaac gtccacgaag acctgagcgc ctattccaga tcttacgata ccattgcggc
     2761 ctcgaccgac ctggccgcgg tcaacgagcg cgccgagacg gtaatgacct gcgtgcccgc
     2821 ccttggaagc tacgcccacg cgttcatacg cgaccccgac gcccaaggac cctgggaggc
     2881 gacggtggct tcctacgcct gcgatctgcg ccccgagcag gacatgagcg acctggaaga
     2941 tctaatggaa cacgtggcaa cggtcaatca atccatgggc gacgatgcca tggatgaact
     3001 gctgcacttc attcccatga cgggtctaaa caaccagccg gatgtgttgg tattcgcggc
     3061 gagccggcgc ggcgcttcgt cctggtcgag ccatctcgcg ctcctcgagc aatcgggtgc
     3121 cgggcgggca gttctcgcgc actttgattc cgtgctcgat tgccggttga gcctttggga
     3181 cgtggaacag atggtggggg gataaccgcg ggcacgccgc ccggctcagc ccggggcgaa
     3241 attgaatcgc aacgacacgc cgaacgcccg cggctcgctc acggaaaaat cctgctgcga
     3301 atgctgacgg tacccaccta ggatgtgcaa gtcgttttcg atgttgcgca cccaggcgcg
     3361 cgccgaccag cgtccgtcgc cggtctcgta gatcaggctc aaattgtgct gatcccaggc
     3421 gtcgatggtc tccgcgctgc gattgaaaat cgtcaggtag ctcttgctct gccagtagta
     3481 gtcccaccgg gcagtgagcg ccccggcggg cacgtcccac gtgtagctga cacccaggtg
     3541 caccgtgtgc tcgggcgtgt cggggacgga gttccccgac accggcacgg gtatcccggg
     3601 taacagctcc acgcccacgg cagagagaaa tgccccgtcg aaccaggccg ggatgccgtt
     3661 gggatagatg gttgcgggcg cttcatccgg cccgatggcc gctcctatgg caatcgcggg
     3721 gttcacccag ggcagcacgt cctcaacgcg ggcaacgtag cggccattgg gagatccggc
     3781 gttggtggcg aaagacagca gctccacata ttcggggtcg ccgttggtca gccacagggc
     3841 gtcgatgcgg ggtggttcat tcttgagctc ggcattgagc caggagtagc caatctccag
     3901 ctcggctctc ggcgcgaagg gcggtcgcca cctggcctcg atctccgcgc cgtacatgtc
     3961 cgcgtccacg ttggtgttct cgatgttgcg ggtaaactgc tccgcggcca ggttggtgag
     4021 ctgcagcccc tcatagtcgt tgaagaatgc ggccacgttc agcattaggc tgtcgtctgc
     4081 cagcagcgac ttgaagccga gctcgaaaga gttgacgtcc tcgcggtcgt aggtcgggtt
     4141 ggtgctgaag aagccgttca ggccgccggg cttgtagccc cgggcgaag
//
LOCUS       DBNHQR010000596         2654 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_59679, whole genome shotgun sequence.
ACCESSION   DBNHQR010000596 DBNHQR010000000
VERSION     DBNHQR010000596.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_59679"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="AC16VR_13575"
     CDS             complement(<1..894)
                     /locus_tag="AC16VR_13575"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003860 - 3-hydroxyisobutyryl-CoA
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6012234.1"
                     /translation="MEPVNSANAPVQLFEHPTGSGHVLAEARLNFEGTLNSLSLEMID
                     IMSPALADWSGRAEIVAVLFTGAGDRAFCAGGDIQALYHAMVKNHEHGEVVDDYPFQF
                     FEREYRFDHQIHTFPKPVIAVGHGVVMGGGLGILSAARYRLVTERSRIAMPEVTIGLF
                     PDAGQTWLLRNMPEHFATFLGVTGSHMNGADALMVGIGTHGIEQASRESVRERLCAAS
                     WQADAHAAVQEALADLPAGGFPEAQVERLPDSLSVAGGIADVAASIAALRGGSDWIDR
                     GIGAMQSGCPTTVGIVVEQLRR"
     gene            complement(902..1690)
                     /locus_tag="AC16VR_13580"
     CDS             complement(902..1690)
                     /locus_tag="AC16VR_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008175944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HIR6012235.1"
                     /translation="MIESPTEKLLVEQRGHTLLVTINTPPANTWDAESLQGLKNVVEQ
                     ANAERSVYAIVITGQGEKFFSAGADLKLFADGDPAVASEMAGQFGSAFETLSAFRGVS
                     IAAVNGFAMGGGLECALACDIRVAEAQAKLALPEATVGLLPCGGGTQNLAWMVGEGWA
                     KRMILCGERVDAATALKIGLVEEVVDAGQSLGRALELAEMVGRQSPPAVAACKDLIQQ
                     ARSGNISKAYSTERAAFVGLFDTQDQKEGVNAFLEKRSPEWVNG"
     gene            complement(1746..2462)
                     /gene="ung"
                     /locus_tag="AC16VR_13585"
     CDS             complement(1746..2462)
                     /gene="ung"
                     /locus_tag="AC16VR_13585"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR6012236.1"
                     /translation="MVEAARRSEERVRLEPSWKAVLGGEFDKPYMNKLREFLLREKRA
                     GKVIYPRGDNIFAALDLTSSKGVKVVIIGQDPYHGPDQAHGLCFSVQPGIPQPPSLVN
                     IFQEINEELGGPAKRLDDTNGCLEPWARQGVLLLNAVLTVERGRAGSHQGMGWEIFTD
                     KVVDVLNRERDGLVFLLWGSYAQKKGAILDVQRHCVLKAPHPSPLSAHRGFFGCGHFA
                     RANEYLQQRGVAGIDWFDVA"
     gene            complement(2455..>2654)
                     /locus_tag="AC16VR_13590"
     CDS             complement(2455..>2654)
                     /locus_tag="AC16VR_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019366559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HIR6012237.1"
                     /translation="QALLRSGGDVEAMHFDDDYITALEYGLPPTAGEGLGIDRLVMLL
                     TDSPTIRDVLLFPLMRPVQDG"
BASE COUNT          477 a          927 c          794 g          456 t
ORIGIN      
        1 tcggcgcagc tgctccacga cgatccccac cgtggtcggg caacccgact gcatggcgcc
       61 gataccgcgg tcgatccagt cgctgccgcc acgcagggcg gcaatggacg cggccacgtc
      121 agcgatgcca cctgccacgg acagcgagtc cggcaggcgt tcgacctgcg cttcgggaaa
      181 cccgcccgcc ggcaggtcag ccagggcttc ctgcaccgcg gcatgcgcgt ctgcctgcca
      241 ggaggccgcg caaagccgct cgcgcacgct ttccctggac gcctgctcga tcccgtgagt
      301 gccgataccc accatcaggg cgtccgcgcc gttcatgtga gagccggtca ctccgagaaa
      361 cgtggcgaag tgctcgggca tgtttctcag cagccaggtc tgcccggcgt ctggaaacag
      421 accgatggtc acctcgggca tggcaatccg cgaccgctcc gtcaccagac ggtaccgcgc
      481 ggcagagagg ataccgagcc cgcctcccat gacgaccccg tggcccacgg cgatcaccgg
      541 cttgggaaaa gtgtggatct gatggtcgaa gcggtattcc cgctcgaaga actgaaacgg
      601 gtaatcgtcc accacctcac catgctcatg gtttttcacc atggcgtggt agagcgcctg
      661 aatgtccccg ccggcgcaga aagcgcgatc gccggccccg gtgaacagaa cggcgacgat
      721 ctcagcacgg ccggaccaat ccgcgagcgc cggggacatg atgtcgatca tctccaggct
      781 gagcgagttg agggtgccct cgaaattgag cctcgcttcc gcaagaacgt ggccgctccc
      841 cgtaggatgc tcaaagagct gcaccggcgc gttggctgaa ttgactggtt ccatgacgcc
      901 gttacccgtt gacccattca ggcgaacgct tctcgaggaa agcgttgacc ccttctttct
      961 ggtcctgcgt atcgaacaga cccacgaagg cggcccgctc cgtcgagtag gctttgctga
     1021 tgttgccgct gcgcgcctgc tgaatgagat ccttgcaggc ggccaccgcg ggcgggctct
     1081 gccggcccac catctccgcc agctcgagcg cgcgaccaag cgactggccg gcatccacca
     1141 cctcctcgac gaggccgatc ttcaacgcgg ttgccgcatc cacccgctcg ccgcagagaa
     1201 tcatgcgttt cgcccatcct tcgccgacca tccaggccag gttctgggtt ccgcccccgc
     1261 acggcaacag acccaccgtg gcctcgggca acgccagctt cgcctgcgcc tccgcaacac
     1321 ggatgtcgca ggccagcgcg cactccagcc cgccgcccat ggcgaagccg ttcaccgcgg
     1381 cgatgctgac cccgcgaaac gcggacaacg tctcgaaagc cgagccgaac tggccggcca
     1441 tctccgaggc cactgccgga tccccgtctg caaacagctt cagatcggcc cccgccgaga
     1501 agaacttctc accctgaccg gtgatgacga tggcgtaaac gctgcgttct gcgttggcct
     1561 gctcgacgac gttcttgagc ccctgcagac tttccgcatc ccaggtattt gcgggcggcg
     1621 tgttgatggt gacgagcagc gtgtgccctc gctgctcgac cagcagcttt tcagtcggcg
     1681 attcaatcat cagcttcctc tcttgagtcg atcatcagtt cggtgttttc ttccagatag
     1741 tcgcatcacg caacgtcgaa ccagtctatc ccggccacgc cccgttgctg aaggtactcg
     1801 ttggcccggg caaaatgccc gcagccgaaa aaaccacggt gggcagacag cggcgaaggg
     1861 tggggcgcct tcaggacgca atgccgttgc acgtccagga tggctccttt cttctgcgcg
     1921 tagcttcccc acagcagaaa gaccagcccg tcccgctccc ggttgagaac gtcgaccacc
     1981 ttgtcggtaa agatctccca gcccatgccc tgatgcgagc cggcgcgacc acgctccacc
     2041 gtcagcaccg cgttgagcag cagcacgccc tgcctggccc acggctccag acagccgttg
     2101 gtgtcatcca gacgctttgc gggaccgccg agctcctcgt tgatctcctg aaagatgttg
     2161 acgagggatg gcggttgcgg gatgccgggc tgcaccgaga agcacaggcc gtgtgcctgg
     2221 tcggggccgt gatagggatc ctgaccgatg atcacgactt tgacgccctt ggaggacgtc
     2281 aggtccaggg cggcgaagat gttatcgccc cggggataga tcaccttgcc cgcccgcttc
     2341 tcccggagca ggaactcccg cagcttgttc atgtagggct tgtcgaactc accgccaagc
     2401 accgctttcc aggaaggctc caggcggacg cgctcctcgc tccggcgggc ggcctcaacc
     2461 atcttgcacc ggccgcatca gcggaaacag cagcacgtcc cggatggtcg gggagtccgt
     2521 gagcagcatc accagccgat cgatgcccag cccctcgccg gccgtgggcg gcagaccgta
     2581 ctcgagcgcg gtgatgtaat cgtcgtcgaa atgcatggcc tccacgtcgc cgccgcttcg
     2641 aagcagcgcc tgag
//
LOCUS       DBNHQR010000597         6363 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1768355, whole genome shotgun sequence.
ACCESSION   DBNHQR010000597 DBNHQR010000000
VERSION     DBNHQR010000597.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6363)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6363)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6363
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1768355"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..590
                     /locus_tag="AC16VR_13595"
     CDS             162..590
                     /locus_tag="AC16VR_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012238.1"
                     /translation="MNFQIEWSRRPWWMNLIWFFCLYMTFIYMPFDLFLKPVEIDEEV
                     WFGVTLKGWPAKLTAPLHWLVYGAGAYGFWKMSRWMWPWATVYVAQVSFSMLVWNLLD
                     HRGAGWLAGLLAAAVFAVPTVALWRARSIFRDAPEAAEPI"
     gene            complement(587..2458)
                     /locus_tag="AC16VR_13600"
     CDS             complement(587..2458)
                     /locus_tag="AC16VR_13600"
                     /inference="COORDINATES: protein motif:HMM:NF023649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HIR6012239.1"
                     /translation="MQRNFITLISALMALIGLLCNPGVADAKQLLWGDTHLHTSYSFD
                     AFLNNNLTADPDTAYRFAKGEPVIHPYNRARVQLQKPLDFLVVSDHAEFIGGMLHVYR
                     NGLDLDDAGIITQIEGWYRERQLREAIDAGEGAPVFRSLLPAGSDDPRTAAATFGQSQ
                     AQQLPVSPEVFRSAWQEIVDTAEAHYQPGKFTSLIGWEWSSVPGGANLHRIVVTDADA
                     QTAKTFQPFSSLESPYPDDLWAWLEKTSAATSAAFIAIPHNSNISKGWMFPRETLRGG
                     PVDAAYGTTRMRWEPLVEATQIKGDSETHPLLSPNDEFADFEEYSFYIEQQPGEYKPS
                     EGDYVRPALKTGLELGEETGVNPYQFGLIGSTDAHTGLSTAAEDNFWGKMATDSTPET
                     KINTLITGGSRGWTMSASGMAAVWAEENTREAIVDAMRRREVYATTGPRLRVRFFGGW
                     EYDQADLEVEDLAAAGSAKGVPMGGELTAAPENNGPRFMVVALKEPGGANLDRIQIVK
                     GWLNAEGEAEERVYDVVWSDGRQPDADGKLTPVGNTVDLMTVRYTNDIGAVELASVWQ
                     DPDFDPGQSAFYYARVLQIPTPRHAQYDARALGMDRPTIGPAVIQERAYTSPIWYAP"
     gene            complement(2549..2926)
                     /locus_tag="AC16VR_13605"
     CDS             complement(2549..2926)
                     /locus_tag="AC16VR_13605"
                     /inference="COORDINATES: protein motif:HMM:NF015953.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIR6012240.1"
                     /translation="MIGIILRILITALGLWAAASIIPGVQIDSTGTMVWAAIWLGVVN
                     AVIRPVAIILTLPLTLISLGGFLLVVNAAMFGLVAAMLNDFHVSGFFAALFGALIVSI
                     TSWLGSSFIGADGRYEVMVIRRD"
     gene            complement(2970..3395)
                     /locus_tag="AC16VR_13610"
     CDS             complement(2970..3395)
                     /locus_tag="AC16VR_13610"
                     /inference="COORDINATES: protein motif:HMM:NF042761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HIR6012241.1"
                     /translation="MSALLAGVIFGLGLSISGMADPTIVLAFLTLGPGWNASLLVVMA
                     AAVTVTTIGYRLAGRRPAPLLDSEFHAPARRDIDQRLVIGAVVFGVGWGLAGYCPGPA
                     IVGVLTLDPRALAFLPGFVVGMIAFEAVPKPSARQPEQA"
     gene            complement(3404..3805)
                     /locus_tag="AC16VR_13615"
     CDS             complement(3404..3805)
                     /locus_tag="AC16VR_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015095134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HIR6012242.1"
                     /translation="MTNAIIGGLLIGAAAVWLWISLGRIAGISGIAAQALTRPRQSLW
                     PLLFVAGLGIGGWLGQGVRHPEAIPELGVDSIVLLTLGGLLVGFGTRLGSGCTSGHGV
                     CGIARFSRRSLAATVIFVGMGMLTATLVHGT"
     gene            4065..5228
                     /locus_tag="AC16VR_13620"
     CDS             4065..5228
                     /locus_tag="AC16VR_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008568682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="steroid 3-ketoacyl-CoA thiolase"
                     /protein_id="HIR6012243.1"
                     /translation="MEDVYIVEAVRSPIGKRGKGLAGLMPADLLGAVQKAALERSGIA
                     PEKVGQVVGGCVSQVGEQSFNIARIAWLSQGLPEEVAATTVDSQCGSSQQATSLGAAM
                     VASGMENVVMCCGVEMMTRVPLGSNMKDGVPMGASYMEHYQPTSQFQGAAMIAEEYQL
                     TREDTDAFGLKSQQNAARAWQEGRFDREIAPVEAPVLDDSGQPTGETRVVERDEGPRD
                     TTREGLQKLEPVPGQPIHTAGTSSQVSDGAAVVILANRKAVDELGLTPRARIIATTLV
                     GVDPVTMLKGPIPASRKVLDQAGLGVDDIDVFEVNEAFASVVLAWQKELNPDPERVNP
                     NGGAIALGHPTGSTGARLITTALHELERSGGRYGLIAMCCGGGLGTGTIIERI"
     gene            complement(5232..5960)
                     /locus_tag="AC16VR_13625"
     CDS             complement(5232..5960)
                     /locus_tag="AC16VR_13625"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR6012244.1"
                     /translation="MFEEPNRRRIYLLRHAEAAYLDDDGNITDDVRTVGLTPTGRIQA
                     RKQAAVLASVEFDRAVCSGLPRTVETAKHILAYRDHPELETVSALEEIQGARGLAPQA
                     DVKAWLAMVANPWSGADHPEAQFLRGERFVDFAARVTPAFDAIVADHSWQTLLLVLHG
                     AVNRMLFNHIIGIPWSAKTSIEQDNCCINVIDVDTADDGATERFLIRGVNLTAYDLNK
                     SSFYLTHMEQAAQRMAGRVTGNAL"
     gene            complement(5953..>6363)
                     /locus_tag="AC16VR_13630"
     CDS             complement(5953..>6363)
                     /locus_tag="AC16VR_13630"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6012245.1"
                     /translation="QEINIGMAADVGTLQRLPRLIPDGLMRELAYTGRRMHADEALSC
                     GLVNAVYDSHEQMREAVGAIAAEIASKSPLAVTSTKHLLNYSRDHGVHDALAYQQLWM
                     GAVTQGGEMAEYFKAKADGRDPVYADLPPLEDDV"
BASE COUNT         1092 a         2004 c         2171 g         1096 t
ORIGIN      
        1 caggacacct tcaacctgcc caaggtgcag atcggcctgg aagccgccca gtacgaggag
       61 atcgtcctgg ccaactatca ggaaagcaag ctgcgccact tccacacgct gctggaggag
      121 tggatcgggc ggtagatccg taaccaggcg cggtgtttgt catgaacttc cagatcgaat
      181 ggtcgcgacg gccctggtgg atgaacctga tctggttctt ctgtctttac atgaccttca
      241 tctacatgcc cttcgatctg tttctcaagc ccgtggagat cgacgaggaa gtctggttcg
      301 gggtaactct gaaaggttgg ccagccaagc tcaccgcgcc cctgcattgg ctcgtttacg
      361 gcgccggcgc ctacggcttc tggaagatga gccgctggat gtggccctgg gcgaccgttt
      421 acgtggctca ggtgagcttc agcatgctgg tgtggaacct gctggatcat cgcggcgcag
      481 gctggttggc cggcctgttg gcggcagccg tcttcgcggt gccaaccgtt gctctctggc
      541 gcgcccggtc gatttttcgt gacgcgccgg aagctgctga gccgatctag ggcgcgtacc
      601 agatcggcga ggtgtaagcg cgctcctgaa tcaccgctgg ccctatggta ggccgatcca
      661 tgccgagcgc acgcgcatcg tactgtgcgt gccggggcgt agggatctgc agcacgcgag
      721 cgtagtagaa ggccgactgc cccgggtcga aatccggatc ctgccagacg gaggcaagct
      781 cgaccgcacc gatgtcgttg gtgtagcgga cggtcatcag atccacggtg ttgcctacgg
      841 gcgtcagctt gccgtccgcg tcgggctggc gaccgtcaga ccagaccacg tcgtagacgc
      901 gttcctcggc ttcgccctcg gcgttcagcc atcccttaac gatctgaatg cggtccaggt
      961 tggcgccgcc cggctccttg agcgctacca ccatgaaccg cggcccattg ttctctggcg
     1021 cagcggtcag ctccccgccc atgggaaccc ccttggcgga cccggcggcc gccaggtcct
     1081 cgacctcgag gtctgcctgg tcgtactccc agccgccgaa aaaccgcacg cgcaggcgcg
     1141 ggccggtggt ggcgtagact tcccggcgcc gcatggcgtc gacgatggcc tcgcgggtgt
     1201 tctcttcggc ccagaccgcg gccatgccgg aggcggacat ggtccaaccc cgggaaccgc
     1261 cggtgatcag cgtgttgatc ttggtctccg gcgtggagtc cgttgccatc ttgccccaga
     1321 agttgtcctc tgccgcggtg gaaaggcccg tgtgggcgtc ggtggagccg atcagcccga
     1381 actggtaggg gttcacgccg gtctcttcgc cgagctccag ccccgtcttc agcgcggggc
     1441 gtacgtagtc gccctcactg ggcttgtact cgcccggctg ctgctcgatg tagaagctgt
     1501 actcctcgaa gtcggcgaac tcgtcgttgg gcgagagcag agggtgggtc tccgagtcgc
     1561 ccttgatctg ggtggcttcc accaagggct cccagcgcat ccgggtggtg ccgtacgccg
     1621 cgtcaaccgg gcctccgcgc agggtttccc gggggaacat ccagcctttc gagatgttcg
     1681 agttgtgggg aatggcgatg aacgccgccg aggtcgcagc actcgtcttc tccagccacg
     1741 cccagaggtc gtccggataa gggctctcca gggagctgaa aggctgaaag gtctttgccg
     1801 tctgcgcatc ggcgtccgtc accacgatac ggtggaggtt ggcgccgccg ggcaccgagc
     1861 tccattccca gccgatcagg ctggtgaact tgcccggctg gtaatgtgcc tccgcggtgt
     1921 cgacgatctc ctgccaggcg ctgcggaaca cctcggggga tacgggcagc tgctgagcct
     1981 ggctctggcc gaacgtggcc gctgcggtgc gcggatcatc ggatccggct ggcagaagcg
     2041 agcggaaaac gggcgcgccc tcgccggcat caatggcctc ccgcaactgc cgttcgcggt
     2101 accagccctc gatctgcgtg atgatgcccg cgtcatccag gtccaggcca ttgcgataca
     2161 cgtggagcat cccgccgatg aactcggcgt ggtcggagac gacgaggaaa tcgagcggtt
     2221 tctgaagctg cacccgtgcc cggttgtagg gatgaatgac cggttcgccc ttcgcgaacc
     2281 ggtaggccgt atccgggtcg gccgtgaggt tgttgttcag gaacgcgtcg aacgagtacg
     2341 acgtatggag gtgggtgtcg ccccagagca gctgcttcgc gtcggccaca cctgggttgc
     2401 acaaaagccc gatcagtgcc atcagggcgg agatcagagt aatgaaattt cgctgcatcg
     2461 gctgaatctc tccgggaact tgagcatcat ctgaccgcgg cggattatcg gccaggggga
     2521 tgagccttgt cgaaccgggt ggcgcgggtc aatcccgtcg gatgaccatc acctcgtagc
     2581 gaccgtcggc cccgatgaag ctcgagccca gccagctggt gatgctcacg atgagcgctc
     2641 cgaacagcgc ggcgaagaaa ccgctgacgt gaaagtcatt cagcatggcc gccacgagac
     2701 cgaacatggc cgcgttgacg accagcagaa agccgccaag ggagatgagc gtcaggggca
     2761 gggtgaggat gatggccact ggccggatga cggcgttcac caccccgagc cagatggccg
     2821 cccagaccat ggttccagtg ctgtcgatct gcaccccggg aatgatgctg gcggccgccc
     2881 aaagaccgag ggcggtgatt aggatcctga gaatgatgcc aatcatggtc tcattctgcg
     2941 ccctacgggc ggcgacggcc agcgggcctt caggcctgtt ccggctgccg ggcggatggc
     3001 ttcggcacgg cctcgaaggc gatcatcccc accacgaagc caggcaggaa ggcgagcgcc
     3061 ctcgggtcca gcgtcaggac tccaacgatg gcgggacccg gacagtaacc ggccagcccc
     3121 cagccgacgc cgaacaccac ggcgccgatg acgagccgct ggtcgatgtc ccgccgcgcg
     3181 ggcgcgtgaa attcgctgtc gagcagtggt gccggtcgtc ttcccgccag ccggtagccg
     3241 atggtggtga ccgtcacggc ggccgccatg acgacgagca gcgaggcgtt ccaacccgga
     3301 cccagagtca ggaacgcgag cacgatggtg gggtccgcca tgccggagat gctcagaccc
     3361 agcccgaata tcactccggc caacagggct gacacgggcc ccatcatgtt ccgtgcacca
     3421 gggtagccgt cagcatgccc atgccgacga agatgacggt ggctgccagg gaacgtctgg
     3481 agaagcgggc gatcccgcaa acgccgtgac cgctggtgca gcccgaaccc aggcgggtgc
     3541 cgaagccgac cagaagcccg cccagtgtaa gcaggacgat gctgtcgacg ccgagttccg
     3601 ggatcgcctc ggggtgacgg acgccttgcc ccagccagcc gccgatgccc aggccggcga
     3661 cgaacaggag cggccagagg ctctgtcgcg gtctcgtcag cgcctgggcg gcaattccgc
     3721 tgatccctgc gatgcggccg aggctaatcc agagccacac ggccgcggcg ccgatgagca
     3781 gcccgccgat gattgcgttt gtcacgactt tccggtcggt tacgtgaggg tgggcgatct
     3841 tgccacgaag agcgcaacct gcctagttcc cggcgtaacc cgggatgagt cccggtagta
     3901 aagtgtggcc aggatctaga gaggatctgc ggagtgcaga cgactgacgg tgcggaaatt
     3961 ctgattgagg atcggggttc gcgaagctga gcgtccggaa acccgtggcg agctgccctg
     4021 gctcgggtag aatgcggcgc tctcgtccag ggagttcgta agcaatggaa gacgtctaca
     4081 tcgtcgaggc cgtccgcagc cccatcggca agcgtggtaa ggggcttgcc ggcctgatgc
     4141 cggccgatct gctcggagcc gttcagaagg cggcgctgga gcgcagcggc atcgcgccgg
     4201 agaaagtcgg gcaggtggtg ggcggctgcg tgtcccaggt gggcgagcag tcgttcaaca
     4261 tcgcccggat tgcctggctg tcccaggggc tgcccgagga agtggcggcc accaccgtcg
     4321 attcccagtg cggctccagc cagcaggcca cgtccctggg ggcggccatg gtggcctccg
     4381 gcatggagaa cgtggtcatg tgctgcggcg tggaaatgat gactcgggtc ccgctgggat
     4441 cgaacatgaa ggacggcgtg cccatggggg cgagctacat ggagcactac cagccaacca
     4501 gccagttcca gggcgcagcc atgattgccg aggagtacca gctcacccgt gaagatacgg
     4561 acgcgttcgg gctgaagagc cagcagaacg cggcccgtgc ctggcaggaa gggcgcttcg
     4621 accgcgagat cgcgcccgtc gaagcacccg tgctcgacga ttccggccag cccacgggag
     4681 aaacccgtgt ggtcgagcgc gacgagggcc ctcgcgatac cacgcgggag ggactgcaga
     4741 aactcgaacc ggtgcccggc cagccgattc acacggccgg gaccagctcc caggtctccg
     4801 acggcgcggc cgtggtgatt ctcgccaacc gcaaggcggt ggacgagctg ggtctcaccc
     4861 cgcgcgcccg gatcattgcc accaccttag tcggggtcga tccggtgacc atgctcaagg
     4921 gtccgatccc cgcatcgcgt aaggtgctgg atcaggcggg tctgggcgtg gacgacattg
     4981 acgtgttcga ggtcaacgaa gcatttgctt cggtggtgct ggcctggcag aaggagctca
     5041 atccggatcc cgagcgcgtg aaccccaatg gcggggccat tgcattaggc catcccaccg
     5101 gctccaccgg ggcgcgcctg atcaccaccg cgctgcacga gctcgagcgc agcggaggtc
     5161 gctacgggct gatcgccatg tgctgcggcg gcgggctggg cacgggaacc atcatcgagc
     5221 ggatctgaat ctcaaagcgc gttgccggtc acccggccgg ccatgcgctg ggccgcctgc
     5281 tccatgtggg tcaggtagaa gctcgacttg ttgaggtcgt aggccgtgag gttcacgccg
     5341 cggatgagaa accgctcggt ggcgccgtcg tccgcggtgt ccacgtcgat cacgttgatg
     5401 cagcagttgt cctgctcgat ggacgtcttc gcgctccacg gaatgccgat gatgtggttg
     5461 aagagcatgc gattgaccgc gccgtggagc acgagcagca gggtctgcca ggagtggtcg
     5521 gctacgatgg catcgaaagc gggggtgacc cgggcggcga agtcgacgaa gcgctccccg
     5581 cggaggaact gcgcttccgg atgatcggcg ccggaccagg gattggcgac cattgcgagc
     5641 cacgccttca cgtccgcctg gggcgccaga ccgcgggcgc cctggatttc ctctagagca
     5701 gagaccgtct ccagctctgg atgatcccgg tacgcgagga tgtgctttgc cgtctccacc
     5761 gtgcggggca gcccggaaca caccgcccgg tcgaactcca cgctcgcaag gacggccgcc
     5821 tgcttgcgtg cctgtatcct gccggtgggt gtcaggccga cggtgcggac gtcgtcggtg
     5881 atgttgccgt cgtcgtcgag gtaggccgct tccgcgtggc ggagcaggta gattcggcgg
     5941 cggttcggct cctcaaacat cgtcttccag cggcggcaga tcggcataaa ccggatcgcg
     6001 cccgtcggcc ttggccttga agtactcggc catctcgccg ccctgggtca cggcgcccat
     6061 ccacagctgc tggtaggcca gcgcgtcatg cacgccgtgg tcgcgcgagt agttgagcag
     6121 gtgcttggtg ctggtgaccg ccagcggaga tttgctggcg atctccgccg cgatggcacc
     6181 tacggcttcg cgcatctgct cgtgggaatc gtaaacggcg ttgacgagcc cgcaggaaag
     6241 cgcttcgtcc gcatgcatcc gccggcccgt gtaggcgagc tcccgcatga ggccatccgg
     6301 gatgagtcgg gggagccgct gcagggtgcc aacgtccgca gccatgccga tgttgatctc
     6361 ctg
//
LOCUS       DBNHQR010000598         1031 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1451479, whole genome shotgun sequence.
ACCESSION   DBNHQR010000598 DBNHQR010000000
VERSION     DBNHQR010000598.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1451479"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..948)
                     /gene="hisF"
                     /locus_tag="AC16VR_13635"
     CDS             complement(169..948)
                     /gene="hisF"
                     /locus_tag="AC16VR_13635"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HIR6012246.1"
                     /translation="MLSKRLIACLDVRDGKLAKSVKFVDTKDIGDPVEKAREYYLDGL
                     DELVFYDITASSDKRRIMLDVVEAVAEQVFIPLSVGGGVSSVGDATDLRLAGAEKINV
                     NSAAVRRPQLIAECAEAIGRQNVVLSMDIRRVEPCAELPSGYEIVIEGGRRPMAIDAL
                     EWALEGERLGAGELVINSIDADGTRDGYELTLTAMLAEAVRIPVIASGGAGKPEHLYD
                     ALTVGKADAALVASMVHYGDYKVSELKRYLDGRGVKMRMSW"
BASE COUNT          176 a          367 c          298 g          190 t
ORIGIN      
        1 cagatccgca cggttgacgg ccgtcgcgtg cacggcgatc agcacctcac cgaccccggc
       61 ctgaggctct tcggcctcct gccagctcag acgctctcct tcaatgatga cggctttcat
      121 tgctctccct gactggtaat ggcctttttc gctcgcccac gctgatgctc accaggacat
      181 ccgcatcttg accccgcggc cgtcgaggta ccgcttcagc tcgctcacct tgtaatcgcc
      241 gtagtgcacc atggaagcca ccagcgcagc gtccgctttc cccacggtca gcgcatcgta
      301 aaggtgctcg ggcttgcccg caccacccga ggcgataaca ggaattcgca cggcctccgc
      361 cagcatggca gtgagggtga gctcgtagcc gtcccgggtc ccgtcggcat cgatggagtt
      421 gatcaccagc tcgcccgcgc cgagccgctc cccttccagg gcccactcca gagcgtctat
      481 ggccatgggc cgacggccgc cctctatcac gatctcgtaa ccggaaggca gctccgcgca
      541 gggttccaca cgtcggatgt ccatggacaa gaccacgttc tgacgcccga tggcctccgc
      601 gcactcggcg atgagctgcg ggcgccgcac ggccgccgag ttgacgttga ttttttccgc
      661 cccggccagg cgcagatccg tggcatcccc caccgagctg accccgccgc caacagagag
      721 cggaatgaaa acctgctcgg ccacggcctc gacgacgtcg agcatgatgc gacgcttgtc
      781 gctcgatgcg gtgatgtcgt agaagaccag ctcgtcgagc ccgtccaggt agtactcgcg
      841 ggccttttcg accggatctc cgatgtcttt cgtgtcgacg aacttcacgc tctttgcgag
      901 cttgccgtcg cggacgtcga gacaggctat cagacgcttg ctcagcatcg gccgtcccag
      961 gcagcaaagc gcgcgagcag ccggagcccg acccggccgc ttttctccgg atgaaactgg
     1021 gtggcgaagt a
//
LOCUS       DBNHQR010000599         4519 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_565897, whole genome shotgun sequence.
ACCESSION   DBNHQR010000599 DBNHQR010000000
VERSION     DBNHQR010000599.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_565897"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..404)
                     /locus_tag="AC16VR_13640"
     CDS             complement(126..404)
                     /locus_tag="AC16VR_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007946969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2160 domain-containing protein"
                     /protein_id="HIR6012247.1"
                     /translation="MSFSWMAWTLPTAIFFGAILCLLVVMTVLEIRWPTRTRRGLLPL
                     ATTRGDRLFIGLLVAAFVHILWLAALDAPVLLATGISLGLLALLIRWG"
     gene            complement(424..1224)
                     /locus_tag="AC16VR_13645"
     CDS             complement(424..1224)
                     /locus_tag="AC16VR_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR6012248.1"
                     /translation="MTRRSHWLIFIYCAYLLLPVYWLVNMALRSNRDIVGGLEIVPGR
                     PTLANFAEIVTNPVWYEGFANSLTYVGVNTVISLLVALPAAFAFSRFRFLGDRHLFFW
                     LLTNRMAPPAVFLLPFFQLYSAVGLFDTPLAVALAHCLFTVPLAVWILEGFMSAVPRS
                     LDEMARIDGHSLPYFFFRIFLPVIRGGVGVTVFFCFMFSWVELLLARTLTASAAKPIV
                     VVMTRTVSASGMDWGVLAAAGVLTIVPGVAVVWFVRRHLVKGFALGRV"
     gene            complement(1221..2087)
                     /locus_tag="AC16VR_13650"
     CDS             complement(1221..2087)
                     /locus_tag="AC16VR_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR6012249.1"
                     /translation="MSGRRPDNRAWLGVLPVVLVVAFSAVIPLMTVVNYSLQDIFDQH
                     TRLFVGFDWYREILRDPRFLEALLRQLAFSGAVLLIEVPLGVMVARSLPRSGWLAPAL
                     VVLIALPLLVPWNVVGTIWQIFARPDIGLLGAALTGLGFEFNYTSSPVDAWITLLLMD
                     VWHWTSLVALLAYAGLASIQEAHYQAAEIDGAGRWQVFRLVELPRLRPVLTIAVLLRL
                     MDSFMIYTEPFVLTGGGPGNATTFLSQYLTSMAVGQFDLGPAAAFSVLYFLVTLLMCW
                     LFYTSVIRKEDE"
     gene            complement(2084..3190)
                     /locus_tag="AC16VR_13655"
     CDS             complement(2084..3190)
                     /locus_tag="AC16VR_13655"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6012250.1"
                     /translation="MSRIELDGVSHRYPEAPSETFAELELVIGSGEAHALLGSSGAGK
                     TTLLNLLSGIEQPTTGRITFDGRDVSRTPPRARNVAQVFQFPVLYESLSVAENLEFPL
                     KTRGVRAAERRRRAKAMAERLGIAELMGRRPGKLSLFERQLVAIGRALVRPELSLVLL
                     DEPLTAVEPSIKWQLRRELKAVQQEIGATMIYVTHDQTEALTFADRISLLHRGRILQT
                     GSARTLFSAPAHEQVGHFIGSPGMNLVPGEIAEGALRLGGRVVAPAAGAEPGPCTIGF
                     RPEWASVAPSGPDGSGLLGTITAARVLGVRRGVPVGILSVDLGVGVVQVRQALSASPG
                     ERVGVNVDAGQVVAFREGWRLPGIGAETGRGAGS"
     gene            complement(3177..4157)
                     /locus_tag="AC16VR_13660"
     CDS             complement(3177..4157)
                     /locus_tag="AC16VR_13660"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6012251.1"
                     /translation="MLKLQGIRLAEGAAPLDHEFRSGQITVVLGRNRSGKTRLCRLLA
                     GLEPTGEGRILLDGVDVSNIPAGQRPVSFVYQSFVNYPTLTVAENIASPMRARGLPSR
                     ERRRRVEDLAARLHISELLDRRPAQLSGGQQQRLAIARALAKQARVLVMDEPLVNLDF
                     KLREALELELRGLLRDAGIAVVYTSSDPRDAFNLGDEVLLLADHAPVQAGRPLDVYER
                     PVSVAAADLMSDPGVNLLPGPDRQAAIRPEHLHVSRHARDDETFAAELLTLETNGSET
                     YLHCRVADAHWVVKLEGLIDLKLRPGDTLPLFAEQRSLLRFSEAMTDVQD"
     gene            4264..>4519
                     /locus_tag="AC16VR_13665"
     CDS             4264..>4519
                     /locus_tag="AC16VR_13665"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6012252.1"
                     /translation="MDELFETYDEAGRAMGLMARHRVHATGAWHRSAHVFLFDTTGAL
                     LIQRRAPGKDLYPDLWDYSVGEHLVPGETYLAAARRGLEEE"
BASE COUNT          908 a         1542 c         1458 g          611 t
ORIGIN      
        1 cccatcgcaa ccggcgagca gcaaagtcgc cgccccaaga aaaagaacga tcccccgcat
       61 ccgcatcccc cctggccgaa cggcgcatac cttagcagga aacgccagga accgtgctcg
      121 accaatcagc cccagcggat gagcagcgcg agaagcccca ggctgatgcc ggtcgccaag
      181 agcacgggcg cgtcgagcgc ggccagccag aggatgtgaa cgaaggccgc caccagcaaa
      241 ccgatgaaca gacgatcacc gcgcgtggtt gccagcggca gaagaccccg gcgcgtcctc
      301 gtcggccatc gaatctcgag caccgtcatg accacgagca ggcagagaat cgcgccaaag
      361 aagatcgcgg tggggagcgt ccacgccatc caggaaaaac tcattgccct gcggcccccg
      421 ctttcacacc cgccccaggg cgaagccctt cacgagatgc cttctgacaa accacaccac
      481 ggcaacgccc gggacgatgg tcagcacgcc agcggccgcc agcacgcccc agtccatgcc
      541 ggaagcgctg accgttcggg tcatgaccac gacgatgggt ttcgccgcgc tcgctgtgag
      601 cgttcttgcc agcagcagct cgacccagct gaacatgaag cagaagaaca ccgtcacgcc
      661 aacgccgcct cggatgaccg gcagaaaaat tctgaagaag aagtagggga ggctgtggcc
      721 atcgatgcgc gccatctcgt ccaggctgcg ggggaccgcc gacatgaaac cttccagaat
      781 ccacaccgcc agaggcaccg tgaagaggca gtgggcgagc gccactgcca gcggcgtgtc
      841 gaagagcccg acggcggaat agagctgaaa gaacggcagc agaaagacgg caggcggagc
      901 catccggttg gtgagcagcc agaagaaaag atgcctgtcg cccagaaacc ggaagcgcga
      961 gaaagcgaat gcggctggca gcgccaccaa gagagaaatc accgtgttca ccccgacgta
     1021 cgtaagcgag ttggcaaacc cttcgtacca gaccgggttg gtcacgatct ccgcgaagtt
     1081 ggccagcgta ggccggccgg gaacgatctc cagcccgccc acgatgtccc ggttgctgcg
     1141 cagcgccatg ttgacaagcc agtaaacggg cagcagaaga tacgcgcagt atatgaagat
     1201 caaccagtgg ctgcggcggg tcattcgtct tccttcctga tcacgctggt atagaacagc
     1261 cagcacatca gcagggtgac gaggaagtag agaaccgaaa aagcggccgc cggccccaga
     1321 tcgaactggc ctaccgccat gctggtcagg tactggctga ggaaagtggt ggcgttcccc
     1381 gggccgccgc cggtcagcac gaaaggctcc gtgtagatca tgaagctgtc catcagccgc
     1441 agcagcacgg caatggtcag aacgggccta agcctcggca gctcgaccag ccggaagacc
     1501 tgccagcggc cggccccatc tatctcggcc gcctgataat gggcctcctg gatggacgca
     1561 agaccggcat aggccaggag cgccaccagg gacgtccagt gccagacgtc catgagcaga
     1621 agcgtgatcc aggcgtcgac ggggctggac gtgtagttga actcgaaacc caggccggtc
     1681 agcgcggcgc cgagcaggcc gatgtccggg cgggcaaaga tctgccagat ggttccgacg
     1741 acgttccaag ggaccagcag cggcaaagcg atcaggacga cgagcgccgg cgcgagccag
     1801 ccgctgcgcg gaaggctgcg tgccaccatg accccgagcg gaacctcgat gaggagcacc
     1861 gcgccgctga acgcaagttg tcgcaacagc gcctcgagga acctcgggtc gcgaaggatc
     1921 tctcgatacc agtcgaagcc gacgaacaga cgcgtgtgct gatcgaagat gtcctgaagg
     1981 gagtagttga ccaccgtcat gaggggaatc acggcgctga acgcgaccac cagcaccacc
     2041 gggagaacgc cgagccaggc ccggttgtcg ggccgacgtc cgctcacgaa cctgcccccc
     2101 gtccggtctc ggcaccgatt cccgggaggc gccagccttc ccggaaggcg acgacctgcc
     2161 cggcgtccac gttcacaccc acacgctccc ccggggaagc ggacaaagcc tggcgaacct
     2221 gcacaacgcc tacgccgagg tcgacgctga ggatcccgac gggcacccca cgtcgcacgc
     2281 cgagcacccg ggccgcggtg atcgtcccga gcaggcccga tccgtcaggc ccggacggcg
     2341 ccaccgatgc ccactccggg cggaagccga tggtgcacgg tccaggctcc gcacccgccg
     2401 ccggggccac cacgcgccct ccgagccgca acgcgccctc ggcgatctcg ccgggaacga
     2461 ggttcatccc cggcgagccg atgaagtgtc ccacctgctc gtgagcgggc gcgctgaaca
     2521 gggtacgggc cgagccggtc tgcaggatcc gcccgcggtg cagaaggctg atgcggtcgg
     2581 caaaggtaag cgcctcggtc tggtcgtgag tcacgtagat catggtggcg ccgatctcct
     2641 gctgcacggc cttcagctcc cgcctgagct gccacttgat cgaaggctcc accgccgtca
     2701 gcggctcgtc gagcagcacg agggacaact cgggcctgac cagcgcgcga ccgatggcca
     2761 cgagttgcct ttcgaacagg gacagcttgc cgggccggcg gcccatcagc tccgcgatgc
     2821 ccaggcgctc cgccatggct ttcgcgcggc gacgccgctc ggcggcgcgc acgccacggg
     2881 ttttcagggg aaattccagg ttctccgcca cgctgagaga ttcgtaaagc accggaaact
     2941 ggaacacctg ggccacgttg cgagcgcgcg gcggggtgcg cgagacgtcc cggccatcga
     3001 aggtgatacg cccggtcgtc ggctgctcga tgccggagag gaggttgagc agcgtcgtct
     3061 tgcccgcgcc cgagctgccc agcagcgcgt gggcttcgcc ggagccgatg accagctcga
     3121 gctcggcaaa cgtttcgctg ggcgcctccg gatatcggtg gctgacgccg tccagctcaa
     3181 tcctggacat cggtcatcgc ctccgagaat cgcagcaggg agcgctgctc ggcgaaaagc
     3241 ggcagcgtat cccccggccg gagcttcaga tcgatcaggc cctccagctt caccacccag
     3301 tgcgcgtccg ccacccggca gtggagatag gtctccgacc cgttggtctc gagggtgagg
     3361 agctcggcag caaacgtctc gtcatccctt gcgtggcggc tcacgtgcag atgctcggga
     3421 cgaatcgccg cctgccggtc aggcccgggc agcagattga caccggggtc gctcatcagg
     3481 tccgcggcag cgacgctgac cggacgctcg tagacgtcca gcggccggcc ggcctgcacg
     3541 ggtgcgtggt cggcaagcag gagcacctcg tcgcccaggt tgaaagcgtc ccgggggtcc
     3601 gagctcgtat agaccacggc gataccggca tcccggagca gcccgcgcag ctccagctcc
     3661 aacgcctcac gaagcttgaa atcaaggttc accagcggct cgtccatgac caggacgcgc
     3721 gcctgtttgg ccagcgcccg ggcgatcgcc agacgctgct gctggccacc ggacagctgg
     3781 gccgggcgcc ggtcgagcag ctccgaaatg tgcagccttg ccgccagatc ctcgacccgc
     3841 cggcgacgct cacgcgacgg caagccccgg gcccgcattg gcgaggcgat gttctccgcc
     3901 acggtgaggg tggggtaatt gacgaacgac tgatacacga aagagaccgg ccgttggccc
     3961 gccgggatat tcgagacgtc gaccccatcc agcaatatcc ggccctcacc cgtgggctcc
     4021 aggccggcca gcaatcggca caggcgcgtc ttgcccgagc ggttgcggcc gaggaccacg
     4081 gtaatctgcc ccgaccggaa ctcatggtcc aggggcgcgg cgccctcggc cagacggatt
     4141 ccctgcagct tcagcaccgc tgtcctggac ccgccggccg cctgcgtcgc tgggacatca
     4201 ttgacctcga cgtgcaaagg ccaaagtata gcgtccgcca tccccagcag aatcgaagga
     4261 ccattggacg agctgttcga aacctatgac gaggcgggca gggccatggg gctaatggca
     4321 cgccatcggg ttcacgctac cggtgcctgg caccgctctg cccacgtctt tcttttcgac
     4381 accacgggcg cgctgctcat ccaacgccgc gcgccgggca aggacctcta tccggatctg
     4441 tgggactaca gcgtcggcga gcacctggtg cccggcgaga catatctggc cgccgcccgg
     4501 cgcggcctag aggaggagc
//
LOCUS       DBNHQR010000600         3847 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1262594, whole genome shotgun sequence.
ACCESSION   DBNHQR010000600 DBNHQR010000000
VERSION     DBNHQR010000600.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1262594"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            254..2194
                     /locus_tag="AC16VR_13670"
     CDS             254..2194
                     /locus_tag="AC16VR_13670"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6012253.1"
                     /translation="MGQLKFDHLQPIVDDDATIEAALADAHIPSLMAAMVHVSGDPSW
                     IRGAIRPVNQFFGDSQGGIAPNDQAEVRRRALEALIAYRDGKATPPPPDPATVSEMVN
                     FVIGQEVTSDYGEFLHAELALDGADPYASEGFDEISEAARREFKVIIVGAGMSGLLAA
                     IRLKEAGISWEIVEKHADVGGTWYQNGYPGCRVDSPNHIYSYSFRPADWPQYYSSQEV
                     LREYFDKVATEYGLRDQIRFSTEVQSMAFDDASGTWQVEVTNGTGARETLTANAVISA
                     VGQLNRPKWPDLAGQDRFRGVSFHSTEWEHEHDLSGKRIIVIGTGASAFQFAPEIAKV
                     AAEVEIFQRTPNWMAPVVTYHDHIPLGKHWLLNHVPYYAKWYRFAMFWNMAEGVLSAV
                     RKDPAWNQTEDAISAENDTLRQMFTDWLEELCEGDEELLEKVVPKFPVGGKRVLLDNG
                     NWIRALRRDNVHLLTDPIAEINETGVVTEDGKQHDADVLIYGTGFQASRFLWPMKVTG
                     RDGIDLNEQWQGDARAYLGITIPNFPNLFCCYGPNTNIVVNGSIVFFSECEVRYILSC
                     IRQLLAGGHKALDCRADVHDAYNEWIDEGNANMAWGAARVPSWYKNEKGRVSQNWPFT
                     LLEYWNHTRDVNAADYEFLSDA"
     gene            2275..2904
                     /locus_tag="AC16VR_13675"
     CDS             2275..2904
                     /locus_tag="AC16VR_13675"
                     /inference="COORDINATES: protein motif:HMM:NF024660.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HIR6012254.1"
                     /translation="MILDLHTHSIKSDDGRAKVQNYCQWIRTREIPIDGLVLTEHRQF
                     DAESDYSPLAQQFGITILKGSEVETEYGHVLVFGVTEALTRAFDFADIHLPLARVIEA
                     CALHGAVPVPCHPGRRRVGMSAHLEEFGTPEGVRIVETHNGGSRGDEDAVAQAMAAEL
                     GYLGIGGSDAHIVSHVGRCATRFPGTISNEQELVEALLAEDFEAVTNRE"
     gene            2914..3357
                     /locus_tag="AC16VR_13680"
     CDS             2914..3357
                     /locus_tag="AC16VR_13680"
                     /inference="COORDINATES: protein motif:HMM:NF024844.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAS1-like dehydratase domain-containing protein"
                     /protein_id="HIR6012255.1"
                     /translation="MLDLEALRANFLNEDFDEQHFEVNAEQCVAYALACGETAPRYTD
                     PAHPDFQAPPTLPSSFHPGRRLPDGFPKLPGLGMDAGKAVAPKRPIRPGVPLVGRTHL
                     HDVYAKTGRSGRMTFMVTRMEIYDPDGEQLATADTRIVIRERPDQ"
     gene            3354..3767
                     /locus_tag="AC16VR_13685"
     CDS             3354..3767
                     /locus_tag="AC16VR_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR6012256.1"
                     /translation="MTVNVIGDVEFGEELPVFEPDTSLANVKRFTEAAGWQGPRFTDH
                     EAARREGLPGALVPGIMSQGFLAAMIHRWAPESQIESVDTVFRAPVLVDERHSISGVV
                     TDIDEDAGTVEIDLTVTNEANETRVFGTATVRLPA"
BASE COUNT          751 a         1271 c         1207 g          618 t
ORIGIN      
        1 ggctgtcggc gagccaggcg tgggaatatc cgagggcctc ggcgcgaacg acgtagtcga
       61 gatcgccgat gtgagaggcg acgcagattc cgatgtccat cctgatctcc gggatagtga
      121 acctggccga ttagcgtact taagggcgcc agcgcttgcc agacgatcct ggccgggacg
      181 ccccagccgt ccccttgcat ccgaacggct acctgcgcta aatggatcgg catatccagc
      241 cgggaaatca gccatggggc agctcaagtt cgaccacctg cagcccatcg tcgacgacga
      301 cgcgaccatc gaggccgccc tcgccgatgc ccacatcccg tcgctgatgg ccgccatggt
      361 gcacgtcagc ggtgatccca gctggatccg cggagccatt cgcccggtca accagttctt
      421 cggcgactcc cagggaggca tcgcgcccaa tgatcaggca gaggtccgca gacgggcgct
      481 cgaggcgctg attgcctatc gcgatggcaa agcgacgccc ccgcctcccg accccgcaac
      541 cgtcagcgag atggtgaact tcgtcatcgg ccaggaagtc acctcggact acggggagtt
      601 tctgcacgcc gagctcgccc tggacggcgc cgacccctac gcttcggagg gcttcgacga
      661 gatctccgag gcggcacggc gggagttcaa ggtcatcatc gtcggcgcag ggatgtcggg
      721 gctgctcgcg gcgatcaggc tcaaggaggc tggcatctcc tgggagatcg tggagaagca
      781 cgccgacgtg ggcgggacct ggtaccagaa cggctatccg gggtgtcggg tcgacagccc
      841 caatcacatc tactcctaca gcttccggcc cgccgactgg ccccagtact actcgagcca
      901 ggaagttctg cgggagtatt tcgacaaggt ggcgacggag tacggcctgc gcgaccagat
      961 ccgtttcagc acggaagtgc agtccatggc gttcgacgat gcgtccggga cgtggcaggt
     1021 ggaagtcacg aacggtaccg gcgccaggga gaccctcacc gcgaacgccg tcatcagcgc
     1081 cgtgggccag ctgaaccgac cgaaatggcc cgacctcgcc ggccaggatc gttttcgcgg
     1141 cgtgtccttt cactccaccg agtgggaaca cgagcacgat ctgtccggca agcgcatcat
     1201 cgtcataggc accggtgcca gcgcgtttca gttcgctccc gaaatcgcca aggtggcagc
     1261 cgaggtcgag atttttcagc gcacacccaa ctggatggcc ccggtggtca cctaccacga
     1321 ccacataccg ctggggaagc actggctcct caaccacgtt ccctactacg cgaagtggta
     1381 ccgcttcgcc atgttctgga acatggccga gggcgtgctc tccgcggtgc gcaaggatcc
     1441 ggcctggaac cagactgaag acgccatcag cgccgaaaac gacacgctcc ggcagatgtt
     1501 caccgactgg ctcgaagagc tgtgtgaagg cgacgaggaa ctgctcgaaa aggtggtgcc
     1561 caagttcccg gtgggcggca agcgggtgct gctcgacaac ggcaactgga ttcgcgcctt
     1621 gaggcgcgac aacgtgcacc tgctcaccga tcccatcgcc gagatcaacg agaccggcgt
     1681 cgtcaccgag gatggcaaac agcacgacgc cgacgtgctg atctacggca ccggcttcca
     1741 ggcaagccgg tttctatggc cgatgaaggt caccggtcgc gacgggatcg acctgaacga
     1801 gcagtggcag ggagacgccc gcgcctacct cggcatcacc atccccaact tccccaatct
     1861 tttctgctgc tacgggccca acaccaacat cgtcgtcaac ggcagcatcg tcttcttctc
     1921 cgaatgcgag gtgcgctaca tcctgagctg tatccgccag ctgctggccg gcggccacaa
     1981 ggccctggat tgccgcgcag acgttcacga tgcgtacaac gagtggatcg acgagggcaa
     2041 cgccaacatg gcctggggcg cggcccgggt accctcctgg tacaagaacg agaaaggccg
     2101 ggtgtcccag aactggccct tcacgctgct cgagtactgg aaccacacca gggacgtcaa
     2161 cgcggcggac tacgagttcc tctccgacgc gtgacgcgcc ccgcgctcgc agaagccggc
     2221 gcgcgacgtt aagattgcgc gctcgcggcg cttcgggtcg ttcaaggagg ttcggtgatt
     2281 ctcgatctgc acacacattc catcaagtcc gatgatggtc gtgccaaggt gcagaactac
     2341 tgccagtgga tccgcacacg cgagatcccc atcgacggcc tcgtgctcac cgagcatcgc
     2401 cagttcgacg ccgagtccga ctactccccg ctggctcagc agttcgggat cacaatcctc
     2461 aagggcagcg aggtcgagac cgagtacggc cacgtcctgg tgttcggcgt gaccgaagcg
     2521 ctgacccggg cgttcgattt cgcggacatc caccttcctt tggcccgggt catcgaagcc
     2581 tgtgcgctgc acggcgcggt tcccgttccc tgtcatcccg ggcgccggcg cgtgggcatg
     2641 agcgctcatc tcgaggaatt cggcacgccg gaaggggtga ggatcgtgga gacgcacaat
     2701 ggcggcagcc gtggagacga ggacgccgtg gcccaggcca tggccgccga gctcggctac
     2761 ctgggcattg gcggcagcga cgcgcacatc gtcagccacg tgggacgctg cgctacccgt
     2821 ttcccaggca cgatctccaa cgagcaggaa ctggtggaag cgctgctggc cgaagacttc
     2881 gaagccgtta ccaatcggga gtgaaagaca cccatgcttg acctagaggc gttacgggca
     2941 aacttcctca acgaagactt cgacgagcag cacttcgagg tgaacgccga gcagtgcgtt
     3001 gcctacgccc tggcctgcgg cgagacggcg ccgcgctaca ccgacccggc ccatcctgac
     3061 ttccaggcgc cgcccacgct gccgtcgagc tttcaccccg gccgccgcct gcctgacggc
     3121 tttcccaaac tacccggtct gggcatggac gcaggcaagg ctgttgcgcc gaagcggccc
     3181 atacggcccg gggtgccgct ggtcgggcgc acgcatctcc acgacgttta cgccaaaacc
     3241 ggacgatcgg gccgcatgac gttcatggtt acgcgcatgg agatctacga tcccgacggc
     3301 gagcagctgg caaccgctga cacccgaatc gtcattcgag agaggccgga tcaatgaccg
     3361 tcaacgtcat cggcgacgtc gagttcggcg aagagctacc ggtcttcgag ccggacacga
     3421 gcctggccaa cgtcaagcga ttcaccgaag ccgccggctg gcagggaccc cggttcacgg
     3481 accatgaggc cgccaggcgc gaggggctcc ccggagcgct ggtgccaggc atcatgagtc
     3541 agggttttct cgcggccatg atccatcgct gggcgccgga gagtcagatc gaaagcgtgg
     3601 acaccgtgtt cagggcgccc gtgctggttg acgaaaggca cagcatcagc ggcgtcgtca
     3661 ccgacatcga cgaagatgcg ggcacggtcg aaattgatct gaccgtgacg aacgaggcca
     3721 acgagacccg ggtcttcggc accgccaccg ttcgcctccc cgcctgatct cccgccggtt
     3781 ccgccgatca caaccggcaa aggcgacagc aaccggtcgt tcagatcggc tcgaaaaggg
     3841 gcacagc
//
LOCUS       DBNHQR010000601         3749 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_186505, whole genome shotgun sequence.
ACCESSION   DBNHQR010000601 DBNHQR010000000
VERSION     DBNHQR010000601.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_186505"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="AC16VR_13690"
     CDS             <1..637
                     /locus_tag="AC16VR_13690"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF016275.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HIR6012257.1"
                     /translation="IANHRVLMEGDVEAALQLMGAADVILEELDDFAFFQVRARLADE
                     MLALKGVRGGDLQGVYLRLEAIKTSIGELPVRLPDYFRPESSADASVEAEGFWSALAA
                     ELSGYFQLRRFDGATKPLMAPEENAYLELNLRLAMERAQLAVLRRNQLVFERSLSSAR
                     EWILELLDPDDPSVEQVAEELDALSTLKLERDLPDVSGSLTALLELRRGNP"
     gene            634..1848
                     /locus_tag="AC16VR_13695"
     CDS             634..1848
                     /locus_tag="AC16VR_13695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HIR6012258.1"
                     /translation="MKTGIVLIAIAAVLGGLVGTLVVRDPGYVLLAYDDMAVETSLWF
                     ALVALVVIYFVVRLLVFAFSRLAATGGSFGAWNRQRQSKKAREQTIRGLLLMAEGEWA
                     EARRLLVLAAPRVDSPLINNLSAARGAHATGDVEGRDELLRKAHQSTPGARFAVGLTQ
                     AQLQASAEQWEQCLASLLQLQSESPRHQLVLSMLAECYEALQDWRALIELLPGIRRAK
                     LRDKAQLDELQKRAWSRRLAATTEDLTGLWAQMPKELRRNPELVAVLCDRLLEAGLAN
                     DAEAAVRGALEHEWRSELVQRYGVIVSDDVGRQLIVAEGWLKSRPNDSALLLALGRIC
                     LMNEQWAKAREYLEASLRLRHTPEVYGELGRLCVAMGDVDRGSQYLKQSVIGLPDLPL
                     PEGEPGAAAGTG"
     gene            complement(1845..2507)
                     /locus_tag="AC16VR_13700"
     CDS             complement(1845..2507)
                     /locus_tag="AC16VR_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase domain-containing protein"
                     /protein_id="HIR6012259.1"
                     /translation="MQEIVLHVEPDFAFAAGQYLEILLPNGARIPLSIASGPYRLPDV
                     HLHYRSTPGIAEAVLFDELLQTSQTLDVAGPFGDVRLELPLAGPILIAAGGTGASQAL
                     GMLDALAQAAPRGEVPFEVSFLWCADREADLYRRDWLASLNADWLSTTLIADSRRDRD
                     NRALAWLRAQSARFRDRRIFLCGGPQFVYAAADALQAGGVPRGQLAADAFGYAPRPDN
                     SG"
     gene            complement(2555..>3749)
                     /gene="rho"
                     /locus_tag="AC16VR_13705"
     CDS             complement(2555..>3749)
                     /gene="rho"
                     /locus_tag="AC16VR_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HIR6012260.1"
                     /translation="ENLARSRKQEVIAAIVRKQAKEGEDIYGEGTLEILQDGFGFLRS
                     ADSSYLAGPDDIYVSPSQIRRFNLRTGDSIAGKIRPPKDGERYFALLKVDEINFSEPN
                     SKKHKILFENLTPLFPDDRLVLERGNGSTEDLTARIIDLIAPIGKGQRALIVSPPKAG
                     KTLVLQNIAQSIAANNPEVVLIVLLIDERPEEVTEMQRMVQGEVVASTFDEPPSRHVQ
                     VAEMVIEKAKRLVEHQKDVVILLDSITRLARAYNTIVPSSGKVLTGGVDAHALERPKR
                     FFGAARNIEEGGSLSIIATALVDTGSKMDEVIYEEFKGTGNQELHLERKIAEKRVYPA
                     INIRRSATRREELLMGEEELQRVWILRKLLHDMDDIAAIEFMIDKLKDTKTNEEFFAL
                     MRGGR"
BASE COUNT          597 a         1177 c         1280 g          695 t
ORIGIN      
        1 gattgccaac catcgggtgc tcatggaggg tgacgtggag gccgccctgc agctcatggg
       61 ggctgctgat gtcattctcg aggagctcga cgattttgcc ttctttcagg ttcgcgcccg
      121 gctggccgac gagatgctgg cgctcaaggg cgtccgcggc ggcgacctgc agggcgttta
      181 ccttcgcctg gaagccatta agaccagcat cggcgagttg cccgtccggc tgcccgacta
      241 cttccgaccg gagagctctg cagacgcgtc tgtggaggct gaaggcttct ggagcgcgct
      301 ggccgcggag ctttccggct attttcagct gcgccgattc gacggcgcga ccaagccgct
      361 catggcgccg gaagagaacg cttacttaga gctcaacctg cggctggcca tggagcgtgc
      421 ccaactcgcc gttctgcgcc gcaatcagct cgtcttcgag cggagcctct ccagcgcccg
      481 cgaatggatt ctcgagttac tcgaccctga cgatccgagc gtcgagcagg ttgccgagga
      541 gctggacgcg ctgagcacgc tcaagctcga gcgggatctg cccgacgtgt cgggctccct
      601 caccgcgctg ctcgagctcc gccggggcaa cccgtgaaga cgggcattgt gctgatcgcc
      661 atcgccgccg tgctgggcgg gctggtcggc acgctggtgg tgcgtgaccc ggggtacgtg
      721 ctgctggctt atgacgacat ggccgtcgag accagtctct ggttcgcgct tgttgctctg
      781 gtcgtcatct atttcgtcgt gcgtctgctt gttttcgcat tctcacggct ggccgccacg
      841 ggcggcagct tcggcgcatg gaaccgccag cggcagtcga agaaggcgcg tgaacagacg
      901 attcgcgggc tgctgctcat ggccgaggga gagtgggccg aagcgcggcg gctgctcgtt
      961 ctggccgccc cacgggtgga ttcgcccctg atcaacaact tgagcgccgc tcggggcgcc
     1021 cacgccacgg gcgacgtcga aggccgcgac gagctgcttc gcaaggccca ccagtcgaca
     1081 ccgggggcgc gattcgccgt ggggctcacc caggcgcagc tgcaggcttc ggctgagcag
     1141 tgggagcaat gcctggcttc gcttctccag ctgcagtccg agtcgccgcg tcatcagctg
     1201 gtgctgtcta tgcttgcgga gtgttacgag gcgctgcagg actggcgggc tctcatcgag
     1261 cttctgccgg gcatccggcg cgcaaagctg cgtgacaagg cccagctcga cgagctgcaa
     1321 aagcgcgcct ggagccggcg gctcgcggcg accacggaag acctcacggg tctttgggcg
     1381 cagatgccca aggagctgcg ccgtaacccg gagctcgttg ccgtgctctg cgaccggctg
     1441 cttgaagcag ggcttgcgaa cgatgccgag gccgcggttc ggggcgccct ggaacacgag
     1501 tggcgcagcg agctggtcca gcggtacggt gtgatcgtct cggatgatgt gggcaggcag
     1561 ctcatcgtgg ccgaggggtg gttgaagtcc cggcccaacg attcggcgct gctgctggcc
     1621 ctgggtcgaa tctgcctgat gaacgagcag tgggccaagg cccgcgagta cctcgaagcg
     1681 agcctgcgcc tgcggcacac gcctgaggtc tacggtgagc tgggccgcct gtgcgtcgcc
     1741 atgggcgacg tggaccgcgg cagccagtat ctcaagcagt cggtgatcgg gctgccggat
     1801 ctgccgctgc cggaaggcga gccaggcgcg gcggcgggga ccggctagcc cgagttgtcc
     1861 ggacggggcg cgtagccgaa cgcatcggcg gccaattgcc cgcggggcac accgccggct
     1921 tggagcgcgt cggccgctgc gtagacgaac tgcgggcccc cgcacaggaa gatccgccga
     1981 tcccggaacc gcgccgattg cgcccgcagc caggcaagcg cccgattgtc ccgatcccgt
     2041 ctcgaatccg cgatcagcgt ggtagacagc cagtccgcgt tcagcgaggc aagccagtcc
     2101 ctgcgataca gatcagcctc ccggtccgcg caccagagaa acgagacctc gaacgggacc
     2161 tcaccgcgcg gcgctgcctg cgcgagcgca tcgagcatgc cgagcgcctg gctcgcgccc
     2221 gttccccctg ccgcgatgag aatcgggccg gccagaggca actccagccg gacgtcgcca
     2281 aaagggccgg cgacatcgag ggtttggctc gtttggagca gctcatcgaa aaggacggcc
     2341 tcggcgatgc cgggggtgga tcggtaatgc aggtgcacgt cggggaggcg gtagggacct
     2401 gaggcgatgg aaagtggaat gcgcgcgccg ttcggaagaa gtatttccag gtactggccc
     2461 gcagcgaaag cgaagtctgg ctctacgtgc agtacgatct cctgcacggg tgcgcccgtg
     2521 cggcggacga tcctggcctg cacggttggc tcggtcagcg accgccgcgc atcagcgcga
     2581 agaactcctc gttggtcttc gtgtccttca gcttgtcgat catgaactcg atggcggcga
     2641 tgtcgtccat gtcgtggagc agcttgcgca ggatccagac ccgctgaagc tcttcctcgc
     2701 ccatcagcag ctcctcgcgg cgggtggccg agcggcggat gttgatggcc gggtagacgc
     2761 gcttttctgc gatcttgcgt tccaggtgaa gctcctggtt gcccgttccc ttgaactcct
     2821 cgtagatgac ctcgtccatc ttggagccgg tatccaccag ggcggtggcg atgatggaca
     2881 ggctgccgcc ctcttcgatg tttcgggccg caccgaagaa tctcttgggg cgctccagcg
     2941 cgtgagcgtc gacgccgcca gtcagaacct ttccggacga cggcactatg gtgttgtagg
     3001 cccttgcgag acgggtgatc gaatccagca gaatcacgac gtccttctgg tgctccacca
     3061 gccgctttgc cttctcgatg accatctccg ccacctggac gtgacgggag gggggctcgt
     3121 cgaacgtgct cgccaccacc tcgccctgaa ccatgcgctg catttccgtg acttcctcgg
     3181 gccgctcgtc gatgagcagc acgatcagca cgacctcggg gttgttggcc gcgatggact
     3241 gagcgatgtt ctgcagaacc agcgtctttc cggctttcgg gggagagacg atgagcgctc
     3301 gctggccttt gccgatgggc gcaatgaggt cgatgatgcg cgcggtcaga tcctcggtgc
     3361 tgccgttgcc gcgctcgagc accagccgat cgtcggggaa gagcggggtg aggttttcga
     3421 aaagaatctt gtgtttcttg gagttaggct cgctgaaatt gatctcgtcg accttcagca
     3481 gagcgaagta gcgctcgccg tccttgggcg gccggatctt gccggcaatg ctgtcgcccg
     3541 ttcgcaggtt gaatcggcgt atctggctag gcgagacgta gatgtcgtcg ggccccgcga
     3601 gataggaact gtctgcggat cgcagaaaac cgaagccgtc ctggagaatt tccagggtcc
     3661 cctccccgta gatgtcctcg ccctctttgg cttgcttgcg gacgatggcg gcgatgacct
     3721 cctgcttgcg cgaacgcgca aggttctcc
//
LOCUS       DBNHQR010000602         3497 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_122113, whole genome shotgun sequence.
ACCESSION   DBNHQR010000602 DBNHQR010000000
VERSION     DBNHQR010000602.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_122113"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="AC16VR_13710"
     CDS             complement(<1..265)
                     /locus_tag="AC16VR_13710"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6012261.1"
                     /translation="MDRLAGRTALITGAARGLGAQIAKRFAEEGAMVIVNDLNLEAAE
                     ATAAAIGGRALACDVSDSSAVNAMFEEVASIADRLDVLVNNAGI"
     gene            413..1105
                     /locus_tag="AC16VR_13715"
     CDS             413..1105
                     /locus_tag="AC16VR_13715"
                     /inference="COORDINATES: protein motif:HMM:NF018949.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0090471 - 9,15,9'-tri-cis-zeta-carotene
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0016120 - carotene biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HIR6012262.1"
                     /translation="MLQTLLAGLIFVGAHLGISSTSLRPRLVAAVGERAYQGIFSLVA
                     LVTISLLIWTYGEAPRYEYLWLPDPRLYWVTKVVMVVAFISMIGGFMVKNPTAVGMEG
                     ILKDPGQREHAATGMLRITRHPFQWSVVLWSASHIVANGDTVSVVFFAAFGVLSLLGT
                     LAIDRKKAAALGDDWEHFAAVTSNLPFAAVLSGRNRLVLKELVRPGAAGLVVYALVFW
                     GHQWVSGGVRLL"
     gene            complement(1102..1701)
                     /locus_tag="AC16VR_13720"
     CDS             complement(1102..1701)
                     /locus_tag="AC16VR_13720"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6012263.1"
                     /translation="MKIEIPGPAGPIEAELDDANDGSGSFAVLCHPHPQYGGTMHDAV
                     LSVLGRALAGAGVGCLRFNFRGVGGSAGAHDGKGGEVEDLLAVERWLRQEHSPAHLIL
                     GGYSFGAAVAWQALTSLAPPDQMILIAPPVSFMPSSRRPVGFRLDVFAGDADQFVDAA
                     ALNVLGDAGIHIIPGADHFFGGKWGDLAAGIEQVLQPAG"
     gene            1899..2810
                     /locus_tag="AC16VR_13725"
     CDS             1899..2810
                     /locus_tag="AC16VR_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6012264.1"
                     /translation="MIEADGLTRRYGDFVAVRNVSFSIEHGEIVGLLGHNGAGKTTIM
                     KMLTGYLEPSGGSVRVDGADIEQDRLRVQRELGYLPENLPLYPEMTVSDYLSYAATLR
                     GVDPRLAVARAVEETELEEKALDPISTLSRGFKQRVGVAQAILHEPRFVILDEPTNGL
                     DPSQTQHMRELIQRLAATATVVLSTHIMQEVDAICDRALILRDGRLVLDERLADLKRS
                     DQLRLRCAGGGPVADLLAGVSGVSRAESVAEGAWQLTVDGPLDAAAERLARALVDAGI
                     ALHELGPVQRDLETVFREVNEGVSDAA"
     gene            2800..>3497
                     /locus_tag="AC16VR_13730"
     CDS             2800..>3497
                     /locus_tag="AC16VR_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6012265.1"
                     /translation="MRPDVVKRIAGKELTLFFASPVGYLFLGAFLAFTLFVFFWVEAF
                     FARNVADVRPMFEWMPVLLVFLSAALTMRMWSEERRTGTLEFVATVPVSGWEFVVGKF
                     LACWVLLAIALLLTLPLPISVSLIADLDWGPVWAGYLAAMLLGGAYLAIGLFVSARTD
                     SQIVSLIVASFVCGIFYLLGSPVLTDLVGSTWADLLRSVGSGSRFESITRGVLDFRDL
                     YFYVSLLATFLALNV"
BASE COUNT          514 a         1105 c         1207 g          671 t
ORIGIN      
        1 tgatgccggc gttgttgacg agaacgtcga ggcggtcagc aatggaggcg acctcctcga
       61 acatcgcgtt gacggcggac gagtcgctca cgtcacaggc caacgcccgc cccccgatgg
      121 cggcagccgt ggcttcggcc gcttcgaggt tgagatcgtt cacgatcacc atggcgcctt
      181 cctccgcgaa ccgcttggcg atctgcgcgc cgagacccct ggcggcgccc gtgatcagcg
      241 ccgtcctgcc tgccagtctg tccaatgtct tccctccttc ccgggtgccc gacgatcgac
      301 cttgtcatca agtgtggccg aacacctcgg cgaagcgcaa cgagcaggca gcgcgaataa
      361 gccgtctttc gagtagtatc cgtccgaagc tggtcaggaa gacccagaga ccatgttgca
      421 gacgctgttg gccggcctga ttttcgtggg cgcccatctg ggtatcagca gcacgtcgct
      481 ccggccgcgg ctggtggccg cggtgggcga gcgtgcctac cagggaatat tctctctcgt
      541 tgccctcgtt acgatttctc tgctgatctg gacctacggg gaagctcccc gatacgagta
      601 cctgtggttg ccggacccgc gcctgtactg ggtgaccaag gtggtcatgg tcgtggcgtt
      661 catctccatg atcggcgggt tcatggtcaa gaatcccacg gccgtgggca tggagggtat
      721 cctgaaggat cccgggcagc gcgagcacgc ggccacgggc atgcttcgca tcacccgcca
      781 tccgtttcag tggagcgtcg tcctgtggtc ggcatcgcac atcgtcgcga acggagatac
      841 cgtgtcggtg gtgttcttcg ccgccttcgg cgtcctgagc ctgctgggga ccctggccat
      901 cgaccgcaag aaggcggccg cgctcggtga cgactgggag cactttgctg cggtcacctc
      961 gaacctgcct ttcgccgcgg tgctgtccgg ccgcaaccgg ctcgtgctca aggagctggt
     1021 gaggcccggt gccgcggggc tggtggtcta cgccctggtg ttctggggcc accagtgggt
     1081 ttcggggggc gtgcgcctgc tctagccggc cggctgcagc acctgttcga tcccggcggc
     1141 gagatcgccc cacttgccac cgaagaagtg atctgcgccg gggatgatgt ggatcccggc
     1201 atctccgagc acgttcagcg cggcggcgtc gacgaactgg tcggcgtcgc cggcgaaaac
     1261 gtccagcctg aagccgacgg gccggcggga tgaaggcatg aagctcacgg gaggggcgat
     1321 gagaatcatc tggtcgggtg gcgcgaggct ggtgagcgcc tgccaggcga ccgcggcacc
     1381 gaaggaatag ccgcccagga tcagatgagc ggggctgtgc tcctggcgca gccagcgctc
     1441 gacggcaagc agatcctcga cctcgccgcc cttgccgtcg tgggcgcccg cgctcccgcc
     1501 tacgccgcgg aaattgaagc gcaggcagcc tacgccggcg cccgcgagcg cgcggcccag
     1561 gaccgagaga acggcgtcgt gcatggtgcc gccgtactgc gggtgcgggt gacagagcac
     1621 ggcgaacgag ccggatccgt cgttggcatc gtcgagttcg gcctcgatgg gaccggccgg
     1681 tccgggaatc tcgatcttca ttggcagcgt ccagcgggcg ttcggatcac ggtaccagcg
     1741 gccaggtgcc ggcgtcagcc cttgaatttt tcggccattt ccctattttt cgcactcccg
     1801 ccagttcctt ggacgggcgc gcgggcgggg catcggggct gaacccggcc tcgaaatcgg
     1861 aatcgaaaaa agcggcaacg tgttggcagg ttgatctcat gatagaagca gacggattga
     1921 cgcggcggta cggcgacttc gtcgcggtgc ggaacgtgtc cttctccatc gagcacggtg
     1981 agatcgttgg cctgctcggg cacaacggcg cgggcaagac gacgatcatg aaaatgctca
     2041 cgggctatct cgagcccagc ggcgggtcgg tacgcgtgga cggcgccgac atcgagcagg
     2101 acaggcttcg cgttcagcgc gagcttggct acctgcccga gaacctgccg ctctacccgg
     2161 agatgacggt cagcgactat ctgtcctacg cggccaccct gcggggcgtg gatccgcgcc
     2221 tcgccgtcgc ccgggccgtg gaggagaccg agctcgaaga aaaggccctg gaccccatca
     2281 gcacgctttc gcgcggcttc aagcagcgcg tcggggtggc tcaggccatt cttcacgaac
     2341 cgaggttcgt gattctcgat gagcccacca acgggctcga tcccagccag acccagcaca
     2401 tgcgcgagct gatccagcgg ctggcggcca ccgcgacggt ggtgctctcg acgcacatca
     2461 tgcaggaggt ggacgcgatc tgtgatcgag cgctgatcct gcgcgacggc cgcctggtcc
     2521 tcgatgagcg cctggccgac ttgaagcgca gcgatcagct gcgcctgcgt tgcgccggcg
     2581 gcggccctgt ggccgatctg ctcgccggcg tttccggggt gagccgcgcg gaatcggtgg
     2641 cggaaggggc ctggcagctg accgtggacg ggccgctgga tgccgcggcg gagcgtctgg
     2701 cccgggcgct ggtggacgcc ggcatcgcgc ttcacgagct cggcccggtg cagcgggact
     2761 tggagaccgt gttccgggag gtcaacgagg gggtgagcga tgcggcctga tgtcgtcaag
     2821 cggatcgccg gcaaggagct gacgctcttc tttgcctcgc cggtcgggta cctgtttctc
     2881 ggtgctttcc tggccttcac gctgttcgtg ttcttctggg tggaggcttt cttcgcccgc
     2941 aacgtcgccg acgtgcggcc gatgttcgag tggatgcccg tgctgctggt tttcctttct
     3001 gcggcgctga cgatgcgcat gtggagcgaa gagcgccgga cggggacgct ggagttcgtc
     3061 gccacggtgc ccgtgagcgg ctgggagttc gtggtgggga agttcctggc gtgctgggtc
     3121 ctgcttgcca tcgccctgct gctgaccctg cctctgccca tatcggtctc gttgattgcc
     3181 gatctcgact ggggcccggt ctgggccggc tacctggctg ccatgctgct cggcggcgcc
     3241 tatctggcca tcgggctctt cgtcagcgcc cgcaccgaca gccagatcgt cagcctcatc
     3301 gtggcttcgt tcgtctgtgg cattttctac ttgctgggta gtccggtgct gaccgacctg
     3361 gtgggcagca catgggctga tctgctacgc agcgtcggta gcggttcgcg cttcgagtcc
     3421 atcacccgtg gggtgctgga ctttcgtgat ctgtattttt acgtgtccct gctggcgacc
     3481 tttctggcgc tgaatgt
//
LOCUS       DBNHQR010000603         4113 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1451913, whole genome shotgun sequence.
ACCESSION   DBNHQR010000603 DBNHQR010000000
VERSION     DBNHQR010000603.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1451913"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..1195
                     /gene="pdhA"
                     /locus_tag="AC16VR_13735"
     CDS             119..1195
                     /gene="pdhA"
                     /locus_tag="AC16VR_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HIR6012266.1"
                     /translation="MSDSLPFGVHPSTIIGPDGNLLRPIPEPLVDPATIRALYGWMHR
                     TRAFDAKAIELQRTGRLGTYASCLGQEAASVGIGHAMAPEDVLVPSFRDQGTQLYRGV
                     SMVELLLYWGGDERGSDYAVPRDDLPISITVGGHAPHAAGVALALKLRAEPRVAVCVF
                     GDGATSKGDVYEAMNIAGVWRLPVVFVVNNNQWAISTPRADQSAATTLAQKAVAAGFE
                     GSQVDGNDVLAVSEAVGDAIGHARKGDGPRLVECLTYRLGDHTTVDDASRYRSEAEVA
                     RWRQLDPLARVGAYLAQHEGWSDADEDALVERCDDEVASAATAYNGTTPQPPEAIFDH
                     LFASLPRELERQRATLLASAPADG"
     gene            1188..2168
                     /locus_tag="AC16VR_13740"
     CDS             1188..2168
                     /locus_tag="AC16VR_13740"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR6012267.1"
                     /translation="MAEHTLLEAVNLALRRAMADDDRVLVLGEDVGVVGGVFRATDGL
                     LQAFGPHRVFDTPLAETGIAGVAVGLAVQGFRPVAEIQFMGFIYAAIDQLANHASRMR
                     TRTRGRQSCPLVLRAPFGGGIHAPEHHSESAEAMLAHIPGLRVVIPSSPRTAYGLLLA
                     AIRDPDPVVFLEPKRLYRSAKEAFEDDGAALPLDRCHIVRPGKDLTLVTWGAMVQETL
                     AAAEQLSGEGVAAEVIDVATIKPLDGETICESVGRTGRLVIVHEAPITGGFGGEIAAC
                     VAERALYSLQAPILRVAGYDTVMPLARLEHHYMPSVSRIVGAARETLEAS"
     gene            2165..3271
                     /locus_tag="AC16VR_13745"
     CDS             2165..3271
                     /locus_tag="AC16VR_13745"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val family dehydrogenase"
                     /protein_id="HIR6012268.1"
                     /translation="MIATFDNVDELGPEKVIEIYDPSTGLKAIVVVDNVAAGPAIGGV
                     RMAVDATREECGRLARAMTLKNAMAGLAHGGAKSVIVADPAMADEAKEVLIRSFAHAI
                     ADLRDYIPGPDMGTDETSMALVRDVTGRSAGLPRELGGIPLDEIGATGLGLSVSLEVA
                     EAFTDLSLDGARIVVQGFGSVGQHAARFLARRGARLVGVSDSRGARAAAEGFDLDLLL
                     ASKQEGVSVGAFDEGEAIDPEDLVELDCDVWIPAARPDVLNGANAGRIRAKVVAQGAN
                     IPATPEAEQTLAARGVLVIPDFVANAGGVICAAVEYHGGSESQALATIQDKIHGNVKE
                     VLSRATTTGELPRKVAMDIARERVQAAMRYRKTY"
     gene            complement(3333..>4113)
                     /locus_tag="AC16VR_13750"
     CDS             complement(3333..>4113)
                     /locus_tag="AC16VR_13750"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6012269.1"
                     /translation="PPHRIAEFDRYLSTLVPLVRGEEAQMGPLTVGARGEAPIRHIMP
                     DKGFVNFDDRIPVYVSGFGPRSLGLAGKHGDGAVLAMPANPAVMANIWRMLEEGAAAA
                     GRTLDRSDFYTTGLTAIVVLDPGEASDSPRVRAECGAMAMASVHYAYDQWRNFGHQPP
                     GYLGGIWDDYCALLESYPQERRHQRIHAGHNCWVLPEEERFLTREVLETTCMIGTRDQ
                     LIDRLAALSEAGLDQVMILPNFDTRFDVLERVGRELVGRIP"
BASE COUNT          688 a         1394 c         1397 g          634 t
ORIGIN      
        1 ctgctcagct accgcaacgt cgactacaat gaggatgagc ttgaatcttt cgatgccgac
       61 gtgtgggagc tggccctgaa gttccgctgg tgacgcgcac gacgtgccgg agccgaccat
      121 gagcgacagc cttccattcg gagtccaccc cagcaccatc atcgggcccg acggcaatct
      181 cctgcggccg atcccggagc ccctggtgga cccggccacc atccgagccc tctacgggtg
      241 gatgcaccgc acccgcgcct tcgacgccaa ggccatcgag ctccagcgga ccggacgcct
      301 gggaacctac gcctcctgtc tcggccagga ggcggccagc gtcggcatcg gtcacgccat
      361 ggcgcccgag gacgtgctgg tgccttcgtt cagagatcag ggcacccagc tgtaccgggg
      421 tgtgagcatg gtcgagctgc tgctctactg gggcggcgac gagcgcggct ccgactacgc
      481 cgtgccgagg gacgatctgc ccatcagcat taccgtgggc ggccacgctc cccatgcggc
      541 cggcgtcgcg ctggcgctga agctccgagc cgaaccccgg gtggccgtgt gcgtgttcgg
      601 cgacggcgct acgtccaagg gcgatgtgta cgaggcgatg aacatcgccg gcgtctggcg
      661 gctgcccgtc gtcttcgtcg tgaacaacaa ccagtgggcc atctccacgc cccgagccga
      721 tcagtcggcg gccaccaccc tggcgcagaa agcggtggcc gccggtttcg agggcagcca
      781 ggtggacggc aacgatgtgc ttgccgtcag cgaggcggtg ggcgacgcca tcggccatgc
      841 ccgcaagggt gacgggccac gactggtcga atgcctgacc taccggctgg gcgatcacac
      901 caccgtggac gacgccagcc gctaccgaag cgaggccgag gtggcgcgct ggcggcagct
      961 ggatcccttg gcccgcgtgg gtgcctatct cgcccagcat gagggatgga gcgacgccga
     1021 cgaggatgcc ctggtcgagc ggtgcgacga cgaggtggcg tcggcagcca ccgcttacaa
     1081 cgggacgacg ccccagccac ccgaggccat cttcgaccat ctcttcgcca gccttccccg
     1141 ggagttggaa cgacagagag cgaccctgct agcaagtgcc ccggccgatg gctgagcaca
     1201 ccctgctgga ggcggtgaac ctggcgttgc gccgcgccat ggccgatgac gaccgtgttc
     1261 tcgttctcgg cgaggacgtg ggggtggtgg gcggcgtgtt cagggcaacg gacggcctgc
     1321 tgcaggcctt cgggccgcac cgggtcttcg atacccccct ggccgagacc ggcatcgccg
     1381 gcgtggccgt cggcctcgcc gtgcaggggt tccggcccgt ggcggagatc cagttcatgg
     1441 ggttcatcta cgccgcgatc gatcagctcg cgaaccacgc ttcgagaatg cggacccgca
     1501 cccgcgggcg ccagagctgc ccgctggtgc tgcgcgcgcc gttcggtggc ggcatccacg
     1561 cgccggaaca tcactccgag agcgccgagg ccatgctggc tcacattccg gggctgcggg
     1621 tggtcattcc ctcgtcgccg cggaccgcct acgggctgct tctggcagcc atccgggacc
     1681 cggacccggt ggtcttcctg gagccgaagc gcctgtaccg gtcggccaaa gaggctttcg
     1741 aagacgacgg cgcagccctc ccgctcgacc gttgccacat cgtccgtccc ggaaaggatc
     1801 tcaccctcgt cacctgggga gccatggtcc aggaaaccct ggccgccgcc gagcagctgt
     1861 ccggcgaagg cgtcgccgca gaggtcatcg acgtcgccac gatcaaaccc ctggacggcg
     1921 agacgatctg cgaatcggtg gggcgaacgg gccgtctcgt catcgtccac gaagccccga
     1981 tcacgggcgg tttcggcgga gaaattgccg cctgcgtggc cgaacgcgcg ctgtacagcc
     2041 tgcaggcacc catcctgagg gtggccggct acgacaccgt gatgccgctg gccaggctcg
     2101 aacatcatta catgccgagc gtgtcgagaa tcgtcggcgc cgctcgggaa acgctggagg
     2161 cttcatgatc gctactttcg acaacgtcga cgagctcggg cccgagaagg tcatcgagat
     2221 ttacgacccg agcacgggcc tgaaggccat cgtggtggtg gacaacgtcg ccgccgggcc
     2281 ggccatcggc ggggtcagga tggccgtcga cgccacgcgc gaggagtgtg ggcggcttgc
     2341 cagggcaatg acgctgaaga acgccatggc ggggcttgct cacggcggcg ccaagtctgt
     2401 gatcgtcgcc gacccggcca tggccgacga agcaaaggaa gtcctgattc gaagcttcgc
     2461 ccacgccatc gccgacctgc gagactacat acccggtccc gacatgggta cggacgagac
     2521 ctccatggca ctggtcaggg atgtcaccgg ccggtccgcc ggcctgccgc gggagctggg
     2581 gggcattccc ttggacgaga tcggcgcgac gggtcttggc ttgagcgttt ccctggaggt
     2641 cgccgaggcc tttacggatc tcagcctcga tggcgccagg atcgtggtcc agggattcgg
     2701 ctccgtgggc cagcacgcgg cccgctttct cgcccgacgc ggcgcccggc tggtgggcgt
     2761 ctccgacagc cggggagcgc gggcggccgc agagggtttc gatctcgatc tcctgctcgc
     2821 atccaagcag gagggcgtca gcgtcggcgc cttcgacgaa ggcgaggcca tcgatcccga
     2881 ggatctcgtc gagctcgatt gcgacgtgtg gattccggcc gcccggccgg acgtgctcaa
     2941 cggggcaaac gccggccgga tccgagccaa ggtagttgcc cagggagcga acatccccgc
     3001 caccccggag gcggagcaga ccctggcggc gcgcggcgtt ctggtgatcc cggacttcgt
     3061 cgccaacgcc ggcggggtca tctgcgcggc tgtggagtat cacggcggca gtgaaagcca
     3121 ggccctggcc acgatccagg acaagattca cggcaacgtc aaagaggtgc tctccagggc
     3181 gaccaccacg ggcgagctgc cgagaaaagt cgccatggac attgctcgcg aacgggtgca
     3241 agcggccatg cgctatcgta aaacctactg acccggagtg atgccccgga cggcagcgcc
     3301 tcgccccggc gaagacccag cgcgcccctg gctcagggaa tacggcccac cagctcccgg
     3361 ccgacccgct cgagcacgtc gaaccgggta tcgaaattgg gcaggatcat cacctgatcc
     3421 agacccgctt cgctgagggc ggcaagccgg tcgatgagct ggtcgcgagt gcctatcatg
     3481 caggtggtct cgagcacctc gcgggtcaga aaccgctcct cttccggcaa cacccagcag
     3541 ttgtggccgg catggatccg ctggtggcgc ctttcctgcg gataagattc gagcagagcg
     3601 cagtagtcgt cccatatgcc gccaagatag ccgggcggct ggtgaccgaa attgcgccac
     3661 tgatcgtagg cgtagtgaac ggaggccatg gccatggcgc cgcattcggc tcgcacccgg
     3721 ggcgaatcgc tcgcttcccc cgggtccagc acgacgatgg cggtcagtcc ggtcgtgtag
     3781 aagtctgatc gatccagcgt ccggcccgct gccgccgcgc cctcttccag catgcgccag
     3841 atgttggcca tcaccgccgg gttcgccggc atggccagca ccgcgccgtc gccgtgcttg
     3901 cccgccagac ccagagaccg cggtccgaag ccggaaacgt agacggggat tcgatcgtcg
     3961 aaattgacga accccttgtc cggcatgatg tgccgaatgg gcgcctcgcc gcgggcgccg
     4021 acggtcaaag gtcccatctg cgcctcctcg ccacggacca gcggcacgag ggtgctcaga
     4081 taccggtcga actcggcaat tcgatgaggc ggc
//
LOCUS       DBNHQR010000604         4257 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1010043, whole genome shotgun sequence.
ACCESSION   DBNHQR010000604 DBNHQR010000000
VERSION     DBNHQR010000604.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4257)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1010043"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="AC16VR_13755"
     CDS             complement(<1..471)
                     /locus_tag="AC16VR_13755"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014615.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HIR6012270.1"
                     /translation="MDWQAKLNEGGIVLLDGGTGSELQRRGVPMSGAAWSGAAAHTHP
                     DVLRAVHHDYIAAGAEVIITNTFGTSRATLRAAGLGDAFASINRRAVALAMEARELAG
                     RDVAIGGSISNLPPNMNIGDYPDPAEELADLRDQAAILAEGGVDFIALEMMQDVN"
     gene            566..1327
                     /locus_tag="AC16VR_13760"
     CDS             566..1327
                     /locus_tag="AC16VR_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012271.1"
                     /translation="MRTLVIQSASELDRPGWLSSCLQSVEGWARERGYDYRFRGDDAL
                     ELTPGWYRAKLIGRSPIVADLARLLLIRDALAKGYERACWIDADVLLYAPERLDLPDD
                     DECAFGREYWVQKNRDGRWQVRRNVHNALCSFVAGCPVLPFLIHATQRIISRADPQHI
                     APQMVGPKLLSALHNLTGFGLIDTIGALSPAVLDDVVQGGGEALRAMLAATPEPLAGV
                     NLCASVNGHRDLAALCRRLTDCSGLHLSGASHPAS"
     gene            1364..2683
                     /locus_tag="AC16VR_13765"
     CDS             1364..2683
                     /locus_tag="AC16VR_13765"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6012272.1"
                     /translation="MKEQSRYDHLREKAVLAPTTPTRRRQRIPFDPQLDPYEIPLQDL
                     NVANPELFRAEAFWPYFRRLREEAPVHYCSESQYGPYWSITRYDDIMQIEKNHAVFSS
                     EFSLGGVTLMGTPSSGNDVPMFIQMDPPKHDAQRKAVAPKFTQRNLGDLEATVRERAG
                     RILDGLPRNETFNWVEHVSIELTAQMLATLFGVPQEERHQLIRWSNIISNADNPEVVK
                     EKSSYFEALAQCGDYFDALWRDRKSSAPTGDLVSMLAHGPDTADMNSQELLGNAVLLT
                     VGGNDTTRNSISGGLLALNQFPEQYDKLLENADLVRTMVPEIIRWQTPLTHMRRTALA
                     DTEFRGQLIGKGDKVVMWYISGNRDPDAVERPDEFIIDRRRPREHLSFGFGIHRCLGN
                     RLAELQLRVLWEEILKRCPRIEVVGEPERASSVLFRAIQSLPVRIAA"
     gene            2756..3952
                     /locus_tag="AC16VR_13770"
     CDS             2756..3952
                     /locus_tag="AC16VR_13770"
                     /inference="COORDINATES: protein motif:HMM:NF047238.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase C-terminal domain-containing protein"
                     /protein_id="HIR6012273.1"
                     /translation="MAGQARSAAIVGIHEYPSRDVEGSVSPLQIKAESAARALADAGL
                     SWQDVDAIYDAGEAGMMSGLTIAEYLGLKPNVIDTTNVGGSSYEYHAAHAARDIAAGK
                     ARVALLTYGSTAHSNARAIGVGGRGGGGMHPADNMDAFAGMTLVANYAMVAQRHMYEY
                     GTTSEQLAEISVATRCHAMRNPEAVKAMEDLEFLDIRETTVEDVVGSRMIADPLHLLE
                     CCMISDGGGAVVIASPEVARNCARPPVWILGTGEATKYPENGGDITVSAAAQSGPQAF
                     AEAGVSPAEMDIAMIYDSFSITVLTILEDLGFCAKGEGGPWAAGGRLRFDRPADGPAL
                     NTDGGGLSSNHPGMRGIFLLIEAARQLRGDSCSQVDGAKLAVAHGNGGMLGSRHCAGT
                     VILGRD"
     gene            3964..>4257
                     /locus_tag="AC16VR_13775"
     CDS             3964..>4257
                     /locus_tag="AC16VR_13775"
                     /inference="COORDINATES: protein motif:HMM:NF013920.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HIR6012274.1"
                     /translation="MSEAKRPLPNLHEMDSRAFWEATREHELRYQECADCGTVVFYPR
                     RHCTGCLGDALAWKTAKGEGTVYTFSVIRQSYHPFFRALVPYAVAWIDLDEGPR"
BASE COUNT          739 a         1386 c         1417 g          715 t
ORIGIN      
        1 gttcacgtcc tgcatcatct ccagggcgat gaaatcgacg ccgccctccg ccaggatggc
       61 agcctgatcg cgcagatcgg ccagttcctc ggccgggtcg ggatagtccc cgatgttcat
      121 gttgggcggg aggttggata tcgacccgcc gatggcgacg tcgcgaccgg cgagctctct
      181 cgcttccatg gccagggcca cggcgcgccg gttgatggac gcgaacgcat cgcccaggcc
      241 ggcggctcga agggtggccc gggatgttcc gaaggtgttg gtgatgatca cctcagcgcc
      301 ggctgcaatg tagtcgtgat gcacggcgcg cagcacgtcg ggatgggtgt gcgcggccgc
      361 ccccgaccag gctgcgccgc tcatgggcac gcccctgcgc tggagctcgg agccggtgcc
      421 tccgtccagc aggacgatgc cgccctcgtt gagcttcgct tgccagtcca ctcgcttcac
      481 gtttctaatg agacgaaagg attttagctg acgtgagcgt ttccgtgccg gcagcaccca
      541 tcggccgcgt gcgcaactga tacgcgtgcg gacacttgtc atccagagcg ccagcgagct
      601 ggaccggccg ggttggctgt cgtcgtgcct ccaaagcgtg gaaggctggg cgcgggagcg
      661 tggctacgac tatcgcttcc gcggcgacga tgcgctggag ctcacgccgg ggtggtatcg
      721 cgccaagctc atcgggcgct ctcccatcgt cgcggacctg gcgaggctgc tgctgattcg
      781 cgacgcgttg gccaagggct atgagcgtgc ctgctggata gatgccgacg tgctcctgta
      841 cgcaccggaa cgcctcgacc tgcccgatga cgacgaatgc gccttcggcc gggagtactg
      901 ggtccagaag aatcgcgatg ggcggtggca ggtccgtcgc aacgtgcaca acgccttgtg
      961 ctccttcgtt gccggctgcc ccgttctgcc cttcctgatt cacgcgaccc agaggatcat
     1021 ctcccgtgcg gatccccagc acatcgcccc gcagatggtg ggccccaagc tgctttccgc
     1081 cctgcacaac ctgaccggat tcggcctgat cgataccatc ggtgctctgt ctcccgccgt
     1141 cctggacgac gtggtgcagg gcggaggaga agcgctgcga gccatgctcg cggccacccc
     1201 cgagcccctt gcaggcgtca atctctgcgc gtcggtgaac ggccaccggg acctcgccgc
     1261 gctttgccgc cgcctgaccg attgcagcgg gctccatctc tcgggcgcaa gccatccggc
     1321 ctcctgatat gatggcgggg tcggcgattg gtcagcgaag gtcatgaaag agcaatctcg
     1381 gtacgatcat ctgcgggaaa aggcggtgct ggcgccaacg acgcccacgc gacgccggca
     1441 gcggattccc ttcgatccgc agctcgaccc ctacgagatc ccgctccagg atctcaacgt
     1501 tgccaacccc gagctgttca gggcggaggc gttctggccc tacttccgac ggctgcggga
     1561 agaggcgccc gtgcactact gttccgaaag ccagtacgga ccgtactggt ccataacccg
     1621 ctacgacgac atcatgcaga tcgagaagaa ccacgcggtg ttctcctccg agttctcgct
     1681 gggcggggta acgctcatgg gcacgccgtc ctccggcaac gacgtgccga tgttcatcca
     1741 gatggatccg cccaaacacg acgcgcaacg caaagcggta gccccgaagt tcactcagcg
     1801 caaccttggc gacctcgagg ccaccgtgcg ggagcgcgcg ggcaggattc tcgacggcct
     1861 gccccgcaac gagacgttca actgggtgga gcacgtttcc atcgagctga ccgcgcagat
     1921 gctcgcaacg ctgttcggcg tgccccagga agagcgccat cagctgatcc gctggtcgaa
     1981 catcatcagc aacgcggaca accccgaggt ggtcaaggag aagtcgtcgt atttcgaagc
     2041 cctcgcgcag tgtggcgatt acttcgacgc cctctggcgc gaccgcaagt cgtcagcgcc
     2101 cacgggcgat ctggtgtcca tgctggccca cgggccggac actgccgaca tgaactccca
     2161 ggagctgctg ggcaacgcag tgctgctgac cgtgggcggc aacgacacca cccgcaactc
     2221 catcagcggc ggcctgctcg ccctgaacca gttcccggag cagtacgaca agctcctgga
     2281 gaacgccgat ctcgtccgca cgatggtgcc ggagatcatc cgctggcaga ccccgctcac
     2341 gcacatgcgc cgcacggcgc tggcggacac ggagtttcgt ggccagctga tcggcaaggg
     2401 tgacaaggtg gtcatgtggt acatatcggg gaaccgcgat ccggacgccg tggagcggcc
     2461 ggacgagttc atcatcgacc gcaggcggcc ccgtgagcac ctttccttcg ggttcggcat
     2521 ccaccgctgt ctggggaacc gtctggccga gcttcagctg agggtgctct gggaggaaat
     2581 cctcaaacga tgccctcgaa tcgaggttgt cggggagcct gagcgtgcca gctcggtgct
     2641 gttccgcgcc attcagagcc tgcccgtgcg catcgccgcc tagcccgcaa ggaccggcct
     2701 tggaccgtcg gcccgcacgg cttagactgc gggcatcaac ctgggggaga tagccgtggc
     2761 tggtcaagcc agaagcgccg ccatcgtcgg cattcacgag tacccttcgc gcgacgtcga
     2821 gggatccgtc agcccgttgc agatcaaggc ggaaagcgct gcccgggcgc tcgccgatgc
     2881 cgggctgagc tggcaggacg ttgacgccat ctacgacgcg ggtgaggccg ggatgatgag
     2941 cggtctcacc atcgccgagt acctcgggct taaacccaac gtcatcgaca ccaccaatgt
     3001 gggcggcagc tcctacgagt accacgccgc ccacgcggcc agggacatcg cggcgggcaa
     3061 ggcgagggtg gcgctgctga cctacggatc gacagcgcac agcaacgcac gggccatcgg
     3121 cgttggcggg cgcggtgggg gcggcatgca tccggcggac aacatggatg cctttgccgg
     3181 catgaccctg gtggccaact acgcgatggt ggcgcagcgg cacatgtatg agtacggcac
     3241 cacgagcgag cagctggcag aaatatccgt ggcgacccgc tgtcacgcca tgcgtaatcc
     3301 cgaggccgtc aaagccatgg aggatctcga gttcctcgac attcgcgaaa cgacggtgga
     3361 ggacgtggtc ggctcgagga tgatcgccga cccgctgcat ctgctggagt gctgcatgat
     3421 ctcggacggc ggcggagccg tggtcatcgc ctcgccggaa gtcgcccgta attgcgcgcg
     3481 tccgccggtg tggatcctgg gcaccggaga agccaccaaa taccctgaaa atggcggcga
     3541 cataacggtg agcgccgcgg cccagtccgg gccgcaggcc tttgctgagg cgggcgtctc
     3601 tccggccgag atggacatcg ccatgatcta cgattcgttc agcatcacgg tgctcacgat
     3661 cctggaggat ctgggattct gcgcgaaggg ggagggcggg ccctgggccg cgggagggcg
     3721 tttgcgcttc gatcgccccg cggatggccc ggccctgaac accgatggcg gcgggctctc
     3781 gtcgaaccat ccgggcatgc ggggcatatt tcttctgatc gaagccgcgc gccagctgcg
     3841 cggtgattcc tgctctcagg tggacggtgc gaagctggcc gtggcccacg gcaacggcgg
     3901 catgctcggc agccggcatt gcgcgggtac cgtgatactt gggcgggatt gaggagtagc
     3961 accatgagcg aagcgaagag accgctgccg aacctgcacg aaatggacag ccgggcattc
     4021 tgggaagcca cccgggaaca cgagctgcgc tatcaggagt gcgcggactg cggcaccgtc
     4081 gtcttctacc cgcggcggca ttgcacgggt tgtcttggcg acgcgctggc ctggaaaacg
     4141 gccaaggggg agggcaccgt ctacacgttc agcgtgatca ggcagagcta tcaccccttc
     4201 ttccgcgctc tggtccccta cgcggtggcc tggatcgacc tggacgaggg gccgcgg
//
LOCUS       DBNHQR010000605         6383 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_249033, whole genome shotgun sequence.
ACCESSION   DBNHQR010000605 DBNHQR010000000
VERSION     DBNHQR010000605.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6383)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6383)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6383
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_249033"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="AC16VR_13780"
     CDS             <1..1066
                     /locus_tag="AC16VR_13780"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6012275.1"
                     /translation="GPGLNYQERSGVLVSVDPPEHTFEVKIVASAFSVPYFESLIPEM
                     QAFVDEQIDGFHADGRVDLHQALSIALPLFVIFRMFGIPLRDEAGDKTEFVREMIIGS
                     VGLMLTPGHQVQAELAAGSVDEARMRAIAGTQQMFVDHLADCKRKLESGEYHDDSNIV
                     CRFLTTPGPDGTYLSDAKILGFCGFLLTAGSATTTIMLSNVVYRLLRQPEELAKLKAN
                     PELVPLAIEESLRLDAPVQGLFRTNDEETDLGPLHLEKDTKVMLLWAGANLDPTVFDD
                     PLRFSLDRDLAKVRRHLAFGYGTHFCRGAALARIEGEIFLRTVLERLPNLRLDGEVVM
                     EKRMPVLQGIRSLPLAWDVD"
     gene            1051..2529
                     /locus_tag="AC16VR_13785"
     CDS             1051..2529
                     /locus_tag="AC16VR_13785"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6012276.1"
                     /translation="MGRGLTRPMFARHRSLVDASGRIPSWRLGIFASIAFPSVGMTLP
                     LAIFLPPFYTKTLGLGLAEVGLVFMLVRLFDIVTDPVMGIIGDRFDSRWGRRRHWLVI
                     ALPFMMIGVYMAFMPTGSPSIWYLGFWLVVLYAGTTMKTISHTAWAAELSPDYDQRSR
                     IAGFNSFAAYMGSLLILGPLAFLEFRGTPPAGHEALTFFGTMALVFAPICVFAAVTLV
                     GERPTAPAPRIGLIRGLRMVLRNPHMRRLLLADAMAAIPGSVMSGLFIFYQAELLGNS
                     QFNSLALIGFFVAHIIGVPLWVRLSFRVGKHRAFGISSLCFCFTTSLFFFPGEGDVAL
                     FVAFLFFTGLTHSGLQFLLRSMGADVVDYDNVQTGGQRTGLYFALLSITAKAGGALAI
                     GITYPLLAVVGFDAQGGNSEDAKFAFRMIYLVVPVMAMILAYVFIRGFKLDEAEQRAL
                     QSRIAMRDGSADGEAGAAGSADVDGDGQRAIEGGSDDATRRG"
     gene            2507..4189
                     /locus_tag="AC16VR_13790"
     CDS             2507..4189
                     /locus_tag="AC16VR_13790"
                     /inference="COORDINATES: protein motif:HMM:NF013086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6012277.1"
                     /translation="MTQLDEADWDLTVDLVVVGSGAAGMACALTAKLEGLDVLMVEKT
                     DRYGGTTALSGGVLWVPNNSLMKQAGVEDSAERAVTYLKHNIGNRVSAARLEAFVENA
                     PKMLDFLRARDCLEVAVFEGFPDYRPEDPGGCQGGRSVEPKVFAGRRLGDALGDLRAR
                     AAMAPAGIVGTMTELRRLAAVRSNPLELLKAWKVFPRNLWNRLSGAHHLANGRALIAW
                     LRHAMERTDVPLWLNAPLARIVTDDDGAVRGVLVRRGEEPVRVEARRGVVITAGGFEH
                     NGEMRSEYFGENTAEYSSGSPGNTGDAIRAGLDAGAAVDLMDDAWWAPTFMPPGQGPQ
                     IVIFERGKPGNIIVNAAGQRFANEADPYNEFVKHMQEADRSGASAIPSFLIFDDRYRS
                     KYPLGGMLPGVTPRRYLENGFVVRADTLAELAEIMGIDPAGLEDTVARFNDMASKGSD
                     DDYHRGDSAFDRFAGDPGVRPNPCLAALDEPPFYGMRIYPGDLGTKGGLLTNEHAQVL
                     RGDGTVVRGLYAAGNSSASAMGNFYPGAGGTIGPAMTFGYIAARHAAAEGSQ"
     gene            4274..5287
                     /locus_tag="AC16VR_13795"
     CDS             4274..5287
                     /locus_tag="AC16VR_13795"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIR6012278.1"
                     /translation="MGGKTINRQWLINGNPRGRAVALTDFVENEAELAPLGEGDVRVK
                     VEYLSFDPSQKGQLENIAGYSRGGAMGEVMYARGIGEVVESRSPGVAEGTKVAGFVGW
                     QEYASLPARSLEIVPDDDLLTARLGPLGGTGLTAYFGLLRVGRPEPGDMVVVSGAAGA
                     VGSMVGQIAEIAGCHTVGIAGGPEKCAWLVDEVGYDAAIDYKNENIKARLKELCPDGI
                     NVFFDNVGGEALNDALARIAPHARVVICGGISRYEQETLPPGPANYFNVVFRQANIEG
                     FLLSGYEAEYDVARERITEWIRSGAIVYKEDVQHGFENIPATLLRLFSGKNFGKQLLR
                     MGD"
     gene            5397..6296
                     /locus_tag="AC16VR_13800"
     CDS             5397..6296
                     /locus_tag="AC16VR_13800"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6012279.1"
                     /translation="MSVSLRGKNAIVTGAAVGIGPAYAEALAAEGVNVAVCDIREEIL
                     ELPARLEAHGVRSVGWKADVSVPEDVRKVVDGTVEAFGGIDILISNAGKCWVSVADDD
                     LDKTIADYEGMVGTNLKGEYLMGRAVIAQMLEQGRGGEIVNVATDHMVTCGSPFEICP
                     KLETCPWADSPRPTGGGDVMDVYDASKWALNGFLFAWAKALRPHGIRVNGMCMGATDS
                     WMIRDFYGFPQTPGEETEEQMEEVSTWMAKGDTAQVVMNLLKEGPEGRTAQNINLCVG
                     RPTMLEPPLPNVYITEESLNVSA"
BASE COUNT         1043 a         2002 c         2196 g         1142 t
ORIGIN      
        1 cggccccggt ctcaactatc aggagcgctc gggcgtgctc gtcagcgtgg atccgccgga
       61 gcacaccttc gaggtgaaga tcgtcgccag cgctttctcg gtgccctact tcgagtccct
      121 catacccgag atgcaggcgt tcgtggatga gcagatcgac ggtttccatg ccgatgggcg
      181 ggtggatctg catcaggcgc tgtccatcgc gctgccgctg ttcgtgatct tccggatgtt
      241 cggcatcccg ctgcgcgacg aggccggcga caagacggag ttcgtgcgcg agatgatcat
      301 cggctccgtc gggctgatgc tgacgcccgg ccaccaggtg caggccgagc tcgccgccgg
      361 gtccgtggac gaggcccgaa tgagagccat tgccggtacc cagcagatgt tcgtagacca
      421 tctggcggac tgcaaacgca agctcgaatc cggcgagtac cacgatgaca gcaacatcgt
      481 ctgccgtttt ctgaccacgc cgggtccgga cggcacctac ctgtcggatg cgaagatcct
      541 cggtttctgc ggctttctgc tgacggcggg gagcgcgacg accacgatca tgctgtccaa
      601 cgtcgtctac cgcctgctca ggcaacccga ggagctcgcc aagctgaagg ctaaccccga
      661 gctcgtgccg cttgccatcg aagagtccct gcgcctcgac gcgcccgtgc agggcctgtt
      721 tcgcaccaac gacgaggaaa cggacttggg acctctgcac ctggagaagg acaccaaggt
      781 catgctgctg tgggcgggcg ccaacctcga cccgacggtc ttcgacgacc cgctgcgttt
      841 cagcctggac cgggatctcg ccaaggtccg ccgccatctg gccttcgggt acggaaccca
      901 cttctgccgc ggcgcggcgc ttgcccggat cgagggtgag atattcctgc gcacggtgct
      961 ggagcggctg cccaatctga ggctggacgg ggaggtggtc atggagaagc gtatgccggt
     1021 gctgcaggga atccgctccc tgccgctggc gtgggacgtg gactgacgcg gcccatgttc
     1081 gcccgccatc ggtctctcgt cgacgcttcc gggcgcatcc cctcctggcg gctgggcatc
     1141 ttcgcctcca tcgcgttccc cagcgtcggc atgacgctgc cgctggccat cttcctgccg
     1201 ccgttctaca caaagaccct gggcctgggc cttgccgagg tgggtctcgt gttcatgctc
     1261 gtgcgcctgt tcgacatcgt caccgacccg gtgatgggca tcatcggcga ccgcttcgac
     1321 agccgctggg gccggcgccg gcactggctg gtgatcgcgc tgcccttcat gatgatcggc
     1381 gtctacatgg cgttcatgcc gaccgggtcg ccctcgatct ggtatctggg cttctggctg
     1441 gtggtgctct atgcaggcac gaccatgaag accatctccc acacggcgtg ggcggcggag
     1501 ctgtcccccg attacgacca gcgatcgcgc atcgccggct tcaactcgtt tgcggcctac
     1561 atgggctcgc tgctcatcct gggccccctg gcgtttctcg aatttcgggg aacgccgccg
     1621 gcagggcacg aggcgttgac gttcttcggc accatggcgc tggtgttcgc gccaatctgc
     1681 gtgtttgcgg cggtcaccct ggttggcgag cgccccacgg cgccggcgcc tcgcatcggg
     1741 ctgattcgcg gcctgcgcat ggttctgcgg aacccccaca tgcgccgcct gctgcttgcc
     1801 gatgccatgg cagccattcc cggttcggtg atgtcggggc tgttcatctt ctatcaggcg
     1861 gagctgctgg ggaactccca gttcaactcg ctcgcgctca tcggcttctt cgtggctcac
     1921 atcatcggcg tgccgctctg ggtacgcctg tcgttcagag tcggcaagca ccgcgcgttc
     1981 ggcatcagct cgctgtgctt ctgcttcacc acgtcgctgt tcttcttccc gggcgagggc
     2041 gacgtcgcgc tgttcgtggc gtttctgttc ttcacgggtt tgacccattc gggcctgcag
     2101 tttctgctgc ggtccatggg ggcggacgtg gtggactacg acaacgtgca gacgggcggg
     2161 cagcgaacgg gcctttactt cgcgctgctc tccattaccg ccaaggcggg cggggcgctc
     2221 gccatcggca ttacctatcc gctgcttgcg gtggtgggtt tcgatgccca gggtggaaat
     2281 tcggaggacg cgaagttcgc tttccgtatg atctacctcg tggtgcccgt catggccatg
     2341 atcctcgcct acgtcttcat tcggggattc aagctcgacg aggcggagca gcgggcgctg
     2401 cagtcccgga tcgcgatgcg tgacggatcg gcggacgggg aagcaggggc ggccggatcg
     2461 gctgacgtcg acggggacgg ccagcgggcg atagagggag gcagcgatga cgcaactcga
     2521 cgaggctgac tgggacctca ccgtcgatct cgtggtggtg gggtccgggg cggcggggat
     2581 ggcctgtgcc ttgacggcaa agctcgaggg cctggatgtt ctgatggtcg agaagacgga
     2641 ccgttacggc ggcaccacgg cgctttccgg tggcgtgctg tgggtgccca acaactcgct
     2701 gatgaagcag gcgggcgtcg aggactcggc cgagcgtgcc gtgacgtatc tgaagcacaa
     2761 catcggcaac cgggtcagcg cggccaggct cgaggcgttc gttgagaacg cgccgaagat
     2821 gctcgacttt ctccgcgcgc gcgattgtct ggaagtggcc gttttcgaag gttttcccga
     2881 ttaccggccg gaagatccgg gcggctgtca gggcggccgg tcggtagagc cgaaggtctt
     2941 cgccggccgc aggctgggcg atgcgctggg cgacctgcgc gcccgcgccg ccatggcgcc
     3001 cgcaggcatc gtcggcacca tgacggagct gaggcgcctc gccgccgtgc gctccaaccc
     3061 gctggagctg ctcaaggcct ggaaagtctt cccgcggaac ctgtggaacc gtctctccgg
     3121 agcccatcac cttgccaacg ggcgcgccct catcgcatgg cttcgccacg ccatggaacg
     3181 cacggacgtt ccgctgtggc tgaacgcgcc gcttgcgcgc atcgtcaccg acgatgacgg
     3241 tgccgttcga ggcgtgctgg tcaggcgtgg cgaagagccc gtgcgggtgg aagccaggcg
     3301 cggcgtggtg atcacggcgg gagggttcga gcacaacggc gagatgcgct cggagtactt
     3361 cggcgagaac acggccgaat actcatccgg ctcgcctggc aacacgggag acgccatccg
     3421 cgcaggtctc gacgccggcg ccgcggtaga cctcatggac gacgcctggt gggcgcccac
     3481 cttcatgccg cctggccagg gaccgcagat cgtcattttc gagcgcggca agccgggtaa
     3541 catcatcgtg aacgcggcgg gccagcgctt tgccaacgag gccgatccct acaacgagtt
     3601 cgtcaagcac atgcaggagg cggatcgcag cggggcttcc gccatcccgt cgtttctcat
     3661 cttcgacgac cgttaccgga gcaagtatcc cctgggcggc atgctccctg gcgtcacgcc
     3721 tcggcgatat ctggaaaacg ggttcgtcgt gcgggcggac accctggccg agctggccga
     3781 aatcatgggg atcgacccgg ccggtctcga agatacggtg gccaggttca acgatatggc
     3841 ctcgaagggc agcgacgacg actatcaccg gggcgatagc gcgttcgacc gcttcgccgg
     3901 tgatcccggg gtacgaccca acccctgcct ggctgccctg gacgagccgc ccttctacgg
     3961 catgcggatt tacccgggcg atctcggtac caaaggcggg ttgctcacca acgagcacgc
     4021 tcaggttctg cgtggcgacg gcaccgtggt tcgcggcctg tatgccgcgg ggaacagctc
     4081 ggcctcggcc atgggtaact tctaccccgg tgccggcggg accatcgggc cggcgatgac
     4141 ctttggttac atcgccgccc ggcacgcggc cgccgagggt agccagtagg cggttaaagt
     4201 accatcgtga cggcgcgggg gcgctgcggg tggggcccgt cgcgcagacg gatttgatgt
     4261 caggggaaag caaatgggtg ggaagaccat caacagacag tggctcatca acggaaaccc
     4321 gcgcggcaga gccgtggcgc tgaccgactt cgtggagaac gaggcggagc ttgcgcccct
     4381 gggagaaggc gacgtccggg taaaggtgga gtacctgagc ttcgacccgt cccagaaggg
     4441 gcagctggag aacatcgccg gctattcccg gggaggcgcc atgggcgagg tgatgtatgc
     4501 caggggcatt ggcgaggtgg tggagtcgcg cagcccgggc gtggcggagg gcacgaaggt
     4561 tgccgggttc gtaggctggc aggagtacgc gtcgctgccg gctcgcagcc tggagatcgt
     4621 gcccgacgat gatctgctca ccgcccggct cggtccgctg ggcggcaccg gcctcaccgc
     4681 ctacttcggg ctgcttcggg tgggccggcc ggaacccggc gacatggtgg tggtgtcggg
     4741 tgcggccggg gccgtgggtt cgatggtcgg tcagatagcc gagattgccg gctgccacac
     4801 cgttgggatc gcgggcggcc cggaaaagtg cgcatggctc gtggacgagg tcggctacga
     4861 cgccgccatc gactacaaga acgagaacat caaagcccgc ctgaaggagc tgtgcccgga
     4921 cgggatcaac gttttcttcg acaacgtcgg cggggaggcg ctcaacgatg cgctggcgcg
     4981 gatcgcgccc cacgccagag tcgtcatctg cggcggcata tcccggtacg agcaggaaac
     5041 cctgccgccg ggtcctgcca actatttcaa cgtcgtgttc aggcaggcca acatcgaagg
     5101 ctttctgctg agcggctacg aagccgagta cgacgtcgcc agggaacgca tcaccgaatg
     5161 gatccgttcc ggggccatcg tatacaagga agacgtccag cacggcttcg agaacattcc
     5221 ggcgacgctg ctgcggctgt tctccggcaa gaacttcggc aagcagctgc tcaggatggg
     5281 cgactgaggt gccgtctccg gccggatcga tccccggttg gggcacgctg acgcagactg
     5341 aagccttcgt gtattctcgt ggcgggagtt caatcacatc tcgatgaggg gggatgatgt
     5401 cagtttcact gcgtggcaag aacgccatcg ttacgggcgc cgccgtcggc attggtccgg
     5461 cctatgcgga agcgcttgcc gctgaagggg tcaacgtcgc cgtttgcgac attcgtgaag
     5521 agattctcga gttgcccgcc cgcctggagg cccatggcgt ccggtccgta ggctggaagg
     5581 ccgacgtgtc cgtgcccgag gacgtgcgca aggtggtgga cgggacggtc gaagccttcg
     5641 gcggcatcga catcctgatc agcaacgccg gcaagtgctg ggtcagcgtg gccgacgacg
     5701 acctggacaa gacgatcgcc gactacgagg ggatggtggg gaccaacctc aaaggcgaat
     5761 acctcatggg cagagcggtg atcgcccaga tgctcgagca gggccgtggg ggcgagatcg
     5821 tcaacgtggc caccgaccac atggtcacct gcggctctcc gttcgagatc tgcccgaagc
     5881 tcgaaacctg tccctgggcg gattctcccc ggcccacggg cgggggtgat gtgatggacg
     5941 tctacgatgc gtccaagtgg gcgctgaacg gatttctatt cgcctgggcc aaggcgcttc
     6001 gtccccacgg tatccgcgtc aacggcatgt gcatgggcgc gaccgactcc tggatgatcc
     6061 gggacttcta cggcttcccg cagacgccgg gggaggagac cgaggagcag atggaggagg
     6121 tgtccacctg gatggccaag ggggatacgg cccaggtggt gatgaacctg ctcaaggaag
     6181 gcccagaggg ccgcacggcg cagaacatca acctctgcgt gggccggccg accatgctcg
     6241 agccgccgtt gcccaacgtc tacatcaccg aggagagtct caatgtcagc gcttgaggga
     6301 aaagtcgcca tcgtcagcgg cgccgcccag gggctcggcg aagccttcgc ccgggcgctg
     6361 agcgagcagg gggccatcgt cac
//
LOCUS       DBNHQR010000606         2180 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1198566, whole genome shotgun sequence.
ACCESSION   DBNHQR010000606 DBNHQR010000000
VERSION     DBNHQR010000606.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1198566"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="AC16VR_13805"
     CDS             complement(<1..432)
                     /locus_tag="AC16VR_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6012280.1"
                     /translation="MTVDVRQVGRVTVVTIDRPEVRNAVDGPTADALAEAFSAFDAND
                     ASDVAVLTGGDGVFCAGADLKAVAAGAGPRLRETGNGPLGCTRMLLSKPVIAAVEGFA
                     VAGGLELALWCDMRVAASDAVFGVYCRRWGVPLVDGGTIRLS"
     gene            complement(456..1721)
                     /locus_tag="AC16VR_13810"
     CDS             complement(456..1721)
                     /locus_tag="AC16VR_13810"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6012281.1"
                     /translation="MSGNRPTIDPAVFASAEYAADPYPTLKLLRDHYPVYNVPGTDHW
                     IITRYEDVVNGFRDTENFSASPNGESIGAVFGPTLMEYDGDDHTKLRNIVAPQFVGKK
                     LKALLPTVGRNAMALINAFTELHARRIAGQAAAVGEIDIVDDFATRLPLNVILDVLDL
                     PQEAHEMFHQWYPAMMNGINGGPELRALGVAANAEYHAYLDPLIDARSRNPADDLLSR
                     LAVAVVDGQRMTKEEIKSFASLILVAGAETTDKAIANLWYQLLANPEQWKAVQADPDL
                     LDQAFSEMMRIHPPASAQARRAKRDVELHGKVIPAGAYVQLSIYGANRDERVFRDPDR
                     FDLFREDLYFGKELRSGYHQQGMAGHLGFGLGKHFCVGYQLARAETVIGTRMLMEVIR
                     NPRFKTGSKPTPIAIRIEPWSLPLEFDVA"
     gene            1805..>2180
                     /locus_tag="AC16VR_13815"
     CDS             1805..>2180
                     /locus_tag="AC16VR_13815"
                     /inference="COORDINATES: protein motif:HMM:NF016211.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HIR6012282.1"
                     /translation="MRRKTTKLLFITGGWLSLALGIAGIPLPLLPTTPFLLLAAFCFA
                     EGSDRLHDWLVNHVHFGPPIRRWREHRAVSRRTKWMGSLSLLAVLAVSLGFGAPLWLL
                     AVQGCVVVGGGAFLWTLPEPPAE"
BASE COUNT          345 a          695 c          742 g          398 t
ORIGIN      
        1 ggacagccgg atggtgccgc cgtccaccag cggcacgccc cagcgccggc agtagacgcc
       61 gaagacggca tcggaggctg ccacccgcat gtcgcaccac agtgccagct ccaggccgcc
      121 ggccacggcg aacccctcga ccgccgcgat gacgggcttg gagagcagca tgcgcgtgca
      181 tccgaggggc ccgttgccgg tctcgcgcaa tcggggcccg gcgccggcgg cgacggcttt
      241 cagatctgcg cccgcgcaga aaacgccatc gcctccggtg agcacggcga cgtctgacgc
      301 gtcgttcgcg tcgaatgccg aaaacgcctc ggccagggcg tcggcggtgg gaccgtccac
      361 ggcgttgcga acttcggggc ggtcgatggt gaccacggtg acgcggccga cctgtctaac
      421 gtctacggtc atcactctcc ccctttccgg cggcttcagg ccacgtcgaa ttccaggggc
      481 aggctccagg gctcgatgcg gatggcgatg ggcgtgggtt tcgagccggt cttgaaacgg
      541 gggttgcgga tgacctccat gagcatgcgg gtgccgatga cggtctccgc ccgcgccagc
      601 tgatagccca cgcagaaatg cttgccgagg ccaaagccga gatgcccggc catgccctgc
      661 tggtggtagc cggagcgcag ctccttgccg aagtagagat cctcacgaaa cagatcgaaa
      721 cgatccggat ccctgaacac tcgctcgtcc cggttggccc cgtagatgga cagctgcacg
      781 tacgcgccgg cggggatcac cttgccgtgg agctccacgt cccgtttcgc ccgtcgcgcc
      841 tgggcgcttg ccgggggatg aatgcgcatc atctccgaga aggcctggtc cagcagatcg
      901 gggtctgcct gcacggcctt ccactgctcc gggttggcca gcagctggta ccacaggttg
      961 gcgatggcct tgtccgtggt ctccgcgccg gccaccagga tcaggctggc aaaggacttg
     1021 atctcctcct tggtcatcct ctggccgtcc accaccgcaa cggctaggcg cgagagcagg
     1081 tcgtcggcgg ggttgcgact acgggcatcg atgagcggat ctaagtaggc gtgatactcg
     1141 gcgttggccg ccacgccgag agcccgcagc tccggccccc cgttgatgcc gttcatcatg
     1201 gccggatacc actggtgaaa catctcgtgg gcttcctggg ggaggtcgag gacgtcgagg
     1261 atgacgttca gcggcagccg ggtggcaaag tcgtcgacga tgtcgatctc gcccacggcg
     1321 gcggcctggc ccgcgatccg gcgggcgtgc aactcggtga aggcattgat gagagccatg
     1381 gcgttgcgcc cgacggtggg cagcagggcc ttgagcttct tgccgacgaa ctgcggcgcg
     1441 acgatgttgc gcagcttggt gtggtcgtcg ccgtcgtact ccatgagcgt tgggccaaac
     1501 accgcgccga ttgattcccc gttgggagat gcggaaaagt tctcggtatc ccggaagccg
     1561 ttcaccacgt cctcgtagcg ggtgatgatc cagtgatcgg tgcctggaac gttatagacc
     1621 gggtagtgat cgcgcagcag cttcagcgtc gggtagggat ccgccgcgta ctccgcgctg
     1681 gcgaagacgg ctggatcgat ggttggccgg tttccgctca tggtggcgtc ctgttcatcg
     1741 caagattcga ttaggattga tgattgcaca aacagcggga ggctgcccca ggacctgttc
     1801 gagcatgcgc agaaaaacga cgaagctgct gttcatcact ggaggttggt tgagcctcgc
     1861 tctcggcatc gccgggatcc cgctgccgtt gctgcccacc acgcctttcc tgctgctggc
     1921 cgccttctgc ttcgccgaag gatccgatcg tctgcacgac tggctcgtca atcacgttca
     1981 cttcggaccg cccatccggc gctggcgcga acaccgcgcg gtttcccgcc gaaccaaatg
     2041 gatgggcagc ttgagtctgc tggccgttct ggccgtgagc ctcgggttcg gtgcgccgct
     2101 gtggctgctg gcggtgcagg gatgcgtcgt ggttggcggc ggggcgttcc tgtggaccct
     2161 tccggagccg ccggcagagg
//
LOCUS       DBNHQR010000607         6541 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1643362, whole genome shotgun sequence.
ACCESSION   DBNHQR010000607 DBNHQR010000000
VERSION     DBNHQR010000607.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6541)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6541)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6541
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1643362"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..535)
                     /locus_tag="AC16VR_13820"
     CDS             complement(14..535)
                     /locus_tag="AC16VR_13820"
                     /inference="COORDINATES: protein motif:HMM:NF025050.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6012283.1"
                     /translation="MSIIRHPIWQYAYFVNDIDEACHKWNRMVGAGPFHVVRHHIAEG
                     FLYRGEHVEADVSYAFGQAGPAHIQFIAQHDDTPSIYRDMYAPGEQGFHHIGLLVHDV
                     DGEIKRFNEAGYETACTLWGGDYVAYMDCRQDMGCYVELHGDAPIIRDLFDGWKRQHE
                     EWDGVTDLIREQG"
     gene            651..830
                     /locus_tag="AC16VR_13825"
     CDS             651..830
                     /locus_tag="AC16VR_13825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012284.1"
                     /translation="MSVQNGERLIRFLRSDPQLREKVKDAGWEKFQDVTAEAGASCTT
                     YEVVAALIREIENDS"
     gene            847..1188
                     /locus_tag="AC16VR_13830"
     CDS             847..1188
                     /locus_tag="AC16VR_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012285.1"
                     /translation="MGNKLILVGLVEPQTDDLVEAFNQWYLGNHVEDTYNCPNITSVR
                     CFKAVRGFLGEPPSGYLTIYEFEGEDAEEAERILGQYQSDPDAWPEREPNNGSMKVVG
                     AGWYAEEIAFG"
     gene            1212..2039
                     /locus_tag="AC16VR_13835"
     CDS             1212..2039
                     /locus_tag="AC16VR_13835"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6012286.1"
                     /translation="MPHVRINGARLWYEVRGSGEPLLLHHGYTASRVNWLPVAERLQD
                     RYQVVLMECRGTGDSEHTAEGYSLAQYAADVVGMLDHLDLERVTFAGHSMGGGIGYLL
                     GLDHAHRLDRLVLMAPIPAAGIAGLDPDARQKRLKLRRDQNRDALLAEYRAARFRPDV
                     ETDDWIESRVDHVLGVSDGHYEASAESMVELDVGHRLPELEVPTLMLAGAADELLRAN
                     LHDFLALPNATLEVLSRAGHEVAVHEPDAVARAIDAFMTHGPVTARHIRQQMEGESR"
     gene            2036..2935
                     /locus_tag="AC16VR_13840"
     CDS             2036..2935
                     /locus_tag="AC16VR_13840"
                     /inference="COORDINATES: protein motif:HMM:NF023568.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6012287.1"
                     /translation="MSAPPTRRRWQVADAFGDGNIEIAGLDWRGSGPLALLHHANGFC
                     AATWTLVARGLAGSHRVIAIDARGHGDSGTRPIPEGYQWDFFASDLTQVARQLLEETG
                     QPSVEFGIGSSMGGIVTAASEARNPGLFQRIAMLDPPLHPLPELRARLGLGVAAPNAD
                     LVAMTRKRKTIWPSRAIARQAWQDKPLFREWVPAAFDAYLEEGLGDRPDGTVALKCDP
                     GVEAAIFETTGSMDTFELAPRVSAPVLLVRALQGRAPGAAFEHLAGLLPRCTYREVDA
                     GHLLPMESPELTLALLAEFTEGG"
     gene            complement(2966..5119)
                     /locus_tag="AC16VR_13845"
     CDS             complement(2966..5119)
                     /locus_tag="AC16VR_13845"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HIR6012288.1"
                     /translation="MNTSASDAAARSLAVIEETRQHGSIGHLPVWENITAAHVEAGVR
                     ALLDESEEAFTALETDVEASWAGLMEPLERLQTRIGQVIGAVTHLISVKYSDELQAAY
                     DAIRPIYVSLLNRMSQSLGVYGGLVALRDGPDWPALSQARQRIVTESIRAMERSGVHL
                     EGPPKERFKEIQERLSELGNDFSVNLVKEEKASRVRVQAPEEVAGVPAAVLGLAAAKA
                     REDGEDGATDASGPWHFVVNGVNFVAVMEHALDRGLRERFYRAFRARGTSRDFDNRCV
                     LTEILRLRQEQSALVGFECYADRSLDAKMAPDTDAVWQLFNELEAAARPAAEREFETL
                     KAYMRDEGGLDEGEQPAPWDLAFWSEKMRESLYAYDGERLREYFQLPRVLDGLFSLVG
                     RLYEVEVRRVAAGAVPVWDPAVEFFEVIGAGEVIAGFYVDPYARPGEKRGGAWMNTVV
                     SRSRVLAAPGKSSSLPVALFVMNARPPAEGKVALLSLDEVRTLFHEFGHATQHMFTTI
                     EDGGASGMNLVEWDAVELASQFNEYWMEHKPFLRDLTAHFDTGEPLDDETLDRIVDSR
                     NFMVGTATLRQLFFGKVDMTLHQRFGVAGSNDGASPFEIERDIADSTLVTPRLDDEST
                     LPAFGHLFAGGYAAGYYSYKWAEVLAADAFAAFKEAGLDDGAAMKEIAGRFRDTVLGL
                     GGSLPAGDVYRRFRGRDASVRALLADQGLVAAEAG"
     gene            complement(5247..6116)
                     /locus_tag="AC16VR_13850"
     CDS             complement(5247..6116)
                     /locus_tag="AC16VR_13850"
                     /inference="COORDINATES: protein motif:HMM:NF014045.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HIR6012289.1"
                     /translation="MARENEDRNLALFCDFENVALGVRDAKYARFDIQLVLERLLVKG
                     SIVMKKAYCDWDRYKEFKPVMHEAAFELIEIPHTRQSGKNSADIRMVVDALDLCYTKE
                     HIDTFVIISGDSDFSPLVSKLRENAKTVIGIGVKNSTSDLLIGNCDEFIFYDDLVRHQ
                     KATRKPRKKPAAKKAPEKKDATDAMDGKDAKEAKRAEPARSEEGNTDEGIALVLETAE
                     ALYGERGEREKLWGSMVKQTLKRRRPGFNESFYGFRSFSDLLEEAESRGLLELEMDER
                     SGGYLIRRLTREE"
BASE COUNT         1155 a         2205 c         2065 g         1116 t
ORIGIN      
        1 atctccatga cgctcagccc tgctcgcgaa tcagatccgt gacgccgtcc cactcctcgt
       61 gctggcgctt ccagccatcg aagagatcgc gaatgatagg cgcatcaccg tgcagctcca
      121 cgtagcaacc catgtcctga cggcaatcca tataggccac gtagtcgccg ccccagaggg
      181 tgcaggcggt ctcgtagccc gcctcgttga accgcttgat ctccccgtcg acgtcgtgca
      241 cgagcagccc gatgtggtga aagccctgct cgccgggcgc gtacatgtcc cggtagatcg
      301 agggcgtgtc gtcgtgctga gcaatgaact gaatgtgtgc agggccggcc tggccgaacg
      361 cgtagctcac gtccgcctcg acgtgctcgc cgcgatacag gaaaccttcg gcgatatgat
      421 gccgcacgac gtggaacggt cccgcgccca ccatgcgatt ccacttgtga caggcttcat
      481 cgatgtcgtt gacgaagtaa gcgtactgcc agatgggatg ccggataatg ctcatgatgt
      541 ctcccctttg aaatccacct caaaccttgc cgaacgatag cagactaacc cgacgccgcc
      601 gagtgctgta gactcggcta cgaaaccgac atctcgaaaa ggggggggac atgtccgtac
      661 agaacggcga gcggctcatt cgtttcctta gaagcgaccc gcagctgcgg gaaaaggtca
      721 aggacgctgg atgggaaaag tttcaggacg tcaccgccga ggccggtgcc tcctgcacga
      781 cctacgaggt cgtcgccgcg ctcattcgcg agatcgagaa cgacagctga acaggaaaga
      841 cgattcatgg gcaacaaact gatcctcgtg ggcctggtag agccgcagac ggatgacctg
      901 gtcgaggcct tcaatcagtg gtacctgggc aaccacgtgg aagacaccta taactgcccg
      961 aacatcacgt cggtgcgctg cttcaaggcc gtgcgggggt tcctgggcga gccgccctcc
     1021 gggtacctga ccatctacga gttcgaaggc gaggacgccg aggaggcgga acgcattctc
     1081 ggtcagtatc agtccgatcc cgacgcctgg cccgagcgcg agccgaacaa cggatcgatg
     1141 aaggtcgtgg gcgcaggctg gtacgcagag gagatcgcct tcggctgagc ccgagcgcgg
     1201 cgaccgtaaa aatgccccat gtccgcatca acggggcccg tctgtggtac gaggtccgag
     1261 gctccggcga gcccctgctg ctgcatcatg gctacacggc gtcacgggtc aactggctgc
     1321 ccgtggccga gcgtctgcag gaccgctatc aggtcgttct catggagtgc cggggtaccg
     1381 gagatagcga gcacaccgct gagggataca gcctcgccca gtatgcggcc gacgtcgtcg
     1441 gcatgctgga ccacctcgac ctggaacggg tgacctttgc cggccacagc atgggcggcg
     1501 gcatcggcta cctgctgggc ctcgatcacg ctcaccggct ggatcgcctg gtgctcatgg
     1561 cgcccattcc cgccgcgggc atcgccggcc tcgatccgga cgcgcggcag aagcgcctga
     1621 agctgcgccg cgaccagaac cgcgatgccc tgctggcaga gtaccgggcc gcccgctttc
     1681 gcccggacgt ggaaaccgac gactggatcg agagccgcgt ggaccacgtg ctcggcgtca
     1741 gcgacggaca ctacgaggcg agcgcggagt ccatggtcga gctggacgtg ggccaccgac
     1801 tgccagagct ggaggtaccc accctcatgc tcgccggcgc agccgacgag ctgctgcgcg
     1861 caaaccttca cgacttcctc gccctgccca acgccaccct ggaggtgctc tccagagcag
     1921 gccacgaggt ggccgtgcac gagccggacg ccgtggcccg ggccatcgac gccttcatga
     1981 cccacggccc cgtcacggcc cggcacatca ggcagcaaat ggagggggaa tccagatgag
     2041 cgcccctccc acccgtcgca gatggcaggt agccgacgcc ttcggcgacg ggaacatcga
     2101 gatcgccggt ctggactgga gaggcagcgg gccgctggcg ctgctgcatc acgccaacgg
     2161 gttctgcgcc gcaacctgga cgctcgttgc cagaggcctt gccggcagcc atcgggtcat
     2221 cgccatcgat gcccggggcc acggcgactc gggaacacgc cccatccccg aaggatacca
     2281 gtgggacttc ttcgcctcgg atctcaccca ggtggccagg caactgctcg aggaaacagg
     2341 gcagccatcc gtcgagttcg gcatcggcag ctccatgggc ggcatcgtca ccgccgcctc
     2401 ggaagcacgc aacccggggc tcttccagcg catagccatg ctggacccgc cgctacatcc
     2461 gctacccgag ctgcgcgcca ggctcgggct cggcgtcgcg gcgccaaacg cggacctggt
     2521 ggcaatgacg agaaagagaa agacgatctg gccgtcccgc gcgatcgccc ggcaggcgtg
     2581 gcaggacaag ccgcttttcc gcgaatgggt accggccgca ttcgacgcct acctcgaaga
     2641 ggggctcggc gaccgcccgg atgggacggt ggcgctgaag tgcgaccccg gcgtggaagc
     2701 cgctatcttc gaaaccacgg gttctatgga caccttcgag ctcgccccgc gtgtgagcgc
     2761 gcccgtgctg ctcgtccgcg ccctgcaggg ccgggccccc ggagccgcct tcgagcacct
     2821 cgcaggcctt ctgccgcgct gcacctacag ggaggtggac gccggacacc tgctgcccat
     2881 ggaatctccc gagctgacac tggcgctgct tgccgagttc accgagggcg gctgagccgt
     2941 cggcaaccgc ttaggctcgg gcgcgtcagc cagcctcggc cgcgaccagg ccctgatcgg
     3001 ccagcagcgc acgtaccgag gcgtcccgcc cccggaaacg ccggtagacg tcccccgccg
     3061 ggaggcttcc accaagaccg aggacggtgt cccgaaagcg cccggcaatt tctttcatcg
     3121 ccgcgccgtc atcgagcccc gcctccttga aggccgcaaa ggcatccgcc gcaaggacct
     3181 cggcccactt ataggagtag tagccggccg cgtagccacc ggcgaagaga tgaccgaagg
     3241 caggcagcgt actctcgtcg tcgagcctcg gcgtgacgag ggtgctgtcg gcgatgtccc
     3301 gctcgatctc gaaggggctc gcgccgtcgt tgctgcccgc gacgccgaat ctctggtgca
     3361 gggtcatatc gaccttgccg aaaaagagct gccgcagggt ggccgtcccc accatgaagt
     3421 tgcggctgtc gacgatccga tcgagcgtct cgtcgtccag cggctccccg gtatcgaagt
     3481 gggcggtgag atcgcgaaga aagggtttgt gctccatcca gtactcgttg aactggctgg
     3541 cgagctccac ggcgtcccac tcgacgaggt tcatgcccga tgcgccgcca tcctcgatgg
     3601 tcgtgaacat gtgctgggtg gcgtggccga actcgtgaaa gagcgtgcgc acctcgtcca
     3661 gcgacagcaa cgccaccttg ccctctgccg gggggcgcgc gttcatgaca aacagggcca
     3721 cgggcagcga cgacgactta ccaggcgcgg cgagcacccg gctgcgcgac accacggtgt
     3781 tcatccacgc gccgccgcgc ttctcgccag gccgggcgta gggatccacg taaaagccag
     3841 cgatgacctc gccggccccg atcacctcga agaactcgac cgcgggatcc cagacgggca
     3901 ccgcaccggc cgccacgcga cgcacctcga cctcgtatag ccgaccaacc agggaaaaca
     3961 ggccgtcgag aacgcgggga agctgaaaat actcgcgcag gcgctcaccg tcgtaagcgt
     4021 agagcgactc gcgcatcttc tcggaccaga acgccagatc ccagggcgcg ggctgctctc
     4081 cttcgtcgag accgccctca tcgcgcatgt acgccttgag cgtttcgaac tcgcgctccg
     4141 cagcgggccg cgcggctgcc tccagctcgt tgaaaagctg ccagacggcg tccgtgtccg
     4201 gtgccatctt ggcgtccaga ctgcggtcgg catagcactc gaaccccacg agggcggact
     4261 gctcctggcg aagccggagg atttccgtga gcacgcaccg gttgtcgaag tccctggatg
     4321 tgccacgggc gcgaaacgcc cggtagaacc gctcacgcag cccccgatcc agcgcgtgct
     4381 ccatgacggc gacgaagttc acgccgttga cgacgaagtg ccacggaccg gaggcgtccg
     4441 tagcgccgtc ctccccgtct tcccgggcct tggcggctgc cagcccgagg acggccgcgg
     4501 gcactccggc aacctcttca ggcgcctgca ctcggacccg cgacgctttc tcctctttga
     4561 ccaggttcac ggagaaatcg ttgccgagct cggacaggcg ctcctggatc tccttgaagc
     4621 gctccttggg gggcccttcc agatgcacgc ccgaacgttc catggccctg atggactccg
     4681 tgacgattcg ctgccgagcc tggctcagtg ccggccagtc ggggccgtcc cgcaacgcga
     4741 ccagaccccc gtatacgccc aggctctggc tcatccgatt gagcagcgac acgtagatgg
     4801 ggcgaatcgc gtcgtaggcc gcctggagct cgtcggagta cttgacgctg atcaggtggg
     4861 tcaccgcgcc gatcacctga ccgatgcgcg tctgcaggcg ttccaggggc tccatgagcc
     4921 cggcccagga cgcctcgacg tcggtttcca atgcggtgaa agcttcttcg ctctcatcga
     4981 gcagggcgcg aacgccggcc tccacatgag ccgccgtgat gttctcccat acgggcaggt
     5041 gcccgatgga tccgtgctgc cgggtttcct cgatgacggc caggctgcgg gcagccgcgt
     5101 cagatgccga tgtgttcacg atgatctcct tggcaagcct ctcggggccg gcgtgctttg
     5161 agcagcgagg tttgtccaca cgatgaggcg gtttctcccc tcgtccgtcc ggcgggtccc
     5221 ggcgagggga agcgcattct aaggcgtcat tcttcccggg tcagccggcg gatcaggtat
     5281 ccgccggagc gttcgtccat ttccagttcc agcaggcccc ggctttccgc ttcctcgagg
     5341 agatcgctga acgagcggaa gccgtagaaa ctctcgttga accccgggcg ccgccgcttg
     5401 agcgtctgct tcaccatgga gccccagagt ttttcccgct cgccacgctc gccgtacagc
     5461 gcttcagcgg tctccagcac cagcgcgatc ccctcatcgg tgttgccctc ttccgagcgg
     5521 gcgggctcgg ccctcttcgc ctctttggcg tccttgccgt ccatggcgtc cgtggcgtcc
     5581 tttttctcag gcgccttctt tgcggccggc ttcttgcgtg gcttgcgggt cgccttctga
     5641 tggcgaacca gatcgtcgta aaagatgaac tcgtcgcagt tgccgatcag gaggtccgac
     5701 gtcgagttct tcacgccgat cccgatgacg gtcttcgcat tctccctgag cttgctgacc
     5761 agcggcgaga aatcggagtc gccgctgatg atcacgaacg tatcgatgtg ttccttggtg
     5821 tagcagagat ccagggcgtc cacgaccatt ctgatgtccg ccgagttttt acccgactgg
     5881 cgcgtgtggg gaatctcgat cagctcgaac gccgcctcgt gcatgacggg cttgaactcc
     5941 ttgtagcgat cccagtcgca ataggctttc ttcatcacga tgctgccctt gaccagcagg
     6001 cgctcgagca ccagctggat gtcgaacctg gcgtacttcg catcacgcac gccgagggca
     6061 acgttctcga agtcacagaa gagcgcaagg tttcggtctt cgttttcgcg ggccatgagg
     6121 tatgccgcag ctgaggttgc gcctacgtta acggggccgc gtgcggccgt ccacaagcaa
     6181 acggtcacgc aaggcggcga ccaggggatc gcagggctca gatcgacgca agcggaagtg
     6241 acagcgcctc cccgacccga cggggccgcc cccactgggc gtgatcgggt tcacgatttc
     6301 ggggcacaag cgtgcggatc ggcgagggcg ccggtgcctg ctgagacaca ggtaactgaa
     6361 tggcccaacc attgcgggaa ccattacgca ggaccatgtt tggctgatca catgctcgaa
     6421 gatcttttgt ccaagctgcc tcttgagccc gtgccggtca tcgtctgcgc gggcatcgcc
     6481 gttggctgcc tgctgctcgg atggatctgc aaagccatcg gcgccaatcg caagctgggt
     6541 c
//
LOCUS       DBNHQR010000608         1234 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1643448, whole genome shotgun sequence.
ACCESSION   DBNHQR010000608 DBNHQR010000000
VERSION     DBNHQR010000608.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1234)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1643448"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..>1234)
                     /locus_tag="AC16VR_13855"
     CDS             complement(162..>1234)
                     /locus_tag="AC16VR_13855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR6012290.1"
                     /translation="VGLIKLPGLNLFDVPNHVTALRPKHLLSATDFFEWLSMLSGGFP
                     EPYTFGRRLTRYFSRIDDRYDVVHDNQSLCYGLLNVARRTPVICTIHHPITWDRDIAI
                     ANAATRGERLLIRRWHSFLAMQTRVAKRLPNIVTVSERSKRDICAAFEIPLGRVTVVR
                     NGIDTEAFRPDPTVTRKPWQLVTTASADQPLKGAQHLLPAFAQLCRRFPELRLVFIGA
                     PRPGGKTDRLVDALGVRHRMDFIHGISTADIRRLYAESTVAVVPSEYEGFGLPAGEAM
                     ACGTPLVSTDGGALPEVVGQAGRIVPSRDPDALAEAIAGLLVDPKEREALSRAGRARM
                     VEHFAWAEAAERLTALYEQVLA"
BASE COUNT          205 a          386 c          433 g          210 t
ORIGIN      
        1 ccgcgcggtt gtcagatcgc cgagcgacag atccatgccg acgacagtga cggcctccct
       61 gaggtaggcg gtgatggcat ggcggccctc tccgcagcca agatccagga cgttgtcccc
      121 gggggcgagg ttcaggcgcg cgaaatcgac ggtctccaga ctcatgcgag cacctgctcg
      181 tagagagcgg tgagccgctc tgcggcttct gcccaggcaa agtgttcgac catgcgggcc
      241 cggccggcgc ggctgagcgc ttcgcgctcc ttcgggtcga ccagaagccc agcgatggcc
      301 tctgccagcg cgtccgggtc gcggctcggc acgatccggc ccgcctgacc gacaacctct
      361 ggaagcgcgc cgccgtccgt gctcaccagt ggcgtaccgc aggccatcgc ctcgccggcc
      421 ggtagtccga agccctcata ttccgacggg acgacggcca cggtggattc ggcatagagc
      481 ctccggatgt ctgccgtgct gatcccgtga atgaaatcca tgcgatggcg aacgccgagc
      541 gcgtctacca ggcgatccgt tttgcccccg ggacgcgggg cgcctatgaa gactaggcgc
      601 agctcgggaa agcgccggca cagctgggcg aacgcgggta gcaggtgctg ggcgcccttg
      661 agtggctgat cggcactcgc cgtggtgacg agttgccagg gtttgcgcgt cacggtcgga
      721 tccgggcgga acgcctcggt gtcgatgccg ttcctgacga cggtcacccg gcccaggggg
      781 atctcgaaag ccgcgcagat gtcgcgcttg gaccgctcgc tgacggtgac gatgttgggc
      841 agacgctttg ccacgcgggt ctgcatggca agaaagctgt gccagcgtcg gatcagcagc
      901 cgctcgcccc gggttgcggc gttggcgatg gcgatatcgc ggtcccaggt gatgggatgg
      961 tgaatggtgc agatcaccgg cgttcggcgc gcgacgttca gcaggccgta gcacaggctc
     1021 tggttgtcgt gtacgacgtc gtagcgatcg tcgattcggg agaagtatct cgtcaggcgt
     1081 cgcccgaagg tatacggctc cgggaatccg ccgctcagca tcgacaacca ttcgaagaag
     1141 tcggttgccg aaaggagatg cttcgggcgc agcgcggtca cgtgattggg cacgtcgaac
     1201 aggttcaggc ctggcagctt gatcagcccg acgc
//
LOCUS       DBNHQR010000609         2511 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_439383, whole genome shotgun sequence.
ACCESSION   DBNHQR010000609 DBNHQR010000000
VERSION     DBNHQR010000609.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_439383"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2511
                     /locus_tag="AC16VR_13860"
     CDS             <1..>2511
                     /locus_tag="AC16VR_13860"
                     /inference="COORDINATES: protein motif:HMM:NF019410.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HIR6012291.1"
                     /translation="ALEAISALQEPARDDDLTLHEYVPGSARHGEASGDEPDAEEEAA
                     VGVKLATHDEPEAEQVGPAPREVYATARSIVKLERVARQQLEGEGGEPRQAAGGGAAN
                     PFGSEEILRVLGALGPGGQGDRATVCQRLVQELDAADGDRSLGESDLDRFRVLESLID
                     SICEDRFLSQGSLNWVRRLEVTLYKLAVHNPGFPGGDPDRPHPAEIMLNQLARLSGIA
                     DSREAKARELSGRVDALVERVADGYSGDGGIFEEAVAELNPLVDEQIRNYRGNMERIV
                     RASEGQQRLTRARKAVVNALAPKMAGKEVPEPILKLLNPGWRNLMVHTCLRMGAGSAQ
                     WEERVAVIERLWAEFVAEPEQGGRDASDLKQLLRQVADGLISISFDPGLRKPLLLDIA
                     KVLRAKADGKPAPVKTVSVAADGVADALGIARLLPESDAELEAAQQEMQRSWAKSLSR
                     ARTLQAGDWIATDDAQGRPLILVVAFVGDESSGFVLANRNGRKERELTLTEMADNLHE
                     ARMTLLSDFDMPLVERAAERMLETVHGRLADQSGRDALTQLVNRREFERRLDKALKRS
                     RADGVDHALMLIDLEGVEAVRSDTGDAGADELLKGAADVLTRALIGRPADIGRVDADR
                     FGILLERTSGPAAMEIAEQILKALGDDPLRVSSGQQALSASIGLVSMDTQSESANAVM
                     HYAERACRAARLSGRNQIKEFEIGEIAQLRRHGEAAWAFRLDEAMAGEGLIVSFRQIA
                     PMSGARNGVDTHYEVQLTMRDEEGGIMAPEKFQDAAESHERMPALDRWTVAWCLDWMA
                     AHKDSLDHFGGFAIRLSDRSLNEPTFSDFVLERFSACQV"
BASE COUNT          451 a          754 c          922 g          384 t
ORIGIN      
        1 gccctcgaag cgatcagcgc tctccaggag ccggcccgcg acgacgacct gacgcttcac
       61 gaatacgtac cggggtccgc ccggcacggc gaggcttccg gcgacgagcc ggacgccgag
      121 gaggaagccg cggtgggcgt gaagctggct acgcacgatg agccggaagc ggagcaggtt
      181 ggccccgcgc cgcgcgaggt ctacgccact gcccgcagca ttgtgaaact cgagcgcgtc
      241 gccaggcagc agcttgaagg cgaggggggc gaaccgcggc aggcagccgg gggcggcgca
      301 gccaacccgt tcggcagcga ggagatcctc cgggtgctgg gcgcgctggg gccgggaggt
      361 caaggcgacc gggccaccgt ttgtcagcgc ctggtgcagg agctggatgc ggccgacggc
      421 gacagaagcc tcggcgagag tgacctggac cgttttcggg tgctcgaaag cctcatcgac
      481 tccatctgtg aggaccggtt cttgagccaa ggcagcttga actgggtccg gcgcctggaa
      541 gtgactctct acaagctggc ggtacacaac cctggtttcc ctggagggga tcccgaccgg
      601 ccccacccgg ccgagatcat gctcaatcag cttgcccgtc tcagcggcat cgcggattcc
      661 cgggaagcga aggcgcgtga gctgagcggt cgcgtggatg cgctcgtgga gcgtgtcgca
      721 gacggctact ccggggacgg cggcatcttt gaagaggcgg tcgccgagct gaatccgctg
      781 gtggatgagc agatccggaa ctaccgtggg aacatggagc gcatcgtgcg cgccagcgag
      841 ggacagcaac gcctgacccg ggctcgaaaa gcggtcgtga atgccttggc tccgaagatg
      901 gcgggcaaag aggtcccgga gccgatactc aagctgctga atcccgggtg gcgcaacctg
      961 atggtacata cctgcctgcg aatgggtgcg ggaagcgctc agtgggagga gcgggtcgcc
     1021 gtcatcgaac ggctgtgggc cgagttcgtc gccgagcccg agcagggcgg cagggacgcc
     1081 tccgacctca agcagctgct gcggcaggtt gccgacggcc tgatcagcat ctccttcgat
     1141 ccgggtctgc gcaagcctct gcttctcgac atcgcgaagg tgttgcgggc gaaggccgac
     1201 ggcaagccgg cacccgtcaa gaccgtcagc gttgcggcgg acggcgtggc ggacgcgctg
     1261 ggcattgcca ggcttcttcc ggagtcggat gcggaactcg aagccgcgca acaggaaatg
     1321 cagcggagct gggccaagtc gctgagccga gcccggaccc tgcaggcggg ggactggatc
     1381 gccacggatg acgcccaggg acgcccattg attctggtcg tggccttcgt cggcgatgag
     1441 tcgagcggct ttgttctggc caaccgcaac gggaggaagg agcgggaact cacgctgacc
     1501 gagatggcgg acaacctgca cgaagctcgg atgaccctgc tgagcgactt cgacatgccg
     1561 ctcgtggagc gcgcggcgga acggatgctg gagaccgtgc acggcaggct tgccgaccag
     1621 tccggtcggg atgcgctgac gcagctcgtc aaccggcggg agttcgagcg gcggctcgac
     1681 aaggctctga agaggtcgag ggcggatggc gtggatcacg ccctgatgct catcgacttg
     1741 gaaggcgtcg aggcggtgcg gtcggatacg ggagatgcgg gcgccgacga gctgctaaag
     1801 ggcgccgcgg acgttctgac cagggcgctc attggtcggc cggcggacat cggccgcgtc
     1861 gacgcggacc gcttcggcat tcttctcgaa cgcacgagcg ggccggccgc gatggaaatt
     1921 gccgagcaga tcctcaaggc gctcggcgac gatcccttgc gcgtgagcag cggccagcag
     1981 gcgctttccg ccagcatcgg gctggtgagt atggatacgc agtccgagtc ggcaaatgcg
     2041 gtcatgcact atgccgaaag ggcatgccgg gcggccaggc tgtcgggtcg caatcagatc
     2101 aaggagttcg agatcgggga gattgcgcag ctgcggcgcc acggggaagc cgcgtgggcc
     2161 ttcaggctgg atgaagccat ggccggtgag ggccttatcg tgagtttccg ccagatcgcc
     2221 ccgatgagcg gcgcgcgcaa cggcgtcgat acgcactacg aagtgcagct gaccatgcgc
     2281 gacgaggaag gtgggatcat ggcccccgag aaattccagg atgcggcgga aagccacgaa
     2341 cgcatgcccg ccctggaccg ctggactgtg gcctggtgtc tcgactggat ggcggcacac
     2401 aaagacagcc tggatcattt cggcggtttc gcgatccggc tgtcggatcg ctcgctgaac
     2461 gagccgacgt tctcggactt cgttctggag cggttctccg cctgccaggt c
//
LOCUS       DBNHQR010000610         2013 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_187198, whole genome shotgun sequence.
ACCESSION   DBNHQR010000610 DBNHQR010000000
VERSION     DBNHQR010000610.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_187198"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..894
                     /locus_tag="AC16VR_13865"
     CDS             202..894
                     /locus_tag="AC16VR_13865"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007992649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HIR6012292.1"
                     /translation="MGATSALHAAEPEELLKRRLMAEIRGDAKRTADYTGRSALDERV
                     MAALEAVPRHEFVEAWSPGAAWENVPLPIGHGQTISQPFIVALMTDFVEPHPTHRVLE
                     IGTGSGYQAAVLAGLVGEVYTVEIIPALAQTATERLHRLGYDNVFVRVGDGFYGWPEK
                     GPFDGIVVTAVAPEIPAPLLEQLRPGGRMILPVGEVHGAQNLVLVSKDADGAVATRDV
                     LPVMFVPLTGDH"
     gene            complement(906..1715)
                     /locus_tag="AC16VR_13870"
     CDS             complement(906..1715)
                     /locus_tag="AC16VR_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007980046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6012293.1"
                     /translation="MTERYQGYQRLEFDYPHPRILRISFNRPERYNALDVVGHNEITY
                     VWREVDADPDIDCVILTGKGKAFSAGGDFEMIEEIIADFDTRAQTWKEARDLVYNIIN
                     CGKPIISAINGPAVGAGLVAGLLADISIVAESVSLTDGHTRLGVAAGDHAAIIWPLLC
                     GMAKAKYYLLTCDRLSGAEAERIGLVSMCVPDEELESRALSVAVQLANGAPNAIRWTK
                     YALNNWLRLAGPTFDASTALEMLGFTSGEAREGVSSLKEKRDPEFKKGSRI"
BASE COUNT          319 a          639 c          675 g          380 t
ORIGIN      
        1 aacatccctg cggggccggc accgacgatg cagcagcggg tttcgttgct catgccgaca
       61 ggctatgtcg ttgccgacgg gcgcgcaaat ccatacggga caatgcggat gggccggctc
      121 ggccagccat gtcatgctaa atagatgtac tgcgctgcat cgacagggct gcttgcctgg
      181 gcgttcggga gtttcctcct gatgggggcg acgtccgcgc tgcacgccgc agagccggaa
      241 gagctgctca agcgccggct gatggcagag atccgtggtg atgcgaagcg cacggcggac
      301 tataccggcc gaagtgccct cgacgaacgt gtgatggcgg cgctcgaggc cgtgccccgc
      361 cacgagtttg tcgaggcctg gagccccggc gcggcctggg agaacgtccc cttacccatt
      421 ggtcacgggc agaccatttc tcagccgttc atcgtcgcgc tcatgaccga cttcgtcgag
      481 ccccacccga cgcaccgggt gctggagatc ggcacggggt cgggctatca ggcggccgtg
      541 ctcgcggggc tcgtgggcga ggtttacacg gtggagatca ttcccgcgct cgcgcaaacg
      601 gccaccgagc ggctgcaccg gctcggctac gacaacgtct tcgtgcgcgt cggtgacggg
      661 ttttacggtt ggcccgagaa aggtccgttc gacggtatcg tcgtaacggc cgttgcgcca
      721 gagatccctg cgccgctgct ggagcagctg cggcccggcg gtcggatgat ccttcccgtg
      781 ggcgaggtgc atggcgctca gaacctggtg ctcgtcagca aggacgccga cggagcggtg
      841 gcgaccaggg acgtgctgcc ggtaatgttc gttccgctga ccggcgacca ctgacgccac
      901 cgacctcaga tccgggaacc tttcttgaac tccggatccc gcttctcctt gagtgagctg
      961 accccctccc tcgcctcgcc gctcgtgaac ccgagcatct cgagtgccgt ggacgcatcg
     1021 aaggtcgggc cggccagacg cagccagttg ttcagcgcgt acttggtcca gcgaatggcg
     1081 ttgggagcgc cgttggcgag ctgcacggcg accgacaggg cccggctctc gagctcttcg
     1141 tccggcacgc acatggagac caggccgatg cgctcggcct cggcgccgga caaccggtcg
     1201 caggtcagaa ggtagtactt tgccttggcc atcccgcaaa gaagcggcca gatgatggcg
     1261 gcgtggtctc cggccgcgac gccaaggcgc gtgtgaccgt ctgtcaggct gacggactcc
     1321 gccacgatgg agatgtctgc gagaagcccg gcgacgagcc ccgcgcccac cgcgggcccg
     1381 ttgatcgccg atatgatcgg cttgccgcag ttgatgatgt tgtagaccag atcgcgcgct
     1441 tctttccagg tctgcgcgcg ggtgtcgaag tcggcaatga tctcttcgat catctcgaaa
     1501 tcgccgcctg ccgagaaagc cttgcccttg ccggtgagga tcacgcagtc gatgtcgggg
     1561 tcggcgtcca cttcgcgcca gacgtaggtg atctcgttgt gaccgacgac gtccagggcg
     1621 ttgtagcgct ccgggcggtt gaagctgatg cgcagtatcc gtgggtgggg gtagtcgaac
     1681 tccagtcgct ggtatccctg gtagcgttcg gtcatctggt tcccctcgtc ctgctgtcaa
     1741 ttggctcagg ggaacggtac gctttttggg agatctggca agtcgccacg cgtttgcgca
     1801 cggcggcaca atcgaccggg cgttgagctg ccatcatcgg ctgtgccagg tcaatgctca
     1861 aaggagtcag ccatgcgcct gcttttcatc tgcctcgtcg ccctggcccc ggtggctgcc
     1921 gcccatacgc ccgttcccgt gcatggatgt gcatttcccg agcggccgcc ggacgacgtc
     1981 gacgagatga cctgggaccg cttcctggaa ggc
//
LOCUS       DBNHQR010000611         7056 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1452815, whole genome shotgun sequence.
ACCESSION   DBNHQR010000611 DBNHQR010000000
VERSION     DBNHQR010000611.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7056)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7056)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7056
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1452815"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="AC16VR_13875"
     CDS             complement(<1..669)
                     /locus_tag="AC16VR_13875"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6012294.1"
                     /translation="MSDYDVVIVGGGLGGSALGGFLADAGRKVLVLERLTEFSDRVRG
                     EWMACWGVVELKKLGLYERFMAAGGHHLSRSIPYDELLPAERAEAIATKFADLHPDVP
                     GPLCMEHVVMQNEAIAWAAESGADVLRGVSGVAVTAGAKPRVRYTHDGVDHDVGCRWI
                     VGADGRGSSVRRQLDIPINEAPLDHLIAGLLIEGADDWPEDLQCLGKFGDLHYLVFPQ
                     GGGKV"
     gene            complement(680..1630)
                     /locus_tag="AC16VR_13880"
     CDS             complement(680..1630)
                     /locus_tag="AC16VR_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6012295.1"
                     /translation="MAFGHDTTTEEVLQGIDLRGKTALVTGASGGLGAETARALASRG
                     AAVTLAVRNVAKGEDVAESIRESTGSTVDVVELELDSLPSVRACAESWLGNHGPLNLL
                     INNAGVMACPLARTEAGWELQFATNHLGHFLLTLLLLPALRAGAPARIVNLSSGAHRF
                     GGVDFDDIHFERREYDKFVAYGQSKTANILFAVELGRRLEGSGITVNAVHPGVIVTEL
                     GRHLTPEDLANFPGAKSVDELRYKTVESGAATSVWAATSPELEGRSGLYCEDCHVAGP
                     AVEDGVQEGYSSWARDPEAAARLFALSEELVGEKYAGAAA"
     gene            1765..3255
                     /locus_tag="AC16VR_13885"
     CDS             1765..3255
                     /locus_tag="AC16VR_13885"
                     /inference="COORDINATES: protein motif:HMM:NF014165.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012296.1"
                     /translation="MSAYSELEAKAREVARLREVAAITHWDEACMMPRGGGAGRSEAL
                     ASLATLIHERVADPEIGELLNRARGEGFELDDWQSANVTNMEREFVEATAIPSDLVRA
                     SRLAQMKCEQAWREARARNDWETVKPLLAEVIGLARQQADALADAMHLSRYDALLEVY
                     EPELRIADIEPVFEDLKAFLPGALETALDRQGEVAPLEGPFTVEEQRQLSLEVMGLLG
                     FDFDRGRLDVSHHPFCGGVPDDTRITTRYSESSFIEALMATCHETGHALYQQGLPRDW
                     RQQPVGDSLGAAVHESQSLLMELQVCRSREFVGFLAPMIRRHFDRSETEPAWSTDNLY
                     GHVTRVARGYIRVDADEITYPLHVILRFELERQLLDGTLAVADLPEAWNDAMQRYLGL
                     STEGNNADGCMQDVHWYAGLFGYFPTYTLGALTAAQWFATARKVMPDLASDIAAGNLE
                     PLVAWLRENIHGRGRQTTMQPLLEEVTGSTLDAGFYKRHIQARYLG"
     gene            complement(3301..4380)
                     /locus_tag="AC16VR_13890"
     CDS             complement(3301..4380)
                     /locus_tag="AC16VR_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015648740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2855 family protein"
                     /protein_id="HIR6012297.1"
                     /translation="MHGLQLLVNRNDLEDTRWQQAPVPAPDELAPGEVLLRIDEFALT
                     ANNITYAVAGEMMRYWNFFPAPDGWGRVPVWGFATVVASNHAEVATGERLYGYLPMGS
                     HLLIEAGRVRANQLTDVSAHRVDLPPVYNQFQRVDAASEPAADAAQMLYRPLFMTSFV
                     LDHFLHANGFFGAQSVILTSASSKTALGLALLLARRGDDRVIGLTGRANVGFVEGLGC
                     YDEVVAYEDLATLDAAREVVSVDMAGNGEVLAALHNHFADNLKYSCLVGATHWDHRAG
                     AREMAGPEPELFFAPSHIQDTDWGPGGLEARFADAWDMLVERMSGLVTVVRADDPAEV
                     EAVYQRLLAGEVGPDVGYVLGVDVG"
     gene            complement(4396..5265)
                     /locus_tag="AC16VR_13895"
     CDS             complement(4396..5265)
                     /locus_tag="AC16VR_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012492794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6012298.1"
                     /translation="MELDGFETREIELDGVRMHVRIGGAGPPLLLLHGYPQTHLMWHR
                     IAPRLARNFFVVAPDLRGYGASGKPPAGEEYRAYSKRVMAAEMVALMAGLGHERFAVA
                     GHDRGGRVAYRMALDHPEVVSRLVILDIIPTLDQFEALGWRGGLFGYHWYFLAQPPPL
                     PETLIAHEPELYLRTAMSSWAGDFSALDAAFPAYLAAFTPETIRASCDDYRAGAYVDT
                     EIDRADREAGRRIGCPVLALWGEINGPRPGVLEAWQRWADDVQGVGLPCGHFLPEEAP
                     EAVLAAMLEFLKA"
     gene            5565..>7056
                     /locus_tag="AC16VR_13900"
     CDS             5565..>7056
                     /locus_tag="AC16VR_13900"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6012299.1"
                     /translation="MPKQMHITFILLAISSTFAENVRAERTTTTQIEEIVVTAQKREE
                     SMQDVAISMSAMDANAIEKSFSRTIDEITGMSPNLVINPILGNGTVGVSIRGMQHAEV
                     EKSFDPAVAIYQDGIYLSSTNGALLNVRDAERVEVLRGPQGTLFGRNTIGGLVHVIRS
                     KPTGEIGGKIVATIAEDSQEDFKALLNLPEIGGVSLKLSAASSKGGAYIKNASRGTTD
                     GENDLFMFTIDALWQPTDNFDLRVIYDNIDDDTPTRPVTALTPSGEAFGAFCGDNCAV
                     GKESKALYTTYTTQEQFAELHTDAWTIHANWQFHENHKLAFVFGDRDTDELARQEFDG
                     VAADLFWTDRPTFESQTSYELRLESDWNERLRSTLGVFLWESDYTLQQNTGLFNIFNP
                     TSVTNFTASPFYQQDVDSMGVFGQVDWDITDRLTLSLGGRWLDEEKSACMTVTGYSEN
                     PFQQILDLPPGEVVYVDVAGYEKTLDPYPLFPGPGDLTAGSWGEDCP"
BASE COUNT         1321 a         2415 c         2184 g         1136 t
ORIGIN      
        1 caccttgccg ccgccttgtg gaaataccag gtagtgcagg tctccgaact tgccgaggca
       61 ctgcagatct tccggccagt cgtcggcgcc ctcgatcagg agcccggcta tcaggtgatc
      121 cagaggcgct tcgttgatgg gaatgtcgag ctggcgccga acgctggacc cgcggccatc
      181 ggcgccgacg atccagcgac agccgacgtc atggtcgacg ccgtcgtgcg tgtaacgcac
      241 gcgcggcttg gcgcccgccg tcacggcaac gccgctcacc ccgcgcagaa cgtccgcacc
      301 ggactccgcc gcccaggcga tggcctcgtt ctgcatcacc acgtgctcca tgcacagcgg
      361 tcccggcaca tcggggtgga ggtcggcaaa tttggtggcg atggcttccg cccgttccgc
      421 gggaagcagc tcgtcgtagg gaatgctgcg ggataagtga tgaccgcccg cggccatgaa
      481 gcgctcgtag agtccgagct tcttcagctc caccaccccc cagcaggcca tccactctcc
      541 gcgcacccgg tccgaaaact ccgtcagccg ctcgagcacc agcactttgc gcccggcgtc
      601 agccaggaag cccccgagcg cgctgccgcc cagcccgccg cccacgatca ccacgtcgta
      661 gtcactcatc ggcagaccct caagctgccg cgccggcgta cttctcgccg acgagctcct
      721 cggacagcgc aaacagccgt gccgccgcct ccgggtccct ggcccaggac gagtaaccct
      781 cctgaacgcc gtcctccacc gcgggacccg cgacgtgaca gtcctcgcag taaaggccgc
      841 tgcgcccctc cagctccggc gaggtagctg cccacacgct ggtggcggca ccggactcga
      901 cggtcttgta cctcaactcg tccacggatt tcgcacccgg aaagttggcc aggtcttccg
      961 gcgtgagatg ccggccgagc tcggtcacga tgacgcccgg gtgcacggcg ttgacggtga
     1021 tcccgctgcc ttccaggcgc cggccgagct ccaccgcaaa gagtatgttg gccgtcttgg
     1081 actgaccgta cgcgacgaac ttgtcgtact cgcgccgctc gaagtggatg tcgtcgaaat
     1141 ccacaccgcc gaagcggtga gcgccggagc tcaagttgac gatccgcgcc ggtgcgccgg
     1201 cccgaagcgc cggcagcagg agcagcgtca acaggaagtg gccgagatgg ttggtcgcga
     1261 actgaagctc ccaccccgcc tccgttcggg ccagcgggca ggccatgacg ccggcgttgt
     1321 tgatgagcag gttgagcggg ccgtgattcc caagccagct ttcggcacag gcgcgcacgc
     1381 tgggaagcga atccagctcc agctcaacca catccaccgt gctgcccgtc gactcccgga
     1441 tgctctccgc cacgtcttcg cccttcgcca cgttgcgcac cgcgagggtg accgccgcgc
     1501 ccctggatgc cagcgcccgg gccgtctcgg cgcccagccc cccggaggcc ccggtgacca
     1561 gcgccgtctt gcccctgagg tcgatgccct gcagaacctc ctcagtggtg gtgtcgtgtc
     1621 caaaagccat gccgctgatc ccccaaaaaa tacccgctca atcctatcca tccggccatc
     1681 ggggcgccac ggcccaacgc tcccaaaacg acgccgaagg tctttatact gcgccgctcc
     1741 aaattcaatc ccgccaggaa gaccatgtcc gcctactcgg agctcgaagc caaagcccgc
     1801 gaagttgccc gcctgcgcga ggtggcagcc attacccact gggacgaggc ctgcatgatg
     1861 cccaggggcg gcggcgcggg ccgctccgaa gcgctggcgt ccctggccac actgatccat
     1921 gagcgcgtgg cggatccgga aatcggcgag ctgttgaatc gcgcgcgtgg ggaggggttc
     1981 gagctcgatg actggcaatc ggccaacgtg accaacatgg agcgggagtt cgtggaagcc
     2041 acggcaatcc cttcggacct ggtgcgggcg agccggctgg cgcagatgaa atgcgagcag
     2101 gcctggcgcg aggcccgggc gcggaacgac tgggagacgg tgaagcccct cctcgcggag
     2161 gtgatcggtc tcgcccgtca gcaggcggat gccctggccg atgccatgca tctgtcccgc
     2221 tacgatgcgc ttttagaggt ctacgagccg gagctgcgca tcgccgacat cgagcccgtc
     2281 ttcgaggatc tcaaggcgtt tctccccggc gcgctggaga cggccctgga tcgccagggc
     2341 gaggtggcgc ccctcgaggg gcctttcacg gtggaggagc agcgtcagct gtccttggag
     2401 gtcatgggcc tgctcggctt cgacttcgat cggggcaggc tagacgtcag ccatcacccg
     2461 ttctgcggcg gcgtgccgga cgatacccgc atcaccaccc gttactctga gtcgagcttc
     2521 atcgaggcgc tcatggcgac ctgccacgaa accgggcacg cgctctacca gcagggtctg
     2581 cccagggact ggcggcagca gccggtcggc gactcactgg gtgcggccgt ccacgaaagc
     2641 cagtcgctgc tcatggagct gcaggtctgc cgcagccgcg agttcgtcgg ctttctggcg
     2701 cccatgatcc gccggcactt cgaccgcagc gagaccgagc cggcgtggtc gacggacaac
     2761 ctgtacggcc acgtcacccg cgtggcgcgc ggctacatcc gcgtggacgc tgacgagatc
     2821 acctacccgc tccacgtcat cctgcgtttc gagctggagc gccagctgct ggacggcacc
     2881 ctcgccgtgg ccgacctgcc cgaggcatgg aacgacgcca tgcagcgcta tctggggctg
     2941 tccaccgagg gcaacaacgc agacggttgc atgcaggacg tgcactggta cgcgggcctt
     3001 ttcggttact tcccgaccta taccctgggc gcgctcaccg ccgcgcaatg gttcgccacc
     3061 gcccgaaagg tcatgccgga tctcgcctcg gacatcgccg ccggaaacct cgagccactt
     3121 gtggcctggc tgcgggagaa catccacggc cgcggtcggc agacgaccat gcagccgctc
     3181 ctggaggaag tcaccggctc gacgctggac gccgggttct acaaacggca cattcaggcg
     3241 cgttatctcg gctgaggccg cctcgccacg atcggatcgc cccaaaaggg gcggcgcgat
     3301 tcaacccacg tcgactccca gcacgtagcc cacgtctggt cccacctctc cagcaagcag
     3361 ccgctggtag acggcctcga cctctgccgg gtcatcggcc cgaaccacgg tgacgagccc
     3421 cgacatacgc tccacaagca tgtcccaggc atcggcgaag cgggcttcga gcccgccggg
     3481 gccccagtcc gtgtcctgaa tgtggctcgg cgcgaagaac agctccggtt cggggcccgc
     3541 catctccctc gcgccagcgc ggtgatccca gtgggtggcc cccaccaggc agctgtactt
     3601 caagttgtcc gcgaagtggt tgtgcagggc ggccagcacc tcaccgttac cggccatgtc
     3661 gacgctcacc acttcccgtg cagcatccag agtggccaag tcttcatagg ccactacctc
     3721 gtcgtagcag ccgagccctt ccacgaaccc gacgttggcc cggccggtga gaccgatcac
     3781 gcgatcgtcc ccgcgccgcg caaggagcag cgcaagcccg agagccgtct tgctggacgc
     3841 gctggtcaga atcaccgact gcgcgccgaa gaagccgttt gcatggagaa aatggtccag
     3901 cacgaaagag gtcatgaaca acggccgata gagcatctgg gccgcgtcag cggccggctc
     3961 ggaagcagca tcgacgcgct ggaactggtt gtagaccggg ggcaggtcga cgcgatgggc
     4021 gctgacgtcg gtaagctggt tggcccgcac ccggccggct tcgatgagca gatggctgcc
     4081 catgggcagg tagccgtaga ggcgctcgcc cgtagccacc tcagcgtgat tcgacgcgac
     4141 cacggttgca aatccccaga ccggcacgcg gccccagccg tcgggcgctg gaaagaagtt
     4201 ccagtagcgc atcatctccc cagccaccgc ataggtaatg ttgttggcgg tgagggcaaa
     4261 ctcgtcgatg cgcagcagca cctcgcccgg ggccagctcg tcgggagccg ggaccggcgc
     4321 ctgctgccag cgggtgtcct cgagatcgtt ccgattaacc agcagctgaa gaccgtgcat
     4381 tcatcctctc cctattcagg ctttgagaaa ttctagcatc gcggccagaa ccgcctcggg
     4441 tgcctcctcg ggcaggaagt ggccgcaggg cagcccgacg ccttggacgt cgtccgccca
     4501 gcgctgccag gcctcgagca ctccaggccg cggcccgttg atctcgcccc acagtgccag
     4561 caccgggcag ccgatccgac ggccagcctc acgatcagcc cgatcgattt cggtgtcgac
     4621 gtaggctccg gcccggtaat cgtcgcagct ggcgcggatc gtctccgggg tgaaggccgc
     4681 gaggtacgca ggaaaggccg catcgagggc agagaagtcg ccagcccagc tgctcatggc
     4741 cgtgcgcagg tagagctccg gctcgtgggc gatgagggtc tccggcaggg gcggtggctg
     4801 agcgaggaag taccagtggt agccgaaaag accgccgcgc cacccgaggg cctcgaactg
     4861 atccagggtc ggaatgatgt ccaggatgac aagccggctc accacttccg gatgatccag
     4921 cgccatgcgg taggccacgc gaccgccgcg gtcgtgcccg gccacggcga agcgctcgtg
     4981 accaaggccc gccatcagcg ccaccatctc cgccgccatc acccgcttgg aataggcgcg
     5041 gtattcttcc ccggccggtg gcttgccgct ggcaccgtag ccgcgcaggt cgggcgccac
     5101 cacgaaaaaa ttccgcgcga gtcggggggc aatccggtgc cacatgagat gagtctgagg
     5161 gtagccgtgc agcagcagga ggggcggtcc agcgcccccg atccgaacgt gcatgcgcac
     5221 gccatccagt tcgatctcgc gggtctcgaa gccgtccagc tccattgtta tcccccggcg
     5281 ttctgcctcg acggccccga cctactcccg ggcgagccgg gtccaagtgc cccgatcgtt
     5341 atccaatacc cggcccttca ggtcgttaac ttcgtacccg taacgtgccc gctcggtgca
     5401 gggaggggac atttcagacc ggaatcacat caacccgcac caacacgctc caggcgtccg
     5461 agctctcgag gcgactggac tccgtttgta catcaaagta gagtccccgc ccgaatcgct
     5521 tccacagcag aagcggccac gagataagac tatcgagggg aaacatgcca aagcaaatgc
     5581 acataacgtt catcctcctg gcgatctcgt caacgttcgc cgaaaacgtt cgtgccgaga
     5641 gaacgaccac cacgcagatc gaagagatcg tcgtgactgc gcagaagcgc gaggaaagca
     5701 tgcaggacgt tgccatctca atgtccgcca tggatgccaa tgcgatcgag aaatcattct
     5761 cccgaacgat cgacgagatt accgggatgt cgcccaacct ggtgatcaac ccaatcctgg
     5821 ggaacggaac ggttggcgtg tcgattcgcg ggatgcagca cgctgaggtt gaaaagagtt
     5881 tcgatccggc cgtcgccatc tatcaggacg gtatctatct gtcatcgacc aacggcgcct
     5941 tgctgaacgt cagggacgcc gagagagtcg aagtgctcag ggggccccag ggcaccctgt
     6001 tcggcagaaa caccatcggc ggcctggtcc atgtcatccg ctccaaaccg acgggagaga
     6061 tcggcgggaa gatcgtggcc accattgccg aggacagcca ggaagacttc aaagcgctgc
     6121 tcaatctgcc ggaaatcggt ggcgtgtcac tgaagctgtc ggccgccagc agcaagggcg
     6181 gcgcctacat caagaacgcg tcccgcggca ccaccgatgg ggagaacgac ctcttcatgt
     6241 tcaccatcga cgcgctttgg cagcccaccg acaatttcga tctgcgagtc atctacgaca
     6301 acattgacga cgacacgcca acgcgtccgg tcaccgcatt gaccccatcg ggcgaagcct
     6361 tcggagcctt ctgcggcgac aactgtgccg tgggcaagga atccaaggct ctttatacca
     6421 cctacacgac gcaggagcag ttcgccgagc tgcacaccga cgcgtggacc attcacgcaa
     6481 actggcagtt ccacgagaat cacaagcttg ccttcgtctt cggcgatcgc gacacggatg
     6541 aacttgcgcg ccaggagttc gacggcgttg cggcagacct attctggacg gaccgcccga
     6601 cgttcgaaag ccagaccagc tacgagctgc gactcgaatc ggactggaac gaacgcctgc
     6661 gatccactct cggcgtcttt ttgtgggaga gcgattacac gctgcagcag aacaccggcc
     6721 tcttcaacat cttcaatccc acgagcgtta caaacttcac cgcctccccc ttctaccaac
     6781 aggacgtgga ctcgatggga gtcttcggac aggtcgattg ggacattacc gatcgtctga
     6841 cgctcagcct cggcggaagg tggctcgacg aggagaagtc ggcctgcatg acggtgacgg
     6901 gctattccga aaacccgttc caacagatcc tggatctgcc cccgggtgag gtggtttacg
     6961 tcgacgtcgc gggctacgaa aagacgctcg atccctaccc gctgtttcca ggcccgggag
     7021 acttgacagc cggatcgtgg ggcgaagact gtcccg
//
LOCUS       DBNHQR010000612         2215 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1136852, whole genome shotgun sequence.
ACCESSION   DBNHQR010000612 DBNHQR010000000
VERSION     DBNHQR010000612.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1136852"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..988)
                     /locus_tag="AC16VR_13905"
     CDS             complement(137..988)
                     /locus_tag="AC16VR_13905"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020039.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR6012300.1"
                     /translation="MNVELELLASGYRLLEGPRTDADGALWFSDVYGGTVNRRDPDGI
                     IEVVVRDRRMVGGLLMHANGGFVMTGPSVVLWRDGQERVLLERDGVRFFNDLHADASG
                     RIYVGSIRADIANLDDEPDPPGEAYRIDLDGSVTELYGGVGISNGIGFSPDGRTLYHV
                     DSTSRGIWAHDLDDAGALSNRRHIAKPAFEKGIPDGLCVDTEGNLWVAHVQGGRVVVL
                     DPGGNHVDEIRVPASWVTSCAFGGSDYRDLYIVSADNTDEKALGGCVWRCRPGAAGHP
                     TPLARVG"
     gene            complement(1006..1506)
                     /locus_tag="AC16VR_13910"
     CDS             complement(1006..1506)
                     /locus_tag="AC16VR_13910"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6012301.1"
                     /translation="MAYTPEQLSDLECIRDAAKRYCRGVDRLDEALMKSAYWPEATDD
                     HGVFVGNAMEFAEMCMVSHLRWRSTSHCIFNHSIELDPDGIHARGEAYNVTFLFQDGA
                     DVLDTWYGRYLDVYEKRGDEWRILERVCVHEGTTTGPVSKMEIDAEKFRHGSFDRPSS
                     GRRIGP"
     gene            1636..>2215
                     /locus_tag="AC16VR_13915"
     CDS             1636..>2215
                     /locus_tag="AC16VR_13915"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR6012302.1"
                     /translation="MVDDSGTFPTAGVLGRAFRSGELSPVAVTRELLERIDRLNPALG
                     AVHRLCPERAMAEAAAAERAFSAGEARSPLQGVPYLVKDLFDVAGLPTTAGTRLLEDN
                     VAEADSTAVARLAAAGMVLLGKTVTVQFAYGGAGVNHDHGTPRNPWRQDHYLPGGSSS
                     GSAVAVAAGLAPLALGSDTGGSVRIPASLCGIT"
BASE COUNT          340 a          737 c          751 g          387 t
ORIGIN      
        1 ggcggcgcgg cgaacaccac cgccggcatt gagctggtgt cgaagctgtc gggcctcacg
       61 gccatgaacc tgctcggcaa ggactgccac gagcccctga cccggatgct gcgcgacaag
      121 ctcggtcttt agccgttcag ccgacgcgcg ccagcggggt gggatgcccc gcggcgccgg
      181 gccggcagcg ccagacgcag ccgcccaggg ctttttcgtc cgtgttgtcg gcgctcacga
      241 tgtacaggtc ccgatagtcc gagccgccga aggcgcagct ggtgacccag ctggcgggca
      301 cgcggatctc gtcgacatgg ttgcccccgg ggtccagaac gaccacccgg ccgccctgaa
      361 cgtgggcgac ccagagattt ccctcggtgt ccacgcacaa gccgtcgggg atgccctttt
      421 cgaaggcggg cttggcgata tgtcgtctgt tggacaacgc gcccgcgtcg tccagatcgt
      481 gcgcccagat tccccggctg gtcgagtcga cgtggtacag agtccgtccg tcaggagaga
      541 aaccgatgcc gttggagatg cccacgccgc catagagctc cgtgacgctg ccgtccaggt
      601 cgatgcggta ggcctccccc ggcgggtccg gctcgtcgtc caggttggcg atgtctgcac
      661 gtatcgagcc aacgtagatg cgccccgagg cgtcggcgtg cagatcgttg aagaagcgca
      721 cgccgtcgcg ctcgagcagc acgcgctcct gaccgtcgcg ccagaggacg acgctgggtc
      781 cggtcatcac gaaaccgcca ttcgcgtgca tgagcaggcc gccgaccatt cgccggtcgc
      841 gcacgacgac ctcgatgatg ccatccggat cgcgccggtt caccgtcccg ccgtagacgt
      901 ccgaaaacca cagcgccccg tctgcgtcgg tgcgcggacc ctctagaaga cgatagccgc
      961 ttgccaggag ttccagctcg acgttcatag gcttgtttcc cgtgctcagg ggccgatgcg
     1021 gcgcccggat gatggtcggt cgaaggatcc gtgccgaaac ttttcggcgt cgatctccat
     1081 cttgctcacc ggcccggtgg tggtgccctc gtggacgcag acgcgctcta agatgcgcca
     1141 ctcgtcgccg cgcttctcgt agacgtccag gtatcggccg taccacgtgt cgagcacgtc
     1201 ggcgccatcc tggaacagaa aggtgacgtt gtatgcctcg cctcgcgcgt gaatgccgtc
     1261 ggggtcgagc tcgatgctgt ggttgaaaat gcagtggctg gtggagcgcc agcgcaggtg
     1321 gctcaccatg cacatctctg cgaactccat cgcgttgccg acgaacacgc cgtgatcgtc
     1381 cgtggcctcc ggccagtagg ccgacttcat caacgcctcg tccaatcggt cgacgccgcg
     1441 gcagtagcgc ttcgcggcgt cccggatgca ctcgagatcc gacagttgct caggcgtgta
     1501 agccatgtgt ctctcccctg atcagctaag cggctcaatt attcaacgtc gcgacgccgg
     1561 gagccagcca catctggggg ccccgccgag gtggctgcta gaatgccgcc ttctcccggg
     1621 ggccggaggt cgagcatggt agatgactct ggaacgttcc cgacggccgg tgtgctgggg
     1681 cgggcatttc gcagcggcga gctctcgccg gttgcggtga cccgtgagct gctcgagcgg
     1741 atcgatcgac tgaacccggc tctgggcgcc gtgcatcggc tgtgccccga acgggccatg
     1801 gcggaagccg cggcggcgga gcgggctttt tcggccggcg aggcgcggag tcccctgcag
     1861 ggtgttccct acctggtcaa agatcttttc gacgttgccg gtctgcccac caccgcaggg
     1921 acccgtctgc tggaggacaa cgtggcggag gcagattcca ctgccgtggc caggcttgcc
     1981 gctgcgggca tggtgctcct gggcaagacg gtgaccgtgc agttcgccta cggcggggcc
     2041 ggtgtgaacc acgatcacgg cacgccgcgt aacccttggc gtcaggacca ctaccttccg
     2101 gggggatcca gcagcggctc ggcggtggcg gtggcggcgg gtctcgcgcc gttggcgctc
     2161 ggcagcgata cggggggatc cgtccggatc ccggcctccc tctgcgggat cacgg
//
LOCUS       DBNHQR010000613         3266 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1453188, whole genome shotgun sequence.
ACCESSION   DBNHQR010000613 DBNHQR010000000
VERSION     DBNHQR010000613.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1453188"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="AC16VR_13920"
     CDS             <1..543
                     /locus_tag="AC16VR_13920"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HIR6012303.1"
                     /translation="IRYPMVEVLTGAATITVLALFGYTWLGLAAAVYTWAMIALTFID
                     YDTQLLPDQITLPLLWLGLITCHFGGVTDLGSGVLGAVIGYLFLWTTFWAFKLITGKE
                     GMGYGDFKLFAAIGAWLGWQVLPGTILIAATLGLAYALAITLAGKREAAQPIPFGPFL
                     AVAGWVSLMARDNVLTLFAA"
     gene            573..1172
                     /gene="coaE"
                     /locus_tag="AC16VR_13925"
     CDS             573..1172
                     /gene="coaE"
                     /locus_tag="AC16VR_13925"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003302803.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HIR6012304.1"
                     /translation="MSRLVIGITGGIGSGKTAVSDRFKALGVTVVDADIASRVIVEPG
                     RPALVAIAEHFGCDVIQADGSLDRAELRRRVFADPDERRWLEQLTHPLINEHIREELE
                     QAESPYAVLVSPLLAETGQSRFCHRILVVDVPEEVQVRRTMARDANDEAQVRKIIEAQ
                     ASREQRLALADDVIVNDSSLEVLDEEVARLHQVYTEMAQ"
     gene            1169..1381
                     /gene="yacG"
                     /locus_tag="AC16VR_13930"
     CDS             1169..1381
                     /gene="yacG"
                     /locus_tag="AC16VR_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014821358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HIR6012305.1"
                     /translation="MTASEPDRTARIVTCPTCGGRVKWTSGNEFRPFCSERCKLMDLG
                     AWASEQHRIPGNEVDDDLLSGDLDPD"
     gene            complement(1409..2629)
                     /gene="argJ"
                     /locus_tag="AC16VR_13935"
     CDS             complement(1409..2629)
                     /gene="argJ"
                     /locus_tag="AC16VR_13935"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019471521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - L-glutamate N-acetyltransferase
                     activity, acting on acetyl-L-ornithine as donor [Evidence
                     IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HIR6012306.1"
                     /translation="MAVNVPPLQELLVVPGVSLGTAGAAIKRQGRDDIAVLLLHPQTR
                     AAGVFTRSSFRAAPVTLADRRLSGGRARALVINSGNANAATGDPGLADAVAVCDAVAA
                     ALDVPGDSVLPFSTGVIGERLPVSRMLPAVVSACGSLAPDGWEAAARAIMTTDTAPKA
                     VSRRVRVAGEEVVITGMAKGAGMIKPDMATMLGFVCCDAAVADGCLPAMVREVAGRSF
                     NRITVDGDTSTNDAFVVCATGCAENAPVTDVGSEGYAELRDGLAAVATELAQSIVRDG
                     EGATRFVTVRVSGGESAAECLKVAYTVAESPLVKTAIFAGDPNWGRFCMAIGRAGIAD
                     LDTGRVAVHLDDVPIALGGLVAPQYEEAAAARVMARQEFEVHVDLGRGSAEETVWTTD
                     LSYEYVRINAEYRT"
     gene            complement(2643..>3266)
                     /locus_tag="AC16VR_13940"
     CDS             complement(2643..>3266)
                     /locus_tag="AC16VR_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008937205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HIR6012307.1"
                     /translation="GLEQALQTEFASRQPVRGWLDEDDALNEDSLSRKILAEVEAEYR
                     AKEQDWASHGIDMRVVEKQIMLQVLDQRWKEHLAAMDYLRQSIHLRAYAQKQPKQEYK
                     RESFQLFQELLENIKRDVIRLLCRVQIETSDELEEAERRRREEAERRMRYTHAESSGL
                     DGGEQEQPDDGAGKPETFVRSERKVGRNEPCPCGSGKKYKQCHGKAA"
BASE COUNT          541 a         1136 c         1030 g          559 t
ORIGIN      
        1 atccgctacc ccatggtgga ggtgctcacc ggggccgcga ccataacggt gctggcgctc
       61 ttcgggtaca cgtggctggg tctagccgcc gctgtgtaca cctgggcaat gatcgcgctg
      121 acgttcatcg actacgacac ccagctgctt cccgaccaga tcacgctgcc gctgctctgg
      181 ctgggcctga tcacctgcca cttcggcggc gtcacggatc tgggctccgg ggtcctcggc
      241 gccgtgatcg gctacctgtt tctctggacg accttctggg ctttcaagct catcaccggc
      301 aaggagggca tgggctatgg cgacttcaag ctcttcgcgg ccatcggagc gtggctcgga
      361 tggcaggtgc tgccgggcac gatcctcatc gccgcgacgc tggggctcgc gtatgccctg
      421 gcgatcacgc tggccggcaa acgggaggca gcgcagccga tcccatttgg gcccttcctg
      481 gcggttgcgg gctgggtttc gcttatggcc cgtgataacg tgctgaccct gtttgcagcc
      541 tgaccggtcc tccgacctca aacgacatcc caatgagcag actggtgatc ggaatcaccg
      601 gcggcatcgg cagcggcaag accgccgtct ccgaccggtt caaagcgctg ggggtaaccg
      661 tcgtcgacgc cgacatcgcc tcccgcgtga tagtcgagcc aggccgacca gccctcgtcg
      721 ccatcgccga gcactttggc tgcgatgtca tacaggcgga cggatcactc gatcgggcgg
      781 agcttcgacg cagggtgttc gcagaccccg acgagcgccg ctggctggag cagctcaccc
      841 acccgctgat caacgagcac atccgcgagg agcttgaaca ggccgagtct ccctacgccg
      901 tgctcgtcag cccgctgctg gccgagaccg gccagtcgcg attctgtcat cgcattctgg
      961 tggtggacgt gccggaggaa gtacaggtgc ggcgaaccat ggcccgggac gccaacgacg
     1021 aggcccaggt gagaaagatc atcgaggccc aggcatcccg cgagcagcgc ctggccctcg
     1081 ccgacgacgt catcgtcaac gactcgagcc tggaggtgct ggacgaggag gtggcgcgcc
     1141 tacaccaggt ctacacggag atggcgcaat gaccgcctcc gaacccgacc ggaccgcgcg
     1201 catcgtcacc tgcccgacct gcgggggccg cgtcaaatgg acgtccggca acgagttccg
     1261 acccttctgc tccgagcggt gcaagctcat ggacctcggc gcctgggcca gcgagcagca
     1321 cagaattccg ggcaacgagg tggacgacga tctgctctcg ggggatctgg acccggactg
     1381 accggccagg ccgagaagct tccatggatc aggtgcgata ctcagcgttg atgcgcacgt
     1441 actcgtagga cagatcggtt gtccagaccg tctcctcggc gctgccgcgg ccgaggtcga
     1501 cgtggacttc gaactcctgc cttgccatca ccctggcagc ggcggcttcc tcgtactgcg
     1561 gcgccacgag cccgcccagg gcgatgggca cgtcgtccag gtgaacggcg acgcgaccgg
     1621 tgtcgagatc ggcaatgccc gccctgccga tggccatgca gaaccgcccc cagttcggat
     1681 cgccggcaaa tatcgccgtt ttgaccagag gggactcggc taccgtgtag gccaccttga
     1741 gacattcggc cgcactctcg ccgccggaga cgcgcacggt gacgaatcgc gtggctccct
     1801 cgccatcgcg aacgatgctc tgagccagct cggtagccac cgcggccagg ccatcgcgaa
     1861 gctccgcgta gccttcggag ccgacgtcgg tcacgggcgc gttctccgcg catcccgtcg
     1921 cgcagaccac gaaggcgtcg ttggtggacg tgtcgccatc cacggtgatt cgattgaacg
     1981 agcggccggc cacttccctc accatcgcgg gaaggcagcc gtcggcgacg gcagcgtcgc
     2041 agcagacgaa gcccagcatc gtggccatgt cgggcttgat catgcccgct ccctttgcca
     2101 tgccggtgat caccacctct tcgcccgcga ccctgacgcg acggctgacg gccttgggcg
     2161 ccgtgtccgt ggtcatgatg gcgcgagcgg cggcctccca gccgtccggc gccaggctcc
     2221 cacaggcgga aacgaccgcc ggcagcattc tggaaacggg gagacgctca ccgatgaccc
     2281 cggtggagaa cggcagcacg gaatcaccgg gcacatccag ggcggcggca accgcatcgc
     2341 agacggcaac ggcatcggca agaccgggat ccccggtggc cgcgttggcg ttaccgctgt
     2401 tgatcaccag agctcgcgcg cgcccgccgg aaagtcgccg atcggccagg gtcacgggag
     2461 cggctcgaaa cgagctgcgc gtgaacacgc ccgccgccct ggtctggggg tgcagcagca
     2521 ggacggcgat gtcgtcacgg ccctgtctct tgatggcagc ccctgcagta cccaaagaca
     2581 caccggggac caccagcagt tcctgcagcg gaggaacgtt caccgccatg tcgggcttcc
     2641 ccctacgcag ccttgccgtg acactgtttg tacttcttgc ccgagccgca cgggcaaggc
     2701 tcgttgcggc ccaccttccg ctcactcctc acgaaggtct cgggtttgcc ggcgccatcg
     2761 tccggctgct cctgctcacc cccgtcgagc ccgctggact cggcatgggt gtaacgcatg
     2821 cgacgttcgg cctcttcgcg ccgacggcgc tcggcttcct ccaactcgtc ggaggtctcg
     2881 atctgcaccc ggcagagcag gcggatcacg tcccgcttga tgttctccag cagttcctgg
     2941 aacagctgaa agctctcccg cttgtattcc tgcttgggct gcttctgggc gtaggcacgc
     3001 agatgaatgc tctggcgcag gtagtccatg gccgccagat gctccttcca gcgctggtcc
     3061 agaacctgca gcatgatctg cttctcgacc accctcatat cgatcccgtg ggacgcccaa
     3121 tcctgctcct tggcgcgata ctccgcctcg acctcagcca ggatcttgcg cgacaacgaa
     3181 tcctcgttga gcgcgtcgtc ctcgtcgagc cagcctctca ccggctgcct gctggcaaac
     3241 tcggtctgca gggcctgctc caggcc
//
LOCUS       DBNHQR010000614         9837 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_188019, whole genome shotgun sequence.
ACCESSION   DBNHQR010000614 DBNHQR010000000
VERSION     DBNHQR010000614.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9837)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9837)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9837
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_188019"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..177
                     /locus_tag="AC16VR_13945"
     CDS             28..177
                     /locus_tag="AC16VR_13945"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6012308.1"
                     /translation="MLSGQFLMEMLGERVTLKAGDILHVPAGVVHCASVLGEEPVVSL
                     DATKL"
     gene            276..578
                     /locus_tag="AC16VR_13950"
     CDS             276..578
                     /locus_tag="AC16VR_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012309.1"
                     /translation="MEIELKNGRVIHARPDATGSCAILTGPAPSEPRRWRDLTTDLPF
                     AFGRDAVISLAEPIEGEQTIVGLRVVFADGAELVYDVSGGRPRLTTSTDEISGARP"
     gene            578..1156
                     /locus_tag="AC16VR_13955"
     CDS             578..1156
                     /locus_tag="AC16VR_13955"
                     /inference="COORDINATES: protein motif:HMM:NF047247.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase family protein"
                     /protein_id="HIR6012310.1"
                     /translation="MRLPYLLLLALITAMSFPGVGAEPPPAATEFPVPVVEAAGDFNG
                     VLARTAQVYIGGQPDASALTRMRALGVDTIVNLRTAAEMTNRQQVPFDEKAKAESLGM
                     RYVHIPAGGPKSPYSPSAVDQLAAAIAGTEGKVLLHCASGRRASHLWVAYLVRHEGLT
                     LEEAMSHGRAVNFGELPLEGFLGEPISFELAR"
     gene            1169..1618
                     /locus_tag="AC16VR_13960"
     CDS             1169..1618
                     /locus_tag="AC16VR_13960"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR6012311.1"
                     /translation="MLDKGDTAPDFELPDQAGEPIKLATLLDSGPVVLYFYPADFTAV
                     CTREACAFRDRHEDLSNLDVRIVGISPQNQASHRRFAETYGLPFPLLCDTSKKVIRAY
                     GVDGPLGLGVRRVTYLIGRDRIIANRAVSDIFLGSHLALIEEVLGQE"
     gene            complement(1664..2092)
                     /locus_tag="AC16VR_13965"
     CDS             complement(1664..2092)
                     /locus_tag="AC16VR_13965"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIR6012312.1"
                     /translation="MSDRRVAPERIAAGLLLAAFWLPLAVCTYLALHPSPPDAVFRLS
                     DVLLHGFAFTYLTLALALAHGHRHRLLPGLWMLGYGVLIELLQSFETSRSAEVGDVLV
                     DVAGIGLGLLAYGWAGAFLRALAVRIASLVVGAGASNADS"
     gene            complement(2067..3107)
                     /gene="hemE"
                     /locus_tag="AC16VR_13970"
     CDS             complement(2067..3107)
                     /gene="hemE"
                     /locus_tag="AC16VR_13970"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HIR6012313.1"
                     /translation="MTKTFLQACRGEATPHTPIWLNRQAGRYMPEYHQVKGDMPSLDF
                     FKNPERAARATLDAQRILGVDAAILFADLLPILEPMGLRLDYQPGVGPVFDNPIRSTA
                     DVDGLVSLPAEEATPYIAETVTNILEELPGEVALIGFAGAPFTLASYAIEGRGSRNYV
                     FVKKLMYEHEAVWHRLLDKLVEQVASYLHLQIRAGVDAVQLFDTWVGCLSVADFRRYV
                     LPHTRRLIQAIKEGSGGRVPVIYFGTGNSHLLADVVGAGPDVLALDWRVPLGETWKAL
                     GCTSIQGNLDPIVLCADRETVAEQAQVVLDQAAGRNGHIFNLGHGIIPETPVDNVKFL
                     VDFVHERSARSA"
     gene            complement(3113..4360)
                     /locus_tag="AC16VR_13975"
     CDS             complement(3113..4360)
                     /locus_tag="AC16VR_13975"
                     /EC_number="2.7.7.-"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR6012314.1"
                     /translation="MALRNLHLRAIPFRYAGYSGLALTILALQGTPRPAWALGVGAAG
                     CLIWTIAAHGAGRLAQTRGTSTVPFALGLHLAEALLAGALIGWLSLPWLPLLVAITVL
                     LASNAAQAGWWLAWRAGIAVAVGIGAGRLLAVAPVMSSTPAADLTASVLLLGYTVGLG
                     LTSFAKAQRLHAAQQDLARRALVTERLNSRLGRYLPPPVRARVRRRPDERCGLERRWL
                     TIAYVDVVGFTELAGRLAPEELAVVVNDYFAASGRLFASSGGTLAGLQGDGLLAYFGN
                     ATAATRGRAAVDCVESCLQVSRLLRELSECWQRRGYVTTLTTRCGVASGYCTVGDWGE
                     ERLDFTVIGAPVNLASRLQAHAGHDGILICEPTAALVGQVFPLGAERELVLRGIGKVK
                     ARRLESALVDPPPASAKVPRPEV"
     gene            complement(4457..5650)
                     /gene="fabV"
                     /locus_tag="AC16VR_13980"
     CDS             complement(4457..5650)
                     /gene="fabV"
                     /locus_tag="AC16VR_13980"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019820659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabV"
                     /protein_id="HIR6012315.1"
                     /translation="MIITPRIRGFICTTAHPAGCAAHVKEQIDYVSGQAALVGGPVVR
                     NALVIGASGGYGLASRIVAAFGCGAATLGVSLEKMPSETRTASAGWYNNVAFEEAAAA
                     AGLYAKTLDGDAFSDEMKGRVIDTLKADMGPIDLLIYSLASPVRQHPRTGELHRSVIK
                     PLGQALHMKSVHVDRGEVVEVDLEPADPEETAATVAVMGGEDWEFWIDALKDAGLLAP
                     GFRTLAFTYIGTELTWPIYWEGTLGKAKEDLDRAAGAISERLEDLEGNARVAVLKAVV
                     TQAMSAIPVVPLYASLLFKVMKERGVHEDPIQHIHRMFATQLCTGGALRLDDAGRIRM
                     DDLEMTDAVQEDVRDRWERVTTENLSELGDLDGFREEFLKIFGFGIEGVDYDADLDPT
                     LGRAP"
     gene            complement(5655..6644)
                     /locus_tag="AC16VR_13985"
     CDS             complement(5655..6644)
                     /locus_tag="AC16VR_13985"
                     /inference="COORDINATES: protein motif:HMM:NF013663.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HIR6012316.1"
                     /translation="MSGRDVRRLAARASNMTHEAKRDMVARVAAGAQAMGASDIYVTR
                     EPFRIASLALEHMGLAAAVHVLQTPLTNGPADTEAAVAAFLAAGCRVIVSLGGDGTNR
                     AIARALGDAGAAGAGVDLIPLSTGTNNVFPVLAEPTAAGMVAGLIASGRLPDDDLRQT
                     AKVIHLSGTDARGRAVRDLALVDAVLLRDDHVGNLLPFAAQRLARIVLTRAEPDAVGM
                     SPIGGMLDVVEAGDDEGLLVEMGPGREFSAPLSPGAFQAVSVRSASRLPLDVTVPLAG
                     PGVIALDGDRDYKIAPREALTARIRRDGPRVVDIAAAMRRAVARGIMAPARNG"
     gene            complement(6688..8667)
                     /locus_tag="AC16VR_13990"
     CDS             complement(6688..8667)
                     /locus_tag="AC16VR_13990"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR6012317.1"
                     /translation="MTPRRIKGLCSPLGWILLVLIAGCARAPAPVPEPEAGRPGNVVV
                     SGVPAIPAGAAHGLMRYRNVRSAYLMGWADDGLVIATRFGETTQLHRLKTPLGAREQI
                     TFFSEPVLAAFLPTGAGALGAPGFVLSRDAGGSEHYQLFLFDWSTRHARMVSNGRARY
                     TNVLWSNRGDQYAYTTTERDGRRWDIHLQDLAGGIKVALETSDGGWRAVDWSPGDDRL
                     LLIKYVSATESYLYELELASGSLRPLLDESLAVAIGGARYAGNGESVYFTADLGAEFM
                     RLHLLELATNSVEVLTADVPWDVTEIAVTSDGAHLAMVTNEGGVSRLGVLRLPDHSPL
                     ALPEVPAGTIYGLRLSPDGRQLGFTVNAPTAPGDVYSIDLVGRTLTRWTRSEVGGLDP
                     SAFAAPSLVHYPTFDREGGARRKIPAFVYMPDGPGPHPVVIHLHGGPHSQYRPKFSAS
                     AQYYVKELGLAVVAPNFRGSSGYGKNYLKLDDGYLREDAVRDVGALLDWIAAQPRLDA
                     DRVAVSGGSYGGYMALASLIRYGDRLVAGVSRVGISNFVTFLENTRAYRRDLRRVEYG
                     DERDPQMRAFLEGISPLGQADRIDSPLLVIHGFNDPRVPASESEQIADAVSAAGTPVW
                     YVLAMDEGHGFRKKANSDYAAAVTALFLQRYLLDD"
     gene            complement(8680..9183)
                     /locus_tag="AC16VR_13995"
     CDS             complement(8680..9183)
                     /locus_tag="AC16VR_13995"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HIR6012318.1"
                     /translation="MHVALIWAMARNRVIGRDNRLPWRLPRDMQFFVATTMGKPVIMG
                     RRTFESMKAPLAGRTNIVVTSDTGYRREGVLVAHSLEAALDLAQAQCRADDVEEAMVI
                     GGAEIYRVALPIATRLYVTLIDADVEGDTFFPEIDLDAWREVSREAFPADAGNGLPFS
                     IVVFERP"
     gene            complement(9190..>9837)
                     /locus_tag="AC16VR_14000"
     CDS             complement(9190..>9837)
                     /locus_tag="AC16VR_14000"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014576119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HIR6012319.1"
                     /translation="VVLRLIIHELLWFLQGSTNIKYLTDHNVHIWDEWADEEGNLGPV
                     YGSQWRSWPTPDGGTIDQIAAVVQSLRTNPNSRRHIVSAWNVAEVDSMALPPCHTMFQ
                     FYVAQGRLSCQLYQRSADIFLGVPFNIASYALLTMMIAQVCDLQPGEFIHTFGDAHLY
                     ANHLEQADEQLSRVPLPLPTVTLNPEVRDIFGFRYEDFTLTNYRCHGTIKAPIAV"
BASE COUNT         1793 a         3330 c         3182 g         1532 t
ORIGIN      
        1 acacacgccg gcgacaagaa agacggggtg ctttccggac agtttctcat ggagatgctc
       61 ggcgagcgtg tgacgctcaa agccggcgac atccttcacg tgccggccgg cgtggtgcat
      121 tgtgcaagcg ttctggggga agagccggtg gtcagcctgg acgccacaaa actttgagcc
      181 aggcgtcagg cagcgcctgc acggctctga tcgggggcaa ggtggcctcc attcgcgacg
      241 tccccgtccc ctccgcaccc tcatcgttgg gttctttgga gatcgagctc aagaacgggc
      301 gcgtgatcca cgcccgccct gatgcaacgg gaagttgcgc gattctgacg gggccggcac
      361 cttccgagcc gcgccgctgg cgcgacctga ctacggacct tcccttcgcc tttggccgcg
      421 acgccgtgat ttcgctggcc gagcccatcg aaggggagca gacgatcgtc ggtctgaggg
      481 tagtattcgc tgacggcgcc gagctcgtgt acgacgtgag cggcggccgc ccaagactga
      541 cgacatcaac tgacgaaatt tcaggagcgc gtccgtaatg cgactgccat acctcctgct
      601 cctcgccctg atcaccgcga tgagcttccc cggcgtcggt gccgaacccc cgccggctgc
      661 aaccgagttc cctgtccccg tcgtcgaggc cgccggcgac ttcaacggcg tgctggcccg
      721 cacggcgcag gtctacatcg gtggccagcc ggacgcctcg gcactcaccc gcatgagggc
      781 gctcggcgtc gacaccatcg tcaacctgcg gaccgcggcc gagatgacca accggcagca
      841 ggtaccattc gacgagaaag caaaggcaga atcgctgggt atgcgttacg tccacatccc
      901 ggcgggcggg cctaaaagcc cctactcgcc ctcagcagtg gaccagctgg ccgccgcgat
      961 cgccggcacc gagggcaagg tcctcctgca ctgtgcttct gggcgacggg cgagccacct
     1021 ctgggtagcc tatctggtga ggcacgaggg cctcacgctg gaagaggcca tgagccacgg
     1081 cagggccgtg aacttcgggg agctgccttt ggaagggttc ctgggagagc cgatctcttt
     1141 cgagctggca cgctgacgag gggtccccat gctcgacaaa ggtgatacgg ctccggattt
     1201 cgagctcccc gaccaggccg gcgagcccat caagctcgcc acgctgctcg atagcggtcc
     1261 cgtggtgctc tacttctatc cggccgactt cactgcggtc tgcacccgcg aagcctgcgc
     1321 gtttcgtgac cgccacgagg acctgagcaa tctggacgtc aggatcgtgg ggatcagccc
     1381 tcagaatcag gcgagtcatc ggcgcttcgc ggaaacctac ggcctgccat tccctctgct
     1441 gtgcgacacc agcaagaagg tcatccgcgc gtacggcgtc gacggccccc tcgggctcgg
     1501 agtacgccga gtgacctatc tcatcggccg ggaccgcatc atcgccaacc gggccgtttc
     1561 ggacattttc ctcggcagcc acctggcgct catcgaagag gtgctcgggc aagagtagct
     1621 ggccagactg gctcccgcgg acgtccaaga acgagcgctg gcgtcaggag tctgcattcg
     1681 acgcgcccgc gccgaccacc aagctcgcga tacgcaccgc cagcgcgcgc aggaaagcac
     1741 cggcccatcc gtatgcgaga agccccaggc cgattcccgc gacgtccacg agcacatcac
     1801 cgacctcggc ggaacgggac gtctcgaagc tctgcagcaa ctcgatcagc acgccgtagc
     1861 ccagcatcca gagcccgggc aaaagacggt gccgatgccc atgagccagc gcgagggcaa
     1921 gggtcagata ggtaaacgca aagccgtgca ggagcacgtc gctcaatcgg aacacggcgt
     1981 cgggcgggct cgggtgcagc gccaggtagg tgcagaccgc cagcggcagc cagaaggcgg
     2041 ccagcagcag ccccgccgca attcgctcag gcgctacgcg ccgatcgctc atgcacgaaa
     2101 tccaccagaa acttcacgtt gtccacgggc gtttccggaa tgatcccgtg cccgaggttg
     2161 aagatgtgcc cgttgcgccc cgccgcctgg tcgagaacga cctgagcctg ctcggcaacc
     2221 gtctcgcggt ccgcgcagag cacgatggga tcgaggttac cctggatcga agtgcagccg
     2281 agcgctttcc aggtttcccc cagggggaca cgccagtcga gggcgagcac atcgggcccg
     2341 gctccaacca cgtcggcaag caggtgcgaa ttgccggttc cgaagtagat cacggggacc
     2401 cggccacccg atccttcctt gatcgcctgg atcagccgcc gggtgtgggg tagcacgtag
     2461 cggcgaaaat ccgccacgga aagacacccc acccaggtat cgaacagctg caccgcgtcc
     2521 acgccagcac ggatctgcag gtgcagataa ctcgccacct gctcgaccag cttgtcgagc
     2581 aggcgatgcc agaccgcctc gtgctcatac atgagcttct tgacgaagac gtagttgcgc
     2641 gagccacggc cctcgatggc gtaagaggcg agtgtgaaag gcgcgcccgc gaagccgatg
     2701 agggcgactt cgcccggcag ctcctcgaga atgttcgtca cggtctccgc gatgtagggc
     2761 gtggcctctt ccgccggcag gctcacgagc ccgtcgacgt cggcggtgga tcgaatcggg
     2821 ttatcgaaga cggggccgac gcccggctga tagtccagac gcaatcccat gggctccagg
     2881 atgggcagca gatcggcaaa cagaatggcg gcgtccacgc cgaggatgcg ctgagcatcg
     2941 agcgtcgccc gggcggcgcg ttcaggattc ttgaaaaagt ccaggctcgg catgtcaccc
     3001 ttgacctgat ggtactcggg catgtaccgg cccgcctggc ggttcaacca gattggggta
     3061 tgaggcgtcg cttcgcccct gcaggcctga agaaaggtct tggtcatggt gctcacactt
     3121 ccggacgcgg caccttagca gacgccggcg gggggtcaac cagcgcgctt tcgagcctcc
     3181 tcgcctttac cttcccgatg cccctcagca ccagctcgcg ctccgcgccc agggggaaga
     3241 cctggcctac gagcgcagcg gtgggctcgc agatcagaat gccgtcgtgg ccggcgtggg
     3301 cctgcagcct gcttgccagg ttcaccgggg cgccgatgac ggtgaaatca agccgctctt
     3361 cgccccagtc tcccaccgtg cagtagccgc ttgccacccc acagcgggtc gtcagggtcg
     3421 tgacgtagcc gcgacgctgc cagcactcgc tcagctcccg caggaggcgc gacacctgca
     3481 agcaggactc cacgcaatcc acggcggcgc gaccccgggt ggctgcggtt gcgttgccga
     3541 agtacgcgag cagaccgtcc ccctgcagac cagccagggt gcccccggac gaggcgaaca
     3601 gccgtccgct ggccgcgaag tagtcgttga ccaccacggc cagctcctcg ggcgcaagcc
     3661 ggccagcgag ctcggtgaac ccgacgacgt ccacgtaggc aatggtcagc cagcgacgtt
     3721 cgaggccgca gcgctcgtcc ggccggcggc gcacccgggc gcgcaccggc ggcggcagat
     3781 aacggccaag cctgctgttg agccgttcgg tgaccagagc ccgccttgcc agatcctgct
     3841 gagcggcgtg caggcgctga gctttggcaa agctggtgag acccaacccg acggtgtagc
     3901 caagcagcag cacggaggct gtcagatcgg ccgctggcgt actgctcatg accggggcaa
     3961 cggccaggag acggcccgcg cctatgccca ccgccacggc gatgccggct cgccaggcaa
     4021 gccaccagcc ggcctgagcg gcgttgctgg caagcagcac ggtgatcgcc accagcaggg
     4081 gaagccaggg cagggacagc cagccgatca gcgcgcccgc cagcagcgct tctgcgagat
     4141 gcaggcccag agcgaatgga acagtggacg tgcccctggt ctgggcgagc cggcccgccc
     4201 catgtgcggc gatggtccag atcaggcagc cagcagcgcc gaccccgagg gcccaggccg
     4261 gcctcggcgt gccctgaagg gccaaaatgg tcagcgcgag acccgaatac cccgcgtagc
     4321 ggaaaggaat cgcgcgcagg tgaaggtttc gcagggccat ggtcgcgact ttggcgcgac
     4381 cgcattctcc gcgccaacga catcgaccag ctgcgctgtc agtttcctgg caggtggcgg
     4441 gaggacccgc tgcgtatcag ggggcgcgac ccagggtggg gtcgaggtcg gcatcgtaat
     4501 ccacgccctc gatgccgaac ccgaaaatct tcagaaactc ctcgcgaaag ccgtccagat
     4561 cgccaagctc ggacagattc tctgtggtga cccgttccca gcggtcgcgc acgtcctcct
     4621 gcaccgcatc ggtcatctcc aggtcatcca tgcgtatgcg ccccgcatcg tcgaggcgca
     4681 gggcgccgcc cgtgcagagc tgggtcgcga acatacggtg aatgtgctgt atcgggtctt
     4741 cgtgcacacc gcgctccttc atcaccttga agagcaggga cgcatagagc ggaacgaccg
     4801 gaatcgcgct catggcctgg gtcaccacgg cctttagcac cgccaccctg gcgttcccct
     4861 cgagatcctc cagtcgttcg gagatggcgc ccgccgctcg gtccagatct tctttggcct
     4921 tgcccagggt gccttcccag tagatcggcc aggtcagctc cgtaccgatg taggtgaatg
     4981 ccagcgtgcg gaaccccgga gccagaagcc cggcgtcctt cagcgcatcg atccagaatt
     5041 cccagtcctc gccgcccatc accgcaaccg tcgccgccgt ctcctccggg tccgcgggct
     5101 ccaggtccac ctcgaccacc tcgccacggt ccacgtgaac ggacttcatg tgcagcgcct
     5161 ggcccagcgg cttgatcacc gaccggtgca actcgccggt acgcggatgc tgccgaacgg
     5221 gggatgcgag gctgtagatg agcaggtcga tggggcccat gtcggccttg agggtgtcga
     5281 tgacgcggcc tttcatctcg tccgaaaacg catcgccgtc cagggtcttt gcatagagcc
     5341 cggccgcagc cgctgcctcc tcgaacgcga cgttgttgta ccagccggcg ctggcggtgc
     5401 gcgtctcgct cggcattttc tcaagagaaa cgccaagggt agcggcgccg cagccgaacg
     5461 cggcgacgat tcgcgaggcc aggccatatc cgccagaggc gccgatgacc agggcattgc
     5521 gcacgacggg cccaccgacc agggctgcct gcccggagac gtaatcgatc tgctctttga
     5581 cgtgcgcggc gcagccggcc gggtgcgctg tggtgcagat gaatccacga atgcggggcg
     5641 tgatgatcat gggtctaacc gttgcgggcg ggcgccatga taccccgagc gacggcacga
     5701 cgcattgccg cggctatgtc gacgaccctg gggccatccc gacggatacg ggccgtgagc
     5761 gcctcccggg gggcaatctt gtagtcccgg tcaccgtcca gcgcgatcac accgggccct
     5821 gccaggggga ccgtcacatc aaggggcaga cgcgaggccg agcgaaccga aaccgcctgg
     5881 aaagcgccgg gcgagagcgg cgctgagaac tccctgcccg gacccatctc caccaggagg
     5941 ccttcgtcgt cccccgcctc gaccacatcg agcatccccc cgatgggcga catgcccacg
     6001 gcatccggct cggcccgggt cagcacgatt cgagctagcc gctgcgcggc gaacggcagg
     6061 aggttgccca cgtggtcatc acgcagcagc accgcgtcca ccagcgcaag gtcccgaacg
     6121 gctcgtcccc gggcatcggt gcccgacaaa tgaattacct tggccgtctg ccggagatcg
     6181 tcgtcgggca gccgcccaga ggctatcagc cccgccacca tacccgcggc agtgggctcc
     6241 gccagaacgg gaaagacgtt gttggttccc gtggaaagcg gtatgaggtc aacgcccgca
     6301 cccgctgcgc ccgcatcccc aagggcgcgg gcgatggccc ggttcgtccc gtccccaccc
     6361 agcgagacga ttacccggca gccggcggca agaaaagccg ccaccgccgc ttcggtgtcg
     6421 gcaggtccat tggtcagcgg cgtctgaagc acgtgtacgg ccgcggcgag ccccatgtgc
     6481 tccaaagcca acgacgcgat tcgaaacggc tcccgggtca cgtagatgtc actggcgccc
     6541 atcgcctggg cgccggctgc cacccgcgcc accatgtcgc gtttggcctc gtgagtcatg
     6601 ttgcttgcgc gcgcggccag acgcctgacg tctcgaccgg acatgggatt gacgcagatg
     6661 gccaccccag ctcgtgagcc cggcccttca gtcatcgagc aggtagcgct gcaggaaaag
     6721 ggccgtaacc gcggccgcgt aatcgctgtt ggccttcttg cggaatccgt gcccttcgtc
     6781 catggcgagg acataccaga ccggcgtacc cgcggcgctc acggcgtcgg cgatctgctc
     6841 gctctccgat gcgggcactc gcgggtcgtt gaatccgtgg atcacgagca gggggctgtc
     6901 gatccggtca gcctggccga gcggagagat cccttcgaga aacgcgcgca tctgcggatc
     6961 ccgctcgtct ccgtactcca cccgccgaag gtcgcgacgg taggcgcgcg tgttctccag
     7021 gaacgtgacg aagttgctga tgcccacccg ggagacgccg gcgaccagcc ggtcaccgta
     7081 tcgaatcagg gaggccagtg ccatgtagcc cccgtaggag ccgccgctga cggcgacgcg
     7141 gtccgcatcc agacgcggct gggccgcgat ccagtccagc agagcaccta cgtcgcggac
     7201 cgcgtcctcg cgaagataac cgtcgtccag cttgaggtag ttcttcccgt aacccgacga
     7261 cccccggaaa ttgggcgcaa ccaccgccag gccaagctcc tttacgtagt actgggccga
     7321 tgcggaaaac ttcggccggt actggctgtg cggaccacca tgcaggtgga tgacgaccgg
     7381 atggggcccc ggcccgtcgg gcatgtagac gaaggcggga atcttccgcc gcgcgcctcc
     7441 ttcccgatcg aaggtggggt aatgcaccag gctcggcgcg gcaaaggccg acggatccaa
     7501 accgcccacc tcgcttcggg tccatcgggt aagcgttcgc ccaacgaggt caatgctgta
     7561 gacgtcgccg ggggcggtgg gcgcattgac cgtgaagccc agctgccggc cgtcgggcga
     7621 caatcgcagc ccgtagatgg ttcccgcggg cacttccggc agcgccaggg gcgagtggtc
     7681 gggcaaccgc aaaaccccca gccgcgagac cccgccctcg ttggtcacca tggccaggtg
     7741 ggccccatcc gaggtaacgg cgatctccgt gacgtcccag ggtacgtcag cagtcagcac
     7801 ctcgacactg ttggtcgcga gctcgagaag atgcagacgc atgaactcgg cgcccaggtc
     7861 ggccgtgaag tagacgcttt cgccgttccc cgcgtacctg gcgccgccaa tggccacggc
     7921 aaggctttcg tccagcagcg gtctcaagct gcccgatgcg agttcgagct cgtacagata
     7981 agactcggtc gccgaaacgt acttgatgag cagcaggcga tcgtccccgg gggaccagtc
     8041 taccgctcgc cagcctccat cagaggtttc cagcgcgacc tttattcccc cggcaagatc
     8101 ctggaggtgg atgtcccagc ggcggccgtc ccgctccgtg gtggtgtaag catactggtc
     8161 gcctcggttg gaccagagca cgttggtgta gcgggcccgg ccgttgctaa ccatgcgcgc
     8221 atgacgcgta gaccagtcga agagaaacag ctgatagtgc tctgagcccc ccgcgtcgcg
     8281 actcaagaca aagcccggag cgcccaaagc gccagcgccc gtgggaagaa aggccgccag
     8341 gaccggttcc gagaagaacg taatctgctc ccgcgcgccc agcggcgtct tcagccgatg
     8401 cagctgagtg gtttcgccga agcgggtggc gatgaccagt ccgtcatcgg cccagcccat
     8461 gaggtaggca gatcggacgt tccggtaacg catgagccca tgtgccgcgc ccgcgggtat
     8521 ggccggaacg cccgacacca ccacgttgcc gggccgccca gcctcaggtt ccggaaccgg
     8581 cgctggggcg cgcgcgcacc ccgctatcag gacgagcaga atccagccca acggcgagca
     8641 aaggcccttg atacgacgcg gcgtcacccg gcccgccacc tagggccgct cgaagacgac
     8701 gatggaaaac ggcagcccgt tgccggcgtc cgcgggaaac gcttcccggc tcacctcgcg
     8761 ccatgcgtcc aggtcgatct ccggaaagaa ggtgtcgcct tccacgtccg catcgatcaa
     8821 cgttacgtag agccgggtag caatcggcag cgccactcga tagatctccg cgccgccgat
     8881 gaccatggcc tcttcgacgt catccgcccg gcactgggcc tgcgccaggt cgagggcggc
     8941 ctccaggctg tgggcaacca gcactccctc gcgccgataa ccggtatcgc tggtgacgac
     9001 gatgttggtg cgcccggcca gcggcgcctt catggactcg aacgtccgcc gacccatgat
     9061 caccggtttg cccattgtcg tggccacgaa aaactgcatg tcccggggca ggcgccatgg
     9121 aagccggttg tcacggccga taacgcggtt gcgcgccatg gcccagatca gcgcgacgtg
     9181 caccgcgcgt cagaccgcaa tcggcgcttt gatggtgccg tgacagcggt agttcgtgag
     9241 ggtgaagtcc tcgtagcgga aaccgaaaat gtcccgcact tccggattca gcgtgaccgt
     9301 gggcagcggc agcggaacac gcgagagctg ctcgtccgcc tgctcgagat gattggcgta
     9361 gagatgcgcg tcaccgaagg tatgaatgaa ctcgccgggc tgcagatcac agacctgggc
     9421 gatcatcatg gtgagcagcg cataggaggc gatgttgaag ggaacaccca ggaagatgtc
     9481 ggcgctgcgc tggtaaagct ggcaggaaag ccggccctgg gccacgtaga actgaaacat
     9541 ggtgtggcag ggcggcagcg ccatgctgtc cacctccgcg acgttccagg cactcacgat
     9601 gtggcgccga gaattggggt tggtgcgaag agactgcacg acggcggcaa tctgatcgat
     9661 ggtgcctccg tccggcgtcg gccaggaacg ccattgagat ccgtagacgg gtcccaggtt
     9721 gccctcttcg tccgcccact cgtcccagat gtgcacgttg tgatcggtga ggtacttgat
     9781 gttggtgctg ccctggagaa accagagcag ctcgtggatg atcagcctga gcaccac
//
LOCUS       DBNHQR010000615         4970 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1516491, whole genome shotgun sequence.
ACCESSION   DBNHQR010000615 DBNHQR010000000
VERSION     DBNHQR010000615.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4970)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4970)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4970
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1516491"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..564
                     /locus_tag="AC16VR_14005"
     CDS             82..564
                     /locus_tag="AC16VR_14005"
                     /inference="COORDINATES: protein motif:HMM:NF013777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR6012320.1"
                     /translation="MEDQPNEVLMRGLLRAYYWMDESLQNGLQKAGYLPRTRTQTMIL
                     INISNGVTRAAELARVLGVSRQAIQQQINELERDDLVTQLPDPDDRRANRIVFSERGS
                     QLINAALATLRQSEQALAIRLGYETVNQLRRALMADWGPVIGERRAPGRKRSISNQND
                     "
     gene            complement(665..2521)
                     /locus_tag="AC16VR_14010"
     CDS             complement(665..2521)
                     /locus_tag="AC16VR_14010"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR6012321.1"
                     /translation="MLVALFSSGVTLAADHASRIEAHVESSATPWTGLQANDAEEDFT
                     FVVVADRTRGHRPGVFESAMPKVNLLQPAFVVSVGDLIEGYTDDPARLTGEWDEVEAF
                     VDQLDAPFFYAPGNHDMSNAVMSEAWRARFGPSFYHFLYKDVLFLVVNSELFGMVTNP
                     WVSVPGPWTQEEQMSYIERVLSRHADVRWTIVITHQPLWDVAEVHPDWLKVEALLGER
                     PYTVFAGHAHRYQRSLRHDRKYITLATTGGASPLRGALYGEFDHVAMVHMSSRGPTIA
                     NLMLDGIQPEDVTTEDSLRALRQLSDAVQVETEYLDGDLFTSGAVRYRIVNSGSAPLE
                     ATPIVLGNDVLRAQATPQAISVPPGGERRVALNLKSTHALAFESLPPARVTWSLRSML
                     LDRPVFVEIETALLPQRRFPLERISRLVRLDGDLEEWHLPYSVTRQGDIASPQTDPAD
                     ISFRFGIGYDDQNLYLAAEVIDDSIVASGELTAMDQDALIVSVDARPDPDRSANQPLV
                     EAMASGATSRMVIELLTLAPGRPAPVLEMLDGVRDAIASAVVRTSGGYAAEIAIPGEL
                     LSRQQGREWQVVRVGIHANDFDESERGSKSLHWQPYRFGSAAVVGTGTFMRR"
     gene            complement(2694..2987)
                     /locus_tag="AC16VR_14015"
     CDS             complement(2694..2987)
                     /locus_tag="AC16VR_14015"
                     /inference="COORDINATES: protein motif:HMM:NF014609.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HIR6012322.1"
                     /translation="MAKRAALGSRMQLTIVDVFAERRYAGNQLAVVRDASGLSTAEMQ
                     DIAREMNFSETAFVLESGSGHARARYFTSPPVRLGGEFDRALAGASSSIRAWR"
     gene            3087..3539
                     /locus_tag="AC16VR_14020"
     CDS             3087..3539
                     /locus_tag="AC16VR_14020"
                     /inference="COORDINATES: protein motif:HMM:NF015438.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HIR6012323.1"
                     /translation="MTGTVEGIYLAAKHGDSQRAVPAARLTIGVGLEGDRHANSKGGV
                     VTLIEAEAVERFIAETGLDITAADTGRNIATRGIGLNDLVGRQFRVGGVLMEGFELCE
                     PCATLGARLASRDVSAAAIVAAFTHSGGIRAFVRGTGTVAPGSAVEPA"
     gene            complement(3549..4139)
                     /locus_tag="AC16VR_14025"
     CDS             complement(3549..4139)
                     /locus_tag="AC16VR_14025"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6012324.1"
                     /translation="MTRQSGADGYPKQVRLPGGEEIEIRLMTGEDRDAVCEFARGLAE
                     EDLMFLRVDLTEPAVIDQWIRNLETGHSTSIVAYDGTGLVGYATVHRDPARWTRRVGE
                     IRVNVSPAYRAKGLGRTLTSQIFDLARSLGLRKLMANMTVDQSGAQAAFRRLGFVPEA
                     LLADYVEDRSGTPRDLVIMSYDIEGHTDQVAEPLRV"
     gene            complement(4159..>4970)
                     /locus_tag="AC16VR_14030"
     CDS             complement(4159..>4970)
                     /locus_tag="AC16VR_14030"
                     /inference="COORDINATES: protein motif:HMM:NF018657.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HIR6012325.1"
                     /translation="RRLRWTSHPFSEFREIRRAFGGTINDVVLAMVSEGAARYLDRRR
                     EETEGKYLRIMCPVSVRTEDEAGSLGNRVSAIFPLLPAWSMDAAERLRAVIGETERIK
                     RDEEAQALTFLSETMPSVPAVAMAPTLLVGTRFDPTALAGRMPLPVVPNLGTRLPLYG
                     FNFTCTNVPGVQVPQYLAGHEISNIIGILMLTGTLGYGMAVVSYNQNMRFNFVCEPRL
                     MPDLDVMVEAVEQVYQELLEEARRVNEPPAPPSRGGRSEKASRKSYDSPAA"
BASE COUNT          890 a         1660 c         1586 g          834 t
ORIGIN      
        1 gcactttgag aaacaatacc ggatactgcg tacctcggaa acgggccgca tgaattgtgt
       61 tgcgattgat tgaggattga catggaagat caacccaacg aagtgctgat gcgcggcttg
      121 ctgcgcgcct actattggat ggatgagagc ctccagaacg ggctgcagaa agcaggctac
      181 ctgcctcgca cccgcactca gacgatgatt ctcatcaaca tatccaacgg cgtgaccaga
      241 gctgcggagc tggcacgggt gctgggcgtg agccggcagg ccatccagca gcagatcaac
      301 gaactggagc gtgacgacct cgtcacccag cttccagatc cggacgaccg gcgcgccaac
      361 cgaatcgtct tcagcgagcg cggttcgcag ctgataaacg ctgcactggc aacactgcgc
      421 caatcagagc aggccctggc gatacggctg ggctacgaga cggtgaacca gctccgacgc
      481 gctctgatgg cagattgggg ccccgtgatc ggagagcggc gcgctcccgg acgcaagcgc
      541 tccatttcga accagaacga ctgaccggcg cgccgccttc cggcggcgct ctcgctcccc
      601 ccggacctgg cgtcgctgaa cgccgggcgg gtgcgactcc cccccaacat cgtcgccgag
      661 ccgatcacct tcgcatgaat gtgcccgtgc cgaccacggc cgccgagccg aatcgatagg
      721 gctgccagtg gagcgacttc gagccgcgtt cgctctcgtc gaagtcgttg gcatgaatgc
      781 cgacgcgaac gacctgccac tccctgccct gctggcgact gagcagttcc ccagggatgg
      841 cgatctcggc tgcgtatccg ccggacgttc gcaccaccgc gcttgcaatg gcgtcgcgca
      901 cgccatcgag catctccagc accggtgccg ggcgcccggg ggccagggtc agcagctcta
      961 tgaccatgcg cgacgtggca ccgctggcca tggcctctac tagaggctgg ttcgcagagc
     1021 ggtccgggtc gggacgggca tccaccgaga cgatgagcgc gtcctgatcc atcgcggtca
     1081 gctcaccgga ggcaacgatg gagtcgtcga ttacctcggc agcaaggtaa aggttctggt
     1141 cgtcgtagcc gatcccgaag cgaaacgaga tgtccgccgg atcagtctgc gggctggcaa
     1201 tgtcaccctg gcgcgtcacc gagtacggca gatgccattc ctcgaggtct ccgtccaggc
     1261 gaaccagacg gctgatgcgc tccagcggaa agcggcgctg gggaagcagc gcggtctcga
     1321 tctctacgaa taccggccga tcgagcagca tcgaccgcaa cgaccaggtg accctcgccg
     1381 ggggcaggct ctcgaaggcg agcgcgtgcg tggatttcag attcagcgcg acccgccgct
     1441 cccctccggg cggcaccgag attgcctggg gagtcgcctg ggcgcgcaga acgtcgtttc
     1501 ccaggacgat gggcgtcgcc tcgaggggag ccgaccccga gttgacgatc cggtagcgaa
     1561 ccgcgcccga ggtgaacagg tctccgtcga gatactcggt ttcgacctgc accgcgtccg
     1621 acagctgcct caacgcacgc agcgagtcct ctgtggtcac gtcctcgggt tgaatcccat
     1681 cgagcatcag attcgcgatg gtgggtccgc gcgaggacat gtgcaccatc gccacatgat
     1741 caaactcgcc gtaaagagcg ccccggagcg ggctggcccc gccggtggtc gccagcgtga
     1801 tgtacttgcg atcgtggcgt aacgatcgct ggtagcggtg cgcgtgaccg gcaaacaccg
     1861 tatacgggcg ttcaccgagg agcgcttcga cctttagcca gtcggggtga acctcggcga
     1921 cgtcccacag cggctgatgg gtgatgacga tcgtccagcg gacgtcggca tgcctggaga
     1981 gcacgcgctc gatgtaactc atctgctctt cctgggtcca gggccccggc acggacaccc
     2041 acggattcgt caccatgccg aacagctcgg agttgacgac caggaacagc acgtccttgt
     2101 agaggaagtg atagaaggac ggtccgaaac gggcgcgcca ggcctcggac atgaccgcgt
     2161 tggacatgtc gtgattgccc ggcgcgtaga agaagggcgc atcgagctgg tcgacaaagg
     2221 cctcgacttc atcccactcc cccgtgagcc gggccggatc gtccgtgtag ccttcaatga
     2281 gatctcccac gctgaccacg aatgccggct gcagcagatt gaccttgggc atggcggact
     2341 cgaacacgcc tggccgatga ccgcgcgtgc ggtcggccac cacgacaaag gtgaaatcct
     2401 cttccgcatc gtttgcctgg agtcccgtcc acggggtggc tgaggattca acgtgagctt
     2461 cgatgcggct cgcgtggtcg gcagccaggg tgactcccga ggagaacaac gcgacgagca
     2521 caaccaggcc cctcgcgggc cagctcgccc tgaccggcgg gcggctcgcg gacggccgtc
     2581 ctgtcttctg ccgacgcgaa tctttcaagc cagtcggccc tctgccacca gttgcacctt
     2641 gccgccgacg cggatctcgt ctcccacgtc gagatagagc cgccagggcc gtttcatctc
     2701 cacgccctga tcgacgacga cgcgcccgcg agggcccggt cgaactcccc gcccagccgc
     2761 acgggcgggg acgtgaaata gcgggcgcgg gcgtggccgc tgccgctttc caggacgaag
     2821 gcggtctccg agaaattcat ctcccgggca atgtcctgca tctctgcggt ggagaggccg
     2881 ctcgcatctc gcaccaccgc cagctgatta ccggcgtagc ggcgctcggc gaacacgtcg
     2941 acgatggtga gctgcattcg agaacccaag gcggcacgtt tcgccatcga gactagcacc
     3001 cgctctctgc atttcccaac ctccgcccat caggccgcgt gatgaacttg cgctaaactc
     3061 gctcatcagc accggagggc ttcccgatga caggcacagt cgagggcata tatctggccg
     3121 caaagcacgg cgattcccag cgggccgtgc ccgcggctcg actgaccata ggcgtcggcc
     3181 tcgagggtga tcgtcacgcc aactcgaaag gcggggtggt caccctgata gaggccgagg
     3241 cggtagaacg gttcattgcc gagacgggcc tggacatcac ggcggcggac accggtcgaa
     3301 acatcgccac ccgcggcatc ggcctgaacg acctggtggg caggcagttt cgcgtcggcg
     3361 gggtgctgat ggaaggcttc gagctatgcg agccctgcgc caccctgggc gcgcggctgg
     3421 cgagtcggga cgtgagcgcg gcggccatcg tcgctgcctt tacgcacagc gggggcattc
     3481 gcgccttcgt tcggggaacc ggcaccgtgg caccgggaag cgccgtcgag ccggcctgat
     3541 cgcatctctc acactcgcag cggttcggca acctgatccg tgtgaccctc gatgtcgtag
     3601 gacatgatca ccaggtcccg cggggttccg ctacggtcct ccacgtagtc cgccagcagc
     3661 gcctcgggca caaagcccag ccgacgaaag gccgcctggg caccggactg atccaccgtc
     3721 atgttcgcca tgagctttcg caagccgagc gaccgggcca ggtcgaaaat ctgcgacgtc
     3781 agcgtccggc ccagcccctt ggcgcggtat gcggggctta cgttcacccg gatctccccc
     3841 actctgcggg tccatcgggc ggggtcgcga tgcacggtcg cgtaccccac caggccggtg
     3901 ccgtcgtaag ccacgatcga agtcgagtga cccgtctcga ggttgcggat ccactgatcg
     3961 atcaccgcgg gctccgtcag gtccacgcgc agaaacatca ggtcttcctc ggcaagcccc
     4021 cgggcaaatt cgcaaaccgc gtcccggtcc tctccggtca tcaggcggat ctcgatctct
     4081 tcgccgccgg gcagccgcac ctgcttgggg tacccgtccg caccgctctg tctggtcatc
     4141 tttctacacc ctcctcagtc aggccgccgg cgagtcgtag gacttccggc ttgccttttc
     4201 cgaccggccg ccccggctgg gcggggccgg cggctcgttg acccggcgcg cttcctcgag
     4261 cagctcctga tacacctgct cgacagcctc caccatcacg tccagatcgg gcatcaggcg
     4321 cggctcgcag acgaaattga agcgcatatt ctggttgtag ctcacgactg ccatgccgta
     4381 cccgagggtg ccggtgagca tcagtatgcc gatgatgttg ctgatctcgt gaccggccag
     4441 gtactgcggc acctgaacgc ccggcacgtt ggtgcaggtg aagttgaacc cgtagagcgg
     4501 gagccgggtg cccaagttgg gcacgaccgg caacggcatt cgtccagcga gggcggttgg
     4561 gtcgaagcgg gttcccacca gcagcgttgg cgccatggcg accgcaggca cggacggcat
     4621 cgtctcgctg aggaacgtca gcgcttgcgc ctcctcgtcc cgtttgatgc gttcggtctc
     4681 tccaatcaca gcgcgcagcc tctcggcggc gtccatggac caggccggca gcagagggaa
     4741 aatggcggac acacgattgc cgaggctgcc ggcttcgtcc tcggtgcgca cgctcaccgg
     4801 gcacatgatt ctcaggtact tcccttcggt ttcctcgcgg cggcgatcca ggtagcgggc
     4861 ggccccctct gaaaccatgg cgagaaccac atcattgatg gtcccaccga aggcccgtct
     4921 gatctctctg aactcggaga acgggtggct ggtccagcgg agccggcgct
//
LOCUS       DBNHQR010000616         5864 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_567894, whole genome shotgun sequence.
ACCESSION   DBNHQR010000616 DBNHQR010000000
VERSION     DBNHQR010000616.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5864)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5864)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5864
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_567894"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2226
                     /locus_tag="AC16VR_14035"
     CDS             <1..2226
                     /locus_tag="AC16VR_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019339498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HIR6012326.1"
                     /translation="EIGLIGFTAAALYTMLAMVSYSPADPAFSFTGTGGSVDNLVGRS
                     GAWFADFTLYLFGRVAYILPVALVVIGVRLFRERSETFSWWLLALRCAGWLAVLIFGC
                     ILARIHFAPAAALPGGAGGILGEALVALGLPVFGWIGLTLIALTAVMIGAQAAAGFSW
                     LSVAELTGRWVHAFTANAVLWLDRQLDRWRRFREARSGAKEMMRAREEALGDERAKRG
                     SRAKPTIKPAASQKASTVAVQEPLFESTPSGDLPGLDLFDERQPGDDRGYSDESLEAI
                     SRLLELKLKDFGVEAEVVSVLPGPVVTRFEIQPAAGVKVQKISSLVKDLARSLAVISV
                     RIVEVIPGKSVVGIEIPNEHREVVRLKEVFSSAVFQDSKSPLTLALGKDIAGESVVAD
                     ISRMPHLLVAGTTGSGKSVGVNAMLLSILCKATPEDVRMILVDPKMLELSVYEGIPHL
                     LTPVVTDMKEAAHALNWCVAEMERRYKLMAALGVRNLAGYNRQIADAAKKGKTISDPL
                     WDADSEGEPAALGKLPLIVVFIDEFADMMMIVGKKVEQLIARIAQKARAAGIHLVLAT
                     QRPSVDVITGLIKANIPSRISFQVSSKVDSRTILDQGGAEQLLGHGDMLYLPPGSGLP
                     TRVHGAFVSDEEVHRVVSDWKQRGEPDYDNGVLSGGDDEPFLSIEPDPGSAEQEDALY
                     DEAVAFVLESRRASISSVQRKLRIGYNRAARLIEAMEAAGVVSAMNSNGSREVLVPNR
                     D"
     gene            2198..2827
                     /gene="lolA"
                     /locus_tag="AC16VR_14040"
     CDS             2198..2827
                     /gene="lolA"
                     /locus_tag="AC16VR_14040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein periplasmic chaperone
                     LolA"
                     /protein_id="HIR6012327.1"
                     /translation="MKSWFRIATDPVVWLVAALSCASAAADPRQALSERLAAMDVFVA
                     EFSQEIRGSQGQVLERSTGRFVLDRPLFKWEVDHPYPQTVVTDGDRLKVYDADLEQLT
                     IRPLAEALKDTPVSLLTREGVALGNEFQVVEVTDELGASYILTPNAPDTLYAQIRLHF
                     SDNALAALAITDHLGQFTEILFSTSAEPPVIQSSEFQLVVPPGTDVIGG"
     gene            2811..4100
                     /locus_tag="AC16VR_14045"
     CDS             2811..4100
                     /locus_tag="AC16VR_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015276857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HIR6012328.1"
                     /translation="MSLGDENAPLAERLRPNCLEEFVGQAHLLGAGKPLRTLAESGRL
                     HSMLLWGPPGTGKTTLAKLLAARAGARWISLSAVLSGVKDVRAAVAEAEALRGLGETT
                     VLFVDEVHRFNKAQQDAFLPHVEKGTVTFIGATTENPSFEVNAALLSRARVYVLKPLG
                     EEDLNRVIDRAAEQALNLPVTPDARALLTSLADGDARRMLNLLEVAAQLAEKEVDEAV
                     VTEAAGSRYRRFDKGGDAFYDQISALHKSVRGSAPDAALYWLCRMLDGGCDPLYVARR
                     VVRMATEDVGNADPRALQIALAAWDTYERLGSPEGELALAQAVLYLASVPKSNAAYVA
                     YGRAMGFVREGASEAVPMHLRNAPTRLMKDLGHGRDYRYAHDEPEHYAAGETYWPDGL
                     DPQAFYEPSDQGLEAKIRERLSRLRRLDQNTSGRAGR"
     gene            4097..4471
                     /gene="crcB"
                     /locus_tag="AC16VR_14050"
     CDS             4097..4471
                     /gene="crcB"
                     /locus_tag="AC16VR_14050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HIR6012329.1"
                     /translation="MTANALVWVALGGALGAVGRFLIGTWLFVPGQFPWATLTINIAG
                     SLGIGLLWGLGHQQPWFEQWGRYLIVVGLLGGFTTFSAFSLETVGLVEGQRWGGAVVY
                     VVASVVGCIVAAWAGQRLVGYY"
     gene            4498..5787
                     /gene="serS"
                     /locus_tag="AC16VR_14055"
     CDS             4498..5787
                     /gene="serS"
                     /locus_tag="AC16VR_14055"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018405676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HIR6012330.1"
                     /translation="MLDIKVLRDDPESVRDRLAVKGYLLDVSRVQALESERKRLQELT
                     ETLQNERNTRSKAIGKAKAAGEDIAPLLAEIGDLGDRLDAAKADFDALQEAYQGFLRE
                     IPNLPDASVPAGNDENDNQEIGQWGERRSFEFEPADHVALTEAGGLDFDTAAKITGSR
                     FVVMHGPIARLHRALVQFMMDVHVIEHGYREVYVPYIVNAESLMGTGQLPKFAEDQFR
                     LEGSNEFYLVPTAEVPVTNIYRETILAADQLPVRHVCHTPCFRSEAGSYGKDTRGMIR
                     QHQFEKVELVMLVRPGDSWRALEELTDHAETILRKLELPYRSVVLCGGDLGFSAAKTI
                     DLEVWLPGQDRYREISSCSNFLDFQARRMLARYRHPESGKPELVHTLNGSGLAVGRTL
                     IAILENYQDGEGRVAVPDALRDYLAGAESLDLSAGAG"
BASE COUNT          957 a         1822 c         1997 g         1088 t
ORIGIN      
        1 gaaatcggcc tgattggatt cacggccgcc gccctgtaca ccatgctcgc catggtttcc
       61 tactcacccg ctgatcccgc gttctcgttc acgggcaccg gcgggagcgt cgacaatctg
      121 gtgggtcgca gtggcgcctg gttcgccgac ttcaccttgt atctgttcgg aagagttgcc
      181 tacatcctgc ccgttgcgct ggtggtcatt ggcgttcggc tcttcaggga acgcagcgaa
      241 acgttttcgt ggtggctgct cgccctgcgc tgcgccggct ggctggcggt tctgatcttc
      301 ggctgcatcc tcgcgcgcat tcactttgct ccggcggcgg ccttgcctgg cggtgcgggt
      361 ggcatcttgg gggaagccct ggtcgccctc gggctgcccg tgttcggctg gatcgggctt
      421 acgctcatcg cgctgaccgc ggtgatgatc ggcgcccagg cggccgccgg tttctcctgg
      481 ctgagcgtcg cggagctgac gggaaggtgg gtgcacgcct ttaccgcaaa cgccgtgctc
      541 tggctcgatc gacagctgga ccgctggcgt cgttttcgcg aagcccgctc gggcgctaag
      601 gagatgatgc gtgcccggga ggaagccctg ggcgacgagc gcgcgaagcg cgggagccgc
      661 gcgaagccga ccatcaaacc cgccgcgtcg cagaaagcgt cgacggttgc cgtgcaggaa
      721 ccgcttttcg aatccacgcc cagcggcgat ctgccgggat tggacctttt cgacgagcgt
      781 cagcccgggg acgatcgcgg gtactcggat gaatccctcg aggcgatctc gcgactgctc
      841 gagctcaagc tcaaggactt cggcgtagag gccgaggtgg tctccgtgct gcccggcccc
      901 gtggtgacgc ggttcgagat acagccggct gcgggggtca aggtccagaa aatcagctcc
      961 ctggtgaagg acctggcccg ttccctggcg gtgatcagcg tgcggatcgt ggaggtgatt
     1021 cccggcaaat ccgtggtggg catcgagatc cccaacgagc accgcgaggt ggtgcggttg
     1081 aaggaggttt tcagctcggc cgtctttcag gactcgaaat cgccgctcac cctggccctg
     1141 ggcaaggaca tcgctggcga gtcggtggtg gccgacatct cccgcatgcc tcacctgctg
     1201 gtggccggca ccacgggttc gggtaagtcc gtcggggtca acgccatgct gctgagcatc
     1261 ctctgcaagg ccaccccgga agacgtccgg atgatcctgg tggatcccaa gatgctggag
     1321 ctgtcggtgt acgaggggat tcctcacctc ctcacgccgg tggtgacgga catgaaggag
     1381 gcggcgcacg cgctcaactg gtgcgtggcc gagatggaac gacgctacaa gctcatggcg
     1441 gcgctcggcg tgcgcaacct ggccggctac aaccgtcaga ttgcagacgc cgccaagaag
     1501 ggtaagacga tctcggatcc gctgtgggac gccgattcgg aaggcgagcc ggcggccctt
     1561 ggcaagctgc cgctcatcgt cgtgttcatc gacgagttcg ccgacatgat gatgatcgtc
     1621 ggcaagaagg tggagcagct gatcgcccgc atcgcccaga aggcgcgagc ggccgggatc
     1681 catctggtcc tggcgacgca gcgtccgtcg gtggacgtga tcacgggtct catcaaggcc
     1741 aacattcctt cgcgcatcag ctttcaggtg tcgtccaagg tggactcgcg cacgatcctc
     1801 gatcagggcg gggccgagca gcttctgggt catggggaca tgctctacct gccaccgggc
     1861 tctggactgc cgactcgtgt gcacggtgcg ttcgtctccg acgaggaggt gcaccgggta
     1921 gtctccgact ggaaacagcg gggcgaaccc gattacgaca acggcgtgct gtccggcggc
     1981 gacgacgagc cttttctctc catcgagccg gatcccggct cagcggagca ggaggacgcc
     2041 ctgtacgacg aggcggtggc cttcgtcctc gagtcgcggc gcgcgtccat ctcatccgtg
     2101 cagcgcaagc tccggatcgg ctacaaccgt gctgcgcggc tcatagaagc catggaagca
     2161 gccggcgtgg taagcgccat gaacagcaac ggcagtcgtg aagtcctggt tccgaatcgc
     2221 gactgacccc gtcgtctggc tcgtcgccgc gctatcctgc gcctcggcgg cggccgaccc
     2281 ccggcaggcg ctatccgaac ggctggccgc aatggacgtg ttcgtggcgg agttcagcca
     2341 ggagattcgc ggctctcagg gccaggtgct ggagcgctcc accggacggt tcgttctcga
     2401 ccggccgctg ttcaagtggg aagtcgacca cccttacccg cagacggtgg tgaccgacgg
     2461 agatcggctg aaggtttacg atgcggatct cgagcagctg acgatccgtc ctctggcgga
     2521 ggcgctgaag gacacgccgg tgtcgctgct tacccgcgag ggcgtagccc taggcaacga
     2581 gtttcaggtg gtggaggtca ccgacgagct tggtgcttcc tacatcctca cgcccaacgc
     2641 gcccgacacc ctgtatgcgc agattcgcct gcatttctcc gacaacgctt tggccgcgct
     2701 ggccatcacg gatcatctcg gtcagttcac ggagatcctc ttcagcacgt cggccgaacc
     2761 cccggtgata caatcgagcg agtttcagct cgtcgtgccg ccgggaaccg atgtcattgg
     2821 gggatgagaa cgcaccgcta gccgagcgcc tcaggccgaa ctgcctggag gagttcgtgg
     2881 gtcaggcgca tcttctgggc gccggaaagc ccctgcggac cctggcagag tccggccgcc
     2941 ttcattccat gctcctctgg gggccgccgg gtaccggcaa gaccaccctg gcgaagctgc
     3001 tggccgcccg ggccggtgct cgatggatca gcctctctgc cgtgctgagc ggggtgaaag
     3061 acgttcgggc cgccgtggct gaggcggaag ccttgcgggg attgggcgag accacggtgc
     3121 tgttcgtcga cgaggtgcac cggttcaaca aggcccagca ggatgccttt ctgccccatg
     3181 tggaaaaggg caccgtgacc tttatcgggg cgaccacgga gaacccatct ttcgaggtga
     3241 atgctgcgct cttgtcccgg gcaagggttt acgtgctcaa gcctttgggt gaggaagatc
     3301 tcaaccgggt catcgatcgg gcggcggagc aggcgctgaa cctgccggtc acccctgatg
     3361 ctcgcgcgct tctgacctcg ctggccgacg gcgacgcgcg gcggatgttg aacctgctcg
     3421 aggtggccgc tcagctggcc gagaaggagg tggacgaggc tgtggtgacg gaggccgccg
     3481 gcagccgcta ccggcggttc gacaaggggg gcgatgcctt ctacgaccag atctccgcgc
     3541 tgcacaaatc ggtgcggggc agtgctccgg acgccgcgct ctactggctg tgccgcatgc
     3601 tggacggtgg ttgtgatccg ctatacgtcg cccgcagggt ggtgcgcatg gcgacggagg
     3661 acgtcggcaa tgcggaccct cgggccctgc agatcgccct tgcagcgtgg gatacctatg
     3721 aacgcctggg ctcgcctgag ggggagctgg cgctcgccca ggcggtgctg tatctcgcca
     3781 gcgtgcccaa gagcaacgcc gcttacgtcg cttacggtcg ggctatgggc ttcgtgcgcg
     3841 agggggcgtc tgaagcggtg cccatgcacc ttcgcaatgc ccccacccga ctgatgaaag
     3901 atctgggtca cggccgtgac taccgttatg cccacgacga gcccgagcac tacgcagccg
     3961 gagagaccta ctggcccgac gggttggatc cccaggcatt ctacgagcct tccgatcagg
     4021 gcctcgaagc gaagatccgc gagcggctga gccgcctgcg ccggctcgat caaaacacgt
     4081 ccggccgggc gggaaggtga ccgcgaacgc tctggtctgg gttgctctcg gcggcgcgct
     4141 gggcgccgtg gggcggttcc tgatcggcac ctggctgttc gtgcccggtc aattcccatg
     4201 ggccacccta accattaaca tcgcgggctc gctcggcatc gggttgctct ggggtttagg
     4261 tcatcagcag ccctggttcg agcagtgggg tcgctacctc atcgtcgtcg gcctgctcgg
     4321 tgggtttacc acgttctcgg cgttctcgct ggaaacggtg ggcctcgtgg agggccagcg
     4381 ctggggcggc gccgttgttt acgttgtcgc gagcgttgtc ggctgcatcg tcgcggcctg
     4441 ggccggccag cgcctggtcg gctattactg agcatatcgg caagaggcga agacgccatg
     4501 ctagacatca aggtattgcg ggacgatccg gaaagcgttc gcgatcggct tgccgtcaag
     4561 ggttaccttc tggacgtctc tcgcgtgcaa gccctcgaaa gcgaacgaaa gcgtctgcag
     4621 gagctgaccg agacgttgca gaacgagcgc aatacccgat ccaaggccat cggcaaggcc
     4681 aaggcggcgg gtgaggacat cgccccgctg ctggccgaga tcggcgatct cggcgatcgg
     4741 ttggatgcgg ccaaagccga cttcgatgcg ctccaggagg cttatcaggg gtttctgcgg
     4801 gagattccca acctgccgga cgcgtccgtg ccggcgggca atgatgagaa cgacaaccag
     4861 gagatcgggc agtggggcga acggcggtcc ttcgagttcg agcccgccga tcacgtggcc
     4921 ctgacagagg ccggcgggct tgatttcgat acggcagcga aaatcactgg cagccgcttc
     4981 gtcgtcatgc acgggccgat cgcgcgtctg catcgggcgc tggttcagtt catgatggac
     5041 gtccacgtca tcgagcacgg ctaccgtgaa gtctacgtgc cgtacatcgt caacgcggag
     5101 tcgttgatgg gcacgggcca gctgccgaag ttcgccgagg atcagtttcg cctggagggg
     5161 agcaacgagt tttatctggt gcccacggcg gaggtgcccg tcaccaacat ctaccgggag
     5221 acgatcctgg cagccgatca gctgcccgtt cgtcacgtct gccatacgcc ctgctttcgc
     5281 agcgaggcgg gcagctacgg caaagacacc cgcggcatga tccgacagca ccagttcgag
     5341 aaggtggagc tcgtcatgct ggttcggcct ggtgattcct ggcgggcgct ggaggagctg
     5401 acggaccacg ccgagacgat tctgcgcaag ctggaattgc cctaccgcag cgtggtgctc
     5461 tgtggcggtg atctcggctt ttcggcggcc aagaccatcg acctggaggt ctggctgccc
     5521 ggacaggatc gctaccggga gatctcgtcc tgcagcaatt tcctcgactt ccaggcccgg
     5581 cgaatgctgg cgcgctatcg gcatccggag tccggtaagc ccgagctcgt gcacaccttg
     5641 aacggctcgg gcctcgccgt gggtcggacg ctcatcgcga tcctggagaa ttaccaggat
     5701 ggcgagggca gggtggcggt acccgatgct ctgcgcgact acctggcggg ggccgaatcg
     5761 ctggacctct ctgccggtgc cggttgagag ccggctctta gatccgtgga ttacctgccg
     5821 ctctttctcg acctcaagca gcgcccctgc ctcgtggtgg gtgg
//
LOCUS       DBNHQR010000617         3126 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1580410, whole genome shotgun sequence.
ACCESSION   DBNHQR010000617 DBNHQR010000000
VERSION     DBNHQR010000617.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1580410"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="AC16VR_14060"
     CDS             <1..737
                     /locus_tag="AC16VR_14060"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR6012331.1"
                     /translation="DPGDETTLGRPAGDVLAGLEDGLDGLKIGIPENVFWEEVDAEVA
                     EAVLASADVLRSLGARVERMAFPAADGARLLNPRGLVIAAEAYAVNRRFMDDHYDELD
                     PVVATRMIQGKGVAAADYIENLLQWQALRVEAMKQMAGVDALICPSTMIPATPLADCD
                     CDLDRYVERNLAYLRNTSIGNVLNLCGLSVPCGFTAAGLPIGAMICGSPFAEATVLRI
                     GHAYQRATDWHQRVPPLDWAAGVQSS"
     gene            complement(791..1381)
                     /locus_tag="AC16VR_14065"
     CDS             complement(791..1381)
                     /locus_tag="AC16VR_14065"
                     /inference="COORDINATES: protein motif:HMM:NF016269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HIR6012332.1"
                     /translation="MFRTHRTATLVTAILMMLISGSAAADKYDETVELFRNAGDSNEF
                     FAKSYGYAVFPTIGKGGFGIGGARGKGRVYVGGNHVGNTTMTQLTIGFQIGGQGFSQI
                     IFFQDERSFREFSTGNFEFGANASAVAITAGASASASTSGATAGASGGKKDATTAGTA
                     YYKGMKVFTIAKGGLMYEASIGGQKFSYRPAGGDQS"
     gene            1552..2901
                     /locus_tag="AC16VR_14070"
     CDS             1552..2901
                     /locus_tag="AC16VR_14070"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HIR6012333.1"
                     /translation="MAGSSREQWSSRFGFLMASIGFAVGLGNIWRFPYITGENGGGAF
                     VIVYLLCAFGIGVPILMAEVMIGRRGRLSPPGSMREVAVAEGRSRQWQWAGGLNLATA
                     FIIEVIYCVVAGWVLYYLYQAVTGGFVGVDAGSAQAGFAQLMDDTGTMLFWTVVGLVV
                     TGLIIYAGVEKGIERAVRILMPTLFGLLVLLAVYNVFAGGFGQALDYLLSADFSKLNA
                     ATVLIAVGQAFFSIGVALAGMMTFGAYLPRDVSIGQSVLVIVFADTLVALTAGLVVFP
                     AVFHFGLDPAGGPGLIFQTLPVAFGQMPGGQLVSILFFLLLSVAAVTSMVGLLEPLTS
                     WLEEHRGLSRHGSAVLVLGSVAALSVLSILSYNVLTGFRVFGRDLNGVFDYLSNQIML
                     PLGGFLIALFVGWFVSKATSRDELDIPDSRYFQTWHALIRYVVPPAVLAILVFGVMS"
BASE COUNT          469 a          968 c         1044 g          645 t
ORIGIN      
        1 ccgacccggg cgacgaaacc accctcggcc gcccggcggg cgacgtgctg gccgggttgg
       61 aagacggttt agacgggctg aagatcggaa taccggaaaa cgtgttctgg gaagaggtgg
      121 atgccgaggt ggctgaagcc gtgctggcca gcgccgatgt gctcagatct ctgggcgcgc
      181 gcgtcgagcg aatggccttc cccgccgcgg acggcgccag gctgctcaat cccaggggtc
      241 tcgtcatcgc ggcggaggcc tatgccgtga accgccgttt catggacgac cactatgacg
      301 agctggatcc ggtagtcgcc acgcgcatga tccagggcaa gggcgtcgct gccgccgact
      361 acatcgaaaa tctcctgcag tggcaggccc tgcgcgtgga agcgatgaaa cagatggcag
      421 gggtggatgc gctcatctgc ccctcgacga tgattccggc gaccccgctg gccgactgcg
      481 actgcgatct cgaccgctac gtggaacgca atctggcgta tctgcgcaat accagcatcg
      541 gcaacgttct gaatctctgt gggctcagcg tcccctgcgg atttaccgcc gcggggcttc
      601 ccatcggcgc gatgatctgc ggctctccat tcgccgaggc cacggtgctg cggatcgggc
      661 acgcctatca gcgggctacg gactggcacc agcgggtgcc accgctggac tgggcggcgg
      721 gcgtgcagtc gagttgacga gggcgcggcg acgggccgtc gcgcgatgcc agctggtagc
      781 ggtagtcaga tcaggactgg tcgccgccgg ccggtcggta ggagaacttc tggccgccga
      841 tgctggcttc gtacatcaga ccacccttgg cgatggtgaa caccttcatg cccttgtagt
      901 aagccgtgcc ggcggtggtc gcatccttct tgccaccgct tgcccccgcc gtcgctcccg
      961 aggtggacgc gcttgccgat gcgcccgcgg tgatggccac ggcgctggcg ttggctccga
     1021 actcgaagtt gccggtggag aactcgcgga aggaacgctc gtcctggaag aagatgatct
     1081 ggctgaaacc ctgcccaccg atctgaaagc cgatggtgag ctgtgtcatg gtggtgttgc
     1141 ccacgtgatt gccgccgacg tagacccggc ctttgccccg ggcgccgccg atgccgaagc
     1201 cacctttgcc gatggtgggg aacacggcgt agccgtagct cttcgcgaag aactcgttgc
     1261 tgtcgccagc gttgcggaac agctccacgg tttcatcgta cttgtccgct gcagcgctgc
     1321 ctgagatgag catcatcagg atggcggtga ccagggtggc ggttcgatga gttcgaaaca
     1381 ttcagacgac tccttgaaga aagcagttct cgagcggccg gaaggatagc acttcccagc
     1441 ttgttcgaaa ggataatccc tgcaactgaa ccgcgcctga accgacctcg gccgtgcatc
     1501 aaggggggct tcgaggttag attagcccgt ctacttgacg agagctgtgc catggccgga
     1561 tcgagccggg agcaatggtc gtcgcgcttc gggtttctga tggcctccat cggctttgcc
     1621 gtgggtctcg gtaacatctg gcgctttccg tacatcacgg gcgagaacgg cggcggcgcg
     1681 ttcgtgatcg tctatctgct ctgcgctttc ggcatcggcg tgccgatact gatggccgag
     1741 gtgatgatcg ggcgccgggg gcggctcagc ccgccgggat ccatgcgcga ggtcgccgtg
     1801 gcggagggtc gctcccgtca gtggcagtgg gcgggcgggc tgaatctcgc caccgccttc
     1861 atcatcgagg tgatttactg cgtcgttgcg ggctgggttc tctactatct ttatcaggcc
     1921 gtcaccggcg ggttcgtcgg tgtcgatgcg ggctcggcac aggccggctt tgcacagctc
     1981 atggacgaca ccggcaccat gctgttctgg acggtagtcg gcctggtcgt taccggcctc
     2041 atcatctatg ccggcgtgga gaagggcatc gagcgcgcgg tgcggattct catgccgacg
     2101 ctgttcggcc tcctcgttct gctcgcggtc tacaacgtgt ttgccggcgg tttcggccag
     2161 gccctcgatt acctgctctc ggcggacttc agcaagctga acgcggccac ggtcctgata
     2221 gctgtgggcc aggccttctt ctccatcggc gtagcgctgg cgggaatgat gacgttcggc
     2281 gcctacctgc cgcgcgacgt ttccatcggt caaagcgtcc tggtgatcgt gttcgccgac
     2341 accctcgtgg cgctgacggc ggggctggtg gtctttcccg cggtgtttca cttcggtctc
     2401 gacccggccg gcggccccgg cctcatcttt cagacgctgc cggtggcttt cggccagatg
     2461 ccgggtgggc agctggtcag cattctcttc tttctgctgc tctccgttgc ggccgttacc
     2521 tccatggtag ggctcttgga gccgctcacg tcctggctcg aggagcaccg gggcctcagc
     2581 cggcacggca gcgcggtgct cgtgctgggt tcggtggcgg ccctgagcgt gctgagcatt
     2641 ctctcctaca acgtcctcac cggattccgc gttttcggtc gggacctcaa cggcgtgttc
     2701 gactacctgt cgaaccagat catgcttccg ctcggcggct ttctcatcgc gctgttcgtt
     2761 ggctggtttg tcagcaaggc gacatcgcgg gacgagctgg acattcccga ctcccggtac
     2821 ttccagacgt ggcacgcgtt gattcgctac gtggtgcctc cggcggtgct ggcaattctc
     2881 gtttttggcg tgatgagctg acgacgcgcg ccggcctgcg gcgtgggtcg atcgaagacc
     2941 ctgttggccc ggcgcgttcc tgcgtcaggc cgagccctgc tggaggagtt cctcgagcgg
     3001 atcgccgttt tcgtaacgct gcagcaactt ctcggggtcg aagcccacgc cgatggggtt
     3061 cgcggcaaag gcatctgaac gcatgaagcc ctgcaggtcc tccacggagt cgaggttctc
     3121 gacctg
//
LOCUS       DBNHQR010000618         6076 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_504110, whole genome shotgun sequence.
ACCESSION   DBNHQR010000618 DBNHQR010000000
VERSION     DBNHQR010000618.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6076)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6076)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6076
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_504110"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..1076)
                     /locus_tag="AC16VR_14075"
     CDS             complement(6..1076)
                     /locus_tag="AC16VR_14075"
                     /inference="COORDINATES: protein motif:HMM:NF007504.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HIR6012334.1"
                     /translation="MLIDRDALTRFVSEIFVRLDAEASDAQEVAAHLVSANLKGHDSH
                     GVGMVPTYVGNIRSGALALNTHARLVRDKGAVMLIDGQMGFGQVVGREAVELAIERVR
                     QTGVVAAGVRNCHHLGRIGHYGEICGRAGYVSMHFVNVVGHQPLVSPFGGRQARISTN
                     PFCCVVPRSDEPPVVLDMATSAIAQGKVRVAYMKGDKVPDGSLIDHEGKPTNDPSVMF
                     EDPRGSLGPFGHHKGYGLALMCELLGGGLAGEWTAQAPPPDRFTIVNHMLMVVLDPDL
                     FGGAEAFHREVEEMIAYLRSTMPAAGVDRVRVPGEPELESMAERAAGGIPIDDNTWAA
                     IRKSAQVSGMTEEEIAGFTGSG"
     gene            1157..2014
                     /locus_tag="AC16VR_14080"
     CDS             1157..2014
                     /locus_tag="AC16VR_14080"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020039.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR6012335.1"
                     /translation="MDKCAEGFSLIEGPVWDPDRGLLFSDVLVGGVFCLGGDGTVTTV
                     FEHRRGIGGMALHEAGGLIVSGRNLSFKPYDEGSTVTVLDRDEENGLVGFNDLTTDAE
                     GRIYAGSLGASPVFADGREPRAGDLYLVDLDGSARVVAKDVRLTNGLGFSPDGTTLYH
                     SDSGRRTVFRYDVQPDGSLSAKEPFVTPDNGVPDGLAVSRDGAVWVALARGGHGVAVY
                     EASGRLREFIEIPLPMCTSVCFGGEDLKDLYIVSGSAGAGSDRAGAVFRERVDVAGLI
                     MTPARVRLA"
     gene            2082..2912
                     /locus_tag="AC16VR_14085"
     CDS             2082..2912
                     /locus_tag="AC16VR_14085"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6012336.1"
                     /translation="MAIEPMDTGTEDLLAHQEDGVAVITLNRPQARNAMSAAMNAGLA
                     KALDEAERNPAVRCVVITGAGGAFCAGGDVKGMAARSQGGGPGPTVDERIHLQRLNQR
                     ATAGRIYQMPKPVLASLPGPAAGAGLALALAADLRIMADSAFITTAFAKVGFSGDFGG
                     SFFLSRLVGSGKAKELYFLSDRVSAAECERLGIANAVVPAEELEAQTMALARRLASGP
                     PVAYRYMKENFNRAAAGAGVLECLDLEATHHVHTGLTEDHRAAAKAFVEKTEVEFKGR
                     "
     gene            complement(2950..3927)
                     /locus_tag="AC16VR_14090"
     CDS             complement(2950..3927)
                     /locus_tag="AC16VR_14090"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HIR6012337.1"
                     /translation="MDIKDGFAGTIGNTPLIRMNRLSEETGCEILGKAEFLNPGGSVK
                     DRAALWMVREHERSGALKPGGVVVEGTAGNTGIGLVHVCNARGYRSIIYMPDTQSPEK
                     VQLLKTLGAEVREVPAVPYRDENNYQKQAGRCAETLENGVWANQFDNAANRLAHHEST
                     GPEIWDQTEGRVDAFTCATGTGGTLAGVSRFLKERREDVRIVLADPMGSALYNWVTTG
                     ETTMSQGPSITEGIGNSRVTDNLAGTCIDDAVQVTDRDAVNMVYRELREEGWFFGSST
                     GINLCAAVQVAHSMGPGHTIVTVLCDTGAKYQSRLFNPEWLAERGLTPG"
     gene            4018..5184
                     /locus_tag="AC16VR_14095"
     CDS             4018..5184
                     /locus_tag="AC16VR_14095"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR6012338.1"
                     /translation="MKKADLQDRWLGDEGENGVNPLRIAALMARVRKEVDVGLLPAAL
                     VALARNGALVLFETMGAADNDSLFCVFSATKAITSAAAWILMQEGRLKEDELVADIIP
                     EFAANHKHDITVGQLFVHTAGFPSAPFKPLDWNDRARRLERFAQWQLNWPPGSRYEYH
                     PTSSMWVIAEIIERRANTTYQEFVRERIALPLGLPELYVGLPATENERVVPNVHVGDA
                     LTRADYELLGLPEPPVTEVTEEAILKFNRPEVRAVGVPGGGGIMSAADLALFYQGLLH
                     GGLGEEVWSAATLADARRVRSGELRDPVMGHRANRALGLVIAGDPQRNLRGFGKTNSP
                     ETFGHNGAGGQLAWADPATGLSLAYCTAGHDRNRIRQGRRGVAISSLAAVCCED"
     gene            complement(5707..>6076)
                     /locus_tag="AC16VR_14100"
     CDS             complement(5707..>6076)
                     /locus_tag="AC16VR_14100"
                     /inference="COORDINATES: protein motif:HMM:NF039797.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6491 family protein"
                     /protein_id="HIR6012339.1"
                     /translation="LACSIAGTVLLYASSGLALPPDSPIGPRAESIVEGTVQAPVSSI
                     PAANRIEGWARVDDKRVVLEVSPQTRYLLTLRQGCIGLGFARNVSVTMSNNTIYAGFD
                     AITADGEHCEIRTINPLVTP"
BASE COUNT          993 a         1944 c         2055 g         1084 t
ORIGIN      
        1 tctcttcagc cggaacctgt gaacccggcg atttcctctt ccgtcatgcc ggacacctgg
       61 gccgatttcc ggatcgcggc ccaggtgttg tcgtcgatgg gtatgccccc ggccgcgcgc
      121 tcggccatgg actcgagctc gggttccccg ggcacccgca ccctgtcgac gccggcagcc
      181 ggcatcgtgc tgcgcaggta ggcaatcatc tcctctacct cccgatggaa ggcctccgcg
      241 ccgccgaaca ggtccgggtc cagcacgacc atgagcatgt ggttgacgat ggtgaaccgg
      301 tccggaggag gtgcctgggc cgtccactct ccggcgagcc cgccaccgag aagctcgcac
      361 atcagggcga gcccgtaacc cttgtgatgt ccgaatggcc ccagcgagcc ccgggggtct
      421 tcgaacatca cgctgggatc gttggtcggt tttccttcgt ggtcgatcag ggatccgtcc
      481 ggcaccttgt ctcctttcat gtaggccacc cggaccttgc cctgagcaat ggcgctggtt
      541 gccatgtcga gcaccacagg gggctcgtcg ctcctcggca ccacgcagca gaaagggttg
      601 gtgctgatgc gcgcctggcg cccgccgaac ggcgagacca gcggttggtg tcccaccacg
      661 ttgacgaagt gcatcgacac gtagcccgct cgcccgcaga tctcgccgta gtgcccgatg
      721 cggcccaggt ggtggcagtt gcgcacgccg gccgcgacga cgcccgtctg cctgacccgc
      781 tcgatggcga gctcgacggc ttcgcgcccc accacctggc cgaaacccat ctgcccgtcg
      841 atgagcataa cggcgccctt gtcccttacc agcctggcat gggtgttcag cgcgagagcg
      901 cctgagcgga tattgccgac gtaggtcggc accatgccga ccccatggga atcgtggcct
      961 ttgagattgg cggacaccag atgggcggcg acctcctgcg cgtcgctggc ttcggcgtcg
     1021 agccgaacga agatttcgct gacgaagcgc gtcagtgcat cgcggtctat gagcatggtg
     1081 gcttctcatc gtgttggaac gctcagtgtt gcctctttaa agagcctgtg acaaggtctg
     1141 acgagagggg gttggcatgg acaagtgcgc ggaagggttc agcctgatcg aagggccggt
     1201 ctgggatccg gaccgcggcc tgcttttcag cgacgtcctg gtcggtgggg tcttctgcct
     1261 cggcggcgac ggcacggtga cgacggtttt cgagcatcgt cggggcatcg gcggcatggc
     1321 cctgcacgaa gccggtggcc tgatcgtcag cggccggaat ctgtctttca agccctacga
     1381 tgagggctcg acggtcaccg ttctcgatcg cgacgaggag aacggcctgg tgggcttcaa
     1441 cgatctcacc accgatgccg aagggcgaat ctacgccggc agtctgggag cgagccccgt
     1501 gttcgccgac gggcgtgagc cgcgtgcggg ggatctctac ctggtcgatc tggacggcag
     1561 cgcccgggtc gtggccaagg acgttcggct gaccaacggg ctcgggttct ctccggacgg
     1621 taccacgctc taccactccg acagcgggcg ccgaacggtc ttccgctacg acgtgcagcc
     1681 ggacggttcg ctctcggcaa aggagccgtt cgttacgccg gataacgggg tgcccgacgg
     1741 gctcgcggta tcccgcgacg gcgctgtctg ggtggcgctt gcccggggcg gtcacggcgt
     1801 agccgtctat gaggcgtccg gccgactgcg tgagttcatc gagattcccc tgcccatgtg
     1861 caccagcgtg tgctttggcg gcgaagatct gaaggacctt tacatcgtct ccgggtccgc
     1921 gggcgccggg tctgacaggg cgggagccgt cttccgagag cgtgtcgatg tggccggcct
     1981 gatcatgaca ccggctcggg tgcggctcgc gtgacgaagg cgtcgccgtc gacctgcagg
     2041 caggtgcgtc ttcatcggtt agagcatagg agtttgcagg catggcgata gaacccatgg
     2101 acacgggaac cgaggatctc ctcgcacatc aggaagatgg cgtcgcggtg atcacgttga
     2161 accggcccca ggcacgcaat gccatgtcgg ccgcgatgaa cgcaggcctg gcaaaggctc
     2221 tggacgaggc cgagcggaat cccgccgtgc gctgtgttgt catcaccgga gccggcggcg
     2281 cgttctgcgc cggcggagac gtgaaaggca tggcggcgcg aagccagggg ggcggccccg
     2341 ggcccaccgt ggacgagcgc attcaccttc agcgcctgaa ccagagggcg acggccggcc
     2401 gcatttacca gatgccgaag ccggttttgg cgagccttcc cggaccggct gcaggagcgg
     2461 ggctggctct cgccctggca gccgatctgc gcatcatggc cgactccgcc ttcatcacga
     2521 cggccttcgc gaaggtgggc ttcagcggcg acttcggtgg cagcttcttc ctctctcgcc
     2581 tggttggcag cgggaaagcc aaggaactct actttctttc cgatcgggtg agtgccgctg
     2641 agtgcgagcg gctgggaatc gccaacgcgg tggtgccggc ggaagaactc gaggcccaga
     2701 cgatggcgct tgcgcggcgg ctggcgtccg gaccgcccgt ggcataccgg tacatgaagg
     2761 agaacttcaa ccgggccgcc gcaggtgccg gggtgctcga gtgcctcgac ctcgaggcca
     2821 cgcatcacgt gcacaccggt ctcacggagg atcatcgcgc ggcggcaaag gccttcgtcg
     2881 agaaaaccga ggtcgagttc aagggccgat gaggcgttcc tccaggagcc gcggcgctcg
     2941 ggcgcgtcct cagcccggtg tcagaccccg ttcggccagc cactcggggt tgaacagtcg
     3001 ggactggtac ttggcaccgg tgtcgcaaag cacggtgacg atcgtgtgtc ctggccccat
     3061 cgagtgggca acctgaacgg cggcgcacag gttgatcccc gtgctcgaac cgaaaaacca
     3121 accctcctcc cgcagctcgc ggtagaccat gttcacggcg tcccggtcgg tcacctgaac
     3181 cgcgtcgtct atgcaggtac cggccaggtt gtccgttacc cggctgttgc cgatcccttc
     3241 ggtgatggac gggccctggc tcatggtggt ctcgccggtg gttacccagt tgtagagggc
     3301 gctgcccatg gggtcggcga gaacgatgcg aacatcctcg cgtcgttcct tgagaaagcg
     3361 gctcacccct gccagcgtcc ccccagtccc ggtggcgcag gtgaaagcgt ccacccgtcc
     3421 ctcggtctgg tcccagatct ccggccccgt ggactcgtgg tgcgccaggc ggttggcggc
     3481 gttgtcgaac tggttggccc acaccccgtt ttccagggtc tcggcgcagc gtccggcctg
     3541 tttctggtag ttgttctcgt cgcggtaggg aaccgcaggc acctcccgga cctcggcgcc
     3601 cagggtcttc aagagctgaa ccttctccgg ggattgggtg tcgggcatgt agatgatcga
     3661 ccggtagcct ctggcgttgc agacgtgcac caggccaatc ccggtgttgc ccgcggtgcc
     3721 ctccaccacc acgccgccgg gcttgagcgc gccggacctc tcgtgctcgc ggaccatcca
     3781 cagcgcggcc cggtccttga ccgagccgcc aggattcagg aactctgcct tgcctaggat
     3841 ctcgcagccg gtctcttcgc tcagccggtt catgcggatc aacggggtgt tgccgatggt
     3901 gccggcgaag ccatctttga tgtccatggc cgattttagt caggatccga ttgaatgcgg
     3961 aagttgccat gggtgatcat cccggctcgc gggcaagttc aggagatgcg cacagacatg
     4021 aagaaggccg atctgcagga ccgctggctg ggcgatgagg gggaaaacgg ggtcaacccg
     4081 ctgcgcatcg ccgcgctcat ggcgcgggtg cgcaaagagg tggacgtggg gctgctgccc
     4141 gcggcgctgg ttgccctggc cagaaacggc gccctggtgc tgttcgaaac gatgggcgct
     4201 gcggacaacg actcgctctt ctgcgtcttc tcggcaacca aagccatcac gtcggccgcg
     4261 gcctggatcc tcatgcagga ggggcgcctg aaggaggatg agctcgtagc cgacatcatc
     4321 cccgagttcg ccgcgaacca taagcacgat atcaccgtgg ggcagctgtt cgtgcacacc
     4381 gccggctttc cgtccgcgcc cttcaagccc ctggactgga acgaccgcgc gcgccgtttg
     4441 gagcgttttg cccaatggca gctgaactgg ccgccgggca gccgctacga gtaccacccg
     4501 acctcctcga tgtgggtgat cgcagagatc atcgagcggc gcgcgaatac cacctatcag
     4561 gagttcgtcc gcgaacgcat cgccctaccc ctggggctgc ccgagctcta cgtggggctc
     4621 ccggcgaccg agaacgagcg ggtggtgccg aacgtgcatg tgggcgatgc cctgacgcga
     4681 gcggactacg agcttctcgg tctgccggag ccgcccgtga ctgaggtgac cgaggaagcg
     4741 atcctgaagt tcaaccggcc ggaagtccgc gcggtcgggg tcccgggcgg gggcgggatc
     4801 atgagcgcag cggatctggc gctgttctac cagggattgc tgcatggtgg cctgggcgag
     4861 gaagtctgga gcgccgccac ccttgccgac gcccgtcgcg tgcgcagcgg cgaactccgc
     4921 gacccggtga tgggccaccg cgccaatcga gctctgggcc tcgtcatcgc gggagatccg
     4981 caacggaatc tccgcggttt cggcaagacc aactccccgg agaccttcgg ccacaacgga
     5041 gccggcgggc agctcgcctg ggccgatccg gcgaccgggc tgtccctggc ctactgcacg
     5101 gccggtcacg atcgcaaccg gatacgccag gggaggcggg gcgtagcgat ctcgagcctg
     5161 gctgccgtgt gctgcgaaga ttgaccagga cgcaacaccg cgcgcaggaa ccgcttgatt
     5221 tgcttctcaa tctgctccct ggcgtcgggg agcgcggaac gaactgtgac agacgcctct
     5281 gtccccgcag ggcggctgaa ctaatctgcc gactacgctg attgccaccg gcacagcgag
     5341 acgcgagagg agtcaccggc ccgtgcggcg gtgaacttaa cggttacacc gggattcgct
     5401 gtcgattctt cggggcttcc ccgacgggtc gagcagtcgg cgagttccgg ttttacatcc
     5461 ggcgtcgacc cgatgtccgg gtcaaggatc tcccggcatc agcccgaccg ccaggacggc
     5521 ggaggcgaac agagcaagga ctgcggaaac ggtggttgcc ggtagttcga aggatttagc
     5581 cgaaaggatt tcggctccga agggacttcg gagtggtgca aatggatctc aggagcatgg
     5641 agcacccaat ctttcgacgg cacactgttg tctcccccgc gtctgaaagc ggcgcatccc
     5701 cgcgcctcat ggggttacca acgggttgat ggtgcggatc tcgcaatgtt cgccatcggc
     5761 ggtgatggcg tcgaacccgg cgtagatggt gttgtttgac atggtcacgg acacgtttcg
     5821 cgcgaatccc agaccgatgc atccctggcg cagggtcagc aggtatcgcg tctgcggact
     5881 gacctccagc acgacccgct tgtcgtccac tcttgcccag ccttcgatcc ggttggctgc
     5941 cgggatgctc gagacggggg cctgcacagt gccctcgaca atggactccg cgcgggggcc
     6001 aatgggagag tccggcggca gggcgagtcc ggacgacgcg tagagcagta ctgtcccggc
     6061 gattgagcaa gcaagt
//
LOCUS       DBNHQR010000619         3601 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1453635, whole genome shotgun sequence.
ACCESSION   DBNHQR010000619 DBNHQR010000000
VERSION     DBNHQR010000619.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1453635"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="AC16VR_14105"
     CDS             <1..251
                     /locus_tag="AC16VR_14105"
                     /inference="COORDINATES: protein motif:HMM:NF019500.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HIR6012340.1"
                     /translation="GYGDITPQTPLGQAVASLVMIMGYGIIAVPTGIVTLELSEASRR
                     AMNTRTCPGCSAEGHTGEATYCWHCGHHLFEPRAVAHD"
     gene            complement(267..710)
                     /locus_tag="AC16VR_14110"
     CDS             complement(267..710)
                     /locus_tag="AC16VR_14110"
                     /inference="COORDINATES: protein motif:HMM:NF016701.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR6012341.1"
                     /translation="MKVDGRCFCGAVTYDAEIDPDRVLICHCTDCQNHSGSAYRVVAG
                     VVNDSFRLLTGELRSYEKVADSGSLRSRTFCPECGTNIYACTVGEGTGFFGLRLGTIN
                     QRAELTPKLRVWCRSALPWSDDLSGLPAFPAQPDLDALDEIVHQS"
     gene            883..3561
                     /gene="acnA"
                     /locus_tag="AC16VR_14115"
     CDS             883..3561
                     /gene="acnA"
                     /locus_tag="AC16VR_14115"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010490109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HIR6012342.1"
                     /translation="MSLDTFKTRRTLTVGGREYDYFSLPEAQSAGAGNVEKLPMSLKI
                     LLENLLRFEDDNTVKVEDITALANWASGGAAAEEIAYRPARVLMQDFTGVPAVADLAA
                     MRSAVVSAGLDPTIINPLSPVDLVIDHSVMVDSFGTETAFAENVALEMQRNTERYRFL
                     RWGQKAFDNFRVVPPGTGICHQVNLEYLARTVWTREENGRTVAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPISMLIPEVIGFRLTGKLRDGITATDLVLTVVEMLR
                     RRGVVGKFVEFFGEGLDQLPLADRATIANMAPEYGATCGYFPIDRETIAYLELSGRPA
                     ETVELVEAYARANAMWRDADTPEATYTDVLALDLDSVVPSLAGPKRPQDRVAMKDLGR
                     VFDEALELQGRAGDSDARVPVPGTDYDLGHGDVVIAAITSCTNTSNPAVMLGAGLVAR
                     KALARGMQVKPWVKTSLAPGSKVVTEYLEKTAVQEDLDALGFNLVGYGCTTCIGNSGP
                     LPEHISAAIKQGDLVVSSVLSGNRNFEGRVHQEVRANWLASPPLVVAYAIAGTTRIDL
                     TRDPIALDDQGREVFLRDIWPSNEEIADLVAQVSAEMFERQYADVFTGPDAWRAIDVA
                     ESGTYGWENSTYIKQPPFFTVGGDLDQPAEVSDARILALLGDSVTTDHISPAGAIQPD
                     SPAGQYLQQHAVDVADFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGIEGGYTRHVPS
                     GDQVSIYDAAIRYQGEGTPLVVIAGKEYGTGSSRDWAAKGTRLLGVKAVIAESFERIH
                     RSNLIGMGVLPLQFREGEGRASLNLSGEEIVDVRLPDGVTEISPRQDIEVVIKRGTDT
                     RRVTVQSRIDTENEIAYFKSGGILQYVLNNLVREAG"
BASE COUNT          651 a         1125 c         1216 g          609 t
ORIGIN      
        1 tgggatacgg cgacataacg ccgcagacgc ctctgggtca ggcagtcgcc tctctggtca
       61 tgatcatggg ttacggcatc attgcggtgc ctaccggcat cgtgactctg gagctcagcg
      121 aagcttcacg tcgtgccatg aacacgcgca catgtccggg atgctccgcc gaagggcaca
      181 cgggagaagc gacctactgc tggcactgtg gacaccatct gtttgagccg cgggccgtgg
      241 cgcacgattg accgcacggg tcgaggtcag ctctgatgga cgatctcgtc cagggcatcc
      301 agatccggct gggcagggaa ggccggtaac cctgacaggt cgtccgacca tggcagcgcc
      361 gagcggcacc agactctgag cttcggcgtc agctcggccc gctggttgat ggtgcccagc
      421 ctgagaccga agaaaccggt accttcgccc accgtgcagg cgtagatgtt ggtaccgcac
      481 tcggggcaga aggtcctcga gcggagcgag ccgctgtcgg caaccttctc gtacgatctc
      541 agctcgccgg tcagcaaccg gaagctgtcg ttcaccactc cggccacgac gcggtaggcg
      601 ctgcccgagt gattctggca gtcggtgcag tggcagatga ggacccggtc ggggtcgatc
      661 tcggcgtcgt aggtcacagc gccgcagaag cagcgtccgt cgactttcat gaaggcatcc
      721 tcccaggcga atgtcccgcc aagactacca gagctgcgcg ctgcgggcgt atccacgggt
      781 ttacgccccg cttcgcgccc tacaaaacac tgctcaggag ccgggtaacg agtacaatac
      841 cgcccctttt tcctggcacc gaaaacaacg agcgaggcag ccatgagctt agataccttc
      901 aagacccgca ggacgctcac cgtcggcggt agggaatatg actatttcag cctgccggaa
      961 gcgcagtccg ccggggctgg gaacgtcgaa aagctcccga tgtcgctgaa gatcctccta
     1021 gaaaacctgc tcaggttcga ggacgacaat accgtcaagg tggaggacat cacggcgctg
     1081 gccaactggg caagcggcgg ggcggcggcc gaggagatcg cctatcgccc ggcgcgggtg
     1141 ctcatgcagg acttcacagg tgtgccggcg gtggcggatc tggccgccat gcgcagcgcg
     1201 gtggtgtccg ccggcctgga tcccacgatc atcaatccgc tgtctcccgt ggacctggtc
     1261 atcgatcact ccgtgatggt cgacagcttc ggaaccgaga cggcgtttgc ggagaacgtc
     1321 gccctggaaa tgcagcggaa caccgagcga taccggttcc tgcgctgggg ccagaaggcg
     1381 ttcgacaatt ttcgcgtggt tccgccgggc accggcatct gtcaccaggt gaacctggaa
     1441 tatctggcca gaaccgtctg gacccgggaa gagaacgggc gcaccgtggc ctaccccgac
     1501 actctggtcg gcaccgacag ccacacgacg atgatcaacg gcctgggcgt gctgggttgg
     1561 ggcgttggcg gtatcgaggc ggaggccgcc atgctggggc agccgatctc catgctgatt
     1621 cccgaggtga tcgggttccg gctcaccggc aagctgcgcg acggaatcac ggccaccgac
     1681 ctcgtgctca ccgtggtcga gatgctccgc cgcaggggcg tcgtgggcaa attcgtcgag
     1741 ttcttcggag agggcttgga ccagcttccg ctcgcggacc gggcgaccat cgccaacatg
     1801 gcccccgagt atggcgccac ctgcggctat ttccccatcg accgggagac catcgcctat
     1861 ctcgagcttt cgggtcggcc ggcggaaacg gtggagctcg tcgaggcgta cgcacgggcc
     1921 aacgcgatgt ggcgggatgc cgacaccccg gaagccacct ataccgacgt tcttgccctg
     1981 gatctggact ccgtggtgcc gagcctggcg gggcccaagc gcccccagga tcgtgtggcc
     2041 atgaaggatc tgggcagggt ttttgacgaa gcgctggagc ttcagggccg cgcgggtgac
     2101 tccgacgcac gtgttccggt cccgggcacg gactacgacc tcggccacgg ggacgtggtg
     2161 atcgccgcca tcacctcctg cacgaacacg tcaaaccccg cggtgatgct gggtgccggc
     2221 ctggtagcgc gcaaggcgct cgctcgcggc atgcaggtca agccctgggt gaagacgtcg
     2281 ctggcgccgg gttcaaaggt cgttaccgag tatctggaga agacggccgt ccaggaagat
     2341 ctggatgccc tcgggttcaa cctcgtgggc tatgggtgca cgacgtgcat cggcaattcc
     2401 gggcctctgc cggagcacat ctcggccgcc ataaagcaag gggatctggt ggtgagctcg
     2461 gtgctgtcgg gtaatcgcaa cttcgagggt cgcgtccatc aggaggtcag ggccaactgg
     2521 ctggcttcgc ccccgctggt ggtggcctac gccatcgctg gcacgacgcg catcgatttg
     2581 acccgcgacc cgatcgccct ggacgaccag ggcagagagg tgtttctccg ggacatctgg
     2641 ccgagcaatg aggaaattgc cgatttggtg gcccaggtct ccgcggagat gttcgagcgt
     2701 cagtacgcgg acgtgttcac cggtcccgat gcctggcgtg ccatcgacgt ggcggaatcg
     2761 ggcacgtacg gctgggaaaa ctccacgtac atcaagcagc cgccattctt cacggtgggc
     2821 ggtgatctcg atcagccggc cgaggtgtcg gacgcccgta ttctggcgct gctcggcgac
     2881 tcggtgacca ccgaccacat ctcgccggcg ggcgccattc agccggacag cccggcgggt
     2941 cagtacctgc agcagcacgc cgttgacgtg gcggacttca actcctacgg ctcgcgtcgc
     3001 ggcaatcacg aggtgatgat gcgcggtacc ttcgcgaaca tccggatccg caacgagatg
     3061 gtccccggga tcgaaggggg ctacaccagg cacgtgccgt caggggacca ggtgagcatc
     3121 tacgatgccg cgatccggta tcagggcgag ggaacgccgc tggtggtcat cgccggcaag
     3181 gagtacggca ccggatcgag ccgggactgg gccgccaagg gcacccggct gctgggcgtc
     3241 aaggccgtga ttgccgagag cttcgagcgc atccaccggt cgaacctcat cggcatgggc
     3301 gtgctgccgc tccagttccg cgagggtgag ggccgcgcgt ccctcaatct ctcgggagag
     3361 gagatcgttg acgttcgtct gccggacggc gtgaccgaga tctcgccgcg ccaggacatc
     3421 gaggtggtga tcaagcgcgg gacggatacc cggcgggtga ccgtgcagag caggatcgac
     3481 acggagaacg agatcgccta cttcaagagc ggcgggatcc ttcagtacgt gctgaacaac
     3541 ctggttcgcg aggcaggctg acggttgctc gaagagcgct ccggccggcg gcgcgccgtg
     3601 c
//
LOCUS       DBNHQR010000620         2851 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_250637, whole genome shotgun sequence.
ACCESSION   DBNHQR010000620 DBNHQR010000000
VERSION     DBNHQR010000620.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_250637"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="AC16VR_14120"
     CDS             <1..484
                     /locus_tag="AC16VR_14120"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6012343.1"
                     /translation="ERFDVVTCQFGVMFFPDKHRSYAEARRVLRAGGHYVFNVWDAWP
                     FNAFAEITHDVVAGFFPDDPPGFYRVPFGYHDVEVIRRSLLDAGFRAASAETVQLTSA
                     IPSAENFARGLVFGNPLFQEIEQRGGDADAIHAAVADAVARRLGSQMQIRAIVVHAQK
                     "
     gene            complement(514..1011)
                     /locus_tag="AC16VR_14125"
     CDS             complement(514..1011)
                     /locus_tag="AC16VR_14125"
                     /inference="COORDINATES: protein motif:HMM:NF016213.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HIR6012344.1"
                     /translation="MDSAMLIDVLLWILVVGLVIVGVAGLLLPALPGAPLVFAGLLIG
                     AWIDDFAYVSWGTLITLGIMAALTYAVDFAAGALGASRFGASPRAVWGAVIGGIVGIF
                     MGLVGIIIGPFVGAVIGELSAQRSLEEAGRAGLGATLGLVIGTAIKLTLAMAMVGIFV
                     LMRFL"
     gene            complement(1083..1997)
                     /locus_tag="AC16VR_14130"
     CDS             complement(1083..1997)
                     /locus_tag="AC16VR_14130"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6012345.1"
                     /translation="MQIGIHAPHLGRELDPVTVRSFVQECDTLGVHSLWVSDHVCWPA
                     DIASKYPYTEDGSFPATPEMGWLDPIGTLLFIAGCTDRVRLGTTVLILPYRPPVATAK
                     QLATLDVVSGGRLILGVGIGWMAEEAAVLGMPWDHRGRRSDEQLEIFRRLFTETEPSF
                     EGEFYSFPKVGFEPKPVQQPVPVWVGGSTPAAFRRVARFGQAFHAAFQPLAEVGREWR
                     AVQAACEEIGRDPSDLSLSLRLYLDPDSRMEPEKSVAGSAEQMRERLFELAAAGVGHV
                     VLDPVARGGVQGRLDAVRGFMTDVAPAS"
     gene            2122..2748
                     /locus_tag="AC16VR_14135"
     CDS             2122..2748
                     /locus_tag="AC16VR_14135"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6012346.1"
                     /translation="MLKLYLMPGSCALASHITLREAGADFELKVVDFMSGEQRTPEYR
                     KVNPKGRVPALVTDRGVLTETPAILLYVAQTHPEARLAPLDDPFELARLQAFTSYLCA
                     TVHVAHAHSRRGERWADDESAIEAMKRKAPGNMADCFQLIEDQFFEGPWLMGEQFTVA
                     DPYLFTITRWLKFHQIDSSGYPRVHDHLGRMGERPAVRAALEAEAAAA"
BASE COUNT          495 a          970 c          930 g          456 t
ORIGIN      
        1 tgagcgcttc gacgtggtga cgtgccagtt cggggtcatg ttttttcccg acaaacaccg
       61 ttcctacgcc gaggcccgcc gggtgctgcg cgcgggcggt cactacgtct tcaacgtctg
      121 ggatgcctgg ccgttcaacg ccttcgcgga gatcacccac gacgtggtgg ccggcttctt
      181 ccctgacgat ccccccggtt tctaccgcgt gcccttcggc taccacgacg tcgaggtcat
      241 tcgccgctca ctgctcgacg ccggcttccg ggccgcgtcc gcggagacgg tccagctgac
      301 gtctgccata ccctcggcgg agaacttcgc gcgaggcctg gtgttcggta acccgctgtt
      361 tcaggaaatc gagcagcgcg gcggcgatgc ggatgcgatc cacgcagctg tcgcggacgc
      421 cgttgcccgg cgtctgggca gccaaatgca gatccgcgcc atcgtcgtgc acgcgcagaa
      481 gtagacgtct gcctgataag gtgcccggcg ccgtcagaga aagcgcatca ggacgaagat
      541 gcccaccatg gccattgcca gggtcagctt gatggcggtg ccgatcacga gccccagggt
      601 ggcgccgagt ccggccctgc cggcctcctc cagggagcgc tgcgcggaga gctcgccgat
      661 gacggcgccg acgaagggcc cgatgatgat gccaaccagg cccatgaaga tgcccacgat
      721 gccgccgatg acggctcccc agaccgcccg cggcgacgcg ccgaagcgtg atgcgcccag
      781 ggcgccggcg gcgaaatcca cggcgtaggt gagagcggcc atgatcccca gagtgatgag
      841 ggtgccccag ctcacgtagg cgaaatcgtc gatccaggcg ccgatgagaa gtccggcgaa
      901 caccaggggt gcccccggca gggccggcag cagcagcccg gccacgccga cgatcaccag
      961 gcccacgacc agaatccaga gcagcacgtc gatgagcatc gcagagtcca tgggcctatt
     1021 gtggcgcgtc agtgctcacg gagaaaccgt tccgcagggg cctgcacggc cggcttcgct
     1081 cctcaggacg cgggggcgac gtcggtcatg aatcccctaa cggcgtccag gcgcccctgc
     1141 acgcccccgc gggcaaccgg atccaacacc acgtggccca cgccggcagc ggccagctca
     1201 aaaagcctct cgcgcatctg ctccgcgctc ccggccaccg acttttccgg ctccatgcgg
     1261 ctgtccggat cgagatagag ccgcagagac aggctcaggt ctgagggatc gcgcccaatt
     1321 tcctcgcaag ccgcctgcac cgcccgccat tcccggccga cctcggccag cggctgaaag
     1381 gcggcgtgaa aggcctgacc gaatcgcgcc acccgccgga acgcggccgg cgtggatccc
     1441 cctacccaga ccggaaccgg ctgttgcacc ggtttcggtt cgaagccgac cttggggaag
     1501 ctgtaaaact ctccttcgaa gctgggctcc gtttcggtga acagccgcct gaatatctcc
     1561 agctgctcgt cggaccggcg tccgcgatga tcccagggca tgcccaggac ggcggcctcc
     1621 tcggccatcc agccgatgcc gacgccgaga atgagccggc cgcccgagac aacgtccagg
     1681 gtggccagct gtttggctgt ggccaccggc gggcgatagg gcaggatcag caccgtggtg
     1741 cccagacgta cccggtcggt gcatccggca atgaagagca gcgttccgat ggggtccagc
     1801 cagcccatct cgggcgtggc gggaaaggat ccgtcttcgg tgtagggata tttcgaggcg
     1861 atgtctgccg gccagcagac gtggtcgctt acccacaggg agtggacgcc gagggtgtca
     1921 cactcttgga cgaatgaacg aaccgtcacc ggatcgagct cgcgcccgag gtgcggcgcg
     1981 tgaatgccga tctgcattgt ggagcctccc agtggcttcg gtttgagtct agcagaccgc
     2041 agtatcgggg acgcgacgtg agcccccagc cgagctaaag tgaagatccc atctgcggtt
     2101 gtgggctcac agggagatca gatgctgaag ctctacctca tgccgggcag ctgcgccctg
     2161 gcctcgcaca tcacacttcg cgaggcgggc gccgacttcg agctcaaggt ggtggatttc
     2221 atgtccggcg agcagcgcac gccggagtac aggaaggtga atcccaaggg ccgcgtcccc
     2281 gcgctcgtca cggaccgggg cgtgctgacg gagacgcccg ccatcctgct gtatgtcgcc
     2341 cagacccatc ccgaagcccg attggccccg ctggacgacc ctttcgagct tgccaggctg
     2401 caggcgttca ccagctacct ctgcgccacg gtgcatgttg cccacgccca cagccgccgc
     2461 ggcgagcgct gggcggacga cgagagcgcc atcgaggcga tgaagcgcaa agcgcctggc
     2521 aacatggcgg actgcttcca gctcatcgag gatcagttct tcgagggccc ctggttaatg
     2581 ggcgagcagt tcaccgtggc ggatccctat ctcttcacca ttacccgatg gctgaagttc
     2641 catcagatcg actcgtccgg ctatccccgg gtgcacgacc acctcgggcg catgggggaa
     2701 cgtcccgccg ttcgcgcggc gcttgaggcc gaggccgccg cggcttgagt cgccggaacg
     2761 cccccgcgcc cgcctgtggg gtgtgcggct tcgcctcaat gtggtgaatc tcgccatctt
     2821 cgaggtgggc ctgtgagggc aaccatctga a
//
LOCUS       DBNHQR010000621         2626 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_568280, whole genome shotgun sequence.
ACCESSION   DBNHQR010000621 DBNHQR010000000
VERSION     DBNHQR010000621.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_568280"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1685)
                     /locus_tag="AC16VR_14140"
     CDS             complement(<1..1685)
                     /locus_tag="AC16VR_14140"
                     /inference="COORDINATES: protein motif:HMM:NF016292.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HIR6012347.1"
                     /translation="MVNSTSVLGRLSFGLIAIAAAAAILLFSDLEARKDRTVSDADDA
                     AKVWRVALVQLAPVQPVDEGVRGILAGLAARGYVDGDRLEVIQYNAQGDLAVANAIAA
                     QVTSAGLDMIITASTPSLQTVANANRSRANPTVHIFGISSDPYGAGVGISREDHLDHP
                     PYMAGLGSLPPVRELFDLVRTINPAARRVGLVWNPTEANSEAATHLARAAAAEFGMEL
                     VEGNAESSTAASEVAQSVISRGIDVLWVSPDSTVYTAIDALIEAARRGGVPVVTSLPG
                     SVGRGSLLDLGARYEDIGYVQGLLAADVLEGRDPASVPVENWIPVDLHLNLTVLDGIS
                     RSWRIGPDLVASASVVIDDSGTRERGLPKPEPPEWLRWDRLAEGGQVPNVDLVMYSES
                     PVAEQSIEGVRLGLAQGGFDPDLRLNLRVHSAQGDMPTLVSIIDAIRPTSTELIMTLS
                     TPALQNALQRVRGIPIVFGMVSNPFIVGAGISDTDHVPTATGAYLNQPLESALDIVAE
                     LFPEARTLGTLFTPAEVNSQYNRDRLLEVAKARGYSVENIGIATAADVLDASIA"
     gene            2076..>2626
                     /locus_tag="AC16VR_14145"
     CDS             2076..>2626
                     /locus_tag="AC16VR_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6012348.1"
                     /translation="MAHAKPLVVDMDSHVLEPPDLWQRYLEPKYRHRAIRIEQGADGI
                     ETLFVDDKALLSGRLAALGGAEHDAVRTFTDPDLTYLDGCPKASYDTQARVQLLDEWG
                     VDMGVVFPTIGILWDKEDDPELAMAYARAYNNWQWDFAKPALDRILPIAHVPLYDVDL
                     AYEELKRCLKLGFKGMFMAPEPV"
BASE COUNT          475 a          871 c          833 g          447 t
ORIGIN      
        1 gcgatcgaag catccaggac gtctgcggcc gtggcgatac cgatgttctc caccgagtag
       61 ccccgtgcct ttgccacttc tagaagcctg tccctgttgt actgggagtt gacttccgcc
      121 ggcgtgaaca gcgttcccag cgtccgggct tcggggaaga gctcggcgac gatgtccaac
      181 gccgactcga gcggttggtt caggtaggcg cccgtggccg tcggcacgtg atccgtatcg
      241 cttatgccgg ctccgacgat gaaggggttc gacaccatcc cgaagacgat cgggatcccg
      301 cggactcgct gcagcgcgtt ctggagcgcc ggcgtgctta gagtcattat cagctccgtg
      361 ctcgtgggcc tgatggcgtc gatgatgctg acgagggtcg gcatgtcccc ctgggccgag
      421 tgaacgcgca agttgagcct aagatcgggg tcgaagcccc cctgagcaag gcccagcctg
      481 accccttcaa tggattgctc cgcaaccggg ctctccgagt acatgaccag gtcgacgttc
      541 ggcacctgcc ctccttcggc gagccggtcc cagcgcagcc actcgggcgg ttcgggctta
      601 ggtagccctc gctcccgcgt gccggagtca tcgatcacga cgctcgcgct ggccacgagg
      661 tcgggtccga tgcgccagct gcgggagatc ccgtccagca ccgtaaggtt gaggtgcagg
      721 tcgaccggga tccagttctc caccgggacg ctcgcaggat cccgcccttc gaggacgtcg
      781 gcggcgagaa gaccctgcac atagccgatg tcctcatacc tggcacccag gtccaggagg
      841 gagcctcggc cgaccgaacc cggcagggaa gtcacgaccg gaacgccgcc gcgacgggca
      901 gcctcgatga gcgcatcgat ggcagtgtag acggtgctgt ccgggctcac ccagaggacg
      961 tcgatccccc gcgagatcac ggactgcgcc acctcgctgg ccgccgtcga tgactccgcg
     1021 ttcccctcca cgagctccat cccgaactcg gcggcggcgg cgcgggccaa gtgggtggcc
     1081 gcctcggagt tcgcctccgt cgggttccac acgaggccca cccggcgcgc ggcaggattg
     1141 atggtccgca ccaggtcgaa cagctcccgc acgggtggca ggcttcccag gccggccatg
     1201 tagggcgggt ggtccaagtg gtcctcacga ctgataccga ccccggcgcc gtagggatcg
     1261 gaagagatac cgaagatgtg cacggtcgga ttcgcccggg accggttggc gttcgccacg
     1321 gtctgcagtg agggggtcga ggccgtgatg atcatatcca ggcctgcgct ggtgacctgc
     1381 gcggcgatgg cgttggcaac ggccaggtcg ccctgggcgt tgtattggat cacttcgagg
     1441 cggtcaccat ccacgtaacc ccgcgccgcg agccccgcca gtatcccccg gaccccttca
     1501 tccaccggct gcacgggggc gagctgcacc agcgcgaccc gccacacttt tgcggcatcg
     1561 tcagcgtcgg aaaccgtccg gtcctttcgg gcctcgaggt cggaaaacag aaggatcgcg
     1621 gcggccgccg cgatcgcgat caacccgaaa gagagcctgc ccaacacgct cgtggagttc
     1681 accatcgaga atctacactt ccccccaacc tgtataagac atcttacgtt gggaaaacaa
     1741 aagcaaaaaa gctctggttg cgagaagaag ggcccgcccg gctaccagcc cagcatcgag
     1801 tacatcacgc accgggcttc cgcggaggcc gtcatggcct tctttccggg cctgacgcgc
     1861 gacgggctcc cagacggtga gggatgggcc gcgacgctgt acctcctcga gcgcggcggc
     1921 cgcgtcaccg ccaccgatcc ccaccgaagc cccgcttcgg atcgcagcga tcagctcgac
     1981 accctcggcc cttcgaccga ttcaaggcga attccgccaa cctcgccaga tacgggcgtt
     2041 atactttcgc gaacagccag tgatgggaga aacatgtggc ccatgcaaag ccgttggtag
     2101 tggatatgga ctcccatgtg ctggaaccgc cggatctgtg gcagagatac ctcgagccca
     2161 aataccggca tcgggccatc cgcatcgagc agggagccga tggcatcgaa acgctcttcg
     2221 tggatgacaa ggctctgctt tccgggcggc tcgcggcgct cggcggcgca gagcacgacg
     2281 ccgttcggac cttcacagac ccggatctga cctacctcga tggctgtccg aaagcctctt
     2341 acgacaccca ggcgcgcgtg cagctgctcg acgagtgggg cgtggacatg ggcgtggtgt
     2401 tccccaccat aggaattctg tgggacaagg aagacgatcc cgagctggca atggcgtacg
     2461 cgcgcgccta caacaactgg cagtgggatt ttgccaaacc agctttggat cgcatcctgc
     2521 cgatcgctca tgttcccctg tacgacgtgg atcttgccta cgaggaactc aagcgctgcc
     2581 tgaagctcgg cttcaagggc atgtttatgg cgccagagcc cgtgtg
//
LOCUS       DBNHQR010000622         5108 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1580989, whole genome shotgun sequence.
ACCESSION   DBNHQR010000622 DBNHQR010000000
VERSION     DBNHQR010000622.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5108)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5108)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5108
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1580989"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="AC16VR_14150"
     CDS             complement(<1..272)
                     /locus_tag="AC16VR_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012349.1"
                     /translation="MTPTDSGPATLRRFLPWLLPTALLAAHAAAADVRIGVDWNGDGL
                     IQTLSGSRVPQDAPSDSQPFVFWVNHDQDDLETGGETWPIARPDSNT"
     gene            complement(295..1074)
                     /locus_tag="AC16VR_14155"
     CDS             complement(295..1074)
                     /locus_tag="AC16VR_14155"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR6012350.1"
                     /translation="MRHWPPSRTSPIGAADIIFKAMLVPAVLLAVAACTREAEPRWLL
                     RALPGEYPEIVYFVPTTSRAVAMTIDDGLDPETTPLILETLKAHGASATFFVLSDSIA
                     GREVLLQRILYEGHEIGHHMTEDEVTVSLKPAAMAEKFHRAAQTLDAFAPITWFRPGS
                     GRYNDAVLELTRARGYRIALASVAPLDTVIHNSARMAGFVERMIEPGSVVVLHDVGER
                     GRRTAETLKLLLPKLKERGYAVVSLSRLDALAGMDTSATEE"
     gene            1382..2950
                     /locus_tag="AC16VR_14160"
     CDS             1382..2950
                     /locus_tag="AC16VR_14160"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007977712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004065 - arylsulfatase activity [Evidence
                     IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HIR6012351.1"
                     /translation="MKSSRRTSTTSILLALLFASMSAWSQDKPNILVIWGDDVGWSNI
                     SAYNLGMMGYQTPNIDRIAEEGALFTHYYAHQSCTAGRSSFILGQHPFRTGLLTIGMP
                     GADHGIPDWSPTIADLLKNHGYATGQFGKNHLGDRDEHLPTNHGFDEFFGNLYHLNAE
                     EEPETYHYPKDPEFRARFGPRGVIKSTADGQIQDTGPMTRKRMETADEEFLGSAMDFI
                     DRSVKADKPFFVWMNTTRMHVWTRLKAEAEGVTEMGLYPDGMAEHDGHVGLLLGQLDR
                     LGIADNTIVIYSTDNGAEKVTWPDGGTTPFHGEKGTTWEGGMRVPAVVRWPGTIKPGT
                     KIHDIMSHEDWLPTLLAAAGENDVVQKLKKGHRANGKRWRVHPDGYNFLPYLKGEADA
                     PREEIFYFGQNGDLDAVRWRDWKVSFATFDGNIATGIREVANWPVITHLRSDPFEEMH
                     YESSMYLRWMADNIWLFVPIQAKVQEFLSTMGDYPFQEGQSLNAAGINYRSLKALQIL
                     QQLNQKGVLSPPTN"
     gene            2952..4652
                     /locus_tag="AC16VR_14165"
     CDS             2952..4652
                     /locus_tag="AC16VR_14165"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIR6012352.1"
                     /translation="MVARRLRAAAALAALLLITGIATVEAASRPKLILQITVDQLRAD
                     LPTRYYERLGKGGFKYLWEKGIVFTNAHHAHANTETIVGHATLATGAHPSVHGMVGNL
                     WFDRETGFTTYNVEDGDHRLLTEAADVDDATEIDPTQRAARSEGRSPAAILVTTFADE
                     LRSNTNGRAKAIGVSVKDRGAISMAGHSGTAYWFSKATGGFVTSTYYLDRYPDWVSDF
                     NAAGPAAAYAGTAWELLREQDTYLFGEADDRPWETDVAGFGRVFPHAFGDPSSPYFAT
                     WLTLSPAGDRLVLDFAKRAIVEESLGQDATTDYLSVSFSSTDYVGHVFGPSSLEAEDN
                     ILRLDRTLAELFAFVAKQVGLADTLIVLSADHGAPDSPGYLNSLGIPGGYVDPDSWDK
                     ADAIDRIKARFGIEGALFEKYAHPYLYFSPEVAGNEQIDRGALEAAVIEELTKLPGVA
                     LAVSSTALGSASIPDSEIHRRVLRNHHPDRSGDVFIVFEPNWFINDFDGLTVASTHGS
                     PWRYDTHVPVVFAGARLRPAKIDRAVRTVDVAPTLSALLQIKAPSGSVGEPLVEVLRR
                     "
     gene            4654..>5108
                     /locus_tag="AC16VR_14170"
     CDS             4654..>5108
                     /locus_tag="AC16VR_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012353.1"
                     /translation="MNARGLIVVASMLLVGSVGWAEPDEYAVDAEDDFELAAQEMADA
                     MADGGFELDDAKFAGNPMQRLFKKWPEDLVVAPIPSRSPQLGFQLSVVGGYFLGERDE
                     AAGKRPSAIGGFAMASDNGSYAFGAGGKFHLLDDRLRLQAGAGYADIRY"
BASE COUNT          933 a         1640 c         1644 g          891 t
ORIGIN      
        1 gtgttggagt cgggcctggc aatgggccag gtttctccac cggtctccag atcgtcctga
       61 tcgtggttga cccagaacac gaaaggctgc gaatccgagg gggcgtcctg tggcacgcgg
      121 ctgccggaca gggtctggat caagccgtcg ccgttccagt cgacgccgat acggacatcg
      181 gctgccgcgg cgtgcgcggc aagcaaggcc gtggggagca gccagggcag gaagcgtctc
      241 aacgtcgccg gtccagagtc ggtcggtgtc acggtttcgg tcctgctttt cgcgtcattc
      301 ctcggtggca ctggtatcca tcccggcgag cgcatccagc cggctcaggc tgaccaccgc
      361 gtacccccgt tccttcagtt tcggtagcag cagctttaag gtttctgcgg ttctcctgcc
      421 ccgctcgccc acgtcgtgaa gcaccaccac gctgccgggc tctatcatcc gctcgacgaa
      481 gcccgccatg cgggccgaat tatgaatcac ggtgtccagc ggtgccaccg aggccagcgc
      541 gatccggtag ccccgcgcgc gcgtcagctc gagcaccgcg tcgttgtaac ggccggagcc
      601 cggccgaaac caggtgatgg gcgcaaacgc gtcgagcgtc tgcgcggcgc gatggaattt
      661 ctctgccatc gcagccggtt taagggagac cgtgacctcg tcctcggtca tgtggtggcc
      721 gatctcgtgg ccctcgtaga ggattctctg cagcagaacc tcgcggccgg caatgctgtc
      781 gctcaggacg aagaacgtgg ctgaggctcc gtgagctttg agcgtctcga ggatcagagg
      841 cgtggtctcg ggatccagac cgtcgtcgat agtcatggcc accgcccggg aggtcgtcgg
      901 cacgaagtag acgatctcgg gatactcccc aggcagcgcg cggagcagcc accgtggctc
      961 cgcttcccgc gtgcaggcgg caacggccag cagcacggcc ggcaccagca tcgctttgaa
     1021 gatgatgtct gctgcgccga tgggtgacgt gcggctcggc ggccagtgtc tcatcaggat
     1081 tgcgtccgga cttcctggga gtggcgagtg tgattcgacg gctctctatc gtgtcagagt
     1141 cgccgtcttc atgaaaccgg ccccccgtca tgaacttcgg ctccctgctg gttcggcctg
     1201 accgtcccac cgacaaactt gcaatcccga gaccgggcgc taggctgtgc ggttttgcca
     1261 agcgttggaa aagcgcagcg ggatcggccc tggtgcgcat tcaggaaccc gcggaagaag
     1321 aataaccaga cgctcaggcc ttcaccgact ctaactgcgc acggatttgg agatctcaca
     1381 gatgaaatcg agtagacgga cgtcgaccac gtcgatcctg cttgcgctgc tgttcgcctc
     1441 aatgtccgca tggtctcagg acaagccgaa catcctggtc atctggggtg acgacgtggg
     1501 ctggagcaac atcagcgcct acaacctggg gatgatgggc taccagacgc cgaacatcga
     1561 ccggatcgcc gaggagggtg cattgttcac ccattactac gcccaccaga gctgcacggc
     1621 aggacggtct tcgttcatcc tgggccagca cccgttccgc acgggcctgc tgaccatcgg
     1681 catgcccggc gccgatcacg ggatacccga ctggagcccg accatcgccg atctgctgaa
     1741 gaaccacggc tacgcgacgg gccagttcgg caagaaccat ctgggtgatc gggacgagca
     1801 cctgccgacc aatcacgggt ttgatgagtt tttcggcaac ctctatcacc tcaatgcgga
     1861 ggaagagccg gagacctatc actatccgaa ggacccggag ttccgggcgc gcttcgggcc
     1921 gcgaggagtg ataaagagca cggcggacgg ccagatccag gacaccgggc ccatgacgcg
     1981 aaagcgcatg gagacggcgg acgaggagtt tctcgggagc gccatggact tcatcgatcg
     2041 ttcggtgaag gccgacaagc ccttcttcgt ctggatgaac acgacgcgca tgcacgtgtg
     2101 gacgcgattg aaggcagagg ccgagggggt cacggaaatg gggctctacc cggacggcat
     2161 ggccgaacac gacggccacg tgggcctgct tctcggtcag ctggatcgcc tcggcatcgc
     2221 cgacaacacc atcgtcatct acagcaccga caacggcgcc gagaaggtca cctggccgga
     2281 cggcggcacg acgccgttcc acggcgagaa gggcactacc tgggaaggcg gcatgcgggt
     2341 gcccgcggtg gtgcgctggc cggggaccat caagcccggg accaagatcc acgacatcat
     2401 gtcccacgag gactggctgc cgacgctgct ggccgcggcg ggcgaaaacg acgttgtgca
     2461 gaagctgaaa aagggccacc gggccaacgg caagcgctgg agggtgcacc cggatggtta
     2521 caacttcctg ccttacctca agggcgaagc ggacgcgccg cgcgaggaga tcttctactt
     2581 cggccagaac ggcgatctgg atgcggttcg ctggcgggac tggaaggtca gctttgcgac
     2641 cttcgacggc aacatcgcga cgggtatccg ggaagtggcc aactggccgg taatcaccca
     2701 tctgcgttcg gatccgtttg aagagatgca ctacgagtcg tccatgtatc tccgctggat
     2761 ggcggacaat atctggctgt tcgtgcccat ccaggccaag gttcaggagt tcctctcgac
     2821 catgggcgac tatccgttcc aggagggcca atccctgaac gccgcgggca tcaactaccg
     2881 atcgctgaaa gcgctgcaga tccttcagca gctcaatcag aagggcgtgc tgtcaccgcc
     2941 gacgaactga cgtggtagcg cgtcgattgc gcgcagcggc cgccctcgcg gcgctgctgc
     3001 tgattaccgg catcgcaacc gtcgaggcgg ccagccggcc aaagctgatt ctccagatca
     3061 cggtggatca gctcagggcc gatctgccga cccgctacta cgagcggctc ggcaaaggcg
     3121 gcttcaagta tctgtgggag aagggcatcg tcttcacgaa cgcccatcac gcacacgcca
     3181 acacggaaac catcgtcggc cacgccaccc tggctaccgg cgcgcacccc tccgttcacg
     3241 gcatggtggg caatctctgg ttcgaccggg aaaccgggtt cacgacctac aacgtcgagg
     3301 atggggacca ccggctgctc accgaggccg ccgacgtcga cgatgccacg gagatcgatc
     3361 ccacccagcg cgccgcccgc agcgaaggcc gctcgcccgc cgcgatcctg gtgaccacct
     3421 ttgccgatga gcttcgcagc aataccaacg gtcgcgcgaa agccattggc gtctcggtca
     3481 aggatagggg cgccatctcc atggcgggtc attccggcac cgcgtactgg ttttcaaagg
     3541 cgacgggcgg gttcgtcacc agcacctatt acctggaccg ttacccggac tgggtttcgg
     3601 acttcaacgc cgccgggccc gccgcggcat acgccgggac ggcttgggag ctcttgcgcg
     3661 aacaggacac ctacctgttt ggggaggccg acgatcggcc ctgggaaacc gacgtggcgg
     3721 gattcggccg ggtatttccc cacgcctttg gcgaccccag cagcccctac ttcgccacct
     3781 ggctgaccct gagcccggcc ggtgaccggc tggtgctcga cttcgccaaa cgagccatcg
     3841 tcgaggagtc gctggggcag gacgcgacca ccgactacct gagcgtgagc ttttcctcca
     3901 ccgactacgt tggccacgtc ttcggaccgt ccagcctgga ggcggaagac aacattctcc
     3961 ggctcgaccg aaccctggcg gagctgttcg ccttcgtcgc caagcaggtg ggcctggccg
     4021 acacgctgat agtgctttcc gccgatcacg gcgcgccgga ttctcccgga tacctgaaca
     4081 gcctgggtat cccgggtggc tacgtcgacc cggacagctg ggacaaggct gacgccatcg
     4141 atcggatcaa agcgcgattc gggatcgagg gcgcgctgtt cgagaagtac gctcatccgt
     4201 atctctactt cagcccggaa gtcgccggca acgagcaaat cgaccgaggc gccctggaag
     4261 cggcggtgat cgaggagctg acgaagttgc ccggagttgc cctggccgtg tccagcacgg
     4321 ccctgggctc ggccagcatt cccgactcgg agatccatcg ccgcgttctg cgcaaccatc
     4381 atccggaccg ctccggcgat gtgttcatcg tgttcgagcc caactggttc atcaacgact
     4441 tcgacgggct cacggtggcc tccacccacg gttctccctg gcgctacgac acccacgtgc
     4501 ccgtcgtgtt tgccggcgct cggctccgcc cggcaaagat cgatcgtgcc gtgcgcacgg
     4561 tagatgtcgc gccgacgctg tccgcgctgc tgcagatcaa ggcgcccagc ggctccgttg
     4621 gcgagccgct ggtcgaggtg ttgaggcgat aggatgaatg cgcgcggact catcgtggtg
     4681 gcctccatgc tgcttgttgg ctcggttggt tgggccgaac cggacgagta cgccgtcgat
     4741 gcggaggacg atttcgagct ggccgcgcag gaaatggcgg atgccatggc cgatgggggc
     4801 ttcgagctcg acgatgccaa attcgccggc aaccccatgc agcgcctgtt caagaagtgg
     4861 ccggaggatc tggtcgtcgc gcccatcccc agccgatcgc ctcagctcgg cttccagctg
     4921 tccgtggtcg ggggatattt tcttggcgaa cgggatgaag ccgcggggaa gcggccgtct
     4981 gccatcgggg gcttcgccat ggcctcggac aatggcagct acgcgttcgg ggccggcggg
     5041 aaatttcact tgctggacga ccgcctgcgc ctgcaggcgg gcgcgggcta cgccgatatc
     5101 cgttacaa
//
LOCUS       DBNHQR010000623         4647 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_504621, whole genome shotgun sequence.
ACCESSION   DBNHQR010000623 DBNHQR010000000
VERSION     DBNHQR010000623.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4647)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4647)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4647
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_504621"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="AC16VR_14175"
     CDS             <1..468
                     /locus_tag="AC16VR_14175"
                     /inference="COORDINATES: protein motif:HMM:NF014543.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012354.1"
                     /translation="PDGRRYEGGFAGDAKSGKGVLEWPNGNRFVGDFAEDARSGLGIF
                     YWRDGTVYQGQYAQDKMHGYGIKEQPDGAMELQRWQEGALQFGKPLLADARCSLTIDS
                     QPWMFYSTQCINGLAHGQGLAVSLDGRLIVEEGRFVLGNLIEGEIRVLRLGES"
     gene            468..2732
                     /locus_tag="AC16VR_14180"
     CDS             468..2732
                     /locus_tag="AC16VR_14180"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR6012355.1"
                     /translation="MTLELPGFQVQAEIGHGATSRVYLAMHKQLSRLVALKILAEEYG
                     NDPAFRVRFLNESRIGSSLSHPNIVQVYDTGAHDNTLYLVLEYVRGGNLLQRVDRGMH
                     IRDLVRVVQNIGKALDYAHARGLVHRDVKPENILFREDGSAVLTDFGIAGFVDPDGPP
                     AKGGTVPGTPQYMSPEQAAGKALDGRSDLYSLGIVFFRVLTGDVPYKADTAVSLGIKH
                     LQEPIPRLPNHLAIFQDVVNRSLAKKPDQRFQSGQEFADALEALAASGQVPSLMIKAR
                     AVAPEEIRSLGSNVFARARDSGRGERQSAPERRPLTLGRAALAASLVAAVLAFGIVVV
                     EQPQWFTRMLAVTGIAEDPEVEDAWSNAQSLHQDPNQSLATIVAGYRRVVNLDPDHEG
                     ARAAIAGLASQWKSSIEDSLAAENLEQAGTKLEESLTVFPEDPSLVELTEQLTNRQHA
                     ESLLASTQALLRSHGLSDIPSATAAIQAYQEVLRLAPDHAVATEELNSLAQHYASLAA
                     EAADTGEVDAAISFLDRASAANSELRELDLVRSKIQQATTLQAAIIELLQQASAYRAA
                     GALINPPGENAAELYHRVLATYPENVIATQGLDEVVSQLRGTANQLLAAGDLGALQEL
                     VDRSSAVGLDPVAVNEMKSSLETEIARRATVAERLDQARGLLAEGFITEPQGRNVVAL
                     LREVERLDPGNEPAKRMLKESAERLASVAQEAYEVGMVDDAKHYLELALTVTPDVAAW
                     RELRATWEKSVASN"
     gene            2716..3849
                     /locus_tag="AC16VR_14185"
     CDS             2716..3849
                     /locus_tag="AC16VR_14185"
                     /inference="COORDINATES: protein motif:HMM:NF028007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR6012356.1"
                     /translation="MLQVTDLQYFISFHDDEFPLEDSLTIGRHLDNDVIVAGEDVLDY
                     HLRLEPTPRGPKVHPLGEASLILNGHHYSESRGLVAGDELVVGQNVLGVGVRRLHPAE
                     AESWSLHGDAAHGVHEMVGDLSVGRGEDSGLVVADDHVSREHALFTLADDVVWVRDLD
                     SANGTFVNGERVSGGCRLYHGDMIAFDQFEFQLIGQGADLTPVRAREGMQEGAMIKES
                     GPARSDTTEIAVVANADAVAELAPPAREKGTFLLGAAEPVLGITYRLRMGKTSLGRDD
                     ACDVVVKDRTVSSRHAEVVLRPEGATITNLMATNGTRVNGREIQSAQLADGDIIGLGR
                     VRFVYRSVTEGRRAARLRPLHRWLIAGAAIVAAVLAGLLIDTL"
     gene            3873..>4647
                     /locus_tag="AC16VR_14190"
     CDS             3873..>4647
                     /locus_tag="AC16VR_14190"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6012357.1"
                     /translation="MGVHIPYRRDLEFTYGEVAEIAPGIRRVIAENPSPFTLYGTGTY
                     ILGTGNVAVIDPGPADPAHIAAIVAGLQGETISHVLVTHTHMDHSPGCALLREHTDAP
                     TYAFGPHGAGKLEEGVPVEEGGDMEFVPDVVVRHGDVVEGADWSVECVYTPGHTSNHM
                     CFQLREQKALFTGDHVMGWSTSIISPPDGDMFAYMKSLDLLLERDDAVYWPTHGPCID
                     APKTHVRAFIEHRREREEQILKCVSRGVGQIRDMVPIMYV"
BASE COUNT          818 a         1488 c         1543 g          798 t
ORIGIN      
        1 cccgacgggc gtcgatacga gggcggcttt gctggtgatg cgaagtccgg caagggcgta
       61 ttggagtggc cgaacggaaa ccggttcgtc ggagatttcg cggaagatgc ccgttccgga
      121 ctggggatct tctactggcg tgacggcacc gtctaccagg gccagtacgc ccaggacaag
      181 atgcacggct acggcatcaa ggagcagcct gacggcgcca tggaactgca gcgctggcag
      241 gagggcgcgc ttcagttcgg caagcctctg ctggccgatg cgcgttgctc gctcaccatc
      301 gactcgcaac catggatgtt ctacagcacg cagtgcatca acgggctcgc tcacggccag
      361 ggtctggcgg tcagcctgga cgggcggctg atcgtcgagg agggcaggtt cgtgctcggc
      421 aacctgatcg agggggagat ccgggtcctc agactgggag agtcgtagtg acgttggagc
      481 ttcccggatt tcaggtgcag gcggagatcg gtcacggggc cacctcccga gtctatcttg
      541 ccatgcacaa gcagttgagc cgcctggtgg cgctcaagat cctcgcggag gaatacggta
      601 acgatcccgc cttccgcgtt cgctttctga acgagtcgcg catcggatcc agcctgtccc
      661 atccgaacat cgtccaggtt tacgatacgg gcgcccacga caacactctc tacctggtcc
      721 tcgaatacgt tcggggcggg aatcttctgc agcgcgtgga ccggggcatg cacatccgtg
      781 atctggttcg cgtggtgcag aacatcggca aagcgctgga ttacgcccat gcccgagggc
      841 tcgtccaccg ggacgtgaag ccggagaaca ttcttttccg cgaggacggc agcgccgtgc
      901 tcacagactt cggcatcgcc gggttcgtcg atccggacgg cccgccggcc aagggcggca
      961 ccgtaccggg gacgccgcag tacatgagcc ccgagcaggc cgccggcaag gcgctggatg
     1021 gccggtcgga tctgtacagc ctgggaatcg tttttttccg tgttctgacc ggggacgttc
     1081 cctacaaggc cgataccgcg gtgagtcttg gaatcaagca tctgcaggaa ccgatcccgc
     1141 ggctccccaa ccacctggcc atcttccagg acgtcgtcaa ccgcagcctg gcgaaaaagc
     1201 ccgaccagcg cttccagagc ggtcaggagt ttgccgacgc cctcgaagcc ctggcggcgt
     1261 cgggacaggt gcccagcctc atgatcaagg cccgtgccgt ggcgccggaa gagatccgct
     1321 ccctgggatc gaacgtcttt gcccgggccc gggactccgg gcggggcgag cgtcaatccg
     1381 cgccggagcg gcgccccctc accctaggca gagcggctct ggcggcgtcg ctggtagcgg
     1441 cggtcctcgc ctttggcatc gtcgtcgtcg agcagcctca gtggttcacc cgcatgcttg
     1501 ccgtcacggg catcgcagag gatcccgagg tggaagatgc ctggagcaac gcccagtctc
     1561 tgcaccagga tccgaatcag agcctggcga ccatcgttgc cggttaccgg cgggtggtga
     1621 atctggatcc cgaccacgag ggcgctcgcg ccgccattgc cgggctggcc agccagtgga
     1681 agagcagcat cgaggattct cttgcggcgg agaatctcga gcaggcgggt acgaagctcg
     1741 aagagtcgct gacggttttt cccgaggatc cctcgctcgt cgagctcacc gagcagctga
     1801 cgaaccggca gcacgcggaa agcctgctgg ccagcaccca ggctctgctg cgcagtcacg
     1861 ggctctccga cattccctct gcaacggctg ccatccaggc ctatcaggag gttctgcggc
     1921 tcgcgcccga ccacgccgtt gccacggagg agctcaactc ccttgcacag cactatgctt
     1981 cgctggccgc ggaagcggcg gatacgggcg aggtggatgc ggcgatcagc ttcctcgatc
     2041 gggcgtcagc cgccaactcg gagctgcgtg agctggacct ggttcgatcg aagattcagc
     2101 aggcgacgac gctccaggca gccatcatcg agctgctgca gcaggccagc gcctatcggg
     2161 cggccggcgc gctcatcaac cccccgggcg agaacgcggc cgagctctat catcgggtgc
     2221 tcgcaacgta ccccgagaac gtcatcgcca cacaggggct cgacgaggtg gtttcccagc
     2281 tgcgcgggac ggccaatcag ctgcttgccg cgggtgacct gggggcgctg caggagctcg
     2341 tcgaccgttc cagcgccgta ggcctcgacc cggtagccgt caacgagatg aagagcagcc
     2401 tcgaaacgga gatcgctcgg cgcgcgaccg tcgccgagcg gctcgatcaa gcgcgcgggc
     2461 tgctggcgga gggattcatc acggagcccc agggacgcaa cgtggtcgct ctgctgcgcg
     2521 aagtcgagcg tctcgatccg ggcaacgagc cggccaaacg catgctcaag gagtccgccg
     2581 agcgtcttgc cagcgtggcg caggaagctt acgaagtggg catggtggac gacgccaagc
     2641 actatctcga gctggcgctg acggtgacgc cggacgtcgc ggcctggcga gagcttcgag
     2701 cgacgtggga aaagagtgtt gcaagtaact gatctgcaat attttatttc ttttcatgac
     2761 gacgaatttc cgctcgaaga cagcctgacc atagggcgtc atctcgacaa cgacgtcatc
     2821 gttgccggtg aggacgtgct cgactaccac ctgcgcctgg aacccacccc acgggggccg
     2881 aaagtccacc cgctggggga ggcaagcctg attctcaacg gtcatcatta ctccgaatcc
     2941 cggggcctgg tggccggcga cgagctggtc gtcggccaga acgtgctcgg ggtaggggtt
     3001 aggcgcctgc atccggcgga agccgagagc tggtcgctcc acggtgatgc cgcacacggc
     3061 gttcacgaga tggtcggaga cctgagcgtg ggacgcggcg aggacagcgg tctggtcgtc
     3121 gccgacgacc acgtctcccg cgagcatgcg ctcttcacgc tggccgatga cgtggtgtgg
     3181 gttcgggatc tcgattcggc caacggcacc ttcgtcaacg gcgagcgcgt ctcgggaggc
     3241 tgccgtctgt atcacggcga catgatcgcg ttcgatcagt tcgagtttca gctcatcggg
     3301 cagggggcgg atctgacccc cgtgcgtgcg cgcgaaggca tgcaggaagg ggccatgatc
     3361 aaggaatccg ggccggctcg ctcggatacc accgagattg ccgtcgttgc caacgccgat
     3421 gccgtagccg agctggcgcc gcccgcccgt gaaaagggga cgtttctgct gggcgcggca
     3481 gagcccgtcc ttgggatcac ctaccgtctg cgcatgggca agacgtcgct cgggcgtgac
     3541 gatgcctgcg atgtcgtggt caaggatcgc acggtctcgt cgcgccacgc ggaggtcgtc
     3601 ctgcgtccgg agggcgccac catcaccaac ctgatggcga ccaacggaac ccgggtgaac
     3661 gggcgcgaga tccaaagcgc ccagctcgcc gacggcgaca tcatcgggct cgggagggtg
     3721 cgtttcgtct atcgcagcgt cacggagggc aggcgcgctg cgcgcctgcg tccgctgcac
     3781 cgttggctga tagcgggcgc cgccatcgtt gcggccgtcc tggcaggtct tctcatcgac
     3841 accctctagc gcgatggatt atgcttcgcc tcatgggcgt acacataccg tatcgacgcg
     3901 atctggagtt cacctacggt gaggtcgccg agatcgcgcc gggaatccgc cgcgtcatcg
     3961 ccgagaatcc cagcccgttc accctctacg gtaccggcac gtacattctg ggcaccggca
     4021 acgttgcggt gatcgatccc gggccggccg atccggccca catcgcggcc atcgtcgccg
     4081 gccttcaagg cgaaaccatt tctcacgtgc tggtgaccca cacccacatg gatcactccc
     4141 cgggctgcgc gctgctgcgt gagcacaccg atgcgccaac ttatgccttc gggcctcacg
     4201 gcgccggcaa gctcgaggag ggcgtccccg tggaggaggg cggcgacatg gagttcgtgc
     4261 ccgacgtggt ggtccgtcac ggtgacgtcg tcgagggcgc cgactggtcc gtggagtgcg
     4321 tctacacgcc tggccacacc tccaatcaca tgtgtttcca gctgcgcgag cagaaagctc
     4381 ttttcaccgg cgaccatgtc atgggctggt cgacgagcat catctcgccg ccggatggcg
     4441 acatgttcgc ctacatgaaa agcctcgatc tgctgctcga gcgcgatgac gccgtctact
     4501 ggcccaccca cgggccctgc atcgatgcgc cgaaaaccca cgttcgggcc ttcatagagc
     4561 atcgcaggga gcgggaggag cagattctga agtgcgtgag ccggggcgtc gggcagattc
     4621 gcgacatggt gcccatcatg tacgtcg
//
LOCUS       DBNHQR010000624        13773 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1200485, whole genome shotgun sequence.
ACCESSION   DBNHQR010000624 DBNHQR010000000
VERSION     DBNHQR010000624.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 13773)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13773)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13773
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1200485"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1278
                     /locus_tag="AC16VR_14195"
     CDS             <1..1278
                     /locus_tag="AC16VR_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011536293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HIR6012358.1"
                     /translation="MDEGSIAVLPAAGQRFRNRNTEYPFRQDSDFYYLTGFAEPDAVL
                     VLAPGRAHGQVILFCREREARAERYDGERVGPERAAERIGVDDAFPVNDLEEILPGML
                     EGRERIYITLGEYPEFDNRLMHWVAGIRAREAGGAIPPGEFVALKHLLHEQRLYKSAA
                     ELRAMREAARITCSAHRRAMAACRPGMTETQLEAELLHEFMVNGARSPAYPSIVGAGA
                     NACVLHYVDNHDVLKKGDLVLIDAGCEYQHYASDVTRTFPVAGKFSAAQRALYEVVLE
                     ANRQGIEACRPGATFIEPHSTALKVMVEGLIELNLIQGDVEEILQSERYREFCPHNSS
                     HWLGSDVHDVGDYRVEGAWRPLEPGMVLTVEPGIYIPADDSTRHLAPRWRGVGIRVED
                     DVAITRQGHEVLSASAPKSVDDIERLMRGGADG"
     gene            1271..2452
                     /locus_tag="AC16VR_14200"
     CDS             1271..2452
                     /locus_tag="AC16VR_14200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR6012359.1"
                     /translation="MADVVVVGGGLIGALAALGVADGGRRVTVLERREPERQRGRLGV
                     DIRNVAVSPASRELLEAVGVWASLEPAAYQRMEVWEERGTRGMHFDAAEVGRAELGWI
                     LESSQVVRSLWDLLQRHRNVRLEVGADLASLRLAASGAVLETDRGDIRARLVVAADGA
                     RSRVRELLAVPAEEAPTGHHALATVVRTEQEHEGTCLQRFLLEGPLALLPTADAHLSS
                     VVWSQPAEEAERRAKESDGDFCLELERCLEARFGKVEAVDARVVFPLAQTLVSTFNPH
                     HRVLLIGDAARVVHPLAGMGANIGFEDVRDLLAELAGIGGDQDPGAPGIWRRFARQRR
                     LRSQMVLALMSGFRRAYSADGPLASWVRNTGVGWLDRAQPLKAQIMREALGVGPLARR
                     W"
     gene            2541..3608
                     /gene="gcvT"
                     /locus_tag="AC16VR_14205"
     CDS             2541..3608
                     /gene="gcvT"
                     /locus_tag="AC16VR_14205"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016495293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HIR6012360.1"
                     /translation="MTRRTPLYELHVGLGAKMVDFAGWEMPLHYGSQLDEHHAVRRHV
                     GLFDVSHMTIVDVSGARSLDFLQRLVANDVARLEAPGQALYGVLLNPAGGVIDDLIVY
                     RRDADFRLVVNAATGEKVLNWLAGNNEEAVNILERDLAMLAIQGPAAVEMYQSLPDSL
                     EVRGLKAFTAAVRGEEMIARTGYTGEDGLEIMAPGDRARQLWLELIGSGAQPAGLGAR
                     DTLRLEAGLNLYGQDMDDETSPLVSNLAWTIAWEPSGRNFIGRQALEDERARGIAERL
                     TGIVLEDKGVIRRGCRVVTAAGDGHVTSGIFSPTLGYSIGLARVPKGAKGPCEVEMRG
                     RLKQARIVRPPFVRNGLQVFE"
     gene            3605..4000
                     /gene="gcvH"
                     /locus_tag="AC16VR_14210"
     CDS             3605..4000
                     /gene="gcvH"
                     /locus_tag="AC16VR_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014598064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HIR6012361.1"
                     /translation="MSDVPQELKYARTHEWARLEEDGTITVGISDHAQDALGDVVYVE
                     HPEPGQPVAAGDEAGVVESVKAASDIYAPVSGSVIAVNEALDDAPETVNQDPYGDGWF
                     FKIQPDDVAELDELLDSEAYAELCENEEH"
     gene            4032..5390
                     /gene="gcvPA"
                     /locus_tag="AC16VR_14215"
     CDS             4032..5390
                     /gene="gcvPA"
                     /locus_tag="AC16VR_14215"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HIR6012362.1"
                     /translation="MPFIPHTAEEVQVMLSQIGVARTEDLFDEIPQNLRAGALSSVPA
                     GASELEMLRKLGARAALDESVVCFAGAGSYDHHIPAAVWDLTSRGEFMTAYTPYQAEA
                     SQGTLQLIYEYQSMMTALTGLDVSNASVYDGASGLAEAVLMAVRSNKKARSRTVLLPD
                     SLHPHYAAAARNICRHQSVEFVGMALDHDGRVDLEALETDGPDEVCAVVVQQPNFFGM
                     LEDVDALTDWAAERGGLVIAVVNPLALAVLKPPGEWGGSGADIACGEGQPLGVPMSSG
                     GPAFGFICCRSALVRQLPGRVIGRTEDLTGKPGFTLTLQAREQHIRRGKATSNICTNQ
                     GLLVTAATIHMSLLGPLGLARIAEHCHANTRALVETLTAIDGVEARHQGPFFHERVLQ
                     LPRPADEVVAGLLERGILGGVALGAYHESMNHDLLVCATEKRTDDEIGRYAAALREVI
                     AR"
     gene            5387..6850
                     /gene="gcvPB"
                     /locus_tag="AC16VR_14220"
     CDS             5387..6850
                     /gene="gcvPB"
                     /locus_tag="AC16VR_14220"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HIR6012363.1"
                     /translation="MSDVRTIFELSAQNRGAAAQHLSERPGAMDDIPASLRRERAPGL
                     PEVSELQVVRHYTNLSRKNFSIDTNFYPLGSCTMKYNPRGAHRAASLPGFLNRHPLAP
                     DSASQGFMACLHELQEILKDVTGMQGVSLTPMAGAQGEFAGVAMIRAYHEARGDHARN
                     EILVPSAAHGTNPATAAMCGYRVAEVPVNASGDVDLEALKLAVGPQTAGLMMTNPSTC
                     GVFERGIESIAAVVHDAGGLLYYDGANLNAILGKVKPGSMGFDVLHMNLHKTFATPHG
                     GGGPGAGPVGVGERLLPYLPVPFVAQDAKGAYRWVTDAELPDSIGRLSAYAGNAGILL
                     RALAYALMLGREGMHRVGEYSTLNANYLARRLEDAGFELAYPDRRATHEFILTFSESA
                     RSRDVTAMDFAKRLLDYGIHSPTTYFPLLIPECFLIEPTETETKAELDAFVDALVAIQ
                     REAEADPEKVKGAPYDLPVRRLDDVKAARELDLAWRG"
     gene            complement(6847..8922)
                     /locus_tag="AC16VR_14225"
     CDS             complement(6847..8922)
                     /locus_tag="AC16VR_14225"
                     /inference="COORDINATES: protein motif:HMM:NF015168.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HIR6012364.1"
                     /translation="MSNRLADETSPYLRQHADNPVDWHPWDEAALALASEQQKPILLS
                     IGYSACHWCHVMAHESFEDPATARVMNEHFVNIKVDREERPDLDKVYQLAHQLLTQQG
                     GGWPLTMFLDPDTRLPFFGGTYFPKTPRYQLPGFVDLLLRIAETFASQRDELRDHGTR
                     VADVLGRLAGGDSQAGELGDAELLAAAREQLGAQADEDEGGFGGAPKFPMPETVTYLL
                     RHWAYAQRSGEADRQGLEFVMTTLTRMARGGIYDHLGGGFCRYATDRRWMIPHFEKML
                     YDNGQLLALYSDALAIGPDELFSQAVSDTADWLLREMRHPQGGFYAALDADSEGEEGK
                     FYLWRRDQVKRLLDEDEYLVVETLYGLDKPANFEGKWNLHRYDAWRSVVERLSMDRAA
                     ANDLLASARRKMLAARELRVRPGLDDKVLTAWNGLAIKGLARAGSRLGQSRWLDAAAQ
                     AADFARARLWRDGMLYATWQGAPRYPAYLDDYANLLDGLIALLGARWREEDAAFACKL
                     ADGALERFYDADRGGFFFTAHQHETLIHRPKPTMDDALPPGNGVMVQALTALGHLFGE
                     TRYLEAAAATLAWARAAMEQYPAGHCCLLSGLQDEIMPPELVVVRGAAATFPDWRRAA
                     VSGYTPWRAVYCIPFEGVSTLPAYLPGPVSDDPHSQTVAYVCSGLSCSLPIGKPEDLG
                     EALRSRPRV"
     gene            9064..9561
                     /locus_tag="AC16VR_14230"
     CDS             9064..9561
                     /locus_tag="AC16VR_14230"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HIR6012365.1"
                     /translation="MVDADGFRPNVGIVLSNSIGQVLWARRVGGQDAWQFPQGGIREN
                     ESVQAALFRELFEEVGLVADDVEVLASTRGWLRYRLPRRMRRYNSSPGFQGQKQKWFL
                     LRMLGDDARVQMDGSAKPEFDSWRWVSYWYPLDQVVSFKRDVYRAALKELAPHLPVED
                     VASPC"
     gene            9555..11840
                     /gene="ptsP"
                     /locus_tag="AC16VR_14235"
     CDS             9555..11840
                     /gene="ptsP"
                     /locus_tag="AC16VR_14235"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009396724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HIR6012366.1"
                     /translation="MLTTLRRIVHGVEESSSFKEALSLLVGEVREALGTEVCSAYLLN
                     PEGDSYVLVANEGLNTDAVDKLSLGLNQGLVTLVAERAEPINLSDATHHPRFYLVPEI
                     GEEAFNAFLGVPVIANRRVLGVLVVQERDARRFDESEEAFLVTLAAQLATVIAHHEAT
                     GEIQQLLEDSSVEPIAREDALYKGVPGAPGISLGTAVVMHPTASLEAVPERIAEDVRT
                     ELMVLDRAVNAVRDDIRRISEQFAQSLPPEELALFDAYLHMLDDNALAGDVRDRINRG
                     QWAQGALKQVIREHVRRFEAMEDPYLRERGADVRDLGMRVLAYLQDIRSRKTYFPDDT
                     ILVGHEITPSMLANIPPEKLKGIVSVRGSSNSHVAILARALGIPAVMGAMDLPIFSME
                     NISVVVDGFYGEVFTNPSLALAGQYLQLIAEEREFAEELEDLRELPSVTKDGWPVGLW
                     VNIGLTGDITRSLDRGAEGIGLFRTEVPFMEKERFPSEEEQRAIYREHMEAFDPRPVT
                     MRTLDVGGDKSLPYFPIHEDNPFLGWRGIRVTLDHPEIFLVQVRAMIKANAGLECLLR
                     IMLPMVSSLSEVEEAKALVERAYVEVIEEGIVVKQPLVGVMIEVPAAVYQARQLARMV
                     DFLAVGSNDLTQYMLAVDRNNPRVAQLYKEMHPAVLQALREVARAAHAEEKVVGICGE
                     LAGTPAGAVLLMAMGYDALSMNATNLPKVKWTLRNVKRSDARRMLVNVLHMDTAEEIQ
                     QFMQDWLIDSGLGRVVPSYHH"
     gene            complement(11849..12595)
                     /locus_tag="AC16VR_14240"
     CDS             complement(11849..12595)
                     /locus_tag="AC16VR_14240"
                     /inference="COORDINATES: protein motif:HMM:NF017551.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007030 - Golgi organization [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HIR6012367.1"
                     /translation="MCLVLIAYQPESPFPLVVAANRDELYARPARSAHYWADRPHLLA
                     GRDDSAGGTWLGVTRGGRFATVTNFAEEVAEDAPRSRGELPTAFLDSDVSAHDYAHHL
                     DGPSYRGFNLLVWDGDALVYTSNRGTTQDLAPGIYGLANAELGATWPKVTSGVGALRR
                     TLSSGPTVDDLLGLLQDDTQPPDSELPSRGRTIELERQVAPRFIRGDDYGTRASTAVI
                     IGADGIRFAEQEFGPQGVPGRRSDFEVARA"
     gene            12678..13511
                     /gene="lgt"
                     /locus_tag="AC16VR_14245"
     CDS             12678..13511
                     /gene="lgt"
                     /locus_tag="AC16VR_14245"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019485279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR6012368.1"
                     /translation="MHYPDIDPVIFAIGPLAIRWYGVAYLCAFAMCWWLGVVRAPAKG
                     WTREEVADIVFYGAVGAVLGGRVGYVLFYGFEELIRDPLFLIRIWDGGMSFHGGLLGT
                     LIALWWFGNRTHRSFLQVSDFVAPLVPVGLGFGRLGNFANTELPGRATDSVFGVIYPC
                     SAESIRSINPLCTGQWEAFARHPSPLYQAFAEGVVLFALVWLVSGRSSRTGVVSGTFL
                     IGYGVLRFVTEFFRQPDAHLQFEALEWLTMGQRLSIPMVIIGILLLFWPRLRGGGETR
                     G"
     gene            13618..>13773
                     /locus_tag="AC16VR_14250"
     CDS             13618..>13773
                     /locus_tag="AC16VR_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003110588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HIR6012369.1"
                     /translation="MKQYLDLMRQVRDTGVFKDDRTGTGTYSIFGHQMRFDLSESFPL
                     VTTKKVHL"
BASE COUNT         2292 a         4356 c         4751 g         2374 t
ORIGIN      
        1 atggacgaag ggtccatcgc cgttctgccg gcggccggcc agcgtttccg taaccgcaac
       61 acggagtatc cgttccgcca ggacagtgac ttctactatc tgacgggctt tgcggagccc
      121 gacgccgttc tggtgctggc gcccgggcgt gcgcacggtc aggtcatcct cttctgcaga
      181 gagcgcgagg cgcgcgccga gcgctacgac ggcgagcggg tagggcccga gcgcgccgcg
      241 gagcgcattg gcgtggacga cgctttcccc gtcaacgacc tggaggagat tctgcccggc
      301 atgctcgagg gacgggagcg gatctacatc accctgggcg aatacccgga gttcgacaac
      361 cgcctgatgc actgggtcgc cggcatccgc gcgcgggaag cgggcggggc cattccgccg
      421 ggtgagttcg tcgcgctcaa gcatctgctg cacgagcagc gtctctacaa atcggccgcc
      481 gagctcaggg cgatgcggga ggcggcccgt atcacctgct ccgcccatcg ccgcgccatg
      541 gcggcctgtc ggcccggaat gaccgagacg cagctggaag ccgagctgct gcacgagttc
      601 atggtcaacg gtgctcgcag cccggcctat ccgagcatcg tgggggccgg cgcgaacgcc
      661 tgcgttctgc actacgtgga caaccacgac gtgctgaaga agggcgatct cgttctcatc
      721 gacgccggct gcgagtatca gcactacgcc tcggacgtaa cccgcacgtt tcccgttgcc
      781 ggtaagtttt ctgctgccca gcgggctctc tacgaggtcg tcctcgaagc caatcgtcag
      841 ggcatcgagg cctgtcgccc cggtgcgacg ttcatcgagc ctcactctac ggcgctcaag
      901 gtcatggtgg aggggctcat cgagctgaat ctcatacaag gcgatgtgga ggagattctc
      961 cagagcgaac gctatcgtga attctgtccc cacaactcgt ctcactggct gggcagcgac
     1021 gtccacgacg tgggcgacta ccgggtcgaa ggtgcctggc ggccgctgga gccgggcatg
     1081 gtgctcaccg tggagcccgg catctacatc ccggccgacg actctacccg acacttggcg
     1141 ccgcggtggc gcggcgtggg cattcgtgtg gaagacgacg tggccatcac caggcagggc
     1201 catgaagtgc tttcggcgtc ggcccccaag tcggtggacg acatcgagcg gctgatgcgt
     1261 gggggcgccg atggctgacg tggtcgtggt gggcggtggg ctgatcggag cgctggccgc
     1321 cctcggcgtc gccgatgggg gacgacgggt cacggtgctc gaacggcgcg agccggaaag
     1381 gcagcggggc aggctgggcg tcgacatccg caacgtggcc gtatcgccag cgtcccgcga
     1441 gcttctggaa gccgtcggtg tgtgggcatc gctcgagccg gctgcttacc aacgcatgga
     1501 ggtatgggaa gagcgaggca ctcgcggcat gcacttcgac gcggcagagg taggccgggc
     1561 cgagctcggc tggatcctgg aatcgagcca ggtcgtgcgc tcgctctggg atctgctgca
     1621 gcggcaccgg aacgtccggc tggaggtcgg cgcggatctg gcgagcttgc gcctggcggc
     1681 ttcgggcgcc gtcttggaaa ccgaccgcgg agatatccgg gcgcgcctgg tggtggccgc
     1741 tgacggtgca cgttccaggg tgcgggagct gctcgcggtg cccgccgaag aagctcccac
     1801 cggtcatcat gccctcgcca cggtggtgcg caccgagcaa gagcatgagg gcacctgcct
     1861 gcagcgattc ctgctcgagg gcccgcttgc gctgctgccg acggcggacg cccacctttc
     1921 atccgtggtc tggtcgcagc ccgcggagga ggccgagcgg cgtgcgaaag agtccgacgg
     1981 cgacttctgc ctcgagctgg agcggtgtct ggaagcacga tttggaaagg tggaagcggt
     2041 ggatgcccgg gtcgtgtttc ccctggcgca gacgctggtg tcgaccttca atccccacca
     2101 cagggtgctg ctgattggcg acgccgccag ggtcgtgcac cccctcgcgg gcatgggcgc
     2161 gaacatcggc ttcgaggacg tgcgcgatct gctggccgaa ctcgccggga taggcggcga
     2221 tcaggacccg ggagcgccgg gaatctggcg gcgctttgcg cggcagcgac ggctacgatc
     2281 ccagatggtg ctggcgctga tgtccggctt ccggcgcgcc tattctgcgg acggcccgtt
     2341 ggccagctgg gttcggaaca cgggcgttgg atggctcgac cgtgcacagc ctttgaaagc
     2401 acagatcatg agagaggctt tgggggtcgg gccgctcgcc agaaggtggt gacgcccacc
     2461 gtttccgggg ccacccggtt cgcgtcatcc catgcgtcat cgcatacaat gtggtgcgtt
     2521 tttccaatgg tggtctgagc atgacgcggc gaaccccgct atatgagctg catgtcgggc
     2581 tgggcgcgaa gatggtcgac ttcgccggct gggaaatgcc gctccattac ggatcccagc
     2641 tggatgagca tcacgccgtg cgtcggcacg ttggcctgtt cgacgtctcc cacatgacca
     2701 tcgtggatgt cagcggtgcc cgcagtctcg acttcctcca gcggctggtg gccaacgacg
     2761 ttgccaggct ggaagcgccc ggtcaggcgc tgtacggggt gctgctcaat ccggcaggcg
     2821 gggtgatcga cgacctcatc gtctatcgcc gcgatgccga cttccggcta gtggtcaatg
     2881 ccgccactgg agaaaaggtg ctcaactggc tcgcgggcaa caacgaggaa gcggtcaaca
     2941 ttctggagcg ggacctggcc atgctggcca tccagggccc cgcggccgtg gagatgtatc
     3001 agtccctgcc tgacagcctc gaggtccggg gcttgaaggc gtttaccgcg gccgtgcgcg
     3061 gggaggagat gattgcccga acggggtaca cgggggagga cggtctggag atcatggccc
     3121 cgggcgatcg cgcccgtcag ctttggcttg agctcatcgg gtccggggct cagccagcgg
     3181 ggcttggggc tcgcgacact ctgcgcctgg aggctggcct gaacctctac ggccaggaca
     3241 tggacgatga aacctcgccc ctggtgtcca atctggcctg gaccatcgcc tgggagccat
     3301 ccggacggaa cttcatcggc cgccaggccc tcgaggacga acgcgcacgc gggatcgcgg
     3361 agaggctcac cggtatcgtg ctcgaggaca agggggtgat tcggcggggg tgccgcgtag
     3421 tgaccgcggc cggtgatggg cacgtcacca gtggtatatt ctccccgacg ttgggataca
     3481 gtattgggct cgcgcgggtg ccgaaaggcg cgaaggggcc gtgcgaagta gaaatgcggg
     3541 gaaggctgaa acaggcgcga atcgtccggc ctccctttgt gcgtaatggt ttgcaggtat
     3601 tcgaatgagc gacgttccac aggagcttaa atatgcgcgc acccacgagt gggcgcggct
     3661 ggaggaggac ggcaccatca ccgtcgggat ttccgaccac gcccaggatg cgcttggcga
     3721 tgtggtctac gtggagcatc cggagccggg tcagccggtt gcggccggcg acgaggcggg
     3781 cgtcgtcgag tcggtgaagg cggcctccga catctacgcg ccggtaagcg ggagcgtcat
     3841 tgccgtgaac gaggcgctgg acgacgcccc ggagaccgtg aaccaggatc cctacggtga
     3901 cggctggttc ttcaagatac agccggacga cgttgccgag ctggacgagc tgctcgattc
     3961 ggaagcctac gccgaactat gcgagaacga agagcactga gtccttgata cccatctaaa
     4021 cggagccccc gatgcccttc ataccgcaca ccgcggaaga agtgcaggtc atgctgtccc
     4081 agatcggcgt ggcccgcacg gaagacctgt tcgacgagat tcctcagaat ttgagagcag
     4141 gcgcgctgag ctcggtcccc gcgggggcca gcgagctgga gatgctgcgc aagctcggcg
     4201 cgcgcgcggc gctcgacgag tcggtagtct gcttcgccgg ggcgggcagt tacgaccatc
     4261 acattcccgc ggcggtctgg gatctgacga gccgcggcga gttcatgacg gcgtacacgc
     4321 cctatcaggc ggaagcgagc cagggcacgc tgcagctgat ctacgagtac cagtcgatga
     4381 tgaccgcgct cacgggtctg gacgtctcca acgcgtcggt ttacgacggc gcgtcgggcc
     4441 tggcggaggc cgtgctcatg gcggtgcgaa gcaacaagaa ggcccggtcg cgaaccgttc
     4501 tgctgccgga ctccctgcat ccgcactacg cggccgcggc ccgcaacatc tgccggcatc
     4561 agagcgtgga gttcgtcggc atggccctgg accacgacgg ccgcgtggat ctcgaggccc
     4621 tggagaccga tggtccggac gaggtctgcg cggtggtggt gcaacagccg aacttcttcg
     4681 ggatgctcga ggacgtcgac gccttaacgg attgggcggc cgagcggggg ggcctcgtga
     4741 tcgcggtggt caacccgctg gctctggctg tgctgaagcc tcccggtgag tgggggggca
     4801 gcggggccga catcgcctgc ggcgaaggcc agccgctcgg cgtgcccatg tcttcggggg
     4861 ggccggcgtt cggcttcatc tgttgccgct cggccctggt gcgtcagctt ccgggaaggg
     4921 tgatcggtcg gacggaggac ctgacgggga agccgggctt cactttgacc ctgcaggcca
     4981 gggagcagca catccgccga ggcaaggcca cgtctaacat ctgcacgaac cagggcttgc
     5041 tggtcacggc ggccaccatt cacatgagcc tcttggggcc gctcgggctc gccaggattg
     5101 ccgagcattg ccacgccaat acccgcgctc tggtggagac cctgaccgcc atcgacgggg
     5161 tggaggcgcg gcatcagggg ccgttctttc acgagcgggt gttgcagctg cccaggccgg
     5221 ccgacgaggt cgtagccggc ctgctcgagc gcgggatact cggcggcgtg gcgctcggcg
     5281 cgtatcacga gagcatgaat cacgacctgc tcgtctgcgc cacagaaaag cggacggacg
     5341 acgagatcgg ccgctatgcg gccgccctga gggaggtgat cgcccgatga gcgatgtgcg
     5401 aacgatattc gagctcagcg cgcagaaccg gggtgccgcc gctcagcact tgagcgagcg
     5461 gccgggggcc atggacgaca ttcccgcgag cctgcgtcgt gagcgggctc ccggcctgcc
     5521 cgaagtatcg gagcttcagg tggtgcggca ctacaccaac ctgtcgcgca agaatttctc
     5581 catcgatacc aatttctatc cgctcggctc atgcaccatg aagtacaacc cgcggggcgc
     5641 gcatcgcgct gccagcctgc cgggtttcct gaaccggcac ccgcttgccc cggactcggc
     5701 cagtcagggg ttcatggcct gcctgcacga gcttcaagag atcctcaagg acgtcacggg
     5761 catgcaggga gtgtccctca cccccatggc cggggcacaa ggcgaattcg ccggcgtggc
     5821 catgatccgc gcctatcacg aggcccgggg cgatcatgca agaaacgaaa tcctggtgcc
     5881 cagcgccgcc cacggcacca acccggcgac ggcggcgatg tgcggctacc gggtagcgga
     5941 agtgcccgtc aacgcctcgg gcgacgtcga cctcgaagcc ctcaagcttg cggtaggacc
     6001 ccagacggcg gggctgatga tgaccaaccc gtccacctgc ggggtctttg agcgtggcat
     6061 cgagagcatc gcggcggtgg tgcacgatgc cggcggcctg ctctactacg acggagccaa
     6121 cctgaacgcc attctcggca aggtgaagcc ggggtccatg ggcttcgacg tgcttcacat
     6181 gaacctgcac aagacgttcg cgacccccca cggcggcggc gggccgggcg cgggcccggt
     6241 gggcgtcggc gaacggctgc tgccctacct gccggtgccg ttcgtcgccc aggatgcaaa
     6301 aggcgcatac cggtgggtaa ccgacgccga gctgcccgac agcatcggcc gtctgtcggc
     6361 ctacgccggc aatgctggaa ttctgctgcg cgccctcgct tacgcgctga tgctcgggcg
     6421 ggaaggcatg caccgggtgg gcgagtactc cacgctcaat gccaactacc tggcgcgacg
     6481 tcttgaagat gcaggcttcg agctggccta tccggaccgg cgggcgaccc atgagttcat
     6541 cttgaccttt tcggagtcgg cacgcagccg ggacgtgacg gccatggatt tcgccaagcg
     6601 actcctcgat tacggcattc actctccgac cacctatttc ccgctgctga tacccgagtg
     6661 ctttctgatc gaacccaccg agacggagac caaggcggag ctggacgcgt tcgtggatgc
     6721 cctggtggcg attcagcgcg aagccgaagc cgatcccgaa aaagtcaaag gcgcgcctta
     6781 cgatctgccc gtcaggcggt tggatgacgt caaggccgct cgcgaactgg acctggcctg
     6841 gcgcggctag acacggggcc gggatctcaa cgcttcgccg agatcctccg gcttgccgat
     6901 gggcagcgag caggacagcc cgctgcacac gtatgccacc gtctggctgt gcggatcgtc
     6961 gctcacgggt ccgggaaggt aggcgggcag cgtgctgacg ccctcaaagg gtatgcagta
     7021 gacggcccgc catggcgtgt atcccgacac cgcggcccgg cgccagtcgg gaaacgtcgc
     7081 ggcggcaccg cggacgacga cgagctccgg gggcatgatc tcgtcttgca ggcccgagag
     7141 cagacagcag tggccggccg gatactgctc catggccgcg cgcgcccacg ccagagtggc
     7201 cgccgcagcc tcgaggtagc gcgtctctcc gaaaaggtgg cccagcgccg tgagcgcctg
     7261 aaccatcacg ccgttgcccg gcggcagcgc gtcgtccatg gtgggctttg gccgatgaat
     7321 cagggtctcg tgctggtggg cggtaaagaa aaagccgccc ctgtcggcat cgtagaaccg
     7381 ctccagggca ccgtcagcca gcttgcaggc gaaggcggcg tcctcttcgc gccaccgcgc
     7441 cccaagaagg gcaatcaggc cgtcgagcag gttggcgtag tcgtccaggt aggccggata
     7501 gcggggcgcc ccctgccagg tcgcgtagag catgccgtcc cgccagagcc gggcgcgcgc
     7561 gaaatcggcc gcctgagcgg ccgcatcgag ccatcgcgac tgccccaggc gtgacccggc
     7621 cctggccagg cccttgatgg ccaggccgtt ccaggcggtg agcaccttgt cgtcgaggcc
     7681 ggggcgaacc cgcagctccc gggctgccag catcttgcgg cgcgcgctgg ccagcaggtc
     7741 gttcgcagcg gcgcgatcca tggacagtcg ctcgaccacc gagcgccagg catcgtatcg
     7801 gtgcagattc cacttgccct cgaagttggc cggtttgtcc aggccgtaga gcgtttcgac
     7861 gaccagatac tcgtcctcgt cgagcagccg tttcacctgg tcgcgccgcc acaggtagaa
     7921 cttcccctcc tcgccctcgg agtccgcgtc cagcgcggcg tagaagccgc cctgggggtg
     7981 acgcatctcg cgcaggagcc agtcggcggt atcgctcacc gcctgggaga agagctcgtc
     8041 gggaccaatg gcaagcgcgt cgctgtagag agccaggagt tggccattgt cgtaaagcat
     8101 cttctcgaag tgaggaatca tccagcgacg gtccgtggcg tagcggcaga agccgccgcc
     8161 caggtggtcg tatatcccgc ctcgcgccat gcgcgtcagg gtggtcatga cgaactctag
     8221 cccctgccgg tccgcctcgc ccgacctctg ggcgtaggcc cagtggcgca gcaggtaggt
     8281 cacggtctcc ggcatgggaa atttgggtgc cccgccgaac ccgccctcat cctcgtcggc
     8341 ctgcgcgccg agctgctctc tggcagccgc cagcagctcg gcgtcaccga gctcgccggc
     8401 ctgtgaatct ccgcctgcca gccggccgag cacgtcggcg acccgcgtgc cgtgatctct
     8461 cagctcgtcg cgctggctgg caaacgtctc ggcgatcctc agcagaagat cgacgaatcc
     8521 gggcagctga tagcggggcg ttttcgggaa ataggtgccg ccgaagaatg gcagcctcgt
     8581 gtcggggtcc agaaacatgg tcagcggcca gccgccaccc tgttgggtga ggagctgatg
     8641 ggcgagctgg tagaccttgt ccagatcggg cctctcctcc cggtccacct tgatgttgac
     8701 gaagtgctcg ttcatgaccc gggcagtggc cgggtcctcg aaggattcgt gggccatcac
     8761 gtggcaccag tgacaggccg agtagccgat ggagagcagg atgggtttct gctgctcgct
     8821 ggccaacgcg agcgcggctt catcccaggg gtgccaatct accgggttat cggcgtgttg
     8881 ccgcaggtag gggcttgtct cgtcggccag gcggttgctc aaggttcgag gctcttttcg
     8941 gctgtgcagg gtcagcatcc tgacacggaa cacgaccggg tcaactcggt cggcccgtcg
     9001 gggccgcagt tggtaattcg gcccatattg aggttcaatc gagggaaatt cagaggaatg
     9061 ccgatggttg atgcggatgg tttccgccca aacgtcggaa tcgttctgtc gaacagcatt
     9121 ggccaggtgc tttgggcccg ccgggttggc ggccaggacg cctggcagtt tccgcaaggc
     9181 ggcatccgtg agaacgagtc agtacaagcc gcgctttttc gcgagctgtt cgaggaagtg
     9241 ggcctcgtgg cggacgacgt cgaggtgctg gcaagcaccc gcggctggct ccgctaccgg
     9301 ctgccgcgca ggatgcgtcg ttacaactcc agccccggtt tccaggggca gaagcagaag
     9361 tggtttctgc ttcgaatgct gggcgacgac gcgcgcgtgc agatggacgg gagcgccaag
     9421 cccgagttcg attcctggcg ttgggtgagc tactggtacc cgttggatca ggtcgtcagc
     9481 ttcaagcgtg acgtctaccg cgccgcgctc aaggagcttg cgccgcatct gcccgttgag
     9541 gatgtggcca gcccatgctg accacgctgc ggcggatcgt ccatggagtc gaggaatcca
     9601 gctcgttcaa ggaggcgctg agcctgctgg tgggcgaagt tcgagaggcg ctcggcaccg
     9661 aggtctgctc cgcttacctg ctcaaccccg aaggcgacag ctacgtgctg gtggccaacg
     9721 aggggctcaa taccgacgcg gtcgacaagc tttccctcgg cctgaaccag ggattggtga
     9781 cgctggtcgc ggaacgggcg gaacccatca acctcagcga tgccacccac catccccgct
     9841 tttacctggt gccggagatc ggcgaggagg cgttcaacgc gttcctcggc gtgcccgtca
     9901 ttgccaaccg ccgggtgctc ggggtgctgg tggtccagga gcgcgatgcg cgccggttcg
     9961 acgagagcga ggaggccttt ctggtcacgc tcgccgccca gctggcgacg gtgatcgctc
    10021 accacgaggc caccggcgag attcagcagc tcctggagga cagctcggtc gagcccatcg
    10081 cccgggagga cgctctttac aagggcgtcc ctggtgctcc gggaatcagt cttggcaccg
    10141 cggtcgtcat gcacccgacg gcgagcctgg aagcggtgcc cgaacgcatt gccgaggatg
    10201 ttcgcaccga gctcatggtg ctcgaccgag ccgtcaacgc ggtgcgcgac gacatccgga
    10261 ggataagcga gcagttcgct cagagcctgc ctcccgagga gcttgcgctc ttcgatgcct
    10321 acctccacat gctcgacgac aacgcgctcg ccggtgacgt gcgcgatcgc atcaatcgtg
    10381 gacaatgggc acaaggcgcc ctcaaacagg tgattcgcga gcacgtccgg cgcttcgaag
    10441 ccatggagga cccttatctg cgcgagcgcg gagccgatgt cagggatctg ggtatgcggg
    10501 tgctcgccta tcttcaggac atccgctcgc ggaagaccta tttccccgac gacaccatcc
    10561 tggtcggcca cgaaatcacc cccagcatgc tggccaacat cccaccggaa aagctcaagg
    10621 gcatcgtatc cgtgcgaggc tcgagcaact cccacgtggc gattctggcg cgggccctgg
    10681 gcatcccggc ggtcatgggg gccatggatc tgccgatctt cagcatggag aacatttccg
    10741 tcgtcgtaga cggtttctac ggcgaggttt tcaccaaccc ttcgctggcc ctggccggcc
    10801 agtatctcca gctcatcgcg gaagagcgcg agttcgccga ggagctcgag gacctgcggg
    10861 agctgcccag cgtcaccaag gacggctggc cggtggggct ttgggtcaac atcgggctca
    10921 ccggtgacat cacccgctct ctggaccgcg gcgcggaggg gatcggcctg ttccgcaccg
    10981 aggtgccctt catggagaag gagcgcttcc ccagcgagga ggagcagcgt gccatttacc
    11041 gggaacacat ggaggctttc gatccgcgcc ccgtgaccat gcgcacgctg gatgtgggtg
    11101 gggacaaatc gctgccctac ttccccatcc acgaggacaa ccctttcctc ggctggcgcg
    11161 gcatccgggt cacgctggac cacccggaaa tcttcctggt gcaggttcgc gccatgatca
    11221 aggcgaacgc cggcctggaa tgcctgctgc ggatcatgct gcccatggtc tcctcgctct
    11281 ccgaggtcga ggaagccaag gcgctggtgg agagggccta cgtggaggtc atcgaagagg
    11341 ggatcgtggt gaagcaaccc ctggtggggg tgatgatcga ggtgcccgcc gccgtatacc
    11401 aggcgcgcca gctggcccgt atggtggact tcctggcagt aggatcgaac gatctgaccc
    11461 agtacatgct tgccgtcgac cgcaacaacc cacgggtcgc ccagctttac aaggagatgc
    11521 atccggcggt tctgcaagcg cttcgggagg tggcccgggc ggctcacgcc gaagagaagg
    11581 tcgtgggcat ctgcggagag ctggcgggca ccccggccgg agcggtgctg ctgatggcga
    11641 tgggctacga cgcgctgtcc atgaacgcca ccaacctgcc gaaagtgaaa tggaccctgc
    11701 gcaacgtcaa acgctcagat gcccgtcgca tgctggtcaa cgtcctgcac atggataccg
    11761 ctgaggagat tcagcagttc atgcaggact ggctgatcga ctcgggcctg ggccgggtcg
    11821 ttcccagcta ccatcactga cacgcacctc acgcacgcgc gacctcgaag tccgagcgcc
    11881 tgccgggcac gccctgggga ccaaactcct gctcggcgaa gcggatgccg tccgcgccga
    11941 tgatgacggc cgtgctcgct ctggttccgt agtcatcacc ccggatgaat ctcggggcta
    12001 cctgtcgttc gagctcgatc gtgcgccccc ggctcggcag ctccgaatcc ggtggctggg
    12061 tatcgtcctg cagcaggcca agcaagtcgt cgaccgtcgg gcccgagctg agcgttcgcc
    12121 ggagcgcacc gaccccgctc gtcaccttcg gccaggtcgc cccgagctcc gcgttcgcca
    12181 ggccgtagat gccaggcgcc agatcctggg tggtgccccg gttggaggtg tagaccaggg
    12241 cgtccccgtc ccagacgagc aggttgaagc cgcggtagct gggcccgtcg aggtggtgtg
    12301 cgtagtcgtg tgccgagacg tcgctgtcaa gaaaggcggt cggcagctcg ccgcgggagc
    12361 ggggcgcgtc ctcggctact tcctcggcga agttggtgac cgtggcgaag cggccgcctc
    12421 gtgtgacgcc gagccaggtg ccgccggcgc tatcgtcccg gccggccaga agatggggcc
    12481 tgtcggccca ataatgtgcg ctgcgcgcgg gtcgcgcgta tagttcgtca cggttggccg
    12541 ctacgaccag gggaaagggc gactcgggct ggtaggcgat taagacgaga cacatgctct
    12601 ttcgcgcggc tggtggtcgg aatggaacgg cgtggcaaga aatttcagtg taacagaccc
    12661 ggcctgcgga ggttgtcctg cactatcctg acatcgaccc cgtcatcttc gccatcgggc
    12721 ccctggccat ccgctggtac ggggtggctt acctctgcgc ctttgccatg tgctggtggc
    12781 tcggggtcgt gcgggcgccg gcgaaggggt ggactcggga ggaggtggcc gacatcgtct
    12841 tttacggcgc ggtgggcgcc gtgctggggg gccgcgtcgg gtacgtgctg ttctacgggt
    12901 tcgaggagct gatccgggac ccgctctttt tgatccgcat ctgggacggc gggatgtcgt
    12961 tccacggcgg gctgctgggc accctgatcg cgctgtggtg gttcgggaac cggacgcacc
    13021 ggagcttcct gcaggtcagc gacttcgtgg cgccgctggt gcccgtcggt ctgggattcg
    13081 gacgtctggg caacttcgcc aacaccgagc tgcccggtcg cgccacggac tccgtcttcg
    13141 gcgtgatcta tccctgctcg gcggagtcca tccgcagcat caacccgctg tgcacaggac
    13201 agtgggaggc ctttgcacgc cacccttcgc cgctttacca ggcctttgcc gagggcgtcg
    13261 tgctgttcgc tctggtgtgg ttggtctccg ggcggtccag caggacgggg gtggtgtcgg
    13321 gcacctttct gatcggctac ggggttttga ggttcgtcac cgagttcttc cgccagccgg
    13381 atgcccacct ccagttcgaa gcgctggagt ggctgaccat gggccagcgg ctctcgatac
    13441 cgatggtcat cattggtatt ctgcttctgt tctggccccg cttgcgtgga gggggcgaaa
    13501 cgcggggctg acaagcgagc gccaacagcc ggcactggcc ccgatcgcca cagcgggtcc
    13561 ggtaatcacg gggccgcgag cgcggccgaa ctcgggacga gcagccaacg gaagccagtg
    13621 aaacagtatc tcgatttgat gcgccaagtg cgcgatacgg gcgtcttcaa ggacgaccgc
    13681 accgggacgg gaacgtacag catcttcggt caccagatgc gcttcgatct gagcgagagc
    13741 tttccgctgg tgacgacgaa gaaggtccac ctg
//
LOCUS       DBNHQR010000625         2354 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1454058, whole genome shotgun sequence.
ACCESSION   DBNHQR010000625 DBNHQR010000000
VERSION     DBNHQR010000625.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1454058"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="AC16VR_14255"
     CDS             <1..384
                     /locus_tag="AC16VR_14255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HIR6012370.1"
                     /translation="AGAVRESYGRTDLPDMLVPVPTAPGRLAWRGHNQAATIAAFLSR
                     RLSLPLARRGARRARSSRPQRLLSRAARLKATAGIFESRQWRGETIAVVDDVMTTGAT
                     VAELARTLTAAGAGEVHVWSATRAV"
     gene            407..1393
                     /locus_tag="AC16VR_14260"
     CDS             407..1393
                     /locus_tag="AC16VR_14260"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HIR6012371.1"
                     /translation="MSESSTSTPYAGLTPDLILDALEATGFAPTGSLLELNSYENRVY
                     QLEVEDGTFVVTKFYRPERWSDAQILEEHAFTAELIEHELPAVGPLRRDGTTLFEHSG
                     FRFAVFPRQGGHPPNIEVEEDLKVLSRTLARLHAIGRIRPFETRPTLSCERMGRQSRE
                     FLLETNLVPLELQPAYASTTEHLLERLEPLFDTEHQWRRIHGDCHLGNLLWRGEVPHF
                     VDFDDCVNGPPIQDLWMLLSGEHHEREAQLSMILEAYDQFASFDLSSLRLIEPLRTLR
                     IMHHAAWIGRRWSDPAFPRAFPWFNTEKYWSEHILSLREQMAALDEPTLTVS"
     gene            complement(1374..>2354)
                     /locus_tag="AC16VR_14265"
     CDS             complement(1374..>2354)
                     /locus_tag="AC16VR_14265"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR6012372.1"
                     /translation="RAYLRRKTKGLVTVNDVQLFLGKYMARLIEETVVELTVDGLDEL
                     SKGKPYLFMSNHRDIVMDSGILNYVIHRAGHQTSRIAVGDNLFARPFAADLMRLNKSF
                     VVERKVSGTRAVYAALSRTSHYIRQSLEEGESVWIAQREGRSKDGFDRTDPALLKMLG
                     LAYRKEAGSFGDLVTRIRIVPVAVSYELDPCDLRKAHELYLTEREGSYIKPPDEDFQS
                     IVEGMMGFKGRVHLHFGSPVTGYFETAEALALEIDRAIVAGLKIFPTQAQAAARLGQA
                     SCHVQLAELPAVTAAFEQRLAGCPDAEKPYLLAAYANVLRNRRELSLPSA"
BASE COUNT          409 a          787 c          754 g          404 t
ORIGIN      
        1 gccggggcgg tccgtgaaag ctacggcagg accgaccttc cggacatgct ggtgcccgtt
       61 cccacggctc cgggacggct ggcctggcgg ggtcacaatc aggcagccac catcgccgct
      121 ttcctgagcc gccgcctgag cctgccgctg gcccggcgcg gagccaggcg cgcccgcagc
      181 tcccggcccc agagacttct gtcccgcgcc gcacgcctga aggccactgc cggcatcttc
      241 gaatcacgtc agtggcgggg cgagacgatc gccgtggtcg acgacgtcat gaccaccggg
      301 gctacagtcg ccgagctcgc acgcacgctg acggcggcag gtgcgggcga agtccacgtg
      361 tggtcggcca cccgcgccgt ctgagttccc tggtagactg gctctcatga gcgagtcgtc
      421 caccagcaca ccctatgccg gcctgacccc ggacctgata ctcgacgcgt tggaggccac
      481 cgggttcgcc ccaaccggaa gcctgctcga gctgaacagc tacgaaaacc gggtctatca
      541 gctggaggtt gaggacggga cgttcgtcgt gaccaagttc taccgtcccg agcgctggag
      601 cgacgcgcag atcctcgaag agcacgcgtt caccgcagag ctcatcgagc atgagctgcc
      661 cgcggtgggg ccgctgcgga gagacggcac caccctgttc gagcactccg gattccgttt
      721 cgccgtgttc ccgaggcagg gcgggcaccc tcccaacata gaggtcgagg aagatctgaa
      781 ggttctctcc cgcacgctgg cccggcttca tgccatcggt cggatccgcc cgttcgagac
      841 acgccccacg ctcagctgcg agcgaatggg caggcagagc cgcgagtttc tgctggaaac
      901 gaacctcgtt cccctggagc ttcagccggc ctacgccagc accaccgagc atctgctcga
      961 gcggctggag ccgctgttcg ataccgagca ccaatggcgc cgcatccacg gcgactgcca
     1021 cctgggcaac ctgctgtggc gcggcgaggt tccacacttc gtcgatttcg acgactgcgt
     1081 gaacggtccg ccgatacagg acctgtggat gctgctgtcc ggcgagcacc acgagcgcga
     1141 agcccagctg agcatgatcc tggaggctta cgatcagttt gcgagcttcg acctgtcgag
     1201 cctcaggctg atcgaacccc tgcgcacgct gcgaatcatg caccacgctg cctggatcgg
     1261 ccgccgctgg agcgatccgg cctttccccg tgcgttcccg tggttcaaca cggagaagta
     1321 ctggtcggaa cacattctca gcctgcgaga gcagatggcc gctctcgacg agcctacgct
     1381 gacggtaagc tgagctcccg gcgattgcgc aggacgttgg cgtacgccgc caggagatag
     1441 ggcttctcgg cgtcggggca cccagcaaga cgctgctcga aggcggcggt cacggcagga
     1501 agctcggcca gctgcacatg gcagctggcc tggccgagcc gggccgccgc ctgcgcctgg
     1561 gtgggaaaga tcttcaggcc cgcgacgatg gcgcgatcga tctcgagggc gagggcttcc
     1621 gcagtttcga agtagccggt cacgggcgag ccgaagtgca gatgcacgcg acccttgaag
     1681 cccatcatgc cctccacgat gctctggaaa tcctcgtccg gcggcttgat gtaggagcct
     1741 tccctttccg tcaggtaaag ctcgtgggct ttgcgcagat cgcagggatc gagctcgtag
     1801 gagacggcta ccgggacgat gcggattcgc gtgacgagat ccccgaagct ccccgcttcc
     1861 ttgcggtagg cgagacccag catcttcagc agggccggat cggtgcgatc gaaaccgtcc
     1921 ttcgatcgac cctcgcgttg cgcgatccac accgactcgc cctcctcgag cgactggcgg
     1981 atgtagtggg acgttcgcga aagcgcggcg tagacggctc gggtaccgct gactttgcgc
     2041 tcgaccacga agcttttgtt gaggcgcatc aggtcggcgg cgaacggccg cgcgaacagg
     2101 ttgtccccga ccgcgatgcg cgacgtctgg tgccccgctc gatgaatcac gtagttgagg
     2161 atgcccgagt ccatcacgat gtcccgatga ttcgacatga acaggtaggg cttgccctta
     2221 gacagctcgt ccaggccgtc caccgtcagc tccacgacgg tctcctcgat caggcgggcc
     2281 atgtactttc ccaggaacag ctgaacgtcg ttgacggtga ccagcccctt cgtcttgcgt
     2341 ctgaggtagg ctct
//
LOCUS       DBNHQR010000626         6581 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_568629, whole genome shotgun sequence.
ACCESSION   DBNHQR010000626 DBNHQR010000000
VERSION     DBNHQR010000626.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6581)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6581)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6581
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_568629"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="AC16VR_14270"
     CDS             <1..426
                     /locus_tag="AC16VR_14270"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HIR6012373.1"
                     /translation="RLMIDLPMEEWRAGWNAVFAGAFQDVQARLPTVRQSMPVYLFTN
                     TNATHQAAWSHLYADTLSHFDALYVSSEIGHRKPNVSAFRHVAEDMGYAPEQIVFVDD
                     TKENVLGARAAGMDARMVGSEADVVRILDELTGTEPGDS"
     gene            complement(421..1293)
                     /locus_tag="AC16VR_14275"
     CDS             complement(421..1293)
                     /locus_tag="AC16VR_14275"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6012374.1"
                     /translation="MTDFGVLIFPTAYSISPSDLARAAEARGFESIFFPEHTHIPACR
                     TTPWPGGRELPREYWNTYDPYVGLAMAAAVTKTLKVGTGITLVTERDPIVLAKQVASL
                     DRLSGGRVLLGIGAGWNVEEMRHHGVAFDERWAVLRERVLAMRAIWTEQAPEFHGEHV
                     DFEPLWSEPKPLQAGGPKILMGASSRWTWARIAEYADGWFPIHQDPARASAQGAVDYP
                     QGIQATREAWQAAGRQGEPDFSIFGMGPDRGRTEEMIRLGFNRVIFGLPSADADTVLP
                     LLDRYAEIGHAVNS"
     gene            complement(1309..2889)
                     /locus_tag="AC16VR_14280"
     CDS             complement(1309..2889)
                     /locus_tag="AC16VR_14280"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR6012375.1"
                     /translation="MLEVPVLIVGGGPVGLAAALECAYFGVPAMLVERHSGTTWHPKA
                     RNLNSRTMEIMRRLGLEPELQAVGLPEAWTRQIVYADTLSGAELGRMGTGGFESRSSA
                     ASPCAAVLSSQDVFEPIWRGRAEQQPLLDVRYGHQAEILDVDAAGAVVRLTGARGGDV
                     RARYLLAADGAGGAIRQGLGIGLEGPENLGHFINVYYRANLDRVVAHRPAVLYFTANE
                     AGRGVFQPLDGRKRWLSQIAYDGAADTRAGFDGERCAQWIRNAAGEPDLKVEILGISH
                     WTMNATVAERYRQGAAFLVGDAAHQLPPTGGFGLNTGIQDVHNLVWKLAYDLRDWSDA
                     GLVDTYEAERKPVARFNADRAMDNSRAVARIQRAVADGEAAAGEVARSRRYGNFRGLE
                     LGYSYRSAAVIDDGSPAPEPEDAVEDYVPCARPGHRMPHMWLEGGALSTLDLVQRRFL
                     VLSDSARWLEAASQHNADCRVLPGNGGFLQLFGLEPGGAVLVRPDGHVAARFAEPPGF
                     PGGALASALRDLLNTTTT"
     gene            complement(2958..3611)
                     /locus_tag="AC16VR_14285"
     CDS             complement(2958..3611)
                     /locus_tag="AC16VR_14285"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR6012376.1"
                     /translation="MPTVATARQNENRKEAKRAKVRWYTPFLFVLHSLAIVGPVLLAA
                     GFAHRVEDAAYRTFAFAVPAPENSYVTWNSEVSEFGTKVSQAFGVRPSTATEFAGWIL
                     EASTRQELEPELLASLVLTESSFRKNVRSHVGAIGPAQVRPEYWSNFCGIANLHDPAE
                     NIYCGAQVLSHLRDRCGDEGCALHAYNVGLYAPRHKAGHRYVAKIDHYREQLLNLPL"
     gene            complement(3869..4876)
                     /locus_tag="AC16VR_14290"
     CDS             complement(3869..4876)
                     /locus_tag="AC16VR_14290"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR6012377.1"
                     /translation="MKVDGGVGWELDLVGAQAQELEEMGYSGILSAETSHDPFFPLLV
                     AAQNTERVDLMTSIAVAFARSPMTLANIGHDLNAVSKGRFVLGLGSQIRAHIMKRFSM
                     PWSHPAARMREFILAMRAIWSNWHEGEPLAFTGKFYTHTLMTPFFTPTDTEYGPPRVF
                     LAAVGPMMTEVAGEVADGVIVHAFTTEKYLRETTLPALERGFAKAGKTRADFEISYPV
                     FVVTGENEEELAQAKVATARQIAFYGSTPAYKPVLESIGVGEMQGELNAMSKQGRWEE
                     MGHLISDDVLEAFAVVGEPQQIAGQIISRYGDIVDRTSAAYAHISKEHRQEIIAELRL
                     A"
     gene            complement(4895..5572)
                     /locus_tag="AC16VR_14295"
     CDS             complement(4895..5572)
                     /locus_tag="AC16VR_14295"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR6012378.1"
                     /translation="MKRLYLIRHGQTEWNVAKRMQGRLDSPLTSAGRIQAAVHGRLLS
                     EIASLEQLVVSPSGRTRETACILNSHVGAPMRHDEALMERDCGEWSGLTIEEIEQHFP
                     EAWEARREDPFYHRPPGGENHEDLVQRAGPLLAEFLAGDVTEVGLVTHGVMSRAIVTH
                     LLELTPVEAVGIRHPNDLVYRLDICATGVQVSHFLAGDGPRAGLLRQNENETISGPAR
                     AGERRDR"
     gene            complement(5606..6448)
                     /locus_tag="AC16VR_14300"
     CDS             complement(5606..6448)
                     /locus_tag="AC16VR_14300"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6012379.1"
                     /translation="MEYQKIIVDEPRPMVRRITLNRPEKRNPLSNELRSEMFHALESA
                     DQDDGVRVTIIRGAGPCFSAGYDLKSDTSQNPPFYTAGGLGHWPRHVVEGFFRMWDMA
                     KPIIAQVHGYCLAGGTELATACDLVYVAEDAKIGYPVVRTMSPPDNQFYPWIVGLRRA
                     MELMLTGDHMSGVEAAESGFANRAFPAEELEERVLAIAERVALTPTDLQQFNKRAVHR
                     QMDAMGIRAGIRAGTEMQQLAFFTDTTRAYFKEMSKGLTHALSKRDEAFGDYRTSASG
                     KAED"
BASE COUNT         1134 a         2223 c         2062 g         1162 t
ORIGIN      
        1 cggctcatga tcgacctgcc catggaagag tggcgcgccg gctggaacgc cgtcttcgcc
       61 ggcgcttttc aggacgtcca ggcgcgtctg cccaccgtgc ggcagagcat gcccgtgtac
      121 ctgttcacca acaccaacgc cacgcaccag gctgcctgga gccatcttta tgcagacacc
      181 ctgagccact tcgacgcgct ctacgtctcg tcggagatcg gccaccgcaa gccgaacgtc
      241 tctgcgttcc gccacgtggc cgaggacatg ggctacgcgc ccgagcagat cgtcttcgta
      301 gacgacacca aggagaacgt gctcggcgcc cgggccgcgg gcatggacgc gcgcatggtc
      361 ggcagcgaag ccgacgtcgt gcgcattctc gacgagctca ccggcacgga gcccggggat
      421 tcatgagttg acggcgtgcc cgatctcggc gtagcggtcg agcagcggca acaccgtatc
      481 cgcgtccgcc gagggcaacc cgaatatcac ccggttgaaa cccagccgga tcatctcttc
      541 ggtgcgcccc cggtcaggcc ccattccgaa gatgctgaag tcgggctccc cctgccggcc
      601 ggctgcctgc cacgcctcgc gggtggcctg tatcccctgc gggtagtcca cggctccctg
      661 ggccgacgcc cgggcgggat cctggtgaat ggggaaccag ccgtccgcgt actctgcgat
      721 ccgcgcccac gtccaccggg aggaagcgcc catcaggatc ttcgggccac ccgcctgcag
      781 cggcttcggc tcggaccaca gaggctcgaa gtcgacgtgc tccccatgaa actccggcgc
      841 ctgctccgtc catatggccc gcatggcaag cacccgctcc ctgagcacgg cccaacgctc
      901 gtcgaaagcg accccgtgat gacgcatctc ctcaacgttc caaccggcac cgatgccgag
      961 cagcacccgg cccccggaca gacgatccag agaagccacc tgcttggcga gcacgatggg
     1021 atcccgttcg gtaaccagcg tgatccccgt acccaccttc aacgtcttcg tgaccgctgc
     1081 ggccatggcg agccccacat aggggtcgta ggtattccag tactcgcgcg gcagctccct
     1141 gcctcccggc cagggcgtgg tccggcaggc cggtatgtgg gtatgctccg gaaagaagat
     1201 cgactcgaaa ccgcgggcct cggccgcgcg cgccaggtcc gaaggggaga tggagtaggc
     1261 ggtggggaag atgagcacgc cgaagtccgt catctggcaa tctccttatc aggttgtggt
     1321 ggtgttgagc agatctctga gcgccgacgc cagcgccccg ccgggaaagc cgggaggctc
     1381 ggcgaatcgg gccgccacgt ggccgtccgg gcgcaccagc accgcgccac ccggctcgag
     1441 cccgaacagc tggagaaaac cgccattgcc ggggagcacc cgacagtcgg cgttgtgctg
     1501 agaggccgct tccagccagc gcgccgagtc cgaaagcacc agaaagcgcc gttgcaccag
     1561 gtccagggtg gacagggcgc ctccctccag ccacatgtgg ggcatgcggt gaccggggcg
     1621 ggcacagggc acgtagtcct ccacggcatc ctccggctcg ggcgccgggc tgccgtcgtc
     1681 gatcaccgct gccgatcggt agctgtaccc cagctccaga ccccggaaat tgccatagcg
     1741 ccgcgaccgc gccacctcgc ccgccgcggc ctcaccgtcg gcgaccgccc gctggatgcg
     1801 tgccacggcg cgcgagttgt ccatggcccg atccgcgttg aaccgggcca ccggctttcg
     1861 ctccgcttcg taggtgtcga cgagaccggc gtccgaccag tccctcaagt cgtaggccag
     1921 tttccatacg aggttgtgca cgtcctgaat gcccgtgttc aggccgaatc cgccggtggg
     1981 cggcagctgg tgcgcggcgt ctccgacgag aaacgccgcg ccctgccggt atcgttcggc
     2041 caccgtcgcg ttcatggtcc agtgagatat gccaaggatc tcgaccttga ggtcgggttc
     2101 ccccgcggcg ttcctaatcc attgtgcgca tcgctcgcca tcgaacccgg cgcgagtgtc
     2161 cgccgcgccg tcgtaggcaa tctgcgacag ccagcgcttg cgcccgtcca ggggctgaaa
     2221 cacgccgcgc cccgcttcgt tggccgtgaa gtaaagcacc gccgggcgat gagccaccac
     2281 tcggtcgaga ttggctcgat agtagacgtt gatgaagtgg ccgagattct cgggcccttc
     2341 cagcccgatg cccagccctt gccggatcgc accaccggcc ccgtccgccg ccaggaggta
     2401 gcgggcgcga acatcgcccc ctctggctcc ggtgaggcgc acgacggctc ctgcggcgtc
     2461 cacgtcgagg atttccgcct gatgcccgta gcgcacgtcc aggagcggtt gctgctccgc
     2521 ccgccctcgc catatgggct cgaacacgtc ctgtgagctc aaaaccgccg cgcacgggct
     2581 ggctgccgag cttcggctct cgaacccccc ggtgcccatg cgtccgagct cggcaccgct
     2641 gagcgtatcg gcatagacga tctggcgcgt ccaggcctcg ggaaggccaa cggcctgcaa
     2701 ctcgggctcc aagccaagcc gacgcatgat ctccatggtc cgggagttga ggttacgcgc
     2761 cttgggatgc caggtggtgc cggaatgccg ctcgacgagc atcgccggca cgccgaaata
     2821 ggcgcactcg agcgctgcgg ccagccccac ggggccacct ccgacaatga gaacgggaac
     2881 ttcgagcaac gcgcatcccc tggggcgcca gccaagaggg tacgcccttc cgggcgggct
     2941 aggctagagg cgtcagatca aagaggcagg ttgaggagct gctcgcgata gtggtcgatc
     3001 ttggcgacgt agcggtgtcc ggccttgtgc cgcggggcgt agaggccaac gttgtaggca
     3061 tgcagcgcgc acccttcgtc accgcagcgg tcccgcagat gggaaagcac ctgggcacca
     3121 cagtagatgt tctccgcggg gtcgtggagg ttggcaatgc cgcagaagtt gctccagtac
     3181 tccggccgca cctgggccgg gccgatggcc cccacgtggg agcggacgtt ctttcgaaac
     3241 gagctctcgg tcagcaccag gctggccagc aattcaggct cgagttcctg ccgtgtcgat
     3301 gcctccagga tccaccctgc aaactccgtg gccgtgctcg gccgcacgcc aaacgcctgg
     3361 gacaccttgg tgccgaactc gctcacctcg gagttccagg tcacgtagga gttttccggc
     3421 gctggcaccg caaacgcgaa agtccggtag gcggcgtcct cgacgcggtg cgcaaagcct
     3481 gcggccagca gcaccgggcc aacgatcgcc aggctgtgca gcacgaagag gaagggcgtg
     3541 taccagcgca cttttgcgcg ctttgcctcc ttccggttct cgttctgcct tgccgttgcc
     3601 accgttggca cctcgtctcg tgttcatatc gactgtgtac ggctgcgcat tctaaagacc
     3661 tgaaatgcca caatgaaccc aaatttcgtg cgaagtcgtt ggcaggttgc caccgaaacg
     3721 ttgggagaat gcaaaaatgc gggccgtgca cggacttttg tgaggaattc cttaccgtcc
     3781 acgccccgtc acgcttgaat cgcgaatcag aacaccgctc gattactttt tatacctaaa
     3841 agtaatcaat ttttcatttt ttagaacatt atgccaatct cagctcggcg atgatctcct
     3901 gacggtgttc tttggaaatg tgcgcatagg cagccgaggt gcgatcgacg atgtcgccgt
     3961 agcgagagat gatctgaccg gcgatctgct gcggctcgcc caccacggcg aacgcctcga
     4021 gcacgtcgtc gctgatgagg tgccccatct cttcccaccg gccctgcttg gacatggcgt
     4081 tcagctcgcc ctgcatctcg ccgacgccga tgctttccag caccggcttg taggcgggcg
     4141 tggacccata gaaggcgatc tgacgcgccg tggccacctt cgcttgcgcc agctcttcct
     4201 cgttctcccc ggtcacgacg aataccggat agctgatctc gaagtcggcg cgcgtcttgc
     4261 ccgccttggc aaaaccccgc tccagagccg gcagcgtggt ttcgcgcagg tacttctccg
     4321 tggtgaacgc gtgaactatc acgccgtcgg cgacctcgcc ggcgacctcg gtcatcatgg
     4381 gcccgacggc ggcgagaaag acgcgcggcg gtccgtattc ggtgtccgtg ggggtgaaga
     4441 acggcgtcat gagcgtgtgg gtgtagaact tgcccgtgaa ggccaggggc tctccctcgt
     4501 gccagttcga ccagatggcc ctcatggcca ggatgaattc gcgcatgcgc gccgccgggt
     4561 gagaccaggg catgctgaat cgcttcatga tgtgcgcccg gatctgcgag ccgaggccca
     4621 gcacgaaacg gcctttggaa acggcgttca gatcgtggcc gatgttggcc agagtcatgg
     4681 gcgatcgggc aaaagccacc gcaatcgagg tcatcaggtc gacccgctcc gtgttctgcg
     4741 ccgccacgag cagcgggaag aacgggtcgt ggctggtttc agccgacagg attcccgaat
     4801 agcccatctc ctcgagctcc tgggcctgag cgcccaccag atcgagttcc caacccacac
     4861 cgccgtcgac tttcatcgtc ttcccccagt ttttttatcg gtcccgccgc tctccggccc
     4921 gagcggggcc ggagattgtt tcattctcgt tttggcgaag caatcccgcc cgcggaccgt
     4981 ctcccgcgag aaagtgactg acctggacac cggttgcgca gatatccagc cgatagacca
     5041 gatcgttggg gtgacggatc ccgaccgctt ccacgggcgt cagctcgagc agatgggtca
     5101 cgatcgcccg ggacatgacg ccgtgggtca ccaggcccac ctcggtaacg tctccggcca
     5161 gaaactccgc cagcaacggg cccgcccgct gcaccagatc ctcgtgattc tcgccgccag
     5221 gtggccggtg ataaaaggga tcctcccgcc gggcctccca ggcctccgga aagtgttgct
     5281 cgatctcttc gatggtgagc cccgaccact caccgcagtc gcgttccatg agggcttcgt
     5341 catgacgcat cggcgcccca acgtgggagt tcagtatgca ggcggtttcg cgcgtccgtc
     5401 cggacgggga caccaccagc tgctccagac tggcgatctc gctcagcaga cgaccgtgaa
     5461 cggccgcctg gatccgaccg gccgaggtca ggggcgaatc gaggcgtccc tgcatgcgct
     5521 ttgccacatt ccactcagtc tggccgtgac ggatcagata gagccgcttc atgattcccg
     5581 ttggagatcc cggcaccgga gcccgtcagt cctcggcctt gccggacgcg gaggtccggt
     5641 aatctccgaa agcctcatcg cgtttcgaca aagcgtgggt cagccccttg ctcatctctt
     5701 tgaagtacgc acgcgtggtg tccgtgaaga aggcgagctg ctgcatctcc gtgccggcac
     5761 gaatgccggc ccggattccc atggcgtcca tctgccgatg cacggcgcgt ttgttgaatt
     5821 gctgcagatc cgtgggcgtg agggcgacgc gctcggctat cgccagaacc cgctcctcca
     5881 gctcctcggc gggaaaggca cggttggcaa agccgctttc tgccgcttcg acccccgaca
     5941 tgtgatcacc ggtgagcatg agctccatgg cccgcctgag gccgacgatc caggggtaga
     6001 actgattgtc cgggggcgac atggtgcgca cgaccggata gccgatcttg gcatcctccg
     6061 ccacgtaaac caggtcgcag gccgtggcaa gctcggtacc gccggccagg cagtatccgt
     6121 gcacctgggc aatgatcggc ttggccatgt cccacatgcg gaagaatcct tccaccacgt
     6181 gccgcggcca gtgaccaagg ccgccggccg tgtagaacgg cgggttctgg ctggtatcgc
     6241 tcttcagatc gtagcccgcc gagaagcacg gaccggcccc gcgtatgatg gtgacccgaa
     6301 ccccatcgtc ctgatcggcg ctttccagcg cgtggaacat ctccgagcgc agctcgttgg
     6361 agagcgggtt gcgcttctcg ggccggttga gcgttatgcg gcggaccatg ggccgcggct
     6421 cgtcgacgat gatcttctgg tattccatga tctcctcact gtcttcggat cgacggaccc
     6481 ggagggtgcc tatttcaaac tgaacccgtc tgccgtgaac gcggcgaacg ttcgctccac
     6541 gtcttcgggc gaggcggcca ggggagcgac gatgtgggtg t
//
LOCUS       DBNHQR010000627        16258 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_378411, whole genome shotgun sequence.
ACCESSION   DBNHQR010000627 DBNHQR010000000
VERSION     DBNHQR010000627.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 16258)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16258)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16258
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_378411"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="AC16VR_14305"
     CDS             complement(<1..261)
                     /locus_tag="AC16VR_14305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012380.1"
                     /translation="MPEVSPSSPEALLQQADLANGREAARLYLSATESFIQANRLDEA
                     ARSFQRIDQALLSGARTADYRYAAAEIALHAGEPDRAAALLGA"
     gene            356..1174
                     /gene="rsmI"
                     /locus_tag="AC16VR_14310"
     CDS             356..1174
                     /gene="rsmI"
                     /locus_tag="AC16VR_14310"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIR6012381.1"
                     /translation="MSGVLYVVATPIGNLDDITRRGADVLASVQIVAAEDTRRSQVLL
                     AAIGATQAELVSFHAHNEQNVARRLMAALQAGGDVALVSDAGTPLVSDPGFDLIRRCW
                     EEGVRVVPVPGASSVLAALSVCPLPAGRFFFEGFLPARAGQRRERLRHLAGRTEAVVF
                     FEAPHRVAACLADLEQLVPQRRLMVGREMTKRHESLHCGTPGELREALSSTLRGEFVF
                     VMEAAAGAGLDADAAEVMEILCAELAPAQAARLGSRLLKMDRSVLYRIALEQRS"
     gene            1215..1593
                     /gene="rnpB"
                     /locus_tag="AC16VR_14315"
     ncRNA           1215..1593
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="AC16VR_14315"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            1910..2368
                     /gene="mraZ"
                     /locus_tag="AC16VR_14320"
     CDS             1910..2368
                     /gene="mraZ"
                     /locus_tag="AC16VR_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HIR6012382.1"
                     /translation="MFRGINSATLDGKGRMALPTRHRESVVAVSEGRLVVTIDMREKC
                     LLMYPLPEWEVVQRKLESLSNIGEQARLLQRLLIGHATDLELDGQGRVLLPQMLRGYA
                     ALEKKLVLVGQGNKIEIWSDSLWQSRMNTWLSDGAGELLANGDEFTGLSV"
     gene            2365..3297
                     /gene="rsmH"
                     /locus_tag="AC16VR_14325"
     CDS             2365..3297
                     /gene="rsmH"
                     /locus_tag="AC16VR_14325"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HIR6012383.1"
                     /translation="MSNTHEPVLCQETVATLTGMGNSTVRQGVYVDATFGRGGHSRAL
                     LAVLGDESRVVALDRDPAAVAAGREMASSEPRLTICHARFSDLRRALASLDIESVQGV
                     LMDLGVSSPQLDDPQRGFSFLRSGPLDMRMDPGAGMSAAQWLNSASEQEIAGVLRSLG
                     EERYARRIAAAIAAARPISDTGELVALVRAAQPRATPGKHEATRVFQAVRMHVNDELG
                     ELESGLRQAFEMLSAGGRLAVIGFHSLEDRMVKRFFKSLTEPPPLPRRLPVRAAAQVV
                     PARSVAGPVRAGAAERARNPRSRSAVLRVVEKIS"
     gene            3294..3560
                     /gene="ftsL"
                     /locus_tag="AC16VR_14330"
     CDS             3294..3560
                     /gene="ftsL"
                     /locus_tag="AC16VR_14330"
                     /inference="COORDINATES: protein motif:HMM:NF016860.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HIR6012384.1"
                     /translation="MSRLYGWIVATLLVAVTVSGVQVAFSSQKVRQLHADLQAAQRVQ
                     DEELAEHSRLLLERSAQTSYVNVERVAEAELGMIFPDRVEPIEP"
     gene            3557..5209
                     /locus_tag="AC16VR_14335"
     CDS             3557..5209
                     /locus_tag="AC16VR_14335"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIR6012385.1"
                     /translation="MKGWRHYTLVGGFALGCVAVIVRLGYLNWTEREFLQEQGDARSV
                     RSEVLPAYRGVVYDRHGEPLAVSTPVFAVWLDPSRAGLTDESLGALAELLELDPARLS
                     ASVQSNGHREFLYLKRRVPWDRAQAVRELGISGVHLQSEYRRYYPASETTSHVVGTTG
                     IEDSGLEGIELSFDGRLRGEHGRKIVLKDQRGETIKDLDYVASPKFGDDLNLSLDLRL
                     QFLAYRELKSAVVGHRATSGSLVMLDARSGEILALVNQPSYNPNELGSVEVGNMRNRA
                     VTDVYEPGSTVKPFTVLAALESGRFAADTVIDTGPGYFRVGGKLVQDPVNRGAITVGE
                     ALQVSSQVGIAKIALDLEERAVYDVLSRAGVGEPVNTGLPGEAWASLDDAGLSSLVGR
                     ATLAYGYGLAISPLQLAQAYLTLATGGVRVPVSIVKQEVVRPGTRVFDADMIADVVGM
                     MEGVTDNDGTAPKARVVGYRVAGKTGTARKVGASGYDDERHVALFAGIAPVSDPRLVI
                     VVVVNEPKGDARGGGSVAAPVFGRVAARSMRLLGVRPDGESA"
     gene            5206..6654
                     /locus_tag="AC16VR_14340"
     CDS             5206..6654
                     /locus_tag="AC16VR_14340"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007162059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HIR6012386.1"
                     /translation="MSVSLRQLLEDPDAPEVPIRGLAVDSREVRPGDAFVAISGDRSD
                     GQDYVAEACRNGAVAVLSERPLAAGLELEGCPAVVVPDLKLRRGALAARVHQWPSRRL
                     RCIGVTGTNGKTSIAHFIADLAGRLGRTAGYMGTIGWGRPGALERTRLTTEDGVTVQR
                     RLGQLLDAGCDWVAMEVSSHALSQGRVAEVEFDVAVLSNLSRDHLDYHQTEAAYADAK
                     ASLFRWPSLDTAVVNLDDAFGRRVARSLGPGVALLGYGRQAELGWHDLVFTDQGVRGR
                     WRSPWGAADLALPLAGEFSVANMAAAIGALCCSGLAFDDVVEAAASVAQVPGRMEFLH
                     APGRPAVVVDYAHTPDALDKVLEALRRHTSGALVCVFGCGGDRDPGKRPLMAQAAERR
                     ADVIWMTSDNPRSEDPERIIDDMAAGLRGAACTHRCVDRREAIARAVENASAGDLVVV
                     AGKGHEDYQEVAGRRLSYSDRVEVANALGVNV"
     gene            6654..7736
                     /gene="mraY"
                     /locus_tag="AC16VR_14345"
     CDS             6654..7736
                     /gene="mraY"
                     /locus_tag="AC16VR_14345"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008045924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIR6012387.1"
                     /translation="MLLWLTEYLSGYVSGFGVFHYLTFRTMVSVMTALAMSLLIGPYV
                     IGKLSLYQIGQTVRDDGPESHFSKAGTPTMGGALILIVILVTTLLWGDLGNKYVWVVL
                     LVTVAFGVVGWIDDYLKLSQRSSRGLIARWKYFWQSLVGLGAAAFLYYTAEAPQETEL
                     IVPFFKEVAIPLGVGYLAVAYFVIVGTSNAVNLTDGLDGLAILPTVMVGAALGLIAYL
                     VGHTEFSSYLQIPYIADAGELAVFCGALIGAGLGFLWFNTYPAQVFMGDVGALALGAA
                     LGVVAVIVRHEIVLFIMGGLFVLETVSVILQVASYRMTGRRIFRMAPIHHHFELKGWP
                     EPRVIVRFWIVTLVLVLIGLSTLKLR"
     gene            7756..9063
                     /gene="murD"
                     /locus_tag="AC16VR_14350"
     CDS             7756..9063
                     /gene="murD"
                     /locus_tag="AC16VR_14350"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIR6012388.1"
                     /translation="MKRTAIIGLGVTGLSCLEYLHGEDELVVLDTRAEPPHAGVVPDY
                     PDTEFHFGLAGFDFAGVDRVIVSPGVELDSCLVRAARAAGAELASDIDLFCEAAQAPI
                     TAITGTNGKSTVTALMGHLLRATGGNPGVGGNLGEPALSLLDEACDSYVLELSSFQLE
                     RLRPWRFSAATILNVTEDHLDRHRSLAEYAASKQRVYRDCGRAVANRADPLTFPQHDV
                     DELVTFGGDAPGEGDWGVVDGVLARGERAVLPVEALPLSGRHNELNVLAACALAWHPR
                     ADLQSLAGAVSSFRGLPHRCQLVAEAGGVRYVNDSKATNVGATVAALQGLGESSGGSL
                     ILIAGGDGKGADFTALREPVSRYVKTAVLLGVDADAMARALDGAVPLVRVDTLEAAVV
                     AARNAAGPGDLVLLSPACASLDMFENFADRGERFAAAVMEFAA"
     gene            9060..10145
                     /gene="ftsW"
                     /locus_tag="AC16VR_14355"
     CDS             9060..10145
                     /gene="ftsW"
                     /locus_tag="AC16VR_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007162055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HIR6012389.1"
                     /translation="MTARLDMTLLLSWLLILVLGTVMVASAAVAISESLVWKHGIFLL
                     VSLTAFGVVLAVPLAVWQRCHHLAWGLAVILCILVLVPGIGVEVNGARRWIGLGAFTL
                     QASEFAKLLVLVYVAGYLARFDEAVRESSIALLRPLMMVAIVALLLLMEPDFGSVVVL
                     GAAVCGMLFVAGTRLRHFLLLVVVAALLLAALALLQPYRMQRLVSFTDPWSFAFGSGY
                     QLTQALIAFGRGEVTGLGLGEGIQKLFYLPEAHNDFIFAVIAEELGLLGTVAVVVLFG
                     TLIARVLLIAGRAVAEERYFAGYLTYGVGLLLGVQCLVNMGVNTGMLPTKGLTLPFVS
                     FGGNSLLVCSTMLGLAFRAQMEAKGRG"
     gene            10165..11553
                     /gene="murC"
                     /locus_tag="AC16VR_14360"
     CDS             10165..11553
                     /gene="murC"
                     /locus_tag="AC16VR_14360"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIR6012390.1"
                     /translation="MRRVRRIHFVGIGGAGMCGIAEVLLNQGYQVSGSDLRASETTAR
                     LERLGATVHVGHAAGHVEGKDVVVVSSAVAGDNPELRAAHARRIPVVARAEMLGELMR
                     YRHGIAVAGTHGKTTTTSLITAIFQTAGLDPTFVIGGLLNSTGTNAQLGAGRYLIAEA
                     DESDASFLHLKPMQAVITNIDRDHMGTYHNDFAELRGAFVRFVHRLPFYGSVVLCTDD
                     ANVRDVLPELARPMLTYGFAEDGDYRARDLDVNGASWRFVAERPDGHGDLEISLAVPG
                     EHNVLNALAAAAVATDEGIPDEAIVAGLSQFRGVGRRFEVFAQTPVGCARVTMVDDYG
                     HHPSEVAAVIQTARRVWPDRRLVMVYQPHRFTRTRDLYDDFVRVLSEVDALVLLEVYA
                     AGEEPIPGADGKALCQGLRQRGLVNPTYAADPEEALGLLPDIVRDGDVVLVQGAGSVN
                     QISLSLKEVTDA"
     gene            11546..12250
                     /locus_tag="AC16VR_14365"
     CDS             11546..12250
                     /locus_tag="AC16VR_14365"
                     /inference="COORDINATES: protein motif:HMM:NF015739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIR6012391.1"
                     /translation="MRRVSAVLLIVLAGAVVAALVWQMDRPIENVRVIGELTPGEQQQ
                     IEAAVLTGLDAGLLSVDLAEVRDRLRSLSWTRRVTVRRVWPPGLDIAVDKDVPVAVWG
                     DGAHLTSNGRVIAIPDAPAALPVFDCALASPRSALEVYLLLQESLPGAGRRISALRQT
                     SLGEWEVELRGGLRVALGDRDIAQRIARFGIVYERALKDRIETVDYVDARYTGGLAVG
                     WKDPLLAYEEPTANGI"
     gene            12240..13469
                     /gene="ftsA"
                     /locus_tag="AC16VR_14370"
     CDS             12240..13469
                     /gene="ftsA"
                     /locus_tag="AC16VR_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HIR6012392.1"
                     /translation="MASDSELTRIVGLDIGTSKVAAIVAELNLEGELEIIGLGTHPSR
                     GLKKGVVVNIESTVQSIQRAVEEAELMAGCHIENVYAGIAGSHIRSINSHGIVAVRDR
                     EVYPQDVDRVIDAAQAMAIPADQKILHILPQEYVIDNQEGVKEPLGMSGVRLEAKVHL
                     VTCAVNAAQNIEKCIVRCGLTVNDIILEQLASSYSVLTDDERDLGVCLVDIGGGTTDI
                     AVFTGGAIRHTAVIPIAGDQVTNDIAMALRTPTQNAEEIKIRYACALTQLAGPDETIK
                     VPGVGDKPAKELSRQALAEVVEPRYDELFTLIQAELRRSGFEDLIAAGIVLTGGASKM
                     EGVVELAEEIFHMPVSLGAPRDVAGLKDIVRNPVYATGVGLLQYGREREREMPGRGRL
                     RGKGAFGRMKKWLSDNF"
     gene            13483..14631
                     /gene="ftsZ"
                     /locus_tag="AC16VR_14375"
     CDS             13483..14631
                     /gene="ftsZ"
                     /locus_tag="AC16VR_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIR6012393.1"
                     /translation="MFELVDSAPQSAVIKVVGVGGGGGNAVQHMLSRQVEGVDFIAAN
                     TDAQALKHLDAKTILQIGSNITKGLGAGANPDIGRQAAIEDRDRLGEVLVGADMVFIT
                     AGMGGGTGTGAAPVVAEVARELNILTVAVVTRPFKFEKRSDVADRGIAELRETVDSLI
                     TIPNEKLLAVLGERTTMLDAFGAANDVLLGAVQGIADLIIRPGIINVDFADVRTVMSE
                     MGMAMMGTGRGTGDNRARDAAEAAIRSPLLEDVDLHGARGILVNVSAGPDLTIGEYSD
                     VGDIINEFASEQATVVVGTVIDQDLGDELKVTVVATGLGEPARPNVVIDNTVIEEVIQ
                     GQIDYRDLDQPTYKRHNQALHKDNLAEDLDTQDLDYLDIPAFLRRQAD"
     gene            14834..15748
                     /gene="lpxC"
                     /locus_tag="AC16VR_14380"
     CDS             14834..15748
                     /gene="lpxC"
                     /locus_tag="AC16VR_14380"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103117 - UDP-3-O-acyl-N-acetylglucosamine
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HIR6012394.1"
                     /translation="MALNQKTLKNSIRATGVGLHTGDKVFLSLRPAPVDTGIVFVRTD
                     LDGEPRLPARVENVGDTTMATSLTNGNVSVSTVEHLMSAFAGLGIDNAFVDVSAPELP
                     IMDGSAAPFVFLIQSAGVEEQTAPRRFVRIKKAVAFRQGDVVAELKPFDGFRVSYTLV
                     YDHPVFRKHERSATVDLSNTAYVKEVSRARTFGFLADYEQLRSMNLARGGSLDNAVVV
                     DDYRILNGEGLRQADEFVKHKILDAVGDLYLLGCGLIGEFCGYKSGHGSNNALLRRLL
                     ADPEAFEIVTFANEADAPTGFAAPVVAN"
     gene            complement(15849..>16258)
                     /locus_tag="AC16VR_14385"
     CDS             complement(15849..>16258)
                     /locus_tag="AC16VR_14385"
                     /inference="COORDINATES: protein motif:HMM:NF017103.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HIR6012395.1"
                     /translation="PDRNPKRWRNEPTRAKIHSPRAADTVVHDRDPAMANLKVDLLLS
                     ESQRRFTPLQRLLRQSANQESWTAGLLALLPASLQNDCRVSDIRNGSIIVVCRNAATA
                     TRLRFLAPELLSKLHDLADFRVAQRIEVRVSEF"
BASE COUNT         2621 a         5046 c         5641 g         2950 t
ORIGIN      
        1 ggcgccaagc agcgcggccg cccgatccgg ctcgccggcg tgcagcgcga tctcggcggc
       61 ggcatatcga tagtcggccg tccgggcgcc ggagagcaac gcctggtcga tcctctggaa
      121 agatcgggca gcctcgtcga gccggttggc ctgaatgaag ctctcggtgg ccgacaggta
      181 cagccgggct gcttcccgcc cgttggccag atccgcctgt tgcaggagag cttcgggaga
      241 tgagggggag acttcgggca cgttcgcttc gattgtctcg agcggcgtgc tttgacaggc
      301 ggacactgcg agaatagcga acgcccaagc ccaggccctt atccggtttg ctgccatgtc
      361 tggtgttctc tacgtcgttg ccacgccaat tggaaacctc gacgacatca ctcgtcgagg
      421 cgccgatgtc ctggcgtcgg tgcagatagt cgccgccgag gataccagac gctctcaggt
      481 gctgttagca gcaatcggcg caacccaggc cgagctggtc tcgttccacg cgcacaacga
      541 gcagaacgtg gcgcggcgtc tcatggccgc gctgcaggcg ggtggcgatg tggcactggt
      601 ctccgacgcc ggcacgcccc tggtatccga cccgggcttc gatctcatcc ggcgctgctg
      661 ggaggaaggc gtccgggtgg ttcccgtccc cggcgcgagc tcggtcctgg ctgctctgtc
      721 cgtctgtcct cttccggccg gccgcttttt cttcgagggc tttctgccgg caagggcggg
      781 gcagcgccgc gagcggctgc ggcatcttgc cggacgcacg gaagccgtcg tcttcttcga
      841 ggcgccccac cgggtcgccg cctgccttgc ggatctggag cagctggtcc cgcagcggcg
      901 actcatggtc gggcgggaaa tgaccaaacg tcacgagagt ctgcactgcg gcaccccggg
      961 agagctgcgc gaggcgctgt cgagcaccct tcggggggag ttcgtcttcg tgatggaggc
     1021 cgccgcggga gctggcctcg acgcggacgc cgccgaggtc atggagatcc tttgtgccga
     1081 gctggctccg gctcaagccg cacggctcgg ctcgcgcctg ctcaagatgg accgctccgt
     1141 gctctaccgg atcgccctgg agcagcgctc ctgagggcct ggcaatccgc cggtccgggg
     1201 attagtcttg ccctcgagtc ggccagacag tcgctggcag catcagctgc gagaggaaag
     1261 tccgggctcc acaggacgag gtgccaggta acgcctgggg ggcgcgagcc cacggaaagt
     1321 gcagcagaaa gcaaaccgcc taaggcgcgg cgctccggcg ctgcgaacgg taagggtgaa
     1381 agggtgcggt aagagcgcac cgcgcgcgtg gtaacactgc gcggctaggt aaaccccgcc
     1441 tggagcaagg ccaaatagga accccatggt gtggtccgca ccgggttcgg gtaggtcgca
     1501 cgaggcgttc ggtgacgaac gccctagatg aatgactgtc ggctgcgttc tcggacgcgg
     1561 caacagaacc cggcttatcg gccgactcgt ttcttttcgc acctaaaacg ggatttttaa
     1621 tgccgattgg gtctagaagt taaggtgaat tctagatacc tgcttctacc tgaggttaac
     1681 taagaagata aagctgcccg ctgccttctg acagcctctc tgcgccgaaa aaatccataa
     1741 atttccgtag gttatcgacg ttttcctcgc ttgacaggca agcctgtggg tcgatatcgt
     1801 ggctggtggt gtgccaattt gggatttagt gggagattgt gggagattcc gtctgaatcg
     1861 gtcgtgacct ggttcatttt gaccgattcg cgcggccccc agggcagtca tgtttcgagg
     1921 catcaacagc gccactctgg atggcaaggg gcgcatggct ctgccgaccc gtcatcgcga
     1981 gtcggtggtc gccgtgagcg aggggcgcct ggtcgtgacc atcgacatgc gggaaaagtg
     2041 cctgctgatg tacccgcttc cggaatggga ggtggtgcag cgcaagctcg aaagcctttc
     2101 gaacatcggt gagcaggccc ggttgctgca gcggctgctg atcggccatg ccacggacct
     2161 cgagctggac ggccagggtc gggtgctgct gccgcagatg ctcagaggct atgcggcatt
     2221 ggaaaagaag ctggtgctgg tcggtcaggg caacaagata gaaatttgga gcgattcgct
     2281 gtggcagagc cggatgaaca cctggctctc ggatggggct ggggaactgc tggccaacgg
     2341 cgacgagttc accggattgt cggtttgagc aatacgcacg agccggtgct ttgccaggag
     2401 acggtggcga cattgacggg gatggggaac tcgacagtca ggcagggcgt gtacgtcgac
     2461 gccacgttcg ggcgcggtgg ccattcgcgc gcgctcctgg cggttcttgg tgacgagagt
     2521 cgggtggttg ccctggatcg ggacccggcg gccgtcgcgg cgggaaggga gatggccagc
     2581 tccgagcccc gtttgaccat ttgtcacgcg cgcttttctg atctgcgccg ggcgcttgcc
     2641 tcgctcgaca tcgagtcggt gcagggcgtg ctcatggatc tcggcgtttc ctctccccag
     2701 ctggatgatc ctcagcgagg tttcagcttt ctgcggtccg ggcccctgga catgcgcatg
     2761 gacccgggcg cgggaatgtc tgccgcgcag tggctcaatt cggcttcgga gcaggagatc
     2821 gccggcgtgc tgcgatcgct cggagaggag cgctatgcca ggcgcatcgc cgccgccatc
     2881 gcggcggccc ggccaatctc cgataccggt gagctggtgg cgctggtgcg cgcggcgcag
     2941 ccgcgagcca cacccggcaa gcatgaggcc acccgcgtgt tccaggcggt gcggatgcac
     3001 gtcaacgatg agcttggcga gctcgagagc gggttgcgac aggcattcga gatgctatct
     3061 gccggggggc gcctggcggt gatcggcttt cactctctgg aagaccggat ggtcaagcgc
     3121 ttcttcaaga gcttaacgga gcccccgccg ttgccgcgcc ggttaccggt aagggcggcg
     3181 gctcaggtcg tcccggcgcg ctcggtggca gggcccgtcc gcgctggcgc tgccgaacgg
     3241 gcgcgcaacc cgcgctcgcg cagcgccgtc cttcgggtgg tggagaaaat ctcatgagtc
     3301 ggctttacgg ctggatcgtg gcaaccctgc tggtcgcggt cacggtgtcc ggcgtgcagg
     3361 ttgcgttcag cagccagaaa gtgcggcagc ttcacgccga tcttcaggcc gcgcagcgtg
     3421 ttcaggacga ggaactcgcc gagcacagcc ggctgctcct ggagcgcagc gcgcagacct
     3481 catacgtgaa cgtcgaacgg gtggcggaag ccgagcttgg catgatcttc cccgaccggg
     3541 tggagcccat cgagccatga agggctggag gcactacacg ctggtgggag gcttcgctct
     3601 gggctgcgtg gccgtgatcg ttcggctcgg ttacctcaac tggaccgaac gggagttcct
     3661 gcaggagcag ggcgatgcca gatccgttcg cagcgaggtg ctgccggcct atcgcggtgt
     3721 cgtctacgac cgccatggcg agccgctggc agtcagcacc cccgttttcg cggtctggtt
     3781 ggatccaagc cgtgctggcc tgacggacga gtcgctgggc gcgcttgccg agttgctcga
     3841 actggacccc gctcgcctgt ccgcgtccgt gcagagcaac ggccatcggg agtttctcta
     3901 cctcaagcgc cgggtcccct gggaccgggc gcaggccgtc agggagctgg gcatctcggg
     3961 cgttcacctg cagtccgagt atcgccgcta ctacccggcc agcgagacga cgtctcacgt
     4021 ggtcggcacc accggcatcg aggattccgg gctggagggg atcgagctct ccttcgacgg
     4081 ccggcttcgc ggcgagcacg gacggaagat cgtcctgaag gatcagcgcg gcgagaccat
     4141 caaggatctc gattacgtcg cctcgccgaa attcggcgac gacctgaacc tgagcctcga
     4201 tctgaggctg cagtttctcg cctatcggga gctcaagtcc gcggtggtgg gtcatcgtgc
     4261 cacgtcgggc tccctcgtca tgctggatgc gcgcagcggc gagattctcg ccctggtcaa
     4321 tcagccgtcc tacaacccca acgagctcgg ctcggtggaa gtggggaaca tgcgcaaccg
     4381 cgcggtcacg gacgtgtacg agcccgggtc caccgtaaag ccgttcacgg tgctcgccgc
     4441 cctcgagtcc ggtcgcttcg ccgccgatac ggtcatcgat accgggccgg gttacttccg
     4501 ggttggcggc aagctggttc aggacccggt caaccggggt gccatcaccg tcggcgaagc
     4561 gctgcaggta tccagccagg tgggtatcgc gaagatcgcg ctggatctgg aggagcgggc
     4621 cgtgtacgac gttctcagtc gtgccggtgt gggtgagccc gtcaataccg gcctgccggg
     4681 cgaggcctgg gcgtcactcg acgacgcggg cctctccagc ctggtgggcc gtgccacgct
     4741 ggcctacggt tacggtctcg ccatctcgcc cctgcagctc gcacaagcct acctcacgct
     4801 tgcaaccggt ggcgtccggg tgccggtgtc catcgtgaag caggaagtcg tgcgtccggg
     4861 cacgcgcgtg ttcgatgcgg acatgattgc ggacgtcgtc gggatgatgg agggcgtcac
     4921 cgacaacgac ggcaccgcgc ccaaggcccg cgtggtgggc taccgggtgg caggcaagac
     4981 gggcactgcc cgcaaggtgg gcgcctcggg ctacgacgac gagcgccacg tcgcgctgtt
     5041 tgccggaata gcgccggtat cggatccgcg actggtcatc gtcgtggtgg tcaacgaacc
     5101 caagggcgat gcccgcggcg gcggcagcgt tgccgcgccg gtgtttggtc gggtggccgc
     5161 gcgctccatg cgtcttctgg gcgtacgccc ggacggggag tcagcgtgag cgtatccctg
     5221 cggcagctgc tcgaagaccc cgacgccccc gaggtgccga tcagggggct ggcggtggac
     5281 tcccgggagg ttcgtccggg ggatgcgttc gtggcgatct ccggggatcg ttcggacggg
     5341 caggattacg tcgccgaagc ctgccgtaac ggcgcggtgg ccgtgctgtc ggagcgaccg
     5401 ctggcggcgg ggctggagct ggaaggctgc ccggccgtgg tcgtgcccga tctcaagctg
     5461 cgccggggcg ccctggcagc tcgcgtgcac cagtggccga gccgccggct gaggtgtatc
     5521 ggggtgacgg ggacaaacgg caagacgtcc attgcccact tcatcgccga tctggccgga
     5581 cgcctcggcc ggaccgccgg ctacatgggc accatcggct ggggcaggcc cggggcgttg
     5641 gaacgcacga ggctcaccac cgaggatggg gtgaccgttc agcgccgtct gggccagctt
     5701 ctggatgcgg gctgcgattg ggttgccatg gaagtcagct cccacgccct cagtcagggg
     5761 cgggttgccg aggtggagtt cgacgtcgcc gtgctgtcca atctgtcccg cgaccacctc
     5821 gactaccacc agaccgaggc ggcctacgcg gatgccaagg catccctgtt ccggtggccg
     5881 tctctcgaca cggcggtggt caatctcgac gacgcgttcg gccgtcgcgt ggcgcgatct
     5941 cttgggcccg gcgtcgccct gctcggctac ggccgccagg cggagctcgg ctggcacgat
     6001 ctcgtcttca ccgaccaggg cgttcgcggc cgctggcggt cgccctgggg cgccgccgat
     6061 ctggcgttgc ccctggccgg cgagttctcg gtggccaaca tggcggccgc cataggagcg
     6121 ctgtgctgct ccgggctcgc cttcgacgac gtggtggaag cggcggcctc ggttgcccag
     6181 gttcccggtc ggatggagtt tcttcacgcg ccggggcgcc ccgcggtggt ggtggactac
     6241 gcccacacgc cggacgctct ggacaaggtg ctcgaggcgc tcaggcgcca cacgtccggc
     6301 gcgctggtgt gcgtgtttgg ttgcggcggc gaccgtgatc cgggcaagcg gccgctcatg
     6361 gcccaggccg ccgagcgtcg cgcggacgtg atctggatga cgagcgacaa cccccggtcg
     6421 gaagatccgg agcggatcat cgacgacatg gcggcggggt tgcggggcgc ggcgtgcacg
     6481 caccgctgcg tcgatcgccg cgaggcgatc gcccgggcgg tggagaacgc ctcggcgggc
     6541 gacctggtgg tggtggcggg caaaggccac gaggactacc aggaagtggc cgggcgccgc
     6601 ctgagctaca gcgaccgggt cgaggtcgcc aacgcgctgg gagtgaacgt ctgatgctgt
     6661 tgtggctgac cgaatacctg tccggatatg tgagcggctt cggcgtgttc cactacctga
     6721 cattccgaac catggtgagc gtgatgacgg cgctggcgat gtcactgctg atcggtccct
     6781 acgtcatcgg caagctctcg ctttatcaga tcgggcagac cgtgcgcgac gacggtcctg
     6841 aaagccactt cagcaaggcg ggcaccccga ccatgggcgg ggcgttgatc ctgatcgtca
     6901 ttctggtcac cacgctgctg tggggagacc ttggtaacaa gtacgtgtgg gtcgtcctgc
     6961 tggtcacggt ggcattcggg gtcgtcggct ggatcgacga ctacctgaaa ctctcccagc
     7021 gcagcagccg cgggctcatc gcccggtgga agtatttctg gcagtccttg gtggggctgg
     7081 gggccgcggc ctttctctac tacaccgcgg aggctcctca ggagaccgag ctgatcgtcc
     7141 cgtttttcaa ggaggtcgcc atcccattgg gtgtgggcta cctggcggtc gcctacttcg
     7201 tcatcgtggg aaccagcaac gcggtcaacc tcaccgacgg gctcgacggc cttgccattc
     7261 tgcccacggt catggtgggc gctgccctgg gcctgattgc ctacctggtc ggtcacacgg
     7321 agttttccag ctatctgcag ataccctaca ttgccgacgc cggggagctg gccgtgttct
     7381 gcggcgcgct gatcggcgcc gggctcggct ttctctggtt caacacgtat ccggcccagg
     7441 tgttcatggg cgacgtgggt gcgctggcgc tgggtgcggc gctcggtgtg gtcgccgtca
     7501 tcgtgcgtca cgagatcgtg ctgttcatca tgggcgggtt gttcgtgctg gagacggtct
     7561 ccgtcattct tcaggtggcc tcgtaccgca tgaccggccg tcggatattc cggatggcgc
     7621 caattcatca tcatttcgag ctcaagggat ggcccgagcc gcgcgtgatc gttcggttct
     7681 ggatcgtcac cctggtcctc gttctgatcg gactttccac gctcaagctc aggtagcgga
     7741 cacggatcag ccagattgaa aaggacagcg atcatcggct taggcgtaac cggcctctcc
     7801 tgcctggagt acctccacgg cgaggacgag ctggtggtgc tcgatacccg tgccgaaccg
     7861 ccccatgccg gcgtcgtgcc cgactatccg gacacggagt ttcacttcgg cctggcgggc
     7921 tttgacttcg cgggcgtcga ccgcgtgatc gtcagccctg gggtggagct ggacagctgc
     7981 ctggtcagag cggcgcgtgc ggccggcgcg gaactcgcca gcgacatcga cctcttctgc
     8041 gaagcggcgc aagcgcccat caccgcgatc acgggaacca acggcaagag cacggtcaca
     8101 gcgctgatgg gccacctcct gcgtgccacg ggcggcaatc cgggcgtcgg cggcaatctc
     8161 ggtgagccgg cgctttccct tctcgacgag gcctgcgaca gctacgtgct cgagctctcc
     8221 agcttccagc tggagcgtct tcgcccctgg cggttcagcg cagcgaccat cctcaacgtc
     8281 accgaagacc atctggaccg acaccgcagc cttgccgagt acgcggccag caagcagcgc
     8341 gtctatcggg attgcgggcg ggccgtagcc aaccgggcgg accccctcac ctttcctcag
     8401 cacgacgtgg atgagctggt caccttcggc ggcgatgcgc cgggcgaggg tgattggggc
     8461 gtcgtcgacg gagtgctcgc ccgtggcgag cgcgctgtgc tgccggtgga agcgcttccg
     8521 ctgtccggcc ggcacaacga gttgaacgtg ctcgctgcct gtgcgctcgc ctggcacccg
     8581 cgtgcagatc ttcaatcgct ggcgggagcg gtatcgagct ttcgcgggct gccgcaccgc
     8641 tgccagctgg tcgccgaggc cggtggcgtg cgctacgtga acgactccaa ggcgacgaac
     8701 gtcggcgcca ccgtcgcggc gctgcagggc ctgggagagt cctccggggg atcgctgata
     8761 ctgattgcgg gcggggacgg caagggtgcg gatttcaccg ctctgcggga gccggtctcg
     8821 cgctacgtga agacggccgt tctgctcggc gtggatgccg acgccatggc tcgggctctg
     8881 gacggcgccg ttccgctcgt cagagtggat accctggagg ccgcggtggt cgcggcccgc
     8941 aacgcggcgg gtcccggcga tctggtcctg ctttcccccg cctgcgccag cctggacatg
     9001 ttcgagaact tcgccgatcg cggtgagcgc ttcgcggcgg cggtcatgga gttcgcggca
     9061 tgacggcgag gctcgacatg acgctcctgc tgtcctggct gctcatccta gtgctgggca
     9121 cggtcatggt ggcctccgcc gccgtcgcca tcagtgaaag cctggtctgg aagcacggca
     9181 tcttcctgct cgtcagcctg accgcgttcg gcgtcgtgct cgcggtgccg ctcgccgttt
     9241 ggcagcgttg ccaccacctc gcctggggcc tcgcggtgat tctctgcatc ctggtgctgg
     9301 ttcccggcat cggcgtcgag gtgaacggcg cgcggcgctg gatagggctt ggcgccttca
     9361 cgctgcaggc gtcagagttc gccaagctcc tggtgctggt ctacgtggcc ggctatctgg
     9421 cccgctttga cgaagcggtg cgggagagct ccatcgcgct gctgcggccg ctcatgatgg
     9481 tggccatcgt cgcgctgctg ctgctcatgg agcccgactt cggctccgtc gtggttctcg
     9541 gcgccgccgt ctgcgggatg ctcttcgtcg cgggcacccg gcttcgtcac tttctcctgc
     9601 tcgtcgtggt cgcggctctg ctgctggccg cccttgcgct gctgcagccc taccgcatgc
     9661 agaggctggt gagcttcacg gacccgtggt cgttcgcctt cggcagcggc taccagctca
     9721 cccaggcgct gatcgccttc gggcgtggag aggtcaccgg gctcggtctt ggcgagggta
     9781 tccagaaact cttctacctg ccggaagcgc acaacgactt catattcgcc gtgattgccg
     9841 aggagctcgg cctgctgggc accgtcgcgg tggtcgttct gttcggcacg ctgatcgcca
     9901 gggtgctgct catagccggt cgggcggtgg cggaagaaag atacttcgcc ggctacctca
     9961 cctacggcgt cggcctgctg ttgggcgtgc agtgccttgt caatatgggc gtcaacaccg
    10021 ggatgttgcc gaccaaaggc ctcacgctgc cattcgtgag cttcggtggt aacagcctgc
    10081 tggtgtgcag caccatgctg gggctcgcgt tccgggccca gatggaggcg aaaggccgtg
    10141 gctgagtcgt actacgtgcc ggagatgcgc cgggtgcggc gcattcactt cgtgggcatt
    10201 ggcggtgccg gcatgtgcgg cattgccgag gtgcttctca accagggata ccaggtgtcg
    10261 ggatcagacc ttcgcgcgtc ggagaccacg gcgcgactgg aacggctcgg cgcaaccgtc
    10321 catgtgggcc atgctgcggg gcacgtcgag ggcaaggacg tggtcgtggt atccagcgcg
    10381 gtcgcgggag acaaccctga gcttcgcgcc gctcacgcgc ggcggatacc ggtcgtggcg
    10441 cgggcggaga tgctcgggga gctcatgcgc tatcgtcacg gcattgccgt cgccggaacc
    10501 cacggcaaga cgaccactac cagcctgatc acggccatat tccagaccgc gggcctggac
    10561 cccaccttcg tcatcggcgg tttgctcaac agcacgggaa ccaatgcgca gctgggcgcc
    10621 ggccggtacc tgattgcgga agccgacgaa agcgacgcgt cgtttcttca cctcaaaccc
    10681 atgcaggccg tgatcaccaa catcgaccgg gatcacatgg gcacctacca caacgacttt
    10741 gccgagctgc gcggcgcgtt cgtgcggttc gtgcaccgtc tgcccttcta cggaagcgtc
    10801 gtgctgtgca cggacgatgc gaacgtgcgc gacgttctgc ccgagcttgc ccggcccatg
    10861 ctgacttacg gcttcgcgga ggacggcgac taccgcgccc gggatctcga cgtgaacggc
    10921 gcatcgtggc gtttcgtcgc cgagcgtccc gacggtcacg gtgacctgga gatctccctg
    10981 gccgttcccg gcgagcacaa cgtgctcaac gcgctcgcgg cggcagcggt cgccaccgac
    11041 gaaggcatac cggacgaggc catcgtcgcc ggcctgtcgc agttccgcgg cgtgggccgg
    11101 cggttcgagg ttttcgcgca gacgccggtg ggctgcgccc gcgtcacgat ggtcgacgac
    11161 tacggccacc atccttccga agtggccgca gtgattcaga ccgcgcgccg cgtctggccc
    11221 gatcggcggc tcgtcatggt ctatcagccg caccggttca cccgtaccag ggatctttac
    11281 gacgatttcg tgagagttct ctccgaggtg gacgccctcg tgctgctgga ggtttacgcg
    11341 gcgggcgagg aacccatacc tggcgcggac ggcaaggcgt tgtgccaggg cctgcggcag
    11401 cgcgggctcg tcaaccccac ctacgccgcc gaccccgagg aagccctggg gctcctgccc
    11461 gacatcgtcc gggatggcga cgtggtgctc gttcaggggg ccggcagcgt caaccagatc
    11521 tcgctgagcc tcaaggaggt gacggatgcg tagggtgtcc gccgtgctcc tgattgtgct
    11581 ggccggggcc gtggtggctg cgctggtgtg gcaaatggat cggcccatcg agaacgtgcg
    11641 cgtcatcggc gagctcacac ctggggagca gcagcagatc gaggcggcgg tgctcaccgg
    11701 cctcgatgcg ggcctgctga gcgtggatct cgccgaggtg cgtgaccggc tgcggagcct
    11761 ttcctggacg cgtcgcgtga cggtccgccg cgtatggcct ccggggctcg acattgccgt
    11821 cgacaaggat gttcctgtcg cggtctgggg cgacggtgct cacctcacca gcaacggccg
    11881 ggtgatcgcg atcccggacg cgcccgccgc gctgcccgtc ttcgactgcg cgctggccag
    11941 cccgcgcagc gcgctggaag tctatctgct gctgcaggag tccctgccgg gcgcgggaag
    12001 acgcatctcg gcgctcaggc agacgtccct cggcgagtgg gaagtcgagc ttcgcggcgg
    12061 tcttcgggtt gcgctgggcg accgggatat cgcccagcga atcgcccgat tcggcatcgt
    12121 ctacgaacgg gcgttgaagg accgcatcga gaccgtcgac tacgtcgatg cgcggtatac
    12181 cggcggtctc gcggttggtt ggaaagatcc gctccttgca tacgaggaac ccacagcaaa
    12241 tggcatctga cagtgagctg acccgaatcg tcggtctcga catcggcacg agcaaggtgg
    12301 ccgccatcgt ggccgagctc aacctcgaag gggagctcga gatcatcggg ctcggaacgc
    12361 acccatcacg gggattgaag aaaggcgtgg tggtgaacat cgaatccacc gtgcagtcca
    12421 tccagcgcgc cgtggaggag gccgagctca tggccggctg ccacatcgag aacgtctacg
    12481 cgggcatcgc cggcagtcac atacgaagca tcaactccca cgggatcgtc gccgtgcggg
    12541 accgcgaggt ctatccccag gacgtggacc gggtgatcga tgcggcccag gccatggcca
    12601 tcccggcgga ccagaagatt ctgcacatcc ttccccagga atacgtcatc gacaaccagg
    12661 agggggtgaa agagcccctc gggatgtcgg gggtgcggct cgaagccaaa gtgcatctgg
    12721 tcacctgcgc cgtgaacgcc gcgcagaaca tcgagaagtg catcgtgcgg tgtggcctga
    12781 ccgtcaacga catcattctc gagcagcttg cctcgagcta ctccgtgctt acggacgacg
    12841 agcgcgacct gggcgtctgc ctggtggaca taggcggcgg caccacggac atcgcggtgt
    12901 tcacgggcgg cgccattcgt cacacggcgg tgatccccat cgccggtgac caagtcacca
    12961 acgacattgc catggcgctg cgaacgccca cgcagaacgc agaggagatc aagatcagat
    13021 atgcctgcgc gctgacgcag ctcgccggtc ccgatgaaac catcaaggtt cccggggtcg
    13081 gcgacaagcc agccaaagag ctgtcgcgcc aggcgcttgc ggaggtggtg gagccccgct
    13141 acgacgagct gttcacgctg atacaggcgg agctgcggcg tagcgggttc gaagacctca
    13201 tcgccgcggg catcgtgctc accgggggag cgtcgaagat ggagggggtt gtcgagcttg
    13261 cggaagagat cttccacatg ccggtcagcc tgggcgcgcc acgtgacgtg gcgggtctga
    13321 aggacatcgt gagaaatccc gtatacgcga ccggcgtcgg cctgttgcag tacggcaggg
    13381 agagggagcg ggagatgccc ggtcgcggaa ggcttcgggg caagggtgct ttcggccgaa
    13441 tgaaaaagtg gttgagcgac aatttctagt taggagatca ggatgtttga actggtagac
    13501 agtgccccgc agagcgcggt gatcaaggtc gtcggagtgg gaggcggcgg tggcaacgcc
    13561 gtccagcaca tgttgagcag gcaggtagag ggggtggatt tcatcgccgc caataccgat
    13621 gcccaggctc tgaagcacct cgacgcgaag acaattctgc agatcgggag caatatcacc
    13681 aaaggcctcg gcgccggcgc gaatcccgac atcgggcggc aggccgccat cgaggatcgg
    13741 gatcgtctgg gtgaggtgct ggtcggggcg gacatggtgt tcatcaccgc cgggatggga
    13801 ggcggcacgg gaaccggagc tgcgcccgtg gtcgcggaag tggcccgtga gctgaacatc
    13861 ctgaccgtcg cggtggtcac ccggcctttc aagttcgaaa agcgctcgga cgtcgccgat
    13921 cgcgggatcg cggagctgcg tgagaccgtc gattccctga tcaccatacc caacgaaaag
    13981 ctccttgccg tgctcgggga gcgaaccacc atgctggatg cgttcggtgc ggccaacgac
    14041 gtgctgctcg gggccgtgca ggggattgcc gacctcatca tccggccggg aatcatcaac
    14101 gtggattttg ccgacgtgcg gacggtcatg tcggagatgg gcatggccat gatgggcacg
    14161 ggccggggaa cgggagacaa tcgcgcgcgc gacgcggcgg aagcggcaat ccgctcgcct
    14221 ctgctcgagg acgtggatct gcacggcgcg agaggcattc tggtcaacgt ctctgccggg
    14281 ccggatctca ccatcggcga gtactccgac gtgggcgaca tcatcaacga gtttgcctcc
    14341 gagcaggcca ccgtcgtggt ggggacggtc attgatcagg atctgggcga cgagctgaag
    14401 gtcaccgtgg tggccacggg gctcggcgag ccggcaagac ccaatgtggt catcgacaac
    14461 acggtgatcg aggaggtcat ccaggggcag atcgactacc gggatctgga ccagccgact
    14521 tacaagcgtc acaaccaggc tctgcataag gacaaccttg cagaggatct ggacactcag
    14581 gatctggact atctggacat tccggctttc ctgcgtcgcc aggccgactg aacgtgcccg
    14641 ggtgaaacgt gtgacttacc ggctgccggc tgccataatt tagcgccggc cggcagatga
    14701 tccccttctg ccgggtgccg gtcggtgcac aggctcgaaa gggcgtgtgc gcggggagcg
    14761 ctttgcgtaa aagaggaaga aggagcgcga ttcgcggctt cttgcagggg ccctggcctg
    14821 aaggacgggc aacatggcgc tgaatcagaa aacgctcaag aactcgatcc gcgccaccgg
    14881 cgttggtctg catacgggag acaaggtctt tctgtccctg cgcccggccc ccgtggatac
    14941 gggaatcgtc tttgtgcgca ccgatctcga cggcgagccg aggctcccgg cgcgggtgga
    15001 gaacgtgggc gataccacca tggcgacgtc gctgaccaac ggcaacgtca gcgtttccac
    15061 ggtcgagcat ctcatgtcgg cgtttgccgg gctcgggatc gacaatgcct tcgtggacgt
    15121 cagcgccccg gagttgccga tcatggacgg cagcgccgcg ccgttcgtgt ttctcatcca
    15181 gtccgccggg gtagaggaac agaccgcgcc cagacgattc gttcgcataa agaaggcggt
    15241 ggcgttccgg cagggagacg ttgtcgcgga gctcaagccg ttcgacgggt tccgcgtttc
    15301 ctacaccctg gtctacgacc acccggtatt ccgcaagcac gaacgctcag ccacggtgga
    15361 tctttccaac actgcctacg tcaaggaggt gtcgcgggcg cgtacgttcg gttttctggc
    15421 cgattacgaa cagctgcgat ccatgaacct cgcgcgggga gggagcctcg acaatgcggt
    15481 ggtggtggac gactaccgca tcctcaacgg ggaggggctc cggcaggccg acgagttcgt
    15541 caagcacaag attctcgacg ccgtgggcga cctctacctg ctcggctgtg gcctgatcgg
    15601 cgagttctgc ggctacaaat caggccatgg atccaacaat gcgcttttgc gcaggttact
    15661 agctgacccc gaggctttcg agatcgttac cttcgcaaac gaagcggacg cgccgacggg
    15721 cttcgccgct cccgtggttg ctaattgatt cgcagactca ttggccaatt tgaccgacat
    15781 gtctcaaaat gaggctgggg tcgaggggat aatcgtcaca gccgagggca caaggaaccg
    15841 ggcgcctgtc agaattcgct gacgcgcacc tcgattcgct gagccactcg gaaatcggct
    15901 agatcgtgaa gtttgctcag cagttcaggc gcaaggaatc gcaaccgcgt agcggttgca
    15961 gcgtttcggc agaccacgat gatcgagccg ttgcggatgt cgctgacgcg acagtcgttc
    16021 tgcagcgaag cgggcagcag ggctaacagc ccggccgtcc aggactcttg attcgccgac
    16081 tgacgtagga gacgttggag aggagtaaat cggcgttggc tttcgctcaa cagaagatcg
    16141 actttgagat tcgccatggc cgggtctcga tcatgaacga ccgtgtcggc cgccctcggg
    16201 ctatggattt tggcccgggt cggttcgttt cgccaccgtt tcgggttgcg gtcagggg
//
LOCUS       DBNHQR010000628         2672 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_62518, whole genome shotgun sequence.
ACCESSION   DBNHQR010000628 DBNHQR010000000
VERSION     DBNHQR010000628.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_62518"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="AC16VR_14390"
     CDS             <1..749
                     /locus_tag="AC16VR_14390"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6012396.1"
                     /translation="MFDGDWRAVMLLWSAVALAAAVAWFAIASICGPRVPGFDQGPIV
                     STAGRPSSRGVIAELSRAPAVRLVLALAVGSFLINHGLNNWLPELLRSGGLGIVEAGY
                     WAALPMVVGIIGSLTIPRLATPERRFRILFALCAAAAIASAALTFGGDVVLTFALVLQ
                     GFVRASLMTILILTLMELPVVQERHAGTASGLFFSAAEIGGVLGPLGLGLMHDLTGGF
                     NAGLYGLAAIAAAMALGAMRLSGLAGARPG"
     gene            complement(746..2089)
                     /locus_tag="AC16VR_14395"
     CDS             complement(746..2089)
                     /locus_tag="AC16VR_14395"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6012397.1"
                     /translation="MTAGSGTLHSDMAWPRQGYAWYVVIILFVCSIFSFLDRQVIALM
                     VDDIKLDLGLNDFEIGLLQGPPFGIFYALMSIPIALAADRYSRRNIIIAGVAFWSAAT
                     AACGLAGNFWHLFLARVGVGSGEACLSPCAYSMISDYFRRQLLPLAMSIFTMGNLTGV
                     GLAMIIGGAVIGYAKSLDEIVIWGLGDVAPWQFAFIVIGLPGLLLAALIFTVREPLRR
                     GLSAPTAETDATSLRAFGGFVRQHWRTFATLFGSFTMLVLVAFGNFAWVPTFFIRTFG
                     WSEAYAGTIYGSIVAIFGTGGALFGGWFASYLMRRGYVDAPYRATLICSLPLAPLAVL
                     VFVVIPDGHWAAIVFAPYSFFSAVPAGHAATAMMTVTPNEMRAKISSAYLFFSNFIGL
                     SLGAATVGFLTHNVFGDDMMVGYSLTLVNGIGAPLAVIAIALGMKHYRRSVAELD"
     gene            2146..2592
                     /locus_tag="AC16VR_14400"
     CDS             2146..2592
                     /locus_tag="AC16VR_14400"
                     /inference="COORDINATES: protein motif:HMM:NF025050.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6012398.1"
                     /translation="MTMPDNTSITQMGYVVADLDAAMERWKTTVGAGPFERLNHVEVV
                     DGLYRGTPTNVDISVAIAEVGGIQLELIEQHNDVPSCYRDLFAAGEEGLHHVAVQPED
                     FDAEVARYEALGCPQAFGGLYEGARFVYMDTSGSLGIMVELVERRD"
BASE COUNT          464 a          872 c          923 g          413 t
ORIGIN      
        1 ccatgttcga cggcgactgg cgcgccgtga tgctgctgtg gagcgccgtg gcgctggcgg
       61 ccgccgttgc gtggttcgcc atcgcatcca tttgcggtcc ccgcgtgcct ggcttcgatc
      121 agggccccat cgtctccacg gccggtcggc ccagcagccg cggtgtcatc gcggagctct
      181 cccgcgcgcc ggccgtgcgg ctggtgcttg cactggcggt gggttcgttc ctcatcaacc
      241 acggtctcaa caactggctg cccgagctgc tgcgcagcgg gggcctgggc atcgtggagg
      301 ccggctactg ggcggcgctt cccatggtgg taggcatcat cggctccctg accatacccc
      361 ggctggctac ccccgagcgc cgcttccgca ttctgttcgc cctgtgcgcg gcggccgcca
      421 tcgcaagcgc ggcactcacc ttcggcggcg acgtggtgct gaccttcgcc ctggtgctgc
      481 aggggttcgt gcgcgcctcg ctgatgacca tcctgatcct gacgctgatg gagctgccgg
      541 tggtgcagga gcgccatgcg gggacggcga gcgggctgtt cttctcggca gccgagatcg
      601 gcggcgtgct cggcccgctg gggctcggcc tgatgcacga cctcaccggc gggttcaacg
      661 cggggctgta tgggctggcc gccatcgcgg ccgccatggc cctgggcgcg atgaggctct
      721 ccgggctcgc cggagccagg cccggctagt ccagctccgc gacgctgcgc cggtagtgct
      781 tcattccgag cgcgatggcg atcaccgcca gcggcgcgcc gatgccgtta acgagcgtca
      841 gggagtagcc caccatcatg tcgtcaccga acacgttgtg ggtgagaaag cccaccgtcg
      901 cagcaccaag cgaaagcccg atgaagttgc tgaagaacag gtaggccgac gagatcttgg
      961 cccgcatctc gttgggcgtc acggtcatca tggccgtggc cgcgtggccc gcggggaccg
     1021 cgctgaagaa cgaataagga gcgaagacga tggccgccca gtgcccgtcg ggaatgacca
     1081 cgaataccag caccgccagc ggcgccagag gcagcgagca gatgagggtc gcccggtaag
     1141 gcgcgtcaac gtagccgcgc cgcatcaggt agctggcaaa ccagccgccg aacagcgcgc
     1201 cgcccgtccc gaagatggcc acgatggagc cgtagatcgt cccggcgtag gcttcggacc
     1261 agccgaaggt gcggatgaag aacgtgggga cccaggcgaa gttgccgaag gccaccagca
     1321 ccagcatggt gaagctgccg aacagggtgg cgaacgtgcg ccagtgctgg cgaacgaacc
     1381 ctccgaaggc tctgagcgaa gtggcgtccg tctccgccgt cggcgccgac aggccgcggc
     1441 gcagcggctc gcggacggtg aagatgagcg cagcgagcag cagccccggc aggccgatga
     1501 cgatgaaggc aaactgccag ggtgccacgt cgccgagacc ccagatcacg atctcgtcca
     1561 ggctcttggc atagccgatc accgccccgc cgatgatcat ggcgagcccg acgccggtga
     1621 ggttgcccat ggtgaagatg ctcatggcca ggggcagaag ctgacgccgg aaatagtcgc
     1681 tgatcatgga gtacgcgcac ggtgacaggc acgcttctcc gctgcccacg ccgacccggg
     1741 cgagaaacag atgccagaag ttgccggcaa ggccgcaggc ggcggtggcc gcgctccaga
     1801 acgccacgcc ggcgatgatg atgttgcgcc ggctgtagcg gtcggcggcc agcgcaatgg
     1861 gaatggacat cagcgcgtag aaaatgccga acggcggccc ctgcagcaac ccgatctcga
     1921 aatcgtttaa gcccaggtcc agcttgatgt cgtccaccat cagcgcgatg acctgccgat
     1981 cgaggaagga gaatatcgag cagacgaaca ggatgatgac cacgtaccag gcgtagccct
     2041 gccgcggcca ggccatgtcg gagtgaagcg ttccggagcc ggcggtcatg gtgatggggg
     2101 tccggacaag gttatcctta actctcgctg cgcaggggac agccgatgac tatgccagat
     2161 aacaccagca ttacccagat gggctacgtg gtcgccgatc tcgacgctgc catggagcgc
     2221 tggaagacca ccgtcggcgc aggcccgttc gagcgcctga atcacgtcga ggtcgtcgac
     2281 ggcctgtacc ggggaacgcc cacgaacgtg gacatctccg tggccatcgc cgaggtgggc
     2341 ggcatccagc tcgagctcat cgagcagcac aacgacgtgc cctcctgcta ccgggatctc
     2401 tttgccgcgg gcgaggaggg cctgcaccac gtggccgtgc agcccgagga tttcgacgcg
     2461 gaagtggcgc gctacgaggc gctcggctgc ccccaggcct tcggcggcct ctacgaaggc
     2521 gcccgcttcg tctacatgga cacgtccggc tcgctgggca tcatggtcga gctcgtggag
     2581 aggcgggact gacgcgccaa ccgggctggt tcagagcccg gcgttcggat tgatcagata
     2641 cttctggccc gtggctttcc gaccgtaggc gg
//
LOCUS       DBNHQR010000629         3431 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_62521, whole genome shotgun sequence.
ACCESSION   DBNHQR010000629 DBNHQR010000000
VERSION     DBNHQR010000629.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_62521"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="AC16VR_14405"
     CDS             complement(<1..631)
                     /locus_tag="AC16VR_14405"
                     /inference="COORDINATES: protein motif:HMM:NF014839.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HIR6012399.1"
                     /translation="MVSITLPDGSQRQFDGSVTGTELARDISKSLAKAAVAVRVDGEL
                     KDIYIPIEQDAAVEVITRDSQAGVDLLRHDAAHVLAEAVKELYPETQVTIGPTIENGF
                     YYDFSRAEPFTPEDLETIEARMREIVARNEEIRREVWDRDEAVAYFQSIGESYKAEII
                     AGIPEGEEVGLYRQGEFLDLCRGPHLESTGRLGGAFKLMSVAGAYWRGDS"
     gene            complement(702..2288)
                     /locus_tag="AC16VR_14410"
     CDS             complement(702..2288)
                     /locus_tag="AC16VR_14410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012935.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HIR6012400.1"
                     /translation="MDAYDYAIVGAGSAGCVLANRLSADPETRVLLIEAGPGNGNPLL
                     HIPAGWATNFRNPKVDWGFSTEPEPALNGRSIYWPRGKVLGGSSAINGMIYIRGVPLD
                     FDRWAQSGARGWSYEEVLPYFRKAERQQRVADEFHGAEGPLHVQDVRDRRAAMDAFID
                     GMTELGIPRNDDFNGQEQAGCGYYQFNQLRGRRWSTATAYLGPARRRPNLDVMTGVLV
                     RRVCFDGKAATGLEIERARSPATRVAAREVILASGAINSPQLLELSGVGDGERLRGLG
                     IEVVGDLPEVGENLQDHLLTKTVYGTHPEQSINREVQGWRVGVAALKWLMLRRGPLTT
                     GSAPVGGFWYTRDGLDAPDVQVHFASGATLYNEEGRIRAMPVPAMTAVVNQSRPESVG
                     SIHITSADPRQPPAIRPNYLDSELDRDTLVRGLGMIIDIFESTAMRPYVTERLLPEPG
                     VTSDEALLEYIRGDAATTYHPTSTCRIGSVVDENLKVLGVSGLRVVDASVMPFVVSGN
                     TNAATIMIAEKAADLIRAGG"
     gene            complement(2327..2830)
                     /locus_tag="AC16VR_14415"
     CDS             complement(2327..2830)
                     /locus_tag="AC16VR_14415"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR6012401.1"
                     /translation="MTLQATFNTSKGAIQVSLMPERAPVTVANFANLARRGFYDGLKF
                     HRVIADFMVQGGCPLGTGTGGPGYRFEDEFDSEVRHDRPGRLSMANAGPGTNGSQFFI
                     THVPTPWLDGNHTIFGEVQGPEDQAVVDSIAQGDVIESVVIEGDLDALLEAHADRIEE
                     WNRALDR"
     gene            3019..>3431
                     /locus_tag="AC16VR_14420"
     CDS             3019..>3431
                     /locus_tag="AC16VR_14420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR6012402.1"
                     /translation="MRLLLVTDAWFPQVNGVVRTLSTTMENLARLGHETEVIAPARFR
                     SVPCPTYPEIRLALCTPGRVGNMIAEFAPDAVHVATEGPLGLAARWWLKRRGIPFTTS
                     FHTMFPQYVRLRFGLPEAWTFAAMRRFHGAATQTM"
BASE COUNT          588 a         1149 c         1104 g          590 t
ORIGIN      
        1 gggagtcgcc ccgccagtaa gcgccggcca ccgacatgag cttgaaagca ccgccaagcc
       61 ggcccgtgga ctccaggtgc gggccccggc agagatccag gaattcgccc tggcggtaca
      121 gaccgacctc ctcgccctcg ggaatgccgg caatgatctc cgctttgtag gactcgccga
      181 tggactggaa gtaagccacg gcttcgtccc ggtcccagac ctcgcggcgg atctcctcgt
      241 tgcgcgcgac gatctcccgc atgcgggcct cgatggtctc cagatcctct ggcgtgaacg
      301 gctccgcccg ggaaaagtcg tagtaaaagc cgttttcgat ggtgggcccg atggtgacct
      361 gggtctccgg gtagagttcc ttgaccgcct ccgccagcac gtgggccgcg tcgtgacgca
      421 gaaggtccac accggcctgg gagtctcgag tgatgacctc caccgccgcg tcctgctcga
      481 tggggatgta gatgtccttg agctcaccgt ccacgcgaac ggccaccgcc gccttggcca
      541 gcgacttgga gatatccctg gccagctccg tgccggtcac cgacccgtcg aactggcgct
      601 gggaaccgtc gggcagggtg atggatacca ttagaaaaga cctggtggcc gaaaaacggc
      661 gattttgcca aacggccccg ccgcggtcga cgtgtaattg atcagccgcc tgcccgaatg
      721 aggtctgcgg ccttctccgc gatcatgatg gtcgccgcat tggtattgcc cgaaaccacg
      781 aaaggcatga cggacgcatc cacgacccga agcccggaga cccccagaac cttcaggttc
      841 tcatccacca cggatccgat gcggcacgtg ctcgtgggat ggtaggtggt ggcagcgtcg
      901 ccgcggatgt actcgagcag cgcctcgtca gaggtgacgc cgggttcggg caggagacgc
      961 tcggttacgt acggccgcat cgccgtgctc tcgaagatgt cgataatcat tcccagaccc
     1021 ctgacgaggg tatcccggtc gagctcgctg tcgagatagt tgggccggat ggccggcggt
     1081 tgcctcgggt cggcgctggt gatgtggatc gagcccacgg actcaggccg actctggttg
     1141 accaccgcgg tcatggcggg caccggcatg gcccggatac gcccttcctc gttgtaaagc
     1201 gttgcgccgg aagcgaaatg aacctgcacg tcgggggcgt cgaggccgtc acgcgtgtac
     1261 cagaagccgc cgacaggagc cgatccggtg gtcaacggtc cacgcctgag catgagccat
     1321 ttcaaggcgg caacgccgac ccgccacccc tgcacctccc ggttgatcga ttgctcggga
     1381 tgagtcccgt aaaccgtctt ggtcagaagg tgatcctgga ggttctcgcc gacctcgggc
     1441 aggtcaccga cgacctcgat gccgagcccc cgcagccgct cgccgtcccc gactccggac
     1501 agctcgagga gctgcggcga gttgatcgcg ccgctggcca ggatgacttc cctggcagct
     1561 acccgcgtgg cgggggatcg ggcccgctcg atctccaggc ccgtggccgc cttcccgtcg
     1621 aagcataccc gcctgacgag gacgcccgtc atcacgtcga ggttgggccg gcgtctggcg
     1681 ggaccgaggt aggcggttgc cgtgctccag cgccgacccc ggagctgatt gaactggtag
     1741 taaccgcaac cggcctgctc ctgcccgttg aaatcgtcgt tgcgcggaat gcccagctcc
     1801 gtcatgccgt cgatgaacgc atccatggcc gctcgcctgt cgcgcacgtc ctggacgtgc
     1861 aggggtccct ccgcgccgtg aaactcgtcg gccacccgct gctggcgctc ggccttacgg
     1921 aaatacggaa gcacctcctc gtagctccag ccccgagccc ccgactgggc ccagcggtcg
     1981 aagtccaggg gaacgccgcg aatgtagatc atcccgttga tggcgctgga gccacccaga
     2041 accttgcccc gaggccagta gatggagcgc ccgttcaggg ccggctcggg ctcagtggaa
     2101 aaaccccagt ccaccttcgg gttgcggaag ttggtcgccc accccgcagg aatgtgcagc
     2161 agcgggttcc cgttgcccgg ccccgcctcg atcaggagca cgcgcgtctc cgggtccgcc
     2221 gacaaccggt tggccagaac gcagccggcc gagccggctc ccacgatggc gtagtcgtag
     2281 gcgtccatga ccggtttcgc gcttcagggg ctcgtgacgg ggcggatcaa cggtccaggg
     2341 cccggttcca ttcctctatg cgatccgcgt gggcttctag cagagcgtcc aggtccccct
     2401 cgatcaccac gctttcaatg acgtcgccct gggcgatgct gtccaccacg gcctgatctt
     2461 ccggcccctg aacctcgccg aagatggtgt ggttgccgtc gagccaggga gtgggtacgt
     2521 gggtgatgaa gaactgcgag ccgttggtgc cggggccggc gttggccatg gatagccgac
     2581 cggggcgatc gtggcggacc tccgagtcga actcgtcctc gaagcggtag ccgggaccgc
     2641 cggtccccgt acccagcgga cagcctccct gaaccatgaa gtcggcgatc acgcgatgga
     2701 atttcagacc atcgtaaaaa ccccgccggg caaggttcgc gaagttcgct acggttaccg
     2761 gagcccgctc gggcatcagg gaaacctgaa tggctccttt ggaagtgttg aaggtggctt
     2821 gcagagtcac gttagctcct gtggggtaac ttgaagaggc tcgagagccg tatcggcggc
     2881 cagtgaaaca caacgggcag gcgttttcga gcggcgccac ctgcccaggc ggccaaaccc
     2941 ccagcgggtt gtagccgccc tcccagtccc gctactatcc tcgtcaagcg accgcaccgc
     3001 ggcgattaga gagataacgt gcggctactg ttagtcacag atgcctggtt cccacaggtg
     3061 aacggcgtgg ttcgaacgct gtccaccacc atggaaaatc tcgcgcggct gggccacgag
     3121 accgaggtga tcgcccctgc gcgcttcaga agcgtcccct gtccgaccta cccggagatc
     3181 cggctcgcgc tgtgcactcc gggccgggtc gggaacatga tcgccgagtt cgcgccggac
     3241 gcggtgcacg tcgccacgga agggccactg gggctggccg cccgctggtg gctcaagcgc
     3301 cgcggcatac cgttcaccac ctcgttccac accatgttcc cgcagtacgt caggctccgg
     3361 ttcgggctgc ctgaagcatg gacgtttgcg gccatgcgcc gcttccacgg ggccgccact
     3421 cagaccatgt a
//
LOCUS       DBNHQR010000630         2606 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_884980, whole genome shotgun sequence.
ACCESSION   DBNHQR010000630 DBNHQR010000000
VERSION     DBNHQR010000630.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_884980"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..476)
                     /locus_tag="AC16VR_14425"
     CDS             complement(195..476)
                     /locus_tag="AC16VR_14425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012403.1"
                     /translation="MAVQVTFVEIAQHSATQTVTFSEAELPGASSKPQISADLQQTTS
                     GSKPLSLLVEGWGDPPDSSGANACVPSDPMGTACGRRVASDPIVTISYH"
     gene            747..1103
                     /locus_tag="AC16VR_14430"
     CDS             747..1103
                     /locus_tag="AC16VR_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012404.1"
                     /translation="MHVGEGQRHDQAMIAPRGLEEETLVQMIRGVEGYTALLSKNRGT
                     MDDGCYETLSRALGGIIESLSVLTTGPRGRLDGDRLREKLEAIAGQELSKRAREEAGS
                     PRSAQAHLRLLEKTGA"
     gene            complement(1114..>2606)
                     /locus_tag="AC16VR_14435"
     CDS             complement(1114..>2606)
                     /locus_tag="AC16VR_14435"
                     /inference="COORDINATES: protein motif:HMM:NF013188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:proton antiporter domain-containing
                     protein"
                     /protein_id="HIR6012405.1"
                     /translation="FERLWGMRRLVFGMGSAQIVITAIVIGAVAWGFGNSIEASVLLG
                     GCLALSSTAIVMQLLMEQGRFGTQVGRASFAILLAQDIAVVPILFLVSAFGASQGGSL
                     GLDFGFAVGQALLAILVILGVGRVLVRPLFRFVAQTDSPELFMAVTLLTIIATAAGTH
                     AAGLSAALGAFLAGLLLAECEYRQEIEINMEPFKGLLLGLFFMSVGMGIDLAEIFTRP
                     VWIVLSVAGLFVIKALVTANVARGFGIAWRHAVEMGLLLGQGGEFAFVVVGAALALGL
                     LPDATAQFMLIVVGITMFLSPLVAKVARSAGDLLRERATDDVDEEIDLAADLGGHVII
                     VGYGRTGQLLASMLDRQMVPHAALDLDRDRVAALRSGGAPVFLGDACRPAMLSKLRLK
                     HAAALAICTDDPGATERILTSARSAVGRVPIIARARDNVHAAELLGLGATRVVPELLE
                     SGLQMGHVMMEEIGLPGTVARDLVEALRSEAERGLSAAADSLETST"
BASE COUNT          556 a          853 c          778 g          419 t
ORIGIN      
        1 catctcaaag gctttattat tctggatcct attgttcacg acgtcctcct cgtcctattg
       61 cacaggtcct tctggcacgg catccacggg cattgacaac gcccagctgc agtatgctcc
      121 gcaaatgctg cgggttccga gaagccagtc tcagatcgac gctttatccc gccgaggccc
      181 ggcaagggaa aggttcagtg gtaggaaatc gtcacgatcg gatcgctggc cacccgtcgg
      241 ccgcatgcag tacccatggg atcgctcggc acacaggcgt tggcaccaga tgaatccgga
      301 gggtcgcccc agccctcgac gagaaggctg agcggcttgc ttccagacgt cgtctgctga
      361 agatcggcgg agatttgcgg cttggaggat gctcccggca gctcagcttc ggagaaggtc
      421 acggtctggg tcgcgctgtg ctgcgcgatt tctacgaagg tgacctgcac cgccaccgtc
      481 cgggaacctg ccagagcttc gcaaccgaac gcgaaccaca gaaacagcaa gaggaaaact
      541 cgacacccag gtgccatcga ttatctttcc agccaacgac ctacgcccac ccgtttgagt
      601 ctaccctcga cggccttccg acttgtaaag aaatgtgcac gaaaacaggg gccttcttca
      661 gaatcggtca atgattcctc aaccactagt acaagcttct tgtgcaccat acgttcgggc
      721 acttcaacaa ccgggggaca ccggcaatgc atgtgggtga ggggcaacga cacgaccagg
      781 ccatgatcgc gccgcgaggg ctcgaggaag aaaccctggt gcagatgatc cgtggggtcg
      841 aaggctatac cgcgctgcta tccaaaaacc ggggcaccat ggacgacgga tgctacgaga
      901 cgctgtctcg agccctcggc gggatcatcg aatcgctatc cgttctgacg accgggccgc
      961 ggggccggct cgatggtgac cggttgcgag agaagctgga ggctatcgcc ggtcaggagc
     1021 tgtcaaaacg cgcccgggag gaggccggct ccccgcgttc tgctcaggca caccttaggt
     1081 tgctcgagaa gacgggcgcc tgagcgcccg gcttcaggtc gaagtttcca acgaatcggc
     1141 cgcagcgctc aggccacgct cggcttcgct tcgcagcgcc tccacgagat cccgggcaac
     1201 ggtgcccgga agcccgatct cttccatcat gacgtggccc atctgcagcc cggactcgag
     1261 gagctcgggg accacccggg tagcccctag tcccagtagc tcggcggcgt gaacgttgtc
     1321 acgggctcgg gcgatgatgg gcacgcgccc caccgcgctc cgggcgctgg tcagaatcct
     1381 ttccgtagcg cccggatcgt ccgtgcagat tgccaacgcg gccgcatgct tcaaacgtag
     1441 cttgctcaac atcgccgggc ggcaggcgtc accgaggaaa acgggcgctc ccccagaccg
     1501 cagtgctgcc acccggtccc gatccagatc caacgcggcg tgagggacca tctgacgatc
     1561 gagcatggat gccaggagct gccccgttcg accatagccg acgatgatga catggccgcc
     1621 caggtcggcc gccagatcaa tctcttcgtc gacgtcatca gtagcccttt cccgaaggag
     1681 atccccggca cttcgggcca ccttggcaac gaggggcgaa aggaacatgg tgatgcccac
     1741 cacgatgagc atgaactggg cggtggcatc tggcagcagg ccgagcgcca gggccgctcc
     1801 gaccaccacg aaggcgaatt caccgccctg cccgagcagc agccccatct ccaccgcgtg
     1861 ccgccaagca atgccgaacc cgcgcgcaac gttggccgtg accagcgctt tgatgacgaa
     1921 aaggcctgcg acggagagaa cgatccacac cggccgggta aagatctcgg ccagatcgat
     1981 tcccattccc accgacatga agaacagccc gagcaacaag cccttgaacg gttccatgtt
     2041 gatttcgatt tcctgacggt actcacactc tgcgaggagc agacccgcca ggaacgcgcc
     2101 cagggctgcc gaaagccccg cggcgtgagt tccggctgcc gtcgcgatga tcgtcagcag
     2161 agtgaccgcc atgaagagtt cgggactgtc cgtttgagcg acgaaacgga agagcggccg
     2221 cacgaggacg cgccctaccc cgagaatcac gagaatcgca agcagcgctt gtccaaccgc
     2281 aaagccgaag tcgagcccca gggagccgcc ctggcttgcc ccgaaggcgc tgaccagaaa
     2341 caggatggga accaccgcga tgtcctgggc cagcagaatc gcaaaactgg cgcgtcctac
     2401 ctgcgtcccg aaccttccct gctccatgag cagctgcatg acgatggccg tggacgatag
     2461 ggcgaggcac ccgccaagga gcacggatgc ctcaatggag ttaccgaagc cccaggcaac
     2521 ggcgccgatc acgatggccg tgatgacgat ctgagcggat cccatcccga aaaccagccg
     2581 ccgcattccc cacagccgct cgaagg
//
LOCUS       DBNHQR010000631         2368 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1454603, whole genome shotgun sequence.
ACCESSION   DBNHQR010000631 DBNHQR010000000
VERSION     DBNHQR010000631.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1454603"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="AC16VR_14440"
     CDS             complement(<1..583)
                     /locus_tag="AC16VR_14440"
                     /inference="COORDINATES: protein motif:HMM:NF024785.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HIR6012406.1"
                     /translation="MVQSIRGMRDVLPEEGRRWRVVEDAIRAVLTTHAYEEVHLPLLE
                     ATELFSRGVGEATDIVEKEMYTLADRDGDSISLRPEGTAVCARALLQHGLLHNQTQRV
                     FYAGPMFRYERPQKGRYRQFYQIGAEAFGLAGPDVDAELILMCQIMWKALGISGETRL
                     EINTLGSGAARAAYRAALVGFLTPLAAGLDADSQ"
     gene            complement(586..1569)
                     /locus_tag="AC16VR_14445"
     CDS             complement(586..1569)
                     /locus_tag="AC16VR_14445"
                     /inference="COORDINATES: protein motif:HMM:NF024855.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HIR6012407.1"
                     /translation="MSEEEREPKGPGATLREARERMQISTRQVAEALNLPVHTIEAIE
                     AEDPERLPAPVFARGYTRAYARLLELDADAIVAQYPQGEDNVEQVHLIREPRSSASVI
                     RFGLAALLWILGLGVVAVVVWVVWQWSGKDTELAEPVTEARGLLEEVADQPVPSDVGT
                     DPSFSGVPQQEDAALTGREAAEIAGFSAEPEPEPESVAQREVITPRRPGTRPETEPTP
                     SDAGAMATQAAPVEDLAALREAGRRITSAGDDVLRMEFFEDCWVEVMSSTGSLLYGDL
                     NRSGRVLVLVGEAPFRVLLGYAPGVRMSFNGEAVVLTPHTRNAVADLVLGQ"
     gene            complement(1595..>2368)
                     /gene="pilW"
                     /locus_tag="AC16VR_14450"
     CDS             complement(1595..>2368)
                     /gene="pilW"
                     /locus_tag="AC16VR_14450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HIR6012408.1"
                     /translation="PRACTGGGMRLGALAILWSVLLSGCVTETTGGLPEPAVAEKRVQ
                     AQLDLARGYLENRDYSRARAPLNRALEIDPRSVEGHVLMAVLLQGEGEMALAEQHYKL
                     ALRHEPNDSQALNNYASFLYRQQRYDEALGLLRELVKDTEYPARSQAYENLGLAELIA
                     GDREAARQAFIRALNLNFAQPRSSLELARLSYSDGNFELAHEYYEAFRVRARQSPRSL
                     CLGMKISEALGNSDQTASYGMALQNLYPESAEADECEVP"
BASE COUNT          403 a          819 c          734 g          412 t
ORIGIN      
        1 gctggctgtc agcatccaga ccggccgcca gcggcgtgag aaagcccacc agagctgccc
       61 gataagcggc ccgcgcagcc cccgatccga gggtattgat ctccaggcgg gtttcgccgg
      121 agattcccag agccttccac atgatctggc acatcaagat cagctctgca tccacgtccg
      181 gccccgccaa cccgaaagcc tcggcgccaa tctggtagaa ctgccgatag cggcccttct
      241 gcggacgttc gtagcgaaac atggggcctg catagaaaac gcgctgggtc tggttgtgga
      301 gcagaccgtg ctggagcaac gcgcgggcgc agacggccgt gccctcgggg cgcagagaga
      361 tgctgtctcc gtcccggtcg gcaagggtgt acatctcctt ctcgacgatg tctgtggcct
      421 cgcccacgcc acggctgaac agctccgtgg cctcgagcag gggcaggtga acctcttcgt
      481 aggcgtgcgt ggtcaacacc gcgcggatgg cgtcttccac gacccgccag cgcctgccct
      541 cttcgggcag cacgtcgcgc atgcctctga tcgactggac catcgctact gaccaagcac
      601 gaggtccgca acggcgttgc gggtgtgggg cgtgagcacc acggcctcgc cgttgaaact
      661 catgcgcacg ccgggcgcgt acccgagcag cacgcggaaa ggcgcttctc ccaccagcac
      721 gaggacgcgt ccggatcgat tgagatcgcc gtagaggaga cttccagtgg aagacatgac
      781 ttccacccag caatcctcga aaaactccat gcgaagcacg tcgtcacctg cggacgtgat
      841 ccgccgacca gcctcccgga gcgcagccag gtcttcgacc ggggccgcct gagtcgccat
      901 ggcgccggca tctgacggcg tcggttcggt ttccggcctc gtccccggcc gccgcggcgt
      961 gatcacctct cgctgcgcca cagactccgg ctccggttcg ggctccgctg aaaacccggc
     1021 gatttccgcc gcttccctgc ccgtaagagc ggcgtcttcc tgctgcggga cgcctgagaa
     1081 tgaggggtcg gtgccgacgt cggacggtac cggctggtcc gcgacctctt cgagcaggcc
     1141 cctcgcttcg gtcacgggtt ccgcaagctc cgtatcttta ccgctccact gccagacgac
     1201 ccagacgacg accgcgacga cgcccagtcc cagaatccag agcagcgctg cgaggccgaa
     1261 tcggattacc gatgcgctgc tccgaggctc gcgaatcaag tgaacctgct cgacgttgtc
     1321 ttcgccctgg ggatactgcg ccacgatggc gtccgcatcg agctcgagaa gccgcgcgta
     1381 ggcacgcgtg tagccgcggg caaagaccgg cgccggcaat cgctccggat cttcggcctc
     1441 gatcgcctcg atggtgtgaa cgggcaggtt cagggcttcc gccacctgcc gggtggagat
     1501 ctgcatgcgc tcgcgggctt cgcgaagcgt cgcgccgggc cccttcggct cgcgctcttc
     1561 ctcgctcaag tcgtcacccc ggctcggccc atcatcaggg cacctcgcac tcgtccgctt
     1621 cggcagattc gggatagagg ttctgcaggg ccatcccata gctcgccgtc tggtcgctgt
     1681 tgcctagcgc ctcgctgatc ttcatgccca gacacaggct gcgcggcgac tggcgggcac
     1741 gcacccggaa ggcctcgtag tattcgtggg caagctcgaa gttcccatcg gaataggaca
     1801 accgggcaag ctccaggctc gagcggggct gagcgaaatt caggttcagc gcgcggatga
     1861 acgcctgccg cgccgcctca cggtctccgg ctatcaactc ggcgaggccc aggttttcgt
     1921 aggcctgaga gcgcgccgga tactctgtgt ctttgacgag ctcgcgaagc agcccgagcg
     1981 cttcatcgta acgctgctga cggtagagaa agctcgcata gttgttcagg gcctgagagt
     2041 cgttcggctc gtggcgcaga gcgagcttat aatgctgctc ggccagcgcc atctcgccct
     2101 caccctgcaa gagcacggcc atcaggacat gaccttccac cgatcgcggg tcgatctcca
     2161 gcgcgcgatt cagcggtgcc cgggctcggc tgtaatcgcg gttttccagg tagccacgag
     2221 ccaggtcgag ctgcgcctga acgcgttttt cggcaaccgc cggttctggc agcccacccg
     2281 tggtttccgt cacgcagccg ctgagcagca ccgaccacaa gatcgccaac gcccctagtc
     2341 tcatgccacc tcctgtgcac gcacgcgg
//
LOCUS       DBNHQR010000632         4399 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_315748, whole genome shotgun sequence.
ACCESSION   DBNHQR010000632 DBNHQR010000000
VERSION     DBNHQR010000632.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4399)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_315748"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..1765
                     /locus_tag="AC16VR_14455"
     CDS             200..1765
                     /locus_tag="AC16VR_14455"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007348244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HIR6012409.1"
                     /translation="MAVDIESTAKHTTHLNLAAAALAELAVLRGEGQFASNGAIVVRT
                     GHRTGRSPRDRFIVEEPSTADAIDWGTINQPIAQDVFDALWDRVQIHLEDRERFVSNL
                     HVGADPEHYLPIEVTTEYAWHALFGRALFIKPENYNPHNKSVWNIANAPSFVCDPERD
                     GTASDGTVMINFAQRKVLLAGMRYAGEMKKSMFSVLNFLLPEKDVLPMHCSANMGHDG
                     AVTLFFGLSGTGKTTLSADPDRLLIGDDEHGWGKGTVFNFEGGCYAKCIDLTRENEPL
                     IYDAIRFGAIVENVIIDQDTREPDYTDGSLTENTRACDPRSNIEAKVTDNRGGEPGQV
                     VFLTCDVSGVLPPVSLLSKEAAAYHFLSGYTAQVGSTEVGSTEAYKATFSTCFGAPFF
                     PRAAGVYADLLIKRIEEFGSKVFLVNTGWTGGGYGVGKRFAIPDTRAIIRAIQSGALD
                     DAETRRLPGFNLDIPTSVPGVDAGLLNPRDTWSDKAAYDESARSLIAKFAENFEKFSV
                     AQAIVDAGPTTDY"
     gene            1805..3376
                     /gene="gshA"
                     /locus_tag="AC16VR_14460"
     CDS             1805..3376
                     /gene="gshA"
                     /locus_tag="AC16VR_14460"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HIR6012410.1"
                     /translation="MAHAAAPIDPALLDALRDQPASLREIQRGIEKESLRVTPEGSLS
                     DRPHPAALGSALTHPNITTDFSEAQLELITEVHQSPEATLAQLEGVHRFVYQNLDGEL
                     LWSASMPCVLGTDEEIPVGRYGGSNIARAKTIYRLGLGNRYGRLMQTISGIHYNFSVP
                     QTFWKVLAESRGKEPTQEFVTEAYFGLIRNFRRFSWLLIYLFGSSPAICKSFVKNQPH
                     ELASFDEGSLYLPHATSLRMGRLGYQSDAQRSLHISYNGLEQYAASMHTALTRPYPPY
                     EAIGVKVDGEYRQLNASLLQIENEFYGTIRPKRRIVSGERPLNALRSRGVEYVEVRCL
                     DLNPFLHMGIDTVEIRFIDAFLLYCLLADSPEDSERESELMGRNQLAVVERGREPDLQ
                     LEQLRGTITRKAWAEDVLHACRPLAELLDEATGSDLYRHALSEQSSKNRQPALTPSAR
                     VLAVMQTQGIPFFRFAMNQSIAHKGYFDEHPMRGEQLAEHQATSQQSLARQQEIEAAD
                     SVDFDVFLKDYLALE"
     gene            3373..4014
                     /locus_tag="AC16VR_14465"
     CDS             3373..4014
                     /locus_tag="AC16VR_14465"
                     /EC_number="3.1.4.14"
                     /inference="COORDINATES: protein motif:HMM:NF016239.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein phosphodiesterase"
                     /protein_id="HIR6012411.1"
                     /translation="MNFLAHCLIAAQSTDPEEPDLVAGGFLGDFVKGPIGPELPPGLA
                     LGVRLHRRIDAFSNRHPAIQGSCNRFAGPLRRLAPVLVDIIADHCLAKHWEQFSNEPI
                     AAFTARTYDRIASHERWLPDHGHRFLSYMRDEDLLAGYARFEAMHRALCSVTRRLDRE
                     HLNADMLSATEANLPGLEADFLGYFPELVAHARQWLRDEGPPPGPQTPGTVQR"
     gene            4087..>4399
                     /locus_tag="AC16VR_14470"
     CDS             4087..>4399
                     /locus_tag="AC16VR_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017341396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR6012412.1"
                     /translation="MKAVLCKAFGPPEDLVMEEVANPEPGPGQILVDIHASALNFPDV
                     LMIAGKYQSLPPFPFSPGGEIAGTVAAVGDGATRFKVGDRVFGGTGHGGFAEQIALPE
                     MA"
BASE COUNT          840 a         1475 c         1338 g          746 t
ORIGIN      
        1 cgacggcgag gtcacggtga cctgcgagat ctgcggcacc gtctacgcct acgaccccat
       61 cgacattcac ctgctgctgc aaagggaagc cccgcagctt cactgagcga gcttccctta
      121 caatcccttt cattccgaat aggccaaatc tcaccgctct ggcataatcg atcgattgtc
      181 aggggaagac gctgaaagca tggcagtcga catagagtca acggcaaagc acaccactca
      241 tctcaacctt gccgccgccg cgcttgccga actggccgta ttgagggggg agggtcaatt
      301 cgccagcaac ggcgccatcg tcgttcgaac cggtcatcgc accgggcgct cgccgaggga
      361 tcggttcatc gtggaggagc cgtcgacggc cgacgccatc gactggggaa ccataaacca
      421 gcccatcgcg caggacgtat tcgacgcgct ctgggaccgg gtgcagattc acctggagga
      481 ccgcgaacgg ttcgtttcca acctccacgt cggggcggac ccggaacact atctgcccat
      541 cgaggtgact acggagtacg cgtggcacgc gctgttcggg cgggcactgt tcatcaagcc
      601 ggagaactac aacccccaca acaagtccgt gtggaacatc gccaacgcac cgagtttcgt
      661 ctgtgacccc gagcgcgacg ggacggccag cgacgggacg gtgatgatca actttgctca
      721 gcgcaaggtg ctgctcgccg ggatgcgcta cgcgggcgaa atgaaaaaat ccatgttctc
      781 ggtgctcaac ttcctcctcc cggagaagga cgtcctgccc atgcactgct cggcaaacat
      841 gggccacgac ggtgcggtga cgctgttctt cggcctgtcc ggcaccggca agacgaccct
      901 gtcggcggat cccgaccggc tgctcatcgg cgacgacgag cacgggtggg gcaaaggaac
      961 cgtcttcaac ttcgagggcg gctgctacgc caagtgcatc gacctgaccc gggagaatga
     1021 gccgctcatc tacgatgcga tccggttcgg cgccatcgtc gagaacgtca tcatcgacca
     1081 ggacacccgc gaacccgact acacggacgg atcgctgacc gagaacacca gggcctgtga
     1141 tccgcgcagc aacatcgaag ccaaggtgac cgacaaccgt ggcggcgaac ccggccaggt
     1201 ggtatttctc acctgtgacg tgagcggcgt gctgccgccg gtttccctgc tctccaagga
     1261 agctgccgcc taccacttcc tgtccggcta cacggctcag gtggggtcca cggaagtggg
     1321 ctctacggaa gcctacaagg caaccttctc cacctgcttc ggggcgccct tcttcccgcg
     1381 tgcggccggt gtgtacgccg atctgctgat caagcggatc gaggagttcg gctccaaggt
     1441 gttcctggtc aacacgggct ggaccggcgg cggctacggc gttggcaaac gcttcgccat
     1501 cccggacacg cgggccatca tccgggccat tcagtccggc gccctggacg acgccgagac
     1561 ccggcgcctg ccaggtttca acctggacat tccgacatcg gtgccgggcg tggacgcagg
     1621 tcttctcaac ccgagagata cgtggtccga caaggccgct tacgacgaat ccgcgcgcag
     1681 cctgatcgca aaattcgcag agaacttcga aaagttcagc gtcgctcagg ccatcgttga
     1741 tgctggtccg accacggatt attgaggtag cctgaaggcc gccccgttgg gctaagaggc
     1801 ggatatggcc cacgcagccg cacccatcga cccggcgctc ctggacgcat tgcgcgacca
     1861 gccggcttcc ctgcgagaga tccagcgggg catcgagaag gaaagcctgc gggttacgcc
     1921 ggaaggctcg ctctcagacc gaccgcatcc ggccgccctg ggctccgcgc tcacccatcc
     1981 gaacatcacc acggacttca gcgaggctca gctggagctg atcaccgaag tgcatcagtc
     2041 tcctgaggcg acgctcgccc agctggaggg cgttcaccgg ttcgtctacc agaacctgga
     2101 cggcgagctg ctctggtccg ccagcatgcc gtgtgtgctc ggcacggacg aggagattcc
     2161 cgtaggtcgc tacgggggct cgaacatcgc cagggccaag acgatttacc ggctggggct
     2221 cggtaatcgc tacgggcggc tgatgcagac catctcaggc atccactaca acttttcggt
     2281 cccgcagacg ttctggaagg tgctggcgga aagcaggggg aaagagccga cgcaggaatt
     2341 cgtaacggaa gcgtatttcg gcctcatccg caatttccgg cgcttttcgt ggctgctcat
     2401 ctacctgttc gggtcgtcgc ctgcgatctg caaatcgttc gtgaaaaatc agccccatga
     2461 gctggcgtcc ttcgacgaag gctcgctcta ccttccacac gccacgtcgc tgcgaatggg
     2521 gcgcctgggc taccagagcg acgctcagcg cagcctccac atttcctaca acggtctcga
     2581 gcagtacgcg gcatccatgc acacggcgct tacccgacct taccccccgt acgaggccat
     2641 cggcgtcaaa gtcgacggcg agtaccgaca gctgaacgcc tcgctgctgc agatcgagaa
     2701 cgagttctac ggaaccatcc ggccgaaacg ccgcatcgtg agcggcgaaa ggcccctgaa
     2761 cgccctgaga tcccgcgggg tcgagtacgt cgaggtcaga tgcctggacc tgaacccgtt
     2821 tctccacatg ggcatagaca ccgtcgagat ccgcttcatc gacgcgtttc tgctttactg
     2881 cctgctggcg gacagccctg aggacagcga gcgggaatcg gagttgatgg ggagaaacca
     2941 gctcgccgtg gtcgagcggg gccgcgagcc ggaccttcaa ttggagcagc tgcgcggcac
     3001 catcacacga aaggcatggg cggaagacgt cctgcacgcc tgccgtccgc tcgccgagct
     3061 tctggacgag gccaccggga gcgacctgta ccgccacgcg ctcagcgagc agtccagcaa
     3121 gaaccggcag ccggcgctga cgccgtcggc gcgggtgctg gccgtcatgc agacccaggg
     3181 cataccgttt ttccgcttcg ccatgaacca gtccattgcc cacaagggtt acttcgacga
     3241 gcacccgatg cgaggtgagc agctggccga gcaccaggcc acctcgcaac agtccctggc
     3301 gcggcagcag gagatcgagg ccgccgacag cgtggatttc gacgtcttcc tcaaggacta
     3361 cctggctctc gagtgaactt tctcgcccac tgcctgatcg ctgcgcaatc caccgacccc
     3421 gaggaaccgg acctcgtcgc cggcggcttt ctgggcgact tcgtcaaggg ccccattggc
     3481 cccgaactgc ccccgggcct ggctctcggc gtacggctgc atcggcgcat cgacgccttc
     3541 agcaaccggc acccggccat ccaggggagc tgcaatcgat ttgcgggccc cctgcgccgg
     3601 ctggcgcccg tgctcgtcga catcatcgcc gaccactgcc tggccaagca ctgggagcag
     3661 ttcagcaacg agcccatagc agccttcacc gcaaggacct acgatcgcat cgcgtcccat
     3721 gagcgctggc tccccgacca cggccaccgc ttcctcagct acatgcgcga cgaagacctg
     3781 ctggcggggt acgcccggtt cgaagccatg caccgggctc tgtgctcggt gacacgtcgc
     3841 ctggatcgcg agcacctcaa cgccgacatg ctcagcgcta ccgaagcgaa cctgcccggg
     3901 ctggaggccg atttcctggg gtacttcccg gaactggtgg cccatgcacg gcagtggttg
     3961 cgcgacgagg gcccgccccc cggaccccaa acgccgggca cggtgcagcg ctgaaaccgg
     4021 tactgccagg ttgacgctgc cgaaccgacc gttactcttg cgttctttga ttttcaggaa
     4081 gcatccatga aagcggttct gtgcaaggca ttcggcccac cggaagacct ggtgatggag
     4141 gaggtggcaa atccggagcc tgggcccggc cagatcctcg tcgacattca tgcctccgcc
     4201 ctgaacttcc cggacgtgct gatgatcgcc ggcaagtacc agagtctccc accgttccct
     4261 ttctctccgg gcggcgagat cgcgggaacc gttgccgcgg tcggcgacgg cgccacgcgt
     4321 ttcaaggtcg gcgaccgggt ctttggaggg accggccacg gcggcttcgc cgagcagatc
     4381 gccctgcccg agatggcca
//
LOCUS       DBNHQR010000633         2636 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1708151, whole genome shotgun sequence.
ACCESSION   DBNHQR010000633 DBNHQR010000000
VERSION     DBNHQR010000633.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1708151"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="AC16VR_14475"
     CDS             <1..256
                     /locus_tag="AC16VR_14475"
                     /inference="COORDINATES: protein motif:HMM:NF014868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012413.1"
                     /translation="RDTGRSEARPPAFVSAEGLKPTEHLAMQAAVQPHVDHAISKTVN
                     VPADSSFEEFEEIYAEAHRLGLKGCTTYRPGGSRAAVLKH"
     gene            352..1695
                     /locus_tag="AC16VR_14480"
     CDS             352..1695
                     /locus_tag="AC16VR_14480"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6012414.1"
                     /translation="MAEETSKHPTPPKTQREFMQKTNREMAAEWAIDVAQDPYSLPIE
                     SLNPAHPALFEANAMLPYFERLRAEAPVHYCEHSQFGPYWSITRFEDVKYVDTHEKLF
                     SSDIRNGGIRLGQQPDPEPDPMFHLPMFIMEDPPKHDEQRKVIAPMFTARHLAGLADL
                     IRERAGRILDGLPVGETFNWVREVSVELTGQMLATLFDVPQEDRHKLIHWSDTVERIG
                     DPDYFETPEQGFQELWKCWEYFDAVWKERQAQSEPGSDLISMLVHGESTKDMPPNEYL
                     GNILLLIVGGNDTTRNSITGGVLALNQNPAEYEKLLADPGLIPNMVPEIIRWQSPVAH
                     MCRTALEDTEVGGRTIRKGDKVAMWYLSGNRDEDVIDDANRFIIDRANARQHLSFGFG
                     IHRCVGNRLGEMQLGIIWEEILKRFARVEVVGEPQYLRSSFIRGIRELPVRVHPA"
     gene            complement(1700..2386)
                     /locus_tag="AC16VR_14485"
     CDS             complement(1700..2386)
                     /locus_tag="AC16VR_14485"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024809.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6012415.1"
                     /translation="MAADLLIYGTLGSPFVRKVLVTLQEKGLEWELEEVDVFDPPDWF
                     VALSPARRIPVMRDRSIAAEGVAGTIADSSAICAYVERKHPERPLYPADAYDYARALW
                     FEEYADTLVAARIGFGLLRTLVAAPARGQPPDLERARKAYHEDLPPIFTYLEDVIGDR
                     PFLVGDRFSIADIALASQLAGMRVTRVPIDGGRWPNLADYADRLLARPSFVDRLAEVA
                     SRMPAEDFVP"
BASE COUNT          519 a          871 c          833 g          413 t
ORIGIN      
        1 gcgcgacacc ggcaggagcg aggcgcggcc gccggcgttc gtgagcgccg aggggctcaa
       61 acccaccgag catctggcca tgcaggcggc ggtccaaccc cacgtggatc acgccatctc
      121 aaagaccgtc aacgtgcccg cagactcgag cttcgaggag ttcgaggaga tctacgccga
      181 ggcgcaccgc ctgggcctga aggggtgcac cacctatcgg cccggcggct cccgggcggc
      241 ggtgctcaag cattgaaccg aggagcgcgg actgcttaaa aaaattcgag cgcgcttgcc
      301 gtcgatcctg ccgttacaat gacggtttgt gatcaggcag cgtggacgat catggccgaa
      361 gaaaccagca agcaccccac cccgccgaaa acccagcggg agttcatgca gaagaccaat
      421 cgggagatgg cggccgagtg ggccatcgac gtcgctcagg acccctactc cctgccaatc
      481 gaatcgctga acccggcgca tccggccctg ttcgaggcca acgccatgct gccctacttc
      541 gagcggttgc gcgcggaagc gcccgtgcat tactgcgagc acagtcagtt cggcccctac
      601 tggtccatca cccggtttga ggacgtgaag tacgtggata cccacgagaa actcttctcg
      661 tcggacatac gcaacggcgg gatccgcctg ggccagcagc cggacccgga gccggacccc
      721 atgttccatc tgcccatgtt catcatggag gatccgccga agcacgacga gcagcgcaaa
      781 gtgatagcac ccatgttcac ggcgcggcat ctggccgggc tggcggatct gatccgtgag
      841 cgtgccggcc gcatcctgga cgggctgccg gtgggggaga ccttcaactg ggtccgcgaa
      901 gtttccgtgg aactcaccgg gcagatgctc gcaaccctct tcgacgtccc ccaggaggat
      961 cgccacaagc tcatacactg gtcagacacc gtcgagcgca tcggcgatcc ggactacttc
     1021 gagacccccg agcagggctt tcaggagctg tggaagtgct gggagtactt cgatgccgtc
     1081 tggaaggagc gccaggccca gagcgagccg ggcagcgatc tgatctccat gctcgttcac
     1141 ggggaatcca cgaaggacat gccacccaac gagtacctgg gcaacatcct gctgctcatc
     1201 gtcggcggca acgacaccac ccgcaactcc atcaccggcg gcgtgcttgc gctgaaccag
     1261 aacccggccg agtacgagaa gctgctcgcc gatcccgggc tgataccgaa catggtcccg
     1321 gagatcatcc gctggcagtc gccagtggcc cacatgtgcc ggacggctct ggaagacacg
     1381 gaagtcggcg gcaggaccat ccgcaagggc gacaaggtgg ccatgtggta cctctccggt
     1441 aaccgggacg aagatgtcat cgacgatgcc aatcgcttca tcatcgaccg ggccaacgcg
     1501 cgtcagcact tgtccttcgg cttcggcatc caccgctgcg tgggcaaccg gctcggcgag
     1561 atgcagctgg gcatcatctg ggaggagata ctcaagcgct tcgccagggt agaggtggta
     1621 ggcgagcctc agtatctccg ctcgagcttc atccgcggca ttcgcgagct gcccgtgcgc
     1681 gtgcacccgg cctgatcgct cagggcacga agtcctccgc gggcatacgc gaagcaacct
     1741 ctgccagccg gtctacgaag ctcggccggg cgagcagacg gtcggcgtag tcggccagat
     1801 tgggccagcg accaccgtcg atgggcaccc gcgttaccct catgcccgcc agctgcgaag
     1861 ccagggcgat gtcggcaata gagaagcggt cgcccaccag gaacggccga tcgccgatga
     1921 cgtcttcgag ataggtgaag atcggcggca ggtcctcgtg gtaggccttg cgcgcccgct
     1981 cgagatccgg cggctgcccc cttgccgggg ccgccaccag cgtgcgcagc agcccgaacc
     2041 cgatgcgcgc cgcgaccagg gtgtccgcgt actcctcgaa ccacaacgcc cttgcgtagt
     2101 cgtaagcatc tgccgggtat agggggcgct cgggatgttt gcgctccacg taggcacaga
     2161 tggcggagga gtccgcgatg gtgccggcga ccccttcggc ggcgatgctc cgatcccgca
     2221 tcacgggaat gcgccgggcg gggctgagag cgacaaacca gtctggcggg tcgaagacgt
     2281 cgacctcctc cagctcccac tcgaggccct tttcctgcag cgtgaccagc accttgcgaa
     2341 cgaaggggga tcccaacgtt ccgtagatga gcagatcggc ggccattctc ccttccccgc
     2401 ttcgtcccga tgagcaaaga gtggccgctg gaccgtggtt gtgcaagcgc tcggctgcgc
     2461 tggaactttc cgggccgcaa agtttaagct cgccctccag cgggcgcgca tccgcccgat
     2521 aatgaccatt ccaggagggg acgaccatga aacacatcgt tgcggtgctc accgagccca
     2581 ccgagggcaa ggaagacgaa ttcaacgact actacgagaa cctgcacctg gacgaa
//
LOCUS       DBNHQR010000634         1690 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1646229, whole genome shotgun sequence.
ACCESSION   DBNHQR010000634 DBNHQR010000000
VERSION     DBNHQR010000634.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1646229"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..1248
                     /locus_tag="AC16VR_14490"
     CDS             79..1248
                     /locus_tag="AC16VR_14490"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6012416.1"
                     /translation="MTWSPFAPEVMADPANGHRELLDRCPVHHCEDFDPPFFTLSRYA
                     DVEEALRDIETFSSHYGQGPRFTEPQGMLCDPPQHTYMRKLVQQAFTPKAMEALRIQV
                     ADLTEKLIDDILAGEPEFDLHDDFAFPLPVIIIAGMLGVPTEDLDRFKHWSDVQVAAM
                     GSSDPTRFEADQAAFFEYMVNHYRKRHAAIVAGEAVPDDLLTMIAGARADDGSFIAEA
                     DAQSMLTQLLVGGNETTTSLITNMVWRLLEDRERWQEVVADPVLVEAAVEESLRFDPP
                     VLGLYRNTTRDVVLHDTVIPTGSKVYINYAAANRDASVFERPDEFDLHREAKRHMSFG
                     LGVHFCLGAPMARLEAEIALKALARRMPDLKLLGPGQRIAPFFLWGRRNLPVAWR"
     gene            1258..>1690
                     /locus_tag="AC16VR_14495"
     CDS             1258..>1690
                     /locus_tag="AC16VR_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012417.1"
                     /translation="MSEALSPSPRPTLAGWIIRDVLITSGLAAMLLGLAAWHEAEGSL
                     AAALVAPALGFVAAYVLCYVYHEWGHLIGARLTGAAMPLAPYAGALIGHFDIQAHSRR
                     QFLWLSWGGVIAYVALMSIALSVYLHGGLGPAGAGFAVGGLA"
BASE COUNT          266 a          575 c          531 g          318 t
ORIGIN      
        1 ggtgtccaac tggcgcatgc ggtgtccaaa taggcaaacg ccgatatact ggcgagcccg
       61 cacttcagga aggtcgatat gacctggagt cccttcgcgc ccgaggtcat ggccgacccg
      121 gccaatggcc atcgggagct tctcgaccga tgccccgtcc atcattgcga ggattttgat
      181 cccccgttct tcacgctttc ccgctatgcc gacgtggagg aagcgctgcg ggacatcgag
      241 acgttttcca gccactacgg ccagggcccg cggttcacgg aacctcaggg catgctctgc
      301 gatccgcctc agcacaccta catgcgcaag ctggtccagc aggccttcac gcccaaggcc
      361 atggaagcgc ttcgtattca ggtcgcagat ctcaccgaaa agctcatcga cgacatcctt
      421 gccggggagc cggaattcga cctgcacgat gatttcgcct tccccctgcc ggtgatcatc
      481 atcgccggca tgctgggggt gcccaccgaa gacctcgatc ggttcaagca ctggtccgac
      541 gtccaggtag cggccatggg atcgtcggat cccacgcgtt ttgaggcgga tcaggccgcc
      601 ttcttcgagt acatggtcaa ccactatcgc aagcgccacg cggccatcgt ggccggggaa
      661 gcggtaccgg atgatctgct gacgatgatt gccggcgctc gcgccgacga cggctcgttc
      721 atcgcggagg cggatgcgca atccatgctc acccagctcc tcgtgggcgg gaacgaaacc
      781 accacgtcgc tcatcaccaa catggtctgg cggctcctcg aggaccggga gcgctggcag
      841 gaagtggtgg cagaccccgt cctggtcgag gccgccgtgg aggagagcct gcgtttcgac
      901 ccgcccgtgc tcggcctcta tcgcaacacc acccgtgatg tcgtgcttca cgacaccgtg
      961 ataccgacag gcagcaaggt ctacatcaac tacgccgccg ccaaccgcga tgcctctgtc
     1021 ttcgagcgtc cggacgagtt cgacctgcat cgggaagcca agcggcacat gtcgttcggg
     1081 ctcggcgtgc acttctgcct cggcgccccc atggcgaggc tcgaagcgga gatcgcgctc
     1141 aaggcgctgg cgcggcgtat gcctgatctg aagctcctcg ggccgggtca acgcatagcg
     1201 ccattcttcc tgtggggccg gcgcaacctg ccggttgcct ggcgttagtc cggcgcggtg
     1261 tccgaagccc tctcgccatc gccgcggccg acgcttgccg gctggataat tcgcgacgtc
     1321 ctcatcacct cagggctcgc cgcgatgctg ctggggttgg cggcctggca cgaggcggaa
     1381 ggttcgctgg ctgctgcgct cgtcgctccg gccctcgggt tcgtagcggc ttacgtgctc
     1441 tgctacgtct atcacgagtg ggggcacctg atcggcgccc ggctgaccgg cgctgccatg
     1501 ccgctggcgc cctacgccgg cgccctcatc ggccacttcg acatccaggc gcacagccgc
     1561 cgccagttcc tctggctgag ctggggcggg gtgatcgcct atgttgccct gatgagcatt
     1621 gccctctccg tctatctgca tggtgggctc ggccccgcgg gcgcgggttt tgccgtgggc
     1681 gggctcgcct
//
LOCUS       DBNHQR010000635         4725 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_251879, whole genome shotgun sequence.
ACCESSION   DBNHQR010000635 DBNHQR010000000
VERSION     DBNHQR010000635.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4725)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4725)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4725
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_251879"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..597
                     /locus_tag="AC16VR_14500"
     CDS             187..597
                     /locus_tag="AC16VR_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR6012418.1"
                     /translation="MRRPFGSSNSESDDEQINLTPMLDVVFIMLIFFIVTATFIKEVG
                     LDVNQPDQDKPKTIDPDKKSIVVRITNRDRIIIAQRDVDWRSVRANIERLHAENPEAP
                     VVIQPHPESRTETMIHIMDSARLAGVYNVSLAQN"
     gene            631..1620
                     /gene="ltaE"
                     /locus_tag="AC16VR_14505"
     CDS             631..1620
                     /gene="ltaE"
                     /locus_tag="AC16VR_14505"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HIR6012419.1"
                     /translation="MRTVDLRSDTVTRPTPAMRDAIYAAEVGDDVYGDDPTVNALESL
                     AAQMLGKEAALFVTSGTQANLVALLTHCGRGDEYIAGATAHTYWYEAGGAAALGGIQP
                     QTLPFEPDGTLDLTAVAKLVKPDDVHFARTRLLCLENTQAGRAAGPAYFAAARTFCDE
                     RGLGLHLDGARLFNAAIATGCEAQDIARHCDSVSVCLSKGLGSPAGSLLLGSADFIRE
                     ARRWRKMLGGGLRQAGILAAGGIYALNHHVDRLADDHSRAARLAQAVNERYPDGAEAH
                     TNMVFLNLAEAEVDRLKDHLADRGIVIRGPRWVTHLDVSDEDVARVVDAIRAS"
     gene            complement(1641..2879)
                     /locus_tag="AC16VR_14510"
     CDS             complement(1641..2879)
                     /locus_tag="AC16VR_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HIR6012420.1"
                     /translation="MQAILGIGFLLGLAWILSESRRTVRWRPVMVGLVVQLGLAALLL
                     RVPVVAESLLVLNKLVYAVEAATGAGAGFVFGYLGGGDAPFTVSAQGAMYVFAFRVLP
                     QILVFSVLVALLWYWRVLPAVIRGFGYLLRRALDVGGAVGTAAASSVFLGMVEAPLVI
                     RAYLERLSRSELFTVMTCGMSTVAGSIMVLYASVLGDVITGALGHILAASVINVVGAV
                     VVSRIMIPADDATAEEDGASGLSYVSNMDAITRGTADGLRLAVNVGAMLVVLVSLVAL
                     VNQGLGAVTVGEGPLSLERMMGWVFSPVAWLIGIPWGEAQAAGALLGTKLILNELVAY
                     LQLAGLPEGSLSDSSRLVMTYALCGFANFGSLGILLGGLNTLVPERRKEILSIGPKTL
                     ISGTIVTCNTGAIVSLIGLF"
     gene            complement(2906..3598)
                     /locus_tag="AC16VR_14515"
     CDS             complement(2906..3598)
                     /locus_tag="AC16VR_14515"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:NF013786.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase family
                     protein"
                     /protein_id="HIR6012421.1"
                     /translation="MLHHNSGMQGEPGQALAAELDIRLPRMDGLSLRVAPVADFEHEL
                     HGPELEAVARAVPKRRQEFATGRHLARAAMRELGLPAAPVPRRADRSPVWPDGCLGSI
                     THTNALAGVAVARAGVVEGIGIDLEESHRVSAELHDKLFTPTELGRYVDAPPGWADLL
                     FSAKESVYKAVNPIVGKYIGFHEVEVDVTFAEQRFSARYVGEHAPNALLENGAGYFLF
                     AEQHVFSVFIIP"
     gene            3622..4245
                     /locus_tag="AC16VR_14520"
     CDS             3622..4245
                     /locus_tag="AC16VR_14520"
                     /inference="COORDINATES: protein motif:HMM:NF019544.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol phosphotransferase CPT family
                     protein"
                     /protein_id="HIR6012422.1"
                     /translation="MQTGKIIFLNGSSSAGKTTIAVTLQQLLEEPFQHIALDQFRDGM
                     PGRFRGLNSPEGTPGARGLNVVPVERDGEKMTEIRFGEVGEQILRGMRRAISAFAAAG
                     NNVIVDDLLFKPDYLHDYAKALADHDAWLIGVRCSLDVVNEREAQRPGRFPGTATSHF
                     HQVHEHGAQYDLEVDTTTTTPRACAELIIERLARPPEALERLRSARP"
     gene            4312..>4725
                     /locus_tag="AC16VR_14525"
     CDS             4312..>4725
                     /locus_tag="AC16VR_14525"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - histone deacetylase activity,
                     NAD-dependent [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family NAD-dependent protein deacylase"
                     /protein_id="HIR6012423.1"
                     /translation="MTENEDVDAFRELIEAAGNVVFFTGAGISTESGIPDFRSPGTGL
                     WNKIRPIEFQDFVASEDKRREYWQRHFTGERPMANAKPNKGHDGVQRLIASGKATAVI
                     TQNVDNLHQDSGIPEDQVIELHGNASYATCLACGTR"
BASE COUNT          921 a         1625 c         1478 g          701 t
ORIGIN      
        1 tcgaaggagc gggccacaac gccaacgtgg accagcctga ggctttcaac gcggccctta
       61 aggattttct ggcgtctttc tgctgaccag aatgcgcccg acgggggggc atctcaggcg
      121 ctaggcatgg cccgtgatcc tgagtaagat accgggcctt gcagtacgaa tctcgggggg
      181 agcgcggtgc gcagaccatt cggcagcagc aacagcgagt cagacgacga gcagatcaac
      241 ctcacgccca tgctggacgt ggtgttcatc atgctcatct tcttcatcgt cacggccacc
      301 ttcatcaagg aggtgggtct cgacgtgaac cagcccgacc aggacaagcc gaaaaccatc
      361 gaccccgaca agaagagcat cgtggtgcgg atcaccaacc gggaccgcat catcatcgct
      421 cagcgcgacg tggactggcg gtccgtgcgc gccaacatcg agcggctgca cgcggagaac
      481 ccggaagccc ccgtggtcat ccagccccat ccggaatccc gcacggaaac gatgatccac
      541 atcatggact ccgcgcgcct ggccggcgtg tacaacgtct ccctggcaca gaactgaccc
      601 cggcctccta gcatctagga ggcccgaccc atcaggaccg tcgatctccg cagcgacacc
      661 gttacccggc cgaccccggc catgcgcgac gccatctacg cagcggaagt aggcgacgac
      721 gtctacggcg acgatcccac cgtcaacgcc ctggagtccc tggccgcgca gatgctgggc
      781 aaggaggccg ccctcttcgt caccagcggg acccaggcca acctggtggc gctgctcacc
      841 cactgcgggc gcggcgacga gtacattgcc ggcgccacgg cccacaccta ctggtacgaa
      901 gccggcgggg ccgccgctct cggaggcatc cagccgcaaa cgctgccctt cgagccggac
      961 ggcaccctgg atctcacggc ggtagccaag ctggtgaagc ccgacgacgt ccacttcgcc
     1021 cggacgcgtc tgctctgcct ggagaacact caggccgggc gcgcagccgg tcccgcctac
     1081 ttcgccgcgg cccggacgtt ctgcgatgaa cgtgggctgg gtctgcacct cgacggcgcg
     1141 cggctgttca acgctgccat cgccaccggg tgcgaggctc aggacatcgc caggcactgc
     1201 gattcggtct ctgtctgcct gtccaaaggc ctcggctcgc cggcaggctc cctgctgctg
     1261 ggtagcgccg acttcattcg cgaagcaagg cgctggcgca agatgctggg aggcgggctt
     1321 cgccaggccg gcatcctcgc cgctgggggc atttacgcgc tcaatcacca cgtggatcgc
     1381 ctggccgacg accactcacg ggccgcgcgt ctggcgcagg ccgtcaacga gcgatacccc
     1441 gacggtgctg aagcacacac caacatggtg ttcctgaacc tcgccgaggc ggaagtggat
     1501 aggctcaagg accatctggc cgatcgtggc atcgtcatcc gcgggccgcg ctgggtgacc
     1561 catctcgacg tctccgacga ggacgtcgcg cgcgtcgtcg acgccattcg ggcaagctga
     1621 ggcgccttgg tcttaccgct tcagaacagg ccgatgagcg acacgattgc cccggtgttg
     1681 caggtaacaa tcgtaccgga aatcaacgtt ttaggtccga tacttaatat ctctttgcgt
     1741 cgttccggaa cgagcgtatt caggcccccg agcagaatgc ccaggctgcc gaaattggca
     1801 aagccgcaca gcgcgtaggt catcaccagc cggctcgagt cgctcaagct tccctccggc
     1861 agcccggcga gctgcagata ggccaccagc tcgttcagga tcagcttggt gccgagcagc
     1921 gccccggccg cctgggcttc tccccaggga atcccgatca gccaggctac gggggagaac
     1981 acccatccca tcatccgctc cagagaaagc gggccctccc ccaccgtcac cgcgcccagg
     2041 ccctgattca ccagagcgac aaggctcacg agaaccacga gcatggcgcc gacgttcacg
     2101 gccagacgca gaccgtccgc cgtgccgcgc gtgatggcgt ccatgttgga gacgtagctg
     2161 agaccggaag cgccgtcctc ttctgccgtg gcgtcgtccg ccggaatcat gatgcgcgag
     2221 acgacgacgg cgccgaccac gttgatcacc gaagcggcca ggatgtggcc gagcgcgccc
     2281 gtaatgacgt cgccaagcac gctggcgtag aggaccatga tggatccggc cacggtggac
     2341 atgccgcagg tcatgaccgt gaagagctcc gaacgggaca agcgctcgag ataggcccgg
     2401 atcaccagcg gtgcttccac catgccgaga aacacgctgg aggcagccgc cgttcccacg
     2461 gcgccgccca catcgagtgc acgacggagc aggtagccga acccgcggat caccgccggc
     2521 agcacgcgcc agtaccacag cagcgccacg agcacgctga aaacgaggat ctgcggcaga
     2581 acgcgaaacg caaagacgta catggcccct tgagcgctta ccgtgaacgg cgcatcccca
     2641 ccgcccagat aaccgaacac gaaccccgcc ccggccccgg tcgccgcctc caccgcatag
     2701 acgagcttgt tgagcaccag cagagattcg gccaccacgg gaacccgcag cagcagcgcg
     2761 gcaaggccga gctgaacgac gagacccacc atcacgggcc gccagcgcac ggtgcgccgg
     2821 ctctcgctca gaatccacgc tagccccagc agaaagccga ttccgagtat ggcctgcatt
     2881 cagactctgc cttcgaggta ggggttcacg gaatgatgaa cacgctgaag acgtgctgct
     2941 cggcgaaaag gaagtagccc gcgccgtttt cgagcaaggc gttgggagcg tgctcgccca
     3001 cgtagcgggc agaaaagcgc tgctctgcga acgtcacatc cacctccacc tcgtgaaacc
     3061 cgatgtactt gccgacgatc gggttgaccg ccttgtagac ggattctttg gcgctgaaca
     3121 gaagatcggc ccaccccggc ggcgcatcca cgtaccggcc gagctctgtc ggggtgaaga
     3181 gcttgtcgtg cagctcggca gacacccggt gcgattcctc gagatcgatg ccgatgcctt
     3241 ccacgacgcc ggctcgggca acggcgacgc cggccagcgc attggtgtgg gtgatgctgc
     3301 caaggcaccc gtcgggccat acggggctgc ggtcggcccg gcgcgggacc ggggcggctg
     3361 gcaggccgag ctcgcgcatg gccgcacggg cgagatggcg gccggtggcg aattcctgcc
     3421 gccgctttgg cacggcccgg gcaacagcct ctagctccgg gccatggagc tcgtgctcga
     3481 aatccgccac gggggcgacg cgcaggctca ggccatccat ccgcggtagg cggatgtcga
     3541 gctcggccgc caacgcttgt ccaggctctc cctgcatgcc cgaattgtgg tgcagcatgg
     3601 gtgcagatac catcgccgcg tatgcagacc ggcaagatca tctttctgaa cggctcttcg
     3661 agcgctggta agaccaccat cgccgtcacg ctccagcagc tgctcgagga gccctttcag
     3721 cacatcgccc tggaccagtt ccgcgacggg atgccgggcc gtttccgcgg gctgaattcc
     3781 cccgagggaa cgcccggggc ccggggcctg aacgtcgttc cggtagagcg cgacggcgag
     3841 aagatgaccg agatccgctt cggcgaggtg ggtgagcaga tcctgcgcgg catgcgccga
     3901 gcgatctccg cctttgccgc cgctggtaac aacgtgatcg ttgacgatct gctcttcaag
     3961 cccgactacc tgcacgacta cgcgaaggcc ctggcagatc acgacgcctg gctgataggc
     4021 gtgcgctgca gcctcgacgt ggtcaacgaa cgcgaagcgc agcgccccgg gcgctttccc
     4081 ggcaccgcca cctcgcactt tcaccaggtg cacgagcacg gcgcgcagta cgacctggag
     4141 gtcgacacca cgacgacgac cccgcgcgcg tgcgcggagc tcatcatcga acgcctggcc
     4201 cggccgccgg aagcgctcga gcgactgcgc tcggccagac cctgatcctg ggacatcggc
     4261 aatcgacaac gcggggctga acacgcgaca accccacagg gaggcaggcc aatgaccgag
     4321 aacgaggatg tcgacgcgtt tcgcgagctg atcgaagccg ctgggaacgt ggtgttcttc
     4381 acgggcgcgg gtatcagcac cgagtcagga attcccgatt ttcgcagccc gggcaccggc
     4441 ctgtggaaca agatcagacc catcgagttt caggacttcg ttgccagcga agacaaacgc
     4501 cgggagtact ggcaacgcca cttcaccggc gagaggccca tggccaacgc caaaccgaac
     4561 aagggccacg acggggttca gcggctgatc gcctcgggca aggcgaccgc cgtgatcacc
     4621 cagaacgtgg acaatctgca tcaggactcg ggcattccgg aggatcaggt catcgagctg
     4681 cacggcaacg ccagctacgc cacctgcctc gcctgcggaa cccgt
//
LOCUS       DBNHQR010000636         4857 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_948816, whole genome shotgun sequence.
ACCESSION   DBNHQR010000636 DBNHQR010000000
VERSION     DBNHQR010000636.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4857)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_948816"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..164)
                     /locus_tag="AC16VR_14530"
     CDS             complement(<1..164)
                     /locus_tag="AC16VR_14530"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6012424.1"
                     /translation="MPDLAIGYVNVFVSDFDTALAFYRDKLGLEVAMADEEFGYASFD
                     TKGVGVAIAAV"
     gene            complement(274..1128)
                     /locus_tag="AC16VR_14535"
     CDS             complement(274..1128)
                     /locus_tag="AC16VR_14535"
                     /inference="COORDINATES: protein motif:HMM:NF025679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HIR6012425.1"
                     /translation="MTVGLTDAPVDGVMAVHVEFRGITLKPQSGPAIDFALSEKVQLL
                     DLQNGSVFPLLDAQTVDAGRYNWIELHTNAEIDGILDSYVVESKSNGMVELEVPSGTV
                     RLVSGFVVTAGKSNAVLLDWDVRKGLTDPEGLVSPSPDAKDAYKLTPALRLIDMTQYG
                     SLTGSVADALIMAESCTSNAEGDGNLVYVYRNHDTTPDDLGSFGEPVTTAPVRSNPEK
                     AGAYTYTVHYLDPGDYTVAFTCQGLDDDPATDAAEGAAVPDIEFSGQVNASVAEGEGT
                     TNEAPPID"
     gene            1541..2050
                     /locus_tag="AC16VR_14540"
     CDS             1541..2050
                     /locus_tag="AC16VR_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012426.1"
                     /translation="MAGNLGLIAGLILVGVQITDSNRIASAELFGSSVGSVISINTEQ
                     LGDSPGEAMSRVLYDPAAAETSDLYIADRIYDALLRQLVRVHVFDAMGLYGDDELVSP
                     AGFVGLHFEFFACPYGLAWLDQQIELFDTRGNEELAILTSLKMLRELAGARPLVLVCS
                     ATDKRTSAR"
     gene            2115..3344
                     /locus_tag="AC16VR_14545"
     CDS             2115..3344
                     /locus_tag="AC16VR_14545"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450 family protein"
                     /protein_id="HIR6012427.1"
                     /translation="MIDLNRPVNLASPEFAKDKYEWYERIREERPVFQTKISVLTVYT
                     VSRYEDCTNVLKDPNVLRNRTTATGGSRFPFPMPKSVQPLVQSMIQEDDPNHRRLREL
                     VRRAFRPQAIEQLESQIDAYSHELLDGLARRDSFDLQADYARPIPIRMIGDMMGLSRE
                     AVPQFQKAIRVLSEGFNGLKVLRTLFLDLPGAVRFVRALLDEKRSMPGDDVLTGLIEA
                     EDDGDRLTDDELVAMVFLLIVGGFETTLHLITNGVVTLLEHPEQLQRLKEDPGLIDSA
                     VEEILRHRGPVHGTKPGYAREDITLHGMTIPKGKPIMPLLAAANHDPRVFEKPQTFDI
                     ARSPNRHLGFGHGVHFCLGAHLARAEARVALRNLFERFPDLQLAVDPASLKLQAMPGW
                     HRYDGLPVIAGKMRSAA"
     gene            complement(3351..>4857)
                     /locus_tag="AC16VR_14550"
     CDS             complement(3351..>4857)
                     /locus_tag="AC16VR_14550"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012935.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HIR6012428.1"
                     /translation="PLIGIPKGIGKLVRSPDHAWFFPVEQPRQPDLPASEVWVRGKVV
                     GGSSSINGMIYSRGHPEDYEEWMALGGPAWGWAEMKAAYRSIEDHELGSADHRGAGGL
                     VHVSTGKLRYPAAEAMIEAGREMGLERKDDLNDEMLEGVGYYAHNIRRGRRQSAARTF
                     LKAARRRPNLHVQSQAHVDRVCFDGNRAVAVACRVAGDRRVFEARGEIILSAGTILSP
                     KILQLSGIGPADHLRDHGIEVVRDVPDVGARMREHLGFSMPHKLKGTKGLNHRLRGLG
                     LVGSALQYVATRGGPLATGPFEVGAFVRAVPGANRPDTQLYLGAFNYARSNDNFPVQL
                     ASPDSKPGMTIYGQLLNLTSEGTVRLRSADPDDPPVIVPNWLSTDYDRAAAVAMVRYM
                     RRYVRQPALARYVGDELIPGPTCQTDEEILNAVRRLSTSGLHGVATCRMGRDEGAVVD
                     GRLRVRGVEGLRVADCSVMPSLVSGNTNAPAMALGWRASDLIEEDAAGSRR"
BASE COUNT          879 a         1640 c         1502 g          836 t
ORIGIN      
        1 accgcggcaa tggcgacccc gacgcccttg gtatcgaagg acgcgtagcc gaactcctca
       61 tcggccatcg cgacctcgag acccagcttg tcccgataga aggccagcgc cgtatcgaaa
      121 tcactgacaa agacgttcac gtaccctatg gccagatcag gcattctgct ctcccgaacg
      181 ccacgacaag accatacgat accaccactg cggggtgcgc gagcgcttac atgaccaatg
      241 ccgtgccgga catcgaattc agcgggcaaa cgatcaatcg atagggggcg cttcgttggt
      301 cgtcccttcg ccctcagcaa ccgacgcgtt gacctgaccg ctgaattcga tgtccggcac
      361 ggcggcgcct tcagccgcgt ccgtggcggg atcgtcatcg agtccctgac aggtaaaggc
      421 aaccgtgtag tcgccaggat ccaggtagtg caccgtgtag gtatatgcac ccgccttctc
      481 cgggttggac cggaccggcg ccgtggtcac gggctcgccg aagctgccga gatcatcggg
      541 cgtcgtgtcg tgatttcggt aaacatacac caggttgccg tcgccttcgg cgttgctcgt
      601 gcaggactca gccatgatca aagcatccgc cacgctgccg gtcaggctgc cgtactgggt
      661 catgtcaatc agtcgcagcg ccggggtgag cttgtacgca tccttggcgt ctggactcgg
      721 gctcaccagc ccctccggat cggtcagccc tttgcggacg tcccagtcga gaagcaccgc
      781 gttgctcttg ccggcggtca ccacaaaacc gctcaccaga cgtaccgtgc cgctcggcac
      841 ctccagctcg accatgccgt tggatttgct ctccaccacg tacgaatcca gaatcccgtc
      901 gatctccgcg ttggtgtgca gttcgatcca gttgtagcgg cccgcgtcga cggtctgggc
      961 atccagcaac ggaaacacgc tgccgttctg caggtcgagg agctgaacct tctcggagag
     1021 cgcaaagtcg atggcaggcc cgctctgggg cttgagcgtg atgccacgga attcaacgtg
     1081 aaccgccatc acgccgtcga cgggggcatc cgtcaggccg acggtcaacg tgccggtgga
     1141 tggaccctca tcggatccgc tggatccccc acatcccaac attgaccagg tcaacgccgc
     1201 aaagacaatc gaacggtacg cagacataac tccttctcct gttgtccttc gaccacgcgg
     1261 ctgtttccac ccgtgaacga gacctaagca ggtatgacgc atcgggtcgc cgatcggctg
     1321 cgacgcatgc gtcgatctgc caaatggttc acccccgagg cgttagcggc ttcacgctcg
     1381 ggtggctgta accgagaaag tcatgcggtg ctgataagcg gtgcagagcc gcgcaagcca
     1441 gggtcgaaac gagaaacggc cgacttctgc taacctcgat ccaatcacaa tctccgggag
     1501 aaccattgtg gcgcgcgcaa agttgttgga gcttttgcag gtggcaggca atctggggct
     1561 gatcgccggg ctgattctgg tcggcgttca gatcaccgac tcgaaccgca tcgcctcagc
     1621 ggagctcttc ggtagcagcg tcggctccgt catctcaatc aacaccgagc agctcggcga
     1681 ctcacctggt gaggcgatgt cacgcgtgct gtacgaccct gctgccgcgg aaacctcgga
     1741 cctctacatc gccgaccgaa tttacgacgc gctgctgcgc cagctggtcc gtgtgcacgt
     1801 attcgacgcc atggggcttt acggtgacga cgagctcgtc agccccgcgg ggttcgtagg
     1861 ccttcacttc gagttcttcg cctgtcccta tggcctcgcc tggctcgacc agcaaatcga
     1921 gctgtttgac acccgcggca acgaggagct ggcaattctc acgagcctga agatgctgcg
     1981 ggagctcgcg ggcgcacgcc ctttggtatt ggtatgctct gcaacagata aacgaacgtc
     2041 tgcccggtaa aggggcaaca gcggtccgat agatccggac gagcacccgc tgcgaggcgc
     2101 caggagtcca gcccatgatc gacctgaacc gcccggtgaa tctggcttcg ccggagttcg
     2161 cgaaggacaa gtacgaatgg tacgagcgca tccgcgagga gcgcccggtg ttccagacca
     2221 agatcagcgt gcttaccgtc tacaccgttt cccgctacga ggactgcacg aacgtcctca
     2281 aggacccgaa cgttctgcgc aaccgcacca cggccaccgg aggaagccgc ttccccttcc
     2341 ccatgccgaa gtccgtccag ccgctcgtgc agagcatgat ccaggaagac gacccgaatc
     2401 accggcgcct gcgcgagctg gtcagacggg ccttccggcc ccaagccatc gagcagctgg
     2461 agagccagat cgacgcctac tcccacgaac tgctcgacgg gctggcgcgc agggacagct
     2521 tcgatcttca ggccgactac gcgcgaccga tccccatccg catgatcggc gacatgatgg
     2581 ggctttcccg cgaggcggta ccgcaatttc agaaggcgat ccgagtgctc tcggagggct
     2641 tcaacgggct gaaggtgctg cgaacgctgt ttctggatct gcccggcgcc gttcgtttcg
     2701 tccgcgccct gctcgacgag aaacgctcga tgccgggcga cgacgtcctc accgggctca
     2761 tcgaagccga ggacgatggc gaccggctca ccgacgacga gctggtggcc atggtatttc
     2821 tgttgatcgt cggtgggttc gagacgacgc tgcatctcat caccaacggg gtcgtaacgc
     2881 tcctcgagca ccccgagcag ctgcagcggc tgaaggaaga tcccggcctg atcgattcgg
     2941 cggtggagga gatcctccga caccggggtc ccgtgcacgg caccaagccg ggctacgccc
     3001 gggaggacat cacgcttcat ggcatgacga ttccgaaggg gaagcccatc atgccgctgc
     3061 ttgccgccgc caatcacgac ccacgggtgt tcgagaaacc acagacattc gacatcgccc
     3121 gctcacccaa tcgtcacctc ggcttcggcc atggcgtgca cttctgcctg ggcgcacacc
     3181 tcgcccgggc cgaagcgcgg gtggcgctgc gcaacctatt cgagcgattt cccgacctgc
     3241 agctggccgt ggatccggcg tccctcaagc tgcaggccat gccgggctgg caccgctacg
     3301 acgggctgcc cgtgattgcc gggaaaatgc gctcagccgc ctagctgcga tcagcgacgc
     3361 gagcctgccg cgtcctcttc gatcaggtcg cttgcccgcc aacccagcgc catggccggg
     3421 gcattggtgt tgcccgagac cagggagggc atcacagagc aatcggcgac gcgcaaacct
     3481 tcgacgcccc tgacccgcaa ccggccgtcc accaccgcgc cttcgtcccg gcccatccgg
     3541 caggtggcca caccgtgcag gccgctggtg gaaagccgcc gcacggcgtt gaggatctcc
     3601 tcgtccgtct ggcaggtcgg cccggggatc agctcgtcgc cgacgtacct ggccagcgct
     3661 ggctggcgca cgtagcggcg catgtaacgg accatggcga ccgccgccgc gcgatcgtaa
     3721 tccgtgctga gccagttggg cacgatcacc ggcgggtcgt caggatcggc ggagcgcaga
     3781 cgcaccgtgc cttcgcttgt cagattcagg agctggccgt agatggtcat gcccggcttg
     3841 ctatcgggcg acgccagctg aactggaaag ttgtcgttgc tgcgcgcgta gttgaaggcg
     3901 ccgaggtaga gctgcgtgtc cggccggttg gcgccgggca cggcgcggac gaaggccccg
     3961 acctcgaagg gtccggtggc gagcgggccg ccgcgggtcg ccacgtactg cagcgcgctg
     4021 cccaccagac ccaggccgcg cagccgatgg ttcaggccct tggttccctt aagcttgtgc
     4081 ggcatcgaaa aacccagatg ctcgcgcatc cgcgcgccca cgtcgggaac gtcgcgcacc
     4141 acttcgatgc cgtgatcccg caggtggtcg gccgggccga tgccggagag ctgcagaatc
     4201 ttgggagaga ggatggtgcc ggccgagagg atgatctcgc cccgggcctc aaagacccgc
     4261 ctgtcacccg cgacgcgaca cgccaccgcc acggcccgat tgccatcgaa gcacacccga
     4321 tcgacgtggg cctgcgactg cacgtgcagg ttggggcgcc gccgggcggc cttcagaaaa
     4381 gtgcgcgccg cgctctgccg acgtccgcgg cggatgttgt gagcatagta gccaacaccc
     4441 tcgagcatct cgtcgttgag gtcgtccttg cgctccaggc ccatttcgcg gccggcctcg
     4501 atcatggcct cggctgcggg atagcgcagc ttgcccgtgc tgacgtgcac cagcccaccc
     4561 gctccgcggt ggtcggctga ccccagctcg tgatcctcga tggagcgata agccgccttc
     4621 atctccgccc agccccacgc cgggccgcca agcgccatcc actcctcgta gtcctcggga
     4681 tgcccgcggg agtagatcat gccgttgatg gagctggaac cgccgaccac cttgccccgc
     4741 acccacacct cggaggccgg caggtccggc tgccgcggct gctcgaccgg aaagaaccag
     4801 gcgtggtcgg ggctcctgac cagcttcccg atgcccttgg gtatgccgat gaggggg
//
LOCUS       DBNHQR010000637         5847 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1201483, whole genome shotgun sequence.
ACCESSION   DBNHQR010000637 DBNHQR010000000
VERSION     DBNHQR010000637.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5847)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5847)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5847
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1201483"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="AC16VR_14555"
     CDS             complement(<1..411)
                     /locus_tag="AC16VR_14555"
                     /inference="COORDINATES: protein motif:HMM:NF014769.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta/alpha barrel domain-containing protein"
                     /protein_id="HIR6012429.1"
                     /translation="MAPDGFPHADELADAVGESVRAALAEDVGTGDITAELIAPDARA
                     TAEVITRDAGIFCGAPWVLETCAQVDAAIEVTLHCADGDRVSPGQTLFALSGPARGLL
                     TAERTMLNFVQLLSGTATATRRYVDLIAGTAARLL"
     gene            498..1049
                     /gene="ampD"
                     /locus_tag="AC16VR_14560"
     CDS             498..1049
                     /gene="ampD"
                     /locus_tag="AC16VR_14560"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HIR6012430.1"
                     /translation="MRVRKDHCLVGVPFRASPNCDERPDPEDVSLIVIHGISLPPGRF
                     GGDLVDGLFTNSLPDELDPGLAELRDVRVSAHLLIDRAGRITQYVPFHRRAWHAGSSG
                     WCGRPSCNDYAIGIELEGTDELDYADAQYRALIAVSRALFDRYPRLSVDAVVGHQEIA
                     PERKTDPGPAFDWCRYLGALAGP"
     gene            1046..2020
                     /locus_tag="AC16VR_14565"
     CDS             1046..2020
                     /locus_tag="AC16VR_14565"
                     /inference="COORDINATES: protein motif:HMM:NF012692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR6012431.1"
                     /translation="MKRIGIDLGGTKIKGIVLAEDGRVLSTRRAATPSQSYAGTVAAI
                     VRMVAELDGDSGEARDLPVGIGTPGIWMAQRSAMKNCNSTWLNGQPLLLDLEHALGAR
                     VRIANDADCFALSEAMAGTGRGYGSVFGVILGTGVGGGFVTGGRLMSGPNGLAGEWGH
                     TPLPYLGLHEALEISVGSELDRRHEQANALESRLAVRTCYCGRPGCVETYLSGPGWRQ
                     THRELWSEDVPTETIAKRRDERSEQTFFLYTRMLARSLAQIVNIVDPAVIVLGGGLSN
                     VQALYQPVNEMLARYVFSGECDTQVKAPQLGAESGVLGAAQLWSDNGG"
     gene            2055..4718
                     /gene="aceE"
                     /locus_tag="AC16VR_14570"
     CDS             2055..4718
                     /gene="aceE"
                     /locus_tag="AC16VR_14570"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014603513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HIR6012432.1"
                     /translation="MAYPRDLRDEDAAETREWLDSLEYVLENAGVDRANFLLERLSAR
                     MTRTGARLPYTITTPYRNTIPSSREAVMPGDLFMERRIRSLIRWNALAMVVRANRRPG
                     DLGGHISSFASSATLYDVGFNYFFRGPTDEHPGDLIYFQGHASPGIYARSFLEGRLSE
                     AQLDNFRREVAGEGLSSYPHPWLMPDYWQFPTVSMGLGPIQAIYQAHIMKYLDARGLL
                     PMGERKVWAFLGDGECDEPEALGALSKAGTEKLDNLIFVVNCNLQRLDGPVRGNSKII
                     QELEGNFRGAGWNVIKVVWGRLWDPLFANDTAGLMQLRMNEVVDGEYQNYKAKGGAYT
                     REHFFGAHPELLAMVERLSDDDIMRLNRGGHDPFKVYAAYHEAVHHKDEPTVILAKTV
                     KGYGMGDAGEAENDTHQVKKLNLDELKRFRDRFDVPLSDTELEDIPFYRPPKDSPEMV
                     YLRKKRADLGGPIPQRTTASEALQVPELSAFAQQLQGTGDRSNSTTMAFVRMLGTLVR
                     DKNIGKRIVPIVPDEARTFGMEGMFRQLGIYSSAGQLYEPEDYGEIAAYRESRDGQVL
                     EEGINEAGAFSAWLAAATSYSSHRFTLIPFYIYYSMFGFQRVGDLAWAAGDIQARGFL
                     LGGTSGRTTLNGEGLQHQDGHSHVLASTIPNCVTYDPCYAYELAVIVHDGLKRMVENG
                     ENVFFYITVMNESYRQPAMPEGVEEGILRGIYRVRTVGDGETRRVRLLGSGTILREVE
                     AAADMLHEDYGVSAEVFSVTSYNELARDGQDVERWNLLHPEEPPRVSYVSQQLAGDDP
                     VIASSDYMKSLAEQVRPYIGAPYKVLGTDGFGRSDTRESLRHFFEVDRKFVALAALRS
                     LADAQKIPLEEVRQARDAMGIDADKPNPRTA"
     gene            4731..>5847
                     /locus_tag="AC16VR_14575"
     CDS             4731..>5847
                     /locus_tag="AC16VR_14575"
                     /inference="COORDINATES: protein motif:HMM:NF012584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HIR6012433.1"
                     /translation="MATEIRIPDIGDVEHVEVVEICVAAGAEVAADDALIIIESDKAS
                     MEVPAGVAGVVASLAVAVGDTVAEGDLIAVIEAAGAAPVEDQAGEVAAEAPPQMQDPK
                     VPPERAVASPPTKPEAGPSGTVEIRVPDIGEAEDVVVVEVAAAAGQQVGEDDLLVVVE
                     SDKASMEIPAGCSGTVLEMHVSEGAAVTEGTLIATVEVKGSSAAPAPAADRPEVEAGE
                     VPRGAEAAAPQPISTTAAPPAGPDTAAAEQESAGTALGERIYAGPAVRRLARELGVDL
                     ARVRGTGARERIVKDDVKAYVKDAMTAAPGGAGAGAASGAGIPAIPQIDFSRFGPVEI
                     RPLTRIRAAGAANLQRSWLNLPHVTQHDEADVTELEAF"
BASE COUNT          995 a         1863 c         2040 g          949 t
ORIGIN      
        1 gagcagccga gccgccgtac ccgcgatcag atcgacgtag cgccgcgtcg ccgtggccgt
       61 ccctgacaac agctgaacga aattcagcat ggtccgttcg gccgtgagca gacccctcgc
      121 ggggccgctc agagcaaaga gcgtctgccc cggcgagacg cgatcgccgt ccgcgcagtg
      181 aagggtcacc tcgatggcgg cgtccacctg ggcgcaggtt tcgagcaccc agggcgcccc
      241 gcagaaaatg cccgcgtccc gggtgataac ctctgcggtt gcccgggcgt caggcgcgat
      301 cagctccgcg gtgatgtccc cggtgcccac gtcctcggca agagccgcgc gcacgctttc
      361 gccgacggcg tctgccagct cgtcggcgtg tgggaacccg tcgggcgcca tcgggctgcc
      421 tccgatatcc gatagttgtg cgcgggccat tctacttcaa tagaatctgc ttcagtgccg
      481 gtcacggatc acaggttttg cgcgtcagga aagaccattg cctcgtcggg gttccgttcc
      541 gggcctcgcc caactgcgac gagcgtcccg atcccgagga cgtctcgctg atcgtgatcc
      601 acgggatatc cctgccgccg ggtcgtttcg gcggcgatct cgtcgacggc ctcttcacca
      661 actccctgcc cgatgagctg gaccccgggc tggcagagct tcgtgacgtg cgcgtttcgg
      721 cccacctgct catcgatcgc gccggccgaa tcacccagta cgttcccttc caccggcgcg
      781 catggcacgc gggatcatcc ggttggtgcg gccggccctc gtgcaacgac tacgccatcg
      841 gcatcgagct cgaagggacc gatgagctgg actatgccga cgcgcagtac cgggcgctta
      901 ttgcggtgag ccgggccctg ttcgatcgct acccgcggct gtccgtggac gccgttgttg
      961 ggcaccagga aatcgcgccg gagcgcaaga cggatccggg ccccgccttc gactggtgcc
     1021 gttacctcgg cgcgctggcg ggcccgtgaa acgcatcggc atcgacctgg gcggcaccaa
     1081 gatcaagggc atcgtgctgg cggaggacgg ccgggtgctg tcgacgcgac gggcggcgac
     1141 gccgtcgcaa agctacgccg gcaccgttgc ggccatcgtg cgcatggttg ccgagctcga
     1201 cggcgactcc ggcgaggcgc gtgatctgcc cgtgggcatc ggcacccccg gcatctggat
     1261 ggcgcagcgg agcgcgatga agaactgcaa ctccacctgg ctcaacgggc aaccgctgct
     1321 gctcgatctg gagcatgcgc tcggggcccg cgtacgcatc gccaacgacg ccgactgttt
     1381 tgctctctcg gaagccatgg ccggaacggg ccggggatat gggagcgtct tcggcgtcat
     1441 cctgggcacc ggcgtgggtg gcggcttcgt gaccggggga cggctgatga gcgggccgaa
     1501 tgggctggcc ggcgagtggg ggcacacgcc gctgccttat ctgggcctgc acgaagcgct
     1561 ggaaatctcg gtgggaagcg aactggatcg ccggcacgag caggcgaatg cgctagaatc
     1621 tcggctggcg gtcagaacct gctactgcgg acggccgggc tgcgtggaaa cctacctcag
     1681 cggccccggg tggcggcaga cgcaccgcga gctttggagc gaggacgtgc ccacggagac
     1741 gattgccaag cgtcgcgacg agcgctcgga gcagacgttt ttcctgtaca cccgaatgct
     1801 cgcgcgcagc ctggctcaga tcgtgaacat cgttgacccg gcggtgatcg tgctgggcgg
     1861 tgggttgtcg aacgtgcagg cgctgtatca gccggtcaac gagatgctcg cgcgctacgt
     1921 cttttcgggc gagtgcgaca cgcaggtgaa agcgcctcag ctgggcgcgg agagcggggt
     1981 gctcggggct gcacagctgt ggagcgataa cggaggttga ggtccggccg gctagccggc
     2041 cagcgggaac tgatatggcg tatccaagag atcttcgcga cgaggacgcg gcggagaccc
     2101 gggagtggct ggattctctc gagtacgttc tcgagaacgc gggcgtggac cgtgccaact
     2161 ttctgctgga gcgtttgtct gcccggatga cgcgcaccgg cgcgcgtttg ccctacacca
     2221 tcaccacgcc ttaccggaac accattccct cgagccgcga agccgtgatg ccgggcgatc
     2281 tcttcatgga acggcgcatc cgctcgctga tccgctggaa cgcccttgcc atggtggtgc
     2341 gggcgaaccg gcgtccgggc gatctgggcg ggcacatctc cagcttcgcc tcgtcggcca
     2401 cgctttacga cgtgggcttc aactatttct tccggggccc gacggacgag cacccgggcg
     2461 acctgatcta ctttcagggg cacgcctcgc cgggcatata cgcccggtcg tttctcgagg
     2521 gccgcctgag cgaggcccag ctggacaact ttcgccggga agtggcaggg gaggggctct
     2581 catcctatcc gcacccctgg ctgatgcccg actactggca gtttcccacg gtctccatgg
     2641 ggctggggcc gatccaggcc atctaccagg cgcacatcat gaagtacctg gatgcccggg
     2701 ggcttctgcc catgggcgag cgcaaggtgt gggcctttct cggcgacggc gagtgcgacg
     2761 agcccgaagc gctcggcgcc ctgtccaagg ccggcaccga gaagctcgac aatctgatct
     2821 tcgtcgtcaa ctgcaacctg cagcggctgg acggtcctgt gcggggcaac agcaagatca
     2881 tccaggagct ggaaggtaac ttccgcggcg cgggctggaa cgtgatcaag gtggtgtggg
     2941 gtcggctctg ggatccgctg ttcgccaacg acaccgcggg cctgatgcag ctgcgcatga
     3001 acgaggtggt ggacggcgag taccagaatt acaaagccaa gggcggggcc tacacccgcg
     3061 aacacttctt cggcgcgcat ccggagctcc tggccatggt cgagcgcctc tcggacgatg
     3121 acatcatgcg gctcaaccgg ggcggccacg accccttcaa ggtgtacgcg gcctatcacg
     3181 aggccgtgca tcacaaagac gagcctacgg tgatcctggc gaagaccgtc aagggatacg
     3241 gcatgggcga cgctggcgag gcggaaaacg acacccacca ggtgaagaag ctcaacctgg
     3301 acgagctcaa gcgtttccgc gatcgcttcg acgtcccgct ttctgatacg gagctggagg
     3361 acatcccgtt ctaccgtcct ccaaaggaca gcccggagat ggtgtacctg cgcaagaaac
     3421 gggccgacct cggcggtccc attccccagc gcacgacggc atcggaagcg ctgcaggttc
     3481 cggagctcag cgccttcgcg cagcagctgc agggaaccgg agaccggtcc aactcgacca
     3541 ccatggcgtt cgtgcgcatg ttggggaccc tggttcgcga caagaacatc ggcaagcgaa
     3601 tcgttcccat cgttcccgac gaggcccgca cgttcgggat ggagggcatg ttccgccagc
     3661 tgggcatcta ctcctcggcc gggcagctct atgagcccga ggattacgga gagattgccg
     3721 cctatcgcga gtcccgggac ggccaggtgc tcgaggaggg catcaacgaa gccggcgctt
     3781 tctcggcctg gttggcggcc gccacgtcct acagctcgca ccgcttcacg ctgattccct
     3841 tctacatcta ctattccatg ttcggtttcc agcgggtggg cgatctggcc tgggccgctg
     3901 gggacattca ggcgcgcggt tttctgctcg ggggtacgtc gggacggacg acgctcaacg
     3961 gcgaggggct gcagcatcag gacggtcaca gccacgtgct cgcctcgacg atccccaact
     4021 gcgtcaccta tgacccctgc tacgcgtacg agctggccgt gatcgttcac gatggtctga
     4081 agcggatggt cgagaacggc gagaacgttt tcttctacat cacggtgatg aacgagagct
     4141 atcggcagcc ggccatgccc gaaggggtgg aggagggcat cctgcggggc atctaccggg
     4201 tgcgcacggt tggcgatggc gaaacgcggc gggtacgcct gctcggcagc ggaaccatac
     4261 tgcgcgaggt cgaagcggct gccgatatgc tgcatgagga ctacggcgtc agcgccgagg
     4321 tgttcagcgt cacgagctac aacgagctgg ctcgcgacgg ccaggacgtg gagcgttgga
     4381 acctgctgca tccggaagag ccgccgcggg tgagctacgt ctcgcagcag ctcgccggag
     4441 acgatccggt gatcgcctcc agcgattaca tgaagtccct ggccgagcag gtccggccct
     4501 acatcggggc gccgtacaag gtgctcggca cggatgggtt cggacgcagc gataccaggg
     4561 aatcgctcag acacttcttc gaggtcgacc gcaagttcgt cgcgctggcc gcgctgcgca
     4621 gcctggcgga tgcccagaag atcccgctcg aagaggttcg ccaggccagg gatgccatgg
     4681 gcatcgatgc cgacaaaccc aatccgcgga cagcctgagg gagcctggag ttggccacag
     4741 agatacgcat tcccgacatc ggcgatgtgg agcacgtgga ggtggtggag atctgcgtcg
     4801 cagcgggtgc cgaggtggcc gcggacgacg cgctcatcat catcgagtcc gacaaggcgt
     4861 ccatggaggt gccggcaggg gtggcgggcg tggtggcttc cctggcggtg gccgtgggcg
     4921 acaccgtggc cgagggcgat ctcattgccg tcatagaggc tgccggggcg gcgccggtcg
     4981 aggatcaggc cggcgaggtg gccgctgagg cgcccccgca aatgcaggat ccgaaagtgc
     5041 ccccggaacg ggccgtcgcc tcgccgccga caaagcccga ggctggcccg tccggtaccg
     5101 tcgagatccg ggtgccggac atcggcgagg ccgaggacgt cgtggtggtg gaggttgcag
     5161 ccgccgccgg gcagcaggtt ggcgaggacg atcttctggt cgtggtggag tccgacaagg
     5221 cgtccatgga gataccggca gggtgttccg gaacggttct ggaaatgcac gtatcggaag
     5281 gtgcggcagt taccgaaggg accctcatcg ccacggtcga ggttaaggga tcgtcggcgg
     5341 cccccgcacc tgcggcagat cggcccgagg tcgaagcagg cgaggttccc aggggcgccg
     5401 aagcagcggc cccgcagcct atctcaacga cggctgcgcc cccggccggc ccggacactg
     5461 cggcggcgga gcaagagtcg gccgggacgg ctctgggcga acgcatctac gccggcccag
     5521 ccgtgcgccg gctcgcccgt gagctggggg tggatctcgc ccgggtgagg ggcaccggcg
     5581 cgcgggagcg catcgtgaag gatgacgtca aggcgtacgt caaggatgcc atgaccgcgg
     5641 cgccgggcgg tgccggagca ggggccgcga gcggagcggg catcccggcc ataccgcaga
     5701 tcgacttttc cagattcggc ccggtggaga tccggccgct gacgcggatt cgcgcggcgg
     5761 gcgccgccaa cctgcagcgc agctggttga acctgcccca cgtcacccag cacgacgagg
     5821 cggacgtcac ggagctcgag gcctttc
//
LOCUS       DBNHQR010000638         2304 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_633034, whole genome shotgun sequence.
ACCESSION   DBNHQR010000638 DBNHQR010000000
VERSION     DBNHQR010000638.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_633034"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1297
                     /locus_tag="AC16VR_14580"
     CDS             167..1297
                     /locus_tag="AC16VR_14580"
                     /inference="COORDINATES: protein motif:HMM:NF015368.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003847 -
                     1-alkyl-2-acetylglycerophosphocholine esterase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6012434.1"
                     /translation="MHRDLMTSTTFRHLTHETVAAGVTGQEIEAAIDELFSRERGSCL
                     NAPLATHGGPFPVLIASPGDAGSRHNLESVCEHLASHGYAVIVPEHTGSSPFAQLGRD
                     PWLDETGPSAKQAEHVRLVREITDSRGAYGARAGFGQTYSPLRPHREQGTAERQLNDA
                     LHERVRDLGTALNWLAAANARGPWAGRLRADRAGLLGRSFGAATALRAACADERLACT
                     FAVAAPAWRNAPFLELRKPTFLLSGAEDDLLIGMASSQAGAKPTPENPHPALFEAFIR
                     SRAPAMWGLLANADHDAFTCAARYWWPGLKPGQARRALAPDETFELIEPQRAQAVQQL
                     TARAFFDCFLRMDATALARLDPATLAAAGLTFDTRNLEQVAR"
     gene            1294..1479
                     /locus_tag="AC16VR_14585"
     CDS             1294..1479
                     /locus_tag="AC16VR_14585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012435.1"
                     /translation="MTLAEITALISGGGHVEVELHAVDPMIYVAYLVSGNRRQPVTGR
                     ERVSLQAEALRSFQPRR"
     gene            1515..1991
                     /locus_tag="AC16VR_14590"
     CDS             1515..1991
                     /locus_tag="AC16VR_14590"
                     /inference="COORDINATES: protein motif:HMM:NF019473.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="limonene-1,2-epoxide hydrolase family protein"
                     /protein_id="HIR6012436.1"
                     /translation="MIDLSMAGNEDEALALALLKAMEDGDLAAKIEELCTDDFVWANS
                     GLPTLSGKTAVRAHMEAGGFSRQIPILAAMTSFSADVLHIASRAGVVFTERVDHHWDA
                     DGRDLMTPHIAGVMEIRDGRIAALRDFYDVACYAQTPMAPNPDHAMAGTNDKGELT"
     gene            1988..>2304
                     /locus_tag="AC16VR_14595"
     CDS             1988..>2304
                     /locus_tag="AC16VR_14595"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6012437.1"
                     /translation="MKRTVDAEHLIKPAKFAHFVLRVSDIEASIDWYATVLGMEMVHR
                     ADMLAFMTYDEEHHRLALVQTPVEAATPGAPGLDHVAYTLGSLGDLMATYKRLKAADI
                     LPV"
BASE COUNT          398 a          809 c          738 g          359 t
ORIGIN      
        1 cgttgcccgt tttgcctcgt caaccgtcag ataatccatc tcccctctac cccagcgatg
       61 tcgatcccaa gttatacccc gaaaacgtca ggcatctcga ccgcggcgcg acccggtctg
      121 cgcgaccagg gccacctacg ccgactacgt gttcggggat gccagcgtgc accgcgacct
      181 catgaccagc acgacgttcc ggcacctgac tcacgaaacc gtcgcggccg gcgtgaccgg
      241 ccaggagatc gaagccgcaa tcgacgaact cttttcgcgc gagcgcggca gctgcctgaa
      301 tgcccctttg gcaacacacg gcggaccctt tcccgtgttg attgcatctc ccggagacgc
      361 cggcagccgg cacaacctgg agagcgtctg cgagcatctg gccagccacg ggtacgcggt
      421 gatcgtgccc gagcacaccg gcagcagccc gtttgctcag ctcggacggg atccctggct
      481 ggacgagacc ggcccctctg caaaacaggc agagcacgtc cgcctggtga gggagatcac
      541 ggacagccgc ggagcctacg gagcaagggc cggcttcggg cagacctact caccgctgcg
      601 accgcacagg gagcaaggca cggcagagcg acagctgaac gatgcgctcc acgagcgggt
      661 gcgcgacctg ggcaccgcct tgaactggct ggctgccgcc aacgctcggg gaccctgggc
      721 aggacgcctg cgcgccgacc gcgccggtct cctcggccgc tcattcggtg ctgccacggc
      781 tctgcgcgcc gcctgcgccg atgagcgcct ggcctgcacg ttcgccgtgg cggcgcccgc
      841 ctggcgcaac gccccgtttc tcgaattgcg caagccgacg ttcctgttga gcggcgccga
      901 agacgatctg ctgatcggca tggccagcag ccaggccggg gcgaagccga ccccggagaa
      961 tcctcaccct gcgctctttg aagccttcat ccggagccgt gcgccagcca tgtggggtct
     1021 gctcgcgaac gccgaccacg acgcgttcac ctgcgcagcc cggtactggt ggccggggct
     1081 gaagccgggc caggcgcgac gcgccctcgc tccggatgag accttcgagc tgatcgagcc
     1141 gcagcgggcc caggccgttc agcagctcac cgcgcgggcc ttcttcgact gcttcctgcg
     1201 gatggacgca acggcgctcg ccaggctgga tccggcgacg ctggcggcgg ctgggctgac
     1261 cttcgacacc cgcaacctcg agcaggttgc ccgatgacgc tcgccgagat caccgccctc
     1321 atcagcggcg gcggccacgt cgaggtcgag ctccacgccg tcgacccgat gatttacgtg
     1381 gcttaccttg tttcaggcaa ccggcggcag cccgtcacgg ggcgcgagcg ggtgtcgctg
     1441 caagccgagg ccttgcgctc gttccagccc cggcgttagg gttcgggctg agcgcttctg
     1501 ggagacagca ccggatgatc gacctctcca tggcgggcaa cgaagacgag gcgctcgcgc
     1561 tggcgctgct caaggcgatg gaggatggtg accttgccgc caagatcgag gagctgtgca
     1621 ccgacgactt cgtctgggcc aacagcgggc tgccgacgct gagcggcaag accgccgtgc
     1681 gcgctcacat ggaagccggt ggcttttccc ggcagatacc catcctagct gctatgacgt
     1741 ctttcagcgc cgacgtgctg cacattgcca gccgggccgg ggtggtgttc accgagcgcg
     1801 tcgatcacca ttgggacgcc gacggccggg acctgatgac accgcacatc gcgggcgtca
     1861 tggaaatccg cgacggcagg atcgccgccc tgcgtgactt ctacgacgtt gcctgctacg
     1921 cgcagacgcc catggccccg aatcccgacc atgccatggc aggcaccaac gacaaaggag
     1981 agctgacatg aagagaaccg tcgacgcaga gcatctgatc aagccggcca agttcgcgca
     2041 cttcgtgctg cgcgtatcgg acatcgaagc atccatcgac tggtacgcca ccgtgctcgg
     2101 catggagatg gtgcaccggg ccgacatgct ggcgttcatg acctacgacg aggagcatca
     2161 ccgcctggcc ctcgtgcaaa cgcccgtcga ggctgccacc cccggtgctc cgggactcga
     2221 ccacgtagcc tacacgctgg gctccctggg cgatctcatg gctacctaca agcgcttgaa
     2281 ggcagcggac atcctgccgg tgtg
//
LOCUS       DBNHQR010000639         9037 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1390704, whole genome shotgun sequence.
ACCESSION   DBNHQR010000639 DBNHQR010000000
VERSION     DBNHQR010000639.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9037)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9037)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9037
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1390704"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="AC16VR_14600"
     CDS             complement(<1..619)
                     /locus_tag="AC16VR_14600"
                     /inference="COORDINATES: protein motif:HMM:NF012345.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase-related protein"
                     /protein_id="HIR6012438.1"
                     /translation="MRIGILQTGAVLEDIQPRFGDYPAMFVDLLSGDAHRRPSFSTYR
                     VREGELPEPAACDAYVITGSRHSVYEDHPWIVELAEFVGNTMAGGQKIVGICFGHQLI
                     AHYFGGRTEPASVGWGVGVHGVEIVSRERWMDPHQGSVSMLSSHKDQVVALPRGALRV
                     ASSAFCPNAAFAIGDVVMTWQGHPEFEKDYAETLMRRREALLGPET"
     gene            complement(637..2013)
                     /locus_tag="AC16VR_14605"
     CDS             complement(637..2013)
                     /locus_tag="AC16VR_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HIR6012439.1"
                     /translation="MNDGMELLDQKLARYRERGVSRVKLGLTDVDGVIRGKYVSLSKF
                     ASLMEKGGGFCDCVFGWDVHDQLYDAGTYTGWHTGFPDAQYRLVADSERWLSEEGCPF
                     FLGEFVAGDGGPHPLCPRTLLKTVLGRLGDRGLTLKSGFEYEFFVFAETPHSVRDKGY
                     RNLRPLTPGNFGYSLLRASGEADKFSGLMDFSQALDCDLEGLHCETGPGVWEAALQTA
                     LGEEAADRANIFKTFAKVYFNRLDCMASFMAKWSMDYPGQSGHFHLSLLDADGRNVFF
                     DDGQPSGMSDLQRQAVAGLERYLPEYLALIAPTINSYTRLVKGAWAPTASTWGIDNRT
                     VAIRVINGSESHQHIECRVPGADSNPYLVAAAVCAAALGGIEQALLPGDPVVGNSYDV
                     EESLPEELRFPRDLAAAADRLEASDHARAMLGEAFVDHYVMSRRWECEEYRKNLNSWQ
                     LERYFEII"
     gene            complement(2420..2671)
                     /locus_tag="AC16VR_14610"
     CDS             complement(2420..2671)
                     /locus_tag="AC16VR_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012440.1"
                     /translation="MRVFLIALMACLVTLSFVGHGHAADAAAAAPPLVSTGAEAAHQV
                     AAEEGRVDWHLDTAIFTDISPIDTPEVAIKLPEDQQEEG"
     gene            complement(2691..4607)
                     /locus_tag="AC16VR_14615"
     CDS             complement(2691..4607)
                     /locus_tag="AC16VR_14615"
                     /inference="COORDINATES: protein motif:HMM:NF015803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HIR6012441.1"
                     /translation="MKFSPETVRLRRVAKAYAAGEFSRSEYRATRREVIANFRADASS
                     DDDTQRRDAVTVGSGAAVDPDQPRGRSATWLVMVALTMMVLGLAALEARAGTVIGAVS
                     ERSSDPMSSPRLAVRSLRVRAFQPYPGVLESDLNALIDDALDEIRRRNAEGDHGFTRT
                     ELDEVGRFLSALGAHRDGGALSATDAEDLMALIEQQKARRGISVLELEEIAGRVQAYY
                     RDAGYFLAVAYLPAQQVEHGIVEVGVLPGVLGDVVVTGVDQRELASRFEEVVGEPVTE
                     ESITRRVYLLNQVPGLKAQAAFEPGSEVGETRLNVNVVEQRKWSARVIADNHGDDATG
                     KERLSVVGGWFNPRGVGDVLEVGLLQTVNPTNQTYGFIDYRTPIGGVRQAHVRASTNS
                     FTASDGVELDGSGALFDVGTELALRRSRASSLTARLGVSHHRFSWATAQDQAATFLSA
                     GFDGHRLWDDRLVAAGFGAVADAGRFGDGTFAGQDATFWRLGLNAYAWRALNVPGLPG
                     EQKLAARLLGQLSGSQLPSSRRLSLGGFGRVRGFERDAFLADRGALLSLELRTPVPLG
                     EMALFADTSYGEGLNDLTPAWGHLTSLGVGWDADLLPRLTSRVSLALPVAAKGSGDMD
                     DKGFRIFWQLQYEH"
     gene            complement(4613..7126)
                     /locus_tag="AC16VR_14620"
     CDS             complement(4613..7126)
                     /locus_tag="AC16VR_14620"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HIR6012442.1"
                     /translation="MSRPAHQDATVIRTDPSLSVSFAGEGVGETNAPRILKQRFVLEE
                     KLGSGGMGTVFRAKDLRKVEARDRQPYLAIKVLNDNFRAHPEAFIALQREAAKSQAVS
                     HANIVSIYDYDKDGDVPFITMELLEGQELADLLRAYPNGLPDPMAWEVIRGICAGLGH
                     AHSTGVVHADFKPGNVFVSPRNAAKVLDFGIARAVRLNQIYGEETVIDPVRLGALTPA
                     YASREMLQGDNPEPRDDIYALGVVIYLVLTGHHPYGRTPADEAAREGLAPDRPRRLTR
                     RQWRVLEKCLRFNRHERPATIDEVEHYLLKPAPWRSRTALAAAAAFALSLGINYLVGD
                     AELTEVREEVRQSTLVDAQTARVQAMLSEPDFEPGWEDRIASELDRLNVLDESGASGA
                     RMSAQIIALLTQRIVATRDLGNAFELYRSATRFGEPERAGRVLHDRLLARVFALLESP
                     QPEQRWFDSVRRELARQASTFPESKEIEPIRLEVGDAVEAQLLRSIDRGDFPAAKAAL
                     AELEPLRFDVAAVERLARAVEDAEFGYAAAEQLRAVEENAERFEAAIADVLDTSCLRL
                     DLGPVAALFDAWVAEDAGLGSPGRQMIGAKVAGCVAQLGEHDLARAAALADSARTRFG
                     ELPQLAEVRLDPCSARYLVGAGAQAGRSGYCADRLVGDLVGPRLVVVPNGEARFAITR
                     HEISRGQLNAYCAYSGACGVADADQLPATGVDVVVAQGYARWLSEITGFTYRLPTLTE
                     WRLAAQGAPDASRNCRVEVDGVERGLALVPASSGQAGEFGLINALGNAQEWVLDGGKL
                     RVAGGAFDDAIGDCVPTTVRDHQGRADAVTGFRLVREIS"
     gene            complement(7123..7845)
                     /locus_tag="AC16VR_14625"
     CDS             complement(7123..7845)
                     /locus_tag="AC16VR_14625"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR6012443.1"
                     /translation="MELSWQSAEACHCGKRRASNDDAVVSRPSARLWAVADGMGGHKA
                     GDVASGAIADALGRLTLNGNLADDVDAVDDALGEVNEQLREHARETCPGATVGSTVVA
                     MLALAGVGVALWAGDSRLYRLRGRRLEQITRDHNPVSDLLDSGAVSESEAVAADTNIV
                     TRAVGGHSELFLDVAVFDIDPQDTYLLCSDGLYRELNTADLAHGLQGESPAEIANGLL
                     SRCLETAARDNVSLIVARPDVT"
     gene            complement(7851..8183)
                     /locus_tag="AC16VR_14630"
     CDS             complement(7851..8183)
                     /locus_tag="AC16VR_14630"
                     /inference="COORDINATES: protein motif:HMM:NF012708.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR6012444.1"
                     /translation="MEAHDGSQLLLAWQDGRVTLTGHDQVLVLGAAQSADVPVRGNFA
                     SRNHARIERRKQEYVLIDHSTNGTFVQTEDERVTFVRRREVRLWGAGWISLGEPLSPA
                     TAIHFENV"
     gene            complement(8329..>9037)
                     /locus_tag="AC16VR_14635"
     CDS             complement(8329..>9037)
                     /locus_tag="AC16VR_14635"
                     /inference="COORDINATES: protein motif:HMM:NF025912.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6012445.1"
                     /translation="LVAQYQGKALLGLVDVAQDPTLAQHLRVQGVPSIRVIKGGQLVD
                     QLDGPQPEHVLRALLDQLTLSPADQLHDQLQLLLGSGDFQSALQLLQQAINEEPNNAG
                     FRVELADVLVLKGELDEARKVLAGIAKDADDIDRPQIRLEMAEEAAGMPSMSELAEAH
                     QRDPEDLETRYGLAVVGATAGNYEFALEHAMAILQQDREFRDDIGRTTMIRIFSLLGK
                     GSDLAQRYRRKMFAFMH"
BASE COUNT         1599 a         3100 c         2826 g         1512 t
ORIGIN      
        1 gggtctcggg ccccagaagc gcttcgcggc ggcgcatcaa cgtttccgca tagtccttct
       61 cgaactccgg gtggccctgc cacgtcatga ccacatcgcc gatggcaaac gccgcgttcg
      121 ggcagaaggc gctggaggcc acgcggagcg cgccgcgcgg cagggccacc acctgatcct
      181 tgtgactcga cagcatgctc accgatccct ggtgcgggtc catccaacgt tccctgctca
      241 cgatctccac cccatggacg cctacacccc aacccaccga ggccggctcg gtgcggccgc
      301 cgaagtaatg ggcaatgagc tggtggccaa agcagatccc cacgatcttt tgtccgccgg
      361 ccatggtgtt accgacgaac tccgccagct cgacgatcca gggatgatcc tcgtaaacgc
      421 tgtgtcggct gcccgtgatg acgtaggcgt cgcaggccgc cggctcagga agctcgcctt
      481 cccgcacgcg gtaggtcgag aaactcgggc gccgatgcgc atctccgctg agcagatcca
      541 cgaacatggc cgggtagtcg ccaaaccggg gctgaatatc ctccagaacg gcgccggtct
      601 gcagaatgcc gatgcgcatc aggcaatgtt tccccgtcag atgatctcga agtagcgttc
      661 cagctgccag ctgttgaggt tcttgcggta ctcttcgcac tcccagcgcc gggacatgac
      721 gtagtggtcg acgaaggcct cgccgagcat cgccctggca tggtcggaag cctccagacg
      781 atcggccgcg gccgccaggt cgcggggaaa cctaagctcc tcgggcagag attcctccac
      841 gtcgtaggaa tttccgacca cgggatctcc gggcaggagg gcctgctcga taccccccag
      901 cgccgcggcg cacacggcgg cagcgacgag ataggggtta gagtcggcgc ccgggacacg
      961 gcactcgatg tgctggtgcg attcagatcc attgatgacg cgaatggcca cggtgcggtt
     1021 atcgatgccc caggtcgaag cggtgggcgc ccaggctcct ttcacgagtc gggtgtagct
     1081 gtttatggtg ggagcgatca acgcgaggta ctccggcaga taccgctcta agccggccac
     1141 cgcctgacgc tgcagatctg acatgcccga cggctggccg tcgtcgaaga aaacgtttcg
     1201 cccgtcggca tcgagcaagc tcaaatgaaa gtgaccgctc tggcccggat agtccatgga
     1261 ccacttcgcc atgaagctcg ccatgcagtc cagccggttg aagtagactt tcgcaaaggt
     1321 cttgaagata ttggcacgat cggccgcttc ctcgccgagc gcggtctgca atgccgcttc
     1381 ccacaccccg ggacccgttt cgcagtgaag gccttccaga tcgcagtcca gcgcctggga
     1441 aaagtccatg agaccggaga acttgtcggc ttcgccgctg gccctcaaca gggagtaacc
     1501 gaagttgccc ggggtcagcg ggcgcaggtt ccgatatccc ttgtcccgca ccgaatgcgg
     1561 cgtctcggca aagacgaaaa actcgtactc gaagccagac ttgagggtga gcccacggtc
     1621 accgagcctg cccagcaccg tcttgagaag cgttcgcggg cacaggggat ggggaccacc
     1681 gtcaccggcc acgaactcgc ccaggaaaaa cgggcacccc tcctccgaga gccagcgctc
     1741 gctgtcggca accagccgat actgggcatc aggaaaaccc gtatgccacc ccgtataggt
     1801 accggcgtcg tagagctggt cgtgcacgtc ccatccgaag acgcagtcac agaagccccc
     1861 acccttctcc atcagcgagg cgaacttgga caaactgacg tacttcccgc ggatcacgcc
     1921 atcgacgtcg gtcagcccca gcttgacacg gctgacaccc cgttcacgat accgcgcgag
     1981 cttctgatcc agcagttcca taccgtcgtt caagtaagat ctcctcgttt ggcctcaaca
     2041 tgtggagcag actttacctc aggcgaagat ttccacgaat tctgtggata actctgagga
     2101 taagtcgggc acaagttggc agagccggcg ttccacggca accaaagaat attggtcact
     2161 ttttgttcaa attcaaaaaa gatcatcagg atcagatggt tagatcccca aactaaccga
     2221 ccatatgaaa aatgctcgcc atctccccat cccaagttgc cattcttcgc cagtgtgcat
     2281 aacacggccg tcgcgcttgt tagtgttcgc taacaagccg tgctccgcgg cacttaatcc
     2341 ggcagcggaa acgcccgccc cagatgccgc tcagtacaac cgttagaccg cagtggccgc
     2401 cgccggcacg atccccgggt cagccctctt cctgctggtc ctcgggcagc ttgatcgcca
     2461 cttctggggt gtcaatcggc gagatgtcgg tgaagatcgc ggtgtccagg tgccagtcca
     2521 cgcgaccctc ctcggcggcc acctggtgtg ctgcttcggc accggtgctc accagcggtg
     2581 gcgcggcagc agcggcatcc gccgcgtggc cgtgaccgac gaagctcaag gtcaccagac
     2641 acgccatcag ggcaatgagg aatacacgca tatccaagtc ttctttaggg tcaatgttca
     2701 tattgcagtt gccagaaaat tcgaaatcct ttgtcgtcca tatcaccgga gccctttgcg
     2761 gccaccggca acgccaggct gacgcggctc gtcagccgag gcagcagatc cgcatcccaa
     2821 cccacgccca ggctggtcag gtgaccccag gcgggcgtga gatcgttcaa cccctcgccg
     2881 tacgacgtat cggcaaagag cgccatttcg cccagaggga ccggcgtgcg cagctccagg
     2941 gaaagcaggg caccgcgatc ggcgaggaac gcgtccctct cgaagccccg cacgcggcca
     3001 aagccaccga gcgacaagcg cctggagctc ggcagctggg atccgctgag ctgtccgagc
     3061 agcctggccg ccagcttctg ctccccggga agaccgggaa cgttgagcgc ccgccacgca
     3121 tacgcgttca atcccagccg ccagaaggtc gcgtcctgac cggcgaaggt cccgtccccg
     3181 aaccgaccgg cgtccgcgac ggcgccgaag ccggcagcaa cgagtcggtc gtcccacaac
     3241 cgatggccgt caaagccggc gctgaggaag gtggccgcct gatcctgcgc ggtggcccag
     3301 ctgaagcggt ggtggctgac tcccagccgt gccgtgaggc tcgacgcacg gctgcggcgc
     3361 agggccagct ccgtgccgac gtcgaagagc gccccgctgc cgtccagctc gactccgtcg
     3421 cttgcggtaa agctgttcgt ggaggcgcgc acgtgggcct gacggacacc gccgatgggc
     3481 gtccggtagt cgatgaaccc gtaagtctgg ttcgtcgggt tgacggtctg cagcagaccc
     3541 acttccagaa cgtcgccgac gccccgcggg ttgaaccagc cgcccaccac gctcagcctc
     3601 tccttgccgg tggcgtcgtc gccgtgattg tcggcaatca cgcgagccga ccatttgcgt
     3661 tgctccacca cgttgacgtt caaacgggtc tcaccgactt cgctgccggg ctcgaacgcg
     3721 gcctgggcct taagccccgg aacctgattg agcaggtaga cgcgccgcgt gatggattct
     3781 tccgtgaccg gctcccccac gacttcctcg aatcgtgagg cgagctccct ctggtcaacc
     3841 ccggtcacca ccacgtcgcc aagaacgcca ggcagcacgc cgacctccac gatcccgtgc
     3901 tccacctgct gcgcaggcag gtaggcaacg gcgagaaagt acccggcgtc ccggtagtag
     3961 gcctgcaccc ggccggcaat ctcctcgagc tccagcaccg agatgccacg gcgggccttc
     4021 tgctgctcga tgagggccat cagatcttcg gcgtccgtgg ccgaaagagc cccgccatcg
     4081 cggtgggcgc cgagcgcgct gagaaatcgc cccacctcgt ccagctcggt tcgggtgaaa
     4141 ccgtggtcac cctccgcgtt gcgccgacga atctcgtcga gcgcgtcgtc gatcagcgcg
     4201 ttcagatcgc tttccagcac gccgggatac ggctgaaagg cacgaacccg cagggaacgg
     4261 actgccagcc tcgggctgct catgggatca gagctgcgct cgctcacggc cccgatgacc
     4321 gtgccggccc gggcctcgag ggcagccaag ccgagaacca tcatcgtcaa cgcgaccatc
     4381 accagccagg tcgccgaacg cccgcgcggt tgatccggat ccacagccgc cccggagccg
     4441 acggtcacgg cgtcacggcg ctgggtgtcg tcgtccgaac tcgcatctgc ccggaagttg
     4501 gcgatcacct cgcgccgggt cgcccgatac tccgaacggc tgaactcgcc ggcggcgtat
     4561 gcctttgcga cccgccgcag gcgcacggtt tccgggctga acttcatcag cgtcacgaga
     4621 tttccctcac cagccggaat ccggtgaccg catctgcgcg gccctggtga tcccgcacgg
     4681 tcgtcggcac gcagtcgccg atggcatcgt cgaaagcacc gcctgcaacc cgcagcttgc
     4741 caccatccag cacccattcc tgagcgttgc cgagcgcatt gatcagcccg aactcgccgg
     4801 cctgcccgct gctcgccggg accagggcca gaccacgctc gacgccgtcc acctccacgc
     4861 ggcagttgcg gctggcgtcc ggcgctccct gggccgccag gcgccactcc gtcagggtcg
     4921 gcagccgata ggtgaagccg gtgatctccg acagccagcg tgcatatccc tgggcaacca
     4981 cgacatcgac gccggtagcc ggaagctggt ccgcatccgc gaccccgcac gcgccggaat
     5041 aggcacagta ggcgttcagc tgacctcggc tgatctcgtg cctggtaatg gcaaatcggg
     5101 cctcgccgtt cggaacgacc accaggcgcg gcccgacaag atcgccgacg aggcgatcgg
     5161 cgcaatagcc gctgcgtccc gcctgcgctc cagctccaac caggtagcgg gcggagcagg
     5221 gatcgagccg gacctcggcg agctggggca gctcgccgaa tcgtgtgcgg gccgagtcgg
     5281 caagggctgc cgctctcgcc aggtcgtgct cgccgagctg ggcgacgcaa ccggcgactt
     5341 ttgcgccgat catctgccgg cccggggatc ccagccctgc gtcttcggcc acccaggcgt
     5401 cgaaaagcgc agccaccggc ccgaggtcca ggcgcaggca gctggtgtcg agcacgtcgg
     5461 caatggcggc ttcgaagcgc tcggcgttct cctctacggc ccgaagctgc tcggcagccg
     5521 cgtaaccgaa ctcggcatcc tccacagcgc gagccagccg ctccacggcg gcaacgtcga
     5581 agcgcagcgg ctcgagctcg gcgagggccg ccttggcggc cgggaaatcg cctcgatcga
     5641 tgctgcgaag cagctgcgcc tccaccgcgt cgccgacttc gagcctgatc ggctcgatct
     5701 ccttgctctc aggaaaggtt gaggcctgcc gcgcaagctc ccttcgcacg gagtcgaacc
     5761 agcgctgctc aggctgcggc gactcgagca gcgcgaagac tctggcgagc aggcggtcgt
     5821 gaaggaccct gcctgcccgc tcgggctcgc cgaatcgcgt tgcgctgcgg tagagctcga
     5881 aggcattgcc caggtcccga gtcgcgacga tccgttgcgt gagcagcgcg atgatctgag
     5941 cactcatccg ggcgccactt gcgccggatt cgtccaggac gttcagccga tccagctcgc
     6001 tggctatccg gtcttcccag cccggctcga aatccggctc gctgagcatc gcctggaccc
     6061 gagccgtctg cgcatccacc agcgtcgact gccgaacctc ctccctgacc tcggtgagct
     6121 ccgcatcgcc caccaggtag ttgataccca gcgagagggc gaatgccgct gcggcggcga
     6181 gcgccgtgcg agaacgccac ggcgccggtt tgagcagata gtgctcgacc tcgtcgatgg
     6241 tcgccggtcg ctcatggcga ttgaagcgca ggcacttttc cagcacacgc cactgccgcc
     6301 gggtgagccg tctgggcctg tccggagcga gaccctcgcg ggcggcttcg tcggccggcg
     6361 tgcgcccgta gggatggtgc ccggtcagga cgaggtatat caccaccccg agcgcgtaga
     6421 tgtcgtcccg gggctccgga ttgtccccct gcagcatctc gcggcttgcg tacgccggcg
     6481 tcagcgcacc cagacgaacc ggatcgatga ccgtctcttc accgtagatc tgattgagcc
     6541 gaacggcccg ggcgatgccg aagtcgagaa cctttgcggc attgcgcggc gagacgaaca
     6601 cgtttccggg tttgaagtcc gcgtgtacca cgcccgtgct gtgagcgtgg ccgagcccgg
     6661 cgcagatgcc gcggatgacc tcccaggcca tgggatcggg aagcccgttc gggtacgcgc
     6721 gcaggaggtc ggcgagttcc tgcccctcca gcagctccat ggtgatgaag gggacgtcgc
     6781 cgtccttgtc gtaatcgtag atggacacga tgttggcgtg agacaccgcc tgggacttgg
     6841 ccgcttcccg ctgaagggcg atgaacgcct ccggatgggc gcggaagttg tcgttgagca
     6901 cctttatggc caggtagggc tgccggtcgc gagcttccac tttgcgcagg tcctttgccc
     6961 ggaacaccgt gcccatgcct ccgctgccga gcttctcctc gagcacgaat cgctgcttga
     7021 gaatccgggg ggcgttggtc tcaccgactc cctcgcccgc gaagctcacg gaaaggctcg
     7081 gatccgtccg tatgacggtg gcgtcctgat gggcgggtct gctcacgtga cgtcgggcct
     7141 cgcgacgatc agcgacacgt tgtcccgagc ggccgtctcg aggcagcggc tgagcaggcc
     7201 gtttgcgatc tcagccggcg actcgccctg aagcccatga gccaggtccg ccgtgttcag
     7261 ctcgcggtac aggccatcac tgcagagcag gtaggtatcc tgggggtcga tgtcaaagac
     7321 cgccacgtcc aggaacagct cggagtgtcc gcccaccgcc cgggtaacga tgttcgtatc
     7381 tgcggcaacg gcttccgact cggagacggc accgctgtcc agcaggtcgg agaccgggtt
     7441 gtgatcccgg gtgatctgct ccaggcggcg accccgcagc cggtagaggc ggctgtcgcc
     7501 cgcccatagc gccacgccca cgcctgccag cgcgagcatc gcaaccacgg tgctgcccac
     7561 cgtggcgccg ggacaggttt cccgcgcatg ctcgcgaagc tgctcgttga cctcgccgag
     7621 ggcatcatcg accgcatcca cgtcgtcggc caggttgccg ttgagcgtca gcctgccaag
     7681 cgcatcggct atggcgccgc tggcgacgtc gcccgccttg tgaccgccca tcccatccgc
     7741 cacggcccag agccgcgccg acggccggct caccaccgcg tcgtcgttgc tcgcccgccg
     7801 cttgccgcag tgacacgctt cggcgctttg ccagctgagt tccatgaccc ctaaacgttc
     7861 tcgaagtgaa tggccgtcgc gggactgaga ggctcgccga ggctgatcca gccggccccc
     7921 cacagacgca cttcccggcg cctgacaaag gtgacccgct cgtcctcggt ctgcacgaac
     7981 gtgccgttcg tggagtgatc gatgaggacg tactcctgct tgcggcgttc gatccgggca
     8041 tggttgcgcg aggcaaaatt gccgcgcacg ggaacgtcgg cgctctgggc ggcaccaagg
     8101 accagtacct ggtcatggcc tgtcagggtg acgcggccgt cctgccacgc caagagcagc
     8161 tgagaaccgt catgcgcttc catgtcgcag tcaccgggcc tacatatccc gtcaaggata
     8221 ggtgagcggc ctttgcaggg atgaggactg gatcacaaag cgcccgccga cggcgctatt
     8281 cacgagcgcc ggcgcggtga caggggcaaa gagacgggca acgcccggtc agtgcatgaa
     8341 ggcaaacatc ttgcgtcgat accgctgagc gagatccgaa cctttgccca gcagcgagaa
     8401 gatcctgatc atggtcgtcc ggccgatgtc gtcccgaaac tctcgatcct gctgcaggat
     8461 ggccatggcg tgctcgaggg cgaactcgta gttgccggcg gtggcgccga ccacggctag
     8521 cccgtagcgg gtttccagat cctcaggatc gcgctggtgc gcctcggcca gctcgctcat
     8581 ggacggcatg ccggcagcct cttcggccat ctccaggcga atctgcgggc ggtcgatgtc
     8641 gtcagcatcc ttggcaattc ccgcgagcac ttttctcgcc tcgtcgagct cgcctttcag
     8701 cacgagaacg tcggcaagct cgacgcggaa tccggcgttg ttcggttcct cgttgatggc
     8761 ctgctgcagc agctggaggg cagactggaa gtcgccgctg cctaagagca gctgcagctg
     8821 gtcgtgcagc tggtcggccg gggatagcgt gagctgatcc agcagggccc gcaggacgtg
     8881 ctcgggctgc ggaccgtcga gctgatcgac gagctgcccg cccttgatca cgcggatact
     8941 cggcacgccc tgtacccgca ggtgctgggc cagggtggga tcctgggcca cgtccaccag
     9001 accgagcagc gccttgccct gatactgcgc gaccaac
//
LOCUS       DBNHQR010000640         1963 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_442663, whole genome shotgun sequence.
ACCESSION   DBNHQR010000640 DBNHQR010000000
VERSION     DBNHQR010000640.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_442663"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="AC16VR_14640"
     CDS             <1..234
                     /locus_tag="AC16VR_14640"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014871007.1"
                     /note="catalyzes a two-step reaction, first charging a
                     tryptophan molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIR6012446.1"
                     /translation="AWVTEGCTTAGIGCIDCKKPLIDAINAEQEAMRERAQQFEDDRD
                     LVHSILVEGSEKARDVARETLDDVRAAIGLAHR"
     gene            231..1067
                     /locus_tag="AC16VR_14645"
     CDS             231..1067
                     /locus_tag="AC16VR_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="segregation and condensation protein A"
                     /protein_id="HIR6012447.1"
                     /translation="MSIDTEGAAAPHLLKLREQGNDTLALVRGEAVTELPKDLYIPPQ
                     ALEVFLETFEGPLDLLLYLIRRENLDILDIRVAEITAQYMSYIELMTAMQLELAGEYL
                     VMAAMLAEIKSRMLLPRPAYADEDEDDPRAELVRRLQEYEQIKNAAEGIDALPRLERE
                     IFIASAERPEVVKRHAEPDVDLKEVLVSLAEVLRRAEMFQHHEVYLEPLSVRERMTAI
                     LDRVNSASDFVSFGELFRAEEGRRGVVVTFLALMELVRESLLDFVQNEPYAPIYVRAA
                     GE"
     gene            1072..1782
                     /gene="scpB"
                     /locus_tag="AC16VR_14650"
     CDS             1072..1782
                     /gene="scpB"
                     /locus_tag="AC16VR_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HIR6012448.1"
                     /translation="MNEAVDDARLKAVIDAVLLAAGEPVSVNQILALFGPGELEGEDV
                     RARVRACLRELESEAAGRGYQLKRVASGYRYQVRQELSEWVSRLWDEKPPRYSRALME
                     TLALVAYRQPLTRGDIEQVRGVSVSPNIMRTLLERGWVRVVGQREAPGRPSMYGTTRA
                     FLDYFNLKSLDQLPPLEEIRALIEPIVVNDEAEEELRPEITVEMPADMTELAPEREDA
                     ESPEPMTAEVVPLSSLPQ"
     gene            1825..>1963
                     /locus_tag="AC16VR_14655"
     CDS             1825..>1963
                     /locus_tag="AC16VR_14655"
                     /inference="COORDINATES: protein motif:HMM:NF013633.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HIR6012449.1"
                     /translation="MSPEKSEKLQKHLADLAYGSRREMEAWIRAGRVEVNGEAAHLGQ
                     RI"
BASE COUNT          388 a          589 c          666 g          320 t
ORIGIN      
        1 gcctgggtga ccgaaggctg caccaccgcg gggattggct gcatcgactg caagaagccg
       61 ctgatcgatg ccatcaacgc ggagcaggaa gccatgcgcg aacgggcgca gcaattcgag
      121 gacgaccgcg atctggtgca ctccatcctc gtggaaggct ccgaaaaggc ccgcgacgtc
      181 gcgcgggaaa ccctcgacga cgtgcgcgct gccataggac tggcccaccg atgagcatcg
      241 acacggaagg cgctgccgct ccccatctgc tgaagcttcg tgagcagggc aacgacaccc
      301 tggcgctcgt tcggggcgaa gcggtaaccg agctgcccaa ggatctctac attcctccgc
      361 aggcgctcga ggttttcctc gagacgttcg agggcccgct cgatctgctt ctgtacctga
      421 tccgcaggga gaatctcgac attctggaca tccgggtcgc cgagatcacc gcgcagtaca
      481 tgagctacat cgagctgatg acggccatgc agctggagct ggccggagag tacctggtga
      541 tggccgccat gctggcggag atcaaatcga ggatgctgct tccacgtccg gcttatgccg
      601 acgaagatga agacgacccc cgtgcggagc tggtccgccg gctgcaggag tacgagcaga
      661 tcaagaacgc ggcagagggc atagatgccc tgccgcgact ggaaagagag atattcatcg
      721 ccagtgccga gcgcccggaa gtcgtgaagc gccacgcgga gccggacgtc gacctcaagg
      781 aggtgctcgt ttcgctggcg gaggtgctgc ggcgggccga gatgtttcag catcacgagg
      841 tgtacctgga gccgctatcg gtacgcgagc gcatgacggc catcctcgac cgggttaatt
      901 cggcaagcga ctttgtctcg ttcggcgagc tgttcagagc cgaagagggg cgcaggggtg
      961 tggtcgtcac cttcctggcg ctgatggagc tggtgcgcga atccctgctg gatttcgtcc
     1021 agaacgaacc gtacgcgccc atttacgtgc gcgcggctgg agaatgagcc catgaacgaa
     1081 gctgtggacg atgcgagact caaggccgtc atcgatgcgg tcctgctcgc ggcgggagag
     1141 ccggtatccg taaaccagat cctcgcgctg tttggccccg gcgagctcga aggtgaagac
     1201 gtcagagccc gggtgcgcgc ctgtctcagg gagctggagt ccgaagccgc cggcaggggc
     1261 taccagctca agcgcgtggc cagcggctac cgctatcagg tccgtcagga gctcagcgag
     1321 tgggtgagtc gtctgtggga cgagaagccg ccgcgctatt cccgggcgct gatggagact
     1381 ctcgctctgg tggcgtacag gcagcccctg acccggggcg acatcgagca ggtccgcggc
     1441 gtcagcgtga gccccaacat catgcgaacg ctgctcgagc gtgggtgggt tcgcgttgtc
     1501 ggtcagcggg aagcgcccgg gaggccctcc atgtacggga ccacgagagc gtttctcgac
     1561 tatttcaacc tcaaaagcct cgaccagctg ccgccattgg aggagatcag ggccctaatc
     1621 gagcccatcg tcgtcaacga cgaagccgaa gaggagctgc gcccagagat caccgtagag
     1681 atgccggccg acatgacgga gctcgcacca gagcgggaag acgccgagtc gccagagccc
     1741 atgaccgcag aggtggtgcc gctttcgagc ttgccgcaat aggggatcct tccagccctt
     1801 aggggcgttg gcccccggaa aacgatgtcc ccggaaaaat cagagaaact gcagaaacac
     1861 cttgccgacc tggcttacgg ttcgcggcgt gagatggaag cgtggatccg ggccgggcgg
     1921 gtcgaggtga acggcgaggc ggcccatctc ggccagcgga tct
//
LOCUS       DBNHQR010000641         2625 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1390996, whole genome shotgun sequence.
ACCESSION   DBNHQR010000641 DBNHQR010000000
VERSION     DBNHQR010000641.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1390996"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="AC16VR_14660"
     CDS             <1..189
                     /locus_tag="AC16VR_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003443957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HIR6012450.1"
                     /translation="ISHDRWFLDRVATHILAFEGDSHVEWFAGNFSEYETDHRRRLAE
                     QGLDTEPRRVRYKPLSRG"
     gene            complement(190..1560)
                     /gene="radA"
                     /locus_tag="AC16VR_14665"
     CDS             complement(190..1560)
                     /gene="radA"
                     /locus_tag="AC16VR_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007155110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HIR6012451.1"
                     /translation="MAGKRKAKSAYVCSDCGAEHTQWQGQCVACSAWNTLTRVSIGPI
                     KEPVSRGYAGSQSQVSLLGEVEREEAERLPTGFAELDRVLGGGLVPGSVVLMGGDPGA
                     GKSTLLLQVASRLAGRSGVLYVTGEESLHQLALRAERLQLAADQIRVAAETRAESVAE
                     LIAEYGPRVVIVDSIQVMQLESVDSTPGSVSQVRETAAFLTRLAKQTQTAVILVGHVT
                     KEGNLAGPKVLEHMIDCFMMLDSPAGSRYRTLRGLKNRFGAVNELGVFAMTEQGMKEV
                     ANPSAIFLQRGELDSPGSVATVTWEGTRPLLVEIQALVDEVQTGYAKRVAVGLDQQRL
                     AMHLAVLHRHCGITLGDQDVFANVVGGVRIGETGADLAALLAVLSSYRNRVLPKNLIV
                     FGELGLTGEVRAVANGQQRLSEARKHGFSQAIVPFANRPKEAISGMAVHGVKSLRAAM
                     DVIDTI"
     gene            complement(1560..>2625)
                     /gene="dnaB"
                     /locus_tag="AC16VR_14670"
     CDS             complement(1560..>2625)
                     /gene="dnaB"
                     /locus_tag="AC16VR_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIR6012452.1"
                     /translation="VVAYAQIVREHSTLRQLISAANRIAETAFAPDGRPSGELLNMAE
                     QEVFRIAEGRLRDSGPEKVVPLLHRAVERIEKLYETRNPITGVATGFDDLDKKTAGLQ
                     PSDLVIIAARPSMGKTALAVNIVEHAVMEGSGAVLMFSLEMPSEQIVMRMLSSLGRID
                     QTRMRTGDMHEDDWPRFTSAVSQLKDKPLYLDDTPALTPNDIRTRARRVARESGGLGL
                     VVVDYLQLMRGSGPVENRTNEISEISRSLKAIAKELRCPVVALSQLNRGLESRTDKRP
                     VMADLRESGAIEQDADVILFIYRDEVYNPESEQKGTAEIIIGKQRNGPIGMCRLSYIG
                     NLTKFENLAPDRYNDFQPFD"
BASE COUNT          429 a          881 c          852 g          463 t
ORIGIN      
        1 atatcccacg accgctggtt cctggaccgt gtggccaccc acatcctggc tttcgaagga
       61 gactcgcacg tggagtggtt tgcggggaac ttctcggagt acgaaaccga ccaccgcagg
      121 cggctcgccg agcaggggct ggatacggag ccccgccgcg tgcgctacaa gccgctttcc
      181 cgcggctgat cagatcgtat cgatcacgtc catggcggca cgcaggctct tgaccccgtg
      241 gacggccatg ccggaaatgg cctccttggg ccggttcgca aacggcacga tcgcctggct
      301 gaagccgtgc ttgcgggctt cggacagacg ctgctgcccg ttggccaccg cacgcacctc
      361 gccggtcagg ccgagctctc cgaacacgat gaggttcttg ggcagcacac gattgcggta
      421 ggacgacagc accgccagca acgccgccag atccgcgccc gtctccccga tgcgcacgcc
      481 gccgaccacg ttggcaaaga catcctgatc gccaagagtg atgccgcagt gccggtgcag
      541 cacggcgagg tgcatggcca gccgctgctg gtccaggccc acggcgaccc gcttggcgta
      601 gccggtctgc acctcgtcca cgagagcctg gatctccacg agcagtgggc gcgtgccttc
      661 ccaggtgacc gtggcgacgc tgccgggcga gtcgagctcg ccgcgctgca ggaagatggc
      721 tgacgggttg gcgacctcct tcatgccctg ctcggtcatg gcgaagacgc ccagttcgtt
      781 gaccgctcca aaccggttct tcaaaccgcg cagcgtgcgg taacggctcc cggcggggct
      841 gtcgagcatc atgaagcaat caatcatgtg ctcgaggacc ttgggccccg ccagattacc
      901 ctccttggtc acgtggccaa cgagaatgac cgccgtctgg gtctgcttgg cgaggcgagt
      961 gaggaaggcg gcggtctcgc ggacctgaga tacgctgccc ggcgtgctgt ccacggactc
     1021 cagctgcatg acctgaatgg agtccacgat gacgacccgc ggaccgtact cggcgatgag
     1081 ctcggccacc gattccgcac gggtctccgc cgccacgcga atctgatccg ccgccagctg
     1141 cagacgctcg gcgcgcagcg ccagctggtg gagcgactct tcgccggtca cgtagagaac
     1201 cccgctgcgc cctgccagcc ggctggcaac ctgcagcagc agcgtgctct ttcccgcgcc
     1261 gggatctcca cccatcagca cgacggagcc gggcaccagg ccgccgccga gcacccggtc
     1321 gagctcggca aatccggtgg gcaaacgctc ggcctcctcg cgctccacct cgccgagcag
     1381 gctaacctgg ctctggctgc cggcgtagcc ccgggaaacg ggttctttga tgggtccgat
     1441 ggaaacccgg gtgagggtgt tccaggcgct gcaggccacg cactggccct gccactgggt
     1501 atgctcggcg ccgcagtccg agcagacgta cgcggatttg gctttgcgct tgcccgccat
     1561 cagtcaaatg gctgaaagtc gttgtagcga tccggcgcca ggttttcgaa cttggtcagg
     1621 ttgccgatat aggacagacg gcacatcccg atggggccgt tgcgctgctt gccgatgatg
     1681 atctcggcgg tgcccttctg ctcggattcg gggttgtaaa cctcgtcgcg gtagatgaaa
     1741 aggatcacgt ccgcgtcctg ctcgatggcg cccgactctc taagatcggc catgaccggc
     1801 cgcttgtcgg tacggctctc cagcccccga ttgagctggg aaagcgcgac caccgggcag
     1861 cgcagctcct tggcgatggc tttcagagat cgggagatct ccgaaatctc gttcgtgcgg
     1921 ttctccacag gtccggaacc gcgcatgagc tgcaggtagt cgaccaccac caggccgagg
     1981 ccgccggatt cgcgggctac ccgccgcgcc cgggtgcgga tgtcgttcgg cgtcagcgca
     2041 ggcgtatcgt ccaggtacag aggtttgtct ttcagctggc tgacggcgga ggtgaaccgc
     2101 ggccagtcgt cttcgtgcat gtccccggtg cgcatgcggg tctgatcaat cctgcccagc
     2161 gatgagagca tgcgcatgac gatctgctcg gacggcatct ccaggctgaa catgagcacg
     2221 gcgccgctgc cttccatcac cgcgtgctcg acgatgttga ccgcaagggc cgttttgccc
     2281 atggacggcc gcgcggcgat gatcaccaga tcggacggct gcaggccggc cgtcttcttg
     2341 tccagatcgt cgaagccggt ggccacgccg gtaatggggt tgcgggtctc gtagagcttc
     2401 tcgatgcgct ccaccgcccg gtgcagcagc gggacgacct tttcgggtcc actgtcgcgc
     2461 agacgccctt cggcgatgcg gaagacctcc tgctcggcca tgttcagcag ctccccgctg
     2521 ggcctgccgt cgggagcgaa cgccgtttcc gcaatgcggt tggccgcgct gatcagctga
     2581 cgcaacgtgg agtgctcgcg cacgatctgc gcgtaggcca ccacg
//
LOCUS       DBNHQR010000642         4024 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_570031, whole genome shotgun sequence.
ACCESSION   DBNHQR010000642 DBNHQR010000000
VERSION     DBNHQR010000642.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_570031"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="AC16VR_14675"
     CDS             <1..276
                     /locus_tag="AC16VR_14675"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6012453.1"
                     /translation="RQASWLTLEAAHKADKGEPFRKEAAMAKLVATEAGSRVVDRCMQ
                     MFGGYGVTKDFPFERWYREMRIRRIGEGPNEVQRHIIARDILGASLR"
     gene            348..1391
                     /locus_tag="AC16VR_14680"
     CDS             348..1391
                     /locus_tag="AC16VR_14680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012454.1"
                     /translation="MLQGRYSEAELYSEHDYARPHQIAGRRLHGGFDAAGNYVPPRSK
                     GRSEAIEAWTRALRERGGELFDADASLLSGPRMPTVEQQHMLLREGIGRPFWNTLTVT
                     GKIEGRGRVLAEMQFPDLQDVVVEDISDKAVGHLNRGLLAIHDIDEGGQPELGLGGHD
                     VMWFVARDLVFGPGAYPDVEPPESIARPEAGKRWMPEISAEHEALLSFLMNLLIIEFR
                     AEIGFAATQDILRADDLFTDRRTRACEAAEIIERIRADEEIHVCSLRLYLGELRQLTL
                     RTVDGGTVPAAELIDGFWDGLVRWATVEQPQLAAERQYAAIRTEILEHPDGERVLAAF
                     DALSDPAGLASAG"
     gene            complement(1407..2195)
                     /locus_tag="AC16VR_14685"
     CDS             complement(1407..2195)
                     /locus_tag="AC16VR_14685"
                     /inference="COORDINATES: protein motif:HMM:NF014635.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HIR6012455.1"
                     /translation="MSDLITTDQTSYDWAELVAGLNRLLRLRTTPIGMKLFETRGEME
                     AVPRIRRPRDIHTTDQIVGQAARNGWTVGITAEDLVGAQCSTVIGLHPRSDDWLSGDR
                     MTGVWYETGEDASAHQHAMDVVPHGRYEAMAVSPLASGRLDPPDICLIYATPAQMILL
                     INGLQWSGYRKLEWGCVGESACADSWGRALATGEPSLSIPCFAERRYGGVMEDELLMA
                     MPPMFLPKTLAGLEQLSRNGLRYPIPQYGVNNDVRAGMGVSYGR"
     gene            complement(2227..3060)
                     /locus_tag="AC16VR_14690"
     CDS             complement(2227..3060)
                     /locus_tag="AC16VR_14690"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6012456.1"
                     /translation="MYEEITYTVEDPVAIITMNRPDRLNAFTVRMLAEIRHALGAAEK
                     DGRVVGIVLTGAGRGFCAGMDMGSLDRMSSGGEAAAEDLSGLEAEVGDPGMGEDFQVT
                     YGYLMAVQKPVIAAVGGACAGLGFVIAMLTDMRFVEPQAKFTTAFSQRGLVAEHGVSW
                     ILPRLIGPGRALDILWSSRKFDGTEAERIGIAERLVDEGAAVSEACDYIRDLAANASP
                     TSLKMMKAQVYRHLNMSLGEAMRESNAWMAQSLKREDFTEGVRSFIERRPPRFKRVGE
                     D"
     gene            3203..3940
                     /locus_tag="AC16VR_14695"
     CDS             3203..3940
                     /locus_tag="AC16VR_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012457.1"
                     /translation="MSRSLSWFLAFLVLLGVLAVAGSWYLGPSLVGLAFDADRREAPY
                     YVLSLAGRSELADADLRFGSDLAELFDAAGGKLLWRGTTERVVKGRLQDEWLNAQLFA
                     FPRGANFVELKTGGDYRDLLAAYEGRSRLLLGSPTQPDRMARGRIWVLNLIAEDAEDL
                     QATGGDPLAGLMGGVADFGGSLVWDAKVADLDERSSWNRLVLLGFAQPLEAEVWLSDA
                     GTQTELALSMERARRRVTLLMRPADPI"
BASE COUNT          620 a         1304 c         1396 g          704 t
ORIGIN      
        1 cggcaggcct cctggctcac cctggaggcc gcgcataagg ccgacaaggg cgagccgttc
       61 cgtaaggagg cggccatggc caagctggtg gccaccgagg cgggctcccg ggtggtggac
      121 cggtgcatgc agatgttcgg cggctacggg gtgaccaagg acttcccctt cgagcgctgg
      181 tatcgggaaa tgcgcatccg gcgaatcggc gagggcccca acgaggtgca gcgacacatc
      241 attgcccggg acattctggg ggcgtctctg cgctgaaacg ctttgccggg acggcttccg
      301 gcgcaatctg ttataaccgg cgttcctggg acgctacgag gctgcaaatg ctacagggac
      361 gctacagtga ggccgagctc tacagcgagc acgactacgc gcggccacac cagatcgccg
      421 ggcgccgcct gcacgggggc tttgacgccg ccggcaacta cgtgccgccc cgctccaagg
      481 ggcgctcgga ggccatcgag gcgtggaccc gggcgctgcg cgaacgcggc ggagagctgt
      541 tcgatgccga tgcgagcctg ctttcgggcc cgcgaatgcc caccgtcgag cagcagcaca
      601 tgctgctccg cgaaggcatc ggccggccgt tctggaacac cctgaccgtg accggcaaga
      661 tcgaggggcg cggccgggtg ctcgccgaga tgcagttccc cgatcttcag gacgtcgtgg
      721 tggaagacat ctccgacaaa gccgtcggtc acctcaaccg cgggctgctc gccattcatg
      781 acatagacga aggcggacag ccggagcttg gcctgggcgg gcacgacgtg atgtggttcg
      841 tggcccgcga cctggttttc ggccccgggg catacccgga cgtggagccg ccggaaagca
      901 tcgcccggcc cgaggccggg aaacggtgga tgcctgagat atccgctgag catgaggcgc
      961 tgctcagctt tctgatgaac ctgctcatca tcgagtttcg ggcggaaatc gggtttgccg
     1021 caactcagga catcctgcgg gcagacgacc tgttcactga ccggcgcacg cgggcttgcg
     1081 aggcggcgga gatcatcgag cgcattcgcg cggacgagga aattcacgtg tgctcgctgc
     1141 ggctttatct cggcgagctt cgccagctca cgctgcgcac ggtagatggt ggcacggtgc
     1201 cggccgccga gctgatcgac ggcttctggg acggcctggt tcgatgggcc accgtggagc
     1261 agccgcagct ggcggcggag cggcagtacg ctgctatccg gaccgagatc ctggagcatc
     1321 cggacggcga gcgggtcctg gcggcgttcg acgccctcag cgatccggcg ggcctggcgt
     1381 cggccggctg attctggccg gacgcgtcag cgcccgtagc tcactcccat gcccgcgcgc
     1441 acgtcgttgt tcacgccgta ttggggtatg gggtatcgaa ggccgttgcg ggatagctgc
     1501 tccagcccgg ccagcgtctt cggcaggaac attggcggca tcgccatgag cagctcgtct
     1561 tccatgacgc cgccgtagcg acgctcggca aagcatggga tggagaggct gggctcgccg
     1621 gtggccagcg cccggcccca cgagtccgcg caggcggact cgcccacgca tccccattcc
     1681 agcttgcggt aacccgacca ctgcagcccg ttgatgagca ggatcatctg ggccggcgtc
     1741 gcgtagatga ggcagatgtc gggaggatcg agcctgccgc tggccagcgg gctcacggcc
     1801 atggcctcgt agcgaccatg cggcaccacg tccatggcgt gctggtgagc gctggcatcc
     1861 tcgccggtct cgtaccaaac tccggtcatg cggtcgccgg agagccagtc gtcgctgcgc
     1921 ggatgcaggc cgatcaccgt gctgcactgg gcgccgacga ggtcctcggc ggtgatgccc
     1981 accgtccagc cgttgcgggc ggcctggccg acgatctgat ctgtcgtgtg aatgtcccgc
     2041 ggccgtcgga tgcgcggcac tgcctccatt tccccccgcg tctcgaacag cttcatcccg
     2101 atcggggtag tgcgcagccg caacagccgg ttcagaccgg ccaccagctc ggcccagtcg
     2161 tatgaggttt ggtccgtcgt tatcagatcg ctcatggggg gtcctcgtct tggcgcgttg
     2221 cgggagtcag tcctcgccca cgcgtttgaa tcgtggcggc ctgcgctcga tgaacgagcg
     2281 cacgccctcg gtgaagtcct cgcgcttgag gctctgggcc atccaggcat tgctctcgcg
     2341 catggcttcg cccagcgaca tgttgagatg ccggtacacc tgagccttca tcattttcag
     2401 ggaggtcggc gaggcgttgg cggccaggtc gcgtatgtag tcgcaggcct cgctgacggc
     2461 ggcgccctcg tcgaccaggc gttcggcgat gccgatacgc tcggcttccg tcccgtcgaa
     2521 ttttcgcgag ctccagagta tgtccagggc ccggccgggc ccgatcagcc ggggcaggat
     2581 ccagctcacc ccatgctcgg ctacgaggcc gcgctgggaa aaagcggtcg tgaacttggc
     2641 ctggggctcc acgaagcgca tgtcggtgag catggcaatg acgaagccga gccccgcgca
     2701 ggccccgccc accgccgcga tgacgggctt ctgcacggcc atgaggtagc cgtaggtcac
     2761 ctggaagtcc tcgcccatcc cggggtcccc cacctcggct tcgaggccgg agagatcttc
     2821 tgccgccgcc tcgccacccg aggacatacg gtccaggctg cccatgtcca tgccggcgca
     2881 gaatccgcgg cccgccccgg tcagcacgat gccgaccacg cggccgtcct tctctgccgc
     2941 tcccagcgcg tgccgaatct cggcgagcat ccggacggtg aaagcgttga ggcggtctgg
     3001 tcggttcatg gtgatgatcg cgaccggatc ctcgaccgtg taggtgattt cttcgtacat
     3061 gtggcgctcc ggagcgggcc gtttccgatg gaatgtaccg catcccgcga gccgagtaat
     3121 gcctggcggg ccttgacggc ggcgctgcac agcgggaacg atggtttatc atgtcggctt
     3181 ttggaaagtc tcgggtgcag tggtgagccg ctcgctatca tggttcctgg catttctggt
     3241 gctgctcggc gttctggccg ttgctggaag ctggtatctg ggccccagcc tggttgggct
     3301 ggcgttcgac gcggaccgtc gggaagcgcc ctactacgtt ctgagcctgg cgggccggag
     3361 cgagctggcc gacgcggatc tgagattcgg ctccgatctt gccgagctgt tcgacgcggc
     3421 cggtggcaag ctgctatggc gtggcaccac cgagcgggtc gtcaagggcc gtctgcagga
     3481 cgagtggctg aacgcccagc tgtttgcctt cccgcgtggc gcaaacttcg tggagctgaa
     3541 gacgggcggc gactaccgcg acctgctggc ggcctacgag ggcaggtcgc gtctgttgct
     3601 cggcagcccg acccaaccgg atcggatggc caggggccgt atctgggtcc tcaacctgat
     3661 cgcggaggat gccgaggact tgcaggcaac cggcggcgac cccctggctg gcttgatggg
     3721 cggcgtcgcg gacttcggcg gctccctggt ctgggatgcg aaggtcgcgg acctggacga
     3781 gcgctcgtcc tggaatcgtc tggtgctgct cggtttcgct caaccgctgg aggccgaagt
     3841 gtggctttcc gatgccggca cccaaaccga gctggcgctc agcatggagc gagcgcggcg
     3901 ccgggtcacc ctgctgatgc gcccggccga tccgatctag accctggccg aagggcggtg
     3961 aaatacccgc accgtgccga actcgtcgaa ctctccgagg ttcggaaacg tggccgcgtc
     4021 gatg
//
LOCUS       DBNHQR010000643         8579 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1391048, whole genome shotgun sequence.
ACCESSION   DBNHQR010000643 DBNHQR010000000
VERSION     DBNHQR010000643.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8579)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8579)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8579
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1391048"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(245..2587)
                     /gene="bamA"
                     /locus_tag="AC16VR_14700"
     CDS             complement(245..2587)
                     /gene="bamA"
                     /locus_tag="AC16VR_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003299362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HIR6012458.1"
                     /translation="MTGRGARIVVLLALLGSASLARADAFTISDIRLQGLQRVSAGTV
                     FNVLPVSVGDTIDEVSVRQLVRLLFRSGYFDDIRMARDGDILVVTFKERPAIESIEID
                     GNKAIKTDALLDGLGQQGLREGEIFKQATLERVGLELERQYVSQGRYGAAIDTQVEQL
                     PRNRVAIKIEIEEGKTSGIRHINIVGDEVFEQEELLDQLELQHPSLFSFYRNDDKYAR
                     EKLTADLEKLESYYRDRGYVEFDIESTQVSITPDRRQVYITVNIEEGDKYTVSGVNLI
                     GELSDVNPDDLKRLFLVAEGQVFSQALVTATEERLTQALGNSGYTFASASGVPRVSED
                     GTVEVVFNVDSGKRAYVRRVSFAGNKVTQDEVMRREMRQMEGGWASTAQIDFSKVRLE
                     RLGYFKGVSVETPEVPGTDDQIDVEFTVEEQPSGSVSATLGYSQGYGLIVGGNYQENN
                     VLGTGNSLGLGISYSDFQQSISFNYFNPYYTLDSISRGFNLFARRLDYDARNIARYST
                     DAYGGGITFGFPIGETQRINFGGIIEYTEITEGFAAAKEITEFIIKNGNTALNFKGNL
                     SWNSSTLNRGLFPTRGRSQSLALEVSIPGSDLQFYKVVYNAQAYFPMPFLRGWTIRAR
                     TELGFGDGYGKTEALPFFEHFYSGGYGSVRGFEQSTLGPRTTPVDNDFFIDDPDPFGG
                     NLLVEGSLELIFPLPFVEDSRQFRPVIFFDVGNVFNTQCPDVSEYCFGFDFGELRYSV
                     GMGVTWLTGMGPMTFSIAKAFNTGPEDETEGFQFELGRTF"
     gene            complement(2592..3926)
                     /gene="rseP"
                     /locus_tag="AC16VR_14705"
     CDS             complement(2592..3926)
                     /gene="rseP"
                     /locus_tag="AC16VR_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017479411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HIR6012459.1"
                     /translation="MDFLQYPLALLVTLGVLVTFHELGHFVIARWSGVTVVRFSVGFG
                     KPFWSWRDRRGTEFALAYIPLGGYVRMLDEREPGVIEYDRRSTDVSYNELSVWWRMAI
                     ALGGPAANFLLAIIVYWVLFVIGTITYAPLLGSVGADSAIGRAGLREHEELIAVDGVE
                     TSSWQAVNMALAGRLGESGEIELTTRRPGSDSVRSGAVPISSWHTGVDEPDLLGSLGI
                     VPALPPIVAQVVPDSPAERAGFRRWDRIVSVDGEAVADWGQWVDLIRNAPDRRLSVTV
                     DRSGQVLVLDVTPDVQEAAGGEVTGFLGVGAYTKEIRYGVLAAVPKSLWESWDKTALT
                     LGLLKKMITGHVSVKNLSGPITIAKVAGDSARSGWRFFFSVLALLSISLGVLNLLPIP
                     ILDGGHILFCGAEAVTGRPVSERVQILGTQIGLFMVASLMLLAIYNDISRLL"
     gene            complement(4047..4838)
                     /locus_tag="AC16VR_14710"
     CDS             complement(4047..4838)
                     /locus_tag="AC16VR_14710"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007949337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HIR6012460.1"
                     /translation="MLRTRILTALIIAPLAVAALYLLPASWFAVAYGLVAALGAYEWG
                     GLIGLRRFWSRLAYAALFVVAAGMTWWLDRGFGLAIVVGSIVWIVAAGLVLTYPATAA
                     VVREPWSNGLLGLFVLWGAWSAVVEVRGEPGSGGANWVMWLLVLVWSADVGAYFAGRR
                     FGRRKLAPAVSPGKTWEGAFGGMLVSLALCGGALLVLGLPAWWLLAVAGLVVVSIFGD
                     LFESVIKRVRGVKDSSNLLPGHGGILDRIDSLLAVLPVFALILAR"
     gene            complement(4919..5665)
                     /gene="uppS"
                     /locus_tag="AC16VR_14715"
     CDS             complement(4919..5665)
                     /gene="uppS"
                     /locus_tag="AC16VR_14715"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016392308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HIR6012461.1"
                     /translation="MEKVELDAPREEGGEAGCPRHVAIIMDGNHRWAKRRRLPGAAGH
                     RTGARNVRPVAEACADRGVTCLTLFAFSTENWQRPRQEVNLLMDLMRNVMENDLDELH
                     RREVRLQIIGDRSRFPSDLQMEMNRAEAMTRDNSRMSLNVAVNFGGRWDIAQAARALA
                     RQVREGECSLEEIDEERFAAHLSLGGLPDPDICIRTGGDHRISNFLLWDFAYTELYFT
                     DTYWPDFDAACLHEALNWFDDRQRRFGRRT"
     gene            complement(5706..6263)
                     /gene="frr"
                     /locus_tag="AC16VR_14720"
     CDS             complement(5706..6263)
                     /gene="frr"
                     /locus_tag="AC16VR_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HIR6012462.1"
                     /translation="MIEEIKQDANERMDKSLSSLQVNLSRIRTGRANPSLLDGIEVAY
                     YGNYTPLNQVASITVEDARTLVISPWEKNLVPDIEKAILKSDLGITPASTGDLVRVPL
                     PALTEENRRDLAKQAKQEAENARIAIRNIRRDAIQHIRELVKDKEAAEDEAHKAEDDV
                     QKLTDRHIDSIDEVLAAKEADLLEI"
     gene            complement(6260..6979)
                     /gene="pyrH"
                     /locus_tag="AC16VR_14725"
     CDS             complement(6260..6979)
                     /gene="pyrH"
                     /locus_tag="AC16VR_14725"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HIR6012463.1"
                     /translation="MQRLLLKLSGEALGGDVGFGIDPVVVRRLGEEISEVIAAGAEVA
                     LVLGGGNLFRGAALAEAGMDRITGDQMGMLATVMNGLAVRDLMRSGGVPAEVLSAIEI
                     PGVARKFTRDAAQELLRNGQVAIFVAGTGNPYFTTDTAACLRGIEIGADVVLKATKVD
                     GVYSADPMKVPGAERFDELTYDEVLRRDLKVMDSAAISLCRDNEMPLVVFAMAEAGAL
                     TRIVNGAKVGTRVVAVTDETA"
     gene            complement(7016..7885)
                     /gene="tsf"
                     /locus_tag="AC16VR_14730"
     CDS             complement(7016..7885)
                     /gene="tsf"
                     /locus_tag="AC16VR_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003092393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HIR6012464.1"
                     /translation="MAITAAMVKELRERTGLGMMDCKAALTEAGGDMELAIEELRKRS
                     ALKAAKKAGRTTADGLLGIKVAEDGSRGAIVEVNIETDFAAKNQKFVDFVGEVLAAVY
                     DGREDDVAKLVAGELEAKREALVQEIGENITVRRAALLGAGDGFVTFYLHGDNRKGTL
                     VELSSPNEELGRDLAMHVTAIAPMVVESSGVPADVLAKEREIYASQAEASGKPAEIVE
                     KMVEGRVRKFLAEVSLVDQPFVKDPDVKIGKLLSDAGVECRGFVRFEVGEGIEVEQTD
                     FAAEVAAQVSGAE"
     gene            complement(8111..8579)
                     /gene="rpsB"
                     /locus_tag="AC16VR_14735"
                     /pseudo
     CDS             complement(8111..8579)
                     /gene="rpsB"
                     /locus_tag="AC16VR_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S2"
BASE COUNT         1580 a         2882 c         2574 g         1543 t
ORIGIN      
        1 aggacggcaa tcttcagctc cgcccacgcc acacccgcgg tgagcgcgag aaagcacaac
       61 gaccaactgg caaacttctt aatcacaaca catctcctgc gtaattctgt cactcgtaga
      121 aagatacaac ccgtctaccg acccggctct aaaccgggcc gctgacgaaa catgtgacaa
      181 tcaattttcg cggccgttct gacggctctg ttatacgttt gtccgcaaag ccgcgcattg
      241 tacctcagaa cgtgcggcca agctcaaact gaaatccttc ggtctcatcc tcaggccccg
      301 tgttgaacgc cttggcgatg ctgaacgtca tcgggcccat gccggtcaac caggtaacac
      361 ccatccccac ggaatagcgg agttctccga aatcgaaccc gaagcagtac tcgctgacgt
      421 cggggcactg ggtgttgaat acgttgccga cgtcgaaaaa gatgaccggc cggaactgtc
      481 tgctgtcctc gacgaagggc agagggaaaa tcagctccag gctgccctcg acgagcaggt
      541 tgccgccgaa ggggtcgggg tcgtcgatga agaagtcgtt gtccacgggc gtcgtgcgcg
      601 gccccagggt gctttgctcg aacccgcgaa cagagccgta accgcccgag tagaagtgct
      661 cgaagaacgg tagcgcttcc gttttcccgt agccgtcacc aaagcccagc tccgtccggg
      721 cccggatggt ccagcctctc aggaagggca tggggaagta tgcctgggcg ttgtagacga
      781 ccttgtagaa ctgcagatcg ctcccgggta tcgaaacctc gagggccagc gactgcgatc
      841 gcccgcgcgt agggaagagc ccgcgattca acgtggagct gttccaggat agattgccct
      901 tgaaattcag cgcggtgttg ccgttcttga tgatgaactc ggtgatttcc ttggccgccg
      961 cgaacccctc ggtgatctcg gtgtactcga tgatgccgcc gaagttgatg cgctgagttt
     1021 caccgatggg aaagccgaac gtgatccccc ctccgtaggc atcggtggag tacctggcta
     1081 tgttgcgcgc gtcgtaatcg agccggcggg caaagaggtt gaatccccgg ctgatactgt
     1141 ccagcgtgta gtaggggttg aagtagttga agctgatgga ctgctggaag tccgagtagc
     1201 tgatgccgag cccgaggctg ttcccggtgc ctagcacgtt gttctcctga tagttgccgc
     1261 cgacgatgag accgtaaccc tgggagtagc cgagcgtcgc ggagacgctc cccgacggct
     1321 gctcttccac cgtgaactcc acgtcgatct gatcgtcggt ccccggaacc tcgggcgttt
     1381 ccacgctgac gcccttgaaa tagccgagcc gctcgagccg gaccttggag aagtcgatct
     1441 gcgccgtgga agcccagccc ccttccatct gccgcatctc tctgcgcatc acctcgtcct
     1501 gggtgacctt gttgccggca aaggaaacac ggcgcacgta tgcccgcttg ccggaatcca
     1561 cgttgaacac gacctcgacc gtgccgtcct cgctgacgcg aggtacgccc gaggcggatg
     1621 cgaacgtgta accggagttg ccgagggctt gggtgagccg ttcctccgtt gcggtgacga
     1681 gagcctgaga gaaaacctgc ccctcggcca cgaggaacag acgcttgagg tcgtcggggt
     1741 tcacgtcgct gagctcgccg atcaggttga cgccgctgac cgtgtacttg tcgccttcct
     1801 ctatgttcac cgtgatgtag acctggcgcc ggtccggcgt gatgctcacc tgggtcgact
     1861 cgatgtcgaa ctccacatag ccgcgatcgc ggtagtagga ctccagcttc tccagatcgg
     1921 ccgtgagctt ctcccgcgcg tacttgtcgt cgttgcggta gaaggaaaag aggctcgggt
     1981 gctgcaactc cagctgatcg agcagctctt cctgctcgaa gacctcgtcg ccgacgatgt
     2041 tgatgtgccg gatgccggaa gtctttcctt cctcgatttc gatcttgatg gccacacgat
     2101 tccggggcag ctgctcaacc tgggtgtcga tggcggcgcc gtaacggccc tgagaaacgt
     2161 actgccgctc cagctccagc ccgacccgct ccagggtcgc ctgcttgaag atttccccct
     2221 cccgcagtcc ctgctggccc agcccgtcga ggagcgcatc cgtcttgatc gccttgttgc
     2281 cgtcgatctc gatgctctcg atggccggcc gttccttgaa cgtgaccacc aggatgtcgc
     2341 cgtcgcgagc catgcggatg tcgtcgaaat agcccgacct gaacagcagg cgtaccagct
     2401 ggcggacgct cacctcgtcg atggtgtccc cgacgctcac ggggagcacg ttgaaaaccg
     2461 tccccgcgga cacccgctga agaccctgca aacgtatgtc cgatatggtg aaggcgtctg
     2521 cccgagccag acttgccgac ccgagcaggg ccagcagcac aacgattcgc gctcccctcc
     2581 ccgtcatcgt ttcagagcag cctggagatg tcgttgtaga tcgccagcag catcaggctg
     2641 gccaccatga acaggccgat ctgggtcccc agaatctgaa cccgctcgga gaccggccgg
     2701 ccggtaaccg cctccgcccc gcaaaacagg atgtggcccc cgtcgagaat gggaatgggc
     2761 agcaggttga gcacgcccag cgagatgctg agcagcgcga gaacgctgaa gaagaagcgc
     2821 caccccgagc gggccgagtc accggccacc ttcgcgatgg tgatgggccc actgagattc
     2881 ttgacggaca cgtgcccggt gatcatcttc ttgagcaggc caagcgtcag cgcggtcttg
     2941 tcccaggact cccagaggct cttgggaacg gcagcaagca cgccgtaccg gatctccttg
     3001 gtgtacgcgc ccaccccgag gaagccggtc acctcgccgc cggcagcttc ctggacgtcc
     3061 ggcgtcacgt ccagcaccag cacctgtccg gaacggtcta cggtcacgct caagcgccgg
     3121 tccggggcat tgcgtatcag atccacccat tggccccagt cggcgaccgc ctctccatca
     3181 acagagacga tccgatccca gcgacggaac ccggcgcgct ctgccgggct gtccggcacc
     3241 acctgggcga cgatgggggg cagtgcgggc acgatcccga gcgagccgag cagatccggt
     3301 tcgtcaacac cggtgtgcca gctggaaatc ggcacggcac cggagcgtac cgaatccgat
     3361 ccgggtctcc gggtagtgag ctcgatctcg ccgctctcgc ccagccgccc cgcgagcgcc
     3421 atgttcaccg cctgccagga ggacgtctcg acgccgtcca cggcgatgag ctcctcgtgc
     3481 tcgcgcagcc ccgccctgcc gatggcggaa tccgccccta cgctgcccag aagcggggcg
     3541 taggtgatcg taccgatcac aaagagcacc cagtagacga tgatggccag caggaaattg
     3601 gcagccggcc cgcccagagc gatggccatg cgccaccaga cgctgagctc gttgtaggag
     3661 acgtccgtgc tgcggcggtc gtactcgatg acgccaggct cccgctcgtc gagcattctc
     3721 acgtagccgc ccagcggaat gtacgcaaga gcgaactcgg tgccgcgccg gtcgcgccac
     3781 gaccagaaag gtttcccgaa gccgaccgag aagcggacga cggtgacgcc cgaccagcgc
     3841 gcgatgacga aatgacccag ttcgtggaaa gtcaccagga cgccgagagt caccagcagg
     3901 gcaaggggat attgcagaaa atccataaac tgagtgcgac agatgccccg tatcaaaact
     3961 tcagacagca gcggtgctcg ccggttcgcc ccgccctgga attacccaac cgtcctaaag
     4021 accatcggcg agcgcatcat aatggatcag cgagcgagaa tcagcgcgaa aaccggcagc
     4081 accgccagga gggagtcgat tctgtccagg atacctccgt gtccgggaag taagttgctc
     4141 gaatctttga cgccccgcac ccgcttgatc acgctctcga aaaggtcacc gaatatggag
     4201 accacgacga gccctgcgac cgcgagcagc caccaggctg gcagacccag caccagcaaa
     4261 gcgccaccgc aaagcgcgag gctgaccagc atgccgccaa aggccccttc ccaggtcttg
     4321 ccggggctta cggccggcgc cagcttgcgc cggccgaatc gccgccctgc gaaataggcc
     4381 cccacgtcgg cgctccagac cagcacgagc aaccacatga cccagttggc tccgccgctt
     4441 ccgggctcgc cacgcacctc tacgaccgcc gaccatgcgc cccagagaac gaacaggccc
     4501 aacaggccgt tgctccaggg ttcccggaca acggcagcgg tcgcggggta tgtcaatacc
     4561 aggcccgccg ccacgatcca gacgatggat ccgaccacaa tggccagccc gaaccctcta
     4621 tcgagccacc aggtcatccc ggcggccacc acgaacagcg cggcgtaagc cagacgcgac
     4681 cagaaccggc gcagcccgat gagccccccc cactcgtagg cgcccagggc agccaccagg
     4741 ccgtaggcaa cggcgaacca ggaggcgggg agcagataca gcgcagcgac ggcgagcggc
     4801 gctatgatca gggcagtgag gatccgggtc cgcagcacgg ctcagaccac tgtcggtcgc
     4861 atcggtgagg acggctgccg gggtagagct cgccgctcgt gcccgggcaa cgggctcgtc
     4921 atgttcgacg gccgaagcga cgctggcggt catcgaacca gttcagcgct tcatgcaggc
     4981 aggccgcatc gaaatccggc cagtacgtgt cggtgaagta gagttcggtg taggcgaaat
     5041 cccagagcag gaaattgctg atccgatgat ctccgcccgt ccggatacag atgtcgggat
     5101 cgggtagccc gcccagggag agatgggcgg caaaccgttc ctcgtcgatt tcctccaggc
     5161 tgcattcgcc ctcgcgaact tgccgcgcaa gggcgcgggc ggcctgggca atatcccaac
     5221 gcccaccgaa attgacggcg acgttgagcg acatgcgcga gttgtccctg gtcatggctt
     5281 ccgcacggtt catctccatc tgcagatccg agggaaaccg gctcctgtcc ccgatgatct
     5341 gaagccgcac ttcccggcga tggagctcat cgagatcgtt ttccatgacg ttgcgcatga
     5401 gatccatcag caggttcacc tcctggcggg gtcgctgcca gttctccgtg ctgaaggcga
     5461 aaagggtaag acaggtgacg ccccggtcgg cgcacgcctc ggccaccggc cgcacgtttc
     5521 gggcgccggt ccgatggcct gccgcacccg gcaaccgccg ccgttttgcc caccgatggt
     5581 taccatccat gatgatggcc acgtgccgag ggcaaccggc ttccccgcct tcctcgcggg
     5641 gggcatctaa ttcgactttc tccaagcctg gcgtacgttc tgccatggtg ttccagctcg
     5701 cccggtcaaa tttccagtaa atcggcttcc ttggccgcga gcacctcgtc gatggaatcg
     5761 atgtgccggt cagtcagctt ctgaacgtcg tcttccgcct tgtgcgcttc gtcctcggca
     5821 gcttccttat ccttgaccag ctcgcgaatg tgctgaatcg cgtcccgccg gatgttgcga
     5881 atggcgatcc gggcattttc agcctcctgc ttcgcctgct tggcaaggtc tcggcggttt
     5941 tcctcggtga gtgccggcag cggcactcgc accagatcac cggtggatgc gggcgtgatc
     6001 cccagatcgc tctttaaaat ggccttttca atatccggga cgaggttctt ctcccagggc
     6061 gaaatcacca gcgtgcgggc atcttcgacg gtaatcgagg ccacctggtt caaaggggtg
     6121 tagtttccgt agtaagcgac ctcgatgccg tcgagaaggc tcggattggc acggccggtc
     6181 cggatccgcg aaagattcac ctgcagcgac gacaacgact tgtccatgcg ctcgttcgca
     6241 tcctgcttga tttcttcgat catgccgttt cgtccgtgac cgccacgact cgggtgccaa
     6301 ccttggcacc gttgacaatc cgggtcaatg cgcccgcctc ggccatggca aaaaccacca
     6361 acggcatctc attgtcgcgg caaaggctga tggcggcgct gtccatgacc ttgaggtcgc
     6421 gccgcaacac ctcgtcgtag gtgagctcgt cgaaccgctc cgcgccgggc accttcatcg
     6481 gatcggcgct gtaaaccccg tcgaccttgg tcgccttcaa aaccacgtcg gcaccgatct
     6541 cgatgccgcg caggcaggcg gccgtgtcgg tggtgaagta cgggttgccc gtcccggcaa
     6601 cgaatatggc gacctgtccg ttgcggagca gctcctgcgc cgcatcacgg gtgaacttgc
     6661 gagcgactcc cgggatctcg atggccgaga gcacctcggc cggcaccccg ccggaccgca
     6721 tgagatcgcg aacggccaga ccgttcatta ccgtggccag catgcccatc tgatcgccgg
     6781 tgatgcggtc cattcccgcc tccgccagcg cggcgccgcg aaacaggttg cccccgccga
     6841 gaaccagcgc gacctcagcg ccggcggcga tgacttccga aatttcctcg cccagccggc
     6901 gcaccacgac gggatcgatg ccaaagccca cgtcgccccc aagagcctcc ccgctgagct
     6961 tcagcagcaa tctctgcaat gctatgacct ccggcccgcc gaggctggct taggtttatt
     7021 cggcaccgct gacctgggcg gccacctcgg cagcgaaatc ggtctgctcg acctcgattc
     7081 cctcgcccac ttcgaagcgc acgaagccgc ggcattcgac cccggcgtcg ctcagcagct
     7141 tgccgatctt gacgtcggga tccttaacga acggctggtc gaccaggctc acctccgcga
     7201 gaaacttgcg cacccgtcct tcgaccatct tttcgacgat ctcggccggc ttgccgctgg
     7261 cctcggcctg ggaggcatag atttcccgtt ctttcgcgag cacgtccgcc ggcacgcctg
     7321 acgactcgac aaccatcggg gcgatggccg tgacgtgcat cgccagatcg cgaccgagct
     7381 cctcgttggg cgaggaaagc tccacgagcg tccccttgcg gttgtcgcca tgaaggtaaa
     7441 acgtgacgaa cccgtcaccc gcgcccagca gggccgcgcg gcgcaccgtg atgttctcgc
     7501 cgatttcctg gaccagggct tcgcgcttgg cctcgagctc gccagccacg agtttggcca
     7561 cgtcatcttc gcggccgtcg taaacggccg ctaagacctc gccgacgaag tcaacgaact
     7621 tctggttttt ggccgcaaag tcggtttcga tgttgacctc gacgatggcg cccctggaac
     7681 catcctcggc aaccttgatg ccgagaagcc cgtccgccgt cgtgcggccg gctttcttgg
     7741 cagccttcaa ggcgctgcgc ttacgcagct cctcgatggc cagctccatg tctcccccgg
     7801 cctcggtcag cgcggctttg cagtccatca tgccaagccc ggtacgctcc ctgagttcct
     7861 tgaccattgc tgcggtgatc gccattactc aagcctccgt cggtttgttc tcggcatcgg
     7921 cctctgccga ctctgcagct tcgtccgaac cagtctcggc agcgccggtc tccggctcgg
     7981 ctgcaacgtc tcccgcctga gcttcggcgg tcgtggcagc ctcaggctcc gccgcgggag
     8041 ccgagggagc cgcggcagcc gcaggagcag tcgcggacgc agtcgcggac gcagtcgcgg
     8101 gcgcagtctc aggcgcttcc tccacctgct ccgcctcgac ctcgacgaat tcgccggggc
     8161 gtacggcacc ggccgcgttg tcgacgcctt cttgaatggc atccgccacc gcggtgacgt
     8221 agagccggat cgcccggatg gcatcgtcat ttccggggat gacccagtcg acgccgtcgg
     8281 ggtcgctgtt ggtgtccacg acgccgaaga cggggatccc gagcttgttc gcttcgctga
     8341 tggcgatgtg ctcgcgcatc acgtcgatga cgaagatcgc gtccggcagc ccggccatct
     8401 ccttgatgcc accgaggctc cgctccagct tctccatcag gcgccggcgc tgcagcgcct
     8461 cgcgcttggt gagcagctcg aaggtgccgt caacggcctg agattccagc tcctgcagcc
     8521 gcttgatgga ttgccggatg gtcttgtagt tggtcagcat gccgccgagc cagcgctgg
//
LOCUS       DBNHQR010000644         3156 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_633519, whole genome shotgun sequence.
ACCESSION   DBNHQR010000644 DBNHQR010000000
VERSION     DBNHQR010000644.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_633519"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="AC16VR_14740"
     CDS             <1..1063
                     /locus_tag="AC16VR_14740"
                     /EC_number="5.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HIR6012465.1"
                     /translation="RPESLHRTARYIASVQQNDGAIPWFAGGITDPWDHVEAAMGLSI
                     GGHHAQAAAAYRWLARMQRADGSWLAAYSGARVADGTRAETNFVAYVATGTWHHYLIS
                     EDGKFLRSMWPTVKRALDFVTGLQAPTGEIYWAQDTRTGINKDALVTGCSSIYKSLEC
                     GIFMAAELNEDPGAWPACRARLGDAIILRPDRFDRTWESKSRYSMDWFYPVLAGLFSQ
                     ADARERLDERWETFVAPSLGCRCVSDQAWVTVAESCELAIACVASGEPDKAEELCDDL
                     ARFQAEDGSWWTGYAFEDDALWPDERPTWTAGAVLLAADAMHGLTPASALFTDNALPD
                     APEHSERAGRFQRLEQARG"
     gene            complement(995..1609)
                     /locus_tag="AC16VR_14745"
     CDS             complement(995..1609)
                     /locus_tag="AC16VR_14745"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6012466.1"
                     /translation="MKLPLDPDEIKGFLHPEEGARLYALASVASGLGPCLEVGSYCGK
                     STLYLGTACRDQGQVLYAVDHHRGSEEHQPGEAYHDPELFDAEIGSMESLMAFRRTMY
                     RAGLEGCVIPVVAASALAGRHWATPLALVFIDGGHSVEAAQTDYRTWAPHVVPGGFLA
                     IHDLFPDPLEGGQAPIDVYRLASASGLFEPLETTRTLGVLRRVG"
     gene            1732..1863
                     /locus_tag="AC16VR_14750"
     CDS             1732..1863
                     /locus_tag="AC16VR_14750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012467.1"
                     /translation="MHDENEREPVEELFAAEAPFAFPDQLYNEDDAEDNLSYVRGYN"
     gene            complement(1882..2070)
                     /locus_tag="AC16VR_14755"
     CDS             complement(1882..2070)
                     /locus_tag="AC16VR_14755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012468.1"
                     /translation="MAGVILTLILAFVAGGALWIGIGPRLKLAEDAERNGLLNLLAYV
                     AIALPVAFAVVFYLLELI"
     gene            complement(2110..2982)
                     /locus_tag="AC16VR_14760"
     CDS             complement(2110..2982)
                     /locus_tag="AC16VR_14760"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6012469.1"
                     /translation="MTYSFEGKVAIVTGAGGGLGRCHALELARRGAKVVVNDLGGAMD
                     GTGGSSAAADEVVAEIKAGGGEALANGGSVSDRQGAKSMVVDAMNAWGRVDVLINNAG
                     ILRDRSFGKMELDDFQIVVDVHLMGAVNVTREVWPIMKEQNYGRIVMTTSPTGLYGNF
                     GQTNYGAAKLGQVGFMNSLKIEGAKNNIYTNTIAPVAATRMTEGLIPEGSQVALGPEL
                     VTPAVIFLCSEEAPNGVIVQAQGGNYSVACIVENEGVNLGLDATAEDVGANFEKISDL
                     TGAKPRGMLQLSSM"
BASE COUNT          581 a         1065 c          964 g          546 t
ORIGIN      
        1 gcggccggaa tctctgcacc gcaccgcccg atacatcgcc tccgtccagc aaaacgacgg
       61 cgcgatcccc tggtttgcgg gagggatcac ggatccctgg gaccatgtgg aggccgccat
      121 ggggctgagc atcggcgggc atcacgctca ggccgcggcc gcttaccgct ggcttgcccg
      181 gatgcagcgg gccgatggtt cctggctggc tgcctacagc ggtgcgcgcg tggcggacgg
      241 cacccgagcg gagaccaatt tcgtcgccta cgtggcaaca ggcacctggc atcattatct
      301 gatctccgag gacggcaagt tcctgcgctc catgtggccg acagtgaagc gcgcgcttga
      361 tttcgtcacc ggcctgcagg ctcccacggg cgagatctac tgggcccagg atacccgcac
      421 gggcatcaac aaggacgccc tggtcaccgg ttgcagctcc atctacaaga gcctcgagtg
      481 cggcatcttc atggccgcgg agctcaatga ggacccgggc gcctggcccg catgccgggc
      541 gcgactcggc gatgccatca tcctgcgccc cgatcgcttc gaccgcacgt gggaaagcaa
      601 gtcccgctac tccatggact ggttctatcc ggtgctggcg gggctctttt cacaagccga
      661 cgcacgcgaa cggctggacg agcgatggga aaccttcgtc gccccttcgc tgggctgccg
      721 ctgcgtctcc gaccaggcct gggtcacggt tgccgagtcc tgcgagcttg ccatcgcctg
      781 cgtcgcttcc ggcgagccgg acaaagccga ggagctctgc gacgacctgg cgaggttcca
      841 ggccgaggac ggttcttggt ggacgggata cgcgttcgag gacgacgccc tgtggcccga
      901 cgagcggccc acctggaccg ccggggccgt gctgctggcc gccgacgcca tgcacgggct
      961 gaccccggca tcggccctgt tcacggacaa tgcgctaccc gacgcgccgg agcactccga
     1021 gcgtgcgggt cgtttccaga ggctcgaaca ggccagaggc tgacgccagg cggtagacgt
     1081 ctataggcgc ctggccgcct tcgagaggat ccggaaacag atcgtggatg gccaggaaac
     1141 cgccgggcac gacgtgggga gcccaggtcc ggtaatcggt ctgagcagct tcgacgctgt
     1201 gcccaccgtc aatgaacacg agcgcaagcg gcgtcgccca gtggcggccc gccagcgcag
     1261 acgccgcaac aaccgggatc acgcatcctt ccagacccgc gcggtacatg gttctgcgaa
     1321 acgccatcag cgattccatg ctgccgatct cggcatcgaa gagttccgga tcatgatacg
     1381 cctcacccgg ctggtgctcc tccgatcccc gatggtgatc gaccgcgtag agcacctggc
     1441 cctggtcccg gcaggccgta ccgagataaa gcgtggactt gccgcagtag ctgcccacct
     1501 cgaggcaggg ccctaggccc gacgcaacgc tggcgagcgc gtagagccgt gcgccttcct
     1561 ccgggtgaag gaaccctttg atctcgtcgg gatcgagtgg aagcttcatg tcgaaaaacc
     1621 gagcttttcg tcgcaattca gcgcaaatcc gacggcaacc tcgcagaacg ggattgcaat
     1681 ttcaacatct gacactagac tcccctcaca cgaccctgcc ggttcaacaa gatgcacgac
     1741 gaaaacgagc gagagcccgt cgaggagctg ttcgcggccg aggcaccctt tgccttcccg
     1801 gaccagctct acaacgagga cgacgcagag gacaatctca gctacgtccg cggctacaac
     1861 tgattcagcc ctcgcgccag ctcagatcag ctccagcagg tagaacacca ccgcgaacgc
     1921 caccggcaaa gcgatggcga cgtatgcgag caggttgagc agcccgttgc gctcggcgtc
     1981 ctcggcgagc ttcagcctcg ggccgatgcc aatccatagc gcgccgccgg caacgaatgc
     2041 cagaatgagc gtgagtatta cccctgccat atgtgcccgt cggcgcgctg caaagatcct
     2101 gccgccagcc tacatgctgc tgagctgcag catgccccgt ggctttgccc cggtgagatc
     2161 tgagatcttc tcgaagtttg ctcccacatc ctctgcagtg gcgtccaatc ccaggttgac
     2221 gccttcgttc tcgacgatgc aggccaccga gtagttcccg ccctgagcct gaacgatcac
     2281 gccgttgggc gcttcttcgc tgcaaaggaa gataacggcc ggggtcacga gctccggacc
     2341 gagcgcaacc tgcgagccct cgggaatgag tccttccgtc atccgcgtgg ccgccaccgg
     2401 cgcgatggtg ttggtgtaaa tgttgttctt ggcgccctcg atcttcagcg agttcatgaa
     2461 acccacctga ccaagcttgg ctgcgccgta gttggtctgc ccgaagtttc cgtaaagacc
     2521 cgtgggcgag gttgtcatga cgattcgccc gtagttctgt tccttcatga tcggccacac
     2581 ttcgcgggtg acgttgacgg cacccatcag gtgcacgtcg acgacaatct ggaagtcgtc
     2641 cagctccatc ttgccgaagg agcggtcgcg cagaatgccc gcgttgttga tcagaacgtc
     2701 cacgcggccc catgcgttca tggcatcgac gaccatgctc ttcgcaccct gccggtccga
     2761 tacggacccg ccattggcaa gggcctcgcc gcccccggcc ttgatctcgg cgacgacctc
     2821 gtctgccgcc gcagacgacc ccccggttcc gtccatggcg ccaccgaggt cgttgaccac
     2881 gaccttcgcc cctcgccggg ccagctcgag ggcgtggcat cggccaagac cgccgccggc
     2941 tccggtcacg atggcaacct taccttcaaa cgaataagtc atattcttct ccagggctgt
     3001 agtcagctaa gcaaacgaga gagccgcgca ttctaatgca atctgggcgc cgggtgatca
     3061 gggccggata ccgaaacgaa cggggccggt gaggttgtcc agacgcggct ggatccaatc
     3121 gacccactcg cacagcatcg gacgcgtctc ccgggg
//
LOCUS       DBNHQR010000645         6224 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1582769, whole genome shotgun sequence.
ACCESSION   DBNHQR010000645 DBNHQR010000000
VERSION     DBNHQR010000645.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6224)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6224)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6224
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1582769"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="AC16VR_14765"
     CDS             complement(<1..254)
                     /locus_tag="AC16VR_14765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012470.1"
                     /translation="MTCPRQGFAKLSEARLGLEQAAPDSITKLDAIRHSVEARLRELS
                     PSAAEGGERIEHAIQYSLLDGGKRLRPLLTILTASTFDADP"
     gene            complement(278..1813)
                     /locus_tag="AC16VR_14770"
     CDS             complement(278..1813)
                     /locus_tag="AC16VR_14770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR6012471.1"
                     /translation="MINPATPRAAGVWPFCVAAKVLVLVIAGTQYGFLADELYLLDAA
                     ARPAFGYVDFPPLTPWLLALLGWIGGTDLIWLRGLACAAGIVTTLVAVDVCRILGGRN
                     FAQWTTAIVVLFAPAFMSVQSIFTMNVLDQLWWLLALSVLLRYLHDHQPRYMILLGVI
                     FGLALLTKLAIAAFILCLLGTSALYARWMFYRTESWAGSAIALSMLAPFVMWQAVSGW
                     PLLEFVQAYNVSEPEPMVLDQPLFGLIATMNPPFLLIWLPGLIYSLMAPKPIRVVGSA
                     AVATLVFFLAAGVKFYFATPLFALFTALGAVLWQRWLSSSGARSCLLAILLISGVAAV
                     PIAAPVLAPTTLQQVANFIRDAEQDKVADDDAPIGRYFPHFAEMHGWPELTEWVTRAY
                     EGLPSARRPEVAVVAAYFGQAGALNQLDKADRLPVVHSGHMNYYLWWQADEPNARLED
                     VLLVGFHPKELEPLFAEITILDRFSCNRCMTREQGAYLIRARRPLIDPEEIRDRLKRF
                     HFF"
     gene            complement(1810..2871)
                     /locus_tag="AC16VR_14775"
     CDS             complement(1810..2871)
                     /locus_tag="AC16VR_14775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR6012472.1"
                     /translation="MPTFNEADNVGMVVERLDACLGDHRWEVIFVDDDSPDETAGEVR
                     RLAQSDGRVRCVQRLNRRGLSSACIEGMLASSAPYIAVMDGDLQHDESLLPGMLNALK
                     ADDLDIVVGSRYVSGGDAEAFTRDRARKSRVATRLSRMVVPDELRDPMSGFFMIRREA
                     FEASMRQLSGIGFKIMMDLFASSPGPMRFREFPYSFRTRSQGESKLDNQALWDYGMLL
                     LDKLFGRYVPVRFVSFMLVGGFGVLVHLAVLFVLYQSLSLSFLVSQTSASLVAMTANF
                     FMNNVITYRDQRLRGWEWLGGWFSFVLACSVGALANVGIATYLLDRGTPWSLAGFTGI
                     LVGAVWNYAITQIYTWRAR"
     gene            3009..3506
                     /locus_tag="AC16VR_14780"
     CDS             3009..3506
                     /locus_tag="AC16VR_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR6012473.1"
                     /translation="MLLLVALVIAALDPLELRAETAVLLDQFGVPGSLAENAGSVQVV
                     IVVSAKRLRRIKPWERALREQFGDLPVLRVADLPPTSSATHEQVAGKLAKRLPPDLSV
                     LIDLEGRWAGTHGLDTSVPNLLLFAGNGQLIAREAGMFSASGFAEFATRVQDALDGGA
                     EQAVP"
     gene            complement(3503..4729)
                     /locus_tag="AC16VR_14785"
     CDS             complement(3503..4729)
                     /locus_tag="AC16VR_14785"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6012474.1"
                     /translation="MAIFHAMVLGLLRDRGALLMSFFLPAVFFVIMAEIFTTTSGESL
                     QLNVVVKDELDDEVSGRLLSALQATPTLTLTSDPDLDRDAVRALVKSGAYDIGLIVRA
                     DGEPLDSAGGFGAAPLLIVSDPARGVAIPMLAGQIRRAYFSALPDVAMGNVIGELENQ
                     FLTLTEAQHEAVAEGLLEMRSEAEAGREVGWSFEDLLEQESVAGGESVNLVAYSAGAV
                     AFLFLLFASVHGAVSLLEEREAGILDRVLAGPGGISIMVNGKLIYLVVQGIVQVGVIF
                     MAAWLIYDVDVPANLVEWLVVTLAASLAAAGLAMLLATACTTRRQAQTVANTVILVIS
                     ALGGSMVPRFFMPEALRDLGWLTPNTWALEAYSGIFWRQEPLTHLALPIGLLAGIGVL
                     GWQAALFLARRRAYGP"
     gene            complement(4732..5691)
                     /locus_tag="AC16VR_14790"
     CDS             complement(4732..5691)
                     /locus_tag="AC16VR_14790"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6012475.1"
                     /translation="MPTATEQTTPPLLQLDGVRVDLDGRSVLNDVSMSVAGGEVVVLL
                     GPNGAGKTTLLSLACGRRAPDAGRVRLAGHDPRTESSVRRQLGFVPQHVALYQHLSVV
                     ENLSVLGRMMGVARGDLAGRVAEALDWSGLADRARDRVGTLSGGMRRRLNIVASLLHE
                     PALLLLDEPTVGVDVAARERVHELLRRQRSQGLGILLTTHDLPQATELADRVVMLVDG
                     SVRLSGVPGKLIQDTFADKRELTVTLAQPATDPQSRTLRDAGLTPGRGGLTWYGAADN
                     EHADARRWDDRLQEMALAIAEIRVRAPTLEGVYLRLTGEELDL"
     gene            complement(5745..6038)
                     /locus_tag="AC16VR_14795"
     CDS             complement(5745..6038)
                     /locus_tag="AC16VR_14795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012476.1"
                     /translation="MEIVNHAREQLGPLTRQLLDAIDTESHPMEASFFAGLNVRLQHM
                     NDEGDLAELFFELSSTAFRGFAFSDHQAALIDRLLRAAEEIAFALSAPDDNAH"
BASE COUNT         1187 a         1967 c         2060 g         1010 t
ORIGIN      
        1 gggtctgcgt cgaatgtcga agcagtcagg atggtgagca gcggcctgag ccgcttgccg
       61 ccgtccagca ggctgtattg gatggcgtgc tcgatgcgct cgccgccttc ggcagcggac
      121 ggcgaaagct cgcgaaggcg cgcttccacc gagtggcgga tcgcgtccaa cttcgtaatg
      181 ctatctggcg ccgcctgctc caggccaagt ctggcttcac tcaatttggc aaaaccttgt
      241 ctgggacagg tcattttgcc tacatctgcc cggcgactca aaagaaatgg aagcgcttga
      301 gcctgtcgcg gatctcttcg ggatcaatca gcggccgtct tgcgcgaatc agataggcgc
      361 cctgttcccg cgtcatgcag cgattgcagg agaagcggtc gaggatggtg atttccgcaa
      421 agagcggttc gagctccttt gggtggaagc cgacgaggag cacgtcctcg agccgggcat
      481 tcggctcgtc cgcctgccac cagaggtagt agttcatgtg gccggaatgc acgacgggca
      541 gacggtcggc cttgtccagc tggttgaggg cgccggcctg gccgaaatac gcggcgacca
      601 cggcgacttc gggtctccgg gcggagggca ggccttcgta ggcccgggtc acccattcgg
      661 tcagctcagg ccatccatgc atctccgcaa agtgcggaaa gtatcgcccg atgggtgcgt
      721 catcgtctgc gactttgtcc tgttcggcgt cccgaatgaa gtttgccacc tgctgaagcg
      781 tggtgggcgc gagcacgggg gcggcaatcg gaacggccgc gactccggag atcaggagaa
      841 tggcgagcag gcagctgcgg gcgcccgagc tggaaagcca gcgctgccag agcacggcgc
      901 ccaaggcggt gaacagcgcg aacagcggcg ttgcaaagta gaacttcacc ccggcggcga
      961 gaaagaaaac cagggtcgcc accgctgcgc tcccgaccac ccgtatgggc ttcggagcca
     1021 tcagcgagta aatcagaccc ggaagccaga tcagcaagaa cggggggttc atggtggcga
     1081 tgaggccgaa gagaggctga tcgagaacca tgggctccgg ttcgctgaca ttgtaagcct
     1141 ggacgaactc cagcaacggc caccccgaaa ccgcctgcca catcacgaac ggggccaaca
     1201 tgctcagagc tatggccgac cccgcccagg actccgttcg gtagaacatc catcgtgcgt
     1261 acagggcgct ggtgcccagc aggcacaaga tgaacgcggc gatggcaagc ttggtgagaa
     1321 gtgcgaggcc gaagatgacg cctagcagga tcatgtagcg gggctgatgg tcatgcaggt
     1381 agcgaagcag cacagacaat gcaagcagcc accagagctg gtcgaggacg ttcatcgtga
     1441 agatcgactg gaccgacata aacgccggag cgaagagcac gacgatggcc gtggtccatt
     1501 gggcgaagtt gcggccgccg aggatccggc acacgtccac cgccaccaac gtggtgacga
     1561 tgccggcggc gcaggcaagc cctcgcagcc agatgagatc ggtgccgccg atccagccga
     1621 gcagggcgag cagccatggc gttaggggcg ggaaatcgac atagccgaaa gccggccggg
     1681 cagcagcgtc gaggaggtag agctcgtcgg ccaggaaacc gtactgggtg ccggcaatca
     1741 ccaggacgag aactttggcc gcaacgcaga aaggccagac acccgcggct cggggcgtcg
     1801 ccggattgat caccgagcgc gccaggtata gatctgcgtg atggcgtagt tccagacagc
     1861 gcccaccaga atccccgtga agccggcgag tgaccaggga gtcccacgat cgagcagata
     1921 ggtcgcgatg cccacgttgg ccagggcgcc aacgctgcag gcgagcacga aggaaaacca
     1981 gccccccagc cattcccagc ctctgaggcg ctggtcgcga taggtgatca cgttgttcat
     2041 gaaaaagttg gcggtcatgg cgaccagcga agcggacgtc tgactgacga gaaagctcaa
     2101 cgacagggac tggtacagaa cgaacagcac ggccaggtgc accagcacgc caaagccgcc
     2161 gaccagcatg aaggagacga agcgcaccgg aacgtagcgt ccgaacagct tgtccagcag
     2221 cagcatcccg tagtcccaca acgcctggtt gtccagcttg ctttcaccct ggcttcgggt
     2281 gcgaaagctg taggggaact cgcgaaagcg catgggcccg ggtgacgacg caaacaggtc
     2341 catcatgatc ttgaacccga tgccggagag ctggcgcatg ctcgcttcaa aggcttcgcg
     2401 ccggatcatg aagaagccgc tcatgggatc tcgcagctcg tctggaacga ccatccggct
     2461 cagccgcgtt gcgacccggc tcttgcgggc cctgtcgcgg gtgaaggcct cggcgtcacc
     2521 tccgctgacg tacctgctgc ccaccacgat gtcgagatcg tctgctttta gggcgttcag
     2581 cattcccggc agcaggctct cgtcgtgctg cagatcgccg tccattaccg cgatgtacgg
     2641 cgcgctgctg gcgagcatgc cctcgatgca cgcggaggag agaccccgtc ggttcagccg
     2701 ctgcacgcag cggacgcgtc catcgctctg agccagccgc cggacctcac ccgccgtttc
     2761 gtccggtgag tcgtcgtcca cgaagatcac ctcccagcga tgatctccca ggcaggcgtc
     2821 gagccgttcg accaccatgc cgacattgtc ggcttcattg aaggtcggca cgacgacaga
     2881 aagctctacg gccggtgccc gcaaatgttc ccccgtggat tcgcgttagg ttcgctcatg
     2941 gcgaaaggct aaactggcga tgacgaagat cctggattct ggccggtttg tccaaccgtt
     3001 ttgggtacgt gctgctactc gtcgcgctgg tgatcgcggc cctcgacccg ctggaactcc
     3061 gggccgagac cgcggtgctg ctcgatcagt tcggcgttcc gggatcgctg gcagagaacg
     3121 ccggctccgt tcaggtggtc atcgtcgttt cggccaagcg attgcgccgc atcaagccct
     3181 gggagcgggc cctgcgggag cagtttggcg acctgccggt gctgcgcgtc gccgatttgc
     3241 cacccacgtc gtccgccact cacgagcagg tggcgggcaa gctcgcaaaa cgtttgccgc
     3301 ctgacctttc ggttctcatc gatctcgagg gccgctgggc cggcacccac gggctggata
     3361 ccagcgtccc caatctgctc ctgttcgccg gaaacggaca gctcatcgcc cgggaagcag
     3421 gcatgttcag cgcatccggc ttcgccgagt tcgccacgcg cgtgcaggac gcgttggacg
     3481 gaggcgcgga gcaggccgtg ccctaggggc cgtaggcgcg acgccgggcg agaaagagcg
     3541 cggcctgcca accgaggact ccgattcccg ccagaaggcc gatgggcagc gcgaggtgtg
     3601 tcagcggttc ctgacgccag aaaatgccgc tgtaggcttc cagcgcccag gtattggggg
     3661 tgagccagcc gaggtcacgc agcgcttcag gcatgaagaa gcggggcacc atgctcccac
     3721 ccagggcgga aatgaccagg atgacggtgt tcgccactgt ctgagcctga cggcgggtgg
     3781 tgcaggcagt tgcgagcagc atggctaagc ccgcggccgc cagggaggcc gccaacgtga
     3841 cgaccagcca ctcgaccagg ttcgcgggca catcgacgtc gtagatcagc caggccgcca
     3901 tgaagattac gcccacctgt acgatcccct ggacgacgag gtaaatcagc ttgccgttca
     3961 ccatgatgct gatgccgcct ggacccgcga gcacgcgatc gaggataccg gcctcgcgct
     4021 cctcgagcag cgaaacagcc ccatggacgg atgcgaagag caggaagagg aacgccaccg
     4081 cgccggcgga ataggcgacg aggttaacgg attccccgcc ggcaacgctc tcctgttcca
     4141 gcaggtcttc gaaggaccag cccacctctc ggccggcctc ggcctccgaa cgcatttcca
     4201 gcagaccctc tgctaccgcc tcgtgctggg cttcagtgag tgtcaggaac tggttttcca
     4261 gctccccgat gacgttgccc atggccacgt cgggcagagc cgagaaatag gcgcgcctga
     4321 tctgacccgc cagcatgggg atggccacgc cacgggccgg atcgctgacg atcagaagcg
     4381 gcgccgctcc gaacccgccg gcggagtcca gcggctctcc atcggcacgg acgatcaggc
     4441 cgatgtcgta cgcgccggac ttcacgaggg ctctgaccgc gtcccggtcg aggtcagggt
     4501 cgctggtcag agtcagtgtg ggggttgctt gcagggcgga gagaagcctg ccggacactt
     4561 catcgtccag ctcgtccttt acgacgacgt tgagctgcag gctctccccg gacgtggtcg
     4621 tgaagatctc ggccatgatc acaaaaaaaa ccgcgggcag gaaaaaactc atcagcaggg
     4681 cccctcggtc acgcagaagg cccaacacca tggcatgaaa gatcgccatg ctcacaggtc
     4741 gagctcctcg cccgtcaggc gcagatagac gccctctaga gtcggggcgc gaacgcggat
     4801 ctccgcaata gcgagcgcca tctcctgcag tcggtcgtcc caacggcggg catcggcgtg
     4861 ttcgttatct gcagcaccgt accaggtgag cccaccgcgg cctggggtca ggcccgcgtc
     4921 gcgcagcgtc cgggactggg ggtccgttgc cggttgcgcc agggtgaccg tcagctcgcg
     4981 tttgtcggcg aaggtgtctt ggatcagctt tcccggcacg ccggacagac gaacggatcc
     5041 atcgaccagc atgacgacgc ggtcggcgag ctcggtcgcc tggggaagat cgtgagtggt
     5101 cagcagaatt cccaggccct gggatctctg ccggcgcagc agttcgtgca cgcgctcccg
     5161 ggcggcaaca tccacgccca cggtgggctc gtcgagaagc agcagagccg gctcgtggag
     5221 caggctggcg acgatgttca gccgccgacg catcccgccg gacagggttc cgaccctgtc
     5281 ccttgcgcga tcggcaagcc cgctccagtc gagggcttcg gcgacccggc ccgccaggtc
     5341 gccgcgcgcc acccccatca tccggcctag aacgctgagg ttttccacta cggacaggtg
     5401 ctggtacagc gcgacgtgct ggggcacgaa cccgagctgc cggcgcaccg aactttcggt
     5461 tcgcgggtcg tggcccgcca gccgaacccg gccggcatcc ggagcgcggc ggccacaggc
     5521 cagggagagg agggtggtct tgcccgcgcc gtttggcccc agcagcacca cgacctcgcc
     5581 gcctgccacg ctcatcgaaa cgtcgttcag cacgctgcgt ccgtccaagt cgacgcgtac
     5641 accgtccagc tgcagaaggg gaggcgtggt ctgttcggtg gcggtgggca cgatgtgggc
     5701 atcatgccac tgaacggtag caggcgaaat tggggcccgg cgttctagtg agcgttgtcg
     5761 tccggtgcgc tgagggcgaa ggcgatctct tcggcggcgc gcagcagccg gtctatcagg
     5821 gcggcctggt ggtcggagaa ggcgaaaccg cgaaacgccg tgctggaaag ctcgaaaaag
     5881 agctcagcca gatcaccctc gtcgttcatg tgctgaagcc ggacgttcag gccagcgaaa
     5941 aacgaggctt ccatggggtg cgactcggtg tcgatggcgt cgagcagttg ccgcgtcagc
     6001 ggcccgagtt gctcacgggc atggttcacg atttccatgg tgcgatgctg gcggtggcgg
     6061 ggtgaaattg acgtcaaaac ctcggtgagg gccgtggccg ctctgcgctt gccggatgcc
     6121 gccggccgcg ccttgccggc cgcgcctcgc cggccgcgcc ttgccggccg cgcactttca
     6181 cggctttttt atcggaatct agataacatc gggttcttgc tacg
//
LOCUS       DBNHQR010000646         5349 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_949650, whole genome shotgun sequence.
ACCESSION   DBNHQR010000646 DBNHQR010000000
VERSION     DBNHQR010000646.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5349)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5349)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5349
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_949650"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="AC16VR_14800"
     CDS             complement(<1..388)
                     /locus_tag="AC16VR_14800"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6012477.1"
                     /translation="MKLGILGGNIGRKTGINIEAIRKAESLGYDSVWTAEAWGGDAVT
                     PATWILAQTEKIKVGTAIMQMPARTPAMAAMTAMSLAELSGGRFIIGLGASGPQVVEG
                     WHGVPYGKPMTRLREYVAIMRKIFARE"
     gene            complement(423..2099)
                     /locus_tag="AC16VR_14805"
     CDS             complement(423..2099)
                     /locus_tag="AC16VR_14805"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR6012478.1"
                     /translation="MLSRSEALEALTAPGQPYELVDVELYGRSCRAFRNAPPLLRDLY
                     ASTASDLPFIVYGGERLSFADAWAESCRIAILLMEQYGVARGDRVAISMRNYPEWMTA
                     FAAVTSIGAIAVAMNALWTPEEMEYGLKDSGAKVLLADEERLARLALCADPPAGLAVL
                     AVRAADDAGTGARDLREALAGVPAGNMPAVDVAPEDDATILYTSGSTGHPKGAVSCHR
                     SLVTALLSWELDAHAGAYMQGVDLPAPESQPATLLAVPLFHATGSHAVYLASYRSQRR
                     VVSMYKWDVEEAAELIERERISSFVAPPAMTGDLVQFAARTNRDLSCLDRVGGGGAAR
                     APEQVRGIRRTFAKAMPSTGWGMTETNAIGTVISGQEYLDRPESSGRCSAVLELRVVD
                     PEGRELPCGERGELQVRGTSLIRGYWNRPEATAETIVEGWLRTGDVAYLDDDGYLFIV
                     DRIKDLVIRGGENIGCGAVEAALQEHPDVIEACVYAVPDERLGEEVGATVYICRPVDE
                     EEIRNFLAPRLARFEIPRYLSFSTQPLPRTASGKIFKRQLKQEAADRLTG"
     gene            2237..3025
                     /locus_tag="AC16VR_14810"
     CDS             2237..3025
                     /locus_tag="AC16VR_14810"
                     /inference="COORDINATES: protein motif:HMM:NF023358.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3450 domain-containing protein"
                     /protein_id="HIR6012479.1"
                     /translation="MKPYGKRHASWVWLTLFLLTPNPSRSEAPTATAGDALDQAQSYQ
                     TIAGRSQARVEDLDDAALAMLSQYNGEVDRYEDLRTYNDNLRELLASQRAEKERLNAE
                     LAGIEIVRQEIVPLMVQMIDVLDSFVALDQPLLIDERTSRVESLRRNLTRSDVDLAER
                     YRRIIEAYQIEAEYGQTIEAYEGPIVIDDRELTVDFLRVGRIGLYYLSLDRAEGGIWN
                     PAEKRWTPLAENYLDDLDLAVRMARKQAPPNLMDLPMWTTGGES"
     gene            3022..4374
                     /locus_tag="AC16VR_14815"
     CDS             3022..4374
                     /locus_tag="AC16VR_14815"
                     /inference="COORDINATES: protein motif:HMM:NF013760.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR6012480.1"
                     /translation="MRRTAITLLALLAPASWAAAPTTLDELIDSLKDSSALQAKRLQE
                     REARFVAARNERERLLEDARDRRREAEREADRLRADFEGSEQELADLETRLDENAGDL
                     RELFTVVRQITGDVAPMVESSLVSAEQGPRNALLRRLAEPGAIPAAEELRELWMILLE
                     EMNHSGRVSRFEAPVISADGAERTRRVTRIGTFTALADGSYLRYLPETGRLLALGRQP
                     AGVDVGDVLAFEEAREPLQRVAIDPSRGSILSLIVQTPDVRERIEQGGVIGYIILALG
                     ALGLLLGVERLVTIGVARQRTRRVLADSAAAGDHVIVELRELARDEAYLADAEALSAK
                     MDEVVTIASEKLRRGLPTLAIFAAVSPLLGLLGTVTGMIETFQVITLFGAGDPRLMSG
                     GISQALITTQLGLAVAIPLLLIQSFVQGRANALIATLDETAAELFAQGRTHRGSDHAG
                     "
     gene            4364..4903
                     /locus_tag="AC16VR_14820"
     CDS             4364..4903
                     /locus_tag="AC16VR_14820"
                     /inference="COORDINATES: protein motif:HMM:NF013760.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR6012481.1"
                     /translation="MLGDLFGNSDLIMAGGWIIVLIMALTFVMWMMILDRYVYLWRDR
                     GRAVSRKFALWQQYSSPDPLNNKRLRAALTGQFLEDSRRWIGTIQVITAVLPLLGLLG
                     TVTGMIKTFDVLTAFGTGNSRGMAEGISQALITTMAGLIAALTGIYFANDLEERIATE
                     ADHLAGRFNEGFADRGNGR"
     gene            4900..5310
                     /locus_tag="AC16VR_14825"
     CDS             4900..5310
                     /locus_tag="AC16VR_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR6012482.1"
                     /translation="MKLHTREEGGSAAINLTPLIDMVFILLIFFAVNATFVKITGVSV
                     DQPTAKTTSLQPASTILIAVTDAGEIWIDKKKVDKRRVRGVVERMFIEAPDAAVVVLS
                     DEESRTGLVVEVIDQVRLAGAGRVALAAAEPEEQ"
BASE COUNT          937 a         1770 c         1776 g          866 t
ORIGIN      
        1 cctcacgggc gaaaatcttg cgcatgatgg ccacgtactc tctcagccgc gtcatgggct
       61 tgccgtacgg cacgccgtgc cagccttcca ctacctgggg ccccgaagca ccgaggccga
      121 tgatgaaccg gccaccggag agctccgcca ggctcatggc cgtcatggcg gccatggccg
      181 gcgtccgagc aggcatctgc atgatggcgg tgcccacctt gatcttctcg gtctgcgcga
      241 gaatccaggt ggcgggcgtg accgcgtcgc ctccccaggc ttcggccgtc cagaccgagt
      301 cgtagccaag ggactcggcc ttgcgaatcg cctcgatgtt gatgcccgtc ttgcggccga
      361 tgttgccgcc gagaatgccg agtttcatgt gatatgcctc ctttgcgagt aatgcgatga
      421 gttcagccag tgagccggtc cgctgcctcc tgcttgagtt ggcgcttgaa gatcttgccc
      481 gatgcggtgc gcggcagcgg ctgggtggaa aaagacagat agcgggggat ctcgaagcgt
      541 gccaggcggg gagccagaaa gttcctgatc tcctcctcgt cgaccgggcg gcagatgtag
      601 acggtcgcgc cgacttcctc ccccaggcgc tcgtcgggca ccgcgtaaac gcacgcctct
      661 atcacgtccg ggtgttcctg caacgccgcc tccacggcgc cgcagccgat gttctcaccg
      721 ccccgtatga ccaggtcctt gatacggtcg acgatgaaga ggtaaccgtc gtcatccaga
      781 taggccacat ccccggtgcg aagccaccct tcgacgatcg tctccgcagt ggcctccggg
      841 cgattccagt aaccgcggat gagcgaggtg ccgcgaacct gcagctcgcc gcgctctccg
      901 cagggcagct cgcgaccctc gggatctacc acccgcaatt ccagcacggc gctgcagcgg
      961 cccgagcttt ccgggcgatc caggtattcc tgaccggaga tgacagtgcc gatggcgttg
     1021 gtctccgtca tgccccagcc cgtgctcggc atggccttgg caaaggtgcg tcggatcccg
     1081 cgcacctgct cgggcgcacg ggccgcaccg ccgccgccga cccgatccag gcagctcaga
     1141 tcccggttgg tgcgtgcggc aaactggacg agatcccccg tcatggccgg cggagcgacg
     1201 aaagacgaga tgcgctcgcg ctcgatgagc tcggcagcct cctcgacgtc ccacttgtac
     1261 atggaaacga cccgccgctg agaccggtag gaggcaagat agaccgcatg ggagcccgtc
     1321 gcgtgaaaca gcggcacggc gagcagcgtg gccggctgag actcgggagc cggcagatcg
     1381 acgccctgca tgtaggcgcc cgcgtgcgcg tcgagttccc aggaaaggag cgcggtgaca
     1441 aggctgcggt gacaggagac cgcgcctttg gggtgtcccg tggagccgga ggtatacaaa
     1501 atggtggcat cgtcctccgg ggcgacgtcc accgccggca tgttgcctgc cgggaccccg
     1561 gcgagggcct ctcgcagatc ccgggcgccg gtccccgcgt catcggcggc ccgcaccgcc
     1621 agaacggcta gacccgccgg cggatcggca caaagcgcca atcgggcaag gcgttcttcg
     1681 tctgccagga ggaccttggc gccgctgtcc ttcaggccgt actccatttc ctccggcgtc
     1741 cagagcgcgt tcatggccac cgcgatggcg ccgatggacg tgaccgcggc aaaggccgtc
     1801 atccactccg ggtagttgcg catggagatg gcgaccctat cgcccctggc gaccccgtac
     1861 tgttccatca gcaggatggc gattcggcag gactctgccc aggcgtccgc aaaggaaagg
     1921 cgctcgccgc catagacgat gaacggcagg tcgctggcgg tggacgcgta tagatcccga
     1981 agaaggggcg gcgcgttgcg aaaggcacgg cagctgcgac cgtacagctc gacgtccacg
     2041 agctcgtagg gttgacccgg cgcggtcagc gcctccaagg cctcggatct gctcaacatg
     2101 tgccttccac ctctcccgct gcaaacgata cccgcgctcg ctatgcgccg acaactcgcc
     2161 attacgggga ttcacggata accaaagcat tcgcgcccta tatcctcaaa atcgtgtaaa
     2221 cgtgggaggg gtcctcgtga agccatacgg caaacgacac gcgagctggg tctggctgac
     2281 cctttttctg ctgacgccga atccgtcgcg gagcgaggcg ccgaccgcta ccgcaggcga
     2341 cgctctggac caggcgcagt cctatcagac cattgcaggc cgcagccagg cccgggtcga
     2401 ggatctcgac gacgcggccc tggccatgct ctcccagtac aacggcgagg tcgatcgcta
     2461 cgaggacctt cgcacgtaca acgacaacct gcgggagctg ctggccagcc agcgcgccga
     2521 gaaggaacga ctcaatgccg agcttgccgg gatagagatc gtccgtcagg agatcgtccc
     2581 cctaatggtg cagatgatcg acgtcctcga cagcttcgtc gcgctggacc agccgctcct
     2641 gatcgacgag cgcacgtccc gggtggagtc gctgcgaagg aacctcacgc gttccgacgt
     2701 agacctggcc gagcgctacc gccgtatcat cgaggcgtat cagatcgagg cggaatacgg
     2761 gcagaccatc gaggcgtacg aaggacccat cgtcatcgac gaccgggaac tgaccgtcga
     2821 ctttcttcgg gtcggccgca tcgggttgta ctacctttcc ctggaccggg ccgagggcgg
     2881 catctggaac ccagccgaga agcgctggac gcccctggcg gaaaattatc tggacgatct
     2941 cgacctcgcc gtccggatgg cacgcaagca ggctccgcca aacctgatgg accttcccat
     3001 gtggacgacg ggaggcgaat catgaggcgg acggcgatca cgctgctggc cctgctcgcg
     3061 cccgcgagct gggccgcagc ccccaccacc ctggacgagc tcatcgactc cctgaaggac
     3121 tcgtccgccc tgcaggccaa gcgactgcag gagcgggagg cacggttcgt ggcagcccgc
     3181 aacgagcgcg agcgcctgct ggaggatgcc cgcgaccgcc gccgcgaagc cgagcgggaa
     3241 gcagaccggc tgcgggctga tttcgagggc tcagagcagg agctggccga tctggaaacc
     3301 cgtctcgacg agaacgccgg cgatctccgg gagctgttca cggtggtgcg ccagatcacc
     3361 ggggacgtgg ctccaatggt cgaaagctca ctggtctcgg cagagcaggg tccgcgcaac
     3421 gctctgctga ggaggctggc ggagcccgga gccatccccg ccgccgaaga gctgcgcgag
     3481 ctctggatga tcctgctgga ggagatgaat cacagcggcc gggtgtcccg gttcgaagcg
     3541 cccgtgatct ccgccgacgg cgcggagcgc acgcgccgcg tcacgcgcat cggcactttc
     3601 accgccctgg ccgacggcag ctatctgcgc tacctgccgg agaccgggcg cctcctggcc
     3661 ctcggccgcc aaccggcggg ggtcgacgtg ggcgacgtgc tggcattcga ggaagcccgg
     3721 gaaccgctgc agagagttgc catcgaccct tcccggggct ccattctctc gctgatcgtg
     3781 cagacgcccg acgtgcgcga gcgcatcgag cagggcggcg tcatcggcta catcatactc
     3841 gcgctcggcg cgctgggcct gctgctgggc gtggagcgcc tggtgaccat cggggtcgcc
     3901 aggcagcgga cgcgaagagt gctcgcggat tccgctgcgg ccggcgacca cgtgatcgtg
     3961 gagctcagag agctggcaag ggacgaggcg tatctggccg acgccgaggc gctcagcgca
     4021 aagatggacg aggtggtcac cattgccagc gaaaagctgc gccgggggtt gcccaccctc
     4081 gcgatctttg cagccgtgag ccctcttctg gggctgctgg gcacggtgac cggcatgatc
     4141 gagacctttc aggtcatcac cctgttcggc gccggcgatc cgcgcctgat gtcgggcggc
     4201 atatcccagg ccctgatcac cactcagctg ggtctcgccg tcgcgatccc gctgctgctg
     4261 atccagagct tcgtccaggg gcgggcaaac gcgctgatcg ccaccctgga tgaaaccgcg
     4321 gccgagctct ttgcccaggg ccgcacgcat cggggcagcg accatgctgg gtgatctttt
     4381 cggcaacagc gacctgatca tggccggcgg ctggatcatc gtgctgatca tggcgctcac
     4441 gttcgtcatg tggatgatga tcctggatcg ctacgtgtac ctgtggcgcg accgcggacg
     4501 agccgtctcg cgcaaattcg cgctgtggca gcaatactcg agcccggatc ccctgaacaa
     4561 caagcgcctg cgagccgcgc tgaccgggca gtttctcgag gattcccgtc gctggatcgg
     4621 caccattcag gtcatcaccg ccgtgctgcc gctgctggga ctcctgggga cggtcaccgg
     4681 catgatcaag accttcgacg tgctcaccgc gttcggcacg ggcaacagtc gcggcatggc
     4741 cgagggcata tcccaggcgc tgatcaccac catggcgggg ctgatcgcgg cgctcaccgg
     4801 catctacttc gccaacgatc tggaagagcg catcgccacc gaagcggatc atctcgccgg
     4861 ccggttcaac gaggggtttg cagaccgggg gaacgggagg tgaagctgca cacgcgggaa
     4921 gaaggcggca gcgcggccat caacctgacg ccgctgatcg acatggtgtt catcctgctc
     4981 atctttttcg cggtgaacgc cacgttcgtg aagatcaccg gcgtgtccgt ggaccagccc
     5041 acggccaaga ccacgagcct gcagccggcg agcacgatcc tcatcgccgt caccgatgcc
     5101 ggcgagatct ggatcgacaa gaagaaagtg gacaagcggc gcgttcgcgg cgtggtggag
     5161 cgcatgttca tcgaggctcc ggacgcggcc gtggtggtgc tgtcggacga ggagtcacgc
     5221 acgggcctgg tcgtggaggt catcgaccag gtgcgcctgg ccggggcagg cagggtggcg
     5281 ctggcggctg cagagcccga ggagcagtga tggcgctgcg ccagggtttc atcttcctcg
     5341 gcgcgctgg
//
LOCUS       DBNHQR010000647         1566 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_126213, whole genome shotgun sequence.
ACCESSION   DBNHQR010000647 DBNHQR010000000
VERSION     DBNHQR010000647.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_126213"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..1285
                     /locus_tag="AC16VR_14830"
     CDS             203..1285
                     /locus_tag="AC16VR_14830"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF009395.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR6012483.1"
                     /translation="MKYLTDDLRITGMEEVVAPNELIREIPLSDEVSELVFSNRQTIS
                     RILRGEDPRVLVIVGPCSIHDPEAALDYADRLAAKAREHQEHLQVIMRVYFEKPRTTV
                     GWKGLINDPHLNDSFDINQGLRIARGLLRDIALRGLGTATEYLDPITPQYLGDLVSWG
                     AIGARTTESQVHRQLASGLSCPIGFKNSTDGSIQVAVDAIKSAAHPHIFLSVTKEGHS
                     AIFSTAGNEDCHVILRGGGGKPNYDNPSVHYAARLLEQAGLNNRVMIDMSHANSEKDH
                     KKQLKVCDDICRQLADGEQRIFGVMIESNIVEGSQKMTKPESLTYGQSITDACIGWED
                     TERCLESLARASAERMAQSALDQDAS"
     gene            complement(1282..>1566)
                     /locus_tag="AC16VR_14835"
     CDS             complement(1282..>1566)
                     /locus_tag="AC16VR_14835"
                     /inference="COORDINATES: protein motif:HMM:NF013918.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HIR6012484.1"
                     /translation="GSDLTERPYIIAGFLALLLLLPLAITSTNGWRRRLGRRWFKLHR
                     LVYAAMGLVLLHHLWLTKDGYAEPAFYLACATALALERLLAARVAAARAR"
BASE COUNT          308 a          520 c          494 g          244 t
ORIGIN      
        1 gcttctaccg gcgcggccgc actcgcggcc tgttcggcgc cctgggcagc gcgcgcagcc
       61 tcagccgacg ggccgcccgg tgcgcgcagg cgccatacgt cgatgcccat ctgattgagc
      121 agccgcacgg cacgggagtc tggggcactg gtcatgacgt tcattgtaca cttgctgccc
      181 gtttctcttg cgacacgttc cgatgaagta tctgaccgac gacctgcgca tcaccggaat
      241 ggaagaggtg gtggcgccaa acgagctcat ccgcgagatt ccgctgagcg acgaggtatc
      301 cgagctggtg ttttccaatc gtcagaccat cagccggatt ctgcgtgggg aggatccgag
      361 ggtgctggtg atcgtaggac cctgttcgat ccacgacccg gaggcagcgc tggactatgc
      421 agaccggctg gccgcgaagg cgcgcgagca tcaggaacac cttcaggtga tcatgcgcgt
      481 gtacttcgag aagccgcgca cgaccgtggg ctggaagggc ctcatcaacg acccgcacct
      541 gaacgattca ttcgacatca accagggcct gcgcattgcc cgaggcctgc tgcgcgacat
      601 cgccctgcga ggtctaggca ccgcaaccga gtatctcgat ccgatcaccc ctcagtatct
      661 cggcgacctg gtttcctggg gcgccatcgg cgcccgcacc actgagtccc aggtgcaccg
      721 tcagctcgcc tccgggctgt cgtgccccat cggcttcaag aacagtacgg acggctcgat
      781 ccaggtcgcg gtggacgcca tcaaaagcgc ggcccatccg cacatcttcc tctcggtcac
      841 caaggagggg cattcggcca tcttctccac ggcgggcaac gaggactgtc atgtcattct
      901 ccgcggcggc ggtggcaagc cgaactacga caatccctcc gtgcactacg cggcccgcct
      961 gctcgagcag gccggactca acaaccgcgt catgatcgac atgagccacg ccaacagcga
     1021 aaaggaccac aagaagcagc tcaaggtctg cgacgacatc tgccgccagc tcgccgacgg
     1081 cgagcagcgc atcttcggag tgatgatcga gagcaacatc gtcgaaggta gtcagaagat
     1141 gaccaagccg gagtcgctca cctacggaca gagcatcacc gatgcctgca tcggctggga
     1201 ggacacggag cgctgcctcg agtcgctggc ccgagcgagc gcagagcgga tggcgcaatc
     1261 cgcgctcgac caggatgcga gctagcgcgc cctcgccgcc gcgacccgcg ccgccagcag
     1321 ccgctccagg gccagcgccg tcgcacacgc caggtaaaat gccggctccg catagccatc
     1381 tttggtgagc cagaggtggt gcagaagcac caggcccatg gccgcataga cgagccggtg
     1441 cagcttgaac cagcgcctgc cgagacggcg acgccagccg ttggtcgagg tgatcgccaa
     1501 tggcaggagc aggagcaggg cgaggaaccc tgcgatgatg tacggtcgct cggtgagatc
     1561 cgagcc
//
LOCUS       DBNHQR010000648         3706 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_950332, whole genome shotgun sequence.
ACCESSION   DBNHQR010000648 DBNHQR010000000
VERSION     DBNHQR010000648.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3706)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_950332"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="AC16VR_14840"
     CDS             complement(<1..847)
                     /locus_tag="AC16VR_14840"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007351952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HIR6012485.1"
                     /translation="MPPSLVIVESPAKAKTINRYLGSDYIVRSSVGHIRDLPVGGKSV
                     DPKARAREAAKTRKLSPEKKAEYKQERARQQLVRRMGVDPDKGWAAEYEILPGKEKVV
                     DELTKLAKNAEAIYLATDLDREGEAIAWHLRETIGGDPARYRRVTFNEITRRAIQEAF
                     SEPSELDMNQVHAQQARRFLDRVVGFELSPLLWGKIARGLSAGRVQSVAVRLVVERER
                     EIRAFIPEEYWEAFADLKRGADGDVLRFQLIGDGGSNFRPDNGEDAHEALGRLKDREL
                     VVRSRE"
     gene            complement(912..1484)
                     /locus_tag="AC16VR_14845"
     CDS             complement(912..1484)
                     /locus_tag="AC16VR_14845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012486.1"
                     /translation="MTWLIIGLILLAAFGPVLWLVPSRKDRHLSALREQARLEGLVVE
                     LRRLPKLQPSAHERVTAGGRVKKPVQECAAYMQMLRRRLVMLPGWRVHRGTGDLEARG
                     GWYFDFELRARGDAFDATMSALDPLFGGLPADVVAVELTDRTLLAYWLERGAATRETV
                     SEMALQMRAAEQALLDLDARFQSLAENDDS"
     gene            1558..2022
                     /locus_tag="AC16VR_14850"
     CDS             1558..2022
                     /locus_tag="AC16VR_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6012487.1"
                     /translation="MADYRRIMLAVDLTEESNHVAERAIAIAKAFDAELHLVHVIEPL
                     SLAYGGDIPMDLSSVQEQIHDQAKSHLAEFANRLGVPAERQNLIFGRPESEIHRVAES
                     SEADLIVVGSHGRHGLALLLGSTANGVLHGATCDVLAVRVGSIAEKSPDSDS"
     gene            2105..>3706
                     /locus_tag="AC16VR_14855"
     CDS             2105..>3706
                     /locus_tag="AC16VR_14855"
                     /inference="COORDINATES: protein motif:HMM:NF026068.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HIR6012488.1"
                     /translation="MFFSLLFVAPVAPSFANDDEYRTPYQQRTAYRAALSHLRVGNTQ
                     AFRRIESDLKGYSLHPYLEYHELRARLSSASASEVLAFRERHADLPVTPLLYQRWLER
                     LGQGREWKAFLDHWEESGDPELRCYHSRALYGTGQKEAAFAGVADLWIVGRSQPEACD
                     PLFKVWIDNDNLTEGMVWQRLDLALQANKRQLARYLQRFFSGSRKPWAQSFYNVHVTP
                     DLIARTDRFETDNELSRAVIAHGLRRLAARDAEKASAAWQSYQRSHDFSPETVRELDQ
                     SIAIALAGEGSFPSQRPEKPSSEFVMEMAKAAVAQQNWSEAYYWIERLPSETLGSTRW
                     QYWSARAVGSTHLDSERARLTLQALAERRDYYGFLAAEHLGIETKLNHSALALNPIHT
                     LQLNRMPAVQRALELYAVGDLLNARREWNRVLPDLDQARQVQAAFLIQQSGWTSQAIR
                     MANAASLSDHLDLRFPLAYPEFFKRASHVTAIPESFLIAIARQESAFDPRARSHADAL
                     GLMQLLPSTAKRVARRTGLTAPSDDD"
BASE COUNT          657 a         1250 c         1118 g          681 t
ORIGIN      
        1 cctcacggct cctgaccacc agctcccggt ctttgagacg ccccagcgcc tcgtgggcat
       61 cctcgccgtt gtccggccgg aagttgctgc cgccatcgcc tatcagctgg aatctgagca
      121 catcgccgtc agccccgcgc ttgagatcgg cgaaagcctc ccagtactcc tcggggatga
      181 aggcccggat ttcccgctcc cgctccacga ccagccgcac ggccaccgac tgcacccgac
      241 cggctgacag gccccgggca atcttccccc acagcagcgg cgagagctcg aagccgacga
      301 cacggtccag gaatcgcctg gcctgctgag cgtgcacctg gttcatgtcg agctctgacg
      361 gctcggagaa ggcctcctga atggcccgcc gggttatttc gttgaacgtc acccgccgat
      421 aacgagccgg atcgccgccg atggtctctc tgagatgcca ggcgatggcc tccccttcgc
      481 gatccaggtc ggtggcgagg tagatggcct cggcgttctt ggcgagcttg gtgagctcgt
      541 caacgacctt ttcctttccg ggcaggatct cgtattcggc cgcccagccc ttgtccgggt
      601 ctacgcccat gcgtctgacc agttgctggc gggcccgctc ctgtttgtac tccgcttttt
      661 tctccggaga cagcttgcgc gtcttggcag cctccctggc tcgcgccttg ggatcgacgc
      721 tcttgccgcc caccggcaga tcccgaatgt gacccacgct ggagcgcacg atgtagtcgc
      781 tgccaaggta acgattgatc gtcttggcct tggccggtga ttcaacgatg actaaagaag
      841 ggggcatatg tgcctgaaca gcgcccgagg atgggttcgg gcgccgttat acggcctggc
      901 caaaacggct gtcaagaatc gtcgttctcc gccaggctct gaaaacgggc atcaagatcg
      961 agcagcgctt gctccgctgc tcgcatctgc aaggccatct cggacacggt ttcccgggtg
     1021 gcagcgcctc gttccaacca gtaggcgagc agcgttcggt cggtcaactc caccgcaacg
     1081 acgtcagcgg ggagcccccc gaaaagaggg tccagagcgg acatcgtcgc gtcgaatgca
     1141 tccccccgtg cccggagctc gaagtcgaaa taccagccgc cgcgggcttc caaatccccg
     1201 gtgccacgat ggacgcgcca gccgggcagc atcacgagtc ttcgccgcag catctgcatg
     1261 tacgccgcgc actcctgcac cggtttcttg acgcgtcccc cggccgtgac ccgctcatgg
     1321 gcggacggct gcagcttggg caatcgcctc aattccacca cgagcccctc gagtcgggcc
     1381 tgctctcgca acgcgctgag gtggcggtct tttctgctgg gcaccagcca gagcaccgga
     1441 ccgaaggcgg ctagcaggat caaaccgatg attaaccacg tcatgggacg ctccccgaga
     1501 ggttgagggg cctttggatt gtgtgtatcg tcgccgcaat tcgtgccggg aggcaacatg
     1561 gctgactacc gtcgaatcat gttggcagtg gatctcaccg aggagtccaa tcacgtcgcg
     1621 gagcgggcta tcgctattgc gaaggccttc gacgcggaac ttcatctcgt gcatgtcatc
     1681 gaaccattga gccttgccta cggtggcgac attcccatgg acctgtcgtc tgttcaggag
     1741 cagatccacg atcaggctaa atcccatctc gccgagttcg ccaaccgtct gggcgttccg
     1801 gcagagcgcc agaacctcat cttcggacgc cccgagagtg agatccatcg cgtcgccgaa
     1861 tccagcgaag ccgacctcat cgtggtggga agccacggcc ggcacggcct tgccctgctg
     1921 ctcggctcca ccgccaacgg cgtgctgcac ggcgccacct gcgacgttct ggccgttcgc
     1981 gtgggcagca tcgcggagaa gtcgccggat tccgatagct gatctgattg ccagtctaag
     2041 gggccggctg accctcccgt cacccagcac cgctctgccc ggccactgtc ggcgctggca
     2101 cctgatgttc ttctcgctgt tgttcgtggc gccggtggcg ccgtcgttcg ccaacgatga
     2161 cgagtaccgc accccatacc agcagcgcac cgcgtatcgg gccgctcttt cgcacctgcg
     2221 ggtcggcaat acgcaggcgt ttcgccgcat cgagtcggac ctgaagggtt actcgctaca
     2281 cccgtatctg gagtaccacg agctccgcgc tcgcctgtcc agcgcttcgg cgagcgaggt
     2341 gctggcattt cgcgagcggc acgccgacct tccggtcacc ccgcttctct accagcgctg
     2401 gctggagcga ctcggccagg ggcgagagtg gaaggcgttt ctcgaccact gggaggaaag
     2461 cggcgatccg gagctgcgtt gctatcacag ccgggccctt tacggcaccg gccagaagga
     2521 ggctgctttc gcaggcgttg cggacctgtg gatcgtaggc aggtcccagc cggaagcgtg
     2581 cgatccgttg ttcaaggtgt ggatcgacaa cgacaacttg acggagggca tggtctggca
     2641 gcgcctggat ctggcgctac aggccaacaa acgtcagctc gcccggtatc ttcagcgctt
     2701 cttctccggt tcacgaaagc cgtgggctca atcgttctac aacgtgcacg tcacgccaga
     2761 cctcatcgcg cggactgacc gcttcgagac ggacaacgag ctttccagag ccgtgatagc
     2821 ccacggattg cggcgcctgg ccgcccgaga cgcggagaag gccagcgcag cgtggcagag
     2881 ctaccaacgg tcccacgact tttccccgga gaccgtccgg gagctggacc aatcgattgc
     2941 gatagcgctt gccggcgaag gctcttttcc atcgcaacgg ccagagaagc catcgtcaga
     3001 attcgtgatg gaaatggcca aggcggcggt ggcccagcag aactggtccg aggcctacta
     3061 ctggatcgaa cgtctgccgt cggaaaccct gggaagcacc cgctggcaat actggtccgc
     3121 acgggccgtc gggtccactc accttgattc ggagcgcgcc aggctgaccc tgcaggcctt
     3181 ggcagaaaga cgcgattact acggatttct cgccgcggag cacctcggta tcgagacgaa
     3241 gctgaaccac tcggcgctgg cactgaaccc catccatacc ctgcagctga atcgcatgcc
     3301 agcggtgcaa agagccctgg agctgtatgc cgtcggcgat ctcctcaacg cccggcgcga
     3361 gtggaaccgc gtgctgcccg atctggatca ggcacgccag gtgcaggcag cctttctcat
     3421 tcagcaatcc ggctggacct cacaggccat ccgcatggcc aacgcggcaa gcctgtcgga
     3481 ccatctggat ctgcgcttcc cgctggccta tcccgagttt ttcaagcgcg caagccacgt
     3541 caccgccatt cccgaatcct ttctgatcgc aatcgctcgc caggaaagcg ccttcgatcc
     3601 gcgggcccga tctcacgccg acgcgttggg gctcatgcag ctgctgccca gcacggcaaa
     3661 gagggtggcg cggcgaaccg ggctgaccgc cccgtcggac gacgac
//
LOCUS       DBNHQR010000649         5219 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1519772, whole genome shotgun sequence.
ACCESSION   DBNHQR010000649 DBNHQR010000000
VERSION     DBNHQR010000649.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5219)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1519772"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..1033)
                     /locus_tag="AC16VR_14860"
     CDS             complement(35..1033)
                     /locus_tag="AC16VR_14860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIR6012489.1"
                     /translation="MINKKVVLASRPEGQPKESDFKVIEEELAELTEGQVLLATEHLS
                     IDAFIRTMLDSQEGLHGTVAIDTPVMALGVARVVDSRSPDLNQGDAVFGPMGAQTHLV
                     APGGMYRKLEEGDVPPRSYLGALGMTTGLTAYAGMLKVGQVAEGDTVVVSAAAGAVGT
                     IACQLAKIAGAKVIGIAGGPAKVAYLIDEIGCDGAIDYKNDDVAEKLRALAPAGVNVF
                     FDNVGGELLDIVLDQIAQGARVVICGAISQYNHMEEVRGPRLYLRIPERNASMLGFTV
                     DRFAADFPEMEADISRWMAEGKLKMPEHVVEGIEQFPKALTMLFTGGHMGKLLVAP"
     gene            complement(1074..2393)
                     /locus_tag="AC16VR_14865"
     CDS             complement(1074..2393)
                     /locus_tag="AC16VR_14865"
                     /EC_number="1.13.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF015043.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006720 - isoprenoid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HIR6012490.1"
                     /translation="MSEAVSNEAPFWLSGNFAPTFDEVTETRLEVTGTIPPELKGRYL
                     RNGANPESGVTPHWFLGNGMIHGVELNDGRANWYRNRYVRTPLLSDGDHSDGVDERAK
                     SLANTHIIGHAGRILALEEGHWPFELTPELDTVGPYTFGGRLETGMTAHPKICAETGE
                     LLFFAYAATPPFMTYHRATADGELVQSEVIEVKGATMVHDFNITRNYVIFMDLPVVWN
                     FENAASVGLPLAWSDEYGARLGVMPRNGSNADVVWYDIDPCYVYHPLNAYEDGDDIVI
                     DVCRMAHHMKIGAPEAPPMLHRWTIHQSAGTVSEAQLDERSVDFPRVPDALVGQKHRY
                     GYTAEFGGGLPAAVAFRKYDLATGTSEAHVLADGRAGGEPVFVPARGGTSEDDGYLMS
                     FVYDPADNRSELVIIDASNVTADPVARVHLPVRVPAGFHGSWVADAQ"
     gene            complement(2390..3040)
                     /locus_tag="AC16VR_14870"
     CDS             complement(2390..3040)
                     /locus_tag="AC16VR_14870"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6012491.1"
                     /translation="MVAHSKSVTYTRAPAEKRERLLAAARELFAERGFEDTSTQQIAK
                     TAGVSEGILFHHFGSKRGLLERIAEDFVAAGVQAAMPDTPGQLSEEMVVRGAFDFADA
                     HPALYRLLEQMSRELGGSSGASRSAVIVAAIEKSLRAGMAQGIVREGDAGIMAELQFA
                     LVDAAYKAWRDGGDPDRREDYIREAVGCMKAMLAPSGAEERPPLADATKLSRGDAT"
     gene            complement(3107..4441)
                     /locus_tag="AC16VR_14875"
     CDS             complement(3107..4441)
                     /locus_tag="AC16VR_14875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HIR6012492.1"
                     /translation="MSNQRAAEFWARTRDVAGTWGLALALLLAGLIVAYQFVGPPPPK
                     RFVLATGESSGAYYYFGERFAQVLARDGVVVELRATGGSVDNLDLVAREGGADLAFVQ
                     GGIASSAPVDNLVTMGSLYLEPLWLFLGPGVVLDDLGDLKGRRLAVGVEGSGTRAVVM
                     QLLEANGVDAANSKLLGVASAALPDALAGGEIDAAFLIGAPQSSTVGKLLRLPGVRLH
                     DMERVEAYTRRNAFLSSVVLPRGVLDLEADLPAKDTETVAVGAMLVARDDFHPALTDL
                     LLIAAAEIHGGHSILADAGTFPTPRFVDLPLSEEAERHYRHGAPFLMRYLPFWAATLV
                     DRLWIMLVPLIGLSLPLARLVPPAYRWQIRRRLLRIYTKLHALDPLRTPIADDEDVAR
                     RLEELDRLDVDSTIEAVPKGYADDLYQLRRDIDLVRRRLGAARSAGRGQQND"
     gene            complement(4452..4826)
                     /locus_tag="AC16VR_14880"
     CDS             complement(4452..4826)
                     /locus_tag="AC16VR_14880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6012493.1"
                     /translation="MTSSIQTGAVLVVFGLAIGAASASAEVCDVDQVLQRLQSEVLGA
                     LSEEQQTRAREVLGEFCGGMISESPRRVVSTADPTEEAASKAPGELQEAEESEGTLKV
                     LGVEFQKADEDSRGHERLKKKR"
     gene            4945..>5219
                     /locus_tag="AC16VR_14885"
     CDS             4945..>5219
                     /locus_tag="AC16VR_14885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6012494.1"
                     /translation="MYDHLIRNATIVDGLGGKPCTGDIAIKDGVIAAVGEVSGNARET
                     LNAEGAYATPGWVDVHTHYDGQVSWDDTIEPSFSQGVTSIVMGNCGVG"
BASE COUNT          903 a         1789 c         1615 g          912 t
ORIGIN      
        1 aaaagccgcc gcgcgcgtgc ggcgtcgtgc cggatcacgg agcgaccagg agcttaccca
       61 tgtggccgcc cgtgaacagc atggtcagcg ctttggggaa ctgctcgatg ccctcgacga
      121 cgtgctcggg catcttgagc tttccttcgg ccatccagcg cgagatgtcg gcctccatct
      181 ccggaaagtc cgccgcaaag cggtccacgg tgaagcccag catgctggca ttgcgctcgg
      241 gtatcctgag ataaaggcgc ggccccctga cctcttccat gtggttgtac tgagagatgg
      301 cgccgcagat caccaccctg gctccctggg caatctgatc gagcacgatg tcgagcagct
      361 cgccgcccac gttgtcgaag aacacgttca cgccggcagg cgcaagggct cttagcttct
      421 ctgccacgtc gtcgttcttg tagtcgatgg caccatcgca accgatctcg tcgatcaggt
      481 aggcaacctt ggccggcccg ccggcgatgc cgatgacttt ggcgccggct atcttggcca
      541 gctggcacgc gatggttccc accgcacccg cggcggcgga aaccaccacg gtgtcgcctt
      601 cggccacctg tcccaccttg agcatgccgg cataggccgt aaggcccgtg gtcatcccca
      661 gcgcgccgag atagctgcgc ggcggcacgt cgccctcctc gagcttccgg tacatgccgc
      721 ccggcgcgac gaggtgagtc tgtgccccca tagggccgaa cacggcatca ccctggttca
      781 ggtcgggcga gcggctgtcc acgacgcgcg ccacgcccag ggccatcacg ggcgtatcga
      841 tggccaccgt gccatggagc ccttcctggg agtccagcat cgttctgatg aaggcgtcga
      901 ttgagagatg ctccgtcgcc agcagcacct gcccttcggt cagctccgcc agctcctcct
      961 cgatgacctt gaaatccgac tccttcggct ggccttccgg gcgtgatgcc agcactacct
     1021 tcttattgat caacgtcgtc tcctcgtcac gttgttgttc gccattaagg cggctactgg
     1081 gcgtccgcga cccagctgcc gtggaatccg gccggcaccc gcacgggcag gtgcaccctc
     1141 gcgacggggt cggcggtgac gttgctggca tcgatgatca ccagctcgct gcggttgtcc
     1201 gccgggtcgt agacgaagct catgagatat ccatcgtctt cggacgtacc ccctcgtgcc
     1261 ggaacgaaga ccggctcgcc gcccgcacgg ccgtcggcca gcacgtgcgc ctcggatgtt
     1321 cccgtggcca ggtcgtactt acggaaggcc accgcggcgg gaagcccacc accgaactcc
     1381 gccgtgtagc cgtagcgatg cttctgaccg accagcgcat cgggcacgcg cgggaaatct
     1441 accgaccgct cgtccagctg cgcttcgctg acggtgcccg ccgactgatg aatggtccag
     1501 cgatgcagca tcggcggcgc ttccggcgcc ccgatcttca tgtggtgggc catgcgacag
     1561 acgtcaatga cgatgtcgtc gccgtcctca taggcattca gcgggtggta gacgtagcag
     1621 ggatcgatgt cgtaccacac cacgtcggcg ttggagccgt tgcggggcat cacgccgagg
     1681 cgcgcgccgt actcgtcgct ccaggccagc ggaagaccga cagaggcggc gttctcgaaa
     1741 ttccacacca cgggcaggtc catgaagatc acgtagttcc gcgtgatgtt gaagtcgtgg
     1801 accatggtcg cgcccttgac ctcgatgacc tcgctctgaa cgagctcgcc atcggcggtc
     1861 gcgcggtgat aggtcatgaa ggggggcgtc gccgcgtaag caaagaagag cagctcgccg
     1921 gtctccgcgc agatcttcgg atgggccgtc atgccggtct ccagccggcc gccgaaagta
     1981 taaggaccaa cggtgtcgag ctccggggtc agctcgaagg gccagtgtcc ttcttccagc
     2041 gccagaatcc ggcccgcgtg gccaatgatg tgggtgttgg caagggactt ggcgcgctcg
     2101 tccaccccat cgctgtggtc cccgtcgctg agcaggggcg tgcggacata gcggttgcgg
     2161 taccagttcg cccgaccgtc gttcagctcg acgccgtgaa tcatgccgtt gcccagaaac
     2221 cagtgcggcg tgacgccgga ttccgggttg gcgccgttgc gcaggtaacg tcccttgagc
     2281 tccgggggga tggtcccggt cacctcgagc ctggtctcgg tcacctcgtc aaaggtcggg
     2341 gcgaaattgc ccgagagcca aaagggtgct tcgttgctga ctgcttcgct catgttgcgt
     2401 ctcctcgact gagtttcgtt gcgtccgcca gcggcggtcg ttcctcggct ccgctcgggg
     2461 ccagcatggc tttcatgcag ccgaccgctt cgcggatgta atcttctctg cggtccggat
     2521 caccgccgtc ccgccatgcc ttgtaggccg cgtccaccag ggcgaactgc agctcggcca
     2581 tgattccggc atccccttcc cggacgatgc cctgagccat tccagcccgc aggctcttct
     2641 cgatggccgc gacgatgaca gcgctgcggc tggcgccact cgacccgccg agttctcggc
     2701 tcatctgctc gagcagccgg tacagtgccg gatgggcatc ggcaaaatcg aacgcccccc
     2761 gcaccaccat ctcctcgctg agctggcccg gcgtatcggg catggccgcc tgaacgccgg
     2821 cggccacgaa atcctctgcg atgcgctcga gcagccctcg tttcgaaccg aagtggtgaa
     2881 agaggatgcc ttccgagacc cccgccgtct tggcgatctg ctgagtcgaa gtgtcttcga
     2941 acccgcgctc ggcaaacagc tcccgggcgg ctgccagcaa tcgctcccgc ttttccgcag
     3001 gggccctggt gtaggtaacc gatttagagt gcgcgaccat ttattgagta gcactttaca
     3061 aagtgttact caacaagtaa acccccaatt tgcggcatgc aggttttcag tcgttctgct
     3121 ggccgcggcc cgccgagcga gccgccccaa ggcgccgccg gacgaggtcg atgtcgcggc
     3181 gaagctggta gaggtcgtcg gcatagccct tcggaaccgc ctcgatagtc gagtccacgt
     3241 ccagccgatc gagctcctcc agccgacggg ccacgtcctc atcgtcggcg atgggcgttc
     3301 gaagcggatc cagggcgtgc agcttcgtgt agatgcgcag cagccgccga cggatctgcc
     3361 atcgatacgc agggggcacc agccgcgcca gcggcaggct caacccgatc agcgggacga
     3421 gcatgatcca gagccggtca accagcgtgg ccgcccagaa cggcaggtag cgcatgagaa
     3481 acggcgcccc gtgccggtag tgcctctccg cctcctcgct gagcggcagg tcgacgaaac
     3541 gcggcgtggg aaacgtgccc gcatccgcga gaatgctgtg accaccgtgt atctcggcgg
     3601 ccgcgatgag cagaaggtcc gtgagcgccg gatggaaatc atcacgggcc acgagcatgg
     3661 cccccacggc aaccgtctcg gtgtccttcg ccggcaggtc cgcttccaga tccagaacgc
     3721 ccctgggcag gacgactgaa gagaggaagg cgttgcgccg cgtgtaagcc tcgacccgtt
     3781 ccatgtcgtg cagacgaact cccggcaggc ggagaagctt cccgaccgtg gaggattgcg
     3841 gcgcgccgat gagaaacgcc gcgtcgatct cgcccccggc cagcgcgtcg ggaagcgcgg
     3901 ctgacgccac ccccagaagc ttcgagttcg ccgcgtccac cccgttggcc tcgagcagct
     3961 gcatcacgac ggcgcgtgtt ccgctgcctt ccacacccac cgccaaccgc cggcccttca
     4021 gatcgcccag gtcgtcgagc accacgcccg gtccaagaaa cagccagagg ggttccagat
     4081 acaggctgcc catagtgaca aggttgtcca cgggggcaga cgacgcgatg cccccctgca
     4141 cgaaagcgag gtcggccccc ccttccctgg ccaccagatc gagattgtcc acgctgccac
     4201 cggtggcgcg cagttcgacc accaccccgt cccgggccag aacctgggca aaccgctcac
     4261 cgaaatagta gtaagcgccg gacgactccc cggttgcgag aacgaaccgt ttcggcggcg
     4321 gcggcccgac gaactgatag gcgacgatga gccccgccag caggagcgcg agcgcgagcc
     4381 cccaggttcc cgctacgtcc ctggtccggg cccagaattc agctgcacgt tgattggaca
     4441 tgctcgaacg gtcaacgctt cttcttcagg cgctcatggc cgcgcgaatc ctcgtcggcc
     4501 ttctgaaatt ccaccccgag caccttcaac gtcccctccg actcctcggc ttcctgcaac
     4561 tccccgggcg ctttggaggc agcctcttcg gtcgggtcgg ccgtgctgac cacccgtctg
     4621 gggctttccg aaatcatgcc gccgcagaat tccccaagca cctcgcgggc tcgcgtctgc
     4681 tgttcttccg acagcgcgcc cagcacttcg gattgcaggc gctgtagtac ctgatcgaca
     4741 tcgcaaacct ctgcgcttgc cgaagccgcg ccgatcgcga gcccgaagac gacgagaacg
     4801 gccccggttt gaatcgagct ggtcatggcg gcattatgca ccaccgcggc tcagacgtgc
     4861 tgccgttcgt gcacgagagg ccaccctttt cttcgttacg cccacagtca ccacgacggg
     4921 cacggtattg ctcgaggagc caccatgtac gaccacctga tcaggaacgc caccatcgtc
     4981 gacggtctcg gcggaaagcc atgcacgggc gacattgcca tcaaggatgg cgtcatcgcc
     5041 gcggtgggcg aagtctccgg aaacgccagg gagaccctga acgcggaggg cgcctacgcc
     5101 actcccggct gggtggacgt gcatacccac tacgacggtc aggtcagctg ggacgatacc
     5161 atcgagccgt cgttcagcca gggtgtgacc agcatcgtca tgggcaactg cggcgtcgg
//
LOCUS       DBNHQR010000650         3126 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1710217, whole genome shotgun sequence.
ACCESSION   DBNHQR010000650 DBNHQR010000000
VERSION     DBNHQR010000650.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1710217"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="AC16VR_14890"
     CDS             complement(<1..555)
                     /locus_tag="AC16VR_14890"
                     /inference="COORDINATES: protein motif:HMM:NF013225.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HIR6012495.1"
                     /translation="MQDIIEELQARRAAADLGGGEHRIEAQHGKGKLTARERISLLLD
                     EGSFEEWDKFVEHRCTDFSMEEQKIPGDGVVTGYGTVNGRLVFVFSQDFTVFGGALSE
                     AHAEKICKVMDQAMKVGAPLIGLNDSGGARIQEGVASLGGYAEIFQRNVLASGVVPQI
                     SLIMGPCAGGAVYSPAMTDFIFMVK"
     gene            792..1982
                     /locus_tag="AC16VR_14895"
     CDS             792..1982
                     /locus_tag="AC16VR_14895"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6012496.1"
                     /translation="MHHYLLGTASWFTAFGIQGVMFAWLVTMVLRESPEMVGLAQMTL
                     LLPGTLLMLIGGTYADRFGGRRIVIGAQSVAACAPLLLLVLIWSDMLTYASMLVYAVF
                     MGCATAFVTPARDGLLNEIAEGRVQRAVMLASMIQFGGQMIGFTLAAGADLVGPLVVL
                     GAQSLCLALGVLAFVLIPRPHEGRHVRTGRLLASLAEGARTVFSSASMRLIVVQNVAM
                     AIFFMGSYVVAMPLLVREVFNGTARDLAFTNGSNSLGLVLSIVLMLRLGDVTRQGRAL
                     VLSQLAGGLMLIAAALMTSFALYVLMLFIWGACGGIAMTMARTIMQEQAPDAQRSRVM
                     SFYALSFMGAGPLGALFTGYMVELLGPQQALVLSGSLMAVVACILAVGSNLWSLRAYQ
                     VASA"
     gene            1984..>3126
                     /locus_tag="AC16VR_14900"
     CDS             1984..>3126
                     /locus_tag="AC16VR_14900"
                     /inference="COORDINATES: protein motif:HMM:NF017215.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6012497.1"
                     /translation="MSRRDATYRLAVDIGGTFTDVVYEPDAAGAQRFTVKVLTTYDDP
                     ANGVMQGIEAVLERAGAEAGDAGLVLHGTTLATNALIERRGARTALLTTEGHRDALEM
                     ALENRFEQYDVNIDRPRPLVPRRLRLPVRERLDAAGRVLVPMDSKSLDAAVAALDGHG
                     VESVAIGFLHAYANPDHEHAAAERVREKLPDVSITLASQVCPEIREYERLSTACANAY
                     VKPLMARYLGSLQRKLTDRGMVCPVLLMTSGGGLTTLETAAEFPIRLVESGPAGGALL
                     AARIAAEGGHERVLSFDMGGTTAKICLIDGAAPLLSRSFEVDRTYRFKKGSGLPVRIP
                     VIEMVEIGAGGGSIAGVDKLRRIQVGPESAGSEPGPACYGLGGERAT"
BASE COUNT          451 a          998 c         1071 g          606 t
ORIGIN      
        1 cttgaccatg aagatgaagt cggtcatggc cggagagtag acggcgccgc cggcgcaggg
       61 gcccatgatc agcgagatct gcggcaccac gcccgaggcc agcacgttgc gctgaaagat
      121 ctcggcgtag ccgccaagag acgcaacccc ttcctggatc cttgcgccgc cggaatcgtt
      181 caggccaatc agaggcgcgc cgaccttcat ggcctggtcc atcaccttgc agatcttctc
      241 ggcatgggct tcggacaggg cgccgccgaa cactgtgaag tcctggctga agacgaacac
      301 cagccgcccg ttcaccgtgc cgtagccggt gacgacgccg tctccgggga tcttctgctc
      361 ctccatggag aagtcggtgc agcggtgctc cacgaacttg tcccattcct cgaagctgcc
      421 ctcgtcgagc aggagggaaa tgcgttcgcg ggctgtgagc ttgcccttgc cgtgctgtgc
      481 ttcgatgcgg tgctcgccgc cgccgagatc ggctgccgcg cgtcgcgcct gcagctcctc
      541 gatgatgtcc tgcatctggc tctcctgccg tgggatgccc ccccggaccg ggtgcggatg
      601 ctcccggagg gatatggttg ttgttgtccg agacgaaacc gcgaggatat cctgccgcgt
      661 tcgcggtgac cacatcttgc gcgcgccgtg ccgggcgctt caggggcggt ccggccagcc
      721 cctcgaggcg gtatactgcg gcgtcgtttc ctgctcggag cggttttcgc cgcaacgata
      781 tcttgaagcc catgcatcat tacctgctcg ggaccgcgag ctggtttacc gcgttcggta
      841 tccagggcgt catgttcgcg tggctggtca cgatggtgct gcgcgaatct ccggagatgg
      901 ttggtctggc tcagatgacc ctgctgctcc cagggaccct cctgatgctg atcgggggca
      961 cttatgcgga ccgcttcgga ggccggcgca tcgtcatcgg cgcgcaatcc gtggcggcct
     1021 gcgcgccgct gcttctgctc gtcctgatct ggagcgatat gctcacttac gccagcatgc
     1081 tggtctacgc ggtgttcatg ggttgcgcca ccgctttcgt caccccggcc cgcgacggtc
     1141 tgctcaacga aatcgcggag ggcagggtcc agcgcgcggt catgctggcc agcatgatcc
     1201 agttcggcgg gcagatgatc ggcttcacgc tcgctgcggg cgccgacctg gtgggtcccc
     1261 tggtggtgct cggcgctcag agcctctgcc tggcgctcgg cgtgctggca ttcgtgctga
     1321 ttccgcgtcc ccacgagggg cggcacgtgc gcacgggccg gttgttggct tccctggccg
     1381 agggtgcccg gaccgtcttc tcgtctgcgt cgatgcggtt gatcgtggtg cagaacgtgg
     1441 cgatggccat cttcttcatg gggtcttacg tggtggccat gccgttgctg gtccgggagg
     1501 tgttcaacgg caccgcccgg gacctggcct ttaccaacgg ctcgaactct ctcgggctgg
     1561 tgctcagcat cgtcctcatg ctccggcttg gcgatgtgac ccgtcagggc cgggcgctgg
     1621 tgctgtcgca gctggcaggt ggcctcatgc tgatcgccgc ggcgctgatg acgagctttg
     1681 ctctttacgt gctcatgttg ttcatctggg gcgcctgtgg cggaatagcg atgaccatgg
     1741 caagaaccat catgcaggag caggcgcctg acgcccagcg cagccgggtg atgagctttt
     1801 acgcgctctc gttcatgggg gccgggcccc tgggagcgct cttcacgggc tacatggtgg
     1861 agctgctcgg cccccagcag gcgctcgtcc tgtcgggcag cctgatggcc gtggtggcct
     1921 gcattctcgc cgtgggctca aatctctggt ctctgagagc ttatcaggtg gcgtctgcct
     1981 gacatgagca gaagggacgc aacgtaccgg ttggccgtgg atatcggtgg tacgttcacc
     2041 gacgtggttt acgagcccga tgcggcgggt gcgcagcgct tcaccgtcaa ggtgctgacg
     2101 acctacgacg atcctgccaa cggcgtgatg caggggattg aagccgtgct ggaacgcgcc
     2161 ggcgccgagg cgggcgacgc ggggctcgtt cttcatggca ccacgctggc gaccaacgcg
     2221 ctgatcgagc gccgaggcgc gcgaacggcg cttctgacca ccgagggtca tcgcgatgcc
     2281 ctggagatgg cgctggagaa tcgtttcgag cagtacgacg tgaacatcga ccggccgcgg
     2341 cccctggttc cccggcgctt gcgcctgccc gtccgggaac gtctggacgc cgctggcagg
     2401 gtgctggtac cgatggattc aaagtctctg gacgctgcgg tggccgccct ggacgggcac
     2461 ggggtcgaga gcgtggcgat cggtttcctg cacgcctacg cgaaccctga tcacgagcac
     2521 gccgcggccg agcgcgttcg cgagaagctg cccgacgtct cgatcacgct ggcctcccag
     2581 gtctgtcccg agatccgcga gtacgagagg ttgtcgacgg cgtgcgccaa cgcctacgtc
     2641 aagccgctca tggcccgcta tctgggcagt ctgcagcgta agctcactga tcgcgggatg
     2701 gtctgccccg tcctcctgat gacgtcgggc ggtgggctca ccaccctgga gacggcggcg
     2761 gagtttccca tccgcctggt ggagtcggga cccgcaggcg gggcgctgct cgccgcccga
     2821 atcgcggctg agggtggcca tgaacgggtg ctgtccttcg atatgggcgg aactacggcc
     2881 aagatctgtc tgatcgacgg cgccgcgccg ctgctttccc ggtccttcga agtggatcgg
     2941 acttaccgct tcaagaaggg aagcgggctg ccggttcgaa ttcccgtcat cgagatggtc
     3001 gagatcggcg ccggcggcgg atcgatcgcc ggagtcgaca agctgcgccg catccaggtg
     3061 gggcccgaaa gcgccggctc ggaacccggg ccggcgtgct acgggctcgg cggcgaacgc
     3121 gccacc
//
LOCUS       DBNHQR010000651         2664 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04982
            SRR25158346_k127_1203581, whole genome shotgun sequence.
ACCESSION   DBNHQR010000651 DBNHQR010000000
VERSION     DBNHQR010000651.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414390
            Sequence Read Archive: SRR25158346
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:09:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,984
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158346_k127_1203581"
                     /isolate="MAG04982"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.10 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="AC16VR_14905"
     CDS             <1..282
                     /locus_tag="AC16VR_14905"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6012498.1"
                     /translation="TQSLAAELAPEGIRVNALCPGMVGTAMWLEHLLPANAAKPDEKD
                     DQFKEAMARTIPMGRPQTPQDMGHAAVYLATAANVTGVSLSVAGGYEMN"
     gene            279..1088
                     /locus_tag="AC16VR_14910"
     CDS             279..1088
                     /locus_tag="AC16VR_14910"
                     /inference="COORDINATES: protein motif:HMM:NF024545.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR6012499.1"
                     /translation="MKKPTAVQNLFREPLLYFLLAGAAIFALAELTDGPGDDYLIEVS
                     DAERARISDQWQAQMGRAPTDEELAGLIEQWLREEIYYREALRMGLDRNDIIIRRRLA
                     QKLTFLTEDVIAGSTPSTEELRSYFEANAERYRIPERITFRHRYFSAERRPNAREDAA
                     AALTDEAVRGDPFMLQKAYALRSSREIADLFGVEFAEGLAQVAPGNWQGPIRSAYGWH
                     VVLVEQRAPGRLPSLEEVQKRAATDLSQERRSRANDEYYHSLRQRYEIIGG"
     gene            1085..2050
                     /locus_tag="AC16VR_14915"
     CDS             1085..2050
                     /locus_tag="AC16VR_14915"
                     /inference="COORDINATES: protein motif:HMM:NF025167.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HIR6012500.1"
                     /translation="MKALAALLVVLAASQAKAHEVRPAYLQLTETAPGQFEVLWKQPL
                     LGDRRLPLEPVLPEACAREGPPIPEVAGAALIERWRTDCDLRSGAVTISGLSKTLTDV
                     LVEIRYLSAEPVSQLLKPSRPSLDLASPTPQIWSYLTFGVEHLLLGLDHILFVVGLVL
                     FIGNTWTLVKTITGFTIAHSITLACSVLGLISLPQAPVEAVIALSILFLARELVEPPE
                     RRSRITLGRPWLMAFAFGLLHGFGFAGALTEIGLPADQLAVSLLLFNLGIEIGQLLVI
                     GLLLSAAAIFRKAWRRNREVWPRRFAWVMGSVAGFWTIDRVLLVF"
     gene            complement(2083..2490)
                     /locus_tag="AC16VR_14920"
     CDS             complement(2083..2490)
                     /locus_tag="AC16VR_14920"
                     /inference="COORDINATES: protein motif:HMM:NF016060.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HIR6012501.1"
                     /translation="MTDFRTQSRRQVFRYLQVGFAGFVTDVAVLSALVYGMSLGETET
                     GLVGSRFVSFLAAVTVTFLLNARYTFGASIRDSRFPVYVLVQSLGAAITLGTYTALVL
                     HTTLSQAPVVALIIGSAAATTSNYVLVRRFVYV"
BASE COUNT          476 a          895 c          851 g          442 t
ORIGIN      
        1 acccagtccc tggcggccga gctcgcgccc gagggcatcc gcgtcaacgc cctgtgtccc
       61 gggatggtgg ggaccgccat gtggctcgag cacctgcttc ctgccaacgc ggcgaagccg
      121 gacgaaaaag acgaccagtt caaggaggca atggcgcgca ccattcccat gggccggccc
      181 cagacccctc aagacatggg ccacgcagcc gtctacctag ccacggccgc caacgtcacc
      241 ggcgtcagcc tgagcgttgc cggcggctac gagatgaatt gaaaaagccg acggccgtgc
      301 agaacctttt ccgcgaaccg ctgctttact tcctgctggc aggcgcggcc atcttcgccc
      361 tcgccgagct cacagacggc cccggcgacg actacctgat cgaggtttcc gacgcggaga
      421 gggcacggat ctcggatcag tggcaggccc agatgggccg ggcgccgacc gacgaggagc
      481 ttgcgggcct gatagagcag tggttgcgcg aagagatcta ctatcgggaa gcccttcgaa
      541 tggggctgga tcgcaacgac atcatcatcc ggcgccgtct tgcccagaag ctcacgttcc
      601 tgaccgagga cgtcattgcc ggttccaccc cctccaccga agagcttcga agctacttcg
      661 aagccaacgc ggagcggtac cgcatacctg agcgaatcac tttccggcac cgctacttca
      721 gcgccgagcg ccggcctaat gccagagagg atgccgccgc cgccctgacg gacgaggccg
      781 tacgcgggga tcccttcatg ctccagaaag cctacgcgct gcgctcgagc cgggagatcg
      841 ccgatctttt cggcgtcgag ttcgccgaag ggctggccca ggtggcgccc gggaactggc
      901 agggaccgat ccgctcggcc tacggatggc acgtggtgct cgtggagcag cgggctcccg
      961 ggcgactgcc gtcgctcgag gaagtgcaga agcgggcggc aacggacctg tcacaggaac
     1021 gccgaagccg agccaacgac gagtactacc actcgctgcg tcagcggtac gagatcatcg
     1081 gcggatgaag gcgctggcgg cgctgctcgt cgttctcgct gcgagccagg ctaaggccca
     1141 cgaggtccgc ccggcctacc tgcagctgac ggaaaccgca ccgggccagt tcgaagtgct
     1201 ctggaagcag ccgttgctcg gcgaccggcg cctgcccctg gaaccggtgc tgcccgaggc
     1261 gtgcgcgcgc gagggcccgc cgatcccgga ggtcgccggc gctgcgctca tcgaacgctg
     1321 gcgcaccgac tgcgaccttc gaagcggagc cgtcaccatc tccggcctct ccaagacgct
     1381 gaccgacgtt ctcgtggaga ttcgctacct gtcggcagag ccagtttccc aattgctcaa
     1441 gcccagcagg ccatccttgg atctggcgtc accgaccccg cagatctgga gctacctcac
     1501 gttcggcgtg gagcacctgc tgctcgggct cgaccacatt ctgttcgtgg tgggcctcgt
     1561 gctattcatc ggcaacacct ggactctggt gaaaaccatc actggcttca ccatcgccca
     1621 cagcatcacg ctggcctgct cggtgctggg gctgatctcg ttgcctcagg cccccgtgga
     1681 agcggtgatc gcgctgtcga tcctctttct tgcccgggaa ctggtggaac ctcccgagcg
     1741 gcgctcgcgc atcaccctgg gccgcccgtg gctgatggcc ttcgctttcg gtctgcttca
     1801 cggcttcggg tttgccggcg cgctcaccga gatcgggctg cccgcagatc agctggccgt
     1861 ctctctgctg ctgttcaacc tcggcatcga gatcggccaa ctcctcgtta tcggcctgct
     1921 ccttagcgct gcggcgattt tcaggaaggc ctggaggcgc aaccgcgagg tctggcccag
     1981 gcggttcgcc tgggtcatgg ggtccgtcgc ggggttctgg accatcgacc gggtgctgct
     2041 cgtcttctga gatcgatctg gctgccgccg cgaacccgat gctcacacgt agacgaagcg
     2101 tcgcaccagc acgtagttgc tggtggtcgc tgccgccgaa ccgatgatca gggcgaccac
     2161 gggcgcctgg gacaaggtgg tgtggagcac cagcgcggtg taggtgccga gggtgatggc
     2221 agcccccagc gactggacca gcacgtagac ggggaacctc gagtcccgga tcgaggcgcc
     2281 gaaggtgtac ctggcgttga gaagaaaggt caccgtcacc gcagcgagga aagaaacaaa
     2341 gcggcttccc acgagccccg tctcggtctc accgagagac attccgtaga ccagcgccga
     2401 gagcacggcc acgtcggtga cgaagcccgc aaagccgacc tgaaggtagc ggaacacctg
     2461 tcggcgggac tgcgtgcgga aatctgtcaa ttcacacctc gtccaggcac cttcacccca
     2521 agcttacgtg gcagccatgt gcccagcaag gcgaccgcct tagcctgccg gcgctttggg
     2581 gttaccatcg ggaaagactc tggggagacg ctcatgacca ccgcacccat cgaatgcccg
     2641 gcacaaaccg tggaggatgg ctgg
//
